LOCUS       DBNGOH010000001         1983 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3299316, whole genome shotgun sequence.
ACCESSION   DBNGOH010000001 DBNGOH010000000
VERSION     DBNGOH010000001.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1983)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1983)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1983
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3299316"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..887)
                     /locus_tag="AC159P_00005"
     CDS             complement(<1..887)
                     /locus_tag="AC159P_00005"
                     /inference="COORDINATES: protein motif:HMM:NF025092.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HIR3693860.1"
                     /translation="MKIYRIEPPISLKLLALLTFFAALALPALAQSNKGTIVGVVTDP
                     NGAVVKDAKVTATNAATGEAREATTNEDGNYTIPALDPGNYRLAIEASGFQNATVEEV
                     KVETNARQAVDVALNVSGVGGDTVIVTAEAPLAETETSVRGDLITGREVTDLPIPQRN
                     FTLLAALSPGVTRPNLGLLGGGGNFVAGGGGQNNTESTRFRESGGSVLVVNGARATNN
                     NFTLDGVDNNEAQFGQIAIFPNPDAIAEFKIETSVPSAESGRAGGGIVQTTFKSGGNE
                     IHGTVYEVYQGRFLSAAASR"
     gene            complement(1196..>1983)
                     /locus_tag="AC159P_00010"
     CDS             complement(1196..>1983)
                     /locus_tag="AC159P_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HIR3693861.1"
                     /translation="IPNEHTDAYARTAKALGVWIQTGTFLERDDRFPGAVFNTTCLIG
                     PEGLIYKYRKVNTWIPYEVHSSPHDLEGYDEPLFPVADTAIGRIGCAVCYDWLFPEAI
                     RQLAANGAEILVRVSAYMDPWGATAPMDWWTVVNRCRAIENTAYVVAANQGASLKHYP
                     PYSWPGGSMVVDFDGRVLAEASPGPGERIVVAPVDISALRHERRARRGHHMLAHLRTE
                     AYPVYRTHLYPPETAARPNEDLSYERNNALIDESKRKLGAPDK"
BASE COUNT          291 a          606 c          707 g          379 t
ORIGIN      
        1 cgagaggcgg cggccgagag gaagcgcccc tggtagacct cgtagaccgt gccgtggatc
       61 tcgttgccgc ccgacttgaa ggtcgtctgg acgatgccgc cgccggcgcg ccccgactcg
      121 gccgagggga cgctcgtctc gatcttgaac tcggcgatgg cgtccgggtt cgggaagatg
      181 gcgatctgcc cgaactgcgc ctcgttgttg tccacgccgt cgagggtgaa gttgttgttg
      241 gtggcgcgcg cgccgttgac taccaagacc gatccgcccg actcgcggaa gcgcgtggac
      301 tcggtgttgt tctggccgcc gccgcccgcg acgaagttgc cgccgccgcc gagcaggccg
      361 aggttcgggc gcgtgacgcc gggcgagagc gccgcgagca gcgtgaagtt gcgctgcggg
      421 atcggcaagt ccgtcacctc gcggcccgtg atcaggtcgc cgcgcacgct cgtctcggtc
      481 tcggcgagcg gggcctccgc cgtcacgatg accgtgtcgc cgccgacgcc gctcacgttg
      541 agcgcgacat cgacggcctg ccgcgcgttg gtctcgactt tgacctcctc gaccgtggcg
      601 ttctggaacc cggaagcctc gatggcgagg cggtagttgc cggggtcgag cgcggggatc
      661 gtgtagttgc cgtcctcgtt cgtcgtagcc tcgcgcgctt cgcccgtggc ggcgttggtc
      721 gccgtcacct tcgcgtcctt gacgaccgcg ccgttcgggt ccgtgacgac cccgacgatg
      781 gttcccttgt tgctctgcgc aagcgcaggg agggcgagcg ccgcgaagaa ggtaagaagc
      841 gcgagcagct tcagagagat tgggggttcg atcctataga ttttcatgtg tgggcctcgc
      901 atgcgtgact gcttgagtgg ccggcgactc gcaatcgtcg cacgcccttt aactctacgg
      961 gggccggtgc cctgccgaga ggcgtgcgga gaaagaggtc ttgagccgaa gacttttgcg
     1021 aaaaactttt ccgaataaga actgaagaag tagccgaggt ttgttgcccg tccgaacgaa
     1081 gtagctttta atacaatttt gtctcgcctg tcaacgattt tctcgaaacg gtcgcgcgaa
     1141 agattctcac acgatttcgt cgcgtgcgtt tcgcggggcg gaaacgcggc tttcgtcact
     1201 tgtccggcgc gccgagtttc ctcttcgact cgtcgatgag cgcgttgtta cgctcgtacg
     1261 agaggtcttc gttcggacgg gcggcggtct cgggcgggta gaggtgtgtg cggtagacgg
     1321 ggtaggcttc ggtgcgcagg tgcgcgagca tgtggtggcc gcggcgcgcg cggcgctcgt
     1381 gcctgagggc ggagatgtcc acgggcgcga cgactatgcg ctcgcccggc cccggcgagg
     1441 cttcggcgag cacgcgcccg tcgaagtcca cgaccatgct cccgcccggc caagagtaag
     1501 gcgggtagtg cttgaggctc gcgccctggt tggcggcgac cacgtaggcc gtgttctcga
     1561 tcgcgcggca gcggttgacg accgtccacc agtccatcgg cgcggtcgcg ccccaagggt
     1621 ccatgtaggc ggagacgcgc acgagtatct cggcgccgtt ggcggcgagc tgccggatgg
     1681 cttccgggaa gagccagtcg taacagaccg cgcagccgat gcgcccgatg gcggtgtcgg
     1741 cgacggggaa cagaggctcg tcgtagcctt cgaggtcgtg cgggctggaa tgcacctcgt
     1801 aaggtatcca ggtattgacc tttcggtact tgtagatcag gccctccggt cctatgaggc
     1861 acgtcgtgtt gaacacggcg cccgggaagc ggtcgtcgcg ctcgaggaac gtcccggtct
     1921 ggatccacac gcccagggcc ttcgccgtcc gtgcgtacgc gtcggtgtgc tcgttgggga
     1981 tcg
//
LOCUS       DBNGOH010000002         5579 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3299392, whole genome shotgun sequence.
ACCESSION   DBNGOH010000002 DBNGOH010000000
VERSION     DBNGOH010000002.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5579)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5579)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5579
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3299392"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(300..1616)
                     /gene="serS"
                     /locus_tag="AC159P_00015"
     CDS             complement(300..1616)
                     /gene="serS"
                     /locus_tag="AC159P_00015"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019005953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HIR3693862.1"
                     /translation="MLDLNYVRGNLEEVRAALEARGFPVAALEDFAALDAERRRVIAE
                     SDALNAQRNTSSREIGGLMKEGRKEEAESRRKEVGELKERIAQLDRARDDAEARMQEL
                     LSTVPNIPHKSVPVGRDESANEEVRQWGEAPKFDFEPQDHVDLGTRLGILDLERAAKI
                     ASARFAILKGAGARLERALINFMLDVHTSEHGYEETLPPFIVNRDSLFGTSQLPKFEG
                     DLFKLEDERGLYLIPTAEVPVTNIHRDEILDAADLPMKFVSYTPCFRSEAGSYGRDTR
                     GIIRQHQFDKVELVKLALPENSYDELEALTRDAETILQRLGIHYRVVALSTGDMGFGA
                     AKTYDIEVWLPSQNTFREISSCTNFESFQARRAQIRFRRAGGAKPEYVHTLNGSGLAV
                     GRTWLAILENYQQPDGSVVIPEALRPYMGGMEKIESSGQQAAGSRQ"
     gene            complement(1653..2408)
                     /locus_tag="AC159P_00020"
     CDS             complement(1653..2408)
                     /locus_tag="AC159P_00020"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020494050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HIR3693863.1"
                     /translation="MKLIILAAGYATRLYPLTLNQPKPLLEVAGKPMIEHVLDNLAGI
                     EEIDRVHVVTNDKFAGHFQRWADEYGADKGGAGFTITNDRSTDDSNKLGAIGDLNLVL
                     TRGEIDDDIIVVAGDNLFSESLEDFARFAREKGAPVLAVYDVGDLEEIKKYNSIEVDE
                     EGRITFFEEKPAHPKSTLTGIALYYYPQSTLPLIRQYVEEGNNPDQPGRLVQWMYPRT
                     PFHTWRVPGIWYDIGSKENLEEAGRIFAKRDDG"
     gene            2678..3439
                     /gene="fabG"
                     /locus_tag="AC159P_00025"
     CDS             2678..3439
                     /gene="fabG"
                     /locus_tag="AC159P_00025"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017813633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HIR3693864.1"
                     /translation="MSEETKPFAGSVGIVTGATRGIGRAIALELARRGASVAFNYAQS
                     AEAAETLKSEIEALGTQALAAQCDVANTEAAAEMVKQVKDTFGRIDFLVNNAGITRDN
                     LILRMKEDDWDAVIDTNLKGAWNFSRAALRHMLRQEGGGAVVNISSISGVVGMTGQSN
                     YSASKAGMIGLTKALAKEVASRNVSVNALALGLVATDMSHALDESYRRKLLENIPMRR
                     FAEPGEVAKIACFLLSQDARYITGQVIQADGGLAM"
     gene            complement(3982..4980)
                     /locus_tag="AC159P_00030"
     CDS             complement(3982..4980)
                     /locus_tag="AC159P_00030"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015251.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HIR3693865.1"
                     /translation="MGRKSKGQIFFEYAVARAFLTGLGALPRPRAVAAGRGLGRAAYA
                     LAAGLRRTGLRNLELAFPEKSVREREEILRGSFVSLGRQLGEVSQFPRANAERLRAVV
                     EYDDEDVKLLDVARTRGRGVIFLTSHLGAWEILCFAHSVFHDPLSFLVRPIDNPRIDK
                     MIETLRTRFGNRPIDKKAAARTAMKLLRQGGTLGVLADLNTQEREGVFVPFFGHLACT
                     TAGVATLALRTDATVIPVCAPWNDERKKFVFRGGPVLELVRTGDHERDVQLNTARFTA
                     AIEAHVRAYPDQWLWIHKRWKTRPAGEPDLYARPTKKSDGDVTFSPPTPTEKIFKT"
     gene            5149..>5579
                     /locus_tag="AC159P_00035"
     CDS             5149..>5579
                     /locus_tag="AC159P_00035"
                     /inference="COORDINATES: protein motif:HMM:NF021503.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein"
                     /protein_id="HIR3693866.1"
                     /translation="MPSGKTHDAITLVLAAPTFAAAWGLTGSLRLATAATCAMLFGGF
                     MFGPDLDILSRQYTRWGVFRFLWFPYKVVFRHRSRWSHGIIFGTLIRVVYFASALALL
                     GWAGVYLRAVYVTGGAAPGWADLAHAWQLIEAGAARYGLGSA"
BASE COUNT          938 a         1751 c         1853 g         1037 t
ORIGIN      
        1 ggcgacgaga cgagcgtcaa ggcgcgcgag cagcagacgc tcatcaactc cgcccccaga
       61 gagatgcgcc gcgccctgcg ccacgccgcc gtcttcgagc gcttccgcca gggcgaagcg
      121 ctcgccatca tgccgttcga tctgaaaatc gaatagcggg cagcgggcag caggcagcag
      181 gcagtaaaga caaaaagcga agaggcttga gatgttggct ctcaagcctc ttcgttcatc
      241 aagcaacaag aggacgatgc ttgagaggta aagttccaag catcgtcgtc ctgccgcctt
      301 tactgcctgc tgcccgctgc ctgctgcccg ctgctttcta tcttctccat cccgcccatg
      361 tacggacgga gcgcttcggg gatgacgacc gagccgtccg gctgctggta gttttcgagg
      421 atggcgagcc acgtgcggcc cacggcgagg ccggagccgt tgagcgtgtg gacgtattcg
      481 ggcttggcgc cgcccgcgcg gcggaagcgt atctgcgcgc ggcgtgcctg gaaggactcg
      541 aagttggtgc acgaagagat ttcgcggaag gtgttctgcg agggcagcca gacttcgatg
      601 tcgtaggtct tggcggcgcc gaagcccatg tcgccggtcg agagcgcgac gacgcggtag
      661 tggatgccga gccgctgcaa gatggtctcg gcgtcgcgcg tcaacgcttc cagctcgtcg
      721 taggagtttt ccggcagcgc gagcttgacc agctccacct tgtcgaactg gtgctggcgg
      781 atgatgccgc gcgtgtcgcg cccgtaggag ccggcctcgg agcggaagca gggcgtgtac
      841 gagacgaact tcatcggcag gtcggcggcg tccagtatct cgtcgcggtg gatgttcgtg
      901 acgggcactt ccgccgtcgg gatcagatac agcccgcgct cgtcttcgag cttgaagaga
      961 tcgccctcga acttcggcaa ctgcgacgtg ccgaacagcg agtcgcggtt gacgatgaag
     1021 ggcggcagcg tctcctcgta gccgtgctcc gaggtgtgaa cgtccagcat gaagttgatg
     1081 agcgcgcgtt ccagccgcgc acccgcgccc ttcaggatgg cgaagcgcgc gctcgcgatc
     1141 ttcgccgcgc gttcgaggtc gaggatgccg agccgcgtgc cgaggtcaac gtgatcctgc
     1201 ggctcgaagt cgaacttggg agcctcgccc cactggcgca cctcttcgtt cgccgactcg
     1261 tcgcgcccga ccggcaccga cttgtgcggg atgttcggga cggtggacag cagttcctgc
     1321 atccgcgctt cggcgtcgtc gcgcgcgcgg tcgagctgcg cgatgcgctc cttcagctcg
     1381 ccgacctctt tgcgccgcga ctcggcctct tctttgcggc cctccttcat cagcccgccg
     1441 atctcgcggc tcgaagtgtt gcgctgcgcg ttcaaggcgt ccgactcggc gatgacgcgg
     1501 cggcgctccg cgtcgagcgc cgcgaagtct tcgagcgcgg ccaccggaaa gccgcgcgcc
     1561 tcaagcgccg cacgcacctc ttcgaggttc cccctcacgt agttcaaatc gagcattcgt
     1621 cttcgtccct ctcgcctgtc gtcgagaaca ggtcagccgt cgtcgcgttt ggcgaagatg
     1681 cggccggcct cttcgaggtt ctccttcgag ccgatgtcgt accagatgcc gggcacccgc
     1741 cacgtgtgga aaggcgtgcg cgggtacatc cactggacga ggcggcccgg ctggtcgggg
     1801 ttgttgccct cctcgacgta ttgacggatg agcggcagcg tcgattgcgg ataataatag
     1861 agcgcgatgc cggtgagcgt gctcttcggg tgcgcgggct tctcctcgaa gaaggtgatg
     1921 cggccctctt cgtccacctc gatggagttg tacttcttga tctcttcgag gtcgcccacg
     1981 tcgtagacgg cgagcacggg cgcgcccttc tcgcgcgcga accgcgcgaa gtcctcgagg
     2041 ctctcgctga agaggttgtc gcccgcgacg acgatgatgt cgtcgtcgat ctcgcctcgc
     2101 gtcagcacca gattcaagtc gccgatggcg ccgagcttgt tcgagtcgtc ggtcgatcgg
     2161 tcgttggtga tcgtaaagcc ggcgccgccc ttgtcggccc cgtactcgtc cgcccaacgc
     2221 tggaagtgtc cggcgaactt gtcgttggtg acgacgtgga cgcggtcgat ctcttcgatg
     2281 cccgcgaggt tgtcgagcac gtgctcgatc atcggcttgc ccgccacctc aagcagcggc
     2341 ttcggctggt tcaacgtcag cgggtagagg cgcgtcgcgt agcccgccgc caaaattatc
     2401 agtttcatct ctctcccttc gcccgcgcgc ctccggcccg atcttcgaga cggagggctt
     2461 gcgcgtccct cttgcgtgcg cgcgcggcct tgcgctcttt gccttcggat gaatctctgc
     2521 ccaaatcttt acaataaaaa cagggccggg gcaaacgaag ccgcgcgcgg cccgccgcaa
     2581 ggtcgagggc ggccccgctc gcgcgcgggg cgaagctctg ctatattgcg ccgggccgcg
     2641 ggcgttcgag ggcggcgtct tcggaggaga aaaacttttg agtgaagaga cgaaaccttt
     2701 cgcgggcagc gtcggcatcg tgacgggcgc gacgcgcggc atcggtcgcg ccatcgcttt
     2761 ggaactggcg cggcgcggcg cctcggtcgc cttcaactac gcgcagagcg cggaggccgc
     2821 cgagaccctg aagtcggaga tcgaagccct cggcacgcag gctttggccg cgcagtgcga
     2881 cgtggcgaac accgaagcgg cggcggagat ggtcaagcag gtcaaggaca ccttcgggcg
     2941 catcgacttc ctcgtcaaca acgccggcat cacgcgcgac aacctgatcc tccgcatgaa
     3001 ggaggacgac tgggacgccg tcatcgacac gaacctgaag ggcgcctgga acttctcgcg
     3061 cgcggcgctg cgccacatgc tccggcagga gggcggcggc gcggtcgtca acatctcttc
     3121 gatcagcggc gtcgtcggga tgacggggca gtcgaactac tccgcgtcga aggccggcat
     3181 gatcgggctg acgaaggcgc tcgccaagga ggtggcgagc cgcaacgtct ccgtcaacgc
     3241 gctcgccctc ggcctcgtcg ccaccgacat gtcgcacgcg ctcgacgaat cctaccgccg
     3301 gaagctcctc gaaaacatcc ccatgcgccg cttcgccgaa cccggcgaag tcgccaagat
     3361 cgcctgcttc cttctctcgc aggacgcgcg ctacatcacc gggcaggtga tacaggccga
     3421 cggcgggttg gcgatgtaac gattgcggat tttagattgc ggattgcgga ttgaccgggc
     3481 gccgatggtt gaatgctgac atcaggctta acgcctgaga agcacgtcaa tccgcaatct
     3541 aaaatccgca atagaaagaa aggtgccccg ccggttctag ggttaaaccg acggggcgca
     3601 tcttcgggca ggctcggaga ttcagggtgg cgttgtctat tcgctggaga tagtcaccta
     3661 cgggctatcc ggctttgggg gcggtcgttg ggatgggcga gataaaacct gtcggtaact
     3721 tactcaatct caacgagagc ttatggaacc gaagaaacgc tcttgcccgt aagattgaag
     3781 aaacccaacg actgcctttt caatcagctt tgaagcacca cctcgaactt atctgcttcg
     3841 tgacttgtgt aaaattacac tgaacgcctg tgacttgtca agcataaaat acaaaatttc
     3901 tacacaattt cgtaacagct cgggccgccc tacctaaatc gctgagcctg caaggttaaa
     3961 gtctcacaaa taagttccgg ttcaggtctt gaaaatcttc tcggtcggcg tcggagggga
     4021 aaatgtcacg tcgccgtcac tttttttcgt cgggcgtgcg tagagatcag gctcgccggc
     4081 ggggcgcgtc ttccagcgtt tgtgaatcca gagccattgg tcggggtagg cgcggacgtg
     4141 tgcttcgatg gcggcggtga agcgcgcggt gttgagctga acgtcgcgct cgtggtcgcc
     4201 cgtgcggacg agttcgagga cggggccgcc gcggaagacg aacttcttac gctcgtcgtt
     4261 ccagggggca cagacgggga tgacggtggc gtcggtgcgc agcgcgagcg tggcgacgcc
     4321 ggcggtggtg caggcgaggt ggccgaagaa ggggacgaag acgccttcgc gctcctgcgt
     4381 gttgaggtct gcgaggacgc cgagcgtgcc gccctgccgg aggagcttca tggccgtgcg
     4441 cgcggcggcc ttcttgtcga tgggccggtt gccgaagcgc gtccgcaggg tttcgatcat
     4501 tttgtcgatg cgcgggttgt cgatagggcg gacgaggaag cttagggggt cgtggaagac
     4561 cgagtgcgcg aagcagagaa tctcccacgc gccgaggtgc gaggtgagga agatgacgcc
     4621 gcgcccacgc gtccgagcca cgtcgaggag cttcacgtct tcgtcgtcgt actcgacgac
     4681 tgcgcgcagc cgctcggcgt tcgcgcgcgg gaactggctc acctcgccga gctgccgccc
     4741 gagactcacg aacgagccgc gcaaaatctc ctcgcgctcg cgcacgctct tttcggggaa
     4801 ggcgagctcg aggttacgca ggcccgtgcg cctgaggccg gcggcgagcg cgtaggccgc
     4861 gcggcccagg ccgcgccccg ccgccacggc gcgcggacgc ggcagcgcgc cgaggcccgt
     4921 caggaacgcc cgcgccaccg cgtactcgaa gaagatttgc cctttgcttt ttctgcccat
     4981 acgacaaggc cgacagtgta accgcgcgct acgtactcag gcaaaccgct ggcacggcgc
     5041 gcgcggcaca tgctataacc ccggaagaca ggagtcagag gtcagaagtc agaataaaaa
     5101 agaacggatg ggtttcttct gacttctgac ttctgactcc tgctttctat gccttccggg
     5161 aagacacacg acgcgatcac gctggtgctg gcggcgccga cgttcgccgc cgcgtggggg
     5221 ctgacgggga gcctccggtt ggcgacggcg gcgacgtgcg cgatgctgtt cggcggtttc
     5281 atgttcggcc cggacttgga catcctgtcg cggcagtaca cgcgttgggg cgtgttccgg
     5341 ttcctgtggt tcccttacaa ggtcgtcttc cgccaccgct cgcggtggtc gcacggcatc
     5401 atcttcggga cgctcatccg cgtcgtctac ttcgcgtccg cgctcgcgct gctcggatgg
     5461 gccggcgtct acctgcgcgc cgtgtacgtg accggcgggg cggcgcccgg ctgggccgac
     5521 ctcgcgcacg cgtggcaact catcgaggcg ggcgcggccc gctacggcct cggctcggc
//
LOCUS       DBNGOH010000003         2659 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3849296, whole genome shotgun sequence.
ACCESSION   DBNGOH010000003 DBNGOH010000000
VERSION     DBNGOH010000003.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2659)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2659)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2659
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3849296"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..777
                     /locus_tag="AC159P_00040"
     CDS             <1..777
                     /locus_tag="AC159P_00040"
                     /inference="COORDINATES: protein motif:HMM:NF018387.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 family protein"
                     /protein_id="HIR3693867.1"
                     /translation="CHSTAPPRALAESKTSAAAPAPAAPVIEAARWDDSDSDGLPDRA
                     ELHSYNDRENFRRWFTAIAEQQFYAASGEWNAAQRDCAGLVRFAWREALRPHDRAWVR
                     RMGEGYDAVAPDVRAYTLERGPLGEKIFRTDFGGFAESDLREGKFSEFADARTLKDFN
                     TEFVSRDRREARAGDLLFFHQPWVQKYPYHVMLYLGAAREEAEGAGDWVVYHTGASAE
                     DPGEVRKLRLSTLDQHPDPRWRPLERNRHFLGFYRLKILE"
     gene            883..>2659
                     /locus_tag="AC159P_00045"
     CDS             883..>2659
                     /locus_tag="AC159P_00045"
                     /inference="COORDINATES: protein motif:HMM:NF023400.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HIR3693868.1"
                     /translation="MTRRTVLSSIACYLGLVLLVAGFAVLRVRTQNRFAAQTAPDSLP
                     MYGREITVPDPHAKPFFSIQTSRTYSTSDRARVSINYRGVRSLDFRVYKVKDPVKFFR
                     ELEDPHRIGEDEEAKAAELTKRKPTFLENLRQFKSYVYATIKRYVRSQLQNQSRKGFN
                     QRFRVVAEEEETPSVRTPLNVGELARVPLLNPDQMIDSWREELPPLEDAYDRRTISLG
                     RREPGVYLVEVVNGDLRAFGLAIVTDIAMVEKSANDGQTLFYVVERKSGAPRAGVNVQ
                     IIRGKDDITKGTTDQQGILRMQLVRKPQPTEEEEEDEEAPEEEAAAVDTPYLVTATDG
                     NDFAISDLNSFYFQGDGGEGEDGGGAELTSYLYTDRPVYRPEQKVYFKGILRQLTDQG
                     YKLLPDSTVSVSVEDQSGGRIYEQELKLSTRGTFSGELDLPEESPLGYYTINAQAGDA
                     SASGGFEVQEYKKPEFKVKVTTPRAHVNVGEKTSFTVSARYFFGAPVARASVKYYVYR
                     SRHYGWWDAGGEAEEDEFGADPSRDEDESSGYSGYDDNMVLEGEGKLDAQGNLNVEFT
                     TPQPKEKETWDYEYRLDAQVTDASRR"
BASE COUNT          497 a          930 c          857 g          375 t
ORIGIN      
        1 tgccacagca ccgcgccgcc ccgcgcctta gccgaaagca agaccagcgc cgccgccccc
       61 gccccggcag cgcccgtgat tgaagccgcg cgctgggacg actcggactc cgacggcctg
      121 cccgaccgcg ccgagcttca ctcctacaac gaccgcgaaa acttccgccg ctggttcacg
      181 gccatcgccg agcaacagtt ctacgccgcg agcggagagt ggaacgcggc gcagcgcgac
      241 tgcgccgggc tggtgcgctt cgcgtggcgc gaggcccttc gcccgcacga ccgcgcgtgg
      301 gtgcggcgga tgggcgaggg ctacgacgcg gtggcgccgg acgtgcgcgc ctacacactg
      361 gagcgcgggc cgctcggcga aaaaatcttc cgcacggact tcggcggctt cgcggagtcg
      421 gacttgaggg agggcaagtt ctccgagttc gccgacgcgc gcacgctcaa ggacttcaac
      481 acggagttcg tcagccgcga ccgccgcgag gcgcgcgcgg gcgacctgct cttcttccac
      541 cagccgtggg tgcagaagta cccgtaccac gtgatgcttt acctgggcgc cgcgcgcgaa
      601 gaggcggagg gcgcgggcga ctgggtcgtc taccacacgg gcgcctcggc ggaagacccc
      661 ggcgaggtca ggaagctgcg cctctcgacg ctcgaccaac accccgaccc gcgctggcgc
      721 cccctcgaac gcaaccgcca cttcctcggc ttctaccgct tgaagatact ggaatgaaaa
      781 acagaagtca gaaatcggaa gtcggaagtc agaagaaagg cggcgaccgc tttttcattc
      841 tgacttccga cttctgactt ctgacttctt ctttgagggc ttatgactcg tcgaaccgtc
      901 ctttcgtcaa tcgcgtgtta cctcgggctg gtgctgctgg tggcgggctt cgccgtgttg
      961 cgcgtgcgga cgcagaaccg cttcgcggcg cagacggcgc ccgactcgct gccgatgtac
     1021 gggcgcgaga tcaccgtgcc cgacccgcac gccaagccct tcttctcgat ccagacgagc
     1081 cgcacctact cgacgagcga ccgcgcgcgc gtctccatca actaccgcgg cgtccggagc
     1141 ctcgacttcc gcgtctacaa ggtcaaagac ccggtgaagt tcttccgcga gctggaagac
     1201 cctcaccgca tcggcgagga cgaggaggcg aaggccgccg agctgaccaa gcgcaagccg
     1261 accttccttg agaacctgcg ccagttcaag agctacgtct acgccacgat caagcgctac
     1321 gtccgctcgc agctccagaa ccagtcgcgc aagggcttca accagcgctt ccgcgtcgtc
     1381 gccgaggagg aggagacgcc gagcgtccgc acgccgctca acgtcggcga gctggcgcgc
     1441 gtcccgctcc tcaaccccga ccagatgatc gacagttggc gcgaggagct gccgccgctc
     1501 gaagacgcct acgatcggcg caccatctcg ctcgggcggc gcgagccggg cgtctacctc
     1561 gtcgaggtgg tcaacggcga cctgcgcgcc ttcggcctcg ccatcgtcac cgacatcgcg
     1621 atggtcgaga agtccgcgaa cgacgggcag acgctcttct acgtcgtcga gcgcaagtcg
     1681 ggcgcgccgc gcgcgggcgt caatgtccag atcatccgcg gcaaggacga catcacgaaa
     1741 ggcacgaccg accagcaggg catcctccgc atgcagctcg tgcggaagcc gcagccgacc
     1801 gaagaggagg aagaagacga agaggcgccg gaagaggaag cggcggcggt cgataccccc
     1861 tacctcgtca cggcgaccga cggcaacgac ttcgccatct ccgacctcaa ctccttctac
     1921 tttcagggcg acgggggcga gggcgaagac ggcggcggcg ccgagctgac gagctacctc
     1981 tacaccgacc ggcccgtcta ccggcccgag cagaaggtct acttcaaagg catcctccgc
     2041 cagttgaccg atcagggcta caagctgctg cccgactcga ccgtcagcgt ctcggtcgaa
     2101 gaccagagcg gcgggcgcat ctacgagcag gagctgaagc tctcgacgcg cggcaccttc
     2161 agcggcgaac tcgacctgcc ggaagagtcg ccgctcggct actacaccat caacgcgcag
     2221 gcgggcgacg cctcggcctc cggcggcttc gaggtgcagg agtacaagaa gcccgagttc
     2281 aaggtgaagg tgacgacgcc gcgcgcgcac gtcaacgtcg gcgagaagac ctcgttcacg
     2341 gtctcggccc gctacttctt cggcgcgccg gtcgcgcgcg cgagcgtcaa gtactacgtc
     2401 taccgctcgc gccattacgg ctggtgggac gcgggcggcg aggccgagga ggacgagttc
     2461 ggcgccgacc cttcgcgcga cgaggacgag tcctccggct actccggcta cgacgacaac
     2521 atggtgctcg aaggcgaggg caagctcgac gcgcagggca acctcaacgt cgagttcacg
     2581 acgccgcagc ccaaagagaa agagacgtgg gactacgagt accgcctcga cgcgcaggtc
     2641 acggacgcct cgcgccgct
//
LOCUS       DBNGOH010000004         1932 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_550028, whole genome shotgun sequence.
ACCESSION   DBNGOH010000004 DBNGOH010000000
VERSION     DBNGOH010000004.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1932)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1932)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1932
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_550028"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            124..>1932
                     /locus_tag="AC159P_00050"
     CDS             124..>1932
                     /locus_tag="AC159P_00050"
                     /inference="COORDINATES: protein motif:HMM:NF018899.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HIR3693869.1"
                     /translation="MRFKGFIFVFVALLLAPSGARALSRNNVPDLSRYEGRTVASVEV
                     SVEEASVEESALAEFRAVLKIAEGNRFSAVLVRDSLQALFDTNRVAGARVEASDAAGP
                     PGEGGRPRVALRFVVKPQVLLSDVEFEVGVTEGTGISEDELRGRINLLEPGRRLTRQA
                     VEQNADLIQVYLRDRGFYRATVESEQRLDPTRTRATVVFRVEPGPPTTVEAFNIDIEG
                     FDPAALTRLRPDLRLQTGARFTLEALGEDVARVRQAVLAEGRLAPRIEEPDVRLDSQT
                     NRITIGITGAVGPEVKVEIEGYELSEKRQRELLPILREGSVEYSAIVEGERRMENRLQ
                     EEGYFFADVTPVCSVQPPFAPPAPTAAPPVNGEEELDTCQLLSPEDLTGRSVAIKYMV
                     ERGRRFELTDIRIEGTEKLSFEDVEDDLRSRTANVLGLIPFVGLGRGYTSADALERDR
                     RLIEARVKDLGHRSAAVTVRQGVSVAGDNLIITFAVEEGPLTRVASVETRGNQIYTER
                     QLGDGRCPPEQTPEDACLVVGGPYSRTQGRTDAERVRAYYSRNGYLDSDVDLDVVELP
                     PDRVTGDERVRLIYNIAESDKVFVNRIIVNGNVLTKR"
BASE COUNT          311 a          695 c          634 g          292 t
ORIGIN      
        1 gggcagcagg cagttaatac acttcagatt gcagatatga gatttgaact ttcaaagtaa
       61 caatccggaa tctttatttt tactgcctgc tgcctgctgc ccgctgcccg ctgattatca
      121 tccgtgagat tcaaggggtt catcttcgtc ttcgtcgcgt tgctgctcgc gccttcgggc
      181 gcgcgggctt tgtcgcgcaa caacgtcccc gacctcagcc gctacgaggg gcgcacggtc
      241 gcgtcggtcg aggtgtcggt cgaagaggct tcggtcgaag agtcggcgct cgccgagttc
      301 cgcgccgtgc tcaagatcgc cgagggcaac cgcttctcgg cggtgctcgt gcgcgactcg
      361 ctgcaagcgc tcttcgacac gaaccgcgtg gcgggcgcgc gcgtcgaggc gtcggacgcc
      421 gccgggccgc cgggcgaggg cggtcgcccg cgcgtcgcgc tgcgcttcgt cgtcaagccg
      481 caggtcctgc tctcggacgt ggagttcgag gtcggcgtca ccgaagggac gggcatctcg
      541 gaggacgagc tgcgcgggcg catcaacctg ctggagccgg gccggcgtct gacgcggcag
      601 gccgtcgagc agaacgcaga cctcatccag gtctacctgc gcgaccgcgg cttctaccgc
      661 gccacggtcg agagcgagca gcggctcgac ccgacgcgta cgcgcgccac cgtcgtcttc
      721 cgcgtcgagc cggggccgcc caccaccgtc gaagccttca acatcgacat cgagggcttc
      781 gacccggcgg cgctcacgcg gctgcgcccc gacctccgtc tccaaacggg cgcgcgcttc
      841 accctcgaag ctttgggcga ggacgtggcg cgcgtccgcc aggccgtcct cgccgaaggg
      901 cgcctcgcgc cgcgcatcga ggagccggat gtgcggctcg actcgcagac caaccgcatc
      961 accatcggca tcacgggcgc cgtcgggccg gaggtcaagg tcgagatcga gggctacgag
     1021 ctgagcgaga agcggcagcg cgagctgctg cccatccttc gcgaaggctc ggtcgaatat
     1081 tcggccatcg tcgagggcga gcgccgcatg gagaaccgcc ttcaggagga gggctacttc
     1141 ttcgccgacg tgaccccggt ctgttccgtc cagccgccgt tcgcgccgcc cgcccccacc
     1201 gccgccccgc ccgtcaacgg cgaagaagaa ctggacacct gccagctcct cagccccgaa
     1261 gacctgacgg gccgcagcgt cgcgatcaag tacatggtcg agcgcgggcg ccgcttcgag
     1321 ctgaccgaca tccgcatcga gggcaccgag aagctctctt tcgaagacgt ggaggacgac
     1381 ctcaggagcc gcacggccaa cgtgctcggc ctcatcccct tcgtcggttt gggccggggc
     1441 tacacgagcg ccgacgcgct ggagcgcgac cgccgcctga tcgaggcgcg cgtgaaagac
     1501 ctcggccacc gcagcgccgc agtcaccgtc cggcagggcg tctcggtcgc gggcgacaac
     1561 ctcatcatca ccttcgccgt cgaggagggg ccgctcacgc gcgtcgcctc cgtcgagacg
     1621 cgcggcaacc agatttacac cgagcggcaa ctcggcgacg ggcgctgccc gcccgagcag
     1681 acgcccgagg acgcctgcct cgtcgtcggc ggcccgtact cgcgcacgca agggcggacg
     1741 gacgccgagc gcgtccgcgc ctactactcg cgcaacggct acctcgactc ggacgttgac
     1801 ctcgacgtgg tcgagctgcc gcccgaccgc gtgaccggcg acgagcgcgt ccgcctcatc
     1861 tacaacatcg ccgagtccga caaggtcttc gtcaaccgca tcatcgtcaa cggcaacgtg
     1921 ctgaccaagc gc
//
LOCUS       DBNGOH010000005         2524 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1466605, whole genome shotgun sequence.
ACCESSION   DBNGOH010000005 DBNGOH010000000
VERSION     DBNGOH010000005.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2524)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2524)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2524
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1466605"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..280
                     /locus_tag="AC159P_00055"
     CDS             <1..280
                     /locus_tag="AC159P_00055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3693870.1"
                     /translation="TYASRAAQQRAYEQQRAYEQRQREYYDDRYDDDRSVFEKHRDKL
                     TVAGGTVAGAAVGAATGGKKGAIIGALVGAGGSALYTYKLRDKNGRRY"
     gene            433..1002
                     /locus_tag="AC159P_00060"
     CDS             433..1002
                     /locus_tag="AC159P_00060"
                     /inference="COORDINATES: protein motif:HMM:NF025173.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0044183 - protein folding chaperone
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HIR3693871.1"
                     /translation="MNGFGKIKTLAVGSLAAIALAAPIAIAQTGTTSGQDGQQTPRAE
                     RRGFGKRGGDHRGGRGWGGRGFGFRGIELTEDQKTRMKQLHESFGERTKSLHEQLRAK
                     RTELRQAEQGTSFNEALAAQKLTESAAIEAKLMGERFRLRQETLAVLTAEQRTQLEQQ
                     RAQREQRREERKNRRGTRRGQGGQVEQMQ"
     gene            1102..1722
                     /locus_tag="AC159P_00065"
     CDS             1102..1722
                     /locus_tag="AC159P_00065"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR3693872.1"
                     /translation="MERASDLELAGRSARGDDDAFAEIVRRYGPRVFRVVGRYFRQRD
                     RAEEAAQEVFLKAFTQLDAYEGRGSMEGWLTRIATTTCLNLLRSAKRRPESTISDLTE
                     DEGAWLETHTASAAAEQHRAVENSVVAADLAERVLDTMSHDDRLVLTLVDGEELAVKE
                     VAEMTGWSESKVKVQAFRARRRMRAAVEKLLAFGGRNRGDSKHAAE"
     gene            1744..2247
                     /locus_tag="AC159P_00070"
     CDS             1744..2247
                     /locus_tag="AC159P_00070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3693873.1"
                     /translation="MRDEHVKGILDAAPLAQLGEGELAAVRAHTARCAECLRAYEAAR
                     ASSLLLTERVAAAAEVEMPPFFQTRVLAALRERRATEEAPALSRLWRAAGALVSSMAV
                     TVAALVALTFTVPGTFTMQETTEMVAASDLSPDEAALLALDDVAAEDEIDYGQVLTTV
                     YEMEDER"
     gene            2250..>2524
                     /locus_tag="AC159P_00075"
     CDS             2250..>2524
                     /locus_tag="AC159P_00075"
                     /inference="COORDINATES: protein motif:HMM:NF025173.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic heavy metal sensor"
                     /protein_id="HIR3693874.1"
                     /translation="MDRIAKNRWQVRLAALVIFLLGVAAGALALNVYNAWSRASGRDG
                     GRGGFEQMLDRLELTGEQRTQVQQLLADTRKQFREARRDSEPRMEDIR"
BASE COUNT          442 a          812 c          936 g          334 t
ORIGIN      
        1 cacttacgct tcgcgtgcgg cgcagcagag ggcttacgaa cagcagaggg cttacgagca
       61 gcggcagcgt gagtactacg acgaccgcta cgacgacgac cgttcggtct tcgagaagca
      121 tcgagacaag ctgacggtgg cgggcgggac ggtcgcgggc gcggcggtgg gtgcggcgac
      181 cggcggcaag aagggcgcca tcatcggcgc gctcgtcggc gcgggcggct cggcgctcta
      241 cacctacaag ctgcgcgaca agaacgggcg gcgctactga aatcggcgcg cgcctgtaac
      301 gcttcggcgg cgggcggtgt ctgacagggt gcggggccag ctctcctgac agagcttgcc
      361 ccgctcataa acgcaaaacg ggactcctcc cctcgaacaa gaacaaggta aactaaggag
      421 cacgaatcag tgatgaatgg atttgggaaa atcaagacgc tggcggtcgg ttcgctggca
      481 gccatcgcgc tggccgcgcc gattgccatc gcgcagaccg gcacgacgag cgggcaggac
      541 ggccagcaga cgccccgtgc cgagcggcgg ggcttcggca agcgcggcgg cgaccaccgc
      601 ggcgggcgcg gctggggcgg gcgcggcttc ggcttcaggg gcatcgagct gaccgaagac
      661 cagaagacgc gcatgaagca gcttcacgag agcttcggcg agcgcacgaa gtcgctgcac
      721 gaacagctcc gcgccaagcg caccgagctg cgtcaggccg agcagggcac ctcgttcaac
      781 gaggcgctgg ccgctcagaa gctgacggag tccgccgcca tcgaagcgaa gctgatgggc
      841 gagcgcttcc gtctgcggca ggagacgctg gccgtgctca ccgccgagca gcgcacgcag
      901 ctcgaacagc agcgcgcgca gcgcgagcag cgccgcgaag agcgcaagaa tcgccgcggc
      961 acgcgccgcg gtcagggcgg tcaggtcgag cagatgcagt aacgctcgat cacacgcgct
     1021 cggcggcggg cttcggcagc ttcaggcgaa ccggagtccg ccgcccgcga aacttttcgg
     1081 ggtgcgcctc tgcgggacga cttggagcgg gcgtcggact tggagttggc gggccgctcg
     1141 gcgcgaggcg acgacgacgc cttcgcggag atcgtgcgcc gctacggccc gcgcgtcttc
     1201 cgcgtcgtcg ggcggtactt ccgccagcgc gaccgcgccg aagaggccgc gcaggaggtc
     1261 tttctgaaag ccttcacgca gctcgacgcc tacgaggggc gcggctcgat ggaaggctgg
     1321 ctgacgcgca tcgccaccac gacgtgcctc aacctgctcc gcagcgcgaa gcgtcggccc
     1381 gagtcgacca tctcggactt gaccgaggac gagggcgcgt ggctcgaaac gcacacggcg
     1441 agcgcggcgg ctgagcagca ccgcgcggtc gagaatagcg tcgtcgcggc agacctcgcc
     1501 gagcgcgtgc tggacacgat gtcgcacgac gaccgtctgg tgttgacgct ggtggacggc
     1561 gaggagctgg ccgtcaaaga ggtggccgag atgacgggct ggtccgagtc gaaggtgaag
     1621 gtgcaggcgt tccgcgcgcg ccgaaggatg cgcgcggcgg tggagaagct gctcgcgttc
     1681 ggcggcagga atcgcggcga cagcaagcac gccgcagagt aagtttgaaa gcgcaggtta
     1741 gagatgagag acgaacacgt taaaggcatc ctcgacgccg cgccgctcgc gcagctcggc
     1801 gagggcgaac tggccgccgt gcgcgcacac acggcgcgct gcgcggagtg cctgcgcgcc
     1861 tacgaggccg cgcgcgcttc gtcgctgctg ttgacggagc gcgtcgccgc cgccgccgag
     1921 gtcgagatgc cgccgttctt ccagacgcgc gtgctggccg cgctgcgcga gcggcgcgcg
     1981 acggaagagg cgcccgcgct cagccgcctg tggcgcgcgg cgggcgcgct cgtctcctcg
     2041 atggcggtca cggtcgcggc gctcgtcgcg ctgacgttca ccgtccccgg cactttcacg
     2101 atgcaggaga cgacggagat ggtcgctgct tccgatcttt cgccggacga ggccgcgctc
     2161 ctcgcgctgg acgacgtggc ggcggaagac gagatcgact acgggcaggt gctcacgacc
     2221 gtctacgaga tggaagacga gaggtgacga tggacagaat cgctaagaac aggtggcagg
     2281 ttcgcttggc cgcgctcgtc atctttctgc tcggcgtcgc ggcgggcgcg ctcgcgctga
     2341 acgtctacaa cgcgtggtcg cgcgcctctg gccgcgacgg ggggcgcggc gggttcgagc
     2401 agatgctcga ccgccttgag ctgaccggcg agcagcggac gcaggtgcaa cagttactgg
     2461 ccgacacgcg caaacagttc cgcgaagccc gccgggactc cgagcctcgc atggaagaca
     2521 tccg
//
LOCUS       DBNGOH010000006        16063 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_184211, whole genome shotgun sequence.
ACCESSION   DBNGOH010000006 DBNGOH010000000
VERSION     DBNGOH010000006.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 16063)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 16063)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16063
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_184211"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(208..1554)
                     /gene="glmM"
                     /locus_tag="AC159P_00080"
     CDS             complement(208..1554)
                     /gene="glmM"
                     /locus_tag="AC159P_00080"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR03990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HIR3693875.1"
                     /translation="MNSLKIGISGVRGVVGETFTPELAVGFAQAFGTYLDSGRILVCR
                     DARASGPMVRAAVLAGLLAAGCEVFDLGICPTPSLQLGVRRLGAVGGISITAGHNPEP
                     WNALKFVRSDGLYLNATQADELLDIYHQAEFAKATWADIHSRVERADAIPHHLETLSD
                     AFDVEAIRSRRLTVAVDCCNSSCALLSPRWLASLGCEVFAVNDDPAMPFPHAPEPRRQ
                     TMAQLRAVVKAGRADIGFAHDADGERLGLITEACEILPEEATLAVACEIELARAPGMV
                     VTNVSTSAAIDAIAARHGGAVLRTPVGQAFVSEAMIETGAVVGGEGSGGVVVPRVQLT
                     HDSAAAVGLILEHMALTGERISEVAARLPRFTMLKHNVAVEPNRIHSLVQRLHEELVR
                     EGVAYEQTDGIKISWPDGWVHVRVSNTESLVRVIAEAEDPKRAGELLDWARDRVGR"
     gene            complement(1660..4236)
                     /locus_tag="AC159P_00085"
     CDS             complement(1660..4236)
                     /locus_tag="AC159P_00085"
                     /EC_number="3.2.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015253531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004567 - beta-mannosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005537 - D-mannose binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-mannosidase"
                     /protein_id="HIR3693876.1"
                     /translation="MIIFAAAACHAQTPSAAGWPGQRASVPLHGGWKFREAGKGAWRA
                     AKVPGCVHTDLLKNGLIEDPFYRDNEKRLQWIGKTDWEYETTFRVSAEMLARRNVELV
                     FEGLDTYAAVALNGVELLSADNMFRTWRVDAKKALKPGENTLRVVFRSPINEVLPRMK
                     ALGYELPASNDQGEKTSPHTRKAPYHYGWDWGPRFVTSGIWKPVRLEAWDGARVADFH
                     VEQKQLDRAAATMTAVVELVADRDTDATVILDEASGSRRLAARSVKLRPGVNRVRVDF
                     RLERPRLWWPAGLGPQHLYSFRTLVLDGKQLLDEAATRVGLRTLELRQQPDQWGKSFE
                     FVVNGVPVFAKGGNWIPADSFPVRVTRERYRQLLESARDANMNMLRVWGGGIYELDDF
                     YELCDELGLLVWQDFMFACSMYPGDKDFLESVRAEAVDNVRRLRNHPSIVLWCGNNEV
                     ETAWLHWGWKQNLPAKLWDDYKKLFHGVLPEVVAEHDPSRPYWPSSPSSNLEEDPDSQ
                     RMGDVHYWEVWHASKPFEFYEKQHPRFMSEYGFQSFPLIESVNAYTVPADHDIQSPVM
                     LVHQKHPRGNQLIREYMLREYAEPKDFESFLYVSQFLQAEGIRVGTEHFRRIRPRNMG
                     ALYWQVNDCWPVASWSGMDYFGRWKALHYHARRFFRDLLVSPHEEDGRIRIHVVSDRT
                     TPTPATLRVALMDFAGRVLFEKREDVRIQPLSSSVQLSLDKSDLLRGHDPRAVFLDCE
                     LTEKNPTTARGAATESGAAAPGNRLFFAPFKDLRMEAPLIDTQVRPAGRSLSVRLRTD
                     KLARGVHLSAGTLDGAFRDNFFDLLPGREVTVEFRPRRRVTVAELSRALKVRSLADAF
                     NK"
     gene            4417..6606
                     /locus_tag="AC159P_00090"
     CDS             4417..6606
                     /locus_tag="AC159P_00090"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008104469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004563 - beta-N-acetylhexosaminidase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HIR3693877.1"
                     /translation="MNARKSVPAILTSFILTSTILTPLASASARTAPRPAAAQDAAQA
                     RRHQLMPVPASVRFGAGRLPITKSFTVAAKGHVDERLRAGIDRAVRRLEGRTVLELAR
                     GLSEDAAAATLVVECRGPGRAVPSVDEDESYTLEVTERQAALSAPTVVGALRGLETFL
                     QLLEGDRAGHFIPAVSIQDKPRFPWRGLLMDVARHWQPVEVVKRNLDGMAAVKLNVLH
                     WHLTEDQGFRVESKKLPRLHQMGSDGNFYTQEQIRDVIAYAAARGIRVVPEFDLPGHA
                     TAWLVGHPELGSAPGPYTIERRPGIFEPALDPTREEVYKFLDTFMGEMAALFPDAYLH
                     IGGDENEGKQWDRNPKIQAFMKEKGIKDNHALQTYFNQRLLQTLRKHNKRMMGWDEIF
                     QPDLPKDVVIHSWRGQTALAEAARKGYEGVLSNGYYIDLIFPTHQHYLNDPLPAGSTL
                     TPTEAARVLGGEATMWSEWVTPETIDSRIWPRTAAIAERLWSPREVVDVEDMYRRLAT
                     TSVRLEELGLLHERNQMMMLRRLAQGRDVAPLHTLVSIVEPVKEYRRYQQRPQTMLSP
                     LTGLVDAARPDSDAARRFHALVEALLSDAPRFEVHSEELRRTLTAWRDAGTELEAMID
                     ASPALHEARPLAADLASLGSAGLEALSYLSRGVAPPAEWRDARLASVEQAAKPKAALE
                     FVVVTGVRQLVVAAAEQPRLREATASEWKSHVKAMAAPPANQPAKQP"
     gene            6845..7699
                     /locus_tag="AC159P_00095"
     CDS             6845..7699
                     /locus_tag="AC159P_00095"
                     /EC_number="3.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014265193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HIR3693878.1"
                     /translation="MKLLLTLLLLAAPVAAQELPSRWDELTAGDWPKAQERAAYTCIL
                     PIGILEKHGPHVPLGSDLIMAREWSARAARQEYVVVFPDFFYGQIYEAKHQPGTFALP
                     SRLVWELLDATVEEIARNGFKKIVIVNGHGGNPQLLRYFVQSQLERRRDYVVYFFDPA
                     YDAKAEEEKFKPLRKTDPSIDLHAGETETSVLLYLRPDLVQMPRAAEESGADQNRLAL
                     PDLYTAIWWYARFPNHYSGEGAKGSRELGQALTEHRVATLVKALRVVKTDDKSLKIQS
                     EFFDRIKK"
     gene            complement(7713..9506)
                     /locus_tag="AC159P_00100"
     CDS             complement(7713..9506)
                     /locus_tag="AC159P_00100"
                     /inference="COORDINATES: protein motif:HMM:NF024907.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HIR3693879.1"
                     /translation="MSKSPPRLSRLIVGALLLDCVIAVSSSSFVSVAAQPSAPVKAPA
                     ITFADISATSGLNVALVSTPEKRYIIESMSGGVALFDCDADGRLDVATVNGSSVERFK
                     AGGDPFVTLYRQANDARSHASTPLKFENVTTAAGLARKGWGMGVTAVDYDDDGTLDLF
                     VTGYEGNVLYRGTGPCRFQDVTEKAGLGGGGFATGPAWADYDRDGDLDLFVARYVHVD
                     LDNLPEFGGSAKTCTYRGIRVQCGPRGLPGESDLFYRNRGDGTFEEVARKAGVADERK
                     HYGMAGIWGDYDDDGWADLFVANDATYNYLYRNQRDGTFKETGAVAGVAYGESGAEQG
                     SMGVTWGDFDHDGRLDLFVSNFENEPNALYKNLGATGFVDVTRTSKIGHASLPYVGWG
                     TAFTDFDNDGFADLLIANGHVYPQIELAQGKYPTGYRQHFLLFRNLGTGAFEEVSEAA
                     GLRGLPLRSRRGAAFGDLDDDGLVDAVVVNLAEPPTVLLNTSDDRNQRVTLKLVQTKG
                     NRDAVGARVTLRTPKRTQLQEVQAGASYLSQNDLRLHFGLGQGEKIERVEVRWSGGET
                     ETVTGVTPDRIVTVTQGKGVTASVAYQPRRR"
     gene            complement(9753..11489)
                     /locus_tag="AC159P_00105"
     CDS             complement(9753..11489)
                     /locus_tag="AC159P_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HIR3693880.1"
                     /translation="MKTALIFIFLLMCGASPRVAAQPKSADCGARKIPQLVDVTAKTG
                     IRFAHTSAPEKKYIVESMSGGVLLVDYDRDGWQDIYFTNAPTVEMALKGQRARSALYR
                     NNGDGTFADVTDKAGVAYPGFAMGGAVTDYDNDGWPDIYVTCLGGNVLYRNNGDGTFA
                     DVTKKAGVGDGRWSAGAAFGDYDGDGHLDLMVANYVDFKLDDLPGFGSSPTCKFRGID
                     VQCGPRGLKGAGDALYRNNGDGTFRDVTRESGMADPGGYYGMQVVWADFGGRGRLDAF
                     VANDSTPNFFYKHEGGNKFAEIGLESGTAVSGDGSEQGCMGVAVGDYLRNGRFSVFVT
                     NFADEYNTLYRNDGDYSFTDVSFAAGVAQISRPYVGWGTSFFDLDNDGWLDLLVVNGH
                     VYPQIDTLPSGARYRQPKLLHLNLGGGAFCDASRQAGAALQEARASRGAAFGDLDNDG
                     DIDVVVGDIDGPPMVLRNEGGDGNQWITFELAATKGNPLAIGARVRVVTGDVVQTEEI
                     RSGTSYLSQNDLRLHFGLGKHAKVDLLEIRWPSGKVEQVKDLAAGKFYAVREGEGVVP
                     AERARPAAKAKK"
     gene            complement(11644..13197)
                     /locus_tag="AC159P_00110"
     CDS             complement(11644..13197)
                     /locus_tag="AC159P_00110"
                     /inference="COORDINATES: protein motif:HMM:NF024100.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3693881.1"
                     /translation="MSRRRERRARPLPLCLTLLVACALQHVAAQPRTGAGDAALARIE
                     QSIAQGRLEEAERPLLDYAVAHPKDPRALELLGRLRRLQGRSEEAEALYRRVLALDSS
                     RTQAKLNLAHLVHEAGRPDEARALVADAARTPPTDPRARLALAEALALVGEHRQALAV
                     AETLPSALKNTEALPVVAAALQALGEGGKLKALVPSMRRAAVARPGVAARCAGVLREA
                     GMKEQAVALLRAALASAPDDAGVLVSLGRLEVEARDFEQARRHLERAASVAPRSAEAL
                     AALAALEGARGDAAAALVALEKARALSPDSAPLLAQLVVAAMRANKPGAAVEAASTLL
                     RLKPDEPEYLYLHGAALLQGGSMRAAQEALERYARARPRDSRGCLALGLALAGQHDRF
                     DAARAQLERCLDLDPSNTEAKYQLGLAHRAQGEAAKAVKFLEEVVAAAPEHAGALRDL
                     GALYAQAGESSKARAALERAATLDPLDAETHFQLSRLYSRLGEAALAQRHLEQFQNLK
                     SQREKVPAP"
     gene            complement(13187..15616)
                     /locus_tag="AC159P_00115"
     CDS             complement(13187..15616)
                     /locus_tag="AC159P_00115"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3693882.1"
                     /translation="MSKFLSLGLLSLSLWLPAAGAAQQQQPQPQQRPQQQPQPQQPAG
                     KPNPAQRPQPDQLERHLGAAETYQLSGDIARAAAENRAVVAVALGHLGALAIRERQLQ
                     RAARLLSDSLSLREDARVRTDLAIAHMRLMEIDRAVAEAQAAAASDERDARARHVLGK
                     LLYMKGDYAGGRRELERAVVLGTDLDAAYTLGMTYLRLKELDRAKLLFEEMQTALGDS
                     AVAHILFGRAYEETGFPAEAEREFKRALAIDPAAPRAHFYLGYVILMNGGGERVAEAA
                     AEFERQLQKNPRDFYAHFFLGLIAAGESDHRKAVKHLAEAVRLKPESGESYLHLGQSQ
                     AELGDPAAEQSLRRAIVLTADVSQNSYQIKRAHFLLGRLLMKAGRKAEGEKELALARE
                     LQGQSLESSRQRIKEILGSVAKSAGGVSAPSQAAAAGEKKSDEADEAEVSVVEESPVE
                     SRDEQEVLLIEESAPDSRDAARYASLKLRLGEVLAQAYHNLGVIAAQQGRPAEGAAQF
                     AAAAAWKPDLAGLDRNWGIVSFSAGQHEQAVQPLSRHLLKQPADTLARRMLGVSYYLT
                     GNFKQAVETLRPLDAVITDDAELAYAYGVSLVRLADAKAATALFTRLAERHARAPQAR
                     LYAAQGFMMIADYDGALREYRAAARLDPALRQAHYNAGLSLIHLNRLDEAEQEFRQEL
                     ALNPADEVSKYHLAYVLLESKQKTAEAVTLLKEAIAARPDYADARYRLGKALSEQGDV
                     AGAIEHLEAAARAEPAKDYIRYQLSIAYRRAARPADAERELQLYKDLKASSRSREAPQ
                     PGAMGDKPNVP"
     gene            complement(15735..>16063)
                     /locus_tag="AC159P_00120"
     CDS             complement(15735..>16063)
                     /locus_tag="AC159P_00120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3693883.1"
                     /translation="TPGWIRKPFGEGARVQFRLELFNAFNHTQFRGDSLQNNMNLAGG
                     NVNCAGMPTTFNCSATNRVITQGLKPADAPNPTVPIPVPVNGNFGRSGTTRGPREIQY
                     ALKLVF"
BASE COUNT         2486 a         5432 c         5513 g         2632 t
ORIGIN      
        1 aacgccgggc aagcacatcg cgtacgcggt acgtcgcgtc ggcgacgtgt gaacccgttc
       61 agacgtcgct gacgcgacgc ggcgtccttc ctgtgtgcct aacccggcct tgaaaggccg
      121 ggctacactc aaccgccgct acgcggcgaa caaggcgcct acgaatacat tccgtcttcg
      181 acttcttacc tacgcctttc tgtccattca ccgcccgacg cggtcgcgcg cccagtcgag
      241 cagttcgccc gcgcgcttgg ggtcttcggc ctcggcgatg acgcggacga gcgattcggt
      301 gttggagaca cggacgtgaa cccagccgtc gggccacgaa atcttgatgc cgtcggtctg
      361 ttcgtaggcg acgccctcgc gcaccagctc ttcgtgcagg cgctgcacga gcgagtggat
      421 gcggttcggc tcgacggcga cgttgtgttt gagcatcgtg aagcgcggca ggcgcgcggc
      481 gacttcggag atgcgctcgc cggtcagggc catgtgttcg aggatgaggc cgaccgccgc
      541 cgcgctgtcg tgcgtgagct ggacgcgcgg gacgacgacg ccgccgctgc cctcgccgcc
      601 gacgacggcg ccggtctcga tcatcgcctc ggagacgaac gcctgcccga cgggcgtgcg
      661 caacaccgcg ccgccgtggc gcgcggctat cgcgtcgatg gccgcgctgg tcgagacgtt
      721 ggtgacgacc atgcccggcg cgcgcgccag ctcgatctcg caggcgacgg cgagcgtcgc
      781 ctcctcgggc agtatctcgc acgcctcggt gatgaggccg aggcgctcgc cgtcggcgtc
      841 gtgcgcgaag ccgatgtcgg cgcggcccgc cttcacgacg gcgcgcagtt gcgccatcgt
      901 ctgccgccgc ggctcgggcg cgtgcgggaa aggcatggcg gggtcgtcgt tcacggcgaa
      961 gacttcgcag ccgagcgagg cgagccagcg cggcgacagc agcgcgcacg agctgttgca
     1021 gcaatcgacc gcgaccgtca ggcggcgcga cctgatcgcc tccacgtcga aggcgtccga
     1081 caacgtttcg aggtggtgcg ggatggcgtc cgcgcgctcg acgcggctgt ggatgtcggc
     1141 ccacgtcgct ttggcgaact cggcctgatg gtagatgtcc aaaagctcgt cggcctgcgt
     1201 cgcgttcagg tagaggccgt ccgagcggac gaacttgagc gcgttccagg gttccgggtt
     1261 gtggccggcg gtgatggaaa tgccgccgac ggcgcccagg cggcggacgc cgagttgcag
     1321 gctcggcgtc gggcagatgc cgaggtcgaa gacctcgcag cccgcggcga gcaggcccgc
     1381 gagcaccgcg gcgcgcacca tcgggccgga cgcgcgcgcg tcgcggcaga cgaggatgcg
     1441 ccccgaatcg aggtaggtgc cgaaggcttg cgcgaagccg acggccagct cgggcgtgaa
     1501 ggtctcgccg acgacgccgc gcacaccgct gatgccgatc ttgagcgagt tcatcagaag
     1561 aaataatgat gaatgcggaa tgcggaatga tgaatcaaag aaaagccgaa gggtgacatt
     1621 cagcgtgctt cttattcatc attccgcatt ccgcattcat catttattga aggcgtcggc
     1681 gagcgagcgc accttcaacg cgcgagacag ctcggccacg gtgacgcggc ggcgcgggcg
     1741 gaactcgacc gtgacttccc tgcccggcag gaggtcgaag aagttgtcgc ggaaggcgcc
     1801 gtcgagcgtc ccggcggaga gatggacgcc gcgcgcgagt ttgtccgtgc gcagccggac
     1861 gctgagcgag cggcccgccg gccggacttg cgtgtcgatc agcggcgcct ccatccgcag
     1921 gtctttgaag ggcgcgaaga agagacgatt gcccggcgca gccgcgccgc tctcggtcgc
     1981 cgcgccgcgc gccgtcgtcg ggtttttctc cgtcagctcg cagtcgagga agaccgcacg
     2041 cgggtcgtgg cccctcaaca ggtcgctttt gtcgaggctc aattggacgg agcttgagag
     2101 cggttgtatt cgaacgtcct cgcgcttctc gaagagcacg cggcccgcga agtccatgag
     2161 cgcgacgcgg agcgtggcgg gcgtcggggt ggtgcggtcg gagacgacgt ggatgcggat
     2221 gcgcccgtcc tcttcgtgcg ggctgacgag gaggtcgcgg aagaagcggc gggcgtggta
     2281 gtggagcgcc ttccagcgcc cgaagtagtc catccccgac caggaggcga cgggccagca
     2341 gtcgttgacc tgccagtaga gcgcgcccat gttgcgcggg cggatgcggc ggaagtgctc
     2401 tgtgccgacg cggatgccct cggcctgcaa gaactggctg acgtagagga acgattcgaa
     2461 gtccttcggc tcggcgtact cgcggagcat gtactcgcgg atgagctggt tgccgcgcgg
     2521 gtgcttctgg tgcacgagca tgacgggcga ctggatgtcg tggtcggcgg ggacggtgta
     2581 ggcgttgacc gactcgatga gcgggaacga ctggaagccg tattcgctca tgaagcgcgg
     2641 gtgctgcttc tcgtaaaact cgaagggctt cgaggcgtgc cagacttccc agtagtgcac
     2701 gtcgcccatc cgctgcgagt cggggtcttc ttccagattc gaactcggcg aactcggcca
     2761 gtacgggcgc gaggggtcgt gctcggcgac cacctcgggc agcacgccgt ggaacagctt
     2821 cttgtagtcg tcccacagct tcgcgggcag gttctgcttc cagccccagt ggagccaggc
     2881 ggtctcgacc tcgttgttgc cgcaccagag gacgatgctc ggatgattcc tcaaacgcct
     2941 gacgttatcg accgcttcgg cgcggacgct ctccagaaaa tctttgtcgc ccggatacat
     3001 gctgcacgcg aacatgaagt cctgccagac caacaggccc agctcgtcgc acagctcgta
     3061 gaaatcgtcc agctcgtaga tgccgccgcc ccagacgcgc agcatgttca tgttcgcgtc
     3121 gcgcgccgat tcgagcagtt ggcggtagcg ctcccgcgtc acgcgcacgg ggaagctgtc
     3181 cgccggaatc cagttgccgc ccttggcgaa gacgggcacg ccgttgacga cgaactcgaa
     3241 gctcttgccc cactgatccg gctgctgccg cagctccagc gtgcgcaggc cgaccctcgt
     3301 cgcggcctcg tccaacagtt gcttcccgtc aaggacgagc gtgcggaagc tgtagaggtg
     3361 ctgcggcccg agacccgcgg gccaccagag gcgcgggcgt tcgagacgaa agtctacccg
     3421 gacgcggttc acgccgggac gcagcttgac cgaacgcgcg gccagccgcc tgctgcccga
     3481 cgcctcgtcg aggatgacgg tcgcgtccgt gtcgcggtcg gcgacgagtt cgacgacggc
     3541 ggtcatcgtc gccgccgcgc ggtcgagttg cttctgctcg acgtggaagt ccgcgacgcg
     3601 cgcgccgtcc cacgcttcga gcctgaccgg cttccagatg ccgctcgtca cgaagcgcgg
     3661 cccccagtcc cagccgtaat ggtacggcgc cttgcgcgtg tgcgggctgg ttttctcgcc
     3721 ctgatcgttc gacgcaggga gttcataccc gagcgccttc atccgcggca gcacctcgtt
     3781 gatcggcgag cggaaaacga cccgcaaagt gttctcgccc ggcttcagag ccttcttcgc
     3841 gtccacgcgc cacgtgcgga acatgttgtc ggcggagagc aattcgaccc cgttgagcgc
     3901 gaccgcggcg tacgtgtcga gaccctcgaa gaccagctcg acgttgcggc gggcgagcat
     3961 ttccgccgag acgcggaagg tcgtctcgta ctcccagtcg gtcttgccga tccattgcaa
     4021 acgcttctcg ttgtcgcggt aaaatgggtc ttcgatcagc ccgttcttca acaggtctgt
     4081 gtggacgcag ccgggcacct tggccgcgcg ccacgcgccc ttgcccgcct cgcggaactt
     4141 ccagccgccg tgcaggggga cggacgcgcg ctgccccggc cagcccgccg ccgacggcgt
     4201 ctgcgcgtgg caggcggcag ccgcgaagat aatcatacag agtgctctgg agacgaagat
     4261 tcgcatgcag cctttacacc tacttttacc ggtattaaat tctgtaactc aagtatgaca
     4321 ccgtaactta cgccgcgcga gcctgttctt cggcgaaact caagttacta ttccgacgcc
     4381 gtacgaacca acacacccga ccaatcagag gagcacatga acgcgagaaa atccgtcccg
     4441 gccatcctga cttcgttcat cctgacttcg accatcctga caccgctcgc gtccgcgtcc
     4501 gcgcggacgg ccccgcgccc cgccgccgcg caggacgccg cgcaggcacg gcgccaccaa
     4561 ctgatgcccg tgcccgcctc ggtccgcttc ggcgcgggcc gactgcccat caccaaatcg
     4621 ttcacggtcg ccgcgaaggg gcacgtggac gagcgcctgc gcgccgggat agaccgcgcc
     4681 gtccgccgtc tcgaagggcg cacggtcttg gagcttgcgc gcggcctctc ggaggacgcc
     4741 gcggcggcca cgctcgtcgt cgagtgccgc gggccgggcc gggccgtccc ctcggtcgat
     4801 gaggacgaat cgtacaccct cgaagtcacg gagcgtcagg ccgccttgag cgcgcccacg
     4861 gtggtcggcg cgctgcgcgg gctggagacg ttcctccaac tcctcgaagg cgaccgcgcg
     4921 ggccacttca tcccggcggt cagcatacag gacaagccgc gctttccgtg gcgcgggctg
     4981 ctcatggacg tggcgcgcca ctggcagccc gtcgaggtcg tcaagcgcaa cctcgacggc
     5041 atggccgccg tcaagctcaa cgtgctgcac tggcacctca cggaagacca gggcttccgc
     5101 gtcgagagca aaaaacttcc gcgcctccat cagatgggtt ccgacggcaa cttctacacg
     5161 caggagcaga tccgcgacgt gatcgcctac gccgccgcgc gcggcatccg cgtcgtgccg
     5221 gagttcgacc tgccgggcca cgcgaccgct tggctcgtcg gccaccccga actcgggagc
     5281 gcgccggggc cgtacacgat cgagcgccgc cccggcatct tcgaacccgc gctcgacccg
     5341 acgcgcgaag aggtctacaa gttcctcgac accttcatgg gcgagatggc cgcgctcttc
     5401 cccgatgcgt atcttcacat cggcggcgac gagaacgaag gcaagcagtg ggacagaaac
     5461 ccgaagatac aagccttcat gaaggagaaa ggcatcaagg acaaccacgc gctccagacc
     5521 tacttcaacc agcggctcct gcaaaccctc aggaagcaca acaagcgcat gatgggctgg
     5581 gacgaaatct tccagcccga cctgccgaag gatgtcgtca tccactcctg gcgcggtcag
     5641 accgcgctcg ccgaggccgc gcgcaagggc tatgaaggcg tcctctcgaa cggctactac
     5701 atcgacctca tcttccccac gcaccagcac tatcttaacg acccgctgcc cgccggcagc
     5761 acgttaacac cgacggaggc cgcacgcgtc ctcggcggcg aggcgacgat gtggagcgag
     5821 tgggtgacgc ccgagaccat cgactcgcgc atctggccgc gcaccgccgc catcgccgaa
     5881 cgcctctggt cgccgcgcga ggtcgtggac gtggaggaca tgtaccgccg cctcgccacg
     5941 acgagcgtcc gcctcgaaga actcggcctc ctgcacgagc gcaaccagat gatgatgctc
     6001 cgccgtctcg cgcaagggcg cgacgtggcg ccgctccaca cgctcgtctc catcgtcgag
     6061 ccggtgaagg agtaccgccg ctaccaacag agaccgcaga cgatgctctc gccgctgacg
     6121 ggactcgtgg acgcggcccg ccccgatagc gatgcggccc gccgcttcca cgcgctcgtc
     6181 gaagcgctgc tctccgacgc gccgcgcttc gaagtgcact cggaagaact ccgccgcacg
     6241 ttgaccgcgt ggcgcgacgc gggcaccgaa ctcgaagcga tgatcgacgc ctcgcccgcg
     6301 ctgcacgaag cgaggccgct cgccgccgac ctcgcctcgt taggctccgc gggcctcgaa
     6361 gccctctcgt atctttcgcg cggcgtcgcc ccgcccgccg agtggcgcga cgcgcgcctg
     6421 gcctccgtcg agcaggccgc gaagccgaag gccgccctcg aattcgtggt cgtcacgggc
     6481 gtccgccaac tcgtcgtcgc cgccgccgaa cagccgcggc tcagagaggc gacggcctcc
     6541 gaatggaagt cgcacgtcaa ggcgatggcc gcgccgcccg cgaaccagcc cgcgaagcag
     6601 ccttgagtcg cgggtcggcg ccaccaccta caacatcccc atcccgatgc tggcgctccg
     6661 ccagcagtga aagggggtgt tgggtgttag gtgttgggtt ttggatcgaa cagcacgcaa
     6721 cgtgcggtca gcagccgtgt cccggtttcg gcttagccga agccgaccac ggacgactga
     6781 actctcctcc aatccaaaac ccaacaccta acacccaaca cccgaaaacc ctctggagac
     6841 tcgtatgaaa ctgctgctca ccttgctgct cctggccgcg ccggtcgcgg cgcaggagct
     6901 gccgtcgcgc tgggacgagc tgacggcggg cgactggccg aaggcgcagg agcgcgccgc
     6961 ctacacgtgc atcctgccca tcggcatcct cgaaaagcac ggcccgcacg tcccgctcgg
     7021 ctccgacctg atcatggcgc gcgagtggtc tgcgcgcgcc gcacggcagg agtacgtcgt
     7081 cgtcttcccc gactttttct acgggcagat ttacgaggcg aagcaccagc cgggcacctt
     7141 cgctctgccg tcgcggctgg tgtgggagct gctcgacgcg accgtggagg agatcgcgcg
     7201 caacggcttc aagaagatcg tcatcgtcaa cggccacggc ggcaacccgc aactgctgcg
     7261 ctacttcgtc cagtcgcagc tcgaacgccg ccgcgactac gtcgtctact tcttcgaccc
     7321 cgcctacgac gccaaagccg aggaggagaa gttcaagccg ctccgcaaga cagacccgtc
     7381 catcgatctg cacgcgggcg agaccgagac ctccgtgctc ctctacctgc gccccgacct
     7441 cgtgcagatg ccgcgcgccg ccgaagagtc cggcgcagac caaaaccgcc tcgcgctgcc
     7501 cgacctctac accgccatct ggtggtacgc gcgcttcccc aaccactact ccggcgaggg
     7561 cgccaaaggc tcgcgcgaac tcgggcaggc gctcaccgag caccgcgtcg ccacgctcgt
     7621 caaagccctg cgcgtcgtca agaccgacga caagtcgctc aagattcaaa gcgagttctt
     7681 cgaccgcatc aagaagtgag tcggcgtgct cgtcatctgc gccgcggctg atacgcgacc
     7741 gaggcggtga cgcccttgcc ctgcgtgacg gtcacgatgc ggtcgggcgt gacgcctgtg
     7801 acggtctcgg tctcgccgcc gctccagcgc acctcgacgc gctcgatctt ctcgccctgc
     7861 ccgaggccga agtgcaggcg caggtcgttc tgcgaaaggt agctcgcgcc cgcctgcacc
     7921 tcctgtagct gcgtgcgctt cggcgtgcgc aacgtgacgc gcgcgccgac ggcgtcgcgg
     7981 ttgcccttcg tctggacgag cttgagcgtg acgcgctggt tgcggtcgtc gctcgtgttc
     8041 aagagcacgg tcggcggctc ggcgaggttg accactacgg cgtccacgag tccgtcgtcg
     8101 tcgaggtcgc cgaaggccgc gccgcgccgc gagcgcaggg gcaggccgcg caggcccgcc
     8161 gcctccgaca cctcttcgaa ggcgcccgtg ccgaggttgc ggaagagcag gaagtgctgg
     8221 cggtagccgg tcgggtactt cccttgcgcc agctcgatct gcgggtagac gtggccgttg
     8281 gcgatgagca ggtctgcgaa gccgtcgttg tcgaagtccg tgaaggccgt gccccagccg
     8341 acgtaaggca ggctcgcgtg gccgatcttc gacgtgcgcg tcacgtccac gaagccggtc
     8401 gcgccgaggt tcttgtagag cgcgttcggc tcgttctcga agttgctgac gaagaggtcg
     8461 agcctgccgt catggtcgaa gtcgccccac gtcacgccca tcgacccctg ctcggcgccc
     8521 gactcgccgt aggccacgcc cgccaccgcg cccgtctcct tgaaagtccc gtcgcgctgg
     8581 ttgcggtaga ggtagttgta ggtggcgtcg ttggcgacga agaggtctgc ccacccatcg
     8641 tcgtcgtagt cgccccagat gcccgccatg ccgtagtgct tgcgctcgtc cgcgacgccc
     8701 gccttgcgcg cgacctcttc gaacgtgccg tcgccgcggt tgcggtagaa gaggtccgac
     8761 tcgccgggca gcccgcgcgg gccgcactgg acgcggatgc cgcggtaggt gcaggtcttg
     8821 gcggagccgc cgaactcggg caggttgtcg agatcgacgt ggacgtagcg cgcgacgaag
     8881 aggtcgaggt cgccgtcgcg gtcgtagtcg gcccatgcag ggccggtggc gaaaccgccg
     8941 ccgccgaggc ccgccttctc cgtcacgtcc tgaaacctgc acggccccgt gccgcgatag
     9001 agcacgttgc cctcgtagcc tgtgacgaag aggtcgagcg tgccgtcgtc gtcgtaatcg
     9061 accgcggtca cgcccatgcc ccaacccttg cgcgcgagtc ccgccgccgt cgtcacgttc
     9121 tcgaacttga gaggcgtgct ggcatgactc cgcgcgtcgt tcgcctgtcg atagagcgtg
     9181 acgaacgggt cgccgcccgc cttgaagcgc tcgaccgaag agccgttcac cgtcgccacg
     9241 tccaggcgcc cgtccgcgtc gcagtcgaac agcgcgacgc cgccgctcat cgactcgatg
     9301 atgtaacgct tctccggcgt cgagacgagc gcgacgttga ggccgctcgt cgccgagatg
     9361 tcggcgaacg tgatggcggg agccttaacg ggagcagaag gctgagcggc gactgagacg
     9421 aagcttgacg aagagaccgc gatgacgcag tcgagcagaa gcgcgccgac gataaggcgt
     9481 gagagacggg gcgggctttt gctcatagga actcttgttg aagtatctgc ctgcggctgt
     9541 gccgcctgat gtgcggtgag gcttcggcca caccatgagc gccgccaaac aaagcgcgga
     9601 ggctacgcct ccgcgcgggg cgtagcccca aaagattttt agagcgcgtc ttgcggaagg
     9661 gctgtgccct tccgcacatc aaggggcaca gccgccatgc ctgtgagaca tccggcgata
     9721 cagccgccat gcctgcgact cacgcacccg gcctatttct tcgccttcgc ggcggggcgg
     9781 gcgcgctcgg cagggacgac gccttcgcct tcgcgcacgg cgtagaactt gccggcggcg
     9841 aggtctttga cctgctccac cttgcccgag ggccagcgga tttccagcag gtctaccttc
     9901 gcgtgtttgc cgaggccgaa gtgcagacgc aggtcgttct gcgaaaggta gctcgtgccg
     9961 ctgcgtatct cttcggtctg cacgacgtcg ccggtgacga cgcggacgcg cgcgccgatg
    10021 gcgagcggat tgcctttcgt cgccgccagc tcgaaggtga tccactgatt gccgtcgccg
    10081 ccctcgttgc gcaagaccat cggcggcccg tcgatgtcgc ccacgaccac gtcgatgtcg
    10141 ccgtcgttgt cgaggtcgcc gaaggccgcg ccgcgcgagg cgcgcgcctc ttgcagtgcc
    10201 gcccctgcct gacggctcgc gtcgcagaag gcgccgccgc cgaggttcag gtgcagcagc
    10261 ttcggctggc ggtagcgcgc gcccgaaggg agcgtgtcga tctgcgggta gacgtggccg
    10321 ttgacgacga ggaggtcgag ccagccgtcg ttgtcgaggt cgaagaagct cgtgccccag
    10381 ccgacgtagg ggcgcgaaat ttgcgcgacg ccggcggcga aggaaacgtc ggtgaagctg
    10441 tagtcgccgt cgttgcggta gagcgtgttg tactcgtcgg cgaagttggt gacgaagacc
    10501 gagaagcgcc cgttgcgcaa ataatccccg acggcgacgc ccatgcagcc ctgctccgag
    10561 ccgtcgccgc tgacggcggt gccggattcg agaccgatct cggcgaactt gttgcccccc
    10621 tcgtgcttgt aaaaaaagtt cggcgtcgag tcgttggcga cgaaggcgtc gagccgcccg
    10681 cgtccgccga agtcggccca cacgacctgc atcccgtagt agccgccggg gtctgccatg
    10741 ccggactctc tggtgacatc cctgaacgtg ccgtcgccgt tgttgcggta gagcgcgtcg
    10801 cccgcgccct tcaggccgcg cggcccgcac tgcacgtcga tgccgcggaa cttgcacgtc
    10861 gggctcgagc cgaagccggg caggtcgtcg agcttgaagt ccacgtagtt ggcgaccatc
    10921 aaatcgagat ggccgtcgcc gtcgtagtca ccgaacgccg cgcccgcaga ccagcgaccg
    10981 tccccgacgc ccgccttctt cgtcacgtcc gcgaacgtgc cgtcgccgtt gttgcggtag
    11041 agcacgttgc cgccgaggca ggtcacgtag atgtcgggcc agccgtcgtt gtcgtagtcg
    11101 gtgacggcgc cgcccatcgc gaagccgggg taggcgacgc ccgccttgtc cgtcacgtcc
    11161 gcgaaggtgc cgtcgccgtt gttgcgataa agcgcgctgc gcgcccgctg gcctttgagc
    11221 gccatctcga ccgtgggcgc gttcgtgaag tagatgtcct gccagccgtc gcggtcgtaa
    11281 tcgacgagca gtacgccgcc gctcatcgac tcgacgatgt acttcttctc gggcgccgag
    11341 gtgtgcgcga agcggatgcc ggtcttcgcc gtcacgtcca caagctgcgg gattttacgc
    11401 gcgccgcagt cggcggactt cggctgcgcg gcgacgcgcg gcgacgcgcc acacatcagc
    11461 aagaagataa agatgagggc agttttcata ttggtgcgcg gcgtccgcca gagctgtagg
    11521 ggcagggctt gtcccgcccc gctgtcgata ttcgagttgc gtattaccgc gcgagcgggc
    11581 agggacaagc cctgccccta caaccatcac gggcgttccg cgcccaccgt gggaagctcg
    11641 tcctcatggc gcgggcactt tctccctttg gctcttcagg ttttggaact gctcaaggtg
    11701 tctctgcgcg agcgcggcct cgccgaggcg gctgtagagg cggctgagtt gaaagtgcgt
    11761 ctcggcgtcc agcgggtcga gcgtggccgc gcgctccaga gcggcgcgcg ccttcgacga
    11821 ttcgcccgcc tgcgcgtaga gcgcgccgag gtcgcgcagc gcgcccgcgt gctcgggcgc
    11881 ggcggcgacc acctcttcga gaaacttgac ggccttcgcg gcctcgccct gcgccctgtg
    11941 ggcgaggccg agctggtact tcgcctctgt gttcgagggg tcgagatcga ggcagcgttc
    12001 gagctgcgcg cgcgccgcgt cgaaccggtc gtgctggccg gcgagcgcga ggccgagcgc
    12061 gaggcagccg cgcgaatccc gcggtcgcgc gcgcgcgtaa cgctcaagcg cctcttgcgc
    12121 cgcgcgcatg ctcccgccct gcaacagcgc cgcgccgtgg aggtagaggt attcaggctc
    12181 gtccggcttc agccgcaaga gcgtgctcgc cgcctccacc gccgcgccgg gcttgttcgc
    12241 gcgcatcgcc gcgacgacaa gctgcgcgag cagcggcgcc gagtcgggag acagagcacg
    12301 cgccttctcc agagcgacga gcgccgccgc cgcgtcgccc cgcgcgcctt cgagcgcggc
    12361 cagcgcggcg agcgcttccg ccgaacgcgg cgcgaccgaa gcggcgcgtt cgaggtgcct
    12421 tcgcgcctgc tcgaagtcac gcgcctcgac ttcgagccga ccgagcgaga cgagcacgcc
    12481 cgcatcgtcc ggggcggacg cgagcgccgc acgcagcagc gcgacggcct gctccttcat
    12541 ccccgcttcg cgcaacaccc cggcgcaccg cgcggccacg ccgggccttg cgacggcggc
    12601 gcggcgcatc gaagggacga gcgccttcag cttcccccct tcgccgagcg cttgcagagc
    12661 ggcggcgacg acgggcagcg cctccgtgtt tttcaacgcg gacggcagcg tctctgcgac
    12721 ggcgagcgcc tgccggtgct cgccgacgag cgcgagcgct tcggcgagcg cgagtcttgc
    12781 gcgcgggtct gtgggcggag tgcgcgcggc atcagcgacg agggcgcggg cctcgtccgg
    12841 tcggcccgct tcgtggacga ggtgcgcgag attcagcttc gcctgtgtgc gggatgaatc
    12901 gagcgcgagg acgcggcggt ataaagcctc tgcctcttcg gagcggcctt gcaggcggcg
    12961 cagtcgcccg agcaattcca acgcgcgcgg gtctttcggg tgtgcgacgg cgtagtcgag
    13021 caaagggcgt tcggcctctt cgagtcggcc ctgcgcgatg ctctgctcga tgcgcgcgag
    13081 ggcggcgtct ccggcgcccg tgcggggctg tgccgcgacg tgttggaggg cgcaggcgac
    13141 gagcagtgtg aggcagagcg gcaggggccg cgcccgccgt tcgcgcctac gggacattcg
    13201 gcttgtcccc catcgcgccg ggttgtgggg cctcgcggct gcggctcgaa gccttcaggt
    13261 ctttgtagag ctgaagctcg cgctcggcgt ccgccgggcg cgcggcgcgg cggtaggcga
    13321 tgctgagctg gtagcggatg tagtccttcg caggctctgc gcgcgcggcg gcttcgaggt
    13381 gctcgatggc gccggccacg tcgccctgct ccgacagagc cttgccgaga cgatagcgcg
    13441 cgtcggcgta atcgggccgc gcggcgatgg cctctttcag cagcgtgacg gcctcggcgg
    13501 tcttctgctt cgactctagc aggacgtagg cgaggtggta tttcgacacc tcgtccgcgg
    13561 ggttcagcgc cagctcctga cgaaactcct gctcggcctc gtcgagacgg ttgaggtgaa
    13621 tcaggctgag gcccgcgttg tagtgcgcct gacgcaaggc ggggtcgagc cgcgcggcgg
    13681 cgcggtactc gcgcagcgca ccgtcgtagt cggcgatcat catgaaccct tgcgcggcgt
    13741 agagtcgcgc ctgcggcgcg cgcgcgtggc gctcggcgag gcgcgtgaag agcgcggttg
    13801 ccgccttcgc gtcggcgagg cggacgagcg agacgccgta ggcgtaggcc agctcggcgt
    13861 cgtccgtgat gacggcgtcg agcggtcgca gcgtttcgac ggcctgcttg aaattgccgg
    13921 tcaggtaata gctgacgccg agcatgcggc gcgcgagcgt gtccgccggc tgcttgagca
    13981 gatgacgcga gagcggctgg acggcctgct cgtgctggcc ggcgctgaag gagacgatgc
    14041 cccagttgcg gtcgagtccg gcgaggtcgg gcttccacgc ggcggcggcg gcgaactgcg
    14101 cggcgccctc ggcgggccgg ccctgctgcg cggcgatgac gccgaggttg tggtaagcct
    14161 gcgcgaggac ttcgccgagc cggagcttga ggctggcgta gcgcgccgcg tcgcgcgaat
    14221 cgggcgcgga ctcttcgatg agcaagacct cttgttcgtc gcgcgactcg accggggact
    14281 cttcgacgac cgagacctct gcctcgtccg cctcgtccga cttcttctcg cccgcggctg
    14341 ccgcctgact cggggccgac acgcctccgg cggacttcgc cacactgccg aggatttctt
    14401 tgatccgctg ccgcgagctt tcgagcgact gcccctgaag ctcgcgcgcc agggccagtt
    14461 ccttctcgcc ctcggccttg cgccccgcct tcatcagcaa gcgaccgagc aggaagtgcg
    14521 cacgcttgat ctggtaactg ttctgcgaaa cgtcggcggt gaggacgatg gcgcggcgca
    14581 gactctgctc ggcggcgggg tctccgagtt cggcctgcga ctggccgagg tgcaggtagg
    14641 attcgcccga ctcgggtttg agccgcacgg cctccgcgag gtgtttgacg gccttgcggt
    14701 ggtcgctctc gcccgcggcg atgaggccga ggaagaagtg cgcgtagaag tcccgcgggt
    14761 tcttttgcag ttgacgttcg aactccgccg ccgcctcggc gacgcgctcg cccccgccgt
    14821 tcatgaggat gacgtagccg aggtagaagt gggcgcgcgg cgcggcgggg tcgatggcga
    14881 gcgcgcgttt gaactcgcgc tcggcctccg cggggaagcc cgtctcctcg taggcccgac
    14941 cgaagaggat gtgcgcgacg gcgctgtcgc cgagggccgt ctgcatctct tcgaagagca
    15001 gcttggcgcg gtcgagttct ttcaggcgca ggtacgtcat gccgagcgtg taggccgcgt
    15061 cgaggtcggt gccgagcacg accgcgcgct caagctcgcg ccgcccgccc gcgtagtcgc
    15121 ccttcatgta gaggagcttg ccgaggacgt ggcgggcgcg cgcgtcgcgc tcgtcggacg
    15181 cggcggcggc ctgcgcttcg gcgacggccc ggtcgatctc catcaggcgc atgtgcgcga
    15241 tggctaggtc ggttcggacg cgcgcgtctt cgcgcagcga gagcgaatcg gacaggaggc
    15301 gcgcggcgcg ctgaagctgg cgctcgcgga tggcgagggc gccgaggtgg cccagggcga
    15361 cggcgacgac ggcgcggttc tcggcggcgg cgcgcgcgat gtcgccggag agctggtagg
    15421 tctcagccgc gccgaggtgg cgttcgagct ggtctggttg gggtctttgc gccgggttgg
    15481 gtttgccggc gggctgttgt ggctgtggct gctgctgtgg gcgctgctgc ggctgcggct
    15541 gctgctgttg ggccgccccg gcggcgggca gccacagcga gagggaaagc aatccgaggg
    15601 acagaaactt gctcacggtc ttcagggtct ttcgggcgaa ttcgaacggc aggctctcgc
    15661 gtgaataata acagggaggc aaagccgaac gtcaagcttt gcctccctgt cttgcccaga
    15721 gccggggtcg aagtttagaa gaccagtttc agcgcgtact ggatttcgcg cgggccgcgc
    15781 gtcgtgcccg accgaccgaa gttgccgttc accggaaccg ggatgggcac tgtcggatta
    15841 ggagcatcag ccggtttaag tccctgggtg atgacccggt tggttgccga gcaattgaac
    15901 gtggtcggca tcccggcgca gttgacgttc cccccggcca aattcatgtt gttctgcaaa
    15961 ctgtcgccgc ggaactgcgt gtggttgaag gcgttgaaca gctccagccg gaactgcacg
    16021 cgcgcccctt cgccgaacgg cttcctgatc cagccggggg tga
//
LOCUS       DBNGOH010000007         3332 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1650575, whole genome shotgun sequence.
ACCESSION   DBNGOH010000007 DBNGOH010000000
VERSION     DBNGOH010000007.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3332)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3332)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3332
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1650575"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..241
                     /locus_tag="AC159P_00125"
     CDS             <1..241
                     /locus_tag="AC159P_00125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3693884.1"
                     /translation="ERSRKTRRPARVRNASARSTDDLPDANLSLTNYELVLPGRDKIL
                     GTEDDLRIRDGLIIEASKEAGGAAAARPLASAKKN"
     gene            280..570
                     /locus_tag="AC159P_00130"
     CDS             280..570
                     /locus_tag="AC159P_00130"
                     /inference="COORDINATES: protein motif:HMM:NF023580.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HIR3693885.1"
                     /translation="MGIFDLFRRKQVDGEATRRAALLRTGRITEGSIFDVITDADGAI
                     TQVFYNYEISGVEYESSQSLDAGQRERSSDYYPGARVTVRFNPRRPGNSVVV"
     gene            739..1845
                     /locus_tag="AC159P_00135"
     CDS             739..1845
                     /locus_tag="AC159P_00135"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004387324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HIR3693886.1"
                     /translation="MRIATFNVENLFSRARALNQDTWAEGKPILAAYSRLNTRLQKPI
                     YSNADKQAILADLEQLGLKRSDESTFALLRQNRGKLVKRPSNAAPQIVAAGRGDWIGW
                     VELKTEAVNEVATRMTAKVIQDVNADVLAVVEAEDRVALKRFNDQLLKTINATYDCIM
                     LIDGNDERGIDVGLMTKSGFDIGGIISHVDDSVGGVRTFSRDCPEFTVRAGEARLLVM
                     VNHFKSKGFGTPAESNARRKAQAARVREIYEQRRQEGFEAIVIVGDLNDTPGSDPLKP
                     LLGDGSDLRDITEHPSFESDGRPGTYANGTASNKIDYLLLSPALWDKVTGGKIWRMGV
                     WGGVNGTIFPHYDEMEKAAHAASDHAAVWADIDL"
     gene            1909..>3332
                     /locus_tag="AC159P_00140"
     CDS             1909..>3332
                     /locus_tag="AC159P_00140"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HIR3693887.1"
                     /translation="MFRKILIANRGEIAVRVMRACRELNISPVAVYSEADAAALHVRL
                     ADEAYAVGPAPSAESYLRIDKIIEVALRSGAEAIHPGYGFLAENAAFARAVGEAGLAF
                     IGPTPEAMELMGSKTSARRAAIAAGAPVVPGTTEPLGSLDEAREVSARLGYPLMLKAA
                     AGGGGKGMRLVASEGELASAFETARSEAASAFGDSSVYIEKAVERPRHIEIQVFSDTR
                     GNHVHLGERECSIQRRHQKVIEECPSPIDDADLRERMGAAAVGIARACGYVGAGTVEF
                     LVSDVTREFYFLEMNTRLQVEHPVTELVTGIDLVREQIAVAAGHPLSFTQEDVRWTWH
                     AIECRVYAEDTDNNFMPSPGRITSLRVPAGPCVRDDGGVYEGAEVSIYYDPMISKLAT
                     WGRTRVEAIERMRRALGEYTVGGIKTTLPFFREVMRDEEFVAGRLDTGFIARFNERRA
                     HSHDEPRDGEGDEARAAAGEEEAVT"
BASE COUNT          595 a         1114 c         1120 g          503 t
ORIGIN      
        1 cgagcggagc cgcaagacgc gccgcccggc gcgcgtccgc aacgcctcgg ctcgcagcac
       61 ggacgatctg ccggacgcga acctgtcgct caccaactac gagctggtgc tccccggcag
      121 ggacaaaatc ctcggcaccg aggacgacct cagaatccgc gacggcctca tcatcgaagc
      181 atcgaaggaa gcgggcggcg ccgccgccgc gcggcccctc gcgtcggcga agaagaactg
      241 accgacaacc gccctcgcgt taacttcccc ctccgagcta tgggcatctt cgatctcttc
      301 cggcggaaac aggtggacgg cgaggccacg cgccgcgccg cgctgctccg cacgggtcgc
      361 atcacggaag gctccatctt cgacgtgatc accgacgccg acggcgccat cacgcaggtc
      421 ttctacaact acgagatcag cggcgtcgag tacgagtcct cgcagtcgct cgacgccggc
      481 cagcgcgagc gctcctcgga ctactacccc ggcgcgcgcg tcaccgtccg cttcaacccg
      541 cgccggcccg gcaactccgt ggtggtttga tatgaagtga cggttgacgg tcggcaggcc
      601 ggaacgggtt ttcgacttgt tgcccgccgc ctctctccac gcaacggact tccagcgtct
      661 cttacccggc accgcgtcgt aagactagac ccgcgccgcg agtgagccga tcttcaccat
      721 ccgaaaggag ttcacgccat gcgcatcgcg accttcaacg tcgaaaacct attctcccgc
      781 gcgcgcgcgc tcaaccaaga cacttgggcc gaaggcaagc cgattctggc cgcttacagt
      841 cgcctgaaca cgcgccttca gaaacccatc tacagcaacg cggacaagca ggcgatcctc
      901 gcagacctcg aacagctcgg cctcaaacgc agcgacgagt ccacgttcgc gctcctgcgg
      961 cagaacaggg gcaagctcgt caaacgcccg tcgaacgcag cgccgcagat cgtggccgcg
     1021 ggccgcggcg actggatcgg ctgggtcgag ttgaagacgg aggcggtgaa cgaggtcgcg
     1081 acgcgcatga ccgcgaaggt gattcaggat gtcaacgcgg acgtgctcgc cgtcgtcgag
     1141 gcagaggatc gggtcgccct caagcgcttc aacgaccaac tgctcaagac gatcaacgcg
     1201 acttacgact gcatcatgct gattgacggc aacgacgagc gcggcatcga tgtcggtctc
     1261 atgaccaagt ccggcttcga catcgggggg ataatcagcc acgttgacga ctccgtgggc
     1321 ggcgtcagaa ccttcagccg cgactgcccg gagtttacgg tgcgggccgg agaggcgcgt
     1381 cttctggtca tggtgaatca cttcaagagc aagggcttcg gcacgcccgc ggagtcgaac
     1441 gctcgtcgga aagcacaggc cgcgcgggtg cgcgagattt acgagcagcg caggcaggag
     1501 gggttcgaag ccatcgtgat cgtcggcgat ctcaacgaca cgcccggcag tgatccgttg
     1561 aagccgctcc tcggcgacgg gtccgacctg cgcgacatca ccgagcaccc gtccttcgag
     1621 agcgatgggc gccccggcac gtacgccaac ggcacggcgt cgaacaagat cgactacctg
     1681 ttgctttcgc ccgccctttg ggacaaggtc acgggcggga aaatctggcg catgggcgtg
     1741 tggggcggcg tcaacgggac gatattcccg cactatgatg agatggagaa ggcagctcac
     1801 gccgcgtccg accacgccgc cgtttgggcc gacatcgatc tctgacggct accggactct
     1861 gacaacgcgg cctcgctggc ccacgccggc gaggccgcca atgattccat gtttcgcaaa
     1921 attctcatcg ccaaccgcgg ggagatcgcc gtgcgcgtga tgcgcgcgtg cagggagttg
     1981 aacatctcgc cggtggccgt ctattcggag gcggacgcgg cggcgctgca cgtgcgtctg
     2041 gcggacgagg cgtacgcggt cggccccgcg ccgtcggctg aaagctatct gcggatcgat
     2101 aaaatcatcg aggtcgcgtt gcggagcggc gcggaagcga tacaccccgg ctacgggttt
     2161 ctggcggaga acgccgcgtt cgcgcgcgcg gtcggggaag cgggcctcgc gttcatcggg
     2221 ccgacgcccg aggcgatgga gctgatgggt tcgaagacga gcgcgcggcg cgcggccatc
     2281 gcggcgggcg cgcccgtcgt gccggggacg accgagccgc tcgggtctct ggacgaggcg
     2341 cgcgaggtgt cggcgcgttt gggctatccg ttgatgctca aggcggcggc gggcggcggc
     2401 ggcaagggca tgcgcctcgt cgcgtcggaa ggcgagctgg cgtcggcctt cgagacggcg
     2461 cggtcggagg cggcgtcggc cttcggcgat tcgtccgtct acatcgagaa ggccgtcgag
     2521 cgcccgcgcc acatagagat tcaggtcttc tccgacacgc gcggcaacca cgtccacctc
     2581 ggcgagcgcg agtgctcgat ccagcgccgc caccagaagg tgatcgagga gtgcccttcg
     2641 ccgattgacg acgccgattt gagggagagg atgggcgcgg cggcggtcgg gatcgcgcgc
     2701 gcgtgcggct acgtcggcgc gggcaccgtc gagtttctgg tctcggacgt gacgcgcgag
     2761 ttctacttcc ttgagatgaa cacgcgcttg caggtcgaac acccggtgac ggaactcgtc
     2821 acgggcatcg acctcgtgcg cgagcagatc gcggtcgccg ccggacaccc gctctcgttc
     2881 acgcaggagg acgtgcgctg gacgtggcac gccatcgagt gccgcgtcta cgccgaagac
     2941 acggacaaca acttcatgcc gtcgcccggt cgcatcactt cgctgcgcgt gcccgcggga
     3001 ccgtgcgtgc gcgacgacgg cggcgtctac gagggcgcgg aagtctccat ctactacgac
     3061 ccgatgatct cgaagctcgc cacctggggg cgcacgcgcg ttgaggccat cgagcgcatg
     3121 cgccgcgcgc tcggcgagta cacggtcggc ggcatcaaga ccacgctccc cttcttccgc
     3181 gaagtgatgc gcgacgagga gttcgtcgcc ggtcgcctcg acacgggctt catcgctcgc
     3241 ttcaacgagc gccgcgcaca ctcgcacgac gaaccgcgtg acggcgaagg cgacgaagcg
     3301 cgcgccgcgg cgggcgaaga ggaggcggtc ac
//
LOCUS       DBNGOH010000008         5270 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4033440, whole genome shotgun sequence.
ACCESSION   DBNGOH010000008 DBNGOH010000000
VERSION     DBNGOH010000008.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5270)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5270)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5270
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4033440"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1348
                     /locus_tag="AC159P_00145"
     CDS             <1..1348
                     /locus_tag="AC159P_00145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3693888.1"
                     /translation="YLEGQLARLAREGKNGTAIHILDDPLAASPQANGAPGGDEIFVW
                     IRNDLFAASPQIENLRRLATTLDNASANRFVGTSFHTRLAEIYREGAGLVVAADLEKL
                     VGQVAREDGNTPGGAERVDGLRRLGLTNLKHLVVEQKDSNERTQSRAVLTFSEPRRGI
                     ASWLAAPGAMGALEFVSPDANVATAFVVKEPALLVDDLLGFLETVQPDLRRQLREIEA
                     QQGIDIRRDLAAPLGGEFAFAIDGPLLPTPSWKMILEVNDQAHLQGSLERVVEKLNGW
                     AKLSGKGGLRWENTTEGGRTTYTLRSADFGLEVHYTFVNGYLVVTPSRALLDRALKFR
                     ESGNTLLRSTRFRETMPSDGNANFSAVLYHDLAPALRPLAERAPESQRGKLAAAAEMP
                     PTLAYAYAQGDRITLAANTEGGPFGLGPASLLGLPNAFAMQNILMNAMGEKDESKR"
     gene            1510..2472
                     /locus_tag="AC159P_00150"
     CDS             1510..2472
                     /locus_tag="AC159P_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3693889.1"
                     /translation="MNPIIELDGLSVTFGRRAILKDLSGSVKGRAVGLLGPNGAGKTT
                     LIHTLLGFHAPSSGTVRILGEDIATNARSLRAAVGYMPERDSFIARMSCVRFVRLMAE
                     LSGIPSEAALERAHEALFYVGLGEARYRSVDTYSLGMKQLAKLAQAIVHGPRLLILDE
                     PTNGLDPPTRARMIRLIQEIGDSGRARILLSSHLLRDVEECCDEILVLKDGRIAANCN
                     LDEERKANRKFIELETRGGDRAAFAEAIGGLGCEYALAGERRIKLVMQDGVEIDDLYR
                     LAFERQIQIRRLNFKRDSLEDIFLKAMEGEENGVAEGGNGDGRL"
     gene            2459..3376
                     /locus_tag="AC159P_00155"
     CDS             2459..3376
                     /locus_tag="AC159P_00155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3693890.1"
                     /translation="MAVFENSYRQYEGRLTPGWARFLVLPRYALRDVFGSKLLTAIFC
                     LCFIYPLVAAILIYLHHNTNAVALLQIDVRELLPINASFFQYFVEFQGWLAFILTVLV
                     GPVLVSRDLANNALPLYLCRPFSRAEYVLGKMTVLVILLSLVTWVPGLLLFLLQAYLE
                     GAGWIVSNAWMAGAIFVGSMTWIVLLSLLALAISAWVRWRVVASGALLGLFFITSALG
                     NLVYGLFLTRMGHLISPADLMKYIWRGLFGNFTREMGRFNMEDAWGRTVDVVLREPPL
                     WACWLVVALVCLACLWLLARKVRAYEVIR"
     gene            3373..4338
                     /locus_tag="AC159P_00160"
     CDS             3373..4338
                     /locus_tag="AC159P_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3693891.1"
                     /translation="MTTAAATSERMIVFEGVSKFYGEILGVNRVNLSIAPGITSLVGP
                     NGSGKTTLMNLMTGLLRPTRGTISVLGTSPDDPEKLFRKVGYCSQFDSFPRGMTGREF
                     IEFYLSVHGYARGEVRDLTQAALERVSMLDAAERKVAGYSKGMRQRIRLAQSIAHNPS
                     VLILDEPLNGLDPMARAEIIRLFQSLAAEGLYLVISSHILHEVDMMSDSVVLMHNGYV
                     VAEGDVRGVRDEIDEHPMQILVRCDRPQVLAVRLFEHEHTIEARIHDDRRGLFVKTRR
                     PDDFYLLLNRLVTENNLQVESVAPADDDLNAVYQYLISSDGGRVS"
     gene            4335..5210
                     /locus_tag="AC159P_00165"
     CDS             4335..5210
                     /locus_tag="AC159P_00165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3693892.1"
                     /translation="MSSTVETREKRVAGGGEVANLDWSLRARQTLAVLRLELVKNFLG
                     RRALLLYVLAGLPPAVLAAVAVFLPPGASGTFAESSTAYSVIYGGLILRTLLFFGCAW
                     VFMNLFRGEVVDRSLHYYFLAPVRREVLVVGKYLSGLVATVTLFGISTLGSLALLYFG
                     RFPSQSMEYFFDGPGFGQALTYTGITMLACLGYGAVFLIVGLFFRNPIIPAALIYGWE
                     WINFLLPPLLKKVSVIHYLHSLAPVPPPSGPFVVLAEPTPAWISVPSLLIFTALTLVL
                     AGLHIRRQEISYAGE"
BASE COUNT          876 a         1703 c         1686 g         1005 t
ORIGIN      
        1 gtacctcgaa gggcagcttg cacggctcgc ccgcgaaggc aagaacggca ctgcgattca
       61 catcctcgat gacccgctcg cggcgtcccc acaagcgaac ggcgctcccg gtggagatga
      121 aatcttcgtc tggattcgca acgatctttt cgccgcgtcg ccgcagatcg agaacctgcg
      181 tcggctcgcg acgacgctcg acaatgcgag cgcgaaccgc ttcgtcggaa cgtctttcca
      241 cacgcgtctt gccgaaatct accgcgaggg cgcggggctt gtggttgccg ccgacctgga
      301 aaaactcgtc gggcaggtcg cgcgcgagga cggcaatacg ccgggcggcg cggagcgtgt
      361 cgatggcctc cgccggctcg gactgacgaa cctgaaacac ctcgtcgtcg agcagaagga
      421 ttcgaacgag aggacgcaga gccgcgccgt gctcaccttc agcgagccgc gccgcgggat
      481 cgcttcgtgg ctcgccgcgc cgggggcgat gggcgcgctt gagttcgtct cgccggacgc
      541 gaacgtggcg accgccttcg tcgtcaaaga acccgcgttg cttgttgacg acctgctcgg
      601 cttccttgaa accgtacagc ccgatctgcg ccgccaactg cgcgagatcg aggcgcagca
      661 aggcatcgac atccggcgcg acctggccgc gcccctgggc ggcgagttcg ccttcgccat
      721 cgacggcccg ctgctcccga ccccctcctg gaagatgatc cttgaggtga acgatcaagc
      781 gcacttgcag gggtcgcttg agcgcgtggt cgagaagctg aacggctggg cgaagctgag
      841 cggcaagggc ggattgcgct gggagaacac gaccgagggc ggacgcacga cgtacaccct
      901 gcgttcagcc gacttcggac tcgaagtgca ctacaccttc gtgaacggct acctcgtcgt
      961 cacgccgagc cgcgcgctgc tggatcgcgc cttgaagttc cgcgaatccg gcaacaccct
     1021 cttgcgttcg acgcgcttca gagagacgat gccgtcggac gggaacgcta acttctccgc
     1081 ggttctctac cacgacctcg cgcccgccct tcgccccttg gcggaacgcg cgcctgagag
     1141 tcagcgaggg aagctcgccg ccgccgccga gatgccgccg accctggcct acgcttacgc
     1201 gcagggcgac cgcatcacgc tcgcggcgaa caccgaaggc gggccgttcg ggttaggtcc
     1261 cgccagcctg ctcggcctgc cgaacgcgtt cgcgatgcag aacattctga tgaacgcgat
     1321 gggcgagaag gacgaatcga agcggtaagg tcggggaact cggagtgacg atcaagggga
     1381 gtaaaggcag gggactcgcc ccctgccttc tttgacggcg agccgcgcgg gcagagggcg
     1441 agtcctctgc ccgcgcgatt ttattcagaa catcgtcacg cctgagaccg tagaaagtct
     1501 ctccggctta tgaaccctat catcgaactc gacggtttga gtgtcacgtt cggcaggcga
     1561 gcgatcctga aggatttgag cgggtcggtc aagggacgcg ccgtcgggct gctcggcccg
     1621 aacggcgcgg gcaagacgac gctcatccac acgctgctcg gctttcacgc gcccagctcc
     1681 ggcacggtgc gcatcctcgg ggaagacatc gcgacgaacg ccaggagcct gcgcgccgcg
     1741 gtcggctaca tgccggagcg cgattcgttc atcgccagaa tgtcttgcgt ccgcttcgtc
     1801 cggctgatgg ccgaactctc cggcatccct tccgaagcgg cgctcgaacg cgcgcacgaa
     1861 gccctcttct acgtgggcct cggcgaggcg cgctaccgct cggttgacac ctattcgctc
     1921 ggcatgaagc aactcgccaa actcgcgcaa gccatcgtgc acgggccgcg tctgctcata
     1981 ctggacgagc cgacgaacgg actcgacccg ccgacgcggg cgcgcatgat ccgccttatt
     2041 caggagatag gcgacagcgg cagggcgcgc atcctgctct cgtcacacct gctgcgcgat
     2101 gtcgaggaat gctgcgacga aattctcgtc ctgaaggacg ggcgcatcgc cgccaactgc
     2161 aacctcgacg aagagcgcaa agcgaaccgc aagttcatcg aactcgagac gcgcgggggc
     2221 gaccgtgcgg cgttcgccga agcgatcggc gggttaggct gcgagtacgc gctcgcgggc
     2281 gagcggcgca tcaaactcgt gatgcaggac ggcgtggaga tcgacgacct ttaccgtctg
     2341 gccttcgagc ggcagattca gattcgtcgc ttgaacttca agcgcgattc gctcgaagac
     2401 attttcctca aggcgatgga aggcgaagag aacggcgttg ccgaaggggg gaacggcgat
     2461 ggccgtcttt gagaacagct accggcagta cgaaggcagg ctcacgccgg ggtgggcgcg
     2521 cttcctcgtc ctcccgcgct acgcgctgcg cgacgtgttc ggctcgaagc tcttgaccgc
     2581 gatcttctgc ctgtgcttca tctacccgct cgtcgcggcg atcctgatct acctgcatca
     2641 caacacgaac gccgtcgcgc tcctgcaaat cgacgtgcgc gagttgttgc cgatcaacgc
     2701 gtcgttcttc cagtacttcg tcgaatttca gggctggctc gccttcatcc tcacggtgct
     2761 cgtcggcccc gtgctcgtct cgcgggattt ggcgaacaac gccttgccgc tctacctgtg
     2821 ccgcccgttc tcgcgcgccg aatacgtgct cggcaagatg acggtgctcg ttattctgct
     2881 gtcgctcgtg acgtgggtgc cgggactcct gctcttcctc ctgcaagcgt atctggaagg
     2941 cgcgggctgg atcgtgtcga atgcgtggat ggcgggggcg atcttcgtcg ggagcatgac
     3001 gtggatcgtg ctgctctcgc tgctcgcgct ggcgatctcg gcgtgggtgc ggtggcgcgt
     3061 cgtggcgagc ggcgcgctgc tcggattgtt cttcatcacg tccgccctcg gcaatctcgt
     3121 ctacggcctg tttttaacgc ggatggggca tttgatcagc cccgcggatt tgatgaagta
     3181 catctggcgc ggactcttcg gcaacttcac gcgggagatg ggtcgcttta acatggagga
     3241 cgcctggggc agaaccgtcg atgtcgtgtt gcgcgagccg ccgctgtggg cctgctggct
     3301 ggtggtcgct ctggtctgcc tcgcctgcct ctggctgctc gcgcgcaaag tccgcgctta
     3361 cgaggtgatc cggtgacaac ggcggcggca acatcggagc ggatgatcgt cttcgagggc
     3421 gtctcgaagt tctacggcga gatactcggc gtcaaccgcg tcaacctgtc catcgcgccg
     3481 ggcatcacga gtcttgtcgg cccgaacggt tcgggcaaga cgaccctgat gaacttgatg
     3541 acggggctgc tgcgcccgac gcgcgggacg atcagcgtgc tcggcacaag cccggacgac
     3601 ccggaaaaac tcttccgcaa agtcggctac tgctcgcagt tcgactcctt cccgcgcggc
     3661 atgacggggc gcgaattcat cgagttctac ctgagcgttc acggctacgc gagaggcgag
     3721 gtgcgggatt tgacgcaggc ggcgctcgaa cgcgtgtcga tgctggacgc ggcagagcgc
     3781 aaggtggcgg gctacagcaa aggcatgcgg cagcgcatcc ggctggcgca gtcgattgcc
     3841 cataaccctt ccgtgctcat cctcgacgag ccgttgaacg gactcgatcc gatggcgcgc
     3901 gccgaaatca tccgtctgtt tcagtcgctc gcggcggaag gcttgtacct cgtcatttcg
     3961 agtcacatcc tgcacgaagt tgacatgatg tccgacagcg tcgtgctgat gcacaacggc
     4021 tatgtcgtcg cggagggcga cgtgcgcggc gtgcgcgacg agatcgacga gcacccgatg
     4081 cagatcctcg tgcgttgcga ccgcccgcaa gttttagccg tgcgcctctt cgagcacgag
     4141 cacaccatcg aggctcgcat tcacgacgac cggcgggggc tgttcgtcaa gacgcgccgg
     4201 cccgatgact tctacctcct gctcaaccgg ctcgtgacgg agaataatct tcaggtcgaa
     4261 tcggtcgccc cggcggacga cgacctgaat gccgtttacc aatacctgat cagttccgac
     4321 ggagggcgcg taagttgagt tccacggttg agacgaggga gaagcgtgtg gcggggggcg
     4381 gcgaggtggc gaatcttgac tggtcgctcc gggcgcggca gacgctcgcc gtcctgcgcc
     4441 tcgaactcgt aaagaacttc ctcgggcgcc gtgccctgct gctctacgtg ctcgcggggc
     4501 tgccgccggc ggtgctcgcg gcggtcgccg tcttcttgcc gcccggcgcg tccgggacgt
     4561 tcgcggagtc gagcaccgct tactccgtca tctacggcgg gctgatcctg cgcacgctcc
     4621 tcttcttcgg ctgcgcgtgg gtcttcatga atctgttccg cggcgaggtc gttgaccgga
     4681 gtttgcacta ctactttctc gcgcccgtgc gacgcgaagt gctcgtcgtc gggaaatact
     4741 tgtcggggct ggtggcgacc gtcacgctgt tcggcatctc gacgctcggc tcgctcgccc
     4801 tcctctactt cgggcgcttt ccgtcacaga gcatggagta ttttttcgac ggccccggct
     4861 tcgggcaggc gctcacctac accggcatta caatgctcgc gtgcctcggt tacggggcgg
     4921 tttttctgat cgtaggtttg ttcttcagaa acccgatcat cccggcggcg ctgatctacg
     4981 gctgggagtg gatcaacttt ctgctgccgc cgttgctgaa gaaggtgagc gtcattcatt
     5041 accttcactc gctcgcgccg gtccccccgc cgtcaggccc cttcgtcgtg ctcgccgagc
     5101 cgacgcccgc ttggatttcc gtccccagcc tgctcatctt caccgcgctg acactcgtcc
     5161 tcgccggcct tcacatccgc cgacaagaga tcagctacgc gggtgaatag acgtgattct
     5221 tttgccgcgc gagacattag tccctcctta cacgatggtg gtatgccccc
//
LOCUS       DBNGOH010000009         9744 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3117000, whole genome shotgun sequence.
ACCESSION   DBNGOH010000009 DBNGOH010000000
VERSION     DBNGOH010000009.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 9744)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9744)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9744
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3117000"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..485)
                     /locus_tag="AC159P_00170"
     CDS             complement(<1..485)
                     /locus_tag="AC159P_00170"
                     /inference="COORDINATES: protein motif:HMM:NF012451.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HIR3693893.1"
                     /translation="MAKEDYYQVLGIKRDAKPEEIKKAYRRLARKFHPDVNPGDKSAE
                     ERFKKITEAHDVLSDPKKRTVYDRFGQYSDNLADMAAKGAAPGGGAGGAGRSAPFDFT
                     GFDWGNTTTSAGASGGGGSSFRDIFADLFGGGGGKQEKEPPRPMPKRGDDIEMPLALS
                     F"
     gene            complement(577..2517)
                     /gene="dnaK"
                     /locus_tag="AC159P_00175"
     CDS             complement(577..2517)
                     /gene="dnaK"
                     /locus_tag="AC159P_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HIR3693894.1"
                     /translation="MSKIIGIDLGTTNSVVAVMEGGEPTVITNSEGGRTTPSVVAFAK
                     DGNRLVGQVAKRQAVTNPENTVYSIKRFMGRRYNEVSEEMKQVPYKVKSAGNDVRFEA
                     GGKEWSPPEISAMILQKLKQAAEDYLGQKVDKAVITVPAYFNDSQRQATKDAGRIAGL
                     EVMRIVNEPTAAALAYGLDKKKDETIAVFDFGGGTFDISILEVGEGVVEVKSTNGDTH
                     LGGDDIDERLIEWIIEEFKKDQGIDLSNDKMALQRLKEAAEKAKIELSSTMETEINLP
                     FITADQSGPKHLVMKLTRARFEQLIEQILQRLKHPVEQAIKDAGLDANKIDEVVLVGG
                     STRIPKVQEMVKGIFGKEPNKSVNPDEVVAVGAAVQAGVLSGEKTDILLLDVTPLSLG
                     IETLGGVFTKLIERNTTIPTRKSEIFSTASDNQTSVEVHVLQGERQMASDNRTLGKFH
                     LVGIPPAPRGIPQVEVTFDIDANGIVNVSAKDMGTGREQKITITSSSGLSKDEIDKMM
                     REAEAHAGEDAKKKEEIEARNRLDGAVYQWEKLYTENKEKLDGATSTQIEAAIADAKK
                     ALEEGGSERMNDAFNNLQTAMNKMSEALYNSGAASGGATGGDEQANAAGASSGSAGGG
                     ATSGGGDDNVIDAEYVDVDENK"
     gene            complement(2778..3389)
                     /locus_tag="AC159P_00180"
     CDS             complement(2778..3389)
                     /locus_tag="AC159P_00180"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HIR3693895.1"
                     /translation="MSSRIATVPNLLTVVRLVLVPVFVSALYYQRFGWALFVFVVAGV
                     TDSLDGLLARRFNQGSQLGQILDPIADKLLLVTSFVTLSMHSIMEPSALVQPHPRHLP
                     VPFWVTAAVISRDIFIVVGAAAINIVTGFRGFRPSWLGKANTVVQITAVVLILVAASF
                     PPLRGYLPTVYTTVFAFAVISGAHYVFWIAKLLNEDRATRETR"
     gene            complement(3478..4263)
                     /locus_tag="AC159P_00185"
     CDS             complement(3478..4263)
                     /locus_tag="AC159P_00185"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024258.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HIR3693896.1"
                     /translation="MAAGLVQLARAAFAEPYQLAVEHTAIGLKRLPRELDGFRIVHLS
                     DIHHSPFTGRQQVERAVEVANSLQPDIIALTGDYVSHEREYVQPCADMLGRLRARRGV
                     YAVLGNHDNWVDAELVTDLFTLAGIRVLNNEGMRFEDRGASFWLAGVQDTMVGLEDLP
                     LALAGSADAEMKLLLAHNPVILRRAARAGVDLVLSGHTHGGQVTWRSERSASGRVRRR
                     ILRGLGRRGATQIYVTRGLGTVVLPVRYGCLPEVSVLTLRCTD"
     gene            complement(4552..6411)
                     /locus_tag="AC159P_00190"
     CDS             complement(4552..6411)
                     /locus_tag="AC159P_00190"
                     /inference="COORDINATES: protein motif:HMM:NF015557.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HIR3693897.1"
                     /translation="MMMTPAIATVLVLLLVALVLFATERIPIDIVTLMLVVGLVLTGT
                     LTAAEAFAGFGNDIVITIAGLFVLTGGLVKTGVVDVIGRRLHHISGGDEFRLTALIMF
                     TAAACASMMKNTTTTAMFVPVVLGLAERAHVRPSKLLMPLAFGAILGGTCTLIGTSTN
                     LAVSGALSRLGDSPELAARLADSPELLARFGPPQALFEPFSMFELTPVGIAVVVAGMA
                     YMLLLGLRLLPARGGGDVSLVEQYQIREYVSEVIVLPDSPLVGQTLAEANLSETLDLT
                     VIGIIRGVQARTAPRAEETIQSDDLLLVQGRAEDILRVKREAGIEIKPDFELSPRDLE
                     AGDIELFEVMVLRGSDLNGRTLRGLNFRDRYDLTVLAINRHGVALLSKLSSVTFRFGD
                     VLLVQGSRESVERLAREGNLLLLEEAAERHERAGKRVWAVAAFLLFILLSITKLVPLS
                     VAILLGVLLLLATRTVRAQEIYGLVDWRLIVLIAGMISFGTAMERSHADQFLADLIVR
                     YFEAYGGTAVLAGFFALTVALTQPMSNQAAALVVLPIAVKTALLLGLNPRTFAVTVTY
                     AASCSFLTPLEPACVLIYTPGRYRFFDFVRVGSVLTLAVFAIVILLVPIFWPL"
     gene            6539..7045
                     /locus_tag="AC159P_00195"
     CDS             6539..7045
                     /locus_tag="AC159P_00195"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3693898.1"
                     /translation="MAAPQRILVIEDQEDLAALYESALEREGYRVTKAYTGEEGVALF
                     QDDGADAVLLDMTLPEMNGLQTLRQVRALDARVPVVVATGETSDDTRRQCARLGVHEY
                     LSKPVDHTDLARSLRRALDDPKTTTEEYEVVTLRLPTRVVKVLTGIDENLENAVTRLC
                     EEKLKAGS"
     gene            complement(7095..8117)
                     /locus_tag="AC159P_00200"
     CDS             complement(7095..8117)
                     /locus_tag="AC159P_00200"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3693899.1"
                     /translation="MLRVEYRDGLYLPDIDLWLDADGEREHCVISHAHSDHIAGHSSI
                     VATPETARIFRHRIGETEVVTQRYGERRDYGRYALTFYPAGHCLGSAQILVEAEGERL
                     VYTGDIKLRPNVAAEQAVVVPCDTLVMESTFGDPQYVFPPDVATFDRLYAAVERAFSD
                     HRVPVVLAYALGKSQEALELLLRRGYRVSLHGSVWNVSEIYRELGVKFSGEYERYDRE
                     RLKGRVLIAPPGCRKQPMITNIERRYLVMLTGWAMSKSAPYMYKGVDLVLPLSDHADF
                     DELVRLAKESGATRIITMHGAKKFAAILRDMGLNAEHLAQHPGAASAKGKVKRAATAV
                     EQKLFE"
     gene            8306..9031
                     /locus_tag="AC159P_00205"
     CDS             8306..9031
                     /locus_tag="AC159P_00205"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013718.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HIR3693900.1"
                     /translation="MTDEAGNVIRTEAEAAREGRRQRFRAVRRLLRAEVYYGAALAAF
                     AGLTAFAYFNAYFGWDLRAARAVQGVGGLYDFMRLVSRFGDGWAPIALTTATALGFLL
                     FRRRSEAFGLIFSAGGSAFLARLIKTTVGRPRPAADLVNVFSEGDLLSFPSGHVTFYV
                     TYFGFLFFVAYALLPRASLARRLALGLTALPVLLIGLSRVYLGRHWPSDTLGAYLMGG
                     LWLALALDLYRRWKKKPMEETQK"
     gene            complement(9201..9716)
                     /locus_tag="AC159P_00210"
     CDS             complement(9201..9716)
                     /locus_tag="AC159P_00210"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HIR3693901.1"
                     /translation="MLMAATALISATAAAPFADPPAVGAAAPEFTLTTNEGKQASLKD
                     YRGKWVVLYFYPKDFTSGCTLQARNFQRDQAKYAELNAVVLGVSVDTAESHKEFCAKE
                     GLNFKLLSDTDAKVSNAYGSVMDYNGATYSARNTFVIDPEGKVARVFIKAKPASNSEE
                     VLAALAELQKK"
BASE COUNT         1578 a         3153 c         3316 g         1697 t
ORIGIN      
        1 tcgaacgaga gcgcgagcgg catctcgatg tcgtcgccgc gcttcggcat cggtcgcggc
       61 ggctccttct cctgcttgcc gccgccgccg ccgaacaggt cggcgaagat gtcgcggaac
      121 gaagagccgc cacccccgga cgcgcccgcc gaggtcgtcg tgttgcccca gtcgaagccc
      181 gtgaagtcga acggcgcgct gcggcccgcg ccgcctgcgc cgccgcccgg cgcggcgccc
      241 ttcgccgcca tgtccgcgag gttgtccgag tactggccga agcggtcgta gacggtgcgc
      301 ttcttcggat cggagagcac gtcgtgcgcc tccgtgatct tcttgaagcg ctcctcggcg
      361 gacttgtcgc ccggattcac gtccgggtga aacttgcgcg cgagcctccg gtaagccttc
      421 ttgatctcct ccggcttcgc atcccgcttg atgccgagca cctggtagta atcttctttt
      481 gccatcacaa aaaggggtcg ggggtcaggg gccggaggcc ggtgaaagcc aagttgtttt
      541 caccggcccc cggcccccga cacccgtccc ccgctcttac ttgttctcgt ccacgtccac
      601 gtactcggca tcgatgacgt tgtcgtcgcc gccgcccgag gtcgcgccgc cgcccgcgga
      661 gccggacgag gcgcccgcgg cgttggcctg ttcgtcgccg ccggtcgcgc cgcccgatgc
      721 cgcgccgctg ttgtagagcg cctcggacat cttgttcatg gcggtctgga ggttgttgaa
      781 ggcgtcgttc atgcgctcgg agccgccctc ttcgagagcc ttcttggcgt cggcgatggc
      841 cgcctcgatc tgcgtggagg tcgccccgtc gagcttctct ttgttctcgg tgtagagctt
      901 ctcccactgg tagaccgcgc cgtcgagacg gttgcgcgcc tcgatctcct ccttcttctt
      961 cgcgtcctcg cccgcgtgcg cctcggcctc gcgcatcatc ttgtcgatct cgtccttgga
     1021 cagaccggac gacgaggtga tcgtgatctt ctgctctctc ccggtgccca tgtccttggc
     1081 cgagacgttg acgatgccgt tggcgtcgat gtcgaacgtc acctcgacct gcgggatgcc
     1141 gcgcggcgcg ggcggaatgc cgaccagatg gaacttgccg agcgtgcgat tgtccgaggc
     1201 catctggcgc tcgccctgta ggacgtgcac ctcgaccgag gtctggttgt ccgaggcggt
     1261 cgagaagatt tcgctcttgc gcgtcgggat ggtcgtgttg cgctcgatga gcttggtgaa
     1321 gacgccgccc aacgtctcga tgcccagaga gaggggcgtc acgtcgagca gcaggatgtc
     1381 ggtcttctcg cccgagagca cgcccgcctg gacggcggcg ccgacggcga ccacctcgtc
     1441 cgggttgaca gacttgttcg gctccttgcc gaagatgccc ttgaccatct cctgcacctt
     1501 cgggatgcgc gtcgagccgc cgacgaggac gacttcgtcg atcttgttcg cgtcgaggcc
     1561 cgcgtccttg atcgcctgct cgaccgggtg cttgaggcgt tggaggattt gctcgatcaa
     1621 ctgctcgaag cgggcgcgcg tcagcttcat gacgaggtgc ttcgggccgg actggtcggc
     1681 ggtgatgaag ggcaggttga tctcggtctc catcgtggag gacagctcga tcttcgcctt
     1741 ctccgccgcc tccttcaggc gctgcaaggc catcttgtcg ttcgacaggt cgatgccctg
     1801 atccttcttg aactcctcga tgatccactc gatgaggcgc tcgtcgatgt cgtcgccgcc
     1861 gaggtgcgtg tcgccgttgg tcgatttgac ttcgacgacg ccctcgccga cttcgaggat
     1921 cgagatgtcg aacgtgccgc cgccgaagtc gaagaccgcg atggtctcgt ccttcttctt
     1981 gtcgaggccg taggccaggg cggcggcggt cggctcgttg acgatgcgca tcacttcgag
     2041 gccggcgatg cggcctgcgt ccttcgtcgc ctgccgctgc gagtcgttga agtaggccgg
     2101 caccgtgatg accgccttat cgaccttctg gccgaggtag tcttccgccg cctgcttcag
     2161 cttctggagg atcatcgcgg aaatctcggg cggcgaccac tccttgccgc ccgcctcgaa
     2221 gcgcacgtcg ttcccggcgg acttcacctt gtacggcacc tgcttcatct cttccgagac
     2281 ttcgttgtag cggcgcccca tgaagcgctt gatcgaatag acggtgtttt cggggttggt
     2341 gacggcctgc cgcttggcga cctggccgac cagacggttc ccgtccttgg cgaaggcaac
     2401 gaccgaaggg gtcgtgcgac cgccctccga gttggtgatg accgtcggtt cgccgccttc
     2461 catgaccgcc acgacggagt tggttgtgcc caggtcaatg ccgataatct tactcatcgc
     2521 ttttctgctc tcccaattta tctggcccca cgcgcccacc cctcacggcg ggaacgactt
     2581 gcggcgaccg aaactcgttg cccatcgccg ccgccgacaa ctcttttccg ttgaaaatcg
     2641 cgggatgaaa ttgtcggcga cttgaatgcg tcgtcaatat aatacctgag tgcgtcattg
     2701 tcaacttatt tagtagacac gcgcgccctc cgggttgccc cgttcaagct ctttaccggc
     2761 aacggtttaa cgcggggtca gcgggtttcg cgggtggcgc ggtcttcgtt caaaagtttc
     2821 gctatccaga agacgtagtg cgcgccggag atgaccgcga aggcgaagac ggtggtgtag
     2881 acggtcggca gatacccgcg cagagggggg aaactggcgg cgacgaggat gaggacgacc
     2941 gccgtgatct gcacgacggt gttcgccttg ccgagccagg aggggcggaa gccgcggaag
     3001 cccgtgacga tgttgatggc cgccgcgccg acgacgatga agatgtcgcg gctgatgacc
     3061 gccgccgtca cccagaaggg gacgggaaga tgccgcgggt gcggctggac gagcgccgaa
     3121 ggctccatga tcgagtgcat cgagagcgtg acgaaggagg tgacgagcag gagcttgtcg
     3181 gcgatggggt cgagaatctg gccgagctgc gaaccctgat tgaagcggcg cgcgagcagc
     3241 ccgtcgaggc tgtccgtcac gcccgccacg acgaagacga agagcgccca cccgaagcgc
     3301 tggtagtaga gcgccgagac gaagacgggc acgagcacca gccggacgac ggtcaggagg
     3361 ttggggacgg tggcgatgcg ggaagacata aaaaaaggac tgaggactga ggactgaggg
     3421 ccgagtgaaa gacgcgagct ttttactcag tcctcagtcc tcagtcctca gtccttttta
     3481 atccgtacag cggagcgtca gcaccgagac ttcgggcagg cagccgtagc ggacgggcag
     3541 gacgaccgtg ccgaggccgc gggtgacgta gatttgggtc gcgccgcggc gcccgaggcc
     3601 gcgcaggatg cggcggcgga cgcggcccga ggcggagcgc tcggagcgcc aggtgacctg
     3661 cccgccgtgc gtgtggccgg agagcacgag gtctacgcct gcgcgggcgg cgcggcgcag
     3721 gatgacgggg ttgtgcgcga gcaggagctt catctcggcg tcggcggagc ctgcgagcgc
     3781 gagcgggagg tcttcgaggc cgaccatcgt gtcctgcacg cccgcgagcc agaacgaggc
     3841 gccgcggtct tcgaagcgca tgccctcgtt gttgaggaca cggatgccgg ccagcgtgaa
     3901 gaggtctgtc acgagttcgg cgtccaccca gttgtcgtgg ttgccgagca ccgcgtagac
     3961 gccgcggcgc gcgcgcagcc gcccgagcat gtcggcgcag ggctggacgt attcgcgctc
     4021 gtgcgagacg tagtcgcccg tcagcgcgat gatgtccggc tggaggctgt tggccacctc
     4081 gacggcgcgc tcgacctgct ggcggcccgt gaaggggctg tggtggatgt ccgagaggtg
     4141 gacgatgcgg aagccgtcga gttcgcgcgg gagccgcttg aggccgatgg ccgtgtgttc
     4201 gacggcgagc tggtaaggct cggcgaacgc cgcgcgcgcg agttgcacca atccggcagc
     4261 catcgtgcgg aggggagtcg cgcgccgagt ggcgacacgt cgccgttctg tccaaccggt
     4321 gagctttgcc attgtctctc tgttttccgg ggaaggtctt taaaaacgct tgcgaggccg
     4381 taaacccttc gtccctggcc cgcgtttcaa agcccgcgaa ttatacggag gtgggagaaa
     4441 aaaggcaaaa ggtaaacgcc ccccgcagcc gcccgcaagc aaaatataac atcccgccaa
     4501 ttcgccctgt gcgcggcact ttcgcctttt accactttag ttttacccgc ttcagagcgg
     4561 ccagaagatg gggacgagca ggatgacgat ggcgaagacg gcgagcgtca agaccgagcc
     4621 gacgcggacg aagtcgaaga agcggtagcg gcccggcgtg tagatgagca cgcaggccgg
     4681 ctccagcggc gtcaggaaag agcacgaggc ggcgtaggtg acggtgacgg cgaaggtgcg
     4741 cgggttgagg cccagcagga gcgcggtctt gacggcgatg ggcaggacga cgagcgcggc
     4801 ggcctggttc gacatcggct gcgtgagcgc gaccgtcagc gcgaagaagc ccgcgagcac
     4861 cgccgtcccg ccgtaggctt cgaagtagcg gacgatgagg tcggcgagga actggtcggc
     4921 gtgcgacctc tccatcgcgg tgccgaacga gatcatcccg gcgatgagga cgatgaggcg
     4981 ccaatcgacg aggccgtaga tttcctgcgc cctcacggtg cgggtcgcca agagcagcag
     5041 cacgccgagc aggatggcga ccgagagcgg cacgagcttg gtgatcgaga ggaggatgaa
     5101 gagcaggaag gccgcgacgg cccagacgcg tttgcccgcg cgctcgtgcc gctcggcggc
     5161 ctcttcgagc agcagcaggt tcccctccct cgccagccgc tcgacgctct cgcggctccc
     5221 ctgcaccagc agcacgtcgc cgaagcggaa cgtgacggaa ctcagcttcg agagcagcgc
     5281 gacgccgtgg cggttgatgg ccaagaccgt caggtcgtag cggtcgcgga agttgaggcc
     5341 gcgcagcgtg cgcccgttca agtccgagcc gcgcaagacc atcacctcga acagctcgat
     5401 gtcgccggct tcgaggtcgc gcgggctgag ttcgaagtcg ggcttgatct cgatgcccgc
     5461 ctcccttttc acgcgcagga tgtcttcggc gcggccctgc acgagcagca ggtcgtccga
     5521 ttggatggtc tcttcggcgc gcggggcggt gcgcgcctgc acgccgcgga tgatgccgat
     5581 gaccgtgagg tctaaggtct cggagaggtt cgcctcggcc aaagtctggc cgacgagcgg
     5641 cgagtccggg agcacgatca cctcggagac gtactcgcgt atctggtatt gctcgacgag
     5701 cgagacgtcg ccgccgccgc gcgcgggcag gaggcgcagg cccaggagca gcatgtaggc
     5761 catcccggcg acgacgacgg cgatgccgac cggcgtcagc tcgaacatcg agaacggctc
     5821 gaagagcgcc tgcggcgggc cgaagcgcgc caagagttcg ggcgagtcgg cgagccgcgc
     5881 ggcgagttcg gggctgtcgc cgaggcgcga caaagcgccc gagacggcga ggttcgtcga
     5941 ggtgccgatg agcgtgcacg tgccgccgag gatggcgccg aaggcgagcg gcatcagcaa
     6001 cttcgagggc cgcacgtgcg cgcgctcggc tagcccgagg acgacgggca cgaacatcgc
     6061 cgtcgtcgtc gtgttcttca tcatcgaggc gcaggcggcg gcggtgaaca tgatgagcgc
     6121 cgtcagccgg aactcgtcgc cgcccgagat gtggtggagc cggcgcccga tcacgtccac
     6181 cacgcccgtc ttgacgaggc cccccgtgag gacgaacagc ccggcgatgg tgatgacgat
     6241 gtcgttgccg aagcccgcga acgcctcggc ggcggtgagc gtccccgtca ggacgagtcc
     6301 gacgacgagc atcaacgtca cgatgtcgat gggtatcctc tcggtcgcga acagcacgag
     6361 cgcgaccaga agcagcacga gcaccgtggc gatggcgggc gtcatcatca tgggagagtt
     6421 ctgttacaat cctcgctaaa gagacacgaa tgccgaaccg ttttttttgc cgcacgcgct
     6481 tttcggttcc gcgattaaag aactaaacgg cgaagtttgc aagggggatt attccggcat
     6541 ggccgcaccg cagcgcatcc tcgtcatcga agaccaggaa gacctcgccg cgctctacga
     6601 gagcgcgctc gaacgcgaag gctaccgcgt gaccaaagcc tacacgggcg aggagggcgt
     6661 cgccctcttc caggacgacg gggcggacgc cgtcctgctc gacatgacgc tgcccgagat
     6721 gaacggcctc cagacgctcc ggcaagtccg cgccctcgac gcgcgcgtcc ccgtcgtcgt
     6781 cgccaccggc gagaccagcg acgacacgcg ccggcagtgc gcccgcctcg gcgtccacga
     6841 atacctctcc aagcccgtcg atcacacgga tctcgcgcgc tcgctccgcc gcgccctcga
     6901 cgacccgaag accacgaccg aagagtacga agtcgtcacg ctccgcctcc cgacccgcgt
     6961 cgtcaaggtg ctgaccggca tcgacgagaa cctcgaaaac gcggtgacgc ggctgtgcga
     7021 ggagaagctg aaggcgggaa gctgaacgga tcgacgacgg acagtcgccg cctcgcaccg
     7081 ccgggccgtc gcccctattc gaacaatttc tgctcgacgg cggtcgccgc ccttttcact
     7141 ttacctttcg cgctcgcggc gccggggtgt tgggcgaggt gttcggcgtt gaggcccatg
     7201 tcgcgcagga tggcggcgaa cttcttggcg ccgtgcatgg tgatgatgcg cgtggcgccg
     7261 gactctttgg ctaagcggac gagttcgtcg aagtcggcgt ggtcggagag cggcaggacg
     7321 agatcgacgc ccttgtacat gtagggcgcg ctcttgctca tcgcccagcc ggtgagcatg
     7381 acgaggtagc ggcgctcgat gttcgtgatc atcggttgct tgcggcagcc gggcggcgcg
     7441 atgagcacgc ggcccttcag gcgctcgcgg tcgtagcgct cgtactcgcc cgagaacttc
     7501 acgcccagct cgcggtaaat ctccgacacg ttccacaccg agccgtgcag gctgacgcgg
     7561 tagccgcgcc gcaacagcag ctcaagtgcc tcctgactct tccccaacgc gtaagccagc
     7621 acgaccggca cgcggtggtc ggagaaggcg cgctcgaccg ccgcgtagag ccggtcgaag
     7681 gtcgccacgt cgggcgggaa gacgtactgc gggtcgccga aggtggactc catgaccagc
     7741 gtgtcgcagg ggacgacgac ggcctgctcg gcggcgacgt tgggccgcag cttgatgtcg
     7801 cccgtgtaga cgagccgttc gccttcggcc tcgacgagga tttgcgccga gccgaggcag
     7861 tggcccgccg ggtagaacgt cagcgcgtag cgaccgtagt cgcgccgctc gccgtaacgc
     7921 tgcgtgacga cttccgtttc gccgatgcgg tggcggaaga tgcgcgccgt ctcgggcgtg
     7981 gcgacgatgg aagagtggcc ggcgatgtgg tcggagtgcg cgtgcgagat gacgcagtgc
     8041 tcgcgctcgc cgtcggcgtc gagccacagg tctatgtccg gcaggtacag cccgtcgcgg
     8101 tattcgactc tcagcaccgt aagacacccc ctccgaggtt cggcggccag accgcgacaa
     8161 actgccggcg cgcgctcggc gccccggcgc ttgccgccgg cccgcggctt caagtatgtt
     8221 tcgggagatt cggtgcgccg attataacac gccgcgcgca gcccgcgggg ccgcctcggc
     8281 gcgagacctt ttcgagagac gacgcatgac agacgaggcc gggaacgtca tccgcacgga
     8341 ggccgaggcc gcgcgggaag ggcggcggca aaggttccgc gcggtgcggc gcctgctgcg
     8401 cgcggaggtc tactacggcg cggcgctcgc cgccttcgcg gggctgacgg ccttcgccta
     8461 cttcaacgcc tacttcggct gggacctgcg cgcggcgcgc gccgtgcagg gcgtgggcgg
     8521 cctctacgac ttcatgcggc tcgtctcgcg cttcggcgac gggtgggcgc ccatcgcgct
     8581 gaccaccgcg acggcgctcg gcttcctcct cttccgccgc cggagcgagg cgttcggcct
     8641 catcttcagc gcgggcggca gcgccttcct cgcgcggctc atcaagacga cggtcgggcg
     8701 cccgcgcccc gccgcagacc tcgtcaacgt cttctcggaa ggcgacctcc tgagcttccc
     8761 ctcgggccac gtcaccttct acgtcaccta cttcggcttc ctcttcttcg tcgcctacgc
     8821 gctgctgccc cgcgcctcgc tcgcgcgccg cctcgcgctc ggcttgacgg cgctgcccgt
     8881 cctgctcatc ggcctctcgc gcgtctacct cggtcgccac tggcccagcg acacgctcgg
     8941 cgcctacctc atgggcggcc tctggctcgc cctcgcgctc gacctctacc gccgctggaa
     9001 aaagaagccg atggaagaga cgcaaaagta gagggggaaa aggttaaaag ggggagaggc
     9061 gaaaaggaag acaagaaaca gcgtgcctct catcattcgt tgatgagagg cacgctgttt
     9121 tcagtcctca ttcgaggatg agaagcagcg tgcctctcat ccttcctttt cccctcttcc
     9181 ccttttaacc ttttcccctt ctactttttt tgcagctcgg ccagcgcggc gagcacctct
     9241 tcgctgttgc tggcgggctt ggccttgatg aagacgcggg cgaccttgcc ctcggggtcg
     9301 atgacgaagg tgttgcgcgc cgagtaggtg gccccgttgt agtccatcac cgagccgtag
     9361 gcgttcgaga ccttcgcatc cgtgtccgag agcagcttga agttcagccc ctccttggcg
     9421 cagaactcct tgtgcgactc ggcggtatcc acgctgacgc cgagcacgac cgcgttcagc
     9481 tcggcgtact tggcctggtc gcgctggaag ttgcgcgcct ggagcgtgca gcccgaggtg
     9541 aagtccttcg ggtagaagta gagcacgacc cacttgccgc ggtaatcctt gaggctcgcc
     9601 tgcttgccct cgttcgtcgt cagcgtgaac tcgggggcgg cggcgccgac ggcgggcggg
     9661 tctgcgaacg gggcggcggc ggtcgccgag atgagcgccg tcgcggccat caacacggcg
     9721 gcgcagatgc acagagcttt cttc
//
LOCUS       DBNGOH010000010         3443 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1650887, whole genome shotgun sequence.
ACCESSION   DBNGOH010000010 DBNGOH010000000
VERSION     DBNGOH010000010.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3443)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3443)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3443
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1650887"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..112)
                     /locus_tag="AC159P_00215"
                     /pseudo
     CDS             complement(1..112)
                     /locus_tag="AC159P_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012632316.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming cytochrome c nitrite reductase
                     subunit c552"
     gene            439..1293
                     /locus_tag="AC159P_00220"
     CDS             439..1293
                     /locus_tag="AC159P_00220"
                     /inference="COORDINATES: protein motif:HMM:NF025615.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwiC-like family protein"
                     /protein_id="HIR3693902.1"
                     /translation="MTPSSFVNEFRETDSAHAVGRVARVRLRSVALPVEHGGWGLLLE
                     PIALGLLVAPSLTGFCLSFAAAGAFLARHPFKIVIADLRRRRRFPRTAAAERFALLYG
                     FISVAGMIGAAATAADFKFLLPLLAATPFAATQLFNDVKGRSRSLIAETTGAVALAAL
                     SASVALAGGWEIPAAVALWAVLAARAVPAILYVRARIAELHGETPSVAPSLFLHGLAL
                     VAVLLLTLLGVASGLALLAMCVLLLRAVHGFSAPQQAATARQIGVREIGFGALTVFAV
                     AAGHLLRW"
     gene            complement(1455..1622)
                     /locus_tag="AC159P_00225"
                     /pseudo
     CDS             complement(1455..1622)
                     /locus_tag="AC159P_00225"
                     /inference="COORDINATES: protein motif:HMM:NF039641.6"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6064 family protein"
     gene            complement(1629..2579)
                     /locus_tag="AC159P_00230"
     CDS             complement(1629..2579)
                     /locus_tag="AC159P_00230"
                     /inference="COORDINATES: protein motif:HMM:NF012791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR3693903.1"
                     /translation="MKILIAYDGSPCAEAALDDLRRAGLPAAAEAYVISVTEVWLPPP
                     PPSVYEVVEEATTAHTPAELQMEYVKESPSVEEARLLAAGARNRLLDNFPGWVVRAEG
                     RYGSPAREIVRLADEWKPDLVVVGSQGHTALGRLVLGSVSQKVVTEARNSVRVARGRV
                     EVEPKPVRVVVGVDGSPGSEAAVREVAARCWPPGGEARVVVVDDPLVPSPFGQLIPPV
                     TRWVEESNREERAWVQEMADEAASRLRRAELVVSSSVVVGDPKRALVEEAERWGADCI
                     FVGSHGFGNRLERFLLGSVSAAVAARAHCSVEVVRAPKDE"
     gene            complement(2597..3115)
                     /locus_tag="AC159P_00235"
     CDS             complement(2597..3115)
                     /locus_tag="AC159P_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010940934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR3693904.1"
                     /translation="MKILLAVDGSTFGDAAVSEVARRPWPAGSEVRIISAAEASHIPA
                     PVPHGAADLYAEALEDACEQARTALARAASKLSAEGGTGLRITTAAPVGPAKEVILSE
                     AEEWGADLIVVGSHGRGFWKRLFLGSVSQAVAAHAPCSVEIVRGPKAAEADSTNGAEG
                     SAVSAAAEAARE"
BASE COUNT          520 a         1246 c         1075 g          602 t
ORIGIN      
        1 agcccgtcga gttctcggct tcgacgaagt cgaggtagaa ctgcgccttg cgctggaagt
       61 cgcgggccgt ctgtagctcg gcgtcggtgc gcccggcggc gcgcgccgcc ttgtgagggg
      121 acaaaactta tatcgaagtc tgtgccgcac cacttccgaa cgggcgcgga gaaaaacttg
      181 cgaccggcga ccggcacgcc ttcatgcctt gcgcctcgcc cgccgcgagt gaaaatttcc
      241 ccaatgcctc agcggcgact gcgtgagatt tcgcaacgcg cacgccaccg gccacccccg
      301 cgccgccttc ttccgaaaac attcacgccg tccttcagtg gccctgcgct tgcacggcgc
      361 atgtcgtggg acgatgggaa ccgcccccga gccatgcggc ttcggacatg accacgcgat
      421 cagttttgag tgaatagcat gaccccttca tcgttcgtca acgagtttcg agaaaccgac
      481 tcggcacacg ccgtggggcg agtcgcccgc gtgcgcttgc gctccgtcgc cctgccggtc
      541 gagcacggcg gttggggtct cttgctcgaa ccaatcgcgc tcggccttct ggtcgcgccg
      601 tcactgacgg gcttttgcct ctcgttcgcg gcggcgggtg cgttcctcgc gcgccacccg
      661 ttcaagatcg tgatcgccga ccttcgacgg cggcggcgct tcccgcgcac cgccgcggcg
      721 gaacggttcg cgctgctcta cggattcatc tccgtcgccg gtatgatcgg cgccgcggcg
      781 accgcagccg acttcaagtt tcttctcccg ctgctcgcgg caacgccctt cgcggcgact
      841 cagttattca atgacgtgaa gggacgcagc cgctcactca tcgcggagac gaccggggcg
      901 gtggctctcg ccgcgctgtc ggccagcgta gccctcgcgg gcgggtggga gattccggcg
      961 gcagtcgcgc tgtgggcggt gctcgcggcc cgggccgtac cggcgatcct gtacgtgcgc
     1021 gcgcgcatcg cggaactgca cggcgagacg ccgtcggtcg cgccttcact ctttctgcac
     1081 gggctggcgc tcgtggccgt actgctgctc acgctgctcg gcgtcgcgtc aggtctcgcc
     1141 ctgctcgcga tgtgcgtcct cctgctacgc gccgtccacg gcttttccgc gccgcagcag
     1201 gcggcgaccg ccaggcagat cggcgtccgc gaaatcggct tcggcgcgtt gacggtcttc
     1261 gccgtcgccg cgggacatct gctcaggtgg tagggatcga aagacgcggc ggtacgcaca
     1321 cacggtctac atataggttt cgattccgca aggcaatgag cgtcgcgccg atcaccccgg
     1381 cggctaacag cccgaagtct tcggtcatgc ccaaatacaa cgcagccgtc gttccgatta
     1441 tcgaccagcc aaaacagcgc caacgccccg ctgattatcc tgtccgaaga cgggcgcttt
     1501 cttaccgcta gaagcaacgt cagtccggcg acggagttga agacgagttg cagcggccac
     1561 accgcgcggt tgtactcggc gaagacccgc aggaactgct cctcgctgaa cggcagggcc
     1621 atctcaccct attcgtcctt cggcgcgcgg acgacttcga ccgagcagtg cgcgcgggcc
     1681 gccaccgccg ccgacacgct ccccagcagg aagcgctcca gccggttgcc gaagccgtgc
     1741 gagccgacga agatgcagtc ggccccccac ctttcggcct cctcgacgag cgcacgcttg
     1801 gggtctccaa cgacgacgga tgacgagacc acgagttccg cccggcgcag tcggcttgcc
     1861 gcctcgtcgg ccatctcctg cacccacgcg cgctcctcgc ggttgctctc ttcgacccac
     1921 ctcgtcacgg gcgggatgag ctgaccgaac ggactcggca cgagcgggtc gtccacgacg
     1981 acgacgcgcg cctcgccccc aggcggccag cagcgcgccg ccacctcgcg caccgcggcc
     2041 tccgagccgg gcgagccgtc cacgccgacg actacacgca ccggcttcgg ctcgacctcg
     2101 acgcgaccac gcgccacgcg cacggaattg cgcgcctccg tcacgacctt ctgcgacacg
     2161 ctgccgagga cgagtctgcc gagagccgtg tggccctgcg acccgacgac gacgaggtcg
     2221 ggcttccact cgtccgccag cctgacgatt tcgcgtgcgg gcgagccgta gcgtccctcg
     2281 gcccgcacca cccatccggg gaagttatcg aggagccggt tcctcgcccc cgcggcgagg
     2341 aggcgcgcct cttcgaccga gggcgattct ttgacgtact ccatttggag ttcggcgggc
     2401 gtgtgcgccg tcgtcgcctc ttcgaccact tcgtagacgg acggcggggg cggcggcagc
     2461 cagacctccg tgaccgaaat cacgtaagcc tcggctgcgg cgggcagccc cgcccgtcgc
     2521 aggtcgtcga gcgccgcctc ggcgcacggc gagccgtcgt aagcgatcaa tattttcata
     2581 acgctcttca tggcatctac tcccgagccg cttccgccgc ggcgctgacg gctgacccct
     2641 ccgcgccatt ggtcgaatcc gcctcagccg ccttcgggcc gcgtacgatc tcgacggagc
     2701 acggggcgtg cgcggcgacg gcttgcgaga ccgagcctaa aaatagccgc ttccagaatc
     2761 cgcgcccgtg cgagccgacc acgatcaaat ccgcgcccca ctcttcggcc tcactcagga
     2821 tgacctcctt agcggggccg accggtgcag ccgtcgtgat tcgcagcccc gtgccccctt
     2881 cggcgctcag tttcgaagcc gcgcgagcaa gcgctgtgcg cgcttgctcg caggcgtctt
     2941 ccagagcttc ggcgtagagg tctgcggcgc cgtgcgggac gggcgccggg atgtgtgacg
     3001 cttcggcggc ggagatgatt cgcacctcgc tccccgcggg ccacggtcgc cgcgccacct
     3061 cgctcaccgc cgcgtcgccg aacgtcgagc cgtctactgc gagtagtatt ttcatgaccc
     3121 acctccgatt agttgacaac ggggccgcag gataatcacc gcttcatcct tatgctctct
     3181 ttcgcccctc acgtctccca ccgagtcgag tgcaacgccc gtaccagcgg aagtaattcc
     3241 acaccctcgt tcaatcgtgc aggttccgtt cgaggcgcgc cctgtcgtgc gtgttttcac
     3301 gccggggcgt gcgtaagttt ccgcacggcg gggcggagac gtgtacccct gcccctaagt
     3361 cgcctgccaa agcttcaggc atatccgttg ctgtcggtaa agccgtgtgg tctcgtgagg
     3421 ctacgcctcg ttgacggttc gag
//
LOCUS       DBNGOH010000011         2041 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_734552, whole genome shotgun sequence.
ACCESSION   DBNGOH010000011 DBNGOH010000000
VERSION     DBNGOH010000011.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2041)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2041)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2041
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_734552"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2018)
                     /locus_tag="AC159P_00240"
     CDS             complement(<1..2018)
                     /locus_tag="AC159P_00240"
                     /inference="COORDINATES: protein motif:HMM:NF016348.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS-assembly protein LptD"
                     /protein_id="HIR3693905.1"
                     /translation="MQSTEGLDVRSGKQTVSGPENARVFVHEGNVDARVGIYRLQADK
                     VTVYEATNMVFAEGNVVFDQGQFQRITGSRAEWNYATKIGFFVNATGFSNQTDDGTII
                     YFTADRVEKIAGNKVVVLEGEITACGDDEVPKWSFHTKRAVITLNDRVRGKNASFRIK
                     GVPVGYLPFFSVSIKPRDRASGFLTPTFSGSGKKGFRISNAYYQTLGRSADITFSNDI
                     FTGRGLGIGADLRTRANSRSYLNVGFYAVKDRIFGAEAGPNDPDQGGSSFYVDGVHYF
                     PNGFLAAADVNITSSLDFRGIFSDSIQQAISPEERSQVFVNKNSGDYSLNFIARTQVV
                     SIPSVRVRTRALPGVSLDKRPSELPFVEKLPLYFSFEGSVEGVSRKETVTDLFTFNAE
                     GNANPIITPSIVQRLDFHPKFSVPFDLPGGWGVTATAKLRGTFYSNSLEPPTRLVLAR
                     DVVRGYGEFELDVRPPALARNFRRGEENEFWFRHVVEPYFIYRKIGGISNFERLIRFD
                     STDAVADTNEFEFGLTNRFFTRRSTESVTGRVSRYGRGGGLGGTRGEEERDEREERRR
                     EEERRRDENRRDDEQQTPEGEARQTTAAGEDTRQPLARQPYEFLSITLRQKYFFDRTF
                     GGALVAGRRNQFYPINTFSGFTYGGVPRRFSPLNVEARLRTPTRADSEL"
BASE COUNT          337 a          637 c          720 g          347 t
ORIGIN      
        1 agctccgagt cggcgcgcgt cggcgtgcgc aggcgcgcct cgacgttcag gggcgagaag
       61 cggcgcggca cgccgccgta ggtgaagccc gagaaggtgt tgatcgggta gaactgattg
      121 cgccgaccgg cgacgagcgc gccgccgaac gtgcggtcga agaaatactt ctgccgcagc
      181 gtgatcgaca agaactcgta gggctggcgc gcgagcggct gccgcgtgtc ttcgcccgcg
      241 gcggtagtct gccgcgcctc gccctccggc gtctgctgtt cgtcgtcacg acgattctcg
      301 tcacgacgcc tctcttcctc gcgccggcgc tcctcgcgct cgtcgcgctc ctcttcgccg
      361 cgcgtcccgc ctaatccgcc gcccctgccg tagcgcgaga cgcggcccgt cacgctctcg
      421 gtcgagcggc gcgtgaagaa gcggttggtg aggccgaact cgaactcgtt ggtgtcggcg
      481 acggcgtcgg tcgagtcgaa gcggatgagg cgctcgaagt tcgagatgcc gccgatcttg
      541 cggtagatga agtaaggctc gacgacgtgg cggaaccaga actcgttctc ctcgccgcgg
      601 cggaagttgc gcgcgagcgc ggggggccgc acgtccagct cgaactcgcc gtagccgcgc
      661 accacgtcgc gggcgaggac aaggcgcgtc ggcggctcca gagagttcga atagaaggtg
      721 ccacgcagct tcgccgtcgc cgtcacgccc cagccgcccg gcaggtcgaa cggcacgctg
      781 aacttcggat gaaagtcgag gcgctgcacg atcgaaggcg tgatgatcgg gttggcgttg
      841 ccctcggcgt tgaaggtgaa gaggtcggtg acggtctcct tgcggctgac gccctcgacg
      901 ctcccctcga acgaaaagta gagcggcagc ttttcgacga acggaagctc ggagggccgc
      961 ttgtcgagcg agacgccggg cagagcgcgc gtgcgcaccc tgacggaagg gatcgagacg
     1021 acctgcgtgc gcgcgatgaa gttgaggctg tagtcgcccg agttcttgtt gacgaagacc
     1081 tgcgagcgct cctcgggcga gatggcctgc tggatcgagt cggagaagat gccgcgaaag
     1141 tcgaggctcg aagtgatgtt cacgtccgcc gccgccagaa agccgttggg gaagtaatga
     1201 acaccatcga cgtagaacga agagccgccc tgatccgggt cgttcggccc ggcctcggcg
     1261 ccgaagatgc gatccttgac ggcgtagaaa ccgacgttga ggtacgagcg cgagttggca
     1321 cgcgtgcgca aatccgcccc gatgccaagc ccccggcccg tgaagatgtc gttgctgaag
     1381 gtgatgtccg ccgagcgccc gagcgtctgg tagtaggcgt tcgagatgcg gaagcccttc
     1441 ttgccggagc cggagaaggt cggcgtcagg aagcccgagg cgcggtcgcg cggcttgatc
     1501 gagacggaga agaacggcag gtaaccgacg gggacgccct tgatgcggaa cgaggcgttc
     1561 ttgccgcgca cgcggtcgtt gagggtgatg acggcgcgct tcgtgtggaa gctccacttc
     1621 ggcacctcgt cgtcgccgca ggccgtgatc tcgccttcga gcacgaccac cttgttgccc
     1681 gcgatcttct cgacgcggtc ggcggtgaag tagatgatgg tgccgtcgtc ggtctggttc
     1741 gagaagccgg tggcgttgac gaagaagccg atcttggtcg cgtagttcca ctcggcgcgc
     1801 gagccggtga tgcgctggaa ctgcccctga tcgaagacga cgttgccctc tgcgaagacc
     1861 atgttcgtcg cctcgtagac cgtcaccttg tcggcctgga ggcggtagat gccgacgcgc
     1921 gcgtcaacgt tcccctcgtg gacgaagacg cgcgcgtttt cggggccgct cacggtctgc
     1981 ttgcccgagc gcacgtcgag tccttccgtg gactgcatcc cgccctcgat ctcggcgggc
     2041 c
//
LOCUS       DBNGOH010000012         3066 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2017983, whole genome shotgun sequence.
ACCESSION   DBNGOH010000012 DBNGOH010000000
VERSION     DBNGOH010000012.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3066)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3066)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3066
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2017983"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..154
                     /locus_tag="AC159P_00245"
     CDS             <1..154
                     /locus_tag="AC159P_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018791606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3693906.1"
                     /translation="AMQFNAVVESNESAVRLWQALGFRIIGTVPEAFAHPTLGRVGLH
                     VMHRRL"
     gene            185..1099
                     /locus_tag="AC159P_00250"
     CDS             185..1099
                     /locus_tag="AC159P_00250"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF015000.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HIR3693907.1"
                     /translation="MARRRAIKLMAAVGLGGLAVLGALYGALASADGERAADHPFFAR
                     DGGGPLVVAHRGGAGLWPENTLHAFERAFELGVDVVELDVRSTADGALAVIHDPTVER
                     TTDGQGRVEALTLAELQGLDAGHRFSADGGQTFPLRGRGLRVPTLGEVFERLPRARFI
                     IEMKDESQRTAKSLCETVARRGMGERVVVGSFRQAMLDEFRRACPGVATSASTLEASK
                     FLALYRAGLAASYSPPMQALQVPENAGGMAVLTRGFVEAAHGRNLEVHAWTINDPADM
                     RRLLDEGVDGIMTDYPDRLLAMLKRGKG"
     gene            1369..>3066
                     /locus_tag="AC159P_00255"
     CDS             1369..>3066
                     /locus_tag="AC159P_00255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M16 family protein"
                     /protein_id="HIR3693908.1"
                     /translation="MNKKRPSLLALAMLSLSLLTGAMPRAAMARQVDGVALGALTEGK
                     SVNGFRAEALYLNDSDQPFGARLRHERTGFTLDYMEMQSVPQMFLWVNSFPTADHGEP
                     HTQEHLLLGKGNVGRAHAGFESMSLAGSSAFTMQWRTAYHMHTAAGADIFYKLFESQV
                     GALLHPDYTDEEIRREVSHWGVSENPADKKLKLEEKGTVYQEMASTYERPVSLLFREI
                     NQMLYGDAHPLALSAGGWPSSIRAMKPEHIRKFHADNYQLGNMGMVASFPKEMPLASV
                     LTRLDQILNRLEPKQEKRAFPSEADLPAPKAAAEGQIELVEYPHKNEQQPGWVMFAWP
                     AQLKLDTREKTLLNLFLANVASDPTTNLYKLFVDTKTRVMDTGAKGVFAFLDDEMVVG
                     NPVYVGLTDVQPAHMTQENIANIRQRIMDEMKRVASFADGSPELSEFNARLKSRVIQQ
                     RRTLSKFVNSPPGFGFRNSDSGWMTHLQDLNRTAGFRKSVTLKPELAEVDKLLDAKVN
                     AWRTLVARWKLADTKPYAVAAKPAPQLIARTEQEAAARAAAELARLKTKYNATDEQEA
                     "
BASE COUNT          549 a         1050 c         1017 g          450 t
ORIGIN      
        1 cgcgatgcag ttcaacgccg tggtggaatc gaacgagagt gccgtgcgac tgtggcaggc
       61 gctcgggttt cggatcatcg ggactgtccc cgaggcgttc gcgcacccca cgctcgggcg
      121 cgtcggcctt cacgtcatgc accgacgcct ctaggatgcg acgacgcggc gggaaggaat
      181 gaacgtggcg agaaggcgtg cgataaagtt gatggcggct gtaggactcg gcggactcgc
      241 cgtgctcggg gcgctctacg gcgcgctggc gagtgcggac ggggagcgcg cggcggatca
      301 cccgttcttc gcgcgcgacg gcggcggccc gctcgtcgtc gcgcaccgcg gcggggcggg
      361 gctgtggccc gagaacacgc tgcacgcctt cgagcgcgcg ttcgagctgg gcgtggacgt
      421 ggtcgaactc gacgtgcgtt cgaccgccga cggcgcgctc gccgtcatcc acgacccgac
      481 cgtcgagcgc acgaccgacg ggcagggccg cgtcgaggcg ctcacgctcg ccgagttgca
      541 ggggctggac gccggccacc gcttcagcgc cgacggcggc cagacctttc ctttgcgcgg
      601 gcggggcttg cgcgtgccga cgctcggcga agtgttcgag cggctgccgc gcgcgcgctt
      661 catcatcgag atgaaggacg agtcgcagag gacggccaag agcctgtgcg agacggtggc
      721 gcggcgcggg atgggcgagc gcgtcgtcgt cggctcgttc cggcaggcga tgctcgacga
      781 gttccgccgc gcgtgccccg gcgtggcgac ctcggccagc acgcttgagg cgagcaagtt
      841 cctcgcgctc tacagggccg ggctggccgc gagctacagc cccccgatgc aggcgctcca
      901 ggtgcccgag aacgcgggcg ggatggcggt gctcacgcgc ggcttcgtcg aggccgcgca
      961 cgggcgcaac ctcgaagtcc acgcctggac gatcaacgac cccgcagaca tgcgccgcct
     1021 cctcgacgag ggcgtcgatg gcatcatgac cgactatccc gaccgcctgc tcgcaatgtt
     1081 gaagagggga aaaggttaaa aggggaagaa gcgaaaaggg aatgaaggat gatgcgagat
     1141 atgctgtttc ttatcccctc ttcccccttt ccccttttaa ccttttcccc ttctttccga
     1201 ttgacctacg cggccctcgc aggtagcatc gaaggctctc cgcacttccg ttgtagtcgc
     1261 aaggaaaatt gaatttcatc cgaaaaatcg tacgccggga aacgaccgcc cgcggccctc
     1321 tcaagacgcg tggcgccgcc gccggcgtcc acacgacagg agtctccgat gaacaagaaa
     1381 cgcccttccc tgctcgcact cgcgatgctc tcactctccc tgctgacggg cgcgatgccg
     1441 cgcgcggcga tggcgcggca agttgacggc gtcgcgctcg gcgcgctgac cgagggcaag
     1501 tccgtgaacg gcttccgcgc cgaggcgctc tacctcaacg actccgacca gccgttcggc
     1561 gcgcgcctcc ggcacgagcg caccggcttc acgctcgact acatggagat gcagtcggtg
     1621 ccgcagatgt tcctgtgggt caactcgttc ccaacggcag accacggcga gccgcacacg
     1681 caggagcacc tgctcttagg caagggcaac gtcgggcgcg cgcacgccgg cttcgagagc
     1741 atgtcgctcg ccggctcttc ggccttcacg atgcagtggc gcacggccta ccacatgcac
     1801 accgccgccg gagccgacat cttctacaag ctcttcgaga gccaggtggg cgcgctgctg
     1861 cacccggact acacggacga ggagatacgc cgcgaggtga gccactgggg cgtcagcgag
     1921 aaccccgcgg acaagaagct caagctcgaa gagaaaggca ccgtctatca ggagatggcc
     1981 tcgacctacg agcggcccgt cagcctgctc ttccgcgaaa tcaaccagat gctctacggc
     2041 gacgcgcacc cgctcgcgct gtcggcgggc ggctggccct cctcgatccg cgcgatgaag
     2101 cccgagcaca tccgcaaatt ccacgcggac aactaccagc tcggcaacat gggcatggtc
     2161 gcctcgttcc cgaaggagat gccgctcgcg tccgtgctca cgcggctcga ccagattctg
     2221 aaccggctgg agccgaagca ggagaagcgc gccttcccct ccgaagcgga tctgcccgcg
     2281 ccgaaggccg cagccgaggg gcagatcgag ctggtcgagt acccgcacaa gaacgagcag
     2341 cagccgggct gggtgatgtt cgcgtggccc gcgcagctca agctcgacac gcgcgagaag
     2401 acgctcctca acctcttcct cgcgaacgtg gcgagcgacc cgacgaccaa cctctacaag
     2461 ctcttcgtgg acacgaagac gcgcgtcatg gacacgggcg cgaagggcgt cttcgccttc
     2521 ctcgacgacg agatggtcgt cggcaacccc gtctacgtcg gcctgacgga cgtgcagccg
     2581 gcgcacatga cgcaggagaa tatcgccaac atccgccagc ggatcatgga cgagatgaag
     2641 cgcgtcgcct ctttcgccga cggctcgccg gagctttcgg agttcaacgc gcgcctcaag
     2701 agccgcgtca tccagcagcg ccgcacgctc tcgaagttcg tcaactcgcc gccgggcttc
     2761 ggcttccgta actcggactc gggctggatg acgcacctgc aagacctcaa ccgcacggcg
     2821 ggcttccgca agtcggtcac gctcaagccg gaactcgcgg aagtcgataa gctgctcgac
     2881 gcgaaagtga acgcgtggcg cacgctcgtc gcgcgctgga agctggccga cacgaagccc
     2941 tacgccgtgg ccgcgaagcc cgcgccgcag ctcatcgcgc gcacggagca ggaggccgcg
     3001 gcgcgcgccg ccgccgaact ggcgcggctc aagaccaagt acaacgcgac ggacgagcag
     3061 gaggcg
//
LOCUS       DBNGOH010000013         5988 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4400702, whole genome shotgun sequence.
ACCESSION   DBNGOH010000013 DBNGOH010000000
VERSION     DBNGOH010000013.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5988)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5988)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5988
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4400702"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2042
                     /locus_tag="AC159P_00260"
     CDS             <1..2042
                     /locus_tag="AC159P_00260"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004766 - spermidine synthase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="spermidine synthase"
                     /protein_id="HIR3693909.1"
                     /translation="RGVPEDGIPALWLLGRLFLMVGLPFFAVSTSAPLLQKWFSRTRH
                     ASADDPYFLYSASNMGSFFALLGFPLLVEPNWTLGAQGRYWAWGYGVLALLILACAFA
                     SRRGDETAAASSSTTGGAIDAGDAGEKVDGSAAAETDAGEVSWRRRLRWTLLAFVPSS
                     LVLGVTTYITTDVAAVPLLWVIPLSIYLLTFVLVFARRQIVPWGWMATILPGSALLLA
                     LIYLSGATEPVWFLVLVHLIFLFVASMVCHGQLAADRPDARHLAEFYLWVAVGGVLGG
                     FFNAIVAPLVFNAVAEYPLMVVLACALRPTPARRTLVITRKGLKVKNAAPDETKVVDE
                     ATALRNDLVFPLAIFALAAALVVYVPRLGLKPVEDVAIMYGVPLLLLNHFFARRPFRF
                     ALGLGAVMLAVVFFSGTRARTLHAERNFYGTLRVVEDSANDMRRLLHGSTLHGRQWTD
                     DQRRCEPLSYYHREGPLGAVIETYRARPASPNVAAVGLGAGASIAHSRAGERWTFYEI
                     DPAVDDIARDPNYFTYLTYCSAAPNRTVLGDARLKLREAPDGHYGLVILDAFSSDAIP
                     AHLLTREALRLYLSKLAPGGLIAFHVSNRALNLDAVVGGLARDAGLEARIFNDHDYRP
                     EIGKEPSHWAVLVRRPEDLGALAADARFVPLDESRHKFEIWSDDFSDIISVFKWF"
     gene            2162..2629
                     /locus_tag="AC159P_00265"
     CDS             2162..2629
                     /locus_tag="AC159P_00265"
                     /inference="COORDINATES: protein motif:HMM:NF039314.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5991 domain-containing protein"
                     /protein_id="HIR3693910.1"
                     /translation="MKRALALCAAFILLPVLPSHVAAGTQGPPRDEWAGTYEYTASLG
                     RTAGGRGIVVTYTIEVTSGNSELGAVVEASGYMTDDKLRCETRARGNRIDLHFHSYPD
                     GGTANAYGVELYKKGDLLLSLEKVVQQKRTRYRVIWAKYNTDFKKRVFFRKTA"
     gene            2725..4632
                     /gene="selB"
                     /locus_tag="AC159P_00270"
     CDS             2725..4632
                     /gene="selB"
                     /locus_tag="AC159P_00270"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HIR3693911.1"
                     /translation="MRSIIVGTAGHIDHGKTSLVRALTGVDADRLPEEKRRGITIDLG
                     FAELDLGEVRVGFVDVPGHERFVKNMLAGAHGVDLVALVVAADEGVMPQTREHFDICR
                     LLGVGAGLVAVTKRDAVEEELLELVRAEIEELVAGSFLEGAPVVAVSSLTGAGLEEFK
                     TTLRELSLKAPPRASDSVARLPVDRAFTMRGFGAVVTGTLVAGEIREGEEMELLPAGE
                     RVRVRGVQVHGSAVRAARAGQRTAVNLGGVETASIERGMVLAPAGRLQPTQIIDAHVE
                     VLRTAPRPLRSRQRVRVHVGAAEVLARVSVLEDEGEVVPGASGLVQLRLEAPVVALPG
                     ERFIVRSYSPQQTVAGGEVVDAHAAKHRGRERRTARARLASLAGAPPAARAAYFVETA
                     GEKGLRRAELASRTGWRDEVLDAALREAAGDGAVVEAEGVYVSRGVFDELLRRTLAEL
                     EAHHAREPLARGMARETLRERLFARAAPEVFRAVMREGEGRGVLVSERDVVRASTHSL
                     DLSPEDAAARDLLEKTYREAALEAPALEEALAAAGPTRERARKLLQLLLDAGTLVRVR
                     EDLLFHRDALAALTVALRDYGARHEPERLIDVAAFKELSGVSRKYAIPLLEYFDRERV
                     TRRAGDKRLIL"
     gene            complement(4780..>5988)
                     /locus_tag="AC159P_00275"
     CDS             complement(4780..>5988)
                     /locus_tag="AC159P_00275"
                     /inference="COORDINATES: protein motif:HMM:NF015140.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007154 - cell communication [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Calx-beta domain-containing protein"
                     /protein_id="HIR3693912.1"
                     /translation="VVVFTSEASNLDPRDTDTESDVFSVRLAGQVQLSAETFTADEGG
                     GAVNVTVTRTGDTSGETFVNFATTDGAASDRRDYTAALGRLRFAPGETSKSFQIVITD
                     DRFDDDGESFNVVLTGPTGATLGAPANATVNIIDNDAAGGPSPVKDASFNTEFFVRQH
                     YADFLGRVPDAGGLAFWIDNIESCGTNLQCREVRKVDTSAAFFLSIEFQETGFLAYRT
                     HKAAFGDLAGKPVPIRLRDFLFDTRQIGDGVVVGAPGWPEQLEANKAAYFNAFVARTA
                     FSTLYPQSMTAALFVDALDTRAGNPLSQAERDALVSDLTNGTKTRAQVLRAVAEDETL
                     RQAETNKAFVLMQYFGYLRRNPDDPQDTNFDGFDFWLLKLNQFGGNYVEAEMVKAFIQ
                     SIEYGDRFGF"
BASE COUNT          856 a         2045 c         2113 g          974 t
ORIGIN      
        1 cgcgcggcgt gcccgaagac ggcatcccgg cgctgtggct gctgggccgc ctgttcctga
       61 tggtgggcct gccgttcttc gccgtctcga ccagcgcgcc gctcttgcag aagtggttct
      121 cgaggacgcg ccacgcgtcg gcggacgacc cctacttcct ctactcggcg agcaacatgg
      181 ggagcttctt cgcgctgctg ggcttcccgc tgctggtcga gccgaactgg acgctcggcg
      241 cgcaagggcg ctactgggcg tggggctacg gcgtgctcgc gctgctcatc ctcgcctgcg
      301 ccttcgcctc gcgccgcggc gacgagacgg cggcggcgtc gtcgagcacg acgggcgggg
      361 cgatcgacgc gggcgacgcc ggcgagaagg tcgatgggag cgccgctgcc gagacggacg
      421 ccggggaggt gtcttggcga cggcggctgc gctggacgct gctcgcgttc gtgccttcga
      481 gcctcgtgct cggcgtgacg acctacatca cgacggacgt ggccgccgtg ccgctgctct
      541 gggtcatccc gctctcgatc tatctgttga ccttcgtgct cgtcttcgcc cggcggcaga
      601 tcgtcccgtg gggttggatg gcgacgatcc tgccggggtc ggcgctgctg ctggcgctca
      661 tctatctttc gggcgcgacc gagccggtgt ggttcctcgt cctcgtccac ctcatcttcc
      721 tcttcgtcgc ctcgatggtc tgccacgggc agttggccgc tgaccgaccg gacgcgcgcc
      781 acctcgcgga gttctacctg tgggtcgcgg tgggcggcgt gctcggcggg ttcttcaacg
      841 ccatcgtcgc gccgctcgtc ttcaacgcgg tcgccgagta tccgctgatg gtcgtgctcg
      901 cctgcgcgct ccggcccacg cccgcgcgcc gcacgctcgt catcacgcgc aaaggtctca
      961 aggtgaagaa cgcggcgccg gacgagacga aggtcgttga cgaagcgacg gcgctgcgca
     1021 acgacctcgt gttcccgctc gccatcttcg cgctcgccgc ggcgctcgtc gtctacgtcc
     1081 cgcgcctcgg cctcaagccg gtcgaggacg tggccatcat gtacggcgtt ccgctgctcc
     1141 tgctcaacca cttcttcgcg cggcgcccct ttcgcttcgc gctcggcctc ggcgcggtga
     1201 tgctcgcggt cgtcttcttc tccggcacgc gcgcgcgcac cctgcacgcc gagcggaact
     1261 tctacggcac gctgcgcgtc gtcgaagact ccgcgaacga catgcgccgc ctgctccacg
     1321 gcagcacgct gcacgggcgg cagtggacgg acgaccaaag gcgctgcgag ccgctctcgt
     1381 actaccaccg cgaggggccg ctcggcgccg tcatcgagac ctaccgcgcg cgccccgcct
     1441 cgcccaacgt cgcggcggtc gggttaggtg cgggcgccag catcgcgcac tcgcgcgcgg
     1501 gcgagcgctg gacgttctac gagatcgacc cggcggtcga tgacatcgcg cgcgacccga
     1561 actatttcac ctacctcacc tactgctccg ccgcgcccaa ccggacggtg ctcggcgacg
     1621 cgcgcctgaa gctgcgcgaa gcgcccgacg ggcactacgg cctcgtcatc ctcgacgcct
     1681 tcagctccga cgccatcccc gcgcacctgc tgacgcgcga ggcgctcagg ctctacctct
     1741 ccaagctcgc gcccggcggc ctcatcgcct tccacgtctc gaaccgcgcg ctcaacctcg
     1801 acgccgtcgt cggcggcctc gcgcgcgacg ccggcctcga agcacgcatc ttcaacgacc
     1861 acgactaccg gcccgagatc ggcaaagagc cttcgcactg ggccgtcctc gtccgccgac
     1921 ccgaagacct cggcgcgctg gccgcggacg cacgcttcgt cccgctcgac gagagccggc
     1981 acaagttcga aatctggagc gatgacttct ccgacatcat cagcgtcttc aagtggttct
     2041 gacgcgccgg ggacggcgga taaaacagga gcacggcttt tttaagccgt gctcctgtgc
     2101 ctttgtggta agttcctgcc cagtcccatt cgagaccgag gagaccacgg gaggcacgtt
     2161 catgaaaaga gcactcgccc tctgcgccgc cttcatcctt ttgcccgtgc tgccgtcgca
     2221 cgtcgcggcg ggcacgcaag gcccgccccg cgacgagtgg gcgggcacgt acgagtacac
     2281 ggccagcctc ggtcgcaccg cgggcgggag gggcatcgtc gtcacctaca ccatcgaagt
     2341 cacgtcgggc aactccgagc tgggggccgt cgtcgaggcg agcgggtaca tgaccgacga
     2401 caagctcagg tgcgagacgc gggcgcgggg caaccgcatc gacctccact tccacagcta
     2461 cccggacggc ggaaccgcca acgcgtacgg ggtggagttg tacaagaagg gcgacctcct
     2521 gctctcgctc gaaaaggtcg ttcagcaaaa gcggacgaga tacagggtga tctgggcgaa
     2581 gtacaatacc gatttcaaga agcgcgtctt cttcaggaag accgcttagc cggtgcatgg
     2641 taaatggtga atggtgaata gagagtgaca cgctttctgt tcaccattca ccatttacca
     2701 ttcaccattt accatgcacc aatcatgcga tccatcatcg tcggcaccgc cgggcacatc
     2761 gatcacggga agacctctct cgtccgcgcc ttgacgggcg tggacgccga ccgcctgccc
     2821 gaagagaagc ggcggggcat cacgatcgac ctcggcttcg ccgaactcga cctcggcgag
     2881 gtgcgcgtcg ggttcgtgga cgtgccgggc cacgagcgct tcgtgaagaa catgctggcc
     2941 ggggcgcacg gcgtcgatct ggtggcgctc gtggtggcgg cggacgaggg cgtgatgccg
     3001 cagacgcgcg agcacttcga catctgtcgc ctgctcggcg tcggcgccgg tctggtcgcc
     3061 gtgacgaaac gggacgcggt cgaagaggaa ctgctcgaac tcgtccgcgc agagatcgaa
     3121 gaactggttg caggctcctt cctcgaaggc gcgccggtgg tggccgtcag ctcgttgacg
     3181 ggcgcggggc tggaggagtt caagacgacc ctgcgcgagt tgtccctgaa ggcgccgccg
     3241 cgcgcttcgg actcggtggc gcggctgccg gttgaccgcg ccttcacgat gcgcggcttc
     3301 ggcgcggtgg tgacgggcac gctcgtcgcg ggcgagatca gggaaggcga agagatggag
     3361 ctgctgcccg cgggcgagcg cgtgcgcgtg cgcggcgtgc aggtgcacgg ctcggcggtg
     3421 cgtgcggcgc gcgcggggca gcgcacggcg gtcaacctcg gcggcgtcga gaccgcttca
     3481 atcgagcgcg ggatggtgct cgcgcccgcc gggcgccttc agccgacgca gatcatcgac
     3541 gcacacgtag aggtgttgcg caccgcgccg cgccctttgc gctcgcgcca gcgcgtgcgc
     3601 gtccacgtcg gcgcggcgga ggtgctggcg cgcgtctcgg tgttggagga cgagggcgag
     3661 gtcgtgccgg gcgcgtcggg cctcgtgcaa ctgcggctcg aagcgccggt ggtcgcgctg
     3721 cccggcgagc gcttcatcgt ccgctcctac tcgccgcagc agacggtcgc gggcggcgaa
     3781 gtcgtggacg cgcacgcggc gaagcaccgc gggcgcgaac gtcgcacggc gcgcgcgcgg
     3841 ctcgcctcgc tcgcgggcgc gccgcccgcc gcgcgcgcgg cttacttcgt cgagactgcg
     3901 ggcgagaagg gcctgcggcg cgcggagctg gcgtcgcgca cgggctggcg cgacgaggtg
     3961 ctggacgccg cgctccggga agccgcgggg gacggcgcgg tcgtcgaggc cgagggcgtg
     4021 tacgtgagcc gcggggtctt cgacgagctg ctgcggagga cgctggccga gcttgaggcg
     4081 caccacgcgc gcgagccttt ggcgcgcggg atggcgcgcg agaccttgcg cgagcgcctg
     4141 ttcgcgcgcg ccgcgcccga ggtcttccgc gccgtgatgc gcgaaggcga agggcgcggc
     4201 gtgctggtct ccgagcggga cgtggtgcgc gcgagcacgc acagcctcga tctttcgccg
     4261 gaggacgcgg cggcgcgcga cctgttggag aagacctacc gcgaggccgc gctcgaagcg
     4321 cccgcgctcg aagaggcgct cgccgccgcg gggccgaccc gcgagcgagc gcgcaagctg
     4381 ctgcaactgc tgctcgacgc gggcacgctc gtccgcgtcc gcgaagacct gctcttccac
     4441 cgcgacgcgc tcgccgcgct cacggtcgcg ctgcgcgact acggcgcgcg ccacgaaccc
     4501 gagcggctca tcgacgtggc cgccttcaag gaactctccg gcgtctcgcg caagtacgcc
     4561 atcccgctcc tcgaatactt cgaccgcgag cgcgtcacgc gccgcgcggg cgacaagagg
     4621 ctcatacttt agaacaggct tgaatcagcg cgggacgttg cccgctcgct cgttccgcgg
     4681 cgattcggac ggcgagcggt cggaagtctt cgacaagacg agcggcccgg acgtggagtc
     4741 gtgtccgggc cgctcgtttc gtgaagtccg tgtcggaggt cagaagccga agcggtcgcc
     4801 gtactcgatg gactggatga acgccttgac catctcggcc tcgacgtagt tgccgccgaa
     4861 ctggttgagc ttcaggagcc agaagtcgaa gccgtcgaag ttggtgtcct gcgggtcgtc
     4921 cgggttgcgc cgcagatacc cgaagtactg catcaggacg aacgccttgt tcgtctcggc
     4981 ctgccgcagc gtctcgtcct cggccaccgc gcgcaggact tgcgcccggg tcttcgtgcc
     5041 gttagtcaaa tcgctgacga gcgcgtcgcg ctcggcctgc gagagcgggt tgccggccct
     5101 cgtgtcgagg gcgtccacga acagcgccgc cgtcatcgac tgcgggtaga gcgtggagaa
     5161 ggcggtgcgt gcgacgaagg cgttgaagta ggccgccttg ttcgcttcga gctgctcggg
     5221 ccagcccggc gcgccgacga cgacgccgtc gccgatctgc cgcgtgtcga aaaggaagtc
     5281 gcgcaggcga atcggcacgg gcttgcccgc gaggtcgccg aaggcagcct tgtgcgtccg
     5341 gtaggccaga aagcccgtct cctggaattc gatggagagg aagaaggcgg cgctggtgtc
     5401 caccttcctg acttcgcggc actgaaggtt cgtgccgcac gactcgatgt tgtcaatcca
     5461 gaaggcgagg ccgcccgcgt cgggcacgcg gcctaaaaag tccgcgtagt gctggcggac
     5521 gaagaactcc gtgttgaagc tcgcgtcctt gacggggctg gggccgcccg ccgcgtcgtt
     5581 gtcgatgatg ttgacggtgg cgttggccgg cgcgccgagc gtggcacccg tcgggcctgt
     5641 cagcacgacg ttgaagctct cgccgtcgtc gtcgaagcgg tcgtcggtga tgacgatctg
     5701 gaagctcttg ctcgtctcgc cgggcgcgaa gcggaggcgc ccgagcgcgg cggtgtagtc
     5761 gcgtcggtcg ctggccgcgc cgtccgtggt ggcgaagttg acgaacgtct cgcccgacgt
     5821 gtcgcccgtg cgcgtgacgg tgacgttcac cgcgccgccg ccctcgtcgg cggtgaaggt
     5881 ttcggcgctg agctgcactt ggccggcgag gcggacggag aacacgtccg actccgtgtc
     5941 ggtgtcgcgc gggtcgaggt tgctggcctc gctcgtgaag acgacgac
//
LOCUS       DBNGOH010000014         5220 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2568243, whole genome shotgun sequence.
ACCESSION   DBNGOH010000014 DBNGOH010000000
VERSION     DBNGOH010000014.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5220)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5220)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5220
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2568243"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..229)
                     /locus_tag="AC159P_00280"
     CDS             complement(<1..229)
                     /locus_tag="AC159P_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HIR3693913.1"
                     /translation="MGNTADFLHPRDEIMRMIERIYRYRMTTTSGGNISLREEDGTIW
                     ITPARVDKGSIRREDIVRVLPDGSAEGLHKPS"
     gene            complement(371..1189)
                     /locus_tag="AC159P_00285"
     CDS             complement(371..1189)
                     /locus_tag="AC159P_00285"
                     /inference="COORDINATES: protein motif:HMM:NF019498.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR3693914.1"
                     /translation="MITRRQVLVAVVAACATFGVVVSAARTDKKPVMGSAIFEWEAVA
                     VKEQKTGARRQFFQSPTATLDELECHATTVNPGESPHAPHSHPEEEIIVVKEGTVEAL
                     AGGETRRAGPGSVIFQASNQLHGLRNVGTTQATYHVFRWKTPGAPRATSPLRSAVFDW
                     ESMKATTEKYGARRQLFQSRTATLDELESHVTTLNPGETTGPPHRHPEEALVIVKEGT
                     VEALTAGGEWRRVGPGSVVFEASNQLHGLRNAGETQAAYHVIKWHTPATPKPKK"
     gene            complement(1247..2245)
                     /gene="fbp"
                     /locus_tag="AC159P_00290"
     CDS             complement(1247..2245)
                     /gene="fbp"
                     /locus_tag="AC159P_00290"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HIR3693915.1"
                     /translation="MITTFQQHVLQEQARFPGASTEFSWLVSGISLATKMIQAQVRRA
                     GLTGILGTHGEVGEVNVQGEVQQKLDVYANEALIHCLSLRESIGIIASEENEQPLTVG
                     HESPNAKYAVVFDPLDGSSNIDVAVSVGTTFSVFRKPEGEGASDPARWVLQTGSKQVA
                     AGYVVYGSSTVLVYSTGRGVHCFTLDPSVGAYILTHENLRMPEQGAYYSINEAYCDRF
                     PDVYVRYLKRLRTGAAGRRYSSRYVGSMVADFHRTLLRGGVFVYPQTPDYPEGRLRLL
                     YEANPVAFIAEQAGGAATDGHQPILDIEPRDIHQRTPLVVGGRAEMAEFERCVREG"
     gene            complement(2242..3732)
                     /locus_tag="AC159P_00295"
     CDS             complement(2242..3732)
                     /locus_tag="AC159P_00295"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF012589.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HIR3693916.1"
                     /translation="MDDSLYIAVDLGAGSGRVFLAGVAPGELLLEEVRRFRYPPEESG
                     GHLRWDFPKIFAEIKTGLGAAGERARAMGRRVESIGVDSWGVDYGLIDEAGKLLENPI
                     CYRDERTRNAMEEVFAVVPREELFERTGIQLLVFNTIFQLHAHARAGVPERAARLLLI
                     PDLINFFLTGSAVTEYTNATTTQLVNARTGQWDGELLERLNLPANLLAEIVPAGAGVG
                     PLTAALADKLGLEGVRVVAPATHDTGSAIAGAPLEDGWAYISSGTWSLVGVEREEVLV
                     NSRVARHNFTNEGGAFGTVRFLKNVMGLWIFESCRKEWEARGLGVDYETLLNQAEALK
                     SSPGLIYPDDPRLFNPPSMLDAVAAQLSENGTERAPDDPLALTKVIMDSLALRYASVL
                     RTAEALTDEKLSGVRIVGGGSRNDYLNRATADAAGLAVVAGPVEATVTGNVLVQAVAA
                     GRFATLSEARRHVAENSNSKRFEPSGSTAWEERAERYAEIEARYAK"
     gene            complement(3819..5120)
                     /gene="rhaI"
                     /locus_tag="AC159P_00300"
     CDS             complement(3819..5120)
                     /gene="rhaI"
                     /locus_tag="AC159P_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003553857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose catabolism isomerase"
                     /protein_id="HIR3693917.1"
                     /translation="MATHFEIEDGFIAEQNRKAKEWVEEDYEHLAKILRRRNVDIEDL
                     TAKAGSFRVAVPSWGVGTGGTRFARFPGVGEPRGIYEKLEDCATINKLVRSTPAVSLH
                     IPWDKPENAAELNEFARARGLHFDAMNSNTFQDQVGQKHSYKFGSLTHPDAAVRRQAV
                     EHNVECIELGQQLGSKAHTVWIGDGGNFAGQQHFRRVLARYLESMRDIYKALPADWRL
                     FIEHKFYEPAFYSTVINDWGVSYHCARELGPQAYCLVDLGHHAPNVNIEMIVARLIQF
                     DKLAGFHFNDSKYGDDDLDSGSIKPFQLFLIFNELVDAELEGVEGFAPAYMLDQSHNV
                     TDPVESLMSSAVEVVRAYVQAHLVDREALNAAQENCDALMALATLKQAFTTDVSPILA
                     LARERAGGAIDPVAAYRASGYRQRKAEERPATKGAAASGIV"
BASE COUNT          799 a         1766 c         1750 g          905 t
ORIGIN      
        1 aagagggctt gtgcagcccc tccgccgagc cgtccggcag cacgcggacg atgtcctcgc
       61 ggcggatgct gcccttgtcc acgcgcgcgg gcgtgatcca gatggtgccg tcctcctcgc
      121 gcagcgagat gttgccgccc gaggtggtcg tcatgcggta acggtagatg cgctcgatca
      181 tccgcatgat ctcgtcgcgc gggtgcagga agtcggcggt gttgcccatc tttggctcgc
      241 aatcggttcg gacggaataa tatcagtaag cagtgagcgg tgagcagtgc gcagtagaaa
      301 gcttggaaga aattttgctc gccgcccgcc actcattgtc ttcgactgct caccgcttac
      361 cgctcactgc tcacttcttc ggcttcggcg tggcgggcgt gtgccacttg atgacgtgat
      421 acgcggcctg cgtctcgcct gcgttgcgca ggccgtgcag ttggttcgac gcctcgaaga
      481 cgaccgaccc cggcccgacg cggcgccact cgccgccggc tgtgagcgcc tcgaccgtgc
      541 cctccttgac gatgaccagc gcctcctccg ggtggcggtg cggcgggccg gtcgtctcgc
      601 cggggttgag cgtcgtgacg tgcgactcca gctcgtccag cgtcgccgtc cgcgactgga
      661 agagctgacg ccgcgcgccg tacttctcgg tcgtcgcctt catcgactcc cagtcgaaga
      721 ccgccgaccg taagggcgac gtggcgcgcg gcgcgccggg cgtcttccac ctgaagacgt
      781 ggtaggtcgc ctgcgtcgtc ccgacgttgc gcagcccgtg cagttggttc gacgcctgaa
      841 agatgaccga gccggggccg gcgcggcgcg tctcgccgcc cgccagcgcc tcgaccgtgc
      901 cctccttgac gacgatgatc tcctcctccg ggtgactgtg cggcgcgtgc ggcgattcgc
      961 cggggttgac ggtcgtggcg tggcactcca gctcgtccag cgtcgccgtc ggcgattgga
     1021 agaactggcg ccgcgcgccg gtcttctgct ctttcaccgc gacggcttcc cactcgaaga
     1081 tggcggaacc catgacgggc ttcttgtccg tgcgcgccgc cgacacgacg actccgaacg
     1141 tcgcgcacgc cgccacgacg gcgacgagca cctgccttcg cgttatcatc cgcgccctct
     1201 ctcctctcgc gtccgtcgct tcaaagacga atcacccgcg gcgtgttcaa ccttcgcgca
     1261 cgcagcgctc gaactcggcc atctcggcgc ggcccccgac gacgagcggc gtgcgctggt
     1321 ggatgtcgcg cggctcgatg tcgaggatgg gctggtggcc gtcggtggcc gcgccgcccg
     1381 cctgctcggc gatgaaggcg acggggttcg cttcgtacaa aagtcgcagg cggccttcgg
     1441 ggtagtcggg cgtctgcgga tagacgaaga cgccgccgcg cagcagcgtc cggtgaaagt
     1501 ccgcgaccat cgagccgacg tagcgcgaag agtagcggcg ccccgccgcg cccgtacgca
     1561 gccgcttgag gtagcgcacg tagacgtcgg ggaagcggtc gcagtaagcc tcgttgatcg
     1621 agtagtaggc gccctgctcc ggcatccgca ggttctcgtg cgtcaggatg taggcgccga
     1681 ccgaagggtc gagcgtgaag cagtggacgc cgcggcccgt gctgtagacg agcaccgtcg
     1741 aggagccgta gacgacgtac ccggcggcga cctgcttgct gccggtctgc aagacccacc
     1801 gcgcggggtc gctcgcgccc tcgccctcgg gcttgcggaa gacggagaag gtggtgccga
     1861 ccgagacggc gacgtcgatg ttcgaggagc cgtccagagg gtcgaagacg acggcgtact
     1921 tcgcgttcgg cgactcgtgc ccgacggtga gcggctgctc gttctcctcc gaggcgatga
     1981 tgccgatgct ctcgcgcagg gagaggcaat ggatgagggc ttcgttggcg tacacgtcga
     2041 gcttctgctg cacctcgccc tgcacgttca cctcgccgac ctcgccgtgc gtgccgagga
     2101 tgccggtgag gcccgcgcgc cgcacctgcg cctgaatcat cttcgtcgcc agagagatgc
     2161 ccgagacgag ccacgagaac tccgtcgagg cgccggggaa ccgcgcctgc tcctgtagga
     2221 cgtgctgctg gaaggtcgta atcatttcgc gtaacgcgcc tcgatctccg cgtagcgctc
     2281 cgcgcgctcc tcccacgcgg tcgagccgga aggctcgaag cgcttcgagt tcgagttctc
     2341 ggcgacgtgc cgccgcgcct ccgagagcgt ggcgaagcgc ccggcggcga ccgcctgcac
     2401 gagcacgttg cccgtgacgg tcgcctcgac cggcccggcc accaccgcca gcccggcggc
     2461 gtcggcggtc gcgcggttca ggtaatcgtt gcggctgccg ccgccgacga tgcgcacgcc
     2521 ggagagcttt tcgtccgtca aagcctccgc ggtgcgcagc accgaggcgt agcgcagcgc
     2581 cagcgaatcc atgatgacct tcgtcagcgc gagcgggtcg tcgggcgcgc gctccgtacc
     2641 gttctccgag agctgcgcgg ccaccgcgtc gagcatgctc ggcgggttga acagacgcgg
     2701 gtcgtccggg tagatgaggc cgggcgagct tttcagcgcc tcggcctgat tgagcagcgt
     2761 ctcgtaatcg acgccgaggc cgcgcgcctc ccactccttc cggcacgact cgaagatcca
     2821 cagccccatc acgttcttca aaaaacgcac ggtgccgaac gcgccgccct cgttcgtgaa
     2881 gttgtggcgc gcgacacgag agttcacgag cacctcttcg cgctcgacgc cgaccaaaga
     2941 ccacgtgccc gaagagatgt aggcccagcc gtcttcgagc ggcgcaccag cgatggcgct
     3001 gcccgtgtcg tgcgtcgcgg gcgcgacgac gcgcacgcct tcgaggccga gcttgtccgc
     3061 gagcgccgcc gtcagcggcc ctacaccagc acccgccggc acgatctccg ccagcaggtt
     3121 cgctggaagg ttcaggcgct ccaacaactc gccgtcccac tgccccgtcc gcgcgttgac
     3181 gagctgcgtc gtcgtcgcgt tcgtgtactc tgtgacggcg ctgcccgtca ggaaaaaatt
     3241 tatcaggtcg gggatgagca gcagccgcgc cgcccgttcg gggacaccag cgcgcgcgtg
     3301 cgcgtggagc tgaaagatgg tgttgaagac gaggagctgg atgcccgtcc gctcgaacag
     3361 ctcctcgcgc ggcacgacgg cgaagacctc ttccatcgcg ttccgcgtcc gctcgtcgcg
     3421 gtagcagatc gggttttcga ggagcttgcc cgcctcgtcg atcaaaccgt aatcgacgcc
     3481 ccagctatcg acgccgatgc tctccacgcg ccgccccatc gcccgcgcac gctcgcccgc
     3541 cgcgccgagg cccgtcttga tctccgcgaa aattttcggg aagtcccaac gcaggtgccc
     3601 gcccgactct tccggcgggt aacggaaccg ccgcacctct tcgagcagca actcgcccgg
     3661 cgccacgccc gccagaaaga cgcgcccact gcccgcgccg aggtcaaccg cgatgtagag
     3721 cgaatcgtcc atgcagaggt cagaggtcag aggtcagagg tcagtgaaag acagtttgtt
     3781 ttaactgacc tctgacctct gacctctgac ctctgaactt agactattcc cgaggccgcc
     3841 gcgcccttcg tggcggggcg ctcttcggcc ttgcgctggc ggtagccgct ggcgcggtag
     3901 gcggcgacgg ggtcgatggc gccgcccgcg cgttcgcgcg cgagggcgag gatgggggac
     3961 acgtccgtcg tgaaggcctg cttgagggtc gccagcgcca tcagcgcgtc gcagttctcc
     4021 tgcgcggcgt tgagcgcctc gcggtcaacg aggtgcgcct gcacgtaggc ccggacgact
     4081 tcgacggcgc tcgacatcag ggactccacg gggtcggtca cgttgtgcga ctggtcgagc
     4141 atataggcgg gcgcgaagcc ctcgacgcct tccagctcgg cgtccaccag ttcgttgaag
     4201 atgaggaaga gctggaaggg cttgatcgag ccggagtcga ggtcgtcgtc gccgtacttc
     4261 gagtcgttga agtggaagcc cgccagcttg tcgaactgga tgaggcgcgc gacgatcatc
     4321 tcgatgttga cgttcggcgc gtggtggccg agatcgacga ggcagtaagc ctgcggcccc
     4381 agctcgcgcg cgcagtggta gctgacgccc cagtcgttga tgacggtcga gtagaacgca
     4441 ggctcgtaga acttgtgctc gatgaagagc cgccagtccg caggcagcgc cttgtagatg
     4501 tcgcgcatgc tttcgaggta gcgcgcgagc acgcggcgga agtgctgttg gccggcgaag
     4561 ttcccgccgt cgccgatcca gaccgtgtgt gccttcgagc ctagttgctg gccaagctct
     4621 atgcattcga cgttgtgctc gacggcctgg cggcggacgg cggcatcggg gtgcgtcagc
     4681 gacccgaact tgtaggagtg cttctggccg acctgatcct gaaacgtgtt ggagttcatc
     4741 gcgtcgaagt gcaggccgcg cgcgcgcgcg aactcgttca gctccgcggc gttctccggc
     4801 ttgtcccagg ggatgtgcag cgagacggcg ggcgtcgagc ggacgagctt gttgatggtc
     4861 gcgcagtctt cgagcttctc gtagatgccg cgcggctcgc ccacgcccgg aaagcgcgcg
     4921 aagcgcgtcc cgcccgtgcc gacgccccac gaaggcaccg ccacgcggaa gctgccggcc
     4981 ttcgccgtca ggtcttcgat gtccacgttg cgccgccgca ggattttggc gaggtgctcg
     5041 tagtcctctt cgacccactc tttcgccttc cggttctgct cggcgatgaa cccgtcctcg
     5101 atctcgaaat gcgttgccat ccgactcacc tccgcaaagt gttattgcct ccgcacggcc
     5161 acgaaaagct caaggttcag tggaacactc gtcgtaaacg gaaaccgtgg gggcggggct
//
LOCUS       DBNGOH010000015         3804 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1468481, whole genome shotgun sequence.
ACCESSION   DBNGOH010000015 DBNGOH010000000
VERSION     DBNGOH010000015.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3804)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3804)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3804
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1468481"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(96..401)
                     /locus_tag="AC159P_00305"
     CDS             complement(96..401)
                     /locus_tag="AC159P_00305"
                     /inference="COORDINATES: protein motif:HMM:NF024183.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double zinc ribbon domain-containing protein"
                     /protein_id="HIR3693918.1"
                     /translation="MSYEERQPPTAGGPPTAPHRYSRACPFCGAPAADRFCGACGRDT
                     TAPRRPCTRCKRMVPSSERVCWNCGAGFTSDMWWKIPLIVFLFLLAFIVSIVIALLS"
     gene            complement(417..851)
                     /locus_tag="AC159P_00310"
     CDS             complement(417..851)
                     /locus_tag="AC159P_00310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3693919.1"
                     /translation="MARFKDPNFVAAAFQPYLEPGEQLKHWAFGVKQPSMLLILPLFL
                     LAILPGAIAVALLTKEYIVGLTDRRFIVLRFKGSKINVQEVLEYPLANLPPVKASTGG
                     LFTHIKIEDPAKPFVAKFHRMGMPGNREHGQAIEAALTGKAA"
     gene            complement(930..1949)
                     /locus_tag="AC159P_00315"
     CDS             complement(930..1949)
                     /locus_tag="AC159P_00315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3693920.1"
                     /translation="MSGRRKRKAKGKQKQSRHKNRARRARPAAPREAAGPRPATRRGV
                     KLMADRKRPSDQRSTEDILREMEELSKSIDSTISGISADQSAPSAQAAPSSSASRRGG
                     GAMKSLLNFFVSVEPEEGAPADAEAPPPDASDVPAEATPPARAAGPRVADLVAGEAPP
                     TFEPPDAGAGGDLAAKSLEEIYTEAGLGDSQCSADELAKLLENPAIASQPMSVKVVAV
                     NLALSAKGVGTDVPIADAVRRDRALDAYQAMLDERARAAEERNAEQIQRLTQEAEEYL
                     KRKQVEMEALRAETAEAKRQSFDFAARREAEEKRLAELISPFLEGKPSPVTVGNQGAE
                     KKTGP"
     gene            complement(1984..2910)
                     /locus_tag="AC159P_00320"
     CDS             complement(1984..2910)
                     /locus_tag="AC159P_00320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3693921.1"
                     /translation="MPDERDDKFGPINFVKEVLKSEWNLGFLGVMAFLMLVVNFWAFL
                     PLLVAGQLGAALLAQMPIVQRYIKMKKVWQKKESDEEAEKRIVLALPSNHQADFHTVR
                     ALCEEIERRSSELGTEGAGRMLADVVAKLSSFRYEYARMLRARHLLSTRNYRQLQNTL
                     TQQIEQGEGALAREEARQVRYALTQNLNILTQRLARIKRLDELVRLLDARLQVIKNSL
                     GLIQDEVYTFTDVAGIQGLVDNLLINLSISDEFRAEYEDVLNVEAEGPALSALEGVAA
                     SSSAAASASSANTPEAAAERARRAQRQSQRIK"
     gene            complement(3013..3402)
                     /locus_tag="AC159P_00325"
     CDS             complement(3013..3402)
                     /locus_tag="AC159P_00325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3693922.1"
                     /translation="MGQIAGQVHGKFKVFAGPLHTDNTIGPLAEEISSFVRESHAAAK
                     SIGVEYLESADRLVITLGYRDDEEGYPVKLSSVSLGKIEAVGSDFAELERRMSEASAQ
                     FTNIICHELYITEDHDFMMIVMTHVEQ"
     gene            complement(3481..>3804)
                     /locus_tag="AC159P_00330"
     CDS             complement(3481..>3804)
                     /locus_tag="AC159P_00330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3693923.1"
                     /translation="ILDEPFSGLDPVNITTVIELIARTANQHSRNTFVIVTHDVTSAL
                     IVSDTVYLLGRERDEKGNPIPGARVMKQYDLIAEGLAYQPQIEDLPRFSEIRKEIKLV
                     EFPKL"
BASE COUNT          567 a         1192 c         1323 g          722 t
ORIGIN      
        1 cggctggggg gcaggggagc ggcgcgagag gcggaagtga gagggcgttc agccttctcg
       61 cttccgcctt catgctttgt tcggcgtcgg cgcgactatg agagcagcgc gatcacgatg
      121 ctgactatga aggcgagcag gaagaggaag acgatgagcg ggattttcca ccacatgtcg
      181 ctggtgaagc ccgcgccgca gttccagcag acgcgctcgc tgctcggcac catccgcttg
      241 caacgtgtgc aggggcggcg cggcgcggtc gtgtcgcgcc cgcacgcgcc gcagaaacgg
      301 tcggcggcgg gcgccccgca gaaagggcac gcgcgcgaat atcgatgcgg ggcggtagga
      361 gggccgcccg ccgtcggcgg ttgtctctct tcgtaactca tcgcggcctc ccctcgtcag
      421 gccgccttgc cggtgagggc cgcctctatc gcctgcccgt gctcgcggtt gccgggcatg
      481 cccatgcggt ggaacttggc gacgaacggc ttggccgggt cttcgatctt gatgtgcgtg
      541 aagagtccgc cggtcgaagc cttcaccggc gggaggttgg cgagcgggta ctccaacacc
      601 tcctgcacgt tgatcttgct gcccttgaag cggagcacga tgaagcgccg gtcggtcagc
      661 ccgacgatgt actccttcgt cagcagcgcg acggcgatgg cgccgggcag gatggcgagg
      721 aggaagagcg ggaggatgag gagcatgctc ggctgcttga cgccgaaggc ccagtgcttc
      781 agttgttctc ccggttcgag gtaaggctgg aaagccgcgg cgacgaagtt gggatctttg
      841 aatcttgcca tctctctcct ctggggttga agggaaaaca actcgcgcga ggtggcttcg
      901 gacgactccg gcggaagatg aaaaccgact tacggcccgg tctttttctc ggcgccctga
      961 ttgccgacgg tgacggggct gggcttgcct tcgaggaagg ggctgatgag ttcggcgagc
     1021 cgcttctctt cggcctcgcg gcgcgcggcg aagtcgaagg actggcgctt ggcctcggcg
     1081 gtctcggcgc gcagcgcctc catctcgacc tgcttgcgct tgaggtactc ttcggcctct
     1141 tgcgtgagcc gctgaatctg ctcggcgttg cgctcttcgg cagcgcgcgc gcgctcgtcg
     1201 agcatggcct gatacgcgtc gagcgcgcgg tcgcggcgca cggcgtcggc gatgggcacg
     1261 tccgtcccga cgcctttcgc cgacagagcg aggttgacgg cgacgacctt gacgctcatc
     1321 ggctggctcg cgatggcggg attctcaagc agcttcgcca actcgtccgc gctgcactgc
     1381 gaatcgccga gtccggcctc cgtgtaaatc tcttcgagcg acttggcggc gaggtcgccg
     1441 cccgcgcccg cgtccggcgg ctcgaaggtc ggcggcgctt cgcccgcgac gaggtctgcg
     1501 acgcgcggcc ccgcggctcg cgcgggcggc gtcgcttcgg cgggcacgtc cgaagcgtcg
     1561 gggggcggcg cctcggcgtc ggcgggcgcg ccctcttcgg gttcgaccga gacgaagaag
     1621 ttgagcagcg acttcatcgc gccgccgccg cgcctcgacg cggacgaaga gggcgcggcc
     1681 tgcgcgctcg gcgcgctctg atccgcggag atgccggaga tggtcgagtc gatgctcttc
     1741 gacagctcct ccatctcgcg caggatgtct tcggtgctcc gctggtcgct cggtcgcttg
     1801 cggtcggcca tcagtttcac tcctcgtcgt gtcgcgggcc gaggcccggc ggcttcgcgc
     1861 ggggcggcgg ggcgtgcgcg ccgcgcgcgg ttcttgtgtc gtgactgctt ctgcttgccc
     1921 ttcgccttgc gctttcttct gccgctcatc acgcgccctc cctcgcggtc gggacggctg
     1981 ccgttacttg atgcgctgcg actgccgctg cgcgcgccgc gcccgctcgg cggcggcctc
     2041 cggcgtgttc gccgaggaag cggaggcggc ggcggaagat gacgcggcca cgccttcgag
     2101 cgcggagagc gccgggcctt ccgcctcgac gttcaaaacg tcctcgtact cggcacggaa
     2161 ctcgtcgctg atgctgaggt tgatgagcag gttatcgacc agcccctgga tgcccgccac
     2221 gtccgtgaag gtgtagacct cgtcctggat gaggccgagc gagttcttga tgacttgcag
     2281 ccgcgcgtcc aagaggcgga cgagttcgtc gagccgcttg atgcgcgcga ggcgctgcgt
     2341 gaggatgttg aggttctgcg tcagcgcgta gcgcacctgc cgcgcctcct cgcgcgcgag
     2401 cgcgccctcg ccctgttcga tctgctgcgt gagcgtgttc tgcaactggc ggtagttgcg
     2461 cgtcgaaagc aggtggcgcg cgcgcagcat ccgcgcgtac tcgtagcgga aggaggagag
     2521 cttcgccacc acgtccgcca gcatcctccc ggcgccctcc gtgccgagct ccgacgagcg
     2581 ccgctcgatc tcctcgcaga gcgcgcgcac cgtgtggaag tcggcctgat ggttcgaggg
     2641 gagcgccagc acgatgcgct tctcggcctc ctcgtccgac tccttcttct gccacacctt
     2701 cttcatcttg atgtagcgct ggacgatggg catctgcgcc agcagcgccg cgcccagctg
     2761 cccggccacg agcagcggca ggaaggccca gaagttgacg accagcatga ggaacgccat
     2821 cacgccgagg aagcccagat tccactcgct cttcaggact tccttgacga agttgatggg
     2881 gccgaatttg tcgtcgcgct cgtcaggcat aacaggaaac gatgaatgcg gaatgcggaa
     2941 tgacgaatga aagacggctt gatcgatgac aggcaccgtg cttcttattc atcattccgc
     3001 attccgcatt catcattgtt ctacgtgcgt catgacgatc atcatgaagt cgtggtcttc
     3061 ggtgatgtag agttcgtggc agatgatgtt ggtgaactgc gcgctggcct cggacatgcg
     3121 gcgttcgagt tcggcgaagt cggagcctac ggcttcgatc ttgccgagcg agaccgagga
     3181 gagtttgacg gggtagccct cctcgtcgtc gcggtagccg agggtgatga cgaggcggtc
     3241 ggcgctctca aggtactcga cgccgatgga cttggcggcg gcgtgcgact cgcggacgaa
     3301 gctgctgatc tcttcggcga gcgggccgat ggtgttgtcc gtgtggagcg gcccggcgaa
     3361 gaccttgaac ttgccgtgca cctgtccggc gatctgtccc ataaacttct ccttagcggt
     3421 caaaaaagtt gtgacggcga ggcccgccgg ccctctcgaa tccggagcca ggtaacttta
     3481 tcaaagcttg gggaactcga ccaacttgat ctccttgcga atctcggaga agcgcggcag
     3541 gtcttcgatc tgcggctggt aggccagccc ctccgcgatc aggtcgtact gcttcatcac
     3601 gcgcgcgccc ggaatcgggt tccccttctc gtcgcgctcg cgccccagca gatagaccgt
     3661 gtccgagacg atgagcgccg aggtcacgtc gtgcgtgacg atgacgaagg tgttgcgcga
     3721 atgctggttg gcggtgcgcg cgatgagttc gatgacggtc gtgatgttga cggggtcgag
     3781 gccggagaac ggctcgtcga ggat
//
LOCUS       DBNGOH010000016         4392 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3668267, whole genome shotgun sequence.
ACCESSION   DBNGOH010000016 DBNGOH010000000
VERSION     DBNGOH010000016.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4392)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4392)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4392
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3668267"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..329)
                     /locus_tag="AC159P_00335"
     CDS             complement(<1..329)
                     /locus_tag="AC159P_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3341 domain-containing protein"
                     /protein_id="HIR3693924.1"
                     /translation="MALKKKTRPATYGLMAEFDNPTDVVNAAHSVHEAGYRRIDAYSP
                     YPIEALSEAIGVHSTKLPIIVLIGGIIGGLAGYFLQYFTHVVDYPLIVGGKPFHSWPA
                     FIPVTFE"
     gene            complement(472..1863)
                     /gene="nrfD"
                     /locus_tag="AC159P_00340"
     CDS             complement(472..1863)
                     /gene="nrfD"
                     /locus_tag="AC159P_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020713378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HIR3693925.1"
                     /translation="MSRIDNPHDDVRIHTTPAVIEPGHTFGTVTDKISSIVLRKRTPL
                     AWYIAIAVAFLITQVMAGTIFLLVTTGIGIWGNNQPVGWGFDIINFVWWIGIGHAGTL
                     ISAILLLLRQEWRTSINRFAEAMTLFAVACAGLFPLLHTGRPWLAYWMFPIPNTMAMW
                     PQFRSPLMWDVFAVSTYATISALFWYTGLIPDLATLRDRARSKFGRVVYGMAALGWRG
                     SARHWARYEATYLLLAGLSTPLVLSVHTIVSFDFAVGIIPGWHATIFPPYFVAGAIYA
                     GFAMVLTLVIPLRKFYGLEDFITDRHLRNSAKVMLATGLIVAYGYMMETFMAWYSANT
                     YEEFMIRNRFFGPYAFYYWALIVCNVIVPQILWVRPFRHSTAALFVVAMFVNVGMWLE
                     RFVIIPISLHRDFLPSSWGFYSPTIFDFSMFFGTIGFFFFLMLLFLRFLPVISIFEMR
                     TMVPQAEIKEEKG"
     gene            complement(1874..>4392)
                     /locus_tag="AC159P_00345"
     CDS             complement(1874..>4392)
                     /locus_tag="AC159P_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR3693926.1"
                     /translation="RRAREGQPREMNRLYAIETTPTNTGLVADHRLAVRPSELEQLAR
                     AVAAGVGAGGVAAPSGLKPEQTNFVNAVAADLKQHAGRSVVIAGDEQPPVVHALAHAM
                     NAALTNVGKTVSYFDPVEELPADQLEDLRALLGEVDADTVDMLVIVGGNPVFNAPADL
                     DFGNRIRARSGDRERVPLRVHLSLYEDETSELCHWHIPQAHFLEAWSDTRAYDGTVSV
                     IQPLITPLYNGKSAHEFLAAFSDTPERSGYEIVRDYWSSPNAGSRVQIAGRAGAASQP
                     TPTASPAASQPTPAATASPAPTPTPRPAASPAPASSPSTTAAAAPAASPSADFEKNWR
                     KWVHDGVIPGQQRPAKTLGLRGDFAAAAPAAAPVGGNQLEIVFRPDPSVYDGRFANNG
                     WLQELPKPLTKLTWDNAAIISPATAERLGTGVAPDGAVEGGPVRVNRLSKKGGEIIAD
                     VVKLTHKGREVEAPLFLMPGQPDNVITVHLGYGRRRAGRVGGNAYEKHVVGFDAYRLR
                     TSDALWQGADVRAELTGDTYSLASTQIHFQMEGREVVRHGSFEQYRHDPTLGRAGEHA
                     APAPPVGENKPPHDNESLYTEHHYDDPAFNSRLYRWGMAIDLSTCVGCNACIIACQAE
                     NNIPVVGKEQVLRSREMHWLRVDAYFRRAADNPAGVYFMPVPCMHCENAPCEPVCPVH
                     ATVHSAEGTNDMVYNRCIGTRYCSNNCPYKVRRFNFLLYQDWDTPSLKMMRNPEVTVR
                     SRGVMEKCTYCIQRIEYAKIESEKENRRVRDGEIVTACQGACPTEAIVFGDLSDHDSK
                     VAKMHAQKHRYDLLAELNTRPRTVYLSAVRNPNPALGGEA"
BASE COUNT          703 a         1339 c         1634 g          716 t
ORIGIN      
        1 atctcgaagg tgaccgggat gaaggcgggc caactgtgga agggcttgcc gccgacaatc
       61 aaagggtaat cgacgacgtg tgtgaagtac tgcaagaaat agccggccag cccgccgatg
      121 atgccgccga ttaaaacgat gatcggcagc ttcgtcgagt ggacgccgat ggcctccgag
      181 agcgcctcga tggggtaggg cgagtaggcg tcgatgcgcc ggtagcccgc ctcgtggacg
      241 ctgtgcgcgg cgttcaccac gtcggtcggg ttgtcgaact cggccatcag cccgtaggtc
      301 gccggcctcg tttttttctt tagcgccacg ccagccctcc tttcgtcggg acggggagac
      361 aaggcgacgg ggcgacgggg cgataaaaaa gcctcgccgg cacgcccgcc cgtcgcgccg
      421 cgtgcgcttc acgcaactct ctccccgtca ccccgtcacc ccttctcccc ttcatccctt
      481 ctcctccttg atctcggcct gcggcaccat cgtccgcatc tcgaagatgg agatgacggg
      541 gaggaaccgg aggaagagga gcatcaggaa gaagaagaag ccgatggtgc cgaagaacat
      601 cgagaagtcg aagatggtcg gcgagtagaa gccccacgaa gagggcagga agtcgcggtg
      661 gaggctgatc gggatgatga cgaagcgctc cagccacatg ccgacgttga cgaacatggc
      721 gacgacgaag agcgccgcgg tgctgtggcg gaacgggcgc acccaaagaa tctgcgggac
      781 gatgacgttg cagacgatga gcgcccagta gtagaacgcg tacgggccga agaagcggtt
      841 gcggatcatg aactcttcgt aggtgttggc gctgtaccac gccatgaagg tctccatcat
      901 gtacccgtag gcgacgatga ggccggtggc gagcatcacc ttcgccgagt tccgcaggtg
      961 ccggtcggtg atgaagtctt cgaggccgta gaacttcctg agcgggatga cgagcgtcag
     1021 caccatcgcg aagcccgcgt agatggcgcc cgcgacgaag tagggcggga agatcgtcgc
     1081 gtgccagccg gggatgatgc cgaccgcgaa gtcgaagctg acgatggtgt gcacggagag
     1141 gacgagcggc gtcgagagtc cggcgagcag caggtaggtc gcctcgtagc gcgcccagtg
     1201 gcgcgcgctg ccgcgccagc ccagagcggc catgccgtag acgacgcgcc cgaacttcga
     1261 gcgcgcgcgg tcgcgcagcg tcgccaaatc cgggatcagc cccgtgtacc agaagagcgc
     1321 cgagatggtg gcgtaggtcg agacggcgaa cacgtcccac atcaaagggc tgcggaactg
     1381 cggccacatg gccatcgtgt tcgggatcgg gaacatccag taggcgagcc acggcctccc
     1441 ggtgtgcagc agcgggaaga ggcccgcgca ggccacggcg aagagcgtca tcgcctcggc
     1501 gaagcggttg atcgaggtgc gccactcctg ccgcagcagc agcaggatgg ccgagatcag
     1561 ggtgcccgcg tggccgatgc cgatccacca gacgaagttg atgatgtcga agccccagcc
     1621 gacgggctgg ttgttgcccc agatgccgat gccggtcgtg acgagcagga agatggtgcc
     1681 ggccatgacc tgcgtgatga ggaaggcgac ggcgatggcg atgtaccacg cgaggggcgt
     1741 gcgcttgcgc aggacgatgg agctgatctt gtccgtgacg gtgccgaagg tgtgccccgg
     1801 ctcgatcacg gcgggcgtgg tgtggatgcg tacgtcgtcg tgcgggttat cgatcctcga
     1861 catgtgacta cctttacgcc tcccctccga gcgccgggtt cgggttacgc accgccgaca
     1921 ggtagacggt gcgcggcctc gtgttcaatt ccgcgagcag gtcgtagcgg tgcttctgcg
     1981 cgtgcatctt cgccaccttg ctgtcgtggt cggagaggtc gccgaagacg atggcctcgg
     2041 tcgggcacgc gccctgacag gccgtgacga tctcgccgtc gcggacgcgg cggttctcct
     2101 tctcggactc gatcttcgcg tactcgatgc gctggatgca gtaggtgcac ttctccatca
     2161 cgccgcgcga gcgcaccgtc acctccgggt tgcgcatcat cttcaaagaa ggcgtgtccc
     2221 agtcctgata gagcaggaag ttgaagcggc gcaccttgta ggggcagttg ttcgagcagt
     2281 agcgcgtgcc gatgcagcgg ttgtagacca tgtcattcgt gccctcggcg ctgtggacgg
     2341 tggcgtggac ggggcacacc ggctcgcagg gcgcgttctc gcagtgcatg cagggcacgg
     2401 gcatgaagta gacgcccgcc gggttgtcgg cggcgcggcg gaagtaggcg tccacgcgca
     2461 gccagtgcat ctcgcgcgag cgcaagacct gctccttgcc gaccacgggg atgttgttct
     2521 cggcctggca ggcgatgatg caggcgttgc agccgacgca ggtcgaaaga tcgatggcca
     2581 tgccccagcg gtagaggcgc gagttgaagg cggggtcgtc gtagtggtgc tcggtgtaga
     2641 gcgactcgtt gtcgtgcggc ggcttgttct cgccgacggg cggggcgggc gccgcgtgct
     2701 cgccggcgcg cccgagcgtc gggtcgtgcc ggtactgctc gaacgagccg tggcggacga
     2761 cctcgcgccc ctccatctgg aagtgtatct gcgtcgaggc gagcgagtag gtgtcgcccg
     2821 tcagctcggc cctcacgtcc gccccctgcc agagcgcgtc agaggtgcgc aggcggtaag
     2881 cgtcgaagcc gacgacgtgc ttctcgtagg cgttgccgcc gacgcggccc gcgcgccgcc
     2941 gaccgtagcc gaggtggacg gtgatgacgt tgtcgggctg gcccggcatg aggaagaggg
     3001 gggcttcgac ctcgcgcccc ttgtgcgtga gcttgacgac atcggcgatg atctcgccgc
     3061 ccttcttcga gagccggttg acgcgcaccg gcccgccctc gaccgcgccg tcgggcgcga
     3121 cgccggtgcc gagccgctcg gcggtggcgg ggctgatgat ggcggcgttg tcccacgtca
     3181 gcttcgtcag gggcttcggc agctcttgca gccagccgtt gttggcgaag cgaccgtcgt
     3241 agacggaagg gtctgggcgg aagacgattt cgagctggtt gccgccgacc ggcgccgccg
     3301 cgggagcagc cgccgcgaaa tcgcccctca agccgagggt cttcgccggg cgctgctggc
     3361 cggggatgac gccgtcgtga acccacttgc gccagttctt ctcgaagtcc gccgaagggc
     3421 tggccgcggg agcagccgcc gccgtcgtcg aaggcgaaga ggcgggcgcg gggctggccg
     3481 ccggtcgcgg tgtcggagtc ggcgcggggc tggcggtcgc agccggtgtg ggctgcgagg
     3541 cggcgggcga cgcggtcggc gtcggctgcg aggcggcgcc cgcgcgcccc gcgatctgca
     3601 cgcggctccc ggcgttcggc gaagaccagt agtcgcggac gatctcgtag cccgaacgct
     3661 cgggcgtgtc ggagaaggcg gcgaggaatt cgtgcgcgct cttgccgttg tagagcggcg
     3721 tgatgagcgg ctggatgacg gagaccgtgc cgtcataggc gcgcgtgtcc gaccacgctt
     3781 cgaggaagtg cgcctgcggg atgtgccagt ggcacagctc cgaggtctcg tcttcgtaga
     3841 ggctgaggtg gacgcgcagt gggacgcgct cgcggtcgcc gcttctggcg cggatgcggt
     3901 tgccgaagtc gaggtcggcg ggcgcgttga agacgggatt gccgccgacg atgacgagca
     3961 tgtccaccgt gtccgcgtcc acttcgccga ggagcgcgcg caggtcttcg agctggtcgg
     4021 cgggcagctc ttcgaccggg tcgaagtagg agacggtctt gccgacgttg gtgagcgcgg
     4081 cgttcatcgc gtgcgcgagc gcgtgaacca cgggcggctg ctcgtcgccc gcgatgacga
     4141 cggagcgacc ggcgtgctgc ttgaggtctg cggcgacggc attgacgaag ttcgtctgct
     4201 cgggcttgag accggacggg gcggcgactc cgcccgcgcc gacccctgcg gcgacggcgc
     4261 gcgcgagttg ttccagctcg ctcgggcgca ccgcgagccg gtggtcggcg acgaggccgg
     4321 tgttggtcgg cgtcgtctcg atggcgtaga ggcggttcat ctcgcgcggc tggccttcgc
     4381 gggcgcggcg gc
//
LOCUS       DBNGOH010000017         1708 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1102544, whole genome shotgun sequence.
ACCESSION   DBNGOH010000017 DBNGOH010000000
VERSION     DBNGOH010000017.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1708)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1708)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1708
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1102544"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..603
                     /gene="nth"
                     /locus_tag="AC159P_00350"
     CDS             <1..603
                     /gene="nth"
                     /locus_tag="AC159P_00350"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007904991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HIR3693927.1"
                     /translation="KKEHPDARCSLDHNGPLELLVATILSAQCTDERVNLVTADLFRK
                     YRSCEDYANAPAAELEQDIRSTGFFRNKAKSIQGACRVLLADYGGRVPERMEDLLKLP
                     GVARKTANVVLGNAYGIASGVVVDTHVSRLSARLALSAHTAPEKIERDLMQLVPESDW
                     VLFSHLLIAHGRQVCKARAPLCAECLLEPFCPSSLLKAAQ"
     gene            complement(794..>1708)
                     /locus_tag="AC159P_00355"
     CDS             complement(794..>1708)
                     /locus_tag="AC159P_00355"
                     /EC_number="3.2.2.-"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase family protein"
                     /protein_id="HIR3693928.1"
                     /translation="YPAGGLKDSMDFRIDAPRGFDFKRTLGSHGWSELAPCEWVNEGE
                     LRRVLDLGEREPVTLTITGDRSGVNVRAARRLGKREAAQVLRDVRHMFRLDDDMTEFY
                     GALSAEPGFAWVERDGAGRLLRSPTVYEDLVKSIMTTNCSWSLTRKMVTGLVNGLGRA
                     SKAGGRTFPTAEVMAAQPLDFYRDVVRAGYRAPYLAELAGRVAAGELDVEGWAASELP
                     TAELKREMKRVKGVGDYAAENLLKLVGRYDVLALDSWVRAKFAKVRNKGRACDDKKIA
                     RFYSRYGHWRGLALWCDMTRDWFEESRQ"
BASE COUNT          290 a          626 c          514 g          278 t
ORIGIN      
        1 aagaaagagc acccggacgc gcgttgcagc ctcgaccaca acggcccgct cgaactcctc
       61 gtcgccacca tcctctccgc gcagtgcact gacgagcgcg tgaacctcgt caccgccgac
      121 ctgttccgca agtaccggag ctgcgaagac tacgcgaacg cgcccgccgc cgagcttgag
      181 caagacatcc gctcgaccgg cttcttccgc aacaaggcga agtcgattca gggcgcctgc
      241 cgcgtgctgc tcgcggacta cggcgggcgc gtgcccgagc ggatggagga cttgctgaaa
      301 ctccccggcg tcgcgcgcaa gacggcgaac gtcgtcctcg gcaacgccta cggcatcgcc
      361 tccggcgtcg tcgtcgatac gcacgtctcg cgcctctccg cgcgcctcgc gctctcggcc
      421 cacaccgccc ccgagaagat cgagcgcgac ctcatgcagc tcgtccccga aagcgactgg
      481 gtgctcttct cgcacctcct catcgcccac ggtcgccaag tctgcaaggc gcgcgccccg
      541 ctctgcgccg agtgcctcct cgaaccgttc tgcccctctt cgctgctcaa ggctgcgcag
      601 taggcagtag gcggtaggca gttaaaacag aatacagaaa tcagaagtcg gaagtcggaa
      661 gtcggacaga caaagaggct tgggaggtaa agtgccaagc ctctgcgtgc gacaggcagt
      721 aaagtgaaga ggcttgagat gaaatgtctc aagcctcttc cttcttctgt cttgactgcc
      781 tactgccaac cgcctactgc ctactctcct cgaaccagtc gcgcgtcatg tcgcaccaga
      841 gggcgaggcc gcgccagtgg ccgtagcgcg agtagaagcg cgcgatcttc ttgtcgtcgc
      901 aggcgcgccc cttgttgcgc accttcgcga acttcgcgcg cacccatgag tcgagcgcca
      961 gcacgtcgta gcgcccgacg agcttcaaca ggttctccgc cgcgtagtcg ccgacgccct
     1021 tcacacgctt catctcgcgc ttcagctcgg cggtcggcag ctcgctcgcg gcccagccct
     1081 cgacatcaag ctcgcccgcc gcgacgcgcc cggccagctc cgcgaggtaa ggcgcgcggt
     1141 atccggcgcg caccacgtcc ctgtagaaat cgagcggctg cgcggccatc acttcggcgg
     1201 tcgggaacgt ccgcccgccc gccttcgacg cacgcccgag tccattgacc aaacccgtca
     1261 ccatcttgcg cgtgagcgac caagagcagt tggtcgtcat gatcgacttg acgaggtctt
     1321 cgtagacggt cggcgagcgg agcaggcggc ccgcgccgtc gcgctcgacc cacgcgaagc
     1381 ccggctccgc cgagagcgcg ccgtagaact ccgtcatgtc gtcgtcgagg cggaacatgt
     1441 gccgcacgtc gcgcaggact tgcgcggcct cgcgcttgcc cagccggcgc gcggcgcgga
     1501 cgttcacgcc gctccggtcg cccgtgatgg tgagcgtcac cggctcgcgc tcgccgaggt
     1561 cgagcacgcg gcgcagctcg ccttcgttga cccactcgca cggcgccagc tcgctccacc
     1621 cgtggctgcc gagcgtgcgc ttgaaatcga aaccgcgcgg cgcgtcgatc ctgaaatcca
     1681 tgctgtcttt cagtcctccg gcgggata
//
LOCUS       DBNGOH010000018         3846 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2568932, whole genome shotgun sequence.
ACCESSION   DBNGOH010000018 DBNGOH010000000
VERSION     DBNGOH010000018.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3846)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3846)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3846
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2568932"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..518)
                     /locus_tag="AC159P_00360"
     CDS             complement(<1..518)
                     /locus_tag="AC159P_00360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3693929.1"
                     /translation="MASTHEDKVWTDEARKLEGIRRDVEEARGEGLSVLVVAHFENML
                     SSVSQRLRECSIEFKTYASGDPSALCARASGGGAPPSVWVVLASHLWTSGHAVAPKSG
                     EVSLRVLVAEHHPLLAKDEALLAALLRLPCRSRVTFHAALNDELLARVVGEGTMNLLK
                     MLGHTDEECISHP"
     gene            complement(524..2191)
                     /gene="hutU"
                     /locus_tag="AC159P_00365"
     CDS             complement(524..2191)
                     /gene="hutU"
                     /locus_tag="AC159P_00365"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011197306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - L-histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HIR3693930.1"
                     /translation="MTQPRIVRAPRGAELSCRGWHQEAALRCLMNNLDPEVAERPEDL
                     VVYGGRGRAARSWGAFDAIVRELRRIENDETLLVQSGKPVAVFRTHDMAPRVLIANSN
                     LVGAWATWEHFHELERRGLMMYGQMTAGSWIYIGTQGIVQGTFETFAAMADRHFAGTL
                     AGRLVVSGGMGGMGGAQPLAATMNGAAFLGIDVDRERIERRVRTGYCDRLALDLDEAL
                     RICEDAVEQQRAVSVGLVGNCAEVLPELVRRGTKIDAVTDQTSAHDPLNGYVPEGLSL
                     EEAEELRKTDAKAYVERSTASMARHVEAMLALQRAGAVAFDYGNNIRKFALDAGCRDA
                     FDIPGFVPEYIRPLFCEGKGPFRWVALSGDAADIRRTDDLALELFPEDETLNRWLRLA
                     RDRVQFQGLPARICWLGYGERAEMGEAINELVRRGELKAPVAIGRDHLDTGSVASPFR
                     ETEAMRDGSDAVADWPILNALLNTASGASWVSFHHGGGVGIGYSLHAGQVSVADGTPE
                     ASRRLNRVLTNDPGLGVARHVDAGYEEAEETARAKGVRVPMLGAGGE"
     gene            complement(2145..2507)
                     /locus_tag="AC159P_00370"
     CDS             complement(2145..2507)
                     /locus_tag="AC159P_00370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3693931.1"
                     /translation="MKPLNGFHRAHTWWVVFLGHQAFLFATAVGFLHSVRRLTGRAVL
                     SGRDPLGVLDGAALVAGFPASGLWHAGAVVEVVFERVAPNRVAVALMPATVAALFALL
                     KRKHDSTKNRPRAAGRRA"
     gene            complement(2708..3037)
                     /locus_tag="AC159P_00375"
     CDS             complement(2708..3037)
                     /locus_tag="AC159P_00375"
                     /inference="COORDINATES: protein motif:HMM:NF024845.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HIR3693932.1"
                     /translation="MADVFDDRRKALEDDYFRRRDKESLAKLREALREEAKAKGEHVA
                     TMPCPRCDGMLHEETFDEVSIDRCDKCNGIWLDAGELEEIIKEENPSGRWLSIFWPGR
                     ARAKDEG"
     gene            3374..>3846
                     /locus_tag="AC159P_00380"
     CDS             3374..>3846
                     /locus_tag="AC159P_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014508799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HIR3693933.1"
                     /translation="MALGPRVRVLLIAAVGFGALAALVWYLSTRFWTQPVAPLAPPSR
                     EAEALPQPSPPTTPTVAPTVEPTATPSPGASPSVAPSPSTAPGASPTQQPSTESPAPP
                     AASGDAGRLLIPVAGVRPEQLSDTYNDARSEGRVHNAIVIIAPRGTPVLAVADGR"
BASE COUNT          612 a         1380 c         1232 g          622 t
ORIGIN      
        1 gggtgcgaga tgcactcttc gtccgtgtgt ccgagcatct tcagaaggtt catggtgccc
       61 tcgccgacga cgcgcgcgag cagctcatcg ttcagcgccg cgtggaacgt cacgcggctg
      121 cgacacggca gccgcaggag cgccgccagc agcgcctcgt ccttcgccaa caacggatgg
      181 tgctccgcga cgagcacgcg caaacttacc tcacctgact tcggcgccac cgcatggccg
      241 ctcgtccaca agtgcgaagc caacacgacc cacaccgacg gcggagcgcc gccgccgctc
      301 gccctcgcgc acagcgccga agggtcgccc gacgcatacg tcttgaactc gatcgagcac
      361 tcgcgcagtc tctgcgacac gctcgacagc atgttctcga agtgcgcgac gacgagcacg
      421 ctcagcccct cgccgcgcgc ctcttccacg tcgcgccgga tgccttcgag cttccgcgcc
      481 tcgtccgtcc aaaccttgtc ttcgtgagtc gaagccatcc acctcactcg ccgcccgcgc
      541 cgagcatcgg gacacggacg cccttcgcgc gcgcggtctc ttcggcttcc tcataacctg
      601 catcgacgtg gcgcgcgacg ccgagaccgg ggtcgttggt gaggacgcgg ttgaggcggc
      661 gcgaggcttc cggcgtgccg tcggcgacgg agacctgtcc ggcgtggagc gagtagccga
      721 tgccgacgcc gccgccgtgg tggaaggaga cccaagaggc gccgctcgcg gtgttgagca
      781 gcgcgttcag gatgggccag tcggcgacgg cgtcggagcc gtcgcgcatc gcctcggtct
      841 cgcggaaagg ggaggcgacc gagcctgtgt cgaggtggtc gcgcccgatg gcgacgggcg
      901 ccttcagctc gccgcggcgg acgagttcgt tgatggcctc gcccatctcg gcgcgctcgc
      961 cgtagccgag ccagcagatg cgcgcaggca agccctggaa ctgcacgcgg tcgcgcgcga
     1021 gacgcagcca gcggttgaga gtctcgtctt cggggaagag ttcgagcgcg agatcgtccg
     1081 tcctgcggat gtctgcggcg tcgcccgaga gcgcgaccca gcggaagggg cctttgcctt
     1141 cgcagaagag cgggcggatg tattcgggca cgaagccggg gatgtcgaaa gcgtcgcggc
     1201 acccggcgtc gagcgcgaac ttgcggatgt tgttgccgta gtcgaaggcg acggcgcccg
     1261 cgcgctgcaa ggccagcatc gcctcgacgt gccgcgccat gctcgccgtc gagcgctcga
     1321 cgtaagcctt cgcgtccgtc ttcctcaact cctctgcctc ttcgaggctc aagccttcgg
     1381 gcacgtagcc gttcagaggg tcgtgcgcgc tcgtctggtc ggtcaccgca tcgatcttcg
     1441 tgccgcggcg gacgagttcg ggaagcacct ccgcgcagtt gccgacgagg ccgaccgaga
     1501 cggcgcgctg ctgttcgacg gcgtcttcac aaatccggag cgcttcgtcc aaatcgagcg
     1561 cgagacggtc gcagtagccg gtgcggacgc gccgctcgat gcgctcgcgg tcaacgtcga
     1621 tgccgaggaa ggcagcgccg ttcatcgtcg ccgccaaagg ctgcgcgccg cccatcccgc
     1681 ccatcccgcc ggagacgacg aggcggcccg cgagagtgcc ggcgaagtgg cggtcggcca
     1741 tcgcggcgaa ggtctcgaac gtgccctgca cgatcccttg cgtgccgatg taaatccagc
     1801 tcccggcggt catctgcccg tacatcatca gcccgcgccg ctccagctcg tggaagtgct
     1861 cccaagtggc ccacgcgccg acgaggttcg agttggcgat caggacgcgc ggcgccatgt
     1921 cgtgcgtgcg gaagacggcg accggcttgc ccgactggac gagcagcgtc tcgtcgttct
     1981 cgattcgtcg cagctcgcgc acgatggcgt cgaacgcgcc ccaactgcgc gccgcccgcc
     2041 cgcgcccgcc gtacacgacg aggtcttcgg gccgctcggc cacctccggg tcgaggttgt
     2101 tcatcaggca gcgcagcgcc gcctcctgat gccagcccct gcaactaagc tcggcgcccc
     2161 gcggcgcgcg gacgattctt ggttgagtca tgcttcctct tgagtaaagc gaacagggcg
     2221 gcgaccgtcg ccggcatcag cgcgacggcg acccggttgg gcgcgacccg ttcgaagacc
     2281 acttcgacaa ccgcgcccgc gtgccacagc ccgctcgccg ggaagcccgc cacgagcgcc
     2341 gcgccgtcca acacgccgag cgggtcgcgc cccgaaagca cggcccgccc ggtcaacctc
     2401 ctgaccgaat gaagaaaccc gacggcggtg gcgaagagga acgcctgatg cccgaggaac
     2461 acgacccacc acgtgtgcgc tctgtggaag ccgttgaggg gtttcatggc ggaagtgaag
     2521 ccgaccggcg aagcctaccc gcgcgcggcg gcgacgacgc ggcgcggcaa agcagcgcac
     2581 gcgtccttcg caacggtcgc cccgaagaga gcagttttcg gttttcggtt ttcagacaga
     2641 gaacccgtct tcgctgacag ctcacggctc atagctgaat gccgaaaacc gaagactgaa
     2701 agccgtctca tccttcgtcc ttcgcgcggg cgcgaccggg ccagaagatg ctcagccagc
     2761 ggcccgaggg gttctcttcc ttgatgatct cttccagctc gcccgcgtcg agccagatgc
     2821 cgttgcactt gtcgcagcgg tcgatgctca cctcgtcgaa ggtctcttcg tggagcatcc
     2881 cgtcgcagcg cgggcagggc atcgtggcga cgtgctcgcc cttcgccttc gcctcctccc
     2941 gcagcgcctc cctcagcttc gccaaagact ccttatccct gcgcctgaag taatcgtctt
     3001 ccagcgcctt cctgcggtcg tcgaaaacgt ctgccacgct tcttcacctc caacgggttg
     3061 cgtatcagcc gcgcgccgcc ctccgccggg cgaccgcgac caggaacagt ctgtgtaatt
     3121 tcgagcctat gtaataacaa aaaagcacgg gccgcgccac tagagcagta ggcagtaggc
     3181 agtaggcagt caaaagaaaa gacgtgcttt gtcgttcgtg atttccaaag gaagctttcg
     3241 aatttcgaat ccacgacttc ccatctgcct gctgcctact gcctacggct tccccacttg
     3301 cttcgcccgc cgcgagttct tatgctctgt tcgtcttcag cacaagttaa gccgacaagg
     3361 tggtgaaaga gcgatggcac tcggcccgcg cgttcgcgtg ctcctgattg cggcggtcgg
     3421 tttcggcgca ctggcggcgc tcgtctggta cttgagcacg aggttttgga cgcagcccgt
     3481 cgcgccgctc gcgccccctt cgcgcgaggc ggaggcgctg ccgcagccct cgccccccac
     3541 gacaccaaca gtggcgccga ccgtcgaacc taccgcgacg ccgtctcccg gcgcgtcgcc
     3601 ctcggtcgcg ccgtcgccct cgaccgcgcc cggcgcgtcg ccgacgcagc agccttcgac
     3661 cgagtcgccc gcgccgcccg ccgcttcggg cgacgccggg cggctgttga ttccggtggc
     3721 gggcgtgcgc cccgaacagc tcagcgacac gtacaacgac gcgcgctccg aggggcgcgt
     3781 ccacaacgcc atcgtcatca tcgccccgcg cggcacgccc gtcctcgccg tcgccgacgg
     3841 tcgcat
//
LOCUS       DBNGOH010000019         5758 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1102567, whole genome shotgun sequence.
ACCESSION   DBNGOH010000019 DBNGOH010000000
VERSION     DBNGOH010000019.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5758)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5758)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5758
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1102567"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="AC159P_00385"
     CDS             <1..327
                     /locus_tag="AC159P_00385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HIR3693934.1"
                     /translation="SPVLEGDHLYGMSSLRKGQYFCLDAKTGKVLWTTEGREGTQAAV
                     VSAGNALLFLTADGNLIVAGKSAKGFEKVAQYTVADSATYAHPAIVGKQILIKDEAAL
                     SLWSVE"
     gene            437..1864
                     /locus_tag="AC159P_00390"
     CDS             437..1864
                     /locus_tag="AC159P_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020470515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIR3693935.1"
                     /translation="MLHIPILRKGEPYRSLDVARAPHSRTREAFVEISQANAGLIRRD
                     LLDQATPRAALRRFTSAELLDICARAAGHFMNDVLAVGEEEQSPEDYVRQLSSTTGLP
                     HSLARRNMARVHGVMTRMGNVLGGLTRGLDLSILDRGAGETGGHALSFFPRGDSLGVV
                     LPSNSPGVHALWIPAVPLKVPLVLKPGASEPWTPYRIIQAFVRAGAPREAFSYYPTDH
                     AGAAEILRRTGRGMVFGDSSTTNVWKDDPRVEIHGPGHSKIVIGDDLVDRWEDYLDVM
                     VASIAENGGRSCVNASGVWVPRHADRIAEALAERLAQIVPRAVDDPLAVLAPFAAPEV
                     AARISRMIDEGLRAEPGARDVSATERGDERLVTLDGATYLLPTVVRCESAAHPMANRE
                     FLFPFASVVEVKPEEIPEAMGFSLVLTAITEDERLIERLIASPLVDRLNVGPVSTMQI
                     AWDQPHEGNLFEHLYSRRAFQRATA"
     gene            1947..3281
                     /locus_tag="AC159P_00395"
     CDS             1947..3281
                     /locus_tag="AC159P_00395"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024968.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR3693936.1"
                     /translation="MRILYLTAGAADMYCGSCLRDNALASELLSRGHDVVLTPIYTPT
                     LTDEANVSGGKVFFGGISVYLEQYVPLFRKTPRFLDRLWDSSWALKLASRRSVSVNPK
                     MLGEMTVSMLRGEDGNQRKELEKMLDWLKTEPPFDVLSLPYTLLIALAKPLKETLERP
                     VFCTLQGEDLFLEGLQEPYRTESLRLIRANVKYVDTFIPVSEYYARFMPGYLGIPAEK
                     MRVLPLGINLEGYEMRAPRPADEPFTVGYFARVAPEKGLHLLAESYRLMREGGRLENA
                     RLEVAGYLGADHKSYLQDIERRMKEWGLAEEFHYRGALDREGKIEFLRSLDVLSVPAT
                     YDEPKGIFLLEAMACGVPVVQPRRGGFTEIVEKTGGGLLVEPDDAQSLAEGILRMRRD
                     RALAEELSARGFRHVREHYSVARMAERALEVYGGAARESAEQSSGVARLGAA"
     gene            3350..3946
                     /locus_tag="AC159P_00400"
     CDS             3350..3946
                     /locus_tag="AC159P_00400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3693937.1"
                     /translation="MEMMVRGKHNIGLTALLFAHVCLLPMCTNVSAQSRNPHRHIKTF
                     GSLLNEVSSDFTIVEVSLAATLKKDSRAANRRKTYAAYTVHHADRQPNFRIYFEEKRT
                     GRIYEIRGLPLAHRPFSDLVWANNRTLVFDRWSQPHYGMHYAVDVKKKRLTVAAPFPD
                     EFYLEQQRPKRGGALLESARPHPGFEIANRKPFSSRSR"
     gene            3971..4639
                     /locus_tag="AC159P_00405"
     CDS             3971..4639
                     /locus_tag="AC159P_00405"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020469882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3693938.1"
                     /translation="MLEVSNLSKSYPTPRGALDILSDVSLTLRRGDAVSIMGPSGSGK
                     STLLYIVGALEPPTAGTVTLDGQNPFRLKERELAAFRNKEIGFVFQDHCLLPQCTVLE
                     NVLTPTLVAPPNGDDAGSRALSLLEEVGLADRLTHRPAELSGGEKQRVALARALIRKP
                     LLLLCDEPTGNLDQAAAERVASLLLELHRKQETILVVVTHSAELAARFPTRFRMIDKR
                     LEKL"
     gene            4782..>5758
                     /locus_tag="AC159P_00410"
     CDS             4782..>5758
                     /locus_tag="AC159P_00410"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3693939.1"
                     /translation="MLMNLAGLVRRNLTYYWRTNLAVVFGVGVAVAVLAGALLVGDSV
                     RASLRGLFLERLGRTEAVVTSAGFFREQLAADVRGHSRFAAGGFEAACPLVALVGAVA
                     HEESGRRGAGLQVYGVDERFWQFHGREGAASRAPRGSEVLLSESLAAELGAKAGDTLL
                     LRIEKPSEIPVESVHGRKEDLGRTLRLTVREALPPAALGEFSVRPQQSAVRAVFVPLG
                     LLQKELKQEGKVNTLLVSAKAADENQAGRTDAAALENILRESFALEDLGITVRALDER
                     RGLALESDSALVNDHLAEAARAAAEKSGMRAAPVLSYLANTISANGREVP"
BASE COUNT         1018 a         1839 c         1916 g          985 t
ORIGIN      
        1 tcgcccgtgc tcgaaggcga ccacctgtac ggcatgtcct cgctgcgcaa aggtcaatac
       61 ttctgcctcg acgcgaagac cggcaaggtc ttgtggacga ccgaagggcg cgaaggcaca
      121 caagccgccg tcgtcagcgc cggcaacgcg ttgctcttcc tgacggcgga cgggaacctg
      181 atcgtcgccg gcaagagcgc gaaaggtttc gaaaaggtcg cgcaatacac cgtcgccgac
      241 agcgccacct acgcgcaccc ggccatcgtc ggcaagcaga ttttgatcaa ggacgaagcg
      301 gcgctttcgc tatggagcgt cgaataaagt actgagggga aaaagtgatg aggactgagg
      361 actgaggact gaggactgag taaaaataaa tcgctttcac tcagtcctca gtcctcagtc
      421 ctcagtactg agttttatgc tgcacatccc gatcctacgc aagggcgaac cgtaccggag
      481 tctcgacgtg gcgcgcgcgc cgcacagccg cacgcgcgaa gccttcgtcg agatcagtca
      541 ggccaacgcc ggtctgatcc gccgcgacct gctcgaccag gcgacgccgc gcgcggcgct
      601 gcgtcgcttc acgtccgccg agttgctcga catctgcgcg cgcgccgccg gtcacttcat
      661 gaacgacgtg ctggcggtcg gcgaggagga gcagtcgccg gaggattacg tccgccagtt
      721 gtcttcgacg acagggctgc cgcactcgct cgcgcgcagg aacatggcgc gcgtccacgg
      781 cgtgatgacg cggatgggga acgtgctggg gggactcacg cgcggactcg acctctcgat
      841 tctggaccgc ggcgcaggcg agacgggcgg ccacgccctg agcttcttcc cgcgcggcga
      901 ctcattgggc gtcgtgctgc cgagcaactc gccgggcgtg cacgcgctgt ggattccggc
      961 ggtgccgctc aaagtcccgc tcgtcctgaa gccgggcgcg tcggagccgt ggacgcccta
     1021 ccgcatcatt caggcattcg tccgcgcggg cgcgccgcgc gaggcgttca gctactaccc
     1081 gaccgaccac gcgggcgccg cagagatact gcgccgcacg gggcgcggca tggtcttcgg
     1141 cgactcttcg accacgaacg tgtggaagga cgacccgcgc gtggagatac acggccccgg
     1201 ccacagcaag atcgtcatcg gcgacgacct cgtagaccgc tgggaagact acctcgacgt
     1261 gatggtcgcc agcatcgccg agaacggcgg gcgctcgtgc gtcaacgcct cgggcgtctg
     1321 ggtcccgcgc cacgccgacc ggatcgccga ggcgctggcc gaacgcctcg cgcagatcgt
     1381 cccgcgcgcg gtcgatgacc cgttggcggt gctcgcgccc ttcgccgcgc ccgaggtggc
     1441 cgcgcgcatc tcgcggatga tcgacgaggg cttgcgcgca gagccgggcg cgcgcgacgt
     1501 gtcggccaca gagcgcggcg acgaaaggct cgtcacgctc gacggcgcga cttatctgtt
     1561 gccgacggtc gtgcgctgcg agagtgcggc gcacccgatg gcgaacaggg aattcctgtt
     1621 cccgttcgcc agtgtggtcg aggtgaagcc cgaagagatt cccgaggcga tgggcttcag
     1681 cctcgtcctg acggccatca cggaggacga gcgcctgatc gaacggctca tcgcctcgcc
     1741 gctcgttgac cggctcaacg tcggccccgt ctcgacgatg cagatcgcat gggatcagcc
     1801 gcacgagggc aacctcttcg agcaccttta ttctcgtcgc gcgttccagc gggcgacggc
     1861 gtaattcaag ctgttagctc ttagccgtta gctttcagct tttcgttttt gctaacggct
     1921 aagagctaac agcttatagc tttctcatgc gtattcttta cctgaccgcc ggggccgccg
     1981 acatgtactg cggcagttgc ctgcgcgaca acgcgctcgc gagcgagctg ctgtcgcgcg
     2041 ggcacgacgt ggtgctgacg ccgatctaca cgccgacgct gacggacgag gcgaacgtga
     2101 gcggcggcaa ggtcttcttc ggcggcatca gcgtctacct cgaacagtac gtgccgctct
     2161 tccgcaagac gccgcgcttt ctcgataggc tgtgggactc ttcgtgggcc ttgaagctgg
     2221 cgtcgcgccg ttcggtctcg gtcaatccga agatgctcgg cgagatgacc gtctcgatgc
     2281 tgcggggcga ggacggcaac cagcgcaaag agctggaaaa gatgctcgac tggctgaaga
     2341 ccgaaccgcc gttcgatgtc ctcagcctgc cgtacacact gctcatcgcg ctcgccaagc
     2401 ctttgaagga gacgctcgag aggcccgtct tctgcacgct gcaaggcgaa gacctcttcc
     2461 tcgaaggctt gcaggagcct taccgcacgg agtccttgcg gctgatccgc gccaacgtga
     2521 agtacgtgga cacgttcatc ccggtgagcg aatactacgc gcgcttcatg ccgggctacc
     2581 tcggcatccc ggcggagaag atgcgcgtgc tgccgcttgg catcaacctc gaaggctacg
     2641 agatgcgtgc gccgcgcccg gctgatgaac ctttcaccgt cggctacttc gcgcgcgtcg
     2701 cgccggagaa gggcctgcac ctcttggccg aaagctaccg gctcatgcgc gagggcgggc
     2761 gattggaaaa cgcgcggctc gaagtcgcgg gctacctcgg cgcggatcac aaaagttatc
     2821 tgcaagacat cgagcggcgg atgaaggagt ggggtctcgc ggaagagttc cactaccgcg
     2881 gcgcgctcga ccgtgagggg aagatcgagt tcctgcgcag cctggacgtg ctctccgtgc
     2941 cggcgaccta cgacgagccg aagggcatct tccttttgga agcgatggcc tgcggcgtgc
     3001 ccgtcgtcca gccgcgccgc ggcggcttca ccgagatcgt cgagaagacc ggcggcggcc
     3061 tgcttgtcga gccggacgac gcgcaatctc tggccgaagg catcctccgc atgcgccgtg
     3121 accgcgcgct cgccgaagag ttgagcgccc gaggctttcg ccacgtgcgc gagcactaca
     3181 gcgtcgcccg catggcggag cgcgcgctcg aagtctacgg cggcgccgcg cgcgagagcg
     3241 cggagcagtc gagcggcgtg gcgcggctgg gtgcggcttg agccgggtga gagggaatcg
     3301 aagcggagcc gggcaggacg agacggaaag tattcgccgg tcgaattgtg tggagatgat
     3361 ggtcagggga aagcataaca tcggtctcac ggctctactc ttcgcgcacg tgtgcctgct
     3421 gccgatgtgc acgaacgtat cggctcaatc tcgtaatcct caccggcaca tcaaaacatt
     3481 cggttcgttg ctgaatgaag tttcttcgga tttcacgatt gtagaggtgt cgctggcagc
     3541 gactttgaag aaagacagcc gcgcggcgaa tcgtcgaaaa acctacgcgg cctataccgt
     3601 tcatcacgcc gaccggcagc cgaatttccg tatctacttc gaagagaagc gcacgggcag
     3661 gatttacgag atacgcggct tgcctctcgc gcaccgtccg ttctcggatt tggtctgggc
     3721 gaataaccgc acgctggtgt tcgaccggtg gtcgcagccg cattacggta tgcactacgc
     3781 cgttgacgtg aagaagaagc ggctgactgt cgccgcgcct ttccccgacg agttttacct
     3841 cgagcagcaa agaccaaagc ggggcggcgc tttgttagag agcgcgaggc cacatccggg
     3901 ctttgaaatc gcaaatcgca aaccattctc atcacgttca cgttaatctt catgttcgag
     3961 gaatcgacgc ttgctcgaag tctctaacct ctcgaagtcg tacccgacgc cgcggggcgc
     4021 gctcgacatc ctgtcggacg tgtcgctgac gctcaggcgc ggcgacgccg tctcgatcat
     4081 ggggccgtcg gggagcggca aatcgacgct gctctacatc gtcggcgcgc tggagccgcc
     4141 gaccgcaggc accgtcacgc tcgacgggca gaaccctttc cggctgaaag agagggaact
     4201 cgccgcgttc aggaacaaag agatcggctt cgtctttcag gatcactgtc tgctgccgca
     4261 gtgcacggta ctggagaacg tgctgacgcc gacgctggtc gcgccgccga acggcgacga
     4321 cgccggaagc cgcgcgctct ccctgctcga agaggtgggc cttgccgacc ggctcacgca
     4381 ccgcccggcg gagctgtcgg gcggcgagaa gcagcgcgtg gcgctcgcgc gcgcgttgat
     4441 cagaaagccg ctgctcctgc tctgcgacga gccgacgggc aacctcgatc aggccgcggc
     4501 ggagcgcgtc gcctcgctcc tgctcgaact ccaccgcaag caggagacca tcctcgtcgt
     4561 cgtcacgcac agcgccgagc tggccgcacg cttccccacg cgattcagga tgatcgataa
     4621 aagactggaa aaactttaag gggaaaaggt taaaagggga aaaggttaaa agggaagacg
     4681 aaaggcacgg tgcctctcgt caacttgacg atgagaaaca gcgtgccttt tcatcctccc
     4741 ttttcgccct ttcccctttt aaccttttcc cctctgtctt catgcttatg aatctcgccg
     4801 gtctcgtccg acgcaatctc acttactact ggcgcacgaa cctggcggtc gtgttcggcg
     4861 tgggcgtggc cgtcgcggtg ctggcgggcg cgctgctcgt cggcgactcc gtgcgcgcgt
     4921 cgctgcgcgg gctgttcctc gaaaggctgg ggcgcaccga agcggtcgtc acgtccgcgg
     4981 gcttcttccg cgaacagctc gcggcggacg tgcgagggca cagccggttc gccgctggag
     5041 ggttcgaggc ggcgtgcccg ttggtcgcgc tggtgggcgc ggtcgcgcac gaggagagcg
     5101 ggcggcgcgg cgcggggctt caggtctacg gcgtggacga gcgcttctgg cagttccacg
     5161 ggcgcgaggg cgcggcgtcg cgcgcgccgc gcgggagcga ggtgttgttg agcgaaagcc
     5221 tcgcggcgga actcggcgcg aaggcgggcg acacgcttct gctgcgcatc gagaagcctt
     5281 cggagattcc ggtcgaatcg gtgcacgggc gcaaagagga tttgggccgc acgctgcggc
     5341 tgacggtgcg cgaggcgctg ccccctgcgg cgctcggaga gttctcggtg aggccgcagc
     5401 agtcggcggt gcgcgccgtg ttcgtcccgc tcgggctgct gcaaaaggag ttgaagcagg
     5461 agggcaaggt caacacgctg ctcgtctccg cgaaggccgc cgacgagaat caggccgggc
     5521 ggacggacgc cgcggcgctc gaaaacattt tgcgcgagag cttcgcgctc gaagatttgg
     5581 gcatcaccgt gcgggcgctc gacgagcggc gcggcctcgc gctcgaaagc gacagcgccc
     5641 tcgtcaatga ccacctcgcg gaggccgccc gcgccgccgc cgagaagtcg gggatgcgcg
     5701 ccgcgcccgt cctctcttac ctcgccaaca cgatcagcgc gaacggacgc gaagtgcc
//
LOCUS       DBNGOH010000020         4791 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_369590, whole genome shotgun sequence.
ACCESSION   DBNGOH010000020 DBNGOH010000000
VERSION     DBNGOH010000020.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4791)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4791)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4791
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_369590"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            165..782
                     /locus_tag="AC159P_00415"
     CDS             165..782
                     /locus_tag="AC159P_00415"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012863.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HIR3693940.1"
                     /translation="MGDGEQRGPLVYAALGDSTGAGVGAREGGGYVARLFERIERARP
                     GSRLVNLSVSGATTEHVLFQQVGRLLEGGVPSLVTLGIGINDVQRLMLEEYERNLEQI
                     VRRVRAATDAPFVLTNIPDITCSPAVPEFMRAEARKRIDAFNAALARVAALHSLTLVD
                     VYTKSRELLPARPDFFCSDLFHPSDEGYRFWADEMWPAVEQAIEQ"
     gene            980..1267
                     /locus_tag="AC159P_00420"
     CDS             980..1267
                     /locus_tag="AC159P_00420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3693941.1"
                     /translation="MTDTNDPRYKTGNPLCDALRFVCDASFAVLPADAARQLGEFEKN
                     LWGGLRWFAEKNIRWIDESLDAADRLRAEWQSRRDTPPPPPPAPDSDPVVI"
     gene            1412..2260
                     /gene="ispH"
                     /locus_tag="AC159P_00425"
     CDS             1412..2260
                     /gene="ispH"
                     /locus_tag="AC159P_00425"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: protein motif:HMM:NF002187.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HIR3693942.1"
                     /translation="MQVLLADEYGFCFGVERAVEMVEDELAEGATVRTLGPLIHNTQE
                     TQRLEAEGVRTIDAPAEVEAGVTAVIRAHGVTPAVQRELETRATKVVDATCPFVTKVQ
                     KLAERAAAQGRDVVVVGNPDHPEMIGVVGYAPHHSHVVRDASEVAALPDLHAPLVVSQ
                     TTLKLKTFLEAAEAVRLKADAEPQIVNTICSATRDRQDAARALAGEVDAFYVIGGKHS
                     SNSVKLLAVCQEQCPKSFLIETPGEIDPADLAGAERVGVTAGASTPNWLIEQVVKRLR
                     EIGDAK"
     gene            complement(2358..2996)
                     /locus_tag="AC159P_00430"
     CDS             complement(2358..2996)
                     /locus_tag="AC159P_00430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3693943.1"
                     /translation="MIEDFGTRVASVWEGLRPVTRSLVERAVLAAQTPAGVNAATVSP
                     RAGASYDPRSEWELSRLLAALDERAVEKGAVVLSVEQSTELVRISETCAAVLHQEART
                     AEVFAQLLERAFRSRDYRRVDTLADTLAERLAPSETCELARSENPMVRAIAHETLLQT
                     PVAVLVALLADPVDAEIARAALEYQAAEYGTEEARMIVSALDLADAADDEDL"
     gene            complement(3188..3406)
                     /locus_tag="AC159P_00435"
     CDS             complement(3188..3406)
                     /locus_tag="AC159P_00435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3693944.1"
                     /translation="MEVNKALATLQPLYGKSNVEIVTDDGSRVRGLVRSLRTTQALTR
                     PSVKIERADGQVVKIPFANITQIIDHNR"
     gene            3768..4730
                     /locus_tag="AC159P_00440"
     CDS             3768..4730
                     /locus_tag="AC159P_00440"
                     /inference="COORDINATES: protein motif:HMM:NF024578.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HIR3693945.1"
                     /translation="MPDGLRFCRKCGFRLGEGVEEYTATRRFDAPPATAPAATATTTN
                     LNGAGAAFNMPGTWGAVAPVSAASMQGQTDSKFMKLASSCNPLRLNWIVWILVLTAVL
                     TAGTLVVQKMTRSGRFAPPPPAVSFVGVDGFSTADGGGAFIDGIAGVGSPVERAGLIG
                     GDIITSINGQKIEDADDASRIIRATPPGQTIEVVYIRDGATATTQLTTITKKEFAGLE
                     PLEARPGGQGRIGVDVGSRKRVPTLNTYGVELDDVDRNGPADLAGLKEGDIVIQFNEH
                     LVRTAGDLRYRIWAAVPGSTAEVIVMRGSERVAVPVKVGRSKDP"
BASE COUNT          826 a         1606 c         1585 g          774 t
ORIGIN      
        1 ctcgcgccga aatgagtttt caaacaggct ctaagcaatt tggattgcgg attacggact
       61 ggcgtgccgc tcggacttga agcttgattt cgaacgtgta actttggtcg ctcgataaat
      121 ccgcaatccg caatccgcaa tccaaaatcg gaagggggaa agagatgggc gacggagagc
      181 agagggggcc gttggtgtac gcggcgctcg gggattcgac gggcgcgggc gtcggcgcgc
      241 gcgagggggg cggctacgtg gcgcgtctgt tcgagcgcat cgagcgggcc cggcccggct
      301 cgcggctcgt caatctgtcc gtgagcggcg cgaccacgga gcacgtgctg ttccaacagg
      361 tcgggcgcct cttggagggc ggcgtgccgt cgctcgtcac gctcggcatc ggcatcaacg
      421 acgtgcagcg gctgatgctc gaagagtacg agcggaacct ggaacagata gtccgccgcg
      481 tccgcgccgc gaccgacgcg cccttcgtcc tgaccaacat ccccgacatc acctgctcgc
      541 ccgccgtccc cgaattcatg cgcgcagagg cgcgcaaacg catcgacgcc ttcaacgccg
      601 ccctcgcccg cgtcgccgcg ctccactcgc tcaccctcgt cgatgtctac acgaagagcc
      661 gcgaactgct ccccgcgcgc ccggacttct tctgctccga cctcttccac ccgtcggacg
      721 aaggctaccg gttctgggcc gacgagatgt ggcccgcggt cgagcaagcc atcgaacagt
      781 aggcggtagg cagttaaaag aaaaaccgaa tttcattcgg gatgtgaaat ctcgactttg
      841 aaattcgagt tttcttaact gcctaccgcc tactgctttc tgccttttgc cttttgcctt
      901 cggccttcat tatgatgtct cggggaacgc gcggggcctt cgggccgtat ggcttgggta
      961 aggaggtctg gaatcatcga tgactgacac gaacgaccca cgctacaaga ctgggaaccc
     1021 gctgtgcgac gcgctgcgct tcgtgtgcga cgcctcgttc gcggtgctgc cggcggacgc
     1081 ggcgcgccag ctcggcgagt tcgagaagaa tctctggggc ggcctgcgct ggttcgcaga
     1141 gaagaacatc cgctggatcg acgagagcct cgacgccgcc gaccgcctgc gcgccgagtg
     1201 gcaaagccgg cgcgacacgc cgccgccgcc gcccccggcg ccggacagcg acccggtggt
     1261 gatttgaaga agcgattagc cgttagccgt tagctgtcag ctttcggatt gatagttggc
     1321 gattgatgac agcggcgctc ccatcaatcg tcatccgcca agccaagagc taacagctaa
     1381 tcgctaacag ctaacagcaa aggtaacatt gatgcaggtt cttttagccg acgagtacgg
     1441 gttctgtttc ggcgtcgagc gcgccgtcga gatggtcgaa gacgagcttg ccgagggcgc
     1501 gaccgtgcgc acgctggggc cgctcatcca caacacgcag gagacgcaac ggctcgaagc
     1561 cgagggcgtc cgcaccatcg acgcgcccgc cgaggtcgaa gcgggcgtca ccgccgtcat
     1621 ccgcgcgcac ggcgtcacgc ccgcggtgca gcgcgagctt gagacgcgcg ccacgaaggt
     1681 cgtggacgcg acctgcccgt tcgtcacgaa ggtgcagaag ctggccgagc gcgccgccgc
     1741 gcagggccgc gatgtcgtcg tggtcggcaa ccccgaccac cccgagatga tcggcgtcgt
     1801 cggctacgcg ccgcaccact cgcacgtggt gcgcgacgcg agcgaggtcg ccgcgctgcc
     1861 cgatttgcac gcgccgctcg tcgtctcgca gacgacgctc aagctcaaga ccttcctcga
     1921 agccgccgag gccgtgcgcc tcaaggccga cgccgagccg cagatcgtca acaccatctg
     1981 ctcggccacg cgcgaccggc aggacgcggc gcgcgcgctc gccggcgagg tggacgcctt
     2041 ctacgtcatc ggcggcaagc actcctcgaa cagcgtcaag ctgctcgccg tctgtcagga
     2101 gcagtgcccg aagagcttcc tcatcgagac gcccggcgag atcgaccccg cagacctcgc
     2161 aggcgccgag cgcgtcggcg tcaccgccgg ggcctccacg cccaactggc tcatcgaaca
     2221 ggtcgtcaag cgcctgcgcg aaatcggcga cgctaaataa agaggggcga aaaggttaaa
     2281 aggggaaaag gcgaaaagga ggagcttgtc tcccttcctt ttcgcctttt ccccttttaa
     2341 ccttttcgcc ccttttccta gaggtcttcg tcgtcggcgg cgtcggcgag gtcgagcgcg
     2401 ctgacgatca tgcgggcctc ttcggtgccg tactcggcgg cctggtattc gagcgcggcg
     2461 cgcgcgatct ccgcgtccac ggggtccgcg agcaaggcga cgaggacggc gaccggcgtt
     2521 tgcagcagcg tctcgtgcgc gatggcgcgc accatcgggt tctcgctgcg cgccagctcg
     2581 caggtctcgc tcggcgcgag cctttcggcg agcgtgtcgg cgagcgtgtc cacgcggcgg
     2641 tagtcgcgcg agcggaaggc gcgctcaagg agctgcgcga agacctccgc cgtgcgcgcc
     2701 tcctggtgga gcacggcggc gcaggtctcg gagatgcgaa ccagctcggt ggactgctcg
     2761 acgctcagca cgaccgcgcc cttctccaca gcgcgctcgt cgagcgccgc cagcaggcgg
     2821 ctcaactccc actcggaacg cgggtcgtac gaggcgcccg cgcgcggcga gacggtcgcg
     2881 gcgttcaccc ccgcgggcgt ctgcgccgcc agcaccgcgc gctcgacgag gctccgtgtc
     2941 acgggcctca gaccctccca cacactcgcc acgcgcgtcc cgaaatcttc gatcatcatc
     3001 ttgcttcgaa ctcacgacgc ccgtccgggg ccggcgcggg cctcaccgcc cgcgcgcgta
     3061 atatatcagt aaaagccgtg cagcgtgaac cgtaaaaagc cgtgaaccgt aaaccgcgag
     3121 ccgtgagaag ggaaggcagg ttttaactcg tcacggctca cggtttacgg cccgcggctt
     3181 tcggccttta ccggttgtgg tcgatgatct gggtgatgtt ggcgaagggg attttgacga
     3241 cctggccgtc ggcgcgttcg atcttgacgc tgggcctggt gagggcctgc gtggtgcgca
     3301 ggctgcggac gaggccgcgc acgcggctgc cgtcgtcggt cacgatctcg acgttgcttt
     3361 tcccgtagag cggttggagg gtggccagag ctttgttgac ttccactgtt tttatcgtcc
     3421 cttcccggct atggagtgcg tcaatgtctg tcgccgtggc ttcgtggcac gcgccgtgcc
     3481 gctgcggccc gcaccgggcg tgccgcgcgc ccggtgcgga ctttctccgc gtaaagacgg
     3541 ggccgcgcag cgcctcggcg taaggcgcga gtctatgcgg cgtactaaca agatgcaacc
     3601 gcaccgctcc acgaggcgta taaaccctac agccaagacg cgcctgacga gtatcaacgg
     3661 ggcggctctt acgctagaat aaagacgcca tgacgaacgg gaaccgagac gccgagatgt
     3721 acgcgggagg tgtgggaatg caaccgtgcc cgagctgccg cacgccgatg cctgacggcc
     3781 tgcgcttctg ccgcaagtgc ggcttccgtc tgggcgaggg cgtcgaggag tacaccgcga
     3841 cgcgccgctt cgacgcgccg cccgccaccg cgcctgccgc caccgcgacg accaccaacc
     3901 tcaacggggc gggcgccgcc ttcaacatgc cgggcacgtg gggcgcggtc gcgcctgtgt
     3961 ccgccgcctc catgcagggg cagaccgact ctaagttcat gaaactcgcg tcatcctgca
     4021 acccgcttcg cctgaactgg atcgtctgga ttctggtgct gaccgcagtg ctcacggcgg
     4081 gcaccctcgt cgtgcagaaa atgacgcgga gcggcaggtt cgcgcctccg cctccggcgg
     4141 tctccttcgt cggcgttgac ggcttcagca cggcggacgg cggcggcgcc ttcatcgacg
     4201 gcatcgcggg cgtcggctcg cccgtcgagc gcgcgggcct catcggcggc gacatcatca
     4261 ccagcatcaa cggccagaag atcgaggacg ccgacgatgc cagcagaatc attcgcgcga
     4321 cgccgcccgg ccagaccatc gaggtcgtct acatccgcga cggcgcgacg gcgacgacgc
     4381 agctcacgac catcaccaaa aaagagttcg cgggcttgga gccgctcgaa gcgcggcccg
     4441 gcgggcaggg gcgcatcggc gtggacgtgg gcagccgcaa acgcgtgccg accctgaaca
     4501 cctacggcgt ggagctggac gacgttgacc gcaacgggcc tgccgacctc gccggcttga
     4561 aagaaggcga catcgtcatc cagttcaacg agcacctcgt ccgcacggcg ggcgatttgc
     4621 gctatcgcat ctgggcggca gtgccgggca gcaccgccga ggtcatcgtc atgcgcggca
     4681 gcgagcgcgt cgccgtcccc gtgaaggtcg ggcgcagcaa agacccgtga aggtcgggcg
     4741 cagcagagac cgagcgcacg cgacacggga aggccaggag gtggggcgcg g
//
LOCUS       DBNGOH010000021         3333 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4402170, whole genome shotgun sequence.
ACCESSION   DBNGOH010000021 DBNGOH010000000
VERSION     DBNGOH010000021.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3333)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3333)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3333
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4402170"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1223)
                     /locus_tag="AC159P_00445"
     CDS             complement(<1..1223)
                     /locus_tag="AC159P_00445"
                     /inference="COORDINATES: protein motif:HMM:NF024583.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HIR3693946.1"
                     /translation="MKPRLEAVAGPLKGAAFALGDEEVSVGRDSSNLIPVGDSLLSRR
                     HCLIKQEGKRFKVVDLGSVNGTSVNGVPVKEHFLEQGDQITVGESRFVFLLRGGVPQR
                     DSNPVHLSEQGLSAGRTVRLRVGDALYLQPQDKWSSLPPLGRMARDLTVLVRVSTAVN
                     AVRGVDALQRELLQLIFEVVPAERGAILLAGDGLEITSVFGWDRAEGPERPVSVSRKV
                     VRQVLEEGVAVLSNDAVEGAETGRLESNAATRISSLLCVPLTLFGRALGVVYLDTADA
                     GARFCEDHLHLVTVISGIAAVALENARHVERLEGENLLLRADQRIEHNMIGEGEAMRR
                     VYRFISRVAPADSTVLVTGESGTGKELAAHAIHRNSARREGPFVAINCAALTESLLES
                     ELFGHERGAFTGAVA"
     gene            1736..2209
                     /locus_tag="AC159P_00450"
     CDS             1736..2209
                     /locus_tag="AC159P_00450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3693947.1"
                     /translation="MKKIACVGLLLMSFFVSLPAGASASAQPADELVAEIPFDFYVGG
                     KTMPAGEYTVRTVTTSDDSGLQLRSAAGSESVIILTHAAQARSRRPGPARLVFNRYGD
                     QRFLSVVWRSGGDGRELRLSKRERNLRKELRVAQSGGPAAPATEVVTVVARLEKD"
     gene            2663..3010
                     /locus_tag="AC159P_00455"
     CDS             2663..3010
                     /locus_tag="AC159P_00455"
                     /inference="COORDINATES: protein motif:HMM:NF013868.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HIR3693948.1"
                     /translation="MADLCIERRRAGGVTVLDLRGDIVIGSGGQEISGDLRRLVRGGE
                     RKVLLNLAGTRFVDSSGLGHLLACHNLMRDEGGQLKLLRLNERVRYVMVITKLVTVFD
                     VYDKEEEALSSYE"
BASE COUNT          594 a         1063 c         1120 g          556 t
ORIGIN      
        1 tgcgcgacgg cacccgtgaa cgcgccgcgc tcgtggccga agagttccga ttcgagcagc
       61 gactcggtca gggcggcgca gttgatggca acgaaaggcc cctcgcggcg cgcgctgttg
      121 cggtggatgg cgtgggcggc gagttccttg cccgtgcccg actcgcccgt gacgaggacg
      181 gtcgagtccg ccggggcgac ccgcgagatg aaccggtaga ccctccgcat cgcctcgccc
      241 tcgccgatca tgttgtgctc gatccgctgg tcggcgcgca gcagcaggtt ctcgccctcg
      301 agccgctcca cgtgccgcgc gttctccagc gcgaccgcgg cgatgccgga gatgaccgtc
      361 acgagatgca gatgatcttc gcaaaagcgt gcgcccgcgt cggcggtgtc gaggtagacg
      421 acgccgagcg cgcgtccgaa cagcgtcaac ggcacgcaga ggagcgagct gatgcgcgta
      481 gcagcgttac tctccagccg ccctgtttcg gctccttcga cggcgtcgtt actgaggacg
      541 gccacgccct cttccaggac ttgccggacg actttgcggc ttaccgacac cgggcgctcc
      601 ggcccctccg cgcgatccca gccgaagacg gaggtgattt cgaggccgtc gccggcgagg
      661 aggatcgcgc cgcgctcggc gggaacgacc tcgaagatga gttgcaggag ttcgcgctgg
      721 agcgcatcca ccccgcggac ggcgttgacc gccgtgctga cacggacgag caccgtcagg
      781 tcgcgcgcca tgcgcccgag tggagggagc gaggaccact tgtcttgtgg ctgcaggtag
      841 agggcgtcac cgactcgcag gcgcacggtc ctgcccgccg acagaccttg ctcgctcagg
      901 tggacggggt tcgagtcccg ctgcggcacg ccgccgcgca ggaggaagac gaagcgcgat
      961 tcgccgaccg tgatctgatc gccttgttcg aggaagtgtt ccttgacggg cacgccgttg
     1021 acggacgtac cgttcacgct tcccaggtca acgaccttga agcgtttccc ctcctgctta
     1081 atcaggcagt gccgccgcga gagcaggctg tcgccgacgg ggatcaggtt tgaagaatcg
     1141 cgcccgacgg agacttcttc gtcgcccagc gcgaaggcag cgcccttcag cggccccgcg
     1201 acggcctcga gccttggctt cactgcagag tcgtccgtca tcgcctttaa gcagccgcca
     1261 gcccgcgctc tagcggtagc gcgcgagaaa tatcactttg cccgccaaat gtcacgggaa
     1321 caatgttcac cgggcgagac gcgaacaagt accggaagtg gccgtgtcgc gctcgcccgg
     1381 gctctgtacc cgtctataac gcggtcgaag agctatccga aaagataggc agccaaactt
     1441 tccggatagg gattgcgcga ctagccgcac acgcccgccc gcccgccgag ggttccacgg
     1501 gccgccttaa accgaatcgt cgaagcaatt ccgacgatgg agacactccg acgggcgcgc
     1561 ctcgtgtcgc ccggcacggc gcttgtcata aagagtcggc gtagaggttg tccccaggca
     1621 acccggtttg aaaatggtgg cgtgatggcc gggtcagggg ccgcagttac caacccgaag
     1681 atggcgggtg gcttcatcga tcgcttaccc gccaaacccc aaaaggagaa atgcgatgaa
     1741 gaagatagcc tgcgtcggtc tcctgctcat gagtttcttc gtgtcgctgc cggcgggcgc
     1801 ttccgcctcc gcgcagcccg cagatgagct tgtggcggaa atcccgttcg atttctacgt
     1861 gggcggcaag acgatgcccg ccggcgagta taccgtccgg accgtcacca cctccgacga
     1921 cagtgggctg cagcttcgca gcgccgccgg gagcgagagc gtgatcatcc tgacgcacgc
     1981 ggcacaggcg aggagccggc ggcccggccc ggctaggctg gtcttcaaca ggtacgggga
     2041 tcagcgcttc ctgtcagtcg tgtggcggtc ggggggcgat ggacgcgagt tgaggctctc
     2101 caagcgcgag cgcaacctgc ggaaagagtt acgggtggcg cagagcggcg gcccggcggc
     2161 cccggcgacg gaggtcgtca ccgtcgtcgc gcgcctcgaa aaggattaga agtgtccctg
     2221 cgcagggcac acgccggcca accaacgcac ccacctttac tgagttcggc acgcggcagc
     2281 cgccggaacc cttgtgccgg agctgccgcg ttttccctct cagccccccg acgcaaaggt
     2341 ccagcgagag ggcggcaggg gtggggctga acgacccacc ccgcctcaaa ccggctgcgc
     2401 accgacctcg cttcggagtt ttaacgcgga gggtcggacg cccccaacga gtcagtggcg
     2461 tatcgaattc ggtatttgaa tcggtgtgcc agatacacgc tgactcggtt gatccagacc
     2521 gaaatcagcc gcacggggac tagcattctt gtgcggcgtc gtgatccctc agacaacgct
     2581 tcgacttacc ccgaaacgtc cctgcggact tccgcatata tcagtgggac cgccccgggg
     2641 tagcggaggc ggaggtggtg agatggcaga cttatgtatc gagcggcgcc gggcgggcgg
     2701 cgtgacggtc ctcgatttgc ggggcgacat cgtcatcggc tcgggcgggc aggagatcag
     2761 cggggatctg cggcgtctgg tgcgcggcgg cgagcgcaag gtcctgctga acctggccgg
     2821 cacccggttc gtggactcga gcgggctcgg tcacctgctc gcgtgccaca acctgatgcg
     2881 ggacgagggc gggcagctga agctgctgcg gctcaacgag cgggtgcgct acgtcatggt
     2941 gatcacgaag ctggtgacgg tcttcgacgt ctacgacaag gaggaggagg cgttgtccag
     3001 ttacgaatga atggccggcg gtgtcccgcc gaacccagcc ccggtagttt atttgccgtg
     3061 tcgcgaacac agctcttctc ggaaccgtgc ataactaccc tttcgcgaca atactgctgg
     3121 atcggcgctc cccctggttt gatggtcggg atcatgccct taggggataa agcgaggcaa
     3181 gaacaatgac agacgagcgt gtcgcaatgt ttgtcagcca caaggttgta agtcaccggc
     3241 gggccgcccg ccggataaag gagattctcg aatcgcgcgc agagcgcctg gacgtttaca
     3301 cctgcgaaga gacgcctgcg ggcgaccgct ggc
//
LOCUS       DBNGOH010000022         6071 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4585487, whole genome shotgun sequence.
ACCESSION   DBNGOH010000022 DBNGOH010000000
VERSION     DBNGOH010000022.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6071)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6071)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6071
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4585487"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..509)
                     /locus_tag="AC159P_00460"
     CDS             complement(<1..509)
                     /locus_tag="AC159P_00460"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF024575.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003689 - DNA clamp loader activity
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit"
                     /protein_id="HIR3693949.1"
                     /translation="MFDSLIGNKRAKETLRRMLGQGRVPGALLFAGEEGLGKKLFALE
                     LARALNCVRKVGIEGCGKCPACVRTAHFEFPSSDDKDAHRKVIWSGHRDVGQLIPYNR
                     STLVDAVRDLNDESNYRPFEGAARVFIVAEADSLNEAASNALLKTLEEAPPTTHLVLI
                     TARPSSLLPT"
     gene            complement(563..1228)
                     /gene="tmk"
                     /locus_tag="AC159P_00465"
     CDS             complement(563..1228)
                     /gene="tmk"
                     /locus_tag="AC159P_00465"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012611193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - dTMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HIR3693950.1"
                     /translation="MAAAFITFEGIDGSGKSTQMRLLASELRLRGQEVVSTREPGGTA
                     LGSRIRELVLDNEEQVDPLAELLLYAADRAQHVRTLVRPALESGHVVLSDRYADATAA
                     YQGAGRGFPEALITELIVLATGGLMPDLTLVFDLTVEESRRRARKRTRGETTQKKHDR
                     LDAEDAAFHTRVRDAYLKLAAADPERVRLIDASGSIQETQAQVMKLVIPFIEAGGQRS
                     EVS"
     gene            complement(1293..1592)
                     /gene="hfq"
                     /locus_tag="AC159P_00470"
     CDS             complement(1293..1592)
                     /gene="hfq"
                     /locus_tag="AC159P_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013579535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HIR3693951.1"
                     /translation="MDTKATPQNIQDAFLNTVRREKDTVTIYLMNGAKLSGRIKSFDK
                     FSVLLESGAQEQLIFKHAISTIIHTRRPAGEHRAAAHASAEAAADAGPDQPGRAD"
     gene            complement(1761..2705)
                     /gene="miaA"
                     /locus_tag="AC159P_00475"
     CDS             complement(1761..2705)
                     /gene="miaA"
                     /locus_tag="AC159P_00475"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HIR3693952.1"
                     /translation="MSETEKSVRPVVAVAGPTASGKSDLGIALALRFGGEVVNCDSVQ
                     VYREIEIATAKVPLAERRGVPHHLIDFVAPDVTYTAADWAREAVRTIDEIEARGHLAV
                     LVGGSGFYLRALRRPFFASTPTDADLRQRLAALRERRGAEHLHRMLARLDPVTAARLH
                     ARDWSRVQRALVVYFQTGTPLSLQQGARPEPPPHAARLRLFALNPPRAELYRRIDART
                     ELHFRAGLVEEVRRLLERGVPAASSALGAHGYRRVVEHLRGTRSFASAVEQTKLDVRH
                     YAKRQLTWFRREPGVEWVEGFGEDPAVQAHVASLLARV"
     gene            complement(2702..2881)
                     /locus_tag="AC159P_00480"
     CDS             complement(2702..2881)
                     /locus_tag="AC159P_00480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3693953.1"
                     /translation="MTMDWSGTAIKRQPSSPSTNPQSAIRNPMRFSRMQLVGALFVLL
                     LIWAVVFYRLASFAS"
     gene            complement(2921..5290)
                     /locus_tag="AC159P_00485"
     CDS             complement(2921..5290)
                     /locus_tag="AC159P_00485"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HIR3693954.1"
                     /translation="MRTSTLTRSLALVFAHLLLFAPLAFTPRGTAQENTRPRRVTRPD
                     AGARTESTPAPPAETKNARDATDARARIEGVAKERDDDDLEDRIEQEPSVRIGLTTTA
                     RSVTISTTGRLLDTSNPSAPPAPFETARVRIEPRSMPPLTPTPPPPTEDEALAGLAAE
                     ETAAPGNIAVVNPASARRGTPNANGTYNANGINTAKAGNGTRGSLRLVSRVSEATRGA
                     VLYAPGATQPLADVRAPVTFASDDEAQHPVRYNEKPYRGRLEVFANTRGALTVVNVVP
                     LEDYLRGVVPNELSPGGFPELEALKAQAVAARTYALANLRRFQSAGFDLLPTTRSQVY
                     GGRATEHPLTDRAVAETRGRVATYKGEAINAVYTSTCGGRTEHAEHIFGGHAAPYLRA
                     RECAYESGEPFAPHTVRTARPLFDIKLAEHASSARDAALLTVHGLMSSQRLDDEWLAA
                     PVTVGEVTALLERVAVHTRQLFFSVPNEATKPPAFATALSKALDGESRGDVLLNHLDV
                     AYQLAFKDADEIPDRDRANVAALLRDGHLSLFPDATLRPRQPMTRARVLRAVAHAFEA
                     RGLFRLQKATARIDAGGALIFRLPGKSGDKTLTFMPGGALLFRAFGDALYQVREVSLV
                     GGEPLVFHTDQRGEVSYLEVQPAPNGAAPDRFSAYSNWTETLSPAAAQSRLGRAAANV
                     GTLLDLRVRARGASRRVIDLELIGTGGTAHVRGGRVRSALALREQLFVIDRLHDEEGR
                     VKTFVIMGRGWGHGVGLCQVGAFGMARAGMSHEKILKAYYTDIKLTRLY"
     gene            complement(5588..>6071)
                     /locus_tag="AC159P_00490"
     CDS             complement(5588..>6071)
                     /locus_tag="AC159P_00490"
                     /inference="COORDINATES: protein motif:HMM:NF014471.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HIR3693955.1"
                     /translation="NWLTQAQAVDGLALAETTPGPLIMVLQFVGFMAAWNNAPPDMSR
                     ATSAVTGALVTTYATFLPSFVFIFLGAPYIERIRGNKNLTGALAGVTASVVGVIMNLA
                     LVFGAAVVFPRGLTGGPDIFAVTLAAAAFLFLYRFKTDVLLVIFSGGLLGLARGFLFN
                     "
BASE COUNT          924 a         1861 c         2310 g          976 t
ORIGIN      
        1 gtcggcagca ggctcgaagg gcgcgcggtg atgaggacga ggtgcgtggt ggggggcgcc
       61 tcttcgagcg tcttcaggag cgcgttcgac gccgcctcgt tcaggctgtc ggcctcggcg
      121 acgatgaaga cgcgggccgc gccctcgaag ggccggtagt tgctctcgtc gttcagatcg
      181 cgcaccgcat cgacgagcgt gctgcggttg taagggatga gctgccccac gtctctgtgg
      241 ccgctccaga tgactttcct gtgcgcgtct ttgtcgtccg aagaagggaa ctcgaagtgc
      301 gccgtgcgga cgcacgcggg gcacttgccg cagccctcga tcccgacctt gcgcacgcag
      361 ttcagcgccc gcgccagctc cagcgcgaac agctttttcc ccagcccctc ctcgcccgcg
      421 aacaaaagcg cgcccggcac gcgcccctgc ccgagcatcc gccgcagcgt ctccttcgcg
      481 cgcttgttgc cgatgagcga atcgaacata aagtgcagag gccggaggtc agaggtcgga
      541 ggtcggttcg aagagcaagt tcttaactga cctccgacct ctgacctccg gcctctatga
      601 atggaatgac gagcttcatc acctgcgcct gcgtctcctg aatcgagccg gaggcgtcga
      661 tgaggcggac gcgttcgggg tcggcggcgg cgagcttgag gtaggcgtcg cggacgcgcg
      721 tgtgaaaggc ggcgtcttcg gcgtcgagcc ggtcgtgctt cttctgcgtc gtctcgccgc
      781 gcgtgcgctt gcgtgcgcgg cggcggctct cctcgaccgt gaggtcgaag accagcgtga
      841 ggtcgggcat cagcccgccg gtggccagca cgatcagctc cgtgatgagc gcctcgggga
      901 agccgcggcc cgcgccctgg taggccgccg ttgcgtcggc gtagcggtcg gagaggacga
      961 cgtggcccga ttcgagcgcg ggccgcacga gcgtgcggac gtgctgcgcg cggtcggcgg
     1021 cgtagagcaa aagctctgcg agcggatcga cctgctcctc gttgtcgagg acgagttcgc
     1081 ggatgcgcga gccgagcgcg gtgccgcccg gctcgcgcgt cgagacgacc tcctgcccgc
     1141 gcaggcgcag ctcgctggcg agcaggcgca tctgggtgga cttcccgctc ccgtcgatcc
     1201 cctcgaaggt gataaatgcg gcggccaagc tgttctcacg ctccgtgtgt ggttgctgct
     1261 tgaagctgct tgagggtgaa ggaggcaccg gctcagtcgg cgcgaccggg ctggtcgggg
     1321 ccggcgtccg ccgccgcctc ggcgctggcg tgcgccgcgg cgcggtgctc gccggcgggg
     1381 cggcgcgtgt ggatgatggt cgagatcgcg tgcttgaaga tgagctgctc ctgcgcgccc
     1441 gattcgagca gcaccgagaa cttgtcgaag ctcttgatgc gccccgacag cttcgcgccg
     1501 ttcatcaggt agatcgtcac cgtatccttc tcgcggcgta cggtgttcaa gaacgcgtcc
     1561 tgtatgtttt ggggagtagc cttcgtgtcc actgccgtcc ctgcctgttc caagcgaaaa
     1621 agtcgttgac ggcagattac accaagacgt tcaaagttca aagttcaagg ttcgaactcg
     1681 gcgcataaag ttgcgggttc gcgactgcga aacccgactt cgggccgcgt gttccgtgct
     1741 gcgggctgcg ggccgcgaac tcaaactcgg gcgaggagcg aggcgacgtg cgcctgcacc
     1801 gcggggtctt cgccgaagcc ctcgacccac tccacgcccg gctcgcgccg gaaccatgtg
     1861 agctggcgct tggcgtagtg gcgcacgtcg agcttcgtct gctcgacggc gcttgcgaac
     1921 gagcgcgtgc cgcggaggtg ttcgacgacg cggcggtagc cgtgcgcgcc gagcgcgctc
     1981 gaagcggcgg gcacgccgcg ttcgagcagg cgccgcacct cttcgacgag gcccgcgcgg
     2041 aagtgtaact cggtgcgcgc gtcgatgcgt cggtagagtt cggcgcgcgg cgggttcagt
     2101 gcgaagaggc gcaggcgcgc ggcgtgcggc ggcggctcgg gccgcgcgcc ctgttggagg
     2161 gagagcggcg tgccggtctg gaagtagact acgagggcgc gctgcacgcg cgaccagtct
     2221 cgcgcgtgga ggcgcgcggc ggtcacgggg tcgagtcgcg cgagcatccg gtggaggtgc
     2281 tcggcgccgc gccgctcgcg cagggcggcg agcctctggc gcaagtcggc atccgtcggc
     2341 gtgctggcga agaagggccg gcgcagagct cgcaggtaga agccgctgcc gccgacgagg
     2401 acggcgaggt ggccgcgcgc ctctatctcg tcaatcgtcc tgacggcctc gcgcgcccag
     2461 tccgccgcgg tgtaggtcac gtccggcgcg acgaagtcga tgaggtggtg cgggacgccg
     2521 cgcctctccg cgagaggcac cttcgccgtc gcgatctcga tctcgcggta gacctgcacc
     2581 gagtcgcagt tgaccacctc gcccccgaag cgtagcgcga gcgcgatccc caggtcgctc
     2641 ttgcccgacg cggtcgggcc ggcgaccgcg acgacgggcc gcacggactt ttccgtttcg
     2701 ctcacgaggc aaaggaagcg agccggtaga agaccaccgc ccagatcaag agcagcacga
     2761 agagggcgcc gacgagttgc atgcgggaga agcgcatcgg attgcggatt gcggattgcg
     2821 gattagtgct cggtgatgac ggctgacgtt tgatggcggt gccgctccaa tccatcgtca
     2881 tcagtcaatc cgcaatccac aatccgcaat ccgcaatcta tcagtagagc cgcgtcagtt
     2941 tgatgtccgt gtagtacgcc ttcagaatct tttcgtgcga catgccggcg cgggccatgc
     3001 cgaaggcgcc gacctggcag aggccgacgc cgtggcccca gccgcggccc atgatgacga
     3061 aggtcttgac gcgcccctcc tcgtcgtgga ggcggtcgat gacgaagagc tgctcgcgca
     3121 aggccagcgc cgagcggacg cgaccgccgc ggacgtgcgc ggtgccgccg gtgcctatga
     3181 gttcgaggtc gatgacgcgg cgggaggcgc cgcgcgcgcg gacgcgcagg tcgaggagcg
     3241 tgccgacgtt tgccgcggcg cgaccgagtc gcgactgcgc cgcggcgggc gagagcgttt
     3301 ccgtccagtt cgagtaggcc gagaagcggt cgggcgcggc gccgttcggc gcgggttgga
     3361 cttcgaggta gctcacttcg ccgcgctggt ctgtgtggaa gacgagaggc tcgccgccga
     3421 cgaggctcac ctcgcgcacc tgatagagcg cgtcgccgaa ggcgcggaag agcagggcgc
     3481 cgcccggcat gaaggttaaa gttttgtcgc cggacttgcc cggcagacgg aagatgagag
     3541 cgccgccggc gtcaatccgc gcggtcgctt tctgcaatcg gaagagtccg cgcgcttcaa
     3601 aggcgtgcgc gacggcgcgg agcacgcgcg cgcgcgtcat cggctggcgg ggtctgagag
     3661 tcgcgtcggg aaagagcgag aggtggccgt cgcgcagcag cgcggccacg ttggcgcggt
     3721 cgcggtcggg tatctcgtcg gcgtctttga aggcgagttg ataggcaacg tcgaggtgat
     3781 tgaggagcac gtcgccgcgg ctctcgccgt cgagagcttt cgagagcgcg gtggcgaagg
     3841 cgggcggctt cgtcgcttcg ttcgggacgg agaagaagag ttggcgcgta tgcaccgcga
     3901 cgcgttcgag cagcgcggtc acttcaccga cggtcacggg cgcggccagc cactcgtcat
     3961 cgaggcgctg ggacgacatc aagccgtgga cggtgagcag cgcggcgtcg cgcgcgctcg
     4021 aagcgtgctc ggcgagcttg atgtcgaaga gtgggcgcgc ggtgcggacg gtgtgcgggg
     4081 cgaagggttc gccggactcg taggcacact cgcgcgcgcg caggtagggc gcggcgtggc
     4141 cgccgaagat gtgttcggcg tgctcggtgc gaccgccgca ggtcgaggtg taaacggcgt
     4201 tgatggcttc gcccttgtag gtcgccacgc gaccgcgcgt ctccgcgacg gcgcggtcgg
     4261 tgagcgggtg ctcggtcgcg cgcccgccgt agacctgcga gcgcgtcgtc ggcagcaggt
     4321 cgaagcccgc cgactggaag cggcgcaggt tggcgagcgc gtaggtgcgc gcggcgacgg
     4381 cctgcgcttt caaggcttcg agttcgggga agccgccggg cgaaagctcg ttcgggacga
     4441 cgccgcgcaa atagtcttcc agcggaacga cgttgacgac cgtcagcgcg ccgcgcgtgt
     4501 tggcgaagac ttcgagcctg ccgcggtaag gcttctcgtt atagcggacg gggtgctgcg
     4561 cctcgtcgtc cgaggcgaac gtgacgggcg cgcgcacgtc cgcgagcggc tgggtcgcgc
     4621 ccggcgcgta gagtacggcg ccgcgcgtcg cctcgctcac gcgcgagacg agacgcaagc
     4681 tgcccctcgt gccgttgccg gccttcgcgg tgttgatgcc gttcgcgttg tacgtgccgt
     4741 tcgcgttcgg cgttcctctg cgcgcggaag cggggttgac gaccgcgatg ttgccggggg
     4801 cggctgtctc ttcggcggcc aaacccgcga gcgcctcgtc ctcggtcggc ggcggaggcg
     4861 tcggagtgag cggcggcatg gagcgcggct cgatgcggac gcgcgccgtc tcgaagggcg
     4921 cgggcggcgc ggaggggttc gaggtgtcga gcagacgacc cgtcgtcgag atggtcacgg
     4981 agcgcgccgt cgtggtgagt ccgatgcgga cagacggctc ctgctcgatg cggtcttcga
     5041 ggtcgtcgtc gtcgcgctct ttcgcgacgc cttcgatgcg cgctctcgcg tccgtcgcgt
     5101 cgcgggcgtt cttcgtctcg gcgggtgggg ccggtgtgct ctctgtgcgc gcgcctgcgt
     5161 cggggcgggt gacgcggcgc gggcgcgtgt tctcttgcgc cgtcccgcgc ggggtgaagg
     5221 cgagcggcgc gaagaggagg agatgcgcga agacgagcgc gagcgaacgt gtcagtgtgg
     5281 aggtgcgcat tctctttgta cttcgtcagc gggaagagcc gcggggagcc gtcagcaggg
     5341 tcattctaca acagacgcgc ggcggaaggg ggaaaaggtg aaaaggggaa gaggggaaaa
     5401 gggaaaaacg tgttcgcgct ttgaagccgt tgcaagacgc ttcggcgcac actgtaacta
     5461 tcggggcagg gcaagtccgc ggcccgtcaa gatgcgactg attcgatatt gagtgaacgc
     5521 gtgagcgcgg gcagggacaa gccctgcccc tacagacatc acgcgcgttg cgtcgtgata
     5581 cgaagattca gttgaataga aatccgcggg cgaggccgag gaggccgccg gagaagatga
     5641 cgaggagcac gtctgttttg aagcggtaga ggaagaggaa ggcggcggcg gcgagtgtga
     5701 cggcgaagat gtccgggccg ccggtgagtc cgcgcggaaa gacgaccgcc gcgccgaaga
     5761 cgagcgcgag gttcatgatg acgccgacga ccgaggcggt gacgcccgcg agcgcgcccg
     5821 tcaggttctt gttgccgcgg atgcgctcga tgtagggggc gccgaggaag atgaagacga
     5881 aggaggggag gaaggtggcg taggtggtga cgagcgcgcc ggtgacggcg ctcgtggcgc
     5941 gcgacatgtc gggcggcgcg ttgttccagg cggccatgaa gccgacgaac tgcaagacca
     6001 tgatgagcgg tccgggcgtc gtctcggcga gcgcgaggcc gtccacggcc tgcgcctgcg
     6061 tgagccagtt g
//
LOCUS       DBNGOH010000023         4302 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3485917, whole genome shotgun sequence.
ACCESSION   DBNGOH010000023 DBNGOH010000000
VERSION     DBNGOH010000023.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4302)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4302)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4302
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3485917"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..403)
                     /locus_tag="AC159P_00495"
                     /pseudo
     CDS             complement(1..403)
                     /locus_tag="AC159P_00495"
                     /inference="COORDINATES: protein motif:HMM:TIGR04256.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
     gene            367..2769
                     /gene="recG"
                     /locus_tag="AC159P_00500"
     CDS             367..2769
                     /gene="recG"
                     /locus_tag="AC159P_00500"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HIR3693956.1"
                     /translation="MSSDYPLSLGTPLTTLHRIGIARFGQPTALKLALAVANVAGKRD
                     ATEATVEDLLNYLPMRYEDRSNLARISDLRDGVEASLELYVRVAGGFQVGKNRGPKAP
                     PLFIFEVTAGDPERTGKPVVVWWFVSGRQAARIVAYWRQRFTRGARFVAFGKWEWDSR
                     RNTFALRLTKPDELEMLPGTLTPPENTLIRMAEEEAARGGAGGEDQNGGGADAGSSSQ
                     QAVEDDNAAEDVEDESSVEEATDPRLAAIHVGRRVPVYRKLGEFRTKRLREIMHAVLA
                     SLDDEAFEETLPADLIERQKLSSRAAALRRIHFPSDDAPLAEYEGARSPAHLRLIFEE
                     FFWVALAIAIRRSERVKEPKGAVIEVSERMRERMLAVLPFTLTGAQERAFTRILDDMQ
                     SEVPMNRLLQGDVGSGKTAVALLSMLAAMENGYQAALMVPTEILAEQHARNVKRLLAA
                     SPYRVELLTGSLRASEKKRLHRDLAENEIHAAVGTHALIQESVSFARLGLVVIDEQHR
                     FGVLQRAALRERGFNPDVLVMTATPIPRSLAMTVYGDLDVSVIDELPPGRTPVKTVVV
                     GEDRRAGVYKGVEREVRAGRQAYIVYPLVEESEKMDLKDATRMYEHLRDRVFPHFSIG
                     LLHGRMKPAEKEETMGRFVRGEIQVLVATTVVEVGVDVPNASVMVIEHAERFGLSQLH
                     QLRGRVGRGAEQSFCVLLASDKQTHVAQARLGIMEETSDGFRIAEKDLEIRGPGEVMG
                     TRQSGVPTFRVGNLVRDLRILEEARHEADHYLTARKRTRETSRMIERVRADARFGLAS
                     VG"
     gene            2850..3425
                     /gene="rsmD"
                     /locus_tag="AC159P_00505"
     CDS             2850..3425
                     /gene="rsmD"
                     /locus_tag="AC159P_00505"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HIR3693957.1"
                     /translation="MRVIAGQYRGRNLKNPPGMQTRPTSDRLRETLFNVLAPRVEGAR
                     FLDLCAGTGAVGIEAVSRGAAFVTFVDRVHRMCALIESNLDLCGVPEEMTEVIKADAA
                     EFLKRTAKGGDWRAGQGPWDIVFFDPPYAEDYAPVLHAFAEQAAHLLGAGGVLVVEHH
                     HKNHLRDALGQLRRWRVLKQGDSALSFYESA"
     gene            3447..3854
                     /locus_tag="AC159P_00510"
     CDS             3447..3854
                     /locus_tag="AC159P_00510"
                     /inference="COORDINATES: protein motif:HMM:NF018894.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HIR3693958.1"
                     /translation="MAPPSGKERRLKPRFNATLRCTLSLPPDEGDILFPDAYLDCRTR
                     DLSESGVGLSAPSIYLGYTCVVDEGRTLRLRLDTGAGPLELEATAAHYLRLDEADDAA
                     ATYLIGLRLTEPPGDNLTRYLDFLKQLGAAEEA"
     gene            3900..4268
                     /locus_tag="AC159P_00515"
     CDS             3900..4268
                     /locus_tag="AC159P_00515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3693959.1"
                     /translation="MITFLLTGQYMDKYHGHLEGVPDGPRLLYRTRHIFILLAGLLHL
                     GLGAYFTSRLGLWRRVLQLSGSGLISVASLLFVVAFFYEPGLRDLHTPLSHWGAYAIV
                     AGTLLHLLSGAGRGAAATDV"
BASE COUNT          722 a         1535 c         1361 g          684 t
ORIGIN      
        1 cagtcgcagg taagtgagta gctgtgcttc atgcaccggg gctattactt cgacggactt
       61 cacttcaacc acgaccgcat tagccaccag catgtcgaga cgataaccgc atgcctttgt
      121 attcgaccgg caaggggcgc tctttctcga acggtattcc gcgcagtgtc agttcccggc
      181 acaggcaccc gcagtaagca gactccaaca aacccggccc gagcgtgcgg tgtaaatcca
      241 tcgcggcgct gatgatctgt tgtgtgagtt gattcgtggg gtgattcata gaccttcctt
      301 tcttagatga gggcgggagg gcatcataaa ccaaccgcgg cgcgcgttgt gctatcatcg
      361 ccgccgatgt cttccgacta ccccctctcg ctcggcacgc cattgacgac gcttcacagg
      421 atcggcatcg cccgcttcgg gcagccgacg gcgttgaagc tcgcgttggc ggtggcgaac
      481 gtcgcgggca agcgcgacgc gacggaggcg acggtcgaag acctcttgaa ctatctgccg
      541 atgcgttacg aggatcgctc gaacctcgcg cgcatcagcg acctcaggga cggcgtcgag
      601 gcttcgctcg aactctacgt gcgcgtggcg ggcggctttc aggtcgggaa gaaccgcggg
      661 ccgaaggcgc cgccgctctt catcttcgag gtcacggcgg gcgaccccga gcggacgggc
      721 aagcccgtcg tcgtgtggtg gttcgtctcg ggcaggcagg cggcgcgcat cgtcgcctac
      781 tggcggcagc gcttcacgcg cggcgcgcgc ttcgtcgcct tcggcaagtg ggagtgggac
      841 tctcgccgca acaccttcgc gctccgcctc acgaagccgg acgagctgga gatgctgccc
      901 ggcactttga cgccgccgga gaatacgctc atccgcatgg ccgaagagga agcggcgcgc
      961 ggcggcgcgg gcggcgaaga tcagaacggg ggcggtgcgg acgccggctc gtcgtcgcaa
     1021 caagcagtcg aagacgacaa cgcggcggaa gacgttgaag acgaatcatc ggtcgaagag
     1081 gcgaccgacc cgaggctcgc cgcgattcac gtcgggcggc gcgtgcccgt ctatcgcaag
     1141 ctcggcgagt tccgcacgaa gcgcctgcgc gagatcatgc acgcggtgct cgcctccctc
     1201 gacgacgaag ccttcgaaga gacgctgccc gccgatttga tcgagcgtca gaagttaagc
     1261 tctcgcgccg ccgccctgcg ccgcatccac ttcccttccg acgacgcgcc gctcgcagag
     1321 tacgagggcg cgcgcagccc cgcgcacctg cgcctcatct tcgaagagtt cttctgggtc
     1381 gcgctggcca tcgctatccg ccgcagcgag cgcgtgaaag agccgaaggg cgccgtcatc
     1441 gaggtcagcg agaggatgcg cgagcggatg ctggccgtcc tccccttcac gctcacgggg
     1501 gcgcaggagc gcgcgttcac gcgcatcctc gacgacatgc agtcggaggt gccgatgaac
     1561 cgcctgctgc aaggcgacgt gggcagcggc aagaccgccg tcgcgctgct ctcgatgctc
     1621 gccgcgatgg agaacggcta tcaggccgcg ctcatggtgc ccacggaaat cctcgccgag
     1681 cagcacgcgc gcaacgtcaa gcggctgctc gccgcctcgc cctaccgcgt cgaactgctc
     1741 accggctctt tgcgcgcgag cgagaagaag cggctgcaca gagacctcgc cgagaacgag
     1801 attcacgccg ccgtcggcac gcacgcgctg attcaggaat ccgtttcgtt cgcgcggctc
     1861 ggcctcgtcg tcatcgacga gcagcaccgc ttcggcgtgc tgcaacgcgc ggccctgcgc
     1921 gagcgcggct tcaacccgga cgtgctcgtg atgacggcga cgcccatccc gcgctctctg
     1981 gcgatgaccg tctacggcga cctcgacgtg tccgtcatcg acgagctgcc gcccggtcgc
     2041 acgccggtca agaccgtggt cgtcggcgaa gaccgccgcg cgggcgtcta caagggcgtc
     2101 gagcgcgagg tgcgcgccgg aaggcaggcg tacatcgtct acccgctcgt cgaagagtcc
     2161 gagaagatgg acttgaagga cgcgacgcgc atgtacgaac acctgcgcga ccgcgtcttc
     2221 ccgcacttct ccatcggcct cctgcacggg cggatgaagc ccgccgagaa ggaggagacg
     2281 atgggccgct tcgtccgcgg cgagatacag gtgctcgtgg cgacgaccgt ggtcgaggtc
     2341 ggcgttgacg tgccgaacgc ctcggtcatg gtcatcgaac acgccgagcg cttcggcctc
     2401 tcgcagctcc accaactgcg cggtcgcgtc ggtcgcggcg ccgaacagtc cttctgcgtc
     2461 ctgctcgcct ccgacaagca gacccacgtc gcgcaggccc gcctcggcat catggaagag
     2521 acttcggacg gcttccgcat cgccgagaaa gacctcgaga ttcgcggccc cggcgaagtc
     2581 atgggcacgc gccagtccgg cgtccccacc ttccgcgtcg gcaacctcgt ccgcgacctc
     2641 cgcatcctcg aagaggcgcg ccacgaagca gaccactacc tcaccgcccg caagcgcaca
     2701 cgcgaaacct cccgcatgat cgaacgcgtc cgcgccgacg cacgcttcgg cctcgcctcc
     2761 gtgggataag acacggcccc tgctgcctac ggcctaccgc tttctgcctc ctacttccct
     2821 accgcccgcc gctgctttat atttgaagtg tgagagtgat cgccggccaa taccgcggac
     2881 gcaacctgaa aaacccgccg ggcatgcaga cgcgcccgac ctccgaccgt ctgcgcgaga
     2941 ccctgttcaa cgtgctcgcg ccgcgcgtcg agggcgcgcg cttcctcgac ctttgcgccg
     3001 gcacgggcgc cgtcggcatc gaggcggtct cgcgcggcgc cgccttcgtc accttcgttg
     3061 accgcgtgca caggatgtgc gcgctcatcg aatcgaacct cgatctgtgc ggcgtccccg
     3121 aagagatgac cgaggtcatc aaggccgacg ccgccgagtt cctcaagcgc acggccaaag
     3181 gcggcgactg gcgcgcggga caggggccgt gggacatcgt cttcttcgac ccgccctacg
     3241 ccgaagacta cgcgcccgtg ctccacgcct tcgccgagca ggccgcgcac ctgctcggcg
     3301 caggcggcgt cctcgtcgtc gagcaccacc acaagaacca cctccgggac gcgctcggcc
     3361 agcttcgccg ctggcgcgtc ctcaagcagg gcgactcggc gctcagcttc tacgaatccg
     3421 catagagcga gcaggagaag ccaccgatgg cgccaccctc gggcaaagaa cgccgactca
     3481 agccgcgctt caacgcgacc ctgcgctgca cactctcgct ccccccggac gagggcgaca
     3541 tcctcttccc cgacgcgtac ctcgactgcc gcacccgcga cctcagcgaa tcgggcgtcg
     3601 gcctctccgc tccctccatc tacctcggct acacctgcgt cgtggacgaa ggccgcaccc
     3661 tccgcctccg actcgacacc ggcgccggcc ccctcgaact cgaagccacc gccgcacact
     3721 acctccgcct cgacgaagcc gacgacgccg ccgccaccta cctcatcggc ctccgcctca
     3781 ccgaaccgcc cggcgacaac ctgacgcggt atctcgactt cctcaaacag ctcggcgcgg
     3841 cggaggaggc gtgaaggttg gtgatgagaa atttccatct ggcattcgga gtcttgatcg
     3901 tgatcacgtt cctgctgacc gggcagtaca tggacaagta tcacgggcac ctggaggggg
     3961 tgccggacgg gccgcggctg ttgtaccgca cgaggcacat cttcatcctg ctggcgggcc
     4021 tgctccacct cggcctcggg gcctacttca caagtcggct gggtctctgg cgcagggtct
     4081 tgcagctctc cggctcgggc ctcatctcgg tcgcctccct gctgttcgtc gtcgcgttct
     4141 tttacgagcc ggggttgaga gacctgcaca cgccgctgag ccactggggc gcctacgcca
     4201 tcgtcgccgg gacgctgctc cacctgttga gcggggcggg acgaggggcg gcggcgaccg
     4261 acgtctgacg gcgcggcgag ggcgcggcgg gcgcacgacc tt
//
LOCUS       DBNGOH010000024         6328 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_736655, whole genome shotgun sequence.
ACCESSION   DBNGOH010000024 DBNGOH010000000
VERSION     DBNGOH010000024.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6328)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6328)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6328
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_736655"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1734
                     /locus_tag="AC159P_00520"
     CDS             <1..1734
                     /locus_tag="AC159P_00520"
                     /inference="COORDINATES: protein motif:HMM:NF015140.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007154 - cell communication [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Calx-beta domain-containing protein"
                     /protein_id="HIR3693960.1"
                     /translation="LVISQVYTRGGESGAAFHNDYVEIFNAGTVPVDMEGWSVHIRGF
                     AHDPTSFGRVFFPFSRHVEPGHYVLVRLGTGGPNGAALPPAEAGVIIFDELFNVSAGG
                     AEVALLPPDAPDPPRTSNGCPALPSANIADFVGYGNAACSEGGSAAPAPGLTTALFRR
                     SNGCEETDRNNADFQLLDAAPRNSSSPPAPCGPAQHLSFFNFASTGFAGSETQTSAQV
                     AVRRTGNLSEPATVDYVVVDGTASERSDYTSAAGRLRFAAGEAQKTFDVLITNDARQE
                     QIETAEVRLSNATGVGASVGLRDKAPLSIIDDDNPPPSSNPADDSLFFVRQHYHDFLG
                     RSPDTAGLVFWRNNIESCGADLQCREVKRVDTSAAFFLSIEFQRTGFLVHRLYRAALP
                     ETAARPRGLPRYHEFIRDTQELGRGVVVGQTGWEQTLEANTQLLLADFVARPEFLAAY
                     PQTLSAAEYVDALDAHAAGVIGGASVLTPQQRDALVAGLQNGTETRATVLRKVAETQV
                     FSDAEKNRAFVLMQYIGYLRRHPLDQPDLSFDGYDFWLAKLNAFNGNYVDAEMVKAFI
                     TSIEYRRRFGQ"
     gene            2031..6218
                     /locus_tag="AC159P_00525"
     CDS             2031..6218
                     /locus_tag="AC159P_00525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3693961.1"
                     /translation="MLESLRSRRRPASARRQLLSVIIAAFIILQPVLLAAAQRKGVAA
                     KLRNDTGGLNISVTTLGTPITQNFDTLATSGTTNAWADNSTIAGWYAQFAATTNPTTY
                     RADAGGSNTGAIYSWGTGTATERAFGSIGSGTTTDVYWAVQLTNNTGSTITSLDVSYI
                     GEQWRAGGCQPTPCTPAAQTAEFQYQVANAGTITDANAPTTGWLDHNPLDFTSPVPGT
                     STATALDGNAAANRTALSSTITVSVAPGQDFWLRWVDINHANNDHGLSIDDFSVTPNG
                     AAPTPTPTPTPGLTLSINDVSQNEGNSGTTAFNFTVSLSSSTHGGVTYDICSDDGTAQ
                     DGSNPGEDTDYSPICFANQSIPNGSDTAQHTVNVNGDTNGEANETFFVNVTNVTGATI
                     ADGQGLGTILNDDLTPIHDIQGNGASSPLAGQSVTTTGIVTLLRTGSNAGGGAASGFF
                     LQSPDAAVDADPNTSQGIFVFTSSVPTYTSPATPVAVGDELTVTGTVVEFNGLTEIGS
                     VTNITAIDTGNTLPTAVTLDSTILDPTAPSTQSQLEKFESMRLSAAALLGTVPNDNFF
                     DVETVLASVPRRQVFREPGIPASDPVPPDPTSGMVDPNIPIWDENPERLKLDTNGRAG
                     SPNNAYTSNVTFSNVSGPLDFAFGEYRLVADAGFTASPNMSAVPVPTPEADEFTVAGY
                     NIENFNNNATQRQKAALTIRNVLHYPDIIGAVEIFDLADLQALRDQVNNDAVAAGDPN
                     PQYEAYLVEQDGTSEDNDQDVGYLVKTARVAVTSVTQEREEETFEEPGGGTAILHDRP
                     PLVLDATVAPSGANPQRVLVVVNQLRSFIDIELVAGDGPRVREKRKKQAESIAGLLQE
                     LQTNNPGVPVISVGDYNAFQFNSGYDDLVSVLKGNPTPDDRIVVDQSPDLVNPDLFNL
                     IDELPAAERYSFNFEGTMQTLDHVLVNTAARARNTRIAIARVNADFPEAPAAVFASNA
                     SIPERNSDHDPVVSYFALGQPQPTGSVIISEFRFRGPSPSATPSLAPDNEFVEVYNNT
                     DSDITVSTLDGSAGWAIVASDGTTLLVIPNGTVIPARGHWLGVNANGYSLSDYGGTGA
                     AEGDDGWIGSAIPDDGGVALFRTANPAFFTEAERLDAAGYTTAPALYREGAGLPTGGP
                     EFDEDVAEWSFYRDFKAPGGRPRDTGDNAADFLGVSTDGAGTGLGQRLGAPGPENLSS
                     PVARNAQFPLLLLDATQPAAAPPNRVRDFTMDPMNNSPHGTLTIRRRVVNNTGQDVSR
                     LRVRIINITTFPAPAGTADLRARTSPDVMISNIMDAATCAAEEAGPPPCTVNVVGTIL
                     EEPPAQSGGGGWNSTLSIPVSLADGDSVNVQFLLGIQQTGAFRFYINIEALTGSIQNA
                     PRRLPLSPSPRKKAATR"
BASE COUNT         1089 a         2489 c         1882 g          868 t
ORIGIN      
        1 ctcgtcatca gccaggtcta cacgcgcggc ggcgagtcgg gcgcggcctt ccacaacgac
       61 tatgtcgaga ttttcaacgc cggcacggtg cccgttgaca tggaaggctg gtcggttcac
      121 atcaggggct ttgctcacga cccgacgagc ttcgggcggg tcttcttccc cttctcgcgc
      181 cacgtcgagc cgggccacta cgtgctcgtg cggctcggca ccggcggccc gaacggcgcg
      241 gcgctgccgc ccgccgaagc cggcgtcatc atcttcgacg agttgttcaa cgtcagtgcg
      301 ggcggcgccg aagtcgcgct gctgccgccc gacgcgcccg acccgccgcg cacgagcaac
      361 ggctgcccgg cgctgccttc cgccaacatc gccgacttcg tcggctacgg caacgccgcc
      421 tgctccgagg gcggctcggc ggcgcccgcg ccggggctga cgaccgcgct cttccgccgg
      481 tcgaacggct gcgaagagac cgaccgcaac aatgccgact ttcagcttct cgacgcggcc
      541 ccgcgcaaca gctcgtcgcc gcccgcgccc tgcggcccgg cgcagcacct cagcttcttc
      601 aacttcgcct cgaccgggtt cgcgggcagc gagacccaga cgagcgcgca ggtcgccgtc
      661 cgccgcacgg gcaacctgtc ggagccggcc accgtggatt acgtcgtcgt tgacggcacg
      721 gcctccgagc gcagcgacta cacgagcgcc gcgggccgcc tgcgcttcgc cgcgggcgag
      781 gcgcagaaga ccttcgacgt gctcatcacg aacgacgcca gacaggagca gatcgagacg
      841 gcggaggtgc gtctttcgaa cgcgaccggc gtcggcgcct cggtcgggct gcgcgacaag
      901 gcgccgctct cgatcataga cgacgacaac ccgccgccct cgtcgaaccc cgcggacgac
      961 tctctcttct tcgtccgcca gcactaccac gacttcttgg gccgcagccc ggacaccgcc
     1021 gggctggtct tctggagaaa caacatcgag tcgtgcggcg cagaccttca gtgccgcgag
     1081 gtcaagcgcg tcgatacgtc cgccgccttc ttcctctcca tcgagttcca gcggacgggc
     1141 ttcctcgtcc accgcctcta ccgcgccgcg ctccccgaaa cggcggcgcg cccgcgcggg
     1201 ctgccgcgct accacgagtt catccgcgac acgcaggagc tggggcgcgg cgtcgtcgtc
     1261 gggcagaccg ggtgggagca gacgctcgaa gccaacacgc aactgctgct cgcggacttc
     1321 gtcgcgcgcc ccgagttcct cgcggcctac ccgcagacgt tgagcgccgc cgagtacgtg
     1381 gacgcgctcg acgcgcacgc ggccggcgtc atcggcggcg cgagcgtgct cacgccgcaa
     1441 cagcgcgacg cgctcgtcgc cggacttcag aacggcaccg agacgcgcgc caccgtgctc
     1501 cgcaaggtcg ccgagacaca ggtcttctcc gacgccgaga agaaccgcgc cttcgtcctc
     1561 atgcagtaca tcggctacct gcgccgtcac cccctcgacc agcccgacct cagcttcgac
     1621 ggctacgact tctggctcgc caagctcaac gccttcaacg gcaactacgt tgacgccgag
     1681 atggtcaaag ccttcatcac ctccatcgaa taccgccggc gcttcggcca gtaagcagac
     1741 ggggagtaag gagaagggga gcaggggaat aagggagaag agcgaagagg cgaaaagcgc
     1801 ggaagggagg atgaggagca gcgtgcctct catcctccct cgttctctct tctcgcatca
     1861 ccctttctcc ccttctcccc ttcccccctt ctccctatct tccttcttga cgccgtgcgc
     1921 cgcatccggt aaaagcatct cacctgcact gctcccgcgc gcctccgcac aatgtagtag
     1981 atagagagag gcgccggcct cgaagtcaaa ccgagaaagg aaccgcccac gtgctcgaat
     2041 cgctccgcag ccggcgacgc cctgcgtccg cgcgccgtca attactgtcc gtcatcatcg
     2101 ccgccttcat catcctgcaa cccgtcctgc tcgccgccgc gcagcggaag ggtgtggccg
     2161 cgaagctccg caacgacacg ggcgggctga acatcagcgt cacgacgctg gggacaccca
     2221 tcacgcagaa cttcgacacg ctcgccacct ccggcacgac caacgcatgg gcggacaact
     2281 ccaccatcgc cggctggtac gcgcagttcg cggcgaccac caacccgacg acctaccgcg
     2341 cggacgcagg gggcagcaac acgggcgcga tctacagttg gggcacgggc accgccaccg
     2401 aacgggcctt cggctccatc ggctcgggca ccacgaccga cgtttactgg gccgtgcagt
     2461 tgaccaacaa cacgggcagc accatcacct cgctcgacgt tagctacatc ggcgagcagt
     2521 ggcgcgcggg cggctgccag ccgacgccct gcacgcccgc ggcgcagaca gccgagttcc
     2581 agtatcaggt cgccaacgcg ggcacgatta ccgacgccaa cgccccgacg acgggctggc
     2641 tggatcacaa cccgctcgac ttcacgagtc ccgtgcccgg cacctcgacg gccacagcgc
     2701 tcgacggcaa cgccgcggcc aaccgcaccg cgctctcctc gaccatcacg gtgagcgtcg
     2761 cgcccggtca ggacttctgg ctccgctggg tggacatcaa ccacgccaac aacgatcacg
     2821 gcctctccat cgacgacttc tccgtcacgc ccaacggcgc cgcgcccacg ccgacgccga
     2881 cgcccacgcc cggcctcacg ctctcgatca acgacgtgtc gcagaacgag ggcaactccg
     2941 gcacgaccgc cttcaacttc accgtctcgc tctcctcctc gacgcacggc ggcgtcacct
     3001 acgacatctg ctccgacgac ggcacggcgc aggacggcag caaccccggc gaggacacgg
     3061 actactcgcc catctgcttc gccaaccaat ccatccccaa cggaagcgac acggcgcagc
     3121 acaccgtcaa cgtcaacggc gacaccaacg gcgaggcgaa cgagaccttc ttcgtcaacg
     3181 tcacgaacgt cacgggcgcg accatcgccg acgggcaggg cctcggcacc attctcaacg
     3241 acgacctgac gcccatccac gacattcagg gcaacggcgc ctcgtcgccc ctcgccggcc
     3301 agagcgtcac gacgaccggc atcgtcacgc tcctgcgcac gggcagcaac gcgggcggcg
     3361 gcgcggccag cggcttcttc ctccagtcgc ccgacgccgc ggtggacgcc gaccccaaca
     3421 cctcgcaggg catcttcgtc ttcacctcct cggtgccgac ctacacctct ccggccacgc
     3481 ccgtcgccgt cggcgacgag ctgaccgtca ccggcaccgt cgtcgagttc aacgggctga
     3541 ccgagatcgg cagcgtcacc aacatcaccg ccatcgacac cggcaacacg ctcccgaccg
     3601 ccgtcaccct cgactcgacc atcctcgacc cgaccgcgcc ctccacgcag tcgcagcttg
     3661 agaagttcga gtcgatgcgc ctctccgccg ccgcgctcct cggcaccgtg ccgaacgaca
     3721 acttcttcga cgtggagacc gtgctcgcct ccgtcccgcg ccggcaggtc ttccgcgagc
     3781 cgggcatccc ggcctccgac cccgtcccgc ccgacccgac ttcgggcatg gtcgatccga
     3841 acatccccat ctgggacgag aaccccgaac gcctcaagct tgacaccaac gggcgggccg
     3901 gttcgccgaa caacgcctac acctcgaacg tcaccttctc gaacgtctcg gggccgctcg
     3961 acttcgcctt cggcgagtac cgcctcgtcg ccgacgcggg cttcaccgcc tcgcccaaca
     4021 tgagcgccgt gcccgtgccg acgcccgagg ccgacgagtt caccgtcgcc ggctacaaca
     4081 tcgagaactt caacaacaac gcgacgcagc ggcagaaggc cgcgctcacc atccgcaacg
     4141 tcctgcacta cccggacatc atcggcgccg tcgagatttt cgacctcgcc gacttgcagg
     4201 cgttgcgcga tcaggtcaac aacgacgccg tcgccgcggg cgacccgaac ccgcagtacg
     4261 aggcttacct cgtcgagcag gacggcacga gcgaggacaa cgatcaggac gtgggctacc
     4321 tcgtcaagac cgcgcgcgtc gccgtgacga gcgtcacgca ggagcgcgag gaggagacgt
     4381 tcgaagagcc gggcggcggc acggccatcc tccacgaccg cccgccgctc gtcctcgacg
     4441 cgaccgtcgc cccctcgggc gcgaacccgc aacgcgtcct cgtcgtcgtc aaccaactcc
     4501 gctcgttcat cgacatcgag ctggtcgcgg gcgacggccc gcgcgtgcgc gagaagcgga
     4561 agaagcaggc cgaaagtatc gccggcctgc tgcaagagct acagaccaac aacccgggcg
     4621 tgccggtcat ctcggtcggc gactacaacg ccttccagtt caacagcggc tacgacgacc
     4681 tcgtcagcgt gctcaagggc aacccgacgc ccgacgaccg gatcgtcgtc gatcaaagcc
     4741 ccgacctcgt caaccccgac ctctttaacc tgatcgacga actgcccgcc gccgagcgct
     4801 acagcttcaa cttcgagggc acgatgcaga cgctcgacca cgtgctcgtc aacacggcgg
     4861 cccgcgcgcg caacacgcgc atcgccatcg cgcgcgtcaa cgcggacttc cccgaggccc
     4921 cggcagccgt cttcgcctcg aacgcctcga tccccgagcg caactccgac cacgaccccg
     4981 tcgtctccta cttcgcgctc ggccagccgc agcccacagg ctccgtcatc atctcggagt
     5041 tccgcttccg cgggccgagt ccgtcggcga ccccttcgct cgcgcccgac aacgagttcg
     5101 tcgaggttta caacaacacc gactccgaca tcaccgtctc gacgctcgac gggtctgccg
     5161 ggtgggccat cgtcgcctcc gacggcacga cgctcctcgt catcccgaac ggcaccgtca
     5221 tccctgcgcg gggccactgg ctcggcgtca acgccaacgg ctacagcctc tcggactacg
     5281 ggggcacggg cgcggcggag ggcgacgacg gctggatcgg ctccgcgatc cccgacgacg
     5341 gcggcgtcgc gctcttccgc accgcgaacc ccgccttctt cacagaggcc gagcggctcg
     5401 acgccgccgg ctacacgacc gcgcccgcgc tctaccgcga gggcgcgggc ctgccgaccg
     5461 gcggccccga gttcgacgag gatgtcgccg agtggagctt ctaccgcgac ttcaaggcgc
     5521 cgggcggtcg cccgcgagac acgggcgaca acgccgcgga cttcctcggc gtgagcacgg
     5581 acggcgcggg caccggactc ggacagcgcc tcggcgcgcc ggggccggag aacctctcgt
     5641 cgcccgtcgc gcgcaacgcg cagttcccgc tcctgctgct cgacgcgacg cagcccgccg
     5701 ccgcgccgcc gaaccgcgtc cgcgacttca cgatggaccc catgaacaac tcgccgcacg
     5761 gcacgctcac catccgccgc cgcgtcgtca acaacacggg gcaggacgtg tcgcgcctgc
     5821 gcgtccgcat catcaacatc acgaccttcc cggcgcccgc gggcaccgcc gacctgcgcg
     5881 cgcgcacctc gcccgacgtg atgatctcga acatcatgga cgcggcgacc tgcgcggcgg
     5941 aggaggcggg gccgcccccg tgcaccgtca acgtcgtggg gacgatactg gaagagcctc
     6001 cggcgcagtc gggcggcggc ggctggaact cgacgctgag catcccggtc tcgctcgcgg
     6061 acggcgactc ggtcaacgtc cagttcctgc tcggcatcca gcagaccggc gccttccgct
     6121 tctacatcaa catcgaggcg ctgaccgggt ccatccagaa cgcgccgcgc cgcctgccgt
     6181 tgtcgccttc gccgcggaag aaggcggcca cgcgctgacg tgacctttgc ccgcgcggga
     6241 ggcgtgcgcc ggcgaccgcg ctcgcgcgcg ccccgcgaat cgaaatccga acgcgtacct
     6301 cgcccgccgc ttcgagaaat gcccgctc
//
LOCUS       DBNGOH010000025         4638 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3119533, whole genome shotgun sequence.
ACCESSION   DBNGOH010000025 DBNGOH010000000
VERSION     DBNGOH010000025.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4638)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4638)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4638
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3119533"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(39..380)
                     /locus_tag="AC159P_00530"
     CDS             complement(39..380)
                     /locus_tag="AC159P_00530"
                     /inference="COORDINATES: protein motif:HMM:NF044773.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3693962.1"
                     /translation="MGGGRDEKLLKNHAGAVRLSAVTDKLAGGRLSRTDWVRPDATDT
                     LEERKVPIGACPECEAEVHVDTDADKGDTVTCDECGTELEIVGIDPVELDIAEEDLDD
                     DDDLGDDDEEY"
     gene            544..1173
                     /locus_tag="AC159P_00535"
     CDS             544..1173
                     /locus_tag="AC159P_00535"
                     /inference="COORDINATES: protein motif:HMM:NF019265.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR3693963.1"
                     /translation="MAAFDNREGAFEASAVGVPEETSDEALVAAVRAGDDAAFEALFE
                     RHKRRVARVAGRFFRTREQVEDVMQESFTKAYFALPDFAGRREQSFSAWLTRIAVNAC
                     YDELRRTRRRAENSLSEIDDDERDWLETQLRATGAGSDVEATAVSRDLAHKLLARLDP
                     EERLLLTLLDAEGLSVAEIAEATGWSVSKVKVRAHRSRAHLRKLLKKYI"
     gene            1396..1830
                     /locus_tag="AC159P_00540"
     CDS             1396..1830
                     /locus_tag="AC159P_00540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3693964.1"
                     /translation="MFRRKDKHTEDERLDGLGRAFVRAASLDEEEVERIASAPFLAAR
                     LRTRIEAARAAREESGDWLAVFGVAWRAVPAMGLAAALAVGVFFYAGGGDPFPAGLFT
                     DEALLATSESAVEGVYFDDTGALTQDEVLSTIVDENERRTQR"
     gene            1827..2228
                     /locus_tag="AC159P_00545"
     CDS             1827..2228
                     /locus_tag="AC159P_00545"
                     /inference="COORDINATES: protein motif:HMM:NF025173.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0044183 - protein folding chaperone
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HIR3693965.1"
                     /translation="MTLQGKTRLKIWLVLAGVFVIGCVTGAALDGFYRTRAGNGRGDR
                     GRKHEKMFEETRRELDLNEEQSAKARAILDETREEFRALHGEMRPRFDAVRQRARARM
                     RETLTPEQQTRFDAMMARRDQKMKDREKDKR"
     gene            2319..>4638
                     /locus_tag="AC159P_00550"
     CDS             2319..>4638
                     /locus_tag="AC159P_00550"
                     /inference="COORDINATES: protein motif:HMM:NF051698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR3693966.1"
                     /translation="MQSFGKLIGKISCVALVLALAVSTAAAQQQGAGALRGQVTDELG
                     GAVIGATVTATDAQGQAKTATTNEEGAYAFTNLAPGTYIVRVIAPGFALYENAEVAVA
                     AGSRVALDIKLGVSLEAEEVTVAAEGALSTDPTNNAGAIVLRGADIESLPEDPDDLAA
                     ALQALAGPSAGPNGGQIYIDGFTGGRLPPRASIREIRINQNPFSAEYDRLGFGRIEIL
                     TRPGTDRLRGSAFFNFNDEALNSRNPFVPNRAPYQSRQFGGNLSGTLVKGRASFFVDF
                     DKRDTDNNAFINARVLASNFNVVPFSLAVLTPQRRTSFSPRLDFSINRNHTLVARYSY
                     ERSSSEDAGIGEFSLLSRAFDRSSTEHEIRLTETAILSPTVVNETRFSFETERSDSVG
                     DNSIPTIRVNDAFTGGGSQVGLSFNESKRYELQNYTTMARGTHSIKFGARLRGISITD
                     RSEQNFGGAFTFTTLEQYSQAVQGIAGFRPTQFSISGGNPLADVSQTDLGLFIQDDWR
                     VRPDFSLSAGLRYERQTNIDSNLNFAPRVAFAWSPGATAQTPAKTVIRGGFGVFYDRF
                     GENLTLQANRFNGANQQQFIVVEPLAGDTSERAQRAREVLSQPVFTLDGVTNVPTLEQ
                     LTAFALPQTTQLVADSLQAPYTLQSALTFERQLPKNFTASVQFINTRTLHLLRTRNIN
                     APLTDEAGVPLRDENSNLIRPFAGQGNIFQYESSGKLDQRQLVFGLFNRLSPKVTLFA
                     NYALNKVESDTEGAFTFPANSYDLTGEYGRSTL"
BASE COUNT          790 a         1658 c         1430 g          760 t
ORIGIN      
        1 ttctcttatc acggtttccg gttcacggtt gacggctatt agtattcctc gtcgtcgtcg
       61 ccgaggtcgt cgtcgtcgtc gaggtcttcc tcggcgatgt cgagttcgac tgggtcaatc
      121 ccgacgatct ccagttccgt cccgcattcg tcgcacgtga cggtgtcgcc tttgtccgcg
      181 tccgtgtcca cgtggacttc ggcttcgcac tcagggcatg ctccgattgg cactttcctt
      241 tcctccagcg tgtcagtggc gtcggggcgc acccagtccg ttcgggagag ccttccgcct
      301 gcaagtttgt cggtgaccgc cgacaatcta actgcccccg cgtgattttt caacagtttt
      361 tcgtcgcgcc cgccgcccat tttcgccgcg ccccgcgcgc gccgacgcac gcgggcgggt
      421 tcaggcaaaa cccgaaagtc tgataccctt gtcccgcact tccaaccccc gctagtaatg
      481 tgaagccctg tcagcgcgca cgcgcgccgc gccccggcgc gcacgcggcg ccgttcacgt
      541 cgcatggccg ctttcgacaa cagggagggc gccttcgagg cgagcgccgt cggcgtcccc
      601 gaagagactt ccgacgaggc gctcgtcgcc gcggtgcgcg cgggcgacga cgcggcgttc
      661 gaagccctct tcgagcgcca caagcggcgg gtcgcgcgcg tggcgggccg cttcttccgc
      721 acgcgcgagc aggtcgagga cgtgatgcag gagagcttca ccaaagccta cttcgcgctg
      781 ccggacttcg ccgggcggcg cgagcagtcc ttctccgcgt ggctcacgcg catcgccgtc
      841 aacgcctgct acgacgagct gcgccggacg cgccgccgcg ccgagaactc cctttcggag
      901 atcgacgacg acgagcgcga ctggctcgaa acgcagctcc gcgccacggg cgccggcagc
      961 gacgtggagg ccaccgccgt ctcgcgcgac ctcgcgcaca aactgctcgc gcgcctcgac
     1021 cccgaagaac gcctgctcct cacgctcctc gacgccgaag gtctctccgt cgccgagatc
     1081 gccgaggcca ccggctggtc ggtctccaaa gtcaaagtcc gcgcccaccg ctcgcgcgcg
     1141 cacctcagaa aactgctcaa gaagtacatt tgagagtagg cagtaggcgg taggcagtag
     1201 gcagcaaaat cagaagacac gcgtcagaag tcagaagcag ggctatgctc ggcactttac
     1261 ctctcaagcc tcttcgccct tcttgctttt gctgcctact gcctaccgcc taccgcctac
     1321 ttttttttgt aaccgccccc cctccctcaa cgtctctact tttggaaagc cttaaactcc
     1381 gaggatttcg cccttatgtt ccgcagaaaa gacaagcaca cagaggacga gcgactggac
     1441 gggctgggtc gcgcgttcgt gcgcgccgcg tcgctggatg aggaagaggt cgagcgcatc
     1501 gcctcggcgc ccttcctcgc ggctcgcctg cgcacgcgca tcgaggcggc ccgcgccgcc
     1561 cgcgaagagt cgggcgactg gctggccgtc ttcggcgtcg cgtggcgcgc ggtgcccgcg
     1621 atgggcctcg cggcggcgct cgccgtcggc gtcttcttct acgcaggggg cggcgacccc
     1681 ttccccgcgg ggctgttcac ggacgaggcg ctgctcgcca cctcggagtc ggcggtcgag
     1741 ggcgtctact tcgacgacac gggcgcgctg acgcaggacg aggtgctctc gacgattgtt
     1801 gacgagaacg aacggaggac gcagcgatga ctttgcaggg caagacgcga ctgaaaatct
     1861 ggctcgtcct cgcgggcgtg ttcgtcatcg gctgcgtgac gggcgcggcg ctcgacggct
     1921 tctaccgcac gcgcgcgggc aacggtcgcg gcgaccgcgg gcgcaagcac gagaagatgt
     1981 tcgaagagac gcggcgcgag cttgatctta acgaagagca gtcggcgaag gcgcgcgcca
     2041 tcctcgacga gacgcgcgag gagttccgcg cgctccacgg cgagatgcgc ccgcgcttcg
     2101 acgccgtccg ccagcgcgcc cgcgcccgca tgcgcgagac gctcacgccc gaacagcaga
     2161 cgcgcttcga cgcgatgatg gcgcgccgcg accagaagat gaaagaccgc gagaaagaca
     2221 aacgatgaag gcagaaggca gaaggatgaa taaacgcatc cacgttttat tcatccttct
     2281 gcactctgac ttcatacttt ttatatggtg aggaaccaat gcaatcattc ggtaagttga
     2341 tcggaaagat ttcgtgcgtc gcgctcgtgt tggcgctggc ggtctcgacc gccgcggcgc
     2401 aacagcaggg cgcgggcgcc ttgcgcgggc aggtgacgga cgaactgggc ggcgccgtga
     2461 tcggcgcgac cgtgacggcg acggacgcgc aggggcaggc gaagaccgcg acgacgaacg
     2521 aggagggcgc atacgctttc acgaacctcg cgccgggcac gtacatcgtg cgcgtcatcg
     2581 cgccgggctt cgcgctctac gagaacgcgg aggtcgcagt ggccgccggg tcgcgcgtcg
     2641 cgctcgacat caagctcggc gtctcgctcg aagcagagga ggtgacggtc gccgccgagg
     2701 gcgcgctgag caccgacccg acgaacaacg cgggcgccat cgtcctgcgc ggcgcggaca
     2761 tcgagtctct gcccgaagac ccggacgatc tggcggcggc gctgcaagct ctggccggcc
     2821 cgtccgccgg ccccaacggc gggcagattt acatcgacgg cttcacgggc gggcgtctgc
     2881 cgccgcgcgc ctcgatccgc gagattcgca tcaaccagaa cccgttctcg gcggagtacg
     2941 accggctcgg cttcggtcgc atcgagattc tgacgcgacc cggcaccgac cgcctgcgcg
     3001 gctcggcctt cttcaacttc aacgacgagg ctttgaactc gcgcaacccc ttcgtcccga
     3061 accgcgcgcc ctaccagtcg cgccagttcg gcggcaacct ctcgggcacg ctcgtcaagg
     3121 gccgcgcctc gttcttcgtc gatttcgaca agcgcgacac ggacaacaac gccttcatca
     3181 acgcgcgcgt cctcgcctcg aacttcaacg tcgtgccctt cagtctcgcg gtgctgacgc
     3241 cgcagcggcg cacgagcttc agcccgcgcc tcgacttctc catcaaccgg aaccacacgc
     3301 tcgtcgcgcg ctactcctac gagcgttcga gttccgaaga cgccggcatc ggcgagttct
     3361 cgctgctctc gcgcgccttc gaccgctcct cgaccgagca cgaaatccgc ctcaccgaga
     3421 cggcgattct cagccccacg gtcgtcaacg agacgcgctt ctcgttcgag accgagcgct
     3481 cggattcggt cggcgacaac tcgatcccga ccatccgcgt caacgacgcc ttcaccggcg
     3541 gcggctcgca ggtcggcctc tcgttcaacg agtcgaagcg ctacgagcta cagaactaca
     3601 cgacgatggc gcggggcacg cactccatca agttcggcgc gcgcctgcgc ggtatcagca
     3661 tcaccgaccg ctcggagcag aacttcggcg gcgcgttcac cttcaccacg ctcgaacagt
     3721 acagccaggc cgtgcagggc atcgcgggct tccgccccac gcagttctcc atctccggcg
     3781 gcaacccgct ggccgacgtg agccagaccg acctcggcct cttcatccag gacgactggc
     3841 gcgtccggcc cgacttctcg ctgagcgcgg gcctgcgcta cgagcggcag acgaacatcg
     3901 acagcaacct gaacttcgcg ccgcgcgtcg ccttcgcgtg gtcgccgggc gcgaccgcgc
     3961 agacgcccgc caagaccgtc atccgcggcg gcttcggcgt cttctacgac cgcttcggcg
     4021 agaacctgac gcttcaggcc aaccgcttca acggcgccaa ccagcagcag ttcatcgtcg
     4081 tcgagccgct cgccggtgac acctccgagc gcgcccagcg cgcgagagag gttctgagcc
     4141 agcccgtctt cacgctcgac ggcgtgacga acgtcccgac gctcgaacag ctcacggcct
     4201 tcgcgctgcc gcagaccacg cagctcgtcg ccgacagctt gcaggcgccc tacacgctcc
     4261 agagcgcgct caccttcgag cggcagttgc cgaaaaactt cacggcctcc gtgcagttca
     4321 tcaacacgcg cacgctccac ctcctgcgca cgcgcaacat caacgcgccc ctgacggacg
     4381 aggcgggcgt gccgctccgc gacgagaaca gtaatttgat tcgcccgttc gcagggcagg
     4441 gcaacatctt ccagtatgaa tcgtcgggca agctcgacca gcgccagctc gtcttcggcc
     4501 tcttcaaccg cctcagcccg aaggtgacgc tcttcgccaa ctacgcactg aacaaggtgg
     4561 agagcgacac cgagggcgcg ttcacgttcc ccgccaactc ctacgatctg acgggcgagt
     4621 acgggcgctc gacgctgg
//
LOCUS       DBNGOH010000026         4450 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1653247, whole genome shotgun sequence.
ACCESSION   DBNGOH010000026 DBNGOH010000000
VERSION     DBNGOH010000026.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4450)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4450)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4450
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1653247"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(72..824)
                     /locus_tag="AC159P_00555"
     CDS             complement(72..824)
                     /locus_tag="AC159P_00555"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HIR3693967.1"
                     /translation="MNTNSTLVIVPTYNERENVSGLVEQIMRFAPEADVMLVDDNSPD
                     GTADLAVELFGADARFSVMRREGARGYGRSVIDGLRRGMDAGYARVVQMDADFSHDPA
                     SIPSLLAASRKADVVIGSRYCEGGGIGNWPLRRKLLSRFANRYVATITGMHVSDATSG
                     FRCFNRRALEYLLQGQTLAEGYAFLVEAIYRVHEAGLSVAEVPIIFVDRREGQSKISR
                     KVIFESMLMPWRLRFSRYTPNDQQRLISQQDG"
     gene            complement(924..2699)
                     /locus_tag="AC159P_00560"
     CDS             complement(924..2699)
                     /locus_tag="AC159P_00560"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArnT family glycosyltransferase"
                     /protein_id="HIR3693968.1"
                     /translation="MREDENENGKRGGSALVRALRGRWAEAACVVLLALMALNLLAVV
                     RRKSLTIDETVLIPAGVYHLKDGDYRPINEHPPFAKVISAAPLVLLGAEAPPIDPSAE
                     QDYNYFLGLFDLFWQKNQANYETLTFWSRVPAVAVTLLLGVLVFVYARRLFGPRAAVF
                     AVFLYTVEPTVLAHGRVVQTDIPSAFSYLLFSLVLYEYLLAPTLRRAGLVGAAVGLAV
                     VTKFSMVVLGPVLVLVAVMLFLSASRWNLSRGQVAAQAAVIALACLLTVHAAYFFHNR
                     TPEPYDFSLTEAGLPQSVDAALRPLMPYAHAALHRVFPSDFVSGIGWQLGHNKWGHPA
                     GLLGEHRVLGWWYYFPVAFALKTTLPFLLLSLASVAWAAWMYFARGRESKYLALLVPL
                     AFFTGLVMLSKINIGVRYFLPAYMFMFVAGGALLDWLLRNFRPRGAALALAAAALCWT
                     GLEAARAFPDYMAYMNQLASERPRWYYLSDSNVEWGDDIREMALYLKARGEKRVGAAL
                     LGGQVLARYGIDGTGIFVPPGETPEETRYVAVGASFLNGSTVPGGFEGFPLDERTRVN
                     YFDEYRRRTPEKVFGNSIYLYRMKE"
     gene            complement(2902..3771)
                     /locus_tag="AC159P_00565"
     CDS             complement(2902..3771)
                     /locus_tag="AC159P_00565"
                     /inference="COORDINATES: protein motif:HMM:NF013247.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HIR3693969.1"
                     /translation="MHPRIIVNNRLVEAARARLHAVTGATLHGRGVFTTLAVHGGRPF
                     LWGAHWLRLLEHARRVGVECGVEEQEAADALARLVEANDVKEGRARLTLLAHAARAPW
                     KTEGGGSDLLMMTGDARAATGEGDTLALTVSPHRANTLSPLAGLKCVNYLEHVLAGEE
                     ARARDFDEAVVLNERGEIVSATMANLFWVKGGTVHTPALQTGALAGTTRACVMRLAAE
                     LAVPLVEGASPLHDLAEADEIFLTSSALGVSLVTTFDFHRHTVGAGSVALRLREAYRQ
                     LTLNFTKGLRTED"
     gene            complement(3902..>4450)
                     /locus_tag="AC159P_00570"
     CDS             complement(3902..>4450)
                     /locus_tag="AC159P_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015051124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HIR3693970.1"
                     /translation="RLRAELLSSAKDRAENVMIVDLLRNDLGRVCRYGSVEVEELCAL
                     QEHPTLFHLVSKVRGQMRDGVTAGDLLRAAFPCGSITGAPKIRAMQIIDEIETAPRGF
                     SMGALGYFSYDGALDLNVAIRTITFAPDRTARFNVGGGVVAESDPSAEYDESLLKAQA
                     LLRALGAELKSREPSKAVNREP"
BASE COUNT          761 a         1469 c         1520 g          700 t
ORIGIN      
        1 gaaagggaga ggggagaaaa gcattgcgtc ttctctcccc gtctcccttt ctccccttct
       61 ccccttctgc ttcatccgtc ctgctggctg atcaggcgct gctggtcgtt gggggtgtag
      121 cggctgaagc ggaggcgcca gggcatgagc atggattcga agatgacttt gcgcgagatt
      181 ttggactggc cctcgcggcg gtctacgaag atgatgggca cctcggcgac gctgaggccg
      241 gcctcgtgga cgcggtagat ggcttcgacg aggaaggcgt agccctcggc gagcgtctgc
      301 ccttgcagca ggtattcgag cgcgcggcgg ttgaagcagc ggaagccgct ggtggcgtcg
      361 ctgacgtgca tgccggtgat ggtggcgacg tagcggttgg cgaagcggct gaggagtttg
      421 cgccgcagcg gccagttgcc gatgccgccg ccctcgcagt agcgcgagcc gatgacgaca
      481 tccgccttgc ggctggccgc gagcagcgaa gggatcgagg ccgggtcgtg cgagaagtcc
      541 gcgtccatct gcacgacacg cgcgtaaccg gcgtccatgc cgcggcgcag cccgtcgatc
      601 acgctccgcc cgtagccgcg cgcgccctcg cggcgcatga ccgagaagcg cgcgtccgcg
      661 ccgaagagtt ccacggcgag gtcggcggtg ccgtccggcg agttgtcgtc cacgagcatc
      721 acgtccgcct ccggcgcgaa cctcatgatc tgctcgacca gaccggagac gttctcgcgc
      781 tcgttgtacg tgggaacgat gacaagcgtg ctgttggtgt tcatcataat gcagaggtca
      841 gaggtcggaa gtcggaagtc ggaataaaaa ctgaaacgcc ttcttattcc gacatccgac
      901 ttccgacttc cgaattctgt ccctcattcc ttcatcctgt acaaatagat cgagttgccg
      961 aagaccttct cgggcgtgcg gcggcggtac tcgtcgaagt aattgacgcg cgtccgctcg
     1021 tcgagcggga agccttcgaa gccgccgggg acggtcgagc cgttgaggaa gctggcgccg
     1081 acggcgacgt agcgcgtctc ttcgggcgtc tcgccgggcg ggacgaagat gccggtgccg
     1141 tcgatgccgt agcgcgcgag cacctgcccg ccgagcagcg ccgcgccgac ccgcttctcg
     1201 ccgcgcgcct tcaggtagag cgccatctcg cggatgtcgt cgccccactc gacgttcgag
     1261 tccgaaaggt aataccagcg cgggcgctcg gacgcaagct gattcatgta ggccatgtag
     1321 tcggggaagg cccgcgccgc ctccagcccc gtccagcaga gcgccgccgc cgcgagcgcg
     1381 agcgccgccc cgcgcggcct gaagttcctg agcagccaat ccaacaacgc cccgcccgcg
     1441 acgaacatga acatgtaggc cggcaggaaa taacgcaccc cgatgttgat cttgctcagc
     1501 atcacgaggc cggtgaagaa cgcgagcggc accaacagcg cgaggtattt actctcgcgc
     1561 ccgcgcgcga agtacatcca cgcggcccac gcgaccgaag cgagcgagag cagcaggaac
     1621 ggcagcgtcg tcttcagcgc gaaggctacg gggaagtagt accaccagcc caacaccctg
     1681 tgctcgccga gcagccccgc cgggtggccc cacttgttgt ggccgagctg ccagccgatg
     1741 ccggagacga agtccgaagg gaagacgcgg tgcagcgccg cgtgcgcgta cggcatcaag
     1801 gggcgcagcg ccgcgtccac gctctgcggc agcccggcct cggtcagaga gaagtcgtac
     1861 ggctccggcg tgcggttgtg gaagaagtat gcggcgtgga ctgtgagcag gcacgcgagc
     1921 gcgatgacgg cggcctgcgc cgcgacctgc ccgcggctga ggttccaccg cgacgccgag
     1981 aggaagagca tgaccgcgac caacacgagc acggggccga gcaccaccat cgaaaacttc
     2041 gtgacgacgg ctagccctac tgccgcgccg accagccccg cgcgcctgag cgtcggcgcg
     2101 agcaggtatt cgtagagcac gagcgagaag agcaggtacg agaaggccga ggggatgtcc
     2161 gtctgcacga cgcgcccgtg cgcgagcacg gtcggctcga ccgtgtagag gaagaccgcg
     2221 aagaccgccg cgcgcgggcc gaacagccgc cgtgcgtaga cgaagacgag gacgcccagc
     2281 agcagcgtta ccgccaccgc cgggacgcgc gaccagaacg tcagcgtctc gtagttcgct
     2341 tgattcttct gccagaacag gtcgaagagg ccgaggaagt agttgtagtc ctgctcggcg
     2401 gacgggtcta tcggcggcgc ctccgcgccg agcagcacga gcggcgcggc ggagatcact
     2461 ttggcgaacg gcgggtgctc gttgatgggc cggtagtcgc cgtccttcag gtggtagacg
     2521 cccgccggga tgagcaccgt ctcgtcgatg gtcaggctct tgcgccggac gaccgccagc
     2581 aggttcaagg ccatcaacgc cagcagcacg acgcacgccg cctcggccca acggccccgc
     2641 agcgcgcgca ccaacgccga cccgcctcgc ttcccgttct cgttctcgtc ttctctcatt
     2701 cggtcaggac gtgccctttc cgggccgctc acgaaaagcc gctattatgg attatccggc
     2761 gcgtttgcgc caccggcgga cactaaagtg ctgaggaccg aggactgagg actgagtgag
     2821 agtactgacg agtaacgttt gatgagaggc gtgctgtccg gagtcgtcaa ggttcatgcc
     2881 tctccactca gtcctcaatc ctcagtcctc agtcctcagt cctttagtga agttgagtgt
     2941 cagttggcgg taagcctcgc gcaggcgcag ggcgacgctg ccggcgccga ctgtgtggcg
     3001 gtggaagtcg aaggtggtga cgagcgagac gccgagcgcc gaggaggtga ggaagatttc
     3061 gtcggcctcc gcgaggtcgt gcaggggcga ggcgccttcg acgagcggca cggccagctc
     3121 ggcggccagc ctcatcacgc aggcgcgcgt cgtgccggcg agcgcgcccg tctggagcgc
     3181 gggcgtgtgg acggtgcccc ccttcaccca gaagaggttg gccatcgtcg cggagacgat
     3241 ctcgccgcgc tcgttcagga cgaccgcctc gtcgaagtcg cgcgcgcgcg cctcctcccc
     3301 cgccagcacg tgttcgaggt agttgacgca cttcagcccc gcgagcggcg agagcgtgtt
     3361 cgcgcggtgc ggcgagacgg tgagcgcgag cgtgtcgcct tcgcccgtgg ccgcgcgcgc
     3421 gtcgccggtc atcatcaata aatcggagcc gccgccctcc gtcttccagg gcgcgcgcgc
     3481 cgcgtgcgcg agcagcgtca gccgcgccct cccttccttc acgtcgttcg cttcgaccag
     3541 acgcgcgagc gcgtccgccg cctcctgctc ttcgacgccg cactcgacgc cgacgcgccg
     3601 cgcgtgctcc agcaggcgca gccagtgcgc gccccacagg aacggcctgc cgccgtggac
     3661 ggcgagcgtc gtgaagacgc cccgcccgtg cagcgtcgcc cccgtgacgg cgtgcagccg
     3721 cgcccgcgcg gcctcgacca gacgattgtt gacgatgatg cgcgggtgca tccgttcgtg
     3781 ctccgcggaa acgctgcggc gggattcatc gagcgccgag agcatacatg aaaggccgcg
     3841 aaccgtgaac cgtaaaccgt aaaccgtgat aagaagaaat ccgggtctta cttatcacgg
     3901 tttacggttc acggttcacg gctttcgacg gttcacggct tttcagttct gcgcccagcg
     3961 cgcggaggag ggcctgggct ttgaggagcg attcgtcgta ctccgccgag gggtcgctct
     4021 cggcgacgac gccgccgccg acgttgaagc gggccgtgcg gtcgggcgcg aaggtgatgg
     4081 tgcggatggc gacgttgagg tcgagcgcgc cgtcgtagga gaagtagccg agggcgccca
     4141 tcgagaagcc gcgcggcgcg gtctcgatct cgtcgatgat ctgcatcgcg cgtatcttgg
     4201 gggcgcccgt gatcgagccg cagggaaaag cggcgcgcag caggtcgccc gccgtcacgc
     4261 cgtcgcgcat ctggccgcgc accttcgaga cgaggtggaa gagcgtcggg tgctcctgca
     4321 acgcacacag ctcctcgacc tcgaccgagc cgtagcggca gacgcggccc agatcgttcc
     4381 gcagcagatc gacgatcatc acgttctcgg ccctgtcctt cgcgctcgac aaaagctccg
     4441 cgcgcagccg
//
LOCUS       DBNGOH010000027         5866 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1286841, whole genome shotgun sequence.
ACCESSION   DBNGOH010000027 DBNGOH010000000
VERSION     DBNGOH010000027.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5866)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5866)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5866
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1286841"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(227..610)
                     /locus_tag="AC159P_00575"
     CDS             complement(227..610)
                     /locus_tag="AC159P_00575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3693971.1"
                     /translation="MTASYTTEQRSGPRYVIQIPVRAEWDDEASGMHIVAEGETENVG
                     PMGALVHLPQLPAVGSRVQLIVPDAQGLTISAVAEVLRLERNPVQPLAALQLIDATDE
                     WRGLVWEPSAPHLQKPDDAGADWEH"
     gene            complement(741..2702)
                     /gene="acs"
                     /locus_tag="AC159P_00580"
     CDS             complement(741..2702)
                     /gene="acs"
                     /locus_tag="AC159P_00580"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012498784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetyl-CoA synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HIR3693972.1"
                     /translation="MSSNTTSIESVLHEERVFPPPQGFSEQARVKSPEEYEALRREAE
                     ESPESFWARLAESELHWFRKWDTVLKWEPPHAEWFVGGKINAAYNCLDRHLQTWRRNK
                     AAIIWEGEPGEQRTLTYAQLHREVCRFANVLKRAGIVRGDRVALYMPLVPELAIAMLA
                     CARIGATHSVIFGGFSSSALVDRINDAGCKMVVTADGGFRRGAEVRLKDAVDEALKHT
                     PTVKGCIVVRRTGSRIHMEMGRDHWWHEVVESVDDKCAAEELDSEHPLYILYTSGTTG
                     KPKGILHTTAGYLLQTHLTTQWVFDLKDEDTYWCTADIGWVTGHSYVVYGPLSNGATV
                     LMYEGAPNHPEPDRFWSIIERHRVSIFYTAPTAIRAFIKWGERWPLKHDLSSLRLLGT
                     VGEPINPEAWMWYRETIGKGRCPIVDTWWQTETGAIMISPLPGATPTKPGSATKPLPG
                     VEVAVMTKQGEEVGPNAGGYLVIKRPWPSMLRTIWGDDERYQKQYWSEIPGVYFAGDG
                     VRCDREGYYWIMGRDDDVINVSGHRLGTMEIESALVSHESVAEAAVVARPDELKGSGI
                     VAFVTLEGEHHPSDALKEVLRRHVAKEIGALARPDEIRFSDSLPKTRSGKIMRRLLRE
                     IASSGSVSGDTTTLEDFSVLEKLRTQEEE"
     gene            3046..3915
                     /locus_tag="AC159P_00585"
     CDS             3046..3915
                     /locus_tag="AC159P_00585"
                     /inference="COORDINATES: protein motif:HMM:NF013868.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HIR3693973.1"
                     /translation="MPLPTVRVFVSSTWLDLEPERAAVEQVVQRLRETEFVGMEYFGS
                     RDETTRRASLDEVDRSEVYVGIIGARYGSGITEDEIVTQGNFPRAAAAERGGLHGAFA
                     FPVMLHEEVLGVMEFFSREPRPPELELLAMMAHVGSQLGQFVERKRAEDERARLQKEV
                     IRMQEAQLAELSTPLITLTEQIVVMPLVGSIDAGRAARMIQALLRGLEQSRAPVAIID
                     ITGVTVVDTHVANALIQLAQSARLLGTEVILTGIRAGVARSLVGLGVELGGITTRRSL
                     QGGIARAFELLRR"
     gene            3963..4358
                     /locus_tag="AC159P_00590"
     CDS             3963..4358
                     /locus_tag="AC159P_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HIR3693974.1"
                     /translation="MKVFEPIPPETERAARVTVDAAVKVHKVLGPGLVESVYEACLAH
                     ELRCRGVTAETQVAFPVVYETIRLDTGLRLDLLVEKRLVVELKAVEKMNPLYEAQLLT
                     YLKITGCRLGLLINFNVALIKDGIRRMVL"
     gene            4403..5743
                     /locus_tag="AC159P_00595"
     CDS             4403..5743
                     /locus_tag="AC159P_00595"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015352702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HIR3693975.1"
                     /translation="MMMNSSFLYRVTLTSLLLVSYNVPAKTQTGAAAQTTAAPRDERL
                     WKKALELHRKSVVIDGHNDITEFMVDTGYDLGTPSVGKYHTDLARLKESGMTGQFFSV
                     YVDRKYATKKFIEESYLTEGGSARRAMDLIDVVYRAAEKYPNDISMAYSTADIRRAKK
                     QGRVGALLGIEGGHAIENSLSALRDFYRLGVRYMTLTHNNTNEWADACCDASRHGGLS
                     DFGREVVAEMNRLGMFVDISHVSDETLADVLDVTKAPVIASHSSARVFSNHKRNIPDE
                     LLKRIGQNGGVVMINFYPVFLDEKVRQAGLDRDARLKPQRDALNEQYKADPKRLEEEL
                     QKLNAANPLPNTTLSMIVDHIDHVAKVAGVDHVGLGSDFDGVPFLPEGMEDITRLPNI
                     TYELLRRGYSERDVRKILGENLMRAFAEVERVARVESRRISGEGSTRGLNAQMK"
BASE COUNT         1059 a         1949 c         1836 g         1022 t
ORIGIN      
        1 gccgccgtct cctcgtccgg cctctcctcg gcacgcaccg ggttcatcat cgcaacgctt
       61 aacagcctcc gcctgcgcct tgcgcgtctt tgaatgggga ggcaatcata tcaccagtga
      121 gcggcaaaac gattgcggat tgcggattgg cgtgatgctt gagcttgaaa cctgatgttg
      181 aaagttcagc tcctgaaaca cgaataatcc gcaatccgca atcgtctcag tgttcccagt
      241 cggcgcccgc gtcgtccggc ttttgcaggt gcggcgccga aggctcccag accagcccgc
      301 gccactcgtc ggtggcgtcg atcagttgca gcgcggcgag cggctggacg gggttgcgtt
      361 cgagccgcag cacctctgcc acggcgctga tggtgaggcc ctgcgcgtcc ggcacgatca
      421 gttgcacgcg gctgccgacc gcgggaagct gcggcaggtg gacgagcgcg cccatcggcc
      481 cgacgttctc cgtctcgccc tcggccacga tgtgcatccc cgacgcctcg tcgtcccact
      541 cggcgcgcac cggaatctgg atcacgtaac gcgggcctga gcgctgctcg gtcgtgtagc
      601 ttgctgtcat tggggcctcc tgcgaaagat tgtgtccacc gtagagtaaa agacgaaaac
      661 ggacgagttc gtggaaaccg agccatcgga tgcgagccgg aaaagcttct ccagtcatcg
      721 ccgctccgcg gcatcattgc tcactcttct tcctgcgtcc tcaatttttc gagcacggag
      781 aaatcttcga gcgtcgtggt gtcgccggag accgagccgc tggacgcgat ctcgcgcaag
      841 aggcggcgca tgatcttgcc ggagcgcgtc ttgggaagcg agtccgagaa gcgtatctcg
      901 tcggggcgcg cgagggcgcc gatctccttg gcgacgtgcc ggcgcaaaac ttccttcagc
      961 gcgtcgctcg ggtggtgctc gccttcgagc gtgacgaagg cgacgatgcc ggagcctttc
     1021 agctcgtcgg gccgcgcgac gaccgccgcc tcggcgaccg actcgtgcga gacgagcgcg
     1081 ctctcgatct ccatcgtacc caaccggtgg cccgagacgt tgatgacatc atcgtcgcgc
     1141 cccatgatcc agtagtagcc ctcccggtcg cagcgcactc cgtcgcccgc gaagtagacg
     1201 ccgggaatct ccgaccagta ctgcttttgg tagcgctcgt cgtcgcccca gatggtgcgg
     1261 agcatcgaag gccacgggcg cttgatgacg aggtagccgc ccgcgttcgg ccccacttcc
     1321 tcgccctgct tcgtcatcac cgcgacctcg acgcccggca gaggcttggt cgccgagccg
     1381 ggcttggtcg gcgtcgcgcc gggcagaggg ctaatcatga tcgcgcccgt ctcggtctgc
     1441 caccacgtat cgacgatggg gcagcggccc ttgccgatgg tctccctgta ccacatccaa
     1501 gcctccgggt tgatcggctc gcccaccgtc ccgagcagac gcaaagaaga caggtcgtgc
     1561 ttcaggggcc agcgctcgcc ccacttgatg aaggcgcgga tggcggtcgg cgccgtgtag
     1621 aagatgctga cgcggtggcg ctcgatgatc gaccagaagc ggtcaggctc cgggtggttc
     1681 ggcgcccctt cgtacatcag cacggtcgcg ccgttcgaca gcggcccgta gacgacgtaa
     1741 gagtggcccg tcacccaacc gatatccgcc gtacaccagt acgtgtcttc gtccttgagg
     1801 tcgaagaccc actgcgtcgt caggtgcgtc tgcaaaagat acccggcggt cgtgtgcagg
     1861 atgcccttcg gcttccccgt cgtgcccgac gtgtagagga tgtagagcgg atgctcgctg
     1921 tccagttcct ccgccgcgca cttgtcatcg accgactcga ccacctcgtg ccaccagtgg
     1981 tcgcgcccca tctccatgtg gatgcgcgaa cccgtccggc gcaccacgat gcagcccttc
     2041 accgtcggcg tgtgcttcag agcctcgtcc accgcgtcct tcagccttac ttcggcgccg
     2101 cgacgaaagc ccccgtccgc cgtgacgacc atcttgcagc ccgcgtcgtt gatgcggtca
     2161 actaaggccg acgaagagaa cccgccgaag atgaccgagt gcgtcgcccc tatgcgcgcg
     2221 cacgccagca tcgcgatcgc cagctccggc acgagcggca tgtacagcgc cacgcggtcg
     2281 ccgcgcacta tccctgcgcg cttcaacacg ttcgcgaacc ggcacacctc ccggtggagc
     2341 tgcgcgtagg tcagcgtccg ctgctcgccc ggctcgccct cccagatgat cgccgccttg
     2401 ttccgccgcc acgtctgcaa gtgacggtcg aggcagttgt acgccgcgtt gatcttcccc
     2461 ccgacgaacc actccgcgtg cggcggctcc cacttcagaa ccgtgtccca cttccggaac
     2521 cagtgaagct cgctttcggc caaccgcgcc cagaaacttt ccggcgactc ctcggcctcg
     2581 cgccgcagcg cctcgtactc ctccggagac ttcacgcgcg cctgctcgga gaacccctgc
     2641 ggcggcggaa acacgcgctc ctcgtggagc accgactcga tactcgtcgt gtttgaagac
     2701 atgacttctt aggccgactc cctccaaccc tctggccgac tctccttcgc gggaaaggtg
     2761 ctcgcgccgg ccacctccct cgacgacggc ccacgcgctt catgaaagac tgacctgcgc
     2821 tgcttgacga ctgacgggga cgtgcgctcc tgtgtaccag aaaccgcccc ccgcggcaag
     2881 gctcacgacc cccgccgccg ccgtggcggc aatttacatc aagcccctcc cactgtattt
     2941 tctacccgcc gccgcgggcc gctgcgccaa ccccgcaacc cccgcccacg cacacgcaac
     3001 cgacagcgcc gccgtcgcct cacggcgaga acaggagcac cgagcttgcc actccccacc
     3061 gtccgcgtct tcgtcagctc cacctggctc gacctcgaac ccgagcgcgc agccgtcgag
     3121 caggtcgtcc aacgcctccg cgaaaccgaa ttcgtcggga tggaatactt cggcagccgc
     3181 gacgagacga cccgtcgcgc ctcgctcgac gaggttgacc gcagcgaggt ctacgtcggg
     3241 atcatcggtg cgcgctacgg ctcaggcatc acggaggatg aaatcgtgac gcaggggaac
     3301 ttcccgcgcg cggcggcggc ggagcggggc ggcctgcacg gcgcgttcgc gttcccggtg
     3361 atgctgcacg aagaggtgct cggcgtgatg gagttcttca gccgcgagcc gaggccgccg
     3421 gagcttgagc tgctggcgat gatggcgcac gtcggcagcc agctcgggca gttcgtcgag
     3481 cgcaagcgcg cagaggacga gcgcgcgcgc ttgcagaaag aggtcatccg catgcaggag
     3541 gcgcaactcg ccgagctttc gacgccgctc atcactctga ccgaacagat cgtcgtcatg
     3601 ccgctcgtcg gcagcatcga cgccgggcgc gccgcgcgga tgattcaggc gctcctgcgc
     3661 ggactcgaac agagccgcgc gcccgtcgcc atcatcgaca tcacgggcgt gacggtcgtg
     3721 gacacgcacg tcgccaacgc actcatccag ctcgcgcagt cggcgcgcct gctcggcacg
     3781 gaagtgatcc tgaccggcat ccgcgcgggc gtcgcgcgca gcctcgtcgg actcggcgtc
     3841 gaactcggcg gcatcaccac gcgcaggagc ttgcagggcg gcatcgcgcg cgcgttcgag
     3901 ctgctcaggc ggtgagcatg ggtggcaaaa aagttatacc actaagacac aaagacacag
     3961 gaatgaaagt attcgagcct ataccgcctg agaccgagcg cgcggcgcga gtgacggttg
     4021 acgcggcggt caaggttcac aaagttttag ggccggggtt ggtggagtcg gtttatgagg
     4081 cgtgtctcgc gcacgaattg agatgccgcg gagtcacggc ggaaacgcaa gttgcttttc
     4141 ccgttgtcta tgaaaccatc cgccttgata ccggcctgcg tctggatttg ctggtggaga
     4201 aacggctggt ggtcgaattg aaagctgttg aaaaaatgaa cccgctctac gaggcgcagc
     4261 tattaacgta cttaaagata accggctgtc gtttggggct gctcatcaac ttcaatgtcg
     4321 cgttaatcaa agacggcatc cggcgcatgg ttctttaagt ccctcctttt atgccttcgt
     4381 gtctttgtgg taaaccttag ccatgatgat gaattcaagt ttcctgtacc gcgtcacgct
     4441 gacatctctc cttctcgtct cgtacaacgt tccggcaaag acacagactg gtgcggcggc
     4501 gcaaacgact gccgcgccgc gcgacgagcg gctgtggaag aaagctctgg agcttcaccg
     4561 gaagtcggtc gtcatcgacg gtcacaacga catcacggag ttcatggtgg acacggggta
     4621 cgacctcggc acgccgtcgg tgggcaagta ccacacagac ctcgcgcgcc tgaaagagag
     4681 cgggatgacc gggcagttct tctcggtcta cgtggacagg aagtacgcga cgaagaagtt
     4741 catcgaggag agctacctga cggagggagg ctcggcgcgg cgcgcgatgg atttgatcga
     4801 cgtggtctac cgcgcggcgg agaagtaccc gaacgacatc tcgatggcct actcgaccgc
     4861 ggacatccgc cgcgcgaaga agcagggccg ggtcggcgcg ctcctcggca tcgagggcgg
     4921 gcacgccatc gagaactcgc tctcggcgct gcgggatttc taccggctcg gcgtgcgcta
     4981 catgactctg acgcacaaca acacgaacga gtgggcggac gcgtgctgcg atgcgagccg
     5041 ccacggcggg ctgtcggact tcgggcgcga ggtcgtcgcg gagatgaacc ggctcggcat
     5101 gttcgtggac atctcgcacg tctcggacga gacgctcgcg gacgtgctcg acgtgacgaa
     5161 ggcgcccgtc atcgcgtcgc actcttcggc gcgcgtcttc tcgaaccaca agcgcaacat
     5221 cccggacgag ctgctgaagc gcatcgggca gaacggcggc gtcgtgatga tcaacttcta
     5281 cccggtcttc ctcgacgaga aggtgcggca ggcgggactc gaccgcgacg cgcgactcaa
     5341 gccgcagcgc gacgcgctga acgagcagta caaggccgac cccaagcggc tcgaagaaga
     5401 gttgcagaag ttgaacgcgg cgaacccgct gccgaacacg acgctctcca tgatcgtcga
     5461 tcacatcgac cacgtggcga aggtcgcggg cgtggatcac gtcggcctcg gctcggactt
     5521 cgacggcgtg cccttcctgc ccgaagggat ggaagacatc acgcgcctgc cgaacatcac
     5581 gtacgagctg ctgcgccgcg gctacagcga gcgcgacgtg aggaaaatcc tgggcgagaa
     5641 tctgatgcgc gccttcgcag aggtcgagcg cgtggcgcgt gtcgagagcc gccggatcag
     5701 cggggagggc agcacgcgcg gcctgaacgc gcagatgaaa tagaacgtga gaaggaatca
     5761 gccgcggatg gacgcggata aagaagaatg agaagatggc ttcatcttct tactttttct
     5821 gatccgcgtt ttatccgcgt ccatccgcgg ctgatgcttg ttgcct
//
LOCUS       DBNGOH010000028         3916 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4219742, whole genome shotgun sequence.
ACCESSION   DBNGOH010000028 DBNGOH010000000
VERSION     DBNGOH010000028.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3916)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3916)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3916
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4219742"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..254)
                     /locus_tag="AC159P_00600"
     CDS             complement(<1..254)
                     /locus_tag="AC159P_00600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3693976.1"
                     /translation="MASKTQNTEAATRKVPASAWYALAILTLVYVLNFLDRTLIYILF
                     KPIKEEMQFSDFQLALLGTTSFVIFYTLLGVPFGRMADRTS"
     gene            complement(418..819)
                     /locus_tag="AC159P_00605"
     CDS             complement(418..819)
                     /locus_tag="AC159P_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018193153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HIR3693977.1"
                     /translation="MNLKVGDAATLSKTIRDEDVRAFAELTGDHNPVHLDDEYAAGTR
                     FGRRVAHGMLAASLISAALANELPGRGTVYLSQQLQFTAPVFPGDTVTARVVVSKVRE
                     DKPVVTLDTVCTNQRGETVIRGEAVVLLTAV"
     gene            complement(915..1880)
                     /locus_tag="AC159P_00610"
     CDS             complement(915..1880)
                     /locus_tag="AC159P_00610"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR3693978.1"
                     /translation="MKTETHNARRASVALFRIALAALVVCACVVNAAAQSAGAKSAVP
                     LTVAPVARAATQGGKEVTVFGQKIHYVEAGSGPVVVLLHGLGGNTTNWAFTAPALAAK
                     YRVIVPDQIGHGQSAKPFLNYRIGTYTDFLDKFLDELKVERATLVGNSMGGWIAALYT
                     LQKPARVERLVLVDAAGFKPPPDLDLAALSGLNPSTRAGMRQLASLVFHNKQFFTSDV
                     AIDLMLTQRMSAGDGYTIQSLVESIHRGEDMLDGRLSAIKTPTLLVWGREDGLTPLAR
                     EGEKFKKEMPAAQLLVFDECGHVPQVEKAADFNAALLKFLAGAAN"
     gene            complement(2057..3874)
                     /locus_tag="AC159P_00615"
     CDS             complement(2057..3874)
                     /locus_tag="AC159P_00615"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015645 - fatty acid ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-dependent synthetase/ligase"
                     /protein_id="HIR3693979.1"
                     /translation="MRFPNIYTLFRDRVAHFRAREGEARRDVFHFRRGDRWEGVSWER
                     FDEEAHAFASALLARGLTRGASVCILMGNVTAWPVADIGTVSAGGVGCGLYPTSSAEQ
                     CRYIIDHSDAEFVLVDTATQLDKILSVRAGLPMVKAVICLDAEAARDARGAGAGVVAY
                     QDFLREGREARARVAPLLGERAEGAQPEDTAIMVYTSGTTGLPKGACLSHRYVINSVE
                     SLRATVPVHDTDTSFSYLPYCHVAERISGLYNRLYAGASAYFVDDLSKLWQYMHEVRP
                     TVFASLPRFFEKIHARCVADLEQAPAQERRVFYDALDKGREIVRLHQARRKVPAELRA
                     DYERDALPVLARVKDYFGGRVRLATSGGAPLPAEVAEFFAAAGLPILQAYGLTENVCV
                     AFNRSDNYRFGTVGPPMPGCEVRIEPDGEILVRSEMMFSGYYKEPEKTAEVMRDGWLL
                     TGDLGEMDDGGFLKITGRKKELIVLSTGKNVAPALVENMIKESHLVSHAFVYGDGRSY
                     LVALVTLNAAEAADYARAHGIEHTSVAELARDERVRAHVREIIERANRRVSSSESVKK
                     FLVLEQDFRAERDEVTPTMKLKRDVLAKNYAEELRSLYR"
BASE COUNT          636 a         1241 c         1348 g          691 t
ORIGIN      
        1 gacgtgcggt cggccatgcg cccgaacggc acgcccaaca gcgtgtagaa gatgacgaag
       61 gacgtggtgc ctaagagcgc cagttggaaa tcgctgaact gcatctcctc cttgatcggc
      121 ttgaagagga tgtagatcag cgtgcggtcg aggaagttca ggacgtagac gagcgtcagg
      181 atcgcgagcg cgtaccacgc agaggctgga accttccgtg tcgccgcctc tgtgttctgg
      241 gtcttacttg ccataactgg agcagttatc gaatgtgttc gagacgttcg tcagaactgt
      301 aggggcaggg cttgtccgcg gcccgcgagt tccgtttgat tcaacgtcga agataatgtg
      361 acggcgggca gggacaagcc ctgcccctac agtcatcacg cgcgttgtgt ggccgactca
      421 gacggcggtg agcagcacga ccgcctcgcc gcggatgacg gtctcgccgc gctggttggt
      481 gcagacggtg tcgagcgtga cgacgggctt gtcttccctc accttcgaca ccacgacgcg
      541 tgccgtcacg gtgtcgccgg ggaagaccgg cgcggtgaac tgtagctgct ggctgaggta
      601 gaccgtgccg cgacccggca gctcgttcgc cagcgccgcc gagatgagcg acgccgccag
      661 catcccgtgc gccacgcgcc gcccgaaccg cgttcccgcc gcgtactcgt cgtctaggtg
      721 gacgggatta tggtcgcccg tcaactccgc gaacgcccgc acgtcctcgt ccctgatcgt
      781 cttcgataac gtcgccgcgt cgccgacttt cagattcact ccgctgctcc tcctttaaca
      841 gtgatgagga ctgaggactg agtgcaagca tgttcttcaa actcagtcct cagtcctcag
      901 tcctcagtac ttttttaatt cgccgcgccc gcgagaaact tgaggagggc ggcgttgaag
      961 tcggcggcct tctcgacctg cgggacgtgg ccgcactcgt cgaagacgag gagctgcgcg
     1021 gcgggcatct cctttttgaa cttctcgccc tcgcgggcga gcggcgtgag gccgtcctcg
     1081 cggccccaga cgaggagcgt cggggtcttg atggcggaga gccgcccgtc gagcatgtcc
     1141 tcgccgcgat ggatggattc gacgaggctc tggatggtgt agccgtcgcc cgcagacatg
     1201 cgctgcgtga gcatgaggtc gatggccacg tcgctggtga agaactgctt gttgtggaag
     1261 accagcgagg cgagctggcg catccctgcg cgcgtcgagg ggttgaggcc ggagagggcc
     1321 gcgaggtcga ggtcgggcgg cggcttgaag ccggcggcgt ccacgagcac gagccgctcg
     1381 acgcgcgcgg gcttttgcag cgtgtagagc gcggcgatcc agccgcccat cgagttgccg
     1441 acgagcgtcg cgcgctcgac cttcagctcg tcgaggaact tgtcgaggaa gtccgtgtag
     1501 gtgccgatgc ggtagttgag gaagggcttg gccgactgcc cgtggccgat ctggtcgggc
     1561 acgatgacgc ggtatttggc ggccagcgcc ggggcggtga aggcccagtt cgtcgtgttc
     1621 ccgccgaggc cgtgcagcag cacgacgacg gggccgctcc ccgcctcgac gtaatgaatc
     1681 ttctggccga agaccgtcac ctccttgccg ccctgcgtcg ccgcccgcgc gacgggcgcg
     1741 accgtcagcg gtacggcact cttcgcgccc gcgctttggg ccgcggcgtt cacgacacac
     1801 gcgcagacga cgagcgccgc gagcgcgata cggaaaagcg cgacgctcgc gcggcgtgcg
     1861 ttgtgtgttt ctgttttcat cttgaagtcg ctcctttacc tgccgacgac gacggaaagt
     1921 ttagcgttga aaatcctgta ggggcagggc ttgtccctgc ccttcaagct cgggcggatt
     1981 caaggttgaa tgtattgcgg ctacgggcag tgacaagcca tgtccctaca atttcaacgt
     2041 gcacactgaa ctgcccttaa cggtacagac tccgcaactc ttccgcgtag ttcttcgcca
     2101 acacgtcgcg cttgagcttc atggtcggcg tgacctcgtc gcgctcggcc cggaaatcct
     2161 gttcgaggac gaggaatttt ttgaccgact cgctcgaaga gacgcggcgg ttggcgcgct
     2221 cgatgatttc ccgaacgtgc gcgcggacgc gctcgtcgcg cgccagctcc gcgacggagg
     2281 tgtgctcgat gccgtgggcg cgggcgtagt cggcggcctc cgcggcgttg agcgtgacga
     2341 gcgcgacgag gtagctgcgc ccgtcgccgt agacgaaggc gtggctgacg aggtgcgact
     2401 ccttgatcat gttctcgacg agcgcgggcg cgacgttctt ccccgtcgag agcacgatca
     2461 gctccttctt gcgcccggtg atcttgagaa agccgccgtc gtccatctcg ccgaggtcgc
     2521 cggtgagcag ccagccgtcg cgcatcacct cggcggtctt ctcgggttcc ttgtagtaac
     2581 cggaaaacat catctcactt cgaacgagga tttcgccgtc cggctcgatg cggacttcgc
     2641 agccgggcat tggcgggccg acggtgccga agcggtaatt gtctgaccgg ttgaaggcga
     2701 cgcagacgtt ctccgtcagc ccgtaggctt gcaggatggg caggcccgcg gcggcgaaga
     2761 actccgcgac ctcggcgggc agcggcgcgc cgcccgaggt cgccagcctg acgcgaccgc
     2821 cgaagtagtc cttcacgcgc gcgagcacgg gcagcgcgtc gcgctcgtag tcggctcgca
     2881 gctctgccgg cactttccgc cgcgcttgat ggaggcggac gatctcgcgc cccttgtcga
     2941 gcgcatcgta aaagacgcgg cgctcctgcg cgggcgcctg ttcgaggtcg gcgacgcagc
     3001 gcgcgtgaat cttctcgaag aagcgcggga ggctggcgaa gaccgtcggc ctgacctcgt
     3061 gcatgtactg ccagagcttc gagaggtcgt ccacgaagta ggcggaggcg cccgcgtaga
     3121 ggcggttgta gaggccggag atacgctcgg cgacgtggca gtaagggagg tacgagaaag
     3181 acgtgtcggt gtcgtggacg ggcaccgtcg cgcgcaagga ttcgacgctg ttgatgacgt
     3241 agcggtggct cagacacgcg cccttcggca atccggtcgt gcccgaagtg tagaccatga
     3301 tcgccgtgtc ttcgggctgc gcgccctccg cgcgctcgcc gagcagcggc gcgacgcgcg
     3361 cccgcgcctc gcggccctcg cgcaggaaat cctgataggc gacgacgccc gcgcccgcgc
     3421 cgcgagcatc gcgcgcggct tcggcgtcga ggcagatgac ggccttgacc atcggcagcc
     3481 cggcgcggac gctcaggatt ttgtcgagct gcgtcgcggt gtccacgagc acgaactctg
     3541 cgtcggagtg gtcgatgatg tagcggcact gctcggcgga gctggtcggg tagaggccgc
     3601 agccgacgcc gcccgcgctc accgtcccga tgtccgcgac gggccacgcc gtcacgttgc
     3661 ccatcaggat gcacacggac gcgccccgcg tcagcccgcg cgccagcagc gccgaggcga
     3721 aggcgtgcgc ctcctcgtcg aaccgctccc acgagacgcc ctcccaccgg tcgccgcgcc
     3781 ggaagtggaa cacgtcccgc cgcgcttcgc cctcgcgcgc gcggaagtgc gcgacgcggt
     3841 cgcggaagag ggtgtagatg ttgggaaagc gcataagagt gagcggtgag cagtaagcag
     3901 tgagccgtgg atgtag
//
LOCUS       DBNGOH010000029         2130 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4586770, whole genome shotgun sequence.
ACCESSION   DBNGOH010000029 DBNGOH010000000
VERSION     DBNGOH010000029.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2130)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2130)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2130
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4586770"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="AC159P_00620"
     CDS             complement(<1..215)
                     /locus_tag="AC159P_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020596696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HIR3693980.1"
                     /translation="MDALTGEHKGTPETGRLVSLDAFRGLTIAGMILVNNPGTWGAIY
                     DPLEHAAWHGWTPTDFVFPFFLFIVGVS"
     gene            complement(253..1626)
                     /gene="glmM"
                     /locus_tag="AC159P_00625"
     CDS             complement(253..1626)
                     /gene="glmM"
                     /locus_tag="AC159P_00625"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR03990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HIR3693981.1"
                     /translation="MRAIPTLKISISGVRGVVGESLTPGLLVRFAQAFGTYTGPGTIV
                     VGRDPRTSGEMVRQAVLAGLLSTGCRVVDLGVCPVPTVQLLVRERRARGGIAITASHN
                     PAEWNALKFVNAEGLFLTGPQARQLLDIYHQGEYTKVGGAEMRAVEHDAGALDVHIKA
                     VIDALGTIPSGPSGPRRRLRVVVDSCNGAGSVVAPRLVEALGAEVVGINVTPDGSFPR
                     GAEPVPENLSALCAAVREHGADIGFAQDMDADRLAVVNEQGVPVGEELTLVLAARYVL
                     GRERGPIVANLATTHALDAVAREFGCPAYRSRIGEANVAEEMRRVGAVVGGEGNGGVI
                     YPRINFARDSLVGMALVLHLLADTGRAVSELVAELPPHFMSKRKLNCRSDRIGAVLKK
                     IRTEYAHLPVDLRDGVKVTTPDGWFLVRGSNTEPIIRIVAEADTEARAARLVEEVRGH
                     VGACLDA"
     gene            complement(1700..>2130)
                     /locus_tag="AC159P_00630"
     CDS             complement(1700..>2130)
                     /locus_tag="AC159P_00630"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF020039.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HIR3693982.1"
                     /translation="LAFPNGIVLRPDGKTLLVGESHHNRILAYEVLAPGKLGKRTVFA
                     ELPTKGDGQIASEPDGMCLDEQGNLFVAHYGMRQVQVVSPAGKIIRRYPGGNLTTSNV
                     AFGGPRMDRLYVTGALGEEKTTRGGLFRLDLKGAKGLKLK"
BASE COUNT          327 a          738 c          724 g          341 t
ORIGIN      
        1 gagacgccga cgatgaagag gaagaagggg aagacgaagt cggtcggcgt ccagccgtgc
       61 cacgcggcgt gctccagcgg gtcgtagatg gcgccccacg tgccggggtt gttgacgagg
      121 atcatgcccg cgatggtcag cccgcggaac gcgtccagcg agacgagacg gcccgtctcc
      181 ggcgtcccct tgtgctctcc ggtcaacgcg tccattccgt aaacacctcc cgactcttcc
      241 agcgttatct tctcaagcgt cgaggcacgc gccgacgtgc cccctgacct cttcgacgag
      301 gcgcgcggcg cgcgcttccg tgtcggcctc cgcgacgatg cggatgatcg gctcggtgtt
      361 cgagccgcgg acgaggaacc agccgtcggg cgtcgtcacc ttgacgccgt cgcgcaggtc
      421 aacgggcagg tgcgcgtact ccgtgcgaat cttcttgagc acggcgccga tcctgtccga
      481 acggcagttg agcttccgct tcgacatgaa gtgcggcggc agctcggcga cgagttcgga
      541 gaccgcgcgc cccgtgtccg cgaggagatg cagcacgagc gccatgccga cgaggctgtc
      601 gcgcgcgaag ttgatccgcg ggtagatgac gccgccgttg ccctcgccgc cgacgaccgc
      661 gccgacgcgc cgcatctctt cggcgacgtt ggcctcgccg atgcgcgagc ggtaggcggg
      721 gcagccgaac tcgcgcgcca cggcgtcgag cgcgtgcgtc gtggcgaggt tggcgacgat
      781 cggcccgcgc tctctgccga gcacgtagcg cgcggcgagc accagcgtca actcttcacc
      841 gaccggcacg ccctgctcgt tgacgacggc gaggcgatcc gcgtccatgt cctgcgcgaa
      901 ccctatgtcc gctccgtgtt cgcgcaccgc cgcgcacagc gccgagaggt tttcgggcac
      961 aggctccgcg ccgcgcggga agctcccgtc cggcgtgacg ttgatgccga cgacctccgc
     1021 cccgagcgcc tcgacgagac gcggcgcgac cacggagcct gcgccgttgc acgaatcgac
     1081 cacgacgcgg agcctacggc gcggccccga aggccccgag ggtatcgtgc cgagcgcgtc
     1141 aatcacggcc ttgatgtgta cgtccaaagc gcccgcgtcg tgttcgacgg cgcgcatctc
     1201 ggcgccgccg actttcgtgt actcgccctg atggtagatg tcgaggagct gccgcgcctg
     1261 cggcccggtc aggaagagac cttcggcgtt gacgaacttg agcgcgttcc actcggcggg
     1321 gttgtggctg gccgtgatgg cgatgccgcc gcgcgcgcgg cgctcgcgca ccagcagttg
     1381 cacggtcggc acggggcaca cgccgagatc gaccacgcgg cagcccgtcg agagcagccc
     1441 cgccagcacc gcctgccgca ccatctcgcc cgacgtgcgc gggtcgcgcc cgacgacaat
     1501 cgtccccggc cccgtgtacg tcccgaacgc ctgcgcgaaa cgcaccagca gccccggcgt
     1561 cagcgactcg cccaccacgc cccgcacgcc ggagatgctg atcttgagtg tcggtatcgc
     1621 tcgcataaaa aagtactgag gaccgaggac tgaggactga gtgaaagaca atctgctttt
     1681 actcagtcct cagtcctcat cacttgagct tgagtccttt cgcgcccttg aggtcgaggc
     1741 ggaagaggcc gccgcgggtg gtcttctctt cgccgagggc gccagtgacg tagagtcgat
     1801 ccatgcgcgg gccgccgaag gcgacgttgc tcgtcgtcag gttgccgccc ggatagcggc
     1861 ggatgatttt gcccgcgggg ctgacgacct gcacctgccg catgccgtag tgcgcgacga
     1921 agaggttgcc ctgctcgtcg aggcacatac cgtccggctc gctcgcgatc tggccgtcgc
     1981 ctttcgtggg cagctcggcg aagacggttc gcttgccgag cttgccgggc gccagaactt
     2041 cgtaagcgag gatgcggttg tgatggctct cgccgacgag cagcgtcttg ccgtcgggcc
     2101 tgagcacgat gccgttgggg aaggccagcc
//
LOCUS       DBNGOH010000030         2651 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3487104, whole genome shotgun sequence.
ACCESSION   DBNGOH010000030 DBNGOH010000000
VERSION     DBNGOH010000030.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2651)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2651)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2651
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3487104"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..574)
                     /locus_tag="AC159P_00635"
     CDS             complement(<1..574)
                     /locus_tag="AC159P_00635"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013007227.1"
                     /note="catalyzes the reaction of glycine with
                     5,10-methylenetetrahydrofolate to form L-serine and
                     tetrahydrofolate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HIR3693983.1"
                     /translation="MSLTAAPLRTLDPEIHAAISAELSRQQSHIELIASENFTYPAVM
                     EAQGSVLTNKYAEGYPARRWYGGCEYVDRVENLALERAKKLFGAEHANVQPHSGSQAN
                     FAVYTAVLQPGDKVLGMNLSHGGHLTHGNPANFSGKLYQFVQYGVREDNALIDYDDLA
                     AIALREKPKMITVGASAYSRVIDFGRMGEIA"
     gene            707..1783
                     /locus_tag="AC159P_00640"
     CDS             707..1783
                     /locus_tag="AC159P_00640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3693984.1"
                     /translation="MENTSAVPVTIVEADKVGSVVSKLALQKTLAVGGERRRARAGDV
                     VVVQALTDSATYNQLELPSGRLAKINPGDIMVGALGRRRALKGFVGDVPETVASGDEL
                     HLLNMGGVIGECTGHHSSLSDAIRVRVLGFVYDEEMRARNIADGALALRASLPGGVAP
                     LVVVAGACMNSGKTVAATEIVRQATRAGMRVAAAKLSGVAALRDTLNMADHGAVATAS
                     FLDCGLPSTVGVGDLAPVAKAIIARLDECAPDMIVIELGDGILGGYSVDSVFEDAELR
                     AATAALVFCASDYVGAWGGIELFKRRGMKVDVVAGSVTDSQMGEDYIEGEMGVPAGNA
                     KRNGTRLFELIKSKVEGAAEKVCV"
     gene            2065..>2651
                     /locus_tag="AC159P_00645"
     CDS             2065..>2651
                     /locus_tag="AC159P_00645"
                     /inference="COORDINATES: protein motif:HMM:NF013298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HIR3693985.1"
                     /translation="MKIRVGIFGGSGYGGAELLRVLLFHPHAEVELVTANEHAGKGVG
                     EVHRNLNGLTDLSFAKAPEDFGSWGELDCVFLALPHGQALEVAPRLPPGVKAIDLSGD
                     FRLDSAEEFAAHYGREHTAQELQREFVYGLTEVNREQIKRARLISNPGCFATATLLGL
                     APLVAAGLIDGRVVVDAKTGSSGSGAKPAANTHHP"
BASE COUNT          449 a          778 c          976 g          448 t
ORIGIN      
        1 gcgcgatttc tcccatgcgc ccaaaatcga tcacgcgcga gtacgcgctc gcgccgaccg
       61 tgatcatctt cggtttctcg cgcagtgcga tggcggcgag atcgtcgtag tcgatcaatg
      121 cattgtcctc gcgcacgccg tactgcacga actggtagag cttgcccgaa aaattcgcgg
      181 gattgccgtg cgtgaggtgt ccgccatggc tcaggttcat gccgagcacc ttgtcgccgg
      241 gttgcagcac cgccgtgtag accgcgaagt tcgcctgcga tcccgaatgc ggctgcacgt
      301 tggcgtgctc ggcaccgaaa agcttcttcg cgcgctcgag ggcgagattc tcaacccgat
      361 cgacgtactc acatccacca taccagcgcc tggccggata tccctcggcg tacttgtttg
      421 tcagcacgct cccctgcgcc tccatcacgg caggataggt gaaattctcc gaggcgatca
      481 gctcgatgtg gctctgctga cgcgacaact cggcggagat ggcagcgtgg atttcaggat
      541 cgagagtgcg aagcggcgcg gcagtgaggg acattcgatt gcggattgcg gattacggat
      601 tgcggattgg cgtgcttctc aatcctgaag cgagcttcgg acaatcaact tttgttactc
      661 gaacaatccg taatccgcaa tccgcaatcc gaagggagtc agggagatgg agaacacttc
      721 agcggtgccg gtgacaatcg tcgaggcgga caaggtcggg tcggtcgtgt cgaagctggc
      781 gttgcagaag acgctggcgg tcgggggcga gcggcggcgc gcgcgcgcgg gcgacgtggt
      841 ggtggtgcag gcgctgacgg acagcgcgac gtacaaccag ctcgaactgc cttccggcag
      901 gctggcgaag atcaatccgg gcgacatcat ggtgggcgcg ttgggccgtc gccgcgcgtt
      961 gaagggcttc gtgggcgacg tgcccgaaac ggtcgcgtcg ggcgacgagc tgcacctgct
     1021 gaacatgggc ggcgtgatcg gcgagtgcac ggggcaccat tcctctttga gcgacgcgat
     1081 ccgcgtgcgc gtgctcgggt tcgtgtacga cgaggagatg cgtgcgcgca acatcgcgga
     1141 cggggcgctg gcgttgcgcg cgtctctgcc ggggggcgtg gcgccgctgg tcgtcgtggc
     1201 gggcgcgtgc atgaacagcg gcaagacggt cgcggcgacg gagatcgtgc ggcaggcgac
     1261 gcgcgcgggg atgagggtcg cggcggcgaa gctgtcgggc gtggcggcgc tgcgcgacac
     1321 gctcaacatg gccgaccacg gggccgtggc gacggcgagc ttcctcgact gcggcctgcc
     1381 ttcgacggtg ggggtcggcg acctcgcgcc cgtggcgaag gcgatcatcg cgcggctcga
     1441 cgagtgcgcg cccgacatga tcgtcatcga gctgggcgac ggcatcctcg gcggctactc
     1501 ggtcgattcg gtgttcgaag acgcggagct gcgcgcggcg acggcggcgc tcgtcttctg
     1561 cgcgtcggac tacgtgggcg cgtggggcgg catcgagctg ttcaagcggc gcgggatgaa
     1621 ggtggacgtg gtcgccggct ccgtgaccga ctcgcagatg ggcgaggact acatcgaggg
     1681 cgagatgggc gtccccgcgg gcaacgcgaa gcgcaacggc acgcgcctgt tcgagctgat
     1741 caagtcgaag gtcgaaggcg cggcggagaa ggtctgcgtt tgaggcgtcg tcaccgtcgg
     1801 gcgcgggcga gaaggatgaa gccgacgacg acgaagacga tgccgacgta gaggaacgtg
     1861 cgattactgg cgacgccgag gggcgacgaa ggccacgccg agagcgatga aggggaaacc
     1921 ggcgcgcagt gttctcatct tttgctcctt cgatgaaagg taaagtcctt tcgacgttga
     1981 cggcgggcgc cgtcggcgta gacttcggag agtgaggaac gaatcggctg ccgattataa
     2041 gcccgttggc gggcgttgac gaaaatgaaa attcgcgtgg gcatcttcgg cggttcgggc
     2101 tacggcggcg cggagctgct gcgcgtcctg ctgttccacc cgcacgccga ggtcgagttg
     2161 gtgacggcga acgagcacgc gggcaagggc gtgggcgaag tccaccgcaa tctcaacggg
     2221 ctgaccgacc tctcgttcgc gaaggcgccg gaggacttcg gctcttgggg cgaactcgac
     2281 tgcgtcttcc tcgcgctgcc gcacgggcag gctttggagg tcgcgccgcg gctaccgccc
     2341 ggtgtgaagg cgattgacct gtcgggcgac tttcgtctgg acagcgcgga ggagttcgcg
     2401 gcgcactacg ggcgcgagca cacggcgcag gagctgcaac gcgagttcgt ctacgggctg
     2461 acggaggtga accgcgagca gatcaaacgc gcgcgtctca tcagcaaccc cggatgtttt
     2521 gcaacggcga cgctgttggg cctcgcgccg ctcgtcgccg ccggtctcat cgacgggcgc
     2581 gtggtggtgg acgcgaagac cggctcttcc ggctcgggcg cgaagccggc ggcgaacacg
     2641 caccacccgc a
//
LOCUS       DBNGOH010000031         1763 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1837731, whole genome shotgun sequence.
ACCESSION   DBNGOH010000031 DBNGOH010000000
VERSION     DBNGOH010000031.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1763)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1763)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1763
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1837731"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..653
                     /locus_tag="AC159P_00650"
     CDS             <1..653
                     /locus_tag="AC159P_00650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3693986.1"
                     /translation="LTLNQNGELSGAEDLPPAYRSMLKKALSTRQVERSSQLAGLSRP
                     PSSLMGSDGQGGEFSVIEPVGKVLLGARPTFRWSPMEGATGYVVEVYDGKFNLIATSP
                     QLTGHSWAAPQSLAPGNVYAWQVKAVKDGQEVTSPRPPAPQAKFRVLDRAKANELARA
                     KRAYPSSRLTLGLLYAEAGLLDEAEQELRLLRRANPDSELARRLHGQIQALRRRSE"
     gene            complement(678..1103)
                     /locus_tag="AC159P_00655"
     CDS             complement(678..1103)
                     /locus_tag="AC159P_00655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3693987.1"
                     /translation="MPSSRPLDWAMTDDGASSEISNAAFKNVRLFITCSPSSQATASL
                     AVVLAQREVPPAVGIQHFMPKEEVAEPYLILPTARENFFGAQGAPLADWKGEWWRRRM
                     TEALRASLKLKKTAEACCLFDWMGFVVAGDGARRLTVSS"
     gene            1102..>1763
                     /locus_tag="AC159P_00660"
     CDS             1102..>1763
                     /locus_tag="AC159P_00660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3693988.1"
                     /translation="MRFLPDVEGQFRALTERADPLGFHRGGSPNPSTCRHYQAITRVD
                     GPDGTPFFIVTRSGNLPDTEYVPDDLICNDSPGETGNGHLIVFRMDSRGKNGERLRSN
                     RLRKGVHADDTAPPLEDKASIYFTVVDGSEGGLVFRDGEGTQPQKVYQHPGGMQLVGK
                     MLAIAVETPRQFGSASDCNACVLTGSEEACDRCLNYQRAPNRTQIMFFDVSNPEEPIY
                     KS"
BASE COUNT          354 a          585 c          549 g          275 t
ORIGIN      
        1 tgttgacgct gaatcaaaac ggcgagctgt cgggcgccga agacctgccg cccgcttacc
       61 ggagcatgct gaagaaagcc ctcagcaccc gacaggtcga aaggtcttca caactcgcgg
      121 gactcagccg gccacccagc tcgctcatgg gttctgacgg acaaggcggc gagttctccg
      181 tgatcgagcc ggtcgggaaa gtgctgctcg gcgcgcggcc aaccttccgc tggtcgccga
      241 tggaaggggc gacgggctac gtcgtcgagg tgtatgacgg caagttcaac ctgatcgcca
      301 ccagcccgca actcaccggc cattcgtggg cggcgcccca atcgctcgcg cccggaaacg
      361 tctatgcgtg gcaggtgaaa gccgtcaaag acgggcagga agtcacttcg ccgcgcccgc
      421 ccgcgccgca ggcgaagttc cgcgtcctcg accgggcgaa agcgaacgag ctggcgagag
      481 ccaagcgcgc ctatccttcg tcccgcctga cactgggcct gctgtacgcg gaggcggggt
      541 tgttggacga ggccgaacag gaactgcgtc tgctgcggcg agcgaaccca gactcggaac
      601 tggcccgccg cctgcacggt cagattcagg cgttacggcg ccgaagcgaa tgatcggtta
      661 gcgtcagggc tgacgagtca cgaagaaacc gtaagccgcc tcgcgccatc gcctgccacc
      721 acgaagccca tccaatcgaa cagacagcag gcttctgctg tcttcttcaa cttcaacgac
      781 gctcgcaagg cttcggtcat ccttctcctc caccactctc ccttccagtc agcgaggggc
      841 gcgccctgag cgccaaaaaa attttcccgg gcggtcggta atatcaggta cggctcggca
      901 acttcctctt tcggcatgaa gtgttgaatg ccgactgcag gaggaacctc gcgttgagct
      961 aacaccacag ccagagacgc tgtggcctga gaagaaggag agcatgtgat gaacaagcgg
     1021 acgttcttga aagccgcgtt actgatttcg ctactggcgc cgtcatccgt catcgcccaa
     1081 tcgagcgggc gggacgacgg catgcgcttt ctgcccgatg tcgagggcca gttcagggca
     1141 ctcaccgagc gcgcggaccc actcgggttc caccggggcg ggtcgccgaa cccaagcacg
     1201 tgcaggcact atcaggcgat cacgcgggtg gacggccccg acgggacgcc gttcttcatc
     1261 gtcacgcgca gcggcaacct gccggacacc gagtacgtgc cggacgacct catatgcaac
     1321 gactcgccgg gcgagaccgg caacggtcat ctcatcgtct tccgcatgga ctcgcgcggg
     1381 aagaacgggg agcgcctgcg cagcaaccgc ctgcgcaagg gcgtgcacgc cgacgacacc
     1441 gcgccgcccc tggaggacaa ggcgtccatc tacttcacgg tcgtcgacgg cagcgaaggc
     1501 ggcctcgtct tccgcgacgg cgaaggcaca cagccgcaga aggtctatca acaccccggc
     1561 ggcatgcagc tcgtcgggaa gatgctggcg attgcggtcg agacgccgcg ccagttcggt
     1621 tccgcgagcg actgtaacgc ctgtgtcctc accgggtcgg aagaggcctg cgaccgctgt
     1681 ctcaactacc agcgcgcccc gaaccgcacg cagatcatgt tcttcgacgt gagcaacccg
     1741 gaagagccga tctacaagag cca
//
LOCUS       DBNGOH010000032         5887 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2754186, whole genome shotgun sequence.
ACCESSION   DBNGOH010000032 DBNGOH010000000
VERSION     DBNGOH010000032.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5887)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5887)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5887
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2754186"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            59..631
                     /locus_tag="AC159P_00665"
     CDS             59..631
                     /locus_tag="AC159P_00665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3693989.1"
                     /translation="MPKREPPGGRLLAEKIAPPATERRLTRGRLLADLGESLACCSAT
                     VISGRAGTGKTTLAADFAGRAGRAAAWYKVEAPDADLPVFFKYLCASVARRRPGFGAQ
                     ALWMLGETLMTGDVPLLVECFVSELLCLDEPLLVVVDDLHFVYDEEWTVRSSSSRPRG
                     NSSSASRNCLPSARSGATARCAGTISAPTR"
     gene            706..1374
                     /locus_tag="AC159P_00670"
     CDS             706..1374
                     /locus_tag="AC159P_00670"
                     /inference="COORDINATES: protein motif:HMM:NF012311.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HIR3693990.1"
                     /translation="MTVAVLDTGLDATHADIAGRVVRNVKLADLLGLPLLGFGYPLNV
                     EGLPNTDLASGHGTFVGVVVAGSGARSGGKYAGVAPGARLVGLSAGDAGLLFVLAGFD
                     YLLDRGPSLGVRVVNCSFSAGTVYDANDPVNVATRMLTDAGVNVVFSAGNEGPGAGTL
                     NPYAAAPWVVSVGATDARGRLAAFSSRGCGARRSTAAWCASSWTPCAPSPEPSIRAAR
                     RTRA"
     gene            1415..2212
                     /locus_tag="AC159P_00675"
     CDS             1415..2212
                     /locus_tag="AC159P_00675"
                     /inference="COORDINATES: protein motif:HMM:NF012613.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HIR3693991.1"
                     /translation="MGPLLTVNDLGLKLYDGGGALAATVNALNLPGITGRVESAQVYT
                     PAPGAWRASVFHTLGLLGTSQPYTGTLRVARIEYAPLRDLDSLGDRERAEMRQSIRSF
                     LMLPFGEYFFPHMAATRAELAATLVRAGRVPQYLPGQSSYTDVGGAELLGFVESAQAA
                     PSGPLFPDAHAGGTFRPDEAADRLTAAIALVRAAGLGAEAESYTGPLPDIADLNLIPS
                     ESRGYVAVALARGLLDADADQHFRPHDPLTRAQLAHAVAVVLEIASE"
     gene            complement(2699..4018)
                     /locus_tag="AC159P_00680"
     CDS             complement(2699..4018)
                     /locus_tag="AC159P_00680"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019921179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HIR3693992.1"
                     /translation="MTSKLLSVAAFGLAFMALMVAGIVDARAQGGSAGYKFDFGPGRV
                     AAGYAQVSETTAYTKERGYGFEPGGRVECVDRGGRDALGGDFCTGAKPFFFSVAVPEG
                     NYRVTVTLGDARGESVNTVKAELRRLMLERVETARGKSATRTFTVNVRTPQIAGGGEV
                     KLKDREKTIETWSWDDKLTLEFNGARPAVSAVEIEKVENVPTVFLLGDSTVCDQPLEP
                     YNSWGQMLTRFFGPGAAIANHAQSGESLRSSLGARRLDKVLSVMRPGDYLFIQFGHND
                     QKQKGEGVGAFTTYKADLKRFVAGARERGGLPVLVTSMQRRTFDAAGRVTDTHGDYTA
                     AVRQVATEDKVPLIDLDAASRLFYEALGPERSKLAFKEGDGTHHNNYGSYELAKVIVE
                     GIRANRLAVARHLVRDVPPFDPRRPDPVESFRVPPSPLTGDVKPFGN"
     gene            complement(4172..>5887)
                     /locus_tag="AC159P_00685"
     CDS             complement(4172..>5887)
                     /locus_tag="AC159P_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HIR3693993.1"
                     /translation="RVRAPKLWGLRQPNLYAAVTSVEHGGKVIDSYETVLGIRTIKFD
                     AARGFLLNGEPVRMNGVCQHHDLGALGAAINTRALERQFELLKEMGVNAVRTSHNPPA
                     PELHDLADRMGLLIMDEAFDAWRRSKKKNDYHLLFDDWHEKDLRALVRRDRNHPSVVM
                     WSTGNEISEQGSAEGHKLSAALAALVREEDATRPVTAGANHLNAGYNGFQKTVDVFGY
                     NYKPQEYGKFREKNPSLPLVGSETASTVSSRGEYFFPVVEDKSKGQSDFQMSSYDLYA
                     PPWATTPDTEFKAQDEFPFVFGEFVWTGFDYLGEPTPYNADATNLLNFTDAAERARMQ
                     QELQALGKIHVPSRSSYFGIIDLAGFKKDRFYIYQARWRPDLPVAHILPHWNWPERVG
                     QVTPVHVYTSGDEAELFLNGRSLGRKTKGPRQYRLRWDDVLYEPGELRVVASKDGKRW
                     AEQVMKTTGPAARLALKADRATVKADGQDLSFVTVAVADRNGLTVPRAKNRLRFRIEG
                     PGEIVAVDNGDATSHESFQSKERDAYNGLALVVVRTVAGKPGRIRLTAQADGLTSAEL
                     RLTSR"
BASE COUNT          844 a         1972 c         2039 g         1032 t
ORIGIN      
        1 cggacgtttt gcaaaactcg ccgccggaag aaattcaaac gtggccctga aggatttgat
       61 gccgaaacgt gagcctcccg gcgggcgcct gctcgccgag aagatcgcgc cgcctgcgac
      121 ggagcgccgc ctcacgcgcg ggcggctgct cgccgatctc ggcgagagcc tggcctgctg
      181 ctcggcgacc gtcatcagcg ggcgcgcggg gacgggcaag accacgctcg ccgccgactt
      241 cgcggggcgc gcgggccgcg ccgcggcctg gtacaaggtc gaggcgccgg acgcggactt
      301 gcccgtcttc ttcaaatacc tgtgcgcgtc ggtcgcgcgg cggcggcccg gcttcggcgc
      361 gcaggcgttg tggatgctcg gcgagacgct gatgacgggc gacgtgccgc tgctcgtcga
      421 gtgcttcgtc agcgagttgc tgtgtctgga cgagccgctg ctcgtcgtcg tcgatgacct
      481 ccacttcgtc tacgacgagg agtggacggt gcgctcgtcg tcatcacggc cacgcggaaa
      541 cagctcatcg gcatctcgca attgccttcc gtccgctcga tctggggcaa ccgcacgctg
      601 cgctggaacg atcagcgcgc cgacgcgctg acgggcgtcg cgcgcgcgcg cgccgaccaa
      661 gacctgaccg gcttcaacca cggcctgccg gtgacgggcc gcggcgtgac ggtcgccgtc
      721 ctcgacacgg gactcgacgc gacgcacgcg gacatcgcgg gccgcgtcgt caggaacgtc
      781 aagctcgcgg acttgctcgg cctgccgctg ctcggcttcg gctatccgct caacgtcgag
      841 gggctgccga acacagacct cgcgagcggc cacgggactt tcgtcggcgt cgtcgtcgcg
      901 ggcagcggcg cgcgctcggg cggcaagtac gcgggcgtcg cgccgggcgc gcggctcgtc
      961 ggtttgagcg cgggcgacgc gggcctcttg ttcgtgctcg cgggcttcga ctacctgctt
     1021 gatcgcggcc cttcgctcgg cgtgcgcgtg gtcaactgta gcttctcggc gggcaccgtc
     1081 tacgacgcga acgacccggt caacgtcgcc acgcggatgc tgacggacgc gggcgtcaac
     1141 gtcgtcttct cggcgggcaa cgagggaccg ggcgcgggca ccctgaaccc ttacgcggcg
     1201 gcgccgtggg tcgtctccgt gggcgcgacg gacgcgcgcg gacggctcgc cgccttctcc
     1261 tcgcgcggct gtggcgcgcg acgctcgacc gcggcctggt gcgcttcctc ttggaccccg
     1321 tgcgcgcctt cgccggaacc gtcaatccgg gcggcacgtc ggacacgggc ctaacgattc
     1381 cctcggacgc cttccacgcc tctgtgcgcg tcgcgtgggg ccgctcctca cggtcaacga
     1441 cctcggcctg aagctgtacg acggcggcgg cgcgctcgcc gcgaccgtga acgcgctcaa
     1501 cctgccgggc atcacgggcc gcgtcgagag cgcgcaggtc tacacgcccg cgccgggcgc
     1561 ctggcgcgcg agcgtcttcc acacgctcgg gctgctcggc acgtcgcagc cgtacaccgg
     1621 caccctgcgc gtggcgcgca tcgagtacgc gccgctgcgc gacctcgact cgctcggcga
     1681 ccgagagcgc gcggagatga ggcagagcat ccgctcgttc ctcatgctgc ccttcggcga
     1741 atacttcttc ccgcacatgg cggcgacgcg cgccgagttg gccgcgacgc tcgtgcgcgc
     1801 cgggcgcgtg ccgcaatacc tgcccggcca gtcgagttac acggacgtgg gcggcgcgga
     1861 gctgctcggc ttcgtcgaga gcgcgcaggc cgcgccctcc ggcccgctct tccctgacgc
     1921 gcacgcgggc ggcacgttca gacccgacga agccgcagac cgcctgacgg cggccatcgc
     1981 gctcgtccgc gccgccggtc tcggcgccga ggcggagagc tacacggggc cgctgcccga
     2041 catcgcggac ttgaacctta tcccttcgga gtcccgcggc tacgtcgccg tcgcgctcgc
     2101 gcgcggactc ctcgacgccg acgccgacca acacttccgc ccgcacgacc cgctcacgcg
     2161 cgcgcaactg gcgcacgccg tcgcggtcgt cctcgaaatc gcttccgaat agaggccggg
     2221 aacagaggcc gagagtgacc gcagcaacgc cctccggagg ggcgtccgaa atcagggggc
     2281 gtttgtcgcc cgcgccgatt tcggacgccc ctccgaaact ttttcgcggg gcctatcttt
     2341 ttgtatttcc gttcaaggtt tcttcaaggt ctccccgcta agatttctct cgacaagggg
     2401 ggcacgaact cggacaggct tcggcgggtt cggcacagac aagcgggtat aaattcacgc
     2461 aagtttccgc agcacgtttt ccgtagtcgg gacggaggcc gcgccgcggg gatgccggga
     2521 taccggaggg cggctcacag gcagagacgg gccgctctcc ggcccggctg tttctgacag
     2581 taggtagtag gcggtaggca gtaggcagca aagattaaag ggcggagaga cttgggtcgt
     2641 gaacccacgc atctccgccc ttcttgcttt tgctgcctac tgcctactgc ctactgtttc
     2701 aatttccgaa cggcttcacg tcgccggtga gggggctggg cgggactcgg aaactctcga
     2761 cggggtcggg gcggcgcggg tcgaaggggg gcacgtccct gacgaggtgc cgtgcgacgg
     2821 cgagccggtt ggccctgatg ccttcgacga ttaccttcgc cagctcgtag ctgccgtagt
     2881 tgttgtggtg cgtgccgtcg ccctccttga aggcgagctt cgagcgctcc ggcccgagcg
     2941 cttcgtagaa gaggcggctc gcggcgtcga ggtcgatgag aggcacttta tcttccgtcg
     3001 ccacctgccg caccgccgcc gtgtagtcgc cgtgcgtgtc ggtgacacgg cccgccgcgt
     3061 cgaaggtgcg gcgctgcatc gaggtcacga gcacgggcag cccgccgcgc tctctggcgc
     3121 ccgcgacgaa gcgcttgagg tcggccttgt aggtggtgaa ggcgccgacg ccctcgccct
     3181 tctgcttctg gtcgttgtgc ccgaactgga tgaaaaggta gtcgcccggc ctcatcacgc
     3241 tcaagacctt gtcgaggcgg cgcgcgccga gggaactcct gagcgattcg cccgactgcg
     3301 cgtggttggc gattgccgcg cccggcccga agaagcgcgt gagcatctgc ccccaactgt
     3361 tgtaaggctc gagcggctgg tcgcacacgg tcgagtcgcc caagaggaag accgtcggga
     3421 cgttctcgac tttctctatc tcgacggcgc tgacggcggg gcgcgcgccg ttgaattcga
     3481 gcgtcagctt gtcgtcccac gaccacgtct cgatagtctt ctcgcggtct ttgagcttga
     3541 cctcgccgcc gcccgcgatt tgcggcgtgc ggacgttgac ggtgaacgtg cgcgtcgcgc
     3601 tcttgccccg cgcggtctcg acgcgttcga gcatcaggcg gcgcagctcg gccttgaccg
     3661 tgttgacgga ctcgccccgc gcgtcgccga gcgtgaccgt cacgcggtag ttgccctccg
     3721 gcacggcgac cgagaagaag aagggcttcg cgcccgtgca gaagtcgccg ccgagcgcat
     3781 ccctcccccc gcggtcaacg cactcgacgc gcccgcccgg ctcgaacccg tagccgcgct
     3841 ccttcgtgta cgccgtcgtc tccgacacct gcgcgtaccc cgccgccact ctgccggggc
     3901 cgaagtcgaa cttataaccc gccgacccgc cctgcgcgcg cgcgtcaaca atgcccgcga
     3961 ccatcaacgc cataaacgcc aagccgaaag cagcgacgct gagaagctta cttgtcatgt
     4021 acttttaacc tcccgaatga gtaagcagta ggcggtaggc agtaggcagt aaaagaagtg
     4081 ccggatttgg aattcgaaat tcgagattcg aaatctcaac tctcgaatct gcctttgtat
     4141 taactgcccg ctgcctactg cctactgcct tttatctgct cgtgaggcgt agctcggcgc
     4201 tcgtcaggcc gtcggcctgt gcggtgagcc tgatgcgtcc gggtttgccc gcgacggtgc
     4261 ggacgacgac gagggcgagg ccgttgtagg cgtcgcgctc tttggactgg aacgattcgt
     4321 ggctggtggc gtcgccgtta tcgacggcga cgatctcgcc cggcccttcg atgcggaagc
     4381 ggaggcggtt cttcgcgcgc gggacggtga ggccgttcct gtccgcgacg gcgacggtga
     4441 cgaaggacaa gtcctgcccg tctgctttca cggtcgcgcg gtcggccttg agcgcgaggc
     4501 gcgcggcggg gccggtcgtt ttcatcactt gctcggccca ccgcttgccg tccttggagg
     4561 cgacgacgcg cagctcgccc ggctcgtaga gaacgtcgtc ccagcgcagg cggtactggc
     4621 gcggcccctt cgtcttccga ccgagcgagc ggccattgag gaacagctcg gcctcgtcgc
     4681 cggaggtgta gacgtggacg ggcgtgactt ggccgacgcg ctcgggccag ttccagtgcg
     4741 ggaggatgtg cgccacgggc aggtcggggc gccagcgcgc ctggtagatg tagaagcgat
     4801 ccttcttgaa gccggcgagg tcgatgatgc cgaagtagga gctgcgcgag gggacgtgga
     4861 ttttgccgag cgcctgaagc tcttgctgca tgcgcgcccg ctcggcggcg tccgtgaagt
     4921 tgaggaggtt ggtggcgtct gcattgtagg gcgtcggctc gccgaggtag tcgaagcccg
     4981 tccagacgaa ctcgccgaag acgaagggga actcgtcctg cgccttgaac tccgtgtcgg
     5041 gcgtcgtcgc ccacggcggc gcgtagaggt cgtaggaact catctgaaag tcggactggc
     5101 ccttgctctt gtcttcgacg acggggaaga agtattcgcc gcgcgagctg acggtcgaag
     5161 cggtctcgct gccgacgagc gggagcgaag ggttcttctc gcggaacttg ccgtactcct
     5221 gcggcttgta gttgtagccg aacacgtcca ccgtcttctg gaagccgttg tagcccgcgt
     5281 tcaggtggtt ggccccggcg gtgaccgggc gcgtcgcgtc ttcctcgcgg actagcgccg
     5341 cgagcgcggc ggagagcttg tggccttcgg cgctgccctg ctcgctgatc tcgttgccgg
     5401 tgctccacat gacgaccgaa gggtgattgc ggtcgcggcg gacgagcgcg cgcaagtcct
     5461 tctcgtgcca gtcgtcgaac aggaggtgat agtcgttctt cttcttcgag cgccgccacg
     5521 cgtcgaacgc ctcgtccatg atgagcaggc ccatgcggtc tgcgaggtcg tgcagctcgg
     5581 gcgcgggcgg gttgtggctg gtgcggacgg cgttgacgcc catctccttc aaaagctcga
     5641 actggcgttc gagcgcgcgc gtgttgatgg ccgcgccgag cgcgccgagg tcgtgatgtt
     5701 gacagacgcc gttcatccgc accggctcgc cgttgaggag gaagccgcgc gcggcgtcga
     5761 acttgatggt gcggatgccg aggacggtct cgtaactgtc gatgaccttg ccgccgtgct
     5821 cgacgctggt gacggcggcg tagaggttgg gctgccgcag gccccacagc ttcggggcgc
     5881 ggacgcg
//
LOCUS       DBNGOH010000033         3928 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_921735, whole genome shotgun sequence.
ACCESSION   DBNGOH010000033 DBNGOH010000000
VERSION     DBNGOH010000033.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3928)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3928)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3928
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_921735"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            203..1189
                     /gene="fabD"
                     /locus_tag="AC159P_00690"
     CDS             203..1189
                     /gene="fabD"
                     /locus_tag="AC159P_00690"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011525370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HIR3693994.1"
                     /translation="MTGHLAFIVPGQGSQAPGMGRDLWEKYPAARAVFEEADEALGFA
                     LSKLCFEGPAEELQLTENTQPAILAASVAALRAMEAEGFPLPDFVAGHSLGEYSALVA
                     AGALKLRDAVRVVRRRGRFMQEAVPVGAGAMAAVLGADFETVEAVCRETALEGEVCSP
                     ANINSPGQIVIAGSAAAVERAVPLLKARGAKRAVPLKVSAPFHCALMMPAQERLAGVL
                     AEVAFDDPRVPLVTNVDAAIIRTAAEARASLVKQVSSPVRWRESVELLEREGVGLFVE
                     VGPGKVLSGLVRQTAPRVQCVNVEDRQSLTAARAALAGTPPESGEHAAGAAD"
     gene            1233..1481
                     /locus_tag="AC159P_00695"
     CDS             1233..1481
                     /locus_tag="AC159P_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HIR3693995.1"
                     /translation="MADQEIENKVKQIIIDELGVDENEVTPNARFIDDLGADSLDTVE
                     LVMRFEEEFGIEIPDEDAEKIQSVRDAYNYIDQHKQAS"
     gene            1661..2902
                     /gene="fabF"
                     /locus_tag="AC159P_00700"
     CDS             1661..2902
                     /gene="fabF"
                     /locus_tag="AC159P_00700"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HIR3693996.1"
                     /translation="MKRRVVVTGLGLVTPLAHSVADTWAGLMAGRSGVDYIKKFDTAK
                     FSVKIAAEVKDFDPLKYVEKKEARKMGAFIHYAIAASDEAMADSGLRITDDISESVGT
                     YISSGIGDFWAIEREHEKLLNEGPHRVSPFFIPSAIVNLAAGQVSIRTGAKGPNSATA
                     TACSAGAHAIGDSFKIIQRGDADVMICGGAESAITPMSVAGFAAMRALSTRNDDPVHA
                     SRPFERDRDGFVIGEGAGMMIIEELEFARARGAKIYAELVGYGMTADAFHITMPDETA
                     SGAVRVMRMAMRDAGVEPEVVGYINAHGTSTPYNDKFETLAIKKTFGEHAYKLAVSST
                     KSMTGHLLGAAGGIEAVFSILAINRGVLPPTVNYFNPDPECDLDYVPNEPREARLDYA
                     LSNSFGFGGTNAALLFKRYEE"
     gene            2996..3763
                     /locus_tag="AC159P_00705"
     CDS             2996..3763
                     /locus_tag="AC159P_00705"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HIR3693997.1"
                     /translation="MKIIVLMKQVANKDAILRINREETWIEEGDLSFEVNESDGYALE
                     EALRLKEKKGGEVVVCTMGPQRAKSVIKDALARGADRAIHIVTDGTVHTGPFATAKVL
                     AEAIQGEQADLVLTGLQSDDHGYGQTGVILAELLGLPHATIVIEVDASGAEFRVKREL
                     ESGWYQWYTMPLPSLLTIQSGISQIRYATLKGIMAAKKKEIREVQPAAEAAAQFQRIT
                     KVYFPLKTKQTQFLGDGDAKAGAAQLAEKLRNEARVI"
BASE COUNT          719 a         1216 c         1376 g          617 t
ORIGIN      
        1 acgacgacga cgaaaacaat ccgtcgctca gtctccgcct ggcgggcggc ggctccggcg
       61 acgagactta cacgccgctc gattttgagg gcgacgacgc gcggcttgtg tggaaggcgt
      121 tgtgcaggtc ggcgagcgag atcgtcggtg tgaagtatag cgagtagata aagacggtgg
      181 ctttgcgatt gacttcgccc gtatgacagg acacctggca ttcatcgtcc cgggacaggg
      241 ttcgcaggcg cccggcatgg ggcgcgacct gtgggagaag tatccggcgg cgcgcgccgt
      301 gttcgaggag gccgacgagg ctttgggctt cgcgctctcg aagttgtgtt tcgaagggcc
      361 ggcggaagag ttgcagttga cggagaacac gcagcccgcg attctggcgg cgtccgttgc
      421 ggcgctgcgc gcgatggagg ccgaagggtt tccgttgccg gacttcgtgg cgggtcacag
      481 cctcggcgaa tactcggcgc tggtggcggc gggcgcgttg aagttgcgcg acgcggtgcg
      541 cgtcgtccgc cggcgcgggc ggttcatgca ggaggcggtg cccgtgggcg cgggcgcgat
      601 ggccgcggtg ctcggggcgg atttcgagac ggtcgaggcg gtgtgccgcg agacggcgct
      661 ggaaggcgag gtgtgcagcc cggcgaacat caactcgccc ggccagatcg tcatcgccgg
      721 gagcgccgcc gcggtcgagc gcgccgtccc gctgctcaag gcgcgcgggg cgaagcgggc
      781 cgtgccgctc aaggtgagcg cgccgttcca ctgcgcgctg atgatgccgg cgcaggagcg
      841 gctggccggg gtgttggcgg aagtcgcctt cgacgacccg cgcgtgccgc tcgtgacgaa
      901 cgtggacgcg gccatcatcc gcacggcggc agaagctcgc gcttcgctcg tcaaacaggt
      961 gtcttcgccg gtgcgctggc gcgagtcggt cgagctgttg gagcgcgagg gcgtcggctt
     1021 gttcgtcgaa gtcggccccg gcaaggtgct ctcgggcttg gtcagacaga cggcgccgcg
     1081 ggtacaatgc gtgaacgtcg aagacagaca gagtttgaca gccgcccgcg ccgcgctcgc
     1141 ggggacgccg ccggagtcgg gcgagcacgc ggcgggggcg gctgattagc cttcaaagct
     1201 tttttcactt cgtcagcaga ggagcaggac taatggccga tcaagagatc gagaataagg
     1261 tgaagcagat catcatcgac gaactcggcg tggacgagaa cgaggtcacg cccaacgcgc
     1321 gctttatcga cgacctcggc gccgattcgc tcgacacggt cgagctggtg atgcgcttcg
     1381 aggaggagtt cggcatcgag attccggacg aggacgcgga gaagattcag agcgtccgcg
     1441 acgcctacaa ctacatcgac cagcacaagc aggcatcgta gcggctgggg gcctcagcgg
     1501 cgctccgggt cgcgtgcggc ggtctgaagt taggacgcgc gtgcgcgccc atgcgcgtgc
     1561 ggttgtagcg cgaaacgtgg acgggcgcgc gggctgacag tgcccgcgcg ggcagcgcgc
     1621 cgccgctttt cggtcacaca cacggggagg agtcttggat ttgaaacgtc gcgtcgtggt
     1681 aacgggtctc ggcctcgtca cgccgctcgc ccactcggtc gcggacacgt gggccgggct
     1741 gatggcgggg cgcagcgggg tggactacat caagaagttc gacaccgcga agttctccgt
     1801 caagatcgcc gccgaggtca aggatttcga cccgctcaag tacgtcgaga agaaggaggc
     1861 gcgcaagatg ggcgccttca tccactacgc catcgccgcc tcggacgagg cgatggccga
     1921 cagcgggctt cgtatcaccg acgacatctc ggagagtgtc ggcacctaca tcagcagcgg
     1981 catcggcgac ttctgggcca tcgagcgcga gcacgagaag ctgctgaacg aagggccgca
     2041 ccgcgtctcg cccttcttca tcccctcggc catcgtcaac ctcgccgccg gccaagtctc
     2101 gatccgcacc ggcgcgaagg ggccgaactc ggctaccgcc accgcctgct cggcgggcgc
     2161 gcacgccatc ggcgacagct tcaagatcat ccagcgcggc gacgccgacg tgatgatctg
     2221 cggcggggcc gaatccgcca tcacgccgat gtcggtggcg ggcttcgccg cgatgcgcgc
     2281 gctctcgacg cgcaatgacg accccgtcca cgcctcgcgc cccttcgagc gcgaccgcga
     2341 cggcttcgtc atcggcgagg gcgccgggat gatgattatc gaagagctgg aattcgcccg
     2401 cgcgcgcggc gcgaagattt acgccgagct ggtcggctac ggcatgacgg cggacgcctt
     2461 ccacatcacg atgcccgacg agacggcctc cggcgcggtg cgcgtcatgc ggatggcaat
     2521 gcgcgacgcg ggggtggagc cggaggtggt cggctacatc aacgcccacg gcacgagcac
     2581 gccctacaac gacaagttcg agaccctcgc catcaagaag accttcggcg agcacgccta
     2641 caagctcgcc gtcagctcga cgaagtcgat gaccggccac ctcctcggcg cggcgggcgg
     2701 catcgaggcc gtcttctcga tcctcgccat caaccggggc gtgctgccgc cgaccgtcaa
     2761 ctacttcaac cccgaccccg aatgcgacct cgactacgtg cccaacgagc cgcgcgaggc
     2821 gcgcttggac tacgcgctct ccaacagctt cggcttcggc ggcaccaacg cggcgctgct
     2881 cttcaagcgg tacgaagaat gacaaagagt aggcagtagg cggtcggcag taggcagttc
     2941 aaaactgttc gttctgcctg ctgcctactg ccgaccgcct actacctacc gaattatgaa
     3001 aatcatcgtg ctcatgaagc aggtcgccaa caaggacgcg attctgcgga tcaacaggga
     3061 agagacgtgg atcgaggagg gcgacctgtc gttcgaggtg aacgagtcgg acgggtacgc
     3121 gctcgaagag gccctgcggc tgaaggagaa gaagggcggc gaggtggtcg tctgcacgat
     3181 ggggccgcag cgcgcgaaga gcgtcatcaa ggacgcgctc gcgcgcggcg cagaccgcgc
     3241 catccacatc gtcaccgacg ggacggtgca caccgggccg ttcgccacgg cgaaagtttt
     3301 ggccgaggcg attcagggcg agcaggccga cctcgtcctg acgggcttgc agtcggacga
     3361 ccacggctac gggcagacgg gcgtcattct ggcggagctg ctggggctgc cgcacgcgac
     3421 catcgtcatc gaggtggacg cgtcgggcgc ggagttccgc gtcaagcgcg agctggagag
     3481 cggctggtat cagtggtaca cgatgccgct gccgtcgctc ctgaccatcc agtccggcat
     3541 cagccagatt cgctacgcga cgctgaaggg catcatggcg gcgaagaaga aagagatcag
     3601 agaggtgcag cccgctgcgg aagccgccgc gcagttccag cgcatcacga aggtctactt
     3661 cccgctcaag accaagcaga cgcagttcct cggcgacggc gacgccaagg cgggcgccgc
     3721 gcagctcgcc gagaagctgc gcaacgaagc ccgagtgatt taagagccgt gaaccgtgaa
     3781 ccgtcaaccg tgacaagaag aagacaggtt tttatcttgt cacggttgac ggttcacggt
     3841 tcacggcctt tgacggttca cgtcatttgt caaaaaaatt atgagtaacg gaatccttgt
     3901 atttgtcgag caccgcgaag gcgccgtc
//
LOCUS       DBNGOH010000034         4284 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4404187, whole genome shotgun sequence.
ACCESSION   DBNGOH010000034 DBNGOH010000000
VERSION     DBNGOH010000034.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4284)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4284)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4284
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4404187"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1465)
                     /locus_tag="AC159P_00710"
     CDS             complement(<1..1465)
                     /locus_tag="AC159P_00710"
                     /inference="COORDINATES: protein motif:HMM:NF016196.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HIR3693998.1"
                     /translation="MGVPAIELVREIIGSKWFELGMIVLVATFHGYGAAWLAVRMLFR
                     PHRPAKLFGLTVWPQGMIPRHRERLAQTIGNAVGNELVSQDTVVNALFGTGFFRRKVE
                     TFVNSYTDELLNRNYTTFVDALPRPVRAPVLDAISALQLRVADHIADVLKSEETVEAV
                     NNFIERRVDEILAQRLGDTVGEETFEQVLGFVESRFRALVNERGFETKVRAFVSERVD
                     ALAHSQATLAEVLTPDAVAVVKERVDAQVPPIVRRLTEIATNRKTRTQMGALIKREVD
                     DYYEQLSFFKKIFISRDRIHNEVDDLINKTLPRKVEEYLSGEAFEAQAEEFLNSTIDG
                     VLARPVNELVGTVAPDKLELIKDQVAGRILALVRSPELATSVSAYATDALQRLRPHSL
                     RALLEHARPDSDERLKQFLSNGLVGVLSREDTARTINAILSAQIERLLVAPIGRLADH
                     VPEDKLRRASEALTDRITAAAEERLPAAINEFDIGGIV"
     gene            complement(1575..2552)
                     /locus_tag="AC159P_00715"
     CDS             complement(1575..2552)
                     /locus_tag="AC159P_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009966032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HIR3693999.1"
                     /translation="MAGGRIILAGGSGFLGRSLAEHLTRRGREVFVLTRAPKGKRGAA
                     RPVREVAWDGRTVGEWARLLEGAEAVFNLAGRSVNCRYTRANRREIVESRVRSVGALG
                     EAILGCARPPRVLVQAASAAIYGDAGARICDESAPAGSGFSAETCVRWEAAFDALDLP
                     STRKVLLRIGFVLGRGGGALGTLARLTRAYLGGTVGGGRQYVSWLHAEDLNRIFLWAV
                     ERGEAASGTFNATGPAPVPNAEFMEALRRALGRPWSPPVPAGVVRLGAALMLTEAELA
                     LTGRRCLPERLIEDNFKFLYTSLDEALRDLFARELMHDATHTPANTTPR"
     gene            complement(2911..>4284)
                     /locus_tag="AC159P_00720"
     CDS             complement(2911..>4284)
                     /locus_tag="AC159P_00720"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3694000.1"
                     /translation="YLGCLAHASYLIGSGGEAAVVDPQRDVDEYVAEAEGRGLKIKYV
                     VETHLHADFVSGHRELAERTGAEVVFGARAGATVPHRAVKEGDELKVGDVLLRVIETP
                     GHTPEGISLVVIDTAVSREPQKVLTGDTLFIGDVGRPDLAGARGYTTEMMAEMLYDSL
                     HGKLLKLADEVEVYPAHGAGSMCGRNISKETSSTVGEQRRFNYALQPMPKEEFVRLMT
                     ADLPERPAYFQHDAEINRAGAAALAELPRPAALAPREVALLLAQGGGHVVLDVRAASD
                     FGAGHVSGALNIGLGGQFASWAGSLIPLGTPLVIVADSEEQVDEAVTRLARVGHENVL
                     GYLDGGIDAWREAGLEATTIPQISVAELREMLDDQPGLQVLDVRRPGEYTSGHAPRAA
                     SAPLGPRLAEEAAHLDPARPVAVICAGGYRSSAATSLLAPRGFRLFNVSGGTSAWVAA
                     GYPVEMQ"
BASE COUNT          629 a         1439 c         1543 g          673 t
ORIGIN      
        1 gcacgatgcc gccgatgtcg aactcgttga tggcggcggg caggcgctct tcggcggcgg
       61 cggtgatgcg gtcggtcagc gcctcgctcg cgcggcgcag tttgtcttcg ggcacgtggt
      121 cggcgagccg cccgatgggc gcgaccaaga ggcgctcgat ctgcgcgctg aggatggcgt
      181 tgatcgtgcg cgccgtgtcc tcgcgcgaga ggacgccgac cagcccgttc gagaggaact
      241 gcttgagccg ctcgtcggag tcgggacgtg cgtgttcgag cagcgcgcgt aaggaatgtg
      301 ggcgcaggcg ctggagcgcg tcggtcgcgt aggccgagac cgaggtcgcc agctcggggc
      361 tgcgcaccag cgcgaggatg cggcccgcga cctgatcctt gatcagctcc agcttgtcgg
      421 gcgcgacggt gccgaccagc tcgttgacgg ggcgcgcgag cacgccgtcg atggtcgagt
      481 tcaggaactc ctcggcctgc gcctcgaacg cctcgcccga cagatactct tccactttac
      541 gcggcaaggt cttgttgatg aggtcgtcca cctcgttgtg gatgcggtcg cgcgagatga
      601 agattttttt gaagaacgag agctgctcgt agtagtcgtc cacctcgcgc ttgatgagcg
      661 cccccatctg cgtgcgcgtc ttgcggttgg tggcgatctc cgtgaggcgg cggacgatgg
      721 gcggcacctg cgcgtccacg cgctctttga cgacggcgac ggcgtcgggc gtgagcacct
      781 cggcgagcgt cgcctgactg tgcgcgagcg cgtccacgcg ctccgagacg aaggcgcgca
      841 ccttcgtctc gaacccgcgc tcgttgacca gcgcgcggaa gcggctctcg acgaagccca
      901 ggacctgctc gaacgtctcc tcgccgaccg tgtcgccgag gcgctgcgcg aggatttcgt
      961 ccacgcgccg ctcgatgaag ttgttgacgg cctcgaccgt ctcctcgctc ttgagcacgt
     1021 cggcgatgtg gtcggcgacg cgcaactgca aggccgagat ggcgtcgagg acgggcgcgc
     1081 gcaccgggcg gggaagcgcg tccacgaagg tcgtgtagtt tctgttcagc agctcgtccg
     1141 tgtaggagtt gacgaacgtc tcgaccttgc gccggaagaa gcctgtgccg aagagcgcgt
     1201 tgacgacggt gtcctgcgag accagctcgt tcccgacggc gttgccgatg gtctgcgcga
     1261 gccgctcgcg gtggcgcggg atcatgccct gcggccagac cgtcagcccg aacagcttcg
     1321 cgggccggtg cgggcggaac agcatccgca cggccagcca cgccgccccg tacccgtgga
     1381 acgtcgccac caggacgatc atccccaact cgaaccactt gctgccgatg atttcgcgta
     1441 ccagttcgat ggcgggaact cccacgcgcg cgccgcgaag atgtgtaaat cgggggtcag
     1501 cagcgatgct aaacgcacgg ggcgaagcgc gtcaaattat tcgcgcccgc acgcggatga
     1561 agaggcgtcg aaggtcagcg gggggtggtg ttcgccggtg tgtgcgtcgc gtcgtgcatg
     1621 agttcgcggg cgaagaggtc gcgcagcgct tcgtcgaggc tggtgtagag gaacttgaag
     1681 ttgtcttcga tgagccgttc gggcaggcag cggcggcccg tgagcgcgag ttcggcttcc
     1741 gtaagcatga gcgccgcgcc gaggcggacg acccctgcgg ggacgggcgg actccacggg
     1801 cgcccgagcg cgcggcggag cgcttccatg aactccgcgt tcgggacggg cgcggggccg
     1861 gtcgcgttga aggtgccgct ggcggcctcg ccgcgctcga cggcccagag gaagatgcgg
     1921 ttcaggtctt cggcgtggag ccaactgacg tactgccgcc cgccgccgac cgtgccgccg
     1981 aggtaggcgc gcgtgagccg tgcgagcgtg ccgagcgcgc cgcctccgcg cccgaggacg
     2041 aagccgatgc ggagcagcac cttccgcgtg gagggcaggt cgagcgcgtc gaaggccgcc
     2101 tcccagcgca cgcaggtctc tgcggagaag ccgctcccgg cgggcgcgct ctcgtcgcag
     2161 atgcgcgcgc ccgcgtcgcc gtagatggcc gccgacgccg cctgcaccag cacccgcggc
     2221 ggtcgcgcgc aaccgagaat cgcctcgccc agcgcgccca ccgagcggac gcgcgactcg
     2281 acgatctcgc ggcggttcgc gcgcgtgtag cggcagttca cgctccggcc cgcgaggttg
     2341 aagacggcct ccgcgccttc cagcagccgc gcccactcgc cgaccgtccg cccgtcccac
     2401 gccacctcgc gcacagggcg cgcagccccg cgcttcccct tcggcgcgcg tgtcagcacg
     2461 aagacttcgc ggccccgccg cgtcaagtgc tccgccagag agcgccccag aaagccgctc
     2521 ccgcccgcca gaatgattcg tcctcccgcc atccgagtct gctccttcat cgcgtcttac
     2581 gcccgacgag gaggcttcac tcaacctcca tgccgtcagg aacagtgtcg atttccgccg
     2641 gaagtttcgc gggacgatgg agagtcgcgt ggcaaaaggc gagtgacgcg gtcgcgtctt
     2701 ggcaaactgc gagaagtgta ggcagcaggc agcaggcagc aggcagtcaa gacagaagtc
     2761 aggagtcaga agatgcttga gaggtaaagt gaagccgtct tgctgttact gcttgaagag
     2821 cgaggaggtt cgggacgttg cctcccaagc ttcttcgtcc ttctgttttg actgcctgct
     2881 gcccgctgcc cgctgctcgc tctctgctgc ctactgcatt tcgaccgggt agcccgctgc
     2941 gacccaggcg gaggtgccgc ccgagacgtt gaagaggcgg aagccgcggg gcgcgagcag
     3001 gctggtggcg gcgctggaac ggtagccccc ggcgcagatg acggcgacgg ggcgcgcggg
     3061 gtcgaggtgc gcggcctctt cggcgaggcg cgggccgagg ggggcgctcg cggcgcgcgg
     3121 cgcgtggccc gaggtgtact cgccgggccg ccgcacgtcg agcacctgaa ggccgggttg
     3181 gtcgtccaac atctcgcgca gctcggcgac ggagatttgc gggatggtcg tcgcctcaag
     3241 ccctgcttcg cgccacgcgt cgatgccgcc gtcgaggtag ccgagcacgt tctcgtggcc
     3301 gacgcgcgcg agtcgcgtga cggcctcgtc aacctgctct tcggagtcgg cgacgatgac
     3361 gagcggcgtg ccgagcggga tgaggctccc ggcccacgag gcgaactgcc cgccgaggcc
     3421 gatgttgaga gcgccgctga cgtggcccgc gccgaagtcg gaggccgcgc gcacgtcgag
     3481 gacgacgtgg ccgccgccct gcgcgagcag aagcgcgacc tctcggggcg cgagcgcggc
     3541 ggggcgcggc agctcggcga gcgcggcggc ccccgcgcgg ttgatctccg cgtcgtgctg
     3601 gaagtaggcg gggcgctcgg gcaggtccgc ggtcatcagg cggacgaact cctctttggg
     3661 catcggctgg agcgcgtagt tgaagcggcg ctgctcgccc acggtcgagg aagtctcctt
     3721 cgagatgttg cgcccgcaca tcgagcctgc gccgtgcgcg gggtagacct ccacctcgtc
     3781 cgcgagcttc agtagcttac cgtgcagact gtcgtagagc atctcggcca tcatctcggt
     3841 cgtgtagccg cgcgcgcccg cgaggtcggg ccgccccacg tcgccgatga agagcgtgtc
     3901 gccggtcaag accttctgcg gctcgcggga gaccgccgtg tcgatgacga cgagcgagat
     3961 gccttcgggc gtgtggccgg gcgtctcgat gacgcgcagc aagacatcgc cgactttcag
     4021 ctcgtcgccc tccttcacgg ccctgtgcgg gacggtcgcg cccgcgcgcg cgccgaagac
     4081 gacctcggcg ccggtgcgct cggccagctc cctgtgcccg ctcacgaagt ccgcgtgcag
     4141 gtgcgtctcg acgacgtact tgatcttcag cccacggcct tcggcctccg caacgtactc
     4201 gtccacgtcg cgctgcggat cgacgaccgc ggcctcgccc ccggagccga tcaggtacga
     4261 ggcgtgcgcg agacagccga ggta
//
LOCUS       DBNGOH010000035         2902 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1105244, whole genome shotgun sequence.
ACCESSION   DBNGOH010000035 DBNGOH010000000
VERSION     DBNGOH010000035.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2902)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2902)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2902
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1105244"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..508)
                     /locus_tag="AC159P_00725"
     CDS             complement(<1..508)
                     /locus_tag="AC159P_00725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694001.1"
                     /translation="MNKRISSALASLFALALLLPAFVVAQQQGNTQPAPPAARRGETD
                     RTTPGSPSGNAGRSRRAARNGEALEGIEADVSEALTVIQENYVDGSKLDYNGVFKSSI
                     IGMLRSLDPHSNFYDAKEFEEQRADWRSEYYGIGATIGDRKVGGSTDTYVLATFEATP
                     AFRAGLRFG"
     gene            846..1280
                     /locus_tag="AC159P_00730"
     CDS             846..1280
                     /locus_tag="AC159P_00730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694002.1"
                     /translation="MSSNPYDPRNPGAPPEPIDDLMIPEDDSTEAGSDRERAVSSVRE
                     TASRIPEALSSIGRDISRTIERALSAKDDYVVAVKVSHEAQDSLEQLVQAGVFRNRAD
                     AAAFLIDEGIKAQHELFDRVRQKLSEIERLRAELRGMISKEQ"
     gene            1395..2843
                     /locus_tag="AC159P_00735"
     CDS             1395..2843
                     /locus_tag="AC159P_00735"
                     /inference="COORDINATES: protein motif:HMM:NF017044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HIR3694003.1"
                     /translation="MSSTPTLPAVAVEQFALVNGLRVVLSEDHSVPVVSVAVYYDVGS
                     RNEREGRTGFAHLFEHMMFQGSENVPKAGHFQYVFKNGGTMNGTTSSERTNYFETLPA
                     SRLPLALWLESDRMRSLKVTQENLDNQRDAVKEEKRLNYDNRPYSNAFLRLNELIYKN
                     PANAHSTIGSMEDLDEATIDDVREFFRVYYAPNNAVLTIVGDFDPAQARTLVEKYFAT
                     IPAQAPPPAVDVEEPGGVAAHHELYRDRFAQLPALMLGWQAPARRTPEFYALSHASDL
                     LLDGDSSRLYQRLVKDEESVVGIQGGIGERRGPSSLYVFAIPKPGHTTEEIRATIRSE
                     IERLAEEGPSAEEMMKLRNTLLNDSIRGRQSTLYRAQRLAEYALYDGDPALLNTEMER
                     YLAVTPEEIKRAVSRHLLNEDHSVMEVVPAQQAVEEAPSPAAPEPPGAPDQPGTPPPQ
                     VPPKPPAEPPPPTEPPLPTAEDGGAQMPSQAK"
BASE COUNT          540 a          997 c          921 g          444 t
ORIGIN      
        1 cgccgaagcg gaggccggcg cggaaggccg gcgtcgcctc gaaggtggcg aggacgtagg
       61 tgtcggtgct cccgccgacc ttgcggtcgc cgatggtggc gccgatgccg tagtactcgg
      121 agcgccagtc ggcgcgctgc tcctcgaact ctttcgcgtc gtagaaattc gagtgcgggt
      181 cgagcgagcg cagcatgccg atgatcgacg acttgaagac gccgttgtag tcgagcttcg
      241 agccgtcaac gtaattctcc tggatgacgg tcagcgcctc gctgacgtcg gcctcgatgc
      301 cttcgagcgc ctcgccattg cgcgcggcgc ggcgcgagcg gcccgcgttg cccgagggcg
      361 agccgggagt cgtgcggtcg gtctcgccgc ggcgggcggc gggcggcgcg ggctgcgtat
      421 tgccctgctg ctgtgcgacg acgaacgccg gaagaagaag cgcgagtgcg aacaggctgg
      481 cgagcgcgga agaaattctc ttattcacgg ggcaaacctc agttgtgacc acgttgaccc
      541 tttacagccc cgacccgttg ggagcgacga gggcgctgct accgaaaagc cgccgaagac
      601 cggcggggtt ggtgtagttt aattgatgag cacaccggcg gcaaggttgc gcgcccgcgc
      661 gacgaaaagt tttacaaaac cgtgatgaga aagagttaag gcgggcgctt gcgctcgttt
      721 cggcccaccc gtagaatgcc ttcagaaagc tttcacgcgc acagcaacgg gcacgagcag
      781 caaagtcgga cgcgaggcgc cgcgccgccg acgcgtcttc accccccgcg aggaggtttc
      841 gagcgatgag cagcaaccct tacgacccgc gcaaccccgg cgccccgccg gagccgattg
      901 acgatttgat gatccccgaa gacgactcga cggaggcggg cagtgaccgc gagcgcgccg
      961 tctcctcggt gcgcgagacg gcctcgcgca tccccgaagc gctctcctcc atcgggcgcg
     1021 acatcagccg caccatcgag cgcgcgctgt cggccaagga cgactacgtc gtggccgtca
     1081 aggtctcgca cgaggcgcag gacagcctcg aacagctcgt ccaggccggc gtcttccgca
     1141 accgcgccga cgccgccgcc ttccttatcg acgaaggcat caaggcgcag cacgagttgt
     1201 tcgaccgcgt ccggcagaag ctctccgaga tcgagcgcct gcgcgcggag ctgcgcggga
     1261 tgatcagcaa agaacaatga tgaacgatga acgcggaatg atgaataaga agcacgcggc
     1321 ttgtcatcct tcggcttgtc tttcattcat cattcatcat tccgcgttca tcattttctt
     1381 ttaagggggt tcatttgtcg tcaacgccca cactgcccgc cgtcgcggtc gaacagttcg
     1441 cgctggtgaa cggcctgcgc gtcgtcctca gcgaagatca ttcggtgccg gtcgtgtcgg
     1501 tcgcggtcta ctacgacgtg gggtcgcgca acgagcgcga ggggcgcacg ggcttcgcgc
     1561 acctgttcga gcacatgatg ttccaggggt cggagaacgt gccgaaggcc ggccacttcc
     1621 agtacgtctt caaaaacggc ggcacgatga acggcaccac gtcgagcgag cgcaccaact
     1681 acttcgagac gctgccggcc agccggctcc cgctcgcgct gtggctcgaa tcggatcgca
     1741 tgcgctcgct gaaggtgacg caggagaacc tcgacaacca gcgcgacgcg gtgaaggagg
     1801 agaagcggct caactacgac aaccgcccct actcaaacgc cttcctgcgc ctcaacgagc
     1861 tgatttataa gaaccccgcg aacgcgcact cgaccatcgg ctcgatggaa gacctcgacg
     1921 aggcgaccat cgacgacgtg cgcgagttct tccgcgtcta ttacgcgccg aacaacgccg
     1981 tgctcaccat cgtcggcgac ttcgacccgg cgcaggcgcg cactctcgtc gaaaagtatt
     2041 tcgccaccat cccggcccaa gccccgccgc ccgccgtgga cgtggaagag ccgggcggcg
     2101 tcgccgcgca ccacgagctg taccgcgacc ggttcgcgca actgcccgcg ctgatgctcg
     2161 gctggcaggc gcccgcgcgc cggacgcccg agttctacgc gctctcgcac gccagcgacc
     2221 tgctgctcga cggcgacagc tcgcgcctct accagcggct cgtcaaggac gaagagtccg
     2281 tggtcggcat ccagggcggc atcggtgagc ggcgcggccc ctcttcgctc tatgtcttcg
     2341 ccatcccgaa gccgggccac acgaccgaag agattcgcgc gacgattcgg tcggagatcg
     2401 aacgcctcgc cgaagagggg ccgagcgcgg aggagatgat gaagctccgc aacacgctcc
     2461 tcaacgactc gatccgcggt cgccagtcca cgctctaccg cgcgcaaagg ctcgccgagt
     2521 acgcgctcta cgacggcgac cccgcgctcc tcaacaccga gatggaacgc tacctcgccg
     2581 tcacgcccga agagatcaag cgcgccgtga gccgccacct gctcaacgaa gaccactccg
     2641 tgatggaggt cgtccccgcg cagcaggccg tcgaagaagc gcccagcccc gccgcgcccg
     2701 aaccgcccgg cgcgcccgac cagcccggca cgcctccccc gcaggtgccg ccgaaacctc
     2761 ccgcggaacc cccgccgccg acggagcctc ccctgccgac cgccgaggac ggcggcgcgc
     2821 agatgccttc gcaggccaag tgatgaacga tgaatgacag acagttttca gcttccggct
     2881 ttcagttttc agccggggca tc
//
LOCUS       DBNGOH010000036         3949 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_922126, whole genome shotgun sequence.
ACCESSION   DBNGOH010000036 DBNGOH010000000
VERSION     DBNGOH010000036.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3949)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3949)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3949
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_922126"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            217..2241
                     /locus_tag="AC159P_00740"
     CDS             217..2241
                     /locus_tag="AC159P_00740"
                     /inference="COORDINATES: protein motif:HMM:NF012698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HIR3694004.1"
                     /translation="MSSDIGHIYRFGDFAVDVDQRVLLRAGKPVPLTPKLFDTLLILV
                     ENGGRIVRKEELMGRLWPDTFVEEANLSVNIQQLRKALGDRARRPLYIETVARRGYRF
                     IADVEKVLNDSTVADAQSTRRLNTYDLLSGAGGNGEGRGEASWVGGEVGPGASDDLVK
                     TEYAQVDRTPARRPVRYTTAFVAAVVALALAACGWVLWDRYGAGPQPPDERVMLAVLP
                     FQNFTGDPDQDYFGDGLTEEMITQLGSLDPQRLGVIARTSAMHYKGSRLSLYQIGREL
                     GVQYLLEGSVRRDGDNVRITAQLIRVRDQTHIWARQYDRRLRDILVLQREIGQETANQ
                     IQLALGGDRPLTPGRQPRHAYQSYEAYDLWLKGQYFWNKRTAEGFRKAVDYFERAKEI
                     DPTYAHAYVGLANCYALMGGYTMRPQTEFMPKARAAALRALELDDGLAEAHTAMALIV
                     QNYELDWQTAEKEFRRAIELNPNYATAHHWYAEHLMWRGRFDEALRASEQARRLDPLS
                     LIISADHGAILYYARQYDSAIQQFRAVRELDPHNQRSGLIIHAYVEQGLFAEALADIE
                     AHQPPLGMNPPWYWSELAYAYGRSGQQAQARRALENLLELNRRQEVGAEAIIWAYIGV
                     GDKEQAMAWLEKAYAQRSPALTYLKVDPRYDALRGDPRFQELLRRVGFPP"
     gene            2518..2736
                     /locus_tag="AC159P_00745"
     CDS             2518..2736
                     /locus_tag="AC159P_00745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694005.1"
                     /translation="MRERVGLEGWIIDRALGVWTAPDEETARAEIAELCERRLARLDA
                     GRRLEATEAGASVAGGKAGLSLKFKSGE"
     gene            complement(2842..3183)
                     /locus_tag="AC159P_00750"
     CDS             complement(2842..3183)
                     /locus_tag="AC159P_00750"
                     /inference="COORDINATES: protein motif:HMM:NF021158.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HIR3694006.1"
                     /translation="MRTITGKIILPPNVPAAKADEVTIEVRDVSEADALSTVVAERRL
                     EDVALRPNGEIKFKLSVPDVGPNRSLAMRVHVSTDGSGRVKSGDLLTTTHIPVPSTGD
                     AEQLEVPVVVI"
     gene            complement(3180..>3949)
                     /locus_tag="AC159P_00755"
     CDS             complement(3180..>3949)
                     /locus_tag="AC159P_00755"
                     /inference="COORDINATES: protein motif:HMM:NF020032.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MvdC/MvdD family ATP grasp protein"
                     /protein_id="HIR3694007.1"
                     /translation="VAWWRRVRPYNVDRALVSPSARAFAESETAQAVGGMLDALACVW
                     VNPRGPDEAAHRKPYQWAVAREVGLRMPRTLVTNKPEAARAFVEEAGLGRTVFKAFLA
                     SLECWRETRLVGSADLEKIDLVRYAPVIFQEYVEGVDLRITVVGEEVFAAEIDARNTS
                     YPTDMRMVIGEAHLRAVELPPKVRKAILKLQRRLGLYYGAIDVRRTPDGEYIFLEVNP
                     AGQWLFVEQRTGLPITRAVADFLARLEDRPGTAGGAR"
BASE COUNT          721 a         1290 c         1267 g          671 t
ORIGIN      
        1 gtttcgaagg acggacgggg attgcccggt cagtcggggc cgcgttaata ttccgcgcgt
       61 ccgccacccc aaccgtgggt gatgccttct tccgctcctc cctgcccgac agccgggagt
      121 ttcaatcgcc agcacgtagc gcggagagta cgcgcccctt cgtgccatgt cataaaaagc
      181 ggctaagaga gttctaaaaa agttctaaat ccacccatgt cttccgacat cggccacatc
      241 tacaggttcg gagatttcgc ggtcgatgtg gaccagcgtg tcctcctgcg cgcgggcaag
      301 cctgttccgc tgacgccgaa gttgttcgac acattactca ttctggttga gaacggcggt
      361 cgaatcgtca ggaaggaaga gctgatgggg cggctctggc cggacacttt cgtcgaagag
      421 gcgaacttgt cggtgaacat ccagcagctc cggaaggcgc tcggcgaccg cgcccgccgg
      481 ccgctctaca tcgagactgt ggcgcggcgc ggctaccgct tcatcgcgga cgtggaaaaa
      541 gtcctgaacg actccaccgt cgcggacgcc cagagcaccc ggagattgaa tacttacgac
      601 ctcctttcgg gggccggggg caacggagag gggcgcggcg aagcctcctg ggtcgggggt
      661 gaagtcgggc caggggcttc ggacgattta gtgaaaaccg aatacgctca ggtagaccga
      721 acgccggcac gccggcccgt gaggtataca acagcatttg tcgccgctgt cgtcgctctc
      781 gccctggccg cgtgcggttg ggttctgtgg gatcggtacg gggccggacc tcagccgccg
      841 gacgaaaggg tgatgctcgc ggtcctcccc tttcagaact tcaccggcga ccccgaccag
      901 gactacttcg gcgacggtct caccgaagag atgatcacgc agctcgggag ccttgaccct
      961 cagcgcctcg gcgtcatcgc gcggacttcc gccatgcatt acaagggaag ccgtctttcc
     1021 ctgtaccaga tcggccgcga actgggcgtt cagtacctgc tcgaaggcag cgtgcggcgt
     1081 gacggggaca acgtccgcat cacggcccag ctcatacggg tccgcgatca gacacacatt
     1141 tgggcgcgcc agtacgatcg ccggctgcga gacatccttg ttcttcagcg cgagataggg
     1201 caggagaccg ccaatcaaat tcagctcgcg ctggggggag acagaccgtt aaccccgggc
     1261 cgccagcccc gtcacgccta ccagtcttac gaggcctacg acctctggct caaaggccag
     1321 tacttctgga acaagcggac cgccgaaggt ttccgcaagg cagtcgacta cttcgagcgg
     1381 gccaaagaga tagacccgac ttacgcgcac gcctacgtcg gcctggcgaa ttgctacgcc
     1441 ttaatgggcg gctacacgat gcggccgcag accgagttca tgccgaaggc gcgggcggcg
     1501 gcgttgcggg cgctggagct tgacgatggc ctggcggaag cccacaccgc catggctttg
     1561 atcgtccaga actacgagct ggactggcag accgcggaga aggagttccg ccgcgccatc
     1621 gaactgaacc ccaactacgc cacggcccac cactggtatg ccgagcacct gatgtggcgg
     1681 ggccggttcg acgaggcgct ccgcgcgagc gagcaggcgc gccggctcga ccctctctcg
     1741 ctgatcatct ccgccgacca cggggcgatc ctgtattacg cgcgtcagta cgacagcgcc
     1801 atccaacaat tccgcgccgt gcgcgagctg gaccctcaca accagcgcag cggcctcatc
     1861 attcatgcct acgtcgagca agggttattc gcggaagcgc tcgccgacat cgaggcgcat
     1921 cagcctccct tgggtatgaa tccaccctgg tattggtcgg agttggccta cgcctacggc
     1981 cgctcgggcc agcaggcgca ggcccggcgc gcgctggaga atctgctgga gttgaaccgg
     2041 cggcaggagg tcggagccga agcgattatc tgggcgtaca tcggcgtcgg cgataaggaa
     2101 caggccatgg cctggctgga gaaggcatac gcgcagcgct cacccgctct cacttacctg
     2161 aaggtggacc cccgctacga cgcgctgcgc ggcgacccgc gctttcagga gttgctgcgg
     2221 cgcgtcggtt ttccaccctg aactctaaag tacggcgcac ttaacaatgc cgtgcagccg
     2281 accgcgaatc agcgtgcctc ttaccgagaa cttgtccgta tcggcgttat gtgcgcggcg
     2341 gcaacacgaa ggtcaagcgt caagcgtcaa gcagttgcac gctggaggct gacgtccagc
     2401 agtgcaaatg atggcgtgta atctgcacgg caatcgaact cgagtgcgtg gctatgaata
     2461 tccatgccgg gagccccacc agaaacgggt caacgttact aagcgacagg atttcacttg
     2521 cgcgagcgag tggggctgga gggctggatc atcgaccggg cgctgggcgt gtggactgct
     2581 ccggacgagg agacagcgag agcggagatc gccgagctgt gcgagaggcg gctggcgcgg
     2641 ctcgacgccg ggcgccggct ggaggcgacc gaggcgggtg cgtctgtagc tggaggtaaa
     2701 gccggcctga gcctcaagtt caagtccggc gaatagaccg gacgccggcc aagcacgccg
     2761 gggagacctc cctagccgcc gcgctcccgt ccgtcctttg agctgctttg gaaccgccgg
     2821 caatccgatg tgagcaccgt ctcagataac gacgaccggg acttcgagtt gctccgcatc
     2881 ccccgtgctc gggacaggga tgtgtgtagt agtgagcagg tctcccgact tgacgcgccc
     2941 gctcccgtcc gtgctgacgt gcacccgcat cgcgagtgac cggttggggc caacgtctgg
     3001 gaccgacaac ttgaatttga tctccccgtt cggcctcagg gcaacatcct cgagccgacg
     3061 ctctgcgacg actgtcgaga gagcgtcggc ctcggagacg tcgcggacct cgatcgtaac
     3121 ttcatccgcc ttcgcggccg ggacgttcgg tggcaagatg atcttgcctg tgattgtcct
     3181 catcgtgcgc ctcccgctgt tccgggccga tcctcaagcc tggccaggaa gtcggcaacg
     3241 gcccgggtga taggcagccc cgtccgctgc tccacgaaca gccactgccc tgccgggttc
     3301 acctcgagga aaatatactc cccgtcgggc gtgcggcgca cgtcgatggc cccgtagtac
     3361 agtccaagcc ggcgctggag cttcaggatc gccttgcgca ctttgggggg cagctcgacg
     3421 gcccgcaggt gggcctcgcc tatgaccatc cgcatgtcag tggggtagga tgtgttgcgc
     3481 gcgtcgatct cggcggcgaa cacctcctcg ccgacgaccg taatgcgcag gtcaaccccc
     3541 tcgacgtact cctggaagat gaccggtgcg taacgcacca aatcgatctt ctccaagtcc
     3601 gcgctcccga ccaggcgcgt ctcgcgccag cactcaaggg aggcgaggaa ggccttgaac
     3661 accgtccgcc ccagaccggc ctcttcgacg aaggcgcgcg cggcttccgg cttgttggtc
     3721 acgagcgtgc gcggcatccg caggcccacc tcgcgggcga ccgcccactg gtacggcttg
     3781 cggtgggccg cctcatccgg cccgcgcggg ttcacccaga cgcacgcgag cgcgtcgagc
     3841 atcccgccga cggcctgtgc cgtctcgctc tcggcgaagg cgcgcgcgga cgggctgacc
     3901 agcgcccggt cgacgttgta cgggcgcacc ctgcgccacc acgcgacgc
//
LOCUS       DBNGOH010000037         3356 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4771404, whole genome shotgun sequence.
ACCESSION   DBNGOH010000037 DBNGOH010000000
VERSION     DBNGOH010000037.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3356)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3356)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3356
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4771404"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..613
                     /gene="pyrH"
                     /locus_tag="AC159P_00760"
     CDS             <1..613
                     /gene="pyrH"
                     /locus_tag="AC159P_00760"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020676544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HIR3694008.1"
                     /translation="ELGVEVAVVVGGGNIFRGVSESAGNMDRGAADYIGMLATVMNSV
                     VLQDALEKTGVYTRVLSAINIPQVAEPFIRRRAIRHLEKGRVVIFAAGTGNPYFTTDT
                     AAALRALEINAEVILKATKVEGVYSADPVKDPSATRYDCITYQEVLERQLKVMDASAI
                     SLCMDNDLPIIIFNMRRPGNIMRVVTGEEGVGTRVCLNKPEGK"
     gene            689..1249
                     /gene="frr"
                     /locus_tag="AC159P_00765"
     CDS             689..1249
                     /gene="frr"
                     /locus_tag="AC159P_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013377096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HIR3694009.1"
                     /translation="MSEKDVIKETRPRMEGAVEDLRKKLATVRTGRAAVSLLDPVTVD
                     YYGTPTPLSQMASVHAPEPTLLTVQPWDQSQLGAIEKAIRSSDLGLNPTNDGKMVRVP
                     VPPLTEERRKQLAKQVHEYAEEHRTAIRNVRRDANDRLKRMLKDKAISEDAERDALEE
                     VQKLTNTYVGRVDELARTKEQEILSV"
     gene            complement(1471..>3356)
                     /locus_tag="AC159P_00770"
     CDS             complement(1471..>3356)
                     /locus_tag="AC159P_00770"
                     /inference="COORDINATES: protein motif:HMM:NF051698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HIR3694010.1"
                     /translation="LPTFQVSEAFTGGGAPLGFTSYSEKRWELQNYTTHTRGAHTFRF
                     GARLRGVRLTDITDTNFNGTVLVSGGVAPRLDAAGNVIIGGDGLPVLDPITSLERLQR
                     TLDLQRDGFTGAALVARGGGAAQFTLAGGETEARVSQFDFGGFFQDEWRIRPNLLLTL
                     GLRYENQSNLSSHVNLGPRIFFAWAPGGTTTGSLLGGPNQPKFVIRGGFGVFYDRFGE
                     NGTLQALRFGGDGQDRFVVDDPDILGNIVLTPTGVVPGSIPTVGELSAFLQPQAITRI
                     ADNLEAPRTVLTALQVERQLPKNFTVFALFFNYRQQRVFRQRNVNACLPGTFVRGVSC
                     DRPDDAAGDIYQYESTGKFNDYRFQMGIRNQLRPGFSLFANYNTGKATADTDCIFGGL
                     GGCFPADSYDLSGENGRVAFFSRHQVFIGGNIAIPKLKLSLNPFVTARTGQFFNITTG
                     IDRNGDGIFNDRPAFADAQTPAADLRVTEWGNFDIRPKSGQTIIPRNYGEGPSTIAIN
                     LGISRTFSFGDMPRPAGAAPAGAAPAGGPQGGGGAPARQAGGPGPGGPQGAGGGGEKR
                     YSLTFSMNIQNVLNRVNLATPVGNLSSPLFGESTRIVGPFGGFGATAGGNRKIQFSTR
                     FNF"
BASE COUNT          582 a         1016 c         1226 g          532 t
ORIGIN      
        1 cgagctgggg gtcgaggtcg cggtcgtcgt cggcggcggc aacatcttcc gcggcgtgtc
       61 ggagagcgcg ggcaacatgg accgcggcgc cgccgactac atcgggatgc tggcgaccgt
      121 gatgaactcg gtcgtgttgc aggacgcgct cgaaaagacg ggcgtctaca cgcgcgtcct
      181 ctcggccatc aacatcccgc aggtggccga gcctttcatc cgccgccgcg cgatccggca
      241 tctcgaaaag ggccgcgtcg tcatcttcgc cgccggcacc ggcaacccgt atttcacgac
      301 cgacacggcg gcggcgctgc gcgcgctcga aatcaacgcc gaggtgatcc tgaaggcgac
      361 gaaggtcgag ggcgtctact ccgccgaccc ggtcaaagac ccgtccgcca cgcgctacga
      421 ctgcatcacg taccaggaag tgctcgaacg tcagctcaag gtgatggacg cctcggccat
      481 cagcctgtgc atggacaacg acctgccgat catcatcttc aacatgcgcc ggcccggcaa
      541 catcatgcgc gtcgtcaccg gcgaggaggg cgtcggcacg cgcgtctgtc tcaacaaacc
      601 ggagggtaaa tagtaaatgg tgaatggtga atagaaagaa tctttcacca tttactattt
      661 accattcacc atttactcgg agagagccat gagcgaaaag gatgtcatca aggagacgcg
      721 cccgcggatg gagggggcgg tcgaggactt gcgcaaaaaa ctggcgaccg tgaggacggg
      781 gcgcgcggcg gtgagtttgc tcgaccccgt gacggtcgat tactacggga cgccgacgcc
      841 cttgagccag atggcgtcgg tgcacgcgcc ggagccgacg ttgctgaccg tgcagccgtg
      901 ggatcagtcg cagctcggcg ccatcgagaa ggcgatccgc tcgtccgacc tcggactcaa
      961 cccgacgaac gacgggaaga tggtgcgcgt ccccgtcccg cccttgaccg aggagcggcg
     1021 caagcagctc gccaagcagg tgcacgagta cgcggaggag caccgcaccg cgatccgcaa
     1081 cgtccgccgc gacgccaacg accggctgaa gaggatgctc aaggacaagg ccatctccga
     1141 ggacgccgag cgcgacgcgc tcgaagaggt gcagaagctg accaacacct acgtcggcag
     1201 ggtggacgag ctggcgagga cgaaggagca ggagattcta agcgtgtagg cagtcggcag
     1261 tcggcagtca agacagaaga cagaagaggc ttgagagcta acgtctcaag cctcttgcta
     1321 ttttgcttga agaacgaaga ggctcgggac tttaccaccc aagcctcttg ctttattacg
     1381 tgacgagcgg agaggcttgg gacgttacct ctcaagcctc tccgcccttc tgtctttgac
     1441 tgcctacagc ctactgcccg ctgcctactg ttagaagttg aagcgggtgc tgaactgaat
     1501 cttgcggttg ccgccggcgg tggcgccgaa gccgccgaag gggccgacga ttctggtgga
     1561 ctcgccgaag agcggcgagc tgaggttgcc gacgggcgtc gccagattca cgcggttgag
     1621 cacgttctgg atgttcatcg agaaggtcag gctgtagcgc ttctcgccgc cgccgcccgc
     1681 gccctgcggg ccgcccggcc cggggccgcc ggcctgcctc gcgggggcgc cgccgccgcc
     1741 ctgcgggccg cccgccggag cagcgcccgc gggcgcggcg ccagccggtc gcggcatgtc
     1801 gccgaagctg aaggtgcggc tgatgccgag gttgatggcg atggtgctcg ggccttcgcc
     1861 gtagttgcgc gggatgatgg tctggccgga cttgggcctg atgtcgaagt tgccccactc
     1921 ggtgacgcgg aggtcggcgg cgggcgtctg ggcgtcggcg aaggcggggc ggtcgttgaa
     1981 gatgccgtcg ccgttgcggt cgatgccggt cgtgatgttg aagaactggc ccgtgcgcgc
     2041 ggtgacgaac gggttgagcg agagcttgag tttggggatg gcgatgttgc cgccgatgaa
     2101 gacctgatgg cgcgagaaga aggcgacgcg cccgttctcg ccgctgaggt cgtaggagtc
     2161 ggcggggaag cagccgccga ggccgccgaa gatgcagtcg gtgtcggcgg tcgccttgcc
     2221 cgtgttgtag ttggcgaaga gcgagaagcc ggggcgcagt tggttgcgga tgcccatctg
     2281 gaagcggtag tcgttgaact tgcccgtgga ctcgtactgg tagatgtcgc ccgccgcgtc
     2341 gtcgggccgg tcgcagctca cgccgcggac gaaggtgccg ggcaggcagg cgttgacgtt
     2401 gcgctggcgg aagacgcgct gctggcggta gttgaagaag agggcgaaga ccgtgaagtt
     2461 cttcgggagc tgccgctcga cctgcaaggc ggtgaggacg gtgcgcggcg cttcgaggtt
     2521 gtcggcgatg cgcgtgatgg cctgcggctg gaggaacgcg gacaactcgc cgacggtcgg
     2581 gatgctgccg gggacgacgc cggtcggcgt caggacgatg ttgcccagaa tgtccgggtc
     2641 gtcaacgacg aagcggtctt gcccgtcgcc gccgaagcgg agcgcctgga gcgtgccgtt
     2701 ctcgccgaag cggtcgtaga agacgccaaa gccgccgcgg atgacgaact tgggctggtt
     2761 ggggccgccg agcagcgagc cggtggtggt gccgcccggc gcccacgcga agaagatgcg
     2821 cggcccgagg ttgacgtggc tgctcaggtt cgactggttc tcgtagcgca ggccgagcgt
     2881 gagcagcagg ttcgggcgga tgcgccactc gtcctgaaag aagccgccga agtcgaactg
     2941 gctgacacgc gcctcggtct cgccgccggc gagggtgaac tgcgccgcgc cgccgccgcg
     3001 cgcgacgagc gccgcgcccg tgaagccgtc ccgctgcaaa tcgagcgtgc gctggaggcg
     3061 ctcaaggctg gtgatggggt cgagcacggg caggccgtcg ccgccgatga tgacgttgcc
     3121 cgcggcgtcg agccgcgggg cgacgccgcc cgagacgagc accgtgccgt tgaagttggt
     3181 gtcggtgatg tcggtcaggc gcacgccgcg cagccgcgcg ccgaagcgga aagtgtgcgc
     3241 gccgcgcgtg tgcgtcgtgt agttctggag ttcccagcgc ttctcgctgt aggacgtgaa
     3301 gccgagcggc gcgccgccgc ccgtgaacgc ctcgctgacc tggaaggtgg gcagca
//
LOCUS       DBNGOH010000038         1575 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2022184, whole genome shotgun sequence.
ACCESSION   DBNGOH010000038 DBNGOH010000000
VERSION     DBNGOH010000038.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1575)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1575)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1575
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2022184"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            99..512
                     /locus_tag="AC159P_00775"
     CDS             99..512
                     /locus_tag="AC159P_00775"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013052.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein"
                     /protein_id="HIR3694011.1"
                     /translation="MPDKLLEVRKSSIYGKGCFALKHFPARKKIALYEGELVKGNRRI
                     EERLWEQEAVNKVIRMGKNLAIDGSIGGNATAFINHSCEPNAYMRTVPGDKVAFFALR
                     DIQPGEEITMDYRDPNHPLVCKCGAPRCRSAKQRA"
     gene            843..>1575
                     /locus_tag="AC159P_00780"
     CDS             843..>1575
                     /locus_tag="AC159P_00780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694012.1"
                     /translation="MKRRLFRAVPLCLAFALLVVGGACLPPPAADAVRSDGLVVHEWG
                     TFTSFAGRNGEALSWRPLSFESDLPGFVYSIDKGATWESSGLRYPSKSSRFVTVRMET
                     PLLYFYAGRETEVSVKVGFNGGTITEWYPQARAGGKTVEWAQFRVLPGARDVELAHEG
                     GPNHYYPARDTDAATVRVRGERGAEHEKFLFYRGVGDSRLPLAVLVEGGKVVVRSTEA
                     RGVGKVILFERRGGAAGHSVGDAPAG"
BASE COUNT          267 a          535 c          510 g          263 t
ORIGIN      
        1 gtcctcagtc ctccgctcct cagcacttta ccttcgcctc ggttataatc gcgcccgctc
       61 tgctcacatc gtttcgacat ctcggggaga ctacacgcgt gccggataag ttgttggagg
      121 tcaggaagag cagcatctac gggaagggtt gtttcgcgct caagcacttc cccgcccgca
      181 agaagatcgc gctctacgag ggcgagctgg tcaagggcaa ccgccgcatc gaggagcggc
      241 tgtgggagca ggaggccgtc aacaaggtca tcaggatggg caagaacctc gccatcgacg
      301 gctccatcgg aggcaacgcg accgccttca tcaaccactc gtgcgagccg aacgcctaca
      361 tgcgaaccgt gcccggcgac aaggtcgcct tcttcgccct ccgcgacatc caacccggcg
      421 aagagatcac catggactac agagacccca accacccgct cgtctgcaag tgcggcgccc
      481 cgcgctgccg ctccgccaag cagcgcgcgt gagccttcat caacagacgt tcaccacgaa
      541 gacacgaagg aagagcaacc ggaaggaatg aaccttcccg ctctcttttc ttcgtgcgtc
      601 ttcgtgtctt cgtggtgaag gtcgcccgcc gcccgcccca caaaaaaaag ggatgagtat
      661 tcgacgcccc cgcgtctaag tgacgccggg ataaaaaccc gcgcgcaaaa ttcgggcggg
      721 ccgtgcgttc aagggcagtg ccgcctgtaa cgggatggtc tgtccgcgcg cggctgccga
      781 ctctctcccc gaccgccttt cgtgtccgag acgtaccgaa tcattagtgg aggttgtgta
      841 tgttgaagcg tcgtctgttt cgcgccgtcc cgctgtgcct cgcgttcgct ctgctcgtcg
      901 tcggcggggc gtgtctgccg ccgcccgccg ccgacgccgt gcggagcgac gggttggtgg
      961 tgcacgagtg gggcactttc acttcgttcg cgggccgcaa cggcgaggcg ttgtcgtggc
     1021 gcccgctcag tttcgagagc gacctgcccg gcttcgtcta cagcatcgac aagggcgcca
     1081 cgtgggagag cagcggcctg cgctaccctt cgaagagcag ccgcttcgtc accgtccgga
     1141 tggagacgcc gctcctctac ttctacgccg ggcgcgagac ggaggtctcg gtcaaggtcg
     1201 gcttcaacgg cgggacgatc accgaatggt atccgcaggc gcgcgcgggc gggaagaccg
     1261 tcgagtgggc gcagttccgc gtgctgcccg gcgcgcgcga cgttgaactc gcgcacgagg
     1321 gcggcccgaa ccactactac cctgcgcgcg acacggacgc cgccaccgtg cgcgtccgcg
     1381 gcgagcgcgg ggccgagcac gagaagttcc tcttctaccg cggcgtcggc gactcgcgcc
     1441 tgccgctcgc agtcctcgtc gaaggcggca aggtggtcgt gcggagcacc gaggcgcgcg
     1501 gcgtcggcaa ggtcatcctc ttcgagcgga ggggcggcgc cgcgggccac tccgtcggcg
     1561 acgcgcccgc gggcg
//
LOCUS       DBNGOH010000039         3471 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1289219, whole genome shotgun sequence.
ACCESSION   DBNGOH010000039 DBNGOH010000000
VERSION     DBNGOH010000039.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3471)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3471)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3471
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1289219"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            452..1948
                     /locus_tag="AC159P_00785"
     CDS             452..1948
                     /locus_tag="AC159P_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012031835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HIR3694013.1"
                     /translation="MMKLLRVKPMAALALLWSVGGSLTTLAQTAELSIEERLAKAEAA
                     AASAQTAGDNAWMLVCAALVLMMTGPGLALFYGGLVRKKNVLSTMMQSFILMAVVTVI
                     WALYGYSIAFSEGTPFFGGLGYALLNGVGAEPNADYAATIPQQTFMLFQLMFAIITPA
                     LITGAFAERIKFSAMLLFSVLWVTVVYFPLAHMVWGKGGFLNAVLGGVVPALDFAGGT
                     VVHIASGFSALVCAIYLGKRIGYPKTMMAPHNLVLSIIGAALLWVGWFGFNAGSALAA
                     GSLASSAFTATHFAAAAATLGWVAAEWVRTGKPTVLGAISGAVCGLVGITPASGFVTP
                     MSSLIIGFIAGVGCFLMVTEVKKRLGYDDSLDVFGVHGTGGTLGALLTGVFATSAVNP
                     IFKDAAGNPLPVGLVDGNGAQVLNQLAGVGITVALAVVGSLVLLKIVDLVVGLRVSGE
                     EEMQGLDLSQHGEDGYNLDLDLVPALAGSESHAGHAPVGAASVMAEQA"
     gene            2415..>3471
                     /locus_tag="AC159P_00790"
     CDS             2415..>3471
                     /locus_tag="AC159P_00790"
                     /inference="COORDINATES: protein motif:HMM:NF028442.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HIR3694014.1"
                     /translation="MSLFLVAHLLAPAGVLAQGARRGAAAAGGRQSSAPLAWPRVTRQ
                     SKPWTRWWWLGNVAEKRELTEQLKEYERAGLGGVEITPIYGVKGHERRFVDFLSPAWV
                     DLLEHTLREAERLGMGVDMATGTGWPFGGPWVGADDAPRYFAHQSYSLKGGGRLEEPV
                     RYVQKPLVRAVGQRPDIRELKEPVSANANLQALALDQVRFEKPLPLQVLMAYSDKSET
                     LDLTGRVGADGRLDWVAPAGDWKLYALFQGWHGKMVERAAPGGEGNVIDHFSDEALKN
                     YLKKFDRAFAGRNIGSLRAFFNDSYEVDDAEGQADWTPRFFEEFRARRGYDLRRHLPA
                     LAGDASDEEDARVIYDLR"
BASE COUNT          563 a         1108 c         1223 g          577 t
ORIGIN      
        1 ggccgcgcgt gggtcgcgtc ggcgccgcgc gccgaatgca gggggacggc acacgcggcg
       61 cggcggtcgg tttgcattct atctcgcctc cgagccggaa acagcaggaa agcgaaaaat
      121 atttgacggc ctcttcggcg gcggcgacat ttttgtcgcg acggctgcgg tctccgacaa
      181 agatgtgtcg ggagcaggcg cgtccgcacg gcccgcctct gcaacccccg aaccgccgcg
      241 ccccttcttc tctcaacaaa ttgctaggca tagctcatgc caatggccgg cgcgggggcg
      301 cggggtctga gtggcacgat ggttgttaag ggcatcgtcg tcaagaagcg catcgggttc
      361 gcggcgccgc cggttcgcgc ggcggcgtga accgtcaaga aagattcctt cagcatatta
      421 agtctcaaga gaaagattga ggagagatca catgatgaaa ctcctgcggg tgaagccgat
      481 ggcggcgctg gcgctgctct ggtctgtcgg cggctcactg acgacgctgg cccagacggc
      541 ggaactatcc atcgaggagc ggctggcgaa ggccgaggcc gccgccgcga gcgcgcagac
      601 ggcgggcgac aacgcgtgga tgctggtctg cgcggcgctc gtgctgatga tgacggggcc
      661 gggcctcgcg ctcttctacg gcgggctggt gcggaagaag aacgtcctct cgacgatgat
      721 gcagagcttc atcctgatgg ccgtcgtgac ggtcatctgg gcgctctacg gctacagcat
      781 cgcgttcagc gagggcacgc cgttcttcgg cggactcggc tacgcgctcc tgaacggcgt
      841 cggggccgag ccgaacgcgg actacgcggc gaccatcccg cagcagacct tcatgctctt
      901 ccaactgatg ttcgccatca tcacgcccgc gctcatcacc ggcgccttcg ccgagcgcat
      961 caagttcagc gcgatgctgc tgttcagcgt gctgtgggtg acggtcgtct acttcccgct
     1021 ggcgcacatg gtctggggca agggcggctt tttgaacgcg gtgctgggcg gcgtcgtccc
     1081 ggcgctcgac ttcgcgggcg gcaccgtggt gcacatcgct tcgggcttct cggcgctggt
     1141 gtgcgccatc tacctcggca agcgcatcgg ctacccgaag acgatgatgg cgccgcacaa
     1201 tctggtcttg agcatcatcg gcgcggcgct gctgtgggtc ggctggttcg gcttcaacgc
     1261 gggcagcgcg ctcgccgccg ggagcctcgc ttcgagcgcg ttcacggcga cgcacttcgc
     1321 ggcggcggcg gccacgctcg gctgggtcgc ggcggagtgg gtgcgcaccg gcaagccgac
     1381 cgtgctcggc gcgatctcgg gcgccgtgtg cgggctggtc ggcatcacgc ccgcctcggg
     1441 cttcgtcacg ccgatgtcga gtttgattat cggcttcatc gcgggcgtcg gctgcttcct
     1501 gatggtgacg gaagtgaaga agcggctcgg ctacgacgac tcgctcgacg tgttcggcgt
     1561 ccacggcacg ggcggcacgc tcggcgcgct cctgaccggc gtgtttgcca ccagcgccgt
     1621 caaccccatc ttcaaggacg cggctggcaa cccgctgccc gtcggcctgg ttgacggcaa
     1681 cggcgcgcag gtcttgaacc agctcgcggg cgtcggcatc acggtcgcgc tcgcggtcgt
     1741 cggctcgctc gtcctgctga agatcgttga cctcgtcgtc ggtctgcgcg tcagcggcga
     1801 agaggagatg cagggtctcg acctcagcca gcacggcgag gacggctaca atctcgacct
     1861 cgacctcgtc ccggcgctcg ccgggagcga gagccacgca ggccacgcgc ccgtcggcgc
     1921 cgcgagcgtc atggccgagc aggcgtaacg actcaagccc cgccaccggt aaagctgcgg
     1981 caaacccctc ctcttaccgg acaccaaagc ggcctacctg tttttcgcgg gacaggtagg
     2041 ccgcttctct ttgtgcggcg gaaggcgaag aggagcgggg gaaagggcga aaagggaagc
     2101 aaggagttgc acaggcgcgg ggcctaaccc gtgaagtcag gttgtgaagc gactttccgc
     2161 cgcgagcgaa ttctgtggtg aaagacgggt ggcagcattt tagcggcagc atgatatatt
     2221 tctgccgacc acgcaaaata gcgagagcgg ttgcgccgga aagtgaaact cgaaacgttc
     2281 gccgccgcgg agtttcacct tgaaggcaga agtcgggtcg ctcttcgcca tgctccccgt
     2341 tctcggcaca ggcgatcata tccggaggtg ggttcgtgaa ggacaagaga cgattctcga
     2401 aggctcgcgt tctcgtgtcg ctgttcctgg tcgcacacct gctcgccccc gccggcgtcc
     2461 tcgcgcaggg cgcccgccgg ggggccgcgg cggcgggagg gcggcaaagc tcggcgccgc
     2521 tcgcgtggcc gcgggtcacg aggcagagca agccctggac gcgctggtgg tggctgggca
     2581 acgtcgccga aaagagggag ttgaccgagc agttgaagga gtacgagcgc gccggcctgg
     2641 gcggcgtcga gatcacgccc atctacggcg tcaagggcca cgagcgccgc ttcgtcgatt
     2701 tcctttcgcc cgcgtgggtc gatctgctgg agcacacgct gcgcgaggcc gaacggctcg
     2761 gcatgggcgt ggacatggcg acggggacgg ggtggccctt cggcgggccg tgggtcggcg
     2821 cggacgacgc ccccagatat ttcgcccacc agtcctactc gttgaagggc ggcgggcggc
     2881 tcgaagaacc cgtccgctac gtgcagaagc cgctcgtgcg cgcggtcggc cagcgcccag
     2941 acatccgcga gctgaaggag cctgtcagcg ccaacgcgaa tctgcaagcg ctggcgctcg
     3001 accaggtcag gttcgagaag ccgctgccgc ttcaggtttt gatggcctac tcggacaaga
     3061 gcgagacgct cgacctgacg ggccgggtcg gcgccgacgg caggctcgac tgggtcgccc
     3121 cggcgggcga ctggaagctc tacgcgctct ttcagggctg gcacgggaag atggtcgagc
     3181 gcgccgcgcc gggcggcgag gggaacgtca tcgaccattt ctccgacgag gcgctcaaaa
     3241 attacctcaa gaagttcgac cgggcgttcg ccgggcgcaa catcggaagc ttgcgcgcct
     3301 tcttcaacga ctcctacgag gtggacgacg cagaggggca ggccgactgg acgccccgct
     3361 tcttcgagga gttcagggcg cggcgcggtt acgacctgcg ccgccacctt ccggcgctcg
     3421 ccggcgacgc ctcggacgaa gaggacgcgc gcgtcatcta cgacctgcgc g
//
LOCUS       DBNGOH010000040         5232 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_556116, whole genome shotgun sequence.
ACCESSION   DBNGOH010000040 DBNGOH010000000
VERSION     DBNGOH010000040.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5232)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5232)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5232
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_556116"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..336)
                     /locus_tag="AC159P_00795"
     CDS             complement(<1..336)
                     /locus_tag="AC159P_00795"
                     /inference="COORDINATES: protein motif:HMM:NF024273.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HIR3694015.1"
                     /translation="MALLEPGEAASAYKAKILSYQAGADFLALQASAPAKLASLVDGL
                     SAEELARRPAPEKWSIQELVAHLADDELVGGYRIRMILSAPGTPIQAFDQDVWARTGR
                     YDKTDVRSSL"
     gene            complement(637..786)
                     /locus_tag="AC159P_00800"
     CDS             complement(637..786)
                     /locus_tag="AC159P_00800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694016.1"
                     /translation="MDEAPREVVQITADAGGGYLINVEVPAGSLAQLAKAYGEEADGE
                     PTLQS"
     gene            complement(825..1046)
                     /locus_tag="AC159P_00805"
     CDS             complement(825..1046)
                     /locus_tag="AC159P_00805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694017.1"
                     /translation="MDNLQTRLQAKAEGAARILSDIAEDESKPASVRVAAARIIIESA
                     LKIHEQAELGVRLKAIEDALEAQKRGGRR"
     gene            complement(2173..2364)
                     /locus_tag="AC159P_00810"
     CDS             complement(2173..2364)
                     /locus_tag="AC159P_00810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694018.1"
                     /translation="MKREVETIRRTRATLDAETRERLARLLREIFGHAPSGRVILHAH
                     AGGVLHAELEEKQPRGAKT"
     gene            complement(2361..3230)
                     /locus_tag="AC159P_00815"
     CDS             complement(2361..3230)
                     /locus_tag="AC159P_00815"
                     /inference="COORDINATES: protein motif:HMM:NF020811.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HIR3694019.1"
                     /translation="MNGCQWPRAAEGTPILAVWGVCDGICDCPKGSECPSPGKHPYPK
                     FCPNGVYSATTDAATFRRWHAIDPRINFGEAMGEVIRIDVDPRNGGDATYFDPEEAHG
                     EFTPTREKKTGGGGRHKKYRLPETIKRAKGELKCKLGPGVDVKAAGGYIIAPGSSHVS
                     GRPYGFETDSPVADAPEWIVETLKKAAAGESPPPPIDFQDYRDRRRTSTGGARFFGKR
                     ERNDGLRDVMLGRWTHGYAEDASDLYRQVLEVRDTRCEHDPADPPPSDRDIWDLVQRT
                     VRNYARGERGGAA"
     gene            3579..3971
                     /locus_tag="AC159P_00820"
     CDS             3579..3971
                     /locus_tag="AC159P_00820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694020.1"
                     /translation="MLLLALATLLLAAGVAAAQEETTLKDTTPKRRLRSPASARGFIG
                     GESHDSYVVRARRGQTLTVRISWRREGDNRAEFSVSESPDFYTAEPAEFGAASGGRWS
                     GKVPRSGDYYIYVVAHPTARYTLRVRVE"
     gene            4147..4539
                     /locus_tag="AC159P_00825"
     CDS             4147..4539
                     /locus_tag="AC159P_00825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694021.1"
                     /translation="MFVCTVAGVALLCFGWTAYRRAEEQSVLRTHSGLLALLVAGDYE
                     RAYRLTTDEYRAAHTVGEFQARFRHYEGEKHYLKRSPTVLSLGLRSAEVYADEVRSLI
                     EFLNGPSFFYRKERGQWRFTGEVAHYMD"
     gene            complement(4545..4904)
                     /locus_tag="AC159P_00830"
     CDS             complement(4545..4904)
                     /locus_tag="AC159P_00830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694022.1"
                     /translation="MNGQHTTDALPLGLLELDPTGIVIRYAPTPRASLRRERVVGRDF
                     FTEVMPAEQLYGFRARFLAFMDGGQQQERFALSVPRDGGALRVQVVLAAHGEQRVGRR
                     ERLALVSITPEQPRAAA"
     gene            complement(4971..5180)
                     /locus_tag="AC159P_00835"
     CDS             complement(4971..5180)
                     /locus_tag="AC159P_00835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694023.1"
                     /translation="MLKPLTTEEMVQVSLATRGECYAHRRWPEEVVRAFAAAEVITAR
                     ETQEGRAPHSPRLFRRMLSFAHSST"
BASE COUNT          845 a         1737 c         1666 g          984 t
ORIGIN      
        1 cagcgacgag cgtacgtccg tcttgtcgta ccttccagtg cgcgcccata catcctgatc
       61 gaacgcctga attggtgtcc caggtgccga caggatcatt cggattcgat agccgccgac
      121 gagttcatca tcggcaaggt gagcgacaag ctcctgaatc gaccacttct ccggcgccgg
      181 gcgtcgggca agctcttccg ctgagagccc atcgacgagg gacgcgagct ttgccggcgc
      241 ggacgcctgc agagcaagga aatccgcacc cgcctggtag cttaggatct tggccttgta
      301 cgcggaggcc gcttctcctg gttctaatag agccacgtcg gttcccctca gaggtgccca
      361 ccgaacgctg attagaccgc ccgtgggggc atttttcgac ttcaaccatc ttttaccgcc
      421 agtggcggcc caacacatcc aaagccgact tttaccgcca cccgcgacaa aattcaacac
      481 ccggcggcgc gtgagcgcct ttcaagccgg cgactttgtc aaatcagtgt gggccattca
      541 gaggtgaggt ggttagacag gcgcagccct cggcgcggca gacgaaaggc acgcgagaca
      601 aaggagcggt gccagcccgc cgaacgttct accctttcag ctctggagcg taggctcacc
      661 gtccgcctcc tctccgtaag ccttagccaa ctgcgccagc gatccggcgg gcacttccac
      721 gtttatcagg tagccgcccc cggcgtccgc cgtgatttgc acgacctcgc gcggagcttc
      781 gtccaagcac gcgcgctcta gcctctccag tcggcttcct ctatttacct gcggccccca
      841 cgcttctgcg cctccagcgc gtcctcgatg gcctttagcc gcacgccaag ctccgcctgc
      901 tcatgaatct ttagcgccga ttcaatgatg atgcgcgcgg cggccacgcg cacgctggcg
      961 ggcttcgatt cgtcctcggc gatgtcggag aggatgcgcg ccgcgccttc cgctttcgcc
     1021 tggagccgcg tctgtaggtt gtccatgagg ccgcggcggg cctggcgcac acgctcggcg
     1081 aaagcaggct cgcgcaggag tgtaggcccg cggcccgttc cagcttcccg actctgttcg
     1141 ttaggcgcat ctcttacggc gccgaacgtc ggcgacgccc tcccgcagaa aagtcttgac
     1201 gggcgcgccg ccggtcatta cattcatgga agagccttcg gcccccgaag gccgcacgcc
     1261 acggccccgc gcctccgccg atttccgacc ttaaccgcct cttgtgaacc catcatggcc
     1321 gaactgctca aagtcgccct gctgtgctgc ctgatcgtcg gggtcgctgc cctgagcgcg
     1381 ctggccgccg tggccctgcc gctctgacac gcgcccgcct ggcagtgcgc gggcggtcgt
     1441 ccttcgggtc gagcgtcgcc cctgcctcgt cttactgagg taggcgtatg gttcgccagc
     1501 ggacacacca ttaacacaat cgtgtcgccc cgcggggtag aatccgacct tgccgctttc
     1561 cgcccacgcg cctaaaatcc gcccatgaca agcaataccg tcatggttat ttttgccctg
     1621 ttcgtgctgg tactgttcct actcctgctg actctcatgc tggggctcag gcggatgaag
     1681 tagaaaccgc gccgtgaagg acgtagactt taagctggcc tgcgccgtcg ccgatttcgg
     1741 cagagcgcgc ccatcaccgc ggggagacgg caagtcgctc tcaactaccc ctctcaaccc
     1801 cttccgcgcc ccatatccgg tgcccatatc cgacatcggg caagggcttg ataacacccg
     1861 cttatcgccc tcataaaccg ccggtttttc tccattaccg tagcccgcac gcggcacgca
     1921 ctttgtaaga gtcgggagac accttgaacc cctcgacgcc gtgcccgccg caccgggtac
     1981 gtgaggcgcc ggggctttgt gtgtcggggt gggggttcgc ggcttctggg gagaagggtg
     2041 cgaaccccca cccgttttct tgtccactta gcgccataaa gcaaaacagc cgcccgcgtg
     2101 tgtaccgtgg tacacatcgc aaggcggcca cgtcgtaagc gttaccgtga taacgctcgc
     2161 cggtagggcc gattaagttt tagcgccgcg gggctgcttc tcctcaagct cggcgtggag
     2221 cacgccgccc gcgtgggcgt gcagtataac ccgcccgctc ggcgcgtggc cgaaaatctc
     2281 acgcaggaga cgcgcgagcc tttcgcgcgt ctcggcgtcg agtgtggcgc gcgtgcgccg
     2341 gatcgtctcg acttctcttt tcacgccgcc ccgcccctct caccgcgcgc gtagttccgc
     2401 accgtgcgct gcaccaaatc ccatatatcc ctgtccgaag gcggtgggtc tgcggggtcg
     2461 tgctcacagc gggtgtctct gacttcgagc acctgccggt agaggtctga agcgtcttcg
     2521 gcgtagccgt gcgtccagcg tcctaacatc acgtcgcgca gcccgtcgtt gcgctcacgc
     2581 ttaccgaaga agcgtgcgcc gcccgtggac gttcgccgcc ggtcgcggta gtcctgaaag
     2641 tcaatcggcg gtggcggtga ctcgcctgcg gcggccttct tcagcgtctc cacgatccac
     2701 tccggggcat ccgcgacggg cgaatcagtc tcgaagccgt aggggcggcc cgagacgtga
     2761 gacgagccgg gcgcgatgat gtacccgccc gcggccttca cgtccacgcc ggggccgagc
     2821 ttgcacttca gctcgccctt cgccctcttg atcgtttcgg gcagtctgta tttcttgtgc
     2881 cggccgccgc cgccggtttt cttctcgcgc gtcggcgtaa actcgccgtg cgcctcttcc
     2941 ggatcgaagt acgtcgcgtc gccgccgttg cgcgggtcaa cgtcgatgcg aatcacttcg
     3001 cccatcgcct cgccgaagtt gatgcgcggg tctatagcgt gccagcggcg gaatgtggcc
     3061 gcgtccgtcg tcgcgctgta aacgccgttc gggcagaact tcggataggg atgcttccca
     3121 ggactcggac actccgaacc cttcgggcaa tcacaaatcc cgtcgcagac gccccacacg
     3181 gcgagaatcg gcgtcccttc cgcagctcgc ggccattggc atccattcat acccctctaa
     3241 cttaaatgtg tgttaagaga ggtgggatgt gaacaggtgg tgttatcgaa gagttacaag
     3301 accggcccgc cctcgcccgt catggacatc ctgtgcgggc ggcagtcaca cccgcgccga
     3361 gtcaacgacc tactgttcgc ggagaagggg ggcggagcga gaaaggcacc gcagaaaagc
     3421 cccgccgccg cgctgaagcc cgaagaggcg gttggaagcc cgccacacta tgtcgtgcct
     3481 ggcagcttcg actattctcc gccacgccgg gcgttcggtg ctaatcttcg acgatggggg
     3541 gatgaatcca catgcgaaag aatctttcga gagcctcttt gcttctcctc gctctcgcga
     3601 ccctactgct cgccgcgggc gtcgcggcgg cgcaggaaga gacgacgctc aaggacacga
     3661 cgcccaagag gcgtttgcgg agtccggcgt cggcgcgggg cttcatcggc ggcgagtcgc
     3721 acgacagcta cgtcgtccgc gcccgcaggg ggcagaccct gaccgtgcga atctcgtggc
     3781 ggcgcgaggg cgacaaccgg gcggagttca gcgtgagcga gtcgccggac ttctacaccg
     3841 ccgagccggc ggagttcggt gcggcgtccg gcgggcggtg gtcgggcaag gtgccgcgca
     3901 gtggtgatta ttatatctac gtggtggcgc acccgacggc gcgctacacg ctcagggtgc
     3961 gggtcgagta gccgccggaa gaggggaagg gaagaggcgg gttggaggca cgcccctcct
     4021 gttttcgtga ctattcccgg cgtgtgggga tatcatcggg cgcgccgcag acgccgggcg
     4081 cgaggccgtc cagatgtttg tcgtgcaggt gtggcgatga ggaatcggac tttcagcagg
     4141 gggcacttgt ttgtttgtac cgtggcgggg gtggccctgc tctgcttcgg ctggacggcc
     4201 taccggcggg ccgaggagca gtccgtgctg cgtacccact ccggcctgtt ggcgctgctc
     4261 gtcgcgggcg attacgagcg ggcttaccga ctcaccacgg acgagtaccg ggccgctcac
     4321 acggtcgggg agtttcaggc acgcttccgc cactatgaag gagagaagca ttacttgaaa
     4381 cgaagcccga ccgtgctctc gttgggcctg cgttcggcgg aggtttacgc cgacgaagtc
     4441 cgcagcctca tcgagttcct caacgggccg tcattctttt accggaagga gcgagggcag
     4501 tggcgcttca ccggcgaggt ggcgcactac atggactaac ggccttacgc cgcggcgcgg
     4561 ggttgctccg gcgtgatact caccagcgcc aaccgctcgc gccggcccac cctctgctcg
     4621 ccgtgcgcgg ccagcacgac ctgcacgcgc agcgccccgc cgtcgcgggg gacgctcagc
     4681 gcgaacctct cctgttgctg cccgccgtcc atgaaggcga ggaagcgggc gcggaagccg
     4741 tacagttgct cggcgggcat gacctcggtg aagaagtcgc gccccacgac gcgctcgcgc
     4801 cgcaggctcg cgcggggcgt cggggcgtag cggataacga ttcccgtcgg gtcgagttca
     4861 agcagaccca gcggcagcgc gtcggtcgtg tgctgaccgt tcatagtttc gatacctccg
     4921 gcggacggtt attggctggg gcggttggtg gttcaaggga gcagggagtt ttatgtggaa
     4981 gagtgggcga acgacagcat acgcctgaac aggcgggggc tgtggggggc gcggccctcc
     5041 tgcgtctccc tcgcggtgat gacttccgcc gcggcgaagg ccctgacgac ctcctccggc
     5101 cagcgacggt gggcgtagca ttcccccctg gtggcgagcg acacctgaac catctcttcg
     5161 gtcgtaagcg gcttcaacat gatgcactcc ttcgattgat ccggtcgccg ggtgggtggt
     5221 gggcgtaacc ga
//
LOCUS       DBNGOH010000041         3393 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1106137, whole genome shotgun sequence.
ACCESSION   DBNGOH010000041 DBNGOH010000000
VERSION     DBNGOH010000041.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3393)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3393)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3393
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1106137"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..409)
                     /locus_tag="AC159P_00840"
     CDS             complement(<1..409)
                     /locus_tag="AC159P_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_463674459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(167..169),aa:Sec)
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HIR3694024.1"
                     /translation="MARIEGVEEGRVDGYIAAVFKAQAKKWGAPLLNHLVYARRASIF
                     RGARAMWQGIDASGLIDARLQALLNRRVASLNGCEFUQDINAAVGSELGVAEEKIDQL
                     ASYATSPLYDEADRAALAYADAMTVTGRDVTDAL"
     gene            683..955
                     /locus_tag="AC159P_00845"
     CDS             683..955
                     /locus_tag="AC159P_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013322453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694025.1"
                     /translation="MSRQISDDSQGDTGTPDITYNLISTIYHTLQGAETQAMYIADAE
                     QTGDQELIQFFRDIKEENQRRADRAKQLLARFVGQDQKQGQAASGS"
     gene            complement(1079..1399)
                     /locus_tag="AC159P_00850"
     CDS             complement(1079..1399)
                     /locus_tag="AC159P_00850"
                     /inference="COORDINATES: protein motif:HMM:NF012790.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HIR3694026.1"
                     /translation="MEEITATELKQRLDRGDDIQLIDVREPGEYEVARIPDAKLIPLG
                     EVASRKDEIEEGRETVVHCKGGVRSARAIEALTRAGFKGKLINLKGGITAWSNDVDPS
                     VPKY"
     gene            complement(1465..2883)
                     /locus_tag="AC159P_00855"
     CDS             complement(1465..2883)
                     /locus_tag="AC159P_00855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694027.1"
                     /translation="MRRTHTGGLLFFTCAALLASLSSPHARAQEVAAAPAQEENVEDA
                     PWQARKLDEFGQVRGCDHGARLDNLATELLNRPDLTAYVVAYGPSGEGSGTADFRLQV
                     TRDYLVMTRGLLADRIKLVNGGAYKDKHQSWVELWVAPPGAEPPPPPRKFENDAATFA
                     GLYSEFETWDISGMTGEALGPPVGNSNLAGFAEVLRLQPSARAYIVAFNGEESAFHAW
                     RRVADREIEQLKRRYGVKDDRVSVIFGGQEKELKLQLWVLPPDAPPPAEDAGRERRPA
                     KATMLGPFEGYLLKYPDEARTFFKAFAEALKADEEMTISLAVYLPSSSSPEEEEATEE
                     SGESPREEESDESPPATDAATDAAPDPEEPPEVDLVQLVEKWRGDLVKEYGIGGQRLV
                     VTFVELEGDVWSSGSISAWLVPPGVLPPDPLAEEEDGTETETEEAASAEGAKPAEADE
                     AKPVPAEHAEEPWKEPPTAPRR"
     gene            complement(2952..>3393)
                     /locus_tag="AC159P_00860"
     CDS             complement(2952..>3393)
                     /locus_tag="AC159P_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014101327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HIR3694028.1"
                     /translation="AQLIGLSVDSVTAHIAWIRNIEQHFDVKVDFPVIADLDTKVAQK
                     FGLIHPAASETATVRAVFLIDPQQKVRALIYYPMSCGRNIDEVLRALDALQTADANAC
                     ATPANWQVGEKVIIPPPQTLEDAEKRVNTPDIEVTDWYFSKRSL"
BASE COUNT          499 a         1165 c         1097 g          632 t
ORIGIN      
        1 acaatgcgtc ggtcacgtcg cgcccggtga cggtcatcgc gtcggcgtat gcgagggcgg
       61 cgcgatccgc ctcgtcgtag agcgggctgg tcgcgtacga cgccagttgg tctatcttct
      121 cctccgcgac gccgagttcg ctgcccacgg cagcgttgat gtcctgtcaa aactcgcagc
      181 cgttgagcga cgcgacgcgc cggttcagga gcgcctggag ccgcgcgtcg atcagccccg
      241 acgcgtcgat cccctgccac atcgcacgcg cgccgcggaa gatggaggcc cgccgcgcgt
      301 aaacgaggtg gttcagcagc ggcgcgcccc acttcttcgc ctgcgccttg aagacggcgg
      361 cgatgtagcc gtccaccctt ccctcttcga ccccttcgat gcgtgccatg tgtctcctct
      421 ccgacctcgc gccgtgatgg atttgcgccc gacgcccggg atgttaacgc gcccgcggct
      481 tcgggcaagg gccatttacg agaacgccct gatgccgttt ggcgggtaag acctctatca
      541 ttgcttcgaa gtcggcgggc ttcgaaactg cccgcggtcg cgccttcttg acccgcgtcc
      601 gcacagttct tacaatcaca ggcgatcccc cttcgtggcc ccctccgtaa tctcgacgac
      661 attctcgtaa aggggtatat ccatgtccag acagatcagc gatgacagcc agggtgacac
      721 cggcactccc gacatcacct acaacctgat cagcaccatc tatcacactc tacagggcgc
      781 tgagacgcag gccatgtaca tcgccgacgc cgagcagacc ggcgaccagg aactcatcca
      841 gttcttccgc gacatcaagg aagagaacca gcggcgcgcg gaccgcgcca agcaactgct
      901 cgcgcgcttc gtcggccagg atcagaagca ggggcaggcc gcgtccggct cctgaagccg
      961 cgcgcaatga tgggaagacc gtgatttaaa gagtgaacgc ctgccgcaga cacggcaggc
     1021 gttcactctt ttcgtggttg tacgcgggcg gcgcgggccg tacagcctcc cgcgccgctc
     1081 aatacttcgg gacggaaggg tctacgtcgt tcgaccaggc ggtgatgccg cccttgaggt
     1141 tgatgagctt gcccttgaag cccgcgcgcg tgagcgcctc gatggcgcgg gcgctgcgga
     1201 cgccgccctt gcagtggacg accgtctcgc gcccctcctc gatctcgtcc ttgcggctcg
     1261 cgacttcgcc gagcgggatg agcttcgcgt cggggatgcg cgcgacctcg tactcgccgg
     1321 gttcgcgcac gtcgatgagc tggatatcgt cgccccggtc gaggcgctgt ttcagttcgg
     1381 tggcggtgat ctcttccata cgattttctg tctcccgttc tcgtctgttt cgaatcgctg
     1441 tctgacacct gtcggccttc ggcctcaacg ccgcggcgcc gtcggcggtt ccttccacgg
     1501 ctcttccgcg tgctccgccg ggacgggctt cgcctcatcc gcctcggcag gcttcgcgcc
     1561 ttcggcggag gccgcttcct cggtttcggt ctccgtcccg tcttcctcct cggccagcgg
     1621 gtcgggcggc aggacgccgg gcggcacgag ccacgcggag atcgaacccg aactccaaac
     1681 atcgccctca agctcgacga aggtcacgac gagccgttgc ccgccgatgc cgtattcttt
     1741 gacgaggtcg cccctccact tctcgacgag ctgcacgaga tcaacctcgg gcggctcttc
     1801 ggggtcgggc gccgcatcgg tcgccgcgtc ggtcgcgggc ggcgactcgt ccgactcttc
     1861 ctccctcggc gactcgcccg actcttccgt cgcttcctct tcctccggcg acgatgacga
     1921 cgggaggtag acggcgagag aaatcgtcat ctcctcgtct gccttcagcg cctccgcgaa
     1981 cgccttgaag aaggtgcgcg cctcgtccgg atacttcaac aggtatcctt cgaacgggcc
     2041 gagcatcgtc gccttcgcgg gtctccgctc gcgcccggcg tcttccgcgg gcggcggcgc
     2101 gtccggcggc agcacccaga gctgcaactt cagctctttt tcctgaccgc cgaagatgac
     2161 gctcacgcgg tcgtctttga cgccgtaccg ccgcttgagt tgttcgatct cgcggtcggc
     2221 gacgcggcgc cacgcgtgga aggcgctctc ctcgccattg aaggcgacga tgtaggcgcg
     2281 cgcggacggt tgcagccgca acacctccgc gaagcccgcg aggttcgagt tgccgacggg
     2341 cgggccgagg gcctcgccgg tcatgccgct tatgtcccac gtctcgaact cgctgtacag
     2401 gcccgcgaag gtggccgcgt cgttctcgaa cttccgcggc ggcggcggcg gctccgcgcc
     2461 gggcggggcg acccacagct cgacccacga ctgatgcttg tctttgtaag cgccgccgtt
     2521 gacgagcttg atgcggtctg cgaggaggcc gcgggtcatg acgagatagt ccctcgtcac
     2581 ctgaagccgg aagtctgcgg tgccgctccc ctcgcccgag gggccgtaag cgacgacgta
     2641 ggccgtcagg tcgggcctgt tcaggagttc ggtggcgagg ttgtcgagcc gcgcgccgtg
     2701 gtcgcagccg cgcacttgcc cgaactcgtc gagcttgcgc gcctgccacg gcgcgtcctc
     2761 gacgttctcc tcctgcgcgg gcgcggcggc gacctcctgt gcccgcgcgt gtggagacga
     2821 gagcgacgcg agcagcgccg cgcaagtgaa gaacagaagg ccgccggtgt gcgttcgtct
     2881 caacgtcgtg cgcacctttc cgaaaacgga cctttagcgc ccggctctgc cgggaacgag
     2941 atgctcgtcg gttacaggct gcgcttcgag aagtaccagt cggtgacttc gatgtcgggc
     3001 gtgttgacgc gcttctcggc gtcttccagg gtctgcggcg gcgggatgat gaccttctcg
     3061 ccgacctgcc agttggcggg cgtggcgcag gcgttggcgt ccgccgtctg caaggcgtcg
     3121 agcgcgcgca gcacctcgtc gatgttgcgc ccgcacgaca tcgggtagta gatgagcgcg
     3181 cgcaccttct gctgcgggtc gatgaggaag acggcgcgca cggtcgccgt ctcggaggcc
     3241 gccgggtgga tcagcccgaa cttctgcgcg accttcgtgt cgaggtcggc gatgaccggg
     3301 aaatcgacct tcacgtcgaa gtgctgctcg atgttcctga tccacgcgat gtgcgccgtg
     3361 acggaatcga ccgagaggcc gatcagttgc gcg
//
LOCUS       DBNGOH010000042         2753 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1656411, whole genome shotgun sequence.
ACCESSION   DBNGOH010000042 DBNGOH010000000
VERSION     DBNGOH010000042.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2753)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2753)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2753
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1656411"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..567
                     /locus_tag="AC159P_00865"
     CDS             <1..567
                     /locus_tag="AC159P_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011554359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HIR3694029.1"
                     /translation="GPLFGIQSWVALPLRAEDAAPAFAHHGAGELPLIEDKGKRVRLI
                     SGALYGARSPVETLSEMFYADASLEAGARLPLPTEHEERAAYVVEGEVEIDRDGGAYG
                     AGQLVVFKPRASVTLGAKGSAPARLMLLGGEPLEGRRHIWWNFVSSSGERIEQAKQDW
                     KAGRFAPVPEETERIPLPESGPVVVRYP"
     gene            complement(849..1634)
                     /locus_tag="AC159P_00870"
     CDS             complement(849..1634)
                     /locus_tag="AC159P_00870"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018785618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR3694030.1"
                     /translation="MRVLITGGTGSLGRALVRAVAGAGHVARVMSRRERADTAGDGVE
                     WARADIATGDGVRAAVEGADAVIHAATDPRRSEVVDVRGTRHLVEAARAAGVGHFVYV
                     SIVGIDRIPLGYYRSKLAAERIVTESGLPHTTLRATQFHPLVAGFISKAARVPLLLPL
                     PTDFKFQCVAEAEVAARLVGCLDEGPRGRAEHFGGPEVLTLGEMAVGWMEAKGVRKKL
                     VPLPLPGAAAAAFRAGKNTAPHGARGSIRWRDWLADSSTVDER"
     gene            complement(1659..2075)
                     /locus_tag="AC159P_00875"
     CDS             complement(1659..2075)
                     /locus_tag="AC159P_00875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694031.1"
                     /translation="MSAALSRSSNTLARLSLAVVVAHALIGVVHSAAHVVMHIFMSLW
                     QNAYIFAVIFALPIVAALLLRRRARAGYLLLFLSMLGSLLFGGYYHFVAPGPDNVNSM
                     GGHAWASPFRGSAVLLALTEAAGAFLGLVGLLDRRR"
     gene            complement(2149..>2753)
                     /locus_tag="AC159P_00880"
     CDS             complement(2149..>2753)
                     /locus_tag="AC159P_00880"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009196619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HIR3694032.1"
                     /translation="RTRKRNEPGAAYEYNDVRVNALALAALNVWRRPLPQVLREYVME
                     PIGASTTWRWYGYETSWVEIDGEAVQSVSGGGHWGGGMFINARDMARFGLLTLRRGRW
                     KDRQILSEEWLRAATTPTPVRPGYGFMNFFLNTERKFLPSAPAAAFAHVGNGTNMIYV
                     DPENDLVVVIRWIENEAIDGFIKRLLASINAPAPESKGRD"
BASE COUNT          462 a          920 c          933 g          438 t
ORIGIN      
        1 ggcccgctct tcggcatcca gtcgtgggtc gcgctcccgc tgcgcgcgga ggatgccgcc
       61 cccgccttcg cgcatcacgg cgcgggagag ctgccgctca tcgaagataa aggcaagcgc
      121 gtgcgtctca tcagcggcgc gctctacggc gcgcgctcgc cggtcgagac gctctcggag
      181 atgttctacg cggacgcctc actggaagcg ggggcgcgac tccccctgcc gacggagcac
      241 gaggagcgcg ccgcctacgt cgtcgaaggg gaggtcgaga tagaccgcga cggcggcgcc
      301 tacggcgcgg ggcaactcgt cgtcttcaag ccgcgcgcgt ccgtcacgct cggcgcgaag
      361 ggttctgcgc ccgcgcgcct catgctcctc ggcggcgagc cgttggaggg gcgccgtcac
      421 atctggtgga acttcgtgtc gagttcgggc gagcgcatcg agcaggcgaa gcaagactgg
      481 aaggcgggcc gcttcgcgcc cgtgcccgaa gagacggaac gcatcccgct gcccgagagc
      541 gggccggtcg tcgtccgtta cccttgaagc aagggacgaa ggatttcgga tttcggattt
      601 ccgttgacga ttgatgacag cgccgctctc atcaatcgtc agtgctcaac gagcgccaga
      661 gcgaatcgcc gtcgagtgta gcgtgcgctc atctcaacgc agagaacaca gagaggcgca
      721 gagggcgcag agaagaaatt gtttttctct gcgccctccg cgcctctgcg tcctccgcgt
      781 taaatcctgc cgctagctaa actcaaccgc aaactgctct aatccacaat ccacaatcct
      841 tcgtcccttt acctctcgtc cactgttgaa gaatccgcga gccagtcgcg ccagcgaatc
      901 gagccgcgcg cgccgtgcgg ggccgtgttt ttgccggcgc ggaaggccgc cgccgccgct
      961 ccgggcagag gcaggggcac caactttttc cgcacgccct tcgcttccat ccaaccgacg
     1021 gccatctcgc cgagggtgag cacttcgggg ccgccgaagt gctcggcgcg cccgcgcggg
     1081 ccttcgtcca gacagccgac gaggcgcgcg gccacttccg cctccgcgac gcactggaac
     1141 ttgaagtcgg tcggcagcgg cagcaggagc gggacgcgcg cggccttcga gatgaacccg
     1201 gcgacgagcg ggtggaactg cgtggcgcgc aaagtcgtgt gcggcaggcc ggactccgtg
     1261 acgatgcgct cggcggcgag cttgcttctg tagtagccga ggggaatccg gtcgatgccg
     1321 acgatggaga cgtagacgaa gtggccgacg cccgcggcgc gcgcggcctc gacgagatgt
     1381 cgcgtgcctc gaacgtccac cacctccgag cgtcgcgggt cggtggcggc gtggatgaca
     1441 gcgtccgccc cttccaccgc cgcgcgcacg ccgtcgcccg tggcgatgtc ggcgcgcgcc
     1501 cactcgacac cgtcacccgc ggtgtcggcg cgctcccgcc gactcatgac gcgcgcgacg
     1561 tgccctgccc cggcgacggc gcgcacgagc gcccgaccga gtgatcctgt gccgcccgtg
     1621 atgagaactc tcattgtctt cgatcgaaga agaacgttct atcgtcgccg gtcgaggagg
     1681 ccgacgaggc cgaggaaagc gcctgccgcc tccgtgagcg cgagcagcac cgccgagccg
     1741 cggaaggggg aagcccacgc gtggccgccc atcgagttga cgttgtcggg gccgggcgcg
     1801 acgaagtggt agtagccgcc gaagagcagc gagccgagca tcgacaggaa gagcagcagg
     1861 tatccggccc gcgcgcggcg tcggagcagc agagcggcga cgataggcag cgcgaagatg
     1921 acggcgaaga tgtacgcgtt ctgccagagc gacatgaaga tgtgcatgac gacgtgcgcc
     1981 gccgagtgga cgacgccgat cagcgcgtgc gcgacgacga cggcgaggct cagccgcgcg
     2041 agcgtgttgc tgcttcttga gagtgccgct gacatacgtc tcgacgccaa cctccttcaa
     2101 acacgtccgc tgtcattaac cgccggtcac gactcccttc gtcaaccgct aatcgcgccc
     2161 tttcgattcg ggcgcgggcg cgttgattga cgcgagcagg cgcttgatga agccgtcgat
     2221 ggcttcgttc tcgatccagc ggatgacgac gacgaggtcg ttctcggggt ctacgtaaat
     2281 catgttcgtc ccgttgccga cgtgggcgaa ggctgcggcg ggcgcgctcg gcaggaactt
     2341 gcgctcggtg ttgaggaaga agttcatgaa gccgtagccg ggccggacgg gcgtgggcgt
     2401 cgtcgccgcg cgcagccact cttcggagag aatctgtctg tctttccaac gaccgcgccg
     2461 gagcgtcagg aggccgaagc gcgccatgtc gcgggcgttg atgaacatgc cgccgcccca
     2521 gtggccgccg ccgctgaccg actgcacggc ctcgccgtcg atctccaccc aagaagtttc
     2581 atagccgtac cagcgccacg tcgttgacgc gccgatgggt tccatcacgt attccctgag
     2641 cacttgcggc agaggccgtc gccagacgtt gagcgcggct aaagccaacg cgttcacgcg
     2701 cacgtcgtta tattcgtaag cggcgcccgg ctcgttgcgt ttgcgcgtgc gcc
//
LOCUS       DBNGOH010000043         5097 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4222562, whole genome shotgun sequence.
ACCESSION   DBNGOH010000043 DBNGOH010000000
VERSION     DBNGOH010000043.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5097)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5097)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5097
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4222562"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(81..1043)
                     /locus_tag="AC159P_00885"
     CDS             complement(81..1043)
                     /locus_tag="AC159P_00885"
                     /inference="COORDINATES: protein motif:HMM:NF012694.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HIR3694033.1"
                     /translation="MLPFLAFIFCTFLVFALYLLATRGTEAKRKRLQQRLAETLVYSS
                     NAEDIEIQLARQELMSEIPFVNRMMVRMQVATRLKRMLDQADLHITVTRLVMFSLLAG
                     ILAAQACSMLTISKLLWVCAGLGAASVPFIHVVYKRRQRLDAFLGLLPDTLELMSRAL
                     SAGHAFPESLHMVATEMPEPIATEFRKTYEEQNLGLSLKLALGNLAERIPLLDLKLCI
                     TAVLIQRETGGNLAEILEKVSYTIRERFRIMEDLKTLTTSSRMSAWILCGLPVFVAIA
                     VTGMNPEYMSVLWRDPRGHNLLIAAAFMQVTGMLIIRKILNIKI"
     gene            complement(1127..2476)
                     /locus_tag="AC159P_00890"
     CDS             complement(1127..2476)
                     /locus_tag="AC159P_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011000609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TadA family conjugal transfer-associated ATPase"
                     /protein_id="HIR3694034.1"
                     /translation="MALRERLVRETGSGVAALYNRGGEEGAQPNQHTFQEMKARLHRA
                     IINRMDLTKLGQLEPEQLHAEVARLAEDLLLIENAPLSANERERLVGEVRHELFGLGP
                     LEPLLADPSVSDILVNSPKNIYIERGGRLERTAVEFKDDEHLMRVIERIVSMVGRRID
                     EASPMVDARLKDGSRVNAIIPPLALDGPVLSIRRFGADPLRMSSLIEKGALTKDIADT
                     FEMCVHARLNILISGGTGAGKTTLLNALSAYIPHEHRIVTIEDSAELQLQQPHVVRLE
                     TRPPNIEGKGEVTQRDLVKNALRMRPDRIVIGEVRGGEAIDMLQAMNTGHDGSLTTVH
                     ANTPRDALARLETMIQMTGMKLSERAMRQQVASALNVVIQVARLSDGTRRITSISELT
                     GMEGDTITMQEIFQFERKGVDKDGKVIGRFRPTGVRPRFAERLKTYGMQLPRVFFEE"
     gene            complement(2572..3813)
                     /locus_tag="AC159P_00895"
     CDS             complement(2572..3813)
                     /locus_tag="AC159P_00895"
                     /inference="COORDINATES: protein motif:HMM:NF013793.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpaE/TadZ family protein"
                     /protein_id="HIR3694035.1"
                     /translation="MTNKLTFVILSHGLENFKELRAAVGAQEGAQVLVGGDDAEQIFP
                     EIERLRPSAAIITLGSQPENDLRFVAQVARECPQTAVICASSDASPDLILRSLRAGAR
                     EFLRLPVSPEEFKTVYERTADLSRVQPTAPKKRGRVVAVFSSKGGCGTTFIAANVAAA
                     IDKPTVLVDLNLQAGDLGLFLGVEPKFSIADIVESRERVDDSLMKSYLAPHSQNLSLL
                     AAPREADSADDIKPEHIYEVLELLRERFDYVVIDPQHTFDAITLAALDQADEILLVLT
                     LDIPAIRSAQRSLQLFERLGYPRHKVSIVVNRFSKQIDLDLQQVERFLGERVTGYVQS
                     DYRTAVNSINLGQPLVESEPASKIAAELRQITAQVTGAQPAVPPETRSGLLGNLFRRK
                     SGDAPRAVLPAAGKEKEPATV"
     gene            complement(3875..4330)
                     /locus_tag="AC159P_00900"
     CDS             complement(3875..4330)
                     /locus_tag="AC159P_00900"
                     /inference="COORDINATES: protein motif:HMM:NF019428.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HIR3694036.1"
                     /translation="MRIKSSKRGERGAALVEFSIGALVFITAVFAVIEFGRLLWTHNA
                     LTDAARRGARYAVNHGTTADDIKNVERMVVYGTTEDGASPLVADLKHANVKVEHSAGF
                     NVGEGTARVSIQNYDFNFVVPLIGKKVRMPAYTTALTGENAGQVPVALP"
     gene            complement(4341..4850)
                     /locus_tag="AC159P_00905"
     CDS             complement(4341..4850)
                     /locus_tag="AC159P_00905"
                     /inference="COORDINATES: protein motif:HMM:NF019428.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HIR3694037.1"
                     /translation="MRNLRNGIVRRLRLFGASERGVQLIELAIALPVLLMLFAATAEF
                     GRYFYHYSTLAKATRAGARYLATTPVKKTGGNALEDPKVKNLVVYGDPNAGDGGTPII
                     PGLTPANVEIERDGPIITVPDTVTVRIINYNYTPMIDLGDFTKGKSWTAVPLKPSTTM
                     RYLLTQPTP"
     gene            complement(4861..>5097)
                     /locus_tag="AC159P_00910"
     CDS             complement(4861..>5097)
                     /locus_tag="AC159P_00910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694038.1"
                     /translation="GRRIVIIPIVLQSEYDPGRNVVRFNRFGLFFLRKKVGGGSGGEL
                     EAEYIDDPTIVGRGGVGGGTAAANGLLAKPVLYQ"
BASE COUNT          786 a         1583 c         1765 g          963 t
ORIGIN      
        1 taagcagttg tcgccgagcg gtgatgcgag tcgagcagct tcttgcttgg tactgctcac
       61 tgctcaccgc tcacggctca ctaaatcttg atgttcaaaa tcttccgaat gatgagcatg
      121 ccggtgacct gcatgaaggc tgcggcgata aggaggttgt ggccgcgcgg gtcgcgccac
      181 aggacgctca tgtactcggg gttcatcccc gtgacggcga tggcgacgaa gacgggcagg
      241 ccgcacagaa tccaggcgga catgcgcgag cttgtggtca gggttttcag gtcttccatg
      301 atccggaagc gctcgcggat cgtgtaggac accttctcca ggatctccgc gaggttgccg
      361 cccgtctcgc gctggatgag gaccgccgtg atgcacagct tgaggtcgag gagcgggatg
      421 cgctccgcga ggttgccgag cgcgagcttc agtgagaggc cgaggttctg ctcctcgtag
      481 gtcttgcgga attcggtcgc gataggctcg ggcatctcgg tggcgaccat gtgcagcgac
      541 tcggggaagg cgtgccccgc ggagagcgcg cggctcatta actcgagcgt gtcgggcagc
      601 agcccgagga acgcgtcgag gcgctgccgg cgtttataca cgacgtggat gaaaggcacg
      661 gacgccgccc ccaggccggc acacacccac aagagcttcg agatggtgag catcgagcag
      721 gcctgtgccg ccagaatgcc ggccagcagg gaaaacatca cgagccgcgt gaccgtgata
      781 tgcagatcgg cctggtcgag catgcgcttg agccgcgtcg ccacctgcat gcgcaccatc
      841 atccggttga cgaacgggat ttcgctcatc aactcctgac gcgcgagctg aatctcaatg
      901 tcttcggcgt tcgacgagta aactaacgtc tcggccagcc gctgttggag ccgcttgcgc
      961 tttgcctccg tcccacgcgt cgccaggagg tagagcgcga agaccaggaa cgtgcagaat
     1021 atgaaagcca ggaatgggag catcgttttt ttccagtgcg cagttccgtt atttagcgag
     1081 cagcgttcgg cgcagcttgt tccgcccgct gccctccgca tcccgcttac tcttcaaaaa
     1141 acacccgcgg cagttgcatg ccgtaagtct tcagcctctc ggcgaagcgc gggcgcaccc
     1201 ccgtcgggcg gaagcgtccg atgaccttgc cgtccttgtc gacgcccttg cgctcgaact
     1261 ggaagatctc ctgcatcgtg atcgtgtcgc cctccatgcc cgtaagctcc gagatggagg
     1321 tgatgcggcg cgtcccgtcc gacagtcgcg caacctggat caccacgttg agggccgagg
     1381 cgacctgctg gcgcatcgcc cgctccgaca gcttcatccc ggtcatctgg atcatcgtct
     1441 ccaggcgggc gagcgcgtcg cgcggggtgt tggcgtggac ggtcgtcaag ctcccgtcgt
     1501 ggccggtgtt catcgcctgc aacatgtcga tggcctcgcc gccgcgcacc tcgccgatga
     1561 cgatccggtc ggggcgcatg cggagcgcgt tcttgacgag gtcgcgctgc gtgacctcgc
     1621 ccttgccctc gatgttgggc gggcgcgtct ccaggcggac gacgtgcggc tgctggagct
     1681 gcagctccgc cgagtcctca atcgtcacga tgcgatgctc gtgcgggatg taggcggaga
     1741 gggcgttgag gagcgtcgtc ttgccggcgc cggtgccgcc cgagatgagg atgttgaggc
     1801 gcgcgtggac gcacatctcg aacgtgtcgg cgatgtcttt ggtgagcgcg cccttctcga
     1861 tgagcgacga catgcggagc gggtcggcgc cgaagcggcg gatcgaaagc acggggccgt
     1921 cgagcgcgag cggggggatg atggcgttga cgcgcgagcc gtccttgagc cgcgcgtcca
     1981 ccatcggcga cgcctcgtcg atgcggcggc ccaccatcga gacgatgcgc tcgatgacgc
     2041 gcatcaggtg ctcgtcgtcc ttaaactcca cggcggtgcg ttcgagcctg ccgccgcgct
     2101 cgatgtagat gttcttcggc gagttgacga ggatgtcgga gaccgacggg tccgccagca
     2161 gcggctccag cggccctagg ccgaacagct cgtggcggac ttcgcccacg aggcgctcgc
     2221 gctcgttcgc cgagagcggc gcgttctcga tgagcagcag gtcttcggcc aggcgcgcca
     2281 cctcggcgtg gagttgctcc ggctcgagct ggccgagctt ggtcaagtcc atgcggttga
     2341 tgatcgcgcg gtgcagccgc gccttcatct cctggaaggt gtgctggttg ggctgcgcgc
     2401 cttcttcgcc gccgcgattg tatagcgccg cgacgcccga acccgtctcc ctgactagcc
     2461 gttcacgaag tgccatgatt gatgtgtgcc ccgtcggtgt ttgatgagag cttgtttgtt
     2521 cggaatctca gctcggagat tcgagcgttg acgcccctgt ggcctgacgt actaaacggt
     2581 cgccggctct ttctctttgc cggcggcggg caggacggcc cgcggcgcgt cgccggattt
     2641 gcggcggaag aggttgccga gcaggccgct gcgcgtctcc ggcggcacgg cgggctgtgc
     2701 ccccgtgacc tgtgccgtga tctgccgcaa ctcggcggcg atcttcgagg cgggttcgga
     2761 ctcgacgagc ggctggccga ggttgatcga gttgacggcg gtgcggtagt cgctctggac
     2821 gtagccggtg acgcgctcgc cgaggaatcg ctcgacctgc tggaggtcga ggtcgatctg
     2881 cttgctgaag cggttgacga cgatgctgac tttgtggcgc gggtagccga ggcgctcgaa
     2941 gagttggagc gagcgctgcg cgctgcggat ggccgggatg tcgagcgtga ggacgagcag
     3001 gatttcgtcg gcctggtcga gcgccgcgag cgtgatcgcg tcgaaggtgt gctgcgggtc
     3061 gatgacgacg tagtcgaagc gctcgcgcag gagttcgagc acctcgtaga tgtgctcggg
     3121 cttgatgtcg tccgccgagt cggcctcgcg cggcgcggcg agcagcgaga ggttttgcga
     3181 gtgcggcgcg aggtagctct tcatcaggga gtcgtccacg cgctcgcggc tctcgacgat
     3241 gtcggcgatg gagaacttcg gctcgacgcc gaggaagagg ccgaggtcgc cggcctgcaa
     3301 gttgagatcg acgagcacgg tcggcttgtc gatggcggcg gcgacgttgg cggcgatgaa
     3361 ggtcgtgccg catccgccct tgctcgaaaa gacggcgacg actctcccgc gcttcttggg
     3421 cgccgtgggc tgcacgcggc tcaagtccgc cgtgcgctcg taaacggtct tgaactcttc
     3481 cgggctgacg ggcaggcgca ggaactcgcg cgccccggcg cggagcgagc ggaggatgag
     3541 gtcgggcgag gcgtcggacg aggcgcagat gaccgccgtc tgcgggcact cgcgcgcgac
     3601 ctgcgcgacg aagcgcaggt cgttctcggg ttgagagccg agcgtgatga tggccgcgga
     3661 ggggcgcagg cgctcgatct cggggaagat ctgttcggcg tcgtcaccgc ccacgagcac
     3721 ctgcgcgccc tcctgcgccc ccaccgccgc gcgcagctcc ttgaagttct ccaagccgtg
     3781 gctcaggatt acgaaagtca gtttgttcgt cattggtttc tcgaccgcgg cccgcccgct
     3841 tgtgagttat gaaccggcgc cccgtacgga gtattcaggg gagggcgacc ggcacctgcc
     3901 ccgcgttctc gcccgtcagc gccgtggtgt aggcgggcat ccgtaccttt ttcccgatga
     3961 gcggcacgac gaagttgaag tcgtaattct ggatgctcac cctggccgtg ccctcgccga
     4021 cgttgaagcc cgccgaatgc tccaccttca cgttcgcgtg cttgaggtcg gccacgagcg
     4081 gggacgcgcc gtcctccgtc gtgccgtaga cgaccatccg ctcgacgttt tttatgtcgt
     4141 cggctgtcgt cccgtggttg acggcgtagc gcgcgccgcg ccgcgccgcg tccgtgagcg
     4201 cgttgtgcgt ccagagcagg cgaccgaact cgatgacggc gaagacggcg gtgatgaaga
     4261 cgagcgcccc gattgagaac tccacaagcg ccgccccgcg ctcgcccctc tttgaagact
     4321 tgatgcgcat cggtggctcc ttacggtgta ggctgcgtca gcaggtatcg catcgtcgtg
     4381 ctgggtttca gcgggacggc tgtccaggac tttcctttgg tgaagtcccc gaggtcgatc
     4441 atcggcgtgt agttgtagtt gatgatcctc accgtcaccg tgtcggggac ggtgatgatg
     4501 gggccatcgc gttcgatttc cacgttggcc ggcgttagcc ccgggatgat gggcgtgccg
     4561 ccgtcacccg cgttcggatc gccgtagacg accaggttct tcaccttcgg gtcttcgagg
     4621 gcgttgccgc cggtcttctt gaccggcgtc gtcgccaggt agcgcgcgcc cgcgcgcgtc
     4681 gccttggcga gcgtcgagta gtggtagaag tagcgaccga actcggcggt ggccgcgaag
     4741 agcatcagca ggaccggcag cgctatcgcc agttcgatca attgcacccc gcgctcgctc
     4801 gccccgaaga ggcgcagccg ccgcacaatt ccgttcctca agttacgcat gtctctcgcc
     4861 tcactggtag agcacgggtt tagccaataa gccgttggca gcggcggtcc ccccgccgac
     4921 gccgccgcgc ccgacgatgg tcgggtcgtc gatgtactcg gcttcaagct cgccgccgct
     4981 ccccccgccg actttcttgc gcaggaagaa gaggccgaag cggttgaagc ggacaacgtt
     5041 gcggcccggg tcgtactcgc tctgcaagac gatggggatg atgacgatgc ggcggcc
//
LOCUS       DBNGOH010000044         4183 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4039454, whole genome shotgun sequence.
ACCESSION   DBNGOH010000044 DBNGOH010000000
VERSION     DBNGOH010000044.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4183)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4183)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4183
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4039454"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1135)
                     /locus_tag="AC159P_00915"
     CDS             complement(<1..1135)
                     /locus_tag="AC159P_00915"
                     /inference="COORDINATES: protein motif:HMM:NF024816.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3694039.1"
                     /translation="MPKAQPAGRAALKTVAEHKLLALAALCALACSGCGGWWAAPRTG
                     ASPPGPPRAVAALPDGGSDELVIRTLEEKVKADPEDFIAHNKLAGYYLRRQRETGEAS
                     YLALAARAAEASLAVLPAEHNPGGLALLAQSAYAAHDFAAARDHAERLAAIAPRESYP
                     YHVLGDALLELGDYEKAAAAFQRLGRLEQFRTAATETRLARLALLRGETEQAARRFEA
                     ALALAHEERVVERETVAWCRWQLGETAFSVGDYETAERHYRDALTNFPDYHRALASLA
                     RVRAARGDLASAVEHYERVTRLLPDPTYVAALGDLYRLTGREREAAQQYALVEKIARL
                     GESGGALHSRQLALFHADHGLKAEEAYQAAAREYASRRDIYGAD"
     gene            1829..3121
                     /locus_tag="AC159P_00920"
     CDS             1829..3121
                     /locus_tag="AC159P_00920"
                     /inference="COORDINATES: protein motif:HMM:NF025585.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4331 family protein"
                     /protein_id="HIR3694040.1"
                     /translation="MFRSHSKKTALAALALTLGLLTWLTPPARIDAADHGDAPYNSSD
                     QGIDGADAYAFLDPTDNTRVVLSLTLRGFIAAGENNNFGQFDPAVRHRFEIETTGDAR
                     ADRFIDITFARRTGATAPQVATITLMNGQTFTANSTPPSVCIAGTANCPPAATITDLG
                     GTGVRFFAGMRDDTFNFDIPAFAAATAALRACVANPSPPPTCVADALAIFNRGRDSFA
                     GYNIMNIALSIPKTLFGTPTNNVIGVQGVLQRRTPQLYPASPDVVRQTYPATGFGRWQ
                     TLDRNGIPAVNAVLIPFTRKDEFNAATPQDDANGRFAGDIVAALQALGTNATNINILA
                     GVAVTNGDMLRLNLNTPNTTLGTGETVATPNYAGFPNGRRPGDDVVDVLLFFIANQQP
                     VTDNANVNEVPIPNAFPFFPPPHQPRPAGAGAEDLTRN"
     gene            3176..>4183
                     /locus_tag="AC159P_00925"
     CDS             3176..>4183
                     /locus_tag="AC159P_00925"
                     /inference="COORDINATES: protein motif:HMM:NF027787.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5050 domain-containing protein"
                     /protein_id="HIR3694041.1"
                     /translation="MNSSTRGARRLCGTILSLAVLASGLALPGYSSSRAARDDSARAR
                     LKSRPGTQDGKAKGSRRAPASAKDGGQVQDHEEPPEGRIAFASDRDDNFEIYLVDPDG
                     GGLTRLTNNPAEEVGPAWSPDGTRLAFVSNRDGNQEIYTMNADGSGQTRLTNNPAADL
                     EPAWSPDGTKILFASNRDGNDEIYVMNADGSAQANLTNNGGDDNSPAWAPNGSAFAFS
                     TNRDGDKTEIYRANADGGGLVRLTNNSANDLAPTWAAGRITFQSDRDGNDEIYTMNEA
                     DGSNLIRLTNSVDDPATTGVNEALVFDLDPSRPTDGPRVDFVSNRDGASNLDIYLMNA
                     DG"
BASE COUNT          702 a         1507 c         1398 g          576 t
ORIGIN      
        1 cgtcggcgcc gtagatgtct cggcgcgagg cgtactcgcg cgccgcggcc tggtaagcct
       61 cttcggcctt caggccgtgg tcggcgtgga acagcgcgag ctggcggctg tggagcgcgc
      121 cgcccgactc gccgaggcgc gcgatcttct cgacgagcgc gtactgctgg gccgcctcgc
      181 gctcgcgccc cgtcaaccga tacaggtcgc cgagggcggc gacgtaggtc gggtcgggga
      241 ggaggcgcgt gacgcgctcg tagtgttcga cggcggacgc gaggtcgcca cgcgcggcgc
      301 ggacgcgggc caaagaggcg agcgcgcggt ggtagtcggg gaagttcgtg agcgcgtcgc
      361 ggtagtggcg ctcggcggtc tcgtagtcgc cgacggagaa ggccgtctcg ccgagctgcc
      421 agcggcacca cgcgaccgtc tcgcgctcga cgacgcgctc ctcgtgcgcg agcgcgagcg
      481 cggcctcgaa gcggcgcgcg gcctgctcgg tctcgccgcg cagcagcgcg agacgtgcga
      541 gccgcgtctc ggtcgccgcg gtcctgaact gttcgagccg ccccagacgc tggaaggcgg
      601 cggcggcctt ctcgtagtcg ccgagttcga gcagcgcgtc gccgaggacg tggtaggggt
      661 agctctcgcg cggggcgatg gcggcgaggc gctccgcgtg gtcgcgcgcg gcggcgaagt
      721 cgtgcgcggc gtaggccgac tgcgcgagca gggcgaggcc gccggggtta tgctccgcgg
      781 ggagcacggc gagcgacgcc tcggcggcgc gcgcggcgag ggcgaggtaa gaggcttcgc
      841 ccgtctcgcg ctggcgtcgg agataataac cggcgagctt gttgtgggcg ataaagtctt
      901 ccgggtcggc cttgaccttc tcttcgagcg tgcggatgac gagttcgtcc gagccgccgt
      961 ccggcagtgc ggcgaccgcg cgcggcgggc cgggcggcga cgcgcccgtt cgcggcgcag
     1021 cccaccagcc gccgcagccg ctacaggcca gcgcgcagag ggcggcgaga gcgagcagct
     1081 tgtgttcggc gacggtcttc aaggcggcac gtcctgcggg ttgggctttc ggcatcactc
     1141 ttccggcgac agaagtatct acgcgcgggt ggcccgcggc ggatgcggag aaagctcttc
     1201 acggcgattc tccgaaataa ggacggcggt cgtgcgggca ccgcggatgc gattctcgtc
     1261 cgggacgcgg gacatgctag ttgcacgcct cgcgcggtgt caacaggcga agtcggcggg
     1321 cgcacggcgt cactcgtgcg ccgcctttcg cggccccgca aacatttgcg ttgacaaggg
     1381 aatgctcctg ttataaaaac ggcttacgta gatcattcca ttgcaagctc tacctaagaa
     1441 gcggccctcg cgcggctacg tgtgtccgcg tcggccaccg acgccggcac tgcccgcctg
     1501 cacggcaagg ctcttttcac gcgcctctcg cgccctcgac gttcccagac actaaccgac
     1561 atttttatcg ttcacggggg gcgggcgaat ccggtgtcgc cggaggtttc gtatgggtga
     1621 tagtccgcca aaccggaaga cctattttac gcggcaaccc ccgtccgccg ccgccgaagt
     1681 ttgatcggct tgctatttca gtagtgttgt gtgcgccctc ccgccggacg gcggagtgag
     1741 gacctggcac atcgggcggt gcgacgccgt cgcccaccgc ccgcgccgaa gaaccgcttc
     1801 cgcggtacac caaccaggag aagaaaccat gtttcgctcg cacagtaaaa agaccgcgct
     1861 ggccgcgctg gcgttgacgt tggggctgct gacgtggctg acgccgccgg cgcgcatcga
     1921 cgccgccgac cacggcgacg cgccgtacaa cagctccgac cagggcattg acggggcgga
     1981 cgcctacgcc ttcctcgacc cgaccgacaa cacgcgggtc gtcctctcgc tgaccctgcg
     2041 cggcttcatc gccgccggcg agaacaacaa cttcgggcag ttcgacccgg cggtgcgcca
     2101 ccgcttcgag atcgagacca cgggcgacgc gcgcgccgac cgctttatcg acattacctt
     2161 cgcgcggcgc accggcgcga ccgcgccgca ggtggcgacg atcacgctga tgaacgggca
     2221 gaccttcacg gcgaattcga cgccgccctc ggtctgcatc gccggcacgg ccaactgccc
     2281 gcccgccgcg acgatcaccg acctcggcgg caccggcgtg cggttcttcg ccgggatgcg
     2341 cgacgacacg ttcaacttcg acatcccggc cttcgccgcg gcgaccgccg cgctgcgcgc
     2401 ctgcgtggcg aaccccagcc cgcccccgac ctgcgtcgcg gacgcgctcg ccatcttcaa
     2461 ccgcgggcgc gactcgttcg ccggctacaa catcatgaac atcgcgctga gcatcccgaa
     2521 gacgctcttc ggcaccccga cgaacaacgt catcggcgtg cagggcgtgc tccagcgccg
     2581 caccccgcag ctctacccgg cctcgcccga cgtggtgcgg cagacctacc cggctacggg
     2641 cttcggacgc tggcagacgc tcgaccgcaa cggcatcccg gcggtcaacg cggtgctcat
     2701 ccccttcacc cgcaaggacg agttcaacgc cgccacgccg caggacgacg ccaacgggcg
     2761 cttcgccggc gacatcgtcg ccgcgctcca ggcgctcggc accaacgcca cgaacatcaa
     2821 catcctggcg ggcgtcgccg tgacgaacgg cgacatgctg cgcctcaacc tgaacacgcc
     2881 gaacacgacc ctcggcacgg gcgagacggt ggcgacgccg aactacgccg gcttcccgaa
     2941 cgggcggcgg cccggcgacg acgtggtgga cgtgctcctc ttcttcatcg ccaaccagca
     3001 gcccgtcacc gacaacgcca acgtgaacga ggtgccgatc ccgaacgcct tcccgttctt
     3061 cccgccgccg caccaaccgc gccccgccgg cgccggcgcc gaagacctga cgcgcaactg
     3121 acgctcgtgc tgcacaaccc gagcgaggag taaacattcc atgaggagca aggtcatgaa
     3181 cagttcaacc cgcggcgcgc ggcgcctctg cggaacgata ctgtcgctgg ccgttctcgc
     3241 gagcggcctc gccttgcccg gttactcttc aagcagagca gcacgagacg acagcgcgcg
     3301 cgctcgcttg aaaagtaggc cgggcacaca ggacggcaaa gccaaaggct cccgccgcgc
     3361 acccgcgtcc gccaaagacg gcggccaggt gcaggatcac gaggagccgc ccgaaggccg
     3421 catcgccttc gccagcgacc gggatgacaa tttcgagatt tacctcgtcg atccggacgg
     3481 cggcgggctg acgcggctca ccaacaaccc cgccgaggag gtcggccccg cgtggtcgcc
     3541 cgacgggacg cgtctcgcct tcgtcagcaa ccgcgacggc aaccaggaaa tctacaccat
     3601 gaacgccgac ggctccggcc agacgcggct caccaacaac cccgccgccg acctcgaacc
     3661 cgcgtggtcg cccgacggga cgaagattct cttcgccagc aaccgcgacg gcaacgacga
     3721 gatttacgtg atgaacgcgg acggctcggc gcaggcgaac ctgaccaaca acggcgggga
     3781 cgacaacagc ccggcgtggg cgccgaacgg ctcggcgttc gccttctcga ccaaccgcga
     3841 cggcgacaag accgagattt accgcgccaa cgcagacggc ggcggcctcg tgcggctgac
     3901 gaacaactcg gccaacgacc tcgcgccgac gtgggccgcg ggccggatca ccttccagag
     3961 cgaccgcgac ggcaacgacg aaatctacac gatgaacgag gcggacggct cgaacctgat
     4021 ccgcctgacg aacagcgtgg acgacccggc gaccaccggc gtcaacgagg cgctcgtctt
     4081 cgacctcgac ccgtcgcgcc cgaccgacgg gccgcgcgtc gacttcgtca gcaaccgcga
     4141 cggcgcctct aacctcgaca tctacctgat gaacgccgac ggc
//
LOCUS       DBNGOH010000045         2663 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2573392, whole genome shotgun sequence.
ACCESSION   DBNGOH010000045 DBNGOH010000000
VERSION     DBNGOH010000045.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2663)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2663)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2663
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2573392"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..299)
                     /locus_tag="AC159P_00930"
     CDS             complement(<1..299)
                     /locus_tag="AC159P_00930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694042.1"
                     /translation="MSGKKSGCLSAARGRRKAYLVAALLASGCAGVALGCGWDGFPGS
                     VRFGDGATDAERSRLPPLPFRTGGAVEQRKTPARADDDVASWKQSAAEANKLWDAA"
     gene            complement(328..1983)
                     /locus_tag="AC159P_00935"
     CDS             complement(328..1983)
                     /locus_tag="AC159P_00935"
                     /inference="COORDINATES: protein motif:HMM:NF022784.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3142 domain-containing protein"
                     /protein_id="HIR3694043.1"
                     /translation="MRRLGHRRGAAVMMLVGLCLLVATSLASAAWRWASAPRAWAAGE
                     VPVAFWSWRTQAPAEEDVRRASEETGARALFLRAGQFDFADGRVMRVRAVEGRMPRGV
                     ELHLVYNGTRALLAEFGRVDEKVLAAAIAETFRNDRERAGGDGATIGGLQLDFDSPTR
                     LLARYARVLRAVREQLPPETRLSVTGLPTWMGAKELRDVLAVVDFWAPQFYGAEIPER
                     FEKAVPISSPRAVARDVARARALGKPFYAGLAAYGHAIVYSEGGELVALRGDLDPSRV
                     ARGSDFELVERRAFERRLKGEEAGGAARASEWRYVYRALAGTVVDGLTMRAGERVVFD
                     LPSGESLRASVREVREGAGEKLLGILLFRLPTRGDATTLNLRQIAAALSDAEANADVS
                     LRVEGGGGDGRVRGAAFARDPVRRDSAQRHSQDQLVVTATNAGAAGAGFGEGALVLTL
                     RVPRGGVRGVASLVGFDSFETLCAADGGRGVDEGGGVLSPCSPARAGFVRLKARGFES
                     GAVAVARRCVEGEWPSSLAVVLSMRVDDGRVWRRATALELSDR"
     gene            2377..>2663
                     /locus_tag="AC159P_00940"
     CDS             2377..>2663
                     /locus_tag="AC159P_00940"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3694044.1"
                     /translation="MSILIAEDNVAQRRYLRELLEREFASHAPVVEAADGEEATQLAL
                     KHRPELCVFDIQMPRLSGVKAAKTIWREFPTARIIFWTQFPHEIYINEIRK"
BASE COUNT          428 a         1067 c          811 g          357 t
ORIGIN      
        1 gcagcatccc agagcttgtt cgcctccgcc gcgctctgct tccacgaggc cacgtcatca
       61 tcggcgcgcg cgggcgtctt cctttgctcg accgcgccgc ccgtcctgaa cggcagcggc
      121 ggcaggcggc tgcgctcggc gtcggtcgcg ccgtcgccga agcgcacgga gccggggaaa
      181 ccgtcccacc cgcaacccag cgccacgccc gcgcatccgg acgccaacag cgcggcgacg
      241 agatacgcct tcctcctgcc gcgcgcggca gacagacacc cactcttctt tccgctcatc
      301 atctcggcct tcgcttcccg cgtttcatca ccggtcggac aactcaagcg ccgtcgcccg
      361 ccgccacacg cgcccgtcgt ccacgcgcat cgagaggacg acagcgagcg acgacggcca
      421 ctcgccctcg acacaccgac gcgcgacggc caccgcgccg ctctcgaagc cgcgcgcctt
      481 caaacgcacg aagcccgccc gcgcgggcga gcacggactg agcacgccgc cgccttcgtc
      541 aacgccccgc ccgccgtccg ccgcgcagag cgtctcgaac gagtcgaagc cgacgaggct
      601 tgccacgccg cgcacgccgc cgcgcgggac gcgcaaggtc aagacgagcg cgccctcgcc
      661 gaaccccgcc cccgccgcgc ccgcgttagt cgccgtgacc acgagctgat cttgcgaatg
      721 ccgctgcgct gaatcccgtc gaacagggtc gcgcgcgaac gccgcgccgc gcacacgacc
      781 gtcgccgccg ccgccctcca cgcgcaggct cacgtccgcg ttcgcttcgg cgtccgagag
      841 cgccgccgcg atctggcgga ggttgagcgt cgtcgcgtcg ccgcgcgtcg gcaggcggaa
      901 gaggaggatg ccgagcagct tttctccggc cccttcgcgc acttccctga cactcgcgcg
      961 gaggctctcg ccgctcggca agtcgaagac gacgcgctcg cccgcgcgca tcgtcaagcc
     1021 gtcaaccacc gtgcccgcga gcgcgcggta gacgtagcgc cactcgctcg cccgcgccgc
     1081 gccgcccgcc tcttcaccct tcaagcgccg ctcgaacgcg cggcgctcga ccagctcgaa
     1141 gtccgagccg cgcgccacac gcgaagggtc caggtcgccg cgcagcgcga ccagctcgcc
     1201 gccctccgaa tagacgatcg cgtggccgta agccgccagc cccgcgtaga agggcttgcc
     1261 caacgcgcgc gcgcgcgcga catcgcgcgc gaccgcgcgc ggcgaagaga tcggcaccgc
     1321 cttctcgaac cgctcgggaa tctccgcgcc gtagaactgc ggcgcccaga agtcaacgac
     1381 ggccagcacg tccctcaact ccttcgcgcc catccacgtc ggcaggcccg tcaccgagag
     1441 ccgcgtctcg ggcgggagct gctcgcgcac cgcgcgcaac acacgcgcgt agcgcgcgag
     1501 cagccgtgtc ggcgagtcga agtccaactg gaggccgccg atggtcgccc cgtccccgcc
     1561 ggcgcgctcc ctatcgttcc ggaaggtctc ggcgattgcg gcggccagaa ctttttcgtc
     1621 cacgcgcccg aactccgcga gcagcgcgcg cgtcccgttg taaaccagat gcagctcgac
     1681 gccgcgcggc atccgcccct cgaccgcgcg cacgcgcatc acacgaccgt cggcgaaatc
     1741 gaactgcccc gcccgcaaaa aaagcgcgcg cgcgcccgtc tcctccgacg cccgccgcac
     1801 gtcctcttcc gccggcgcct gcgtcctcca agaccagaac gcgaccggca cctcgcccgc
     1861 cgcccacgcg cgcggcgcgc tcgcccaacg ccacgccgcc gacgccagcg acgtggcgac
     1921 caacaggcac aggcccacca gcatcatcac cgccgcgccg cgtctgtgcc cgagccgtct
     1981 catcgttttc aaggagtccg ttctcgaaat atatgcgggg cgcgatcctc cccagtccac
     2041 gccccgcacc cgcgagcagg tcggcgccgc cgccacctgc gagagcatga cgggtggcgc
     2101 cgccgagcgt gattatcttc aaccccgcgg cgcgcggcga cgcgggcgcc aacagtcgtg
     2161 cgcgcgagca gatgcaccgc ccacgtgtgt ctctcccaac gcgccctcct gtggttaacc
     2221 acattgccaa agcggaggtc ggaggtcaga gttcagaggt cggaagtttt tgatagaatg
     2281 accggcgcgt tcggacggaa cggctctcgc gttcgaagcg acgcacggtg attacagtga
     2341 ctgacctctg acctctgact tctgacctct gcttttatgt ccatcctgat tgccgaagac
     2401 aacgtggcgc agcgccgtta cctgcgcgag ctgctcgaac gcgagttcgc ctcgcacgcg
     2461 cccgtcgtcg aggcggcgga cggcgaggag gcgacgcagc tcgccttgaa acaccggccc
     2521 gagctgtgcg tcttcgacat ccagatgccg cgtctgtcgg gcgtcaaggc ggcgaagacc
     2581 atctggcgcg agttccccac cgcgcgcatc atcttctgga cgcagttccc gcacgagatt
     2641 tacatcaacg agattcgcaa gat
//
LOCUS       DBNGOH010000046         2590 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_557349, whole genome shotgun sequence.
ACCESSION   DBNGOH010000046 DBNGOH010000000
VERSION     DBNGOH010000046.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2590)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2590)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2590
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_557349"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            275..1093
                     /locus_tag="AC159P_00945"
     CDS             275..1093
                     /locus_tag="AC159P_00945"
                     /inference="COORDINATES: protein motif:HMM:NF012451.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HIR3694045.1"
                     /translation="MVDYYKVLGVKPTAAAAEIKSAYRRLARQRHPDVNRGSDSAAKD
                     FALISLAYRTLSDPQERAYYDAQLKKRAAATSGEFTGSVFGSNNPHAQRLRRMAVQAR
                     FDRVVDRIIEAERRETFALQQAVFTTVTLFLSTFFVAMFKPRLWDRFDLVGRAIMLTL
                     FAIGLWHLAVRLKACFDHYTYQPKSIHDSIINASDEQSDKPFTRVAASTFLIVGYAAS
                     LAAGLFIGSHAQEVTIFNDLPYLFGQKFRIDLLFYPPIAVLIVDTMHTVASKID"
     gene            1560..1826
                     /locus_tag="AC159P_00950"
     CDS             1560..1826
                     /locus_tag="AC159P_00950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694046.1"
                     /translation="MAKRITERQRLALKLEHLNDTEVREVLDYIAIMESMTRATATPG
                     VWEDELVALLADARENKRARQAFEWESARRRAERRAAMNLPARV"
     gene            1823..>2590
                     /locus_tag="AC159P_00955"
     CDS             1823..>2590
                     /locus_tag="AC159P_00955"
                     /inference="COORDINATES: protein motif:HMM:NF025030.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HIR3694047.1"
                     /translation="MSRRKGSAQIRNSHRPARTHVAARAHANFFFAVGFVAVVFAGVF
                     DARAAAPLSPPAQDSHTPLQREIEKQKVRLSSADAEERRDAVTRLGAMRRPEASRAAA
                     AALGDAAAIVRATAARATLSLGSGEASTLLVPLLRDRDEFVRREAAYALGLTRHRFGV
                     SQLIVALETDKQASVRGAAAVALGQIGDPSAAPALAAALARRLPAPGFFARVTRRKVE
                     EDEFVRRAAAISLGQLASREAVPVLVAALSNERAPDDI"
BASE COUNT          441 a          962 c          779 g          408 t
ORIGIN      
        1 acaccacaaa aaagtcctcg cccccctcgg aaggccgccc gaggtcaaag caccgctccc
       61 ccggcacctc ctcctcacga acaaacgcat tcgtcctgct tcgagagatt gacgaaaact
      121 gtcctcaccc ccgttaattg gggtgggacg gatgcgtttg tttctggtat attgtcgccg
      181 tgagggcgac ctttcctgcc cgtgaatttt cgccagaatg ttccctgtca gggcattcca
      241 tcgccgactt tgagcggtct tcgggtaaag cgcggtggtc gattactata aagttttagg
      301 ggtcaagccg acggcggcgg ctgccgaaat caagtcggcg tacaggcgtc tggcgcgcca
      361 gcgacacccg gacgtgaacc gcggctcgga cagcgccgcc aaagacttcg ccctcatctc
      421 gctcgcctac cgcaccctca gcgacccgca ggagcgcgcc tattacgacg cccaactgaa
      481 gaagcgcgcg gcggcgacga gcggcgagtt caccggctcg gtcttcggct cgaacaaccc
      541 gcacgcgcag cgcctgcgcc ggatggccgt gcaggcgcgc ttcgaccgcg tcgtggatcg
      601 catcatcgag gccgagcgcc gcgagacgtt cgcgctccag caggccgtct tcaccaccgt
      661 cacgctcttc ctctcgacct tcttcgtcgc catgttcaag ccgcgcctct gggacaggtt
      721 cgacctcgtc gggcgcgcca tcatgctcac gctcttcgcc atcggcctct ggcacctcgc
      781 cgtccgcctc aaggcgtgct tcgaccacta cacctatcag ccgaagtcca tccacgactc
      841 gatcatcaac gcgagcgacg aacaatccga caagcccttc acgcgcgtcg ccgcctcaac
      901 cttcctcatc gtcggctacg cggcgagcct cgccgccggc ctcttcatcg gctcgcacgc
      961 gcaggaggtg acgatcttca acgatctgcc gtacctcttc ggccagaagt tccgcattga
     1021 cctcctcttc tacccgccca tcgccgtgct catcgtggac acgatgcaca ccgtcgcctc
     1081 caagatcgac tgaccggcag cggcccgctc gtcatccgac cgaccgccaa acgttcacca
     1141 cgccttcacg tcgtcgcccc gatgcgtctt gcgcgctgct tgactcgtcg tgtatattgc
     1201 gccgcaggcg gacgcttcgg agccgcttac aacgacagcc gcaacaacca atcgactacc
     1261 cgacaaggac ggcggcgccc gcgcgcggac ggcgcgcgcg cacgcacgcg cacggagtca
     1321 ggcgtttcgc gcttgccccc gcgacggcaa gcgagtgcgg gccgggttct ctgacggggc
     1381 ttgaaacagg agcctgttga ttctggagaa gcgaacgacg aagtcgaaga ggaagaaacg
     1441 cgcgctccac attgccgcgc cctgcaacgt agccgacggc ccgcctttcg tcagccgcac
     1501 acctcgttcg agctgaccgc ggccagccgc ccggcttcgc aaaggaagga gccggagaga
     1561 tggccaagcg gatcaccgaa cgtcaacgcc tcgcactcaa actcgaacac ctcaacgaca
     1621 cggaagtccg tgaagtgctc gactacatcg cgatcatgga gtccatgacg cgcgccacgg
     1681 ccacgcccgg cgtctgggag gacgagctgg tcgcgctcct cgccgacgcg cgcgagaaca
     1741 agcgcgcgcg acaggcgttc gagtgggagt cggcgcgccg gcgcgccgag cgccgcgccg
     1801 cgatgaacct cccggcgcgc gtatgagccg acgcaaaggc tccgcccaaa tccgtaattc
     1861 gcaccgaccg gcgcgcacgc acgtcgccgc gcgggcgcat gccaacttct tcttcgccgt
     1921 tggcttcgtc gccgtcgtct ttgccggcgt cttcgacgcc cgcgccgccg cgccgctttc
     1981 tccgcccgcg caagactctc acacgcctct ccaacgcgaa atcgagaaac agaaagtcag
     2041 gctctcttcc gccgacgcgg aggagcggcg cgacgcggtg acgcggctcg gcgcgatgcg
     2101 aaggccggag gcttcgcgcg ccgccgccgc cgcgctcggc gacgcggcgg cgatagtgcg
     2161 cgcgacggct gcgcgggcca cgctctcgct aggttcgggc gaggcctcga cgctcctcgt
     2221 cccgctgctc cgcgaccgcg acgagttcgt ccggcgcgag gccgcctacg cgctcggcct
     2281 gacgcgacac cgcttcggcg tgtcgcagct catcgtcgcg ctcgaaaccg ataagcaggc
     2341 ttccgtgcgc ggcgcggcgg cggtcgcgct cggccagata ggcgacccct ctgcggcgcc
     2401 cgccctcgca gcggcgctcg caagaaggct gcccgcgcca ggcttcttcg caagagtcac
     2461 acgccggaaa gttgaagaag acgagttcgt ccgccgcgcc gcggccatct cgctcgggca
     2521 gttggcgagc cgcgaagccg tgcccgtgct cgtcgccgcg ctctcgaacg agcgcgcgcc
     2581 cgacgacatc
//
LOCUS       DBNGOH010000047         8148 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3673214, whole genome shotgun sequence.
ACCESSION   DBNGOH010000047 DBNGOH010000000
VERSION     DBNGOH010000047.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8148)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8148)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8148
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3673214"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1491)
                     /locus_tag="AC159P_00960"
     CDS             complement(<1..1491)
                     /locus_tag="AC159P_00960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694048.1"
                     /translation="MTPLEVIQLIGYTTAAMLHMWIGAMLVRRRGDLGIIERVLTILA
                     FAIGVWHASNLFVALHSMLGLAEGTWETVLRVADTLAVVSVTLTYSLLLHVHLHLWAK
                     ARGRALNRTERVRVYLSYIPLAFLAYAVAKLWAGGYAPMFEKLSNLLLPFALWAAYVL
                     VLVAVTDLLIAYLSPSQSERRLMRTLGAAFVGIASLILLVYAAGFGQGSEAGNYLQTF
                     ANLGSLVPTALLAYHIYRYRYLELIFKESLIIASFAIVVLAVYLYGLRTLSAWATARF
                     GLRAAAIESILILALVLVASPLRRWLDNRFRELFEEEASLYREVAARISAHAGRYKQL
                     PELLGFVEERAAEGLGLRRVTLVAFDRDYQESDRDPESAEVFFEAAESAMWSEATKEE
                     WGAETEQRRAATTAGGILPGILQRSRELGWGPVEGEKYFGDSGYEIAYPLRREKRVLG
                     LLLVDAAPDALTYDVREVLNMLAGQVATAIEDCKLVEENVRLERRVA"
     gene            complement(1670..3271)
                     /locus_tag="AC159P_00965"
     CDS             complement(1670..3271)
                     /locus_tag="AC159P_00965"
                     /inference="COORDINATES: protein motif:HMM:NF012894.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HIR3694049.1"
                     /translation="MNKSSTPFILGSAARLALVLVACAVTAAGQNDHRRETVAVTYPL
                     EQSVTVKFRGTTRLPRLSGEAKVKRTGRRNTRVELEVKNLPRAFELGEVYTTFILWAV
                     SPEGRADNLGELKRSGSFIFNTKLDVTTPLQTFALLVTAEPHFLVRGPSRMVVLENLP
                     PRDPGDAQVATTSVQYIGNSSDYFKDPRVPDLADRDYIKTPVSLLGARQAVHLARFAG
                     ARRDAAEELNKAQAQLEVAENAWRLGQPEAEVDAEARRATSLAFAAEETAMANAAARK
                     GREEVARRDAAVREAEESAANANEQIAVLRDAFRKEQQAREIAEREAASSNQQLRELR
                     AEVARLREEVRDSRDDAESAKVKLARMEGERSAEQARVAAEQRAARQRDALLTLKSSL
                     ARFGSVKETERGVVLVLPDSVWAGARAYDLTPKAAATTVDPLAALLANNPDLQITIEA
                     FADSRGDEATLQRLTQDRAEALAAHLVSAGVDGARIRAAGMGVSNPVAANTTNAGRLK
                     NRRIEITLSPAASSDTSASNDGKQN"
     gene            complement(3501..3656)
                     /locus_tag="AC159P_00970"
     CDS             complement(3501..3656)
                     /locus_tag="AC159P_00970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5670 family protein"
                     /protein_id="HIR3694050.1"
                     /translation="MYVWLLAISLFVAWFVGKFILGKGGFIHIILLCAVSVAVVQFVH
                     DRRAARQ"
     gene            complement(3767..5296)
                     /locus_tag="AC159P_00975"
     CDS             complement(3767..5296)
                     /locus_tag="AC159P_00975"
                     /inference="COORDINATES: protein motif:HMM:NF024753.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HIR3694051.1"
                     /translation="MRTLSHDKLRALCLLILISRLLFTQTSTAQTTAARKADEAAAPQ
                     ATSSIHIRWQGKPGVERYRLQLATDARFNDIVFDQAVVGRQHVVRELTPGRYYWRVAP
                     AAKETGTYSAATEIVVVASNAVAPAQLGGWRTATGEVLRPVSAQLRSGGVVDLVGVNR
                     EGTTYAIDGANGGTLWAARYRPDAGAEQGGGDVSRFGPVLMQQRDVTSVVVAFDGGVR
                     ALAGDTGREAWRASLEGRPVSGAAGDLNADGKPEVVVVTRDPQWLYVLDGATGFVLTE
                     RKLGSEVVGQPFLLTEGRPRGVALASKDGAVGIFGAFGDPFRGAKMTSEVTTAPLVVK
                     RGGNALMVVGTREGLIALTLDELKPLGRIVPDADVVRGTLAAADVDGDGATEIVLLTE
                     RGRVALVSTVDGNVKWYAEGATGAGSAAFADVNTDGVLDVLVPGGDSFAVGFSGRDGS
                     LVWKVEEPGAKTRTGDVPARVLVVAPSPNGGAIVVGSDPSRVGLRAVELPKGAPKKAA
                     N"
     gene            complement(5296..5991)
                     /locus_tag="AC159P_00980"
     CDS             complement(5296..5991)
                     /locus_tag="AC159P_00980"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR3694052.1"
                     /translation="MIVDDDPNMRPLLAEYFRRLRYEVVDKETAEEALEPATSERFDC
                     FIFDVSMPGMSGLELLKRVRDRGVQTPAMFLTAHDALDDKVAGFQAGADDYLAKPFNP
                     RELEVRVEALLRRAGLPGQTDGEKPSKGERFKVGDLVIDKRRHEVTVRGERVDLTPLE
                     FHILERLASEPGRAWSRNDLLDEVWSTEYEGYQRNVDPHINRLRRKIESDPKNPRYVL
                     TVRGVGYKLNEAP"
     gene            6349..>8148
                     /locus_tag="AC159P_00985"
     CDS             6349..>8148
                     /locus_tag="AC159P_00985"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012869.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3694053.1"
                     /translation="MRDLKRLLVYLKPHWGKFTLATVAMLGVGLLQSAIGALIVPIFD
                     QALRQPDAAGERTPTLFGLQHFIPESGFAAWRTIAVLLIVFTILKGVAEYFSTYLMAR
                     VGQGAVYRLREDMYSHLHAQSADFFQRHRTNYLVSRLVGSAGAIETAVSHTLRDMLRE
                     SFTLVFFLSASFYYSWRLTLGSLLIAPVVGLLTARFGGSLRNLARESYEGGQQLVDTA
                     QEALSNHNIVKAYVAEERERERFSRVARQVMRANLRAAKINGAASPTIELIGVLAVAV
                     LLFFAQREIMGGHMTSAQFVTFLFFLFSSYDPMRKLSRLHNGMEQALAAVRHVWEVMD
                     EHEETPEKPDAVELAPLRDAIELKHVAFSYSADARRVLRDVSMRIPAGRMVALVGESG
                     GGKTTLTKLIPRFHDPSEGAVTWDGVSLRDATVASLRRNIALVTQETILFNDTVRYNI
                     SYSRPDSTDAEIEEAARVAFAHDFIKELPDGYDTVVGERGIFLSGGQRQRLAIARAVL
                     SDASVLVLDEATSALDAESERLVQKALANLTRGRTTVVIAHRLSTVRRADQIVVMERG
                     QIIETGKHAELLARDGHYRHLYELQFADEEETEQ"
BASE COUNT         1297 a         2776 c         2725 g         1350 t
ORIGIN      
        1 cgccacgcgc cgctcgagcc tcacgttctc ttccacaagc ttgcagtcct cgatggccgt
       61 cgccacctgc cccgcgagca tgttgagcac ttcgcgcacg tcgtaggtca gcgcgtcggg
      121 cgcggcgtca acgagcaaca gccccagcac gcgtttttcg cgccgcagcg ggtaggctat
      181 ctcgtacccg ctgtcgccga aatacttttc gccctcgacc ggcccccagc caagctcgcg
      241 cgagcgctgt aaaatgcccg gcagtatccc ccccgctgtt gttgccgcgc gcctctgttc
      301 ggtctccgcg ccccattcct ctttcgtcgc ctcgctccac atcgcgcttt cagcagcctc
      361 gaagaagacc tccgccgact cggggtctct atcggactcc tggtagtcgc ggtcgaaggc
      421 gacgagcgtc acgcgccgca ggccgagtcc ttcggcggcg cgctcttcga cgaagccgag
      481 tagctccggc agttgcttgt agcgccctgc gtgcgcgctg atgcgcgcgg ccacttcgcg
      541 gtagagcgaa gcctcctctt cgaacagctc gcggaagcgg ttgtcgagcc agcggcgcaa
      601 gggggaggcg acgagcacga gcgctagaat cagtatggac tcgatggcgg cggcgcgcag
      661 gccgaagcgt gcggtggccc acgccgagag cgtccgcagc ccgtagaggt agacggcgag
      721 cacgacgatg gcgaacgagg cgatgatgag gctctctttg aagatcagtt cgagatagcg
      781 gtagcggtag atgtgatacg cgagcagcgc cgtcggcacg agcgagccga ggttggcgaa
      841 cgtctggagg tagttccccg cttccgaccc ctgcccgaag cccgccgcgt agacgagcag
      901 gatcagcgag gcgatgccga cgaaggccgc gccgagcgtc cgcatcagcc gccgctcgct
      961 ctgcgacggc gagaggtacg cgatgagcag gtctgtgacg gcgacgagca cgaggacgta
     1021 ggcggcccac agcgcgaacg gcaacagcag gttcgagagc ttctcgaaca tcggcgcgta
     1081 gccgcccgcc cacagcttcg cgaccgcgta ggcgaggaac gcgagcggga tgtaggagag
     1141 gtagacgcgg acgcgctccg tgcggttgag cgcgcgcccg cgcgccttcg cccacagatg
     1201 caggtggacg tggagcagca gcgagtaggt cagcgtcacg ctgacgacgg ccagcgtgtc
     1261 cgcgacgcgg agcacggtct cccaagtccc ttcggcgagg ccgagcatcg agtgcagcgc
     1321 gacgaagagg ttcgacgcgt gccagacgcc gatggcgaag gcgaggatcg tcagcacgcg
     1381 ctcgatgatg ccgaggtcgc cgcgcctgcg gacgagcatc gcgccgatcc acatatgcag
     1441 catcgccgcc gtggtgtagc cgatgagctg tatgacttcg agcggtgtca tgtcgaaacg
     1501 agaagcggca cgttaacagg acattctaag ggggaaaagg gggaaagagg aagaagggga
     1561 aaagccaaag tttaaaaggc gaaaagggga aaaggcgaaa aggaaggaaa gcaaactgct
     1621 tcttcccctt atcgcctctt ccccttttga cctttccccc ctcggttttt cagttctgct
     1681 tgccgtcgtt cgacgccgat gtatcactgg aggcggcggg cgagagtgtg atctcgatgc
     1741 ggcggttctt gagccgaccg gcgttggtcg tgttggcggc gacggggttc gagacgccca
     1801 tgcccgcggc gcggatgcgt gcgccgtcaa cgcccgcgga gacgaggtgt gcggcgagcg
     1861 cttcggcgcg gtcttgcgtg agccgctgca aggtggcctc gtcgccgcgg ctgtcggcga
     1921 aggcttcgat ggtgatttgc aggtcggggt tgttggccag gagcgccgcg agcggatcga
     1981 cggtcgtggc cgcggccttc ggcgtcaggt cgtaggcgcg cgccccggcc cacaccgagt
     2041 cgggcaggac gaggacgacg ccgcgctccg tctccttgac gctgccgaaa cgcgcgaggc
     2101 tcgacttcaa agtgagcagc gcgtcgcgct gccgggccgc gcgctgctcg gcggcgacgc
     2161 gcgcctgctc ggcgctgcgc tcgccctcca tgcgcgcgag cttgaccttc gcgctctcgg
     2221 cgtcgtcgcg cgagtcgcgc acctcttcgc gcagccgcgc gacttcggcg cgcagctcgc
     2281 ggagctgctg gttggagctg gccgcttcgc gctcggcgat ctcgcgcgcc tgctgttcct
     2341 tgcggaacgc gtcgcgcagc acggcgatct gctcgttggc gttggctgcc gattcctcgg
     2401 cctcgcgcac ggcggcgtcg cggcgcgcga cctcctcgcg ccccttgcgc gcggcggcgt
     2461 tggccatcgc cgtctcctcc gccgcgaagg cgaggctcgt ggccctgcgc gcctctgcgt
     2521 ccacctccgc ttcgggctgg ccgaggcgcc acgcgttctc cgcgacttcg agctgcgcct
     2581 gcgccttgtt cagctcttcg gcggcgtcgc ggcgcgcgcc cgcgaagcgc gcgagatgga
     2641 cggcctgccg cgcgccgagc agcgagacgg gcgtcttgat gtagtcgcgg tcggcgaggt
     2701 cgggcacacg cgggtctttg aagtagtcgc tggagttgcc gatgtactgg acgctggtgg
     2761 tggcgacctg cgcgtcgccg gggtcgcgcg gcgggaggtt ttcgaggacg accatgcggc
     2821 tggggccgcg caccagaaag tgcggctcgg cagtgacgag gagcgcgaag gtctgcaagg
     2881 gcgtcgtcac gtcgagcttg gtgttgaaga tgaagctgcc gctgcgtttg agctcgccga
     2941 ggttgtcggc gcggccctcg ggcgagacgg cccagaggat gaaggtcgtg tagacctcgc
     3001 ccagctcgaa ggcgcgcggc aggttcttga cctcaagctc gacgcgcgtg ttgcgccgtc
     3061 ccgtccgctt gaccttcgcc tcgcccgaga gacgcggcag ccgcgtcgtc ccgcggaact
     3121 tgaccgtcac ggactgttcc agaggatacg tgacggcgac cgtctcgcgg cggtggtcgt
     3181 tctgcccggc agccgtgacg gcgcacgcga cgagcacgag cgcgagccgc gccgccgaac
     3241 cgaggatgaa aggagtgctg cttttattca ttcgaattcg ctccttgctg cgcttcgcgc
     3301 cggtcgtggc gcctgcgggg acagaccgcg ccgtgtgcgc gaaaatacct ctgacgagta
     3361 attgatgctc cgaacgcccc gcgctgttgc cggtcgaggc ttcccgcgcc gccccattgt
     3421 ttcaggccgg gccgacccgt gacaagccgg acgtttcgcg cacgagattt ttaaggggag
     3481 agacggcggc gggcacggct tcactgccgc gcggcgcggc ggtcgtggac gaactgcacg
     3541 acggccacgg agaccgcgca gagcagtatg atgtggatga agccgccctt gccgagaatg
     3601 aacttcccga cgaaccatgc gacgaagagc gagatggcga gcagccagac gtacataaag
     3661 taaagtgggg gagaaaggga gaaagggagt ggggagacag gaatcgacgc tacgtaaaca
     3721 cgacttctta tctccccatc tccccttctc cctctctccc cttccgtcag ttcgccgcct
     3781 tcttcggagc gcctttcggc agttcgacgg cgcggaggcc gacgcgcgaa gggtcgctgc
     3841 cgacgacgat ggcgccgccg ttcggcgaag gcgcgacgac gagcacgcgc gcgggtacgt
     3901 cgcccgttcg ggttttggcg cccggctctt cgaccttcca gacgagcgag ccgtcacggc
     3961 ccgaaaagcc gacggcgaat gaatcgccgc cgggcacgag cacgtcgagc acgccgtctg
     4021 tgttcacgtc ggcgaaggcg gcggacccgg cgccggtcgc gccctctgcg taccacttca
     4081 cgttgccgtc aaccgtgctc acgagcgcca cgcgcccgcg ctccgtgagc agcacgatct
     4141 cggtcgcgcc gtcgccgtcc acgtccgcgg cagccagcgt cccgcgcacc acgtccgcgt
     4201 ccggcacgat gcgcccgagc ggcttcaact cgtcgagcgt cagcgcgatc agcccctcgc
     4261 gcgtgccgac gaccatcaaa gcgttcccgc cccgcttcac gacgagcggc gcggtcgtca
     4321 cctccgaagt catcttcgcg ccgcggaacg ggtcgccgaa ggcgccgaag attccgaccg
     4381 cgccgtcctt cgaagcgagc gccacgccgc gcggccggcc ctccgtcagc aggaacggtt
     4441 ggccgacgac ctctgagccg agtttcctct ccgtgagcac gaagcccgtc gcgccgtcga
     4501 gcacgtaaag ccattgcggg tctctcgtca cgacgacgac ctccggcttg ccgtccgcgt
     4561 tcaggtcgcc cgcggccccg ctcaccggtc ggccttcgag gctcgcgcgc caagcctccc
     4621 tgcccgtgtc gcccgccagc gcgcgcacac ccccgtcgaa cgcgacgaca acactcgtca
     4681 cgtccctctg ttgcatcagg acggggccga agcgagaaac gtcgccgccg ccctgctcgg
     4741 cgcccgcgtc gggccgatag cgcgcggccc agagcgtgcc accgttcgcg ccgtcgatgg
     4801 cgtaggtcgt cccctcacgg ttcacgccga cgagatcgac gacgcccccc gaacgcagtt
     4861 gcgcagagac ggggcgcaga acttcgccgg tcgccgtgcg ccagccgcca agctgcgcgg
     4921 gcgcgacggc gttcgacgcg acgacgacga tctccgtcgc cgccgagtag gtgcccgtct
     4981 ctttcgcggc gggcgcgacg cgccagtagt agcggcccgg cgtcagctcc ctgacgacgt
     5041 gctgccgccc gacgacggcc tggtcgaaga cgatgtcgtt gaagcgcgcg tccgtggcga
     5101 gttgcaggcg gtagcgctcg acgccgggct tgccctgcca gcggatgtgg atggaggaag
     5161 tggcctgcgg ggcggcggct tcgtccgcct tccgcgccgc cgttgtttgt gccgtggaag
     5221 tctgcgtgaa gaggagtcgg gagatgagga tgaggaggca gagggcacgg agtttatcgt
     5281 gcgagagtgt gcgcatcatg gggcctcgtt cagtttgtag ccgacgccgc gcaccgtcag
     5341 aacgtagcgt ggatttttgg ggtcggactc gatcttgcgg cgcaggcgat tgatgtgcgg
     5401 atcgacgttg cgctggtagc cttcgtactc cgtgctccac acttcgtcca aaaggtcgtt
     5461 gcgcgaccac gcgcggcccg gctcgctcgc cagccgttcg aggatgtgaa actcaagcgg
     5521 cgtgagatcg acgcgctcgc cccgcaccgt cacctcgtgg cggcgcttgt cgatgacgag
     5581 gtcgccgacc ttgaagcgct cgcccttcga cggcttctcg ccgtcggttt gaccgggcaa
     5641 gcccgcgcgc cggagcagcg cctcgacccg cacctcaagc tcgcgcgggt tgaagggctt
     5701 ggcgaggtag tcgtccgccc cggcctgaaa gcccgccacc ttgtcgtcga gcgcgtcgtg
     5761 cgccgtgagg aacatggcgg gcgtctggac gccccggtcg cgcacacgct tgagcagctc
     5821 aagccctgac atgcccggca tcgacacgtc gaagatgaag caatcgaagc gctcggaggt
     5881 ggccggttcg agagcttcct cggcggtctc tttatccacg acctcgtacc tgagccgccg
     5941 aaaatactct gccagcagcg ggcgcatgtt cgggtcgtcg tcaactatca aaagtcgaat
     6001 gctattttct gctacggcca ccttagttta gactcggaac cttcatgttt gggatcatgc
     6061 agagcgcgca actcgattat aaccgaatca caaccaattg cacccgcgcg ggccgcgtcc
     6121 gtcccgtttg gcgtgtttcg cggcggcgag ttcgggccgg gggcgtccgt tttcgaagtc
     6181 acccggcggc ggcccgccgc actaaatccg agacccgaat taatgcgctc gaccccgcga
     6241 agcacggcgc ccggtcttga cgcccaaagc tcttgctacg tatcttagcc ggggcgaatc
     6301 tttttccctt cccaatagcc ggcgtccggg gcacgaagcc gaaagtcaat gcgcgaccta
     6361 aagcgactac tggtttacct caaaccgcac tgggggaagt tcacgctggc gaccgtcgcg
     6421 atgctcggcg tcggcctcct tcagagcgcc atcggcgcgc tcatcgtccc gatcttcgac
     6481 caggctttga ggcagcccga cgccgcgggt gagcgtactc cgacgctctt cggattgcaa
     6541 cacttcattc ccgagtcggg cttcgccgcg tggcgcacca tcgcggtgct gctcatcgtc
     6601 ttcaccatcc tcaagggcgt cgccgaatac ttctcgacct acctgatggc gcgcgtcggg
     6661 cagggcgcgg tctaccgcct gcgcgaagac atgtattcgc acctgcacgc gcagagcgcc
     6721 gacttcttcc agcggcaccg gacgaactat ctcgtctcgc ggctcgtcgg ctcggcgggc
     6781 gccatcgaaa cggcggtctc gcacacactg cgcgacatgc tccgcgagag cttcacgctc
     6841 gtcttcttcc tctcggcctc gttctactac tcgtggcggc tcacgctcgg ctcgctcctc
     6901 atcgcgccgg tggtcgggtt gctgaccgcg cgcttcggcg gcagtctcag gaacctcgcg
     6961 cgcgagtcct acgagggtgg ccagcaactc gttgacacgg cgcaggaggc gctctcgaat
     7021 cacaacatcg tcaaggcgta cgtcgccgaa gagagagagc gcgagcgctt ctcgcgcgtc
     7081 gcgcggcagg tgatgcgcgc gaacctgcgc gccgcgaaga tcaacggcgc cgcctcgccc
     7141 accatcgagc tgatcggcgt gctcgcggtc gctgtcctcc tcttcttcgc gcagcgcgag
     7201 atcatgggcg ggcacatgac ctccgcgcag ttcgtcacct tcctcttctt cctcttctcc
     7261 tcttacgacc cgatgcgcaa gctctcgcgc ctgcacaacg ggatggagca ggcgctcgcc
     7321 gccgtgcgcc acgtctggga ggtgatggac gaacacgagg agacgcccga gaagcccgat
     7381 gccgtcgaac tcgccccact gcgtgacgcc atcgaactca agcacgtcgc cttcagctac
     7441 tcggcggacg cgcgccgcgt cctgcgcgac gtgtcgatgc gcatccctgc ggggcgcatg
     7501 gtcgcgctcg tcggcgagtc gggcggcggc aagacgacgc tcacgaaact gatcccgcgc
     7561 ttccacgacc cttcggaagg cgcggtcacg tgggacggcg tcagcctgcg cgacgcgacg
     7621 gtcgccagct tgcgccgcaa catcgcgctc gtcacgcagg agaccatcct cttcaacgac
     7681 accgtccgct acaacatctc ctacagccgc cccgactcga cggacgccga gatcgaagag
     7741 gccgcgcgtg tcgccttcgc gcacgacttc atcaaggagc tgcccgacgg ctacgacacg
     7801 gtggtcggcg agcgcgggat tttcctgtcg ggcggccagc ggcaacggct ggcgattgcg
     7861 cgcgcggtgc tctccgacgc ctccgtgctc gtcctcgatg aggcgacctc cgcgctcgac
     7921 gcagaaagcg agcggctggt gcagaaggcg ctcgccaacc tgacgcgcgg gcgcacgacc
     7981 gtcgtcatcg cgcaccgcct ctcgaccgtg cgccgcgccg accagatcgt cgtcatggag
     8041 cgcggccaga tcatcgagac cggcaagcac gcggagttgc tcgcgcgcga cggccactac
     8101 cggcacctct acgagctgca attcgccgac gaggaagaga cggaacag
//
LOCUS       DBNGOH010000048         2277 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1107357, whole genome shotgun sequence.
ACCESSION   DBNGOH010000048 DBNGOH010000000
VERSION     DBNGOH010000048.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2277)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2277)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2277
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1107357"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1006)
                     /locus_tag="AC159P_00990"
     CDS             complement(<1..1006)
                     /locus_tag="AC159P_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012528447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HIR3694054.1"
                     /translation="METLDRNYVNIEDEMRRSYLDYAMSVIIGRALPDVRDGFKPVHR
                     RVLWAMHELGNQYNKAYKKSARIVGDVIGKYHPHGDSAVYETIVRMAQDFSMRYPLVD
                     GQGNFGSIDGDTAAAMRYTEARMARLTSEILADIEKETVNFQPNYDESLSEPAVMPTR
                     VPSLLLNGSEGIAVGMATKIPPHNLTEIIDATIALIKKPSTKAEELLKLVPGPDFPTG
                     GFIYGREGIKQAYLTGRGIIQMRARAGIDRIGRGTTERDAIIITEIPFQVNKARLIER
                     IADLVREKQIDGISSDGLRDESDKDGMRIVIELKRGEVADILLNKLFKHTPMESVFGI
                     N"
     gene            1361..2134
                     /locus_tag="AC159P_00995"
     CDS             1361..2134
                     /locus_tag="AC159P_00995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694055.1"
                     /translation="MEEGGKSLRVRERLKLTLPVRVHCRESAAHEWVELSRLVDVTPF
                     GARFTLAHPTERGRLMHLTMPLPRQLRCFDHIEDQYRVWALVRHAAPLVAAPEAGPST
                     GAGLLRFDVGVAFTGKHPPASYQRDPTTLYEVDRLAAEGNLWAVRERPSQAVGGPQLQ
                     RSQKTRIQMAVVVLVELFDERGAITASEQTVTENISRRGASVMTSLDAERGRFVRVTS
                     AEHGLSVVAAVRARRTGPDGIPRLHLEFVDRQWPLEGIE"
BASE COUNT          377 a          719 c          763 g          418 t
ORIGIN      
        1 tgttgatgcc gaacaccgat tccatcggcg tgtgcttgaa cagcttgttc agcaggatgt
       61 ccgcgacttc accacgcttg agctcgatga cgatgcgcat gccgtccttg tcggactcgt
      121 cacgcaaccc atcgctggaa atgccatcga tctgcttctc gcggaccagg tccgcgatgc
      181 gctcgatgag gcgcgccttg ttcacctgga agggaatctc tgtgatgatg atggcgtcgc
      241 gctcggtcgt gccgcgcccg atgcggtcga taccggcgcg ggcgcgcatc tggatgatgc
      301 cgcggcccgt gaggtacgcc tgcttgatgc cctcgcgccc gtagatgaag ccgcccgtcg
      361 ggaaatccgg gccggggacg agcttgagca attcttcggc cttcgtgctc ggcttcttga
      421 tgagggcaat ggtggcgtcg atgatctccg tcagattgtg cggcgggatt ttggtcgcca
      481 tgccgacggc gatgccttcc gagccgttca agaggaggct cggcacgcgc gtcggcatca
      541 ccgccggctc gctcaaactc tcgtcgtagt tcggctggaa gttgacggtc tccttctcga
      601 tgtcggcgag aatctcggag gtgagccgcg ccatgcgcgc ctcggtgtag cgcatcgcgg
      661 cggcggtgtc gccgtcgatg gagccgaagt tgccctgccc gtccacgagc gggtagcgca
      721 tggagaagtc ctgcgccatg cgaactatcg tttcgtagac ggcgctgtcg ccgtgcgggt
      781 ggtacttgcc gatcacgtcg ccgacgatgc gcgccgactt cttgtaagcc ttgttgtact
      841 gattgcccaa ctcgtgcatc gcccacagca cgcggcggtg gacgggcttg aagccgtcgc
      901 gcacgtcggg cagggcgcgc ccgataatga ccgacatcgc gtaatcgaga tacgagcggc
      961 gcatctcgtc ttcgatgttg acgtagttgc gatccaaagt ttccatcgaa taaacctttc
     1021 cggtacaatc tgccgcgccc gcgcttttcg agttcgggcg cgcctcaaag tcactgacaa
     1081 gcaaaagaga actggcggcg gacggggctt ccgaggggcc tgaagggcgg cccgcccgcg
     1141 gcccgattcg actggcgcga agggactttc ccgtgcggga aacggctggc ccgaagcacg
     1201 acatattgta gcccggaaag cggcttttgg caaccgcccc gatgggccga aagtcctgca
     1261 aaatcagtgt ttttggcccg gaaagaggcg tcttttcgga cacttgggcg ggcgcaatat
     1321 gttgggcttt caagcagggt gcaaggcggg cgtttcgagg atggaagagg gaggaaaatc
     1381 actgcgcgtg cgcgagcgtt tgaagctcac gctgcccgtc cgcgtccact gccgcgagtc
     1441 ggcggcgcac gagtgggtgg agctgtcgcg cctcgtggac gtgacgccgt tcggcgcgcg
     1501 cttcacgctc gcgcacccga ccgagcgcgg gcggctgatg catctcacga tgccgctgcc
     1561 gcgccagctc cgctgcttcg accacatcga agaccagtac cgcgtctggg cgctcgtgcg
     1621 ccacgccgcg ccgctggtcg ccgcgcccga ggcggggccg tcaaccgggg cgggcctcct
     1681 ccgcttcgac gtgggcgtcg ccttcacggg caagcacccg cccgcgagct accagagaga
     1741 cccgacgacg ctctacgaag tagaccggct cgcggcggaa ggcaacctgt gggcggtgcg
     1801 cgagaggccg agtcaggccg tgggcgggcc gcagttgcag cgctcgcaga agacgcggat
     1861 tcagatggcg gtcgtcgtcc tcgtcgagct gttcgacgag cgcggcgcga tcacggcgag
     1921 cgagcagacc gtgaccgaga acatcagccg ccgcggcgcg tccgtgatga cctcgctcga
     1981 cgccgagcgc gggcgcttcg tccgcgtcac cagtgccgag cacggcctct cggtcgtcgc
     2041 cgccgtgcgc gcgcgccgca caggccccga cggcatcccg cgcctccacc tcgaattcgt
     2101 tgaccggcag tggccgctgg aaggaataga gtaaaagccg tgaatcgtga atcggggttg
     2161 agtgtcgctc acgctgaatg cgacctctca gccccgcggc cgctcaaacg attcacgatt
     2221 gacgattcac ggttgacgag tccttatgaa acgcatcgtc ttcccgcgtg aagtgtt
//
LOCUS       DBNGOH010000049         2709 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3123544, whole genome shotgun sequence.
ACCESSION   DBNGOH010000049 DBNGOH010000000
VERSION     DBNGOH010000049.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2709)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2709)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2709
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3123544"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            249..527
                     /locus_tag="AC159P_01000"
     CDS             249..527
                     /locus_tag="AC159P_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013628441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HIR3694056.1"
                     /translation="MISLVDLLILLLIAGVCGALGQAIGGFTRGGCLVSIALGFIGAL
                     LGSFLARKLGLPELFAVNIGGQNFPIIWSIIGSALFVAVIGLISRSRA"
     gene            729..2453
                     /locus_tag="AC159P_01005"
     CDS             729..2453
                     /locus_tag="AC159P_01005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694057.1"
                     /translation="MSATATARYTERTRASAPDLGYFRQHYPATVASFGTLPRREEWL
                     RRVWETEERWRSALGANGHAEEVIVRGAPPAGREAEGRFDVIYAGGTLGLLHASALAR
                     RHERRVLVFDEGEVGRAGRGWNVSDEELKEFERAGLLSAEEIESAVVNRYDAGFVKFH
                     DAASRVKADPLWVSGVMDVAVDAERLFELAAGKLRRRKGCAIEGGLRFVRAYVEPHRV
                     TVETEDVRRGGRRRLFAARLFVDAGGGDSPVARQVRGGRPVTHVCPTVGTVARGYARG
                     EGPDKVDFNAGELLVSTEDASDHRQLIWEGFPGHPARGEYAACLFFHDAADSPADKSL
                     LSLFERYFETLPAYKRPGAQWRVERPVFGYAPSTRRQKWRGRERRADERVMLVGEALG
                     QSNAWAFGGEGGHLRALHRQTHLLHLALEADMLDADSLAAVSDFGPRGARMNALAEFM
                     RPTPKGAPSAVNETLNAVMAALHGLDERVRRQLLQDRMTPGALKSLLARTVKLYPRIL
                     ARVREHLGARGTLCWIADLADEFWSERRGRTAAAGGAGGAEDRAADAAGEFARRVALY
                     KNGRGAEG"
BASE COUNT          369 a          910 c          974 g          456 t
ORIGIN      
        1 ggggctttcg ccgcgggttg cctttatcgg tgaggcggcg ccttgcgggt cgcgccgctt
       61 tcgaacctgc gggccgtcgg tggcccgccg caaagattat agagaggccg cgcggatttg
      121 ggaagggaaa gattttcctg cgccgctttt cactcgaacg cgggccgaaa atttttgcca
      181 gcgcgggcgc gcgggcgcta tgctgtgggg acactgtcag gcgcgacaca gttcgaggga
      241 ggtgggggat gatatcactc gttgacttgc tgattctgtt gctcatcgcg ggcgtgtgcg
      301 gcgcgctcgg gcaggccatc ggcggcttca cgcgcggcgg ctgtctggtc tccatcgcgc
      361 tcggctttat cggcgcgctg ctcggctctt tccttgcgcg caagctcggc ctgcccgagc
      421 tgttcgccgt caacatcggc gggcagaact tccccatcat ctggtcgatc atcggctccg
      481 cgctcttcgt cgccgtcatc ggcctcatct cgcgcagccg agcgtgaaga ggtcggaggt
      541 cggaggtcgg aggtcagtga aagataaact gcttgtgttt taactgacct ccgacctccg
      601 acctcttctt ttcattccct gccgcatcgg ttacactcgc gcacacgcac aagctccctt
      661 ttggtaattc gtttttcgcg cgcgcgttcg tcccctgacg cgccgcggtt cgctttggta
      721 gacgatttat gagcgccacg gccacggcaa ggtacacgga gcgcacgcgc gcgagcgcgc
      781 ccgacctcgg ctacttcaga caacattatc cggcgacggt ggcgagcttc ggaacgctgc
      841 cgcgccgcga ggagtggctg cggcgcgtgt gggagacgga ggagcgctgg cgctcggcgc
      901 tcggcgcgaa cggtcacgcc gaggaggtga tcgtgcgcgg cgcgccgccc gcgggccgcg
      961 aggccgaagg ccggttcgac gtgatctacg cgggcggcac gctcggcctg ctgcacgcgt
     1021 cggcgctggc tcgccgtcac gagcggcgcg tgctggtctt cgacgagggc gaggtcgggc
     1081 gcgcgggccg cggctggaac gtctcggacg aggagctgaa ggagttcgag cgcgcgggcc
     1141 tgctctccgc cgaagagatc gagagcgcgg tcgtcaaccg ctacgacgcg ggcttcgtga
     1201 agttccacga cgcggcgtcg cgcgtgaagg cagacccgct gtgggtgagc ggcgtgatgg
     1261 acgtggcggt cgatgccgag cggctgttcg aactcgccgc cggtaaactc cgacggcgca
     1321 aggggtgcgc catcgagggc gggctgcgct tcgtgcgcgc ctacgtcgag ccgcaccgcg
     1381 tcacggtcga gacagaggac gtgcggcgcg gcgggcgccg gaggctgttc gcggcccgcc
     1441 tgttcgtgga cgcagggggc ggcgactcgc ccgtcgcgcg gcaggtgcgc ggggggcgcc
     1501 cggtgacgca cgtgtgcccg acggtcggca ccgtcgctcg cggctacgcg cggggcgaag
     1561 ggccggacaa ggttgacttc aacgccggtg agcttctggt cagcaccgaa gacgcctccg
     1621 accaccggca gctcatctgg gagggcttcc ccggtcatcc cgcgcgcggc gagtacgcgg
     1681 cctgcctctt cttccacgac gcggcggact cgcccgcaga caagtctttg ctctcgctct
     1741 tcgagcgcta cttcgagacg ctgcccgcct acaagcggcc cggcgcgcag tggcgcgtcg
     1801 agcgccccgt cttcggctac gcgccttcga cgcggcggca gaagtggcgc gggcgcgagc
     1861 gacgtgcgga cgagcgcgtg atgctcgtcg gcgaagcgtt gggccaatcg aacgcgtggg
     1921 ccttcggcgg cgagggcggg cacctgcgcg ccctgcacag gcagacgcac ctgctgcacc
     1981 tcgcgctcga agcggacatg ctcgacgccg actcgctcgc cgcggtttcg gacttcgggc
     2041 cgcgcggcgc gcgcatgaac gcgctggccg agttcatgcg cccgacgccg aagggcgcgc
     2101 cctccgccgt caacgagacg ctgaacgcgg tgatggccgc gctgcacggg ctggacgagc
     2161 gtgtccgccg ccaactgctg caagaccgca tgacgcccgg cgccctgaag agcctgctcg
     2221 cgcgcaccgt caagctctac ccgcgcatcc tcgcgcgcgt gcgcgaacac ctcggcgcgc
     2281 gcggcacgct ctgctggatc gcggacctcg cggacgagtt ctggagcgag cggcgcgggc
     2341 gcaccgccgc cgcgggcggc gcgggcggcg ccgaagaccg cgccgcggac gcggcgggcg
     2401 agttcgcgcg ccgcgtcgcc ctctataaaa acgggcgggg cgcggaaggc tgagagacct
     2461 tacacgcccc gctggctcgc acgtcttcga ttgttgtcgt gaggtgatga ccggctccgc
     2521 gcccggcgga gccggttacg cgccgtcgtc gcagacgatc tcgaccgtgt cacccggctc
     2581 caattctcgt actgtctcgc cgttgacctt gatccgtaga agcctgccgt cggcgttctt
     2641 gtacttcttc cgcccgccgg cgtggccgtt atccttgaac ttgtccgaca cgtttagagt
     2701 gacggatcc
//
LOCUS       DBNGOH010000050         6044 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3490292, whole genome shotgun sequence.
ACCESSION   DBNGOH010000050 DBNGOH010000000
VERSION     DBNGOH010000050.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6044)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6044)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6044
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3490292"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(249..1412)
                     /locus_tag="AC159P_01010"
     CDS             complement(249..1412)
                     /locus_tag="AC159P_01010"
                     /inference="COORDINATES: protein motif:HMM:NF024907.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP repeat domain-containing protein"
                     /protein_id="HIR3694058.1"
                     /translation="MNKLIASSVIWALAVLISSCPAQHRRGAQSRDPLFTPAPGSPLD
                     IGSRPNDIAVGDLNRDGRLDVATCNDGATATVLLGDGRGGFTPAPSSPINVAAHLIVA
                     GDVNNDRNPDLALTHHDSFGVVILLGAGDGRFAPAPGSPFAAHQGVKPHNHGLALSDL
                     NSDGNLDVTTSNQDDNSVSVLLGDGRGGFAPAAGSPFAVGRAPYPHSTGDLNGDGRLD
                     IVTPNVGGNSVTVLLGDGRGGFTPAAHSPYAVATRPYYIAIGDVSGDGKPDLITTHDD
                     ISLMTTLLGDGRGRFTAAPASPFDLGRRAYKLVAADIDRDTRVDLVIGTEASDSVAVL
                     LSNERGGYTPAAGSPYTAGRNPRIALGDVNGDGRPDIITANNGSGDITVLLGK"
     gene            complement(1700..2815)
                     /locus_tag="AC159P_01015"
     CDS             complement(1700..2815)
                     /locus_tag="AC159P_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HIR3694059.1"
                     /translation="MKAIAVTPGVPDSVRLVEMPTPEVSDVANGRGVLVRVLRVGVDG
                     TDKEINAAEYGAAPEGFDFLVIGHEGFGQVEAVGPNVTEVAPGDFVVATVRRPGGSIY
                     DLIGTNDMTTDDTYYERGINLRHGFMSEYYVDDAEFVVKVPRGLEEVGVLLEPLTVVE
                     KGVAQAYEIQRRLRVWRPRRAAVMGAGTIGLLATLALRLRGLEVVTFGKTPKPYLNAE
                     LIEALGATYVTSDELPVAESARRYEGGFDLIFEATGYSPIVFESMRALAKNGVLVLSS
                     VTGGDRRVEVPADKLNLEFVLGNKVMVGTVNANREYFEAGVRDMSQAELAYPGWLARL
                     LTHPIRGLESYRELFDKLTKAEGAIKVYCEVAEPAAK"
     gene            complement(2856..3344)
                     /locus_tag="AC159P_01020"
     CDS             complement(2856..3344)
                     /locus_tag="AC159P_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007669038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing (seleno)protein"
                     /protein_id="HIR3694060.1"
                     /translation="MKLRKTLPVVAVVIGLAAAIGAFGLRRADGIDPVNKTSSGLALR
                     GYDAVAYFREGGPAEGHAEFAHEWNGARWLFRNAEHRAAFAREPEAYAPQYGGYCSYA
                     VAHGYTADADPRAWKVVNGKLYLNYNEEVRQLWEQDIRGYLRKSEMNWPQFLEHKPEH
                     KG"
     gene            complement(3489..5396)
                     /locus_tag="AC159P_01025"
                     /pseudo
     CDS             complement(3489..5396)
                     /locus_tag="AC159P_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016859933.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MGH1-like glycoside hydrolase domain-containing
                     protein"
     gene            complement(5678..5983)
                     /locus_tag="AC159P_01030"
                     /pseudo
     CDS             complement(5678..5983)
                     /locus_tag="AC159P_01030"
                     /inference="COORDINATES: protein motif:HMM:NF012935.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
BASE COUNT          993 a         1966 c         2004 g         1081 t
ORIGIN      
        1 ggcagttcga cacatgggcg gtgatggtga gaaaaggggc gagagtaccg cgggggtggg
       61 atggagtgct ggccggatga gcctggatat gtggggtgag gtgcttgcgc gcgccggctc
      121 tactttccgc accagaacca acacgactac agcatcgcct tgtactgccg cagcatctcc
      181 ctcgcctccc cgtcggttac ctcttcctaa cctcggtcac tctatcgatt ctcgcaaaat
      241 tacccggact acttacctaa caggacggtg atatcaccag acccgttatt ggccgtgatg
      301 atgtcgggcc tgccgtcgcc gttcacatcg cccagagcta ttctcggatt cctgccggcc
      361 gtgtacggag agcccgcggc aggcgtgtaa ccgccccgct cattactcaa caacacggcc
      421 acggaatcgc tcgcctcggt cccgatgaca agatccacac gggtgtctct gtcgatgtcg
      481 gcagcgacaa gcttgtaagc gcgccgccca agatcgaacg gcgaggccgg cgcggctgtg
      541 aaccgtccgc gaccgtcccc caataaggtg gtcatcaggc tgatgtcgtc gtgggtcgta
      601 atcaagtcgg gcttgccgtc cccgctcacg tctccgatgg cgatgtaata cgggcgggtc
      661 gcgaccgcgt acggcgaatg cgccgcgggc gtgaagccgc ctctgccgtc gcccaacaga
      721 acggtcacgc tgttgccgcc gacattgggg gtcacgatgt cgaggcgtcc gtccccgttc
      781 aggtcgccgg tggaatgagg gtagggcgcc ctcccgacag cgaagggtga acccgccgcg
      841 ggcgcgaagc cgcccctgcc gtcgcccagt aagaccgaga cactgttgtc gtcctggtta
      901 gaggtggtga cgtcgagatt cccgtccgag ttcaagtcgc tgagggcgag gccgtggttg
      961 tgtggcttga cgccctgatg tgcggcgaaa ggcgagccgg gtgcgggggc gaatctgccg
     1021 tcacccgcgc ctaataggat caccacgccg aagctgtcat ggtgcgtgag cgcgaggtcc
     1081 gggttgcggt cgttgttgac gtcgccggcc acgatcaagt gtgcggcgac attgatcggc
     1141 gagctcggcg cgggcgtaaa accgccccgc ccgtcgccca gcaggaccgt cgccgtagcc
     1201 ccatcgttac aggtggcgac atccagcctc ccgtctctgt tcaaatcgcc gacggcgatg
     1261 tcattgggcc ggctgccgat atcgagtggg gagcccgggg cgggcgtgaa gagggggtct
     1321 cgggattggg cgccgcgccg gtgttgtgcc ggacaactcg atatcagaac cgccagcgcc
     1381 catatcaccg aagaagcaat cagcttgttc atgcgcgacc tttccgtgga cggcgtggcc
     1441 cgtcacttca gagttcggac gttagacgga gatgtcggtc ttttccttcc atagttgtcg
     1501 gtcgagtgct ttgcctcacg attcgaagcg gctaccaaaa ggtgggtgag tcgagctcgc
     1561 gtaagtccga aattttgtcg gtctgcctga atcgtgaggc aaagcacctc gccatacaac
     1621 cttcgcagtg aagagatggg gggacccggt agggtccccc gcgaagccga tcgtagtcgg
     1681 acggcagttt tttccagcct tacttcgcgg ctggctcggc gacctcgcag tagactttaa
     1741 tggcgccctc ggccttcgtc agcttgtcga acagctcacg gtagctctcc aacccccgga
     1801 tcgggtgtgt gagcagccgc gcgagccagc cggggtatgc cagctctgcc tgggacatgt
     1861 cgcgcacgcc cgcctcgaag tactcgcggt tggcgttgac ggtgccgacc atcaccttgt
     1921 tccccaggac gaattcgagg ttgagcttgt cggcgggcac ctcgacgcgg cggtcgccgc
     1981 ccgtgacgct ggagaggacg agcacgccgt tcttggcgag cgcgcgcatc gactcgaaga
     2041 cgatgggcga gtagccggtc gcctcgaaga tgaggtcgaa gccgccctcg taacgtctcg
     2101 ccgactcggc cacgggcagc tcgtcgctcg tgacgtaggt cgcgcccagc gcctcgatca
     2161 gctcggcatt gaggtagggc ttcggcgtct tgccgaaggt gacgacttca agcccgcgca
     2221 gccggagcgc gagcgtggcg agcaacccga tggtgccggc gcccatcacg gctgcgcggc
     2281 ggggccgcca cacgcgcagg cgccgctgta tctcgtaagc ctgcgcgacg cccttctcga
     2341 ccaccgtcag aggctccagc agcacgccga cctcttcgag tccccgcggc accttgacga
     2401 cgaactcggc gtcgtccacg tagtactccg acatgaagcc gtggcggagg ttgatgccgc
     2461 gctcgtagta cgtgtcgtcg gtcgtcatgt cgttcgtgcc gatcaagtca tagatgctcc
     2521 cgcccggacg cctgacggtg gcaacgacga agtcgccggg ggcgacctcg gtaacgttcg
     2581 gcccgacggc ctcgacctgc ccgaagccct cgtggccgat gacgaggaag tcgaaaccct
     2641 cgggtgcggc gccgtactcg gcggcgttga tctccttgtc ggtgccgtcc acgccgaccc
     2701 tcagcacgcg caccagcacg ccgcgcccgt tcgctacgtc cgagacctcg ggcgtcggca
     2761 tctcgacgag gcgcacgctg tcgggcacgc ccggtgtgac cgctatcgct ttcatcacga
     2821 attctcccgt ccttcggctg aagtgttcga gacagttaac ccttgtgctc gggcttgtgt
     2881 tcgaggaact gcggccagtt catctcgctc ttccgtaagt agccgcggat gtcctgctcc
     2941 cagagctgcc tcacttcctc gttgtagttg aggtagagct tgccgttgac aaccttccag
     3001 gcgcgcgggt cggcgtcggc agtatagccg tgggcgacgg cgtaagagca gtagccgccg
     3061 tactgcggcg cgtaagcctc cggctcgcgc gcgaaggcgg cgcggtgctc ggcgttcctg
     3121 aacaaccagc gcgcgccgtt ccactcgtgc gcgaactcgg cgtgcccctc ggctggcccg
     3181 ccctcgcgga agtaggccac cgcgtcgtag ccgcgcagcg ccagcccgga cgaagtcttg
     3241 ttgaccgggt cgatgccgtc cgcgcgtctc aagccgaacg cgccgatggc cgcggcgagg
     3301 ccgatgacga ccgcgacgac cgggagtgtc tttctgagct tcacaatgtc cctcctcatg
     3361 aaatcgaacc accgcgacat gaaaagtcgc ctcaccgaat tagtcgggcg gcgccgcgaa
     3421 accttacgga agaagtgatg agtgatgaat cttgccgcat ggccgtatcc atcactcatc
     3481 actcgtcttc actcgccgct ctgttgcagc agcttggcga cgaggcccgt ccagccggtc
     3541 tggtgcgagg cgccgacgcc cgcgccggtg tcgccgtgga agtactcgta gaagagcacg
     3601 aggtcgcgcc agtgcgggtc gcgctggaac ttctccgagc cgccgtagac gggccgccgg
     3661 ccctctccgt cgcgcaggaa gatgtgcgac aggcgacgcg agagttccgc cgcgacctca
     3721 ccgagcgtca tctccacgcc cgagcccgcg gggcattcga cgcggaagtc ggggcctaag
     3781 tagtggtgga acttctgcaa ggactcgatc aagaggtagt tgaccgggaa ccagacgggg
     3841 ccgcgccagt tcgagttgcc gccgaacagc ccggtcgatg attctcccgg ctcgtaatcg
     3901 acgcggtgct cggtgccgcc gacgtgcagc acgtaagggt gctcgcggtg gcggcgcgag
     3961 acggcgcgga tgccgtgcgg cgaaaggaac tcagactcgt cgagcatcac gcgcaggacg
     4021 cggcgcagtc gctcgcgatc cacgacggcg agcaggcgtc gctcgccgct ccccttcgtg
     4081 cgcatgcagg ccacgttgcc cgtcaggtcg ggccggttct cgacgaacca ctccatgcgg
     4141 cgcttgaagc cggggagaag ttcgaccgtc tccggctcaa gcgtctcgac ggcgaagagc
     4201 gggacaagcc ccaccatcga gcgcaccttc atcggcatgc tcccctgccc cggcaggtgc
     4261 agcacgtcgt agtagaaccc ctctcccttg tcccacaaag agatgtcgtc ctcgcccagg
     4321 ttgttcatcg cgtgggcgat gtagaggaag tgctcccaga acttcgaggc cacgtcctcg
     4381 tacgccgggt ccgtcttcgc cagctccaac gcgatggcca gcaggttgag cgtgtacatc
     4441 gccatccagc tcgtgccgtc ggactgcgcg aggtggccgc ccgtggggag cggctgcgag
     4501 cggtcgaaga cgccgacgtt gtccatcccg aggaagccgc cctggaagat gttcatgcct
     4561 tcggcgtcct tgcggttgac ccaccacgtg aagttcaaca gcagtttgtg gaagacgcgc
     4621 tggaggaagt ccctgtctcc gatgccgcgg cgctttttct cgatcttgta gacgcgcagg
     4681 gcggcccacg cgtggacggg tgggttcacg tcgccgaagg cccactcata cgcggggagc
     4741 tgtcccgagg ggtgcatgta ccactcgcgg agcatgaggg tcagttgctc cttcgcgaag
     4801 tcggagtcca cgagcgcaag cgggacgcag tggaaggcca agtcccagac ggcgtaccac
     4861 gggtactccc acttgtcggg catcgagatc acgtcggcgt tgtacatgtg cgcccactcg
     4921 cggttgcggc ctcgttcgcg ctcggctggc ggcgcgggct gcgccgggtc gcccttgagc
     4981 cagtcgcgca cgacatagtg gtagaactgc ttcgaccaga gtagtccggc gagcgactgc
     5041 cgcatgacgc cgcgcgcatc ttcggagagg tctgcgggca cgaccgtctc atagaactcg
     5101 tccgcttccc gcctcctcgc ctcgaaggtg cggtcgaact cttcgccgaa ccggttcgcg
     5161 cccggctgta cgtcggtcag acgcaggcgt acggtcgccg tctcgcccgg cgctacgtcg
     5221 agcgcgtagt gagccgcgac cttcgtgccg cggcgctcgg ggttcaccgc ctcgcggttg
     5281 ccttggacga gatagttgtt gacgccgtcc ttgacgtaag acctgttcgg cacgccgtag
     5341 agccgctcgg tgtttgtctc gttctcggtg aagagcagct cgggcgcgct ctcgcagtga
     5401 agtgcctggc catcaagtcg agcgtgaagc ccggcgcgat cttcggcgcc ccggctgaga
     5461 gcgccgcccc gtcgagcttg ccgacgaagg agatgtgccc catcgggaag tcgtaagtct
     5521 cgtcgccgaa gtagaaatcg ttgacggcaa gcgtcttctg gaagacggtc gggttcgggc
     5581 acttcgagac ggcgagcacg accgagttga cgtgccccat gtagtggcgt ccgaccgtgt
     5641 cggacgagtt ggccaagccc cgcgggtgtc tgtcgctcgc ggagcgtagc agcagcgccg
     5701 ccgagttgat ggcgccgcac gagacggcga cgaagtccgc cgagaacgtc tcgcgctcgc
     5761 cgccgtcgcg ctccacgtga acccgcgtga cgacgcggcc cgaggcgtcc gtctcaagcc
     5821 gcgtgacttt ggcgccggtc aggagcgtga cgttcgtgtg ttcgagcgcg ggatcgacgg
     5881 cgcagacgtg cgcgtccgcc ttggcctgca cgagacacgg gaagccgtcg cacgtcccgc
     5941 agcggataca cttgctgcgc cgcgggtcgt cctccttgag catcacgccg agcgggacgt
     6001 ggaagggccg gcacccgagc cgcgcgaagt cttcggcgag ccgg
//
LOCUS       DBNGOH010000051         4118 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1474027, whole genome shotgun sequence.
ACCESSION   DBNGOH010000051 DBNGOH010000000
VERSION     DBNGOH010000051.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4118)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4118)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4118
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1474027"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..293
                     /locus_tag="AC159P_01035"
     CDS             <1..293
                     /locus_tag="AC159P_01035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694061.1"
                     /translation="APAGRPADSPDKAALELADRLHAVHFFCPEGGAYRVAPDGKSVS
                     CDVHGTASAPRQPPAPTARSAAGRLMNALSDATATLTFMEDGLHATVVVNRK"
     gene            615..1130
                     /locus_tag="AC159P_01040"
     CDS             615..1130
                     /locus_tag="AC159P_01040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694062.1"
                     /translation="MPDDSNDIIIKFGTNANKQDVTDFSLDVLRDILRAAGLKSALIS
                     STARNPANQARVMYNNLVARGVAHQKKLYAAAGDAVIDVYAASKAAGKNAAQIKADME
                     AKINEVGPTRVSRHAADPKVLCVFDVAPSSISNKDAFESAVEADTRVAKFLTPPGDPG
                     YHLEIPQPPAS"
     gene            complement(1253..1999)
                     /gene="nfi"
                     /locus_tag="AC159P_01045"
     CDS             complement(1253..1999)
                     /gene="nfi"
                     /locus_tag="AC159P_01045"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012930596.1"
                     /note="cleaves DNA at apurinic or apyrimidinic sites;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HIR3694063.1"
                     /translation="MARYEQLHEWSLTPREAVELQKKLRERVRVAPLRKKIETVAGAD
                     ISFNKFDPVVYAAVVVLRLPSLEVVEEAGVVSETKFPYVPGLLSFRETPSVLEAWARL
                     KTEPDAVMFDGQGLAHPRRVGIASHVGLLIDRPTLGCAKSVLVGKYEEPAPERGAWTP
                     LVDKGETVGAALRTKTRVQPIYVSPGHLIDLAGAIDLTLRSDGGYRQPEPTRRAHLFV
                     NALRRGERAPTSDDDGANGAASEGQGSFDF"
     gene            complement(2102..3712)
                     /gene="nadB"
                     /locus_tag="AC159P_01050"
     CDS             complement(2102..3712)
                     /gene="nadB"
                     /locus_tag="AC159P_01050"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HIR3694064.1"
                     /translation="MSIESDYIVVGSGIAGLRAAIELARAGARVTVLTKDRTSESNTE
                     YAQGGVAVALSEDDEASLHEEDTLEAGAGLCDERAVSVLVEDGQKYIQELIGWGAEFD
                     REGGRLLFTREAAHSRARILHAHGDATGKEIVRALIARAGAERGITFIAHAATEELIV
                     EDGGVRGVRYLDPLVRAPRSLRARGVVLASGGAGALYLHTTNPSVATGDGMAMAYAAG
                     AELADMEFVQFHPTALAVEGAPRFLLSEALRGEGGVLRNEAGKRFMPRYHERAELAPR
                     DIVSRSIVAEMERTATRAVFLDMTAFDARYLRRRFPKIHATCLQFGIDLAREAVPVSP
                     AAHYVMGGVRTDLDGRTTVRGLYAAGEVACTGVHGANRLASNSLLEGLVFGARAGRAA
                     AEDLEGAGAAPDGGAGREAREGEREASPEELGRMGAAALAVRKRVRRLMWERVGILRS
                     RASLQRALSEFEQIARAHLRPGPRNFLTVATLVARSALWREESRGAHHRVDFPARDDE
                     RWRVHSIIQKDAGISSSETVEFNAAVTL"
     gene            complement(3724..3951)
                     /locus_tag="AC159P_01055"
     CDS             complement(3724..3951)
                     /locus_tag="AC159P_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002764763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694065.1"
                     /translation="MMKARQEQDDEMRPEYDLSQLKGRVRGKYVERYRAGTNHVLLDP
                     DVAAAFPDAKAVNDALRMLIEVARKQVSEQS"
BASE COUNT          645 a         1539 c         1295 g          639 t
ORIGIN      
        1 acgcccccgc gggccggccc gcggactctc ccgacaaggc cgcgctcgaa ctcgccgacc
       61 gccttcacgc cgtccacttc ttctgcccag agggcggcgc ctaccgcgtc gcccccgacg
      121 gcaagtccgt ctcctgcgac gtgcacggca cggcctccgc gccgcgccag cccccggccc
      181 ccaccgcgcg cagtgccgcg ggccgactca tgaacgcgct ctccgacgcg accgccacac
      241 tgaccttcat ggaggacggg ctgcacgcga cggtcgtcgt caacaggaag tagagcgcgc
      301 cgcgaccgcg cgccgcatcc agacgatgtg cgcttgagtg taccgtgcgt tcatttcaac
      361 acagagggca cggaggtctc acagagagca cggagaaaac cacttcttct ccgtgccctc
      421 cgtggaaact ctgtgaactc cgtgtcgaat cgaacccgcg ttgaactcaa ccgcgattcg
      481 atccagagcg tgtcgggatg cctcccgccg cgcgcgcccg ctggcggtag gcgcggcggg
      541 gttagttgtg gtatgaagtg cgtcgctcta cctgatgccc ttgagtcctc aagtcaccga
      601 agggagaatc gatcatgccg gacgactcga acgacatcat catcaaattc ggcaccaacg
      661 ccaacaagca ggacgtgacg gacttttcgc tcgacgtgct cagagacatc ctccgcgccg
      721 ccggcctgaa gagcgcgctc atctccagca ccgcgcgcaa cccggccaac caggcgcgcg
      781 tcatgtacaa caacctcgtc gccagaggcg tcgcgcacca gaagaagctg tacgccgccg
      841 cgggcgacgc ggtgatcgac gtgtacgccg cctcgaaggc cgccgggaag aacgccgcgc
      901 agatcaaggc cgacatggag gcgaagatca acgaggtggg gccgacccgc gtctcgcgcc
      961 acgccgccga cccgaaggtg ctctgcgtct tcgacgtggc gccctcctcc atcagcaaca
     1021 aggacgcctt tgaatcggcg gtcgaggccg acacgcgcgt ggcgaagttc ttgaccccgc
     1081 ccggcgaccc cggctaccac cttgagatac cgcagccgcc cgcgtcgtaa tcgcggaggg
     1141 gaaacggaga ggggaagagg ggaagaggaa gagaaagcaa ctcgcctgtc ttccttttcg
     1201 cctctccccc ttttaacctt ttcccccttc cttttaccct tttcccctct ccttaaaagt
     1261 cgaagctgcc ctgcccctcg ctcgcagctc cgttcgcgcc atcgtcgtcc gaggtcggtg
     1321 cgcgctcgcc gcggcgcagc gcgttgacga agaggtgcgc gcggcgcgtc ggctcgggct
     1381 ggcggtagcc gccgtcgctg cggagcgtga ggtcgatcgc gcccgcgagg tcgatgaggt
     1441 ggccgggcga gacgtagatg ggctgcacgc gcgtcttcgt ccgcagggcc gcgccgaccg
     1501 tctcgccctt gtccacgagc ggcgtccacg cgccgcgctc cggcgcaggc tcctcgtact
     1561 tccccaccag cacggacttc gcgcagccga gcgtcgggcg gtcgataagc aacccgacgt
     1621 gcgacgcgat gccgacgcgg cgcgggtgcg cgaggccctg cccgtcgaac atcaccgcgt
     1681 ccggctcggt cttaagccgt gcccacgctt ccagcaccga cggcgtctcg cggaacgaga
     1741 gcagccccgg cacgtacggg aacttcgtct cgctcacgac gcccgcctcc tcgaccactt
     1801 cgagcgacgg caggcgcagc acgaccaccg ccgcgtacac caccgggtcg aacttgttga
     1861 aagagatgtc ggcgcccgcc accgtctcga tcttctttcg cagcggcgcg acgcgcacgc
     1921 gctcgcgcaa cttcttctga agctcgaccg cctcgcgcgg cgtcagcgac cactcgtgca
     1981 gttgttcgta tcgggccatg agtaataatt gtaggagcag ggcaagtccg cggcccttca
     2041 actttcgatg aattcaacgt tgaatgaaga cgtgagcgcg ggccgaggac aagccctgcc
     2101 cctacagtgt taccgcggcg ttgaactcca ccgtctcgct tgaagagatg cccgcgtcct
     2161 tctgaatgat ggagtggacg cgccagcgct cgtcgtcgcg cgcggggaag tccacgcggt
     2221 ggtgcgcgcc gcgcgactct tcgcgccaga gggcggagcg cgcgacgagc gtcgccacgg
     2281 tcaggaagtt gcgcgggccg gggcgaaggt gcgcgcgcgc gatctgctcg aactcggaga
     2341 gcgcgcgctg tagcgacgcg cgcgagcgca ggatgccgac gcgctcccac atcaaacgcc
     2401 ggacgcgctt gcgcaccgcc agcgccgccg cgcccatgcg gcctagttct tccggcgacg
     2461 cctcgcgctc tccctctcgc gcctcgcgcc ccgccccgcc gtccggcgcg gcgcccgcgc
     2521 cctccaagtc ttccgccgcc gcgcgccccg cccgcgcgcc gaagacgaga ccttccagca
     2581 gcgagttcga cgccagccgg ttcgcgccgt gcacgcccgt acacgcgacc tcgcccgccg
     2641 cgtagaggcc gcgcacggtc gtccgcccgt cgaggtccgt gcgcacgccg cccatcacgt
     2701 agtgcgccgc cggggagacg gggacggcct cgcgcgccag gtcgatgccg aactgcaaac
     2761 aggtcgcgtg gattttcggg aagcggcggc gcaggtatcg cgcgtcgaag gccgtcatgt
     2821 cgaggaagac ggcgcgcgtt gccgtgcgct ccatctccgc gacgatggag cggctgacga
     2881 tgtcgcgcgg cgccagctcc gcacgctcgt ggtagcgcgg catgaagcgc ttgcccgcct
     2941 cgttgcgcag gacgccgccc tcgccgcgca gagcttcgga cagcaggaag cgcggcgccc
     3001 cctcgacggc gagggccgtc gggtggaact gcacgaactc catgtcggcc agctccgccc
     3061 ccgccgcgta ggccatcgcc atcccgtcgc cggtcgccac gctcgggttc gtcgtgtgca
     3121 ggtagagcgc cccggcgccg cccgacgcga gcaccacgcc gcgcgcgcgc agagagcgcg
     3181 gcgcgcgcac cagcgggtcg aggtagcgca cgccgcgcac gcccccgtct tcgacgatca
     3241 actcttcggt cgccgcgtgc gcgatgaacg tgatgccgcg ctcggccccc gcgcgcgcga
     3301 tgagtgcgcg gacgatctcc ttgccggtcg cgtcgccgtg cgcgtgcagg atgcgcgcgc
     3361 gcgagtgcgc ggcctcgcgc gtgaacagca ggcgcccgcc ctcgcggtcg aactccgcgc
     3421 cccagccgat cagctcctgt atgtacttct gcccgtcctc gaccagcacc gacaccgcgc
     3481 gctcgtcgca cagcccggcg cccgcctcca gcgtgtcctc ttcgtgcagc gacgcctcgt
     3541 cgtcttccga cagcgccacc gccacgccgc cctgcgcgta ctccgtgttc gactcgctcg
     3601 tgcgatcctt cgtcagcacc gtcacgcgcg cccccgcgcg cgccagctcg atcgccgcgc
     3661 gcaaccccgc gatgccgctg ccgacgacga tgtaatcgga ttcgatggac atgacctttg
     3721 aacttaactt tgctccgaga cctgtttgcg cgcgacttcg atgagcatgc gcagcgcatc
     3781 gttcaccgcc ttcgcgtccg ggaacgccgc cgccacgtcc gggtcgagca ggacgtggtt
     3841 cgtgcccgcc cggtaacgct ccacgtattt gccgcgcacc ctgcccttca actgcgaaag
     3901 gtcatactcg gggcgcattt cgtcatcctg ctcctgccga gctttcatca ttactgcact
     3961 ctctctaaaa gttgaatgac ctctgcgccg aagtttatca cagacgtttg cgctgcctgg
     4021 ccgacgcccg tcgaactgtg ttgcgcccct cggcggcccg cgctaaaatc gcggcgcgga
     4081 ttcatcccgt tccccggaca aaatccagtc gagagtgc
//
LOCUS       DBNGOH010000052         2586 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2390892, whole genome shotgun sequence.
ACCESSION   DBNGOH010000052 DBNGOH010000000
VERSION     DBNGOH010000052.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2586)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2586)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2586
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2390892"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1078)
                     /locus_tag="AC159P_01060"
     CDS             complement(<1..1078)
                     /locus_tag="AC159P_01060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HIR3694066.1"
                     /translation="MTDRHPLIAATVRGTLVLSLVLSLSASALASNGARDAKAESRPV
                     AKTVRATTDGAATLITIAGTAPMAYAVRRADARLLVVELPGVDASQLAPSYQVSSPLV
                     SGVTVKLAVKENNVAVAGVQVALRVPVLERSRMEGNALVLELRPEQASGDVAAADKVN
                     AADKSGAAQPQQSSSAQNGPQTAQGKMYGQPGFVGEPINLNVVNADIRDILNYITEQY
                     GINFVVDSSVQRVPVTINVTEVPWNFALDSILKANRLGVEVTGNILRIATSATIADEA
                     RIRATEEGARARLREAQLDNAPLVTEFIRLNYARASGTLAGAAGSTGGFSGGSTPQSF
                     TGGAGGDALGSSGDTGIMPIIGKRL"
     gene            complement(1352..2077)
                     /locus_tag="AC159P_01065"
     CDS             complement(1352..2077)
                     /locus_tag="AC159P_01065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694067.1"
                     /translation="MRERNRIIRHALFALAAPLVLGLGTGAGAQTASTTYKGMRHDPF
                     TKYKPPVKRLVEKKAPGVVAVPPIQTRIDNYKAQRVVAMSQQRPAPKPTTALLIGEVQ
                     VNGIFRTPRGYAAMVEATPIRLSYVIYPGEAFYDGMLVAIEETKLVFRRETRWTDGRR
                     EVAVEIKPLRQASVDEAMTAKTAAPANAAPPPAQTGGTGQVPGAAVAVSDDTTPTKDG
                     ANSKAVENLNSKVDKLIELLKKQ"
     gene            complement(2112..>2586)
                     /locus_tag="AC159P_01070"
     CDS             complement(2112..>2586)
                     /locus_tag="AC159P_01070"
                     /inference="COORDINATES: protein motif:HMM:NF016253.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type IV pilus inner membrane component PilO"
                     /protein_id="HIR3694068.1"
                     /translation="IVASQRLNEFKANYERAKADYEDLKLLLPEQRELTMVLQNVQDR
                     ARGRLTLNKFEPKDEVQQDFYTGKPVQVVVTGNYNNIGQFFAQMASYQRIVSITDFKI
                     MRLKGNRLKDVTEGRTVDAQFLLTAYYVSPEKLQTAAPAAKAQPAAPAAPAAPAK"
BASE COUNT          328 a          845 c          931 g          482 t
ORIGIN      
        1 acaggcgctt gccgatgatc ggcatgatgc ccgtgtcgcc gctgctgccg agcgcgtcgc
       61 cgcccgcgcc gcccgtgaaa ctctgcggcg tcgagccgcc tgagaagccg ccggtcgaac
      121 ccgccgcccc ggcgagcgtg cccgaggcgc gcgcgtagtt aaggcggatg aactcggtga
      181 cgagcggcgc gttgtcgagc tgcgcctcgc gcaggcgggc gcgcgcgccc tcttcggtcg
      241 cgcggatgcg cgcctcgtcg gcgatggtcg cgctcgtggc gatgcggagg atgttgcccg
      301 tcacctcgac gccgaggcgg ttcgccttga ggatcgagtc gagtgcgaag ttccacggca
      361 cctccgtgac gttgatcgtg acgggcacgc gctgcacgga cgaatcgacg acgaagttga
      421 tgccgtactg ctcggtgatg tagttcagga tgtcgcggat gtcggcgttg acgacgttga
      481 ggttgatcgg ctcgccgacg aagcccggct ggccgtacat cttgccctgc gcggtctgcg
      541 gcccgttctg tgcggacgaa ctctgctgcg gctgcgcggc gccggacttg tccgcggcgt
      601 taaccttgtc cgccgccgcc acgtcgcccg acgcctgctc gggcctgagt tcgagcacca
      661 gcgcgttgcc ttccatgcgc gagcgttcga ggacgggcac gcgcaaagcc acctgcacgc
      721 cggcgaccgc gacgttgttc tccttcacgg ccagcttcac cgtgacgccg gagacgagcg
      781 gcgaagagac ctgataagag ggcgcgagct gcgaggcgtc aacgccgggc agttcgacga
      841 cgagcaggcg cgcgtcggcg cgccggacgg cgtaggccat cggcgcggtg cccgcgatgg
      901 tgatgagcgt cgcggccccg tcggtcgtgg cgcgcacggt cttggccacg ggacggctct
      961 cggccttcgc gtcgcgcgcg ccgttcgagg cgagtgccga cgccgagagc gagagcacga
     1021 gcgagaggac gagcgtgccc ctcaccgtgg ctgctatcag tgggtgtctg tcagtcattt
     1081 cattatcctt cttcggcatc gcccacctgc cgccgccaca cgctgacgct cgttcggctt
     1141 ccccttgagg ggggaagcgt acttcaagcg cgcgccgcac aacgcggaag gccgtgggcc
     1201 atgttgtcaa aagactcgct tgcgagtggc gttgatcgat tctcctcgtg gttcgtgggt
     1261 cgtgtcgccc ggcgtggggt ttcgaggggg gcgtgactct cgggccgggc ggcggcgttc
     1321 ttgaagcggc ggcgcgcccg gcccggcggg actactgctt cttcaacagc tcgatgagct
     1381 tgtccacctt gctgttcaaa ttctcgaccg ccttgctgtt cgcgccatcc ttcgtcggcg
     1441 tcgtgtcgtc cgagaccgcg acagccgcgc cggggacttg acccgtgccg cctgtttggg
     1501 cgggcggcgg ggcggcgttc gcgggcgcgg cggtcttggc cgtcatcgcc tcatcgacgc
     1561 tggcctggcg cagcggcttg atctcgaccg ccacttcgcg ccgaccgtcc gtccagcgcg
     1621 tctcgcgccg gaagacgagc ttcgtctcct cgatggcgac gagcatcccg tcgtagaaag
     1681 cctcgccggg gtagatgacg taggagaggc ggatcggcgt cgcctcgacc atcgcggcgt
     1741 agccgcgcgg cgtgcggaag atgccgttga cctgcacctc gccgatgagc agcgcggtgg
     1801 tcggcttcgg cgccggcctc tgctggctca tcgccaccac gcgctgcgcc ttgtagttgt
     1861 cgatccgcgt ctggatgggc gggacggcga ccacccccgg cgccttcttc tcgacgaggc
     1921 gcttgaccgg cggcttgtac ttcgtgaagg ggtcgtggcg catccccttg taggtcgtgc
     1981 tcgccgtctg cgcgccggcg cccgtgccga ggccgaggac gagcggcgcg gcgagcgcga
     2041 acagggcgtg gcggatgatt cggtttctct ctctcatatg ctttcccttg cgtgcggtcg
     2101 cgccgcggtt cctacttcgc gggcgcggcg ggcgcggcgg gcgccgcggg ctgggccttg
     2161 gcggcgggcg ccgcggtctg gagcttttcg ggcgagacgt agtaggccgt caggaggaac
     2221 tgcgcatcga ccgtgcgccc ctcggtcacg tccttgaggc ggttgccctt gaggcgcatg
     2281 atcttgaagt cggtgatcga gacgatgcgc tggtaggagg ccatctgcgc gaagaactgg
     2341 ccgatgttgt tgtagttgcc cgtgaccacg acctgcacgg gcttgcccgt gtagaagtcc
     2401 tgctgcacct cgtccttcgg ttcgaacttg ttgagcgtca ggcggccgcg cgcgcgatcc
     2461 tgcacgttct gcaagaccat tgtcagctcg cgctgctcgg gcagcagcag cttcaggtct
     2521 tcgtagtcgg ccttggcgcg ctcgtagttg gccttgaact cgttgaggcg ctgcgaggcg
     2581 acgatg
//
LOCUS       DBNGOH010000053         3859 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2757559, whole genome shotgun sequence.
ACCESSION   DBNGOH010000053 DBNGOH010000000
VERSION     DBNGOH010000053.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3859)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3859)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3859
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2757559"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1312)
                     /locus_tag="AC159P_01075"
     CDS             complement(<1..1312)
                     /locus_tag="AC159P_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015352689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HIR3694069.1"
                     /translation="MSLTLLAIGFVALLVVGYRLYGGWVARQFRLDDGRRTPAHEVND
                     GVDFVPTKPFYLFGQHFSAIAAAGPIAGPILACQAFGWLPCLLWIGLGVVLIGAVHDF
                     TSLAASVRHGATSVAEITREHLGNKAGRAMMAFIWIALIYVIVAFADITAGTFVGGSE
                     ELKAAAVNFNPGGAVAAASVLYLLLSVVLGLVQRYLNPPLWLVTLIFVPAAFALSWVG
                     TQMSTTFVMSHKAWGVLILVYCCVASMVPVWALLQPRGYLGGFILYAAIALGIIGVFF
                     GGYEIRQPAFKSWDVGGMTGMLFPFLFVTIACGACSGFHGLVCSGTTSKQVERESHIR
                     PVGYGAMLAEGFVALIALVTVMIAAEGALKGKAPGTIYGGGIGQFLTLIIGRENLAVA
                     TTFGAMAFSTFVFDTLDVSTRLGRYIVQELLGSRGRAGAVAGTLL"
     gene            complement(1541..3136)
                     /locus_tag="AC159P_01080"
     CDS             complement(1541..3136)
                     /locus_tag="AC159P_01080"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013739.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0016116 - carotenoid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HIR3694070.1"
                     /translation="MRDRVVIVGAGHNGLVAACYLARAGFAPLVLERREVVGGACVTE
                     EFHEGFRCSTLAHSAGPLLARVSSELGLEQHGLAFIRPATSVFAPAPDGGPSLRVYKD
                     AARTAREIEKLSARDAAKYAEFQESFARIGRVLAPLLSATPPSIDRPGAADVWNFGRL
                     GKDFRGLGKRDAHRLLRWGPMAVADLVAEWFETELLRATVAARGIYGAFAGPWSAGTS
                     VPLLLQAAFGGSAVAPASFVRGGMGALTQALASAAKAAGAEVRTGAAVARISVEDGAA
                     TGVVLEDGEEIRARCVVSNADPRRTFLGLVDPVELDPDFLSKVRGYRSRGTAAKLNLA
                     LDALPDFTALKDSRAEATAALSGRIHIGPDIDYLERAFDAAKYGDFSPAPYMDIVIPT
                     LTDPSLAPEGKHVMSVYAQFAPYELKQGDWPSRTEEFANAVVDTLSLYAPNLRDLIID
                     GQLLTPHDLEREYGLTGGHPHHGEMSLDQFYTFRPVIGWAQYRTPLKGLYLCGAGTHP
                     GGGVTGAPGANASREILKDLKGK"
     gene            complement(3275..3814)
                     /locus_tag="AC159P_01085"
     CDS             complement(3275..3814)
                     /locus_tag="AC159P_01085"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0016116 - carotenoid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HIR3694071.1"
                     /translation="MRGAVSISPSIDYMERAYDDAKYGRYSRHPYVDIVIPSLTDPSV
                     APPGKHVMSCFVQYAPYHLKEGHWDERREEFGDAVIDAIAEHAPNIKDIILHRQVLTP
                     LDIERVFGLSEGNIFQGELTLEQLFFLRPAPGWAQYRTPVRNLYMCGSATHPGGGIMG
                     ASGRNAASKILKDWSWGKI"
BASE COUNT          690 a         1281 c         1291 g          597 t
ORIGIN      
        1 tcaaaagcgt gcccgcgacg gcgccggcgc gccccctcga ccccagaagc tcctgcacga
       61 tgtagcggcc gaggcgcgtg ctgacgtcga gcgtgtcgaa gacgaaggtc gagaaggcca
      121 tcgcgccgaa cgtcgtggcg acggcgagat tctcccgccc gatgatgagc gtgaggaact
      181 gcccgatgcc gccgccgtag atggtgcccg gagccttgcc cttcaaggct ccttcggcgg
      241 cgatcatcac ggtcacgagc gcgatcagcg cgacgaaccc ctcggccagc atcgcgccgt
      301 agccgacggg ccgaatgtgc gactcgcgct cgacctgctt cgaggtcgtg cccgagcaga
      361 ccagcccgtg gaagccggag cacgcgccgc acgcgatggt gacgaagagg aagggaaaca
      421 gcatccccgt catgccgccc acgtcccaag acttgaaggc gggctgcctg atttcgtagc
      481 cgccgaagaa gacgccgatg atgccgagcg cgatggccgc gtagaggatg aacccgccga
      541 ggtagccgcg cggctgcaag agcgcccaca ccggcaccat cgaggccacg cagcagtaga
      601 ccagtatcag cacgccccac gccttgtgac tcatcacgaa ggtcgtggac atctgcgtcc
      661 ccacccacga gagcgcgaat gccgccggga cgaagatgag cgtcacgagc cacagcggcg
      721 ggttcaagta cctctgcacg aggcccagca cgaccgagag cagcaggtac aacacgctcg
      781 ccgccgcgac cgcgccgccc ggattgaagt ttaccgccgc cgccttcaac tcctcggagc
      841 cgccgacgaa cgtccccgcc gtgatgtccg cgaacgccac gatgacgtag atgagcgcga
      901 tccagatgaa cgccatcatc gcgcgccccg ctttgttccc cagatgctcg cgcgtgatct
      961 ccgccaccga ggtcgcgccg tgacggacgg aggccgcgag gctcgtaaaa tcgtgcaccg
     1021 cgccgatgag caccacgccc aaccctatcc acagcaggca cggcagccac ccgaacgcct
     1081 gacacgcgag gatcggcccc gcaatcggcc ccgccgccgc aatcgccgag aagtgctgcc
     1141 cgaacaggta gaacggcttc gtcggcacga agtccacgcc gtcgttcact tcatgcgcgg
     1201 gcgtccgccg cccgtcgtcc aacctgaact gccgcgccac ccacccgccg tacagacgat
     1261 acccgacgac gagcagcgcc acgaacccga ttgcgagcag agtcagactc aagggtgtta
     1321 cctccacaag aagaggtcag aggtcggaag tcggacggca gtatacagca ggccgtaggc
     1381 ggtaggcagt aggcagtagg cagcaaaagc aaggggcttg ggaggtatag tctcaagccc
     1441 cttgcttttt tactgcctgt cgcacgcaga ggcttgggac tttacctccc gacctttttc
     1501 gctcttctgt ctttgctgcc tactgcctac tgcccgctgc ctacttgcct ttcaggtctt
     1561 tgagaatctc gcggctggcg ttggcgccgg gggcgccggt gacgccgccg ccggggtgcg
     1621 tgccggcgcc gcagaggtag agacctttga ggggcgtgcg gtattgcgcc cagccgatga
     1681 cggggcggaa ggtgtagaac tggtcgagcg acatctcgcc gtggtggggg tggccgccgg
     1741 tgaggccgta ctcgcgttcg aggtcatggg gcgtaaggag ctggccgtcg atgatgaggt
     1801 cgcggaggtt gggcgcgtag agcgaaagtg tatcgacgac ggcgttggcg aactcctccg
     1861 tgcgcgaggg ccagtcgcct tgcttcagct cgtagggcgc gaactgcgcg tagacgctca
     1921 taacgtgttt gccttcgggc gcgagcgaag gatccgtgag cgtggggatg acgatgtcca
     1981 tgtagggcgc gggcgagaag tcgccgtact tggcggcgtc gaaggcgcgt tcgaggtagt
     2041 cgatgtcggg gccgatgtgg atgcgcccgg agagtgcggc ggtcgcttcg gcgcgcgagt
     2101 ctttgagcgc ggtgaagtcg ggcagcgcgt cgagggcgag attgagcttg gccgccgtgc
     2161 cgcgcgagcg gtagccgcgg actttggaga ggaagtcggg gtcgagttcg acaggatcga
     2221 cgaggccgag gaacgtgcgg cgcgggtctg cgttcgagac cacgcagcgg gcgcgtatct
     2281 cttcgccgtc ttcgagcacg acgccggtcg cggcgccgtc ttcgacgctg atgcgcgcga
     2341 ccgcggcgcc cgtgcgcacc tctgcgcccg ccgccttcgc cgcggaggcg agcgcctgcg
     2401 tcaaagcgcc catgccgccg cggacgaacg aggcgggcgc gacggcgctc ccgccgaacg
     2461 cggcctgcaa caggagcggc acgctcgtcc cggctgacca cggccccgcg aacgcgccgt
     2521 agatgccgcg cgcggccacc gtggcgcgca acagctccgt ctcgaaccac tcggccacga
     2581 ggtctgcgac ggccatcggc ccccagcgca gcaggcggtg cgcgtccctc ttgccgaggc
     2641 cgcggaagtc cttgccgagc ctgccgaagt tccacacgtc cgcggcgccc ggcctgtcga
     2701 tcgaaggcgg cgtcgcagag agcagcggcg ccagcacgcg accgatgcgc gcgaagctct
     2761 cctgaaactc cgcatacttc gcggcgtcgc gcgccgacag cttttcgatc tcgcgcgcgg
     2821 tgcgcgccgc gtctttgtag acgcgcagcg aagggccgcc gtcgggcgcg ggcgcgaaga
     2881 cgctcgtcgc agggcggatg aacgccagcc cgtgctgctc cagccccagc tcgctcgaca
     2941 cgcgcgccag cagaggcccc gccgagtgcg ccagcgtcga acagcggaag ccctcgtgaa
     3001 actcctccgt cacgcacgcg ccgccgacca cctcgcgccg ctcaagcacc agcggcgcga
     3061 accccgcccg cgccagataa cacgccgcga ccagaccgtt gtgccccgcc ccgacaataa
     3121 ccactctgtc tctcataaaa gaagtactga gtaccgagtg ctgagtactg agtgaaagca
     3181 gttgatgagt gaagaatcat gagcgatgag tcggagcagc gtctctgtca tcactcttca
     3241 tccatcaatt gctttcactc agtcctcagc acttttagat ttttccccag ctccaatctt
     3301 tcagaatctt cgaggcggcg ttgcggcccg aggcgcccat gatgccgccg ccggggtgtg
     3361 tggccgagcc gcacatgtag aggttacgga cgggcgtgcg gtactgcgcc cagccgggcg
     3421 cgggccgcag gaagaagagt tgttcgagcg tcagctcgcc ctgaaagatg ttgccttcgg
     3481 agaggccgaa cacgcgctcg atgtcgagcg gcgtcaagac ctgccggtgg aggatgatgt
     3541 ctttgatgtt gggcgcgtgc tcggcgatgg cgtcgatcac cgcgtcgccg aactcctcgc
     3601 gccgctcgtc ccaatggcct tctttcaagt ggtaaggcgc gtactgcacg aagcaggaca
     3661 tgacgtgctt gccgggcggc gcgaccgaag ggtctgtcag gctcgggatg acgatgtcca
     3721 cgtacggatg gcgcgagtac cgtccatact tcgcatcgtc atacgcgcgc tccatgtagt
     3781 cgatgcttgg cgagatcgag accgcaccgc gcaagtgcgg tcctggaccc ggaaggcacg
     3841 tgaagctggg caagccatc
//
LOCUS       DBNGOH010000054         3697 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4407286, whole genome shotgun sequence.
ACCESSION   DBNGOH010000054 DBNGOH010000000
VERSION     DBNGOH010000054.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3697)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3697)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3697
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4407286"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(19..3369)
                     /locus_tag="AC159P_01090"
     CDS             complement(19..3369)
                     /locus_tag="AC159P_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR3694072.1"
                     /translation="MQKLAEVCIQRPVFAAMIVLSLVVVGAASFFRLGVDRFPSVDLP
                     TVSVRTSLPGASAEEVETLISQPIEEVVNTVDGIDELRSVSGPGTSLVIATFKLDRDL
                     ETAAQDVRDRVATVVRSLPEDVLPPVVQKFNNDSSPIMTISLSADRSLRELTELGDKL
                     ARPQFERVGGVGEVSVVGGLERAINVIVDADRLAAYQIPITAVQQALDRQNADIPGGN
                     VVTGREELVLRTMGRYTDPRAFNDLVVATINGSAVRLRDVGRVEDTTKEQRSVARLNG
                     VPTVTLQVRRQSGANTIEVINGIKAAMPRVVGQLPTDVKMEIIRDQSRYIEAALHEIE
                     THLVLGSILACLVVLLFMRSWRATVIAGVAIPTSVIATFGMMRALDFTLNSVTMLALV
                     LMVGVVIDDAIVVLENIFRFIEEKKMRPMEAARKATADIGLAVMATTLSLVVIFLPVS
                     FMSSISGRFLYQFGITAAVAIMVSLLVSFTLTPMMSSRLLRVEDAASGGGHGGHGSGH
                     GEGAASRKGFYAWLDRVYTRLLTISMRHRLAVSILAIGVIFSSVPLYRAVRQEFIPSQ
                     VDEAEFEVSVNAPEGTNITAMNEAVEAVEQEVMKTPGVRMVLASAGGGFLGGVNQGDI
                     YVRIAPHEERTVSISRIWRETLNGNPLNAFKGNYAQQDVMQEIRRRMRKYAPLRVGVR
                     NAPSFNFGAGGRTDIDFIFRGPDLIQLVEYAEDLRDRTEKIGGIVDADTSLKLNKPEL
                     RIEIDRERAATLGVNTADIANSLRIMVGGDTEATRYHDTSVNEAYDVQLRVNEGDRND
                     PRTLGNLYVPSARGGLIRLDNLVNIKRETAPSRIDRLDRQRQVSVRASVAPGFALADR
                     LEALRAEAVSMNLPPAYTTAISGRGRELERTFTEFIWAFMLSIIFMYMILASQFESLI
                     HPVTILLSLPLAVPFALFSQWVLGDTLNLYSALGILVLFGVVKKNSILQIDHMNNLRR
                     EGWERGEAIIQANRDRLRPILMTTLALVAGMLPLALGTGPGAEERRSIAVVVIGGQTL
                     SLLLTLLVTPVAYSVFDDLGNTAWWRRGAASFTLIPRRAVGALRAAFARKRRQPEADE
                     PERTSTPRRGESEETVEVEREEVGVGGD"
     gene            complement(3440..>3697)
                     /locus_tag="AC159P_01095"
     CDS             complement(3440..>3697)
                     /locus_tag="AC159P_01095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694073.1"
                     /translation="DLGGRWLKQPRMHNAAFEFIGAGVELHPQHSALRELFADLLLKM
                     GRRDEAAESYRKAFALDPMIGKGATVEDYVAAKLNAAATKD"
BASE COUNT          608 a         1193 c         1291 g          605 t
ORIGIN      
        1 atccgcattc cgcaatcgtc agtcgccacc cacgccgacc tcttcccgct cgacttcgac
       61 cgtctcctcg ctctcgccgc gccgcggcgt gctcgtccgc tccggctcgt cggcctcggg
      121 ctgacgacgc ttgcgcgcga aggccgcgcg caaggccccg accgcgcggc gcgggatgag
      181 cgtgaaggag gcggcgcccc tgcgccacca ggcggtgttg ccgaggtcgt cgaagacgga
      241 gtaggcgacc ggcgtgacga gcagcgtcag cagcagcgag agcgtctggc cgccgatgac
      301 gacgacggcg atggagcgcc gctcttcggc gcccggcccc gtgccgagcg cgagcgggag
      361 catccccgcg acgagcgcga gcgtggtcat caggatgggc cgcaggcggt cgcggttggc
      421 ctggataatc gcctcgccgc gctcccagcc ttcgcgccgc aggttgttca tgtggtcgat
      481 ctggaggatg gagttcttct tgaccacgcc gaagagaacg aggatgccga gcgccgaata
      541 gaggttcagc gtgtcgccca agacccactg cgagaagagc gcgaagggca cggcgagcgg
      601 caggctcaag agaatcgtga ccgggtgaat cagactctcg aactgcgagg cgaggatcat
      661 gtacatgaag atgatcgaga gcatgaaggc ccagatgaac tccgtgaagg tgcgttccag
      721 ctcgcgcccg cggcccgaga tggccgtcgt gtaagcgggc ggcaggttca tcgagacggc
      781 ttcggcgcgc aacgcttcga gccggtcggc gagcgcgaag ccgggcgcca cggaggcgcg
      841 cacggagacc tgccgctggc ggtcgaggcg gtcgatgcgc gacggcgccg tctcgcgctt
      901 gatgttgacg aggttgtcga ggcggatgag gccgccgcgc gcagagggca cgtagaggtt
      961 gccgagcgtg cgcgggtcgt tgcggtcgcc ctcgttgacg cggagctgca cgtcgtaggc
     1021 ttcgttgacg gaggtgtcgt ggtagcgcgt cgcttccgtg tcgccgccga ccatgatgcg
     1081 cagggagttg gcgatgtccg cggtgttgac gccgagcgtg gcggcgcgct cgcggtcgat
     1141 ctcgatgcgc agctccggct tgttgagctt gagcgaagtg tccgcgtcca cgatgccgcc
     1201 gatcttctcc gtgcggtcgc gcaggtcttc ggcgtactcg acgagctgta tcaagtccgg
     1261 gccgcggaag atgaagtcga tgtcggtgcg cccgcccgcg ccgaagttga acgacggcgc
     1321 gttgcggacg ccgacgcgca gcggcgcgta cttgcgcatc cgccgtcgta tctcctgcat
     1381 cacgtcctgc tgcgcgtagt tgcccttgaa ggcgttcagt gggttgccgt tcagcgtttc
     1441 gcgccagatg cggctgatgg agacggtgcg ctcttcgtgc ggcgcgatgc ggacgtagat
     1501 gtcgccctgg ttgacgccgc cgaggaagcc gccgcccgcg ctcgccaaga ccatccgcac
     1561 gcccggcgtc ttcatcacct cctgctcgac ggcctcgacc gcctcgttca tcgccgtgat
     1621 gttcgtgccc tcgggcgcgt tcacgctgac ctcgaactcg gcctcgtcca cctgcgacgg
     1681 gatgaactcc tgccgcaccg cgcggtacaa aggcaccgac gagaagatga ccccgatggc
     1741 caggatcgag acggcgagcc ggtggcgcat cgagatggtg agcagccgcg tgtagacgcg
     1801 gtcgagccag gcgtagaagc ctttgcgcga ggcggcccct tcgccgtgac cgctaccgtg
     1861 gcccccgtgg ccgccgcccg acgccgcgtc ctcgacgcgg agcagccgcg aactcatcat
     1921 cggcgtcagc gtgaaggaga cgagcagcga gaccatgatc gccaccgccg ccgtgatgcc
     1981 gaactggtag aggaagcggc ccgagatcga ggacatgaac gagacgggca ggaagatgac
     2041 gacgagcgac agcgtcgtcg ccatgacggc gaggccgatg tccgcggtcg ccttgcgcgc
     2101 cgcctccatc ggtcgcatct tcttctcctc gatgaagcgg aagatgtttt ccagaacgac
     2161 gatggcgtcg tcgatcacca cgccgaccat cagcacgagc gcgagcatcg tcaccgagtt
     2221 gagcgtgaag tcgagcgcgc gcatcatgcc gaacgtggcg atgaccgagg tcgggatggc
     2281 gacgcccgcg atgacggtcg cgcgccacga gcgcatgaag aggagcacga ccagacacgc
     2341 gaggatgctg ccgaggacga ggtgcgtctc gatctcgtgg agcgcggcct cgatgtagcg
     2401 cgactggtcg cggatgatct ccatcttcac gtccgtcggc agttgcccca cgacgcgcgg
     2461 catcgcggcc ttgatgccgt tgatgacttc gatggtgttg gcgcccgact ggcggcgcac
     2521 ttgcagcgtc acggtcggga cgccattcag ccgcgcgacc gagcgctgct ccttcgtggt
     2581 gtcctcgaca cgtccaacgt cgcgcagccg cacggcggag ccgttgatgg tggccacgac
     2641 gaggtcgttg aaggcgcgcg ggtccgtgta gcgacccatc gtgcgcagca ccagctcctc
     2701 gcgccccgtg acgacgttgc cgccggggat gtccgcgttc tggcggtcga gcgcctgctg
     2761 cacggcagtg atcggaatct ggtaagccgc gagccggtcg gcgtccacga tgacgttgat
     2821 ggcgcgctcc aggccgccga cgacgctcac ctcgccgacg ccgcccacgc gctcgaactg
     2881 cgggcgcgcg agcttgtcgc ccagctccgt cagctcgcgc aggctccggt cggcggagag
     2941 cgagatcgtc atgatcggcg agctgtcgtt gttgaacttc tggacgacgg gcgggagcac
     3001 gtcttcgggg agactgcgga cgaccgtggc gacgcggtcg cgcacgtcct gcgcggcggt
     3061 ctcaaggtcg cggtcgagct tgaaggtggc gatgacgagc gacgtgcccg gccccgaaac
     3121 ggagcgcagc tcgtcgatgc cgtccaccgt gttgacgacc tcctcgatgg gctgcgagat
     3181 gagcgtctcg acctcttcgg cggaggcgcc gggcaggctc gtgcggacgc tcaccgtcgg
     3241 cagatcgacc gaaggaaacc ggtcaacgcc gaggcggaag aaactcgcgg cgccgacgac
     3301 gacgagcgac agcacgatca tcgccgcgaa cacggggcgc tggatacaga cttcggcgag
     3361 tttttgcata acttttattc aggagttcga agttcaaaat caggagcaag gttttcaact
     3421 tcgagcttcg aactccgaac taatctttcg ttgccgccgc gttcaacttc gcggcgacgt
     3481 aatcttcaac agtcgcgccc ttgccgatca tcggatcgag cgcgaaagcc ttacggtaac
     3541 tttcggcggc ctcgtcgcgc cggcccatct tcaaaagcag gtctgcgaac agctcgcgga
     3601 gcgcggagtg ctgcgggtgc agctcgacgc ccgcgccgat gaactcgaag gcggcgttgt
     3661 gcattctggg ctgcttcagc cagcgaccgc cgaggtc
//
LOCUS       DBNGOH010000055         2124 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4224050, whole genome shotgun sequence.
ACCESSION   DBNGOH010000055 DBNGOH010000000
VERSION     DBNGOH010000055.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2124)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2124)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2124
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4224050"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(104..1633)
                     /locus_tag="AC159P_01100"
     CDS             complement(104..1633)
                     /locus_tag="AC159P_01100"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HIR3694074.1"
                     /translation="MDIPQIVAGRFRIECELGRGGMGTVYRATHLGLERPVAIKVLKQ
                     EFAADPEVGERFMREARTMARLKHPRAAMIFDAGRLADGRPFIVMEYVEGATLAEVLS
                     REGTFAPARAVQVTAEICDVLSEAHALGIVHRDLKPSNIMLNERGVCVLDFGIAKVLA
                     TSTEVTRTHATTESGLVIGTPRYMSPEQCLGLPVGPASDLYSVGVLVYEMLAGRPPFV
                     DVHSSAVLVKQATSAPPPLLALKPELPRPLALAVHTLLAKNPAQRPAKAAEARALLER
                     SVVPPRAVFYEGDLAPLASTISHLDAGRFNFIRMATACAIIAMLGVVIFAWAGSEGGR
                     NRERESLFGAGSLTERARLSSRSAAAGFAAAAPPAPLTPESARRTAETLARGAVGDAL
                     ILRTKGGQTIAAVREEREQGTTHLLVIERKGGRTFRITARAPLDAEGFRGANWAAEAV
                     DLDGDGYDEVLCTGTSARGDLNGNRRVVLYVPRTRQTYSLKAPPDARDPNTARARWQK
                     E"
     gene            complement(1869..>2124)
                     /locus_tag="AC159P_01105"
     CDS             complement(1869..>2124)
                     /locus_tag="AC159P_01105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694075.1"
                     /translation="CAEAPRTQDSKSAPPARASNMNTSSGPAGSQNAAGAEPLQGSRF
                     GTRLTNQSVGAGINDNTDTRGSNKAAGGAKKSNPAGRKKR"
BASE COUNT          286 a          674 c          804 g          360 t
ORIGIN      
        1 tcctgaaatc aatgataaga ggcgcgctgc ctcccgcctc tgccttgatg acaggcacag
       61 tgcttccgat ccacacttct gcctactgcc ttctgcctac cgcctactct ttctgccagc
      121 gagcgcgggc ggtgttgggg tcgcgcgcgt cgggcggtgc cttgaggctg taggtctggc
      181 gtgtgcgcgg gacgtagagg acgacgcggc ggttgccgtt gaggtcgccg cgggccgagg
      241 tgcctgtgca gagcacttcg tcgtagccgt cgccgtcgag atcgacggct tcggcggccc
      301 agttggcgcc gcggaagcct tcggcgtcga gcggcgcgcg cgccgtgatg cggaaagtcc
      361 gtccgccctt gcgctcgatg acgagcaggt gcgtggtgcc ctgctcgcgc tcctcgcgca
      421 cggcggcgat ggtctggccg cccttcgtgc ggaggatgag cgcgtcgccg accgcgccgc
      481 gcgccagcgt ctcggcggtg cggcgggccg actcgggcgt gagcggcgcg ggcggcgccg
      541 ccgcggcgaa gcccgccgcc gccgagcgcg aagagaggcg cgcgcgctcc gtcaaagacc
      601 ccgcgccgaa gaggctttcg cgctcgcggt tgcgcccccc ctcgctgccg gcccacgcga
      661 agatgacgac gccgagcatg gcgatgatgg cgcacgcggt cgccatgcgg atgaagttga
      721 agcggcccgc gtcgaggtgg ctgatggtgg aggcgagcgg cgccagatcg ccttcgtaga
      781 agacggcgcg gggcgggacg acgctgcgtt cgagcagcgc gcgcgcctcc gccgccttgg
      841 cgggccgctg cgcggggttc ttggcgagca gcgtgtggac ggcgagcgcg agcgggcgcg
      901 gcagctcggg cttgagggcg agcagcggcg gcggcgccga ggtcgcctgc ttgacgagca
      961 ccgcggacga gtgcacgtcc acgaaggggg ggcgcccggc gagcatctcg tagacgagca
     1021 cgccgacgct gtaaagatcg gaggccgggc cgaccggcag gccgaggcat tgctcgggcg
     1081 acatgtagcg cggcgtgccg atgacgaggc cggactcggt cgtggcgtgc gtgcgcgtga
     1141 cctcggtcga ggtggcaagg actttcgcga tgccgaagtc gagcacgcag acgccgcgct
     1201 cgttgagcat gatgttcgag ggcttgaggt cgcggtggac gatgccgagc gcgtgcgcct
     1261 cgctgagtac gtcgcagatt tcggcggtca cttgcacggc gcgcgcgggc gcgaacgtgc
     1321 cttcgcgcga gagcacctcg gcgagcgtgg cgccctcgac gtactccatg acgatgaagg
     1381 ggcgcccgtc ggcgagtctt ccggcgtcga agatcatcgc ggcgcgcggg tgcttgaggc
     1441 gggccatcgt gcgggcctcg cgcatgaagc gctcgccgac ctctgggtcg gcggcgaact
     1501 cctgcttgag gactttgatg gcgacgggcc gttcgagacc gaggtgtgtg gcgcggtaga
     1561 ccgtgcccat gccgcccctg ccgagttcgc actcgatgcg gaagcgtccg gcgacgatct
     1621 gggggatgtc cataacgaga ccctctcttc tcttgagtta agggtcgctc agggcggtgc
     1681 gctcttttct cgttctatgc ggggcgcaga gaataagggg cttcaaccga gcaacacggg
     1741 tgaaacttat tcaggttgtc gtttgagttg gggagacaaa tcgtccggca cgctctcggg
     1801 gagcggcgcc ggacgaagga gagtgtagca cttttgccga caggcgcaag ctttccggcg
     1861 cccgcatctc agcgtttttt cctgcccgcg ggattcgact ttttcgcccc gcccgcggcc
     1921 ttgttgctgc cgcgcgtgtc ggtgttgtcg ttgatgccgg ccccgaccga ttggttcgtg
     1981 agccgcgtgc cgaaccgcga accctgcaaa ggctccgcac ccgcggcgtt ctggctgccc
     2041 gccgggccgg acgacgtgtt catgttggaa gcacgggcgg ggggagcgct cttgctgtcc
     2101 tgcgtcctcg gggcttcggc gcac
//
LOCUS       DBNGOH010000056         2157 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3124584, whole genome shotgun sequence.
ACCESSION   DBNGOH010000056 DBNGOH010000000
VERSION     DBNGOH010000056.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2157)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2157)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2157
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3124584"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            71..1066
                     /locus_tag="AC159P_01110"
     CDS             71..1066
                     /locus_tag="AC159P_01110"
                     /inference="COORDINATES: protein motif:HMM:NF013986.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HIR3694076.1"
                     /translation="MKVRRAPVKFRASAKHSNLRARGLVVGVDGGATKTRAVILNGRQ
                     HVLGEGTAGPSNPLRVGVRDATVAVREAVERACAAARVRRVDLVAAEVGLAGVRREDL
                     RAAMRAALAELGIKSLQVVTDADIALYGATEGKPGLVIIAGTGSVCCGRNARGVHVCA
                     GGWGPLAGDEGSGSWIARRGLQAVAHATDGRGDQTPLVAAACDYFNVASPDDLSTALY
                     APNMTNDRIAGFGKSVIEAAKGGDRVAAAIVVDAGRELGRAAAAVIRRLRMGRERFQV
                     GYVGGVFAAGDLMLDSLQEEVARAAPKAYLAPPLLAPAEAAARMAREQVQLALAG"
     gene            1238..>2157
                     /gene="murQ"
                     /locus_tag="AC159P_01115"
     CDS             1238..>2157
                     /gene="murQ"
                     /locus_tag="AC159P_01115"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010498781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HIR3694077.1"
                     /translation="MDNPEQIPVTEQENPRTREIGRLPTVEVVRLMNEEDARVAEAVG
                     RALPEVARAVDGIVARLEHGGRLFHVGTGTSGRLGVLDASECPPTFGVAPELVQGVIA
                     GGYDALHRAVEASEDDREAGGRDLDARGLTDKDAVVGIAASGRTPYTVGAVEHARSLG
                     AFTAAVACAPGSELTRAAEVAIVAVVGPEVITGSTRLKAGTAQKLILNMISTAAMIRL
                     GYVTGNRMTNLLPRNVKLRARAERIFAAETGADEDASRAAIEAAGGDLRLALVMHHTG
                     ASRVEAALALEASRGVVERAVRILQGGNGA"
BASE COUNT          330 a          724 c          793 g          310 t
ORIGIN      
        1 attgaacctc ttttttcgac cacgcgaggc ggcgccccga aacgcgaggg gcgcgcgccg
       61 gagtcccccg atgaaggtca ggcgcgcgcc agtcaaattc cgagcatccg ccaaacattc
      121 caacctgcga gcgcgcggcc tcgtcgtcgg cgttgacggc ggggccacga agacgcgcgc
      181 cgtcatcctc aacggcaggc agcacgtcct cggcgaaggg acggcggggc cttcgaaccc
      241 gctgcgcgtg ggcgtgcgcg acgccacggt ggcggtgcgc gaggcggtcg agcgcgcgtg
      301 cgccgccgcg cgcgtgcgcc gcgtggacct cgtcgccgcg gaggtgggtc tggcgggcgt
      361 gcggcgcgaa gacctgcgcg cggcgatgcg ggcggcgctg gcggaactcg gcatcaagtc
      421 attgcaggtg gtgacggacg cggacatcgc gctctacggc gcgaccgagg gcaagcccgg
      481 tctcgtcatc atcgccggga cggggtcggt ctgctgcggg cggaacgcgc gcggcgtaca
      541 cgtctgcgcg ggcgggtggg ggcctttggc gggcgacgag ggcagcggct cttggatcgc
      601 gcggcggggc ttgcaggccg tggcgcacgc gacggacggg cgcggcgacc agacgccgct
      661 cgtcgcggcg gcgtgcgact acttcaacgt cgcctcgccg gacgacctct cgacggcgct
      721 ctacgcgccg aacatgacga acgaccgcat cgcgggcttc ggcaagtcgg tcatcgaggc
      781 ggccaagggc ggcgaccgcg tcgccgccgc aatcgtcgtc gacgcgggca gggaactcgg
      841 gcgcgccgcc gccgccgtca tccgcaggct ccgcatgggg cgcgagcgtt ttcaggtggg
      901 ctacgtcggc ggcgtcttcg ccgcgggcga cctgatgctc gattcgctgc aagaggaggt
      961 cgcgcgcgcc gcgccgaaag cgtacctcgc gccgccgctg ctcgcgcccg ccgaggccgc
     1021 cgcgcgcatg gcccgcgagc aggtgcagct cgcgctcgcg ggctgaaaca gaaatgatga
     1081 atgcgggttg atgaatgatg aatgacaagc acgctgcccc ttatcattca tcaatcatga
     1141 agggcagcgt gccattcatg attgatgaac ggagcgctgc ctctcatcat tcaagtttct
     1201 tgacagtcat cattcatcaa cccgcattca tcatttaatg gataaccccg aacagattcc
     1261 cgtgactgaa caggagaacc cgcggacgcg ggagatcggt aggctgccga ccgtggaggt
     1321 cgtgcgcttg atgaacgagg aggacgcgcg cgtggccgag gcagtcgggc gcgcgctgcc
     1381 ggaagtggcg cgcgcggtcg atggcatcgt cgcccgtctt gaacacggcg ggcgactttt
     1441 ccatgtcggg acgggcactt cgggtaggct cggcgtgctc gacgcgtcgg agtgcccgcc
     1501 gaccttcggc gtcgcgccgg agttggtaca gggcgtgatc gcgggcgggt acgacgcgct
     1561 gcatcgcgcg gtcgaagcat ccgaagacga ccgcgaggcg ggcgggcgcg acctcgacgc
     1621 gcgcggcttg acggacaaag acgcggtggt cggcatcgcc gcctccggaa ggacgccgta
     1681 caccgtcggc gccgtcgagc acgcgcgctc tctcggtgcg ttcaccgccg ccgtggcgtg
     1741 cgcgcccggc tcggagttga cgcgcgcggc tgaggtcgcc atcgtcgcgg tggtcggccc
     1801 cgaggtaatc accggctcga cgcgtttgaa ggcgggcacc gcgcagaagt tgatcctcaa
     1861 catgatctcg accgccgcga tgatccgcct cggctacgtc accggcaacc ggatgacgaa
     1921 cctgctgccg cgcaacgtca agctccgcgc gcgcgccgag cgcatcttcg ccgccgaaac
     1981 cggcgccgac gaagacgcct cgcgcgcggc catcgaagcg gcgggcggcg acctccggct
     2041 cgcgctcgtc atgcaccaca cgggagcctc gcgcgtagag gcggcgctcg ccctcgaagc
     2101 ctcgcgcggc gtcgtcgaac gcgcggtcag gattcttcag ggcggtaacg gcgcctg
//
LOCUS       DBNGOH010000057         4362 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_8627, whole genome shotgun sequence.
ACCESSION   DBNGOH010000057 DBNGOH010000000
VERSION     DBNGOH010000057.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4362)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4362)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4362
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_8627"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2048)
                     /locus_tag="AC159P_01120"
     CDS             complement(<1..2048)
                     /locus_tag="AC159P_01120"
                     /inference="COORDINATES: protein motif:HMM:NF051698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR3694078.1"
                     /translation="MKYRWPSVVALLALMLAWASPSFAQTDRGGIIGRVTDPGGAVIT
                     KAKVTATNVENNEVRETATTDEGNYTIPQLQAATYRLTVEAPGFKTAAFEDVKVAVSI
                     TRTVDVTLEVGEVGDTVTVSAESVPILQTEAPVQQLNVTERQVRELPLLVAAESGGRS
                     PLSFIFLDSSVVSNDTPGGTGTTSGSTGTNATNFRINGGQGLGADILIDGAQTRRGEN
                     GTFFSEVAPGPNAFQEFTVSTSSYSAEFGNSTGGVINFTIKTGSNDFHGEAYLFHINN
                     ALNANIDRNRILNQNNANCVLREVGCLERPLDRQFDFGFSVSGPVTLPHFGEGGPTYH
                     SFKDKTFFFFNYGGYRTFQSESVDITVPTQRMRQGDFGELLTDPYVLQFFGGPVQIFD
                     PNQPAGARTAIPGNRLDLYEGGSRISAIGRNFVNLFPLPNQTGPNGSTVFRNFRASTT
                     QTSRTDYYVTKITHNVTDRQTVNFSYTFRKLPSVKGGFPRFPEPFVQQGVWDQLFKSY
                     YARLQHDWTLTPSFINHFNAGFSRSDVSNNNFTDGIPASTLGLRPNATQDRGLPLVGF
                     PGYGDPVNSTDPRAYQAGGSTFFDNRVRDNSVHLSDVATLIRGRHTMKFGGELRLQQL
                     NNAAHFDIGGNFNFRSNQTGNTNDFTQGWPIASLITGRPEFSFNSNQTIDPALRYMSY
                     A"
     gene            2460..3476
                     /locus_tag="AC159P_01125"
     CDS             2460..3476
                     /locus_tag="AC159P_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020714040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinan endo-1,5-alpha-L-arabinosidase"
                     /protein_id="HIR3694079.1"
                     /translation="MKALRHLNIAVAALLLLPCGPRFFAQGANAQGPRELQGDIRHVH
                     DPTVIKEGDTFYLFSTRAGISVRCSTDLVRWRLCGDVFGHLPQWAVEDVPGLRGLWAP
                     DVSYFNGRYHLYYSASTFGKNRSSIGLATNRTLDSSSERYRWEDQGKVIGSSAADDWN
                     AIDPNIVLDEQGQPWLSFGSFWGGIKLRRIDPATGKLSAADETLYSLASRPRSKELPG
                     AVEAPVIHRKNGYYYLFVSFDFCCRGEKSTYHIRVGRSRRVTGPYTDRSGKPMNEGGG
                     TLVLEGAGRWRGPGHCAVLQDKDGEKLVYHAYDAEWRGVPTLRIHPLHWDAEGWPTVS
                     PPDQ"
     gene            complement(3656..>4362)
                     /locus_tag="AC159P_01130"
     CDS             complement(3656..>4362)
                     /locus_tag="AC159P_01130"
                     /inference="COORDINATES: protein motif:HMM:NF024299.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3694080.1"
                     /translation="SLAHLVHARRVAPEAPGVLYDFGVTTLQMGLLLDALPAFEQLHR
                     LRPREPAYVYGLAAARFRKGELTEAVRLLNDYVAARPRDPAGSYLLGAALQRLGQFAA
                     ARAALERSLALKPDPDVEYLLGVTLEQEGNRAAAIAVLRRVVVARPEHAAAHAALGAA
                     FREQGDYAGARAELERAVELDPGDLRASYQLGLVYAKLGDKEAAQKMFARADELRGKE
                     RRRETVILKLIEPPEN"
BASE COUNT          713 a         1370 c         1493 g          786 t
ORIGIN      
        1 gcgtaggaca tgtagcgcag cgccgggtcg atcgtctggt tcgagttgaa cgagaactcg
       61 ggccgccccg tgatgaggct ggcgatgggc cagccctgtg tgaagtcgtt cgtgttgccg
      121 gtctggttgc tgcggaagtt gaagttgccg ccgatgtcga agtgggccgc gttgttgagc
      181 tgctggaggc ggagttcccc gccgaacttc atcgtgtggc gcccgcggat gagggtcgcc
      241 acgtcggaga ggtgaaccga gttgtcgcgc acgcggttgt cgaagaaggt ggagccgccg
      301 gcctggtagg cgcgcgggtc ggtcgagttc acagggtcgc cgtagccggg gaacccgacg
      361 agcggcaggc cccgatcctg tgtcgcgttc gggcgcaggc cgagcgtgct ggccgggatg
      421 ccgtccgtga agttgttgtt cgaaacgtcg gagcgcgaga agcccgcgtt gaagtggttg
      481 atgaacgacg gcgtcagcgt ccagtcgtgt tgcaggcgcg cgtagtagga cttgaacaac
      541 tggtcccaca cgccctgctg gacgaacggc tcggggaagc gcgggaagcc gcccttgacg
      601 ctcggcagct tacggaacgt gtagctgaag ttgaccgtct gccggtcggt cacgttgtgc
      661 gtgatcttcg tcacgtagta gtcggtgcgc gaggtctgcg tcgtcgaggc gcggaagttg
      721 cggaacacgg tcgagccgtt cggcccggtc tggttcggca gcgggaagag gttgacgaag
      781 ttgcgcccga tggcggagat gcgcgagccg ccctcgtaga ggtcgagccg gttgccgggg
      841 atggccgtgc gggcgcccgc cggctgattc gggtcgaaaa tctggacggg gccgccgaag
      901 aactggagca cgtaggggtc ggtcagcagc tcgccgaagt cgccctgccg catcctctgc
      961 gtcggcacgg tgatgtccac cgactcggac tggaacgtgc ggtagccgcc gtagttgaag
     1021 aagaagaagg tcttgtcctt gaagctgtgg taggtcgggc cgccctcgcc gaagtggggc
     1081 agggtgacgg ggccgctgac cgagaagccg aaatcgaact ggcggtcgag cggcctttcg
     1141 agacagccga cttcgcggag cacgcagtta gcgttgttct ggttgagaat ccggttgcgg
     1201 tcgatgttgg cgttcagcgc gttgttgatg tggaagaggt aggcttcgcc gtggaagtcg
     1261 ttgctgcccg tcttgatcgt gaagttgatg accccgcccg tcgagttgcc gaactcagcc
     1321 gagtagctcg aagtcgagac ggtgaactcc tgaaaggcgt tcgggccggg cgccacctcg
     1381 gagaagaagg tgccgttctc gccgcgccgc gtctgcgccc cgtcgatgag gatgtcggcg
     1441 ccgagcccct gcccgccgtt gatgcggaag ttggtcgcgt tcgtgcccgt cgagccggag
     1501 gtcgtgcccg tcccccccgg cgtgtcgttg gagaccacgc tgctgtcgag gaagatgaac
     1561 gagagcggcg agcgcccgcc cgattcggcg gcgacgagca gcggcagttc tctcacctgc
     1621 cgctccgtca cgttgagctg ctggacgggc gcctcggtct gcaagatcgg cacgctctcc
     1681 gccgagaccg tcaccgtgtc gccgacctcg ccgacttcga gcgtcacgtc cacggtgcgc
     1741 gtgatcgaga cggcgacctt cacgtcctcg aaggccgcgg tcttgaagcc gggcgcctcg
     1801 accgtcagcc ggtaggtggc cgcctggagc tgcgggatcg tgtagttgcc ttcgtcggtc
     1861 gtcgcggtct cgcgcacctc gttgttctcg acgttcgtgg ccgtcacctt cgccttcgtg
     1921 atgacggcgc cgccggggtc ggtcaccctg ccgatgatcc cgccgcggtc ggtctgcgcg
     1981 aaactgggtg aagcccacgc gagcatgagc gcgagcagtg cgacgacact gggccaacgg
     2041 tatttcatgg tctccctccg ggttcagacg aatcgcaaag gtcgccgtgt ggaggggggc
     2101 ggaactatcg cccccgcacg gacgaaggtt gtcgagggta aactcctccc cccaacgtaa
     2161 gcgcctgcaa ctgacttcct gtcaatcgaa gcgaacggtg aaaggatcgc ttgctgatta
     2221 ttgccgttga aattgaatgg tggaataagg gcgcgggcgt cagtcggtca acctaaaggt
     2281 ggacaccccg ctaaaacgac agtatgaata ttttttgatc gctcgcgcct tttgttcgtt
     2341 tacaccacaa aagtgagggt gtcaagaatt atgttcgttc ttttacgaat agacgcactt
     2401 tttgcgcccg cgcgcccgtg ctcgtgatag gcttggcccc tccgaacaga cctatgtgca
     2461 tgaaggcgct ccgtcatctc aacatcgccg tcgcggcctt gctcctcctg ccgtgcggcc
     2521 cgcgcttctt cgcgcagggc gcgaacgcac aggggccgcg cgagttgcag ggagacatcc
     2581 gccacgtcca cgacccgacc gtgatcaaag agggcgacac cttctacctt ttttccacgc
     2641 gggccggcat cagcgtccgc tgctcgacag acctcgtgcg ctggcgtctg tgcggcgacg
     2701 tgttcgggca cctgccgcag tgggcggtcg aggacgtgcc ggggctgcgc ggcctgtggg
     2761 cgccggacgt gtcctacttc aatggcaggt atcacctcta ttactccgcg tccaccttcg
     2821 gcaagaaccg ttcgagcatc gggctggcga ccaaccgcac gctcgattct tcgagcgaac
     2881 gctataggtg ggaggatcag gggaaagtca tcggctcgtc cgccgcggac gactggaacg
     2941 ccatcgaccc caacatcgtc ctcgacgagc aagggcagcc gtggctctct ttcgggagtt
     3001 tctggggcgg catcaagctg cgccggatag accccgcgac gggcaaactt tccgcggcgg
     3061 acgagacgct gtactcgctc gccagccggc cccgttcgaa agagctgccg ggcgccgtcg
     3121 aggcgccggt catccacagg aagaacggct actactacct cttcgtctcc ttcgacttct
     3181 gctgccgcgg cgagaagagc acttaccaca tccgcgtcgg gcgctcgcgc cgcgtcacag
     3241 gcccctacac ggatcggagc ggcaagccga tgaacgaggg cgggggcacc ctcgtcctcg
     3301 aaggcgcggg ccgctggcgc gggccgggcc actgcgccgt gctgcaagac aaggacggcg
     3361 agaagctcgt ctaccacgcc tacgacgccg agtggcgcgg cgtgccgacc ttacgcatcc
     3421 accctctcca ctgggacgcc gaaggttggc cgaccgtctc gccgcccgac caatagagca
     3481 agtttcagtt gcttgtagaa cgcgcgcggt aactgttggg gcagggcttg tccctgcccg
     3541 ccgctgacgc atctattcaa gattgaatac tgtgcacaaa gggcagggac aagccctacc
     3601 cctacaatct tcacgcacga ctcacatcca ttcgcaggcc gcggtgacgt gagagttaat
     3661 tttccggcgg ctcgatcaac ttcaggatca cggtctcgcg ccggcgctcc ttgccgcgca
     3721 gctcgtcggc gcgcgcgaac attttctgcg ccgcctcctt gtcgccgagc ttcgcgtaaa
     3781 cgagtccgag ttgatagctg gcgcgcaggt ctccggggtc gagttcgacc gcgcgctcaa
     3841 gctcggcgcg cgcgcccgcg tagtcgccct gttcgcggaa agccgcgccg agcgctgcgt
     3901 gcgccgccgc gtgctcgggg cgcgcgacga cgacgcgccg cagcacggcg atggccgccg
     3961 cgcgattccc ttcctgttcc agcgtcacgc cgagcaggta ctcaacgtcg gggtccggtt
     4021 tgagagcgag actgcgttcg agcgcggcgc gcgccgccgc gaactggccg aggcgttgga
     4081 gcgccgcgcc gagcaggtag gagcctgcgg ggtcgcgcgg gcgcgccgcg acgtaatcat
     4141 tcaacagacg cacggcctcc gtcagctcgc ccttcctgaa ccgcgccgcc gccagcccgt
     4201 agacgtacgc cggttcgcgt ggccgcagcc ggtgcaactg ctcgaaggcc ggcagagcgt
     4261 cgagcagcag acccatctgg agcgtcgtca cgccgaagtc gtacaacacg cccggcgctt
     4321 cgggcgcgac gcggcgcgcg tggacgagat gcgcgagcga ct
//
LOCUS       DBNGOH010000058         3876 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3308514, whole genome shotgun sequence.
ACCESSION   DBNGOH010000058 DBNGOH010000000
VERSION     DBNGOH010000058.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3876)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3876)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3876
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3308514"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(67..3042)
                     /locus_tag="AC159P_01135"
     CDS             complement(67..3042)
                     /locus_tag="AC159P_01135"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3694081.1"
                     /translation="MRLSNHPYKFGVLLVALLCALMIFGGAGARALDPRREPAQFTRQ
                     AWQTENGLPQNTVHAVAQTEDGYLWVATEEGLARFDGQGFAVFDIQNTPQLKSNDIRH
                     LLPGFENVFWVSTAVGLVRLSHGEWTTYTTREGLPGDDVEVAYEAREGGLWVGTGDGL
                     ARYRDGAFTSYTTRDGLVSNSVQSLCEDAAGALWVGTSDGLSRFADGKFTNYAAHDGL
                     AFSSVEAIVRGEDGRLWFGTLNGLVSYDGGQFKSYTTADGLPNNRVLSLHADGAGALW
                     VGTAGGLSRMREGRFENYAPGDALSNGIVLSIFEDAEGSLWVGTESGGLHQLKDKKFT
                     TYTAKDGLAGDLVKAVYEDRRGQVWVGTYGSGLSLLARDGKLTTYTTKEGLASNIILA
                     LFDDAEGSLWVGTPDGLSKYRDGRFTTYTSADGLANDFVRSIYADRAGALWVGTRGGL
                     TRMKDGAFTIYTTRDGLPDDFVGTIYEDAEGSIWAGTLGGLSRFKDGAFTSYTTRDGL
                     SDNVVIALHGDAEGRLWIGTNGGGLNLLREGRFTSYTTRDGLPHDTVYRILEDGAGNL
                     WMSCNKGVFRVSKAELEDFAGGRTKTFSPVVYGTADGMATRECSGGGHPSGWRDRQGR
                     LWFSTIKGVAVIDPAGARTNAQPPPAVVEQVRVDGELVNMFAKTELAPGKTRFDFHYA
                     GLSFVAPEKVRYKYRLTGFDKDWVDGGARRVAYYTNLGPGDYRFEVLACNNDGVWSRA
                     PASYAFTLKPHFYRTPWFYALVALGLAFVAAQVYLLRVRQMKARFAAVLAERNRIARE
                     IHDNLAQEILGVSVQLELVARLMPRSTDAAKTHLDRARALVRSSIAEARRYVWDLRSQ
                     SLDDRDLATALSEMTRRLAAESGVQTQFQVSGTFRPLTPQVENNLLRIGQEAVNNAVR
                     HARAQLITVNLSFDDARVRLSVRDDGRGFDPRNGSGGDGHFGIVGMRERAAEMGGTLN
                     VESRAGEGTEVLVGVPIEG"
     gene            3242..3775
                     /locus_tag="AC159P_01140"
     CDS             3242..3775
                     /locus_tag="AC159P_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694082.1"
                     /translation="MQTSKMLRAFVAAALVLAACAGAAAGTRARWRGATAQELKGVIP
                     ARAPVNKENIETELRTASGVTDGRGRFIAGVVMITAGYSAEGKYSHYLLTQVPLRVAD
                     FQLAPGQYVFGYRRTGDETIRVSFYRAATGEAVGETDAHRNRKSSMVRSLLINPPVGG
                     KANMQVGRFIFEYRVND"
BASE COUNT          587 a         1299 c         1342 g          648 t
ORIGIN      
        1 ggtcgtcgat cacgatgatg cggattttgt cgttgctcac ggtcgttaag agtatgcgaa
       61 agcgcttcaa ccttcaatgg gcacgccgac cagaacttcg gtgccctcgc ctgcgcggct
      121 ttcgacattg agggtgccgc ccatctcggc ggcgcgctcg cgcatgccga cgatgccgaa
      181 gtggccgtcg ccgccggagc cgttgcgcgg gtcgaagccg cgcccgtcgt cgcggacgct
      241 caggcgcacc cgcgcgtcgt cgaaagaaag gttcacggtg atgagctgcg cgcgcgcgtg
      301 gcgcacggcg ttgttgacgg cctcctgccc gatgcgcagg aggttgttct cgacctgcgg
      361 cgtgagcggg cggaaggtgc cgctgacctg gaactgcgtc tgcacgccgc tctcggcggc
      421 gaggcggcgc gtcatctccg agagcgcggt ggcgaggtcg cggtcgtcga gcgactgcga
      481 gcgcaagtcc cagacgtagc gccgcgcctc ggcgatgctg ctgcgcacca gcgcgcgggc
      541 gcggtcgagg tgcgtcttcg ccgcatcggt cgagcgcggc atcaggcggg cgaccagttc
      601 gagctggacg gagacgccga ggatttcctg cgcgaggttg tcgtgaatct cgcgcgcgat
      661 gcggttgcgc tccgcgagca ccgccgcgaa gcgcgccttc atctgccgca cgcgcagcag
      721 gtagacctgc gcggcgacga aggcgaggcc gagggcgacg agcgcgtaga accagggcgt
      781 gcggtagaag tgcggcttca gcgtgaaggc gtacgaggcg ggcgcgcggc tccacacgcc
      841 gtcgttgttg caggccagca cctcgaagcg gtagtcgccc ggcccgaggt tcgtgtagta
      901 ggcgacgcgg cgcgcgcccc cgtccaccca gtctttgtcg aaccccgtga gccggtactt
      961 gtagcgcacc ttctcgggcg cgacgaagct gaggcccgcg tagtggaagt cgaagcgcgt
     1021 cttgcccggc gcgagttcgg tcttggcgaa catgttgacc agctcgccgt caacgcgcac
     1081 ctgctcgacg acggcgggcg gcggctgcgc gttcgtgcgc gcgcccgccg ggtcgatcac
     1141 cgcgacgccc ttgatggtcg agaaccagag ccgcccctgt cggtcgcgcc agcccgaagg
     1201 gtggccgccg ccgctgcact cgcgcgtcgc catcccgtcc gccgtcccgt agacgaccgg
     1261 gctgaaagtc ttcgtcctcc cgccggcaaa gtcttcaagc tccgccttcg agacgcggaa
     1321 gacgcccttg ttgcagctca tccacaaatt gcccgcgccg tcttcgagga tgcggtagac
     1381 ggtgtcgtgc ggcaggccgt cgcgcgtcgt gtaggaggtg aacctgcctt cgcgcagaag
     1441 gttgaggccg ccgccgttgg tgccgatcca gagccgcccc tctgcgtcgc cgtggagcgc
     1501 gatgacgacg ttgtcggaga ggccgtcgcg cgtcgtgtaa gaggtgaacg cgccgtcctt
     1561 gaatctgctg aggccgccga gcgtcccggc ccagatgcta ccttcggcgt cttcgtagat
     1621 cgtcccgacg aagtcgtcgg gcagcccgtc gcgcgtcgtg tagatcgtga aggcgccgtc
     1681 cttcatcctc gtcaggccgc cgcgcgtgcc gacccagagc gcgcccgcgc ggtcggcgta
     1741 gatcgagcgc acgaagtcgt tcgccaaccc gtccgcggag gtgtaggtcg tgaacctgcc
     1801 gtccctgtat ttactgaggc cgtcgggcgt cccgacccag aggctgcctt cggcgtcgtc
     1861 gaagagcgcg aggatgatgt tgctggcaag gccctccttc gtcgtgtagg tcgtgagctt
     1921 gccgtcgcgt gcgagcaggc tgaggccgct gccgtaggtg ccgacccaga cctgcccgcg
     1981 gcggtcttcg tagaccgcct tgacgaggtc gccggccaat ccatccttcg ccgtgtaggt
     2041 cgtgaacttt ttgtccttca gttggtgcag tccgcccgac tcggtgccga cccagaggct
     2101 gccctctgcg tcctcgaaga tcgacaggac gatgccgttc gagagcgcgt cgcccggcgc
     2161 gtaattctcg aagcgacctt cgcgcatgcg gctgaggccg cccgccgtgc cgacccacaa
     2221 cgcgcccgcg ccgtccgcgt gcagagagag gacgcggttg ttcggcagcc cgtccgcggt
     2281 cgtgtagctc ttgaactgtc cgccgtcgta gctgaccaac ccgttgagcg tgccgaacca
     2341 gaggcgaccg tcctcgcccc gcacgatggc ctccacgctg ctgaaggcga ggccgtcgtg
     2401 cgcggcgtag ttcgtgaact tgccgtccgc gaatcgactg aggccgtcgc tcgtgccgac
     2461 ccagagcgcg cccgccgcgt cctcgcagag tgattggacg ctgttgctga cgaggccgtc
     2521 gcgcgtggtg taggaagtga acgcgccgtc gcggtaacgg gcgaggccgt cgcccgtgcc
     2581 gacccacagg ccgccctcgc gcgcctcgta ggccacctcc acgtcgtcgc cgggcagccc
     2641 ctcgcgcgtg gtgtaggtcg tccactcgcc gtgggagagg cggacgaggc ccaccgcggt
     2701 gctgacccag aaaacgtttt caaagccggg cagcaggtgg cggatgtcgt tgctcttgag
     2761 ctgcggcgtg ttctgtatgt cgaagacggc gaatccctgg ccgtcgaagc gcgcgagtcc
     2821 ctcctcggtg gcgacccaga ggtagccgtc ctcggtctgc gcgaccgcgt ggacggtgtt
     2881 ctgcggcagg ccgttctcgg tctgccacgc ctgccgtgtg aactgcgccg gctcgcggcg
     2941 cgggtcgagc gcgcgcgccc ccgcgccgcc gaagatcatc agcgcacaga gcagcgcgac
     3001 gaggagcaca ccgaatttgt aagggtggtt tgacaggcgc atcggttaga ctgcactcga
     3061 cgtggccggc gcgttaagaa gctgcggcga attataacgg aagacggacg gacaggtaag
     3121 cgcgccgccc gcccgcaagc gcggccccgc ccggtgacgg cggcgcggga tgcgtttatg
     3181 atgttgacgc ccgctgagcc gccgttctga tttcgtcggc gcgcgggggc cggaggggaa
     3241 gatgcagacc tcgaaaatgt tgagagcctt cgtggcggcg gcgctcgtgc tcgcggcgtg
     3301 cgcgggcgcg gctgccggaa cccgcgcgcg ctggcgcggc gccacggcgc aggagttgaa
     3361 gggcgtcatc ccggcgcgcg cgcccgtcaa caaggagaac atcgagacgg agctgcgcac
     3421 cgcctcgggc gtcaccgacg gtcgcggcag gttcatcgcg ggcgtggtga tgattaccgc
     3481 cggctactcc gccgagggca agtactcgca ctacctgctc acgcaggtgc cgctccgcgt
     3541 cgccgacttc cagctcgcgc ccggccagta cgtcttcggc taccggcgca cgggcgacga
     3601 gaccatccgc gtcagcttct accgcgccgc caccggcgag gccgtcggcg agacggacgc
     3661 ccaccgcaac cgcaagagca gcatggtgcg ctcgctcctc atcaaccctc ccgtcggcgg
     3721 caaagccaac atgcaggtcg ggcgctttat cttcgaatac agagtgaacg attaaagaaa
     3781 gtcagaagtc agaacgcaga acgatgaatg aaagacaaaa tgcttttcat tcatcgttct
     3841 gcgttctgac ttctgacttc tcttccccgt ctccac
//
LOCUS       DBNGOH010000059         5803 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3125075, whole genome shotgun sequence.
ACCESSION   DBNGOH010000059 DBNGOH010000000
VERSION     DBNGOH010000059.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5803)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5803)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5803
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3125075"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..277
                     /locus_tag="AC159P_01145"
     CDS             <1..277
                     /locus_tag="AC159P_01145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694083.1"
                     /translation="PSLGADPGEFAFGLRSRWLDASGERTVSIVGDIPHLAYDLAPGE
                     TVPVPLTLAPPAAPGDYLLEVHMLHEGVGLFLDRGSQPLRLAVRVAR"
     gene            346..1281
                     /locus_tag="AC159P_01150"
     CDS             346..1281
                     /locus_tag="AC159P_01150"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR3694084.1"
                     /translation="MYKNRRIAVVVPAYNEERHVGEVLRGVPDYVDHVIAVDDCSADG
                     TRAAIESCDDARVVALCTPANLGVGGATLLGYRKALELGCDVAVKMDGDGQMPPAHLP
                     LLLDAVVEHGYDYAKGNRFLAGRSLAEMPKVRLFGNVVLTFVNKLASGYWHIFDPQNG
                     YTAVTAEALRRLDFGSLHRRYFFENDMLIALNFHNARVRDVAIPARYGDERSDLNPFK
                     VGVTFPLLFIPRFWRRIYRKYVLHDFSPVALFLFAGLLLFGWGALFGLYHWAKSVLTG
                     VATPTGTIMLAVLPLILGFQLILQAIVLDIQETPK"
     gene            1388..3112
                     /locus_tag="AC159P_01155"
     CDS             1388..3112
                     /locus_tag="AC159P_01155"
                     /inference="COORDINATES: protein motif:HMM:NF015983.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HIR3694085.1"
                     /translation="MTQDALSSIAFIHAPDSFLAETQQYGAMFMPVWAYTLAAYVEKP
                     ERYDLRLHDTRFDRAEDVPRADLFVFSGVNQDYETIVSTHAALRALHPDSVFVIGGPI
                     TWSLNQAGEGAKLEMFDHVFIGDGEEAFPRFLKMFAERQRLPRVLETKERFDVTRARG
                     FYRPFLDATFSRYYGGVLEVSRGCPFLCEFCDIRVLPDNNRPHNFAPEHIVAELDHMA
                     RLGIRQVLCAADNFIGDLRWAEEVLDRIIEWQERTGLRVALYTWLTINLSKHPRLLKK
                     MRQANFDMLFIGVESFSNNSLLETAKVQNTARDMVEALREIQSYGFAVVAGLIFGFDS
                     DTPDAFEMTLDGMSNAGLISGDPSLLTALPGTPLFRRMKLSGRLRNNKNSLGGYKYCT
                     NIRYLMPRDEMVAGYQRFVRRFCEGDYQYARLRSFLDNLDRGNYVPLNSEGYGSLGKY
                     ISMVFKSPGAVKMLLRRALQIASRPSVAWYSVRAFLLVLSRSRRHRRLFGVFQFWLFN
                     WTNAMLKYEGLTDADFDIESVPEGFDRSLILPEHYTDEAGEDIPQAKIAAQQRATVGQ
                     LRRLTVLQ"
     gene            3114..5429
                     /locus_tag="AC159P_01160"
     CDS             3114..5429
                     /locus_tag="AC159P_01160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694086.1"
                     /translation="MPPPPAISSTGARAPAPPRETGAAAPKARFPLRKLEFGDALLFA
                     YVAVFVRQCFWAVESDAAAWALTLAASALLGFAHLSAKPEVEGRTPRAFWLIVGLPLL
                     LVFAARVAFPDLAFDVLNHRLIQAERSLRGPQLGAGDFFPTIFPFNPAPDMLTGVFRH
                     LLGYRLGTIVNLLALVWAGTVLEKILRPFIAGASWRCLGVLLALFTEQALFQVNSYMV
                     DLLALPLVLEATRLALQLDESRTRTRDLLHAALLLGGATALKLTNAAMAVPVLFVFAV
                     RVFWPRRGAEARGAKTAGVVLLSAVFFLLPLLPHALYIYGETGNPFFPLYNNLIQSPL
                     WPHMTPYDGRWGPRDLRETLLWPLLAVTEQGRLSELGVYSGRLTLAFPAALVCLLFPR
                     AATRARLLALVVLLGSLLWSATSGYVRYAIFMEVLGGVLLLYLARYAATRAALWPRPL
                     RLAAAALPLCLLGAQAAVAASHVSETEWAKRPTVFEEAGAFRRELRWVWRDRALLDFQ
                     TAEGRALFGQVDAWVVSGVKTNGVAVLLRPDVPMLAVNNLEYFDRPASRRRLTGALER
                     LRGRRVYSLCLTEDLASSLDLLRRRRLVPGRSHRVIVPFFSARTRLDMTLVEVGLPEK
                     RETPRKAPGQPAVTEATAPLDDDAFLAGISVADAPAVLRAGARADLRVAVKNLSEFVW
                     PARGRADYAFLITVSNAWFDAGGSLVDNTDGKTGLPRDLWPGEEAEVTLAVTAPAEPG
                     EYILEIDLVQEGIAWFKDKGSQAWRARVKVE"
     gene            5483..>5803
                     /locus_tag="AC159P_01165"
     CDS             5483..>5803
                     /locus_tag="AC159P_01165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694087.1"
                     /translation="MNFRITLGALLMLAALTIVGCGPEAENTNAGGQPTPAGPKQAAA
                     LPENGFKGQLTLAAAPARLRAGQKETIQVKVRNASDARWYARGGEVNTHPDNRFYLAV
                     GNRWL"
BASE COUNT          834 a         2127 c         1884 g          958 t
ORIGIN      
        1 gccctcgctc ggcgcagacc ccggcgagtt cgctttcggg ctgcgctcgc gctggctcga
       61 cgcgagcggc gagcggacgg tgagcatcgt cggcgacatc ccgcacctcg cttacgacct
      121 cgcgcccggc gagaccgtac ccgtgccgct caccctcgcc ccgcccgccg cgcccggcga
      181 ctacctgctc gaagtccaca tgctccacga aggcgtcggc ttgttcctcg accgcggctc
      241 gcaaccgctc cgcctcgccg tgcgcgtcgc gcgctgagcg gcggtcagaa gggggggggt
      301 ggcattccgc gacgcgaggt gttagcttct acgactccgc cgagcatgta caagaaccgc
      361 cgcatcgccg tggtcgtccc ggcctacaac gaagagcgcc acgtcggcga ggtgctccgc
      421 ggcgtgcccg actacgttga ccacgtcatc gccgtggacg actgtagcgc cgacggcacg
      481 cgcgcggcca tcgagtcgtg cgacgacgcg cgcgtcgtcg ccctgtgcac gcccgcgaac
      541 ctcggcgtcg gcggcgcgac gttgctcggc taccggaagg cgctcgaact ggggtgcgac
      601 gtggcggtga agatggacgg cgacgggcag atgccgcccg cccacctgcc gctgctgctg
      661 gacgccgtcg tcgagcacgg ctacgactac gccaagggca accgcttcct cgcgggccgc
      721 tcgctggccg agatgccgaa ggtcaggctc ttcggcaacg tcgtgctcac cttcgtcaac
      781 aagctggcct ccggctactg gcacatcttc gacccgcaga acggctacac ggcggtcacg
      841 gcggaggcgc tccgccgcct cgacttcggc tcgctccacc ggcgctactt cttcgagaac
      901 gacatgctca tcgcgctcaa cttccacaac gcgcgcgtgc gcgacgtggc catccccgcg
      961 cgctacggcg acgagcgctc cgacctcaac ccgttcaagg tcggcgtgac gttcccgctc
     1021 ctgttcatcc cgcgcttctg gcggcgcatc taccgcaagt acgtgctgca cgacttctcg
     1081 cccgtcgcgc tcttcctctt cgcggggctg ctgctgttcg ggtggggcgc cctcttcggt
     1141 ctctaccact gggccaaaag cgtcctcacc ggggtggcga cgcccacggg gacgatcatg
     1201 ctcgcggtgc tgccgctcat cctcggcttc cagctcatcc ttcaggccat cgtgctcgac
     1261 atacaggaga cgccgaagtg aggcgcgctg ggccgaccct tcggggacgc ccgcttttac
     1321 aagccgccgc cgcttggtta tattagtacc cgttcccgcc cgcaccgggc cgtaagcaag
     1381 caccattatg acgcaggacg ccctcagcag catcgccttc atccacgcgc cggactcctt
     1441 tctcgccgag acgcagcagt acggcgcgat gttcatgccc gtctgggcct acacgctcgc
     1501 cgcctacgtc gagaagccgg aacgctacga cctgcggctc cacgacacgc gcttcgaccg
     1561 ggccgaggac gtgccgcgcg cagacctctt cgtcttcagc ggcgtcaacc aggactacga
     1621 gaccatcgtc tcgacgcacg cggccctgcg cgccctccac cccgactccg tcttcgtcat
     1681 cggcggcccc atcacctggt cgctcaacca ggcgggcgag ggcgcgaagc tggagatgtt
     1741 cgaccacgtc ttcatcggcg acggcgagga ggcgttcccg cgcttcctca agatgttcgc
     1801 cgagcggcaa cggctcccca gagtgctcga aaccaaggag cgcttcgacg tgacgcgggc
     1861 gcgcggcttc taccgcccgt tcctcgacgc gaccttcagc cgctactacg gcggcgtgct
     1921 cgaagtctcg cgcggctgcc cgttcctctg cgagttctgc gacatccgtg tgctgccgga
     1981 caacaaccgc ccgcacaact tcgcgcccga gcacatcgtc gcggaactcg accacatggc
     2041 gcggctcggc atccggcagg tcttgtgcgc cgccgacaac ttcatcggcg acctcaggtg
     2101 ggccgaggag gtgctcgacc gcatcatcga gtggcaggag cgcacgggcc tgcgggtcgc
     2161 gctctacacg tggctgacga tcaacctctc gaagcacccg cggctgctga agaagatgcg
     2221 gcaggcgaat ttcgacatgc tcttcatcgg cgtcgagagc ttcagcaaca actctttgct
     2281 ggagacggcg aaggtgcaga acacggcgcg cgacatggtc gaggcgctgc gcgagataca
     2341 gtcgtacggc ttcgcggtcg tcgccggtct catcttcggc ttcgactcgg acacgcccga
     2401 cgccttcgag atgacgctcg acgggatgag caacgcggga ctcatcagcg gcgacccgtc
     2461 gctgctgacc gcgctgccgg gcacgccctt gtttcgccgc atgaaacttt cgggccgcct
     2521 gcgcaacaac aagaacagcc tcggcggcta caagtactgc accaacatcc gctacctgat
     2581 gccgcgcgac gagatggtcg ccggctacca gcgtttcgtc cggcgcttct gcgaaggcga
     2641 ctaccagtac gcgaggctca ggagctttct cgacaacctc gaccgcggca actacgtgcc
     2701 gctcaactcc gaagggtacg gcagcctcgg caagtacatc tcgatggtct tcaagagtcc
     2761 gggcgccgtc aagatgctgc tgcggcgcgc gctccagatc gcttcgcgcc catcggtcgc
     2821 gtggtactcg gtgcgcgcgt tcctgctcgt gctctcgcgc tcgcgccgcc accggcggct
     2881 gttcggcgtc ttccagttct ggctcttcaa ctggacgaac gcgatgctga agtacgaggg
     2941 cctgacggac gccgacttcg acatcgagtc ggtgcccgaa gggttcgacc gctcgctcat
     3001 cctgcccgag cactacaccg acgaggccgg cgaggacatc ccgcaggcca agatcgcggc
     3061 ccagcagcgc gccaccgtcg gccaactgcg ccgcctgacc gtcctgcaat aacttgcctc
     3121 cgccacccgc catttcgtca acgggcgcgc gcgcgcccgc gccgccgcgc gagacgggcg
     3181 ccgccgcgcc gaaggcgcgc tttccgctcc gcaagctgga gttcggcgac gcgctcctct
     3241 tcgcttacgt cgccgtcttc gtgcggcagt gcttctgggc cgtcgagtcg gacgccgccg
     3301 cgtgggcgct caccctcgcc gcgtccgcgc tcctgggctt cgcgcacctc agcgcgaagc
     3361 cggaggtcga agggcgcacg ccccgcgctt tctggctgat cgtggggctg ccgctgctgt
     3421 tggtcttcgc ggcgcgcgtc gccttccccg acctcgcgtt cgacgtgctc aaccaccggc
     3481 tgattcaggc cgagcgctcg ctccgcgggc cgcaactcgg cgcgggtgac ttcttcccga
     3541 cgatcttccc cttcaacccc gcgccggaca tgctgaccgg cgttttccgc cacctcctcg
     3601 gctaccgcct cggtacaatc gttaacctgc tcgcgctcgt ctgggccggg acggtgctgg
     3661 agaaaatcct gcgccccttc atcgcggggg cttcgtggcg ctgcctcggc gtgctgctcg
     3721 cgctcttcac cgagcaggcg ctgttccagg tcaacagcta catggtcgac ctgctggcgc
     3781 tcccgctcgt gctcgaagcg acgcggctcg cgttgcagct cgacgagtcg cgcacgcgga
     3841 cgcgcgacct gctccacgcg gcgttgctgc tcggcggggc gacggcgctc aagctgacga
     3901 acgcggcgat ggccgtccct gtcctcttcg tctttgccgt ccgcgtcttc tggccgcggc
     3961 gcggcgcgga ggcgcgcggg gcgaagacgg cgggcgtcgt cctgctctcg gcggtcttct
     4021 tcctcctccc gctgctgccc cacgcgctct acatttacgg cgagacgggg aaccctttct
     4081 tcccgctcta caacaacctc atccagtcgc cgttgtggcc gcacatgacg ccctacgacg
     4141 ggcgttgggg gccgcgcgac ctgcgcgaga cgctgctgtg gccgctgctc gccgtgaccg
     4201 agcaggggcg gctctccgag cttggcgtct actccggtcg cctcacgctc gcgtttccag
     4261 ccgccctcgt ctgcctgctg ttcccgcgcg ccgcgacgcg cgcacggctg ctcgcgctcg
     4321 tcgtgctgct cggctccctg ctgtggagcg cgacgagcgg ctacgtccgt tacgcgatct
     4381 tcatggaggt tctgggcggc gtgctgctgc tctacctcgc gcgctacgcg gcgacacgcg
     4441 ccgccctctg gccgcgcccg ctgcggctcg cggcggcggc gctcccgctg tgcctgctcg
     4501 gcgcgcaggc cgccgtcgcc gcgagccatg tcagcgagac cgagtgggcc aagcgcccga
     4561 cggtcttcga agaggcgggc gcgttccgcc gcgagctgcg ctgggtctgg cgcgaccgcg
     4621 cgctgttgga cttccagacc gcggaggggc gtgcgctctt cgggcaggtg gacgcgtggg
     4681 tcgtgagcgg tgtgaagacc aacggcgtcg cggtgctgct gcggccagac gtgccgatgc
     4741 tcgccgtcaa caacctcgaa tacttcgaca ggccggcgtc gcggcggcgc ctgaccggcg
     4801 cgctcgaacg cctgcgcggg cggcgcgtct attcgctgtg cctgacggaa gacctcgctt
     4861 cgtcgctcga cctcctccgg cgccgccggc tcgtgccggg ccggtcgcac agagtcatcg
     4921 tccccttctt ctctgcgcgc acgcggctcg acatgacgct cgtcgaggtc gggctgccgg
     4981 agaagcgcga gacgccgcgc aaggcgccgg gccaacccgc ggtgaccgag gcgaccgcgc
     5041 cgctcgacga cgacgccttc ctcgcaggca tctccgtggc ggacgcgccc gccgtcctgc
     5101 gcgcgggagc gcgcgccgac ctccgcgtcg cggtgaagaa cctgagcgag ttcgtctggc
     5161 ctgcgcgcgg gcgcgcggac tacgccttcc tcatcacggt gtcgaacgcc tggttcgacg
     5221 cgggcgggtc gctcgtggat aacacggacg gcaagacggg cttgccgcgc gacctctggc
     5281 cgggcgagga ggccgaggtc acgctcgccg tcaccgcgcc cgccgaaccg ggcgagtaca
     5341 tcctcgaaat agacctcgtg caggaaggca tcgcgtggtt caaggacaaa ggctcgcagg
     5401 cttggcgcgc gcgtgtaaaa gtcgaataat gcgtaggccg cgaacaggct atttcagaca
     5461 accatcgaga ggacgagaac agatgaactt cagaataacg ttgggcgcgc tgctcatgct
     5521 ggccgcgttg accatcgtcg gatgcgggcc ggaggcggag aacacgaacg cggggggcca
     5581 gccgacgccc gccgggccga agcaggcggc ggcgctgccc gagaacggat tcaaggggca
     5641 gctcaccctc gccgctgcgc ccgcccgttt gcgcgccggc cagaaggaga ccatccaggt
     5701 caaggtcagg aacgcgagcg acgcgcgttg gtacgcgcgc ggcggcgagg tcaacacgca
     5761 ccccgataac cgcttctacc tcgccgtcgg caaccgctgg ctc
//
LOCUS       DBNGOH010000060         4461 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4408371, whole genome shotgun sequence.
ACCESSION   DBNGOH010000060 DBNGOH010000000
VERSION     DBNGOH010000060.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4461)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4461)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4461
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4408371"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..436)
                     /locus_tag="AC159P_01170"
     CDS             complement(<1..436)
                     /locus_tag="AC159P_01170"
                     /inference="COORDINATES: protein motif:HMM:NF019869.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HIR3694088.1"
                     /translation="MLNSEGAAVGQGVAGDPGGWALHDRWIVSRLNKTARAVDEALAA
                     YQFHEAVYALYHFFWDDFCDWYIELSKADVTAAEASPERDAARSRALTVLERALRLLH
                     PFMPFITEELWARTAEFGAKREGMLIVEAWPRLSDDLIDPAAE"
     gene            complement(437..2194)
                     /locus_tag="AC159P_01175"
     CDS             complement(437..2194)
                     /locus_tag="AC159P_01175"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HIR3694089.1"
                     /translation="MEIPKTYDPKQAEESHYARWEREGYFAPEINRDPSAPVYSIAIP
                     PPNVTGSLHMGHALQHTMMDVLVRHKRMSGYRTLWVTGTDHAGISTQLVVSRELKKEG
                     LTRHDLGREKFVERVWRWKAESGGAITRQMRREGASVDWSRERFTMDEGLSRAVREVF
                     VRLYEEGHIYRGARIVNWCPTDQTVLSDLEVIKETKKDGKLYFLQYPLKGSERRLTVA
                     TTRPETMLGDTAVAVNPSDERNKDLVGATVLLPLTRREIPVIADEYVDPAFGTGAVKI
                     TPAHDPADYEMGVRHDLAQVFVIDHNARMTRDAGAEFAALDRYEARAKVVARFEELGL
                     LEKIEDYEGAISKCERCKTVIEPLISTQWFVRMEELRDLALDLLRREGKPRFTPESYE
                     KVYTNWLENLRDWTISRQLWWGHQIPAWYDEEGNVHVARNEEEARAKAGTDKLTQDPD
                     VLDTWFSSALWPFSTLGWPGETADLRTFYPTSVLMTARDIIFLWVSRMVMMGMKFVGE
                     TPFHDVYITGTVLARDGQRMSKTKNNGIDPLDVFDKFGVDATRLVLASIGSTDTRWND
                     SQVESYRNFANKIWNATRF"
     gene            complement(2315..4144)
                     /locus_tag="AC159P_01180"
     CDS             complement(2315..4144)
                     /locus_tag="AC159P_01180"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3694090.1"
                     /translation="MVTGRRILLLADDSPATQKVVSLTFADEGLEVVAVGDGTEAARL
                     LEEGLTPDVVLADVIMPGLTGYELCERIKGDGRLARVPVVLLVGTFEPFNEAEARRVG
                     ADTVLTKPFQSIRDLVSKVGSLLGGSKPEAEDETTRPAAPPQEASQMEAPPQEAWRQE
                     SPPLQQDEASSSFADLGLDDEMIEARPAENYAETQAAPHAPFAAHAEAEGARAEADAA
                     QAPVAAERAHDSFADTLRDPFAVVTPRAADEWRGEAERRDEAQVFAETQTSFARVPVA
                     EEARAPQPAFAARVASASAADDALLDLDLDAPPSVSEADDFILDLGDEDERPVSHSAA
                     PVEAAAPPPHTAGAFAEAAHGETVQDSRAAVQQLQDMQAEHEHMATEVVAQDEPHAPA
                     VGHRLSDEREFADVQDFGSHTAREVLSAAVGEEAGASSHEAAVAPSFEETGEATSAPR
                     GFVEPEVVPAGEASPAVVEAEFTDGSVEGDVAKPPTGGFVETPFEAEAAPVIEEAAPV
                     VESAAVESAASTHVAESSSAVRESPFPPAPSTAGYEMGAARAGVEEGVRPEQLSPEVL
                     DAIARRVVELMSDKVVREIAWEVVPDLAELLIKQKIDEDSRRQ"
BASE COUNT          667 a         1547 c         1467 g          780 t
ORIGIN      
        1 cttccgcagc agggtctatc agatcgtcgc tgagacgcgg ccaggcttcg acgatcagca
       61 tgccttcacg ctttgcgccg aactcagcgg tgcgcgccca cagctcctcc gtgatgaagg
      121 gcatgaaggg gtggagcaga cgcagcgccc gttcgagcac cgtgagcgcg cggctgcgcg
      181 cggcgtcgcg ctcgggactc gcttcggcgg cggtcacgtc cgctttcgac agctcgatgt
      241 accagtcgca gaagtcgtcc cagaagaagt ggtagagcgc gtagaccgcc tcgtggaact
      301 ggtaagcggc gagcgcctca tcgacggcgc gcgccgtctt gttcaggcgc gagacgatcc
      361 agcggtcgtg cagcgcccag ccgccggggt cgcccgcgac gccttggccg accgccgcgc
      421 cctccgagtt cagcagtcag aagcgcgtcg cgttccagat tttgttggcg aagttccgat
      481 aggactcgac ctgactgtcg ttccagcgcg tgtccgtgct cccgatggag gcgagcacga
      541 gccgcgtcgc gtccacgccg aatttgtcga acacgtcgag cgggtcgatg ccgttgttct
      601 tcgtcttcga catccgctgc ccgtcccgcg cgaggacggt gccggtgatg tacacgtcgt
      661 ggaagggcgt ctcgccgacg aacttcatcc ccatcatcac catccgcgac acccacagga
      721 agatgatgtc gcgcgcggtc atgagcaccg aggtcgggta gaacgtgcgg aggtctgcgg
      781 tctctccggg ccagcccaaa gtcgagaaag gccacagcgc cgaagagaac cacgtgtcga
      841 gcacgtccgg gtcttgcgtc agcttgtccg tccccgcttt ggcgcgcgcc tcttcctcgt
      901 ttcgcgcgac gtggacgttg ccctcctcgt cgtaccacgc ggggatttgg tggccccacc
      961 agagctggcg cgagatcgtc cagtcgcgca ggttctcaag ccagttcgta tagaccttct
     1021 cgtaagattc gggcgtgaag cgcggcttgc cctcccggcg caaaaggtcg agcgccaggt
     1081 cgcgcagctc ttccatgcgg acgaaccact gcgtcgagat cagcggctcg atgaccgtct
     1141 tgcagcgctc gcacttcgag atggcgccct cgtagtcctc gattttttcg agcaggccca
     1201 gctcttcgaa acgggcgacg accttcgcgc gcgcctcgta gcggtcaaga gctgcgaact
     1261 cggcgcccgc gtcccgagtc atccgcgcgt tgtggtcgat gacgaagacc tgcgcgaggt
     1321 cgtggcgcac gcccatctcg tagtccgcag gatcgtgcgc gggcgtgatc ttgacggccc
     1381 cggtgccgaa agcgggatcg acgtactcgt ccgcgatgac cggaatctcg cgcctcgtca
     1441 aaggcagcag gacggtcgcg ccgacgaggt ctttgttacg ctcgtccgaa gggttgaccg
     1501 cgaccgccgt gtcgccgagc atcgtttcgg gccgggtggt cgccaccgtc aggcgccgct
     1561 cgctcccctt cagcgggtat tgcaggaagt agagcttgcc gtccttcttc gtctccttga
     1621 tgacttccag atcggagagc acggtctggt cggtcgggca ccagttgacg atgcgcgcgc
     1681 cgcgatagat gtgcccctct tcgtagaggc ggacgaagac ctctcgcacg gcgcgcgaca
     1741 gcccctcgtc catcgtgaag cgctcgcgcg accaatcgac gctcgcgccc tcgcgccgca
     1801 tctgccgcgt gatggcgccg cccgactcgg ccttccaacg ccacacgcgc tcgacgaact
     1861 tctcgcgccc gaggtcgtgc cgcgtcagcc cttccttctt caactcgcgg ctgacgacga
     1921 gctgcgtcga gatgcccgcg tggtcggtgc ccgtgaccca gagcgtgcgg tagcccgaca
     1981 tgcgcttgtg ccggacgagc acgtccatca tcgtgtgctg caaggcgtgg cccatgtgga
     2041 gcgaccccgt cacgttcggc ggcgggatgg cgatcgagta gaccggcgcc gacgggtcgc
     2101 ggttgatctc aggcgcgaag tagccctcgc gctcccagcg cgcgtagtgg ctctcctcgg
     2161 cctgtttcgg gtcgtaagtc ttcggaatct ccatacccgg acaatactaa cagccgctcc
     2221 gcggaaggca aaaggcaaaa ggcaaaaggc aaaagtgaca agggttgcgc cctccacttt
     2281 tgccttttgc cttttgcctt ttgccttccg gcggctactg tcgccggctg tcctcgtcga
     2341 tcttctgctt gatgagcagc tccgcgaggt cggggacgac ctcccacgcg atctcgcgga
     2401 cgaccttgtc cgacatcagc tcgacgacgc ggcgcgcgat ggcgtcgagc acttcgggcg
     2461 agagctgttc ggggcgcacg ccttcttcga cgccggcgcg cgccgcgccc atctcgtagc
     2521 ccgcggtcga gggcgcgggc gggaacggcg attcgcgcac ggcggaagaa ctttcggcga
     2581 cgtgcgtgga agcggcactc tcgacagcgg cactctcgac aaccggcgcg gcttcctcaa
     2641 tcacgggcgc ggcttcggcc tcgaaggggg tctcgacgaa gccgcccgtc ggcggtttcg
     2701 ccacgtcgcc ttcgacgctg ccgtccgtga actcggcctc gacgaccgcg ggcgacgctt
     2761 cgcccgcagg gacgacctcc ggctcgacga acccgcgcgg cgcgctcgtc gcttcgcccg
     2821 tctcttcgaa cgacggcgcg accgccgcct catgcgaaga agcgcccgcc tcctcgccca
     2881 ccgccgcgct caacacctcg cgcgccgtgt ggctgccgaa atcctgcacg tccgcgaact
     2941 cgcgttcatc acttaaccgg tgcccgacgg cgggcgcgtg tggctcgtcc tgcgcgacca
     3001 cttcggtggc catatgttcg tgctcggcct gcatatcctg caactgctgg acggcggcgc
     3061 ggctgtcctg caccgtctcg ccgtgcgcgg cctccgcgaa ggcgcccgcc gtgtggggcg
     3121 gcggcgccgc cgcctcgacg ggcgcggcac tgtgggacac gggccgctcg tcctcgtcgc
     3181 cgaggtcgag gatgaagtcg tcggcctcgc tcactgaagg cggcgcgtcg aggtcgaggt
     3241 cgagcagcgc gtcgtccgcg gctgaggctg aggccacgcg ggcggcgaac gcgggctgcg
     3301 gcgcgcgcgc ctcttcggcc acggggacgc gcgcgaaaga agtctgtgtt tccgcgaaga
     3361 cctgcgcctc gtcgcggcgc tcggcttcgc cgcgccactc gtccgcggcg cgaggggtga
     3421 cgacggcgaa cgggtcgcgc agcgtgtcgg cgaacgaatc gtgcgccctc tccgccgcga
     3481 ccggcgcctg cgcggcgtcc gcttcggcgc gcgcgccctc ggcttcggcg tgcgcggcaa
     3541 agggggcgtg cggcgcggct tgcgtctccg cgtagttctc cgccgggcgc gcttcgatca
     3601 tctcgtcgtc caacccgagg tcggcgaacg acgacgacgc ctcatcctgc tgcagcggcg
     3661 gcgactcctg ccgccaggct tcctgcggcg gcgcttccat ctgcgaggct tcctgcggcg
     3721 gcgccgcggg gcgggtggtc tcgtcttcgg cctccggctt ggagccgccg aggagcgacc
     3781 cgaccttgct cacgaggtcg cgtatggatt ggaatggttt ggtcaagaca gtgtccgcgc
     3841 cgactctgcg cgcctccgct tcgttgaaag gttcaaacgt gccgacgagc agcaccacgg
     3901 ggacgcgggc cagccgcccg tcgcccttga tgcgctcgca cagttcgtag cccgtgagtc
     3961 cgggcatgat aacatccgcc agcacaacgt caggcgtcag cccctcttcg agtaaccggg
     4021 cggcttccgt gccgtcgccg acggcgacga cctccagccc ctcgtccgcg aaggtcaggc
     4081 tgacaacttt ttgcgtggcg ggtgaatcgt ctgcgagaag cagaatccgg cgtcccgtta
     4141 ccaaaagaac cctctcctca tgtaagggag ccaatccgtc aggggggttg ttcacatccg
     4201 aaacgcatgc gcgtccaagg acggagtggc gaggcaagcg gcgctcccgg acaaccactt
     4261 cgtcggaagc cacattcaaa cctaacatct gcgccgagag cgcgtcaaga tgattttccg
     4321 ccgcgccgcg ccgccatatt cgagggcgcc gccgcgcgta atgccgaccc cggagtcggc
     4381 agactgtccg ccctcgcctc cgcaaatttc tgttgggcgt cgtcccgcaa gcggcgcata
     4441 atgcgtgcgt tcttacaccc g
//
LOCUS       DBNGOH010000061         9419 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4042068, whole genome shotgun sequence.
ACCESSION   DBNGOH010000061 DBNGOH010000000
VERSION     DBNGOH010000061.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 9419)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9419)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9419
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4042068"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3329)
                     /locus_tag="AC159P_01185"
     CDS             complement(<1..3329)
                     /locus_tag="AC159P_01185"
                     /inference="COORDINATES: protein motif:HMM:NF051698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR3694091.1"
                     /translation="MRATTNRIIVSTLLLLLCLAAQTLAQTATTATITGLVTDAQGAA
                     VAGASATLTDKATGQERTVVTDAEGKYAFPNLNAGTYDLKITATGFGPKTFADIKADV
                     TKTITQDVALEIGAVTEQVVVTAGEQALLLNQDATIGNTIESRRITVLPNINRDVTEL
                     LALQPGISTTGEATGARSDQSTLALDGIDVTDQNLGQAFRSVIPTPNDAIEEFRVSTS
                     NPNATFGRSSGAQAVFVTKRGGNDWHGSFYEYHRNSALNANTWHNNRTGVPRAFLIDN
                     RFGGTVSGPVFADKTFFFFLLEGRRNVSSTTATRLVPTATLKQGLLRFRDAGGAVQTI
                     DPQTLDPRGLGASPVVLQLLNLFPAPNDLSQGDGLNTAGLTRNIPTSQNSELGILRVD
                     HTFNDRWSFDGSFKAFREIQTLAGNATQVDIANGRTTAISPQRPRSLSLGLTGVLTSN
                     ITNEVRVGWVHDRLENNREAPQPQVPGLNIALDLAGALLDEPVDVDTQRTRTQARTMN
                     TYQFIDNLTWTKNTHTIQAGFNMRHINSFDLRVDKVVGSIASPVAALGSVANNAIPAA
                     QRPSFIAAGDVARYNQLYAALLGQVETITYLATRNGQLQPNPIGTPLITNSTLNAYEF
                     YGADNWRVTPTLTVSYGVMYSWQKPPIEDEGKQTVAVFQGTNELINPKDYLRQKFDAA
                     ARGEIFNPDIAYIPINEANRSGVFDVDWSNISPRISVAWSPSRSGGILGKLFGQGETV
                     LRGGYSLLYDRQNTVQTITIPTLGVGFAQTVAINNALNGAGQRFRAGVDGPLPLPAPT
                     GATSPIVPAKPFGETLSFVVDPFSTVPRSHTINFTVQRSLPGKMVLEVGYAGRLGRNL
                     YQSLNLNQVPYNFKDNLSGQTFAEAFDALAAQIRGGTAASAVTAQPWFENLLVNLAPA
                     NGSRTRALAAAQTANIINGNLNNLFVGALDVFAAQPFNNRQSNELFYRTSIGRSNYHA
                     LLATLRKRVSQGLSFDLNYTLSRSLDQAGAVQNTANIQPNSFDPDAEYVPSAFDQTHN
                     FNANYVYDLPFGRGRRFLGGASGFADKLLSGWFTSGIFRAVSGTPIYIQQGTQVWGGS
                     QLLGFGSGAILLPGADFGTNV"
     gene            complement(3352..3771)
                     /locus_tag="AC159P_01190"
     CDS             complement(3352..3771)
                     /locus_tag="AC159P_01190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694092.1"
                     /translation="MRSNVSSLFLYPLMARETPAARGAVALLDRHHFGFYLATWIKNQ
                     KLESANPKTCKVGSPKTDSASRPNARAENRPTLPAPAEGCASLNLNPARATFYSTSGG
                     RAPGSRTGVARVRGLKGVASGIDLALPDPRPRLVSLL"
     gene            complement(3896..5941)
                     /locus_tag="AC159P_01195"
     CDS             complement(3896..5941)
                     /locus_tag="AC159P_01195"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF017464.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HIR3694093.1"
                     /translation="MRFFKVRFAAGSVLALVLCLAVVAQSGGAGRGFDVSRMDKTASA
                     CQDFYQFSNGNWLKTTEIPPAFSSWGSFNILAENNRNTLREILDAAAKTTDAKKGSVE
                     QKIGDFYASCTDEAKREAEGVKPLLPEFARIEKIKSAAELQAQVARMHSMGVNALFDF
                     EALPDFKNSSVVMGIATQGGLSLPTNEYYTKTDEKSVDIRNKFVQHVTNMFQLLGDAP
                     EKAAANAKTVLAIQTRLAENSRDPVALRDISKQYNKMTAKELQALTPNLSWGDYFAAL
                     GQPRDLTINLAHPEFYQNVNKMLTEVPIEDWKTYLRWHLISAAAPSLSSKFEDESFNF
                     NGKVLAGRKEQNPRWRRCVAATDAQLGEALGQAYVARAFTPESKRRMQTMINNLIAAF
                     RERVQKLDWMSDPTRKEALAKLAAFQQKIGYPDKWIDYSALEISRASFVENALRAGQF
                     EVRREMAKIGKPVDRGEWGMTPPTVNAYNNWLMNEIVFPAGILQAPFFNPEADDAINY
                     GAIGAVIGHEITHGFDDQGANFDLQGNLKDWWTPDDKKKFQERAECIVNQFGAYEVEP
                     GLKMTGKLVSGESIADLGGLQVAYDAFLKSLEGKPRPKDIDGFTPEQRFFMGWAQVWA
                     SKYTPEITRLITQSDPHPISRFRVNGPLSNMPEFAAAFQCKAGDPMVRPEKDRCKIW"
     gene            complement(6231..8291)
                     /locus_tag="AC159P_01200"
     CDS             complement(6231..8291)
                     /locus_tag="AC159P_01200"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HIR3694094.1"
                     /translation="MSSSIFRRAGLPLLLAAVCLAAVAASAGSRSPLLSRGFDPANLD
                     TSCEPCKDFYQFANGGWLAKNEIPSTHSVWGTTSGMSEKNVETLRLILEEAAKNKAAR
                     AGTNEQRLGDFYAACMDEAGIEAAGSKPLAPALAEIAKLKDAKALPALLGWLHTEGVA
                     GVFGFGIGPDAKESRQSLAGLGQGGLSLPDRDYYLKDDERSKTLRAAYQTHVTNLFKL
                     LGDAPDKAAANAETVLKIETELARVSLDRVARRDPLKQYNKMSPAEAQKLVPAFDLAA
                     YFKAMGAPAIAEVNVGQPDFFRGLDKVLASVPAEEWQTYLRWHLVRGAAPALSSAFVN
                     ESFEFNGKTLTGTKEMLPRWRRCVNATDNALGEALGEAFVKKAFPPESKARMRELVKN
                     LIAALREDIGTLEWMSEPTRKAAIAKLSAFNTKIGYPDKWRDYTQYKVERASYVDNLR
                     GSARFERRRNLAKLGKPVDRTEWLMTAPTVNAYHYPVFAEIVFPAGILQPPLFNPEAD
                     DAINYGAIGGVIGHELTHGFDDQGRKFDLNGNLADWWTEADGKAYDARARCVEEQFAS
                     FEVEPGLKLNGKLVLGESIADLGGLKIAWLAFQKSLEGKPRPASIDGFTPEQRFFLGW
                     AQGWGRKYRPEELRRRTQVDPHPPGKFRTNGPLSNMPEFAAAWSCKVGDAMVRPPENR
                     CQVW"
     gene            complement(8339..>9419)
                     /locus_tag="AC159P_01205"
     CDS             complement(8339..>9419)
                     /locus_tag="AC159P_01205"
                     /inference="COORDINATES: protein motif:HMM:NF044870.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YVTN family beta-propeller repeat protein"
                     /protein_id="HIR3694095.1"
                     /translation="LFAAAALVLVIITDPSHAPSARAQQGQAASPAASSASYLLVLNK
                     AEASVTIIDPVKMEAVGRVAVGEGPHEVVTSADGRTAYVSNYGGQKPGNSISIIDLAA
                     RKETKRVDLGALWRPHGLVEREGKIYFTAEVNRAVARYDPASDKVDWVMGLGEKGTHM
                     LVFHPTRKTIYTANIGSDTASVIELDKPSQPGPPPRVTHVPVGKQPEAIDISPDGREV
                     WVGHNADGGVSIIDTETSKVKETIKVGGMPIRVKFTPDGKRVLVSSPPTGELTILDAA
                     TRKELKRIKLGTPPPQGEPAPTGQIPIGVLVAPDSRSAFVALTVVVGGQPAAGKVARI
                     NLESMEVAGSVETGQGPDGLGWAGK"
BASE COUNT         1392 a         2895 c         3340 g         1792 t
ORIGIN      
        1 ttgacgttgg tcccgaagtc ggcccccggc agcaggatcg cgccggagcc gaaaccgagg
       61 agctggctcc cgccccacac ctgcgtcccc tgctggatgt agatcggcgt cccgctgacg
      121 gcgcggaaga tgccggaagt gaaccagccg gagagcagtt tgtccgcgaa cccgctcgcg
      181 ccgccgagga agcgccggcc cctgccgaac gggaggtcgt agacgtagtt ggcgttgaag
      241 ttgtgcgtct ggtcgaacgc cgacgggacg tactcggcgt ccgggtcgaa ggagttcggc
      301 tggatgttcg ccgtgttctg caccgcgccg gcctggtcga gcgagcgcga gagcgtgtag
      361 ttgaggtcga agctcagccc ctgcgagacg cgcttgcgca aggtggcgag cagcgcgtgg
      421 tagttcgagc gaccgatgga ggtgcggtag aacagctcgt tggactggcg gttgttgaac
      481 ggctgcgcgg cgaagacgtc gagcgcgccg acgaacaggt tgttcaggtt gccgttgatg
      541 atgttggcgg tctgcgccgc cgccagcgcc cgcgtgcggc tcccgttggc gggcgcaagg
      601 ttgacgagca ggttctcgaa ccacggctgc gccgtgacgg cggacgccgc cgtgccgccg
      661 cggatctggg ccgccagcgc gtcgaaggct tcggcgaacg tctgcccgga gaggttgtcc
      721 ttgaagttgt agggcacctg gttgaggttg aggctctggt acaggttgcg cccgaggcgg
      781 cccgcgtagc cgacttcgag caccatcttg ccgggcagcg agcgctgcac ggtgaagttg
      841 atggtgtggc tgcgcgggac ggtgctgaac ggatcgacga cgaacgagag cgtctcgccg
      901 aacggcttgg ccgggacgat gggcgaggtg gcgccggtcg gcgcgggcag cggcagcggc
      961 ccgtcaacgc ccgcgcggaa gcgctgcccg gcaccgttca gcgcgttgtt gatggcgacc
     1021 gtctgggcga agccgacgcc gagcgtcggg atggtgatcg tctggacggt gttctgccgg
     1081 tcgtagagca gcgagtagcc gccccgcagg acggtctcgc cctggccgaa cagtttgccg
     1141 agaatgccgc cgctgcgcga gggactccac gccaccgaga tgcgcggcga gatgttcgac
     1201 cagtcaacgt cgaacacgcc gctgcggttc gcctcgttga tcgggatgta ggcgatgtcc
     1261 gggttgaaaa tctcgccgcg cgcggcggcg tcgaacttct gccgcaagta atctttcggg
     1321 ttgatcagct cgttggtgcc ctggaagacc gccaccgtct gcttgccttc gtcctcgatg
     1381 ggcggcttct gccaggaata catcacgccg tagctgacgg tcagcgtcgg cgtcacgcgc
     1441 cagttgtcgg ccccgtagaa ctcgtaggcg ttgagcgtgg agttggtgat gagcggggtg
     1501 ccgatggggt tgggctgaag ctggccgttg cgcgtggcga ggtaggtgat ggtttcgacc
     1561 tgcccgagca gcgccgcgta gagctggttg tagcgcgcga cgtcgccggc ggcgatgaac
     1621 gaggggcgct gcgccgcggg gatggcgttg ttcgccaccg agccgagcgc ggcgaccggc
     1681 gaggcgatgg aacccaccac tttgtccacg cgcaggtcga acgaattgat gtggcgcatg
     1741 ttgaagccgg cctggatcgt gtgcgtgttc ttcgtccagg tgaggttgtc gatgaactgg
     1801 taggtgttca tcgtgcgcgc ctgcgtgcgg gtgcgctgcg tatccacgtc caccggctcg
     1861 tcgagcaacg cgcccgccaa gtcgagcgcg atgttgaggc cgggcacctg cggctgcggc
     1921 gcctcgcggt tgttttcgag gcggtcgtgc acccagccca ccctcacttc gttggtgatg
     1981 ttcgacgtga ggactccggt caggccgagc gagagcgagc gcgggcgctg cgggctgatg
     2041 gcggtggtgc gcccgttggc gatgtccacc tgcgtggcgt tgcccgcgag ggtctgaatc
     2101 tcgcggaagg ctttgaacga gccgtcgaaa ctccaacggt cgttgaaggt gtgatcgacg
     2161 cgcaggatgc cgagctcgct gttctggctg gtcgggatgt tgcgcgtgag tcccgcggtg
     2221 ttcaggccgt cgccctgcga caggtcgttc ggcgcgggga agaggttcag cagttgcagc
     2281 acgacggggc ttgcgccgag gccgcgcggg tcgagcgtct gcgggtcgat ggtctgcacg
     2341 gcgccgcccg cgtcgcggaa gcggagcagc ccctgcttga gcgtcgcggt cggcacgagg
     2401 cgtgtcgcgg tcgtcgagct gacgttgcgg cgcccttcga gcaggaagaa gaagaaggtc
     2461 ttgtccgcga agacggggcc gctcaccgtg ccgccgaagc ggttgtcgat gaggaaggcg
     2521 cgcggcacgc ccgtgcggtt gttgtgccac gtgttggcgt tgagtgcgct gttgcggtgg
     2581 tattcataga acgagccgtg ccagtcgttg ccgccgcgct tggtgacgaa gacggcctgc
     2641 gcgcccgacg agcgcccgaa cgtcgcgttc gggttcgacg tgctcacgcg gaactcctcg
     2701 atggcgtcgt tcggcgtcgg gatcaccgag cggaaggcct ggccgaggtt ctgatccgtc
     2761 acgtcgatgc cgtcgagcgc gagcgtggac tggtcggagc gggcgccggt cgcctcgccg
     2821 gtcgtcgaga tgccgggctg gagcgccagc agttcggtca cgtcccgatt gatattaggc
     2881 agcaccgtga tgcggcggct ttcgatggtg ttgccgatgg tcgcgtcctg attgagcagg
     2941 agcgcctgct cgcccgcggt gacgacgacc tgctcggtca ccgcgccgat ctccagcgcg
     3001 acatcctgcg tgatcgtctt ggtcacgtcg gccttgatgt cggcgaaggt cttcgggccg
     3061 aagccggtgg cggtgatctt caaatcgtac gtgccggcgt tcaggttggg gaaggcgtac
     3121 ttcccttccg cgtcggtgac gacggttcgt tcctggcccg tcgccttgtc cgtcagcgtc
     3181 gcgctggcgc cggcgaccgc cgcgccctgc gcgtcggtga cgaggccggt gatggtggcg
     3241 gtggtggccg tctgcgcgag cgtctgcgcc gcgagacaca ggagcaggag caaagtggag
     3301 acgataattc gattggtggt ggccctcatg ttgtcctccg agttgctacg ttcagagtaa
     3361 agagactagc cgaggtctgg gatcgggcag tgcaagatcg ataccagaag ccaccccctt
     3421 cagtccgcgc acgcgggcca cacccgtgcg acttcccggc gcgcgcccgc cggaggtcga
     3481 ataaaatgtt gcccgtgctg gattaagatt cagtgaggcg cagccctcgg cgggggcggg
     3541 aagcgtgggc cggttttcgg cgcgggcgtt ggggcgcgaa gccgaatccg ttttcggtga
     3601 tccgacttta caggttttcg gatttgccga ttcgagtttt tgatttttaa tccaagtcgc
     3661 caaataaaat ccaaagtgat gtcggtcgag aagggcgaca gccccccgcg cggcgggcgt
     3721 ctcgcgcgcc atcagcggat aaagaaagag gcttgagacg ttcgatctca agcctcctca
     3781 cttcgctgct tgtcgagcaa agaggcttgg gaggtaaagt cccaagcctc ttccgtctac
     3841 tgtcttttgt cttctgcttt aactgcctac cgcctactac ctactgccta ctgctttacc
     3901 aaatcttgca gcggtctttc tcggggcgca ccatcgggtc gccggccttg cactggaagg
     3961 cggcggcgaa ctcgggcatg ttcgagagcg ggccgttgac gcggaagcgc gagatcgggt
     4021 gcgggtcgga ctgcgtgatg aggcgggtga tctcgggcgt gtacttcgag gcccagacct
     4081 gcgcccagcc catgaagaag cgctgctcgg gcgtgaagcc gtcgatgtcc ttcgggcgcg
     4141 gcttgccttc cagagacttg aggaaggcgt cgtaggcgac ctgcaacccg ccgaggtcgg
     4201 cgatactttc gccggagacg agcttgcccg tcatcttcaa gcccggctcg acctcgtagg
     4261 cgccgaactg gttgacgatg cactcggcgc gctcctgaaa cttcttcttg tcgtcgggcg
     4321 tccaccagtc cttgaggttg ccttgcagat cgaagttcgc gccctggtcg tcgaagccgt
     4381 gcgtgatctc gtggccgatg accgcgccga tggcgccgta gttgatggcg tcgtcggcct
     4441 cggggttgaa gaagggcgct tgcaggatgc ccgcggggaa gacgatctcg ttcatgagcc
     4501 agttgttgta ggcgttgacg gtgggcggcg tcatgcccca ctcgccgcgg tctacgggct
     4561 tgccgatctt cgccatctcg cggcgcacct cgaactgccc ggcgcgcagc gcgttctcga
     4621 cgaacgaggc gcggctgatc tcaagcgccg agtagtcgat ccacttgtcg gggtagccga
     4681 tcttctgctg gaaggcggcg agcttggcga gtgcctcctt gcgcgtcggg tcgctcatcc
     4741 agtcgagctt ctgcacgcgc tcgcggaagg cggcgatgag gttgttgatc atcgtctgca
     4801 tccgccgctt cgactcgggc gtgaaggcgc gcgcgacgta agcctgcccg agcgcctcgc
     4861 ccaattgcgc gtcggtcgcg gcgacgcagc ggcgccagcg cgggttctgc tccttacggc
     4921 ccgccaagac cttgccgttg aagttgaagc tctcgtcctc gaacttcgag gagagcgacg
     4981 gggccgccgc cgagatgagg tgccaacgca ggtaggtctt ccagtcctcg atgggaacct
     5041 cggtcagcat cttgttgacg ttctggtaga actccggatg cgccagattg atagtcaggt
     5101 cgcgcggctg gccgagcgcg gcgaagtaat cgccccaaga gaggttcggc gtcagcgcct
     5161 gaagctcttt ggcggtcatt ttgttgtact gcttcgaaat gtcgcgcagc gcgacggggt
     5221 cgcgcgagtt ctcggcgagc cgcgtctgaa tcgccagcac ggtcttggcg ttggccgcgg
     5281 ccttttcggg cgcgtcgccg aggagctgga acatgttcgt cacgtgctgg acgaacttgt
     5341 tgcggatgtc aaccgacttc tcgtcggtct tcgtgtagta ctcgttggtc ggcagcgaga
     5401 ggccgccctg cgtggcgatg cccatcacga ccgagctgtt cttgaagtcg ggcagcgcct
     5461 cgaagtcgaa gagcgcgttc acgcccatcg agtgcatgcg cgcgacctgc gcctgtagct
     5521 ccgccgcgct cttgatcttc tcgatgcgcg cgaactcggg cagcagcggc ttgacgccct
     5581 cggcctcgcg cttggcctcg tccgtgcacg acgcgtagaa gtcgccgatc ttctgctcga
     5641 cgctcccctt cttggcgtcc gtcgtcttgg ccgccgcgtc cagaatctcg cgcagggtgt
     5701 tgcggttgtt ctcggcgagg atgttgaagc tgccccacga cgagaaggcg ggcgggattt
     5761 cggtcgtctt cagccagttg ccgttgctga actggtagaa gtcctgacac gccgaggcgg
     5821 tcttgtccat ccgcgacacg tcgaagccgc ggcccgcgcc gccgctctgg gcgacgacgg
     5881 cgaggcacaa cacgagcgcc agcaccgagc cggcggcgaa cctgacttta aagaaacgca
     5941 tgcggaaaac tccttgagcg gtctggtgat tgcggtcttt cggacgcacc cgaaactctg
     6001 caagccaaaa gtccgggccg cgcgcgcttg tgcaggggat gccttagttt ctcatgaatg
     6061 ccggtcgatt tccacttgag gaaaagtcgg aaggcggaac gcagaacgat gaatcattcc
     6121 acggttttct gtcttcagtg aagacacgtg ttcttgccga agactgaaaa ccgtagactg
     6181 aaaactgtct ttcattcacc gttccgcgtt ccgccttccg accttcttcg ctaccagacc
     6241 tgacagcggt tctccggcgg cctgaccatc gcgtcgccga ccttgcagct ccacgcggcg
     6301 gcgaactcgg gcatgttcga gagcgggccg ttggttctga acttgcccgg cgggtgcgga
     6361 tcgacctgcg tgcggcggcg cagctcttcg gggcggtact tgcgccccca cccttgcgcc
     6421 cagccgagga agaagcgctg ctcgggcgtg aagccgtcga tgctcgcggg gcgcggcttg
     6481 ccttcgagcg acttctggaa ggcgagccac gcgatcttga ggccgcccaa gtcggcaatc
     6541 gattcgccga gcacgagctt gccgttgagc ttcaggcccg gctcgacctc gaacgaagcg
     6601 aactgctctt cgacgcaacg tgcgcgcgcg tcgtaagcct tgccgtcggc ctccgtccac
     6661 cagtcggcga ggttgccgtt gaggtcgaac ttgcgcccct gatcgtcgaa gccgtgggtc
     6721 agctcgtggc cgatgacgcc gccgatggcg ccgtagttga tggcgtcgtc ggcctccggg
     6781 ttgaaaagcg gcggctggag gatgcccgcg gggaagacga tctcggcgaa gaccggatag
     6841 tggtaggcgt tgacggtggg cgccgtcatc agccattcgg tgcggtcaac cggcttgccg
     6901 agcttggcga ggttgcggcg gcgctcgaac cgcgccgagc cgcgcaggtt gtccacgtac
     6961 gaggcgcgct cgaccttgta ctgcgtgtag tcgcgccact tgtcggggta gccgatcttc
     7021 gtgttgaacg cggagagctt ggcgatggcc gccttgcgcg tcggctcgct catccattcg
     7081 agcgtgccga tgtcttcgcg cagcgccgcg atgaggttct tcaccagctc gcgcatccgc
     7141 gccttcgact cgggcggaaa agccttcttg acgaaagcct cgccgagcgc ttcgccgagc
     7201 gcgttgtccg tcgcgttgac gcagcggcgc cagcgcggga gcatctcctt cgtgcccgtc
     7261 agcgtcttgc cgttgaactc gaagctctcg ttgacgaacg ccgaagagag cgcgggcgcc
     7321 gcgccgcgca cgaggtgcca gcgcagatag gtctgccact cctcggcggg gacgctcgcg
     7381 agaaccttgt cgaggccgcg gaagaagtcc ggctgcccga cgttgacttc cgcgatggcg
     7441 ggcgcgccca tcgccttgaa gtaggcggcg aggtcgaagg caggcacgag cttctgcgcc
     7501 tcggcgggcg acatcttgtt gtactgcttc agggggtcgc ggcgcgcgac gcggtcaagc
     7561 gaaactcgcg ccagctcggt ctcgatcttg aggacggtct cggcgttcgc ggcggccttg
     7621 tcgggcgcgt cgccgagcag cttgaacagg ttcgtgacgt gcgtctggta ggcggcgcgc
     7681 agcgtcttcg agcgctcgtc gtccttgagg taatagtcgc ggtcggggag gctcaagccc
     7741 ccttgtccga gaccggcgag agactggcgg ctctccttgg cgtcggggcc gatgccgaag
     7801 ccgaagacgc cggccacgcc ttcggtgtgc agccacccga gcagcgcggg cagcgccttc
     7861 gcgtctttga gcttggcgat ctcggcgagc gcgggcgcga gcggcttact gcccgccgcc
     7921 tcgatgccgg cctcgtccat gcacgcggcg tagaagtcgc cgaggcgctg ctcgttcgtg
     7981 ccggcgcggg ccgccttgtt cttggccgcc tcttcgagaa tcaggcgcag cgtctcgacg
     8041 ttcttctcgc tcatcccgct cgtcgtgccc cacacggagt gggtcgaggg gatttcgttc
     8101 ttggcgagcc agccgccgtt ggcgaactgg tagaagtcct tgcacggctc gcaactggtg
     8161 tcgaggttcg cggggtcgaa gccgcggctc aagagcgggc tgcggctacc cgcgctcgcg
     8221 gcgaccgcag cgaggcacac ggcggccagc agcagcggca ggcccgcgcg gcggaaaatc
     8281 gaagatgaca tgggtggctc ctttaaagtg atcttaaccg tggttgaaaa gcacgccgtt
     8341 atttgccggc ccagccgagg ccgtccggcc cctggccggt ttcgacgctg ccggcgactt
     8401 ccatgctttc gaggttgatg cgcgcgacct tgcccgcggc gggctggccg cccacgacga
     8461 cggtgagcgc gacgaaggcg ctccggctgt cgggcgcgac gaggacgccg atgggtatct
     8521 gcccggtggg cgcaggctcg ccctgcgggg gcggcgtgcc gagtttgatg cgcttcagct
     8581 ccttgcgcgt cgcggcgtcg aggatggtca gttcgcccgt cggcgggctg gagaccagca
     8641 cgcgcttgcc gtcgggcgtg aacttgacgc ggatgggcat gccgccgacc ttgatcgtct
     8701 ctttgacctt gctcgtctcg gtgtcgatga tcgagacgcc gccgtcggcg ttgtggccga
     8761 cccagacctc tctgccgtcg ggcgagatgt cgatggcctc gggctgcttg ccgaccggga
     8821 cgtgcgtgac gcgcggcggg gggccgggct gcgagggctt gtcgagttcg atgacggagg
     8881 ccgtgtccga gccgatgttg gccgtgtaga tggtcttccg cgtcgggtgg aagacgagca
     8941 tgtgcgtgcc cttctcgccg aggcccatca cccagtcaac cttgtcgctc gccgggtcgt
     9001 agcgcgcgac ggcgcggttc acttccgccg tgaagtaaat cttgccctct cgctcgacca
     9061 gcccgtgcgg gcgccacagc gcgccgagat cgacgcgctt ggtctccttg cgcgccgcaa
     9121 ggtcgatgat ggagatgctg ttgcccggtt tctgcccgcc gtagttcgag acgtaagccg
     9181 tgcgcccgtc cgccgaagtc acgacctcgt gcggcccttc gcccaccgcg acgcgcccga
     9241 ccgcctccat cttcaccggg tcgatgatcg tgaccgaggc ttcggccttg ttcagcacga
     9301 gcaggtaaga ggcggacgag gcagcgggcg aagccgcctg cccctgctgt gcgcgtgcgg
     9361 agggcgcgtg cgacgggtcg gtgattatca cgagcacgag cgcggcggcg gcgaagagc
//
LOCUS       DBNGOH010000062         7105 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3125742, whole genome shotgun sequence.
ACCESSION   DBNGOH010000062 DBNGOH010000000
VERSION     DBNGOH010000062.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7105)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7105)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7105
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3125742"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1942)
                     /locus_tag="AC159P_01210"
     CDS             complement(<1..1942)
                     /locus_tag="AC159P_01210"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012766.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HIR3694096.1"
                     /translation="MKTAHSLKRLVQAAVIIILVALVVSGVVAPRQQTATATQAAALR
                     AAPPAPVFGNEERVAELAERRRKVAEKIGPKSVLVMFSGEPRVYTNDVSYEFRMENNL
                     FYLTHLKQQSATLVLMPGAEGPKEILFIPRRNPAAETWTGHMYSPEEARRISGVNEIW
                     EAKEFEPFVRALRTRRPYRPQGTGVLYTSVSQAEGAAQPITPPVPKSDQTPEAASAAT
                     PARPVSGNTPANLVTQNATPAAAAPASTTPAAAGQAVAAPSSADPNAPALYLLLPSRE
                     GNREFTQEQRFAAEWAKEPAELKTRSAWPIFVELRMRKSPSELRLLQHAIDISIEGHQ
                     RAQAVAPSAKWEYEVEAELEYTYKRRNADHWGYPNIVGCGPNATTLHYEESQGQVKDG
                     DLILIDSGAEYEHYTADVTRTFPARGKFSAPQKEIYDIVLAAQEASFRASKPGATIAD
                     VHNASVEVIKDGLLRLGLITDRNTDQFRVWFMHGTSHWLGMNVHDVGVRGIKLDAGMV
                     YTVEPGIYIRLDALDYLPDTPENKKFVAAVRPAFEKYKGIGVRIEDDILITPDGYKNL
                     SAALPRTAADIEAFMARAQREIRSDLFDGEGWMKDRALLHGPRVLGTAGFDGEFHWSS
                     MNLPHDFTGATLRRGHTFVGSPA"
     gene            complement(2180..3946)
                     /gene="ggt"
                     /locus_tag="AC159P_01215"
     CDS             complement(2180..3946)
                     /gene="ggt"
                     /locus_tag="AC159P_01215"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036374 - glutathione gamma-glutamate
                     hydrolase [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HIR3694097.1"
                     /translation="MRRIITTFFVACLLVFAPASSLTNGVARFDATAAAAPQAARQPV
                     RAVRGMVASDSRIASEVGVSVLKRGGNAVDAAVAVALALAVVYPEAGNLGGGGFMLMR
                     LGDGRTAAIDYREMAPAAAHRNLFLDAKGELVQGEGSSTRGYRASGVPGTVAGLSLAL
                     KKYGSGRLTWSQLVEPARKLAAEGFTLTYSLAKTFRDNSKEMLQYPESRRVFLKDGKF
                     FAEGEVFRQPELAATLARLQAHGPREFYEGQTARLIAADMKKHGGLMTEDDLRAYAPR
                     EREPLRSTYRGHEVISMPPPSSGGVALIEMLNILEGYDLRAMGWSSSETFHVMTEAMR
                     RAYADRAAYLADPDHAKVPAAGLTDKSYAARMRGTINPERASTSAEIKGGRPAGSEPS
                     ETTHFSVVDAEGNVVANTYTINDLFGSGVTAKGTGVLLNDEMDDFTARPGTPNRWGAI
                     QGEQNRVEPRKRPLSSMTPTIVMRKDGTVWFAVGARGGTRIINAVLQIVVNVVDFEMN
                     IQQALDAPRIHHQWLPDVLLFEAFGMSPDTRRVLEGRGHKVERASDPLHIAAANAIMI
                     EEKTGARLGATDPRKDGGAVGY"
     gene            complement(3982..4476)
                     /locus_tag="AC159P_01220"
     CDS             complement(3982..4476)
                     /locus_tag="AC159P_01220"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012427758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3694098.1"
                     /translation="MMMTTAFQLTRAETAEEFAVARELFEEYAAALDVDLCFQNFERE
                     LNNLAAEYGPPEGRLLLASAAGEPVGCVGVRKLADGVCEMKRLYVRPRARGTGLGRLL
                     ANEIVGAARELGYGSMRLDTLPSMKEAVPLYRSIGFKETEAYRYNPVEGTIYMELDLS
                     EERG"
     gene            complement(4493..5668)
                     /locus_tag="AC159P_01225"
     CDS             complement(4493..5668)
                     /locus_tag="AC159P_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HIR3694099.1"
                     /translation="MTFESEHAQTDRFVEHFRERREQVLALTRSLVETESPSGDGEGS
                     RAVADLLAGEARGLGLLTSIERIAAPDGYGEHLRIRVGVDDAGVDKGVLVLGHTDTVH
                     PRGTLAERPWREAEGRIYGPGVFDMKASCALALEALRARGQSAAGRARPVVLLLTCDE
                     EAGSMSGRALVEEEARRAECVLVLEPPAPGGRVKTARKGTGYFRLAVEGVAAHAGLEP
                     EKGASAVLEIARQIERLHALNDAARGTTVNVGVVCGGTRSNVVAAEARAEVDVRFGTL
                     EEARRIEEAIRALHPFDARTSLKVEGGVNRPPLERTPAVARLYEHARRLASAVGFELG
                     EASVGGASDGNFAAACGVPVLDGLGVEGDGAHAAHEHILADCLVSRGALLASLLATL"
     gene            complement(5743..6873)
                     /gene="menC"
                     /locus_tag="AC159P_01230"
     CDS             complement(5743..6873)
                     /gene="menC"
                     /locus_tag="AC159P_01230"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013567968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HIR3694100.1"
                     /translation="MKITSIEMREIRLRLVHFFETSFGRTIERRILLVRVRDAEGGEG
                     WGECTAGEGPFYCEEWIDSAWEVTRSFLAPMVAGREVERAAEVFGLMARVRGNRMAKG
                     AVETACWDLEARSLGVPLWRHLGGVRGEIPCGVSIGIQDTPEALLEKIEKELAAGYQR
                     IKIKIKPGWDREIVETVRKRFPQVPLMVDANSAYTLDDAPLFRALDEFDLMMVEQPLA
                     FDDREDHAKLQRQIKTPVCLDESVRSAEDARKAIEAGACRIVNVKLGRVGGHAEARRV
                     EQVCREGGVPVWCGGMLESGVGRAHNIAMATLEGFTLPGDVSASARYWEEDIVEPPVT
                     VTPRGTIIAPESPGLGYEIRRERIESLTVRKYEHSSDRSPGG"
BASE COUNT         1050 a         2517 c         2332 g         1206 t
ORIGIN      
        1 cggcgggcga gccgacgaag gtgtggccgc ggcggagggt ggcgcccgtg aagtcgtgcg
       61 ggaggttcat ggacgaccag tggaattcgc cgtcgaagcc cgccgtcccc agcacccgag
      121 ggccgtgcag aagggcgcgg tctttcatcc agccttcgcc gtcgaagagg tcggagcgga
      181 tttcgcgctg ggcgcgggcc atgaaggctt cgatgtcggc ggcggtgcgc gggagcgcgg
      241 cggagaggtt cttgtagccg tcgggggtga tgaggatgtc gtcctcgatg cggacgccga
      301 tgcctttgta cttctcgaag gcgggccgga cggcggcgac gaacttcttg ttctcgggcg
      361 tgtcgggcag gtagtcgagc gcgtcgagcc ggatgtagat gcccggctcg accgtgtaga
      421 ccatcccggc gtcgagcttg atgccgcgca cacccacgtc gtgcacgttc atcccgagcc
      481 agtgcgaggt gccgtgcatg aaccagacgc ggaactgatc cgtgttgcga tctgtgatga
      541 ggccgaggcg gagcagcccg tccttgatca cctcgaccga ggcgttgtgt acgtcggcga
      601 ttgttgcgcc cggcttgctg gcgcggaagg aagcctcctg cgcggcgagc acgatgtcgt
      661 aaatctcttt ctgcggcgcg ctgaacttgc cgcgcgccgg gaaggtgcgc gtcacgtccg
      721 ccgtatagtg ctcgtactcg gcgcccgagt cgatcaggat gaggtcgccg tctttgacct
      781 gcccttgcga ctcttcgtag tggagcgtcg tcgcgttggg gccgcagccg acgatattcg
      841 ggtagcccca gtggtcggcg ttgcgccgct tgtaggtgta ctccagctcg gcctcgacct
      901 cgtactccca cttcgcgctc ggcgcgacgg cctgcgcgcg ctggtgcccc tcgatgctga
      961 tgtcgatggc gtgctgcaag aggcgcagct ccgaaggcga cttgcgcatc cgtagctcga
     1021 cgaagatggg ccacgccgaa cgtgtcttca gctcggcagg ctctttcgcc cactccgccg
     1081 cgaaccgctg ctcctgcgtg aactcgcggt tgccctcgcg cgagggcagc agcaaataga
     1141 gcgcgggtgc gttcgggtcg gcggatgagg gggcggcgac cgcttgcccg gcggcggcag
     1201 gcgtcgtcga agccggcgcc gccgcggcgg gggtcgcgtt ctgcgtgacg aggttggcgg
     1261 gcgtgttgcc gctgacgggg cgcgcgggcg tcgcggcgga cgcggcctcg ggcgtctggt
     1321 cggacttggg cacgggcggc gtgatgggct gcgccgcgcc ctcggcctgc gagacggatg
     1381 tgtagagcac gcccgtgccc tgcgggcggt agggccggcg cgtgcgcaag gcgcgcacga
     1441 acggctcgaa ctctttggcc tcccagattt cgttgacgcc cgagatgcgg cgcgcctctt
     1501 cggggctgta catgtggccc gtccaagtct cagccgcagg gttgcggcgc gggatgaaga
     1561 gaatctcctt cggcccctcc gcacccggca tcaggacgag cgtcgcgctc tgctgcttga
     1621 ggtgcgtcag gtagaagagg ttgttctcca tcctgaactc gtagctcacg tcgttcgtgt
     1681 agacgcgcgg ctcgccgctg aacatgacga ggacggactt cgggccgatc ttctcggcca
     1741 ccttgcggcg ccgttcggcg agttcggcca cgcgctcttc gttcccgaag acgggcgcgg
     1801 gcggcgccgc gcgcagagca gcagcctgcg tcgccgtggc cgtctgctga cgcggcgcga
     1861 ccacacccga caccacgagg gcgacgagga taatgatgac ggctgcctgc acgagccgtt
     1921 tcaaagagtg tgctgttttc aattccttac tcctttaaaa aaattccacc tcgcgtcgcc
     1981 gacgttgcgg ctctgccgcc cgatgtccgg tgaggctcgg cctcaccgga acgcccccgc
     2041 ctcgcgcggc ggcgtagccg cccaattcag aaagaagtta ctgcccggaa aggcagagcc
     2101 tttccgcaca tcaggcggcc gagccgccaa caaacacacc catgtggtcg cttgaaatga
     2161 cacacaacca tgtatggcct caataaccga ccgcgccgcc gtccttgcgc gggtcggtcg
     2221 cgccgagccg cgcgccggtc ttctcctcga tcatgatcgc gttggcggcg gcgatgtgca
     2281 gcgggtctga cgcgcgctcg accttgtgtc cgcgcccttc gagcacgcgc cgcgtgtcgg
     2341 gcgacatccc gaaagcctcg aagagcagca cgtccggcag ccattggtga tggatgcgcg
     2401 gcgcgtccag agcctgctgg atgttcatct cgaagtccac gacgttgacg acgatttgca
     2461 gcacggcgtt gatgatgcgc gtgccgccgc gcgcgccgac ggcgaaccag acggtgccgt
     2521 ccttgcgcat gacgatggtc ggcgtcatag aggagagcgg gcgcttgcgc ggctcgacgc
     2581 ggttctgctc gccctgaatc gcgccccagc ggttcggcgt gccgggccgc gccgtgaagt
     2641 cgtccatctc gtcgttgagc agcacgcccg tccccttcgc cgtcacgccc gaaccgaaga
     2701 ggtcgttgat cgtgtaggtg ttggcgacga cgttgccttc ggcgtccacg accgagaaat
     2761 gcgtcgtctc ggacggctcg ctgcccgcgg gccgcccgcc tttgatctcg gcgctcgtcg
     2821 aggcgcgctc cgggttgatc gtgccgcgca tgcgcgccgc gtaactcttg tcggtgagac
     2881 ccgcggcggg caccttcgcg tggtcggggt ctgcgaggta ggcggcgcgg tctgcgtagg
     2941 cgcggcgcat cgcctcggtc atcacgtgga acgtctcgga ggaagaccaa cccatcgccc
     3001 tgaggtcgta gccttcgagg atgttcagca tctcgatcag cgcgaccccg cccgaagaag
     3061 gcggcggcat cgagatcact tcgtggccgc ggtaggtcga gcgcaaaggc tcgcgctcgc
     3121 gcggcgcgta cgcgcgcagg tcgtcttccg tcatcaggcc gccgtgcttc ttcatgtcgg
     3181 cggcgatgag tcgcgcggtc tgcccctcgt agaactcgcg cgggccgtgc gcctgcaagc
     3241 gcgcgagcgt ggcggccagc tcgggctgcc tgaacacctc gccctccgcg aagaacttgc
     3301 cgtccttcaa aaagacgcga cggctctccg ggtactgcaa catctctttg ctgttgtcgc
     3361 ggaaggtctt cgccagcgag tacgtcaggg tgaagccttc ggcggccagc ttgcgcgcag
     3421 gctcgacgag ctgcgaccag gtgagccgac ccgacccgta cttcttcaga gcgagcgaga
     3481 ggccggccac cgtgcccggc acgcccgacg cgcggtagcc gcgcgtcgag gagccttcgc
     3541 cctgcacaag ctcgcccttc gcgtcgagga agaggttcct gtgcgcggcg gcgggcgcca
     3601 tttcgcggta gtcgatcgcc gccgtccgcc cgtcgccgag acgcatcagc atgaagccgc
     3661 cgccgcccag attccccgcc tccgggtaga cgaccgcgag cgccagcgcc accgcgaccg
     3721 ccgcgtctac cgcgttaccc ccgcgcttca agacgctcac gccgacctcg gacgcgatgc
     3781 ggctgtccga cgccaccatg ccccgcaccg cgcgcacggg ctggcgcgcg gcctgcggcg
     3841 cggcggcggc ggtagcgtcg aagcgcgcga ccccgttcgt gagcgacgag gcgggcgcga
     3901 agacgaggag gcaggcgacg aagaaagtcg tgatgatgcg tctcatggtc attcgcgttc
     3961 gattcctgta gaccgcttcg tttaaccgcg ttcttcggac aaatccagct ccatgtaaat
     4021 cgtgccctcg acgggattgt agcggtaagc ctccgtttcc ttaaacccaa tcgaacggta
     4081 gagcggcacc gcctccttca tcgagggcag cgtgtccagc cgcatcgagc cgtagcccaa
     4141 ctccctcgcg gccccaacaa tctcgttcgc cagcaagcgg cccagccccg tcccgcgcgc
     4201 gcgcggtctc acgtacagac gtttcatctc gcacacgccg tcggcaagtt tcctcacgcc
     4261 gacgcagccc acaggctcgc ccgccgccga agccagcagc agacgcccct ccggcgggcc
     4321 gtactccgcc gcgaggttgt tcagctcgcg ctcgaaattc tgaaagcaca gatcgacgtc
     4381 gagcgccgct gcgtactctt cgaacaactc gcgcgcaacg gcgaactctt ccgccgtctc
     4441 ggcccgtgtc aattgaaagg cagtcgtcat catcatggcg ttgcgtttct cttcagagcg
     4501 tcgccagcag ggacgcgagc agcgcgccgc ggctgacgag acaatcggcg aggatgtgct
     4561 cgtgggcggc gtgcgcgccg tcgccttcga caccgaggcc gtcgaggacg ggcacgccgc
     4621 aggcggcggc gaagttgccg tcggacgcgc cgcccacgct ggcctcgccc agctcgaagc
     4681 cgaccgcgga tgcgagccgc cgcgcgtgct cgtagagtcg cgcgaccgcc ggagtcctct
     4741 ccagcggcgg gcggttcacg ccgccttcca ccttcagact cgtccgcgcg tcgaacgggt
     4801 gcagggcgcg aatcgcttct tcgatgcgcc gcgcctcttc gagcgtgccg aagcgcacgt
     4861 ccacctcggc gcgcgcctcc gccgcgacga cgttcgagcg cgtcccgccg cacaccacgc
     4921 cgacgttcac cgtcgtcccg cgcgccgcgt cgttcagcgc gtgcagccgc tcgatctgcc
     4981 gcgcgatttc gagcaccgcg ctcgcgccct tctccggctc aagccccgcg tgcgcggcca
     5041 cgccctcgac cgccagacgg aagtaacccg tccccttgcg cgccgtcttc acgcgaccgc
     5101 ccggcgcggg cggctcaagc acgagcacgc actccgcgcg ccgcgcctcc tcttcgacga
     5161 gcgcgcgccc gctcatgctc cccgcctcct cgtcgcacgt caacaacaac acgacgggcc
     5221 gcgccctgcc cgccgccgac tggccgcgcg cgcgcaacgc ttcgagcgcc agcgcgcagc
     5281 tcgccttcat gtcgaagacg cccggcccgt agattctccc ttcggcctcg cgccacggcc
     5341 tctccgccag cgtgccccgc gggtgaaccg tgtccgtgtg gccgaggacg aggacgcctt
     5401 tatcgacacc cgcgtcgtca accccgactc gaatgcgaag gtgctcgccg tagccgtccg
     5461 gcgccgcgat gcgctcgatc gaagtgagga gaccgagccc ccgagcttca ccggcaagta
     5521 gatcggcgac ggcgcgactc ccctcgccgt cgcccgaagg cgactcggtc tcgaccagcg
     5581 agcgtgtcag cgcaaggact tgctcgcgcc tctctctgaa gtgctcgacg aagcggtcgg
     5641 tttgtgcgtg ctcggactcg aatgtcatgc gggccgtcgg attgtttgta tcggtggttc
     5701 ggatgcgaaa cgtgtgatga cgtgaagcga aacttcgaag ccctagccgc ccggactcct
     5761 gtcggacgag tgctcgtact tgcgcaccgt cagcgactcg atgcgctcgc gcctgatctc
     5821 gtagccgagg cccggcgact ccggcgcgat gatcgtcccg cgcggcgtca cggtcacggg
     5881 cggctcgacg atgtcctcct cccagtagcg cgcgctggcc gacacgtccc ccggcagcgt
     5941 gaacccttcg agcgtcgcca tcgcgatgtt gtgcgcgcgc ccgacgcccg attcgagcat
     6001 cccgccgcac cacacgggta cgccgccctc gcggcagacc tgctcgacgc gccgcgcctc
     6061 ggcgtgcccg ccgacgcggc ccagcttcac gttgacgatg cggcacgcgc ccgcctcgat
     6121 ggccttgcgc gcgtcctccg ccgagcgcac cgactcgtcg agacagacgg gcgttttgat
     6181 ttgacgttgc agcttcgcgt gatcctcgcg gtcgtcgaag gcgagcggct gctcgaccat
     6241 catcaagtcg aactcgtcga gcgcgcggaa gagcggcgcg tcgtcgagcg tgtaggccga
     6301 gttcgcgtcc accatcaagg gaacttgggg gaaacgcttc cttacggtct cgacgatctc
     6361 acgatcccat cccggcttga tcttgatctt gatgcgttga taaccggcgg ccagctcctt
     6421 ctcgatcttt tcgagcagcg cctcgggcgt gtcctgtatg ccgatggaga cgccgcaggg
     6481 gatttcgccg cgcacgccgc cgaggtgtct ccacagcggc acgcccaagc ttcgcgcctc
     6541 caggtcccag cacgccgtct cgaccgcgcc cttcgccatg cggttgccgc gcacgcgcgc
     6601 catcagcccg aagacctccg ccgcccgctc gacttccctg cccgcgacca tcggcgcgag
     6661 gaaactccgc gtcacctccc acgcgctgtc gatccactcc tcgcagtaga aaggcccctc
     6721 gcccgccgtg cactcgcccc agccctcgcc gccttcggcg tcgcgcacgc gcacgagcag
     6781 gatgcggcgc tcgatggtgc gcccgaagct tgtctcgaag aagtggacga gccgcagccg
     6841 aatctcgcgc atctcgatgg atgttatttt caccgacgaa tgaactccga ttttttacgg
     6901 gcgagccgcg gctattatac cgaccgcgaa cgcctcttca caaagaacgg cgacccgaag
     6961 gccgccgtct tgaaagtcac cgcccggatc gatttcgctt caacccttca cccctccgac
     7021 gcgcgactca ctccgccgcc cgcgcggcct gctccggcgt cagcgcgggg ccgaggttct
     7081 cggtcgtcgc catctcttcg ccttc
//
LOCUS       DBNGOH010000063         3520 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3309537, whole genome shotgun sequence.
ACCESSION   DBNGOH010000063 DBNGOH010000000
VERSION     DBNGOH010000063.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3520)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3520)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3520
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3309537"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(255..1733)
                     /locus_tag="AC159P_01235"
     CDS             complement(255..1733)
                     /locus_tag="AC159P_01235"
                     /inference="COORDINATES: protein motif:HMM:NF012485.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II and III secretion system protein family
                     protein"
                     /protein_id="HIR3694101.1"
                     /translation="MQKRSLVARLVVLVCAALAFAPSVLAQQHVSGSFENPAQEPTQI
                     NVLVGQSRVINFDKQIGRFSVSNPEVAEAVLVAPDQVLVNGKSFGQVNFIAWEKEGKQ
                     FMVFDVFVRANLSLIDSQIRALFPKDDVRLSQANGSVVLSGTVASVKTSEQVEAVVKA
                     AGFNTVNMLQSPVMAKAQVQLQVRVAEVSRTKLRELGGSFAYESRPGVGGTIGSGGGP
                     FTVSKIDQGSIFGSTIGSALNLFVMGGNTYNWIHALKTQGALRSLAEPNLIAMDGQQA
                     SFLAGGEFPVPIVQGGGDRNNVTIVFKEYGVRLNFKPTIIDENHIRLELEPEVSTIDF
                     ANGVRFDGFVIPALKTRRAKTGVELRDGQSFALAGLLDNSETRSLSKIPILGDIPVLG
                     TLFSSKSFQKTETELMFIVTAELVKPVNPDDLPQLRGVDGIKNGSPLGVEPKGEGITG
                     DHGYATGEKSSSGTTAPAAPQPEAQPKKTTAEGAVVVSKKMQ"
     gene            complement(1848..2777)
                     /gene="cpaB"
                     /locus_tag="AC159P_01240"
     CDS             complement(1848..2777)
                     /gene="cpaB"
                     /locus_tag="AC159P_01240"
                     /inference="COORDINATES: protein motif:HMM:TIGR03177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HIR3694102.1"
                     /translation="MRNKRLVVVLVGAALFGLVAAVSVSRYLSNAQANPRTNTVVVAK
                     VDIPLGTKIVAEQLATAQFPASAIPDGTFDSVEKMVGRVAVVNVAAREPITDFKLAPE
                     GSTGGLSAVIPEGYRAMTVKVDDVMGVSGFLQPGVMVDVLTVIEPPDDGSRKDPISKI
                     VLQNVKVLASGQNLDKPKNEREADSVKAVTLQVTPEQAEKLALASTEGKLRLVMRNSV
                     DQGDEQTTGVNTRTLLTGERAMPAPEPGSLKSEQPKPVAPAPAPVMHRQPRAAKPREE
                     PPAPAAPAPAPPAPPRPSVEMIQGAKKSTVEFP"
     gene            complement(2829..3356)
                     /locus_tag="AC159P_01245"
     CDS             complement(2829..3356)
                     /locus_tag="AC159P_01245"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF013632.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HIR3694103.1"
                     /translation="MSNPLVIASAALLIPLAGLIAYYDVIYRRIPNWLVLITLITGLI
                     LNILFGGIDGALASLGGCLLAFVAMLVLHVFGAMGAGDVKLFAAVGSVIGLQYIMPTF
                     VVVLLTGGVLGVITMVHAGTVVSTMNRVLHIFFGLLPGWPMPRFAVPADRRHTIPYGV
                     AITLGSLISLFAFRA"
BASE COUNT          564 a         1152 c         1159 g          645 t
ORIGIN      
        1 aagtgactga tgtctacgca taaaccggtg gcgagcagga acgtgagcat gccgatggcg
       61 ctcatcgcca ggaccgaacc gcgctccccg ttccccttcc gctcggagtt aatctgcttc
      121 atcttccccc tccttccacg ctatcggtac gctctgccgt ccgccgcccc cctctacggc
      181 ccggcgcggc ggagaatgct ctccgccgcc cgggcctgtt cgagcgccgg gatggcgcgc
      241 ggcgcgtcag gctgttactg catcttcttg gagaccacca ctgccccctc agccgtcgtc
      301 ttcttcggct gcgcttccgg ttgcggcgcc gcgggcgccg tcgtccccga gctgctcttt
      361 tcgcccgtcg cgtagccgtg gtcgcccgtg atgccctcac ccttgggttc gacgccgagc
      421 ggcgagccgt tcttgatgcc gtccacgccc cgtagctgcg gcaggtcgtc ggggttgaca
      481 ggcttcacga gttccgccgt gacgatgaac atcagctcgg tctcggtctt ctggaacgac
      541 ttcgagctga agagcgtgcc gagcaccggg atgtcgccga ggatggggat cttcgagagc
      601 gagcgcgtct cgctattgtc gagcagcccg gcgagggcga agctctggcc gtcgcgcagc
      661 tcgacgcccg tcttggcgcg acgcgtcttc agcgccggga tcacgaagcc gtcgaacctc
      721 acgccgttgg cgaagtcgat ggtcgagacc tcgggttcga gttcgaggcg gatgtggttc
      781 tcgtcgatga tggtcggctt gaagttgagg cggacgccgt actccttaaa gacgatggtg
      841 acgttgttgc ggtcgccgcc gccctggaca atcgggacgg ggaactcgcc cccggcgagg
      901 aagctcgcct gctggccgtc catcgcgatc aggttcggct cggccaagct gcgcagggcg
      961 ccctgcgtct tcagggcgtg aatccagttg taggtgttgc cgcccatcac gaagaggttc
     1021 agggcgctgc cgatggtgct cccgaagatg ctgccctggt cgatcttact gaccgtgaac
     1081 gggccgccgc ccgagccgat ggtgccgccg acgccggggc gcgactcgta ggcgaagctc
     1141 ccgcccagct cgcgcagctt cgtgcggctg acctcggcga cgcgcacctg gagctggacc
     1201 tgcgccttcg ccatcaccgg cgactggagc atgttgaccg tgttgaagcc cgcggccttg
     1261 acgaccgcct cgacctgctc ggaggtcttc acgctggcga ccgtgcccga gagcacgacc
     1321 gagccgttgg cctggctcag gcggacgtcg tccttcggga agagcgcgcg aatctgcgag
     1381 tcgatcagcg agaggttggc ccgcacgaac acgtcgaaga ccatgaactg cttgccctct
     1441 ttctcccacg cgatgaagtt gacctgcccg aacgacttgc cgttgacgag cacctggtcg
     1501 ggggcgacca gcaccgcctc ggccacctcc gggttcgaga ccgagaagcg cccgatctgc
     1561 ttgtcgaagt tgatgacgcg cgactgcccg accagaacgt tgatctgggt gggttcctgc
     1621 gccgggttct cgaaagaacc gctgacgtgt tgctgtgcga gcacgctcgg cgcgaaggcg
     1681 agcgccgcgc agacgagcac taccaggcgt gcgacgagag aacgtttctg catgatcatt
     1741 tgctccttca gataaagctg ccagtcatta gcttcaagcg atagctttca gtgatcagcc
     1801 cgcgcaatcc ttctcggccc tttgccgggg gctgacggct gacagcttta cggaaattcg
     1861 acggtgctct tcttcgcacc ctggatcatc tcgacggaag ggcgcggcgg cgcgggcggc
     1921 gcgggcgcgg gcgcggcggg cgcgggcggc tcctcgcggg gcttggcggc gcggggctgc
     1981 cggtgcatga cgggcgccgg cgccggggcg accggcttcg gctgctcgct cttcagcgag
     2041 ccgggctccg gggcgggcat cgcgcgctcg ccggtcagca gcgtccgggt gttcaccccc
     2101 gtggtctgct cgtcgccctg gtcaaccgag ttacgcatga cgaggcggag tttcccttcc
     2161 gtggaagcca gcgcgagctt ctcggcctgc tccggcgtga cctgcagcgt gaccgctttg
     2221 acgctatccg cctcgcgctc gttcttcggc ttgtcgaggt tctggccgct ggcgagcacc
     2281 ttcacgttct ggaggacgat cttcgagatc gggtctttcc tggaaccgtc gtcgggcggc
     2341 tcgatgacgg tcagcacgtc aaccatcacg ccgggctgga ggaagccgga gacgcccatc
     2401 acgtcatcga ccttcaccgt catcgcgcgg tagccttcgg ggatgaccgc cgagaggccg
     2461 cctgtcgagc cttccggcgc gagtttgaag tccgtgatag gttcgcgcgc cgcgacgttg
     2521 acgaccgcga cacgccccac catcttctcg acgctgtcga aggtgccatc ggggatcgcg
     2581 ctcgcgggga actgcgccgt cgccagctgc tcggcgacga tcttcgtgcc cagcgggatg
     2641 tcaactttgg ccaccaccac cgtgttcgtc cgcgggttgg cctgcgcgtt agacagatac
     2701 cggctcaccg agacggcggc gacgaggccg aacagcgcgg cgcccacgag cacgacgaca
     2761 agacgtttgt ttcgcatgat tctcgttctc caaattgggg gaaatactta cggcccgccc
     2821 gtcggctctc aggcccggaa ggcgaagagc gaaatgagac tccccagcgt gatggcaacc
     2881 ccgtagggaa tcgtgtggcg gcggtcagcc gggacggcga agcgaggcat cggccacccc
     2941 ggcagcaaac cgaagaagat atgcaggacg cggttcatgg ttgatacaac cgtgcccgcg
     3001 tgcaccatcg tgatgactcc cagcaccccg cccgtcagca acaccaccac gaaggtcggc
     3061 atgatgtact gcaggccgat gactgatccg acggctgcga acagcttcac gtcgcccgcg
     3121 cccatcgccc cgaaaacatg cagcaccagc atcgccacga aggcgagcag gcatccgccc
     3181 aggctggcga gcgcgccgtc tatgccgccg aagaggatat tgagtatgag tccggtaatc
     3241 agcgtaatga ggacgagcca gttcggtatc cgcctgtaga tcacgtcgta gtaagcgatg
     3301 agccccgcca acgggatcag cagcgcggca ctggcgatga cgagggggtt agacatcact
     3361 tgtgattcct cgggtgtctc gtggacttga tctcgcgttt cttaaattcg cttgtaaatg
     3421 caacgcgttc gcccgcgccc tttgctactc gcgcacggct acagtggggt tgaggcggta
     3481 actcgggggg gccgccggcg cagggtaggc taggccggca
//
LOCUS       DBNGOH010000064         1629 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4409228, whole genome shotgun sequence.
ACCESSION   DBNGOH010000064 DBNGOH010000000
VERSION     DBNGOH010000064.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1629)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1629)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1629
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4409228"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            264..1526
                     /locus_tag="AC159P_01250"
     CDS             264..1526
                     /locus_tag="AC159P_01250"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014081.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR3694104.1"
                     /translation="MKRLFRLAVFFLAVVGVDAVAQVTAIRAGKLVVPETGATLTNRI
                     ILVEGTRIKAVGPEVPIPPGATVIDLSRQTVLPGLFACHEHMAFLINAKGRERGSLFF
                     YDLTHTNAERAIHAVVNSRDMLDAGFTTIRDIGNDGNYVSTDLRRAIDRGLIPGPTVI
                     NSGRIIAPYGGQYQLQPERRDLGEPEYLYADTRDEMRKAIRQNAHFGARVIKIVVDDQ
                     PYIYSADDIRYMVAEAAAAGLKLAAHCVTEQGARNAAEAGVASIEHGFEMSNEALELA
                     KKHNVVLVGTDFTEKAWLAYEMPVELAKRFHARSVDRLRRAHRIGVTLAFGSDLIFYA
                     PDETRGTWTMSQIETFVEAGIPSKVILQAMTTNAARLLGVEKVRGAISPGQFADIIAT
                     PENPLDNISTLKRVGFVMKNGKVFKQAK"
BASE COUNT          294 a          553 c          518 g          264 t
ORIGIN      
        1 gcggcgaccc gcgcgggtcg ccgccccttc tttttttagc ccctgccaca gggacttcga
       61 gagtcggcca cgtcgtctcg cgcggagact tccgatagcc tctcccggtc ttctcgggtt
      121 agactgctac ttctcaacat cagtgtcgag tctgcttgtc ggtcacgctc catgcgcgtc
      181 gccgttgacg cgtgacaatc gcacgccccg gcgcccgtcg ccgttaattc cgcggaggca
      241 aaatggcaat cgaaaggaca aagatgaaaa gacttttccg gctcgccgtc ttcttcctcg
      301 ccgtcgtcgg cgttgacgcg gtcgcgcagg tgacggccat cagggccggc aagctggtcg
      361 tgccggagac gggcgcgacg ttgacgaatc ggatcatcct cgtcgagggc acgaggatca
      421 aggcggtcgg tcccgaagtg ccgatcccgc ccggcgcgac cgtcatcgac ctctcgcgcc
      481 agaccgtgct gcccggtctc ttcgcctgcc acgagcacat ggccttcctc atcaacgcga
      541 aggggcgcga gcgcgggtcg ctcttctttt acgacctgac gcacaccaac gccgagcgcg
      601 ccatccacgc cgtcgtcaac tcgcgcgaca tgctcgacgc gggcttcacc accatccgcg
      661 acatcggcaa cgacggcaac tacgtctcca cagacttgcg gcgcgcgata gaccgcgggc
      721 tgatacccgg cccgaccgtc atcaactcgg ggcggatcat cgccccctac ggcgggcagt
      781 accagctcca gcccgaacgg cgcgacctcg gggagccgga gtacctctac gccgacacgc
      841 gcgacgagat gcgcaaggcc atccgccaga acgcgcactt cggcgcgcgc gtcatcaaga
      901 tcgtcgtgga cgaccagccc tacatctatt cggcggacga catccgttac atggtcgcgg
      961 aggccgcggc ggcggggctg aaactcgcgg cccactgcgt gaccgagcag ggcgcgcgca
     1021 acgccgcaga ggcgggcgtc gcctcgatcg agcacggctt cgagatgtcg aacgaggcgc
     1081 tggagctggc gaagaagcac aacgtcgtgc tcgtcggcac ggacttcact gagaaggcgt
     1141 ggctggctta cgagatgccg gtggagctgg cgaagcggtt ccacgcgcgg agcgtagacc
     1201 gcctgcgccg cgcgcacagg atcggcgtca cgctggcctt cggcagcgac ctcatcttct
     1261 acgcgcccga cgagacgcgc ggcacatgga cgatgtcgca gatcgagacc ttcgtcgaag
     1321 cgggcatccc ttccaaagtc atcttgcagg ccatgaccac caacgcggcg cggctgctcg
     1381 gcgtcgagaa ggtgcgcggc gcgatcagcc ccggccagtt cgcggacatc atcgcgacgc
     1441 ccgagaaccc gctcgacaac atctcgacgc tcaagcgcgt cggcttcgtc atgaagaacg
     1501 ggaaggtgtt caagcaggcg aagtaggccg tcggcggttg ggtgtcagcc agtttcgttt
     1561 caacacgaga gggcacaggg gtcgcacaga gcgcacggag aaagcagttt gtcttctccg
     1621 tgcgctctg
//
LOCUS       DBNGOH010000065         5239 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2759818, whole genome shotgun sequence.
ACCESSION   DBNGOH010000065 DBNGOH010000000
VERSION     DBNGOH010000065.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5239)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5239)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5239
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2759818"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="AC159P_01255"
     CDS             <1..294
                     /locus_tag="AC159P_01255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome D1"
                     /protein_id="HIR3694105.1"
                     /translation="GERHQLRFKVTDAGTNRPAVVKDMGTLVFHAPGWQQRDLARPLG
                     DGVYEISFVPPQEGVYYVYFQAPSLGVRHQHLPFFTLSAAKPDAAPDPPAARP"
     gene            386..1153
                     /locus_tag="AC159P_01260"
     CDS             386..1153
                     /locus_tag="AC159P_01260"
                     /inference="COORDINATES: protein motif:HMM:NF014667.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HIR3694106.1"
                     /translation="MMKTKSMFVLALAALLTGADAAGGGHLTAAAAARQAQTKSRAQG
                     RPARHVCPMHPEVVSAGPGKCPKCGMALRPSKDAPAAVASGGAATADEKGALSAPRIP
                     DIDVYDQSGKRLKFYTDLVKGKTVAVNFIFTTCTTVCPPLSATFRRVQQQLAEAGAAD
                     VQLISVSVDPATDTPERLRDYAAKFKAGPGWTFVTGDKLEIDSLLRALGSAVADKNQH
                     TPSVLVGNDAAGHWTRTYGLSSPAALVKIIRETASHK"
     gene            complement(1171..1521)
                     /locus_tag="AC159P_01265"
     CDS             complement(1171..1521)
                     /locus_tag="AC159P_01265"
                     /inference="COORDINATES: protein motif:HMM:NF024908.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0032196 - transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HIR3694107.1"
                     /translation="MFLCPYCRSAERQVKSGFNRTGTQRIQCQPCRRQYTPLPRPLGW
                     DGETRAAAIRLYLEGNGVRRIGRLLGVNHQSVANWINLLRAQLRAGRAKAGRLAAPAP
                     PSRPKVNHKKANRK"
     gene            1778..>5239
                     /locus_tag="AC159P_01270"
     CDS             1778..>5239
                     /locus_tag="AC159P_01270"
                     /inference="COORDINATES: protein motif:HMM:NF019352.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HIR3694108.1"
                     /translation="MSRSGGRQQSFPSFPTLTLAFLLLSASAVAQTAATRTDATSPAP
                     AAATKAEAAPAPETTPSPEAAPAVAAPAPAEASNVEKDSAKPEAAPAAEAAAPAPSPG
                     PSPSASPGPSPTPAAAAAQQLCTAANTIDVEVIALAQPYLLNRMGASMPHAVIYALAH
                     DVSPFPAPATGYVTNDLKLQSWKRARPIVLRANQGDCLKINLTNLINTNAGGSSTGPV
                     SLHAQGMQAVNSIADDGSYVGANATSLADPLKSKTYTLYAQQEGTFLLYSEGDTNTMG
                     QQLQHGLFGAVNVQPAGAEWYRSQVTREDMALATIRRTAPTSPGNPPGGQPVINYAAR
                     YPANYKDAARACAPILQMAAAEQEVVTVNGVKQCQPKAGGSPRRLYHSDLTAIITGPN
                     AGRFPGTTGVGRPEPACNRAGQPGVNVNPLFCTNPASPDRKQPYREVTVIYHEVTVAV
                     QAFPIFKSGNVQDPANPNDPTKQIPNPAIATTIAGQDLFAINYGTGGIGAEIYGNRIG
                     VGPMANCVDCKFEEFFLTSWAVGDPAMVVDVPANYFVPSPTPSPTPPPCNPNTIKTTD
                     SANNCLIEATRPLVQPITTPPPPQKKATKAFYPDDPSNVYHSYLNDHVKFRILHGGTG
                     VTHVHHQHAHQWLQSPNSSEGSYLDSQFISPGASYTLEMVYNGSGNRNKTLGDSIFHC
                     HFYPHFAAGMWSMWRVHDVFEDGTALDSDGRPLKGARAYPDAEILAGTPIPALVPVPT
                     LPMPLMPSPVFIDNGQIVFGTPLAPDTTGQNVKTNPGYPFFIPGVAGFRPPHPPLDFA
                     PDGAGGFLDGGLPRHVNTGGTVKDQLDFTTDVNNWSKEFVTLNTNQLPEAGTNVEKVA
                     IAFNDKRCNPTYMPDGTLANSFGPANYPECGAAASQAQFIVNGLPNGPQLGAPFADPA
                     VDDQGKAVGKKRLYKAAAIQSDVRFNEKGWHYQQQRLLTLWQDVQPTLNGTRKPEPFF
                     FRANSQDDFIEYWHTNLVPNYYEFDNFQVRTPTDILGQHIHLVKFDVTSSDGAANGWN
                     YEDGTYSPLEVQEAIHAINAAGGINTVGGGKQNLTPKPPPAEIFNCAANPNDARCKPC
                     PPHVEGQPHNLQTQCKSWLGAQTTVQRWYADPFFDNDGQDNTVRTVFTHDHFGPSTHQ
                     QAGLYAGL"
BASE COUNT          945 a         2012 c         1571 g          711 t
ORIGIN      
        1 ggcgagcgcc accagttgcg cttcaaggtc acggacgcgg gcacgaaccg gcccgccgtc
       61 gtgaaggaca tgggcacgct cgtcttccac gcgcccggct ggcagcagcg cgacctcgcc
      121 aggccgctcg gcgatggcgt ctacgagatc agcttcgtgc cgccgcagga gggcgtctac
      181 tacgtctact tccaggcgcc ttcgctcggc gtccgccacc agcatctccc tttcttcacc
      241 ctcagcgccg cgaagccgga cgcggcgccc gacccgccag ccgcgcggcc ctgaacacgg
      301 tgcgaccgcg cgaaggcgtg ccggcgggtt ctgacgacgc gggccgggcc gacggaaaaa
      361 agcgggcttg cttgacggag aaaagatgat gaagacgaag tcgatgttcg tgcttgcgct
      421 cgccgctctg ttgacgggcg cggacgcggc gggcggcggc catctgacgg cggcggcggc
      481 ggcgcgacag gcgcagacga agtctcgggc gcagggccgg cccgcgcgcc acgtctgccc
      541 gatgcacccg gaagtcgttt ccgccgggcc gggcaagtgc cccaagtgcg gcatggctct
      601 gaggccgtcg aaggacgcgc ccgccgccgt cgcttcgggc ggcgcggcca cggcggacga
      661 gaagggcgcg ctctctgcgc cgcgcatccc cgacatcgat gtctacgacc agagcggcaa
      721 gcgcctcaag ttctacaccg acctcgtcaa gggcaagacc gtcgccgtca acttcatctt
      781 caccacctgc accaccgtct gcccgccgct ctcggccacc ttccgccgcg tccagcagca
      841 gctcgccgaa gcgggcgccg ccgacgtgca gctcatctcg gtcagcgtcg atcccgcgac
      901 cgacacgccg gagcggctgc gcgactacgc cgcgaagttc aaggccgggc cgggctggac
      961 gttcgtcacc ggcgataagc tggagatcga ctcgctgctc cgcgcgctcg gcagcgccgt
     1021 cgcggacaaa aaccagcaca cgccgtccgt cctcgtcggc aacgacgcgg cgggccactg
     1081 gacgcgcacc tacggtcttt cctctcccgc cgcgctcgtc aaaatcatca gggagacggc
     1141 gagccacaag tgagccgcct cggcgacgac tcacttgcgg ttcgcctttt tatgattcac
     1201 cttggggcgg gacggcggcg cgggcgcggc cagccgcccg gctttggccc gcccggctct
     1261 gagctgcgcg cggagcaggt tgatccagtt ggcgaccgac tggtggttga cgccgagcag
     1321 ccgcccgatg cggcgcacgc cgttgccttc gaggtagagc cggatggccg ccgcccgcgt
     1381 ctcgccgtcc cagccgagcg ggcggggcag cggcgtgtac tggcggcggc acggctggca
     1441 ctgtatgcgc tgcgtgccgg tgcggttgaa cccggacttc acctgccgct cggcactccg
     1501 gcagtaagga cacagaaaca taccacctct tataccaccg tcaggtcgga tttagacagc
     1561 aggaaaaaaa tggttgacac gggtaatcgt tagaagtagg cttcgcgtgc cataccggac
     1621 acacgttctt tgccccttct tctcactggg ttccaactac ccgccagcga acacatgtct
     1681 ttcgttggcc tcacctcggc tcaagcgatc aagggtcgct ttacttcttt gagcattcac
     1741 cgcccctccg tcggcgaaac ttcaaggaga acaaatcatg tctagaagtg gcggtcgtca
     1801 acaaagcttt ccgagcttcc cgaccttgac actcgccttc ctgctgctct ccgcgtcggc
     1861 ggtcgcccaa accgccgcca cgcgaacgga cgcgacaagc cccgcgccgg ccgccgcgac
     1921 gaaggccgag gccgcgcccg caccggagac gactccttcc cccgaggccg cgcccgccgt
     1981 cgccgcgccc gcgcccgccg aggcgtcgaa cgtcgaaaaa gattccgcga agccggaggc
     2041 cgcgcccgcc gccgaggccg ccgcccccgc cccttcgccc ggcccttcgc cgagcgcttc
     2101 gcccggccct tcgcccacgc ccgccgccgc cgcggcacag caactctgca ctgccgccaa
     2161 caccatcgac gtggaggtga tcgcgctggc gcagccctac ctgctgaacc gcatgggcgc
     2221 ctcgatgccg cacgcggtga tctacgcgct ggcgcacgat gtcagcccct tccccgcgcc
     2281 ggccaccggc tacgtgacca acgacctgaa gctccagtcg tggaagcgcg cgcggcccat
     2341 cgtgctgcgc gccaaccagg gcgactgcct gaagatcaac ctcaccaacc tcatcaacac
     2401 caacgcgggc ggcagctcga ccggccccgt ctcgctccac gcgcagggca tgcaggcggt
     2461 caacagcatc gccgacgacg gctcgtacgt cggggccaac gccaccagcc tcgccgaccc
     2521 gctcaagtcg aagacctaca cgctctacgc gcagcaggag gggacgttcc tgctctacag
     2581 cgaaggtgac accaacacga tggggcagca gctccagcac ggcctcttcg gcgcggtcaa
     2641 cgtccagccc gcgggcgccg agtggtatcg cagtcaggtc acgcgcgagg acatggcgct
     2701 ggcgacgata aggcgcaccg cgccgaccag ccccggcaac ccgcccggcg gccaacctgt
     2761 catcaactac gccgcgcgct accccgcgaa ctacaaggat gcggcccgcg cctgcgcccc
     2821 gatcctgcaa atggccgccg ccgagcagga ggtggtgacg gtgaacggcg tcaagcagtg
     2881 ccagccgaaa gcgggcggct cgccgcgccg tctctaccac agcgacctga cggccatcat
     2941 caccggcccg aacgcggggc gcttccccgg caccacgggc gtcggcaggc ccgagcctgc
     3001 gtgcaaccgc gccggccagc cgggcgtcaa cgtcaacccg ctcttctgca cgaaccccgc
     3061 ctcgcccgac cgcaaacagc cctaccgcga agtcaccgtc atttaccatg aagtgaccgt
     3121 cgccgtgcag gcgttcccca tcttcaagag cgggaacgtg caagacccgg ccaacccgaa
     3181 cgacccgacc aaacagattc ccaacccggc catcgctacc accatcgccg ggcaagacct
     3241 cttcgccatc aactacggga cgggcggcat cggcgccgaa atctacggca accgcatcgg
     3301 cgtcggcccg atggccaact gcgtggactg caagttcgag gagttcttcc tgacctcgtg
     3361 ggcggtcggc gacccggcga tggtcgtgga cgtgccggcc aactacttcg tgccgtcccc
     3421 gacgccgtcc ccgaccccgc cgccctgcaa ccccaacacg atcaagacga ccgacagcgc
     3481 gaacaactgc ctgatcgagg ccacgcgccc gctcgtccag cccatcacca cgccgccgcc
     3541 gccgcagaag aaggcgacga aggctttcta cccggacgac ccctcgaacg tctaccacag
     3601 ctacctcaac gaccacgtca agttccgcat cctccacggc ggcacgggcg tcacacacgt
     3661 ccaccaccag cacgcgcacc agtggctcca gtcgccgaac agctccgagg ggagctacct
     3721 cgacagccag ttcatcagcc ccggcgcgag ctacacgctg gagatggtct acaacgggag
     3781 cggcaaccgc aacaagaccc tgggcgactc gatcttccac tgccacttct acccgcactt
     3841 cgccgccggc atgtggagca tgtggcgcgt ccacgacgtg ttcgaggatg gcaccgcgct
     3901 cgactccgac ggcaggcccc tcaagggcgc gcgcgcgtac ccggacgccg agatcctcgc
     3961 gggcacgccg atcccggccc tcgttcccgt gccgacgctg cccatgccgc tgatgccttc
     4021 gcccgtcttc atcgacaacg ggcagatcgt cttcggcacg ccgctggcgc ccgacacgac
     4081 cggccagaac gtcaagacca accccggcta cccgttcttc atccccggcg tcgcgggctt
     4141 ccgcccgccg cacccgccgc tcgacttcgc cccggacggc gcgggcggct tcctcgacgg
     4201 cggcctgccg cgccacgtca acacgggcgg caccgtcaag gatcagctcg acttcacgac
     4261 cgacgtcaac aactggagca aagagttcgt cacgctcaac acgaaccagt tgcccgaagc
     4321 cgggacgaac gtcgagaagg tggcgatcgc cttcaacgac aagcgctgca acccgaccta
     4381 catgcccgac ggcaccctcg ccaactcctt cggccccgcg aactaccccg agtgcggcgc
     4441 cgccgcctcg caggcgcagt tcatcgtcaa cggcctgccc aacggcccgc agctcggggc
     4501 gccgttcgcc gacccggcag tcgatgacca gggcaaggcc gtcggcaaga agcgcctcta
     4561 caaggcggcg gccatacaat cggacgtgcg cttcaacgag aagggttggc actaccagca
     4621 gcagcgcctg ctgacgctgt ggcaggacgt gcagcccacc ttgaacggga cgcgcaagcc
     4681 cgagccgttc ttcttccgcg ccaactcgca ggacgacttc atcgagtact ggcacacgaa
     4741 cctcgtgccc aactactacg agttcgacaa cttccaggtg cgcaccccga ccgacatcct
     4801 cggccagcac atccacctcg tgaagttcga cgtcacctcg tcggacggcg cggccaacgg
     4861 ctggaactac gaggacggca cctacagccc gctcgaagtg caggaggcca tccacgccat
     4921 caacgccgcg ggtggcatca acaccgtcgg cggcggcaaa cagaacttga cgcccaagcc
     4981 tccgcccgcc gagattttca actgcgcggc caaccccaac gacgcgcgct gcaagccctg
     5041 cccgccgcac gtcgagggcc agccgcacaa cctccagacg cagtgcaaga gctggctcgg
     5101 cgcgcagacc accgtgcagc gctggtacgc cgaccccttc tttgacaacg acgggcagga
     5161 caacacggtg cgcaccgtct tcacgcacga ccacttcggc ccgtccaccc accaacaggc
     5221 gggactctac gccgggctg
//
LOCUS       DBNGOH010000066         3233 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3676387, whole genome shotgun sequence.
ACCESSION   DBNGOH010000066 DBNGOH010000000
VERSION     DBNGOH010000066.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3233)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3233)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3233
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3676387"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="AC159P_01275"
     CDS             <1..186
                     /locus_tag="AC159P_01275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3694109.1"
                     /translation="EYEAMRDVPVVAITAHNTAEFRAAAGAVGCCDYILKPLDFERLF
                     ELLEKLLPQRGAQTATG"
     gene            complement(295..903)
                     /locus_tag="AC159P_01280"
     CDS             complement(295..903)
                     /locus_tag="AC159P_01280"
                     /inference="COORDINATES: protein motif:HMM:NF015147.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LEA type 2 family protein"
                     /protein_id="HIR3694110.1"
                     /translation="MKSMVFGRFRLAATVFVVSLFVTAFGASASAQGRIPVERMGGRL
                     VKIKVGEITLESIDFRDQTARLNLGLDISNGLLPVSLKDFDYALRLNGEETIEGTHAG
                     TLKVGGRNSSRVNLPLTVHLRSIPGTIWSAFRNRGRVQYELDAGFTLPLFITEQRFDQ
                     SFAGEVPLRTLVDAASILRASNMGGSGSRNNERRGGILGWPF"
     gene            complement(1104..2153)
                     /locus_tag="AC159P_01285"
     CDS             complement(1104..2153)
                     /locus_tag="AC159P_01285"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017748514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051075 - S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase activity [Evidence IEA];
                     GO_process: GO:0008616 - tRNA queuosine(34) biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HIR3694111.1"
                     /translation="MSSPFTFTLPPELSAKEPPERRGLARDRVRLMVIERGSERVEHA
                     RFDALGDYLRAGDLLVFNTSRTLPASLEGCGAEGGPCIELRLAEHLPDGAWLALLLCR
                     KGDPFTCGLRAGMNVVFGEELTAAVEGRDEHIPRLWKVRFSKSGHELVDVLYRLGRPV
                     RYEYVSAPWDLDYYQTVYAREPGSAEMPSAGRAFTWRLLFDLKRRGVEAAYLVLHTGL
                     SSYMDDELDARHPASEEQFYVSEQTAARVNRARESGGRIIAVGTTVVRALETVAGRDG
                     RVHARHGYTRLRVTAEHELKAVDGLLTGLHEPQASHLDLLTAFLPAERIREAYEDAVR
                     RGYLWHEFGDLNLIV"
     gene            complement(2263..2511)
                     /locus_tag="AC159P_01290"
     CDS             complement(2263..2511)
                     /locus_tag="AC159P_01290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694112.1"
                     /translation="MADEKDANDTTPEVAARRGASGTDITGGTDKTGRGALAEPDKPA
                     THDTDIEETGDLTGATTTHAGAGGQMDNTGGTGTAPVE"
     gene            2659..>3233
                     /locus_tag="AC159P_01295"
     CDS             2659..>3233
                     /locus_tag="AC159P_01295"
                     /inference="COORDINATES: protein motif:HMM:NF015338.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HIR3694113.1"
                     /translation="MGTFRVVTYNVHKCQGLDRRVRPARIAAVLRELDADVIAMQEVV
                     SVESDARRELHQARFIAEELGYHYCVGENRRHQGGAYGNVILTRLSIEEHCNYDITWR
                     WQEPRGCLRADVRVGEGTTVHVFNAHLGTAFVERRHQGRKLVSDAVLRHSRLHGPRVL
                     LGDFNEWTRGLASRLLSEELLSPDLSRHLRRR"
BASE COUNT          506 a         1094 c         1090 g          543 t
ORIGIN      
        1 gagtatgagg cgatgcgcga cgtgcccgtc gtcgccatca cggcccacaa cacggccgag
       61 ttccgcgccg ccgccggcgc cgtcggctgc tgcgactaca tcctcaagcc gctcgacttc
      121 gaacgcctct tcgaactcct cgaaaaactt ctcccccagc gcggtgccca gaccgccaca
      181 ggataagaag aagggggaag agggtaaacg gggaagaggc gaaaagggag agagcaagcg
      241 cgttgcttgt cttccctttt cgcctctttc ccttttaccc ttttcgcccc tgtcttagaa
      301 gggccagccg aggatgccgc cgcggcgttc gttgttgcga gagccgctgc cgcccatgtt
      361 gctggcgcgc aggatcgagg cggcatcgac gagcgtgcgc agcggcacct cgcccgcgaa
      421 gctctggtcg aagcgctgct cggtgatgaa gagcggcagc gtgaagcccg cgtcgagttc
      481 gtactggacg cgcccgcggt tgcggaacgc tgaccagatc gtgcccggta tcgagcgcag
      541 gtggacggtc agagggaggt tcacgcgcga agagttgcgc ccgccgacct tgagcgtgcc
      601 cgcgtgcgtg ccctcgatgg tctcctcgcc gttcagcctg agcgcgtagt cgaagtcctt
      661 gaggctcacc gggagcaggc cgttcgagat gtcgaggccg aggttgagcc gcgccgtctg
      721 gtcgcggaag tcgatggatt cgagcgtgat ctcgccgacc ttgatcttga cgagccgacc
      781 gcccatgcgt tccacgggga tgcgcccctg cgccgacgcg ctcgcgccga acgccgtcac
      841 gaacagggac acgacgaaga ccgtcgccgc cagcctgaac ctgccgaaca ccatactttt
      901 catcatccaa ttacctctta ataactgctt ccggtttcga cgccgcaccg cacggcgcac
      961 gcgcccgtga gggccgcgct tacattaaca accgccgtgc cacgaggcgg tcgaaaataa
     1021 ggcttgtttt cggtgtttcg tgaggcgtcg ggagggcggg cacacaccaa atcgtggccc
     1081 gcgcgcgtgt atcgtgcgcg cggtcagacg atgaggttga ggtcgccgaa ctcgtgccag
     1141 aggtagccgc ggcgcaccgc gtcttcgtag gcttcgcgga tgcgctcggc gggcaggaac
     1201 gcggtcagca aatcgaggtg gctcgcctgc ggctcgtgca ggccggtgag cagaccgtca
     1261 accgccttca gctcgtgctc cgccgtcacg cgcaggcgcg tgtagccgtg gcgcgcgtgg
     1321 acgcgcccgt cgcgccccgc gactgtttcg agcgcgcgca ccacggtcgt gccgacggcg
     1381 atgatgcgac cgcccgactc gcgcgcgcgg ttgacgcgcg cggcggtctg ctcgctcacg
     1441 tagaactgct cctcggaggc cgggtgccgg gcgtcaagct cgtcgtccat gtaggacgag
     1501 aggcccgtgt gcaggacgag gtaggcggcc tcgacgccgc gccgcttgag gtcgaacagc
     1561 agccgccacg tgaaggcgcg gcccgccgag ggcatctccg ccgagccggg ttcgcgcgcg
     1621 tagacggtct ggtagtagtc caagtcccac ggcgcggaga cgtactcgta gcgcacggga
     1681 cgaccgagac gataaaggac atcgaccaac tcgtgacccg acttcgagaa gcgcaccttc
     1741 cacagtcgcg ggatatgttc gtcacgcccc tcgaccgctg ccgtcaattc ttcgccgaag
     1801 acgacgttca tccccgcgcg cagaccgcac gtgaaggggt cgcccttccg gcacaacagc
     1861 agcgcgagcc aagcgccgtc gggcaggtgc tcggccagtc tcagctcgat gcacggcccg
     1921 ccctccgcgc cgcacccttc gagcgaggcg ggcagcgtcc ggctcgtgtt gaagaccagc
     1981 aggtcgcccg cccgcaggta atcgccgagc gcgtcgaacc gcgcgtgctc gacgcgctcc
     2041 gacccgcgct cgatcaccat cagccgcacg cggtcgcgcg ccagcccccg ccgctccggc
     2101 ggctccttcg ccgacaactc cggcggcagc gtgaacgtga aaggggaaga catacgcaga
     2161 agtcggaagt cggaagtcgg aagtcggaag agaagcagaa gtgttgagcg agcggggcag
     2221 ccgttatgtt tttattccga cttccgactt ccgacttctc tttcattcca ccggcgccgt
     2281 gccggtgccg cccgtgttgt ccatctggcc gccggccccg gcgtgggtgg tggtggcgcc
     2341 ggtgaggtcg ccggtctctt cgatgtcggt gtcgtgggtc gcgggcttgt cgggttcggc
     2401 cagagcgccg cggcccgtct tgtccgtgcc gccggtgatg tccgtgccgg aggcgccgcg
     2461 gcgcgcggcg acctcgggtg ttgtatcgtt cgcgtctttc tcgtcggcca tggtgttcgt
     2521 cgccctccct cgggtgtttg agtcggactt gcttcgcccc gccggcgcgg gccacaatga
     2581 attcgtcgcc cgacaatttt ataacgcttt ccgcgtcgcg gggaaacttc atggctgcgc
     2641 ggtcggaggt cgctttcgat ggggacgttt cgcgtcgtca catacaacgt gcacaagtgt
     2701 caggggcttg accggcgggt gcggcccgcg cgcatcgcgg cggtcttgcg cgagctggac
     2761 gcggacgtga tcgccatgca ggaggtggtc agcgtcgagt cggacgcgcg gcgcgagctg
     2821 caccaggcgc gcttcatcgc cgaggagctg ggctaccact actgcgtcgg cgagaaccgg
     2881 cgccatcagg gcggcgccta cggcaacgtc atcctcacgc ggctatccat cgaggagcac
     2941 tgcaactacg acatcacgtg gcgctggcag gagccgcgcg ggtgcctgcg cgccgacgtg
     3001 cgcgtgggcg agggcaccac cgtccacgtc ttcaacgcgc acctcggcac ggccttcgtc
     3061 gagcgcagac atcaggggcg caagctcgtc tccgacgccg tcctgcgcca ctcacgcttg
     3121 cacggcccgc gcgtcctgct cggcgacttc aacgagtgga cgcgcggcct cgcctcgcgt
     3181 ctgctctcgg aagagctgtt aagccccgac ctcagccgcc acctccggcg gcg
//
LOCUS       DBNGOH010000067         1921 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2943572, whole genome shotgun sequence.
ACCESSION   DBNGOH010000067 DBNGOH010000000
VERSION     DBNGOH010000067.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1921)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1921)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1921
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2943572"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            147..1103
                     /locus_tag="AC159P_01300"
     CDS             147..1103
                     /locus_tag="AC159P_01300"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3694114.1"
                     /translation="MKIVPISVPTPFYVGPVNVYLIAEEPLTLIDTGPKTREALEALK
                     EGLRRARVRVSDIRRIVLTHAHEDHCGLAKSLRDEAKDAEVFVHGWETGHRKGRLEYA
                     EHRELLVRAGVPAGELEQMRRMYEGVRQYADALEDGEHAELVDEEEIRFEKGSLRVVH
                     TPGHTPGSCSFLREADRTLIAGDCVLKRITPNPVLSPDPVDPARRFRSLAEYLVSLAR
                     LRALSPTLVHGGHGEPVHDYEELFHRYLRAIDARQAEVVRLVPRAGATAWDISRELFP
                     GTGDVHRFLAVSEAVAHLDLAHSENKLAFELSADGREIYKKP"
     gene            complement(1219..1845)
                     /locus_tag="AC159P_01305"
     CDS             complement(1219..1845)
                     /locus_tag="AC159P_01305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694115.1"
                     /translation="MKRTLVLCLFAALMLQAPGARATRGQAQQTKPAAAAAAVPLDHG
                     GKIASEYDGFRHETVVTLKSMTVTCDKVKGAQGVIRGICVNLTASLHCPGKQLDYVRY
                     VRLQLAFEAKNWDNRHSLGERTLSAVANGETLKLGEMKLLKQEIGEGWFDERMKEVLE
                     VSVPYATFEKLALAEYVELSVGKTAFELRDKNVAALRDLNNRVRLAGR"
BASE COUNT          312 a          642 c          626 g          341 t
ORIGIN      
        1 ccgggcatcc cagcgataaa tcgccgggct attttcagac gcccctgacg gggctcacaa
       61 acgccactga cggggcggac gcgggccaga gtctctgcgg ttccctttta ccccttcccc
      121 tttccccttt tccccttttt ccccttatga aaatcgttcc catctcggtg ccaacgcctt
      181 tctacgtcgg gccggtgaac gtgtacctga tcgcggagga gccgctgacg ctgatcgaca
      241 cggggccgaa gacgagggag gcgctcgaag ctttgaagga agggttgcgg cgggcgcggg
      301 tgcgcgtctc ggacatccgg cgcatcgtgc tgacgcacgc gcacgaggat cactgcgggc
      361 tggcgaagtc tttaagagac gaggcgaagg acgccgaagt cttcgtgcac gggtgggaga
      421 cgggccaccg caaagggcgg ctcgaatacg cggagcaccg cgagctgctg gtgcgcgcgg
      481 gcgtgcccgc gggcgaactc gaacaaatgc ggaggatgta cgagggcgtg cgccagtacg
      541 ccgacgcgct cgaagacgga gagcacgcgg agctggtgga cgaggaggag atcaggttcg
      601 agaagggcag tttgcgcgtc gtccacacgc cggggcacac gcccggctcg tgctcgttcc
      661 tgcgcgaggc cgaccgcacg ctcatcgcgg gcgactgcgt cctgaagcgc atcacgccga
      721 accctgtgct ctcgcccgac cccgtagacc ccgcgcgccg cttccgctcg ctcgccgaat
      781 acctcgtcag cctcgcccgc ctgcgcgcgc tctcgccgac gctcgtccac ggcggccacg
      841 gcgagcctgt ccacgactac gaagaactct tccaccgtta cctgcgcgcc atcgacgcgc
      901 ggcaggcgga agtcgtccgc ctcgtacccc gcgcgggcgc gaccgcgtgg gacatctcgc
      961 gcgagctgtt ccccggcacc ggcgacgtgc accgcttcct cgccgtctcc gaggcggtcg
     1021 cacacctcga cctcgcacac tccgaaaaca aactcgcctt cgagctttct gccgacgggc
     1081 gcgaaatcta taaaaagccg tgaaccgtaa accgtcaacc gtgacaagtc agaagcaggt
     1141 tttcacttat cacggttcac ggctcgcggc cccgttcttt cacttatcac ggttcacggt
     1201 tgacggttca cggctttttc accttcctgc gaggcggacg cggttgttga ggtcgcggag
     1261 cgcggcgacg ttcttgtcgc gcagctcgaa ggcggtcttg ccgacggaca gctcgacgta
     1321 ttcggcgagc gccagcttct cgaaggtcgc gtaggggacg gagacttcga gcacttcctt
     1381 catccgctcg tcgaaccagc cttcgccgat ctcctgtttg agcagcttca tctcgccgag
     1441 cttgagggtc tcgccgttgg cgaccgccga cagcgtgcgc tcgccgaggg agtggcggtt
     1501 gtcccagttc ttggcctcga aggcgagctg gagacggacg tagcggacgt agtcgagctg
     1561 cttgccgggg cagtggagcg acgccgtgag gttgacgcag atgcccctga tgacgccctg
     1621 cgcacccttc accttgtcgc aggtgacggt catcgacttc agcgtgacga ccgtctcgtg
     1681 gcggaacccg tcgtactccg acgcgatctt cccgccgtgg tcgagcggca ccgccgccgc
     1741 cgccgccgcc ggtttcgtct gctgcgcctg cccccgcgtc gcgcgcgcgc ccggcgcctg
     1801 tagcatcagc gccgcgaaga gacagagaac gagtgtgcgt ttcatccgta atgaaatcct
     1861 ttcgaggtga ttgggttagg ggcgccgggg tgagatggcg actggcgcgg gcaagtgcgg
     1921 c
//
LOCUS       DBNGOH010000068         4234 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_743855, whole genome shotgun sequence.
ACCESSION   DBNGOH010000068 DBNGOH010000000
VERSION     DBNGOH010000068.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4234)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4234)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4234
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_743855"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(7..654)
                     /gene="mobA"
                     /locus_tag="AC159P_01310"
     CDS             complement(7..654)
                     /gene="mobA"
                     /locus_tag="AC159P_01310"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HIR3694116.1"
                     /translation="MIVTVSGGYILAGGASSRMGADKAALRLGGATFVERVAAALRSI
                     TSEVFVVSSKHDAARWGLPVVPDLYENSGALGGLHAALTHANTRRGAEAGGRRIAVVS
                     CDLPFVTGALMKRLASFCAPEWDAVAPVQEDGRAQPLCAVYESARCGPMADLLLDSGE
                     LRPRELLARVRTRWVAFGELAKLDGAALFFMNVNTPEEYERACEAAAHRENNPRI"
     gene            958..2841
                     /locus_tag="AC159P_01315"
     CDS             958..2841
                     /locus_tag="AC159P_01315"
                     /inference="COORDINATES: protein motif:HMM:NF017400.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIVB peptidase S55 domain-containing protein"
                     /protein_id="HIR3694117.1"
                     /translation="MKRNLVTLVALLLALHGAPASAQQKAVEAEKSAVAMMPLEEVRV
                     GQKGTGRTVFAGSEMQEFGVEVLGVMPGFPQPRMSVIIARLSGPQVERTSVFAGMSGS
                     PVYIDGKLVGAVAYAFPFSKEPIAGITPIKQMIDIFERGVRQSDGGGRDTLGFHREPR
                     AVSYNQLAGTDWKPELPRAGVAAAPFVAPVSTGSPLMPLLGQQLAPIATPVVFSGFTA
                     DALARFSPQLQSNGLLPVSGMGGASPITPMGKVTEGTLRGGTSVSVQLVRGDYSVAAS
                     GTVTLRDNERIYAFGHPFLSLGSSDMPMSESSVVTVIPNTLNSFKLTVPGQMVGSISQ
                     DRATGIYGQLRQAPKMIPVTINLHTSRERAETYKFEVASDEFLTPLLLNMTVYSTIAS
                     SERSLGDSTVSVRGKIEVKGQEPVFIERRFSMMGAGISAAGSVAAPVAALMASGFDGV
                     ELGSVTLDIAASDSRRSATLDRISLDRTEVRSGETVEVQAYVRTESGRQFVQRIPVEI
                     PADVPPGQLVLFVGDGGSLQQASNSQNFVPKDLGQLVSAINRIKKNDRLYVKLFRVTN
                     GAVIGTDEMPNLPPSFVATLNSDRAAGGYTPTVLSPVAEKELPPADYVISGQQLIGVT
                     VLR"
     gene            complement(3082..3723)
                     /locus_tag="AC159P_01320"
     CDS             complement(3082..3723)
                     /locus_tag="AC159P_01320"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF014575.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070043 - rRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HIR3694118.1"
                     /translation="MPERKRTQGEEFTDALAAGAGHYGLRLRDEELARLGDYYDTVAA
                     WNARLHLVAPCAPAEFATRHVLESLTALPLLDEGARVVDVGSGAGLPVVPCLIVRPDL
                     TATLVEASPKKAVFLREALRRVGRHTRARVLAERFERLPPPEADALTCRALERFDESL
                     PALARWAAGVRTLLLFGGPALGERIESLSLAHEALLMPDSKQRFLYVVKKDVG"
     gene            3786..>4234
                     /locus_tag="AC159P_01325"
     CDS             3786..>4234
                     /locus_tag="AC159P_01325"
                     /inference="COORDINATES: protein motif:HMM:NF012503.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HIR3694119.1"
                     /translation="MSERIAEEHERLGASVTALVAAYARTLDAARVCSTASPPELEAL
                     FDEPLPTGGAPVEEILEKFERDLLPHVMNIPSPRYYGLFNPTPLPVAVWADALASAIN
                     QNGAAWRNSPSASIVEARVLRWLCELVGYGPAAFGTLTSGGSEANLVG"
BASE COUNT          677 a         1512 c         1391 g          654 t
ORIGIN      
        1 ccggccttaa attcgcggat tattttcccg gtgggccgcg gcctcgcacg cgcgctcgta
       61 ctcttcgggc gtgttgacgt tcatgaagaa cagcgctgcg ccgtcaagtt tcgccagctc
      121 cccgaacgcg acccagcgcg tccgcacgcg cgccagcagc tcgcgcggtc gcagctcgcc
      181 ggagtcgagc agcaggtcgg ccatggggcc acacctcgcg ctctcgtaga cggcgcagag
      241 cggctgcgcg cgcccgtcct cctgaaccgg cgcgacggcg tcccactccg gcgcgcagaa
      301 agaggcgagg cgcttcatca acgcgcccgt cacgaaaggg aggtcgcagg agacgaccgc
      361 gattcgtcgc ccgcccgcct ccgcgccccg ccgcgtgttc gcgtgcgtga gcgcggcgtg
      421 aaggccgccc agcgcgccgc tgttttcgta aaggtctggg acgacgggca ggccccagcg
      481 cgccgcgtcg tgtttcgagc tgacgacgaa gacctcgctc gtgattgaac gcagcgcggc
      541 ggcgacgcgc tcgacgaaag tcgcgccgcc gagccgcagc gcggccttgt ccgcccccat
      601 ccgcgacgac gcgccgcccg cgagaatgta cccgccgctc acagtcacaa tcacaccccg
      661 acgctccacc ggcgcggacg atacgccgcc gccccggcat tagtttcgcg cgcgcccgtc
      721 aggccgggaa cagccgcttt ccgcgcgggg ctgcacggac gcgccctaac ttcaagcaac
      781 ccgtgtggtt cggcgttcat caatgagtga cgaagtgttg cttgaccgcg ttacgtgtcg
      841 aagctatagt cgaaaacggg tcgcccccgc cgctcgtttc gtgctcgtcg gaaggcttca
      901 ggcgcgtctc gactctaggt ttccagtccg tcgccgtcac cgttcagagg tcgtttgatg
      961 aaaagaaatt tagtcacgct cgtcgcactc ctcctcgcgc tccacggcgc ccccgcgtcc
     1021 gcgcagcaga aggcggtcga ggccgaaaag tccgcggtcg ccatgatgcc gctcgaagaa
     1081 gtccgtgtcg gacagaaggg cacggggcgg acggtcttcg cggggagcga gatgcaggag
     1141 ttcggcgtcg aagtgctggg cgtgatgccg ggcttcccgc agccgcgcat gtcggtcatc
     1201 atcgcgcgcc tctcggggcc gcaggtcgag cggacgagcg tcttcgcggg gatgtccggc
     1261 tcgcccgtct acatcgacgg caagctggtc ggcgccgtcg cctacgcctt cccgttctcg
     1321 aaagaaccca tcgcgggcat cacgccgatc aagcagatga tcgacatctt cgagcgcggc
     1381 gtgcgccaga gcgacggcgg cgggcgcgac acgctcggct tccaccgcga gccgcgcgcc
     1441 gtctcttaca atcaactggc gggcaccgac tggaagccgg agttgccgcg cgcgggcgtc
     1501 gccgcggcgc cgttcgtcgc gccggtctcg accggctcgc ccctcatgcc gctcctcggc
     1561 cagcagctcg cgcccatcgc gacgcccgtc gtcttcagcg gcttcacggc ggacgcgctc
     1621 gcgcgcttct cgccgcagct tcagtcgaac ggcttgctgc ccgtctccgg catgggcggc
     1681 gcatcgccca tcacgccgat gggcaaggtg acggaaggca cgctgcgggg cggcacttcc
     1741 gtgagcgtgc agctcgtgcg cggcgactac tcggtcgcgg cctcgggcac cgtgacgctg
     1801 cgcgacaacg agcgcatcta cgccttcggc caccccttcc tcagcctcgg ctcttcggac
     1861 atgccgatgt ccgagtcgtc ggtcgtcacc gtcatcccga acactttgaa ttcgttcaag
     1921 ctcacggtgc ccggtcagat ggtcggctcc atctcgcagg atcgcgcgac cggcatctac
     1981 ggccagctcc ggcaggcgcc gaagatgatc cccgtcacga tcaacctgca caccagccgc
     2041 gagcgcgccg agacctataa gttcgaagtc gccagcgacg aattcctgac gccgctgctg
     2101 ctgaacatga cggtctacag caccatcgct tcgagcgagc ggagcttggg cgattcgacc
     2161 gtcagcgtgc gcggcaagat cgaggtcaag gggcaggagc cggtcttcat cgagcgccgc
     2221 ttctcgatga tgggcgcggg catctccgcc gccgggtcgg tcgccgcgcc cgtcgccgcc
     2281 ctgatggcga gcggcttcga cggcgtcgaa ctcggctccg tcaccttaga catcgcggcg
     2341 tccgactcgc gccgctcggc cacgctcgac cgcatctcgc tcgaccgcac cgaggtgcgc
     2401 agcggcgaga ccgtcgaggt gcaggcttac gtccgcaccg agtcgggccg ccagttcgtc
     2461 cagcgcatcc cggtcgagat accggcggac gtgccgcccg gccagctcgt cctcttcgtc
     2521 ggcgacggcg gctccttgca gcaggcttcc aactcgcaga acttcgtgcc gaaggatttg
     2581 ggccagctcg tcagcgccat caaccgcatc aagaagaacg accggctcta cgtcaagctc
     2641 ttccgcgtca ccaacggcgc cgtcatcggc accgacgaga tgccgaacct gccgccctcg
     2701 ttcgtcgcca cgctgaacag cgaccgcgcc gccggcggct acacgccgac cgtgctctcg
     2761 cccgtcgccg agaaagagct gccgcccgcc gactacgtca tcagcggcca gcaactcatc
     2821 ggcgtcacgg tcttacgctg aagcagtttt aaattcatcg acggagaggt gagcctggcg
     2881 ccacgcggct cacctcttct gtttttgtca gagccgcgta gcggcgcagt gaatgtaacc
     2941 cggccttgaa aggccgggtt agtcgcaacg acacggcccg cgtcgcgaca gcgacgccat
     3001 gaccatgtga cgcgcatccc cggccttgaa aggccgggct acattcaacc gccgctacgc
     3061 ggcgattcga acggtcggca cctatccaac atctttcttg acgacgtaga gaaaacgctg
     3121 tttcgagtcc ggcatgagca aggcttcgtg ggcgagcgac agcgactcga tcctttcgcc
     3181 gagcgcgggg ccgccgaaga ggagcagcgt ccgcacgccc gcggcccaac gggcgagcgc
     3241 gggcaggctt tcgtcgaagc gttcgagggc gcggcaggtg agggcgtccg cctcgggcgg
     3301 cggcaggcgc tcgaaccgtt cggcgaggac gcgcgcccgc gtgtgacgcc cgacgcgccg
     3361 cagcgcttcg cgcaggaaga cggccttctt cggcgaggct tcgacgagcg tcgccgtgag
     3421 gtcggggcgc acgatcagac aagggacgac gggcaatccc gcgccggagc cgacatcgac
     3481 gacgcgcgcg ccttcgtcga gcagcggcag cgcggtgagc gattcgagca cgtgccgcgt
     3541 ggcgaactcc gcgggcgcgc agggggccac cagatggagc cgcgcgttcc acgccgcgac
     3601 cgtgtcgtag tagtcgccga gccgcgccag ctcttcgtcg cgcaaacgca ggccgtagtg
     3661 cccggcgccg gccgcgagcg cgtcggtgaa ctcctcgcct tgtgtccgct tccgttcggg
     3721 catcgctatc gaataggatg agaacagctt aacgaaagtg ccgccggatg ggaaacagcc
     3781 gaccgatgag tgaacgaatc gcggaagaac acgagcggct gggggcgagc gtgaccgcgc
     3841 tcgtcgcagc ctacgcgcgc acgctcgacg cggcgcgcgt ctgctcgacg gcctcgccgc
     3901 ccgaactcga agcgctcttc gacgagccgc tgcccacggg cggcgccccg gtcgaagaga
     3961 ttctggaaaa gttcgagcgc gacctgctgc cgcacgtgat gaacatcccc agcccgcgct
     4021 actacgggct gttcaacccg acgcctttgc ccgtcgcggt gtgggccgac gcgctcgcct
     4081 ccgccatcaa ccagaacggc gcggcgtggc gcaactcgcc atcggcctca atcgtcgagg
     4141 cgcgcgtcct gcgctggctc tgcgagctgg tcggctacgg ccccgcggcc ttcgggacgc
     4201 tgacgagcgg cggctccgag gcgaacctcg tcgg
//
LOCUS       DBNGOH010000069         3004 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2027150, whole genome shotgun sequence.
ACCESSION   DBNGOH010000069 DBNGOH010000000
VERSION     DBNGOH010000069.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3004)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3004)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3004
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2027150"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            488..1750
                     /locus_tag="AC159P_01330"
     CDS             488..1750
                     /locus_tag="AC159P_01330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694120.1"
                     /translation="MSKLCITLTLAALACFVSPVSRGQTAPPQPAAAPRATQQHLIGE
                     VTAIDQAAGLITVRTDAGATVNVKTDERTLYRRLPPGETSLQKAEQITRADLRAGDRV
                     LVPGALAAGEGATVQQVIVTAREAASERREREDWRRRGVAGRVVALDEGRKTIVLETR
                     GGRDAARAVTVDAADAGVRFLRYAPDSFRRADARPGAFPDIAVGDQLRALGERSADGS
                     RVKAEEVVSGTVERIVGAVTSVDLARGEVSVKNEQTGQTVVITVGRNTTLRRVPREVA
                     EELAARSEQREARRGERDDDAQRREERRNQRREERRRENAADDSTAPQSGGGQRARPA
                     GRSVQQIFASLPVIKVEDLKKGDTAVVTATVGEDAARATAVSLIAGEASMLRWLQRFQ
                     RVADDLRDMTTGLPGTVMGGNTGSNDEP"
     gene            1847..>3004
                     /locus_tag="AC159P_01335"
     CDS             1847..>3004
                     /locus_tag="AC159P_01335"
                     /inference="COORDINATES: protein motif:HMM:NF051698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR3694121.1"
                     /translation="MRTFRMTGRRVLAQSLLLLLFAAAAAFAQQSQATLRGQVADALG
                     GVVVGATVTAADASGVERTATTDEEGRYVFPSLAPGRYAVRVAAPGFTLYENLEVEVT
                     AGRTEPLDVVLTVAIEEEVVTVTAEADVGTDPEASAGAVVLRGTDLDSLPDDPEGLAE
                     ALQALAGPAAGVDGEAQIYLDGFTGGRLPPKESIREIRINRNPFSAEFDRLGYGRVEI
                     FTKPGTDKYRGQTFFNFNDEALNSRSPFAAVRAPFQSRRYGGNLSGPISARKASFFFD
                     FQRSETDDNQDINAVVLDPSLAPVPFITTVLTPQRRTTFSPRFDYQLNQTNTLVARYS
                     YERTRRENGGVGGFNLPERAFDTGVTQQTVQLTETAVINQKVVNETRFQYER"
BASE COUNT          517 a         1092 c          975 g          420 t
ORIGIN      
        1 accgccgcac gaacgcgacc cgcctgcacg tgcccccgtt agtgacggtt cgcggttgag
       61 tctaccgtgc gttggttttc aacacagagg gcacggagga gacacagagg acacagagaa
      121 agacctcttc ttctctgtgc tctccgtgtg acctctgtgc cctctgtgtt gaaacgaacc
      181 cgcgctatgc tcaaccacaa tttgttcatc gcttatcggt ctataaaaat tattcgccgc
      241 acgcggccca cgatttccgt aaccgtcgcg cggcccgcgg cgtccctctc tctgtaagac
      301 ctgcgttcga gaggggccgt gcttcgccgc cgatggtgac aaacgacgcg cttcgccata
      361 gaatgcgcga aggcattccc cctgtacccc gagccaaacg aaagccgacc gaagccgcaa
      421 cggcgacttc tcgtcaacct ccgcgcgcga gggcgtgcgg ctctccaaca agcaaccggt
      481 gaagacgatg tccaagctct gtatcactct cacgctcgcc gcgctggcgt gcttcgtgtc
      541 gccggtctcg cgcgggcaga ccgcgccgcc gcaaccggcc gccgcgccgc gcgccacgca
      601 gcaacacctg atcggcgagg tcacggcgat agatcaggcc gccggtctca tcactgtgag
      661 gacggacgcg ggcgcgaccg tcaacgtcaa gaccgacgag cggactctgt atcgtcgcct
      721 gccgccgggc gagacctctt tgcagaaggc cgagcagatc acgcgcgccg acctgcgcgc
      781 gggcgaccgc gtgctcgtgc cgggcgcgct cgccgcgggc gaaggggcga ccgtccagca
      841 ggtcatcgtc acggcgcgcg aggccgcctc ggagcgacgc gagcgcgaag actggcggcg
      901 gcgcggcgtc gcgggccgcg tcgtcgcgct cgacgaaggc cgcaagacaa tcgtccttga
      961 gacgcgcggc gggcgcgacg cggcgcgcgc cgtcacggtg gacgccgcgg acgcgggcgt
     1021 gcgcttcctg cgttacgcgc ccgactcttt ccgccgcgcc gacgcgcgcc ccggcgcctt
     1081 ccccgacatc gcggtcggcg atcagctccg cgccctcggc gagcgcagcg ccgacggctc
     1141 gcgcgtcaag gccgaagagg tcgtctccgg caccgtcgaa aggatcgtcg gcgccgtcac
     1201 ctcggtcgat ctcgcgcgcg gcgaggtctc cgtgaaaaac gaacagacgg gacagacggt
     1261 cgtgattacc gtcggcagga acaccacgct gcgccgcgtc ccgcgcgagg tcgcggagga
     1321 actcgccgcg cgcagcgagc agcgcgaagc gagacgcggc gagcgcgatg acgacgcgca
     1381 gcggcgcgag gagcgacgca accagcggcg tgaagagcga cgcagagaga acgccgccga
     1441 cgattcgacc gcaccacaat caggcggcgg gcaacgtgcg cgccccgcgg gccgcagcgt
     1501 ccagcagatt ttcgcgagcc tgcccgtcat caaggtcgaa gacctgaaga agggcgacac
     1561 cgccgtcgtc acggccacgg tcggcgaaga cgcggcgcgc gccaccgccg tctcgctcat
     1621 cgcgggcgag gcgagcatgc tccgctggct gcaacgcttc cagcgcgtgg cggacgatct
     1681 gcgcgacatg actacgggcc tgcccggcac cgtcatgggc ggcaacacgg gcagcaacga
     1741 cgagccgtaa gaaccggacg gcatttcaca ccttgtagac gaacgacgag ccgcccgccg
     1801 acggaagaga tgacgaacga tgggccgtgc tgcaactgaa aagatgatgc gtacatttcg
     1861 tatgacgggt cggcgcgttc tcgcgcagtc cctcttgctc ttgctcttcg cggcggcggc
     1921 ggccttcgcg cagcagtcgc aggcgacctt gcgcgggcag gtggccgacg cgctcggcgg
     1981 cgtggtggtc ggcgcgaccg tcaccgcggc ggacgcttcg ggcgtcgagc gcacggcgac
     2041 gaccgacgaa gagggccgct acgtcttccc ctcgctcgcg cccggtcgct acgcggtgcg
     2101 cgtcgcggcg ccgggcttca cgctctacga gaaccttgaa gtcgaagtca ccgccggtcg
     2161 caccgagccg ctcgacgtgg tgctcaccgt cgccatcgag gaggaggtcg tcaccgtcac
     2221 cgccgaggcg gacgtgggca cagaccccga ggcgagcgcg ggcgccgttg tcctgcgcgg
     2281 cacagacctc gactctctgc cggacgaccc cgaaggtctc gccgaagcgt tgcaggctct
     2341 ggccggcccc gccgcgggcg tggacggcga ggcgcaaatc tacctcgacg gcttcacggg
     2401 cgggcggctg ccgccgaagg aatccatccg cgagatacgc atcaaccgca accccttctc
     2461 cgccgagttc gaccgcctcg gctacggtcg cgtcgagatt ttcaccaagc ccggcacgga
     2521 caagtatcgc ggccagacct tcttcaactt caacgacgag gcgctgaact cgcgctcgcc
     2581 cttcgccgcc gtccgcgcgc ccttccagtc gcgccgctac ggcggcaacc tctccggccc
     2641 catcagcgcg cgcaaggcct ccttcttctt cgacttccag cgcagcgaga cggacgacaa
     2701 tcaggacatc aacgccgtcg tcctcgaccc ctcgctcgcc cccgtgccct tcatcaccac
     2761 ggtgctcacg ccgcagcggc gcacgacctt cagcccgcgc ttcgactacc aactgaacca
     2821 gacgaacacg ctcgtcgcgc gctactctta cgagcgcacg cgccgcgaga acggcggcgt
     2881 cggcggcttc aatctgcccg agcgcgcctt cgacacgggc gtcacgcagc agaccgtcca
     2941 gctcacggag accgccgtca tcaaccagaa ggtcgtcaac gagacgcgct tccagtacga
     3001 gcgc
//
LOCUS       DBNGOH010000070         3500 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3127103, whole genome shotgun sequence.
ACCESSION   DBNGOH010000070 DBNGOH010000000
VERSION     DBNGOH010000070.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3500)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3500)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3500
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3127103"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1231
                     /locus_tag="AC159P_01340"
     CDS             <1..1231
                     /locus_tag="AC159P_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HIR3694122.1"
                     /translation="DLPTQSWGAGPLVQYMLGWLHERCGETGAARAAYRRGAAHPPDY
                     CFPSRLEEIAVLESAARAEPRDARAPYYLGNLLYDRRRHAEAVRLWERSAELDPAFSV
                     VWRNLGLGYFNVGKKPGKARTAYDRAFAANPSDARLLYERDQLWKRTGRSPARRLAEL
                     EKHRELVRQRDDLAVELCALYNQTGRHDEALALLEARQFQPWEGGEGQALGQHVRTHL
                     ALGRRALKAFDAAAARVHFEAALTSPRNLGEAKHLLANQSDIHYWLGAALDALGDRAA
                     AERHWTAAATFRGDFQEMSVREFSEMTYYSALSLRRLGREAEANELLRGLEGYADGML
                     RSEAKIDYFATSLPTMLIFEDDIQERQRVGATLLKALAKLGRRRKKEGLRLLDEVLRS
                     DPNHAFAKDVRDEVGGG"
     gene            1249..2226
                     /locus_tag="AC159P_01345"
     CDS             1249..2226
                     /locus_tag="AC159P_01345"
                     /inference="COORDINATES: protein motif:HMM:NF024799.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HIR3694123.1"
                     /translation="MRIVLNPYCLLAALVAALVFNGCGRSGGPGEQSRPLKLAVVTNN
                     SSDWWTIARKGGEKAAGELPGVSLDFRMLSEGTAAAQKALVDDLLARGVDGIAVSPLD
                     VENQRQMLDEAASKTLLFTLGADAPASRRVCYAGTDDVASGRMAGALVRQTLPEGGKI
                     MVFVGRRDAQNAIDRFNGLKQGLEGSGVEIIDVRTDDADPTKAKTNVADTLVRYPDID
                     GLVGLWSYNGPAILSAVADAGKLGKFRIVTFDEADETLQGVKDGHIYGTVVQQPYGFT
                     YEAVKRMALYLRGDKSQIPADGKLVYPTEVIRQENVDEFRAKLKELRGR"
     gene            2275..>3500
                     /locus_tag="AC159P_01350"
     CDS             2275..>3500
                     /locus_tag="AC159P_01350"
                     /inference="COORDINATES: protein motif:HMM:NF014851.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding domain-containing protein"
                     /protein_id="HIR3694124.1"
                     /translation="MNRDTRNAAQLCLLAAVLCLLTPAGLRASEPIQLEGTWRFALDR
                     ADAGVKEAWFGRSLPDRISLPGILQSQGYGDEISTQTPWVLSLYDRHWFLREEYEAYT
                     RAGNVKVAFLPQPPRHYVGVAWYQRDVDIPDEWRGRRVVLFMERPRWESTMWVDDRRV
                     GSNNSLVAPHEFDLGRLEPGRHRLTVRVDSSMLLPYRPDAHSVSDSLGSSWNGIVGKI
                     ELTATSPVWMEDAQVYPEVSKRSARVVVRVGNVTGRAGEGTLSAAGVSAPVAWDAGGG
                     RAELEVPLGPNAKTWDEFSPFVHRLTLQLKGVQADDSREVVFGLREFRAEGGEFLVNG
                     HKTNLRGTHMGGDFPLTGYPATDVESWRRILRTCKQWGLNHVRFHSWAPPEAAYVAAD
                     ELGVYLQPEPGMWNEI"
BASE COUNT          613 a         1148 c         1213 g          526 t
ORIGIN      
        1 cgaccttccc acgcagagct ggggggccgg gccgctcgtc caatacatgc tcggttggct
       61 gcacgagagg tgtggcgaga cgggcgcggc gcgggccgcg tatcgtcgcg gggcggcgca
      121 cccgccggac tactgcttcc cgtcccgcct cgaagagatc gccgtcctcg aatcggccgc
      181 gcgcgctgaa ccgcgcgacg cccgtgcccc ttactacctc ggcaacctcc tctacgaccg
      241 gcgccgccac gccgaggccg tccgactttg ggagcgcagc gccgaactcg acccggcctt
      301 ctccgtcgtc tggcgcaacc tggggctggg ctacttcaat gtcggcaaaa agcccggcaa
      361 ggcgcgcacg gcctacgacc gcgcgttcgc ggcgaatccg tcggacgcgc gcctccttta
      421 cgagcgtgac cagctctgga agcgcacggg caggtcgccc gcacgtcgcc tcgcggagct
      481 tgagaagcac agggaactcg tccgccagcg cgacgacctc gccgtcgagc tgtgcgcgct
      541 ctacaaccag acggggcggc acgacgaggc gctggctctt ctcgaagcgc gccagttcca
      601 gccgtgggag gggggcgaag gccaggcgct cggccagcac gtgcgcacgc atctggcgct
      661 gggccgccgc gcactcaaag cgttcgacgc cgccgcggcg cgcgttcatt tcgaggcggc
      721 gctcacgtcg ccgcgcaatc tcggcgaagc caaacacctc ctcgccaacc agagcgacat
      781 acactactgg ctgggcgcgg ccctcgacgc gctcggcgac agggcggcgg cggagcgaca
      841 ctggacggca gccgccacct tcagaggcga cttccaggag atgagcgtgc gcgagttctc
      901 cgagatgacc tactactcgg cgctctcgct cagacggctc ggccgcgagg cggaggccaa
      961 cgaactgctt cgcggcctcg aaggttacgc cgacgggatg ctccgttcgg aggcgaaaat
     1021 agactacttc gcaacgtctc tgcccacgat gctgatcttt gaggacgaca tacaggagcg
     1081 tcagcgggtg ggcgcgacgc tgctcaaggc cctggcgaag cttggcagga ggaggaagaa
     1141 ggaggggttg aggctgctcg acgaggtcct gcgcagtgac cccaaccacg cgttcgcgaa
     1201 agacgtcagg gatgaagtgg ggggcggtta aagatgaagg agacgttgat gagaatcgtg
     1261 ctgaatccgt attgcttgct ggccgcgctc gtcgccgcgc tcgtcttcaa cgggtgcggc
     1321 aggtcgggcg ggccgggcga gcagtcccgt ccgctcaagc tggccgtcgt caccaacaac
     1381 agctccgact ggtggaccat cgcgcgcaag ggcggcgaga aggccgcggg cgagctgccc
     1441 ggcgtgagtc tcgacttccg catgctctcc gaaggcactg ccgccgcgca gaaggcactc
     1501 gtcgatgacc ttctcgcacg gggtgtggac ggtatcgccg tcagccctct ggacgtggag
     1561 aaccagcggc agatgctgga cgaggcggcc tcgaaaaccc tgctgttcac actcggcgcg
     1621 gacgcccccg caagccgccg ggtctgttac gcggggacgg acgacgtggc ctccggtcgc
     1681 atggcgggcg cgctggtcag gcagacactg ccagagggag ggaagatcat ggtcttcgtg
     1741 ggccgccgcg acgcgcagaa tgcaatcgac cgcttcaacg gcctgaagca gggcctggag
     1801 ggcagcggcg tagagattat cgacgttcgc acggacgacg ccgacccgac gaaggcgaag
     1861 acgaacgtgg ccgacacgct cgtcaggtac ccggacatcg atggcctggt cgggctgtgg
     1921 agctacaacg ggccggcgat cctgagcgcg gtcgcggacg ccggcaagct cggcaaattc
     1981 cgcatcgtca ccttcgacga agcggacgag acgctccaag gggttaagga cggacacatt
     2041 tacggcactg tcgttcagca gccctacggg ttcacctacg aggccgtcaa gcggatggcc
     2101 ctctacctgc gcggcgacaa gagccagatt ccggcggacg gcaaactcgt ctacccgacc
     2161 gaggtcatcc gtcaggagaa cgtggacgag tttcgggcga agctgaaaga gctgcgcggg
     2221 cgctgacgtg atagttccgc cacgtggcgg aactgaaggc cggatgaatt gacgatgaac
     2281 cgagataccc gtaacgccgc ccaactctgt ctgctggccg ccgtgctctg tctgctgact
     2341 cccgcggggc tgcgggcgtc agagcccatc cagctcgaag gcacctggcg gttcgcgctg
     2401 gatcgggcgg acgcgggcgt gaaagaagcg tggttcggcc gcagcctgcc cgaccggatt
     2461 agtctccccg gcatcttgca gtcgcaaggc tacggcgacg agatcagcac gcaaacgccc
     2521 tgggtgctct cgctctacga ccgtcactgg ttcctgcgcg aggagtacga ggcttacacc
     2581 agggccggca acgtcaaggt ggcgttcctg ccacagcctc cacgccacta cgtcggcgtg
     2641 gcctggtatc agcgcgacgt ggacatcccc gacgagtggc gcgggcgccg cgtcgtactc
     2701 ttcatggagc ggccccgctg ggagtcaacc atgtgggtgg acgaccgccg cgtcggctcg
     2761 aacaacagcc tcgtcgcgcc gcacgagttc gacctcggcc gtctcgagcc gggccgccac
     2821 cgcctcacgg tgcgtgtcga ttcctcgatg ctgctgccgt acaggccgga cgcgcacagc
     2881 gtctcagact ccctgggcag cagttggaac ggcatcgtcg gtaagatcga gctgacggcg
     2941 accagtcccg tctggatgga ggatgcgcag gtctatcccg aggtgtccaa aaggtcggcg
     3001 agggttgtgg tgcgggtcgg caacgtgacg ggccgggcgg gcgaagggac tctgtcggcg
     3061 gccggcgtca gtgcgcccgt cgcttgggac gccggaggcg ggagggccga gttggaagtg
     3121 ccgctcgggc cgaacgcgaa gacttgggac gagttcagcc ccttcgtgca ccgcctcacg
     3181 ttacagctca agggcgtgca ggcggacgac agccgcgagg tcgtcttcgg gctgcgcgag
     3241 tttcgggccg aaggcggcga gttcctcgtc aacgggcaca agacgaacct gcgcggcaca
     3301 cacatgggcg gcgatttccc gctgacgggc tacccggcga cagacgtcga atcgtggcgc
     3361 cgcatcctgc gcacatgcaa gcagtggggt ctgaaccacg tccgcttcca ctcttgggcc
     3421 ccgcccgaag ctgcctacgt cgcggcggac gaactcggcg tctacctcca gcccgagccc
     3481 ggcatgtgga acgagatcga
//
LOCUS       DBNGOH010000071         2317 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1660786, whole genome shotgun sequence.
ACCESSION   DBNGOH010000071 DBNGOH010000000
VERSION     DBNGOH010000071.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2317)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2317)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2317
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1660786"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..325)
                     /locus_tag="AC159P_01355"
     CDS             complement(<1..325)
                     /locus_tag="AC159P_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018137176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenosugar biosynthesis radical SAM
                     (seleno)protein ArsS"
                     /protein_id="HIR3694125.1"
                     /translation="MPQELPVIQTPRAKAPPPHYDFDAVLAARGVELLRADAIEIFQV
                     NVGKLCNQACKHCHVDASPARTEVMTREVAAEVVGAVQRFRFPVVDITGGAPELNPSF
                     RYLVAE"
     gene            complement(325..654)
                     /locus_tag="AC159P_01360"
     CDS             complement(325..654)
                     /locus_tag="AC159P_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014810615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenosugar biosynthesis-associated
                     peroxidase-like protein"
                     /protein_id="HIR3694126.1"
                     /translation="MSYYEEDDLQRFSEVGRHRPDLFEKFMAWYEACQQDGALSRREK
                     ALIGLAVAHALQCPYCIDAYAQSCLEAGSNMEQMTEAVHMASALRGGASLIHGLQAHN
                     SVNKVSM"
     gene            763..1437
                     /locus_tag="AC159P_01365"
     CDS             763..1437
                     /locus_tag="AC159P_01365"
                     /inference="COORDINATES: protein motif:HMM:TIGR04282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04282 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HIR3694127.1"
                     /translation="MVAAVVVMVKAPRAGFVKTRLVPPLSAEEAAALAACFVKDAVRG
                     AGLSACEVVIAYTPADGRATLEALLPGGLRWSRQRGQDLGERLEHAAAEGFARGPGPL
                     VIIGADSPTLPPSFITRAAGALAADEADIALGPTADGGYYLVGLRHPAPGLFRDIAWS
                     TPHAYAHTARNAARLNLRLLELPPWYDVDTPADLSRLRAELAADEAARRRAPATSRWL
                     TKSREP"
     gene            complement(1553..>2317)
                     /locus_tag="AC159P_01370"
     CDS             complement(1553..>2317)
                     /locus_tag="AC159P_01370"
                     /inference="COORDINATES: protein motif:HMM:TIGR04283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04283 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HIR3694128.1"
                     /translation="PSAARPAAAVMNMKREEPGGGEASLSIIIPTLDEARSIGATLDA
                     VAAVRGVAEVIVADGGSRDGTLEIVRARGVRVVECGRGRGLQMHAGAAAARGRALWFL
                     HADTLAPPDAAERIAAALADARVAGGNFHIRFDGERRAARFLTWLYPRLRRLGLCYGD
                     SGIFVRRDAYERVGGFNHFPIFEDLDLVRRLRRAGRVVHLPATVVTSSRRFEGRSFAL
                     TFARWSALQTLYWLGVHPRRLARLYAPVRKAVNHEP"
BASE COUNT          360 a          827 c          784 g          346 t
ORIGIN      
        1 actccgcgac gagatagcgg aacgaagggt tgagttcggg cgcgccgccc gtgatgtcga
       61 cgacggggaa gcggaacctc tgcacggccc cgacgacctc cgcggccacc tcgcgcgtca
      121 tcacctcggt gcgcgcggga ctcgcgtcca cgtggcagtg cttgcacgcc tggttgcaga
      181 gcttgccgac gttgacctga aaaatctcta tcgcgtcggc gcgcaggagt tcgacgccgc
      241 gcgccgcgag cacggcgtcg aaatcgtagt gcggcggcgg cgccttcgcg cgcggcgtct
      301 ggatgacggg cagctcctgc ggcatcacat cgacactttg ttgacggagt tgtgcgcctg
      361 tagcccgtgg atgagcgacg cgccgccgcg cagcgccgag gccatgtgga cggcttcggt
      421 catctgctcc atgttcgagc cggcttcgag gcacgactgc gcgtacgcgt cgatgcagta
      481 agggcactgc aacgcgtgcg ccaccgccag cccgatcaga gccttctccc gccgcgacag
      541 cgccccgtcc tgctggcacg cctcgtacca cgccatgaac ttctcgaaca ggtcgggccg
      601 gtgcctgccg acctccgaga agcgttgcag atcgtcttcc tcgtaatagg acatgtgcga
      661 tactccaaaa cagaagtcag aggtcagaag tcagaagtca gaagagaagc gctcggccac
      721 tcattctgtc ttctgtctcc tgacttctga ctcctcattc ttatggttgc cgccgtcgtc
      781 gtcatggtca aggccccgcg cgcggggttc gtgaagactc gcctcgtgcc gccgctgtcg
      841 gcggaagagg cggcggcgct cgccgcgtgc ttcgtaaagg acgcggtgcg gggcgccggg
      901 ctgtctgcgt gtgaagtggt catagcttac acccccgcgg acgggcgggc cacgctcgaa
      961 gcgctgttgc ccggcggcct gcgctggtcg cggcagcggg gccaagacct cggcgagcgg
     1021 ctcgaacacg ccgccgccga aggcttcgcg cgaggccccg gcccgctcgt catcatcggc
     1081 gccgacagcc cgacgctccc gccgtccttc atcacgcggg ccgcgggcgc gctcgcggcg
     1141 gacgaggcgg acatcgcgct cgggccgacc gctgacggcg gctactacct cgtcggttta
     1201 cgccaccccg cgcccggcct cttccgcgac atcgcgtgga gcacgcccca cgcctacgca
     1261 cacaccgcgc gcaacgccgc gcgcctcaac ctgcgcctgc tcgaactccc cccctggtac
     1321 gacgtggaca cgcccgccga cctctcacgc ctccgcgccg aactcgccgc cgacgaagcc
     1381 gcacgccgac gcgcgcccgc cacctcccgc tggctgacta aaagccgtga accgtgaacc
     1441 gtgaaccttg aaccgtgatt aagtgaaaag cagtaaggag tgatgagggg gatgctgtac
     1501 ggactcctca cggctcatgg ctgacggcta acgattaacg gctttcctct tgtcacggtt
     1561 catggttcac ggcttttctc accggcgcgt atagtcgggc gaggcggcgg gggtggacgc
     1621 cgagccagta gagcgtttgc agggccgacc agcgcgcgaa ggtgagggcg aagctgcggc
     1681 cctcgaagcg gcgcgaggag gtgacgacgg tcgcgggcag gtggacgacg cggcccgcgc
     1741 ggcgcaggcg tcgcaccaag tccaagtcct cgaagatggg gaagtggttg aagccgccga
     1801 cccgttcgta ggcgtcgcgc cgcacgaaga tgccggagtc gccgtagcag agtccgagcc
     1861 ggcgcagacg cgggtagagc cacgtcagga agcgcgcggc cctgcgctcg ccgtcgaaac
     1921 ggatgtggaa gttgccgccc gccacccgcg cgtcggccag cgccgcggcg atgcgttcgg
     1981 cggcgtccgg cggcgcgagc gtgtccgcgt gcaggaacca gagcgcacgc ccgcgcgcgg
     2041 cggcggcccc cgcgtgcatc tgtaggccgc gcccgcgccc gcactcgacg acgcgcacgc
     2101 cgcgcgcacg cacgatctcc aacgtcccgt cgcggctgcc gccgtccgcg acgatcacct
     2161 cggcgacgcc gcgcaccgcc gccacggcgt cgagcgtcgc cccgatggag cgcgcctcgt
     2221 cgagcgtcgg gatgatgatc gagagcgacg cctccccgcc tcccggctct tcgcgcttca
     2281 tgttcatcac ggcagcagcc ggtcgcgcgg cgcttgg
//
LOCUS       DBNGOH010000072         3870 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1294234, whole genome shotgun sequence.
ACCESSION   DBNGOH010000072 DBNGOH010000000
VERSION     DBNGOH010000072.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3870)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3870)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3870
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1294234"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1301)
                     /locus_tag="AC159P_01375"
     CDS             complement(<1..1301)
                     /locus_tag="AC159P_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR3694129.1"
                     /translation="MKNQGGLEMIEQGGEKFAAFERYNENLSDEAISTHDIDDVLHKL
                     EASSISGTESDEELISEVASLSTPEDEEVALDEVDELDLSAGEDEKSADPVRVYLRQM
                     GTVPLLTREQEVSIAQRIERGQIKTHKAISRSPIAVEELLRIGEGLQTGRLNIRDVVN
                     FADQAEVAEAEDKADEYLQWTLEGIENINKHFREGLKEWAKLAAEQKLHRGKKTKKLL
                     RLTRKLALTRLDISREIKNLNLKESARQRLIDAIAAAFKEIRSAEREIAKLTDKLEAK
                     RIKPEVQKELKRQLLAAKRRYKKVEEDHNVSPVEIKRTHQSIIIGELETHNAKRELTE
                     ANLRLVVSIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEWRRGYKFSTYATWWIRQAV
                     TRAIADQARTIRIPVHMIETINKLIRTSRALVQEFGREPTT"
     gene            1737..3800
                     /locus_tag="AC159P_01380"
     CDS             1737..3800
                     /locus_tag="AC159P_01380"
                     /inference="COORDINATES: protein motif:HMM:NF016289.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HIR3694130.1"
                     /translation="MRKITALLVCLGFFTIPASANDSTLLPEQVVAALAQELSGETAK
                     RNLEFLARHHRMRGSRGFRAAAEHLAEQLRAYGLADARIEQFPADGKIFYGTQRSRPP
                     WDADFAELWEVRGDGLVSTPTRWLPVARLASWEAMPVTLAQDSESADVTVELVDVGGG
                     VTERDYASRDVRGKIVLAASQPGPVARLAVEKYGALGIVSYAQNQRTAWWGENDRLVR
                     WGHLDTFAAKPTFAFMVSLKQARELQGRMARAERVWLRAVVRAGKHAGAYDVLTATIP
                     GADPRLKDEEIAFSCHLDHQRPGANDNASGSVAILEVARTLSKLVREGRIPPPARTIR
                     FIWPPEIEGTLALLNARPDIAARTKAVIHMDMVGGGPETKSVFHVTRSPASLPSFVND
                     VAEHFGEFVNEQSARFAGGESVPYSLHSPEGGKEALLAEMAEFSSGSDHQIYTDSSWG
                     IPAIYLNDWPDRYIHTNFDTPANVDPTKLKRAAFIGAASGYFLADTKADDAPALLRLF
                     RSQALRRTARMLTRRDSLPPAEAANLTRFHRWYERAVLASMSRFFKVPESVGATGAYF
                     LDTLQTITGPPSPLPPTPAGDGRVVFRRNAKLKGPMSAFGYDYFTDKLGEERVRQVRL
                     LRFEGARGSGGDYAYEVLNFVDGRRTAQEIRDAVSAVYGPVPLETVVEYLRALETIGA
                     VESNR"
BASE COUNT          613 a         1312 c         1229 g          716 t
ORIGIN      
        1 tcggtcgtcg gctcgcgccc gaactcctgc acgagggcgc gcgaggtgcg gatgagcttg
       61 ttgatcgtct cgatcatgtg gacggggatg cggatggtgc gcgcctgatc cgcaatcgcg
      121 cgcgtgacgg cctgccgaat ccaccacgtg gcgtaggtcg agaacttgta gccgcggcgc
      181 cactcgaact tatcgaccgc cttcatgagg ccgatgttgc cctcctgaat caggtcgagg
      241 aactggaggc cgcggttcgt gtacttcttg gcgatggaga cgacgaggcg caggttggct
      301 tcggtcagct cgcgcttggc gttgtgcgtc tcaagctcgc cgatgatgat cgactggtgc
      361 gtgcgcttga tctcgacggg cgagacgttg tggtcttctt cgaccttctt gtagcgacgc
      421 ttggcggcga ggagctgccg cttcagctcc ttctgcactt cgggcttgat gcgcttggct
      481 tcgagcttgt ccgtgagctt ggcgatctcg cgctcggcgg agcgaatctc cttgaaggcg
      541 gcggcgatgg cgtcgatcag acgctggcgc gcgctctcct tcaggttgag attcttgatc
      601 tcgcgcgaga tgtcgaggcg cgtcagggcg agcttgcgcg tcagccgcag cagcttcttc
      661 gtcttcttgc cgcggtggag cttctgctcg gcggcgagct tcgcccactc cttcagccct
      721 tcgcggaagt gcttgttgat gttctcgatg ccttcgagcg tccactgcaa atactcgtcg
      781 gccttgtcct cggcctcggc gacctccgcc tggtcggcga agttcaccac gtcgcggatg
      841 ttgagccgcc cggtctggag accctcgccg atgcggagca gttcctcgac cgcgatgggc
      901 gagcgcgaga tggccttgtg cgtcttgatc tggccacgct cgatgcgctg cgcaatggag
      961 acctcctgct cgcgcgtcag gagaggcacc gtgcccatct ggcgcaggta gacgcggacg
     1021 gggtcggcgc tcttttcgtc ctcgcccgcc gacaggtcga gttcgtcaac ctcgtcgagc
     1081 gcgacctcct cgtcctccgg cgtggagagc gaggcgacct cggagatcag ctcctcgtcg
     1141 gactcggtcc ccgaaatgct cgacgcttcg agtttgtgca gcacgtcgtc gatgtcgtgc
     1201 gtcgagatgg cctcgtccga gaggttctcg ttatagcgtt cgaacgcggc gaacttctcg
     1261 cccccctgct cgatcatttc cagtccgccc tgattcttca tctttgtgtc caatttaacc
     1321 tcacatggaa ggttcaagtt atgaatgcaa ctgatacgcg gcagccgact tttttgtttt
     1381 ctacagcgac tggccgccgc cgtcgctccc cgcccctcgc ccgcctacaa ttccgtccgt
     1441 aactaaggtc ttggcacggc tgaatttgga tgccgacgac ggatttcccg ttgtagcacc
     1501 gaaaagggtc ttatacatga ataccgccaa agctccaagc cctaaaacga aatgctacaa
     1561 tcaggcgccc cgcacgcaag cgccgatggc cgccggagcc gcccaaaggc tggcatcgtt
     1621 cgggttaacc ggcttttccg ggagaagacg gcgcgaggcc ggttttcgac ggtcgcgccg
     1681 cccgtcgcat tatgaccgga tcacagtctc gataaagtct cgatcaggag aaacctgtga
     1741 gaaagattac cgcgctgctc gtctgcctcg gtttttttac aattcccgcg agcgcgaacg
     1801 actccacgct gctgccggag caggtcgtcg ccgcgctcgc gcaggagctt tcgggcgaga
     1861 cggccaagcg caatctggaa ttcctcgccc gccaccaccg tatgcgcggc tcgcgcggct
     1921 tccgcgccgc cgccgaacac ctcgccgagc agctccgcgc ctacggcctc gccgacgcgc
     1981 ggatcgagca gttccccgcc gacggcaaga ttttctacgg cacgcagcgc tcgcgcccgc
     2041 cgtgggacgc cgatttcgcc gagttgtggg aggtgcgcgg cgacggactc gtttcgacgc
     2101 cgacccgctg gctgccggtc gcgcggctgg cgagctggga ggcgatgccc gtgacgctcg
     2161 cgcaggacag cgagagcgcc gacgtgacgg ttgaacttgt ggacgtgggc ggcggcgtta
     2221 ccgagcggga ttacgcgtcg agggacgtgc gcgggaagat cgtgctggcg gcctcgcagc
     2281 ccggcccggt ggcgcggctc gcggtcgaaa aatacggcgc gctcggcatc gtcagctacg
     2341 cgcagaacca gcgcacggcg tggtggggcg agaacgaccg tctcgtccgc tggggccacc
     2401 tcgacacgtt cgccgcgaag ccgaccttcg ccttcatggt ctcgctcaag caggcgcgcg
     2461 agctacaggg ccgcatggcg cgcgccgagc gcgtctggct gcgggccgtg gtgcgcgccg
     2521 gtaaacacgc gggcgcgtac gacgtgctga cggcgaccat ccccggcgcc gacccgcgcc
     2581 tcaaggacga ggagatcgcc ttcagttgtc acctcgacca ccagcggccc ggcgcgaacg
     2641 acaacgcctc gggcagcgtc gccatcctcg aagtcgcgcg cacgctctcg aagctcgtcc
     2701 gcgaggggcg catccctccc cccgcgcgca ccatccgctt catctggccg ccggagatag
     2761 agggcacgct cgcgctcctc aacgcgcggc ccgacatcgc cgcgcggacg aaggccgtca
     2821 tccacatgga catggtcggc ggcggccccg aaacgaaatc cgtcttccac gtcacgcgca
     2881 gcccggcttc tctgccctcc ttcgtcaacg acgtggccga gcacttcggc gagttcgtca
     2941 acgaacagtc ggcgcgcttc gcggggggcg agagcgtccc ttactcgctg cactcgcccg
     3001 agggcggcaa ggaggcgctg ctcgcagaga tggccgagtt ctcttccggc agcgaccacc
     3061 aaatctacac agacagctcg tggggcatcc ccgccatcta tcttaacgac tggcccgacc
     3121 gctacatcca cacgaacttc gacacgcccg ccaacgtcga tccgaccaag ctcaagcgcg
     3181 ccgccttcat cggggccgcc agcggctact tcctcgccga cacgaaggcg gacgacgccc
     3241 cagccctgct acgcctgttc cggtctcaag ccctgcgccg cacggcccgc atgctcacac
     3301 gccgcgattc gctcccgccc gctgaggccg ctaacctcac gcgctttcac cggtggtatg
     3361 aacgtgccgt tctcgcttca atgagccgct tcttcaaagt ccccgaatct gtaggcgcca
     3421 cgggggcgta ctttctcgac accttgcaga caatcactgg cccgccttcc cctctccctc
     3481 cgacgccagc gggcgacggg cgggtcgtct tccgccgcaa cgcgaagttg aaagggccga
     3541 tgtcggcctt cggctacgac tacttcacgg acaaactcgg cgaggagcgc gtgcggcagg
     3601 tgcgcctgct gcgcttcgag ggcgcacgcg gctcgggcgg cgactacgct tacgaagtcc
     3661 ttaacttcgt ggacgggcgg cgcaccgcgc aggagatacg cgacgcggtc tccgccgtct
     3721 acggcccggt gccgctcgaa acggtcgtcg aatacctgcg cgcgctcgaa accatcggcg
     3781 cggtcgagtc gaaccgctga acgggggccg ccgggtgaga cgggtttcga ttgcggcgcg
     3841 gatgtggtaa aaggtctcga ctgccggagt
//
LOCUS       DBNGOH010000073         4912 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_194316, whole genome shotgun sequence.
ACCESSION   DBNGOH010000073 DBNGOH010000000
VERSION     DBNGOH010000073.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4912)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4912)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4912
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_194316"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(193..873)
                     /locus_tag="AC159P_01385"
     CDS             complement(193..873)
                     /locus_tag="AC159P_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016779462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-dependent dioxygenase"
                     /protein_id="HIR3694131.1"
                     /translation="MLLHIPGVLSAEQVARCRHVLGETEWVDGRVTAGHQSARAKNNM
                     QLPEHHTAARELGDMIVAALERNPLFMAAALPSKVFPPLFNRYEGGQSFGNHVDNAVR
                     QVTGTPHRVRTDLSVTLFLAQPEEYDGGELLVEDTYGVHGVKLPAGDLILYPASSLHR
                     VNPVTRGTRLASFFWVQSMVRDDGERALLFDLDTSIQRLVQDSPEHPSAMQLTNVYHN
                     LLRRWADV"
     gene            complement(896..3364)
                     /locus_tag="AC159P_01390"
     CDS             complement(896..3364)
                     /locus_tag="AC159P_01390"
                     /inference="COORDINATES: protein motif:HMM:TIGR01783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015343 - siderophore-iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HIR3694132.1"
                     /translation="MKTDKHVQKRRKKGRRGPRYWVLSIGTMGALVAFTVGNSRAVAL
                     GYARHAEAVVTAADAQGQNRSQVLRFDIPPGTLETVLDAFQSLTGLRVMIPNDAMRGL
                     ASPGVSGAHTPEQALKQLLAGTGVAYRFTGAETLTLEIQAAPESVEVIDRTSPLSSPK
                     YTEPLRDIPQTITVIPKEVIEEQGATTLRDVLRNVPGLTMTAGEGGAPAGDNLTLRGF
                     SARNDIFVDGVRDLGPQSRDPFNLEQVEVVKGPGSAYTGRGSAGGTINLTSKAPGLTP
                     SYGGTLNLGNARMKRATADINAPLAKLGFGERTAFRLNLLAHDAGVAGRDVVEEERWG
                     VAPSLAFSLGGRTRLTLSYFHLQQDNVSDYGIPWVPATNNALAEFRDRPAPVPRDTFY
                     GFKDRDHEKLRSDLATFRLEHDFDDRLRLTNQLRYGRSTRDSMATPPRFADVNSTVIN
                     REMRSWLTEDDIWDNQTDMRASFETGAVRHTLVTGLALTRERNVRRTRTAANTQTTLL
                     DPNPNDVFTGAILLNPFVGDITADSQALYAFDTARFGERWEFNGGLRWDRFDAEGVTV
                     GTTSATGVFTPGTPVARVDRMLSWRAGAVFKPRPEGSVYAAYGTSLSPSLEGLSYGTA
                     NTAIEPEKTYTFEAGSKWEMFGSRLLLTGAVFRVEKTNARTPGLPGEPPQVLDGEQRV
                     DGVELSASGRVVRGWQLFAAYTFLDSEIVRSNTATEVGKEILNTPRNSFSLWTTYTSP
                     WMLEVGGGARFVGRRFGNNTNTRQVEGYWLLDLMASYPLSRHVDLRLNINNLTDAYYF
                     DRIGGGHLVPGAARTVMLSTGFRF"
     gene            4104..4685
                     /locus_tag="AC159P_01395"
     CDS             4104..4685
                     /locus_tag="AC159P_01395"
                     /inference="COORDINATES: protein motif:HMM:NF012973.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
                     /protein_id="HIR3694133.1"
                     /translation="MSEKWIPARAAAAAVGRGLTTWDAHEFLVLALSAVLRRKAADFI
                     GLDEARALLARIEPVFPQLVAETVPKTVSLFGLTDVLRRLVVELVFVRNLRRILLALA
                     DWGRVENDPLLLTEYVRAALRRQLTYRLSRGTNQLVEFLLHPEIETAIRDATRHTATG
                     SYVELEPPACERSWTPSVSRCARCRTAYRFPRF"
     gene            4691..4867
                     /locus_tag="AC159P_01400"
     CDS             4691..4867
                     /locus_tag="AC159P_01400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694134.1"
                     /translation="MEIRSSVRRLVSTSLPWLHVVSYQELRPDTSIQPVGRVSLDGFS
                     PRPGVSVCGVPLWG"
BASE COUNT          801 a         1623 c         1623 g          865 t
ORIGIN      
        1 gacatgatga gcaccaccac cccggcgaag acgcccgccg ccaaatggca ccagaagatg
       61 actttgcgaa gtagcttcat cagtaacaaa tccctgcggg agaatcggtc acgagtaaat
      121 atgcagcgac ccgctgacac gtgggccgcg aaagcgacct tcgcacggcg cgcgcggcta
      181 cttgaacacg cctcacacgt ccgcccagcg gcgcagcagg ttgtgataga cgttggtgag
      241 ttgcatcgcc gaggggtgtt cgggagagtc ttgaacgaga cgctgaatcg aggtgtcgag
      301 gtcgaagagg agcgcccgct cgccgtcgtc gcgcaccatg ctctgcaccc agaagaagga
      361 cgcgaggcgc gtgcctcgcg tcacagggtt gacacggtgc aggctgctcg cagggtaaag
      421 aatcaagtcc cctgcgggca gcttcacgcc gtgcacgccg taggtgtctt cgaccagcaa
      481 ctcgccgccg tcgtactcct cgggctgcgc gaggaagagc gtgacggaca agtctgtacg
      541 gacgcggtgc ggcgtgcccg tcacctgccg gacggcgttg tccacgtggt tgccgaaaga
      601 ctggccgccc tcgtagcggt tgaagagggg agggaagacc ttcgacggca gcgccgccgc
      661 catgaagagc gggttgcgtt cgagcgccgc gacgatcatg tcgcccaact cccgcgctgc
      721 cgtgtggtgc tcgggcaact gcatgttgtt tttggcgcgc gccgactggt ggcccgcggt
      781 gacgcgcccg tccacccact cggtctctcc gagcacgtgc ctgcaacgtg cgacctgttc
      841 ggcgctcagc actccgggga tgtgtaacag catcttcttt cgtcaaagcc ccgcgtcaaa
      901 agcggaagcc cgtgctgagc atcaccgtgc gcgccgcgcc ggggacgagg tggccgccgc
      961 cgatgcggtc gaagtaataa gcgtcggtca ggttgttgat gttgagccgc aaatcgacgt
     1021 ggcgcgagag cgggtaggag gccatcaggt cgagcagcca gtagccttcc acctgccgcg
     1081 tgttggtgtt gttgccgaag cggcgcccga caaagcgcgc gccgccgccg acttccaaca
     1141 tccatggcga ggtgtaggtc gtccacaggc tgaaggagtt gcgcggcgtg ttgagaatct
     1201 ccttgccgac ctccgtcgcg gtgttcgacc tgacgatctc gctgtcgagg aaggtgtagg
     1261 cggcgaagag ttgccagccg cgcacgacgc ggcccgacgc gctcagctcg acgccgtcca
     1321 cgcgctgctc gccgtcgagc acctgcggtg gctcgcccgg cagccccggc gtgcgcgcgt
     1381 tggtcttctc gacgcggaag accgcgcccg tcagcagcag gcggctcccg aacatctccc
     1441 acttgctgcc cgcctcgaag gtgtaggtct tctccggctc gatggctgtg ttggccgtcc
     1501 cgtaagacag cccttcgagc gaggggctga gcgaagtgcc gtaggcggcg tagacgctcc
     1561 cctccgggcg cggcttgaag accgcgcccg cgcgccaact gagcatccgg tcaacgcgcg
     1621 cgaccggcgt gcccggcgtg aacacgcccg tcgccgaagt cgtcccgacc gtgacgcctt
     1681 cggcgtcgaa acgatcccag cgcagcccgc cgttgaactc ccacctctcg ccgaagcgcg
     1741 cggtgtcgaa ggcgtagagc gcttgcgagt ccgccgtgat gtcgccgacg aatgggttca
     1801 gcaggatcgc gcccgtgaag acatcgttcg ggttggggtc gagcagcgtg gtctgcgtgt
     1861 tggcggcggt gcgcgtgcgg cggacgttgc gctcgcgggt gagcgcaagg ccggtgacga
     1921 gcgtgtgcct gaccgcgccg gtctcgaagc tcgcgcgcat gtccgtctgg ttgtcccaga
     1981 tgtcgtcctc cgtcagccac gaacgcatct cgcggttgat gacggtcgag ttgacatcgg
     2041 cgaagcgggg cggcgtcgcc atcgagtcgc gcgtcgagcg cccgtagcgg agctgattcg
     2101 tgagtcgcag acggtcgtcg aagtcgtgct caagtctgaa ggtcgcgagg tcggagcgca
     2161 gcttctcgtg atcccgatcc ttgaagccgt agaaggtgtc gcgcgggacg ggcgcggggc
     2221 ggtcgcggaa ctccgcgagg gcgttgttgg tggccggcac ccaggggatg ccgtagtccg
     2281 agacgttgtc ctgttgcagg tggaagtagc tgagcgtgag gcgcgtccgc ccgccgaggc
     2341 tgaaggccag cgacggcgcg acgccccaac gctcctcttc cacaacgtcg cgcccggcca
     2401 cgcctgcgtc gtgggccagc aggttcaggc ggaaggccgt gcgctcgccg aagcccagct
     2461 tggcaagcgg cgcgttgatg tcggcggtgg cgcgcttcat ccgcgcgtta ccgaggttga
     2521 gcgtgccgcc gtaggacggc gtgaggcccg gcgccttgct cgtcaggttg atggtgccgc
     2581 ccgccgagcc gcgccccgtg taagccgacc ccggcccctt caccacctcg acctgttcga
     2641 ggttgaacgg gtcgcgcgac tgcgggccga ggtcgcgcac gccgtcaacg aagatgtcgt
     2701 tgcgggcgct gaagccgcgc agcgtgaggt tgtctcccgc gggcgcgccg ccctcgcccg
     2761 cggtcatcgt cagaccgggg acgttgcgga gcacgtcgcg cagcgtcgtc gcgccctgct
     2821 cttcgatcac ctctttcggg atgaccgtga tcgtctgcgg gatgtcgcgc agcggctccg
     2881 tgtacttcgg cgatgaaagc ggggaagtgc ggtcgatcac ctcgacggac tcgggcgcgg
     2941 cctgtatctc cagcgtcaac gtctccgccc ccgtgaagcg gtaagcgacg cccgtgccgg
     3001 cgagaagttg tttgagcgcc tgctcgggcg tgtgcgcgcc cgacacgccc ggtgacgcga
     3061 ggccgcgcat cgcgtcgttc ggaatcatca cacgcaggcc ggtgagactc tgaaacgcgt
     3121 ccagcaccgt ctccagtgtg cccggtggga tgtcgaaacg caggacttga gatcgattct
     3181 gtccctgcgc gtccgcggcg gtcacgacgg cctccgcgtg gcgcgcgtac ccgagcgcga
     3241 cggcgcggct attcccaacc gtgaaggcca ccagcgcgcc catcgtgccg atgctcagca
     3301 cccagtatct aggcccgcgc cgacccttct ttctcctctt ctgcacgtgc ttatccgtct
     3361 tcatctccac cgccttcctt cgaacatcga cagcgctgaa cgggggccgc gccgcgtcca
     3421 ccattcggac acaggaaacg cgcctcccct ccgcacacgt agctttccgg actcacgcga
     3481 ccgcggacga gcattaacaa aagggatgac gccgctcacg agacttcgac cggcggcact
     3541 gtcaccgtcc tcgcttaaac gggacggcga caccgcccga cgtaatcagg tcaaccgcct
     3601 gcgattgatg tgtagataat cgagcgtccg tcaggaaggc atgctaggat agcaatgcac
     3661 accatccgtg aaacctctcc aagggcatag cgaatcggtc gtgcacagag gctttcagcg
     3721 tggccgcgcc gaaagcctcg attttttaca cacctgaagg ctctgaaatt gaaaatcgtt
     3781 ttcaaaagca ggcgtaatat atgttagtgc ctcggcattg tcaaatcatt tttgactgac
     3841 ggattgttgg tagtcgcccc attacgtttg ggattgaaaa ctgaagggcg gagtgtggac
     3901 agtgtcgggt gtcgtgagcc gacgcagaat ccactaccct ccgccctcct tgcgctgccc
     3961 tcattgtaga agcagcgcac gccacttgtt ctatcacagc acctatcgca cccgcgacgg
     4021 ccctcggctc ttgtggcaat gccggaagtg ccatcggtgc ttagcagagc ggcgcgggac
     4081 ggccttcttc aacctcaaga cccctgagcg agaaatggat tccggcccgc gcggcggcgg
     4141 cagccgttgg ccgcggcctc acgacgtggg acgcgcacga gtttctcgtc ctcgcgctct
     4201 cggcggtgct gcggcgcaag gcggctgact tcatcggact cgacgaggcg cgagcgctgc
     4261 tggcgcgcat cgagccggtc ttcccgcaat tggtcgccga gaccgtgccg aagaccgtgt
     4321 cgctgttcgg gctgaccgac gtgctgcggc gtctggtcgt cgagcttgtc tttgtccgca
     4381 acctgcgccg catcctgctg gcactcgccg attgggggcg cgtcgagaac gacccgctcc
     4441 tgctgaccga gtacgtccgc gccgcgttgc gtcgccagct cacgtaccgg ttgagccgcg
     4501 gcacgaacca gctcgtcgag tttctgcttc accccgagat cgagaccgcc atccgcgacg
     4561 ccacacggca cacggcgacg ggctcctacg ttgaactcga accgcccgcc tgcgaaagat
     4621 cgtggacgcc atccgtgagc cggtgtgcgc gctgccggac ggcgtacagg ttccccagat
     4681 tctgaccctc atggaaatcc gctcttccgt ccgccgtctg gtgagcacgt cgctgccctg
     4741 gctccacgtc gtgtcctatc aggagctgag gccggacact tccatccagc cggtcggacg
     4801 cgtctcgctc gacggcttca gcccgcgccc cggtgtcagc gtctgcggtg tcccgctgtg
     4861 gggctgaccg agtcggcccc cgcaccgggc gcgcaatttg gccggcaaca gc
//
LOCUS       DBNGOH010000074         6013 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3677276, whole genome shotgun sequence.
ACCESSION   DBNGOH010000074 DBNGOH010000000
VERSION     DBNGOH010000074.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6013)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6013)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6013
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3677276"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(125..247)
                     /locus_tag="AC159P_01405"
     CDS             complement(125..247)
                     /locus_tag="AC159P_01405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694135.1"
                     /translation="MASGANKQGTIARRVAETAGRVVGTVAGVIGSLTGARKKR"
     gene            complement(240..1352)
                     /locus_tag="AC159P_01410"
     CDS             complement(240..1352)
                     /locus_tag="AC159P_01410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694136.1"
                     /translation="MSRERDEDRDEVFGGDADTDETVRAGGDIDAASTSGGTGEPGGE
                     PADGKPMYLDHPSPPAADVAAEDGAEGARSGEGGHGGAAIGRTTGMGAGVTAGGTPGG
                     GEGTAGEGTAGGGAAGGGTANRGASSRAGAGGTSKTGGASGGGAGGASRGGAAKGAAG
                     GGPSQGSGGAAKKGAGTGGGAARGAGASATKGASGGSAAKGAGALKGGGALKGAGASK
                     GGAAAKGGASKGGGVSKGGGVSKGAGAAKGAGPSKGGGAGKGGASKGGAKKGGMTKAA
                     AKKGGGVSKGGAKKPGKGGPSYGSTGKGASKGGGRASAARGGSKSLTKGGGAKAATGR
                     SGATSARGGSGRGTTAGGGTSKKGGAGKGGSRKGGG"
     gene            1595..2152
                     /locus_tag="AC159P_01415"
     CDS             1595..2152
                     /locus_tag="AC159P_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011529777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2721 domain-containing protein"
                     /protein_id="HIR3694137.1"
                     /translation="MDALSSALTVLTAMITPAVLISACGTLILSTSTRLGRVVDRVRS
                     LSDSFEELAHSEGTLELFEERRAVIFDQLDKLTSRARILQRSLTVFYTATGIFVAASV
                     AIGVTAAIDGTRYAWVPVVLGLIGALFLFYGSILLIFEARLALSTLSAEMDFIWRLGK
                     TLAPAELVDQRSPHFNRLTLKRRRR"
     gene            2149..2439
                     /locus_tag="AC159P_01420"
     CDS             2149..2439
                     /locus_tag="AC159P_01420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694138.1"
                     /translation="MTTAASYLLLHETGELARRVEALERLLAACTVCPLDCGNNRLND
                     ELARCFSGRLPVVSSYTPHFGEEPALAGTRGAGNVFFGNCNSKNNPFSYNNL"
     gene            complement(2615..2974)
                     /locus_tag="AC159P_01425"
     CDS             complement(2615..2974)
                     /locus_tag="AC159P_01425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HIR3694139.1"
                     /translation="MQARLYIDEDAMDDDLVQALRVRGADVQTAFEAGLVGIPDEEHL
                     AHTAEEDESSTPSTSATTLRLHGEDMNEGRAHAGIVFDDRQRFSVGEQMRRPLRIISL
                     RGAEALRGGYEFLSAWK"
     gene            complement(2952..3206)
                     /locus_tag="AC159P_01430"
     CDS             complement(2952..3206)
                     /locus_tag="AC159P_01430"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3694140.1"
                     /translation="MRASSARSSKTQTWPSSSPITIGDPRRRGRGLGRFLLEAAQEFR
                     RSRGVPREVLSTAARNEAAQRLFAGAGFRQTSPSAGTPLH"
     gene            3311..>6013
                     /locus_tag="AC159P_01435"
     CDS             3311..>6013
                     /locus_tag="AC159P_01435"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF020044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIR3694141.1"
                     /translation="MSNNDRRFVGAGREAQGVWSPATLSVERLIARAGATIAARGRAY
                     YAQKWRLRVQRVSAEEAVVRVRGSRQYTVEFMRDGGRLLVFCDCPYADYEEDVICKHG
                     VAAALFLRDHLQQDAPVPWQDVLSQAVRTSSAPASKGPAEFLFFSLQARGNSWAVMPY
                     TVAAPFFPETARQDAAEAARVVSEQRLSSQAEKVSPYGSGGPRVYVNVTDAQAALVKM
                     LASSGLHYGSYYGGAALDIGALLALLKGGPLFLGTDKNPLKRPLEVCDDVARIELEME
                     RTDEGGTLLSALAVAGERVFPLRERGMQAVSYRPLWLLSGQTVFQLDAPPEGFALFHE
                     SPAIHIPGDEEQAFFEKHLPYLAERFPLRGAAVRWEELRDVEPVPRLYLTEEGKELRA
                     ELRFGYGGHEVPYSRSAPAESIRRDAQTGALVRIARWPERELEWQETLISKTYGLKRG
                     SGDYGSPRNFEATFFYLRANTHPLDFLLRHVPQLAAAGCEIYGEEELKLARINRHRPT
                     ISFGVTSGIDWFDVQAVVRFGDVEVALAEVRRAVRKRERFVKLADGSVGEIPPEWIEQ
                     YRHLFALGEEADGGLRLAHHHLTLLDQLLADDTRARTDKEFKRRRERLRNFDGIKSQK
                     LPRNFDGELRPYQKAGIDWLYFLREYRFGGCLADDMGTGKSVVTLAFLQSLRERGLAK
                     QATLLVVPRSLVFNWEREAARFTPRLRLLNHATASRTRDVSDFDDYDVVLTTYALLRR
                     EIEMLMHYEFNYLILDEAQAIKNPLAETAKAARLLRGASRLTLTGTPVENNTLELWSQ
                     FAFLNPGLLGNAEYFREEFATPIERKGDERAAQFLRKMVYPFILRRTKDQVAKDLPPR
                     TERQMVCDMEPAQRKLYDERRAYYRALLLKLIEDEGINNARFRVL"
BASE COUNT          921 a         2145 c         1889 g         1058 t
ORIGIN      
        1 atgcgaggtg atgaacgggc cgcgccggat gaagagcgat aagccgtgaa ccgtgaaccg
       61 taaaccgtga caaggaacgg actgcttccc cttgtcacgg tttacggttg acggttcacg
      121 gcctttaacg cttcttgcgc gcgccggtca gagagccgat gacccctgcg accgtgccga
      181 cgacgcggcc cgcggtctcc gcgacgcggc gggcgatagt gccttgtttg ttagcgccgc
      241 tagccacctc ctttgcgtga gccgcctttg ccggcgccgc cttttttgct cgtgccgccg
      301 ccggctgtcg tgccgcgtcc gctgccgccg cgcgcggagg tcgcgcccga acgaccggtc
      361 gcggccttcg cgccgccgcc cttcgtcaag ctcttcgagc cgccgcgcgc cgccgaagcg
      421 cgcccgcccc ccttcgaagc gcccttgccc gtcgagccgt aactcggccc gcccttgccg
      481 ggtttcttcg cgccgccctt gctcacgccg ccgcctttct tcgcggcggc cttcgtcatg
      541 ccgcctttct tcgcgccgcc cttcgacgcg ccgcccttac ctgcgcctcc gcctttggaa
      601 ggcccggcac ctttagcggc acccgcgccc ttcgacacgc cgccgccttt ggaaacgccg
      661 ccgcctttcg atgcgccgcc tttcgcagca gcgccaccct tacttgcgcc cgcgcccttc
      721 aaagcgccgc cgcctttcaa agcgcccgcg cccttcgcgg cagaaccacc gctcgcgcct
      781 ttcgtcgcac tcgcgcccgc accgcgcgcc gcgccgccgc ccgtgccggc tcctttcttc
      841 gccgcgccgc cgcttccttg actcgggccg ccgcctgcgg cccctttcgc cgcgcctccc
      901 ctactcgcgc ctccggcgcc gccgccgctc gcgccgccgg tcttcgacgt gccgcccgcg
      961 cccgcgcgcg acgaagcgcc tctgttcgca gtgccgccgc ccgcagcgcc gccgcccgcg
     1021 gtgccttcac ccgcagtgcc ttcgccgccg ccgggcgtgc cgcccgcggt gacgcccgcg
     1081 cccatgcccg tcgtgcgacc gatggccgcg ccgccgtgtc cgccctcgcc ggagcgagcg
     1141 ccctcggcgc cgtcctctgc ggcaacatcc gccgcggggg gactcggatg gtcgaggtac
     1201 atgggcttgc cgtccgcggg ttcgccgccc ggctcgccgg tgcctccgct ggtgctcgcg
     1261 gcgtcgatgt cgccgcccgc gcgcacggtc tcgtcggtgt cggcgtcgcc gccgaacacc
     1321 tcatccctgt cttcgtctct ttccctggac atctcctgct ctcctttgcg agaaaatgga
     1381 gacccctgtc tcacccgaga cggagtcttc tcaatgtggc ttgtagttgc gaactgcttt
     1441 gcggcggagg atagcacaac ttcaagagag gcgagccaca cttttcatcc cccggtcttc
     1501 gttttttcgc cgcgcacgcg gcggctggcg caggcgcgcc gcaggcgata taaatagcaa
     1561 gcgtcggaca cttccccggc ttgcggaaag aacgatggat gcactctctt cggccctgac
     1621 cgtgctgacg gcgatgatca cgcccgccgt gctcatctct gcctgcggca cgctcatcct
     1681 ctcgacctcg acccgtctgg gccgagtcgt tgaccgcgtc cgctcgctct ccgacagctt
     1741 cgaagaactg gcgcacagcg aaggcacgct cgaactgttc gaagagcgcc gcgccgtcat
     1801 cttcgaccag ctcgacaagc tgacgagccg cgcgcgcatc ctccaacgct cgctcaccgt
     1861 cttctacacg gcgaccggca tcttcgtcgc ggcgagcgtc gccatcggcg tcaccgccgc
     1921 catcgacggc acgcgctacg cgtgggtgcc ggtcgtgctc ggcctcatcg gcgccttgtt
     1981 cctcttctac ggcagcatcc tcctcatctt cgaggcacgg ctcgcgctct cgactttaag
     2041 cgccgagatg gacttcatct ggcgcctcgg caagacgctc gcgcccgccg agctggtcga
     2101 tcagcgcagc ccccacttca accgcctcac cctcaaacgg agacgaagat gacgaccgcc
     2161 gcctcctacc tcttgctcca cgagacgggc gagctcgcgc gccgcgtcga ggctctggag
     2221 cgactgctcg cggcctgcac cgtctgcccg ctcgactgcg gcaataatcg tttgaacgac
     2281 gaactcgcgc gctgcttctc cgggcggctc ccggtcgtct cctcctacac gccgcacttc
     2341 ggcgaggaac ccgcgctcgc gggcacgcgc ggcgccggca acgtcttctt cggcaactgc
     2401 aatagcaaaa acaacccgtt ttcttacaac aacttataag gcgagacttt aagccttgac
     2461 ggcttttgac ggctgtggag ctaacaattt cgagcatcct acgacgtatt aatttacggg
     2521 tcaagcatca gagagattcg ataaccgccg agactgctcc tgcttagtag ccccttcatt
     2581 catacgtcct caacattagt tgtgaggtcg gtgactactt ccaagcgctg aggaactcgt
     2641 agccgccgcg cagcgcttcg gcaccgcgga gtgagatgat cctcagcgga cgccgcatct
     2701 gctcgcccac gctgaagcgc tgccggtcgt cgaagacgat gccggcgtgc gcccgacctt
     2761 cattcatgtc ctcgccgtgg aggcgcaatg tagtcgccga cgttgaaggt gtagaggact
     2821 cgtcctcttc tgccgtgtgg gcgaggtgct cttcgtcggg tatcccaacc agccccgcct
     2881 cgaacgcggt ctgaacgtcg gccccgcgca cgcggagcgc ctgcacgagg tcgtcgtcca
     2941 tcgcgtcctc gtcaatgtag aggcgtgcct gcactaggcg acgtctggcg gaaccccgcg
     3001 cccgcgaaga gccgctgcgc cgcctcattc cgcgcggccg tagacagcac ctcgcgcggc
     3061 acgccgcgcg acctgcggaa ctcctgtgcg gcttcgagga gaaatcggcc caagccccgg
     3121 ccgcggcgcc tcgggtcgcc gattgtgatc ggcgacgagg acggccacgt ctgggtcttc
     3181 gagctacgtg ccgatgaagc acgcatatat aggtctgcga caagctcgcc cttcggctgg
     3241 aagtgtaaac tgttctcgcg gtagctatac cagacgggta gattcgacgt agcatcatcg
     3301 aaaattaggc atgagtaata atgaccgaag atttgtcggc gcagggcgtg aagcgcaagg
     3361 ggtctggtct cccgccacgt tgagcgtcga gcgcctgatc gcgcgtgcgg gcgcgaccat
     3421 cgccgcgcgt ggccgcgcct actacgcgca gaaatggcgc ctccgcgtgc agcgcgtgag
     3481 cgccgaggaa gccgtcgtca gggtgcgggg cagccggcag tacacggtcg aatttatgag
     3541 ggacgggggg cgtctgctcg tgttctgcga ctgcccgtat gccgactacg aggaggacgt
     3601 aatctgcaag cacggcgtcg cggcggcgct gtttctcaga gaccacctcc aacaggacgc
     3661 gccggtgccg tggcaggatg tcctgtcgca ggcggtacgc acgtcttcgg cccccgcgtc
     3721 gaagggcccc gcggagtttc tcttcttcag ccttcaggcg cgcggcaact cgtgggcggt
     3781 catgccgtat acggtcgccg ccccgttctt cccggagacg gcgcggcagg acgcggcgga
     3841 ggcggcccgc gtggtctcgg agcagcggct ctcgtcgcag gcggagaagg tcagcccgta
     3901 cgggtcgggc gggccgcgcg tctacgtcaa cgtcacagac gcgcaggcgg cgctggtgaa
     3961 gatgctggcc tcgtccggcc tccactacgg cagttattac ggcggcgcgg cgctcgacat
     4021 tggcgcgctg ctcgcgcttc tgaaaggcgg gccgctcttt ctcggcacgg acaagaatcc
     4081 gctgaagagg ccgctcgaag tatgcgacga cgtggcccgc atcgaactgg agatggagcg
     4141 gacggatgag ggtggcacgc ttctcagcgc gctggccgtc gcgggcgagc gcgtgtttcc
     4201 gctccgcgag cgcgggatgc aggcggtctc ttacaggccg ctctggctgc tctccgggca
     4261 gacggtcttt caactggacg cgccgcccga aggcttcgcg ctcttccacg agagccccgc
     4321 aatacacatc cccggcgacg aggagcaggc tttcttcgag aagcacctgc catacctcgc
     4381 cgaacgcttc cccctgcgcg gcgcggcggt tcgctgggaa gagttgcgcg atgtcgagcc
     4441 cgtgccgcgg ctctacctga ccgaagaggg gaaggagttg cgcgccgagt tgcgtttcgg
     4501 ctacggcggc catgaggttc cgtacagccg cagcgcgccc gccgagagca tccgccgcga
     4561 cgcacagacc ggcgcgctcg tgcgcatcgc gcgctggccc gagcgtgaac tggaatggca
     4621 ggagacgctc atctcgaaga cctacgggct caaacgcggc tccggtgatt acggttcccc
     4681 gcgcaacttc gaagccacct tcttctactt acgcgccaac acccacccgc tcgatttcct
     4741 tctgcgccac gtgccgcagt tggccgccgc cgggtgtgag atttacggcg aagaggagtt
     4801 gaagctggcg cgcatcaacc gccaccgccc gaccatctcc ttcggcgtca cttcgggaat
     4861 cgactggttc gacgtgcagg cggtcgtccg attcggcgac gtggaggtcg cgctcgcgga
     4921 ggtgcgccgg gcggtgcgta agcgcgaacg cttcgtcaag ctggccgacg ggagcgtcgg
     4981 cgaaatcccg cccgaatgga tcgagcagta ccgccacctc ttcgcgctcg gcgaggaggc
     5041 ggacggaggg ctgcgcctcg cgcatcatca cctgacgttg ctcgaccaac tgctcgccga
     5101 cgacacgcgg gcgcgcaccg acaaggagtt taagcgccgc cgcgaacgcc tgcgtaattt
     5161 cgacggcatc aagtcgcaga agctccccag aaatttcgac ggcgagctgc gcccgtatca
     5221 gaaggccggg atcgactggc tctacttcct gcgcgagtac aggttcggcg gctgcctcgc
     5281 cgacgacatg gggacgggga agagcgtggt gacgctcgcc ttcctccaat cgctcaggga
     5341 gcgggggctg gcgaagcagg cgacgctgct ggtcgtgccg cgctcgctcg tcttcaactg
     5401 ggagcgcgag gcggcgcgtt tcacgccgcg cctccgcctc ttaaaccacg ccaccgcgag
     5461 ccgcacgcgg gacgtttccg acttcgacga ctacgacgtg gtgctgacga cctacgccct
     5521 gctccggcgc gagatcgaga tgttgatgca ctatgagttc aactacctga ttctcgatga
     5581 ggcgcaggcg atcaagaatc cgctcgcgga gacggccaaa gccgcgcggc tcctgcgcgg
     5641 ggccagtcgc ctgactctga cgggcacgcc ggttgaaaac aacacgctgg agctgtggtc
     5701 gcagttcgcc ttcctgaatc cggggctatt gggcaacgcc gaatacttcc gcgaggagtt
     5761 cgccacgccc atcgagcgca agggcgacga gcgggcggcg cagttcctgc gcaagatggt
     5821 ctaccccttc atcctgcggc gcaccaaaga tcaggtcgcg aaggacttgc ccccgcgcac
     5881 cgagcgccag atggtctgcg acatggagcc ggcccagagg aagctctacg acgagcggcg
     5941 cgcctactac cgcgcgctcc tgctcaagct gattgaggat gagggcatca acaacgctcg
     6001 cttccgcgtg ctc
//
LOCUS       DBNGOH010000075         2481 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3860941, whole genome shotgun sequence.
ACCESSION   DBNGOH010000075 DBNGOH010000000
VERSION     DBNGOH010000075.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2481)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2481)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2481
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3860941"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..278
                     /locus_tag="AC159P_01440"
     CDS             <1..278
                     /locus_tag="AC159P_01440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694142.1"
                     /translation="SAELFAEAFLRGDVASYDEAWRGDFGRELRRASQLRRRFYGQFW
                     GGPFTDRMIRFARAHRGIRRTLRELVAGDQSYLDLKRTLMRRAAWPL"
     gene            complement(471..911)
                     /locus_tag="AC159P_01445"
     CDS             complement(471..911)
                     /locus_tag="AC159P_01445"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HIR3694143.1"
                     /translation="MSKPWKYRIEERVRWGDVDAARIIFYGAYIRFFEFAETELFRAV
                     GLPYGVMFDELDVWLPRAHLECDFRRAARLDDLLEVCVYVGRVGTKSLRLDFEVRRVG
                     EAELIADAHFVLVAVRRDTFDSVPVPEELKRRLAPYTQPPATDT"
     gene            complement(908..1114)
                     /locus_tag="AC159P_01450"
     CDS             complement(908..1114)
                     /locus_tag="AC159P_01450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR3694144.1"
                     /translation="MPTQIVVGSEDAITPPKDSETMHALISGSLMKVIAGAGHVSNVE
                     RPAEFDRALAEFLAGLPPEQEATA"
     gene            complement(1121..1729)
                     /locus_tag="AC159P_01455"
     CDS             complement(1121..1729)
                     /locus_tag="AC159P_01455"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR3694145.1"
                     /translation="MSKMARVRGIEMAYDDSGGAGAPVVLLHGFPFNRSLWNEQAAAL
                     GAAHRVVTPDLRGHGETTVTGGQATMEEMAEDVAALLDELGLKRVVLGGLSMGGYVTL
                     AFYRLFPERVRALVLADTRPQGDDDKARQTREETAQRASREGMGVVVESMLPKMLAPA
                     TFEGEPRVVSRVREMMLGTEPEGAAAALRGMAVRREQTALLE"
     gene            complement(1871..>2481)
                     /locus_tag="AC159P_01460"
     CDS             complement(1871..>2481)
                     /locus_tag="AC159P_01460"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR3694146.1"
                     /translation="GGFVRDAYRAETLRARAPLLVGTVGELSAVKGQDDFVRAASLVA
                     RKKEQAVEFLVVGEDHSRGGENRARLEALIDELGLGGRVRLLGRTEELARLLSSLDVF
                     VSASRSEAFGLAMVEAMACGCAVVSTATEGAREIVEDGATGVVVPVGDPPALAAAIES
                     LLDDAPLRNSLAERARESARTRFDLARMVEATERVYAEAVSS"
BASE COUNT          403 a          830 c          848 g          400 t
ORIGIN      
        1 gctcggcgga gctattcgcg gaggcgttcc tgcgcggcga cgtggcatct tacgacgagg
       61 cgtggcgcgg cgacttcggg cgtgagctgc gccgcgcctc gcagttgcgc cgcaggttct
      121 acggccagtt ctggggcggg ccgttcaccg acaggatgat tcgtttcgcg cgcgcgcacc
      181 gcggcatccg ccgcacgctc cgcgaactcg tcgcgggcga tcagagttac ctcgacctca
      241 aacgcacgct catgcgccgc gccgcgtggc ctctgtgaag aagtaggcag taggcagtta
      301 aaagctgaag cattgagagg cttgggacgt tatctcccaa gcctctgcgc ccttcttctt
      361 ttactgccta ctgtctaccg cctactgctt accggctttg gagcatggag cgcgcgcgtt
      421 cgagggagga gacgttgttg agtacgtcgt cgaggagggc gcggacgcga tcaagtgtcg
      481 gtcgcgggcg gctgcgtgta gggcgcgagg cggcgcttca attcttcggg gacggggacg
      541 gagtcgaacg tgtcgcggcg cacggcgacg aggacgaagt gcgcgtcggc gatcagctcc
      601 gcctcgccga cgcggcgcac ctcgaagtcg aggcgcaggg acttggtgcc gacgcgaccg
      661 acgtagacgc agacttccag caggtcgtcg aggcgcgcgg cgcggcggaa gtcgcattcg
      721 aggtgcgcgc gcgggagcca cacgtccagc tcgtcgaaca tcacgccgta gggcaggccg
      781 acggcgcgga acagctccgt ctcggcgaac tcgaagaagc ggatgtaggc gccgtagaag
      841 atgatgcgcg cggcgtccac gtcgccccag cgcacgcgct cctcgatgcg gtatttccac
      901 ggtttgctca cgccgtcgcc tcctgctcgg gcggaagacc tgcgagaaac tccgcgagcg
      961 cgcggtcgaa ctcggcgggc cgctcgacgt tcgagacgtg acccgcgccc gcgatgacct
     1021 tcatgagaga gccgctgatg agagcgtgca tcgtctcgga atctttgggc ggcgtgatgg
     1081 cgtcttcgct cccgacgacg atctgcgtcg gcactttaat ctattcgagt aaagctgtct
     1141 gttccctgcg cacggccatg ccgcggagcg ctgccgccgc gccttccggc tcggtgccga
     1201 gcatcatctc gcgcacgcgg ctgacgacgc gcggctcgcc ttcaaacgtc gcgggcgcga
     1261 gcatcttcgg gagcatggat tccacgacga cgcccatgcc ttctcgcgag gcgcgctgtg
     1321 cggtctcctc gcgcgtctgc ctggccttgt cgtcgtctcc ttgcgggcgt gtgtccgcga
     1381 ggacgagcgc gcgcacgcgt tcggggaaga ggcggtagaa ggcgagcgtc acgtagccgc
     1441 ccatcgaaag cccgccgagc accacacgct tcaagccgag ttcgtcgagc agcgcggcca
     1501 cgtcttcggc catctcttcc atcgtcgcct gtccgcccgt gacggtcgtc tcgccgtggc
     1561 cgcgcaggtc gggcgtgacg acgcggtgcg ctgcgccgag cgccgcagcc tgttcgttcc
     1621 acagcgaacg gttgaagggg aacccatgga gcaggacgac cggcgcgccc gcgcccccgc
     1681 tgtcgtcgta agccatctcg atgccgcgca cgcgcgccat tttactcatc atcagacctc
     1741 ggcaaaaaat cggacgttga cgttgaccga cgagacgaca ttctaacccg actgcgacgc
     1801 gggcacacga cgccgcgaag gagtaacgac agcggccctc tcaagaagga gaggaatgcc
     1861 ggcggccctc tcaagatgaa accgcctccg catagacgcg ctccgtcgct tcgaccatgc
     1921 gcgcgagatc gaaacgcgtg cgtgcagact cgcgcgctct ctcggcgaga gagtttcgaa
     1981 gcggcgcgtc gtcgagaaga ctttcgatcg cggcggccaa cgcgggcggg tcgccgacgg
     2041 gcacgacgac gccggtcgcg ccgtcttcga cgatctcgcg tgcgccttcg gtcgccgtcg
     2101 agacgaccgc gcagccgcag gccatcgctt cgaccatcgc cagcccgaag gcttcggagc
     2161 gcgaggcgga gacgaacacg tcgagcgagg agagcagccg cgcgagttct tcggtgcggc
     2221 ccaggagacg aacgcgcccg ccgaggccga gttcgtcgat caaagcttcg aggcgcgcgc
     2281 ggttctcgcc gccgcgcgag tggtcttcgc cgacgacgag gaactcgacc gcctgctctt
     2341 tctttcgcgc gacgagcgaa gcggcgcgga cgaaatcgtc ctgccctttc actgcgctca
     2401 actcgccgac cgtcccgacg aggagcggcg cgcgcgcgcg cagagtctcc gcgcggtagg
     2461 cgtcgcgcac gaagccgccg a
//
LOCUS       DBNGOH010000076         3499 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_194638, whole genome shotgun sequence.
ACCESSION   DBNGOH010000076 DBNGOH010000000
VERSION     DBNGOH010000076.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3499)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3499)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3499
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_194638"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1556
                     /locus_tag="AC159P_01465"
     CDS             <1..1556
                     /locus_tag="AC159P_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIR3694147.1"
                     /translation="KKALVKKSRKGGRAAAKSGARTFRNYVGGEWVASRSGETFENIN
                     PADTREVVGRFPLSTAEDMNAAVEAARAAADKWRRLPAPRRAEILFRLGEILMRGKDR
                     FAAEMTREMGKVLKETGGDVQEAIDCTYYTAGEGRRLHGYTTPAEMPDKFAMCVRQPV
                     GLCGLITPWNFPMAIPSWKLIPALVCGNTVVIKPAEDTPLSTYNLIKACEEAGIPAGV
                     VNMVTGHGETVGAALTGHPSVRLISFTGSTETGKRVASACAERNAICSLEMGGKNAII
                     VMDDADVDLAVEGAVWGAFGTSGQRCTASSRVIVHKKVYKKFTQQLVERAKNLKVGNG
                     LDPKTDMGPVINADAVHKILGYVEIGQKEDGAKLATGGKRLDKGEHAHGYFIEPTVFA
                     DVAPSMRVAQEEIFGPVTSVIPAGSLEEAIDIGNGVRYGLSASIYTQDVNRAFHAMNE
                     MYTGIFYVNSSTIGAEVHLPFGGTKGTGNGHREAGTQVLDIFTEWKSVYVDYSGKLQR
                     AQIDEVQFE"
     gene            1680..2150
                     /locus_tag="AC159P_01470"
     CDS             1680..2150
                     /locus_tag="AC159P_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase"
                     /protein_id="HIR3694148.1"
                     /translation="MVELREPYERRPIRFLEEWRCEGWRLKVYGIAYRREAPRAELIE
                     TAKRLAREKLPRPASGGGRYGVGFVGVHDGRGTNFVFAAWWADENELHYHVWAAPSGE
                     VEDLRYMNEAGPLACVWDLRVVGFEREAWVEEVLANAAGPDIEAYVNRRLNEDA"
     gene            2208..2690
                     /locus_tag="AC159P_01475"
     CDS             2208..2690
                     /locus_tag="AC159P_01475"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3694149.1"
                     /translation="MGEGFRVERVTKDNWQEAMALEIAPEQRAFVPSVMYSLAKAFVE
                     PDGKKYEPYVVYAGAAAVGFYMLSHTPGDETKFYFTNFLIGREHQGRGHGRAALVDCL
                     ESVKARFPACEEVYLAVHPENVVATRLYESLGFYKFGFVIDGEDGMRVNLKKIGEQKQ
                     "
     gene            2687..>3499
                     /gene="ald"
                     /locus_tag="AC159P_01480"
     CDS             2687..>3499
                     /gene="ald"
                     /locus_tag="AC159P_01480"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000286 - L-alanine dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0042853 - L-alanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HIR3694150.1"
                     /translation="MIVGLPKEIKDNEYRVGLTPAGVRALTDAGHQVNVERAAGEGSG
                     FEDALYERAGATIIESADDVWAGADMIVKVKEPIAPEYPRMKEGLLLFTYLHLAPDRE
                     QTKQLLERKVTGIAYETITDKRGTLPLLTPMSEVAGRMAVQVGATYLERMNGGRGVLL
                     GGVPGVPAARVVIIGGGVVGTNAAKIAVGMGAHVTIIDRDLDRLRELDDIFLSKISTL
                     ASSAYAIHGAISEADLIIGAVLVPGAAAPRLVTREMLKDVPNGSVIVDVAVDQ"
BASE COUNT          636 a         1082 c         1246 g          535 t
ORIGIN      
        1 cgaaaaaagc tctcgttaaa aaaagcagga agggcgggcg cgcggcggct aagtcgggcg
       61 cccgtacttt taggaactac gtcggcggcg agtgggtcgc gtcccgctcg ggcgagacgt
      121 tcgagaacat caacccggcg gacacgcgcg aggtcgtggg gcgctttccc ctttcgaccg
      181 ccgaggacat gaacgcggcg gtcgaggccg cgcgcgccgc cgccgacaag tggcggcggc
      241 tcccggcgcc gcgccgcgca gagatcctgt tccgcctcgg cgaaatcctg atgcgcggca
      301 aggatcgctt cgccgccgag atgacgcgcg agatgggcaa ggtcttgaag gagaccggcg
      361 gcgacgtgca ggaggccatc gactgcacct actacacggc gggcgaggga cggcgccttc
      421 acggctacac gacgcccgcc gagatgcccg acaagttcgc catgtgcgtg cgccagcccg
      481 tcggcctctg cggactcatc acgccgtgga acttcccgat ggcgatcccc tcttggaagc
      541 tcatccccgc gctggtgtgc ggcaacacgg tcgtcatcaa gcccgccgag gacacgccgc
      601 tctcgacata caatctaatc aaggcgtgcg aagaggcggg catccccgcg ggcgtcgtca
      661 acatggtgac gggccacggc gagacggtgg gcgcggcgct caccgggcac ccttccgtca
      721 gactcatctc gttcaccggc tcgaccgaga cgggcaaacg cgtcgcctcg gcctgcgccg
      781 agcgcaacgc catctgctct ctggagatgg gcggcaagaa cgccatcatc gtgatggacg
      841 acgccgacgt tgacctcgcc gtcgagggcg cggtgtgggg cgcgttcggc acgtcgggcc
      901 agcgctgcac ggcttcgtcg cgcgtcatcg tccacaaaaa agtctacaag aagttcacgc
      961 agcagctcgt cgagcgcgcg aagaacttga aggtcggcaa cggcctcgac cccaagaccg
     1021 acatggggcc ggtcatcaac gccgacgcgg tgcacaagat tctcggctac gtcgagatcg
     1081 gccagaagga ggacggcgcg aagctggcga cgggcggcaa acggctcgac aaaggcgagc
     1141 acgcgcacgg ctacttcatc gagccgaccg tcttcgccga tgtcgcgccc tcgatgcgcg
     1201 tcgcgcagga ggaaatcttc ggccccgtca cctcggtcat ccccgcgggg tcgctcgaag
     1261 aggccatcga catcggcaac ggcgtgcgct acggcctctc ggcctcgatc tacacgcagg
     1321 atgtgaaccg cgccttccac gcgatgaacg agatgtacac cggcatcttc tacgtcaact
     1381 cctcgaccat cggcgccgag gtgcacctgc cgttcggcgg caccaaaggc accggcaacg
     1441 gccaccgcga ggccggcacg caggtcctgg acatcttcac cgagtggaag tcggtctacg
     1501 tggactacag cggcaagttg cagcgcgcgc agatcgacga ggtgcaattc gaataaccgt
     1561 gaaccgtaaa ccgtaaaccg tgacaagtaa gaaaggactc atcttgtcac tcgtcactcg
     1621 ttacacgtca ctgctcttca ctcatcacgg tttacggttt acggttcacg tcctttccaa
     1681 tggttgagct gcgcgagcct tacgagaggc gcccgattcg ttttctcgaa gagtggcggt
     1741 gcgaggggtg gcggttgaag gtgtacggca tcgcgtaccg ccgcgaggcg ccgcgcgcgg
     1801 aattaatcga gacggcgaag cgtttggcgc gtgagaagtt gccgcggccc gcgtcgggcg
     1861 ggggccggta cggcgttggc ttcgtcggcg tgcacgacgg gcggggcacg aacttcgtgt
     1921 tcgcggcgtg gtgggccgac gagaacgagc tgcactacca cgtgtgggcc gcgccgtcgg
     1981 gcgaggtgga agatttaagg tacatgaacg aggcggggcc gttggcgtgc gtgtgggact
     2041 tgcgcgtcgt cgggttcgag cgcgaggcgt gggtcgagga ggtgctggcg aacgccgcgg
     2101 ggccggacat cgaggcttac gtgaaccggc ggctgaacga agacgcttga gcgcgtgcgc
     2161 gcggcgcaga ggcggcgcgg gcgacaactc ctgaagaggt gcgcggcgtg ggcgaagggt
     2221 tcagggtcga gcgcgtgacg aaggacaact ggcaggaggc gatggcgctg gagatcgcgc
     2281 cggagcagcg ggcgttcgtg ccgtcggtca tgtactcgct ggccaaagcc ttcgtcgagc
     2341 cggacggcaa gaagtacgag ccttacgtcg tctacgcggg cgcagcggcg gtcggcttct
     2401 acatgctgtc gcacacgcct ggcgacgaga cgaagttcta tttcaccaac ttcctcatcg
     2461 gcagagagca tcaggggcgc ggccacggtc gcgcggcgct ggtcgattgt ctggagtcgg
     2521 tgaaggcgag gttcccggcg tgcgaggagg tctacctggc cgtccacccg gagaacgtag
     2581 tggcgacgcg gctctacgag agcctcggct tctacaagtt cgggttcgtc atcgacggcg
     2641 aggacgggat gcgcgtcaat cttaaaaaga tcggagagca aaaacagtga tcgtcggact
     2701 gccgaaggag atcaaggaca acgagtaccg cgtggggctg acgccggcgg gcgtgcgcgc
     2761 gctgacggac gcgggccatc aggtcaacgt cgagcgcgcg gcgggcgaag gctcgggctt
     2821 cgaggacgcg ctgtacgagc gcgcgggcgc gacgatcatc gagagcgcgg acgatgtgtg
     2881 ggccggcgcc gacatgatcg tcaaggtcaa ggaacccatc gcgcccgagt acccgcggat
     2941 gaaggaaggg ctgctgctct tcacctacct gcacctcgcg cccgaccgcg agcagacgaa
     3001 gcaactgctg gagcgtaaag tcacgggcat cgcctacgag acgatcaccg acaagcgcgg
     3061 gacgctgccg ctgctgacgc cgatgtcgga ggtggcgggg cggatggccg tgcaggtcgg
     3121 cgcgacgtac ctcgaacgga tgaacggcgg gcgcggcgtg ctgctgggcg gcgtgccggg
     3181 cgtgccggcg gcgcgcgtgg tcatcatcgg cggcggggtg gtggggacta acgccgccaa
     3241 gatcgccgtc gggatggggg cgcacgtcac gatcatcgac cgcgacctcg accgcctgcg
     3301 cgagctggac gacatctttc tctcgaagat ttcgacgctc gcctcgtcgg cctacgccat
     3361 ccacggcgcg atctccgagg ccgacctcat catcggcgcg gtgctcgtcc ccggcgcggc
     3421 ggcgccgcgg ctcgtcacgc gcgagatgct gaaggacgtg ccgaacggct cggtgatcgt
     3481 ggacgtggcg gtcgatcag
//
LOCUS       DBNGOH010000077         3829 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_11357, whole genome shotgun sequence.
ACCESSION   DBNGOH010000077 DBNGOH010000000
VERSION     DBNGOH010000077.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3829)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3829)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3829
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_11357"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2398
                     /locus_tag="AC159P_01485"
     CDS             <1..2398
                     /locus_tag="AC159P_01485"
                     /EC_number="2.2.1.2"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007080.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional transaldolase/phosoglucose
                     isomerase"
                     /protein_id="HIR3694151.1"
                     /translation="GININITMIFSIENYEQVAEAFIKGLERRAAEGKNVDHIASVAS
                     FFVSRVDTAIDSDLEYKARHAASPEEKAKFESLLGRAANANAKLAYRKFKEIFHGPRF
                     ADLLAKGAQVQRCLWASTGTKNPNYSDVLYVDNLIGPETVNTVPPATYTAIRDHGKVA
                     LTLEEGIEECHALFDQLREVGIDIKAVTEKLQKDGLASFVTSFDTLAESIESKRDAIL
                     SGIHERLTASLGKHSEAVAAAVKEADKGDTVRRIWRKDASLWKQEEEHQAIIKNALGW
                     LTVPDMMIGVEDDLIAFADRIRGARDFKHVMVCGMGGSSLCPEVLRQTFGKQDGYPEL
                     LVLDSTDPDAFSDIAEQIDITHCLFIISSKSGTTTEPLTFYKYWYDQVGKRKENPGEN
                     FVAVTDPGTKMEADATRDKFKRIFLNPADIGGRYSALSYFGMVPAALMGLDIEKLLDR
                     AERVVHACSQVVPAGENPGARLGAIMGECAKAGRDKLTIVADPKIASFGLWVEQLLAE
                     STGKDGKGIVPVAGEPLGGPSVYGDDRLFVSIAVGKPDSETEAKLKALEAAGHPVVYR
                     TLTDLYDLGEEFFLWEIATAFAGWRLGINPFDQPNVQESKDATKELLDTFKRDGKLPE
                     QPVLVKDDVLTIYADDKTREALPASSTAEAIKAHLARVGAGDYVALLNYIEETPEHEA
                     VIQQIRTHLRDATRCATTTGYGPRFLHSTGQLHKGGSDAGVFLQITAPDLKDLEIPGE
                     PYTFSTLKQAQALGDFRSLSTRGRRAIRVDLGADALAGLKRLHELVGEALPLADATAK
                     "
     gene            2506..>3829
                     /gene="gndA"
                     /locus_tag="AC159P_01490"
     CDS             2506..>3829
                     /gene="gndA"
                     /locus_tag="AC159P_01490"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011141117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HIR3694152.1"
                     /translation="MSAPEQTTQTAKIGMIGLAVMGENLARNIERNGYSVAVYNRPPG
                     RDGEPDRVTKFMEENAGKQFIGTRTPEEFIKALERPRKVILLVKAGDPVDWTVDQIKP
                     YLEAGDIIIDGGNSYFMDTERREKALKAEGFNFIGSGVSGGEKGALWGPSLMPGGDRE
                     AYEQIRPIWEAIAAKVDDGPCVTYIGPGGAGHFVKMVHNGIEYGDMQLIAEAYDIMRR
                     VLGMSADEMADVFDEWNRGDLESFLIEITAKILRVKDPETGKPLVDLVLDKAGQKGTG
                     KWTSAIALDLGVVTPTINASVDARNLSSRKEERVEAAKQIGGPQGGGYSGDRAEMIAA
                     VRDALYASKICSYAQGMNLIRAGSDNWKWDINLGEAARIWKGGCIIRAQFLGKISDAY
                     RRRPDLPNLLLDTDFNDYMQRAQDNWRRAVTTAIRLGIPVPGMAASVAY"
BASE COUNT          748 a         1325 c         1199 g          557 t
ORIGIN      
        1 cggcatcaac atcaacatca cgatgatctt ctccatcgag aactacgagc aggtggccga
       61 agccttcatc aaaggcctcg aacgccgcgc cgccgagggg aagaacgtcg atcacatcgc
      121 gtcggtcgcc agcttcttcg tctcgcgcgt ggacacggcg atcgactccg acctcgaata
      181 caaggcgcgt cacgccgctt cgcccgaaga gaaagcgaag ttcgaatcct tgctcgggcg
      241 cgcggccaat gccaacgcga agctcgccta ccggaagttc aaggaaatct tccacggccc
      301 gcgcttcgcc gacctcctgg cgaagggcgc gcaggtgcag cgctgcctgt gggcctcgac
      361 ggggacgaag aacccgaact attcggacgt gctctacgtc gataacctga tcggccccga
      421 gacggtcaac accgtgccgc ccgcgaccta cacggccatc cgtgaccacg gcaaggtggc
      481 gctcacgctc gaagagggga tcgaagagtg ccacgcgctc ttcgaccaac tccgcgaggt
      541 cggcatcgac atcaaggccg tcaccgagaa gttgcagaag gacgggctgg cctctttcgt
      601 cacctcgttc gacacgctcg ccgaatcgat cgagtcgaag cgcgacgcca tcctctccgg
      661 catccacgaa aggttgacag ccagcctggg aaagcactct gaggcggtgg ccgccgccgt
      721 caaggaggcc gacaagggcg acacggtgcg ccgcatctgg cgcaaggacg cctcgctgtg
      781 gaagcaggag gaggaacacc aggccatcat caagaacgcg ctcggctggc tcaccgtgcc
      841 ggacatgatg atcggcgtcg aggacgacct catcgccttc gccgaccgca tccgcggcgc
      901 gcgcgacttc aagcacgtca tggtctgcgg catgggcggc tcttccttgt gccctgaagt
      961 cttgcgtcag acattcggca agcaggacgg ctacccggag ctgctcgtcc tcgactcgac
     1021 cgacccggac gccttctccg acatcgccga gcagatcgac atcacgcact gtctcttcat
     1081 catctcctcg aagtcgggca cgaccaccga gccgctgacc ttctacaagt actggtacga
     1141 tcaggtcggc aagcgcaaag agaaccccgg cgagaacttc gtcgccgtca cagaccccgg
     1201 cacgaagatg gaggcggacg cgacgcgcga caagttcaag cgcatcttcc tcaacccggc
     1261 ggacatcggc gggcgctact cggcgctctc ctacttcggc atggtgcccg cggcgctgat
     1321 gggcctcgac atcgagaagc tgctcgaccg cgccgagcgc gtcgtccacg cctgttcgca
     1381 ggtcgtgccc gcgggcgaaa accccggcgc gcgcctcggc gcgatcatgg gcgagtgcgc
     1441 gaaggcgggc cgcgacaagc tgaccatcgt cgccgacccg aagatcgcct cgttcgggct
     1501 gtgggtcgag caactgctcg cggagagcac gggcaaggac ggcaagggca tcgtgccggt
     1561 cgcgggcgag ccgctgggcg gcccctcggt ctacggcgac gaccggctct tcgtctccat
     1621 cgccgtcggc aagcccgaca gcgagaccga ggcgaaattg aaggcgctcg aagccgccgg
     1681 ccaccccgtc gtctaccgca cgctgacaga cctctacgac ctcggcgaag agttcttcct
     1741 ctgggagatc gccacggcgt tcgccggctg gcgcctcggc atcaacccct tcgaccagcc
     1801 gaacgtgcag gagtcgaagg acgcgacgaa ggagctgctc gacaccttca agcgcgacgg
     1861 gaagttgccc gagcagcccg tgctcgtcaa ggacgacgtg ctcaccatct acgccgacga
     1921 caagacgcgc gaagcgctgc ccgcgtcctc gaccgccgaa gccatcaagg cgcacctggc
     1981 gcgcgtcggc gcgggcgact acgtcgcgct gctgaactac atcgaggaga cgcccgagca
     2041 cgaggcggtg atccagcaga tccgcacgca cctgcgcgac gcgacccgct gcgcgaccac
     2101 gaccggctac ggcccgcgct tcctgcactc aaccggccag ctccacaagg gcggctccga
     2161 cgcgggcgtc ttcctgcaaa tcaccgcgcc cgaccttaag gatttggaga taccgggcga
     2221 gccgtacacc ttcagcacct tgaagcaggc gcaggcgctc ggcgacttcc gctcgctctc
     2281 cacgcggggc cgccgcgcca tccgcgtcga tctcggcgcc gacgcgctcg ccggcctcaa
     2341 gcggctccac gaactcgtcg gcgaagcgct cccactcgcg gacgcgacgg caaaataaaa
     2401 gcgggtgttg ggtgttaggt gctcggtttt gggttttttt cttccccaac acctaatacc
     2461 tgacacccaa aaccctgatt cattcccctg ttgaggacaa cgacaatgtc agcacccgaa
     2521 cagaccacgc agaccgccaa gatcggcatg atcggcctcg ccgtgatggg cgagaatctg
     2581 gcgcgcaaca tcgagcgcaa cggctactcg gtcgccgtct acaaccgccc gccgggcaga
     2641 gacggcgaac ccgaccgcgt gaccaagttc atggaagaga acgcgggcaa gcagttcatc
     2701 ggcacgcgca cgcccgagga gttcatcaag gcgctggaaa ggccgcgcaa ggtcatcctc
     2761 ctcgtcaagg cgggcgaccc cgtcgattgg acggtcgatc agatcaagcc gtacctcgaa
     2821 gcgggcgaca tcatcatcga cggcggcaac tcctacttca tggacaccga gcggcgcgag
     2881 aaggctttga aggccgaggg cttcaacttc atcggctcgg gcgtctcggg cggcgagaag
     2941 ggcgcgctgt gggggccgtc tctgatgccg ggcggcgacc gcgaagccta cgagcagatt
     3001 cgccccatct gggaggccat cgcggcgaag gttgacgacg ggccgtgcgt cacctacatc
     3061 gggccgggcg gcgccgggca cttcgtcaag atggtgcaca acggcatcga gtacggcgac
     3121 atgcagctca tcgccgaggc ctacgacatc atgcgccgcg tgctcggcat gtcggcggac
     3181 gagatggccg acgtgttcga cgagtggaac cgcggcgacc tcgaatcgtt cctcatcgaa
     3241 atcaccgcca agattctgag agtcaaagac cccgagacgg gcaagcccct cgtcgatctc
     3301 gtgctcgata aggcgggaca gaagggaacg gggaagtgga cgagcgccat cgcgctcgac
     3361 ctcggcgtcg tcacgccgac catcaacgcc tcggtggacg cgcgcaacct ctcttcgcgc
     3421 aaagaagagc gcgtcgaggc cgccaagcag atcggcgggc cgcagggcgg cggctacagc
     3481 ggcgaccgcg ccgagatgat cgccgccgtg cgcgacgcgc tctacgcgtc gaagatctgc
     3541 tcctacgcgc agggcatgaa cctgattcgc gccggctccg acaactggaa gtgggacatc
     3601 aacctcggcg aggcggcgcg catctggaag ggcggctgca tcatccgcgc gcagttcctc
     3661 ggcaagatca gcgacgccta ccgccgccgc cccgacctcc cgaacctcct cctcgacacg
     3721 gacttcaacg actacatgca gcgggcgcag gacaactggc ggcgggccgt gacgacggcc
     3781 atccgcctcg gcatccccgt ccccggcatg gcggcctcgg tcgcctact
//
LOCUS       DBNGOH010000078         2433 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_928364, whole genome shotgun sequence.
ACCESSION   DBNGOH010000078 DBNGOH010000000
VERSION     DBNGOH010000078.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2433)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2433)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2433
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_928364"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..292)
                     /locus_tag="AC159P_01495"
     CDS             complement(<1..292)
                     /locus_tag="AC159P_01495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694153.1"
                     /translation="MYCPKCSQQQPTDDVRFCTRCGLPLSGVAELMATGGALVRRPAG
                     AGDQRLSPRQKGVRMGALLMLTCSLIALVQALMPSPVDKLVVLAAPVIIGFFV"
     gene            complement(316..>2433)
                     /locus_tag="AC159P_01500"
     CDS             complement(316..>2433)
                     /locus_tag="AC159P_01500"
                     /EC_number="3.1.4.59"
                     /inference="COORDINATES: protein motif:HMM:NF019318.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0106409 - cyclic-di-AMP phosphodiesterase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD family phosphohydrolase"
                     /protein_id="HIR3694154.1"
                     /translation="AQSFRASWSALKQQADARDGDSTDNDGANVNSAAQAARAELAWP
                     GEGDDKQAVARAVAARNFDAGALDFITRAIREAGSLYVFDDREAEHVSGDMRVVDTRG
                     GTQTAVNQFQERFVSLAAARESLRERILKLTNWKVAEREAIATAALPLLKTNVTYDRA
                     ATDAAREAAAQTVQSTLVVLKRNQTIARAGDTATPQMLAQFAALRDYSHTERRPQHFV
                     GLFLLACALYFAAWRFTEYRSTITALPLSHARAFTLVCLSVLVQLVLMRAGFFVAESI
                     ASRSGSAPMNDVTIWSFAIPFAAAALLVATLVDTQLALITAVLSAIFAGLLAPGGTLI
                     TCFSLASSSAAIYGFGRYSNRQSVMQAGLVAGLVNVVLALAVMLAAQQPLTLNAILLA
                     AACGLGGGFLTAVFAAGGLPVNESLFGILTDVKLLELSNADLPVLGQLALRAPGTNQH
                     SHAVGHLAEEGCRAIGANPLLARIGALYHDIGKLAAPEYFVENQSGDNPHDRLKPTQS
                     ARIITSHVSYGQKLAGEINLPRQIADFIPQHHGTRTLHFFLRKAQAHAAEGETVDESE
                     FRYPGPKPQSKEAAVMMLADSCEAAARSLARPDPENIRSIVVKIFEAIISDGQLDECN
                     LTMRELTKLRESVIASLTAIYHARIDYPGFNPPSLDPNQSPLPRPEIDSEERGIGYTR
                     PSEVPISKGGEVEDEAVTSKLNP"
BASE COUNT          360 a          677 c          999 g          397 t
ORIGIN      
        1 cgacgaagaa gccgatgatg acgggcgcgg cgaggacgac gagcttgtct acgggcgaag
       61 gcatcagcgc ctgaacgagc gcgatgagcg agcaggtgag catgagcagc gcgcccatcc
      121 tcacgccttt ctgccggggc gagaggcgtt ggtcgcccgc gccggcggga cgacgaacga
      181 gcgcgccgcc ggtggccatc aactccgcga cgccgctcaa ggggaggccg cagcgggtgc
      241 agaaacgcac gtcgtcggtc ggttgctgct ggctgcactt tggacagtac atgacgactg
      301 ctcccttgtt cggcgttaag ggttcagctt gctcgtcacg gcttcgtctt cgacctcgcc
      361 gcctttgctg atggggactt cggaggggcg cgtgtagccg atgccgcgct cttcgctgtc
      421 gatttcgggg cggggcagcg gcgactggtt ggggtcgagc gagggcgggt tgaagccggg
      481 gtagtcgatg cgcgcgtggt agatggccgt gagcgaggcg atgacggact cgcgcagctt
      541 ggtcagctcg cgcatggtga ggttgcattc gtcgagctgg ccgtcgctga tgatggcctc
      601 gaagattttg acgacgatgg agcggatgtt ttcggggtcg ggccgggcga gcgagcgcgc
      661 ggcggcttcg cacgagtcgg cgagcatcat cacggcggcc tctttggact gcggcttggg
      721 gccggggtag cggaactccg actcgtcaac ggtctcgcct tcggcggcgt gggcctgcgc
      781 cttgcggagg aagaagtgga gcgtgcgcgt gccgtggtgc tgcgggatga agtcggcgat
      841 ctggcgcggg aggttgatct cgccggcgag cttctgcccg tagctgacgt ggctggtgat
      901 gatgcgcgcg ctctgcgtcg gcttgaggcg gtcgtgcggg ttgtcgccgg actgattctc
      961 gacgaagtac tcgggcgcgg cgagcttgcc gatgtcgtgg tagagcgcgc cgatgcgcgc
     1021 gagcaagggg ttcgcgccga tggcgcggca accttcttcg gcgaggtggc cgacggcgtg
     1081 cgaatgctgg ttggtgccgg gcgcacgcag cgccaactgt ccgaggacgg gcagatccgc
     1141 gttcgagagt tccaagagct tcacgtcggt gaggatgccg aagagcgatt cgttgacggg
     1201 cagcccgccg gcggcgaaga cggcggtcag gaatcccccg ccgagtccgc acgcggcggc
     1261 gagcaggatg gcgttcaagg tcaggggctg ctgcgccgcg agcatgacgg cgagcgcgag
     1321 gacgacgttg acgagtcccg cgacgagtcc cgcctgcatg accgactggc ggttggagta
     1381 gcgaccgaag ccgtagatgg cggcggagga ggacgcgagc gagaagcagg tgatgagcgt
     1441 gccgccgggc gccaagagtc cggcgaagat ggccgagagg acggcggtga tgagcgcgag
     1501 ctgcgtatcg acgagcgtgg cgacgaggag cgcggcggcg gcgaagggga tggcgaagct
     1561 ccagatggtc acgtcgttca tgggcgcgct cccggagcgc gaggcgatgg actcggcgac
     1621 gaagaagccc gcgcgcatga ggacgagctg gacgaggacg gagaggcaga cgagcgtgaa
     1681 ggcgcgcgcg tgcgacaagg ggagcgcggt gatcgtgctg cggtattcgg tgaagcgcca
     1741 ggcggcgaag tagagcgcgc aggcgaggag gaagaggccg acgaagtgtt gggggcggcg
     1801 ctcggtgtgg ctgtagtcgc gcagggccgc gaactgcgcg agcatctgcg gcgtggcggt
     1861 gtcgccggcg cgcgcgatgg tctggttgcg tttgaggacg acgagggtgg actggacggt
     1921 ctgcgcggcg gcctcgcgcg ccgcgtcggt cgcggcgcgg tcgtaggtga cgttggtctt
     1981 gagcaggggc agggcggctg tggcgatggc ctcgcgctcg gccaccttcc agttggtgag
     2041 cttcaggatg cgttcgcgca gggattcgcg cgcggcggcg agcgagacga agcgctcctg
     2101 gaactggttg acggcagtct gcgtgccgcc gcgcgtatcg acgacgcgca tgtcgccgct
     2161 gacgtgttcg gcctctctgt cgtcgaagac gtagaggctg cccgcttcgc ggatggcgcg
     2221 cgtgatgaag tcgagcgcgc ccgcgtcgaa gttgcgcgcg gcgacggcgc gggcgacggc
     2281 ctgtttgtcg tcgccttcgc cgggccacgc gagttcggcg cgggcggcct gcgcggcgga
     2341 gttaacgttc gcgccgtcgt tgtcggtgct gtcgccgtcg cgcgcgtccg cctgttgttt
     2401 gagcgcagac caggaggcgc ggaaagattg tgc
//
LOCUS       DBNGOH010000079         6287 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4594277, whole genome shotgun sequence.
ACCESSION   DBNGOH010000079 DBNGOH010000000
VERSION     DBNGOH010000079.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6287)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6287)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6287
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4594277"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..312)
                     /locus_tag="AC159P_01505"
     CDS             complement(<1..312)
                     /locus_tag="AC159P_01505"
                     /inference="COORDINATES: protein motif:HMM:NF014465.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mercuric transporter MerT family protein"
                     /protein_id="HIR3694155.1"
                     /translation="MKERLATGGAVAAAAAASLCCIGPLVFVALGLGAFGASAVFETL
                     RPYLPSAAVLLFAFGFYRAYFRRSGAACAPGEACTTNPSGRATRVALWVAAFSVIAFA
                     LS"
     gene            complement(309..608)
                     /locus_tag="AC159P_01510"
     CDS             complement(309..608)
                     /locus_tag="AC159P_01510"
                     /inference="COORDINATES: protein motif:HMM:NF020839.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding protein"
                     /protein_id="HIR3694156.1"
                     /translation="MRFIKQAQEIGLSLDEIGGLLAEGGAAECQRVHDLLRAKLEELD
                     GRIKAMRAFRRTLARHYAACEKELGERGEEARCPVVIEITHTAHEEAGAGKVKRR"
     gene            complement(636..761)
                     /locus_tag="AC159P_01515"
     CDS             complement(636..761)
                     /locus_tag="AC159P_01515"
                     /inference="COORDINATES: protein motif:HMM:NF012594.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIR3694157.1"
                     /translation="MSQSELRIGELASRAGVSVDTVRFNERRRRLPRAHRTGGRF"
     gene            complement(854..1459)
                     /locus_tag="AC159P_01520"
     CDS             complement(854..1459)
                     /locus_tag="AC159P_01520"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR3694158.1"
                     /translation="MSEARMRVEPHAPPYTIETCLTDLESVRAHYGVNRWVIGGHSWG
                     ADLALIYAMEHAPHVAGLICIAGGRVHNDREWHAEYERRKKQEREHVPRFDYPPNMEV
                     NRQLNRSWKRYIQTPELLSAISRLAVPALFVYGERDIRPSWPVEQVARLMPDARFELI
                     EGAEHVIWFSHPNELKPLLRTFVGEVGRRSQPNSQSPTPRH"
     gene            1466..1612
                     /locus_tag="AC159P_01525"
     CDS             1466..1612
                     /locus_tag="AC159P_01525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694159.1"
                     /translation="MRETEAWREMTRLNERTLESLDALFDPDDPAEEVFDFPEDEYEP
                     FEFR"
     gene            1698..3827
                     /locus_tag="AC159P_01530"
     CDS             1698..3827
                     /locus_tag="AC159P_01530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694160.1"
                     /translation="MRSISGARRTARRRAPRRSSRFALLRRRLPALVVCLPLLAFSLD
                     AALAQTSAPTPPPVAPPPAGPPRQVRDLSAVAERFVPAVKRTVEGAMLKRYTFLATVF
                     AQLVMLAALIRLQADKPGATRDFFGQCGRAVVILPLVVLGPWLVSYLYTLGGQLVFPL
                     RVPLRAAYAEFDESYTRFTLGIFTATDRGGVYQPMPTGTASVVGVLADRESTVKTVDE
                     MVDPARWDMTKLFTFMNIARGILSFGMFVLVVLTGFLMIAFRLAVPWMIAMSIDRSLA
                     HEVSYKFARGVVVYTLVFPAVAHILMLIPFKIGTLGMAVYDGTPMYDVDPQTARLIAR
                     PDVDPTFCFGVAVFMMGVAALCFVAAPVLSWKIAFGQTFEGVAMVASGWMAAIVGSGI
                     NLVSARLGASLNNMAERLPVETSANAGLTTARADYNATTRINAAGLHNQLGQIAAGRT
                     GAIMTNNAAAAREQTNLLAAYRNSMANVAISRDAQVSVIEADRSYGTRGAQNATAREQ
                     GQVLLNQQVGENSNEQAWWSFGTEVVGGAGGVAAGGAGGATPGQQAGRLASRPGEIVT
                     DSVNIEMQARGGLGLSSQYLSRTAENNRTYAEDRTEVEQTRATQTEGALNEQYGAQSG
                     AISTWQQSVNDAANAQAAISSTAARESTGMLDRAALTKYAGAESAINQVRSAGLQAAE
                     YHRMAQIIGQVTHDMTRRIEEMGQYRF"
     gene            4100..5227
                     /locus_tag="AC159P_01535"
     CDS             4100..5227
                     /locus_tag="AC159P_01535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694161.1"
                     /translation="MTRDGRLAHCMKESAGTVKRAKDGGYVHVLVEDRIPFGATRPDT
                     ISTTNGLPRRPAPGPTAVVPLEVRYAAYQKLIALSPASLYPRELVNATPDGLLARGLT
                     PQDVHRFGALPPRVRERDELARVIDRFIEEQFPAYVEAHRMRGVIGIPGFWLKGHTLV
                     KLGKDYDYERPALVIPYHDEQGSMQACQLRFPGRRGGYHWLSTAEDCLDREAHGTSSG
                     CPIHWTAPPGETLGCKGLPILVTEGALKAEVFVSLRPPMRAVATAGVGVAHAEIVRAL
                     RGRDAIIGFDGDHRENAQVCRQLGKLIAEREQDALRIGQKNSTSVVIWEGAKGIDDAV
                     RRNVRLRVLGVWEWRATLKGKPFKGVEDVWAALTFAPAGGC"
     gene            complement(5509..5835)
                     /locus_tag="AC159P_01540"
     CDS             complement(5509..5835)
                     /locus_tag="AC159P_01540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694162.1"
                     /translation="MKLTSKDLSLDEAVLALLRAADRGVRVFTPDGETPEALADFQQV
                     VRLLRMMEGRRYVAAICSLNLLAASGGQSRVDRVRLSGGLTDKGRAVLAYHDGEARGH
                     LNSQTA"
     gene            complement(5853..>6287)
                     /locus_tag="AC159P_01545"
     CDS             complement(5853..>6287)
                     /locus_tag="AC159P_01545"
                     /inference="COORDINATES: protein motif:HMM:NF013828.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR3694163.1"
                     /translation="KTHLAAAVLHGLVGKGVSCLFYEFGALLKEIQHSYNPVSRASEP
                     KVLAPVYEAEVLVLDELGASKPTDWVRDTMMQIIGARYNERRLTIFTTNYPDARRQPA
                     DETLEDRVGARLRSRLYEMWKTVIIGGEAYRRRLAAQAWGET"
BASE COUNT         1038 a         2152 c         2115 g          982 t
ORIGIN      
        1 cgagagcgcg aaggcgatga cggagaaggc ggccacccag agcgccaccc gcgtcgcccg
       61 gccggaggga ttcgtcgtgc aagcctcgcc cggggcgcac gccgctcccg agcgccggaa
      121 gtaggcccgg tagaacccga aggcgaacag cagcacggcc gcgcttggaa gatacgggcg
      181 cagggtctcg aagacagcgg acgccccgaa cgcacccagg cccagcgcga cgaagacgag
      241 cgggccgatg cagcacaggc tcgccgccgc cgcagcggcc acagcccctc ccgttgcgag
      301 tctctctttc atctcctctt caccttgcct gcgcccgctt cctcgtgtgc ggtgtgggtg
      361 atctcgatga ccaccgggca ccgcgcctcc tcgccgcgct cgccgagctc tttctcgcag
      421 gccgcgtagt ggcgggccag cgtgcggcgg aaggcgcgca tggccttgat cctcccgtcc
      481 agctcttcga gcttcgcgcg caggaggtcg tgaacgcgct ggcactctgc cgcgccgcct
      541 tccgcgagta gcccaccgat ctcatcgagc gagaggccga tctcctgcgc ctgcttgatg
      601 aagcggatgc gctcgaacgc ctccgccggg aaaagtcaaa agcggccccc ggtgcggtgc
      661 gcgcggggca gccgccgccg gcgctcgttg aagcgcacgg tgtcgacgga gacgcccgcg
      721 cgcgaggcca actccccgat gcgaagctca gactgcgaca cggcggtcta atcctccttc
      781 ggcaccgatt agattctaca atctggagct aagtccagag tcaagatgaa tctgaggtgc
      841 ggtggcgggg gggttagtgg cgcggggtag gactttgcga gttgggttgc gaccgccggc
      901 cgacttcgcc gacgaacgtc cggaggagcg gcttgagttc gttcggatgt gagaaccaga
      961 tgacgtgctc ggccccctca atcaactcga agcgcgcgtc gggcatcagg cgggccacct
     1021 gctcgaccgg ccaactcggg cggatgtccc tctcgccgta gacgaagagc gccggcaccg
     1081 ccagccgcga aatcgcactc agcaactcgg gggtctggat gtagcgcttc caggagcggt
     1141 tgagctggcg gttcacctcc atgttgggcg ggtagtcgaa gcggggcacg tgctcccgtt
     1201 cctgcttctt cctgcgttcg tattcggcgt gccactcgcg gtcgttgtgt actcgcccgc
     1261 ccgcgatgca gatcagaccc gccacgtgcg gcgcgtgctc catcgcgtag atgagcgcca
     1321 ggtcagcgcc ccacgagtgc ccgccgatga cccagcggtt aactccgtag tgtgcgcgga
     1381 cgctctccag atccgtcagg caagtctcga ttgtataggg cggggcgtgc ggctcgacgc
     1441 gcatgcgcgc ttcggacagg cggtcgtgcg cgagaccgag gcgtggcgcg agatgacccg
     1501 cctcaacgag cggacgctgg agagcctcga cgcgctcttc gacccggacg acccggcgga
     1561 ggaggtcttc gacttccccg aagacgagta cgagccgttc gagttccgct gaagtcgcgg
     1621 gcgacagaga gtcgccggaa gatggcgtgg gcgggtgcgc cccgccgggg tgaaggacga
     1681 ttccgatgtt caggttcatg cgctcaatat ccggcgcgag acggactgcc cggcggcgcg
     1741 ccccgcggcg ctcgagccgc ttcgcgctgc tccggcggcg gctgccggcg ctcgtcgtct
     1801 gcctcccgct cctcgccttc tccctcgacg ccgccctggc ccagacctcg gcgccgacgc
     1861 ccccgcccgt ggccccgccc cccgccggac cgccgcggca ggtgcgcgac ctgtcggccg
     1921 tcgcggagcg gttcgtcccg gccgtcaagc gtacggtcga gggcgcgatg ctcaagcgct
     1981 acaccttcct cgccaccgtc ttcgcgcagc tcgtcatgct cgccgccctc atcaggctgc
     2041 aggcggacaa gcccggcgcg acgcgcgact tcttcgggca gtgcgggcgc gccgtcgtca
     2101 tcctgccgct cgtcgtcctc gggccgtggc tcgtcagtta cctctacacc ctcggcggcc
     2161 agctcgtctt cccgctccgc gtcccgctgc gcgcggccta cgcggagttc gacgagtcgt
     2221 acacgcgctt cacgctgggc atcttcacgg cgaccgaccg cggcggcgtc taccagccga
     2281 tgccgacggg gacggcctcg gtcgtcggcg tgctcgccga ccgcgagagc acggtgaaga
     2341 ccgtggacga gatggtcgac ccggcgcggt gggacatgac gaagctcttc accttcatga
     2401 acatcgcgcg cgggattctc tccttcggca tgttcgtgct cgtcgtgctg acgggcttcc
     2461 tgatgatcgc cttccggctg gctgtcccct ggatgatcgc gatgagcatc gaccgctcgc
     2521 tggcgcacga ggtctcgtac aagttcgcgc gcggggtggt ggtctacacg ctcgtcttcc
     2581 ccgccgtcgc gcacattttg atgctcatcc cattcaagat cggcacgctg gggatggcgg
     2641 tctacgacgg cacgccgatg tacgacgtcg acccgcagac cgcccggctc atcgcccgcc
     2701 cggacgtcga cccgacgttc tgcttcgggg tggccgtctt catgatgggc gtcgcggcgc
     2761 tctgcttcgt cgccgcgccg gtgctctcgt ggaagatcgc cttcggtcag acgttcgagg
     2821 gagtcgcgat ggtggcttcg gggtggatgg cggcgatcgt cggctccggc atcaacttgg
     2881 tgagcgcgcg cctcggcgcg tcattaaaca acatggccga aagactgccg gttgagactt
     2941 cagccaacgc cgggctcacc accgcccgcg ccgattacaa cgcgacgact cggatcaacg
     3001 ccgccggcct tcataatcaa ctgggccaga tagcggcggg acgtaccggc gcgatcatga
     3061 ccaacaatgc ggcggccgcg cgcgagcaga ccaacctgct cgccgcgtac cgcaacagca
     3121 tggcgaacgt cgcaatcagt cgcgacgcgc aggtgagcgt catcgaagcc gaccggtcct
     3181 acgggacgcg cggcgcgcag aacgcgacgg cgcgagagca gggacaagtt ttacttaacc
     3241 aacaggtcgg cgagaactcg aacgagcagg cgtggtggtc gttcgggacg gaagtggtag
     3301 gcggggccgg cggggtcgcg gcgggcggcg cgggcggcgc gacgccgggg cagcaagcgg
     3361 gaaggctcgc cagccgtccg ggcgagatcg tcaccgacag cgtcaacatc gaaatgcagg
     3421 cgcggggcgg tctggggctt tcgagccagt acctgagccg caccgccgag aacaaccgca
     3481 cctacgccga ggaccgcacc gaggtcgagc agacgcgggc cacgcagacc gagggcgcgt
     3541 tgaacgagca gtacggcgcc caatccggtg cgatctcgac atggcagcag agcgtcaacg
     3601 acgcggcgaa cgcgcaagcc gcaatttcct cgaccgccgc gcgcgaaagc accgggatgc
     3661 tcgaccgcgc tgccctgacg aagtacgccg gagccgagag cgccatcaat caggtgcgct
     3721 ccgccggtct gcaagccgcc gagtatcacc gaatggcgca gatcatcggc caggtcacgc
     3781 acgacatgac gcgccgcatc gaggagatgg ggcagtaccg cttctagcgg aagggcggca
     3841 agcgaccgcc ggttcgaaaa tcgagcgggc cgcggatcgt gcgatgacgg gcggacgaca
     3901 ggaacgtcag gcgcccgtcg cggcaccact tctccgaaag gttccggggc cgcccgtgag
     3961 ttacgacgcg atgacgcgcg aaaccggcag gtcttttacc gtatcgccgg ggcgctaccc
     4021 cgaacgctcg cgcgtcggct tcacgcgggc ggcgcggagg aaaccgtgcc cgatctgcgg
     4081 cgcgaagaag tactgtcagg tgacgcgcga cggcaggctc gcgcactgca tgaaagagtc
     4141 cgcggggacg gtcaaacgcg cgaaagacgg cggctacgtg cacgtgctcg tcgaagaccg
     4201 gatcccattc ggcgccaccc gcccggatac gatttcgact acgaacggac tgccccgccg
     4261 cccggccccc ggtccgacag ccgtcgtccc gctcgaggtc agatacgccg cgtatcagaa
     4321 gctgatcgcg ctctcgcccg cgtccctcta cccgcgcgag ctcgtcaacg ccacgcccga
     4381 cggtctgctc gcgcggggac tcacaccgca ggacgttcac cggttcgggg cgctgccgcc
     4441 gcgcgtccgc gaacgcgacg agttggctcg cgtgatcgat cgcttcatcg aggaacagtt
     4501 cccggcctac gtggaggcac accggatgcg cggcgtcatc ggcatacctg gcttctggct
     4561 gaaaggtcac accctggtca aactcggcaa agactatgac tacgaacgcc ccgccctcgt
     4621 catcccctac cacgacgagc aaggctcgat gcaagcctgc caactccgct tccccgggcg
     4681 gcgcggcggc taccactggc tctcgaccgc cgaagactgc ctcgaccgcg aggcgcacgg
     4741 cacgagctct ggctgtccca tccactggac ggcgccgccc ggcgagacgc tcggctgtaa
     4801 aggcctgccc atcctcgtca ccgaaggcgc attgaaggcc gaagtcttcg tctccctgcg
     4861 tccgccgatg cgcgccgttg cgaccgcggg cgtcggcgtc gcacacgccg agatcgtccg
     4921 ggcgctcagg gggcgcgacg cgataatcgg cttcgacggc gaccaccggg agaacgcgca
     4981 ggtctgccgc cagctcggga aactgatcgc cgagcgggag caggatgcgc tgcggattgg
     5041 gcagaagaac agtacgagtg tcgtgatttg ggaaggcgcg aaagggattg atgatgcggt
     5101 gcgccggaat gtgcgcctcc gcgtcctcgg cgtctgggag tggcgtgcga ccctgaaggg
     5161 aaaaccgttc aaaggcgtcg aagacgtgtg ggcggcactc acgttcgccc ccgcgggggg
     5221 ttgttagatg gactacccgc acgtcagatc gagtacggcc tgccccttgt gccgggggcg
     5281 caaagaggtc ggcctggtgg tctgctggtc atgttaccgc acgtggggta tacgaaacgg
     5341 gaacgcggaa gcggagtcga tcatcgaacg ggccgaagcc aaagccgaat tccgcgaggc
     5401 ccgcggggcg gcccgcccct agtcctcgaa cagagccggg gcgtcccgag ccggcatggt
     5461 tctatgttta tcgggcggtg aggaaaagtg ttgccatgcg ctccccggtc aggcggtttg
     5521 ggagttcagg tgcccccgcg cctccccgtc atggtaggcg agcaccgccc gccctttatc
     5581 cgtcagcccg ccgctcagcc gcaccctgtc aacccggctc tgaccgccac tggcggccag
     5641 taggttgaga ctacagatcg ccgcgacgta gcggcggccc tccatcatcc tgagcagcct
     5701 gacgacctgt tggaagtcgg cgagcgcctc gggcgtctcg ccgtccggcg tgaagacccg
     5761 cacgccccgg tcggcggcgc gcagcagggc gagcacggcc tcgtcgagcg acaaatcttt
     5821 cgacgtcagt ttcaccgttc gagtcttcgt ggtcatgttt ccccccaagc ttgcgcggcg
     5881 agcctgcgcc ggtaagcctc gcccccgatg ataacagttt tccacatctc gtagaggcgg
     5941 ctgcggaggc gcgcccctac gcggtcctcc agcgtctcat ccgcgggctg ccggcgcgcg
     6001 tccgggtagt tggtggtaaa gatcgtcagc cgtcgctcat tatagcgcgc gccgatgatc
     6061 tgcatcatcg tatcacgaac ccagtcggtc ggcttcgagg cgccgagttc atcaagcacc
     6121 agcacttcgg cctcgtagac gggcgcgagc accttcggct ccgacgcccg cgagacgggg
     6181 ttgtaggagt gctgaatctc cttgaggagc gcgccgaatt cgtaaaacaa gcaagatacg
     6241 cccttcccca caagcccgtg taagaccgcc gccgcgaggt gcgtctt
//
LOCUS       DBNGOH010000080         5217 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_561778, whole genome shotgun sequence.
ACCESSION   DBNGOH010000080 DBNGOH010000000
VERSION     DBNGOH010000080.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5217)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5217)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5217
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_561778"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3340
                     /locus_tag="AC159P_01550"
     CDS             <1..3340
                     /locus_tag="AC159P_01550"
                     /inference="COORDINATES: protein motif:HMM:NF051698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor domain-containing
                     protein"
                     /protein_id="HIR3694164.1"
                     /translation="VVRGSDTVRADAQLEVGGTSESVVVQADAGVIQTETPTIGGSIS
                     NQQLVELPRESRDIYQFLYLNPNITQGPADGDGFKFIGSQSYGASFSLDGQRANGGIF
                     GGVTASQPSLEVIQELTVLSNSFSAEYGGVANIRVVTKRGGSDYHGSLFYNNRNSALA
                     AWRVQDKNAKATFTPTPALSSFPTPYFNLNEVGGSFSGPVPKSSKTFFLTSYERRWDF
                     APVTLRASDMPTSTILGGDFRGLAETTRPAVPAEILPLLTPEELANNTFLTGTTRRFN
                     VIPERLLNPIALSLVNTYYPQTGNAPFNPVNGRLIDFYQNIPGLITRDLFTGRIDHDF
                     SDKDKFYAVYNYQKRNGVRALTANLPAFGLREQEGANHTLALSYTRVFSPTVVNEARG
                     GFNTQNLYTHARQTTQEFLAGLGFNDQEIASYGSIVGPSLLDTFGHLAFTLGSFRGIA
                     SGARNADRSFDQSLITFGDTVTIITGQHTIKGGADFIRNQAIDDFVKNRNDPRGTIIY
                     PNSFAGFARFLIGLPPSSVRYVFNVRPSMDVHNWETGFFIQDDWKLHSRLTLNLGLRY
                     DLITPFVDKNDLMINFDPNATGNNGNKGRFIVPSADIIPTLLPAFVSYGVVTAEEAGV
                     GRGLIEVDRNNLAPRLGLAWRVTDNTVIRGGFGMFYPTSAAQGQRDAFASTAFNQRIT
                     KNSTTAAPLAGLPGGINPRGLTPFSGGTFAVQGLSVNAIPTDLQSPRIDQFNVTVERD
                     LGWKTGLRVSYLGSRMHGLIAGTDLNLLPPSDIPFGTTTGDGVTPCVPNDFNCQISAA
                     DRARLPFPLLGDFLASYGNIGSGKSDALQIEANRRFDNGLTFNVSYTLLDQKSSGLDV
                     GASSLGGTLYNQFNPEGDYSRDGFVSRHRVVYYGAYDLPFGRGRRFGRESSRVLDAVA
                     GGWQLTWNGFAKSGTGFTPFWTCTNCSPAFPGNIASSFIDPVGGFGGVVGFRATVVSS
                     AEPYLRQGDQFFNPAAFGIPSAGADVLDNPAGAKRNFLTGPGTWGANLGLRKYFKFSE
                     TTRLEVGADFNNIFNHPLKSPQDPYFARVGEFAIAVDPATKKVIIPASGVTRNPLFGR
                     TIDSFRQEGIEDRRLIRVKLRLTF"
     gene            3479..4717
                     /locus_tag="AC159P_01555"
     CDS             3479..4717
                     /locus_tag="AC159P_01555"
                     /inference="COORDINATES: protein motif:HMM:NF012692.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HIR3694165.1"
                     /translation="MSSEDRTLQVSTRKPNLSNVKVAGHDVLRTVNRRILLNILSERQ
                     PISRADIAKLSGLNKATVSAITSALLKDHFIVEEGSGRTTPIGGKPPTPLRLNELRFG
                     ILGLDVRADETILALSDFHNRLVARKSYETGNDPRAFLTEIGGEITRLRAEHTQFIEV
                     AGIGVSLPGLVDNRAGKFISSVVLPWRDVPVVEWLRESTRLPVIIDNSARCAALAEIW
                     HSKSHYAHVRNLLYVSVSSGLACGVIIDGSLYRGWTNAAGQFGHIPIDFNGPECRCGQ
                     RGCWDLYASDNATVARYRNLPGATARRGVTMRKIVELVESGDPAATETVRETARYLGI
                     GIAGLVNGLDPEVVAIGGEIAKAWGVVEPIIAREMGRNMLASAGRKIELRRSTFDVRP
                     SLKGALTLVLSELLSVPHLG"
     gene            4733..>5217
                     /locus_tag="AC159P_01560"
     CDS             4733..>5217
                     /locus_tag="AC159P_01560"
                     /inference="COORDINATES: protein motif:HMM:NF012904.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HIR3694166.1"
                     /translation="MTQTAERIRARLDGGLIPAVPVTLDERGRIHARAHDAYLRHMSD
                     QPHAGVAVWAHTGRGLMLDREMADAVLKDWRAALPGGVVVAGAGAPPTADPSEATDRA
                     VEMAERAARLGADALLAYAPAWLRGREDRDRRIVEHHRRLARVGLPLVLFYLYEEAGG
                     VG"
BASE COUNT          895 a         1938 c         1577 g          807 t
ORIGIN      
        1 ggtggtgcgc ggcagcgaca cggtgcgcgc cgacgcccag ctcgaagtcg gcggaacctc
       61 ggagtcggtc gtggtgcagg cggacgcggg cgtcatccag acggagacgc cgaccatcgg
      121 gggctccatc agcaaccagc agctcgtcga gctgccccgc gagagccgcg acatctacca
      181 gttcctctat ctcaacccga acatcacgca gggcccggcc gacggcgacg gcttcaagtt
      241 catcggctcc cagagctacg gcgccagctt ctcgctcgac gggcagcgcg ccaacggcgg
      301 catcttcggc ggcgtcactg ccagccagcc gtcgctcgaa gtcatccagg agctgacggt
      361 gctctcgaac agcttctcgg ccgagtacgg cggcgtcgcc aacatccgcg tcgtcaccaa
      421 gcgcggcggg agcgactacc acggctcgct cttctacaac aaccggaact cggcgctggc
      481 ggcctggcgc gtccaggaca agaacgcgaa ggcgaccttc acgccgaccc ccgcgctctc
      541 cagcttcccg acgccctact tcaaccttaa cgaggtcggg ggctccttca gcggccccgt
      601 gcccaagagc agcaagacct tcttcctgac ctcctacgag cggcgctggg acttcgcgcc
      661 ggtcacgctg agggcgagcg acatgccgac ctcgacgatc ctgggcggcg acttccgcgg
      721 gctggccgag acgacccgcc cggcggtgcc ggccgagatc ctcccgctgt tgacgccgga
      781 ggagctggcg aacaacacct tcctgacggg gacgacccgg cgcttcaacg ttatcccgga
      841 gcgtttgctc aatcccatcg cgctcagcct cgttaacacc tactacccgc agaccggcaa
      901 cgcgcctttc aacccggtga acggacgcct catcgacttc taccagaaca tcccggggct
      961 catcacccgc gacctgttca ccggcagaat cgaccacgac ttctccgaca aggacaagtt
     1021 ctacgcagtt tacaactacc agaagcggaa cggcgtccgc gcgctgacgg cgaacctgcc
     1081 cgccttcggc ctgcgcgagc aagagggcgc caatcacaca ctcgccctct cttacacgag
     1141 ggtcttctcg cccaccgttg tcaacgaggc gcgcggcggc ttcaacaccc agaaccttta
     1201 cacgcacgcc cggcagacga cgcaggagtt cctcgccggc ctgggcttca acgatcagga
     1261 gatcgcctcc tacggctcga tcgttggccc gagcctcctc gacacgttcg ggcacctcgc
     1321 cttcaccctc ggctctttcc ggggcatcgc ctccggcgcg cgtaacgccg accgctcatt
     1381 cgaccagagc ctgatcacct tcggcgatac ggtgaccatc atcaccggtc agcacacgat
     1441 caagggcggc gccgacttca tccgcaacca ggccatcgac gacttcgtca agaaccgcaa
     1501 cgacccgcgc gggacgatca tctacccgaa cagcttcgcc ggtttcgcca ggttcttgat
     1561 cggcctcccg ccctcgagcg tccgctacgt cttcaacgtg cgcccctcga tggacgtgca
     1621 caactgggag accggcttct tcattcagga cgactggaag ctccactcgc gcctgacctt
     1681 gaacctcggc ctgcgctacg acctgatcac cccgttcgtt gacaagaacg acttgatgat
     1741 caacttcgat ccgaacgcga cgggaaacaa cggcaacaag ggccgcttca tcgtgcccag
     1801 cgccgacatc atcccgacgc tcctcccggc cttcgtctcc tacggcgtcg tgacggctga
     1861 ggaggccggc gtcgggcgcg gcctcatcga ggtagaccgc aacaacctcg cgccgcgcct
     1921 cggcctcgcc tggcgcgtga cggataacac cgtcatccgc ggcggcttcg gcatgttcta
     1981 cccgacctcc gccgcgcagg ggcagcgcga cgccttcgcc tcgacggcct tcaaccagcg
     2041 catcaccaag aactcgacga ccgccgcgcc gctcgccggt ctgcccggcg gcatcaaccc
     2101 gcggggcctc acccccttca gcggcggcac cttcgccgtg cagggtctct ccgtcaacgc
     2161 catcccgacc gacctccaaa gcccgcgcat cgaccagttc aacgtgacgg tcgagcgcga
     2221 cctcggctgg aagacgggct tgcgcgtctc gtacctcggg agccgcatgc acggactgat
     2281 cgccggcacc gacctcaacc tgctcccgcc gagcgacatc ccgttcggca cgacgaccgg
     2341 cgacggcgtc acgccgtgcg tccccaacga cttcaactgc cagatttcag ccgccgaccg
     2401 ggcgcggctg cccttcccgc tgctcggcga cttcctcgcc agctacggca acatcggcag
     2461 cggcaagtcc gacgcgctac agatcgaggc gaaccgccgg ttcgacaacg ggctgacctt
     2521 caacgtcagt tacaccctgc tcgaccagaa gagcagcggc ctcgacgtgg gcgcttccag
     2581 cctcggcggc accctctata accagttcaa ccccgagggc gactacagcc gcgacggctt
     2641 cgtctcgcgg caccgcgtcg tctactacgg cgcctacgat ttgccattcg gtcgcgggcg
     2701 ccggttcggc agggagtcgt cgcgcgtcct cgacgccgtg gcgggcggat ggcaactcac
     2761 ctggaacggg ttcgccaaga gcggcaccgg gttcacgccg ttctggacat gcacgaactg
     2821 tagcccggcc ttccccggca acatcgccag ctcctttatc gacccggtcg ggggcttcgg
     2881 cggcgtcgtc ggcttccgcg ccacggtggt gtcgagtgcg gaaccgtatc tccggcaggg
     2941 ggatcagttc ttcaaccccg ccgccttcgg gattccgtcg gcgggcgccg acgtgctcga
     3001 caacccggcg ggcgcgaagc gcaacttcct gaccgggccg ggcacatggg gcgccaacct
     3061 cggcctgcgc aagtacttca agttctcgga gacgacgagg ctcgaagtgg gggccgattt
     3121 caacaacatc ttcaaccacc cgctcaagtc gcctcaggac ccctacttcg cgagggtcgg
     3181 cgagttcgcc atcgccgttg acccggcgac gaagaaggtc atcatcccgg cctcgggcgt
     3241 cactcgcaac ccgctgttcg ggcggacgat tgacagcttc cggcaggagg gcatcgagga
     3301 ccgccgcctg attcgcgtga aactacgcct gaccttctag gccgggccac acaaccggga
     3361 aatgaagaag gaggggcgac ggttgatccc tcatccctcc ttctctattt ccctaactcc
     3421 gcttctttcg gtaggctcgt tcccgtagta gggctgcgcc aaacaggggc ttcagaggat
     3481 gagtagcgaa gaccgcacac ttcaggtttc cacccgcaaa ccgaacctct ccaacgtcaa
     3541 ggtcgcgggg catgatgtgc tccgcacggt caaccgtcgc atcctcctca acatcctctc
     3601 cgagcgccag cccatctcgc gcgccgacat cgccaaactg agcggcctca acaaggcgac
     3661 cgtctccgcc atcaccagcg cgctgctcaa ggatcacttc atcgtcgagg agggttcggg
     3721 gcgcacgacg cccatcggcg gcaagccgcc cacgcccttg cgcctcaacg agctacggtt
     3781 cggcatcctg ggtctcgacg ttcgcgccga cgaaaccatt cttgcactga gcgatttcca
     3841 taaccgtctc gtcgcgcgca agagctacga gacgggcaac gacccgcgcg cctttctcac
     3901 ggagatcggc ggcgagatca cgcgcctgcg cgccgagcac acccagttca tcgaggtcgc
     3961 gggcatcggc gtcagcctcc ccggactcgt cgataaccgg gcggggaagt tcatctcctc
     4021 ggtcgtgctt ccctggcgcg acgtgcccgt cgtcgagtgg ctccgcgagt cgacgcgcct
     4081 gcccgtcatc atcgacaact cggcgcggtg cgccgccctc gccgaaatct ggcacagcaa
     4141 atcgcactac gcgcacgtcc gcaacctcct ctacgtgagc gtcagcagcg gcctcgcctg
     4201 cggcgtcatc atcgacggca gcctctaccg cggctggacg aacgccgcgg ggcagttcgg
     4261 ccacatcccc atcgacttca acggccccga atgtcgctgc gggcagcgcg gctgctggga
     4321 cctctacgct tccgacaacg ccacggtcgc ccgctatcga aacctccccg gcgcgaccgc
     4381 acggcgcggc gtcacgatga ggaagatcgt cgagctggtc gagtcgggcg acccggcggc
     4441 caccgagacc gtgcgcgaga cggcgcgcta cctcggcatc ggcatcgccg gcctcgtcaa
     4501 cgggcttgac cccgaggtcg tcgccatcgg cggcgagatc gcgaaggcgt ggggcgtggt
     4561 cgagccgatc atcgcgcggg agatgggccg caacatgctc gcctccgcgg ggcgcaagat
     4621 agagctgcgg cgctcgacct tcgacgtgcg cccgagtctc aagggcgcgc tcacgctcgt
     4681 cctcagcgag ctgctgtccg tgccgcacct cgggtagggg cgcgagaagg aaatgaccca
     4741 gaccgcagaa cggattcgcg cacgactcga cgggggcctc atccccgccg tccccgtgac
     4801 gttggacgag cgcgggagaa tccacgcgcg cgcgcatgat gcgtacctgc gccacatgag
     4861 cgaccagccc cacgcgggcg tcgccgtctg ggcgcacacg ggccgcggct tgatgctcga
     4921 ccgcgagatg gccgacgcgg tgctcaagga ctggcgcgcg gcgctccccg gcggagtcgt
     4981 cgtcgccggc gcgggcgccc ccccgaccgc cgacccttcg gaggcgaccg accgcgccgt
     5041 cgagatggcc gagcgcgccg cacggctcgg cgcggacgcg ctgctcgcct acgcgcccgc
     5101 gtggctgcgt ggccgtgaag accgcgaccg ccggatcgtc gagcaccacc gaaggctcgc
     5161 ccgcgtgggc ctgccgctcg tgctcttcta cctctacgaa gaggcgggcg gcgtcgg
//
LOCUS       DBNGOH010000081         2848 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_195410, whole genome shotgun sequence.
ACCESSION   DBNGOH010000081 DBNGOH010000000
VERSION     DBNGOH010000081.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2848)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2848)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2848
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_195410"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1743
                     /locus_tag="AC159P_01565"
     CDS             <1..1743
                     /locus_tag="AC159P_01565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694167.1"
                     /translation="FFVPAGYLFFLIRRYGVDVPFLEQWNVALLFEKLSHGTLTVADL
                     FAQQNEYRQFFPHVLLVGLGWLTDWNVKYDLYLIFLCACLISVGVYRLGALTLGGTGE
                     VRRAAAYFFTNLLIFSPMQSENWLYGIQLVYLLPVACVVAGVLVAYAGAGLRVKFAAC
                     AALSVVATFSAANGVLSWLVLLPLLLLSGPRGGLKEWKWLAPAWVALMALSVAVYLHG
                     LRATPEHPSTFETLRHPVRAALFFFSFLGTPLWAGSLWVTVAAGLALALVYAPACAYL
                     WRNRSDRALVRRLLGWLMLGAFSLLTGVLVTLGRSGFGVQQSETARYIAFSLYLAAAV
                     AHVVPIIAVELEKQGSGSRERARFVRRAAALALALALVAQARLYPIGTLSMMYKWREH
                     AHVKVCTLFVNVLPDACPTARLLPEGLLRRGANALDAIGYLRPSLIRGGRLEEIEAQG
                     AAGEFEELREEEGGTLVASGRARLPGGGRPADAVLLAYEKGEAGSFVFALSEPHAGKE
                     YAIRRLREVARPDLRWRARVPAGALPGGPIKITAWAFDAEEGKAYRLGGFHVAPPPRM
                     TRARAPRPACSRHV"
     gene            1740..2456
                     /locus_tag="AC159P_01570"
     CDS             1740..2456
                     /locus_tag="AC159P_01570"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012191427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR3694168.1"
                     /translation="MREGISVFFPAYNDAGTIAGLVEEALAVLPSLTDDYEVIVVDDG
                     STDATGAVLDELALREPRLRVIHHEQNRGYGGALRTGFGAARKDLIFYTDGDGQYDVR
                     ELAALGPLMKEGVDIVNGYKIKRADGLDRKIIGGVYNRLARLLFRLPIRDVDCDFRLM
                     RRRAVQQLDLVSSSGVICVELAHKLRRAGCAFAETPVNHYPRRHGRSQFFTAPRVART
                     ARDFFALWFKLVLLRRGVKP"
     gene            2745..>2848
                     /locus_tag="AC159P_01575"
     CDS             2745..>2848
                     /locus_tag="AC159P_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase"
                     /protein_id="HIR3694169.1"
                     /translation="MSKTFGAYRGRSVLITGGLGFIGSNLARKLVEVG"
BASE COUNT          399 a          899 c         1016 g          534 t
ORIGIN      
        1 tttttcgtgc ccgccggcta cctgtttttt ttgatccgca ggtacggcgt tgacgtgccg
       61 ttccttgagc agtggaacgt cgcgctgctc ttcgagaagc tgtcgcacgg gacgctcacc
      121 gtcgcggacc tgttcgcgca gcagaacgag tacaggcagt tcttcccgca cgtcctgctg
      181 gtcggcctcg gctggctcac ggactggaac gtcaagtacg atctctatct catcttcctg
      241 tgcgcgtgcc tgatctcggt cggcgtctac aggctcggcg cgttgacgct cggcgggaca
      301 ggcgaagtgc ggagggccgc ggcgtacttc ttcacgaacc tgctgatctt ctcgccgatg
      361 cagtccgaga actggctgta cgggatacag ctcgtgtacc tgttgccggt ggcgtgcgtc
      421 gtcgcgggcg tgctcgtcgc gtacgcgggc gcgggcctgc gggtgaagtt cgcggcgtgc
      481 gcggcgctct cggtggtcgc cactttctcg gcggccaacg gggtgttgag ctggttggtg
      541 ctgttgccgt tgctgctcct gtcggggccg cgcgggggct tgaaggagtg gaagtggctg
      601 gcgcccgcgt gggtcgcctt gatggcgctg agcgtggcgg tctacctcca cggtctgcgt
      661 gcgacgccgg agcacccgag cacgttcgag acgttgcgcc atcccgtgcg ggcggcgctc
      721 ttcttcttct ccttcctcgg gacgccgctg tgggcggggt cgctgtgggt gacggtcgcc
      781 gccggactcg cgctcgcgct cgtctacgcg ccggcctgcg cttacctgtg gaggaacaga
      841 agcgaccgcg cgctcgtccg acgcctgctc gggtggttga tgctgggtgc gttctcgctc
      901 ttgacgggcg tgctggtcac gctcggcagg agcgggttcg gcgtccagca gtcggagacg
      961 gcgcgctaca tcgccttctc gctctacctc gcggcggcgg tcgcgcacgt cgtgccgatt
     1021 atcgcggtcg agttggaaaa gcagggaagc ggctcgcgcg aacgcgcgcg attcgtgcgg
     1081 cgggcggcgg cgttggcgct cgcgctggcg ctcgtcgcgc aggcgcggct ctaccccatc
     1141 gggacgctct cgatgatgta caagtggcgc gagcacgcgc acgtgaaggt gtgcacgctc
     1201 ttcgtcaacg tgttgccgga cgcctgcccg acggcgcggc tgctgccgga aggtctcctg
     1261 cggcggggcg cgaacgcgct cgacgcgata ggctacctgc ggccttcgtt gattcgcggc
     1321 ggcaggctcg aagagatcga ggcgcagggc gcggcgggcg agttcgagga gttgagagag
     1381 gaagagggcg gcacgctcgt cgcctccggt cgggcgcgtt tgcccggcgg gggacggccc
     1441 gccgacgcgg ttctgctcgc ctatgaaaag ggcgaggccg ggtcgttcgt cttcgccctg
     1501 agcgagccgc acgccgggaa agagtatgcg attaggcgtt tgagagaggt ggcgcggcca
     1561 gaccttcgct ggcgcgcgcg tgttcccgcc ggagcgcttc cgggcggccc catcaagatc
     1621 accgcgtggg cgttcgacgc cgaagagggg aaggcttaca gactcggcgg attccacgtc
     1681 gccccgcccc cgcggatgac gcgcgcacgg gcgccgcgac cggcgtgcag cagacacgta
     1741 tgagagaggg catcagcgtt ttcttccccg cgtacaacga cgcgggcacc atcgcggggc
     1801 ttgtcgaaga agcgctcgcc gtcctgccgt ctctgacgga cgactacgag gtcatcgtcg
     1861 tcgatgacgg gagcacggac gcgacgggcg ccgtgctgga cgaactcgcg ctgagagagc
     1921 cgcggctgcg cgtgatccat cacgagcaga accgcggtta cggcggggcg ctccgcacgg
     1981 gcttcggcgc cgcgcgcaag gacttgatct tctacacgga cggcgacggg cagtacgacg
     2041 tgcgcgagct ggcggcgctc ggcccgctga tgaaggaagg ggttgacatc gtcaacgggt
     2101 ataagataaa acgcgcggac ggactcgacc gcaagatcat cggcggggtc tacaaccggc
     2161 tggcgcgcct cctcttccgt cttcccatcc gagacgttga ctgtgatttc cggctgatgc
     2221 ggcgccgcgc cgtgcagcag ctcgacctcg tctcttcgag cggcgtcatc tgcgtcgagc
     2281 tggcgcacaa gctgcggcgc gcgggctgcg ccttcgccga gacgcccgtc aaccactacc
     2341 cgcggcggca cgggcgctcg cagtttttca cggcgccccg cgtggcgcgc acggcgcgcg
     2401 actttttcgc gctgtggttc aagctggtct tactgcggcg cggcgtcaag ccgtgagctt
     2461 ccgccaagcg gtttagagca agtttcagac gggtgcggtg tgtggtgaag gttgtagggg
     2521 cagggcaagt ccgcggcccg ccgttcgact gattcaacgt tgagtgctaa cgaatcttga
     2581 gcgggccgcg gacttgcccc tgcccctacg gttcgcgcgc gttctacagt caactgaaac
     2641 ttgctctaag aattccccgc aaaagttttc ttcgttcttc gatcacatcc cgacaggtcg
     2701 ggccgctccg aggcgccgcc gaggctttgc aacgaacgca tatgatgtcg aagaccttcg
     2761 gcgcgtaccg tggccgctcc gtgctcatca cgggcggtct cggcttcatc ggcagcaacc
     2821 tggcgcgcaa gctcgtcgag gtaggcga
//
LOCUS       DBNGOH010000082         2528 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3678303, whole genome shotgun sequence.
ACCESSION   DBNGOH010000082 DBNGOH010000000
VERSION     DBNGOH010000082.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2528)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2528)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2528
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3678303"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            170..898
                     /locus_tag="AC159P_01580"
     CDS             170..898
                     /locus_tag="AC159P_01580"
                     /EC_number="1.5.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014096308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HIR3694170.1"
                     /translation="MRVSLFITCLVDQLWPSVGASAVEVLRRAGCEVVFDERQTCCGQ
                     PAFNTGYRTEARRLAERFIEIFEESGSDAVVSPSGSCTAMAHHFHELFDRDAGWRRRA
                     EALAARTHEFGSFLVNVLGVEDVGASFRGRVTWHDACHGLRDLNVHGEPRKLLRSVRG
                     LEFVELANADSCCGFGGTFSVKYPEISVAILDQKIEAIEQAGVRAVVSGDASCLMQIG
                     GRLTRGGSRVRAMHLAEVLAAKED"
     gene            910..2403
                     /locus_tag="AC159P_01585"
     CDS             910..2403
                     /locus_tag="AC159P_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HIR3694171.1"
                     /translation="MEHQTSETFDRNAREALRDPQLRGALRNLATTIGERRLTAITTV
                     EDWEGLRERARAVKDETLAHLDKYLEAFADNAERAGARVHWARDAGEACAIVIRLVQE
                     RQATHVVKSKSMATEEIHLNAALEAVGLEPVETDLGEWIIQLAHETPSHIVVPAIHKS
                     KEQIAELFVEKVGIEPTDDVAVLTQTARRILRERFAEAEVGISGVNFGVAETGTILIL
                     ENEGNARLTTSLPRTHIAVMGIEKVIPRFTDLDVFLKLLPRSGTGQRLTAYQSLITGT
                     KRGADEEGPEEVHIVLMDNGRSRMLSHPVTRQSLACIRCGACLNACPVYQQVGGHAYG
                     SVYPGPIGAVITPQLVGIGKAAQLPYASSLCGACREVCPVKIDIPQLLLHLRAEVAEG
                     TASGTATQKGPVKRKFLERLAFRLYAFAASSQRLYEWGARVGRVLQRPAVRDGRIGKV
                     SGLVAKLAPPLAAWTEWRDAPPLAARSFREEWRDGLSRSKPGESDAA"
BASE COUNT          405 a          813 c          910 g          400 t
ORIGIN      
        1 ccgacgaagt gatcgtcgaa ctcgacaagg cgttcggatt tagttaacgg gtgttgggtg
       61 ttgggttgtc gccctgcgca agctcgaagc cggatttcaa agcccagcct tcgtgtccga
      121 aaaacccagc acccagcacc cagcacccaa caccctttga ggatttcgtt tgcgcgtctc
      181 actcttcatc acgtgtctgg ttgaccagtt gtggccttcg gtcggcgcga gcgcggtcga
      241 ggtcttgcgg cgcgcgggct gcgaggtcgt cttcgacgag cggcagacgt gctgcggcca
      301 gcccgccttc aacaccggct accgcacgga ggcgcggcgg ctggccgagc gcttcatcga
      361 gattttcgaa gagtcggggt cggacgccgt cgtcagcccg tcgggttcgt gcacggcgat
      421 ggcgcaccac ttccacgagc tgttcgaccg ggacgcgggc tggcgccgcc gcgccgaagc
      481 gctcgccgcg cggacgcacg agttcggctc gttcctcgtc aacgtgctcg gcgtcgagga
      541 cgtgggcgct tccttccgcg ggcgcgtgac gtggcacgac gcgtgtcacg ggctgcgcga
      601 tctgaacgtc cacggcgagc cgcgcaagct cttgcgcagc gtgcgcgggc tggagttcgt
      661 cgagctggcg aacgccgact cgtgctgcgg ctttggcggg acgttctcgg tgaagtaccc
      721 agagatttcc gtcgccatcc tcgaccagaa gatcgaggcc atcgagcagg cgggcgtgcg
      781 cgccgtcgtc tcgggcgacg cgagctgcct catgcagatc ggcggtcgcc tgacgcgcgg
      841 cggctcacgc gtgcgcgcga tgcacctcgc ggaggtgctg gcggcgaagg aggattgagc
      901 ggcacgccga tggaacacca gacctcggag accttcgacc ggaacgcgcg cgaggctttg
      961 cgcgacccgc agttgcgcgg ggcgctgagg aacctcgcga cgaccatcgg cgagcggcgc
     1021 ctgacggcca tcacgacggt cgaggattgg gaggggttgc gcgagagagc gcgcgccgtg
     1081 aaggacgaga cgctggcgca cctcgacaag tacctcgaag cgttcgcgga caacgccgag
     1141 cgcgcgggcg cgcgcgtcca ctgggcgcgc gacgcgggcg aagcgtgcgc catcgtcatc
     1201 cggctcgtgc aggaacgaca agcgacacac gtcgtcaagt cgaagagcat ggcgaccgag
     1261 gagattcatc tcaacgcggc gctcgaagcc gtgggccttg agccggtcga gacggacctc
     1321 ggcgagtgga tcatccagct cgcgcacgag acgccttcgc acatcgtcgt gcccgcgatt
     1381 cacaagtcga aggagcagat cgccgagctg ttcgtcgaga aggtcggcat cgagccgacg
     1441 gacgacgtgg cggtgctcac gcagacggcg cggcgcatcc tgcgcgagcg cttcgccgag
     1501 gccgaggtcg gcatctccgg cgtcaacttc ggagtggcgg agacgggcac gatcctgatt
     1561 ctcgaaaacg agggcaacgc gcgcctgacg acgagcctgc cgcggacgca catcgccgtg
     1621 atgggcatcg agaaggtcat cccgcgcttc accgacctcg acgtgttctt gaaactgctg
     1681 ccgcgttcgg ggacgggcca gcgcctgacc gcctatcaat cgctcatcac gggcacgaag
     1741 cgcggcgcgg atgaagaggg gccggaggaa gtccacatcg tcctgatgga caacgggcgc
     1801 tcgcggatgc tgtcgcaccc ggtgacgcgc cagtcgctcg cgtgcatcag gtgcggcgcg
     1861 tgtctgaacg cgtgcccggt ctaccagcag gtcggtggcc acgcctacgg ctcggtgtat
     1921 cccggcccca tcggcgcggt catcacgccg cagctcgtcg gcatcggcaa ggcggcgcag
     1981 ctcccttacg cttcgagcct gtgcggcgcg tgccgcgagg tgtgcccggt caagatcgat
     2041 atcccgcaac tgctcctgca cctgcgcgcg gaagtcgcgg aagggacggc ttcggggacg
     2101 gcgacgcaga aggggccggt gaagagaaag tttcttgagc ggctcgcgtt ccggctctac
     2161 gccttcgccg cttcgagtca acggttgtac gagtggggcg cgcgcgtcgg gcgtgtgctg
     2221 caaagaccgg cggtgcgcga cgggcgcatc ggcaaggtgt cgggcctcgt tgccaagctc
     2281 gcgccgccgc ttgccgcgtg gacggagtgg cgcgacgcgc cgccgctcgc cgcgcgctcg
     2341 ttccgcgagg agtggcgcga tgggttgtcg cggtcaaagc ccggcgaatc cgacgccgcg
     2401 tagcggcgat tgaatgtagc ccggcgttga aacgccgggt cagggactca cgacgcggcg
     2461 tcgcgtcagc gacgcttgac ggatgactca ggcgtcgctg acgcgacgcg gttatgtggt
     2521 cgcctcgc
//
LOCUS       DBNGOH010000083         3095 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_195510, whole genome shotgun sequence.
ACCESSION   DBNGOH010000083 DBNGOH010000000
VERSION     DBNGOH010000083.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3095)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3095)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3095
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_195510"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1931
                     /locus_tag="AC159P_01590"
     CDS             <1..1931
                     /locus_tag="AC159P_01590"
                     /EC_number="2.7.7.65"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024878.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphohydrolase"
                     /protein_id="HIR3694172.1"
                     /translation="FLLASGLFLSGLCVSYALDKSLPVVVALNAPALCLYFLLSRQHI
                     RRLEEAIAAAARKLHEVEETAGVRLRTVEALAAEIDAKDQTPRGHVRRTRVYAVETGR
                     LLGLGERELAALGDGALLHDVGKLAVPEYVLNKPDKLTAAEFERIKSHTTVGAALLDE
                     VGFPYPVEDVVLYHHERWDGGGYPAGLKGEQIPLVARIIAVVDFYDSTRCDRPFRAGM
                     SRHESLSLLERRAGTAFDSRVVETFVRHVEEFDKLIDAADLREQAMGATNVEPAKSTY
                     LHSPAPCARGEAEGPAGFRSISEAQREVFALHEIAHTVGRSLDLEDTSDLISGKLRAV
                     MPFDTCVIHILDEKSGCAYAAHVAGADEDYFSSRRVPAGRGVTGWVISNARSMRSASP
                     ELDLADAPDELRSRLRGVIAAPLVRDGAAYGAVALYSSDETPYTSEHLRLLETVALYA
                     SNAISNSLTFVRTRENALADPLTGLQNERALRLLLEQRLAECRRRPDAPLAVLSLDID
                     DLGGVNGVHGHGVGDRLIAEVADVIKAQLRQMDTLARCEADEFVAVMPTANAEAAGTV
                     AERIRSAVEAHRFTVRAGLTLRVGLSVGVACFPARGETANDILLAAHADMERNKHIRK
                     FESHGSGPGEVIRLDSYR"
     gene            2192..>3095
                     /gene="pdhA"
                     /locus_tag="AC159P_01595"
     CDS             2192..>3095
                     /gene="pdhA"
                     /locus_tag="AC159P_01595"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - pyruvate decarboxylation to
                     acetyl-CoA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HIR3694173.1"
                     /translation="MSELKVTPMKPRPHPDEEAGHAVEAREEAGQEPPSSLREEHDQR
                     KRAQQEQIEETDRDTLHRMLYQMILIRRFEEKAAEAYTLGKIGGFCHLYIGQEACAVG
                     ALSALRPDDHVLTSYRDHGQALIRDTSPDEVMAELFGKAGGCSAGKGGSMHLFNAEAN
                     YHGGHGIVGGQIPLATGIAFATKYRETDQVTLCFFGEAAVNQGAFHESLNMAQLWKLP
                     CIYIVENNQYGMGTAIKRAMSRSAVWEKAAAYDIASEFVDGMDVLAVRQATLRAVERA
                     RKQYLPSLLEIQTYRFMGHSMSDPG"
BASE COUNT          519 a         1089 c         1009 g          478 t
ORIGIN      
        1 tctttctcct cgcgtccggt ctctttctct ccggcctctg cgtctcgtac gcgctcgata
       61 aatcgctgcc ggtcgtcgtc gccctgaacg ccccggccct ctgtctctat tttctgttgt
      121 cgcgccagca cattcgccgt ctcgaagaag ccatcgccgc cgcggcccgc aaacttcatg
      181 aagtggaaga gaccgccggt gtgcgtctga ggacggtcga ggcgctggcc gcggagatcg
      241 acgccaaaga ccagacgccg cgcggccacg tccgccgcac gcgcgtctac gccgtcgaga
      301 cgggcagact gctcggcctc ggcgaacgtg agcttgcggc gctcggcgat ggcgcgctgc
      361 tgcacgacgt gggcaagctg gccgtccccg agtacgtgct gaacaagccg gacaagctga
      421 ccgccgccga gttcgagcgg atcaagtcgc acacgacggt cggcgccgcg ctgctggacg
      481 aagtcggctt cccttacccg gtcgaagatg tcgtgctcta ccaccacgag cggtgggacg
      541 gcggcggcta ccccgcgggg ctgaagggcg agcagattcc gctggtcgcg cgtattatcg
      601 ccgtggtcga tttctacgac tcgacccgct gcgaccgccc cttccgcgca gggatgtcgc
      661 gacacgaatc gctctcgctc ctcgaaagaa gggcgggcac cgctttcgat tcgcgggtgg
      721 tcgagacgtt cgtccgccac gtcgaagagt tcgacaaact gatcgacgcc gcagacctcc
      781 gagagcaggc gatgggagcg acgaacgtcg agccggcgaa gtccacgtat ctccattccc
      841 ccgctccctg cgcgcggggc gaagccgaag gcccggctgg cttccgctcc atctccgagg
      901 cgcagcggga ggtgttcgcg ctccacgaaa tcgcgcacac cgtcggcagg tcgctcgacc
      961 tcgaggacac gtccgatctg atctccggca agctgcgcgc ggtcatgccc ttcgacacgt
     1021 gcgtcatcca catcctcgac gagaagagcg gctgcgctta cgcggcccac gtcgcgggcg
     1081 ccgacgagga ttacttctca agccgccgcg tccccgccgg gcggggcgtg acgggctggg
     1141 tcatctccaa cgcgcgctcg atgcgcagcg cctcgcccga gcttgacctc gcggacgcgc
     1201 cggacgagtt gcgcagccgc ctccgcggcg tcatcgcggc gcccctcgtc cgcgacggcg
     1261 cggcctacgg ggccgtcgcg ctctactcct cggacgagac gccctacacc tcggaacacc
     1321 tgcggctgct ggagacggtc gcgctctacg cgtcgaacgc gatcagcaac tctctgacgt
     1381 tcgtcaggac gcgcgagaac gcgctcgccg acccgctcac gggtttgcag aacgagcgcg
     1441 cgttgcggct cctgctcgaa caacgtctgg ccgagtgccg ccgccgaccg gacgcgccgc
     1501 tcgccgtgct ctctttggac atcgacgacc tcggcggcgt caacggcgtg cacggccacg
     1561 gcgtcggcga ccgcctgatc gcggaagtgg cggacgtgat caaggcgcag ttgcgacaga
     1621 tggacacgct cgcgcgttgc gaggccgacg agttcgtcgc ggtcatgccg acggccaacg
     1681 cggaggcggc ggggacggtc gccgagcgca tccgctccgc cgtcgaggcg cacaggttca
     1741 cagtccgcgc cggcctgact ctgcgggtcg gcctcagcgt cggcgtcgcc tgcttccctg
     1801 cgcgcggcga gacggcgaac gacatcctgc tggccgcgca cgccgacatg gagcggaaca
     1861 aacacatccg caagttcgaa tcgcacggct ccggccccgg cgaggtgatt cggctcgact
     1921 cgtacaggta gcgaagctca agcttcgagg ttcgaagtcc gaggcgcaag gatggagatg
     1981 cgttcttcaa tcttgcgcct cgaacttcaa tgtccgaact tcgaacctcg aacttcgaac
     2041 ctcgcctttt tgcgcgacgg ctgcccgttc gcgtataatc cctccccgta atacccggac
     2101 aattttttac accccgagag agcgctcccg ccaacgggcg cccgccgaag aacgcgccgg
     2161 gccgggccgc gggcgctgag agattattgc catgagcgag ctgaaagtga cgccgatgaa
     2221 gccccgccca cacccggacg aagaggccgg ccacgcggtc gaggcgcgtg aggaggcggg
     2281 gcaggagccg ccgtcgagcc tccgcgaaga gcacgaccag cggaagcgtg cgcagcaaga
     2341 gcagatcgag gagaccgacc gcgacacgct ccaccgcatg ctctaccaga tgatcctcat
     2401 ccgccgtttc gaggagaagg ccgcggaggc ttacacgctc ggcaagatcg gcggcttctg
     2461 tcacctctac atcgggcagg aggcgtgcgc cgtcggcgcg ctgtccgcgc tcaggcccga
     2521 cgaccacgtg ctcaccagct accgcgacca cggccaggcg ctcatccgcg acacctcccc
     2581 ggacgaggtg atggccgagc tgttcggcaa ggcgggcggc tgctcggcgg gcaagggcgg
     2641 ctcgatgcac ctcttcaacg ccgaggccaa ctaccacggc gggcacggca tcgtcggcgg
     2701 ccagataccg ctggcgacgg gcatcgcctt cgccacgaag taccgcgaga ccgatcaggt
     2761 cacgctctgc ttcttcggcg aggcggcggt caatcagggc gccttccacg agtcgctcaa
     2821 catggcgcag ctctggaagc tgccgtgcat ctacatcgtc gagaacaacc agtacgggat
     2881 gggcacggcc atcaagcgcg cgatgtcgcg ctcggcagtc tgggagaagg ccgccgccta
     2941 cgacatcgcc tccgagttcg tggacggcat ggacgtgttg gccgtgcggc aggccacgct
     3001 gcgcgcggtc gagcgcgcgc gcaagcaata cctgccgtcg ctgctcgaaa tccagaccta
     3061 ccgcttcatg ggccactcga tgtccgatcc cggcc
//
LOCUS       DBNGOH010000084         1831 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4961578, whole genome shotgun sequence.
ACCESSION   DBNGOH010000084 DBNGOH010000000
VERSION     DBNGOH010000084.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1831)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1831)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1831
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4961578"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1831)
                     /locus_tag="AC159P_01600"
     CDS             complement(<1..>1831)
                     /locus_tag="AC159P_01600"
                     /inference="COORDINATES: protein motif:HMM:NF012242.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HIR3694174.1"
                     /translation="TENDFAAGGARLPWEEQAEGAVVGVLAREQGALVPGRQVSSAKS
                     WLCLSSVDRTADILPWGAQPDERSCSPVAASARYLAHLRDAWNHSVARRGDGAAADDL
                     RFERQTIVLTVPASFDEEARELTVEAARAAGLENLTLLEEPLAAFYAWIAAHRTALKR
                     QLKDGQLVLVCDVGGGTTDFSLIRVSVEGKEVRFERTAIGEHLLLGGDNVDLALARRV
                     EEKLGGPRLTVRQQQSLRRQCCAAKEKLLSDASIERVPVTVLGGGRSLVGGAMTTELT
                     REEVEAVLSEGFLPLTATGDLPAHAQRVALRELGLPYATEAAITRHLAAFLTQAASAM
                     DEDLRGTHVDEASAVANVEANAANDANAEDEPAKATGGERFEGMVRPDAVLFNGGFFH
                     PPLTRERVVAAIAGWFPRAGKRWRPKVLNSETPETAVAVGAAYYAQARATGGLRVSGG
                     SARGYYIGVQHTEARGAGEGGGRVTAVCVLPRGTEEGTRLELAEREFTVLANRPVSFT
                     LYSTTQRHDAQGAVVQLEEEEAHRHAPLITVLRYGKRSRHVELDERLTAHFTEVGTLE
                     LWCEAPKTGHRWRLQFQLRGAVAAEDEEAPPEEAQAVEEGSQVF"
BASE COUNT          239 a          696 c          605 g          291 t
ORIGIN      
        1 cgaagacctg cgacccctct tcaaccgcct gcgcttcttc cggcggcgcc tcttcgtctt
       61 ccgcggcgac cgcgccgcgc agttggaatt ggaggcgcca gcggtggccc gtcttcggcg
      121 cctcgcacca gagttcgagc gtgccgacct ctgtgaagtg cgccgtcaaa cgctcgtcca
      181 gctcgacgtg gcgcgagcgc ttgccgtagc gcagcacggt gatgagcggc gcgtggcggt
      241 gcgcctcttc ctcttcgagc tggacgaccg cgccctgcgc gtcgtgccgc tgcgtcgtgc
      301 tgtagagcgt gaacgagacg ggacgattgg cgagcaccgt gaactcgcgc tcggcgagtt
      361 cgagccgcgt cccttcctcc gtcccgcgcg gcagcacgca gaccgccgtc acgcgcccgc
      421 cgccttcgcc cgcgccgcgc gcttccgtgt gttggacgcc gatgtagtag ccgcgcgcgc
      481 tcccgccgct gacgcgcagc ccgcccgtcg cccgcgcctg cgcgtagtag gctgcgccga
      541 ccgcgaccgc cgtctccggc gtctcgctgt tcaggacttt cggtcgccag cgcttgcctg
      601 ctcgcgggaa ccagcccgcg atggccgcga ccacgcgctc gcgcgtgagc ggcggatgaa
      661 agaacccgcc gttgaagagc accgcgtcgg gccgcaccat gccttcaaag cgttccccgc
      721 ccgtcgcctt cgccggttcg tcttccgcgt tcgcgtcatt ggcggcgttc gcttcgacat
      781 tcgcgacagc actcgcttca tcgacgtgcg tgccgcggag gtcttcgtcc atcgcggagg
      841 cggcctgcgt caggaaggcc gcgaggtgtc tggtgatcgc ggcctcggtc gcataaggca
      901 gaccgagttc gcgcagggcc acgcgctgcg cgtgcgccgg aaggtcgccg gttgccgtaa
      961 gcggcaggaa gccttcggac aagaccgctt cgacctcttc gcgcgtcagc tccgtcgtca
     1021 tcgcgccgcc gacgagcgag cgcccgccgc cgagcacggt gacgggcacg cgctcgattg
     1081 acgcgtcgct cagaagtttc tctttcgccg cgcagcactg ccgccgcaga gactgttgct
     1141 ggcggacggt gaggcgcggg ccgcccaact tctcttcgac gcggcgcgcg agcgcgaggt
     1201 ctacgttgtc gccgcccagg agcaggtgct cgccgatggc cgtgcgctcg aacctgacct
     1261 ccttgccctc gacgctcacg cgaatcaggc tgaagtcggt cgtgccgccg cccacgtcgc
     1321 agacgagcac gagctgcccg tccttcagtt gccgcttgag cgccgtgcgg tgcgcggcga
     1381 tccacgcgta gaacgcggcc agcggctctt ccaacagcgt caggttttcc agccccgccg
     1441 cccgcgccgc ctcgaccgtc agctcgcgcg cctcctcgtc gaacgacgcg ggcaccgtga
     1501 gcacaatcgt ctgccgctcg aaacgaaggt cgtccgccgc cgcgccgtcc cctcgccgcg
     1561 ccaccgaatg attccacgcg tcgcgcaggt gcgcgaggta gcgcgccgag gccgcgacgg
     1621 gcgagcacga acgctcgtcc ggctgcgcgc cccacggcag gatgtccgcc gtgcggtcaa
     1681 ctgacgacag gcacagccac gacttcgcgg acgagacctg tcgtcccggc acgagcgcgc
     1741 cctgctcgcg cgcgagcacg ccgacgacgg cgccttccgc ctgctcttcc cacggcagac
     1801 gcgcgccgcc cgcggcgaaa tcgttttcgg t
//
LOCUS       DBNGOH010000085         6496 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3128783, whole genome shotgun sequence.
ACCESSION   DBNGOH010000085 DBNGOH010000000
VERSION     DBNGOH010000085.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6496)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6496)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6496
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3128783"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            185..745
                     /locus_tag="AC159P_01605"
     CDS             185..745
                     /locus_tag="AC159P_01605"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR3694175.1"
                     /translation="MNSPEELVARARHGDEEAFRLIFDRYSRPVLGFVYDMVGQRELA
                     EDLTQETFVRAYKGLAGFRDEAKLSTWLFAIAKNVARESLRTRPREERKVEIDDPLVA
                     ELKDSRRSPAGALLDKELNEVVRKALERLDEDKRAVFTLKVFQQRSYEEIVEITGFSL
                     GKVKTDLHRARAEMRRRIGPYLGGEQ"
     gene            745..1824
                     /locus_tag="AC159P_01610"
     CDS             745..1824
                     /locus_tag="AC159P_01610"
                     /inference="COORDINATES: protein motif:HMM:NF024881.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor family protein"
                     /protein_id="HIR3694176.1"
                     /translation="MKCVDCLPLLEEFFDGEVDDKTNGRMRDHLAVCADCAEAFDALR
                     AEQEMFLRYDRELEISPALWQGVRAAIADERPRPERRPFLLRLRERLAAAAALFALRP
                     ALASSLALMAVGITAGLLWQAASQRPTGVAPTDIADSNGGARPAPSTDQTAITSTAPQ
                     VAQINDGETPDGDDEPGEVAPVKLDGEHVKPETAARVSSKSARVPAAPDAGDGIVRFL
                     DADHAITADDSLPSVASASQGGDDLADAAALLDPEDEAVARHVERAQVLLRSFKNTRA
                     AEGEGDLAYERELSRKLLEENITLQLDADADGNKKAKKVLSQLEPYLTDISNLRDRPE
                     REQLRSIKERMQKQEIIAALHVYDD"
     gene            1896..3272
                     /locus_tag="AC159P_01615"
     CDS             1896..3272
                     /locus_tag="AC159P_01615"
                     /inference="COORDINATES: protein motif:HMM:NF025030.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HIR3694177.1"
                     /translation="MPTTPRTHPKSVARIAAGLVLGATLLAGGAAGVRGQQKFTPERE
                     MLNRLVQSMQNDAAAKAFVQGRDQINEGKWATAATTFGKYVADYPADKNVDAALYWLA
                     YAQEKQKKYGEADAVLAKLIKEHTKSAWKNDAEKLRLLVRSKLGTLYYTGNNPDSLLD
                     PQQAQRPDADCELKVVALQALCQADRGARCSTHVNDALRSNTTCLVLKEAAITYLGRY
                     GGQDALPALIQMARTEPNEKLRMRAIAALGRTGDERALDVLRELAMSPVYENESPTDS
                     AIHALAGFENARAAAILADVIINGKNIEARTHGAQLLSQRRGDDVVDQLFRIYDAASE
                     IEVKKYVLAGLGNRKDPRAIAKLIEVARNDPNEALRKQAIHSIPNRNDEQDLDVLIPL
                     YDSERNAELKEHILDAIGHYQNQRAYQKLMQVVRSNESLERRKKAIQMLSRSKDPAVL
                     KFLEDMLK"
     gene            3460..4617
                     /locus_tag="AC159P_01620"
     CDS             3460..4617
                     /locus_tag="AC159P_01620"
                     /inference="COORDINATES: protein motif:HMM:NF025030.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HIR3694178.1"
                     /translation="MLATLAFFLLMLTPPAAAPASPQDQRFVPVEGATLQARQEAAAS
                     RAAAASPQTPYWTAYSFDVRPGVATDFEWVGDDGTHIILDGSSAFAFDPKLETRNLGV
                     FVLRSNEGGGAVKRVEVYNLARGREYSGYPVYWAGRASNDESLNFLRGLAETAATRAD
                     DVAAAATRALALHDDRRVPSLLEAFARTSTSTRVRAQAVRWLGHTTADTAPAPPLLWE
                     LARSDREFVEVRRAAVGALGQRKDAESLGVLQSLYDSLAQRELKRSVLSAIGRNENRS
                     AAAGFIVRVAERETDVELRKRAVAQLGEIAGEQALGALAQRAAQTDADTELQKQAVNA
                     IARRPAAESVPLLIKIARTHQKPEIRKQAFLLLGRTNDPTAIGFLREVLTK"
     gene            4979..5470
                     /gene="def"
                     /locus_tag="AC159P_01625"
     CDS             4979..5470
                     /gene="def"
                     /locus_tag="AC159P_01625"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010962042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HIR3694179.1"
                     /translation="MAVLEIVEFPAKVLRGAGAPVEKFDEELGKLVADMFETMYAAEG
                     VGLAAQQVGVSLRLFVMDCEGIKLVAANPEIVSAEGEQDGEEGCLSVGKIHAPLRRAN
                     RVTLRAQDEHGDLYEREATGLAARCFLHETDHNDGKLFIDRLTPLKREMVKKRFQKMK
                     KWR"
     gene            complement(5625..>6496)
                     /locus_tag="AC159P_01630"
     CDS             complement(5625..>6496)
                     /locus_tag="AC159P_01630"
                     /inference="COORDINATES: protein motif:HMM:NF013056.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015851 - nucleobase transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HIR3694180.1"
                     /translation="TFIGLQNAGLVAAHPVTLVRLGDLDHRALLMLLGLAVIALLLKW
                     RSPLAYLAGIFVVTLAAWALGKVQAPPQLFSAPDFYSVFLKLDLLGALRLSLLPAIIA
                     VMFTDLFDSISTFIGVSQAAGMLDEEGHPRRLREGLIVDSWATLGAGLAGTSSGTAYI
                     ESTAGIEMGGRTGLTSVFTALCFLPCFFIAPLAGMVPPYATAAVLLMVGVSMFRSVAG
                     LAFDRLEDALPAFLTIILIPLTFSITQGILWGFITHASLYLLAGRAREVHPIMYVLAL
                     VSIGLLLIQHVKQ"
BASE COUNT         1214 a         2241 c         2120 g          921 t
ORIGIN      
        1 ctccgtgact tgggccgggc cgtggcgtat gctgcgccct gcgccccgcc ggatgtgagc
       61 cgtaactttt ctgaaacttt cgctttcctg cggtcactta ttgacggtgt tagagatgga
      121 gcgattggct ggcaacatgg ttaatctgga tgcggcacag ccgggcgcgt acactggggc
      181 cttgatgaat agtcctgagg aactggtggc gcgcgcccgt cacggcgacg aggaggcttt
      241 ccgcctcatc ttcgaccgct actcgcgtcc cgtcctcggc ttcgtctacg acatggtcgg
      301 gcagcgcgag ctggccgaag atttgacaca ggagaccttc gtccgcgcct acaaggggct
      361 ggccggtttc cgcgacgagg ccaagctctc gacctggctc ttcgccatcg ccaagaacgt
      421 cgcgcgcgaa agtcttcgca cgcgcccgcg cgaggagcgc aaggtcgaga tcgacgaccc
      481 gctggtcgcc gagttgaagg actcgcgccg ctcgcccgcc ggggcgctcc tcgacaagga
      541 gctgaacgag gtcgtccgca aggcgctcga acggctggac gaggacaagc gggccgtctt
      601 cactttgaaa gttttccagc agcggagcta cgaggagatc gtggaaataa cgggtttcag
      661 tttgggcaag gtcaagaccg acctccaccg cgcgcgcgcc gagatgcggc gccgcatcgg
      721 gccttatctg gggggtgagc agtgatgaaa tgcgtggact gcctgccgct gctcgaagag
      781 ttcttcgacg gcgaggttga cgacaagacc aatggccgga tgcgcgacca cctggcggtg
      841 tgcgccgact gcgccgaagc cttcgacgcg ctgcgcgccg agcaggagat gttcctgcgc
      901 tacgaccgcg agctggaaat cagcccggcg ctctggcaag gcgtgcgcgc cgccatcgcc
      961 gacgaaaggc cgcgccccga gcgccgcccc tttctcctgc gcctgcgcga acggttggcc
     1021 gccgccgccg ccctcttcgc cctgcgaccc gcgctcgcct cctcgctcgc gctcatggcc
     1081 gtcggcatca ccgccgggct gctctggcaa gcggcctctc aacgcccgac gggcgtggcc
     1141 ccgacagaca tagccgacag caacggcggc gcgcgccccg cgccttcgac cgaccagacg
     1201 gcaataactt cgaccgcgcc gcaggttgct caaatcaacg acggtgagac gcccgacggc
     1261 gacgacgagc cgggcgaggt cgcgccggtg aagctcgacg gcgagcacgt gaagccggag
     1321 acggcggcgc gcgtctcctc gaagagtgcg cgcgtccccg ccgcgcccga cgccggtgac
     1381 ggcatcgtca ggttcctcga cgcagaccat gccatcacgg ctgacgactc gctgccatcg
     1441 gtggcctcgg cctcgcaggg cggcgacgat ctggcggacg ccgccgcgct gctcgacccc
     1501 gaagacgagg ccgtcgcccg ccacgtcgag cgcgcgcagg tgctcctgcg ctccttcaag
     1561 aacacgcgcg ccgccgaggg cgagggcgac ctcgcctacg agagggagct gtcgcgcaag
     1621 ctcttggaag agaacatcac gctccagctc gacgccgacg ccgacgggaa caagaaggcg
     1681 aagaaggtct tgagccagtt ggagccgtac ctgaccgaca tctcgaacct gcgcgaccgc
     1741 cccgagcgcg aacagctccg ctcgatcaag gagcgcatgc agaagcagga gatcatcgcc
     1801 gccctccacg tctacgacga ctagggccgc agccttttgg acggagccga gcacacccgc
     1861 tgtaatttaa aattgaggaa gaaagaatca tgttcatgcc aaccaccccc cgcactcatc
     1921 cgaaatcggt cgcgcgaatc gccgcgggcc tcgtgctcgg cgccacgctg ctcgccgggg
     1981 gcgccgccgg cgtccgcggc cagcagaaat tcacgcccga gcgcgagatg ctgaaccggc
     2041 tcgtccagtc gatgcagaac gacgcggcgg cgaaggcttt cgtccagggc cgcgaccaga
     2101 tcaacgaagg caagtgggcc accgccgcca cgaccttcgg caagtacgtc gccgactacc
     2161 ccgcggacaa gaacgtggac gccgcgctct actggctcgc ctacgcgcag gagaagcaga
     2221 agaagtacgg cgaggccgac gccgtgctcg ccaagctcat caaagagcac acgaagtccg
     2281 cttggaagaa cgacgccgag aagctgcgcc tgcttgtccg ctcgaagctc ggcaccctct
     2341 actacacggg caacaacccc gacagcctgc tcgacccgca gcaggcgcag aggccggacg
     2401 ccgactgcga gctgaaggtc gtcgcgctcc aggcgctctg tcaggccgac cgcggcgcgc
     2461 gctgctcgac ccacgtcaac gacgcgctcc gctcgaacac cacctgcctc gtcctgaagg
     2521 aggcggccat cacctacctc ggcaggtacg gcgggcagga cgcgctcccg gcgctcatcc
     2581 agatggcgcg caccgagccg aacgagaagc tgcgcatgcg ggccatcgcc gcgctcgggc
     2641 gcaccggcga cgagcgcgcg ctcgacgtgc tgcgcgagct ggcgatgagt cccgtctacg
     2701 agaacgaaag cccgaccgac tcggcgatcc acgcgctcgc cggcttcgag aacgcgcgcg
     2761 ccgccgccat cctcgccgac gtcatcatca acggcaagaa catcgaggcg cgcacgcacg
     2821 gcgcccaact gctcagccag cgccgcggcg acgacgtggt cgatcagctc ttccgcatct
     2881 acgacgccgc gagcgagatc gaggtcaaga agtacgtgct cgccggactc ggcaaccgca
     2941 aagacccgcg cgccatcgcc aagctcatcg aggtcgcgcg caacgacccg aacgaagcgc
     3001 tgcgcaagca ggccatccac tcgatcccga accgcaacga cgagcaggac ctcgacgtgc
     3061 tgatcccgct ctacgactcg gagcgcaacg ccgagctgaa ggagcacatc ctcgacgcca
     3121 tcggccacta ccagaaccag cgcgcctatc agaagctcat gcaggtcgtc cgcagcaacg
     3181 aatcgctgga gcggcgcaag aaggccatcc agatgctcag ccggagcaaa gaccccgcgg
     3241 tgctgaagtt cctcgaagac atgctcaagt agccgaagcg acgcttgcga ccaaaggagt
     3301 ctggccgcgc acttgtcagg aggcggccag actttgagcc ccccggactc aaggacaacc
     3361 gactctctcg ctcgaaccct tccacgcgaa cggggtgggg agagtgttcg cgtcggacag
     3421 gcgtctcggc gcccgtccga cgccggaagg agtttttcga tgctcgccac gttagcgttc
     3481 ttcctgctca tgctcacgcc gcccgccgcc gccccggcct cgccgcagga tcagaggttc
     3541 gtgcccgtcg agggcgccac cttgcaggca cggcaggagg ccgccgccag ccgcgccgcc
     3601 gcggcttcgc cgcagacgcc ctactggacg gcctactcgt tcgacgtgcg gcccggcgtc
     3661 gcgaccgact tcgagtgggt cggcgacgac ggcacgcaca tcatcctcga tggctcgtcc
     3721 gccttcgcct tcgaccccaa gcttgagacg cgcaacctcg gcgtcttcgt cctgcgctcg
     3781 aacgaagggg gcggcgccgt caagcgcgtc gaggtctaca acctcgcgcg cggccgcgag
     3841 tacagcggct accccgtcta ctgggcgggc cgcgcctcca acgacgagag cctgaacttc
     3901 ctgcgcggcc tcgccgagac ggcggcgacg cgcgcggacg acgtggccgc cgccgcgacg
     3961 cgcgcgctgg cgctgcacga cgaccgccgc gtcccctcgc tgctcgaagc ctttgcgcgc
     4021 acctcgacct cgacgcgcgt ccgcgcgcag gccgtccgct ggctcggcca caccaccgcg
     4081 gacaccgctc ctgcgccgcc gctcctttgg gagctggcgc gcagcgaccg cgagttcgtc
     4141 gaagtccgcc gcgcggcggt cggcgccctc ggccagcgca aggacgccga aagtctaggc
     4201 gtgctgcaaa gcctctacga ctcgctcgcg cagcgcgagc tgaagcggag cgtgctctcg
     4261 gccatcgggc gcaacgagaa ccgcagcgcg gcggccggct tcatcgtccg cgtcgccgag
     4321 cgcgagaccg acgttgaact caggaagcgc gccgtcgcgc agctcggcga gatcgcgggc
     4381 gagcaggcgc tcggcgcgct cgcccaaagg gccgcgcaga cggacgccga caccgaattg
     4441 cagaagcagg ccgtcaacgc catcgcccgc cgccccgccg ccgagtccgt cccgcttctc
     4501 atcaagatcg cccgcaccca ccagaagccc gaaatccgca agcaagcctt cctcctcctc
     4561 ggcaggacga acgaccccac ggccatcggc ttcctccgcg aagtcctgac gaagtaggca
     4621 gtaggcagta ggcggtaggc agttaaaacg gaagtccgag gtcaggagtc ggaagaagga
     4681 agaggcttga gattgaacgt ctcaagcctc ttcactttcc tgcccgtcga acgaagaggc
     4741 ttatagcggg gttcattcgt atgcgacttc cgccaaatgc cccatcttac gctcgtttct
     4801 agcgaacacg gcggcaaact gccagcatca ggtcgcatcc gaatgaccac cgctgtaaca
     4861 ctttaccccc ccaagcctct ccgcccttca ttactttgac tgcctgctgc ctgctgcccg
     4921 ccgcccgctt tcatgtaaac tggcgccgca tacacgacac gcgaggaggg ggatgtggat
     4981 ggccgttctt gagatcgtcg agttcccggc gaaggtgttg aggggggcgg gcgcgcccgt
     5041 ggagaagttc gacgaggagc tggggaaact cgtcgcggac atgttcgaga cgatgtacgc
     5101 ggcggagggc gtcggcctcg cggcgcagca ggtcggcgtg tccctgcgcc tcttcgtgat
     5161 ggactgcgag gggatcaagc tcgtcgccgc gaaccccgaa atcgtctccg ccgaaggcga
     5221 gcaggacggc gaagagggct gcctctcggt cggcaaaatc cacgcccccc tgcggcgcgc
     5281 caatcgggta accttacgcg cgcaggacga gcacggcgac ctttacgaac gcgaggcgac
     5341 cggcctcgcc gcccgttgct tcctccacga gaccgaccac aacgacggca agctcttcat
     5401 cgaccgcctc accccgctca aacgcgagat ggtcaagaag cgcttccaga agatgaagaa
     5461 gtggcggtga gaagaagtag gcggtaggca gtaggcggta ggcagttaag aaaagaaggg
     5521 cgaagatgct tgagcacgtt atctctcaag caccttcgcc ctctactttt tttaactgcc
     5581 cgctgcccgc tgcccgctgc ttgcttttct taactgccta ctgcctactg cttgacgtgc
     5641 tgaatcagga gcagcccgat cgagacgagc gcgagcacgt acatgatggg gtggacttcg
     5701 cgcgcgcggc ccgcgagcag gtagaggctg gcgtgggtga tgaagcccca gaggatgccc
     5761 tgcgtgatgg agaaggtgag ggggatgagg atgatggtga ggaaggcggg gagcgcgtct
     5821 tcgagccggt cgaaggcgag gcccgcgacg ctgcggaaca tcgagacgcc gaccatcagg
     5881 agcacggcgg cggtcgcgta aggcggcacc atcccggcga gcggcgcgat gaagaagcag
     5941 ggcaggaagc agagcgccgt gaagaccgag gtcagcccgg tgcgcccgcc catctcgatg
     6001 cccgccgtcg attcgatgta ggcggtgccc gacgaagtgc ccgcgagtcc cgcaccgagc
     6061 gtcgcccacg aatcgacgat cagcccctcg cgcaggcggc gcgggtggcc ctcttcgtcg
     6121 agcatcccgg cggcctgcga gacgccgatg aaggtcgaga tcgagtcgaa cagatccgtg
     6181 aacatgacgg cgatgatcgc cgggagcagc gagagccgga gcgcgccgag gaggtcgagc
     6241 ttgaggaaga cgctgtagaa gtccggcgcg ctgaagagct gcggcggcgc ctgcaccttg
     6301 cccaacgccc acgcggcgag cgtgacgacg aagatgcccg cgaggtaggc cagagggctg
     6361 cgccacttca gcagcagcgc gatgacggcc agccccagga gcatcagcag cgcacgatgg
     6421 tcgaggtcgc cgaggcggac gagcgtgacc gggtgcgcgg cgacgagtcc cgcgttttgc
     6481 agtccgatga aggtca
//
LOCUS       DBNGOH010000086         2495 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1479110, whole genome shotgun sequence.
ACCESSION   DBNGOH010000086 DBNGOH010000000
VERSION     DBNGOH010000086.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2495)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2495
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1479110"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..607
                     /locus_tag="AC159P_01635"
     CDS             <1..607
                     /locus_tag="AC159P_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016944736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3694181.1"
                     /translation="WAFVIAKFMTDSIWRWYLYLLPLFFSQAFGLSIFKFGAAFVVIY
                     VLADVGSIAGGWLSSKLIASGRSVNFARKVALLVCALCVVPVVFVTQMSNVWMAVLLV
                     GLAAAAHQGWSANLFTTASDMFPRQAVGSVVGLGGMAGALGAMLILKVTGWILGDTSK
                     ADAGTYLPLFIIAGSAYLLALLIIHVLVPRLEPARIEGEAV"
     gene            824..1831
                     /locus_tag="AC159P_01640"
     CDS             824..1831
                     /locus_tag="AC159P_01640"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: protein motif:HMM:NF014477.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HIR3694182.1"
                     /translation="MQTIKRREFLNPAAGRRAPSQSYWVHVSRAAMACRFEVTLPARE
                     RAGVEAARAALDEVDRIEEQLTVFRETSEVSHVNRRAAFEAVRVEASLFGLLELSEGL
                     RRETAGAFDVRSGPLSRCWGFMRREGRVPDAREIEDARSRVGHGALILDAEARTVRFA
                     RAGVEINFGSIGKGYALDCVAAGVGSRVRSALLSAGFSSMRAVGGGDAGQRGWVVGVR
                     DPRRKDRRMAVLRLRDCAMSTSGSEEQFFEHGGRRYGHIIDPRTGYPAEGVSGVTVVA
                     ESAARSDALATAFYVGGRALAESYCAEHAGVLAVMLESGAERPVVIGGNEKCEVELID
                     E"
     gene            1824..2369
                     /locus_tag="AC159P_01645"
     CDS             1824..2369
                     /locus_tag="AC159P_01645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX subfamily"
                     /protein_id="HIR3694183.1"
                     /translation="MSNGTPYSLSSVQQFALVLLRTLIGWHFLYEGYYKLMLPGWGPD
                     GKPLGAWTSAGYLRGASGPLAGLFKRMVDAGWTPWIDGAVKFTLIAIGLSLILGLFTR
                     VGAVAALALLALFYVLAIPWAGVPQPGNEGTYLIVNKTLIEAAAVCVLLAFNTGAIAG
                     LDLLLSRRRRRVASPVEAKAV"
BASE COUNT          349 a          761 c          928 g          457 t
ORIGIN      
        1 atgggccttc gtcatcgcca agttcatgac cgactcgatc tggcgctggt atctgtacct
       61 gctgccgctc ttcttcagtc aggcgttcgg gctgagcatc ttcaaattcg gcgcggcctt
      121 cgtcgtcatc tacgtgctgg cggacgtagg ctccatcgcg ggcggttggc tctcttcgaa
      181 actgatcgcc tccgggcgct cggtcaactt cgcgcgcaaa gtggcgctgc tggtctgcgc
      241 gctctgcgtg gtgccggtcg tcttcgtgac gcagatgtcg aacgtgtgga tggcggtgct
      301 gctcgtcggg cttgcggcgg cggcgcacca gggctggtcg gccaatctgt tcacgaccgc
      361 gtcggacatg ttcccgcggc aggccgtagg ctcggtggtc ggtttgggcg ggatggcggg
      421 cgcgctcggc gcgatgctga ttctgaaggt gacgggctgg atcctcggcg acacgagcaa
      481 ggccgacgcc ggcacctacc tgccgctctt catcatcgcc ggctcggcct atctcttggc
      541 tttgctcatc atccacgtcc tcgtgccgcg gctcgaaccg gcgcgcatcg agggcgaggc
      601 cgtctgacgc ggagagtttc gacgatccgg acggtctttc aagatcgagt gtcggccagc
      661 aacagcgaca cgccgccgcg gacgcgggcg acacgggggt tgcacggagg gtcgaacgat
      721 tcgggattcg agatttcgaa ttcgaagttc tccgtgccgc cttcgcgtcc tccgcttccg
      781 cggcggtgat aaagacggac tctgagtgcg ccgacctttt cgcatgcaga cgatcaagag
      841 acgagagttc ctgaacccgg cggcgggccg ccgcgcgccg tcccaaagct attgggtgca
      901 cgtgagccgc gcggcgatgg cgtgccggtt cgaggtgacg ctgcccgcgc gcgagcgcgc
      961 gggcgtcgag gccgcgcgcg cggcgctcga cgaggttgac cgcatcgaag agcagttgac
     1021 cgtcttccgc gagacgagcg aggtcagcca cgtcaaccgc cgcgcggcgt tcgaggcggt
     1081 gcgcgtcgag gcttcgctct tcggtctgtt ggaactcagc gaggggctgc ggcgcgagac
     1141 cgcgggcgcg ttcgacgtga gatcggggcc gctcagtcgc tgctgggggt tcatgcgcag
     1201 ggaagggcgc gtgcccgacg cgcgcgagat cgaggacgcg aggtcgcgtg tcgggcacgg
     1261 agctttgatt ctcgacgccg aggcgcggac ggttcgcttc gcgcgcgcgg gcgtcgagat
     1321 caacttcggg agcatcggca agggctacgc gctcgactgc gtcgcggcgg gcgtcggcag
     1381 ccgggtgcgt tcggcgctcc tgagcgcggg cttcagcagc atgcgcgcgg tcggcggcgg
     1441 cgacgcgggc cagaggggtt gggtcgtcgg cgtgcgcgac ccgcgacgca aagacaggcg
     1501 gatggccgtg ctccgtctgc gcgactgcgc gatgtccacg agcgggagcg aggagcagtt
     1561 cttcgagcac gggggccgcc gctacggtca catcatcgac ccgcggacgg gctaccccgc
     1621 ggagggcgtg tcgggcgtga cggtggtcgc ggaatcggcg gcccgctcgg acgcgctcgc
     1681 caccgcgttc tacgtcggcg gtcgcgcgct ggccgaaagc tattgcgcgg agcacgcggg
     1741 cgtgttggcc gtcatgctcg aaagcggggc ggagcgcccg gtcgttatcg gcggcaacga
     1801 gaaatgcgag gtggagttga tcgatgagta acgggacgcc gtacagtctc tcttcggtgc
     1861 agcagttcgc gctcgtgttg ttgcggacgc tgatcggttg gcactttctc tacgagggct
     1921 actacaagct gatgctgccc ggctgggggc cggacggcaa gccgctcggc gcgtggactt
     1981 ccgcaggtta cttgcggggc gcgtcggggc cgctcgcggg tctgttcaag cggatggtgg
     2041 acgcgggctg gacgccgtgg atagacggcg cggtgaagtt cacgctcatc gccatcggcc
     2101 tctcgctcat cctcgggctg ttcacgcgcg tcggcgcggt ggcggcgctc gcgctgctcg
     2161 cgctcttcta cgtgctggcc ataccgtggg cgggcgtgcc gcagccgggc aacgagggca
     2221 cgtacctgat cgtcaacaag accctcatcg aagcggcggc ggtctgcgtc ctgctcgcct
     2281 tcaacacggg cgcgatagcg ggtctggatt tgttgttatc gagacgaagg cggcgcgtgg
     2341 cgagtcccgt cgaggcgaaa gccgtgtgat gttttccgcc gcgtagcggc gaggtgaatt
     2401 tagccgtgcc cttcagggca cggtaaagga tgcggaaaat ttttccgtcg cgtcagcgac
     2461 gggtgagcct tgattcaagc gtcgctgacg cgacg
//
LOCUS       DBNGOH010000087         1629 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4779030, whole genome shotgun sequence.
ACCESSION   DBNGOH010000087 DBNGOH010000000
VERSION     DBNGOH010000087.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1629)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1629)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1629
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4779030"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1584)
                     /locus_tag="AC159P_01650"
     CDS             complement(<1..1584)
                     /locus_tag="AC159P_01650"
                     /inference="COORDINATES: protein motif:HMM:NF013100.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HIR3694184.1"
                     /translation="MTRKFPSPFILALALVFTFSLANTAHAAGPKKKKAPAKATAAKT
                     AKGKKGAAAKNERASSRRETARQKNARAGRDKRSRAEERASARNARDRRSSRSERAEK
                     NRRTDRRDDARKDNRRMSRRERLLEARREAERRRREAAERARQAAIARALYLARIRAM
                     DQGFRDETVANILKDDTTGEDPEVRRVAVQALGERAGTVVVMEPKSGRVLTVVNQDGA
                     LRKGFKPCSTIKLVTGLAGLNEKVIDPVQTVNIGEGTFRLDLTDSLAFSNNGYFQKVG
                     GEVGFEKMMEYARKMGLGQPTGINHPFESPGKLPVFKQGWSTNRMSSHGDDIEVTAVQ
                     LANMASAIANGGKLLVPHLPRTPQENVNFKREVKREIDVPQEHVQRLVPGMIGAVNYG
                     TAKRAQDPLQTVAGKTGSCIEQSSQRPWVGLFTSYAPVHDPKLAVAVILRSSGARGKW
                     ASAVAGEIYRNLHHRFGIRPGSAPALADEKLAPSRPKIDARAAALLSDEEKEDEANEA
                     TAADAYVVSESNGAPSRTTD"
BASE COUNT          202 a          532 c          592 g          303 t
ORIGIN      
        1 gtcggtcgtc cggctcggcg cgccgttgga ttcgctcacg acgtaggcgt ccgccgccgt
       61 cgcctcgttc gcctcgtcct ccttctcctc gtcgctcaag agggcggcgg cgcgggcgtc
      121 gatcttcggg cgcgagggcg cgagcttctc gtcggcgagc gcgggcgcgc tgcccggtcg
      181 gatgccgaag cggtgatgga ggttgcggta gatttcaccc gcgaccgccg aggcccactt
      241 gccgcgcgcg ccgctgctgc ggaggatgac ggcgacggcg agcttcgggt cgtggacggg
      301 cgcataggag gtgaagaggc cgacccacgg gcgctgcgaa ctctgctcga tgcacgagcc
      361 ggtcttgccc gcgaccgtct gcaaggggtc ttgcgcgcgc ttcgcggtgc cgtagttgac
      421 ggcgccgatc atgccgggaa cgagccgctg cacgtgctcc tgcggcacgt cgatctcgcg
      481 cttgacctcg cgcttgaagt tgacgttctc ctgcggcgtg cgcggcaggt gcgggacgag
      541 gagcttgccg ccgttggcga tggccgaggc catgttggcg agctggacgg cggtgacttc
      601 gatgtcgtcg ccgtgcgagg acatgcggtt cgtagaccag ccctgcttga agacggggag
      661 tttgcccggc gactcgaacg ggtggttgat gccggtcggc tggccgaggc ccatcttgcg
      721 cgcgtactcc atcatcttct cgaagccgac ctcgccgccg accttctgaa aatagccgtt
      781 gttcgagaag gcgagcgagt ccgtcaggtc gaggcggaag gtgccctcgc cgatgttcac
      841 ggtctggact ggatcgatca ccttctcgtt cagaccggcg aggcccgtca cgagcttgat
      901 ggtcgagcac ggcttgaagc ccttgcggag cgccccgtcc tggttgacca ccgtcagcac
      961 gcgccccgac ttcggctcca tgacgaccac ggtgcccgcg cgttcgccga gcgcctgcac
     1021 cgcgacgcgg cgcacctcag ggtcttcgcc cgtcgtgtcg tccttcagga tgttggcgac
     1081 cgtctcgtcg cggaacccct gatccatcgc gcggatgcgc gcgaggtaga gggcgcgtgc
     1141 gatggcggcc tggcgggcgc gctcggcggc ctcgcggcgg cggcgctcgg cctcgcggcg
     1201 ggcttcgagc aaacgctcgc ggcgcgacat ccgccggttg tctttacgcg cgtcgtcgcg
     1261 gcggtcggtg cgccgattct tctcggcgcg ctcgctgcgc gacgagcggc ggtcgcgggc
     1321 gttgcgcgcg gacgcacgct cttcggcgcg gctgcgcttg tccctcccgg ctctggcgtt
     1381 cttctgccgc gccgtctccc tgcgcgaaga ggcgcgctcg ttcttggcgg cggcgccttt
     1441 cttacccttg gcggtcttcg cggctgtggc tttcgcgggc gccttcttct tcttcgggcc
     1501 ggcggcgtgg gctgtgttgg cgagtgaaaa agtgaagacg agggcaagcg ccaggatgaa
     1561 gggggacgga aacttacggg tcataacgga gtcctctgct tgaaaaaaca aagccgagtg
     1621 cggacagcc
//
LOCUS       DBNGOH010000088         2681 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_196360, whole genome shotgun sequence.
ACCESSION   DBNGOH010000088 DBNGOH010000000
VERSION     DBNGOH010000088.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2681)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2681)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2681
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_196360"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(29..610)
                     /locus_tag="AC159P_01655"
     CDS             complement(29..610)
                     /locus_tag="AC159P_01655"
                     /inference="COORDINATES: protein motif:HMM:NF028235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR3694185.1"
                     /translation="MARHKEFDRDEALHKAMEVFWSRGYEAASVGALVRHMGINRQSL
                     YDTFGDKQALYLQALDRYREVEGRKMFELLERPGSVKKALRQLFEGVLDGSLCGGERR
                     GCFVGNAMSERAGRCPETAARACGQLAAAEEALYRALLRGKRAGEIEGGRDLRAVARF
                     LYSSLQGLQLMAKATQDRKTLHDVVKVTLSVLD"
     gene            767..2614
                     /locus_tag="AC159P_01660"
     CDS             767..2614
                     /locus_tag="AC159P_01660"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3694186.1"
                     /translation="MAERGRKKIRWSSAWRDARELVWAHRRRLLLGAGLMLVNQAMGL
                     VLPASTKYLIDEVIGKGRTEMLGRIALAAGAATLVQAVTSFSLSQVLGVAAQRAITEM
                     RKRVQAQVARLPVGYFDSTQTGQLISRIMNDAEGIRNLVGTGLVQLAGSFITAIVALC
                     VLFWLNWRLTAITILVLGLFGGALAYAFTRLRPLFRERGEIMAALTGRLAESLGGIRI
                     VKAYTAERREALTFARGAHKLFRNIARSMTGVSATTAFSSVVVGAVGVVMILVGGRSV
                     VAGAMTLGDLFMYIIFTGLLAMPLIQFAAIGTQITEAFAGLDRIREVMNMATEDEEDA
                     VRAPLADVRGEVEFEDVWFAYDEDVHVLKGVSFKAPAGSTTALVGSSGSGKSTLTSLV
                     MNFNQPLSGTVRIDGRDLQTLRLRDFRSHLGVVLQDNFLFDGTIAENIGFSKPHATRA
                     EIEAVSRVAHCAEFIDKFEKGYDTVVGERGVKLSGGQRQRIAIARAILADPRLLILDE
                     ATSSLDSESEALIQDGLRQLRRGRTTFVIAHRLSTIRSADQILVLEEGRIVERGTHDE
                     LLAAGGRYRQLYDKQYKFERDQFINPGEDFTPEPARPLSGGAPPRLSNL"
BASE COUNT          444 a          913 c          885 g          439 t
ORIGIN      
        1 cgcttgttcc agaacatccc aaaaaaattc agtcgagcac cgagagcgtc accttcacta
       61 catcgtgcag ggtctttcgg tcctgcgtcg ccttcgccat cagttgcagc ccctgcaagc
      121 tgctgtacag gaaccgcgcg acggcgcgca ggtcgcgccc cccctcgatc tcgcccgccc
      181 tcttcccgcg caggagcgcg cggtataaag cctcctccgc ggcggccagt tggccgcacg
      241 cacgggccgc ggtctccggg cagcgcccgg cccgctccga catcgcgttg ccgacgaagc
      301 agccgcgccg ctcgccgccg cagagcgacc cgtccaggac gccctcgaac aactgccgca
      361 gcgccttctt cacggagccg ggccgttcca acagctcgaa catcttccgg ccctccacct
      421 cgcggtagcg gtcaagcgcc tgcaagtaga gcgcctgctt gtcgccgaag gtgtcgtaga
      481 ggctctgacg attgatgccc atgtgcctga ccagcgcgcc cacggacgcg gcctcgtagc
      541 cgcgcgacca gaagacctcc atcgccttgt ggagggcctc gtcccggtcg aactctttgt
      601 gccgcgccat ccgactttct ttacctctgg agagggttta tactctttct ggaacgatta
      661 gtcaagaatc aatcctgccg ccggacagcg gcgggttttt gactccttcg cgggaacaac
      721 ctttaatgca ccgatatgaa tgaaacgata agccgggcgg tgaggcatgg ctgagcgtgg
      781 acggaagaag attcgctggt cgtcggcgtg gcgcgacgcg cgcgagctgg tgtgggcgca
      841 caggcgtcgg ctgctgctcg gcgcggggct gatgctcgtc aaccaggcga tggggctggt
      901 gctgcccgcc tcgaccaagt acctgatcga cgaggtgatc ggcaaagggc gcacggagat
      961 gctcgggcgg atcgcgctgg cggcgggtgc ggcgacgctc gtgcaggccg tcacctcctt
     1021 cagcctgtcg caggtgttgg gggtggcagc gcagcgcgcc atcacggaga tgcgcaagcg
     1081 ggtgcaggcg caggtcgcgc ggctgccggt cggctatttc gactcgacgc agaccggcca
     1141 gctcatctcg cgcatcatga acgacgccga aggcatccgg aacctcgtcg gcacggggct
     1201 ggtgcagctc gccggcagct tcatcacggc catcgtcgcg ctctgcgtcc tgttctggct
     1261 caactggcgt ctgacggcga tcacgatcct cgtgctcggt ctgttcggcg gcgcgctggc
     1321 ctacgccttc acgcggctgc gtccgctgtt ccgcgagcgc ggcgagatca tggcggcgct
     1381 gacggggcgg ctggccgaat cgctcggcgg catccgcatc gtcaaagcct acacggcgga
     1441 gcggcgcgag gcgctgacgt tcgcgcgcgg ggcgcacaag ctcttccgca acatcgcgcg
     1501 ctcgatgacc ggcgtgtcgg cgacgaccgc cttctcgtcg gtcgtcgtcg gcgcggtcgg
     1561 cgtggtgatg attctggtcg gcgggcgttc ggtcgtggcg ggcgcgatga cgctcggcga
     1621 cctcttcatg tacatcatct tcacggggct gctggcgatg ccgctcatcc agttcgcggc
     1681 catcgggacg cagatcaccg aagccttcgc gggcctcgac cgcatccgcg aggtgatgaa
     1741 catggcaacc gaggacgagg aggatgccgt gcgcgcgccg ctcgcggacg tgcgcggcga
     1801 ggtcgagttc gaggacgtgt ggttcgcgta cgacgaagac gtccacgtcc tcaaaggggt
     1861 ctccttcaaa gcccccgccg gctcgacgac ggcgctcgtc ggctccagcg gctcgggcaa
     1921 gagcacgctc acaagcctcg tcatgaactt caaccagccc ctctccggca cggtcaggat
     1981 cgacgggcgc gacttgcaga cgctccggct gcgcgacttc cgctcgcacc tcggcgtcgt
     2041 cttgcaggac aacttcctct tcgacggcac catcgccgaa aacatcggct tctccaaacc
     2101 gcacgccacg cgcgcagaga tcgaggcggt gagccgcgtc gcgcactgtg cggagttcat
     2161 cgacaagttc gagaaggggt atgacacggt ggtcggcgag cgcggggtga agctctccgg
     2221 cgggcagcgc cagcgcatcg ccatcgcgcg cgccatcctc gcggacccgc ggctgctcat
     2281 cctcgacgag gcgacttcca gcctcgacag cgagagcgag gcgctgatac aggacggcct
     2341 gcgtcaactg aggcgcgggc gcacgacctt cgtcatcgcg cacaggctct ccaccatccg
     2401 cagcgccgac cagatcctcg tcctcgaaga ggggcgcatc gtcgagcgcg ggacgcacga
     2461 cgagctgctg gccgcgggcg gccgctaccg ccagctctac gacaagcagt acaagttcga
     2521 gcgcgaccag ttcatcaacc ccggcgaaga tttcacaccc gagccggcac gccccttgag
     2581 cggaggcgcg ccgccgcgcc tctcgaacct gtaagtcgag tatagtgcgc gctcacttca
     2641 aagcagaggg cacggagaag cgcggagggc gcggagaaag a
//
LOCUS       DBNGOH010000089         3150 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4779167, whole genome shotgun sequence.
ACCESSION   DBNGOH010000089 DBNGOH010000000
VERSION     DBNGOH010000089.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3150)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3150)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3150
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4779167"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            302..880
                     /locus_tag="AC159P_01665"
     CDS             302..880
                     /locus_tag="AC159P_01665"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3694187.1"
                     /translation="MGDTCAPNCSAMKVLDIGCGKHKTPGAVGLDSNPRTGADVIHDL
                     DTVPYPFPDDEFDVVVGNQVIEHVEDVLAVVAELHRITRPGGLIRLDTPHFSDVASYT
                     DPTHKHHLTTESFAYVTGDRPDFDFYSHVRLRPRMVRVTMLKLWRALGFELLVNSCNR
                     YPALRFLRRFWEQYLCFVVRGKTIYFEFEVIK"
     gene            930..1469
                     /locus_tag="AC159P_01670"
     CDS             930..1469
                     /locus_tag="AC159P_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694188.1"
                     /translation="MSSKPKKAKGEGELPQPKRASNRPSKKDQIISLFLSGMGEVEDI
                     AIITGSRPSYVGSVLQEAGLVQGYFDLYTSTAHPQNVYSKFFAGKLGFKDEETARRSV
                     ELIDRLYRQFEFAGDRAGQHHALAMALTMFDRARWTNKGREAEIFQSWLVSRLGEAVV
                     TEGDDEAAGETEEAPDELS"
     gene            1656..3050
                     /locus_tag="AC159P_01675"
     CDS             1656..3050
                     /locus_tag="AC159P_01675"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HIR3694189.1"
                     /translation="MRGRNNIPFILALLVCLFSAAARADAVDDYVREQMRRRHVPGLA
                     LAVVRDGKLVKARGYGLASVELNVPVRVETVFEIGSVTKQITAAAVMLLVEEGKLGLD
                     DPVGKHLPGTPDAWSAVTVRHLLTHTSGLKNYTGLRGFELTERLKREDFIRLVAAHPL
                     AFAPGDAHAYGNTNYSLLGYVVEAVSGKPYWQFVAERIFKPLGMNATRDRDPHHVIPN
                     RAVGYEWEDGQLVGRDYDLTDVFAAGATVSTVLDLVKWDAALAGEKLLKKSSLEQVWT
                     PTRLGDGTTHPYGLGWYVENVRGHGRVRHNGQTAGFAASIARYRDARVTVIVLTNLGT
                     LGLAGRINQGVAKHYLPALSLKRLGDQTDPDPQTSARLRSALADLLAGRVEPAAFTRE
                     RGAALATSNAKEFWRDIASYGPLKSLAFVEREPSAKSRTLRYKAALGEHLLLVRFVLE
                     DDGRIAEVNVEEEE"
BASE COUNT          550 a         1159 c          947 g          494 t
ORIGIN      
        1 acgccgtgca caggagcgcg cgcgcctccc cgaaggcgaa cgcttcccgc cgcccgacgg
       61 tcgccgcccg ccgccgcccg acggtcgccg cccgcccccg ccgcaccagc gccccttccc
      121 ctgatgccgc gccggcccca ccgaatcaac tcggctcgta gtcgtctctg acgcgctctc
      181 tgcgtcatac tcggactccc gaccaactca acccgcggac tgacgcgccg acaccgcgtc
      241 ggcgctcgcg cttgtctgcc gcttcgcgcg gaggctacac tcgcccttta agctccgtcc
      301 aatcggcgac acctgcgcgc ccaactgttc cgcgatgaaa gttctcgaca tcggctgcgg
      361 caagcacaag acgccgggcg cggtcggcct cgacagcaac ccgcgcacgg gcgccgacgt
      421 gattcacgac ctcgacacgg tgccctaccc tttccccgac gacgagttcg acgtggtcgt
      481 cggcaatcag gttatcgaac acgtcgagga cgtgctcgcc gtcgtcgccg agctgcatcg
      541 catcaccagg ccgggcggcc tcatccgtct cgacacgccg cacttctcgg acgtggcctc
      601 ctacaccgac ccgacgcaca agcaccacct cacgaccgag tcgttcgcct acgtcaccgg
      661 cgaccgcccc gacttcgact tctacagcca cgtccggctc cggccccgca tggtgcgcgt
      721 cacgatgctc aagctctggc gcgcgctcgg cttcgagctg ctcgtcaaca gttgcaaccg
      781 ctaccccgcc ctgcgcttcc tgcgccgctt ctgggagcag tacctctgct tcgtcgtccg
      841 cgggaagacc atctatttcg agttcgaagt gataaagtag cgacgaacat tctcggacac
      901 agggaacgag gaaaggagag cttagagaca tgagcagtaa accgaagaag gcgaagggcg
      961 agggcgaact gccgcagccg aagcgcgcgt cgaaccgccc gagcaagaag gatcagatca
     1021 tctcgctctt cctgtcgggc atgggcgagg tcgaggacat cgccatcatc acggggtcgc
     1081 gcccctctta cgtcgggtct gtgttgcagg aggcgggcct cgtacagggc tacttcgacc
     1141 tctacacctc gacggcgcac ccgcagaacg tctactccaa attcttcgcc ggcaagctcg
     1201 gcttcaagga cgaagagacc gcgcgccgga gcgtcgagct gatcgaccgc ctctaccgcc
     1261 agttcgagtt cgccggcgac cgcgccggcc agcaccacgc gctcgccatg gcgctcacca
     1321 tgttcgaccg cgcccgctgg acgaacaagg gccgcgaggc agagattttc cagagctggc
     1381 tcgtctcgcg cctcggcgaa gccgtcgtca cggaaggcga cgacgaagcg gcgggcgaga
     1441 ccgaagaggc gccggacgag ctttcttgac agcgtccgtc agactgtagg ggcagggcaa
     1501 gtccgcggcc cgccgacgtt ggattgattc aacgttgaat gtttcgtcag cgcgggccgc
     1561 tgacaagccc tgctcctaca aaaatcacga cggttcgcat ccagccgcgg actcatcccc
     1621 aaccgacttt cgcttttgga gcgacaacaa gatcgatgcg cggtcgaaac aatattccct
     1681 tcatcctcgc gctgctcgtc tgtctattct cggcggcggc gcgcgctgac gcggttgacg
     1741 actacgtccg cgaacagatg cggcggcgcc acgtgccggg tctcgcgctg gcggtcgtgc
     1801 gcgacgggaa gctggtcaag gcgcgcggct acgggctggc gagcgtcgag ctgaacgtgc
     1861 ccgtccgcgt cgagaccgtc ttcgagatcg gctccgtcac gaagcagatc accgccgccg
     1921 ccgtcatgct cctggtcgaa gagggcaagc tcggcctcga cgaccccgtc ggcaagcacc
     1981 tgccgggcac gcccgacgcg tggagcgccg ttacggtgcg tcatctgctg acgcacacct
     2041 ccggcctcaa gaactacacg ggcctgcgcg gcttcgagct gaccgagcgc ctcaagcgcg
     2101 aggacttcat ccgcctcgtc gccgcgcacc cgctcgcctt cgcgcccggc gacgcgcacg
     2161 cctacggcaa caccaactac tcgctgctcg gctacgtcgt cgaggccgtc tccggcaagc
     2221 cttactggca gttcgtcgcc gaacgcatct tcaagccgct cggcatgaac gccacgcgcg
     2281 accgcgaccc gcaccacgtc atcccgaacc gcgccgtggg ctacgagtgg gaggacggcc
     2341 agctcgtcgg gcgcgactac gatttgaccg acgtgttcgc ggcgggcgcc accgtttcga
     2401 ccgtgctcga cctcgtcaag tgggacgcgg cgctcgcggg cgagaagctc ctgaagaaat
     2461 cgagcctcga acaggtctgg acgccgacgc gcctcggcga cggcacgacg cacccctacg
     2521 gcctcggctg gtacgtcgag aacgtgcgcg gccacgggcg cgtgcgccac aatggccaga
     2581 ccgcgggctt cgccgcctcc atcgcgcgct accgcgacgc gcgcgtcacc gtcatcgtgc
     2641 tcaccaacct cggcacgctc ggcctcgccg gccgcatcaa tcagggcgtc gccaaacact
     2701 acctccccgc gctctccctg aagcgcctcg gcgatcagac agaccccgac ccgcagacct
     2761 ccgcgcgcct gcgctcggcg ctcgcagacc tgctcgcggg gcgcgtcgag cctgcggcct
     2821 tcacgcgcga gcgcggcgcc gcgctcgcca cgtccaacgc gaaagagttc tggcgcgaca
     2881 tcgcctccta cggcccgctc aagtccctcg ccttcgtcga gcgcgagccg tcggccaagt
     2941 cgcgcacgct gcgctacaaa gccgcgctcg gcgaacacct cctgctcgtc aggttcgtcc
     3001 tcgaagacga cggcaggatc gccgaggtga acgtcgaaga agaagagtga acggattgtg
     3061 gattgtggat tgtggattgg cgtcctgctc atgcttgaag cctgattttg aaagttcaac
     3121 cctcgtgtcc gaacaatcca caatccacaa
//
LOCUS       DBNGOH010000090         8748 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3862878, whole genome shotgun sequence.
ACCESSION   DBNGOH010000090 DBNGOH010000000
VERSION     DBNGOH010000090.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8748)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8748)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8748
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3862878"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..513)
                     /locus_tag="AC159P_01680"
     CDS             complement(<1..513)
                     /locus_tag="AC159P_01680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694190.1"
                     /translation="MSKSPRPDGEGYAVEVSLLKFKPPTLALAAACLVTLVVLLGWLG
                     FGDVRQHAASQPLKNHAANQQPREAPAAIPNSATSQAAPHTSRAEAAPAPDRKSTDLA
                     FAVPEPSPAGFAETPAPANAQALAPSNAAPEAPQVVRNDAAAARVPAGGGYVGQAGSF
                     NTAAEANERVS"
     gene            1063..3576
                     /locus_tag="AC159P_01685"
     CDS             1063..3576
                     /locus_tag="AC159P_01685"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF012772.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HIR3694191.1"
                     /translation="MIILNSERAAKSLAWVSVALGACAIVLSVGGEDLWRPDARLAFL
                     TLFTLSVGATVLVKVPRSKAYVAASDVAVFVALLLYDASVAVPLAALSTLAAANRFDR
                     RTTSPATFAALAALATLAAVWCVNLVTASVAIAQPAAVLAVNLLALAAARALFGAVPA
                     LFIASGKEQRGGVGAYASASAWGFVVYLVAASLAGLVLTAAEWVSVDVSLAVFAALCV
                     AYHAYNVYAKGGGARPGEPVAGGRVALPSEGAESLRLAFDDAPTGMAILSARGELLKV
                     NRSFSRMLGRTDAELLLSSGIQDFVHADDLGPVLAGVKNVIKGGGSTLSTDVRHPLKD
                     GRIAWAHWNVGRFVAPYSDEVRLILQAQEVTERKLAEEKLLHDACHDLLTGLPNRALF
                     IDHVKLSISRARRREGSLFAVLFLDLDRFKLINDSLGHMVGDELLVVVARRLERCVRE
                     GDTVARVGGDEFMVLLDDLQDIGEAVQVAERIQNEVKVPFNVNSHEVFTTMSIGIAPC
                     SSEYDNPEDIMRDADTAMYRAKSLGKARHEVFDKTMHAFAVNLLQLETDLRKALDRDQ
                     FFIHFQPIVSLDTFHICGFEALVRWQHPERGSISPLDFIPVAEETGLIVAIGEWTLHE
                     SCRQMREWQREFPTQEPMFVSVNLSTKQFNQPNLIETVADILRETGLNPRALKLEITE
                     SAVMENIDVATDMLRQLRALGVQLSIDDFGTGYSSLSYLHRFPIDTLKIDRSFVTQMA
                     ENNENTEIVRTIVVLAQNLGMDVVAEGVETNDQLALLRQLGCEYGQGYYFSKPVSSAE
                     AGGIIADTRALQPVRAKGDMRRLPRLGGEPSTYTAAPAA"
     gene            complement(3747..4133)
                     /locus_tag="AC159P_01690"
     CDS             complement(3747..4133)
                     /locus_tag="AC159P_01690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694192.1"
                     /translation="MNTQSKKGQDGREKVSLPCSFGMSDDAPRKGTVTSLSVKRCFVK
                     TKAWAKDGMELHVKLWLPEERWLPLRATVLYCMEKVGFELLFDNPTPGETEALRALVG
                     AAQAGRLRAGDGLEIAEDEPVALEVE"
     gene            4429..5349
                     /locus_tag="AC159P_01695"
     CDS             4429..5349
                     /locus_tag="AC159P_01695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HIR3694193.1"
                     /translation="MNCRMKCRIFVAAVLIIAAAIIGQRMSGRQDTSGNSGAGREETR
                     RTYALDAGAEVEVYGINGSVEVNTAETNAAEVSIVRTAGSAEDLEHSRVVVEHTPSRL
                     VVRGEGHGKGGFWRWFRGGGHVRHQIVLTVPRGVELETKGINGPVRIGEVDGAVSVNG
                     VNGRVEVAQAAGRSEVQGVNGAVTITVARLGAEGMEVQGVNGRVEIQLKEVLNADINV
                     RRLNGSFRLDAPNVTMQEREDRSNMRARLGTGGPAVNITGVNGSVRFESTATAVNAIT
                     PVAPIAPVVVAVPDAPDAPHAPHPPADDHR"
     gene            complement(5587..5964)
                     /locus_tag="AC159P_01700"
     CDS             complement(5587..5964)
                     /locus_tag="AC159P_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015236503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR3694194.1"
                     /translation="MTERRHRVTLDEAMSRLPGPGGERFAEVLEHGTLVVEVYAPRGV
                     DAQQPHTRDELYVVMSGSGEFVNGDTREGFGPGDVLFVPAGVRHRFENFTADLAVWVV
                     FYGPEGGEAGAHESGFQMGVQRA"
     gene            complement(6050..7246)
                     /locus_tag="AC159P_01705"
     CDS             complement(6050..7246)
                     /locus_tag="AC159P_01705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694195.1"
                     /translation="MAVVVVVCVSSVAATAQGVVREIAPPTGEGSGQSFLSVGRDGRV
                     YLSWIDRLAEKRVALRFAVREGAGWSAPRTVAEGENWFVNWADFPSVVGLADGSLAAH
                     WLVRNGPGTYAFDVRLARSFDGGRTWGEAVTPHRDGTQTEHGFVSMFSAAGDRLGAVW
                     LDGREMKPAGGAGGHAHGEMTLRHATLARDGALADEEVLDARVCECCQTSAALTSEGV
                     VVVYRDRVEGETRDISIVRRARGGGWTKPRTVHADGWKIDACPVNGPSVAASGRRVAV
                     AWYTMAGGRPSVRLAFSGDAGASFGGASEVGDGDPVGRVDLLLLEDGSALVGWVEKTK
                     AGAELRARRVWPSGRRGAAVTVAPAGAVRPSSFPQMVRARDKIIFSWTGTARVLTAEM
                     PLDASR"
     gene            7605..8618
                     /locus_tag="AC159P_01710"
     CDS             7605..8618
                     /locus_tag="AC159P_01710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694196.1"
                     /translation="MLSRKRPVVVSGWMLLLLALVCAPAASAQTGRQQPTPTPQNPPT
                     ITLTADNEVVTLCPDNVSVANPRVRLRAVGNSPDGNPLRYRWTTSGGRIEGDGTDVVW
                     DLSNAQPGVYSATVTVESGPAGDPLCTAFTSTRVVVRNCPPPRPFCPNVSMYCPDVVQ
                     TGAPVTFTANTSGGTPGVTPVYNWKVSAGTITSGQGTPSITVDTAGLGGQPITASLEV
                     EGYNLVCRATCQSSVPAPVVARRADEFGEVNRDDEKARLDLFAIEMQNEPGARGYILG
                     YAGRKARPGEGQRRADRARDYLVNTRGIESTRVVVVDGGFAETGRTELWVVPPGATPP
                     RPR"
BASE COUNT         1428 a         3141 c         2791 g         1388 t
ORIGIN      
        1 cgacacgcgc tcgttagcct cggcggcggt gttgaaagac cccgcctgcc cgacgtaacc
       61 gccgcccgca ggcacgcgcg ccgcggcggc gtcgttacgc acgacttgcg gcgcttcggg
      121 cgcggcgttg gaaggcgcga gcgcctgtgc gttggccggc gcgggcgttt cggcgaaacc
      181 cgcgggagac ggctcgggca cggcaaaggc gaggtcggtc gatttccggt cgggggcggg
      241 cgcggcctcc gcgcgcgacg tgtgcggcgc agcctgactg gtcgcgctgt tggggatcgc
      301 ggcgggcgct tcgcggggct gctggttggc cgcgtgattc ttgagcggct gcgacgcggc
      361 gtgttgccgc acgtcgccga accccagcca gccgaggagc acgaccagcg tgacgaggca
      421 cgccgcggcg agcgccagcg tcggcggctt gaacttcaac aggctgactt cgacggcgta
      481 cccttcgccg tcgggtcggg gtgatttgct cataagcgat ggttcggtcg tcgccgttcg
      541 gcggcggatg tcgcgcggcc ctctcgcgtc cgtaactcac ggcctcgcgc gtcgagccgc
      601 ggcggcgtga ggagtaccaa tgaagtatta aacaatccac atgccagccg aagaccccgc
      661 cgcgtcgcgc agcacgtggc cgcctccgtc aaccggaatg tttacgcgag cggcgcgtcg
      721 gcttccgcct cgaagaggcg tcgcgcgcgg agggcttcgg gcgagtcgcg gttgacaatc
      781 tttgtcaacg tgacctgact atttttgcca tttgaccgta gccggtcatt ttgcaaatgt
      841 gttcagatgt ggaacagatg attttcccgc gtctgcgcgt gcccgtcgtc cgagggtcgg
      901 cggcatgtct cttgctaact ttcgggtgcg ctcctcgcgc gtagattaag aacacccctt
      961 aatctatcca gagcaggaag gaatcagcgg gttcgcggac acgtatagcc cccaccgtct
     1021 tcagccactc tccggcccgt cgctacttcg cagacacggc gtatgataat tcttaacagt
     1081 gagcgtgcgg caaagtcgct ggcgtgggtt tcggtcgcgc tcggggcctg cgcgatagtg
     1141 ctctcggtcg gcggcgaaga cttgtggagg ccggacgcga ggctcgcgtt cctgacgctc
     1201 ttcacgctct cggtcggcgc gaccgtgctc gtgaaggtgc cgcgttcgaa agcctacgtc
     1261 gcggcctcgg acgtggccgt cttcgtcgcg ctgctgctct acgacgcttc ggtggcggtg
     1321 ccgctcgcgg cgctctccac gctcgccgcg gccaacaggt tcgaccggcg gacgacgagt
     1381 cccgcgacct ttgccgcgct cgccgcgctg gcgaccctgg ccgccgtgtg gtgcgtgaat
     1441 ctcgtgacgg cgtcggtcgc catcgcgcag cccgccgcgg tgctcgccgt caacctgctc
     1501 gcgctggccg cggcgcgcgc gctcttcggg gcggtgcccg cgttgttcat cgcgtccggc
     1561 aaggaacaac gcggcggggt cggcgcgtac gcgtccgcct ccgcgtgggg cttcgtcgtg
     1621 tacctcgtcg cggcgtcgct ggcggggctg gtcttgacgg ctgctgaatg ggtgagcgtc
     1681 gatgtctcgc tcgcggtgtt cgcggcgctg tgcgtcgcgt atcacgccta caacgtttac
     1741 gcgaagggcg gcggcgcgcg tccgggcgag ccggtcgcgg gcggtcgcgt cgcgctgcct
     1801 tcggagggcg ccgagagcct gcgcctcgcg ttcgacgacg cgccgacggg catggccatc
     1861 ctctccgcgc gcggcgagct gctgaaggtc aaccgctcct tcagccggat gctcgggcgc
     1921 acggacgcgg agctgctgct gtcgtcgggc atacaggact tcgtccacgc ggacgatctg
     1981 gggccggtgc tcgcgggcgt caagaacgtc atcaagggcg gcggctcgac cttgagcacc
     2041 gacgtgcgcc acccgctcaa ggacggtcgg atagcgtggg cgcactggaa cgtgggccgc
     2101 ttcgtcgcgc cctactccga cgaggtgcgc ttgatcctcc aggcgcagga agtaaccgag
     2161 cgcaagctcg ccgaagagaa gctgctccac gacgcctgcc acgacctgct gacgggactg
     2221 ccgaaccgcg cgctcttcat cgaccacgtc aagctcagca tctcgcgcgc gcgcaggcgc
     2281 gagggcagcc tcttcgccgt cctcttcctc gacctcgacc gcttcaagct catcaacgac
     2341 agcttgggcc acatggtcgg cgacgagctg ctggtcgtcg tcgcgcgccg gctggagcgc
     2401 tgcgtgcgcg agggcgacac ggtggcgcgc gtcggcggcg acgagttcat ggtgctcctc
     2461 gacgatttgc aggacatcgg cgaggccgtc caggtcgccg agcgcatcca gaacgaggtc
     2521 aaggttccct tcaacgtcaa cagccacgag gtcttcacca cgatgagcat cggcatcgcg
     2581 ccgtgctcgt cggagtacga caacccggaa gacatcatgc gtgacgccga cacggcgatg
     2641 taccgcgcca aatctctggg caaggcgcgc cacgaggtct tcgacaagac gatgcacgcc
     2701 ttcgccgtca acctcctgca actcgaaacc gacctccgga aggcgctcga ccgcgaccag
     2761 ttcttcatcc acttccagcc catcgtctcg ctcgacacgt tccacatctg cggcttcgag
     2821 gctttggtgc gctggcagca ccccgagcgc ggctccatct cgccgctcga cttcatcccg
     2881 gtcgccgagg agacgggcct catcgtcgcc atcggcgagt ggacgctcca cgagtcgtgc
     2941 cggcagatgc gcgagtggca gcgcgagttc cccacgcagg agccgatgtt cgtcagcgtc
     3001 aacctctcga ccaagcagtt caaccagccc aacctcatcg agacggtcgc cgacatcctc
     3061 cgcgagacgg gcctcaaccc gcgcgcgctc aagctcgaaa tcaccgagag cgccgtcatg
     3121 gagaacatcg acgtggcgac cgacatgctg cggcaactgc gcgcgctcgg cgtgcagctc
     3181 tccatcgacg acttcggcac cggctactca agtctctctt acctccaccg cttccccatc
     3241 gacacgctga agatcgaccg ctcgttcgtc acgcagatgg ccgagaacaa cgagaacacg
     3301 gagatcgtcc gcactatcgt cgtgctcgcg cagaacctcg ggatggacgt ggtcgccgag
     3361 ggcgtcgaga ccaacgacca gctcgcgctg ctgcgccagc tcggctgcga gtacgggcag
     3421 ggctactact tctcgaagcc cgtcagcagc gccgaggccg ggggcatcat cgccgacacg
     3481 cgcgccctcc aacccgtccg cgccaaaggc gacatgcggc ggctgccgcg gctcggcggc
     3541 gagccgagca cgtacacggc ggcgcccgcc gcctgagagt aggcagtagg cggtaggcag
     3601 taggcagtaa aagcaagaag ggcggagagg cttgagaggt aaagtcccga gcctcttcgc
     3661 ccttcaagca agaaaggaag aggcttgaga cgttgcatct caagcctctt ccttctgtct
     3721 ctaactgcct actgcctact tccttctcac tcgacttcga gcgcgacggg ttcatcctcc
     3781 gcgatttcga ggccgtcgcc cgcgcgcagc cttccggcct gcgccgcgcc gacgagggcg
     3841 cgcagcgcct ccgtctctcc gggcgtgggg ttgtcgaaga gcagctcgaa gccgaccttc
     3901 tccatgcagt agagcacggt cgcgcgcaag gggagccagc gctcctcggg cagccacagc
     3961 ttgacgtgca gctccatccc gtccttcgcc cacgccttcg tcttgacgaa gcagcgcttg
     4021 acgctgaggc tcgtcaccgt gcccttgcgc ggcgcgtcgt cgctcatccc gaacgagcac
     4081 gggagcgaca ccttctctct gccatcctga cctttttttg actgggtgtt catcatatcg
     4141 gtcgagtctc gaatcagtcc gttgaagtga tcggctcaag cgaccggcaa tcgtcatccg
     4201 tcccgccgca ccagttcaac gctagcaaat taaaggccgg acgctcggcg ccccgccccc
     4261 cgccccgaac tttcaccacg acggcggata aaagtccgaa cccgcgggca ctcctcagcg
     4321 ttcttggatg cgaagggtca aggggttcag tcatccgaac aacatccacg gcgccgcgca
     4381 cggttcgata aagtcacgcg cggcgcgtcc gagccggaga gagaaagcat gaactgtcgt
     4441 atgaaatgtc gcatcttcgt cgccgccgtt ctcatcatcg ccgccgccat catcgggcag
     4501 cggatgtccg ggcgccaaga cacgagcggg aacagcggcg ccgggcgcga ggagacgcgc
     4561 cggacgtacg cgctcgacgc gggcgccgag gtcgaggtct acggcatcaa cggctcggtc
     4621 gaggtcaaca ccgccgaaac gaacgccgcc gaggtctcca tcgtccgcac cgccgggagc
     4681 gccgaagacc ttgagcacag ccgcgtcgtc gtcgagcaca cgccgtcgcg cctcgtcgtc
     4741 cgcggcgagg gccacggcaa gggcggcttc tggcggtggt ttcgtggcgg cggccacgtc
     4801 aggcatcaga tcgtgctgac cgtgccgcgc ggcgtcgagc ttgagacgaa gggcatcaac
     4861 ggccccgtca ggatcggcga ggtggacggc gcggtctccg tcaacggcgt caacggtcgc
     4921 gtcgaggtgg cgcaggccgc gggccgctcc gaggtgcagg gcgtcaacgg cgcggtcacg
     4981 atcacggttg cgcgcctcgg cgccgaaggg atggaggtgc agggcgtcaa cggcagggtc
     5041 gagattcaac tgaaagaagt cctgaacgca gacatcaacg tccggaggct gaacggcagc
     5101 ttccggctcg acgcgccgaa cgtcacgatg caggagcgcg aggatcgctc gaacatgcgc
     5161 gcgcgcctcg gcaccggcgg ccccgcagtc aacatcaccg gcgtcaacgg cagcgtccgc
     5221 ttcgaatcga ccgcgaccgc cgtgaacgcg atcacgcccg tcgcgcccat cgccccggtc
     5281 gtcgtcgccg tccccgacgc tcccgacgct ccccacgcgc cgcacccgcc ggctgacgac
     5341 caccgctaag cgccccgcaa gagtttccgg cccgacctaa agcgttgtcc aaaagaagag
     5401 ggcttcacgt cggatgcgac gtgaagccct cttcgctttc acttccacca gagcaagtgc
     5461 caatggatga cacaacgcgc gcgatgattg tagggacagg gcttgtccct gcccgcgagt
     5521 tccggttgct tcgacgttag atcaaacgtg cgcgcgggcc gcggacaagc cctgccccta
     5581 caattttcat gcgcgttgca cacccatttg aaaaccgctc tcatgcgcgc ccgcctcgcc
     5641 gccttcgggg ccgtagaaga cgacccacac ggcgaggtcg gcggtgaaat tctcgaagcg
     5701 gtgccggact cccgccggga cgaagagcac gtcgcccggc ccgaacccct cgcgcgtgtc
     5761 gccgttgacg aactcgccgc tgccgctcat gacgacgtac aactcgtcgc gcgtgtgcgg
     5821 ctgttgcgcg tccacaccgc gcggcgcgta gacctcgacc acgagcgtcc cgtgttcgag
     5881 cacttcggcg aaacgctcgc cgccggggcc gggcagccgc gacatggctt cgtcgagcgt
     5941 caccctgtgt cttctctcag tcatgaaaca ccctcgcaaa cccgcgcgcg tcgaaaaaac
     6001 tgcgcgtcgc cgcggccttc ttaccgccct ctgttcagct tggagcttct taccttgatg
     6061 cgtccaacgg catctccgcc gtcaacacgc gcgccgtgcc cgtccaggag aagatgattt
     6121 tatcccgcgc gcgcaccatc tgcgggaagc tgctcggacg caccgcgccg gcgggcgcga
     6181 cggtgaccgc cgcgccgcgc ctgcccgacg gccacacgcg acgcgcgcgc agctccgcgc
     6241 ccgccttcgt cttctcgacc cagccgacga gcgcgctccc gtcttcgagc agcaagagat
     6301 cgacgcgccc cacggggtcg ccgtcgccga cctccgacgc gccgccgaac gacgcgcccg
     6361 catcgcccga gaaggcgagc cgcacactcg ggcgcccgcc cgccatcgtg taccacgcga
     6421 cggcgacgcg cctcccgctg gccgcgaccg aagggccgtt gaccgggcac gcgtcgatct
     6481 tccagccgtc cgcgtgcacc gttcgcggct tcgtccaccc gccgccgcgc gcgcgccgca
     6541 cgatcgagat gtctctcgtc tcgccttcca cgcggtcgcg gtagacgacg accacccctt
     6601 ccgaagtcaa cgccgccgaa gtctggcagc actcgcacac gcgcgcgtcg agcacctctt
     6661 cgtccgccag agcgccgtcg cgcgcgagcg tcgcgtggcg cagtgtcatt tcgccgtgag
     6721 cgtgcccgcc cgcgccgccc gccggtttca tctcgcgccc gtcgagccag acggcgccca
     6781 accggtcgcc cgccgccgag aacatcgaga cgaacccgtg ctcggtctgc gtcccgtcgc
     6841 ggtgcggcgt cacggcctcg ccccacgtcc gcccgccgtc gaacgagcgc gcgagcctca
     6901 cgtcgaacgc gtacgtgccc ggcccgttgc gcaccagcca gtgcgccgcc agcgacccgt
     6961 ccgccagccc gaccacggaa gggaagtcgg cccagttgac gaaccagttc tcgccctccg
     7021 ccacggtgcg cggcgcagac caccccgcgc cctcccgcac cgcgaagcgg agcgcgaccc
     7081 gcttctccgc cagacggtcg atccagctca ggtacacgcg cccgtcacgc cccacggaca
     7141 ggaacgactg cccgctcccc tcgcccgtcg gcggcgcgat ctcacgcacc accccttgcg
     7201 ccgtcgccgc gaccgaagag acgcacacga cgacgacaac agccaccgac gccttcatca
     7261 acagcaagaa tcgaccccgc gattttttca tcatctataa aaagtgagca gtcaggagct
     7321 attagccgtc agctttcgga tcggtgactg aggtcgatac aagcgccgct ctcgtccttc
     7381 cgccagccgt caagctaaaa gctaatcgct ggcagctaaa ggctaagatt aacagattgc
     7441 gggcacggct tggaaagggt gtgcgattaa tcgtttccta tccgtgttca cccccgtctt
     7501 ccgtcagacc ctgcaagcgc cgggcggaga atttaacgtt gaggcgcgcg aggttttggt
     7561 ttaacctgtg cgccgtcagc ctcggaatcc gaaggagtcg agacatgctc tcacgcaagc
     7621 gtcccgtcgt cgtgtcgggc tggatgttgt tgctgctggc cctcgtctgc gcgcccgccg
     7681 cgtcggcgca gacgggccgc cagcagccca cgccgacgcc gcagaacccg ccgaccatca
     7741 cgttgaccgc cgacaacgag gtcgtcacgc tctgccccga caacgtctcg gtcgcgaacc
     7801 cgcgcgtgcg cctgcgggcc gtgggcaaca gccccgacgg caacccgctg cgctaccgct
     7861 ggacgacgag cggcgggcgc atcgagggcg acggcacgga cgtggtctgg gacttgtcga
     7921 acgcgcagcc cggcgtctac tcggcgaccg tgacggtcga gagcggcccc gccggcgacc
     7981 ccctctgcac ggccttcacc tcgacgcgcg tggtcgtgcg taactgcccg ccgccgcgcc
     8041 ccttctgccc caacgtctcg atgtactgcc ccgacgtggt gcagaccggc gcgcccgtca
     8101 ccttcaccgc caacacgagc ggcggcacgc ccggcgtcac ccccgtctac aactggaagg
     8161 tctcggcggg caccatcacc tccggccagg gcacgccctc catcacggtt gacacggcgg
     8221 gcctcggcgg ccagcccatc accgcctcgc tcgaagtcga gggctacaac ctcgtctgcc
     8281 gcgccacgtg ccagtcgtcc gtgcccgcac ccgtcgtcgc gcgccgggcg gacgagttcg
     8341 gcgaggtcaa ccgcgacgac gagaaggcgc gcctcgatct gttcgccatc gagatgcaga
     8401 acgagccggg cgcccggggc tacatcctcg gctacgcggg ccgcaaggcc aggcccggcg
     8461 agggccagcg ccgcgccgac cgcgcccgcg actacctcgt caacacccgc ggcatcgagt
     8521 ccacacgcgt cgtcgtcgtt gacggcggct tcgccgagac cgggcgcacc gaactctggg
     8581 tcgttccccc cggcgccacc ccgccgcgac cgagataacc gaaagcagcg ggcagtaagg
     8641 aaagaagagg tcaggaatca gaagagggaa gtaagaagag gcttgagatg caacgtctca
     8701 agcctctttc ttttactgcc tgctgcccgc tgcccgctgt tcttgctg
//
LOCUS       DBNGOH010000091         2941 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4779311, whole genome shotgun sequence.
ACCESSION   DBNGOH010000091 DBNGOH010000000
VERSION     DBNGOH010000091.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2941)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2941)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2941
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4779311"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..405)
                     /gene="sufU"
                     /locus_tag="AC159P_01715"
     CDS             complement(<1..405)
                     /gene="sufU"
                     /locus_tag="AC159P_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly sulfur transfer protein
                     SufU"
                     /protein_id="HIR3694197.1"
                     /translation="MSELSELYQQVILDHNKKPRNFKELEGANRSAEGFNPLCGDQLK
                     VFLRVDDGAVQEVSFVGSGCAISKASASMMTQSLKGKSREDALVLFEEFHRMVLGELD
                     EEAEPNHLGRLKIFGGVRDYPARVKCASLAWHT"
     gene            complement(488..1759)
                     /locus_tag="AC159P_01720"
     CDS             complement(488..1759)
                     /locus_tag="AC159P_01720"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008291528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SufS family cysteine desulfurase"
                     /protein_id="HIR3694198.1"
                     /translation="MSATETIINEAPATTDAGRFDVERVRADFPVLAQTVNGKPLVYL
                     DNAASAQVPRIVIERGAKYLQEEHSNIHRGVHYLSQRATTAYENAREKVRRFINARES
                     RECIFVRGCTEGINLVAAAYGRKFVSAGDEVIVSEMEHHSNIVPWQMLCEEKGARLRV
                     IPMNDRGELLLDEFDALLNERTKMVAVGHISNSLGTINPIKEITARAHKFGVPVLVDG
                     AQGAPHLKIDVQDLDCDFYIISGHKMFAPTGSGVVYGKAELLEQMNPYHGGGSMIRTV
                     TFEKSTYAPIPDKFEAGTPAIASQIGLGAAIDYLNSLDREAALRHEQELLSYATEKLT
                     AIEGVRVIGNARDKASVLAFVIEEVHPHDVGTILDQEGIAIRAGHHCAQPVMQHFKVP
                     ATARASFAFYNTKEEVDRLAAAVGKVIEVFA"
     gene            complement(1774..>2941)
                     /gene="sufD"
                     /locus_tag="AC159P_01725"
     CDS             complement(1774..>2941)
                     /gene="sufD"
                     /locus_tag="AC159P_01725"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HIR3694199.1"
                     /translation="VAPIARGEFQQQKVDEGAAAGHADAVARFVSAEAARSRLVFVNG
                     VYQPGLSDLSALPEGVVVADIAAARGGESAPLLRDHLSRSDAHGEDAFGALNTAFFGA
                     GAFLYVPEKVKAEAPIQFLFLSAEAESPTAAYPRVLVVAGRGSEATVIETFASVTEAT
                     YFTNAVTAVFVADNARVNHYKVQRESERAFHVASTRAELGRDASYDLTTVTLGAKLSR
                     HNVSVRLDNAGAECWVDGLYLVGEGQHADTHSLIDHRQPHCTSHQNYKGILDGKSRAV
                     FNGRVYVHRDAQRTDAVQSNKNLLLSPDARVDTKPQLEIYADDVKCAHGATVGQLEEE
                     ELFYLLSRGLHTELARNLLTYGFAEEIVEKIRIDSIRAQLDEAILNRLHARLEV"
BASE COUNT          452 a          944 c          989 g          556 t
ORIGIN      
        1 ggtgtgccac gcgaggctcg cgcacttgac gcgcgcgggg tagtcgcgca cgccgccgaa
       61 gattttcagg cgcccgaggt ggttcggctc ggcctcctcg tccagctcgc cgagcaccat
      121 gcggtgaaac tcttcgaaga gcacgagcgc atcctcgcgg ctcttcccct tgaggctctg
      181 cgtcatcatg ctcgccgacg ccttcgagat ggcgcagccc gacccgacga agctgacttc
      241 ctgcaccgcg ccgtcgtcca cgcgcaggaa gaccttcaac tggtcgccgc acagcgggtt
      301 gaacccttcg gcggagcggt tcgcgccctc aagctccttg aagttgcgcg gcttcttgtt
      361 gtggtcgagg atgacctgct ggtaaagctc actgagttca gacatattcg aaaaccgtga
      421 accgtaagcc gtgaaccgta acaagtaaga agcaggtttt tccttgtcac ggtttacggc
      481 ttacggttta cgcgaagact tcgatcacct tccccaccgc ggcggcgagg cggtcaacct
      541 cctccttcgt gttgtagaag gcgaaggagg cgcgcgccgt ggccggcacc ttgaagtgct
      601 gcatgacggg ctgcgcgcag tggtggccgg cgcggatggc gatgccttct tggtcgagga
      661 tggtgcccac gtcgtgcggg tgcacctctt cgatgacgaa ggcgaggacg ctcgccttgt
      721 cgcgcgcgtt gccgatgacc cggacgccct cgatggccgt cagcttctcg gtcgcgtaag
      781 acagaagctc ctgctcgtga cgcagagccg cctcgcggtc gaggctgttg aggtaatcga
      841 tcgcggcgcc gagtccgatc tgcgaggcaa tggccggcgt gcccgcctcg aacttgtcgg
      901 ggatgggcgc gtaggtgctc ttctcgaacg tcacggtgcg gatcatcgag ccgccgccgt
      961 ggtacgggtt catctgctcc agcagctcgg ccttgccgta gacgacgccg ctccccgtgg
     1021 gcgcgaacat cttgtggccg gagatgatgt agaagtcgca gtccaaatcc tgcacgtcga
     1081 tcttgaggtg cggcgccccc tgcgcgccgt ccacgaggac gggaacgccg aacttgtgcg
     1141 cgcgcgccgt gatctctttg atggggttga tggtgccgag cgagttcgaa atgtggccga
     1201 cggcgaccat cttcgtccgc tcattcaaga gcgcgtcgaa ctcgtcgagc agcagttcgc
     1261 ctcggtcgtt catcgggatg acgcgcaggc gcgcgccctt ctcctcgcag agcatctgcc
     1321 atggcacgat gttcgagtgg tgctccatct cggagacgat gacctcgtcg cctgccgaga
     1381 cgaacttgcg cccgtaggcc gcggcgacga ggttgatgcc ttcggtgcag ccgcggacga
     1441 agatgcattc gcgcgactcg cgtgcgttga tgaagcggcg caccttctcg cgcgcgttct
     1501 cgtaggcggt cgtcgcacgc tggctcaggt aatgcacgcc gcggtggatg ttcgaatgct
     1561 cctcttgcag gtacttcgcg ccgcgctcga tgacgatgcg cggcacctgc gccgaggcgg
     1621 cgttgtcgag gtagaccaag ggcttcccgt tgacggtctg cgcgaggacg gggaagtccg
     1681 cgcggacgcg ctccacgtcg aaacgccccg cgtcggtcgt ggccggcgct tcgttgatga
     1741 tggtttcggt ggctgacatc ttgagcagtc cttttaaact tcgagccgcg cgtgcaggcg
     1801 gttcagaatc gcctcgtcga gctgcgcgcg gatcgagtcg atcctgatct tctcgacgat
     1861 ctcctcggcg aagccgtagg tcagcaggtt gcgcgccagc tccgtgtgca gcccgcggct
     1921 gaggaggtag aacagctcct cctcttcgag ctgcccgacg gtcgcgccgt gcgcgcactt
     1981 cacgtcgtcg gcgtaaatct caagctgcgg cttggtgtcc acgcgtgcgt ccggagagag
     2041 cagcagattc ttgttgctct gcacggcgtc cgttcgctgc gcgtcgcggt ggacgtagac
     2101 cctgccgttg aagacggcgc gcgacttgcc gtcgaggatg cctttgtagt tctggtggct
     2161 cgtgcagtgc ggctggcggt ggtcgatgag cgagtgcgtg tccgcgtgct gcccttcgcc
     2221 gacgaggtag aggccgtcca cccagcattc ggcgcccgcg ttgtccagcc gcaccgagac
     2281 gttgtggcgc gagagcttcg cgccgagcgt cacggtcgtc aaatcgtacg aggcgtccct
     2341 gccgagttcg gcgcgcgtcg aggcgacgtg gaaggcgcgc tcgctctcgc gctggacttt
     2401 gtaatggttc acgcgcgcgt tgtccgcgac gaagaccgct gtgacggcgt tcgtgaagta
     2461 ggtcgcctcc gtaacggacg cgaaggtttc gataacggtc gcctcgctcc cgcgccccgc
     2521 cacgacgagc acgcgcgggt aagcggcggt cgggctttcg gcctccgccg agaggaagag
     2581 aaactgaatc ggcgcctcgg ctttaacttt ctccggcacg tagaggaaag cgcccgcgcc
     2641 gaagaaggcc gtgttgagcg cgccgaaagc atcctcgccg tgtgcgtcgc tgcgcgagag
     2701 gtggtcgcgc agcagcggcg ccgactcgcc gccgcgcgcc gccgcgatgt ccgcgacgac
     2761 gaccccctcg ggcagcgccg acaagtccga gaggcccggc tggtagacgc cgttcacgaa
     2821 gacgaggcgg ctccgcgcag cctccgcgct gacgaaccgc gcgaccgcgt ctgcgtgccc
     2881 ggcagcggcg ccttcatcaa ccttctgctg ctgaaactcg ccgcgcgcaa tcggcgcaac
     2941 g
//
LOCUS       DBNGOH010000092         2290 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_13352, whole genome shotgun sequence.
ACCESSION   DBNGOH010000092 DBNGOH010000000
VERSION     DBNGOH010000092.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2290)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2290)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2290
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_13352"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            97..1152
                     /locus_tag="AC159P_01730"
     CDS             97..1152
                     /locus_tag="AC159P_01730"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR3694200.1"
                     /translation="MTIRNVLICTTQVPFTRGGAESHVEGLRRAFVEAGYDVEVAALP
                     FKWYPPEEIMRGALAWRLLDVCEANGKPVDLVVGMKFPAYVVAHHRKVLWVMHQYRAA
                     YNLAGTPFDDLAHYPEGPRVREWIRECDGRFIGEARKVFANSKTVAERLLRYNRIESE
                     PLYHPPPRADKLREGEQGDYVFYPSRLEPQKRQELLIEALAHTRTPVRAVFAGGTRDA
                     RHYEALVRRYKVGGRVELRGFVSEDEMVELYANALGVCYLPFDEDYGYVTLEGMLSGK
                     PVVVARDGGGATEFVDDASEGLVIEPDPRALAEALDALYADRDRARRMGARARAKLLA
                     ANLSWRNVVEKIISAAG"
     gene            1170..1994
                     /locus_tag="AC159P_01735"
     CDS             1170..1994
                     /locus_tag="AC159P_01735"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014809538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR3694201.1"
                     /translation="MLNGRKIIVVMPAYNAAKTLRQTYDDLPFETVDEVLLVDDGSSD
                     ATVELAQEMGLTTFLHKRNMGYGKNQKTCYREALKRGADIVVMVHPDYQYSPRLVLPL
                     AGMIAFGEYDAVIASRILGNGALVGGMPLYKYVSNRFLTAIQNILIGQKLSEYHTGFR
                     AFSREVLEALPLEENTNDFVFDNEMLAQVIYFRYRIGEISCPTRYFEEASSISFARSV
                     TYGLGVLATSLKFRLQRMRLAAFRIFDPNGGRLLPDYYQEMRRVEGADSVPTLDPR"
     gene            complement(2007..>2290)
                     /locus_tag="AC159P_01740"
     CDS             complement(2007..>2290)
                     /locus_tag="AC159P_01740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694202.1"
                     /translation="RALVDGLKEGRETRAGVLLKIADDPRLVERESARARVLLHFFAY
                     LRRNPDDPPDTNMNGFNYWVAEVKKHGADELANAFAVSLEHRAHAGPSR"
BASE COUNT          384 a          702 c          791 g          413 t
ORIGIN      
        1 acttttgcgc ttttgtattt tcggtttgaa gtttctactt cgaaattcaa tgcttcttaa
       61 ctgcctgccg cctgctgccc gctgcccgct ttgtttatga ccatccgcaa cgtcctcatc
      121 tgcacgacgc aagtgccgtt cacgcgcggc ggggccgagt cgcacgtgga ggggttgagg
      181 cgggcgtttg tcgaggcggg ttatgatgtg gaggtggcgg cgctgccgtt caagtggtat
      241 ccgccggagg agatcatgcg gggcgcgctg gcgtggcggc tgctggacgt gtgcgaggcg
      301 aacgggaagc cggtcgatct ggtcgtcggg atgaagtttc cggcttacgt cgtggcgcat
      361 caccggaaag ttttgtgggt catgcaccag taccgcgcgg cgtacaacct cgcgggcacg
      421 ccgttcgacg acctcgcgca ctacccggaa gggccgcgcg tgcgcgagtg gatacgcgag
      481 tgcgacgggc gcttcatcgg cgaggcgcgc aaggtcttcg ccaactcgaa gacggtggcc
      541 gagcggctgc tgcgctacaa ccgcatcgag agcgagccgc tctaccaccc gccgccgcgt
      601 gccgacaaat tacgagaggg cgagcagggc gattacgttt tttacccgag ccgtctggag
      661 ccgcagaagc ggcaggagct tctgatcgag gcgctggcgc acacgcgcac gccggtgcgg
      721 gccgtcttcg cgggcggcac gcgcgacgcg cgccattacg aagcgctcgt ccgtagatac
      781 aaggtcggcg gtcgcgtcga gctgcgcggc ttcgtctccg aagatgagat ggtcgagctg
      841 tacgcgaacg cgctcggcgt ctgctacctg ccgttcgacg aggattacgg ctacgtgacg
      901 ctggagggga tgctgtcggg caagcccgtc gtggtggcgc gcgacggcgg cggcgcgacc
      961 gagttcgtcg atgacgcgag cgagggcctc gtcatcgaac ccgacccgcg cgccctcgcc
     1021 gaagcgctcg acgcgctcta cgcggatcgc gaccgcgccc gccggatggg cgcgcgggcg
     1081 cgggcgaagc tgctggcggc gaatctttct tggcggaacg tcgtggagaa gataatctct
     1141 gcggcgggct gatagcgagg tggaaggcga tgctcaacgg tcgaaaaatt atcgtcgtga
     1201 tgccggccta taacgcggcg aaaacgctcc gacaaactta cgacgacttg cccttcgaga
     1261 ccgtcgatga agtgctgctg gttgacgacg gcagttcgga cgcgaccgtc gagctggctc
     1321 aggagatggg cctgaccacg ttcctccaca agcggaacat gggctacggc aagaaccaga
     1381 agacgtgtta ccgcgaggcg ctcaagcgcg gggccgacat cgtcgtgatg gtgcaccccg
     1441 actaccagta ttcgccccgg ctcgtcctgc cgctggccgg gatgatcgcg ttcggcgagt
     1501 acgacgcggt gatcgcctcg cggattctcg ggaacggcgc gctggtgggg ggcatgcccc
     1561 tctacaagta cgtctcgaac cgcttcctca cggccatcca gaacatcctc atcgggcaaa
     1621 agctgtccga gtaccacacg ggtttccggg ctttctcgcg cgaagtgctg gaggcgctgc
     1681 cgctggagga gaacaccaac gacttcgtct tcgacaacga gatgctggct caggtgatct
     1741 atttccggta caggatcggc gagatatcct gcccgacgag atacttcgag gaggcttcgt
     1801 ccatcagctt cgcgcgcagc gtcacatacg ggctgggggt gctggccacg tcgctcaaat
     1861 tccgcctgca aaggatgcgg ctggcggcgt tccgcatctt cgacccgaac ggcggcaggc
     1921 tgctgcccga ctactatcag gagatgagaa gggtcgaggg cgccgactcc gtgccgactt
     1981 tggacccgcg gtgaagacgc gcggcttcag cgcgaaggcc cggcgtgcgc gcggtgttcg
     2041 agcgagacgg cgaacgcgtt cgccagctcg tccgcgccgt gttttttcac ctcggcgacc
     2101 cagtagttga agccgttcat gttggtgtcc ggcgggtcgt cggggttgcg gcgcaggtag
     2161 gcgaagaagt gcagcaggac gcgcgcacgc gcgctctctc gctcgacgag ccgcgggtcg
     2221 tcggcgatct tgaggagcac gcccgcgcgc gtctcgcgcc cctccttcaa cccatcgacg
     2281 agcgcccggc
//
LOCUS       DBNGOH010000093         1822 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_746765, whole genome shotgun sequence.
ACCESSION   DBNGOH010000093 DBNGOH010000000
VERSION     DBNGOH010000093.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1822)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1822)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1822
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_746765"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(22..417)
                     /locus_tag="AC159P_01745"
     CDS             complement(22..417)
                     /locus_tag="AC159P_01745"
                     /inference="COORDINATES: protein motif:HMM:NF048429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF7670 domain-containing protein"
                     /protein_id="HIR3694203.1"
                     /translation="MRGIRRGEGVALVALAWAARATSVVSIGLLLLFFVGEGFDPSGV
                     TSRQWAGLLFFPLGVVAGMIVAWWREGVGGVVTVGSLLAFYAWHVLAGDWVPRGSAFI
                     AFALPGFLFVLYGLLSRRMSEPGLATAGR"
     gene            complement(997..>1822)
                     /locus_tag="AC159P_01750"
     CDS             complement(997..>1822)
                     /locus_tag="AC159P_01750"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HIR3694204.1"
                     /translation="IVGHVDAVGERVKDFKVGERVGVAWLHRTCGSCRFCRAGRENLC
                     ESPQFTGWTIDGGFAEYAVAPAAFAYRLPEGFDDLQAAPLLCAGIIGYRALRLTGLGE
                     REEEWAGARLGIYGFGAAGHVCIQLARARGAEVYVATRDRERHQALALELGATWVGGA
                     ADEPPAALEAAIIFAPAGELVPVALRALDKGGALVLGGIHMSAIPSFEYALLYGERVV
                     RSVANNTRDDGREFLSEAARIPVRTHTETFPLERANEALIALKHDAIKGAGVLLLE"
BASE COUNT          312 a          667 c          555 g          288 t
ORIGIN      
        1 ctttctcatt tcactctccc tttagcgtcc ggcggtggcg aggcccggct cgctcatcct
       61 cctcgacagc agcccgtaga gcacgaacag gaagccgggc agcgcgaagg cgatgaaggc
      121 cgagcccctc ggcacccagt cgcccgcgag gacgtgccat gcgtagaacg ccagcaggct
      181 gccgacggtg acgacaccgc cgacgccctc ccgccaccag gcgacgatca tcccggccac
      241 caccccgagg gggaagaaca agaggcccgc ccactggcgc gaggtgacgc cagacgggtc
      301 gaagccttcg ccgacgaaga acagcaggag cagaccgatg ctgacgacgc tcgtcgccct
      361 cgcggcccac gcgagagcga cgagcgcgac gccctcacct cttctgattc cgcgcatgac
      421 tacctcccgt tacagcgtgg gagccgacga ctcaccggcc tcttcgaccg gaggctgaag
      481 tcgcctcgtg taatgcacgt gcgtcgagag gtgacgcggc caccctcgca atgaccgcgt
      541 caccttctcg cggcgcggcg cggtgtgcac cgcgctcgtc accgcaagct tgtgccatcg
      601 cctcgactac acgaaacgca acccgtgttc cgctcgaagg gagttgaagc aagggattat
      661 tggcgggcgc cgccgcgcct cgctcgcgtc ggcgcgcatg acgtgcgtgc tttttcgccg
      721 gatcgtgcgg aagtttgcgc acgcaaacct ctcggcatta ctcgcctatg aatcggctgc
      781 cgagttatcc ccggcgccct gtcacatcat cacttttcta cggaacagtc gcttcaccat
      841 atggatgtcg gtgccggtga ggagttgcgg tctcgtgccc aacacacgcc gcgccatgct
      901 ggccgcgcct gacgaggccg actgcccctg cgggcgtcag tcagcttatc gcggtaacgc
      961 gcccgccagt cggcggcggc gttcatcgtc ttcccctcat tccaacagga gcacgcccgc
     1021 gcccttgatg gcgtcatgct tgagcgcgat cagggcttcg ttcgcacgct caagcggaaa
     1081 ggtctcggtg tgcgtgcgga cgggtattct tgctgcttcg ctaaggaact cacgaccgtc
     1141 gtcgcgcgtg ttgttggcga ccgagcgcac gaccctctcg ccgtacagca gcgcgtactc
     1201 gaacgacggg atcgcgctca tgtggatgcc gccgagcacc agcgccccgc ctttgtcgag
     1261 cgcccgaagc gcgacgggca ccagctcgcc cgccggggcg aagatgatcg cggcctcgag
     1321 tgccgcgggc ggctcgtccg ccgcgccgcc gacccacgtc gcccccaact ccaaagccag
     1381 cgcctgatgt cgctcgcggt cgcgcgtcgc cacgtaaact tctgcaccgc gcgcacgcgc
     1441 gagttgaatg cacacgtgtc ccgccgcgcc gaacccgtaa atgccgagcc gcgcaccggc
     1501 ccactcttct tctcgttctc ctaaccccgt cagccgcaac gcacggtaac cgatgatgcc
     1561 cgcgcacagg agcggcgcgg cctgtagatc atcaaagcct tcgggcaggc ggtacgcgaa
     1621 cgcggcgggc gcgaccgcgt actcggcgaa gccgccgtcg attgtccagc ctgtgaactg
     1681 cggactctcg cacaaattct cgcggcccgc gcggcagaac cggcacgagc cgcacgtgcg
     1741 gtggagccac gccacgccga cgcgctcacc gaccttgaaa tctttgacac gctcgccgac
     1801 ggcatcgaca tgccccacga tc
//
LOCUS       DBNGOH010000094         2378 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1113642, whole genome shotgun sequence.
ACCESSION   DBNGOH010000094 DBNGOH010000000
VERSION     DBNGOH010000094.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2378)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2378)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2378
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1113642"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..273)
                     /locus_tag="AC159P_01755"
     CDS             complement(<1..273)
                     /locus_tag="AC159P_01755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694205.1"
                     /translation="MKLLNTLLAAAAALAALGLVGARTAVAQTSPPWVTGEYRGGDFK
                     LVYGGRAADILVSPEDFKVVGIAAGDLAADVERVTGKRPVVRAPASA"
     gene            complement(333..1652)
                     /locus_tag="AC159P_01760"
     CDS             complement(333..1652)
                     /locus_tag="AC159P_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HIR3694206.1"
                     /translation="MTIHEPRQDSDAARVADRDDRSAPIADPRELRRHVDETVAATPV
                     VDMHTHLFAPQFGAMNLWGVDELLTYHYLVAELFRFARIAPAEFWALGKARQADLIWE
                     TLFVRHTPLSEATRGVVAVLSALGLDPRARDLADAREFFRAQRAEDYLDRVLGMSRVS
                     RVVMTNDPFDENEVRQWEGGVEVDPRFEASLRIDPLLLGWDVAAQKISAQGFRVDEQL
                     GGDTFAEARRFLDKWVARMKPLYMAVSLPDDFAFPDESVTSRMLREVVLPVCREHGLS
                     FAMMIGVRRGVNPALRGAGDGLGRADAGAVERLCAAYPDNRFLVTFLSRENQHELCVS
                     ARKFSNLMPFGCWWFLNNPSIIEEITEERVELLGTSFIPQHSDARVLDQLVYKWAHSR
                     RRIADVLYRSYDALARDGRPATRAEIERDAARLFSENFDEWVGLPTR"
     gene            complement(1804..>2378)
                     /locus_tag="AC159P_01765"
     CDS             complement(1804..>2378)
                     /locus_tag="AC159P_01765"
                     /inference="COORDINATES: protein motif:HMM:NF037850.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4982 domain-containing protein"
                     /protein_id="HIR3694207.1"
                     /translation="TPRDLGPHEENVEVYSNCEQVELFLNGKSLGTKARPSDDAPRTW
                     KVAFEPGTLRAVGSNGGKTSATHELRTAGKPARVQLSADRARLAPAWDDVAYVTATVV
                     DANGVIVPNAADLVTFRVTGPGRVVAVDSGDNASHEPFQASERKAYQGRCFAILKANA
                     SRGRINVNASAPGLAGGSIHLAAAPPARNR"
BASE COUNT          351 a          819 c          840 g          368 t
ORIGIN      
        1 cgccgaagcc ggcgcgcgca cgaccggcct cttccccgtc acgcgttcga cgtccgcggc
       61 caaatctccc gccgcgatgc cgaccacctt gaagtcttcc ggcgagacga ggatgtccgc
      121 cgcgcgcccg ccgtacacga gcttgaagtc gccgccccgg tactcgcccg tcacccacgg
      181 cggcgacgtt tgcgcgacag ccgtgcgggc gccgacaaga ccgagcgccg cgagcgcggc
      241 ggcggcggcg agcagtgtgt tcaggagctt catgccgctt ggcttccctg ccggcccgcc
      301 gtcgagcggg cgcgcacgac cggcggccat ccctaccgtg tgggcaggcc gacccactcg
      361 tcgaagtttt ccgagaagag gcgcgcggcg tcgcgctcga tctccgcgcg cgtggcgggc
      421 cgaccgtcgc gcgcgagcgc gtcgtaagag cggtagagca cgtcggcgat acggcggcgc
      481 gagtgcgccc acttgtagac gagctggtcg agcacgcgcg cgtccgagtg ctgcgggatg
      541 aagctcgtgc cgagcagctc gacgcgctcc tccgtgatct cttcgatgat cgaggggttg
      601 ttcaggaacc accagcagcc gaacggcatc aggttcgaga atttgcgcgc cgagacgcac
      661 agctcgtgct ggttctcgcg cgagaggaag gtgacgagga agcggttgtc ggggtaggcg
      721 gcgcagagcc tttcgacggc gcccgcgtcg gcgcggccca gcccgtcgcc cgccccgcgc
      781 agcgccgggt tcacgccgcg cctgacgccg atcatcatgg cgaacgacag gccgtgctcg
      841 cggcagacgg gcagcaccac ctcgcggagc atgcggctcg tcacggactc gtcagggaag
      901 gcgaagtcgt ccggcagcga gacggccatg tagagcggct tcatgcgcgc gacccacttg
      961 tcgagaaagc gccgcgcctc cgcgaacgtg tcgccgccga gctgctcgtc cacacggaag
     1021 ccctgcgcgg agattttttg cgcggccacg tcccatccga gcagcagcgg gtcgatgcgc
     1081 agagaggctt cgaagcgcgg gtctacctcg acgccgccct cccactgcct cacctcgttc
     1141 tcgtcgaacg ggtcgttggt catgacgacc cggctgacgc gcgacatccc cagcacgcgg
     1201 tcgaggtagt cctcggcgcg ttgcgcgcgg aagaactctc gtgcgtccgc gaggtcgcgc
     1261 gcgcgcgggt cgaggccgag cgcggagagc acggcgacaa cgccgcgcgt cgcctccgac
     1321 agcggcgtgt ggcggacgaa cagcgtctcc cagatgaggt ctgcctgccg cgccttcccc
     1381 agcgcccaga actccgcggg cgcgatgcgc gcgaaccgga acagctccgc gacgaggtag
     1441 tgatacgtca gcagctcgtc cacgccccac aggttcatcg cgccgaactg cggcgcgaac
     1501 aggtgcgtgt gcatgtccac gaccggcgtt gccgcgaccg tctcatccac gtgccgccgc
     1561 agctcgcgcg ggtcggcgat gggcgcgctg cggtcgtcgc ggtctgcaac gcgcgcggca
     1621 tcggaatctt gcctcggctc atgaatcgtc ataacaaaca atcctcggac agcgatcttg
     1681 ccttttcatt cgaagaaagg cgcgtgcttc aagagtcatg cggcaggcgt ttcatgttat
     1741 gcctgttctt ccgaatagca aagcgaggcg cggcggcgtg aaggtctgcc gccccgcgcg
     1801 gtcttacctg tttcgcgcgg gcggcgcggc ggcgagatgg atggagccgc cggccaggcc
     1861 gggcgcggag gcgttgacgt tgatgcgccc gcgcgaagca ttggccttga ggatggcgaa
     1921 gcagcggccc tgataggctt tgcgctcgga cgcctggaaa ggctcgtggc tcgcgttgtc
     1981 gccgctgtcc acggcgacga cgcggcccgg ccccgtgacc ctgaacgtga cgaggtctgc
     2041 ggcgttcgga acgatcacgc cgttcgcgtc caccacggtc gcggtgacgt acgcgacatc
     2101 gtcccacgcg ggcgcgaggc gcgcgcggtc tgcggagagc tggacgcgcg ccggtttccc
     2161 ggcggtgcgc agctcgtgcg tggcgctggt cttgccgccg ttcgagccga cggcgcggag
     2221 cgtgcccggc tcgaaggcga ctttccacgt gcgcggggcg tcgtccgagg ggcgcgcctt
     2281 cgtgccgagc gacttgccgt tgaggaatag ctcgacctgc tcgcagttgc tatagacttc
     2341 gacgttctct tcgtgcggcc cgaggtcgcg cggcgtcc
//
LOCUS       DBNGOH010000095         3866 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1663302, whole genome shotgun sequence.
ACCESSION   DBNGOH010000095 DBNGOH010000000
VERSION     DBNGOH010000095.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3866)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3866)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3866
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1663302"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..630
                     /locus_tag="AC159P_01770"
     CDS             <1..630
                     /locus_tag="AC159P_01770"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3694208.1"
                     /translation="KQVRFIIATHYFSDHLAALKLFPRAEIIAHRNYLHTFSTERHRT
                     AEEEAHFVEPTMLVSDALVMKWGRYTLEIFHNPGHTMSSLSIEVPEADLLMAGDNVVG
                     NIAYFSYSTPDMARHALRRLERRGRSRLISSHQGVRDSEALANAMFYLDSLQTNVRAA
                     RQSGGEGDTVLRIPIDSCLRPELKASPYEELYHRRNLESIVHRRLFLSE"
     gene            665..1786
                     /locus_tag="AC159P_01775"
     CDS             665..1786
                     /locus_tag="AC159P_01775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694209.1"
                     /translation="MPEQQLAPAEKVHRYRCPGCGADLVFGPQDGALVCPYCGRREEI
                     ASDGASVEERSYEDYLRAFEDRLGVLAPAALEVRCETCGAIVTFTPPEVAGECDFCGG
                     KIVAQPKAADPLIAPEGVLPFRLTPDASNARVRAWIGALWFAPNALKRLAAHEAVGGV
                     YIPFWTYDAHAASSYTGQRGEHYYDTETYTERDAQGNTVTRTRQVQRTHWYPASGRVS
                     RWFDDILIPATRSLAPERLRALEPWDLADLKSYEPAYLSGYKAQRYQVDLAEGFERAK
                     ELAAAAIREDVRRDIGGDVQQIDEVSTHYSAITFKHLLLPVYVGAYRFNQKVYQVVVN
                     GRTGEVQGERPYSFWKIFFFVLTLLALVGLLVLVFGSNN"
     gene            2191..2940
                     /locus_tag="AC159P_01780"
     CDS             2191..2940
                     /locus_tag="AC159P_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HIR3694210.1"
                     /translation="MRAATQAAGFRMQIFHHSTNTIAKVSIFGGLFIVAASLWVLAEV
                     NRSSYNTGQYIERQQPVQFSHKHHVGDDGIDCRYCHTSVETSNSAGMPSTKTCMNCHS
                     QIWSESPYLEIVRKSFRENKPIEWARVHDLPDYVYFNHSIHVAKGVGCTSCHGRVDEM
                     PAVFQSTSLQMEWCLACHREPERFIRPKEQVFNMAWNIEADPKDGIPANLGNSLGENK
                     APKRLEQARQLLKDYRVRSAPVLTSCSTCHR"
     gene            2960..>3866
                     /locus_tag="AC159P_01785"
     CDS             2960..>3866
                     /locus_tag="AC159P_01785"
                     /inference="COORDINATES: protein motif:HMM:TIGR04519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAT-variant-translocated molybdopterin
                     oxidoreductase"
                     /protein_id="HIR3694211.1"
                     /translation="MHIKDTLPGLYQIRKRPLTADGVRARLAEKRGREFWQSLEELAE
                     TDGFDEFLHREFPQHASEWDERADRRTFLKLMGASLALAGLTGAGCSYQPPETIVPYV
                     QPPDGLVPGKPLYFATAMPFAGGATGLLVRSNMGRPTKVEGNPDHPASGTPERQQPAE
                     RVASTDVFAQAAILELYDPDRSQIINNRGAGRTYTNFLGEIVTQLERQREKQGAGLRF
                     LTETVTSPTLAAQLADILRRYPQARWYQYEPAGPNSAAVGARLAFGDFVSTRYNFAEA
                     DRVLALDADFLTCGPASLRYSRDFAE"
BASE COUNT          709 a         1387 c         1160 g          610 t
ORIGIN      
        1 aagcaggtgc ggttcatcat cgccacgcac tacttcagcg accacctggc ggccctgaag
       61 ctcttcccgc gggccgaaat catcgcgcac cggaactacc tccacacctt ctccaccgag
      121 cggcaccgca ccgcggagga ggaggcccac ttcgtcgagc cgacgatgct cgtctccgac
      181 gcgctcgtga tgaagtgggg ccgctacacg ctggagatat tccacaaccc cggccacacc
      241 atgagcagcc tttcgatcga ggtgcccgag gccgacctgc tcatggccgg cgataacgtc
      301 gtgggcaaca tcgcatactt ttcctactcc acccccgaca tggcccggca cgcgctccgc
      361 cggctcgagc ggagggggcg gagtcgtctc atatcgagcc accagggcgt ccgcgatagc
      421 gaggcgctcg cgaacgcgat gttctacctc gatagcttgc aaaccaacgt ccgggcggcc
      481 cggcaatcgg gcggggaagg cgacacggtt ctccggattc ccatagacag ttgcctgcgg
      541 cccgagttga aggcttcgcc gtacgaggag ctgtaccacc ggcggaacct cgaatccatc
      601 gttcaccgtc gcctcttcct ctccgaatga agacgtgcaa agcctctcca ttcactaaca
      661 ggccatgccg gaacaacaac tcgcccccgc ggaaaaagtc caccgctacc gctgccccgg
      721 ctgcggcgcc gacctcgtct tcgggccgca ggacggcgcg ctcgtgtgcc cctactgcgg
      781 gcggcgcgag gagatcgcgt cggacggcgc gagcgtcgaa gagcgttcgt acgaggacta
      841 cctgcgtgcc ttcgaagacc ggttgggggt gctcgcgccc gccgcgctcg aagtgcgttg
      901 cgagacgtgc ggggcgattg tcacgttcac gccgcccgag gtcgcgggcg agtgcgactt
      961 ctgcggcggc aagatcgtcg cgcagccgaa ggccgcagac cccctgatcg cgcccgaggg
     1021 cgtgctcccc ttccgcctca cgccggacgc gtcgaacgcg cgcgtccgcg cgtggatcgg
     1081 cgcgctctgg ttcgcgccga acgcgctcaa gcggctcgcc gcgcacgagg ccgtcggcgg
     1141 cgtctacatc cccttctgga cttacgacgc gcacgccgcg tcgagctaca cggggcagcg
     1201 cggcgagcac tactacgaca cggagaccta caccgagcgg gacgcgcagg gcaacaccgt
     1261 cacacgcacg cggcaggtgc agcgcacgca ctggtatccg gcctcgggcc gcgtctcgcg
     1321 ctggttcgac gacatcctca tccccgcgac ccgttcgctc gcgcccgaac gcctgcgcgc
     1381 gctcgagccg tgggacttgg ccgacttgaa gtcctacgag ccggcctacc tttcgggcta
     1441 caaggcgcag cgctatcagg tcgatctcgc cgaggggttc gagcgcgcga aggagctggc
     1501 cgccgccgcc atccgcgaag acgtgcggcg cgacatcggc ggcgacgtgc agcagataga
     1561 tgaagtctcg acccactact cggccatcac cttcaagcac ctgctgctgc ccgtctacgt
     1621 cggcgcctac cgcttcaacc agaaggtcta tcaggtggtc gtcaacggtc gcaccggcga
     1681 agtgcagggc gagcgcccct acagcttctg gaaaatcttc ttcttcgtcc tcacgctcct
     1741 cgccctcgtc ggcctgctcg tgctcgtctt cggctcgaac aactgaaggg gaaaaggtcg
     1801 aaaggggaag agggaaaagg gagacagcaa gccgctcctt ctccctcttc cccctttccc
     1861 cttttgacct tttccccttc ggctcggccc cctgattaga atctttaatc cacgccgccc
     1921 cgaatttttc atatcaaaat ataatcttat gcttgcccat cgggaaaaaa agtagtatca
     1981 ctaaggctcc taaaaaagca gtaccaagct gattcggaag taagggccaa agcggttcgg
     2041 gcgtttcgtg agaaagcgta aagaccggcg acgccccacg ccgcacacgt ctacgcgcgt
     2101 cgtcgcggca ccgcgtctgc ttgcgggggt tgaacatacg ggtcagcgcg acttgccccg
     2161 tcccgcgcgc gcggccttct tgatcttgac ttgagagcag ccacacaggc ggcagggttc
     2221 aggatgcaga ttttccacca cagcaccaat acgattgcga aggtgagcat cttcggcggc
     2281 ctcttcatcg tcgccgcctc gctgtgggtg ctcgccgagg tcaaccgctc gtcctacaac
     2341 acgggccagt acatcgagcg ccagcagccc gtccagttct cgcacaagca ccacgtcggc
     2401 gacgacggca tcgactgccg ctactgccac acctcggtcg aaacgtcgaa ctccgccggg
     2461 atgccctcga ccaagacgtg catgaactgc cactcgcaaa tctggtcgga aagcccgtac
     2521 ctggagatcg tccgcaagag cttccgcgag aacaaaccca tcgagtgggc gcgcgtgcac
     2581 gacctgccgg actacgtcta cttcaaccac agcatccacg tggcgaaggg cgtcggctgc
     2641 acctcctgcc acgggcgcgt ggacgagatg cccgccgtct tccagtccac gtcgctccag
     2701 atggagtggt gtctggcgtg ccaccgcgag ccggagcgct tcatccgccc caaggagcag
     2761 gtcttcaaca tggcgtggaa catcgaggcc gacccgaagg acggaatccc ggccaacctc
     2821 ggcaacagtc tcggcgaaaa caaggcaccg aagcggctcg aacaggcgcg ccaactcctc
     2881 aaagattaca gggtgcggag cgcccccgtg ctgaccagtt gttcgacgtg ccaccggtaa
     2941 ggttcaggta aagcaagaga tgcacatcaa agacactctc cccggactct accaaatcag
     3001 gaagcggccc ctgaccgcgg acggcgtgcg cgcacggctc gccgagaagc gcgggcgcga
     3061 gttctggcag agcctcgaag agctggccga aacggatggg ttcgacgagt tcctgcaccg
     3121 cgaattcccg cagcacgcct ccgagtggga cgagcgggca gaccgccgca cgttcctcaa
     3181 gctgatgggc gcctcgctcg cgctcgccgg gctgaccggg gcgggttgct cttaccagcc
     3241 gcccgagacc atcgtccctt acgtccagcc gccggacggc ctcgtccccg gcaagcccct
     3301 gtacttcgcc accgcgatgc ccttcgcggg cggcgccacg gggctgctcg tgcgcagcaa
     3361 catgggccgc ccgacgaagg tcgaaggcaa ccccgaccac ccggcttcgg gcacgcccga
     3421 gcggcaacag cccgccgagc gcgtcgcctc gaccgacgtc ttcgcgcagg ccgccatcct
     3481 cgaactctac gacccggacc gctcgcagat catcaacaac cgcggcgcgg ggcgcaccta
     3541 caccaacttc ctcggcgaga tcgtcacgca gctcgaacgc cagcgcgaga agcagggcgc
     3601 gggcctgcgg tttttgaccg agaccgtcac ctccccgacc ctcgccgcgc agctcgccga
     3661 catcctgcgc cgctacccgc aggcgcgctg gtatcagtac gaacccgccg gcccgaacag
     3721 cgccgcggtc ggcgcgcgcc tcgccttcgg cgacttcgtc tcgacgcgct acaacttcgc
     3781 ggaggccgac cgcgtgctcg cgctcgacgc ggacttcctc acgtgcggcc ccgcctctct
     3841 gcgctactcg cgcgacttcg cggagc
//
LOCUS       DBNGOH010000096         3162 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4779466, whole genome shotgun sequence.
ACCESSION   DBNGOH010000096 DBNGOH010000000
VERSION     DBNGOH010000096.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3162)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3162)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3162
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4779466"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1872)
                     /locus_tag="AC159P_01790"
     CDS             complement(<1..1872)
                     /locus_tag="AC159P_01790"
                     /inference="COORDINATES: protein motif:HMM:NF024589.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR3694212.1"
                     /translation="MTLWIIAGLVLLCLLCVGLVVYLAVSLDIEHVRHRQRIEGEKLL
                     QLDNSDARSVAAQSPEAGEAVAEKAPPFDGAEEVFRVEDAPPEQPLAATDVADSSVAE
                     SETDVYALAWAMDEYFNRSSHPKDLLLHEKFSQGIELLRGEAYTAEDLLTYSRGPNVV
                     VGCMALEALARRAGESDITATLVAGLNDYFYWPRYFALRALDARARRPVLAEVLARAG
                     AGWRDFVPMQSLREFAARRRAAGEEVSADEIIDYVSRHNFNDEQLNDVAGVVEELGDA
                     LAPSVGAEFALWRGERVDLEFLKSIGRVWEPGREEDGGVVLLDAVAERVRELEAALCD
                     EPPRSVLLVGDSGVGKTATLRALARRLQRERWTVFQASAADVMSGQQYIGQLEGRVQS
                     LVRQLGGKRVLWVIPNFHETLWAGRHQYSPTGVLDLVLPSMESGQLRVVGEVPSGAYE
                     QLVRLRPKLRTAMQTYRVAPLGDAETLELGRRWAERAATVDEGAAVETNGGVAVQRLS
                     HQPSRRISEETLQEAFQLAKQYLGDKAAPGNLLVFLEATHRRLLAEGGTAAAREITLD
                     DLLVTLSQLTGLPVSILDEREGLDLASLRAFFERRVLGQPEAVDCLVERVAMIKAGLT
                     "
     gene            complement(1985..2530)
                     /locus_tag="AC159P_01795"
     CDS             complement(1985..2530)
                     /locus_tag="AC159P_01795"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3694213.1"
                     /translation="MQIFIRAPQVVDRDEFLALMRASVRFHRRWAAPPVTPEQFDEYL
                     ERCRRPNFVGLLACRREDGRIVGVFNLSEIVRGGFQSCYMGYYVGAGYQQRGYMTEAL
                     RLVLGHAFETLRLHRVEANIQPENAPSIALVRRAGFAREGFSRRYLKIAGRWRDHERW
                     ALLAEDWRKNKSEVGSRKSEG"
BASE COUNT          537 a         1103 c          999 g          523 t
ORIGIN      
        1 ggtcagcccc gccttgatca tcgccacgcg ctcgacgaga caatcgaccg cctcgggctg
       61 gccgagcacg cgccgctcga agaacgcgcg cagcgaggcg aggtcgagac cctcgcgctc
      121 gtcgaggatg ctcaccggca accccgtcaa ctgagagagc gtgacgagca ggtcgtcgag
      181 cgtgatctcg cgcgccgccg ccgtcccgcc ctccgcaagg agacgcctgt gcgtcgcctc
      241 taagaagacg agcagattgc ccggcgcggc cttgtcgccg aggtactgtt tcgcgagttg
      301 gaacgcctcc tgaagcgtct cttccgagat gcgccgcgaa ggttgatggg agagccgctg
      361 tacggcaacc cctccgttcg tctcgacagc ggctccttca tcaaccgtcg ccgcgcgctc
      421 tgcccaacgt ctgccgagtt cgagcgtctc cgcgtcgccg agcggcgcga cgcggtatgt
      481 ctgcatcgcg gtgcggagtt tgggtcgcag gcgcacgagc tgttcgtacg cgcccgacgg
      541 cacttcgccg acgacgcgca actgcccgct ctccatcgaa ggcagcacga ggtcgagcac
      601 gcccgtcggg ctgtactggt ggcggcccgc ccacagcgtc tcgtggaaat tcgggatgac
      661 ccacagcacg cgcttgccgc cgagctgccg cacgagcgac tgcacgcggc cttcgagctg
      721 tccgatgtat tgctgccccg acatcacgtc cgccgcgctc gcctgaaaga ccgtccagcg
      781 ctcccgctgc aagcgccgcg cgagggcgcg caacgtcgcc gtcttcccca cgcccgaatc
      841 gccgacgagc aagaccgacc gcggcggctc gtcgcaaagg gccgcctcca actcgcgcac
      901 gcgctcggcg accgcgtcga gcagcacgac cccgccgtct tcctcgcggc ccggctccca
      961 cacgcgaccg atggacttca ggaattcgag atcgacgcgc tcgccgcgcc agagcgcgaa
     1021 ctccgcgccg accgacggcg cgagcgcgtc gcccaactct tcgacgacgc ccgccacgtc
     1081 gttcaactgc tcgtcgttga agttgtggcg cgagacgtag tcgatgattt cgtccgccga
     1141 cacctcctcg cccgcggccc ggcggcgcgc ggcgaattcg cgcagagact gcatgggtac
     1201 gaagtcgcgc cagcccgcgc ccgcgcgcgc cagcacctct gcgaggacgg gccgccgcgc
     1261 ccgcgcgtcc agcgcgcgca gcgcgaagta gcgcggccag tagaagtagt cgttcaggcc
     1321 cgcgacgagc gtcgccgtga tgtcgctctc gcccgcgcgc cgcgcgagcg cttcgagcgc
     1381 catgcagccg acgaccacgt tcggcccgcg cgagtaggtc aaaaggtctt ccgccgtgta
     1441 cgcctcgccg cgaagaagct cgatgccctg cgaaaacttc tcgtgcagca acagatcttt
     1501 cgggtgcgag ctgcggttga agtattcgtc catcgcccat gcgagcgcgt agacatccgt
     1561 ctcggactcg gcgaccgagg agtccgcaac atcggtcgcc gccaaaggct gttccggcgg
     1621 agcgtcttcg actcgaaaga cctcttcggc cccgtcaaac ggcggcgctt tctccgcgac
     1681 ggcttccccc gcctccggcg actgcgcggc gacgcttcgc gcgtcactgt tgtcgagttg
     1741 caagagcttc tcgccctcga tccgctgtct gtggcgcacg tgctcgatgt cgagcgagac
     1801 ggcgaggtag acgaccagcc ccacgcagag caggcacagc aacaccagac cggcaatgat
     1861 ccacagagtc atcttgagct tcagccgagc atcgggaatc gagacgggga ggcgacaaca
     1921 agtcggaagt cggaaggatg aaggatgaaa cggaaggcgt caactcaaga gacgcgcgcc
     1981 tctttcatcc ttccgacttc cgacttccga cttctgactt gttcttccgc cagtcctcgg
     2041 cgaggagcgc ccagcgttcg tggtcgcgcc agcgtccggc gattttgagg tagcggcgcg
     2101 agaagccttc gcgggcaaag ccggcgcggc ggacgagcgc gatggagggg gcgttctccg
     2161 gctggatgtt ggcctcgacg cggtgcagcc ggagcgtctc gaaggcgtgg ccgaggacga
     2221 gccgcagcgc ctcggtcatg tagccgcgct gctggtagcc cgcgccgacg tagtagccca
     2281 tgtagcagct ctggaaaccg ccgcgcacga tctcgctcag gttgaagacg ccgacgatgc
     2341 gcccgtcctc ccggcggcac gcgagcagcc cgacgaagtt cggacggcgg cagcgctcaa
     2401 ggtattcgtc gaattgttcc ggcgtcaccg gcggcgccgc ccaacgccgg tgaaagcgca
     2461 cgctcgcgcg catcagcgcc agaaattcgt cgcggtcgac gacctgcggc gcgcggataa
     2521 aaatctgcat gccagcacga ataacgccgc ctcgcccgct ctcactcgat gatgtcattc
     2581 aggaaggtca gggggacggg cgtgtgccca taaaagaaga ttgcgccgcc ggcaacgagt
     2641 gacggacggc gcaacctttg atcctctttc gaaccgccga agcgattctc aggggattgg
     2701 cgactcctgc atgtacacgc atcggtcgcc ttgtttgtat cccgacgagg agcgctgaag
     2761 cggtgagcac ttcgccggtc ggcacggatt cgttttcacg tttcgttcac gcctccgaag
     2821 aggcgcggtg cctattggtt tttgttctgg tggcttttcc tccacctcca gtctacgttc
     2881 tggacaacga ggttcagccg aagctgaacg acatcgcttc tccgagttcg atcaactctc
     2941 ggagcgcctg cgcgttttta tccgcgccgc tcgggagcgc ctgccggcac atccctagca
     3001 agcctttcgg cttcggcgtc gggcgtcaat ggcgaccaat aaaaccgttc tttcggtcgc
     3061 cctcagcggt cgcgtcaccg cgtccgttgc cgatccctac gccggtcacg gcccccttga
     3121 gaaccgcgac gggaggcgaa ctatacgcac gtcggcgcaa cc
//
LOCUS       DBNGOH010000097         6709 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1296877, whole genome shotgun sequence.
ACCESSION   DBNGOH010000097 DBNGOH010000000
VERSION     DBNGOH010000097.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6709)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6709)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6709
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1296877"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(35..1426)
                     /gene="sthA"
                     /locus_tag="AC159P_01800"
     CDS             complement(35..1426)
                     /gene="sthA"
                     /locus_tag="AC159P_01800"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (Si-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HIR3694214.1"
                     /translation="MQTYDLIVIGSGPAGQRAAIQGAKLGKRVALVEKREVVGGACIN
                     TGTIPSKSMREAVLHLSGYQYHGIYGMSYRVKEKISVADLSFRVQQVIKTEIDVTSAQ
                     LSRNGIEMLTGTASFIDPHHLRIQNATGQSDYEGGIIIIATGTRPAESPLVPINGRTI
                     ISSDQIFSLPSIPKTLIVVGGGVIGVENASMFAALGVRVIVIEKQPRLLGFVDTEMVE
                     ALCYHLRDNRVTLRLNEEVKSVEETADGTVVANLESNKRISGDALLYSIGRQGNVDDL
                     NLAAAGLEADSRGRIAVDGQYRTAQPHILAVGDVIGFPSLASVSMEQGRIAVGRALGA
                     NVQSDPANYPYGIYTIPEISFVGKTEEQLTDEDVPYEVGVAFYRESARGQIRGDTTGR
                     LKLIFHRETKELLGVHIIGEGASEILHIGQAVLILKGTVDYFINTVFNYPTLAECYKA
                     AAFNGLNKLTRFS"
     gene            complement(1570..1836)
                     /locus_tag="AC159P_01805"
     CDS             complement(1570..1836)
                     /locus_tag="AC159P_01805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694215.1"
                     /translation="MPDAPLQIDLGAVETLIGTLRFTFVVSAEGDAYVQVDAGDPTNA
                     GRGGAMLMLDANGYEKLKAIIKGADDVIDRLIREGRIKRMVLPY"
     gene            complement(1900..2430)
                     /locus_tag="AC159P_01810"
     CDS             complement(1900..2430)
                     /locus_tag="AC159P_01810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694216.1"
                     /translation="MRRLIFSALLVLASLSAAWAQDLSAVEQQVERLRAQLREAADKE
                     AQLRERAQRLEDDLRPENVERSVASVGTTDARALRERRRQQLEREKAAVDEQLTSLAA
                     SRARLEGAISTAEAEAVRLRAAALGASNTTTPAAPAVAPAAAPAPPAAAPAVRKRPAA
                     PRKGRPRKARKPRRRS"
     gene            complement(2465..2980)
                     /locus_tag="AC159P_01815"
     CDS             complement(2465..2980)
                     /locus_tag="AC159P_01815"
                     /inference="COORDINATES: protein motif:HMM:NF024273.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HIR3694217.1"
                     /translation="MQPKRCPEASAPDAVERLSRMEGFLREVAEHLPGDLQRRKPSRD
                     EFSLLEHVCHLRDLEREGYRVRIERILREDRPLLPDFDGARVARERNYLEESLPQALE
                     SFGAARAHNLAIVSNLLAEQLSRTGVLDPAGEITLGWLLARMCAHDEEHREQITELRK
                     HLSTVRRSPSE"
     gene            complement(3013..3771)
                     /locus_tag="AC159P_01820"
     CDS             complement(3013..3771)
                     /locus_tag="AC159P_01820"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002635265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylamine N-acetyltransferase family protein"
                     /protein_id="HIR3694218.1"
                     /translation="MDTTAYLERIDYRGPLAPTAETLRRLQVAHLLSVPFENLSIHAG
                     EPIVLDDDALFDKIVVRRRGGFCYELNGLFAALLRALGFRVEMLSAGVMSQAGEFGPE
                     FDHMALLVSLKERWLVDVGFGDSFVEPLLLDERGEQAQGARAYRIDEDGDRLTLMRRD
                     VGGAWSAQYRFGLSPHEYADYAGMCHHHQTSPESHFTRRRVCSRLTAEGRVTLSRMRL
                     ITTSGVERDERELASEEEYAGALREHFGIVLPVR"
     gene            3988..4638
                     /locus_tag="AC159P_01825"
     CDS             3988..4638
                     /locus_tag="AC159P_01825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694219.1"
                     /translation="MRARLSQVVICVVALFGSTAVAQNVERRQTNPPPQAQEQPPNAA
                     PIDAVSNEISLLRKSLQTLNARLREISDKLFAPDAKPGGPSDKTEDRISRNLDLLSRT
                     EQRAEVLRKQLIELIEKETAFKTRLVQVEEDLRPDSIDRAISLVGSTRTPELRDVRRR
                     VLENERKGVESLLNQTSQSRLRLEEDVKQADALVYRLRQRLLPLIEKEIDKINPAQ"
     gene            complement(4706..5332)
                     /locus_tag="AC159P_01830"
     CDS             complement(4706..5332)
                     /locus_tag="AC159P_01830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694220.1"
                     /translation="MKYCPDCGESYDDREKFCELDGTPLVAAPETVALRGGRQASPWL
                     LGAAGLAVGLSVCAVALLIYYFATRGDGADPRDAQPAGATRIAEFPPPSGAAREVPTP
                     EPDETTPTPTETPTPTPTPAQTPAPAAPESPLRMLRDAPASTDPQPGQRFVIGLHDGT
                     DIVADDAWRAADGVWYRRGSLVTLLDPARIRSIERQQQQQQQQQTPEP"
     gene            complement(5619..5924)
                     /locus_tag="AC159P_01835"
     CDS             complement(5619..5924)
                     /locus_tag="AC159P_01835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694221.1"
                     /translation="MSPVRTASGSHEFVKRPDCPSSDVLLAYAGGSVAGLRRESIKLH
                     LAHCDFCDAEARLLKQHPPAAEETRVPAKLPLSLLLFAEQALPRKPVVKKPLRKRAA"
     gene            complement(6213..6560)
                     /locus_tag="AC159P_01840"
     CDS             complement(6213..6560)
                     /locus_tag="AC159P_01840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694222.1"
                     /translation="MTLLIWFALLLAVLLCALWLRKRMLWTPLGRFFYRLGFSGANGT
                     IHDLKEGRRYVVTNSLPGYEGIAVGEVLTFRHRQFVDHAGYVLQFEEREIILKEDGDV
                     VRRVWAYLEPLRR"
BASE COUNT         1098 a         2233 c         2244 g         1134 t
ORIGIN      
        1 gtgacggacg ccgtgcgccc ggcgcacttc cctgtcaact gaatctggtc agcttattca
       61 ggccgttgaa cgccgccgcc ttgtagcact cggccagcgt ggggtagttg aagaccgtgt
      121 tgatgaagta gtccacggtg cccttcagaa tcagcaccgc ctgcccgatg tgcaggattt
      181 ccgaagcgcc ctcgccgatg atgtggacgc cgagcagttc tttcgtctcg cggtggaaga
      241 tgagcttcag ccttcccgta gtgtcgccgc gaatctggcc gcgcgcgctc tcgcggtaga
      301 aggcgacccc gacttcgtaa ggcacgtcct cgtccgtcaa ctgctcctcg gtcttcccga
      361 cgaaggagat ttcggggatg gtgtagatgc cgtaagggta gttggccggg tcggactgga
      421 cgttcgcgcc gagcgcgcgg cccaccgcga tgcgcccctg ctccatcgag accgaagcga
      481 gcgaggggaa gccgatcacg tcgccgacgg cgaggatgtg cggctgcgcc gtgcggtact
      541 ggccatccac ggcgatgcgg ccacggctgt cggcctcaag gcccgccgcc gccagattga
      601 ggtcgtccac gttcccctgc cgcccgatgg aatagagcag ggcgtcgccg gaaatccgct
      661 tgttgctttc gaggttggcg acgacggtgc cgtccgcggt ctcttcgacg ctcttgacct
      721 cctcgttcag ccgcagcgtg acgcggttgt cgcgcaggtg gtaacagagc gcctcgacca
      781 tctccgtgtc cacgaacccg agcagccgcg gctgcttctc gatgacgatg acgcgcaccc
      841 cgagggcggc gaacatcgag gcgttctcga cgccgatgac gccgccgccg acgacgatca
      901 gcgtcttcgg gatgctgggc agcgagaaaa tctggtcgct gctgatgatc gtccgcccgt
      961 tgatcgggac gagcggcgat tcggcgggcc gggtgccggt ggcgatgatg atgatgccgc
     1021 cttcgtagtc ggactgcccc gtcgcgttct ggatgcggag gtggtgcggg tcgatgaagc
     1081 tggcggtgcc cgtcagcatc tcgatgccgt tgcgcgagag ctgggccgag gtcacgtcga
     1141 tctcggtctt gattacctgc tgcacgcgga aggagaggtc ggcgaccgag attttctcct
     1201 tgacgcgata gctcatcccg tagatgccgt gatactggta gcccgacagg tgcagcacgg
     1261 cttcacgcat cgacttgctg gggatggtgc cggtgttgat gcaggccccg cccacgacct
     1321 cgcgcttctc gaccagcgcc acgcgcttgc cgagcttcgc cccctggatg gccgcgcgct
     1381 gccccgccgg gccggagccg atcacgatga ggtcgtatgt ttgcatggct gaaccgatcc
     1441 cggctgccgc ccggctgccg cccgccgctc tcgcgtgctc ttcaagtgtg actggaaacc
     1501 cgtggaatat agggccgcgg gggcggggtg tcaacggcga gacgacgggc gcggccccgc
     1561 cccgttgatt cagtaaggca gcaccatccg cttgatccgc ccctcgcgga tgaggcggtc
     1621 gatcacgtcg tccgcgccct tgatgatcgc cttgagtttc tcgtagccgt tggcgtcgag
     1681 catgagcatg gcgccgcccc gccccgcgtt cgtcggatcg cccgcgtcca cctgcacgta
     1741 ggcgtcgcct tccgcgctga ccacgaaggt gaaccggagg gtgccgatga gcgtctccac
     1801 ggcgccgagg tctatctgaa gcggggcgtc cggcatcggg gtcttctcct tattacagtt
     1861 tcactccgcc gcggcgccgc gcgggcgcgc cgggccggac taagaccgcc gccgcggctt
     1921 gcgcgccttg cgcggcctgc ccttgcgcgg ggcagccggc ctcttcctca cggcgggcgc
     1981 ggcagcgggc ggcgcgggcg cggcggcagg ggcgacggcg ggcgcggcgg gcgtcgtcgt
     2041 gttgctcgcg ccgagcgcgg cggcccgcag gcggacggcc tccgcctcgg cggtcgagat
     2101 cgccccctcc agccgggcgc ggctcgcggc caagctcgtg agctgctcgt ctaccgccgc
     2161 cttctcccgt tcgagctgct gtcggcgccg ctcgcgcagc gccctcgcgt ccgtggtgcc
     2221 gaccgaggcg acgctgcgct cgacgttctc cggcctgagg tcgtcctcaa gccgctgggc
     2281 gcgctcgcgg agttgggcct ccttgtccgc cgcctcgcgc aactgcgcgc gcaaacgttc
     2341 gacctgctgc tcgaccgcgg acaagtcctg cgcccacgcc gcggagagcg aagcgaggac
     2401 gagcaacgcg ctaaagatga gacgccgcat gagacacctt tcctccctac ccccgtacac
     2461 gttgttactc ggacggcgac cggcgcacag tcgataggtg tttccgaagc tccgtgatct
     2521 gctcccggtg ctcttcgtcg tgggcacaca tcctcgccag caaccatccg agcgtgatct
     2581 cccctgccgg atcgagaacg ccggtgcggc tgagctgctc ggccaagagg ttcgacacga
     2641 ttgcgagatt gtgcgcgcgt gccgcgccga agctctccag ggcttggggc agggactctt
     2701 cgaggtagtt ccgctcccgc gcgacccgcg ccccgtcgaa atcggggagc agaggccggt
     2761 cttcacgcag gatgcgctcg atccgcacac gatacccttc ccgttcgagg tcgcggaggt
     2821 ggcagacgtg ttcgagcagc gagaactcgt cgcggctcgg tttccggcgt tgcaggtcgc
     2881 ccggtagatg ttcggcgacc tcgcggagaa atccctccat ccggctcagg cgttcgacgg
     2941 cgtcgggcgc gctcgcttca ggacatcgct tcggctgcat acagagttca cctcctgccg
     3001 tggagagcgg cgttatcgaa ccggcaacac gatgccgaag tgctcgcgta gcgccccggc
     3061 gtactcctcc tcgctcgcca gctcgcgctc gtctctttca acgccgctcg tggtgatgag
     3121 ccgcatccgg ctgagcgtca cacgcccctc cgccgtcagc cgcgagcaga cgcgccgccg
     3181 tgtgaagtgc gattccggcg aggtctggtg atggtgacac atcccggcgt aatcggcgta
     3241 ttcgtgcgga ctcaggccga agcggtactg ggcgctccat gcgccgccca cgtctctccg
     3301 catcagcgtc aggcggtcgc cgtcctcgtc gatgcggtaa gcacgcgcgc cctgcgcctg
     3361 ctcgccgcgc tcgtccaaca acaacggctc gacgaacgag tcgccgaacc cgacatcgac
     3421 cagccaacgc tccttcaggc tgaccagcag cgccatgtgg tcgaactcag gcccgaactc
     3481 cccggcctga ctcatgacgc cggcggagag catctcgaca cggaagccga gggcgcgcag
     3541 cagcgcggcg aacagcccgt tcaactcgta gcagaagccg ccgcgccggc gcaccacgat
     3601 cttgtcgaac agagcgtcgt cgtcgaggac tatcggctct cccgcgtgga tgctcagatt
     3661 ctcgaagggg acggagagca ggtgtgccac ttgcagccgg cgcaaagtct cggcggtcgg
     3721 ggcgagcggg ccgcggtagt cgatgcgttc gaggtaagcg gtcgtgtcca cacaagcccc
     3781 cctgatgctc gttaccggtc agcgccggac tgcgcgatta tacgccctca aagccgtctt
     3841 gagatgacag acatttcgcg gcccgagagg cggtgcggcg gaatgagctt tgagcttgaa
     3901 ccccgtgggg cgataattac cgaacttcca ccgcggtgag acgtgataca gactgtccgt
     3961 gaccctcaga agataaggac tctcatcatg cgcgcaagat tgtcgcaggt cgtgatttgt
     4021 gtcgtggcgc tttttggcag caccgcggtc gcccagaacg tcgagcgccg gcaaacgaat
     4081 ccgccgcctc aggcgcaaga acaaccgccg aacgccgccc caatcgacgc ggtgtcgaat
     4141 gaaatcagcc tcctgcgaaa atctctgcaa accctgaatg cccggttgcg cgaaatcagc
     4201 gacaagctct tcgcgcccga cgccaaaccg gggggcccgt cggacaaaac ggaggatcgc
     4261 atttcgcgca acctcgacct tttaagccgc accgaacaac gcgccgaagt cctgcggaaa
     4321 cagctcatcg agctgatcga gaaggagact gcattcaaaa cccggctggt gcaggtggaa
     4381 gaagacctgc gcccagacag tatcgaccgc gcgatcagcc tggtcggctc gacccgcact
     4441 cccgaactgc gcgacgtgcg gcggcgggtg ttggagaacg agcggaaggg ggttgaaagc
     4501 ctgctgaacc agacttcgca gagccgcctc cgactcgaag aggacgtgaa acaggccgac
     4561 gcgctggtct acagactccg ccagcgcctg ctcccgctga tcgaaaaaga gatcgataag
     4621 atcaaccccg cacagtaatc tgctaagccg cgcgccgtcg gcctcagccc gcgggcgccg
     4681 ccgacccggc ctcttctccg acggttcatg gctccggagt ttgttgttgc tgctgttgct
     4741 gctgttgccg ctcgatcgag cggatgcggg cggggtcgag cagtgtgacg aggctcccgc
     4801 ggcgatacca gacgccgtcc gccgcccgcc acgcgtcgtc ggcgacgatg tccgtgccgt
     4861 catgcaggcc gatgacgaag cgttggcccg gctgcgggtc ggtcgaggcg ggggcgtcgc
     4921 gcagcatcct gaggggcgac tcgggcgcgg cgggtgcggg cgtctgcgcg ggggtcggcg
     4981 tcggcgtcgg cgtctcggtg ggagtcggcg tggtctcgtc cggctcgggc gtcggcacct
     5041 cgcgcgcggc cccgcttggc ggggggaact ccgcgatgcg ggtcgccccc gccggttgag
     5101 cgtcacgcgg gtcggccccg tcgccccgcg tcgcgaagta gtagatcagg agggcgaccg
     5161 cgcacacgct caacccgacg gcgagtcccg cagcccccaa cagccacggc gacgcttgcc
     5221 gcccgccccg caaggcgacc gtctctgggg cggcgacgag cggcgtcccg tccagctcgc
     5281 agaacttctc cctgtcgtcg tagctctctc cgcagtcagg acaatacttc ataagaccct
     5341 ttcgggggcg catcgcggcg gcgcggcgcc cggagcatca taccaccgga gcggcggttg
     5401 acgtgcttcg gcaggaagag caaaggcacg cccgtcaaga cggagagggc gacgcggcga
     5461 acgaggtctg cccgcacttg tgccctgtgc gcgagaccga tgaaatgcgc gggggagggt
     5521 cgtcaacggc gagctatcga ggctcggata gagagggcga ggttcggcct cgccctctcg
     5581 tttcgggcgg tggtctgcgg tgtccgaccg tcgccgggct acgcggcccg cttccgcaaa
     5641 ggctttttca caaccggctt cctcggcaat gcctgctcgg cgaagagcag gagggataaa
     5701 ggtagcttgg cggggacgcg agtctcttcc gcggcgggcg ggtgctgctt caataagcgc
     5761 gcctcggcgt cgcaaaaatc gcagtgggcg aggtgcagtt tgatgctctc gcgccgcagg
     5821 ccggcgaccg accctccggc gtaggcgagc agcacgtcgg aggaggggca gtcgggccgc
     5881 ttgacgaact cgtgactgcc cgatgcagtg cgaacaggag acattcctac actccttcgc
     5941 ttcgattcga tttgttttgg gacgaatggg gaagggtctc ggttcagccg cggtgttcag
     6001 gctcagactc gaaccccctc ccgccgagag acggcgatgc gccaggccaa caacgggggg
     6061 gccggtctct gcctcgatgc cgcgcagatt agcaccgcgc cttcgcggcg gcaagcgaat
     6121 tatcgctccg atcagtcgcg gcgttccgtg cggcggggtt cgaagggcga agtccgagca
     6181 ggggttcgaa gtgcgcgaag aggggtcggg cgtcaccgcc gcagaggctc aaggtaggcc
     6241 cagaccctcc gcaccacgtc gccgtcctcc ttcaggatga tctccctctc ctcgaattgc
     6301 aggacgtagc cggcatgatc gacgaattgc cggtgccgga acgtgagcac ctcgccgacg
     6361 gcgatgccct cgtagccggg aagactgttc gtgacgacat agcgccgccc ttccttcagg
     6421 tcatggatcg tcccgttcgc gccggagaag ccgagcctgt aaaagaaccg accgagcggc
     6481 gtccagagca tcctctttct gagccacagg gcgcagagca ggacggcgag caacagggcg
     6541 aaccagatga gtagcgtcat gaccgattac gacttctgcc gcattcactc tccaatcgga
     6601 tgcacgcccg cgatggcggc agggattaca aggccggtat ggctaaacca gtaacacgga
     6661 ttcagaacac taccgcaact tcgaacgggt tgcctgcccc cgaccaatc
//
LOCUS       DBNGOH010000098         5730 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_747009, whole genome shotgun sequence.
ACCESSION   DBNGOH010000098 DBNGOH010000000
VERSION     DBNGOH010000098.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5730)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5730)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5730
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_747009"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            85..3447
                     /locus_tag="AC159P_01845"
     CDS             85..3447
                     /locus_tag="AC159P_01845"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3694223.1"
                     /translation="MPSDRVRAVAQGPDGAMWFGTDAGLARYDGRRILTASEEGLGAR
                     RVLALRFDEAGALWVGTENGALVRLPSGEYRALSETTGKSVTAVITPERGRALLATGD
                     GIVFEARTDGRGALAVSTLAEATTQRSTTRDGATREGVTRDGAETSQGGASSSSSSSA
                     RGGAAAARGLEPPWITSLAVAGEKVLAGTRGRGLLSVEGGTMREMLTRPRPFFVEALA
                     RDTEGNVFLGAQTSGADSGLFKLDAAPSLRPLKLPGATGVVTALGFGERGELFVGTDG
                     RGVFLYRGGGAPERFTFAGTAGGLRSDRVFAVFVDREGVAWFGTDRGVCRYDPKGVAV
                     EPLSSETEGNFVRSIYRTRAGRLLVGTNRGLYVRDARPPSSASTSSSSASPSTSLSSS
                     ATWRAVEEVAGKTVYAVAEDRAGALLVGTGTGLYVGLQTDARAKSAPAVKLEAEQKPK
                     EEAKPAGAAQGVEGETAAAAPEVAPEEQTGPAAQDETTGAQSGTANKSEPPARKVPEP
                     ALRGSVRAIAVFGGAVYVAAYGRGLERFDGVGRRTLVWPKASDDRRGREAVSLHADDA
                     NERLWIGTANAGAFYFDGREVKADPALAPLSTSTVWGVSGAGEWLWLATARGLYARRR
                     EGHLLEIAPGLDARGVVAVGDATGGRAPQAWCATAGGGVLKVSIDEEFGALTARLDAE
                     QGLPSQSAFALLPLEEVEGAGASTLLVGTTKGLARYEPGTVAPALTLTRVTAARTHQP
                     EELREKVLVLEYPQNGLVVDVAANASRTFPEQFQYSFLLQDRQGRAIKKKLSHDSQFQ
                     VESLPAGRYRVTARAYTLDLTPSAPLHFEFEVARAPFPRTIVSLSVLLALALALLLWG
                     YVQHRKVVRSGAELLDANRQLAAARLQLANEAESERRRIARDLHDQTLADLRRLLLLT
                     DEMQTAAADTAPNGDGPPAPPSSTVDPSELRTEIESISHEVRRICEDLSPSVLENVGF
                     AAALEWSLAAALAHLPADCKFAYDFTCDENLEERLRLTPAVQMQLYRIVQEAVSNVCR
                     HAQAARVRLRLHATAEGDLLLELEDDGRGFDPSDKKARTGRGLAGIRARAHMIDADVT
                     WTRRPGADGGGTVFTLRKTGAARQADGNNSSA"
     gene            complement(3663..4172)
                     /locus_tag="AC159P_01850"
     CDS             complement(3663..4172)
                     /locus_tag="AC159P_01850"
                     /inference="COORDINATES: protein motif:HMM:NF012790.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HIR3694224.1"
                     /translation="MSCTSRSARSRVFPSVCAALLCAALLCAAAVVFVEAGGVVAVRA
                     AAGQPQAAEVEWVAPDELKEKLSGGAPPVIIDVRSTEGYVGSDGKIRGAQHVRLRRLR
                     TRLAQSPLKDLPRDREVVTYCACPNDEAAVRAAQLFLAAGFERVRVLKGGWQAWLAAR
                     GQVEPKPRG"
     gene            complement(4239..5105)
                     /locus_tag="AC159P_01855"
     CDS             complement(4239..5105)
                     /locus_tag="AC159P_01855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694225.1"
                     /translation="MPSKPSARALLLLVACACAVSHACARRPQQQPLAADSSPRAQVN
                     ETTTPAEGPPPSTTASEEEPPPPAPDPQATSGDANAAATCGLPPPTPGEVGGAVERVF
                     KGAATIDASRRPYFVVGDFNGDSSQDVAVFVRPAPGRLAEINDELANWILVAPSRPAP
                     PRALPYPEVHARALERSRVLAEEADVLLAVIHGHEAKGWRDPQATQTYVLKDAAGDKL
                     KTRRRIEVLKARDGEPLPRLRGDVIAEDLGGQPGFLYYDGARYDWYDPRGYKPPAPTR
                     VVHGGAAGSMRH"
     gene            complement(5222..>5730)
                     /locus_tag="AC159P_01860"
     CDS             complement(5222..>5730)
                     /locus_tag="AC159P_01860"
                     /inference="COORDINATES: protein motif:HMM:NF018269.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="di-heme oxidoredictase family protein"
                     /protein_id="HIR3694226.1"
                     /translation="DEVPEPEDDGADVEAFANFMRATRAPGRGPITAAVLRGETLFQQ
                     ASCNVCHVQSITTAPAGTLINGGTFRVPAALGDEIIHPFSDFLLHNIGTGDGIVQNGP
                     PATRNMLRTPPLWGVRTRPELMHDGLSFTFNEAIQRHAGQATASRNFFNALTAAQKAD
                     MVAFLRSL"
BASE COUNT          787 a         2040 c         2087 g          816 t
ORIGIN      
        1 tttgcctttt accatccggc aggtcagtct gcgactgact cgccggcact gcaccgctgg
       61 ggcgcggtga cgctctttca cgggatgcct tcggacaggg tgcgcgcggt ggcgcagggg
      121 ccggacggcg cgatgtggtt cggcacggac gcggggctgg cgcgctacga cgggcggcgc
      181 atcctgacgg cgagcgagga gggtctgggc gcgcggcgcg tgctcgcgct caggttcgac
      241 gaggcgggcg cgctgtgggt cgggacggaa aacggcgcgc tcgtgcgtct gccgtcgggc
      301 gagtaccgtg cgctctcgga gacgaccggc aagagcgtca ccgccgtcat cacgcccgag
      361 cggggtcgcg cgctgctggc gaccggcgac ggcatcgtct tcgaggcacg cacggacggg
      421 cgcggcgcgc tcgccgtgag cacgctcgcc gaagcgacga cgcaacgcag caccacccgc
      481 gacggcgcga cgcgagaagg cgtgacgaga gacggcgcgg agacttcgca aggcggcgct
      541 tcgtcatcgt catcatcatc ggcgcgcggc ggcgcggcgg cggcgcgcgg gcttgagccg
      601 ccgtggatca cgagcctcgc ggtggcgggc gagaaggtgc tggcgggcac gcgcgggcgc
      661 ggcctgctct cggtcgaggg agggacgatg cgagagatgc tgacgaggcc gcgtccgttc
      721 ttcgtcgagg cgctggcgcg cgacacggaa ggcaacgtct tcctcggcgc gcagacctcc
      781 ggcgcggaca gcggcctctt caaactcgac gcggctcctt ctttgcggcc actgaagttg
      841 ccgggcgcga ccggcgtggt gacggcgctc ggcttcggcg aacggggcga actcttcgtc
      901 gggacggacg ggcgcggcgt cttcctctac cgcggcggcg gcgcgcccga gcgcttcacg
      961 ttcgcgggga cggcgggcgg cttgcgttcg gatcgcgtgt tcgcggtctt cgtagaccgc
     1021 gagggcgtcg cgtggttcgg cacagaccgc ggcgtctgcc gctacgaccc gaagggcgtg
     1081 gcggtcgaac cgctctcttc ggagacggaa ggcaacttcg tgcgctcgat ctaccgcacc
     1141 cgcgcgggcc gcctgctcgt cggcaccaac cgcggcctct acgtgcgcga cgcgcgcccg
     1201 ccctcgtccg catcgacttc atcttcgtca gcgtcgcctt caacatcgct gtcttcgtcc
     1261 gccacgtggc gcgcggtcga agaagtcgcg ggcaagaccg tctacgccgt ggccgaagac
     1321 cgcgcgggcg cgctgctcgt cggcacgggc acgggcctct acgtcggctt gcagaccgac
     1381 gcgcgggcga agagcgcgcc cgccgtgaag cttgaggccg agcagaagcc gaaggaagaa
     1441 gcgaagccgg ctggcgccgc gcagggcgtc gagggcgaga ccgccgccgc cgcgcccgaa
     1501 gtcgcgcccg aagagcagac cggccccgcc gcgcaggacg aaacgacggg cgcgcaaagc
     1561 ggcacggcga ataagagcga gccgcccgcg cgcaaagtgc cggagcctgc gttgcgcggc
     1621 agcgtgcgcg ccatcgcggt gttcggcggc gcggtctacg tcgcggccta cgggcgcggg
     1681 ctggagcgct tcgacggcgt cggtcgccgc acgctcgtct ggccgaaggc gtccgacgac
     1741 cgccgcgggc gcgaggccgt cagcctccac gcggacgacg cgaacgaacg gctctggatc
     1801 ggcacggcca acgcgggcgc cttctacttc gacggcaggg aggtcaaggc agaccccgcg
     1861 ctcgcgcccc tctcgacgag cacggtctgg ggcgtcagcg gcgcgggcga atggctctgg
     1921 ctcgccacgg cgcgcggact ctacgcgcgt cgccgcgaag gccacctgct tgagatcgcg
     1981 ccgggcctcg acgcgcgcgg cgtggtcgcc gtcggcgacg cgacgggcgg gcgcgcgccg
     2041 caggcttggt gcgcgaccgc gggcggcggc gtgttgaagg tctcgatcga cgaggagttc
     2101 ggcgcgctga cggcgcggct cgacgcggag caggggttgc cgtcgcagag cgcgttcgcg
     2161 ctgctcccgc tcgaagaggt tgaaggcgcc ggtgcttcga ctctgctcgt cggcacgacg
     2221 aaggggctgg cgcgctacga accgggcacg gtcgctcccg cgcttacgct gacgcgcgtc
     2281 accgccgcgc gcacgcacca gcccgaagag ctgcgcgaga aagtcctcgt gctcgaatac
     2341 ccgcagaacg gtctggtcgt ggacgtggcc gcgaacgcga gccgcacctt ccccgagcag
     2401 tttcagtact cgttcctcct gcaagaccgg cagggtcgcg ccatcaagaa gaagctctcg
     2461 cacgactcgc agtttcaggt cgagtctttg cccgcgggcc gctaccgcgt cacggcgcgc
     2521 gcctacaccc tcgacctcac gccgtccgcc ccgctccact tcgagttcga ggtcgcgcgc
     2581 gcgcccttcc cgcgcaccat cgtctctttg tcggtgctgc tggcgctcgc gctcgccctg
     2641 ctgctctggg gctacgtgca gcaccgcaag gtcgtgcgca gcggcgccga gcttctggac
     2701 gccaaccgcc agctcgccgc cgcacgcctc cagctcgcca acgaggccga gagcgagcgc
     2761 cgccgcatcg cccgcgacct ccacgaccag acgctcgcag acctgcgccg cctcctgctc
     2821 ctcacggacg agatgcagac agccgccgcc gacaccgcgc ccaacggcga cggcccgccg
     2881 gcgcctcctt catcgaccgt agacccttcg gagctgcgca ccgagatcga atccatctcg
     2941 cacgaagtcc gccgcatctg cgaagaccta agcccttcgg tgctggagaa cgtcgggttc
     3001 gccgccgcgc tcgaatggtc tctggccgcc gcgctcgcgc atctgccggc ggattgcaag
     3061 ttcgcctacg acttcacctg cgacgagaac ctcgaagagc gtctgcggct gacgcccgcc
     3121 gtccagatgc agctctaccg catcgtgcag gaggccgtca gcaacgtctg ccgccacgcg
     3181 caggccgccc gcgtccgcct ccgcctccac gccaccgccg aaggcgacct cctcctcgaa
     3241 ctcgaagacg acgggcgcgg cttcgacccc tccgacaaga aagcacgcac gggccgcggc
     3301 ctcgcaggca tccgcgcccg cgcgcacatg atcgacgccg acgtgacatg gacgcgccgc
     3361 cccggcgcgg acggcggcgg caccgtcttc actctccgca aaaccggcgc cgcgcgacag
     3421 gcagacggca acaactcttc cgcttagagc gtctggcggt cgagttcgcc gagacgcagt
     3481 ttcgacacgg agggcacgga ggactcacag agaccacaga gaaggagatg tctctctctg
     3541 tgccctctgt gtctcctccg tgccctccgt gttggagcga aacccccgcc acactcaacg
     3601 gcacatcgct ccagaccccc gcccgccgcg accctcgtcg cagcggaccg cggcgcgcgt
     3661 ccttatcctc tgggcttcgg ctcgacctga ccgcgggcgg cgagccacgc ctgccagccg
     3721 cccttgagga cgcggacgcg ttcgaagccc gccgcgagga agagctgcgc ggcgcggacg
     3781 gcggcctcgt cgttggggca ggcgcagtag gtgacgacct cgcggtcgcg cgggaggtct
     3841 ttgagcggcg actgcgcgag ccgcgtgcgg agtcgccgca gccggacgtg ttgcgcgcct
     3901 ctgatcttgc cgtcgctgcc gacgtagcct tcggtcgagc gaacgtcgat gatgacgggc
     3961 ggcgcgccgc ccgagagttt ttccttcagc tcgtcgggcg ccacccattc cacttcggca
     4021 gcctgcggct gtccggctgc ggcgcggacg gcaacaactc cgccggcctc gacgaagacg
     4081 accgccgcgg cgcacagcag cgcggcgcac agcagcgcgg cgcacaccga ggggaacacg
     4141 cgcgaccgcg cgcttcgact tgtgcagctc atccgaatca cctcctcttc ccgcagcttc
     4201 atccgttcat ctcctcgtcg gcgcggggcg ctgccgcctc aatgccgcat cgacccggcg
     4261 gcgccgccgt ggacgaccct cgtgggcgcg gggggcttgt agccgcgcgg gtcataccag
     4321 tcgtagcggg cgccgtcgta gtagaggaag ccgggctgac cgccgaggtc ttcggcgatc
     4381 acgtcgccgc gcagtcgcgg cagcggctcc ccgtcgcggg ccttcaagac ttcgatccgc
     4441 cgccgcgtct tcagcttgtc gcccgcggcg tccttgagca cgtaggtctg cgtggcctgc
     4501 gggtcgcgcc agcccttcgc ctcgtggccg tggatgacgg cgagcagcac gtcggcctct
     4561 tcggcgagga ctcgactgcg ctccaacgcg cgggcgtgga cttccgggta gggcaaggcg
     4621 cgcggcgggg ccgggcgcga aggggcgacg agtatccagt tggccagctc gtcgttgatc
     4681 tccgcgagcc tgccgggcgc ggggcggacg aagacggcca cgtcctgcga ggagtcgccg
     4741 ttgaagtcgc cgacgacgaa gtagggccgc cggctggcgt cgatggtcgc ggcccctttg
     4801 aagacgcgtt cgaccgcgcc gccgacttcg ccgggcgtgg gcggcggaag gccgcacgtg
     4861 gccgccgcgt tcgcgtcgcc cgaagtagct tgcgggtcgg gggcgggcgg cggcggctct
     4921 tcctccgacg ccgtcgtgga cggcggcggc ccttcggctg gcgtcgtcgt ctcgttcacc
     4981 tgcgcgcgcg gcgacgagtc cgcggcgagc ggttgctgtt gtggtcttcg cgcgcacgcg
     5041 tgcgagacgg cgcaggcgca ggcgacgagc aggagcaagg cccgcgcgga cggtttgctt
     5101 ggcatggctc tccctccgga cggcggcggg gcggggtcgc ggcgtgcggg gcttcgcctc
     5161 cgtcgagcgt ggacgaagcc ccttgtgcct ttgacatgcg ggcggcgcgc gggccgccgc
     5221 cttagagcga gcgcaggaag gcgaccatgt cggccttctg cgccgcggtc agcgcgttga
     5281 agaagttgcg cgacgcggtg gcctgccccg cgtggcgctg gatcgcctcg ttgaaggtga
     5341 aggacagccc gtcgtgcatc agctcgggcc gcgtgcgcac cccccagagc ggcggcgtgc
     5401 gcaacatgtt gcgggtcgcg ggggggccgt tctggacgat gccgtcgccg gtgccgatgt
     5461 tgtgcagcag gaagtcgctg aacgggtgga tgatctcgtc gccgagtgcg gcggggacgc
     5521 ggaaggtgcc gccgttgatg agcgtgccgg cgggcgcggt cgtaatgctc tgcacgtggc
     5581 agacgttgca cgacgcctgc tggaagagcg tctcgccgcg caagaccgcc gccgtgatgg
     5641 gaccgcggcc cggcgcgcga gtggcccgca tgaagttggc gaacgcctcc acgtcggccc
     5701 cgtcgtcttc aggctcgggc acctcgtcga
//
LOCUS       DBNGOH010000099         2875 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_563664, whole genome shotgun sequence.
ACCESSION   DBNGOH010000099 DBNGOH010000000
VERSION     DBNGOH010000099.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2875)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2875)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2875
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_563664"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..159)
                     /locus_tag="AC159P_01865"
     CDS             complement(<1..159)
                     /locus_tag="AC159P_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011091418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 domain-containing protein"
                     /protein_id="HIR3694227.1"
                     /translation="MSKEDLIPAMGVIIDKQPNAFFKVQIDNSEHVVLAQISGKMRKN
                     RIRILVGDR"
     gene            complement(287..613)
                     /locus_tag="AC159P_01870"
     CDS             complement(287..613)
                     /locus_tag="AC159P_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010583389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA];
                     GO_process: GO:0006397 - mRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA recognition motif domain-containing protein"
                     /protein_id="HIR3694228.1"
                     /translation="MSMKLYVGNLSFQTSGDDLQQLFSQAGTVESATVVEDRETGRSR
                     GFGFVEMSSKEEGQAAIQQFNGKEVGGRALNVNEAKPREDRGGSRGGSGGGGRNGGGR
                     GGYSRY"
     gene            1008..1538
                     /locus_tag="AC159P_01875"
     CDS             1008..1538
                     /locus_tag="AC159P_01875"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020732528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3694229.1"
                     /translation="MENLTADSFGYSIRPLAPSDEPFLWEMLYQSLYVPPGKEPFPRE
                     VVKKPHIARYVEGWGRAHDLGFVAEDEATGRPVGAVWMRLLVGAEKGFAHLDDETPEM
                     GIALLPGYRGLGVGTALLEHLIEAARRSYPAPALSVSAHNPAKRLYERLGFEVVEVRG
                     GDRPVMRLALGGRLLE"
     gene            1553..2062
                     /locus_tag="AC159P_01880"
     CDS             1553..2062
                     /locus_tag="AC159P_01880"
                     /inference="COORDINATES: protein motif:HMM:NF017015.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HIR3694230.1"
                     /translation="MTKSYRRGPVGALMDEYERAASELSRLVERIPEEDVVRVVDPRT
                     GDEDCRSVQTITSHVVNAGYGYADYLRAVFAIPSARPPKRLLAPRESLAQLDAMLAYT
                     AETLADRWEMTDEEMTSAVIHSGWGVRYDVEQLLEHAVVHVLRHRRQIERFIARGLIR
                     VLPGADGSA"
     gene            complement(2113..2514)
                     /locus_tag="AC159P_01885"
     CDS             complement(2113..2514)
                     /locus_tag="AC159P_01885"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein"
                     /protein_id="HIR3694231.1"
                     /translation="MDSLEFKVRRSPIQGRGLFALSAIPARRKLGELGGELITEREAR
                     RRARTTGSIMIVEFGDGTALDASRTGSHFRYVNHSCTPNAFMRLFKGHVEFYALRPIA
                     AGEELTCNYGETHHEGTLPCRCGSVGCKGYL"
BASE COUNT          479 a          871 c         1016 g          509 t
ORIGIN      
        1 gcggtcgccg acgaggatgc ggatgcggtt cttgcgcatc ttgcccgaaa tctgcgccag
       61 cacgacgtgc tcgctgttat cgatctggac tttgaaaaac gcgttgggct gcttgtcgat
      121 gatgacgccc atcgccggga tcaagtcttc tttggacaat cttggttctc ctctgctggc
      181 tgacggctgt cgaacgcgcc cggaggccga cggaaaaagc ctccggcaag ggatgtcgcc
      241 ggaggctttt tcagtaaaag agtcgccggg attagcgggc gacgagtcag tagcgcgagt
      301 agccgccgcg cccgccgcca ttgcgaccgc cgccgccgga gccgccgcgg ctgccgccgc
      361 ggtcttcgcg cggcttggcc tcgttgacgt tcagcgcgcg cccgccgact tccttcccgt
      421 tgaactgctg gatggccgcc tggccctctt ccttcgaaga catttcgacg aagccgaagc
      481 cgcgcgagcg gcccgtctcg cggtcttcga ccaccgtcgc cgactcgacc gtcccggcct
      541 gcgagaacag ttgttgcaag tcgtcgccgg aggtctggaa cgagaggtta ccgacgtaga
      601 gtttcattga catggatgtg taccttctcc ttctagcagg agcgtctgag agaagctgcg
      661 aatcgaagtg gcttcgtcgg aacggtggca gcgaagtaac tgggttcgga tgctctgtta
      721 cgagcggctt ctgaagtttg aataaagccc gctctcgaac tatccagaac gccgcggcca
      781 aacaaggcgc ggggcgcatc accgtcgatg cgcgagagaa ggagtgacgc gactatgatg
      841 cctcacacga cagcctcgtt gcaagtcgcc gcggcacgcg tatactgccg acgaagcacg
      901 cgcggggcct tgaaccgcct ttgcgcgcct gtcaggcggc ctcgcgtggt caacttgtcg
      961 gccccgcggt cggttgcgcg gtggctgaat tccggcggtg tgcggcgatg gaaaatctca
     1021 cggcggactc gttcggttac agcatcagac ccctggcccc gtccgacgag ccgttcctct
     1081 gggagatgct ctaccagtcg ctctacgtcc cgccggggaa agagcctttc ccgagggagg
     1141 tcgtgaagaa gccacacatc gcgcggtacg tcgagggctg gggccgcgcg cacgatctgg
     1201 ggttcgtcgc ggaagacgag gcgaccgggc ggcccgtcgg cgccgtgtgg atgaggctgc
     1261 tcgtcggggc ggagaagggg ttcgcgcacc tcgacgacga gacgccggag atggggatcg
     1321 cgctgctgcc ggggtacaga ggtctgggcg tcgggacggc gttgctcgaa cacctgatcg
     1381 aggcggcgag gaggtcgtat ccggcgccgg cgctcagcgt ctcggcgcac aatcctgcga
     1441 agcgcctgta cgagcggctg ggcttcgagg tggtggaggt cagggggggc gaccgccccg
     1501 tgatgaggct ggcgttgggt ggccggctgt tggagtgagg aagcgggaag tcatgacgaa
     1561 gagctaccgg agaggccccg tcggggcgtt gatggacgag tacgagcgcg cggcgtcgga
     1621 actcagccgc ctggtcgagc gcatccccga agaggatgtc gtgcgggtgg ttgacccgcg
     1681 gacgggggat gaagactgcc gctcggtgca gaccatcacg tcccacgtgg tgaacgcagg
     1741 ctacggctac gccgattacc tgagggcggt gtttgccatc ccgtcggccc gccccccgaa
     1801 gagactgctc gcaccccgcg agtctttagc gcagctcgac gcgatgctcg cgtacaccgc
     1861 cgagaccctc gcggacaggt gggagatgac tgacgaggag atgacgagcg cggtcatcca
     1921 ttcggggtgg ggcgtgaggt acgacgtgga gcagttgctg gagcacgccg tcgtgcacgt
     1981 cctgcgccac cggcggcaga tagagaggtt catcgcacga gggctgatcc gcgtgcttcc
     2041 gggcgcggac ggctccgctt gaacaagcgt ctccgcgcgg gccatccgga gcgcctgcgc
     2101 agagtagccg ggttacaggt agcctttgca gccgacgctc ccgcagcgac aggggagcgt
     2161 gccttcgtgg tgcgtctcgc cgtagttgca ggtcagctcc tcgccggcgg caatcgggcg
     2221 cagcgcgtag aactcgacgt gccctttgaa gagccgcatg aaggcgttgg gcgtgcacga
     2281 gtggttgacg tagcggaagt ggctgccggt gcgcgaggcg tcgagggccg tgccgtcgcc
     2341 gaactcgacg atcatgatgg agccggtcgt gcgcgcacgc cgccgcgcct cgcgctcggt
     2401 gatgagttcc ccgccgagtt cgccgagctt cctccgcgcg gggatggccg agagcgcgaa
     2461 cagcccgcgg ccctgtatcg ggctgcgccg caccttgaat tcgagactgt ccacgcggcc
     2521 aataataaac gagtaagcgg cgccggtgga gaaaatctcc acgcgccgga aggagttcgc
     2581 ccctccgcgg ccccttcaac cggctcgata agtcaggggc gtccggcggg gcgggcccaa
     2641 agtacaagag cgcgggggcc gcgacgacag ttcgtcgcgg ccccccgcgc tcttgcttag
     2701 ccgttacttt ccgggcgagg gccggagggc tgcggttgaa gaattaagcg tactgtcaca
     2761 ttgctgatgt gactatttgt cacctacgtt ttgtgagtat ctcccagata cttgatcttg
     2821 agatatcgac cccggcggcg gttgcgccgc cgtaagtgag ctgtgatgcg gcgct
//
LOCUS       DBNGOH010000100         2823 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1663720, whole genome shotgun sequence.
ACCESSION   DBNGOH010000100 DBNGOH010000000
VERSION     DBNGOH010000100.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2823)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2823)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2823
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1663720"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..355
                     /locus_tag="AC159P_01890"
     CDS             <1..355
                     /locus_tag="AC159P_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 130 protein"
                     /protein_id="HIR3694232.1"
                     /translation="YVLYLLMTDLHEPWRVTHAPAGYFIAPEGDERVGDVSNVVFSSG
                     WVARAGGEVFIYYGSSDTRMHVATGTVERLLDYVVNTPPDALRSYACAAQRRELIARN
                     LELLRRTGMRTEGDQ"
     gene            complement(402..2219)
                     /locus_tag="AC159P_01895"
     CDS             complement(402..2219)
                     /locus_tag="AC159P_01895"
                     /inference="COORDINATES: protein motif:HMM:NF014147.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding protein"
                     /protein_id="HIR3694233.1"
                     /translation="MTNRRTGALLSLCLLLASGLLPARAQTVSVWLTTGNQRTKMQPQ
                     APVTFAAGGGGANPLVVDETQVYQQVEGFGASFTDSAAYLLNRVATPAARAAAMNNLF
                     TRGGQGIGVSFVRNPMGGSDLARFHYSYDDLPAGQTDPALVNFSVAHDQADIIPLVLQ
                     ARQLNPQLTVMASPWSPPGWMKDSGSLVGGSLLPTMYGPFANYFVKFIQAYQAAGIPI
                     DYISLQNEPLFVPGDYPGMSMDAATQTLVLRDHVLPALAANNLTTKVLVYDHNWDRPD
                     YPDAVLSDPLLFGSAQVAGTAWHGYGGTPGAMTTLHNKYPSKGNYQTEHSGGTWVGDQ
                     VGADFQEITQVMRNWGRAYVKWSLALDQNRGPHAGGCGTCHPLVFVNNSSGAVSYAID
                     FYTLGHFSKFVLPGARRVYSSNAAGVIGVAFRNPDGSKALVAYNDTKTAKTFQVQWGT
                     QVFNHTLAGLSGATFTWGGAQSGGYALSAATQIQASSFNDMRGLQTEVTTDTLGGFNV
                     GYADDGDYAVYRNVTFPAGLGTVDVRAAGAGNGGTLEFRLGGVAGPLVAAVNLPVTGG
                     WQKWTTVSAPVSGATGQHDLYVVFRGTTDIGNVNWFRFK"
     gene            complement(2301..>2823)
                     /locus_tag="AC159P_01900"
     CDS             complement(2301..>2823)
                     /locus_tag="AC159P_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020711160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fibronectin type III-like domain-contianing
                     protein"
                     /protein_id="HIR3694234.1"
                     /translation="WEDNPAHASYYPEGDTKRVVYKEGVFVGYRGYEKNGTAPLFPFG
                     HGLSYTKFQYSGLSVKPSGGATFEVSFDVKNTGAREGADVAQVYVGDTHAKVPRPAKE
                     LKGFAKVSLRPGETKRVSVRLDRRALSYYDAEGRRWRADPGDFDVLVGRSSAQIELRG
                     KLSLAAAEAADSK"
BASE COUNT          431 a          950 c          980 g          462 t
ORIGIN      
        1 ctacgtgctc tacctgctga tgaccgacct gcacgagccg tggcgcgtca cgcacgcgcc
       61 ggccggatac ttcatcgccc cggaaggcga cgagcgcgtc ggcgacgtgt cgaacgtggt
      121 cttcagcagc ggctgggtcg cgcgcgcggg cggcgaggtc ttcatctact acggctcctc
      181 cgacacgcgc atgcacgtcg ccaccggcac cgtcgaacga ctgctcgact acgtcgtgaa
      241 cacgccgccc gacgcgctcc gctcctacgc ctgcgccgcg cagaggcgcg aactcatcgc
      301 gcgcaacctt gagctgctgc ggcggacggg gatgcgaacc gagggcgatc aataaagcgg
      361 cgcgcggtcg agattagcgg agccttagcc cttccggcct tttatttgaa cctgaaccag
      421 ttcacgttcc cgatgtcggt cgtcccgcgg aagacgacat agaggtcgtg ctggccggtc
      481 gcgcccgaga ccggggcgga gacggtcgtc cacttctgcc agccgcccgt gacgggcagg
      541 ttcaccgcgg cgacgagcgg ccccgccacg ccgccgaggc ggaactccaa cgtgccgccg
      601 ttgcccgccc ccgccgcccg cacgtcaacc gtgccgaggc ccgccgggaa cgtgacattc
      661 ctgtagacgg cgtagtcgcc gtcgtccgcg tagccgacgt tgaacccgcc gagcgtgtcg
      721 gtcgtcacct cggtctgaag cccgcgcatg tcgttgaagc tcgaagcctg tatctgcgtc
      781 gccgcgctca gggcgtaacc gccgctctgc gcgccgcccc acgtgaaggt cgcgcccgac
      841 aggccggcca gcgtgtggtt gaagacctgc gtgccccact gcacctgaaa ggtcttggcg
      901 gtcttggtgt cgttgtaggc gacgagcgcc ttcgagccgt cggggttgcg gaaggccacg
      961 ccgatgacgc ccgcggcgtt cgaggagtag acgcgccgcg cgcccggcag gacgaacttg
     1021 ctgaagtggc cgagcgtgta gaagtcgatg gcgtaagaga ccgcgcccga cgagttgttg
     1081 acgaagacga gcgggtggca cgtcccgcag ccgcccgcgt gcggcccgcg gttctggtcg
     1141 agcgcgaggc tccatttgac gtatgcccgc ccccagttgc gcatcacctg cgtgatctcc
     1201 tgaaagtctg cccccacctg atcgccgacc cacgtcccgc ccgaatgctc ggtctggtag
     1261 ttgcccttcg aagggtactt gttgtgcagc gtcgtcatcg cgccgggcgt gccgccgtag
     1321 ccgtgccaag ccgtgcccgc cacctgcgcc gagccgaaga gcaaggggtc ggagaggacc
     1381 gcgtccgggt agtcgggccg atcccagttg tgatcgtaga cgagcacctt cgtcgtcagg
     1441 ttgttggcgg cgagggcggg cagcacgtgg tcgcgcagca ccagcgtttg cgtcgccgcg
     1501 tccatcgaca tgccggggta gtcgccgggg acgaagagcg gctcgttttg cagcgagatg
     1561 tagtcgatgg gaatgcccgc ggcctgatac gcctgtatga acttgacgaa gtagttggcg
     1621 aacggcccgt acatggtcgg caggagcgag ccgccgacga gagagccgga gtccttcatc
     1681 cagccgggcg gactccacgg cgaggccatg acggtgagct gcgggttgag ctgccgggcc
     1741 tgtaggacga gcgggatgat gtcggcctga tcgtgcgcga cggagaagtt gacgagggcg
     1801 gggtcggtct gccctgcggg caggtcgtcg taagagtagt ggaagcgcgc gaggtcggag
     1861 ccgcccatcg ggttgcgcac gaagctgacg ccgatgccct gcccgccgcg cgtgaacagg
     1921 ttgttcatcg ccgcggcgcg cgccgcgggc gtcgccacgc ggttcaggag gtacgcggcg
     1981 gagtccgtga acgacgcgcc gaagccttcg acctgctggt agacctgcgt ctcatcgacg
     2041 acgagcgggt tcgcgccgcc cccgcccgcc gcgaacgtca caggggcttg cggctgcatc
     2101 ttcgtccgct ggttgccggt cgtcagccag acgctcaccg tctgcgcgcg cgccggaagc
     2161 aaaccggagg ccagcagcaa gcagagcgac agcaaggcgc cggttcgtct gttcgtcatc
     2221 tcgtcgtcct cctcccgtct atcggctttg cttaaccact gacagcggcc agcggctttc
     2281 agctccggcc cgccgcaggc tcacttgctg tccgccgcct cagccgccgc gagcgagagc
     2341 ttgccgcgca gctcgatctg cgccgaggag cgcccgacga gcacgtcgaa gtcgccgggg
     2401 tctgcgcgcc agcggcggcc ttcggcgtcg tagtaggaga gggcgcggcg gtcgagccgg
     2461 acggagacgc gcttcgtctc gccgggccgc aaagaaacct tcgcgaagcc cttcagctcc
     2521 ttcgcgggcc gcggcacctt cgcgtgcgtg tcgccgacgt agacctgcgc cacgtccgcg
     2581 ccctcgcgcg cgcccgtgtt cttcacgtcg aaagagactt cgaacgtcgc gccgccggag
     2641 ggtttgacgg agaggccgct atattggaac ttggtgtagg agaggccgtg gccgaacggg
     2701 aagagcggcg ccgtgccgtt cttctcgtag ccgcggtagc cgacgaagac gccttcctta
     2761 tagacgacgc gcttcgtatc gccttccggg tagtagctcg cgtgcgcggg gttgtcctcc
     2821 cag
//
LOCUS       DBNGOH010000101         7213 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3496946, whole genome shotgun sequence.
ACCESSION   DBNGOH010000101 DBNGOH010000000
VERSION     DBNGOH010000101.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7213)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7213)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7213
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3496946"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..439)
                     /locus_tag="AC159P_01905"
     CDS             complement(<1..439)
                     /locus_tag="AC159P_01905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694235.1"
                     /translation="MSRHTYARPYMLTVIALGAVVGLFSAYLLDAARIDMPFLLLAVS
                     TVTIASRMVVRLPRVNGQITVSDTFIFLTILVYGGEAGVLLAAAEGICSSIGISRKAI
                     TYAFNAGVMAASTFLSVWAVQLFFGPVTALAGAGVSFDANFVSA"
     gene            complement(553..1470)
                     /locus_tag="AC159P_01910"
     CDS             complement(553..1470)
                     /locus_tag="AC159P_01910"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3694236.1"
                     /translation="MFSTTPFEIPGDGPHSRPAAEAPPAVLAPQPLAEEHRQEALDFL
                     AVRPLHTVFMVGLIRDNGVVSPSNRGTFHACRDRAGRLEGVALIGHHTLVEARSEEAM
                     IAFARFAQGCTLTHLIRGEQEKVELFWRHYAEGVREPRRVCRELLLELRQPVAAREAV
                     PGLRPAAPADIENVMRVNGAMAFAESGVNPLRSDAEGFRLRTLRRIEQGRVWLWAEGG
                     RLVFKADIMADTPEAVYLEGVYVRPDERGKGHGTRCLSQLGRLLLARTRTLCLVVNEQ
                     HEAARAFYVNAGFEPRGNYDTIYLRRASD"
     gene            complement(1867..2274)
                     /locus_tag="AC159P_01915"
     CDS             complement(1867..2274)
                     /locus_tag="AC159P_01915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694237.1"
                     /translation="MELSHTTRRGQGNRGERGGSGLNLLVVLLVLGAAAYCAYQYAPV
                     AYSAVLFRDFMEETVNKAAYPPGQTTTWVETQLRANAKENDLPPDMRLEVQNQNGKIV
                     AHAQWTKPIRLPGYVYEYKFDHTARSSGFINPQ"
     gene            complement(2315..3523)
                     /gene="aroB"
                     /locus_tag="AC159P_01920"
     CDS             complement(2315..3523)
                     /gene="aroB"
                     /locus_tag="AC159P_01920"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HIR3694238.1"
                     /translation="MVDDKEMRKVSVRLKRGAGYPIEIGAGALGRVGAVARERLSPHA
                     RRVAVVSNKRVFGLYGGRVVESLRGAGFDVSQWMMGEGERFKSLKTAERALRFMSEAR
                     LERSDAVVALGGGVVGDLAGFAAALYLRGVAFIQAPTTLLAQIDSSVGGKTGVNTAAG
                     KNLVGAFHHPRAVLIDTETLRTLPRRELTAGWCEAIKQGAAGDAALFRRTVEYLTQGA
                     RAGTKAARVGKRVAAGAVDEVDGRGRDEDELAALIAAQCAFKAKIVAGDEREEVGRTD
                     ARSRSILNFGHTVGHALEALTRYRRFRHGEAVGHGMLVAAELSTRLGLLGATELESLR
                     EAVRLAGRLPRADDLGPDAIRRALRADKKSVGGSVRWVLLERLGRARVVDGREVTASV
                     VRESLRAALC"
     gene            complement(3618..5144)
                     /locus_tag="AC159P_01925"
     CDS             complement(3618..5144)
                     /locus_tag="AC159P_01925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694239.1"
                     /translation="MNPSDYRRDYAAYRSAVERERYRHHAGLVALPDLEQVEERYADL
                     WTRESIDEQRRAYEESPGQFETERAGLRALAGAARLAYLEVRAREVTEELVRCEAGGR
                     IGWGGAEVAASEAPGLVAFESDAVRRRELAARWFDAVRPCEDLRAARLEALNEAAREL
                     GFDHLRALHEDVTGVGLEALTAAAEIFLERTARAYSAHLSRWAARHAPRGAAGGVHYA
                     DELFFERAAHLDVYFPAEWFGAAYAWTLAGLGVRPGGQRNVKVDAEPRPSKKERSACF
                     AVEPPKDVRLVVGARGGGAQWARETFREAGRAQSFGWASGEAAARYPEFVYAPDGATA
                     EGHALLFAGLLRDAAWLGAQSAMRATEAEEVTRTLALLELHDARRDCAGLRYWLALSS
                     AGDARSEQLKEAYLSSHADATGFRYDAATHLLDAERAARSATRLRARLFAASSREHLR
                     ARHGRRWYASRAAGDELIDVWNTASRYTVEELARLAWGGELSFDLLADELLAVLGEGV
                     "
     gene            complement(5705..7060)
                     /gene="glmM"
                     /locus_tag="AC159P_01930"
     CDS             complement(5705..7060)
                     /gene="glmM"
                     /locus_tag="AC159P_01930"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HIR3694240.1"
                     /translation="MRAAGRSLARHLTAQAGRAPRVVAGRDTRESGGWIERAFLEGAL
                     AGGAAEAVSAGVITTPGVAFLARTLPADAGVVISASHNPYQDNGIKIFAPTGRKLDEA
                     TERLIEADIHAETAGARPVSNEDCEPKTEALGEVETGTESASAGAQDEQHARALRTRY
                     LDYLVGTIAAGLRLDGLGVVVDCANGAAFELAPALFERLGARVVRLHVAPDGRNINRD
                     CGSLHIEGLRQRVSEEKADIGVAFDGDADRSLFVDARGTFVDGDATMWALAKRMSERG
                     ELEGGTVVATVMSNIGLELALRSRGIELLRTDVGDKYVLEELLRTGAVLGGEQSGHII
                     FPHLSLAGDGMLTAIQLLRVMRETGAALHELTAGFERYPQVLTNVRVREKLPFEEVGE
                     IARCARETEAKLGGRGRLLLRYSGTEPLARVMIEGERQDEIEALASALASVIRDNLGA
                     G"
BASE COUNT         1107 a         2552 c         2401 g         1153 t
ORIGIN      
        1 cggcgctgac gaagttggcg tcgaacgaga cgcccgcgcc cgccagggcc gtgaccggcc
       61 cgaagaagag ctgcacggcc cagacgctga ggaaagtcga ggcggccatc acgcccgcgt
      121 tgaaggcgta ggtgatcgcc ttgcggctga tgccgatgga ggagcagatg ccctccgccg
      181 ccgccagcag cacgcccgcc tcgccgccgt agaccagtat cgtcagaaag atgaaggtgt
      241 ccgagacggt gatctgcccg ttgacgcgcg gcagccgcac caccatccgc gaggcgatgg
      301 tcacggtcga gacggcgagc agcaggaagg gcatgtcgat ccgcgccgcg tcgagcaggt
      361 aggcggagaa caggccgacc accgcgccga gcgcgatgac cgtcaacatg tatggccggg
      421 cgtaggtgtg cctgctcatg ggtcgcgtgc gagattgcta ccggcgagtg accggtcttt
      481 cgaggagggg caacgggcga ggccggcgaa ggtgagagtc gggcgccggc ctgtcttcgc
      541 gggcgcggcg ggctagtcgc tcgcgcgccg caggtaaatc gtatcgtaat tgccgcgcgg
      601 ttcaaagccg gcgttcacgt aaaacgcgcg ggccgcttcg tgctgctcgt tgacgacgag
      661 gcagagcgtg cgcgtgcgcg cgagcaggag ccgccccaac tgcgagaggc agcgcgtccc
      721 gtggcccttg ccgcgctcgt cgggccggac gtagacgcct tcgaggtaga cggcctcggg
      781 cgtgtcggcc atgatgtcgg ccttgaagac gagccgcccg ccctcggccc acagccacac
      841 gcgcccctgc tcgatgcggc gcagcgtgcg gaggcggaag ccttcggcgt cgctgcggag
      901 cgggttgacg ccgctctcgg cgaacgccat cgcgccgttc acgcgcatca cgttctcgat
      961 gtcggcgggc gcggcgggcc gaaggcccgg caccgcctcg cgcgcggcga cgggctgacg
     1021 cagctccaac agcaactcgc ggcagacgcg gcgcggctcg cgcacgcctt cggcgtagtg
     1081 ccgccagaac agctcgacct tctcctgctc gccgcggatg aggtgcgtca gcgtgcaccc
     1141 ttgcgcgaag cgcgcgaagg cgatcatggc ctcttcgctg cgcgcctcga cgagcgtgtg
     1201 gtggccgatg agcgcgacgc cttcgaggcg tccggcgcgg tcgcggcagg cgtggaacgt
     1261 gccgcggttc gaagggctga ccacgccgtt gtcgcggatg agtccgacca tgaagaccgt
     1321 gtggaggggg cgcacggcca gaaagtcgag cgcctcctgc cggtgctcct ctgccagtgg
     1381 ttgcggcgcg agaaccgccg gaggcgcttc cgcggcgggg cgtgagtgcg gcccgtctcc
     1441 cggaatctcg aacggtgtcg ttgaaaacat acgggcgttg ctcctacacg gagggcgagg
     1501 tcatgcaatc cagtagtctg ggtcgtgcgg ggttccaacg gctgcggctc gaactctgac
     1561 ctttgtcgtg ggctggcggc agccgtgccg gcaggttggg agatcacgcc cgcggcagaa
     1621 aggacattac gcccgcagcg ggtatcgcaa cgcacgtgcc accgaccggg cgcgaaagat
     1681 gaagggattt gccggggttg tcgtcggtcg tgaggctcgt cggtcatggc aaagtgactg
     1741 aatcagtcac tttgccatga cgacaattcg tctatctgac ggggtcgggg ttcggcgcgg
     1801 ccaaaggcgg cgggccgaag agggttccgg cgggtcacga cggggcgcgc ggcctccgcc
     1861 gccgcttcac tgcgggttga tgaagccgct gctccgggcc gtgtggtcga acttgtattc
     1921 gtagacgtag ccgggcaggc ggatgggctt cgtccattgc gcgtgcgcga cgattttgcc
     1981 gttctggttc tgcacttcga ggcgcatgtc gggcggcagg tcgttctcct tcgcgttggc
     2041 ccgcaactgc gtctcgaccc aggtggttgt ctggcccggc gggtaggcgg ccttgttcac
     2101 ggtctcttcc atgaagtcgc ggaagaggac tgcgctgtag gctacgggcg cgtactggta
     2161 ggcgcagtac gcggcggcgc cgaggacgag gaggacgacg agcaggttca ggcccgagcc
     2221 tccgcgctcg ccgcggttgc cctgaccgcg gcgagtcgtg tgcgagagtt ccatgtcggg
     2281 gttccttcta tggagtggtt cttacttcgg ggcgctagca gagcgccgcg cgaagcgact
     2341 cgcggacgac gctcgccgtg acctcccttc cgtccacgac gcgcgcgcgc ccgagccttt
     2401 ccagcagcac ccagcggacg cttccgccga cacttttttt gtccgcacgg agcgcgcgac
     2461 gaatcgcgtc cggcccgagg tcgtcggcgc gcggcaggcg gcccgccagt cgcaccgctt
     2521 cgcgcaaaga ttctaactcc gttgcgccga gcaagcctag ccttgtcgac agctcggcgg
     2581 cgaccagcat cccgtggccg accgcctcgc cgtggcggaa gcggcggtag cgcgtcagcg
     2641 cttcgagcgc gtgcccgacc gtgtggccga agttcaggat gctccgcgaa cgcgcgtccg
     2701 tgcgccccac ttcctcgcgc tcgtcgcccg ccacgatctt cgccttgaac gcgcactgcg
     2761 ccgcgatcag cgcggccaac tcatcttcgt cacgacccct cccgtcaacc tcatcgaccg
     2821 cgcccgcagc gactctcttt ccgacccgcg ccgctttcgt cccggcccgc gcgccctgcg
     2881 tcaagtattc gaccgtccgg cggaacagcg ccgcgtcgcc cgccgcgccc tgcttgatcg
     2941 cctcgcacca cccggcggtc agctcgcgcc gcggcaacgt ccgcagagtc tccgtgtcga
     3001 tcaacaccgc gcgcgggtgg tgaaacgcgc cgaccagatt cttgcccgcc gccgtgttga
     3061 cacccgtctt cccgccgacc gacgaatcga tctgcgcgag cagcgtcgtc ggcgcctgga
     3121 tgaaagcgac gccgcgcagg tacagcgccg ccgcgaaccc cgcgaggtcg ccgaccacgc
     3181 cgccgccgag cgccaccacc gcgtcgctcc tctcaagcct cgcctcggac atgaaccgca
     3241 acgcgcgctc cgccgtcttc aaactcttga agcgctcgcc ctcgcccatc atccactgcg
     3301 acacgtcgaa gcccgcgccg cgcaggctct cgacgacacg cccgccgtag agtccgaaca
     3361 cgcgcttgtt cgagacgacc gccacgcgcc gcgcgtgcgg cgacaggcgc tcgcgcgcga
     3421 cagcgcccac acgcccgagc gcccccgcgc cgatctctat cggatacccc gcgccccgct
     3481 tgaggcgcac gctcaccttc cgcatctctt tatcgtcaac catgcgacca ttcgagttcg
     3541 ccgtcgggac acgaaggaac gagaccgatc tctttattcc gccagtgcct tcgtgtcccg
     3601 gtggcgagga ttattcttca cactccttca cccaacaccg cgagcagttc gtcggccagc
     3661 aggtcgaagc tgagttcccc gccccaggcg agccgcgcca gctcttcgac cgtgtaacgc
     3721 gacgcggtgt tccacacgtc gatcaactcg tcgcccgccg cgcgcgaggc gtaccagcgc
     3781 cgcccgtgcc gcgcacgcag gtgctcgcgc gagctggccg cgaagagccg cgcacgcaag
     3841 cgcgtcgccg accgcgccgc cctctccgcg tccagcaggt gcgtggccgc gtcgtaacgg
     3901 aagccggtcg cgtcggcatg cgacgagagg taggcttcct tcaactgctc ggagcgcgcg
     3961 tcgcccgccg acgagagcgc cagccagtag cgcagcccgg cgcagtcgcg tcgcgcgtcg
     4021 tgcagttcga gcagcgccaa agtgcgcgtc acctcttccg cctcggtcgc gcgcatcgcg
     4081 ctctgcgcgc cgagccacgc cgcgtcgcgg agcaggcccg cgaacaacag cgcgtggcct
     4141 tcggcggtcg ccccgtcggg cgcgtagacg aactccgggt aacgcgccgc cgcctcgccc
     4201 gacgcccagc cgaaactttg cgcccgaccc gcctcgcgga acgtctcgcg cgcccactgc
     4261 gcgccgccgc ccctcgcgcc gaccacgagc cgcacgtcct tcggcggctc gacggcgaag
     4321 cacgccgacc gttccttctt cgacggtcgc ggctcggcgt ccaccttcac gttgcgctgc
     4381 cctccggggc gtacccccaa gcccgcgagc gtccacgcgt aggccgcgcc gaaccattca
     4441 gccgggaagt aaacgtctag gtgcgccgcg cgttcgaaga acaactcgtc ggcgtagtgc
     4501 acgccgcccg ccgcgccccg cggcgcgtga cgcgcggccc aacgcgagag gtgcgcggag
     4561 taagcgcgtg cggtgcgctc aaggaagatt tccgccgcgg cggtcaacgc ttcgagaccg
     4621 acgccggtca cgtcttcgtg cagtgcgcgc agatggtcga agccgagttc gcgcgccgcc
     4681 tcgttcaaag cttcgaggcg cgcggcgcgc aggtcttcgc aagggcgcac ggcgtcgaac
     4741 cagcgggccg cgagttcacg ccgacgaacg gcgtcgctct cgaaggcgac gagtcccggc
     4801 gcttccgacg ccgcgacctc cgcgccgccc caaccgattc gcccgcccgc ctcgcagcga
     4861 acgagttctt ccgtcacctc acgcgcgcgc acttcgagat atgccagtcg tgccgctccg
     4921 gcgagagcgc ggaggccggc gcgctcggtc tcgaactgcc ccggcgactc ttcataggcg
     4981 cggcgctgtt cgtcgatgga ttcgcgcgtc cagaggtctg cgtagcgctc ctcgacctgt
     5041 tcaaggtcgg ggagcgcgac gagacccgcg tgatgcctgt accgctcgcg ctcgacggcg
     5101 gagcggtagg cggcgtagtc tcgtcgatag tcgctcgggt tcatcttccg cggggcacgg
     5161 ctccggcccg gtagaacgag tcctgaattt gcagatgtgg ttgactgatt ttgattgtag
     5221 cacggacggc gggagcctga ttccgtctgc ccgacgggac ggcggcagcc ttccgcgata
     5281 cacgacttga gaggtcgcgc gagacgcacc acgtcgtcgc ttcaacgccc attgagccgg
     5341 ttagtgcctc gctgcggtct ggttggaact tgagctggta gagagtcgaa actcgaactt
     5401 ggagagactg gccgcggaag atttcgtcta aataaagaga acgggcgcgg caaacttggg
     5461 gaatgccacg cccgttctaa ttcgtggtta ctgtcctcac cccagcaacc actgagacgg
     5521 aggatccacc aaccgtctct cttgccccgc aatatacatc gcaatgctta tgccatgcaa
     5581 ggagaatttc aatcgtgttg aaaaacgtgg gttttgctgg ctttttttga agaaacggga
     5641 acgcctctgt gtgaactccg ggagtggcgt gtaactctta cactccaccg cgcgaaaatt
     5701 tttctcatcc cgccccgagg ttatcacgga taacagaggc caacgcggac gccaaagcct
     5761 ctatttcatc ctgccgctcg ccctcgatca tgacgcgcgc cagcggctcc gtccccgaat
     5821 aacgcagcag cagtcggcca cgcccgccga gcttcgcctc tgtctcgcgc gcgcaccgcg
     5881 cgatctctcc cacctcttcg aacggcaact tttcgcgcac gcgcacgttc gtcaagacct
     5941 gcgggtagcg ctcgaagccc gcggtcagct cgtgcagggc ggcgcccgtc tcgcgcatca
     6001 cgcgcaacaa ctggatggcc gtcagcatcc cgtcgcccgc aaggctgagg tgcgggaaga
     6061 tgatgtggcc cgactgctcg ccgcccaaca cggcgcccgt gcggagcagc tcttcgagca
     6121 cgtacttgtc gcccacgtcc gtgcgcagca gctcgatgcc gcgcgaccgc agcgcgagtt
     6181 caagcccgat gttgctcatc acggtggcga ccaccgtgcc accctcaagc tcgccgcgct
     6241 ccgacatgcg tttggccagc gcccacatcg tcgcgtcgcc gtccacgaac gtgccgcgcg
     6301 cgtccacgaa gagcgagcgg tccgcgtcgc cgtcgaaagc gacgccgatg tccgccttct
     6361 cttcggagac cctctgccgc aagccttcga tgtggagcga gccgcagtcg cggttgatgt
     6421 tgcgaccgtc gggcgcaacg tggagccgga cgacgcgcgc gccgaggcgt tcgaagagcg
     6481 cgggcgcgag ttcgaaggcc gcgccgttgg cgcaatcgac cacgacgccg aggccgtcga
     6541 gccgcaggcc ggcggcgatt gtcccgacga ggtagtcgag gtagcgcgtt cttaacgctc
     6601 gcgcgtgttg ttcgtcttgt gcgccagcgg aggcgctctc cgttcccgtc tcgacttcgc
     6661 cgagagcttc cgtcttcggt tcgcagtctt cgttcgagac ggggcgcgcg cccgccgtct
     6721 cggcatggat gtcggcctcg atgagccgct cggtcgcctc gtcgagcttg cggcccgtcg
     6781 gcgcgaaaat cttgatgccg ttatcctgat acggattgtg cgaggcgctg atgacgacgc
     6841 ccgcgtccgc cggcagcgtg cgcgcgagga aggccacgcc cggcgtcgtg atgacgcccg
     6901 ccgagacggc ctccgccgcc ccgcccgcga gcgcgccttc gaggaacgcg cgctcgatcc
     6961 agccgcccga ctcgcgcgtg tcgcgccccg cgacgacgcg cggcgcgcgc ccggcctgcg
     7021 ccgtcaggtg tctggcgagc gagcgccccg ccgcgcgcac cgtcgccgcg tcgagctagt
     7081 caacaccaac ccttcagagt ttaccctcga cagatgaaga gacttttcgg cacggacgga
     7141 atgcgcggcg aggcggggca gttcccgctg gatgcggcga cggtgcgcac ggcggggcgt
     7201 tcgctcgcgc ggc
//
LOCUS       DBNGOH010000102         4972 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2030805, whole genome shotgun sequence.
ACCESSION   DBNGOH010000102 DBNGOH010000000
VERSION     DBNGOH010000102.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4972)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4972)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4972
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2030805"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(228..1376)
                     /locus_tag="AC159P_01935"
     CDS             complement(228..1376)
                     /locus_tag="AC159P_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012811483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HIR3694241.1"
                     /translation="MSKSDEKAAGAKSPAGLEGVVAAESSIGDVDGINGVLIYQGLNI
                     HDLAEHATFEEVVYLLWHGRLPKREELEAQRRELAAEMTLPAEIVELVRRFPRDAEPM
                     DALRTAVSALAFYDGGARDLSREGALRTATKLTAQFPVVVAAIDRIRNGKEPVAPKPE
                     LNLAANFLYMLKGELPSEREARIFDVCLILHADHELNASTFTARVVAGTLADMYGAVT
                     GAIAALSGPLHGGANTNVMKMLLEIEDPARAEEWLKDALARKKKIMGFGHRVYKTEDP
                     RSVWLRKFSKEMGEAKGETRWNEILERLREVMFRDKKLYPNVDYYSGSAYYLMGIPLD
                     LFTPIFAVSRISGWTGHILEQYANNRLIRPRADYVGPRNVPYVPIDQR"
     gene            complement(1491..2309)
                     /locus_tag="AC159P_01940"
     CDS             complement(1491..2309)
                     /locus_tag="AC159P_01940"
                     /inference="COORDINATES: protein motif:HMM:NF014003.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM hydrolase/SAM-dependent halogenase family
                     protein"
                     /protein_id="HIR3694242.1"
                     /translation="MIVTLLTDFGTVDYFVGAMKGAVLSVNPQAQVVDITHEVPAHDV
                     EAGAFTLRAAFETFPAGTVHVAVVDPGVGSSRRALVARGGGHLFVGPDNGVLGHVYER
                     VGDPEVFQLTNTQFFREPVSTTFHGRDIFAPVAGALSRGVGPEALGTLVEDFVRLPLA
                     APTRDADGTVNGAIIHIDRFGNCITNLTPRDLPPATAVTEETNDSGALVIVVGDREVS
                     AFRRFFAQEGSASTEPFAIWGSTGLLELAVYRDSAARLLDTRRGQKIKAVNRKP"
     gene            complement(2395..3408)
                     /locus_tag="AC159P_01945"
     CDS             complement(2395..3408)
                     /locus_tag="AC159P_01945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694243.1"
                     /translation="MRTRTLYLAAILAVASTLAAPSAPFVLTASARAQQQQQYGALER
                     GYRTGYSDGYQSGWGDQLKGARADHRSKAEYRSADRAYIAAYGSLEDYRDGYRQGFEV
                     GYAAGFSRRGFESELPPGGISRRGVNDDDLTTADDGQSRGGVTGNATGTVNDGAASPG
                     VMRPIPSNTVLLVELENRLSTDVSQRGDRFEARVLEPVEYEGAIVGGRLTQVERSGKA
                     KGRSQLQLDFDRIRMPGGQWTDFSAQVIEVVDRGGEDNVGDVDEEGGVRGKSTTKDDI
                     AKVGAGAGIGAIIGAIAGGGKGAAIGAAIGGSVGTAGVLTQRGKDIRLERGQQLRIRT
                     SSR"
     gene            3738..4961
                     /locus_tag="AC159P_01950"
     CDS             3738..4961
                     /locus_tag="AC159P_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011345085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HIR3694244.1"
                     /translation="MRRVYMDNSATTPVDRRVVDAMLPFLTERFGNPSSVHFYGQEAR
                     AAVDRARREVAALINARPNEVVFLSGGTEANNLAVRGVAEMNAERGRHIITSSIEHSS
                     VRGICDALEERGWEVTRLPAYEEGVVRVEDVRAAVRPDTVLVTLMLANNEIGTVQPVA
                     EVGALVRELRAAGHKHLWLHTDAVQSLGRMAVDVEALGCDLLSMSAHKLHAPKGIGAL
                     YVRRSVRLAPQNIGGHQERERRGGTEAVSMIVAFGEAARLAREELDARITHMRALRDR
                     FEAGVAERVTDIVFNGDRGRRLPHISNISFRFIEGEGLLINLDMQGVAVSTGSACSSG
                     SLEPSPVIRALGRDDELARGSIRFSLGKDTTEEEIDYVLEVLPRAVENLRRLSPLYQK
                     ALAERSCETTPSYQF"
BASE COUNT          810 a         1615 c         1676 g          871 t
ORIGIN      
        1 gacaaacgaa tcgacccgga ctggccctcc ttcaaacgcg ggcgcgaccc cgtcatggaa
       61 tggattctca aacagtaggc ggaaggaaga agggaaaaag gaagaagggg aaaaggttaa
      121 aaggggaaaa ggcgaaaagg aaaacgagag acagcgtgcc tctcatcctc cctcttcgcc
      181 ttttcccctt ttaacctttt ccccttttcg cctcttcgcc tccggtttta tcgctggtca
      241 atcggcacgt acggcacatt gcgggggccg acgtagtctg cgcgcggtcg aatgaggcgg
      301 ttgttggcgt actgttcgag gatgtggccc gtccagccgg agatgcggct gacggcgaag
      361 atgggcgtga agaggtcgag cgggatgccc atcaggtagt aggcggagcc ggagtagtaa
      421 tcgacgttgg ggtagagctt cttgtcgcgg aacatcacct cgcggaggcg ttcgagaatc
      481 tcgttccagc gcgtctcgcc tttggcctcg cccatctctt tggagaattt gcggagccac
      541 accgagcgcg ggtcttcggt cttgtagacg cggtggccga agcccatgat cttcttcttc
      601 cgcgcgagcg cgtccttcag ccactcctcg gcccgcgcgg ggtcttcgat ttcgagcagc
      661 atcttcatga cgttcgtgtt cgcgccgccg tgcagcggcc ccgacaaagc ggcgatggcg
      721 cccgtcaccg cgccgtacat gtcggcgagc gtgccggcga cgacgcgcgc cgtgaaggtc
      781 gaggcgttca gctcgtgatc cgcgtgcagg atgaggcaca cgtcgaagat gcgcgcctcg
      841 cgctccgagg gcagctcgcc cttgagcatg tagaggaagt tggcggcgag gttcagctcc
      901 ggtttcggcg cgaccggttc cttgccgttg cggatgcggt cgatggcggc gacgacgacc
      961 gggaactgcg ccgtcagctt cgtcgccgtg cgcagcgcgc cctcgcgcga gaggtcgcgg
     1021 gcgcccccgt cgtagaaggc gagcgcggag accgccgtgc gcagtgcgtc catcggctcg
     1081 gcgtcgcgcg ggaagcggcg gacgagttcg acgatctcgg cgggcaaggt catctcggcg
     1141 gccagctcgc ggcgctgggc ctcaagctcc tcgcgtttgg ggagccgccc gtgccagagc
     1201 aggtagacga cctcttcgaa ggtcgcgtgc tcggcgaggt cgtggatgtt gaggccctga
     1261 tagatgagga cgccgttgat gccgtccacg tcgccgatgg aggactcggc ggccaccacc
     1321 ccctccagcc ccgccggact cttcgccccc gcggcctttt cgtcggattt actcatggtg
     1381 ccgtccttaa aagttagagt ggattgaaaa taacaaaaaa gccgtgaacc gtaaaccggt
     1441 aagaaccgtg aaccgtgaca agtaaaaacc agttcttcac ttatcacggt tcacggttta
     1501 cggttcacgg cttttatctt ctggccgcgg cgcgtgtcga gcaggcgggc ggcggagtcg
     1561 cgatagacgg cgagttcgag gaggccggtg ctgccccaga tggcgaaagg ctccgtcgag
     1621 gcgcttccct cttgggcgaa gaagcggcgg aaggcggaga cctcccggtc gccgacgacg
     1681 atgacgagag cgccgctgtc gttcgtctct tccgtcactg ccgttgcagg ggggaggtcg
     1741 cgcggcgtga ggttggtgat gcagttgccg aagcggtcga tgtggatgat ggcgccgttg
     1801 acagtgccgt ccgcgtcgcg cgtcggcgcg gcgagcggga ggcgcacgaa gtcttcgacc
     1861 agagtgccga gcgcctccgg cccgacgccg cgcgagaggg cgcccgccac gggcgcgaag
     1921 atgtcgcgcc cgtggaaggt cgtgctgacc ggctcgcgga agaactgcgt gttggtgagc
     1981 tggaagacct cggggtcgcc gacgcgctcg tagacgtggc cgagcacgcc gttgtctggg
     2041 ccgacgaaga ggtggccgcc cccgcgcgcg acgagggcgc gacgcgaaga gccgacgccg
     2101 ggatcgacga ccgcgacgtg caccgtcccc gcggggaacg tctcgaacgc cgcgcgcaga
     2161 gtgaacgcgc ccgcctccac gtcgtgcgcg ggcacctcgt gcgtgatgtc tacgacctgt
     2221 gcttgcgggt tgacggagag gacggcgccc ttcatcgcgc cgacgaagta atcgacggtg
     2281 ccgaaatcgg tcaggagagt gacgatcatg aaaagataga ggtcagaggt cggaggtcgg
     2341 aggtcagtgg aaagcaagtg tctttcactg acctccgacc tctgacctct ggatttatct
     2401 gctgctcgtg cggatgcgga gttgctggcc gcgttcgagg cggatgtctt tgccgcgctg
     2461 cgtgaggacg cccgcggtgc cgaccgagcc gccgatggcc gcgccgatgg ccgcgccctt
     2521 gccgccgccc gcaatcgcgc cgatgatcgc gccgatgccg gcgcccgcgc cgaccttcgc
     2581 gatgtcgtcc ttcgtcgtgc tcttgccgcg cacgccgccc tcctcgtcaa cgtcgccgac
     2641 gttgtcttcg ccgccgcggt caaccacttc gatcacctgc gcgctgaagt ctgtccactg
     2701 gccgcccggc atcctgatcc ggtcgaagtc gagctggagc tgcgaacggc ccttcgcctt
     2761 gcccgagcgc tcgacctgcg tgagccgccc gccgacaatc gcgccctcgt actcgaccgg
     2821 ctcaagcacg cgcgcctcga agcggtcgcc gcgctggctc acgtccgtcg aaagacggtt
     2881 ctcaagctcg acgagcagca ccgtgttcga agggatgggc cgcatcacgc ccggcgacgc
     2941 cgcgccgtcg ttgacggtgc cggtcgcgtt gcccgtcacc ccgccgcgcg actggccgtc
     3001 gtccgcggtc gtcaagtcgt cgtcgttcac gccgcgccgc gagatgccgc ccggcgggag
     3061 ttcggactcg aagccgcggc ggctgaagcc cgcggcgtag ccgacctcga agccctgccg
     3121 gtagccgtcg cggtagtctt cgagcgagcc gtaggcggcg atgtaggcgc ggtcagcgct
     3181 ccggtactcg gccttcgagc ggtggtctgc ccgcgcgccc ttgagctgat cgccccagcc
     3241 cgactggtag ccgtccgagt agcccgtgcg gtagccgcgt tcgagcgcgc cgtattgctg
     3301 ctgctgctgc gcgcgcgcgg aggccgtcag gacgaacggc gccgacggcg cggcgagagt
     3361 cgatgcgacc gcgaggattg ctgccaggta aagcgtgcgt gttctcatat ctttgatctc
     3421 cgggggccgg gcctgtgagc cttcgcggga gagattatag caggccgttt ctcaaccgat
     3481 taaccgcctt gacgctcgat ggcgcgcgac ttagactcga agcgcggggg cgttcaagac
     3541 actttccggc gggaatattt tttcccgccg cctttgatgc ggcgcacgaa cccgcaggtt
     3601 cgacgttttt ttagctttta gcaattagct gttagctttt ggcttaatgc ttgtaaggtt
     3661 gatgggagcg gcgctgtcat caatcgtcaa tcgctaatcc gacagctaac agctaacagc
     3721 taacagctaa cagctatatg cgcagagtct acatggacaa cagcgcgacg acgccggttg
     3781 accggcgcgt cgtggacgcg atgctgccgt ttttgaccga gcggttcggc aacccttcga
     3841 gcgtccactt ctacgggcag gaggcgcgcg cggcggttga ccgcgcgcgg cgcgaagtcg
     3901 cggccttgat caacgcgcgc ccgaacgaag tcgtcttcct ctccggcggc accgaggcca
     3961 acaacctcgc cgtgcgcggc gtcgccgaga tgaacgcaga gcgcgggcgc cacatcatca
     4021 cctcgtccat cgagcactct tcggtgcgcg gcatctgcga cgcgctcgaa gagcgcggct
     4081 gggaggtgac gcggctgccc gcttacgagg agggcgtcgt ccgcgtcgag gacgtgcgcg
     4141 ccgccgtcag gcccgacacc gtgctcgtca cgctgatgct cgccaacaac gagatcggca
     4201 ccgtccagcc cgtcgcagag gtcggcgcgc tcgtccgcga gctgcgcgcc gccgggcaca
     4261 aacatctgtg gctccacacc gacgccgtcc agtcgctcgg gcgcatggcg gttgacgtgg
     4321 aagctctcgg ctgcgacctg ctctcgatgt cggcgcacaa gctgcacgcg ccgaagggca
     4381 tcggcgcgct gtacgtgcgc cgcagcgtgc ggctcgcgcc gcagaacatc ggcggccatc
     4441 aggagcgcga gcggcgcggc ggcaccgagg ccgtctcgat gatcgtcgcc ttcggcgagg
     4501 ccgcgcgcct cgcgcgcgaa gaactcgacg cgcgcatcac acacatgcgc gccttgcgcg
     4561 accgcttcga agcgggggtg gccgagcggg tcacggacat cgtcttcaac ggcgaccgcg
     4621 ggcgcaggct gccgcacatc tcgaacatct ccttccgctt catcgaaggc gaagggctgc
     4681 tcatcaacct cgacatgcag ggcgtcgccg tctccacggg gtcggcctgc tcgtccggct
     4741 cgctcgaacc ctcgcccgtc atccgcgcgc tcgggcgcga cgacgagctg gcgcgcggct
     4801 ccatccgctt cagtctcggc aaggacacga ccgaagagga gattgactat gtcctcgaag
     4861 tcctgccccg cgccgtcgaa aacctgcgcc gcctctcgcc gctctaccag aaagctctcg
     4921 ctgaacgctc ctgcgaaaca actccgagtt accagttttg agttttcagt tt
//
LOCUS       DBNGOH010000103         1787 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4047017, whole genome shotgun sequence.
ACCESSION   DBNGOH010000103 DBNGOH010000000
VERSION     DBNGOH010000103.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1787)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1787)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1787
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4047017"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1787)
                     /locus_tag="AC159P_01955"
     CDS             complement(<1..>1787)
                     /locus_tag="AC159P_01955"
                     /inference="COORDINATES: protein motif:HMM:NF012618.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694245.1"
                     /translation="DEAERQAGIARTNEQRANDALADAREQTEKALAANESANNLIYT
                     SDINIIEDSEEEGDTERSRQILENHLPPSLLPPSLKAGASETSPVSVWADLSGLSGAD
                     SPGDRKRAGAPGGAAVAPEEDRRDFSWFYFWRLNHRDARALEAGQGAVSSVGYSNDGK
                     VIAAAYYTGLVVLWDDSGKEIKRRQQDASVISAALSPDGKSVVVVGDDNTVHLWNTES
                     GDPPALLGSFKDPAEFVAYSHDGKLIAAATRKGELKLWLAATRAAPPKVPAGSDASIN
                     SIAFTPDEDVLVVAGSRGLRVLDLEEGREGEEKFVKEIVVTSLAFDSDTTIALGDQQG
                     TVTLYEAGNVDKLGSFNAHRGPITSLSFSPDGKTLATASGENNAKLWDVATQQKLVTL
                     NNPRGSVSRVAFSPDGKTLATGGEDGAVKLWDATLLRGYVEPEMPKDIRPVAVAAAAF
                     SPDRRLLAALSETGNIIVWDAASYGTPNRLLEDVDDRGFSAFAFSPNGGKLAAANSAG
                     KVLLFDTTSWADEPLDLQSFDDVRITSLAFSSDGNLLAIGSGISFTLWDTRARREVEV
                     ETGGRGRSPLALSRAGDLLAVTTEDQTVAL"
BASE COUNT          259 a          592 c          594 g          342 t
ORIGIN      
        1 agcgcgacgg tctggtcttc ggtcgtgacc gcgagcaggt cgcccgcgcg cgaaagggcg
       61 aggggcgacc tcccccgccc gcccgtctcc acttccactt cccgccgcgc gcgggtgtcc
      121 cagagcgtga acgaaatgcc actgccgatg gcgagcaagt tgccgtcgga cgaaaaggcg
      181 agggacgtga ttcgcacgtc gtcgaaactc tgcaagtcca gcggctcgtc cgcccacgag
      241 gtcgtgtcga acagaagcac cttgcccgcg ctgttggcag ccgcgagctt cccgccattc
      301 ggggagaagg cgaaggcgga gaagcctctg tcgtcaacgt cctccagcaa cctgttcggc
      361 gtgccgtagg aggcggcgtc ccacacgatg atgttcccgg tctcgctgag cgcggcgagc
      421 agcctgcggt ccggcgaaaa ggcggcagcg gcgacggcga cgggccgtat gtctttcggc
      481 atctcaggct cgacgtagcc tctgagcagc gtcgcgtccc agagcttcac ggccccgtcc
      541 tcgccgccgg tggcgagcgt cttcccgtcg ggcgagaagg cgacgcgcga gacggagcca
      601 cgcgggttgt tgagcgtgac cagcttctgc tgcgtcgcca cgtcccacag cttcgcgttg
      661 ttctccccgc tggcggtggc cagcgtcttc ccgtcgggcg agaaggacag agacgtgatc
      721 ggccccctgt gcgcgttgaa gctgcccagt ttatctacgt tgcccgcctc gtacagtgtc
      781 acggtgcctt gttgatcacc cagtgcgatt gttgtgtcgc tgtcaaaggc cagcgatgtg
      841 acgacgatct cctttacgaa tttctcttcg ccttctctgc cttcctccaa gtccaagacc
      901 cttaaaccgc gagaaccggc gacgaccagc acgtcttcgt cgggcgtaaa ggcgattgag
      961 ttgattgacg cgtcggagcc ggcgggaacc ttaggcggcg cggcccttgt cgcggcgagc
     1021 cacagcttca actctccctt gcgggtagcc gcggcgatca gtttgccgtc gtgcgaatag
     1081 gcgacaaact ccgcggggtc tttgaagctg ccgagcaggg cgggcgggtc gccggattcg
     1141 gtgttccaca agtggacggt gttatcgtcg ccgacgacga cgaccgactt gccgtcgggc
     1201 gaaagggccg ccgagatcac ggacgcgtcc tgctgtcttc tcttgatctc cttgcccgag
     1261 tcatcccaca gcaccacgag gccggtgtag taagcggcgg cgatcacctt tccgtcgttc
     1321 gaatagccga ccgagctgac cgcgccctgc ccggcttcga gcgcgcgcgc gtccctgtgg
     1381 ttgagccgcc agaagtagaa ccaactgaag tcgcgtctgt cctcttcggg cgcgacggcg
     1441 gcgccgccgg gcgcgcctgc tctcttccgg tcgccgggcg aatccgcgcc ggagaggccg
     1501 gacaggtcgg cccacacgct gacgggggag gtctcggacg cccccgcctt caggctcggg
     1561 ggcagcaggc tcgggggcag gtggttttcg agtatctgcc ggctgcgctc ggtgtcgccc
     1621 tcctcttcgc tgtcctcgat gatgttgatg tccgaggtgt agatcaggtt attggcgctc
     1681 tcgttcgctg cgagcgcctt ctcggtctgc tcgcgggcgt cggccagcgc gtcgttggcc
     1741 ctctgttcgt tcgtgcgggc gatgcccgcc tgccgctcgg cttcgtc
//
LOCUS       DBNGOH010000104         5324 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2580816, whole genome shotgun sequence.
ACCESSION   DBNGOH010000104 DBNGOH010000000
VERSION     DBNGOH010000104.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5324)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5324)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5324
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2580816"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1963
                     /locus_tag="AC159P_01960"
     CDS             <1..1963
                     /locus_tag="AC159P_01960"
                     /inference="COORDINATES: protein motif:HMM:NF025006.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HIR3694246.1"
                     /translation="PRRLFALLSLLACACLAAYALAPPRVARAQDEDEDKAQKGAAPD
                     EHKEEPKSVVYGRAVYDGTSRPVRRARVTLASRAERTRQYVGLTDANGEFRIGGVTPG
                     HYVAYVIAGGVISPVSFIPLAELSGGAPDLHRPELRRFFDEVEVDGKGDKQLTVRARR
                     GAAIGGKVTYANGDPAINVRVHIMRRSESRLSKFMPGSAPSSLAGLTTDDRGMFRVAG
                     LPPGEYVVGVSELADHADDNDSRPGTPEFVEGNAFYALMSDPLLTTYYPSTPRAADAT
                     PIKVEAGDERADVDITVAERELRSVGGVVRGRRDRRPVANARVAIARKSGGEGGPDAA
                     ATVFGEMTQTSVRTDAEGRWRLKDLPDGEYTLHAAPPEEPDEAAVALYEKMNPNAAPN
                     SNLSHARPQRKKRYASGRRDVKVSADIDDLSLELSDGARVAGRVTFEGGKPPEYSTLF
                     LTPSEPRPSEFAMEAVPGATVFNGVFQVEGLRPGKYYVHPNTYGVAEGGNEADDDFYV
                     KSISWNGRDLMHEPLEVGEGANVEDVRIVYARGPARLFVRVTTQADKEPARRALVFLV
                     PANTQGWSLYGQNPRCHTDDEGTCEVAGAPGDYKVVALAPQLVVSDTTDFEAEITLRA
                     ANAPRVTLRAAETKDLDLVLPGGSREKQK"
     gene            2355..2771
                     /locus_tag="AC159P_01965"
     CDS             2355..2771
                     /locus_tag="AC159P_01965"
                     /inference="COORDINATES: protein motif:HMM:NF018894.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HIR3694247.1"
                     /translation="MPELLRDIASRLRAFVGNRRYATRYPTRLPVAVSLFDARPRAYP
                     AALEGHTRDLSASGLAVVLPAIRIGERYLTGDTHVLRITLRLPAAAIQIHGVPTRYER
                     LGEDDADTGYLVGVRIKEMSDKDRALFEEHLRALKT"
     gene            complement(2890..3672)
                     /locus_tag="AC159P_01970"
     CDS             complement(2890..3672)
                     /locus_tag="AC159P_01970"
                     /inference="COORDINATES: protein motif:HMM:NF018190.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding family 9-like protein"
                     /protein_id="HIR3694248.1"
                     /translation="MTAWEEGMMRAPLVRTRLRFIVAASVVASSTTAVRPVFVAPDFE
                     MTERAEEIAEAVRARGKLGVTDFDHGEWGRARPVRVTRYWSGAEAPQGRHAEARVVWD
                     AEALSVRFVYRQEEPAVVAEAPRVDRKTLGLWERDVCEVFVAPDARRPAKYLEFEAAP
                     TGEWLDLAIVWRADERETDWEFRSGMQTAARVAGEEVTILMRVPWAAFGRTPRAGERW
                     RANFFRCAGRDPDRGYMAWRPTRTPEPSFHVPQAFGELEFKK"
     gene            complement(3839..4630)
                     /locus_tag="AC159P_01975"
     CDS             complement(3839..4630)
                     /locus_tag="AC159P_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR3694249.1"
                     /translation="MDLGLSGRVALVAAASGGIGYASALELAREGARVFICSRDEGRV
                     AKAAERMRAETGAEVVGVRADVTRDEDAEGFVRAALDAAGRVDVLVTNAGGSPGSTFD
                     STDLEMYRSAFELNALSAIRLARLSVPHMRERGWGRVVNVTSISVKQPIEGLLLSNTV
                     RAGLTGWAKTVSNEVAADGVTVNNVAPGWTLTARQEEHAETRGRQLGKTKEEMVEGWA
                     GEVAMRRMARPEEIAAAVAFLASERASYITGVTLVVDGGWVKGLY"
     gene            complement(4750..5298)
                     /locus_tag="AC159P_01980"
     CDS             complement(4750..5298)
                     /locus_tag="AC159P_01980"
                     /inference="COORDINATES: protein motif:HMM:NF037785.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLOG cluster 4 domain-containing protein"
                     /protein_id="HIR3694250.1"
                     /translation="MKVKVGVMGSAGDTLAEGVAEGLREKAEALGRELAARGCVVLTG
                     GTTGLPHLVGGAAHRAGAWHVGVSPAQDEREHVGRYGLPIDGTDVLIYTGFGLKGRNV
                     VLVRSCDVVLVVRGAMGALNEFTIAHDEGRVVGCLTGTGGAADEIEGLMKALPKKTAA
                     LVFFDDDPAALLDECLGALREL"
BASE COUNT          877 a         2133 c         1560 g          754 t
ORIGIN      
        1 cccccgccgc ctcttcgccc tcctctcact gctcgcgtgc gcgtgcctcg ccgcctacgc
       61 cctcgccccg ccgcgagtcg cgcgcgcgca ggacgaggac gaggacaaag cgcagaaggg
      121 cgcggcgccg gacgagcaca aggaagaacc caagtccgtc gtctacgggc gggcggtcta
      181 cgacggcacg agccgccccg tgcgccgcgc gcgcgtcacg ctcgcgagcc gcgccgagcg
      241 cacgcgccag tacgtcgggc tgacggacgc gaacggcgag ttccgcatcg gcggcgtcac
      301 gcccggtcac tacgtcgctt acgtcatcgc cggcggcgtc atctcgcccg tcagcttcat
      361 accgctcgcc gagctgagcg ggggggcgcc cgacctccac aggccggagc tgcgccggtt
      421 cttcgacgag gtcgaggtgg acggcaaggg cgacaagcag ttgacggtgc gcgcgcggcg
      481 cggcgcggcc atcggcggca aggtcacgta cgccaacggc gacccggcca tcaacgtccg
      541 cgtccacatc atgcgccgca gcgagagccg cctctcgaag ttcatgcccg gctccgcgcc
      601 ctccagcctc gccggcctga ccacggacga ccgcggcatg ttccgcgtcg cgggcctccc
      661 gcccggcgaa tacgtcgtcg gcgtctcgga gctggccgac cacgccgacg acaacgactc
      721 gcgccccggc acgcccgagt tcgtcgaggg caacgcgttc tacgccctga tgagcgaccc
      781 gctgctcacg acctactacc cctcgacccc gcgcgccgcc gacgcgaccc ccatcaaggt
      841 cgaggcgggc gacgagcgag ccgacgttga catcacggtc gccgagcgcg agctgcgctc
      901 ggtcggcggc gtcgtgcgcg gtcgccgcga ccgccgtccc gtggcgaacg cgagggtggc
      961 catcgcccgc aagagcggag gcgaaggcgg cccggacgcc gccgccaccg tcttcggcga
     1021 gatgacgcag acgagcgtcc ggacggacgc cgaagggcgc tggcggctga aagacctccc
     1081 cgacggcgag tacacgctgc acgccgcgcc gcccgaagag cctgacgagg ccgccgtggc
     1141 cctgtacgag aaaatgaacc cgaacgcggc gccgaactcg aacctgagcc acgcgcgccc
     1201 gcagcggaag aagcgctacg cgtccggtcg gcgcgacgtg aaggtctccg ccgacatcga
     1261 cgatctgtcg ctcgaactga gcgacggcgc gcgcgtcgcg ggccgcgtca ccttcgaggg
     1321 cggcaagccg cccgagtaca gcaccctctt cctgacgccg agcgagccgc gtccgagcga
     1381 gttcgcgatg gaggcggtgc cgggcgcgac cgtcttcaac ggcgtctttc aggtcgaagg
     1441 actcaggccc ggcaagtact acgtgcaccc gaacacctac ggggtggctg agggcggcaa
     1501 cgaagcggac gacgatttct acgtcaagtc gatcagttgg aacgggcgcg acctcatgca
     1561 cgagccgctc gaagtcggcg agggggcgaa cgtcgaggac gtgcgcatcg tctacgcgcg
     1621 cggcccggcg cggctgttcg tgcgcgtcac cacgcaagcc gacaaggagc ctgcccgccg
     1681 cgcgctcgtc ttcctcgtcc ccgcgaacac gcagggctgg tcgctctacg ggcagaaccc
     1741 ccgatgccac accgacgacg aaggcacctg cgaggtcgcc ggcgcccccg gcgactacaa
     1801 agtggtcgcg ctcgcgccgc aactggtcgt ctccgacacc accgacttcg aggccgagat
     1861 caccctccgc gccgccaacg ccccgcgcgt caccctccgc gccgccgaga ccaaagacct
     1921 cgacctcgtt ctgcccggag ggagccggga aaagcaaaag taaaaaggcg atagcgtccg
     1981 cggtaagacg gatgcggagc acggcgcctg tcatcatcaa ctcgacgatg gcgagcgccg
     2041 tgctccgcgt tgacgacggg aggccgccgc gcttcgcatc aagtcgatgt tgcgaagcgc
     2101 ggcagcctcc cggcatccct ttcgcctttc ccctttttac ttttgccttt ctctgcggct
     2161 agaatgaagg tcggcggcgg ggactcggtt ccacgtcgcg cgccgcgtct gccccgacag
     2221 ttcctcttca aaatcggctc cgctgttttc ggtcgcgcgc ccgcgcgccg aaagcgccgc
     2281 tctccttggt gcgccgcggg tcgcgcccgc cggccaggtc tggccggggc cgcgacgcga
     2341 aacgacgagt ggccatgccg gaactgcttc gagacatcgc cagccgcctc cgcgcgttcg
     2401 tcggcaaccg ccgctacgcg acgcgctacc cgacgcgtct cccggtcgcc gtctcgctct
     2461 tcgacgcgcg gccccgcgcc taccccgccg cgctcgaagg ccacacgcgc gacctgagcg
     2521 ccagcggcct cgccgtcgtc ctgcccgcca tccgcatcgg cgagcgctac ctcacaggcg
     2581 acacgcacgt cctgcgcatc acgctccgcc tccccgccgc cgccatccag attcacggcg
     2641 tccccacgcg ctacgagcgc ctcggcgaag acgacgcgga caccggctat ctcgtcggcg
     2701 tccgcatcaa agagatgagc gacaaagacc gcgccctctt cgaagagcac ctgcgagcac
     2761 taaaaacata ggggaaaagg ttaaaagggg aagaggggaa aagggaagaa gcgacgcgca
     2821 cggcaagctc ttccttttcc cctcttcccc ttttaacctt ttcccctttt aacctttctt
     2881 cctctttccc tattttttga actccaactc gccgaacgcc tgcgggacgt ggaagctcgg
     2941 ctcgggcgtg cgcgtggggc gccaggccat gtagccgcgg tcggggtcgc ggcccgcgca
     3001 gcgaaagaag ttggcgcgcc agcgctcgcc cgcgcgcggc gtccgaccga acgccgccca
     3061 cggcacgcgc atcaagatgg tcacctcctc gcccgcgacg cgcgccgccg tctgcatgcc
     3121 gctccggaac tcccagtcgg tctcgcgctc gtcggcccgc cacacaatcg ccagatcgag
     3181 ccactcgccc gtgggcgcgg cctcgaactc aagatacttc gccggcctcc gcgcgtccgg
     3241 cgcgacgaag acctcgcaca cgtcgcgctc ccacaacccg agcgttttgc ggtcaacgcg
     3301 cggcgcctcg gccacgaccg ccggctcctc ctgccggtag acgaaacgca cgctcaaagc
     3361 ctccgcgtcc cacacgacgc gcgcctccgc gtgccgcccc tgcggcgcct ccgcgcccga
     3421 ccagtagcgc gtcacgcgca cgggccgcgc gcggccccac tccccgtgat cgaagtccgt
     3481 gacgcccaac ttcccgcgcg cgcgcacagc ctcggcgatc tcttcggccc tctcagtcat
     3541 ctcgaaatcc ggcgcgacga aaacaggacg caccgccgtc gtgctcgaag cgacgacgga
     3601 tgccgcgacg atgaagcgga gacgggttct taccagcggc gccctcatca tcccttcctc
     3661 ccatgccgtc attcgtcaca cgtctctcat gcttcgccct tcgcccgcta cattttggat
     3721 tgtagagttt ggattttgga ttagcgtgct tctcaagatt gaagcaggct tttagacgct
     3781 caactcccgt caaccgaaca acccaacacc caaaacctac cacccgacac ccattccttc
     3841 agtacaaccc tttcacccac ccgccgtcaa cgacgagcgt gacgcccgtg atgtaagacg
     3901 cgcgctccga ggcgaggaag gcgaccgccg ccgcgatctc ctcgggccgc gccatgcgcc
     3961 gcatcgccac ctcgccggcc cagccctcga ccatctcctc cttcgtcttg ccgagctgcc
     4021 gcccgcgcgt ctccgcgtgc tcctcctgcc gcgccgtcag cgtccacccc ggcgcgacgt
     4081 tgttcaccgt caccccgtcc gccgccacct cgttcgacac ggtcttcgcc caccccgtca
     4141 accccgcgcg cacggtgttc gacagcagca gcccctctat cggctgcttc accgagatgg
     4201 atgtgacgtt caccacgcgc ccccaaccgc gctcgcgcat gtgcggcacc gagagccgcg
     4261 ccagcctgat cgcggagagc gcgttcagct cgaacgcgct ccggtacatc tcaaggtcgg
     4321 tcgaatcgaa cgtcgaaccc ggcgacccgc ccgcgttcgt caccagcacg tccacgcgtc
     4381 ccgccgcgtc cagcgccgcc cgcacgaacc cttcggcgtc ctcgtcccgc gtcacgtccg
     4441 cccgcacgcc caccacctcc gcgcccgtct ccgcccgcat cctctccgcg gccttcgcca
     4501 cgcgcccctc gtcgcgcgag cagatgaaga cccgcgcgcc ctcgcgcgcc agctccaacg
     4561 ccgacgcgta accgatcccc ccgctcgccg ccgccaccag agccacgcgc ccgctcaatc
     4621 ccaagtccat ccgtcacgct ccccaaaagg attaactgta ggggcagggc ttgtccctgc
     4681 ccgttcgatg ttcgccaccg tcaacgtcga attatcggca agacgcgggc cgaggacgag
     4741 cctctgcctc tacaattcac gcaaagcacc aagacactcg tcgagcagcg ccgccggatc
     4801 atcatcgaag aagaccagcg ctgcggtctt cttcggcaac gccttcatca gcccttcgat
     4861 ctcgtccgcc gcgccgcccg tccccgtcag gcagccgacg acgcgcccct cgtcgtgcgc
     4921 gatggtgaac tcgttcagag cacccatcgc cccgcggacg acgagcacca catcgcatga
     4981 gcgcaccagc accacgttgc gccccttcaa accgaacccc gtataaatca gcacgtccgt
     5041 cccgtcaatc ggcagcccgt aacgccccac gtgctcccgc tcgtcctgcg cgggcgacac
     5101 ccccacgtgc cacgcccccg ccctgtgcgc cgcgcccccg acaaggtgcg gcagccccgt
     5161 cgtccccccc gtcaacacca cgcacccccg cgccgccagc tcacgcccca acgcctccgc
     5221 cttctcgcgc aagccttcgg ccacgccctc cgccagcgtg tcgcccgccg aacccatcac
     5281 gcccacctta actttcatac ggaacccaag agccgtgcac cctg
//
LOCUS       DBNGOH010000105         3991 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1114938, whole genome shotgun sequence.
ACCESSION   DBNGOH010000105 DBNGOH010000000
VERSION     DBNGOH010000105.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3991)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3991)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3991
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1114938"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(190..537)
                     /locus_tag="AC159P_01985"
     CDS             complement(190..537)
                     /locus_tag="AC159P_01985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HIR3694251.1"
                     /translation="MNGDLGRQKKHLWRRRAQRMDVPLPAFWGLAEDDCQEVGIAQGL
                     SRGGCFVEALVDELRAETICLRVLLPTGRWLKLRAEAVYYASGRGIGASFTGISDEDA
                     AMIDLVTEHYGAE"
     gene            complement(586..867)
                     /locus_tag="AC159P_01990"
     CDS             complement(586..867)
                     /locus_tag="AC159P_01990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694252.1"
                     /translation="MGWPLPDLRLGREDELLVMALSSAALLLHGLGQLARFVADRGKA
                     DARSSHLSRTYPRSYRNTRRWRLRGLSAAATLLLGAALLLVTLSGYRGR"
     gene            complement(1023..1688)
                     /locus_tag="AC159P_01995"
     CDS             complement(1023..1688)
                     /locus_tag="AC159P_01995"
                     /inference="COORDINATES: protein motif:HMM:NF025887.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR3694253.1"
                     /translation="MSTQPAGGGTGTSDNAVAQEIIRLERQRAEAYVRRDIPTLERLL
                     PEHFTLTRAAGSFGKGELLLLIESGLLTFESLIRRVEAVKVYPGAAVAFGQDAVKARY
                     KDEDISGLYRFTNTYVCDGGQWAVVATHSSLIPQLKQINAIVAGLAAVYAANEISAAG
                     CPVCKVRLDHTSGCPVRKAWALLDEGTRAALKERVLASSGDFIALGNVKWPELLDKFG
                     TEL"
     gene            complement(1708..1911)
                     /locus_tag="AC159P_02000"
     CDS             complement(1708..1911)
                     /locus_tag="AC159P_02000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694254.1"
                     /translation="MSDDTPRKADQPAYRKSQPRLRLVKSPEAPPQPAPDTGLRAVLD
                     DMNHRYRVQRERAGRDGGGKDAA"
     gene            complement(1988..2332)
                     /locus_tag="AC159P_02005"
     CDS             complement(1988..2332)
                     /locus_tag="AC159P_02005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694255.1"
                     /translation="MHLLQLLSALAAAAAALITLAVHFGAHPDLLRATILLCLVVVVW
                     QGVAFVLGRGTQGRRGQESDGGSQALPARQGAAPPALNAADTEDLVRPPSVTENTTAL
                     LDPVPAKKGGER"
     gene            complement(2491..2640)
                     /locus_tag="AC159P_02010"
     CDS             complement(2491..2640)
                     /locus_tag="AC159P_02010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694256.1"
                     /translation="MRVIDMHSARGAALAAGYGVARVPAVVVAGRLDEDGGPDDDPRE
                     KGREE"
     gene            complement(2766..3005)
                     /locus_tag="AC159P_02015"
     CDS             complement(2766..3005)
                     /locus_tag="AC159P_02015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694257.1"
                     /translation="MGLGTWLRSRRRRAELERFRRQFAECGFNLDRWTDAEVEAAISR
                     APEAAGPACLTADGARHFLGVLMEVAEDDTDKPAS"
     gene            complement(3054..3461)
                     /locus_tag="AC159P_02020"
     CDS             complement(3054..3461)
                     /locus_tag="AC159P_02020"
                     /inference="COORDINATES: protein motif:HMM:NF025887.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR3694258.1"
                     /translation="MSTQAAAAGMGASDQAVTDEITRLELARAEAYLRRDVPALGRLL
                     PEHFTFNRSIGSFGKGELLALIESGELTFESLERRVESVKVYLATAVAFGKDEVKVRY
                     RGEDIGGRYRFTNTYVWDGERWVIVVTHSSRIP"
     gene            complement(3458..3871)
                     /locus_tag="AC159P_02025"
     CDS             complement(3458..3871)
                     /locus_tag="AC159P_02025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694259.1"
                     /translation="MSDAPTCEQCGSAMTADRLGGKAVGWHCAACRAKQTAGVMRRIG
                     RAASKAARLPKSQSPWCARCSRRVEESAVTPDPRRAGKAVVEYRCHGESARQEMPASL
                     LAGGLAAYTVFNEYSSGLMLGAVAPAAGKRKGGKR"
BASE COUNT          568 a         1513 c         1245 g          665 t
ORIGIN      
        1 ggggaaagcg cctgcggttc atgtcctcgg cggacgcggg agaaccggag cgcgaaggga
       61 aggcgaggcc gtagtcaacc tgccgccccg ccgactttcc gttttcttgg gatagctttt
      121 tactcaccgg tcctcttctc caccgccgaa gcattccgcg agtagcaaca catcgttacc
      181 gtgcgtgcct cactccgccc cgtaatgctc cgtcacgaga tcgatcattg ccgcgtcctc
      241 gtcgctgatc ccggtgaagg acgccccgat cccacgcccg ctggcgtagt agacggcctc
      301 ggctctcagc ttcagccacc gccccgtcgg caagagcacg cgcaggcaga tcgtctcggc
      361 ccgcagctcg tccaccaggg cctcgacgaa gcaccccccg cggctgaggc cctgcgcgat
      421 tccgacctcc tgacagtcgt cctcagccag cccccagaag gcgggcagcg gcacgtccat
      481 gcgctgcgcc ctcctgcgcc agaggtgctt cttctgccgt cccaagtccc cattcatagc
      541 ttccgcacgt aagcctccgg gtcttccctc ttcactttcg gcgagctatc gcccccggta
      601 cccgctcagc gtgaccagca gcagtgccgc gcccagcagc agcgtcgccg ccgcgctcag
      661 gccacgcagc cgccaccgtc gtgtgttcct gtagctgcgc ggatacgttc gcgacaggtg
      721 gctggaacgc gcgtccgcct tccccctgtc ggcgacgaag cgggcgagct gcccgaggcc
      781 gtgcagcagc agggcggcgg aggagagcgc catgacgagc agctcgtcct cgcgcccgag
      841 ccgtaggtcc ggtagtggcc agcccatagg tttccgacgc aagcatcgcg gtcttgcctc
      901 ttcaggtcga agcctccgcc ctacccactc aacggagccg cacgggcgtg acctcttcac
      961 ggccctgatg acgcgggcga ggaccagccc cagggcgacg cccgcccgcc gagtctacgg
     1021 cttcaaagct ccgtcccgaa tttatccagc aactcgggcc acttgacgtt gcccagggcg
     1081 ataaagtcgc cggaggacgc aagcaccctc tccttcaacg ccgcgcgagt tccttcgtcg
     1141 agcagcgccc acgctttccg taccgggcac cccgaggtgt gatccagccg cactttacag
     1201 acgggacacc cggcggcgct gatttcgttg gccgcgtaaa cggcggccag ccccgcgacg
     1261 atggcgttga tctgcttcag ctgcgggatg aggctggaat gagtcgccac gaccgcccac
     1321 tgccccccgt cgcacacgta cgtgttggtg aagcggtaca ggccgctgat gtcttcgtcc
     1381 ttgtaccgcg ccttgacggc gtcctgcccg aacgccaccg ccgcgccggg gtacaccttg
     1441 acggcctcga cccggcggat taaggactcg aacgtcagga ggccggactc gatcagtaag
     1501 agcaattccc ccttgccgaa tgagccggcg gcgcgcgtga gggtgaagtg ttcgggcagg
     1561 aggcgttcga gcgtggggat gtcgcgcctg acgtaagcct cggcccgctg gcgctccaga
     1621 cggatgatct cctgggcgac ggcgttgtcg gaggtgccgg tgcccccacc agccggttgt
     1681 gtactcactt ctttccgcct ttcttcacta cgcggcgtcc ttgccgccgc catcgcgccc
     1741 ggcccgctcg cgctgcaccc ggtagcggtg gttcatgtcg tcgagcaccg ccctcagccc
     1801 tgtgtcgggt gcgggctgcg ggggcgcctc tggactcttc accagtcgca gccggggctg
     1861 cgacttacgg taggcgggct ggtcggcctt cctcggagtg tcgtcgctca ctgattcccg
     1921 cccttctttc tgagaacggc gacgaggacg aaggcgattg ggatcagtgc gatgattatc
     1981 ccggtgctca tctctccccg cctttcttcg ccggcacggg gtcgaggagc gccgtcgtgt
     2041 tctccgtcac actcggcggg cgtaccaagt cttcggtgtc ggctgcgttc agtgcgggcg
     2101 gggcggcgcc ctgccgtgcc gggagtgcct ggctgcctcc gtccgactcc tgaccccgcc
     2161 tcccctgtgt gccgcgccct aatacgaacg cgacgccctg ccacacgacg accaccaggc
     2221 acaggaggat ggtggccctc agcaggtccg ggtgggcgcc gaagtgcacg gcgagcgtga
     2281 tcaaggcggc ggcggcggcg gcgagcgccg agaggagttg gaggagatgc accctccgct
     2341 gtctccgctc cggcgacagc ccccgcagcc tgccggcggg accgatcccc gcgcctgcgg
     2401 ggtcggggag ccactcgccg cagctccggc agtaggtccc gaccttctga ccggcggcgc
     2461 cgcacctggc gcagaagatg gccgcgtggc tcactcctcc ctgcctttct cccgcgggtc
     2521 atcatccggg ccgccgtctt cgtccagccg cccggccacc acgaccgccg ggacgcgcgc
     2581 gacgccgtaa ccggcggcca gcgccgcccc cctcgccgag tgcatatcga tcacacgcac
     2641 gtccacctcc tccccgtaga aggcgcgcac ccggcgcacc cgctcgcggc agagcggtca
     2701 gcccgcgctg aagacctcga ccctgagctt cggcttgatt tcactcactt ctccccacct
     2761 tccttctaag acgctggctt gtccgtgtcg tcttcggcca cttccatcag cacgccgagg
     2821 aagtggcgcg ccccgtccgc cgtcagacac gccggcccgg ctgcctccgg cgcccggctt
     2881 atggcggcct cgacctcggc gtccgtccac cggtcgaggt tgaagccgca ctcggcgaac
     2941 tgccgccgga agcgctcgag ttccgcgcgg cgccgccgcg accggagcca cgtccctagt
     3001 cccacttctt ccctcttcct tcctgctcgt cgaagccgcg ccggcccgcg ccgctacggg
     3061 atgcggctgg aatgcgtcac gacgatcacc caccgctcgc cgtcccacac gtaggtgttg
     3121 gtgaagcggt agcggccacc gatgtcctcg cccctgtacc gcaccttcac ctcgtccttc
     3181 ccgaacgcca ccgccgtcgc gaggtacacc ttgacggact cgacccgccg ctccagcgac
     3241 tcgaacgtca gctcgccgga ctcgatcagc gcgagcagct cgcctttccc gaacgagccg
     3301 atggaccggt tgaaggtgaa gtgctcgggc agcagccgcc cgagcgcggg gacgtcccgg
     3361 cgcaggtacg cctcggcccg cgccagttcg agccgggtga tctcgtcggt gactgcctgg
     3421 tcggaggcgc ccatgcccgc cgcggcagct tgtgtgctca tctcttcccg cctttccttt
     3481 tgcccgcagc cggagccacg gcacccagca tcaggccgct ggagtattcg ttaaaaaccg
     3541 tgtaagccgc cagcccgccc gccagcagcg aggccggcat ctcctgccgg gccgactccc
     3601 cgtggcaccg gtactcgacc acggccttac ctgcgcgccg cgggtccggc gtgaccgccg
     3661 actcctcgac gcgcctgctg caccgcgcgc accagggcga ctgcgacttg gggagcctgg
     3721 ccgccttcga ggccgcgcgc ccgatccgcc gcatgacccc ggcggtctgc ttagcccggc
     3781 aggccgcgca gtgccacccg acggccttgc cgccgagccg gtcggcggtc atcgcggagc
     3841 cgcactgctc gcaggtgggg gcgtcgctca cttcttcccg cctttcttct cgcccgtaga
     3901 aaccccgcta tcggcgcgtg ggactcatta acgcgcgccg gcgcagactc ctgcgccggc
     3961 gcgatcacga ccggctcagg agaccgtaaa a
//
LOCUS       DBNGOH010000106         2887 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4414514, whole genome shotgun sequence.
ACCESSION   DBNGOH010000106 DBNGOH010000000
VERSION     DBNGOH010000106.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2887)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2887)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2887
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4414514"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..394)
                     /locus_tag="AC159P_02030"
     CDS             complement(<1..394)
                     /locus_tag="AC159P_02030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694260.1"
                     /translation="MPLEEREWTIMFYFASDNPLAPEIVSQLKSIKQAGFHPEAHVVA
                     QFDPHTENTSTHIFDINRINKLKSGGEERIGFVGFNPNDPFVVNMMTDKIWGKEEDLD
                     GNLIRELIKQSLKAKGIRHNPPKLPARPP"
     gene            793..2490
                     /locus_tag="AC159P_02035"
     CDS             793..2490
                     /locus_tag="AC159P_02035"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HIR3694261.1"
                     /translation="MSSETFSRVAALSVVIAAALVGGMAGSRAFNRPSGATSAIANDY
                     SEALDLVAANYADEVNYDKAGEGAIQGMLTALDPHSNFFSQAEYRRLLQDQESRFTGI
                     GVSILRHRDGVYVQTPLAGTPAAKAGLRFGDRIVEVDGKDAREWTTQEVSKAVRGPEG
                     ERVTLKIERAGALAPLFFTVERAYVPQPSVRNTFMIRPGVGYVGLTGGFTKTTGGELK
                     KALDDLSREGARQIVLDLRNNPGGLLPESINVASHFIPRGETVVSVRGRDREEATRYE
                     NVSTDPVDHPLVVLINRNSASASEIVAGAIQDHGRGLIVGETSFGKGLVQKVFPLPLG
                     AGLTLTTAKYYTPYGRSIQRDYTGGSFYDYYVRHEPEPATPGQPTQVAPPNAPVNAPT
                     PQPTPAPKPNGPAIQTAGGRVYYGGGGITPDYEVKPLDLTRPTRARVFEASFHFVRQL
                     AGGQIAGLESYRISEGPQLGRYPRAGELAVNDKVLEAFREFVRRDKELGLSVAQVDAD
                     LDYVKLRVRDDLITAAYGGDAAGRFLLEGDPQILRALELLPVAKTLAENVGGAGARN"
     gene            2617..>2887
                     /locus_tag="AC159P_02040"
     CDS             2617..>2887
                     /locus_tag="AC159P_02040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694262.1"
                     /translation="MTTSHAVAIDEEADPERRYRDLARDERYGHSARIPARVCRCLEH
                     FGGLSDRAAVDERLRAYYVFVGVADDAIDAGSVHAGGRILEHLLAD"
BASE COUNT          457 a         1020 c          953 g          457 t
ORIGIN      
        1 gagggggacg ggcgggcagc ttcggcgggt tgtggcggat tcctttggcc ttaagagatt
       61 gcttgatcag ctctctgata aggttgccat ccaagtcctc ctccttaccc catatcttgt
      121 cggtcatcat gttgacgacg aaagggtcgt tggggttgaa cccgacgaac ccgatccgtt
      181 cttctccgcc ggatttgagt ttgttgatgc ggttgatgtc gaagatgtga gtcgaggtgt
      241 tctcggtgtg cgggtcgaac tgcgcgacga cgtgggcctc ggggtggaat ccggcctgct
      301 tgatggactt gagctgcgag acgatttcgg gggcgagcgg gttgtcgctc gcgaaataga
      361 acatgatcgt ccattctctc tcctctaggg gcatggctac tgtcctcctt cgctgtcgtg
      421 gaaacttcga gacctgcgac cccggaccgg caccacctcg tcaggctgct tcggccctga
      481 gcctgggaag ttcgggcttc tactctgaat gagttcgcgt gccgagatgt aacgggtgta
      541 aagggactgt ttcagggtcg tctgttcaag ctcggagggc ggggttcggg ccgcttccga
      601 cgttcgcatg gccgccgcct cgaatcctct ggcggccccg cccgcgggtt aggggcggca
      661 tattacacca cggcggcctc cgcgctaaag gaaaatcgtg aatgcggtgg gggccggggc
      721 ggtgtgctag aattctcttc gtcccccgcg cgcatgtcgg gcgggttcgc ctggggtctt
      781 tcggagtcga gcatgagcag tgaaactttt agccgcgtcg cggcactgtc ggtcgtcatc
      841 gctgccgcgc tcgtcggcgg catggccggg agccgcgcct tcaaccgccc gtcgggcgcg
      901 acctccgcga ttgccaacga ctacagcgag gcgctcgacc tcgtggccgc caactacgcc
      961 gacgaggtca actacgacaa ggccggcgag ggcgccatcc agggcatgct tacggcgctc
     1021 gacccgcact cgaacttctt ctcgcaggcc gagtaccggc ggctgctgca agaccaggag
     1081 tcgcgcttca cgggcatcgg cgtctcgatc ctgcgccacc gcgacggcgt ctacgtgcag
     1141 acgccgctcg caggcacgcc ggcggcgaag gcgggcctgc ggttcggcga ccgcatcgtc
     1201 gaggtggacg gcaaggacgc gcgcgagtgg acgacgcagg aggtgtcgaa ggccgtgcgc
     1261 ggccccgagg gcgagcgcgt cacgctcaag atcgagcgcg cgggcgcgct cgcgccgctc
     1321 ttcttcacgg tcgagcgcgc ctacgtccca cagccctctg tgcgcaacac gttcatgatc
     1381 cgccccggcg tcggctacgt cggcctcacg ggcgggttca cgaagacgac gggcggcgag
     1441 ctgaagaagg cgctcgacga cctgagcaga gagggcgcgc gccagatcgt cctcgacctc
     1501 aggaacaacc ccggcggcct gctgcccgaa tccatcaacg tcgccagcca cttcatcccg
     1561 cgcggcgaga cggtcgtctc cgtgcgcggg cgcgaccgcg aagaggcgac gcgctacgag
     1621 aacgtctcga ccgaccccgt ggatcacccg ctcgtcgtgc tcatcaaccg caactcggcc
     1681 tccgcctccg agatcgtcgc cggcgcgata caggatcacg ggcgcggcct catcgtcggc
     1741 gagacctcgt tcggcaaggg cctcgtgcag aaagtcttcc cgctcccctt aggcgcgggc
     1801 ctgacgctga cgaccgcgaa gtattacacg ccctacggtc gctcgatcca gcgcgactac
     1861 acgggcggct cgttctacga ctactacgtg cgccacgagc ccgaacccgc gacgcccggc
     1921 cagccgacgc aggtcgcgcc gccgaacgcg cctgtgaacg cgccgacccc gcagccgacg
     1981 cccgcgccga agccgaacgg ccccgcgatc cagaccgcgg gcgggcgcgt ctactacggc
     2041 ggcggcggca tcacgcccga ctacgaggtc aagccgctcg acctcacgcg ccccacgcgc
     2101 gcgcgcgtct tcgaggcgtc cttccacttc gtccgccagc tcgcgggcgg gcagatcgcg
     2161 ggactcgaaa gctaccgcat cagcgagggg ccgcagctcg gtcgctaccc gcgcgcgggc
     2221 gagctggccg tcaacgacaa agtcctcgaa gccttccgcg agttcgtccg ccgcgacaag
     2281 gagctgggcc tctcggtcgc gcaggttgac gcggacctcg actacgtcaa gctccgcgtg
     2341 cgcgacgacc tcatcaccgc cgcctacggc ggagacgccg ccgggcgctt cctcctcgaa
     2401 ggcgacccgc aaatcctccg cgcgctcgaa ctcctgcccg tggccaagac gctcgccgag
     2461 aacgtcggcg gcgcgggcgc gcgcaattaa gtccggcgcc gcgaaccacc ggctcgtcac
     2521 ccgcaccgcc cacggccctt ccggcgaacc gctcgaacag tcgcggcagt cgttcgcccc
     2581 ggcggggccg cgaaccacgc gccgagaata gaccacatga cgacttcgca cgccgtcgcc
     2641 atcgacgaag aggccgaccc ggagcggcgc taccgcgacc tcgcgcgcga cgagaggtac
     2701 ggccactccg cgcgcatccc cgcgcgcgtc tgccgctgcc tcgaacactt cggcggcctc
     2761 agcgaccgcg ccgcggtcga tgagcggctg cgcgcctact acgtcttcgt cggcgtcgcc
     2821 gacgacgcca tcgacgcggg cagcgtccac gcgggcgggc gcatcctcga acacttgctc
     2881 gcggacg
//
LOCUS       DBNGOH010000107         2040 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2215150, whole genome shotgun sequence.
ACCESSION   DBNGOH010000107 DBNGOH010000000
VERSION     DBNGOH010000107.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2040)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2040)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2040
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2215150"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1305
                     /locus_tag="AC159P_02045"
     CDS             <1..1305
                     /locus_tag="AC159P_02045"
                     /inference="COORDINATES: protein motif:HMM:NF051698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HIR3694263.1"
                     /translation="YDRIRGDLTIDAITNPPNVLQPTLFFGRLNDIPNLRGGGVRAIP
                     SVIGVDPGGELPTVYTYSLSVQRNVGWSTVVDVAYVGSQARHLARQRNLNAVPYLTAF
                     RREAQDPTRFAGGVVPAVEPNLPAAYSAAGFSFSGANALPVDFLRPFPGYGDILYRSF
                     DANSNYNGLQVGVTRRFKDNFTFGVAYTFSKTLTTSSDDNEITHPTDSRGYDYRLAGF
                     DRTHVLVVNYVYNLPKASRRLGDNWLARGLLDNWQLSGISQFVSGTPFELSLTGLGGL
                     GARLVGAPTVNAGNLSGQQPRFRLVGDPRMSGGTDGLQINPGAFAIPAIGDTGPYPRN
                     YLRNPGWNNHDLSVFKNFPFGGEGARYLQLRFEFFNILNHTQFTSFNGGAVTATPGST
                     TPVFNVRGSAACAATTTLGSCFGEFNAARDPRIVQLAAKLYF"
     gene            1447..>2040
                     /locus_tag="AC159P_02050"
     CDS             1447..>2040
                     /locus_tag="AC159P_02050"
                     /inference="COORDINATES: protein motif:HMM:NF028442.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HIR3694264.1"
                     /translation="MLSKVLLLPLLLAPVAHARQAGAGASVAELRRLFQAPPDDSRIM
                     MRWWWFGPSVTRENVEAEMRRMKEGGIGGFELAVVYPMALDDPARGLRNYPYLSPEYL
                     DMVRFAAAKARELGLRMDITIGSGWSYGGPYITPDLSAARLRSDRREITPDVTSLARP
                     VPFEGDRLMAAFVGRGSLQEIPSSFRELDVSGAGPIRL"
BASE COUNT          333 a          722 c          653 g          332 t
ORIGIN      
        1 tacgaccgca tccgcggcga cctcaccatc gacgcgatca cgaacccccc gaacgtcctg
       61 caaccgacgc tgttcttcgg gcgcttgaac gacatcccga acctgagggg cggcggcgtc
      121 cgtgcgattc cgagcgtcat cggcgttgac ccgggcggcg agctgcccac ggtctacacc
      181 tacagcctga gcgtgcagcg caacgtcgga tggagcaccg tcgtggacgt ggcctacgtc
      241 gggtcgcagg cgcgccacct cgcgcgccag aggaacctga acgccgtccc ctacctgact
      301 gccttcaggc gggaggcgca ggacccgacg cgcttcgcgg gcggcgttgt gccggcggtc
      361 gagccgaacc tgcccgcggc ctactcggcg gcgggattca gcttcagcgg ggcgaacgcg
      421 ctgccggtgg acttcctgcg gcccttcccc ggctacggcg acatcctcta cagatccttc
      481 gacgccaact ccaactacaa cgggctgcag gtcggggtca cgcgccgttt caaggacaac
      541 tttactttcg gggtggctta caccttctcg aaaacgctca ccacgtcgag cgacgataac
      601 gagatcacgc acccgaccga cagccgcggg tacgactacc gcctggccgg cttcgaccgc
      661 acgcacgtgc tcgtcgtcaa ctacgtctac aacctgccga aggccagccg ccgcctcggc
      721 gacaactggc tagccagggg gctattggac aactggcaac tgtcgggcat ctcgcagttc
      781 gtctcgggga cgccgttcga gctctcgctc accggactcg gtggcctcgg cgcgcgactc
      841 gtcggcgcgc ccacagtcaa cgccggcaac ctctccgggc agcagccgcg cttccgtctg
      901 gtcggcgacc cgaggatgag cggcgggacc gacgggctcc agatcaatcc gggcgccttc
      961 gccatcccgg ccatcggcga cacggggccg tacccgcgca actacctgcg caaccccggc
     1021 tggaacaacc acgacctctc ggtcttcaag aacttcccct tcggcggcga gggcgcgcgc
     1081 tacctgcagc tgcgtttcga gttcttcaac atcctcaacc acacgcagtt cacgagcttc
     1141 aacggggggg cagtcacggc gacgccgggt tcgaccacgc cggtcttcaa cgtgcgggga
     1201 agtgctgcct gcgccgcgac gaccacgctc gggtcatgct tcggcgagtt caacgccgcg
     1261 cgcgacccgc gcatcgttca gcttgcggcc aagctgtact tctaaccggg gcgcaaaccg
     1321 gcgcacgggt gaagacaggt cagtaccgcc cacggtagcg ggcggtactg acctacgcac
     1381 tcgatcgaag tggcggggag tgagtgaggg ggaaccgcga tgaaactttc gggccgttcg
     1441 agtctgatgt tgagtaaggt gctcctgctc cccctgctcc ttgcgcccgt ggcccacgcg
     1501 cggcaggcgg gtgccggcgc gagcgtcgct gaactccgcc gactcttcca ggcaccaccc
     1561 gacgacagcc ggatcatgat gcgctggtgg tggttcggcc cttcggttac gcgcgagaat
     1621 gtcgaggccg agatgcgccg gatgaaggag ggcggcatcg gcggcttcga gctggcggtc
     1681 gtctacccga tggcgctcga cgacccggcg cgcggccttc gcaactaccc gtacctctcg
     1741 ccggagtacc tcgacatggt caggttcgcc gccgccaagg cgcgcgagct ggggctgcgg
     1801 atggacatca ccatcggcag cggctggtct tacggcggcc cctacatcac gcccgatctg
     1861 tccgccgccc gcttacgttc cgaccgccgt gagattacgc ctgatgtcac cagcctcgcg
     1921 cggcccgtgc ctttcgaagg cgaccgcctg atggcggcgt tcgtcgggcg cggctcacta
     1981 caggaaattc cgagcagctt ccgcgagttg gacgtttcgg gagccgggcc catcaggctt
//
LOCUS       DBNGOH010000108         2939 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3865245, whole genome shotgun sequence.
ACCESSION   DBNGOH010000108 DBNGOH010000000
VERSION     DBNGOH010000108.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2939)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2939)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2939
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3865245"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1428)
                     /locus_tag="AC159P_02055"
     CDS             complement(<1..1428)
                     /locus_tag="AC159P_02055"
                     /inference="COORDINATES: protein motif:HMM:NF024753.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB
                     family protein"
                     /protein_id="HIR3694265.1"
                     /translation="MKKTDYEKLRRALVLVALLLAAVAAARPASARQQTGAQVPSQQL
                     KFGVFVARFNPDGTFTLQGDRWPSLGGNWRSEGGEIVLATAGGPEGCGGFGRYRMRVE
                     GERVSFDLVADDCRPRRMILDRSTWLPFGVTEKPVSRRIVRTAGGRAPARAVVGGAGG
                     SWPSFRGPQASGVAERQNLPDRWDGKTGENILWRTPIPGLAHSSPVVWGGRVYVTSAV
                     SSDPKATFRPGLYGDGDASSDRTRHRWVIYALDKRTGKVLWERTAHEGVPVDKRHIKS
                     TYANSTPATDGRIVVAWFGSQGVYAYDVNGRFLWKVDLGRLDLGAYDIPTYEWGPASS
                     PVIWNGLVIIQCDTQADSFVLALDANTGKTVWKTEREEIPSWGTPTVALTPNGPELVA
                     NASNFIRGYDPRTGKELWRLGRSSKITAPTPVFADGVFVVASGRAPERPIFVVRPGAR
                     GDLTLPEGKASSEWVAWSRTGRGSYM"
     gene            complement(1425..1958)
                     /locus_tag="AC159P_02060"
     CDS             complement(1425..1958)
                     /locus_tag="AC159P_02060"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016352244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3694266.1"
                     /translation="MPPEGLRIRRAEPDDYKALYEIFGGPKAFAGTLQLPYPSLEGWR
                     KRLAEPPEGLYNIVAVVGERVVGMLGVHTFPNKPRRRHVGAVGIAVHDDWQGNGVGSA
                     LMRAGLDLADNWLNLTRLTLEVYTDNEPAIRLYERFGFEREGTLRQDAFREGRYVDSY
                     TMARLRPAAAPDGVRRP"
     gene            complement(2025..>2939)
                     /locus_tag="AC159P_02065"
     CDS             complement(2025..>2939)
                     /locus_tag="AC159P_02065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HIR3694267.1"
                     /translation="WAPTAEDREHFFAEMYEHWGGPVGLEERAPSKANDPAFREWWAT
                     YLRMGASPGAAVALTKMNAEVDVRSVLPTVRVPTLVLHRAGDRCLKAAEGRYVAGLIP
                     RAKYVELPGEDHLPFVGDQDSMLEEIEEFLTGVRHTPEPERVLATILFARIADSPRRA
                     AEHGAERWQELLRRFRAHVSREVEWFRGREVDMEGEGPLATFDGPARAIRCACAISEY
                     ALRLGIEVRAGLHTGECDLLEGNRVGGVTVEIGREIRECAGPDEVLVSHTVRDLVAGS
                     GLDFEERDTETFANIPGRWRLYRVERGG"
BASE COUNT          420 a         1047 c          992 g          480 t
ORIGIN      
        1 catgtaagag ccgcggcccg tgcggctcca cgcgacccat tcgctgctcg ccttgccttc
       61 gggcagcgtc aggtcgccgc gcgcgccggg gcggacgacg aagatcgggc gctcgggcgc
      121 gcgcccgctg gcgacgacga acacgccgtc ggcgaagacg ggcgtcggcg cggtgatctt
      181 cgagctgcgc ccgagccgcc acagctcctt gcccgtgcgc gggtcgtaac cgcgtatgaa
      241 gttggacgcg ttggcgacca gctccgggcc gttcggcgtc aaggcgacgg tcggcgtgcc
      301 ccacgaaggt atctcttcgc gctcggtctt ccacacggtc ttgcccgtgt tcgcgtcgag
      361 cgccagcacg aacgagtcgg cttgcgtgtc gcactggatg atgacgaggc cgttccagat
      421 gacgggcgag ctggccggcc cccattcgta ggtcgggatg tcgtaggcgc cgaggtcgag
      481 ccgcccgaga tcgaccttcc agaggaagcg cccgttgaca tcgtaggcgt agacgccctg
      541 cgacccgaac caagccacga cgatgcgccc gtccgtcgcg ggtgtcgagt tggcgtaggt
      601 cgatttgatg tggcgcttat cgaccggcac gccttcgtgc gcggtgcgct cccagaggac
      661 tttgcccgtt cgcttgtcga gcgcgtagat cacccagcgg tgacgcgtgc ggtcgctcga
      721 ggcgtcgccg tcgccgtaga ggccggggcg gaatgtggct ttcgggtcgc tgctgacggc
      781 gctcgtgacg tagacgcgcc cgccccagac gaccgggctg gaatgtgcga gtccgggaat
      841 cggcgtgcgc cagaggatgt tctcgcccgt cttgccgtcc caacggtctg ggaggttctg
      901 ccgctcggcc accccggaag cctgcggacc gcggaaggaa ggccaactcc cgcccgcgcc
      961 gcccacgact gcgcgcgcgg gcgcgcgacc gcccgctgtc cgcacgattc gtcgagagac
     1021 aggcttctcc gtcaccccga agggcaacca ggtgctgcga tcgagaatca tccgtcgcgg
     1081 cctgcaatcg tccgcgacga ggtcgaagct gaccctctcg ccttcgacac gcatccggta
     1141 gcgcccgaat ccgccgcagc cctcggggcc gccggcggtc gccagcacga tctcgccgcc
     1201 ctcgctccgc cagttgccac cgagcgaagg ccagcggtcg ccctgtagcg tgaacgtgcc
     1261 gtcggggttg aagcgcgcga cgaagacgcc gaacttcaac tgttgggacg gaacctgcgc
     1321 gcccgtctgc tgacgtgccg acgcgggtct tgcggcggcg acggcggcca ggagcaaggc
     1381 tacgaggacg agcgcgcgcc ggagcttttc gtaatccgtt tttttcatgg tcttcgaact
     1441 ccgtccggcg cggcggcggg tcgcagccgc gccatcgtgt aggaatcgac gtagcgccct
     1501 tcacggaagg cgtcctgtcg cagcgtccct tcacgttcga agccgaagcg ctcgtagagg
     1561 cgaatcgccg gctcgttgtc cgtgtagact tcgagtgtca gtcgcgtcag gttgagccag
     1621 ttgtccgcca gatcaagccc cgcgcgcatc agcgccgacc cgacgccatt gccttgccag
     1681 tcgtcgtgca cggcgatgcc gacggcgccc acgtgccgcc gccgcggctt gttcgggaaa
     1741 gtgtggacgc cgagcatccc gaccacgcgc tcgccgacga ccgcgacgat gttgtacaga
     1801 ccctcgggcg gctccgcgag cctcttccgc cacccttcga gcgaagggta cggtaactgg
     1861 agcgtgcccg cgaacgcctt cggcccgccg aagatttcgt agagggcctt gtagtcgtcc
     1921 ggctcggccc ttctgattcg gagtccttcc ggtggcatcg cattcacccc tgaggacggg
     1981 cgggggcggc ccgaacgaac ttgagccgcc ccgccctgat tgattcaccc gccccgctcg
     2041 actctataca gacgccagcg tccggggatg ttagcgaagg tctccgtgtc gcgctcctcg
     2101 aagtcgaggc cggagcctgc gacgaggtct ctgaccgtgt gcgagacgag cacttcgtcc
     2161 ggccccgcgc actccctgat ctccctgccg atctccactg tgacgccgcc cacgcggttc
     2221 ccttcgagca ggtcgcactc gcctgtgtgc agcccggcgc ggacttcgat gccgagccgc
     2281 agcgcgtact cgctgatcgc gcaggcgcag cggatggcgc gcgcaggccc gtcgaaggtg
     2341 gcgagcggcc cctcgccctc catgtccact tcgcgcccac ggaaccactc gacctcgcgg
     2401 ctgacgtgcg cgcggaagcg ccggagtagt tcctgccagc gctcggcccc gtgctcggcg
     2461 gcgcggcgcg gcgagtcggc gatgcgcgcg aagaggatgg tggcgagcac gcgctcaggc
     2521 tccggcgtgt gccggacgcc ggtcaggaac tcttcgatct cttcgagcat cgagtcctga
     2581 tcgccgacga agggcaggtg gtcttcgccc ggaagctcga cgtacttcgc gcgcgggatc
     2641 aaccccgcga cgtagcgccc ctccgccgcc ttcaggcacc tgtcgcccgc gcggtgcagc
     2701 acgagcgtcg gcacgcgcac cgtcggcaag acgctccgca cgtccacctc cgcgttcatc
     2761 ttcgtcagcg ccacggcggc gccgggactc gcgcccatcc gcaggtacgt ggcccaccac
     2821 tcgcggaaag cggggtcgtt ggccttgctc ggcgcgcgct cttccagccc gacggggccg
     2881 ccccagtgtt cgtacatctc cgcgaagaag tgctcgcggt cttcggcggt cggcgccca
//
LOCUS       DBNGOH010000109         7159 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2031892, whole genome shotgun sequence.
ACCESSION   DBNGOH010000109 DBNGOH010000000
VERSION     DBNGOH010000109.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7159)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7159)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7159
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2031892"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            66..500
                     /locus_tag="AC159P_02070"
     CDS             66..500
                     /locus_tag="AC159P_02070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694268.1"
                     /translation="MTPDNTRQHSEWRERPERSYDHFRVRVRGRDAAGKIFKVMTYLD
                     NICEGGLYVRFDRAVAGGAPLSLFVSFSTLPHSEATSALRLSARGRVLRVESHPDGLC
                     GVAISFTRHRFVRNTTEATAVAPPTKAPAQADLSIEASRRNG"
     gene            515..3904
                     /locus_tag="AC159P_02075"
     CDS             515..3904
                     /locus_tag="AC159P_02075"
                     /inference="COORDINATES: protein motif:HMM:NF038114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HYR domain-containing protein"
                     /protein_id="HIR3694269.1"
                     /translation="MRKFLALALTVLVALAAAVCVTASPTAPLRQEAVRLQIDILPGV
                     APNTINLGDIQAVPVAILSTAGFDAASVNAASVTVGGAPVTKTDDGQLSVSRDVNEDG
                     LADVVIYFSPMFTQLDNSSTEAALRGSLNDGTPVEGSDAVQCSGRAPQTASAGPSVAP
                     LATIRPAPALALSLFGDIYNYIASFFAAPVETTQTFSNTANISIPASGNASPYPSNIS
                     VSGFPAAEKIKSVTVSLNKINHTAPDDLDILLVGPGGQKFILMSDRGGNNDLVNTNLT
                     FDDAAAALLPVNNIPGGTFRPTNQTNSGDNFSGVATPYLSPAPVGTATFFSAFGGASA
                     NGTWSLYVVDDLSGDSGNISGGWSITITTEPSDSTPPTISTPTVAPVEATGPSGAVVT
                     FAAPTANDNVDGAVTPTCDHFSGETFPVGTTRVNCSATDAAGNTGNAGFDVVVQDTTA
                     PAFSGTPASNTYEATSAAGAAATWTEPTATDAVDGARPVVCTPLAGSTFPLGTTTVNC
                     EASDNSSHTSSASFTIQVQDTTAPTLSVPANRTAEATSAAGAAVSFTASAADAVDATP
                     TVNCTPASGSTFALGATTVNCTATDAAGNVSNGSFTVTVKDTTGPAISGVPSDMTAEA
                     TGASGADVSYTNPTANDLVDGAVQVNCSPSSGATFALGATTVSCSAVDAAGNASSASF
                     KVTVQDTTAPALTVPPDQTEEATGASGATVNYPAATATDAVTSAPEITYSHASGSVFP
                     LGTTTVNVKAKDAAGNESSDTFTVTVEDTTAPIITVPLNRTAEATGATGAVVTFTAPS
                     ASDIVDGALTPNCVSAPAVNLSSGSTFPLGTTTITCNVSDAANNAAAQKSFTVTVQDT
                     TAPTLSGLTDKTIEATGPAGAVATYSPSATDLVDGGVTVNCSPVSGSTFAIGVATVNC
                     SATDAHGNTASGSFTVTVKDTTAPALNLPANITAQATSNSKAAVSYTATATDVVDGSV
                     SVNCAPASGSLFSVGTTTVNCSATDAHGNTANGSFTVAVSYNFNGFFQPVDNLPVLNK
                     AQAGSAIPVKFSLGGNQGLSIFATPNSPGSAMVGCGATSLVSEVEQTVTAGGSSLSYD
                     PVANQYVYVWKTDKSWAGTCRQLVVTFADGTVKGANFQFRK"
     gene            4143..4748
                     /locus_tag="AC159P_02080"
     CDS             4143..4748
                     /locus_tag="AC159P_02080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694270.1"
                     /translation="MRQTLRSLFIALALACASAPAFAQAGGDAGATAPSDEPAATHPI
                     LIHLAGGGRMEVDEANETADGVWYRRGNVTTLLDRARVVRVQREPDPVPAAAAPRADR
                     GEGSWNTPADLPRVEGFFRSAFKRPLPVSALGQSSVHNDWGYDHRHAVDIGLHPDGPE
                     GRALVQFLRGANIPFRTFRAAIPGVATGPHIHIGRPSRKFR"
     gene            5127..5531
                     /locus_tag="AC159P_02085"
     CDS             5127..5531
                     /locus_tag="AC159P_02085"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR3694271.1"
                     /translation="MTPPVNPTRERILFVDEHEDTCEAMTVLLSHRGYEVVTASTLIT
                     TLLTARGQPFNLYVLDAEFWGDGAELCRSIREFDPLTPIIFFSTAPDESARREAFEAG
                     ATDYVIKPDVDGLIESVSYCTSAKVCARESLY"
     gene            5848..6990
                     /gene="argE"
                     /locus_tag="AC159P_02090"
     CDS             5848..6990
                     /gene="argE"
                     /locus_tag="AC159P_02090"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008777 - acetylornithine deacetylase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HIR3694272.1"
                     /translation="MSVERTLAELVAIDSTSSRSNVEIVRFLAERAEGLGLCARLLPY
                     TDAAGVKKLQMVAVWPRSTRDDEVLELAFVGHTDTVPFDAAWAEALTLVEREGRLYGR
                     GACDTKGFIAAALAAIGATRLASLKRPLALVLTADEEVGCLGAKRLAEARAFRARHAV
                     VGEPTSLQPMRAGKGYCLADVTLRGREGHSAYPALGASAITRAARLVTGLERIAEELK
                     RDAHEAFDPPHTTLNVGRISGGTAKNVIAGECRFTLEWRPVPGQAPELVADLLRQEVE
                     RLREADEDFVCEIDVTRLDGGVETSADAPLVRALEEATGRVAGTIPFGTEAPQLAELG
                     ADAVVFGPGDIRVAHRTGEFVPASELRDCARILRRAIERFCGAEHQ"
BASE COUNT         1203 a         2595 c         2247 g         1114 t
ORIGIN      
        1 acaactccac gaataatccc aaggtaaaac cggtgcctcg aagggcgtac ggatagcgag
       61 acttgatgac gcccgacaac acccgtcagc acagcgaatg gagggagcga ccggagcggt
      121 cttacgacca cttccgggtg cgcgtcaggg gacgcgacgc cgcgggaaag attttcaagg
      181 tgatgacgta tctggacaac atctgcgagg gcgggttgta cgtgcgcttc gaccgcgcgg
      241 tcgcgggcgg cgcgccgctc tcgctcttcg tcagtttctc gaccctcccc cacagcgagg
      301 ccacgagcgc cctgcgcctc tccgcgcgcg ggcgcgtgct gcgggtcgag agccaccccg
      361 acggcctctg cggggtggcg atctcattca cgcgccacag gttcgttcga aacacaacgg
      421 aggcgaccgc cgtcgcccca cccacgaaag cgccggcaca ggctgacctc tcgatagaag
      481 cctcccggcg gaacggatga aaggattgcc ccaaatgaga aagtttctcg cgctcgcgtt
      541 aaccgtcctg gtcgctctgg ccgccgccgt ctgcgttacg gcgtctccga cggcccccct
      601 gcggcaggaa gccgtcaggc tacagatcga catcctgccc ggcgtcgccc cgaacaccat
      661 caacctgggt gacattcagg ccgtccccgt agcaatcctg agcaccgccg gttttgacgc
      721 cgcctcggtc aacgccgcca gcgtcaccgt gggcggcgcc cccgtgacga agaccgacga
      781 cgggcaactt tcggtgtcga gggacgtgaa cgaagacggc ctcgcggacg tggtcatcta
      841 cttctcgccg atgttcacgc agttggacaa cagctcgacg gaggccgccc tgcgcggcag
      901 cctgaacgac ggcacgcccg tcgaaggttc cgacgccgtc cagtgttcgg gacgcgcgcc
      961 gcagacggcc agcgccggcc ccagcgtcgc tccccttgcg acgatccggc ccgcccccgc
     1021 actggccctc agcctcttcg gcgacatcta caactacatc gcctccttct tcgccgcacc
     1081 cgtcgaaacg acgcaaacat tctcgaacac ggccaacatt tcgataccgg cctccggtaa
     1141 cgcaagcccc tacccgtcca acatttcggt ttcgggattt cctgccgcgg agaagatcaa
     1201 gagcgtcacg gtcagcctta ataagattaa ccacacggcc ccggatgatc tcgacatcct
     1261 gctcgtcggg ccgggggggc agaaattcat cctgatgtcg gaccgcggcg gcaataacga
     1321 tttggtgaat acgaatctta cgttcgacga cgcggcggcg gccctcctcc cggtcaacaa
     1381 tatccccggc ggcacgttca ggcccaccaa ccagaccaac tccggcgata acttttccgg
     1441 cgttgcgact ccttacctga gtcctgctcc ggtggggacg gcgactttct tttccgcctt
     1501 cggcggcgcc agtgcgaacg ggacgtggag cctttacgtg gttgacgacc tctcgggcga
     1561 ctccggcaac atctccggcg gttggtcgat cacgattacc accgagcctt cggactcgac
     1621 cccgccgacc atctcgacgc ccacggtcgc ccccgtcgag gccaccggcc cgtcgggcgc
     1681 ggtcgtgacc ttcgccgccc cgaccgccaa cgacaacgtt gacggcgccg tcacgccgac
     1741 ctgcgaccat ttctccgggg agaccttccc cgtcgggacg accagggtca actgctctgc
     1801 gaccgacgcc gcaggcaaca ccggcaacgc cggcttcgac gtggtcgttc aagacaccac
     1861 cgctcccgca ttctccggca cgcccgcgag caacacttat gaggcgacca gcgccgccgg
     1921 cgccgccgcc acgtggacgg agcccacggc gaccgacgcg gtggacggcg cgcgcccggt
     1981 cgtctgcacg cccctcgcgg gcagcacctt cccgctcggc acgacgaccg tcaattgcga
     2041 ggcgagcgat aacagcagcc acacctcttc ggcctcgttc acgatccagg ttcaggacac
     2101 gacggcgccc acgctgagcg tgccggccaa ccggaccgcc gaggcgacga gcgccgccgg
     2161 agccgccgtg agcttcacgg cttccgccgc ggacgccgtg gacgcgaccc ccacagtcaa
     2221 ctgcactccg gcttcgggca gcacgttcgc cctcggcgcg acgacggtca attgcacggc
     2281 gacggacgcg gcgggcaacg tctccaacgg cagcttcacc gtgaccgtca aggacacgac
     2341 cggccccgcc atctccggcg tgccgagtga tatgaccgcc gaggcaaccg gcgcgtccgg
     2401 cgccgacgtg agctacacga acccgaccgc caacgacctc gttgacgggg ccgttcaggt
     2461 caactgctcg ccgtcctcgg gcgcgacctt cgcgctcggc gcgacgacgg tctcctgctc
     2521 ggcggttgac gcagccggca atgcttcctc ggcctccttc aaggtgactg tacaggacac
     2581 gacggctccg gcgctgaccg tgccgcccga ccagaccgaa gaggctacgg gggcatccgg
     2641 cgcgacggtc aattaccctg cggcgacggc gaccgacgcc gtcacctccg ccccggagat
     2701 cacctactcg cacgcctcgg gcagcgtctt cccgctcggc acgacgaccg tgaacgtcaa
     2761 ggcgaaggac gccgccggca acgagtcttc cgacaccttc acggtgacgg tcgaggacac
     2821 gacggcccca atcatcacgg tgccgctgaa ccggacggct gaggcgacag gcgccaccgg
     2881 tgcggtagtc accttcacgg ccccttcggc ttccgacatc gtggacggcg cgctcacgcc
     2941 caattgcgtc agcgcgcccg cggtgaacct gtcctccggc tcgaccttcc cgctcggcac
     3001 gaccacgatc acatgtaacg tgagtgacgc ggccaacaac gccgccgccc agaagagctt
     3061 cacggtgacg gtccaagaca cgaccgcccc gacgctctcg ggtctcacgg acaagaccat
     3121 cgaggccacc ggcccggcag gcgcggtcgc cacctacagc ccgtcggcga ccgacctcgt
     3181 tgacggcggc gtgaccgtca actgctcgcc cgtctcgggc agcaccttcg ccatcggcgt
     3241 cgcgaccgtc aactgttcgg ccaccgacgc ccacggcaat accgccagcg gcagctttac
     3301 cgtgaccgtc aaggacacga ccgcgccggc gctcaacctt ccggcgaata tcaccgccca
     3361 ggccacgtcg aactcgaagg ccgcggtgag ctacaccgcg acggcgaccg acgtggtgga
     3421 cggtagcgtg agcgtgaact gcgcgccggc ctccggcagc ctgttctccg tcggcaccac
     3481 gacggtcaac tgctcggcga ccgacgctca tggcaacacc gcgaacggca gctttacagt
     3541 cgccgtgagc tacaacttca acggcttctt ccagcccgtt gacaacctgc ccgtcctcaa
     3601 caaggcgcag gccggtagcg cgatcccggt gaaattcagc ctgggcggta atcaggggct
     3661 gagtatcttc gcgacgccga actcccccgg ctctgcgatg gtcgggtgcg gcgcgacctc
     3721 tctggtgtcg gaggtcgagc agacggtcac ggctggcggc agcagcctca gctacgaccc
     3781 ggtcgccaac cagtacgtct acgtctggaa gactgacaag tcgtgggccg gcacctgccg
     3841 gcagctcgtc gtcacattcg ccgacggcac cgtgaagggc gccaacttcc agttcaggaa
     3901 gtaacccaca accccacgcg ccgggacgcc tttaccggat tggcgtagac gaaaacgggg
     3961 cggagactcc tcaggatatc tccgccccat ttcggtttct gctgttcggg aagccaccgc
     4021 cgcagcgggc aggcggaaaa cttcgccgcc cgaacacggg ggtattgatt tacctatcaa
     4081 tgccccctta tacttcttgc ggagcgtgcc tcccggcttg cgctccgccc cgaagatacg
     4141 tcatgcgaca gacactccgt tccctcttca tcgccctcgc gctcgcctgc gcgtcggccc
     4201 cggccttcgc ccaggccggc ggcgacgccg gcgcgaccgc gcccagcgac gagccggcag
     4261 ccacacaccc catcctcatt cacctcgccg ggggcggacg gatggaggtc gatgaggcga
     4321 acgagaccgc ggacggagtc tggtatcggc gcggcaacgt gacgaccctg ctcgaccgcg
     4381 cccgcgtcgt gcgcgtccag cgcgagcctg accccgtgcc cgccgccgcc gcccctcgcg
     4441 cggatcgggg cgagggcagt tggaacaccc cggctgacct cccccgggtc gaggggttct
     4501 tccgctccgc cttcaagcgc cccctgcccg tgagcgcgct cggccagtcg tccgtgcaca
     4561 acgactgggg ctacgaccac cggcacgcgg tggacatcgg cctccacccg gacggccccg
     4621 agggccgtgc gctcgtccag tttttgcgcg gcgcgaacat tcccttccgc accttccgcg
     4681 ccgcaatccc gggcgtggcg accgggccgc acatccacat cggtcggccc tcgcgcaagt
     4741 tccgctaagg tctctcccgc ttaccggctg agcaacccgc gccggttcct cgtccgacac
     4801 ctcccgcaaa ctcgtcttcg gccctccgcc ggttgacgct cgtgacaata atactagaca
     4861 gaattgggag ttttgtccgt tgtggccgcg cacccgtttg cttacactcc tcatcgctcg
     4921 acgcacgtgg ccgaccggcg atctgactcc cacacgctaa aacatgaagg ccgccgtcag
     4981 tgtgaatcaa gacggcccgc ttcgtgggcg gttatcgcgc cgggtcggta gtgctgtcgg
     5041 ccaccggcgc ccgcccgttc gggcacctcg accaaagccc ccgcgacgga tgcccctccg
     5101 ccttgagctt ccgcgcgcat actctgatga cgccccctgt gaacccgacc agagagcgca
     5161 tactcttcgt tgacgagcat gaggacacct gcgaagccat gacggtgctg ctgtcgcacc
     5221 gcggctacga ggtggtcacg gcatccaccc tcatcacgac gctgctcacc gcccgtgggc
     5281 agcccttcaa cctctacgtg ctcgacgcgg agttctgggg cgacggggcg gagctgtgcc
     5341 ggagcatacg cgagttcgac cccctcaccc cgatcatctt cttctcgacc gcgccggatg
     5401 aatcggcgcg gcgcgaagcg ttcgaggccg gggccaccga ctacgtcatc aagccggacg
     5461 tggacggcct gatcgagtcg gtgagctact gcacctccgc aaaggtgtgc gcgcgggagt
     5521 ccctctactg aacagccgcc gcgcgcctcg acgccgacca acccacggac acctcccacg
     5581 gattctccgg caggcgaatc gacccatcac ctgacatcgc gccgcggacg cacggcctcc
     5641 cacaggagcc gcggccccgt tcccgcacct cttttcgtgg aggaggccgg cgtgtgttgc
     5701 cgcgtatccc ttcatgctat ccttcccgct ccgtgaagct tttgcgcgtt cacacgaaaa
     5761 ttcacacaag ccgttgaaag cggctccggg cgggggccac gggaggcgag cgcttcccgt
     5821 tgccttccgc gccggagaat ggatgttatg agcgtggaac gaacactcgc cgagctggtc
     5881 gcgatagact ccacgtcttc ccgctccaac gtcgaaatcg tcagattttt agccgagcgc
     5941 gcggaaggct tggggctgtg cgcgcgcctt ctgccgtaca cggacgcggc gggcgtgaag
     6001 aagttgcaga tggtcgcggt ctggccgcgc tcgacgcgtg acgatgaagt gttggaactc
     6061 gccttcgtcg gccacacgga cacggtgcct ttcgacgcgg cgtgggcgga ggcgttgacg
     6121 ctggtcgagc gcgaggggag gttgtacggg cgcggggcgt gcgacacgaa ggggttcatc
     6181 gcggcggcgt tggcggccat cggggcgacg cgccttgcga gtttgaagag gccgctggcg
     6241 ctcgtcctga cggcggacga ggaggtgggg tgcctcggcg cgaagcgtct ggccgaggcg
     6301 cgcgcgttca gggcgcgtca cgcggtcgtc ggcgagccga cctctttgca gccgatgcgc
     6361 gcgggcaagg gctactgtct ggccgacgtg acgttgcgcg ggcgcgaggg ccacagcgcg
     6421 taccctgcgc tgggcgcttc ggccatcacg cgcgcggcgc ggctcgtcac cggccttgag
     6481 cgcatcgccg aggagttgaa gcgcgacgcg cacgaggcgt tcgacccgcc gcacacgacc
     6541 ctcaacgtcg ggcgcatcag cggcggcacg gcgaagaacg tcatcgcggg cgagtgccgc
     6601 ttcacgctcg aatggcggcc cgtgccgggg caggcgccgg agttggtggc agacctcttg
     6661 cggcaagagg tcgagaggct gcgcgaagct gacgaggatt tcgtctgcga gatcgacgtg
     6721 acgcggctcg acggcggggt ggagacgagc gcggacgcgc cgctcgtgcg cgcgctcgaa
     6781 gaggcgaccg gcagagtcgc gggcaccatc ccgttcggca cggaggcgcc gcagctcgcc
     6841 gaactcggcg cggacgcggt cgtcttcggc cccggcgaca tacgcgtcgc gcaccgcacg
     6901 ggcgagttcg tccccgcctc ggagcttcga gactgcgccc gaatcctccg ccgcgcaatc
     6961 gaacgctttt gcggcgcgga gcatcaataa ccagtcggcc cggattccag tcaatcgttt
     7021 taaggcgttc ccggatgttt ttccccagct tcaaaagact cccgacgagg ctcgcggcgc
     7081 tggcgctctg cgcgtttgtg ctcgcgggcg cttcggcgtc ggcgcggcag cagcagcagt
     7141 cgccgatgac caacgccga
//
LOCUS       DBNGOH010000110         1752 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4231278, whole genome shotgun sequence.
ACCESSION   DBNGOH010000110 DBNGOH010000000
VERSION     DBNGOH010000110.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1752)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1752)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1752
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4231278"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..475
                     /locus_tag="AC159P_02095"
     CDS             <1..475
                     /locus_tag="AC159P_02095"
                     /inference="COORDINATES: protein motif:HMM:NF042649.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HIR3694273.1"
                     /translation="MDERSAAWRRRLPKGHAVGFSCHESFGTGVACAAEVSLAGKRPR
                     IHRVTVAVDCGLAVNPLSVESQFQAGVAFGLSQLMAKGAVTLKDGVVEQRNFDGYVPP
                     YMPDAPVAVDVHIVPSAEPPSGCGEPPVPVISPAVVNALTRLTGKRYRSLPLVAV"
     gene            534..>1752
                     /locus_tag="AC159P_02100"
     CDS             534..>1752
                     /locus_tag="AC159P_02100"
                     /inference="COORDINATES: protein motif:HMM:NF036537.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LodA/GoxA family CTQ-dependent oxidase"
                     /protein_id="HIR3694274.1"
                     /translation="MDEVVRYVVYPCLGIARVGNSPDEYFIGPEAPGEVPQPEGGFKD
                     EAGRVKRQGARFRVYGLNKAGEAVREITADEAEITWRVHLANRKAGWYQFQNAMDLGP
                     KYAKTAAPRNRTITGGDREELIIDPGPRTIAGRDTQGDAYHFDSGEFMGKKVPLGELR
                     TDSQGRLVVLGGYGDSASYTGKPATTFANNEGWHDDTSDGPVRATVRVGGQEFEAEPA
                     AVAVTPPNYGQGLYGPVTMYDVVYDLFCRDAKFKTEAPKRPSFRRHIFPIIERLVGNQ
                     WVNGGVNFLFGAGSPSDLTAPSLLGQLSNPADEFEPLRTSYFNWFRDPADASGAQEPE
                     KITPFYGDAFGDYTGVGMDDLAVTPTQYRWLQQWAEGDFDTDASRELPPTLEDYPVAE
                     QPHALDEANLESCL"
BASE COUNT          305 a          620 c          574 g          253 t
ORIGIN      
        1 catggacgag aggagcgccg cgtggcgtcg caggctgccg aagggccacg ccgtcggctt
       61 ctcctgtcac gaatcgttcg gcaccggcgt cgcctgcgcc gccgaagtgt cgctcgcggg
      121 caagcgcccg cggattcatc gcgtgacggt cgcggtcgat tgcggcctcg cggtgaaccc
      181 gctctcggtc gagagccagt ttcaggcggg cgtcgcgttc ggcctgtcgc aactgatggc
      241 gaagggcgcg gtcacgctca aagacggcgt ggtggagcag cggaacttcg acggctacgt
      301 cccgccttac atgccggacg caccggtcgc ggtcgatgtc cacatcgtgc cgagcgcgga
      361 gccgccctcg ggttgcggcg agccgcccgt gcccgtcatc tcgcccgcgg tcgtcaacgc
      421 gctgacccgt ctgacaggga aacgttaccg cagcctgccg ctcgtcgccg tctagtctca
      481 cgcgacgggt tcacacgaac gagagcctca agagaaagga ccgaaaaacc ctcatggatg
      541 aagtcgtgcg ttacgtcgtc tatccctgcc tcggcatcgc acgcgtcggc aactcgccgg
      601 acgaatactt tatcgggccg gaggcgccgg gcgaggtgcc ccaacccgaa ggaggcttca
      661 aggacgaagc gggccgcgtc aagcgacagg gcgcgcgctt ccgcgtctac ggtctgaaca
      721 aggcgggcga agccgtcagg gagatcacgg cggacgaggc ggagatcacg tggcgcgtcc
      781 acctcgccaa ccgcaaggcc ggctggtatc agttccagaa cgcgatggac ttggggccga
      841 agtatgccaa gacggcggcg ccacgcaatc gcacgatcac gggcggcgac cgcgaggagc
      901 taatcatcga ccccggcccg cgcaccatcg ccgggcgcga cacgcagggc gacgcgtacc
      961 acttcgactc gggcgagttc atgggcaaga aggtgccgct cggcgagctg cgcaccgact
     1021 cgcaggggcg gctcgtcgtg ctcggcggct acggcgactc cgcctcgtac acgggcaagc
     1081 cagccaccac cttcgccaac aacgaaggct ggcacgacga cacctcggac ggccccgtcc
     1141 gcgccaccgt ccgtgtcggc ggacaggagt tcgaggccga gccggcggcg gtcgccgtca
     1201 cgccgcccaa ctacgggcag ggtctctacg gccccgtgac gatgtacgac gtggtctacg
     1261 acctcttctg ccgcgacgcg aagttcaaga ccgaagcgcc caagcgcccc tctttccggc
     1321 gtcacatctt ccccatcatc gaacggctcg tcggcaacca gtgggtcaac ggcggcgtca
     1381 acttcctctt cggcgcaggc tccccgtccg acctcaccgc gccctcgctg ctcggccagt
     1441 tgtcgaaccc ggcggacgag ttcgagccgc tacgcacttc ctacttcaac tggttccgcg
     1501 accctgcgga cgcttcaggg gcgcaggagc cggagaagat tacgcccttc tacggcgacg
     1561 ccttcggcga ctacacgggc gtcggcatgg acgatctggc cgtcacgccg acgcagtacc
     1621 gctggctaca gcagtgggcc gaaggcgact tcgacaccga cgcgagccgc gagctgccgc
     1681 cgacacttga ggactacccc gtcgccgagc agccgcacgc gctcgacgag gccaacctcg
     1741 aaagctgcct cg
//
LOCUS       DBNGOH010000111         3048 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1298939, whole genome shotgun sequence.
ACCESSION   DBNGOH010000111 DBNGOH010000000
VERSION     DBNGOH010000111.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3048)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3048)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3048
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1298939"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..651)
                     /locus_tag="AC159P_02105"
     CDS             complement(<1..651)
                     /locus_tag="AC159P_02105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694275.1"
                     /translation="MKRPRSLKQKLSDALPLSFKLSLKRVLRGLGFGGARDAKAAARR
                     DVIGREHGERDPAHAELAANSQHGPYVFVFDDRVPTPDRDAGSARMLFILKSLAEWSR
                     PVFVPLRSAWPEYEELLWEEGVETVGPLDYQRLLKRRKFHAAVLSRPEVAAGFLPSLR
                     RRDPSIKIVYDMVDAHFVRLAREHELTGDPETAREAARYRKTETRLARASDLVWCAS"
     gene            complement(789..2057)
                     /locus_tag="AC159P_02110"
     CDS             complement(789..2057)
                     /locus_tag="AC159P_02110"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3694276.1"
                     /translation="MKPVVRIENVGKRYLIGGLHPGYLTFREMLGGLVAAPFKRRRGA
                     GARRQALWALKEIDLEVGEGELVGVIGHNGAGKSTLLKILSRITKPTTGEVELRGRVG
                     SLLEVGTGFHPDLTGRENIYLNGAILGMKRAEIRRKFDEIVSFAEIEKFLETPVKWYS
                     SGMYVRLAFSVAAHLEPEILMMDEVLAVGDAAFQQKCLDKMRDIRRQGRTIFFVSHDM
                     AAITRLAKRVVLLEGGRVVSDGPAQQVVRDYLSSNWMAGAERAWDDAAAAPGNHVARL
                     RRVRVRTEDGETTAAVDIRRAVGVEVTYDVLEEGHALSPVVELYNEEGTELFSTHDTG
                     EEWRRRPRARGRYTSTVWMPGNLLAEGSLIANVSLMSHHPSTVLHAHEQNAVAFQVID
                     SQSGDSARGDYVGPMPGVVRPLLRWETKRF"
     gene            complement(2179..>3048)
                     /locus_tag="AC159P_02115"
     CDS             complement(2179..>3048)
                     /locus_tag="AC159P_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3694277.1"
                     /translation="PPAASDAGAERAPATPARAPRVLIKSEESGVQLNLGDWWAYREL
                     LYFLTLRDVKVRYKQTALGAAWVIVQPLFMMLIVTLVFNRFARVGGGELPYPLFAYAG
                     LLVWVFFSNAVVNGTNSLTLNTNLITKVYFPRIFIPAAAVGAGLVDLSIASLVLAALA
                     VYYGVGFHAGLLLLPFFVLLATLLALGAGVLVSALTVKYRDLRHALPFVIQFLMFASP
                     VIYPPSIVPESWRWLLVANPLAGILEGFRSALTGRPFDWPAIAVAALATTALLVVSVY
                     VFRRTEDTFADVI"
BASE COUNT          501 a          975 c         1083 g          489 t
ORIGIN      
        1 cgaggcgcac cagacgaggt cgctcgcgcg cgcgagccgc gtctcggtct tgcggtagcg
       61 cgccgcctcg cgcgccgtct cggggtcgcc cgtcagttcg tgctcgcgcg cgaggcggac
      121 gaagtgcgcg tccaccatgt cgtagacgat cttgatcgag gggtctcggc ggcgcagcga
      181 cggcaggaac ccggcggcga cctcggggcg gctgaggacg gcggcgtgga actttcgccg
      241 cttcaggagc cgctggtagt cgagcgggcc gacggtttcg acgccctcct cccacaaaag
      301 ttcttcgtac tcgggccacg cgctccgcag ggggacgaag acgggcctcg accactcggc
      361 gagcgacttc aggatgaaga gcatgcgcgc gctgcccgcg tcgcggtcgg gcgtcggcac
      421 gcggtcgtcg aagacgaaga cgtaggggcc gtgctgactg ttcgcggcga gttcggcgtg
      481 cgccgggtct ctctcgccgt gctcgcgccc gatcacatcg cgccgtgcgg cggccttcgc
      541 gtcgcgtgcg ccgccgaaac cgaggccgcg cagcacgcgc ttgagagaga gcttgaacga
      601 gaggggcagc gcatcactga gcttctgttt gagtgaacgg ggtcgcttca tgtgttcgga
      661 agcttcgagc ggcctgcgga ttttggtttt agatttcgga tttcggattg gccgttgatg
      721 gttgacggga gcggcgctgt catcaatcgt cggtcatcgt cgtcgaacac taatccgaaa
      781 tccgaaatct aaaatcgctt cgtctcccat ctgagcagcg ggcggacgac gccgggcatc
      841 gggccgacgt agtcgccgcg cgccgagtcg cccgactggc tgtcgatgac ctggaaggcg
      901 acggcgttct gctcgtgcgc gtggagcacg gtcgaggggt ggtgcgacat cagggagacg
      961 ttggcgatga ggctgccttc ggcgagcagg ttgccgggca tccagaccgt gctcgtgtag
     1021 cgaccgcgcg cgcgcgggcg gcggcgccac tcttcgccgg tgtcgtgcgt gctgaacagc
     1081 tcggtgccct cctcgttgta cagctcgacg acgggcgaga gcgcgtggcc ctcttcgagc
     1141 acgtcgtacg tgacctcgac gccgaccgcg cggcggatgt ccacggcggc ggtcgtctcg
     1201 ccgtcctccg tgcgcacgcg gacgcggcgc aggcgcgcga cgtggttgcc gggggccgcc
     1261 gccgcatcgt cccacgcgcg ctcggccccg gccatccagt tcgagctgag gtagtctcgg
     1321 acgacctgct gcgccggccc gtcgctcacc acgcgcccgc cttcgaggag cacgacgcgc
     1381 ttcgccagcc gcgtgatggc ggccatgtcg tgcgagacga aaaagatggt gcgcccctgc
     1441 cggcggatgt cgcgcatctt gtcgaggcac ttctgctgga aggccgcgtc gccgaccgcg
     1501 agcacctcgt ccatcatcag gatttccggt tcgaggtgcg ccgccacgga gaaggcgagc
     1561 cgcacgtaca tgcccgacga gtaccacttg acgggcgtct ccaggaactt ctcgatctcg
     1621 gcgaaggaga cgatctcgtc gaacttgcgc ctgatctcgg cgcgcttcat gccgaggatc
     1681 gcgccgttca gatagatgtt ctcgcgcccc gtgaggtccg ggtgaaagcc cgtgccgact
     1741 tcgagcaggc tgccgacgcg gcccctcagc tccacctcgc cggtcgtcgg cttggtgatg
     1801 cgcgagagga ttttcagcag cgtacttttc cccgcgccgt tgtgcccgat gacgccgacc
     1861 agctcgccct cgccgacttc gaggtcgatc tctttcagcg cccacaacgc ctgccgccgc
     1921 gccccggcgc cgcgccggcg tttgaacggc gccgcgacga ggccgcccag catctcgcgg
     1981 aacgtcaagt agcccggatg cagcccaccg atcaggtagc gcttgccgac gttctcgatt
     2041 ctgacgactg gcttcatacg gtaagaaggg ggaaaagggg aaaaggggaa aaggggaaga
     2101 gcttaagagg ttgagtgtcg ttgaagcgca tcgtgaatcc gttttccttt tcgcctttta
     2161 accttttccc cttgtctttt aaatcacgtc ggcgaaggtg tcctccgtgc ggcggaaaac
     2221 gtagacggag acgacgagga gcgcggtcgt cgccaacgcg gcgaccgcta tcgcgggcca
     2281 gtcgaagggg cggcccgtca gcgcggagcg gaagccttcg aggatgcccg cgagcgggtt
     2341 ggcgacgagc agccagcgcc agctctccgg cacgatggac ggcgggtaga tgaccggcga
     2401 ggcgaacatc aaaaactgga tgacgaaggg gagcgcgtgc ctgaggtcgc ggtacttgac
     2461 cgtgagcgcc gagacgagca cccccgcgcc gagcgcgagc agcgtcgcca gcaggacgaa
     2521 gaaggggagc agcaacagcc ccgcgtgaaa gccgacgccg taatagacgg cgagcgccgc
     2581 gagcacgagc gacgcgatgg agaggtcgac cagccccgcc ccgaccgccg ccgcagggat
     2641 gaagatgcgc gggaaataga ccttggtgat gaggttcgtg ttgagggtga ggctgttggt
     2701 gccgttgacg accgcgttcg agaagaagac ccagacgagc agccccgcgt aggcgaagag
     2761 cgggtagggc agctcgcccc cgcccacgcg cgcgaagcgg ttgaagacga gcgtcacgat
     2821 gagcatcatg aagagcggct ggacgatgac ccacgccgcc ccgagcgccg tctgcttgta
     2881 gcgcaccttc acgtcgcgca gggtgaggaa gtagagcagc tcgcggtagg cccaccagtc
     2941 gccgaggttg agctgcacgc ccgactcctc ggacttgatg agaacgcgcg gggcgcgggc
     3001 cggcgtcgcc ggggcgcgct cggctccggc gtcgctcgcg gcgggcgg
//
LOCUS       DBNGOH010000112         7792 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3131701, whole genome shotgun sequence.
ACCESSION   DBNGOH010000112 DBNGOH010000000
VERSION     DBNGOH010000112.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7792)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7792)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7792
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3131701"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(44..1129)
                     /locus_tag="AC159P_02120"
     CDS             complement(44..1129)
                     /locus_tag="AC159P_02120"
                     /inference="COORDINATES: protein motif:HMM:NF012801.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor domain-containing
                     protein"
                     /protein_id="HIR3694278.1"
                     /translation="MQQHDSRLQQGANAQYLRPYKLAGLQALLTAGGNYHDNQINVGL
                     SRTEARAPFEAVTGAHGRVTNGGLYVQHALELMHGHLRVEGGLRLDYFRFDVDDRLSP
                     ALSGRHGAALLQPKLSVAFTPADKLPATIYLNYGRGISSQDARGVVRDPSAPKVSTTD
                     FYQLGTAHNLSRFSLSADLFLIERSHEQTYVPDDGSFEFKGRSRSYGFEARASARLTR
                     HLAFNGGFTKVGNAFFRGTTPRVYVDAAPHRVANAALTLSRLEGFSASLGYRHVGGYR
                     LDGEDARPRAAGLDVLDFSLTKRITRALDFNLSADNLTNKRYYETQNYFESRTRPGDP
                     AAPRVHGTPGYPFGVTVGVTLRLFGKD"
     gene            1052..1237
                     /locus_tag="AC159P_02125"
     CDS             1052..1237
                     /locus_tag="AC159P_02125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694279.1"
                     /translation="MQTGEFVGPQILRVRALLQTRVVLLNLVAARLVVEEESEIRVEV
                     EERAGGEAVELERAAGL"
     gene            complement(1232..1690)
                     /locus_tag="AC159P_02130"
     CDS             complement(1232..1690)
                     /locus_tag="AC159P_02130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694280.1"
                     /translation="MDILNGPFSAQYGDFSGLGVVRVRLKESLPDRLTLRLQGGSFDT
                     CRTFVAYSPRLKEADSFVAYEASRTDGPFLNPLRYGRDNVTANYTRRRGEGHAWGVKF
                     NFGRNDSVSSGQLPLDEVAAGRLDRFGFIDPSGGGRVRAGVLGVYYRREL"
     gene            complement(1724..2347)
                     /locus_tag="AC159P_02135"
     CDS             complement(1724..2347)
                     /locus_tag="AC159P_02135"
                     /inference="COORDINATES: protein motif:HMM:NF025006.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HIR3694281.1"
                     /translation="MCGNLKRAVAGFCLALCLCPFAAAQEAPSRTVAGVVVTPQNEVV
                     PGVSVLARHASGERRAETDGEGAFRLAVPAGPVTLTFEGKGIAPLERTIGAGENAEGL
                     RIRVTFVVPPVHESVVIEATALDPSIDRRNAAVYEDTLFARDDQLLQTLAAGINAGQH
                     EGGGKSLEVRRFGFNLDHGGVGGGLKVLVDNVQQNQGTQGHGQGYLG"
     gene            complement(2394..3905)
                     /locus_tag="AC159P_02140"
     CDS             complement(2394..3905)
                     /locus_tag="AC159P_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014398456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HIR3694282.1"
                     /translation="MADTNDEKRRAAGDAYERELATDLKDQTPDRPRGMPLHTRILIG
                     LLAGVVAGVATYMLFRVPDPKIAGNFVPHPRVEWVIGNITEPVGTLFLRLLLMVVVPL
                     VFSSLVVGVAGIGDIRRLGRVGLKSFAYTFIISAISVVIGITLANTLRPGERLDAGTR
                     AGLEARYSADATRRVADATKAGTTDSALMQVVKTIVPENPVASAAGIIRGEEKQTDTP
                     NMLHLMFFAVLLGVAATLVPVEVSAPLLRVLQALFEIAAKIIDLIMKLAPFAVACLLF
                     NNTARFGLDLLGALAWFVATVLLGLSLHMFGVYSLSVYFLSRISPLEFFRRIKTVMLT
                     AFSTSSSNATLPTALRVSEENLGVPREINSFVLTVGATANQNGTALYEGVTVLFLAQL
                     AGVELSLASQLGIAYFAILGGIGTAGVPSGSIPFIIIMLASYNIPPALIAIILGVDRI
                     LDMCRTTLNVTGDITAATYVARSEGYELLRPHVPELERRGVVVGGPSSHDVLD"
     gene            complement(3997..4881)
                     /locus_tag="AC159P_02145"
     CDS             complement(3997..4881)
                     /locus_tag="AC159P_02145"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF039482.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006741 - NADP+ biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019674 - NAD+ metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HIR3694283.1"
                     /translation="MEKPSIRRVGVLVKPHQPEALKTICRLAEWCARHGIALVGGPKI
                     DRTAVEAESGCTIEKMKHEELVDSVDLIVVLGGDGTMIGAARMLGDRGVPVLGVNYGT
                     FGYLAEFRQEELEAALDAVLAGVYKIDRRLMLAAEVYRRGKLIEHNRVLNDVVISKSA
                     LARIIEIETRIDGLLVNCFRADGLIISTPTGSTAYNLSAGGPVIYPSMNAVVITPICP
                     HTLSNRPLVVPDDMEFELILKTPREEVALTLDGQVGVALEVEDRVRVRKSRASFNLVQ
                     ADTRNYFEVLRNKLKWGR"
     gene            complement(5144..5617)
                     /locus_tag="AC159P_02150"
     CDS             complement(5144..5617)
                     /locus_tag="AC159P_02150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694284.1"
                     /translation="MFCQHCGTEANVDLNYCKRCGGNLGGVAALTTAPEMRPAITTGT
                     ACAVGLSMLLLVVMGLGLLLAFISEMRGSGLPPDVFKMIVTFGAFTILGGVASLTWLW
                     TYLLGGARRLPTTPQLKAPPAAGELGPARVGGALPERPPASVVEHTTRTLEHSRR"
     gene            complement(5765..6433)
                     /locus_tag="AC159P_02155"
     CDS             complement(5765..6433)
                     /locus_tag="AC159P_02155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694285.1"
                     /translation="MRKLTEEAALARLQRSKAAYQSRVKLNFERAARDCGACPTKGVC
                     CTDAHFVNVHITRLEAVAIRETLARTPRLTEEERRAVYARARAAVERYGLARAGGDTF
                     ARTYSCPLFEPGAGCLVHRRAKPAPCIQHACYDRWEDVPPLDLQWREERRVEQLNNEV
                     YGAAWAWLPTPLWLTLVDPSSDGAELARLARAWGVRGAHDHQRGNAARARRLKNRTLP
                     VLRA"
     gene            complement(6443..6940)
                     /locus_tag="AC159P_02160"
     CDS             complement(6443..6940)
                     /locus_tag="AC159P_02160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694286.1"
                     /translation="MAFILTKDRPRNSDEEWNAAWEAYSNYIQRIKDRLPRSAYEFAT
                     APWHYDFTDHKSPHDGWLEAITIREPSHGERQQYRTVEIAVRLLAAYHDGQIELVYTN
                     VYSYSLDLPAVALPADNRGHRDWLYDEIGLSEQGNVLHEIEWSRAGGWLIECEDISYR
                     WKPFG"
     gene            complement(6967..7719)
                     /locus_tag="AC159P_02165"
     CDS             complement(6967..7719)
                     /locus_tag="AC159P_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015836884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HIR3694287.1"
                     /translation="MKRERVDKLLVERGLAPSRTRAQALVMAGVVLAGERRVEKPSET
                     VAPDAPLRVRGSEDPAARYVGRGGLKMEKALREFGLDVRGLVCLDVGASTGGFTDCLL
                     QHGARRVVALDVGHNQIDWRLRTDPRVEVREGVNARHLRPADFGTERFDLAVMDVSFI
                     SAAKVMPAVVPLLEQGAHLVVLIKPQFEVGRGEVGKGGVVRDPARHARVVEEVNAAAR
                     ALGLGVRGVTDSPITGADGNREFLALYEKSRG"
BASE COUNT         1271 a         2506 c         2756 g         1259 t
ORIGIN      
        1 ttctgctgct cggcggtagg tgcgtcgctt cgcctcgcgc ccttcagtcc ttgccgaaga
       61 ggcggagggt gacgcccacc gtgacgccga aggggtagcc gggcgtgccg tggacgcgcg
      121 gggcggcggg gtcgccgggg cgcgtgcgcg attcgaagta gttctgcgtc tcgtagtagc
      181 gcttgttcgt caggttgtcg gcggagaggt tgaagtcgag ggcgcgcgtg atgcgtttcg
      241 tcaggctgaa gtccagcacg tcaagccccg cggcgcgcgg gcgcgcgtct tcgccgtcga
      301 gccggtagcc gccgacgtgg cggtagccga gcgaggcact gaagccttcg agccgcgaca
      361 gcgtgagcgc cgcgttggcg acgcggtgcg gggccgcgtc cacgtagacg cgcggcgtgg
      421 tgccgcggaa gaaggcgttg ccgactttgg tgaagccgcc gttgaaggcg aggtggcgcg
      481 tcagccgcgc ggaggctctg gcctcgaacc cgtaggagcg gctgcggccc ttgaactcga
      541 agctgccgtc gtctgggacg taagtctgtt cgtgcgagcg ttcgatgagg aagaggtcgg
      601 cgctcagcga gaagcggctc aggttgtgcg ccgtgccgag ctggtagaag tcggtcgtcg
      661 agactttggg ggccgagggg tcgcgcacga cgccgcgcgc gtcctgactt gagatgccgc
      721 gcccgtagtt gagatagatg gtggccggga gtttgtccgc aggcgtgaag gcgacgctca
      781 gtttcggttg caggagggcg gcgccgtgcc gcccggagag cgccgggctg agcctgtcgt
      841 caacgtcgaa gcggaagtag tcgaggcgca ggccgccttc gacgcggagg tggccgtgca
      901 tgagttccag tgcgtgctgg acgtagaggc cgccgttcgt gacgcggccg tgcgcgcccg
      961 tcaccgcttc gaagggcgcg cgcgcctctg tgcggctgag gccgacgttg atctggttgt
     1021 cgtggtagtt gccgccggcg gtgaggagcg cttgcagacc ggcgagtttg tagggccgca
     1081 gatactgcgc gttcgcgccc tgttgcagac gcgagtcgtg ctgctgaatc tcgtcgccgc
     1141 gcgtctcgtc gttgaggaag aaagtgaaat tcgagtagag gtcgaagagc gcgcgggcgg
     1201 cgaagccgtc gagcttgaac gagccgccgg actatagctc cctgcggtaa tacacgccga
     1261 ggactccggc gcgcacacgc ccgccgcccg acgggtcgat gaagccgaag cggtcgagtc
     1321 ggcccgcggc cacttcgtcg agcgggagct ggccggagga gacggagtcg ttgcgcccga
     1381 agttgaattt gacgccccac gcatggcctt cgccgcgccg ccgcgtgtag ttggccgtca
     1441 cgttgtcacg cccgtagcgc agcgggttga ggaaggggcc gtcggtgcgt gaagcctcgt
     1501 aggcgacgaa cgagtccgct tccttcagtc gggggctgta ggcgacgaag gtgcggcagg
     1561 tgtcgaacga gccgccctgc aagcgtaggg tgaggcggtc gggcagcgac tctttgaggc
     1621 ggacgcggac gacaccgagg ccggagaagt cgccgtactg cgcgctgaac ggcccgttca
     1681 ggatgtccac gtccttgacg agttcgggtg tcaggctctt gagctacccg aggtagccct
     1741 gcccgtgccc ctgcgtgcct tgattctgct gcacgttatc gacgagcacc ttcagcccgc
     1801 cgcccacgcc gccgtggtcg aggttgaagc cgaagcggcg gacttcgagc gacttgccgc
     1861 cgccctcgtg ctggccggcg ttgatgcccg cggcgagcgt ctgcaagagc tggtcgtcgc
     1921 gcgcgaagag cgtgtcttcg tagacggcgg cgttgcgccg gtcgatggag gggtcgagcg
     1981 ccgtggcctc gatgaccacg ctctcgtgca cgggcgggac gacgaacgtg accctgattc
     2041 gcagaccctc ggcgttctcg cccgcgccga ttgtcctctc aagcggcgcg atgcctttgc
     2101 cctcgaaggt cagcgtgaca ggcccggcgg gcaccgcgag gcggaacgcg ccctcgccgt
     2161 cggtctcggc gcgccgctcg cccgaagcgt gacgcgcgag cacggagacg cccggcacga
     2221 cctcgttctg cggcgtcacg acgacgcccg cgacggtgcg cgacggggcc tcctgcgcgg
     2281 cggcgaaagg gcagaggcag agcgcgaggc agaatccggc gaccgcgcgt ttcaggtttc
     2341 cacacatctc acgttcgcgc ttgccacgcg tcggcgttct acgttttcag ccctcagtcc
     2401 aaaacgtcgt ggctcgacgg gccgcccacg acgacgccgc gtcgctcaag ctcgggcacg
     2461 tgcggcctca gaagctcgta gccttccgag cgcgcgacgt aggtcgccgc cgtgatgtcg
     2521 cccgtgacgt tcagcgtcgt gcggcacatg tcgagaatcc tgtccacgcc gaggatgatg
     2581 gcgatgagcg cgggcgggat gttgtacgag gcgagcatga tgatgatgaa cgggatggaa
     2641 cccgacggca cgcccgccgt gccgatgccg ccgaggatgg cgaagtaggc gatgccgagc
     2701 tgcgacgcga gcgacaactc gacgcccgcg agctgcgcga ggaagagcac cgtgacgccc
     2761 tcgtagagcg ccgtcccgtt ctggttggcg gtcgcgccca cggtcagcac gaagctgttg
     2821 atctcgcgcg ggacgcccaa attctcctcc gacacgcgga gcgccgtggg cagcgtcgcg
     2881 ttcgaagagg aggtggagaa ggccgtcagc atgacggtct tgatgcggcg gaagaattcg
     2941 agcggagaga tgcgcgagag gaagtagacc gacagcgagt agacgccgaa catgtggagc
     3001 gagaggccca ggagcaccgt cgccacgaac cacgcgagcg cgcccagaag gtcgaggccg
     3061 aaccgcgccg tgttgttgaa gagcaggcac gcgacggcga acggcgccag cttcatgatg
     3121 aggtcgatga tcttggccgc gatctcgaag agcgcctgga ggacgcgcag cagcggcgcg
     3181 ctgacctcca cggggacgag cgtcgcggcc acgccgagca gcaccgcgaa gaacatcagg
     3241 tggagcatgt tcggcgtgtc ggtctgcttc tcctcgcccc tgatgatgcc cgcggcggac
     3301 gccacagggt tctccggcac gatggtcttg accacctgca tcagcgccga gtcggtcgtc
     3361 ccggccttcg tcgcgtcggc cacgcgtcgc gtcgcgtcgg cgctgtagcg cgcctcgagg
     3421 ccggcgcgcg tcccggcgtc gagccgctcg ccgggccgga gcgtgttcgc cagcgtgatg
     3481 ccgatgacga ccgagatggc cgagatgatg aacgtgtagg cgaacgactt cagcccgacg
     3541 cgcccgagcc gccggatgtc gccgatgccg gcgacgccca ccaccagcga cgagaagacg
     3601 agcggcacga cgaccatcag cagcagccgc aggaagagcg tccccaccgg ctccgtgatg
     3661 ttgccgatga cccactccac ccgcgggtgc gggacgaagt tccccgcgat cttggggtcg
     3721 gggacgcgga acagcatgta cgtcgccacg cccgccacca cacccgccag cagcccgatc
     3781 aggatgcgtg tgtgcagcgg catcccccgc ggcctgtccg gcgtctggtc cttcaggtcg
     3841 gtcgccagct cgcgctcgta agcgtcgccc gccgcccgcc gcttctcgtc gttggtgtca
     3901 gccatagcaa ctaccgtgaa ccgtgaaccg taatccgtga taagagaaaa cggattctga
     3961 cttatcacgg tttacggttc acggcttacg gccttttcac ctcccccact tcaatttgtt
     4021 ccgcagaact tcgaagtagt tgcgcgtgtc ggcctggacg aggttgaagc tggcgcggct
     4081 cttgcgcacg cggacgcggt cttcgacttc gagcgcgacg ccgacctgcc cgtccagcgt
     4141 gagcgcgacc tcctcgcgcg gcgtcttcag gatgagttcg aactccatgt cgtcgggcac
     4201 gacgagcggg cggttcgaga gcgtgtgcgg acagatgggc gtgatgacga cggcgttcat
     4261 cgaggggtag atgacggggc cgcccgccga gaggttgtag gccgtcgagc cggtcggcgt
     4321 cgagatgatg aggccgtcgg cgcggaagca gttgaccagc agcccgtcga tccgcgtctc
     4381 gatctcgatg atgcgcgcca gcgccgactt cgagatgacc acgtcgttca ggacgcggtt
     4441 gtgctcgatc agtttgccgc ggcggtagac ctccgccgcg agcatcaggc ggcggtcgat
     4501 cttgtagacc ccggcgagca ccgcgtcgag cgccgcctcc agctcctcct gccggaactc
     4561 cgccagatag ccgaacgtgc cgtagttgac gccgagcacc ggcaccccgc ggtcgccgag
     4621 catgcgcgcc gcgccgatca tcgtcccgtc gccgccgagc acgacgatga gatcgacgct
     4681 atcgaccagc tcctcgtgct tcatcttctc gatggtgcag ccggactcgg cctcgaccgc
     4741 ggtgcggtcg atcttggggc cgccgacgag cgcgatgccg tggcgcgcgc accactcggc
     4801 gagccggcag atggtcttca gcgcttccgg ctggtgcggc ttgacgagga cgccgacgcg
     4861 gcgaatcgaa ggcttctcca tgtcgagaac gggtgcgggc ggaagagact ctctcggcgt
     4921 tcacgcgccc gcgcgcagac actcgcacga gcgcgcgaag cgaaccatcg aaatcgagcg
     4981 gaaaacttgc ggcggatgat agccgcgccc gccgcggggt gtcaacgaga agcagtaggc
     5041 agtaggcagt aggcagtagg cagcgggttg agtgaagcgg tggttgacgt tttaactcta
     5101 acgtctcaac agaggaaggt tttaactgcc tactgcctac cgcctaccgc ctactgtgtt
     5161 ccagtgtgcg cgtcgtgtgt tcgacgacgc tggcgggggg gcgttcgggc agggcgccgc
     5221 cgacgcgggc ggggccgagt tcgcccgcgg cgggcggggc tttgagctgc ggggtggtgg
     5281 gcaggcggcg cgcgccgccg agcagataag tccagagcca ggtcagcgag gcgacgccgc
     5341 cgaggatggt gaacgcgccg aaggtgacga tcatcttgaa cacgtccggc ggcaggccgc
     5401 tgccccgcat ctcgctgatg aaggcgagca gcaggccgag gcccatcacg acgagcagca
     5461 gcatcgagag gccgaccgcg caggccgtgc cggtcgtgat cgcgggccgc atctccggcg
     5521 ccgtcgtcaa ggccgccacg ccgcccaggt tgccgccgca gcgcttgcag tagttgaggt
     5581 caacgttcgc ctcagtcccg cagtgttgac agaacatgtc gaattctccg gaaggaattt
     5641 ccgattgtgg attatgggtt ttggattgga gcggcggcgg aagtcgaacg ccggacagca
     5701 gccttcaagc tgaagcagcg cgcaatccaa aatccaaaac tgaaatccga aatctacaac
     5761 cggcttacgc gcgcagcacg ggcaaagttc tgtttttgag ccggcgggcg cgcgccgcgt
     5821 tgccgcgctg atggtcgtgc gcgcctcgca cgccccacgc gcgcgcgagg cgcgcgagtt
     5881 cggcgccgtc cgaagacggg tcaacgagcg tgagccagag gggcgtcggc agccacgccc
     5941 acgccgcgcc gtagacttcg ttgttgagct gttcgacgcg ccgctcctcg cgccactgca
     6001 agtcgagcgg gggcacgtcc tcccagcggt cgtaacaggc gtgctggatg cacggcgcgg
     6061 gcttcgcgcg acggtggacg aggcagcccg cgcccggctc gaacagcggg cacgagtagg
     6121 tgcgcgcgaa cgtgtcgccc cccgcgcgcg cgaggccgta cctttcgacc gccgcgcgcg
     6181 cgcgcgcgta gacggcgcgc ctctcttctt ccgtcagccg cggcgtgcgc gccagcgtct
     6241 cgcggatggc gacggcttcg aggcgcgtga tgtggacgtt gacgaagtgc gcgtcggtgc
     6301 agcacacgcc cttcgtcggg cacgcgccgc agtcgcgcgc cgcgcgctcg aagttgagct
     6361 tgacgcggct ctggtacgcg gccttgctcc gctgcaaacg cgcgagcgcc gcctcttccg
     6421 tgagcttcct catgggttcg attcatccga acggcttcca gcggtacgag atgtcttcac
     6481 actcgatcag ccaaccaccg gcccgcgacc attcgatttc atgcaggacg tttccctgtt
     6541 cggataatcc gatctcgtca tacagccaat cccggtgccc gcgattatcg gcgggcaatg
     6601 cgaccgcggg caggtccaaa gaatagctgt agacgttcgt gtagacgagt tcgatctggc
     6661 cgtcgtggta agcggcaaga agtctgacgg cgatctcgac ggttcgatac tgttgcctct
     6721 ctccgtgcga cggctcacgg atcgttatcg cctccagcca cccgtcgtgc ggggatttgt
     6781 ggtccgtgaa atcgtagtgc cagggggccg tcgcgaattc gtaagcggaa cgcggcagac
     6841 gatcctttat ccgttgaatg taattcgaat aagcttccca cgcggcgttc cactcttcgt
     6901 cagagttccg tggtctgtct ttggtcagga tgaatgccat cttccgaatc cggcgggacg
     6961 acgcgcttaa ccccgcgact tctcgtacag ggcgaggaac tcgcggttgc cgtccgcgcc
     7021 ggtgatgggc gagtcggtga cgccgcggac gccgaggccg agcgcgcgcg cggcggcgtt
     7081 cacttcctcg acgacgcgcg cgtgccgcgc ggggtctcgg acgacgccgc ctttgccgac
     7141 ttcgccgcgc cccacttcga actgcggctt gatgaggacg acgaggtgcg cgccctgttc
     7201 gagcaagggg acgaccgccg gcatcacctt cgccgcggag atgaaggaga catccatcac
     7261 ggcgaggtcg aagcgctccg tgccgaagtc cgcggggcgg aggtggcgcg cgttgacgcc
     7321 ctcgcggact tcgacgcgcg gatccgtccg cagccgccag tcgatctgat tgtggccgac
     7381 atcgagggcg acgacgcggc gcgcgccgtg ctgcaagagg cagtcggtga acccgccggt
     7441 cgaggcgccc acgtcgagac acacgaggcc gcgcacgtcc aggccgaact cgcggagcgc
     7501 cttctccatc ttcagcccgc cgcgcccgac gtaacgcgcc gcagggtctt cgctcccgcg
     7561 cacgcgcagg ggcgcgtccg gcgccaccgt ctccgaaggc ttctcgacgc ggcgttcgcc
     7621 ggcgagcacc acgcccgcca tcaccagcgc ctgcgcccgc gtccgcgaag gcgccagccc
     7681 gcgctccacg agcagcttgt ccacgcgctc acgcttcacg gaaaaacttt aaggggaaaa
     7741 gggcaaaggg taaaggaaga aaaggcagaa gtcagaacgc agaacgatga at
//
LOCUS       DBNGOH010000113         2085 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1481953, whole genome shotgun sequence.
ACCESSION   DBNGOH010000113 DBNGOH010000000
VERSION     DBNGOH010000113.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2085)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2085)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2085
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1481953"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..537
                     /locus_tag="AC159P_02170"
     CDS             <1..537
                     /locus_tag="AC159P_02170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694288.1"
                     /translation="QSYTKGPAYSLALLLDASRRDDLQGVQTFINADQIAQGFIPQVI
                     DKLAAGGVQLPPNVQRGQLSAAIPQLIPRVSETMREEIARHMKAVAAEVGENTPTPLL
                     ALAVSRAAEIREDGDAATATFKSGERATELSLRRDGERWKVVTVKDDALATDIATRLA
                     ASLPPPAAPPPPRRRQGR"
     gene            701..1330
                     /locus_tag="AC159P_02175"
     CDS             701..1330
                     /locus_tag="AC159P_02175"
                     /inference="COORDINATES: protein motif:HMM:NF015786.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HIR3694289.1"
                     /translation="MEADSENASGAAKWDERYGRGEHATAEPSRLLVRAVELFEGEGR
                     AEGVAPPRALDIACGAGRHAVFLAARGFDVTAVDNSRVGVGLTRERARAAGVVVDARV
                     ADLERGEFAIEPGAYEVVCDFYYLQRDLFPRMRAGVKPGGLFVAAIHMVDDATGVKPM
                     NPDFLLGPGELRAEFEGWDVLHYRETEGHDPDAGRHTRRSAELIARKKQ"
     gene            1360..1953
                     /locus_tag="AC159P_02180"
     CDS             1360..1953
                     /locus_tag="AC159P_02180"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase [NAD(P)H] activity
                     [Evidence IEA];
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HIR3694290.1"
                     /translation="MAVKILGVSGSMRADSNSVRALRVVLAAAVARGAETRLLDLHEI
                     DLPVYRPQQAAETGGALASDAAVNWAEALVLASPDYHGSMSGAMKNFLDYFWEEFAGK
                     LFGYLCASHERGLTVMDQMRTAVRQCYGWSLPYGVAVHPKEDFDQAANDYTPAVTRRL
                     QMLARDMTVYGELITAQFRRDLEGDAADTFAARYRKK"
BASE COUNT          356 a          703 c          707 g          319 t
ORIGIN      
        1 cagagctaca cgaaggggcc ggcctacagc ctcgctctcc tgctcgacgc gtcgcggcgc
       61 gacgacctgc aaggcgtcca gacgttcatc aacgccgacc agatcgccca agggttcatc
      121 ccgcaggtca tcgacaagct cgccgcgggc ggcgtccagc tcccgcccaa cgtgcagcgc
      181 ggccagctct ccgccgccat cccgcagctc atcccgcgcg tcagcgagac gatgcgcgaa
      241 gagatcgcgc gccacatgaa ggcggtcgcc gccgaggtcg gcgagaacac gccgacgccg
      301 ctcctcgcgc tcgccgtctc gcgcgccgcc gagatcaggg aggacggcga cgccgcgacc
      361 gccacgttca agtcgggcga gcgcgccacc gagctgtcgc tccgccgcga cggcgagcgc
      421 tggaaggtcg tcacggtcaa agacgacgcg ctcgccaccg acatcgccac gcgcctcgcc
      481 gcgagcctcc cgccgcccgc cgcgccgccg ccgccgcgcc gccggcaggg aagatgagta
      541 aaagccgtga accgtgaacc ggaaaccgtg atgagttaag acagcatccc tctcgtcact
      601 cgtcactcgt cacagctttt atcttgtcac ggttcacggg tagtgggttt taacttgtca
      661 cggttcacgg ttcacggttt acggctcttg tccttagagt atggaagccg attcggaaaa
      721 tgcgtcgggc gcggcgaagt gggacgagcg gtacgggcgc ggcgagcacg cgaccgcgga
      781 gccgagccgc ctgctggtgc gcgcggtcga gctgttcgag ggagagggcc gggcggaggg
      841 tgttgcgccg ccgcgcgccc tcgacatcgc gtgcggcgcg ggccgccacg cggtgtttct
      901 ggccgcgcgc gggttcgacg tgacggcggt cgataactcg cgcgtcggcg tcgggctgac
      961 gcgcgagcga gcgcgggcgg cgggcgtagt cgtggacgcg cgcgtcgccg acctcgaacg
     1021 cggcgagttc gccatcgagc cgggtgctta cgaagtcgtc tgcgactttt attacctcca
     1081 gcgcgacctc ttcccgcgga tgcgcgcggg ggtgaagccg ggcgggctgt tcgtcgccgc
     1141 gattcacatg gttgacgacg caacgggcgt gaagccgatg aacccggact tcctgctcgg
     1201 ccccggcgag ctgcgcgccg agttcgaagg ctgggacgtg ttgcactacc gcgagacgga
     1261 ggggcacgac ccggacgccg gacgccacac gcgccgctcc gccgagctga tcgcgcgaaa
     1321 gaaacaatga acgcggaatc attgatggag aggtgagcgg tggccgttaa aattttaggt
     1381 gtgtccggca gcatgcgcgc cgactcgaac agcgtgcggg ccttgcgcgt cgtgctcgcg
     1441 gcggcggtgg cgcgcggggc cgagacgcgt ctgctcgacc tgcacgagat cgacctgccg
     1501 gtctaccgcc cgcagcaggc ggcggagacc ggcggcgcgc tcgcgtcgga cgcggcggtg
     1561 aactgggccg aggcactcgt tctggcctcg cccgactacc acggctcgat gagcggcgcg
     1621 atgaagaact tcctcgacta cttctgggag gagttcgcgg gcaagctctt cggctacctc
     1681 tgcgcctcgc acgagcgcgg gctgaccgtg atggatcaga tgcgcaccgc cgtgcgccag
     1741 tgctacggct ggagcctccc ttacggcgtc gccgtccacc cgaaggaaga tttcgaccag
     1801 gccgccaacg actacacgcc cgccgtcacg cgccgcctac agatgctcgc gcgcgacatg
     1861 accgtctacg gcgaactcat caccgcgcag ttccgccgcg acctcgaagg cgacgccgcc
     1921 gacacgttcg cggcacggta caggaagaag taggcagtag gcagttaaag caatggaaga
     1981 atatagaatc tcgaatttga atttcagtct ttcttaacag cctactgcct actgcctact
     2041 agttttatgc acgacgcaat catcatcggc gcaggcccgg cgggg
//
LOCUS       DBNGOH010000114         4054 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4415099, whole genome shotgun sequence.
ACCESSION   DBNGOH010000114 DBNGOH010000000
VERSION     DBNGOH010000114.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4054)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4054)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4054
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4415099"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            118..672
                     /locus_tag="AC159P_02185"
     CDS             118..672
                     /locus_tag="AC159P_02185"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR3694291.1"
                     /translation="MVDTAPETVSTAAAAPALHFDEAFTLHHRAVYRAAYALVRDTGL
                     AEDVTQEVFLKLYQNMETLQGDEHVKPWLLRVATNTALNTLRGRSRATARDEEFIKET
                     VGDGVTGLHADFERRVEIDEARRALEQVKEPMRSCILLKQQGLSYREIAETLSINEVN
                     VGSLIARGRKEFMRVYGKIGGRRQ"
     gene            669..1589
                     /locus_tag="AC159P_02190"
     CDS             669..1589
                     /locus_tag="AC159P_02190"
                     /inference="COORDINATES: protein motif:HMM:NF024881.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HIR3694292.1"
                     /translation="MKRCVDEGTLQAYLDGELSQESARETAAHVAACDACAAALAEAG
                     DETALFASAFAPDASLAVPTASLRARLNASVAQLEAGSAADTRESSGWSFGALVASLS
                     GLFAFTPQRAAALAGVAAVVVLFVSVFYIDRPGVEEGKSEQVAVVKQPDGGATPQPTA
                     SPDEPMASPDTPASVAGSKGATGSGAKPKSDAPRRKGQQFTPVPAPKLDRKFDRQFVP
                     GEKEYQSTIADLDKTIKSSGDSILKPSVRADYERNIAVLDRAIEETRAVALRNPKDRD
                     AARFLLSAYKSKVELMTTVADSAQVATLDR"
     gene            1632..2663
                     /locus_tag="AC159P_02195"
     CDS             1632..2663
                     /locus_tag="AC159P_02195"
                     /inference="COORDINATES: protein motif:HMM:NF024743.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HIR3694293.1"
                     /translation="MKFPALTILLALALPLAAGAQAQPREAAGEKGVAAQPGVERKIA
                     AAADVAVSLCMASGSVVVRGWDKGEVQARSDTAGRLDLREGKSGKGVEVIVSDYADDA
                     AGAGRCNSTSDVELDVPRGASVQIRVREGDVDIDGVAEARVESLNGDVDIRRVTRAVD
                     VSCLSGDVSLSDSKGRARLHSVSGSVEALHVSPAAAGDEFEAHSTSGDVSLERIGHAS
                     VKGATTSGSVRMSGPLAGSGTYEFATHSGDVTLELPADSSFRVNSRIIHTGEVITDFP
                     VRATLPAAGNVNEGGIPPPPQPPPPHGKGKVKPPKVPGHTGLVGVVGKGGAELKLTTF
                     SGTVYLKME"
     gene            2901..3779
                     /locus_tag="AC159P_02200"
     CDS             2901..3779
                     /locus_tag="AC159P_02200"
                     /inference="COORDINATES: protein motif:HMM:NF024743.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HIR3694294.1"
                     /translation="MRLVIGIAGAAAALVLSLSAQIVTASCGSAVDAGDETRPVAARP
                     EAEAAVAEPQSNEFRWRKALAAGRVVEVKGVNGNVRAEQAAGGEVEVVATKTSRRDDT
                     DTVRVEAVEHADGVTICAVYPSRDAAKPNVCAPGEGGRMNVQNNDVKVDFVVRVPSGV
                     RFAGRTVNGNVDATGMGADVDASTVNGNVKITTTGMARASTVNGSVTAAMGSAGWSDE
                     LSFSTVNGKLDLTFPASLSAEVNAETVNGDIMSDFPLTITGRYSKRKLNGTIGGGGRE
                     LHLRTVNGDVMIRRAS"
BASE COUNT          736 a         1296 c         1451 g          571 t
ORIGIN      
        1 cgggcggcgg aagttgaacg tccaacatca agcctcaagc atgagcagca cgctaatcca
       61 aaatctaaaa tccgaaatcc aaaatcgact taaatgagtg gtcaagcggt aagagccttg
      121 gttgacactg cgccagagac ggtctcgacg gcggcggcgg cgcccgcgct tcacttcgac
      181 gaagcgttca cgctgcacca ccgggccgtc taccgtgcgg cctacgcgct cgtgcgcgac
      241 acgggcctcg cggaagacgt gacgcaggag gtcttcctca agctctacca gaacatggag
      301 acgctgcaag gcgacgagca cgtcaagccg tggctgctgc gggtggcgac gaacacggcg
      361 ctcaacacgc tgaggggccg gagccgcgca acggcgcgcg acgaggagtt catcaaagag
      421 acggtgggcg acggcgtcac ggggctgcac gcggacttcg agcggcgcgt cgagatcgac
      481 gaggcgcggc gcgctttgga gcaggtgaag gagccgatgc gcagttgcat cctgctcaag
      541 cagcagggcc tctcgtaccg cgagatcgcg gagacgctgt cgatcaacga ggtcaacgtc
      601 gggagcctga tcgccagggg gcggaaagag tttatgaggg tctacgggaa gatcggaggg
      661 cgacggcaat gaagagatgt gtggacgaag ggacgctgca agcctacctc gacggcgaac
      721 tgtcgcaaga gtcggcgcgc gagacggcgg cccacgtcgc cgcctgcgac gcgtgcgccg
      781 cggcgctcgc cgaggcgggc gacgagacgg cccttttcgc ctcggccttc gcgcccgacg
      841 cgtcgctcgc cgtgccgacg gcctctttgc gcgcgcgcct caacgcgtcg gtcgcgcagc
      901 tcgaagcagg gagcgcggcg gacacgcgcg agtcgtcggg ctggagcttc ggcgcgctcg
      961 tcgcgtcgct gtcggggctg ttcgccttca cgccgcagcg cgcggcggcc ctcgcgggcg
     1021 tcgcggcggt cgtcgtcctc ttcgtcagcg tcttctacat cgaccgcccc ggcgttgaag
     1081 aagggaagag tgagcaagtc gcggtcgtca aacagccgga cggcggcgcg accccgcagc
     1141 cgacggcgtc gccggacgag ccgatggcgt cgccggatac gcctgcgagt gtcgcgggca
     1201 gcaagggcgc gacgggcagc ggcgcgaagc cgaagagcga cgcgccgcgg cgcaaggggc
     1261 agcagttcac cccggtgcct gcgcccaagt tggatcggaa gttcgaccgg cagttcgtgc
     1321 ccggcgagaa ggagtaccaa tcgaccatcg ccgacctcga taagacgatc aagtcgagcg
     1381 gcgactcgat cctcaagcct tcggtgcgcg ccgactacga gcgcaacatc gcggtgctcg
     1441 accgcgcgat tgaagagacg cgcgccgtcg cgctcaggaa cccgaaggac agggacgctg
     1501 cacggttcct gctctccgct tacaagagca aggtcgagct gatgaccacc gtggccgaca
     1561 gcgcgcaggt cgcgacgctc gaccgctgat cgaagcatct gcgttcgccg acgagattcg
     1621 gttttactta aatgaagttt ccggccttaa caattttact ggcgctggcg ttgccgctcg
     1681 cggcgggcgc gcaggcgcag ccgcgcgagg cggcgggcga gaagggcgtc gccgcgcagc
     1741 cgggcgtcga gcgcaagatc gccgccgcgg cggacgtggc cgtctcgctc tgcatggctt
     1801 cgggcagcgt ggtcgtgcgc ggctgggaca agggcgaggt gcaggcgcgc tcggacacgg
     1861 cgggccggct cgatttgcgg gaggggaagt cgggcaaggg cgtcgaggtg atcgtgtcgg
     1921 actacgccga cgacgcggcg ggcgcgggcc gctgcaactc gacgagcgat gtcgagcttg
     1981 acgtgccgcg cggcgccagc gtccagatca gggtgcgcga gggcgacgtg gacatcgacg
     2041 gcgtggccga ggcgcgcgtc gagagcttga acggcgacgt ggacatacgg cgcgtgacgc
     2101 gcgcggtgga cgtgtcgtgc ctgagcggcg acgtgtcgct ctcggactcg aaggggcgcg
     2161 cgcgcctcca ttcggtgagc ggctcggtgg aggcgctgca cgtctcgccc gccgcggcgg
     2221 gcgacgagtt cgaggcccac tcgacgagcg gcgacgtgag tctcgaacgc atcggccacg
     2281 cgagcgtcaa gggcgcgacc acgagcggca gcgtccgcat gtcaggcccg ctcgccggct
     2341 ccggcaccta cgagttcgcg acccactccg gcgacgtgac gctggagctt cccgccgact
     2401 cctccttccg cgtcaactcg cgcatcatcc acaccggcga ggtcatcacg gactttcccg
     2461 tgcgggccac gctgcccgcc gccggcaacg tcaacgaagg cggcatcccc ccgccgcctc
     2521 agcccccgcc gccgcacggc aagggcaagg tcaagccccc gaaggtgccg ggccacacgg
     2581 ggctggtcgg cgtcgtcggc aagggcggcg cggaactgaa gctgaccacc ttcagcggga
     2641 cggtctatct gaagatggaa taacgacacg aagacaaagg gcgcagaggt aaataggtta
     2701 agaggggaag agggaagacg agaggcacgc tgtctctcat cctccctctt aaccttttcg
     2761 cccttcttcc cctcgatttt ctgtcacaaa ccgcgcgggc gcggtgtttc cacattagaa
     2821 cagagggaag accgcgaaaa cgtctgccct ttcgcgcagg aaaggctttt acaatacttt
     2881 ttccggactg aggaggagac atgagactcg taatcggaat cgccggcgca gccgccgccc
     2941 tcgttctttc tttgtcggca cagatcgtca cggcttcctg cgggagcgcc gtggacgcgg
     3001 gcgacgaaac ccgcccggtc gcggcgcggc ccgaggcgga ggccgccgtc gccgagcctc
     3061 agtcgaacga gttccgctgg cgcaaggcgt tggcggcggg gcgcgtcgtc gaggtcaagg
     3121 gcgtgaacgg gaacgtgcgc gccgagcagg ccgcgggcgg cgaggtcgag gtcgtcgcca
     3181 cgaagacttc gcggcgcgac gacaccgaca cggtgcgcgt cgaggccgtc gagcacgccg
     3241 acggcgtgac catctgcgcc gtctacccga gccgcgacgc cgccaagccg aacgtctgcg
     3301 cgcccggcga gggcgggcgg atgaacgtcc agaacaacga cgtgaaggtg gacttcgtgg
     3361 tgcgcgtgcc ctcgggcgtg cgcttcgcgg gccgcaccgt gaacggcaac gtcgatgcga
     3421 cggggatggg cgccgacgtg gacgcctcga ccgtcaacgg caacgtcaag atcacgacca
     3481 cggggatggc gcgcgcctcg accgtcaacg gctcggtcac cgccgcgatg ggcagcgccg
     3541 gctggtcgga cgagctgtcg ttctcgaccg tcaacggcaa gctcgacctg accttccccg
     3601 cgagcttgag cgcggaggtc aacgccgaga ccgtcaacgg cgacatcatg agcgacttcc
     3661 cgctgaccat cacgggccgc tacagcaagc gcaaactgaa cggcaccatc ggcggcggcg
     3721 ggcgcgagct gcacctgcgc accgtcaacg gcgacgtgat gatccgccgc gcgtcgtaag
     3781 agcgctgtca gctattagct gtcagctatt agccgaacgg cagaggattg atgggagcgg
     3841 tgctgttatc aatcgtcaat caggaatccg aaagctaacg gctaacggct aagagctaac
     3901 agccggtaaa ggaacggcaa tgatgagaac gaggttgaag gcagagggta aaaggcgggc
     3961 gctggccgcg ctcttgctgg cggcgctgct gctgccgcag gcgttgggcg cgggccgcgc
     4021 gcagactggc gaagacgcgc tgctgacggc gacc
//
LOCUS       DBNGOH010000115         1506 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2949017, whole genome shotgun sequence.
ACCESSION   DBNGOH010000115 DBNGOH010000000
VERSION     DBNGOH010000115.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1506)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1506)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1506
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2949017"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..300
                     /locus_tag="AC159P_02205"
     CDS             <1..300
                     /locus_tag="AC159P_02205"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF051307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlgC family sugar phosphate
                     nucleotidyltransferase"
                     /protein_id="HIR3694295.1"
                     /translation="LETSLALMAAEGRNVELGEGSTIADGAEVSESVLWEGVRVGKGA
                     RVRRAILGAGVVVNENEVIENAAVVRAELVAGEERPAKGLAGEVRGANFVAPLPQ"
     gene            446..>1506
                     /locus_tag="AC159P_02210"
     CDS             446..>1506
                     /locus_tag="AC159P_02210"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HIR3694296.1"
                     /translation="MHHPSQITSNAFQTSARERIEGYVSRARGHAGGVVALTPDASTR
                     EYFRVPWAKKGSAIAAVYPEPFDPEIHPFLDLTRLFAEAKLPVPEILDVDPLNGIIVQ
                     EDFGDNQLRRFFETAAEDEREAHTERAIQLIADIQAATQLAFDRKSIASRLSFDEAKL
                     SWELDYFREHFFKSMRREELPRAEEAELRAELNDVSAELAARARVLCHRDFHSSNLMI
                     DRKGRLRMVDYQDARIGPASYDLVSLLLDRQTSLPSLAEIRERRLFFLEERHKRGLED
                     IDPDEFAREFRLMTVQRCLKAVGTFSYQTGVLGRGETYSQFINPMLRIVLQAAEWLNR
                     FPTLQTVIRERVGADKGLSA"
BASE COUNT          261 a          527 c          494 g          224 t
ORIGIN      
        1 cttgagacga gcctcgcgct gatggccgcg gaagggagga acgtcgagct gggcgaaggc
       61 tccacgatag ccgacggggc cgaagtgtcc gagagcgtct tgtgggaggg cgtgcgcgtc
      121 gggaagggcg cgcgcgtgcg gcgcgcgatc ctcggcgcgg gcgtcgtggt gaacgagaac
      181 gaagtaatcg agaacgcggc ggtggtgcgt gccgaactcg tcgcgggcga ggagcgcccg
      241 gcgaaggggc tggcgggcga ggtgcgcggc gcgaattttg tcgccccgct gccgcagtga
      301 tacaatccgc cgagcgctcc aaacaggctt aacttgcggg aagtgaggcg gaaaccggga
      361 gacgtggcag cggcccaggg ttggccccga gatcggcccc tgaggctgac aaaagtgacg
      421 ccgcgccgct cttccttacc gttcgatgca ccacccttcg cagatcacgt ccaacgcctt
      481 ccagacttcc gcgcgcgaaa gaatcgaggg ctacgtctcc cgcgcgcgcg ggcacgcggg
      541 cggcgtcgtg gccctcacgc cggacgcctc gacgcgcgaa tacttccgcg tcccctgggc
      601 gaagaagggg agcgccatcg ccgccgtcta ccccgagccg ttcgaccccg aaatccaccc
      661 cttcctcgac ctgacgcggc tcttcgcgga ggccaagctc cccgtccccg aaatcctcga
      721 cgttgacccg ctgaacggca tcatcgtgca ggaggacttc ggcgacaacc agctccgccg
      781 cttcttcgag accgccgccg aggacgagcg cgaggcgcac accgaacgcg ccatccagct
      841 catcgccgac atacaagccg cgacgcagct cgccttcgac cgcaagtcca tcgccagccg
      901 cctctccttc gacgaggcca agctctcctg ggagctggac tacttccgcg agcacttctt
      961 caagagcatg cgccgcgagg agctgccccg cgccgaagag gcagagctgc gcgccgagct
     1021 gaacgacgtg tcggcggagc tggcggcgcg cgcgcgcgtg ctctgccacc gcgacttcca
     1081 ctcgtcgaac ctgatgatcg accgcaaggg caggctccgc atggtggact atcaggacgc
     1141 gcgcatcggc ccggcgagct acgacctcgt ctcgctgctg ctcgaccggc agacctctct
     1201 cccttcgctc gcggagatac gcgagcgccg cctcttcttc ctcgaagagc ggcacaagcg
     1261 cggcctcgaa gacatcgacc cggacgagtt cgcgcgcgag ttccgtttga tgaccgtgca
     1321 gcgctgcctg aaggccgtcg gcaccttctc ttaccagacc ggcgtgctcg ggcgcggcga
     1381 gacctactcg cagttcatca acccgatgct ccgcatcgtc ctgcaagcgg ctgaatggct
     1441 caaccggttc ccgacgctcc agactgtcat ccgcgagcgc gtcggcgcgg ataaaggact
     1501 gagtgc
//
LOCUS       DBNGOH010000116         4349 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1116209, whole genome shotgun sequence.
ACCESSION   DBNGOH010000116 DBNGOH010000000
VERSION     DBNGOH010000116.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4349)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4349)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4349
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1116209"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..217
                     /locus_tag="AC159P_02215"
     CDS             <1..217
                     /locus_tag="AC159P_02215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694297.1"
                     /translation="GEGAEVVTVRGRVVSLPFGGWLARLRPDCARGARRSGGATRASR
                     QTSAQSAWARRRVLTKHALRARPFAAE"
     gene            complement(234..1586)
                     /gene="trmFO"
                     /locus_tag="AC159P_02220"
     CDS             complement(234..1586)
                     /gene="trmFO"
                     /locus_tag="AC159P_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013907480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HIR3694298.1"
                     /translation="MKEIVVIGGGLAGAEAAWQAAEGGARVRLCEMRPVRETPAHRTD
                     KLAEIVCSNSLKSDEPGTAPYLLKEELRRGGSLVLEVAMETRVPAGAALAVDRHRFAE
                     RMTERVAAHPRITLVREEATRIAQDAVTIIAAGPLCSDALAAEIVRLTGGGQLYFYDA
                     ISPIISADSINYDIAFRAARYGKGGDDYVNCPMDAAEYETFYQALVEAKSVPLQRFEE
                     TRWFEACLPIEELARRGVDTLRYGPMKPVGLRDPRTGREPYAAVQLRQENLMADAYNL
                     VGFQNHLRFGEQARVIRTIPGLEGAEFLQYGQIHRNTYINAPRVLAATMQMRELPDVF
                     FAGQISGVEGYVESVATGWLAGRNAALAARGLPLLEAPSKTATGALARYVSSADAANY
                     QPVNITFALLEPMAEEERRRFRKKRERHARQVELALKEWDDWLHTHRRREGAPLTATP
                     "
     gene            complement(1710..3365)
                     /locus_tag="AC159P_02225"
     CDS             complement(1710..3365)
                     /locus_tag="AC159P_02225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694299.1"
                     /translation="MRNTENTIKLVAGSPRKRARTTLTAVAVVGLLLALACAPALAQN
                     ASASGPPPARAEAKARKLPSPEKIVRDYLKAAGGKKRLGAIRDAVYEWEAEGTTGGGA
                     GRRVLFKAPSSRRVEVLAGDGVESVSAANARTAWRQARGAAVETLTGAEAQTNKLLAV
                     LEGGRLVGYERVKAMARTAGVAQVGDEQAYAVEFSTRDGGRTRYWFGAASKLLLRVED
                     AAGGATVTASDFRPVGGVLEAHRVTEEREGKVVGSYVLRAVRHNTGLADALFEPPSDA
                     ALDIAALLRELAGNQDEVDQRVNDYTYTRRVTERELNDRGEVRKEKTNVYEVYPVVGW
                     GRVQKLVSENGVALSPERAAKEEKRVAEELEKAEREAPKREEERRRKRAARAAKKARA
                     GGDAVDDDADDDVGIATFLRACELTGARRERFRERETVVFDFRPRAVLRARNRAESIV
                     SKLAGTIWVDPTDRQVMRLEARFVDGFKLGGGLAAVKSGSAFVFEQTRLADGVWLPRS
                     AQVNVSVRLLFIGKSVNETHEFSDYKRFSARTGEDKLDAPQKP"
     gene            complement(3555..3746)
                     /locus_tag="AC159P_02230"
     CDS             complement(3555..3746)
                     /locus_tag="AC159P_02230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694300.1"
                     /translation="MAETNEETLDDDVRAGGAVDEARDPSRDNEEHELEAEAESAAAM
                     DADVPQPPDASEATEGQPS"
     gene            complement(3864..>4349)
                     /locus_tag="AC159P_02235"
     CDS             complement(3864..>4349)
                     /locus_tag="AC159P_02235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694301.1"
                     /translation="TENIDRYQGRGGQCDAYQIQQDGPAPAPDDRSGGSLREGEWPEQ
                     GRPQDEAERIRRMMEEAREKVARKKAKLAGKDASKGAAGRGAAKKGAAKSAAKSSAKK
                     AAKKSAGKGAGGEKKSAAGKTAAKKGAAGKSAAKKSSAKKSSGKASKKGAGKKAGKAK
                     G"
BASE COUNT          559 a         1612 c         1413 g          765 t
ORIGIN      
        1 tggagagggc gcggaggtcg tgacggtgcg cggtcgcgtc gtctctttac ccttcggggg
       61 ttggctcgcg cggttgcggc ccgactgcgc gcggggcgcg aggaggagcg gcggcgcgac
      121 cagggccagc aggcagacga gcgcgcagag cgcgtgggcg cggcggcgcg tcctgacgaa
      181 gcacgcgctc cgcgcccgtc cgttcgccgc cgagtgaagc gcgcgggcgc gcgtcagggc
      241 gtcgccgtca gcggcgcgcc ctcccgcctc ctgtgtgtgt gaagccagtc gtcccattct
      301 ttcaaagcca gctcgacctg ccgcgcgtgc cgctcgcgct tcttgcggaa gcggcgccgc
      361 tcctcttccg ccatcggttc gaggagcgcg aaggtgatgt tgacgggctg gtaattcgcc
      421 gcgtccgccg aagagacgta gcgcgcgagc gcgcccgtcg ccgtcttcga gggcgcttcc
      481 agcagcggca gcccgcgcgc cgcgagtgcc gcgttgcgcc ccgcgagcca gcccgtcgcc
      541 acggactcga cgtagccctc gacgccgctg atctgcccgg cgaagaacac gtccggaagc
      601 tcgcgcatct gcatcgtcgc cgcgagcacg cgcggcgcgt tgatgtacgt gttgcggtga
      661 atctgcccgt actgcaagaa ctcggcccct tcgaggccgg ggatggtgcg gatgactcgc
      721 gcctgttcgc cgaaacgtag atgattctgg aagccgacga ggttgtaggc gtcggccatc
      781 aaattctcct gccggagctg cacggcggcg tacggctcgc ggcccgtccg cgggtctctc
      841 aaacccaccg gcttcatcgg cccgtagcgc agcgtgtcca cgccgcgccg cgccagctct
      901 tcgatgggca ggcacgcctc gaaccagcgc gtctcctcga agcgctggag cgggacgctc
      961 ttcgcttcga ccaacgcttg atagaaggtc tcgtactctg ccgcgtccat cgggcagttg
     1021 acgtagtcgt cgccgccctt gccgtagcgc gccgcgcgga aggcgatgtc gtagttgatc
     1081 gagtcggcgg agatgatggg cgagatggcg tcgtagaagt agagctgccc gccgcccgtc
     1141 agccgcacga tctccgcggc gagcgcgtcg gagcagagcg gccccgccgc gatgatcgtg
     1201 acggcgtctt gcgcgatgcg cgtcgcctcc tcgcggacga gcgtgatgcg cgggtgcgcc
     1261 gcgacgcgct ccgtcatccg ttccgcgaac cggtgcctat cgaccgccag cgccgcgccc
     1321 gcgggcacgc gcgtctccat cgccacttcg aggacgagcg agccgccgcg ccgcagctcc
     1381 tccttcaaca gatacggcgc cgtgcccggc tcgtccgact tcagagagtt cgagcagacg
     1441 atctccgcca gcttgtccgt ccggtgcgcg ggcgtctcgc gcacgggccg catctcgcac
     1501 agccgcacgc gcgcgccgcc ttccgccgcc tgccacgcag cctccgcgcc cgccaacccg
     1561 ccgccgatca ccacgatctc tttcatacgt cttcaatcgt actccacaag agccgtgaac
     1621 cgtgaaccgt gaaaagccgt gaaccgtgaa ccgtcaaccg tgacaagtga aaaactgctt
     1681 tctacctgtc acgctttacg gttcacggct cacggctttt gcggcgcgtc gagtttgtct
     1741 tcgccggtcc tggcgctgaa gcgcttgtag tcgctgaact cgtgcgtctc gttgacgctc
     1801 ttgccgatga agaggaggcg caccgagacg ttgacctgcg cggagcgcgg gagccagacg
     1861 ccgtcggcga ggcgcgtctg ctcgaagacg aaggccgagc cggacttgac cgcggcgagg
     1921 ccgccgccca gcttgaagcc gtccacgaaa cgcgcttcga gacgcatcac ctgccggtcg
     1981 gtcgggtcaa cccagatcgt cccggcgagc ttcgagacga tggactcggc gcggttgcgc
     2041 gcgcgaagga cggcgcgcgg gcggaagtcg aagacgaccg tctcgcgttc gcggaagcgc
     2101 tcgcgccgcg cgcccgtcag ctcgcacgcg cgcaggaatg tggcgattcc tacatcgtcg
     2161 tccgcgtcat cgtccactgc gtcgccgccc gcgcgcgcct tcttcgcggc gcgcgcggcc
     2221 cgcttgcgcc ggcgctcctc ctcgcgcttc ggcgcctcgc gctcggcctt ctccagctct
     2281 tcggcgacgc gcttctcctc cttcgccgcg cgctccggcg agagcgcgac gccgttctcg
     2341 ctgacgagct tctgcacgcg gccccagccg acgacggggt agacctcgta gacgttcgtc
     2401 ttctccttcc tgacctcgcc gcggtcgttc agctcgcgct ccgtgaccct tcgcgtgtag
     2461 gtgtagtcgt tgacgcgctg atcgacctcg tcctggttgc ccgccagctc gcggagcagc
     2521 gccgcgatgt cgagcgccgc gtccgacggc ggctcgaaga gcgcgtcggc gaggcccgtg
     2581 ttgtggcgca cggcgcggag gacatacgac ccgacgacct tcccttcgcg ctcctcggtc
     2641 acgcggtgcg cttcgaggac gccgcccacg gggcggaagt cggaggcggt gacggtcgcg
     2701 ccgcccgccg cgtcttcgac ccgcagcagc agcttgctcg cggccccgaa ccagtaacgc
     2761 gtgcgcccgc cgtccctcgt cgagaactcg accgcgtacg cctgctcgtc gcccacctgc
     2821 gcgacgcccg ccgtgcgcgc catggccttc accctttcgt aaccgacgag ccggccccct
     2881 tcgagcacgg ccagcagttt attcgtctgc gcctcggcgc cggtcaacgt ctcgaccgcc
     2941 gcgccgcgcg cctgccgcca agccgtgcgc gcgttggcgg cggagacgga ttcgacgccg
     3001 tcgcccgcca agacttcgac gcggcgcgac gagggcgcct tgaacaagac gcgcctgccc
     3061 gccccgccgc cggtcgtgcc ctccgcctcc cactcgtaca cggcgtccct gatcgcgccc
     3121 agacgcttct tgccgcccgc ggccttcagg taatcgcgca cgatcttctc cggcgagggg
     3181 agtttccgcg ccttcgcttc ggcgcgcgcg ggcggcggcc ccgaagccga agcattctgc
     3241 gcgagcgcgg gcgcgcaagc gagcgcgagc agcaggccga cgacggcgac cgccgttaac
     3301 gtcgtccgcg cgcgctttcg cggcgacccc gcgactagct ttatcgtgtt ctcagtgttt
     3361 cgcacggcgc gtggcgtggg ggtgttggag ttcatactct cgacgacgga ctgttgaagg
     3421 gtcggattcc tgaaccgccg tggagagagt gacagcaagt tcacacgtaa ccggcagaag
     3481 aaagggcgac gagttgacag ccgtcttcgc gtcgcccttt cgggtttgct tacgtgtgtt
     3541 agaggggagt tgtttcagga gggttggcct tcggtcgcct ccgaagcgtc gggcggctgc
     3601 ggcacgtccg cgtccatcgc cgccgccgac tccgcctcgg cctcaagctc gtgctcctcg
     3661 ttgtcccgcg aagggtctcg cgcctcgtcc acggcgccgc cggcacgcac atcatcatca
     3721 agcgtctctt cgttcgtctc cgccatcacc tctgctcctt gatatataaa atcgctgaat
     3781 ataaagccgg cggactcgcg gtcgcctgat cggagccgcg agtccgtacg ccgaccgtcc
     3841 tacgcgcacg cgcgaggcgg cgactaccct ttcgccttcc cggccttctt accggcgccc
     3901 ttcttcgacg ccttgccgct gctcttcttc gccgaagatt ttttcgccgc agacttcccg
     3961 gcggcgccct tcttcgcggc ggtcttcccg gcggcgctct tcttctcgcc gcccgcgccc
     4021 ttcccggcgg acttcttcgc ggctttcttc gccgaagatt tcgcggcgga cttagccgcg
     4081 cccttcttcg ccgcgcccct gccggcggcc cccttcgacg cgtcctttcc ggcgagcttc
     4141 gcctttttgc gggcgacctt ttcgcgcgcc tcctccatca tccgccggat gcgctcggcc
     4201 tcgtcctgcg ggcggccctg ctccggccac tccccttcgc gcaggctccc ccccgagcgg
     4261 tcgtccggcg ccggcgccgg cccgtcctgc tgtatctggt aggcgtcgca ctgcccgccc
     4321 cgcccctggt agcggtcgat gttttccgt
//
LOCUS       DBNGOH010000117         4386 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4415591, whole genome shotgun sequence.
ACCESSION   DBNGOH010000117 DBNGOH010000000
VERSION     DBNGOH010000117.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4386)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4386)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4386
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4415591"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            115..579
                     /locus_tag="AC159P_02240"
     CDS             115..579
                     /locus_tag="AC159P_02240"
                     /inference="COORDINATES: protein motif:HMM:NF012304.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase H family protein"
                     /protein_id="HIR3694302.1"
                     /translation="MKDVLIYCDGSSLGNGRGEPRAAAVALLGFRGVWRAFGEYLGEA
                     TNQQAEVAAAALGLEQLREPCRVTVLSDSRYVVETMSGRFRRKTNHEWWARLDRAAEG
                     HKISWEWIKGHAGHAAQEAVDLAARNIAAAGRADAEVLREAAAAVGDGDRRA"
     gene            722..2725
                     /locus_tag="AC159P_02245"
     CDS             722..2725
                     /locus_tag="AC159P_02245"
                     /inference="COORDINATES: protein motif:HMM:NF026220.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40/YVTN/BNR-like repeat-containing protein"
                     /protein_id="HIR3694303.1"
                     /translation="MTALILIHTPLRARLFAFVLVVVCALAAAAQPQPQQRGWLWQNP
                     LPRGNAIYAVRFAPDKLTGWAVGADGVILKTEDGGYRWREQRSPATVSLYGLYVKDRK
                     TAVAVGARGQALVTTNGGSRWKAHPTGVKDHLYGVAFAGAEGLHGWAVGSYGQIVKTA
                     DGGRTWKPQSSGTRKHLYAVAFADAKTGVAAGADGALLATEDGGASWADRTTASSLPF
                     TSVAFAGASKTAVAVGFGGTLMRTEDGGQTWAQLDAFTRTDLLSVFFMDETRGWVTGD
                     EGQVLSTANGGTVWFPVSVKTRPRLAAIHFVDEFTGWAAGRDGLILRTDDGGIEWRRL
                     TEGGRDDLADIFFLDGERGWAVGSRGRVLYTSSGGKRWTLRFSHTTARLNGVFFIDEA
                     KGWAVGERGTILRSTDGGLTWAAASTETKDDLFGVHFADEKNGLAVGARGRILRTDDG
                     GETWHLQVTGTQNWFESVAYADPKTAVAVGTNGTILRTEDGGETWRFVEANVKEWLYG
                     VAFADATRGYAVGERGVILRTDDGGKTWKGLETEARSNLYSIAVAARDEALAVGEQGR
                     VLQTKDGGETWDAVATVTSASLFGASYRGGSAAWVAGRGGTILKRTDALATVKIPKPR
                     IPPALRGGPPKLKPQDTEEALAPLLDDDIPRATPPKAKEPVKP"
     gene            2881..4098
                     /locus_tag="AC159P_02250"
     CDS             2881..4098
                     /locus_tag="AC159P_02250"
                     /inference="COORDINATES: protein motif:HMM:NF015722.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HIR3694304.1"
                     /translation="MSRRESEDSSAPKSAGETQPRREPEDEVDHARLAQLVAANYAPP
                     PAPILSRTLLDAPPAAVSASQDDAPPLMLHAPRSAVVPRPSILAAQAQSMPQTAAAPR
                     AAAPKRISLFLAGAALVLLALVSCALFGWMAMLKPTSGPPKVGVGDPPRNVVAPHDGP
                     DASVTQMFVPGGTFRMGRDDAPKTLINEYPAHAVAVQHFLMDRTEVTNAEYAKFVRET
                     GHRAPSNEAPREGEESRWEPWADGKPPAGQEQWPVRNVSLAEAVAFAAWRSKRDGVLY
                     RLPTEEEWEYAARSGGTFRLYPWGDEFFDDRAGVDAAFPRPVGSYPQGASREGVLDLI
                     GNVWEWTASDATAYPGNRDPEAAGKGMKVARGGSYQSSARGSAAVTATSRTYIPPETK
                     HPTLGFRLVRDGP"
     gene            4102..>4386
                     /locus_tag="AC159P_02255"
     CDS             4102..>4386
                     /locus_tag="AC159P_02255"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR3694305.1"
                     /translation="MPLDAGTTVGRYEVRSFIGAGGMGEVYLAEDTQLQRPIALKVLT
                     GAPAADEERLRRFALEARATSALNHPNILTIYEVGATDSLHFISTEYVAGL"
BASE COUNT          684 a         1509 c         1544 g          649 t
ORIGIN      
        1 ggcagttggc agatggcagt taaaaccctt gttgccgaag agtcatattt aacctttcaa
       61 catttctaac gcggagcggc tgcctgctgc tatctgccta ctgcctactg ttttatgaaa
      121 gacgtgctca tctattgcga cggttccagt ctcggcaacg ggcgcgggga gccgcgcgcg
      181 gcggccgtgg cgctcctggg gttccgcggc gtgtggcgcg cgttcggcga gtacctgggc
      241 gaggcgacga accagcaggc ggaggtcgcg gcggccgcgc tcgggctcga acagttgcgc
      301 gagccgtgcc gcgtgaccgt gttgagcgac tcgcgctacg tcgtcgagac gatgtcgggg
      361 cgcttccggc gcaagacgaa ccacgagtgg tgggcgcggc tcgaccgcgc cgccgaaggg
      421 cacaagatca gttgggagtg gatcaagggc catgcggggc acgccgcgca ggaggcggtt
      481 gacctcgccg cgcgcaacat cgccgccgcc ggtcgcgccg acgcagaggt gctgcgcgag
      541 gccgcggcgg cggtcggcga cggcgaccgc cgcgcgtgag cgctccggcg cgtgcgtgtt
      601 gggggtgtga ggcgttaaaa tcgctggcct attgcccgga gcacgcccga ccagtttggc
      661 ctccgagagt gaatcgcggt aagcgctgct gtctccccga ccgtttccgc cccgttgtgt
      721 aatgactgcc ctgattctca tccacacacc cttgcgcgcg cgcctcttcg cgttcgtgct
      781 cgtcgtcgtg tgcgcgttgg cggcggcggc gcagccgcaa ccgcagcagc gcgggtggct
      841 gtggcagaac cctctgccgc ggggcaacgc gatctacgcg gtgcgattcg cgccggacaa
      901 gttgacgggg tgggcggtcg gggccgacgg cgtgattctg aagacggagg acggcggcta
      961 ccggtggcgc gagcagcgct cgcccgcgac cgtctcgctc tacgggctgt acgtgaagga
     1021 taggaagacg gcggtcgcgg tgggcgcgcg cgggcaagct ctggtgacga cgaacggcgg
     1081 ctcgcgctgg aaggcgcacc cgaccggcgt caaagaccac ctctacggcg tggccttcgc
     1141 gggcgccgag ggcttgcacg gctgggccgt cggcagctac ggccagatcg tgaagaccgc
     1201 ggacgggggc cgtacgtgga agccgcaaag ctcgggcacg cgcaagcacc tctacgcggt
     1261 cgcgttcgcc gacgcgaaga cgggcgtcgc cgccggggcg gacggcgcgc tcctcgccac
     1321 cgaagacggc ggcgcgagtt gggccgaccg cacgaccgcc tccagcctcc cgttcacgag
     1381 cgtcgccttc gcgggcgcgt cgaagaccgc cgtcgcggtc ggcttcggcg ggacgctgat
     1441 gcgcaccgag gacggcggcc agacgtgggc gcagctcgac gccttcacgc gcaccgacct
     1501 gctctcggtc ttcttcatgg acgagacgcg cggctgggtg acgggcgacg aggggcaggt
     1561 gctctcgacc gcgaacggcg gcacggtctg gttccccgtc agcgtgaaga cgcgcccgcg
     1621 tctggccgcg atccatttcg tggacgagtt cacgggctgg gccgcgggcc gcgacggttt
     1681 gatcctgcgg acggacgacg gcggcatcga gtggcgtcgt ctgacggaag gcgggcgcga
     1741 cgatctggcg gacatcttct tcctcgacgg cgagcgcggc tgggccgtcg gctcgcgcgg
     1801 gcgcgtgctc tacacctctt cgggcggcaa gcgctggacg ctccgcttct cgcacaccac
     1861 ggcgaggctc aacggcgtct tcttcatcga cgaggctaag gggtgggcgg tcggcgagcg
     1921 gggcaccatc ctgcgctcga ccgacggcgg gctgacttgg gccgccgcct cgaccgagac
     1981 gaaggacgac ctgttcggcg tccatttcgc cgacgagaag aacggcctcg cggtcggcgc
     2041 gcgtggtcgc atcctgcgca cggacgacgg cggcgagacg tggcacctgc aagtcacggg
     2101 cacgcagaac tggttcgagt cggtcgccta cgccgacccg aagacggcgg tcgccgtcgg
     2161 cacgaacggc accatcctgc gcaccgagga cggcggcgag acgtggcgct tcgtcgaggc
     2221 gaacgtcaag gagtggctct acggcgtggc cttcgccgac gcgacgcgcg gctacgccgt
     2281 cggcgagcgc ggggtcatcc tccgcacgga cgacggcggc aagacctgga aggggttgga
     2341 gaccgaagcc cggagcaacc tctattcgat cgccgtcgcc gcccgcgacg aggcgctggc
     2401 cgtcggcgag caggggcgcg tgctgcaaac caaagacggc ggcgagacgt gggacgcggt
     2461 ggccaccgtc accagcgcct cgctcttcgg cgcctcctac cgcggcggct ccgcggcctg
     2521 ggtcgcgggc cgcggcggca ccatcctcaa acgcacggac gccctcgcca ccgtcaaaat
     2581 ccccaagccc cgcatccccc ccgccctgcg cggcggcccg cccaaactca agccgcagga
     2641 caccgaagag gccctcgccc ccctcctcga cgacgacatc ccccgcgcca ccccgcccaa
     2701 ggcgaaagag ccggtgaagc cctgaccgcg ggcacaactc atttcgaatt aacgcttgac
     2761 ttttcgggtc ggtgaagtag agtcgcccgt gctgcggccc ccgccgtggt tcctcgccaa
     2821 ccctctgagc ctgagtccat ctgttgcgcg ctcttcggtg ggtgcgccga cgggaggcca
     2881 atgtcccgac gagaatcgga agacagctcg gcaccgaaaa gcgcgggaga gacacagccg
     2941 cggcgggagc ctgaagacga ggttgaccac gcgcgcttag cgcagctcgt ggccgccaac
     3001 tatgccccgc cgcccgcccc cattctctcg cggacgctgc tcgacgcccc gcccgccgcc
     3061 gtctcggctt cgcaggacga cgcccctccg ttgatgctgc acgcgccgcg ctctgctgtt
     3121 gttccacgcc cctccatcct cgccgcacag gcacagtcca tgccgcagac cgccgccgca
     3181 ccgcgcgccg ccgcaccgaa gagaatttcc ttattcctcg cgggggcggc gctggttctg
     3241 cttgcgctgg taagctgcgc cctctttggt tggatggcga tgctgaaacc gacatcggga
     3301 ccgcccaagg tgggtgtggg tgatccccct cgcaacgtgg ttgcccctca cgacggcccg
     3361 gacgcttcgg tcacacagat gttcgttccc ggcggcacgt tccggatggg gcgggacgac
     3421 gccccgaaga cgttaatcaa cgaatacccg gctcacgcgg tcgcggtgca gcatttcctg
     3481 atggacagaa ccgaagtcac caatgccgag tacgcgaagt tcgtgcgcga gaccgggcac
     3541 cgcgccccga gtaacgaggc gccgcgtgaa ggggaagagt ctcggtggga gccgtgggcc
     3601 gacggcaaac cgcctgccgg tcaggagcag tggccggtca ggaacgtgtc actggcggaa
     3661 gccgtagcct tcgccgcgtg gcgctcgaaa cgcgacggcg tgctgtaccg cctgccgacc
     3721 gaagaggagt gggagtacgc cgcgaggagc ggcggcacgt tccggctgta tccgtggggc
     3781 gacgagtttt tcgacgaccg cgccggcgtg gacgcagctt ttccgaggcc ggtcggctcg
     3841 tacccgcagg gcgcctcccg cgagggcgtg ctcgacctga tcggcaacgt ctgggagtgg
     3901 acggcttcgg acgcaacggc ctaccccggc aaccgcgacc cggaagcggc aggaaagggg
     3961 atgaaggtgg ctcgcggcgg ctcgtaccaa agcagtgcgc gcggcagcgc ggcggtcact
     4021 gccacgtccc gcacctacat cccgcccgag accaaacacc ctacgctggg tttccgcctc
     4081 gtgcgcgacg gcccttaacc gatgcctctg gatgccggca caacggtagg ccgatacgaa
     4141 gtccgctcct tcatcggcgc gggcggcatg ggtgaggtct acctcgccga ggacacgcaa
     4201 ctccaacggc ccatcgcgtt gaaggtgctg accggcgcgc cggctgccga cgaggagcgc
     4261 ctgcgccgtt tcgcgctgga ggcgcgcgcc acctctgcgc tgaaccaccc taacatcctc
     4321 acgatctacg aggtcggcgc caccgactcg ctacacttca tctccaccga gtacgtcgcg
     4381 gggttg
//
LOCUS       DBNGOH010000118         1789 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1482577, whole genome shotgun sequence.
ACCESSION   DBNGOH010000118 DBNGOH010000000
VERSION     DBNGOH010000118.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1789)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1789)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1789
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1482577"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1789)
                     /locus_tag="AC159P_02260"
     CDS             complement(<1..>1789)
                     /locus_tag="AC159P_02260"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF012509.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005534 - galactose binding [Evidence
                     IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HIR3694306.1"
                     /translation="GSLCARFRELYGDRPVRLLRAPARINILGEHVDYVSYLPTYSLP
                     FGSREHDMLMLYGPSKSGRVRGASMHADYEPFDFSLDEGPAPGGGEGDEAEWLSYVFK
                     GETPAPHWGNYVKGSCFFARTKFGGRVARGFDFVVDSSIPPKGGASSSSALAVLAGAA
                     IREVNGVAYRPGELALDSSKAEWFVGTRGGSMDHLTICLARRSRAVRISYREGTTRLV
                     RLPPARLRWVTFFSHPADKGREIMLEYNERAAVSRLLIPAIIEGWKETDPRRHAAWGR
                     AVEALLSGTEGASDEAESLLRELPETITLAEVERDYARTYGECVEAFPALVEGRRGRA
                     LRVRAYALHHLGEVRRVSEAERLLNALSETDVRRAVSRLGELLNETHASLRNLYGVST
                     PEVERLVGIVLSDPGVRGARLMGGGFGGNVLALTAEENVSALTRRVQAEFYAPAGRDG
                     VGEGSVMISTPGEGLSGLGVESVWRGAVEEFNALGRGGTVYRRGVATMLDDVETDAVR
                     DEVWPVVVAAGKGSRARESGLGVPKPVAPVAGVPAILRVLRNVREGCGGSARAPVVIV
                     SPETEQAVREALAGEEVSYVVQRVPLGTGD"
BASE COUNT          282 a          678 c          584 g          245 t
ORIGIN      
        1 gtcgcccgtg ccgagcggca cgcgctgcac gacgtagctc acctcttcgc ccgccagcgc
       61 ctcgcgcacc gcctgctcgg tctcgggcga gacgatgacg acgggcgcgc gcgccgagcc
      121 gccgcagccc tccctgacgt tacgcagcac gcggagtatg gccggcacgc cggccaccgg
      181 cgccacgggc ttcggcacgc ctagccccga ctcccgcgcc ctcgacccct tcccagccgc
      241 gacgaccacg ggccacactt cgtcccgcac ggcgtccgtc tcgacgtcgt ccagcatcgt
      301 ggcgacgccc cgcctgtaga ccgtgccccc gcgcccgagc gcgttgaact cttcgaccgc
      361 accgcgccag accgattcga ctcccagccc cgagagccct tcgcccggcg tggagatcat
      421 cacggagcct tcgcccacgc cgtcccggcc ggcgggggcg taaaactcag cctgcaccct
      481 gcgtgtgagc gcggacacat tctcctccgc cgtcagcgcg aggacgttac ctccgaaccc
      541 gccgcccatc agccgcgcgc cgcggacgcc ggggtcggag aggacgatgc cgaccagcct
      601 ctccacctcc ggcgtgctca cgccgtagag gttccgcagg ctcgcgtgtg tctcgttgag
      661 cagttcgcct agtcgtgaca ccgcccgacg cacatccgtt tcggaaaggg cgttgaggag
      721 gcgctcggcc tccgaaacgc ggcggacttc accgaggtgg tgcagggcgt atgcgcgcac
      781 gcgcagcgcc cgcccgcgcc gcccttcgac gagcgccggg aaggcttcga cgcactcgcc
      841 gtaggtgcgc gcgtaatccc gctcgacctc tgccagcgtg atcgtctcgg ggagttcacg
      901 gagcagcgac tccgcctcgt cggacgcgcc ctcggtgccc gagagcagcg cctcgacggc
      961 gcgaccccac gccgcgtgac gccgcgggtc tgtctccttc cagccttcga tgatcgcggg
     1021 gatcagcagg cgcgagacgg ctgcgcgctc gttgtactcc agcattatct cccgcccctt
     1081 gtccgccggg tgggagaaga aggtgaccca gcgcaggcgc gccggcggga gccggacgag
     1141 gcgcgtcgtc ccctccctgt aagagatgcg cacggcgcgc gagcgcctcg cgaggcagat
     1201 cgtgaggtga tccatcgacc cgccgcgcgt gccgacgaac cactcggcct tcgacgaatc
     1261 cagggccaac tcgccgggcc tgtaagccac gccgttgacc tcgcggatcg ccgcaccggc
     1321 gagcaccgcc agcgccgaag aggatgaagc cccgcccttg ggcgggatgc tcgaatcgac
     1381 gacgaagtcg aagccccgcg ccacccgacc gccgaacttc gtgcgtgcga agaaacagga
     1441 acccttgacg tagttgcccc agtggggcgc cggcgtctcg cccttgaaga cgtaagagag
     1501 ccactccgct tcgtcaccct ctccgccccc aggcgcgggg ccttcgtcta gcgagaagtc
     1561 gaaaggttcg tagtcggcgt gcatggacgc gccccgcacc cgccccgact tcgaaggccc
     1621 gtacagcatc agcatgtcgt gctcgcggct gccgaagggg agcgaatagg tcgggaggta
     1681 cgacacgtag tcgacgtgct cgccgaggat gttgatgcgc gccggggcac gcagaaggcg
     1741 gacggggcgg tcgccgtaaa gctctcgaaa gcgggcgcac aacgaaccc
//
LOCUS       DBNGOH010000119         3202 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_566680, whole genome shotgun sequence.
ACCESSION   DBNGOH010000119 DBNGOH010000000
VERSION     DBNGOH010000119.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3202)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3202)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3202
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_566680"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..789
                     /locus_tag="AC159P_02265"
     CDS             <1..789
                     /locus_tag="AC159P_02265"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3694307.1"
                     /translation="TIVPPVWQRWWFVALAALTLGAAAYALYRLRVGRLIELERVRTR
                     IATDLHDDIGASLSKIAVLSDAAGQEVGSGSGRIAEPLAQIAGTSRELVDAMSDIVWA
                     VNPQRDHLSDLTHRMRRFASDLLEARDIEFTFHAPPDDRDLRLGADLRREVYLIFKEC
                     VNNLVRHAGCTRAEIEFGVAGRQLFVAVRDDGRGFDAAEAYRGGSGSGMGGHGLPGMR
                     RRAEALGGTFEIESANGGGTRVTLRVPTDGGRGRWRRGSYPKGR"
     gene            880..1536
                     /locus_tag="AC159P_02270"
     CDS             880..1536
                     /locus_tag="AC159P_02270"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR3694308.1"
                     /translation="METTNVNVIKVAVVEDMREIREALGRLFGNTAGFRCTGIYGSME
                     EALERVPRNLPDVLLCDIGLPGMDGIEGIGLLKERHPPLLVLMLTVYDDNERIFEALC
                     AGACGYLLKKTPAEKLVESVREAFEGGSPMSPEVARRVITLFREVRPPSRADYALTPH
                     EARLLKMLVEGHSYKTAAAELGVTVHTVKFHLRHVYEKLQVHTKSEAVAKALRHGLTR
                     "
     gene            1875..2405
                     /locus_tag="AC159P_02275"
     CDS             1875..2405
                     /locus_tag="AC159P_02275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694309.1"
                     /translation="MKKFGSSVFALAFFFALFAFAPAAGYGQKTSPTPASVNIVNTPS
                     VRDADNPARQPFQSELYLGGAASSLNFLEIAQVPAGKRLVIENVAVSGVTPTGQRVFA
                     GLITTMGNFIARHPLLVHAQGINENGEAEFVISQQARIYADPGSNVTIYVRRSSTSGA
                     SVVWHATISGYLVDVQ"
     gene            2467..2928
                     /locus_tag="AC159P_02280"
     CDS             2467..2928
                     /locus_tag="AC159P_02280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694310.1"
                     /translation="MKSRRGFMTLSMAAALVASVAAVSTSRAVTPRPTAVEYHSFGAI
                     SLVSGQGARVGVTNVQEATGLNFTIQFFDADGQVLKREEQYLKPGQTGSSEFVPDAPT
                     LSRRVATRAVVESAPDTPEVTGRQSLVNLEVFQVKTQATTVLAGPQLTLYR"
BASE COUNT          538 a         1112 c         1079 g          473 t
ORIGIN      
        1 accatcgtgc cgccggtctg gcagcgctgg tggttcgtgg ctctcgccgc gctcacgctc
       61 ggcgccgcgg cctacgcgct ctaccgcctc cgcgtcggcc gcctcatcga gctagagcgg
      121 gtgcggacgc gcatcgccac ggacctacac gacgacatcg gcgcgagcct ctcgaagatc
      181 gcagtcctga gcgacgccgc cggtcaggag gtcgggtcag gcagcggtcg gatcgccgag
      241 ccgctcgcgc agatcgccgg gacctcgcgc gagctcgtgg atgccatgag cgacatcgtc
      301 tgggcggtca acccgcagcg cgaccacctc tcggacctca cccaccggat gcgccgcttc
      361 gcctccgacc tgctagaggc gcgcgacatc gagttcacct tccacgcgcc gccggacgat
      421 cgcgacctgc gcctcggcgc cgacctgcgc cgcgaggtct acctgatctt caaggagtgc
      481 gtcaacaacc tcgtccgaca cgcgggctgc acgcgggccg agatcgagtt cggcgtagcg
      541 gggcgccagc tcttcgtcgc cgtccgcgac gacggcaggg gcttcgacgc cgccgaggcg
      601 taccgaggcg gctcgggctc cgggatgggc ggccacgggc tgccaggcat gcgccggcgc
      661 gctgaagccc tcgggggcac gttcgagatc gagtcggcga atggcggggg gacgcgcgtg
      721 acgctgcgcg ttccgaccga cggcggtcgc gggcgctggc ggcgcgggag ctacccgaaa
      781 gggcggtgaa ggcgccggcg gcgcgggagt aagctgacgc cgtcgtggta aacagggcgt
      841 cccgccgcgc cccggcggca gaccccaccg tgagaaaaga tggagacaac gaacgtgaac
      901 gtcatcaagg tggccgtcgt cgaggacatg cgcgagatac gcgaggccct cggcaggctg
      961 ttcggcaaca ccgcgggctt ccgctgcacg ggcatctacg ggtcgatgga ggaggcgttg
     1021 gaacgtgtcc cgcgcaacct gcccgacgtg ctgctgtgcg acatcgggct gcccggcatg
     1081 gacggcatcg aggggatcgg cctcctcaag gagcgccacc cgccgctcct cgtcttgatg
     1141 ttgactgtct atgacgacaa cgagcgcatt ttcgaggccc tgtgcgccgg ggcgtgcggc
     1201 tacctgctca agaagacccc ggcagagaag ctcgtggagt cggtgcgcga ggcgttcgag
     1261 ggcggctcgc cgatgtcgcc cgaggtggcg cgccgcgtca tcacactctt ccgcgaagtc
     1321 cgcccgccgt cgcgcgccga ctacgcgctg acgccgcacg aggcgcgcct gctgaagatg
     1381 ctggtcgagg ggcacagcta caagaccgcc gccgccgagc tgggcgtcac cgtccacacc
     1441 gtcaagttcc acctccgcca cgtctacgag aagctccagg tccacaccaa gtccgaggcc
     1501 gtcgccaagg ccctgcgcca cggcctgacg aggtaagccc cggtcgcgcc ctcgcacgac
     1561 gccgcgccgg cggcgtcaga cacctcacgg agcatactcg gccgaaccgc caaattcacg
     1621 cccgcccgcg cggggagttc gcgccgcgca cccaagccgg gcggggcggc ttccctccac
     1681 aatctccaac gcacccttct cctccggctg gcccgccgcg cagtgaccgc agaccggcct
     1741 ctaaaagctt actacccgaa tggctagtcc ttcggacccg acgcgccacg cgtttgctgt
     1801 gtgagaggca ccggcggtga agccggagcg cccttacaac cttggttcat tttcgacggg
     1861 ggaggagatt cgtcatgaaa aagttcggaa gcagcgtctt cgcgctcgcc ttcttcttcg
     1921 cactcttcgc cttcgccccg gccgccggct acgggcagaa gacttcgccc accccggcca
     1981 gcgtcaacat cgtcaacacg ccgagcgtgc gcgacgccga caacccggcc cggcagccgt
     2041 tccaaagcga actctacctg ggcggagcgg ccagcagctt aaacttcctg gaaatcgccc
     2101 aagtaccggc cggcaagcgg ctcgtcatcg agaacgtcgc ggtctcgggt gtcaccccga
     2161 cggggcagag ggtttttgcc ggcctcatca cgacgatggg gaacttcatc gcacgccacc
     2221 cgctgctcgt ccacgcgcag gggatcaacg agaacgggga ggccgagttc gtcatcagcc
     2281 agcaggcgcg catctacgcc gatcccggca gcaacgtcac catttacgtc aggcggagca
     2341 gcacctcggg agcatccgtg gtctggcacg cgaccatctc cggctacctc gtggacgtgc
     2401 agtagcccca cgcggacgcc ggtaattcta ttggccagca gcatcaggaa gagaaaggag
     2461 cttgtgatga agagcaggag agggttcatg acgctgagta tggcggccgc gctggtcgcc
     2521 tccgtcgcgg cggttagcac gagcagggcc gtgacgcccc gcccgacggc ggtcgaatac
     2581 cacagcttcg gcgcgatcag cctcgtctcg gggcagggcg cgcgtgtcgg cgtcaccaac
     2641 gtgcaggagg cgacggggct gaacttcacc attcagtttt tcgacgccga cgggcaggtg
     2701 ctcaagcgcg aagagcagta cctgaagccc ggccagaccg ggtcgagcga gttcgtcccg
     2761 gacgccccaa cgctgtcccg gcgggtcgcg acccgcgccg tcgtggagtc ggcacccgac
     2821 acgcccgaag tcaccgggcg gcagtccctc gtcaacttgg aggtgttcca ggtgaagacg
     2881 caagcgacca ccgtcctcgc cggcccgcag ttgacgctct accgctgacg gtcgtcggcc
     2941 gaaatatgaa gggggattcg acatggatcg gacgggattc gttatcgaca cgttcgcacc
     3001 cctcgcgggc cctttagctt tcacctccgc cgagggcggg aagtccggcg tcgcctccga
     3061 cttcggcaac ccttagcgcg cggcgggggg atatttcgga ggttgtcaga gtgacaaagc
     3121 gggcggggct tcgccgcgga aatgagtggc tcgcccgccg cggcagcggt tcggcaggtg
     3181 cgcgacgcgc ctaaggagga tc
//
LOCUS       DBNGOH010000120         1850 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2216370, whole genome shotgun sequence.
ACCESSION   DBNGOH010000120 DBNGOH010000000
VERSION     DBNGOH010000120.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1850)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1850)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1850
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2216370"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1850
                     /locus_tag="AC159P_02285"
     CDS             <1..>1850
                     /locus_tag="AC159P_02285"
                     /inference="COORDINATES: protein motif:HMM:NF025141.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT and vWA domain-containing protein"
                     /protein_id="HIR3694311.1"
                     /translation="LALAGAATANAQGVIVPGPCRRCPVPPRPIPLPRALPVKSVKIE
                     TKIAGQVATTHVEQVFRNDTDQTLEGTYFFPIPETASVSEFAIWDGDRRLVGEVRGRE
                     EARRIYDEIVRRQRDPGLLEYAGRDLFQASIYPIPPRSDKKLELTYTQVLRAEAGTVA
                     YRYPLGTGRNVAEIVGTVSGRVEIEGREPVRNIYSPSHEVSVARRDERHSRVSFESKA
                     GQEPRDFQLFYTLSDKDFGLTLLAHREPAKHGYFLLTISPKDEVSEAEYAAKDIVFVL
                     DTSGSMAEAGKMEKARAALLFGVRSLKDGDRFNVINFAGEEHLMASNMIWADAAGRAR
                     GEEFVKALRPVGGTNINDSLIAAMRQFLDGGERPRMLVFMTDGLPTVGVTNIRKIVEN
                     AREARAPGTRLFTFGVGYDVNTALLDKLAAENGGVAEYVEPKEDIEVKVSSFFNKINH
                     PVLTDLKLDTGGVETDLVYPRELPDLFRGSQVTIIGRYRNAADARNVTLRLTGRTGQR
                     ERSFAYENLSFPLTEGANEFLPRLWATRRVGWLMEQVRTNGEQKELTDEIVDLGTRYG
                     IVTPYTSYLALEEGMQVTDNATVATPPTARSISNLPLNNRAGGVGGGGGQG"
BASE COUNT          314 a          651 c          623 g          262 t
ORIGIN      
        1 ctggcgctcg cgggggcggc gaccgccaac gcgcagggcg tgatcgtgcc cggcccgtgc
       61 cgccgttgcc ccgtgccgcc gcggcccatc cctctgccgc gcgcgctgcc ggtcaagtcc
      121 gtcaaaatcg agacgaagat cgcgggccag gtggcgacca cgcacgtcga gcaggtcttc
      181 cgcaacgaca cagaccagac gctcgaaggc acctacttct tccccatccc cgagacggcc
      241 tcggtctcgg agttcgccat ctgggacggc gaccgcaggc tcgtcggaga ggtgcgcggg
      301 cgcgaggagg cgcggcgcat ctacgacgag atcgtgcggc ggcagcgcga ccccggcctg
      361 ctggaatacg cgggccgcga cctgtttcag gcgagcatct acccgatccc gccgcgctcg
      421 gacaagaagc tcgagctgac ctacacgcag gtcttgcggg ccgaggcggg cacggtcgcc
      481 taccgctacc cgctcggcac ggggcgcaac gtcgcggaga tcgtcggcac ggtctcgggc
      541 cgcgtcgaga tcgaggggcg cgaacccgtc cgcaacatct actcgccgag ccacgaggtc
      601 tcggtcgcgc gccgcgacga gcgccactcg cgcgtctctt tcgagtcgaa ggccgggcag
      661 gagccgcgcg acttccagct cttctacacg ctctcggaca aagacttcgg cctcacgctc
      721 ctcgcgcacc gcgagccggc gaagcacggc tacttcctct tgaccatctc gccgaaggac
      781 gaggtgagcg aggccgagta cgcggccaaa gacatcgtct tcgtgctcga cacctccggc
      841 tcgatggccg aggccgggaa gatggagaag gcgcgggcgg cgctgctctt cggcgtgcgg
      901 agtctgaagg acggcgaccg cttcaacgtc atcaacttcg cgggcgaaga gcacctcatg
      961 gcctcgaaca tgatctgggc cgacgcggcg ggccgcgcgc gcggcgagga gttcgtcaaa
     1021 gctctgcggc ccgttggcgg cacgaacatc aacgactctc tgatcgccgc gatgcgccag
     1081 ttcctcgacg gcggcgagcg cccgcgcatg ctcgtcttca tgaccgacgg ccttccgacc
     1141 gtcggcgtca ccaacatccg gaagatcgtc gagaacgcgc gcgaggcgcg ggcgcccggc
     1201 acgcggctct tcaccttcgg cgtcggctac gacgtgaaca cggccctttt ggacaagctc
     1261 gcggcggaga acggcggcgt cgccgagtac gtcgagccga aggaggacat cgaggtcaag
     1321 gtctcttcct tcttcaacaa gatcaaccac cccgtcctga ccgacctcaa gctcgacacg
     1381 ggcggcgtcg agacggacct cgtctacccg cgcgagctgc ccgacctgtt ccgcggctcg
     1441 caggtgacga tcatcggtcg ctaccgcaac gccgctgacg cgcgcaacgt cacgctgcgg
     1501 ctcaccggca gaaccggcca gcgcgagcgc tcgttcgcgt acgagaattt gagcttcccg
     1561 ctgaccgagg gcgcgaacga gttcctgccg cggctgtggg cgacgcggcg cgtcggctgg
     1621 ctgatggagc aggtgcgcac caacggcgag cagaaggagt tgaccgacga gatcgtagac
     1681 ctcggcacgc gctacggcat cgtcacgccc tacacctctt acctcgcgct cgaagagggc
     1741 atgcaggtga cggataacgc gacggtcgcc acgcctccga cggcgcgcag catctctaac
     1801 ctgccgctga acaaccgggc cgggggcgtg ggcggcggcg gcggtcaggg
//
LOCUS       DBNGOH010000121         2284 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4416074, whole genome shotgun sequence.
ACCESSION   DBNGOH010000121 DBNGOH010000000
VERSION     DBNGOH010000121.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2284)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2284)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2284
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4416074"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(260..1750)
                     /locus_tag="AC159P_02290"
     CDS             complement(260..1750)
                     /locus_tag="AC159P_02290"
                     /inference="COORDINATES: protein motif:HMM:NF025885.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HIR3694312.1"
                     /translation="MEEKKESDERGGERRHLLFVDDEDAARYGIRRALSNMNLDLSEA
                     GSVEAARPFIASKQPDLVLLDVNLPGLSGTEFLKELSASGEAAPLVILITAYGSERMA
                     VEAIRTGAYDYLSKPFEVDELRLVVKNALETVGLRRENRSLRKRIELEGERRGSLLGD
                     SEAILRVRSVIDKVAETDATVLVRGESGTGKELVARELHERSTHRRRGSFVAVNCAAL
                     PSELIESELFGHEKGAFTGAATRRRGKFEQADGGTLFLDEIGDMSSTVQAKLLRALEE
                     RRIERLGGSESIPVDVRIVSATHRPLEQEIERGNFRADLFYRLQVVTVEIAPLRERKE
                     DIPSLAEAFARQATERYGVPRRTISAAALRRLVEYPWPGNVRELRNTMERAMILAEGD
                     ELKVGDLPDEINGGARAEKTKDAPPSEGDALFVPFTSDFREDRREFERRYIARCLEES
                     GGNVTKAAAVLGMHRQSLQHKLRELGLARRYVAVGGEDEAPEKNDE"
     gene            complement(1788..>2284)
                     /locus_tag="AC159P_02295"
     CDS             complement(1788..>2284)
                     /locus_tag="AC159P_02295"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR3694313.1"
                     /translation="PHTESAHRAAELVGNIVRLFRAEAQARDIDITHDLVDERLELDG
                     ARAAAVRDAVSNLLHNALQATPDGGRIAVTGRREGASLLLAVCDSGAGVPPEQRDKIW
                     EPFYTTKQRGTGLGLAIVRKRIEEVGGTARLAAPGNGNGDGARFELVVPLREQTAHEA
                     ARQA"
BASE COUNT          331 a          783 c          754 g          416 t
ORIGIN      
        1 gtaagaaaat ttttgaccgg gggagtttag ccgctcagac cgcggacacg caaagagggt
       61 gttgggagtt aggtgttggt tttaagagct atagcggggt tcattcgtat gcgacttccg
      121 ccaaatgccc catcttacgc tcgtttctag cgaacacggc ggcaaactgc cagcatcagg
      181 tcgcatccga atgaccaccg ctgtaacgca cttgagagtt aataacccaa tacccagtac
      241 ccaaaaccca acaccctttt tactcgtcgt tcttctccgg cgcctcgtct tcgccgccga
      301 ccgcgacgta gcggcgcgcg agtcccagct cgcgcagctt gtgttggagc gactggcggt
      361 gcatgccgag gacggctgcc gccttcgtca cgttgccgcc cgactcttcg agacaacgcg
      421 cgatgtagcg gcgctcgaac tcgcgcctgt cctcgcggaa gtccgaggtg aaagggacga
      481 acagcgcgtc gccttccgat ggcggcgcgt ccttcgtctt ttcggcgcgc gcgccgccgt
      541 tgatttcgtc gggcaggtcg ccgaccttca actcgtcgcc ctctgcaaga atcatcgcgc
      601 gctccatcgt gttgcgcagc tcgcgcacgt tgcccggcca ggggtactcg accaggcgcc
      661 gcagcgccgc cgcggagatc gtgcggcgcg gcacgccgta gcgctcggtg gcctgccgcg
      721 cgaaggcttc ggcgagcgag gggatgtcct ccttgcgctc gcgcaagggg gcgatctcga
      781 ccgtcacgac ctgtaggcgg tagaagaggt ctgcgcggaa gttgccccgc tcgatctcct
      841 gttcgagcgg gcggtgcgtg gcgctcacga tgcgcacgtc cacggggatc gactccgagc
      901 cgccgagcct ctcgatgcgc cgctcttcga gcgcgcgcag gagcttcgcc tgcacggtcg
      961 aactcatgtc gccgatctcg tcgaggaaga gcgtgccgcc gtcggcctgc tcgaacttgc
     1021 cgcggcgccg cgtggccgcg cccgtgaagg cgcccttctc atggccgaac aactcggact
     1081 cgatcagctc cgaagggagc gcggcgcagt tgacggcgac gaagctgccg cgccgccggt
     1141 gtgtgctgcg ctcgtgcagc tcgcgcgcaa ccagctcctt gcccgtgccg ctttcgccgc
     1201 gcacgagcac ggtcgcgtcc gtctccgcca ccttgtcgat gacggagcgg acacgcaaga
     1261 tggcttcgga gtcgccgagc agagagccgc ggcgctcgcc ttcaagctcg atgcgtttac
     1321 gcaggctccg gttctcgcgc cgcaggccga cggtttcgag cgcgttcttg acgacgagcc
     1381 gcagctcgtc cacctcgaac ggcttcgaga ggtaatcgta ggcgcccgtc ctgatggcct
     1441 cgaccgccat ccgctcggag ccgtaggccg taatcagaat cacgagcggc gccgcctcgc
     1501 ccgacgccga cagctccttc aaaaactcgg tgccgctcag gccgggcagg ttcacgtcca
     1561 gcaggacgag gtcgggctgc ttcgaagcga tgaagggccg cgcggcctcg acgctgcccg
     1621 cctccgacag gtcgaggttc atgttcgaga gcgcgcgcct gatgccgtag cgcgccgcat
     1681 cttcgtcgtc cacgaagagc aagtgccgcc gctcgccgcc tctttcgtcg ctctcttttt
     1741 tctcttccat agtttttgtt caatggcacc cgtcggagcg ggctgtctca tgcttgtcgg
     1801 gccgcctcgt gcgcggtctg ttcccgcagc gggacgacga gttcgaagcg cgcgccgtcg
     1861 ccgttgccgt tgcccggcgc cgcgagccgc gccgtgccgc cgacctcttc gatgcgcttg
     1921 cggacgatgg cgaggccgag gcccgtgccc ctctgcttcg tcgtgtagaa cggctcccag
     1981 attttgtccc gctgttccgg cgggacgccc gcgccgctgt cgcagacggc gagcagcagc
     2041 gacgcgccct cgcgcctgcc ggtaacggcg atgcgaccgc cgtcgggcgt cgcctgtagc
     2101 gcgttgtgca gcaggttcga gaccgcgtcg cggacggcgg cggcgcgcgc gccgtccagc
     2161 tcgagccgtt cgtcgacgag gtcgtgcgtg atgtcgatgt cgcgcgcctg cgcctccgcg
     2221 cggaagaggc ggacgatgtt gccgacgagt tccgcggcac ggtgcgcgga ctcggtatgc
     2281 ggcg
//
LOCUS       DBNGOH010000122         4831 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4782549, whole genome shotgun sequence.
ACCESSION   DBNGOH010000122 DBNGOH010000000
VERSION     DBNGOH010000122.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4831)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4831)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4831
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4782549"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            71..1129
                     /gene="obgE"
                     /locus_tag="AC159P_02300"
     CDS             71..1129
                     /gene="obgE"
                     /locus_tag="AC159P_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HIR3694314.1"
                     /translation="MFIDRTKIKVRGGRGGNGVTAFRREKFVPRGGPSGGDGGRGGDV
                     WLEADSSLNTLLHLRYNPEHTAGRGLHGEGSNRSGREGADVVVRVPVGTQVYDLETGD
                     LYCDLAEDGQRWQAARGGRGGFGNAHFATSTNRAPRYHQEGSLGEERELQLELKLLAD
                     VGLIGFPNAGKSTLISTVSAAKPKIADYPFTTLEPHLGVVDLGDYRTFVVADIPGLIE
                     GAHAGAGLGDRFLRHVERTKLLLHLVDVSSISGRDPAGDYEIINRELAAYDPRLAERP
                     QIVVATKLDALDEPERLERLRERAARDGKEFLEISSATNRGVRELVQIVARRLAEIAA
                     SEDNARSREARSAGHDAL"
     gene            1229..1900
                     /gene="nadD"
                     /locus_tag="AC159P_02305"
     CDS             1229..1900
                     /gene="nadD"
                     /locus_tag="AC159P_02305"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF000840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HIR3694315.1"
                     /translation="MERRRRIALYGGTFDPVHFGHTALARRLLELFSLDEVLFIPAHV
                     APHKRDREVSPALHRYAMLALATQDEERFRVSTVELDAPESPYTVGTLERFVETTGCE
                     ARLFFVMGADSWQEITTWREWERVLTLTDIIVVTRPGYEVGTAHVTRAISERVVDTRG
                     RGSVEEALNEEGGTRIYLSDAVVMDVSATGVRRAARDGADLGEMVAPPVAEYIRKYGL
                     YQRGA"
     gene            1948..2442
                     /gene="rsfS"
                     /locus_tag="AC159P_02310"
     CDS             1948..2442
                     /gene="rsfS"
                     /locus_tag="AC159P_02310"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HIR3694316.1"
                     /translation="MGETEAAAADASAAEGRAAEQAQGAGQADEGFDERMLAALHAAS
                     EKKALELVALDLREVASFTDFFLLATGTNARQVQAISDAVEEQLKQRGTRPLRIEGYK
                     TAEWVLLDYGDFIFHVFEDKARRFYDLERLWRDASRVALPAQLAADAGGTGGAEDSSL
                     RSEK"
     gene            2439..3380
                     /locus_tag="AC159P_02315"
     CDS             2439..3380
                     /locus_tag="AC159P_02315"
                     /inference="COORDINATES: protein motif:HMM:NF012385.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 interaction domain-containing protein"
                     /protein_id="HIR3694317.1"
                     /translation="MIELVARSESMREALRLAGRVAATDANVLVTGESGVGKDALALY
                     VHAKSKRAAGPFVKIDCAALPGGLLEAELFGFERGAFTGATQAKPGRLEAAHKGTLVL
                     DEIAHLSWDAQAKLLRVIERREFERLGGRRTVRVDARLLALTNVDLDDAVRRRALRED
                     LFYRLNVVRIHVAPLRERGEDVGPLAERFLETFAAKHGRRGRGFTPGALDVLAHYEFP
                     GNARELANIVERAVIVSEGARVAEEDLPEAVRTAARAHSRRAKRLTLAELEAEHVRET
                     LAFAGGNKSEAARLLGISRKNLYEKLARYQEGEGGKG"
     gene            3492..3956
                     /locus_tag="AC159P_02320"
     CDS             3492..3956
                     /locus_tag="AC159P_02320"
                     /inference="COORDINATES: protein motif:HMM:NF014629.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HIR3694318.1"
                     /translation="MRLRLIWVGKTRDGHLRALVEEYLKRLSRFARCEVTEVREGAAA
                     DERACLEEESKRIAGALASDTFVVLLDVGGEQQWSSEELAARIEDWQSRSVKEVAFVV
                     GGHLGVAQEVRRRAAVRWSLSRLTLTHEMARVLLVEQLYRAYTITRGLPYQK"
     gene            4130..4483
                     /locus_tag="AC159P_02325"
     CDS             4130..4483
                     /locus_tag="AC159P_02325"
                     /inference="COORDINATES: protein motif:HMM:NF013427.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HIR3694319.1"
                     /translation="MRVYRDRLLERRESLFSQVTEAEMSSRERDLEATQDPADMAANA
                     YTKELLISMSANDRKLLELIDEALVRVEKGEYGECVNCGEPVSERRLDAVPWARYCLK
                     CQDLQERGLLNQDED"
     gene            4567..>4831
                     /locus_tag="AC159P_02330"
     CDS             4567..>4831
                     /locus_tag="AC159P_02330"
                     /inference="COORDINATES: protein motif:HMM:NF039185.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double zinc ribbon domain-containing protein"
                     /protein_id="HIR3694320.1"
                     /translation="MKPAKTLGALYDAWLALVYPRACAACGAESVEARADAPACAACW
                     DATRQFGEADTLCWKCGAPTRGDVAEGKRGEVRCRRCEAEAFTA"
BASE COUNT          824 a         1440 c         1830 g          737 t
ORIGIN      
        1 gaccgctgaa agctaacgcc cgaaagccaa gcgctaagag ctaaaagcta atcgctgaaa
       61 gctggaagct ttgtttattg atcgcaccaa aatcaaggtt cgcggggggc gcggcgggaa
      121 cggcgtgacg gcgttccgcc gcgagaagtt cgtgccgcgc ggcgggcctt cgggcggcga
      181 cggggggcgc ggcggcgacg tgtggctcga agcagactct tcgctgaaca cgctgctcca
      241 cctgcgctac aacccggagc acacggcggg gcgcgggctg cacggcgaag gctctaaccg
      301 ttcggggcgc gagggcgcgg acgtggtcgt gagggtgccg gtcgggactc aagtttacga
      361 cctggagacg ggcgacctgt actgcgacct cgccgaggac gggcagcgct ggcaggccgc
      421 gcgcggcggg cgcggcggct tcggcaacgc gcacttcgcc acctcgacga accgcgcgcc
      481 gcgctaccat caggagggaa gcctcggcga agagcgtgag ctacagcttg agctgaagct
      541 gctcgccgac gtggggctga tcggcttccc gaacgcgggc aagtcgactt tgatttcgac
      601 cgtctccgcc gcgaagccga agatcgcgga ctaccctttc acgacgcttg agccgcacct
      661 cggcgtggtt gacctcggcg actaccgcac gttcgtcgtc gcggacatcc ccggcctcat
      721 cgagggcgcg cacgctggcg cgggcctcgg cgaccgcttc ctgcgccacg tcgagcggac
      781 gaagctgctg ctccacctcg tcgatgtgtc ttcgatctcg ggccgcgacc ccgcaggaga
      841 ttacgagatc atcaaccgcg agctggcggc gtatgacccg cggctggcgg agagaccgca
      901 gatcgtggtg gcgacgaagc tggacgcgct cgacgagccg gagcggctgg agcgtctgcg
      961 cgagcgggcg gcgcgggacg ggaaggagtt tctggagatt tcgtcggcga cgaacagggg
     1021 cgtgcgcgag ctggtgcaaa tagtcgcgcg gcggctggcc gaaatcgccg cttcggagga
     1081 taatgcgcgg tcacgcgaag cgcgctcggc tgggcacgac gcgctgtgag gacggcgcgg
     1141 gtcgcgcgtt cgacttcgaa ggcggcgacg gtggtgcgtg ttcgaccgcg aaggtggcga
     1201 ggggttgcaa gggttgacgg ttcgaagcat ggagaggcga aggcgcatcg cgctctacgg
     1261 cgggacgttc gaccccgtgc acttcgggca cacggcgctg gcgcgtcggc tgcttgagct
     1321 gttctcgctc gacgaggttc ttttcatccc ggcgcacgtc gccccgcaca agcgcgaccg
     1381 cgaggtcagc ccggcgctcc accgctacgc gatgctggcg ctggcgacgc aagacgaaga
     1441 gcgcttccgc gtctcgacgg tcgaactcga cgcgccggag agtccgtaca cggtcggcac
     1501 gcttgagcgg ttcgtggaga ctacggggtg cgaggcgcgg ctcttcttcg tgatgggcgc
     1561 ggactcgtgg caggagatca cgacgtggcg cgagtgggag cgcgtgctga cgttgacgga
     1621 catcatcgtc gttacgcggc ccggctacga agtcggcacc gcgcacgtca cgcgagcgat
     1681 aagcgagcgg gtcgttgaca cgcgcgggcg cgggtcggtc gaagaggctt tgaacgaaga
     1741 gggcgggacg cgaatctacc tgagcgacgc ggtcgtgatg gacgtgtcgg cgacgggcgt
     1801 caggcgcgcg gcgcgcgacg gcgctgactt aggcgagatg gtggcgccgc ccgtggccga
     1861 atacatcagg aagtacggct tgtaccagag aggcgcatga gacaggattc tttgatgccg
     1921 gaacaaagca agaagcggac gaagagaatg ggcgagaccg aggcggcggc ggctgacgcg
     1981 agtgcggctg aaggccgtgc ggcggagcag gcgcaaggcg cggggcaggc ggacgagggc
     2041 ttcgacgagc gcatgctggc ggcgctgcac gcggcctccg agaagaaggc gctggaactc
     2101 gtcgcgctcg acctgcgcga ggttgccagc ttcacggact tcttcctgct cgccacgggc
     2161 accaatgcgc ggcaggtgca ggccatcagc gacgcggtcg aggagcagtt gaagcagcgc
     2221 ggcacgcggc ccctccgcat cgagggctac aagacggcgg agtgggtgtt gctcgactac
     2281 ggcgacttca tcttccacgt cttcgaggat aaggcgcggc gcttctacga cctcgaacgc
     2341 ctgtggcgcg atgcgtcgcg cgtcgcgctc ccggcacagc tcgccgccga cgcgggaggc
     2401 acgggcggcg ccgaagacag ttctttgaga agcgagaagt gatcgagctg gtcgcgcgtt
     2461 cggagtcgat gcgcgaggcg ctgcggctcg cgggccgcgt cgcggcgacg gacgccaacg
     2521 tgctcgtgac gggcgagtcg ggcgtaggga aagacgcgct cgcgctttac gtccacgcca
     2581 agtcgaagcg cgcggctggc ccgttcgtca agatcgactg cgcggcgcta cccggcggac
     2641 tgcttgaggc cgagctgttc ggcttcgagc gcggcgcctt caccggcgcg acgcaggcca
     2701 agcccggcag gctcgaagcg gcgcacaagg gcacgctcgt cctcgacgag atcgcgcatc
     2761 tttcgtggga cgcgcaggcg aagctcttgc gcgtgatcga gcggcgcgag ttcgagcgtt
     2821 tgggcggtcg ccgcacggtg cgggtggacg cgcggctgct ggcgctgacg aacgtcgatc
     2881 tcgacgacgc ggtgcgccgg cgggcgctgc gcgaagacct cttctaccgc ctgaacgtcg
     2941 ttcgcatcca cgtcgcgccc ttgcgcgagc gcggcgagga cgtggggccg ctcgcggaga
     3001 ggttcctcga aacgttcgcc gccaagcacg ggcgacgcgg gcgcgggttc acgccgggcg
     3061 ctttggacgt gctcgcgcac tacgagttcc ccggcaacgc gagggagctg gcgaacatcg
     3121 tcgagcgcgc ggtgatcgtc tcggagggcg cgcgggtcgc cgaggaagac ttgcccgagg
     3181 ccgtgcgcac ggcggcgcgc gcgcactcgc gccgcgcgaa gcgtttgacg ctggccgaac
     3241 tcgaagccga gcatgtccgc gagacgctcg ccttcgcggg cggcaacaag tccgaggccg
     3301 cgcggctgct cggcatcagc cgcaagaatt tgtacgagaa gctggcccgg tatcaggaag
     3361 gagaaggggg aaaggggtaa aaggtgaaaa ggggaagagg ggcaaaggga caagccgcgc
     3421 cgggacaaca cagccaacag ttcttccttt tccccctttc ccctcttccc ctttgacccc
     3481 ggtcgcggct catgcgtttg cgcctgatct gggtcgggaa gacgagggac gggcacctgc
     3541 gggcgctcgt cgaggagtat ttgaagaggc tgtcgcgttt cgcgcggtgc gaggtgacgg
     3601 aggtgcggga gggcgcggcg gcggacgagc gcgcgtgcct tgaggaggaa agcaaacgca
     3661 tcgcaggtgc actcgcatct gatactttcg tcgtgcttct ggacgtgggc ggcgagcagc
     3721 agtggagttc ggaagaactg gcggcgcgga tcgaggactg gcagtcgcga tccgtgaagg
     3781 aggtggcctt cgtcgtcggt gggcacctgg gggtcgcgca ggaggtgcgc aggcgcgcgg
     3841 cggtgcgctg gtcgctctcg cgcctgacgc tgacgcacga gatggcgcgc gtcctcctcg
     3901 tcgagcagct gtaccgtgcc tacacgatca cgcgcgggct gccataccag aagtgagggg
     3961 gtttttcgat tcccgttttt ggatttccga tcgaaggccg ttggctctca accgtccgtg
     4021 tttcgtctct gccgggagcc gaccgtcgaa atcggagaca atcgaaaatc cagaatcggg
     4081 aatcggaaat tagtatgagg gagtaaacca gaggatggat aagaaaaaaa tgagagtcta
     4141 ccgggacaga ttacttgagc gccgcgagtc gcttttcagc caggtgacgg aggccgagat
     4201 gtcgagccgc gagcgcgacc tcgaagccac gcaggacccc gccgacatgg cggccaacgc
     4261 ctacacgaaa gagctgttga tctcgatgtc ggcgaacgac cgcaagctgc tggagctgat
     4321 cgacgaggcg ctggtgcgcg tcgagaaggg cgagtacggc gagtgcgtca actgcggcga
     4381 gccggtctcg gagaggcggc tggacgcggt gccgtgggcg cggtactgtc tcaagtgcca
     4441 ggacttgcag gagcgcggac tcttgaatca ggacgaggat tagagcgtaa taggcggcat
     4501 catgttcggg cggccccgcg gctggagagg gttccagccg cggggctttt cgtttatcct
     4561 ctcggcgtga aaccggcgaa gacactcggc gcgctctacg acgcttggct cgcgctcgtc
     4621 tacccgcgcg cgtgcgccgc gtgcggggcg gagagcgtcg aggcgcgagc cgacgcccct
     4681 gcgtgcgcgg cctgctggga cgcgacgcgg cagttcggcg aagcggacac gctctgttgg
     4741 aagtgcgggg cgcccacgcg cggcgacgtg gcggaaggga agaggggcga ggtgcgttgc
     4801 cgccgttgcg aggcggaggc gttcacggcg g
//
LOCUS       DBNGOH010000123         2843 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2766661, whole genome shotgun sequence.
ACCESSION   DBNGOH010000123 DBNGOH010000000
VERSION     DBNGOH010000123.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2843)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2843)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2843
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2766661"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1308
                     /locus_tag="AC159P_02335"
     CDS             <1..1308
                     /locus_tag="AC159P_02335"
                     /inference="COORDINATES: protein motif:HMM:NF017044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HIR3694321.1"
                     /translation="GQRKLFAYQYTIDDFPNGLRLVTVPTDYPNLVSLYIVVTAGSRN
                     EVEAGKSGFAHFFEHMMFRGSQNYTPEQREAVFTRAGAETNAYTSDDRTVYHATFSKA
                     DLEEIMRVEADRFLRLKYTPAQYKTEALAVLGEYNKNSASPFFKMNEVLRETAFRKHT
                     YSHTTMGYLKDIEDMPNQYDYSLEFYRRFYRPEYTTIVIVGDVTRAAALDLTKKYWSD
                     WKRGDYKADIAEEPAQREARTAHIDWPSPTLPHLVVAFRGPAYSDEAKDKPALDLLAQ
                     IAFGENSELYQKLVLKEQKVDVLSPDFSNQIDPELFAVYARVKSPSDVNYVRDQILAA
                     FKRAAETEIDQKQLDATRSRMRYGFAIAMDSSEAIASALAPYVSLRRTPETINKLFDT
                     YTGVTPGDIRDAARKYFRDDSRTVVTLAPKGMNLAALGREVND"
     gene            1308..2819
                     /locus_tag="AC159P_02340"
     CDS             1308..2819
                     /locus_tag="AC159P_02340"
                     /inference="COORDINATES: protein motif:HMM:NF017044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HIR3694322.1"
                     /translation="MRRTTTLVLAAFFALLPFGASAGAQNNMTNKSAGVLLPNRSPLV
                     TFRVLFMTGAGEDPQGKEGVAALTAAMLAQGGTRDMTYEQIVEAMYPMATSLGWQIDK
                     EMTVFAGTTHVDNLDAYYRLVSAMLLDPGFRQEDFARLKADAVNFLKVSLREGNDEEL
                     GKEYLYNIIYAGHPYAHHNMGTLAALERLTLDDVRAFYKQNYTRANLVLGLAGGYPAD
                     FPKTVEASFEKLPAGTASKKKYAAPTVAPGMRVEIVKRETRATAFSLGFPIAVNRSHK
                     DWPALALVASYLGQHRSSVSHLYQRLREARGLNYGDYAYVEYFPRGMFQFTPDPNLGR
                     QQQIFQIWIRPVEPQNAHFTLRATLYEYDKLVRAGMSQADFDSIRDFLVKYGNVLTST
                     QDAQLGYALDSRYYGIPDFNTYMREQLSRLTLADVNAALRRHLTSDRMRVVVITKDAE
                     ALRAAILAGKPSPITYNSAKPPELVAEDKVIQDYKINVRPEQVVIVPVERVFQ"
BASE COUNT          556 a         1075 c          796 g          416 t
ORIGIN      
        1 ggccagcgca aactgttcgc gtaccagtac acgattgacg acttccccaa cggcctccgg
       61 ctcgtcaccg tgccgaccga ttaccccaac ctcgtctcgc tctacatcgt cgtcaccgcc
      121 ggctcgcgca acgaggtcga ggccggcaag agcggcttcg cgcacttctt cgagcacatg
      181 atgttccgcg gcagccaaaa ctacacgccc gaacagcgcg aggccgtctt cacgcgcgcg
      241 ggcgccgaga ccaacgccta cacctcggac gaccgcacgg tttatcacgc gaccttttcc
      301 aaagccgacc tcgaagagat catgcgggtc gaggccgacc gcttcctgcg tctgaagtac
      361 acgcccgcgc agtacaagac cgaggcgctc gccgtgctcg gcgagtacaa caagaacagc
      421 gccagcccct tcttcaagat gaacgaagtg ctgcgcgaga cggccttccg caagcacacc
      481 tactcgcaca ccacgatggg ctacctcaag gacatcgagg acatgcccaa ccagtacgac
      541 tacagccttg agttctaccg ccgcttctac cgaccggagt acaccacgat tgtcatcgtc
      601 ggcgacgtga cgcgcgccgc cgcgctcgac cttacgaaaa agtattggag cgactggaag
      661 cgcggcgact acaaggccga catcgcggag gagccggcgc agcgcgaggc gcgcaccgcg
      721 cacatcgact ggccctcgcc gacgctgccg cacctcgtcg tcgccttccg cggccccgcc
      781 tactccgacg aggcgaagga caagcccgcg ctcgacctgc tggcgcagat cgccttcggc
      841 gagaactccg agctgtacca gaagctcgtc ctcaaagagc agaaggtgga cgtgctctcg
      901 cccgacttct cgaaccagat cgaccccgag ctgttcgccg tctacgcgcg cgtcaaaagc
      961 ccgagcgacg tgaattacgt ccgcgaccag atactcgccg ccttcaagcg cgccgccgag
     1021 acggagatcg accagaaaca gctcgacgcc acgcgctcgc gcatgcgcta cggcttcgcc
     1081 atcgccatgg acagctccga ggccatcgcc tccgcgctcg ccccctacgt ctctctgcgc
     1141 cgcacgcccg agaccatcaa caagctcttc gatacctaca ccggcgtgac gcccggagac
     1201 atccgcgacg ccgcccgaaa gtatttccgc gacgacagcc gcaccgtggt cacgctcgcg
     1261 ccgaagggca tgaacctcgc cgccctcggc agggaggtga acgactgatg cgacgcacca
     1321 cgactctcgt cctcgccgcc ttcttcgccc tgctcccttt cggcgcctct gcgggcgcac
     1381 agaacaacat gacgaacaag tctgccggcg tgctcctgcc gaaccgttcc ccgctcgtca
     1441 ccttccgcgt cctcttcatg acgggcgcgg gcgaagaccc gcaggggaag gagggcgtcg
     1501 ccgccctcac cgccgcgatg ctcgcgcagg gcggcacgcg cgacatgacc tacgagcaga
     1561 tcgtcgaggc gatgtacccg atggccacgt cgctcggctg gcagatcgac aaggagatga
     1621 ccgtcttcgc gggcacgacg cacgtcgata acctagacgc ctattaccgc ctcgtctccg
     1681 cgatgctcct cgaccccggc ttccggcagg aagacttcgc gcggctcaag gccgacgccg
     1741 tcaacttcct caaggtctcc ctgcgcgagg gcaacgacga ggagctgggc aaggagtacc
     1801 tctacaacat catctacgcc ggccacccct acgcgcacca caacatgggc acgctcgccg
     1861 cgctcgaacg cctcacgctc gacgacgtgc gcgcgttcta taaacagaac tacacgcgcg
     1921 ccaacctcgt cctcggcctc gcgggcggct accccgcgga cttcccgaag accgtcgagg
     1981 cgagtttcga gaagctcccg gcgggcacgg cgtcgaagaa aaaatacgcg gcgccgacgg
     2041 tcgcgcccgg catgcgcgtc gagatcgtca agcgcgagac gcgcgccacc gccttttcgc
     2101 tcggcttccc catcgcggtc aaccgctcgc acaaggactg gcccgcgctc gcgctcgtcg
     2161 cctcctacct cggccagcac cgctcctccg tgagccacct ctaccagcgt ctgcgcgagg
     2221 cgcggggcct gaactacggc gactacgcct acgtcgaata cttcccgcgc ggcatgttcc
     2281 agttcacgcc cgaccccaac ctcgggcggc agcagcagat tttccaaatc tggatacggc
     2341 ccgtcgagcc gcagaacgcg cacttcacgc tgcgcgccac gctctacgaa tacgacaagc
     2401 tcgtccgcgc agggatgtcg caggcggact tcgactcgat ccgcgacttc ctcgtcaagt
     2461 acggcaacgt gctcacgagc acgcaggacg cgcagctcgg ctacgcgctc gacagccgct
     2521 actacggcat ccccgacttc aacacctaca tgcgcgagca gctctcgcgg ctcacgctcg
     2581 ccgacgtgaa cgccgccctc cggcgccacc tcacgagcga ccgcatgcgc gtcgtcgtca
     2641 tcaccaaaga cgccgaggcc ctgcgcgcgg ccatcctcgc gggcaagccc tcgcccatca
     2701 cctacaactc ggccaagccg cccgagctgg tcgccgaaga caaggtcata caggactaca
     2761 agatcaacgt cagacccgaa caggtcgtca tcgtcccggt cgaacgcgtc ttccagtaaa
     2821 gcgagcgggc agtcggcagc ggg
//
LOCUS       DBNGOH010000124         2458 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1483223, whole genome shotgun sequence.
ACCESSION   DBNGOH010000124 DBNGOH010000000
VERSION     DBNGOH010000124.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2458)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2458)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2458
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1483223"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..589
                     /locus_tag="AC159P_02345"
     CDS             <1..589
                     /locus_tag="AC159P_02345"
                     /inference="COORDINATES: protein motif:HMM:NF015338.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HIR3694323.1"
                     /translation="ALGMNYVWAAPPNARPKDGERKRNGEKEEEETGVAILSPYPLTD
                     VARIVLAHEGPNRRRRAAIGATVRVGDVSLRVYSVHAETRMPLAKKVEHWGAVLEDLK
                     RHPHVRHVVVLGDFNTIKGKDVRAARSLFTGAGFHTPFPDSEETFKVLFLDFKLDWIW
                     LRGLKAGAYGIDKEIGLSDHWPLWAKVKLDNASGS"
     gene            complement(678..>2458)
                     /locus_tag="AC159P_02350"
     CDS             complement(678..>2458)
                     /locus_tag="AC159P_02350"
                     /inference="COORDINATES: protein motif:HMM:NF014385.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HIR3694324.1"
                     /translation="TGQPAQQGQQPQTGAPSSEVQPPPGVRPPGRESVPAAAPDDLGL
                     DVPQVAPNFRTAPAPFPVLQRVGVDMSEQRALTLREAIELALSNNKDIEVARQNVRAA
                     EFDLQGARGVYDPRFLTNSYYERTETPAASFLSGSPSGAVTQSGFYSASSVQGLAPWF
                     GGGYRVDFVANRITTDNLFAALNPQFPSSLTFNYTQPILRGRQFDQNRRAIEIARKNL
                     SLTDAQFRQRAIETITGVQRAYWDLVFALRNLQVQRDAVRDARAQLEHNRRLVAEGVL
                     APIDVVAAEAQISGFEQSVYSALDDVGRAENNLKNLIAVNRREELWNKAIVPTDSVEL
                     QTPPVSLESAMESALRTRPEIQSADVATAINEIEQRFAREQTRPQVDLVASYGLVGLA
                     GSFNSSTVNPLTASNTQLRDRLNELSVINGLAPLPAPPVQAIPEVLVGGVGQSFANLA
                     ANRFTNFRVGVTVNLPFRNTTAEAQLGRTLVERDRIRTQREQLEQLIQVDVRNALQLV
                     RTAESRLRAAASARNASEQQYASEKRKLDAGQSTVFLVLERQTALTNARASELRAQTE
                     LNKAIAELQRATGNSLEQNQVEVRVR"
BASE COUNT          354 a          777 c          916 g          411 t
ORIGIN      
        1 ggcgctgggg atgaactacg tgtgggccgc gccgccgaac gcgcgaccga aggacggcga
       61 gcggaagcgc aacggcgaaa aggaagagga ggagacgggc gtcgccatcc tcagcccgta
      121 tccgctgacg gacgtggcgc ggatcgtgct cgcgcacgag gggccgaacc ggcggcggcg
      181 cgcggccatc ggcgccacgg tgcgcgtcgg cgacgtgtcc ttgcgcgtct actcggttca
      241 tgcagagacg cggatgccgc tcgcgaagaa ggtcgaacac tggggcgccg tgctcgaaga
      301 cctcaagcgc cacccgcacg tccggcacgt cgtcgtgctc ggcgacttca acaccatcaa
      361 gggcaaggac gtgcgcgccg cccgcagcct cttcaccggc gcgggcttcc acacgccctt
      421 ccccgacagc gaggagacgt tcaaggtgct cttcctcgac ttcaagctcg actggatttg
      481 gctgcgcggc ctcaaggcgg gcgcctacgg catcgacaaa gagatcggcc tctccgacca
      541 ctggccgctg tgggcgaagg tgaaactcga taacgcgagt gggagttaaa gaagtggcgg
      601 agaaggggtg acggggagat aaaacggggt gtcttatcgc cccgtcaccc cttctcccct
      661 cctccccttc tgcttattca gcgcacacgc acctcgacct gattctgttc gagcgagttg
      721 ccggtcgcgc gttgcagctc ggcgatggcc ttgtttagtt cggtctgtgc gcgcagctcg
      781 ctcgcgcggg cgttggtgag cgcggtctgg cgttcgagga cgaggaagac cgtgctctgg
      841 ccggcgtcga gcttgcgctt ttcagaggcg tactgctgct cggaggcgtt gcgcgccgag
      901 gccgccgcgc gcagccgcga ctcggcggtg cggacgagct gcaaggcgtt cctcacgtcc
      961 acctggatga gctgttcgag ctgttcgcgc tgggtgcgga tgcggtcgcg ctcgacgagc
     1021 gtgcgaccga gctgcgcctc ggcggtcgtg ttgcggaagg ggaggttgac ggtgacgccg
     1081 acgcggaagt tggtgaagcg gttcgcggcg aggttggcga acgactggcc gacgccgccg
     1141 acgagcactt cggggatggc ctgcacgggc ggcgcgggca gcggcgcgag gccgttgatg
     1201 accgacagct cgttgaggcg gtcgcgcagt tgcgtgttcg aggcggtcag cgggttgacg
     1261 gtcgagctgt tgaacgagcc ggcgaggccg accagcccgt aggaggcgac cagatcgacc
     1321 tgcgggcgcg tctgctcgcg cgcgaagcgc tgctcgatct cgttgatcgc ggtcgccacg
     1381 tccgccgact ggatttcggg gcgcgtcctt aatgccgact ccatcgcgct ttcgaggctg
     1441 acgggcggcg tttgcagctc gaccgagtcg gtcgggacga tggccttgtt ccacagctct
     1501 tcgcggcgat tcacggcgat caggttcttg aggttgttct cggcgcggcc cacgtcgtcg
     1561 agcgcgctgt agacgctctg ctcgaagccg gagatttgcg cctcggcggc gaccacgtcg
     1621 atgggcgcga gcacgccttc ggcgaccagc cgccggttgt gttcgagctg cgcgcgcgcg
     1681 tcgcgtacgg cgtcgcgctg cacttgcagg ttacggagcg cgaagaccaa gtcccagtag
     1741 gcgcgctgga cgccggtgat ggtctcgatg gcgcgctggc ggaactgcgc gtcggtcagg
     1801 ctgaggttct tgcgcgcgat ctcgatggcg cggcggttct ggtcgaattg gcgcccgcgc
     1861 aggatcggct gcgtgtagtt gaaggtgagc gaggacggga actgcgggtt gagcgcggcg
     1921 aacaggttgt cggtcgtgat gcggttggcc acgaagtcca cgcggtagcc gccgccgaac
     1981 cacggcgcga ggccctgcac gctcgacgcg ctgtagaagc ccgactgcgt gacggccccc
     2041 gaggggctgc ccgagaggaa gctcgcggcg ggcgtctccg tgcgctcgta gtagctgttg
     2101 gtcaggaagc gcgggtcgta gacgccgcgc gcgccctgca agtcgaactc cgccgcgcgc
     2161 acgttctgcc gcgccacctc gatgtccttg ttgttcgaga gcgccagctc gatcgcctcg
     2221 cgcaaggtga gcgcgcgctg ctcgctcatg tccacgccga cgcgctgcaa cacggggaag
     2281 ggcgcgggcg ccgtgcggaa gttgggcgcg acctgcggca cgtcgaggcc gaggtcgtcg
     2341 ggcgccgcag cgggcacgct ctcgcggccc ggcggtcgca cgcccggcgg cggctgcacc
     2401 tcggacgaag gcgcgccggt ctgcggctgt tgcccttgtt gcgcaggctg acccgtct
//
LOCUS       DBNGOH010000125         3191 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1667070, whole genome shotgun sequence.
ACCESSION   DBNGOH010000125 DBNGOH010000000
VERSION     DBNGOH010000125.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3191)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3191)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3191
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1667070"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..949
                     /locus_tag="AC159P_02355"
     CDS             <1..949
                     /locus_tag="AC159P_02355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HIR3694325.1"
                     /translation="AEMLPHVHEVDRNAVQIWFRFFPLALFDAFERTEDPARLAQQLE
                     MQGTFLLRDQIDSSHTFLYGHRFWPEVKREVVRLAESDEAAAGASLAGLMREVASRVA
                     AGRKVEPSLAGGIAAVALMTLRQVGLVEFRRTPGEVLIDAQHARRAPEQILRERARDD
                     AQGLLGFLRTTDKTWTIVWDENDEARRFKAMNMQEVASGAATDKRPWHERDPRCTVDE
                     GPIPVQCRSASCGTCWVGVLGGAEKLTPVAARERRMMPAFGYIETDDPRPLIRLSCQA
                     QATGAVSIVIPPWNGYFGKYLKKRNEAPDTEPETESRQA"
     gene            997..1554
                     /locus_tag="AC159P_02360"
     CDS             997..1554
                     /locus_tag="AC159P_02360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694326.1"
                     /translation="MTNAKPSESKGRDAGGPPPLARDAEGRVTPSSLADVIQWFLTYD
                     PRVAVVNFPAVEALYQWRRQEQLSHDPEAFAFERAEDRLAVGVAQALATHDTERALHA
                     WVGELLAALDDATKTNEAITDAYGLRPDEEASVVTEAEKIPSRRERDIYLTCCWLETL
                     CTAEVRVLGWVYQGLYGRPFHPDNF"
     gene            1588..1857
                     /locus_tag="AC159P_02365"
     CDS             1588..1857
                     /locus_tag="AC159P_02365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694327.1"
                     /translation="MAKEPTNYANTSQTVVVDANSFDFETLEQSNGNATVVRFRVENP
                     DVRAGDVLLVLSGSEIHFHGMIGLIEEGFGVATDRRGSLLPATVH"
     gene            2350..>3191
                     /locus_tag="AC159P_02370"
     CDS             2350..>3191
                     /locus_tag="AC159P_02370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694328.1"
                     /translation="MRFLKLTIVALFVAALALSTAFNTRTVEGRFRAEEAPTTDLNVL
                     TDDIFNGFGDKGIPPDECSNPPVANRSFEDNKFIFDERETPEDGLGPVYNDLGCGSCH
                     QNPESGGISQIRELRAGHLDAEGNFVNPPGGQSLIQLRALAAPIQERLDVAPLENIRT
                     FRTSLNVIGVGFVEAIANGTIISIRDNQPSSMRGTIINVPVIEANGTLRVGRFGWKNQ
                     HASLVSFSGDAYLNEMGITNPFDGNGGMQENNSLGRSVAAFDEVPEPEDDGADVEAFA
                     NFMRA"
BASE COUNT          547 a         1154 c         1020 g          470 t
ORIGIN      
        1 cgcggaaatg ctgccgcacg tccacgaggt tgaccgcaac gccgtccaaa tctggttccg
       61 cttcttcccg ctcgctctct tcgacgcctt cgagcgtacg gaagaccccg cgcggctcgc
      121 gcagcagctc gagatgcagg gcacgttcct gctgcgggat cagatcgact cgtcgcacac
      181 tttcctctac ggccaccgct tctggcccga ggtcaagcgc gaggtcgtcc gcctcgccga
      241 gtcggacgag gcggcggcgg gcgcgtcgct ggccggactg atgcgcgaag tcgcttcgcg
      301 cgtcgccgcc gggcgcaagg tcgagccgtc gctcgcgggc ggcatcgcgg cggtcgcgtt
      361 gatgacgctg aggcaggtcg gcctcgtgga gttccggcgc acgccgggcg aggtcttgat
      421 cgacgcgcag cacgcgcgcc gcgcgcccga acaaatcctg cgcgagcgcg cgcgcgacga
      481 cgcgcagggg ctgctcggct tcctccgcac gacggacaag acgtggacca tcgtgtggga
      541 cgagaacgac gaggcgcgcc gcttcaaggc gatgaacatg caggaggtcg cctcgggcgc
      601 ggccacagac aagcgcccgt ggcacgaacg cgacccgcgc tgcaccgtgg acgaggggcc
      661 gatccccgtc cagtgccgct cggcctcgtg cggcacgtgc tgggtcggcg tgctcggcgg
      721 cgccgagaag ctgacgccgg tcgccgcgcg cgagcggcgc atgatgcccg ccttcggcta
      781 catcgagacg gacgacccgc gaccgctcat ccgtctgtcc tgccaggcgc aggccaccgg
      841 cgccgtctcc atcgtcatcc cgccctggaa cggctacttc ggaaagtatc tgaagaagcg
      901 gaacgaggcg ccggacaccg agccggagac cgagagccgg caggcgtgag gcgcgctggc
      961 cgcggcgccg gaaacggaag tgcgaaactg actcttatga cgaacgcgaa gccgagtgaa
     1021 tcgaagggaa gggacgcggg cggcccgcct cccctcgcgc gcgatgcgga ggggcgcgtc
     1081 acgccttcgt cgctcgcgga cgtgatccag tggttcctga cttacgaccc gcgcgtggcc
     1141 gtggtgaact tccccgccgt cgaggcgctc taccagtggc ggcggcagga gcagttaagc
     1201 cacgaccccg aagccttcgc cttcgagcgg gccgaagacc gcctcgccgt cggcgtcgcg
     1261 caggcgctcg ccacgcacga cacggagcgc gcgctccacg cgtgggtcgg ggagctgctg
     1321 gccgcgctcg acgacgcgac gaagaccaac gaggccatca ccgacgccta cggcctgcgg
     1381 cccgacgaag aggcttcggt cgtcaccgag gccgagaaga ttccctcgcg gcgcgagcgc
     1441 gacatctacc tgacgtgctg ctggctcgaa acgctctgca cggcggaagt ccgcgtcctc
     1501 ggctgggtct atcaggggct gtacggcaga cctttccatc ccgataattt ttgataagat
     1561 gcgcgcacga gcgcgaggtt ttggaacatg gcaaaagaac cgactaatta cgcgaacact
     1621 tcgcagaccg tcgtggtcga tgcgaactcc ttcgacttcg agacgctgga acagtccaac
     1681 ggcaacgcca cggtcgtccg cttccgcgtc gagaacccgg acgtgcgcgc gggcgacgtg
     1741 ctgctcgtcc tgtcgggcag cgagattcat tttcacggca tgatcggcct catcgaggag
     1801 ggcttcggcg tggccaccga ccgccgcggc tcgctcctgc ccgcgaccgt ccattgagcg
     1861 gcgcgcgccg taaggaaaat gttgacaagc accgggaacg gcagtaggct tcacgcgctc
     1921 accggctggc gtttcctaat caacactgct tgcccctccc accgggattc gtccgccgcg
     1981 cacggcgctc tcaacggaaa gcaccgctaa cttttctccg tcccgttcga gcttgccccg
     2041 ctctctaaag tctgtcaggg ctgacggcgc gacgcggcgc ccgcaacctc gctcagggcc
     2101 gcgccgcgcc gcgcacctcg ccaactcgtc tcagactcag caggccgagt ccgcccgcct
     2161 cggcgcactc acgcgccgcc gccgaccgaa ctcgccgtcc accacttcca agtgtctgcc
     2221 agagggatac aggagggcga aactcctcaa ggacgtgccc gccgacgggc acgccgcgca
     2281 gggtctttta gagaccactt tccccgcccg ccaggccgca gcgccgcgcg gggtggtaga
     2341 ggaggccaca tgagattttt gaaactgacc atcgtcgcgc tcttcgtcgc ggcgctcgcg
     2401 ctctcgaccg ccttcaacac gcggacggtc gagggccggt tccgcgccga ggaggccccg
     2461 acgaccgacc tcaacgtcct gaccgacgat atcttcaacg gcttcggcga caaaggcatc
     2521 ccccccgacg agtgctcgaa cccgcccgtc gccaaccgct ctttcgagga caacaagttc
     2581 atcttcgacg agcgcgagac ccccgaagac ggcctcggcc cggtctacaa cgacctcggc
     2641 tgcggctcgt gccaccagaa ccccgagagc ggcggcatca gccagatcag ggagctgcgc
     2701 gccggacatc tcgacgccga gggcaacttc gtcaacccgc cgggcggcca gtcgctcatc
     2761 caactgcgcg cgctcgccgc gccgattcag gagcggctcg acgtcgcccc gctcgaaaac
     2821 atccgcacct tccgcacctc gctcaacgtc atcggcgtcg gcttcgtcga ggccatcgcc
     2881 aacggcacca tcatctccat ccgcgacaac cagccctcct cgatgcgcgg caccatcatc
     2941 aacgtccccg tcatcgaggc caacggcacg ctgcgcgtcg ggcgcttcgg ctggaagaac
     3001 cagcacgcca gcctcgtctc cttctccggc gacgcctacc tgaacgagat gggcatcacc
     3061 aacccgttcg acggcaacgg cggcatgcag gagaacaact ctttgggccg ctcggtcgcc
     3121 gccttcgacg aggtgcccga gcctgaagac gacggggccg acgtggaggc gttcgccaac
     3181 ttcatgcggg c
//
LOCUS       DBNGOH010000126         2993 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1667278, whole genome shotgun sequence.
ACCESSION   DBNGOH010000126 DBNGOH010000000
VERSION     DBNGOH010000126.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2993)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2993)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2993
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1667278"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..698
                     /locus_tag="AC159P_02375"
     CDS             <1..698
                     /locus_tag="AC159P_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008476482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3694329.1"
                     /translation="RPRELSGGERQRVALARAVVREPRAFLMDEPLSNLDAKLRVQTR
                     GELKRLQHRLRTTTVYVTHDQAEAMTLAHRVAVMRGGRLQQFDTPLDVYHRPANRFVA
                     GFVGNPAMNFVEGRIDAGARTFVNAGGAFVVRLSDAHLDATKGRERVTLGVRPEHVTV
                     ETSAREGRLAGSVYVTEQMGNETLVFLDTGGERLIARAGADFRAEPEAPAWARLEPDR
                     LHFFDADSGDAIK"
     gene            867..1691
                     /locus_tag="AC159P_02380"
     CDS             867..1691
                     /locus_tag="AC159P_02380"
                     /inference="COORDINATES: protein motif:HMM:NF013429.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HIR3694330.1"
                     /translation="MKLALNGATTMRASLVEDIEAASAAGFDCLEIWAAKLRKFFNYG
                     GTTAELKRLLEESRLAPYSINSIERVTFRDREGRAKLFAECEELCRVASEIGCPYVVV
                     VPSPLPAGMGPEAVVEESVQTLRELAGIAERHGVGLAFEFLGQTGCSVQTLAAADEIV
                     RRVGRASVGLVIDSFHFYAGGSKVSSIEALDPARLFIFHINDAEDLPRERLEDRHRLL
                     PGLGILPLGEIVAALRRIGYDKVASVEIFRPEYWERDPRQLARDAKAAVEGVLQAG"
     gene            1862..2821
                     /locus_tag="AC159P_02385"
     CDS             1862..2821
                     /locus_tag="AC159P_02385"
                     /inference="COORDINATES: protein motif:HMM:NF014893.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR3694331.1"
                     /translation="MDKIKVGIIGAGYIGGVHAGVLARDERVVLSAVHDVDEGRARRL
                     ASSSGARVAGDAEELIGGVDAVYVTTPNTLHTELAVAAARAGRHVFCEKPLATNMTDA
                     RAVLDAAEGGGGVFQVGHNRRFAPVYVTLKRMLSEGPSPHSAHVKMNRGELLKPEWVG
                     DPSVTGGFLYETTIHMFDMMRFLFGEVVRMHAMGSTHEYPEVDDFSVLLGFESGAHAT
                     LCSSSDASWLFPFERVEVFCHHRTIVTREMESLVESDGLEGHHVTHSTHQLSKEEKWG
                     YVQEDRAFIDSIIDGGPPLVTALDGYKSVELVDACYRQVRGEK"
BASE COUNT          507 a          946 c         1057 g          483 t
ORIGIN      
        1 gcaggccgcg cgagctgtcg ggcggcgagc ggcagcgtgt ggcgctcgcg cgcgcggtgg
       61 tgcgcgagcc gcgcgcgttt ttgatggacg agccgctgtc gaacctcgac gcgaagctgc
      121 gcgtgcagac gcggggcgag ctgaaacggc tccagcacag gctgcggacg acgaccgtct
      181 acgtcacgca cgatcaggcc gaggcgatga cgctcgcgca ccgcgtcgcc gtcatgcgcg
      241 gcggtcggct ccagcagttc gacacgcccc tcgacgtcta ccaccgtccc gccaaccgct
      301 tcgtcgcggg cttcgtcggc aaccccgcga tgaacttcgt cgaaggcaga atcgacgcgg
      361 gcgcgcgcac gttcgtcaat gccggcggcg ccttcgtcgt ccgcctgtcg gacgcgcacc
      421 tcgatgcgac gaaggggcgc gagcgcgtga cgctcggcgt gcggcccgaa cacgtcaccg
      481 tcgagacgag cgcgcgcgag ggccggctcg ccggctccgt ctacgtcacc gagcagatgg
      541 gcaacgagac tttggtcttt ctcgacacgg gcggcgagcg cctcatcgcg cgcgcgggcg
      601 cggacttccg cgccgaaccc gaagcgcccg cgtgggcgcg cctcgaacct gatagactgc
      661 acttcttcga cgccgattcc ggcgacgcga ttaagtgaaa cggggccggg tgtcgggggc
      721 cgggggccgg ggtaaaagct ttagctcgcc ggacagtccg gttgtcctaa ccacggcaag
      781 gttcggcgag cgttaattgg agcaagtttc aattgactgt agaacgctca aaaacccggt
      841 ccccggcccc ctccgaagga gagtttatga aactggcatt gaacggcgcg acgacgatga
      901 gggcgagcct tgtcgaagac atcgaagcgg cgtcggcggc ggggttcgac tgcctcgaaa
      961 tctgggcggc gaagctgcgg aaatttttca actacggcgg gacgaccgcc gagctgaagc
     1021 gactgttgga ggagagcagg ctcgcgccgt acagcatcaa ctccatcgag cgcgtcacgt
     1081 tcagggacag ggaggggcgc gcgaagctgt tcgccgagtg cgaggagttg tgccgcgtcg
     1141 cgtcggagat aggctgcccg tacgtggtcg tcgtgccgag tccgctgccg gcgggcatgg
     1201 ggccggaggc ggtcgtcgaa gagtcggtgc agacgctcag ggagctggcg ggcatcgcgg
     1261 agcggcacgg cgtcggcctc gcgttcgagt ttctgggaca gaccggttgc agcgtacaga
     1321 ctctcgcggc ggcggacgag atcgtgcgcc gcgtcgggcg cgcgagcgtc gggctggtga
     1381 tcgacagctt ccacttctac gcgggcggct cgaaggtctc gtccatcgaa gcgctcgacc
     1441 ccgcgcgcct cttcatcttc cacatcaacg acgccgaaga cctcccgcgc gagcggctgg
     1501 aagaccgcca ccgcctgctg cccggcctcg gcatcctgcc gctcggggag atcgtcgccg
     1561 cgctccgccg catcggctac gacaaggtcg cgagcgtcga aatcttccgc cccgaatact
     1621 gggagcgcga cccgcgccaa ctcgcgcgcg acgcgaaggc ggcggtcgaa ggtgtccttc
     1681 aggcgggatg aagctaaaga gcgaagggaa ggtttaacca caaagacacg aagacacaaa
     1741 gacaagacgg aaagcgtcaa gcaggttctt cttctcttct ctctatctct ttgtctgcct
     1801 ctgtgccttt ctttgtgccc ttcgtgtctt tgtggttaaa ccttcctctg atggggtgag
     1861 tatggacaaa atcaaagtgg gcatcatcgg tgcgggttac atcggcggcg tgcacgccgg
     1921 ggtgctcgcg cgcgacgagc gggtggtgct gtcggcggtg cacgacgttg acgaagggcg
     1981 cgcgcgtcgg ctggcgtcgt cgtcgggcgc gcgggtcgcg ggcgacgccg aggagctgat
     2041 cggcggcgtg gacgcggtct acgtgacgac gccgaacacg ctgcacacgg aactggccgt
     2101 cgcggcggcg cgcgcggggc gacacgtctt ctgcgagaag ccgctggcga cgaacatgac
     2161 ggacgcgcgg gccgtgctcg acgcggctga aggcggcggc ggcgtcttcc aggtcggtca
     2221 caaccgccgc ttcgcgcccg tctacgtgac gctcaagcgg atgctctccg aggggccgtc
     2281 gccgcattcg gcgcacgtga agatgaaccg cggcgagctg ctgaagccgg agtgggtcgg
     2341 cgacccgtcg gtgacgggcg gctttcttta cgagacgacc atccacatgt tcgacatgat
     2401 gcgcttcctc ttcggcgaag tcgtgcggat gcacgcgatg ggttcgacgc acgagtaccc
     2461 ggaggtggac gacttctcgg tgctgctcgg cttcgagtcg ggcgcgcacg cgacgctctg
     2521 ctcctcttcg gacgcgagct ggctcttccc cttcgagcgc gtcgaggtct tctgccacca
     2581 ccgcaccatc gtcacgcgcg agatggaatc gctcgtcgag agcgacggcc tcgaagggca
     2641 tcacgtcaca cactcgacgc accaactgtc gaaggaagag aagtggggct acgtgcagga
     2701 agaccgcgcc ttcatcgact cgatcatcga cggcgggccg ccgctcgtca cggcgctcga
     2761 cggctacaag tcggtcgagc tggtggacgc ctgctaccgg caagtgcgcg gagagaagta
     2821 gcgggtggca ggcagctaag acggaagaag cgagaggctt gggaggtaaa gtcccaagcc
     2881 tctcccttct gcgccgcgta gcggcggctg tgtgtagccc ggcgtttcaa cgccggggca
     2941 accgtgccgc gagcgacccg cgtcgcgtca gcgacgcctg aatagtcaac gcg
//
LOCUS       DBNGOH010000127         1992 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2033843, whole genome shotgun sequence.
ACCESSION   DBNGOH010000127 DBNGOH010000000
VERSION     DBNGOH010000127.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1992)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1992)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1992
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2033843"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..540
                     /locus_tag="AC159P_02390"
     CDS             <1..540
                     /locus_tag="AC159P_02390"
                     /inference="COORDINATES: protein motif:HMM:NF051698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HIR3694332.1"
                     /translation="FNYGPATFDRRHIFVATYTYKVTAFKGLENSFAKALLDGYEISG
                     ITRLQSGPYLTVTGNTSIGGRRADYLGGDVLIPNGERNARNWINRAAFAPAPDTRRGT
                     AGVGIVQAPGSQTWDFSVRKRFALTERFKMQLQADIFNAFNRANFRDLEVNLANAAFG
                     TVGAAGPARNIQLGLKLTF"
     gene            653..>1992
                     /locus_tag="AC159P_02395"
     CDS             653..>1992
                     /locus_tag="AC159P_02395"
                     /inference="COORDINATES: protein motif:HMM:NF027479.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4861 family protein"
                     /protein_id="HIR3694333.1"
                     /translation="MRAKRLVVLLLPCLLLAAAAQQTWAAPRLKVIKLAVTNPSEQTR
                     AGENVVVSVADLRRVAPDFKAAAFVVTVSDAATLEEDARAIQATEVPSQADDLDGDRK
                     LDEIAFQIDLKPRETRVVSIAYGDAAAIQRLRADYPQRTDARFASKFEGVGWESEATA
                     WRIYFDRRNAIDLYGKRRPGLYLEMFGQPEYDYHEESPFGRDIYKIGDALGLGAVGAL
                     VDGKAVRVSDVKERAWRIVSAGPVRAVVELTYKGWNVGGREVNLTSRMTQWAGKRGFE
                     HRIRAEGAEGLTIVTGLPRKAGLSEQKGEPTDETPVLWRATWGRQVLKTGATAVESLP
                     DQNLGLAVITQGRGGKIITDDALNLLLQPELRGGEARWYVYGVWDQEQSELLKVTAKS
                     AFEKHRNGSMVLPSKGVTTEEGFLKLVRDAGLRMSQPVRVEVVSKAAAPQSAPP"
BASE COUNT          347 a          690 c          682 g          273 t
ORIGIN      
        1 ttcaactacg gcccggccac cttcgaccgc cgccacatct tcgtcgccac ctacacctac
       61 aaggtgacgg ccttcaaagg tctggagaac agtttcgcca aagcgctgct cgacggctac
      121 gagatcagcg gcatcacgcg tttgcagtcg gggccttact taaccgtcac ggggaacacc
      181 tccatcggcg ggcgccgcgc cgactacctc ggcggcgacg tgctcatccc gaacggcgaa
      241 cgcaacgcgc gcaactggat caaccgggcg gcgttcgccc ccgcgcccga cacgcgccgc
      301 ggcacggcgg gggtcggcat cgtgcaggcg ccggggtcgc agacgtggga cttctccgtc
      361 cgcaagcgtt tcgcgctcac cgagcgcttc aagatgcagc ttcaggccga catcttcaac
      421 gccttcaacc gcgccaactt ccgcgacctc gaagtcaacc tcgccaacgc ggccttcggg
      481 acggtcggcg cagcggggcc ggcgcgcaac atccagctcg gcctgaaact gactttctga
      541 acaaccggga acatcgtgtg ctgtcgcccc gcgttggcgg cgcggggccg cacgcgaaga
      601 gggggcgcgt accggcccgc gcccctctac ctttccacgc cgaagaaaaa tcatgagagc
      661 caaacgactc gtcgtgctgc tgctgccgtg tctgctgctg gcggcggcgg cgcaacagac
      721 ctgggccgcg ccgcgcttga aggtcatcaa gctcgcggtg acgaacccct cggagcagac
      781 gcgcgcgggc gagaacgtcg tcgtcagcgt cgccgacctc cggcgcgtcg cgcccgactt
      841 caaggccgcg gccttcgtcg tcaccgtctc cgacgccgcg acgctggaag aggacgcgcg
      901 cgccattcag gcgacggaag tgccgtcgca ggcggacgac ctcgacggcg accggaagct
      961 cgacgagatc gctttccaga tcgacctgaa gccgcgcgag acgcgcgtcg tctcgatcgc
     1021 ctacggcgac gcggcggcga ttcaacgcct gcgcgcggac tacccgcagc gcaccgacgc
     1081 ccggttcgcg tcgaagttcg aaggcgtggg ctgggagtcg gaggcgaccg cgtggcgcat
     1141 ctacttcgac cggcgcaacg ccatcgacct ctacggcaag cggcggcccg gcctctatct
     1201 ggagatgttc ggccagcccg aatacgacta ccacgaggag tcgccgttcg ggcgcgacat
     1261 ctacaagatc ggcgacgcgc tagggctagg cgcggtcggc gccctcgtgg acggcaaggc
     1321 cgtccgcgtc tcggacgtga aggagcgcgc gtggcgcatc gtcagcgcgg ggccggtgcg
     1381 cgccgtcgtc gagctgacct acaagggctg gaacgtcggc gggcgcgagg tcaacctcac
     1441 gagccgcatg acgcagtggg cgggcaagcg cggcttcgag caccgcatca gggccgaggg
     1501 cgccgaaggg ctgaccatcg tgacggggct gccgcgcaaa gccggcttga gcgagcagaa
     1561 aggcgagccg acggacgaga cgcccgtgct gtggcgcgcg acgtggggcc gtcaggtctt
     1621 gaagacgggc gcgaccgccg tcgagagtct gcccgaccag aacctcggcc tcgccgtcat
     1681 cacgcagggg cgcggcggca aaatcatcac ggacgacgcg ctcaacctgc tcctccagcc
     1741 cgagcttcgc ggcggcgagg cacgctggta cgtctacggc gtgtgggatc aggagcagag
     1801 cgagctgctg aaggtgacgg cgaagagcgc cttcgagaag caccgcaacg gctcgatggt
     1861 gctgccctcg aaaggcgtca cgaccgaaga gggttttctg aaactcgtca gagacgcggg
     1921 cctccggatg tcgcagcccg tccgcgtcga ggtcgtgtcg aaggccgcgg cgccgcagtc
     1981 ggccccgccc ga
//
LOCUS       DBNGOH010000128         3002 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_201029, whole genome shotgun sequence.
ACCESSION   DBNGOH010000128 DBNGOH010000000
VERSION     DBNGOH010000128.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3002)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3002)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3002
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_201029"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            79..1065
                     /locus_tag="AC159P_02400"
     CDS             79..1065
                     /locus_tag="AC159P_02400"
                     /inference="COORDINATES: protein motif:HMM:NF015431.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR3694334.1"
                     /translation="MEIRQLRAFTAIAETRTFTAAAQRVHVTQAAISMQIRQLEQEVG
                     IPLFIRTPRRVVLTEAGEKLLERAYLILREHDAAVAEIAELAGAEHGRLRVGSSSATV
                     SAERLPHLFSEMLKVHPHVEITVTSGTSELLVKHILAGEIDVAFVSLPVEARNVETEV
                     LSHDQLVAIASPRHPLAKQKVVSAFALSGEKLILGERGGNTRRLIDEFFAEAGLKPTV
                     AMELSRQAAIKNMVAADMGVGIVPLSTAREDVERGRLVRWWIEGARINWELGLARLSG
                     GYTSPVCQTFLKLCRDYFAAEASVESAKSTREKKATSKAGAMQGGAKKRRGK"
     gene            complement(1067..1672)
                     /locus_tag="AC159P_02405"
     CDS             complement(1067..1672)
                     /locus_tag="AC159P_02405"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016873842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HIR3694335.1"
                     /translation="MLSEKFAEALAFAAGLHARQLRKGTDIPYVSHLLAVASIALEHG
                     ADEETTIAALLHDAIEDQGGDPTRQLIRRRFGERVADIVSRCTDAETVPKPPWRERKE
                     KYIEHVRREDDRAALLVSAADKLHNARSILNDYREDGEKVWERFKGGREGTLWYYDAL
                     IEAFRERGTSPLVEELARTVAELNRLASLPAETDAGRTTNL"
     gene            complement(1672..2217)
                     /locus_tag="AC159P_02410"
     CDS             complement(1672..2217)
                     /locus_tag="AC159P_02410"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012647359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KdsC family phosphatase"
                     /protein_id="HIR3694336.1"
                     /translation="MVEPEEIERRARRVRLLLMDCDGVLTDGRITLVGDAEEQKSFHT
                     RDGHGIVMLQRAGLRTGIISGRTSEAVVRRAADLRIEFVRQGSLDKVVQFDEVLAAAG
                     VSADETAYVGDDVVDIPLMRRSGFAVAVADATPETRAAAHYITHLAGGCGAVREVCEL
                     ILKAQGRWEELMKRYVDEAPT"
     gene            complement(2358..>3002)
                     /locus_tag="AC159P_02415"
     CDS             complement(2358..>3002)
                     /locus_tag="AC159P_02415"
                     /inference="COORDINATES: protein motif:HMM:NF025910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3694337.1"
                     /translation="ARAESSARAALARAPLSEDARIVLARAALEQNRLDEAEKEFRAA
                     LDSPLPSPATLAWGNIGLGEIALRRNRASDAAKRFDEAVRADAEYASTVAARAARIKA
                     ETAAAAPPIDEQLKAAVAQLDAAIRTGRKAEIDAAIVPGELSAFSRGLIGTQPEVWQT
                     RVLRTQSFGPHLAEADVALTTRALGRDASGTGVFIFVRTPAGWKLLDRRFFELQ"
BASE COUNT          478 a         1001 c         1025 g          498 t
ORIGIN      
        1 acccgtcgcg tataatgaaa actaaaagat tttatttccg cgataaccat acttgatacc
       61 ggagagcagc gacccccgat ggaaatacgc cagctacgcg cgttcaccgc aatcgccgag
      121 acccgcacct tcaccgccgc cgcccagcgc gtccacgtca cgcaggcggc catcagcatg
      181 cagatccgcc agctcgaaca ggaggtcggc atcccgctct tcatccgcac gccgcgccgc
      241 gtcgtcctga ccgaggcggg cgagaagctg ttggagcgcg cctacctcat cctgcgcgag
      301 cacgacgcgg cggtcgccga gatcgccgag ctggcgggcg ccgagcacgg gcgtttgcgc
      361 gtcggcagct cgtcggcgac cgtcagcgca gagcggctgc cgcacctctt ctccgagatg
      421 ctgaaggtgc acccgcacgt cgagatcacg gtcacttcgg gcaccagcga gctgctggtc
      481 aagcacatcc tcgcgggcga aatcgacgtg gccttcgtct ctctgccggt cgaggcgcgc
      541 aacgtcgaga ccgaggttct gagtcacgac cagctcgtcg ccatcgcttc gccccgccat
      601 ccgctcgcca aacaaaaagt ggtcagcgcc ttcgcgctct ccggcgagaa gctgatcctc
      661 ggcgagcgcg gcggcaacac gcggcggctc atcgacgagt tcttcgccga ggcgggactc
      721 aaaccgaccg tggcgatgga gctgagccgg caggccgcca tcaagaacat ggtcgcggcg
      781 gacatgggcg tcggcatcgt cccgctctcg acggcgcgcg aggacgtgga gcgcgggcgg
      841 ctcgtccgct ggtggatcga gggcgcgcgc atcaactggg aactcggcct cgcacgcctc
      901 agcggcggct acacctcgcc cgtctgccag acgttcctca agctctgccg cgactatttc
      961 gccgccgaag cgagcgtcga gtcggccaag agcacgcgcg agaagaaggc gacgagcaag
     1021 gcgggcgcga tgcagggggg cgccaagaag aggcggggga agtagtttag aggtttgtcg
     1081 tgcgacccgc gtccgtctcc gcgggcaagg atgcgagcct gttcaactcc gccaccgtcc
     1141 gcgcgagttc ttcgacgagc ggcgacgtgc cgcgctcgcg gaaggcttcg atgagagcgt
     1201 cgtaatacca gagcgtccct tcccggccac ccttgaaccg ctcccatact ttctcgccgt
     1261 cctcgcggta atcattcaga atcgagcgcg cgttgtgcag cttgtccgcg gcggaaacga
     1321 gcagcgccgc ccggtcgtcc tcacgcctga cgtgctcgat atatttttcc ttccgctcgc
     1381 gccacggcgg cttcggcacc gtctccgcgt ccgtgcagcg gctcacgatg tcggcgacgc
     1441 gctcgccgaa gcgccgccgg atcaactggc gcgtcgggtc gcctccctga tcttcaatcg
     1501 cgtcgtgcag gagggcggcg atggtggtct cttcgtcggc cccatgttcg agcgcgatac
     1561 tggcgaccgc cagcagatgc gagacgtaag ggatgtcggt tcccttccga agctgtcgcg
     1621 cgtgcaggcc cgcggcgaag gcgagcgcct ccgcgaattt ttccgagagg atcatgtcgg
     1681 tgcttcatcc acgtatcgct tcatcaactc ttcccagcgg ccctgcgctt tcagaatcaa
     1741 ctcgcagact tcgcggacgg cgccgcagcc gccggcgagg tgcgtgatgt agtgtgcggc
     1801 ggcgcgcgtc tcgggggtgg cgtcggcgac ggcgacggcg aagccggagc ggcgcatgag
     1861 cgggatgtca accacgtcgt cgccgacgta ggccgtctcg tccgccgaga cgcccgccgc
     1921 ggcgagcact tcgtcgaact gcacgacctt gtcgagcgaa ccctgccgga cgaactcgat
     1981 gcggaggtct gcggcgcggc gcacgaccgc ctcagaggtg cgccccgaga tgatgccggt
     2041 gcgcaggccc gcgcgttgca gcatgacgat gccgtggccg tcgcgcgtgt ggaagctctt
     2101 ctgctcttcc gcgtcgccga cgagcgtgat gcgcccgtcc gtcagcacgc cgtcgcagtc
     2161 catcaagagc agcctcacgc gccgcgctcg ccgctcgatc tcttccggct cgaccataat
     2221 cgaaatgatg aatgcggatt gatgaatgat gaatgagaag cggattgaat gatgaaaggc
     2281 agcgtgcctt tcatcattca tgaaaggcag cgtgtcttcc attcatcgtt catcattccg
     2341 cattcatcgt tagttcttca ttgaagctcg aagaagcgcc ggtctaaaag tttccagccg
     2401 gcgggcgtgc ggacgaagat gaagacgccg gtgccggagg cgtcgcgccc gagcgcgcgg
     2461 gtcgtgagtg cgacgtccgc ctcggcgagg tgcgggccga agctctgtgt gcggagcacg
     2521 cgggtctgcc agacttcggg ctgcgtgccg atgagtccgc gggagaaggc gctcagctcg
     2581 ccgggcacga tggccgcgtc gatctcggcc ttgcggcccg tgcggatggc ggcgtcgagc
     2641 tgcgcgacgg cggccttgag ctgctcgtcg atgggcgggg cggcagccgc cgtctcggcc
     2701 ttgatgcggg cggcgcgcgc ggcgaccgtc gaggcgtact cggcgtcggc gcgcacggcc
     2761 tcgtcgaagc gcttggcggc gtcgctcgcg cggttgcggc ggagcgcgat ctcgccgagt
     2821 ccgatgttgc cccaggcgag cgtggccgga ctcggcaggg gcgaatcgag cgcggcgcgg
     2881 aactccttct cggcctcgtc gaggcggttc tgttcgagcg cggcgcgcgc gagcacgatg
     2941 cgcgcgtctt cggagagcgg cgcgcgggct agcgccgcgc gcgcgctcga ctcggcgcgg
     3001 gc
//
LOCUS       DBNGOH010000129         6420 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4600344, whole genome shotgun sequence.
ACCESSION   DBNGOH010000129 DBNGOH010000000
VERSION     DBNGOH010000129.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6420)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6420)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6420
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4600344"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(164..2668)
                     /locus_tag="AC159P_02420"
     CDS             complement(164..2668)
                     /locus_tag="AC159P_02420"
                     /inference="COORDINATES: protein motif:HMM:NF024878.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HIR3694338.1"
                     /translation="MDDRKRSWLLLTFRVFLVACGAGVLGISLWRLAGTPFDARLVVL
                     ALLAVVASWIPTSQIPGDRGVVTASDTIIFLAVLLCGPDAAVLVAAAATASESARFTR
                     RWLNFGANVGLICCSLFVACGVVSLNFGDVRLLAHHSETFFLYALSLGMLAGMQAVIN
                     HGLVVPLISLKSAKPMLRMWRESYSWVFITYFTGVLTAAVVNAMIHYYGFGAIGFTIP
                     VLLANYLAYRPYLKNIEAARAHVEETRALHMRTLEAFATAVDAKDQITHEHVQRVQIY
                     AEGMARLLGLSDKEREALHAGALLHDIGKIAVPDYILNKPGKLTAAEFDRMKLHTVVG
                     AQILERIKFPYPLVPIVRHHHERWDGTGYPDGLKGEQIPLTARVLTVVDCFDAVREDR
                     QYRRAMTRQEAIDLLQKDSGTFYDPQVVKLFIEHLPTFEARIAEMKKGQKAFTPLAVE
                     QTEAIRKAAPAAGLAEERAEVAVGAGQPAEYLHTILAAHKSSHEIVALYELAQSFNGS
                     LAVSETLSVVTAKLEKLVPFDTCAVYLCDDAGGAARVEHAAGSDSESFRGRGVRQGEG
                     VTGWVIANNKQFANTDPALDLSALGNNLEGYRTLAVHPLVREGRVLGALALYSRALER
                     YTDDHLRTLGQVAGLTSDALHGAMLLAETRESGFTDQATGLPNARYLRAFFEQESARA
                     SRDEHRLAILAMDLNDFRRTSEALGDGGGGLLLQEVANEIRAQLRREDVLVREGGDRF
                     VALLRDTSPEVVGEIAVRVQSCMAGHYSAVPGAERVALDVTVGHARLDEDGDALDELL
                     EAAERRLQADKAAHRSFSQFARSGGAALTHRYNFIE"
     gene            complement(3128..4447)
                     /locus_tag="AC159P_02425"
     CDS             complement(3128..4447)
                     /locus_tag="AC159P_02425"
                     /inference="COORDINATES: protein motif:HMM:NF016232.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding domain-containing protein"
                     /protein_id="HIR3694339.1"
                     /translation="MARRKFRNQRHEPLGERSFQEYLTTAAPQTSRAELMTLVRGGED
                     TFLELKVKLSNPEKIAQGIVALANTGGGLIVFGVNDNLRVEGVDDAEAVRDELVRICR
                     DDISPALFPFIDIVAFDSGLRVVALDVTGKRRPYRTRDGRYFLRVGAEKREASREELS
                     ALMDEARPVRFENIAAVGASIEDVDEAHLWSFVREFEGDAFERERAERAYPTAEVLER
                     DLALAVPSAYGPVVGVAPTVAGLLLFGRDERVAELLPRSAITATRFAGDAAQAPKIET
                     VQLAGNLHTLYESALRFVERYCDLWDKRPRTFPGKETAEEAPVAARANYQRAAVAEAL
                     ANALAHRDLTLAGAATRLNVYDHSVEVTNPRRTSGLAPAMQRAIRYGVPQRLNPQLAA
                     LFASPAYGLTLPRGGLPMLLREARLFAGRKPDLQVFNDEFRVRLYGT"
     gene            complement(4564..6240)
                     /locus_tag="AC159P_02430"
     CDS             complement(4564..6240)
                     /locus_tag="AC159P_02430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694340.1"
                     /translation="MSQLVALIWLKWRLVRNSMRSRKAAAGRVASALTLALGLAISVG
                     VGAGLGVASYLSLSDVGRGVAESGEFFLLFIFVMIFMLWALVPLGMGGGSRFDAGRLL
                     LYPVSLKKLFAVDFVSELASLSTIFAAPSVLGLAVGAGLAKGNLAGALVAAALAFACG
                     LTLAKMLSTAVGSLMRRRRTRGETVLALLGGAVGLAGALMGQLAPYFARHAESLRGVR
                     WTPPGAAAVALSEGLSAGGGRAYVAALLTLAAYTTAFVVITYRIARRTALGIGGGSGR
                     ARERKTGAAAGGGEYAGWQLPLMSAEMSALVEKELRYALRNAQLKVVAMMAVGLTIVL
                     RLAPLGGARGRGGGGWERFSPYAEGAGTVFSVLYVFMLVSPLSTNLFGYDGAGLRALV
                     LAPVERWKILVGKNVALTLVSALLGVAAVVAGGLVFRDLSASTLAFAALAFASYAALF
                     ALAGNWLSLHFPKRVEFGKRMNRSGVAGLLLVPVFIALAAVPLSAVAAAHFAQSEALK
                     YVILGTFAAASFGLYLLLIGTQGRSLARRELDILEAVTGKSGEEGGQILG"
BASE COUNT         1061 a         2226 c         2229 g          904 t
ORIGIN      
        1 agaaggagtt cgtcgtcaag gcccgccagt tctacaactc gccgaaggac gcgaaggaca
       61 acaacaaaaa gtaagcgccc ccttcatccg ggaacggcga aggccgcggt gaacggttca
      121 ccgcggcctt cgccgttctc cacgaaccgt cgccgcgccg cgcctactcg atgaagttat
      181 agcggtgcgt cagggcggcg ccgccgctgc gcgcgaactg gctgaaggag cggtgcgcgg
      241 ccttgtccgc ttgcaggcgg cgctcggcgg cttcgagcag ctcgtcgagc gcgtcgccgt
      301 cttcgtcgag gcgggcgtgg ccgaccgtca cgtcgagcgc gacgcgctcg gcgcccggca
      361 ccgccgagta gtggccggcc atgcaggact ggacgcggac ggcgatctcg ccgaccacct
      421 cgggcgacgt gtcgcgcagc agcgcgacga agcggtcgcc gccctcgcgc acgagcacgt
      481 cctcgcgccg gagctgcgcg cgaatctcgt tcgccacctc ttgcagcagc agcccgccgc
      541 cgccgtcgcc gagcgcctcg ctcgtgcggc ggaagtcgtt caagtccatc gccaggatcg
      601 cgagccggtg ctcgtcgcgc gaggcgcgcg cgctctcctg ctcgaagaag gcgcgcaggt
      661 agcgcgcgtt gggcaggccc gtcgcttgat ccgtgaagcc gctctcgcgc gtctccgcca
      721 gaagcatcgc gccgtgcagc gcgtcggacg tgaggcccgc cacctgcccg agcgtgcgca
      781 ggtggtcgtc cgtgtagcgt tcgagcgcgc gcgagtagag cgcgagcgcg ccgagcacgc
      841 gcccctcgcg cacgagcggg tggacggcga gcgtgcggta gccttcgagg ttgttcccca
      901 gcgccgagag gtcgagcgcc gggtcggtgt tggcgaactg cttgttgttg gcgatgaccc
      961 agccggtgac gccctcgccc tgccgcacgc cgcgcccgcg gaaggactcg ctgtccgacc
     1021 cggcggcgtg ctcgacgcgc gcggccccgc ccgcgtcgtc acagagatag acggcgcagg
     1081 tgtcgaacgg caccagcttt tcgagcttcg cggtgacgac cgagagtgtc tccgagacgg
     1141 cgaggctgcc gttgaagctc tgcgccagct catagagcgc gacgatctcg tggctcgact
     1201 tgtgcgcggc gaggatggtg tggaggtact cggcgggctg cccggcgccg accgcgactt
     1261 cggcgcgctc ctcggcgagg ccggcggcgg gcgcggcctt gcggatggcc tcggtctgtt
     1321 cgacggcgag cggcgtgaaa gccttctgcc ccttcttcat ctcggcgatc cgggcctcga
     1381 aggtcggcag gtgctcgatg aagagcttga ccacctgcgg gtcgtagaag gtgccgctgt
     1441 ccttctgcaa gaggtcgatg gcctcctggc gcgtcatcgc gcggcgatac tgccggtctt
     1501 cgcgcacggc gtcgaagcaa tcgacgaccg tgagcacgcg cgccgtgagc ggtatctgct
     1561 cgcccttcag cccgtccggg tagccggtgc cgtcccagcg ctcgtggtgg tggcggacga
     1621 tgggcacgag cgggtacggg aacttgatgc gttcgaggat ttgcgcgccg accaccgtgt
     1681 gcagcttcat ccggtcgaac tcggcggcgg tgagcttgcc cggcttgttc aggatgtagt
     1741 cgggcaccgc gatcttgccg atgtcgtgca gcagcgcgcc ggcgtgcagc gcctcgcgct
     1801 ccttgtccga gaggccgagc aggcgggcca tgccctcggc gtaaatctgg acgcgctgca
     1861 cgtgctcgtg cgtgatctgg tctttggcgt ccacggcggt ggcgaaggct tcgagcgtgc
     1921 gcatgtggag ggcgcgcgtc tcttcgacgt gcgcgcgcgc ggcctcgatg ttcttgaggt
     1981 aggggcggta ggcgaggtag ttcgccagca gcaccgggat ggtgaagccg atggcgccga
     2041 agccgtagta gtgaatcatc gcgttgacga ccgccgccgt cagcacgccc gtgaagtagg
     2101 tgatgaagac ccacgagtag ctctcgcgcc acatgcggag catcggcttc gccgacttga
     2161 gcgagatgag cgggacgacc agcccgtggt tgatgacggc ctgcatgccg gcgagcatgc
     2221 cgagcgagag cgcgtagagg aagaaggtct cgctgtggtg cgcgagcagc cgcacgtcgc
     2281 cgaagttgag cgagacgacc ccgcacgcga cgaagagcga gcagcagatc agcccgacgt
     2341 tggcgccgaa gttgagccag cgccgcgtga agcgcgccga ctcgctggcg gtcgcggcgg
     2401 cggccaccag cacggcggcg tcggggccgc agagcagcac ggcgaggaag atgatcgtgt
     2461 ccgaggccgt gacgacgccg cggtcgcccg gtatctgcga ggtcggaatc cagctcgcga
     2521 ccacggcgag cagcgccagc acgacgagcc gcgcgtcgaa cggcgtgccc gccagccgcc
     2581 acagcgagat gcccagcacg cccgcgccgc acgcgacgag aaagaccctg aaggtcagca
     2641 ggagccagct gcgtttccta tcgtccatct ctgaaacccc acacacggcg acgggcgttg
     2701 agtctctcgt cgctgatttt gattgcggct tggattcgtg accgggcgga gcagaaaccg
     2761 gagggcgaga gcgtgtgcgg gcgcccgccg cacgtgagag cgcccgcgtc tttaaatagc
     2821 gaaacgcatg ccacgtcgcc ggtcgccgcc ggggtgcggg aatccgggca aaaacagggg
     2881 gttcggcgca gccgcccgtg accgccgacg ccactttgac cgggccgcct ccgcccctga
     2941 ccgattctga ccgaccaatg tgaccgggaa gagggggcga aaagggccaa atgggaaaag
     3001 ggggaaaggg gaaaagggga agaggatgat gaggagcagc gtggctctca tcaagtcgat
     3061 gatgagaggt agcgtgcctc ccgtcttccc tcttcgcctt ttcccctttt aaccttttcg
     3121 ccccttctta tgtgccgtag aggcggacgc ggaactcgtc gttgaagacc tgtaggtcgg
     3181 gcttgcgacc ggcgaagagt cgcgcctcgc ggagcagcat ggggaggcca ccgcgcggga
     3241 gcgtgaggcc gtaggcgggg ctggcgaaga gtgcggcgag ctgcgggttc aaacgctgcg
     3301 ggacgccgta gcggatggcg cgctgcatgg cgggcgcgag gccggaggtg cgccgcgggt
     3361 tcgtcacctc gaccgagtgg tcgtagacgt tgaggcgcgt cgccgcgccc gcgagcgtga
     3421 ggtcgcggtg cgcgagcgcg ttggcgagcg cttcggcgac cgccgcgcgc tggtagttgg
     3481 cgcgcgcggc gacgggcgcc tcctcagccg tctccttgcc ggggaacgtg cgcgggcgct
     3541 tgtcccagag gtcgcagtag cgctcgacga agcgcagcgc cgactcgtag agcgtgtgca
     3601 ggttgccggc gagttggacg gtctcgatct tcggcgcctg cgccgcgtcg cccgcgaagc
     3661 gcgtggcggt gatggcagag cgcggcagca gctccgccac gcgctcgtcg cgcccgaaga
     3721 gcagcaggcc ggcgaccgtg ggggcgaccc cgaccacggg gccgtaggcg ctcgggacgg
     3781 cgagcgcgag gtcgcgctca aggacttcgg cggtcgggta ggcgcgctcg gcgcgctcgc
     3841 gctcgaaggc gtcgccctcg aactcgcgga cgaagctcca caggtgcgcc tcgtccacgt
     3901 cctcgattga cgcgccgacc gccgcgatgt tctcgaagcg cacgggccgc gcctcgtcca
     3961 tcagcgccga gagttcctcg cgcgacgcct cgcgcttctc ggcgccgacg cgcaggaagt
     4021 agcgaccgtc gcgcgtgcgg taggggcggc gcttgcccgt cacgtcgagc gcgacgacgc
     4081 gcaggcccga gtcgaacgcg acgatgtcga tgaaggggaa gagcgcgggc gagatgtcgt
     4141 cgcggcagat gcggacgagt tcgtcgcgca ccgcctcggc gtcgtccacg ccctcgaccc
     4201 gcaggttgtc gttgacgccg aagacgatca gcccgccgcc cgtgttggcg agcgcgacga
     4261 tcccctgcgc gatcttctct gggttcgaga gctttacttt gagttcgagg aaggtgtctt
     4321 cgccgccgcg cacgagcgtc atcagctcgg cgcgcgaggt ctgcggggcg gcggtcgtca
     4381 ggtactcttg gaaagagcgt tcgccgagcg gttcgtgacg ctgattgcga aattttctgc
     4441 gagccatttg tgtcgagacg gggctgcaaa acccgcccgg atgctcccct cgcccgacgc
     4501 taagaagagc gaagaccgaa gcaccgctgg gatatcaaga gcggcgactt gtcacgtcgc
     4561 cgctcaacct agaatctggc cgccctcttc gccggacttg ccggtcacgg cctccaggat
     4621 gtcaagctcg cgccgcgcga gcgagcgccc ttgcgtcccg atcagtaaca ggtaaagccc
     4681 gaaggaagcg gcggcgaacg tgccgagtat aacatacttg agggcttcgc tttgggcgaa
     4741 gtgggcggcg gcgaccgccg agagcggcac cgcggccagc gcgatgaaga ccggcacgag
     4801 cagcagcccc gcgacgcccg agcggttcat ccgcttgccg aactcgacgc gcttcgggaa
     4861 gtggagcgag agccagttgc cggcgagcgc gaagagcgcc gcgtaggacg cgaaggcgag
     4921 cgccgcgaac gccagcgtcg atgctgacag gtcgcggaag accagcccgc ccgcgaccac
     4981 cgccgccacg ccgagcagcg ccgagaccag cgtcagcgcg acgttcttcc cgacgagtat
     5041 cttccagcgc tcgaccggcg cgagcaccag cgcgcgcaag cccgcgccgt cgtagccgaa
     5101 gaggttcgtc gagagcggcg agacgagcat gaacacgtag agcacgctga agaccgtgcc
     5161 cgccccttcg gcgtaagggc tgaaccgctc ccacccgccg ccgccgcgcc cgcgcgcgcc
     5221 gccgagtggc gccagccgca gcacgatcgt cagccccacc gccatcatcg ccaccacctt
     5281 caactgcgcg ttgcgcaacg cgtaccgcag ctccttctcc acgagcgccg acatctcagc
     5341 cgacatcaaa ggcaactgcc agcccgcgta ctcgccgccc ccagccgccg cgcccgtctt
     5401 ccgttcgcgc gcgcggcccg agccgccgcc tatccccagc gccgtccgcc gcgcgatgcg
     5461 gtacgtgatg acgacgaagg ccgtcgtgta cgcggcgagc gtcagcagcg cggcgacgta
     5521 agcgcgcccg ccgcccgcac tcaatccctc ggacagcgcg acggcggcgg cgcccggcgg
     5581 cgtccaacgc acgccgcgca gagactccgc gtgccgcgcg aagtagggcg cgagctgacc
     5641 catcaacgcg cccgccagcc cgaccgcccc gccgagcagc gccagcaccg tctcgccgcg
     5701 cgtccgcctc cgcctcatca aagagccgac ggcggtcgag agcatcttcg ccagcgtcag
     5761 cccgcacgcg aacgcgagcg ccgccgccac gagggccccc gccaagttcc cttttgccag
     5821 ccccgccccc accgcgaggc cgagcaccga cggcgccgcg aagatcgtcg agaggctcgc
     5881 cagctcgctc acgaaatcca ccgcgaacag cttcttcagc gacacgggat agagcaacag
     5941 tcgccccgcg tcgaaccggc tgcccccgcc catccccagc ggcaccagcg cccacagcat
     6001 gaagatcatc acgaagatga acagcagaaa gaactcgccc gactccgcga ccccacgccc
     6061 cacgtccgac aacgacaggt acgaagccac ccccagcccc gcccccacgc ccacggagat
     6121 cgccagcccc aacgccagcg tcaaagccga agccacccgc cccgccgccg ctttgcgcga
     6181 ccgcatcgag ttccgcacca gcctccactt caaccagatg agcgcgacga gttgcgacat
     6241 gggttacaca gtcgagaaca ccccgagcgt tatcgcgcgc cccgactcca tgacgacacc
     6301 gcgcgcccgg ttgacgtatt atgctacgca cgatagcacg ggcagaagcc ggcgcctttc
     6361 gacgacgccg gattcatcca cttcgggatt caaacgatgg taatcatccg cgccgaaacg
//
LOCUS       DBNGOH010000130         3214 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2401047, whole genome shotgun sequence.
ACCESSION   DBNGOH010000130 DBNGOH010000000
VERSION     DBNGOH010000130.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3214)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3214)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3214
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2401047"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            94..>3214
                     /locus_tag="AC159P_02435"
     CDS             94..>3214
                     /locus_tag="AC159P_02435"
                     /inference="COORDINATES: protein motif:HMM:TIGR00757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HIR3694341.1"
                     /translation="MPKELIVSVIGREKKIAIVEDGRTTEFYIERGEGSQGIVGNIYK
                     GRVMRVLPGMQSAFVDIGLERDAFLYVSDFFDEEEEFERIVVDKTKTEGADADSAARA
                     AAERIERTRIERERQIETAHESAEPEGGKETDGEEEEAPAVAAAADTSSAVEPPVALT
                     GEQEAAATASADEGKEAGGRRGRERCRRRGGDREATPQEGAARAPEPTVVYEGASHDV
                     STPFAAGADDFHRIVDEEDSAAEDGAMFKDARVQERLLDQIHAVEFDMETTGAAEVGS
                     LLAAGATGEGAFERIADDEEEQTASGRARRAVQPEIAASVARLADALSDEDTFTSSAQ
                     TFERVSDDESAEAVAAATGKGASKRGAGRGASKRSGSKKAATKGAAEAEPESAAAAPG
                     EGAEEGATKKRASRSREASAGKASKKAATKGASKKSAGKGRGRGAKAGEVADEGAPEG
                     GADNASLGDAEAKVTARTDRGEFARRGGRRRRRPGGKPGEDGNGNEQGDGQNGDAEVI
                     VEAEAPRVEEPVAVAAAASVSNAPSPAPPPSQSRSRGRRDGRDERGERGGRGERSDRG
                     GERGGERGERSGERSSSGEQRSSERGERGERSERGERSGPPAREGREGRDGRDGGREG
                     RRRERQPTITDLLREGQEIIVQIAKEPIAQKGARITSHIALPGRFLVYMPTIEHVGVS
                     RKIESDSERVRLRKLIQNIVKTEDLPSGGFIVRTAGVGISEQDLRDDARYLIRTWRDI
                     RRNAERVKAPAIAHKDLDLVQRILRDQLSDDFTAIRIDNEEEYLDVVEFVNRIQPKLV
                     NRVKLYTRDEPILEHYGVQAEIDKAIKPRVWLKSGGYLVINQTEALVAIDVNTGKFVG
                     RGGSRLEDTITRTNMEAVEEIARQIRLRDLGGILVLDLIDMEERRNRARVLQALQDAL
                     RQDKSPTKVLSFNDFGLVIMTRKRVKQSLERTLCSPCPYCTGAGLVKSPQTICYEILE
                     EARSLSRSSEGEHHKQTTLRVNPEVARGLHPSCAPARSTGARDAPIPPVCACDSSPSR
                     SSPSSCRAAS"
BASE COUNT          593 a         1089 c         1170 g          362 t
ORIGIN      
        1 gatttccaaa accgcggcca gggacgcgcc ctgcctctct cacgagtgcg ggaggcgctc
       61 gccgccacta gacaggagac agtttcaacc atcatgccga aagagctaat cgtcagcgtc
      121 atcgggcgcg aaaagaagat cgcgatcgtc gaggacggtc gcaccacgga gttctacatc
      181 gagcgcggcg aggggagcca gggcatcgtc ggcaacattt acaaggggcg cgtgatgcgc
      241 gtcctgcccg gcatgcagtc ggccttcgtg gacatcgggc tggagcgcga cgcgttcctc
      301 tacgtctcgg acttcttcga cgaggaggag gagttcgagc gcatcgtcgt cgataagacg
      361 aagaccgagg gcgccgacgc cgactccgcg gcgcgcgccg ccgccgaacg catcgagcgc
      421 acgcgcatcg agcgcgagcg gcagatcgag acggcgcacg agagcgccga acccgaaggc
      481 gggaaagaga ccgatggcga agaagaagag gcgcctgcgg tcgccgccgc cgctgatact
      541 tcgtcggcgg tcgagccgcc cgtcgcgttg acaggcgagc aggaggccgc ggcgaccgcg
      601 tcggcggacg agggcaagga ggcgggcggt cggcgcggtc gcgaacgttg tcgccgccgc
      661 ggcggcgaca gagaggcgac gccgcaggaa ggggcggcgc gggcgcccga gccgaccgtc
      721 gtctacgagg gcgcgagcca cgacgtgagc acgccgttcg cggcgggcgc ggacgacttc
      781 caccgcatcg ttgacgaaga agactccgcc gccgaagacg gcgcgatgtt caaggacgcg
      841 cgcgtgcagg agcgcctgct cgaccagatt cacgccgtcg agttcgacat ggagacgacc
      901 ggcgcggcgg aggtcggctc gctgctcgcg gcgggcgcga ccggcgaagg cgcgttcgag
      961 cgcatcgccg acgacgaaga agagcagacg gcgagtggcc gcgcccgccg cgccgtgcag
     1021 cccgagatcg ccgcctccgt cgcgcggctc gcggacgcgc tgagcgacga agacactttc
     1081 acttcgagcg cgcagacgtt cgagcgcgtc agcgacgacg agtccgccga ggccgtggcc
     1141 gccgcgaccg ggaagggcgc gagcaagcgc ggcgcgggcc gcggcgcctc gaagcgctca
     1201 ggctcgaaga aggccgcgac gaagggcgcg gcggaagcag agccggagag cgccgccgcc
     1261 gcgccgggag agggcgcgga ggaaggcgcg acgaagaagc gcgcttcgcg ttcgcgcgag
     1321 gcttcggcgg gcaaggcttc gaagaaggcc gcgacgaagg gcgcgagcaa gaagtccgcc
     1381 ggcaaggggc gcgggcgcgg cgcgaaggcg ggcgaggtag cggacgaagg cgcgcccgag
     1441 ggcggcgccg acaacgcctc gctcggagac gccgaggcga aggtcacggc gcgcacggat
     1501 cgcggcgagt tcgcgcggcg cggcggtcgt cgtcgccgca ggcccggcgg caaaccgggc
     1561 gaggacggca acggcaacga gcagggcgac ggacagaacg gcgacgcgga ggtcatcgtc
     1621 gaagcggaag cgccgcgcgt cgaagagccg gtcgcggtcg ctgcggcggc gtcggtaagc
     1681 aacgcgccgt cgcccgcgcc tccgccgtcg cagagcagaa gccgcgggcg ccgtgatggc
     1741 cgcgacgagc gcggcgagcg cggcggtcgc ggcgaacgca gtgaccgcgg cggcgagcgc
     1801 ggcggtgaac gtggtgaacg cagtggtgaa cgcagcagca gtggcgagca gcgtagcagt
     1861 gaacgcggcg agcgcggcga acgcagcgaa cggggcgaac gtagtggccc gcccgcgcgc
     1921 gagggccgcg aaggtcgcga cggtcgtgac ggcggtcgcg aaggccgtcg gcgcgagcgc
     1981 cagccgacga tcacggactt gctccgcgaa ggtcaggaga tcatcgtcca gatcgcgaag
     2041 gaacccatcg cgcaaaaagg ggcgcgcatc acctcgcaca tcgcgctgcc cggtcgcttc
     2101 ctcgtctaca tgccgaccat cgagcacgtc ggcgtctcgc gcaagatcga gtcggactcc
     2161 gagcgcgtgc gcctccggaa gctcatccag aacatcgtca agaccgaaga cctgccgtcg
     2221 ggcggcttca tcgtccgcac ggcgggcgtc ggcatctcgg agcaggacct gcgcgacgac
     2281 gcgcgctacc tcatccgcac gtggcgcgac atccgccgca acgccgagcg cgtgaaggcc
     2341 ccggccatcg cgcacaaaga tctcgacctc gtgcagcgca tcctgcgcga ccagttgtcg
     2401 gacgacttca ccgccatccg catcgacaac gaggaggagt atctggacgt ggtcgagttc
     2461 gtcaaccgca tccagccaaa gctcgtcaac cgcgtcaagc tctacacgcg cgacgagccg
     2521 atcctcgaac actacggcgt gcaggccgag atcgacaagg ccatcaagcc gcgcgtctgg
     2581 ctcaagtcgg gcggctacct cgtcatcaac cagaccgagg cgctggtcgc catcgacgtg
     2641 aacacgggca agttcgtcgg gcgcggcggg agccgcctcg aagacacgat cacgcgcacc
     2701 aacatggagg cggtcgaaga gatcgcgcgg cagatacggc tgcgcgatct cggcggcatc
     2761 ctcgtcctcg acctcatcga catggaggag cgccgcaacc gcgcgcgcgt gctgcaagcc
     2821 ttgcaggacg cgctccggca ggacaagtcg ccgacgaagg tgctctcgtt caacgacttc
     2881 ggcctcgtca tcatgacgcg caagcgcgtc aagcagtcgc tcgaacgcac gctctgctcg
     2941 ccctgcccct actgcacggg cgccgggctg gtcaaatcgc cgcagacgat ctgctacgag
     3001 atactcgaag aggcgcgcag cctctcccgc tccagcgagg gcgaacacca caagcagacc
     3061 acgctccgcg tcaaccccga agtcgcgcgc ggcctgcatc cgagctgcgc acccgcccgc
     3121 tcaaccggcg cccgggatgc gccgattcct cctgtatgcg cctgcgactc ctcgccctcg
     3181 cgctcgtcgc ccagctcgtg tcgggctgct tcgt
//
LOCUS       DBNGOH010000131         3035 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4233760, whole genome shotgun sequence.
ACCESSION   DBNGOH010000131 DBNGOH010000000
VERSION     DBNGOH010000131.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3035)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3035)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3035
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4233760"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(155..847)
                     /locus_tag="AC159P_02440"
     CDS             complement(155..847)
                     /locus_tag="AC159P_02440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694342.1"
                     /translation="MKVFLSAALLLFAFSSAAAAQDERRFLDAKLVRAEGKAAEDFVP
                     RGWVLEEQATGDLNGDALPDAALRLIEDLPPEAADGTWNMRHRALVLIFKRADGAYAR
                     AAVATRVLYCSLCGGALSDPSGGNLAVSIEKGVLNVFQLSGAREAHELTQRFRYDPRA
                     KRFALIGEDVETYDRADGDGTKTSTNYLTGLQVTEKRRLLKEGQEPKLVSTTRKKITA
                     PRRFIEDIDYEQ"
     gene            complement(910..1863)
                     /locus_tag="AC159P_02445"
     CDS             complement(910..1863)
                     /locus_tag="AC159P_02445"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012515.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HIR3694343.1"
                     /translation="MRFPFELPAAREFDAVGFGLNAVDHLIVVPAYPAFDTKVRLVEH
                     AQSAGGQTASTMVALKRLGLRTAYAGRFGSDAEGRFGFETLRAEGVNIECAEMIEGAR
                     NQIAYIVIDARTGERTVIWDRDERLAYAPDEVPHGFGARGRVLHMDAHDPPACARVAR
                     EAREGGAVVSLDVDNIYEGLPGLLPFVDVLVSSKEFPRRLTGIDDERAALVETKARLR
                     PEAVVGMTLGERGAVIYHDGAFVEAPAFAVPGGCRDTTGAGDAFHAGFLYGMLQGEEI
                     EACLTLGCAVAALKCRALGARSALPSAWELNQLLSGEGETS"
     gene            2050..>3035
                     /locus_tag="AC159P_02450"
     CDS             2050..>3035
                     /locus_tag="AC159P_02450"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HIR3694344.1"
                     /translation="MRHRLPRVCLSLLMCFVAAGATAPPAALAQVPPRCQPPALSASG
                     KERNIFTPEQESDLGDAIAEHVQRHYILIEEEEVTGRLRAIGAALVKQIPPTDLRFQF
                     FLVDLPDVNAFAMPGGRIYVSRKLVAFAESEDELAGVLAHEIGHLAARQTAIDMTRIF
                     KKVLGVTKVTDRRDIFEKYNQLVENTARKPGAFGNGEEEKEQLEADRIGLYAMVAAGY
                     DPQAFATFFDRLAETKGRTGGFLSDLFRKTSPDSRRLREILKGLQTLPAECRAARPAA
                     AAAQFEKWRAAVVGFSGAGRKEALHDVLSKKALEPPLRGDINHLRFSPDGKYL"
BASE COUNT          482 a         1066 c         1022 g          465 t
ORIGIN      
        1 caaacgcccc cgattcgtag aggcagtagg cagtaggcag caggcagtag gcagtcaaag
       61 ataattacga atcaccgatt cgaaatttcg aatctcgaat ctcaaatccg gtatcgcttt
      121 atctgcctac tgccttctgc ctgctgccta ctgcctactg ctcatagtcg atgtcttcga
      181 tgaagcggcg gggggcggtg atctttttgc gggtggtgga gacgagtttg ggttcctggc
      241 cttctttgag gaggcggcgc ttttcggtga cctggaggcc ggtaaggtag ttggtgctgg
      301 tcttggtgcc gtcgccgtcg gcgcggtcgt aggtctccac gtcttcgccg atgagggcga
      361 agcgtttggc gcgggggtcg tagcggaagc gctgcgtgag ttcgtgggcc tcgcgggcgc
      421 cgctgagctg gaagacgttg aggacgccct tctcgatgga gacggcgagg ttgccgcccg
      481 aggggtcgct cagcgccccg ccgcagagcg agcagtagag cacgcgcgtc gccacggcgg
      541 cgcgcgcgta ggcgccgtcg gcgcgtttga aaatcaggac gagcgcgcgg tggcgcatgt
      601 tccatgtgcc gtccgccgcc tcgggcggca ggtcttcgat gagcctgagc gcggcgtcgg
      661 gcagcgcgtc gccgttgagg tcgccggtgg cctgctcttc gagcacccag ccgcgcggga
      721 cgaaatcttc cgccgccttc ccttcggcgc ggacgagctt cgcgtcgagg aagcgccgct
      781 cgtcctgcgc cgccgcggcg gacgagaagg cgaagagcaa cagcgccgca ctcaggaaaa
      841 ctttcatcgc gacaacacct cccgcacgcc gcgacttcga agtcgcaacg aacgctgccg
      901 aacagtcttc taagaggtct cgccctcgcc cgacaggagt tggttcaact cccacgcgga
      961 ggggagcgcc gagcgcgcgc cgagcgcgcg gcacttgagc gcggcgacgg cgcagccgag
     1021 cgtgagacac gcctcgatct cctcgccctg caacatgccg tagaggaagc ctgcgtggaa
     1081 ggcgtcgccc gcgccggtcg tgtcgcggca gccgccgggc acggcgaagg cgggcgcttc
     1141 gacgaaggcg ccgtcgtggt agatgacggc gccgcgctcg ccgagcgtca tgccgacgac
     1201 tgcttcgggg cgcagccgtg ccttcgtctc gacgagcgcg gcgcgttcgt cgtcgatgcc
     1261 cgtcaggcgg cgcgggaact ccttcgaaga gacgagcacg tccacgaagg gcagcaatcc
     1321 gggcaacccc tcgtagatgt tgtccacgtc gagcgagacg acggcgccgc cctcccgcgc
     1381 ctcgcgcgcg acgcgggcgc aggccggcgg gtcgtgcgcg tccatgtgca ggacgcgccc
     1441 gcgcgcgccg aagccgtgcg gcacttcgtc cggcgcgtag gcgagccgct cgtccctgtc
     1501 ccagatgacc gtgcgctcgc ccgtgcgcgc gtcgatgacg atgtaggcga tctggttgcg
     1561 cgcgccctcg atcatctcgg cgcactcgat gttgacgcct tcggcccgca acgtctcgaa
     1621 gccgaagcgc ccctcggcgt ccgagccgaa gcgaccggcg taggccgtgc gcaggccgag
     1681 ccttttgagc gccaccatcg tcgaggcggt ctgcccgccc gccgactgcg cgtgttcgac
     1741 gaggcgcacc ttcgtgtcga aggcggggta ggcgggcacg acgatcaggt gatcgacggc
     1801 gttcagtccg aagcccacgg cgtcgaactc tctggcggcg ggcagctcga aagggaagcg
     1861 cacgggctac tgtttctctg acctcgattc cggttttgcg gcgcgaacgg ccatacggta
     1921 acatctccgc cgtcgagctg cccaaccgcg ggcgccctgc ccgcatcgaa agacgcaaac
     1981 cctccgaact ctcccgcggc gggtgacgac gcccggcgtg ccgccgcccg aacggcaaag
     2041 gaatgcgaca tgagacaccg cctgccccgc gtctgcctct ctctgctcat gtgcttcgtc
     2101 gcggcgggcg cgaccgcgcc gcccgccgcg ctcgcgcaag tcccgccgcg ctgccagccg
     2161 cccgcgctct cggcctccgg caaggagcgg aacatcttca cgcccgagca ggagagcgac
     2221 ctcggcgacg ccatcgccga gcacgtccag cgccactaca tcctcatcga agaggaggag
     2281 gtgacgggcc gcctgcgcgc catcggcgcc gccctcgtca agcagattcc gccgaccgac
     2341 ctccgcttcc agttcttcct cgtcgatctg ccggacgtga acgccttcgc catgccgggc
     2401 gggcgcatct acgtctcgcg caagctggtc gccttcgccg agagcgagga cgagctggcc
     2461 ggagtgctcg cccacgagat cggccacctc gccgcgcgcc agaccgccat cgacatgacg
     2521 cgcatcttca agaaggtgct cggcgtgacg aaggtcacgg accggcgcga catcttcgag
     2581 aagtacaacc agctcgtcga gaacacggcg cgcaagcccg gcgccttcgg caacggcgag
     2641 gaggagaagg agcagctcga agccgaccgg atcggcctct atgcgatggt cgccgccggc
     2701 tacgacccgc aggcgttcgc caccttcttc gaccgcctcg ccgagaccaa aggccggacg
     2761 ggcggcttcc tctccgacct cttccgcaag accagccccg actcgcgccg cctgcgcgaa
     2821 atcctcaagg ggctgcaaac gctccccgcc gagtgccgcg ccgcacggcc cgccgccgcc
     2881 gccgcgcagt tcgagaagtg gcgcgccgcg gtcgtcggct tctcgggcgc gggccgcaaa
     2941 gaggcgctgc acgacgtgct ctcgaagaag gcgctcgaac cccccttgcg cggcgacatc
     3001 aaccacctgc gcttcagccc cgacggcaag tacct
//
LOCUS       DBNGOH010000132         2578 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4233801, whole genome shotgun sequence.
ACCESSION   DBNGOH010000132 DBNGOH010000000
VERSION     DBNGOH010000132.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2578)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2578)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2578
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4233801"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..349)
                     /locus_tag="AC159P_02455"
     CDS             complement(<1..349)
                     /locus_tag="AC159P_02455"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF013308.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IspD/TarI family cytidylyltransferase"
                     /protein_id="HIR3694345.1"
                     /translation="MNVAIIAAGGAGTRAGGGRAKQFREISGTPIIIHTLRRFERCAR
                     IERVVVVAPAGALEEARILAAEHGLRKVARVVAGGGSRLESVWEGLRVAGEWRPAGVV
                     AVHDAVRPFVTPDE"
     gene            complement(432..1556)
                     /locus_tag="AC159P_02460"
     CDS             complement(432..1556)
                     /locus_tag="AC159P_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HIR3694346.1"
                     /translation="MGTNTALIRIIFVGILVAAGYVLRPVPGMFGLSSGIVSAFVAAL
                     VAASAILFEIRIQMASLKTLIGAAIGSILGIVGGVLIGFLISYQESAAVNHSTKVFLT
                     LMLVFLMAYVGLMVGAAKGDYLDLSALGGIFSDKANRRDMKILDTSVIIDGRIADVAE
                     TGFLSGTIIIPQFILRELQQVADSPDSSKRQRGRRGLDMLNRLQGNSTLDIQVVDTDF
                     PAVKEVDLKLLELSKQLDAVVVTNDFNLNKVAQLHGIQVLNINELANSLKPVVLPGEG
                     MRVFILKEGKEYNQGVAYLDDGTMVVVDNARRMIGKNADISVTSVLQTTAGKMIFGRL
                     WEDSENGAPGEARVANGGGSRRPYQQRERAPVAPSTAKEQ"
     gene            complement(1604..>2578)
                     /gene="radA"
                     /locus_tag="AC159P_02465"
     CDS             complement(1604..>2578)
                     /gene="radA"
                     /locus_tag="AC159P_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HIR3694347.1"
                     /translation="QIKLRGERLAVEAKNVFILPETNLENIINEIERLKPGAIIVDSI
                     QTVFSSKIESAPGSVSQVREVAGQFLLLAKNRTIPVFLIGHVTKEGSIAGPKALEHIV
                     DTVLYFEGERHHNHRIIRATKNRFGAANELGVFEMTGTGLVPVANPSEMFLKERPQDV
                     AGSVVTACMEGTRPVLVEVQSLVAGSKYGTGRRMAQGLDVNRVALLIAMLEKRAGFHL
                     MGDDVFVNVAGGMEIDEPAADLGVVAAITSSFKNRAIDPQTAVFGEVGLTGEVRGAMQ
                     AQARAREAQALGFKKIVMPASNTAGLERLLGVRVVGVRSVEEALDELF"
BASE COUNT          445 a          865 c          820 g          448 t
ORIGIN      
        1 tctcgtccgg cgtgacgaag gggcgcaccg cgtcgtggac ggcgacgaca cccgccgggc
       61 gccactcgcc cgcgacccgc agaccttccc acaccgattc gagccgcgac ccgccgcccg
      121 cgacgacgcg cgccaccttg cgcaggccgt gctccgcggc gaggatgcgc gcctcttcca
      181 gcgcgcccgc cggggccacg accacgaccc tctcgattcg ggcgcaccgc tcgaaccgtc
      241 tcagcgtatg aatgatgatg ggggtgccgg agatttcccg gaactgcttg gcccgcccgc
      301 cgcccgcccg cgtccccgcg ccgccggcgg cgatgatcgc gacattcata aaagccgtga
      361 accgtaaaca gtgaaccgtg aaccgtgaca gagaagaccg tttttactca ttacggttca
      421 cggttcacgg ttcactgttc tttggctgtg ctcggggcga ccggggcgcg ctcgcgctgc
      481 tgataggggc ggcgagagcc gccgccgttg gcgacgcggg cttcgccggg cgcgccgttc
      541 tcgctgtcct cccagagccg cccgaagatc atcttgccgg cggtggtctg caagacgctc
      601 gtcacggaga tgtcggcgtt cttgccgatc atgcgccgcg cgttatcgac gacgaccatc
      661 gtcccgtcgt cgaggtaggc gacgccctgg ttgtactcct tgccctcttt caggatgaag
      721 acgcgcatcc cctcgccggg cagcaccacg ggcttcagcg agttcgccag ctcgttgatg
      781 ttcaagacct gtatgccgtg gagctgcgcg accttgttga ggttgaagtc gttggtgacg
      841 acgacggcgt cgagctgctt cgagagttcg aggagcttga gatcgacctc cttgacggcg
      901 gggaagtccg tgtccacgac ctggatgtcg agcgtcgagt tcccttgcag gcggttgagc
      961 atgtcgaggc cgcggcggcc ccgctgccgc ttcgaggagt cgggcgagtc ggccacttgt
     1021 tgcagctcgc ggaggatgaa ctgcgggatg atgatggtgc ccgacaaaaa gccggtctcg
     1081 gccacgtcgg cgatcctgcc gtcgatgatg acggaggtgt cgagaatctt catgtcgcgc
     1141 cggttggcct tgtcgctgaa gatgccgcct aaagcagaga ggtctaagta gtcgcccttc
     1201 gccgcgccga ccatcagccc gacgtaggcc atcaggaaga cgagcatcag cgtgaggaag
     1261 accttcgtgc tgtggttgac cgccgccgac tcctgatagc tgatgaggaa gccgatcagc
     1321 acgccgccga cgatccccaa gatcgaaccg atggccgcgc cgatcagggt cttcagcgag
     1381 gccatctgga tgcgtatctc gaagaggatg gcggaggcgg cgacgagggc ggcgacgaag
     1441 gccgaaacga tgcccgagga gagaccgaac atgcccggca cggggcgcaa cacgtacccc
     1501 gccgcgacga ggattccgac gaagatgatg cgaatcagag ccgtgtttgt gcccatgacg
     1561 aacttaatat taacacggca ttaacccgcg cgctacgaaa aggttaaaac aactcgtcga
     1621 gcgcctcctc gaccgaacgg acgcccacca cgcgcacccc gagcagccgc tccagccccg
     1681 ccgtgttcga cgcgggcatc acgattttct tgaagccgag cgcctgcgcc tcgcgcgcgc
     1741 gcgcctgcgc ctgcatcgcg ccgcgcacct cgcccgtcag gcccacttcg ccgaagaccg
     1801 ccgtctgcgg gtcgatcgcg cggttcttga acgaagacgt gatggccgcg accacgccga
     1861 ggtctgccgc cggctcgtcg atctccatcc cgcccgcgac gttgacgaac acgtcgtcgc
     1921 ccatcaggtg aaagcccgcg cgcttttcga gcatcgcgat caagagcgcg acgcggttca
     1981 cgtccaagcc ctgcgccatc cgcctcccgg tgccgtactt cgagccggcg acgagcgact
     2041 ggacttcgac cagcacgggc cgcgtgccct ccatgcacgc cgtcacgacc gagccggcca
     2101 cgtcctgcgg gcgctccttc aaaaacatct ccgaggggtt ggcgaccggc acgagtcccg
     2161 tccccgtcat ctcgaagacg ccgagttcgt tcgccgcgcc gaagcggttc ttcgtcgcgc
     2221 ggatgatgcg gtggttgtga tggcgctcgc cctcgaagta gagcaccgtg tccacgatgt
     2281 gctccaaagc cttcggcccc gcgatggagc cttccttcgt cacgtggccg atgaggaaga
     2341 ccgggatcgt gcggttcttc gccagcaaca ggaactgccc cgccacctcg cgcacctgcg
     2401 agaccgagcc gggcgccgac tcgatcttcg aggagaagac cgtctggatc gaatcgacga
     2461 tgatcgcccc aggctttagc cgttcgatct cgttgatgat gttttcaagg ttggtctcag
     2521 gcaggatgaa gacgttcttt gcttcgacgg cgagccgttc gccgcggagc ttgatctg
//
LOCUS       DBNGOH010000133         3549 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2767688, whole genome shotgun sequence.
ACCESSION   DBNGOH010000133 DBNGOH010000000
VERSION     DBNGOH010000133.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3549)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3549)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3549
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2767688"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..785
                     /locus_tag="AC159P_02470"
     CDS             <1..785
                     /locus_tag="AC159P_02470"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3694348.1"
                     /translation="AEKEQIRHQLFQSSKLASVGELSAGVAHEINNPLNGIINFAQLL
                     KDDEVARTDGQRRMVDGIIDEGERIARIVRNLLTFARQDPHELTRVNIAETIDASLSL
                     FGHQLRKDGIAVEIAVEENLPPVMADGPRLRQVMVNMVSNAHHALRAKAGGEKVFRTT
                     ARAVETAGGPAVRIEFYDTGVGIRREDIDKVFDPFFTTRRATGGTGLGLSLSFGIVRE
                     YGGTITVESAEGDFTRFCVELPAAELREVKHDERAESAVGGR"
     gene            748..1914
                     /locus_tag="AC159P_02475"
     CDS             748..1914
                     /locus_tag="AC159P_02475"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3694349.1"
                     /translation="MTSAPRVLLVDDEPNMRWTMAEFLRRGGYEPLTASDFESATELL
                     RDAEVDAAVVDIVLPGKSGVELLKELRGREPYVPVVMITGEPNSVLVPEIVRAGAYDY
                     IAKPIVKDALIKAVARAVERKRLEDEKRRLEREIKHHAEELEARVAERTAQLVEAHER
                     LAHQEKIAALGRAAAQVAHEVKNPLAGLLLYAMHLKTKAAGKLAEGELALVDKIVETI
                     NHLTDTVEQVLNFARPVCVTPRPGDLNRVVSDVLQLAQPQMAGGLIEQRLELDERGCR
                     GMLDETALRGALMNLVLNAVQAMPGGGTLSVRTAADGAALRLEVADTGCGMTEEQIGH
                     VFEPFYTTKSQGLGLGMPYAQKVIEQHRGTVCIESRPGAGTTIRVELPAETGAG"
     gene            1946..3361
                     /locus_tag="AC159P_02480"
     CDS             1946..3361
                     /locus_tag="AC159P_02480"
                     /inference="COORDINATES: protein motif:HMM:NF025885.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HIR3694350.1"
                     /translation="MPRSSRILVVDDEASVTGALELVLGDRGHVVASARSVGAASELL
                     KGDPFDLVFTDLRLPDGSGIDLLTSIKRDAPDIEVVLMTAHGSLDVTIEAIKRGAFYY
                     LEKPFTPDQALVLAARALQFKAMKNENRVLRATRPSDGEDFGLLGRHPKLRQIHEVIR
                     TAAPSDASVLIEGESGTGKELIAAALHLKSERAARPFIRINCAAIPSELIESELFGYR
                     KGAFTGADRDKRGLVEASAGGTLLLDEIAEMPAHLQTKLLRVLQERRLRRLGDEHEVV
                     VDFRLISSTNRDTAQMIQEGALRKDLYFRISTIKVKVPPLRERLDDVMLLAEHFLQRY
                     AEKYRKRVRGLSQAAYSLLARYDWPGNVRELEGVVEHAVLFAQDEQLMPEDLPEHLHA
                     AQATHFRCVIPPYLTMEEIEREAIAQTLERTGGNVKKTAQILDYHRPTLYRKLRRFGL
                     RDAGDDKNAEGDPDADGQAST"
     gene            3342..>3549
                     /locus_tag="AC159P_02485"
     CDS             3342..>3549
                     /locus_tag="AC159P_02485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694351.1"
                     /translation="MAKPRHNFHPRCGLSLTRLHGTGIARIGRVAESARGVEARFGGV
                     LCPMSCVSDHVENKTQVLVIDDEPS"
BASE COUNT          640 a         1206 c         1205 g          498 t
ORIGIN      
        1 aagccgagaa ggagcagatc cggcaccagt tgttccagtc gtcgaagctg gcctcggtcg
       61 gcgagctgtc ggcgggcgtc gcgcacgaga tcaacaaccc gctcaacggc atcatcaact
      121 tcgcccaact gctgaaggac gacgaggtgg cgcgcaccga cgggcagcgg cgcatggtgg
      181 acggcatcat cgacgagggc gagcgcatcg cccgcatcgt gcgcaacctg ctcaccttcg
      241 cgcggcagga cccgcacgag ctgacccgcg tcaacatcgc cgagaccatc gacgcctcgc
      301 tctcgctctt cggccaccaa ctgcggaagg acggcatcgc ggtcgagatc gcggtcgagg
      361 agaacctgcc gccggtgatg gccgacggcc cgcgcctgcg gcaggtgatg gtcaacatgg
      421 tgagcaacgc gcaccacgcg ctgcgcgcga aggcgggggg cgagaaggtg ttccgcacga
      481 cggcgcgcgc cgtcgagacg gcgggcggcc ccgccgtgcg gatcgagttt tacgacacgg
      541 gcgtcggcat ccggcgcgag gacatcgaca aggtcttcga cccgttcttc acgacgcggc
      601 gggccacggg cgggacgggc ctcggcctca gcctcagctt cggcatcgtc cgcgaatacg
      661 gcgggacgat cacggtcgag agtgccgaag gcgacttcac ccgcttctgc gtcgagctgc
      721 ccgccgcaga acttcgagag gtcaagcatg acgagcgcgc cgagagtgct gttggtggac
      781 gatgagccga acatgcgctg gacgatggcg gagtttttga ggcgcggcgg ctacgagccg
      841 ctgaccgcct ccgacttcga gagcgcgacc gagctgctcc gcgacgccga ggtggacgcc
      901 gccgtggtgg acatcgtcct gcccggcaaa agcggcgtcg agctgctcaa ggagctgcgg
      961 ggccgcgagc cttacgtccc ggtcgtcatg attacgggcg agcctaactc cgtactggtg
     1021 ccggagatcg tgcgcgcggg cgcctacgac tacatcgcca agccgattgt gaaagacgcg
     1081 ctcatcaagg cggtcgcgcg cgccgtcgag cgcaagcggc tcgaagacga aaagcgccgg
     1141 ctcgaacggg agatcaagca ccacgccgag gagctggaag cgcgcgtcgc cgagcgcacg
     1201 gcgcagctcg tcgaggcgca cgaacggctc gcgcatcagg agaagatcgc ggccctcggt
     1261 cgcgccgccg cgcaggtcgc gcacgaggtg aagaacccgc tcgcggggct gctgctctac
     1321 gcgatgcact tgaagacgaa ggccgccggc aagctcgcgg agggcgagct ggcgctcgtg
     1381 gacaagatcg tcgagacgat caaccacctc accgacaccg tcgagcaggt gctcaacttc
     1441 gcgcgccccg tctgcgtcac gccgcgcccc ggcgacctca accgcgtcgt ctcggatgtg
     1501 ctccaactcg cacagccgca gatggcgggc gggctgatcg agcagcgcct cgaactcgac
     1561 gagcgcggct gccgcgggat gctggacgag acggcgctgc gcggagcgtt gatgaatctg
     1621 gtgctgaacg ccgtgcaggc gatgcccggc ggcggcactt tgtcggtgag aacggcggcg
     1681 gacggcgcgg cgctgcgcct ggaggtcgcc gacacgggtt gcgggatgac cgaggagcag
     1741 atcggccacg tcttcgagcc gttctacacg acgaagagtc agggcctcgg cctcggcatg
     1801 ccgtacgcgc agaaggtgat cgagcagcac cgcgggacgg tctgcatcga gagccgaccg
     1861 ggcgcgggca cgaccatccg cgtcgagctt ccggcggaga ccggggcggg ctgaaaactt
     1921 cacacacgac agaggtgggc cgaagatgcc gaggtcgagc agaatccttg tggtggacga
     1981 cgaggcgagc gtcaccggcg ctcttgagct ggtgctcggc gaccgcggcc acgtagtcgc
     2041 gtcggcgcgc agcgtcggcg ccgcgtccga gcttctcaaa ggcgacccct tcgacctcgt
     2101 cttcacggac ctgcgcctgc ccgacggcag cggcatcgac ctgctgacca gcatcaagcg
     2161 cgacgcgccc gacatcgagg tcgtcctgat gaccgcgcac ggctcgctcg acgtgaccat
     2221 cgaggccatc aagcgcggcg ccttctacta cctcgaaaag cccttcacgc ccgaccaggc
     2281 gctcgtgctg gccgcgcgcg ccctgcagtt caaggcgatg aagaacgaga accgcgtgct
     2341 gcgggccacg cgcccgtcgg acggcgaaga cttcggcctg ctcggtcgtc acccgaagct
     2401 gcggcagatt cacgaggtca tccgcaccgc cgcgccttcg gacgcctcgg tgctcatcga
     2461 gggcgagtcg ggcacgggca aggagctgat cgccgcggcg cttcatctta agagcgagcg
     2521 cgccgcccgc cccttcatcc gcatcaactg cgcggccatc ccctcggagc tgatcgagtc
     2581 ggagctgttc ggctaccgga agggggcttt cacgggcgcc gaccgcgaca agcgcggact
     2641 cgtcgaggcg tcggcgggcg ggacgctctt gctcgacgag atcgccgaga tgcccgcgca
     2701 cttgcagacg aagctgctgc gcgtgcttca ggagcggcgc ctgcgacgcc tcggcgacga
     2761 gcacgaggtg gtcgtcgatt tccgcctcat ctcttcgacc aaccgcgaca cggcgcagat
     2821 gattcaggag ggcgcgctgc gcaaagacct ctacttccgc atcagcacga tcaaggtgaa
     2881 agtaccccct ctgcgcgagc ggcttgacga cgtgatgctg ctcgccgagc acttcctgca
     2941 acgctacgcg gagaagtacc gcaagcgcgt gcgcggcctc tcgcaggcgg cctactctct
     3001 gctcgcgcgc tacgattggc cgggcaacgt ccgcgagctg gaaggcgtcg tcgagcacgc
     3061 cgtcctcttc gcgcaggacg aacagctcat gcccgaagac ctgcccgaac atctccacgc
     3121 cgcgcaggcc acacacttcc gctgcgtcat cccgccctac ctcacgatgg aggagatcga
     3181 gcgcgaggcc atcgcgcaga cgctcgaacg caccggcggc aacgtcaaga agaccgcgca
     3241 gattctcgac taccaccgcc ccacgctcta ccgtaagctc cgccggttcg gcctcagaga
     3301 cgcgggcgac gacaaaaacg ccgaaggcga ccccgacgcc gatggccaag cctcgacata
     3361 atttccaccc gcgctgcggc ctgagcctga ctcgccttca cggaacgggg attgcacgca
     3421 taggacgcgt cgccgagtcc gcccgcggag tcgaggcgcg ctttggggga gtcttgtgtc
     3481 cgatgtcgtg cgtgagcgat cacgtggaaa ataaaaccca agttctcgtc atcgacgacg
     3541 agccgagcg
//
LOCUS       DBNGOH010000134         5600 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2768017, whole genome shotgun sequence.
ACCESSION   DBNGOH010000134 DBNGOH010000000
VERSION     DBNGOH010000134.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5600)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5600)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5600
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2768017"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..510)
                     /locus_tag="AC159P_02490"
     CDS             complement(<1..510)
                     /locus_tag="AC159P_02490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694352.1"
                     /translation="MSRTSKTPRRAILVVSALILSAGVLALPGRLALLTMPTAQAAGF
                     TWTGAVSSDWFTAGNWSPAGVPGAGDTATVSSGTVNVPTDASVGGLNFSNGIIQGAGT
                     LTITGTLNWTGGVMRGPGTTVIAAGATVSAPPTNNERILEGGRVFNNAGTFNCSGGPG
                     GRCVLAQGSG"
     gene            complement(573..854)
                     /locus_tag="AC159P_02495"
     CDS             complement(573..854)
                     /locus_tag="AC159P_02495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694353.1"
                     /translation="MKTLKRTLILQETHEVLSVRRLVYASQRRAPAWCEACAGEVPLL
                     TPEEAALATGVGARAIYRLVEAGQIHFEETPDGLLLVCPNKLKERSLDV"
     gene            1121..3085
                     /locus_tag="AC159P_02500"
     CDS             1121..3085
                     /locus_tag="AC159P_02500"
                     /inference="COORDINATES: protein motif:HMM:NF012698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HIR3694354.1"
                     /translation="MDASPTILYEFGPFRLDPRLPLLLRDGEPVPLQPKALETLVALV
                     ERGGQVVSREELVAAVWPDAEVEENNLSVNVSLLRKALGEAGGGVRYIETVPRRGYRF
                     TAPVSERPAESHELLYTRHTRSQILIEESQEGDAPPYAVAPAPAPQVTLEASPAVAAV
                     KSRGRSAFVAASVILLLAIGGLGVRYLGNRSSNAAQIESIAVLPFDNESADADVEYLS
                     DGMTESLINSLSQLPNLNVKARSSVFRYKGKDVEPEQAGNELSVQAVLTGRVVQHGDE
                     LMLSLELVDARTGNQIWGERYGRKLTDLVSLQSEIARDVAIKLRARLSGADEQRVTRR
                     YTDNALAYQLYLKGNFYASQYTKEGLSKGIAYFNQAIAADPDYALAYNGLAYYYIVAV
                     DWYLSPKDSMPKAREAARRALAIDESLADAHASLAVVLYWYDWDWAAAEREYRRAIEL
                     NPSDARVRQFYSVFLAHMGRSDEAIAEAERAQQLDPISLGANTYIGIILLFADREDEA
                     VEQLRKTVQMDSTFWLAHAFLGRAFEQKGDINQAMAEYRRARQLEENVPELLALVGRA
                     YALSGKKAEARKVIEELKELSNGTHVPPYNFAIIYAGLGNKDEAFAWLDRAYTERSYY
                     LTWLKVDPQMDGLRGDQRFHDLLRRVGLTP"
     gene            complement(3146..3592)
                     /locus_tag="AC159P_02505"
                     /pseudo
     CDS             complement(3146..3592)
                     /locus_tag="AC159P_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020676302.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            3918..5027
                     /locus_tag="AC159P_02510"
                     /pseudo
     CDS             3918..5027
                     /locus_tag="AC159P_02510"
                     /inference="COORDINATES: protein motif:HMM:NF012464.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(5472..5585)
                     /locus_tag="AC159P_02515"
     CDS             complement(5472..5585)
                     /locus_tag="AC159P_02515"
                     /inference="COORDINATES: protein motif:HMM:NF013068.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694355.1"
                     /translation="MEAAFPQAQVQLCLVHTIRTQPAIRFSSPSLVCRLAA"
BASE COUNT         1022 a         1790 c         1823 g          965 t
ORIGIN      
        1 gccgctgccc tgcgcgagga cgcacctgcc gccggggccg ccggagcagt tgaacgtccc
       61 ggcgttgttg aagacgcgcc cgccttcgag gatgcgctcg ttgttcgtgg gaggcgcact
      121 gacggtcgcg ccggcggcga tgacggtcgt gcccggtccc ctcatcacgc cgcccgtcca
      181 gttgagcgta ccggtgatgg tgagcgtgcc cgcgccttga ataatgccgt tgctaaagtt
      241 gaggccaccg acgctcgcgt cggtcgggac gttgaccgtc ccgctgctga ccgtcgccgt
      301 gtcgccggcg ccggggacgc ccgcgggcga ccagttgccg gcggtgaacc agtcggagga
      361 gaccgcgccg gtccacgtga agccggcggc ctgcgccgtc ggcatcgtca gcagcgcgag
      421 tcgccccggc agggcgagta cgcccgccga cagaatcagc gctgaaacta cgaggatggc
      481 gcgtctgggg gtcttacttg tacggctcat atttttactc ctgagggcgc ggatgccgcg
      541 ggcggctgcg gcgtgtcgag cgtcggcccc ggtcaaacgt cgaggcttcg ctctttgagt
      601 ttattggggc agacgagcag cagcccgtcc ggagtctctt cgaagtgaat ctggccggct
      661 tcgaccaggc ggtagatggc gcgcgccccc acccccgtgg cgagcgccgc ttcttcgggc
      721 gtgagcagcg ggacctcccc cgcgcacgcc tcgcaccacg cgggcgcccg gcgctgagac
      781 gcgtagacga ggcggcggac gctcagcacc tcgtgcgtct cctggagaat cagtgtcctc
      841 tttagagtct tcatccgggg aactccggta gccgccggcg gtcgttaaag tctggcggga
      901 gggggaacct ccccccgcct cgggtaaggg agaatttttt tgccccggcg cgccccgtcg
      961 gctagaatgg gcagccgctc aaggccagcc cgccgcagac cgcgagtcac gccgccggag
     1021 cgccgtgaca tctacccgaa gaccgggcga aaagtccttt ggactcgctt gtttatttct
     1081 tgggattttc tttgggcggt tttccgaggg tttttaccat atggacgcct cgccgacgat
     1141 cctttacgag ttcggcccct tccgcctcga cccccgcctc cccctccttt tacgcgacgg
     1201 cgagcccgtc ccgctgcagc cgaaggccct ggagacgctc gtcgcgctgg tcgagcgggg
     1261 cggccaggtg gttagccgcg aggagctggt ggcggccgtc tggcccgacg ccgaggtcga
     1321 ggagaacaac ctctcggtca acgtctcgct cctgcggaag gcgctgggcg aggcgggggg
     1381 cggggtgagg tacatcgaga cggtcccgcg gcgcggctac cgcttcacgg cgccggtcag
     1441 cgagcgcccc gccgagagcc acgagctgct ctacacgcgg cacacgcgct cgcaaatcct
     1501 catcgaggag agccaagaag gggacgcgcc gccgtacgcg gtcgcgccag ccccggcgcc
     1561 gcaggtgacg ctggaggctt cgcccgccgt ggccgccgtc aaaagccgcg gccgctccgc
     1621 tttcgtcgcc gcgtcggtga tcttgttgct ggcaatcggc gggttaggcg ttaggtatct
     1681 cggcaaccgt tcttcaaacg ccgcgcagat cgaatccatc gccgtgctgc cgttcgacaa
     1741 cgagagcgcc gacgccgacg tcgaatatct ctcggacggg atgaccgaat cgctcatcaa
     1801 cagtttgtcg caactgccga atttgaatgt caaagcgcga agttccgtct tccgctacaa
     1861 aggtaaagac gtcgaaccgg aacaggccgg caacgaattg tctgtccagg ccgtcctgac
     1921 cgggcgcgtc gtgcagcacg gcgacgaatt aatgctcagc ctggaactgg tggatgcgcg
     1981 gacgggcaat cagatttggg gcgaacggta tggtcgaaaa ctgaccgacc ttgtctcact
     2041 gcaaagcgag atcgcgcgag acgtcgcaat caagttgcgg gcgcggcttt cgggcgcgga
     2101 tgagcaaaga gtaacgaggc gctataccga caacgcgctg gcttatcagc tctatctgaa
     2161 aggcaatttt tacgcctccc aatacaccaa agaaggttta agtaaaggga tagcgtactt
     2221 caaccaggcg attgccgcgg acccggacta cgctttggca tataacggtc tggcttacta
     2281 ctacatcgtg gcggtggact ggtacttatc gcctaaagat tcaatgccga aagccagaga
     2341 ggcggcgaga cgcgcgctgg caattgacga atcgctcgcc gacgcccacg cctcgctggc
     2401 cgtggtttta tactggtacg actgggactg ggcggcggcg gaaagagagt accggcgggc
     2461 catcgagctt aatccgagtg atgcgagggt gcgccagttc tactctgtat tcctggcgca
     2521 tatggggcgc tccgacgaag ccatcgcaga agcagagcgg gcgcagcagc tagacccgat
     2581 ctcgctgggg gctaatacat acatcgggat aattctactt ttcgcggata gggaagacga
     2641 agcggtcgaa caactgcgaa agaccgtcca gatggattcg actttttggc tcgcgcacgc
     2701 ctttttgggg cgcgcctttg agcaaaaagg cgatattaac caggcgatgg cggagtaccg
     2761 gcgggcgaga cagttggaag aaaacgtccc ggaattactg gcactggtcg gacgcgctta
     2821 tgcgctctcg ggcaaaaaag cagaagcccg aaaggtcatc gaggaattga aagagctttc
     2881 aaatggaaca catgttccgc cctacaactt cgccataatt tacgccgggc tggggaataa
     2941 agatgaagcg ttcgcctggc tcgacagggc ttacacggaa cgctcgtact atctcacttg
     3001 gctcaaagtg gacccgcaga tggacggcct acgcggcgac cagcgctttc atgatttgct
     3061 gcggcgcgtc ggcctcacgc cctagtggaa ctgagttcca ccgggctaaa ggagagtgtc
     3121 cagagttttg tgtaagtgat cgtcactaga gaacaggcat cctgtcctcg aagatgatgg
     3181 cgaagcggtt cagcgccgcc ttccactcct tgctcggcat cgtccatttc cgcgcgatgt
     3241 tctgcaacgc caggtagagc agtttcagcg ccgcctcatc cgaagggaac gagccccgat
     3301 tttgaatgat cttccgcaga ctcatgttga ccgactcgat ggcgttggtc gtgtagatga
     3361 ctttgcggat gtccgccggg taagcaaaga acggagtgat gcgctcccaa ctcgctcgcc
     3421 acgaccggct gatcgtcggg aactgggggt cccacttttc ggcgaactcc gagagcctga
     3481 gttcggcctc ttcgcgcgtc gccgccctgt agatcgtctg taagtcagag gccacttcct
     3541 tgcgctgctt ccagccgacg tagttcaagg agtgcctgac cagatgcacc atccgaacgc
     3601 aatcgactca ttgcgagcca cactggtcat ccctttcgta tccaccatca agccccacgt
     3661 ccggcgtcgc cgcccgtagc cgcggcatgc tccggcctga catcctcgga gccctcggag
     3721 gttccaacag atgtcacaac acagtcgtgc ccgctgcccg gtccagtggg ggatcacgtc
     3781 ggtgagcgtc aggacgcgcg atgcccgaca ccgggtcgcg caagcctacc gcatcgtcct
     3841 cgggtgtggc gacgagtcgg ccgcatgcga ggcgagtccc aacaggctgc ccggtgccgc
     3901 cacgaagggg gagagagatg agagccgcca tctatgccag agtctccacg gatcgccaac
     3961 aacagaacca gaccattgac tcgcaactcg ccgcgctccg gtcatgggtc ggcagccacg
     4021 gccatgagtt gctgccgggg cacgtcttca ccgacgaggg ctacagcggc tcgcgcctcg
     4081 accgtcccgg cctcgacgcc ctgcgcgacg cggcgcacga cgcctcgttc gacctcgtcg
     4141 ccgtcctctg ccccgaccgc ctcgcccgca agtacgctta ccaggttctg ctccttgagg
     4201 agctgcgacg cgccggctgt gaggtggcct tcctccagca cccgatctcg gatgatccca
     4261 acgaccagct cctgttgcag atccagggtg ccatcgccga gtacgagcgg gcggtgctgg
     4321 gcgaacggtt ccgtcgcggc aagctgcaga aggcgcgtga cggccattac caggcgggga
     4381 aggctcccta cggctaccgc tacctcccga agcgggaggg cgagcccggg cacctcgtcg
     4441 tcgatgaggc tgaggcgggg gtggtccgca ccatctacgg ctggctcgtc gaggaacgca
     4501 tgacggtgcg ccagatcctc aagcggttga acgcggggcc gtggcttccg cgctcgggcc
     4561 gtcatcaatg gtccaacgcc gtcgtccacc acatcctcgc cgacccggtc tacgccggca
     4621 ccgcctacgc caaccgctac cgcggcgtcc cacccaagcg cccgcgccgg cgcggccccc
     4681 gcacggccga gaactccgcc cgccaactca ggccgcgtga ggagtgggtc gccatcccgg
     4741 tgccgccgct cgtcacgcgc gagaccttcg agctggcggc ggcgcaactg gcccgcaact
     4801 ccgtcctctc gttccgtaac aacgccaggt acagctacct gctgcgctgc ctgttgacgt
     4861 gccggagttg cagcctggcg atgttcggca tcacccggcc ggcgagcgct aaacaaccgt
     4921 tgcgcagcta ctacacctgt cacggtaaag acggtgtgat gagcgcccgc ccgcacccct
     4981 gcccacgccg ccccgtcaaa gccgcggagt tggaggcggc ggtctgggat cacgtcaagc
     5041 gattactctc tgacccggag caactcctgg cccagttccg cgactacgcg cagatggcga
     5101 cggaaggtca gacgagacag cagtccgagg cggagaagtt agaggcgcgc gcggcgcgcc
     5161 tggcgcgtga agagaagcga ctggttgacg cttatcaggc ggaagtgatc agcctggagg
     5221 aactggccga gcgccggcgc caactcaccg gcagacgccg tgcgctcgac gcgcagcggg
     5281 agcagtacgc gaagctgcgg caggagagcc ggcacgcgcg gcaggtgctc ggagacctga
     5341 gcgccttctg cgcacgagtg cggaagcggc tcgacgaggc gaccttcgcg gagaggcagg
     5401 cgatcttgca gatgctgatc gagcggatta tcgttgggga ggatacgctg gaagtccggc
     5461 atgtgatccc gctacgcggc gaggcgacag acaagggagg ggctgctgaa ccgaatggca
     5521 ggttgcgttc ggatggtgtg gacgaggcag agctgcacct gcgcctgcgg gaaggccgcc
     5581 tcgatcgcct cggggaagcc
//
LOCUS       DBNGOH010000135         5377 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2218241, whole genome shotgun sequence.
ACCESSION   DBNGOH010000135 DBNGOH010000000
VERSION     DBNGOH010000135.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5377)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5377)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5377
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2218241"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            255..908
                     /locus_tag="AC159P_02520"
     CDS             255..908
                     /locus_tag="AC159P_02520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HIR3694356.1"
                     /translation="MFQEMEEARKTVVSLNETFAIVSLGGFAIQRAIEIIDPFFVALL
                     FAVKENREKKDLPFGMSEKDVKTWLAASIGFLIGLALTFSTDMRLPEVGFEWGDWDKI
                     ILALALSAGANGLNSMLKFGEHVKEARKAEVRPLPTIRISPALPVVKQGSTIKLLASV
                     SGTDIKDVRWEVLEANGGTIVPDDGTYTAPAQPGRFHVAAISQANEEAFATVTVTVK"
     gene            complement(949..2316)
                     /locus_tag="AC159P_02525"
     CDS             complement(949..2316)
                     /locus_tag="AC159P_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012531714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HIR3694357.1"
                     /translation="MAETVLIVDDERGIRETLRGVLEDEGFAAEAVASGEECLKAVER
                     RAYGCVLLDVWLPGLDGLQTLERLRERGSEAAVVMISGHATIETAVRATKLGAFDFIE
                     KPLSIEKTVLTVRNALRQRELERVNAALNEELREEWVMVGESVAMRALRKQIALVAPT
                     DGRVLIYGESGTGKELVARAIHAGSRRARAPFVELNCSAVPEELIESELFGHARGAFT
                     GATAAKRGKFELADGATLFLDEVGDMSARMQAKVLRVLEEQRFEPVGAPTPMRVDVRV
                     IAATNKRLEGEIERGHFRADLLFRLNVIPFELPPLRERTEDIPLLVEHFNRKFSLAYG
                     KQPKRFEPDAVEALQNYSWPGNVRELRNTVERVVIMHPRLKVAAADLPALGGEEPPAA
                     PPRFSTFRAAGEAHQREFIKRKLAEAEGNVSRAAELMGMDRSHLYRRMRALGINVREE
                     RAGAS"
     gene            complement(2316..4703)
                     /locus_tag="AC159P_02530"
     CDS             complement(2316..4703)
                     /locus_tag="AC159P_02530"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3694358.1"
                     /translation="MEEKATEANGAAADAARVQKRSAPWLYGGLVLLLLSVLVVLQVF
                     GLWEVFTPDTASDTLLLYALSSLNFAAFFVFTFIFIRSLLKLRRERRERLLGSKLKTR
                     LVVYFIAVSLLPITAMALFSYIFFNRTLEKWFGRVPEDIVLEAKYARDEYLAQQAQSL
                     RESAGLAALALERQSAEAAPTELGNLLTRGDFAAVALAAPEGILEDRLRWREGVDFKQ
                     SKDSAGAMKTALWRLHNGAGERLPVTTTDGFIADALPLSNGLTLLVAREVRGDTRLDR
                     IAAGAATFEQLRRQQRKVRLLGLSTLGLLTLMLLFGATWSAIHLARGIGAPIRALAEA
                     ADEVAAGNLDHRVDAIADDELAVLAASFNDMTAQLAENRRRIERNATELSDKNLALEE
                     RRNYIETVLQTLSTGVVSLDGANRVTTINAAARSMLRLERATAQRVAAAPLSELIGAE
                     DFAVIERVLWRARLRGRASEQVELARGTSSPEQPHDEGANGESGANGESGAGLIPVAL
                     TATALAPRAGETKRGVVLVIEDLSELLAAQRAAAWSEVARRLAHEIKNPLTPIQLSAE
                     RIARNFRRAAEGNGDADARGGETSSRDGNGKGGERERVARVVEECTATISREVAGLKA
                     MVDEFTRFARLPHARLAPADLNEVVRQSVALYEDRLEGVRLDVLLAPRLPAAMLDAEQ
                     MRRVFVNLIDNSIEAFDADGAERRITVATGHDPARGLLIAEVADTGHGIARADFARLF
                     QPYFSTRGRETGLGLAIVHRIMAEHGGRIRAEQNRPRGARMLIELPVADEARGAE"
     gene            4984..>5377
                     /locus_tag="AC159P_02535"
     CDS             4984..>5377
                     /locus_tag="AC159P_02535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694359.1"
                     /translation="MNEPVGIFFNPAGRLRSVLRFVVFCFAFLVAFVALFLFVRFSMA
                     LALSPAANGAFFESNWEFVLQGVLILLVASLVGWLCGYALEDLPWRALGWALHRGWVR
                     DLLVGSLLGAASMGVAVVIDIAVGGTRFA"
BASE COUNT          766 a         1860 c         1875 g          876 t
ORIGIN      
        1 ccaacgccac cgagttggtc aaggaagacc acgtgcgcgc caacttcggc ttctcgctcg
       61 acacggacaa gctgttcacg gtcatcagaa ccctcggcgt ggggcgcgtg cagtaagacc
      121 gcgctcacgc cgcgcgatgc gctacccctc gcggaacggc ggcggggcgg ctcggcccgc
      181 acgaagggtt gaagccgccc gccgccccga acaccaacgc cgggcgaccg gcctcttgcc
      241 ctgacaggtg acttatgttc caagaaatgg aagaggcccg caaaacggta gtcagtctca
      301 acgagacttt cgccatcgtc agcctcggcg gcttcgccat ccagcgcgcc attgagatca
      361 tcgacccgtt cttcgtcgcg ctgctcttcg ccgtcaaaga gaatcgggag aaaaaagacc
      421 tccccttcgg catgtcggag aaggacgtga agacgtggct cgccgcgtcg ataggtttcc
      481 tcatcggcct cgccctcacc ttctcgacgg acatgaggct tcccgaagtc ggttttgaat
      541 ggggagactg ggacaaaatc attctcgcgc tggcgctcag cgcgggggcg aacggcctca
      601 acagcatgct gaagttcggc gagcacgtga aggaggcgcg gaaggccgaa gtgaggccgc
      661 tgcccaccat cagaatctcg ccggccctcc ccgtcgtcaa gcaaggctcg acgatcaagc
      721 tgctggccag cgtcagcggc acggacatca aggacgtgcg gtgggaggtg ctggaggcga
      781 acggcgggac gatagtcccg gacgatggga cgtacacggc ccccgcgcaa cccggcaggt
      841 ttcacgtcgc cgcaatcagc caagccaacg aagaggcttt cgccaccgtg accgtcaccg
      901 tcaagtagcc tgcccgccgc ccgacctcgc tccggctgcg gccccgcctc aggaggcgcc
      961 cgcgcgctct tcgcggacgt tgatgccgag ggcgcgcatg cggcggtaga ggtggctgcg
     1021 atccatgccc atcagctcgg cggcgcggga gacgttgcct tcggcctcgg cgagtttgcg
     1081 tttgatgaat tcgcgttggt gggcctcgcc ggcggcgcgg aaggtggaga agcgcggggg
     1141 cgccgcgggc ggctcctcgc cgccgagcgc gggcaggtcg gcagccgcga ctttgaggcg
     1201 cgggtgcatg atgacgacgc gctcgaccgt gttgcgcagc tcccgcacgt tgccgggcca
     1261 ggagtagttt tgcagcgcct cgacggcgtc cggctcgaag cgcttgggct gcttgccgta
     1321 ggcgagcgag aacttgcggt tgaagtgttc gacgaggagc gggatgtctt cggtgcgctc
     1381 gcgcaagggc ggcagctcga aggggatgac gttgaggcgg aagaggaggt cggcgcggaa
     1441 gtggccgcgc tcgatctcgc cttcgagccg cttgttggtg gcggcgatga cgcgcacgtc
     1501 cacgcgcatc ggcgtcgggg cgcccacggg ttcgaagcgc tgctcttcca gcacgcgaag
     1561 gactttcgcc tgcatccgcg cgctcatgtc gccgacctcg tcgaggaaga gcgtcgcgcc
     1621 gtcggccagc tcgaacttgc cgcgcttggc cgcggtggcg ccggtgaagg cgccgcgggc
     1681 gtggccgaac agctccgact cgatcagctc ctcggggacg gcggagcagt tcagctcgac
     1741 gaagggggcg cgcgcgcggc gcgagccggc atggatggcg cgggcgacca gctccttgcc
     1801 ggtgcccgac tcgccgtaga tgagcacgcg cccgtcggtc ggcgcgacca gcgcgatctg
     1861 cttgcgcagg gcgcgcatcg ccacgctttc gccgaccatc acccactctt cgcgcagctc
     1921 ttcgttgagc gccgcgttga cgcgctccag ctcacgctgc cggagggcgt tcctgacggt
     1981 gagcacggtc ttctcgatgg agagcggctt ctcgatgaag tcgaaggcgc cgagcttggt
     2041 cgcgcgcacg gcggtctcga tggtggcgtg gcccgaaatc atgacgaccg ccgcctcact
     2101 cccccgctcg cgcaggcgct ccaacgtctg tagcccgtcg aggcccggca gccatacgtc
     2161 caagagcacg cagccgtacg cgcgccgctc gacggccttc aggcactcct cgcccgacgc
     2221 caccgcctcc gccgcgaagc cctcgtcttc gagcaccccg cgcagcgtct cgcggatgcc
     2281 acgctcgtcg tccacgatga gaacggtctc ggccatcact ctgcgccccg cgcctcgtcc
     2341 gcgacgggca gctcgatgag catgcgcgcg ccgcgggggc ggttctgctc ggcgcggatg
     2401 cgcccgccgt gctcggccat gatacggtgg acgatggcga ggccgaggcc ggtctcgcgc
     2461 ccgcgcgtcg agaagtaggg ctggaagagg cgcgcgaagt cggcgcgggc gatgccgtgg
     2521 ccggtgtctg cgacttcggc gatgaggagg ccgcgcgcgg ggtcgtggcc ggtggcgacg
     2581 gtgatgcggc gctcggcgcc gtcggcgtcg aacgcctcga tggagttgtc gatgaggttg
     2641 acgaagacgc ggcgcatctg ctcggcgtcg agcatggccg cgggcagccg cggcgccagc
     2701 agcacgtcga gccggacgcc ttcgaggcgg tcttcgtaga gcgcgaccga ctggcggacg
     2761 acttcgttga ggtcagcggg cgcgagccgc gcgtgcggca gccgcgcgaa gcgcgtgaac
     2821 tcgtccacca tcgccttcag gcccgccacc tcgcggctga tggtcgccgt gcactcctcg
     2881 acgacgcgcg cgacgcgttc gcgctcgccg cccttcccgt tcccatctct cgaactcgtt
     2941 tcgccgccgc gcgcgtcggc gtcgccgttc ccttccgcgg cgcggcggaa gttgcgcgcg
     3001 atgcgctcgg ctgagagctg aatcggcgtc aaagggttct tgatctcgtg cgcgaggcgg
     3061 cgcgcgacct cgctccacgc ggcggcgcgc tgcgcggcca gcagctccga gaggtcttcg
     3121 atgacgagca cgacgccgcg cttcgtctcg cccgcgcgcg gggcgagcgc cgtcgccgtg
     3181 agcgcgaccg ggatcaggcc cgcgccgctc tcgccgttcg cgccgctctc gccattcgcc
     3241 ccttcgtcgt gcggctgctc gggcgacgag gtgccgcgcg ccagctccac ctgctccgag
     3301 gcgcgaccgc gcaggcgcgc gcgccacagc acgcgctcga tgacggcgaa gtcctcggcc
     3361 ccgatcagct cggagagcgg cgcggcggcg acccgttgcg ccgtcgcgcg ttcgagccgg
     3421 agcatcgagc gcgcggcggc gttgatggtc gtcacgcggt tcgcaccgtc gagcgagacg
     3481 acgccggtcg agagcgtttg cagcacggtc tcgatgtagt tgcgccgctc ttcgagcgcg
     3541 aggttcttgt cgctcagctc cgtcgcgttg cgctcgatgc ggcggcggtt ctcggcgagc
     3601 tgcgccgtca tgtcgttgaa cgaggcggcg agcacggcca gctcgtcgtc ggcgatggca
     3661 tcgacgcgat gatcgaggtt gcccgcggcc acttcgtccg ccgcctccgc gagcgcgcgg
     3721 atcggcgcgc cgatgccgcg cgcgaggtgg atcgcgctcc acgtcgcgcc gaacagcagc
     3781 atcagcgtca gcagcccgag cgtcgagagg ccgagcagac gcaccttcct ctgctgccgg
     3841 cggagctgct cgaaggtggc ggcgcccgcg gcgatgcggt cgagccgcgt gtccccgcgc
     3901 acctcgcgcg cgacgagtag agtgagaccg ttcgagagcg gcagcgcgtc ggcgatgaag
     3961 ccatcggtgg tcgtgaccgg caggcgctcg cccgccccgt tgtgcagacg ccacagagcg
     4021 gtcttcatcg cgcccgccga atccttgctc tgcttgaagt caacgccctc gcgccagcgc
     4081 aaccgatctt cgaggatacc ctcgggcgcg gcgagggcca ccgccgcgaa gtcgccgcgc
     4141 gtgagcaggt ttccgagttc ggtcggcgcg gcttccgcgc tctggcgttc gagcgcgagg
     4201 gcggcgaggc ccgcgctctc gcgcaaactc tgcgcctgct gcgcgaggta ttcgtcgcgc
     4261 gcgtacttcg cttcgagcac gatgtcttcg ggcacgcgcc cgaaccactt ttcgagcgtg
     4321 cggttgaaga agatgtagga gaagagcgcc atcgccgtga tcgggagcag gctgacggcg
     4381 atgaagtaga cgacgagccg cgtcttcagc ttcgagccga ggaggcgttc gcgccgctcg
     4441 cgcctcagct tcaggagact gcggataaag atgaaggtga agacgaagaa ggccgcgaag
     4501 ttgagcgacg agagcgcgta gagcagcagc gtgtcgctcg ccgtgtcggg cgtgaagacc
     4561 tcccacagcc cgaagacctg caacacgacg agcaccgaga gcagcagcag caccagcccg
     4621 ccgtagagcc acggcgcgct ccttttttgc acgcgcgccg cgtccgccgc cgccccgttc
     4681 gcctctgtcg ccttctcttc catgcgagtc gtgccggagt ttatcacgcg ccgtcacgcg
     4741 gcacacagcc tgaccccatt cgcgccgtcc gccgcccggc ccgtgttatg tgatgggacg
     4801 cgggcgcccc ttccgctgtt tcgcgccgga acacgccgcg gccctcgggc cgcttggatt
     4861 cgacttccaa tccccggcgc gcggcggcgg cggccctgct tgcgcttcct ccgagtcgag
     4921 tgtatttatg ccccagcgac tttcagtccc agtctgcgac cgtcaagctc tcagcgaaca
     4981 cctatgaacg aaccggtcgg catcttcttc aacccggcgg gcaggctgcg gagcgttctg
     5041 cgcttcgtcg tcttctgttt cgcgttcctc gtcgccttcg tcgcgctctt cctgttcgtc
     5101 cgcttctcga tggcgctcgc gctcagcccc gccgccaacg gggctttctt cgagagcaac
     5161 tgggagttcg tccttcaggg cgtcttgatc ctcctcgtcg cctcgctcgt cggttggctc
     5221 tgcggttacg cgttggaaga cttgccgtgg cgcgcgctcg gctgggcgct gcaccgcggg
     5281 tgggtgcgcg acctcctcgt cgggtcgctg ctcggcgccg cttcgatggg cgtggcggtc
     5341 gtcatcgaca tcgcggtcgg cgggacgcgc ttcgcgc
//
LOCUS       DBNGOH010000136         3504 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1301992, whole genome shotgun sequence.
ACCESSION   DBNGOH010000136 DBNGOH010000000
VERSION     DBNGOH010000136.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3504)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3504)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3504
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1301992"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..385)
                     /locus_tag="AC159P_02540"
     CDS             complement(<1..385)
                     /locus_tag="AC159P_02540"
                     /inference="COORDINATES: protein motif:HMM:NF047043.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6916 family protein"
                     /protein_id="HIR3694360.1"
                     /translation="MKAGALSALAAGFALNSARLALAQTSTPSQDFPISQEAKADPVF
                     YYRKSTFDPYLNTTFRARARGGVVELVLVKITDSCSPARSALTRRSRVSQGFTLLFRA
                     SAPLSTITTIHTLDHDALGQFSLFLE"
     gene            1017..1517
                     /locus_tag="AC159P_02545"
     CDS             1017..1517
                     /locus_tag="AC159P_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010469716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_component: GO:0098024 - virus tail, fiber [Evidence
                     IEA];
                     GO_process: GO:0098003 - viral tail assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HIR3694361.1"
                     /translation="MSEPFLSEIKIVSFNFPPKGWALCNGQFLPINQNQALFALLGTT
                     YGGNGQTTFALPNLRGRVPIHFDSSHNLGEAAGSTSVTINQQTMPQHLHFMNASNQGG
                     NALTPSGNVLGAVAAKIYNDPGSLTTLAPTTVSSVGGSQPHNNMMPYLVLNFIIALQG
                     IFPSRN"
     gene            1536..2054
                     /locus_tag="AC159P_02550"
     CDS             1536..2054
                     /locus_tag="AC159P_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_component: GO:0098024 - virus tail, fiber [Evidence
                     IEA];
                     GO_process: GO:0098003 - viral tail assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HIR3694362.1"
                     /translation="MPEPYIGEIRMFAGNFAPNGWMFCEGQTLPISENDALFTLIGTT
                     YGGDGQETFILPNLASRVPIHMGTGPDGTTYQIGEMAGTEQETLTVQQIPSHTHTMTA
                     STAVAQQTDPTGRVVAQSAGGFLYIEDVPDVNFAANAISPAGGSQPHENCQPFLCINF
                     IISLFGVFPSQT"
     gene            2069..2599
                     /locus_tag="AC159P_02555"
     CDS             2069..2599
                     /locus_tag="AC159P_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_component: GO:0098024 - virus tail, fiber [Evidence
                     IEA];
                     GO_process: GO:0098003 - viral tail assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HIR3694363.1"
                     /translation="MSDQFVAEIRIFPFNFPPTGWAFCNGQLLPLSQNTALFSLLGTT
                     YGGDGKSTFALPNLQGNAPMQPGQGQGLSLRDLGEMSGVESVTLLVSEIPLHTHIASG
                     SESIATVQAPTPTVIMARMEGGANAYQSVTNANIVQMAPQELPPAGGSLPHNNMQPYL
                     TLNFCIALQGIFPQRP"
     gene            2774..>3504
                     /locus_tag="AC159P_02560"
     CDS             2774..>3504
                     /locus_tag="AC159P_02560"
                     /inference="COORDINATES: protein motif:HMM:NF037016.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HIR3694364.1"
                     /translation="MKHYTRSFTRRNVRAYFASLLSYLLLAGQMTPLVLAAKAPAPRV
                     APHTDVARESAGEVPPLQAAAAPAPLAAVVAPIISATKVDAFADPNMDGKADPGQTIS
                     YTVTITNTGPDPATNLTFTDTVDPNTTLQPGTVIASPIAVDESYAVLGNVRIQPNAAA
                     GLLANDVNPNTGNNTGLTISGPTTSAQNGNVVVNADGSFSYNPAPGFTGTDSFTYKSN
                     DGSLDSNVATVTIRVAPAGDTEGKVT"
BASE COUNT          664 a         1270 c         1002 g          568 t
ORIGIN      
        1 gttcgaggaa gagcgagaac tggccgagcg cgtcgtggtc gagcgtgtgg atggtggtga
       61 tggtggacag cggggccgac gcgcggaaca gcagcgtgaa cccctgcgag acccgcgacc
      121 tcctcgtcag cgcgctgcgc gcgggcgagc aactgtcggt gattttgacg agcacgagtt
      181 cgaccacgcc gccccgcgcg cgcgcgcgga aggtcgtatt caggtatggg tcgaacgtcg
      241 atttccggta gtagaagacc gggtcggcct tagcctcctg ggagatggga aagtcttgcg
      301 agggggtgga agtctgtgcg agggcgaggc gggcggagtt cagggcaaat cccgcggcca
      361 gggcagagag cgcccccgct ttcatgaagc ttcttcttgt gacgggcatc ttcggttttt
      421 ctcctaagca ataggtaggt gggaggcagg ccccttcaag cccgccgctt tgcagcgtat
      481 cttagtcggg cgcgggatga caatacattt ctgcgaaggc ccccggcgcc tcggtcgtgg
      541 cgctcggcgc tccgcaaacc cctcgccgga gtatcctgta acataaaccg tccgcatcgg
      601 ccctcgttct ccctccggga atcccgtgcc gggccccacc cgcgcaccat ataaataccg
      661 aatgtgcccc cccgccgtca ccacctagac aggtagcgcc gtgaggggcg gcacgctctg
      721 gccggccaac ggtaaagcga ggaggcgtca gctttttctt gatattctgc ggtcctgcgt
      781 ttacaatctc ccgcgcttcg ctgtttggac aaagcttttc ggctcctcgt tggcaaggca
      841 tcccaaggca agtctttacc cagcacactg gcccgactct cgcagccctt gttcttagtc
      901 tttctcaagc actcttgact cacctgtccg gcgggtcgcg ccggatcgga cgacccggta
      961 agcggagtcg ttgataaccg ctcacgcaac ctacaaactc tctaaaggag tagaccatgt
     1021 ccgaaccgtt cctcagtgag ataaagatcg tctccttcaa cttccctccc aaaggctggg
     1081 cgctttgcaa cggtcaattc ttgcccatca accagaacca ggccctcttc gcgctgctcg
     1141 gtacgaccta cggcggcaac gggcagacca ccttcgcgct gccgaacctg cgcgggcggg
     1201 tgcccatcca cttcgacagc agccacaacc tcggcgaggc cgcgggcagc accagcgtca
     1261 ccatcaatca gcagacgatg ccgcagcacc tgcatttcat gaacgcctcg aaccagggcg
     1321 gcaacgccct gaccccttcg gggaacgtgc tcggcgcggt cgcggccaag atttacaacg
     1381 accccggctc actcacgacg ctggccccga cgaccgtctc cagcgtcggc gggagccagc
     1441 cccacaacaa catgatgccg tacctcgtgc tgaacttcat cattgccctg caaggcatct
     1501 tcccgtcgcg gaactagaag gaggacgaac acaccatgcc agaaccttat atcggagaga
     1561 tcaggatgtt cgccggtaac ttcgccccca acgggtggat gttctgcgag ggccagacgc
     1621 ttcccatctc ggagaacgac gcgctcttca cgctcatcgg cacgacctac ggcggcgacg
     1681 gccaggagac cttcatcctg ccgaacctcg ccagccgcgt ccccatccac atgggcaccg
     1741 gcccggacgg cacgacctac cagatcggcg agatggccgg caccgagcag gagacgctga
     1801 cggtgcagca gatcccgagc cacacccaca cgatgacggc cagcaccgcc gtcgcccagc
     1861 agacagaccc caccgggcgc gtggtcgcgc agtcggcggg cggcttcctc tacatcgaag
     1921 acgtgccgga cgtcaacttc gccgccaacg cgatctcccc cgccgggggc agccagccgc
     1981 acgagaactg ccagccgttc ctgtgcatca acttcatcat ctcgctcttc ggggtgttcc
     2041 ccagccagac ctaaaggaga agaagaaaat gtctgaccag ttcgtagccg aaatccgcat
     2101 cttccccttc aacttccccc cgacgggctg ggccttctgc aacgggcaac tgctgccgct
     2161 ctcgcagaac acggcgctct tctcattgct cggcaccacc tacggcggcg acggcaagtc
     2221 caccttcgcg ctgcccaacc tgcaaggcaa cgcgcccatg cagcccggcc agggccaggg
     2281 cctctcgctg cgcgacctcg gcgagatgtc gggcgtcgag agcgtcacgc tgctcgtgtc
     2341 cgagataccg ctccacacgc acatcgcctc ggggtccgag tccatcgcca cggtgcaggc
     2401 gccgacgccg acggtgatca tggcgaggat ggaaggcggc gccaacgcct accagtccgt
     2461 caccaacgcg aacatcgtcc agatggcccc gcaggagctg ccgccggcgg gcggctcgct
     2521 cccgcacaac aacatgcagc cttacctgac gctgaacttc tgcatcgcgc ttcagggcat
     2581 cttcccgcag cgcccgtagc acgttcctcg tcaccttccg cgcgggcggc ccactttccg
     2641 cgaagtgccg cccgcgcctc gctctctcga caatccggtt aatgacgaat gagcattctc
     2701 ccctcctcac gcgtcagcgt tgactccagc gcgcccgccg cggcggcgcc ctcccaaacc
     2761 atcaaggaga gttatgaagc actacacccg gtcgttcacc cgtcgcaatg tcagggccta
     2821 cttcgccagc ctcctctcgt acctgctgct cgcgggacag atgacgccgc tcgtgctcgc
     2881 cgccaaagct ccggcgccac gagtcgcccc acacaccgac gttgcgcgtg agagcgcggg
     2941 tgaggtgccg ccgttgcagg cggctgccgc accggcgccg ctcgccgccg tcgtggcacc
     3001 gatcatctcc gccaccaagg tggacgcctt cgccgacccc aacatggacg gcaaggccga
     3061 ccccggccag accatcagct acaccgtcac catcaccaac accggccccg acccggccac
     3121 gaacctgacc ttcaccgaca cggtcgatcc gaacacgacc ctccagcccg gcacggtcat
     3181 cgcctcaccc atcgccgttg acgagagtta cgccgtcctc ggcaacgtcc gcatccagcc
     3241 gaacgccgcg gcgggcctgc tcgccaacga cgtgaacccg aacacgggca acaacacggg
     3301 gctgacgatt tcggggccga ccacttcggc ccagaacggc aacgtcgtcg tcaacgccga
     3361 cggctcgttc agctacaacc cggcccccgg cttcaccggc accgactcgt tcacctacaa
     3421 gtccaacgac ggcagcctcg atagcaacgt ggccacggtg acgatccgcg tcgccccggc
     3481 cggcgacacg gaaggcaagg tgac
//
LOCUS       DBNGOH010000137         6047 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2402214, whole genome shotgun sequence.
ACCESSION   DBNGOH010000137 DBNGOH010000000
VERSION     DBNGOH010000137.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6047)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6047)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6047
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2402214"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(50..1018)
                     /locus_tag="AC159P_02565"
     CDS             complement(50..1018)
                     /locus_tag="AC159P_02565"
                     /inference="COORDINATES: protein motif:HMM:NF024799.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR3694365.1"
                     /translation="MKFRLCALLLIVIAMSVLVASACQRAGGGGGNKRVIAVIPKGVA
                     HFFWQSVHAGAEAAAKESNVEVVWKGPAQETDYSGQINIVEDAINRRVDGIVLAPTHR
                     DSLVPVVERAQRDGIPVAIFDSGIGTDKYVSYVATDNRQGGAQAAERLASKLGGKGRV
                     AILGVKAGSVSTDEREQGFQETIKQKYPGIEIVAFQYGESDRARSLDRAADILTAHPD
                     LDAFFASNESSTVGAVQAIKQKGLAGKVVLVGFDSSPNLVEDLKAGAIDSLVLQNPYK
                     MGYEAVKAMVAKLDGQSPPRQVDTGVKLLKKDNLETPEMQQLLKQP"
     gene            complement(1042..2022)
                     /locus_tag="AC159P_02570"
     CDS             complement(1042..2022)
                     /locus_tag="AC159P_02570"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013565696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR3694366.1"
                     /translation="MSGQTFLLTGAHGFIGAWVVKRLLAAGARVVIFDKSTDARRLRL
                     IMGEEEVARARVVTGDITEADALPPVVEEFAVTHVIHLAGLQVPTCRADPRLGALVNV
                     VGTINVFEAARLSAGRIRKIVYASSAAVFGATDEDRPVTERDAPEPASHYGVFKRCNE
                     GNARIYHLEHGLSSVGLRPLTVYGVGRDFGVTSDPTKAMKAAVVGRPFRIRFGGRTDF
                     QYVADTADTFIRAAVSDLEGAHVFNLHGETVRIEDVVAEIESAWPAARGLITHEEAPL
                     AIPAELDDAAIRAALGELPRTPLSEGVRATIGRFAELRREGRLETSDLDQ"
     gene            complement(2190..3356)
                     /locus_tag="AC159P_02575"
     CDS             complement(2190..3356)
                     /locus_tag="AC159P_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HIR3694367.1"
                     /translation="MSHELQVAFVSGPAYDPLYECLPEFIKTGGVGVNVAFSGDHPSL
                     NRHLAALGEVPYDLVSTHTKYAPSQLSFLAPLDGLVGASALGDFVPLLLELARVEGLL
                     YGLPRNIDVRLLHYRTDLLPAPPATWDELLETARRHNSPPDLYGFLFPGRESGLFGTF
                     FELAEMAGARLFPDTLVPEIENEGGRWALRLLRTFYAEGLVPPELPEWHYDKVHEAFR
                     AGRAAMVGDWPGYYGLYRDARVSLVRDRFALSPYPCGPAAKSLAYGGGHTFALTRRGA
                     EKPEALRLLLHLTAREQQLFEARRGSVPVRRSVMREVQAEADGANRARLSMLEEVVAA
                     HILIPPKFARYPEVEEVLWRTVQRAVVGAMEIDDALAHMRSQISEIVQAELQNV"
     gene            complement(3458..4756)
                     /locus_tag="AC159P_02580"
     CDS             complement(3458..4756)
                     /locus_tag="AC159P_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010586921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuconate dehydratase"
                     /protein_id="HIR3694368.1"
                     /translation="MSITITEIEALDIRFPTSRELDGSDAMNADPDYSAAYVILHTDA
                     GLEGHGLTFTIGRGNDICRAAVEAIAAPLKGRTLGSLTADMGAFWDELTGDSQMRWLG
                     PEKGVVHLATAAVVNAVWDLYAKAEGAPLWKLLVDMTPEQTVACVPFRYITDALTREE
                     ALELLRRQEATRGEREAEMREHGYPAYTTSAGWLGYSDEKIRRVVREALRAGWTHFKM
                     KVGRSLEEDVRRAGLIREEIGWQRTLMVDANQVWDVPRAIEWMKELAPFKPLWIEEPT
                     SPDDVLGHAAIARAVAPVGVATGEHCQNRIMFKQFLQAGALSFCQIDACRLGGVNEVL
                     AVLLMAAKFGVPVCPHAGGVGLCEYVQHLSIFDYVAVSGTLEGRRAEFVDHLHEHFVH
                     PVVIRDARYAAPLAPGYSIEMKPDSLRRYAFPRGEAWATA"
     gene            complement(4838..6025)
                     /locus_tag="AC159P_02585"
     CDS             complement(4838..6025)
                     /locus_tag="AC159P_02585"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020599259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HIR3694369.1"
                     /translation="MMKTRMIRLLTFCASVVLACPPVAGQRAAFDVKAFERARVLKAA
                     KQYLSESPATVTASSSPRSAGGRHDFFSEGDYWWPDPQNPAGPYVQRDGMTNPDNFTE
                     HRRYLMRLSVQVPALAAAWELTKDGRYAVHAARHLRAWFLDPATRMNPHLEYAQAIKG
                     RATGRGIGIIDTIHLVEVARAVEVLEGSRALSPSDSKGVKGWFADYLEWMTTHPYGID
                     EREAKNNHGTCWVMQVAAFARLTGNKELLDYCRQRFKTVLVPNQMAADGSFPQEMRRT
                     KPYGYALFNLEALAAAAEILSTPEDDLRRFRLADGRGLSRAVEFMAPYVRDKKSWPLK
                     PDVMYHEHWPMRQTSLLFAGIAFERPDYLALWKTLPADSAVEEVVRNFFIRQPVLWVK
                     NKY"
BASE COUNT          946 a         2047 c         2007 g         1047 t
ORIGIN      
        1 agtgatgcgg agcctactgc ttactgccta ctgcctactg cctactgttt catggttgtt
       61 tcaacagttg ctgcatctcg ggtgtttcga ggttgtcttt cttcaggagc ttgacgccgg
      121 tgtccacctg tctgggcggc gactggccgt cgagcttggc gaccatcgct ttgacggctt
      181 cgtagcccat cttgtagggg ttctggagga caagcgaatc gatggccccg gccttgaggt
      241 cttcgacgag gttcgggctg gagtcgaagc cgacgaggac gaccttgccc gcgagaccct
      301 tctgcttgat ggcctgcacg gcgccgacgg tcgaggactc gttcgaggcg aagaaggcgt
      361 cgaggtcggg gtgcgccgtc aggatgtccg ccgcgcggtc gagcgagcgg gcgcggtcgc
      421 tctcgccata ctggaaggcc acgatctcga tgccgggata cttctgcttg atcgtctcct
      481 gaaagccctg ctcgcgctcg tcggtcgaga ccgacccggc cttgacgccg aggatggcga
      541 ccctcccttt acctcccaac ttcgacgcca gccgctcggc ggcctgcgcg ccgccctgac
      601 ggttgtcggt ggcgacgtag gagacgtact tgtccgtgcc gatgcccgaa tcgaagatgg
      661 cgacggggat gccgtcgcgc tgcgcgcgct cgacgacggg gacgagcgag tccctgtgcg
      721 tgggcgcgag cacgatgccg tccacgcgcc ggttgatggc gtcctcgacg atgttgatct
      781 ggcccgaata gtccgtctcc tgcgccgggc ctttccacac cacctcgacg ttcgactcct
      841 tcgccgccgc ctccgcgccc gcgtgcacgc tctgccagaa gaagtgcgcg acgcccttcg
      901 ggatgaccgc gatgacgcgc ttgttgccgc cgccgccgcc cgcgcgctga cacgccgacg
      961 cgacgagcac ggacatggcg atgacgatca agagcaacgc gcagagtctg aacttcatgg
     1021 tttcgctccc aagctaaaaa ctcactggtc gaggtcgctc gtctcaagac ggccttcgcg
     1081 ccgaagctcc gcgaagcgcc cgattgtggc gcgcacgcct tccgagagcg gcgtcctcgg
     1141 caactctccg agcgccgcgc ggatggccgc gtcgtcaagc tccgcgggga tggcgagcgg
     1201 cgcctcttcg tgcgtaatca gcccgcgcgc cgcgggccac gcgctctcta tctcggcgac
     1261 gacatcctcg atgcgcaccg tctcgccgtg caggttgaag acgtgcgcgc cttcgaggtc
     1321 ggagacggcg gcgcggatga aggtgtcggc ggtgtcggcg acgtactgaa agtccgtgcg
     1381 cccgccgaag cggatgcgga agggacgccc gaccaccgcc gccttcatcg ctttggtcgg
     1441 gtcggacgtg acgccgaagt cgcggcccac gccgtagacc gtgagcggcc tgaggccgac
     1501 gctcgacagc ccgtgttcga gatgatagat gcgcgcgttg ccctcgttgc agcgcttgaa
     1561 gacgccgtag tggctcgcag gctccggcgc gtcgcgctcg gtcacgggcc gatcttcgtc
     1621 ggtcgccccg aagacggcgg cggagctggc gtacacgatc ttcctgattc ggcccgcgga
     1681 caggcgcgcc gcctcgaaga cgttgatcgt gccgacgacg ttgaccagcg cgccgagccg
     1741 cgggtctgcg cggcaggtcg gcacctgaag ccctgcgagg tggatgacgt gcgtgaccgc
     1801 gaactcttcg acgacgggcg ggagggcgtc ggcttcggtg atgtcgcccg tcaccacgcg
     1861 ggcgcgcgcg acctcctctt cgcccatgat gagacgcagg cggcgcgcgt ccgtgctctt
     1921 gtcgaagatg acgacgcgcg ccccggcggc gagcagccgc ttgacgaccc acgcgccgat
     1981 gaagccgtgc gcgccggtga gcaggaacgt ttgcccgctc atctgctcat cattaccttc
     2041 tcttcgctca gtcatcatca acgaaactct gccgacgaat catttgtagg ggcagggcaa
     2101 gtccgtggcc cgttgcgctg cgatcgattc gatctcgaat tcaatgtgcg ttgagcgggc
     2161 cgaggacaag ccctgcccct acgaaattct cacacgttct gaagctccgc ctgcacgatt
     2221 tcgcttatct gacttcgcat gtgcgcgagc gcgtcgtcta tctccatcgc tccgaccacg
     2281 gcgcgctgca cggtgcgcca cagcacctct tcgacctcgg ggtagcgcgc gaacttcggc
     2341 gggatcagga tgtgcgcggc gaccacctct tcgagcatcg agaggcgcgc gcggttcgct
     2401 ccgtcggcct ccgcctgcac ctcgcgcatg acggagcggc gcacggggac gctgcccctg
     2461 cgtgcctcga acagttgttg ctcgcgggcc gtcaggtgga gcagcagccg cagcgcctcg
     2521 ggcttctcgg ccccgcgccg cgtcagcgcg aacgtgtggc cgccgccgta ggcgagcgac
     2581 ttcgcggcgg ggccgcaagg gtacggactc aaagcgaagc ggtcgcggac gagcgacacg
     2641 cgcgcgtcgc ggtagaggcc gtagtaaccg ggccagtcgc cgaccatcgc cgcgcggccc
     2701 gcgcggaaag cctcgtgcac cttgtcgtag tgccactcgg gcagctccgg aggcaccagt
     2761 ccttcggcgt agaacgttct caacagtcgc agcgcccagc gcccgccctc gttctcgatc
     2821 tccggcacga gcgtgtcggg gaagagccgc gcgcccgcca tctcggccag ctcgaagaag
     2881 gtgccgaaca gtcccgactc gcggcccgga aagaggaagc cgtaaaggtc gggcggcgag
     2941 ttgtgacgcc tcgccgtctc aagcagctcg tcccacgtcg cgggcggcgc aggcaaaagg
     3001 tctgtgcggt agtgcagcag ccgcacgtcg atgttgcgcg gcagcccgta gagcaagccc
     3061 tcgacgcgcg ccagctcaag cagcagcggg acgaagtctc cgagcgccga cgcgccgacc
     3121 aatccgtcca gcggcgcgag aaagcttaac tgcgaggggg cgtacttcgt gtgcgtcgag
     3181 acgagatcgt agggcacttc gccgagcgcg gcaaggtgtc gattcagcga cgggtggtcg
     3241 ccgctgaacg cgacgttgac cccgaccccg cccgtcttta tgaactccgg caggcactcg
     3301 tacagcgggt cgtacgccgg gccgctgacg aaggcgactt gcagttcgtg actcatctct
     3361 ggctcctcac gcaaggggaa aaggggaagg gggaaagggc gaaaaggaag aagcattctt
     3421 tcccttttcg cctcttcccc ttttgacctt ttcgccctca tgccgtcgcc cacgcctcgc
     3481 cgcgcgggaa cgcgtagcgc ctgagcgagt cgggtttcat ctcgatgctg tagccgggcg
     3541 cgagaggcgc cgcgtagcgc gcgtcgcgga tgacgaccgg gtggacgaag tgctcgtgga
     3601 ggtgatcgac gaactccgcg cggcggcctt cgagcgtgcc gctgacggcg acgtagtcga
     3661 agatcgagag gtgctggacg tactcgcaca ggcccacccc gcccgcgtgc gggcagaccg
     3721 gcacgccgaa cttcgcggcc atcaagagca cggccagcac ttcgttgacg ccccccagac
     3781 ggcacgcgtc gatctggcag aaggagagcg cgccggcctg caagaactgc ttgaacatga
     3841 tgcggttctg gcagtgctcg ccggtcgcca cgcctaccgg cgcgacggcg cgcgcgattg
     3901 ccgcgtggcc gagcacgtcg tccggcgagg tcggctcttc gatccacagc ggcttgaacg
     3961 gcgccagctc cttcatccac tcgatcgcgc gcggcacgtc ccacacctga ttggcatcca
     4021 ccatcagcgt ccgctgccag ccgatctcct cgcggattaa gcctgcgcgc cgcacgtctt
     4081 cttcgaggct ccggcccacc ttcatcttga agtgcgtcca ccccgctcga agcgcctcgc
     4141 ggacgacgcg ccgtatcttc tcgtccgagt agccaagcca gccggcggag gtcgtgtaag
     4201 ccggatagcc gtgctcgcgc atctcggcct cgcgctcgcc tcgcgtcgcc tcctgccgac
     4261 gcagaagctc cagcgcctct tcgcgcgtca gcgcgtccgt gatgtagcgg aacggcacgc
     4321 acgcgaccgt ctgctccggt gtcatgtcaa cgagcagctt ccagagcggc gcgccttcgg
     4381 ccttcgcgta caagtcccac acggcgttga cgaccgccgc ggtcgccaga tggacgacgc
     4441 ccttctcggg gccgagccag cgcatctggc tgtcgcccgt caactcgtcc cagaaggcgc
     4501 ccatgtccgc ggtcagagac ccgagcgtgc ggcccttgag cggcgcggcg atggcctcga
     4561 ccgccgcgcg gcagatgtcg ttgccgcgcc cgatggtgaa cgtcagcccg tgcccttcca
     4621 gccccgcgtc ggtgtgcagg atgacgtacg ccgccgagta gtccgggtcg gcgttcatcg
     4681 cgtccgaccc gtccagctcg cgcgaggtcg ggaagcggat gtcgagcgct tcgatctccg
     4741 taatcgtaat agacatgatt tcaatagtga tgagtactga gtgctgagta ctgagtgaaa
     4801 acaagctgct ttcttctcag tactcagtac tcagtcctca gtacttattt ttcacccaca
     4861 gcacgggctg gcggatgaag aaattgcgga cgacctcttc gaccgcggag tccgcgggga
     4921 gcgtcttcca gagcgcgagg tagtcgggcc gctcgaacgc tatccctgcg aagaggaggc
     4981 tcgtctgccg catcggccag tgctcgtggt acatcacgtc gggcttgagc ggccagctct
     5041 ttttgtcgcg gacgtagggc gccatgaatt cgacggcgcg gctgagtccg cgcccgtccg
     5101 cgagccggaa ccgccgcaga tcgtcctccg gcgtcgagag tatctcggcg gcggcggcca
     5161 gcgcttcgag gttgaagagc gcgtagccgt acggcttcgt gcgccgcatc tcctgcggga
     5221 agctgccgtc cgccgccatc tggttcggca cgagcaccgt cttgaagcgc tggcggcagt
     5281 agtcgagcag ttctttgttt cccgtcagcc gcgcgaacgc ggcgacctgc atcacccagc
     5341 acgtgccgtg gttgttcttc gcctcgcgct cgtcgatgcc gtacgggtgc gtcgtcatcc
     5401 actcgagata atccgcgaac caccccttga cgcccttcga gtcggacggc gacagcgcgc
     5461 gcgagccttc gagcacttcg acggcgcgcg cgacctcgac gaggtggatg gtgtcgatga
     5521 tgccgatgcc gcgcccggtc gcgcgcccct tgatggcctg cgcgtattcg aggtgcgggt
     5581 tcatccgcgt cgcggggtct aagaaccacg cgcgcaggtg gcgcgcggcg tggacggcgt
     5641 agcgcccgtc cttcgtcaac tcccacgcgg cggcgagcgc gggcacctgc acgctgaggc
     5701 gcatcaggta gcgacgatgc tccgtgaagt tgtcggggtt cgtcatgccg tcgcgctgca
     5761 cgtacggccc cgcggggttc tgcgggtcgg gccaccagta gtcgccctcg gagaagaagt
     5821 cgtgtctgcc cccggcgctg cgcgggctgg aagaggccgt gacggtggcg ggcgactcgg
     5881 agaggtactg cttcgccgcc ttcagcacgc gcgcccgctc gaacgccttc acgtcgaaag
     5941 ccgcgcgctg ccccgcgacc ggcgggcacg ccagcacgac cgacgcgcag aatgtgagca
     6001 gccgaatcat tcgagtcttc atcatccgag tcttacttga acgacgc
//
LOCUS       DBNGOH010000138         2830 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2402315, whole genome shotgun sequence.
ACCESSION   DBNGOH010000138 DBNGOH010000000
VERSION     DBNGOH010000138.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2830)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2830)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2830
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2402315"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2617
                     /locus_tag="AC159P_02590"
     CDS             <1..2617
                     /locus_tag="AC159P_02590"
                     /inference="COORDINATES: protein motif:HMM:NF024180.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HIR3694370.1"
                     /translation="VSGLASCFSPGGQGGGDQPGEPPPMGRAPDRPDGVGRADVRVFD
                     ADGNPVKDAKAELSSKRPNGFSCDSGQSTDAAGRALLPPLHVGKLKLSIAANGFQTLE
                     AAIEPGRLAEPVRVVLSPKGAQAASRLAQAKGGTPSPARCFDLYRLLDSYLTGELGRA
                     RANYLGGRFDDANKHFVNLLAAAGDDSPAGKLARASRFRAAARTGLGDVAYSKGDYAA
                     ALRLYTEAVGGASKDRRHELMWAAQRGIGRTLWTLAGRENSPPEAARLRAESLKAYED
                     SIKTVETLLAGSLRSDEARTSFLALTKDVFDEASGAYAELALAATPPSSPVLDGPALT
                     KAAAAFRIAEQGRARSLLELLSETGAELTEGIPAELLRRRSDIQARRYEIVELLNGVD
                     AEGVTPGRPAQALESELRELELARIGVENQIRTSSPRYEALTKPQPLSVEEVQQNVLD
                     ADTVLLEYSLGEERSYLWAVTGTGVRLFRLPARSEVETLVAEFRAKLIPRDLRRPITE
                     TDANGATREQGRGLVLAHEETPQRVGAYAAAAHALYKAVVEPASSLVGGKRLLVVADG
                     ALHYAPFGALVTSGEGSTYDALAYLVKTNEVVYAPSASVVAAVRRQAAGGATGNGVLL
                     IADPVFDAGDPRASRTPANPQGRTGSARPLRLDSALEDVTRTPPGGLRLARLPGSRTE
                     AEQVAKLARASGRWPDELLDSSASKTNLLSRDLTRYCILHFATHGLLNAERPQFTGLA
                     LSPVWDSEDDGFLKVDEIFNLRLGSPLVMLSACETGLGKQSRGEGVVGLARAFMHAGA
                     PTVGVSLWSVADLSTAELMPEFYKHLLTEEPASPSSALRAAQLTLIEGKRFSAPFHWA
                     AFVLVGDWRRRSLST"
BASE COUNT          456 a          966 c          958 g          450 t
ORIGIN      
        1 cgtgtccggt ctcgcgtcct gcttctcgcc gggcggtcag ggcgggggcg accagcccgg
       61 agagccgccc ccgatgggcc gtgcccccga ccggcctgac ggcgtcgggc gcgccgacgt
      121 gcgggtcttc gacgcggacg ggaaccccgt taaagacgcg aaagccgagc tgagttccaa
      181 gcgcccgaac gggttctcct gcgacagcgg gcagagcacc gacgccgcgg gccgcgccct
      241 cctgcccccc ctgcacgtcg gtaaattgaa gctcagcata gcggctaacg ggtttcagac
      301 gctggaggct gccatcgaac cgggacggct ggcggaacct gtgcgcgtcg tgctctcccc
      361 caaaggcgcg caggcggcca gccgcctcgc gcaagccaag ggcggcaccc cttcacccgc
      421 ccgctgcttc gacctttacc gtctgctcga cagttatctc acgggcgaac tcgggcgggc
      481 gcgcgcgaat tatctgggcg ggcggttcga cgacgcgaac aagcatttcg tcaacctgct
      541 cgccgccgcg ggcgacgatt cgcccgcggg taaactcgcg cgggcgagca ggtttcgtgc
      601 cgccgcgcgc accggcctcg gcgatgtcgc atatagcaag ggtgattacg cggcggcgct
      661 gcgcctctac acggaagcgg tcggcggcgc gagcaaggat cggcggcacg aactgatgtg
      721 ggccgcgcaa cgcggcatcg gcaggacgtt gtggactttg gccgggcggg agaacagccc
      781 gcccgaagcg gcccggctcc gcgcggaatc tctgaaggct tacgaggact cgatcaagac
      841 ggtcgagacg ctcctcgcag ggtcgctgcg ttcggacgag gcccgcacgt ctttcctcgc
      901 gttaactaaa gatgtcttcg acgaggcgtc gggcgcctac gccgagctgg ccctggcggc
      961 gacgccgccg tcctcgccgg tgcttgacgg ccctgccttg acgaaggccg ccgccgcctt
     1021 ccggatagct gagcaggggc gcgcgcgctc tctgctcgaa ctcctgagtg agaccggggc
     1081 ggaactcacg gagggaattc ccgcggaact gctcaggcgc agatccgaca tacaggcccg
     1141 ccggtacgag atcgtagaac tgctgaacgg cgttgacgcg gagggggtga cgcccggacg
     1201 ccccgcgcaa gcccttgagt cggaactcag agagttggaa ctggcgcgca tcggcgtcga
     1261 gaaccagatc aggacttcca gcccccgtta cgaggcgctc accaagccgc aaccgctgtc
     1321 cgtcgaggag gtgcagcaga acgtgctcga cgccgacacc gtgctgttgg agtacagcct
     1381 gggcgaagag cgctcgtacc tctgggccgt gaccggcacc ggggttcgac ttttcaggct
     1441 gcctgcccgc tccgaggtcg agacgctggt ggcggagttt cgcgcgaaac tgattccgcg
     1501 cgacctccgc cgcccgatca cggagacgga cgcgaacggc gcgacccgcg agcaggggcg
     1561 cggccttgtg ctcgcgcatg aagagacgcc gcagcgcgtg ggcgcctacg ccgcggcggc
     1621 ccacgcactt tacaaggcgg tggtcgaacc tgcgtcgtcg ctcgtcggcg gcaagcgcct
     1681 cctcgtcgtc gccgacgggg cgctccacta tgcgcccttc ggcgcgctcg tcacctccgg
     1741 cgaaggctcc acttacgacg ccctagccta cctggtgaag accaacgaag tcgtctacgc
     1801 gccgtcggcc tccgtcgtcg ccgccgtgag gcggcaggcc gcgggcggcg ccaccggcaa
     1861 cggggtgctg ctgatcgccg acccggtctt cgacgccggc gacccgcgcg cgagccggac
     1921 gccggccaac ccgcagggtc ggacaggctc ggcgcgtccc ctccgactcg acagcgcgct
     1981 cgaagacgtg acccgaacgc ctccgggcgg actgagactc gcgcggttgc ccggctcccg
     2041 caccgaggcg gaacaagtgg cgaagctggc gcgcgcgtcc gggaggtggc ccgacgaact
     2101 gctcgactcc tccgccagca agaccaattt attgagccgg gacctgacgc ggtactgcat
     2161 cctgcacttc gccacgcacg ggctgctcaa cgccgagcgc ccgcagttca cgggactggc
     2221 cctctcgccg gtctgggaca gcgaggacga cggcttcctg aaggttgacg agattttcaa
     2281 cctccggctg gggtcgccgc tggtcatgct ctcggcctgc gaaacggggc ttgggaagca
     2341 gagccggggc gaaggcgtcg tcggcctcgc acgcgccttc atgcacgcgg gtgcgccgac
     2401 cgtaggggtc agcctatggt cggtggccga cctgtcaacg gcagaactga tgccggagtt
     2461 ctataaacac ttgctgaccg aagagccggc gtcgccctcc tccgcgctcc gcgccgccca
     2521 actgaccctg atcgagggca agcgcttcag cgcccctttc cactgggccg ccttcgtgct
     2581 cgtcggcgac tggcgccgga ggagcctgag cacataagcg ggttccaata caacccgcct
     2641 ttcggcctca gtggaccggg tgatgtccgg ccttcggttg cttctttctg gtcacgaagg
     2701 tcgaatgggt ggtattcata caatgaaggt cgaggtggat atggagtgaa agttcgatga
     2761 agccctaccg attcattttc cattcgattg tcatcgtagc ttgttgtagc tttgtctgcg
     2821 cacactcttg
//
LOCUS       DBNGOH010000139         1801 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3502423, whole genome shotgun sequence.
ACCESSION   DBNGOH010000139 DBNGOH010000000
VERSION     DBNGOH010000139.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1801)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1801)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1801
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3502423"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..430)
                     /locus_tag="AC159P_02595"
     CDS             complement(<1..430)
                     /locus_tag="AC159P_02595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694371.1"
                     /translation="MPVPTAEQGLPKAAHVPPRRRAGGHPRMPSTVGYMVAAGGLSVA
                     LFLVIWFMLRADGDEAPWIPAGLAAGFVILVAAAAREVVMRRAWSRYTRELEPDMRHG
                     TVARAVIKPAAGARANAQSSVAAMRALQQRIAEMDGANVPP"
     gene            759..1130
                     /locus_tag="AC159P_02600"
     CDS             759..1130
                     /locus_tag="AC159P_02600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694372.1"
                     /translation="MATTCKKCGATATGDQAFCQKCGAVIGMEGAAPNPDAPPSLAAT
                     VVGQRFNIPTPPPKPKPTPPAPPPPKPTPAPASAQGAGGAADGKSGSKLVLLVVAGFI
                     AVMLIGGLLLVLLYLFFGGHI"
     gene            1173..>1801
                     /locus_tag="AC159P_02605"
     CDS             1173..>1801
                     /locus_tag="AC159P_02605"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024760.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HIR3694373.1"
                     /translation="MEQFDVSPYPPRAAAAPYLPPQRRGGLAVAAWALAALVALLLGL
                     ITLLIIGFGTGPVPFLIGLCVAVLPVPVYVTLVLWIDRYEAEPLWMLATAFFWGALVA
                     TFFAIVVNSLGAAFVEEAFDAEAASFFGTVVSAPLVEECAKAFALFALFFWKRDEFDG
                     VLDGIVYAAMVGLGFAMTENIKYYGEAVLAGNAAGVFVVRGLFSPFAHPL"
BASE COUNT          237 a          653 c          607 g          304 t
ORIGIN      
        1 ccggcgggac gttcgcgccg tccatctcgg cgatgcgctg ttggagcgcg cgcatcgcgg
       61 cgacggagct ttgcgcgttg gcgcgcgcgc ccgccgccgg cttgatgacg gcgcgcgcca
      121 ccgtgccgtg tcgcatgtca ggttccagct cgcgcgtgta gcgcgaccac gcgcgacgca
      181 tgacgacctc gcgcgcggcg gcggcgacga ggatgacgaa gcccgccgcc agtcccgccg
      241 gtatccacgg cgcctcgtcg ccgtcggcgc gcagcatgaa ccagatcacc agaaagagcg
      301 cgaccgagag gccgccggcc gcgaccatgt agccgacggt cgaaggcatg cgcgggtgcc
      361 cgcccgcgcg gcggcgcggc gggacgtgcg ccgccttcgg caatccttgc tcggctgtgg
      421 gcactggcaa tagtgtgtct ccgggtggaa gcgaaacgcg cgcgcggcgg acgcggcacg
      481 tgagcgccgc gccgccgctg atgagacgtg gattatactc aatcttttcc gccgcgtctg
      541 tcaattcacg cgcgagtgcc gcgcccgtca ctttcgtgct ggaagtcggg tgtggttgtg
      601 tttatactta ccggcgtttt acggcccgct tcgacccggg ccgacagccg tacattcatc
      661 cgtccacgga gccggttccc ggcgaagggc gagccgacga gcgttcgagg gggcgtcgca
      721 cctggggcat ccgtcacaag ggttgactac ataattcgat ggctacgacc tgcaagaagt
      781 gcggcgcgac ggcgaccggc gatcaggctt tctgccagaa gtgcggcgcc gtcatcggca
      841 tggagggcgc ggcgccgaac ccggacgcgc cgcccagcct cgccgcgacc gtcgtcggcc
      901 agcgcttcaa catcccgacg ccgccgccga agccgaagcc gacacctccg gcaccgccgc
      961 cgccgaagcc gacgccagca cccgcttcgg ctcaaggcgc gggcggcgcg gctgacggaa
     1021 agtcgggcag caaactcgtg ctgctcgtcg tcgcgggctt catcgccgtc atgctgatcg
     1081 gcggcctcct gctcgtgctg ctctacctct tcttcggcgg ccacatttga gggacgattc
     1141 accgagacgc ccccgccggt gaacccagac ttatggaaca gttcgacgtt tccccctacc
     1201 cgccgcgcgc agccgcggcg ccctacctgc cgccgcagag gcgcggcggg ctggcggtgg
     1261 ccgcgtgggc gctggccgcg ctggtcgcgt tgctgctcgg cctcatcacg ctcctcatca
     1321 tcggcttcgg caccggcccc gtgccgttcc tcatcggcct ctgcgtcgcc gtgctgcccg
     1381 tccccgtcta cgtgacgctc gtgctgtgga tcgaccgcta cgaagccgag ccgctctgga
     1441 tgctcgccac cgccttcttc tggggcgcgc tggtcgccac cttcttcgcc atcgtcgtca
     1501 actcgctcgg cgccgcgttc gtcgaggagg cgttcgacgc ggaggccgcc tccttcttcg
     1561 gcacggtcgt ctcggccccg ctcgtcgagg agtgcgcgaa ggcgttcgcc ctcttcgcgc
     1621 tcttcttctg gaagcgggac gagttcgacg gcgtgctcga cggcatcgtc tacgcggcga
     1681 tggtcggcct cggcttcgcc atgaccgaga acatcaagta ctacggcgag gccgtgctcg
     1741 caggcaacgc cgcgggcgtc ttcgtcgtgc gcgggctgtt ctcgcccttc gcgcacccgc
     1801 t
//
LOCUS       DBNGOH010000140         2662 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2769688, whole genome shotgun sequence.
ACCESSION   DBNGOH010000140 DBNGOH010000000
VERSION     DBNGOH010000140.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2662)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2662)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2662
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2769688"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1599)
                     /locus_tag="AC159P_02610"
     CDS             complement(<1..1599)
                     /locus_tag="AC159P_02610"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HIR3694374.1"
                     /translation="MPDKKQAPAKDNRLLVKLRPSGALRAAEARTNLQPLYDAASPSA
                     TAAFGLDAAAPQWFLAELPDGAAGPWDLAHARVAAQLGVSESDVVFAEPDLVHDVYRD
                     TNEAEVGAVLAAADQCVADPQDGTRNKALGPDAFAWHLDNDHSQLGAARDAVEFKPPR
                     TRIAHLDTGYYRAHETVPEHVLRPLERSFVEGDADRHSAEDPDNRVFLIDNSGHGTGT
                     LGILAGGKSASFGNVYLGGAPGAEVLPLRVSDRVVLLRTSALARALDYAVEQRCDVAT
                     LSMGGTPSQAWAEAVDKAYMAGLCLCAAAGNHIGFTPPQTLVYPARYSRVIAVCGVMA
                     DGRPYADLKGTALEGSFGPDSAMKAAIAAYTPNIPWPRFMCGTVIRLNGEGTSAATPQ
                     VAAAAALWFEKYKHELPRDWRRVEAVRNALFASAKMKTDRKHFGNGVLQARAALNVRP
                     VFPRDPSPKSDNSFSLFRVITGLGVVEPPPREQMFNLELSQRWMLNPELQEIVPDPAA
                     SARLDDKTLQRFMEALIEDEGASLA"
     gene            complement(1774..>2662)
                     /locus_tag="AC159P_02615"
     CDS             complement(1774..>2662)
                     /locus_tag="AC159P_02615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3694375.1"
                     /translation="EALEAAERGLYFEPEHVGCINLRAMALNHLGLEDEAAVAIEGAL
                     GVEPENALTHANRGWQEVHRGDYERALTHFREALRIDAELEWAREGVVEVLKARNPVY
                     RVMLRYFLWSSRLSGRATWLFIIGFYVLSRLIRAGIRAQPELAPILWPLYGLYLAFVL
                     MTWIARPLFDLLLRLDPIGRVVLSKKQIAASNWVGAAIVLGLSGLVLGLLTGVTSFYL
                     LAAGAGMMTMPIAGAFAADTPRARRVLPLYAVGLGALALVAFALSFYDFDAAMVFGTI
                     FFLGFFVYGWVANALTIRT"
BASE COUNT          408 a          856 c         1005 g          393 t
ORIGIN      
        1 ggcgagcgag gcgccttcgt cttcgatgag cgcttccatg aagcgttgca gcgtcttgtc
       61 gtcgagccgg gccgaggcgg cggggtcggg gacgatctct tgcagctccg ggttgagcat
      121 ccagcgctgc gagagttcga ggttgaacat ctgctcgcgc gggggcggct cgacgacgcc
      181 gaggccggtg atgacgcgga agagcgagaa cgagttgtcg gacttgggac tcgggtcgcg
      241 cgggaagacg ggcctgacgt tgagcgccgc gcgcgcctga agcacgccgt tgccgaagtg
      301 cttccggtcg gtcttcatct tggccgaggc gaaaagcgcg ttgcgcaccg cctcgacgcg
      361 gcgccagtcg cgcggcagct cgtgcttgta cttctcgaac cagagcgcgg cggcggcggc
      421 cacctgtggc gtggcggcgg atgtcccctc gccgttgagg cggatcaccg tgccgcacat
      481 gaaccgcggc cacgggatgt tcggcgtgta ggcggcgatg gccgccttca tcgctgagtc
      541 ggggccgaag ctgccttcga gcgcggtgcc cttgaggtcg gcgtaggggc gcccgtcggc
      601 catcacgccg cagacggcga tgacgcggct gtagcgcgcg gggtagacga gcgtctgcgg
      661 cggcgtgaag ccgatgtggt tgcccgccgc ggcgcagagg cagaggcccg ccatgtacgc
      721 cttgtccaca gcctcggccc aagcctgcga gggcgtgccg cccatgctca gcgtggccac
      781 gtcgcagcgc tgctcgacgg cgtagtcgag cgcgcgcgcg agcgcgctcg tacgcagcag
      841 cacgacgcgg tcggagacgc gcagcggcaa cacctccgcg ccgggcgcac cgccgagata
      901 gacgttgccg aaggacgcgg acttgccccc ggcgaggatg ccgagggtgc ctgtgccgtg
      961 gccggaattg tcgatgagga agacgcggtt gtcggggtct tcggcgctgt gccggtcggc
     1021 atcgccttcg acgaagctgc gttcgagggg gcgcaggacg tgctcgggga cggtctcgtg
     1081 cgcgcggtag tagcctgtgt cgaggtgcgc gatgcgcgtg cgcggcggct tgaactcgac
     1141 ggcgtcgcgc gccgcgccga gctgcgagtg gtcgttgtcg aggtgccagg cgaaggcgtc
     1201 ggggccgagc gccttgttgc gcgtcccgtc ctgcgggtcg gccacgcact ggtcggcggc
     1261 ggcgagcacg gcgccgacct ccgcctcgtt cgtgtcccgg taaacgtcgt ggacgaggtc
     1321 aggctcggcg aagaccacgt cggactcgga gacgccgagc tgcgcggcga cgcgcgcgtg
     1381 cgccaagtcc cacgggccgg cggcgccgtc gggcagctcg gcgaggaacc actgcggcgc
     1441 ggcggcgtcg aggccgaagg cggcggtggc cgaaggcgag gccgcgtcgt acagcggttg
     1501 caggttcgtg cgcgcctccg ccgcgcgcag cgcgcccgac gggcgaagtt tgacgagcaa
     1561 gcggttatct ttcgcgggcg cctgtttttt gtccggcact gtcttcctcc tccagagcgg
     1621 ttctcgaatg agtgagagac gttcggcaat actgtagggg cacggcttgt ccctgcccgc
     1681 gttgcgcttt cattcaacgt cgaatcagtg gaacgcgacg ggccgcggac aagcccagcc
     1741 cctacatgta ctacgctcgt tgtacggcgc gcttcaggtt ctgatggtca gcgcgttggc
     1801 gacccagccg tagacgaaga agccgaggaa gaagatggtg ccgaagacca tcgccgcgtc
     1861 gaagtcgtag aaggagagcg cgaaggcgac gagcgcgagc gcgccgaggc cgacggcgta
     1921 gaggggtaag accctgcgcg cgcgcggcgt gtcggcggcg aaggcgccgg caatcggcat
     1981 tgtcatcatc cccgccccgg cggccagcag gtaaaaggaa gtgacgcccg tcaggaggcc
     2041 gagcacgaga cccgagaggc cgagcacgat cgcggcgccg acccagttcg acgccgcgat
     2101 ctgcttcttc gagagcacga cgcggcctat ggggtcgagc cggagcagga ggtcgaagag
     2161 cgggcgcgcg atccacgtca tcaggacgaa cgcgaggtag aggccgtaga gcggccagag
     2221 aatcggcgcc agctcgggct gcgcacggat gcccgcgcgg atgaggcgcg agaggacata
     2281 gaagccgatg atgaagagcc acgtcgcgcg ccccgacaga cgcgacgacc agaggaagta
     2341 gcgcagcatc acgcggtaga cggggttgcg cgccttcagc acttcgacca cgccctcgcg
     2401 cgcccattcg agttcggcgt cgatgcgcag cgcctcgcgg aagtgcgtca gcgcccgctc
     2461 gtagtcgccg cggtgcacct cctgccagcc gcggttggcg tgcgtgagcg cgttctccgg
     2521 ctcgacgccg agcgcgccct cgatggcgac cgccgcctcg tcctccagcc ccaggtggtt
     2581 gagcgccatc gcgcgcaggt tgatgcagcc gacgtgttcc ggctcgaaat acagcccccg
     2641 ctcggcggct tcgagcgcct cc
//
LOCUS       DBNGOH010000141         3549 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2586414, whole genome shotgun sequence.
ACCESSION   DBNGOH010000141 DBNGOH010000000
VERSION     DBNGOH010000141.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3549)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3549)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3549
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2586414"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1185)
                     /locus_tag="AC159P_02620"
     CDS             complement(<1..1185)
                     /locus_tag="AC159P_02620"
                     /inference="COORDINATES: protein motif:HMM:NF013799.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HIR3694376.1"
                     /translation="MNTEHKSFPRSVSSLLALLFIITTALAFNNAPRGATADSSSSLI
                     LHPSSFSSAKRAIVVSLDGLDVRYLREPDRYGLKIPTLRRLMKEGATARGGVLSVYPS
                     VTYPNHATMVTGALPTRHGIYANEVFDASAPQAGAWHWFARDLKADALWDAAGRGGLS
                     TALVSWPVSTGAGDWNVPEIWKAGVGPSALEATLAEMTAHQRPAGLLAEVARRDPALY
                     ARVTKDEGDDMRTRFAEYLIEQKRPGLVFVHLFDLDHFQHDFGPFTPEAHAALEKSDA
                     YLARLLAAAERSGTLAETAVFVVSDHGFRAYTTQIRPNVILARAGLIKVREQKNAEGR
                     ARSVLAEWRAFAVPSGGSCSVMLRDPNDKDALTRALAAFAEYTGTGRGAEAKKPAVLR
                     VLN"
     gene            complement(1383..>3549)
                     /gene="leuS"
                     /locus_tag="AC159P_02625"
     CDS             complement(1383..>3549)
                     /gene="leuS"
                     /locus_tag="AC159P_02625"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HIR3694377.1"
                     /translation="LSNEQSSGGVCWRCGAAVEKRDIEQWFLRITAYAEQLLADMEEI
                     EAGWPERVLTMQRNWIGRSEGAYVDFQIKDTDERVRVFTTRIDTIYGATAIVLAVDHP
                     LVELSPLKGDVRRFAERIRAARGAAARAPGEEEEKEGINTGLLAVNPFSGESIPVWAA
                     NYVLMEYGTGAVMSVPAHDDRDFEFAQKYSLPVRRVIERISHAQEAIPHDPEAEDQSV
                     AMPHAFTDYGITVNSGEWSGKLSADAMRVMAEHAKRKGFGDPAVTYRLRDWGVSRQRF
                     WGAPIPVVYCEKCGTVPVPEKDLPVTLPEHAEFTGAGRSPLAGVAEFVNATCPKCGGA
                     GARRETDTMDTFIDSSWYFFRYIDPRNEQAPFDKEVAAYWTPVDQYIGGVEHAVMHLL
                     YTRFWTKFMRDIGLVSFGEPVKNLMTQGMVVAETFYREGEDASQKNYFNPSEVETVRD
                     ERGRVREATLLSDGLPVTVGPFEKMSKSANNGVDPNDMMTAYGADATRLFILFAAPVE
                     NDLRWQEAGIDGALRFLRRVYSMVYRWRDALMDAPAPDAAEFSAAARKLRHETHRAIA
                     RVTDDFERLHFNTSVAALMELSNALGDFTPAPDAATASDLFAAREALEALVLMLAPFT
                     PHVAEEMWEGLGHEGGVLSDGARWPQADEALARKEELEIPVQVNGKVRGRVHVSADAT
                     EDELREAALADEKARAWTEGKQVVKIVVVPQRLVNIVVK"
BASE COUNT          541 a         1169 c         1257 g          582 t
ORIGIN      
        1 gttgaggacg cgcagcacgg cgggcttttt agcctccgcc ccgcggcccg tgccggtgta
       61 ttcggcgaag gcggcgagcg cgcgcgtgag tgcgtctttg tcgttggggt cgcggagcat
      121 gacggagcac gagccgccgg aaggaaccgc gaacgcgcgc cactcggcga ggacggagcg
      181 cgcccgccct tcggcgttct tctgctcgcg caccttgatg aggccggcgc gcgcgaggat
      241 gacgttcggt cgaatctgcg tcgtgtaggc gcggaagccg tggtcgctca cgacgaagac
      301 ggcggtctcg gcgagcgtgc cggagcgctc ggcggcggcg agcaggcgcg cgaggtaggc
      361 gtccgacttt tcgagcgcgg cgtgcgcctc cggggtgaag gggccgaagt cgtgctggaa
      421 gtggtcgagg tcgaagaggt gtacgaagac gaggccgggg cgcttctgct cgatgaggta
      481 ttcggcgaag cgcgtccgca tgtcgtcgcc ttcgtccttc gtcacgcgtg cgtagagcgc
      541 ggggtcgcgg cgcgcgacct ctgcgagcag cccggcgggc cgctggtgcg cggtcatctc
      601 ggcgagcgtc gcttcgagcg cggaggggcc gacgcccgcc ttccaaatct cgggcacgtt
      661 ccagtcgccc gcgccggtcg agaccggcca cgagacgagc gcggtcgaaa gcccgccgcg
      721 cccggcggcg tcccacagcg cgtcggcctt gaggtcgcgc gcgaaccagt gccacgcgcc
      781 cgcctgcggc gccgacgcgt cgaagacctc gttggcgtag atgccgtggc gtgtgggcag
      841 cgcgcccgtc accatcgtcg cgtggttcgg gtacgtcacc gaagggtaga ccgaaagcac
      901 gccgccgcgc gccgtcgcgc cctctttcat caaccgtcgc agcgtcggaa tcttcagccc
      961 gtacctgtcc ggctcgcgca ggtagcgcac gtccagcccg tcgagcgaga cgacgatggc
     1021 gcgcttcgcc gaagagaagg atgaaggatg aaggatgagg gatgaagaag aatcagccgt
     1081 cgcgccacgc ggcgcgttgt tgaaggcgag cgcagttgtg atgatgaaga gaagcgcgag
     1141 aagagaagag acggagcgcg ggaagctttt atgttcggtg ttcatcggcg tcgcggcatg
     1201 aagtgtcggt cgagcctggc gcacggctga tctaacacag agggcgcaga gacacagggg
     1261 gcacggagaa atcattcggg gccgagattc gacgtttcgg atttcgaatc tcgaatgccg
     1321 acgaaggcga aagggtgagg gatgaagaga gtgctgacag ccgctccttc atccctcgtt
     1381 cctcacttga cgacgatgtt gacgagccgt tgcgggacga cgacgatctt gacgacctgt
     1441 ttgccctccg tccacgcgcg cgccttctcg tcggcgagcg cggcctcgcg cagctcatcc
     1501 tccgtggcgt cggcgctgac gtggacgcgc ccacgcacct tgccgttgac ctgcacgggg
     1561 atttccagct cctccttgcg cgcgagcgct tcgtccgcct gcggccagcg cgcgccgtcc
     1621 gagaggacgc cgccctcgtg acccaagcct tcccacatct cttcggcgac gtggggcgtg
     1681 aagggcgcga gcatcaggac gagcgcctcc aaagcctcgc gcgcggcgaa gaggtctgaa
     1741 gccgtggccg cgtcgggcgc gggcgtgaag tcgccgagcg cgttcgacag ctccatcagc
     1801 gcggcgacgc tcgtgttgaa gtgcagccgc tcgaagtcgt ctgtaacgcg cgcgatggcg
     1861 cggtgcgttt cgtgccgcag cttgcgggcc gcggcggaga actcggcggc gtcgggcgcg
     1921 ggcgcgtcca tcagcgcgtc gcgccagcgg tagaccatcg agtagacgcg gcgcaggaag
     1981 cggagcgcgc cgtctatccc ggcctcctgc cagcgcaggt cgttctcgac gggcgcggcg
     2041 aagaggatga agagccttgt cgcgtccgcc ccgtaagccg tcatcatgtc gttcggatcg
     2101 acgccgttgt tcgccgactt cgacatcttc tcgaacggcc cgaccgtgac gggcaggccg
     2161 tccgagagga gcgtcgcttc gcggacgcgc ccccgttcgt cgcgcaccgt ctcgacctcc
     2221 gacgggttga agtagttctt ctgcgaagcg tcttcgccct cgcggtagaa ggtctcggcg
     2281 acgaccatgc cctgcgtcat caaatttttg accggctcgc cgaaactcac cagcccgatg
     2341 tcgcgcatga acttcgtcca gaagcgcgtg tagagcaggt gcatgacggc gtgctcgacg
     2401 ccgccgatgt actgatcgac cggcgtccag taggcggcga cctccttgtc gaacggcgcc
     2461 tgctcgttgc gcgggtcgat gtagcggaag aagtaccagg acgagtcgat gaaggtgtcc
     2521 atcgtgtccg tctcgcggcg ggcgcccgcg ccgccgcact tcgggcaggt cgcgttgacg
     2581 aactccgcga cgccggccag aggcgagcgg cccgcgcccg tgaactccgc gtgctcgggc
     2641 agcgtcaccg gcaagtcctt ttcggggacg ggcaccgtgc cgcacttctc gcaatagacg
     2701 acggggatcg gcgcgcccca gaagcgctgg cgcgagacgc cccagtcgcg cagccggtag
     2761 gtgactgccg ggtcgccgaa gcctttccgc ttcgcgtgct cggccatcac gcgcatggcg
     2821 tccgcggaga gcttgccgct ccactcgccg gagttgaccg tgatgccgta gtcggtgaag
     2881 gcgtgcggca tggcgaccga ctggtcttcg gcttccgggt cgtgcgggat ggcttcctgc
     2941 gcgtgcgaga tgcgctcgat gacgcggcgg acgggcaagc tgtacttctg cgcgaactcg
     3001 aagtcgcggt cgtcgtgcgc gggcacgctc atcaccgcgc ccgtgccgta ctccatgagc
     3061 acgtagttcg cggcccacac cgggatcgac tcgccgctga aagggttgac ggccagcagg
     3121 ccggtgttga tgccctcctt ctcctcctcc tcgccgggcg cgcgcgccgc cgccccgcgc
     3181 gccgcgcgga tgcgctcggc gaacctgcgc acgtcgccct tcagaggcga cagctcgacc
     3241 agcgggtgat cgacggccag cacgatggcg gtcgcgccgt agatggtgtc gatgcgcgtg
     3301 gtgaagacgc ggacgcgctc gtccgtgtcc ttgatctgga aatcgacgta ggcgccttcg
     3361 ctccgaccga tccagttgcg ctgcatcgtc aggacgcgct cgggccagcc cgcctcgatc
     3421 tcctccatgt ccgcgagcag ttgttcggca taggccgtga tgcgcaagaa ccactgctcg
     3481 atgtcgcgct tctcgaccgc cgcgccgcag cgccagcaga cgccgcccga cgactgctcg
     3541 ttcgacagc
//
LOCUS       DBNGOH010000142         2475 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1486662, whole genome shotgun sequence.
ACCESSION   DBNGOH010000142 DBNGOH010000000
VERSION     DBNGOH010000142.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2475)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2475)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2475
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1486662"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /gene="groEL"
                     /locus_tag="AC159P_02630"
     CDS             <1..192
                     /gene="groEL"
                     /locus_tag="AC159P_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014777260.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; many bacteria have multiple
                     copies of the groEL gene which are active under different
                     environmental conditions; the B.japonicum protein in this
                     cluster is expressed constitutively; in Rhodobacter,
                     Corynebacterium and Rhizobium this protein is essential
                     for growth; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HIR3694378.1"
                     /translation="NGKFEDLSAAGIIDPTKVVRTALQNAASIAGLLLTTDAAVTDAP
                     EPKRAAAPPMPGGGDDYDY"
     gene            573..>2475
                     /locus_tag="AC159P_02635"
     CDS             573..>2475
                     /locus_tag="AC159P_02635"
                     /inference="COORDINATES: protein motif:HMM:NF019344.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR3694379.1"
                     /translation="MIDLGTYKDRFAESGQRVLEHAIQESRRRDQNYIAVEHILNALS
                     VEEADLFNSTMRDLALDPRSVKVLIEKRLENSRYQNVNKAIRIAPETIDLFKKAMERA
                     RAQGRKTIEATDLFMALSQDENSAFMGVLRNLGANPENVIENVRARIRTREQEEEQYK
                     KKFELPPYLKHFGVSLNRLARMDKLPPTIGREQEIQQMIEILCHRERANSPMLVGEPG
                     VGKTAVVEGLARLIELEPEKVPSRLRGTHIVQLQMGGLVAGTMLRGMFEERIKGIIDE
                     VKERESLILFIDEAHTIIGAGAALGTSSDAANMFKTALARGEMRIIGATTNTEFKEYI
                     AEDEALARRFRQVKVAEPSLSETREILFGLRPRLERNYSVTISDDAINTALEMAPKYI
                     RNLHLPDKAIGWLDTAAVKVEINEPEAMVVKPEHVIDVISQESRIPRDMIFRDTTDRF
                     AHMEEDLGTRVIGQKDAIRAVAQRLRLNKGPLKESHYKPDGVLLFLGPTGVGKTELAK
                     AVAEFMFGDDEKMVRIDLSEYADGTIGIEKLIGMPRGIVGSERGGVLTEQLRENPYTV
                     LLLDEVEKASPTLMNLFLQAFDEGWLTDGRGKKVYLSDAIVIMTSNLGSESFKKYLKP
                     LGFGSDRRSD"
BASE COUNT          504 a          780 c          792 g          399 t
ORIGIN      
        1 aacggcaagt tcgaagacct ctccgcggcg ggcatcatcg atccgacgaa ggtcgtccgc
       61 acggcgttgc agaacgcggc ctccatcgcg ggcctgctgc tcacgaccga cgccgcggtg
      121 acggacgcgc ccgagccgaa gcgggccgcc gcgcccccga tgccgggcgg cggcgatgac
      181 tacgactact aaaaaccgta agccgtaaac cgtgaaccgt gataagagga gagcagtttt
      241 cttcttatca cggttcacgg tttacggctt acggctttcg agttgtcacg tgtcgcggcg
      301 agcggttcct tttttacaaa gggcctttct actggcttaa gttttgctgt gcgttcgtgt
      361 gctatgatga aggtgtccgg aagcgcgaca aagcacaacc gggccttcgc acctcgcatc
      421 taaaacaagg ccgtccgggc gggcgtcttt gtcgaaactg tttcgccagt ttgagagcac
      481 ggtttcaacg ctcgtcgaga gacgtgacgg ctttgccgga tgttgcgggt tcgaggtgca
      541 attgggccgc tcccgcgaaa gtaggaaaag caatgattga tcttgggaca tacaaagacc
      601 gtttcgcgga gagcggccag cgcgttttag aacacgccat ccaggagtcg cggcggcgtg
      661 accagaacta catcgccgtc gagcacatcc tgaacgccct ctccgtcgag gaggccgacc
      721 tcttcaactc gacgatgcgc gatctcgcgc tcgacccccg ctcggtcaag gtcttgatcg
      781 agaagcggct cgaaaactct cgttaccaga acgtcaacaa ggcgatccgc atcgcgcccg
      841 agaccatcga cctcttcaag aaggcgatgg agcgcgcccg cgcgcagggg cgcaagacca
      901 tcgaggcgac agacctcttc atggcgctct cgcaggacga gaacagcgcc ttcatgggcg
      961 tcttgaggaa cctcggggcg aaccccgaga acgtgatcga gaacgtgcgc gcccgcatcc
     1021 ggacgcgcga gcaggaggag gagcagtaca agaagaagtt cgagctgccg ccctacctca
     1081 agcacttcgg cgtcagcttg aaccggctgg cgcggatgga caagctgccg ccgaccatcg
     1141 ggcgcgagca ggaaatccag cagatgatcg aaatcctctg ccaccgcgag cgcgccaact
     1201 cgccgatgct cgtcggcgag ccgggagtgg gcaagacggc ggtggtcgaa ggtctcgcgc
     1261 gcctcatcga gcttgagccg gagaaggtgc cgtcgcgttt gcgcggcacg cacatcgtcc
     1321 agctccagat gggcggcctc gtcgccggga cgatgctccg cggcatgttc gaggagcgca
     1381 tcaagggcat catcgacgag gtgaaggagc gcgaaagcct catcctcttc atcgacgagg
     1441 cgcacaccat catcggcgcg ggcgcggcgc tcggcacctc ctcggacgcg gccaacatgt
     1501 tcaagacggc gctcgcgcgc ggcgagatgc ggatcatcgg cgcgaccacc aacaccgagt
     1561 tcaaggagta catcgccgag gacgaggcat tggctcgtcg cttccggcag gtcaaggtcg
     1621 cggagccttc gctctcggag acgcgcgaga ttctgttcgg cctgaggccg cggctcgaaa
     1681 gaaattattc cgtcaccatc tcggacgacg ccatcaacac ggcgctggag atggcgccga
     1741 agtacatccg caacctgcat ctgccggaca aggccatcgg gtggctcgac acggcggcgg
     1801 tgaaggtcga gatcaacgaa cccgaagcga tggtcgtcaa gcccgagcac gtcatcgatg
     1861 tgatctcgca ggagtcgcgc atcccgcgcg acatgatctt ccgcgacacg accgaccgct
     1921 tcgcgcacat ggaggaagac ctcggcaccc gggtcatcgg gcagaaggac gccatccgcg
     1981 ccgtcgccca gcggctgcgc ctgaacaagg ggccactcaa ggagtcgcac tacaagccgg
     2041 acggcgtgct cctcttcctc ggcccgaccg gcgtcggcaa gaccgagctg gcgaaggcgg
     2101 tcgccgagtt catgttcggc gatgatgaga agatggtgcg catcgacctg tcggagtacg
     2161 ccgacggcac catcggcatc gagaagctga tcggcatgcc ccgcggcatc gtcggcagcg
     2221 agcgcggcgg cgtcctgacc gagcagctcc gcgagaaccc gtacacggtc ctcctgctcg
     2281 acgaggtcga gaaggcgagc ccgacgctga tgaacctctt cctgcaggcc ttcgacgagg
     2341 gctggctgac ggacgggcgc ggcaagaagg tgtacctctc ggacgcgatc gtcatcatga
     2401 cgagcaacct cggcagcgag agcttcaaga agtacctcaa gccgctcggc tttggctcgg
     2461 accgccgctc ggaca
//
LOCUS       DBNGOH010000143         3146 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_387587, whole genome shotgun sequence.
ACCESSION   DBNGOH010000143 DBNGOH010000000
VERSION     DBNGOH010000143.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3146)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3146)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3146
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_387587"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(43..537)
                     /gene="coaD"
                     /locus_tag="AC159P_02640"
     CDS             complement(43..537)
                     /gene="coaD"
                     /locus_tag="AC159P_02640"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HIR3694380.1"
                     /translation="MRRAIYPGSFDPVTNGHLDIIGRGSKLFDEIVIAILVHPEKKPL
                     FTIDERREMLEEVLQETTADGCRLRVDFFQGLLVKYAVEQEADAIVRGIRAVSDYEYE
                     LQMALMNRRLEPRIETVFMMAAEDYSYVSSRLVKEVYQLGGTIHGLVPPSVEARMQRK
                     FGDG"
     gene            784..1224
                     /locus_tag="AC159P_02645"
     CDS             784..1224
                     /locus_tag="AC159P_02645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694381.1"
                     /translation="MSRLCMQVFAIALCFAAFAPAYGVAQERPQRRVVAAVEKVHVGD
                     MGADDEADRFRLLLEEQLTKKGFTVVRTEAEADAVLTGVLSVRVYDRKTEARVFVRLT
                     SRDGAQLWARDFGHKRLRINPFSLQEPTRRRAEEVAEAMRREFK"
     regulatory      1281..1356
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00521"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SAM riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00521"
     gene            1517..>3146
                     /locus_tag="AC159P_02650"
     CDS             1517..>3146
                     /locus_tag="AC159P_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012595771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HIR3694382.1"
                     /translation="MTDFLNTLKERIVVFDGAMGTSLQTQNLTLEDYGGAQFEGCPEY
                     LLISKPSAVEQVHAGFLEVGCDVIETDSFGGTPVVLAEYQIAHMAYELNFKAAELAKR
                     VAGDYATKGRPRWVAGSIGPGTKLPTLGHATFEELRDAYGVQVRGLVDGGADLLIVET
                     CQDLLQTKSALAAIFGYFKEIKRRIPVIAQVTIEVFGTMLNGTEISAALTALEPVPID
                     VIGMNCGTGPRHMTESIRYLCENAPLPVSVLPNAGLPEVKDGKQHYGETPESFTAQIV
                     HFARDFGVNVVGGCCGTTPEHLKSVVEAMRDITPKQRDARLVPSASSIYMQQPYVQDA
                     SFLVVGERVNASGSKKMRDLLNAEDWDGLVSLAREQEREGAHILDVNVDYVGRDGERD
                     MHALVSRLVTDVKIPLMLDSTEWQKMEAGLQHAGGKCILNSTNYEDGEPRFKKLIELA
                     QEYGAAVVVGTIDEQGMARGAEGKFEIAKRAYEQATEELGFPAHDIFFDPLALPISTG
                     IEEDRRNAAETIEALRRIKRELPGCLTILGVSNIS"
BASE COUNT          603 a          997 c         1048 g          498 t
ORIGIN      
        1 ggtagaacgc cgcccacgag cgcgcaagca gcggcgcgcc tctcagccgt cgccgaactt
       61 ccgctgcatg cgcgcctcga ccgagggcgg gacgagtccg tgaatcgtgc cgccgagctg
      121 gtagacctct ttgacgaggc gcgagctgac gtaggaataa tcctcggcgg ccatcatgaa
      181 gacggtctcg atgcgcggct ccagccgccg gttcatcaaa gccatctgca actcgtactc
      241 gtagtccgac acggcgcgga tgccgcgcac gatggcgtcc gcctcttgct cgacggcgta
      301 cttcacgagc agcccttgaa agaaatcgac gcgcaggcgg cacccgtccg ccgtcgtctc
      361 ttgcaggact tcttcgagca tctcgcgccg ctcgtcgatg gtgaagagcg gcttcttttc
      421 cgggtggacg aggatggcga tgacgatctc gtcgaagagc ttcgagccgc gcccgatgat
      481 gtcgaggtgg ccgttcgtca cggggtcgaa ggagccgggg tagatggcgc gccgcataag
      541 cgtgtggcag cagcaggggg tagcaagtcc gtggcgaaag taacacaaaa gcgggcggtc
      601 gagaaaattt atttatcgcg gccaacgccc ccggcgggtc ggccttctta gcagtcaacc
      661 cacgaccctt ttcagggttc acaggaatgg acgcgtcacg acacctcgca gggcaacggg
      721 cactctcccc ggcgccgccg gaagccgcca gcgtctccgg cctacgcaag aaaggagaac
      781 gagatgagtc gtttgtgcat gcaggtcttc gccatcgcct tgtgcttcgc ggccttcgcg
      841 cccgcctacg gcgtggccca ggagcggcca cagaggcgcg tcgtcgccgc ggtcgagaag
      901 gttcacgtcg gcgacatggg cgccgatgac gaggccgacc ggtttcgcct cctgctcgaa
      961 gagcagctca cgaaaaaagg attcaccgtc gtccgcaccg aggcggaggc ggacgccgtc
     1021 ctgacgggcg tgctctccgt tcgcgtgtat gacagaaaga ccgaggcgcg cgtcttcgtc
     1081 cggctcacgt cacgggacgg cgcgcagctc tgggccagag acttcgggca caagcggctg
     1141 cgcatcaacc ccttcagcct tcaggagccg acccgccgcc gcgccgagga agtcgccgaa
     1201 gcgatgcgcc gcgagttcaa atgagccgcc gaggagcgtg gcttgacacg cgcgcgcggg
     1261 cggtgtttca atacccgacg gtagacaagc gtggtgagtt aaggcgactt gcggccacgt
     1321 aaaataaaca gctaaacagc tcggcgcgtc tttccgaaga ccgacgcaac aaaggctctc
     1381 gatgaagaag ccccgcgttg tttggcgcgg ggcgattttt ttgaatagta ggcagtaggc
     1441 ggtaggcagt aggcagttaa gaaaactgct tttgctgcct actgcctact gcctaccgcc
     1501 tactgcctac tgaaatatga ctgattttct caacacactg aaagagcgca tcgtcgtctt
     1561 cgacggggcg atggggacta gtctccagac gcagaacctg acgctggagg actacggcgg
     1621 cgcgcagttc gagggctgcc cggagtacct gctcatcagc aagccgtcgg cggtcgagca
     1681 ggtgcacgcg ggcttcctcg aagtcggatg cgacgtgatc gagaccgact cgttcggcgg
     1741 cacgcccgtc gtgctcgccg agtaccagat cgcgcacatg gcctacgagc tgaacttcaa
     1801 ggcggcggag ctggcgaagc gcgtggcggg cgactacgcg acgaaggggc gcccgcggtg
     1861 ggtcgcaggc tccatcgggc cggggacgaa gctgccgacg ctcggccacg ccaccttcga
     1921 agaactccgc gacgcctacg gcgtgcaggt gcgcggcctc gtggacggcg gcgccgatct
     1981 gcttatcgtc gagacgtgcc aggacttgtt gcagaccaag tccgcgctcg ccgccatctt
     2041 cggttacttc aaggagatca aaagacgcat ccccgtcatc gcgcaggtca ctatcgaagt
     2101 cttcgggacg atgctgaacg ggacggagat cagcgccgcg ctgacggcgc tcgaaccggt
     2161 ccccatcgat gtcatcggga tgaactgcgg caccggcccg cgccacatga cggagagcat
     2221 ccgctacctc tgcgagaacg cgccgctgcc cgtctcggtg ctgccgaacg cggggctgcc
     2281 cgaagtcaag gacggcaagc agcactacgg cgagacgccg gagtcgttca cggcgcagat
     2341 cgtccacttc gccagagact tcggcgtcaa cgtcgtcggc ggctgctgcg gcacgacgcc
     2401 ggagcacctg aagtccgtgg tcgaggcgat gcgcgacatc acgccgaagc agcgcgacgc
     2461 gcggctcgtg ccgtcggcct cttcgatcta catgcagcag ccgtacgtgc aggacgcgtc
     2521 gttcctcgtc gtcggcgaac gcgtcaacgc gagcggctcg aagaagatga gggacttgct
     2581 gaacgccgaa gactgggacg gcctcgtctc gctggcgcgc gagcaggagc gcgagggcgc
     2641 gcacatcctc gacgtgaacg tggactacgt gggccgggac ggcgagcgcg acatgcacgc
     2701 gctcgtctcg cggctcgtga cggacgtgaa aatcccgctc atgctcgact cgaccgagtg
     2761 gcagaagatg gaggcgggcc ttcagcacgc gggcggcaag tgcatcctca actcgacgaa
     2821 ctacgaggac ggcgagccgc gcttcaaaaa gctgatcgag ctggcgcagg agtacggcgc
     2881 cgcggtcgtc gtcgggacga ttgacgagca gggaatggcg cgcggggcgg aagggaagtt
     2941 cgagatcgcg aagcgcgcgt acgagcaggc gaccgaagaa ctcggcttcc cggcgcacga
     3001 catcttcttc gacccgctcg cgctccccat ctcgaccggc atcgaggaag accgccgcaa
     3061 cgccgccgag accatcgagg cgctgcggcg gataaagcga gagctgcccg gctgcctcac
     3121 catcctcggc gtctcgaaca tctcgt
//
LOCUS       DBNGOH010000144         2276 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1670501, whole genome shotgun sequence.
ACCESSION   DBNGOH010000144 DBNGOH010000000
VERSION     DBNGOH010000144.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2276)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2276)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2276
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1670501"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(160..762)
                     /locus_tag="AC159P_02655"
     CDS             complement(160..762)
                     /locus_tag="AC159P_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HIR3694383.1"
                     /translation="MITILGTALGAAWTAGINLYATVAVLGLLQRFGFVSRLPGGLEV
                     LDNWWIIGVALFLYAVEFFADKIPYVDTVWDAVHTFIRVPAGAVIALAATTDLDPSIQ
                     VVALLIGGGLALSTHGTKATVRAGANLSPEPLTNWTLSLVEDVFAVGAVVLAVLHPVA
                     VLVLILIFLLILAWVLPKVVRRLRKMVAAGLAFFRGKAPA"
     gene            complement(872..2248)
                     /locus_tag="AC159P_02660"
     CDS             complement(872..2248)
                     /locus_tag="AC159P_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HIR3694384.1"
                     /translation="MARKSILVVDDEKPQREILEEILSGEGYDVTPAASGEAALRIAR
                     ERRFDLALTDLKMTGMDGIELLQKLLAYDSSIIVILLTAHGSIDSAKEALRRGAFDYL
                     EKPFDQETLLGTIRRALSKLDALDQEIISASPEMEAVKKMILKVARSNSTVLIRGESG
                     TGKELIARAIHNQSPRTTEMFQAVNCAAINENLLESELFGHEKGSFTGAHAEKKGLFE
                     VADKGTLFLDEIAELDIGMQAKLLRALQEREIRRVGGTRAIKIDVRVVAATNRDLRAM
                     VGDGRFRDDLYYRINVLSVDVPPLRERREDIPVLMDFFLKKHTRNTSRLVRGLTPDAR
                     RVMLDYAWPGNVRQLESAIERAILLAESDLIAVEDLPLEVRQESRPAAETGFKLPADG
                     ISFEDVERNLILQAMERTDYNITKAAKLLGLTFRTLQYRLEKFGIKRADKHGAAGEQE
                     EEEEKDEE"
BASE COUNT          389 a          718 c          756 g          413 t
ORIGIN      
        1 ccagtctcat ctccgacctc caaaaaattt ccgtcaccac actcgcccct tgaacgagcc
       61 tgcgcgccgc tgcttgcggc gtgcggtctc gaaaactctc gcggcggccc ctcgacgtta
      121 cgcggactgg cggcggcggg cgacgtggct tcgcacaggt caggccggtg ccttcccacg
      181 gaagaaggcg agcccggcgg cgaccatctt ccgcaggcgg cggacgacct tcggcaacac
      241 ccacgcgagg atcagcagga agatcagaat cagcacgagc accgcgaccg ggtggagaac
      301 ggcgagcacg acggcgccga cggcgaacac gtcttcgacg agcgagagcg tccagttcgt
      361 caacggctcg gggctgaggt tggccccggc gcgcacggtc gccttcgtgc cgtgcgtcga
      421 gagcgcgagt ccgccgccga tgagcagcgc gacgacctgt atcgaggggt cgaggtcggt
      481 cgttgccgcg agcgcgatga cggctccggc gggcacgcgg atgaacgtgt gcaccgcgtc
      541 ccagaccgtg tccacgtaag gaatcttgtc ggcgaagaat tcgaccgcgt agaggaagag
      601 cgcgacgccg atgatccacc agttgtccag aacttccagc ccgcccggca gccgcgagac
      661 gaagccgaag cgctgcaaca gccccagcac cgccaccgtc gcgtagaggt tgatgcccgc
      721 agtccacgcc gcgccgagcg ccgtgccgag tatggtaatc acgtccatcg agatcgaaag
      781 tcagaagtca gaagtcagaa gtcggaacgc cgaagtatga aacgaagtcg aattgtgttt
      841 agttcatact tctgcgttcc gacttcatcc ttcattcttc atccttctct tcttcctctt
      901 cctgctcgcc cgcggcgccg tgcttgtcgg cgcgtttgat gccgaacttc tccaggcggt
      961 attggagggt gcggaaggtg aggccgagca gcttggccgc cttcgtgatg ttgtagtcgg
     1021 tgcgctccat cgcctggagg atgaggttgc gctccacgtc ctcgaagctg atgccgtcgg
     1081 cggggagctt gaagccggtc tcggcggcgg ggcgcgactc ctgccggact tcgagcggca
     1141 ggtcttcgac cgcgatgagg tcgctctcgg cgagcaggat ggcgcgctcg atggccgatt
     1201 cgagctgccg cacgttgccg ggccacgcgt agtcgagcat gacgcgacgg gcgtcgggcg
     1261 tcaggccgcg cacgagccgc gaagtgttgc gcgtgtgctt cttcaaaaag aagtccatca
     1321 ggacggggat gtcttcgcgc cgctcgcgca aaggcggcac gtccaccgag aggacgttga
     1381 tgcggtagta caggtcgtcg cgaaagcgac cgtcgccgac catcgcgcgc aggtcgcggt
     1441 tggtggcggc cacgacgcgc acgtcgatct tgatggcgcg cgtgccgccg acgcggcgta
     1501 tctcgcgctc ctgcaaagcg cgcagcagtt tggcctgcat cccgatgtcg agttcggcga
     1561 tctcgtcgag gaagagcgtg cccttgtctg cgacttcgaa gaggcccttc ttctcggcat
     1621 gcgcgccggt gaaggagcct ttctcgtggc cgaacagctc ggactccagc aggttctcgt
     1681 tgatggccgc gcagttgacg gcctggaaca tctcggtcgt gcgagggctt tggttgtgga
     1741 tggcgcgcgc gatcagctcc ttgcccgtgc cgctttcgcc gcggatgagg acggtcgagt
     1801 tcgagcgcgc gaccttgagg atcatcttct tgacggcctc catctcgggc gaggccgaga
     1861 tgatctcctg atcgagcgcg tcgagcttgg agagcgcgcg gcggatggtg ccgagcagcg
     1921 tctcctgatc gaagggcttt tcgaggtagt cgaaggcgcc gcgccggagc gcctccttcg
     1981 ccgagtcgat ggagccgtgc gccgtcagca ggatgacgat gatcgaagag tcgtaggcca
     2041 gcagcttctg caacagctcg atgccgtcca tcccggtcat cttcaagtcc gtcagcgcga
     2101 ggtcgaagcg ccgttcgcgc gcgatcctga gcgccgcctc gcccgaggcg gcgggcgtca
     2161 cgtcgtagcc ctcgcccgac agaatctctt ccagaatctc ccgctgcggt ttctcgtcgt
     2221 caaccacgag tatgctttta cgcgccattc ataaattaag gcagaagtcg gaacgc
//
LOCUS       DBNGOH010000145         2814 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1121170, whole genome shotgun sequence.
ACCESSION   DBNGOH010000145 DBNGOH010000000
VERSION     DBNGOH010000145.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2814)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2814)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2814
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1121170"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            116..>2814
                     /locus_tag="AC159P_02665"
     CDS             116..>2814
                     /locus_tag="AC159P_02665"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HIR3694385.1"
                     /translation="MGLRHPPKGYVPPVARKRGARVVKFEEPEVGFFRRWLRRLTRPV
                     VILPVVFLTAVVVGVLGYYYYIFSARIDRLLRGEVFTRTAGIYAAPKQLRAGETIAVE
                     DLVGRLRRAGYVERAQQADDSRGRYALAGTTVEIEPSKDSMIDGTRQFPRLRAQFEKG
                     GKRVAALVNMDGGARMQSAWLEPEQISSVTGAEREKRKVIGFADIPESLRKAIVTTED
                     RTFFDHYGINFRGILRALIRRYDADPNSPIARQGGSSITQQLVKNILLSPEYSLKRKV
                     AEAYMSLIIETRLSKEEIFALYCNEAYLGQRAGCSIKGFGEASEAYFNKDVTALSLPE
                     SALLASLIRSPNRYNPYRHPQVATERRNQVLRDMAATGAITEVEAAQAMATELKVAPQ
                     KGRIDVSEAPYFVDYAQQQLSDMLADTSAAERLRIYTTVDMELQRAAYAAVVKQLAAL
                     DKVQSRRFAPGTVQAALVAMNAQTGEVVAMVGGRDYEKSQLNRATEAMRQPGSVFKPF
                     VYAAALNTAFDPIPRVITAATTYKDAPQTFQTNGQEYSPGNFGEHYSNKDVTVRDALV
                     KSLNVVTVELAQDVTIGRVMNLAAKAGLPKPKQNYLAHALGTNEATPLQVASAYTAFA
                     QEGKRASPVVINRVTTGGGRTVAAPTTQRNEVLKPEVAYIMTSMMKDVVNRGTAAKVR
                     ARGFKADVAGKTGTSRDGWFAGYTPNLVCVVYVGFDDGSQLGMTGADSALPIWADFMT
                     AALQAHPEWAGDWQMPGGVEQVEIDAQTGALPTETSTAKRTELFITGTAPTAPSEDAP
                     EDTDPLGDEIEGGDDALPQSTPYAIEVPPLPEATPPSSRTRTAPGDDRPGSTQSGDPT
                     RPAYAPQAPAAARPTNVSEEYAPAPRGETRETRVELGRDGRRRL"
BASE COUNT          492 a         1008 c          933 g          381 t
ORIGIN      
        1 ggtaaatagt gggtgagagc gcggggcggt cgagagctga tcgccgcgcc gcgcgccgcg
       61 cgccgtttta tttcgattta ctattcacca tttaccattt accatttacc gcgacatggg
      121 tcttcgccat ccaccgaaag gctacgtgcc gcccgtcgcg cgcaagcgcg gggcgcgggt
      181 cgtcaagttc gaggagccgg aggtcggctt cttccgacgg tggttgcgcc gcctcacgcg
      241 gcccgtcgtc atcctgcccg tggtcttcct gaccgccgtc gtagtcggcg tcctcggcta
      301 ctactactac atcttctcgg cccgcatcga ccggctgctg cgcggcgagg tcttcacgcg
      361 cacggcgggg atttacgccg cgccgaagca gttgcgcgcg ggcgagacga ttgccgtcga
      421 agatttggtg gggcggctca ggcgcgcggg ctacgtcgag cgcgcgcagc aggcggacga
      481 ctcgcgcggg cgctacgcgc tcgcgggcac gaccgtcgag atcgagccga gcaaggactc
      541 gatgatcgac ggcacgcggc agttcccgcg gctgcgcgcg cagttcgaga agggcggcaa
      601 aagggtcgcg gcgctcgtga acatggacgg cggcgcgcgc atgcagtcgg cgtggcttga
      661 gccggagcag atcagctcgg tgacgggcgc cgagcgcgag aagcgcaagg tcatcggctt
      721 cgccgacatc cccgagagcc tgcgcaaggc catcgtcacg accgaagacc gcaccttctt
      781 cgaccactac ggcatcaact tccgcggcat cctgcgcgcg ctcatccgcc gctacgacgc
      841 cgacccgaac tcgcccatcg cgcggcaggg cggctcgtcc atcacgcagc agctcgtcaa
      901 gaatatcctg ctctcgcccg agtactcctt gaagcgcaag gtggccgaag cctacatgtc
      961 gctcatcatc gagacgcgcc tgtcgaagga ggaaatcttc gcgctctact gcaacgaggc
     1021 ttacctcggg cagcgcgcgg gctgctcgat caagggcttc ggcgaggcga gcgaggcgta
     1081 cttcaacaag gacgtgacgg cgctcagcct gcccgagtcg gcgctcctcg ccagcctcat
     1141 ccgcagcccg aaccgctaca acccttaccg ccacccgcag gtcgccaccg agcgccgcaa
     1201 ccaggtcttg agggacatgg ccgcgaccgg cgcgatcacc gaggtcgagg cggcgcaggc
     1261 gatggcaacc gagctgaagg tcgcgccgca aaagggccgc atcgacgttt cggaggcgcc
     1321 ctacttcgtc gattacgcgc agcagcaact ctccgacatg ctcgccgaca ccagcgccgc
     1381 cgagcggctg cgcatctaca cgaccgtgga catggagttg cagcgggcgg cctacgcggc
     1441 ggtcgtcaag cagctcgccg cgctcgacaa ggtgcagtct cgaaggttcg cgccggggac
     1501 ggtgcaggcc gcgctcgtgg cgatgaacgc gcagaccggc gaggtcgtgg cgatggtcgg
     1561 cgggcgcgac tacgagaaga gccagctcaa ccgcgcgacc gaagcgatgc gccagcccgg
     1621 ctcggtcttc aagcccttcg tctacgccgc cgcgctcaac accgccttcg acccgatccc
     1681 gcgcgtcatc accgccgcga ccacctacaa ggacgcgccg cagaccttcc agaccaacgg
     1741 gcaggagtat tcgcccggca acttcggcga gcactattcg aacaaggatg tcaccgtgcg
     1801 cgacgcgctc gtcaagagcc tcaacgtcgt cacggtcgag ctggcgcagg acgtgaccat
     1861 cgggcgcgtg atgaatctgg cggcgaaggc gggcctgccc aagccgaagc agaactatct
     1921 ggcgcacgcg ctcggcacga acgaggcgac gccgctgcaa gtcgcctcgg cctacacggc
     1981 cttcgcgcag gaaggcaagc gcgcctcgcc cgtcgtcatc aaccgcgtca cgacgggcgg
     2041 ggggcgcacg gtcgccgcgc ccaccacgca gcgcaacgaa gtgctcaaac ccgaggtcgc
     2101 ctacatcatg acctcgatga tgaaggacgt ggtgaaccgc ggcacggcgg cgaaggtgcg
     2161 cgcgcgcggc ttcaaggccg acgtcgcggg caagaccggc acctcgcgcg acggctggtt
     2221 cgcgggctac acgccgaacc tcgtctgcgt cgtctacgtc ggcttcgacg acggctcgca
     2281 gctcggcatg acgggcgccg actcggccct cccgatctgg gccgacttca tgaccgccgc
     2341 cctccaggcg caccccgagt gggccggcga ctggcagatg cccgggggcg tcgagcaggt
     2401 cgagatcgac gcgcagaccg gcgcgctccc cacggagact tcgaccgcca agcgcacgga
     2461 gctgttcatc accggcaccg cgccgaccgc gccgagcgaa gacgcgcccg aagacaccga
     2521 cccgctcggc gacgagatcg aaggaggcga cgacgcgctg ccgcagtcaa cgccctacgc
     2581 gattgaagtg ccgccgctgc ccgaagccac gccgccgtct tcgcgcacgc gcaccgcgcc
     2641 cggcgacgac cggcccggct cgacgcaaag tggcgacccg acgcgccccg cctacgcccc
     2701 gcaggcaccg gcggccgcgc gcccgacgaa tgtttcggaa gagtatgctc ccgcccccag
     2761 gggcgagacg cgtgaaacgc gcgtcgaact cggccgcgac ggacgccgcc gcct
//
LOCUS       DBNGOH010000146         2211 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1670702, whole genome shotgun sequence.
ACCESSION   DBNGOH010000146 DBNGOH010000000
VERSION     DBNGOH010000146.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2211)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2211)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2211
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1670702"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..259
                     /locus_tag="AC159P_02670"
     CDS             <1..259
                     /locus_tag="AC159P_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate ligase domain-containing protein"
                     /protein_id="HIR3694386.1"
                     /translation="TEDPESILADIEVGLKSAGKPYLKLMDRRAAIRHAVAEARAGDL
                     VLIAGKGHEDYQIIGAERIRFDDREVAAEALAEVGRMKSEE"
     gene            368..1279
                     /locus_tag="AC159P_02675"
     CDS             368..1279
                     /locus_tag="AC159P_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HIR3694387.1"
                     /translation="MKVIVTGSTGLVGQALIRSLMSEGHGVTRLVRGGAQTFRAPNSV
                     AVNWDPERGQIDAGELEGHDAAVHLAGEPVAEGRWTDEKKRRILESRVKSTRLLAETL
                     ASLKDKPKVLVSASATGFYGNRGAEVLVEESASGEDFLSEVCREWEKAALAASQAGIR
                     VVHTRIGIVLSGEGGALKKMLTPFKLGVGGRVGSGEQYMSWITLDDLVGVIRHVIENE
                     HVRGPVNAVAPRAVTNAEFTKALGHVLGRPTVLPVPAFAARLAFGEMADAVLLAGARV
                     KPARLEETGYEFKHTEIEGALRHVLKK"
     gene            1344..1787
                     /locus_tag="AC159P_02680"
     CDS             1344..1787
                     /locus_tag="AC159P_02680"
                     /inference="COORDINATES: protein motif:HMM:NF025006.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HIR3694388.1"
                     /translation="MVRTTSVWFVVLLLALGAAAQTERKTSAPERGNVGKIKGVVLDK
                     NDARVAGARVGISNAKSRRAVASNEEGEFEVELPAGVYRVRVKANGFRAFVLWPFEVK
                     PGVTETIDVHLEVATSPGLVPAVEPDPPAHSSAKTGAKVAPSKPW"
     gene            1784..>2211
                     /locus_tag="AC159P_02685"
     CDS             1784..>2211
                     /locus_tag="AC159P_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002701483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694389.1"
                     /translation="MTHEAGAPRPRWMKKLLVAAGVYNIAWGALVVLFPLMLFRWLGM
                     PEPNYPQIWQCVGMVVGVYGVGYMIAATDPVRHWPIVLVGLLGKLFGPVGFIRAAWNG
                     ELPWAFGVVNITNDLVGLVPFTLILVHAYKSSRQRAAGSR"
BASE COUNT          380 a          639 c          840 g          352 t
ORIGIN      
        1 cacggaagac ccggagtcga ttctcgcgga catcgaggtc ggcctgaagt cggcggggaa
       61 gccctacctt aagctcatgg acaggcgcgc ggcgattcga cacgcggtcg cggaggcgcg
      121 cgcgggcgac ctcgtgctga tcgcgggcaa gggacacgag gactatcaaa tcatcggtgc
      181 cgagcggatt cgcttcgacg acagggaggt ggcggcggag gcgctggccg aagtcggaag
      241 gatgaagtcg gaagaatgaa gtcggaagga tgaagtcgga acgcagaacg cggaacgaca
      301 ggcggcctgt tttttattca tcgttctgcc ttctgccttc tgcctttttt aaaacggagg
      361 tgggagcttg aaggtcatcg tcacgggttc gacgggtctc gtcggtcagg cccttattcg
      421 ttcgctgatg tcggaaggtc acggcgtgac gcggctcgtg cgcggcggcg cgcagacgtt
      481 ccgcgcgccg aactcggtcg cggtgaactg ggacccggag cgcggccaga tagacgcggg
      541 cgagctggag gggcacgacg cggcggtgca tctcgcgggc gagccggtcg ccgaggggcg
      601 ttggacggac gagaagaagc ggcgcatcct tgagagccgc gtcaagagca cgcgcctgct
      661 ggcggagacg ctggcgagtt tgaaagataa accgaaggtg ctggtcagcg cgtcggcgac
      721 gggtttctac ggcaaccgcg gcgcggaggt gctggtcgaa gagagcgctt cgggcgaaga
      781 ctttctgagc gaggtctgcc gcgagtggga gaaggcggcg ctcgcggcga gtcaggcggg
      841 catccgcgtc gtgcacacgc gcatcggcat cgtgctgagc ggcgagggcg gcgcgctcaa
      901 gaagatgctg acgccgttca agctcggcgt cggcggacgt gtcggcagcg gcgagcagta
      961 catgagctgg atcacgctcg acgatttggt gggtgtcatc aggcacgtga tcgagaacga
     1021 gcacgtgcgc ggcccggtca acgccgtcgc gccgcgggcg gtgacgaatg cggagttcac
     1081 gaaggcgctg gggcacgtgc tcggtcgccc gaccgtgctg cccgtcccgg cgttcgccgc
     1141 gcgcctcgcc ttcggcgaga tggccgacgc ggtgctgctc gcgggcgcgc gcgtcaagcc
     1201 cgcgcggctg gaagagaccg gctacgaatt caagcacacg gagatcgaag gggcgctgcg
     1261 gcacgtgctc aaaaagtgag cgggcgggga aacagtccgc gcgcgtcgcg gcgtcgaagg
     1321 tgtgccatgt gagggaggtg tctatggtca ggacgacatc cgtctggttc gtcgtgttgc
     1381 tcctcgcgct cggcgcggcg gcgcagacgg agcgtaagac ttccgcgccc gagcgtggga
     1441 acgtgggcaa gatcaagggc gtcgtgctcg acaaaaacga cgcacgggtg gcgggggcga
     1501 gggtcggcat cagcaacgcc aaatcgaggc gcgcggtagc gtcgaacgag gaaggcgaat
     1561 tcgaggtcga gctgcccgcc ggcgtttacc gcgtcagggt caaagcgaac ggctttcgag
     1621 cgttcgtgct ctggcctttc gaagtcaaac ccggcgtgac ggagacgata gacgtgcatc
     1681 tggaggtcgc gacgagtccc ggcctcgtac ccgccgtgga gcctgacccg cctgcgcact
     1741 cctccgcgaa gacgggggct aaagtcgcgc cgtcgaaacc gtggtgacgc acgaggcggg
     1801 cgcgccgcgg ccccgctgga tgaagaagct gctcgtcgcg gcgggcgttt acaacatcgc
     1861 gtggggcgcg ctggtggtgc tgttcccgct gatgctgttc cgctggctcg ggatgccgga
     1921 gccgaactac ccgcagatct ggcagtgcgt cgggatggtg gtcggcgtgt acggcgtcgg
     1981 gtacatgatc gcggcgacag accccgtgcg gcactggccg attgtgctgg tcgggttgct
     2041 gggcaaactc ttcgggccgg tcggcttcat ccgcgccgcc tggaacggcg agctgccgtg
     2101 ggccttcggc gtcgtcaaca tcaccaacga cctcgtcggg ctcgtcccgt tcacgctgat
     2161 tctcgtccac gcgtacaaaa gcagcaggca gcgggcagcg ggcagcaggc a
//
LOCUS       DBNGOH010000147         2246 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1487264, whole genome shotgun sequence.
ACCESSION   DBNGOH010000147 DBNGOH010000000
VERSION     DBNGOH010000147.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2246)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2246)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2246
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1487264"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..724)
                     /locus_tag="AC159P_02690"
     CDS             complement(<1..724)
                     /locus_tag="AC159P_02690"
                     /inference="COORDINATES: protein motif:HMM:NF025701.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease complex subunit PrcB family protein"
                     /protein_id="HIR3694390.1"
                     /translation="MSHVTSIVIVCAALALTGAAPAACGSNQNRTMNVNRVIVPTPTA
                     ETPSPTPTPEAQRPAGGEGMKVLAAGQYGKVEEPFLVVAREAKVYGELRALAEGLPEL
                     GADFFGKHAVVAAFAGTRNSGGYGVEIRRGADKRLVVEETTPPEDAMTTMALTQPFKV
                     VAVEVGEEESVEFDLGGARRSSTRWRPYAVTSGEFDSGGGFAGRHEKLTLAGTLRVAR
                     LDRLVTVLFELKGEGGTKERTLR"
     gene            complement(737..1984)
                     /locus_tag="AC159P_02695"
     CDS             complement(737..1984)
                     /locus_tag="AC159P_02695"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014101481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HIR3694391.1"
                     /translation="MNVLVLNCGSSTVKFRLIATDLDRIAEDTDERLARGLIERIGGE
                     ALITLHAAGREARRTTAPVRDTRAAVEQIIRWVTSEGSGIGAVKSVADVHAVGHRVVH
                     GGERFTQSVLITDEVLRGIEDCIELAPLHNPANIRGIEAAREVFGPGLPQAAVFDTAF
                     HQTLPEQAYLYALPYQWYRRHRVRRYGFHGTSHRYVAYRNRQLTSRSREGTKIVTLHL
                     GNGASACAIDGGSSVDTSMGFTPLEGLVMGTRSGDIDPAIIDYITAKEGLTPQEVDSI
                     LNKQSGLIGISGLTNDMRELLDEERESGDRRARLAIEIFCYRVRKYVGAYLAALGGSD
                     ALIFTGGIGENSPEIRARVCEGLAWMGVELDPARNADTVARREGLVSREGSRLGVYVI
                     PTDEELLIARDTVRSVRGVRQRY"
BASE COUNT          323 a          774 c          763 g          386 t
ORIGIN      
        1 cgcgcagcgt gcgctccttc gtcccgcctt cccctttcaa ctcgaagagc accgtcacga
       61 gcctgtcgag gcgcgcgacc cggagggtgc ccgcgagcgt cagcttctcg tggcgcccgg
      121 cgaagccgcc gcccgaatcg aactcgcccg acgtgacggc gtacggtcgc cagcgcgtcg
      181 agctgcggcg cgcgccgccg aggtcgaact cgaccgactc ctcctcgccg acctcgacgg
      241 cgacgacctt gaagggctgc gtgagcgcca tcgtcgtcat cgcgtcttcg ggcggcgtcg
      301 tctcctcgac gacgaggcgc ttgtcggcgc cgcgcctgat ctcgacgccg tagccgcccg
      361 agttgcgcgt gcccgcgaag gcggcgacga ctgcgtgctt cccgaagaag tccgcgccca
      421 gctccggcag cccttcggcg agcgcgcgca gctcgccgta gaccttcgcc tcgcgcgcga
      481 cgacgaggaa aggctcctcg accttcccgt actgccctgc ggccagaacc ttcatccctt
      541 cacctcccgc cgggcgctgc gcctcgggcg tcggcgtcgg cgacggggtc tccgccgtcg
      601 gtgtcggcac gatcactctg ttcacgttca tcgtcctgtt ctgattcgac ccgcacgcgg
      661 cgggtgccgc gcccgtgagc gccagcgcgg cgcagacgat gacgatgctt gtgacgtggc
      721 tcatcttcct tgcacctcag tatctctgcc gcacgccgcg cacggagcgc accgtgtcgc
      781 gcgcgatgag cagttcctcg tcggtcggga tgacgtaaac gccgagcctt gagccttcgc
      841 ggctgacgag tccctcgcgc cgcgcgaccg tatcggcgtt gcgcgcgggg tcgagttcga
      901 cgcccatcca cgccagcccc tcgcagacgc gcgcgcgtat ctcgggcgag ttctcgccga
      961 tgccgcccgt gaagataagc gcgtccgagc cgccgagcgc ggccaggtag gcgccgacgt
     1021 acttgcggac gcggtagcag aaaatctcga tggcgagccg cgcgcgccgg tcgcccgact
     1081 cgcgctcttc gtccaacagc tcgcgcatgt cgttcgtcag accggagatg ccgatgaggc
     1141 ccgactgctt gttcaggatc gagtccacct cctgcggcgt caggccctcc ttggcagtga
     1201 tgtagtcgat gatggccggg tcgatgtcgc ccgagcgcgt gcccatcacg agcccctcga
     1261 gcggcgtgaa gcccatcgag gtgtccaccg agctgccacc gtcgatcgcg caggccgacg
     1321 cgccgttgcc gagatgcagg gtgacgatct tcgtcccttc gcgactgcgg ctggtgagct
     1381 gtcggttgcg gtaggcgacg tagcggtgcg acgtgccgtg gaagccgtag cggcggacgc
     1441 ggtggcgcct gtaccactgg taggggagcg cgtagaggta ggcttgttcg ggcagcgtct
     1501 ggtggaaggc cgtgtcgaag acggcggcct gcggcagccc cggcccgaag acttcgcgcg
     1561 ccgcctcgat gccgcggatg ttcgccgggt tgtggagcgg cgccagctcg atgcagtcct
     1621 cgatgccgcg cagcacctcg tccgtgatga gcacggactg cgtgaagcgc tcgccgccgt
     1681 ggacgacgcg gtggccgacc gcgtgcacgt ccgcgacgct cttcacggcc ccgatgcccg
     1741 acccttcgga agtgacccaa cggatgatct gctcgaccgc cgcgcgcgtg tcgcgcaccg
     1801 gcgccgtcgt gcgccgcgcc tcgcggcccg ccgcgtgcaa cgtgatgagg gcctcgcccc
     1861 cgatgcgctc gatcagcccg cgcgcgagcc gctcgtccgt gtcctcggcg atgcggtcga
     1921 ggtcggtcgc gatgagccgg aacttgacgg tcgagctgcc gcagttgagg acgagaacgt
     1981 tcatgtgtga atggtaaatg gtaaatggta aatggtaaat ggcggttgag tggcgccggg
     2041 tgttgcgtga cgcattgaac gtctcagagg aaatggcttt ttctattcac catttgccat
     2101 tcaccattca ccgctttcac agatgtttca ggaaggcttc cgggcggttc aaaaagtcgc
     2161 gcgtcagcga gacgtgctcg acctcgtcgt aggcgacctc gcgcaccggc agcttgtcga
     2221 agccgaggat ggcggcgccg gggaag
//
LOCUS       DBNGOH010000148         3126 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_204410, whole genome shotgun sequence.
ACCESSION   DBNGOH010000148 DBNGOH010000000
VERSION     DBNGOH010000148.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3126)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3126)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3126
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_204410"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            192..1550
                     /locus_tag="AC159P_02700"
     CDS             192..1550
                     /locus_tag="AC159P_02700"
                     /inference="COORDINATES: protein motif:HMM:NF012471.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HIR3694392.1"
                     /translation="MRNPSLNPSPKARAAVVALILLLGAQPTAQTPRELFAEWQRHRL
                     TTLDPALVKHQDLKKYLAALKARGVPVAEVGRSLAGREIYRMEFGRGPVKVFMWSQMH
                     GDEPTATSTLVDLFAYLHSNRGVAWVKAVEEKLTLRAVPMLNPDGAELYRRRNLQSID
                     INRDARTLTTPEGRLLKKLRDEWQPDIGFNLHNQNPRTAVGDTGKQATISLLAVPSDA
                     SGADTPGRVRNKRLCAVMVEALAPFIRGHVGRYDDTFNPRAFGDLISQWGTPVILVET
                     GALKGTSGHDLAGLNFVALSAALRSLADGSAEGANPAVYDALPLNAGGTIYDLVVRGA
                     TIVSRAAPGGVRVQPFAADIAVNVERGRGDAARGGRTTVQDVGDLSVYKGLEEVDARD
                     YYVIAAAGELRTGSDAALLFYRKADSSAVKWDAPDIEKRFPPEAVFRGGQWVRRGKLP
                     VE"
     gene            1763..>3126
                     /locus_tag="AC159P_02705"
     CDS             1763..>3126
                     /locus_tag="AC159P_02705"
                     /inference="COORDINATES: protein motif:HMM:NF024583.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HIR3694393.1"
                     /translation="MSIEDSISQPAEERFRAVLEQSPYSIQIMSPDGRTLRVNRAWEE
                     LWGVKLEQIAGYNMLEDRQLVERGIMPLIRRAFEGEAVELPLILYDPEETVPGATRHR
                     DARRWTKAVAYPIKDAAGRVLEVVLMHEDVTARVLAEEENARLAAEAEARRTRQEADD
                     RFLAEATTALASSLDYETTLSTLARLAVPHFADWCGVDIADEGGTLRRLAVAHVDPAK
                     VAWARELQQRYPADPSAPRGSYNVLRTGESEFYPDITDEMLVAAAHDEEHLAALRAME
                     FRSAMIVPLKTRDRVLGIIVFVNTESGRRHAPADLAFAEDLAGRAALAVENARLYRAE
                     QQTRKAAEQTFDRLARLQAISSALSQALTQQQVAAAVIEQGISSLGAHAGSVVALDDD
                     GANLDLVTTVGFPPEVVEGWKRFALETPVPLADAARERLPVLVETFANYSERYPMLGP
                     MASIT"
BASE COUNT          519 a         1126 c         1042 g          439 t
ORIGIN      
        1 cacgatgcag cgtccgtcga agtgaacagt agatttccgt cacacgatgc cctatgctgc
       61 acgagttccg cggcccgctt gttcgagcgg ggacgaacca taacacagac gtttcgagtg
      121 tcagaagccg acgagccggc gacgcgccca cgcggtcgtc gaagtcgaag gagcagtgta
      181 cacgacgagt catgcgcaac ccgagtctca acccgtcacc gaaggcccgt gcggctgtgg
      241 tcgcactgat tttattactc ggcgctcagc cgacagcgca gacgccgcga gagctgttcg
      301 ccgagtggca gcggcatcgc ttgacgacgc tcgaccccgc gctcgtcaaa caccaagacc
      361 tcaagaagta tctcgccgcg ctcaaggcgc gcggcgtgcc ggtcgcggag gtcgggcggt
      421 ctctggccgg gcgcgagatt tatcggatgg agttcgggcg cggccccgtc aaggtcttca
      481 tgtggtcgca gatgcacggc gacgagccga cggccaccag cacgctcgtc gatctcttcg
      541 cctacctgca cagcaaccgc ggcgtcgcgt gggtgaaggc cgtcgaggag aagctgacgc
      601 tgcgcgcagt gccgatgctc aaccccgacg gcgccgaact ttaccgccgc cgcaacctcc
      661 agtccatcga catcaaccgc gacgcgcgca cgctcacgac gcccgaagga cgcctgctca
      721 aaaagctccg cgacgagtgg cagcccgaca tcggcttcaa cctccacaac cagaacccgc
      781 gcacggcggt cggcgacacg ggcaagcagg cgaccatctc tcttttggcc gtcccctccg
      841 acgcgagcgg cgccgacacg ccggggcgcg tccgcaacaa gcggctctgc gcggtgatgg
      901 tcgaggcgct ggcgccgttc atccgcggcc acgtcgggcg ctacgacgac accttcaacc
      961 cgcgcgcctt cggcgacctc atcagccagt ggggcacgcc cgtcatcctc gtcgagaccg
     1021 gcgcgctcaa aggcacgagc ggccacgacc tcgcgggcct caacttcgtg gcgctctcgg
     1081 cggcgcttcg ctcgctcgcg gacgggagcg ccgaaggcgc gaaccccgcc gtctacgacg
     1141 cgctgccgtt gaacgcgggc gggacgatct acgacctcgt cgtgcgcggc gcgacaatcg
     1201 tcagccgcgc cgcgccgggc ggcgtccgcg tgcagccctt cgcggcggac atcgccgtca
     1261 acgtcgagcg cgggcgcggc gacgccgcgc gcggcggcag gactacggtg caggacgtag
     1321 gcgacctctc cgtctacaag gggctggagg aggttgacgc tcgcgactac tacgtcattg
     1381 ccgccgcggg cgagctgcgt acgggcagcg acgccgcgct cctcttctac cgcaaggccg
     1441 attcgtccgc cgtcaaatgg gacgcgcccg acatcgagaa gcgcttcccg cccgaggccg
     1501 tcttccgcgg cggccagtgg gtgcggcgtg gtaagctgcc ggtcgagtga cgcacggact
     1561 acaatagcgc ttcgcaaatg agtgagaccg tcatgaagac tgtaggggca gggcttgtcc
     1621 ctgcccgccg cgtgcgattg cttcaacgtt gtaaggtact gtgacagcgg gcagggacaa
     1681 gccctgtccc tacaatcaac gcgcacgccg agaggtcttg ccgcggacgc tgatcgatga
     1741 gcaaggaacg gggccgtccc tcatgtctat cgaagactcc atctcgcagc ccgccgagga
     1801 gcgcttccgc gccgtcctcg aacagtcgcc gtacagcatc cagatcatgt cgccggacgg
     1861 gcgcacgctg cgcgtcaacc gcgcgtggga ggagctgtgg ggcgtcaagc tcgaacagat
     1921 cgcgggctac aacatgctcg aagaccggca gctcgtcgag cgcggcatca tgcccctcat
     1981 ccgccgcgcc ttcgagggcg aggccgtcga gctgccgctc atcctctacg acccggaaga
     2041 gaccgtcccc ggcgcgacgc gccaccggga cgcgcgccgc tggacgaagg ccgtcgccta
     2101 cccgatcaag gacgcggcgg ggcgcgtgct cgaagtcgtc ctgatgcacg aggacgttac
     2161 ggcgcgcgtg ctggcggaag aagagaacgc gcggctcgcc gcagaggccg aggcgcggcg
     2221 cacgcggcaa gaggccgacg accgcttcct cgccgaggcg accacggcgc tcgcctcgtc
     2281 gctcgactac gagacgacgc tctcgacgct cgcgcgcctc gccgtcccgc acttcgcaga
     2341 ctggtgcggc gtggacatcg ccgacgaggg cgggacgctg cgccgcctcg ccgtcgcaca
     2401 cgtcgatccg gcgaaggtcg cgtgggcgcg cgagcttcag cagcgctacc cggcagaccc
     2461 ctcggcgccg cgcggctcct acaatgtgct gcgcaccggc gagtcggagt tctaccccga
     2521 catcacggac gagatgcttg tcgccgccgc gcacgacgag gagcacctcg ccgccctgcg
     2581 cgcgatggag ttccgctcgg cgatgatcgt gccgctcaag acgcgcgacc gcgtgctcgg
     2641 catcatcgtg ttcgtcaaca ccgagtccgg gcggcggcac gcgccggctg acctcgcctt
     2701 cgccgaagac ctcgcgggcc gcgcggcgct cgccgtcgag aacgcgcggc tctaccgcgc
     2761 cgagcagcag acgcgcaaag ccgccgagca gacgttcgac cgcctcgcgc ggcttcaggc
     2821 catctcctcg gcgctctcgc aggcgctcac gcagcagcag gtcgccgccg ccgtcatcga
     2881 gcagggcatc agctcgctcg gcgcgcacgc gggcagcgtc gtcgcgctcg acgacgacgg
     2941 cgcgaacctg gacctcgtca ccaccgtcgg cttcccgccg gaggtggtcg aggggtggaa
     3001 gcgcttcgcg ctcgaaacgc ccgtgccgct ggccgacgcg gcgcgcgagc ggctgcccgt
     3061 gctcgtcgaa accttcgcca actattccga gcgctacccg atgctggggc cgatggcttc
     3121 gatcac
//
LOCUS       DBNGOH010000149         2602 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2221240, whole genome shotgun sequence.
ACCESSION   DBNGOH010000149 DBNGOH010000000
VERSION     DBNGOH010000149.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2602)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2602)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2602
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2221240"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..473
                     /locus_tag="AC159P_02710"
     CDS             <1..473
                     /locus_tag="AC159P_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014101178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0061798 - GTP 3',8'-cyclase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA family protein"
                     /protein_id="HIR3694394.1"
                     /translation="MAAFAREHGVSMRFIEYMPLDSGHGWSREQVVSGAEIRQRVNER
                     FLLVSKRVERGSETATRYRFADGTEGEIGIIAPVTEPFCGACSRVRLTADGQIRTCLF
                     SKVEHSLRDVMRSGAARAETADYIASVVMKKEPRHYINETYFEQPARTMSLIGG"
     gene            478..873
                     /locus_tag="AC159P_02715"
     CDS             478..873
                     /locus_tag="AC159P_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017302298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694395.1"
                     /translation="MKVADLSAELFEKIGASRYDESIEKHEGPWAWGSTLDFCEFMVI
                     CGRDVLLPVVRERHANITILRCLPSADGQSLTVFLKDTTFDDDTFSGFVAVCDRFDGQ
                     DFYVAVLYHEWYVVENEWQPRGNQLEFLM"
     gene            1185..>2602
                     /gene="thiC"
                     /locus_tag="AC159P_02720"
     CDS             1185..>2602
                     /gene="thiC"
                     /locus_tag="AC159P_02720"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009961806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HIR3694396.1"
                     /translation="MSTSNNGGERQSGEVQLPNSRRVYAEGPDKALRVPLREIALQPT
                     RLPDGRLETNEPVRVYDTSGPWGDPDCAPRVTDGLPALRREWILARGDVEEYEGRDVV
                     PQDDGYLTEGAAEFAKNKDKGRLEPFPGLRRSPLRARAGACVTQMHYARRGVVTPEME
                     FVALRENMGREAALHAMREHARDDRSSLFHEHRGESHGASLPAFVTPEFVRDEVARGR
                     AIIPANVNHPEAEPMIIGRNFLVKINANIGNSAVASSIEEEVEKMRWATRWGADTVMD
                     LSTGKNIHTTREWILRNSPVPIGTVPIYQALEKVGGRAEELTWEIYRDTLVEQAEQGV
                     DYFTIHAGLRLPYIPLTAKRTTGIVSRGGSIMAKWCLAHHEESFLYTHFGDICEIMRA
                     YDVSFSLGDGLRPGSIADANDEAQFAELDTLGELTKLAWELDCQVMIEGPGHVPMHLI
                     RENMERQLAACHEAPFYTLGPLT"
BASE COUNT          485 a          829 c          850 g          438 t
ORIGIN      
        1 acatggccgc cttcgcgcgc gagcacggcg tttcgatgcg cttcatcgag tacatgccgc
       61 tcgactcggg ccacggctgg tcgcgcgagc aggtggtctc cggcgccgag attcgccagc
      121 gcgtcaacga gcgcttcctg ctcgtctcca agcgcgtcga gcgcggctcc gagacggcca
      181 cgcgctaccg cttcgccgac ggcaccgagg gcgagatcgg catcatcgcg cccgtcaccg
      241 aacccttctg cggcgcctgc tcgcgcgtgc ggctcaccgc cgacggtcag atcaggacgt
      301 gcctcttctc gaaggtcgag cactctctgc gcgacgtgat gcgctccggc gccgcgcgcg
      361 ccgagacggc agactacatc gcgagcgtcg tcatgaagaa ggagccgcgc cactacatca
      421 acgagaccta cttcgaacag cccgcccgca cgatgagcct catcggggga tagaatcatg
      481 aaggtagccg acctttcagc cgagctgttc gaaaagatag gggccagccg ctacgacgaa
      541 agcatcgaaa agcacgaagg cccgtgggct tggggttcga ctctcgactt ctgcgagttc
      601 atggtcatct gcgggcgcga cgttcttttg cccgtcgtcc gcgagcggca cgcaaacatc
      661 acgattctgc gctgtctacc gagcgccgac ggccagtcct tgacggtctt cctcaaggac
      721 accacattcg acgatgatac tttttccggc ttcgtcgccg tctgcgacag attcgacggg
      781 caagactttt acgtggcagt cctttaccat gagtggtacg tcgtggaaaa cgagtggcag
      841 cctcgtggta atcagcttga gtttctgatg tagtgaaaaa gtttttaacg ggggagccgc
      901 gctactttgt gtgtgacgcg gctgagagtc cgcaaattgc ggattgcgga ttgcggattg
      961 cggattgaag aagggcgatg atcgacgaca ccggtgctgt catcaatcgt ctcttccgat
     1021 cagcaatccg taatccgcaa tcggcaatcg aggagaccct ttgaacctga tgcggttagt
     1081 accgccgaag ggaggcttga ggggaaccgt cccggccttc gtgcgcgggg cggttcttcc
     1141 ttttcgggcc ggacgggaag gcccagccga aaggacgaag agcaatgagc acatcgaata
     1201 acggcggcga gcggcagagc ggcgaggtgc aactgccgaa ctcgcgcagg gtctacgccg
     1261 aagggccgga caaagccttg cgcgtccctt tgcgcgagat cgccttgcag ccgacgcggc
     1321 tgcccgacgg gcgtctggaa acgaacgagc cggtgcgcgt ctacgacact tcggggccgt
     1381 ggggcgaccc cgattgcgcg ccgcgcgtga cggacgggct gccggccttg cgccgcgagt
     1441 ggattctcgc gcgcggcgac gtggaagagt acgaggggcg cgacgttgtc ccgcaggacg
     1501 acggctacct gacggagggc gcggcggagt tcgcgaagaa caaggacaag ggccggctcg
     1561 aaccgttccc cggcctcagg cgctcgccgc tgcgcgccag ggcgggcgcg tgcgtcacgc
     1621 agatgcacta cgcgcggcgc ggcgtcgtca cgcccgagat ggagttcgtc gcgctgcgcg
     1681 agaacatggg gcgcgaggcg gcgctgcacg cgatgcgcga acacgcgcgc gacgatagaa
     1741 gctcgctctt tcacgagcac cgcggcgaat cccacggcgc ttcgctcccc gcgttcgtga
     1801 cgcccgagtt cgtgcgcgac gaggtggcgc gggggcgcgc catcatcccc gccaacgtca
     1861 accaccccga agccgagccg atgattatcg ggcggaactt cctcgtcaag atcaacgcca
     1921 acatcggcaa ctcggcggtc gcctcctcga ttgaagaaga ggtcgagaag atgcgctggg
     1981 cgacgcgctg gggcgcagac accgtgatgg acttgtccac cggcaagaac atccacacca
     2041 cgcgcgagtg gattctccgc aactcgcccg tccctatcgg caccgtgccg atctatcagg
     2101 cgctcgaaaa agtcggcgga cgcgccgaag agctgacgtg ggaaatctac cgcgacacgc
     2161 tggtcgagca ggccgagcag ggcgtcgatt acttcaccat ccacgcgggt ctgcggctgc
     2221 cttacatccc tctgacggcg aagcgcacga ccggcatcgt ctcgcgcggc ggctcgatca
     2281 tggccaagtg gtgtctggct caccacgagg agagcttcct ctacacacat tttggcgaca
     2341 tctgcgagat catgcgcgcc tacgacgtgt cgttctctct gggcgacggc ctgcgccccg
     2401 gctccatcgc cgacgcgaac gacgaggcgc agttcgccga actcgacacg ctcggcgagc
     2461 tgacgaagct cgcttgggaa cttgactgtc aggtgatgat cgaggggccg ggccacgtgc
     2521 cgatgcacct catcagggag aacatggagc ggcaactggc ggcctgtcac gaggccccgt
     2581 tctacacgct cgggcctttg ac
//
LOCUS       DBNGOH010000150         2524 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3321192, whole genome shotgun sequence.
ACCESSION   DBNGOH010000150 DBNGOH010000000
VERSION     DBNGOH010000150.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2524)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2524)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2524
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3321192"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            116..1621
                     /gene="gltX"
                     /locus_tag="AC159P_02725"
     CDS             116..1621
                     /gene="gltX"
                     /locus_tag="AC159P_02725"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HIR3694397.1"
                     /translation="MTVRVRFAPSPTGYLHIGAARTALFNWLFARKSGGKFLLRVEDT
                     DLERSTEGSTRSIIEGLEWLGISDYDEEIVFQSANAGKHREASLRLVREGKAYRDFTP
                     KEARADGDVKQEIAERARGRAAAEGVDHRSNPYRDLPLAESEQRAAAGEAFAVRLKVP
                     REGRSRFVDAVYGEQERDYTEIEDLVLLRSGDLSPLYNLSVVVDDIEMRITHVVRGQD
                     HLTNTHKQILIYRALGSDVPTFAHLPLILAPNKQKLSKRKHGQVVSLTTYRDSGFVPA
                     AFRNYLALLGWSPGEENKELLSLEEMVEQFSLEGIHRSNAVFNFSESDPRQWTDQKAL
                     WMNAEYIRTMTPEELLPMVQGELEAAGLWRAEYAGEERAWFERTVDLVRQRFYTLKDF
                     SGQGRAYFADDFEFDDAAVKKNLRKEPRLRELLPALAERLEGVEPWTVESIEKALRAY
                     ADEAGVKAGLFINASRTALTGQAVGPSMFDVFEAIGRERSAKRLREAARRV"
     gene            1709..2434
                     /locus_tag="AC159P_02730"
     CDS             1709..2434
                     /locus_tag="AC159P_02730"
                     /inference="COORDINATES: protein motif:HMM:TIGR02246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YybH family protein"
                     /protein_id="HIR3694398.1"
                     /translation="MIRQTLFAAALVFAFAAPALAQNAASSTPRPAAATRPRTSAPKT
                     NATPAAAAAGEAQQDTTPAPAPPPTSTTGTGAARPALRRQSAADVAQTRAVRAAFDTL
                     IDGIRRADAKAVMDVYWNSPQLVLFNNNGTVTKTWEQVRQNRESLYAKVSDVKLDVRD
                     VQVRLLGPSAAVVSCLWEQSQTADGQPERATGRLSLVFQKVGTAWKAVHAHTSPDRPD
                     PSRLLPSERTTTTTPAEPPAAKP"
BASE COUNT          421 a          849 c          867 g          387 t
ORIGIN      
        1 gagtgacaag tctttagagc ttgtccgtct cgtcggctgt cactcgtcgc cgttttagcg
       61 tgatgattag agatcataac tcgtcacggt tgacggttca cggttcacgg ctcttatgac
      121 tgtccgcgtt cgtttcgcac catcgcccac gggctacctg cacatcgggg cggcgcgcac
      181 cgcgctgttc aactggctgt tcgcgcgaaa gtcgggcggc aagttcctgc tgcgcgtcga
      241 ggacacggac cttgagcgct cgaccgaagg gtccacgcgc tcgatcatcg aggggctgga
      301 gtggctcggc atcagcgact acgacgagga gatcgtcttc cagtccgcca acgccgggaa
      361 gcaccgcgag gcgtcgctgc ggctcgtcag ggaaggcaag gcgtaccgcg acttcacgcc
      421 gaaggaagcg cgcgccgacg gcgacgtgaa gcaggagatc gccgagcgtg cgcgcgggcg
      481 cgcggcggcg gaaggcgtcg atcaccgcag caacccgtac cgggatttgc cgctcgcgga
      541 gtcggagcaa agagcggcgg cgggcgaagc gttcgcggtg cgtttgaagg tgccgcgcga
      601 ggggcgctcg cggttcgtgg acgcggtgta cggcgagcag gagcgcgact acacggagat
      661 cgaagacctc gtgctgctgc gctcgggcga cctctcgccg ctctataacc tgtcggtcgt
      721 cgtggacgac atcgagatgc gcatcacgca cgtcgtccgc gggcaggatc acctgacgaa
      781 cacgcacaag cagattctca tctaccgggc gctcggctcc gacgtgccga cgttcgcgca
      841 cctgccgctc atcctcgcgc cgaacaagca gaagctctcg aagcgcaagc acgggcaggt
      901 cgtttccttg acgacctacc gcgacagcgg cttcgtgccc gccgcgttcc gcaactacct
      961 ggcgctcctg ggctggtcgc cgggcgagga gaacaaggag ctgttgtcgc tcgaggagat
     1021 ggtcgagcag ttctcgctcg aaggcatcca ccgctcgaac gccgtcttca acttcagcga
     1081 gtctgacccg cgccagtgga cggatcagaa ggcgctctgg atgaacgccg agtacatccg
     1141 cacgatgact ccggaagaac tgctgccgat ggtgcagggc gagcttgagg cggcggggct
     1201 gtggcgggcc gagtacgccg gagaggagcg cgcgtggttc gagcgcaccg ttgacctcgt
     1261 ccgccagcgc ttctacacgc tcaaggattt ctccgggcag ggccgcgcct acttcgccga
     1321 cgacttcgag ttcgacgacg cggcggtgaa gaagaacttg cggaaggagc cgcgtttgcg
     1381 agagctgctg cccgcgctcg ccgaacggct ggaaggcgtg gagccgtgga cggtcgaatc
     1441 gatcgagaag gctttgcgcg cctacgcgga cgaggcgggc gtgaaggccg gcctcttcat
     1501 caacgcctcg cgcacggcgc tcacgggtca ggcggtcggc ccctccatgt tcgacgtgtt
     1561 cgaagcgatc gggcgcgagc gctcggcgaa gcggctgcgc gaagcggcac ggcgcgtctg
     1621 aattttttcg tcacctcgcg gcgctcggcc cgcggctcca gccgtcggcg ataagcgccg
     1681 cgtctttttt ttggagaaag gaattatcat gattcgtcag acactcttcg cggcggcgct
     1741 cgtcttcgcc ttcgccgccc cggccctcgc gcagaacgcc gcttcgagca cgccgcgacc
     1801 ggcggcggcg acgcgcccgc gcacctccgc gccgaagacc aacgcgacgc ccgccgccgc
     1861 cgccgcggga gaagcgcagc aggacacgac ccccgctccc gcgcctccgc cgacgagcac
     1921 gaccggcacc ggcgcggcgc gtcctgcgct gcgcaggcag tccgccgcgg atgtagcgca
     1981 gacgcgcgcc gtccgcgccg ccttcgacac gctcatcgac ggcatccgtc gcgccgacgc
     2041 caaggccgtg atggacgtgt actggaactc gccgcagctc gtcctcttca acaacaacgg
     2101 gacggtgacg aagacttggg agcaggtgcg ccagaaccgc gagagcctct acgcgaaggt
     2161 ctcggacgtg aagctcgacg tgcgcgacgt gcaggtgcgc ctgctcgggc cgtcggcggc
     2221 ggtcgtctcg tgcctgtggg agcagtcgca gaccgcggac ggtcagcccg agcgcgcgac
     2281 cgggcgcctg tcgctagtct tccagaaggt cggcaccgcc tggaaggccg tccacgcgca
     2341 cacctcgccg gacaggcccg acccttcgcg cctgctgccc tccgagcgca cgacgacgac
     2401 cacgcccgcc gagccgccag ccgcgaagcc ataagacggc cactcgactc gcccgacgga
     2461 cagcgaacgg gagtggtgcg gttgtgtgga gcgggcgctc atttcagcgc ggagtgcgcc
     2521 gcag
//
LOCUS       DBNGOH010000151         6496 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1488201, whole genome shotgun sequence.
ACCESSION   DBNGOH010000151 DBNGOH010000000
VERSION     DBNGOH010000151.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6496)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6496)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6496
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1488201"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            142..714
                     /locus_tag="AC159P_02735"
     CDS             142..714
                     /locus_tag="AC159P_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HIR3694399.1"
                     /translation="MSTTRLITADELLTMPHYEGRNDIRLALIRGELKKMSPTGGTHG
                     ILCATIGAELIIFVREKDLGLVFGAETGFIVEREPDTVVGADVAFVSHERMKDVENLD
                     KFLPFAPDLAVEVLSPGNTVQEIDEKIARYFAAGSRAVWVFKPKQRAVAVYGSPFDVR
                     ILGEQDTLDGGEVLPGFTLDLSKLFAAVKK"
     gene            739..5181
                     /locus_tag="AC159P_02740"
     CDS             739..5181
                     /locus_tag="AC159P_02740"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR3694400.1"
                     /translation="MKLSPTEVFQDRNIFIIGATGFLGKVTLSMLLHRFPGVGKVYVT
                     VRARSREESETRFWNNVITAPPFDPLRERYGAAFEDFIRDKVVLVNGDIAEDNLGFSE
                     EEAQRVAEDIDVLVNSAGNVTFNPTLESALRTNVVGTQNVISFARRMKRPALVHVSTC
                     FVAGNRSGPVWESDPVIGYFPRREELPGVEFSVEQEIRDCAKLAERVREEARDAMQAA
                     RFRENARKRLIEEGRDPDDLDAMGLAVARERKIWTRERLTELGVERAAFWGWPNIYTY
                     TKSLGEQLVAAEKGMVCSIVRPSIVESAIKYPFPGWNEGFTTTAPLIFITFKGQTVIP
                     ANNKLILDITPVDQVAAVMLAVAAEACVGEPRLVYQAATGDSNPNNMERIVGLVGLYK
                     RKHFQEKETGFKLLNELAGRMEAKTVSPSRFDATSTPMIHAAAKKLSGALDHVRPRWG
                     GGRLTDIVDQAKATMDRVEEVTRETKEAFELFRPFTVENSYVFRADNVRSLFARIRED
                     EQALLRWNPEQFDWYDYWMNVHFPGLKKWVFPTLEEDMRAQPKRVYTYRDLVELFETS
                     TKRFSTRVAMRIERDGQKEQYTYADLHELATRAAAFFAGHGVKPGDRVLLASQNAPEW
                     GISYFGVLKAGATCVPVDHESTTEELVNFVRASGAVGVVVSPKVDEEHADLNEALGAA
                     GLEGTRIWRFVEVFELTDQQTEDERVALLPPRVQAQTVASLIFTSGTTGQPKGVMLSH
                     KNLSNMVSMLSSVFEMDTGDGVLSVLPLHHTFEFSTGFLTPFSRGTQITYLPELNGEE
                     LARAIKNGHVTGMVGVPALWELLHRRITNRLNENGKWVGQAAELLMRANSWLRDRTPL
                     NLGQVVFRPIHAGMGGRIRYLISGGSALDEKIKRDMHGLGFTILEGYGLTEASPVLTV
                     TRPQNKLIDASVGKPLPGVEIKIAEPDAAGIGEVIARGANVMLGYYRNEGATQAALVN
                     RWLHTGDLGRMDDDGNLYLVGRSKEIIVDSNGKNVYPDEIEELYKDSPFVKELGVVGL
                     PDQQGERVACLVVPDYGNDISLSRDETRRKIEQHFRDVSSALPFFKRVKALYFTDDEL
                     PRTATRKVKRREVVEIIERMEALAREGLKESTSARDAGGGETAWLVDLLAHVSNRPRS
                     QISLDTRMSELGFDSLMFVELATAIEQAGGTLLTPDTLNEVQDVRELQTVVKRRATPA
                     QKRAAEARAEEARRHEDEEIYIPSLVRTLGNRGLDVVQQTFYERFLKADIEGQSNIPA
                     HTNFIAASNHASHLDIGLVKVALGEYGRDMVALAAADYFFDNKYKRAYMNNFTNLVPI
                     ERSGSLRQSLRHARSFLERGHTALVFPEGTRSMTGEMAEFKSGFAYLALSTRTGILPI
                     YIHGTYEAFPKGSTVLKGREVGARIGRFLPAEELEDLTRGMPKSEAYRLVAALVRHEI
                     ENLRDGTRHAFDSAALRRRWKAERRANNHHEDAHDAGEMATTGD"
     gene            5294..6397
                     /locus_tag="AC159P_02745"
     CDS             5294..6397
                     /locus_tag="AC159P_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011985525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR3694401.1"
                     /translation="MATTKQTKTKSAAKAAKAVAPRRTALAKRTPPLTLVTGGTGFLG
                     THLVRLLLDEGVKNVRVMAGSAPPWAEESGVEVVKGSITSPADVARAVEGAAEVYHLA
                     GRVSRAKEDAHLMYSVHVEGTRLLCEAARAAGVKSLVLASTSGTVAVAETGDAVPDED
                     WPAPVDIISRWPYYASKLYQERVAREHFAGKGLRLVTVNPSLLLGPGDERLSSTKVVL
                     DFLARKIMTVPSGGLSFVDARDAARAFYSAMQCGKDGERYLLGAANWTFDEFFGRLER
                     LTKISAPRVKLPSKLALVGARGLNALYTRWKLAPPVEPTEVEMADYFWYVDARKAQRE
                     LGFAPRDPQETLLDTVNYVRANFLGSGAFKNAG"
BASE COUNT         1206 a         2143 c         2131 g         1016 t
ORIGIN      
        1 cggctgggat cgcgccgaca acctgaccga ggccatcgcc caggcccgct ctatcgtcgg
       61 gcgctccgcc gagattacaa tgctccacca gccggtgatc gccatctgcg acatgagtta
      121 aggcgcgaac gaggtgaaga gatgagcacg acgcggttga taacggcgga cgagttactg
      181 accatgcccc actacgaggg gcgcaacgat attcgcctgg cacttatcag aggagagttg
      241 aaaaaaatgt ctcccacagg cggcacccac ggcatcctct gcgcaacgat aggcgccgaa
      301 ctgataatct tcgtgcggga aaaagacttg ggcttagttt tcggggcgga gacagggttc
      361 atcgttgagc gtgaacccga caccgtggtc ggcgccgatg tcgctttcgt gagccacgaa
      421 cggatgaagg atgtcgagaa cctcgataag tttctgccgt tcgcgcccga tttggccgtc
      481 gaagttctca gccccggcaa cactgttcag gagattgacg agaagattgc tcggtacttc
      541 gccgccgggt cgcgcgcagt ctgggttttt aagccgaagc agcgcgccgt ggcggtttac
      601 ggctcgccgt ttgacgtgcg catcctcggc gagcaggata cgctcgacgg cggcgaggtg
      661 ctgccggggt tcacgctcga tttgtcgaag ctgttcgccg ccgtcaaaaa gtgagtggca
      721 gtcatcgcag acgaaacaat gaagctatct ccgacagagg ttttccaaga ccgcaacatc
      781 ttcatcatcg gggccacggg tttcctcggc aaggtgacgc tctcgatgct gctgcaccgg
      841 ttccccggcg tggggaaggt gtacgtgacg gtgcgcgcgc ggtcgcgcga ggagtcggag
      901 acgaggttct ggaacaacgt catcacggcg ccgccgttcg accccctgcg cgagcggtac
      961 ggcgcggcgt tcgaggactt catccgcgac aaggtcgtgc ttgtcaacgg cgacatcgcg
     1021 gaggacaatc tggggttcag cgaagaagaa gcgcagcggg tcgccgaaga catcgacgtg
     1081 ctcgtcaaca gcgcgggcaa cgtcaccttc aacccgactt tggagagcgc gctccgcacg
     1141 aacgtcgtcg gcacgcagaa cgtgatctcg ttcgcgcgca ggatgaagcg cccggcgctg
     1201 gtgcacgtct cgacgtgctt cgtggcgggc aaccgctcgg ggccggtgtg ggagagcgac
     1261 cccgtgatcg gctacttccc gcgccgcgaa gagctgccgg gcgtcgagtt ctcggtcgag
     1321 caggagatac gcgactgcgc aaagctcgcc gagcgcgtgc gcgaagaggc ccgcgacgcg
     1381 atgcaggccg cgcgcttccg cgagaacgcg cgcaagcgcc tgatcgaaga ggggcgcgac
     1441 ccggacgatc tggacgcgat gggactggcg gtcgcgcgcg agcgcaaaat ctggacgcgc
     1501 gagcggctga ccgaactcgg tgtcgagcgc gcggccttct ggggctggcc gaacatctac
     1561 acctacacga agagcctcgg cgaacagctc gttgccgccg agaaggggat ggtctgctcc
     1621 atcgtccgcc cgtccatcgt cgagtcggcc atcaagtatc cgttccccgg ctggaacgag
     1681 gggttcacga cgacggcgcc gctcatcttc atcaccttca agggccagac cgtcatcccc
     1741 gccaacaaca agctcatcct cgacatcacg cccgtcgatc aggtcgcggc ggtgatgctg
     1801 gcggtcgcgg cggaagcgtg cgtcggcgag ccgcggctcg tctatcaggc cgcgaccggc
     1861 gacagcaacc cgaacaacat ggagcgcatc gtcgggctgg tcggcctcta caagcgcaaa
     1921 cattttcagg agaaggagac gggcttcaag ctcctgaacg agctggcggg ccggatggag
     1981 gcgaagaccg tcagcccctc gcgcttcgac gcgacctcga cgccgatgat ccacgcggcg
     2041 gcgaagaagc tgtcgggcgc gctcgaccac gtgcgcccgc gctggggcgg cggtcgcctg
     2101 acggacatcg tcgatcaggc gaaggcgacg atggatcgcg tcgaagaggt gacgcgcgag
     2161 accaaagagg cgttcgagct gttccgcccg ttcacggtcg agaactctta cgtcttccgc
     2221 gccgacaacg tgcgctcgct cttcgcgcgc atccgcgagg acgagcaggc gctcctgcgc
     2281 tggaaccccg agcagttcga ctggtacgac tactggatga acgtccactt ccccggcctc
     2341 aagaagtggg tcttcccgac gctcgaagag gacatgcgcg cgcagcccaa gcgcgtctac
     2401 acctaccgcg acctcgtcga gcttttcgag acctcgacca agcgcttctc gacgcgcgtc
     2461 gccatgcgca tcgagcgcga cgggcaaaaa gaacagtaca cctacgcaga cctgcacgag
     2521 ctggcgacgc gcgccgccgc cttcttcgcc ggccacggcg tgaagcccgg cgaccgcgtg
     2581 ctgctcgcca gccagaacgc gcccgagtgg ggcatcagct acttcggcgt gctgaaggcg
     2641 ggcgcgacgt gcgtccccgt agaccacgag agcacgaccg aagaactcgt caacttcgtc
     2701 cgcgcgagcg gcgcggtcgg cgtcgtcgtc agcccgaagg ttgatgaaga gcacgctgat
     2761 ttgaatgaag ctctcggcgc ggcgggtctc gaagggacgc gcatctggcg cttcgtggag
     2821 gtcttcgagc tgaccgacca gcagaccgag gacgagcgcg tcgcgctgct gccgccgcgc
     2881 gtgcaggcgc agaccgtcgc ctcgctcatc ttcacctcgg gcacgacggg ccagcccaaa
     2941 ggcgtcatgc tctcgcacaa gaatctgtcg aacatggtct cgatgctctc gtccgtgttc
     3001 gagatggaca cgggcgacgg cgtgctctcg gtcttgccgc tccaccacac gttcgagttc
     3061 tcgaccggct tcctcacgcc gttcagccgc ggcacgcaga tcacttacct gcccgaactc
     3121 aacggcgaag aactcgcgcg cgccatcaag aacggccacg tcacaggcat ggtcggcgtc
     3181 cccgccttgt gggagctgct gcaccgccgc atcacgaacc gtctgaacga aaacggcaag
     3241 tgggtgggcc aagccgcaga gctgttgatg cgcgccaaca gttggctgcg cgaccgcacg
     3301 ccgctgaacc tcgggcaggt cgtcttccgc cccatccacg ccgggatggg cgggcgcatc
     3361 cggtatctta tctcgggcgg ctcggcgctc gacgagaaga tcaagcgcga catgcacggc
     3421 ctcggcttca ccatcctcga gggctacggg ctgacggaag cctcgcccgt gctgaccgtg
     3481 acgaggccgc agaacaagct gatcgacgcg agcgtcggga agcccttgcc cggcgtcgag
     3541 attaaaatcg cggagccgga cgccgcaggc atcggcgagg ttatcgcgcg cggcgccaac
     3601 gtgatgctcg gctactaccg caacgagggc gcgacgcagg ccgcgctggt caaccgctgg
     3661 ctccacacgg gcgacctcgg gcgcatggac gacgacggca acctctacct cgtcggtcgc
     3721 tcgaaggaga tcatcgtcga ttcgaacggc aagaacgtct acccggacga gatcgaagag
     3781 ctgtacaagg actcgccctt cgtcaaagaa ctcggcgtgg tcggcctccc cgaccagcag
     3841 ggcgaaaggg ttgcgtgcct cgtcgtcccc gactacggca acgacatcag cctctcgcgc
     3901 gacgagacgc gccgcaagat cgagcagcac ttccgcgacg tgtcctcggc gcttcccttc
     3961 ttcaagcgcg tcaaggcgct ctacttcacg gacgacgagc tgccgcgcac cgcgacgcgc
     4021 aaggtcaagc gccgcgaggt ggtcgagatc atcgagcgga tggaggcgct ggcgcgcgag
     4081 gggctgaagg agagcacctc ggcgcgcgac gcggggggcg gcgagaccgc ttggctcgtc
     4141 gatctcctgg cgcacgtctc gaaccgcccg cgctcgcaaa tctcgctcga cacgcggatg
     4201 agcgaactcg gcttcgacag tctcatgttc gtcgagctgg cgacggccat cgagcaggcg
     4261 ggcggcacgt tgctgacgcc cgacaccttg aacgaggtgc aggacgtgcg cgagcttcag
     4321 accgtggtga agcgccgcgc gacgcccgcg cagaagcgcg ccgccgaggc ccgcgccgaa
     4381 gaagcgcggc ggcacgagga cgaagagata tacatcccct cgctcgtccg cacgctcggc
     4441 aaccgcggcc tcgacgtggt gcagcaaacc ttctacgaac gcttcctgaa ggccgacatc
     4501 gagggccagt cgaacatccc cgcgcacacg aacttcatcg ccgcgtcgaa ccacgcctcg
     4561 cacctcgaca tcgggctggt caaggtcgcg ctcggcgagt acgggcgcga catggtcgcg
     4621 ctggccgccg ccgactactt cttcgacaac aaatacaagc gcgcctacat gaacaacttc
     4681 acgaacctcg tccccatcga gcgttcgggg agtttgagac agtcgctccg ccacgcgcgt
     4741 tcgttccttg agcgcggcca cacggcgctc gtcttccccg aaggcacgcg ctcgatgacg
     4801 ggcgagatgg ccgagttcaa atccggcttc gcctacctcg cgctctcgac gcgcaccggc
     4861 atcctgccca tctacatcca cggcacctac gaggcgttcc ccaaaggctc gaccgtgctc
     4921 aagggccgcg aggtcggcgc gcgcatcggt cgcttcctcc ccgccgaaga actcgaagac
     4981 ctaacgcgcg ggatgcctaa atcggaagcc taccgcctcg tcgccgccct cgtccgccac
     5041 gagatcgaga acctgcgcga cggcacgcgc cacgccttcg actctgccgc cctgcgccgc
     5101 cgctggaagg ccgagcgccg cgccaacaat caccacgaag acgcgcacga cgcgggcgag
     5161 atggcgacca ccggagactg aaggacaacg atgaatgcgg aatgatgaat gatgaatgag
     5221 aagcacgtta cctttcatcg ttcaaggtgc ctcgtattca tcattcatca ttccgcattc
     5281 atcatttctc atcatggcga cgacgaagca aaccaagacc aagtctgcgg cgaaggccgc
     5341 gaaggcggtc gcgccgagac ggacggcgct ggcgaagcgc acaccgccgt tgacgctggt
     5401 gacgggcggg acgggctttc tggggacgca cctcgtgcgg ctgctgttgg acgagggcgt
     5461 gaagaacgtg cgcgtgatgg ctggttccgc gccgccgtgg gccgaagagt cgggcgtgga
     5521 ggtcgtgaag ggttcgatca cttcgcccgc ggatgtcgcg cgcgcggtcg agggcgcggc
     5581 ggaggtctat cacctcgcgg gccgcgtctc gcgcgcgaag gaggacgcgc acctgatgta
     5641 ctcggtgcac gtggaaggca cccggctgct ctgcgaggcg gcgcgcgcgg cgggcgtcaa
     5701 gtcgctcgtg ctggcctcga cgagcggcac cgtcgccgtc gccgagacgg gcgacgccgt
     5761 gcccgacgag gactggcccg cacccgtgga catcatctcg cgctggccct actacgcgag
     5821 caagctctat caggagcgcg tcgcgcgcga gcacttcgcg gggaagggcc tgcgcctcgt
     5881 caccgtgaac ccgagcctgc tgctcggccc cggcgacgag cgcctgagtt cgacgaaggt
     5941 cgtgctcgac ttcctcgcgc gcaagatcat gaccgtgcct tcgggcggcc tcagcttcgt
     6001 ggacgcgcgc gatgccgcgc gggcgttcta ctcggcgatg cagtgcggca aggacggcga
     6061 gcgctacctg ctcggcgcgg ccaactggac gttcgacgaa ttcttcgggc ggctggaacg
     6121 gctgacgaag atcagcgccc ctcgcgtcaa gctcccctcg aagctcgcgc tggtgggcgc
     6181 gcgcggcctg aacgcgctct acacgcgctg gaagctcgcc ccgcccgtcg agccgaccga
     6241 ggtcgagatg gcggactact tctggtacgt ggacgcacgc aaagcccaac gcgaactcgg
     6301 cttcgcgccg cgcgacccgc aggagacctt gctcgatacg gtcaactacg tgcgcgcgaa
     6361 tttcctcggc agcggcgcct tcaagaatgc cgggtgagtt ttcgtccgct ggttgagtta
     6421 agcgatagtc cgtctcaacg cagaggacgc ggaggggcgc agagggcgca gagagaaaga
     6481 cattgcttct tctctg
//
LOCUS       DBNGOH010000152         2898 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1855649, whole genome shotgun sequence.
ACCESSION   DBNGOH010000152 DBNGOH010000000
VERSION     DBNGOH010000152.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2898)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2898)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2898
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1855649"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            94..786
                     /gene="cmk"
                     /locus_tag="AC159P_02750"
     CDS             94..786
                     /gene="cmk"
                     /locus_tag="AC159P_02750"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020787599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0036431 - dCMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HIR3694402.1"
                     /translation="MTKSFIIAIDGPSGAGKSTLGRRLARELGLLYIDTGAMYRAVAL
                     AVTRSGVSTTDAGAVAEVARSAQIRLEGDPDSLRVWLDGQDVSEEIRGEQVGHTASIV
                     SAIAEVRRELVGQQRRLGERAGGAVLDGRDIGTVVFPAADVKFFLTAVPEERARRRFE
                     ELHAHDQTFEETLADITARDRRDSTREDSPLAIAADAVVIDTTELSVEDVFRRMTEIV
                     RERRAAAGGTPT"
     gene            949..1608
                     /locus_tag="AC159P_02755"
     CDS             949..1608
                     /locus_tag="AC159P_02755"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HIR3694403.1"
                     /translation="MFTGIIEELGRVRSVERRGEGVRIRFEARVVTEGTHEGDSVSVN
                     GVCLTALDVRPDSFAADGSRETLQRSTLGTLRAGLPVNLERAVTPSTRLGGHIVQGHV
                     DARGRFLSAEDHGGSWTVRIAYPPDLARYLVFKGSVAVEGNSLTVAALAPEHFEIAVI
                     PKTWTLTNLSTLRPGDPVNLEADIIAKYVERILAVGGKAQETDSSSSLTTEKLADLGY
                     P"
     gene            1689..2816
                     /locus_tag="AC159P_02760"
     CDS             1689..2816
                     /locus_tag="AC159P_02760"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF021466.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate phosphatase App1 family protein"
                     /protein_id="HIR3694404.1"
                     /translation="MAKQLTAYPAYGYRDKTDASYWVVPMRVWVFKPRDFIVDDLAVE
                     LFERKLEAEFTPAEREVLKGRIANFIADDDSFERVEFRFEGDDEAHRFEGRTDSNGLV
                     EQTFRFPAAKLEALRDARGWLTYVARSDDEEARGRVRLLEPEGVSVVSDIDDTIKVTN
                     VPAGTETVLRRSFLMDFEAASYTFKAEGKEAAKRQMRELYWELHAEHEAADVAFHYVS
                     GSPWQLFRLLDDFLVRRREGFPEGTFHMKSLRKNLTDPDTWADLFGLAQGKKATLEQK
                     IKQITELMINLPRRRFILIGDSGEMDPEVFLAMNEMFPNHVARIIIRDVVGERLKHPK
                     IEVLPGDTVRYDTRKVLKKIIREMALDIKIEQAAEPPQTEG"
BASE COUNT          525 a         1016 c          916 g          441 t
ORIGIN      
        1 gctgaggact gaggactgag taggaacagc gttcctctca tcaacagtca aagcttttac
       61 tcagtcctca gtcctcagcc ctcagtacta cttatgacga agtccttcat catcgccatc
      121 gacggccctt cgggcgcggg caaatcgacg ctgggccgga ggctcgcgcg cgagctgggg
      181 ctgctctaca tcgacacggg cgcgatgtac cgcgcggtgg cgctcgccgt aacgcgctcg
      241 ggcgtgagca cgacggacgc cggggcagtc gcggaagtcg cgcggagcgc gcagatccgc
      301 ctcgaaggcg accccgactc tctgcgcgtg tggctcgacg ggcaagacgt gtccgaagag
      361 attcgcggcg agcaggtggg ccacaccgcc tcgatcgtct cggccatcgc cgaggtgcgg
      421 cgcgagctgg tcgggcagca gcgccgtctg ggcgagcgcg cgggcggcgc cgtcctcgac
      481 gggcgcgaca tcggcaccgt cgtcttcccc gcggcggacg tgaagttctt tctcaccgcc
      541 gtcccagagg agcgcgcccg ccgccgcttc gaagagctgc acgcgcacga ccagaccttc
      601 gaggagacgc tcgccgacat caccgcccgc gaccgccgcg actcgacgcg cgaagactcg
      661 cccctcgcca tcgccgccga cgccgtcgtc atcgacacca cggagctttc cgtcgaggac
      721 gtgttccgcc gcatgaccga gatcgtccgc gagcgccgtg cggcggcggg cgggactccg
      781 acttgaagca ggccgcatcg aagaagtatc gaaggagccg ttgaccatga acacgcgctt
      841 ttcattcaaa gccgaggcga agtggattct gctgttcagc ctcgcgcccg cgctcctcgg
      901 cctcgccgcc gtccttatcg tcggtctctt ccgctgactc tcgaacctat gttcaccggc
      961 atcatcgaag aactcgggcg cgtgcgctcc gtcgagcggc gcggcgaggg cgtgcgcatc
     1021 cgcttcgagg cgcgcgtcgt caccgaaggg acgcacgagg gcgactccgt ctccgtcaac
     1081 ggcgtctgcc tcaccgcgct cgacgtgcgg cccgactcgt tcgcggcgga cggctcgcgc
     1141 gagaccttgc agcgctcgac gctcggcacc ctgcgcgcgg gcttgcccgt caacctcgaa
     1201 cgcgccgtca cgccctcgac ccgcctcggc ggccacatcg tgcagggcca cgtggacgcg
     1261 cgcgggcgct tcctctcggc ggaagaccac ggcggctcgt ggacggtgcg catcgcctac
     1321 ccgcccgacc tcgcccgcta cctcgtcttc aaaggctcgg tcgccgtcga aggcaacagc
     1381 ctcaccgtcg ccgccctcgc ccccgaacac ttcgagatcg ccgtcatccc caagacctgg
     1441 acgctcacca acctctccac cctgcgcccc ggcgaccccg tcaacctcga agccgacatc
     1501 atcgccaaat acgtcgagcg catcctcgcc gtcggcggga aggcgcagga gaccgattct
     1561 tcatcgtcgc tcacgacgga gaagctggcc gacctgggct atccgtgaac ccgttagcga
     1621 acgcgtctga aacacttccg tcggcccccg catatatcaa cgggaagagt tataacggag
     1681 gctccgagat ggcaaaacaa ttgacggcgt acccggcata cggataccgc gacaagacgg
     1741 acgcgagcta ctgggtcgtg ccgatgcgcg tgtgggtgtt caagccgcgc gacttcatcg
     1801 tggacgatct ggcggtggag ctgttcgagc gcaagctcga agcggagttc acgcccgccg
     1861 agcgcgaagt gctgaagggc cgcatcgcca acttcatcgc cgacgacgac tcgttcgagc
     1921 gcgtcgaatt ccggttcgag ggcgacgacg aggcgcaccg tttcgagggg cggacggaca
     1981 gcaacggtct ggtcgagcag accttccgct tcccggcggc caagctcgaa gctttgcgcg
     2041 acgcgcgcgg gtggctgacc tacgtcgcgc gctcggacga tgaagaggcg cgcgggcgcg
     2101 tccggctgct cgaacccgaa ggcgtctcgg tcgtctccga catcgacgac accatcaagg
     2161 tcacgaacgt tcccgccggc acggagaccg tcctgcgccg ctcgttcctg atggacttcg
     2221 aggcggcgag ctacacgttc aaggccgagg gcaaggaggc cgccaagcgg cagatgcgcg
     2281 aactctactg ggagctgcac gccgagcacg aggccgcgga cgtggccttc cactacgtct
     2341 cgggcagccc gtggcagttg ttcaggctgc tggacgactt cctcgtccgc cgccgcgaag
     2401 gcttccccga aggcaccttc cacatgaaga gcctgcgcaa gaacctgacc gaccccgaca
     2461 cgtgggccga tctgttcggc ctcgcgcagg gcaagaaggc cacgctcgaa cagaagatca
     2521 agcagatcac cgagctgatg atcaacctgc cccgccgccg cttcatcctc atcggcgact
     2581 cgggcgagat ggacccggag gtcttcctcg ccatgaacga gatgttcccc aaccatgtcg
     2641 cgcgcatcat catccgcgac gtggtcggcg agcgcctcaa gcacccgaag atcgaagtgc
     2701 tgcccggcga caccgtccgc tacgacaccc ggaaggtgct caaaaaaatc atccgggaga
     2761 tggcgctcga catcaagatc gaacaggccg ccgagccgcc gcagaccgag ggctgaacaa
     2821 ccagagcggg ttcaaagtag tgtgagacgc gcgtgatgac tgtaggggca gggacaagcc
     2881 ctgcccctac agtcatca
//
LOCUS       DBNGOH010000153         2022 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1672171, whole genome shotgun sequence.
ACCESSION   DBNGOH010000153 DBNGOH010000000
VERSION     DBNGOH010000153.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2022)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2022)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2022
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1672171"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..653)
                     /locus_tag="AC159P_02765"
     CDS             complement(<1..653)
                     /locus_tag="AC159P_02765"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HIR3694405.1"
                     /translation="MKILDRMVMEQYDSFKVIIFFVALTVLVTGLVVVVWEKVFMLPF
                     YAWIAPYSGGEGSAAYYFQQRIEHFLISTTVDVLIVTLLLRIMSRQQSKLVASEKRYR
                     ALFEHASDGIGVITADDHRLVEVNNKFCDILGCEPKLLIGRDIRTLLRVDGDERGRDR
                     LAELLSGATTGDEVVVTIRTENGGSIATAVSSSELLTDGEKLVILIVHDLSERRRLEA
                     "
BASE COUNT          355 a          637 c          645 g          385 t
ORIGIN      
        1 gcttcgagcc gcctgcgctc cgagaggtcg tggacgatga ggatgaccag cttctcgccg
       61 tcggtcagga gttcgctcga agagaccgcc gtcgcgatgg agccgccgtt ctccgtcctg
      121 atcgtcacca cgacttcgtc gccggtggtg gcgccgctca ataactccgc gagccggtcg
      181 cgtccccgtt cgtcgccgtc cacgcgcagg agggtgcgga tgtctctgcc gatgagcagc
      241 ttcggctcgc agccgaggat gtcgcagaat ttgttgttga cctcgacgag gcggtggtcg
      301 tccgccgtga tgaccccgat gccgtccgag gcgtgctcga acagcgcgcg gtagcgcttc
      361 tcgctggcga cgagcttgct ttgctgccgg ctcatgatgc ggagcagcaa cgtgacgatg
      421 agcacgtcca ccgttgtcga gatgaggaag tgctcgatcc gctgctggaa gtagtaggcg
      481 gcgctcccct cgcccccgct gtacggcgcg atccacgcgt agaacgggag catgaagacc
      541 ttctcccaga cgactacgac gaggccggtg acgaggacgg tgagggcgac gaagaagatg
      601 atgaccttga agctgtcgta ctgttccatc accatccgat caagaatctt catgggcgtc
      661 accctcccgc tctttcccgc accgcgtgcg tacgccgcgc ccgcgccgcg cggtttggcc
      721 ggcgcgacgg cggggcggcg gcagtccttc gctcaggtcg gtagacttgg ggctagccct
      781 tcagaaaggc gtgagatgaa gcctgaacgt cggggaatca gaagcctttg ggcgcgggcc
      841 gcgcgcgagg gtcagcagtg acgtgcgccc gcgttaagac gaaaatcgga tgaaactttc
      901 ggcgaccgcc tccgggccgg atgccggtcg caggtttcgt gagccggcga agtatgacag
      961 gaagcaaagt attgatgctt cgcgggatgc taaatttttt gcggaaggcg tgtcaagcct
     1021 cgggcgcgcg gcggcgcggg cggtcctgcg agttgtcgga ctccgccggc ggtctgttgc
     1081 ggcgactggc gcgggcgatt gaggcaatta tttcatcatt ataatttcat taccggcgac
     1141 gcagcacaca ggcgcaccgc tccgtacgtg agtagacaga ccgcgacctg gcttggcaag
     1201 ccgggtgcca ttcgtgcccg ttcgcaacgc ctgtaatttc gcagaagaga ggcggcggcg
     1261 aggcttcctg tgcgcgggtc gttgatgcgt gctgaaacag ggggcactcg aagtgtttca
     1321 cggtggaaca cgcccggcgg cgatgcccac gcgcgctctt cgttgcaagt tcaataagaa
     1381 tccatgacac ttctttacta ttccccccgc gagactctgc ggcacagccc ttgcccctgc
     1441 ccatatctcc tgctgacctt actcacgaag taagcaacgc gccgcgcaag cagttaccac
     1501 gcgcggtctc ggagccttag ccaaaccaat ccacccgatg cctcgcgcgg cggatgacct
     1561 tctgttgacg gtgacgccga cgtgtgggcg cgaacaggac gtacttctta tcttggagtt
     1621 cagtttagag atgacaaaac cacgtcgctt gagacacgca tcttacgtcc gcctggtttg
     1681 tctccttctc tgcttatcac ttgcggcgcc ctccgccgcc gtctccgccg cgttgagcac
     1741 tccccggaag gccgtcgagt cccacgccgg aagcggctcg gagcgtgact tctccgccac
     1801 cgcaagtggc aacgaggccg cttccttcgc cgccgcgcgc agtgaagtag cctcgtcgaa
     1861 ccgagccatg gagggcgcgc gagccggagc aaagctcgcc gccaccttca cggaggttca
     1921 gctcggaagg actgttgaca aacgtggcgt tgacgcaacc cactctcttt ccaccgcccg
     1981 gaggaaggcc gcgaacgcgc ccgttcggtc gtctctggca gg
//
LOCUS       DBNGOH010000154         4262 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2405585, whole genome shotgun sequence.
ACCESSION   DBNGOH010000154 DBNGOH010000000
VERSION     DBNGOH010000154.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4262)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4262)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4262
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2405585"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(42..1028)
                     /locus_tag="AC159P_02770"
     CDS             complement(42..1028)
                     /locus_tag="AC159P_02770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694406.1"
                     /translation="MCRRLFSAAGCLALVLASAALTFAQDTPTQPQPTQQPTQTTTVT
                     QTTQAVQNPDGTWTVVQYPANKEVVVDFTPDTTLATAKGRAKVMRMGDHTMINVDLSG
                     VPAGVTSYNVYAVDPFGKATLLGPVQIANGAATHEFKTGLDRFMLVLSPDAGLTTYTP
                     TSTTAVAFRSAVPQGMAVVPISHSDISPGAATGEKVSATTTATTGYNVPMLNVAGMKR
                     GENTEVKVNFTGAMTGARANFNIEPRKDGPTMVKARFHELKEAPAGQIYTLWAVSPDN
                     KFVKLGQIVNTGTRNEAEIKSETTLPEFGLLVTLEEGEQASPHGVVVGTSVP"
     gene            complement(1306..3426)
                     /locus_tag="AC159P_02775"
     CDS             complement(1306..3426)
                     /locus_tag="AC159P_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013568547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045240 - alpha-ketoacid dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit alpha/beta"
                     /protein_id="HIR3694407.1"
                     /translation="MATEIKAREYRGLGREEMVGIYRTIYLSRRLDDKEKDLKGQNRI
                     FFQISGAGHEAVLTAAGLAMKSGYDWFYPYYRDRALCLQLGVTPLEMLLAAVGAEADP
                     SSHGRQMPSHWGHRKFNIVTQSSPTGTQFLQAVGAAEASYRASLVEEIKERVAGFKGD
                     EVVYCSAGDGTTSEGEFWEALNTASNLKLPVLFLIEDNGYAISVPVEVQTAGGDISKL
                     VAGFPDLFIQKCDGTDPVESVETLKRAVAYCRERKGPALVHASVIRPYSHSLSDDEKL
                     YRSDEERTSDAERDPVKKFGALLIDEGFVTQEELQKIKDDIDREVSEATDAALASAQP
                     DPSTATDFVYSPDVDPTAPEFDTEEGAELSGNAGTMVDLINRCLHEEMARDPGLVVFG
                     EDVADVSREQHLDKVKGKGGVFKVTANLQRKFGSARVFNSPLAEANIVGRAIGMATRG
                     LKPVVEIQFFDYIWPAYHQIRNELALIRWRSGNEWKCPVVIRVPVGGYLKGGAVYHSQ
                     SGDTIFTHVPGLRVVYPSNALDANGLLRTAIRCDDPVIFFEHKHLYRQAYNKSQYPPD
                     GFMIPFGKARTVREGADLSIITYGALVQRSLVAAKQAEQQQGISVEVIDLRTLAPYDW
                     ESVARAVKKTGKLIVAHEDTRTHGFGAEIAARVADELFEYLDAPVRRVAALDTFVAYA
                     PQVEDAILPQVEDVARAITELYSY"
     gene            complement(3454..3696)
                     /locus_tag="AC159P_02780"
     CDS             complement(3454..3696)
                     /locus_tag="AC159P_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HIR3694408.1"
                     /translation="MKLPRDVSGDDLAKALKALGYHVTRQSGSHLRLTTTEGGDHHIT
                     IPRHDPVRIGTLAAILDDVAEHFEISRDELLTRIKR"
     gene            complement(3693..3899)
                     /locus_tag="AC159P_02785"
     CDS             complement(3693..3899)
                     /locus_tag="AC159P_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017286718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoisovalerate dehydrogenase"
                     /protein_id="HIR3694409.1"
                     /translation="MEELIFLVEEAPEGGYTARALGASIFTEADDLDGLREMVRDAVR
                     CHFDEGQGPRVIRLHFVREEVFAA"
     gene            complement(3905..4225)
                     /locus_tag="AC159P_02790"
     CDS             complement(3905..4225)
                     /locus_tag="AC159P_02790"
                     /inference="COORDINATES: protein motif:HMM:NF024579.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3694410.1"
                     /translation="MAASRIDIFRMMLEGDPDNTSVMFGLAKEYEKAGRFDEMIEALS
                     DYLQRADDEGNAYGMLAAAYLKIGRRDAARKAYERGAEVALAHGHPSMAADYRMMIET
                     EFED"
BASE COUNT          691 a         1370 c         1394 g          807 t
ORIGIN      
        1 cggcgttgaa gggagcgcgt gttgatgtgg gccgctttcg gttacggtac gctggtgccg
       61 acgacgacgc cgtgcggcga agcctgttca ccctcttcga gcgtcacgag cagcccgaac
      121 tcgggcagcg tcgtctcgga cttgatctcc gcctcgttgc gggtgccggt gttgacgatc
      181 tggccgagct tgacgaactt gttatcgggc gagacagccc agagcgtgta gatttgaccg
      241 gcgggcgcct ccttcagctc gtggaagcgc gccttgacca tcgtcgggcc gtccttgcgc
      301 ggctcgatgt tgaagttggc gcgcgcgccc gtcatcgcgc cggtgaagtt gaccttgacc
      361 tctgtgttct cgccgcgctt catccccgcg acgttgagca tcggcacgtt gtagccggtg
      421 gtcgcggtcg tcgtggccga gaccttctcg ccggtggcgg cgccgggcga aatgtcgctg
      481 tgcgagatcg gcaccacagc catgccctgc ggcacggcgc tgcggaaggc gaccgcggtc
      541 gtgctcgtcg gcgtgtaggt cgtcaggccg gcgtcgggcg agagcacgag catgaagcgg
      601 tcgaggccgg tcttgaactc gtgtgtcgcc gccccgttcg cgatctggac ggggccgagc
      661 agcgtggcct taccgaacgg atcgaccgcg tacacgttgt aactcgtgac gcccgcgggc
      721 acgcccgaga ggtcaacgtt aatcatcgtg tggtcgccca tccgcatcac cttcgcgcgg
      781 cccttcgcgg tcgcgagcgt cgtgtcgggc gtgaaatcga cgacgacttc cttgttggcc
      841 gggtactgga cgaccgtcca agttccgtcg gggttctgga cggcctgcgt ggtctgcgtg
      901 acggtggtgg tctgcgtggg ttgctgagtc ggttgcggct gggttggcgt gtcctgcgcg
      961 aaggtcaacg ccgccgaggc gagcaccaac gcgaggcagc ccgccgcgct aaacaaacgt
     1021 ctgcacatta ctatctccct cctacaatct taacgatttc cccttgcgcg tatcgtccgc
     1081 cctgaaaaat gacggaacaa gtaacaaacg gcaacgacca tgccgctcgg cgcaggtgac
     1141 gacgggacaa caacttaggg gaaacgcggg cggcgggcgc aggagcgggc gaagcgatac
     1201 gatacaaacg tgtacgacga tgcggcgacg ggagggaaga aggggaaaag gagaagaagc
     1261 aacttgcttt gctcctttcc cccttttcgc cttttccccc tgtctttagt agctgtacag
     1321 ctccgtgatg gcgcgggcca cgtcttcgac ctgcgggagg atggcgtctt cgacctgcgg
     1381 cgcgtaggcg acgaaggtgt cgagcgcggc gacgcggcgg acgggcgcgt cgaggtattc
     1441 gaacagctcg tcggcgacgc gcgcggcgat ctcggcgccg aagccgtgcg tgcgcgtgtc
     1501 ctcgtgcgcg acgatgagtt tgccggtctt cttcaccgcg cgcgcgaccg attcccagtc
     1561 gtagggcgcg agtgtgcgca ggtcgatgac ctcgacgctg atgccctgct gctgctcggc
     1621 ctgcttggcg gcgacgagcg agcgctggac gagcgcgccg taggtgatga tcgagaggtc
     1681 agcgccctcg cgcactgttc gagccttgcc gaacgggatc atgaagccgt cgggcgggta
     1741 ctgcgacttg ttgtacgcct gccggtagag gtgtttgtgt tcgaagaaga tcacggggtc
     1801 gtcgcacctg atcgcggtgc gcagcaggcc gttcgcgtcg agcgcgttcg aggggtagac
     1861 gacgcgcagg cccggcacgt gcgtgaagat ggtgtcgccc gactgcgagt ggtagacggc
     1921 gccgcccttc aggtagccgc cgacggggac gcggatgacg accgggcact tccactcgtt
     1981 cccggagcgc cagcggatga gcgccagctc gttcctgatc tgatggtagg cgggccagat
     2041 gtagtcgaag aactgtatct cgaccaccgg cttcagcccc ctggtcgcca tgccgatggc
     2101 gcgcccgacg atgttcgcct cggcgagcgg cgagttgaag acgcgggccg agccgaactt
     2161 gcgttgcagg ttcgccgtga ccttgaagac gccgcccttg cccttcacct tgtcgaggtg
     2221 ctgctcgcgc gaaacgtcgg ccacgtcctc gccgaagacg actagccccg ggtcgcgcgc
     2281 catctcttcg tgcaggcaac ggttgatgag atcgaccatc gtgcccgcgt tgccggaaag
     2341 ctcggcgcct tcttcggtgt cgaactccgg ggcggtcggg tcaacgtccg gcgagtagac
     2401 gaagtcggtc gcggtcgagg ggtcgggctg cgcgctcgcg agcgccgcgt cggtcgcctc
     2461 gctcacctcg cggtcgatgt cgtccttgat cttctgcaac tcctcctgcg tgacgaagcc
     2521 ctcgtcgatg agcagcgcgc cgaacttctt gaccgggtcg cgctccgcat ccgacgtgcg
     2581 ctcctcgtcc gagcggtaga gcttctcgtc gtccgagagc gagtgcgagt aggggcggat
     2641 gaccgacgcg tggacgagcg ccgggccttt gcgctcgcgg cagtaggcga ccgcgcgctt
     2701 cagcgtctcg acggactcca cggggtccgt gccgtcgcac ttctggatga acagatcggg
     2761 gaagcccgcg accagcttcg agatgtcgcc gcccgccgtc tgcacctcga cggggacgct
     2821 gatggcgtag ccgttgtcct cgatgaggaa gagcaccggc agtttcaagt tgctcgccgt
     2881 gttcagcgcc tcccagaact cgccctcgct cgtcgtcccg tcgcccgcag agcagtagac
     2941 cacctcgtca cccttgaagc ccgcgacccg ctctttgatc tcttcgacga gcgacgcgcg
     3001 gtaactcgcc tccgccgcgc ccacggcctg caaaaactgc gtgcccgtcg gcgacgactg
     3061 cgtcacgatg ttgaactttc ggtgccccca gtgcgaaggc atctgccgac cgtgcgagct
     3121 ggggtcggcc tcggccccga ccgccgccag cagcatctcc aaaggcgtca cgcccaactg
     3181 caagcacagc gcgcggtcgc ggtaataagg gtagaaccag tcgtagcccg acttcatcgc
     3241 cagccccgcc gccgtcagca ccgcctcgtg acccgcgccc gaaatctgga agaaaatcct
     3301 gttctgcccc ttcaaatcct tctctttatc gtccagccgc cgcgacaggt agatcgtccg
     3361 gtaaatcccg accatctcct cgcggcccaa gccacgatac tcgcgcgctt tgatctcagt
     3421 cgccatgatt actaattctc ctacttcgaa taatcagcgc ttgattcttg tcaataactc
     3481 atcgcggctg atttcgaagt gctccgccac gtcgtccagg atcgcagcaa gcgtgccgat
     3541 gcggacgggg tcatgacgtg gaatcgtaat gtggtggtca ccgccctccg tcgtggtcag
     3601 gcgcaaatga ctgcccgact gccgcgtgac atgatagccg agcgccttga gagctttggc
     3661 gaggtcgtcg cccgaaacgt cgcgcggcaa tttcatgcgg cgaacacttc ttcacgcacg
     3721 aaatgaagac gaatcacccg cggcccctgt ccttcgtcaa agtggcaacg caccgcgtca
     3781 cgaaccatct cccgcaggcc gtccaggtcg tcggcctcgg tgaaaatcga agcccccaag
     3841 gcgcgcgccg tgtagccgcc ttcgggtgct tcctcgacga gaaagatcag ctcttccatg
     3901 tcgctcagtc ttcgaactcg gtctcgatca tcatccggta gtcggcggcc atcgaggggt
     3961 ggccgtgggc gagggcgact tcggcgccgc gctcgtaggc tttgcgggcg gcgtcgcgcc
     4021 tgccgatctt gaggtaggcc gcggcgagca tcccgtaggc gttgccctcg tcgtcggcgc
     4081 gctggaggta gtcgctgagg gcctcgatca tctcgtcgaa gcgcccggcc ttctcgtact
     4141 ccttcgccag cccgaacatc accgaagtgt tgtccgggtc gccttcgagc atcatgcgga
     4201 agatgtctat gcgtgaagcg gccataatgt gaaagccgtg aaccgtcaac cgtcaaccgt
     4261 ga
//
LOCUS       DBNGOH010000155         7324 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3322050, whole genome shotgun sequence.
ACCESSION   DBNGOH010000155 DBNGOH010000000
VERSION     DBNGOH010000155.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7324)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7324)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7324
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3322050"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..652
                     /locus_tag="AC159P_02795"
     CDS             <1..652
                     /locus_tag="AC159P_02795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694411.1"
                     /translation="SELFASFGARRRAAVTRYHIALTVATALAAFIATLYAFAWQPRP
                     TAGGQPPPAPAGAAPNVTSQPAAPAQTAAPNPQPSPFAPAQGAQGGPVIILDPPADVP
                     PVGTNAAAGQSAAPANSPPAVVSEGGAQTPEGEAPRTEATNPADSLRAGEAGKKDKPK
                     GDDARAGEEEPRRGSQEGQGAEIRTAAPAARPSAQPSSPARAQPSPTPKKKVIQWP"
     gene            754..1392
                     /locus_tag="AC159P_02800"
     CDS             754..1392
                     /locus_tag="AC159P_02800"
                     /inference="COORDINATES: protein motif:HMM:NF041621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MXAN_5187 C-terminal domain-containing protein"
                     /protein_id="HIR3694412.1"
                     /translation="MPIRDNKFARRLKEREKLKPKTAGRDEQPSVDEQLARLEEDVRR
                     LKVEFDIYFNGAAKRPPYDTKSRVDTLIKRLADDRTLSYAQRYRYNSVVARYNSFLQL
                     WRRTLQDREEGRGAAGARRTAKVEEEAAPPQTFSCADPHKDVTTVKRLFESLVEAKKS
                     CGEPTEELSFPRFHRMIASQADALKDKAGCGSVQFSVGVKDGRVSFKARPEK"
     gene            complement(1620..1826)
                     /locus_tag="AC159P_02805"
     CDS             complement(1620..1826)
                     /locus_tag="AC159P_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011233297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HIR3694413.1"
                     /translation="MSRITGKVKWFNNAKGYGFIEQPGSSDIFVHYSAIQGDGFKTLE
                     EGQTVEFEVTQGPKGPQAEKVIKM"
     gene            complement(2202..3056)
                     /locus_tag="AC159P_02810"
     CDS             complement(2202..3056)
                     /locus_tag="AC159P_02810"
                     /inference="COORDINATES: protein motif:HMM:NF013700.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HIR3694414.1"
                     /translation="MNRDERFYAFIVATTSRSRSQIRRISVHKRWLKVSAVAAFVVLC
                     ATAYGFYGLAQQASHIRIEQENDRLRRENNRQREKLRTLENRVDAIEDEARRLSEISG
                     VTHEEDGAATPGTGGPSMLLDEAGIAAIELRAAQLEADLKAHEAALTERSRVPSIWPV
                     EGGEVTDTFGGRRDPFGGGSEFHSGQDIRAPRGTPVHASANGVVTLAGWQNGYGNTVV
                     IDHGNGLTTRYGHLYKIDAVLGQEIKRADSIGQVGSTGRSTGPHLHYEVRINDVAVNP
                     GHYLPARW"
     gene            complement(3480..3698)
                     /locus_tag="AC159P_02815"
     CDS             complement(3480..3698)
                     /locus_tag="AC159P_02815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694415.1"
                     /translation="MSSLLVTAGKWGGILTLIALLIALVKQLITLVGFIMFFLKAALV
                     IAFVGLMLLIVVTMMRTRSRRRREAEEL"
     gene            complement(3852..4646)
                     /locus_tag="AC159P_02820"
     CDS             complement(3852..4646)
                     /locus_tag="AC159P_02820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694416.1"
                     /translation="MNCAYHLTNRAVVQCSRCTRPLCPACDHRIRGFPYCQDCIVAGV
                     ESLQHQQARVAAKSVVRRKSSPFVALLLSLFCPGLGAAYNGQTAKALVHFTIFASFFQ
                     MATVTDGTAFFVLGVVGTWLFAAVDAARTAQLIRAGLAPDAEQDAIARRLYGNPLAWA
                     VTMMSLGIVFLLHTIFGVQLPVREFLPLVLVLLGAYMLFDYFRARRRRDPLQFEGVSR
                     PPSLFGTTAPAAGALAAGEMTRFGTGELVTQVSARESSLWPNEPRA"
     gene            complement(4777..5586)
                     /locus_tag="AC159P_02825"
     CDS             complement(4777..5586)
                     /locus_tag="AC159P_02825"
                     /inference="COORDINATES: protein motif:HMM:NF024881.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor family protein"
                     /protein_id="HIR3694417.1"
                     /translation="MLCVEFEDRLTDYLDGALEVQTQRVFAEHAMRCPVCHELLNEVK
                     NALTECRASAAPPPSPGLEARIIMKTAPETSMSCTEFEEHLTDYLDGFLPAALYHRWE
                     RHAALCESCTELPGQVVRAIGACYSYISEEKPLPVGLHERILQATLGTTEAERVRAPF
                     PSRVAAMLRDWLDAIVQPQLATVATMILVAVLVGTTTISDDGSIGGMYRASLRLAAQT
                     ANQTDGLKRVTEGLGTLVGSSADKGAAEMKPAPAENDGAKSDEQQTEEKDR"
     gene            complement(5722..6306)
                     /locus_tag="AC159P_02830"
     CDS             complement(5722..6306)
                     /locus_tag="AC159P_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR3694418.1"
                     /translation="MTVGEGAELVRRCRAGDGAAWEEIVSTYSRRIYNLAYRFTSRAD
                     AAEDLTQEVFVRVYRSLEQYDAKQGDLQKWLMRLARNLIIDDYRRRQRAPQDTQAEDI
                     EGHTYHLRAAGNTPQREMERRELGEQVQAAIDKLPPDLRTCVILRDIEELSYQEIVDH
                     LKIPEGTVKSRINRGRIELAKILRRMRVVAMSEV"
     gene            6779..>7324
                     /locus_tag="AC159P_02835"
     CDS             6779..>7324
                     /locus_tag="AC159P_02835"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015651.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HIR3694419.1"
                     /translation="MGKYLKFIVLCVLAALVLVWFGRGLDWAEVSGAIGRADWRMVAA
                     GVVIVWATYLIRAFRWRTLLKPLAPRASLRETFGATTVGFAAIFLVGRAVGEVLRPAF
                     LAVRDPEVKPGPAFVTIAVERIYDSVAMVVLFAANLLVFRPATADAAGLQRLRLIGLV
                     LLAGAVVGVAALALFRRHAGKV"
BASE COUNT         1236 a         2328 c         2513 g         1247 t
ORIGIN      
        1 gagtgaattg ttcgcgagtt tcggtgcgcg tcgtcgcgcc gcggtgaccc gctatcacat
       61 cgcgttgacg gtcgcgacgg cgctggccgc cttcattgcc acgctgtacg ccttcgcgtg
      121 gcagccaagg ccgacggcgg gtgggcagcc cccgcccgcc cccgcgggcg ccgcgccgaa
      181 cgtcacgtcg cagcccgcgg cgccggctca gacggcagcg ccgaacccgc agccgtcgcc
      241 gtttgcgccg gcgcagggcg cgcagggcgg gccggttatc atcctcgacc ccccggcgga
      301 cgttccgccc gtcgggacga acgccgccgc gggccagtcg gcggcgcccg ccaactcgcc
      361 gcccgccgtc gtgtcagagg gcggcgcgca gacgccggag ggcgaggcgc cgcgcaccga
      421 agcgacgaac cccgcggaca gcctgcgcgc gggcgaagcg ggtaagaagg acaagccgaa
      481 aggcgacgac gcgcgcgcgg gcgaggaaga gcctcggcgc ggctcgcagg aggggcaggg
      541 cgcggagatt cggacggcgg cgcccgccgc gagaccttcc gcgcagcctt cgtcgcccgc
      601 gcgggcgcaa ccttcgccca cgccgaagaa gaaggtcatc cagtggcctt gacgggcgcc
      661 ggagtttatt gacaccgcga gaggcgaagc tatagattcg gagaggcgcg tccgacgaag
      721 ctcgcgcgcc tcgaacgcga cacggagacg aaggtgccca tccgagataa caagttcgcg
      781 cggcggctca aagagcgcga gaagctgaag ccgaagacgg cgggccgcga cgagcagccg
      841 agcgtggacg aacaactcgc ccggctcgaa gaggacgtgc gccgcctgaa ggtcgagttc
      901 gacatctact tcaacggcgc cgccaagcgc ccgccctacg acacgaagtc gcgcgtggac
      961 accctcatca aacgcctcgc cgacgaccgc acgctctcct acgcgcagcg ctaccgctac
     1021 aactcggtcg tcgcgcgcta caactcgttc ctccaactct ggcggcgcac gctccaggat
     1081 cgcgaagagg ggcgcggcgc cgccggcgcg cggcgcacgg cgaaggtcga ggaagaggcc
     1141 gcgccgccgc agaccttctc ctgcgccgac ccgcacaagg acgtgacgac cgtgaagcgc
     1201 ctgttcgagt cgctcgtcga ggcgaagaag agctgcggcg agccgaccga ggaactctcg
     1261 ttcccgcgct tccaccgcat gatcgcctcg caggccgacg cgctcaaaga caaggcgggc
     1321 tgcggcagcg tccagttctc cgtcggcgtc aaagacggtc gcgtgagttt caaggcgcga
     1381 ccggagaagt aaagcagtag gcagtaagca gtaggcggta ggcagtcggg aacaagcagc
     1441 gaacagttaa aaacagcgag cggtgagtag tagatgactt ctactgctca ccgctcgctg
     1501 cttattgcct acttggcttt cttgagtgcc ttgcttggta gtgcgcttgc cggctcttcg
     1561 cggagagtga ccggaagcgg tcagtggcct tcaacgaaag ctgaagttca atggggagat
     1621 tacatcttga tcactttttc cgcctgcggc cctttcggcc cctgcgtcac ttcgaattcg
     1681 acggtctggc cctcttcgag cgtcttgaag ccgtcgccct ggatggcgct gtagtgaacg
     1741 aagatgtcgg aagagccggg ctgctcgatg aagccgtagc ccttcgcgtt gttgaaccac
     1801 ttgaccttac cggtaattct ggacatagct aacgaatcct cctaacgatt cggtgaaagc
     1861 gagacgggca atcgcttggc cgcacccgtc cgcgaagttg actactcacg agccggttcg
     1921 cgcgcccctt ggacgaaggc acgcgccgaa aactccaacc aaacaaaaag cccgcggttg
     1981 acggccactc gcgcactcgc gcgccacgga ctcgtcatac gcggactgga gacggggtac
     2041 tgccgaaaac ttcatcgccg tattgcttgc aaacctaaca ggggggcggg tttaaggcgc
     2101 tccccgtcct gaaacgacgg cgcatactaa gcgaaaccgt gcgaggctgt caagcataaa
     2161 atacgcgccg ttcaaggctt ttaccgcgtc cggcgccgaa atcaccaacg ggccggcagg
     2221 taatgaccgg ggttgacggc cacgtcgttg atgcgcactt cgtagtgcag gtgcggcccc
     2281 gtggagcggc ccgtcgagcc gacctgcccg atcgaatcgg cgcgtttgat ctcctgcccg
     2341 agcacggcgt cgatcttgta gaggtggccg tagcgtgtcg tcagcccgtt gccgtggtcg
     2401 atgacgacgg tgttgccgta gccgttctgc cagcccgcga gcgtgacgac gccgttggcc
     2461 gaggcgtgga cgggcgtgcc gcgcggcgcg cggatgtcct gtcccgagtg gaactccgag
     2521 ccgccgccga acgggtcgcg gcgaccgccg aaggtgtccg tcacctcgcc gccctcgacc
     2581 ggccagatcg aaggcacgcg cgagcgctcc gtgagcgcgg cctcgtgcgc cttcaggtcg
     2641 gcttcgagct gcgcggcgcg cagttcgatg gcggcgatgc cggcctcgtc gagcagcatc
     2701 gaggggccgc ccgtgccggg agtcgcggcg ccgtcctcct cgtgcgtgac gcccgagatt
     2761 tcggagagcc tgcgcgcctc gtcctcgatg gcatcgacgc ggttctccaa cgtccgcagc
     2821 ttctcgcgct ggcggttgtt ctcgcgccgc aggcggtcgt tctcctgctc gatgcggatg
     2881 tgcgaagcct gctgcgcgag gccgtagaag ccgtaggccg tggcgcagag gacgacgaag
     2941 gccgcgaccg cggagacctt gagccagcgt ttgtggacgg agatgcggcg tatctgggaa
     3001 cgtgagcgcg aagtggtggc gacgatgaaa gcgtaaaaac gctcgtctct gttcatcgga
     3061 gtgtgttcct tgggcgccct gcccacacgg gtagcctggg gggcgaaccg cgcgcatgag
     3121 cgcgaaaggc actgtctgcc gaaagaaggc gacgagtgcg gaaacctgat aattttgggt
     3181 tcttacttag tgcgagaccc ttcctgaatg tgccagtcgc gcgggccaat ctcgtgtgat
     3241 atagcagata cgagcgaccg atcagaactg cacaaggggc tgcaccctat cacacggggc
     3301 gggtgattgc aaccaacgag gcaaattgca atgtggtcgc aacgaagagg aaacaatgtg
     3361 cgggggaaga ggggaaaagg gaaaaaggtg aagaggcgaa gagggaagac gaggagcagc
     3421 actcctctca tcctccctct tcgcctctta acctttttcc cttttcgccc ttccgggtct
     3481 taaagctctt cggcctcgcg gcgacggcgg ctgcgcgtcc gcatcatcgt gacgacgatg
     3541 agcagcatca ggccgacgaa ggcgatgacg agcgcggcct tgaggaagaa catgatgaag
     3601 ccgacgagcg tgatgagttg tttgacgagc gcgatcagga gcgcgatgag cgtgaggatg
     3661 ccgccccact tgcccgcggt cacgagtaat gaagacattt cggagaactc cttccttgat
     3721 tgaaaaccga actcgaaagc tttcgcgctt tcccaacctt cgtgcgtcgc gccttcggcg
     3781 cggggcgacc gtcgccatca taacaaagaa cgccgacgcg ccgcgaggtt tgcgtcctcg
     3841 ccgcgcgccg ctcacgcgcg cggctcgttc ggccagaggc tgctctctct ggccgagacc
     3901 tgcgtgacga gttcgcccgt gccgaagcgc gtcatctcgc ccgccgcgag ggcgcctgcc
     3961 gcgggggcgg tcgtgccgaa gagagagggc gggcggctca cgccctcgaa ctggagcggg
     4021 tcgcggcggc ggcgcgcgcg gaaatagtcg aagagcatgt aggcgccgag caggacgagc
     4081 acgagcggga ggaattcgcg cacgggaagc tggacgccga agatcgtgtg gagcaggaag
     4141 acgatgccga gcgacatcat cgtcacggcc cacgcgagcg ggttgccgta gagtcggcgc
     4201 gcgatggcgt cctgttcggc gtcgggcgcg aggccggcgc ggatgagctg cgccgtgcgc
     4261 gccgcgtcca ccgccgcgaa gagccacgtg ccgacgacgc cgaggacgaa gaaggccgtg
     4321 ccgtcggtga cggtcgccat ctggaagaag ctggcgaaga tcgtgaagtg gacgagcgcc
     4381 ttcgcggtct ggccgttgta ggccgcgccg aggccggggc agaagagcga gagcaggagc
     4441 gcgacgaaag gcgaactctt tctgcgcacg accgacttgg cggcgacgcg cgcctgctgg
     4501 tgttgcagcg actcgacgcc cgcgacgatg cagtcctggc agtacgggaa gccgcggatg
     4561 cggtggtcgc acgcggggca cagcgggcgc gtgcagcggc tgcactgcac gaccgcgcgg
     4621 ttcgtcaaat ggtaagcgca gttcatcaag tccgttacga aatctctgcg ttcttcaagc
     4681 tcgaccgcgc ccgccggctc gtcgcgagcc aggcccgagc gcgtcgggca gcacgattgt
     4741 caaacgccgc cgttcggcct cgccgaaggg cggcgctcag cggtctttct cctcggtctg
     4801 ctgttcgtcc gacttcgcgc cgtcgttctc ggcgggcgcg ggcttcatct cggcggcgcc
     4861 tttgtcggcg gacgagccga cgagcgtccc gagtccttcg gtgacacgct tgaggccgtc
     4921 cgtctggttc gccgtctgcg cggcgaggcg gaggctggcg cggtacatgc cgccgatgga
     4981 gccgtcgtcc gagatcgtgg tggtgccgac gagcacggcg acgaggatca tcgtcgccac
     5041 ggtggcgagc tgcggctgga cgatggcgtc gagccagtcg cgcaacatcg cggcgacgcg
     5101 cgaggggaac ggcgcgcgca cgcgctcggc ctcggtcgtg ccgagcgtgg cctgcaagat
     5161 tctctcgtgg aggccgacgg gcaggggctt ctcttcgctg atgtaggagt agcaggcgcc
     5221 gatggcgcgc acgacctgtc cgggcagctc cgtgcagctt tcgcagagcg cggcgtggcg
     5281 ctcccagcgg tggtagagcg cggcgggcag aaagccgtcg aggtagtccg tgaggtgctc
     5341 ctcgaactcg gtgcacgaca tcgaagtctc gggcgcggtc ttcatgatga tgcgcgcctc
     5401 aagccccggc gaaggcggcg gcgccgccga cgcgcggcac tccgtgagcg cgttcttgac
     5461 ctcgttcaga agctcgtgac agaccgggca gcgcatcgcg tgctcggcga aaacgcgctg
     5521 cgtctggact tcgagcgcgc cgtccaggta gtcagtcagc cgatcttcaa attctacgca
     5581 cagcatcttt gaattctctc caccctctcg actcctaatg ctgaacgcag aatgcagaat
     5641 gatgaatgag aagcaacgcg cctctcgtca cgcagtttgt cttcccattc atcattcatc
     5701 attccgcacc cgtcatttcc ttcacacctc gctcatggcg acgaccctca tgcgccgcaa
     5761 aatcttcgcc agctcgatgc gcccgcggtt gatccgcgac ttcacggtgc cctcgggtat
     5821 cttcaggtga tcgacgatct cctgatagct cagctcctcg atgtcgcgca ggatgacgca
     5881 cgtccgcaag tccggcggca gcttgtcaat cgcggcctgc acctgctcgc ccagctcgcg
     5941 ccgctccatc tcgcgctgcg gcgtgttgcc ggcggcgcgc aggtggtagg tgtggccctc
     6001 gatgtcctcg gcctgcgtgt cctgcggggc gcgctgtctc cgccggtagt cgtcaatgat
     6061 caagttacgc gcgaggcgca tcaaccattt ttgcaggtcg ccctgcttgg cgtcgtactg
     6121 ttcgagcgag cggtagacgc ggacgaaaac ctcctgcgtc aaatcctcgg cggcgtcggc
     6181 gcgcgaggtg aagcggtacg cgaggttgta aatccggcgc gagtaggtgg agacgatctc
     6241 ctcccacgcg gcgccgtcgc ccgcgcgaca cctcctgacc agttcagccc cttcgccgac
     6301 agtcacggcc ctagtaactc ccacctcgcg ccccttccgt ctccctccgc cgtctcatcc
     6361 cttgcgagaa cacgcgtggg tggtcgaaac cccttgccac acgggcatta cgaccgccgg
     6421 acgggtaaag ttcccgtgcc ataagtaggc ggtaggcggt agggagtagg cggttaaagc
     6481 cgctccgccc gtctcgtggt tcgcctactt gggcagtctt gggaggcggc taacagccgt
     6541 accgggagcc gggttcaagt ctcgttagct ctcgacggac tgcttactgc ctaccgccta
     6601 ctgcctactg ttttacgcct tgcgccgccg cgtttaagta ttatagccta gctgcgttac
     6661 actatatctt gtggcggcga attggcgaag tcaaatttag gggcggagcg cggcgaggtt
     6721 tgtcgggaga gcggcttgcg ggccgccgtc gccggtctcg gcagtagaag tttatcgctt
     6781 ggggaaatat ttaaaattca tcgttctgtg cgtgctggcg gcgctcgtgc tggtgtggtt
     6841 cgggcggggc ctggactggg cggaggtctc gggggcgata gggcgtgcgg actggcggat
     6901 ggtggcggcg ggcgtcgtga tcgtgtgggc gacgtatctg attcgcgcgt tccgctggcg
     6961 cacgctgctg aagccgctcg cgccgcgggc ttccttgcgc gagacgttcg gggcgacgac
     7021 cgtaggcttc gccgcgatct tcctcgtcgg tcgcgccgtg ggcgaggtgc tgcggcccgc
     7081 cttcctcgcc gtacgcgacc cggaggtgaa gcccggcccg gcgttcgtca ccatcgcggt
     7141 cgagcgcatc tacgactcgg tggcgatggt cgtgctgttc gcggcgaacc tgctggtgtt
     7201 cagaccggcg acggctgacg cggctggttt gcagcggctc cggctgatcg gcctcgtgct
     7261 gctcgcgggg gcggtggtcg gggtggccgc gctcgcgctg ttccggcggc acgcggggaa
     7321 ggtg
//
LOCUS       DBNGOH010000156         3263 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1123035, whole genome shotgun sequence.
ACCESSION   DBNGOH010000156 DBNGOH010000000
VERSION     DBNGOH010000156.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3263)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3263)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3263
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1123035"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..478
                     /locus_tag="AC159P_02840"
     CDS             <1..478
                     /locus_tag="AC159P_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HIR3694420.1"
                     /translation="VLLGRGSTVGQLYESITGGPLVFTWQGAVVAASVGALPLVVKTA
                     RAAFASVDAELEDAARTLGKSEWEVFRRVTLPLASRGVAAAAMLAFARALGDFGATLM
                     VAGNIPGRTQTAAVAIYDATQTGRDAHALTLVLILSAAAFAVLYTTNRLTATGHNR"
     gene            478..1587
                     /locus_tag="AC159P_02845"
     CDS             478..1587
                     /locus_tag="AC159P_02845"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein"
                     /protein_id="HIR3694421.1"
                     /translation="MLSARIRKQLAGVDDADGFTLDVDLAFPPGVTVLFGASGSGKTL
                     TLKSLAGLVRPDSGAIRIGDDTLFDSDRRIDLPIRARGAGYVFQHLALFPHLDALSNV
                     AFPLSHLPRAERSARALELLDKFHVGHTARRLPRHISGGEAQRVALARALAARPRFLL
                     LDEPLSALDEAVKLDIIADLKRTNRELRLPVVYVTHSRDEALALGERAYVYERGRVVA
                     AGEPSEVFRAPVNAGVARLTGVENIFEGVVADRDEAAGTMCVELAAGDGGTCRVEVPL
                     GRGGVGARVTLAVRSGDILLATERPRGLSARNLLAGTVSALEERGDQTLVRVRAGIEW
                     AASLTRQSVIELKLTEGKQVWLAIKTYSCRIFDGE"
     gene            complement(1680..2822)
                     /locus_tag="AC159P_02850"
     CDS             complement(1680..2822)
                     /locus_tag="AC159P_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012192350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - modified amino acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate-amine ligase"
                     /protein_id="HIR3694422.1"
                     /translation="MTPEKFTLGIEEEFQIVDPQTRELKSHIVEILEQGRMLLGEQVK
                     PEMIQSQVEVGTGVCANIQEARADITRLRSIVSWLARQKGLAIVAASTHPISHWKEQQ
                     ITDDPHYALLIEELQMVASSLLIFGLHVHVGLPDRERQIHVMNAARYFLPHVLALSTS
                     SPFWLGVNTGLKSYRCEVFKKFPRTDIPDHFGSYQSFQSYVELLLKTKSIQNPKKIWW
                     DVRPHPYFPTLEFRICDIPTRVDDTIAIAALFQAIVCKLDRLIEKNLGFRLYRRMLIQ
                     ENKWRAVRYGLDGKLIDFGKQTEVPARDLILELLEFVDDVLDDLDSRKEVEHIHTILQ
                     RGTSADEQLRVFNETGSLQSVVDTLIESTIENVPADLQNTTAAPTS"
     gene            complement(2877..3209)
                     /locus_tag="AC159P_02855"
     CDS             complement(2877..3209)
                     /locus_tag="AC159P_02855"
                     /inference="COORDINATES: protein motif:HMM:NF018894.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HIR3694423.1"
                     /translation="MNFLNSQTPQDERRQYGRSRLIIDVFFNGEDVTGVAGTKDISPG
                     GLYMNTKTEIPEGSLLTLRVPVAGRDIVCNAEVIYSNPGRGVGVRFQGMPEEDRAFLE
                     QELAGEAG"
BASE COUNT          483 a         1088 c         1109 g          583 t
ORIGIN      
        1 cgtgctgctc gggcgcggca gcaccgtcgg ccagctctac gaatcgatca cgggcgggcc
       61 gctcgtcttc acctggcagg gcgcggtcgt cgccgcgtcg gtcggcgcgc tcccgctcgt
      121 cgtgaagacg gcgcgcgccg cgttcgccag cgtggacgcg gaactcgaag acgccgcgcg
      181 cacgttgggt aagtcggagt gggaagtctt ccggcgcgtg acgctgccgc tcgcctcgcg
      241 cggggtggcg gcggcggcga tgctcgcttt cgcgcgcgcg ctcggcgact tcggcgcgac
      301 gctgatggtc gcgggcaaca tccctggccg cacgcagacc gcggcggtcg ccatctacga
      361 cgctacgcag acgggccgcg acgcacacgc gctcacgctc gtcctcatcc tctccgcagc
      421 cgctttcgcc gtcctctaca ccaccaaccg gctcaccgcc acgggccaca acaggtgatg
      481 ctctccgcgc ggatcagaaa gcagctcgcc ggtgttgacg acgcggacgg gttcacgctc
      541 gacgttgacc tcgccttccc gcccggcgtg accgtgctct tcggcgcctc ggggtctggg
      601 aagaccctga cgctcaagtc gctcgccggc ctcgtccgcc ccgactcggg cgccatcagg
      661 atcggcgacg acaccctctt cgattccgac cgacgcatcg accttcccat ccgcgcgcgc
      721 ggcgcgggct acgtcttcca acacctcgcc ctcttcccac acctcgacgc gctctcgaac
      781 gtcgccttcc ccctctcgca cctgccgcgc gccgaacgca gcgcgcgtgc gctcgaactg
      841 ctcgacaagt tccacgtcgg gcacaccgcg cgtcgtctgc cgcggcacat ctccggcggc
      901 gaggcgcagc gcgtcgccct cgcgcgcgcg ctggccgccc gtccgcgctt cctgctgctc
      961 gacgagccgc tctccgcgct cgacgaagcg gtcaagctcg acatcatcgc agacctcaag
     1021 cggacgaacc gcgagctgcg gctgcccgtc gtctacgtga cgcacagccg cgacgaggcg
     1081 ctggcgctcg gcgagcgcgc ctacgtctac gagcgcgggc gcgtcgtggc cgcgggcgag
     1141 ccgtcggaag tcttccgcgc gcccgtcaat gccggcgtcg cgcgcctcac cggcgtcgag
     1201 aacatcttcg aaggcgtggt ggccgacagg gacgaagcgg cgggcacgat gtgcgtcgaa
     1261 ctcgccgcgg gcgacggcgg cacctgtcgc gtcgaagtgc cgctcggtcg cggcggcgtc
     1321 ggcgcgcgcg tcaccctcgc ggttcgttcc ggcgacatcc ttctcgccac cgagcgcccc
     1381 cgcggactct ccgcgcgcaa cctcctcgcg ggcacggtga gcgcgctcga agagcgcggc
     1441 gaccagacgc ttgtgcgcgt ccgcgccggc atcgagtggg ccgcgagcct cacgcgccaa
     1501 tcggtaatcg aattgaagct gacggaaggc aaacaggtgt ggctcgccat caaaacctac
     1561 tcctgccgga tattcgacgg cgaataaaaa gggaaaaggg gaagagtgga aaaggcgaaa
     1621 tggaagacga gagaattgct gctcctccct tttcgcctct tccccttttc ccccttctgt
     1681 tagctcgtcg gcgcagccgt ggtgttttgc aagtcggcgg gaacgttctc gatggtgctc
     1741 tcgatgagtg tatcgacgac ggattggagg ctgccggtct cgttgaagac gcggagctgt
     1801 tcgtcggcgg aggtaccgcg ctggaggatg gtgtggatgt gctcgacctc cttgcgcgag
     1861 tcgaggtcgt cgagcacgtc gtccacgaat tcgaggagtt cgaggatgag gtcgcgtgcg
     1921 ggcacctcgg tctgcttgcc gaagtcgatg agcttgccgt cgaggccgta gcgcacggcg
     1981 cgccacttgt tctcctggat gagcatgcgg cggtagaggc ggaagccgag gttcttctcg
     2041 atgaggcggt cgagcttgca gacgatggcc tggaagagcg cggcgatggc gatggtgtcg
     2101 tccacgcgcg tcgggatgtc gcagatgcgg aactccagcg tcgggaagta ggggtgcggg
     2161 cgcacgtccc accagatttt cttggggttc tggatgctct tggtcttcaa gagcagctcg
     2221 acgtagctct ggaacgactg gtatgagccg aagtggtcgg ggatgtccgt gcgcgggaac
     2281 ttcttgaaga cttcgcagcg gtaggacttc agtcccgtgt tgacgccgag ccagaagggc
     2341 gaagaggtgg agagcgccag gacgtgcggg aggaagtagc gcgcggcgtt catgacgtga
     2401 atctggcgct cgcggtcggg cagcccgacg tggacgtgga ggccgaagat gagcagggag
     2461 ctggcgacca tctgtagctc ttcgatgagg agcgcgtagt gcgggtcgtc ggtgatctgc
     2521 tgctccttcc agtgcgagat cgggtgcgtc gaggcggcga cgatggcgag gcccttctgc
     2581 cgcgcgagcc acgagacgat ggagcgcagg cgcgtgatgt cggcgcgcgc ctcctggatg
     2641 ttggcgcaga cgccggtgcc cacctcgacc tgcgactgga tcatctcggg cttgacctgc
     2701 tcgccgagga gcatccggcc ctgctcgaga atctcgacga tgtgcgactt cagctcgcgc
     2761 gtctgcgggt ccacgatctg gaactcttcc tcgatgccga gggtgaattt ctccggcgtc
     2821 atacccttct ctcctcgccc gaatgtaccc ctactgcgtt ctgtttgcgc tgtgccttaa
     2881 ccggcctcgc ccgccagctc ctgttcgagg aacgcacgat cctcttccgg catcccctgg
     2941 aacctgacgc cgacgccgcg accggggttg ctgtagatga cctcggcgtt gcagacgatg
     3001 tcgcgccccg cgacggggac gcgcagcgtg agcagcgagc cttcggggat ttcggtcttc
     3061 gtgttcatgt agaggccgcc ggggctgatg tccttcgtcc cggcgacccc cgtcacgtcc
     3121 tcgccgttga agaacacgtc gatgatgagc cgcgagcgcc cgtactgtcg gcgctcgtcc
     3181 tgcggcgtct ggctgttgag gaagttcatg gcgcgtctcc gcggctgccc gcttcagctt
     3241 ttctcgccga tgatgaaggt cgg
//
LOCUS       DBNGOH010000157         4056 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_939660, whole genome shotgun sequence.
ACCESSION   DBNGOH010000157 DBNGOH010000000
VERSION     DBNGOH010000157.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4056)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4056)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4056
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_939660"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..245)
                     /locus_tag="AC159P_02860"
     CDS             complement(<1..245)
                     /locus_tag="AC159P_02860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694424.1"
                     /translation="MRSRSKRKAAAPGVALARRARTLFSVLLLGTTLLNPAAIIFAQD
                     QDTSISRLREQVAAMKKVDEDAGASGEVKRVNRAFLD"
     gene            complement(311..679)
                     /locus_tag="AC159P_02865"
     CDS             complement(311..679)
                     /locus_tag="AC159P_02865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694425.1"
                     /translation="MRRQNIGVFIVAPGEIVTVEVEAVEVGNFETFTVDEELLDPLPG
                     VSPPTYQFPVTVDVCFAHFAHVSGFFPEGTPDTARYEVFVSGSEGGERLRSHVIRKTH
                     DLDSRDIEFRCRQEGVNCDE"
     gene            1059..2222
                     /locus_tag="AC159P_02870"
     CDS             1059..2222
                     /locus_tag="AC159P_02870"
                     /EC_number="1.17.98.2"
                     /inference="COORDINATES: protein motif:HMM:NF015983.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HIR3694426.1"
                     /translation="MDYTTRELLKRYASLALGQPFAPVLLNILVTSVCDMRCTHCFFT
                     DELDDRPRKKLQMKTHEIERVSETLGGNLGVLILAGGEPFTRKDLPEIVRAFYENNKL
                     ESVYLMSNGQIQKRIIPDVTRILEECPNLNVAVALGIDGLKAQHEKIRQKPGSWDIAI
                     DTARQLQAIKREYRRLDIQTCTCFMNTNQDTIFEWYDFLKYDLRPDKVNFNYIRPPSA
                     DPKELDIDHARYSKLARMIDDDSRHAAIKNNYGGEAGFFKAAIDIYMHGLIDRTQQEQ
                     KAQLTCYAGTAGAVIYDEGTVSSCENLDAVGNLREHEWNFQSLWRSEAMKARRKKAAD
                     GCFCTHESNCYYPSLPFNPKHLIKIKKLEREMKKASRAHGKQGEEADGVAVKA"
     gene            2300..3487
                     /locus_tag="AC159P_02875"
     CDS             2300..3487
                     /locus_tag="AC159P_02875"
                     /inference="COORDINATES: protein motif:HMM:NF018613.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009247 - glycolipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGDG synthase family glycosyltransferase"
                     /protein_id="HIR3694427.1"
                     /translation="MPKILIISSDTGGGHRSAAAAIVAGVQKFVKSDSCAVRVVRAVE
                     ESHHLSEKLVRLYNWVLRNRQHWMKYLYWCVNRFRPETREFFHRRCIGFCRELFEKWC
                     PHVVVSVHPLTQHIFGRVLKELGLSERIPLVSVVTDPCYGFWKGWACDDVTLYLVASE
                     EARQQLIDYGVKPERIKVSGMPVHPKFELPDERAAQAARRALGLDPEKFTVFVNAGWV
                     GGGNIPQIFGELVRGDLNVQAIFLAGRNEELRAEAETLATSARFPVKVIGYSEQVEQL
                     MQAADVMISKLGGLTTFEALACRVPIIADAITPPMPQESGAAKMLAKRGAGVLLNRAS
                     DVVPVVRRMVEDSRYYATLRAATTSLAIPDATRRIVEEITALLPATNFAEEENLKAEV
                     RSA"
     gene            3705..>4056
                     /locus_tag="AC159P_02880"
     CDS             3705..>4056
                     /locus_tag="AC159P_02880"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3694428.1"
                     /translation="MSLLLEGKRAFITGGTRGIGAALCEVFAREGADVAFVYHSRDDL
                     AEETAAKIRAHGRRPLAHKISVTDRLGLKKLTREIVEAWGGIDVLVNNAAINRGDNFA
                     TTTDRAWDEIINTNV"
BASE COUNT          756 a         1353 c         1255 g          692 t
ORIGIN      
        1 tcgtcgagga acgcgcggtt gacgcgcttg acctcgccgc tcgcgccggc gtcctcgtcc
       61 actttcttca tcgccgcaac ctgctctcgg agacggctga tggatgtgtc ctgatcctgc
      121 gcgaagatga tcgccgccgg gttcaggagc gtcgtcccca gcagcaggac gctgaagagc
      181 gtccgcgcgc gcctcgcgag ggcgacgccc ggcgcggcgg cttttcgttt gctacggctg
      241 cgcatgtctg atgcctcctt actattcggt aaaggttgaa ttgcccgatc ctcgttgccg
      301 tcctcgtccc ctactcgtcg caattcactc cctcctgccg gcacctgaac tcgatgtcgc
      361 ggctgtcgag gtcgtgcgtc ttgcggatga cgtggctcct taatctctct ccgccctcgc
      421 tgccggagac gaagacttcg tacctcgcgg tatcgggcgt cccctccggg aagaagccgc
      481 tgacgtgcgc gaagtgggcg aagcaaacgt ccacggtgac ggggaattgg taggtcgggg
      541 ggctgacacc cggcagcggg tctagcaact cctcatcgac cgtgaaggtc tcgaaattcc
      601 cgacctcgac ggcctcgacc tcgaccgtca ctatctcgcc gggggctacg atgaaaactc
      661 cgatgttctg tcttctcatg accgtgtcgt ctccgtggct ggaaatgttg acgtgacact
      721 cggctgctcg cgccgccgaa gcctgcctgt cgggcggatt gctccccgcc ggctgctgga
      781 gcaggatggg taagggctgg gcgccgcggc gtcgccgcgg gccggaatct gtcctgtcac
      841 ccctatatga agggcgcgcg ctaaacgttt cgccccgcgc gaataattgt accgacacac
      901 ggcgcgcggc tgcatcaaag gttggcgcgc gcgtgaggcc ggtcgcgttg acacccggcg
      961 ggaaatgccg tatcagtaga tggtctgcgc tattttggcg ggacggtgag gttggcgcgc
     1021 ggcgctcggg ctcgcacgcc ggaaagtatg agtttcgaat ggattatacg accagggaac
     1081 ttctgaagcg ttacgccagt ctggcgctgg ggcagccgtt cgcgccggtg ttgctgaaca
     1141 ttttggtgac gagcgtgtgc gacatgcgtt gcacgcactg cttcttcacg gacgagctgg
     1201 acgaccgccc gcgcaagaag ttgcagatga agacgcacga gatcgagcgc gtctcggaga
     1261 ctttgggcgg caacctcggc gtgctcatcc tcgccggggg cgagcctttc acgcgcaaag
     1321 atttgcccga gatcgtccgc gcgttctacg agaacaacaa gctcgaatcg gtctacctga
     1381 tgtcgaacgg gcagattcag aagcgcatca tccccgatgt cacacgtatc ctcgaagagt
     1441 gcccgaacct gaacgtggcg gtcgcgctcg ggatcgacgg gctgaaggcg cagcacgaga
     1501 agattcggca gaagcccggc tcgtgggaca tcgccatcga cacggcgcgg caactgcaag
     1561 ccatcaagcg cgagtaccgg cggctcgaca tccagacctg cacctgcttc atgaacacga
     1621 accaggacac gatcttcgag tggtacgact tcctcaagta cgacctcagg cccgacaagg
     1681 tcaacttcaa ctacatccgc ccgccgtcag ccgacccgaa agagctggac atcgaccacg
     1741 cgcgctactc caagctcgcg cggatgatcg acgacgactc gcgccacgcc gccatcaaga
     1801 acaactacgg cggcgaggcc ggcttcttca aggccgccat cgacatctac atgcacggcc
     1861 tgatcgacag gacccagcag gagcagaagg cgcagctcac gtgctacgcc ggcaccgccg
     1921 gcgcggtcat ctacgacgag ggcaccgtct cctcgtgcga aaacctcgac gccgtcggga
     1981 acctgcgcga acacgagtgg aacttccagt cgctgtggcg ctcggaggcg atgaaggcgc
     2041 gacgcaagaa ggcggcggac ggctgcttct gcacgcacga gtcgaactgc tactatccgt
     2101 cgctgccgtt caacccgaag cacctgatta agatcaagaa actcgaacgc gagatgaaga
     2161 aggcgagccg cgcgcacggc aagcagggcg aagaggccga cggcgtggcg gtcaaggctt
     2221 gaagaatttt cgggccgccc aagctccgac cgaagaccga agaccgaaag ctgaaaactc
     2281 atccatgtcc cccgaccgtg tgccgaaaat cctgatcatc tcgtccgaca cgggcggcgg
     2341 ccaccgctcg gcggcggcgg ccatcgtcgc cggggtgcag aagttcgtca agagcgactc
     2401 gtgcgcggtg cgcgtcgtgc gcgcggtcga ggagtcgcac cacctatcgg aaaagctcgt
     2461 ccgcctctac aactgggtgc tgcgcaaccg gcagcactgg atgaagtatc tctactggtg
     2521 cgtcaaccgc ttccgccccg agacgcgcga gttcttccac cgccgctgca tcggcttctg
     2581 tcgcgagctg ttcgagaagt ggtgcccgca cgtcgtcgtc agcgtccacc cgctgacgca
     2641 gcacattttc ggtcgcgtcc tcaaggaact cggcctcagc gaacgcatcc cgctcgtcag
     2701 cgtcgtcacg gacccctgct acgggttctg gaagggctgg gcctgcgacg acgtgacgct
     2761 ctacctcgtg gcgagcgagg aggcgcgcca gcagctcatc gactacggcg tcaaacccga
     2821 gcgcatcaag gtctcgggga tgcccgtcca cccgaagttc gagctgccgg acgagcgggc
     2881 cgcgcaggcc gcgcgccgcg ccctcggcct cgaccccgag aagttcaccg tcttcgtcaa
     2941 cgccggctgg gtcggcggcg gcaacatccc gcaaatcttc ggcgagctgg tgcgcggcga
     3001 cctgaacgtg caggccatct tcctcgccgg caggaacgag gagctgcgcg cagaggccga
     3061 gacgctcgcc acgagcgcgc gcttcccggt caaggtcatc ggctactccg agcaggtcga
     3121 gcaactgatg caggcggcgg acgtgatgat ctcgaagctc ggcggtctga cgaccttcga
     3181 ggcgctcgcc tgccgcgtcc cgatcatcgc cgacgccatc acgccgccca tgccgcagga
     3241 gtccggcgcc gccaaaatgc tcgccaaacg cggcgcgggt gtcctgctca accgcgccag
     3301 cgacgtggtg cccgtcgtgc gccgcatggt cgaggactcg cgctactacg ccactttgcg
     3361 cgccgccacc acgagcctcg ccatccccga cgccacacgc cgcatcgtcg aagagatcac
     3421 cgcgctcctc cccgcgacca acttcgccga agaagaaaac ctcaaggccg aagttcgctc
     3481 cgcctgaaac gaaaggactg aggactgagg actgaggact gagtgagtga caggttattt
     3541 tcactcagtc ctcagtcctc agtcctcagt cctcagtcct ttcacgtttg acgcgcctcc
     3601 gcgtttaagc tttaatcgga ggcgttctat tcatcaagac aacccgcctt cgcgtttcgt
     3661 ccgtcgttga gcggaggcgt gtgcgaatcg gggacgagcg taccttgagt ctcttgctgg
     3721 aagggaagcg cgccttcatc accggcggca cgcgcggcat cggcgccgcg ctctgcgagg
     3781 tcttcgcgcg cgagggcgcc gacgtggcct tcgtctatca ctcgcgcgac gatctggccg
     3841 aagagaccgc cgccaaaatc cgggcgcacg gtcgccgccc gctggcccac aaaatctccg
     3901 tcaccgaccg cctcggcctg aagaagctca cgcgcgagat cgtcgaggcg tggggcggca
     3961 tcgacgtgct cgtcaacaac gcggccatca accgcggcga taacttcgcc accaccaccg
     4021 atcgcgcctg ggacgagatc atcaacacca acgtcc
//
LOCUS       DBNGOH010000158         3276 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4788647, whole genome shotgun sequence.
ACCESSION   DBNGOH010000158 DBNGOH010000000
VERSION     DBNGOH010000158.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3276)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3276)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3276
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4788647"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(168..443)
                     /locus_tag="AC159P_02885"
     CDS             complement(168..443)
                     /locus_tag="AC159P_02885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694429.1"
                     /translation="MADKDKEKMVDTGGDLSPTGVNPRDMGDDPTSSMAASASASAAR
                     GRDNLGGGHGDGIKGVSHTGEMSTRSDTTAGSRSAIDTDDDEERGGR"
     gene            714..1853
                     /gene="hemW"
                     /locus_tag="AC159P_02890"
     CDS             714..1853
                     /gene="hemW"
                     /locus_tag="AC159P_02890"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HIR3694430.1"
                     /translation="MERAGVYIHIPFCRARCSYCDFATGAYDGALAAAYVRALAQEIE
                     AFDGASPRAEVDTVYFGGGTPSLLTAAQLAHVLAAVRRRFAVEADAEVTMEMNPGTLS
                     PHVLREFRAAGVNRASFGAQTFDDRELKRLGRTHTADDARRTLSMLGDAGFDNVSFDL
                     IAGLPEQTVAAWSRNLDEALALGPRHLSLYLLEVHEGTPLAEQLRQGRFRAPDPEAAA
                     EMYELLVERTRAAGYEQYEISNFCLPGYEARHNTKYWTGAPYYGFGSSAHSFDGSARR
                     WSNERDARRYVELIEAGSSAVVERTRLDAHEASAEAIFLGLRLLRRGVNLAEHRARFR
                     RDVRREHAADLARFREAGLVEIDGDVLRLTPHGALLSNEVFAAFV"
     gene            1862..2149
                     /locus_tag="AC159P_02895"
     CDS             1862..2149
                     /locus_tag="AC159P_02895"
                     /inference="COORDINATES: protein motif:HMM:NF013500.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HIR3694431.1"
                     /translation="MKEIILSGAGWRTDEDFYNAFLEAVGAPGWHGHNLDALYDSISC
                     DDINKLETPYRVRISGVSSMSAEARQMVESFEKLVAEMRKEGIEVSVVCEP"
     gene            2183..3232
                     /locus_tag="AC159P_02900"
     CDS             2183..3232
                     /locus_tag="AC159P_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011196526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004793 - threonine aldolase activity
                     [Evidence IEA];
                     GO_process: GO:0006567 - L-threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine aldolase family protein"
                     /protein_id="HIR3694432.1"
                     /translation="MTLIDLRSDTVTKPTPAMRRAMAEAEVGDDVYGEDPTVNRLQER
                     AAELFEKEAAIFVPSGSMGNQIAVKLHTRPGSEVVIEERGHIYNYEMAAMAAVSGALA
                     RPVKSSDGTGVLAWDDIAGALHVGGAYYVAPTALVALENSHNLAGGTLMTRARTEEMC
                     ARAHAAGLAVHLDGARVFNAAAALGERVAALARPVDSVMFCLSKGLGAPVGSMLLGTR
                     AFIEEARAWRKLLGGGMRQAGVLAAAGLVALEESPPRLVEDHRNARRLAEGVAELPGF
                     RIDPEAVQTNIVIFDVTGTGMSSAEICARLQEEHGVLAHGFGDGIRMVTHYDVSGADI
                     EHALVALKKVLRTED"
BASE COUNT          530 a         1134 c         1090 g          522 t
ORIGIN      
        1 tgttgcctga attggtcgcc gcagacgtgt acgacagcgg aaggtgagta aaaaaagcgc
       61 gcggctccgg gctttaggtt gaaccggagt cgggcgcttt tccctcgccg gagcgagaag
      121 cctgcaagtc gccggggcga gagtgtgtct cttacgcccg gcgtgtgtta tctgcctccg
      181 cgttcttcgt cgtcgtcggt gtcgatggcc gagcggctgc ccgcggtggt gtcgctgcgg
      241 gtgctcatct cgccggtgtg gctgacgccc ttgatgccgt cgccgtggcc gccgccgagg
      301 ttgtccctgc cgcgcgcggc gctggcgctg gcgctcgcgg ccatgctgct ggtcgggtcg
      361 tcacccatgt cgcgcgggtt gacgcccgtg gggctgaggt cgccgcccgt gtccaccatc
      421 ttctctttgt ccttgtctgc catctcgttc gtcctccaag gttgatgccg gcctttatct
      481 gttcggcaag aaacagcctg aagagattca aagaccatgc cagaagccgg cgcgtgaacg
      541 cacgacccga actcgcagtg attgtttggg gatgcgggcc gtgcggcgcg cgacggcgcg
      601 cagcacaggc ggctttcgtc aaaccgaagt cgtacaccat cgcgaaaaaa ccgtacacgg
      661 cgccccgccg ccgccgcgtg gtatactcgg cgacacttag ggatacctga acaatggaac
      721 gggccggagt ctacatccac atccccttct gccgcgcgcg ctgctcctac tgcgacttcg
      781 ccacgggggc ttacgacggc gcgctcgccg ccgcttacgt ccgcgctttg gcgcaagaga
      841 tcgaggcgtt cgacggcgcg tcgccgcgcg cggaggttga caccgtctac ttcggcggcg
      901 ggacgccgag cctgctgacg gcggcgcagc tcgcgcacgt cctcgcagcc gtccgccgcc
      961 gcttcgccgt cgaggccgac gccgaagtga cgatggagat gaaccccggc acgctctcgc
     1021 cgcacgtctt gcgcgagttc cgcgcggcgg gcgtgaaccg tgcgagcttc ggcgcgcaga
     1081 ccttcgacga ccgcgagctg aaacggctgg gccgcacgca cacggcggac gacgcgcgcc
     1141 gcacgctctc gatgctcggc gacgcggggt tcgacaacgt cagcttcgac ctcatcgcag
     1201 ggctgcccga acagacggtc gccgcctggt cgcgcaacct cgacgaggcg ctcgcgctcg
     1261 ggccgcggca cctctcgctc tacctcctcg aagtccacga agggacgccg ctcgccgaac
     1321 agctcaggca ggggcgcttc cgcgcgcccg accccgaagc cgccgcggag atgtacgagc
     1381 tgctggtcga gcgcacgcgc gccgcgggct acgagcagta cgagatttcc aacttctgcc
     1441 tgccgggtta cgaagcgcgg cacaacacga aatactggac gggcgcgccc tactacggct
     1501 tcggcagctc cgcgcactct ttcgacggga gcgctcggcg ctggtcgaac gagcgcgacg
     1561 cgcgccgcta cgtcgagctg atcgaggccg gcagcagcgc cgtcgtcgag cgcacccggc
     1621 tcgacgcgca cgaggcgagc gccgaagcga tcttcctcgg cctccggctg ctgcgccgcg
     1681 gcgtcaacct cgccgagcac cgcgcacgct tccggcgcga cgtgcgccgc gagcacgccg
     1741 cagacctcgc gcgcttccgc gaggcgggcc tcgtcgagat cgacggcgac gtgctgcgcc
     1801 tgactccgca cggcgcgttg ctctccaacg aagtcttcgc tgctttcgtt taaccacgac
     1861 gatgaaagag atcatactgt cgggcgcagg ttggcggacg gatgaggatt tttataacgc
     1921 tttcctcgaa gccgtcggcg cccccggctg gcacggacac aacctcgacg ccctgtacga
     1981 cagcatcagt tgtgatgaca tcaacaagtt ggaaactcct taccgggtga gaatcagcgg
     2041 cgtctcgtcg atgtctgccg aggcgcggca gatggtcgag agtttcgaaa aactcgtcgc
     2101 cgagatgcgg aaagaaggca tcgaggtgag cgtcgtgtgc gagccgtagt catctttcag
     2161 cggacaaccc ttcaacgagc aaatgacctt gatcgacctg cgcagcgaca ccgtgacgaa
     2221 gccgacgccc gcgatgcgcc gcgcgatggc cgaggccgag gtgggcgacg acgtgtacgg
     2281 cgaagacccg accgtcaacc gcctgcaaga gcgcgccgcc gagctgttcg agaaggaggc
     2341 cgccatcttc gtgccctccg gctcgatggg caaccagatc gccgtcaagc tccacacgcg
     2401 gcccggcagc gaggtcgtca tcgaagagcg cggccatatc tacaattacg agatggcggc
     2461 gatggccgcc gtctcgggcg cgctcgcgcg gcccgtcaaa agctcggacg ggacgggcgt
     2521 cctcgcgtgg gacgacatcg cgggcgcgct ccacgtgggc ggcgcctact acgtcgcgcc
     2581 gaccgcgctc gtcgcgctgg agaactcgca caacctcgcg ggcggaacct tgatgacgcg
     2641 cgcgcgcacc gaagagatgt gcgcgcgcgc gcacgcggcg ggcctcgccg tccacctcga
     2701 cggcgcgcgc gtcttcaacg ccgcggcggc gctcggcgag agggtcgccg cgctcgcgcg
     2761 ccccgtcgat tccgtcatgt tctgcctgtc gaagggactc ggcgcgcccg tcggctcgat
     2821 gctgctcggc acgcgcgcct tcatcgagga ggcgcgcgcg tggcgcaagc tcttgggcgg
     2881 cgggatgcgg caggcgggcg tgctcgccgc cgccggactg gtcgcgctcg aagagtcgcc
     2941 gccgcgcctc gtcgaagacc accgcaacgc gcgccgcctc gccgaaggcg tcgccgagct
     3001 gcccggcttc cgcatcgacc ccgaagccgt ccagaccaac atcgtcatct tcgacgtgac
     3061 cggcacgggg atgtcgtccg ccgaaatctg cgcgcgcctt caggaagaac acggcgtcct
     3121 cgcccacggc ttcggcgacg gcatccgcat ggtcacgcac tacgacgtgt ccggcgcaga
     3181 catcgagcac gcgctcgtcg cgctcaaaaa agtactgagg actgaggact gaggactgag
     3241 caaaatcgaa ttatctttgc tcagtcctca gtcctc
//
LOCUS       DBNGOH010000159         3183 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_390105, whole genome shotgun sequence.
ACCESSION   DBNGOH010000159 DBNGOH010000000
VERSION     DBNGOH010000159.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3183)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3183)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3183
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_390105"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..894)
                     /locus_tag="AC159P_02905"
     CDS             complement(<1..894)
                     /locus_tag="AC159P_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017690930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan lyase family protein"
                     /protein_id="HIR3694433.1"
                     /translation="MKLKTWSQAALVVGVMLALASGAQAQYLMENLGRGVVAVRQNET
                     QVYVGWRLLGTEYPADIRFNVYRAVGTGAPEQLNGDPLTETTDFTDTPPDFTEKLTYT
                     VRPVLNGVEQTDGTGSFVLPAGAPVRQYLSVPLQIPPPHVTPDGVSHIYRANDGTAAD
                     LDGDGEYEIILKWDPDNSGLGDTNATGWSSPTIVDAYKLDGTRLWRINLGVNVRSGSH
                     YTQLLAYDFDGDSKAELALKTADGTVDGQGHVIGNASADWRAPLGDPNWGRILTGPEY
                     LTVFNGLTGAEMATTNFIPGRD"
     gene            complement(1358..2470)
                     /locus_tag="AC159P_02910"
     CDS             complement(1358..2470)
                     /locus_tag="AC159P_02910"
                     /inference="COORDINATES: protein motif:HMM:NF025167.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HIR3694434.1"
                     /translation="MHSTPVQLGVRPRNRALWIGVLLSLLGGGAYGHEVQSSWTVVRF
                     GPDRCELNVRMHGETVRSLIQDEAPQATLEPENIDNIRPLLTAFANNLYEVSAGGQRL
                     TASLTDVTLVDDNLEFRLSYPRPGGGPLRLKALYLSRVAPDFIANVSVEGGAGQPPVN
                     KILNPREPSVEIEPPSGAAGHGAAADNPFLSFLKLGVEHILTGYDHLLFLLGLLVACR
                     RFKTMAVIVTCFTLAHSVTLALAALDVVSLPARVVEPLIAASIVFVGVENLVRRDEPR
                     WRWALTFAFGLIHGFGFAGVLREIGLGSTTSALVTSLFSFNLGVELGQLAVVALLLPL
                     LWKLRELPAYERYGRHAVSALVALTGAYWLAQRLFF"
     gene            complement(2604..3182)
                     /locus_tag="AC159P_02915"
     CDS             complement(2604..3182)
                     /locus_tag="AC159P_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR3694435.1"
                     /translation="MRESLELMIGFAGWRPETFASAREFLARPRVLTPNCLVLDVSLP
                     DLNGLDLQKLVADRTGMPVIFITGYGDIPMTVRAMRAGAVEFLTKPFDDEVLLGAIRH
                     AIECSTDALSSEAEIQALRDCYASLTTRERQVMALVVSGLLNKQVGLRLGISEITVKA
                     HRGRVMQKMKADSLADLVKTAERLRLAPANNP"
BASE COUNT          552 a         1014 c         1064 g          553 t
ORIGIN      
        1 gtcgcgcccc gggatgaagt tggtcgtcgc catctcggcg cccgtgaggc cgttgaagac
       61 cgtcaggtac tcagggccgg tgaggatgcg gccccagttg gggtcgccga ggggcgcgcg
      121 ccagtccgcg ctcgcgttgc cgatgacgtg gccctgcccg tccaccgtcc cgtcggcggt
      181 cttcagagcc agctcggcct tgctgtcgcc gtcgaagtcg taggcgagga gctgcgtgta
      241 gtggctgccc gagcggacgt tgacgccgag gttgatccgc cagaggcgcg tgccgtcgag
      301 cttgtaggca tcgacgatgg tcgggctgct ccagcccgtc gcgttcgtgt cgccgaggcc
      361 ggagttgtcc gggtcccact tgagaatgat ctcgtattcg ccgtcgccgt cgaggtctgc
      421 ggcggtgccg tcgttggcgc ggtagatgtg gctcacgccg tcgggcgtca cgtgcggcgg
      481 ggggatttgc agcggcaccg agaggtactg gcgcacgggc gcgcccgccg gcagcacgaa
      541 ggagcctgtg ccgtccgtct gctcgacgcc gttaagcacg ggccgcacgg tgtatgtgag
      601 cttctcggta aagtccggcg gagtgtccgt gaagtcggtc gtctcggtca gcgggtcgcc
      661 gttgagctgc tccggcgctc ctgtgccgac ggcgcggtag acgttgaaac ggatgtcggc
      721 agggtactcg gtgccgagca gtcgccagcc gacgtagacc tgcgtctcgt tctggcgcac
      781 ggcgaccacg ccgcgcccca ggttctccat caggtactgc gcctgcgcgc ccgatgccag
      841 cgccagcatc acaccgacca ccagcgccgc ctgcgaccac gttttcaact tcatcatgct
      901 ccttcagcct ttcgtccagt acttcgtcac gacaggattt cgccccgcgg tcaaaactct
      961 gcgcttatct tgtccttggg aaatgatgac gacgcgtgag gggggctccg tgtgcgtccc
     1021 cgtagattca gtagcgaaat cgtcgttgcc cgcccgaggt gtgcgccaca aattacttct
     1081 tgaacacgca cggcacgtca gaggcgacgg cggcgcggcg atgccgggtt tgattgctat
     1141 cgagaattct agccgcgaag ggccgcggaa gtcttgggct gtttggacga atttcttcgc
     1201 acattcggca gcaggaaaga aagggggaaa aggggaagag gggaaagggc gaaaagggaa
     1261 gacgacaggc acgatgcttc tcatcatcga ttcgatgaca ggcacggtgc ttctcgtcct
     1321 tccttttccc cacttcccct tttaaccttt tcgcccttca gaagaagagt ctttgcgcga
     1381 gccagtaggc gcccgtgagc gcgacgaggg cggagacggc gtgtctgccg tagcgctcgt
     1441 aggcgggcag ctcgcgcagc ttccagagca gcggcagcag gagcgccacg accgcgagct
     1501 gccccagctc gacgccgagg ttgaacgaga agagcgacgt gaccagcgcc gaggtcgtcg
     1561 agccgaggcc gatctccctg agcacgcccg cgaagccgaa gccgtgaatc agcccgaacg
     1621 cgaacgtcaa agcccaacgc caccgcggct cgtcgcgccg caccagattt tctaccccga
     1681 cgaagacaat cgaggccgcg atcaaaggct cgacgacccg cgcggggaga ctcaccacgt
     1741 cgagcgccgc gagcgccagc gtgaccgagt gggcgagcgt gaagcaggtg acgatgacgg
     1801 ccatcgtctt gaaacgccgg cacgcgacca gcaggccgag caggaagagc aggtggtcgt
     1861 agcccgtgag gatgtgctcg acgccgagct tgaggaagga gaggaacgga ttgtcagccg
     1921 cggcgccgtg tcccgccgcg cccgacggcg gttcgatctc cacggacggc tcccgtgggt
     1981 tcagaatttt attgaccggc ggctgacccg ccccgccctc gacgctcacg ttggcgatga
     2041 agtccggcgc gacccgcgaa aggtacaacg ccttcaggcg cagcgggccg ccgccgggcc
     2101 gcgggtagga cagacggaat tcgaggttgt catccaccag cgtcacgtcc gtaagcgacg
     2161 ccgtcaggcg ctgcccgccc gcgctcacct cgtaaaggtt attggcgaac gccgtgagga
     2221 gcggtcggat gttgtcgatg ttctccggtt cgagcgtcgc ctgcggcgcc tcgtcctgta
     2281 tcagcgagcg caccgtctcg ccgtgcatcc tgacgttcag ctcgcacctg tcggggccga
     2341 acctcaccac cgtccagctc gactgcacct cgtggccata ggcgccgccg ccgagcagag
     2401 acaggagcac gccgatccaa agcgccctgt tccgggggcg cacgccgagc tgaacgggtg
     2461 tgctgtgcat aaagtcgaaa gccgtgggcg gcgcgaacga gcgggaaaga cggtcatgcc
     2521 ctacgaccac gggcgggagg attcagaccg gtcggacgta aacttcctcg gctactccga
     2581 atccgagtaa cccttgtgtc gccttatgga ttattcgcgg gtgcaaggcg gagtctctca
     2641 gccgttttca ccaggtcggc gagcgagtcg gccttcatct tttgcatcac cctgccgcgg
     2701 tgtgccttca ccgtgatctc gctgatcccg agcctcaaac cgacttgttt gttcaacaag
     2761 ccggagacca ccagcgccat gacctgccgt tcgcgggtgg tgagtgacgc gtaacagtcg
     2821 cggagcgcct gtatctccgc ctcgctggag agtgcgtcgg tgctgcactc gatggcgtga
     2881 cggatggcgc cgagcagcac ctcgtcgtcg aacggcttgg tcaggaactc gacggccccg
     2941 gccctcatgg cccgcaccgt catcgggatg tcgccgtatc ccgtgatgaa gatgacaggc
     3001 atgcctgtcc ggtcggcgac gagcttctgc aaatcgagac cgttgaggtc gggaagggaa
     3061 acgtcgagca cgaggcagtt aggcgtcagg acgcgcgggc gtgcgaggaa ttcccgcgcg
     3121 gacgcgaacg tctccggcct ccaccccgcg aagccgatca tcagttccag cgattcgcgc
     3181 acc
//
LOCUS       DBNGOH010000160         4934 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_756161, whole genome shotgun sequence.
ACCESSION   DBNGOH010000160 DBNGOH010000000
VERSION     DBNGOH010000160.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4934)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4934)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4934
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_756161"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            98..2560
                     /gene="purL"
                     /locus_tag="AC159P_02920"
     CDS             98..2560
                     /gene="purL"
                     /locus_tag="AC159P_02920"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF002290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HIR3694436.1"
                     /translation="MNPETITPEVVAAHNLTPEEFERIRELLGREPTFVELGIFSVMW
                     SEHCSYKSSRVHLKRLPTTGERVIVPPGENAGVVDVGDGWCAAFKVESHNHPSFIEPF
                     QGAATGVGGILRDIFTMGARPVAAMNSLRFGTLEHERHGRRNRALLAGVVAGIAHYGN
                     AFGVPTVGGEVAFDDAYALNPLVNAFALGLVRKDQIFFGTATGVGNPVLYVGAKTGRD
                     GIHGATMASAEFDDEALEKRPTVQVGDPFLEKLLLEACLEAMRSGAVAGIQDMGAAGL
                     TSSSCEMAARAGNGIELDLTLVPQREEGMSAYEILLSESQERMLIVAHRGRERQVVDI
                     FRRWDLDAVVIGRVTDTGNVVVLHHGERLADIPSKLLTDEAPRYERPMRAPEKTVESL
                     ASGVWRQKAETETQKAATGKEVNNVLVDENAGGEVENASIVNSDELDGILDAPSTITG
                     GSDSRLQTPDSRLDSNESLRRLLSATNVASKEWVYRQYDHMVRTNTAVLPGADAAVVR
                     IKETRRALAMCLDGNGRYCAVEPREGAKHLVAEAARNVVCVGARPIAITNCLNFASPE
                     RPEMMWAFSEVIDGMAEACRAFGTPVVSGNVSFYNETEGRGIHPTPVIGMVGLVEDVR
                     RVVTQGFKREGHFIALVGETGADLSVSEYAALVEGRTFEEMARANGLPRLDLERERAV
                     QETTLRAAEEGLLQSAHDCAEGGLAVALAEGCFSSLGRNHVAVEINLDGDLSPTAHLF
                     AETPSRIIVSFDETARPRLEQLAAEHGCPFRVIGRVGGTRLLINHNGAELISQDIAQL
                     ETLWRTSLGGKLRTEVLVAAAE"
     gene            complement(2626..3429)
                     /locus_tag="AC159P_02925"
     CDS             complement(2626..3429)
                     /locus_tag="AC159P_02925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694437.1"
                     /translation="MLARIAAALVLCLAAAAQASAQTDAAKFRHQPRKVEVGAVYHYL
                     KTNVDGTKPEHVSVYVAAPDRIESFKFHPQGTRAALVIATMDWSNFSAKRLESWQVFR
                     DDKKLFATLDYLPAERAVNVSIPPLGKPDEKTAIKHLPFHVYNFDLSSLNFAFRHLKD
                     PRATFTVGLADPTFKPDPPLFFYRGEVTVRYVGEETRNNVACRKYGIDGPGLSNRGGF
                     IWVNKRGGHFEDIEIALPDNPEWQSFKFKLERTERMTREQWEAFMKAQF"
     gene            complement(3525..4187)
                     /locus_tag="AC159P_02930"
     CDS             complement(3525..4187)
                     /locus_tag="AC159P_02930"
                     /inference="COORDINATES: protein motif:HMM:NF016176.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HIR3694438.1"
                     /translation="MRLWEKISAFALCAALAHGAAGAVKTTGSTPAAAPQGATARYRL
                     DASRSTFNVRAFSGGVLWFKGHDHLVAARDFTGEATLTPGSVNPASLRLTVRAASLAE
                     TRDVFTEQQKRIIDKELREIVLEPDKYPEITFESTGVEAKAERGVFKVRLTGDLTLHG
                     VTRRVTIPADVTVAGDDLRARGAFHIERSDFKVKATSAFHGTVRVRDKLKVMFDIVAR
                     RI"
     gene            complement(4217..>4934)
                     /locus_tag="AC159P_02935"
     CDS             complement(4217..>4934)
                     /locus_tag="AC159P_02935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694439.1"
                     /translation="KRTDEVPYFVLRDGRLVLDDSFRTSRAFTLRQSAANRAGRWVRD
                     RVRVIQAVHQSHGAIKSAIAAWRSRRNASPEANDTKKTNGARGGEAKTTVLEEPGTDN
                     LIYREPAGEVWHEAWRVTEALIARMREETESRGAKFVAVTMSNAIQVHPHAAAREHFM
                     RRLGVETLLYPDLRLKALGEREGFTVINLAPELQLYAEQHNVFLHGFAPDIGNGHWNE
                     RGHAVAGELLARRLCESAPR"
BASE COUNT          794 a         1639 c         1665 g          836 t
ORIGIN      
        1 ggttcacggc tctttccgcg tttggagaaa ccggcgcgag cctttatcct gtgctgtcct
       61 acaggtcagc cgaacaaggt cgccgccgtt tcccgtcatg aaccctgaga ccattacccc
      121 ggaagtcgtc gccgcacaca atctcacccc ggaagagttc gagcgcatcc gcgagttgct
      181 cgggcgcgag ccgacgtttg tcgagctggg catcttctcc gtgatgtggt cggagcactg
      241 ctcttacaaa tcctcgcgcg tgcatctgaa gcgtctgccg acgacgggcg agcgcgtcat
      301 cgtgccgccg ggcgagaacg caggggtggt ggacgtgggc gacggctggt gcgccgcctt
      361 caaggtcgag tcgcacaacc acccgagttt tatcgagccg tttcagggcg cggccacggg
      421 cgtcggcggc atcctgcgcg acatcttcac gatgggcgcg cgccccgtcg ccgcgatgaa
      481 ctccttacgc ttcggcacgc tcgaacatga gaggcacggg cggcgcaacc gcgccctgct
      541 cgcgggcgtc gtcgccggga tagcgcatta cggtaacgcc ttcggcgtcc ccacggtcgg
      601 cggcgaggtc gccttcgacg acgcctacgc gctgaatcct ttggtcaacg ccttcgctct
      661 cggcctcgtc cgcaaggatc aaatcttctt cggcacggcc acgggcgtcg gcaaccccgt
      721 cctctacgtc ggcgcgaaaa cggggcgcga cggcatccac ggcgcgacga tggcctccgc
      781 cgagttcgac gacgaggcgc tggagaaacg ccccacggtg caggtcggcg acccgttcct
      841 cgaaaagctg ctcttggaag cctgcctcga agcgatgcgc agcggcgccg tcgccgggat
      901 tcaggacatg ggcgcggcgg gcctcacctc gtcctcgtgc gagatggccg cgcgcgcggg
      961 caacggcatc gagcttgatt tgactctcgt gccgcagcgc gaagagggca tgtccgctta
     1021 cgagatcctg ctctcggaat cgcaggagcg catgctcatc gtcgcgcacc gcgggcgcga
     1081 gcggcaggtc gtggacatct tccgccgctg ggacttggac gccgtcgtca tcgggcgcgt
     1141 gacggacacc ggcaacgtcg tcgtccttca tcacggcgaa aggctcgccg acatcccgag
     1201 caaactcctg accgacgagg cgccgcgcta cgagcgaccc atgcgcgcgc ccgaaaaaac
     1261 agtcgagagt ctggcgtctg gcgtctggcg tcagaaggca gaaaccgaaa ctcaaaaggc
     1321 cgcgaccgga aaggaagtga acaacgtgct cgttgacgag aacgcgggcg gcgaagtcga
     1381 gaacgcgtcc atcgtcaact cggacgaact cgacggcatc ctcgacgcgc cgagcacgat
     1441 cacgggcggc tctgactcca gactccagac tccagactcc agactcgatt caaacgagtc
     1501 cctgcgccgc ctgctctcgg cgacgaacgt cgcctcgaag gagtgggtct accggcagta
     1561 cgaccacatg gtgcggacga acacggcggt cttgccgggc gcggacgcgg cggtcgtgcg
     1621 gatcaaggag acgcgccgcg cgctcgccat gtgcctcgac ggcaacggtc gctactgcgc
     1681 cgtcgagccg cgcgagggcg cgaagcatct ggtggccgag gcggcgcgca acgtcgtctg
     1741 cgtcggcgcg cggcccatcg ccatcacgaa ctgcctgaac ttcgcctcgc ccgagcggcc
     1801 cgagatgatg tgggccttct ccgaagtcat cgacgggatg gccgaggcgt gccgcgcgtt
     1861 cgggacgccg gtcgtctcgg gcaatgtctc cttctacaac gagaccgaag ggcgcggcat
     1921 ccacccgacg cccgtcatcg gcatggtcgg cctcgtcgag gacgtgcgcc gcgtcgtcac
     1981 gcaaggcttc aagcgcgagg gccacttcat cgcgctcgtc ggcgagacgg gcgcagacct
     2041 ctccgtgagc gagtacgcgg cgctcgtcga agggcgcacg ttcgaagaga tggcgcgggc
     2101 gaacggtctg ccccgcctcg acctcgaacg cgagcgcgcc gtgcaggaga cgaccctgcg
     2161 cgccgccgaa gaggggctgc tccaatccgc gcacgactgc gccgaaggcg gcctcgccgt
     2221 tgccctcgca gagggttgct tctcctcgct cggtcgcaac cacgtcgccg tcgaaataaa
     2281 tctcgacggc gacctctcgc cgaccgcgca cctcttcgcc gagacgccct cgcgcatcat
     2341 cgtcagcttc gacgaaacgg cccgcccgcg cctcgaacaa ctcgccgccg agcacggctg
     2401 ccccttccgc gtcatcgggc gcgtcggggg cacgcgactc ctcatcaacc acaacggcgc
     2461 cgaactcatc tcccaagaca tcgcccaact cgaaacgctc tggcgcacct cgctcggcgg
     2521 caagttgcga acggaagtcc tcgtcgccgc cgcggagtaa aaaagtactg agtactgagt
     2581 aaaagcaatc ggtctttcac tcagtactca gtactcagta cttttttaga actgcgcctt
     2641 catgaacgct tcccactgct cgcgcgtcat gcgctcggtg cgttcgagtt tgaatttgaa
     2701 gctctgccat tcggggttgt cggggagcgc gatctcgatg tcctcgaagt ggccgccgcg
     2761 cttgttgacc cagatgaagc cgccgcggtt cgagaggccg gggccgtcga tgccgtactt
     2821 gcggcaggcg acgttgttgc gcgtctcttc gccgacgtac ctgacggtca cttcgccgcg
     2881 gtagaagaag agcggcgggt cgggcttgaa ggtcgggtcg gcgaggccga cggtgaaggt
     2941 ggcgcgcggg tctttgaggt ggcggaaggc gaaattcaga ctcgacaagt cgaagttgta
     3001 gacgtggaag gggaggtgtt tgatggcggt cttctcgtcg ggtttgccga gcggggggat
     3061 ggagacgttg acggcgcgct ccgccggcag gtagtcgagc gtggcgaaaa gttttttgtc
     3121 gtcgcggaag acctgccacg attcgagccg cttcgccgag aagttcgacc agtccatcgt
     3181 ggcgatgacg agcgcggccc gtgtgccctg cgggtgaaac ttgaacgact cgatgcggtc
     3241 gggcgcggcc acgtagaccg agacgtgctc cggcttcgtc ccgtccacgt tcgtcttcaa
     3301 ataatgatag accgcgccca cttcgacctt gcgcggctgg tggcggaact tcgcggcgtc
     3361 ggtctgcgcg gacgcctgcg cggcggcggc gagacacagc acgagcgcgg cggcgattct
     3421 ggcgagcaaa gtttctctcc tcccgtagat gacctgcacc aagccgactg tacggagcgg
     3481 gccgcccgcc ggttcgccga aaatgttcgg cggcgcgcgc gccctcagat gcggcgcgcg
     3541 acgatgtcga acatcacttt gagcttgtcg cggacgcgca cagtgccgtg gaaggccgag
     3601 gtcgctttga ctttgaagtc gctgcgctcg atgtggaatg cgccgcgggc gcgcaagtcg
     3661 tcgccggcga ccgtcacgtc cgcggggatc gtgacgcggc gcgtcacgcc gtggagcgtg
     3721 aggtcgcccg tgagcctgac tttgaagacg cctcgctccg ccttcgcctc gacgccggtg
     3781 ctctcgaaag tgatctccgg atacttgtcc ggttcgagca cgatctcgcg cagctccttg
     3841 tcgatgatcc gcttctgctg ctcggtgaac acgtcgcgcg tctctgcgag cgaggcggcg
     3901 cgcaccgtca gccggagcga ggcggggttg accgagccgg gcgtgagcgt cgcctcgccc
     3961 gtgaagtcgc gcgccgcgac gaggtggtcg tgccccttga accagaggac gccgcccgag
     4021 aaggcgcgga cgttgaacgt gctgcggctg gcgtcgagcc ggtagcgggc ggtcgcgccc
     4081 tgcggcgcgg cggctggcgt cgagccggtt gtcttcaccg cgcccgccgc cccgtgcgcg
     4141 agcgcggcac acagcgcgaa tgccgaaatc ttctcccaga gtctcatcgc gaaacctcct
     4201 ttcgaacttg cgtgtttcag cgcggcgcgc tttcgcagag ccgtcgcgcg agcaactcgc
     4261 ccgcgaccgc gtggccgcgc tcgttccagt gaccgttgcc gatgtcgggc gcgaagccgt
     4321 gcaggaagac gttgtgctgc tcggcgtaaa gctgcaactc gggcgcgaga ttgatgacgg
     4381 tgaagccctc gcgctcgccg agcgccttga ggcgcaggtc ggggtagaga agcgtctcga
     4441 cgccgagccg ccgcatgaag tgctcgcgcg cggcggcgtg cgggtgaacc tgtatggcgt
     4501 tgctcatcgt gacggcgacg aacttcgcgc ccctgctttc ggtctcctcg cgcatccgcg
     4561 cgatcaacgc ctcggtcacg cgccacgcct cgtgccagac ctcgcccgca ggctcgcggt
     4621 agatgaggtt gtccgtgccc ggctcttcca agacggtcgt cttcgcttcg ccgcctcgcg
     4681 cgccgtttgt cttcttcgtg tcgttcgcct cggggctggc gttgcggcgc gagcgccacg
     4741 cggcaatcgc ggacttgatc gcgccgtgcg actggtggac ggcctggatg acgcggacgc
     4801 ggtcgcgcac ccagcggccc gcgcggttcg ccgcggactg gcgtaaggtg aaggcgcgcg
     4861 aggtgcggaa cgagtcgtcg agcacgagcc gcccgtcgcg caggacgaag tagggcacct
     4921 cgtccgtccg cttc
//
LOCUS       DBNGOH010000161         8487 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2589397, whole genome shotgun sequence.
ACCESSION   DBNGOH010000161 DBNGOH010000000
VERSION     DBNGOH010000161.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8487)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8487)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8487
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2589397"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            90..1328
                     /locus_tag="AC159P_02940"
     CDS             90..1328
                     /locus_tag="AC159P_02940"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3694440.1"
                     /translation="MKTIYTFYADAAKIALSSIAAHKLRAFLTLIGIIIGVASVVVVG
                     AAISGLNTYVIEKVSKMLGANHFMIARMVSSGELSEEELERRVRRNPKLQFADMEWLQ
                     EHCKTCSHVGAQTGRGTNFDQDGVRFLGGIVFGVTANMAEIEDKTMTEGRFFREEEVN
                     NGQLVVVLGADIKDKFFPDKDPLGQTLKVGGLPMRIIGVEERRGPIFGDLVDKHVYIP
                     ITTYARLYGRPQDMQIHGKSATQLQLTIEDARVALRNYHKLIGSEEDDFGLVNTEELS
                     GQIDEFTGAIALVVTPVTLLSLVVGGIVVMNIMLVSVTERTFEIGLRKALGARRKQIL
                     LQFLIESALLCAFGGVLGLLLATGVSWLITTLTPITMTITIVYVILSVGVSSVIGLIA
                     GIYPAFKASRLDPIVALTRN"
     gene            1445..2716
                     /locus_tag="AC159P_02945"
     CDS             1445..2716
                     /locus_tag="AC159P_02945"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3694441.1"
                     /translation="MNLPGFITKEILAMALDSVRSHKFRSVLTVLGIVIGVLTVITVA
                     ALLTGLRGSLVKIVEEYGVNNIYAFHLTTGIQVGERDQGERARKVLEAEDGEAIKEQA
                     SAVEDVALVSPNIGYGGGPFDDTIRHNDKTYRRGNTQGVTANYAAITNIRLKEGRFIS
                     EHDDVNRRNVIVLGVNAAQAVFPDKPDGVVGTQVRMGGYIWEVIGLLEKRKSTFMGEN
                     EEDNAVYVPFRTGLQVAPARRFLRLVIQAKTGQREQAFDQTEEILRNRRSVKFSSPND
                     FDLKTADAFVGQFDSIFGMVGLIAIAISGVGLLVGGIGVMNIMLVSVTERTAEIGVRK
                     AIGARRRDIVQQFLYEAMTLTLLGGVIGVLLAFAVSQIIMFFVPALPASIPSWAVITG
                     LVVSVAVGLIFGVWPAMKASRLDPIECLRYE"
     gene            complement(2730..3260)
                     /locus_tag="AC159P_02950"
     CDS             complement(2730..3260)
                     /locus_tag="AC159P_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009512210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop nucleotide/nucleoside kinase family
                     protein"
                     /protein_id="HIR3694442.1"
                     /translation="MNLVFLYGPPGAGKLTVGRELEALTGYRLFHNHLTVDLVSAVFD
                     FGTEPFISLREHIWLSVFREAARRGVSLVFTFAPERTVRERFIPEAIEAVESAGGRVL
                     FVELTCGNAELERRIESPSRKASGKLQSAELYRELKEAGVFRHPRMPEAALSLDTTRR
                     PPRETARVIAEHFGLA"
     gene            complement(3368..5359)
                     /locus_tag="AC159P_02955"
     CDS             complement(3368..5359)
                     /locus_tag="AC159P_02955"
                     /inference="COORDINATES: protein motif:HMM:NF017745.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HIR3694443.1"
                     /translation="MKKLLLLLASLAAAVSCAAPPAENANNAATAAGSGDAAFKTLHD
                     RYVVEFLRRNPSVNTYLGGAGLDPSLREVDGRLRDHSAAALEAEDRWLEETQKTFEAA
                     PPQTLSPARRIDREVALAQIRFLLRQHQTRRYQERSVDTYVTEPFRSVDLQLQGMTAT
                     GENSYGTPEEWSLVTKRVEDIPRFLKVAQEQLQAGMKSGNTPDHRMLRRDGIISSEAN
                     AKYFAETLPQIAGERLAAGPQRDSLKTKLDAAVKQAADAYRAFAQFTTDNFFSPGSNG
                     LAPKQPFVADHYAMGAEEYDWAVKNNLRLDKTAAQLYEESWPVVEATRKEMTDLARRV
                     GESRKMQLPADGAEAVRAVFDELSKDYPKSDEEMVKWYRDTCFRVVEYGRKTGVFDVP
                     ADYKLDVTITPPPLRSSVDGAAYYPAPPFKQSGVGRFYVSPTDNDPAALQANNRAALA
                     DLAVHEGFPGHDWHYKVLTQYRGEIAPVRWLTPGAIEDSSSMWEDSMASEGWALYSEA
                     LMAEAQQGAPEGFYTPEERLYQLQGKLLRDLRVRLDTGLHTGRMTYDEATDLYSEIVD
                     FLPGKCADAAKTDAKRASCQSAERAIFRYSKWPTQAITYRLGKEQIYALREEAARASG
                     AAFSPKAFHVLFMKQGTVPAGYFREELLRELQKGVGSRQ"
     gene            5507..5794
                     /locus_tag="AC159P_02960"
     CDS             5507..5794
                     /locus_tag="AC159P_02960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694444.1"
                     /translation="MATETGVAEKAAASELGEHRWGIMSERGCEATRLDYDGAARLVR
                     RLKGEGVRGLCVVTETAASHLAPAKTHDAKATDAKTHSGNGSKRPRREAKK"
     gene            complement(5946..7001)
                     /locus_tag="AC159P_02965"
     CDS             complement(5946..7001)
                     /locus_tag="AC159P_02965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694445.1"
                     /translation="MSKKRLLIAALFALSAAAFAVDAGAQERQPRRVRVETEDVIGTP
                     GGNVVVTRGRTSQGPVAGSEVVIDGQGDNFVFISSEMSIDGKVVKGAPYSAQAVTESV
                     QTLGDGNRIVRKNTANVYRDSEGRTRRDQTLGTIGPFAAQGDPPQTVFINDPVAGITY
                     ILDPRSRSARKLPRFEYKVEFGAKDKEQAELKMKEHSELKLRTPAHNGELETHGFFFR
                     TPGPGGPGPEVHFFGDSSKANANTEKLAAQMVEGVMAEGTRTTVTIPAGEIGNEQPIQ
                     IVSERWYSPELQTVVMTRHSDPRFGENTYKLTNITRTEPAATLFQVPGDYTVRVGPLM
                     RKMRGPGLPRKTPEVQQ"
     gene            complement(7124..8062)
                     /locus_tag="AC159P_02970"
     CDS             complement(7124..8062)
                     /locus_tag="AC159P_02970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694446.1"
                     /translation="MNCHEFESTLDDIARGSLRDAGAGEAHAAACQPCAARLADARSL
                     YEGLRALSTEAAGACAPPRVEANLLTAFRAQTAQTAGAAHVAAGAGQAPVAFVPPVAA
                     PIVVPFVSKKWSWPKTFGAAGLAAAAAVVFVLLVPSIFSGPRQPPPPTLQADTRQPPP
                     KTNVEQPSEGRIIVPPDVLDVHEASVKAPDVALDREPATTATVRPPRAPRGGATMTSV
                     KYGGGGGGARNVGGTTDAAAYGGERAEITTDFIPLAHGGGFAAGDGAHVVRVELPHTA
                     LERFGLPFNAERSGGRVKADVLLGQDGVARAIRFVR"
     gene            complement(8059..>8487)
                     /locus_tag="AC159P_02975"
     CDS             complement(8059..>8487)
                     /locus_tag="AC159P_02975"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR3694447.1"
                     /translation="SYLYGIARNHVLRVFEKERPTVALVEEETGEPPAHENLIARGDP
                     LGDLTRADEVGRLRQAVLALPAHYREVVVLCELHEMSYAEAAEALECAVGTVRSRLHR
                     ARSMLAEKMRGGARSGAATGETTERDAAGGAALRTARCFA"
BASE COUNT         1324 a         2803 c         2883 g         1477 t
ORIGIN      
        1 aaatctccgt aaacattgcg cgcggtcgcg gcgtattagg gtgtgcccgg cagttcggtg
       61 cttcccgcaa gaccagcgca aaagctgtta tgaaaacgat ttacaccttc tacgcggacg
      121 ccgcgaagat cgcgctctcg tcgatcgccg cgcacaagct gcgcgcgttc ctcacgctca
      181 tcggcatcat catcggggtg gcctcggtgg tggtggtcgg cgcggccatc tccgggctga
      241 acacctacgt catcgagaag gtgtcgaaga tgctcggcgc gaaccacttc atgatcgcgc
      301 gcatggtcag ctcgggcgag ctgtcggagg aggagctgga gcggcgcgtc cgccgcaacc
      361 cgaagctcca gttcgccgac atggagtggc tgcaagagca ctgcaagacg tgcagccacg
      421 tcggcgcgca gaccgggagg ggcacgaact tcgaccagga cggcgtgcgt ttcttgggcg
      481 gcatcgtctt cggcgtgacg gcgaacatgg ccgagatcga agacaagacg atgaccgagg
      541 ggcgcttctt ccgagaggag gaggtgaaca acggccagct cgtcgtcgtc ctcggcgcgg
      601 acatcaagga caagttcttc cccgacaaag accccctcgg ccagacgctc aaggtgggcg
      661 gcctgccgat gcgcatcatc ggcgtcgagg agcggcgcgg ccccatcttc ggcgacctcg
      721 tggacaagca cgtctacatc ccgatcacca cctatgcgcg cctctacggt cgcccgcagg
      781 acatgcagat tcacggcaag agcgcgacgc agctccagct caccatcgag gacgcgcgcg
      841 tcgccctgcg caactaccac aagctcatcg gcagcgagga ggacgacttc ggcctcgtca
      901 acaccgagga gctgagcggg cagatcgacg agttcaccgg cgccatcgcg ctcgtcgtca
      961 cgccggtgac gctgctctcg ctcgtcgtcg gcggcatcgt cgtgatgaac atcatgctcg
     1021 tctccgtcac cgagcgcacc ttcgagatcg ggctgcgcaa ggctttgggc gcgcgccgca
     1081 aacagattct cttgcagttc ctgatcgagt cggcgctgct gtgcgcgttc ggcggcgtgc
     1141 tcggcctgct gctggcgacc ggcgtgagct ggctcatcac gacgctgacg ccgatcacca
     1201 tgaccatcac catcgtctac gtgattctgt cggtcggcgt ctccagcgtc atcggcctca
     1261 tcgccggcat ctacccggcc ttcaaggcgt cgcgcctcga ccccatcgtc gcgctcacgc
     1321 gcaactgaaa gagccgtgaa ccgtgaaccg taaagcgtga caagataaaa accagtgctt
     1381 gctcgtcacg ctttacggtt catcgtcccg gctttcactt gtcacggttc acggttgttg
     1441 acttatgaat ctgcccggct ttatcaccaa agagattctg gcgatggcgc tcgacagcgt
     1501 ccggtcgcac aagttccgga gcgtgctgac ggtgctcggc atcgtcatcg gcgtgctgac
     1561 ggtgattacg gtggcggcgc tgctgacggg gctgcgcggg agcctcgtga agatcgtcga
     1621 ggagtacggc gtcaacaaca tctacgcctt ccacctgacg acgggcattc aggtcggcga
     1681 gcgcgatcag ggcgagcgcg cgcgcaaagt tctggaggcg gaggacggcg aggccatcaa
     1741 ggagcaggcg agcgcggtcg aggacgtggc gcttgtctcg ccgaacatcg gctacggcgg
     1801 cgggccgttc gacgacacca ttcgtcataa cgataaaacg taccggcgcg gcaacacgca
     1861 gggcgtgacg gcgaactacg ccgccatcac caacattcgt ttgaaggaag gtcggttcat
     1921 cagcgagcac gacgacgtga accgccgcaa cgtgatcgtc ctcggcgtca acgcggcgca
     1981 ggccgtcttt ccggacaagc cggacggcgt cgtcggcacg caggtgcgga tgggcggcta
     2041 catctgggag gtgatcggcc tgctggagaa gcgcaagagc accttcatgg gcgagaacga
     2101 agaggacaac gccgtctacg tccccttccg cacgggcctc caggtcgcgc ccgcgcgccg
     2161 cttcctgcgc ctcgtcatcc aggccaagac cgggcagcgc gagcaggcgt tcgaccagac
     2221 ggaagagatt ctgcgcaacc ggcgcagcgt gaaattcagc tccccgaacg acttcgacct
     2281 caagacggcg gacgccttcg tcggccagtt cgacagcatc ttcgggatgg tcggcctgat
     2341 cgccatcgcc atctccggcg tcggcctgct ggtcggcggc atcggcgtga tgaacatcat
     2401 gctcgtctcg gtgaccgagc gcacggcgga gatcggcgtg cgcaaggcca tcggcgcgcg
     2461 ccgccgcgac atcgtccagc agttcctcta cgaggcgatg acgctgacgc tgctgggcgg
     2521 cgtcatcggc gtgctgctcg cgttcgcggt gagccagatc atcatgttct tcgtgcccgc
     2581 tctgcccgcc tcgatccctt cgtgggcggt catcaccggc ctcgtcgtct cggtcgcggt
     2641 cggtctgatc ttcggcgtct ggcccgcgat gaaggcgtcg cggctcgacc cgatagagtg
     2701 cctgcgttac gagtgagggg cgcgggccgt caggccagtc cgaagtgttc cgcgatcact
     2761 cgcgccgtct ctcgcggcgg tcgccgggtg gtgtcgagcg acagcgccgc ctccggcatc
     2821 cgcggatgcc ggaagactcc cgcctctttc agttcacgat acagctcggc tgactggagc
     2881 ttgcccgacg ctttgcgcga aggactctcg atgcgccgct caagctccgc gttcccgcag
     2941 gtcaactcca cgaagagcac ccgcccgccc gccgactcga ccgcctctat agcttcgggg
     3001 atgaaccgct cgcggacggt gcgctcgggc gcgaaggtga agacgagcga cacgccgcgc
     3061 cgcgccgcct cgcggaagac cgagagccag atgtgttcgc gcaagctgat gaacggctcg
     3121 gtgccgaagt cgaagaccgc gctgacgaga tcgaccgtca ggtggttgtg gaacagccgg
     3181 tagccggtca gcgcttcgag ttcgcgcccg accgtcagct tgccggcgcc cggcgggccg
     3241 tagaggaaga cgagattcat acttaatcag taggcagtag gcagcagaca gacaagaagt
     3301 tctgaattca aaagttgaga tttcaggttt caaagctcga cttcgaatcg cttttactgc
     3361 ctaccgccta ctgcctactg cctactcctt tctgcaactc gcgcaaaagc tcctcgcgga
     3421 agtagcccgc ggggacggtg ccctgcttca tgaagaggac gtggaatgcc ttgggcgaga
     3481 aggcggcgcc cgacgcgcgc gcggcctctt cgcgcagggc gtagatttgc tccttgccga
     3541 ggcggtaggt gatggcctgc gtcggccact tcgagtagcg gaagatggcg cgctcggctg
     3601 actggcagct cgcgcgcttg gcgtcggtct tcgcggcgtc ggcgcacttg ccggggagga
     3661 agtccacgat ctcggagtag aggtcggtcg cctcgtcgta ggtcatgcgg cccgtgtgca
     3721 gccccgtgtc gaggcgcacg cgcaggtcgc gcaggagctt gccctgaagt tggtagagcc
     3781 tttcttcggg cgtgtagaag ccttcgggcg cgccctgctg ggcctcggcc atcagcgcct
     3841 cggagtagag cgcccagccc tccgacgcca tcgagtcctc ccacatcgag cttgaatcct
     3901 caattgctcc gggcgtgagc cagcgcacgg gggcgatctc gccgcggtac tgggtcaaca
     3961 ccttgtagtg ccagtcgtgg ccggggaagc cttcgtgcac cgcgaggtcc gcgagcgcgg
     4021 cgcggttgtt cgcctgaagg gccgccgggt cgttgtcggt gggcgagacg tagaagcgcc
     4081 cgacgccgct ctgtttgaac ggcggcgcgg ggtagtaggc cgcgccgtca accgacgagc
     4141 gcaggggcgg cggcgtgatc gtcacgtcga gcttgtagtc ggcgggcacg tcgaagacgc
     4201 cggtcttgcg cccgtactcg acgacgcgga agcaggtgtc acgataccac ttgaccatct
     4261 cttcgtcgga cttcgggtag tccttcgaca gctcgtcgaa gacggcgcgc acggcctcgg
     4321 cgccgtccgc ggggagctgc atctttcgac tctcgccgac gcggcgcgcg aggtcagtca
     4381 tctctttgcg cgtcgcctcg accaccggcc aggactcttc gtagagctgc gcggcggtct
     4441 tgtcgagacg gagattgttt ttaacggccc agtcgtactc ctccgcgccc atcgcgtagt
     4501 ggtcggcgac gaacggctgc ttcggcgcga gcccattgct gccgggcgaa aaaaaattgt
     4561 cggtcgtgaa ctgcgcgaag gcgcggtaag cgtctgccgc ctgcttcacc gccgcgtcga
     4621 gcttcgtctt taacgagtcg cgctgcggcc cggcggcgag ccgctcgccc gcgatctgcg
     4681 gcagtgtttc ggcgaaatat tttgcgttcg cttccgaaga gatgatgccg tcacgccgga
     4741 gcatccggtg gtcgggcgtg ttgccggact tcatgcccgc ttgcaactgc tcctgcgcga
     4801 ccttgaggaa gcgcgggatg tcctcgacgc gtttcgtgac gagcgaccac tcttcgggcg
     4861 tgccgtaact gttctcgccg gtcgcggtca tgccttgaag ctgaaggtct acggagcgga
     4921 aaggctccgt gacgtaagta tcgacggagc gctcctgata gcggcgcgtc tggtgctggc
     4981 gcagcagaaa cctgatttgc gcgagcgcca cttcgcggtc gatgcggcgc gcgggcgaga
     5041 gcgtctgcgg tggggcggcc tcgaaggtct tctgcgtctc ttcgagccag cggtcttccg
     5101 cttcgagcgc cgccgccgaa tggtcgcgca ggcgcccgtc aacttctctg agcgaggggt
     5161 cgaggccggc gccgccgagg taggtgttga ccgaagggtt gcggcggagg aattcgacga
     5221 cgtagcggtc gtgcagcgtc ttgaacgcgg cgtcgccgct gcccgccgcc gtcgccgcgt
     5281 tgttcgcgtt ctccgcgggc ggcgccgcgc aagagacggc ggcggccagt gatgccagaa
     5341 gcaggaggag ttttttcatc gccctcgcct ttccgaagtt gagtcgtgtg ttcgaagtcc
     5401 gccgctgcgg cgctcgtcga ggacgagagc ttaacgccgc ccgcgcgcgc gccgcaagcg
     5461 ggcgcccgtt tggcgttcgc cgtgcgcatc cgctaaaatt gcgccgatgg ctacggagac
     5521 gggagtggcg gaaaaggccg cggcgagcga gctcggcgag caccgctggg ggatcatgag
     5581 cgagcgcggc tgcgaggcga cgcgccttga ttatgacggc gcggcgcggc tcgtgcgccg
     5641 gctcaagggc gagggcgtgc gcggcctctg cgtcgtcacg gagacggcgg cgagccacct
     5701 cgcgcccgcc aaaacgcacg acgcgaaagc gacggacgcc aaaacgcatt cgggcaacgg
     5761 ctcgaagcgc ccgcgccgcg aggcgaagaa gtgaggtcgc ccggttaagc gcgcgtgggt
     5821 tcatcaagcg gcgcgcgcaa gtcttcaaga aagaagaagc ccacggcata gggtgccgtg
     5881 ggcttcttct ttcgcggcgc tcggcccgcg cggtctcgtc gggcgagcga actcgggagg
     5941 tctcgttact gctgaacctc gggcgtcttc ctcggcaggc cggggccgcg catcttgcgc
     6001 atgagcgggc cgactctgac cgtgtagtcg ccgggcacct ggaagagtgt ggcggcgggt
     6061 tcggtgcgcg tgatgttggt cagcttgtag gtgttctcgc cgaagcgcgg gtcgctgtgg
     6121 cgcgtcatca ccacggtctg gagttcgggc gagtaccagc gctcgctgac gatctggatg
     6181 ggctgctcgt tgccgatctc gccggcgggg atcgtgaccg tggtgcgcgt gccctcggcc
     6241 atcacgccct cgaccatctg cgccgcgagc ttctcggtgt tggcgttcgc cttcgacgag
     6301 tcgccgaaga agtgaacctc aggccccggc ccgcccggcc ccggcgtccg gaagaagaag
     6361 ccgtgtgtct caagctcccc gttgtgcgcg ggcgtccgca gcttcagctc gctgtgctcc
     6421 ttcatcttca gttcggcctg ctccttgtcc ttggcgccga actcgacctt gtactcgaag
     6481 cgcggcagct tgcgcgccga gcgcgaacgc ggatcgagga tgtaggtgat gccggcgacg
     6541 gggtcgttga tgaagacggt ctgcggcggg tcgccctgcg cggcgaaggg gccgatggtg
     6601 ccgagcgtct ggtcgcggcg cgtgcgcccc tcgctgtcgc ggtagacgtt ggccgtgttc
     6661 ttgcgcacga tgcggttgcc gtcgcccagg gtctgcaccg actcggtgac ggcctgcgcc
     6721 gagtagggcg cgcccttcac gaccttgccg tcgatgctca tctccgacga gatgaagacg
     6781 aagttgtcgc cctgcccgtc gatgacgact tcggaacccg ccaccgggcc ttgcgacgtg
     6841 cgcccgcgcg tgacgacgac gttgccgccg ggcgtcccga taacgtcctc ggtctcgacg
     6901 cgcacgcgcc gcggctgccg ctcctgcgcg cccgcgtcca cggcgaaggc cgccgccgag
     6961 agggcgaaca gcgcggcgat caacagtctc ttcttactca tgactcttat ctctccttct
     7021 aaaatttatg cccgaccttg cttcgaatca ttccgcgaac gggacgcgcc cctcgaacga
     7081 agcgcgtgat ttcgatttcg atcgatttcg atgaggcgac ggctcagcgc acgaagcgga
     7141 tggcgcgggc cacgccgtcc tgaccgagga gcacgtcggc cttgacgcga ccgccggagc
     7201 gctcggcgtt gaacggcagc ccgaagcgtt cgagcgccgt gtgcggcagc tcgacgcgga
     7261 cgacgtgggc gccgtcgccg gctgcgaacc cgccgccgtg cgcgagcggg atgaaatcgg
     7321 tggtgatctc cgctcgctcg ccgccgtacg ccgccgcgtc cgtggtgccg ccgacgttcc
     7381 tcgcaccgcc gcctccgccg ccgtacttca cgctcgtcat cgtcgccccg ccgcgcggcg
     7441 cgcgcggcgg gcggacggtg gccgtcgtcg ccggctctct atcgagcgcc acatcaggcg
     7501 ccttcacgct cgcctcgtgt acgtcgagca cgtcgggcgg gacgatgatt cttccttcac
     7561 tcggttgttc gacgttcgtc ttcggcggcg gctgccgcgt gtctgcctgc aacgtcggcg
     7621 gcggcggctg ccgcggcccg gagaagatcg aagggacgag caagacgaag acgaccgccg
     7681 ccgccgcggc cagacccgct gcgccgaacg tcttcggcca cgaccacttc ttcgagacga
     7741 aaggaacgac gatgggcgcg gcgaccggcg ggacgaaggc gaccggtgcc tgtccggcac
     7801 ccgcggcgac gtgagcggcg cccgcggtct gcgctgtctg cgcgcggaag gccgtcagca
     7861 ggttcgcctc gacgcgcgga ggggcgcacg cgcccgccgc ctccgtagag agtgcgcgca
     7921 aaccctcgta cagagagcgc gcgtccgcga gtcgcgcggc gcagggctgg cacgcggcgg
     7981 cgtgcgcctc tccggcgccc gcgtcccgca gagacccgcg cgcgatgtcg tccagcgtgc
     8041 tttcgaattc gtgacagttc atgcgaagca cctcgccgtc ctcagcgccg cgccgcccgc
     8101 ggcgtccctc tccgtcgtct ctcccgtcgc ggcgccgctg cgcgccccgc cgcgcatctt
     8161 ctcggcgagc atcgagcgcg ccctgtgcag gcgcgaacgc accgtgccga ccgcgcattc
     8221 gagtgcctct gcggcctcgg cgtaactcat ctcgtgcagc tcgcacagca cgaccacctc
     8281 gcggtagtgc gcggggagcg cgagcacggc ctgcctgagc ctcccgacct cgtcggcgcg
     8341 cgtcaaatcg cccagcgggt cgccgcgcgc gatcaggttt tcgtgcgcgg gcggctcgcc
     8401 cgtctcttct tcgacgagcg cgacggtcgg tcgctctttt tcgaagacgc gcagcacgtg
     8461 gttgcgcgcg atgccgtaga gatacga
//
LOCUS       DBNGOH010000162         4776 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3689226, whole genome shotgun sequence.
ACCESSION   DBNGOH010000162 DBNGOH010000000
VERSION     DBNGOH010000162.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4776)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4776)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4776
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3689226"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..484)
                     /locus_tag="AC159P_02980"
     CDS             complement(<1..484)
                     /locus_tag="AC159P_02980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694448.1"
                     /translation="MTPQLFANFEVNRAPRWPIMSRLLMASVVAHGLFMVTVAYVPSV
                     RGLLGVAGTLSGFEFVSEDYDRSLVGQRATVINVAPGEKLYYPADYFTVNSPFADPSM
                     AGDAQLVAQVQPTPTPPPMPVYRPRRGAGPRVVATATPEATPTPEEVASATPTPSPET
                     E"
     gene            664..3525
                     /locus_tag="AC159P_02985"
     CDS             664..3525
                     /locus_tag="AC159P_02985"
                     /inference="COORDINATES: protein motif:HMM:NF019758.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIR3694449.1"
                     /translation="MTRSQKGRRRGPKRGARSNTGGGGDDGERRGGDAPRSGTGERQA
                     RSDAAHRRDGGGDGPRRPADSAAEDASRVAGRAARSRPLRQLLEGIGTPEAKPFTPDP
                     FQLEALAALESEDVLVTAPTGSGKTWIAREEIRRLLAQGKRAWYTSPLKALTNSKYHE
                     FAAEFGVEKVGILTGDRKENGDAPLVVGTTEVYRNQLFDALRGGQQVNADLVILDEAH
                     YLSDPERGHVWEEAIILTPPRVRLLLLSATVGRAEDFADWIGEVRGTRVRVVSRPGAR
                     PVPLRAAFLYPDGGLSPLFDEHHRFNAEIARFIQTERSERQQRHRFGQRGGGGHRRPG
                     MPEMPPSILLAALGSYDLLPAIVFLPTRRRCDEAAAEAAFTPRRGSNDAARREARREV
                     LNELVEQYPEMRKHRHYDLVARGGVASHHAGHMPAWKLAVERLMSRGLLDAIFATATV
                     AAGVDFPARTVVLENIDVRTGHGWRILTASELQQMTGRAGRRGRDRVGFIVAAPGSHQ
                     DPQKLAALLNAPPDPLESQFRATYTTLLNLLDAYGTFAQVRDIAERSFARRDAGEEVA
                     RLEREREEAEAGMKEKLSQAGCDLPPEVARGLERLASARTRLLEGAPHTRADMWMRWL
                     DEQVTAGRVVGVGRASRRLVLVTERRGDGFAGVREDGRRASLALERVGRVYADTFPLD
                     ERRRDEAFEAVRAGRAVLLQEPRLRDARAPSDEAVTLINDLIDGLAAGDERVRCREAL
                     WSVVKDAETVERMERRLEALRHEVWQPFERRARVLHHFGYLDFFAERVTERGRWLADL
                     RLDRPLLVGETIGRGLFSKLDARRAAGLMAALAADAERDYGSLELDDALVNALTSFDR
                     IAYDVSNVEWQQELEPAPEINFSAAAAAARWAGGATWPTLVRETRAEEGDLFRMLSRT
                     GESLLQITGLRDSHPDAARIAEAAAEAILREPVRSESIL"
     gene            3573..4733
                     /locus_tag="AC159P_02990"
     CDS             3573..4733
                     /locus_tag="AC159P_02990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694450.1"
                     /translation="MFRQLPHTFFGFALLLSSSAAPAPSSAQVNGGTAKAAAESRARD
                     REIAARFAPVFFQALGDRRRGNFITNFDFDGDWRGDNNWSHAEVQAHRLRAFVYYAVS
                     ETPTHFLVHYAVFHPRDYKGGSLRGALLSQAIKEGVRRGGKFDPTGLSESAVLAHEND
                     MEGCLVVAAKGGGGDLAGAAVVFVETLAHDRFMKYVPAGSPRAGFQTVRVDGQRPALY
                     VEAKGHGVYAYDERDPKHAPGSGVLRCEYVARADDPEGHGATAKNLVGYDLVSIHDTL
                     WPRARRSPNPTFGAKYDYGTRSVSVAQAAGRPLTRRFKLGELGAAFAGGVGAANAARP
                     PWAWFDREERAQPLGEWFFDPASVVKRHFGLGDDFALAYLHHPFLGVRRKGK"
BASE COUNT          735 a         1733 c         1660 g          648 t
ORIGIN      
        1 cttcggtctc gggcgaaggg gtgggcgtgg ccgaggccac ctcttcgggc gtcggcgtgg
       61 cttcgggcgt ggcggtcgcg acgacgcgcg gcccggcgcc gcggcgcggc ctgtagacgg
      121 gcatcggcgg cggcgtgggc gtgggctgca cctgcgcgac gagctgcgcg tcgcccgcca
      181 tcgacgggtc ggcgaagggc gagttgacgg tgaagtagtc cgccgggtag tagagcttct
      241 cgccgggcgc gacgttgatg acggtggcgc gctggccgac gagcgagcgg tcgtagtcct
      301 cgctgacgaa ctcgaagccg gagagcgtgc cggcgacgcc gagcaggccc cgcaccgaag
      361 gcacgtacgc gaccgtcacc atgaacagcc cgtgcgccac gacggacgcc atcagcagcc
      421 gcgacatgat cggccagcgc ggcgcgcggt tgacttcgaa gtttgcgaaa agctgtggtg
      481 tcatgccctt gtcttgttcg aacccgccgc ccccgcccga gccactacgg gccgaatgtc
      541 ttagacgccc gtcggcggcc agacgttgcg aagcgacaca caaaagttat cactacgagg
      601 gcggtttcaa caaagttcgt gaggtaaaat atctgtaaag tgtaaggtaa actgtctgtc
      661 acaatgacac gttcacagaa aggcaggcgg cgcggcccca aacgcggggc gcgctcgaac
      721 acgggcggcg gcggcgacga cggcgagcga cgcgggggcg acgcgcctcg cagcggcacc
      781 ggcgagcgtc aagcgagaag cgacgccgcg cacaggcgcg acggcggcgg cgacgggccg
      841 cgccgcccgg cggattcggc ggcggaagac gcctcgcgcg tggcgggccg cgcggcgcgc
      901 tcgcgcccct tgcgccaact gctcgaaggc atcggcacgc ccgaggcgaa gcccttcaca
      961 cccgacccct tccagctcga agcgctcgcc gcgctcgaaa gcgaggacgt gctcgtcacc
     1021 gcgccgacgg ggagcggcaa gacgtggatc gcgcgcgaag agattcgccg cctcctcgca
     1081 caggggaagc gcgcgtggta caccagcccc ttgaaggcgc tcaccaactc caagtaccac
     1141 gagttcgccg ccgagttcgg cgtcgaaaag gtcggcatcc tcacgggcga caggaaagag
     1201 aacggcgacg cgccgctcgt cgtcggcacg accgaggtct accgcaacca gctcttcgac
     1261 gccttgcgcg gcggccagca ggtcaacgca gacctcgtga ttttggatga agcgcactac
     1321 ctctccgacc ccgagcgcgg ccacgtctgg gaggaggcga tcatcttgac gccgccgcgc
     1381 gtgcgcctgc tgctgctctc ggccacggtc ggacgcgccg aagacttcgc cgactggatc
     1441 ggcgaagtgc gcggcacccg agtccgcgtc gtctcgcgcc ccggcgcgcg gcccgttccc
     1501 ttgcgcgccg ccttccttta tcccgacggc ggactctcgc cgctcttcga cgaacaccat
     1561 cgcttcaacg cggagatcgc gcgcttcatc cagaccgaaa ggagcgagcg gcagcagcgc
     1621 catcgcttcg gccaacgcgg cggcggcggc catcgccgac ccggcatgcc cgagatgccg
     1681 ccgtcgatac tgctcgccgc gctcggctct tacgacctgc tgcccgcgat agtcttcctg
     1741 ccgacgcgcc gtcgctgcga cgaggccgcg gcggaagcgg ccttcacacc gcggcgcggc
     1801 agcaacgacg ccgcgcgccg cgaagcgcgc cgtgaagtcc tgaacgagct ggtcgagcag
     1861 tatccggaga tgcgcaagca tcgtcattac gatctggtcg cgcgcggcgg ggtcgcctcg
     1921 caccacgcgg gacacatgcc cgcgtggaag ctcgccgtcg agcggctgat gagccgcgga
     1981 ttgctcgacg ccatcttcgc cacggcgacg gtcgccgcgg gcgtggactt cccggcgcgc
     2041 acggtcgtcc tcgaaaacat agacgtgcgg acgggccacg gctggcgcat cctgacggcc
     2101 tcggagctgc aacagatgac gggccgcgcg gggcggcgcg ggcgcgaccg cgtcggcttc
     2161 atcgtcgccg cgcccggctc gcaccaagac ccgcagaagc tggccgcgct gctcaacgct
     2221 ccgcccgacc cgctggagag ccagttccgc gccacgtaca cgacgctgct taatttgctc
     2281 gacgcctacg gcaccttcgc gcaggtgcgc gacatcgccg agcgcagctt cgcgcgccgc
     2341 gacgccggcg aagaggtcgc gcggctcgaa cgcgagcgcg aagaggccga ggcgggcatg
     2401 aaagagaagc tctcgcaggc ggggtgcgat ttgccgccgg aggtggcgcg cggccttgag
     2461 cgtctggcga gcgcgcgcac gcgtctgctc gaaggcgcgc cgcacacgcg cgcagacatg
     2521 tggatgcgct ggctcgatga gcaggtgacg gcgggccgcg tggtcggggt gggccgcgcg
     2581 agccgccgtc tggtgctcgt taccgagcgg cgcggcgacg gcttcgccgg agtacgcgag
     2641 gacgggcgcc gcgcctcgct cgcgctcgaa cgcgtcggca gggtctacgc cgacaccttc
     2701 ccgctcgacg agcgcagacg cgacgaagcc ttcgaggccg tgcgcgcggg tcgcgccgtc
     2761 cttcttcaag aaccgcgtct gcgcgacgcg cgcgcgccgt cggacgaggc cgtgacgctc
     2821 atcaacgatt tgatcgacgg cctcgcggcg ggcgacgagc gcgtgcgctg ccgcgaggcg
     2881 ctgtggtctg tcgtcaaaga cgcggagacg gtcgagcgca tggagcggcg cctcgaagcg
     2941 ctgcgccacg aagtgtggca gcccttcgag cggcgcgcgc gcgtgctcca tcacttcggc
     3001 tatctcgact tcttcgccga gcgcgtgacc gagcgcgggc gctggctcgc ggacctccgg
     3061 ctcgaccgac cgctcctcgt cggcgagacc atcgggcgcg gcctcttctc gaagctcgac
     3121 gcgcgccgcg ccgcggggct gatggccgcg ctcgccgcgg acgccgagcg cgactacggc
     3181 tcgctcgaac tcgacgacgc gctcgtcaac gcgctcacga gcttcgaccg catcgcctac
     3241 gacgtgtcga acgtcgagtg gcagcaggag ctggaacccg cgcccgagat caacttctcg
     3301 gcggcagccg ccgccgcgcg ctgggccggc ggcgcgacgt ggccgacgct cgtccgcgag
     3361 acgcgcgccg aagagggcga cctcttccgc atgctctcgc gcacgggcga atcgctcctc
     3421 cagatcaccg gcctgcggga ctcgcacccc gacgccgcgc gcatcgccga ggcggcagcc
     3481 gaagcgatcc tgcgcgagcc tgtgcgctcc gagtcgatcc tttgagcctg tccgccgcgc
     3541 acgactaaac cgttccgcga aaggcatcga acatgtttcg tcaactcccc cacacgttct
     3601 tcggcttcgc cctgctcctg tcgtcgtccg ccgcgcccgc accttcttcc gcgcaggtca
     3661 atggcggcac cgcgaaggcc gcggctgaga gccgcgcgcg cgaccgcgag atcgccgcgc
     3721 gcttcgcccc cgtcttcttt caggcgctcg gcgaccgcag gcgcggcaac ttcatcacca
     3781 acttcgactt cgacggcgac tggcgcggcg acaacaactg gtcgcacgcc gaagtccaag
     3841 cccaccgcct gcgcgccttc gtctactacg ccgtgagcga gacgccgacg cacttcctcg
     3901 tccactacgc cgtcttccac ccgcgcgact acaagggcgg cagcctgcgc ggcgcgctcc
     3961 tcagccaggc catcaaggag ggcgtcaggc gcggcggcaa gttcgacccg accggcctct
     4021 ctgagagcgc cgtgctcgcg cacgagaacg acatggaggg ctgtctcgtc gtcgcggcga
     4081 agggcggcgg cggcgacctc gcgggcgccg cggtcgtctt cgtcgagacg ctcgcgcacg
     4141 accgcttcat gaagtacgtg cccgcaggca gcccgcgcgc cggcttccag acggtgcgcg
     4201 tggacggcca gcgccccgcg ctctacgtcg aggcgaaggg gcacggcgtc tacgcctacg
     4261 acgagcgcga cccgaagcac gcgcccggga gcggcgtgtt gcggtgcgaa tacgtcgcgc
     4321 gcgcagacga ccccgaaggc cacggcgcca cggcgaagaa cctcgtcggc tacgacctcg
     4381 tctccatcca cgacacgctc tggccgcgcg cgcgccgcag tcccaacccg accttcggcg
     4441 cgaagtacga ttacggcacg cggagcgtca gcgtcgcgca ggccgcggga cgccccctca
     4501 cgcgacgctt caagctcggc gagctgggcg cggccttcgc gggcggcgtc ggcgcggcga
     4561 acgccgcccg cccgccgtgg gcctggttcg accgcgaaga gcgcgcccag ccgctcggcg
     4621 agtggttctt cgacccggct tcggtcgtca aacgccactt cggcctcggc gacgacttcg
     4681 cgctcgccta cctgcaccac cccttcctcg gcgtgcggcg caaagggaag tgataagcat
     4741 tttggattgc ggattacgga ctggcgtgcc gctcgg
//
LOCUS       DBNGOH010000163         4054 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2223097, whole genome shotgun sequence.
ACCESSION   DBNGOH010000163 DBNGOH010000000
VERSION     DBNGOH010000163.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4054)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4054)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4054
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2223097"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            449..2068
                     /locus_tag="AC159P_02995"
     CDS             449..2068
                     /locus_tag="AC159P_02995"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HIR3694451.1"
                     /translation="MREPVDAGACAPQPGSVGILEAVLEGTPDAVFVKDLEGRYLLVN
                     SACARFIGRPKDEIVGRRDEELYPPETARRFVEADRQIFATGQTQIFEGEATAEGGAT
                     QSFRVTKGVVRDASGRVVGLFGISHDMTERRRAEEERLQRAREQAARAEAEEASRMKD
                     IFLATLSHELRTPLTAIYGWASMIDDAGVEEATVRNGVKVIRRNAEQQRHIIDDIFDA
                     SGVITGKLRVETRPIELAQVARAAVDVVRPLVEAKGVRLSCDFDAGGAVVLGDPHRLQ
                     QMVWNLLSNAVKFTPPGGEVRVEVGERADGVRLTVSDTGRGIGPEFLPYVFERFRQAD
                     SSTTRPHGGLGLGLSLVKHLVELHGGTVRAESEGDGRGATFTVELPPAPPADSSAPAP
                     HPEPHAAPQEPARRTGESPARALEGLRVLLVDDHEDTLQLLSMFLRRQGAQVRAVDSA
                     ARALEEIAEARPDVLVSDIGMPGEDGYELMRKVRALAPGRGGRTPAVALTAYAGEADR
                     RLAFEAGFDAHVAKPVEPEALLSTLADLAGR"
     gene            complement(2228..3118)
                     /locus_tag="AC159P_03000"
     CDS             complement(2228..3118)
                     /locus_tag="AC159P_03000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694452.1"
                     /translation="MSENSSDGYGRRGFLTRGAAALVGTAFLREARGQKALAAPHQHR
                     GDIATHNMLVVGEKTVFLSHLPMFKEEDAEGNLVATPHRFQVVLEAAFMRGSSAQPQT
                     DYAADRTQHRDTKIYMLNPRETFKLAELVASRPRRTFRGTVFRGHFERDGKVAVLDNV
                     DVTVKNVVHFHEFDPKAARLGKLEYLVFGKGGELFMAHLITSPPDFDQMVAVELPGHK
                     FTDAQLGKGVRVVFNDRENSVASRLKEGEEAAGEVGGGRAPKQKIQVKAAREIFFEEG
                     ELRAPPNFDTTDEEGSAGFP"
     gene            3384..>4054
                     /locus_tag="AC159P_03005"
     CDS             3384..>4054
                     /locus_tag="AC159P_03005"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HIR3694453.1"
                     /translation="MKVIPETTTIAHYRIMESLGAGGMGAVFKAYDEKLHRVVALKLL
                     PPEYTSHEDRRRRFLQEARAASALNHPHILTVYEFGEDGGRPYMAMEYVEGETLRQRI
                     AASPLQLGEGLGIAVQVAEGLAKAHAAGIVHRDLKPENLMIADGGYAKVLDFGLAKLN
                     AETLSANIATDATRTVASPAGTNPGQVMGTVGYMSPEQAQGLPVDHRSDIFSFGCILY
                     ESATGA"
BASE COUNT          656 a         1378 c         1405 g          615 t
ORIGIN      
        1 ccttatggac gttgtggtcg ggcgcgcgaa ggagtggaaa gaaatcgcgg ccagagggga
       61 acgcgtgccg cggcctgcgg cttgcgcggc ctcgcgccgg aaagattcaa tttctcactc
      121 agggcagggc atgaggtcgt catgaccctc ggaaccgaat cgaatgggcc agacaatata
      181 ctggaaaaaa tccaacgtca agaatcccgg gacgctccga acggtttcct cgaacggcct
      241 tccgggaagc gtcgccctcc gtcgccccgc gcgttcctcg tcaggtctgt tcaagaagct
      301 tcccaagcgg ggacgtcggg gatagaattg ccggactccg gcgggctgcc gttgcggccc
      361 gccattcgac gccccgccgc cggcacgacg cccggcgcgg cggcggcaag actgacttac
      421 tcacgtcggc tagtcggtaa aggcatctat gcgcgaacca gtggacgccg gggcgtgtgc
      481 gccgcagccg gggagcgtcg gcatccttga ggccgtgctc gaaggcacgc ccgacgccgt
      541 cttcgtcaaa gacctcgaag ggcgctacct gctggtcaac tccgcctgcg cgcgcttcat
      601 cggcaggccc aaagacgaga tcgtcggcag gcgcgacgaa gagctgtacc cgccggagac
      661 ggcgcgccgc ttcgtcgagg ccgaccggca aatcttcgcc accgggcaga cgcaaatctt
      721 cgagggcgag gccaccgccg agggcggcgc gacgcagtcg ttccgcgtga cgaagggcgt
      781 ggtgcgcgac gcgtccgggc gcgtcgtcgg cctcttcggc atctcgcacg acatgaccga
      841 gcgccgccgc gccgaggaag aacgtctgca acgcgcgcgc gaacaggcgg cgcgcgccga
      901 ggccgaagag gcgagccgga tgaaagacat cttcctcgcc acgctctcgc acgagctgcg
      961 cacgcctctg acggccatct atgggtgggc gagcatgatc gacgacgcgg gcgtggaaga
     1021 ggcgaccgtg aggaacggcg tcaaggtcat caggcgcaac gccgagcagc agcgccacat
     1081 catcgacgac atcttcgacg cctcgggcgt catcaccggc aagctccgcg tcgagacgcg
     1141 cccaatcgag ctggcgcagg tggcgcgggc cgccgtggac gtggtgcgcc cgctcgtcga
     1201 ggcgaagggc gtccgtctga gctgcgactt cgacgcgggc ggggcggtcg tcctcggcga
     1261 cccgcaccgc ttgcagcaga tggtctggaa cctgctctcg aacgcggtga agttcacgcc
     1321 gccgggcggc gaggtgaggg tcgaagtcgg ggagcgggcg gacggcgtgc ggctgacggt
     1381 gagcgacacg gggcgcggca tcggccccga gttcctgccc tacgtcttcg agcgcttccg
     1441 gcaggccgac agcagcacca cgcggccaca cggcggcctc ggcctcggcc tctcgctcgt
     1501 caaacatctg gtcgagctgc acggcggcac cgtccgcgcc gagagcgaag gcgacggtcg
     1561 cggcgccacc ttcaccgtcg agctgccgcc cgcgccgccc gccgactcgt ccgcgcccgc
     1621 cccgcatccc gagccgcacg cggcgccgca ggagcctgca cgccgcacgg gcgagtcgcc
     1681 cgcgcgcgcg ctcgaagggc tgcgcgtgct gctcgtggac gaccacgagg acacgctgca
     1741 actgctctcc atgtttctgc gcaggcaggg cgcacaggtc agggcggttg actccgccgc
     1801 gcgcgccctc gaagagatcg cggaggcgcg gcccgacgtg ctcgtcagcg acatcgggat
     1861 gcccggcgag gacggctacg agctgatgcg gaaggtgcgc gcgctggcgc cggggcgggg
     1921 cgggcgtacg cccgcggtcg cgctgacggc gtacgccggt gaggccgacc gccggctcgc
     1981 cttcgaggcc gggttcgacg cgcacgtggc gaagccggtc gagccggagg cgctcctctc
     2041 gacgctcgcc gacctcgcgg ggaggtgaag acccaaaggc tcgacgggag tgccggtgag
     2101 gcttaggcaa aggccggcgg gcatcgtcgg aaggacggac ggcgaatagc ggatggcgac
     2161 atgagcgcgg cgacgcctgc cggcgtgcgg agtgctctca tgctgttcgg tctgtcaggg
     2221 tctcacttta cgggaaacct gccgaaccct cttcgtcggt cgtgtcgaag ttgggcggcg
     2281 cccgaagctc tccctcttcg aagaagatct cccgcgccgc tttgacctga atcttctgct
     2341 tcggcgcgcg cccgccgccg acctcgcccg ccgcctcttc gccctccttg agcctcgaag
     2401 cgaccgaatt ctccctgtcg ttgaagacca cgcgcacgcc cttgccgagc tgcgcgtcgg
     2461 tgaacttgtg gccgggcagc tcgacggcga ccatctggtc gaagtcgggc ggactcgtga
     2521 tgaggtgggc catgaaaagc tcgccgccct tgccgaagac caagtattcc agcttgccga
     2581 gccgcgcggc cttcgggtcg aactcgtgga agtggacgac gttcttcacc gtcacatcga
     2641 cgttgtcgag gacggcgacc ttgccgtctc tttcgaagtg gccgcggaag accgtccctc
     2701 tgaacgtgcg gcgcgggcgg ctggcgacga gttcggcgag cttgaacgtc tccctcggat
     2761 tgagcatgta gattttcgta tcccgatgct gcgtgcggtc tgcggcgtag tcggtctgcg
     2821 gctgcgcgct gctgccgcgc atgaaggccg cttccagcac gacctgaaaa cggtgcggcg
     2881 tggcgacgag gttgccttcg gcgtcctcct ctttgaacat cggcaggtgc gagaggaaga
     2941 cggtcttctc gccgacgacc agcatgttgt gcgtggcgat gtcgcccctg tgctggtgcg
     3001 gcgcggccag cgctttctgc ccccgcgctt cgcggagaaa cgccgtcccc acgagcgccg
     3061 ccgcgccccg cgtcagaaag cccctgcgcc cgtaaccgtc gctgctgttt tcggacatcc
     3121 gacacacatc ctttggttag ccgagggtcg tgaagcggcg aggcgaaacg ttcgctcgga
     3181 cgcgtcgaac gatatgcccg agagggcgaa gtgtcaacgg cgagtcggcg gcaggccgtc
     3241 ggcggggcga acggggccgc ggcggcacac gcatcgttga cacgcggccc cctcgccgca
     3301 taccttagcc gcctcccgcg cgctccgcag tgcatcccgt ttcggtttgg attagactag
     3361 gctctgctgc tcaatccccg atcatgaaag tgattcccga aaccaccacc atcgcgcatt
     3421 accggatcat ggagtcgctg ggcgcgggcg gcatgggcgc ggtcttcaag gcgtacgacg
     3481 aaaagctgca ccgcgtcgtc gcgctcaagc tcctgccgcc cgaatacacc tcgcacgaag
     3541 accgccgccg ccgcttcctg caagaggccc gcgccgcctc ggcgctgaac cacccgcaca
     3601 tcctcaccgt ctacgagttc ggcgaggacg gcgggcggcc ctacatggcg atggagtacg
     3661 tcgagggcga gacgctgcgg cagaggatcg cggcgagccc cctacagctc ggcgagggtc
     3721 tggggatcgc ggtgcaggtg gccgaggggc tggccaaggc ccacgcggcc ggcatcgtcc
     3781 accgcgacct gaagccggag aacctgatga ttgccgacgg gggctatgcc aaggtcctcg
     3841 acttcggcct cgccaagctc aacgcggaga cgctgtccgc gaacatcgcg accgatgcga
     3901 cccgcaccgt ggcgagcccc gccgggacga accccggtca ggtgatgggg acggtgggct
     3961 acatgtcccc cgagcaggca caaggtctcc cggtcgatca tcgctccgac atcttttcct
     4021 tcgggtgcat cctgtacgaa tcggcgaccg gggc
//
LOCUS       DBNGOH010000164         3712 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2589832, whole genome shotgun sequence.
ACCESSION   DBNGOH010000164 DBNGOH010000000
VERSION     DBNGOH010000164.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3712)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3712)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3712
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2589832"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1180
                     /locus_tag="AC159P_03010"
     CDS             <1..1180
                     /locus_tag="AC159P_03010"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024968.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR3694454.1"
                     /translation="GVSLLTFEPELKERWTAEDLNDERSRLEADGIRWLALPYHKRPS
                     VPATVYDIFAGAWFAARHARREGVEVLHARAHVPAAMAMLARVLVPRLRFVFDIRGLM
                     AEEYADAGVWAKGSLPFRAVKWLERAAIRRADRIVVLTRVMRDWLVKEKLAPAGKVEV
                     IPCCVDFSRYAQDAGGKAAESEKGEEASAKNERFEVVYAGSATGLYMLEEMGRFFAEL
                     RALRPGAFFRVLTMSPPAEVQRVLREAGLKGEEFHVGRASASEVPAVLRGARLGVSFR
                     KATFSQIAASPTKIPEYLAAGLPVVSNAGIGDTDEVLEPEAVGVLVQRFDRESYARAA
                     EEARALADDPSTPARCLAAARRHFDLAGVGGARYLNVYRRLTTEVGNQKSEVRSREST
                     "
     gene            1257..2381
                     /locus_tag="AC159P_03015"
     CDS             1257..2381
                     /locus_tag="AC159P_03015"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR3694455.1"
                     /translation="MRLLAVVPSVYDRNPSQRYRIEQWEPHLRRMGVEITYRPFEDEE
                     LNRVLYQPGRMPRKAGLILKALARRFGDVREARAFDAVYILREAALLGPPVFERMIGF
                     AGVPFVFDFDDAVFERYVSPSNGYLSYLKFPGKTRTVCRLAAHVMAGNEYLADYARAV
                     NGRVTVIPTTVDTEEYTVEERAPNEVPVVGWTGSYSTVQHLLRLAGALRRLAERERFR
                     LRVIGAPNMSLAELAGVETEVLPWRSETEVADLRPVDVGVMPLPDDPWSRGKCGMKAL
                     QYMGLAIPTVWSPVGVNSAIIRDGVNGLLASTEDEWVEKLALLLRSPSERARLGHAGR
                     ATVEATYSAAVQAPRVREIIESVVTPGVKTSGGPRGRSES"
     gene            2405..3133
                     /locus_tag="AC159P_03020"
     CDS             2405..3133
                     /locus_tag="AC159P_03020"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HIR3694456.1"
                     /translation="MSRRSSKFRLAALALVALLPSFLKRPAYRLCFGYKIGKRVRVGL
                     SLIDADACEIGDDVRVGHLNVFTGIKELTVGEHARVGHLNVIRGGDEVRIGRWAEIIR
                     LNEINSIPDPVVVNPVEPKFFLGDGGIVTAGHKIDFTDRVEIGRRTILGGRNSSLWTH
                     NRQRTRPILIGELSYIGSEIRVAPGGVIPSRCIVGIGSVITGRLEGENQLIAGVPAKP
                     IKPLSEEDRFLIENKTRPDLPDEI"
     gene            3327..>3712
                     /locus_tag="AC159P_03025"
     CDS             3327..>3712
                     /locus_tag="AC159P_03025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694457.1"
                     /translation="MNALLIALALAMSLGVAFVAHPYGAGAVLVCSVLAVPVALVISQ
                     SEHKIFLLRVFAAALFARMVVGTAIHLFDLQVFFGADAYTYDELGRVLAQVWAGGSGY
                     IGPEELLGPFLERNWGMIYVVAGIYTA"
BASE COUNT          581 a         1268 c         1253 g          610 t
ORIGIN      
        1 gggcgtgagc ctgttgacgt tcgagccgga gttgaaggag cgttggacgg cggaagattt
       61 gaacgacgag aggtcgcgtc tcgaagccga cgggattcgc tggctcgcgc tgccgtacca
      121 caagcgaccg tcggtgcccg cgaccgtgta cgacatcttc gcgggcgcgt ggttcgcggc
      181 gcgtcacgcg cggcgcgaag gggttgaagt gctgcacgcg cgcgcgcacg tgcccgcggc
      241 gatggcgatg ctggcgcgcg tcctcgtgcc gcgcttgagg ttcgtgttcg acatccgcgg
      301 cctgatggcc gaggagtacg cggacgcggg cgtgtgggcg aaggggtctc tgccgttccg
      361 cgcggtgaag tggctggagc gcgcggccat ccggcgcgcg gatcgcatcg tcgtgctcac
      421 gcgcgtcatg cgcgactggc tggtgaagga aaaactcgcg cccgccggga aggtcgaagt
      481 catcccctgc tgcgtggact tctcgcgtta cgcgcaggac gcgggaggga aggcggcgga
      541 gtcggaaaag ggcgaagagg cttcggcgaa gaacgagcgg ttcgaggtcg tctacgcggg
      601 ttcggcgacg ggcctctaca tgctcgaaga gatggggcgc ttcttcgcgg agctgcgcgc
      661 gctcaggccc ggcgcgttct tccgcgtgct gacgatgtcg ccgcccgcag aggtgcagcg
      721 cgtgctgcgc gaagcgggtc tcaagggcga ggagttccac gtcggcaggg cgagcgcgtc
      781 ggaagtgccc gccgtcctgc gcggcgcgcg gctcggcgtc tctttccgca aggccacctt
      841 ctcgcagatc gcggcctcgc cgacgaagat tccggaatac ctcgccgccg ggctgcccgt
      901 cgtcagcaac gcgggcatcg gcgacacgga cgaagtgctc gaacccgagg cggtcggcgt
      961 cctcgtgcag cgcttcgacc gcgagtctta cgcgcgcgcc gccgaagagg cgcgcgcgct
     1021 cgccgacgac ccctcgacgc ccgcgcgctg ccttgccgcc gcgcgcaggc acttcgacct
     1081 cgcgggcgtc ggcggcgcgc ggtacctgaa tgtttaccgc cgcctgacaa cagaagtcgg
     1141 aaatcagaag tcagaagtca ggagccggga gtcaacatga aaaactcccc ccttccgact
     1201 cgtccttctg attgcttctt ccgacttccg atttccgact tccgacttct atcgttatgc
     1261 gtcttctcgc cgtcgtcccc agcgtctacg accgcaaccc gagccagcgc taccgcatcg
     1321 agcagtggga gccgcacctg cggcgcatgg gcgtggagat cacgtaccgg cccttcgagg
     1381 acgaagagct gaaccgcgtg ctctatcagc cgggccggat gccgcgcaag gcggggctga
     1441 ttctgaaggc gctcgcgcgg cgcttcggag acgtgcgcga ggcccgcgcc ttcgacgccg
     1501 tctacatcct gcgcgaggcg gcgctgctgg ggccgcccgt cttcgagcgc atgatcggct
     1561 tcgcgggcgt gcccttcgtc ttcgacttcg acgacgccgt cttcgagcgc tacgtcagcc
     1621 cctcgaacgg ttatctgagt tacctgaagt tcccgggcaa gacgcgcacc gtgtgccgcc
     1681 tcgccgcgca cgtgatggcg ggcaacgagt acctcgccga ctacgcgcgc gcggtgaacg
     1741 ggcgcgtgac ggtcatcccg accaccgtcg ataccgaaga gtacacggtc gaagagcgcg
     1801 cgccgaacga ggtgcccgtc gtcggctgga cgggcagcta cagcaccgtc caacacctgc
     1861 tgcggctggc tggcgcgctg cgccgtctgg cggagcgaga acgttttcgg ctgcgcgtga
     1921 tcggcgcgcc gaacatgagc ctcgcggagc tggcgggcgt cgagaccgag gtgctgccgt
     1981 ggcgctcgga gacggaggtc gcggacctgc ggcccgtgga cgtgggcgtg atgccgctgc
     2041 cggacgaccc gtggagccgc ggcaagtgcg gcatgaaggc gctccaatac atgggcctcg
     2101 ccatcccgac cgtctggtcg ccggtcggtg tcaactctgc tatcatccgc gacggcgtca
     2161 acggcctgtt ggcctcgacc gaggacgagt gggtcgagaa actcgcgctg ctgctccgct
     2221 cgccgtccga gcgcgcgcgg ctcggccacg cggggcgcgc caccgtcgaa gccacctact
     2281 ccgccgccgt gcaggcgccg cgcgtccgcg agatcatcga gtcggtcgtc acccccggag
     2341 tgaaaacgag cggcgggccg cgaggccgaa gcgagtcgtg aggcgcgggt tcgattggta
     2401 aagcatgtca cgtcgcagta gtaaattccg cctcgcggcg ctcgcgctcg tcgccctgct
     2461 gccgagtttc ctgaagcgcc cggcctaccg gctctgcttc ggctacaaga tcggcaaacg
     2521 cgtgcgcgtc ggcctcagcc tcatcgacgc cgacgcgtgc gagatcggcg acgacgtgcg
     2581 cgtcggccac ctcaacgtct tcacgggcat caaggagttg accgtcggcg agcacgcgcg
     2641 cgtcggccac ctcaacgtca tccgcggcgg cgacgaggtg cgcatcgggc gctgggccga
     2701 gatcatccgc ctcaacgaga tcaactcgat ccccgacccc gtcgtcgtca acccggtcga
     2761 gccgaagttc ttcctcggcg acggcggcat cgtcaccgcc ggccacaaga tcgacttcac
     2821 cgaccgcgtc gagatcgggc ggcgcaccat cctcggcggg cgcaactctt ccttgtggac
     2881 gcacaaccgc cagcgcacgc gccccatcct gatcggcgag ctgagctaca tcggctccga
     2941 gattcgcgtc gcgccgggcg gcgtgatacc atcccgctgc atcgtcggca tcggctccgt
     3001 catcaccggg cgcctcgaag gcgagaacca actcatcgcg ggcgtcccgg cgaagcccat
     3061 caagccgctc agcgaagaag accgcttcct gatcgagaac aaaacgcgcc ccgacctgcc
     3121 ggacgagata tgacgaagca ggcaccagac acttaggcgg taggcagtca agacggttga
     3181 gtccgagtcc cttgcatccg taacccttca aacctgattg gttccgaacc cttcgttaac
     3241 ttgagcgcgg caaccgaacg agaacctcgc gcggaggcgg tttttgaact gcctaccgcc
     3301 taccgcccac cgcctactgt tccccaatga acgcccttct tatcgccctt gcgctcgcca
     3361 tgtcgctcgg cgtcgcgttc gtcgcgcacc catacggggc gggggccgtg ctggtctgct
     3421 cggtgttggc cgtgcccgtc gcgctcgtca tcagccagag cgagcacaag atttttctgc
     3481 tgcgcgtgtt cgccgccgcg ctgttcgcgc ggatggtcgt cggcacggcc atccacctct
     3541 tcgacctaca ggtcttcttc ggcgcggacg cctacaccta cgacgaactc ggcagggtgc
     3601 tcgcgcaggt ctgggcgggc ggctccggct acatcggccc cgaggagctg ctcggcccgt
     3661 tcctcgaacg caactggggc atgatctacg tggtcgccgg gatttacacg gc
//
LOCUS       DBNGOH010000165         5478 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2039921, whole genome shotgun sequence.
ACCESSION   DBNGOH010000165 DBNGOH010000000
VERSION     DBNGOH010000165.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5478)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5478)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5478
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2039921"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..834
                     /locus_tag="AC159P_03030"
     CDS             <1..834
                     /locus_tag="AC159P_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002620863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter domain-containing
                     protein"
                     /protein_id="HIR3694458.1"
                     /translation="LNVLAFVLVGFQLKSIAARATGATGARYAAVAAAVCAAVILARI
                     AWVSGAAAFSRWCCRPLADGAAGPRDAVALSARGAAVVGWCGMRGTVTLATALALPTD
                     FPYRDLILTTAFGVTLGTLVLQGLTLRPLLLRLRLEDDGSVDREVRLARVEALRAALE
                     AAAACPGAETAALVRHSYELQLRRAEEEASGGGVKMGEASPKVPAATAPTAACTDGAE
                     ADAGVLRAATEAQRRRLVALRDDGTIGDAAFQRVEEELDWAELGWAQLDRTGRRTDGA
                     A"
     gene            875..1099
                     /locus_tag="AC159P_03035"
     CDS             875..1099
                     /locus_tag="AC159P_03035"
                     /inference="COORDINATES: protein motif:HMM:NF015747.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HIR3694459.1"
                     /translation="MRIGVLGSGLMGGKLGTIFTRAGHEEVFSYSRSEQKLKNLARNA
                     GGNARAATPGEAAKGADALLLAVHWSGEQA"
     gene            complement(1246..2142)
                     /locus_tag="AC159P_03040"
     CDS             complement(1246..2142)
                     /locus_tag="AC159P_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011549366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HIR3694460.1"
                     /translation="MAALVSAHAPSDGRFELHVPGVSAVRFSQPSAHLVHAVVGPVMC
                     VVAQGAKSVMLGREVYEYDASRMLIFSVDLPVTGQVTRASRSEPFLCLRMELDPRRIA
                     ELALKVYPDGLSQIQEGRGVYVTQADGGIVNAAARLVELAADPADAELLAPLVVDELL
                     IRLLRSRAGGRVAQIGLANSSMHRIAKAISWLRANYARPLKVEELAGLTNMSVSSFHQ
                     HFKSFTSMSPLHYQKVLRLQEARRLMLSSTLDAAAASRQVGYLSASQFSRDYGRFFGS
                     APARDVARLREQGVAAAAGVSR"
     gene            2629..2952
                     /locus_tag="AC159P_03045"
     CDS             2629..2952
                     /locus_tag="AC159P_03045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694461.1"
                     /translation="MDFIGIDLHKNSSQVCILTEDGELVERRIKTDRESFDKLLGERP
                     LARILVESSTESEWCARHLETLGHEVIVADPNFAPMYATRDRRALHRSSGLLTPLPPL
                     FGTWV"
     gene            complement(2895..3041)
                     /locus_tag="AC159P_03050"
                     /pseudo
     CDS             complement(2895..3041)
                     /locus_tag="AC159P_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009769460.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(3137..3808)
                     /locus_tag="AC159P_03055"
     CDS             complement(3137..3808)
                     /locus_tag="AC159P_03055"
                     /inference="COORDINATES: protein motif:HMM:NF012976.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dioxygenase family protein"
                     /protein_id="HIR3694462.1"
                     /translation="MREPFSRRRVLKLVLAWGALALGAPLHSVFAQGQRLKESPNTVV
                     GPFYPLIKPLDQDADLTVIAGGSGRAEGKILHLTGRVLNRGGEPVRGARVEIWQADSR
                     GRYAHTSDRGTPTRDLKFQGFGVQTTDAEGRYRFKTIKPAPYSSEGGSLRAPHIHFDV
                     SGRADRKVTQMFFEGEPLNDQDRIFQATLRPVWASAVGKAQPPGRGMEPDSLLVIWDV
                     VLDHG"
     gene            complement(4049..4822)
                     /locus_tag="AC159P_03060"
     CDS             complement(4049..4822)
                     /locus_tag="AC159P_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015190271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR3694463.1"
                     /translation="MINYGLAGKVALVTGVSRRAGIGAAVARAFAEAGADVFTTYYRP
                     YDELMPWGSDAGEAEDIVRGLKEKGVRAEGVEADLADPEVPARLFAHVRGSFGFVDIL
                     VNNAVCDMGADVYGLSAFLIDRHYAVNVRAAMLLCAEFAKCHDGRRGGRIVNMSSGQG
                     AGPMPDNLPYAATKGAVEAFTLSMSASLAGKGITVNAIDPGPTDSGWLSDDLRSELLQ
                     RAPFGRIGTPDDAAKLVLYLASSEAQWITGQLIRSRGGL"
     gene            complement(4943..>5478)
                     /locus_tag="AC159P_03065"
     CDS             complement(4943..>5478)
                     /locus_tag="AC159P_03065"
                     /inference="COORDINATES: protein motif:HMM:NF012976.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dioxygenase family protein"
                     /protein_id="HIR3694464.1"
                     /translation="GPYYPVIRPLERDSDLTRLGGHRSRAKGQVIHVAGRVLNQRGEP
                     LRGARVELWQANAAGRYSHPSDPNPAPLDPDFQGYGAQMTDREGRFRFTTIKPGPYPF
                     EGGTRAPHLHFQVTGRAETRVTQMFFAGEELNVQDQVLRCVARRRESMVADLLPAPPD
                     EDPATRLVRWDIVLRHG"
BASE COUNT          927 a         1824 c         1831 g          896 t
ORIGIN      
        1 ctcaacgtgc tcgcgttcgt cctcgtgggc ttccaactca agtccattgc cgcgcgcgcg
       61 accggggcga ccggggcgcg ctacgcggcg gtggcggcgg cagtatgcgc cgcggtgatc
      121 ctcgcgcgca tcgcgtgggt gagcggcgcg gcggccttca gccgttggtg ctgccggcct
      181 ctggccgacg gggcggcggg gcctcgcgac gccgtggcgc tcagcgcgcg cggcgctgcc
      241 gtggtgggtt ggtgcggcat gcgcgggacc gtgacgctcg ccaccgcgct cgcgctcccg
      301 accgacttcc cctaccgcga cctgatcctg acgacggcgt tcggcgtcac cctcggcacg
      361 ctcgtgcttc aggggctgac gctgcggccg ctcctgctgc gcctgcgact cgaggacgac
      421 ggctcggtag accgcgaggt gcgtctcgcg cgggtcgagg ccctgcgggc ggcgcttgag
      481 gcggcagcgg cctgcccggg ggccgagacc gcggcgcttg tccggcacag ctacgaacta
      541 caactgcgac gggcggagga ggaagcgagc ggaggcggcg tgaagatggg cgaggcttcg
      601 cccaaggttc cggcggctac ggcgccgact gccgcgtgca cagacggcgc ggaagccgac
      661 gcgggggtct tgcgggccgc gaccgaggcc caaaggcggc gcctcgtggc actccgcgac
      721 gacggcacga tcggcgacgc cgcgttccag cgtgtcgagg aagaactcga ctgggcggaa
      781 ctcggctggg cgcagctcga ccggacgggg cggcggacag acggggcggc gtagccaccg
      841 gacgccgggg tgaacgtcag ggaggagata cggcatgcgc ataggcgtcc ttgggtcggg
      901 gttgatgggc gggaagctcg ggacgatctt cacgcgggcg gggcacgagg aggtcttcag
      961 ctactcacgc agcgagcaga agctgaagaa cctcgcgcgc aacgcaggtg ggaacgcgcg
     1021 ggcggcgaca ccgggtgagg cagcgaaggg cgcggacgcc ctcctgctgg ccgtccactg
     1081 gtccggggag caagcataac gcgggaccgc gttgctcttt tgcccgatgg caccctgggc
     1141 gtcggactcg acgtgcgccg tcaccttact ctagtgtgat aaatggtgtc gcctcggcgc
     1201 aagcgaccct gccgggaggt cgccgagcgg ggcttcgtcg gcggtctacc gggaaacgcc
     1261 cgccgccgcc gcaacgccct gctcacgcag cctggcgaca tccctggccg gcgcgctccc
     1321 gaagaagcgg ccgtaatccc tgctgaactg cgaagcgctc aggtacccca cctgccgact
     1381 cgcggcagcc gcgtccagag tggaggacag catgaggcgc ctcgcctcct ggaggcgcag
     1441 gaccttctgg tagtgcaggg ggctcatcga ggtgaatgac ttgaagtgct ggtggaagga
     1501 ggagacgctc atgttcgtca gcccggccag ctcttcgacc ttcaagggcc gggcatagtt
     1561 ggcgcgcagc cacgagattg ctttcgcgat cctgtgcata ctcgagttcg cgaggccgat
     1621 ctgggcgacc cttccgccgg cgcggctgcg cagcaggcgg atcagaagct catcaactac
     1681 gagcggcgcg agcagttcgg cgtccgccgg gtcagccgcc aactcgacca gcctcgccgc
     1741 ggcgttgact attccgccgt cggcttgggt gacgtacacg ccgcgcccct cttgaatttg
     1801 agacaggccg tccggataca ccttcagagc cagttcggcg atcctgcgcg ggtcaagctc
     1861 catcctgaga cagaggaaag gctcggagcg gctggcccgc gtgacctggc cggtgaccgg
     1921 caggtcgacg gagaagatga gcatgcgcga cgcgtcgtac tcgtagacct cccgcccgag
     1981 catcacgctc ttcgccccct gcgcgacgac gcacatgacc ggccccacca cggcgtgcac
     2041 caagtgggcg ctcggttgcg agaagcgaac ggcggacaca ccggggacgt gcagctcgaa
     2101 gcgtccgtcg gaaggggcgt gggcgctgac caacgcggcc aacttacgca agtctgtttc
     2161 gctcggcgcc gcgacggcgt ccgcttgcgg cgggggcggg gtgttgtcag tagacttcaa
     2221 aacaggcctc cggtcagcta caagcatcct ttctcaagaa tggcccctgc gcgaggatga
     2281 tggcggatgc tacataatat aacgtcccgc cagtcgtatt aaaccctcgg ccgccatctg
     2341 ccacccgttt agcgaccagc cttaacaagg ccctctacaa aaatgccaag ttaagaatcg
     2401 gggaatttgt gtttcgtaaa ggacgttaag attcatcaac tcacgtcgcc cttaactcgc
     2461 cttctttcta ccttcgtaga cttccggact caaaggtagg attcgctgtc atccagtttt
     2521 gcgatgggtt cacaacggaa ttggatgaag cggctggtcg ccacctcggt ggtgagataa
     2581 tgctccacac actttgcggc ttatgaccgc aggaaggagt caccagccat ggactttatc
     2641 ggcatcgacc tccacaagaa ctcaagtcag gtctgcatcc tgaccgagga cggcgaactc
     2701 gtcgagcgcc gcatcaagac cgaccgggag agcttcgaca agctcttggg tgagaggccg
     2761 ctggctcgta tcctcgtcga gtcttcgacc gagagcgagt ggtgcgcacg ccaccttgaa
     2821 acacttgggc atgaagtaat cgtcgccgac ccgaacttcg cgccgatgta cgccacccgc
     2881 gaccggcgcg cccttcaccg ctccagcgga ctcctcacgc ccctgccgcc cttgttcggg
     2941 acgtgggtat agatccgcgt cgtccgcacg cccgagtgcc ccggaagctc ctggatcgtc
     3001 cgcggatcgt agccgtcttc gagcaggtgc gtgccgttgc tctgccgccg gacgggacaa
     3061 ctcaaggatg aaagaattgc caccccgctc ccaggttcag agagttaccg ggcgcgcggc
     3121 cgagggcagg ggagggtcag ccatggtcga gtaccacgtc ccagatgacg agcagcgagt
     3181 ccggctccat gccccgcccg ggcggctggg ccttccccac cgcgctcgcc cagacggggc
     3241 ggagcgtggc ctggaagatg cggtcctggt cgttcagcgg ctcgccctcg aagaacatct
     3301 gcgtcacctt gcggtccgcc ctgccgctca cgtcgaagtg gatgtgcggc gcccgcagcg
     3361 agcccccctc ggacgagtac ggcgcgggct tgatcgtctt gaaccggtag cgcccctccg
     3421 cgtccgtcgt ctgcaccccg aacccctgga acttcaggtc gcgcgtaggg gtgccgcggt
     3481 cggacgtgtg cgcgtagcgg ccgcggctgt cggcctgcca gatctcgacc cgcgcgccgc
     3541 ggacgggctc cccgcccctg ttgagcaccc tccccgtcag gtggaggatc ttaccctcag
     3601 ccctcccgga cccgcccgcg atgacggtca ggtcggcgtc ctggtcgagg ggcttgatga
     3661 gggggtagaa cgggcccacc accgtgttgg gtgattcctt gagcctctgc ccctgggcga
     3721 agacggagtg gagcggggcg cccagcgcga gggcgcccca cgcgagcacc agcttcagga
     3781 ctcggcggcg cgaaaagggt tcccgcacgt cagtcatttc agttactccc gcttcaggtg
     3841 gcgcccgaag ccgacacaac gaagagcaag ggtgaaaagg tggccaaaat attaccgccg
     3901 agccttcgtg tctacgcgcg ctcgttcaag caagcgccga acgctggtgt tcagcggcgc
     3961 gccgaggtca tcgaattcaa gagcggcgcc gattgcgcgt ccgcggcaac accttgtcgt
     4021 gctgccccgc gggcggcact tccgagcttc ataagcctcc cctcgaacgg atgagctgcc
     4081 cggtaatcca ctgcgcttct gaagaggcaa ggtagagaac taacttggcc gcatcatcag
     4141 gagtgccgat gcggccgaac ggcgcccttt gcagcaactc cgagcgcaaa tcatcgctca
     4201 gccacccgct atccgtcgga cccggatcaa tcgcgttcac ggtgatgccc ttgcccgcga
     4261 gcgacgcact catgctcaac gtgaaggctt caaccgcccc tttagtcgca gcatacggca
     4321 agttgtccgg catcggcccg gcgccctggc ctgaagacat gttgacgatg cgcccgccgc
     4381 gccgcccgtc gtggcactta gcgaactcgg cgcaaagcag catcgccgcg cgaacattca
     4441 cggcgtagtg cctgtcgatc aaaaaagcag ataggccata aacatccgcc cccatgtcgc
     4501 agacggcatt attaacaagg atatcgacga agccgaatga gccgcggacg tgagcgaata
     4561 accgggccgg gacttccggg tcggcgaggt cagcctccac cccttcggcg cgcacgcctt
     4621 tttcttttag ccctctgacg atgtcttcgg cttcgcccgc gtcactaccc cacggcatga
     4681 gttcgtcgta agggcggtag tacgtggtga acacatcggc gcccgcttcg gcgaaagcgc
     4741 gtgcaacagc cgccccgatc ccggcccgtc tactcactcc ggtcactaac gcaactttgc
     4801 ctgcgagccc gtaattaatc atcgaattga accttgacca tttataggca gcacaacgcc
     4861 tggcatctca cctgcccgcc cgcaagattg attgacatga gagtcgccgt gtcgcgggtc
     4921 gggcgcattg gcttgttcgg ggtcacccgt gacgcaggac gatgtcccac cggacgagcc
     4981 gtgtggccgg gtcctcgtcc ggcggcgcgg gcaggagatc ggccaccatg ctctcgcgcc
     5041 ggcgcgcgac gcaccgcagg acctggtcct ggacgttcag ctcctcgccg gcgaagaaca
     5101 tctgcgtgac gcgcgtctcg gcccgccccg tcacctggaa gtggaggtgc ggcgcccgcg
     5161 tgccaccctc gaaggggtac ggcccgggct tgatcgtcgt gaagcggaac cgcccctcgc
     5221 ggtccgtcat ctgggcgccg tagccctgga agtccgggtc gaggggcgcg ggattcgggt
     5281 cggaggggtg gctgtagcgg cccgcggcgt tggcctgcca gagttcgacg cgcgcccccc
     5341 gtagcggctc accccgctga ttcaggacgc gcccggccac gtgaatgacc tgacccttgg
     5401 cgcgcgagcg gtggccgccg aggcgcgtca ggtccgagtc ccgctcgagg ggcctgatga
     5461 cggggtagta agggccga
//
LOCUS       DBNGOH010000166         1673 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3324050, whole genome shotgun sequence.
ACCESSION   DBNGOH010000166 DBNGOH010000000
VERSION     DBNGOH010000166.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1673)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1673)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1673
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3324050"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            81..>1673
                     /locus_tag="AC159P_03070"
     CDS             81..>1673
                     /locus_tag="AC159P_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HIR3694465.1"
                     /translation="MSHTPPDGRTLSELNERVSAARAETEARGETFYPGPSRIHLAAF
                     PPKERWDDWVELDSRAWPRREERRYMLVPTTCFNCESACGLLAYVDKESLSVRKFEGN
                     PEHPGSRGRNCAKGPATLNQITDPDRILYPLKRAGRRGEGKWVQVSWDEALDDLAARI
                     RQSITEGRANEVMYHVGRPGEDGFTERIMAAWGVDGHNSHTNVCSSNSRAGYQYWMGL
                     DRPSPDHANAKVIFLISSHLEAGHYFNPHAQRVIDGKAAGAKLIVFDTRLSHTATHAD
                     FWVAPYPGSEAAIVLSIASYLIKHRLYDREFVRRWWNWEEFLAVECPEVETTFENFEH
                     VLRMLYESYTFEFAARESGVEAATIEEIAKVVSTAGSRFSSHNWRSAAAGNSGGWQVA
                     RTLFFLNALLGAVATEGGVYPNAWNKYVPKPIHTPPHPPAWNELTWPREYPLAMNELS
                     FLLPHFLKEGRGRLEVYFTRVYNPVWTNPDGFSWIEALTDESLVGCHVALTPVWSETA
                     YFADYVLPMGLGSERHDLHSYET"
BASE COUNT          292 a          548 c          569 g          264 t
ORIGIN      
        1 ccgatctggc cgcgatcgcg ttcctcggct cgctcgacat caacaactga ccgccgcaca
       61 cggtcgcacg aagaagcagc atgagccaca caccaccaga cggtcgcacc ctctcagagc
      121 tgaacgagcg cgtgagcgcc gcgcgcgccg agacggaggc gcggggcgag actttttatc
      181 cggggccgag ccgcatccat ctggcggcct tcccgccgaa ggagcgctgg gacgactggg
      241 tcgagcttga ttcgcgcgcg tggccgcggc gcgaggagcg gcgctacatg ctggtgccga
      301 cgacgtgctt caactgcgag tcggcgtgcg ggctgttggc gtacgtggat aaagagtctc
      361 tgagcgtgcg gaagttcgag gggaaccccg agcaccccgg ctcgcgcggg cgcaactgcg
      421 cgaaggggcc tgcgacgttg aatcagatca cagaccccga ccgcatcctc taccctctga
      481 agcgcgcggg ccgtcgcggc gagggcaagt gggtgcaggt ctcttgggac gaggcgctgg
      541 acgatctggc ggcgcgcatc cgccagagca tcaccgaagg gcgcgcgaac gaggtgatgt
      601 accacgtggg ccgaccgggc gaggacggtt tcaccgaacg catcatggcg gcgtggggcg
      661 tggacgggca caactcgcac acgaacgtct gctcttcgaa cagccgcgcg ggctaccaat
      721 actggatggg cctcgaccgc ccgagtccgg accacgccaa cgcgaaggtc atcttcctca
      781 tcagctcgca cctcgaagcg ggccactact tcaacccgca cgcgcagcgc gtgattgatg
      841 ggaaagccgc gggcgcgaag ctcatcgtct tcgacacgcg gctgtcgcac acggcgacgc
      901 acgccgactt ctgggtcgcg ccgtatccgg gcagcgaggc ggccatcgtg ctctcgattg
      961 cgagctacct gatcaagcac aggctttacg accgcgagtt cgtgcgccgc tggtggaact
     1021 gggaggagtt cctcgccgtc gagtgccccg aggtcgagac gacgttcgag aacttcgagc
     1081 acgtgctgcg gatgctctac gagagctaca cgttcgagtt cgcggcccgc gagtcgggcg
     1141 tcgaggcggc gaccatcgag gagatagcga aagtcgtctc gacggcaggc tcccgctttt
     1201 cgagtcacaa ctggcgctcc gccgccgcgg gcaacagcgg cggctggcag gtggcgcgca
     1261 cgctgttttt cctgaacgcg ctgctgggcg cagtggcgac cgagggcggc gtctacccga
     1321 acgcgtggaa caagtacgtg ccgaagccga ttcacacgcc gccgcacccg cccgcgtgga
     1381 acgagctgac gtggccgcgc gagtaccctc tggcgatgaa cgagctgtcg ttcctgctcc
     1441 cgcacttttt gaaggagggg cgcggcaggt tggaggtcta cttcacgcgc gtctacaacc
     1501 ccgtgtggac gaaccccgac ggcttctcct ggatcgaagc tttgaccgac gagtcgctgg
     1561 tcggctgtca cgtcgcgctg acgcccgtgt ggagcgagac ggcctacttc gccgactacg
     1621 tcttgccgat gggcctcggc tccgagcgcc acgacctgca ttcctacgag acg
//
LOCUS       DBNGOH010000167         7730 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_24313, whole genome shotgun sequence.
ACCESSION   DBNGOH010000167 DBNGOH010000000
VERSION     DBNGOH010000167.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7730)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7730)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7730
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_24313"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1848
                     /locus_tag="AC159P_03075"
     CDS             <1..1848
                     /locus_tag="AC159P_03075"
                     /inference="COORDINATES: protein motif:HMM:NF015862.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlcG/HbpS family heme-binding protein"
                     /protein_id="HIR3694466.1"
                     /translation="EHVGPVPGQEWLHSVTLRFDDTMGDVGDVLVRLIYKGVASNRVR
                     VGVGHVGGGPPDDAGAVPTPVLALAMPTPNANPVTAGNLTTDDVRQVIAQAVSAAVAM
                     NRRVTVAVTDREGNVLGVFSMTGAPTQTQFRGATGNDMTPNPITGLVPRGLAGTNLPP
                     VLIPVPRAAISKAGTASFFSTFGNAFTPRTASFIIQEHFPPSVNFQPGGPLYGVQFSQ
                     LPCSDIKTPTLPFGLSGDPGAVPLYKNGVPSGGVGIEGDGVYTIDRDVTDFDKPFEEV
                     IAVAAGRGYEPPGLIRGDNIIVGGVRLPYVNVTDEDAPRPATIAFGSLPGQIDPMFPI
                     RGAQPSAFVATNVGGVNGAADTRFFPFISSPSASSNRLTAADVSTIIGNAARQADITR
                     AAIRQPLGSQARVSITVVDIEGRVLGIFRTTDAPVFGFDVSAQKARSSVFISRPDAGT
                     LLRAVGMGAYVDRAAADGLGLNGAIAFSDRAFGFLHRPFFPDGLSGTPAGPFSTEIGE
                     WSVFNLGLQTDAITTNLVAALVGANVRCTALPQVPNGFQIFAGGIPLYKNGELVGAIG
                     ISGDGIDQDDIIAAAGANGYAPPAAMRSDQTIVRDTRLPFVKFPRSPNL"
     gene            2084..3346
                     /locus_tag="AC159P_03080"
     CDS             2084..3346
                     /locus_tag="AC159P_03080"
                     /inference="COORDINATES: protein motif:HMM:NF025585.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4331 family protein"
                     /protein_id="HIR3694467.1"
                     /translation="MLRPNNKSVALVALALTAALALLLTPAPRGSAADHGDAPFVAND
                     RGADIADTYIFRDPVDNSRVVIAGTINGFIVPNEMVNFGVFDENLRYRFEIENTGDAR
                     PDMFIDVTFTPKQTAGTQVQTATIALSTGQTFTAPGTVPNLNPTPPDPVITTDAPTGI
                     RFFAGVTDDPFNFDITGFARYTAAQRACGPTFAADCTNAAKANLQRGRDSFAGYNILA
                     IVFSIPEALVRGQGNTVGANFLAQRRSNQTVAKRSQSTGNRVTGFGRWVTVDRMATPA
                     VNVALIPFNRKDEFNSATTIDDANGRFAASIVATLQALGTDQTSIGILAQIAVARGDF
                     VRVDLTIPNTNVGQPDANGNRDAAGFPNGRRFGDDVVDTELFLINNRVPLSDNANLPD
                     IPYRTVFPYIAPPHQPFPAGTIDDRTRN"
     gene            3480..5582
                     /locus_tag="AC159P_03085"
     CDS             3480..5582
                     /locus_tag="AC159P_03085"
                     /inference="COORDINATES: protein motif:HMM:NF027787.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007154 - cell communication [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Calx-beta domain-containing protein"
                     /protein_id="HIR3694468.1"
                     /translation="MNRARRVSRRRIAVALALAGLLSCLALPHRARVAGAPAASGGDD
                     GPRAASSVNASSGRLVFASERDDNYEIYIMDADGSSQFRLTSNTATDREPSISRDGAR
                     VAFTSNRDNNPEIYVMNADGSGQTRLTNTLADEGAPAFSPDGSQIAFVSNRNGNDEIF
                     VMNADGTGVANLTNNAADDNDPEWSPDGSAIAFTTDRDGNEEVYRMTSTGGAQVNLSN
                     NAADDAHPAWTPIKIYFQSDRGENFDIYSMNADGTAQTRLTTDPAFDLEPSGGTGSAR
                     VAFTSARDGSFELYFMNADGAAQTRLTNADGSNDLEPSVQTVLAPPAPQASTVQFSVS
                     TQSVSEGAGAATVTVTRTGSTTGASTVDFSATGGTASEVNDFTPAFGTLRFAAGETSK
                     TFNVLITDNAIAEFDETVNLTLSNATGAGFGALTTTTLTIVDNDAFTAATNPIDGAEF
                     FVRQHYADFLNRAPDAAGLAFWANQITACGNDAACIERARVQVSTAFFVSIEFQETGF
                     YVIRTQRAAFGRKSDTAASRIRLTQFLMDSQDVGKDVQVGVGAWQAQLDANKNAYAEQ
                     TATSADFIARYPLSLTAAEYVDALFATAMVTPTAAERQAAITAFGAGGTAGRAAALRA
                     VADSNSVRGAEFRPAFVLMQYFGYLRRNPTDPPDNNDAGYQFWLAKLNQFNGDPVAAE
                     MVKAFITSIEYRARFGPT"
     gene            complement(5746..>7730)
                     /locus_tag="AC159P_03090"
     CDS             complement(5746..>7730)
                     /locus_tag="AC159P_03090"
                     /inference="COORDINATES: protein motif:HMM:NF015763.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015099 - nickel cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035444 - nickel cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nickel/cobalt transporter"
                     /protein_id="HIR3694469.1"
                     /translation="GGAARALRCEDTNHGGRVGWHEIEVRPGPGVAVFDSSAYGSGAT
                     DELNRYPENTSGAALNERAAALSFTRGAAPAGSAPLLDRDGQAVKTAARDRFAELIEL
                     PELTPGVALLALLAAAALGGLHALSPGHGKAVVGAYLVGSRGTARHAAFLGLTVTITH
                     TSSVFALGLVTLFASQYVLPEQILPVLSLVSGAIVLALGLGLFVRRLRAALGASGHHH
                     HGHDHSHPHPPAHPHDQEHDHAHAHQHDHTHAHSHEHEHEHDGAHRHEHGHVHSHRGH
                     AGEAAHVHSHGGRAHSHLPPGADDAPVTWRSLLALGVSGGLLPCPSALVVMLAAIATG
                     RVAFGLLLIVAFSLGLAAVLTGIGLIFVYAGRWMKKTALGAGRLARVLPVVSAFVIAC
                     VGLALCYEALVQAGVDFGALVAGSQSPTTGASVLAFGLVLGLKHAVEADHLAAVTTIV
                     SERKSVLSASLVGGLWGLGHTVSLLVAGVAVILLRVEIGKRTEMALEFCVALMLVALG
                     ANAIRKLFRGGRLHLHAHQHGGHWHAHPHVHENGDEESARGAEEETHHGLRLGGRPFL
                     VGMVHGLAGSAAVMLLFLSAIHSPLMGLVYVAVFGLGSIGGMMLRSALVGLPVHFTAG
                     RFTRANLAIRCLAGLFSLGFGLFMVYEIGFVDGLFL"
BASE COUNT         1281 a         2882 c         2490 g         1077 t
ORIGIN      
        1 gagcacgtcg gccccgtccc cggtcaggag tggctgcact ccgtgacgct caggttcgac
       61 gacacgatgg gcgatgtcgg cgacgtgctg gtgcggctga tttacaaagg cgtggcgagt
      121 aaccgcgtgc gcgtcggcgt cggccacgtc ggcggcgggc cgccggacga cgcgggcgcc
      181 gtgccgacgc cggtgctcgc gctcgccatg ccgacgccca acgcgaaccc cgtgacggcg
      241 ggcaacctga cgacggacga cgtgcggcag gtcatcgcgc aggccgtctc ggcggcggtc
      301 gcgatgaacc gccgcgtgac ggtcgccgtc accgaccgcg agggcaacgt cctcggcgtc
      361 ttctctatga cgggcgcgcc cacccaaacg cagttccgcg gcgcaaccgg caacgacatg
      421 acgccgaacc cgatcaccgg cctcgtcccg cggggcttgg cggggacgaa cctgccgccc
      481 gtgttgattc cggtgccgcg cgccgccatc tcgaaggcgg gcaccgcctc cttcttcagc
      541 accttcggca acgccttcac gccgcgcacc gcctccttca tcatccagga acacttcccg
      601 cccagcgtca acttccagcc cggcggcccg ctctacggcg tgcagttctc gcaactgccg
      661 tgcagcgaca tcaagacgcc gacgctcccc ttcggcctgt cgggcgaccc cggcgccgtg
      721 ccgctctaca agaacggcgt gccctcgggc ggcgtcggca tcgagggcga cggcgtctat
      781 acgatcgacc gcgacgtgac ggatttcgat aagccgttcg aggaggtgat cgccgtcgcg
      841 gcggggcgcg gctacgagcc gcccggcctg attcgcggcg acaacatcat cgtcggcggc
      901 gtgcggctgc cctacgtcaa cgtgacggac gaagacgcgc cccgccccgc gaccatcgcc
      961 ttcggctccc tgcccggaca gatcgacccg atgttcccca tccgcggcgc gcagccctcg
     1021 gccttcgtgg cgaccaacgt gggcggcgtc aacggcgcgg cggacacgcg tttcttcccg
     1081 ttcatcagca gcccgagcgc ttcgtcgaac cggctgacgg ctgcggacgt ttcgaccatc
     1141 atcggcaacg ccgcgcggca ggccgacatc acgcgcgccg cgatccgcca gccgctgggc
     1201 agtcaggcgc gcgtctcgat cacggtggtg gacatcgagg ggcgcgtgct cggcatcttc
     1261 cgcacgaccg acgcgcccgt cttcggcttc gacgtgtcgg cgcagaaggc tcgctcctcg
     1321 gtcttcatct cgcgcccgga cgcgggcacg ctgctgcgcg ccgtcgggat gggtgcttac
     1381 gttgaccgcg cggcggcgga cggcctcggc ctgaacggcg cgattgcctt cagcgaccgc
     1441 gccttcggct tcctccaccg gcccttcttc cccgacggcc tgagcggcac gcccgccggc
     1501 cccttcagca ccgagatcgg cgagtggagc gtcttcaacc tcgggctgca aacggacgcc
     1561 atcacgacca acctcgtcgc cgcgctcgtc ggggccaacg tccgctgcac ggcgctgccg
     1621 caggtgccga acggcttcca gattttcgcg ggcggcatcc cgctctacaa gaacggcgag
     1681 ctggtgggcg ccatcggcat cagcggcgac ggcatcgacc aggacgacat catcgccgcc
     1741 gcgggggcca acggctacgc cccgcccgcc gccatgcgct ccgaccagac catcgtgcgc
     1801 gacacgcgtc tgcccttcgt caaattcccg cgcagcccga acctgtgaga tttagagggc
     1861 gcggcgcgaa tccgccgccg cggcggcgga cgtagtacct tcgacgctgt ttcaggaatc
     1921 ccgcacccgc ggcccgccgc cgcgggtcgc cggggcactc gatttcaggg gtgggtctcc
     1981 atgcgcgttt ccgtcaagcc ggctttcgag gtgaccgcgc cgcccgtccg cccgtccgcc
     2041 gccttcccgt cccgtcgtac acccgcgagg agataagaag atcatgcttc gtccgaacaa
     2101 caagagtgtc gccctcgtcg cgctggcgct gaccgccgcc ctcgcgctcc tgctcacgcc
     2161 cgcgccgcgc ggcagtgccg ccgaccacgg cgacgcgccg ttcgtcgcca acgaccgcgg
     2221 cgccgacatc gccgacacct acatcttccg cgacccggtg gacaactcgc gcgtcgtcat
     2281 cgccggcacc atcaacggct tcatcgtccc gaacgagatg gtcaacttcg gcgtcttcga
     2341 cgagaacctg cgctaccgct tcgagatcga gaacaccggc gacgcccgcc ccgacatgtt
     2401 catcgacgtg accttcacgc cgaagcagac cgccggcacg caggtgcaga ccgccaccat
     2461 cgcgctctcc accggccaga ccttcaccgc gcccggcacc gtcccgaacc tcaacccgac
     2521 gccgcccgac cccgtcatca cgaccgacgc gccgaccggc atccgcttct tcgcgggcgt
     2581 caccgacgac cccttcaact tcgacatcac gggcttcgcg cgctacacgg cggcccagcg
     2641 ggcctgcggc ccgaccttcg ccgccgactg caccaacgcc gccaaggcca acctccagcg
     2701 cgggcgcgac agcttcgccg gctacaacat cctcgccatc gtcttcagca tccccgaggc
     2761 gctcgtccgc gggcagggca acacggtcgg cgccaacttc ctcgcgcagc gccgcagcaa
     2821 ccagaccgtg gcgaaacgca gccagagtac cggcaaccgc gtgaccggct tcgggcgctg
     2881 ggtgaccgtt gaccggatgg cgaccccggc ggtcaacgtc gcgctcatcc cgttcaaccg
     2941 caaggacgag ttcaactcgg cgaccaccat cgacgacgcc aacggtcgct tcgccgcgag
     3001 catcgtcgcc acgctccagg cgctcggcac agaccagacc agcatcggca tcctcgcgca
     3061 gatcgcggtc gcgcgcggcg acttcgtgcg cgtcgatctg accatcccga acacgaacgt
     3121 cggccagccc gacgccaacg gcaaccgcga cgccgcgggc ttccccaacg ggcgccgctt
     3181 cggcgacgac gtggtcgaca ccgagctgtt cctcatcaac aaccgcgtgc ctttgagcga
     3241 caacgccaac ctgcccgaca taccgtaccg cacggtgttc ccctacatcg cgccgccgca
     3301 ccagcccttc ccggcgggca cgattgacga ccgcacgcgc aactgacgat tttagatttc
     3361 ggattgcgga ttggcgtgct gctttaactc gaagccggat ttcggacgtt caactgccgt
     3421 tgcctgaaca atccgcaatc cgcaatccac aatccgaaat cctctcaaga ggagccgtta
     3481 tgaatcgcgc acgtagagtc tcccggcgtc gcatcgcagt ggcgctcgcc ctcgccgggc
     3541 tgttgagttg tctggccctg ccgcaccgcg cccgcgtggc gggcgcgccc gccgcgtcgg
     3601 gcggcgacga cgggccgcgc gcggcgtcga gcgtcaacgc ctcttcggga cgcctcgtct
     3661 tcgccagcga gcgcgacgac aactacgaaa tctacatcat ggacgcggac ggaagctccc
     3721 agttccgcct gacctcgaac acggcaaccg accgcgagcc ttccatctcg cgcgacggtg
     3781 cgcgcgtcgc cttcacgagc aaccgcgaca acaaccccga aatctacgtg atgaacgccg
     3841 acggctccgg ccagacgcgg ctcaccaaca cgctcgcgga cgaaggcgcg cccgccttct
     3901 cgcccgacgg ctcgcagatc gccttcgtca gcaaccgcaa cggcaacgac gagattttcg
     3961 tcatgaacgc cgacggcacc ggcgtcgcga acctgacgaa caatgcggcg gacgacaacg
     4021 accccgagtg gtcgcccgac ggctcggcca tcgccttcac caccgaccgc gacggcaacg
     4081 aggaggtcta ccgcatgacc tcgaccggcg gcgcgcaggt caacctctcg aacaacgcgg
     4141 cggacgacgc gcaccccgca tggacgccga tcaaaatcta cttccagagc gaccgcggcg
     4201 agaacttcga catctactcg atgaacgccg acggcaccgc gcagacgcgc ctcacgaccg
     4261 atccggcctt cgacctcgaa ccctccggcg gaaccggcag cgcgcgcgtc gccttcacca
     4321 gcgcgcgcga cggcagcttc gaactctact tcatgaacgc cgacggcgcc gcgcagacgc
     4381 ggctcaccaa cgccgacggc agcaacgacc ttgagccgtc cgtgcagacg gtcctcgcgc
     4441 cgcccgcccc gcaggcgagc accgtgcagt tctccgtctc gacccagagc gtctcggaag
     4501 gcgcgggcgc ggccaccgtc acggtgacgc gcacgggcag cacgacgggc gcgtccaccg
     4561 tcgatttcag cgcgaccggc ggcacggctt ccgaggtgaa cgacttcacg cccgccttcg
     4621 gcacgctccg cttcgccgcg ggcgagacca gtaagacctt caacgtcctg attaccgata
     4681 acgccatcgc cgaattcgac gagaccgtca acctcacgct cagcaacgcg acgggcgcgg
     4741 gcttcggcgc gttgacgacg accacgctga ccatcgtcga taacgacgcc ttcacagccg
     4801 cgacgaaccc gattgacggc gcagagttct tcgtccgcca gcactacgcg gacttcctca
     4861 accgcgcgcc ggacgccgcg gggctagcct tctgggcgaa ccagatcacc gcgtgcggca
     4921 acgacgccgc gtgcatcgag cgtgcgcgcg tgcaggtctc gacggccttc ttcgtctcca
     4981 tcgagttcca ggagacgggc ttctacgtca tccgcacgca gcgcgcggcc ttcggcagga
     5041 agtccgacac cgccgcgtcg cgcatccgtc tgacgcagtt cctcatggac tcgcaggacg
     5101 tggggaagga cgtgcaggtc ggcgtcggcg cgtggcaggc gcaactcgac gccaacaaga
     5161 acgcctacgc cgagcagacg gcgacgagcg ccgacttcat cgcgcgctac ccgctctccc
     5221 tgaccgccgc cgaatacgtg gacgcgctct tcgccaccgc gatggtcacg cccaccgccg
     5281 ccgagcgcca ggccgccatc acggccttcg gcgcgggcgg caccgcgggc cgcgcggcgg
     5341 ctttgcgcgc ggtcgccgac tcgaactcgg tccgcggcgc ggagttccgc ccggccttcg
     5401 tgctgatgca gtacttcggc tacctcaggc gcaacccgac cgacccgccc gacaacaacg
     5461 acgccggcta ccagttctgg ctcgccaagc tcaaccagtt caacggcgac cccgtcgccg
     5521 ccgagatggt caaagccttc atcacctcca tcgaataccg cgcccgcttc gggccaacgt
     5581 aagaatagta ggcagtaggc agtaggcagt aggcagcaaa agcaagaggc ttgagatgta
     5641 aggtctcaag cctcttgctt cattgcatga agagcggaga ggcttggcac tttacctccc
     5701 aagcttcttc gctcttctgt cttgactgcc taccgcctac tgctcttaaa gaaagaggcc
     5761 gtcaacgaag ccgatttcgt agaccatgaa gaggccgaag ccgaggctga agagaccggc
     5821 gaggcagcgg atggcgaggt tggcgcgggt gaagcgcccg gcggtgaagt ggacgggcag
     5881 gccgaccagc gcgctcctca gcatcatgcc gccgatggag ccgaggccga agacggcgac
     5941 gtagacgaga cccatcagcg gcgagtggat ggcggagagg aagagcagca tcaccgccgc
     6001 gctccccgcg aggccgtgca ccatgccgac gaggaacggg cgcccgccga gacgcaggcc
     6061 gtggtgcgtc tcctcttcgg cgccgcgcgc gctctcttcg tcgccgtttt cgtgcacgtg
     6121 cgggtgcgcg tgccagtggc cgccgtgttg gtgcgcgtgc aggtggagcc gcccgccgcg
     6181 gaagagtttg cggatggcgt tcgcgccgag cgcgacgagc atcagcgcga cgcagaattc
     6241 gagcgccatt tcggtgcgtt tgccgatctc gacgcggagc aggatcacgg cgacgcccgc
     6301 gacgagcagc gagaccgtgt gcccgaggcc ccacagcccg ccgacgagcg aggcgctgag
     6361 cacgctcttg cgctcgctga cgatggtcgt cacggcggcg aggtggtcgg cctcgaccgc
     6421 gtgcttcaag ccgagcacca gcccgaacgc gaggacggac gcgccggtcg tcgggctttg
     6481 actgcccgcg acgagcgcgc cgaaatcgac gcccgcctgc accagcgcct cgtagcagag
     6541 ggccaggccg acgcacgcga tgacgaacgc gctcacgacc ggcagcacgc gcgcgaggcg
     6601 acccgccccg agggccgtct tcttcatcca gcggcccgcg tagacgaaga tcagcccgat
     6661 acccgtcagc acggcggcga gtccgagact gaacgcgacg atgagcagca ggccgaaggc
     6721 cacgcgcccg gtagcgatgg ccgcgagcat cacgacgagc gccgaagggc agggcagcag
     6781 cccgcccgaa acgccgagcg ccagcaggct ccgccacgtc accggcgcgt cgtccgcgcc
     6841 cggcggcaga tgcgaatgcg cccgcccgcc gtgcgaatga acgtgcgccg cctcgcccgc
     6901 gtgccctcga tgagaatgaa cgtgcccgtg ctcgtgtcga tgggcgccgt cgtgctcgtg
     6961 ctcatgctcg tgcgagtgcg cgtgtgtgtg gtcgtgctga tgggcatgag cgtggtcgtg
     7021 ctcttggtcg tgcggatgcg cgggcgggtg cgggtgcgaa tggtcgtgtc cgtggtgatg
     7081 gtgtcccgag gcgccgagag cggcgcgcag gcggcggacg aagaggccga ggccgagggc
     7141 gaggacgatg gcgccggaga cgaggctgag gacggggaga atctgttcgg gcagcacgta
     7201 ctgcgaggcg aagagcgtga cgaggccgag cgcgaagacg ctgctggtgt gcgtgatcgt
     7261 caccgtcagc ccgaggaagg ccgcgtgccg cgccgtgccg cgcgagccga cgagataggc
     7321 gcccacgacg gccttgccgt gccccggcga gagcgcgtgc agcccgccga gcgcggcggc
     7381 ggcaagcagc gccagcagcg cgacgcccgg cgtcagctcc ggcagctcga tcagctccgc
     7441 gaagcgatca cgcgctgccg tcttcaccgc ctgcccgtcg cggtcgagca gcggggcgga
     7501 acccgcgggc gccgcgccgc gcgtgaaaga cagcgccgcg gcgcgctcgt tgagcgcggc
     7561 ccccgacgtg ttctccgggt agcggttaag ctcgtccgtc gcgccgctgc cgtaggcgct
     7621 gctgtcgaag accgcgacgc ccggccccgg cctcacctcg atctcgtgcc agccgacgcg
     7681 cccgccgtgg ttcgtgtctt cgcaacgcag cgcgcgcgcc gcgccgccct
//
LOCUS       DBNGOH010000168         4269 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4424051, whole genome shotgun sequence.
ACCESSION   DBNGOH010000168 DBNGOH010000000
VERSION     DBNGOH010000168.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4269)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4269)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4269
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4424051"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1154
                     /locus_tag="AC159P_03095"
     CDS             <1..1154
                     /locus_tag="AC159P_03095"
                     /inference="COORDINATES: protein motif:HMM:NF024833.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YMGG-like glycine zipper-containing protein"
                     /protein_id="HIR3694470.1"
                     /translation="DLGELATAYNVAWNWDSTNTFPPPGGGYTASNRLTGTYRLDPSR
                     SDNARDIAERAARNLPAGERQRISERLASRLESPDMLAIERTGSRVTIASSRAPQTTF
                     DADGRVSNEQLPNGRASRVTATLSGERLTVRSTGFRENDFTVTFEPTGNGRQLQVTRE
                     IWNDRLGNTPIVVRNIYDRTSDVADFGVYNGSTASTYPPTTGGASGDYAIRDGEMIVA
                     AMNTDLTSRTTQTGDRFTMTVREPSQYRGAVIEGRVANVSRSGRITGRSEMALDFENI
                     RTPDGRTYRFAGFIETVRTPNGETVQVDNEGTVRDDDSRGTTTAQRAAIGTAVGAIIG
                     AIAGGGKGAAIGAIVGAAGGAGSVYVQGREDIELLSGTELTIRASAPNR"
     gene            1695..2843
                     /gene="hisZ"
                     /locus_tag="AC159P_03100"
     CDS             1695..2843
                     /gene="hisZ"
                     /locus_tag="AC159P_03100"
                     /inference="COORDINATES: protein motif:HMM:TIGR00443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HIR3694471.1"
                     /translation="MDSLSKIPNGMRYYFGAEARLRRAVEDEVMSVFDGWGYEEIAAP
                     TVDYYALFERGMGRGEAHRAFRFTDTDGRMLALRPDVTSSVARAAATLFASRARPLRF
                     CYAASVFRQQPRSRAEWRRQSRQVGCELIGVARLGAEMEVLLIAAEVLDRLGLRADAR
                     VTLSHAGLFNGVAAALGLEDEARERMRQIIDTRDAAELERFLSARAPTSGALELATRL
                     ARLSGKREALAEAKRAASSMNDDAAACKGVRAALGALEKLWDMIAGLGLADNFDIDLA
                     DVSGLDYYPGLVFRVYVAGAGARVGSGGRYDDLAANFGASEPAIGFVLDLDALAEVLA
                     RREAFRRRNGEQPHAVVEGAAETETFREALRRRAAGERVRLGAGDEEC"
     gene            2867..3469
                     /gene="hisG"
                     /locus_tag="AC159P_03105"
     CDS             2867..3469
                     /gene="hisG"
                     /locus_tag="AC159P_03105"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009793053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HIR3694472.1"
                     /translation="MQEDALKLLARAGVRLSGDDLASRRLAFADESGRYRFIFVKPAD
                     VPVYVEHGIADCGMVGRDVLLESEADLLQPLDLGIARCRLAVAAPRGARTRESGLLHV
                     ATKYPRTSAAHFGAKGVPVEIIELSGSVELAPVLGLADCIVDLVETGRTLEENGLAVV
                     ETITESTGRLAVNRASYQLKGEAVAELIDVLSKAVTEAKA"
     gene            3466..>4269
                     /gene="hisD"
                     /locus_tag="AC159P_03110"
     CDS             3466..>4269
                     /gene="hisD"
                     /locus_tag="AC159P_03110"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HIR3694473.1"
                     /translation="MIEIIKSEDARRRREKLARVKARSVAADADLMAVVAEIVEEVRR
                     MGDAALLDYTARFDGVRLTAAELRYDEAALAGAAGLAAREVVEALRASIRNVRAFHER
                     ERVSSWEMDAGRGVRLGQRVTPVEAAGLYVPGGTASYPSSVVMNVVPARVAGVGRVAV
                     VTPPGALRQAPAVAAALLELGVREVYQVGGAQAIAALAYGTETIPRVDVVTGPGNKYV
                     AAAKKLVFGAVGIDSIAGPSEVVVLADETARPDFVAADLLAQAEHGEDAS"
BASE COUNT          670 a         1484 c         1487 g          628 t
ORIGIN      
        1 cagacctcgg cgagctggcc acggcctaca acgtcgcttg gaactgggac tcgaccaaca
       61 cgttcccgcc tccgggtggc ggctacaccg cgagcaaccg cctgacgggc acgtaccggc
      121 tcgacccttc gcgcagcgac aacgcgcgcg acatcgcgga gcgcgccgcg cgcaacctgc
      181 ccgcgggcga gcgccagcgc atcagcgagc gtctcgcgag ccgtctcgaa tcgcccgaca
      241 tgctggccat cgagcgcaca ggctcgcgcg tcaccatcgc ctcttcgcgc gcgccgcaga
      301 ccaccttcga cgccgacggg cgcgtgagca acgaacagct cccgaacggc agggcttcgc
      361 gcgtcaccgc caccttgagc ggcgagcgcc tgaccgtccg ctcgacgggc ttccgcgaga
      421 acgatttcac cgtgaccttc gagccgacgg gcaacggtcg gcagcttcag gtcacgcgcg
      481 aaatctggaa cgaccggttg ggtaacacgc cgattgtcgt ccgcaacatc tacgaccgca
      541 cttccgacgt ggccgacttc ggcgtctaca acggctcgac ggcgagcacg tatccgccca
      601 cgacgggcgg cgcctccggc gactacgcca tccgcgacgg cgagatgatc gtcgccgcca
      661 tgaacacgga cctgacgagc cgcaccacgc agaccggcga ccgcttcacg atgaccgtgc
      721 gcgagccgtc gcagtatcgc ggcgccgtca tcgagggccg cgtcgccaac gtcagccgca
      781 gcggtcgcat cacgggccgc tccgagatgg cgctcgactt cgagaacatc cggacgcccg
      841 acggtcgcac ctaccgcttc gccggcttca tcgagaccgt gcgcacgccc aacggcgaga
      901 ccgtccaggt cgataacgaa ggcaccgtgc gcgacgacga cagccgcggc accacgaccg
      961 cgcagcgcgc cgccatcggc accgcggtcg gcgccatcat cggcgccatc gcgggcggcg
     1021 gcaagggcgc ggccatcggc gccatcgtcg gcgccgcggg cggcgccggc tccgtctacg
     1081 tccagggccg cgaggacatc gaactcctca gcggcaccga actgaccatc cgcgccagcg
     1141 cgccgaatcg gtaaaagcag taggcagcag gcagcgggca gtaggcagtg aaagccagag
     1201 gcttgagagc taacgtctca ggcctctggc ttttttgctt gaagcgcgga gaggctcagg
     1261 actttacttc tcaagcctct tcgcccttct gttttaactg cctgctgcct actgcccgct
     1321 gcctactgcc tacttttatt tgacatactt cgccgcactc gtttataaga accgtaaccc
     1381 gcgagccgcg gggagagctt caaagcgagt atgaccaacc acccgtccaa catcgcgctg
     1441 catcatggcg agcaccatcg ccacgcggcg tcgtgttgac ggcggtctcg gtcaggcgaa
     1501 cgcgcagctt aacagctcgc tgacgctccc agaatcgaac gaacgccgcc ggcacacgca
     1561 accggcggcg tttctctttt aacgatgaat gcggagtgat gaatgatgaa tcgaagacag
     1621 gctgaatggt gacagtcagc gtgcttcgta ttcatcattc cgcattcatc attccgcgtt
     1681 catcatttgt cccgatggat tctctctcga aaatcccgaa cggcatgcgc tactacttcg
     1741 gcgcggaggc gcgtttgcgc cgcgcggtcg aggacgaggt gatgtccgtg ttcgacgggt
     1801 ggggctacga agagatcgcc gcgccgaccg tggactacta cgcgctgttc gagcgcggca
     1861 tggggcgcgg cgaggcgcac cgcgcgttcc gcttcacgga cacggacggg cggatgctcg
     1921 cgctcaggcc cgacgtgacc tcttcggtgg cgcgcgccgc ggcgaccctc ttcgcctcgc
     1981 gcgcgcggcc cctgcgcttc tgctacgcgg cctccgtctt ccgccagcag ccgcgctcgc
     2041 gcgccgagtg gcgcaggcag agccgtcagg tgggctgcga gctgatcggc gtcgcgcggc
     2101 tcggcgcgga gatggaggtg ctgctcatcg ccgcggaggt gctcgaccgc ttgggcctgc
     2161 gcgccgacgc gcgcgtgacg ctgagccacg cggggctgtt caacggcgtc gccgccgcgc
     2221 tcggactcga agacgaagcg cgcgagcgga tgcggcagat tatcgacacg cgcgacgccg
     2281 ccgaactcga aagatttttg tcggcgcgcg cgcccacgtc cggcgcgttg gagctggcga
     2341 cccgcctcgc gcgcctctcg ggcaaacgcg aggcgctcgc ggaggcgaag cgcgcggcct
     2401 cctccatgaa cgacgacgcc gcggcgtgca agggcgtgcg cgcggcgctc ggcgcgctcg
     2461 aaaagttgtg ggacatgatc gcgggcctcg gcctcgcgga caacttcgac atcgatcttg
     2521 cggacgtgtc gggcttggac tactacccgg gcctcgtctt ccgcgtctac gtcgcgggcg
     2581 cgggcgcgcg cgtcggcagc ggcgggcgct acgacgatct ggccgccaac ttcggcgcta
     2641 gtgagccggc catcggcttc gtgctcgacc tcgacgcgct cgccgaggtt ctcgcgcggc
     2701 gcgaagcatt ccgccgccgc aacggcgagc agccgcacgc ggttgtcgaa ggcgcagccg
     2761 agaccgagac gttccgcgaa gccctgcgca ggcgcgcggc gggcgagcgc gtgcggctcg
     2821 gcgcggggga cgaagaatgc tgaccgtcgc cgtcgcaaag ggaaggatgc aggaagatgc
     2881 gctcaagctt ctcgcgcgcg cgggggttcg cttgagcggc gacgacctcg cctcgcgccg
     2941 tctcgcgttc gcggacgagt cggggcgcta ccgcttcatc ttcgtcaagc ccgcggacgt
     3001 gcccgtctac gtcgagcacg gcatcgcgga ctgcggcatg gtgggccgcg acgtgctgct
     3061 cgaatcggag gcagacctct tgcagccgct cgatttgggg atcgcgcgct gccgcctcgc
     3121 cgtcgccgcc ccgcgcggcg cgcgcacgcg cgagtcgggg ctgctccacg tggcgacgaa
     3181 gtacccgcgc acctccgccg cgcacttcgg cgcgaagggc gtccccgtcg agatcatcga
     3241 gctttccggc tcggtcgagc tggcgcccgt gctcggcctc gccgactgca tcgtcgatct
     3301 ggtcgagacc gggcgcacgc tcgaagagaa cggcctcgcc gtcgtcgaga cgatcaccga
     3361 atcgaccggg cgcctcgccg tcaaccgcgc gagctaccaa ctgaagggcg aggccgtcgc
     3421 ggaactcatc gacgtgttga gcaaggccgt gacagaggca aaggcgtgat cgagataatc
     3481 aaaagtgaag acgcgcggcg gcgacgcgaa aagctcgcgc gcgtgaaggc gcggagcgtc
     3541 gcggcggacg cggatttgat ggcggtcgtc gcggagatcg tcgaagaggt gcggcggatg
     3601 ggcgacgcgg cgctgctgga ttatacggcg cgcttcgacg gcgtgcggct cacggccgcc
     3661 gagctgcgct atgacgaggc ggcgctcgcc ggggcggcgg ggctcgccgc ccgcgaagtg
     3721 gtcgaggccc tgcgcgcgag catccgcaac gtccgggcgt tccacgagcg cgagcgcgtc
     3781 agttcgtggg agatggatgc cgggcgcggc gtgcgactcg ggcagcgcgt cacgccggtc
     3841 gaggcggccg ggctctacgt gccgggcggg acggcgagct atccttcgtc ggtggtgatg
     3901 aatgtcgtgc cggcgcgcgt ggccggggtc gggcgcgtcg cggtcgtgac gccccccggc
     3961 gcgctgcggc aggcccccgc cgtcgccgcc gcgctcctcg aactcggcgt cagagaggtc
     4021 taccaggtgg gcggcgcgca ggccatcgcc gcgctcgcct acgggacgga gacgatcccg
     4081 cgcgttgacg tcgtgacggg gccgggcaac aagtacgtcg cggcggcgaa gaagctggtc
     4141 ttcggcgccg tcgggattga ttccatcgcc gggccgagcg aggtcgtcgt cctcgccgac
     4201 gagaccgccc ggcccgactt cgtcgcggcc gatctgctgg cgcaggccga gcacggcgag
     4261 gacgcctcg
//
LOCUS       DBNGOH010000169         3188 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_941511, whole genome shotgun sequence.
ACCESSION   DBNGOH010000169 DBNGOH010000000
VERSION     DBNGOH010000169.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3188)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3188)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3188
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_941511"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="AC159P_03115"
     CDS             complement(<1..222)
                     /locus_tag="AC159P_03115"
                     /inference="COORDINATES: protein motif:HMM:NF012286.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate/malate family dehydrogenase"
                     /protein_id="HIR3694474.1"
                     /translation="MRNTNHNERLDRIAVVGCGHVGATAAYSLLLGGVANEIVLIDAN
                     PRCAEGEAMDLQHAVPLARPARANTTTPTS"
     gene            complement(479..2008)
                     /locus_tag="AC159P_03120"
     CDS             complement(479..2008)
                     /locus_tag="AC159P_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming cytochrome c nitrite reductase
                     subunit c552"
                     /protein_id="HIR3694475.1"
                     /translation="MAENEKEEAREEAAAAPSKRGRRSVRIIVLAALVAALAAVAGVA
                     LLVNIMERKQEARNPFYRVVELTDETVDPEVWGKNFPLQYDGYRRTVDQVRTKYGGSE
                     AEPRTPTQADPRSVVAQSRLEEDPRLKAIWAGYAFSKDFREERGHAYMLTDQIYTERQ
                     QAAQQPGTCIHCHASVYVPYKQAGGGDLIKGFEHFNRMPFAEAKKSFTHPVSCIDCHD
                     SQTMQLRVTRPGFIEGVRALAASEDPAPHLPSVERWRAGGRKGTYDVNAQATRQEMRA
                     FVCGQCHVEYYFKGPEKRLTYPWHKGLKVEQIMAYYDEVKHNDWQHAESGAGVLKAQH
                     PEFEMWNQGIHARSGVSCADCHMPYVREGALKISDHHVRSPMLNINNACQVCHKWPEE
                     ELRLRVETIQGRTHRMRDLAMDALVELINDIKAARTAGRTDAELQTARDFQRKAQFYL
                     DFVEAENSTGFHAGQEAVRILGESINFSRQGQVALRGGVQPARANTTTPTSFMRTSLK
                     R"
     gene            complement(2074..2541)
                     /gene="nrfH"
                     /locus_tag="AC159P_03125"
     CDS             complement(2074..2541)
                     /gene="nrfH"
                     /locus_tag="AC159P_03125"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase small subunit"
                     /protein_id="HIR3694476.1"
                     /translation="MNSQTIKGIVLGVAFGAAIGIGGFTFIYAKGYSYLTNDPAACAN
                     CHVMEAQYSGWLKSSHRSVAVCNDCHTPPGTLGKYYTKASNGFWHSYYFTTDTFHEPI
                     RITGRNARVTEQACRKCHEPVTSAIDAHMGGGEAGGEGALSCVRCHRNVGHMH"
     gene            complement(2688..>3188)
                     /locus_tag="AC159P_03130"
     CDS             complement(2688..>3188)
                     /locus_tag="AC159P_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020464771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIR3694477.1"
                     /translation="VHRWPLVEAAEQLGQRDWAESYRTVGQFMSTDLFTVRPDDLVDL
                     AASVMDWRHIRHVPVEDEAGRLVGLVTHRDILRLLVRGLQPARDGASVLVREIMKAAP
                     VSVGPATPTLEAVELMRTHGVGCLPVVEDGALVGIVTAKDFLDASARLFAERLAPPPE
                     GRSAGV"
BASE COUNT          457 a         1043 c         1115 g          573 t
ORIGIN      
        1 cgacgttggg gtcgtcgtgt tcgcgcgcgc gggccgcgcg agcggcacgg cgtgctgcaa
       61 gtccatcgcc tcgccctcgg cgcaccgcgg gttcgcgtcg atgagcacga tctcgttggc
      121 gacgccgccc agcagcaggc tgtacgcggc ggtcgcgccg acgtgaccgc acccgacgac
      181 ggcgatgcgg tcgagccgtt cgttatggtt cgtgtttctc atcgttccgg gccgagggtt
      241 tcggccagca tgcgcggcga taatagaccg ccggggaaaa gggatggaag acggttggct
      301 gcgcgcggct gcggcgtcgc ggttgagtgt ggcgcgggtt catttcaaca cggaggacac
      361 ggaggattca cagagggcac agagaagaca agttgctttc tctgtgccct ctgtgaatcc
      421 tccgtgtcct ctgtgttgaa atactgcccc ggtagattcg acgacagttg aagcgtgtct
      481 atcttttcaa agacgtgcgc atgaacgacg ttggggtcgt cgtgttcgcg cgcgcgggct
      541 ggacgccgcc gcgcagggcc acctgcccct gccgcgagaa gttgatcgac tcgcctaaaa
      601 tccttaccgc ctcctgcccg gcgtggaagc ccgtcgagtt ctcggcttcg acgaagtcga
      661 ggtagaactg cgccttgcgc tggaagtcgc gggccgtctg tagctcggcg tcggtgcgcc
      721 cggcggtgcg cgccgccttg atgtcgttga taagctcgac gagagcgtcc atcgcgaggt
      781 cgcgcatgcg gtgggtgcgc ccttgaatcg tctcgacgcg caggcgcagc tcctcctcgg
      841 gccacttgtg gcagacctgg caggcgttgt tgatgttgag catcgggctg cggacgtggt
      901 ggtcgctgat cttcagagcg ccctcgcgca cgtagggcat gtggcagtcg gcgcacgaga
      961 cgcccgagcg cgcgtggatg ccctggttcc acatctcgaa ctcggggtgc tgcgccttca
     1021 gcacgccggc gcccgactcg gcgtgctgcc agtcgttgtg cttcacctcg tcgtagtagg
     1081 ccatgatctg ctcgaccttc agccccttgt gccacggata agtcaggcgc ttctccggcc
     1141 ccttgaaata gtattcgacg tggcactggc cgcagacgaa ggcgcgcatc tcctgacggg
     1201 tcgcctgcgc gttcacgtcg tacgtgccct tgcgcccgcc tgcgcgccag cgctcgacgc
     1261 tcggcagatg cggcgccggg tcttcggacg cggcgagcgc gcggacgccc tcgatgaagc
     1321 cggggcgcgt gacgcgcagt tgcatcgtct gcgagtcgtg gcagtcgatg caggagacgg
     1381 ggtgtgtgaa ggacttcttc gcctcggcga agggcatccg gttgaagtgc tcgaagccct
     1441 tgatgaggtc gccgccgccc gcctgtttgt aaggcacgta gaccgaggcg tggcaatgaa
     1501 tgcacgtgcc gggctgctgc gcggcctgct ggcgctcggt gtaaatctgg tcggtgagca
     1561 tataggcgtg gccgcgctcc tcgcggaagt ccttcgagaa ggcgtaaccc gcccatatcg
     1621 ccttgaggcg cgggtcttct tcgaggcgcg actgcgcgac gaccgagcgc gggtcggcct
     1681 gcgtgggcgt gcgcggctcg gcctcgctcc cgccgtactt cgtccgcacc tgatcgaccg
     1741 tgcgccggta gccgtcgtac tgcaaaggga agttcttgcc ccacacctcc gggtcaaccg
     1801 tctcgtccgt caactcgacg acgcggtaga aggggttgcg cgcctcctgc ttgcgctcca
     1861 tgatgttgac gagcagcgcc acgcccgcca ccgccgcgag cgccgcgacg agcgccgcca
     1921 gcacgatgat cctgacgctc cgccgcccgc gcttcgacgg cgctgccgcg gcctcttccc
     1981 ttgcctcttc cttttcgttc tcagccattg cctctcacct cagactcgcc gggccgcggc
     2041 ccggccacgt acgccgcgcg cggcaggagt tgctcagtgc atgtgcccga cgttgcggtg
     2101 acagcggacg cacgagagcg ccccttcgcc gccggcctcg ccgccgccca tgtgcgcgtc
     2161 gatggcgctc gtcacaggct cgtggcactt gcggcacgcc tgctccgtga cgcgcgcgtt
     2221 gcgccccgtg atgcgtatcg gctcgtggaa ggtgtcggtc gtgaagtagt acgaatgcca
     2281 gaagccgttc gaggctttcg tgtagtactt gcccagcgtg ccgggcggcg tgtggcagtc
     2341 gttgcagacg gcgaccgagc ggtgcgaaga cttgagccaa ccgctgtatt gcgcctccat
     2401 gacgtggcag ttggcgcagg ccgccgggtc gttcgtgagg taggagtagc ctttggcgta
     2461 gatgaacgtg aagccgccga tgccgatggc cgcgccgaac gcgacgccga ggacgatccc
     2521 cttgatggtc tgcgagttca tcacgaaacc gacgcgcgcc cggcggactg cgcgcgggcg
     2581 aggataaagg gcggcactcc gccccaaact cgttatcgct tgtgggggtt gctgctacgg
     2641 cgtgacgaat atagcacgtg cggcgggcgc cgggggtgtg acgcgcgtca cacccccgcg
     2701 ctgcgccctt cgggcggcgg cgcgaggcgc tccgcgaaga gccgggccga ggcgtcgagg
     2761 aagtccttcg ccgtgacgat gccgacgagg gcgccgtcct cgacgacggg cagacagccg
     2821 accccgtgcg tgcgcatcag ctcgacggct tcgagcgtcg gcgtcgcggg gccgaccgag
     2881 acgggcgcgg ccttcatgat ctcgcggacg agcaccgacg cgccgtccct cgccggctgg
     2941 agaccgcgca ccagcaggcg caggatgtct cgatgcgtga cgagtccgac cagccgtccc
     3001 gcttcgtcct cgacggggac gtggcggatg tgccgccagt ccatgacgct cgcggccaag
     3061 tccacgaggt cgtccgggcg gacggtgaag aggtctgtgg acatgaactg cccgacggtg
     3121 cggtagcttt cggcccagtc gcgctgcccg agctgctctg cggcctcgac gagcggccaa
     3181 cggtggac
//
LOCUS       DBNGOH010000170         2561 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2774469, whole genome shotgun sequence.
ACCESSION   DBNGOH010000170 DBNGOH010000000
VERSION     DBNGOH010000170.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2561)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2561)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2561
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2774469"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1037)
                     /locus_tag="AC159P_03135"
     CDS             complement(<1..1037)
                     /locus_tag="AC159P_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012930170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S41"
                     /protein_id="HIR3694478.1"
                     /translation="MSKRERLVSRLCVVVLLLNAVVAPSNVRAQGARAALPDSRPEAR
                     PYFYEPSISPDRAEIAFVSGGDIWTVAAAGGEARLLVSHAATEYRPLYSPDGRRLAFT
                     SARTGNGDVYVLALDTGELRRLTFDDGFDQLDAWSRDGRWLYFTSTARDISASNDIYR
                     VGAEGGTPMQVSADRYANEFMSAPSPDGQDLLFVGKGYLQWWRNGHSHMDTSELWLMR
                     GHSTARYEKLTEGGAEEAWPMWGAGGRSVYYVSDRGGAENVWALAHGGGNNNRPRQVT
                     RFTEGRVMWPSISADGRVIVFERGFGIWSLDTESGQAREVNIRRRGASASPGVERVRQ
                     TDQFQDLALSP"
     gene            1282..2172
                     /locus_tag="AC159P_03140"
     CDS             1282..2172
                     /locus_tag="AC159P_03140"
                     /inference="COORDINATES: protein motif:HMM:NF013811.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694479.1"
                     /translation="MSVLLIFVDGLGIGTRGPHNPLHLLGDRAAPLAVFEGEEPELPH
                     GGVLVRTDATLGVEGRPQSASGQTTILTGVNAPARLGYHKQGFPNEGLRDIIREHSIF
                     LQLKRAGVGPNVFANTYTRRFFDARPRWVSATTVAVEAAGLPFRTVEDLRAGRAVFHD
                     FTNATIRERGEEADPRTPEEAAAVLASVAREHRFTLYEHFLTDRAGHEQDAEAALHIL
                     ANLGRFVRSVLSAVDLARTTVMLTSDHGNVEDLSARNHTLNLVPTLAWGPGREEVARG
                     VRSLADITPTIIRILTTARD"
     gene            2182..>2561
                     /locus_tag="AC159P_03145"
     CDS             2182..>2561
                     /locus_tag="AC159P_03145"
                     /inference="COORDINATES: protein motif:HMM:NF023128.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3694480.1"
                     /translation="MSEHIAPARAADPAADIPDSTTRKNDPREVFGWMTYDWANSAFY
                     TTVVGALYGEYLTYVTQQSVGAEGVALSLGPLGSISAKSYDPLCITLAVMLQIFLLPL
                     LGAIADYSNLKKRLMAVFCYTGASA"
BASE COUNT          417 a          985 c          756 g          403 t
ORIGIN      
        1 tcgggcgaga gcgcgaggtc ttggaactgg tctgtctggc gcacgcgctc gacgcccggc
       61 gacgccgacg cgccgcgacg acggatgttg acctcgcgcg cctgtccgct ctccgtgtcg
      121 agcgaccaga tgccgaagcc gcgctcgaag acgatcacgc gaccgtccgc cgagatgctc
      181 ggccacatca cgcgcccttc ggtgaaccgt gtcacctgcc gcggcctgtt attgttgccg
      241 ccgccgtgcg cgagcgccca cacgttctcc gcgccgcccc ggtcggagac gtagtagacg
      301 ctgcggcccc ccgcgcccca catcggccag gcctcctcgg cgccgccctc ggtcagcttc
      361 tcgtacctcg ccgtcgagtg gccgcgcatc agccacagct ccgaagtgtc catgtggctg
      421 tggccgttgc gccaccactg caagtagccc ttgccgacga agagcaagtc ctgcccgtcc
      481 ggcgacggcg ccgacatgaa ctcgttggcg tagcggtcgg cgctgacctg catcggcgtg
      541 ccgccctccg cgccgacgcg gtagatgtcg ttgctcgccg agatgtcgcg cgccgtcgag
      601 gtgaagtaga gccagcgccc gtcgcgcgac cacgcgtcga gctggtcgaa gccgtcgtcg
      661 aacgtgagcc gccgcagttc gcccgtgtcg agcgcgagca cgtacacgtc gccgttgccc
      721 gtccgcgccg aggtgaacgc gagccgccgc ccgtcgggcg agtagagcgg tcggtactcg
      781 gtcgccgcgt gcgacaccaa aagccgcgcc tcgccgcccg ccgccgcgac cgtccagatg
      841 tcgccgcccg agacgaacgc gatctccgcg cggtcgggcg aaatcgaagg ctcgtagaag
      901 taaggccgcg cttcgggccg tgagtcgggc agggccgccc tcgcgccttg cgccctcacg
      961 ttcgaggggg cgacgaccgc gttcagcaac aacacgacga cgcacagacg gctcacgagc
     1021 ctttcacgct tactcatcat ttcctccgta agatttctcg cgcgcgaaac cgcgacttga
     1081 ggttttcccc agaccgaagt tgcatcacta tagcagacac ccggcgaata acattccgcc
     1141 tcgcccggat ttcttcctca cccgtccgcg cgctcgcaag acatccattt gcttagacct
     1201 ctcgtagaaa taaccgcctg cggattccct gccggtgtcg gccttgcgtc gcgcgcacgt
     1261 tgaatgctac ttatcctccc gatgtccgtg cttctcatct tcgtggacgg cctcggcatc
     1321 ggcacgcgcg ggccgcacaa cccgctccac ctcttgggcg acagggccgc gccgctcgcc
     1381 gttttcgaag gcgaggagcc ggagcttccg cacggcggag tgctggtgcg cacggacgcg
     1441 acgctcggcg tcgagggccg accgcagagc gcctcgggcc agacgaccat cctcaccggc
     1501 gtcaacgcgc ccgcccgtct cggctaccac aagcagggct tccccaacga gggcctccgc
     1561 gacatcatca gagagcactc gatcttcctc cagctcaagc gcgccggggt cggccccaac
     1621 gtcttcgcca acacctacac gcgccgcttc ttcgacgcgc gcccgcgctg ggtctcggcc
     1681 acgacggtcg ccgtcgaggc cgcaggtctg cccttccgca ccgtcgaaga cctgcgcgcc
     1741 ggtcgcgccg tcttccacga cttcaccaac gccacgatcc gcgagcgcgg cgaagaagcc
     1801 gaccccagaa cgcccgaaga ggccgccgcc gtcctcgcct ccgtcgcgcg cgagcaccgc
     1861 ttcacgctct acgaacactt cctcacagac cgcgccggcc acgaacagga tgccgaagcc
     1921 gcgcttcaca tcctcgccaa cctcgggcgc ttcgtccgct ccgttctttc agccgttgac
     1981 ctcgcgcgca cgaccgtcat gctcacgagc gaccacggca acgtcgaaga cctctcggca
     2041 cgcaaccaca cgctcaacct cgtgcccacg ctcgcgtggg ggccggggcg cgaagaagtc
     2101 gcgcgcggcg tccgcagcct cgccgacatc accccgacga tcatccgcat cctgacgacc
     2161 gcaagagact aggccaacag catgagcgaa cacatcgccc ccgcacgcgc cgccgacccc
     2221 gccgcagaca ttcccgacag cacgactcgc aagaacgacc cgcgcgaggt cttcggctgg
     2281 atgacctacg actgggccaa ctcggccttc tacacgacgg ttgtcggcgc gctctacggc
     2341 gagtacctga cctacgtcac gcagcaatcg gtcggcgccg aaggcgtcgc gctctcactc
     2401 ggcccgctcg gctccatctc cgcgaaatct tacgacccgc tctgcatcac gctcgccgtg
     2461 atgctccaga tattcctgct gccgctcctc ggcgccatcg ccgactactc gaatttgaag
     2521 aagcgtttga tggccgtctt ctgctacacg ggcgcgagtg c
//
LOCUS       DBNGOH010000171         1560 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2224899, whole genome shotgun sequence.
ACCESSION   DBNGOH010000171 DBNGOH010000000
VERSION     DBNGOH010000171.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1560)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1560)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1560
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2224899"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            237..602
                     /locus_tag="AC159P_03150"
     CDS             237..602
                     /locus_tag="AC159P_03150"
                     /inference="COORDINATES: protein motif:HMM:NF012790.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HIR3694481.1"
                     /translation="MRTALLASTFSVIALLTLCACNAVPADNGNAGRGAPTESNRHAS
                     QQPSPDTSSDPFATVPRVSVEELSKALKEGRAVVVDVRPIEAFEAERIRGALSIPEEE
                     VKERAGELSPDKLVVTYCA"
     gene            complement(924..1358)
                     /locus_tag="AC159P_03155"
     CDS             complement(924..1358)
                     /locus_tag="AC159P_03155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694482.1"
                     /translation="MPHEAKRKRPVTTAFMILLVACLPLIAPQAQREGRLAPPPSLRC
                     PRDNTTSFTGRVLAYRRTPGRVFIRVRTDEETTEQFTLTHPKRGDAASLFLIDGGPFK
                     RSDWRRIEARAGRLRPNMRATVWACYEGDQPKAEVIDWRPPA"
BASE COUNT          296 a          513 c          497 g          254 t
ORIGIN      
        1 gccggcaacg accccatcga gaacttcatg gactacaccg acgacttctg catgtacaag
       61 ttcaccgccg gccagtccac gcgcgccgac gggatgtggg cgacctaccg cgcgggcaag
      121 taagcgccga acggacgcac cacacgcacg cttcacgaaa cagggagcgc tcacttcaag
      181 agcgctccct tttcatcgac gcgactcgca cgacggagca gaatacgacc tgaccgatga
      241 gaacagcgct gctcgcatcg accttctccg tcatcgctct gctaacgctg tgcgcctgca
      301 acgccgtgcc tgcggacaac ggtaacgcgg ggcgcggcgc cccaacggag tccaaccggc
      361 acgcctcgca acaaccttca cccgacacat cgtcagaccc tttcgccaca gtcccgcgcg
      421 tgtccgtcga ggaattatcg aaggctttga aggaaggccg cgccgtcgtc gtggacgtgc
      481 ggcccatcga agcgttcgag gcggagcgca tccgcggcgc gctctcgatt ccggaagagg
      541 aggtcaagga gcgcgcgggc gagttgtcgc ccgataagct cgtcgtcacc tactgtgctt
      601 gacccgccga acactccagc gcccgtgcgg cgtcctacct cagacacaaa ggcatcaagg
      661 agaccgcggc gctcgtcggc ggactcgacg catggaaggc ggcccaactc ccattggata
      721 aagggaagcc ccgccgttaa cgcgcgaccg gttggaaaac gagaggcagg attcgacgca
      781 gagaacgcag aggggcgcgg aggacacaga gaagaaaagg ttttctccgc gccctccgcg
      841 cccctctgcg ttctccgcgt ttaaaagggc gcgcggcaag ctcaaccgca cactgtattc
      901 gacagctcgc tcccacggag tttttaagct ggcggtcgcc agtcgatcac ttctgccttc
      961 ggctggtcgc cctcgtaaca ggcccacacc gtcgcacgca tgttgggccg gagccggccc
     1021 gcgcgcgcct cgatgcgccg ccagtcgctc cgcttgaagg gcccgccgtc gatgagaaag
     1081 aggctcgccg cgtcgccgcg cttcgggtgt gtgagcgtga actgctcggt cgtctcctcg
     1141 tccgtgcgga cgcgtataaa gacgcggccc ggtgtgcgcc ggtaggcgag cacgcggccc
     1201 gtaaacgagg tcgtgttgtc gcgcgggcac ctcaaagacg gcggaggggc gagccgcccc
     1261 tcgcgctgtg cctgcggcgc gatgagcggc aggcaggcga cgagtaagat catgaaggcc
     1321 gtcgtgaccg ggcgtttgcg cttcgcttcg tgcggcatcg tttcttgcac ctcacactgt
     1381 agatgacacc gcgaccggcg cgtctgcccg ccggctttgt tacatttgtg acacgggggc
     1441 cggaggccac tacaaaataa tcccggcaag cgataatatt cgcggagtgt gaaaatcgcg
     1501 acgtggaatg tgaattcggt gctggcgcgg ctgccgttgg tgacgcgctg gctcgcggcg
//
LOCUS       DBNGOH010000172         6911 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1125647, whole genome shotgun sequence.
ACCESSION   DBNGOH010000172 DBNGOH010000000
VERSION     DBNGOH010000172.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6911)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6911)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6911
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1125647"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..489)
                     /gene="ruvA"
                     /locus_tag="AC159P_03160"
     CDS             complement(<1..489)
                     /gene="ruvA"
                     /locus_tag="AC159P_03160"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HIR3694483.1"
                     /translation="MIAHLSGTLREKQATSVILDVGGVGYEVTIPVTTFFNMEEPGSQ
                     VQLRIYTHVREDTLQLFGFRTPRERELFTLLISVTGIGPKSGIAMLSGMSADEIVTAI
                     RTNNLARLTSIPGVGRKTAERLVIELRDKMAALSSPALDAEIEAGAGLAAAPSEDALR
                     EDT"
     gene            complement(749..1438)
                     /locus_tag="AC159P_03165"
     CDS             complement(749..1438)
                     /locus_tag="AC159P_03165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694484.1"
                     /translation="MSRPKFELSLLFVLFASVPVVASAQVEGNPENWCRNGLYTHDAA
                     GFRVGHVVGAKGARAYFFGDASEDCPGAGAKCRQRAYVLPGDAVVVSRRLGDYVCAWF
                     APARGPETVGWLRAEQVSLSEADAGPPLARWLGAWEYAGQSLDIRRGKKAGALAVSGQ
                     AFWRGQGDNVHVGEVEFEAAPAGNVLKLEENEDLCSVTIRLVGPHLVVSDNLKCGGLN
                     VTFNGIYRKKQ"
     gene            complement(1516..2463)
                     /locus_tag="AC159P_03170"
     CDS             complement(1516..2463)
                     /locus_tag="AC159P_03170"
                     /inference="COORDINATES: protein motif:HMM:NF018899.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HIR3694485.1"
                     /translation="MKRVLVLCAQLLLAVAPAAAQGANPATPEEKCDGPVYKMTEVSR
                     KPVIKHKPDPGFTEEARSNNASGTVALAAVLCHTGRVTDIRIMKSLPFGLTEISVAAA
                     SRVEFTPAEKDGRRVATHIRFEYNFNTFGGSALARLGGSDNNRFTALLSDKHNYRLVE
                     DLLIEGNRRLRDEEIYDKLRTRPGDRFNVEQVQRDLQALLDMAFFDKTETRVAVEEGP
                     RGGVCVIFYVKELPIIRDFTFKGLSSIGEADVLKALGESGVRVCKECPYDPVQIVRAK
                     QIIRQLLAARGWPDATIEVSVEEISQVSVTLAFIVNERP"
     gene            complement(2557..3717)
                     /locus_tag="AC159P_03175"
     CDS             complement(2557..3717)
                     /locus_tag="AC159P_03175"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020081591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DmpA family aminopeptidase"
                     /protein_id="HIR3694486.1"
                     /translation="MCVLLFAAHGARAQTKPRARDLGVPFEGTPGPLNAITDVKGVEV
                     GHTTLVSGEGKLVAGRGPVRTGVTAILPQGKTFRGRVFGAWHTLNGNGEMTGTTWLEE
                     SGALGAPVLITNTHSVGVVRDAVIEWNARRGTGEGYSGDFSLPVVAETWDGFLNDING
                     FHVKKEHAFQALDSARGGPVAEGNVGGGTGMIAHGFKGGIGTSSRMLGAEAGGYTLGV
                     LVQANYGSRQLLRIAGVPVGREITDLLPERGKPVAEGGGADEGAGSIIVVLATDAPLL
                     PHQLKRLVRRVALGVGVMGGRGENSSGDIFIAFSTANPEVGKTEGTAQLTMLPNERIN
                     PLLRAAADATEEAIVNAMVAAETMRGIDGNTAHALPHERLREALRKYNRLTK"
     gene            complement(3827..5362)
                     /locus_tag="AC159P_03180"
     CDS             complement(3827..5362)
                     /locus_tag="AC159P_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016984490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3694487.1"
                     /translation="MTQNSSAAAGAAARGGTGDYKIWQVIAASAVGTMIEWYDFYIFG
                     SLATTISPLFYPQGNDTLALIAYLSTFAVGFVVRPFGALFFGRIGDLVGRKYAFLVTL
                     LIMGGATACIGILPTYESIGIAAPLLLLLIRVLQGLALGGEYGGAAVYVAEHVPDGKR
                     GFYTSFIQITATLGLFVSLAVILLVQNALSREAFTAWGWRVPFLISILLVGVSLYIRL
                     KMKESPIFSHIKSAGMTSAQPLKDAFTKWSNLKLVLISLFGATAGQGVVWYAGQFYAL
                     FYLETILRVNPTSAKYIVAAALLLGMPLFVFFGALSDRIGRKWIIMVGCLLAAVSYIP
                     IYKAMQAAAGSNVVTAASRPDRVTGAATLTPLAEVNGALVPAPEVLPYADIGSLLTNA
                     TAWKLILLVFVQVVFVTMVYGPIAAFLVESFPAKIRYTALSLPYHIGNGIFGGLLPVI
                     GLSVVANTGNIYAGLYYPIAVAAATFIVGSLLLKETHSTLIWKELETDTPEGAVSDMA
                     GPA"
     gene            complement(5359..5898)
                     /locus_tag="AC159P_03185"
     CDS             complement(5359..5898)
                     /locus_tag="AC159P_03185"
                     /inference="COORDINATES: protein motif:HMM:NF012521.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase family protein"
                     /protein_id="HIR3694488.1"
                     /translation="MRRGVCVFILLLSFAAVSCDRPQAGSTVVLIVRHAEKASDAADS
                     PLTEAGHQRAQALVRAAEEAGVGAIYSTQFRRNLDTARPVSERLGVAVTQTPIADLSQ
                     PGDYGKVLAGEIVSKHRGRTVLVVSHGNTIAGVIEGLSGRAPALSSVEYHDLFVVTVP
                     PTGPAGLIKAQYGQRVGGS"
     gene            6111..6839
                     /locus_tag="AC159P_03190"
     CDS             6111..6839
                     /locus_tag="AC159P_03190"
                     /inference="COORDINATES: protein motif:HMM:NF003992.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HIR3694489.1"
                     /translation="MDGMKSEKISELTTNRLSVYLRCLNLLAAAGIKTISSQALADQF
                     NMNSAQIRKDLAHFGEFGTRGVGYSVDELRRHLTEILGLDRAHRVGIVGAGNLGTALA
                     NYNGFAASNFRVVALFDNDRAKIGRRVGEAGVAVHDVRRLARVVRDEEIDVAVVAVPA
                     RGAQRVLNQIMSAGVKAVLNFAPVRLHARLGVKVKTVDLTISLESLSYFLARPQVVCI
                     TNPRGSLPYEEIAQREEQELQQQN"
BASE COUNT         1126 a         2277 c         2332 g         1176 t
ORIGIN      
        1 cgtgtcttcg cgcagggcgt cttcggaagg ggctgccgcg aggcccgcgc ccgcctcgat
       61 ctcggcgtcg agcgcggggc tggagagcgc ggccatcttg tcgcgcagct cgatgaccag
      121 ccgctcggcg gtcttgcggc ctacgccggg gatggaagtc aggcgcgcga ggttgttggt
      181 gcggatggcg gtgacgatct cgtcggcgct catccccgac agcatggcga tgccggactt
      241 ggggccgatg ccggtcaccg agatgagcag cgtgaacagc tcgcgctcgc gcggcgtgcg
      301 gaagccgaag agctggagcg tgtcttcgcg gacgtgcgtg tagatgcgga gctggacttg
      361 cgatcccggc tcctccatgt tgaagaaggt cgtgacgggg atggtcacct cgtagccgac
      421 gccgcccacg tcgaggatga cactggtcgc ctgtttctcg cgtaaagttc cggataggtg
      481 cgcaatcatt ctttcgtgtg aaaccgtgac gagagaagaa atttcgggag cgcctcttgt
      541 ttcgggaaag attctagttg aacgccgggg cttcggcaac gcggcagggt agtaggcagt
      601 aggcagttaa gaaaaagaaa gaagaggctt gagcttcatc gtctcaggtc tcttgctttg
      661 atgcttgacg aacgaagggg cttgagactt tacccgccaa gcctctctgc cactttgtcc
      721 tgattgccta ctgcctgctg cctgctgcct actgcttctt gcggtagatg ccgttgaagg
      781 tgacgttgag gccgccgcac ttgaggttgt cgctgacgac gaggtgcggg ccgacgagcc
      841 tgatggtcac gctgcatagg tcttcgttct cttccagctt gaggacgttg cccgcgggcg
      901 cggcctcgaa ctcgacctcg ccgacgtgga cgttgtcgcc ttgcccgcgc cagaacgcct
      961 gaccggagac ggcgagcgcg cccgccttct tgccgcgccg gatgtcgagc gactggcccg
     1021 cgtactccca cgcgccgagc cagcgcgcga ggggcggccc ggcgtccgcc tcggagagcg
     1081 agacctgttc ggcgcgcagc cacccgaccg tctccggccc tcgcgcgggc gcgaaccacg
     1141 cgcacacgta atcgccgagg cggcgcgaga cgacgaccgc gtcgcccgga agcacgtagg
     1201 cccgctgcct gcacttcgcg cccgcgccgg gacagtcttc gctcgcgtcg ccgaaaaagt
     1261 aggcgcgcgc gcccttcgcg ccgacgacgt ggcccacgcg gaagcccgcc gcgtcgtgcg
     1321 tgtagaggcc gttgcggcac cagttctcag ggttgccctc gacctgcgcc gaggcgacca
     1381 cgggcacgct ggcgaagagg acgaagagca gggagagttc gaacttcggt cggctcatgc
     1441 ttctcaagcg gaatgaaagt ggacgatcga ccacgaagag tatcgaggac gattgattct
     1501 cagaccgggg gcgctctacg gacgctcgtt gacgatgaag gcgagcgtga cggagacctg
     1561 cgatatctct tcgaccgaca cctcgatggt cgcgtcgggc cagccgcgcg cggcgagcag
     1621 ttggcggatt atctgcttcg cgcggacgat ttggaccggg tcgtaagggc actccttgca
     1681 gacccggaca ccgctctcgc cgagcgcctt gagcacgtcg gcctcgccaa tgctgctcag
     1741 gcccttgaac gtgaagtcgc ggatgatcgg cagctctttg acgtagaaga tgacgcacac
     1801 cccgccgcgc ggcccctcct cgacggcgac gcgcgtctcg gtcttgtcga agaaagccat
     1861 gtcgagcagg gcttgcaggt cgcgttgcac ctgctcgacg ttgaaccggt cgccgggccg
     1921 cgtgcggagc ttgtcgtaaa tctcttcgtc acgcaggcgg cggttgcctt ctatcaacaa
     1981 atcctcgacg aggcggtagt tgtgcttgtc gctcaggagg gcagtaaagc ggttgttgtc
     2041 gctgccgccc aggcgggcaa gcgcgctgcc gccaaaggtg ttgaagttgt actcgaatct
     2101 gatgtgcgtg gcgacccggc gaccgtcctt ctcggcgggc gtgaactcca cgcggctggc
     2161 ggcggcgacg gagatctccg tcagcccgaa gggcaagctt ttcatgattc ggatgtcggt
     2221 gacgcggccc gtgtggcaga gcaccgcggc cagcgcgacg gtgccgctcg cgttgttcga
     2281 gcgggcctct tcggtgaagc cggggtcggg cttatgcttg atgacgggct tgcgagagac
     2341 ctcggtcatc ttgtagaccg gcccgtcgca cttctcttcg ggggtggcgg ggttcgcccc
     2401 ctgcgcggcg gcgggcgcga cggcgaggag cagttgggcg cataggacaa gcactctctt
     2461 catcgctcca ctcctttgaa gaaaacgggc gactccctct ggtgctgtag cccttaagac
     2521 gcgcgtcggg gcgaagaggg ttgaccggcg cccgcttcat ttcgtgaggc ggttgtattt
     2581 tctgagcgct tcgcgcagcc gttcgtgcgg gagggcgtgg gcggtgttgc cgtcgatgcc
     2641 gcgcatcgtc tcggcggcga ccatcgcgtt gacgatggct tcctcggtcg cgtccgcggc
     2701 ggcgcgcagg agcgggttga tgcgctcgtt ggggagcatc gtgagctgcg cggtgccttc
     2761 cgttttgccg acttcggggt tggcggtcga gaaggcgatg aagatgtcgc ccgaactgtt
     2821 ctcgccgcgc ccgcccatca cgccgacgcc gagcgcgacg cggcggacga ggcgcttcaa
     2881 ctggtgcggc agcagcggcg cgtccgtcgc caagacgacg atgatcgaac ccgcgccctc
     2941 gtcggctccg ccgccttcgg cgaccggctt gccgcgctcg ggcagcagat ccgtgatctc
     3001 tctgccgacc ggcacgcccg cgatgcgcag caactggcgg ctgccgtagt tggcctgcac
     3061 gagcacgccc aacgtgtagc cgcccgcctc cgccccgagc atgcgcgaag aggtgccgat
     3121 gccgcccttg aagccgtgcg cgatcatgcc cgtgccgccg ccgacgttgc cttcggcgac
     3181 cggcccgccc cgcgccgaat cgagcgcctg aaaggcgtgc tcttttttga cgtggaagcc
     3241 gttgatgtcg ttcaggaatc cgtcccacgt ctcggcgacg accggcaggc tgaagtcgcc
     3301 ggagtagccc tcgcccgtgc cgcgccgcgc gttccactcg atgaccgcgt cgcgcacgac
     3361 gccgacgctg tgcgtgttgg tgatgaggac gggcgcgccc agtgcgcccg actcttcgag
     3421 ccacgtggtg ccggtcatct cgccgttgcc gttgagcgtg tgccacgcgc cgaagacgcg
     3481 cccacggaaa gtcttcccct gcggcagaat cgccgtcacg cccgtccgca cggggccgcg
     3541 gcccgccacg agcttgcctt cgcccgagac gagcgtcgtg tgcccgacct cgacgccctt
     3601 cacgtccgtg atcgcgttca gggggccggg cgtgccctcg aacggcacgc cgaggtcgcg
     3661 cgcccgcggc ttcgtctgcg cgcgcgcgcc gtgcgcggca aagagcagca cgcacaacgc
     3721 gacgcgcgcc gcgagagcga acggcgccgc gccgcgcgcg aacgtgaacg acgggtcggc
     3781 ttctcttttc acgcttctct tcttgtcacc attttcagac ggcttctcac gccgggccgg
     3841 ccatgtcgct gacggctcct tccggcgtgt cggtctcaag ctctttccag atgagcgtgc
     3901 tgtgcgtctc tttgaggagc agcgagccga cgatgaaggt cgccgccgcg accgcgatcg
     3961 ggtagtacag ccccgcgtag atgttgcccg tgttggcgac gaccgagagg ccgatgaccg
     4021 ggagcagccc gccgaagatg ccgttgccga tgtggtaggg gagcgagagc gccgtgtacc
     4081 tgatcttcgc cgggaacgac tcgacgagga aggccgcgat ggggccgtag accatcgtca
     4141 cgaagacgac ctgcacgaag acgagcagga tcagcttcca cgccgtcgcg ttcgtcaaaa
     4201 ggctgccgat gtcggcgtac ggcaaaacct ccggcgcggg cacgagcgcg ccgttcacct
     4261 ccgccagcgg cgtcagcgtc gccgcgcctg tcacacggtc gggccgcgaa gccgccgtca
     4321 cgacgttcga gcctgccgcc gcctgcatcg ctttataaat cgggatgtac gagacggcgg
     4381 cgagcaggca gcccaccatg atgatccact tgcgcccgat gcggtcggag agcgcgccga
     4441 agaagacgaa cagcggcatc cccaacagca gcgccgcggc cacgatgtac ttcgccgacg
     4501 tggggttcac acgcaggatg gtttccagat agaagagcgc gtagaactgc cctgcgtacc
     4561 acaccacgcc ctgcccggcg gtcgcgccga agagcgagat gagcacgagt ttcaaattcg
     4621 accacttcgt gaacgcgtcc ttcaaaggct gggccgaggt catccccgcg ctcttgatgt
     4681 gactgaagat cggcgactcc ttcatcttca ggcggatgta gagcgacacg ccgaccagca
     4741 ggatcgagat gaggaacggc acgcgccagc cccacgccgt gaacgcctcg cggctgagcg
     4801 cgttctgcac aaggaggata acggcgagcg aaacgaacag cccgagcgtc gccgtgatct
     4861 ggatgaagct cgtgtagaac ccgcgcttgc cgtccggcac gtgctccgcc acgtagaccg
     4921 ccgcgcctcc gtactcgccc ccgagcgcca gcccttgcag cacgcggatc aacagcagca
     4981 ggagcggcgc cgcgatcccg atgctctcgt acgtcggcag gatgccgatg cacgcggtcg
     5041 ccccgcccat gatgagcagc gtcacgagga acgcgtactt gcgccccacc aggtcgccga
     5101 tccgcccgaa gaagagcgcg ccgaacgggc gcacgacgaa ccccacggcg aacgtcgaaa
     5161 gatacgcgat gagcgccagc gtgtcgttgc cctgcgggta gaagagcggc gagatggtcg
     5221 tggccagact cccgaagatg tagaagtcgt accactcgat catcgtcccc accgcggacg
     5281 cggcgatgac ctgccagatt ttgtaatcgc ccgtgccgcc gcgcgcggcg gcgcccgccg
     5341 ccgcggaact gttttgtgtc atgagcctcc taccctctgg ccgtactgcg ccttgattaa
     5401 cccggctggg ccggtcggcg gaaccgtgac cacgaagagg tcgtggtact cgacggagct
     5461 gagcgcgggc gcccgcccgc tcaggccctc gatcacgccg gcgatggtgt tgccgtggct
     5521 gacgaccagc accgtccggc ccctgtgctt cgagacgatc tccccggcga gcaccttgcc
     5581 gtaatcgccc ggctgcgaca ggtctgcgat gggcgtctgc gtgacggcga cgccgagccg
     5641 ctccgagacg ggccgcgcgg tgtctaggtt gcgcctgaac tgcgtgctgt agatggcgcc
     5701 cacgcccgcc tcttcagccg cgcgaacgag cgcctgcgcg cgctgatggc ccgcttcggt
     5761 gagcgggctg tccgcggcgt ccgaagcctt ctccgcgtgc cgcacgatca gcacgacggt
     5821 cgaacccgcc tgcgggcggt cgcacgagac cgccgcgaaa gagagcaaaa gtatgaagac
     5881 gcagacccct cttctcatcc cgtttcctta cgcttcgaat gacagacttt gggcgcctgc
     5941 gggtctcgcg gcccgcgccc tccggttgtg gaacgtaacg cgcgtggacg cattataagg
     6001 tgaaggcgcg tcgaatgggg catgaatttt ccggcgggcg acgcccgcgc cgcgcgtgtc
     6061 tttccccggc gggcgcgggc gcggtatact tgcggcgctc tcccagagaa atggacggca
     6121 tgaaatcgga gaagatttcg gaactgacga cgaaccgttt gagcgtctac ctgcggtgcc
     6181 tgaacctgct ggccgccgcc gggataaaga ccatctcgtc gcaggcgctg gccgaccagt
     6241 tcaacatgaa ctcggcgcag atcaggaaag acctcgcgca cttcggcgag ttcgggacgc
     6301 gcggcgtcgg ctactcggtg gacgagctgc gccgccacct gacagagatc ctcggcctcg
     6361 accgcgcgca ccgcgtcggc atcgtcggcg cggggaacct cggcaccgcg ctcgccaact
     6421 acaacggctt cgccgcgtcg aacttccgcg tcgtcgcgct cttcgacaac gaccgcgcga
     6481 agatcggacg gcgcgtcggc gaggcgggcg tcgcggtgca tgacgtgcga cggctcgcgc
     6541 gcgtcgtgcg cgacgaagag atcgacgtgg cggtggtcgc cgtgccggcg cgcggcgcgc
     6601 agagggtctt gaaccagatc atgtcggcgg gcgtcaaggc cgtgctcaac ttcgcgcccg
     6661 tccgcttgca tgcgcggctc ggcgtgaagg tgaagaccgt ggacttgacc atctcgctcg
     6721 aatcgctgtc gtacttcctc gcacgcccgc aggtcgtctg catcaccaac ccgcgcggca
     6781 gcctccccta cgaggagatc gcgcagcgcg aggagcagga gttgcagcag cagaactgac
     6841 aaggagtttc gtgatgtacc tgttcttact caagaaggca cttccgttca cgctgacctt
     6901 catcttcggc g
//
LOCUS       DBNGOH010000173         8149 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4790898, whole genome shotgun sequence.
ACCESSION   DBNGOH010000173 DBNGOH010000000
VERSION     DBNGOH010000173.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8149)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8149)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8149
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4790898"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            97..1602
                     /locus_tag="AC159P_03195"
     CDS             97..1602
                     /locus_tag="AC159P_03195"
                     /inference="COORDINATES: protein motif:HMM:NF013702.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HIR3694490.1"
                     /translation="MPDEVKVLRLRDMVLRRRGTVARRLMHAWISVALRLFFRRIETA
                     NARVVPQAGPLVFVINHPNGLIDPGLVFTALPRRISFLAKSTLFRLPVVGALLGAVEA
                     LPLYRRVDEGEDMNRNRRTFEACRRLLRRGRCIALFPEGKSHNEPRLLPVKTGAARIA
                     LGARAVEDDDDDEGAAVSVDERDAGGDASAVDEAGAASPSSRGFDLKIVPVGLYYTSK
                     TSFRSEALLRFGAPLEVPQVVLDEQGEPPHEAVRLLSARIEEALRGVTLNAEDEGQLD
                     VATKAERLFSSLYEGLNVELPLASKFDFLRRFIEARLVGGASAPRGADALRGRILGYA
                     EELRRIGIEPENLSLSRHSRWYVFRHFLLRAGLLAALLPLTVVGAVVHLPAYLLSTLA
                     ARLYTKHGVDEVAPTVKILAAIVLMPLTWLVVGAVAFFAWGWRAALLALPLSILCGYA
                     AMRSLEEAYDMRGWYKAVVLLFRRRQLFLRLLLEREALRGEITRFEKRAGG"
     gene            1735..2463
                     /gene="pepE"
                     /locus_tag="AC159P_03200"
     CDS             1735..2463
                     /gene="pepE"
                     /locus_tag="AC159P_03200"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016869735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HIR3694491.1"
                     /translation="MGKRLFLLSNGSELVGEHANEEMVGALRDFLGEGVGRLLLIPYA
                     SVIRTYDESAALARSRLAPLGYEIESVHESHDPRAAVALAEAIVIHGGNTFRLLSELH
                     ANNLVGAIRARVEAGTPYVGWSAGANVACPTIRTTNDMPVVEPQSFAALSLVPFQINP
                     HYTDRALDGHHGETRDERIAEFVHLNPGVYVVGLREGTTLRVEDSGVRLVGDKPARVF
                     LHGRTPHELGPADSFDFLLGGDAA"
     gene            2469..3317
                     /locus_tag="AC159P_03205"
     CDS             2469..3317
                     /locus_tag="AC159P_03205"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009393073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HIR3694492.1"
                     /translation="MRQYQDLLKTVLARGTPHGDRTGVGTVSYFGHQTRFDLRAGFPI
                     VTTKRVPFRWVAEELFWFLSGDTNEATLRARGVDIWKEWADLAHTARFGREEGDLGPV
                     YGYLWRSFGGHYPRRDGVDQIARLVEEIRRDPNSRRLIVTGWDPRVATEVDLPPCHTL
                     FQFKVERGRLLHCQLYQRSADAFLGVPFNISSYALLTHLLAHVCGLEVGDFVHTFGDL
                     HIYSNHLAQVEELLAREPLPLPRLEIEDEGGRLRGLEGLLNFRYEHLKLVGYKSHGKI
                     EAPVAV"
     gene            3435..3911
                     /locus_tag="AC159P_03210"
     CDS             3435..3911
                     /locus_tag="AC159P_03210"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF012413.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HIR3694493.1"
                     /translation="MAIIGIVAVDRAGAIGKGGSLPWHYSGDLKHFKEQTSGHAVVMG
                     RRTWAGLKRPLPNRLNIVLSRGEGGEAQESVLWLRDRQSVLSLKDYLKGDLYVIGGAQ
                     VFDVFRDDIERWVVTEVPLTVEGADTFMPAGFLDGFEAYDSRRLDENRTARFYRLA"
     gene            complement(3961..5844)
                     /locus_tag="AC159P_03215"
     CDS             complement(3961..5844)
                     /locus_tag="AC159P_03215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694494.1"
                     /translation="MSDADQRRRFVDDARTRALGPPPERPAEAGRPDAGARSRAAAQL
                     QPLDVGRALARELFARVPNADPLVAAVAPGFAAILQGAVKQARAPTSSVGALLAPLIQ
                     SGAPPPPVPGDEDEQNPQAAVIARLLAPVVGAALTAATPAALPLTVAGAALTAATLRT
                     DFDALAQTEPFKTVYQALKKSGVIDLAGTSLGGGTLAASFYTPDKVRRVLRSLAKFLE
                     PVVGEVVTYVEEELKQAALRSVLAEAVSRLSLALPFSVKSNRDIGNHVHKELQEGYVR
                     LYSKTRDIVVERCTPDDRVELFVIGPTTSLVPMPLQDVAIMPARDPLYFFRALRGAMN
                     GFKLHKDSWLRADLVDAYTNEIWEIKPVGNLFGGVWQETLYRVSFNVIRMIVLVGQPE
                     PLRLAHFMRGGDFWFDFTRRAFGNEVTEIALHQQITVTRHAGRPAVATPFQVGALPGL
                     IGYFVLQGPNVPRFQEVLETILIPALLAAIAKILKDLEKLGERLGEAVAEARRVLGEM
                     AEALKRFAEAIWPFVVAIAVVGAFIAFFPAGLGGLAVAGAAALIILIVGEVVGEDSGD
                     GPGKGGGDRDGKPTDVRIGPVMVRGVPRSKVPQFLAEVQRQMHDIVGAAADELQRRPP
                     AVA"
     gene            complement(5893..>8149)
                     /locus_tag="AC159P_03220"
     CDS             complement(5893..>8149)
                     /locus_tag="AC159P_03220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694495.1"
                     /translation="ALPANIIRDDYSITRLVYLVRPDGAGIPVPVVLPRAVANKTIAE
                     RLSADALKAISGGQPLLSKWGAALQTLQAGGGVRIPAPPQVSPAQARDLLPDFELALY
                     LPYRQTWELLGYSRGALLNSISLAPQEETTIEIFSWERIKRTREETVSTEQETSQETA
                     LTDKDSREVLKELTKDSSFTFHAGLDLQIPVGNVPIGVDVGVETRDSLRDFSRATQTK
                     VNEAVRKASVRMKTSRQTKVTETEEFGRETKVSRKLRNPNMCRALNIDMFEVLSNYTV
                     TTTLLRERAALCVLIPIPINMEITREFLLVHEGVLRRTLLSAVYANGFEAARLLATWQ
                     RLCEVKCAPPCPCEQPAGVPSDDPVVEEARQRVAVAADAVAASIDTVNQAGPGDLCDL
                     ACFVTSGDESVWAAAKLQFHQWLYAKLMNLIASRWWSDCRQFAQSTDRSPEAADRLLQ
                     SANAQPADIFNLILLGIRFFATAAEIVVTLVAEGCWNVWVLITNIAFHDAGLDSAIRQ
                     LRTAVAAYHAAVAGSLPIGSPPEGPAPPPAPEFPPRELARALVDEAALIAHINGNTAY
                     YRFAIWEALALNDRTALIAGQTDVLPFVEPDVLGYVGSKIALRFRLAADADALEWFQG
                     NVLDNPGLQEPPQPFPVTLQTPGVMTEPRLGQCDACEEFIMKHRELDVQRMTHEIVAA
                     QQRAEMEKFEAQRYQMRLAQKPPMLEDPDLNRETGAIRVILQDDRPGAGTTGTTTGTT
                     TGTTTVETTPE"
BASE COUNT         1227 a         2748 c         2810 g         1364 t
ORIGIN      
        1 tttattacac cgaggcgaaa tgtcaaacac ttttgcgcgg cccgacctct cgcgccctcc
       61 gcggggtcgc ggggcgaatg aggctataat ccacggatgc cggatgaggt gaaggttctg
      121 cgcctccgcg acatggtgct gcgtcgccgg gggacggtgg cgcggcggct gatgcacgcg
      181 tggatcagcg tggcgctgcg tctgttcttc cgccggatcg agacggcgaa cgcgcgcgtg
      241 gtgccgcagg cggggccgct cgtgttcgtg attaatcacc cgaacgggtt gatcgacccc
      301 ggcctcgtct tcacggcgct gccgcgacgc atctccttcc tcgccaagtc cacgctgttc
      361 cgcctgcccg tcgtcggcgc gctgctcggc gcggtcgagg cgctgccgct ctaccgccgc
      421 gtggacgagg gtgaggacat gaaccggaac cgccgcacgt tcgaggcgtg ccgcagactg
      481 ctgcggcgcg ggcgctgcat cgcgctcttc cccgaaggca agtcgcacaa cgagccgcgg
      541 ctgctgccgg tcaagactgg cgcggcgcgc atcgcgctcg gggcgcgcgc cgtcgaggat
      601 gacgacgacg atgaaggcgc cgccgtctct gttgatgaga gggacgccgg cggagacgcg
      661 agcgcggtcg atgaagcggg tgctgcgtcg ccgtcgtcgc gcgggttcga tttgaagatc
      721 gtgccggtgg ggctgtacta cacttcgaag acctcgttcc ggagcgaggc gctgttaagg
      781 ttcggggcgc cgctggaggt gccgcaggtc gtgctcgacg agcagggcga gccgccgcac
      841 gaggcggtca gacttttgtc ggcgcgcatc gaggaggcgc tgcgcggcgt gactctgaac
      901 gccgaagacg aggggcagct cgacgtggcg acgaaggccg agcggctctt ctcttcgctc
      961 tacgaggggc tgaacgtcga gctgccgctg gcgtcgaagt tcgacttcct gcgccgcttc
     1021 atcgaggcgc gtctggtcgg cggcgcgtcc gcgccccgcg gcgctgatgc tttgcgcggg
     1081 cgcatcctcg gctacgcgga agagctgcgg cgcatcggca tcgagccgga gaacctgtcg
     1141 ctctcgcgcc actcgcgttg gtacgtcttc cgccacttcc tcttgcgcgc ggggctgctc
     1201 gcggcgctgc tcccgctgac ggtcgtgggc gccgtcgtgc acctgccggc gtacctgctg
     1261 agtacgctcg cggcgcggct ctacacgaag cacggcgtgg acgaggtggc gccgacggtc
     1321 aagattctgg cggccatcgt gctgatgccc ctgacgtggc tcgtcgtcgg cgccgtcgcg
     1381 ttcttcgcgt ggggctggcg cgccgccctc ctcgcgctcc cgctgtcgat cctgtgcggc
     1441 tacgcggcga tgcgctcgct cgaagaggct tacgacatgc gcggctggta caaggccgtc
     1501 gtgctgctct tccgtcggcg gcaactgttt ctgcggctgt tgctcgaacg cgaggcgctg
     1561 cgcggcgaga tcacgcgctt cgagaaacgc gcgggcggct gaacgatgtt aagatgcgaa
     1621 gagaaatcag aagtcagaag tcagaagtca gaatgcaaaa gatgcatgaa ggcggtttgc
     1681 ttctcactca ccattcatcg ctgctgactt ctgacttctt cggggtgtga gactgtgggc
     1741 aaaagacttt tcctgctgag caacgggtcg gaactcgtcg gcgagcacgc gaacgaggag
     1801 atggtgggcg cgctcaggga cttcctcggc gaaggcgtcg ggcgcctgct cctgatccct
     1861 tacgcttcgg tcatccgcac ctacgacgag agcgcggcgc tcgcgcgctc gcgcctcgcc
     1921 ccgctcggtt acgagatcga gtccgtccac gagtcgcacg acccgcgcgc ggcggtcgcg
     1981 ctcgcagagg ccatcgtcat ccacggcggc aacacgttcc ggctgctctc cgaactccac
     2041 gcgaacaacc tcgtcggcgc gatccgcgcg cgcgtcgagg ccggcacgcc gtacgtcggg
     2101 tggagcgcgg gcgccaacgt cgcgtgcccg accatccgga cgacgaacga tatgccggtc
     2161 gtcgagccgc agagcttcgc ggcgctctcg ctcgtcccct tccagatcaa cccgcactac
     2221 acggatcgcg cgctcgacgg ccaccacggc gagacgcgcg acgagcgcat cgccgagttc
     2281 gtccacctca accccggcgt ctacgtcgtc ggcctgcgcg agggcacgac gctgcgggtc
     2341 gaagactccg gcgtccggct cgtcggcgac aagcccgcgc gcgtcttcct gcacggcagg
     2401 acgccacacg aactcggccc cgcggactcc ttcgacttct tgctcggcgg cgacgcagcc
     2461 tgaaacttat gagacagtat caagacctgc tcaagaccgt cctcgcgcgc ggcacgccgc
     2521 acggcgaccg caccggcgtc ggcaccgtct cctacttcgg ccaccagacg cgcttcgatt
     2581 tgcgcgcggg cttccccatc gtcaccacca agcgcgtccc cttccgctgg gtcgcggaag
     2641 agctgttctg gttcctttcg ggcgacacga acgaagcgac tttgcgcgcg cgcggcgtgg
     2701 acatctggaa ggagtgggcc gacctcgcac acaccgcccg cttcgggcgc gaggagggcg
     2761 acctcggccc cgtctacggc tacctctggc gctcgttcgg cggccactac ccgcgacgcg
     2821 acggcgtcga tcagatcgcc cgcctcgtcg aagagatacg gcgcgacccc aactcgcgcc
     2881 gcctcatcgt cacgggctgg gacccgcgcg tggcgaccga ggttgacctg ccgccgtgcc
     2941 acacgctctt ccagttcaag gtcgagcgcg ggcgcctgct ccactgccag ctctaccagc
     3001 gctcggcgga cgccttcctc ggcgtgccct tcaacatcag ctcctacgcg ctgctcacgc
     3061 acctgctcgc gcacgtctgc gggctggagg tcggcgactt cgtccacacc ttcggcgacc
     3121 tgcacatcta cagcaaccac ctcgcgcagg tcgaagagct gctggcgcgc gagccgctgc
     3181 cgctgccgcg cctcgaaatc gaggacgagg gcgggcgttt acgcgggctg gaagggctgc
     3241 tgaactttcg ctacgagcac ctgaagctgg tcggctacaa gtcgcacggc aagatcgagg
     3301 cgccggtcgc cgtctgaaga gatgatgaat gatgaatgat gaatgcggaa tgatgaacgg
     3361 gaagacgcgt gcttctcatc atcgctcttc ttctcattca tcattcatca ttccgcattc
     3421 atcattttct tgatatggca atcatcggca tcgtcgctgt tgaccgcgcg ggcgccatcg
     3481 ggaagggcgg ctcgctgccg tggcactact ccggcgacct gaagcatttc aaggagcaga
     3541 cgagcggcca cgccgtcgtg atgggccgca ggacgtgggc gggcttgaag aggccgctgc
     3601 cgaaccggct gaacatcgtc ctcagcaggg gcgaaggggg cgaggcgcag gagtccgtgt
     3661 tgtggctgcg cgaccggcag tcggtgctgt ctttgaagga ttatttgaag ggcgacctct
     3721 acgtcatcgg cggcgcgcag gtcttcgacg tgttccgcga cgacatcgag cggtgggtcg
     3781 tcaccgaagt cccgctgacg gtcgaaggcg cggacacgtt catgccggcg ggcttcctcg
     3841 acggcttcga agcctatgac tcgcgccgcc tcgacgagaa ccggacggcg aggttctacc
     3901 ggctcgcgta ggccgtaagg gcgcgcgtcc ggcggtcgcg accgccgggc cgccgccccg
     3961 tcacgccacg gcgggcgggc gccgctgcaa ctcgtccgcg gcggcgccca cgatgtcgtg
     4021 catctgccgc tgcacctccg cgaggaactg cggcaccttc gaccgcggaa ctccgcgaac
     4081 catcaccggg ccgattcgaa cgtcggtcgg tttgccgtca cgatcgccgc cgcccttgcc
     4141 cggcccgtcg ccggagtcct cgccgaccac ctcgccgaca atcagtatga tgagcgcggc
     4201 ggcgcccgct acggccaagc cccccaagcc ggcggggaag aacgcgatga atgcgccgac
     4261 gaccgcaatc gccacgacga acggccagat ggcttcggcg aagcgcttga gcgcctcggc
     4321 catttccccg aggacgcgcc gcgcctccgc gacggcctcg cccagacgtt cgccgagctt
     4381 ttcgagatcc ttcaaaatct tcgcgatcgc tgccagcagc gccgggatga ggatggtttc
     4441 gagcacttcc tggaacctgg gcacgttcgg cccctggagc acgaagtagc cgatgaggcc
     4501 cggcaaggcg ccgacctgga acggggtggc gacggcgggc cggcctgcat gccttgtgac
     4561 ggtgatctgt tggtgcaagg cgatctccgt gacctcgttg ccgaacgccc tacgtgtgaa
     4621 gtcgaaccag aagtcgccgc cgcgcatgaa gtgcgccagg cgaagaggtt ccggctgccc
     4681 gacgagcacg atcatgcgga tcacgttgaa cgacacgcga tagagcgtct cctgccagac
     4741 gccgccgaag aggttgccca ccggcttgat ctcccaaatc tcgttcgtat atgcgtccac
     4801 cagatcggcg cgtagccacg agtccttgtg cagcttgaac ccgttcatcg cgccgcgcag
     4861 cgcccggaag aaatagagcg ggtcccgtgc gggcatgatc gcgacatcct gtagcggcat
     4921 cggcaccagg ctcgtcgtgg ggccgatgac gaacagctcg acgcggtcgt cgggggtgca
     4981 gcgctcgacc acgatgtccc tcgtcttcga gtaaagcctg acgtaaccct cctgtagctc
     5041 cttgtgcaca tggttgccga tgtccctgtt cgacttgacg gagaagggca gggccaggct
     5101 caaccggcta accgcttcgg cgagcacgct gcggagcgcc gcctgcttga gttcctcttc
     5161 gacgtacgtg actacctccc ccaccactgg ttcgaggaac tttgccagcg agcgcagcac
     5221 gcgacgcacc ttgtcggggg tgtagaacga ggcggccagc gtcccgccgc ccagtgacgt
     5281 gccggccaaa tcaatcacac cgctcttttt tagcgcctgg tagacggtct tgaacggctc
     5341 ggtctgcgcg agcgcgtcga agtccgtccg cagcgttgcc gccgtcagcg ccgcgccggc
     5401 caccgtgagc ggtaaagccg ccggcgtcgc cgccgtcagc gcggcgccga cgaccggcgc
     5461 gagcagcctg gcgatgacgg cggcctgcgg gttctgttcg tcctcatcgc ccgggacggg
     5521 cggcggcggc gcccccgact gaatcagcgg cgccagcaac gccccgacgg aactcgtcgg
     5581 cgcgcgcgcc tgtttgacgg ccccctgtag gatggccgcg aatcccggcg ccaccgccgc
     5641 gacgagcggg tcggcgttcg gcactctcgc gaacagctcg cgcgccaacg cgcgccccac
     5701 gtcgaggggt tgaagctgcg cggcggcccg gctcctcgcc cccgcgtccg gtcggcccgc
     5761 ttccgcgggg cgctcgggcg gcggcccgag cgcccgcgtg cgggcatcat ccacgaaccg
     5821 tctgcgctgg tcggcgtcac tcatagcaaa cctccgtccc gtcatgcccc gccggcgggc
     5881 gggagagcgt gctcactccg gtgtcgtctc cacggtggtg gttcccgtgg tggtgccggt
     5941 ggtggtgccg gtagtgccgg cgcccggcct gtcgtcctga aggatgacgc ggatggcgcc
     6001 ggtctcgcgg ttcaggtcgg ggtcttcgag catcggcggc ttctgcgcga ggcgcatctg
     6061 gtagcgctgc gcttcgaact tctccatctc ggcgcgctgc tgcgcggcga cgatctcgtg
     6121 cgtcatgcgc tgcacgtcga gttcgcggtg cttcatgatg aactcctcgc aggcgtcgca
     6181 ctggccgagc cgcggctccg tcatcacgcc gggcgtctgg agggtgacgg ggaagggctg
     6241 cggcggctcc tgtaggccgg ggttgtccag cacgttgccc tggaaccatt cgagggcatc
     6301 ggcgtcagcc gcgaggcgga agcggagggc gatcttactc cccacgtagc cgagcacgtc
     6361 cggctcgacg aagggcagca cgtcggtctg cccggcgatc agcgccgtgc ggtcgttcag
     6421 cgccagcgcc tcccagatgg cgaaccggta ataggccgtg ttgccgttga tgtgtgcgat
     6481 gagcgccgcc tcgtccacca aagcccgcgc cagctcgcgc ggcgggaact cgggggccgg
     6541 gggcggcgcg ggaccctccg gcgggctgcc gatcggcagc gaccccgcga ccgccgcgtg
     6601 gtaggccgcg accgccgtgc ggagttgacg gatggcgctg tcgaggccgg cgtcgtggaa
     6661 agcgatgttg gtgatgagca cccagacgtt ccagcagccc tcggcgacga gcgtgacgac
     6721 gatctccgcg gcggtggcga agaacctgat cccgagcagg atcaggttga agatgtccgc
     6781 cggctgcgcg ttggcggact ggagcagtcg gtcggcggcc tccggcgagc gatcggtgct
     6841 ctgcgcgaac tggcggcagt cagaccacca gcgcgacgcg atcaggttca tcagcttggc
     6901 gtagagccac tggtggaact gtagcttggc ggcggcccac acgctttcat cccccgaggt
     6961 gacgaagcag gccaggtcgc agaggtctcc cgggccggcc tggttgaccg tgtcgatcga
     7021 ggcggcgacc gcgtcggcgg cgaccgcgac ccgctggcga gcctcctcga ccacggggtc
     7081 gtccgaaggg acgcccgccg gctgctcgca cgggcagggc ggcgcgcact tcacctcgca
     7141 gagccgctgc caggtggcga gcaatcgcgc cgcctcgaag ccgttggcgt agaccgccga
     7201 gagcagcgtg cgccgcagca ccccctcgtg gacgaggagg aactcgcggg tgatctccat
     7261 gttgatgggg atcgggatga gcacgcacag ggcggcgcgc tcgcgcagca gcgtggtcgt
     7321 cacggtgtag ttcgacagca cctcgaacat atcgatgttg agcgcccggc acatgttcgg
     7381 gttgcggagc ttgcgcgaga ccttggtctc gcgcccgaac tcctccgtct cggtgacctt
     7441 cgtctgccgg ctcgtcttca tccgcacgct ggccttgcgg acggcctcgt tgaccttcgt
     7501 ctgggtggcg cgcgagaagt cgcgcaacga gtcgcgcgtt tcgacgccca catcgacgcc
     7561 gatggggaca ttgcccaccg gaatctgaag gtcgaggccg gcgtggaacg tgaagctgga
     7621 atccttggtc agctctttga gcacctcgcg gctgtccttg tcggtgaggg cggtctcctg
     7681 cgaggtctcc tgctcggtgc tgaccgtctc ctcgcgcgtg cgcttgatcc gctcccagct
     7741 aaagatttcg atggtcgtct cctcctgcgg ggccagcgag atcgagttga ggagcgcgcc
     7801 gcgcgagtag ccgagcagtt cccaggtctg ccggtacggc aggtagaggg caagctcgaa
     7861 gtccggaagc aggtcgcgcg cctgggcggg gctgacctgc ggcggggcgg ggatacggac
     7921 gcccccgccg gcctgtaggg tttggagcgc cgcgccccac ttcgacagca ggggctgacc
     7981 gccgctgatc gctttcagcg cgtcagcaga gaggcgctcg gcgatggtct tgttggcgac
     8041 ggcgcgcggc agtacgacgg gcacgggaat gcccgcgccg tccggcctca ccagatagac
     8101 gaggcgcgtg atgctgtagt cgtctcggat gatgttggcc ggcagcgcg
//
LOCUS       DBNGOH010000174         3706 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1491584, whole genome shotgun sequence.
ACCESSION   DBNGOH010000174 DBNGOH010000000
VERSION     DBNGOH010000174.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3706)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3706)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3706
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1491584"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..321
                     /locus_tag="AC159P_03225"
     CDS             <1..321
                     /locus_tag="AC159P_03225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694496.1"
                     /translation="GSTFGYSSASEKKATLSGWKGKDPIKVSGGRCTTTAKSVGDESV
                     GEAGRQLLAKGLQRLLDAEKSCGLITKVFRASRNHERDQPAEAWVAEFERKARTIIDA
                     RCSN"
     gene            487..1137
                     /locus_tag="AC159P_03230"
     CDS             487..1137
                     /locus_tag="AC159P_03230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694497.1"
                     /translation="MRADLHNRRMTLYGVARIIIAAALAVVLVSGAAPLGSASAGHLC
                     AMACCAGQPPHEAGSCTHGSCQVSFATHESPAPPEEPEELCGAPQPRSERHRAAPAPP
                     TPPAHAEDVSPSSHHQHHAQADSAAREHSHRGDSRQPPFFSASVLDKPCPPDCGAATS
                     VYSSQSRPRDSAAVSHADRARPPSGPRPRQTSYHSAGELDALCRRSRPRGPPTSFS"
     gene            1299..>3706
                     /locus_tag="AC159P_03235"
     CDS             1299..>3706
                     /locus_tag="AC159P_03235"
                     /inference="COORDINATES: protein motif:HMM:NF051698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR3694498.1"
                     /translation="MPLDHLNGLRPPQAAAVLILSFFLFITGGPRPLAQQSVTSATLS
                     GRVSDADDAAVGGASVTATNLDTNQKQTATSDAEGRYRFSYLSVGTYRLAAEAAGFAP
                     HERRNTLTVGQALDVPLRLAVANVAESIDVTTAEPVVETVRTQLAETIRPREIDDLPL
                     NGRNFLDLALLVPGVSRTNTGSNQRFAETSAVPGQGLSVAGQRNLNNSFVVDGLSAND
                     DAADLTGTYYSQEVIREFQVVTSGGIAEFGRASSGVVFVVTQSGTNDWRSRLYGFLRN
                     QRFDARHPLAPRKDALTQGQYGASAGGPLRRDRTFLFANFEQTRRHDSVVITIAPANV
                     ASINNRLALINYPGQRVETGLVPGGHDSTNFFARVDHRLDASNLLAARYSLYRISATN
                     SRNVGGLNADGRGTALGNSDQTVAASNVTTVSSRTLNEARFQFTRSRLDAPVNDATGP
                     AVNIAGVANFGTATFSPLARDIDLYELVDNVSTQRGAHSLKGGVGLLCNRVDIDFPGA
                     LQGVYTFAGLANFLNGAYVSFQQAFGAPAQVQSNPNLGLFVQDEWRPRPSLTVNAGLR
                     YDVQGLPDPIRTDTNNFAPRVGLAYAPWDRKTVLRAGFGLYFDRIPLRATSNALQRDG
                     SKYLAVQLSPTQTGAPVFPNVLAALPATLPTRPNITRIDPDIENGYGQQLNLQIEREL
                     PGDASFSIGYLRLRGLHLIMSRNVNVPRFHASAGVPNLGRPDASWGNISRFESSGNSH
                     YDGMVVAYNRRAARWSSVRVSYTLSKAIDDAGNFFFSTPQDNFNPRDDRGLSDNDQRH
                     RLVV"
BASE COUNT          582 a         1456 c         1121 g          547 t
ORIGIN      
        1 ggttcgacct tcggctattc aagcgccagc gagaagaagg cgacgctgag cggctggaag
       61 ggcaaagacc cgatcaaggt gagcggcggg cgctgcacca cgaccgccaa gagcgtcggc
      121 gacgagagcg tcggcgaggc gggccgccag ctcctcgcca aaggtcttca gcgcctgctc
      181 gacgccgaga agtcgtgcgg cctcatcacc aaagtattcc gcgcctcgcg caaccacgag
      241 cgcgaccagc ccgccgaggc gtgggtcgcc gagttcgagc gcaaggcccg caccatcatc
      301 gacgcgcgct gctcgaactg aaggtgcgcg cggttgatgc gagatggacg cttttgcgcg
      361 cctcgctcga caacacacgg acgagcggct cgaccggcga gcttttcgtc caactcctcg
      421 cgccgatatc ggtgacaccg gacgcgcttt gtgatacgtt ccgtgcgccg agtttcgcat
      481 accgtcatgc gcgccgattt gcacaacagg cggatgacgc tgtacggcgt cgcgcgaatc
      541 atcatcgcgg cggcgctggc ggtcgtcctt gtgtccggcg ccgccccgct cggctctgcg
      601 tctgcggggc acctctgcgc gatggcctgc tgcgccggcc agcccccgca cgaggccggt
      661 tcgtgcacgc acggctcctg ccaggtcagc ttcgccacac acgaatcgcc cgcgccgccc
      721 gaagagccgg aagaactctg cggggcgcct cagccgcgca gcgaacggca ccgcgccgcg
      781 cccgcgccgc ccacgccgcc cgcgcacgcg gaagacgttt cgccgtcgtc gcaccatcag
      841 catcacgcgc aggcggattc cgctgcgcgc gagcactcgc accggggcga ctcgcggcag
      901 ccgcccttct tcagcgcgag cgtgctcgac aagccgtgcc cgccggactg cggcgccgcc
      961 acgtccgtct attcgagcca gagccgcccg cgcgattccg ccgccgtctc tcacgcggac
     1021 agggcgcggc ccccttccgg cccgcgcccg cggcagactt cataccattc cgccggtgaa
     1081 ctcgacgcgc tgtgtcggcg ctcgcgcccg cgcggcccgc ccacctcttt ctcctgaata
     1141 gaaccagccc cgacgacgca cggcgtgtgt ccgttcggcc cgaagtgtag gcagtcgctc
     1201 taccttcagc ggcgcgttcg gctggccgtg ccataggcgc atcggcagcc tcacgctggt
     1261 cggcgcgtcg tcgcccattt ctaacgggag caggagcgat gccactcgat cacctcaacg
     1321 ggctgcgtcc gccgcaggcg gcagccgtcc tcatcctttc attctttcta ttcattacgg
     1381 gcgggcctcg cccgctcgcg cagcagtccg tcacctcggc cacgctcagc ggtcgcgtct
     1441 cggacgcgga cgacgcggcg gtcggcggcg cgtccgtcac cgcgaccaac ctcgacacga
     1501 accaaaagca gaccgcgacg agcgacgccg aaggccgcta ccgtttctcc tacctctcgg
     1561 tcggcaccta ccggctcgcc gccgaggccg cgggcttcgc cccgcacgaa cggcggaaca
     1621 cgctgaccgt cgggcaggcg ctcgacgtgc cgctccggct cgcggtggcg aacgtcgccg
     1681 agtcgattga cgtgacgacc gccgagccag tcgtcgagac cgtccgcacg cagttggccg
     1741 agaccatccg cccgcgcgag atagacgacc tgccgctcaa cggtcgcaac ttcctcgacc
     1801 tcgcgctgct cgtgcccggc gtctcgcgca ccaacacggg cagcaaccag cgcttcgccg
     1861 aaacctccgc cgtgcccgga cagggactct cggtcgcggg ccagcgcaac ctcaacaaca
     1921 gcttcgtcgt ggacggcctc tcggccaacg acgatgccgc cgacctgacc ggcacttact
     1981 acagccagga ggtgatccgc gagtttcagg tcgtcacctc gggcggcatc gccgagttcg
     2041 gtcgcgcctc ttcgggcgtc gtcttcgtcg tcacgcagtc gggcaccaac gactggcgga
     2101 gccgcctcta cgggttcttg cgcaaccagc gcttcgacgc gcgccacccg ctcgccccgc
     2161 gcaaggacgc gctcacgcaa ggccagtacg gcgcgagcgc gggcggcccc ctgcgccgcg
     2221 accgcacgtt cctcttcgcc aacttcgagc agacgcggcg ccacgactcg gtcgtcatca
     2281 ccatcgcgcc cgccaacgtg gcgtcgatca acaacagact cgcgctgatt aactaccccg
     2341 gccagcgcgt cgagacgggc ctcgtccccg gcggccacga ctcgaccaac ttcttcgcgc
     2401 gcgtcgatca caggctcgac gcctcgaacc tgctcgcggc gcgctacagc ctctaccgca
     2461 tctcggccac caactcgcgc aacgtcggcg gcctcaacgc cgacggtcgc ggcaccgcgc
     2521 tcggcaacag cgaccagacc gtcgccgcga gcaacgtcac caccgtcagc agccgcacgc
     2581 tcaacgaggc gcgcttccag ttcacgcgca gccgcctcga cgcgcccgtc aacgacgcga
     2641 ccggccccgc cgtcaacatc gcgggcgtgg ccaacttcgg caccgccacc ttctcgccgc
     2701 tcgcgcgcga catagacctc tacgagctgg tcgataacgt ctcgacccag cgcggcgcgc
     2761 actccctgaa aggcggcgtc ggtctgctct gcaaccgcgt cgatatcgac ttccccggcg
     2821 cgcttcaggg cgtctacacg ttcgccggcc tcgccaactt tctgaacggc gcctacgtca
     2881 gcttccagca ggcgttcggc gcgcccgcgc aagttcagtc gaacccgaac ctcggcctct
     2941 tcgtgcagga cgagtggcgg ccgcgcccct ctctgaccgt caacgcgggc ttgcgctacg
     3001 acgtgcaagg tctgcccgac ccgatccgga cggacacgaa caacttcgcg ccgcgcgtgg
     3061 ggctggccta cgcgccgtgg gatcgcaaga cggtgttgcg cgcgggcttc ggcctctact
     3121 tcgaccgcat ccctctgcgc gccacctcga acgccttgca gcgcgacggc tcgaagtacc
     3181 tcgccgtcca gctctcgccg acgcagaccg gtgcgcccgt cttcccgaac gtgctcgccg
     3241 cgctgcccgc cacgctcccg acgcggccca acatcacgcg catcgacccg gacatcgaga
     3301 acggctacgg ccagcagctc aacctccaaa tcgagcgcga gctgccgggc gacgcctcct
     3361 tctccatcgg ctacctgcgc ctgcgcggcc tccacctcat catgtcgcgc aacgtcaacg
     3421 tgccgcgctt ccacgcctcc gcgggcgtgc ccaacctcgg gcggcccgac gcaagctggg
     3481 gcaacatctc gcgcttcgag agctcgggca actctcacta cgacgggatg gtcgtcgcct
     3541 acaaccgtcg cgccgcgcgc tggtcttccg tgcgcgtctc ctacacgctc tcgaaggcga
     3601 tagacgacgc gggcaacttc ttcttctcga cgccgcagga caacttcaac ccgcgcgacg
     3661 accgcggcct ctccgacaac gaccagcgcc accgtctggt cgtcag
//
LOCUS       DBNGOH010000175         9054 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3691504, whole genome shotgun sequence.
ACCESSION   DBNGOH010000175 DBNGOH010000000
VERSION     DBNGOH010000175.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 9054)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9054)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9054
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3691504"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..660)
                     /locus_tag="AC159P_03240"
     CDS             complement(<1..660)
                     /locus_tag="AC159P_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009196619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HIR3694499.1"
                     /translation="MKALRSRRRGAPVFLLALLLAASSSFSLQLRAQRTTPASAARAY
                     YPAEEWERRTPQQAGMDAAGLQEAVAFAVAAESKAPRDLELAHYQTFGREPFGHAVGP
                     FKGRGDPTGVVIRHGYVVAEWGDPLRVDMTFSVTKSFLSTTVGLAHDRKLIRSLQDRV
                     RDYMAPVYPLAPNRGARRAEALGEPQLIEPFESEHNRKITWEHLLRQTSDWEGTLWGK
                     PE"
     gene            1069..1233
                     /locus_tag="AC159P_03245"
     CDS             1069..1233
                     /locus_tag="AC159P_03245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694500.1"
                     /translation="MRFGSLAVCSLFVSQTSCIVVGGYSDDGGWFIWPWGLVISAVFI
                     GLFLLLRRRR"
     gene            1250..1789
                     /locus_tag="AC159P_03250"
     CDS             1250..1789
                     /locus_tag="AC159P_03250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694501.1"
                     /translation="MNDGPRQTLRELVARYGRDLCSDARRCEGLLRDLCGEHRREINI
                     LVSALKERVPLDLLAAQSAMPRGLLLARLAKRLEDHLALTEEAARWAVDSWALALGVV
                     TDAEVAEREEKAEREKKAAPPPPPSTLANAPRPVPEEDADEAKSRATQSQTTRPAPPP
                     PQRRQQGAQPAARATTPAP"
     gene            2232..3008
                     /locus_tag="AC159P_03255"
     CDS             2232..3008
                     /locus_tag="AC159P_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015657011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HIR3694502.1"
                     /translation="MSRRPGGELASRPLHFIWIADSSGSMEQDGKIQALNTAIREAIP
                     HMKKVAEDNPNAQVLVRAVKFSNGAQWHISQPVPVADFAWTDLAAEGETDMGKALQLV
                     AEQLKMPPMSERALPPVLVLVSDGQPTDDFEAGLKALMNEPWGKKAVRIAISIGRDVD
                     AEVVQKFIGNSELKPLAANSPEALVRHIKWASTAVLKSASSPAADADMPGAGTTPIPI
                     PTAAPAADAQAAAAPGAQPPPAQTQADTDADNQKTIDDVW"
     gene            3019..4173
                     /locus_tag="AC159P_03260"
     CDS             3019..4173
                     /locus_tag="AC159P_03260"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF025053.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein
                     phosphatase"
                     /protein_id="HIR3694503.1"
                     /translation="MTETAAVPEWRVIGETVPGASHLRAGLPNQDAILQVRESGRGLP
                     LVVSVSDGHGSHKCFRSHRGSRFAVRRAAQLVGEFLDERRGAFDEEEIARALKETVPR
                     EFVRRWREAVEADLKARPFAAEELEALERKDGARARKLVEENPLLAYGATSLTVAVEE
                     SFILYLQLGDGEILCVSESGEVSKALAGDSRLFANETTSLCTADAEKNFRTRLQTLAA
                     ERPALVLVTTDGYANSFADEAGFLKVGSDLLEMLRADGIDPINASLKGWLEEATRAGS
                     GDDTTLAIVCRVDALKLPQTEARTKTAAAATAASETKAVAPETKAVVSETNDAPPQTA
                     TAEVMMKEPATTKDAAPPVSRNASASALANAVAPPIKPRGAVKPGERKSE"
     gene            4170..5765
                     /locus_tag="AC159P_03265"
     CDS             4170..5765
                     /locus_tag="AC159P_03265"
                     /inference="COORDINATES: protein motif:HMM:NF024627.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HIR3694504.1"
                     /translation="MNELVRQGLGEIVARHGPSVVEDRRRCEGLLRDHFGQYRREVSV
                     LSSALEERVPHDLLAAPAGVPREVLLARLARRLSDHLALAEDAARWSVNSWALALGVI
                     PDEELKHIERRHAPNATEPQGDATGKPVTAEAAALSSTAPQASAHTAADTAAQSAPPP
                     SVATSSVASSLVVSAHGGGDYTSINEALKHAAPGARLVVRPGVYEEGVTIDKEVEIVG
                     DGPVEQIVVTSDAASCLLMRAARARVAGLTLRGEAGRGGDGFFAVEIAGGRLLLEDCD
                     ISSRTLSCVAVHGAATEPLIRRCRVHDGADSGLYFFDGAAGTLEDCAVYANANVGVAV
                     TARANAIIRRTSIHDGGNAGLAVWGAGVVLLEECDIYGNRLAGVGVSDAGRLTARACR
                     IHEGDNTGVFVHRRGDATLEDCDIDGHRETEVAVEGRGQLTALRCRVRRSHGNGFFVR
                     DGGQASIQECEVTGNAGSGVSVAAGSLVAVLDCRVNANEGAGVKVAEGAAARVSDSDL
                     TGNRLGPWDAEEGAHLEGGGNVE"
     gene            5862..7184
                     /locus_tag="AC159P_03270"
     CDS             5862..7184
                     /locus_tag="AC159P_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002755455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR3694505.1"
                     /translation="MDQMLRPGQSVFCEPSGMSCVVETLLGAGGQGEVYKATLGGEAV
                     ALKWFFPHMGTAEQRAAIELLVRKGPPSEHFLWPLEVTTAAGVEGFGYVMPLRPARFK
                     NIVDLMKRRIEPTFRALATAGLNLSHNYLLLHSQGLCYRDISFGNAFFDPDGGDILIA
                     DNDNVSVDGAGLLGVLGTPRFMAPEVVRGEAVPSTQTDLFSLSVLIFYMLMVAHPLEG
                     KKETEIKCLDLPAMTKLYGTEPLFIFDPQDASNAPVPGIHDNALAFWRIYPQFLRDLF
                     TRAFTAGIRDPAGGRVRESEWRGAMVALRDTIFYCPHCALENFYDGAALRARGGRAGL
                     CWSCATELRLPPRVRIGRSVVMLNHDARLYPHHVDEKSMYDFSAPVAEVAAHPTSPGV
                     WGLRNLSATKWVIHTAEGAVKDVEPGRSVSLAVGTRIHFGKIEGEIRL"
     gene            7232..8647
                     /locus_tag="AC159P_03275"
     CDS             7232..8647
                     /locus_tag="AC159P_03275"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013892037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HIR3694506.1"
                     /translation="MYRTTFRQGGRGPRLALSGVALLFFVVAGGAGNEGTDARQQPPP
                     RANAGAVEQSGAASPQAKPQGVLTGKSALGDWTTDAPGVRRRITTADLPKPFETKSAN
                     NDARLVPRPEGAWPKAPAGFKVEEFATGLQNPRLLRVAPNGDIFIAESRANQIRVLRA
                     GEGKGKAESMEVFASGLSRPFGIAFYPSGPEPQWVYVANTGSVVRFPYRNGDTKARGA
                     AEVIVPDLPGGGQLRGGGHWTRDVVFSKDGTRMFVSVGSLTNVWEKPDDKEERRADIL
                     VYTPDGKNERVYAWGIRNAVGIAIHPQTGELWASVNERDGLGDDLVPDYITRVREGGF
                     YGWPWFYLGPNQDPRHQGKRPELRDKTIVPDVLIQSHSASLQMEFYTGAQFPAEYRHE
                     AFASQHGSWNRARRTGYKVIRVPQREGVPTGEYEDFLVGFVTPAGEVWGRPVGVAVVR
                     DGALFVTDDGSNTVWRVSYQK"
     gene            complement(8736..>9054)
                     /locus_tag="AC159P_03280"
     CDS             complement(8736..>9054)
                     /locus_tag="AC159P_03280"
                     /inference="COORDINATES: protein motif:HMM:NF012894.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HIR3694507.1"
                     /translation="AKISPSEVARLQEIRGLLIRATDTRVVIEGHTDNVGDTGTNLRL
                     SQERARSVWQWLKDSDPTGINISDRRLEGTEGYGPYRPLPGNQNRDEAEKAANRRVVI
                     ILK"
BASE COUNT         1487 a         3072 c         3147 g         1348 t
ORIGIN      
        1 ctcgggcttg ccccagagcg tgccctccca atcgctcgtc tgccgcagca ggtgttccca
       61 ggtgatcttg cggttgtgct ctgactcgaa aggctcgatg agctgcggct cgccgagcgc
      121 ttcggcgcgg cgcgcgccac ggttcggcgc gagcggatag acgggcgcca tgtagtcgcg
      181 cacgcggtct tgcaggctgc gaatcagctt gcggtcgtgg gcgaggccga ccgtggtgga
      241 gaggaaactt ttggtgacgc tgaaggtcat gtccacgcgc aaggggtcgc cccactcggc
      301 gacgacgtag ccgtggcgga tgacgacgcc ggtcgggtcg ccgcgcccct tgaaggggcc
      361 gaccgcgtgg ccgaaaggct cgcgcccgaa cgtctggtag tgggccagct caaggtcgcg
      421 cggcgccttc gattcggcgg cgacggcgaa ggcgacggcc tcctgaagtc cggcggcgtc
      481 catccccgcc tgctgcggcg tgcggcgctc ccactcttcc gcggggtaat aggcccgcgc
      541 cgcggacgcg ggcgtcgtgc gctgcgcgcg aagctgcaaa gaaaaagaag aagaagcggc
      601 gagcaggagg gccagcaaaa aaaccggcgc gccgcggcgt ctgctccgaa gtgctttcaa
      661 cgatgtgctc tcctcgattc gcgtgggtct ttcggcgaag gccgcacgtg ccggacggct
      721 cggacgccga cacgtgcgaa gcgcaattta tatcactctc gggcggcgga gggcagcatg
      781 tactgccgct gtatgtttcc gcgtcgggcc acggcgcagg cgtgcggtgc gcgcctgcgc
      841 gcgtgaatgg gcggcgcgct cggccgtcgg tcaagcgggc gtgcggtcgc ggacgtgggg
      901 gcgcgcgccg tcgcgtccgc acgcccgcac ggcggcagag gaacggtcgt tgccttacgt
      961 tgatggagta cccgagacag cgccgaaaca gtacgccccg gaggggtgaa ggagtgcgcg
     1021 cgtgagacgg tcgagtcgtt cgggtgaagg tggatgaagg ataaagtcat gagattcggt
     1081 tccctcgccg tctgttcgct cttcgtgtcg caaacgtcgt gcatagtggt cggcggctac
     1141 agcgacgacg gcgggtggtt tatctggccg tggggcctcg tcatctccgc ggtcttcatc
     1201 ggactgtttc tgctcctgag aaggcggcgc tagatgaagc gggcgaggta tgaatgacgg
     1261 gccgcgccag accttgcgcg aactcgtcgc gcggtacggg cgcgacctgt gcagcgacgc
     1321 gcggcgctgc gagggcttgc tccgcgacct gtgcggcgaa caccggcgcg agatcaacat
     1381 cctcgtcagc gccctgaagg agcgcgtgcc gttagacctg ctggcggcgc agagcgcgat
     1441 gccgcgcggg ctgctgctcg cgcgtctggc gaaacgtctc gaagatcatc tggctttgac
     1501 cgaagaggcc gcgcgttggg ccgtggattc gtgggcgctg gcgctcggcg tggtgactga
     1561 cgcggaggtc gcggaacgcg aggagaaagc cgagcgagag aagaaagccg caccgccgcc
     1621 gccgccttcc acattggcca acgcgccgcg gcccgtgcct gaagaggacg cggacgaggc
     1681 gaagagtcgc gcaactcaat cgcagacgac gcgacccgcg ccgccgcctc cgcagcgacg
     1741 gcaacaggga gcgcaaccgg cggcacgcgc gacgactccc gcgccgtagt catcgccgcc
     1801 gccgaccgtc gcacgaccgc cgagcagaca gtcgccgccg cccgcgccgt cgccgaagac
     1861 atcatggccg cccccgtcac cctggggccg catatggttt ccggtcagaa gacgatccac
     1921 ggcggcgcaa gcgagcacga agacggcttc ggggcgcgcg cttccctccc ccgcgccgac
     1981 gcaagaccct tcgacgcgcg ggcgaggccg gacgctgcgc ggctgtacgg tcggctgcct
     2041 gctgctcgtg ctcctgacgc tggcgctcgt cttcggcggg ccgttcgtcc tgtcggtgct
     2101 gcgcgaagaa caggagcgga ggaaccttga accgtcgccg gtgctgtcgc cttgagcggg
     2161 ttgcgcgggc ggaagacgtg ctttgattgt tcgattgact tttaaacgag aagagacaag
     2221 gagagacgac tatgagcaga cgaccgggcg gcgaactcgc ttcgaggccg cttcacttca
     2281 tctggatcgc cgatagctcc ggctcgatgg agcaggacgg gaagatacag gctttgaaca
     2341 ccgccatccg cgaggccatc ccgcacatga agaaggtggc cgaggacaac ccgaacgcgc
     2401 aagtgctggt gcgcgccgtg aagttctcga acggcgcgca gtggcacatc tcgcagcccg
     2461 tccccgtcgc cgacttcgcg tggacagacc tcgccgccga aggcgagacc gacatgggca
     2521 aggccctgca actcgttgcc gagcaactga agatgccgcc gatgagcgag cgagcgctgc
     2581 cgcccgtcct cgtcctcgtc tccgacggcc agccgaccga cgacttcgag gccggcctca
     2641 aagctttgat gaacgagccg tggggcaaga aggcggtgcg catcgccatc tccatcgggc
     2701 gcgacgtgga cgccgaagtc gtgcagaagt tcatcggcaa ctcggagctg aagccgctcg
     2761 ccgcgaacag ccccgaggcg ctcgtgcgcc acatcaagtg ggcctcgacc gcggtcttga
     2821 agtcggcctc gtcgcccgcc gccgacgccg acatgcccgg cgcggggacg acacccatcc
     2881 ccatcccgac cgccgcgccc gccgcggacg cgcaggccgc cgccgcgccc ggcgcacagc
     2941 cgccgcccgc gcagacgcag gccgacacgg acgccgacaa ccagaagacc atcgacgacg
     3001 tgtggtaagg agctgggcat gaccgagacc gcagccgtgc cggagtggcg cgtcatcggc
     3061 gagacggtgc ccggcgcgtc gcacctgcgc gccggactgc cgaatcagga cgccatcctg
     3121 caagtgcgcg agtcggggcg ggggctgccg ctcgtcgtct ccgtctccga cggccacggc
     3181 agccacaagt gtttccgcag ccaccgcggc tcgcgcttcg ccgtgcgcag ggccgcgcag
     3241 ctcgtcggcg aatttctcga cgagaggcgc ggcgctttcg acgaagagga gatcgcgcgc
     3301 gcgctcaagg agaccgtgcc gcgcgagttc gtccgccgct ggcgcgaggc ggtcgaggca
     3361 gacctcaagg cgcggccctt cgcggcggaa gaactcgaag cgcttgagcg gaaggacggc
     3421 gcgcgcgcgc gcaagctggt cgaagagaac ccgctgctcg cctacggcgc gacctctctg
     3481 accgtcgccg tcgaggagtc gttcatcctc tacctgcaac tcggcgacgg agagattctc
     3541 tgcgtctcgg agtcgggcga agtcagcaag gctctcgccg gagactcgcg cctcttcgcc
     3601 aacgagacga cctcgctctg cacggcggac gcggagaaga atttccgcac gcggctgcaa
     3661 acgctcgccg ccgagcggcc cgcgctcgtc ctcgtcacga ccgacggcta tgccaactcg
     3721 ttcgcagacg aagcgggctt cctcaaagtc gggagcgacc tactcgaaat gctccgcgcc
     3781 gacggcatcg accccatcaa cgcgagcctc aaaggctggc tcgaagaagc cacgcgcgcg
     3841 ggcagcggcg acgacaccac gctcgccatc gtctgccgcg tggacgcgct caagctgccg
     3901 cagaccgaag ctcgaaccaa gacggcggct gccgcgaccg ccgcgtccga gacgaaagcc
     3961 gtcgcgcccg agacgaaggc tgttgtgtcc gagacgaacg acgcgccgcc gcaaaccgcg
     4021 acggcggaag tcatgatgaa ggaacctgcg acgacgaagg acgccgcgcc gcccgtgtca
     4081 cgcaacgcgt ccgcgtcggc gcttgcgaac gccgtcgcgc cgccgatcaa gcctcgcgga
     4141 gccgtcaagc ccggcgagcg taagagcgaa tgaacgaact ggtgagacag gggctaggcg
     4201 agatcgtcgc gcggcacggc ccgtccgtcg tcgaagaccg gcggcgctgc gaaggactgc
     4261 tgcgagacca cttcggccaa taccgccgcg aggtctcggt gctctcttcg gcgctcgaag
     4321 agcgcgtgcc gcacgacctg ctcgccgcgc ccgcgggcgt gccgcgcgaa gtcctgctcg
     4381 cacgcctcgc acgccgcctc tcggatcacc tcgcgctcgc cgaagacgcc gcgcgctggt
     4441 ctgtcaattc gtgggcgctc gccctcggcg tcatccccga cgaagagctg aagcacatcg
     4501 aacgccgaca cgcgccgaac gccaccgagc cgcagggcga cgcgaccgga aaacccgtga
     4561 ccgccgaagc agcggcgctc tcgtcaaccg caccgcaagc atcggcgcac accgccgccg
     4621 acaccgccgc acagtccgcc ccgccccctt ccgtcgccac ttcttccgtc gcgtcttcac
     4681 tcgtcgtctc ggcgcacggc ggcggcgatt acacgagcat caacgaagcc ctcaagcacg
     4741 ccgcgcccgg cgcgcgtctc gtcgttcgcc ccggcgtgta cgaagagggc gtcaccatcg
     4801 ataaagaggt ggagatagtc ggcgacggcc cggtcgaaca gatcgtcgtc acgagcgacg
     4861 ccgcgagctg cctgctgatg cgcgccgcga gggcgcgcgt ggcggggctg acgctgcgcg
     4921 gcgaggcggg ccgaggcggc gacggcttct tcgccgtcga gatcgcgggc ggcaggctcc
     4981 tgctcgaaga ctgcgacatc tcttcgcgca cgctctcgtg cgtggccgtc cacggcgccg
     5041 cgaccgagcc gctgattcgt cgctgccgcg tccacgacgg cgccgactcc ggtctctact
     5101 tcttcgacgg cgcggcgggc acgctcgaag actgcgcggt ctacgccaac gccaacgtcg
     5161 gcgtcgccgt caccgcgcgc gcgaacgcaa tcatcaggcg caccagcatc cacgacggcg
     5221 gcaacgcggg gctggccgtg tggggcgcgg gcgtggtgct cctcgaagag tgcgacatct
     5281 acggcaaccg cctcgcgggc gtcggcgtga gcgacgcggg gaggctgacg gcgcgcgcgt
     5341 gtcgcatcca cgagggcgac aacacaggcg tcttcgtcca ccgccgcggc gacgccacgc
     5401 tcgaagactg cgacatcgac gggcaccgcg aaacggaggt cgccgtcgag ggccgcggcc
     5461 aactgaccgc cctccgctgc cgcgtccgac ggagccacgg caacggcttc ttcgtccgcg
     5521 acggcgggca ggcttcgatt caggagtgcg aggtgacggg taacgccggc agcggcgtct
     5581 cggtcgccgc aggctcgctc gttgccgtcc tcgattgccg cgtcaacgcg aacgagggcg
     5641 cgggcgtgaa ggtcgccgag ggcgcggcgg cccgcgtctc cgacagcgac ctcacgggca
     5701 accgcctcgg cccgtgggat gcggaagagg gcgcgcacct cgaaggcggc ggcaacgtgg
     5761 aatagaagaa agtcggaagt cggaacgatg aaggatgaat aaaaagcatt ttgtcttcat
     5821 tcatcgttcc gacttctgac ttctgacttc ggggggttgt gatggatcag atgttgaggc
     5881 cgggtcagtc ggtcttttgc gagccgtcgg ggatgtcgtg cgtggtggag accttgctcg
     5941 gcgcgggcgg gcagggcgag gtttacaagg cgacgctcgg cggcgaggcg gtggcgctga
     6001 agtggttctt cccgcacatg gggacggcgg agcagcgcgc ggccatcgag cttcttgtgc
     6061 ggaaggggcc gccgagcgag cacttcctgt ggccgctgga agtgacgacc gccgcgggcg
     6121 tcgagggctt cgggtacgtg atgccgctcc ggcccgctcg cttcaagaac atcgtcgatt
     6181 tgatgaagcg gcgcatcgag ccgacgttcc gcgcgctcgc caccgcgggc ctcaacctct
     6241 cgcacaatta cctgctgctc cactcgcagg gcctctgcta ccgcgacatc tctttcggca
     6301 acgccttctt cgacccggac ggcggcgaca tcctgatcgc cgacaacgac aacgtctcgg
     6361 tggacggcgc ggggctgctg ggcgtcttgg gcacgccgcg cttcatggcg cccgaggtgg
     6421 tgcgcggcga ggccgtccct tccacgcaga ccgacctgtt ctcgctctcc gtgctcatct
     6481 tctacatgct gatggtcgcg cacccgctcg aaggtaaaaa ggagacggag atcaagtgcc
     6541 tcgaccttcc cgccatgacg aagctctacg gcacggaacc gctcttcatc ttcgacccgc
     6601 aggatgcgtc gaacgcgccc gtccccggca tccacgataa cgcgctcgcc ttctggcgca
     6661 tctacccgca gttcctgcgc gacctcttca cgcgcgcgtt caccgccggg atacgcgacc
     6721 cggcgggcgg gcgcgtgcgc gagagcgagt ggcgcggcgc gatggtcgct ttgcgcgaca
     6781 ccatcttcta ctgcccgcac tgcgcgctcg aaaatttcta cgacggcgcg gcgctccggg
     6841 cgcgcggcgg gcgggcgggc ctctgctggt cgtgcgcgac ggagctgcgg ctgccgccgc
     6901 gcgtgcgcat cggcaggtcg gtcgtcatgc tcaaccacga cgcgcggctc tacccgcacc
     6961 atgtcgatga aaagtcgatg tacgacttct cggcgcccgt cgccgaggtg gccgcacacc
     7021 cgaccagccc cggcgtgtgg ggactcagaa atctgtccgc gacgaagtgg gtcattcaca
     7081 cggcggaagg cgcggtcaag gacgtggagc cgggacgcag cgtctcgctc gcggtcggca
     7141 cgcgcatcca cttcggcaag atcgaaggcg agatcagatt gtgagcggcg gcggttggcc
     7201 gttatcaaga acgaagggtt atgatcgacg tatgtacagg acaactttcc ggcagggtgg
     7261 gcgcgggccg agactcgcgc tctcgggcgt cgcgcttctt ttcttcgtcg tggcgggcgg
     7321 cgccggcaac gagggcaccg acgcgcggca gcagccgccg ccccgcgcga acgccggcgc
     7381 ggtcgaacag tccggcgcgg ccagtccgca ggcgaagccg cagggcgtgc tgacgggcaa
     7441 gtccgcgctc ggcgactgga cgacggacgc gcccggcgtg cgccgacgca tcacgacggc
     7501 agacctgccg aagcctttcg agacgaagtc cgcgaacaac gacgcgcggc tcgtgccgcg
     7561 ccccgaaggc gcgtggccga aggcgcccgc gggcttcaag gtcgaagagt tcgcaacggg
     7621 tcttcagaac ccgcgtcttc ttcgcgtcgc gccgaacggc gacatcttca tcgccgagag
     7681 ccgcgccaat caaatccgcg tcctgcgcgc gggcgaaggg aaaggcaagg ccgagtcgat
     7741 ggaggtcttc gcgtcgggac tgagccgccc gttcggcatc gcgttctacc cgtcggggcc
     7801 ggagccgcag tgggtctacg tcgccaacac cggctcggtc gtccgcttcc cttaccgcaa
     7861 cggcgacacg aaggcgcgcg gggccgctga agttatcgtg cccgaccttc cgggcggcgg
     7921 ccagcttcgc ggcggcggcc actggacgcg cgacgtggtc ttctcgaaag acggcacgcg
     7981 gatgttcgtc tcggtcggct cgctgacgaa cgtctgggag aagcccgacg acaaggagga
     8041 gcgccgcgcg gacatcctcg tctacacgcc cgacggcaag aacgagcgcg tctacgcgtg
     8101 gggcatccgc aacgccgtcg gtatcgcgat acatccgcag acgggcgagt tgtgggcctc
     8161 cgtcaacgag cgcgacggcc tcggcgacga cctcgtgccc gactacatca cgcgcgtccg
     8221 cgagggcggc ttctatggct ggccgtggtt ctacctcggc cccaaccaag acccgcgcca
     8281 tcaaggcaag cgccccgagc tgcgcgacaa gaccatcgtg cccgacgtgc tcatccagtc
     8341 gcactcggcc tcgctccaga tggagttcta cacgggcgcg cagttccccg ccgagtaccg
     8401 ccacgaagcc ttcgcctcgc agcacggctc gtggaaccgc gcgcggcgca ccggctacaa
     8461 ggtgatccgc gtgccgcagc gcgaaggcgt gccgacgggc gagtacgaag atttcctcgt
     8521 cggcttcgtc acgcccgcgg gcgaagtatg gggccgtccc gtcggcgtcg cggtggtgcg
     8581 cgacggcgcg ctgttcgtca cggacgacgg ctcgaacacg gtgtggcgcg tctcgtatca
     8641 aaaatagaag tactgagtac tgagtgctga gtgctgagtg aaagcattgt ctttcactca
     8701 gtactcagca ctcagtactc agtactttag tggagttact tcaggataat cacgacgcgg
     8761 cggttcgcgg ccttttcggc ctcgtcgcgg ttctggttgc cggggagggg ccggtagggg
     8821 ccgtagcctt cggtgccttc gaggcgccgg tcgctgatgt tgatgcccgt cgggtcggag
     8881 tccttgagcc actgccagac ggagcgcgcg cgctcctgcg agagccgcag gttggtgccc
     8941 gtgtcgccga cgttgtcggt gtggccctcg atgacgaccc gcgtgtcggt ggcgcgaatc
     9001 agcaggccgc gaatctcctg aaggcgcgcg acctccgagg ggctgatctt cgcc
//
LOCUS       DBNGOH010000176         2586 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4974684, whole genome shotgun sequence.
ACCESSION   DBNGOH010000176 DBNGOH010000000
VERSION     DBNGOH010000176.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2586)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2586)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2586
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4974684"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2586)
                     /locus_tag="AC159P_03285"
     CDS             complement(<1..>2586)
                     /locus_tag="AC159P_03285"
                     /inference="COORDINATES: protein motif:HMM:NF051698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HIR3694508.1"
                     /translation="SVENLPVLGRKFEDLLTLTPGVSVVQGPDGDEINFVGQRGVFNN
                     ISLDGGDYNNGFFGEQAGGQRAAIDIPLDAVQEFQVVATGATAEFGRTAGGVVNVITK
                     SGTNDFHGSLFHFQRLEALTANTSDGKELTDFHREQSGGTIGGPIRREKAFFFFALEH
                     VRDNLTRPNLSEQVGDTPCSVQTPTIGANEALINANADCQRLALLNFFRTTRNQEEGL
                     PIERPVRNTAFLGKLDWTLTPSNKLALSYNFDHSKNTNQTFDVETYGTSANGTEGPSK
                     IQVLNFNLFSAVTPTMVNEAHFTYGRESRPRSATDSNIPADTAMGFATTFRFGNPFFL
                     QPGVDELFWRTHLRDTFSIVKGGHTVKFGGEWLHSLNSQVFRGFFTGRYIFDSVTGFL
                     RYASPAAAGGFGPSTVGCSNGTFVTAPATCPAGTTATGGPLLLYLQGAGRTGPATDAA
                     GFSEISNEDLALFAQDSWKVRPNFTLNYGLRWEAQIFPDPVVPPAETAYGIFLSDPRF
                     PSDGTLPNQKKMFQPRVGFAWDLGSSGKSVLRASYGIYFARQNMLSQVGSITTNGVQQ
                     QTIFLNTPIISSGVPGPVWPGLVTPPTAGSCTAGAITNPFPCFSGVRVFSRDYANPRI
                     YTTNVAFEQELISDLSLYLDFTHAKGVHLTRFLNFGRTGFFAPFLDEVMVASAVGKSV
                     YRGFTVGMRKRYSRGFQFEWNYVLSKDEDDDSNERDPFTDRAFDFNNLQLDYALSDRD
                     IRHRFNFYTNAELPWGFEGNARVQARTAQPITPACPTAPRCPNRNTLRKNNEFFSFDW
                     RLQRPFGIGESFRIIPIIEMFNTFNNSNNVNPLVAPALFNFDGFLRQGVGDPRQVQLA
                     IKAEF"
BASE COUNT          411 a          729 c         1003 g          443 t
ORIGIN      
        1 aaaactccgc cttgatggcc agctgaacct gacgcgggtc gccgacgccc tgccggagga
       61 agccgtcgaa gttgaagagc gccggcgcga ccagcgggtt gacgttgttc gagttgttga
      121 aggtattgaa catctcgatg atcgggatga tgcggaagct ctcgccgatg ccgaagggcc
      181 gctgcaagcg ccagtcgaac gagaagaact cgttgttctt gcgcagggtg ttgcggttgg
      241 ggcagcgcgg ggccgtgggg caggccggcg tgatgggctg ggcggtgcgc gcctgcacgc
      301 gcgcgttgcc ctcgaagccc cacggcagct cggcgttggt gtagaagttg aagcggtggc
      361 ggatgtcgcg gtccgacaga gcgtagtcga gctggaggtt gttgaagtcg aaggcgcggt
      421 cggtgaacgg gtcgcgctcg ttcgagtcgt cgtcctcgtc cttcgacaaa acgtagttcc
      481 actcgaactg gaagccgcgg ctgtagcgct tgcgcatccc gacggtgaag ccgcggtaga
      541 cggacttgcc gacggcgctc gccaccatca cctcgtcgag gaacggggcg aagaagcccg
      601 tgcgaccgaa gttgaggaag cgcgtcaggt ggacgccttt ggcgtgcgtg aagtcgaggt
      661 agagcgagag gtcggaaatc aactcctgct cgaaggcgac gttggtggtg tagatgcgcg
      721 ggttggcgta gtcgcggctg aagacgcgca cccccgagaa gcaggggaac gggttggtga
      781 tggcgcccgc ggtgcaggag cctgcggtcg gcggcgtcac cagtccgggc cagacggggc
      841 cgggaacgcc cgacgagatg atcggcgtgt tcaggaagat ggtctgctgc tggacgccgt
      901 tggtcgtgat cgagccgacc tgcgagagca tgttctggcg cgcgaagtag atgccgtagc
      961 tggcgcgcag gacggacttg ccgctgctgc cgaggtccca cgcgaagccg acgcgcggct
     1021 ggaacatctt cttctggttg gggagcgtgc cgtcggaagg gaagcgcggg tcgctgagga
     1081 agatgccgta ggcggtctcg gcgggcggca cgaccgggtc ggggaagatt tgcgcctccc
     1141 agcgcaggcc gtagttgagc gtgaagttgg gccgcacctt ccaggagtcc tgcgcgaaga
     1201 gcgcgaggtc ttcgttgctg atctcggaga agcccgcggc gtcggtcgcc gggccggtgc
     1261 gccccgcgcc ttgcaggtag aggaggagcg ggccgccggt cgccgtcgtg ccggcggggc
     1321 aggtcgcggg ggccgtgacg aacgtgccgt tcgagcagcc gacggtgctc ggcccgaagc
     1381 cccctgcggc ggcgggcgag gcgtagcgca ggaagcccgt caccgagtcg aagatgtagc
     1441 gcccggtgaa gaagccgcgg aagacctgcg agttgaggct gtgcagccac tcgccgccga
     1501 acttgacggt gtgcccgcct ttgacgatgg agaaggtgtc gcgcaggtgc gtgcgccaga
     1561 acagctcatc gacgccgggc tggaggaaga acgggttgcc gaagcggaag gtggtggcga
     1621 agcccatcgc cgtgtcggcg gggatgttcg agtcggtggc ggagcgcggc ctcgattcgc
     1681 gcccgtaggt gaagtgcgcc tcgttgacca tcgtcggcgt gacggcgctg aagaggttga
     1741 agttcaggac ctgaatcttc gaaggccctt cggtgccgtt ggcagaggtg ccgtaggtct
     1801 ccacgtcgaa ggtctggttg gtgttcttcg agtggtcgaa gttgtaagag agcgcgagct
     1861 tgttcgaggg cgtcagcgtc cagtcgagct tgccgaggaa ggcggtgttg cgcaccgggc
     1921 gctcgatggg caggccctcc tcctgattgc gcgtggtgcg gaagaagttg aggagcgcga
     1981 gccgctggca gtccgcgttc gcgttgatga gcgcctcgtt ggcgccgatg gtcggcgtct
     2041 gcacggagca gggcgtgtcg ccgacctgct cggagaggtt cgggcgcgtc aggttgtcgc
     2101 ggacgtgctc aagggcgaag aagaagaacg ccttctccct gcggatcggc ccgccgatgg
     2161 tgccgcccga ctgctcgcgg tggaagtccg tcagctcctt gccgtccgag gtgttcgccg
     2221 tcagcgcttc gaggcgctgg aagtggaaga gcgagccgtg gaagtcgttg gtgcccgact
     2281 tggtgatgac gttgacgacg ccgccggcgg tgcgcccgaa ctcggcggtc gcgcccgtgg
     2341 cgacgacctg gaactcctgc acggcgtcga gcgggatgtc gatggcggcg cgctggccgc
     2401 cggcctgctc gccgaagaag ccgttgttgt agtcgccgcc gtcgaggctg atgttgttga
     2461 agacgccgcg ctggccgacg aagttgatct cgtcgccgtc cggcccctgc acgacggaga
     2521 cgccgggcgt cagggtcagc aggtcttcga acttcctgcc gagcaccggc aggttctcga
     2581 ccgagc
//
LOCUS       DBNGOH010000177         6758 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4791499, whole genome shotgun sequence.
ACCESSION   DBNGOH010000177 DBNGOH010000000
VERSION     DBNGOH010000177.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6758)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6758)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6758
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4791499"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(88..2493)
                     /locus_tag="AC159P_03290"
     CDS             complement(88..2493)
                     /locus_tag="AC159P_03290"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GumC family protein"
                     /protein_id="HIR3694509.1"
                     /translation="MSQDNRLLPLSSANESLDRPLRDVAHSAPVASASGAEPAHLREY
                     LAVVLKRKWLILSLMVVVTSLVAIQMYRLPSIYSARTQIQIEQKQRSVLQTSRTGDLI
                     IRGGNDPAYWNTQIKKLESQQLARQVVTRLDLQNNPAFLGANRGSGLITSLRRIVSRE
                     KAAPAQAAADEAGVPVLNENVATTIESLTPEQARQLEPYEDKLREGLTIEPIERTNLV
                     YIHFRHNEPQIAMNVANTVAQIFRENDINTESSGADKALVTLGRQITELQEQIRADEA
                     ARIAYMGKHDIPLSKNQAADLVAVQLGTYSSQMQQAEADRKDKEVAYRAAAAAMERGD
                     VLSIPSVQENGAVKRIREKIEGLQSQREALLVQYTAEWPAVKQIDNQITQLEAEMNRI
                     PSQVVGMMKTSYEQSVNKENQLKRSYSTKRGEATQQTKAEIGLSDLNQRIETNKQLYQ
                     TYLARQRELQVTNSDRTNNISQVEAARLPREPIGPPRVRNIMIALLLALAVGVGLAFL
                     LDYLDDTLKSVEDVDRHLHLPTLALIPAPREARRMLGRAAPSPAPGDPSTALALIVDV
                     RSPVAEAYRHLRTSLLLSSAGQPPKTILVTSSQPSEGKTTTVVNIAMMLAQTGAEVLV
                     LDCDLRRPRVHSHFGIGNARGVTNYLSGDAQASEIIQTSERLPANLKIIPSGPIPPNA
                     AELLGSDEMRRLLYVLSENFTHIVIDSPPAISFTDASILSTMVDGVMLVVHGGRSSRA
                     VVRRAKQQLQDVGAHLFGIVLNNVKLEANDYYYYTGYYSGYYAADDEGTPEGAETAGN
                     GRG"
     gene            complement(2766..3617)
                     /gene="rfbD"
                     /locus_tag="AC159P_03295"
     CDS             complement(2766..3617)
                     /gene="rfbD"
                     /locus_tag="AC159P_03295"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:NF016228.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HIR3694510.1"
                     /translation="MKVLITGAGGMVGRALAEHCRGRGDEVAAHERGSLDIADERAVG
                     EALARARPAAVFNCAAWTDVDGCELDPQRAFQVNARAVEILATQSRLAGASFVTLSTD
                     YVFDGSKEGFYTQRDDPHPASVYGAAKLEGERRAQLASARTSVVRTGWVFGSGGRNFL
                     ATVVERARRGETLKAIADAYGTPTYAPDLAARLRELAELDLPGVYHVVNAGDGTSFEG
                     FAREAVRAAGADGAPVEPVSMNSLSRPAPRPRNSRLRCLVSEAIGLAPLRDWRDALAD
                     FASASRQ"
     gene            complement(3753..4766)
                     /gene="rfbB"
                     /locus_tag="AC159P_03300"
     CDS             complement(3753..4766)
                     /gene="rfbB"
                     /locus_tag="AC159P_03300"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010877391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HIR3694511.1"
                     /translation="MDKLFITGGAGFIGSAFVRLVLAEHPTARVTNFDALTYAGNLDN
                     LEGLDEARHRFVRGDICDREAVLAALEEGTDALVNFAAESHVDRSITSAGEFVRTNVL
                     GTQALLDAARARGVKRFVQVSTDEVMGSLPEDDTAFFTEASPFEPNSPYAASKAAAEH
                     FVRAANHTHKLDTVVTRCGNNYGPRQFPEKFLPLMLSNAMRDEPIPLYGDGRYVRDWI
                     HVEDHCRAVLAVMQRGRTGEAYNIGARNERRNIEVAESVLTALGKPLTLIRHVKDRPG
                     HDRRYAIDPTKIETELGWRPQETWESGLQKTIRWYADNPNWVARARSGEYREYYARQY
                     GEI"
     gene            4996..5982
                     /locus_tag="AC159P_03305"
     CDS             4996..5982
                     /locus_tag="AC159P_03305"
                     /EC_number="5.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015187750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HIR3694512.1"
                     /translation="MSSIEFNDCAARVVKLLRLEAEAVRLCAERLDAAEVARAVELLA
                     ACRGKVVLVGVGKSGIIARKIAATLTSTGTVAVYLHPSDGLHGDLGIVTEGDVAVVLS
                     NSGETDELTAMLPYLKRRSVPLVAIVGNPGSTLARKADAVIDARVDREMCPFNLAPTT
                     STTVALAVGDALAITLMQLKGLTPDDFALNHPAGRLGKRLSLRVADLMHGGPDNPTVA
                     PTATWLEVVNAISRGGLGAVSVVGGGRLVGLITDGDLRRAVARTDPRELPNLVAEAFM
                     TRDPVTVGTEELAYDALQLMERRASQISVLPVTQGDACVGMLRLHDIVRSGL"
     gene            6035..6580
                     /gene="rfbC"
                     /locus_tag="AC159P_03310"
     CDS             6035..6580
                     /gene="rfbC"
                     /locus_tag="AC159P_03310"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HIR3694513.1"
                     /translation="MTTIKETKLRGAFIIEPHRHEDERGFFARAWSEGELAERGVGPL
                     VEANISYNSRKWTLRGMHYQAAPHGQGKLLRCTRGAIYDVGVDLRPESPTFMQWVGVE
                     LTEQNRLLLYLPPEFAHGFQTLRDDTEVFYMVSSLYTPESYRGVRWDDPAFGIEWPHA
                     DRRIINERDRTYPDFTPPSKL"
BASE COUNT          999 a         2230 c         2406 g         1123 t
ORIGIN      
        1 ggcggacaga aagaaggcgg aggggtgaaa gcggaagcct ggcggctgcg ctttcacccc
       61 tccgccgtca tcgtccgtcc ctcgccttca gccgcgaccg ttgcccgccg tctcggcgcc
      121 ttcgggcgtg ccctcgtcgt cggcggcgta gtagcccgag tagtagccgg tgtaatagta
      181 gtagtcgttg gcttcgagct tgacgttgtt gagcactatg ccgaagaggt gcgcgcccac
      241 gtcctggagc tgctgcttgg cgcggcgcac gaccgcgcgc gaagagcgac cgccgtggac
      301 gacgagcatc acgccgtcca ccatcgtcga gaggatcgag gcgtcggtga acgagatggc
      361 gggcggcgag tcgatgacga tgtgcgtgaa gttctccgag agcacgtaga ggaggcggcg
      421 catctcgtcc gagccgagca gctccgccgc gttcggcggg atcggccccg aggggatgat
      481 cttcaggttg gcgggcaggc gctcgctggt ctggatgatc tcgctcgcct gcgcgtcgcc
      541 cgacaggtag ttggtcacgc cgcgcgcgtt gccgatgccg aagtgcgagt ggacgcgcgg
      601 gcgcctgagg tcgcagtcga ggacgagcac ctcggcgccg gtctgcgcga gcatcatggc
      661 gatgttgacg accgtcgtcg tcttcccttc cgagggctgg ctcgaagtga cgaggatggt
      721 cttcggcggc tggcccgccg aggagagcag caaagaggtg cgcaggtggc ggtacgcctc
      781 ggcgacgggc gagcgcacgt ccacgatgag cgcgagcgcg gtcgaggggt cgccgggcgc
      841 gggcgacggg gcggcgcgac cgagcatgcg gcgggcctcg cgcggcgcgg ggatgagcgc
      901 cagcgtcggc aggtgcaggt ggcgatccac gtcctcgacc gacttcagcg tgtcgtcgag
      961 gtagtccagg aggaaggcga ggccgacgcc gacggcgagc gcgagcagca gcgcgatcat
     1021 gatgttgcgc acgcgcggcg ggccgatggg ttcgcgcggc aggcgcgccg cctcgacctg
     1081 cgagatgttg ttggtgcggt cgctattggt gacttgcagc tcgcgctggc gtgcgaggta
     1141 ggtctggtag agctgcttgt tggtctcgat gcgctggttg aggtcgctga ggccgatctc
     1201 ggccttcgtc tgctgcgtgg cctcgccgcg cttggtcgag tacgagcgct tgagctggtt
     1261 ctccttgttg accgactgct cgtagctggt cttcatcatc ccgacgacct gcgacgggat
     1321 gcggttcatc tcggcttcga gctgcgtgat ctggttgtcg atctgcttga cggcgggcca
     1381 ctccgccgtg tactgcacga gcagcgcctc gcgctgcgac tgcaagccct cgatcttctc
     1441 ccgaatccgc ttgaccgcgc cgttctcctg caccgagggg atcgacagca cgtcgccgcg
     1501 ctccatggcc gccgccgccg cgcggtaggc cacctccttg tccttgcggt cggcctcggc
     1561 ctgctgcatc tgcgagctgt aggtgccgag ctggacggcg acgaggtctg ccgcctgatt
     1621 cttcgagagc gggatgtcgt gcttgcccat gtaggcgatg cgcgcggcct cgtcggcgcg
     1681 aatctgctct tgcagctccg tgatctggcg accgagcgtg accagcgcct tgtccgcgcc
     1741 ggacgactcg gtgttgatgt cgttctcgcg gaagatctgc gccaccgtgt tggcgacgtt
     1801 catggcgatc tgcggctcgt tgtgccggaa gtggatgtag acgaggttgg tgcgctcgat
     1861 cggctcgatg gtgaggccct cgcggagctt gtcctcgtag ggttcgagct ggcgcgcctg
     1921 ctcgggcgtc agcgactcga tggtcgtcgc cacgttctcg ttgagcacgg gcacgcccgc
     1981 ttcgtccgcc gcggcttgcg cgggcgccgc cttctcgcgc gagacgatgc ggcgcagcga
     2041 ggtgatgagg ccgctgccgc ggttggcgcc gaggaaggcc gggttgttct gaaggtcgag
     2101 tcgcgtcacg acctggcggg cgagctgctg cgattcgagc ttcttgatct gcgtgttcca
     2161 gtaggccggg tcgttgccgc cgcggatgat gaggtcgccg gtgcggctcg tctggaggac
     2221 gctccgctgc ttctgctcga tctgaatctg ggtgcgcgcg ctgtagatcg agggcagccg
     2281 gtacatctgg atcgccacga gcgaggtgac gacgaccatc aggctcagga tgagccactt
     2341 gcgcttgagc acgacggcga ggtactcgcg caggtgcgcc ggctcggcgc ccgacgccga
     2401 ggcgacgggg gccgagtgcg ccacgtcgcg cagcgggcgg tcgagcgact cgttggcgga
     2461 ggagagcggc agcaggcgat tatcttgcga catcatgatt cttacttctc gattccgtgc
     2521 ttcttctgga actctctttg aagggacgac gcgtccgcgg gcgttattcg gcggggccta
     2581 tcataaccac gcccgcccgc cggggcaagt aggcaatcgg cagaaagcag taggcagtag
     2641 gcagcaggca acaggcagtc aaggcggaag acaggagtcg gaagaaggaa gggcgaagag
     2701 gcttggagct ttacctcgca agcctcttcg cccttcttct gtcttgactg cgtgctgcct
     2761 gctgcctatt gcctacttgc tgaggcgaag tcggcgagcg cgtcgcgcca gtcgcgcaag
     2821 ggggcgaggc cgatggcttc ggagacgagg cagcgcaggc gcgagttgcg cgggcgcggc
     2881 gcggggcgcg agagcgagtt catcgagacc ggctcgacgg gggcgccgtc ggcgcccgcc
     2941 gcccggacgg cctcgcgcgc gaagccctcg aagctcgtcc cgtcgcccgc gttgacgacg
     3001 tggtagacgc cgggcaagtc cagctcggcc agctcgcgca ggcgggccgc gaggtcgggc
     3061 gcgtaggtcg gcgtgccgta ggcgtccgcg atggccttca gggtttcgcc gcgccgcgcg
     3121 cgctcgacga ccgtggcgag gaagttgcgc ccgccgctgc cgaagaccca gcccgtgcgg
     3181 acgaccgagg tgcgcgccga ggcgagctgc gcgcgccgct cgccttcgag cttcgccgcg
     3241 ccgtagacgc tcgccgggtg cgggtcgtcg cgctgcgtgt agaagccctc cttcgagccg
     3301 tcgaagacgt agtcggtcga gagggtgacg aacgacgcgc ccgccagacg gctctgcgtc
     3361 gccagtatct cgacggcgcg cgcgttcacc tggaaggcgc gctgcgggtc aagctcgcac
     3421 ccgtccacgt ccgtccacgc cgcgcagttg aagaccgccg cgggccgcgc gcgcgccagc
     3481 gcctcgccga ccgcgcgctc gtccgcgatg tccagcgacc cgcgctcgtg cgccgcgacc
     3541 tcgtcgcccc gcccgcggca atgctccgcc agcgcacgcc cgaccatccc gcccgcgcct
     3601 gtgatgagaa ctttcataaa aacggtaggc agtaggcagc aggcagtagg cagaaaggaa
     3661 gacaaagagc ttttgctgcg agattcgaaa ttcgagattc cgaatccgaa accgctttga
     3721 ctgcctactg cctgctgcct actgcctgct tttcaaatct ccccgtactg ccgcgcgtaa
     3781 tattcgcggt actcgccgct gcgggcgcgg gcgacccagt tcgggttgtc cgcgtaccag
     3841 cggatggtct tttgaaggcc gctctcccag gtctcctgcg ggcgccagcc gagttcggtc
     3901 tcgatcttcg tcgggtcgat ggcgtagcgg cggtcgtggc ccggtcggtc cttgacgtga
     3961 cggatcaaag tgagcggctt gccgagcgcc gtcagaaccg actcggcgac ctcgatgttg
     4021 cggcgctcgt tgcgcgcgcc gatgttgtac gcctcgccgg tgcgcccgcg ctgcatgacg
     4081 gcgaggacgg cgcggcagtg gtcttcgacg tgtatccagt cgcgcacgta gcgcccgtcg
     4141 ccgtagagcg ggatcggctc gtcgcgcatc gcgttcgaga gcatgagcgg caggaacttc
     4201 tcggggaact ggcgcgggcc gtagttgttg ccgcagcgcg tgaccacggt gtcgagcttg
     4261 tgcgtgtgat tggcggcgcg gacgaagtgc tcggcggcgg ccttcgacgc ggcgtagggg
     4321 ctgttcggct cgaacggcga cgcttcggtg aagaaggccg tgtcgtcttc gggcaggctc
     4381 cccatcacct cgtccgtcga gacctgcacg aagcgcttga cgccgcgcgc gcgggcggcg
     4441 tccagcagcg cctgcgtgcc gaggacgttc gtgcggacga actcgcccgc cgaggtgatg
     4501 ctgcggtcaa cgtgcgactc ggcggcgaag ttgaccaagg cgtccgtgcc ctcttcgagc
     4561 gcggcgagca ccgcctcgcg gtcgcagatg tcgccgcgca cgaagcggtg gcgcgcctcg
     4621 tcgagtcctt cgaggttgtc gaggttgccc gcgtaggtga gcgcgtcgaa gttggtgacg
     4681 cgcgccgtcg ggtgttcggc gagcacgagc ctgacgaagg ccgagccgat gaagcccgcg
     4741 ccgccggtga tgaaaagttt gtccataaga gaaacggtaa aaggtcggaa gtaagtgcgg
     4801 gagtatagaa acgggcatcc ggggtgtcaa gaaagcccgc ccgccgcccg cctcatccgg
     4861 cgcgccacgc agggggcgcg gacgtgcggg gcgactaagg cgaagctggc gtttataatg
     4921 ccgcccggcg tatgctccac agtctcgccg ccgcggaaga gccggaggcg gtcgggggag
     4981 cgaagcagca gaagcatgtc gtccatcgaa ttcaacgatt gcgccgcgcg ggtcgtcaag
     5041 ctgctgcggc ttgaggccga ggccgtcagg ctgtgcgccg agcgcctcga cgcggctgag
     5101 gtcgcgcggg ccgtcgagtt gttggccgcc tgccgcggca aggtcgtcct cgtcggcgtc
     5161 ggcaagtcgg gcatcatcgc gcgcaagatc gcggcgacgt tgacgagcac cggcaccgtc
     5221 gccgtctacc tccacccgtc ggacggcctg cacggcgacc tcggcatcgt caccgaaggc
     5281 gacgtggccg tcgtcctcag caacagcggc gagaccgacg agctgacggc gatgctcccg
     5341 tacctgaagc ggcggagcgt cccgctcgtc gccatcgtcg gcaaccccgg ctcgacgctg
     5401 gcgcgcaagg ccgacgccgt gatcgacgcc cgcgtggaca gggagatgtg cccgttcaac
     5461 ctcgcgccga ccacgagcac gacggtcgcg ctcgccgtcg gcgacgcgct cgccatcacg
     5521 ctgatgcagc tcaaggggct gacgcccgac gacttcgcgc tcaaccaccc ggcgggccgg
     5581 ctcggcaagc gcctcagcct gcgcgtcgcc gacctgatgc acggcgggcc ggacaacccg
     5641 accgtcgcgc ccacggccac gtggctcgaa gtcgtcaacg ccatctcgcg gggcggactc
     5701 ggcgccgtca gcgtcgtcgg tgggggccgc ctcgtcgggc ttatcacgga cggcgacctg
     5761 cggcgggccg tcgcgaggac cgacccgcgc gagctgccga acctcgtcgc cgaagccttc
     5821 atgacgcgcg accccgtcac ggtcgggacg gaagaactcg cctacgacgc cctgcaactg
     5881 atggagcggc gcgcgtcgca aatctccgtg ctgccggtca cgcagggcga cgcctgcgtc
     5941 ggcatgctgc ggctccacga catcgtgcgc agcggccttt agagcgccgg ttgcgggcgg
     6001 cgcgcagggc tgtgctatca tccccgccgc cgttatgacc accatcaaag agaccaaact
     6061 gcgcggcgcc ttcatcatcg agccgcaccg ccacgaagac gagcgcggct tcttcgcgcg
     6121 ggcctggtcg gagggcgagc tggccgagcg gggcgtcggt ccgctcgtcg aggccaacat
     6181 ctcttacaac agccgcaagt ggacgctgcg cgggatgcac tatcaggccg caccgcacgg
     6241 ccagggcaag ctgctgcgct gcacgcgcgg cgccatctac gacgtgggcg tcgatctgcg
     6301 gcccgagtcg ccgaccttca tgcagtgggt cggcgtcgag ctgacggagc agaaccgcct
     6361 gctgctctac ctgccgccgg agttcgccca cggcttccag acgctccgcg acgacacgga
     6421 ggtcttctac atggtctcgt cgctctacac gcccgagagc tacaggggcg tgcgctggga
     6481 cgaccccgcc ttcggcatcg agtggcctca cgccgaccgc cgcatcatca acgaaagaga
     6541 ccgcacttac cccgacttca ctccgccgtc gaagctctga acgacaaggc aagcacacgt
     6601 ttcaggactc cccgcccgcc tccgaatccg gaccgccttc gcacgctgcc gcaccccgcg
     6661 acggtttcga ccggcgccgc ctcatcaaca ccactccgcg cacacgccgt tttgcctccg
     6721 cgctcgccgc ccgcccgcgt ctttggagat tttttgga
//
LOCUS       DBNGOH010000178        10034 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3876237, whole genome shotgun sequence.
ACCESSION   DBNGOH010000178 DBNGOH010000000
VERSION     DBNGOH010000178.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 10034)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10034)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10034
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3876237"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            63..1004
                     /gene="fmt"
                     /locus_tag="AC159P_03315"
     CDS             63..1004
                     /gene="fmt"
                     /locus_tag="AC159P_03315"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020712238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HIR3694514.1"
                     /translation="MKLIFMGTPESAVPSLKRCVDDGHEVLAVWTQPDRPAGRGNKLK
                     APPVKEFALAHGLFVHQPAKIKTDEAFALFSSYDVDAVVVVAYGRILPAAYLHAPRHG
                     CVNVHFSLLPKYRGAAPVNWAVVRGERETGVTTMLMDTGLDTGPVLLQRETEIGERET
                     APQLLERLSLVGAELLGETLSRLNELEPRPQDDAEATLAPILKREDGLIDWTRDASDI
                     ERRVRGLQPWPNAFTFHNGRRLVVWRAAPIPPRGDAGTHPDLQAGSVFLAQGELVVGC
                     GGGTALRLDEVQLEGKQRMSARDFANGMHWLGGEQLG"
     gene            1123..2508
                     /gene="rsmB"
                     /locus_tag="AC159P_03320"
     CDS             1123..2508
                     /gene="rsmB"
                     /locus_tag="AC159P_03320"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: protein motif:HMM:NF011494.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HIR3694515.1"
                     /translation="MGTHGGISGKGRDRARGVRVVSPARAAAFEVLRRVEEEGAFAAP
                     LLAGLAEALSGEDRALCYELVLGVLRRQLWLDKALEHYAGRKSESLDAPVRRALRLGL
                     YQLRFLTRIPARAAVNESVNMAHARRMRSAAPFINAVLRRATREPDFDPASRAESLLE
                     RIAVETSHPVWLVERWAASFGTEEAAGFARANNAPAPLAFRVNTLRAGGADTLPKLRA
                     AGIEVAPSRVAPGAYRVRGEGGGAALRALAAEGAVYMQDEASQLVAHVVGARAGERVL
                     DVCAAPGSKTTHVAALACDEAKVTAGDLYEHRLHLVREACARLGVRGVSAVVLDAEDA
                     LPFADGAFDRVLVDAPCTGTGTLRHNPEIRWRLAPTDIAELAARQRRILAEASRTVRR
                     GGRLVYSTCSVEREENESVVADFLASREDFRQVAARPAPDELLAPTGAARMWPHRQDT
                     DGFFVAVMEKQ"
     gene            2721..3428
                     /locus_tag="AC159P_03325"
     CDS             2721..3428
                     /locus_tag="AC159P_03325"
                     /inference="COORDINATES: protein motif:HMM:NF015734.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HIR3694516.1"
                     /translation="MASSTRRILSAAGKLGIVAIVVVAFTFGLVLTVYLSLRSPKIQV
                     PDVVNQQYLEGETTLEKSGLNMRERARRYKPDVPPGVILDQSPRAGEVVKAGQTIAVV
                     ISRARKEGEQPPSEEVAEERKKEETGEARKANENAAKNENRAPRNRNTNRNANAANGN
                     RNADAPNVNAANRNTGNANTRPANSNANRGANTNRNADDGASRPRVTTNRNTSPGGAR
                     NANSARPAGNSNRRPNL"
     gene            3548..4210
                     /gene="rpe"
                     /locus_tag="AC159P_03330"
     CDS             3548..4210
                     /gene="rpe"
                     /locus_tag="AC159P_03330"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011339906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HIR3694517.1"
                     /translation="MVEIAPSILSADFTRLAEEIAAVERGGATIVHVDVMDGHFVPNI
                     TVGLPVVRSLARATQLPLDTHLMISEPGRYAEQFVEAGAQMVSVHVEADPHIHRTLAA
                     IRKKGAKAGIVLNPATSLSALDEVLQYADYVLLMSVNPGFGGQQFIPASVERVRRLRR
                     MIEERGLETRIEIDGGIDLTNIEKVAAAGAEIMVAGTAVFGQGDPAGAVRGLLNATAQ
                     LV"
     gene            4286..5926
                     /locus_tag="AC159P_03335"
     CDS             4286..5926
                     /locus_tag="AC159P_03335"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HIR3694518.1"
                     /translation="MRLRKVALVLLCAVLLAPLLPAASAQTGQQQSGLSPAQRTEVLR
                     SRLETLRRTLNSAIAGLNAKDDGQKDSPRARLSGLEKEAGRLLSEVSDLRGKQERAER
                     FETSDVERLEAAATDLDTRVQSAMRETASARTSTAATASADDDKKKKKKGGFFSRILG
                     RGDDDKYDELVGTVAPGRDRQLFEDATKEARKDNYETARGLYGVIINTYPDSPYLPLA
                     KLAIADTFYLEGTTSALIQAGQHYQDWFTFFPTHPLADDVMIKMAEVEMRQMGLPDRQ
                     VTNAKKAEQRLRVFLQQFPKSSLRPEVEIRLREVQENLAMHDLNTGNFYRARYYKGGA
                     TNLKGAQSRYKEVADKYPHFSRMAEVLYKLGETYVVEEEPDEAAKHYQRLVRNYPNCE
                     VAEKAREQLAAIGASIPEPDPREAAKPCDEGGQGFMGGIWQEITGVVQKTVDKNGVII
                     ANDDKKADLISQAIENGGTLPENFSTQPIERTAPGRRLAPNEQPAPAKPADATKKEFT
                     VEPTQPGAPKSGSDPTRAPATQPTAPATPPPADSGVKP"
     gene            6105..6614
                     /locus_tag="AC159P_03340"
     CDS             6105..6614
                     /locus_tag="AC159P_03340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694519.1"
                     /translation="MAGDLETVQDELTRRHRAAVMFIVYVFALTVLLVLLAFARLFDI
                     DGSGSLAGALRIVIAVFGLGAVMMRRTMFSAMRLKDIAALGGPAALLETLYKTTVYVA
                     LLAAAIAVMAFVISLITGDPADMIWFGLVAVAVLLYAYPRRAAWQSVLRMTAPPASPD
                     ATAAKGTIA"
     gene            6611..8704
                     /locus_tag="AC159P_03345"
     CDS             6611..8704
                     /locus_tag="AC159P_03345"
                     /inference="COORDINATES: protein motif:HMM:NF024821.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3694520.1"
                     /translation="MKPNSHVALSLRAAGCLCSSAILFAAAMTASGANAQGPKIAGES
                     ARAASAQAQQPAAKPSPATAQTQPGQTARERRAQAYAKLLEGQRYYAAARTGQLSLND
                     LQRAQAAFRQAAELDPTLAEAHTALAEIAFFFLDDMPQAEREAEAALRINKDNFGALR
                     VLSRVHTLKSNLAEGRPDAAFAEKAVASLRELLRVRPNDPEAWALLGEFHLLAGREKE
                     AIEAFDKWATLPGSLESRFFQIVTRGRELTPDAANARLAELLLRAGRTAEAVAAIRRA
                     MAIAPENPAYLAMLGEALEASGTVDQTVINELRSIVAQEPKNSAAVGVLARAEARAGR
                     YEEAVAALRAGLAARAGEAREQFNLRLQLAETLADASRFDEAIGIYEELLKERKIGAA
                     LLASERDKQFASAVLRSIAAIQQQAGQDDKALATVARLHTLLGNDAATDALIVSLIRS
                     LGKRQEALASARDARKRYPDDAQLMRLEAITLSESGSVDEALQLMRGRLKGVPADYDE
                     YVIIASILMEAGRGAEAVEAARKALELAPSDELGQNTTALLLLSSAQERAGDAKGSEE
                     TLRGILAKDPKNPSALNNLGYFLTERNERLPEALDMIQKAVRAEPANASYLDSLGWVY
                     FKLGKLTEAERYLRDAARRNPASATIQEHLGDLLQRLGKTEQARAAWQRALSLSVEAA
                     DTSRIKAKLNGGSNK"
     gene            9025..9693
                     /locus_tag="AC159P_03350"
     CDS             9025..9693
                     /locus_tag="AC159P_03350"
                     /inference="COORDINATES: protein motif:HMM:NF012415.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HIR3694521.1"
                     /translation="MRINKQFPLSTPSARAFKTLLLAALVGLTSVAALANGTSKPVAR
                     PVARLLSTSKQEVSSPTRARLLARAAAATSTPVAAAGTSRKSIAIAATSDEQRAFDMI
                     NAERRAQGRGPLVIDGELTRMARYHSENMARRGFFNHVDRDGLDLTGRASVLGLSGWK
                     ALGENIAYNQGYDDPTAFAVERWMISNKHRENILNREFTHAGIGIAKAADGSVFFTQV
                     FMTR"
     gene            9766..>10034
                     /gene="ipk"
                     /locus_tag="AC159P_03355"
     CDS             9766..>10034
                     /gene="ipk"
                     /locus_tag="AC159P_03355"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="An essential enzyme in the nonmevalonate pathway of
                     isopentenyl diphosphate and dimethylallyl diphosphate
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HIR3694522.1"
                     /translation="MSDRTFTLPAFAKINLTLLVHGRRADGYHEIGTTLQTITLRDRL
                     TFEPLEDERVELVCAAPDVPADRTNLVHRAATALRERFGTRRGARV"
BASE COUNT         1795 a         3528 c         3250 g         1461 t
ORIGIN      
        1 agaggggcgg ctccggcgtc gcccttcttc gcgccgtctc tcgcccgcat ccttcatcac
       61 ttatgaaact gattttcatg ggcacgccgg aatccgccgt gccgtctctg aagcggtgcg
      121 tggacgacgg tcacgaggtg ttggccgtgt ggacgcagcc cgaccggccc gcggggcgcg
      181 gcaacaagct gaaggcgccg cccgtgaaag agttcgcgct cgcgcacggc ctcttcgtcc
      241 accagcccgc gaagataaag accgacgaag cgttcgcgct cttctcttcg tacgacgttg
      301 acgcggtggt cgtcgtcgcc tacggtcgca tcctgcccgc cgcatacctc cacgcgccgc
      361 gtcacggctg cgtcaacgta cacttctccc tgctgccgaa gtatcgcggc gccgcgcccg
      421 tcaactgggc cgtcgtgcgc ggcgaacgag agacgggcgt cacgacgatg ctcatggaca
      481 cgggtctcga caccggcccc gtcctcttgc agcgcgagac ggaaatcggc gaacgggaga
      541 ccgcgccgca actgctcgaa cgactctcgc tggtcggcgc ggaactgctg ggcgaaaccc
      601 tgtcgcgcct gaacgagctt gagccgcgcc cgcaggacga cgcggaggcg acgctcgcgc
      661 cgatactgaa gagggaagac gggttgatcg attggacgcg cgacgcctcc gacatcgagc
      721 ggcgcgtgcg cggcctccag ccgtggccga acgcgttcac gttccacaac gggcggcgcc
      781 tcgtcgtctg gcgcgcagcg cctatacctc cgcgcggcga tgcgggcacg caccctgacc
      841 ttcaagccgg atccgtcttt ttagcacagg gcgaactcgt cgtcggttgt ggcggcggaa
      901 ccgcgctgag gctcgacgaa gtccagctcg aaggcaaaca gcgaatgagc gcgagagact
      961 tcgccaacgg gatgcattgg ctcggcggcg agcagttggg gtgaatagat ttgtaggggc
     1021 agggcaagtc cgcggcccgc ggtcacacat tcactcggcc ttgaattcgg cgaagcaaga
     1081 cggacaggga caagccctgc ccctacaaac ttatcgcgcg gcatgggcac acacggaggc
     1141 atcagcggga agggaaggga tcgcgcgcgc ggcgtgcgcg tcgtgtcgcc ggcgcgggcg
     1201 gcggcgttcg aggtgttgcg gcgggtggag gaggagggcg cgttcgcggc gccgctgctg
     1261 gcgggtctgg cggaggctct gagcggcgaa gaccgcgcgc tgtgttacga gctggtgctc
     1321 ggcgtgctgc ggcggcagct ctggctcgac aaagctctgg agcactacgc gggccgcaag
     1381 agcgagagcc tcgacgcgcc cgtgcggcgc gcgttgcggc tcgggctgta ccagctccgc
     1441 ttcctcacgc gcatccccgc gcgtgcggcg gtgaacgagt cggtcaacat ggcgcacgcg
     1501 cggcggatgc gctcggcggc gcccttcatc aacgccgtgc tcaggcgcgc gacgcgcgag
     1561 cctgacttcg acccggccag ccgcgccgag agccttcttg agagaatcgc tgtcgagaca
     1621 tcacatccgg tgtggctcgt cgaacgctgg gccgcgtcgt tcggcacgga agaggccgcc
     1681 ggcttcgcgc gtgcgaacaa cgcgcccgcg ccgctcgcgt tccgcgtcaa cacgctgcgc
     1741 gcgggcgggg cggacacttt accgaaactg cgcgcggcgg gcatcgaggt cgcgccctcg
     1801 cgcgtcgcgc ccggcgcgta ccgcgtccgg ggcgagggcg gcggcgctgc gcttcgcgcg
     1861 ctcgcggctg agggggcggt ctacatgcag gacgaggcgt cgcagctcgt cgcgcacgtc
     1921 gtcggcgcgc gggcgggcga gcgggtgctg gacgtgtgcg ccgcgccggg cagcaagacg
     1981 acgcacgtcg cggcgctcgc gtgcgacgag gcgaaggtga cggcgggcga cctctacgag
     2041 caccgcctgc atctggtgag agaggcgtgc gcgcggctcg gcgtgcgcgg cgtcagcgcg
     2101 gtcgtgttgg acgcggaaga cgcgttgcct ttcgccgacg gcgcgttcga ccgcgtgctc
     2161 gtggacgcgc cctgcacggg caccggcacg ctccgccaca acccggaaat ccgctggcgg
     2221 ctcgcgccca cggacatcgc ggagctggcc gcgaggcagc gcaggattct ggcggaggct
     2281 tcgcgcacgg tgcgtcgcgg cggtcgcctc gtctactcga cctgctcggt cgagagggag
     2341 gagaacgaat cggtcgtcgc cgacttcctc gcctcgcgcg aagacttcag gcaggtcgcc
     2401 gcgcgccccg cccccgacga actgctcgcg cccacgggcg ccgcgcgcat gtggccgcac
     2461 cgacaggaca cggacgggtt cttcgtcgcc gtaatggaaa agcagtaggg gagaaaggga
     2521 gacggggaga cgaggttgat gaggtgacgt ttgacaacac agattctttc tccgcttctc
     2581 cgcttctccg cttctcccct tctgcctgtt gcttgcgggg cttcgggggc gggtgttaga
     2641 ctgcccatcc tgttacgtgt gcgcgccccg tgcggggcgt tttctctgag agataccgag
     2701 ataaggggcg gcgagggagc gtggcatcgt caacgcgtag gattttgtcg gcagcgggca
     2761 agctcgggat cgtcgccatc gtcgtcgtcg ccttcacgtt cggcctcgtc ctgaccgtct
     2821 acctctccct ccgcagtccc aagattcagg tgccggacgt ggtcaaccag cagtatctcg
     2881 aaggcgagac gacgttggag aagtccgggc tgaacatgcg cgagcgggcg cggcgttaca
     2941 agccggacgt gccgccgggc gtgatcctcg accagtcgcc gcgcgcgggc gaggtcgtca
     3001 aggccgggca gaccatcgcc gtcgtcatca gccgcgcgcg caaagagggc gagcagccgc
     3061 cgtccgaaga ggtcgccgag gagcgcaaga aggaagagac cggcgaggcg cgcaaggcca
     3121 acgagaacgc cgcgaagaac gagaaccgcg cgccccgcaa ccgcaacacg aaccgcaacg
     3181 cgaacgccgc gaacggcaac cggaacgccg acgcgccgaa cgtgaacgcc gccaaccgca
     3241 acacgggcaa cgcgaacacg cgccccgcga actccaacgc gaaccgcggc gcgaacacga
     3301 accgcaacgc cgacgacggc gccagccgcc cgagggtcac gaccaaccgc aacaccagcc
     3361 ccggcggcgc gaggaacgcg aattccgcac ggcccgcagg caactcgaac aggcgcccga
     3421 acctctgatt gtctgaattc gcagcgtccc gacagcacgc ccacaggccg aggttcgaac
     3481 aaagtccgaa aggttccgga gcgcccgccg ctcgcccctc gcggcagtca aaaaccggag
     3541 gaaaatcatg gtagagatcg ccccgtccat cctttccgcc gatttcacgc gcctcgccga
     3601 ggagatcgcg gcggtcgagc gcggcggcgc caccatcgtt cacgttgacg tgatggacgg
     3661 ccatttcgtg cccaacatca cggtcggtct gccggtggtg cgctcgctcg cccgcgcaac
     3721 ccaattgccc ctcgacacgc atctaatgat ctccgagccg gggcgttacg ccgagcagtt
     3781 cgtcgaggcg ggcgcgcaga tggtctccgt ccacgtcgag gccgacccgc acatccaccg
     3841 gacgctggcg gccatcagga aaaagggggc gaaggcgggc atcgtcctca accccgcgac
     3901 cagcctctcg gcgctggatg aagtcttaca gtacgccgat tacgtcctgc tgatgtcggt
     3961 caaccccggc ttcggcgggc agcaattcat cccggcctcc gtcgaaagag tccgccgcct
     4021 gcgccgcatg atcgaagagc gcggccttga gacgcgcatc gagatcgacg gcggcatcga
     4081 cttgaccaac atcgaaaagg tggccgccgc cggggccgag atcatggtcg ccggcacagc
     4141 cgtcttcggc cagggcgacc cggcgggcgc cgtgcgcggc ctcttgaacg cgacagcaca
     4201 gctcgtttga aatgacgagt gacgggaggc acgctgctct ccgtcactcg tcgctcgtca
     4261 cttgttttat tcggaggatt tgttgatgcg tttacgcaaa gtcgctcttg ttcttctctg
     4321 cgcggtgctg ctcgcgccgc ttctgcccgc cgcgtccgcg cagaccgggc agcagcagtc
     4381 gggcctctcg cccgcgcagc gcacagaggt tttgcgctcg cgtctggaga cgctgcgccg
     4441 gacgctgaac agcgccatcg ccggcctgaa cgcgaaggac gacgggcaga aggatagccc
     4501 gcgcgcccgc ctgagcggtc tggaaaaaga ggccgggcgg ctgctctccg aggtcagcga
     4561 cctgcgcggc aagcaggagc gcgccgagcg cttcgagacg agcgacgtcg aacggctcga
     4621 agccgccgcc acagacctcg acacgcgcgt ccagtcggcc atgcgcgaga cggcgagcgc
     4681 gcgcaccagc accgccgcca ccgcctcggc ggacgacgac aagaagaaga aaaagaaggg
     4741 cggcttcttc agccgcatcc tcgggcgcgg cgacgacgac aagtacgacg agctggtcgg
     4801 cacggtcgcg ccgggccgcg accgccaact cttcgaggac gccacgaagg aggcgcgtaa
     4861 ggacaactac gagacggcgc gcggcctcta cggcgtcatc atcaacacct acccggactc
     4921 gccgtatctc ccgctcgcca agctcgccat cgccgacacc ttctacctcg aaggcacgac
     4981 gagcgcgctc atccaggccg gccagcacta ccaggactgg ttcaccttct tcccgacgca
     5041 ccccttggcc gacgacgtga tgatcaagat ggccgaggtc gagatgcgcc agatgggcct
     5101 gcccgaccgg caggtgacga acgccaagaa ggccgagcag cgcctcaggg tcttcttgca
     5161 gcagttcccg aaatcgtcgc tccggcccga ggtcgagatt cgtctgcgcg aggtgcagga
     5221 gaacctcgcg atgcacgacc tcaacaccgg caacttctac cgcgcgcgct actacaaggg
     5281 cggcgcgacg aacctgaagg gcgcgcagtc gcgctacaag gaggtggccg acaagtaccc
     5341 gcacttctcg cgcatggccg aagtgctcta caagctcggc gagacgtacg tggtcgaaga
     5401 ggagccggac gaggccgcca agcactacca gcggctcgtg cgcaactacc cgaactgcga
     5461 ggtggccgag aaggcacgcg agcagttggc cgccatcggc gcctcgatcc ccgaacccga
     5521 cccgcgcgag gccgccaagc cctgcgacga gggcggacag ggcttcatgg gcggcatctg
     5581 gcaggagatc accggcgtcg tccagaagac cgtcgataag aacggcgtca tcatcgctaa
     5641 tgacgacaag aaggccgacc tcatctcgca ggccatcgag aacggcggca cgctgcccga
     5701 aaacttcagc acgcagccca tcgagcgcac cgcccccggt cgccgcctcg cccccaacga
     5761 gcagcccgcg cccgccaagc ccgccgacgc gacgaagaag gagttcaccg tcgagcctac
     5821 gcagcccggc gcgcccaaga gcggcagcga ccccacgcgc gcgcccgcca cgcagccgac
     5881 cgccccggcc acaccgccgc ccgccgacag cggcgtcaag ccctagggga agtggaagag
     5941 ggtaaaaggg gaagagggga aaagggagaa gaagcggcgt gctttctccc ttttcccctt
     6001 tctctttgcc cacccgcgaa gccggtgtta atattttttt tcactcctcc cccgttcgag
     6061 tacgtgacct cggtgcgcgc cgcgcaagac gaaaaggatg ccgtgtggcc ggagaccttg
     6121 agaccgtgca ggacgagctg acgcgccgcc accgcgcggc ggtcatgttc atcgtctacg
     6181 tgttcgcgct caccgtgctc ttggtgctgc tcgcgttcgc acgtctgttc gacatcgacg
     6241 gcagcggcag cctggcgggc gcgctccgca tcgtcatcgc cgtcttcgga ctcggcgccg
     6301 tgatgatgcg gcggacgatg ttctcggcca tgcgcctgaa ggacatcgcc gcgctgggcg
     6361 gccccgcagc gctgctcgaa acgctgtaca agacgaccgt ctacgtcgcg ctgctggccg
     6421 ccgccatcgc ggtcatggcc ttcgtcatca gtctcatcac gggcgacccg gcggacatga
     6481 tctggttcgg cctcgtcgcc gtcgccgtcc tgctctacgc ttacccgcgc cgcgccgcct
     6541 ggcagagcgt cctgcggatg acggcgccgc ccgcttcgcc ggacgcgacg gctgcgaaag
     6601 gaacaatcgc ttgaagccca attcacacgt ggccctttcg ctgagagcgg ctggctgcct
     6661 ctgctcttcg gccatcctct tcgccgcggc aatgacggcg agcggcgcga acgcgcaagg
     6721 cccgaagatc gcgggcgaat ccgcgcgtgc cgcttctgcc caggcgcaac agcccgccgc
     6781 gaagccgtcc cccgcgacgg cgcagacgca gcccggacag acggcgcgcg agcgccgcgc
     6841 gcaagcctac gccaagctgc tcgaaggcca gcgctactac gccgcggcgc gcaccggcca
     6901 gctctctctg aacgatttgc agcgcgcgca ggccgccttc cggcaggccg cggaactcga
     6961 ccccacgctt gcagaggcgc acaccgcgct cgccgagatc gccttcttct tcctcgacga
     7021 catgccgcag gccgagcgcg aggcagaggc cgcgctgcgc atcaacaagg acaacttcgg
     7081 cgcgctccgc gtcctctcgc gcgtccacac gctcaagagc aacctcgccg aaggcaggcc
     7141 cgacgcggcc ttcgccgaga aggccgtcgc ctctctgcgc gagctgctcc gcgtccgccc
     7201 gaacgacccc gaggcgtggg cgctgctcgg cgagtttcac ctgctcgccg ggcgcgagaa
     7261 ggaagccatc gaggctttcg acaagtgggc cacgctgccc ggctcgctcg aaagccgctt
     7321 cttccagatc gtcacacgtg ggcgcgagct gacgcccgac gccgccaacg cgcgtctcgc
     7381 cgaactcctc ctgcgcgcgg ggcgcaccgc cgaggccgtc gccgccatcc gccgcgcgat
     7441 ggccatcgcg cccgaaaacc cggcctacct cgcgatgctc ggcgaggcgc tcgaagcctc
     7501 cggcaccgtt gaccagaccg tcatcaacga gctgcgcagc atcgtcgcgc aggagccgaa
     7561 gaacagcgcc gccgtcggcg tgctcgcccg cgccgaggcg cgcgccggtc gctacgaaga
     7621 ggcggtcgcc gcgctgcgcg ccggcctcgc cgcccgcgcc ggagaggcgc gcgagcagtt
     7681 caacctccgc ctccaactcg ccgagacgct ggccgacgcc tcgcgcttcg acgaggccat
     7741 cggcatctac gaggagctgt tgaaggagcg caaaatcggc gccgcgctcc tcgcgtcgga
     7801 gagggataag cagttcgcct ccgccgtgct gcgcagcatc gccgccatcc agcagcaggc
     7861 agggcaggac gacaaggcgc tggcgacggt cgcgcgcctg cacacgctgc tcggcaacga
     7921 cgccgccacg gacgcgttga tcgtcagcct catccgttcg ctcggcaagc gtcaggaggc
     7981 gctggcctcc gcccgcgacg cgcgcaaacg ataccccgac gacgcgcaac tgatgcgcct
     8041 cgaagccatc acgctctccg aatcgggaag cgttgacgaa gcactccagc tgatgcgcgg
     8101 tcgcctgaag ggcgtccccg ccgattacga cgagtacgtc atcatcgcca gcatcctgat
     8161 ggaggcggga cgcggcgccg aagcggtcga ggccgcgcgc aaggcgctcg aactcgcgcc
     8221 gtcggacgaa ctcgggcaga acacgaccgc gctcctcctc ctctcgtccg cgcaggagcg
     8281 cgccggagac gccaaaggct ccgaggagac cttgcgcggc atcctcgcca aagaccccaa
     8341 aaacccgagc gccctcaaca acctcggcta cttcctcacc gaacggaacg aacgcctgcc
     8401 cgaggcgctc gacatgatcc agaaagcggt gcgcgccgaa ccggccaacg cttcttatct
     8461 cgacagcctc ggctgggtct atttcaaact cggtaaactc acggaggccg aacgctatct
     8521 gagggacgca gcacgccgca acccggcttc ggccacgatc caggagcacc tgggcgacct
     8581 cctccaacga ctcggaaaaa cagaacaagc acgcgccgcc tggcagagag ctttatccct
     8641 ttccgtcgaa gccgccgaca cgagccggat caaggcaaaa ttaaacggcg ggagcaacaa
     8701 ataattgttg ccgtctgcgc cgactaaggg taaagtgtgc ccgccttcag gcgagcaggt
     8761 tatgattcgc gctgtcaaga tttagccttc cttagcgcga gacccccttt gtaacctctc
     8821 gcaccaagtc ttaaacaact gatttacatc aatttggggt gattttaagg gcgaaagtct
     8881 tatttcgtct tcgggcacaa aaataagcgt tgacgtgtag tcaaccgggt tttacaatta
     8941 ccaacagtgt aaccgtcttc cccgacggcc caacttctaa aggctcctca aagcccgttt
     9001 tagatttgga gggatgtccc cctgttgaga attaacaaac agttcccctt aagtacgccg
     9061 tccgcaaggg ctttcaagac cctcttgctc gccgccctcg tcggcctgac aagcgttgcg
     9121 gcgctcgcca acggcacctc caagccggtc gcgcgaccgg tcgccagact cctctccacc
     9181 tccaagcagg aagtctcttc gccgacgcgt gcgcgcctgc tggcgcgggc cgccgccgcc
     9241 acgtctacgc cggtcgccgc ggcgggcacc tcgcgcaaat caatcgccat cgccgccacg
     9301 agcgacgagc agcgtgcctt cgatatgatc aacgccgagc ggcgcgctca gggtcgtggc
     9361 ccgctcgtca tcgacggcga gctgacgcgt atggcccgct accactcgga gaacatggcg
     9421 cggcgcggct tcttcaacca cgtcgatagg gatgggcttg acctgaccgg gcgcgcctcg
     9481 gtgctgggcc tctccggctg gaaggcgctc ggcgagaaca tcgcgtataa tcagggctac
     9541 gacgacccga cggccttcgc ggtcgagcgc tggatgatct cgaacaagca ccgcgagaac
     9601 atcctgaacc gcgagttcac gcacgccggc atcggcatcg cgaaggcggc tgacgggagc
     9661 gtctttttca cgcaggtttt catgacgcgc tagtcccgcc cacatttcta ccgcgaccta
     9721 aaggcggctg gcgctttcga tagaggaggc gccagccgct cgcacgtgtc ggacagaaca
     9781 ttcacgctcc ccgccttcgc caagatcaac ctgaccctgc tcgtccacgg tcgccgcgcc
     9841 gacggctacc acgagatcgg caccacctta caaaccatca cgctccgcga ccgtctcacg
     9901 ttcgagccgc ttgaagacga acgcgtcgaa ctcgtctgcg cggcgccgga cgttccagcc
     9961 gaccgaacca acctcgtcca ccgcgcggcg acggcgctcc gcgagcgctt cggcacgcgg
    10021 cgcggcgcgc gcgt
//
LOCUS       DBNGOH010000179         1984 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1676769, whole genome shotgun sequence.
ACCESSION   DBNGOH010000179 DBNGOH010000000
VERSION     DBNGOH010000179.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1984)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1984)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1984
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1676769"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..362)
                     /locus_tag="AC159P_03360"
     CDS             complement(<1..362)
                     /locus_tag="AC159P_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017318835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acireductone synthase"
                     /protein_id="HIR3694523.1"
                     /translation="MTDATTRAILLDIEGTTTPVAFVYEILFPFARRHAAEFIRERYD
                     SDEMRAALDALEAEHREDDAKGNGPPAWLDATDDARIDAATRYVHWLMERDRKSTALK
                     SLQGKIWEGGYRSGRLKG"
     gene            complement(381..947)
                     /locus_tag="AC159P_03365"
     CDS             complement(381..947)
                     /locus_tag="AC159P_03365"
                     /EC_number="1.13.11.53"
                     /EC_number="1.13.11.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0010308 - acireductone dioxygenase
                     (Ni2+-requiring) activity [Evidence IEA];
                     GO_function: GO:0010309 - acireductone dioxygenase
                     [iron(II)-requiring] activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0033353 - L-methionine cycle [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-dihydroxy-3-keto-5-methylthiopentene
                     dioxygenase"
                     /protein_id="HIR3694524.1"
                     /translation="MAIVRIPGEGKTLYDEEEIRLHLASIRIGYERWEPAHAVAEDAT
                     AEELLAAYAPEIERLKERGGYVTADVIDVSPRTPGLDAMLAKFNREHWHDEDEVRFVT
                     RGRGLFHIRPAEGPLTGVEVEPGDLLTVPRGTHHWFDLCADRDIRAIRLFQDPTGWTP
                     HYTGSGAERDYQPACFGPAHIPPRRVAP"
     gene            complement(1096..1749)
                     /gene="mtnB"
                     /locus_tag="AC159P_03370"
     CDS             complement(1096..1749)
                     /gene="mtnB"
                     /locus_tag="AC159P_03370"
                     /EC_number="4.2.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017711805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033353 - L-methionine cycle [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylthioribulose 1-phosphate dehydratase"
                     /protein_id="HIR3694525.1"
                     /translation="MSDHEHDTNGTGATHEALARLLADAGREFHGRGWALGTSGNFSA
                     VTSRDPLRLAITASGLDKSALTPAQILEIDGRGHVMRGEGRPSDETRLHLSVVRARGA
                     GAVLHTHSVWSTLLSETHAPAGRLVIEGFEMLKGLAGVRTHEHRELLPIIDNSQDMDA
                     LSRSLEEVLERHPAAHGVLLRRHGLYTWGRDLAEARRHVEIFEFLLEVVGRERAVSY"
BASE COUNT          296 a          609 c          710 g          369 t
ORIGIN      
        1 tgacccttca gcctgccgct ccggtagccg ccctcccaga ttttcccttg cagcgatttg
       61 agcgcggtgg atttgcgatc ccgctccatg agccagtgga cgtagcgcgt cgccgcgtcg
      121 atgcgcgcgt cgtccgtcgc gtcgagccac gcgggcgggc cgttgccctt cgcgtcgtct
      181 tcgcggtgct cggcttcgag cgcatcgagg gcggcgcgca tttcgtcgga gtcgtaacgt
      241 tcgcggatga actccgcggc gtgtcggcgc gcgaagggga agaggatttc gtagacgaag
      301 gcgacgggcg tggtcgtccc ttcgatgtcg agcaggatgg cgcgtgtcgt cgcgtccgtc
      361 ataagggctg aagtcgaagc ttacggcgcg acgcggcgcg gcgggatgtg ggccgggccg
      421 aagcaggcgg gctggtagtc gcgctcggcg ccgctcccgg tgtagtgggg cgtccagccc
      481 gtggggtcct ggaagaggcg gatggcgcgg atgtcgcggt cggcgcagag gtcgaaccag
      541 tggtgcgtgc cgcgcgggac ggtcaagagg tcgcccggct cgacttcgac gccggtgagc
      601 ggcccctcag ccgggcggat gtggaacagg ccgcgcccgc gcgtgacgaa gcgcacctcg
      661 tcctcgtcgt gccagtgttc gcggttgaac ttcgccagca tcgcgtcgag gccgggcgtc
      721 cgcgggctga catcgatcac gtcggcggtg acgtagccgc cgcgctcttt gagccgctcg
      781 atctcgggcg cgtaggcggc gagcagctcc tccgcggtcg cgtcctccgc gacggcgtgc
      841 gcaggctccc aacgctcgta gccgatccgg atgctcgcca gatggaggcg aatctcttcc
      901 tcgtcgtaca gcgtcttgcc ctcgcccggt atcctgacga ttgccatcat tcacctccgg
      961 taggcggata gccaatagcg attagccatt agctgttagc tagtggatta gtaattgacg
     1021 attgatgaca gcggcgttcg tctagatcat ctccgttaag ctgggagcta acagctaaca
     1081 gccaatagcc aatagctaat agctaacagc tctttcccgt ccgacgactt cgagcaggaa
     1141 ctcgaaaatc tcgacgtggc ggcgtgcctc ggcgaggtcg cggccccaag tgtagaggcc
     1201 gtggcggcgt aggaggacgc cgtgcgctgc ggggtggcgc tcaagcacct cttcgagcga
     1261 gcgcgagagc gcgtccatgt cctgcgagtt gtcgatgatg gggagcagtt cgcggtgctc
     1321 gtgcgtgcgg acgcccgcga ggcccttgag catctcgaag ccttcgatga cgagacggcc
     1381 tgccggcgcg tgcgtctcgg agagcagcgt gctccacacc gaatgcgtgt gcaggacggc
     1441 gcccgcgccg cgcgcgcgca cgaccgagag gtggagtcgc gtctcgtccg aggggcgccc
     1501 ctcgccgcgc atgacgtggc cgcgcccgtc gatctccaga atctgcgcgg gggttagcgc
     1561 gctcttgtcc agcccgctcg cggtgatggc gaggcgcaga gggtcgcggc tcgtgacggc
     1621 gctgaagttg ccgctcgtgc cgagcgccca gccgcgcccg tggaactcgc gaccggcgtc
     1681 ggccagcagc cgcgcgagcg cctcgtgcgt ggcgccagtc ccgttcgtgt cgtgttcgtg
     1741 gtcgctcatt cgacgcgccg tcttcggcaa gtgtggtcgg acattttatg tctgaacggc
     1801 cttgaatgcc agaacgaagg ggaaggggag aagaggggaa aaggggaaaa ggggaaaagc
     1861 aagttgcttg ttttcctttt cgccttttcc cctcttcccc ctttctacgt ggtgggggca
     1921 ccgaggttga gccattcgag gatgagctgg cgcttgtcgc gcgacatgtc gcgtgtgacg
     1981 ggca
//
LOCUS       DBNGOH010000180         4622 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3142591, whole genome shotgun sequence.
ACCESSION   DBNGOH010000180 DBNGOH010000000
VERSION     DBNGOH010000180.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4622)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4622)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4622
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3142591"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..869
                     /locus_tag="AC159P_03375"
     CDS             <1..869
                     /locus_tag="AC159P_03375"
                     /inference="COORDINATES: protein motif:HMM:NF023695.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3616 domain-containing protein"
                     /protein_id="HIR3694526.1"
                     /translation="LPSRSAGGPAANVVSFDGGTFEASGVAHVPGTDGVLFVDDGRTD
                     EIFWMRLGEGRGQAGAIKAVKLGASVIDLEGITHDGTHFYVVGSQSKSKGGDLAGLVR
                     FRFDAREQRAEGAESVSGLKRFLAENVAELRGMADRKYKDGGINVEGVAWDVRGGRLL
                     LGLRSPVVDGHALVVPLRLRDARGAFSVDNLEVEGAKAIRLPLGGAGIRSIEYDDRAK
                     AFRVITGAGPNTEKLDFKLWEWDGDATRPALRETGSYDRRLKPEGVTRVASGGRDFTF
                     IVFDTSGYAAAD"
     gene            1072..1788
                     /locus_tag="AC159P_03380"
     CDS             1072..1788
                     /locus_tag="AC159P_03380"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013718.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HIR3694527.1"
                     /translation="MRLSAFKPIVVGWISYLRTDLHPALGLLMCAGLAAAALALYAFA
                     GLAEEVHEGDTHHFDRTVFALSNLIASPALTAFMQGVTRLGSNEFLIGAGACALIAFA
                     LAGWRRAAAAFLVTMAGAALLNVLLKSIFQRERPAPFFDTPLPESYSFPSGHALLSFC
                     FYGVLAAVLAARLNSVRARVLVWAAAALLVALIGLSRVYLGVHFPSDVLAGYAAALVW
                     VMAVALADRMLQTRRRRGEK"
     gene            complement(1833..2549)
                     /locus_tag="AC159P_03385"
     CDS             complement(1833..2549)
                     /locus_tag="AC159P_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016860546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR3694528.1"
                     /translation="MNDEAVNGKVALVTGANRGIGLEVTRQLARLGYAVILGARDAAS
                     GEKAARPLAEEGWRVVPKRLDVTEQASIDDVRRWAEESFGKLDVLVNNAAILYDSWQR
                     AVGADLKVVREAVETNTLGPWRMCEAFIPLLRRSGHGRIVNVSSESGSLAGMGGGTPA
                     YAVSKVALNALTRMLADELRGAGVLVNSVCPGWVATEMGGPDAPRTVGEGAASVVWAA
                     TLPDAGPTGGFFRDGEPLPW"
     gene            2853..>4622
                     /locus_tag="AC159P_03390"
     CDS             2853..>4622
                     /locus_tag="AC159P_03390"
                     /inference="COORDINATES: protein motif:HMM:NF051698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR3694529.1"
                     /translation="MRSLTQVFCAALLAIAAGVSPAFSQASSSTAELRGQVTDSDGAA
                     IPNAAVTLTDLGKGTSRTTNTDAEGNYVFLNLLPSSYELKVAAATGGFAPGSTRVELT
                     VGQQANIPIQLGAEGLQESVDVVAGAEVVDTDRTQQSTVINITQITNLPISRRNYLDY
                     ALLTPGVSDADNIADASDFRVAQTPNSGLSFGGNNGRGNLVAVDGGSTNTVSGGVETT
                     ISQEGVQEFQVLRNSYAAEFGGASGGIVNIISKSGTNRYSGSVFGLFRNERFDARNPF
                     DFNPTTKSPFNRQQFGGSIGGPLRRDNTFFFASLERFSQDETTFVNLLNTTNIFQVTA
                     SQNQLFNALTGTPFAPLATSLRGTLTTTATNFPRTVQLFTNASGQFPFESSQTTFGFR
                     FDHNFSDRDNAYLRFNLNDSSFENQAAGALTAVSRGRTLDSLNGGVLFSENHQFSSTT
                     FNELKLQYRYLDFDVIPNDGVGPEFNIEGFGFFGRDIFLPSGTIQRDYDIIDNLSHVR
                     GGHTFKFGGGFQASDVSTNNETFFGGRFNFGPNIPLANLVPAATLTQLRGFINNPANV
                     PDANQRAALLNDINTPINALQAFN"
BASE COUNT          736 a         1739 c         1401 g          746 t
ORIGIN      
        1 cgctgccgtc gcgctccgcc ggcggcccgg cggccaacgt ggtgtcgttc gacggcggca
       61 cgttcgaggc ttcgggcgtc gcgcacgtgc ccggcacgga cggcgtgctc ttcgtcgatg
      121 acgggcgcac ggacgaaatc ttctggatgc ggctcggcga ggggcgcggg caggcgggcg
      181 cgatcaaggc ggtcaagctc ggcgcgagcg tcatcgacct cgaaggcatc acccacgacg
      241 gcacgcactt ctacgtcgtc ggctcgcagt cgaaatcgaa gggcggcgac ctcgcgggac
      301 tcgtgcgctt ccggttcgac gcgcgggagc agcgggccga gggcgcggag tccgtctccg
      361 ggctgaagag gttcctcgcg gagaacgtgg ccgagctgcg cgggatggcg gacaggaagt
      421 acaaggacgg cggcatcaac gtcgagggcg tggcgtggga cgtgcgcggc ggtcggctcc
      481 tgctcgggct gcgctcgccg gtcgtggacg ggcacgcgct cgtcgtgccg ctccgcctgc
      541 gcgacgcgcg cggcgccttc tccgttgaca acctcgaagt ggagggcgcg aaggccatca
      601 ggctcccgct cggcggggcc gggatcagga gcatcgagta cgacgaccgc gcgaaggcgt
      661 tccgcgtcat caccggcgcg gggccgaaca ccgagaagct ggacttcaag ctctgggagt
      721 gggacggcga cgccacgcgc cccgcgctgc gcgagacagg ctcttacgac cgccggctca
      781 agcccgaagg cgtcacgcgc gtcgcctcgg gcgggcgcga cttcaccttc atcgtcttcg
      841 acacgagcgg ctacgccgcg gcggattaag agatcgagag cgcctgacaa gacctcggac
      901 ggcacgaatg gttgtagggg cagggcttgt ccctgcccgt tcaagtttcg tgaggtctca
      961 aggttgcagc ccgtcctgag cacaggcagg gacaagccct gcccctacag ttttttcatc
     1021 acaaaggtct tctggccgcc ggactgtcga gccgccgggg ctattcaacg agtgcgcctt
     1081 tcagccttca agccgatcgt cgtcggctgg atcagttatc tcaggacgga cttgcacccc
     1141 gcgctggggt tgctgatgtg cgccggactc gcggcggcgg cactcgccct ctacgccttc
     1201 gccgggctgg ccgaggaggt gcacgagggc gacacacacc acttcgaccg gacggtcttc
     1261 gcgctctcga acctgatcgc gtcgcccgct ctcaccgcct tcatgcaggg cgtgacgcgg
     1321 ctcgggtcga atgagttcct gatcggcgcg ggcgcctgtg ccctgatcgc cttcgcgctc
     1381 gccgggtggc ggcgcgcggc tgccgccttc cttgtgacga tggccggggc ggcgctgctc
     1441 aacgtcttac tcaagtcgat cttccagcgc gagaggcccg cccccttctt cgacacaccg
     1501 ctccccgaat cgtacagctt tcccagcggg cacgcgctgc tctcgttctg cttctacggg
     1561 gtgttggccg ccgtactcgc cgcgcgcctg aacagcgtcc gcgcacgggt gctggtgtgg
     1621 gccgccgcgg cgctactcgt cgccctcatt ggcctctccc gcgtctacct gggcgtccac
     1681 ttcccgagcg acgtgctggc agggtacgcg gcggcgctcg tctgggtgat ggccgtggcg
     1741 ctcgccgacc ggatgctaca aacccgccgc cgccgaggtg aaaagtaagc catcgcctca
     1801 agcacaagcg ggccgaccca tccgccgacc gctcaccacg gcagcggctc gccgtcgcgg
     1861 aagaagccgc cggtcggccc cgcgtcgggc agtgtggccg cccacacgac gctggccgcc
     1921 ccctccccca ctgtgcgcgg cgcgtccggc ccgcccatct cggtcgccac ccagccgggg
     1981 cagaccgagt tgacgagcac gcccgcgccg cgcagctcgt ccgccagcat ccgcgtcagc
     2041 gcgttgagcg cgaccttcga gacggcgtag gccggtgtgc cgccgcccat ccccgcgagc
     2101 gaccccgact cgctcgacac gttcacgatc cgcccgtgcc cgctccgccg cagcagcggg
     2161 atgaacgcct cgcacatccg ccacggcccg agcgtgttgg tctcgaccgc ctcgcgcacc
     2221 accttcaagt ccgcgccgac cgcccgctgc cacgaatcgt agaggatcgc cgcgttgttg
     2281 acgagcacgt cgagcttgcc gaaactctct tccgcccagc gcctcacgtc gtcgatgctc
     2341 gcctgctccg tcacatccaa gcgcttcggg acgacccgcc acccctcttc cgccaacggg
     2401 cgcgcggcct tctcgccgct cgccgcgtcg cgcgcgccga gaatcaccgc gtagccgagc
     2461 cgcgcgagct gacgcgtcac ttcgaggccg atgcccctgt tcgcgcctgt cacgagcgcg
     2521 acttttccgt tcaccgcttc gtcattcatc ttcttctcga cctcgcccct tccgcacgac
     2581 cattcgtaga gcgacacgcg cccggcggat tcgttccggc gcgcgcccga gccgcgccga
     2641 cgccgccggc ccgaggataa caaaagcttg acgtttacag gtgccggtgt tactttaccg
     2701 accgttcggt agccattgcc gcgtctcgga ctccggtcca ctccctttag gtttcttcga
     2761 ggttaatcgc cgcgtccctc gcacgcgccg cgtccggtgt cctggctgcc cgaccgtcac
     2821 cacttctctt caacctaagg agattaactg caatgagaag cctgactcag gttttttgcg
     2881 cggcgctgct cgccatcgcc gccggagtct cccccgcttt ctcgcaggca tccagctcga
     2941 ccgcggagct gcgcggccaa gtcaccgact ccgacggcgc ggccatcccc aacgccgccg
     3001 tcacgctcac ggacctcggc aaagggacga gccgcacgac caacaccgac gccgagggca
     3061 actacgtctt cctcaacctg ctgcccagct cctacgagtt gaaggtcgcg gcggcgacgg
     3121 gcggcttcgc gcccggctcg acgcgcgtcg agttgacggt cggccagcag gccaacatcc
     3181 ccatccagct cggcgcggaa gggctacagg agagcgtgga cgtggtggcc ggggccgaag
     3241 tggtggacac cgaccgcacg cagcaatcga cggtcatcaa catcacccag atcaccaacc
     3301 tgccgatcag ccgccgcaac tacctcgact acgcgctctt gacgccgggc gtttcggacg
     3361 ccgacaacat cgccgacgcc tctgacttcc gcgtcgcgca gacgcccaac tccggcctct
     3421 ccttcggcgg caacaacggt cgcggcaacc tcgtcgcggt ggacggcggc agcaccaaca
     3481 cggtctcggg cggcgtcgag acgaccatca gccaggaggg cgtgcaggag tttcaggtct
     3541 tgcgcaacag ctacgccgcc gagttcggcg gcgcctcggg cggcatcgtc aacatcatct
     3601 ccaagtccgg cacgaaccgc tacagcggca gcgtcttcgg cctcttccgc aacgagcgct
     3661 tcgacgcgcg caaccccttc gacttcaacc cgacgaccaa gtcgcccttc aaccgccagc
     3721 agttcggcgg ctccatcggc ggccccctca ggcgcgacaa taccttcttc ttcgcctcgc
     3781 tcgaacggtt cagccaggac gagacgacct tcgtcaatct cctcaacacg accaacatct
     3841 tccaggtcac ggcgtcgcaa aaccagctct tcaacgcgct gaccgggacg cccttcgccc
     3901 cgctcgccac cagcttacgc ggcacgctga cgaccaccgc gaccaacttc ccgcgcaccg
     3961 tccagctctt caccaacgcc agcgggcagt tccccttcga gtcgagccag accaccttcg
     4021 gcttccgctt cgaccacaac ttcagcgacc gcgacaacgc gtacctgcgc ttcaacctca
     4081 acgactccag cttcgagaat caggcggcgg gcgcgcttac cgccgtctcg cgcgggcgga
     4141 cgctcgactc gctcaacggc ggcgtgctct tctccgagaa ccaccagttc agcagcacca
     4201 ccttcaacga gctgaaactc cagtatcgct acctcgactt cgacgtgatc cccaacgacg
     4261 gggtcggccc cgagttcaac atcgagggct tcggcttctt cgggcgcgac atcttcctcc
     4321 cctcgggcac catccagcgc gactacgaca tcatcgacaa cctctcgcac gtgcgcggcg
     4381 gccacacctt caagttcggc ggcgggttcc aagcctcgga cgtttccacg aacaacgaga
     4441 ccttcttcgg cgggcgcttc aacttcggcc ccaacatccc gctcgccaac ctcgtcccgg
     4501 ccgcgacgct gacgcaactg cgcggcttca tcaacaatcc cgccaacgtc ccggacgcca
     4561 accagcgcgc cgcgctgctc aacgacatca acacgccgat caacgcgctt caggctttca
     4621 ac
//
LOCUS       DBNGOH010000181         8138 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_759619, whole genome shotgun sequence.
ACCESSION   DBNGOH010000181 DBNGOH010000000
VERSION     DBNGOH010000181.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8138)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8138)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8138
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_759619"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(224..1387)
                     /locus_tag="AC159P_03395"
     CDS             complement(224..1387)
                     /locus_tag="AC159P_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005252438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HIR3694530.1"
                     /translation="MDAKGGAREKYDEIRDGLIALSHRIHAHPELGFEEVKASAWLCE
                     FLADAGFKVERGVCGLPTAFVARAGDGPLHVAICAEYDALPGIGHACGHNIIAAMSTG
                     AGIAAARVAADLGLTVSVIGTPAEEVVNHGGKIHLLEGGAFAGVHAAMMVHPAPFDVV
                     TPRIIAASMFDAHYTGKESHASAFPELGVNAADALTVAQTAIGLLRQHILPTQRIHGI
                     ITKGGDAPNVIPAHTFAKYIIRGQTLEELEELRPKVHRCFEAGAVATGATLSVAGGDK
                     PYAEMNHDGELAAAYRRNAEALGRTFPDLGVVLERAAGSTDMGNVSLALPAIHPFVGI
                     NSLPAVNHQPEFTAHCIKEEADQALHDGALALAWTAIDTAQDDGLRRRLTARC"
     gene            complement(1695..2462)
                     /locus_tag="AC159P_03400"
     CDS             complement(1695..2462)
                     /locus_tag="AC159P_03400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694531.1"
                     /translation="MQPTAVPIVDERTIRHEGKSPSAMSPLGEAEKEAIVRAEYFPKA
                     HRIGLITSLLHVVIFFLPPIYLMLFYGLPADWGRIMQGAAATWSTSMPFWFIEPVSYF
                     LVLGTCGTYISFLAGNIANFRLPVSAVAQEVAQVKEGSHEGEIISALAIAVTQMMITV
                     SALTGAIFVSAIVGLLPVPVVAAFDWLLPSIWGAIVAQFGLRNWRYAAVAVTASIVVV
                     TYSGLPGWSHIPILVVMMVALALLTHKRRIWMSKETD"
     gene            complement(2496..3233)
                     /locus_tag="AC159P_03405"
     CDS             complement(2496..3233)
                     /locus_tag="AC159P_03405"
                     /inference="COORDINATES: protein motif:HMM:NF027796.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5058 family protein"
                     /protein_id="HIR3694532.1"
                     /translation="MEPEWIKVATSPGFWVIGFIFAGNALIQSYLFFRLSRRMGTRLR
                     MGEGGTREAIKASFIASLGPAFGSFVGMTVLVLALGGAYAFARESAGVGSIMYELIAA
                     RAGAEAAGVPLTREGMTLPALLIIFWVTALGSFGWGLIGGVFTRWLPKIKDLMGGGDT
                     TRLRRISVAIMLAAFGRLLANDSVAPLLRDERWPAMIASLVGGGASLAWLLYADRANK
                     PALKEYSLIIALVLGMTAGQITHMVFE"
     gene            complement(4155..4592)
                     /locus_tag="AC159P_03410"
     CDS             complement(4155..4592)
                     /locus_tag="AC159P_03410"
                     /inference="COORDINATES: protein motif:HMM:NF024865.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR3694533.1"
                     /translation="MVVAVHSFVFNQPALGGANQKGADEQAVRQVLDELYAALGRNDD
                     AALDRIYADDYTLVNESGGLTTKAPRLAAIRSGELKFESAGYDEANIRLYGNTAVATY
                     RVMIKGQFKGQDVGGQLRVTSTLVKTKARWQLVATQATSIGGR"
     gene            complement(5042..5614)
                     /locus_tag="AC159P_03415"
     CDS             complement(5042..5614)
                     /locus_tag="AC159P_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017666877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR3694534.1"
                     /translation="MAKPSKLAHVVYMTRRFDEMLDWYQKVFETEVQYRNPAFAFLTF
                     DDEHHRFAFANMSVISPEGAANELAHGVGVNHVGYTYEKAGDLLDTYARLKGLGITPY
                     WEVHHGVTLSVYYRDPDGNRMEFQVDCCADAEEAHAFMQSDAFAANPVGVEVDFDELL
                     TRYRGGVPEETLLAPPAGPVSSIPPAHGLF"
     gene            complement(5727..6056)
                     /locus_tag="AC159P_03420"
     CDS             complement(5727..6056)
                     /locus_tag="AC159P_03420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694535.1"
                     /translation="MKPGKETAYLNYIATDWKREQAALKKDGVIVSYKVLTTEPHDPQ
                     DFNILLMTEYKGLATMEANEQKEEAVAQALIGNDEKQRQGYRERLEIREVLGIRLARE
                     MVPEPRP"
     gene            complement(6798..>8138)
                     /locus_tag="AC159P_03425"
     CDS             complement(6798..>8138)
                     /locus_tag="AC159P_03425"
                     /inference="COORDINATES: protein motif:HMM:NF012956.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M36 family metallopeptidase"
                     /protein_id="HIR3694536.1"
                     /translation="FSNMGYDPFFDLAGGLTPVHADGEIWSATNFDIRQALVEKYNAT
                     HPASDAAAQRACAEGRKAPQDCPGNRRWVQLMYDAYLLMPTAPTMLQARDAYLAADMM
                     RFGGANQAELWLAIARRGFGQGAAATTTHAGSDTDPKPDFASPRHSNATVTFKALASD
                     EANGEIANARVFVGRYEARVSPVADTNPNTSAEGGGANNLDAVAQFAPGTYEFVVQAP
                     GYGHVRLTRTFTAGTTQTLTVLMATNLASASKGATAAGGGNVADLIDDTENTTWTAAG
                     APSAQQVTIDLAGTAPQAVNRVQVSSMLQTGQNRFTALRQFRVEVSVDGTTFTPVFTS
                     PSDAFPGASPRPGVPDMIIRSFSFPAVPATQVRLVVLHTQCTGQSEFHGIQDDDLATG
                     TDCRGEGEPAGTDPLGASGQTPKPQNTNTRAAEIQVFKSEGGVDGGGKGKPKGR"
BASE COUNT         1393 a         2638 c         2670 g         1437 t
ORIGIN      
        1 ctggcgcgca cagcggcggc cggggcacgg caggccgggg cgcagggggt tgaggcttac
       61 cgaggcgggc ggcgttcgga ccgcagtcgc gttcgacatt ttcgtaccac gcctgccctc
      121 tgcaacaaac cggtctcctc ttgccttccc tcacgtccgg gtgcatgccg ttgtttgggc
      181 taccccggag agggccctct gtacacctgc tgacactaac tccctaacat ctggctgtca
      241 ggcgacggcg cagtccgtcg tcttgggccg tgtctatcgc ggtccaagcc agcgcgagcg
      301 cgccgtcatg aagggcctga tcggcctctt ccttgatgca gtgggcggtg aactccggct
      361 ggtggttgac ggcaggcagc gagttgatcc cgacgaacgg gtgaatggca ggcagggcca
      421 gcgacacgtt tcccatatcg gtcgacccgg ccgcccgctc gagcaccacg cccaagtcgg
      481 gaaaggtgcg acccagcgcc tccgcgttac gtcggtatgc ggcggccagc tccccgtcgt
      541 ggttcatttc ggcgtaaggc ttgtccccgc cggccacgct caaggtcgcg ccggtggcga
      601 ccgcgccggc ctcgaaacac cggtgcacct tcgggcggag ttcctcaagt tcttctaacg
      661 tctgaccgcg aatgatgtat ttagcgaacg tatgggccgg gatcacattc ggcgcgtccc
      721 cgcccttcgt gatgatcccg tggatgcgtt gggtcggtag tatgtgctgg cgcagcaggc
      781 cgatcgcggt ttgcgcgacg gttaaggcgt cagccgcgtt aacccccaac tcagggaatg
      841 ccgaggcgtg cgattccttg cccgtgtagt gggcgtcgaa catcgaggcg gcaatgatgc
      901 gcggcgtgac gacgtcgaag ggtgcgggat ggaccatcat cgccgcatgt actcccgcga
      961 acgccccccc ttcgagtaga tggattttgc ccccgtggtt cactacctct tcggccggtg
     1021 tgccgatcac gctgacggtc aggccgagat cggcagccac ccgggccgcc gcgatcccgg
     1081 ccccggtact catcgccgcg atgatgttgt ggccgcacgc gtgaccgatg ccgggcaggg
     1141 cgtcatactc ggcgcagatg gcaacgtgta gagggccgtc accggcgcgc gcgacgaatg
     1201 ccgtcgggag gccgcagacg cctctttcaa ccttgaatcc tgcgtctgcc aaaaattcgc
     1261 agagccacgc ggatgccttt acttcttcga acccgagctc ggggtgggca tggatccgat
     1321 gactgagggc gatcaggcca tcgcgtatct catcgtattt ctcccgagcg ccgcctttcg
     1381 catccatgat cattctcctt tccaggcttt aaaactccga tccgcatcgt cctgaaatga
     1441 ctggtctcga tcgtggtttg gcaccctcac ggtttaaagt ttcggctctg cgcctaacgc
     1501 cgggcctcac ccgccgcgcg ttcaaaacag gtatggccaa gtctcgcgga tgaaggccgc
     1561 tattcgcgga cgggtgcagg ccgctgttag gcgcgggcag tgaggaacgg cgcccttccg
     1621 tgcgctgaca tccgcgacgt tataggaact tggacatgcc cgtcggcccg gcgcctgccc
     1681 caggggcggc acccctaatc cgtctctttg gacatccaga tgcggcgttt gtgggtgagc
     1741 agcgccagag cgaccatcat cactaccagg attgggatgt ggctccagcc gggcaggccg
     1801 gagtaggtga ccacgacgat ggaggcggtg accgccaccg ccgcatagcg ccagttgcgc
     1861 aagccgaact gcgcgacgat ggcgccccag atcgaaggca gtagccaatc gaacgccgcc
     1921 acgaccggca ccggcaggag gccgacaatg gcgctgacga agatcgcgcc cgtcaaggcc
     1981 gagacggtga tcatcatctg ggtcaccgcg atggcgagcg cggagatgat ttccccctcg
     2041 tgagagcctt ccttaacctg cgccacctcc tgggccacgg cggagaccgg gagtcggaag
     2101 ttggcgatgt tgccggccag gaagctgatg taggtgccgc aggtgcccag gaccaggaag
     2161 taggagacgg gctcgatgaa ccaaaagggc atgctggtgc tccaggtggc ggcggcgccc
     2221 tgcatgatcc tgccccagtc ggcgggcagc ccgtagaaga gcatcaagta aatggggggc
     2281 aggaagaaga tgaccacgtg taacagggag gtgatgaggc cgatccggtg cgctttggga
     2341 aagtactccg cccgcacgat ggcttccttc tccgcctcgc ccagcggact catggccgac
     2401 ggtgacttcc cctcgtgcct aattgtccgc tcatccacga tcgggactgc tgtcggctgc
     2461 atcgctcggg cctcctttaa cgcgttcact tacccctatt caaagaccat gtgggtgatc
     2521 tggccggctg tcatgcccag caccagagca atgatgaggg agtattcctt cagggcgggc
     2581 ttgttggcac gatccgcgta tagcaaccag gctaaggagg cgccgccgcc gacgaggctg
     2641 gcgatcatgg caggccagcg ctcgtcgcgc aggagcgggg cgacgctatc gttggcgagc
     2701 aggcgcccga aagcggccag catgatggcg acggagatcc tccgcagtcg cgtcgtgtcg
     2761 cccccgccca tcaggtcctt gatcttcggc agccagcgcg tgaacacgcc gccgatcagc
     2821 ccccagccga aagagcccag ggcggtgacc caaaagatga tcaggagggc gggcagagtc
     2881 atgccctcgc gggtgagcgg cacgccggcg gcttcggcac ccgcccgggc cgcgatgagc
     2941 tcgtacatga tcgagcctac ccccgcggac tcccgggcga aggcgtacgc gccgccgagc
     3001 gccagcacca acaccgtcat cccgacgaag ctgccgaagg ccggcccgag cgaagcgatg
     3061 aaggaggcct tgatggcttc gcgcgtcccc ccttccccca tccgcagtcg agtccccata
     3121 cgccgcgaga ggcggaagaa caggtagctc tggatcaagg cgttgcccgc gaagatgaaa
     3181 ccgatgaccc agaagcccgg actggtcgcc acttttatcc attcgggttc catatgtagt
     3241 ggcccctcct ctccgaacac ttcatctcag aatagaagtc aggcgcctca gcgcgcattg
     3301 cggaaagaat gccgaaaacg ccatcagcaa aacgtcttga tcccatcacc ggacccgatc
     3361 gtgagcagcg ccgggatgca acccgtcaat gatgcacaac aaagcaaagg ctctctcgta
     3421 cacagccgcg ctcgccgtcc aacgcctgcc ttcacccgcc gcgcaatcag cgtgatgacg
     3481 gccaagtctc gcggtaagaa gaatgctgac tcacggacgg gtgcaggccg ttgtcaggcg
     3541 cgggctgggc cgcacggctc gtgctacggc ctgcaagggt gcttcaactc cacggccaaa
     3601 gcgtgagcct cagttttccc gatgttctca agcgcgtgta ttaaggggtt gcgccaagaa
     3661 acgccccccg ctttccccgt aacctcaacc gcttgaccgt ccgggagagt gctcctcaat
     3721 gtgctgtcgc cgaggaagta cacgaccccg gcagggtgcg agtgcgtcgg ctctttctcg
     3781 cccggtctga ggcgatattc gagcacccga acttgttcgt tctccagcag gactttgtaa
     3841 taccggggcg ataatctgac cggatcctgg gaagacctca cccccgacga gagggcaaac
     3901 acgaaggtgg ccagcaacgc acccagaagc acaccagcga tcaagtgggc agctcgtttc
     3961 atcatgcttt gtccttacct atggcagcac aacccgaagc tcctaacgcc ggaatcgagc
     4021 cgccgcccac gaatggcatt taacgctatc gccaggaaac acgatgagcg ggatgctacg
     4081 gggcggtcgg ctcgaatgaa ctgctcggtt gcgccggcga acgacgcgct tccgtcatgc
     4141 ccctcccacg gccgtcaccg tccgccgatg cttgtggctt gcgtggccac cagttgccag
     4201 cgcgctttcg tcttcacgag tgttgaggtg actcggagtt gaccgccgac atcttgccct
     4261 ttgaactgac ctttgatcat cacgcggtac gttgccacgg ccgtattccc atagagacgg
     4321 atgttcgcct cgtcgtaacc ggcggattcg aacttcagct ctccagacct gatcgccgcg
     4381 agacgtggcg ctttcgtagt caggccgccg gactcgttaa ccagggtgta gtcgtcggcg
     4441 tagatgcggt cgagcgcggc gtcgtcgttg cgccccaagg cagcgtagag ctcatcaagc
     4501 acctgccgga cggcctgctc gtcggcgccc ttttggttag cgccaccgag cgccggctga
     4561 ttgaaaacga aagaatggac ggcgactacc atcgctacta caaggattcg tctcacgaga
     4621 gttctccttc caagggtgcg ccggcgagaa cggcagggat cgggaaggac gcgcggttta
     4681 aatagcaagt cccggggcgc gtccatggta agagctgaaa gcccacgcct cacgaagcaa
     4741 ccgaacgccc ccggatgagc ggcgcgcaca gaccgtcgct aataaaaagc gtgtgccgga
     4801 tatgtgcaac tcagccggac caggtcgcac tcagccacac ggggcgtaag aacggtctca
     4861 ggaaagccta accagggcac acgcgaacca acttggacgc caacaccgcc tggatgtcac
     4921 ccctcaacgc aagcctcata cacgccgcgg actgcttcta cattgctcac tgttctcctc
     4981 cggcaaagtg tttactgcgg cgggcccgtt tcggcccacc cttctccgtc agttccggcc
     5041 ctcagaagag gccatgcgcg ggcgggattg acgagaccgg cccggcgggg ggcgcgagca
     5101 gagtctcctc cgggacgccg cctcggtatc tcgttaacag ttcgtcgaag tccacctcca
     5161 cgccgacggg gttggcggcg aaggcgtcgc tctgcatgaa ggcgtgggcc tcttccgcgt
     5221 cggcgcaaca gtcgacctgg aactccatcc ggttcccgtc cgggtcccgg tagtagacgg
     5281 agagcgtcac cccgtggtgg acctcccagt agggcgtgat tcccaacccc ttgaggcggg
     5341 cgtaagtgtc gagcaggtcg cccgccttct cgtaggtgta gccgacgtgg ttgacgccga
     5401 ccccgtgggc caactcgttc gcggcgcctt cggggctgat gaccgacatg ttggcgaagg
     5461 cgaagcggtg gtgctcgtcg tcgaaggtga ggaaggcgaa cgccgggttc cggtactgga
     5521 cttcggtctc gaagaccttc tggtaccagt cgagcatctc gtcgaagcgc ctcgtcatgt
     5581 agacgacgtg cgcgagtttg gaaggcttgg ccatatcgtg gggctccttc cccgccgagg
     5641 ggagtcgaag gcctgacggg cgggggcttg tgccgtcggg gccgggtgaa cgtgcggttg
     5701 agtccgcttg ttgggaggag ctggcgttac gggcgcggct ccgggaccat ctcccgtgcc
     5761 agcctgatgc ccaggacctc gcggatctcg agccgctcgc ggtagccctg ccgctgcttc
     5821 tcgtcgttgc cgatgagcgc ctgggcgacc gcctcctcct tctgctcgtt ggcctccatc
     5881 gtcgccaggc ccttgtactc ggtcatcagt aggatgttga agtcctgcgg gtcgtgcggc
     5941 tcggtcgtca gcaccttgta ggagacgatg accccgtcct tcttcaaagc cgcctgttcc
     6001 cgcttccagt cggtggcgat gtagttgagg taggccgtct ccttgcccgg cttcatcctg
     6061 atgaacgaga cgttccagac cgagccgttg cggaaggggc ggcggacttg cgcttagacg
     6121 aaggcggcga ggagcaagag ggacgccgct agcgcggcgg cgagtagacg ttcggttctc
     6181 atggcggact cctacccata cgagtggcgt gcggcccacg ggcgatgtac ggggtgactc
     6241 gccccgcggg gccaacattc gaggagctac ccgcttgcgg aatggcaggg cgcgagggcg
     6301 gcgggggttc cgtagacgct agcccagggg cgtctccgtt taagagacga tcagccactt
     6361 gccgccgcgg ttgacccaca cttggcactc tacgcgaggg ggcggcggtc gcacatcgga
     6421 caaagggaca gttccgcgta gtaggaatgt cacggggcgg gcgctggaag ccggggcgtg
     6481 gcttgtagaa gccggcggag ggccttgcct aaaagctgaa ggaagggttg ctgacggaag
     6541 cccggagcgg acccggagcg caaggtgctt gggtaagcac ctcgcgcgga ggggtgcaag
     6601 gctcaaagcc ttgcaagcgg agccggtacg gtgggtatcc gccacgccct cgccgtccgg
     6661 tgctcgtcac actcagccgc ggcggtgttc cggaaagtga cgggggcggg agagacttta
     6721 ctctcccgcc ccccggatca accctcgtga cgtatcaacc accggattcg ggccgacggc
     6781 ggctgcctcg gtgataacta tctgcctttc ggtttgccct tccccccgcc gtccacgccc
     6841 ccctcgctct tgaagacctg gatctcggcg gcgcgcgtgt tcgtgttctg cggcttcggc
     6901 gtctggcccg acgccccgag cggatcggtc cccgccggct cgccctcgcc gcggcagtcg
     6961 gttccggtcg ccaagtcgtc gtcctgaatg ccgtggaact ccgactgccc ggtgcactgc
     7021 gtgtgcagca cgaccagccg cacctgcgtg gccggcaccg ccgggaacga gaacgaccgg
     7081 atgatcatgt cgggcacgcc gggccgcggc gaggcgcccg ggaaggcgtc ggacggactc
     7141 gtgaagaccg gcgtgaacgt cgtcccgtcc accgagacct ccacgcggaa ctggcgcaac
     7201 gccgtgaacc ggttctggcc ggtctggagc atcgagctga cttggacgcg gttgaccgcc
     7261 tgcggggccg tgcccgccag gtcgattgtg acctgctgcg ccgacggggc gccggccgcc
     7321 gtccaggtcg tgttctcagt gtcgtcgatc aggtccgcga cgttcccgcc gccagccgcg
     7381 gtcgcgccct tcgaggccga ggcgaggtta gtcgccatca agaccgtcag cgtctgggtg
     7441 gtgccggccg tgaacgtccg cgtgagccgg acatgcccgt agccgggggc ctggacgacg
     7501 aactcgtagg tgcccggcgc gaactgggcg acggcgtcga ggttgttcgc cccgccgccc
     7561 tcggcgctcg tgttcgggtt cgtgtccgcg accggcgaga cgcgcgcctc gtaacgaccg
     7621 acgaagacac gcgcgttcgc gatctcgccg ttggcttcgt cggaggccag cgccttgaac
     7681 gtcacggtcg cgttcgagtg ccggggcgag gcgaagtcgg gtttcgggtc ggtgtcgctg
     7741 cccgcgtgcg tggtggtcgc cgccgcgccc tggccgaagc cgcggcgcgc gatcgccagc
     7801 cacagctcgg cctgattcgc gccgccgaac cgcatcatgt cggcggcgag gtacgcgtcg
     7861 cgggcctgga gcatcgtcgg cgcggtcggc atgagcaggt aggcgtcgta catgagctgg
     7921 acccaccgcc ggttgccggg gcagtcctgg ggcgccttcc ggccctcggc gcaagcgcgc
     7981 tgcgccgcgg cgtcggacgc cgggtgcgtc gcgttgtact tctccaccag cgcctgcctg
     8041 atgtcgaagt tcgtcgcgct ccagatctcg ccgtccgcgt ggaccggcgt caacccgccc
     8101 gccaggtcga agaacgggtc gtatcccatg ttgctgaa
//
LOCUS       DBNGOH010000182         1727 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2959790, whole genome shotgun sequence.
ACCESSION   DBNGOH010000182 DBNGOH010000000
VERSION     DBNGOH010000182.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1727)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1727)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1727
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2959790"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..697)
                     /locus_tag="AC159P_03430"
     CDS             complement(<1..697)
                     /locus_tag="AC159P_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012632207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIR3694537.1"
                     /translation="MEPLPIDALLPDILARLREAPNLVVEAPPGAGKTTRVPPAMLDA
                     GLAGVGEVWVLEPRRLAARMSARRVAEERGEAVGETVGYQVRFEEVASRRTRLRFLTE
                     GVLTRRLLSDRELSNVGAVVLDEFHERHLQADLALALLRRLQQGERPDLRLVVMSATL
                     APEPLARYLGGCAVLRSEGRRYEVEIEHLARADDRPLAAQVERAARRLFASGLAGDAL
                     VFLPGAAEIRRAQR"
     gene            complement(813..1289)
                     /locus_tag="AC159P_03435"
     CDS             complement(813..1289)
                     /locus_tag="AC159P_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HIR3694538.1"
                     /translation="MDKDKALREHLLYLLRGGGAHLDFESAVAGFTVASINRKVEHLP
                     YTAWQIVEHMRIAQWDILEFSRSAAHTSPPWPEGYWPAPGEEADEAAWQRSLDVFRAD
                     LKAMKALVADPAQDLYAPIPHGDGQTLLREALLVADHNAYHLGALVVLKRLLAVGS"
     gene            complement(1276..>1727)
                     /locus_tag="AC159P_03440"
     CDS             complement(1276..>1727)
                     /locus_tag="AC159P_03440"
                     /inference="COORDINATES: protein motif:HMM:NF017551.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007030 - Golgi organization [Evidence
                     IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NRDE family protein"
                     /protein_id="HIR3694539.1"
                     /translation="AGDADELYYYSNRGGAPHRLSPGVYGLSNHLLDTPWPKVERGKK
                     ALAEVVGGSEALAPESFIDILFDRVRAADAELPDTGVGVELERLLSPLFIVSPSYGTR
                     CSTVVLIDRRGTLTFVERTYGSGAVASGEVGYEFHVGDAEEESLGQR"
BASE COUNT          280 a          594 c          593 g          260 t
ORIGIN      
        1 ccctttgagc gcgccgaatc tccgccgcgc cgggcagaaa gacgagcgcg tcgcccgcga
       61 gacccgaggc gaacaggcga cgcgccgcgc gctcgacctg cgcggcgagc ggcctgtcat
      121 ctgcacgcgc gaggtgttcg atctcgacct cgtagcgcct gccttcggag cgcagcacgg
      181 cgcagccgcc gaggtatcgc gcgagcggct cgggcgcgag cgtcgcggac atcacgacga
      241 ggcgcaggtc tggccgctcg ccttgctgca agcgacgcag cagcgcgagc gcgaggtctg
      301 cttgcagatg cctctcgtgg aactcgtcga gcacgacggc gccgacgttc gaaagttcac
      361 ggtcggagag cagacgcctc gtcagcacgc cttccgtcaa aaagcgcagg cgcgtgcgcc
      421 gcgacgcgac ctcctcgaag cgcacctgat agccgacggt ctcgcccaca gcctcgcccc
      481 gctcctccgc cacgcgccgc gccgacatgc gcgccgccaa acgccgcggc tcaagcaccc
      541 acacctcgcc aacgcccgcc agccccgcgt cgagcatcgc gggcggcacc cgcgtcgtct
      601 tgccagcgcc cggcggcgcc tcgacgacga ggttcggcgc ctcgcggagg cgggcgagga
      661 tgtcgggcag gagcgcgtca atcggcagcg gttccataaa acgatagccg ttagccgtta
      721 gctgttagtt gttagctgtt agccatcagc gcacgggctg acggctgacg ggagcggcgc
      781 tgtcaccaat cgtcaatgca tcaagctaac agctaagagc caacagctaa cagccttttg
      841 agcacgacga gcgcgccgag gtggtaggcg ttgtgatccg cgacgagcag cgcctcgcgc
      901 aggagggtct ggccgtcgcc gtgcgggatg ggggcgtaga ggtcttgcgc ggggtctgcg
      961 accagagctt tcatcgcctt gaggtctgcg cggaagacgt cgagcgagcg ttgccacgcc
     1021 gcttcgtcgg cctcctcgcc cggcgcgggc cagtatcctt cgggccacgg cggcgaggtg
     1081 tgcgcggcgc tgcggctgaa ctcaaggatg tcccactgcg cgatgcgcat gtgctcgacg
     1141 atctgccagg cggtgtaagg gaggtgttcg accttccggt tgatcgacgc gacggtgaaa
     1201 ccggcgaccg cgctctcgaa gtcgaggtgc gcgccgccgc cgcgcagaag gtacaggagg
     1261 tgttcgcgca gagccttatc tttgtccaag tgattcttcc tccgcgtcgc cgacgtggaa
     1321 ttcgtagccc acctcgccgg acgcgacggc gcccgagccg taggtgcgct cgacgaaggt
     1381 gagcgtgccc cgccggtcga tgagcacgac ggtcgagcag cgcgtgccgt aagaagggct
     1441 gacgatgaag aggggcgaga gcagtctttc cagctcgacg ccgacgcccg tgtccggcag
     1501 ttccgcatcc gccgcgcgca cgcggtcgaa caggatgtcg atgaacgact cgggcgcgag
     1561 cgcctcgctc ccgccgacca cctcggccag agccttcttg ccccgctcga ccttcggcca
     1621 cggcgtgtcg agcaggtggt tgctcagccc gtacaccccg gggcttaacc tgtgcggggc
     1681 gccgccgcga ttcgagtagt agtaaagctc gtccgcgtcg cccgcga
//
LOCUS       DBNGOH010000183         3677 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4609175, whole genome shotgun sequence.
ACCESSION   DBNGOH010000183 DBNGOH010000000
VERSION     DBNGOH010000183.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3677)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3677)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3677
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4609175"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(45..527)
                     /locus_tag="AC159P_03445"
     CDS             complement(45..527)
                     /locus_tag="AC159P_03445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694540.1"
                     /translation="MGSEQDRIRELAERVARRLDASGPGGGGASRPSSEAAPDDLAAL
                     RAGLSEIQQRLAHIESHIAHDEDCETGAGAEGPPRRSREGHARPTGDRPSGATPARST
                     WLSGTYVPAVAHPSQEKFAGIGEAVSELVDFFEGEKTCNVEPGGKPCDHCGMCSSRGF
                     "
     gene            complement(555..851)
                     /locus_tag="AC159P_03450"
     CDS             complement(555..851)
                     /locus_tag="AC159P_03450"
                     /inference="COORDINATES: protein motif:HMM:NF015289.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HIR3694541.1"
                     /translation="MILARVLGNVVATQKNERYAGARVMVCQPVTPEGAETGATLLAL
                     DSVDAGAGDVVLIVQEGWGASTAATGEPGAAIDSAIVGVVDRIDLLEELKDVSR"
     gene            complement(848..1135)
                     /locus_tag="AC159P_03455"
     CDS             complement(848..1135)
                     /locus_tag="AC159P_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HIR3694542.1"
                     /translation="MQLARVIGTVVATMKNDSLEGRKLLVVQSLNAKLQPQGQPLVAL
                     DAVGAGLGELVFWCRGKEASFPFRREDTPTDCTVVGIVDSDAHVTSGGRAR"
     gene            complement(1270..1542)
                     /locus_tag="AC159P_03460"
     CDS             complement(1270..1542)
                     /locus_tag="AC159P_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012065534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HIR3694543.1"
                     /translation="MIIARILGTVVSTQKDERLRGSKLLLVRPLNLDGTEASGYIVAV
                     DTVGAGFHERVLVVAGSSARLAEGMKDKPVDAAIVGVIDTVDFIND"
     gene            complement(1644..1928)
                     /gene="eutM"
                     /locus_tag="AC159P_03465"
     CDS             complement(1644..1928)
                     /gene="eutM"
                     /locus_tag="AC159P_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009063953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HIR3694544.1"
                     /translation="MPQEALGMVETKGLVAMIEAADAMVKAANVTLVGYEKIGAGFVT
                     AIVRGDVAAVKAATDAGAAAARRVGELVSVHVIPRPHGSVDEALPIGADK"
     gene            complement(1986..2603)
                     /locus_tag="AC159P_03470"
     CDS             complement(1986..2603)
                     /locus_tag="AC159P_03470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694545.1"
                     /translation="MPLSSHAGNFGGRAADARAKAARLACALACCALLTTCQPEGGDL
                     VPLPRGEGQERVTTKFEGDTLRLSQRGVTMQARGRWSVADGATSVILEVSNANAESLN
                     VDFSRCEVATDGGERLTLRSAREYPGAGATSFLPDNAAVVGGGQAATFALEFKIVAED
                     GRAGVPRDMLGRTLTLRLPVESKAEPPARLDFVFDFKCAEYQRRT"
     gene            complement(2625..3131)
                     /locus_tag="AC159P_03475"
     CDS             complement(2625..3131)
                     /locus_tag="AC159P_03475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694546.1"
                     /translation="MASRLLTDAEIEFARQVFEDKLPYKKIHITSYYLPGNQGVPVTL
                     ASAGSIIATRTRRNYTIYFGPDAYNDGAHVHNAPVFIHELTHVWQGYHRKHSWEYMVE
                     SMLAQGHAIVTTGDRGGAYVYEPGGRWDGYNVEQQAHIVQDWFSNGMKTDDPLYQYIQ
                     DHIRAGRN"
     gene            complement(3197..>3677)
                     /locus_tag="AC159P_03480"
     CDS             complement(3197..>3677)
                     /locus_tag="AC159P_03480"
                     /inference="COORDINATES: protein motif:HMM:NF012804.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HIR3694547.1"
                     /translation="LTLGCVPLAEYGTPSTDELTEAMRPLVKHHNALLMANHGAVAYG
                     ADLWQAFDRLETLEHTAKIAILARALGGARNLAPDSIEKLIDVREKAGYLDERARCQS
                     CGYLHETRITCPTGERPAAAAPGAGGAAKSATNGAGKISLTREELIELLCQAVRASA"
BASE COUNT          551 a         1286 c         1180 g          660 t
ORIGIN      
        1 gtcgcttctt cctccttttc ccattttacc ctcttcgccc tctcttagaa cccgcgcgag
       61 ctgcacatgc cgcagtggtc gcagggcttg ccgccgggtt cgacgttgca ggtcttctcg
      121 ccctcgaaga agtccacgag ttcggagacg gcctcgccga tgcccgcgaa cttctcctgc
      181 gacgggtgtg cgaccgcggg gacgtaggtg ccgctcagcc acgtcgagcg cgcgggcgtc
      241 gcacctgatg gccggtcgcc cgttggccgc gcgtggcctt cgcgcgagcg acgcggcggg
      301 ccttccgcgc ccgcgcccgt ctcgcagtct tcgtcgtgcg cgatgtgcga ctcgatgtgc
      361 gcgagccgct gctgaatctc ggagagaccg gcgcgcagtg cggccagatc gtcgggcgcg
      421 gcttcgctcg acgggcggga cgcgccgccg ccgcccggcc ccgacgcgtc gagacggcgc
      481 gcgacgcgct cggccaactc gcggattctg tcctgctcgc tgcccatctg gaaacttcct
      541 tcgccccctc gccgctatcg agaaacgtcc ttcagctcct ccaacaggtc gatgcggtca
      601 acgacgccga cgatggccga atcgatggcc gcgcccggct cgcccgtcgc ggcggtcgaa
      661 gcgccccagc cctcctgcac gatcagcacc acgtcgcccg cgcccgcgtc cacagaatcg
      721 agcgccagca gcgtcgcccc cgtctccgcg ccctccggcg tcaccggctg gcacaccatc
      781 acgcgggcgc ccgcgtaacg ctcgttcttc tgcgtcgcca cgacgttgcc cagcacccgc
      841 gccagaatca tcgcgcgcgc cccccgctcg tcacgtgcgc gtccgaatcg acgatgccga
      901 cgacggtgca atcggtcggc gtgtcctcgc gccggaacgg gaagctcgcc tccttcccgc
      961 gacaccagaa gaccagctcg ccaagccccg cgccgaccgc gtcgagcgcc accagcggct
     1021 gcccctgcgg ctgcaacttc gcgttcagag actgcacgac cagaagtttg cgaccctcca
     1081 gcgaatcgtt cttcatcgtc gccaccaccg tcccgatgac ccgcgcgagt tgcataaaag
     1141 cagtaggcag caggcagcag gcagcaggca gtcgcttgag tgtaaacggt cgttggcgat
     1201 tcaactccag ccgttcggca gagggagtct ttaactgcct actgcctgct gcctgctgcc
     1261 tactgctttt caatcgttga tgaaatccac cgtgtcgatg acgccgacga tggcggcatc
     1321 gacgggcttg tccttcatgc cttcggcaag gcgcgcggac gagccggcga cgacgagcac
     1381 gcgctcgtga aagcccgcgc cgaccgtgtc cacggcgacg atgtagccgg aggcttccgt
     1441 gccgtcgagg ttcaagggcc gcacgaggag gagcttgctg ccccgcaggc gctcgtcctt
     1501 ctgcgtcgag acgaccgtcc cgaggatgcg cgcgataatc attcgcttgg taaatggtaa
     1561 atggtaaatg gtaaatggta aatggtaagc ggcggagaca gtcaacgcca ttcactattc
     1621 actattcacc attcaccgtc ggcttacttg tcggcgccga taggcagggc ttcgtcaacg
     1681 ctgccgtgcg ggcgcgggat gacgtggacg ctgacgagtt cgccgaccct gcgcgcggct
     1741 gccgccccgg cgtcggtcgc ggccttgacc gcggccacgt cgccgcggac gatggccgtg
     1801 acgaaaccgg cgccgatctt ctcgtagccg acgagcgtga cgttggccgc cttcaccatc
     1861 gcgtccgccg cctcgatcat cgccacgagg cccttcgtct ccaccattcc cagtgcttct
     1921 tgaggcattc gctgtgctcc ttcactctcc gtttcgaact cagaccttct tcgccgccct
     1981 tcgcgtcaag tccggcgctg gtattctgca cacttgaagt cgaaaacaaa atcgaggcgc
     2041 gcgggcggct ccgccttcga ctcgacgggg agccgcagcg tcaacgtgcg gcccagcata
     2101 tcccgcggca cgcccgcgcg cccgtcttcg gcgacgatct tgaactcaag cgcgaacgtc
     2161 gccgcctgcc cgccgccgac gacagcggcg ttgtcgggta agaatgacgt ggcgcccgcg
     2221 ccgggatatt cgcgcgcgga gcgcaaggtc aggcgctcgc cgccgtccgt cgccacctcg
     2281 caacggctga agtctacgtt caggctttcg gcgttggcgt tgctgacctc aagtataacg
     2341 gaagtcgcgc cgtcggcgac actccaacgg ccccgcgcct gcatcgtcac gccgcgctgc
     2401 gagagtcgca acgtgtcgcc ttcgaacttc gtcgtgacgc gctcctggcc ttcgccgcga
     2461 gggagcggca cgaggtcgcc gccttcgggc tggcacgtcg tcaggagcgc gcagcaggcg
     2521 agcgcgcagg cgagccgcgc cgccttcgca cgcgcgtcgg cggcgcggcc tccgaagttt
     2581 cccgcgtgtg acgatagagg cataagaccg ccggtgcgtc gcgttcagtt ccgaccggcg
     2641 cggatatgat cctgaatgta ttggtacagc gggtcgtctg tcttcatccc gttgctgaac
     2701 cagtcctgca cgatgtgcgc ctgctgctcg acgttgtagc cgtcccagcg cccgcccggc
     2761 tcgtagacgt acgcgccgcc gcggtcgccc gtcgtgacga tggcgtgccc ctgcgcgagc
     2821 atcgactcga ccatgtactc ccaggagtgt ttgcggtggt agccctgcca gacgtgcgtc
     2881 agctcgtgga tgaagaccgg ggcgttgtgc acgtgcgcgc catcgttgta agcgtccggc
     2941 ccgaagtaga tcgtgtagtt cctgcgcgtg cgcgtggcga tgatcgagcc agccgaagcg
     3001 agcgtcaccg ggacgccctg attgcccggc aggtaataac ttgtgatgtg gattttcttg
     3061 taaggcagct tgtcctcgaa gacctggcgt gcgaattcta tctccgcgtc ggtgagcagg
     3121 cgactagcca tgtcggggcc tccttagccg tggtgagatg gactgtacgg aacgaggggc
     3181 cgcggtcttc ggaacttcac gcgctcgcgc gcacggcctg gcacagcagc tcgatcaact
     3241 cctcgcgcgt gagcgagatt ttacccgcgc cgttggtcgc ggacttcgcc gcgccgcccg
     3301 cgccgggtgc cgccgccgcc gggcgctcgc cggtcgggca ggtgatgcgc gtctcgtgca
     3361 ggtagccgca cgactggcag cgcgcgcgct cgtcgaggta gcccgccttt tcgcggacgt
     3421 cgatcaattt ttcgatcgag tcgggcgcga ggttgcgtgc gccgccgagc gcgcgcgcga
     3481 ggatggcgat cttcgccgtg tgctccaggg tttcgaggcg gtcgaaagcc tgccacaaat
     3541 ccgcgccgta ggcgaccgcg ccgtggttcg ccatcagcag cgcgttgtga tgcttgacga
     3601 gcgggcgcat cgcctctgtc aactcgtcgg tcgagggcgt gccgtactcg gcgagcggga
     3661 cgcagcccag cgtcagt
//
LOCUS       DBNGOH010000184         2737 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_26774, whole genome shotgun sequence.
ACCESSION   DBNGOH010000184 DBNGOH010000000
VERSION     DBNGOH010000184.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2737)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2737)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2737
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_26774"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..660)
                     /locus_tag="AC159P_03485"
     CDS             complement(<1..660)
                     /locus_tag="AC159P_03485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694548.1"
                     /translation="MAKKKYQVVGRVVSASYPERGVASARVEAWDKDLLVDDLVGSAV
                     TGADGGFRIEFDTNYFLELFLERKPDLFFKVYYYDELVKSTEDSVLWNIEAGETEITI
                     EVNVATELKGDVYMPKKPGKSGKGGGKYGAGKPTTGSKYGKGSKPPGGAKYGGTKYGG
                     SKKDDGDKGKKKHPYPDGSSFVGVIISRACAIELLTALSLALGFPPSKKKKNKKGKKD
                     KN"
     gene            complement(710..>2737)
                     /locus_tag="AC159P_03490"
     CDS             complement(710..>2737)
                     /locus_tag="AC159P_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016394799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694549.1"
                     /translation="VSTGGQPPPGTQTVNYYNAPLPWRTQGNCDLSYAFSSSCKPVET
                     PGFGPNGDPGTPIMRAYENDKVQVRTLVGAHTFSHFFTMAGPRWLFEPSWSNSGYRSS
                     QGMGLSEHFEMLFNVPASSQAAAPAASRRKCPDKSPEGTSQGNCVDYLYSPSEDDFGL
                     TNGVWGLLRAYDPTKTFKQLAPLPNNPLTAASMQNKTLPFAGCPANLPASKMKKYNIS
                     AVTAKTALTPQQLILFNARGTQTGGTLIQSLGMMYVFTEDLDAGGKLKGPVEPLILRA
                     NAGDCIDITLTNSLDTTAPPFTTPLPWPKPFDVVLPGIPTNVTASKYVGLHPQLLSYD
                     GATDGGLNVGWNAKPQTAAPGAKVSYRWYAGSINRNANGTLAYTPIEFGSLNLMPSDP
                     FMQHLWGLYGGMVIEPANAQWKCDAEDAGGNKIQVPCEPASGFDPSTVKNYTRASATV
                     STATGTTLFREFVAMTSEDLKLNDSNRSAVNYRVEPTYFRYGNTNGAAPKFAADGNNS
                     CALANVLVGGDPQTPIFTATAGTPFRFRLLHPQGTGTAQVFALSGHVWQREPYTNNST
                     VIGNNPLSQWMGSHDSYGGTDHFDLVGAMAGGQNQVPGDYLYTTFVPNQNLFGSWGLF
                     RVLDKNGNQVKGTQVAGSRPNSCPTVPGSQYGAGRPNENIQRFVRPPFGTQ"
BASE COUNT          381 a          853 c          956 g          547 t
ORIGIN      
        1 gtttttgtcc ttcttgccct tcttgttctt cttcttcttc gacggtggga agccgagagc
       61 cagactgagc gcggttaaaa gctcgatggc gcaggcccgg ctgatgatca cgccgacgaa
      121 cgaggagccg tccgggtacg gatgcttctt cttgcctttg tctccgtcgt ccttcttgct
      181 gcccccgtac ttcgtgccgc cgtacttggc gccgccgggg ggcttgctgc ctttgccgta
      241 cttgctgccg gtggtgggct tgccggcgcc gtactttcca ccgccttttc cactcttgcc
      301 tggtttcttc ggcatgtaga catctccttt cagctcggtc gcgacgttca cttctatggt
      361 gatctcggtc tctccggcct cgatgttcca caggactgaa tcttccgtgc tcttaaccag
      421 ctcgtcgtag tagtagacct tgaagaagag gtcgggcttg cgctccagaa agagttcgag
      481 gaagtagttc gtgtcgaact cgatccggaa gccgccgtcc gcccccgtga ccgcgctgcc
      541 gaccaggtca tcaacgagca ggtctttatc ccaggcctcg acgcgcgcgc tcgccacgcc
      601 ccgctccggg tagctggcgc tgaccacgcg accgacgacc tgatacttct ttttcgccat
      661 ggctatcctc cctctggccg aaacccctca gggccgagcg cgcgaccggc tattgcgtgc
      721 cgaacggcgg cctgacgaag cgctggatgt tctcgttggg ccgcccggcg ccgtactggc
      781 tgccgggaac ggtcgggcac gagttgggtc tggagccggc cacctgagtg cccttcacct
      841 ggttgccgtt cttgtcgagc acgcggaaga ggccccacga gccgaacagg ttctggttgg
      901 gaacgaaggt ggtgtacagg tagtcgccgg ggacttggtt ctggccgccg gccatcgccc
      961 cgacgaggtc gaagtgatcg gtgccgccgt agctgtcgtg cgaccccatc cactgcgaga
     1021 gcgggttgtt gccgatcacg gtcgagttgt tggtgtacgg ctcgcgctgc cagacgtggc
     1081 cgctgagggc gaagacctgc gccgtgccgg tgccctgcgg gtgcagcagg cggaagcgga
     1141 agggcgtgcc cgcggtcgcc gtgaagatgg gcgtctgcgg gtcgccgccc accagcacgt
     1201 tcgccagcgc gcaactgttg ttgccgtcgg cggcgaactt cggcgcggcc ccgttggtgt
     1261 tgccgtagcg gaagtaggtc ggctcgacgc ggtagttgac cgcgctccgg ttgctgtcgt
     1321 tgagcttcaa gtcctcgctg gtcatcgcca cgaactcgcg gaagagggtc gtgccggtgg
     1381 ctgtggagac ggtcgccgag gcgcgcgtgt agttcttcac ggtggacggg tcgaagccgg
     1441 acgcaggctc gcacggaacc tgaatcttgt tcccgcccgc gtcttccgcg tcgcacttcc
     1501 actgcgcgtt cgcaggctcg atgaccatgc cgccgtagag accccagagg tgctgcatga
     1561 aggggtcgga cggcatcagg ttgagcgagc cgaactcgat cggcgtgtag gcgagcgtcc
     1621 cgttcgcgtt gcggttgatg ctgcccgcgt accagcggta gctgaccttc gcgccggggg
     1681 cggcggtctg cggcttcgcg ttccagccga cgttgaggcc gccgtcggta gcgccgtcgt
     1741 acgagagcag ttgcgggtgc agcccgacgt atttcgacgc ggtgacattg gtcgggatgc
     1801 cggggaggac gacatcgaac ggcttcggcc acggcaaggg ggtggtgaac ggcggcgccg
     1861 tcgtgtcgag cgagttggtc agcgtgatgt cgatgcagtc gcccgcgttg gcccgcagga
     1921 tgagcggctc gaccggcccc ttcagtttcc cgccggcgtc gaggtcttcc gtgaagacgt
     1981 acatcatgcc gagcgactgg atgagcgtgc cgccggtctg cgtgccgcgc gcgttgaaga
     2041 ggatcaactg ctgcggcgtg agcgccgtct tcgcggtcac cgccgagatg ttgtacttct
     2101 tcatcttgct cgccggcagg ttcgccgggc agcccgcgaa gggcagcgtc ttgttctgca
     2161 tcgacgcggc ggtcagcggg ttgttcggca gcggcgcgag ctgcttgaac gtcttggtcg
     2221 ggtcgtaggc gcggagcaac ccccacacgc cgttggtcag gccgaagtcg tcctcgctcg
     2281 ggctgtagag atagtccacg cagttgccct gcgacgtccc ctcggggctt ttgtcggggc
     2341 acttgcgccg cgacgccgcg ggcgcggcgg cctgcgaaga ggccggcacg ttgaagagca
     2401 tctcgaagtg ctcggagagg cccatgccct gactgctgcg gtagccggag ttcgaccacg
     2461 aaggctcgaa cagccagcgc gggccggcca tcgtgaagaa gtgcgagaag gtgtgcgcgc
     2521 cgaccagcgt gcgcacctgc accttgtcgt tctcgtaggc gcgcatgatg ggcgtgccgg
     2581 ggtcgccgtt cggcccgaag ccgggggtct cgaccggctt gcaggaggag gagaaggcgt
     2641 acgagaggtc gcagttgccc tgcgtccgcc acgggagcgg cgcgttgtag tagttgacgg
     2701 tctgcgtccc cggcggcggc tggccgcccg tgctgac
//
LOCUS       DBNGOH010000185         3531 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4792558, whole genome shotgun sequence.
ACCESSION   DBNGOH010000185 DBNGOH010000000
VERSION     DBNGOH010000185.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3531)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3531)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3531
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4792558"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..600)
                     /locus_tag="AC159P_03495"
     CDS             complement(<1..600)
                     /locus_tag="AC159P_03495"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013134.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HIR3694550.1"
                     /translation="MRANVPGYELAVPRDLGEALRLLAEGPGAWRPFAGGTDLMVLFE
                     AGRLAHKKFFSIRHLAELRGIEESEAHVTLGALTTYTDVRRSETLRREFPMLCAAARE
                     TGGLAIQNRGTVGGNIANASPAADTPPALLAYGAEVELVSTAGARWVAYEEFHTGYKQ
                     TLMRADELLARVRVRRPRGGAVQHYRKVGTRRAQAISKVC"
     gene            complement(597..1100)
                     /locus_tag="AC159P_03500"
     CDS             complement(597..1100)
                     /locus_tag="AC159P_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HIR3694551.1"
                     /translation="MKSSVSDIPRKVEIDCTINGERVSLRAYPMVRLLDVLREELRLT
                     GTKEGCGEGECGACTVLVDGEMVNSCLVPFAQVAGADIKTIEGVADGERLHAVQQAFV
                     ECGGAQCGICTPGMVIAAVSLLERERRPTEADIREGLAGNLCRCTGYMKIFEAVVRAC
                     ENTGGDA"
     gene            complement(1115..3349)
                     /locus_tag="AC159P_03505"
     CDS             complement(1115..3349)
                     /locus_tag="AC159P_03505"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HIR3694552.1"
                     /translation="MSEKLIGRSVPRKEGRDKVTGRARYVDDITLPGMLHGVTVRSPA
                     PRGRVRDIVFGEHIPWDEFVVVTAKDIPGANSVALILNDQPYLADGVVNHPEEPIVLL
                     AHEDKYLLEEARRAVRFEIEPLPAVFTIEDALDQKEIVWGSDNIFKSYLVDKGNVDDV
                     WGSADFVVEGEYRTGAQEQLYIETNGIIAVANKEEGVTVRGSMQCPYYVHKALMALFD
                     LPEDKVRVVQTETGGGFGGKEEYPSMIAGHAALLAWKSGRPVKIVYDRAEDMAATTKR
                     HPSRTRHRTAVSREGKLLGMEIDFVVDGGAYATLSAVVLSRGTIHAAGPYNCPNVRIR
                     SRAVATHAPPHGAFRGFGAPQSIFALERHLDKVAATIGLAPAELRRRNFIREGETTAT
                     EQVIREPVEMDALLERAFELSDYRARRERFARENVGSAVKRGIGFAAFMHGAGFTGSG
                     EKYLQSIVGCEATSEGRVRVLAASTEIGQGTNTIFAQIAAEALGIDYEQIETAQPDTG
                     HVPNSGPTVASRTCMIVGKLVESAALGLKQTLVGQGLLTDTYTSEEFAAACRAYVERH
                     GALRSFSQYRQPDNIEWDDGKYRGDAYGAYAWAVYVAEVSVDTVTYEARVTDFVAVQE
                     VGRVIHPVLAAGQIEGGVAQGIGYALYETVVWQDGRMANGQMTNYIMPTSADLPPIRV
                     HFEEVPNALGPGGAKGIGELPMDGPAPAIVNAVEHATGVAFNHIPLMPEAIMEALAAE
                     AVGA"
BASE COUNT          497 a         1195 c         1226 g          613 t
ORIGIN      
        1 gcagaccttc gagatggcct gcgcgcggcg cgtcccgacc ttgcggtagt gctggaccgc
       61 gcccccgcgc gggcgccgca cgcggacgcg ggcgagcagc tcgtccgcgc gcatcagggt
      121 ctgtttgtag ccggtgtgga actcttcgta ggcgacccag cgcgcgccag ccgtcgagac
      181 cagctcgacc tccgcgccgt aggcgaggag cgcgggcggc gtgtcggcgg cggggctggc
      241 gttggcgatg ttgccgccga cggtgccgcg gttctggatg gcgaggccgc ccgtctcgcg
      301 cgccgccgcg cacagcatcg ggaactcgcg ccgcagcgtc tcgctccgcc gcacgtcggt
      361 gtaagtcgtc agcgccccga gcgtgacgtg tgcttccgac tcttcgatcc cgcgcagctc
      421 cgcgaggtgc cggatgctga agaacttctt atgcgcgagc ctccccgcct cgaatagcac
      481 catcaagtcc gtcccgcccg cgaacggtcg ccacgcgccc ggcccttcgg cgagcagccg
      541 cagcgcctcg ccgaggtcgc gcggcacggc cagctcgtaa ccggggacgt tggccctcac
      601 gcgtcgcccc ccgtgttctc gcacgcgcgc acgaccgcct cgaagatttt catgtagccc
      661 gtgcagcggc agaggttccc cgcgaggccc tcgcggatgt ccgcttcggt cgggcgccgc
      721 tcgcgttcga gcaggctgac ggcggcgatc accatgcccg gcgtgcagat gccgcactgc
      781 gcgccgccgc actcgacgaa agcctgctgg acggcgtgca ggcgctcgcc gtcggcgacg
      841 ccctcgatgg tcttgatatc cgcccccgca acttgcgcga acggcacgag acaactgttg
      901 accatctcgc cgtccaccag caccgtgcac gcgccgcact cgccctcgcc gcagccctcc
      961 ttcgtgccgg tcaggcgcag ctcctcgcgc agcacgtcga gcagccgcac catcgggtag
     1021 gcgcgcaagc tgacgcgctc gccgttgatg gtgcagtcga tctcgacctt gcgggggatg
     1081 tcggacacgc tactcttcat cgatcatcgt tcgctcaagc tccgaccgcc tcggcggcga
     1141 gcgcctccat gatggcttcg ggcatcaggg ggatgtggtt gaaggcgacg ccggtcgcgt
     1201 gctcgacggc gttcacgatg gcgggcgcgg ggccgtccat cggcagctcg ccgatgccct
     1261 tcgcgccgcc ggggccgagc gcgttgggca cctcctcgaa gtggacgcgg atcggcggca
     1321 ggtctgccga ggtcggcatg atgtagttgg tcatctggcc gttcgccatc cgcccgtcct
     1381 gccacacgac cgtctcgtag agcgcgtagc cgatgccttg cgcgacgccg ccctcgatct
     1441 gccctgcggc gagcacggga tggatcacgc gccccacctc ctgcacggcg acgaaatcgg
     1501 tgacgcgcgc ctcgtaagtc accgtgtcaa cgctcacctc cgcgacgtag acggcccacg
     1561 cgtacgcgcc gtaggcgtcg ccgcggtact tgccgtcgtc ccactcgatg ttgtcgggct
     1621 gccggtactg gctgaacgac ctgagcgcgc cgtgccgctc gacgtaagcg cggcacgcgg
     1681 cggcgaactc ttccgaagtg taagtgtccg tgagcagccc ttggccgacg agcgtctgct
     1741 tgaggccgag cgcggcggat tcgacgagct tgccgacgat catgcaggtg cgcgaggcga
     1801 ccgtggggcc gctgttcggg acgtggccgg tgtccggctg cgccgtctcg atctgctcgt
     1861 agtcgatgcc gagcgcctct gccgcgatct gcgcgaagat ggtgttggtg ccctgcccga
     1921 tctctgtgct cgcggcgagc acgcgcacgc ggccctcgct cgtcgcctcg cacccgacga
     1981 tcgattgcag atacttttcg cccgacccgg tgaagcccgc gccgtgcatg aaggcggcga
     2041 agccgatgcc gcgcttgacg gcgctcccga cattttcgcg ggcgaaccgc tcgcgcctgg
     2101 cgcggtagtc ggacagctcg aacgcgcgct ccaacagcgc gtccatctcc acgggttcgc
     2161 ggatgacctg ctcggtggcg gtcgtctcgc cctcgcggat gaagttgcgg cggcgcagct
     2221 ctgcgggcgc gaggccgatg gtcgcggcga ctttatcgag gtggcgttcg agggcgaaga
     2281 tgctctgcgg cgcgccgaag ccgcggaagg cgccgtgcgg cggcgcgtgg gtggcgacgg
     2341 cgcgcgagcg gatgcggacg ttggggcagt tgtacggccc cgccgcgtgg atcgtgccgc
     2401 gcgagaggac gaccgccgag agcgtggcgt aggcgccgcc atccacgacg aagtcgatct
     2461 ccatcccgag cagtttgcct tcgcgcgaga cggcggtgcg gtggcgcgtg cgcgaggggt
     2521 ggcgcttggt ggtcgccgcc atgtcttcgg cgcggtcgta gacgatcttc acggggcggc
     2581 ccgacttcca agccagcagc gccgcgtggc ccgcgatcat cgaggggtac tcttccttgc
     2641 cgccaaagcc gccgcccgtc tcggtctgga cgacgcggac tttgtcttcc ggcagatcga
     2701 acaaagccat cagcgctttg tgcacgtagt aagggcattg catcgagccg cggacggtga
     2761 cgccctcttc cttgttagcg acggcgatga tgccgttcgt ctcgatgtag agctgctcct
     2821 gcgcgcccgt ccggtactcg ccctcgacga cgaagtcggc gctgccccac acgtcgtcaa
     2881 cgtttccttt atcgacgaga tacgacttga aaatattatc gctgccccag acgatctctt
     2941 tttggtcgag cgcgtcttcg atggtgaaga cggcgggcag aggctcgatc tcgaagcgga
     3001 cggcgcggcg ggcctcttcg agcaggtact tgtcttcgtg cgcgagcagg acgatcggct
     3061 cttcggggtg gttgacgacg ccgtcggcga ggtagggctg gtcgttgagg atgagggcga
     3121 cgctgttcgc gccggggatg tccttcgcgg tgacgacgac gaactcgtcc cacgggatgt
     3181 gttcgccgaa gacgatgtcg cggacgcgac cgcgcggcgc ggggcttctg acggtcacgc
     3241 cgtggagcat gccggggagc gtgatgtcgt ccacgtagcg cgcgcggccc gtgaccttgt
     3301 cgcggccttc cttgcggggg acagagcgcc cgattagctt ctcactcatc tgcggctttc
     3361 ctgaaggctc gaacggcggc tgccggcgtg cgggagagtg taacacggaa tgcggaatgc
     3421 ggatttcgga ttgcgggtta aaaaaccggt gacgagcgat gaaagcgatg ctgtcatcag
     3481 tcgtcaccgg tttcaagacg cggtcgtaac tcttccgatc cgcattccgc a
//
LOCUS       DBNGOH010000186         7079 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1310959, whole genome shotgun sequence.
ACCESSION   DBNGOH010000186 DBNGOH010000000
VERSION     DBNGOH010000186.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7079)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7079)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7079
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1310959"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..596)
                     /locus_tag="AC159P_03510"
     CDS             complement(<1..596)
                     /locus_tag="AC159P_03510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694553.1"
                     /translation="MHSSILERSLMKAACASLLVAACAVSASAQLGGLGGVTDKLKKK
                     TPSLLGGKEPITTSLPDAKWGDPAKDGFTPREAVRSLLQLQRTFNGGFVLQPGYYATQ
                     TQSYCLKAGTHGPGAGDGYLYAPPKGPAEDAVMTIVRNSVNRPEIPQQQIQQLLWAII
                     ARAKFEDLTSQLKATASQLLTPRQLASLNRSALDALSG"
     gene            complement(701..1120)
                     /locus_tag="AC159P_03515"
     CDS             complement(701..1120)
                     /locus_tag="AC159P_03515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694554.1"
                     /translation="MLVPLVVVILLACSAGVAQERPHTPGQGNPERKAILDALRAPVE
                     KRLKRKVVFKVDELKVLDGWAFVRGVPQQPGGKAMDYSGTPYQQQRDDGAFDDGFSAL
                     LRAKGGKWSVVTYNIGATDVVWADWAEKYKAPPEIFN"
     gene            1475..2593
                     /gene="gcvT"
                     /locus_tag="AC159P_03520"
     CDS             1475..2593
                     /gene="gcvT"
                     /locus_tag="AC159P_03520"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HIR3694555.1"
                     /translation="MTAPSLKQTPLNAAHRRMGGRMVDFGGWDMPVQYPAGTVEEHNR
                     TRTRAGLFDVSHMGEIEARGRDAVRFVNRLCSNDVTKLVDGQAHYSALTTPQGTVVDD
                     LLVYRFGPEHLLLVVNASTTDKDWDWITSHKQDEAVELVNRSADFCQIALQGPEVLRI
                     LQTLTETPLEEIKYYHFREGAVDGVPSIISRTGYTGEDGFEVYAPADKAEHLWEGMLA
                     AGGYGTPEGVLPCGLAARNTLRLESAMSLYGHEIDEEHTLLEANLGWICKLGKGDFIG
                     REALQRQKEEGVRRKLVGFEVTDRGIARDGQDVHIDGAKVGIVTSGSPAPYLKKNIGL
                     AYVPAEHASVGQQLQIDVRGRLVGAEVVATPFYKREKK"
     gene            2732..3121
                     /gene="gcvH"
                     /locus_tag="AC159P_03525"
     CDS             2732..3121
                     /gene="gcvH"
                     /locus_tag="AC159P_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HIR3694556.1"
                     /translation="MANVPENLQYSKDHEWLRVEGDTGTVGITDHAQNSLGDVVYVEL
                     PKVGETFAAHGTFGSVESVKAVSELYLPVGGEVTEVNESLQDEPEKVNTDPYGEGWMV
                     RVRLADKGEVDGLLTAAEYEDYLKSEE"
     gene            3132..4493
                     /gene="gcvPA"
                     /locus_tag="AC159P_03530"
     CDS             3132..4493
                     /gene="gcvPA"
                     /locus_tag="AC159P_03530"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HIR3694557.1"
                     /translation="MRYIPNSPEERQEMLQTVGLGSAAELFDSIPADVLLRAPMNVPE
                     ALSEVELLARFEEMAARNTAARRPSFLGAGAYSHYAPTVIDSLLQRSEFFTAYTPYQP
                     EISQGTLQAIYEFQTLVCQLTGMDVANASMYDGSTAMAEAVLMAERVTRRSRVLVSAA
                     VHPDYLHVAETYVAHAGIELRQLGFEGETGRTALGDDEALDKSVAAVVVQSPNFFGCV
                     EDLKALAEEVHAAGALLVVVVTEAASFGLLRSPGACGADIVVAEGQSLGVPVSYGGPY
                     VGLFATREKYARQIPGRLAGIAFDAQGRRGFVLTLATREQHIRREKATSNICTNEGLI
                     ALAATIYMTTMGRRGLQEVAAQCAQKAAYARRRIAALEGFFLPHTAPVFNEFVVRAPS
                     DAGELLRRLAAEKGITGGLALSRYSNDRPNDFLVCVTETNTRAEIDALVAGLEHVATS
                     IPF"
     gene            4501..6036
                     /gene="gcvPB"
                     /locus_tag="AC159P_03535"
     CDS             4501..6036
                     /gene="gcvPB"
                     /locus_tag="AC159P_03535"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HIR3694558.1"
                     /translation="MSSHDITKAAPHITQNEALVFERSHPGRVGFMLSPLDVEETPID
                     ELLPAELRRDDDLEGVPELSEVDVVRHFTRISTWNYNIDLGMYPLGSCTMKYNSRLNE
                     KVARIAGFANLHPLAPEEDAQGALEVMHELQQHLAEITGLPGISLQPAAGAHGEMTGV
                     MMIRAFLDARDGVGEGDQPRRNVMLIPDSAHGTNPASAHLSGFTVKTIRSTNEGLTDL
                     GHLRELCASGDVAGLMLTNPNTVGIFERNIQEICRIIHDAGGLVYMDGANMNALVGVA
                     RPGDMGVDVIHLNLHKTFSTPHGGGGPGSGPCCCTAELEPFLPIPRVVREGERFRLTY
                     DRPQSIGRVKAFYGNFGMHLRALAYTLTHGRTGLREATEAAVLNARFVAHGLADTYDK
                     PFDSPPMHEVVFTDKRQSRKGVHTLDIAKRLIDYGFHPMTIYFPLIVQGAMLIEPTES
                     VGRQELQQFIDAMKQIAREALEEPDTVLKAPHTTRIGRLDEAAAARKPVLRWRPAEKT
                     ATA"
     gene            complement(6123..6711)
                     /locus_tag="AC159P_03540"
                     /pseudo
     CDS             complement(6123..6711)
                     /locus_tag="AC159P_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895341.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase N-terminal SAM-like
                     domain-containing protein"
     gene            6855..>7079
                     /locus_tag="AC159P_03545"
     CDS             6855..>7079
                     /locus_tag="AC159P_03545"
                     /inference="COORDINATES: protein motif:HMM:NF025006.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HIR3694559.1"
                     /translation="MKDSKHFILLLCCLAVLVPLCEFSVGSQHTCGGIEGRVTTEDGW
                     VIPGANIRLMNKRTKQSTRVQTNGEGEYTIC"
BASE COUNT         1283 a         2360 c         2259 g         1177 t
ORIGIN      
        1 ttccccgaga gcgcgtcgag cgcgctgcgg ttgagcgagg cgagctggcg cggcgtcaag
       61 agctgcgagg ccgtcgcctt cagttgcgac gtgaggtctt cgaacttggc gcgcgcgatg
      121 atcgcccaga ggagctgctg aatctgctgc tgcggaatct cggggcggtt caccgagttg
      181 cggacgatgg tcatcacggc gtcttccgcc ggccctttgg gcggcgcgta gaggtagccg
      241 tcgcccgcgc cggggccgtg cgtgccggcc ttcaggcagt agctctgcgt ctgcgttgcg
      301 tagtagccgg gctggaggac gaagccgccg ttgaaggtgc gctggagctg gagcagagac
      361 cgcacggcct cgcgcggcgt gaagccgtcc tttgccgggt cgccccactt cgcgtccggc
      421 aggctcgtcg tgatcggctc cttgccgccg agcaggctcg gcgtcttctt cttcagcttg
      481 tcggtgacgc ccccgaggcc gcccaactgc gccgacgccg aaaccgcgca cgccgcgacc
      541 aacaaagagg cgcacgccgc cttcatcaaa gaacgttcaa gaatcgagct gtgcattgaa
      601 attcctcccg agggtggaaa gaaaatcgaa atggactcct acgccgacga ctccgacgac
      661 gattagcgcg ggtcgcgctg cccgcggcgc gtgaacgcgg tcagttgaaa atctctggcg
      721 gcgccttgta tttttcggcc cagtcggccc agaccacgtc ggtcgcgccg atgttgtagg
      781 tgacgaccga ccacttgccg cccttcgcgc ggagcagggc gctgaagccg tcgtcgaagg
      841 cgccgtcgtc gcgctgctgc tggtagggcg tgccgctgta gtccatggcc ttaccgccgg
      901 gctgctgcgg cacgccgcgc acgaaggccc acccgtcgag caccttcagc tcgtccacct
      961 tgaagacgac cttgcgcttg agccgcttct cgacgggcgc gcgcagcgcg tcgaggatgg
     1021 ccttccgctc cgggttcccc tgccccggcg tgtgcgggcg ctcctgcgcg acgcccgccg
     1081 agcaggccaa gaggataacg acgacgagtg gcaccagcat ttttcgcaac aatgaactcc
     1141 acctccgaga cgagagatta tagcaaagta ggcggtaggc agttgaaaca gaatacagaa
     1201 gtcggaagtc ggaagtcgga agtcggaaag acgcaggggc ttgggaggta aagtgccaag
     1261 cctctgcgtg cgacaggcag taaagtgaag aggcttggga tggaacgtcc caagcctctg
     1321 cgtctttccg acttccgact tctgttttaa ctgcctaccg cctacttttc cccttgcttg
     1381 acctcgtctc gcgcggcgtg ttaggttttc gcatcgtcaa gactaacatg ccgaaggccc
     1441 ttatttcgca gtacagacag cgcaggagac gcgtgtgacc gctccctctt tgaagcagac
     1501 gccgctcaac gcagcgcacc ggcggatggg cgggcgcatg gtcgatttcg gcgggtggga
     1561 catgcccgtc cagtaccccg ccggcaccgt cgaggagcac aaccggacgc gcacccgcgc
     1621 cggcctgttc gacgtgtcgc acatgggcga gatcgaggcg cgcgggcgcg acgccgtccg
     1681 gttcgtcaac cgcctctgct cgaacgacgt gacgaagctc gtggacgggc aggcgcacta
     1741 ctccgcgctc accaccccgc agggcaccgt cgtcgatgac ctgctcgtct accgcttcgg
     1801 ccccgaacac ctcctgctcg tcgtcaacgc ctcgaccacc gacaaggact gggactggat
     1861 cacctcgcac aagcaggacg aagcggtcga actcgtcaac cgcagcgcgg acttctgcca
     1921 gatcgctctg caaggccccg aagtcctgcg catcctgcaa acactgaccg agacgccgct
     1981 cgaagagatc aagtattacc acttccgaga gggcgcggtg gacggcgtgc cctcgatcat
     2041 ctcgcgcaca ggctacacgg gcgaggacgg cttcgaggtc tacgcccctg cggacaaggc
     2101 cgagcacctg tgggagggca tgctggcggc gggcggctac ggcacgcccg aaggcgtcct
     2161 gccctgcggg ctggccgcgc gcaacacgct ccggctcgaa tccgcgatgt cgctctacgg
     2221 ccacgagatc gacgaagagc acacgctcct cgaagccaac ctcggctgga tttgcaaact
     2281 gggtaagggc gacttcatcg ggcgcgaggc gctacagaga cagaaggagg agggcgtccg
     2341 gcgcaagctc gtcggcttcg aagtcaccga ccgcggcatc gcccgcgacg gccaggacgt
     2401 gcacatcgac ggcgcgaagg tcggcatcgt cacctccggc agccccgcgc cctatctcaa
     2461 aaagaacatc ggcctcgcct acgtccccgc cgaacacgcg agcgtgggcc aacagcttca
     2521 gatcgacgtg cgcgggcgac tcgtcggcgc cgaagtggtg gcgactccat tctacaagag
     2581 ggagaagaag tgaaagagct gttggctgtc agcgattagc tattagcttt tttgtctctg
     2641 gcgaaagctg agcggcgagc cgaaccgccc cactcgtgca agagaattgt agctaacagc
     2701 taatcgctaa cagcttcact taaggagaat catggcaaac gttcctgaga atctacagta
     2761 cagcaaggat cacgagtggc tgcgggtcga gggcgacacg ggcaccgtgg ggattacaga
     2821 ccacgcgcag aactctctgg gcgacgtggt ctacgtcgag ctgccgaagg tgggcgagac
     2881 gttcgcggcg cacggcacgt tcggctcggt cgagtcggtg aaggccgtct cggagctgta
     2941 cctgcccgtg ggcggcgagg tcacggaggt caacgagtct ttgcaggacg agccggagaa
     3001 ggtcaacacg gacccctacg gcgagggctg gatggtgcgc gtccggctcg ccgacaaggg
     3061 cgaggtggac ggcctgctca ccgccgccga gtacgaggac tatctgaagt ccgaggagta
     3121 ggtaaaccgc cgtgcgctac atcccaaact cgcccgaaga gcggcaggag atgctgcaaa
     3181 cggtcggcct cggctcggcg gcggaactct tcgactcgat ccccgcggac gttctgctgc
     3241 gcgccccgat gaacgtcccc gaggccctgt cggaggtcga gctgctcgcg cgcttcgaag
     3301 agatggccgc gcgcaacacg gcggcccgcc gcccgagctt cctcggcgcg ggcgcctact
     3361 cgcactacgc gccgacggtc atcgacagcc tgctccagcg ctccgagttc ttcaccgcct
     3421 acacgcccta ccagccggaa atttcgcagg gcacgctgca agccatctac gagttccaga
     3481 cactggtctg ccagttgacg gggatggacg tggccaacgc gtcgatgtac gacggctcga
     3541 ccgcgatggc cgaggccgtg ctcatggccg agcgcgtcac gcgccgctcg cgcgtgctcg
     3601 tctcagccgc cgtgcacccg gactacctgc acgtcgccga aacctacgtc gcgcacgccg
     3661 gcatcgagct gcggcagctc ggcttcgagg gggagacggg gcgcacggcg ctcggcgacg
     3721 acgaggcact cgacaagtcg gtcgcggcgg tcgtcgtcca gtcgccgaac ttcttcggct
     3781 gcgtcgaaga cctcaaagcg ctggccgaag aggttcacgc ggcgggcgcg ctgctcgtcg
     3841 tcgtcgtcac agaggccgct tcgttcgggc tgctgcgctc gccgggcgcg tgcggcgcgg
     3901 acatcgtcgt ggccgaaggc cagtcgcttg gcgtgcccgt ctcgtacggc ggcccctacg
     3961 tcgggctgtt cgccacgcgc gagaagtacg cgcggcagat accgggccgg ctcgcgggca
     4021 tcgccttcga cgcgcaagga aggcgcggct tcgtcctgac gctcgccacg cgcgagcagc
     4081 acatccgccg cgagaaggcg acctcgaaca tctgcaccaa cgaggggctg atcgcgctgg
     4141 ccgcgaccat ctacatgacg acgatgggcc gccgtggctt gcaggaggtg gccgcgcagt
     4201 gcgcgcagaa ggcggcctac gcgcgccgcc gcatcgccgc gctcgaaggc tttttcctcc
     4261 cccacacggc gcccgtcttc aacgagttcg tcgtgcgcgc gccttcggac gcgggcgagt
     4321 tgctgcgccg tctcgccgcc gaaaaaggca tcaccggcgg cctcgcgctc tcgcgttatt
     4381 cgaacgaccg ccccaacgac ttcctcgtct gcgtcaccga gacgaacacg cgcgccgaga
     4441 tcgacgcact cgtcgccgga cttgaacacg tcgcaacttc catccctttt taagcgcaag
     4501 atgagttcac acgacatcac caaagccgcg ccgcacatca cgcagaacga agcgctcgtc
     4561 ttcgagcgct cgcaccccgg cagggtcggc ttcatgctgt ccccgctcga tgtggaagag
     4621 acgccgatcg acgagctgct gcccgccgaa cttaggcgcg acgacgacct cgaaggcgtg
     4681 cccgaactct ccgaggtgga cgtggtgcgc cacttcacgc gcatctcgac gtggaactac
     4741 aacatcgacc tcggcatgta cccgctcggc tcatgcacga tgaagtacaa ctcgcgtctc
     4801 aacgagaagg ttgcgcgcat cgcgggcttc gccaacttgc acccgctcgc gcccgaagag
     4861 gacgcgcagg gcgcgctcga agtgatgcac gagttgcagc agcacctcgc ggagatcacc
     4921 gggctgcccg gcatctcgct gcaaccggcg gcgggcgcgc acggcgagat gaccggcgtg
     4981 atgatgatcc gcgccttcct cgacgcgcgc gacggcgtcg gcgagggcga ccagccgcgc
     5041 cgcaacgtga tgctcatccc cgactcggcg cacggcacga accccgcctc ggcgcacctc
     5101 tccggcttca cggtcaagac gattcgctcg acgaacgagg ggctgaccga cctcgggcac
     5161 ctgcgcgagc tatgcgcgtc gggcgacgtg gccggcctga tgctgacgaa cccgaacacg
     5221 gtcggcatct tcgagcgcaa catacaggag atttgccgca tcatccacga cgccgggggc
     5281 ctcgtctaca tggacggcgc gaacatgaac gcgctggtcg gcgtcgcgcg ccccggcgac
     5341 atgggcgtgg acgtgattca cctgaacctg cacaagacct tctccacccc gcacggcggc
     5401 ggcggccccg gctccggccc ctgctgctgc accgccgagc ttgagccgtt cctccccatc
     5461 ccgcgcgtcg tgcgcgaggg cgagcgcttc cggctgactt acgaccgccc gcaatcgatc
     5521 ggtcgcgtca aagccttcta cggcaatttc ggcatgcacc tgcgcgcgct cgcctacacc
     5581 ctgacgcacg ggcgcaccgg cttgcgcgaa gcgaccgagg ccgccgtcct caacgcccgc
     5641 ttcgtcgcgc acggcctcgc cgacacctac gacaagccct tcgactcgcc gcccatgcac
     5701 gaggtcgtct tcaccgacaa gcgccagtcg cgcaagggcg ttcacacgct cgacatcgcc
     5761 aagcggctca tcgactacgg cttccacccg atgacgatct acttcccttt gatcgtccag
     5821 ggcgcgatgc tcatcgagcc gaccgagtcc gtcgggcggc aggagctgca acagttcatc
     5881 gacgccatga agcagatcgc ccgcgaagcg ctcgaagaac ccgacaccgt cttgaaggcg
     5941 ccgcacacca cacgcatcgg tcggctcgac gaagccgccg ccgcccgcaa acccgtcctg
     6001 cgctggcggc ccgccgagaa gaccgccacg gcgtaagtcg tcgcacacca cccacacggt
     6061 taagcgccat gcgacccgat gacggaagtc gcatggcgca ttcgttatcc gccgacgaaa
     6121 tctcaggtat ggtcgagcgg gcttcggaca ccgcgccccc cgcggttgag cacgtgcgtg
     6181 tagaccatcg tcggagtcac gtccttgtgc cccggcagct cctgaatggt gcgtaggtcg
     6241 tgcccgtctt cgagcaggtg tgtggcgaag ctgtggcgga gcgtgtgaca actcgcgtgc
     6301 ttggcgatcc ccgtctcggc cagcgcccct tgacggcctt ctgaatcatc gtctccgaaa
     6361 cgtggtggcg gcggatgtgt ccgtcttcgt gaagacgacc gggagacgct gcggcctctg
     6421 ggcgcgggtc acgccttcga tccggggaag actctgccgc agcacgtcgc ggtaaagaaa
     6481 caggagggcg ctgagggcct gattttgcgt cgaggcggcg acgttcatct cgaccgcgag
     6541 gtgggtcaag aacgcctgaa tctcctcggc gcccatctcc ttcgggtgcc gcttaccatg
     6601 aaaaaggatg aagcgcttga tgaagccggc gtaagcgtct tcggtgcggc ggctgaggtg
     6661 acgcgcccgc gctgtgtgtc tcacttcgtc gaggagctta ggctgtgcca ttccggccct
     6721 ccggcggcgt cctcggccct tccgcccttc cgcctataag gatattatgc ggaacgtcct
     6781 ccgcataata cggcaattcc ggtgttatgc ggaagtcagt tccgcatata cagtagttag
     6841 gtggctacgt gattatgaaa gactcaaagc actttatcct tctgttatgt tgcctcgctg
     6901 tgctagtacc gctttgtgaa ttttcggttg gttctcagca tacttgcggc gggattgaag
     6961 gtagagtaac tactgaagat ggctgggtaa taccaggagc gaatatccgc ctaatgaaca
     7021 aaagaaccaa gcagagtacg agggttcaga ctaacggcga aggagaatat acgatatgc
//
LOCUS       DBNGOH010000187         4949 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2410687, whole genome shotgun sequence.
ACCESSION   DBNGOH010000187 DBNGOH010000000
VERSION     DBNGOH010000187.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4949)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4949)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4949
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2410687"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3853)
                     /locus_tag="AC159P_03550"
     CDS             complement(<1..3853)
                     /locus_tag="AC159P_03550"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase/kinase family
                     protein"
                     /protein_id="HIR3694560.1"
                     /translation="MFGGLRRGRDAREEEPIREELFSVERLEQFAETLAAEHRVTEGP
                     SRGRRLLPRLEENGRALVAAYRTLADAIRAGRLISPAAEWLVDNFHIVEEQLREIRED
                     LPRGYYLELPKLKAGPLAGYPRIYSVALSLIAHTDSRLDTETLRQFVRAYQRVAPLTI
                     GEVWAVAITLRLALVENLRRLATRIVRSRAEREEADTLADKLLEMVERQPESLVPYLA
                     DRLGRKEKIGRTLVVQLAQRLREQDPALAPVFDWLERRLQQQQGAGLEQAVQLEHQRQ
                     AAAQVTVGNIITSMRLLSTLDWRDFFESVSLVDPVLGEDPAGVYWLMDFQTRDRYRHV
                     VERVAKRTHTREAEVASAAVELAAKAHASNPETEAHAHVGYYLIDEGEALLEKTFNYR
                     ARAGERLRRAVLRRPALFYLGTLAALTLFIVAVLVTYGRVAGAPVPLLAALAALSLIP
                     ASELALSVLNWDVTHLFGPRILAKIDTRKGVPEEARTFVVVPTLLTGEGVVDELAETL
                     EVHYLANQDAHVYFALLTDFADADREETPADSALVERALDRLEELNRRHPTESGHARF
                     HLFHRRRVRSEGEGKWMGWERKRGKLHEFNRHLRGARDTTFTVATADAPLLAATRYVI
                     TLDSDTQLPRDAARRLIGVALHPLNRPHFDRARGRVTRGYSILQPRVSVTLVSAARTR
                     FARIFSGHTGIDPYTTAASDVYQDLFGEGIYTGKGLYDVDAFEAALDARVPEYTLLSH
                     DLFESLYARAALVSDLELLDDYPARYDTFAKRQHRWTRGDWQIARWLMPHVPDANRRR
                     VRNTLPLIARWKIADNLRRSLAAPSLVVWLLFAWALLPGSPLVWTLFVVLTLAFPVYA
                     HLTTNVFHHPRGIPWSSHFWSVLGDVRMNTKQTLLTVVFLAHLAYLMCDAVVRTFYRK
                     LVSKKRLLEWMTAAQAERGGARNPSAMLRFMWPAVAVALVSAALVAVFRPAALAVAAP
                     FLLAWALSPLVAHWVSTRLTSERAALDAADAREARLVARRTWRFFEQFVGQADNWLPP
                     DNYQEDPERVAHRTSPTNAGLLLLSTVAARDLGYAATLELVERLELTLGTIDKLQKFR
                     GHLFNWYDTETLAPLAPQYVSTVDSGNLAGHLLALKQSCVELPDRALFGPETLSGLAD
                     TVALLDHEAARLGAVRQRTEAVTIRHLRDEIAACEKLVAGEAPFGVAAWAALFKSLQK
                     HAAEADDIAEALSIEHGSDAFVDVRFWTNALSRQARECARDLQTFAPWGATLAAYVEP
                     IIELSSEENAAAWRETVATLDR"
     gene            4107..4850
                     /locus_tag="AC159P_03555"
     CDS             4107..4850
                     /locus_tag="AC159P_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HIR3694561.1"
                     /translation="MAIAGAAFAAGMINSIAGGGTLVSFPVLLWAGRDAVLANATSTV
                     ALWPASLAGVYGFRREFEGGGWRDVAALAAPSLAGGVLGAVLLLKTPSGVFARLVPFL
                     ILFATGLIMLQEPLSKRVRWAEGERPSRAWWAGATVFQFLVGVYGGYFGAGIGILMLA
                     SLALLGFTDIYRMSAVKNLLAVLINGVAAFYFVVAGAVVWFDVLLMMLAAIAGGYLGA
                     RLAYRLGRRFVRAAVIVIGLVMGLSLLIK"
BASE COUNT          719 a         1492 c         1920 g          818 t
ORIGIN      
        1 cgcggtcgag cgtggcgacg gtttcgcgcc acgccgccgc gttctcctcg gaagacagct
       61 cgatgatcgg ctcgacgtag gcggcgagcg tcgcgcccca cggcgcgaag gtttgcaggt
      121 cgcgggcgca ctcgcgcgcc tgacggctga gcgcgttcgt ccagaagcgc acgtcaacga
      181 aggcatcgct tccgtgctcg atggagagcg cttcggcgat gtcgtccgct tcggcggcgt
      241 gcttctgcaa cgacttgaag agcgcggccc acgcggcgac gccgaaaggc gcttcgcccg
      301 cgacgagttt ttcgcacgcc gcgatctcgt cgcgcaggtg gcggatggtg acggcctccg
      361 tgcgctggcg caccgccccg agccgcgccg cctcgtggtc gaggagagcg acggtgtcgg
      421 cgagtcccga cagggtttcg gggccgaaga gagcacggtc gggcagctcg acgcacgact
      481 gcttcaaggc gagcaggtgg ccggcgaggt tgccgctgtc cacggtcgag acgtactgcg
      541 gcgcgagcgg cgcgagcgtc tcggtgtcgt accagttgaa gaggtggccg cggaacttct
      601 gtagcttgtc gatggtgccg agggtcagct ccagccgctc gaccagttcg agcgtcgcag
      661 cgtagccgag gtcgcgcgcg gcgacggttg atagaagaag cagccccgcg ttggtcggcg
      721 acgtgcggtg cgcgacgcgc tcggggtctt cctgatagtt gtcgggcggg agccagttgt
      781 cggcctgccc gacgaactgc tcgaagaagc gccacgtccg gcgcgcgacg agccgcgcct
      841 cgcgcgcgtc cgccgcgtcg agggccgcgc gctcagaggt caggcgtgtg ctcacccagt
      901 gcgcgaccag cggcgagagc gcccacgcga ggaggaaggg cgcggcgacg gcgagcgccg
      961 cggggcggaa gacggcgacg agcgcggcgc tgacgagcgc gacggcgacc gccggccaca
     1021 tgaagcgcag catcgcggac gggtttctgg cgccgccgcg ctcggcctgc gccgccgtca
     1081 tccattcgag cagccgcttc ttcgagacga gcttgcggta gaaggtgcgc acgacggcgt
     1141 cgcacatgag gtaggcgagg tgcgcgagga agacgaccgt cagcagcgtc tgcttcgtgt
     1201 tcatccgcac gtcgcccagg acggaccaga agtggctcga ccaggggatg ccgcgcgggt
     1261 ggtggaagac gttcgtcgtg aggtgcgcgt agacggggaa ggcgagtgtc aagacgacga
     1321 agagcgtcca cacgaggggg ctgcccggca gcagcgccca cgcgaacagc agccatacga
     1381 cgagcgacgg cgcggcgagg ctgcggcgca ggttgtcggc gatcttccag cgcgcgatga
     1441 gaggtaaagt gttgcgcacg cgtcggcggt tcgcgtccgg cacgtgcggc atgagccagc
     1501 gcgcgatctg ccagtcgccg cgcgtccagc ggtgctggcg cttggcgaac gtgtcgtagc
     1561 gcgcggggta gtcgtccaag agttcgaggt cggagacgag cgcggcgcgc gcgtagagcg
     1621 actcgaagag gtcgtggctc aagagcgtgt attcgggcac gcgcgcgtcg agcgccgcct
     1681 cgaaggcgtc cacgtcgtag agtcccttgc ccgtgtagat gccctcgccg aagaggtctt
     1741 gatacacgtc ggaggcggcg gtcgtataag ggtcgatgcc ggtgtggccg gagaagatgc
     1801 gcgcgaagcg cgtgcgcgcg gcgctcacca gtgtgacgct gacgcggggc tgaaggatcg
     1861 agtagccgcg cgtgacgcgg ccccgcgcgc ggtcgaagtg cgggcggttg agcggatgga
     1921 gcgccacgcc gatcaagcgg cgcgcggcgt cgcgcggcag ttgcgtgtcg gagtcgagcg
     1981 tgatgacgta gcgcgtcgcg gcgaggagcg gcgcgtctgc ggtggcgacc gtgaaggtcg
     2041 tgtcgcgcgc gccgcgcagg tgccggttga actcatggag cttgccgcgc ttgcgctccc
     2101 agcccatcca ctttccctcg ccctcgctgc gcacgcgccg ccggtgaaaa agatgaaagc
     2161 gcgcgtggcc ggactcggtc gggtggcgcc ggttgagttc ttcgaggcgg tcgagcgcgc
     2221 gctcgacgag ggcggagtcc gcgggcgtct cttcgcggtc ggcgtcggcg aagtccgtga
     2281 ggagcgcgaa gtagacgtgc gcgtcctggt tggcgaggta gtggacttcg agcgtctccg
     2341 ccaactcgtc caccacgccc tcgcccgtca ggagcgtggg cacgacgacg aacgtgcgcg
     2401 cctcttcggg gacgcccttc cgcgtgtcga tcttggcgag gatgcgcggg ccgaagaggt
     2461 gcgtcacgtc ccagttgagg acgctcaggg ccagctcgct cgcggggatg agggagagcg
     2521 cggcgagcgc cgcgagcagc ggcacgggcg ctccggcgac gcgaccgtag gtgacgagca
     2581 ccgccacgat gaagagggtg agcgcggcga gcgtgccgag gtagaagagc gcggggcggc
     2641 gcaagacggc gcggcgcagg cgctcgccgg cgcgcgcgcg gtagttgaaa gttttttcga
     2701 gcagcgcctc gccttcgtcg atgaggtagt agccgacgtg cgcgtgcgcc tccgtctcgg
     2761 ggttcgaggc gtgggccttc gcggccagct cgaccgccgc gctcgccacc tcggcctcgc
     2821 gcgtgtgcgt gcgcttggcg acgcgctcga cgacgtggcg gtagcggtcg cgcgtctgga
     2881 agtccatcag ccagtagacg cccgcggggt cttcgccgag gacgggatcg acgaggctga
     2941 ccgactcgaa gaagtcgcgc cagtcgagcg tcgagagcag gcgcatcgag gtgatgatgt
     3001 tgccgaccgt gacctgcgcg gcggcctgcc gctggtgttc gagctgcacg gcctgctcga
     3061 ggcccgcgcc ctgctgctgc tgaagtctgc gttcgagcca atcgaagacg ggcgcgagcg
     3121 ccgggtcttg ctcgcgcagg cgctgcgcga gctggacgac gagcgtgcgc ccgatcttct
     3181 ctttcctgcc gaggcggtcg gcgaggtaag ggacgaggct ttcgggctgg cgctcgacca
     3241 tctccagcag tttatcggcg agcgtgtcgg cctcctcgcg ctcggcgcgg ctgcggacga
     3301 tgcgcgtggc gagccgccgc aggttctcga ccagtgcgag cctgagcgtg atggccacgg
     3361 cccacacctc gccgatggtg agcggcgcga cgcgctggta ggcgcggacg aactggcgca
     3421 gcgtctctgt gtcgagccgg ctgtcggtgt gcgcgatcag cgagagcgcg accgagtaga
     3481 tgcgcgggta gccggcgagc ggcccggctt tgagcttcgg cagctcaagg tagtagccgc
     3541 gcggcaggtc ttcgcggatc tcgcgcaact gctcttcgac gatgtggaag ttatcgacca
     3601 gccactcggc ggcgggcgag atcaagcgcc ccgcgcggat ggcgtcggcc agcgtgcggt
     3661 aggccgcgac gagcgcgcgc ccgttctctt cgaggcgcgg caggagcctg cggccccggc
     3721 tcggcccctc ggtgacgcgg tgctccgccg ccagcgtctc ggcgaactgc tccagccgct
     3781 cgacgctgaa cagctcctcg cggataggct cttcctcgcg ggcgtccctg ccgcgcctta
     3841 gccctccgaa caatcttgtc aggaagcccg gcataaacaa tcagcattaa cgagtcgggg
     3901 caaaaatttt gcgacaagca taccacgtcc tgcgccgccg acgtacggca gcgggcgaac
     3961 tcaatgcgga atgcggaatg cggaacgatg aatggaaggc aggttgcctt ttaccactca
     4021 agccgtttct tattccgcat tccgcattcc gcattcatca ttcctctttt ctctggtagt
     4081 ctgccgggat ggtgtcttgg gaggaggtgg cgattgcggg ggcggcgttc gcggcgggga
     4141 tgatcaactc gattgcgggc ggggggacgc ttgtttcgtt cccggtgttg ttgtgggcgg
     4201 ggagggacgc ggtgctggcg aacgcgacga gcacggtggc tttgtggccg gcttcgttgg
     4261 caggggtgta cgggttccgg cgcgagttcg agggcggcgg gtggagggac gtggcggcgc
     4321 tggcggcgcc gtctttggcg ggcggggttt tgggggcggt gctgctcttg aagacgcctt
     4381 cgggtgtgtt cgcgcggctg gtgccgttcc tgatcctgtt cgcgaccggg ttgatcatgt
     4441 tgcaggagcc gctgtcgaag cgcgtcaggt gggcggaggg cgagcggcct tcgcgtgcgt
     4501 ggtgggcggg ggcgacggtg tttcagtttt tggtgggggt gtacggcggc tacttcgggg
     4561 caggcatcgg gatactgatg ctggcgtcgc tcgcgctgct ggggttcacg gacatctacc
     4621 ggatgagcgc ggtgaagaat ctgctggccg tgttgatcaa cggggtggcg gccttttact
     4681 tcgtcgtggc gggcgcggtg gtgtggttcg acgtgctgct gatgatgctg gcggccatcg
     4741 cgggcggcta cctcggcgcg cggctcgcct acaggctggg ccgccgcttc gtccgcgccg
     4801 cggtcatcgt catcgggctg gtgatggggc tgtcgctgct gattaagtga agtgagcagt
     4861 agcgggcagt aggcagtagg cagcaggcag cgggcagtta aagcagaaga gcgaagatgg
     4921 ttgagaggta aagtccctga catcttcgc
//
LOCUS       DBNGOH010000188         6486 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_394981, whole genome shotgun sequence.
ACCESSION   DBNGOH010000188 DBNGOH010000000
VERSION     DBNGOH010000188.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6486)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6486)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6486
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_394981"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1029)
                     /locus_tag="AC159P_03560"
     CDS             complement(<1..1029)
                     /locus_tag="AC159P_03560"
                     /inference="COORDINATES: protein motif:HMM:NF024816.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3694562.1"
                     /translation="MLARAREIYSKQGARDALPEFEKALALFRVGGDRRHEAITLGLI
                     GNCYKRLADFPKALDYLERALALKRELGDRVEEGKTLSHLGLLFWEQGAYPRAIEHFT
                     QAITIGASLGDRVLEASARNNLALVYDELGEYRRALAEYERALELYRGAQAASGSDPA
                     LGRTIERGISDTIGNIGGTHLLLGDYAEALRYYQQSLAIDERLGLKPAMSQDLGNIAL
                     CYAGLGRTAEALLSLDRALALARAAGMKKEEADWLKAKGSALAQSGRYAEALDHFREA
                     LQTYEQAGLKRELIEALGDAGNLELRLGDAASAEREFRRAAEVARAIGHARGVTLNLL
                     AVGDLEWRR"
     gene            complement(1180..1425)
                     /locus_tag="AC159P_03565"
     CDS             complement(1180..1425)
                     /locus_tag="AC159P_03565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694563.1"
                     /translation="MLKRGVAEFRWQPAADALFYEVFVVTAAGDIVLEQRSEATRLPL
                     PADAPLTPGAKYFVWIRAHLREGKTVNSSVVSFRVAD"
     gene            complement(1894..2043)
                     /locus_tag="AC159P_03570"
                     /pseudo
     CDS             complement(1894..2043)
                     /locus_tag="AC159P_03570"
                     /inference="COORDINATES: protein motif:HMM:NF024881.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
     gene            complement(2025..2921)
                     /locus_tag="AC159P_03575"
     CDS             complement(2025..2921)
                     /locus_tag="AC159P_03575"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR3694564.1"
                     /translation="MAERSINHILRQLQAGETEAAWGQFLEEYSALIYQVVRHFESGG
                     DGASDCFQFVCEQLVKDRFRRLRKFNLEGPATFATWLRAVVRNLCLDRRRKELGRLRL
                     FRSVARLSALDQEVFRCVYERGHSREETALLLAPRYPGLTPDRVLRSVERIEQELTTN
                     QRWRLGVRSRPAARGEATAPDEPHAPLSEAVDPRPDPEDQAFVRERRAALRRALGHLS
                     REDILLLRLRFEEELTLEQVAQLLGLGNAQRADRQIKDVLARLRRDMNFAVGTGADGK
                     IAATSVKQTVQGLAPKKWKKPN"
     gene            3376..4176
                     /gene="kdsB"
                     /locus_tag="AC159P_03580"
     CDS             3376..4176
                     /gene="kdsB"
                     /locus_tag="AC159P_03580"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013279112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HIR3694565.1"
                     /translation="MPPQDSTAVVAVIPARYASSRLPGKPLLDIAGRPMVVRVVERAL
                     EARSVARAVVATDDERVYAAVREAGHECVMTSPDHASGSDRLAEAVAGLEADLIVNVQ
                     GDEPLISPQTIERAVTALMDDEGAAVSTTWEPVETAADVLSGDVVKVVIDERGRALYF
                     SRSPVPFPRDAARRHGSLARALEAEPELLKTFRKHTGLYVYRRAFLLEYTRWPQSALE
                     ITESLEQLRVLERGHSIRVVEAAAPSIGVDTEDDLRRVREMIRQGVGC"
     gene            4222..4605
                     /locus_tag="AC159P_03585"
     CDS             4222..4605
                     /locus_tag="AC159P_03585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694566.1"
                     /translation="MGDWIGLGVIMLVLAGSLWGLSYLGKTREPMSEEEFERRAAEAR
                     GTMSAGVVGLMYALQKLMNPKAAEAVEVQKDLKAGCYNAEYGESGDDLDGDERVGDEP
                     VARRKKDAEPSVEEEDDDEEGKDDA"
     gene            4598..6307
                     /locus_tag="AC159P_03590"
     CDS             4598..6307
                     /locus_tag="AC159P_03590"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013578651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HIR3694567.1"
                     /translation="MPKYIFVTGGVVSSLGKGLAASSIGCLLEARGLSVTLIKLDPYI
                     NIDPGTMSPFQHGEVFVTDDGAETDLDLGHYERFTHARLSQGNNWTTGRIYLSVIEKE
                     RKGDYLGKTIQVIPHITDEIKHAVRAVAEKDAPDVVIVEIGGTVGDIESLPFLEAIRQ
                     MGNEERRGGERGAVFVHLTLVPYIKAAGELKTKPTQHSVRDLREIGIAVDVLLCRSER
                     PLSRDIRSKIALFCNVQEEAVVAAEDVETIYEVPLAFHEQGLDDLIVSELGLRERAAH
                     TDLTRWRELVATIRDPSAGSVTIGIVGKYVELEDSYKSLREALTHGGVANNLRVDVRW
                     VESEGLMDEDYEERLRDFDAILVPGGFGKRGVAGMIRAIQYARKSRTPYFGICLGMQT
                     AVIEYARNVCGLVGADSTEFDAETPYPVIFKLRDLVGVEEMGGTMRLGSWDCRLAPAS
                     LAREVYAGAEETAERHRHRYEFNPEFRAQLERAGLVFSGESRDGRFVEMVELARDAHP
                     WFLGCQFHPEYKSKPLAPHRLFASFVRAAHRNRMRDETAVGGEAEGEAAPHERAQVLA
                     GDD"
BASE COUNT         1075 a         2119 c         2255 g         1037 t
ORIGIN      
        1 gcgccgccat tcgaggtcgc cgaccgcgag caggttgagc gtgactccgc gcgcgtggcc
       61 gatcgcgcgc gcgacttcgg ctgcccgccg aaactccctc tccgccgacg cggcgtcgcc
      121 gagccgcagt tcgaggttcc ccgcatcgcc gagcgcctcg atcagctcgc gtttcagccc
      181 cgcctgctcg taggtctgca aggcctccct gaaatggtcg agcgcctcgg cgtatctgcc
      241 cgactgcgcc agcgcgctcc ctttggcctt cagccagtcg gcctcctcct tcttcatccc
      301 tgccgctcgc gccagcgcga gcgcgcggtc gaggctcaag agcgcttccg ccgtgcgccc
      361 aagacccgca tagcagaggg cgatgttccc caggtcctga ctcatcgcgg gtttcagacc
      421 cagcctttcg tcgatggcca gcgactgctg gtagtagcgc agggcttcgg cgtaatcgcc
      481 gagtagcaga tgcgtgccgc cgatgttgcc gatagtgtcg ctgatgccgc gctcgattgt
      541 tctgccgagg gctgggtcgg agccggacgc ggcctgcgcg ccgcggtaga gttcgagcgc
      601 gcgctcgtac tcggcgagcg cgcggcggta ctcgcccaac tcgtcgtaca ccagcgccag
      661 attgtttcgc gccgaggcct ccagcacgcg gtcgccgagg ctggccccta tcgtgatggc
      721 ctgtgtgaag tgttcgattg cccgggggta tgcgccctgt tcccagaaga gcagtccgag
      781 atggctgagc gttttgccct cctcgacgcg gtcgccgagt tcgcgcttca aagccagcgc
      841 gcgctccaag taatcgaggg ccttcgggaa gtcggccagt ctcttgtaac agttgccgat
      901 caggccgagg gtgatggcct cgtggcgccg gtcgcctccc acgcggaaga gcgcgagcgc
      961 cttttcgaat tcagggaggg cgtcgcgggc gccctgtttc gaataaatct cccgcgcccg
     1021 tgccagcacg gcgtctccgc tctgcgtctc tgtctgtgcg gcggcggggg ttgagggctg
     1081 ggcgtgcggg gttgcgcctc ccggcagcaa cgtgcaggcc gtcaggagag cgcatcgtgt
     1141 gattaaactt cgcatggggg ttgatgatgt gcggggtctt cagtccgcga cgcggaagct
     1201 gacgacactg gaatttaccg tcttgccttc tcgcaggtgt gcgcgaatcc agacaaagta
     1261 tttcgcgccg ggtgtcagcg gggcgtcggc gggcagcggc agccgtgtcg cctcgctccg
     1321 ctgttcgagc acgatgtcgc ccgccgccgt caccacgaag acctcgtaga agagggcgtc
     1381 ggcggcgggc tgccagcgga actctgccac cccgcgtttc agcacctcgc cgtcccgcgg
     1441 agcaagcagt atcggcgaga agccgttcac atcggcgcgg cgcagcgcgg tggcgggcgt
     1501 acgggctgac ctcggcccgc cggcgggtgc tgtcgggcga cgcgccgcgg agccggcggg
     1561 cgtggcggcg cgtgcggtgg cgggtgtcgg ggccccagag tgcggagacg gacccgctac
     1621 gtctacagat tgttgctgag cgatgagcgg ggtctgctcg gccctcgggg cgtccggcgg
     1681 acgcagccgg agggtggcga tgaaaccgac cgtcaggagc agacagacgg ttgccgccgt
     1741 cgccgcccag cgccaacccc ggagaggcgc aggcggctgt gtggacggcg ccagcttcct
     1801 cgctctgaca aggaggtgtg cgggggcctc ggcggacggg ggccagttcg cggagcgggt
     1861 gaggaaagag acctgtgcga gacagcggcg gcagccggcg agatgtgcct cgaccgacgc
     1921 cctgtccgcg ccgccgagcc ggcggtctgc gtaagcggcc agccgcgcct cgccgggaca
     1981 gccccggcgg aagcggcgcc ccttcccgct ccgtaagagg tgtcttagtt cggcttcttc
     2041 cattttttcg gcgcgagacc ctgcacagtc tgtttcacgg acgttgccgc aatttttccg
     2101 tcggcacccg taccgacagc aaaattcatg tcccgtcgga gccgggccag cacgtctttg
     2161 atctgccggt cggcgcgctg ggcgttgccc agcccgagca gttgcgcgac ctgttcgagg
     2221 gtgagttcct cctcgaagcg cagcctgagc agcaggatgt cctctcgcga gaggtggccg
     2281 agcgcgcgcc gcagggcggc cctgcgttcg cgcacaaagg cttggtcttc ggggtctggg
     2341 cgtgggtcta ccgcctccga aagaggggcg tgcggctcgt cgggcgcggt ggcctccccc
     2401 cgtgccgccg gccttgatct gacgccgagc cgccagcgtt gattcgtcgt caactcctgc
     2461 tcgatccgct cgacgctgcg cagcacacga tccggcgtta agccggggta gcgcggggcg
     2521 agcagcagcg cggtctcctc ccgcgagtgc ccgcgttcgt agacgcagcg aaacacctcc
     2581 tgatcgaggg cggagagccg cgcgaccgaa cggaacaggc gcagccgtcc caactccttg
     2641 cggcgccggt cgaggcacag gtttcgcacc acggcgcgca accacgtcgc gaacgtggcg
     2701 ggtccctcaa ggttgaattt acgcaggcga cggaagcggt ctttgacgag ttgttcgcag
     2761 acgaactgga agcagtcgga agcgccgtcg ccgcccgact cgaaatggcg caccacctga
     2821 tagatgagtg ccgaatactc ttcgaggaat tggccccagg ccgcttccgt ctcgcccgcc
     2881 tgaagctgtc tgagaatgtg gttgatcgat ctttccgcca ttgacccgaa tcgcggttca
     2941 gcaggaggtc ggaagtaacg tcggagaacg gcggggacgg gagacggctc tcaccgtcgc
     3001 cgactctacc gaacaacgac acgccgtgcc ggtgtcgccg cgagagtttg cgctggcggt
     3061 agcgtgctgg tggtaggaga aaagggtctt aaggcgctcg cccgccgaaa cggtaggtcg
     3121 gggcctgttg gcttctcgaa aaacgcgtga ttctactccc gcgaggccgg ctttgccaaa
     3181 aaaataatta ccgccgcgcg ccgggcggga caagcaattc acagagccga ggtcgaaggg
     3241 caggccgact cgcgcggcga cagcccgcgg cgggcgggct tctctcttta ttctctccac
     3301 cgtccgcgag gagaaagctt ttcgcttgtc acggcggaag ctcctgggtt attttgtagt
     3361 cccgtgaatc acacggtgcc tccgcaagac tccacggcag tcgtcgccgt catccccgca
     3421 cgctacgctt cgagtcgtct ccccggaaaa cccctgctgg acatcgcggg ccgcccgatg
     3481 gtcgtgcgcg tggtcgagcg cgcgctcgaa gcgcgttcgg tcgcgcgggc ggtcgtggcg
     3541 accgacgacg agagggtcta cgcggcggtg cgcgaggccg ggcacgagtg cgtgatgacc
     3601 agccccgacc acgcttcggg gagcgaccgg ctggccgaag cggtcgcggg gctggaggcg
     3661 gacttgatcg tcaacgtgca gggcgacgag ccgctcatct cgccgcagac aatcgagcgc
     3721 gcggtcacgg ccttgatgga tgatgaaggc gccgccgtct cgacgacgtg ggagccggtc
     3781 gagaccgccg cggacgtttt gagcggcgac gtggtgaagg tcgtcatcga cgaacgcggt
     3841 cgggcgctct acttttcgcg ctcgcccgtg cctttcccgc gcgacgcggc gcgccgccac
     3901 ggctcgctcg cgcgcgcgct cgaagccgag ccggaactct tgaagacttt ccgcaagcac
     3961 acggggcttt atgtctaccg ccgcgccttt ttgttagaat acacgcgctg gccacaaagc
     4021 gcgctcgaaa tcacggaatc gctcgaacaa ctccgcgtgc tggaacgcgg ccactcgatc
     4081 cgcgtcgtcg aggccgccgc gccttccatc ggcgtggaca cggaagacga cctgcggcgc
     4141 gtgcgcgaga tgataaggca gggtgttggg tgttaggtgt tgggtgccag tgaaaaccca
     4201 acacccaaca cccgaattga tatgggcgac tggatcggac tcggcgtcat catgctggtg
     4261 ctggctggct ctctgtgggg gctgtcgtac ttggggaaga cgcgcgagcc gatgagcgag
     4321 gaggagttcg agcggcgcgc ggctgaggcg cgcgggacga tgagcgcggg cgtcgtcggg
     4381 ctgatgtacg cgctccaaaa gctgatgaac ccgaaggccg cggaggccgt cgaggtgcag
     4441 aaggatttga aggccgggtg ttataacgcg gagtacggag agagcggcga cgacttggac
     4501 ggcgacgagc gcgtcggcga cgagcctgtc gcgcggagga agaaggacgc ggagccgagc
     4561 gtcgaagaag aagacgacga cgaagaaggg aaggacgatg cctaaataca ttttcgtcac
     4621 gggcggcgtg gtgtcgagcc tcgggaaggg gctggcggcc tcctccatcg gctgcctgct
     4681 cgaagcgcgg ggcctgagcg tgacgctcat caagctcgac ccctacatca acatcgaccc
     4741 cggcacgatg tcgccgttcc agcacggcga ggtcttcgtc acggacgacg gcgccgagac
     4801 cgacctcgac ctcggccact acgagcgctt cacgcacgcg cgcctctcgc aggggaacaa
     4861 ctggacgacc gggcgcatct acctctccgt catcgagaag gagcgcaagg gcgactacct
     4921 cggcaagacc atccaggtca tcccgcacat cacggacgag atcaagcacg cggtgcgcgc
     4981 ggtcgccgag aaggacgcgc cggacgtggt catcgtcgag atcggcggca cggtgggcga
     5041 catcgagtcg ttgcctttcc tcgaagccat ccgccagatg ggaaacgagg agcggcgagg
     5101 cggcgagcgc ggcgccgtct tcgtccacct cacgctcgtc ccctacatca aggcggcggg
     5161 cgagctgaag acgaagccga cgcagcactc ggtgcgcgac ctgcgcgaga tcggcatcgc
     5221 cgtcgatgtc ctgttgtgcc gcagcgagcg cccgctctcg cgcgacatcc gctcgaagat
     5281 cgcgcttttc tgcaacgttc aggaggaggc cgtcgtcgcc gccgaggacg tggagaccat
     5341 ctacgaagtg ccgctcgcct tccacgagca gggactcgac gacctcatcg tctccgagct
     5401 gggcctgcgc gaacgcgccg cgcacacaga cctcacgcgc tggcgcgagc tggtcgccac
     5461 catccgcgac ccctccgcag gctccgtcac catcggcatc gtcggcaagt acgtcgagct
     5521 tgaggactcg tacaagtccc tgcgcgaggc gctgacgcac ggcggcgtgg cgaacaacct
     5581 gagggtggac gtgcggtggg tcgagtccga aggcttgatg gacgaagact acgaagagcg
     5641 cctgcgcgac ttcgacgcga ttctcgtccc cggcggcttc ggcaagcgcg gagtggccgg
     5701 gatgatccgc gccatccagt acgcgcgcaa gtcccggacg ccctacttcg gcatctgcct
     5761 cgggatgcag acggcggtca tcgagtacgc gcgcaacgtg tgcggcctcg tgggcgccga
     5821 ctcgaccgag tttgacgccg agacgcctta tccggtcatc ttcaagctgc gcgacctcgt
     5881 cggcgtcgag gagatgggcg ggacgatgag gctcggctcc tgggactgcc ggctcgcgcc
     5941 cgcgagcctc gcgcgcgagg tctacgccgg ggccgaggag accgccgagc gccaccgcca
     6001 ccgctacgag ttcaaccccg agttccgcgc acagctcgaa cgcgccggcc tcgtcttcag
     6061 cggcgagtcg cgcgacggtc gcttcgtcga gatggtcgaa ctcgcgcgcg acgcgcaccc
     6121 gtggttcctc ggctgccagt tccaccccga gtacaagtcg aagccgctcg cgccgcaccg
     6181 gctcttcgcc tcgttcgtcc gcgccgcgca ccgcaaccgc atgcgcgacg agacggcggt
     6241 cggcggcgag gccgagggcg aggccgcccc gcacgagcgc gcgcaggtgt tggcgggcga
     6301 cgattgaaac aggctataaa acaaacaggc tataagaaca acaaggagga acaccgtgac
     6361 cgagaagaaa acgcagagcg ccccgttcgg cgaggaagtg gatcagacgg aagtggtcag
     6421 cccgaccgag aagatcgaag gcgctgccga cacgggcgcc gacgtgcccg gcgcgcagaa
     6481 cccgga
//
LOCUS       DBNGOH010000189         4060 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3143703, whole genome shotgun sequence.
ACCESSION   DBNGOH010000189 DBNGOH010000000
VERSION     DBNGOH010000189.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4060)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4060)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4060
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3143703"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            557..1222
                     /locus_tag="AC159P_03595"
     CDS             557..1222
                     /locus_tag="AC159P_03595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694568.1"
                     /translation="MKSLVFASALALSLLGFATPRPAATYNDGPSANGSFQFSADGLA
                     KRVEFEARTHKDGTTTGRMTFSGPAEVPDQDVDGEGGPATGAQLVDIFTKVEFDCLVV
                     EGNRAVMSGVVTESTVGEYIGRRALLAVEDNGEGVGAAAPDRLTWGVYRSTERGWVPV
                     DAERPDDNGALLEWVATDAEREDDAGVPSRRSEVVGCRSFPLSSYSFVDVKHGDGNIQ
                     VRP"
     gene            2010..2285
                     /locus_tag="AC159P_03600"
     CDS             2010..2285
                     /locus_tag="AC159P_03600"
                     /inference="COORDINATES: protein motif:HMM:NF024139.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR3694569.1"
                     /translation="MAKKNLEGSEMLTVQEAAERKGVTDVRVYQWLAEGRLTKHEKYG
                     RTLVDAAELDALQELRRGPKPKAENAPSSGGLVVVKPKRAAKRGGKK"
     gene            2404..3021
                     /locus_tag="AC159P_03605"
     CDS             2404..3021
                     /locus_tag="AC159P_03605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694570.1"
                     /translation="MKHITNFVPQITAAALLAAAAATDAAAQQPPPNPADREAINREL
                     RERSRDLDTLEKTRLPPPERTGARLDLARVEEDHLRLQQAANGLHQAVAQGGALDLKA
                     VAKSASEIRERAVRLGSALALPKTEKEEKRPKPAAIADDEGLRESLAALHELIYRFAR
                     SPVFTEASLVDAEHSAKARRDLDEIIALSGRVKKGCQELEKGGKQ"
     gene            3018..3179
                     /locus_tag="AC159P_03610"
     CDS             3018..3179
                     /locus_tag="AC159P_03610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694571.1"
                     /translation="MTYVLLCPGFDPVTLVFILAVLAGAQVAAGAVVGLAVARVIRAV
                     KRSRPVRLR"
     gene            3260..3487
                     /locus_tag="AC159P_03615"
     CDS             3260..3487
                     /locus_tag="AC159P_03615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694572.1"
                     /translation="MRFIKIGDSYAVNADRIMDATYTARGYDETGEGAGEHTQAAVTI
                     RLAGATEVEERTLEGQAAESFWSNLTGTALR"
     gene            3524..3685
                     /locus_tag="AC159P_03620"
     CDS             3524..3685
                     /locus_tag="AC159P_03620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694573.1"
                     /translation="MPKWLLFGLAAVGQFAVAAAVYLNSDRVVIPGVLALAGLLMLFA
                     AVGSAMGKE"
BASE COUNT          710 a         1242 c         1409 g          699 t
ORIGIN      
        1 atccccttca agacaagtga cagacacgtc agtgcgtaca gcagtacgac gaacagcaag
       61 gcgacggagg tctcgtacgt catctccccg gcattctagg cgagagggtg acaacccgca
      121 tcggacaaag ggacagttcc gcatagtagg aatgtcacgg gcgcgcgtgg aagccgcgcc
      181 ggattcgtcc tttccatcaa gacagcggga gggcggtcgg cgggcaccgc gggcaagggc
      241 gagtaagggg agagactttc caagcccgcc gcgcccgtgc ctgccgccct ccccttccct
      301 actccttcgc aagcgacgag ggtgcggagc ctctccccac gcctttccct ttcaggcgcg
      361 aacccgtgcg cgtgcccgtt gactttcggc gccgttacct atattaagtc ccgaacactc
      421 acctcatgaa ttcctccggc agtgttttga gtcggcactg tcgcggcctc cgaagccgcg
      481 tagggtattt ccgagatgcg tgcccgtaag aatccgccct gtcacaacac ggcgttcaaa
      541 tcaaggagtt ccccagatga agagtctcgt attcgcctcg gcgctggcgc tgtccctgct
      601 cggcttcgcg accccgcgcc ccgccgccac atacaacgac gggccgtccg ccaacggcag
      661 tttccaattc tccgcggacg gcctcgccaa gcgcgtcgag ttcgaggccc ggacgcacaa
      721 ggacggcacg accaccgggc ggatgacttt cagcggcccc gccgaggtgc ccgaccagga
      781 cgtggacggc gaaggcggcc cggccaccgg cgctcagctc gttgacatct tcacgaaggt
      841 ggagttcgac tgcctggtcg tcgaggggaa ccgggctgtg atgagcggcg tcgtcacgga
      901 gtccacggtc ggcgagtaca tcgggcgacg ggcgctgctg gcggttgagg acaacgggga
      961 gggcgtgggc gccgcggccc cggacaggct gacgtggggc gtctacaggt ctacggagcg
     1021 cgggtgggtg ccggtggacg ccgagcgccc ggacgacaac ggggcgctgc tggagtgggt
     1081 ggcgacggac gcggagcgcg aggacgacgc gggggtgccg tcgcggagga gtgaggtggt
     1141 aggatgccgg agcttcccgc tctcgtccta ctcgttcgtc gatgtgaagc acggcgacgg
     1201 caacatccag gtccggccct gacgccgacg aaaattccgc acacgaaaag ggcgcggagc
     1261 ctcaatccgc gccccttccc ttttcagcga atgaccgggc aggccgcgaa cctctcctcc
     1321 acctgaaagg cgacggtctc gcgctcggtg tagtcggtca gggtgcgtct cgcgggcttc
     1381 ctcggcgagc agttcgccga cgcggcggac gttgcgtggg tcgctcaact tggggccgcg
     1441 cgttgcggcc ctgcgtttcg tgttatcctt catgtgcttt tcccttcatt ggtctgaggg
     1501 tggaaagtag ggggctaggt gagcgcgagc acgctttcct agcccttccc ttttagtcgt
     1561 cgccgtcgta ccgctcgccg ttggctaagg cttcgggcgc gcggcgcatg tcgcggatcg
     1621 cccgcaggta gatgacgagc gccgtcgtgt gcttgcagac gagcggcagg cggtcgcctt
     1681 tgctcggcga gccggcttcg cagtagcagg tgacgaaggt cttaccgttg aggcgcgtga
     1741 cgcggacggg gtagctgtgg gctttggctt tgttggcgac gatgtacagg ccgggttcgt
     1801 ggcgacggat tgaatgcggt tccttgcgaa gtctggcggc ggctttggcg aactgcgcgg
     1861 ggtgttgaat ccgaattatc tctgttactc cttccgattt cgcttgcgtc aggtagccct
     1921 ttgaattaaa attccttagc ccgaactaag ggttacgatc tatatttcct taattggggg
     1981 caaggtaatt ctttcttatc gtggctaata tggctaagaa aaaccttgag ggttccgaga
     2041 tgctgactgt gcaggaggca gcggagagga agggcgttac agacgttcgg gtttaccagt
     2101 ggctcgcgga aggccgtctg accaaacatg agaagtacgg gcgcacattg gtcgatgcag
     2161 cagaattgga cgctctccaa gagttgcggc gcgggccgaa gccgaaggcc gaaaatgccc
     2221 ccagttctgg cgggctggtg gtggtcaagc cgaagcgcgc ggcgaagaga ggcgggaaga
     2281 agtgagcagc acggcgtggg gattcttgtc ggggtactct tcgccctgct ggtgctcgcg
     2341 gcgcgcgcct acctgaagag aggcgggaag tggtgagcgg tcattgagat aaggaggccg
     2401 aggatgaagc atataacgaa ttttgtgccg cagatcaccg cggcagccct cctcgcggct
     2461 gccgccgcca ccgacgccgc ggcccagcag ccgccgccaa accccgctga ccgcgaggcg
     2521 atcaaccgcg agctgcgcga gcgcagccgc gacctcgata cgctggagaa gacgcggctc
     2581 ccgccgcctg agcgcacggg agcgcggctc gacctggcgc gggtcgagga agaccacctg
     2641 cgtcttcagc aggcggccaa cggcctgcac caggcggtcg cgcagggcgg cgcgctcgac
     2701 ctcaaggcgg tggccaagtc ggcctccgag atcagggagc gcgccgtgcg gctcggctcc
     2761 gccctggcgc tgcccaagac agagaaagag gagaagcgcc cgaagcccgc ggccatcgcg
     2821 gacgacgagg ggctgaggga gtcgctggcc gcgctgcacg agttgatcta ccggttcgcg
     2881 cgcagccccg tcttcaccga ggccagcctc gtggacgccg agcattcggc gaaggcgcgg
     2941 cgcgacctag acgagatcat cgcgctgagc gggcgggtca agaagggctg ccaggagttg
     3001 gagaagggcg ggaagcaatg acctacgtac tcttatgccc cggcttcgac ccggtgacgc
     3061 tggtgttcat cttggccgtg ctggcgggcg cccaggtcgc ggcgggcgcc gtcgtggggc
     3121 tggccgtcgc tcgcgtcatc agggccgtga agagatcaag gccggtgcgg ctcaggtagc
     3181 cgagaagtca gccggtcact ccttaccgcc tggggcttac gtgcggtggc tctgaccgcg
     3241 aagggggaat tgattaacca tgcgcttcat caagatcggc gactcttacg ccgtcaacgc
     3301 cgaccgcatc atggacgcga cctacacggc cagaggctac gatgagaccg gcgaaggggc
     3361 cggcgagcac acccaggctg ccgtcaccat caggctcgcc ggggcgaccg aggtggagga
     3421 gcgcacgctc gaagggcagg ccgcggagag cttctggagc aatctgacgg gaaccgccct
     3481 gcgttagggc cgaacgcggg agaccctttg cgaaggagca acgatgccta agtggctgct
     3541 gttcggcctc gccgccgtag gccaattcgc ggtggccgcc gccgtgtact tgaacagcga
     3601 ccgggtggtg atccccggcg tcctggcgct cgcgggcctg ctcatgctgt tcgccgctgt
     3661 ggggagcgcg atggggaagg agtgacggcg tgaagggcgt atgaatcatt cggaaggatg
     3721 tatgattgtg tgcggatgtg tgtgcggcgg cttcagcctt gatctcctgc cccgacaacc
     3781 tcaaacgtct taacacttac gaaagcccgt gataaacctg cacttccgct tcaacgtctg
     3841 aagcccgccc cgagccgcga agtcttcaca caccatcgcg cgcgaactgg ccgcccgcgc
     3901 gtctgtacgc gcggcggagc gcctgaaccc ggcgcgaccc cgcgtgggct caagtgaaaa
     3961 ggggcacggc gcagtccggt cggactgcgc cgtgcccctc cgaggttgtt tgtcaccgcg
     4021 gcgcgggcgg aaaatcgttg acgcgatttt ccgcccgcgc
//
LOCUS       DBNGOH010000190         5671 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_761226, whole genome shotgun sequence.
ACCESSION   DBNGOH010000190 DBNGOH010000000
VERSION     DBNGOH010000190.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5671)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5671)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5671
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_761226"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(106..1203)
                     /locus_tag="AC159P_03625"
     CDS             complement(106..1203)
                     /locus_tag="AC159P_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016951652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HIR3694574.1"
                     /translation="MSSVLVALIEEPRAPVEVREVAEPELETDSALLEVELSEVCGTD
                     VHLHEGRLAGVPYPLVPGHVSVGRLGKIRGRVLDVEGRPFKEGDRVTFLDVHRTCGAC
                     WFCLVGKASTRCPHRKVYGITYGLADGLCGGWAEKIYLKPGTRVIRLEGVDAERFMAG
                     GCSLPTALHAVERAGVKIGDTVLVLGSGPVGLSAVAFSLMSGALKVLCVGAPAQRLEA
                     ARAVGAAATLDIETHDEETRARWVSDEMGGRGADVTIEATGVPAAVVQAMRFTRDAGR
                     VVVVGQYTDHGETAFNPHQDLNRKHLDVRGCWGSDFSHFYRGALVMGDAARSAAWASL
                     SLERYGLARAGEALAAVAAGRVVKALIDPRV"
     gene            complement(1298..2221)
                     /locus_tag="AC159P_03630"
     CDS             complement(1298..2221)
                     /locus_tag="AC159P_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003493856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HIR3694575.1"
                     /translation="MRKLHYHRVDVFTDRAFGGNPLAVFTNGRGLTTETMQAIAKEFN
                     LSETTFVLTPDDARHDYRVRIFTPANELPMAGHPTVGTSFVLAREHMVEGADGAPVRV
                     TLEEGVGPIPVRIEFAGGQPVYAEMTQPLPVFGPRLEDLAPVAEMLSLDAADIDEQLP
                     DEVVSCGVPFLYVPLKTLDAARRARPRADLMERALAGVVPPEVLVFTREVEHAGSTVH
                     SRMFAPPLGITEDPATGAASGPLGAYLVRHGLVPFDGGAAEIVSEQGIEMGRPSFIKI
                     RIEAEGEEITGVHVGGQCVYMGEGFIEVNAE"
     gene            complement(2345..3067)
                     /locus_tag="AC159P_03635"
     CDS             complement(2345..3067)
                     /locus_tag="AC159P_03635"
                     /inference="COORDINATES: protein motif:HMM:NF014035.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HIR3694576.1"
                     /translation="MSFTTTLLLALFILFVSTLVRSALGFGDALVAMPLLALLVGVKT
                     ATPVVAFAASTIALLILLKSWRDVDVRATWRLVVSSLVGIPFGLLLLKSAPEVYVKAA
                     LGALLIVYGLYNLVAPRLPVVESEGLAYVFGFAAGVLGGAYNTNGPPVVVYGALRRWP
                     PENFRATLQGCFVLTGLSILVGHGLAGLWTPLVLQLYLASLPAILLAVFLGARLNRRI
                     PRESFTRVVYAFLVVVGVLFLL"
     gene            complement(3180..4364)
                     /locus_tag="AC159P_03640"
     CDS             complement(3180..4364)
                     /locus_tag="AC159P_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003353106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trans-sulfuration enzyme family protein"
                     /protein_id="HIR3694577.1"
                     /translation="MKREDFEEAGTQTTVVHAGRALNATRAVSPPIWQTTTFTADTPR
                     DFAEEAKAVRPAHFYTRYGNPTHKEVEEVVATLEGGEAALLTSSGMGAIFNVVMSLVG
                     SGDHVVSQRSLYAGTEELMKSVLPRFGVEVTYVDQTRPEEFAAALRPNTRLVYAETPT
                     NPLMRLTDLAALAELARPRGVTTVVDNTFATPVNQRPLAFGIDVVVHSATKYLGGHHD
                     VTAGCIVSTSEFVERAWRFSLVAGACLGPFDGWLLLRGLRTLGLRVERHNRNALALAR
                     FLERHPHVERVHHPGLASHPQHELARRQMRGFTGMLGVELRGGFAEAKRFTKALRLAT
                     YAASLGGFETLVVHPAAMWGLQLTPVQRAEMGVSDGLVRVSVGLEDERDLIADFAQAL
                     DA"
     gene            4520..4705
                     /locus_tag="AC159P_03645"
     CDS             4520..4705
                     /locus_tag="AC159P_03645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694578.1"
                     /translation="MNARDVFPSRFHRNPDEGPDTNMDGFDFRLAKLDEHGGDYNRAE
                     MVRAFINSIEYRERFQW"
     gene            complement(4875..>5671)
                     /locus_tag="AC159P_03650"
     CDS             complement(4875..>5671)
                     /locus_tag="AC159P_03650"
                     /inference="COORDINATES: protein motif:HMM:NF024874.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3694579.1"
                     /translation="RPAEWGDERLTVAWLGHATVLMNFYGTWLLTDPALRPRVGVSAG
                     VVTLGPRRLVRPALGVKELPALDAVLVSHAHMDHCDLGTLRRLPRASAAIVPKGMRDL
                     VRRFARVEELQWGEAVEVGGARVELIEVSHWGARRLTDRHRGYGGFLIEKNGRAVVFG
                     GDTAYTPAFSRLLAERGKRIDLAVLPVGGYNPYLQVHANPEQAWAMSREMGARFILPM
                     HHSTFKLSREPASEPVSRLMEAAGRDRWRVALTEVGQTWMLDDSRQ"
BASE COUNT          933 a         1996 c         1886 g          856 t
ORIGIN      
        1 gcagaggccg cagagaagaa gttgatggct ttctctgcgg cctctgcgcc tctctgtgtc
       61 ctctgcgtcg aaatgtgttc gcggtaaact caagggccaa ccgctctaaa cgcgcgggtc
      121 gatgagcgcc ttcacgacgc gcccggcggc gactgccgcg agcgcttcgc ccgcgcgtgc
      181 gaggccgtag cgttcgagcg agagcgaggc ccacgccgcg gagcgcgcgg cgtcgcccat
      241 cacgagcgcg ccgcggtaga agtgcgagaa gtccgaaccc cagcagccgc gcacgtcgag
      301 gtgtttccgg ttgaggtcct ggtgcgggtt gaaggccgtc tcgccgtgat ccgtgtactg
      361 cccgaccacc accacgcgcc ctgcgtcgcg cgtgaagcgc atcgcctgca cgaccgccgc
      421 cggcacgccc gtcgcctcta tcgtcacgtc cgcgccgcgc ccgcccatct catccgacac
      481 ccaacgggcg cgcgtctcct cgtcgtgcgt ctcgatgtcg agcgtcgccg ccgcgcccac
      541 cgcccgcgcc gcttcgaggc gctgcgccgg agcgccgacg cacagcacct tcagcgcgcc
      601 gctcatcagc gagaacgcga ccgcggagag gcccaccggc ccgctcccca acacgagcac
      661 cgtgtcgccg atcttcaccc ctgcgcgctc gaccgcgtgc agcgccgtcg gcagagagca
      721 cccgcccgcc atgaagcgct cggcgtccac gccttcgagc cgtatcacgc gcgtccccgg
      781 cttcaggtaa atcttctcgg cccacccgcc gcagaggccg tccgcgaggc cgtacgtgat
      841 gccgtagacc ttgcggtgcg ggcagcgcgt cgaagccttc ccgacgaggc agaaccagca
      901 cgcgccgcac gtccggtgca cgtcgaggaa cgtcacgcgg tcgccctcct tgaacggcct
      961 gccctcgaca tcgagcacgc gcccgcgaat cttccccagc cgccccaccg agacgtggcc
     1021 cggcacgagc gggtacggca cgcccgccag cctgccttcg tgcaggtgca cgtccgtgcc
     1081 gcacacctcg ctcagctcga cctccagcag cgccgagtcc gtctccagct caggctccgc
     1141 gacctcgcgc acctcgaccg gcgcgcgcgg ctcctcgatc aacgccacca gcacactcga
     1201 catctctcaa cctcttaaag taacgatgaa tgcagagtgc agaatgatga ataaagaact
     1261 gtttgtctgt tatccatcat tctgcactct gcattcatca ttctgcattc acctcgatga
     1321 agccctcgcc catgtagacg cactgcccgc cgacgtggac gccggtgatc tcttcgccct
     1381 ccgcttcgat cctgatcttg atgaagctcg ggcggcccat ctcgatcccc tgctcgctga
     1441 cgatctccgc cgcgccgccg tcgaacggga cgaggccgtg gcgcacgagg taggcgccga
     1501 gcggcccgga ggcggcgccc gtcgccgggt cttcggtgat gccgagcggc ggggcgaaca
     1561 tgcggctgtg gacggtggag ccggcgtgct cgacctcgcg cgtgaagacc agcacctcgg
     1621 gcggcacgac gcccgcgagc gcgcgctcca tcaggtccgc gcgcgggcgc gcgcggcgcg
     1681 cggcgtcgag cgtcttcagc ggcacgtaga ggaacggcac gccgcacgag acgacttcgt
     1741 cgggcagctg ctcgtcgatg tccgccgcgt cgagcgagag catctcggcg acgggcgcga
     1801 ggtcttcgag gcgcgggccg aaaacgggga gcggctgcgt catctcggcg tagacgggtt
     1861 ggccgcccgc gaactcgatg cggacgggga tggggccgac gccctcttcg agcgtgacgc
     1921 ggacgggcgc gccgtccgcg ccctcgacca tgtgctcgcg cgcgagcacg aacgacgtgc
     1981 cgacggtcgg gtgcccggcc atcggcagct cgttggcggg cgtgaagatg cggacgcggt
     2041 agtcgtgccg cgcgtcgtcc ggcgtcagca cgaaggtcgt ctccgacagg ttgaactctt
     2101 tggcgatggc ctgcatcgtc tccgtcgtca gccctcgccc gttcgtgaac acggccagcg
     2161 ggttcccccc gaacgcccgg tcggtgaaca cgtcaacgcg gtggtaatgt aattttctca
     2221 tctctttgaa gctgttagct gttagctatt agcggttagc atttaatttt ctgcgagtca
     2281 caatcttcgt cgcaccatgc ggcctgtgaa gcacatgata gctgaaagct aagagctaag
     2341 agcttcacag caagaacagc acgccgacga cgacgaggaa ggcgtagacg acgcgcgtga
     2401 aagactcgcg cgggatgcgg cggttgagcc gcgcgccgag aaagacggcg agcaggatcg
     2461 ccggcagcga ggcgaggtag agttgcagga cgagcggcgt ccacaagccc gccagcccgt
     2521 ggccgacgag gatcgagagg ccggtcagca cgaagcagcc ttgcagcgtc gcgcggaagt
     2581 tctcgggcgg ccagcgccgc agcgcgccgt agacgacgac cggcggcccg ttcgtgttgt
     2641 acgcgccgcc aagcacgccc gccgcgaagc cgaagacgta cgccagccct tcgctctcga
     2701 ctacggggag ccgcggcgcg acgaggttgt acagcccgta gacgatgagc agcgcgccga
     2761 gcgccgcctt cacgtaaacc tcgggcgcgg acttcaagag cagcagcccg aacggtatcc
     2821 cgacgagcga cgagacgacg agccgccacg tcgcgcgcac gtccacgtcg cgccagctct
     2881 tcaagagaat caggagcgcg atggtcgagg ccgcgaacgc cacgaccggc gtcgccgtct
     2941 tcacgcccac caacaacgcc agcagcggca tcgccaccag cgcgtcgccg aagcccagcg
     3001 ccgagcgcac gagcgtcgag acgaagagga tgaagagcgc gagcagcagc gtcgtcgtga
     3061 aactcatacg gattgcggat tgcggattgc ggattgcgga ttagcgtgct gctgtaactc
     3121 gaagcatgat ttcgatagtt cgattgtcga tgtcgaacaa tccgcaatcc gcaatcgcct
     3181 taagcgtcga gcgcctgcgc gaagtccgcg atcaagtccc gctcgtcttc gaggccgacg
     3241 gagacgcgga cgaggccgtc cgagactccc atctccgcgc gctgcacggg cgttagttga
     3301 aggccccaca tcgccgcggg gtggacgacg agcgtctcga agccgccgag gctggcggcg
     3361 taggtcgcga gccggagcgc cttcgtgaag cgcttggcct ccgcgaagcc gccgcgcagc
     3421 tcgacgccga gcatgccggt gaagccgcgc atctgccgcc gcgccagctc gtgctgcggg
     3481 tgcgatgcga ggccggggtg gtgcacacgc tccacgtgcg ggtggcgttc gaggaaccgt
     3541 gcgagcgcga gcgcgttgcg gttgtggcgc tcgacgcgca ggcccaacgt gcgcagaccg
     3601 cgcagcagca gccagccgtc gaaggggccg aggcacgcgc ccgcgacgag cgagaagcgc
     3661 cacgcgcgct cgacgaattc gctcgtcgag acgatgcacc cggcggtcac gtcgtggtgg
     3721 ccgccgagat acttggtcgc gctgtggacg accacgtcga tgccgaaggc gagcgggcgc
     3781 tggttgacgg gcgtggcgaa ggtgttgtcc acgacggtcg tgacgccgcg cgggcgcgcc
     3841 agctccgcga gggcggcgag gtctgtcagg cgcatgagcg ggttggtcgg cgtctcggcg
     3901 tagacgagcc gcgtgttggg gcggagcgcg gcggcgaact cctcgggccg cgtctggtca
     3961 acgtacgtga cctcgacgcc gaagcgcggc agcacactct tcatcaactc ttcggtgccc
     4021 gcgtacagag agcgctgcga gacgacgtgg tcgccgctgc cgacgagcga catcaccacg
     4081 ttgaagatcg cgcccatgcc ggaggaggtc aggagcgccg cctccccgcc ttcgagcgtg
     4141 gcgacgacct cctcgacctc cttgtgcgtc gggttgccgt agcgcgtgta gaagtgcgcg
     4201 ggccggacgg ccttcgcctc ctccgcgaag tcgcgcggcg tgtcggcggt gaaggtcgtc
     4261 gtctgccaga tcggcgggct gacggcgcgc gtcgcgttga gcgcgcggcc cgcgtgcacc
     4321 acggtcgtct gcgtccccgc ctcctcgaaa tcttctctct tcatgcgaac ctcccgccgc
     4381 cgcgcgcgtc gcgtcccgtg cgtgtctccg cgcgcctcgc gcgcggccat cttacaacgt
     4441 aaagctcttc cgcgtggatt gccggtgcca ttttacgccg ggccgccggg ccaccggctg
     4501 caacggtcgc gcgaaaaaca tgaacgcgcg cgacgtattt ccctcccgct tccaccgcaa
     4561 cccggacgaa gggccggaca ccaacatgga cggcttcgac ttccggctcg ccaagctcga
     4621 cgagcacggc ggcgactaca accgcgccga gatggtgcgc gccttcatca actcgatcga
     4681 gtaccgcgag cgcttccaat ggtaaacagt aggcggtagg cagtaggcgg taggcagtta
     4741 aagtcaggag gcttgagagc taacgtctca agcctcctgc tttaccgctt gactcaagga
     4801 agacgcttgg gactttacct ctcaagcatc atcgcccttc ttgctttgac tgcctaccgc
     4861 ctactgccta ctgcctactg cctactgtcg tcgagcatcc acgtctggcc gacctccgtc
     4921 aacgcgacgc gccagcggtc gcggcccgcg gcttccatca gacggctgac gggttcgctc
     4981 gccggctcgc ggctgagctt gaaggtcgag tggtgcatag ggaggatgaa gcgggcgccc
     5041 atctcgcgcg acatggccca cgcctgttcg gggttggcgt ggacttgcag gtaagggttg
     5101 tagccgccga cgggcagcac cgcgaggtcg atgcgcttgc cgcgctcggc gagcagccgc
     5161 gagaaggcgg gcgtgtaggc cgtgtcgccg ccgaaaacga cggcccgtcc gttcttctcg
     5221 atcagaaagc cgccgtagcc gcggtgcctg tccgtgaggc gccgcgcgcc ccagtggctc
     5281 acctcgatca gctcgacgcg cgcgccgccg acctcgacgg cctcgcccca ctgcaactcc
     5341 tcgacgcgcg cgaagcggcg caccagatcg cgcatcccct tcggcacgat ggccgccgac
     5401 gcgcgcggca gccgccggag cgtccccaga tcgcagtgat ccatgtgggc gtgcgagacg
     5461 agcacggcgt cgagcgcggg cagctccttc acgccgagcg cgggccgcac cagccggcgc
     5521 ggccccagcg tcacgacccc cgcgctgacg cccacgcgcg gcctcaaggc cgggtcggtc
     5581 agcagccacg tcccgtaaaa attcatcagc acggtggcgt gtccgagcca cgccaccgtc
     5641 aaacgctcgt cgccccactc cgcagggcgc g
//
LOCUS       DBNGOH010000191         1543 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3878383, whole genome shotgun sequence.
ACCESSION   DBNGOH010000191 DBNGOH010000000
VERSION     DBNGOH010000191.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1543)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1543)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1543
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3878383"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            74..814
                     /locus_tag="AC159P_03655"
     CDS             74..814
                     /locus_tag="AC159P_03655"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3694580.1"
                     /translation="MSATDILNEVVAGGRTPRPLGEGIYSVLAGEAGGHGYDRRAAVY
                     DAVVGTRVYNRVMWGAARGAYEAFARRALASGGDGLVLDAPCGSLLFSAAAYAESGRR
                     IVALDQSLGMLRRARARLAESAGAVPENVVFLQADLDDLPFVAGSFDTVLSMNVLHLY
                     RGDASALVSNLGGLLKDGGRLSLTSLVRGGRLVGDGYLRLLHRAGEFVRPRSEAELRG
                     LLEKSLGQKVDCETKGNMAYATAVVRRA"
     gene            836..>1543
                     /locus_tag="AC159P_03660"
     CDS             836..>1543
                     /locus_tag="AC159P_03660"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR3694581.1"
                     /translation="MNPIAFIRRRGAFDASRGRERALPYVLAAPTIAVLFALSIYPLV
                     YAVKVSLQTGAGARERWTLENFARLASDEFFLDALGHTLIYAAVALTCEFLLGLALAV
                     LLNREMRARGLLRAALLVPLMLPPVVAGVVWRLMLNPNFGAVNGTLRGLGVNTEALTW
                     TASPKLALLSVIAVDVWQWTPFMFLVLLAGLQAIPQEPYEAARVDGASGWQTFRHVTL
                     PLLKPAILIALLLRTMDL"
BASE COUNT          203 a          461 c          604 g          275 t
ORIGIN      
        1 cgcgggagat gagcgctccg tcttcgtcgg ttgacgacgg gcgtcgtcaa cgagcggcgg
       61 cgccgtttga cagatgagcg ccacggacat actgaacgaa gtcgtcgccg gagggcggac
      121 gccgaggccg ttgggcgagg gcatctattc ggtgctggcg ggcgaggcgg gcgggcacgg
      181 ttacgaccgc cgggcggcgg tctatgacgc cgtcgtgggg acgcgggttt ataaccgcgt
      241 gatgtggggc gcggcgcggg gcgcatacga agcgttcgcg cggcgcgcgc tggcgtccgg
      301 cggggacggt ttggtgctcg acgcgccttg cgggtcgctt ctgttcagcg cggcggcgta
      361 cgccgaaagc gggcggcgca tcgtcgctct cgaccagtcg ctcggcatgt tgagacgtgc
      421 gcgggcgcgg ctcgcggagt cggcgggcgc ggtgcccgag aacgtggtgt tcctgcaagc
      481 ggacctcgac gacctgccgt tcgtcgcggg gagtttcgac acggtgctct cgatgaacgt
      541 gctgcacctg tatcgcggcg acgcgtcggc gctggtgtcg aatctcgggg gactgttgaa
      601 ggacggcggg cgtttgtcct tgacgagtct cgtgcgcggc ggtcgcttgg tcggcgacgg
      661 ctacctgcgt ctgctgcacc gcgcgggcga gttcgtccgc ccgcgcagcg aagccgagct
      721 gcgcggcctg ttggaaaaat cgctcggcca aaaagtcgat tgcgagacga aggggaacat
      781 ggcctacgcg accgccgtcg tacgacgagc atgaggtcgg tcgcctcgaa agagtatgaa
      841 cccgattgcc ttcatcagac gacgcggcgc gttcgatgcg tcgcgcgggc gcgagcgggc
      901 gctgccgtac gtgctggcgg cgccgaccat cgccgtgctg ttcgcgctgt cgatttatcc
      961 gctcgtctac gcggtgaaag tcagtctgca aaccggcgcg ggcgcgcgcg agcgttggac
     1021 gctggagaac ttcgcgcggc tcgcgtcgga cgagttcttc ctcgacgcgc tcgggcacac
     1081 attgatttac gcggcggtcg cgttgacgtg cgagttcttg ctggggctgg cgctcgcggt
     1141 gctgctcaat cgggagatgc gcgcgcgggg gctgctgcgg gcggcgctgc tggtgccgct
     1201 gatgctgccg ccggtggtgg cgggcgtcgt gtggcggctg atgctgaacc cgaacttcgg
     1261 cgcggtcaac gggacgctgc gggggctagg cgtgaacacg gaggcgctga cgtggacggc
     1321 gtcgccgaag ctggcgctgc tgtcggtcat cgcggttgac gtgtggcagt ggacgccgtt
     1381 catgttcctc gtgctcttag cggggctaca ggccatcccg caggagcctt acgaggcggc
     1441 gcgcgtggac ggcgcgagcg ggtggcagac cttccgccac gtcacgctgc cgctcctgaa
     1501 gcccgcgatc ctgatcgcgc tgctcttgcg gacgatggac ctg
//
LOCUS       DBNGOH010000192         1675 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3511120, whole genome shotgun sequence.
ACCESSION   DBNGOH010000192 DBNGOH010000000
VERSION     DBNGOH010000192.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1675)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1675)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1675
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3511120"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..596
                     /locus_tag="AC159P_03665"
     CDS             <1..596
                     /locus_tag="AC159P_03665"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF017024.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0016726 - oxidoreductase activity, acting
                     on CH or CH2 groups, NAD or NADP as acceptor [Evidence
                     IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HIR3694582.1"
                     /translation="AEELAEILGGHDAAIDFTIAEAVPRHAEACALARVPLVEGTTGW
                     HAQLEDVRQLFERENGALIYGANFSVGVNLFYRVAARAAELFAAVEGYDPFIEEAHHS
                     RKRDAPSGTALRLREIVAEHAGREVPIASTRAGHIPGTHRVGFDSAADTVTLTHTARS
                     REGFAAGAVLAARWIGGRRGVYEFAEMFDEILKGRES"
     gene            710..1636
                     /gene="dapA"
                     /locus_tag="AC159P_03670"
     CDS             710..1636
                     /gene="dapA"
                     /locus_tag="AC159P_03670"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HIR3694583.1"
                     /translation="MTLRVEWMRGCATALVTPFGADGEVDEARVRALAERQVAGGVRV
                     LVPCGTTGESVTMSEEEDQLVIRTAIEAAHARGAHVIAGASSNSTAMAVRYSQAARDA
                     GADAILSVAPYYNKPTQDGLYAHFRTIAESIAPLPVVLYNVPGRTSSNIAAPTVLRLA
                     REVENIVAVKEASGNLGQIMEILRGRPRDFRVLSGDDAMTLAMLALGADGLVSVASNE
                     APAEMSRMVEAALAGRWDEARELHYKLLPLMDVNFVETSPGPVKAAMKMMGLLEEELR
                     LPLVPVGEKTRARVREVLVELGLLEESANVAA"
BASE COUNT          255 a          546 c          617 g          257 t
ORIGIN      
        1 gcgcggagga gctggccgag attctcggcg gccacgacgc cgccatcgac ttcaccatcg
       61 ccgaggccgt cccgcgccac gccgaagcct gcgccctcgc gcgcgtaccc ctggtcgagg
      121 gcacgacggg ctggcacgcg caactcgaag acgtgcgtca gttgttcgag cgcgagaatg
      181 gtgcgctcat ctacggcgcg aatttctcgg tcggcgtcaa cctcttctac cgcgtcgccg
      241 cgcgcgcggc ggaactgttc gccgccgtcg aagggtacga ccccttcatc gaagaggcgc
      301 accactcgcg caagcgcgac gcgccttcgg gcacggcgct gcgtttgcgc gagatcgtcg
      361 cggagcacgc ggggcgcgaa gtccccatcg cctcgacgcg cgccggccac atccccggca
      421 cgcaccgcgt cggcttcgac tcggcggcgg acacggtcac gctcacgcac acggcgcgct
      481 cgcgcgaagg cttcgccgcc ggcgccgtcc tcgccgcgcg ctggatcgga gggcgccgcg
      541 gcgtctacga gttcgcggag atgttcgacg aaatacttaa aggccgtgaa tcgtgaaccg
      601 taaaccgtga cgagtaaaaa aggctctgtc gcttgtcgct cgttacttgt ttctgctttc
      661 cacttatcac ggttcacggt tcacggttca cggctttcgg aggtgagtga tgacgttgcg
      721 agttgaatgg atgaggggct gcgcgacggc gctggtgacc ccgttcgggg cggacggcga
      781 agttgacgag gcgcgtgtgc gcgcgttggc cgagaggcag gtggcgggcg gcgtgcgcgt
      841 gctcgtgccg tgcgggacga cgggcgagag cgtgacgatg tcggaggagg aggatcagct
      901 cgtcatccgc acggcgatcg aggccgcgca cgctcgcggc gcgcacgtca tcgcgggcgc
      961 gagcagcaac tcgacggcga tggccgtgcg ctactcgcag gcggcgcgcg acgcgggcgc
     1021 cgacgccatc ctcagcgtcg cgccctacta caacaagccg acgcaggacg gcctctacgc
     1081 gcacttccgc accatcgccg agtcgatcgc gccgctgccc gtcgtgctct acaacgtgcc
     1141 ggggcgcacg tcttcgaaca tcgcggcgcc gaccgtcctc aggctcgcgc gcgaggtcga
     1201 gaacatcgtc gccgtcaaag aggcttcggg caacctcggg cagatcatgg agattctgcg
     1261 cgggcggccc cgcgacttcc gcgtgctgtc gggcgacgac gcgatgacgc tggcgatgct
     1321 cgcgctcggc gcggacgggc tggtctcggt ggcgtcgaac gaggcgcccg cggagatgtc
     1381 gcggatggtc gaggcagcgc tcgcaggtcg ctgggacgag gcgcgcgagc tgcactacaa
     1441 gctgctgccg ctgatggacg tgaacttcgt cgagacgagt cccggccccg tgaaggcggc
     1501 gatgaagatg atgggcctgc tggaagaaga gctgcggctg ccgctcgtgc ccgtcggcga
     1561 gaagacgcgc gcgcgcgtgc gcgaggtgct ggtcgagctg ggcctgttgg aggagagcgc
     1621 gaatgtcgct gcgtgagcgc gtggaggcgc tggccgcgaa gggggcgggc gcctt
//
LOCUS       DBNGOH010000193        11284 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1494769, whole genome shotgun sequence.
ACCESSION   DBNGOH010000193 DBNGOH010000000
VERSION     DBNGOH010000193.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 11284)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11284)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11284
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1494769"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(8..370)
                     /locus_tag="AC159P_03675"
     CDS             complement(8..370)
                     /locus_tag="AC159P_03675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3694584.1"
                     /translation="MNRRVLAAVDDLFFASKIRGTAEQLNVTVEFAKKAATLFDLAKA
                     ETPDLIILDLNSERLDPLGLAKDIRADEQLRSVHLVGFLSHVQTDLMRLAQDAGVDKV
                     LPRSAFTKFLPRILTGES"
     gene            425..1408
                     /locus_tag="AC159P_03680"
     CDS             425..1408
                     /locus_tag="AC159P_03680"
                     /inference="COORDINATES: protein motif:HMM:NF015338.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HIR3694585.1"
                     /translation="MFHAKPSTTNDEPPRGKLSHAHDIEAGEFAPPDAREIDRTKLVV
                     ATYNIRYAVGSLLITGSLFRRLGISRPARRPALVGGNIEKAARVFKGERRMPPADVIA
                     LQEADRGTERAGRRHVARELAAALGMNYVRASVPTPDEEDHKKKQWYLDFEERIRRGE
                     EGDTGVAILSRLALAGAARVELPWVECPWRPRLAMTARVPVAGRWLHVFNAHIDPHAN
                     LPEQLKQHEAVLDHADDACAGEPVALVGDFNTLIPRARDAARALLESRGFTSPMPSGT
                     GTWRAGLLRLHADWIFVRGVRVTRWGVARGAGISDHWPVWAELDAGGAVRA"
     gene            1405..2214
                     /locus_tag="AC159P_03685"
     CDS             1405..2214
                     /locus_tag="AC159P_03685"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012797.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HIR3694586.1"
                     /translation="MSEAVIKSRGNPAVRRARAVRDGRDRDFVFVEGVRLCEEALGAS
                     LDIETVFHAESLREDERGSRLLASLERAGGRLLAITEDVLDFVSDTKTSQGVVALARR
                     PRATEDAFEPLAGVPLVVVLHRVSNPANAGAMLRVAEAAGATGVVATAGTTDLLSPKA
                     LRGSMGSAFRLPLWTGAALEEVLRWCAARGIRTVATASDAPRAHTEVDWKTTRAVVVG
                     PEASGLSTEELAAADERVRVPMRAGVESLNAAVALGVVLYEAARQRGFAWK"
     gene            2458..3303
                     /locus_tag="AC159P_03690"
     CDS             2458..3303
                     /locus_tag="AC159P_03690"
                     /inference="COORDINATES: protein motif:HMM:NF028007.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HIR3694587.1"
                     /translation="MSQDKEKEKDKEKKRDEALVERLFRRVFEGVGSVVDRRLGREPE
                     AQTGFTTSKLIERMKRLIDERARDDDRGGRIAPHLLKLKIEWGTHSEAAPEYLRELEH
                     EILASAIDHINDQRLRTLAPVKIETSVDLFTTGIAVDPTYGEFEEELKQKDEEARRAK
                     ELDIHIPTSVPGAAATRDVTVLARVTLPGASHEARLSFKPGGRRMNVGRVSDNDLQLN
                     HPSVSKIHAALVMNAEGTLLVADTGSTNGTFINGRRISYGEARQLVDGDVVGFGDAEV
                     RFKKT"
     gene            3436..5382
                     /locus_tag="AC159P_03695"
     CDS             3436..5382
                     /locus_tag="AC159P_03695"
                     /inference="COORDINATES: protein motif:HMM:NF013100.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HIR3694588.1"
                     /translation="MFLLLATPEKQPALLSIVYTAGLALLGLLLLVSLLRQMRRRHSA
                     LAAVAPADLPPEVRDRLGTTSADRGLRALRVVFVLLALTVFGFHVYWAKYAEESNDRF
                     QQLSYKDLRNRRLAESTLRGWILDRTGSLDRALAHYKRAPNGQINREYPLDMEMAQLF
                     GSDRGDPGLERALFGVQSGAAPEAFDVVMEREVKQPGNIDVRLTIDSELQKTAVELLK
                     GRHGAVVVLNPQTGEVLAMYSNPAYSLKEAQDEAAYIRLEANDRDKPLVNRALGAYYI
                     PGSTFKTVTMLAAFNAGMQNEQFNCTGAGYYAEPGAKPIFDAGGAGEVHGRIGIDTAY
                     EVSCNQYFAQMAIKLGPERMADGARLIGITPYTNTTDAIRGRKQPQIWNTSLPSVARA
                     LAPREATIAVWPGMRRFDLGLVGYGQGYSGQMTPFQMALAASAIANLEGKLMKPKIEY
                     DVPPAAFAQATSPQHAAEMRRIMGLVSGGSSGTATRVMARVHAAGIITGGKTGTAEKE
                     VPVYDPKTGEEKRVKKIERDRRGNPIRETYEIVLSPEMRIDSWFLCIAPLDRPQLAIA
                     VIVEGGGYGATAAAPIAADLVLKARELGLLGQPAAAPASQGQPQGQPQQRQTPQPRSS
                     PRPRASPATAAPRANGQRAQPTGR"
     gene            5443..7146
                     /locus_tag="AC159P_03700"
     CDS             5443..7146
                     /locus_tag="AC159P_03700"
                     /inference="COORDINATES: protein motif:HMM:NF013279.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0015648 - lipid-linked peptidoglycan
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HIR3694589.1"
                     /translation="MKNRASSHLLLLLLIVGIEIVGYYAVYQSALLRGYEPSIVSAVR
                     DLLLYVPIIGILFWLSRAMRFRGNWALYTTAVLLFSVGMLVQYRLYSDPEYGSGNKAA
                     ARAEKMQVQRMRFISRYYDAEKKRLIGVEATAPQPDAPPQISRRSSVTVGQVLVSSFT
                     WIPLFSFFAMCAAFWVCTQDRWLNWMQRHSFVIVLVTLIPLGLAVASSSAGKFLGDMT
                     PWEPSKIPFLIGFAGILTAHYRELARTYWGMPRAQNVLPLVIMGLIPFIPFFALKDFG
                     QMLVFSGAYATLYLVAVRRWPQLFVFVGSLVLVVAVLVAGALPPNVQEKVPTLTTLAA
                     PVQTVLPARIKQRFHLWLDGFNPPSPEMEWWKKDYDEALAGNDRMRQLAEESPAMQRT
                     VNVDVWFDRIAFQPSQATFGIAAGGTSGRGLGLGFAEVIPVADSDYIYAAIGEELGLL
                     GGAVVILALCVFVSAGVRTSIEARDMFTKLCAAGLTAFIGFQALVNIGGTTRALPMTG
                     ITLPFVSHGGFSLITSFAMLGKLLAFSHRNARDAEALPRTAPSAAKRAHSDIKIPEAV
                     Q"
     gene            7143..8711
                     /locus_tag="AC159P_03705"
     CDS             7143..8711
                     /locus_tag="AC159P_03705"
                     /inference="COORDINATES: protein motif:HMM:NF025053.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HIR3694590.1"
                     /translation="MTEPSPRIRVEVASVTDRGMNEKRPLNQDSMLSDEEHGIFAVAD
                     GVGGGQAGEVASETAVEVLQQAFVHHQEGDDIEDLMEIAIQRANASIYQMSREDPRFA
                     MMATTIVALHLDGLRATVGHVGDSRLYRLTPDGQLQRETDDHSVVEEEVRAGRMTAQQ
                     AANHPSRNVISRALGAEQAVEVDMKTFEFDEGTTFLLCSDGITRHIPDEELVALLRNA
                     SDLENVCAEMKRLCYERGAEDNLTAIIVRVGAPASLSAPAPAEVDGEFDEEQTLIRRR
                     AAFADVPTAPLEPAPPSLLQRPFAGAGDDASPAAASFVPARDSASTSHDAQAAAPARQ
                     QRGGGGGRVIVGLLLLAAVAAAAFYGGLRFGRGGGLTGAATTASPVASPSATPTPESA
                     DERFDRLRRRVDLAPAIEAARIETETGNQPLNSTDPEQLYLYGRAMLLTNKSREAVSA
                     FEKTIERIKENETARNGQLRIDTRLAEIAANLRSEDFNKARDAAKALGDEVVRPAGTE
                     APANTSPSPAVTPF"
     gene            8788..10341
                     /locus_tag="AC159P_03710"
     CDS             8788..10341
                     /locus_tag="AC159P_03710"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR3694591.1"
                     /translation="MSELKLQQCRLDGRYDVLDCLGRGSYSEVYVARDIAASNGSPQT
                     VVIKALNTSLQGMPDPELERTLIENFRNEAVALDRVRHPHIISRLGHGTAIDLQNRTF
                     HYLVFEYLPGGDLQALCRQRPLSLEAALHYLRQVCAGLAHAHRQGVIHRDIKPQNLLL
                     TADQRTVKIADFGVAKIEADEGLITRVGTDVYAAPEHHPLMQTGRLDTASLTHPIVHL
                     TPAADIYSLAKTTYMLLSGESPRRFSQKPITALPVALARQPWAAYVSHVLERATRTNP
                     ERRHQTVEEFWHDIKDATMARTVPLGAAAPEPQPAPLEQHEEPRAAPPPTPTPPAPHF
                     ERNVEPSRTSTRQRIVVPIAEAAQLERARAEQRGGLGASARERRRAEPAVYDVPEESR
                     LSPRTRNVLVALFLLSAFALMLYATHYYVKMRSAPGTQTQQQRQQAGADASLTGKRFK
                     TTTDVNLRSGPSRSFGEVGRAELNSRVRVLQVNGKWYEVEVLEHGREKEDPSSADRGW
                     AHSAYLRPE"
     gene            10372..11235
                     /locus_tag="AC159P_03715"
     CDS             10372..11235
                     /locus_tag="AC159P_03715"
                     /inference="COORDINATES: protein motif:HMM:NF023818.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FhaA domain-containing protein"
                     /protein_id="HIR3694592.1"
                     /translation="MGIIESIRKWIDGEVTDDPLAEVDAEARPRRVWEEFLVKVAREI
                     EAVMQREMFTPPGGPTYIPPEYVVYLSSDDDKEWQGEKRRGLEQGLFYVLSERARELS
                     GATQLATKSFAVELRVDGTLDKGQFRVQPVWDAAEGGHTMVTPRPSEIAAASAATGAQ
                     ASEEGHADSELPESEVTKVTIRPAELYSVEVWRNAVRQTVVPVLKQEITIGRGSKSTT
                     VDLPIKGDPDVSRLHAHLERDNEGRYWFTPKGRNPTLVGGREVQREERVEVRPDDKIE
                     IASYTLRIQPK"
BASE COUNT         1891 a         3990 c         3715 g         1688 t
ORIGIN      
        1 gcttcgctca agactcgccc gttaaaatcc gaggcagaaa cttcgtgaac gccgagcgcg
       61 gcaagacctt gtcaaccccc gcgtcctgcg cgaggcgcat caggtcggtc tgcacgtgcg
      121 agaggaagcc gacgaggtgg acggagcgca gttgctcgtc cgccctgatg tctttagcga
      181 gtccgagcgg gtcgagcctt tcggagttga ggtcgaggat gatgaggtcg ggcgtctccg
      241 ccttggcgag gtcgaagagc gtcgcggctt ttttggcgaa ctcgaccgtg acgttcagtt
      301 gctccgccgt cccgcggatt ttcgacgcga agaagaggtc gtctacggcg gcaagcacgc
      361 ggcgattcat ttataatccc tctcgattcg taatttgaag acgcggccat tgtgcacccg
      421 ccggatgttc cacgcaaagc cttcgaccac gaacgacgag ccgccgcgag gcaagctctc
      481 gcacgcgcac gacatcgaag cgggcgagtt cgccccgccc gacgcgcgcg aaatcgatcg
      541 gacgaagctc gtcgtcgcca cttacaacat tcgctacgcc gtcggctcgc tgctcatcac
      601 gggcagtctc ttccgccgcc tcggcatcag caggcccgcg cgccggcccg cgctcgtcgg
      661 cggtaacatc gaaaaggcgg cgcgcgtttt caaaggcgag cggcggatgc cgcccgccga
      721 cgtgatcgcg ttgcaggagg ccgacagggg gacggagcgc gcgggccgtc ggcacgtggc
      781 gcgcgagctg gcggcggcgc tcgggatgaa ttacgtgcgc gcctccgtgc cgacgccgga
      841 cgaagaggat cacaagaaga aacagtggta tctcgatttc gaggagcgca tccgccgcgg
      901 cgaagagggc gacacgggcg tcgccatcct gagccggctc gcgctcgcgg gcgcggcgcg
      961 cgtcgagctg ccgtgggtcg agtgcccctg gcgaccgcgt ctcgcgatga cggcgcgcgt
     1021 ccccgtagcg ggcaggtggc tgcacgtctt caacgcgcac atcgacccgc acgcgaacct
     1081 gccggagcag ttgaagcagc acgaggccgt gctcgaccac gcggacgacg cgtgcgcggg
     1141 agagccggtg gcgctcgtcg gcgacttcaa cacgctcatc ccgcgcgcgc gcgacgcggc
     1201 ccgcgccctg ctcgaatcgc gcggcttcac ttcgccgatg ccttccggca cgggcacctg
     1261 gcgcgcgggc ctgttgcgtc ttcacgccga ttggattttc gtccgcggcg tgcgcgtcac
     1321 gcgctgggga gtcgcgcgcg gcgcgggcat ctccgaccac tggccggtct gggccgagct
     1381 ggacgcggga ggagccgtgc gcgcgtgagc gaggccgtca tcaagagccg cggcaaccct
     1441 gccgtccgcc gcgcgcgtgc ggtgcgcgac ggcagggaca gggatttcgt tttcgtcgaa
     1501 ggcgtcaggc tgtgcgagga ggcgctgggc gcttcgctcg acatcgagac ggtctttcac
     1561 gccgagtctt taagagaaga cgagcgcggg tcgcgactgc ttgcgtctct cgaacgggcg
     1621 ggcggtcgtc tgctggcgat cacggaggac gtgctcgact tcgtctccga cacgaagact
     1681 tcgcagggcg tcgtcgcgct ggcccgccga ccgcgcgcga ccgaagacgc gttcgagccg
     1741 ctggcgggag tgccgctcgt cgtcgtcctg caccgcgtaa gcaaccctgc gaacgcgggc
     1801 gcgatgctgc gcgtggccga ggcggcgggc gcgaccggcg tcgtcgccac cgcgggcacg
     1861 accgacctgc tgtcgccgaa ggcgctgcgc ggctcgatgg gttcggcctt ccgcctgccg
     1921 ctctggacgg gcgcagcact cgaagaagtg ctgcgctggt gcgccgcgcg cggcatccga
     1981 acggtcgcca ccgcgtcgga cgcgccgcgc gcgcacaccg aggttgactg gaagaccacg
     2041 cgcgccgtcg tcgtcggccc cgaagcgagc ggcctctcga cggaagaact cgcagcggcg
     2101 gacgagcgcg tgcgcgtccc gatgcgcgcg ggggtcgaga gcctcaacgc cgcggtcgcc
     2161 ctcggcgtcg tcctctacga agccgcgcgc cagcgtgggt tcgcatggaa gtagaaaggt
     2221 caaagcaaga agcgaacgtg tgggcaaagc ttcgctttgt cttttttatt ttctcgaagt
     2281 cgtgccgccg ccatgtccgg aacttttccc tttttacttt tcccttttta ctttcatata
     2341 atgccgccag tgaaacttca aagcatctcg gttcccctcc cccttgatct aactttcgtc
     2401 gcgccggtgt gagcttgcgg cgcccgaggc cgggattttt cgtgccccga aaatctgatg
     2461 agtcaggaca aagagaaaga gaaggacaag gagaagaagc gggacgaggc gctggtcgag
     2521 cgcctgttcc ggcgcgtctt cgagggcgtg ggatcggtcg ttgaccgccg gctcgggcgc
     2581 gagccggagg cgcagaccgg cttcaccacc agcaagctca tcgagcgcat gaagcggctc
     2641 atcgacgagc gcgcgcgcga cgacgaccgc ggcgggcgca tcgccccgca cctgctgaag
     2701 ctcaagatcg agtggggcac gcactccgag gcggcgcccg aatacctccg cgagcttgag
     2761 cacgaaatct tggcctccgc catcgaccat attaacgacc agcgcctgcg cacgctcgcg
     2821 cccgtcaaga tcgagacgag cgtcgatctc ttcacgacgg gcatcgccgt cgatccgacc
     2881 tacggcgagt tcgaggagga gctgaagcag aaggacgagg aggcgcgccg cgccaaagaa
     2941 ctcgacatcc acatccccac gtccgtgccg ggcgccgccg ccacgcgcga cgtgacggtg
     3001 ctggcgcgcg tgacgctgcc cggcgcctcg cacgaggcac ggctctcttt caagcccggc
     3061 gggcgccgga tgaacgtcgg gcgcgtctcg gacaacgacc tccaactcaa ccaccccagc
     3121 gtctcgaaga ttcacgccgc gctcgtgatg aacgcggagg gcacgctcct cgtcgccgac
     3181 accggctcga ccaacggcac cttcatcaac ggtcgtcgca tctcctacgg cgaggcacgt
     3241 cagctcgtgg acggcgacgt ggtcggcttc ggcgacgcgg aggtgagatt caaaaagacg
     3301 tgagccgtga accgcgaacc gtgacaagtc aagaacgtat ttcttcttgt cgcttccgcg
     3361 gctgacggtt cacggcgcac ggttcacgat ccgcgttctt acttgtcacg gtttacggtt
     3421 tacggttcac gtcttatgtt tttactgctg gcaacacccg aaaagcagcc ggcgctgctg
     3481 agcatcgtgt acacggcggg gctggcgctg ctgggcctgc tgctgttggt gtctttgctg
     3541 cggcagatgc ggcggcggca ttcggcgttg gcggcggtgg cgcccgcgga cttgccgccc
     3601 gaggtgcgcg accgcctcgg cacgacctcg gcggatcgcg gcctgcgcgc gctgcgcgtc
     3661 gtcttcgtgc tgctggcgct caccgtcttc ggcttccacg tctactgggc gaagtacgcc
     3721 gaggagtcga acgaccgctt ccagcagttg agttataaag acctgcgcaa ccggcggctg
     3781 gccgaatcga cgttgcgcgg ctggatactc gaccgtacgg ggagcctcga ccgcgcgctc
     3841 gcgcactaca agcgcgcgcc caacgggcag atcaaccgcg agtacccgct cgacatggag
     3901 atggcgcagc tcttcggctc cgaccgcggc gaccccggcc tcgaacgcgc gctcttcggc
     3961 gtccagagcg gcgccgcgcc cgaggcgttc gacgtggtga tggagcgcga ggtgaagcag
     4021 cccggcaaca tcgacgtgcg cctcaccatc gacagcgagt tgcagaagac ggcggtcgag
     4081 ctgctgaagg ggcgtcacgg cgcggtcgtc gtgctcaacc cgcagaccgg cgaggtgctc
     4141 gccatgtaca gcaaccccgc ctacagtttg aaggaagcgc aggacgaggc cgcctacatc
     4201 cgtctcgaag cgaacgaccg cgacaagccg ctcgtcaacc gcgcgctcgg cgcctactac
     4261 atccccggct cgactttcaa gaccgtgacg atgctcgccg ccttcaacgc ggggatgcag
     4321 aacgagcagt tcaactgcac gggcgcgggc tactacgccg agccgggcgc caagcccatc
     4381 ttcgacgcgg gcggcgccgg cgaagtccac gggcgcatcg gcatcgacac ggcctacgag
     4441 gtctcgtgca accagtactt cgcgcagatg gcgatcaagc tcggccccga gcggatggcc
     4501 gacggcgcgc gcctcatcgg catcacgccc tataccaaca cgacggacgc gattcgtggt
     4561 cgcaagcagc cgcagatttg gaacacgagt ctgccgtcgg tcgcgcgcgc gctcgcgccg
     4621 cgcgaggcga ccatcgcggt ctggccgggg atgcgcaggt tcgacctcgg gctggtcggc
     4681 tacgggcagg gctactcggg gcagatgacg ccgttccaga tggcgcttgc ggcctctgcg
     4741 attgcgaacc tcgaaggcaa gctgatgaag ccgaagatcg agtacgacgt gccgcccgcc
     4801 gccttcgcgc aggccacctc gccgcagcac gcggcggaga tgcggcgcat catgggcctc
     4861 gtctcgggcg gctcctcggg cacggccacg cgcgtcatgg cgcgcgttca cgccgcgggc
     4921 atcatcacgg gcggcaagac cggcaccgcc gagaaggaag tccccgtcta cgacccgaag
     4981 accggcgagg agaagcgcgt caagaagatc gagcgcgacc ggcggggcaa cccgattcgc
     5041 gagacttacg aaatcgtcct ctcgcccgag atgcggatcg acagttggtt tctctgcatc
     5101 gcgccgctcg acaggccgca gctcgccatc gccgtcatcg tcgagggcgg cggctacggc
     5161 gcgaccgccg ccgcgcccat cgccgccgac ctcgtgctca aggcgcgcga gttggggctg
     5221 ctcgggcagc ccgccgcggc ccccgcgtcg caaggtcagc cgcagggcca gccgcagcaa
     5281 cggcagacgc cgcagccgcg ctcttcgccg aggccgcgcg catcgcccgc cacggcggcc
     5341 ccgcgcgcca acggccagcg ggcgcagccg acggggcgtt gaggaggagg cggcttcgcg
     5401 tgcggttccc aatggtcgtg ctccagagtt gattcaagcg ttatgaaaaa cagggcttcg
     5461 tcacacctcc tgctcctgct gctgatcgtc ggcatcgaga tcgtcggcta ctacgcggtc
     5521 taccagtcgg cgctgctgcg cgggtacgag ccgtcgatag tcagcgccgt gcgcgacctc
     5581 ctgctgtacg tgcccatcat cggcatcctg ttctggctct cgcgcgcgat gaggttccgc
     5641 ggcaactggg cgctctacac gacggcggtg ctgctcttct cggtcgggat gctcgtccag
     5701 taccggctct acagcgaccc ggagtacggc tcgggcaaca aggccgccgc gcgcgcagag
     5761 aagatgcagg tgcagcggat gcgcttcatc agccggtatt acgacgccga gaagaagcgg
     5821 ctcatcggcg tcgaagcgac cgcgccgcag cccgacgcgc cgccgcagat ttcgcgccgt
     5881 tcgagcgtca ccgtcgggca ggtgctcgtg tcgagcttca cgtggattcc gctcttctcc
     5941 ttcttcgcca tgtgcgcggc cttctgggtc tgcacgcagg atcgttggct gaactggatg
     6001 cagcgccaca gcttcgtcat cgtgctcgtg acgctgatcc cgctgggcct ggccgtcgcc
     6061 tcttcgagcg cgggcaagtt cctcggcgac atgacgccgt gggagccttc gaagattccc
     6121 ttcctcatcg gcttcgccgg catcctgacg gcgcactacc gcgagctggc gcgcacctac
     6181 tggggcatgc cgcgcgcgca gaacgtgctg ccgctcgtca tcatggggct gatcccgttc
     6241 atccccttct tcgcgctcaa ggacttcggg cagatgctcg tcttcagcgg cgcctacgcg
     6301 acgctctacc tcgtcgccgt gcgccgctgg ccgcagctct tcgtcttcgt cggctcgctg
     6361 gtgctcgtcg tcgcggtgct cgtcgccggg gcgctgccgc ccaacgtgca ggagaaggtg
     6421 ccgaccctga cgacgctggc cgcgcccgtc cagaccgttt tgcccgcgcg catcaagcag
     6481 cgcttccacc tctggctcga cggcttcaac ccgccctcgc ccgagatgga gtggtggaag
     6541 aaggactacg acgaagcgct cgcgggcaac gaccgcatgc ggcagctcgc cgaggaaagc
     6601 cccgcgatgc agcgcaccgt gaacgtggac gtgtggttcg accgcatcgc gttccagccg
     6661 tcgcaggcca ccttcggcat cgcggcgggc ggcacgagcg ggcgcggcct cggcctcggc
     6721 ttcgccgagg tcatcccggt cgccgactcc gactacatct acgccgccat cggcgaagag
     6781 ctgggcctgc tcggcggcgc ggtcgtcatc ctcgcgctgt gcgtcttcgt ctcggcgggc
     6841 gtgcgcacct ccatcgaggc gcgcgacatg ttcaccaagc tctgcgcggc gggcctgacg
     6901 gcctttatcg gctttcaggc gctcgtcaac atcggcggca ccacgcgcgc gctgccgatg
     6961 acgggcatca ctttaccttt cgtcagccac ggcggcttca gcctcatcac gagcttcgcc
     7021 atgctcggca agctgctcgc gttctcgcac cgcaacgcgc gcgacgccga ggcgctgccg
     7081 cgcaccgccc cctctgcggc gaagcgcgcc cacagcgaca tcaaaattcc ggaggccgtc
     7141 caatgacaga accttccccg aggattcgcg tcgaggtcgc cagcgtcacc gaccgcggca
     7201 tgaacgagaa gcgccccctc aaccaggact cgatgttgag cgacgaggag cacggcatct
     7261 tcgccgtcgc cgacggcgtc ggcggcggcc aggcgggcga ggtcgcctcc gaaaccgcgg
     7321 tcgaagtgct ccagcaggcg ttcgtccacc accaggaggg cgacgacatc gaagacctga
     7381 tggagatcgc catccagcgc gccaacgcct ccatttacca gatgtcgcgc gaggacccgc
     7441 gcttcgccat gatggcgacg accatcgtcg cgcttcacct cgacggcctg cgcgcgaccg
     7501 tcgggcacgt cggcgactcg cggctctacc gcctgacgcc cgacggccaa ctgcaacgcg
     7561 agacggacga ccactcggtc gtcgaggagg aggtgcgcgc cgggcgcatg acggcgcagc
     7621 aggccgccaa ccacccgagc cgcaacgtga tcagccgcgc gctcggcgcc gagcaggccg
     7681 tcgaagtgga catgaagacc ttcgagttcg atgagggcac gacgttcctc ctctgctccg
     7741 acggcatcac gcgccacatc cccgacgagg agctggtcgc gctgctcagg aacgcctccg
     7801 acctcgaaaa cgtctgcgcc gagatgaagc gcctctgtta cgagcgcggc gccgaagaca
     7861 acctgacggc catcatcgtc cgcgtcggcg cccccgcgtc gctctccgcc cccgcgcccg
     7921 ccgaagtgga cggggagttc gacgaagagc agaccctcat ccgccggcgc gcggcgttcg
     7981 cggacgtgcc gaccgcccct ttggaaccgg cgcccccctc cctgctccaa cgccccttcg
     8041 cgggggccgg cgatgacgcc tcgcccgccg ccgcgtcctt cgtgcccgcg cgcgactccg
     8101 cttcgacctc gcacgacgcg caggccgccg cgcccgcgcg ccagcagcgc ggaggcgggg
     8161 gcgggcgcgt catcgtgggg ctgctgctgt tggcggcggt ggcggcggct gcgttctacg
     8221 gagggctgcg tttcggtcgc ggcggaggac tcacgggcgc ggcgacgacc gcctcgcccg
     8281 tcgcttcccc ctcggcgacg ccgacgcccg agagcgccga cgagcgtttc gaccgcctgc
     8341 gtcgccgggt cgatctcgcg cccgccatcg aggccgcgcg catcgagacc gagaccggca
     8401 accaaccctt gaactcgacc gacccggaac agctctacct ctacggtcgc gcgatgctgc
     8461 tgacgaacaa gagccgcgag gcggtgtcgg cgttcgagaa gaccatcgag agaatcaaag
     8521 agaacgagac cgcgcgcaac ggccaactgc gcatcgacac gcggctcgcg gagatcgccg
     8581 ccaatctgcg ctccgaagac ttcaacaaag cgcgcgacgc ggcgaaggcg ctcggcgacg
     8641 aagtggtcag gcccgcgggc acggaggcgc cggcgaacac ttcgccctcc ccagccgtaa
     8701 caccatttta agaagtcgga aggctgaagg ctgaagtcag aattaaaaaa ccaaatgctt
     8761 ccattccgac ttccgacttg gatttcgatg agcgaactca aactccaaca gtgcaggctc
     8821 gacggtcgct acgacgtgct cgactgcctg gggcgcggca gctactcgga ggtctacgtg
     8881 gcccgcgaca tcgcggcctc gaacggctcg ccgcagacgg tcgtcatcaa ggcgctcaac
     8941 acaagcctcc aggggatgcc cgacccggag cttgagcgca cgctcatcga gaacttccgc
     9001 aacgaggccg tcgcgctcga ccgcgtgcgc cacccgcaca tcatcagccg cctcggccac
     9061 ggcaccgcca tcgacttgca gaaccgcacc ttccactacc tcgtcttcga gtacctgccc
     9121 ggcggcgacc tgcaagcgct ctgccgccag cgcccgctct cgctcgaagc cgcgctccat
     9181 tatttgcggc aggtctgcgc cggacttgcc cacgcgcacc ggcagggcgt catccaccgc
     9241 gacatcaagc cgcagaacct gctcctgacc gccgaccagc gcaccgtcaa gatcgcggac
     9301 ttcggcgtcg ccaagatcga ggccgacgaa ggattaatca cgcgcgtcgg cacggacgtg
     9361 tacgcggcgc ccgagcacca cccgctgatg cagaccggca ggctcgacac ggcctcgctg
     9421 acgcacccca tcgtccacct caccccggcg gctgacatct actcgctggc caagacgacc
     9481 tacatgctcc tctcgggcga gtcgccgcgc cgcttctcgc agaagccgat cacggcgctg
     9541 cccgtcgcgc tcgcgcgcca gccgtgggcc gcctacgtct cgcacgtttt ggaacgcgcg
     9601 acgcgcacca accccgagcg ccgccaccag acggtcgaag agttctggca cgacatcaag
     9661 gacgcgacga tggcgcgcac cgtcccgctc ggcgccgccg cccccgagcc gcagcccgcc
     9721 ccgctcgaac agcacgaaga gccgcgcgcc gcgccgccgc ccacgccgac cccgcccgcc
     9781 ccgcacttcg agcgcaacgt cgagccttcg cgcacctcta cgcgccagcg catcgtcgtc
     9841 cccatcgccg aggcggcgca gctcgaacgc gcgcgcgccg agcagagggg cggcctcggc
     9901 gcgtccgcgc gcgagcgccg ccgcgccgag ccggcagtct acgacgtgcc cgaagagtcg
     9961 cgcctgtcgc cgcgcacccg caacgtgctc gtcgcgctct tcctgctctc ggccttcgcg
    10021 ctgatgcttt acgccacgca ctattacgtt aaaatgcgct ccgcccccgg cacgcagacg
    10081 cagcaacagc ggcagcaggc gggcgccgac gcttcgctca ccggcaagag gttcaagacg
    10141 acgaccgacg tgaacctgcg ctccggcccg tcgcgcagct tcggcgaggt cgggagggcc
    10201 gagcttaact cgcgtgtgcg cgtgcttcag gtcaacggca agtggtacga ggtcgaggtc
    10261 ttggagcacg ggcgcgagaa ggaagacccg tcttctgccg accgcggctg ggcgcacagc
    10321 gcttatctca gaccggagtg agtggccggg tgaaaacgag gtgagagacc agtgggcata
    10381 atcgaatcga tccgcaaatg gattgacggc gaggtgacgg acgacccgct cgccgaggtg
    10441 gacgccgagg cgcggccccg ccgcgtctgg gaggagttcc tcgtcaaggt cgcgcgcgag
    10501 atcgaggccg tcatgcagcg cgagatgttc acgccgcccg gcggcccgac ctacatcccg
    10561 cccgagtacg tcgtctactt aagctcagac gacgacaagg agtggcaggg cgagaagcgc
    10621 cgcgggctgg aacagggact cttctacgtg ctctccgagc gcgcgcgcga gctttcgggc
    10681 gcgacgcagc tcgccaccaa gtccttcgcg gtggagcttc gggtggacgg cacgctcgac
    10741 aagggccagt tccgcgtcca gcccgtctgg gacgccgccg agggcggcca cacgatggtc
    10801 acgccgcgcc cctccgagat cgccgcggcg tccgccgcga ccggcgcgca ggcgtccgag
    10861 gagggccacg cggactcgga gttgcccgag agcgaggtca cgaaagtcac catccgcccc
    10921 gccgagctgt acagcgtcga ggtctggcgc aacgccgtcc ggcagaccgt cgtccccgtc
    10981 ctcaaacagg agatcaccat cggtcgcggc tccaagtcca cgaccgtaga cctgcccatc
    11041 aaaggcgacc ccgacgtcag ccgcctccac gcccacctcg aacgcgacaa cgaaggccgc
    11101 tactggttca cccccaaggg ccgcaacccc accctcgtcg gcgggcgcga ggtgcaacgc
    11161 gaagagcgcg tcgaagtgcg ccccgacgac aagatcgaga tcgccagcta caccctccgc
    11221 atccagccca agtaagaagg gggaaaaagt caaaagggga aagggcgaaa agggagagaa
    11281 gttg
//
LOCUS       DBNGOH010000194         6746 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2961705, whole genome shotgun sequence.
ACCESSION   DBNGOH010000194 DBNGOH010000000
VERSION     DBNGOH010000194.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6746)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6746)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6746
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2961705"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            86..1303
                     /locus_tag="AC159P_03720"
     CDS             86..1303
                     /locus_tag="AC159P_03720"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val family dehydrogenase"
                     /protein_id="HIR3694593.1"
                     /translation="MERFDRAAKILDLNPDLYAVLRVPNRELKVYIPVRMDSGRIEVF
                     EGYRVQHNFARGPAKGGIRYAPDVSIDEVRALAAWMTWKCAVVNVPFGGGKGGVVCDP
                     VQMSRGELERLTRRYAAELIDFIGPEKDVPAPDMNTNEQTMAWIMDTYSMHARHTVNA
                     IVTGKPVDLGGSRGRREATGRGLLFVINEAVKRFKMSPPDTRVVVQGSGNVGGIGARL
                     LHEAGYKVVAISDIFGGIHNADGIDIPAALDHLQKTRSFDEFPGVERVSNTELLELEC
                     DVLAPCATENQITSDNADRIKCRILAEGANGPTTAAADEILHNKGVFVIPDILANAGG
                     VTVSYFEWVQNRMGYYWTEDVVNERLQQVMVAGFKDVAKYAETHAVDTRTAAYMLAID
                     RVAYDTRMRGIYA"
     gene            1788..4238
                     /locus_tag="AC159P_03725"
     CDS             1788..4238
                     /locus_tag="AC159P_03725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694594.1"
                     /translation="MRLARRALFAALATTLLVSAVTAQTLRPENDPRNQAPTVGTGGT
                     PGGPTGLFTIYDGDTLRKGEYTFSIAYSNYDRDPGNVDITVVPLSFNIGINDYLELFF
                     STEGYRGVKVNNPANLSGFYLPNSQLFFGAGFANLRSPAAIILAPERVSGPTVGTGQV
                     FRPVGNQPFLQFPFVGGPGPNLGLTGNIIAPPFVSRIGAAQDQGGGTYGAAANFPGMG
                     SACGGILPGIVLTTRVIPANLTFNPITVPQLFTTCPTYIADAPFINRLYGESSFGTMH
                     LGGKFRFTEPDNPLGVGLVAFWRFYLDSADDQSGFNQLQRGASPGAGKFPGDWGVGGF
                     ISGRLSRSVNLSVNATYILNSNPKSEAFGGDDISLLDRPDELVSGIGFDFPVNKHFQP
                     IAELKSVWYVGGRTPNAFPNNPVDFLAGVRIFPRRWFGFSAWYRGHLNQQGDRILNDA
                     EFPTGFTPSRDPHGFGFQFFAGRRNERAPTIFPNQPPTVTLSASSSRVVLAADCTGGE
                     TPDPACTPTAPTVNLSATASDPDGDTLLYTYSTTGGRITGDGPNATLDLTGVAPGTYT
                     VTVEVDDGCGCVAFTSTTVTVERCPCEAIPPPPIACPTVRVSCPDTGTRGTPVTFTAE
                     VSSGDPAVTPTFNWTVSAGTISSGQGTSSITVDTTGITGPITATVDVGGYDRSCATSA
                     SCTTSFPPIPEARKIDEYGNIRFNDEKARLDNLAIELQNDPTAQGYLVCYGGRVGRAG
                     AAQARCDRAKNYLVTSRGLSADRIVTVDGGFREDLTVELWVVPQGAQPPAASPTVDPS
                     EVRTTRAPRRRGRRDDDE"
     gene            4551..>6746
                     /locus_tag="AC159P_03730"
     CDS             4551..>6746
                     /locus_tag="AC159P_03730"
                     /inference="COORDINATES: protein motif:HMM:NF026220.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HIR3694595.1"
                     /translation="MKKTFSLLLLFTLTISTSTVSFAVSPDDSDSEAAAQRLMGWEVT
                     GPMGGDVRSLVVDPQDPQRLYFGTIDGQIYTSADGGLMWERLSGFNRPGLLIDNIIVD
                     ARDSKTLYAAAHRHKEPGGFFKTTDGGKTWREAEAFKSEAIHSLTQSPSDPDTLLVGT
                     NRGIFRSTDAGDSWEPLPTSTTPGLINVESLAVDPRDANVIYAGTWYLPYKTTDGGKT
                     WGITKTGIIDDSDIFAIEIDASNPDHVIMSACSGIYETKNAGAQWRKVNGIPSQSRRT
                     RAILQNPAKPEIIYAGTTEGFWLSTNGGNDWKVTTNRQNFEVNAIAVHPKNPQTVYIG
                     TNNYGVMISRDGGRNFAPSNEGYSGRRAYSILPDREQPGRVYATTINTATGGGYFYIS
                     EDGGETWRLSARNMPYRLIAYSILQDPRDANTIYLGTNYGLYRSADRGATWAPMGAPK
                     PKAPARKRKGKAAAAAAEAVPEVPRPSADANVKRAQAALNVAGYEVGKPDGIAGTRTV
                     TALRKFQTDKRIPVTGQFDATTLTALGLGGGAQSAGAIADSLQTAPVYLTDTINALTY
                     DYKERDGQVGILAATNAGLFRSYDIAKGWERISFGADVDARTLCISTTAQNPETFYVG
                     TATSGVLVTRDGGKTWEQVRGIPTDAPVNVVEQDPKRSEYVYVGTTQTLYISHDGGQR
                     WQRRGGNLPLGSFTSILINPENPDEVFVGNAYERGGKVFTAAEGGGVFRS"
BASE COUNT         1241 a         2364 c         2099 g         1042 t
ORIGIN      
        1 tccccacttc tcggagatga ttatggctaa cccgatggtg gacgatacga ggcgggtgag
       61 ggaggagaac ccgttcgagt cgatgatgga gcggttcgac cgcgcggcga agattctcga
      121 cctcaacccg gacctgtatg cagtgctgcg cgtgccgaac agagagctga aagtctacat
      181 ccccgtcagg atggactcgg ggcggatcga ggtcttcgag ggctaccgcg tgcagcacaa
      241 cttcgcgcgc ggccccgcca agggcggcat ccgttacgcg cccgacgtgt cgatcgacga
      301 ggtgcgcgcg ctggcggcgt ggatgacctg gaagtgcgcg gtcgtgaacg tgccgttcgg
      361 cggcggcaag ggcggcgtcg tgtgcgaccc cgtccagatg tcgcggggcg agctggagcg
      421 cctgacgcgg cgctacgccg ccgagttgat cgacttcatc ggccccgaaa aagacgtgcc
      481 cgcgcccgac atgaacacca acgagcagac gatggcgtgg atcatggaca cctattccat
      541 gcacgcgcgc cacaccgtca acgccatcgt cacgggcaaa cccgtcgatc tcggcggctc
      601 gcgcgggcgg cgcgaggcca ccggaagagg cctgctcttc gtcatcaacg aggcggtcaa
      661 aaggttcaag atgtcgccgc ccgacacgcg cgtcgtcgtc caggggtcgg gcaacgtcgg
      721 gggcatcggc gcgcgcctcc tgcacgaggc gggctacaag gtcgtcgcca tctccgacat
      781 cttcggcggc atccacaacg ccgacggcat cgacatcccc gccgccctcg accacctgca
      841 aaaaacgcgc tcgttcgacg aattccccgg cgtcgagcgc gtctcgaaca cggagctact
      901 cgaactcgaa tgcgacgttt tggctccgtg cgcgaccgag aaccagatca cctcggacaa
      961 cgccgaccgc atcaagtgcc gcatcctggc cgagggcgcc aacgggccga ccactgccgc
     1021 cgccgatgaa atccttcata acaaaggcgt tttcgtcatc ccggacatcc tcgccaacgc
     1081 cgggggcgtg acggtctcgt acttcgagtg ggtgcagaac cggatgggct attactggac
     1141 cgaagacgtg gtcaacgaac gcttgcagca agtgatggtc gccggcttca aagacgtggc
     1201 caagtacgcc gagacgcacg cggtcgatac gcgcacggcg gcctacatgc tggcgattga
     1261 ccgcgtggcc tacgacacgc gcatgcgggg catctacgcg tgacaaaaaa gtgtaaagat
     1321 ttttcaccac cgggggcgaa aaagtcttga tttcagtgtg gatgtagagt atggtctggc
     1381 ggaaaatggt gcgccagagc cggagcgcgt ttagggtcat cggcgatgcg tctcgtggtg
     1441 ctccagacag atgtggttga cacggcgcat ctgtttccgt ataagcagag tagcgctaaa
     1501 gtcatacaga cagacacaag acaggcaaga gttctcagtt gagaggcgcc ggagaggcag
     1561 tgcaagagcg cccgcggaac gccggacagg tgttcctggc atctctcgct gcaccgctct
     1621 aatctacaag acatttcggt cgcgggcggt agcagtcttt actttgactt cagggtttgt
     1681 acgcttgtag ttggagcggc ttacagctct tctcacgcat tatccagaag aagtgccggg
     1741 tcgccccctt ccgcaaattt ataaactcat ttcgcaggag gaatgcgatg agactcgcga
     1801 gaagagcgct tttcgccgcg ctcgccacaa ctttactggt gtcggcagtg acggcccaaa
     1861 cgctcaggcc ggagaacgac ccgaggaacc aggctcctac cgtgggcacc ggcggcaccc
     1921 cgggtgggcc gaccggtctt ttcaccattt acgacggcga cacgctgcgt aagggtgaat
     1981 acaccttcag cattgcctac agcaactacg accgcgaccc cggcaatgtt gacatcaccg
     2041 tcgtcccgct cagcttcaac atcggcatca acgactacct ggagctgttc ttcagcacgg
     2101 aaggctaccg cggggtgaag gtcaacaacc cggcgaacct gtcgggcttc tacctgccga
     2161 actcgcagct cttcttcggt gcgggcttcg ccaacctgcg cagcccggcg gccatcatcc
     2221 tcgcgcccga gcgcgtgagc ggcccgaccg tcgggaccgg gcaggtcttc cgcccggtgg
     2281 gcaaccagcc gttcctgcaa ttcccgttcg tcggcggccc cgggccgaac ctcggcctga
     2341 cgggcaacat catcgcgccg ccgttcgtct cgcgcatcgg cgcggcgcag gatcagggcg
     2401 gcggcaccta cggcgccgcg gcgaacttcc ccggcatggg gtcggcctgc ggcggcatcc
     2461 tgcccggcat cgtgctgacg acccgcgtca tcccggccaa cctgaccttc aacccgatca
     2521 cggtgccgca actgttcacc acctgcccga cctacatcgc cgacgcgccc ttcatcaacc
     2581 ggctctacgg cgagtcgtcg ttcgggacga tgcacctcgg cggcaagttc aggttcaccg
     2641 agcctgacaa cccgctcggc gtcggcctcg tcgccttctg gcgcttctac ctcgatagcg
     2701 ccgacgacca gtcgggcttc aaccagcttc agcgcggcgc cagccccggc gcgggcaagt
     2761 tccccggcga ctggggcgtc ggcggcttca tcagcgggcg tctgagccgt tcggtcaacc
     2821 tgtcggtcaa cgccacctac atcctgaaca gcaacccgaa gtcggaagcc ttcggcggcg
     2881 acgacatctc gctgctcgac aggccggacg agttagtctc gggcatcggc ttcgacttcc
     2941 cggtgaacaa gcacttccag cccatcgccg agttgaagtc ggtgtggtac gtcggcgggc
     3001 gcacgccgaa cgcgttcccg aacaacccgg ttgacttttt ggccggcgtc cgcatcttcc
     3061 cgcgccgctg gttcggcttc agcgcgtggt atcgcgggca cctcaaccag cagggcgacc
     3121 gcatcctgaa cgatgcggag ttcccaaccg gattcacgcc gtcgcgcgac ccgcacggct
     3181 tcggcttcca gttcttcgcc ggtcgccgca acgagcgcgc cccgaccatc ttcccgaacc
     3241 agccgccgac cgtgaccctc tcggcctcgt cttcgagggt cgtgctcgcg gcagattgca
     3301 ccggcgggga gactcctgac ccggcctgca cgccgaccgc cccgaccgtc aacctgtcgg
     3361 cgacggcgag cgacccggac ggcgacacgc tgctctacac ctactcgacc acgggcgggc
     3421 gcatcacggg cgacggcccg aacgccacgc tcgacctgac gggcgtcgcg cccggcacct
     3481 acaccgtgac ggtggaagtg gacgacggct gcggctgcgt cgccttcacc tcgacgaccg
     3541 tgacggtcga gaggtgcccg tgcgaagcga tcccgccgcc gccgatcgcg tgcccgactg
     3601 tcagggtgag ctgccccgac accggcacca gaggcacgcc cgtcaccttc accgccgaag
     3661 tttcgagcgg cgacccggct gtcacgccga ccttcaactg gacggtctcc gccggcacca
     3721 tcagcagcgg ccagggcacg tcgtcgatca cggtcgatac gaccggtatc accggcccga
     3781 tcacggccac ggttgacgtg ggcggctacg accgtagctg cgcgacctcg gcgagctgca
     3841 cgacgagctt cccgccgatt ccagaggcac ggaagatcga cgagtacggg aacatccggt
     3901 tcaacgacga gaaggcgcgt ctcgacaacc tcgccatcga actgcaaaac gacccgaccg
     3961 cacagggcta cctcgtgtgc tacggcggca gggtcggacg tgcgggcgcg gcgcaggccc
     4021 gttgcgaccg cgcgaagaac tatctcgtca cctcgcgcgg cctctcggct gaccgtatcg
     4081 tgacggtgga cggcggcttc cgcgaagacc tgacggtcga actctgggtc gtgccgcagg
     4141 gcgctcaacc gcccgcggcc agcccgacgg ttgacccgag cgaggtcaga accacccgtg
     4201 ccccgcgccg ccgcggtcgc cgcgacgacg acgagtaagc ggacaacaca gctcgcgcgc
     4261 ccgatctgaa cgacgcgcga ccccttccga aagcgggcgg cgctccccac gaggagcgcc
     4321 gcccgcttcg cttttcgcgc cgcggcacgg ccctccgaga gtacaaccgg ccccaccgac
     4381 cccgcatcct acacacgcgg cgcggcgccc ctctccctgc cgaaattcgc gaacaaaacc
     4441 gtggcatgta tttttgcgaa caaaattgtg gtatgtatta ttgagtgcgg catttggccg
     4501 ccatgcccct ttgagcttga aactttgcac ctttgacgac ggaacgcctg atgaaaaaaa
     4561 ccttctcgct ccttctgctg ttcacgctga caatttccac ctcgaccgtt tcctttgccg
     4621 tctcccccga cgactcggac tccgaggccg cggcgcagcg cctgatgggc tgggaagtga
     4681 ccgggccgat gggcggcgac gtgcgctcgc tcgtcgtcga tccgcaagac ccgcagcgcc
     4741 tctacttcgg cactatcgac ggacagattt acacctcggc ggacggcggc ctgatgtggg
     4801 aacgcctgtc gggcttcaac cgccccggcc tcctgatcga caacatcatc gtcgatgcgc
     4861 gcgactcgaa gacgctctac gccgccgcgc accgccacaa ggagccgggc ggcttcttca
     4921 agaccaccga cggcggcaag acctggcgcg aggccgaggc gttcaagtcc gaggccatcc
     4981 actcgctcac gcagtcgcct tcggacccgg acacgctgct cgtcggcacc aaccgcggca
     5041 tcttccgttc gaccgacgcg ggcgactcgt gggagccgct cccgacatcg accacgcccg
     5101 gcctcatcaa cgtcgagtcg ctggcggttg acccgcgcga cgcgaacgtg atctacgcgg
     5161 gcacctggta tctgccgtac aagacgaccg acggcggcaa gacgtggggc atcacgaaga
     5221 ccggcatcat cgacgactcg gacatcttcg ccatcgagat cgacgcgagt aaccccgacc
     5281 acgtcatcat gtcggcgtgc agcggcatct acgagacgaa gaacgcgggc gcgcaatggc
     5341 gtaaagtcaa cggcatccct tcgcagtcgc gccgcacgcg cgccatcctc cagaacccgg
     5401 cgaagcccga gatcatctac gccggcacga ccgagggctt ctggctctcg accaacggcg
     5461 gcaacgactg gaaggtgacg accaaccgcc agaacttcga ggtcaacgcc atcgccgtcc
     5521 acccgaagaa cccgcagacg gtctacatcg gcacgaacaa ctacggcgtg atgatctcgc
     5581 gcgacggcgg tcgcaacttc gcgccgtcga acgagggcta ctcgggccgc cgcgcctact
     5641 cgatcctgcc cgaccgcgag cagccgggcc gcgtctacgc gaccaccatc aacacggcga
     5701 ccggcggcgg ctacttctac atcagcgagg acggcggcga gacctggcgc ctctcggcgc
     5761 gcaacatgcc ttaccgtctg atcgcctact cgatcctgca agacccgcgc gacgcgaaca
     5821 cgatctacct cggcacgaac tacggcctct accgctccgc cgaccgcggc gcgacgtggg
     5881 cgccgatggg cgcgccgaag ccgaaggccc cggcgcggaa gcggaagggc aaggccgccg
     5941 cggcggcggc tgaggcggtg cccgaagtgc cgcgcccctc ggcggacgcc aacgtgaagc
     6001 gcgcgcaggc cgctctgaac gtcgccggtt atgaagtcgg caagccggac ggcatcgcgg
     6061 gcacgcgcac cgtcaccgcg ctcaggaagt ttcagacgga caagcgcatc ccggtcacgg
     6121 gccagttcga cgcgacgacg ctgacggcgc tcggcctcgg cggcggcgcg cagtcggcgg
     6181 gcgccatcgc cgactctttg cagaccgcgc cggtttacct gaccgacacg atcaacgcgc
     6241 tcacctacga ctacaaggag cgcgacgggc aggtcggcat cctcgcggcc accaacgcgg
     6301 gactcttccg cagctacgac atcgcgaagg gctgggagcg catctctttc ggggcggacg
     6361 ttgacgcgcg cacgctctgt atctcgacga ccgcgcagaa cccggagacc ttctacgtcg
     6421 gcacggccac ctccggcgtg ctcgtcacgc gcgacggcgg caagacgtgg gagcaggtgc
     6481 gcggcatccc cacggacgcg cccgtcaacg tcgtcgagca agacccgaag cgctccgagt
     6541 acgtctacgt cggcacgacg cagacgctct acatctcgca cgacggcggg cagcgctggc
     6601 agcgccgcgg cggcaacctg ccgctgggga gcttcaccag catcctcatc aaccccgaga
     6661 accccgacga ggtcttcgtc ggcaacgcct acgagcgtgg cggcaaggtc tttacggcgg
     6721 ccgagggcgg cggcgtcttc cgctcg
//
LOCUS       DBNGOH010000195         1794 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2411886, whole genome shotgun sequence.
ACCESSION   DBNGOH010000195 DBNGOH010000000
VERSION     DBNGOH010000195.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1794)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1794)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1794
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2411886"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..629)
                     /locus_tag="AC159P_03735"
     CDS             complement(<1..629)
                     /locus_tag="AC159P_03735"
                     /EC_number="3.4.21.-"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HIR3694596.1"
                     /translation="MSEGRTREAEGYAGRPSVCRRCGALVGAGERDCMMCGAPVVREP
                     TDGERAARHPARDRETIRFIRAIISRPATFTFVFLTACVFLYLLMELSGGAQGAVLVE
                     YGAKVNRLIDERGEWWRFVTPIFLHVTVPGYGPLHLLTNMYGLFMLGPYVEKLYGSPK
                     FVFFWMLTGVAGVATSYLTVRPELAAGPWGYIFKARDYPAAGASGALFGL"
     gene            1052..1210
                     /locus_tag="AC159P_03740"
     CDS             1052..1210
                     /locus_tag="AC159P_03740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694597.1"
                     /translation="MPKKCIPVLLLAASLFAPLAGASTAPAPKTVLANAVPPLPLAAS
                     RIESELTH"
     gene            1314..>1794
                     /locus_tag="AC159P_03745"
     CDS             1314..>1794
                     /locus_tag="AC159P_03745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694598.1"
                     /translation="MKKRLTATLFTLLLATSALGLQAPASWTKFTSQAGRFSVMLPAE
                     PKEEKETKNDGGPLSPYTNTLYTAQGAGAVYLVGWTDYKPGVRINVPGEIKANRDNFV
                     NAIKATVATEKAIKLGTHPGTEFTAENAQAFFHVRVYVIGRRPYILVALRPKGTQDPN
                     "
BASE COUNT          330 a          641 c          538 g          285 t
ORIGIN      
        1 agcccgaaca gcgcgcccga ggcgcccgcc gccgggtagt cgcgcgcctt gaagatgtag
       61 ccccaaggcc ctgcggccag ctcggggcgc accgtcaggt agctcgtcgc gacgccggcc
      121 acgcccgtca gcatccagaa gaagacgaac ttcggcgagc cgtacagctt ctcgacgtac
      181 gggccgagca tgaacagccc gtacatgttc gtcagcaggt gcagcgggcc gtagccgggc
      241 accgtcacgt gcaggaagat cggcgtgacg aagcgccacc actcgccgcg ctcgtcgatc
      301 agccggttga ccttcgcgcc gtactcgacg agcaccgcgc cctgcgcgcc gcccgagagc
      361 tccatcagca gatagaggaa gacgcacgcc gtcaggaaga cgaaggtgaa cgtggcgggg
      421 cgcgagatga tggcgcggat gaagcggatc gtctcgcggt cgcgcgcggg gtgccgcgcc
      481 gcgcgctcgc cgtccgtcgg ctcgcgcacg accggggcgc cgcacatcat gcagtcgcgc
      541 tcgcccgccc cgaccagcgc gccgcaccgc cggcacacgc tcgggcgccc ggcatacccc
      601 tcggcctctc tcgttcgacc ttctgacaag actttgttgc gacgaaaaat tttacgcttg
      661 cgcgtgattc tacaacgccg cctcgcacac tgtcatgcag gagaagaagt ccggagtccg
      721 gagtctggag tcagaacaaa gaggttgagg atagcctttg gactttacct ctcaaactcc
      781 ctcattctcc tgacttcaga ctccagactc cctcccctcc tcgttcgcct tgacggaaat
      841 cgtgtcaccc cctttcagtc aacgagattg aagaggcgcg gggcggcgcg cgaatcgacc
      901 ctttacgggc gcggcgcgcg gcggcacgtt gtttgaatgt gtccgggtag tcgctcgccc
      961 gcgccgcggg acctcacatc gctaataagg tgcgctcacc cgttcagttc cattgcttcc
     1021 tcgacacgga tttcgtgaga ggccgatttt gatgccgaag aaatgtatcc ccgttttact
     1081 gctcgccgcg tcactcttcg cccccctggc gggcgcctcg accgcgcccg ccccgaagac
     1141 cgtcctcgcc aacgccgtcc cgccgctccc gctcgccgcg tcgcgcatcg aatcggaact
     1201 cacgcactga cggcagcgcc gcgcggttgt tgacggcgcc cacgtgttga cgttataagc
     1261 acgtcgcgcc gcctcggtgc gccgcgactc atctttccgc gaaaggaacg ttgatgaaga
     1321 agagattgac agccacactt ttcaccctgc tgctggcgac gagcgccctc ggcctgcaag
     1381 cccccgcgtc atggacgaag ttcacgtcgc aggcgggccg cttctccgtc atgctgcccg
     1441 ccgagccgaa ggaggagaag gagacgaaga acgacggcgg cccgctctcg ccgtacacga
     1501 acaccctcta caccgcgcag ggcgccggcg ccgtctacct cgtcggctgg acggactaca
     1561 agccgggcgt gcggatcaac gtgccgggcg agatcaaagc caaccgcgac aacttcgtca
     1621 acgccatcaa agccacggtc gccacggaga aggcgatcaa gctcggcacg caccccggca
     1681 ccgagttcac cgccgagaac gcgcaggcgt ttttccacgt ccgcgtctac gtcatcggca
     1741 ggcgtcccta catcctcgtc gcgctcaggc ccaaaggcac acaagacccg aacg
//
LOCUS       DBNGOH010000196         3368 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1312671, whole genome shotgun sequence.
ACCESSION   DBNGOH010000196 DBNGOH010000000
VERSION     DBNGOH010000196.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3368)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3368)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3368
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1312671"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3114)
                     /locus_tag="AC159P_03750"
     CDS             complement(<1..3114)
                     /locus_tag="AC159P_03750"
                     /inference="COORDINATES: protein motif:HMM:NF051698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR3694599.1"
                     /translation="MRAFPRRRWLPTATRLALVCAFIVAGVATAFAQADATSANLSGY
                     VRDQQGAVVTGATVRARNAATSVERETTTNDEGFYQLTNLPPGAYEVSVEAPNFKKSV
                     LPDVTVTVGQRADLDISLEVGAISDVVTVTGASTELVETGKTNVSTTIDETRIDNLPI
                     NQRDYINFTLTTSTVTRDNGRPIGPAPTSGLNFGGQRGRSNLVQVDGADNTDNSVNAA
                     RSTVSQEAVQEFQVVNNSFAPEFGRTAGGVVNVVTKGGTNEVHGNVFGFIRHKSIQAK
                     NAFAPIIDGDPDKKPPFTRAQYGATIGGPLRRDRTFAFFAFEQRKRQESGFFTSDVIQ
                     GLTSSVTIGAPFLPFTQTFDRITSAQAGYVQTLLTTAGQLIATGVPANIAQGQALAGA
                     AVQYATLSSSGGQTGLNGRSTLISPGGAITAGTIIGQRFFLTGAPVPVSANAFRPLTQ
                     LARVFPIEEDTTFSSVRGDHRINDAHNLTMRFSYNPSDITGIQVESQNQALGQNDFSR
                     TGIQTLRDTAFVTTLNSTLSNTVVNEARFNFSSRRATFTSQNGGATAYNISGVAFIGR
                     ELFSPVIRTETRYQYTDNINWVWGGHTFKFGGDANFISVDAVFELNFAGLYNFGGLSA
                     TTLAAFPTVGPAAPPAFTPVQQYGLGFPGNYIQGFGNPVSNLKNRPFAVFAQDSWKIN
                     NRFTINYGVRYDYEQTQQLTVQDFRDPLSGLVVSAATTQQFYDAFGIQQGFPRDKNNI
                     APRVGVAWDLKGDGNTVLRAAAGIFYDHPLLAIGFNSDIADNVQQQQFIALPGSPAPD
                     QLLNAVQIFQGTVCTGQTGNPLCPPNFRTPGVAAGTDYQFGRQRFNDQTFPGFGPFLP
                     FSLPVSTDFEFAYANQANVALERKLTENMSFSASWLYVGARHLPHPTNLNAPNNTALI
                     ENFRRCTGALPTNTTAVLTLNPATCTNVVAAPIPGLVAVTTRGTVVQPFVANLFRPSG
                     PNYFFTQAVTGGAVTPAVLNSVLTATGALARPGFVTTFGDINAQVSDGNSVYHAGTFD
                     VKRRFANN"
BASE COUNT          521 a          964 c         1295 g          588 t
ORIGIN      
        1 gttgttggcg aagcggcgct tcacgtcgaa ggtgccggcg tggtagacgg agttgccgtc
       61 ggagacctgc gcgttgatgt cgccgaaggt agtgacgaag ccggggcggg cgagcgcgcc
      121 cgtcgccgtc agcacggagt tcaagaccgc gggcgtcacc gcgccgccgg tgacggcctg
      181 cgtgaagaag tagttcggcc ccgaggggcg gaacaggttg gcgacgaagg gctggacgac
      241 cgtgccgcgc gtggtgacgg cgacgaggcc ggggatcggc gcggcgacga cgttcgtgca
      301 ggtcgccggg ttgagcgtga gcacggcggt cgtgttggtc ggcagcgcgc ccgtgcagcg
      361 gcggaagttc tcgatgagcg ccgtgttgtt cggcgcgttc aggttcgtcg ggtgcggcag
      421 gtggcgcgcg ccgacgtaga gccagctcgc cgagaaggac atgttctcgg tgagcttgcg
      481 ttcgagcgcg acgttggcct ggttggcgta ggcgaactcg aagtcggtcg agacgggcaa
      541 agagaacggg aggaacgggc cgaagccggg gaaggtctgg tcgttgaagc gctggcgccc
      601 gaactgatag tcggtgccgg cggcgacgcc cggcgtgcgg aagttcggcg ggcacaaggg
      661 gttccccgtc tgcccggtgc agaccgtgcc ctggaaaatc tggacggcgt tcaagagctg
      721 gtccggcgcg gggctgccgg gcagcgcgat gaactgctgc tgctgcacgt tgtcggcgat
      781 gtcggagttg aacccgatgg ccaggagcgg gtggtcgtag aagatgccgg cggcagcgcg
      841 caagaccgtg ttgccgtcgc ccttcaaatc ccacgcgacg ccgacgcgcg gcgcgatgtt
      901 gttcttgtcg cgcgggaagc cctgctggat gccgaaggcg tcgtagaact gctgcgtggt
      961 ggcggcgctc acgacgaggc cggagagcgg gtcgcggaag tcctgcacgg tgagctgctg
     1021 cgtctgctcg tagtcgtagc gcacgccgta gttgatggtg aagcggttgt taatcttcca
     1081 cgagtcctgc gcgaagacgg cgaaggggcg attcttgagg ttgctgacgg ggttgccgaa
     1141 gccctgaatg tagttgccgg ggaagcccaa accgtactgc tggacgggcg tgaaggccgg
     1201 gggcgcggcg gggccgaccg tggggaacgc ggcgagcgtc gtggccgaga ggccgccgaa
     1261 gttgtagagg cccgcgaagt tcagctcgaa gacggcatcg acgctgatga agttggcgtc
     1321 gccgccgaac ttgaaggtgt ggccgcccca cacccagttg atgttgtctg tgtactggta
     1381 gcgcgtctcg gtgcggatga cgggcgagaa cagctcgcgc ccgatgaagg cgacgcccga
     1441 gatgttgtag gcggtcgcgc cgccgttctg cgaggtgaag gtcgcgcggc gcgagctgaa
     1501 gttgaagcgc gcctcgttga cgacggtgtt cgagagcgtc gagttgagcg tggtgacgaa
     1561 cgcggtgtcg cgcagcgtct gaatgccggt gcgcgaaaaa tcgttctggc cgagcgcctg
     1621 attctgcgat tcgacctgaa tgccggtgat gtccgaaggg ttgtagctga agcgcatcgt
     1681 caggttgtgc gcgtcgttga tgcggtggtc gccgcgcacg gaggagaagg tcgtgtcctc
     1741 ttcgatgggg aagacgcgcg cgagctgcgt cagggggcgg aaggcgttcg cgctgacggg
     1801 gacgggcgcg ccagtcaaaa agaagcgctg gccgatgatg gtgccggcgg tgatggcgcc
     1861 gccggggctg atgagcgtgc tcctgccgtt gaggccggtc tgcccgccgc tcgaagagag
     1921 cgtcgcgtac tggacggcgg cgcccgcgag cgcctgcccc tgcgcgatgt tggcgggcac
     1981 gcccgtcgcg atgagctggc cggcggtcgt cagcaaagtc tgcacgtagc cggcctgcgc
     2041 cgaggtgatg cggtcgaagg tctgcgtgaa ggggaggaac ggcgcgccga tggtgacgct
     2101 gctcgtcaac ccttgaatca cgtcgctcgt gaagaagccg gactcctgcc gcttgcgctg
     2161 ctcgaaggcg aagaaggcga aggtgcggtc gcggcgcaag gggccgccga tggtcgcgcc
     2221 gtactgcgcg cgcgtgaagg ggggcttctt gtcggggtcg ccgtcgatga tgggcgcgaa
     2281 ggcgttcttg gcctggatcg acttgtggcg gatgaagccg aagacgttgc cgtgcacctc
     2341 gttggtgccg cccttggtga cgacgttgac gacgccgccc gccgtgcgcc cgaactcagg
     2401 cgcgaacgag ttgttgacga cctgaaactc ctgcaccgcc tcctgcgaga ccgtgctgcg
     2461 cgcggcgttc accgagttgt ccgtgttgtc ggcgccgtcc acctggacga ggttcgagcg
     2521 cccgcgctgg ccgccgaagt tcaaaccgga ggtgggcgcc gggccgatgg gccgcccgtt
     2581 gtcgcgcgtg acggtcgagg tcgtcaaggt gaagttgatg tagtcgcgct ggttgatcgg
     2641 caggttgtcg atgcgcgtct cgtcgatggt ggtcgagacg ttggtcttac ccgtctcgac
     2701 cagctcggtg ctggcgcccg tgaccgtgac cacgtcggag atggcgccga cttcgagcga
     2761 gatgtcgagg tctgcgcgct ggccgaccgt gaccgtcacg tccggcagca cggatttctt
     2821 gaagttgggc gcctcgaccg agacctcgta ggcgcccggc ggcaggttcg tgagctggta
     2881 gaagccctcg tcgttggtgg tcgtctcgcg ctcgacgctc gtcgcggcgt tgcgcgccct
     2941 gacggtcgcg cccgtgacga ccgcgccctg ctgatcgcgc acgtagccgc tcaggttggc
     3001 ggaggtcgcg tcggcctgcg cgaacgcggt cgcgacgccg gccacgataa aggcgcacac
     3061 gagcgccagt ctggttgcgg tcgggagcca ccgccgtcgt gggaacgcac gcatatcttc
     3121 ttctccttag gaacgaaata ggggtctcgg caaacatcgg gcgagggcga tgcgcccgcc
     3181 tgcgcggact atggcggccc gcggttgact caaagttcgc acacaccttg cagcgaccgc
     3241 gcgacgggca agctataggt tctggtcacg cgcttgattt ttaggaggtc tggcggcgcc
     3301 tcgggaatcg gccttcacgc cggaacaaac tacggcactg gcgacaactt tgtaccttaa
     3361 aactcgtc
//
LOCUS       DBNGOH010000197         5188 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3511984, whole genome shotgun sequence.
ACCESSION   DBNGOH010000197 DBNGOH010000000
VERSION     DBNGOH010000197.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5188)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5188)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5188
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3511984"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..749
                     /locus_tag="AC159P_03755"
     CDS             <1..749
                     /locus_tag="AC159P_03755"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HIR3694600.1"
                     /translation="PHVVIADARLRGISAQDLGRVLNDAQETTPLILVGDGRADVARR
                     ASAMEAGASDYFEVPAELALVVARARQLVAHRQLVDKLRAEADRDYLTGLANRRRFRK
                     ALGQEVERWRRYKVPCALLLVDIDHLKKINDAHGHSTGDRAIRAVAEALAFHSRDNDT
                     AARLGGEEFALLLAGADCERAFAAAERLRLSVAAHPVGEAGLITVSLGVAACPAHAQS
                     ERTLYAASDRALYLAKHEGRNRTAIAVDHQ"
     gene            923..2965
                     /locus_tag="AC159P_03760"
     CDS             923..2965
                     /locus_tag="AC159P_03760"
                     /inference="COORDINATES: protein motif:HMM:NF012546.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HIR3694601.1"
                     /translation="MLTRGLYRRLLLVALLVQLTAPALVGVGARDHKGDSAAWSPELS
                     LKVKTVGPVRVSPDGRRVAYNVTAPVMTADRSELVAQIHVANTDGTDNFQLTFAEKTS
                     NNPQWSPDSRRLAFASTRNGKNNLYLLRLSGGEAEQLTDTKSAVGAFAWSPDGKWIAY
                     TMTDMPGEEEEKQTKGRDDWKWVEENVKLSRLYVLPVERDADGKRPARRLTAADYNIG
                     ELDWSPDGKSIVYSHSKSPKADYWTTLDVSVVDVQSGQSKTLAATPAAEYQPLFSPDG
                     KWVALSVTDNPPRWARSVTLHVVPVGGGAAKALPATFDGQPNLVGWSADGRQLYYNEA
                     RGTGSRLYALNVGTGAITELEKGADVFGAFDLNEGGTWFGFTSQSSERAPEAFVTRVG
                     DFKPVQVSRANADLALPPLGKTEVVRWKSTDGKEIEGLLTYPVGYAQGRRVPLLLNVH
                     GGPAGVFQQTFIGGRGSYPIATVAARGYAVLRPNPRGSSGYGVEFRRANIKDWGGMDY
                     QDLMTGVDHVVKMGVADPERLGVMGWSYGGYMTSWIITQTKRFKAASAGAPVTNLMSF
                     TGTADIPGFIPDYFGAQPWDDLDTYRKHSPMFNVRGVTTPTLIQHGDADERVPVSQGY
                     ELYNALRAQNVPVRMLVLPRQPHGPNEPRMILKVMQTNLEWFDKYLKNGTEESRGQ"
     gene            3199..4419
                     /gene="trpB"
                     /locus_tag="AC159P_03765"
     CDS             3199..4419
                     /gene="trpB"
                     /locus_tag="AC159P_03765"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008098333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HIR3694602.1"
                     /translation="MKDATQTLPDERGHWGPYGGRYVPETLMAPLEELAEAYAAARLE
                     EAFRAEYAALLRDFSGRPTPLFRAARLTHKAGGASIYLKREDLSHTGSHKINNALGQV
                     LLARRMGKGRVIAETGAGQHGVATATVCALFGLKCVVYMGEEDMRRQSLNVFRMRLLG
                     AEVRGVASGSRTLKDAINEALRDWVTNVSDTYYLLGSALGPHPYPRMVRDFQSVIGHE
                     ARAQILEREGRLPDALVACVGGGSNSIGLFYPFLEDEGVRMIGVEAGGTGAELGQHAA
                     RFNRAGGGRPGVLQGTLSYVLQDERGQIASTHSVSAGLDYPSIGPEHAYLHDEGRVEY
                     VSATDDEALEAFQALSRLEGIIPALESAHAVAHALKLAREMKREEIILLNLSGRGDKD
                     VQQVAEAGVGGGVA"
     gene            4416..>5188
                     /gene="trpA"
                     /locus_tag="AC159P_03770"
     CDS             4416..>5188
                     /gene="trpA"
                     /locus_tag="AC159P_03770"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HIR3694603.1"
                     /translation="MSRIAEAFETLRREGRRGFVPFITTGDPDVMTTRALVVELARAG
                     ATVVELGVPFSDPVADGPTIQRASERALREGAGIKEALYVVADARRETQVPVVLFSYF
                     NPLMQYGVERLARDASLAGIDGVLVTDLVPEEAADFNATLARHQLDQIFLAAPTTSDA
                     RLRLIAERASGFVYAVSRAGVTGARTELSDEAERLVARVRQHTDLPVAVGFGISTREQ
                     VHDTWRYADAAVVGSAIVSEIEKHAAAPDLVARVGAFART"
BASE COUNT          874 a         1831 c         1726 g          757 t
ORIGIN      
        1 gcccacacgt ggtcatcgcc gacgcgcgtc tgcggggcat cagcgcgcaa gacctcgggc
       61 gcgtgctgaa cgacgcgcag gagacgacgc cgctcatcct ggtcggcgac gggcgggcgg
      121 atgtcgcgcg gcgcgcgagc gcgatggagg cgggcgcgtc cgactacttc gaggtgccgg
      181 cggagctggc gctcgtcgtc gcgcgcgcgc ggcaactggt cgcgcacagg cagctcgtcg
      241 ataagctccg cgccgaggcc gaccgagatt atttgacggg cctcgccaac cgccgccgct
      301 tccgcaaggc gctcgggcag gaggtcgagc ggtggcggcg ttacaaggtg ccgtgcgcgc
      361 tgctgctcgt ggacatcgac cacctgaaga agatcaacga cgcgcacggc cactcgacgg
      421 gcgaccgcgc catccgcgcc gtcgccgagg cgctcgcctt ccactcgcgc gacaacgaca
      481 cggcggcgcg gctcggcggc gaagagttcg cgctgctgct cgcgggcgcc gactgcgaga
      541 gggctttcgc cgccgccgag cgcctgcgcc tgtcggtcgc ggcacatccg gtgggcgagg
      601 ccgggctgat caccgtcagt ctcggcgtcg ctgcgtgccc cgcgcacgcg cagtccgagc
      661 gcacgctcta cgccgcgagc gaccgcgccc tctacctcgc caagcatgag gggcgcaacc
      721 gcacggctat cgccgtcgat caccaataag agtcggtttc gaaacctcga tgctgatcac
      781 aactgtaggg gcagggcaag tacgcggccc gttaacgtcc gactgattca acgttaaagc
      841 taacctgagc gcgggccgcg gacaagccct gcccctacag ttgtgcctgt gacgcactct
      901 gaaggcggaa ggagagtcgc tgatgttgac gagaggattg tatcgaaggc tgttgttggt
      961 cgcgctgctc gtgcagttga cggcacccgc gctggtcggc gtcggggcgc gagaccacaa
     1021 gggcgattct gcggcgtggt cgccggagtt gtctctgaag gtgaagaccg tggggcctgt
     1081 gcgcgtctcg ccggacggca ggcgcgtggc ctacaacgtg accgcgcccg tgatgacggc
     1141 ggacaggagc gagctggtcg cgcagattca cgtggcgaac accgacggca cggacaactt
     1201 ccagttgacc ttcgcggaga agacctcgaa caacccgcag tggtcgcccg actcgcggcg
     1261 gctcgccttc gcctcgacgc gcaacggcaa gaacaacctc tacctgctgc gcctctcggg
     1321 cggcgaggcc gaacaactca ccgacacgaa gagcgccgtc ggcgccttcg cgtggtcgcc
     1381 cgacggcaag tggatcgcct acacgatgac cgacatgccg ggcgaggagg aggagaagca
     1441 gacgaagggg cgcgacgact ggaagtgggt cgaagagaac gtgaagctca gccgcctcta
     1501 cgtgctgccg gtcgaaaggg acgccgacgg caagcggccc gcgcgcaggc tgaccgccgc
     1561 cgactacaac atcggcgagc tggactggtc gcccgacggc aagtcgatcg tctactcgca
     1621 ctcgaagtca ccgaaggccg actactggac gacgctcgac gtgtcggtcg tcgatgttca
     1681 gagcggacag tcgaagacgc tggccgcgac ccccgccgcc gagtaccagc cgctcttctc
     1741 gcccgacggc aagtgggtcg ccttgagcgt cacggacaac ccgccgcgct gggcgcgtag
     1801 cgtgacgctg cacgtcgtgc cggtgggcgg cggcgcggcg aaggctctgc ccgccacctt
     1861 cgacgggcag ccgaacctcg tcggctggtc tgcggacggc aggcagctct actacaacga
     1921 ggcgcgcggc accggcagcc gcctctacgc gctcaacgtc ggcaccggcg ccatcacgga
     1981 gcttgagaag ggggcggacg ttttcggcgc cttcgacctc aacgagggcg gcacgtggtt
     2041 cggcttcacc tcgcagtctt cggagcgcgc gcccgaggcg ttcgtcacgc gcgtcggcga
     2101 cttcaagccc gtgcaggtca gccgcgccaa cgcggacttg gccttgccgc cgctcggcaa
     2161 gacagaggtc gtccgctgga agagcacgga cggcaaagag atcgaggggc tgctgaccta
     2221 cccggtcggc tacgcgcagg ggcggcgcgt gcctttgctg ctgaacgtcc acggcgggcc
     2281 ggcgggcgtc ttccagcaga ccttcatcgg cggtcgcggc tcgtacccga tcgcgacggt
     2341 cgccgcgcgc ggctatgcgg tcttgaggcc caacccgcgc ggctcgtccg gctacggcgt
     2401 cgagttccgc cgcgccaaca tcaaggactg gggcggcatg gactatcagg acttgatgac
     2461 cggcgtcgat cacgtcgtca agatgggcgt ggccgacccc gagcgtctgg gcgtgatggg
     2521 gtggagctac ggcggctaca tgacctcgtg gatcatcacg cagaccaagc gcttcaaggc
     2581 cgcctcggcg ggcgcgcccg tcaccaacct gatgagcttc acgggcaccg ccgacatccc
     2641 cggctttatc cccgactact tcggcgcgca gccgtgggac gatctggaca cctaccgcaa
     2701 gcactcgccc atgttcaacg tccgcggcgt caccacgccc acgctcatcc agcacggcga
     2761 cgccgacgaa cgcgttcccg tctcgcaagg gtacgaactc tacaacgcgc tgcgcgcaca
     2821 gaacgtcccc gtccgcatgc tcgtcctgcc gcgccagccg cacggcccca acgagccgcg
     2881 gatgatcctg aaggtcatgc agaccaacct cgaatggttc gacaagtacc tgaagaacgg
     2941 caccgaagag agccgcggcc agtgagcgcg cgttgttagg tctggactta tagcggtctg
     3001 cgaatgacca cgaccgtcgg cagaactgta ggggcagggc ttgtccgcgg cccgcgagtt
     3061 gcactggatt aacgttgagt gactccataa ctgcgggcag ggacaagccc tgcccctaca
     3121 gtcttcacgt acgggttcga gtccatccgc ggctgattat cactttgcgg tgcggcttgg
     3181 tatgattcgc gctgtcacat gaaagacgcg acacaaactc tccccgacga gcgcggccac
     3241 tgggggccgt acggcgggcg gtacgtgccc gagacgctga tggcgccgct cgaagagctg
     3301 gcagaggctt acgcggcggc gcgtctcgaa gaggcgtttc gggccgagta cgccgcgctc
     3361 ctgcgagact tctcgggccg cccgacgccg ctcttccgcg ccgcacggct gacccacaaa
     3421 gcgggcggtg cttccatcta tctcaagcgc gaagacctct cgcacacagg ctctcacaag
     3481 atcaacaacg cgctcgggca ggtcttgctc gcccgccgca tggggaaggg gcgtgtcatc
     3541 gcggagacgg gcgccggcca gcacggcgtc gccacggcca ccgtgtgcgc gctcttcggc
     3601 ctcaagtgcg tcgtctacat gggcgaggag gacatgcgac ggcagagcct caacgtcttc
     3661 cgcatgcgcc tgctcggcgc ggaggtgcgc ggcgtcgcgt ccggctcgcg cacactgaag
     3721 gacgccatca acgaggcgct gcgcgactgg gtgacgaacg tctcggacac ttactatctc
     3781 ttgggcagcg cgctcggccc gcacccgtac ccgcgtatgg tgcgcgactt ccagagcgtc
     3841 atcgggcacg aggcgcgcgc gcagatactc gaaagggaag ggcgcctgcc cgacgcgctc
     3901 gtcgcgtgcg tcgggggcgg ctcgaactcc atcgggctgt tctacccgtt ccttgaagac
     3961 gaaggcgtgc ggatgatcgg agtcgaagcg ggcggcaccg gcgccgaact cggccagcac
     4021 gcggcgcgct tcaaccgcgc gggcggcggg cggcccggcg tcctgcaagg cacgctcagc
     4081 tacgtcctgc aagacgaacg agggcagatc gcttcgacgc actcggtctc cgccggactc
     4141 gactaccctt ccatcggccc cgagcacgcc tacctccacg acgagggccg cgtcgaatac
     4201 gtctccgcga ccgacgacga ggcgctcgaa gcctttcaag ctctctcccg cctcgaaggc
     4261 atcatccccg cgctcgaatc ggcccacgcc gtcgcgcacg ccctgaagct cgcgcgcgag
     4321 atgaagcgcg aagagattat cctcctcaac ctttccgggc gcggcgacaa ggacgtgcaa
     4381 caggtcgccg aggcgggcgt cggcggaggc gtcgcttgag ccgcatcgcg gaagcgttcg
     4441 agacgctgcg acgcgaaggc cgccgcggct tcgtcccctt catcaccacg ggcgaccccg
     4501 acgtgatgac cacgcgtgcc ctcgtcgtcg aactcgcgcg cgcaggggcg accgtcgtcg
     4561 aactcggcgt ccccttcagc gaccccgtcg ccgacggccc cacgattcag cgcgcttccg
     4621 aacgcgccct ccgcgaaggc gcgggcatca aagaggccct ctacgtcgtc gccgacgcgc
     4681 gccgcgagac acaagtgccc gtcgtcctct tcagctactt caacccgctc atgcagtacg
     4741 gcgtcgaacg cctcgcgcgc gacgcgagcc tcgcgggcat tgacggcgtc ctcgtcacag
     4801 acctcgtgcc cgaagaggca gccgacttca acgcgacgct cgcgcgccac cagctcgacc
     4861 agatcttcct cgccgccccg accacttcgg acgcgcgtct gcgcctcatc gccgaacgcg
     4921 ccagcggctt cgtctacgcc gtctcgcgcg cgggcgtcac cggcgcgcgc accgaactca
     4981 gcgacgaggc cgaacgcctc gtcgcgcgcg tccgccaaca cactgacctg cccgtcgccg
     5041 tcggcttcgg catctccacg agagagcagg tgcacgacac ctggcgctac gcagacgccg
     5101 ccgtcgtcgg ctccgccatc gtctcggaaa tcgagaaaca cgccgccgcc cccgacctcg
     5161 tcgcccgcgt cggcgcgttc gcgcgcac
//
LOCUS       DBNGOH010000198         5206 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_579637, whole genome shotgun sequence.
ACCESSION   DBNGOH010000198 DBNGOH010000000
VERSION     DBNGOH010000198.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5206)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5206)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5206
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_579637"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1008
                     /gene="lpxK"
                     /locus_tag="AC159P_03775"
     CDS             <1..1008
                     /gene="lpxK"
                     /locus_tag="AC159P_03775"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - lipid-A 4'-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HIR3694604.1"
                     /translation="LRNRGYDRNPAKTTRVDVPVVSIGNLTLGGTGKTPLVEWVARAA
                     SEGGRRVCVLTRGYGREDVRVRVVVSDGERVLADAREGGDEPRLLAESLLGKAAVVSD
                     RDRVVAARWASEHLQTEVFVLDDGFQHLRLARELDIVTVDATAPWGGGHLLPRGRLRE
                     PLEGLARAGCVVITRADLAGDLDALRAEVGRLSGGAAAVITSRVKTLRLSPLAPSSPP
                     VELEGGAGGQTVAAFCAVGNPWAFFKHLRKDGYQLSHTRVFADHHVFTQRDAETVARE
                     AEQSGARLLLTTAKDAVKLRSLEFALPVYVVEIGVEFDEEETLRGLVREAIERPRRER
                     G"
     gene            complement(1227..2948)
                     /locus_tag="AC159P_03780"
     CDS             complement(1227..2948)
                     /locus_tag="AC159P_03780"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HIR3694605.1"
                     /translation="MKTCPRCGKQYEDANTLCPADGAVLKKDDTLIGNTLTDKYRIEE
                     RIAEGGMGAVYRATHILMEKTVAIKVLHPALAADDKIVARFTRDAKAASRISHPHALT
                     VTDFGEDKDGVVFLVMEYLRGRTLKEVIRQDGPMPLRRAVEIVRQVCGALEAAHAEGV
                     VHRDLKSDNIMLEEASGSDWAKVLDFGIAKIKENFGDEDPALTAPNLIIGTPQYMSPE
                     QCSQASEIDSRSDIYSLGVIIYEMLAGHVPFAGDSPTAIMMKHMQEAPPSLLEERPDL
                     PPEVGRIVAQALAKRPEDRFQKAGQLSDALAQAAAAVTSAASLKRPDTDRIIVPTAAN
                     EPPRTTDSSVFEEDEATVVSGEVIPLRDAHTGETPPPFVTGQPPPPPPAHSFNPWRIA
                     LPAIAALAVIFAVVYAFQQRGGTIGAGQPPLTTDPNSQPVQSITPPTGSAESNVAPAT
                     NTNGGGAPYDPNAGQIVNTQTLGNTNQSADAEATPTPADGGAANTNQTRQENSNTADE
                     PTPEATPAPANRNANRRAPANTNTSDDDDDDPPPPPPPAKKDPPAPAPTAAPAIKLES
                     NDPTAAP"
     gene            3270..4625
                     /locus_tag="AC159P_03785"
     CDS             3270..4625
                     /locus_tag="AC159P_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011985084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR3694606.1"
                     /translation="MGREHLVIVGGVAAGLAAAMEARRRAPELDITVLEKTGDISYGA
                     CGLPYVVSGLIPSLDRLVLHTPDYFRERHRIDVRLRSEALEVFPAKSVLRFVEDGAER
                     ETGYGALVIATGAAAVCPPLPGRDLEGVFVLRHLRDGRRMIEFIDRRRPLTAAVVGAG
                     YIGLEMAEAFAARGLRVTVVEASENVMGTLGGRARDRVADELREHGVEVVFGEKVVAF
                     EGTAGSVARVVTESGRAVEADVVSIGVGVRPEVGLARGAGLQLGESGAVSVDAGQRTS
                     AANVYAAGDCCEVLHRVSGRGVWHPLGQPAVRQGWVAGANAARSDSTVEERYAGVVGT
                     NAVKVFRLEVARTGLSLAEAREAGFDASEAEDETPSRAGYYPGGSKISTRVVSDRTGR
                     LLGAQMVAHEGVAQRIDVYAAALHANLKLEDLERLDLAYAPPFAPTIDPILRAVHKAK
                     T"
     gene            complement(4707..>5206)
                     /locus_tag="AC159P_03790"
     CDS             complement(4707..>5206)
                     /locus_tag="AC159P_03790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694607.1"
                     /translation="RRTDEEEDDSIFEPSRRGPARTRPRSSQAQTLPAPVEPGAGLGQ
                     RVTVTVPGNSQGQDTGLDGRAGDQLTITAEGTIVAGQRVGSVNANGRQGGFFGNPRYP
                     FPQAGAGALLGLVRDTAGRQSQPFLVGTQLTFTAQADGRLFLLVNDDNYNDNSGEFIV
                     RIRLN"
BASE COUNT          702 a         1613 c         1966 g          925 t
ORIGIN      
        1 ctgcgcaatc ggggctatga ccgaaatccg gccaagacga cccgcgtgga tgtgccggtg
       61 gtgagcattg gcaatctgac actcggcggg acgggcaaga cgccgctcgt cgagtgggtg
      121 gcgcgcgcgg cctctgaggg cgggcggcgc gtgtgcgtgt tgacgcgcgg ctacgggcgc
      181 gaggatgtgc gcgtgcgcgt ggtcgtgtcg gacggcgagc gcgtgctggc cgacgcgcgc
      241 gaggggggcg acgagccgcg gctgctggcg gagagcctgc tcggcaaggc ggctgtcgtc
      301 agcgacaggg atcgcgtcgt ggcggcgcgt tgggcgtcgg agcatttgca gacggaggtg
      361 ttcgtgctcg acgacgggtt tcaacacctg cggctcgcgc gcgagctgga catcgtgacg
      421 gtggacgcga cggccccttg gggcggcggg cacctgctgc cgcgcgggcg cttgcgagag
      481 ccgctggaag gactcgcgcg cgcgggctgc gtggtcatca cgcgcgcaga cctcgcaggt
      541 gatctcgacg cgctgcgcgc agaggtcggg cggctgagcg gcggcgccgc cgccgtcatc
      601 acttcgcgcg tgaagacctt gcgcctcagc ccgctcgcgc cgtcgtcccc gccggtcgaa
      661 ctcgaggggg gtgccggcgg gcaaacggtc gcggccttct gcgcggtggg caacccgtgg
      721 gccttcttca aacacttgcg gaaggacggt tatcagttga gccacacgcg cgtgttcgcg
      781 gatcaccacg tcttcacgca gagggacgcg gagacggtcg cgcgcgaggc cgagcagagc
      841 ggcgcgcgtc tgctcctgac gacggcgaaa gacgcggtga agctccgctc gctggagttc
      901 gcgctgcccg tctacgtcgt cgagatcggc gtggagttcg acgaagagga gacgctgcgc
      961 ggcctcgtgc gtgaagcgat tgaacgacca cgaagggaac gcgggtagag cgtctccttc
     1021 atcgatgacc gtcaatagta actttcaaat tatattaaga cgttcggcaa tattgtaggg
     1081 gcagggcaag tccgcggccc gctacgtccg attgatccaa cgtcgcgcgt gacgtgcctg
     1141 cgggcaggga caagccctgc ccctacagtt atcgtgcgcg ttttacggtc tactgaaatt
     1201 tgctctggag gcgtgcgaca aaggattcag ggcgcggcgg tcgggtcgtt cgattcgagc
     1261 ttgatggccg gggccgcggt gggtgcgggc gcaggcgggt ctttcttcgc gggcggcggc
     1321 gggggcgggg ggtcgtcgtc gtcgtcgtcc gacgtgttcg tgttggcggg cgcgcgacgg
     1381 ttggcgttgc ggttcgcagg ggccggcgtg gcttcgggcg tcggctcgtc cgcggtgttg
     1441 ctgttctcct gacgggtctg gttcgtgttc gcggcgccgc cgtcggcggg ggtcggcgtg
     1501 gcttcggcgt ccgcgctctg gttcgtgtta ccgagggtct gcgtgttgac gatctggccc
     1561 gcgttcgggt cgtagggcgc cccgccgccg ttggtgttgg tggcgggcgc gacgttggac
     1621 tcggcggagc cggtcggcgg cgtgattgat tggacgggct ggctgttggg gtccgtggtg
     1681 agcggcggct gccctgcgcc gatcgtgccg ccgcgctgtt ggaaggcgta gacgacggcg
     1741 aagatgactg cgagcgccgc aatcgcgggg agcgcgatgc gccaggggtt gaagctgtgc
     1801 gcgggcggcg gcggcggggg ctgcccggtg acgaaaggcg gcggcgtttc gccggtgtgc
     1861 gcgtcgcgca gcgggatgac ctcgccgctg acgaccgtcg cctcgtcctc ctcgaagacg
     1921 ctgctgtcgg tggtgcgcgg cggctcgttg gccgcggtag ggacgatgat gcgatccgtg
     1981 tccggcctct tcaacgaagc cgcggaggtg acggcggcgg cggcctgggc gagcgcgtcg
     2041 gagagttggc cggccttctg gaagcggtct tcggggcgct tggcgagcgc ctgcgcgacg
     2101 atgcgcccga cctcgggtgg caggtctggc cgctcttcga ggagcgaggg cggcgcctcc
     2161 tgcatgtgct tcatcatgat cgcggtcggc gagtcgcccg cgaaggggac gtggccggcg
     2221 agcatctcgt agatgatgac gcccaggctg tagatgtcgg agcgcgagtc gatctcggaa
     2281 gcctgcgagc actgctcggg cgacatgtac tgcggcgtgc cgatgatgag gttcggcgcc
     2341 gtgagcgcgg ggtcttcgtc tccgaaattc tccttgatct tggcgatgcc gaagtcgagc
     2401 accttcgccc agtcgctgcc cgaagcctct tcgagcatga tgttgtcgct cttgaggtcg
     2461 cggtggacga cgccctcggc gtgcgcggct tcgagcgcgc cgcacacctg ccgcacgatc
     2521 tcgaccgcgc gccgcagcgg catcggcccg tcctgccgga taacctcttt gagcgtgcgc
     2581 ccgcgcaggt actccatgac gaggaagacg acgccgtcct tgtcctcgcc gaagtcggtg
     2641 acggtcaaag cgtgcgggtg cgagatgcgc gaggcggctt tggcgtcgcg cgtgaagcgg
     2701 gcgacgatct tgtcgtcggc ggcgagcgcc gggtggagca ccttgatggc gacggtcttc
     2761 tccatcagga tgtgcgtggc gcggtagacg gcgcccatcc cgccctcggc aatgcgctct
     2821 tcgatgcggt acttgtcggt gagggtgttg ccgatcagcg tgtcatcctt cttcaggacg
     2881 gcgccgtccg cggggcaaag cgtgttcgcg tcctcgtact gcttcccgca tctcggacag
     2941 gttttcatat cgacagactc acgcctcgcc ttccgttact ctcgattggc tggggtgatt
     3001 cgaagcgtat cacaccggtt cgccacgcgc aataatccca ctcaggtaaa cgatgaaagg
     3061 actttggatt ctagattttg gcgggcggat cgatgattga tgaccgctga ttgacggaag
     3121 cggcgctgtc atcaatcgtc aatcgtaaat ccgacatcaa gccaggatca tttcatcgcc
     3181 ctcttcccgt tttgccttcg gcgcgcgcgt cgagtaacct tgcgcgcgac gtttcttcga
     3241 ctacgaacgg cttgaggcgg cgggagagct tgggcagaga acaccttgtc atcgtcggcg
     3301 gcgtcgccgc gggcctcgcg gcggcgatgg aggcgcggcg ccgcgcgccc gaactcgaca
     3361 tcacggtgct tgagaagacc ggcgacatct cttacggcgc gtgcgggctg ccttacgtcg
     3421 tctccggcct gatcccttcg ctcgaccggc tcgtcctgca cacgcccgat tacttccgcg
     3481 agcgccaccg gatagatgtt cgcctgcgca gcgaggcgct cgaagtcttc cccgcgaaat
     3541 cggttctgcg ttttgtcgaa gacggggccg agcgcgagac gggttacggc gcgctcgtca
     3601 tcgccacggg cgccgccgcc gtctgcccgc cgctgccggg ccgcgacctc gaaggcgtct
     3661 tcgtgctgcg gcacctgcgc gacgggcggc gaatgatcga gttcatcgac cgccgccgtc
     3721 cgctgacggc ggcggtcgtc ggcgccggct acatcgggct tgagatggcc gaggctttcg
     3781 ccgcgcgcgg cttgcgcgtg acggtcgtcg aggcttccga gaacgtgatg ggtacgctgg
     3841 gggggcgcgc gcgcgaccgc gtggcggacg agctgcggga gcacggcgtc gaggtcgtct
     3901 tcggcgaaaa ggtcgtcgcg ttcgagggca cggcggggag cgtcgcgcgc gtcgtgacgg
     3961 agtccgggcg cgcggtcgag gcggacgtgg tttccatcgg tgtgggcgtg cgcccggagg
     4021 tcgggctggc gcgcggcgcg ggcttgcagc tcggcgagag cggcgccgtc tccgtggacg
     4081 cggggcagcg gacgagcgcg gcgaacgtct acgcggcggg cgactgctgc gaggtgctgc
     4141 accgcgtttc ggggcggggc gtgtggcacc cgctcggtca gcccgcggtg cggcagggct
     4201 gggtcgcggg cgcgaacgct gcgcgcagcg actctactgt cgaagagcgt tacgcgggcg
     4261 tggtcggcac gaacgcggtc aaggtcttcc gtctggaagt cgcacgcacc ggcctctcgc
     4321 tggcggaagc gcgcgaggcg ggcttcgacg cctccgaagc ggaagacgag acgccctcgc
     4381 gtgcgggcta ctatccgggc ggctcgaaga tttcgacgcg cgtcgtctcg gatcgcacgg
     4441 gccgcctgct cggcgcgcag atggtcgcgc acgaaggcgt cgcacagcgc atcgacgtct
     4501 acgccgccgc gcttcacgcg aacctcaaac tcgaagacct cgaacgcctc gacctcgcct
     4561 acgccccgcc cttcgcgccg accatcgacc ccatactacg cgccgtgcac aaggcgaaaa
     4621 cgtagaggtc ggtggtcggt ggtcagtgaa aacagaactc attctttcac tgatctccga
     4681 cctccgacct ctgacctctg ctcctctcag ttgagtcgta tccgcacgat gaactcgccg
     4741 ctgttgtcgt tgtagttgtc gtcgttgacg agcaggaaga gtctgccgtc ggcctgcgcg
     4801 gtgaaggtga gctgcgtgcc gacgaggaag ggctgcgact ggcggcccgc ggtgtcgcgg
     4861 acgaggccga gcagcgcgcc cgcgcccgcc tgcgggaacg ggtagcgcgg gttgccgaag
     4921 aaaccgccct gccgaccgtt ggcgttgacc gagccgacgc gctggcccgc gacgatggtt
     4981 ccttcggcgg tgatcgtcaa ctggtcgccc gcgcgaccgt cgaggccggt gtcctggccc
     5041 tgcgagttgc cgggcacggt gacggtgacc cgctggccga ggcccgcgcc cggctcaacg
     5101 ggagcaggga gggtttgggc ctgtgacgag cggggccgcg ttcgcgcggg gccgcggcgg
     5161 ctcggctcga agatgctgtc gtcctcctct tcgtcggtgc ggcgcc
//
LOCUS       DBNGOH010000199         2948 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4795014, whole genome shotgun sequence.
ACCESSION   DBNGOH010000199 DBNGOH010000000
VERSION     DBNGOH010000199.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2948)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2948)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2948
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4795014"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(107..898)
                     /locus_tag="AC159P_03795"
     CDS             complement(107..898)
                     /locus_tag="AC159P_03795"
                     /inference="COORDINATES: protein motif:HMM:NF024881.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor family protein"
                     /protein_id="HIR3694608.1"
                     /translation="MLTCEESQRAFSPYLDGELTRAEGAALAEHLDLCPVCRSEMERT
                     REMIRGLSLVARPAPPADLAASINVALMGERAARKASPTLPFGERVARWLEPHLMPYA
                     VGAFYSVLLFVAVFGALRNQMETLRNMAYAERLHAEVPVRVTWVNGVYGVTRTYTPGS
                     RSPFSGESPTLNPSGALAALATSPSSGDLPDDDDMIVVADVYGNGSASLAAVVEPPRN
                     RRVLTDLEDAFRKNPAFVPAALDQRPQTMRVVFVLQKMNVPDSSY"
     gene            complement(1367..2041)
                     /locus_tag="AC159P_03800"
     CDS             complement(1367..2041)
                     /locus_tag="AC159P_03800"
                     /inference="COORDINATES: protein motif:HMM:NF019265.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR3694609.1"
                     /translation="MSFGEPIALRAQAELERAADAAASAEDRAGPQSAEAQFIDRLRA
                     GDAAAFNRLVADRTGDIYALLCRLTDDAEEARDLTQETFLQAFRNIAGFRGDADLRTW
                     LYRIAVNQARNRWRWWKRRRRERTVSLDAPLAADSGTTLGAVLADADGEDPEQATLAR
                     ERERALLSALHTLSRPYREVIVLRDIEGFSYEEVAHSLSISVGTVKSRLSRGRTELRR
                     RLESSL"
     gene            2489..>2948
                     /locus_tag="AC159P_03805"
     CDS             2489..>2948
                     /locus_tag="AC159P_03805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694610.1"
                     /translation="MARTTKKRAKQLKHDKFRDTTMGLIDRAGDRLEGQGRTILYALA
                     GLVAALILFFVYQWWSDRRADGARLALGKAIQTAAAPVVTGTPIPGQTGPTFPSERER
                     AQKAVEEFQKVQQQYGAPYNELARYFAATNLLVVEREKGLAELEQLSKSGN"
BASE COUNT          504 a          975 c          997 g          472 t
ORIGIN      
        1 gagaagaaac agcggattcg gaattccgaa tccgctgttt cttctctgcg gcctctgcgc
       61 ctctgcgttc tccgcgttga agtgagcgcg cgctacattc aacgtcttag tagctgctgt
      121 cggggacgtt catcttctgg aggacgaaga cgacgcgcat cgtctgcggg cgctggtcga
      181 gcgcggcggg gacgaaggcc gggttcttgc ggaaggcgtc ttcgaggtct gtcagcacgc
      241 gacggttacg cggcggctcg accaccgcgg cgagcgaggc gctgccgttg ccgtacacgt
      301 ccgccacgac gatcatgtcg tcgtcgtcgg gcaaatcgcc gctcgacggc gacgtggcga
      361 gcgcggccag cgcgcccgac gggttgagcg tcggcgattc gccgctgaac ggcgagcgcg
      421 accccggcgt gtaggttcgc gtcacgccgt agacgccgtt gacccacgtg accctgacgg
      481 gcacctccgc gtggagccgt tcggcgtagg ccatgttgcg cagcgtctcc atctggttgc
      541 ggagcgcgcc gaagaccgcg acgaagagca gcacggagta gaacgccccg accgcgtagg
      601 gcatcaggtg cggttcgagc cagcgcgcga cgcgctcgcc gaaggggagc gtggggcttg
      661 ccttgcgcgc ggcgcgttcg cccatgaggg cgacgttgat cgaggcggcg aggtctgcgg
      721 gcggcgcggg ccgcgcgacc agagacaggc cgcggatcat ctcgcgcgtc cgctccatct
      781 ccgagcggca gacggggcac aggtcgaggt gttcggccaa cgccgcgccc tcggcgcgcg
      841 tcagctcacc gtcgaggtac ggcgagaacg ctctctggga ttcctcgcaa gtcaacataa
      901 ttcgggggtc gggtgtcggg ggtcgggtgc cggggaagac cagttctgcg ctgacccccg
      961 acacccggcc cccggccccg gcttttgacg gatggctggc cgaccccggc tgctcccttt
     1021 taaaaaggaa gcagtttcgg gcgcccttcc ggtcggggct tgaagaccgc cgaagaccgg
     1081 tgcgcgtgcg gggacaagaa aaacgggtgt tgctggccgc gttttcaaaa tccgcccgcg
     1141 tccctgtccg gggcctcgcg cccgaaccga agagccgcct ctcgccgctt cctgacccac
     1201 ctggcaccgg gttcttttgg ttgaagccgg ggcagggcag ccatccgcca cgaaagattt
     1261 cggattgggg attgcggatt gaaggaaccg ggatgattga tgacagcgtc gccttcatca
     1321 atcgtcaacc tttcttcaat ccgcaatccc catccgcatt ccgcaattac aaagagcttt
     1381 ccaaacgtcg ccgcagttcg gtgcggcctc tcgataatct cgacttgacg gtgccgacgc
     1441 tgatcgagag cgagtgcgcg acctcctcgt agctgaagcc ctcgatgtcg cggagcacga
     1501 tcacttcgcg gtagggccgc gagagcgtgt gcagcgccga gagcagcgcg cgttcgcgct
     1561 cgcgcgcgag cgtggcctgc tcggggtctt cgccgtcggc gtccgcgagc acggcgccga
     1621 gcgtcgtccc ggagtcggcg gcgagcggcg cgtccagcga gacggtgcgc tcgcgccggc
     1681 ggcgcttcca ccagcgccag cggttgcgcg cctggttgac ggcgatgcgg tagagccacg
     1741 tccgcaagtc cgcgtcgccg cgaaaaccgg cgatgttgcg gaacgcctgg aggaaagtct
     1801 cctgcgtcag gtcgcgcgcc tcttcggcgt cgtcggtcag gcggcagagc agcgcgtaga
     1861 tgtcgcccgt gcggtcggcc accaggcggt tgaaagccgc cgcatcgccc gcccgcagtc
     1921 tgtcaatgaa ctgtgcttcg gcggactgcg gcccggcgcg gtcttccgcc gaggccgccg
     1981 cgtcggcagc ccgctccaac tccgcttgcg cgcggagggc gattggctct ccgaagctca
     2041 cagtcgtcct ccggcggacg tgacggggga gccgtaccgc ctcctgtctc ccctcgacag
     2101 accacataga caccgcccgc gccggccttg ttccaaaaaa tattccatcc cgaagataag
     2161 atgccgcgtc caccggattg gctcgcgcgg ggtgaccgtg gacaagatgt aaaaaagccg
     2221 agacggcgac gacttccagt tcataccaca aaaacgggcg gacggggaga aaagcagtag
     2281 gagagacggg gagaaaggga gtgggggaga cggggagaga agcggcgctg ttaattgtct
     2341 ccccatctcc ctccctccct ttctcccctt ctcgggtctc ccccgtccat ttgacaacgc
     2401 ctcgcggcgg tcgttagagt gaagcctttg cctcaagctc ccgaaagaga acgcactcta
     2461 ctgtgcgccc ttgcgacagg aaacaacagt ggctcgaacg acgaaaaaac gcgcaaagca
     2521 actcaagcac gataaattcc gcgacacgac gatgggcctg atcgaccgcg cgggcgaccg
     2581 cctcgagggg cagggccgca cgatcctcta cgcgctcgct gggctggtcg ccgcgctcat
     2641 cctcttcttc gtctaccagt ggtggagcga ccggcgcgcg gacggcgcac gcctcgcgct
     2701 cggcaaggcc atccagaccg ccgcggcgcc cgtcgtgacg gggacgccca tacccggcca
     2761 gaccggcccg accttcccca gcgagcgcga gcgcgcgcag aaggccgtcg aggagttcca
     2821 gaaggtgcag cagcagtacg gcgcgccgta caacgagctg gcgcgctact tcgccgccac
     2881 gaacctgctc gtcgtcgagc gcgagaaggg gctggccgag ctggaacagc tcagcaagtc
     2941 cggcaacg
//
LOCUS       DBNGOH010000200        10657 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3879940, whole genome shotgun sequence.
ACCESSION   DBNGOH010000200 DBNGOH010000000
VERSION     DBNGOH010000200.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 10657)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10657)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10657
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3879940"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1091)
                     /locus_tag="AC159P_03810"
     CDS             complement(<1..1091)
                     /locus_tag="AC159P_03810"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019238127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 protein"
                     /protein_id="HIR3694611.1"
                     /translation="MPIKRLRKGSRAAALALCLVVVTLPVSAQTQKPDGRSPYLDPSL
                     PLDRRVADLVSRMALEEKVSQMMNAAPAIPRLGVPEYDWWNEALHGVAFNGTATVFPQ
                     AIGLGATFNPRLVGRVAEVISTEARAKYHEAQRRGDRARFRGLTFWSPNINIFRDPRW
                     GRGQETYGEDPYLSGRLGVEFVKGLQGDDPKYLKVVATPKHYAVHSGPEPERHRIDAA
                     ATERDLRETYLPAFRATVTEGQAVSVMCAYNRINGEPACASTHLLGDILRGEWGFQGY
                     VVSDCGAIDDIFRRHNFTKTPEEASAVAVKQGQISEGEIDTAVKRLFEARFRLGMFDP
                     PELVKYAQIPFSENDSPAHRQLALEAARES"
     gene            complement(1268..2842)
                     /locus_tag="AC159P_03815"
     CDS             complement(1268..2842)
                     /locus_tag="AC159P_03815"
                     /EC_number="3.2.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0031222 - arabinan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HIR3694612.1"
                     /translation="MRALVFCLALMVAGLAAPSAAAQKVELSIDASKAGAKIDRNIFG
                     QFAEHLGKGVYEGVWVGPDSPIPNTRGIRNDVVAALRAIKVPNVRWPGGCFADEYHWR
                     KGIGPRRPETLNPNWGGVIEPNTFGTHEFMDFLEQVGAEAFLSVNVGSGTPQEAAEWL
                     EYLTAAQPTELAKERTANGRPAPYKIAYLGIGNESWDCGGNMTPDYYVSQLKIYSRFV
                     RNFNPVQQDKQQMLKIAVGPGGSEARFTEWTEAVMKAYQNRQWSWDMHGLSLHNYTVV
                     NWEKKFASVGFGEAEYAQILKNTLEMEGHISKHSAIMDKYDPQKRVALVVDEWGAWYA
                     PLPGSNPGFLYQQNSLRDAVLAALNLNIFARHSDRVRIANIAQMVNVLQAMILTDKEK
                     MVLTPTYHVFKLYVPFQDSTFVPVIFNAGTYTHGDVTLPRVDAVAAKGADGRLWLAVT
                     NVDPNRPAEIEASLSGVTATAATGETLTAPKVDSVNTFEVPNTVAPQPVSAKVQGGKL
                     TLRLEPKSVTVISVGQ"
     gene            complement(3455..7267)
                     /locus_tag="AC159P_03820"
     CDS             complement(3455..7267)
                     /locus_tag="AC159P_03820"
                     /inference="COORDINATES: protein motif:HMM:NF051698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR3694613.1"
                     /translation="MKRMLRACLTLCLFAAGAAAQSNTGRLVGTVSGPDGVIPGANVT
                     VTDMQTGRERTVVSTEDGSFTVPQLDAGTYTVTVTAPGFKTFTASAVKIDVGRDYSLT
                     AALEVGYISETVEVVAGTDVLNASNADLSTTVSPRQIQDLPLNGRNPLGLITLQAGTS
                     NNGAQATSINGQRPSATNITLDGLNIQDNFIRQSASASTQVALSSDDVAEFTVTTQNA
                     GGEQGLGASQVQLATPRGQNEFHGAVFEYNRNSRFAANTFFNNAAGNFGPNDPLVVAG
                     QATAGAARSPRTFRNFNQFGGRLSGRIIKNKLFFFGHYEGTRDRLSQPSLRTVLTPSA
                     RQGLFTYVDNGGVTRQVNLFALPITANNPPTGINPVIQSRFLANIPAGNSIEAGDQRN
                     TTGYRFNQKSDSDADNMTIRLDYDINSTNTLNAIYKRQTATGFVPDFSDLNGFDPTPP
                     SGFSTPNDSYVLAFRSTLTPVFTNEVRAGYVRSAQLFPELAGRQPNQFSQTNIAAGAL
                     AGFLSNPESTFLFQGRHTKTYTLQDNADYLWGDHGLRFGVQANAYRALRLNDGGVLPT
                     YVIGVGTNTPQITAAMFTNSALFPGGINTTDRTTANQLYAFLGGIVSQQSQTFNVLSA
                     DQGLVPLAGFRQDYAYENYNIYFSDQWRVRPNLTVNLGLRYELWPAVRERNGVLAEVI
                     VPEGKTVAEALLDPNGRLGTAGENVGGGKLFNTDKNNFAPSFSVAYSPQFRNKFLRGL
                     IPGDGRTVIRGGYRLSYFNDEFLKGSSGQGDQNSGKRENASRNNLNERLGALGTITTP
                     TVLIPRTFAQNNARAGGAIQSVATTDPNIKLPINHEYTVGIQREIGFQTAFEIRYVGA
                     FSNNVTRYTDVNPPELITNGFLDDFFRARSNQALTGQLNCTTAQNPGCQPLQVFINLG
                     TLSGTPGGIGNATTAPNATVANLVSTGLPGQLAATYVGNGLSGTVPLVANPNIGLGLL
                     LSNSARYNYNSLQMEVRRRFAQGLYFQGNYTFQKTLSNAPGVDQRRFEFQVYPKRDEL
                     EYSRAQTDTAHVFNFNSIYELPFGKGRHFFTDAGPWFDRLVGGWQLNTIVSLTTGAPF
                     SIVDPRGTFGSNARSARNTANSSLTKDQIQDLVGVFRTPCGVFLINPAVTTINQEALA
                     AGRCADIGASRGANGLGTAAFPGQIFFNVPPGEFGQLERFFLNGPMYFNIDASLFKNI
                     RITERVRLQLRAEAFNLFNRAHFAISAAQQLQSINANTFGRITQTGDPRLGDPQARVV
                     QFAARIEF"
     gene            7836..8876
                     /locus_tag="AC159P_03825"
     CDS             7836..8876
                     /locus_tag="AC159P_03825"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family RNA-guided transposase"
                     /protein_id="HIR3694614.1"
                     /translation="MTIHCGVDFHARQQTVCYCDSANGEVHLKELDHERDDVRGFYSA
                     LTGKVIIGIEASGYSTWFVELVEGLGHEVLIGDAAEIRRLAKRRQKNDRRDAGLILDL
                     LLKREFPQVHRPSFESREVLRLLRYRHKLVQVRTRAKNSLQAVAFSAGSVGRSKLLSK
                     AGRERFVLLPMSEAMIRQRDGWLSLVDDLSARIKSLDAWLGQQAKQDERVLRLQTHPG
                     IGLLTSLALVHGLEPVTRFDGGRKVAAYVGLDPMEYSSGDKQRFGSISKGGSRLLRYL
                     LVEAAQITVRRDEGLKRFYLRLLHRRGAQKAQVAVARKLLIRGYILLRDGIDYAEFLR
                     RGVEARPARLAP"
     gene            9068..10129
                     /locus_tag="AC159P_03830"
     CDS             9068..10129
                     /locus_tag="AC159P_03830"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family RNA-guided transposase"
                     /protein_id="HIR3694615.1"
                     /translation="MRAHNISLPDDETVFAGIDMHQRQWRVTIRTGEVELTSASIPGD
                     WQSLRRLLERYHGHPVEVVYEAGCFGYWLHDRVVEYGAQCIVTPPSLIPRESGNRVKT
                     DKRDSRKLALLLAKGMLKRVYVPTPQEREHRAVARRRRQLIRERVRAQNRIKAELRFY
                     GLRLPEITGRWSKLYVENLRRIRFSTRWQQESFESLLEQYEFFTQQIERQTRLLRSLS
                     QTAEYRERVKILQSIPGVGLIAAMEMLLELQDVARFRRADQLAAYVGLTPAQRSSGDV
                     VRMGRITRSGKNSLRGTLVEAAWRMVSRDSAVRQQYEQLKARAGAKRAIVAVARRLLL
                     RARRMLLDKRAYVAELAAA"
BASE COUNT         1760 a         3161 c         3771 g         1965 t
ORIGIN      
        1 gactcgcggg cagcttcaag tgcgagttgc cggtgcgcgg gcgagtcgtt ctcggagaaa
       61 ggtatctgcg cgtacttcac caactcgggc gggtcgaaca tccccagacg gaagcgcgcc
      121 tcgaacagac gtttgacggc cgtgtctatc tccccttcgg aaatctgccc ctgcttcacg
      181 gccacggccg aagcctcctc gggcgtcttc gtgaagttgt ggcggcggaa gatgtcgtcg
      241 atggcgccgc agtcggagac gacgtagccc tggaagcccc actcgccccg cagtatgtct
      301 ccgagcaggt gtgtgctggc gcaggcaggc tcgccgttga tgcggttgta ggcgcacata
      361 acagaaaccg cctggccttc cgtgacagtg gcgcggaagg ccggcaggta ggtctcgcgc
      421 aggtctcgct cggtcgcggc ggcgtcgatg cggtggcgct cgggctccgg gccggagtgg
      481 acggcgtagt gcttcggcgt cgcgacgacc ttcaggtact tcgggtcgtc gccctgcaag
      541 cccttgacga actcgacgcc gagacggccc gaaagatacg ggtcttcgcc gtaggtctcc
      601 tgaccgcggc cccagcgcgg gtcgcggaag atgttgatgt tcggcgacca gaaggtcagg
      661 ccgcggaagc gcgcgcggtc gccgcgccgc tgcgcctcgt ggtacttggc gcgcgcctcg
      721 gtcgagatga cttcggcgac gcggccgaca agccgcgggt taaatgtcgc gcccaagcct
      781 atggcctgcg ggaagacggt cgccgtgccg ttgaaggcga cgccgtggag cgcctcgttc
      841 caccagtcgt actcggggac gccgaggcgc gggatggcgg gcgcggcgtt catcatctgc
      901 gagaccttct cttcgagcgc catccgcgag acgaggtctg cgacgcgccg gtctaagggt
      961 aggctcgggt cgaggtacgg cgagcgtccg tccggcttct gcgtctgcgc ggagacgggc
     1021 aaagtgacga cgacgagaca gagcgcgagc gcggcggctc gcgagccttt gcggagacgt
     1081 ttgataggca tgtcgttgac cattcattgg gccgcgggca cccgaggcgg agctgtccgg
     1141 ccggtaacat cggccccacg agccgaatga accgccgggg gcacttcggt ctgaacttgt
     1201 gccgtaaccc gggtgcagag ggcggcggtc accgccagga cgattacgca gaagagtctc
     1261 atgggtgtca ctgcccgacc gagatgacgg tcaccgactt gggctccaat cgcagagtca
     1321 gcttaccgcc ctgaaccttc gccgagacgg gctgcggcgc gaccgtgttc ggcacctcga
     1381 aggtgttgac gctgtcaacc ttgggcgcgg tcaacgtctc cccggtggcc gcggtggccg
     1441 tgacgccgga caggctcgcc tcgatttcgg ccggccggtt ggggtcgaca ttcgtgaccg
     1501 ccagccacag cctgccgtcc gcgcccttcg ccgcgacggc gtccacgcgc ggcagcgtca
     1561 cgtcgccgtg ggtgtaggtt ccggcgttga agatgaccgg cacgaacgtg gagtcctgga
     1621 acggtacgta caacttgaag acgtggtagg tcggggtcag aaccatcttc tctttgtccg
     1681 tcaggatcat cgcctgaagg acgttgacca tctgcgcgat gttggctatg cgcacgcggt
     1741 ctgagtggcg tgcgaagatg ttgaggttga gagcggccag cacggcgtcg cgcagactgt
     1801 tctgctggta caggaagccg gggttgctgc cgggcagcgg cgcgtaccac gcgccccact
     1861 cgtccacgac gagagcgacc ctcttctgcg ggtcgtactt gtccatgatg gccgagtgct
     1921 tactgatgtg gccctccatc tccagagtgt tcttgaggat ttgtgcgtac tcggcctcgc
     1981 cgaacccgac ggacgcgaac ttcttctccc agttcacgac cgtgtagttg tgcagcgaga
     2041 ggccatgcat gtcccaactc cactggcggt tctgatacgc cttcatgacg gcctcagtcc
     2101 attcggtgaa acgcgcctcg gagccgcccg gcccgactgc gatcttgagc atctgctgct
     2161 tgtcttgctg caccgggttg aagttgcgga cgaagcggct gtagatcttg agctggctca
     2221 cgtagtagtc gggcgtcatg ttgccgccgc agtcccagct ctcgttgccg atgccgaggt
     2281 atgcaatctt gtagggcgcg gggcggccgt tggccgtgcg ctcctttgcc agttccgtcg
     2341 gctgcgcggc cgtcaggtac tccagccact cggctgcctc ctgcggggtg ccggagccga
     2401 cgttgaccga gaggaacgcc tcggcgccga cctgttcgag gaagtccatg aactcgtgcg
     2461 tcccgaaagt attcggctcg atcacgccgc cccagttggg gttgagcgtc tcgggccgcc
     2521 gggggccgat gcctttgcgc cagtggtatt catccgcgaa gcagccgccc ggccaacgga
     2581 cgttcggaac ctttatcgcc ctgagcgccg cgacgacgtc gttgcggatg ccgcgcgtgt
     2641 tcgggatggg cgagtccggc ccgacccaga cgccctcgta aacgcctttg ccgaggtgct
     2701 cggcgaactg gccgaagatg ttgcggtcga tcttcgcccc ggcctttgag gcatcgatag
     2761 acaattcgac cttctgcgct gccgcggagg gagcggccag tcccgcaacc atcagcgcga
     2821 gacagaaaac cagtgcgcgc atgacgagcg aatccctccg gcccgaacca agagtggaag
     2881 ggcgaagcga gtggagaaaa acgtgcgccg cgccgacgag actctgcccg ccggaaggcc
     2941 ggcgtgtcag gcaatgaggg tcgtttatga tttctgaagc cggtgtcgag atcatctgtt
     3001 ctgctccgga gacaagagac agtgttaggc cctgcctcgt tcggcaaagc ccgggcgcaa
     3061 accgcgaaat tgaaggggac ggtttgccgg cggattgttc cctacgagca gcccgcaaac
     3121 gcgggcattg tagacgaact tcaggtactg ttgtaggaaa tatctctcgg gttatatgac
     3181 aagttagatg ggtccgcaaa ggaggtggtt ttgaacccat tgggagtctt ggaacggcaa
     3241 ttacgttttc taatatttca aaactcctat tcaacatctc ctcatttatc aaagcacgag
     3301 gccgaacacg tcccgcaaga attgcttgat gggccgtgag cgggccggta cggtccgccg
     3361 atagtccggc gtgagcacaa atgaacggcc cgagcgcccc ctttctgcgg ggcgctcggg
     3421 ccgtatcgtt gttgatgagc ctttgggccc ttccctagaa ctcgatgcgg gcggcgaact
     3481 ggacgacacg cgcctgcggg tcgccgaggc gcgggtcgcc ggtctgcgtg atgcggccga
     3541 aggtgttggc gttgatgctc tgcaactgct gcgccgccga gatggcgaag tgcgcccggt
     3601 tgaagaggtt gaacgcctcg gcccgcagct gcaggcggac gcgctcggtg atgcggatgt
     3661 tcttgaagag cgaggcgtcg atattgaagt acatcggccc gttcaggaag aacctctcca
     3721 gctggccgaa ctcgccgggc ggcacgttga agaagatctg cccggggaac gcggccgtcc
     3781 cgaggccgtt ggcgccgcgg ctggcgccga tatcggcgca gcggccggcc gccagcgcct
     3841 cctggttgat ggtcgtcacg gccgggttga tcaggaagac gccgcacggc gtgcggaaga
     3901 cgccgaccag gtcctggatc tgatccttcg tcagcgagga gttggccgtg ttgcgcgccg
     3961 agcgggcgtt cgagccgaag gtcccgcgcg ggtcgacgat gctgaacggg gcgccggtcg
     4021 tcaggctcac gatggtgttg agctgccagc cgccgaccaa gcggtcgaac cacgggccgg
     4081 cgtcggtgaa gaagtggcgc cccttgccga agggcagttc gtagatggag ttgaagttga
     4141 agacgtgcgc cgtgtcggtc tgcgcgcgcg agtactcgag ctcgtcccgc ttcgggtaga
     4201 cctggaactc gaagcggcgc tggtcgacgc ccggcgcgtt cgagagcgtc ttctggaacg
     4261 tgtagttgcc ctggaagtag agcccctgcg cgaagcggcg gcgcacctcc atctggagcg
     4321 agttgtagtt gtagcgcgcg ctgttcgaga gcagcaggcc gaggccgatg ttcgggttcg
     4381 cgaccagcgg gaccgtgccc gacagcccgt tgccgacgta ggtggccgcg agctggccgg
     4441 gcaggcccgt cgagaccagg ttcgcgaccg tcgcgttcgg cgcggtcgtc gcgttgccga
     4501 tgccgcccgg cgtgccggag agcgtcccca ggttgatgaa gacctggagc ggctggcagc
     4561 cggggttctg cgccgtcgtg cagttgagct gccctgtcag cgcctggttc gagcgggcgc
     4621 ggaagaagtc gtcgaggaag ccgttcgtga tgagctccgg cgggttgacg tcggtgtagc
     4681 gcgtgacgtt attgctgaag gccccgacgt agcggatctc gaaggcggtc tggaacccga
     4741 tctcgcgctg gatgccgacg gtgtactcgt ggttaatcgg cagcttgatg ttcgggtcgg
     4801 tggtggccac cgactgaatg gccccgccgg cccgcgcgtt attctgcgcg aaggtgcgcg
     4861 ggatcaggac cgtcggcgtg gtgatggtgc cgagggcgcc gagccgctcg ttgaggttgt
     4921 tgcggctcgc gttctcgcgc ttgccggagt tctggtcgcc ctggcccgaa gagcccttca
     4981 ggaactcgtc gttgaagtag ctcaggcggt agccgccgcg gatgacggtc cgcccatcgc
     5041 cggggatcag cccgcggagg aacttgttcc gaaactgcgg cgagtaggcg acgctgaacg
     5101 agggcgcgaa gttgttcttg tcggtgttga agagcttgcc gccgccgacg ttctcgcccg
     5161 cggtccccag gcgcccgttc gggtcgagca gcgcctcggc caccgtcttc ccttcgggga
     5221 cgatgacctc ggcgaggacg ccgttgcgct cgcgcacggc cggccacagc tcgtaccgga
     5281 gtccgaggtt gacggtcagg ttcggccgga cccgccactg atcggagaag tagatgttgt
     5341 agttctcgta ggcgtagtcc tggcggaagc cggcgagcgg gacgagcccc tggtcggcgc
     5401 tcagcacgtt gaaggtctgt gactgctggc tgacgatgcc gccgaggaag gcgtagagct
     5461 ggttggcggt cgtgcggtcg gtcgtgttga tgccgcccgg gaagagcgcc gagttggtga
     5521 acatggccgc cgtgatctgc ggcgtgttgg tgccgacgcc gatgacgtag gtcgggagca
     5581 cgccgccgtc gttcaagcgc agcgcacggt aggcgttggc ctgcacgccg aaccgcagcc
     5641 cgtggtcgcc ccacaggtag tcggcgttgt cctggagggt gtaggtcttg gtgtggcgcc
     5701 cctggaagag gaacgtcgac tcggggttgc tgaggaagcc ggccagcgcc ccggcggcga
     5761 tgttcgtctg gctgaactgg ttcggctgcc gcccggccag ctccgggaag agctgcgcgc
     5821 tgcggacgta gccggcgcgc acctcgttgg tgaagacggg cgtgagcgtc gagcggaagg
     5881 cgaggacgta cgagtcgttc ggcgtgctga agccggacgg cggcgtcggg tcgaagccgt
     5941 tgaggtcgga gaagtcaggg acgaagccgg tcgccgtctg ccgcttgtag atggcgttca
     6001 gcgtgttcgt cgagttgatg tcgtagtcga gccggatggt catgttgtcg gcgtcggagt
     6061 ccgacttctg gttgaagcgg tagccggtgg tgttgcgctg gtcacccgcc tcgatggagt
     6121 tgccggccgg gatgttggcg aggaaccggc tctggatgac ggggttgatc ccggtcggcg
     6181 ggttgttagc cgtgatcggc agcgcgaaga ggttgacctg ccgcgtcacg cccccgttat
     6241 cgacgtaggt gaagaggccc tggcgcgccg aaggcgtcag caccgtgcgc aggctcggct
     6301 gcgagaggcg gtcgcgcgtg ccctcgtagt ggccgaagaa gaagagcttg ttcttgatga
     6361 tgcggccgct caggcgcccg ccgaactggt tgaagttgcg gaaggtgcgc gggctgcgcg
     6421 ctgcgccggc cgtcgcttgc ccggcgacca ccagcgggtc gttcgggccg aagttgccgg
     6481 cggcgttgtt gaagaaggtg ttggcagcga agcgcgagtt gcggttgtac tcgaagaccg
     6541 cgccgtggaa ctcgttctgc ccgcgcggcg tcgccagctg cacctgcgag gcgccgagac
     6601 cctgctcgcc gccggcgttc tgcgtcgtga cggtgaactc cgccacgtcg tcgctcgaga
     6661 gcgcgacctg cgtcgaggcc gacgcgctct gccggatgaa gttgtcctgg atgttgaggc
     6721 cgtcgagcgt gatgttggtg gccgacgggc gctggccgtt gatcgaggtg gcctgcgcgc
     6781 cgttgttcga ggtgccggcc tggagtgtga tgagcccgag cgggttgcgc ccgttcagcg
     6841 gcaggtcctg gatctggcgc gggctgacgg tcgtgctcag gtccgcgttg gacgcgttga
     6901 ggacgtcggt gccggcgacg acctcgaccg tctcgctgat gtagccgacc tcgagcgccg
     6961 ccgtcagtga gtagtcgcgc ccgacgtcga tcttgacggc ggaggcggtg aaagtcttga
     7021 agcccggcgc cgtgaccgtc acggtgtagg tgccggcgtc gagctgcggg acggtgaagg
     7081 agccgtcttc ggtcgagacg acggtccgct ccctgcccgt ctgcatgtcg gtgacggtga
     7141 cgttcgcgcc ggggatgaca ccgtccggcc cagagaccgt gccgacgagc cggcctgtgt
     7201 tcgactgagc ggccgctccg gccgcgaata ggcagagcgt gagacacgcc cgtagcatcc
     7261 tcttcatgga gttctcctcg aagttaaaac aggtatcctc gcgtcctcac gcgcgccgtg
     7321 cggcgcggcc gtccgcgccg cctcacgccg cgcgcccacc cggataattc agaattctgt
     7381 acggcgggcc tccgtcggcc gcgcggccgt gccgcgcgtg cgcgcgtgct gtggggtgac
     7441 tcatgtcgag taagcaaggt tgatgccaac tggaggcgcg agcacgacgc gctcgacaag
     7501 cgcgcgcctg gccggatgat tgcccctcga ggtagggcgg gccggccgcc gcgaggggcg
     7561 tgtgccccga cgtcgaatcc gaattcttga atttatgttt tcatatattt tatgaatatg
     7621 ccggcgggga cgcgcgccgg cactggtcgg cgctcgcgcc gcgcgcgtcc aacattacgt
     7681 acggccggcg ccatagcgga cgcgccgctc gttcagcatt ttgctcttcg gtacaattcg
     7741 gtggaacggt tctgatgtga tgaagcaggc acctctagct cacgagtgag ctgcggtaga
     7801 ctcagtttgc gcatgagacc gccaaggagg tgcccatgac gatccattgt ggtgtcgact
     7861 tccacgcgcg tcaacagacc gtgtgctact gcgactcagc taacggtgag gtccatctca
     7921 aggagttgga tcacgagcgg gatgatgttc gtggcttcta ttccgccttg acgggcaaag
     7981 taatcatcgg gatagaagcc agcggctata gcacctggtt cgtcgaactc gtcgagggcc
     8041 ttggccacga ggtgctcatc ggcgatgcgg ccgagatcag gcgtttagcc aagcggcggc
     8101 agaagaatga ccggcgcgac gccgggctca tcctcgacct gctgctcaag cgggagttcc
     8161 cgcaagtcca tcgcccctca ttcgagagcc gtgaagtgtt gcgactcctt cgctaccggc
     8221 acaagctggt gcaggtgcgg acgcgcgcca agaacagcct gcaagccgtg gctttcagtg
     8281 cgggctcggt cgggcggtcg aagctgctca gcaaggcggg gcgtgagcgg ttcgtcctgc
     8341 tgccgatgag cgaggcgatg atccggcagc gtgacggatg gctctcactc gtagacgacc
     8401 tcagcgcgcg catcaagagc ttggacgctt ggctcgggca gcaggctaag caagatgagc
     8461 gcgtgctgcg gttgcaaaca catccgggca tcgggctctt aacttcgctg gcgctggttc
     8521 acggcttaga gccggtgact cggttcgacg gcgggcggaa ggtcgcggct tacgtgggct
     8581 tggatccgat ggaatactct tccggggata aacagcggtt cggctcgatc agcaagggtg
     8641 gctcacggct gctacgctac ctgctggtgg aggcggcgca gatcacggtc aggcgagacg
     8701 aggggttgaa gcgtttctac ctccggctcc tccatcggcg aggtgcgcag aaggcgcagg
     8761 tggcggtcgc gcgcaagctc ttgattcgcg gctacatcct gttgcgggac gggatcgatt
     8821 atgcggagtt cctccgccgg ggcgtcgaag cacggcctgc ccggcttgca ccataggcta
     8881 gggatgcctg gggttctgat tgggcggccg gcctcccgcc cctgagcggg agtgcgaagt
     8941 aaacctcatg ggttttgaat gcccggatag taactatgat gacgaagtag ttgagcctct
     9001 tctcctgaga gcggtatgtt gtgatcgccg gatcggcaac accaactctg aaaaggagga
     9061 ccaactcatg cgagcgcaca acatcagctt gccggatgac gagactgttt tcgccggcat
     9121 cgacatgcat caacggcagt ggcgcgtcac gatcaggacc ggtgaggtgg aactcaccag
     9181 cgccagcatc cccggcgact ggcagtcgct gcgccgcttg ctagagcgat atcacgggca
     9241 tcccgttgaa gtcgtctacg aagccggctg cttcggctac tggctgcacg accgagtggt
     9301 cgagtacgga gctcagtgca tcgtgactcc acccagcctg atcccgcgcg agagcggcaa
     9361 ccgggtcaag acggacaagc gcgacagccg caagctggcg ctgctgctcg ccaaagggat
     9421 gctcaagcgg gtctatgtcc caacgccgca ggagcgtgag caccgcgcgg tcgcgcgccg
     9481 ccggaggcag ttgatccggg agcgggtgcg ggcgcagaac cggatcaagg cggaactgcg
     9541 tttctacggc ctccggctgc cggagatcac gggccggtgg tcgaagctct acgtcgagaa
     9601 cttgcgccgc atccgcttct ccacccgctg gcaacaggag agcttcgaga gcttacttga
     9661 gcagtatgag ttcttcacgc agcagatcga gcgccagacc aggctattgc gctcgctgtc
     9721 acagacagcc gagtatcgag agcgagtcaa gattctccag agcatccctg gcgtcgggct
     9781 gatcgccgcg atggagatgc tgctggagtt gcaggacgtg gcgcggttcc ggcgggccga
     9841 ccagttggcg gcttacgtcg ggctgacgcc cgcgcagcgt tcgagcggcg acgtggtacg
     9901 gatggggcgg atcacgagga gcgggaagaa ctcgttgcgc gggactttgg tggaagcggc
     9961 gtggcggatg gtgtcgagag acagcgcggt gcgccagcag tacgagcagc tcaaagcgag
    10021 ggccggggcg aagcgggcga ttgtcgccgt cgcccgccgg ctgctgttgc gggccaggcg
    10081 gatgctcctg gacaagcgag cttacgtcgc cgagttggcg gcagcctgag tgacataatc
    10141 aaagaccgac cggcatcacg gaggttggag tatggcggtc aatcatctgt gagtgccgga
    10201 tgaggcagcc acgtcggata gtgtgccagc tccagcgctg aatccttcgg atcgttacct
    10261 gtggcttcgc ggcagggcag aagaccacga atagcagatt ggttcgggtt cacatcaggc
    10321 gacataggcg gggtgggctt gactgcgtac atagcagatg attgatcgcg gcccggctct
    10381 ttgatcggac gagggacatt cgacagcaac tacgaaggtg cggtctcacg cgcggcagat
    10441 cgacaaactt ggcagtgaag acgcgggctc gtgccgtgat aagcgatgtg tgtctgcaat
    10501 agtgcttgac acctgtgtct gcttcataga tggtgcaaac cggaatgccg acaggtaatc
    10561 aggcacacgt ctcagttccc ccccaacccc gcgccttcgc cccacgcccg ccgactcacc
    10621 ccgcagactc gattccatga aacgggcgcg ttgagaa
//
LOCUS       DBNGOH010000201         3737 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3880143, whole genome shotgun sequence.
ACCESSION   DBNGOH010000201 DBNGOH010000000
VERSION     DBNGOH010000201.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3737)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3737)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3737
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3880143"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(197..514)
                     /locus_tag="AC159P_03835"
     CDS             complement(197..514)
                     /locus_tag="AC159P_03835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694616.1"
                     /translation="MARSLYGRATHALRWFLLRRLPACKEMVRVMSESMERPLSLRER
                     VLLKLHLWVCVWCVWYLEQQRLLREALRARAARPEAAASPGVSLSAEARERIKVALRR
                     PLP"
     gene            complement(518..1138)
                     /locus_tag="AC159P_03840"
     CDS             complement(518..1138)
                     /locus_tag="AC159P_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR3694617.1"
                     /translation="MIREQTAVAHSSPDPGAWVDEHGDYLFRYAMFRLRETTTAEDAV
                     QETLLAALQAYDTYAGRGSERAWLTGILKHKLTDHFRRLGRETPLSRIEGEEFEHGEF
                     FRPPGDEWEGHWAVGYTPSEWCETPESLAEQGEFFRVLDDCLSPLPARVSGAFVLREV
                     DGLGTEEICRKFGVSANNLWVMLHRARLHLRRCLELNWLKPGAVKH"
     gene            1293..1475
                     /locus_tag="AC159P_03845"
     CDS             1293..1475
                     /locus_tag="AC159P_03845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694618.1"
                     /translation="MKLKPCRDCGQMVPSDARGCPTCARNLEAERMLAKYFWGVLVPA
                     LFLLACAGVYLMYVRT"
     gene            complement(1499..2014)
                     /locus_tag="AC159P_03850"
                     /pseudo
     CDS             complement(1499..2014)
                     /locus_tag="AC159P_03850"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
     gene            2375..2614
                     /locus_tag="AC159P_03855"
     CDS             2375..2614
                     /locus_tag="AC159P_03855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694619.1"
                     /translation="MANPIGAVCEKARRSIDSYAGGELVGAALEVGRHLESCPACAAV
                     AAERERLSRLVRRAVRRDAAPESLRRRVSDAIRRG"
     gene            2630..>3737
                     /locus_tag="AC159P_03860"
     CDS             2630..>3737
                     /locus_tag="AC159P_03860"
                     /EC_number="2.7.7.-"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HIR3694620.1"
                     /translation="MEKPKSMMRLPGRAQQFEGRPADPRAAELSSGLLSALFGDERRF
                     SLEHRLFNSVSLLNAAANLGGALAAPKLSDYEFLFLLHLSTGALFLSCYCFSRFRNAY
                     RPLYWPFVLLTLGFVFTNALRNAATHGGAHYYLIPALLIAVILSERTRTTLLAVALFV
                     VATAALLFVEQLRPAWVKPFADPRERFADVAGNLLFVQLFTGALVLVLSRTLNQERRK
                     SDRLLLNVLPETVARELKETDRVTPLDYEDASVLFTDFVGFTEIAERMTPQELVGELD
                     GCFRRFDRVAKRHGLEKIKTIGDAYMAAGGVPEPNRTHAVDCVLAALGIRREMAEMTA
                     RRRAAGRPHWQLRIGINTGGMVAGVVGSEKFAYDV"
BASE COUNT          603 a         1237 c         1251 g          646 t
ORIGIN      
        1 ttctcaacgc gcccgtttca tggaatcgag tctgcggggt gagtcggcgg gcgtggggcg
       61 aaggcgcggg gttggggggg aactgagacg tgtgcctgat tacctgtcgg cattccggtt
      121 tgcaccagag ccataccgag cgggtgaagg cggagctgtc cgaaaggggg cgcgtaggtc
      181 tcgtgggatc gccgggctaa ggcagcgggc ggcggagcgc gactttgatg cgctcgcgcg
      241 cttccgccga gagcgagacg ccgggcgacg ctgcggcctc gggtctggcc gcccgcgcgc
      301 gcaacgcttc ccgcaggagg cgttgctgtt cgagatacca cacacaccag acgcagaccc
      361 agagatggag cttgagcagc acgcgctcgc ggagcgagag cgggcgctcc atggactcgg
      421 acatcacccg caccatctcc ttgcaggcgg ggagccgccg gagcagaaac cacctgagcg
      481 cgtgcgtcgc cctgccgtaa agactccgtg ccatcgccta gtgtttgacc gctcccggct
      541 tgagccagtt gagttcgagg cagcgccgca ggtgcagacg cgcgcggtga agcatcaccc
      601 agaggttgtt cgccgagact ccgaacttcc ggcaaatctc ctcggtgccg aggccgtcca
      661 cctcgcgcag gacgaaggcg ccgctgacgc gcgccggcag cggcgacagg cagtcgtcga
      721 ggacacggaa gaactcgccc tgttcggcca gcgactccgg ggtctcgcac cactccgacg
      781 gcgtgtagcc gaccgcccag tgtccctccc actcgtcgcc cggcggcctg aagaactcgc
      841 cgtgctcgaa ctcttcgcct tcgatccggc tcaagggcgt ctcgcgcccg agccggcgga
      901 agtgatccgt cagtttgtgc ttgaggatac ccgtcagcca ggcccgctcc gatccgcggc
      961 ccgcgtatgt gtcgtacgct tggagcgccg ccagaagcgt ctcctggacg gcgtcctcgg
     1021 cggtggtagt ctcgcgcaga cgaaacatcg cgtatctgaa gaggtagtcc ccgtgctcgt
     1081 caacccacgc gccggggtcg ggggaggagt gtgctacggc tgtctgttct cggatcattg
     1141 aaaccggctt cggcgggctg gcctgtggcg gacgcagcgg gggaagcgcc tccgattcgc
     1201 gacttcttcg tgctgaaaaa gatcgacctc aagagttcgc tggtatatta gcacagtcgt
     1261 tccaacagcc tccggaggat gcgggccaag cgatgaagct caaaccctgc cgcgactgcg
     1321 gccagatggt gccgtccgac gcgcgcggct gccccacctg cgcgcgcaat ctggaagccg
     1381 agaggatgct cgcgaagtac ttctggggcg tgctcgtccc ggctctcttc ctcctcgcgt
     1441 gcgcgggcgt ctacctcatg tacgtgagaa cctaagtatc gggaggcgca cgttcgttct
     1501 agacgtgagc caactgcctg atgccgtact gaacggccac gcccgccaga gcctctcgta
     1561 actgtctcct gccgcggctc agtctggaca tcacggtgcc gatgggaatg ccgagcactt
     1621 ccgcgatctc cctgtaggtg aaatcttcca cgtcggcgag cagcaccacc tcgcggtagt
     1681 tcagcgggag tccgtccacg gcctcgatga cctcctcgtc ggtcaggtcc tggcggacgg
     1741 gcggctcgta ggcgacggtc tcggcgatga gcgcgtcgtc ggcccggagc aagccgcccc
     1801 ggaagtattt ccgtcggcgg tggtggttcg tcttgtggaa gagaatcttg tagagccacg
     1861 cgcggcagtt ggtgcccggc tcgtacttgt ggaacgactt ccacgcctgc aagtacacat
     1921 cctgcacgag gtcttcggcc tcggtgcgat cccgcacgag gcgcacggcc atccggtaca
     1981 ggtcgtcgag gtggggcagg gcttcggact cgaactcttc gactgtaaac attctctcca
     2041 cggggatgcg ctccttctca agctcgacgg aagtctttcc gaatgattcg gacgccctcg
     2101 ggtcaactgc tcacacccat tggtcgcaag cgcggcggaa tccttacagg gaaaattttt
     2161 ttgcaggcgg aaggcgcgcc ctgcttggga aggcttgttc gccttcgcgc cgggctgtgg
     2221 ctgctgcccg aggcccgaag caaaaaggcc gcgggcaagt cggggaataa atcttgggcc
     2281 ggcggtgatt aaacagacgt tgacggggcg gacgcgtgcg agccgaagct gtgtcggcac
     2341 atcgcaccgt gcggggcgtc agggagtgta cagaatggca aatcctatag gcgcggtctg
     2401 cgagaaggca cgccggtcga tcgattccta cgccggaggc gaactcgtcg gcgcggcact
     2461 ggaagttggc cggcacctcg aatcgtgccc cgcctgcgcc gccgtggcgg cggagcggga
     2521 gcgcctcagc cggttggttc ggcgcgccgt ccgccgcgac gcggcccccg aaagtctgag
     2581 gaggagggtc agcgatgcga ttcgcagggg ttgagcgccg gacaacaccg tggagaaacc
     2641 gaagagcatg atgcgtctgc cgggccgcgc gcagcagttt gaaggccgtc ccgcggaccc
     2701 acgggcggcg gagttgtcga gcggcctgct ctccgccctc ttcggcgacg agcggcgctt
     2761 ctcgctcgaa caccgcctgt tcaatagcgt cagcctgctc aacgccgcgg cgaacctcgg
     2821 cggggcgctc gccgcgccga agctcagcga ctacgaattc ctcttcctgc tgcacctctc
     2881 gacgggcgcg ctcttcctct cctgctactg cttctcgcga ttccgcaacg cctaccgccc
     2941 gctctactgg ccgttcgtcc tgctgacgct cggcttcgtc ttcacgaacg cgctccgcaa
     3001 cgcggcgacg cacggcggcg cacactacta cctcatccct gcgctcctca tcgccgtcat
     3061 cctctcggag cgcacgcgca ccaccctttt ggccgtcgcc cttttcgtcg tggcgaccgc
     3121 cgccctcctg ttcgtcgagc agctccgccc ggcgtgggtc aagcccttcg ccgacccgcg
     3181 cgagaggttc gcggacgtgg cgggcaacct cctcttcgtg cagctcttca ccggcgcgct
     3241 cgtgctggtg ctgtcgcgca cactcaacca ggagcggcgc aagtccgacc gcctcctgct
     3301 caacgtcctg cccgaaacgg tcgcgcgcga gctgaaggag accgaccgcg tgacgccgct
     3361 cgactacgag gacgcgtccg tgctcttcac agacttcgtc ggcttcacag agatcgccga
     3421 gcgcatgacg ccgcaggagc tggtcggcga gctggacggg tgtttccgcc gtttcgaccg
     3481 tgtggcgaag agacacgggc tggaaaagat caagaccatc ggcgacgcgt acatggcggc
     3541 ggggggcgtc cccgagccga acaggacgca cgccgttgac tgcgtgctgg ccgcgctcgg
     3601 catccggcgc gagatggccg agatgacggc gcgccggcgc gccgccggtc gcccgcactg
     3661 gcagcttcgc atcggcatca acacgggcgg gatggtggcc ggcgtggtcg ggagcgagaa
     3721 gttcgcctac gacgtgt
//
LOCUS       DBNGOH010000202         2671 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1680407, whole genome shotgun sequence.
ACCESSION   DBNGOH010000202 DBNGOH010000000
VERSION     DBNGOH010000202.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2671)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2671)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2671
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1680407"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..207)
                     /locus_tag="AC159P_03865"
     CDS             complement(<1..207)
                     /locus_tag="AC159P_03865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694621.1"
                     /translation="MKKFYSLFAAIILAFAALSVVAAPASAREGKGEKRSSAARLSVP
                     LNLAILIQDDLVSRVGNEIGVTREF"
     gene            568..1419
                     /locus_tag="AC159P_03870"
     CDS             568..1419
                     /locus_tag="AC159P_03870"
                     /inference="COORDINATES: protein motif:HMM:NF018473.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1223 domain-containing protein"
                     /protein_id="HIR3694622.1"
                     /translation="MNKLLAWVVAAVVVAVFLFAVGALRRGGATTSRAAKEEKAGEAP
                     APGGAARVPVLVELFTSEGCSSCPPADALLSRLEKTQPVEGVEVIALAQHVDYWNYLG
                     WRDPFSNAEAGARQYEYAKAFEKDGVYTPQMVIDGRTEFPGGKTALAFETIAKAARTP
                     KAEVFVATLPDGENGAKGVRLSVRVGKLPPLTAGDTADVLLAITESDLATSVGRGENA
                     GRRLGHVGVARTLSVIGAAAGETFKAEPSVSIDGSWRRENLRAVVFVQERASKRVLGA
                     ASIKLVG"
     gene            1569..2555
                     /gene="msrP"
                     /locus_tag="AC159P_03875"
     CDS             1569..2555
                     /gene="msrP"
                     /locus_tag="AC159P_03875"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HIR3694623.1"
                     /translation="MTSKKADDIKSSEITDKKIYLSRRVFMRGAALAASTAATGCLYR
                     KLLAPEAQLANPPGGAAPVAQASAGRWALNGEEATSYQDITHYNNFYEFGTDKYSPAR
                     RAQRLVTRPWTVAVDGLVNKPKVFDLDELLRLAPQEDRVYRLRCVERWSMIIPWLGFP
                     LAALLKQVEPQGSAQYVAFETANDRAKNIPATSEFESGLDWPYVEGLRLDEAMHPLTI
                     LATGIYGEPLLPQNGAPVRLVVPWKYGYKSIKSIARISLVERVPPTAWNRAAPNEYGF
                     YSNVNPEVDHPRGSQAMERRIGEYTQRPTLLFNGYADQVAHLYAGMDLRYYS"
BASE COUNT          467 a          873 c          905 g          426 t
ORIGIN      
        1 gaattcgcgc gtgaccccga tctcgttgcc gacgcgcgag accaggtcgt cctgaatcag
       61 gatcgccaaa ttaagaggca cgctcaagcg cgccgccgag gagcgcttct cgcctttccc
      121 ttcgcgcgcg gatgcgggcg cggccaccac gctcagggcg gcgaaagcga ggatgattgc
      181 cgcgaacagg gaataaaact tcttcattgt ctgacttcct tcctcccgcc gttcggctct
      241 gccgaacgtg cgttgtccgt ttccgattcg tcccctgaga gggacatttc atgcgctact
      301 gagatgcccc ttgcgcctta aacgtggcct gcggcctcgg ggcgcggggg cgcggcgcgg
      361 cgacacgcgc cccctctctt cactcaagaa cgcgggaata aaacggcgcg acggggtatt
      421 atttactttg agatgatgcg cccccgcgac ccttcatgaa gccgccgttg acgagccgcc
      481 gcgggcacgc cgcgcggctg aggcaacgcg cgcgcgcgcg gcaggctcaa actcgcacgg
      541 tcgtttattt agacgagggg gagactgatg aataagttat tagcgtgggt cgtcgccgcc
      601 gtcgtggtgg cggttttcct gttcgccgtc ggcgcactga ggcgcggcgg cgccactacc
      661 tcgcgcgccg cgaaggaaga gaaggcgggc gaggctccgg ccccgggcgg ggcggcgcgc
      721 gtccccgtgc tggtcgagct gttcacgtcc gaaggctgtt cgagttgccc gcccgccgac
      781 gcgttgctct cgcgccttga gaagacgcag ccggtcgagg gcgtggaggt gatcgcgctc
      841 gcgcagcacg tcgattactg gaactacctc ggctggcgcg acccgttctc caacgccgag
      901 gcgggcgcgc ggcaatacga gtacgcgaag gcgttcgaga aggacggcgt ctacacgccg
      961 cagatggtca tcgacggtcg gacggagttc cccggcggca aaacagcgct ggccttcgag
     1021 accatcgcca aagcggcgcg cacgccgaag gccgaggtct tcgtcgcgac actccccgac
     1081 ggcgagaatg gggcgaaggg cgtgcgcctg agcgtgcgcg tcgggaagct gccgccgttg
     1141 acggcgggcg acacggcgga cgtgctgctg gcgattacgg agagcgacct tgcgacgagc
     1201 gtcgggcgcg gcgagaacgc ggggcgccgg ctcggccacg tcggcgtcgc gcgcacgctc
     1261 agcgtcatcg gcgccgcggc gggcgagacg ttcaaggcgg agccgtcggt ctccatcgac
     1321 ggcagttggc ggcgcgagaa tttgcgtgcg gtcgtcttcg tgcaggagcg cgcgagcaag
     1381 cgcgtgctcg gcgcggcgtc gatcaagctg gtcgggtagg gcgcgggcgc ggaagggggc
     1441 ggaggccgga cgcgccggaa tccgctcccg cccgccccat acgtaatgtc cacgctccgt
     1501 cgagcgtgac cgccgcgggg cggccacgcg gatttcaagg cgacggtttc gacaggcgac
     1561 gtgttcttat gacgagcaag aaagccgacg acatcaagag cagcgagatc accgacaaga
     1621 aaatttacct gagccgacgc gtcttcatgc gcggcgcggc gctcgcggcc tcgaccgccg
     1681 ccacgggctg tctctaccgc aagctgctcg cgcccgaggc gcagttggcg aaccctccgg
     1741 gcggcgcggc gcccgtcgcg caggcgtcgg ccgggcgctg ggcgctgaac ggcgaagagg
     1801 ccacgagcta tcaggacatc acgcactaca acaacttcta cgagttcggc acggacaagt
     1861 acagccccgc gcgccgcgcg cagaggctcg tcacgcgccc gtggacggtg gccgtcgatg
     1921 gtctcgtcaa caagcctaaa gtcttcgacc tcgacgaact cctgcgcctc gcgccgcagg
     1981 aggatcgcgt ctaccgcctg cgctgcgtcg agcgctggtc gatgattatt ccgtggctcg
     2041 gcttcccgct ggcggcgctc ctgaagcagg tcgagccgca aggctccgcg cagtacgtcg
     2101 ccttcgagac ggccaacgac cgcgcgaaga acatccccgc cacgagcgag ttcgagagcg
     2161 gcctcgactg gccctacgtc gaaggacttc gtctcgacga ggcgatgcac ccgctgacga
     2221 ttctcgccac gggcatctac ggcgagccgc tgctgccgca gaacggcgcg cccgtgcgtc
     2281 tggtcgtgcc gtggaagtac ggctacaaga gcatcaagtc catcgcccgc atcagcctcg
     2341 tcgagcgcgt gccgccgacc gcctggaaca gggccgcgcc caacgagtac ggcttctact
     2401 cgaacgtcaa ccccgaggtg gaccacccgc gcgggagcca ggccatggag cgccgcatcg
     2461 gcgaatacac gcagcgcccc acgctcctct tcaacggcta cgccgatcag gtggcgcatc
     2521 tttatgcggg gatggatttg cggtactact cttaggaaac gatgaatgcg gaatgctgaa
     2581 cgatgaatag aagacaagct gagtgataaa aggcaccgtg cttttcattc atcattcatc
     2641 aatccgcatt catcatttgt tctatgcggg a
//
LOCUS       DBNGOH010000203         3949 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2046808, whole genome shotgun sequence.
ACCESSION   DBNGOH010000203 DBNGOH010000000
VERSION     DBNGOH010000203.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3949)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3949)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3949
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2046808"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2507
                     /locus_tag="AC159P_03880"
     CDS             <1..2507
                     /locus_tag="AC159P_03880"
                     /inference="COORDINATES: protein motif:HMM:NF028442.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HIR3694624.1"
                     /translation="GSDFRTLVTLPGAQLYRGGVARTFAFPETAARLYRIEMTGAPLR
                     PAEVMSQAQTQPAREYTLSEAALHAGARVHRWEEKAGFSFLFEYESVPTPPAPADASI
                     RSADVIDLTAKMKADGTLDWEVPPGRWTVMRFGYSLTGAKNRPAVPAGLGYEVDKLSR
                     KHLEAYLRGYTEPISGALGELYGKSLRYVLLDSWEAGMQNWTEELLAEFRRRRGYDPV
                     PYLPALAGRVVGSAEVSDRFLWDFRRTLADMYAENHYAAATEFLHRQGIDTYSEAAGV
                     SLEIPEDTLLNKKHVDIPVGEFWVRDLHPPAMYYEDVRGAASAAHVYGKGIVAAESFT
                     GGGYESPFALKKIADYWFAQGVNRIIFHTSAHQPLDTKPGNTMVGTHINRNITWAEQA
                     RPFMIYLARNSYMLQRGLPVADLAYLLNEGAPSTMPFWGAGLKPAPPAGYDYDYVNAD
                     VLLSRMTVGADGRLLLPDGVSYRVLVLPEVDRMTVPVLRKIRELVSGGATVLGPKPAK
                     SPSLGGYPAADGELLAMASDLWGDLDGVSRTRRVYGKGMVVWGLPPSEVLAALRVPRD
                     VEYSRGLDADIAWIHRREGDADIYFVANRADRAQDVEVRFRVSGKEAELWHPDTGTSE
                     PAGYVIADNRTTVPLHLEEREAVFVVFRRPTSSPSRVVTRANPSPLAALAGPWDVGFP
                     PNLGAPAKLRLAELVSWTANTDEGVKYFSGTATYTKTVQAPRGWFRTGARILLDLGAV
                     KDIAEVSLNGKALGVLWKPPFRVDVTGALKPGANRLEIKVTNQWTNRLVGDRAAPADK
                     KVLAAEASAARGFGPPPALTESGLLGPVTFISAEVR"
     gene            2546..3859
                     /locus_tag="AC159P_03885"
     CDS             2546..3859
                     /locus_tag="AC159P_03885"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014784786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HIR3694625.1"
                     /translation="MRHESWWMVASLTAALSLSLSAASGQRQNAPPTNVAGIPVNYDE
                     SLTGNYTLPDPLVLADGKRVRDANTWNKKRRPEIVRLFEEHQFGRSPVRPAGMSFDVF
                     DKGTPALGGKALRRQVTVYFSPDKAGPKMDLLVYLPAGARGAVPLLLNLSFTANSNVV
                     DDPGIKPGEVWGRDRKRTPAPKGTNFGRLDVLPFLASGIGVATVYYGDIDPDFPGGIE
                     HGVRSLYLKQGQTEPAPDKWGTIAAWGWGLSRALYYFETDKGVDAGRVAIVGASRLGK
                     TVLWAAARDTRFAMVIASCSGEGGAALSRRHFGETVKHLTAPSRYPYQFAANYQKHAD
                     RVGESPFDAHMLVALIAPRPLLLQTGDTDLWSDPKGEFLSAVAAGPVYRLLGRQDLGT
                     NEIPPAGQPILHTLGYYMHAGGHGTIPSDWEQFLKFMQSHLLRAR"
BASE COUNT          652 a         1325 c         1367 g          605 t
ORIGIN      
        1 acgggtcgga cttcaggacg ctcgtgacgc tgcccggcgc gcaactttat cgcggcggcg
       61 tggcgcggac gttcgcgttc ccggagaccg ccgcgcgtct ctatcggatt gagatgacgg
      121 gagcaccctt gagaccggcg gaggtgatga gtcaggcgca gacacaaccg gcccgcgagt
      181 acacgctgtc ggaggcggcg ttgcacgcgg gcgcgcgcgt ccaccgctgg gaggagaagg
      241 cgggcttcag cttcctcttc gagtacgagt cggtgccgac accgcccgcg cccgccgacg
      301 cgtcgattcg gagcgcggac gttatcgacc tgaccgcgaa gatgaaggcg gacggcacac
      361 tcgactggga agtgccgccc ggcaggtgga cggtcatgcg cttcggctac tctttgacgg
      421 gcgcgaagaa ccgcccggct gtgcccgccg gactcggcta cgaggtggac aagctcagcc
      481 ggaagcatct ggaagcctat ctgcgtggct acaccgaacc catctccggc gcgctcggcg
      541 agttgtacgg caagagcctg cgctacgtgc tgctcgatag ctgggaggcg ggtatgcaga
      601 actggacgga ggagctgctc gccgagttcc ggcggcggcg cggctacgac cccgtgccgt
      661 atctgcccgc gctcgcgggc cgcgtcgtcg ggagcgcgga ggtgagcgac cgcttcctct
      721 gggacttccg ccgcaccctc gcagacatgt acgccgagaa ccattacgcc gcggcgaccg
      781 agttcctgca ccggcaaggc atcgacacat acagcgaggc ggcgggcgtc tcgctggaga
      841 tacccgagga cacgctgctc aacaagaagc acgtggacat ccctgtcggc gagttctggg
      901 tgcgcgacct ccacccgccc gcgatgtact acgaagacgt gcgcggcgcc gcctcagccg
      961 cgcacgtcta cggcaagggc atagtcgccg cagagtcgtt caccgggggc ggttacgagt
     1021 cgccgttcgc gctgaagaag atcgccgact actggttcgc gcagggcgtc aaccgcatca
     1081 tcttccacac ctccgcgcac cagccgctcg acaccaagcc cggcaacacg atggtcggga
     1141 cgcacatcaa ccgcaacatc acctgggccg agcaggcacg cccgttcatg atctacctgg
     1201 cgcgcaactc ctacatgctc cagcgcggcc tgccggtggc cgacctcgct tatcttttga
     1261 acgagggcgc gccttcgacg atgcctttct ggggcgcggg tctgaagccg gccccgcccg
     1321 ccggttacga ttacgactac gtgaacgcgg acgtgctgct gagccgcatg acggtcggcg
     1381 cggatggtcg gctcctgttg ccggacggcg tgagctaccg cgtgctggtg ctgcccgaag
     1441 ttgatcgcat gacggttccg gtgctgcgca aaatccgcga actcgtcagc ggcggcgcga
     1501 ccgtcctggg gccgaagcct gcgaagtcgc cgagcctcgg cgggtatccc gcggcggacg
     1561 gggagctgct ggcgatggcc tccgatttgt ggggcgacct cgacggcgtc agccgcacca
     1621 ggcgcgtcta cgggaagggg atggtggtgt gggggctgcc gccttcggaa gtcctcgccg
     1681 cgctgcgcgt cccgcgcgac gtggagtaca gccgcgggct ggacgcggac atcgcgtgga
     1741 tacaccggcg cgaaggggac gcggacatct acttcgtcgc gaaccgcgcc gaccgcgcgc
     1801 aggacgtgga ggtgcgtttc cgcgtgagcg gcaaagaggc ggaactctgg cacccggaca
     1861 cgggcacgag cgagccggcg ggttacgtta tcgcggacaa tcggacgacg gtgccgctgc
     1921 accttgagga gcgcgaggcg gtgttcgtgg tctttcgccg cccgacctcc tcaccgtcgc
     1981 gcgtcgtcac ccgcgcgaac ccttcgccgc tcgcggcgct ggccgggccg tgggatgtcg
     2041 gcttcccccc gaacctcggc gcgccggcca agcttcggct cgcggagctg gtctcctgga
     2101 cggcgaatac cgacgagggc gtgaaatatt tctcgggcac ggccacctac acgaagacgg
     2161 tgcaggcgcc gcgcggctgg ttccgaacgg gcgcgaggat tctgctcgac ctcggcgcgg
     2221 tgaaggacat cgccgaagtc tcgctcaacg gcaaggcgct cggcgtgttg tggaagccgc
     2281 cgttccgtgt ggacgtgacc ggcgcgctga agcccggcgc gaaccgtctg gaaatcaagg
     2341 tgacgaacca gtggacgaac cggctggtcg gagatcgtgc ggcgcccgcc gataagaagg
     2401 tgctggccgc ggaagcctcc gccgcgcgcg gcttcggccc gccgccggcg ctaaccgaat
     2461 cggggctgct cggccccgtg actttcatct cggcagaggt tcgctgaggc gcgcggctgg
     2521 acggacatat ggagcgttga atccgatgag acacgaatca tggtggatgg tcgcgtcact
     2581 gactgcggcg ctctcactgt cgctgtctgc cgcgagcggc cagcgtcaga acgcgccgcc
     2641 cacgaacgtg gcgggcatcc cggtcaacta cgacgagtcg ctgacgggta actacaccct
     2701 gcccgacccg ctcgtcctcg ccgacggcaa gcgcgtgcgc gacgccaaca cgtggaacaa
     2761 aaaacggcgc cccgagatcg tgcgactctt cgaggagcac cagttcggac gcagccccgt
     2821 ccgccccgcc ggcatgagct tcgacgtgtt cgacaagggc acgcccgcgc tcggcggtaa
     2881 agccttacgg cggcaggtga cggtctactt ctcgccggac aaagccgggc cgaagatgga
     2941 cttgctcgtc tacctgccgg cgggtgcgcg cggagccgtc ccgctcctgc tcaacctgag
     3001 cttcaccgcc aactcgaacg tggtggacga tcccggcatc aagcccggcg aggtctgggg
     3061 gcgggacagg aagcgtacgc ctgcgccgaa gggcacgaac ttcgggaggc tcgacgtgtt
     3121 gcccttcctc gcgagcggca tcggcgtggc caccgtctac tacggcgaca tcgacccgga
     3181 ctttcccggc ggcatcgagc acggcgttcg ctcgctctac ctcaagcagg ggcagacgga
     3241 gccggcgccc gacaagtggg gcacgattgc ggcgtggggc tgggggctga gccgtgcgct
     3301 gtactacttc gagaccgaca agggcgtgga cgccgggcgc gtcgccatcg tcggggcgtc
     3361 gcggctcggc aagacagtgc tctgggcggc ggcgcgcgac acgcgcttcg cgatggtcat
     3421 cgccagttgc tcgggcgagg gcggcgccgc cttgagccgt cgccatttcg gcgagaccgt
     3481 caaacacctg acggcccctt cgagataccc gtatcagttc gcggccaact accagaagca
     3541 cgccgaccgc gtgggcgagt cgccgttcga cgcgcacatg ctcgtcgcgc tcatcgcccc
     3601 tcgcccgctg ctgctgcaaa ccggtgacac agacctctgg tcagacccga agggagagtt
     3661 cctgtccgcg gtcgccgccg ggccggtcta ccggctgctc ggcaggcagg acttaggcac
     3721 gaacgagata ccgcccgccg ggcaacccat ccttcacacg ctcggctact acatgcacgc
     3781 cggggggcac gggacgatcc cgtccgactg ggaacagttt ctgaagttca tgcagtctca
     3841 cctcctgcgc gcgcggtaag ataaagaggg cgaggccgcc gcgctcgacg gagtcgctag
     3901 agcgaatcga cgttgcgtgt gccgcgcact catttcaacg cggaggacg
//
LOCUS       DBNGOH010000204         1682 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1314091, whole genome shotgun sequence.
ACCESSION   DBNGOH010000204 DBNGOH010000000
VERSION     DBNGOH010000204.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1682)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1682)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1682
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1314091"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1065
                     /locus_tag="AC159P_03890"
     CDS             <1..1065
                     /locus_tag="AC159P_03890"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017733883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR3694626.1"
                     /translation="MGYDTLDIIPERQWPYWANLAYGVTTTHDPSAATQSVFAQSEMV
                     KAGVMTGPRVFSTGYILYGAENPEKSVVNSYDDARGHLERLQAVGAFSVKSYNQPRRD
                     QRQRIIRAARDLRMMVVPEGGSSYQYNMTHILDGHTGIEHAIPVAPLYKDALTLFARS
                     KTGYTPTLIVGYGGIWGENYWYQKTNVYENAKLLRFTPRKVIEPRARRRMLVGEDDFY
                     HFELAKTVKDLVRAGGKAQLGAHGQLQGLGAHWELWMLQQGGLTPHEALRAATLHGAE
                     YLGLGGDIGSIEPGKLADFMITDKNPLADIRNSETVSHVMINGVLYETSNMDEVYPQA
                     RPRAPFFWERRENGTATEQR"
     gene            1153..1605
                     /locus_tag="AC159P_03895"
     CDS             1153..1605
                     /locus_tag="AC159P_03895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694627.1"
                     /translation="MRKLVQSTYDDDPRFARARSRRTTPTPPAPPAREFCYVEPSRKA
                     RREGFEGQRSGLAYAAGYAGLAYLFRYSAQTVLITTIGLDSIALMLTFAWGLCAPLAL
                     LLGLRAGRMLDRTHGLQGRLPALTGFTIGIVGTINLCGELLTWLWVSK"
BASE COUNT          313 a          590 c          538 g          241 t
ORIGIN      
        1 atgggctacg acacgctcga catcatcccc gaacgccagt ggccctactg ggccaacctc
       61 gcctacggcg tgacgacgac gcacgacccg tcagccgcga cgcagagcgt cttcgcgcag
      121 agcgagatgg tcaaggcggg cgtgatgacg gggccgcgcg tcttctccac cggctacatc
      181 ctctacggcg cggagaaccc cgagaagtcg gtcgtcaact cgtacgacga cgcgcgcggc
      241 cacctcgaac gcctacaggc ggtcggcgcc ttctccgtca agagctacaa ccagccgcgc
      301 cgcgaccagc gccagcgcat catccgggcc gcgcgcgacc tgcggatgat ggtcgtcccc
      361 gagggcggct cgtcctacca atacaacatg acgcacatcc tcgacggcca caccggcatc
      421 gagcacgcca tcccggtcgc cccgctctac aaggacgcgt tgacgctctt cgccagaagc
      481 aagacgggtt acacgccgac gctcatcgtc ggctacggcg gcatctgggg cgagaactac
      541 tggtatcaga agacgaacgt ctacgagaac gcgaagctgc tgcgcttcac gccgcgcaag
      601 gtgatcgagc cgcgcgcgcg ccgccggatg ctcgtcggcg aggacgactt ctaccacttc
      661 gagctggcga agaccgtgaa ggacctcgtc cgcgcgggcg gcaaggcgca gctcggcgcg
      721 cacgggcaac tgcaaggact cggcgcgcac tgggagctgt ggatgctgca acaggggggg
      781 ctgacgccgc acgaagcctt gcgcgccgcg acactccacg gcgcggagta cctcggcctc
      841 ggcggcgaca tcggctccat cgaaccgggc aagctcgccg acttcatgat taccgacaag
      901 aacccgctcg cggacatccg caacagcgag accgtcagcc acgtcatgat caacggcgtc
      961 ctctacgaga cctcgaacat ggacgaagtc tacccgcagg cccgcccgcg cgcgcccttc
     1021 ttctgggagc ggcgcgagaa cggcacggcc accgaacagc gttgaagcgg cgggcgggcg
     1081 ttcggcgggc cgcaggccgc cggcgccacc tgcgcggaac ttccgcggcg cccgcgcgtg
     1141 taagacggca cgatgaggaa gctcgtgcaa agcacttacg acgacgaccc gaggttcgcg
     1201 cgtgcgcgct cgcggcggac tactccgacg ccgcccgcgc cgcccgcgag ggagttctgt
     1261 tatgtcgagc cgagccgcaa ggcccgccgc gagggcttcg aaggtcaacg ctccggtttg
     1321 gcatacgccg ccggttacgc gggcttggcc tacctcttcc gctactcggc gcagaccgtc
     1381 ctcatcacaa ccatcggcct cgacagcatt gcgctcatgc tcactttcgc atggggtctc
     1441 tgcgcgcccc tcgccctctt gctcggcctg cgggcaggcc ggatgctcga ccgcacgcac
     1501 ggcctacagg gcagactgcc cgctctcaca ggcttcacca tcggcatcgt cgggacgatc
     1561 aacctctgcg gcgaactgct cacgtggctg tgggtaagta aatagtgggc agcgggcagt
     1621 tggcagttgg cggtagaaga aaaaaggcga agaggcttgg gaggtaaagt cccaagcctc
     1681 tt
//
LOCUS       DBNGOH010000205         5629 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2780483, whole genome shotgun sequence.
ACCESSION   DBNGOH010000205 DBNGOH010000000
VERSION     DBNGOH010000205.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5629)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5629)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5629
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2780483"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..420)
                     /locus_tag="AC159P_03900"
     CDS             complement(<1..420)
                     /locus_tag="AC159P_03900"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF012695.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HIR3694628.1"
                     /translation="MQALILAGGKGTRLRPLTVYTPKPVVPICNRPFLLYQIDTLRRA
                     GITDITLSLSYQPLKIEQQLGDGSDYGVKIAYTVEPQPMGTDGAYKFAEDLIREPTVV
                     FNGDILTDLDLGAVIREHEARGAAATIVLTPVENPSAY"
     gene            675..1865
                     /locus_tag="AC159P_03905"
     CDS             675..1865
                     /locus_tag="AC159P_03905"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HIR3694629.1"
                     /translation="MPENREAVIISAARTPTGKFQGALKDFTATQLGALVVREAVRRA
                     GVAPEDVDEVILGCVIQAGLGQNPARQAALNGGIPFGVSAVTINKVCGSGLKSVMMAA
                     QGIRLGDSETVVAGGMESMSNAPYLLPKAREGYRMGNGTLVDSMINDGLWCAFENYHM
                     GNTGEVVAERYGVTRAEQDEYALNSHRKAAAAQKAGKFKDEILPVEIPQKKGAAIIFD
                     ADESVREDSSMEALGKLKPAFKKEGGTVTAGNAPGVNDGASALVVTSLERARALGVEP
                     MARIAAQATSGIQPELVMMAPVEAIRKVVKKAGWSLNEVDLIELNEAFAVQAVAIMRE
                     LELDPQKVNVNGGAVALGHAIGQSGARLLTTMLYEMKRRDARRGLAALCLGGGNAVAL
                     AVER"
     gene            1949..2455
                     /locus_tag="AC159P_03910"
     CDS             1949..2455
                     /locus_tag="AC159P_03910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694630.1"
                     /translation="MTPTDHNKVLSILHLIYGGLNFISLLIVIPFFLIGIPFLGSGSG
                     EGAVLGAVFMFTALLIGFFALLFSLPPLIAAYGMHKHRPWARTAGIVAAVFAAISFPL
                     GTALCVYSFWFYFGNEGRAYYEGAGASPHWRGALGDAPASTYGWDAQRAAGERAYTPP
                     PPPPSWRD"
     gene            2485..3330
                     /locus_tag="AC159P_03915"
     CDS             2485..3330
                     /locus_tag="AC159P_03915"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014812418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HIR3694631.1"
                     /translation="MSEIIGVIGAGTMGNGIAQVGARAGYEVVMRDVEDEFLRRGLAA
                     IDRSLQRDVDKERLTEEEKRAVLGRIRTTKKLQDLAGASFVVEAITENLDAKRDVFRA
                     LDELIGPEAILASNTSSISITKLGAATKRPDKVIGMHFMNPVPVMKLVEVIRGIATSD
                     ETYEKTRALCERMGKTALDCNDSPGFVSNRVLMPLINEAIFALHEGVATRESIDGIMK
                     LGMNHPMGPLTLADFIGLDVCLAIMNVLHAELGDPKYRPCPLLRKYVDAGWLGRKSGR
                     GFYEY"
     gene            3453..4697
                     /locus_tag="AC159P_03920"
     CDS             3453..4697
                     /locus_tag="AC159P_03920"
                     /inference="COORDINATES: protein motif:HMM:NF025910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3694632.1"
                     /translation="MPVGRGQIFCQRCLAANPVERDLCERCGTRLMLVVEPAAHRFEE
                     DSGACDYEEHLLERVTVLEHHLTRFAEKLEKTLDLLLRQARSSYMDHALVESLIALLA
                     EAGTINRQTLITVWREMCERENTPALAAGRADAARDRVLAHGAGGGGVPERFERLVRE
                     GFRRVGEGDRAGGVRELEKAAALAPTNTPLHAFLGEYFFGEGKTALARDYLSLALAEP
                     EAGDWRVRLLLGLACGDEGDARRAKELLHEAIARGGPSFAAHYALGRLLAAESDWKSA
                     LAEFKRALGARECPEAHYVLGLVYYELERYPTALRHLTKAVKTSPDYGEAFYLLGLVR
                     LRLGQKEQACEAFDAAQALDREEPRYRAARRAAGRRGALPPPAPALVSTKGRARRGLV
                     TGGDLRLAAALREDALGDSPSR"
     gene            4809..5333
                     /locus_tag="AC159P_03925"
     CDS             4809..5333
                     /locus_tag="AC159P_03925"
                     /inference="COORDINATES: protein motif:HMM:NF014657.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceD family protein"
                     /protein_id="HIR3694633.1"
                     /translation="MQIEVERLDERGEAFAHTYSENELSLEDELARLVSGASVQGNAT
                     RKGDEVKLRGTLSAEVEVYCDRCAAAIVAPLEIEFDTSFLPAEVEKAAVENVELQKDD
                     LIRSVYEGGAVDLDELVREQILLALPTRSLCREECKGLCPECGANLNAGECDCARKEI
                     DPRWAALADLKKDE"
     gene            5461..>5629
                     /gene="rpmF"
                     /locus_tag="AC159P_03930"
     CDS             5461..>5629
                     /gene="rpmF"
                     /locus_tag="AC159P_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HIR3694634.1"
                     /translation="MPNPKRRHSKSRTRQRRAHDALRAPQTVPCPNCQEPRLPHRVCP
                     KCGQYKGREIVR"
BASE COUNT          966 a         1781 c         1984 g          898 t
ORIGIN      
        1 gtaggcgctc gggttctcga cgggcgtgag gacgatggtc gccgccgcgc cgcgcgcttc
       61 gtgctcgcgg atgacggcgc cgaggtcgag gtccgtcagg atgtctccgt tgaagacgac
      121 ggtcggctcg cggatcaggt cttcggcgaa cttgtaggcg ccgtcggtgc ccatcggctg
      181 cggctcgacc gtgtaggcga tcttcacgcc gtagtcggag ccgtcgccga gctgctgctc
      241 gatcttgagc ggctggtagg agagcgagag cgtgatgtcg gtgatgccgg cgcggcgcag
      301 cgtgtcgatc tggtagagca ggaacgggcg gttgcagatg gggacgaccg gcttcggcgt
      361 gtagaccgtc agcggcctca gccgcgtgcc cttgccgccc gcgagtatga gtgcttgcat
      421 gtcgtcgaaa gatgtctggc cgtcagcggg ccgtcgtcga tgattaacgt taaatgaatc
      481 ggtttgcaga ccgtgcgcga gtctagcacc gtcgcggcag ccttttcaat cgccccattt
      541 tcgaggcggt gacgttgctt gacacccctc cgagcgaacg tataatcgaa ctttggtgac
      601 gagaacgatt taacggacat agcagacctt cattaaccat cgagacatca tccgtagtga
      661 ctaagggaga ggccatgccg gagaacaggg aagcggtcat catcagcgcg gcgcggacgc
      721 ccaccgggaa gttccagggc gcgttgaagg acttcacggc gacgcagctc ggcgcgctcg
      781 tcgtgcgcga ggccgtgcgg cgcgcgggcg tcgcgcccga ggacgtggac gaggtcatcc
      841 tgggctgcgt catacaggcg ggactcgggc agaaccctgc gcggcaggcg gcgctgaacg
      901 gcggcatccc gttcggcgtc tcggcggtga cgataaacaa agtgtgcggg tcgggtctga
      961 agtcggtgat gatggcggcg cagggcatcc ggttgggcga ctcggaaacg gtcgtcgcgg
     1021 gcgggatgga gtcgatgtcg aacgcgccgt acctgttgcc gaaggcgcgc gaaggttacc
     1081 ggatgggcaa cggcacgctc gtcgattcga tgatcaacga cgggctgtgg tgcgcgttcg
     1141 agaattacca catgggcaac acgggcgagg tcgtcgccga gcgctacggc gtgacgcgcg
     1201 ccgagcagga cgagtacgcc ttgaactcgc atcgcaaggc cgccgccgcg cagaaggccg
     1261 gcaagttcaa ggacgagatt ctgcccgtcg agattccgca gaagaagggc gcggcgatca
     1321 tcttcgacgc cgacgagtcg gtgcgcgaag acagttcgat ggaagcgctc gggaagctga
     1381 agcctgcgtt taagaaagag ggcgggacgg tgaccgccgg gaacgcgccg ggcgtcaacg
     1441 acggtgcgtc ggcgctggtc gtcacgtcgc tggagcgcgc gcgcgcgctg ggcgtcgagc
     1501 cgatggcgcg catcgcggcg caggccacct cgggcatcca gccggagttg gtgatgatgg
     1561 cgccggtcga ggcgattcgc aaagtcgtca agaaggcggg ctggtcgctg aacgaggtgg
     1621 atttgatcga gctgaacgag gcgttcgcgg tgcaggccgt ggccatcatg cgcgagctgg
     1681 aactcgaccc gcagaaggtg aacgtcaacg gcggcgcggt cgcgctcggc cacgccatcg
     1741 ggcagtccgg cgcgcgcctg ctgacgacga tgctctacga gatgaagcgg cgcgacgcgc
     1801 ggcgcgggct ggccgcgctc tgtctcggcg gcggcaacgc cgtcgcgctg gccgtcgaaa
     1861 gataaaaaga agggtgttgg gtgttaggta ttgggtgttg ggtgaaagac aagcttttat
     1921 ccaacaccta acaccctcag aggtagttat gacccccaca gaccacaaca aggtgctcag
     1981 cattctgcac ctcatctacg gcgggctgaa cttcatctcg ctcctcatcg tcatcccctt
     2041 tttcttgatc ggcattccgt tcctcgggag cggcagcggc gagggggcgg tgttgggggc
     2101 ggtcttcatg ttcacggcgc tcctcatcgg cttcttcgcg ctgctcttca gcctgccgcc
     2161 gctcatcgcc gcctacggca tgcacaagca taggccgtgg gcgcgcaccg cagggatagt
     2221 cgccgccgtc ttcgccgcca tcagcttccc gctcggcacg gcgctgtgcg tctactcctt
     2281 ctggttctac ttcggcaacg aggggcgtgc ctactacgag ggcgcgggcg cgtcgccgca
     2341 ctggcgcggc gcgctcggcg acgcgcccgc gtccacttac ggctgggacg cgcagcgcgc
     2401 ggcgggcgag cgagcctaca cgccgccgcc gccgccgccg agctggcgcg actgaacact
     2461 gcaagcactt cggagaatcg atcggtgtct gaaatcatcg gcgtcatcgg cgcagggacg
     2521 atgggcaacg gcatcgcgca ggtgggggcg cgcgcgggct acgaggtcgt catgcgcgac
     2581 gtggaagacg agttcctgcg gcgcggcctc gcggccatcg accgcagcct gcaacgcgac
     2641 gtggacaagg agcgactgac cgaagaggag aagcgcgccg tcctgggccg catccgcacg
     2701 acgaagaagc tgcaagacct cgcgggcgcg agcttcgtcg tcgaagccat caccgagaac
     2761 ctcgacgcga agcgagatgt cttccgcgcg ctcgacgagt tgatcgggcc ggaggcgatt
     2821 ctcgcgtcga acacttcgtc gatctcgatc acgaaactgg gcgcggcgac gaagaggccg
     2881 gacaaggtca tcggcatgca cttcatgaac cctgtgccgg tgatgaagct ggtcgaggtc
     2941 atccgcggca tcgccacctc ggacgagacc tacgagaaga cgcgggcctt gtgcgagcgg
     3001 atggggaaga cggcgctcga ctgcaacgac tcgccgggct tcgtctcgaa ccgcgtgctg
     3061 atgccgctca tcaacgaggc gatcttcgcg ctccacgagg gcgtcgccac gcgcgagagc
     3121 atcgacggca tcatgaagct cgggatgaac cacccgatgg ggcctctgac gctcgccgac
     3181 ttcatcggcc tcgacgtgtg cctcgccatc atgaacgtgc tgcacgctga gctgggcgac
     3241 ccgaagtaca ggccgtgccc gctgctccgc aagtacgtgg acgcgggctg gctgggccgc
     3301 aagtcgggcc gcggtttcta cgaatactag attagaagtc aggagtcgga agtcggaagt
     3361 cagaatgaag acagccaagc aagttgcgga gttcttcatt ccttcttccg acttctgact
     3421 tccgactcct gacttcctgt tatacttacg ttatgcccgt cggacgtgga cagattttct
     3481 gccagcggtg cctcgcggcc aaccccgtcg agagggattt gtgcgagcgc tgcggcacgc
     3541 ggctgatgct cgtcgtcgag cctgccgcgc accgcttcga ggaagactcg ggcgcgtgcg
     3601 actacgaaga gcacctgctc gaacgcgtca ccgtgctcga acaccacctc acgcgcttcg
     3661 ccgagaagct ggagaagacg ctcgacctct tgctccgtca ggcgcgcagc tcctacatgg
     3721 atcacgcgct ggtcgagtcg ctcatcgcgc tcctggccga ggcgggcacc atcaaccgcc
     3781 agacgctcat caccgtctgg cgcgagatgt gcgagcgcga gaacacgccc gcgctggccg
     3841 ccggaagggc cgacgcggcc cgcgaccgcg tgctcgccca cggggcgggc ggcggcggcg
     3901 tgccggagcg gttcgagcgg ctggtgcgcg aagggttcag gcgcgtgggc gagggcgacc
     3961 gcgcaggggg cgtgcgcgag cttgagaagg cggcggcgct ggcgccgacg aacactccgc
     4021 tgcacgcctt cctcggcgaa tacttcttcg gcgagggcaa gacggcgctg gcgcgcgact
     4081 acctctcgct cgctctggcg gagccggagg cgggcgactg gcgcgtgcgt ctgctgctcg
     4141 gcctcgcctg cggcgacgag ggcgacgcgc gccgcgcgaa ggagctgctg cacgaggcca
     4201 tcgcgcgcgg cggcccgtcc ttcgcggcgc actacgcgct cgggcgcctg ctcgccgccg
     4261 agtccgactg gaagtccgcg ctcgccgagt tcaagcgtgc gctcggcgcg cgcgagtgcc
     4321 ccgaggcgca ctacgtgctc ggcctcgtct actacgaact cgaacgctac ccgacggcgc
     4381 tgcggcacct gacgaaggcc gtcaagacct cgcccgatta cggcgaggcg ttctacctgc
     4441 tcgggctggt gcggctgcgt ctggggcaga aggagcaggc gtgcgaggcg ttcgacgcgg
     4501 cgcaggcgct cgaccgcgag gagccgcgct accgcgccgc gcgccgcgcc gccgggcgca
     4561 ggggggcgct cccgccgccc gcgcccgcgc tcgtctcgac gaaggggcgc gcgcggcgcg
     4621 ggctggtcac gggcggcgac ctcaggctcg ccgccgcgct gcgcgaagac gcgctcggcg
     4681 actcgccgtc gcgctgactt acggcgtgcc gggccgctgc cgcgttgaca ctcgcgccgc
     4741 gaccgctata attgcacctt ttgcgcggaa ggccgcgcgc gacgccgttc gtgaccgtgg
     4801 ggtgtgtgat gcaaatcgaa gtcgagcggc tggacgagcg cggggaagct ttcgcgcaca
     4861 cgtactcgga aaacgagctg agcctcgaag acgagctggc gcggctcgtg tccggcgcga
     4921 gcgtgcaggg gaacgcgacg cgcaagggcg acgaggtcaa gctgcgcggc acgttgagcg
     4981 ccgaggtcga ggtctactgc gaccgctgcg cggcggccat cgtcgccccg ctcgaaatag
     5041 agttcgacac ctcgttcctc ccggcggagg tcgagaaggc cgcggtcgag aacgtcgagc
     5101 ttcagaaaga cgacctcatt cgctcggtct acgagggcgg cgcggtcgat ctcgacgagc
     5161 tggtgcgcga gcaaatcctg ctcgcgctgc cgacgcgcag cctgtgccgc gaagagtgca
     5221 aaggcttgtg ccccgagtgc ggggcgaacc tgaacgcggg cgagtgcgac tgcgcgcgga
     5281 aggaaatcga cccgcgctgg gccgcgctcg ccgacttgaa gaaagacgag taaatggtga
     5341 atggtaaatg gtagatggat aaagcctatc cagacagttg ccttgctatt cacatctcaa
     5401 gctaaagaag cgttagccac catttactat tcaccattca ccatttacta aggagctatc
     5461 atgcctaatc cgaaacgccg ccactcgaaa tcgcgcacgc gccagcgccg cgcgcacgac
     5521 gccctgcggg cgccgcagac cgtcccgtgc ccgaactgcc aggagccgcg gctcccgcac
     5581 cgcgtctgcc cgaagtgcgg ccagtacaag ggccgcgaga tcgtccgcg
//
LOCUS       DBNGOH010000206         2841 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1864633, whole genome shotgun sequence.
ACCESSION   DBNGOH010000206 DBNGOH010000000
VERSION     DBNGOH010000206.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2841)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2841)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2841
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1864633"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(193..1605)
                     /locus_tag="AC159P_03935"
     CDS             complement(193..1605)
                     /locus_tag="AC159P_03935"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3694635.1"
                     /translation="MINSVRGRLTLWYVSALALILITFGASVYVMLSRALHRRVDEAL
                     RSTLDISIISLTHDAQEGQSSQSAAQSTTAELSHPQQAMMIFDADGRLLAEHPYEDDL
                     HVRLPDLGAIPDDDVYLYTVAEEGDADDRHRLAVRRVRIPPTDTPFIILASQPLEAVE
                     DELKSLREILYFATPAALLLAGLGGWFLARQGLAPVASMARSARQIGAGSLDRQLPVA
                     NPRDELGQLATTFNELLSRLDAAFDEQRRFMADASHELRTPLSVMTTAAGVTLKKEHR
                     DEDEYREALRMMGEQTRRLSRIVQDMFTLARADAGRYPLRERTLYLDDLLAEVARVGG
                     MLVSDKNVAIELTNLPEAAFHGDEDLLRQMLLNLVDNAVKFTPPGGAIRLGLERRGEW
                     YRLSVSDTGPGIPAEARAHIFERFYRADKARSRAEDGGAGLGLAIARWIAQAHDGDLE
                     LADSDGAGTTFVARLPLPRP"
     gene            complement(1598..2287)
                     /locus_tag="AC159P_03940"
     CDS             complement(1598..2287)
                     /locus_tag="AC159P_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR3694636.1"
                     /translation="MRILLVEDEPDAAGILAKGLREEAYAVDVAGDGERALELAFVND
                     YDLVILDVMLPRRDGFEVCRELRSNGSAVPVLMLTARDAVEDRIAGLDTGADDYLSKP
                     FDFDELLARARALLRRKPMLLPAVINVSDMAVDTRARRVSRGGRDVELTAKEYALLEY
                     LARRAGEVVSRADIAEHVWDESYDPFSKVIEVFIQRLRRKVDEGHPLKLIRTRRGEGY
                     MLTTEGADADD"
     gene            complement(2291..>2841)
                     /locus_tag="AC159P_03945"
     CDS             complement(2291..>2841)
                     /locus_tag="AC159P_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019342105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HIR3694637.1"
                     /translation="AGRWSGFRESALHLGELLMDIIWQELTSGLPDGRQLVHVLIRLV
                     AATLLGAVVGMQREKAGKPAGLRTHMLVTLGTAVFVLACSGVGMSSDGLSRVIQGIVT
                     GIGFIGAGSILKLNEERDVQGLTTSAGVWMTAAIGVAVGLGSLGVAILSTIFTLFILS
                     FATRFEHRADERRAGDAAAGNK"
BASE COUNT          464 a          950 c          983 g          444 t
ORIGIN      
        1 tcgctgacga taaccgccac gacgcacgcg aagagcacta acatcccgac gaccggcagt
       61 gccatcttca tcctgtagct cataaacttc accccttgca gtacggacct ctcataacag
      121 attcgaccat gaagtataga ggatcgaagg taaacggacg gtaaacgact ggaacgtcaa
      181 gcgggcggtc ggttacgggc gcgggagggg gaggcgggcg acgaaggtcg tgccggcgcc
      241 gtccgagtcg gccagttcga ggtcgccgtc gtgggcctgc gcgatccagc gggcgatggc
      301 gaggccgaga ccggcgccgc cgtcttcggc gcgcgagcgg gccttgtccg cgcggtagaa
      361 gcgttcgaag atgtgggcgc gcgcctcggc agggatgccg gggcctgtgt cggagaccga
      421 gagccgatac cactcgccgc gccgctcgag gccgagccgg atggcgccgc ccggcggcgt
      481 gaacttcacg gcgttgtcca cgaggttcag gagcatctgg cgcaagaggt cttcgtcgcc
      541 gtggaaggcg gcctccggca agttggtcag ctcgatggcc acgttcttgt cggagaccag
      601 catgccgccc acccgcgcca cttccgcgag caggtcgtcg aggtagagcg tccgctcgcg
      661 cagagggtag cggcccgcgt cggcgcgcgc cagcgtgaac atgtcctgca cgatccttga
      721 gagccgacgg gtctgctcgc ccatcatccg cagcgcctca cggtactcgt cctcgtcgcg
      781 gtgctctttc ttcaacgtca cgcccgccgc cgtggtcatc accgacaggg gggtgcgcag
      841 ctcgtgcgag gcgtcggcca tgaaccggcg ctgctcgtcg aaggccgcgt cgagacgcga
      901 gagcagttcg ttgaaggtgg tggcgagctg gcccagctcg tcgcgcgggt tggcgacggg
      961 caactggcgg tcgaggctcc cggccccgat ctggcgcgcg ctccgcgcca tcgaggccac
     1021 gggcgcgaga ccctgacgcg ccaggaacca cccgccgagg ccggcgagga gcagcgccgc
     1081 gggagtggcg aagtagagta tctcgcgcag cgatttcagc tcgtcctcga cggcttcgag
     1141 cggctggctg gcgaggataa tgaagggcgt gtcggtcgga ggtatccgca ccctgcgcac
     1201 ggcgaggcgg tgccggtcgt cggcgtcgcc ctcttcggcc acggtgtaga ggtaaacgtc
     1261 gtcgtcgggg atcgcgccga ggtcgggcag gcggacgtgc aggtcgtctt cgtaagggtg
     1321 ctcggcgagc aggcgcccgt cggcgtcgaa gatcatcatc gcctgctgcg ggtgcgacag
     1381 ctcggcggtg gtgctctgcg cggcgctctg gctggactgc ccttcctgcg cgtcgtgcgt
     1441 gagcgagatg atcgagatat cgagggtcga gcgcagggcc tcgtccacgc gccggtggag
     1501 cgcgcgcgag agcatgacgt agaccgaggc gccgaacgtg atgaggatca gcgcgagcgc
     1561 gctcacgtac cagagagtca gccggcccct gaccgagtta atcatcggcg tccgctcctt
     1621 cggtcgtcag catgtagccc tcgccgcgcc gcgtgcggat gagcttcagc gggtggccct
     1681 cgtccacctt ccggcgcagg cgttggatga agacctcgat gaccttcgag aaggggtcgt
     1741 aggattcgtc ccacacgtgc tcggcgatgt ccgcgcggga gacgacctcg cccgcgcgcc
     1801 gcgcgaggta ttcgagcagc gcgtactctt tggccgtcag ttccacgtcg cggcccccgc
     1861 gcgagacgcg ccgcgcccgc gtatcgacgg ccatgtccga gacgttgatg acggcgggga
     1921 gcagcatggg cttgcggcgc aggagcgcgc gcgcgcgcgc gagcagctcg tcgaagtcga
     1981 agggcttcga cagatagtcg tcggcccccg tgtcgagtcc ggcgatgcgg tcttcgacgg
     2041 cgtcgcgggc cgtgagcatc aggacgggca cggctgagcc gttcgaccgc agctcgcggc
     2101 agacctcgaa gccgtcccgg cgcggcagca tcacgtccag aatgacgagg tcgtagtcgt
     2161 tgacgaacgc cagctccagc gccctctcgc cgtcgcccgc cacgtccacg gcgtaggcct
     2221 cctcgcgcag gcccttggcg agtattccgg cggcgtccgg ctcgtcctcg accagtaaga
     2281 tacgcatgtt ctatttgttt ccggcggcgg catcccccgc ccgtctttcg tcggcacgat
     2341 gttcgaaccg cgtcgcgaac gagaggatga agagcgtgaa tatagtgctg agaatcgcca
     2401 cccccagact gcccagcccg acggccaccc cgatggccgc cgtcatccag acgccggctg
     2461 acgtggtcaa cccctgtacg tcgcgttcct cattcagctt gaggatagag ccggccccga
     2521 taaagccgat gccggtgacg atgccctgta tgacgcgcga caggccgtcc gaagacatgc
     2581 cgacgccgga gcacgccagg acgaagacgg ctgtcccgag cgtgacgagc atgtgcgtcc
     2641 gcagccccgc cggcttgccc gccttctccc gctgcatccc cacgaccgcg ccgagcagcg
     2701 tggcggccac cagccggata agcacgtgca ccaactgcct gccgtcaggc agccccgagg
     2761 tcaactcctg ccagatgatg tccattaaca actccccaag atgtaacgcc gactcgcgga
     2821 agccactcca gcgcccggcg a
//
LOCUS       DBNGOH010000207         1556 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_947942, whole genome shotgun sequence.
ACCESSION   DBNGOH010000207 DBNGOH010000000
VERSION     DBNGOH010000207.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1556)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1556
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_947942"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..934
                     /locus_tag="AC159P_03950"
     CDS             <1..934
                     /locus_tag="AC159P_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014396896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flavin-containing monooxygenase"
                     /protein_id="HIR3694638.1"
                     /translation="TAVTLVPAMAERAAHVTMLQRSPTYVVSRPAEDKIANWLRRHLS
                     AHAAYALTRWKNVLLGPFFYNLARKRPELFKRMVAKGVRRQLGEEYASEHFTPRYNPW
                     DQRLCLVPDSDLFRAIREGRASVVTDYIETFTEDGLLLKSGERLDADIIVAATGLVLK
                     LMSGLQLVVDGAPVDLSKRLVYKGMMFSEVPNLAFAIGYTNASWTLKCDLTAEYVCRL
                     LNHMDGHGYAVCTPRANDPDVKEEPVIDFTSGYVRRALHTLPRQGSKRPWRLHQNYAK
                     DLSMMRYGRVEDGTMEFKKAPAARRAGAEAAGRG"
     gene            939..>1556
                     /locus_tag="AC159P_03955"
     CDS             939..>1556
                     /locus_tag="AC159P_03955"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3694639.1"
                     /translation="MRLEGRTAVITGAAGGIGRAIAVSLARRGCHLALADIDEAGMAD
                     TADLVRGDGVRVSRHRLDVADRAAVADLPAQVAAEHAGADLLVNNAGVAVGGTFEQVS
                     EEDFEWLFEINFWGVVRMTRAFLPLLRASGDARVVNLSSVFGLVAPPEQVAYAASKFA
                     VRGFSEALRHELEGSGVGVTVVHPGGVATSIAEKARVPAGVSEEEV"
BASE COUNT          238 a          523 c          559 g          236 t
ORIGIN      
        1 gacggcggtc acgctcgtcc ccgcgatggc cgagcgcgcg gcgcacgtca cgatgctcca
       61 gcgctcgccg acctacgtcg tctcgcgccc cgccgaggac aagattgcga actggctccg
      121 ccgccacctt tcggcccacg ccgcgtacgc gctgacgcgc tggaagaacg tgctgctcgg
      181 accgttcttc tacaacctcg cgcggaagag gcccgagctt ttcaagcgga tggtggcgaa
      241 gggggtgcgc cgccagctcg gggaggaata cgcctccgag catttcacgc cgcggtacaa
      301 cccttgggat cagcgcctgt gcctcgtccc ggactcggac ctcttccgcg ccattcgcga
      361 gggccgcgcc tcggtcgtca ccgattacat cgagaccttc accgaagacg gcctgttgct
      421 caagtccggc gagcgactcg acgccgacat catcgtggcg gccacggggc tggtgctgaa
      481 gctgatgagc gggctgcaac tcgtcgtgga cggcgcgccc gttgacctct cgaagaggct
      541 cgtctacaag gggatgatgt tcagcgaagt ccccaacctc gccttcgcca tcggctacac
      601 gaacgcgtct tggacgctga agtgcgacct cacagccgag tacgtgtgca ggctcctgaa
      661 ccacatggac ggccacggct acgccgtgtg cacaccgcgc gcgaacgacc ccgatgtgaa
      721 ggaggagccg gtcatcgact tcacctcggg gtacgtccgg cgcgccctgc acacgctccc
      781 gcgtcagggg tcgaagaggc cgtggcggct gcaccagaac tacgcgaaag acctttcgat
      841 gatgcgttac gggcgcgtgg aggacggcac gatggagttc aagaaggcgc ctgcggcgcg
      901 gcgcgccggt gcggaggcgg ctggccgtgg ctgagcgcat gcgcctcgaa ggccggacgg
      961 cggtcatcac cggcgcggcg ggcggcatcg ggcgtgccat cgccgtgtcg ctggcgcgca
     1021 ggggctgcca cctcgcgctg gccgacatcg acgaggcggg gatggccgac acggcagacc
     1081 tcgtgcgagg tgacggcgtc cgcgtgtctc gccaccggct ggatgtcgcc gaccgcgcgg
     1141 cggtggccga cctcccggcg caagtcgcgg cggagcacgc gggcgccgac ctgctcgtca
     1201 acaacgcggg cgtggccgtg ggcggcacct tcgagcaggt ctccgaggaa gatttcgagt
     1261 ggctcttcga gatcaacttc tggggcgtgg tgcgcatgac gcgcgccttc ctgccgctgc
     1321 tgcgcgcgag cggcgacgcg cgcgtggtga acctgtcgag cgtgttcggc ctggtcgcgc
     1381 cgccggagca ggtagcgtac gcggcgagca agttcgcggt gcgcggattc tccgaggcgt
     1441 tgcgccacga gctggaaggc tcgggcgtcg gcgtcacggt cgtgcacccc ggcggcgtcg
     1501 ccacctctat cgccgagaag gcgcgcgtcc cggcgggcgt gagcgaagag gaggta
//
LOCUS       DBNGOH010000208         9930 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2597027, whole genome shotgun sequence.
ACCESSION   DBNGOH010000208 DBNGOH010000000
VERSION     DBNGOH010000208.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 9930)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9930)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9930
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2597027"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..447)
                     /locus_tag="AC159P_03960"
     CDS             complement(<1..447)
                     /locus_tag="AC159P_03960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694640.1"
                     /translation="MLHHKAYSIGKLLAGALLCAAASPVFGQQQQPPSSPTYTPAPSA
                     NTPQQGTTPPAQSPQTASPTQAQTQQSANPAAPASPQPGQTTTGAAFSGTLLGTPQTE
                     GQTGQPAQQGQPPQTGAPASEVQPPPGVRPPGRESVPAAAPDDLGLD"
     gene            complement(474..980)
                     /locus_tag="AC159P_03965"
     CDS             complement(474..980)
                     /locus_tag="AC159P_03965"
                     /inference="COORDINATES: protein motif:HMM:NF024953.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HIR3694641.1"
                     /translation="MTLLVLLALLVVPYLLLTAAGRGVAALRVAPPVRARVGLTLFFA
                     FTALGHFVRGEEMSAMLPAAVPYRLGIVYLTGALELLGAAGVWVPGLTRVTGACLILM
                     LLCFLPANIYSALYRVDFGGHGDGPAYLILRVPFQLFVIWWTAFATEQKWLSRRAERS
                     AESVQGVR"
     gene            complement(1000..4386)
                     /locus_tag="AC159P_03970"
     CDS             complement(1000..4386)
                     /locus_tag="AC159P_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016483779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR3694642.1"
                     /translation="MQKLAEICVRRPVFATMLVLSLTVVGAFSFMTLGVDLFPKIDLP
                     TITVTVVNPGASPREIETEITDKVEAAVNTISGIDELRSTSVEGVSQVFITFLLDKNA
                     ETAANEVRDKVNLITGDLPETAEQPIIQKLDTDAAPVLRIAVSAPRSLRDVTDIADKQ
                     IKKRIESINGVGNVEIVGGAEREIHVWVDPDKMRAFNLTVPEVVAAIRAQNLEVPGGR
                     VDEGTRELTVRTMGRITDPVDFNTVSVGLRGGYPVLVKDIGYVEDSTVEQRTAARLNG
                     QPAVTLIVAKQSGQNTVSVADAIKERLAEIAPTLPKDVRTQIVGDQSIFIKASVEAIE
                     THLVEGGLLAALVVFLFLWNVRSTFIAAIAIPTSIIATFGLMAAMGYTLNQITMLALT
                     LMVGIVIDDAIVVLENIYRFIEEKGMPPFQAAIEGTREIGLAVMATTLSLLAVFIPVG
                     FMQGIVGRFMSSFGLTSAFAVAVSLVVSFTLTPMLAARLIKRKEAPEGPGTASPPSEG
                     DAPALLADESRVVAGEPAKHAEGGIIDERMGLWMSDAEQEAGKDVPPQAAAEADRPPA
                     PGPRPLESHADSKDSRFYRPIDRTYTWLLRWSMAHRWVVVLMCVAVIISIVPLFSFVG
                     KNFLPVDDQSQFEVNVRAPEGATLAATSALVERIAADIRQSVPGVTDTLVTIGGGQQE
                     QVNVASIYVKLAPIEEREEMQDELMVAARNLLGKYPQDLRTSVQQVAAISGGGFRNAD
                     IQYVIGGPDLQKLTEYSDRLLEKMKTIPDVVDVDSSLVNGKPEVRVVIDRARAADMGV
                     RVGDIAQALNVLVAGQEVSTFNEGTDQYDVRVRAVGTARTSPQALENLFVQSSKTGWT
                     NLSNLVRVEEGTGPSAIERLNRQRQVTLTANVRPGGSQSAVIEQLNAYAAELNIDPAY
                     STGLAGRSKELGKAGGYFLLAFALSFIFMYMVLAAQFESFIHPVTILLTLPLAIPFGI
                     LSLLVTGQTVNIFSGLGLLLLFGVVKKNAILQIDHTNELRAHGMERMEAIIQANRDRL
                     RPILMTTIALVAGMLPLTISSGPGSGTNRSIGVLVVGGQSLCLLLTLLAVPVFYSLFD
                     DVSRARVFSRTASRVGGAFAWARRRAATAAGSWLGTFGRH"
     gene            complement(4485..5750)
                     /locus_tag="AC159P_03975"
     CDS             complement(4485..5750)
                     /locus_tag="AC159P_03975"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR3694643.1"
                     /translation="MVSRHHSKTVALFLALVSLAAFNASCGGRTAQANKKAEEQAAAT
                     PPPAVEVTTAPAITRQLPRFIEATGGLAADEQTDVAPTVGGRVVAVGVDLGSYVQRGA
                     VLVRLDDADARLRVEQAEAQLAQAQAAVRQAEERIGLRPGQQFDPLRVAEVGAARAAL
                     DLAERQLRRYEQLLESGDVSRSAYDQQKAQRDQLRQQYEAQVALARQSFAGVQTARAA
                     ASTARVAVEQAQKSVRDVVVYAPISGYVADRPADLGEYISTSMKVATIVRTNPLRVRI
                     DIPEQSISSISTGQSVSVSVSGYPDRSFAGRVARISPNVSAESRTLTVEAEVENGGNL
                     LKPGQFATIRILLPESTPATLVPARAVRTEGSTSRVYVIKDGVAQERLVQLGQQDGDL
                     VEIKGNVAADEPVATSNVEQLGDGVPVRQ"
     gene            complement(5756..6547)
                     /locus_tag="AC159P_03980"
     CDS             complement(5756..6547)
                     /locus_tag="AC159P_03980"
                     /inference="COORDINATES: protein motif:HMM:NF012655.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR3694644.1"
                     /translation="MTRQQESARAKEPAPPPAPTGVRMAGDERRRQIVRVAMRLFSER
                     GFRGTTTKEIAQAAGVSEAIIFRHFATKDELYSAIIDHKGCAEQSGEWANQPVAELIR
                     FEVGDALARKDDRAVFEGIALSMLRHQQEEPEFLRLLLYSALEGHQLAQMFWERNVRA
                     LYEFLGSYIRERQRDGVFGEVDPHVVVRAFSGTLIHHTLSNTLWDKSRSLLSISDEEA
                     ARQFTEILLNGVMNARAQGAAKHAPSRTKSTKRAASTSRGNNKKK"
     gene            6937..7335
                     /locus_tag="AC159P_03985"
     CDS             6937..7335
                     /locus_tag="AC159P_03985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ElaB/YgaM/YqjD family protein"
                     /protein_id="HIR3694645.1"
                     /translation="MSQNFNDNDTPNTTGGTGFGGTSDASTGLSTTSTGTGGGSAAGT
                     ATAPSTSGIASQAKEYGQKVADAATKAKDYLSDRASQAGEKIQDLRNVDYGQLAEDAK
                     DYARQKPGQAILIAAAAGFVVGLLLRGSRR"
     gene            7440..7922
                     /locus_tag="AC159P_03990"
     CDS             7440..7922
                     /locus_tag="AC159P_03990"
                     /inference="COORDINATES: protein motif:HMM:NF018980.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HIR3694646.1"
                     /translation="MADLERRTAAAPAPAREPLQADIDSLPALFGRLGDNVMQLVDTK
                     LSLIKVELKEDASFYARNSALLAVGAVVATIGFALVNVAVAFFIASFFGSDAAGAVVE
                     RYTPTSYGLGFLITGVVYLVIGGALILVMKNRLAAHNPVPVRTVEEVRKDKQWLKNEI
                     "
     gene            7901..8782
                     /locus_tag="AC159P_03995"
     CDS             7901..8782
                     /locus_tag="AC159P_03995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694647.1"
                     /translation="MAEERNLSLARTTVDEADEEATDATKAELQRRMDEARESITHTV
                     TEIKETVMNQYQQVRESISDSLDWREQYRRRPVPFTVGAFGVGLVLGYSIANAVKGDG
                     AADDDDYDTDEAFDRIEQGFDRMQSQPVRAYAAQAITGAAGHALAAPAPRADVGPETR
                     PAYAGGGYDAPASTTTAEEEEPQGPGLMARFKETRAYDRLQSELSTLGDRAIDELSKT
                     AQTVVVPALLAKLKDLIGIDLSTQREVAQRGRLEHDTARAGAATAAAAEGQTPAQGAG
                     TGTGGAASAQGAGGARS"
     gene            8967..>9930
                     /locus_tag="AC159P_04000"
     CDS             8967..>9930
                     /locus_tag="AC159P_04000"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007881482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol/lipid kinase family protein"
                     /protein_id="HIR3694648.1"
                     /translation="MHVASQESQVRQAETGPPGAPHVEVILNAAAGADGGDDLREMVA
                     DAFAACGVEARVCAARDGGEAFELARRAVEDGARVVVAGGGDGTVNAVASLLVGTDCA
                     LGVLPLGTLNHFAKDLGIPLDLWAAARVVCRGRVARVDAGEVNGRVFVNNSSLGLYPR
                     IVRRRRRMQERLGSSKWAAFFWAALAVLRRNPFLRVRLQADGREIVRETPFVFVGNNE
                     YEIESWQIGARTRLDAGTLSLYVTHRKGRLGLAWLAARALLGRLREAKDFDALRVSEV
                     VVETHRAGKRLPVAADGEVALMKTPLHYRALPGALRVIVPEEVKT"
BASE COUNT         1558 a         3257 c         3535 g         1580 t
ORIGIN      
        1 gtcgaggccg aggtcgtcgg gcgccgccgc gggcacgctc tcgcggcccg gcgggcgcac
       61 gcccggcggc ggctgaacct cggacgcggg cgcgccggtc tgcggcggtt gaccttgttg
      121 cgccggctga cccgtctggc cttcggtctg cggcgtgccg agcagcgtgc cggagaaggc
      181 ggcgcccgtc gtcgtctggc cgggttgcgg cgaggcgggc gcggcggggt tggccgactg
      241 ctgcgtctgc gcctgcgtgg gcgaggccgt ctgcggcgac tgcgcgggcg gcgtcgtgcc
      301 ctgctgcggc gtgttggccg aaggcgcggg cgtgtacgtc ggactcgacg gcggctgctg
      361 ctgttgcccg aagacgggcg acgcggcggc gcacagcagc gcgcccgcga ggagcttgcc
      421 gattgaataa gccttgtgat gaagcatgac cttgatgttc tttacgaatg ttcctaacga
      481 acgccctgta cgctctccgc cgaccgctcc gcccggcgcg agagccactt ctgttcggtg
      541 gcgaaggccg tccaccagat gacgaagagt tggaacggca cgcgcaaaat cagatacgcg
      601 gggccgtcgc cgtggccgcc gaagtccacg cggtagagcg ccgagtaaat gttggcgggc
      661 aggaagcaga gcagcatgag aatcaggcac gcgcccgtca ccctcgtcag gccgggaacc
      721 cagacgcctg ccgcgcccag gagttcgagc gcgcccgtca ggtagacgat gccgagccgg
      781 tacggcaccg cggcgggcag catcgcggac atctcttcgc cgcggacgaa gtggccgagc
      841 gccgtgaagg cgaaaaagag cgtgagtccc acccgcgcgc gcaccggcgg cgcgaccctg
      901 agcgcagcga cgccgcgccc cgctgcggtg agcagcaggt acgggacgac gagcagtgcg
      961 agtaggacga gaagcgtcat gtcggttgcg gatttcggat tagtgtctcc cgaaggtgcc
     1021 gagccacgag ccggcggcgg tggcggcgcg acgccgcgcc caggcgaagg cgccgccgac
     1081 gcgcgaggcc gtgcggctga agacgcgcgc gcggctcacg tcgtcgaaga gcgagtagaa
     1141 gacgggcacg gccaggagcg tcaggagcag gcagagcgac tggccgccga cgacgaggac
     1201 gccgatggag cggttggtgc ccgaacccgg ccccgaagag atcgtcagcg ggagcatgcc
     1261 cgcgacgagc gcgatggtcg tcatcaggat gggccggagg cggtcgcggt tcgcctggat
     1321 gatggcctcc atccgctcca tgccgtgcgc gcgcagctcg ttcgtgtggt cgatctgcaa
     1381 gatggcgttc ttcttcacga cgccgaagag caggagcagg ccgaggccgg agaagatgtt
     1441 cacggtctgg cccgtcacca gcaagctcag gatgccgaac gggatggcga gcggcagcgt
     1501 caggagaatc gtgacggggt ggatgaacga ttcgaactgc gccgcgagca ccatgtacat
     1561 gaagatgaac gagagcgcga aggcgagcag gaagtagccg cccgccttgc ccagctcctt
     1621 cgagcgaccg gcgaggccgg tggagtaggc cgggtcgatg ttcagctcgg cggcgtaggc
     1681 gttcagttgt tcgatgaccg ccgactgcga gccgcccggc ctgacgttgg ccgtgagcgt
     1741 cacctgccgc tgccggttaa ggcgctcgat ggccgacggc cccgtcccct cctcgacgcg
     1801 gacgaggttc gacaagttag tccagccggt cttcgaagac tggacgaaca gattctccaa
     1861 agcctgcggg ctggtgcgcg ccgtgcccac cgcgcgcacg cgcacgtcgt actgatccgt
     1921 gccttcgttg aaggtcgaga cctcctgccc tgcgacgagg acgttcagag cctgcgcgat
     1981 gtcgccgacg cgcacgccca tgtcggcggc gcgcgcgcgg tcgatcacga cgcgcacctc
     2041 gggcttgccg ttgacgagcg aagagtcaac gtccaccacg tccgggatgg tcttcatctt
     2101 ttcgagcagg cggtcggaat actccgtcag cttctgcaag tcggggccgc cgatgacgta
     2161 ctggatgtcg gcgttgcgga agccgccgcc ggagatggcc gccacctgct ggacgctggt
     2221 gcgcaaatcc tgcgggtact tgccgagcag attgcgcgcc gccaccatca gctcgtcctg
     2281 catctcttcg cgctcctcaa tcggcgcgag cttgacgtag atcgaggcga cgttgacctg
     2341 ctcctgctgc ccgccgccga tggtgacgag cgtgtcggtg acgccgggga cgctctgccg
     2401 tatgtcggcg gcgattcttt cgacgagcgc ggaggtggcg gcgagcgtcg cgccttcggg
     2461 cgcgcgcacg ttgacctcga actgcgactg gtcgtccacc ggcaggaagt ttttgccgac
     2521 gaagctgaag agcgggacga tgctgatgat gaccgcgacg cacatcagca cgaccaccca
     2581 gcggtgcgcc atcgaccagc ggagcagcca cgtgtaggtg cggtcgatgg gacggtagaa
     2641 gcgcgagtcc ttcgaatcgg cgtgcgattc gaggggccgg gggccggggg ccgggggccg
     2701 gtcggcttcg gcggctgcct gcggcggcac gtctttgccg gcctcctgct cggcgtcgga
     2761 catccagagg cccatccgct cgtcgatgat gccgccttcg gcgtgcttcg ccggctcgcc
     2821 cgcgacgacg cgcgattcgt ccgcgaggag cgcgggcgcg tccccttcgc tcggcgggct
     2881 ggccgtgccc ggcccttcgg gcgcttcttt gcgcttgatg agacgtgcgg cgagcatcgg
     2941 cgtcagcgtg aacgagacga cgagcgagac ggcgaccgcg aaggcgctcg tcagaccgaa
     3001 cgaggacatg aagcgcccga cgatgccctg catgaagccg accgggatga agacggcgag
     3061 caagctgagc gtcgtcgcca tgacggccag accgatctcg cgcgtgccct cgatggccgc
     3121 ctggaagggc ggcatcccct tctcttcgat gaagcggtag atgttctcca agacgacgat
     3181 ggcgtcgtcg atgacgatgc cgaccatcaa ggtgagcgcg agcatcgtga tctggttcag
     3241 ggtgtagccc atcgccgcca tcaggccgaa ggtggcgatg atcgaggtcg ggatggcgat
     3301 ggccgcgatg aacgtcgagc gcacgttcca gaggaagagg aagacgacga gcgccgccag
     3361 caggccgccc tcgacgaggt gcgtctcgat ggcctcgacc gacgccttga tgaagatcga
     3421 ctggtcgccg acgatctgcg tccgcacgtc tttcggcagc gtcggcgcga tctcggccag
     3481 ccgctccttg atcgcgtccg ccacgctcac ggtgttctgc cccgactgct tggcgacgat
     3541 gagcgtcacg gcgggctggc cgttgaggcg cgcggcggtg cgctgctcga ccgtgctgtc
     3601 ctcgacgtag ccgatgtctt tgacgaggac ggggtagccg ccgcgcaggc ccaccgagac
     3661 ggtgttgaag tccacggggt ccgtgatgcg gcccatcgtc ctgacggtca gctcgcgcgt
     3721 gccctcgtcc acgcgcccgc ccggcacctc caggttctgc gccctgatgg ccgcgaccac
     3781 ctcgggcacc gtgaggttga aggcgcgcat cttgtccggg tcaacccaga cgtgtatctc
     3841 gcgctcggcg ccgccgacga tctcgacgtt gccgacgccg ttgatcgact cgatgcgctt
     3901 cttgatctgc ttgtcggcga tgtcggtcac gtcgcgcaaa ctacgcggcg cgctgaccgc
     3961 gatgcgcagg acgggcgcgg cgtccgtgtc gagcttctgg atgatcggct gctcggcggt
     4021 ctccggcaga tcgcccgtga tgaggttgac cttgtcgcgc acctcgttgg cggcggtctc
     4081 ggcgttcttg tcgaggagga acgtgatgaa gacctgcgag acgccttcga ccgaggtcga
     4141 gcgcagctcg tcgatgccgc tgatggtgtt gacggcggcc tcgaccttgt cggtgatctc
     4201 ggtctcgatc tcgcgggggc tggcgccggg gttgacgacc gtcacggtga tcgtcggcaa
     4261 atcgatcttc gggaacagat cgacgccgag cgtcatgaac gagaacgcgc cgacgaccgt
     4321 caaagacagc accagcatcg tcgcgaagac ggggcggcgg acacagattt cggctaactt
     4381 ctgcatggaa caagctccgc ttgtctaaga gcgattagcg actagctgtc agccgttagc
     4441 tttttgtctt gagctaacag ctgacagcta atcgctaaca gcttttactg cctgaccggc
     4501 acgccgtcgc cgagctgctc gacgttcgag gtggcgacgg gttcgtccgc cgcgacgttg
     4561 cctttgatct cgacgaggtc gccgtcctgc tggccgagct ggacgaggcg ttcctgcgcc
     4621 actccgtcct tgatgacgta gacgcggctc gtgctccctt cggtgcgcac cgcgcgcgcc
     4681 gggacgagcg tcgcgggcgt ggactcgggg agcaggatgc ggatggtggc gaactggccg
     4741 ggcttgagga ggttgccgcc gttctcgacc tcggcctcga cggtgagcgt gcgcgactca
     4801 gccgagacgt tcggcgagat gcgcgcgacg cgaccggcga aggagcggtc ggggtagccg
     4861 ctgacgctga ccgacacgct ctgcccggtc gagatgcttg agatgctctg ctccgggatg
     4921 tcgatcctca cgcgcagggg gttcgtgcgg acgatggtcg ccaccttcat cgaggtcgag
     4981 atgtactcgc cgaggtccgc ggggcggtcg gcgacgtagc cgctgatggg cgcgtagacc
     5041 accacgtcgc ggacggactt ctgcgcctgc tcgacggcga cgcgcgcggt cgaggcggcg
     5101 gcgcgcgccg tctgcacgcc cgcgaacgac tggcgcgcga gcgcgacctg cgcctcgtac
     5161 tgctggcgga gctggtcgcg ctgcgccttc tgctggtcgt aggccgagcg cgacacgtcg
     5221 cccgattcga gcagttgctc gtagcggcgg agctgccgct ccgcgaggtc gagggcagcg
     5281 cgggccgcgc cgacctcggc gacgcgcagc gggtcgaact gctggccggg cctgaggccg
     5341 atgcgctctt cggcctgacg cacggcggcc tgcgcctgcg cgagctgcgc ctcggcctgc
     5401 tcgacgcgca gccgcgcgtc cgcgtcgtcg agacggacga gcaccgcgcc gcgctggacg
     5461 tagctgccga gatcgacgcc gacggcgacg acgcgaccgc ccaccgtggg cgccacgtcc
     5521 gtctgctcgt cggcggcgag gccgcccgtc gcctcgatga agcgcgggag ctgccgcgtg
     5581 atggcgggtg cggtcgtcac ctccaccgcc ggcgggggcg tggccgcggc ctgctcctcg
     5641 gctttcttgt tggcctgcgc cgtgcgcccg ccgcacgacg cgttgaacgc ggcgagcgag
     5701 acgagtgcga gaaagagtgc gacggttttc gaatgatgac gggagaccat gagtcttatt
     5761 tcttcttatt attaccccgg ctcgtgctgg ccgcgcgctt ggtggacttc gtgcgcgacg
     5821 gcgcgtgctt cgcggcgccc tgcgcgcgtg cgttcatgac gccgttcaga agtatctcgg
     5881 tgaactgccg cgccgcctcc tcgtccgaga tggagagcag gctgcgcgac ttgtcccaga
     5941 gcgtgttgct caaggtgtgg tggatgagcg tgccgctgaa ggcgcgcacg acgacgtgcg
     6001 gatcgacctc gccgaagacg ccgtcgcgct gccgctcgcg gatgtacgag ccgaggaact
     6061 cgtagagcgc gcgcacgttc cgctcccaga acatctgcgc gagctggtgg ccttcgagcg
     6121 ccgagtagag cagcaggcgc aggaactccg gctcctcctg ctggtgtcgc agcatggaga
     6181 gcgcgatgcc ctcgaagacg gcgcggtcgt ccttgcgcgc cagcgcgtcg ccgacctcga
     6241 agcggatcag ctcggccacg ggctggttcg cccactcgcc cgactgctcg gcgcagccct
     6301 tgtggtcgat gatggcgctg tagagttcat ccttcgtggc gaagtggcgg aagatgatgg
     6361 cctcggagac gcccgcggcc tgcgcgatct ctttggtcgt cgtgccgcgg aagccgcgct
     6421 ccgaaaagag gcgcatcgcc acacggacga tctgccggcg gcgctcgtcg cccgccatgc
     6481 ggacgccggt cggggcgggg ggcggggccg gctctttggc gcgcgcggat tcctgttgtc
     6541 tggtcaaagt ctcaagctct ccggaacgtg acgagggatt aaaagtaagt acttacttac
     6601 ctcagtacgg gcgggctgtc aagttcgttc ccgaaaattt ccggcggcga cgattttggc
     6661 cgagggcgac gttttcggct ctgtattgtt cccgaccttg tgctaaatta ggcgtacctt
     6721 ttcgcaacaa ataacagagg cttgagagaa ggagcgctgc cacgggttcg gtatcgggcg
     6781 gcgtatccct gacgggcttt ttccctcgct tgtgatgggg caggctttac cggcacgttg
     6841 tttgctcgtt cacggcgggt aaagcagtta tcggggcgaa gactcgcgcg cgccctcgct
     6901 tgataagaga aattcacatt cgaaggagag aaactgatgt cacagaactt caacgacaac
     6961 gacacgccga acaccacggg cggcaccggc ttcggcggca cgtccgacgc ctcgaccgga
     7021 ctctcgacca cctcgaccgg caccggcggc ggctcggcgg cgggcacggc cacggcgccc
     7081 tccacgagcg gcatcgcctc gcaggccaag gagtacgggc agaaggtggc ggacgcggcc
     7141 acgaaggcga aggactacct gagcgaccgc gcctcgcagg cgggcgagaa gattcaagac
     7201 ctgcgcaacg tcgattacgg gcagctcgcc gaggacgcca aggactacgc gcggcagaag
     7261 ccgggccagg cgatcctgat cgccgccgcc gccggcttcg tcgtcggcct cctgctgcgc
     7321 gggtcgcgcc gctgaaagaa acgatgaatg cagagtgcag aatgatgaat aaaagacagg
     7381 gtcttcttta ttcatcgttc tgcgttctga cttctgcctt ttccgagagg gggtgagcga
     7441 tggccgattt ggagagaagg acggctgccg cgccggcgcc cgcgcgcgag ccattgcagg
     7501 cggacatcga cagcctgccc gcgctgttcg gtcgcctcgg cgacaacgtg atgcagctcg
     7561 tcgatacgaa gctcagcctg atcaaggtcg agctgaaaga ggatgcctcg ttctacgcgc
     7621 gcaacagcgc gctgctggcg gtcggcgcgg tggtggcgac catcggcttc gcgctcgtca
     7681 acgtggcggt ggcgttcttc atcgcctcgt ttttcgggtc ggacgcggcg ggcgcggtcg
     7741 tcgaacgtta cacgccgacg agctatggcc tcggttttct catcacgggt gtggtctacc
     7801 tcgtcatcgg cggcgccctc atcctcgtca tgaagaaccg cctcgccgcg cacaaccccg
     7861 tccccgtgag gacggtcgag gaggtgagaa aggataaaca atggctgaag aacgaaatct
     7921 aagtctggcg cgcacgaccg tggacgaggc ggacgaggag gcgaccgacg cgaccaaggc
     7981 cgagcttcag cgccggatgg acgaggcgcg cgagtccatc acgcacaccg tcacagagat
     8041 caaggaaacc gtcatgaacc agtaccagca ggtgagagag tcgatctcgg attcgctcga
     8101 ctggcgcgag cagtaccggc gccgcccagt ccccttcacc gtcggcgcct tcggcgtcgg
     8161 cctcgtcctc ggctacagca tcgccaacgc cgtcaagggc gacggcgccg cggacgacga
     8221 cgactacgac acggacgaag cgttcgaccg catcgaacag ggcttcgacc gcatgcagtc
     8281 gcagcccgta cgcgcctacg ccgcgcaggc catcacgggc gccgccggcc acgcactggc
     8341 cgccccggcc ccgcgcgcgg atgtcgggcc ggagacgagg cccgcgtacg ccggcggcgg
     8401 ctacgatgcg ccggcttcga cgacaaccgc cgaggaggag gagccgcaag gccccggcct
     8461 catggcgcgc ttcaaagaga cgcgcgccta cgaccgcctc cagagcgagc tttcgacgct
     8521 cggcgaccgc gccatcgacg agttgtcgaa gacggcgcag acggtcgtcg tcccggcgct
     8581 cctcgccaag ctcaaagacc tgatcggcat cgatctttcg acgcagcgcg aggtcgcgca
     8641 gcgcggccgc ctcgaacacg atacggcacg cgcgggcgcg gccactgccg ccgccgccga
     8701 aggtcaaacg ccggcacagg gcgcgggcac cggcacgggc ggcgccgcgt cggcgcaggg
     8761 cgcgggcggc gcgcgcagct agtcgcttcc accgtcgccg cgcgcccgcc cgcgcccaca
     8821 cgggcgggcg gcgctcggtg cggtaacagg tcggggtgcg cctcaacggc gacaaccaca
     8881 agggcgagcc tccacgcacg ttggctcgcc cgttttatta tgaggcgtga ctcgcttacg
     8941 cttcgccgcg cgcccctcga actttaatgc acgtcgcttc acaggagtca caggttcggc
     9001 aagccgagac gggtccgccc ggcgccccgc acgtcgaggt catcctcaac gcggcggcgg
     9061 gcgcggacgg cggcgacgac ttgcgcgaga tggtggcgga tgccttcgcg gcgtgcggcg
     9121 tcgaagcgcg cgtctgtgcg gcgcgcgacg ggggcgaggc gttcgagctg gcgcggcgcg
     9181 cggtcgagga cggggcgcgc gtcgtcgtcg cgggcggcgg cgacggcacg gtcaacgcgg
     9241 tcgcttccct cctcgtcggc accgactgcg cgctcggcgt gctgccgctc ggcacgctca
     9301 accacttcgc gaaagacctc ggaatcccgc tcgacctctg ggcggcggcg cgcgtcgtct
     9361 gccgcgggcg cgtcgcgcgc gtggacgccg gcgaggtcaa cggtcgcgtc ttcgtcaaca
     9421 attcgagcct cggcctctac ccgcgcatcg tgcggcggcg ccgacgcatg caggagcggc
     9481 tgggcagctc gaagtgggcg gccttcttct gggcggcgct cgccgtcttg cgccgcaacc
     9541 ccttcctgcg cgtgcggctt caggccgacg gcagggagat cgtccgcgag acgcccttcg
     9601 tcttcgtcgg caacaacgag tacgagatcg aaagctggca gatcggcgcg cgcacgcgcc
     9661 tcgacgcggg gacgctgagc ctctacgtca cgcaccgcaa ggggcgtctc ggcctcgcgt
     9721 ggctcgccgc gcgcgcgctc ctcggtcggc tgcgcgaggc gaaggacttc gacgcgctcc
     9781 gcgtctcgga agtcgtcgtc gagacgcacc gcgcgggcaa gcgcctcccc gtcgccgccg
     9841 acggcgaagt cgccctgatg aagacgccgc tccactaccg cgcgctgccg ggcgcgctgc
     9901 gggtcatcgt tccggaggaa gtgaagacat
//
LOCUS       DBNGOH010000209         2696 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2047527, whole genome shotgun sequence.
ACCESSION   DBNGOH010000209 DBNGOH010000000
VERSION     DBNGOH010000209.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2696)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2696)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2696
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2047527"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..572
                     /locus_tag="AC159P_04005"
     CDS             <1..572
                     /locus_tag="AC159P_04005"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HIR3694649.1"
                     /translation="VLLERTRDAEAVAEKLTYVEPRGDTALYDATYLAIERLARGAHR
                     RRVLLLLSDGEDNRSRYGFGDVRRSLEESDVIVYGVGIGTGYSRVATGRETLEKLSAT
                     SGGRAFFPDGGGDMDEAFERIALELRHQYSIGYKPSGLAGAGEWHSLNVKVTRPAGSP
                     RLFVRSRRGYYAFTGQKSSPAPVSDFVKQ"
     gene            688..1260
                     /locus_tag="AC159P_04010"
     CDS             688..1260
                     /locus_tag="AC159P_04010"
                     /inference="COORDINATES: protein motif:HMM:NF021587.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S-containing protein"
                     /protein_id="HIR3694650.1"
                     /translation="MSEEKRQQQEQGTSRESKRAQFTQPAGGGGRLKLAALVLALAGV
                     AAYLVFANVQGRDDSASPTKAAEVKPASDAKADEVRVPLKELEGGKAVFFEPKLASGK
                     PVRFFVVKATDNTYRAALDACEVCFHAKKGYEQKGSDMICRNCGRDFAIGDIGPHNEG
                     GCHPIALARTVEGDSLVIKTSELEKGSSYF"
     gene            1288..2562
                     /locus_tag="AC159P_04015"
     CDS             1288..2562
                     /locus_tag="AC159P_04015"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3694651.1"
                     /translation="MRISTIALANLRRRKGKAAFLVAGIAIGIATAVALLTLSGMIRE
                     EIGLQLDQYGANIVVVPKSNSLALDYGGVTVSGVAFDVNQLKDEDARRIHEIEYRNRL
                     AVVAPKILGAVEAEGRQVLLAGVDFEQELKLKRWWRLVGEKPQGESDLLVGYEVAQAL
                     SLVEHVMGDQSVPVVPTSPTTVDYAAAGRHAAHRDMSGHFRVVRDRLTLAGREFRVTG
                     VLTPTGAREDGVIFGQLQSVQALLGKPGQLSLVEVSALCQDCPVEDIVAQISRQLPNA
                     KVSAIQQAVRTRTETVERLTRFALAVSGVVLFIAALMIFTTMMGAVVERTKEIGVLRA
                     IGFRRAHIIKGLMLEVLLVSTLGGLVGWLAGVLASRALLPYFSQTGLEFAVEPKLALL
                     SVLAALAVGAASSIYPSLRASRLDPSEAVRYV"
BASE COUNT          493 a          847 c          929 g          427 t
ORIGIN      
        1 aagtcctgct ggagaggacg cgcgacgcgg aggcggtcgc cgagaagctt acctacgtcg
       61 agccgcgcgg ggatacggcc ctgtacgacg cgacgtatct ggccatcgaa aggctcgcgc
      121 gcggcgcaca ccggaggcga gtcctgcttc tcctcagcga cggggaagat aaccgctcac
      181 gttacggctt cggcgacgtt cggcgcagcc tcgaagagtc cgacgttatc gtctacggcg
      241 tggggatcgg cacgggttac tcccgcgtcg cgacgggccg ggagacgctc gaaaagctga
      301 gcgcgacttc cggcgggagg gccttcttcc ccgacggcgg cggcgacatg gacgaggcgt
      361 tcgagcgcat cgcgttggaa ctccgccacc agtattcaat cggctacaag ccgtcgggtc
      421 tcgcgggggc gggcgagtgg cactcgctga atgttaaggt gacgcggccc gcgggatcac
      481 cgcgcctgtt cgtgcggagc cgcagggggt actacgcctt cacggggcag aagtcttccc
      541 cggcacccgt ttccgacttc gtgaaacagt gaagtcgaaa cagtcgaaac acaagacaag
      601 gcatcaaaac gaggaattac gttcgggttc accaaccaga acggaaagcc ggggcgtcaa
      661 aacaggtcaa caatctttgg aggagtcatg agcgaagaga aacgtcaaca gcaggaacaa
      721 gggacctcgc gcgagagcaa gagggcgcag ttcacgcagc ctgcgggagg tgggggccga
      781 ctgaagctgg ccgccctcgt gctcgcgctc gcgggcgtcg ctgcctatct ggtcttcgcc
      841 aacgtgcaag gccgcgacga cagcgcgagc cccacgaagg ccgcggaggt taagcccgca
      901 tcggacgcga aggcggacga ggtgcgcgtc ccgctgaagg agttggaagg cgggaaggcc
      961 gtcttcttcg aaccgaaatt ggcgagcggc aagcccgtcc gcttcttcgt cgtcaaggcc
     1021 actgacaaca cctaccgcgc ggcgctcgac gcctgcgagg tctgcttcca cgccaagaag
     1081 ggctacgagc agaaaggctc ggacatgatc tgccgcaact gcgggcgcga cttcgccatc
     1141 ggcgacatcg gcccgcacaa cgagggcggc tgccacccca tcgcactcgc gcgcacggtc
     1201 gaaggcgatt cgctcgtgat caagacgagc gagctggaaa agggcagctc ttatttctaa
     1261 accgcccgcg cggcgaaggg tgaagtaatg cgcatcagca cgatagcact ggcgaatctc
     1321 aggcggcgta agggcaaggc ggcgttcctc gtcgcgggga ttgccatcgg catcgcgacg
     1381 gcggtggcgc tgctcacgct gagcgggatg atccgcgagg agatcggcct ccagctcgac
     1441 caatacggcg ccaacatcgt cgtcgtgccc aagtcgaata gcctcgcgct cgactacggc
     1501 ggcgttacag tgtcgggcgt cgccttcgac gtgaatcagc tcaaggacga ggacgcgcgg
     1561 cggattcacg aaatcgagta ccgcaaccgt ctggcggtcg tcgcgccgaa gattctcggc
     1621 gcggtcgagg cggaagggcg gcaggtgctt ctcgccggcg tcgatttcga gcaggagctg
     1681 aagctcaagc gctggtggcg tctggtcggc gaaaaaccac agggcgaaag tgacctgctc
     1741 gtcggctacg aagtcgcgca ggcgctctct ctcgtcgagc acgtgatggg agaccagtcc
     1801 gtccctgtcg tgccgacctc ccccacgacc gttgactatg cggcggcggg acgccacgcc
     1861 gcgcaccgcg acatgagcgg ccacttccgc gtggtgcgcg accgcctgac gctggccggg
     1921 cgcgagttcc gcgtgacggg cgtgctcacg ccgacgggcg cacgcgagga cggggtgatc
     1981 ttcggccagc tccagagcgt ccaggcgctc ctcggcaagc ccggccagtt gagcctcgtc
     2041 gaggtgagcg cgctctgtca ggactgcccc gtcgaggaca tcgtcgcgca gatcagtcgg
     2101 cagctcccga acgcgaaggt ctccgcgatc cagcaggcgg tgcgtacgcg caccgagacc
     2161 gtggagaggc tgacgcggtt cgcactagcc gtctccgggg tggttctctt catcgcggcg
     2221 ctgatgatct tcaccacgat gatgggggcg gtcgtcgagc gcaccaaaga gataggcgtg
     2281 ctcagggcca tcggcttccg gcgcgcccac atcatcaagg ggctgatgct ggaggtcctg
     2341 ctggtcagca ccctcggcgg cctggtgggc tggctcgccg gggtgctcgc gagccgggcg
     2401 ctcctgccgt acttcagcca gaccggcttg gaattcgcgg tcgagccgaa gctcgcgctc
     2461 ctgtccgtcc tggccgcgct ggcggtcggc gccgcgagca gcatttatcc gtccttacgt
     2521 gcctcgcgac tcgacccgtc cgaggccgtc cgctacgtgt gaaggggtga agaatgtcat
     2581 tcgtcgtcgt taaggacgtg agtaaggtct accaaacgga cggcgcctcc gcgccgacgc
     2641 gcgtgctcga cggcgtcaac gtcgaagtgc gcgacgggga gttcgtctca ttgatg
//
LOCUS       DBNGOH010000210         2548 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_214371, whole genome shotgun sequence.
ACCESSION   DBNGOH010000210 DBNGOH010000000
VERSION     DBNGOH010000210.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2548)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2548)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2548
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_214371"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..941
                     /gene="rlmN"
                     /locus_tag="AC159P_04020"
     CDS             <1..941
                     /gene="rlmN"
                     /locus_tag="AC159P_04020"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dual-specificity RNA methyltransferase RlmN"
                     /protein_id="HIR3694652.1"
                     /translation="EFRARLGERATASTLTVESRYISEDGTRRYLLKTHDGLPVETVF
                     IPEERRDTICFSSQSGCPLQCSFCLTAKLGLLRTLTAGEMVEQIVVALNDAYGVGAKT
                     PRGTNLVAMGAGEPFLAFDPLMKALRIMSDTEGLFIVPNRVTVSTAGVVPKIRELATV
                     EARPHLAVSLAAPTDELRDVLMPINKRWPLKELLAACREFQETLKPGELFTFEYVLLD
                     GVNDSDEHARRLANLLNRHGLKAKVNLIPHNPAEGLPYRPSPEERVQSFKHILEAKSV
                     RAFVRRPRGRDIYAACGQLAARRVSNAAAGVLHQLS"
     gene            982..1587
                     /locus_tag="AC159P_04025"
     CDS             982..1587
                     /locus_tag="AC159P_04025"
                     /inference="COORDINATES: protein motif:HMM:NF022853.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD N-terminal
                     domain-containing protein"
                     /protein_id="HIR3694653.1"
                     /translation="MRRQAFTLFAGAALIISAVLSPLGLKATAAPSMAAAAAAQNSSN
                     IAVNGFFSVDPANQGSSFRAAIVMDIPGGLHVNGNKPLGKYAIPTAVKIDAPKGLKVT
                     PVAYPAAKVRSFDFGSGTPERLAVYEGRAIMRFNVAVAPDAPIGVARVRANVRFQSCS
                     DTVCFPPATRELVLAIAIVGAGERVNNINAEYFGGGRGRKR"
     gene            complement(1739..>2548)
                     /gene="pgeF"
                     /locus_tag="AC159P_04030"
     CDS             complement(1739..>2548)
                     /gene="pgeF"
                     /locus_tag="AC159P_04030"
                     /inference="COORDINATES: protein motif:HMM:NF014619.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HIR3694654.1"
                     /translation="RAGFRWLEGVEGVRAIACGPLERAGFANAFSTRGGGVSPFPEGS
                     LNLAGFDQDAAENIHENRRRFFEVLGGGWTLAACWQVHGHDVHVVREVPDPRSEQEYC
                     DAVATRERGVLVGVKTADCVPVLLGDARTGACAAVHAGWRGTLAEIVKHALERMRAEF
                     GTDPCDVRAAIGPAALGCCYEVGAEVIDAFKEKFTRAPELFTPTREGHALVDLHRANR
                     EQLIEAGVAAERVHALPLCTMCRTDLFFSYRQDRKLYGRTGRLLSVIGRRQ"
BASE COUNT          421 a          912 c          826 g          389 t
ORIGIN      
        1 aggagttccg cgcgcggctc ggggagcgcg ccacggcctc gacgctcacc gtcgaatcgc
       61 gctacatctc cgaggacggc acgcgccgtt accttttgaa gacgcacgac ggcctgcccg
      121 tcgagaccgt tttcatcccc gaagagcggc gcgacacgat ctgtttttcg tcgcagtcgg
      181 gttgcccgct gcaatgcagt ttctgtctga cggccaagct cggcctgctg cggacgctga
      241 cggcgggcga gatggtcgag cagatcgtcg tcgccctgaa cgacgcgtac ggcgtcggcg
      301 cgaagacgcc gcgcgggacg aacctcgtgg cgatgggcgc gggcgagccg ttcctcgcct
      361 tcgacccgct gatgaaggcg ctccgcatca tgtccgacac ggaaggtctc ttcatcgtgc
      421 cgaaccgcgt gacggtctcg acggcaggcg tcgtgccgaa gatcagggag ctggcgaccg
      481 tcgaagcgcg cccgcacctc gccgtctcgc tcgccgcgcc gacggacgag ctgcgcgacg
      541 tgctcatgcc catcaacaag cgctggccgc tgaaagaact gctcgccgcg tgcagagagt
      601 ttcaggagac tttgaagccc ggcgagctgt tcacgttcga gtacgtcctg ctcgacggcg
      661 tgaacgactc ggacgagcac gcgcgcaggc tcgccaacct cttgaaccgc cacggcctca
      721 aggcgaaggt caacctgatc ccgcacaacc cggcggaagg gttgccctac cgcccttcgc
      781 ccgaagagcg cgtgcagtct ttcaaacaca tcctcgaagc gaagagcgtc cgcgccttcg
      841 tccgccgccc gcgcgggcgc gacatctacg ccgcctgcgg ccagctcgcc gcgcgccgcg
      901 tgagcaacgc cgccgcgggc gtgcttcatc aattgtcctg aagctcccct tccgacattc
      961 atcaagaaga aaacataccg catgagacga caagcattca cactattcgc cggcgccgct
     1021 ttaatcatct ccgccgtgct ctcgcccctg ggcctcaagg cgaccgccgc gccctccatg
     1081 gccgccgcag ccgcggcgca gaacagcagc aacatcgccg tcaacggctt cttctcggtc
     1141 gatccggcga accagggcag cagcttccgc gcggccatcg tgatggacat ccccggcggg
     1201 ctgcacgtca acggcaacaa gccgctcggc aagtacgcca tcccgaccgc ggtcaagatc
     1261 gacgcgccga aaggtctcaa ggtcacgccc gtcgcctacc cggcggcgaa ggtgcgcagc
     1321 ttcgacttcg gctccggcac gcccgaacgg ctcgccgtct acgagggccg cgccatcatg
     1381 cgcttcaacg tcgcggtcgc accggacgcg cccatcggcg tcgcccgcgt gcgcgccaac
     1441 gtccgcttcc agagctgctc cgataccgtc tgcttcccgc ccgccacgcg cgaactcgtc
     1501 ctcgccatcg ccatcgtcgg cgcgggcgag cgcgtcaaca acatcaacgc ggaatacttc
     1561 ggcggcgggc gcggcaggaa acggtaagcg actacgaaag cggtctcgtt tgcgagccgc
     1621 cacgcggcgc acgtcatgac tgtaggggca gggcttgtcc ctgcccgccg tcacgcgtcc
     1681 attcaacgtt gaatccgtcg aatcgcaacg ggcagggaca agccctgccc ctacaatttt
     1741 attggcggcg accgatgacg gagagcaggc gccccgtccg cccgtagagc ttgcggtctt
     1801 ggcggtagga gaagaagagg tctgtgcggc acatcgtgca gagcggcagc gcgtgaacgc
     1861 gctcagccgc gacgcccgct tcgattaact gctcgcggtt agcacggtgc agatcgacga
     1921 gcgcgtggcc ttcgcgcgtg ggcgtgaaga gttcgggcgc gcgggtgaat ttctctttga
     1981 aggcgtcaat gacctccgcg ccgacctcgt agcagcagcc gagcgcggcg gggccgatgg
     2041 cggcgcgcac gtcgcacggg tcggtgccga actcggcgcg catccgttcg agcgcgtgtt
     2101 tgacaatctc cgcgagcgtc ccgcgccagc ccgcgtggac ggcggcgcac gcgcccgtcc
     2161 gcgcgtcgcc cagcagcacg ggcacgcagt cggcggtctt gacgccgacg agcacgccgc
     2221 gctcgcgcgt ggcgacggcg tcgcagtatt cttgctccga gcgcgggtcg ggcacttcgc
     2281 ggacgacgtg cacgtcgtgg ccgtgcacct gccagcaggc cgcgagcgtc cagccgccgc
     2341 ccagcacttc gaagaagcgg cggcggttct cgtggatgtt ctccgccgcg tcctggtcga
     2401 agcctgcgag gttgagcgag ccttcgggga aggggctcac gccgccgccg cgcgtcgaga
     2461 aggcgttggc gaaccctgcg cgctccaacg gcccgcacgc aatcgcgcgc accccttcga
     2521 ccccttcgag ccagcggaag ccggcgcg
//
LOCUS       DBNGOH010000211         3045 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_764590, whole genome shotgun sequence.
ACCESSION   DBNGOH010000211 DBNGOH010000000
VERSION     DBNGOH010000211.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3045)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3045)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3045
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_764590"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..313)
                     /locus_tag="AC159P_04035"
     CDS             complement(<1..313)
                     /locus_tag="AC159P_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012920131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HIR3694655.1"
                     /translation="MRETTAQETTAKAGGARGAGRKAQRARTRQANLEAAVDLASVEG
                     LEGLTIGRLAAALSMSKSGLFAHFGSKEELQLATVEAARAVFIREVIRPAFEAARGLP
                     RL"
     gene            698..2140
                     /locus_tag="AC159P_04040"
                     /pseudo
     CDS             698..2140
                     /locus_tag="AC159P_04040"
                     /inference="COORDINATES: protein motif:HMM:NF019556.7"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
     gene            2150..2968
                     /locus_tag="AC159P_04045"
     CDS             2150..2968
                     /locus_tag="AC159P_04045"
                     /inference="COORDINATES: protein motif:HMM:NF042854.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6805 domain-containing protein"
                     /protein_id="HIR3694656.1"
                     /translation="MSVRTEAVPDDANKVAFLYGPVVLAGDLGPVAAGPTVPYAADHL
                     VNLRAPVAEVPVLVTGAENRAGLLRRVRAGDLVFRAVASGLEGPPRGVTLRPFNEIFY
                     EHYNVYWDVLTPSGYAERRASLRAEAARQAALDARTLDEYRPGEQQSEVEHAQKGEKT
                     SAGEWQYRKLRHATDGGWFSFRMKVAPDAPSELVFTYWGGETGNRTFDILVDGKMIAT
                     ESLSRDKPGAFFDKTYPIPAELTRGKRAVEVRLQARPGNYAGGLYGARTLRARP"
BASE COUNT          483 a         1050 c         1055 g          457 t
ORIGIN      
        1 acaggcgcgg cagcccgcgc gcggcctcga aggcggggcg gatgacctcg cggatgaaga
       61 cggcgcgcgc cgcctccacg gtggcgagct gcaactcctc tttggagccg aagtgcgcga
      121 acagtccgct cttgctcatc gagagcgccg cggcaagccg cccgatggtc agaccttcga
      181 ggccctcgac cgaggccaga tcgaccgcgg cctcaaggtt cgcctgccgg gtccgcgcgc
      241 gctgcgcctt gcgccccgcg ccccgcgccc cgcccgcctt cgccgtcgtt tcttgtgccg
      301 tcgtctcgcg caccaaacac ctcgcctgcc gtgatgggat tcatatagca cgaacgttcg
      361 ttttagagca agtcccagcc gtccgccgcc cgcccgccgc cgcccccgcc gccggtgcgc
      421 gttccgattt tagttatgtc cgcgagccgc gttacaatcg cccgtacatg aattcgaaga
      481 aacgtggtga ggcatgaagg tgtttcccgc aaatctccgg ctggctcgcg cgctgctcgg
      541 cgccctcctg ccgctcgcag ccgtcgcggc gacgcttcga actccgtccg ttgcccttcg
      601 acgaaacccc gacccgtcgg tctcttccac gcaaaagccc ctcggcgtaa agcagttctc
      661 tccgaccgcg gagcctttcg cgctacggca ggtggagttg ctcgacagcc ccttccgcgc
      721 ggcgatggag cgtaacgcga agtacctgct cgcgctcgac cccgaccgcc tgctgcacaa
      781 cgcgcgcagg tacgcgggcc tcggaccgaa aggcgagctg tacggcgggt gggaggcgcg
      841 cggcatcgcg ggccactcgc tgggccatta cctgacggcg ctctcgctgc aatacgcggc
      901 cacgggcgac gcgcgcttcc gcgagcgcgt ggcttacatc gtcggggagt tggccgagtg
      961 ccagcgcgcg tacggcgacg ggtacgtcgg cgcgctgccg ccgctggagc ttcggacgct
     1021 gcgcggcttc aaggacggta aggtcgaagt gcagggcagc ttcaacttca agggcggcgc
     1081 gtgggtgccg tggtacacac agcacaaggt gctcgccggg ctgaaggacg cgtggacgct
     1141 ggcgggcgac gagcaggcga agcaggtcgc gctcgcgctg gccgactggg tcgaggtcgt
     1201 gacgcgcccg ctcacgccgg agcagcggca gcagatgctg cgcgtcgagt tcggcgggat
     1261 gagcgagacg ctggccgacc tctccgcgct gacgggcgaa gagaaatatc tcgacgcctc
     1321 gcgccgcttc tatcacgaag ccatcctgcg cccgctcgcg gaaggacgcg acgagctggc
     1381 gggcaaacac gccaacacgc aaatcccgta ggtcatcggc gaggcgcggc gctacgaggt
     1441 cgcgggcgac gaagagggtc gcagggtcgc ggaagttttc tgggagcgcg tggtcgatca
     1501 ccacacgtac gtcatcggcg gcaacagcga gcacgagcac ttcggccagc ccgaccgtct
     1561 ggcggagcgt ctgggtcagg ccaccgccga gtcgtgcaac acctccaaca tgctcaagct
     1621 cacgcgccac ctcttcgcgt ggcggcccga ggcgcggcac ttcgactact acgagcgcgc
     1681 gctctacaac cacatcctcg cgtcgcagga gccgcggcgc ggcatgttcg cctacttcat
     1741 gtcgctccgc cccggccact tcaagaccta cagctcgccc gacgattcct tctggtgctg
     1801 cgtcggctcg gggatggaga accacacgaa gtacaacgac tcgatctatt ttcacggcgc
     1861 cgactcgctg tacgtgaacc tcttcatccc ttcgcggctc aagtgggaag agaaggggct
     1921 ggtgctcgaa cagcagacgg cgtacccgcg gggcgggcgc gtcgagctgc gcttgaagac
     1981 gaaggagccg gtgccgctcg tcctcaaagt gcggacgccg cagtgggccg cgggcgggtt
     2041 gcggtttcgt ctgaacggga ggcccttgcg ggtcgagtcg cgccccggca gctacgcgga
     2101 agtcagccgc acgtggaaga cgggagacgt gctgcaagtg acgattccga tgtccgtgcg
     2161 cacggaggcc gtgccggacg acgcgaacaa ggtcgccttt ctttacggcc ccgtcgtgct
     2221 ggcgggcgac ctcgggccgg tggccgcggg gccgaccgtc ccttacgcgg cggatcatct
     2281 ggtcaacctg cgcgcgcccg tggcggaagt ccccgtgctg gtgacggggg ccgaaaaccg
     2341 cgcgggcctc ttgcggcgcg tgcgtgccgg cgacctcgtg ttccgcgccg tcgcgtccgg
     2401 tctggagggg ccgccgcgcg gcgtgacgct gcgccccttc aacgagattt tctacgagca
     2461 ctacaacgtc tattgggacg tgctgacgcc ctcgggttac gcggagcgca gggcgtcgct
     2521 gcgtgcggag gccgcgcggc aggcggcgct cgacgcgcgc acgctcgacg agtaccgacc
     2581 gggcgagcag cagtcggaag tcgagcacgc gcagaagggc gagaagacct cggcgggcga
     2641 atggcagtac cgcaagctgc gccacgcgac ggacggcggg tggttctctt tccggatgaa
     2701 ggtcgcgccc gacgcgccgt ccgaactcgt cttcacctac tggggcggcg agaccggcaa
     2761 ccgcaccttc gacattctgg tggacggcaa aatgatcgcg accgagagcc tgagccgcga
     2821 caagccgggc gcgttcttcg acaagaccta cccgattccg gcggaactga cgcgcggcaa
     2881 gcgggccgtc gaggtgcgcc tacaggcccg ccccggcaac tacgcgggcg gcctctacgg
     2941 ggccagaacc ctgagagcgc gcccctgatt cggcgcgcgg gcctgccggg cgccgcccgc
     3001 tgccctcggc ggcgcggcgg gggcgtgccg aatgtgcaac gaaaa
//
LOCUS       DBNGOH010000212         2780 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2781511, whole genome shotgun sequence.
ACCESSION   DBNGOH010000212 DBNGOH010000000
VERSION     DBNGOH010000212.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2780)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2780)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2780
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2781511"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..659
                     /locus_tag="AC159P_04050"
     CDS             <1..659
                     /locus_tag="AC159P_04050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694657.1"
                     /translation="EQEDDAATASDHTTPPGGGDKKDPPPLDGEKAADTGDHRRVSSA
                     EYLITEIKKHRKAATLLLALLALALAFAVYAMLPLRTPVDSVAILPFVNGVGPELETF
                     GDGLTDSVINNLTTAGVKVKSRHAAFSYKGKGMDPQTIGRELGVRAVLTGSIQKQGDA
                     LVIKAALVNVADDFQLWGKQYPVYDLVGGGRFLALQEDISKQITDEMKTKIINADKKP
                     "
     gene            complement(794..1555)
                     /locus_tag="AC159P_04055"
     CDS             complement(794..1555)
                     /locus_tag="AC159P_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011197293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HIR3694658.1"
                     /translation="MPDIDIKPVRVAEGAGRVRVRVLLFGAAREAAGADELTLDFAAP
                     ATARDVFERVTADYPKLRRFGRSLLVAVNQEYARELGGEVKDGDEVAVFPPVSGGAGD
                     AGAGEADDARDFFELTTEPIDVGSVARRVVPRRCGATVTLDGYAREWTRGRRTLHLEY
                     EAYAPMALSEMKRLGEEAHRRFPIAHLGIVHRTGRVEIGETSVVIAVSAPHRRAAFEA
                     CEWAIKELKRTVPVWKKEFFEDGEVWVEGEGASQA"
     gene            complement(1652..2356)
                     /locus_tag="AC159P_04060"
     CDS             complement(1652..2356)
                     /locus_tag="AC159P_04060"
                     /inference="COORDINATES: protein motif:HMM:NF014269.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HIR3694659.1"
                     /translation="MSEALDKVRGVRELPVFPLPLVLFPGVPLPLHIFEPRYRRMLAD
                     VRAGNSLFGLSYFDAEESAGDRPAPGHVGCAAEVVEVQPQPDGRSNILTVGLARYRVE
                     TYVERGDPYLVARVEFFEDEDEDDRLLRRRAREVSEVFTRIARAVRTLNDDRAAPPDL
                     SDAEPERLSFLVAAAMEMDTAVKQELLELRSGAERLQRLHGLLSQAVETYERRARVHT
                     VARGNGHAGAKIDIEE"
     gene            complement(2486..>2780)
                     /locus_tag="AC159P_04065"
     CDS             complement(2486..>2780)
                     /locus_tag="AC159P_04065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694660.1"
                     /translation="AGLDADTSRQERERRNVEDLIARDNRIRDHAREMAELVLEAKRR
                     ALGDDTPADILELIEQKLAPRPRDERAGGDAAAAAPPRPRCKRSNAARNKPSQ"
BASE COUNT          477 a          914 c          935 g          454 t
ORIGIN      
        1 ccgaacagga agatgacgcg gcgacggcgt ccgaccacac aacgccgccg ggcggcggcg
       61 acaaaaaaga cccacccccg ctcgacggcg agaaggcggc ggacacgggc gaccatcggc
      121 gggtttccag cgcggaatac ctcatcaccg agatcaaaaa gcaccgcaag gccgcgacct
      181 tgctcctggc gctgctggcg ctggcgctcg cgttcgccgt ctatgccatg ctgcctctca
      241 ggacgccggt ggactcggtc gcgatcctgc cgttcgtgaa cggcgtcggc ccggaactgg
      301 agactttcgg cgacggtctg accgacagcg tgattaacaa cctgacgacc gccggcgtca
      361 aagtgaaatc acgccacgcc gccttcagct ataaaggcaa ggggatggat ccgcaaacaa
      421 tcgggcgcga gctgggggtg cgtgccgtcc tcaccgggag catccagaaa cagggcgacg
      481 ctctggtcat caaggctgcg ttggtgaatg tcgccgacga ttttcagctc tggggcaaac
      541 agtatccggt ctacgatctc gtcggcgggg gtaggttctt agccttacag gaagacatct
      601 ccaagcagat taccgatgaa atgaaaacga aaataatcaa cgcggataag aagccataag
      661 ctcgcgccag gcacagcggc aaagcgcacc accggcgtgg ataaataccg acgccgcgcg
      721 gcgtgccccg accggagtcg aacttcggat tagaatcctt gaagagcaag acttgctttt
      781 ccccacccac gcctcacgcc tgacttgccc cctcgccttc gacccagact tcgccgtctt
      841 cgaagaactc tttcttccag acggggacgg tgcgtttgag ttctttgatg gcccactcgc
      901 aggcttcgaa ggcggcgcgg cggtggggcg cgctgacggc gatgacgacc gaggtctcgc
      961 cgatctcgac gcggcccgtg cggtggacga tgccgaggtg cgcgatgggg aagcggcggt
     1021 gcgcctcttc gccgaggcgc ttcatctccg agagggccat cggcgcgtag gcttcgtatt
     1081 cgaggtggag cgtgcggcgc ccgcgcgtcc actcgcgcgc gtagccgtcg agcgtgacgg
     1141 tcgcgccgca gcggcgcggg acgacgcggc gcgcaacgct ccctacgtca atcggctcgg
     1201 tggtcagctc gaagaagtcg cgcgcgtcgt cggcctcgcc cgcgcccgcg tcgcccgcgc
     1261 cgccgctgac gggcgggaag accgcgacct cgtcgccgtc cttcacttcc cctccaagct
     1321 cgcgcgcgta ctcctgattg accgcgacga gcagcgagcg accgaagcgc cgaagcttgg
     1381 gatagtccgc cgtcacccgc tcgaaaacgt cgcgcgccgt cgcgggcgcc gcgaagtcga
     1441 gcgtcaactc gtccgccccc gccgcctcgc gcgccgcccc gaacaacagc acgcgcacgc
     1501 gcacgcgccc cgcgccctcc gcaactctca caggcttgat gtcgatgtca ggcataaaga
     1561 ttgggtattg ggtgttaggt gttgggtatt gggtaaaaac aaaagcaggt tcttacccaa
     1621 cacctaacac ccaataccca acacctgctt ttcattcctc gatgtctatt ttcgcgcccg
     1681 cgtggccgtt gccgcgggcg acggtgtgga cgcgcgcgcg gcgctcgtag gtttcgacgg
     1741 cctgcgagag gagtccgtgg aggcgttgca ggcgctcggc gccggagcgc agctcaagca
     1801 gctcctgctt gaccgcggtg tccatctcca tcgccgcggc gacgaggaac gagaggcgct
     1861 ccggctcggc gtcggacagg tcgggcgggg cggcgcggtc gtcgttgagc gtgcgcaccg
     1921 cgcgcgcgat gcgcgtgaag acctcggaga cctcgcgcgc gcgccggcgg agcagccggt
     1981 cgtcctcgtc ctcgtcctca aagaactcga cgcgcgcgac gaggtacggg tcgccgcgct
     2041 cgacgtaagt ttcgacgcgg tagcgcgcga ggccgacggt caggatgttc gagcgcccgt
     2101 cgggctgcgg ctgcacctcg acgacctccg cggcgcagcc gacgtggccc ggcgcgggcc
     2161 ggtcgcccgc agactcttcc gcgtcgaagt aggagaggcc gaagaggctg ttcccggcgc
     2221 gcacgtccgc cagcatccgg cggtagcgcg gctcgaagat gtgcagcggc agcggcacgc
     2281 ccggaaacag caccagcggc agcgggaaca cgggcagctc gcgcaccccg cgcactttgt
     2341 cgagagcctc ggacataaaa gactgtgaat ggtgaatagt aaatggtgaa tggaagaatc
     2401 tgtcgctgtt cgcgcttgcg atttagcgct cgctattatt cgacccgcga cgacgattca
     2461 ccatttacca ttcactattc accatttact gagacggctt gttccgcgct gcgttcgagc
     2521 gcttgcagcg cggtcgcggg ggcgcggcag cggcggcgtc cccaccggcg cgctcgtcgc
     2581 gggggcgcgg cgcgagtttc tgctcgatca gttcgaggat gtcggcgggg gtgtcgtcgc
     2641 cgagggcgcg gcgcttggct tcgaggacga gttcggccat ctcgcgcgcg tggtcgcgga
     2701 tgcggttgtc gcgcgcgatg aggtcttcga cgttgcggcg ctcgcgctcc tgccgcgagg
     2761 tgtcagcgtc gaggccggcg
//
LOCUS       DBNGOH010000213         3684 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3697821, whole genome shotgun sequence.
ACCESSION   DBNGOH010000213 DBNGOH010000000
VERSION     DBNGOH010000213.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3684)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3684)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3684
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3697821"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(162..3155)
                     /locus_tag="AC159P_04070"
     CDS             complement(162..3155)
                     /locus_tag="AC159P_04070"
                     /inference="COORDINATES: protein motif:HMM:NF039983.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6079 family protein"
                     /protein_id="HIR3694661.1"
                     /translation="MKRIQEKIKDLIEPHAYDAVRDFAAEPARALAAYRFTDMTSDLL
                     ARWVDALSSVGRGRGAAHALAGARGVGKSHTLAVFGAIACSESLARAVDDAHVATTAQ
                     RLARRRFAVVRVERGTRATLQEEMAAGFARALGGGESQWTGDPREMLAVAASRDPEAT
                     LVVLVDTASTRPSRVSRDDGPLLGEMAAATRGTAAFVALALDDDIAGADGANVALSGT
                     YRIDYLGAEHLYHVADQHVLCKKPQARSAIHEIYSSLRATVSGFNWSESRFALLYPVH
                     PLIADVASAVRLYVPHFSFLPFAASVARHAAGRPALSLVLLDELFDGVESDLRKSTEL
                     RGAFAAYDHLLAESVERLPVMQRYQARLLLKSLFILSLDGRGATARELCAALLLPDDD
                     AQVAGQVAETLERFTGAALPASLQVGAREGDAAAANYRFRLDATEAQVPALEGDDEAH
                     EAAVEQDAPEREDDDAAQAGRASTGSSSAADLLNDLESEGLFDPPGVSASTGANASAE
                     AEATASEAKDAEAPNAEAANAEPTATNTTDVRATDEEAASAGTAQAGTTEASADATEA
                     CADVAAPVAREASKVEPVIESVRAVTEDERRDPEELTEWARYLTEQPSLGSITDEGGR
                     EVIREALSAWLRKWRGLELEKRIEALPDAALNTRVWDLGRLVRRRFDAAAEAVEAALA
                     EKTSLEEGLVRASEAFDRSPGKLASAARHLADLRDYVNDFAPTERVRAYLATAEPTGV
                     ERIEAARRELLSIAADAGALLERERRERLRVLWREFHTHYVEHYAALHDKTMACGDRE
                     EFQSLTGGDRWREFEALSRLTVVSAQPRRRVDELLERERAARCALPVRRLLETQPSCA
                     CAFRLSRAAEFERLARDLEESVEGGIEVYRRTLLLMAGHLAIALDALARKEEDAESAR
                     RARSLSTAFAQKRLPERFALADVRFVERALKRTQLPPVRVAAPNGENGALTRDQLRTR
                     LDGWLDELPEQPVLIELIGKETHAP"
BASE COUNT          495 a         1293 c         1301 g          595 t
ORIGIN      
        1 aaaaggggaa aaggggaaga ggggaaaagg gagaaaactg ttcttccctc ttcgcctttt
       61 cccctcttcc ccttttcccc tttcctgttc ccctcttccc cttttcccct cgcgccttcg
      121 cacgtccgcc ttctgatttc agccttcgga cttctgactt cttacggggc atgtgtctct
      181 ttaccgatga gttcgatgag cacgggttgt tcgggcaact cgtcgagcca gccgtcgagg
      241 cgcgtgcgca actggtcgcg cgtgagcgcg ccgttctcgc cgttgggcgc ggcgacgcgg
      301 acgggcggga gctgcgtccg cttcagcgcg cgctcgacga agcgcacgtc ggcgagcgcg
      361 aagcgttcgg gcaggcgctt ctgcgcgaac gcggtcgaga gcgagcgcgc gcgccgcgcc
      421 gactcggcgt cctcctcttt gcgcgcgagc gcgtcgagcg cgatggcgag gtggccggcc
      481 atcagcagca gcgtgcggcg gtagacttcg atgccgccct cgacgctctc ttcgaggtcg
      541 cgcgcgaggc gttcgaactc cgcggcgcgc gagaggcgga acgcgcacgc gcacgacggc
      601 tgcgtttcga gcaggcgccg cacgggcagc gcgcaacgcg ccgcgcgctc gcgttcgagc
      661 agctcgtcga cgcggcggcg cggctgcgcg ctcacgaccg tgaggcgcga gagcgcctcg
      721 aactcgcgcc agcggtcgcc gcccgtgagc gactggaact cttcgcggtc gccgcacgcc
      781 atcgttttat catgaagcgc ggcgtaatgc tcgacgtagt gcgtgtggaa ctcgcgccag
      841 aggacgcgca gccgctcgcg ccgctcgcgt tcgagcaagg cccccgcgtc cgccgcgatg
      901 gagagcagtt cgcgccgcgc cgcctcgatg cgctcgacgc cggtcggctc ggcggtggcg
      961 aggtaggcgc gcacgcgctc ggtgggcgcg aagtcgttga cgtagtcgcg caggtctgcg
     1021 aggtgccgcg cggcggaggc gagcttgccc ggcgagcggt cgaacgcttc gctcgcgcgc
     1081 acgagtcctt cttcgagcga ggtcttttcg gcgagcgcgg cctcgaccgc ttccgccgcc
     1141 gcgtcgaagc gacggcgcac gagccgtccc aagtcccaca cgcgcgtgtt gagcgcggcg
     1201 tcgggcaacg cctcgatcct cttttcgagt tccagcccgc gccacttgcg gagccacgcc
     1261 gagagggctt cgcggatgac ctcgcgcccg ccctcgtcgg tgatcgagcc gagcgaaggc
     1321 tgctccgtca ggtaacgcgc ccactcggtc agctcctcgg ggtcgcgccg ctcgtcttcc
     1381 gtaaccgcgc gcacgctctc gatgacgggt tcgaccttcg atgcttcccg cgcgaccggg
     1441 gccgcaacgt ccgcgcaggc ctccgtcgca tcggcgctcg cctccgtagt ccccgcctga
     1501 gctgtgcccg cgctcgccgc ctcttcgtcg gtcgcgcgca cgtccgttgt gttcgtcgcc
     1561 gtcggctcgg cgttcgcggc ttcggcgttc ggcgcttccg cgtccttcgc ttcggaggcg
     1621 gtcgcctccg cctccgcgct cgcgttcgct cccgtgctcg cgctcacgcc cggcgggtcg
     1681 aacagccctt cgctttcgag gtcgttgagg aggtcggcgg cggacgacga gcctgtgctc
     1741 gcccgacccg cctgagccgc gtcgtcgtct tcgcgctccg gggcgtcctg ttcgaccgcg
     1801 gcttcgtgcg cttcgtcgtc gccttcgagg gccgggactt gagcttccgt cgcgtcgagt
     1861 cggaagcggt agttggccgc ggcggcgtcg ccctcgcgcg cgccgacttg caggctggcg
     1921 ggcagcgccg cgcccgtgaa acgttcgagc gtctcggcga cctgcccggc gacctgcgcg
     1981 tcgtcgtccg gcaggagcag cgccgcgcac agctcgcgcg ccgtcgcgcc gcgcccgtcg
     2041 agcgagagga tgaagagact cttcagcagc agccgcgcct ggtagcgctg catgacaggc
     2101 aggcgctcga cgctctccgc gagcaggtgg tcgtaggcgg cgaaggcgcc gcgcagctcg
     2161 gtcgatttac gcaggtctga ctcgacgccg tcgaacagct cgtccaggag gacgagcgag
     2221 agcgcggggc gcccggcggc gtggcgcgcg accgaggcgg cgaacgggag gaaggagaag
     2281 tgcgggacgt agaggcgcac cgccgaggcc acgtcggcga tgagcgggtg gacggggtag
     2341 agcagcgcga agcgcgactc gctccagttg aagccggaga cggtcgcgcg caaggaggag
     2401 tagatttcgt ggatcgcgga gcgcgcctgc ggcttcttgc agagcacgtg ctggtcggcg
     2461 acgtggtaga ggtgctcggc gccgaggtag tcgatgcggt aggtgcccga gagcgcgacg
     2521 ttcgcgccgt cggcgcccgc gatgtcgtcg tcgagcgcga gcgcgacgaa ggcagccgtg
     2581 ccccgcgtcg ccgcggccat ctcgccgagc agcggcccgt cgtcgcggct cacgcgcgaa
     2641 gggcgcgtcg aggccgtgtc caccagcacg accagcgtcg cctcggggtc gcgcgaggcg
     2701 gcgaccgcga gcatctcgcg cgggtcgccc gtccactgcg actcgccccc gccgagagcg
     2761 cgcgcgaagc ccgcggccat ctcttcctgg agcgtcgcgc gcgtcccgcg ctcgacccgc
     2821 acgaccgcga agcggcggcg cgcaagcctc tgcgcggtcg tcgccacgtg cgcgtcgtcc
     2881 accgcccgcg ccagactctc cgagcaggcg atggcgccga agaccgccag cgtgtggctc
     2941 ttgccgacgc cgcgcgcgcc tgcgagcgcg tgcgccgcgc cgcgcccacg gcccacgctc
     3001 gacagcgcgt ccacccagcg cgcgagcaag tccgaggtca tgtccgtgaa ccggtaggcg
     3061 gcgagcgccc gcgccggctc ggcggcgaaa tcccgcaccg cgtcgtatgc gtgaggctcg
     3121 attaagtcct tgatcttttc ctgtatgcgc ttcattcccg ggatcggacg gcgaaagggc
     3181 gggcaggcct tctattggcg ccgctcgcgg caaaactcgt ttgttgaaga gggtccttta
     3241 tagcttccgc aaaggtgagg cggaaggagg ggccatttcg aatctagcac gcggcggcgg
     3301 cggcgcgcaa tcactatctt ttttccggac ggctgctata atgcttccag attattcgtc
     3361 ggaaaacgga ctcgaacggg ggccgagcgc cgcgccgaaa aagtgggcgc gatgcggcgc
     3421 aacctcttga gagaaaaggc gcatcaaaga gacacgccac gcccggcgct cgggcactca
     3481 aggtttcggg tctctctggc agcagcggcg ggcgcgctcc ttatccgaat cggcagtcgc
     3541 cgcccgcgac cgcaccgccc gtctctcgcc gagagacggg gcggtttcgg tgcgcggccc
     3601 gcagtcgcac aaacgagttt tcggaggctt ttcccaaatg cgtaagaatt tttcccgcac
     3661 gctcaccgcc tcggctgtcg tcct
//
LOCUS       DBNGOH010000214         6410 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4980718, whole genome shotgun sequence.
ACCESSION   DBNGOH010000214 DBNGOH010000000
VERSION     DBNGOH010000214.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6410)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6410)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6410
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4980718"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(64..2118)
                     /locus_tag="AC159P_04075"
     CDS             complement(64..2118)
                     /locus_tag="AC159P_04075"
                     /inference="COORDINATES: protein motif:HMM:NF018757.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA domain-containing protein"
                     /protein_id="HIR3694662.1"
                     /translation="MSLLNPLFLLGLAAIAAPILVHLVRRTRARRVEFPALYFVRQVP
                     QRTIRRRTLHNLLLLILRCLAVLLLVFAFTRPFFSGAGAAKETEETRASVVLVDSSLS
                     MRQGEHFAEAKRRAEAIIGEAQADERVAVVAFGERYEAVGPFTADKKVAHAALAGLEV
                     GYEATDYEQALRGAEALLGALKTAGLKRVFLVSDFQATGWDAAKSTFKLSYDVRLVPI
                     DVGGADAAANVAVVNVEARGTVYGAKYADKLAVHVTNFSDEARERLTIDFQLNEQTVE
                     KREVALAPREAKVVEFTDFNLAEGPNRCVVSVRAEDFAYDNQFYFTLRGEKPGKALII
                     DSAVRGRGESFYLQSALATGENLPFAFTAKNAGAVDPAQLQEHALVVLNDAGALPVAL
                     GAAVTNFVEAGGQLIIATGPHTRAESFNGAFQRIAPAALGEMVQLQRGESVAITDVKM
                     SHPVFEVFRGSGRLASARVFGYHRTEPRERAQVLARFEDGSPALVEGAFGAKGRVLLF
                     ASTLGMTWNDLPLTPTYLPLVQQMVRYLGERETPAWHRLGQTFAVGKEREGAQPPAID
                     APSGARLKAGQLTPEGELLVTGREPGFYRLRYEARPDFAAVNVDGAEGDFTKLDLEAF
                     VAAVAGGDAVPAAADAEGRMSGAEIEARQRVWWPLLLAALLLFAAEALLARRTKMVKM
                     IG"
     gene            complement(2224..3201)
                     /locus_tag="AC159P_04080"
     CDS             complement(2224..3201)
                     /locus_tag="AC159P_04080"
                     /inference="COORDINATES: protein motif:HMM:NF013998.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HIR3694663.1"
                     /translation="MWWRRNRKSAAEAVADESAAAAPSAAPGPARFLDPEVLARIGSL
                     ELLARAVVEGFILGLHRSPFTGFSTEFTEYRQYNPGDDLRYLDWRLLGRTDRYFIKKY
                     RADTNTQCHLLVDTSASMRYASDGRVTKLQYAQFLAAALAHLVARQQDAVGLVGFGDK
                     VHTHVPARNRTGHMRTIYGQLAALEAGGETRLAEALHETAERLARRGLVCLVSDFYDQ
                     PERLSDAFRHLRFKGHDIIAFHVLDRNEVEFDFDDAVLLLEDAETAEQLPVLPDVVAK
                     GYRERIAAHVEEMRRCCAANHVDYEMITTDQPLDFALFSFLSRRAGKQK"
     gene            complement(3269..3832)
                     /locus_tag="AC159P_04085"
     CDS             complement(3269..3832)
                     /locus_tag="AC159P_04085"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015157349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3694664.1"
                     /translation="MTGRVDYVIRKATLDERDAIEELIAESARSLSRADYTDEQIEAA
                     ITSVFGVDTDLILDGTYFVAEAEGRLVGCGGWSRRRTLFGGDRYAARDSGELDPAAEP
                     AKIRAFFVHPAWARRGVGRAILETCEREASEHGFRALELMATLPGVRLYAARGYEGDE
                     RVEYDAGGGVKIEFVPMRKDLKQRTED"
     gene            complement(3829..4848)
                     /locus_tag="AC159P_04090"
     CDS             complement(3829..4848)
                     /locus_tag="AC159P_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR3694665.1"
                     /translation="MAVETTVMRDEELLDRLASAREKLLREIRKAIKGQDDVVEQVLL
                     SLFAGGHSILTGVPGLAKTLLVRTVSSVLDLSFKRIQFTPDLMPADITGTEIIEEDRA
                     TGRRAMEFIRGPVFANIVLADEINRTPPKTQAALLEAMQEGRVTVQGVSYELPRPFHV
                     LATQNPIEMEGTYPLPEAQLDRFMFQIRVGYLPEEDEVEVVKQTTAPQDYEFEPQMSA
                     EEIIAFQRLVRQVPASDTVARYAVRLVGASRPGTPAAPDFVNRWVTWGASLRASQFLI
                     LGGKARAVLRGRYNVSCEDVRAVAPAVLRHRVLLNFQAEAEKIDSDAVVKKLIEAVPE
                     PKSGL"
     gene            complement(4946..>6410)
                     /locus_tag="AC159P_04095"
     CDS             complement(4946..>6410)
                     /locus_tag="AC159P_04095"
                     /inference="COORDINATES: protein motif:HMM:NF024876.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase MA family metallohydrolase"
                     /protein_id="HIR3694666.1"
                     /translation="RMEEGRAAVEKSFAGDPFNVWAKNTLDLLDTMSEYRETKRGQFV
                     VKAAATESDVLGPYAADLLEEAAAKLTAKYRFTPQGPVYVELFPNHEDFAVRALGLPG
                     LGALGVCFGPVIAQDSPSARPAGEFNWGSTLWHEYAHVITLQMTDYRIPRWFSEGISV
                     YEERRARRGWGDDWNASVLRAHADGRWFKIADLDSGFMRPKRPDDISLAYFQASQVVE
                     FVVERHGFDAILKMLALYRDKARTPEVLRQALKLSEAEFDRAFAEYIEAKARPLQLAM
                     GTAGAEVAQLPKDEVLKRLGTQETFALHLRAGALYQAEGDAASAARHYKRGAELFPYY
                     TGPGNAYESLADIFEKQGDTGEAALALELLVKLDENNLDALKRLAELRLKQNDAARAL
                     DALRLSFYVSPFEHAPHARAGEILLARADAAAALREFQAALALQPPNEAEANYNVARA
                     FHALGKQADAKRSVLRALEAAPSYEKAQELLLTITGQ"
BASE COUNT          990 a         2232 c         2119 g         1069 t
ORIGIN      
        1 ggtcagtgaa aaccttcttg cctttcactg accactgacc actgaccacc ggccactgtt
       61 gattcatccg atcatcttca ccatcttcgt ccgccgcgcg agaagggcct cggcggcgaa
      121 gagcagcagc gcggcgagga gcagcggcca ccagacgcgc tgtcgcgctt cgatctccgc
      181 gccgctcatc cggccttcgg cgtccgccgc cgccggcacc gcgtcgccgc ccgcgaccgc
      241 cgccacgaac gcttcgaggt cgagcttcgt gaaatcgccc tcggcgccgt ccacgttgac
      301 cgccgcgaag tcgggccgcg cctcgtagcg cagccggtaa aatcccggct cccgccccgt
      361 cacgagcagc tcgccttcgg gcgtcaactg ccccgccttc agccgcgcgc ccgacggcgc
      421 gtcgatcgcg ggcggttgtg cgccctcgcg ctccttcccg accgcgaacg tctggccgag
      481 ccggtgccaa gcgggcgtct cgcgctcgcc gaggtagcgc accatctgct gcacgagcgg
      541 caggtaggtc ggcgtcagcg gcaggtcgtt ccaggtcatg ccgagcgtcg aggcgaagag
      601 cagcacgcga cccttcgcgc caaacgcccc ctcgacgagc gcggggctgc cgtcctcgaa
      661 gcgcgcgagc acctgcgccc tctcgcgcgg ctccgtgcgg tggtagccga agacgcgcgc
      721 cgacgcgagc ctgccgctgc cgcggaagac ttcgaacacg gggtgcgaca tcttcacgtc
      781 ggtgatcgcc acgctctcgc cgcgttgaag ctgcaccatc tcgccgagcg cggcgggcgc
      841 gatgcgctgg aacgcgccgt tgaaactctc tgcgcgcgtg tgcggccccg tcgcgatgat
      901 gagctggccg ccggcctcga cgaagttcgt gacggctgcg cccagcgcga ccggcagagc
      961 gcccgcgtcg ttgaggacga cgagcgcgtg ctcctgtagc tgcgccggat cgaccgcgcc
     1021 cgcgttcttc gccgtgaacg cgaacggcaa attctcgccc gtcgcgagcg cgctctgaag
     1081 gtagaagctc tcgccgcgcc cgcgcaccgc gctgtcgata atcaaagcct tgcccggctt
     1141 ctcgccgcgc agcgtgaagt agaactggtt gtcgtacgcg aagtcttcgg cgcggacgga
     1201 gacgacgcag cggttcgggc cttcggcgag gttgaagtcc gtgaactcga cgaccttcgc
     1261 ctcgcgcggc gcgagcgcga cctcgcgctt ctcgaccgtc tgctcgttga gctggaagtc
     1321 gattgtcagc cgctcgcgcg cctcgtccga aaagttggtc acgtgcaccg ccagcttgtc
     1381 ggcgtacttc gcgccgtaga ccgtgccgcg cgcctcgacg ttcacgaccg cgacgttggc
     1441 cgccgcgtcc gcgccgccca cgtcgatcgg caccagccgc acgtcgtagc tcaacttgaa
     1501 agtggacttc gccgcgtccc accccgtcgc ctgaaaatcc gagacgagga agacgcgctt
     1561 gaggcccgcc gtcttcaacg cgccgagcag cgcctcggcc ccgcgcaaag cctgctcgta
     1621 gtccgtcgcc tcgtaaccga cctccagccc cgcgagcgcc gcgtgcgcga ccttcttgtc
     1681 ggcggtgaac gggccgaccg cttcgtaacg ctcgccgaag gcgacgaccg ccacgcgctc
     1741 gtccgcctgc gcctcgccga tgatcgcctc cgcgcgccgc ttggcctccg cgaagtgttc
     1801 gccctgccgc atgctcaaag aagaatcgac gaggacgacg ctggcgcgcg tctcctcggt
     1861 ctccttcgcc gcgcccgcgc cgctgaagaa cgggcgcgtg aaggcgaaga ccaggagcag
     1921 caccgcgagg caccgcagga tgagcagcag caggttgtgc agcgtccgcc ggcggatggt
     1981 gcgctgcgga acctgccgca cgaagtagag cgccgggaac tcgacgcgcc gcgcccgcgt
     2041 gcgccgcacc agatgcacca gtatcggcgc ggcgatggcc gcgaggccga gcaggaataa
     2101 tggattcagg agcgacatct ttcaaaggcc gtaagccgtg aaccggaaac cgtgacaagt
     2161 gaagaccggt tcttacttgt cgcggttcac ggtttacctg tcacggcttt ccctcagcac
     2221 tcatcacttt tgcttccccg cgcgccgcga caggaaggag aagagcgcga agtcgagcgg
     2281 ctgatcggtc gtgatcatct cgtaatcgac gtggttggcg gcgcagcagc ggcgcatctc
     2341 ttcgacatgc gcggcgatgc gctcgcggta gcctttcgcc accacgtcgg ggagcaccgg
     2401 cagctgctcc gccgtctcgg catcttccaa gaggagcacc gcgtcgtcga agtcgaactc
     2461 gacctcgttc cggtcgagga cgtggaaggc gatgatgtcg tgccctttga agcgcaggtg
     2521 gcggaaggcg tcggagagcc gctcgggctg gtcgtagaag tccgagacga ggcagaccag
     2581 accgcggcgc gcgagccgct ccgccgtctc gtgcagggct tccgcgaggc gcgtctcgcc
     2641 gcccgcttcg agcgccgcga gctgcccgta gatcgtgcgc atgtggcccg tccgattgcg
     2701 cgcagggacg tgcgtgtgaa ccttgtcgcc aaagccgacg aggcccaccg cgtcctgctg
     2761 ccgcgcgacg aggtgcgcga gcgcggcggc aaggaactgc gcgtactgga gcttcgtcac
     2821 gcgaccgtcg ctcgcgtagc gcatcgacgc gctcgtgtcc accagcaggt gacactgcgt
     2881 gttcgtgtcg gcgcggtact tcttgatgaa gtagcggtcg gtgcgcccca gcagccgcca
     2941 gtcgaggtag cgcaaatcgt cgccggggtt gtactgcctg tactcggtga actcggtcga
     3001 gaagcccgtg aacggcgagc ggtgcaggcc gaggatgaac ccttcgacga cggcgcgcgc
     3061 cagcagctcc agcgaaccga tgcgcgccag cacctccggg tcgaggaacc gcgccgggcc
     3121 gggcgcagcg ctcggcgccg ccgccgcgct ctcgtcggcg accgcctccg ccgcactctt
     3181 cctgtttcgc ctccaccaca taagaacgag tgatgaggac tgaggactga ggactgagta
     3241 aaaagctatt ttttatttaa ctcagtcctc agtcctcagt cctttgtttc aagtctttgc
     3301 gcatcggcac gaactcgatc ttgacgccgc cgccagcgtc gtactcgacg cgctcgtcgc
     3361 cttcgtagcc gcgcgcggcg tagagcctga cgccgggcag cgtggccatc agttcgagcg
     3421 cccggaagcc gtgctcgctc gcctcgcgct cgcaggtttc aagaatggcg cggcccacgc
     3481 cgcgtctcgc ccacgcgggg tggacgaaga aggcgcggat ttttgccggc tcggcggcgg
     3541 ggtcgagttc gcccgaatcc ctcgcggcgt agcggtcgcc gccgaagagc gtccttcgcc
     3601 tgctccaccc gccgcagccg acgagccgcc cttcggcctc ggcgacgaag tacgtcccgt
     3661 ccagaatcaa gtcggtgtcc acgccgaaga cagacgtgat ggccgcctcg atctgctcgt
     3721 ccgtataatc cgcccgactc aaagaccgcg ccgactcggc gatcagctct tcgatggcgt
     3781 cacgctcgtc gagggtcgct tttctgatga cgtaatccac acgccccgtc acagcccgga
     3841 cttcggctcg ggcacggcct cgatcagttt tttgacgacc gcgtcggagt cgatcttctc
     3901 ggcctcggcc tggaagttca gcagcacacg gtggcgcagg acggcgggcg cgacggcgcg
     3961 cacgtcctcg cacgagacgt tgtagcgacc gcgcagcacg gcgcgcgcct tgccgccgag
     4021 gatgaggaat tgcgaggcgc gcaagctcgc gccccacgtc acccagcggt tgacgaagtc
     4081 gggggcggcg ggcgtccccg gacggctcgc gccgacgagg cgcaccgcgt agcgagccac
     4141 ggtgtcggac gcgggcacct gccggacgag gcgctggaag gcgatgatct cttcggcgct
     4201 catctgcggc tcgaactcgt aatcctgcgg cgcggtggtc tgcttgacca cttcgacctc
     4261 gtcctcttca gggagatacc cgaccctgat ctggaacatg aagcggtcga gctgggcctc
     4321 gggcagcggg taggtgccct ccatctcgat ggggttctgc gtggcgagga cgtggaaggg
     4381 ccgcggcaac tcgtaactca cgccctgcac ggtgacgcgc ccctcctgca tcgcttccag
     4441 gagcgcggcc tgcgtcttcg gcggcgtgcg gttgatctcg tcggccagca cgatgttggc
     4501 gaagacgggg ccgcggatga actccatcgc gcgccttccg gtggcgcggt cttcctcgat
     4561 gatctccgtg ccggtgatgt cggcgggcat caggtcgggg gtgaactgga tgcgcttgaa
     4621 ggagaggtcg agcacggagg agacggtgcg gacgagcagc gtcttggcga ggccgggcac
     4681 gcctgtcagg atcgagtggc cgcccgcgaa cagcgacagg agcacctgct ccaccacgtc
     4741 gtcctgcccc ttgatcgcct tgcggatctc ccgcagcagc ttctcgcgcg ccgacgcgag
     4801 ccgatccaac agctcctcgt cgcgcatcac agtcgtctcg acggccatgc tctctcctta
     4861 gaatacaacg tactgaggac tgaggactga gtaaaggcgt tgtcttcttc ctcagtactc
     4921 agcactcagt actcatcact tccgttcact gccccgtgat cgtcaacagc agctcctgcg
     4981 ccttctcgta gctcggcgcg gcttcgagcg cgcgcagcac ggagcgcttg gcgtccgcct
     5041 gtttgccgag cgcgtggaag gcgcgcgcga cgttgtagtt ggcctcggcc tcgttgggcg
     5101 gctgcaaggc gagcgccgcc tgaaactcgc gcagggcggc agccgcgtcc gcgcgcgcga
     5161 gcagtatctc tccggcgcgc gcgtgcggcg cgtgctcgaa ggggctgacg tagaaactga
     5221 ggcgcagcgc gtcgagcgcg cgcgccgcgt cgttctgctt gagccgcaac tcggcgagcc
     5281 gcttcagcgc gtcgaggtta ttctcgtcga gtttgacgag cagttccaga gcgagcgccg
     5341 cctcgccggt gtcgccctgc ttctcgaaga tgtcggccaa agactcgtag gcgttgcccg
     5401 gccccgtgta gtaagggaac agctcggcgc cgcgtttgta gtggcgggcc gcgctcgccg
     5461 cgtcgccctc ggcctgatac aacgcccccg cgcgcaggtg gagcgcgaag gtctcctgcg
     5521 tgccgaggcg tttcagcact tcgtccttcg gcagttgcgc cacctctgcc ccggcggtgc
     5581 ccatcgccag ttgcagcggt cgcgccttcg cttcgatgta ctcggcgaag gcgcggtcga
     5641 actcggcctc gctgagcttc aaagcctgcc gcagcacctc gggcgtgcgc gccttgtcgc
     5701 ggtagagcgc gagcatcttc aggatcgcgt cgaagccgtg gcgctcgacg acgaactcga
     5761 cgacctgcga cgcctgaaag taggcgagcg agatgtcgtc cgggcgcttc gggcgcatga
     5821 accctgagtc gaggtctgcg atcttgaacc agcgaccgtc ggcgtgcgcg cgcaggacgg
     5881 atgcgttcca gtcgtcgccc caaccgcgcc gcgcgcgccg ctcctcgtaa accgagatgc
     5941 cttccgagaa ccagcgcggg atgcggtagt cggtcatctg caaagtgatg acgtgcgcgt
     6001 actcgtgcca caacgtgctg ccccagttga actctccggc gggccgcgcc gaaggcgagt
     6061 cctgcgcgat gaccgggccg aagcagacgc cgagcgcgcc gagtccgggc agacccaggg
     6121 cgcgcaccgc gaagtcttcg tggttcggga acagctcgac gtagaccggc ccctgcggcg
     6181 tgaagcggta cttcgccgtg agcttcgcag cggcctcttc caaaaggtct gccgcgtacg
     6241 ggccgagcac gtcgctctcc gtcgccgccg ccttgacgac gaactggccg cgcttcgtct
     6301 cgcggtattc gctcatcgtg tccagaaggt cgagcgtgtt cttcgcccac acgttgaacg
     6361 ggtcgcccgc gaaagacttc tcgacggcgg ctctgccctc ctccatccgg
//
LOCUS       DBNGOH010000215         4551 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2048211, whole genome shotgun sequence.
ACCESSION   DBNGOH010000215 DBNGOH010000000
VERSION     DBNGOH010000215.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4551)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4551)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4551
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2048211"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..627)
                     /locus_tag="AC159P_04100"
     CDS             complement(<1..627)
                     /locus_tag="AC159P_04100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694667.1"
                     /translation="MYDTNGTRSVCQPAHTDYDIAARTNWLKAEIARKERTRLFARRE
                     REEQQAMRLLDPVPAREAFARFGLFLGLLPPAAIFGRVLMESHDEQSFAMFVAFAVAM
                     NAVCCLVGRAMARSLAKGAEKIERRAWWQMLFLPALLGSVWGLVTGSSGGVLVFGLGA
                     FVGAACAVPVGALAFSVFMLLHRLLARSGMIEERQLWPLAFGIPSVIAA"
     gene            complement(684..1538)
                     /locus_tag="AC159P_04105"
     CDS             complement(684..1538)
                     /locus_tag="AC159P_04105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694668.1"
                     /translation="MRLSEFITIYLAAAAPFGVAHFLRCETRGGHHRARALTRALVAA
                     LLWPLAAFVMFGKSKDESQDEAGERASDDERKIERTKRALVGALRDVEEILEPMRGPE
                     GETERHALFAAREAVERYVGLTLAARGLDADAAPSAREMELCRIAGRTGDDLSNAGRC
                     VHRRNVSRLHAHQERARAELFHALAATRETATEHHAASAPPARARAAEAMIRAFARAI
                     ELLSLLEDRAAVLRVARLLDAECAHLRRLETAGAALGAAEVNNTHVNVQAPHFGVTAA
                     GEGLKAGL"
     gene            2147..2377
                     /locus_tag="AC159P_04110"
     CDS             2147..2377
                     /locus_tag="AC159P_04110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694669.1"
                     /translation="MKAKTPIKIIKRDERIRQEKPAEDAATNRKTPQEAARDIVSTVS
                     GWVNELQHKRRVETARAIKTLFNDATPQPSKA"
     gene            2721..2894
                     /locus_tag="AC159P_04115"
     CDS             2721..2894
                     /locus_tag="AC159P_04115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694670.1"
                     /translation="MKSAKKQSAKREARPVFDSIRKPLAPPGHPLSRAKPEERAHPAE
                     RKVKHKRRPDNSE"
     gene            2990..3514
                     /locus_tag="AC159P_04120"
     CDS             2990..3514
                     /locus_tag="AC159P_04120"
                     /inference="COORDINATES: protein motif:HMM:NF019241.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HIR3694671.1"
                     /translation="MNEVARAFIEEARSQLSAEYLPKIERCLERLGDEQVWWRPNEQS
                     NSIGNLLLHLEGNARQWVVCGVGGAPDRRARDAEFDERERIPRADLLARLRATLSEID
                     GVLARLDPATLLERRRIQRLDVTLLRAVFHVVEHFSMHTGQIIQLTKLLTGGDLAFYD
                     FSAGAPRANWNEKR"
     gene            3847..4122
                     /locus_tag="AC159P_04125"
     CDS             3847..4122
                     /locus_tag="AC159P_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010587349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HIR3694672.1"
                     /translation="MTYVVCEPCVGCRYTDCVVVCPVEAFHLDDAAVWINPETCIDCD
                     ACVPECPVEAIFAEDKVPDEWQHYTAINAEKSLSGLPIVSEKLDPLC"
     gene            4240..>4551
                     /locus_tag="AC159P_04130"
     CDS             4240..>4551
                     /locus_tag="AC159P_04130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694673.1"
                     /translation="MNTKKKTAETKRRGRRWLGALAGLTAGVLGTALLMRRGRRVPGK
                     KSIYEQYNRGEADRWARPGMSVTFRAELMPGRSRAERTFRVAGVLASGRITLEDFAGE
                     HA"
BASE COUNT          808 a         1512 c         1541 g          690 t
ORIGIN      
        1 ggcggcgatg acggacggga tgccgaacgc gagcggccag agttggcgct cctcaatcat
       61 accactgcgg gcgagcaggc ggtgaagcag catgaagacc gagaaggcga gtgcgccgac
      121 cgggacggcg caggccgcgc cgacgaaggc gccgaggccg aagacgagca cgccgcccga
      181 cgagccggtg acgagtcccc agacggagcc gagcagcgcg ggcaggaaga gcatctgcca
      241 ccacgcgcgc cgctcgatct tctcggcgcc tttggcgagc gaccgcgcca tcgcgcgccc
      301 gacgagacag cagaccgcgt tcatcgccac ggcgaacgcg acgaacatgg cgaaggattg
      361 ctcgtcgtgc gactccatga gcacacgacc gaagatggcc gcgggcggga gcagaccgag
      421 gaagaggccg aaccgcgcga acgcctcgcg cgccgggaca gggtcgagca ggcgcatcgc
      481 ctgctgctcc tcgcgctcgc gccgcgcgaa gaggcgcgtc cgctctttgc gcgcgatctc
      541 ggccttcagc cagttcgtcc gcgcggcgat gtcgtagtcc gtgtgtgcgg gttggcagac
      601 gcttcgtgtg ccgttggtgt cgtacatatt gcgcctcctc cgggcgcgat tcacgtacat
      661 ggtgtgcctc ctccgggcac gtttcagagt cccgccttga ggccctcgcc cgcggcggtg
      721 acgccgaagt gcggcgcctg aacgttgacg tgcgtgttgt tgacctccgc ggcgccgagg
      781 gccgcgcccg ccgtttcgag ccggcgcagg tgcgcgcact ccgcgtcgag caggcgcgcg
      841 acgcgcagca cggcggcgcg gtcttcgagg agcgagagca gttcgatggc gcgcgcgaac
      901 gcacggatca tggcctccgc ggcgcgcgcg cgcgccgggg gcgccgatgc cgcgtgatgt
      961 tcggtcgccg tctcgcgtgt cgccgcgagg gcgtggaaga gttcggcgcg cgcacgctct
     1021 tggtgcgcgt gcagccgcga cacgttgcgc cggtggacgc agcggcccgc gttcgaaagg
     1081 tcgtcgcccg tgcggcccgc gatgcggcac aactccatct cgcgtgcgga tggcgcggcg
     1141 tcggcgtcga ggccgcgcgc cgcgagcgtc aggccgacgt accgctcgac cgcttcgcgc
     1201 gcggcgaaca gcgcgtgccg ctccgtctca ccttcggggc cgcgcatggg ttccagtatc
     1261 tcttccacgt cgcggagcgc acccacgagc gcgcgcttcg tccgctcgat ctttcgttcg
     1321 tcgtccgagg cgcgttcgcc cgcctcatcc tgactttcgt ctttcgactt tccgaacatg
     1381 acgaaggccg ccagcggcca gaggagcgcg gccaccaggg ctctcgtcaa cgcgcgcgca
     1441 cggtggtggc cgccgcgcgt ctcgcagcgc aggaagtgtg cgacgccgaa gggggccgcc
     1501 gccgcgaggt agatggtgat gaactcgctc agtctcatcg cgcacggctc aagggcaacg
     1561 gacatgccag cgtccgcgcg ccgcgccgcg cgtgaagacg ccgaaggtgt cttccgctgg
     1621 aacgccgtgt attacgcggg tgcggcgagg ggggcgcgtg caagtcgcga gaggcgccgc
     1681 cgttcatcct ttggcaaagg gcatgagcgg cgggccggat tggcaaagtg caaaggaata
     1741 aaatccgcgc gccgcgggct gtacgtattg aagccacgtg cgagtgaaag tgtccgaggc
     1801 cactgcgcga agagagggag gtgaacggct tgagggcgca cgcgagttca caggcaacac
     1861 attcggcgcg gcgcgcatct cttcttcgct ccctatcttt cccgctgcat tcctcgcaag
     1921 tgtgtggcga cggcccgccg ggatgtcaaa gatttgttgc gatgtcttta cgacgcactt
     1981 ggagggccgt gctctgcgcg ccggctgcgt cgcgtggcct ttgaattgca cttcgacctt
     2041 gcaacgccca cggcggcaga cgggaaccga acaaattgga acttttggac agcctccatc
     2101 gtctaagagt gaacgaaagt cgatacgaac aagcgaaaca gtcatcatga aagcgaagac
     2161 cccgatcaag atcatcaagc gtgacgaaag aattcgtcag gagaagcccg cggaagacgc
     2221 ggccaccaac cgcaagaccc cgcaagaggc cgcgcgtgac atcgtctcta ctgtcagcgg
     2281 ctgggtgaat gagttacagc ataagcgccg cgtcgagacc gcgcgcgcca tcaagaccct
     2341 gttcaacgac gcgacaccgc agccgagcaa ggcatagagt tctcggtttc caccaaggcg
     2401 aaggggacac aagggcacaa cgagcgacgg gcaacgagcg cgaccgggag gctccatccg
     2461 agcttcccgg tcgcgctctt ttcggtcggc ggtctgctcg ggccgtccga cggaatcaac
     2521 cgcgaaggca taccggacac aacggaggcc gaagacgatg aagaagacgt tgcttcgagt
     2581 cttacgtttt catcttcgcg ccttcgtggt tgtgtcagtc gttctcatcc acccgtctcc
     2641 gtccatgagt gttgacacga acgcggcggc gcgcgtagca tcccggccat cacagcgaaa
     2701 actcgcaggg ggcttcgaag atgaagagcg ccaagaaaca gtccgccaag agggaagcgc
     2761 gtcccgtgtt cgactccatc cgcaaacccc tggccccgcc gggccacccg ctgagccgcg
     2821 ccaaaccgga agagcgcgca caccccgccg agcgcaaggt caagcacaaa cgccgccccg
     2881 acaattcgga gtaatgaaca aggatgcggg cggagcgacg gcttgacgcg cgggcatccg
     2941 ccgccgcgtc ttgctaccac cctgcccgtg gccggaagga gccgcagacg tgaacgaggt
     3001 ggcacgagct ttcatcgagg aggcgcggtc gcaactctcc gccgagtacc tgccgaagat
     3061 cgagcggtgc cttgagcgcc tcggcgacga gcaggtctgg tggcgcccga acgagcagtc
     3121 gaacagcatc ggcaacctgc tgctgcacct cgaaggcaac gcgcgccagt gggtcgtgtg
     3181 cggcgtcggc ggcgcccccg accgccgcgc gcgcgacgcc gagttcgacg agcgcgaacg
     3241 aatcccgcgc gccgacctgc tcgcgcggct ccgcgccacg ctctccgaga tcgacggagt
     3301 gctcgcgcgg ctcgaccccg cgacgctcct tgagcggagg cgcatccagc gcctcgacgt
     3361 gacgctcttg cgcgccgtct tccacgtcgt cgagcacttc tccatgcaca ccggccagat
     3421 catccaactg accaagctgc tcacgggcgg cgacctcgcc ttctacgact tttcggctgg
     3481 cgcgccccgc gcgaactgga acgaaaagcg gtaggcagca ggcagcgggc agtaggcagt
     3541 gaaagccaga ggcttgagag ctaacgtctc aagcctctgg cttttttgct tgaagcgcgg
     3601 agaggctcag gactttacct ctcaagcctc tccgcccttc tgtattgact gcctgctgcc
     3661 tactgcctac tgcctacttt tttcactttt cctctttcaa aactcgcggg ttcgtgctta
     3721 atatatccgc cggagtaaac atcccgcggt catgtcggcg cggggccgca agtcgcccgc
     3781 acggcccaag gactgaggcc ccgcgccgcc gtcgtcatac acacctttga ggaggctgga
     3841 atctgaatga cctacgtggt ttgcgaaccg tgtgtcggct gccgttacac ggactgtgtg
     3901 gtggtctgcc ccgtcgaggc tttccacttg gatgatgcgg cagtgtggat caaccccgag
     3961 acgtgcatcg actgcgacgc gtgcgtgccc gagtgcccgg tcgaggccat cttcgccgaa
     4021 gacaaggtgc ccgacgagtg gcagcactac acggccatca acgccgagaa gtcgctgagc
     4081 gggctgccca tcgtcagcga gaagctcgac ccgctctgct gacgccggca gaccacggcg
     4141 cgcccgctcg tcaggcgcgc gagacagacg ggcgcgcaag cacgccgaac gcttccgagg
     4201 ggcgtgcgct tgcgcgcccg acgcgcggac gcggacggag tgaacacgaa gaagaagacg
     4261 gcggagacga agaggcgcgg gcggcgctgg ctcggcgcgc tggcggggct gaccgcgggg
     4321 gtgctgggga cggcgctgct catgcggcgg gggaggcggg tgccgggaaa gaaatccatc
     4381 tacgagcagt acaaccgcgg cgaggccgac cgctgggcgc gccccggcat gagcgtcacc
     4441 ttccgcgccg agctgatgcc gggccgcagc cgcgccgagc gcaccttccg cgtcgccgga
     4501 gtcctcgcga gcggtcgcat cacgctcgaa gacttcgccg gcgagcacgc c
//
LOCUS       DBNGOH010000216         5154 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3882120, whole genome shotgun sequence.
ACCESSION   DBNGOH010000216 DBNGOH010000000
VERSION     DBNGOH010000216.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5154)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5154)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5154
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3882120"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..888)
                     /locus_tag="AC159P_04135"
     CDS             complement(<1..888)
                     /locus_tag="AC159P_04135"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3694674.1"
                     /translation="MAKNLVIVESPAKAKTINKYLGSDYRVLASFGHIKDLPKKGLGV
                     DLQNDFQPTYEINDEPRKKKVVAELKKAAKEAEVIYLAADPDREGEAICQHLAEEIVP
                     KRPKKPSFRVMFNEITKRAVQEAFEHPKQIDQNLVDAQQARRVLDRLVGYKVSPLLCR
                     TIGGKLSAGRVQSVALRMVVEREREIENFVKTEYWTIAANLSARLPPAFDARLYKIGE
                     QTVKTSGFDQEIRKNEILIGDEAQAKEIVAKHGAFDTVFLRHVVEHVSDLDGFFAGIR
                     SLLRDDGLLVLELPEVEESF"
     gene            complement(1295..2422)
                     /gene="dprA"
                     /locus_tag="AC159P_04140"
     CDS             complement(1295..2422)
                     /gene="dprA"
                     /locus_tag="AC159P_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HIR3694675.1"
                     /translation="MKDWIALNMTPGVGPRAAARLLERFGSAEGVFGALRSELERLRL
                     RPEAVESIALRDRHEEAARELERVRGMEGADVLVLDDGNYPALLREIADPPVTLYVRG
                     EWEAATEAPCVALVGSRRCSTYGQNVAAMLARELASRGVTVVSGLARGIDAAAHRGAL
                     DAGGRTVGVLGTGLDEVYPRDHAKLAAEMLGRGGALVSQFPLGTPPVPENFPYRNRII
                     SGLCLGVVVVEAAENSGSLITARLAMEQNREVYAVPGNITSRNSFGTNYLIKGAGAKL
                     IQTWQDIVAEFPPDLAARLLPPEPKKSARRADAVAALPEDISESARNVFRLIPADEPA
                     HIDALAAATGMPLPELSAALLELEMRELIRQLPGKCFVRKL"
     gene            complement(2542..2640)
                     /locus_tag="AC159P_04145"
     tRNA            complement(2542..2640)
                     /locus_tag="AC159P_04145"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2604..2606),aa:Sec,seq:tca)
     gene            2789..4015
                     /locus_tag="AC159P_04150"
     CDS             2789..4015
                     /locus_tag="AC159P_04150"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3694676.1"
                     /translation="MSASGATATGERDAASQSSRGARTAAYFAAMAGLGLTLGAFGPT
                     LPGLAARTGVGLGDIGVLFVARSLGFLLVSVRGGRLYDRVPGHRVMAAMLLLMAAAMA
                     LVPAVPRLWPLAVVVAVLGAAEGTLDVGANTLLARTHGARVGPYMNGLHFFYGVGAFA
                     APLVVARLVAHAHGLALTYWSLALLLLPVAAWLARLPSPPVRVPTGEEDEGRVGRRTI
                     VLVALFFFLYVGAEVTFGGWVYSYAVALRLGAETSAAYLTSAFWGALTLGRLAAVPLA
                     ARFSPRALLAADLAGCFCSLALILSRPDSAAAFAVGTVALGLSMASVFPTMFALAGGR
                     VVLTGRVAGWLIVGASAGSMFLPWLAGRLFDAAGPRSMPVAVACALALAACVFAALAR
                     TRVGGIKPAPRGMKDA"
     gene            4037..4633
                     /locus_tag="AC159P_04155"
     CDS             4037..4633
                     /locus_tag="AC159P_04155"
                     /inference="COORDINATES: protein motif:HMM:NF017970.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HIR3694677.1"
                     /translation="MGETIHGNGAGDTPTRDADAASASAGAAESIRCPACGGANAPEA
                     VFCANAECGKALGPFAYVREELEQRTRWHERLADRVTAFIGRPQFILAHTLWFAAWLA
                     VNTGIVAFVARFDDYPYSLLGLIISVEAIFITGFLLISQNRQNAHADKRAELDYEVNV
                     RTYREISELKALLRELHERLDTMEGGGARGQSSERSGS"
     gene            4630..5139
                     /locus_tag="AC159P_04160"
     CDS             4630..5139
                     /locus_tag="AC159P_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR3694678.1"
                     /translation="MKRTGRLAAAITLLWLPSLGAVAARAQEPSAPAAATLKSHIMLT
                     PEEVKWGECPPALPAGAKCAAIEGDLGAANALFGYRVKMPDNYRIPPHFHPADEHVVV
                     LSGVFNMGHGDKLDTGATRALAAGSFAVMPKGAHHFAWTKGETVVHVYAVGPWGLTYV
                     NPKDDPRNR"
BASE COUNT          728 a         1815 c         1763 g          848 t
ORIGIN      
        1 gaagctctcc tcgacttcgg gcagttcgag gaccagcaag ccgtcgtccc gcagcagcga
       61 ccggatgccg gcgaagaaac catcgaggtc gctgacatgc tccaccacat ggcgcaggaa
      121 caccgtgtcg aacgcgccgt gcttggcgac gatctctttc gcctgcgcct cgtcgccgat
      181 caggatttcg ttcttcctga tctcctgatc gaagcccgag gtcttgacgg tctgctcgcc
      241 gattttatag agccgcgcgt cgaacgcagg gggcagccgt gccgagaggt tggcggcaat
      301 ggtccagtac tcggtcttga cgaagttctc gatctcgcgc tcgcgctcga ccaccatgcg
      361 gagcgcgacc gactgcacgc gcccggcgga gagcttcccg ccgatggtgc ggcacagcag
      421 cggcgagacc ttgtagccca ccagacggtc gagcacgcgc cgcgcctgct gcgcgtccac
      481 gaggttctgg tcgatctgct tcgggtgctc gaacgcctcc tgcacggcgc gcttcgtgat
      541 ctcgttgaac atcacgcgga acgagggctt cttcgggcgc ttcggcacga tctcttcggc
      601 gaggtgctgg cagatggcct cgccctcgcg gtcggggtcg gcggccagat agatgacctc
      661 ggcctccttc gccgccttct tcagctcggc gacgaccttc ttcttgcgcg gctcgtcgtt
      721 gatctcgtag gtcggctgaa agtcgttctg gagatcgacg ccgaggccct tcttcggcaa
      781 gtccttgatg tggccgaacg acgccagcac gcggtaatcc gagccgagat atttattgat
      841 cgtcttcgcc ttcgcgggcg actcgacgat gactaaattc ttcgccatta cgttcccttc
      901 gtctcaaaaa caacgcgcgg cttgccggct tcacgctcgc gccgcgcccc tcgtataaac
      961 tgtttctgac gccttgtaaa acctctccct aataaactcc attttgctct tagatgcaaa
     1021 ccggcgcccg cgggcttgcc cgccgccgcc acttatccgc cttgaatttc aaagacttac
     1081 ggccaaaccg tggtccccga acggcctccg cgacggctcg ccggccccgt ctgcggcggg
     1141 acgtttgttg ctcccccgcg ccttttcgtt gtcgccccgg cggggcttga agtgcgacgc
     1201 gagccgcccc gccgggtctc ttcagcgacc gtcggcgcgc atccgacgac accacgctcc
     1261 gaccgcgaac ggttctccga acgacctccc gcgctcacaa tttcctgacg aaacacttgc
     1321 ccggaagctg ccggatcagc tcgcgcattt cgagttcgag gagcgcggcg gagagttccg
     1381 gcagcggcat gcctgtggcc gcggcgagcg cgtcgatgtg tgcgggttcg tccgcgggga
     1441 tgaggcggaa gacgttgcgg gcgctctcgg aaatgtcctc gggcagcgcg gccacggcgt
     1501 cggcgcggcg cgcggacttc ttcggctcgg gcggcaaaag ccgcgcggcg aggtcgggcg
     1561 ggaactcggc gacgatgtcc tgccacgtct gtatcagctt cgcgcccgcg cccttgatga
     1621 gatagttggt gccgaacgag ttgcgcgagg tgatgttgcc gggcacggcg tagacctccc
     1681 ggttctgctc catcgcgagg cgcgccgtga tgagagagcc gctgttctcc gccgcctcga
     1741 ccacgaccac gccgaggcag agaccgctga tgatgcggtt gcggtaggga aagttttccg
     1801 gcacgggcgg cgtccccagc gggaactgcg agacgagcgc gccgccgcgc ccgagcatct
     1861 ccgcggcgag cttcgcgtgg tcgcgcgggt agacttcatc cagccctgtg ccgagcacgc
     1921 cgacggtgcg cccgcccgca tcgagcgcgc cgcggtgcgc cgccgcgtcg atgccgcgcg
     1981 cgagccccga gacgaccgtc acgccgcgcg acgccagctc gcgcgccagc atcgcggcca
     2041 cgttctgccc gtacgtcgaa caacgccgcg agccgaccag cgccacgcac ggcgcctccg
     2101 tcgccgcctc ccactcgccg cgcacgtaga gcgtgacggg cgggtctgcg atctcgcgca
     2161 ggagcgcggg gtagttgccg tcgtccagga cgagcacgtc cgcgccctcc atcccgcgca
     2221 cgcgctccag ctcgcgcgcg gcctcctcgt gccggtcgcg cagcgcgatg ctctcgacgg
     2281 cctcgggccg cagacgcagc cgctccagct ccgacctgag cgccccgaac accccttccg
     2341 ccgacccgaa gcgctccaac agacgcgccg ccgcgcgcgg ccccacgccc ggcgtcatgt
     2401 tcaacgcgat ccaatccttc atagtaagca gtgagcggtg agcagtgagc aggggaaagc
     2461 gggcggcggg caatgagcag taagcacgaa aagcagttcc tggaactgct cactgctcac
     2521 cgctcactgc ttactttctt gtggcggagg cgtgtgggaa tcgaacccac ccgcgcctgc
     2581 tctcacaggc gcgctgacgg ttttgaagac cgcgccgttc accagaacag atgcgcctcc
     2641 gcgtgtgtgg ccagggcgac caacgtacgc ggcggcggcg cccgctgtca agcaaggcgt
     2701 cgggagcgag aacttggccg gcagcgtggt gagtcggcat aatcggagca tggttgcgga
     2761 ttcggaaaaa gtttcggatg tgccgacgat gagcgcgagc ggcgcgacgg cgacagggga
     2821 gcgcgacgcg gcgagccagt cttcgcgcgg ggcgcgcaca gccgcctact tcgccgcgat
     2881 ggcgggactg ggcctgacgc tcggcgcgtt cggtccgacg ctgccgggac tggcggcgcg
     2941 cacgggcgtc ggcctcggcg acatcggcgt gctcttcgtc gcgcgctcgc tcgggttcct
     3001 gctcgtctcg gtgcgcggcg gtcggctgta cgaccgcgtg ccgggccacc gcgtgatggc
     3061 cgcgatgctc ctgttgatgg cggcggcgat ggcgctcgtc cccgcggtgc cgcggctgtg
     3121 gccgctggcc gtcgtggtcg cggtgctcgg cgccgcggag gggacgctgg acgtgggggc
     3181 gaacacgctg ctcgcgcgca cgcacggcgc gcgggtcggg ccgtacatga acgggttgca
     3241 cttcttctac ggcgtggggg ccttcgcggc gccgctcgtc gtcgcgcgcc tcgtcgcgca
     3301 cgcgcacggc ctcgcgctca cctactggtc gctcgcgctg ctgctcctgc ccgtcgccgc
     3361 gtggctcgcg cggctgccga gtccgccggt gcgcgtcccg acgggcgaag aagatgaggg
     3421 gcgcgtcgga cggcggacaa tcgtgctggt cgcgctcttc ttcttcctct atgtcggcgc
     3481 ggaggtgacg ttcggcgggt gggtctattc gtacgcggtc gccttgcggc tgggcgcgga
     3541 gacctcggcg gcctatctca cgtccgcgtt ctggggcgcg ctgacgctgg ggcggctcgc
     3601 cgccgtcccg ctcgccgcgc gcttcagccc gcgcgcgctg ctcgccgcag acctcgcggg
     3661 ctgtttctgt agcctcgcgc tcatcctctc gcgcccggac tccgcggcgg cgttcgccgt
     3721 cggcacggtc gcgctcggcc tctcgatggc ctcggtcttc ccgacgatgt tcgcgctcgc
     3781 gggcgggcgc gtcgtgctga cggggcgggt cgcgggctgg ctcatcgtcg gcgcgagcgc
     3841 ggggagcatg ttcctgccgt ggctcgccgg tcgcctgttc gacgcggcgg ggccgcgctc
     3901 gatgcccgtc gcggtcgcct gcgccctcgc gctcgcggcg tgcgtcttcg ccgcgctcgc
     3961 acgcacgcgc gtcggcggaa taaaacccgc gccgcgcggg atgaaagatg cgtgaggctt
     4021 tgagggcgac ctccacatgg gcgaaacgat tcacggcaac ggcgcgggcg acacgccgac
     4081 gagagacgcg gacgccgcgt ctgcgtccgc gggcgcggcg gagtccatcc gctgccctgc
     4141 gtgcggcggc gcgaacgcgc cggaagcggt cttctgcgcg aacgccgaat gcggcaaggc
     4201 gctcgggcct ttcgcctacg tccgcgaaga gcttgagcag cgcacgcgct ggcacgagcg
     4261 gctggccgac cgggtgaccg cgttcatcgg caggccgcag ttcatcctcg cccacacgct
     4321 ctggttcgcg gcgtggctcg ccgtcaacac gggcatcgtc gccttcgtcg ccaggttcga
     4381 cgactacccc tacagcctcc tcggactcat catctcggtc gaggccatct tcatcacggg
     4441 cttcctgctc atcagccaga accgccagaa cgcgcacgcc gacaagcgcg ccgagctgga
     4501 ctacgaagtc aacgtccgca cctaccgcga gatcagcgag ctgaaagccc tcctgcgcga
     4561 gctgcacgag cggctggaca cgatggaggg cggcggggcg cggggtcaat caagtgaaag
     4621 gagcgggtca tgaagagaac gggccggtta gccgcggcca tcactctgct ctggctcccg
     4681 tcgctcggcg cggtcgccgc gcgggcgcag gagccgtccg cccctgcggc ggcgacgctc
     4741 aagtcgcaca tcatgctcac gccggaggag gtcaagtggg gcgagtgtcc gcccgcgctg
     4801 cccgccgggg ccaagtgcgc ggcgatagag ggcgacctcg gggccgcgaa cgcgctgttc
     4861 ggctaccgcg tcaagatgcc ggacaactac aggattccgc cgcacttcca cccggcggac
     4921 gagcacgtcg tcgtgctctc cggcgtcttc aacatgggtc acggggacaa gctggacacg
     4981 ggcgccaccc gcgccctcgc ggcgggcagc ttcgccgtca tgccgaaagg cgcgcaccac
     5041 ttcgcctgga cgaaaggcga gacggtcgtc cacgtctacg ccgtcggccc ctggggcctc
     5101 acctatgtca acccgaagga cgacccgcgg aatcgttgac ggcgggccgc acgc
//
LOCUS       DBNGOH010000217         3565 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1498403, whole genome shotgun sequence.
ACCESSION   DBNGOH010000217 DBNGOH010000000
VERSION     DBNGOH010000217.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3565)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3565)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3565
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1498403"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            419..2530
                     /locus_tag="AC159P_04165"
     CDS             419..2530
                     /locus_tag="AC159P_04165"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008469143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HIR3694679.1"
                     /translation="MKRFRPITRLALALLVGLASSSPPPGARARAGGPAARFFPAKEL
                     MRVGVYYYPEHWPEGQWARDFANMERMGFEFVHMGEFAWAFMEPEEGRFDFAWLDRAV
                     ALAARHNLRVILCTPTPTPPAWLYEKYPEAYLVGADGRRREHGSRGNNALADPNYLRL
                     SERVVGALARRYGRHPAVWGWQLDNEPMAQPDYSPSAQAAFRGWLRARYKTVEVLNRE
                     WGAAFWSLRYNSFGQIIIPNPTTLYGVSPHALLDYRRFTADQTAKFLNRQAAVLRRHT
                     APSQWITTNYISNIGSSDPRRSDELDFISYTMYPVGGGRGLGEDGFRLGWQHGIAFAN
                     SFYKSIKGVTGVMELQPGQVNWANINPQPAPGAVRMWLWHAFAGGCSFACTYRYRQPL
                     YGSEQYHAGIVGTDGVTPSPGGLEYQQVAREIRGLRARYDPKAVPPPGYAARRTGILW
                     NHENLWNMEHQKQTALWDTWGHMFRYLEIIKSFGAPLDYVSEAQDFSAYPVLVAPAYQ
                     LIDEELVRKWARYAEGGGHLVLTARTGQKKRNGQLWEARWAAPIHQLVGAEVSFFDLL
                     LDDGRGEAATDSATHRWNVWADVMKPLAGTESLATYTNQFYAGHAAVTRRRLGRGSVT
                     YIGVETRDGRLEREVLKRVYDRAGIATEDYAAGVYVDWRDGFWVGVNYSSRPADIPVP
                     AGAEIVIGEQPLKPASVLVWR"
     gene            2566..>3565
                     /locus_tag="AC159P_04170"
     CDS             2566..>3565
                     /locus_tag="AC159P_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012845088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HIR3694680.1"
                     /translation="MKHEMLEGVRQTARLRVFALLLAGLLSACTSATAQVLNDPVDVG
                     QDFQRMEQVYFVGSRVTDFDPATGAGLLQWDRYVRGTTLSFNKIDVTLGRGKGTEFPG
                     TEYDQDPRLPFSITFVSPRTVRLRFNTRSAPAPDAPSLMLAGPVPADRSWKVERTDKE
                     ITYTSAEGRVRLVKDPWHVEFYDRAGRLLTRTQNVGDPHTYFTPVPFSFVRRASDLAR
                     RTAAAFQLAHDEKIFGTGESFTRLDKRGQRVNAWTRDGMGVQNEFMYKPVPFFLSSRG
                     YGMFVHTSAPVTFDFGKTFDQHNNIYTGDEQLDLFVFLGTPKEVLSEYTALTGRSPV"
BASE COUNT          621 a         1223 c         1165 g          556 t
ORIGIN      
        1 gccgccccgc tgccgatcac ctgccgaaca agtgaagacg cccgcgggga tatttcagcc
       61 cggacgtgcc tgtcactaaa tacttcttga cagcggttgc cgactaataa tagaaagctt
      121 ttgccaactc ttgcaacaaa gtatccgccg cggtctgcac gacggaagcc cttactcctg
      181 aagttatcga gtcaccttcc ggaaagtaga ccgcgcagag cgtcgccacc cgcgcgagca
      241 aaagatttac aagccggtcg gtaccccgcc gaggtctggt tcacaagctc cgccggagcg
      301 ggacgtgtta catacgctgc cccgacgggc taagaaggct cgggccgcgg tgggctgcga
      361 acattccgtc cccggcggga ttcttcaaac acttctcaac gggaacgcac gcagggtcat
      421 gaagagattt cgtcctatca cacggcttgc gctggcgctg ctcgtcggtc tcgcctcgtc
      481 gtcgccgccg cccggcgcgc gggcgcgggc gggagggcct gcggcgcgtt tcttcccggc
      541 gaaggagttg atgcgggtcg gcgtctacta ctacccggag cactggccgg aggggcagtg
      601 ggcgagggac ttcgccaaca tggagcggat gggcttcgag ttcgtgcaca tgggcgagtt
      661 cgcgtgggcc ttcatggagc cggaggaagg gcgcttcgac ttcgcgtggc tcgaccgcgc
      721 cgtcgcgctc gcggcgcgcc acaatctgcg cgtcatcctc tgcacgccga cgccgacgcc
      781 gcccgcgtgg ctctacgaga agtacccgga ggcgtacctc gtcggggccg acggtcgccg
      841 gcgcgagcac ggctcgcgcg gcaacaacgc cctcgcggac ccgaactacc tgcgcctgag
      901 cgagcgcgtc gtcggggcgc tcgcacgccg ctacgggcgc cacccggcgg tctggggctg
      961 gcagctcgat aacgagccga tggcgcagcc ggactacagc ccgtcggcgc aagctgcttt
     1021 ccgcgggtgg ctcagggcgc gctacaagac cgtggaggtg ctgaaccgcg agtggggcgc
     1081 ggctttctgg agcctgagat ataacagctt cgggcagatc atcatcccca acccgacgac
     1141 gctctacggg gtgagtccgc acgccctgct cgactaccga cgcttcaccg ccgaccagac
     1201 cgcgaagttc ctcaaccgac aggccgccgt cctcaggcgc cacacggcgc cctcgcagtg
     1261 gatcacgacc aactacatct cgaacatagg gagcagcgac ccgcgtcgct ccgacgagct
     1321 ggacttcatc tcctacacga tgtaccccgt cggaggcggg cgcggcctcg gcgaagacgg
     1381 cttccgtctg ggatggcagc acggcatcgc cttcgccaac tcgttctata aaagcatcaa
     1441 gggcgtgacg ggcgtgatgg agttgcagcc gggccaggtc aactgggcca acatcaaccc
     1501 gcagcccgcg cccggcgccg ttcggatgtg gctctggcac gccttcgcgg gcggctgctc
     1561 gttcgcctgc acctaccgct accgccagcc gctctacggc agtgagcagt atcacgccgg
     1621 catcgtcggc accgacggcg tgacgccctc gcccggaggt ctcgaatacc agcaggtcgc
     1681 gcgcgagata cgcgggcttc gcgcgaggta cgatccgaag gccgtgccgc cgccgggcta
     1741 cgcggcgcgg cgcacgggga ttctctggaa tcacgagaac ctctggaaca tggagcacca
     1801 gaagcagacg gcgctgtggg acacgtgggg ccacatgttc cgctaccttg agatcatcaa
     1861 gtcgttcggc gcgccgctcg actacgtctc cgaggcgcag gacttctcgg cctaccccgt
     1921 cctcgtcgcg cccgcatacc agctgatcga cgaagaactc gtgcgcaagt gggcgcgcta
     1981 cgccgaaggc ggcggtcatc tcgtgctcac cgcgcggacg ggtcagaaga agcgcaacgg
     2041 gcagctctgg gaggcgcgct gggccgcgcc gatccaccag ctcgtcggcg cggaggtctc
     2101 cttcttcgac ctgctgcttg acgacgggcg gggcgaggcg gcgacggact cggcgacgca
     2161 ccggtggaac gtctgggccg acgtgatgaa gccgctggcg gggacggaga gtctcgccac
     2221 ttacacaaac cagttctacg ccgggcacgc ggctgtcacc cgccgacgct taggccgcgg
     2281 cagcgtcacc tacatcggcg tcgagaccag agacggtcgc ctggaaaggg aagtgctcaa
     2341 gcgcgtctac gaccgcgcgg ggattgcgac ggaagactac gcggcgggcg tctacgtgga
     2401 ctggcgcgac ggcttctggg tcggcgtcaa ctactcgtcc cgaccggctg acattcccgt
     2461 gccggcgggg gccgaaatcg ttatcggcga acagccgttg aagcccgcga gcgtgctggt
     2521 ttggagataa caacagccgc cgcagcgcgg acggagggaa gagggttgaa acacgagatg
     2581 ctcgaaggcg tgcggcagac ggcccggctg cgggtcttcg ccttgctgtt ggcgggtctg
     2641 ctgtccgcgt gcacaagcgc gacggcgcag gtgctcaacg accccgtcga tgtcggccag
     2701 gacttccagc ggatggagca ggtctacttt gtggggagcc gggtcacgga cttcgacccg
     2761 gcgaccggcg cggggctgct tcagtgggac aggtacgtgc gcggcaccac gctctccttc
     2821 aacaagatcg acgtgacgct cggcaggggc aagggcacgg agttccccgg caccgagtac
     2881 gaccaagacc cgcggctccc tttctcgatc accttcgtca gcccgcgcac ggtgcgcctg
     2941 cgcttcaaca cgcgctcggc gcccgccccg gacgcgccct cgctgatgct cgccggcccc
     3001 gtgcccgcgg acaggtcgtg gaaagtcgag cggacggata aagagatcac ctacacgagc
     3061 gccgaagggc gcgtgcggct cgtcaaagac ccgtggcacg tcgagttcta cgaccgcgcg
     3121 ggccgtctcc tgacgcgcac gcagaacgtg ggcgacccgc acacgtactt cacgcccgtc
     3181 cctttctcct tcgtgcgccg cgcctccgac ctcgcgcgcc gcaccgccgc cgccttccag
     3241 ctcgcgcacg acgagaagat tttcggcacg ggcgaatcct tcacgcgcct cgacaagcgc
     3301 ggccagcgtg tcaacgcgtg gacgcgcgac ggcatgggcg tgcagaacga gttcatgtac
     3361 aagcccgtcc cgttcttcct cagtagccgc ggctacggca tgttcgtcca cacctcggcg
     3421 cccgtcacct tcgacttcgg caagaccttc gaccagcaca acaacatcta caccggcgac
     3481 gaacagctcg acctcttcgt cttcctcggc acgccgaaag aagtcctctc agagtacacg
     3541 gcactgacgg gccgcagccc cgtcc
//
LOCUS       DBNGOH010000218         1976 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2415252, whole genome shotgun sequence.
ACCESSION   DBNGOH010000218 DBNGOH010000000
VERSION     DBNGOH010000218.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1976)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1976)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1976
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2415252"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..785
                     /locus_tag="AC159P_04175"
     CDS             <1..785
                     /locus_tag="AC159P_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5996 family protein"
                     /protein_id="HIR3694681.1"
                     /translation="NHWWNVPLYVSARGLTTSAMPYGRRVFEIEFDFIDHKLDIRASD
                     GARSIALAPRTVAAFYDELMDALRSLGIEVNIYTMPVEVPDPVRFTEDTAHASYDPEY
                     ANRHWRILAQSNKVFNEFRSRFVGKCSPVHFFWGSFDLAVTRFSGRRAPTREGADAIT
                     REAYSHEVISCGFWPGSGNVEAPAFYCYSAPEPNGFAEARVLPGGAFYNKETSGFVLL
                     YEEVRRADSPEDALMDFLQSTYEAGTNLGGWDRAALERTSDG"
     gene            834..1976
                     /locus_tag="AC159P_04180"
                     /pseudo
     CDS             834..1976
                     /locus_tag="AC159P_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009225817.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - metabolic compound salvage
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
BASE COUNT          342 a          722 c          614 g          298 t
ORIGIN      
        1 tcaaccattg gtggaacgtg ccgctctacg tctcggcgcg cgggctgacc acgtccgcga
       61 tgccttacgg gcggcgcgtc ttcgagatcg agttcgactt catcgaccac aagctcgaca
      121 tcagggcgag cgacggcgcg cgctcaatcg ctttggcacc gcgcacggtc gcggcctttt
      181 acgacgagtt gatggacgcg ctgcgctcgc tcggcatcga ggtcaacatc tacacgatgc
      241 ccgtcgaagt gcccgacccc gtccgcttca ccgaagacac cgcgcacgcg tcctacgacc
      301 ccgagtacgc gaaccgccac tggcgcattc tcgcgcagtc gaacaaggtc ttcaacgagt
      361 tccgctcgcg cttcgtgggc aagtgcagcc ccgtccactt tttctggggc agcttcgacc
      421 tcgcggtgac gcgcttttcg ggacgccgcg cgcccacgcg cgaaggcgcc gatgccatca
      481 cgcgcgaagc ctactcgcac gaggtgatta gctgcggctt ctggcccggc agcggcaacg
      541 tcgaggcgcc cgccttctac tgttacagcg cgccggagcc gaacggcttc gcggaggcgc
      601 gcgtgctgcc cggcggcgcg ttctacaata aggaaacgtc cggcttcgtc ctgctctacg
      661 aagaggtgcg gcgcgcggac agccccgaag acgcgctgat ggactttctg caaagcacct
      721 acgaggcggg cacgaacctc ggcggctggg atcgcgcggc gctcgaacga acctccgacg
      781 gctgagggtc gaaaagcccg ccgtcgcttt cccgaaaggt ctaataaaaa ttcatgccaa
      841 cgcttgacgg aaaaactttc gaacgtgtcg cactgctcgt cctcgacagc ctcggcgcgg
      901 gcgagatgcc ggacgccgcc gcgtggggcg acgcgggttc cgacacgctc ggccacatct
      961 gcgcgtcgcg cgcggtcaag ctgccgaact tgcagcggtg gggcttgggc aacatcaggc
     1021 cgctcgcggg cgtgccgcct gtggagagtc cgctcgcgtc cgtggggcgg tgcgcgctgc
     1081 gctcgaacgg gaaggacacg acgacgggcc attgggagat ggtcggcatc gtgctcgaaa
     1141 aggcgttccc gacctacccc gaagggttcc ccgcggaggt cgtcgagcgc ttcgtccgag
     1201 agacgggcgt gcccggcatc ctcggcaact aacccgcctc gggcaccgag atcatcaagc
     1261 agctcggcga ggagcacgtc cgcaccgcga agcccatcgt ctacacctcc gccgactcgg
     1321 tcttccaaat cgccgcgcac gaagaggtca tccccgtccc gcgcctctac gagatttgcg
     1381 agacggcgcg ccgcatcctc cgcggcgagc acgaggtcgg gcgcgtcatc gcgcgcccct
     1441 tcgtcggcgc acccggcgcc tttacgcgca ccgagaaccg acacgactac gccgtcccgc
     1501 ccccgcgcga aaacctgctg ccgcttctct ccgccggcgg cctcgacgtg gtgagcgtcg
     1561 gcaagatcgc ctccatctac gactcgaccg gcgtcacaga agagctgccc ggcaagaaca
     1621 acgaccagtc catcgaccag acgatccacg cgctcaggca aaacacgcgc ggcctcgtct
     1681 tctcgaacct cgtcgatttc gacatgctct acggccaccg ccgcgacgtg gagggctacg
     1741 cccgcgcgct cgaacacttc gacgcgcgcc tgcccgagat cgaggcggcg ctcggcgaca
     1801 cggacctcct catcatcacc gccgaccacg gcaacgaccc gacctaccgc ggcaccgacc
     1861 acacacgcga gtacgtcccg ctcgtcgtct acggcaagcg cgcgcgcgcc ggggtcggcc
     1921 tcggcgaccg cgcctcgctc gccgacatcg gccagaccat cgccgagaac ttcggg
//
LOCUS       DBNGOH010000219         1956 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_32264, whole genome shotgun sequence.
ACCESSION   DBNGOH010000219 DBNGOH010000000
VERSION     DBNGOH010000219.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1956)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1956)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1956
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_32264"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            52..1188
                     /locus_tag="AC159P_04185"
     CDS             52..1188
                     /locus_tag="AC159P_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014098984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HIR3694682.1"
                     /translation="MKTVAFPARPARIFSPGRPPQVELVGELRAVSIAGLEKLLPAHT
                     LMISKMILGANQAAAENLDKYGSADVSFSAPGLSRFRVNIFRQRGTNAIVMRVIPTRP
                     PQWSDYNLPEVLKEVAALKNGLVLVTGPTGSGKSSTLAAVIDLVNESKRYHIITIEDP
                     IEFLHNHKNSTIHQRELHSDTPNFALALRAALRQAPKVILVGEMRDRETIEVALEAAE
                     TGHLVLSTLHTIDASKTVDRIIGVFPKNEEQSIRTRLAQVFRFIVSQRLLPRADGQGR
                     IAAIEILKSTSRTREYMERGEAEGRSLIDAMEQGELEGMQTFDGVIERLVRTGAVARE
                     DALSYATNAGNMQLRLSDVGGVSTVQAPTPKKQDGSGSMLDMLE"
     gene            1499..>1956
                     /locus_tag="AC159P_04190"
     CDS             1499..>1956
                     /locus_tag="AC159P_04190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694683.1"
                     /translation="MSSLFVALLFSLAAQAAAAQDAAGDGFLRLALPPGAEVRVENRR
                     GGVSVEVWGEDAVGVAVSADEAAKKNARAKSPLSIERTEKLLAFVVARGATAAARTRV
                     DLRLRVPANARLKLSTSEGRIEVSGLPASLSAQTVSGDLNVGLPQPLDASV"
BASE COUNT          340 a          690 c          619 g          307 t
ORIGIN      
        1 cgcgccgttt tggtgcggcg cacttcttca tcatatacgt aatccgcctt tttgaaaacc
       61 gtcgccttcc ccgcgcgccc ggcgcgcatc ttctcgccgg gccgcccgcc gcaggtcgag
      121 ctggtcggcg agctgcgcgc ggtctccatc gcgggcctcg agaagctgct gccggcgcac
      181 acgctgatga tctccaagat gatcttgggc gccaaccagg ccgccgccga gaacctcgac
      241 aagtacggct cggcggacgt ctccttcagc gcgcccggcc tctcgcgctt ccgcgtcaac
      301 atcttccgcc agcgcggcac gaacgccatc gtcatgcgcg tcatcccgac gcgcccgccg
      361 cagtggagcg actacaacct gccggaggtc ttgaaggagg tcgccgcgct caagaacggc
      421 ctcgtcctcg tcaccgggcc gacgggcagc ggcaagtcct cgacgctcgc cgccgtcatc
      481 gacctcgtca acgagtcgaa gcgctaccac atcatcacca tcgaagaccc catcgagttc
      541 ctccacaacc acaagaactc gaccatccac cagcgcgagc tgcactccga cacgccgaac
      601 ttcgcgttgg ccctgcgcgc cgcgctcagg caggcgccga aggtcatcct cgtcggcgag
      661 atgcgcgacc gcgagaccat cgaggtcgcg ctcgaagccg ccgagaccgg ccacctcgtc
      721 ctctcgacgc tgcacaccat cgacgcctcg aagaccgtgg atcgcatcat cggcgtcttc
      781 cccaagaacg aggagcagtc gatccgcacg cggctcgcgc aagtcttccg cttcatcgtc
      841 tcgcagcggc tgctgccgcg cgccgacggg cagggccgca tcgccgccat cgaaatcctc
      901 aagtccacct cgcgcacgcg cgagtacatg gagcgcggcg aggccgaagg ccggagcctc
      961 atcgacgcga tggagcaggg cgagctggaa ggtatgcaga ccttcgacgg cgtcatcgaa
     1021 aggctcgtcc gcaccggcgc cgtcgcccgt gaagacgcgc tctcctacgc gaccaacgcg
     1081 ggcaacatgc agctccgcct cagcgacgtg ggcggcgtca gcaccgtgca ggcgccgacg
     1141 ccgaagaagc aggacggctc cggctcgatg ctcgacatgc tcgaataaac gttgcagcag
     1201 gcagcaggca ataggcagca ggcagcaggc agtaaaagcg agaggcttga gagctaacgt
     1261 ctcaagcctc tcgctttgtt actagacgag cgaagatgct ttgaacttta cctctcaagc
     1321 atcttcgctc tgccgtctcg actgcctcct gcctactgcc tgctgcctgc cgcctgctta
     1381 cttgaagtcg aaatttgcgg taatctccgt gcctgtgaag cctcatccaa catccgtgga
     1441 cgggccggga ccaacgtcgg atatgaacac gcggcgggcg cgaaaggcgt cgcggccctt
     1501 gtcgtcgctt ttcgtggcgc tgctcttttc tctcgcggcg caggcggcgg cggcacagga
     1561 cgcggcggga gacggtttcc tgcgcctggc gctgccgccg ggcgcagagg tgcgcgtcga
     1621 gaacaggcgc ggcggcgtga gcgtcgaagt gtggggcgag gacgcggtgg gcgtcgccgt
     1681 ctcggcggac gaggccgcga agaagaacgc gcgcgccaaa tcgcctttga gcatcgagcg
     1741 aacggagaag ctgctcgcct tcgtcgtcgc gcggggcgcg accgccgcgg cgcggacgcg
     1801 tgtcgatctg cgactgcgcg tcccggcgaa cgcgcgcctc aagctctcca cgtccgaagg
     1861 tcggatcgag gtcagcggcc tgcccgcgag tctgagcgcg caaaccgtct cgggcgacct
     1921 gaatgtgggc ctgccgcagc cgctcgacgc gagcgt
//
LOCUS       DBNGOH010000220         2955 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4247814, whole genome shotgun sequence.
ACCESSION   DBNGOH010000220 DBNGOH010000000
VERSION     DBNGOH010000220.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2955)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2955)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2955
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4247814"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1798
                     /locus_tag="AC159P_04195"
     CDS             <1..1798
                     /locus_tag="AC159P_04195"
                     /inference="COORDINATES: protein motif:HMM:NF024743.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HIR3694684.1"
                     /translation="TATEEFHQTYPLAAGGRVSLSNINGRVKITGWDRDEVKVDAVKR
                     ARTQEALRDARIRVEAGAASVHVETEYEGRHWERGDGDRRTYHAARVDYTLTVPRGAR
                     VQDVSVVNGPLDIENLTGPVEASSVNGGVTARGLSGRVDLSVVNGQLEAALDQLGGGN
                     NVSLSAVNGQLNVTLPSDANAHLKAGTVHGSINNDLGLPVREGRYVGRDLEGQLGTGG
                     TRVNLSNVNGTITIRRAADNKPRSEVRNLLSETREAEEFDFDEDKDKDKDKDKDKDGR
                     ELERQIEREARVAERAARAEERAEARAAARAAAQAVRESARESERVAREVEREMSRAF
                     GDGANPDATRQIMRDSATLATKGVPRVRVATFDGPVTVHAWDKPEVRATIVKRAYDDR
                     EMKGIKVSSVVSNDAGPNSEVNIRAEFDKSVAQGALEHDGRIVSWNSGASVELDVYVP
                     RNSVLYVTSGDGRLAVEGVRGEMELHTGDGAIDVTGAAGRLRADTGDGRILIEGFDGE
                     ADAKTGDGRITLDGSFRRLSARTGDGTISLTLPGGVNAVIETDASAVSNDGVAVAEDA
                     NEERRVRRWRVGNGGQVFTLRTGDGRVILQRR"
     gene            2069..>2955
                     /locus_tag="AC159P_04200"
     CDS             2069..>2955
                     /locus_tag="AC159P_04200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694685.1"
                     /translation="MRNFTQIRPLPSLVYAFIILLLTSATASAQEQVASNKAASRKST
                     ASSSRSLPLRRVALKSVVVTGGARVTLTSDAPLDDYSAYRHGERFFVRIPHASAGPGA
                     SLAGGDAFDDVRAEQSGEDLVVSFRVRAGTEARAAQKFNRLEVVFAAQDQQSGGGSNG
                     NAAQSLVKPAATPSPTPAATPTPAPSPTTNTAPPVSAPDGAPAETKAAGASEAVKGTA
                     EPATTKAARYAALLTPEKKNPVKVAKFDKAPVIDGKLDDEVWKSAARFKDFIQNRPGD
                     LIAPSKQTEAYIGYDAKTL"
BASE COUNT          537 a         1018 c         1036 g          364 t
ORIGIN      
        1 gacggcgacc gaggagtttc accagaccta cccgctcgcg gcgggcggtc gcgtgagcct
       61 ctcgaacatc aacggcaggg tcaagatcac gggctgggat cgcgacgagg tgaaggtgga
      121 cgccgtcaag cgcgcgcgca cgcaggaggc gctgcgcgac gcgcgcatca gggtcgaggc
      181 cggcgcggcg agcgtccacg tcgagacaga gtacgaggga cgtcactggg agcgcggcga
      241 cggcgaccgg cggacgtacc acgcggcgcg ggtcgattac acgctgaccg tcccgcgcgg
      301 cgcgcgcgtg caggacgtga gcgtcgtcaa cggcccgctc gacatcgaga acctgaccgg
      361 cccggtggaa gcctcttcgg tcaacggcgg cgtcacggcg cgcggcctct cggggcgcgt
      421 cgatctgtcg gtcgtgaacg ggcagctcga agcggcgctc gaccagctcg gcggcgggaa
      481 caacgtctcg ctgagcgcgg tcaacgggca gttgaacgtc accctcccct cggacgcgaa
      541 cgcgcacctg aaggccggca ccgtgcacgg ctcgatcaac aacgacctcg gcctgcccgt
      601 gcgcgagggg cgctacgtcg ggcgcgacct cgaaggccag ctcggcacgg gcggcacgcg
      661 cgtcaacctc tcgaacgtca acggcaccat caccatccgc cgcgccgcgg acaacaagcc
      721 gcggagcgag gtgcgcaacc tcctctccga gacgcgcgag gccgaagagt tcgacttcga
      781 cgaagataag gacaaagaca aggacaaaga taaggacaaa gacgggcgcg aactcgaacg
      841 acagatcgag cgcgaagcgc gtgttgcgga gcgcgcggcg cgagcagagg aacgcgcaga
      901 ggcacgcgcc gcagcacgcg ccgcggcgca ggcggtgcgc gagagcgcga gggagagcga
      961 gcgcgtcgcg cgcgaagtcg agcgcgagat gagccgagcc ttcggcgacg gggcgaaccc
     1021 ggacgcgacc cggcagatca tgcgcgactc ggcgacgctc gccacgaagg gcgtgccgcg
     1081 cgtacgcgtc gccaccttcg acggccccgt caccgtccac gcgtgggata agccggaggt
     1141 gcgggccacc atcgtcaagc gcgcctacga cgaccgggag atgaagggca tcaaggtcag
     1201 ctccgtcgtc agcaacgacg cggggccgaa cagcgaggtc aacatccgcg ccgagttcga
     1261 caagtccgtg gcgcagggcg cgctcgaaca cgacgggcgc atcgtctctt ggaactcggg
     1321 cgcgtcggtc gagctggacg tgtacgtgcc gcgcaactcc gtgctctacg tcacgtccgg
     1381 cgacgggcgc ctggccgtcg agggcgtgcg cggcgagatg gagctgcaca cgggcgacgg
     1441 cgcgatcgac gtgacgggcg ccgcgggccg cttgcgcgcg gacaccggcg acgggcgcat
     1501 cctcatcgag ggcttcgacg gcgaggccga cgccaaaacc ggcgacgggc gcatcacgct
     1561 cgacggcagc ttccgccgac tctcggcgcg caccggcgac gggacgatct cgctcacgct
     1621 gcccggcggc gtcaacgcgg tcatcgagac ggacgcctcg gcggtctcga acgacggcgt
     1681 ggccgtggcc gaggacgcga acgaggagcg gcgcgtgcgc cgctggcgcg tcggcaacgg
     1741 cgggcaggtc ttcacgctgc gcacgggcga cgggcgcgtg attctccagc gccgctaacg
     1801 cgcggcggcg cgcgtgcgtc ttagaagtcg tccggtgaag agagtggttt tcgaccgtca
     1861 ccggacaaaa cttcgcaggc aaattctttt acattcgatg gcttggcgca ggcgaaacag
     1921 ttgaaacatt agggcgtgag ggcacgtcta acgtgtggcc gcaacaatta gggcgtggtc
     1981 gcttgatcac actctgtata cacgaaaaat ccaaaatatc gtacggaatc cgaatccttg
     2041 cccgagggtt cgccgggagc cgcccgtcat gagaaacttc acgcagattc gccctctgcc
     2101 ctcgttggtt tacgccttca tcatccttct cctcacatcc gcaacggctt cggcacagga
     2161 acaggtcgcc tcgaacaaag cggcgtcgcg caagtctacg gcatcgtcgt cgcgctctct
     2221 gccgctgcgc cgcgtcgcgc tgaagagcgt cgtcgtgacg ggcggcgcgc gcgtgacgct
     2281 cacttcggac gcgccgctcg acgactattc ggcctaccgg cacggcgagc gcttcttcgt
     2341 ccgcatcccg cacgcgagcg cggggccggg cgcctcgctc gcgggcggcg acgcgttcga
     2401 cgacgtgcgc gccgagcaga gcggcgaaga cctcgtcgtc tccttccgcg tccgcgcggg
     2461 cacggaggcg cgcgccgcgc agaagttcaa ccgcctcgaa gtcgtcttcg ccgcgcagga
     2521 tcagcagtcg ggcggcggca gcaacggcaa cgccgcgcag tcgctcgtca agcccgccgc
     2581 gacgccttcg ccgacgcccg cggccacgcc gacgcccgcc ccgagtccga cgacgaacac
     2641 cgcgcccccc gtctcggctc cggacggcgc ccccgccgag acgaaggccg cgggcgcgag
     2701 cgaggccgtc aagggaacgg cagagcctgc gaccaccaag gccgcgcgct acgccgcgct
     2761 gctcacgccc gagaagaaga acccggtcaa ggtcgcgaag ttcgacaagg cgccggtcat
     2821 cgacggcaag ctcgacgacg aggtgtggaa gagcgcggcc cgcttcaagg acttcatcca
     2881 gaaccgcccc ggcgacctca tcgcgccgtc gaagcagacc gaggcctaca tcggctacga
     2941 cgccaagacg ctcta
//
LOCUS       DBNGOH010000221         1629 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1866276, whole genome shotgun sequence.
ACCESSION   DBNGOH010000221 DBNGOH010000000
VERSION     DBNGOH010000221.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1629)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1629)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1629
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1866276"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1202)
                     /locus_tag="AC159P_04205"
     CDS             complement(<1..1202)
                     /locus_tag="AC159P_04205"
                     /inference="COORDINATES: protein motif:HMM:NF017044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HIR3694686.1"
                     /translation="MILRRAKSRVPRVCAAALAVLALFVHAADAQVARNVRVDFKQTT
                     LKNGLRVITVEDHSAPVVALAIAYDVGSRNERAGRTGFAHLFEHMMFKGSENVGAREH
                     NFLIANNGGTMNGTTNEERTLYFEAVPANQLELVLFLEADRMRSLDVSQANLDNQREV
                     VKEERRLRLDNEPYGKSGERQQELMYDNFAYKHSVIGSMEDLGAATVEDVREFFKMYY
                     APNNAVLVLVGDFKTADALARIKKHFEAIPRQPAPPVVEVEEPEQTAERRDTLEDPLA
                     RLARVAVGFKAAPGHTPDYYALQVLSAALQSGQSSRLYQRLVKEKEMVTTVGGFADQS
                     RGASGFYVTATLRPGSKTADVEAAIYEEIERLKREPIADWELKKAKNSTRAGFINSVD
                     TSLSRANLL"
BASE COUNT          263 a          493 c          552 g          321 t
ORIGIN      
        1 agcaggttgg cgcggctgag cgacgtatcg acggagttga tgaagccggc gcgcgtcgag
       61 ttcttcgcct tcttcagctc ccagtcggcg atgggttcgc gcttcaggcg ctcgatctct
      121 tcgtagatgg ccgcctccac gtccgcggtc ttcgagccgg gtctgagcgt cgcggtgacg
      181 tagaagccgg acgccccgcg cgactggtcg gcgaagccgc cgacggtcgt caccatctcc
      241 ttctccttga cgagccgctg gtagagccga gaggactggc cgctctgcaa cgcggcggac
      301 aagacttgca gcgcgtagta gtcgggcgtg tggccgggcg cggccttgaa gccgacggcg
      361 acgcgcgcga gccgcgcgag cgggtcttcg agcgtgtcgc ggcgctcggc ggtctgctcc
      421 ggctcttcga cttccacgac gggcggcgcg ggctggcgcg ggatggcctc gaagtgcttc
      481 ttgatgcgcg cgagcgcgtc cgccgtcttg aagtcgccga cgaggacgag cacggcgttg
      541 ttcggcgcgt agtacatctt gaaaaattcg cgcacgtcct cgaccgtggc ggcgcccagg
      601 tcttccatcg agccgatgac cgagtgcttg taggcgaagt tgtcgtacat cagctcctgc
      661 tgccgctcgc cgctcttgcc gtacggctcg ttgtcgagcc gcagccgccg ctcttctttc
      721 acgacctcgc gctggttgtc gaggttcgcc tggctcacgt ccaggctgcg catgcggtcg
      781 gcttcgagaa agaggacgag ttcgagctgg ttggcgggca cggcctcgaa gtagagcgtg
      841 cgctcctcgt tggtcgtgcc gttcatcgtc ccgccgttgt tggcgatgag gaaattgtgc
      901 tcgcgcgcgc cgacgttctc cgaccccttg aacatcatgt gctcgaagag gtgcgcgaag
      961 ccggtgcggc ccgcgcgctc gttgcgcgac cccacgtcgt aggcgatggc gagcgcgacg
     1021 accggcgcgc tgtggtcttc gaccgtgatg acgcgcaggc cgttcttcag ggtcgtctgc
     1081 ttgaagtcca cgcggacgtt gcgcgcgact tgcgcgtcgg cggcgtggac gaagagggcc
     1141 agcacggcaa gcgccgccgc acagacgcgc ggcacgcgac tcttcgctct acggagaatc
     1201 atgggagctc cttttgtttt gaatcaggca cccgcgggtg ccggggatcg aagacaacaa
     1261 gaatgaggca gattgtaatt acgggaggga gcgcaaaagg ttccactgac tcgacaagat
     1321 tgcggcgcgc gtccagacgc ttggacgtaa tccaaaagaa gggcgaaaag tttaagaggg
     1381 gaagaggcga agagggatga tgaaaagcag cgtgcctctc atcatcgggt tgatgatgag
     1441 aggcagcgtg cctctcgtct tccctcttcg ccttttaacc ttttccccat ttagccttcc
     1501 ggctcagatg tcgaagtaca tgccgaactc cagcgggtgc gggcgcatcc gcaagggctg
     1561 tatctccttc tcccgcttgt aggtgatcca ccggtcgatg agctgcggcg tgaacacgtc
     1621 gcccttgag
//
LOCUS       DBNGOH010000222         1613 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_32637, whole genome shotgun sequence.
ACCESSION   DBNGOH010000222 DBNGOH010000000
VERSION     DBNGOH010000222.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1613)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1613)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1613
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_32637"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..456)
                     /locus_tag="AC159P_04210"
     CDS             complement(<1..456)
                     /locus_tag="AC159P_04210"
                     /inference="COORDINATES: protein motif:HMM:NF024804.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HIR3694687.1"
                     /translation="MSNLRDSAAPEGDPTAAHAFNSPLIAGGTTRSLLKVVRAAQPIS
                     RADLARRLEVNRGTVTEIVKPLIAAGALRENSPEQAAPPRAGRPPIGLTLSARRTFFV
                     GVSLGVLTTRVGAATPDGSLFDEESFDTPHDPAEALARVRAAVERLRAAR"
     gene            804..>1613
                     /locus_tag="AC159P_04215"
     CDS             804..>1613
                     /locus_tag="AC159P_04215"
                     /inference="COORDINATES: protein motif:HMM:NF017984.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding domain-containing
                     protein"
                     /protein_id="HIR3694688.1"
                     /translation="MTTFDVAQRRLHNQRIAATAFENPADVVRWLGAVQAQDYLGSLW
                     ALGLRTRQASEEAVERAVAARSFVRTWPLRGTLHYVAAEDVRWMLSLSGERTIARDAS
                     RYRQLELDEATFAKTNRVLERALRGGQQLTRPELAAALDRAGIRTDEQRLIHLLGRSS
                     LEGLTCYAARRGKQLTFALLDEWAPSAKRLGREEALAELAARYFTSRGPAALQDFVWW
                     SGLTTADARAGLEAAQPRLVREVIDEQTYWLAPSAPPAKVSAPTAHLLPAFD"
BASE COUNT          251 a          538 c          585 g          239 t
ORIGIN      
        1 cctcgccgcg cgcaagcgct cgactgcggc gcgcacccgc gccagcgctt ccgcagggtc
       61 gtgcggcgtg tcgaacgact cttcgtcgaa gaggctcccg tcgggcgtcg ccgcgccgac
      121 ccgcgtcgtc agcacgccga ggctgacgcc gacgaagaag gtgcgccgcg cgctcagcgt
      181 caggccgatg ggcgggcggc ccgcgcgcgg cggtgcggcc tgctcgggcg agttctcgcg
      241 cagcgcgccc gcggcgatga gcggcttgac gatctctgtg acggtgccac ggttgacttc
      301 caagcggcgc gcgaggtccg cgcgcgagat gggctgggcg gcgcggacga ccttcaataa
      361 agagcgcgtc gtcccgcccg cgatgagcgg cgagttgaag gcgtgtgcgg cggtcgggtc
      421 tccttcgggt gcggcggagt cgcgtaggtt agacatgaat tccctccccg gatataaacc
      481 ttgacataaa aaaccggctg acgactcgag aggcgtggca agcgacatgc ctccggcgtg
      541 cccctcgatt gataacacag gaagcgcccg tgtttgtgtg aacatttagc aaactctcgc
      601 cgcgcggcgt tcgcaggtca gggacgcgac cgacattatt gtcgcgccgc ggaaggacgg
      661 cgcgacaagg atgtaatcgc gcggcgcgcg ccacgctccg ctgccgtggc cgaccgcgaa
      721 gattgcctcc ggtgcaaaca ggattcggat ggattagact gccgcgatga gttcggcatc
      781 atgcggcggg cgacaggtga acgatgacga cctttgatgt cgcgcaaagg cgactgcaca
      841 accagcgcat cgcggcgacc gcgttcgaga acccggcgga cgtggtgcgt tggctcggcg
      901 ccgtgcaggc gcaggactac ctcggctcgc tctgggcgct cgggctgcgc acgcggcagg
      961 cgagcgagga ggcggtcgag cgggccgtcg ccgcgaggtc gttcgtccgc acgtggccct
     1021 tgcgcggcac gctccattac gtggccgccg aggatgtccg ctggatgctg tcgctctcgg
     1081 gcgagcgcac catcgcgcgc gacgcgtccc ggtacagaca gctcgaactg gacgaggcga
     1141 ccttcgccaa aaccaaccgc gtgctcgaac gcgccctgcg cggcggccag cagctcacgc
     1201 gcccggagct ggccgccgca ctcgaccgcg cgggcatccg gacggacgag cagcggctca
     1261 tacacttact gggcaggtct tcgctggaag gattgacctg ctacgccgcg aggcgcggca
     1321 agcagttgac gttcgcgctg ctcgacgagt gggcgccgtc ggcgaagagg ttggggcgcg
     1381 aagaggctct ggcggaactc gccgcgcgct acttcacgag ccgcgggccg gcggcgttgc
     1441 aggacttcgt ctggtggtcg gggctgacga cggctgacgc gcgggccggt cttgaggcgg
     1501 cgcagccgcg cctcgtccga gaggtcatcg acgagcagac gtattggctc gccccgtccg
     1561 cgccgcccgc gaaagtctca gcgccgaccg cccacctgct gcccgccttc gac
//
LOCUS       DBNGOH010000223         4522 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2415806, whole genome shotgun sequence.
ACCESSION   DBNGOH010000223 DBNGOH010000000
VERSION     DBNGOH010000223.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4522)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4522)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4522
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2415806"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2180
                     /gene="glnD"
                     /locus_tag="AC159P_04220"
     CDS             <1..2180
                     /gene="glnD"
                     /locus_tag="AC159P_04220"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: protein motif:HMM:TIGR01693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0018177 - protein uridylylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HIR3694689.1"
                     /translation="RERRKRADDFAAALLSERAARYAKFGASVCLQEPNVKESAGGLR
                     DLHTAIWLGHVRTGCATLAELRARGLVSEAEQRTAARAYDFLWRVRHAAHLLTGRKTD
                     RLSLDLQPALAAEFGYESEPHLLDSEKFMRDYYRRARELHQFSESLVARASEYAEREG
                     QLSRWFSRPRAERLAEPFSIGEGRLRLHGDAQLFRQQPLMIFDAFALAQAADVPFGHG
                     LQEAITRSLGAVDRDCRASPEAAEAFLKLLRRRGRVGRALRLMHEVGFLGRYLPEFGR
                     ISLLIQHDLYHHYTVDEHTLKAVEALDELHAGRGKQRAHLRAAFDEVEDPALLYLAIL
                     LHDIGKGRGSGHIPRGAAIAERISRRLQLGAEAAAKVVLLVKHHVTMSHLAQRRDLNE
                     PRVASDFAAEVETLDGLNMLLLLTYADLNGVGPGVWSDWKGTLLWELYQRARTHLTGL
                     DAPDDRVQEISWYKEQVVEKLAGELPVSEVERHLALLPERYTRTTRAEDAAAHLRLIE
                     QLGAEACKTLWRQRAGAGTELTVCARDRHGLFADIAGTLAAHGVEILGAEVNTREDGV
                     AVDVFMLREAATHGAVEEYRRPAIETALAKSVAGESDVAALVERWRTNNAPRRRTALV
                     QARRRRLPRVACDNEASPTATMIEVHAMDEPGLAHKIARALVALRLDIVCAKIATEKS
                     DALDVFYVTDGDGKKLSESALRAAEEALAEQLAPSGAGPRPAH"
     gene            2356..2694
                     /locus_tag="AC159P_04225"
     CDS             2356..2694
                     /locus_tag="AC159P_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018411284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HIR3694690.1"
                     /translation="MKKVEAIIRPHLLDAVKNALQEVNVLGMTVSEVQGYGRQRGHTE
                     TYRGAEYQIEFVPKIKLEVVIPDDIEDFVIHAITKTARTGKFGDGKIFVIPLDDVIRI
                     RTGERGEAAL"
     gene            complement(2744..2914)
                     /locus_tag="AC159P_04230"
     CDS             complement(2744..2914)
                     /locus_tag="AC159P_04230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694691.1"
                     /translation="MDRRGVAWKRFYNINFPARKPENRGDDGLFTAARWPPRASGLLG
                     PVTLTPLGVKRF"
     gene            complement(2877..>4522)
                     /locus_tag="AC159P_04235"
     CDS             complement(2877..>4522)
                     /locus_tag="AC159P_04235"
                     /inference="COORDINATES: protein motif:HMM:NF028442.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HIR3694692.1"
                     /translation="PWRRGAAARGAIVRNQGHGSPANILDLSAASDIPETEGTNVVRM
                     KFVSSAANVIGKPLASAEAATWLNEHFSSTLGDVKQAIDLYFLGGVNHGVYHGTPFSP
                     AGDEWPGRLFYAAVHFGPTNPFWRDFPALNRYIARTQSFLQSGRPDNDVLLYYPVHDL
                     WSVRGKEALVHVGGGHDVGITQGDAQALLDAGYAFDLVSDRQLAGVRGGAGALRTGGH
                     AYQVIVLPESRFMPLKSFEKVFELARAGATVVVRKNLPSDVPGWHDLEARRGALKRLV
                     GQLEFVNAGDGVRSAKVGGGQFLLGDDLKRLLAHAEVRRESMVDQGLHFARRSYPGGS
                     YYFVLNRGDKPLDGWVPLQTGARAVAIFDPMREQAGLAALRAMAGVGAEVFLQLAPGE
                     SVVLKTFDSVVRGPSYGYLENGGATQPLRGTWSVRFDEGGPELPPRVERAELGSWTEY
                     GGDAVKKFSGAATYSLDFGRPAGGGRRLAARLGGRGRERARQTQRGGAGHADHAALQG
                     QSSGFSAEGQEHPGGLRVEPDGQPHRRHGSARRRVEAVL"
BASE COUNT          740 a         1644 c         1508 g          630 t
ORIGIN      
        1 aacgcgagcg gcgcaaacgc gcggacgact tcgccgcggc gctcctttcc gaacgcgccg
       61 cgcgctacgc gaagttcggc gcgtccgtct gcttgcagga gccgaacgtc aaggagagcg
      121 cgggcggcct gcgcgacctg cacacggcca tctggctcgg ccacgtcagg acgggctgcg
      181 cgacgctcgc ggagctgcgc gcgcgcggcc tcgtctccga agcggagcag cgcacggcgg
      241 cgcgcgctta cgatttcctc tggcgcgtcc gccacgcggc gcaccttttg acgggccgca
      301 agaccgaccg cctctcgctc gacctgcaac ccgcgctcgc cgccgagttc ggctacgagt
      361 ccgagccgca cctgctcgac tccgagaagt tcatgcgcga ctactaccgc cgcgcgcgcg
      421 agctgcacca gttcagcgaa tcgctcgtcg cgcgcgcctc cgagtacgcc gagcgcgagg
      481 ggcaactgtc gcgctggttc agcaggccgc gcgccgaacg gctcgccgag cctttctcca
      541 tcggcgaagg gcggctgcgg ctgcacggcg acgcgcagct cttccggcag caacccctga
      601 tgatcttcga cgccttcgcg ctcgcgcaag ccgcggacgt gcccttcggc cacggcctgc
      661 aagaggccat cacgcgcagc ctcggcgccg tggacagaga ctgccgcgcg tcgcccgagg
      721 ccgccgaggc ttttctcaag ctcctgcgcc ggaggggccg cgtcggtcgc gccctgcggc
      781 tgatgcacga ggtcggcttc ctcggtcgct acctgcccga gttcggtcgc atcagcctgc
      841 tcatccagca cgacctctat catcactaca cggttgacga gcacacgctg aaggccgtcg
      901 aggcgctcga cgagctgcac gccggacgcg gcaagcagcg cgcgcacctg cgcgcggcct
      961 tcgacgaggt ggaagacccc gcgctgctct acctcgccat cctcctgcac gacatcggca
     1021 agggacgcgg cagcggccac atcccgcgcg gcgccgcgat agccgagcgc atctcgcgcc
     1081 gcctccaact cggcgccgag gccgcggcga aggtcgtgct cctcgtcaag caccacgtca
     1141 cgatgtcgca cctcgcgcag cgccgcgacc tgaacgagcc gcgcgtcgcc tcggacttcg
     1201 ccgccgaggt cgaaacgctc gacggcctga acatgctgct gcttttgacc tacgccgacc
     1261 tcaacggcgt cggccccggc gtgtggagcg actggaaggg cacgctgctc tgggaactct
     1321 accagcgcgc gcgcacgcac ctcaccggcc tcgacgcgcc cgacgaccgc gtgcaggaaa
     1381 tttcgtggta caaggagcag gtcgtcgaaa agctcgcggg cgagctgccc gtcagtgagg
     1441 tcgagcgcca cctcgcgctc ctgcccgaac gctacacgcg caccacgcgc gcggaggacg
     1501 cggcggcgca cctgcgtttg atagaacaac tcggcgccga agcctgcaag acgctctggc
     1561 ggcaacgcgc cggcgccggg actgaattga ccgtctgcgc gcgcgaccgc cacgggctgt
     1621 tcgccgacat cgccggcacg ctcgccgcgc acggcgtcga aatcctcggc gccgaggtca
     1681 acacgcgcga agacggcgtc gccgttgacg tgttcatgct gcgcgaggcg gcgacgcacg
     1741 gcgcggtcga agagtaccgc cgcccggcca tcgagacggc gctcgccaaa tcggtcgcgg
     1801 gtgagtcgga cgtggcggcg ctggtcgagc gctggcgcac gaacaacgcg ccgcgccgcc
     1861 gcacggcgct cgtccaggcg cgccgccgcc gcctgccgcg cgtcgcctgc gacaacgagg
     1921 cgtcgcccac ggccaccatg atcgaggtgc acgcgatgga cgagccgggc ctcgcgcaca
     1981 agatcgcgcg cgcgctggtc gcgctccgcc tcgacatcgt ctgcgccaag atcgccaccg
     2041 agaagtcgga cgccctcgac gtgttctacg tcaccgacgg agacggcaag aagctctccg
     2101 agtccgccct gcgcgccgcc gaagaagcgc tcgccgagca actcgccccg tccggcgcgg
     2161 ggccacgacc ggcgcactga gagccgagag cctatgtcaa gactgtaggg gcagggcttg
     2221 tccctgcccg ccgttacggt tcgactcaac gtcgaagaca gcagggcgaa cgggccgcgg
     2281 acttgccctg cccctacagc cgtcacgcac gtcgatagtt tcaatacgaa ctctgaatca
     2341 ttcgaggaga tgtgaatgaa aaaagtcgaa gccatcatca ggccgcactt gctggacgcg
     2401 gtgaagaacg cgcttcagga ggtgaacgtg ctcgggatga ccgtcagcga agtgcagggc
     2461 tacgggcgcc agcgcggcca cacggagacc taccgcggcg ccgaatacca gatcgagttc
     2521 gtccccaaga tcaagctcga agtcgtcatc cccgacgaca tcgaagactt cgtcatccac
     2581 gccatcacca agacggcccg caccggcaag ttcggcgacg gcaaaatctt cgtcatcccc
     2641 ctcgacgacg tgatccgcat ccgcaccggc gagcgcggcg aggcggcgct gtaggaagga
     2701 agccgtccga aagggcgcga gcgcccgcct gcttcggacg gcctcagaac ctcttgactc
     2761 cgagcggcgt gagcgtcacg gggccgagca ggcccgaagc cctcggcggc cagcgcgcgg
     2821 cggtgaatag cccgtcgtcg ccccggttct ccggcttcct cgcggggaag ttgatgttat
     2881 agaaccgctt ccacgcgacg ccgcgccgat ccatgtcggc gatgcggttg gccatcaggt
     2941 tcgacacgga gacctccagg gtgttcctgt ccctcagcag agaacccgga actctgaccc
     3001 tgaagggcgg catgatcagc gtgcccagct cctccccgtt gagtctgacg cgcgcgctct
     3061 cggccacgcc ccccaagtcg agcagccagt cgccgccccc ccccggcggg cctgccgaag
     3121 tcgagcgagt aggtcgccgc gccggagaac ttcttgaccg cgtcgccgcc gtactccgtc
     3181 cacgaaccca gctcggccct ctcgacgcgc ggcggcagtt ccggcccgcc ctcgtcgaag
     3241 cgcacagacc aggtgccccg caggggctgg gtcgcgccgc cgttttcgag gtagccgtac
     3301 gaaggccccc gcacgaccga gtcgaaggtc ttgaggacga ccgactcgcc gggcgcgagc
     3361 tggaggaaga cctccgcccc cacgcccgcc atcgcgcgca gggcggcaag gcccgcctgc
     3421 tcgcgcatgg ggtcgaagat ggcgacggcg cgcgcgcccg tttgcagcgg tacccagccg
     3481 tccaacggct tatcgccccg gttgaggacg aagtagtaac tgccgcccgg atagctgcgg
     3541 cgcgcgaagt gcagcccctg atccaccatc gactcgcgcc tgacctctgc gtgcgcgagc
     3601 agtcgcttca aatcgtcgcc gagcaggaac tggccgcccc cgaccttcgc gctccggacg
     3661 ccgtcgcctg cgttgacgaa ctcgagctgg ccgacgagcc gcttcaacgc gccccgccgc
     3721 gcttcgaggt cgtgccagcc gggcacgtct gacggcagat tcttgcggac gacgacggtc
     3781 gcgccggccc gcgccagctc gaacaccttc tcgaaagact tcagcggcat gaagcggctc
     3841 tcgggcagga cgatgacttg ataggcgtgg ccgccggtgc ggagtgctcc cgcgccgccg
     3901 cggacgcccg cgagctgcct gtccgagacg aggtcgaagg cgtagccggc gtccaacagc
     3961 gcctgcgcgt cgccctgcgt gatccctacg tcgtggccgc cgccgacatg gacgagcgcc
     4021 tccttgccgc gcaccgacca gaggtcgtgg acggggtagt agaggagcac gtcgttgtcg
     4081 ggtcgccccg actggaggaa agactgcgtc cgcgcgatgt aacggttgag cgcggggaag
     4141 tctcgccaga aggggttggt cgggccgaag tggacggcgg cgtagaagag gcgtccgggc
     4201 cactcgtcgc ccgcgggcga gaagggcgtg ccgtgataga cgccgtggtt gacgccgccg
     4261 aggaagtaaa ggtcgatggc ctgcttcacg tcgccgaggg tcgaggagaa gtgctcgttc
     4321 agccacgtgg cggcctccgc cgaggccaaa ggcttcccta tcacgttcgc cgccgaggag
     4381 acgaacttca tccgcaccac gttcgtcccc tcggtctcgg ggatgtcgct ggccgcggag
     4441 aggtcgagga tgttggcggg cgagccgtgc ccttggttcc gcacgatggc gcccctcgcc
     4501 gccgcccccc gcctccaggg ag
//
LOCUS       DBNGOH010000224         3349 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2965997, whole genome shotgun sequence.
ACCESSION   DBNGOH010000224 DBNGOH010000000
VERSION     DBNGOH010000224.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3349)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3349)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3349
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2965997"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..975
                     /locus_tag="AC159P_04240"
     CDS             <1..975
                     /locus_tag="AC159P_04240"
                     /inference="COORDINATES: protein motif:HMM:NF019934.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspG"
                     /protein_id="HIR3694693.1"
                     /translation="PFLPFLLLVLLLAPAGVAAQKKPKKALKDSDARRAIAATEGFAL
                     NSGAVKVKDVSIAGASPVTASAEVTLGFRFEPVDDEKGNAPSILKRKRWRAVEFRAGD
                     RNWERIDLLARTVGADRLEQARTALEELVKEFEERQRASKKNGEAEGAAGAGGENKDD
                     VSKSVDAKGGEPLTRGVLTVKQLTALGSSLIAEVAVDASFRLERDARRRWRVAEVLLG
                     EGAGVDVGAAWQAVNAQKGERAREEMRAIREALEAFRRERGFYVEADSEVVLMDHLSP
                     RFIARVIRLDPWGRPYRYGGTRERYVLSSDGADGQRDTADDVTLAAGG"
     gene            complement(1013..1441)
                     /locus_tag="AC159P_04245"
     CDS             complement(1013..1441)
                     /locus_tag="AC159P_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020470378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694694.1"
                     /translation="MFLDIAIGPPTPVTSQPEELLLFLPLGYLFTVLVEVPVLVVGLS
                     RALGLRERFFAGFWLTACTYPIVVLVLPMVFASSPRSHYLLAAETFAPLAECVLFWLA
                     FGKRLDAGAKLRSFVAIVVANVLSFLLGEVLGAFGWFGLV"
     gene            complement(1569..2609)
                     /locus_tag="AC159P_04250"
     CDS             complement(1569..2609)
                     /locus_tag="AC159P_04250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694695.1"
                     /translation="MTANVNLVGGGRGDGTRSSSLAPSASSFGAALLAFCLLAALLVS
                     WQPLWLSVATVFVFAGPHNWMEFRYFLARMPARWGRSKTFFTVGLGGVLLFSVAYALL
                     YWLGQSWYLDAAAWTAVISLWNTGLLVWVASLVWLRGRQRAARRDWSWAFAVGFALAG
                     VAWAAPVWFSLALVYLHPLVALWFLDRQLGRTRPEWRKTYRACLAVLPLVLLAMWARL
                     ASAPDIAETDALAWRITQHAGAGILTGVSSYLLVATHVFLETIHYGVWLVLIPLAGLR
                     AGVFKTDRIPLAVHREGWPRAVRAALALGLFAVLALWLCFWADYATTRDVYFTLAVAH
                     VLAEVPFLIRLL"
     gene            complement(2625..3242)
                     /locus_tag="AC159P_04255"
     CDS             complement(2625..3242)
                     /locus_tag="AC159P_04255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694696.1"
                     /translation="MRSCRKLFAVVALVAAASAPALANIAPAQSGTSGQSKPSKSIAA
                     RMQLRPDAKAKEAKLLIPRAVFEQMRAEIEGRGDSQAAGVATANRFDLTGTQTAFAGL
                     CLSLSLVFGGMWFVNSRAAGRKTARAAAALAVVAFVGAAAGAVAYANAGPPAFARSLN
                     SKILVPEAASYGGPYGEVKVEIVDGDANDYIELRLPKWKGDKEGE"
BASE COUNT          634 a         1124 c         1168 g          423 t
ORIGIN      
        1 ccctttctcc ccttcttgct tttagtcttg ttgctcgcgc ccgcgggcgt ggccgcgcag
       61 aagaagccga agaaggcgct gaaggacagt gacgcgcggc gcgcgattgc ggcgacagag
      121 gggttcgcgc tgaacagcgg cgccgtcaag gtgaaggatg tttccatcgc gggcgcgtcg
      181 ccggtgacgg cgtcggcgga ggtcacgttg ggcttccgct tcgagccggt cgatgacgag
      241 aagggcaacg cgccttcgat cctcaagcgg aagcgttggc gcgcggtcga gttccgcgcg
      301 ggcgaccgca actgggagcg gatcgatcta ctcgcacgaa ccgtcggcgc cgacaggctc
      361 gaacaggcgc gcaccgcgct cgaagagttg gtgaaggagt tcgaggagcg gcagcgcgcg
      421 agcaagaaga acggcgaagc cgagggcgcc gcgggcgcgg gcggcgaaaa caaagacgac
      481 gtgagcaaga gtgtagacgc gaagggcggc gagccgttga cgagaggcgt gctgaccgtc
      541 aagcagttga cggcgctcgg ctcgtctctg atcgccgagg tcgcggtcga tgcttcgttc
      601 cgcctcgaac gcgacgcgcg ccgcaggtgg cgcgtcgcgg aggtcttgtt gggcgaaggg
      661 gcgggcgtcg atgtgggcgc ggcgtggcag gccgtcaacg cgcagaaggg cgagcgggcg
      721 cgcgaagaga tgcgggcgat cagggaggcg ctcgaagcct tccggcgcga gcgcggcttc
      781 tacgtcgaag ccgacagcga ggtggtgttg atggatcacc tcagcccgcg cttcatcgcg
      841 cgcgtcatcc gcctcgaccc gtggggccgc ccctaccgct acggcggcac gcgcgagcgc
      901 tacgtcttaa gctccgacgg cgccgacggc cagcgcgaca cggcggacga cgtgacgctc
      961 gccgccgggg gctgaagagc gaagcccgca cgcggcgcgc ccctcccggc gctcacacca
     1021 gaccgaacca gccgaacgcg cccaacacct ccccgagcaa aaacgacagg acgttcgcca
     1081 cgacgattgc cacgaagctc cgcagcttcg cgcccgcgtc caaacgcttg ccgaaggcga
     1141 gccaaaacag cacgcactcc gcgagcggcg cgaacgtctc cgcggcgagc aggtagtgcg
     1201 agcgcggcga cgaggcgaag accatcggca gcacgagcac cactatcggg tacgtgcacg
     1261 ccgtcagcca gaagcccgcg aagaatctct cgcgcagccc gagcgcccgc gacagcccga
     1321 cgaccagaac cggtacctcg accagcaccg tgaacaggta gccgagcggc aggaagagca
     1381 acaactcctc cggctgcgaa gtgacgggcg tcggcgggcc gatagctatg tcgagaaaca
     1441 taaacgtgtc ggtacggaag gggaaaagac aggaaggggg aaaaggtcaa aaggggaaaa
     1501 ggggaagagg gaaaagacaa tcctcttcct tttcgtcctt tcccctttta cccttttccc
     1561 ctgccgttct aaagcagtcg gatcaggaac ggaacttcgg ccagcacgtg cgcgacggcg
     1621 agcgtgaagt acacgtcgcg ggtcgtggcg tagtcggccc agaagcagag ccacagcgca
     1681 agcaccgcga agaggccgag ggcgagcgcc gccctgacgg cgcgcggcca gccttcgcgg
     1741 tggacggcga gcgggattct gtccgttttg aacacgcccg cgcgcaggcc cgcgagcggg
     1801 atcagcacga gccagacgcc gtagtgaatc gtttcgagaa agacgtgcgt cgcgacgagc
     1861 aggtagctcg aaacgccggt caggattccg gcgcccgcgt gctgcgtgat gcgccacgcg
     1921 agcgcgtccg tctccgcgat gtccggcgcc gacgcgagcc gcgcccacat cgcgagcaat
     1981 accagcggca gcacggcgag gcacgcgcga taggtcttgc gccactcggg gcgcgtgcgt
     2041 ccgagctgcc ggtcgaggaa ccagagcgcg acgagcgggt gcaggtagac gagcgcgagg
     2101 ctgaaccaga ccggcgccgc ccacgctacg cccgcgagcg cgaagccgac cgcgaacgcc
     2161 caagaccagt cgcgccgcgc cgcgcgctgc cgcccgcgca accacacgag cgaagccacc
     2221 cataccagca gccccgtgtt ccacagagag atgaccgccg tccacgccgc cgcgtccagg
     2281 taccagctct gccccagcca gtagagcagc gcgtaagcga cggagaagag aaggacgccg
     2341 cccagcccca ccgtgaaaaa tgtcttcgac cggccccagc gcgcgggcat gcgcgcgagg
     2401 aagtagcgga actccatcca gttgtgcggc cccgcgaaga cgaagacggt ggcgaccgaa
     2461 agccagagcg gctgccagct cacgagcagc gccgcgagca ggcagaacgc gagcagcgcc
     2521 gccccgaagg aagacgcgga aggcgcgagc gacgacgaac gcgtgccgtc gccgcgcccc
     2581 cctccgacga gattcacgtt agctgtcatg ctctgcacgg cgcgctactc cccttccttg
     2641 tcgcccttcc atttcggcag gcgcagctcg atgtagtcgt tcgcgtcgcc gtccacgatc
     2701 tcgaccttca cctcgccgta ggggccgccg taagaggccg cttcggggac gagaatcttc
     2761 gagttcagcg agcgcgcgaa ggcaggcggc ccggcgttgg cgtaagcgac cgcgcccgcc
     2821 gccgcgccga cgaaggcgac gacggccaac gccgccgccg cgcgcgccgt cttcctgccc
     2881 gccgcgcgcg agttgacgaa ccacatcccg ccgaagacga gcgacaaaga caggcacagg
     2941 cccgcgaacg cggtctgcgt gcccgtcagg tcgaagcgat tcgctgtggc cacaccggcg
     3001 gcctgcgaat cgccgcggcc ctcgatctcc gcgcgcatct gttcgaacac ggcgcggggg
     3061 atgagcaact tcgcctcctt cgccttcgcg tcgggcctca gttgcatccg cgccgcgatt
     3121 gacttcgacg gcttggactg gccgctggtg cccgactggg cgggggcgat gttggcgagt
     3181 gcgggcgcgg acgccgccgc gacgagcgcg acgaccgcga agagttttcg gcagcttctc
     3241 atcatttttt ggtgacctcc acgggtggaa gaacgcgcgc attatgcgcc gcttgcaggg
     3301 cggcgctcaa cggaaattcg cggaggcgag ggcggacagt ccgccgccc
//
LOCUS       DBNGOH010000225         6281 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_32871, whole genome shotgun sequence.
ACCESSION   DBNGOH010000225 DBNGOH010000000
VERSION     DBNGOH010000225.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6281)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6281)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6281
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_32871"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            231..1574
                     /locus_tag="AC159P_04260"
     CDS             231..1574
                     /locus_tag="AC159P_04260"
                     /inference="COORDINATES: protein motif:HMM:NF016354.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HIR3694697.1"
                     /translation="MYEFAVTPAITQIRRRGVTITSVEPGTLGAELELEAGDRVVKVN
                     GRAVRDYLDFRFQTGGETELTIEVLKRSGEEWELEVEREEGEDFGIQFENIVPRQCAN
                     ECLFCFCKGNPPDARPALSFRDEDVRLSFLYGNYTTLTSITEDEMRRVVEQRLTPQYV
                     SVHATDLKVRAHLLGVDEERADISDKMRRMMDAGIEIHAQVVLCPTINDGEILERTIR
                     DLAAEYPRVKSVAVVPLGLTRYNTDERLTPVTPEWCRRVIREVGALQKELRAKWGTNF
                     AFLGDEMYLRAGQKVPGRAHYGDYPQIEDGVGMVRSFREDFARLLRRLDMNPPRRPAK
                     LDGTILTGKLFAPVLSELVGELNRRAGTRLRVAAVENRYFGEEIVVAGLMTGECVVAA
                     RERIEGDFVIIPKTAHKSDEPVMLDGTTLEELEQQLGLRVRALDFDGFSRLLTGG"
     gene            1868..5518
                     /locus_tag="AC159P_04265"
     CDS             1868..5518
                     /locus_tag="AC159P_04265"
                     /inference="COORDINATES: protein motif:HMM:NF051698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HIR3694698.1"
                     /translation="MKEVKGLSVRALVAVALLCLFSGSTVLAQTSTSEINGTVKDEQD
                     AIVSGATVTLVDNATGREVTTTTSGEGYFVFPNVPAGTYTVSAENAGFQKKVVQGVRL
                     NVGIPATLNIALTAGNVAEVVTVSASDVSTTINTTNAELSTTVQTQQINDLPLNGRNP
                     LDLAGLQAGVSAGASNRTATINGLRGTYSNLTWDGININDNFIRTDSLFGAAAPSVPG
                     VAEFTLTTQNAGPQDGLGVAQVKLVTPRGGNEYHGTLWEYHRNDVFDANSFFNNAAGL
                     PKAKLIRNQFGAGVGGPFRLPRFGEGGPATYGKDKLFFYAYYEGTRERTDAEINRTVL
                     TQAARQGLFTYQATNGTLQTVNLLALTGRQTDPLATSLINLTPLPNDLTGGDTRNFAR
                     FRFNSPSGSDDDLWGFRIDVDANAHHRFDTSFSRDVLVFPNDTFNDIGEPFPGLPGGG
                     QSPTRTRLSTAWNWTPTSTFNNELRGGFFLQKSKFFTDVTHDRGFRVTFPLISNPEQN
                     FLEQGRNVSTFELIDNAGKSWSNHFIHFGGNYRSLLVDPFNAGGTIPLATLGFNDIGT
                     LNPLTTGLFPGGIGTTDFNNATSILAILTGSLTSVAETFNAASSSSGLLRGQISEQKY
                     RYFSVGPYVGDTWRLRPNLTLNLGLRYEFVSVPREQNGLLVLPIGGLESLLTNAQIEA
                     VSGGGRPLYNNDWNNFAPSGSFAWDPFGEGKTSIRAGYGISYVIDNNITTIDNAANRG
                     GFTRSVTLNSVSGTVSGGIPAIPVPTLTLPFTARSIFSTTDPGFAIFTIDPNLRTPYV
                     QQWNAGVQHEIFRDTVAEVRYVGNRGTKLTRGIDINQQRIFAGGFYEDFLRARFNFVN
                     CGGRVNPTAAQCANRQPLQVLPSFGSFALNQSTFVTAVRQGEAARALDFFITNKSFFF
                     ADYGGGAFGSTQLLSTYLPNPNAYVSDYVGNASYSTYNALQAEIRQRLRGGFDFQANY
                     TWSKSYTDFEGSQANFAGLLDLTQGSELEKRRGINDLTHVFKANAGYELPFGPGKRWL
                     SDGFAGKVFGGMKLTGIFIAQSGRPISFLSTRGTLNRTGRSGSNSVNTNLSVSELQDI
                     TGLFFHPTTGRPLMFDPAVVAAVTGDTSFLRDQNPYFSNPQPGTVGSLHLTPVSGPGI
                     WNMDLGFIKRTQVKESLNVEFRMEAFNVFNKTNFFIGSTALNQNINSSTFGQITQTYD
                     PRILQFAIKVNF"
     gene            5777..>6281
                     /locus_tag="AC159P_04270"
     CDS             5777..>6281
                     /locus_tag="AC159P_04270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694699.1"
                     /translation="MKNIPSNIIQARAGLSAVWVLTLTLLTNGTAAGRPPTAEKMKPE
                     ELIAKHLDAIGPADARQQGRSRIVAGTTLMDLRTGGRGHASGSALIASQGERVLLKAE
                     FDSPSFPFERLGFDGKRLSARPYAPGARSILAEFFLSHDAIFSEGLVGGTLSSAWPLL
                     NLTERRAK"
BASE COUNT         1175 a         2198 c         1897 g         1011 t
ORIGIN      
        1 agtaggcagt tgaagaaata aggaagcagt tctctgctct cctctgtcat cgaagggatg
       61 acgcttcacg gagcacgcgc gtgcttcatc ctgtttcgcg ccttgcttcg tcgccccgcc
      121 ggttgaactt agccacgtca tgaaggatac taacgggttg gcttcggagc gggattcgac
      181 actgtaggga cgaacaacca actgcctgct gcccgctgcc tactgttctt atgtacgaat
      241 tcgcggtcac accggccatc acgcagatac ggcggcgcgg cgtcacgatc acgtcggtcg
      301 agccgggcac gctcggggcg gagctggaac tcgaagcggg cgaccgcgtc gtgaaggtca
      361 acgggcgcgc ggtgcgcgac tacctcgact tccgtttcca gacgggcggc gagaccgaac
      421 tcaccatcga agttttgaag cggagcggcg aggagtggga gcttgaggtc gagcgcgaag
      481 agggcgagga cttcggcatc cagttcgaaa acatcgtccc gcgccagtgc gccaacgagt
      541 gcctcttctg cttctgcaag ggcaacccgc cggacgcgcg ccccgcgctc tcgttccgcg
      601 acgaggacgt gcgcctctcc ttcctctacg gcaactacac gacgctcacc tcgatcaccg
      661 aggacgagat gcgccgcgtc gtcgagcagc gcctcacgcc gcagtacgtc tccgtccacg
      721 cgaccgacct caaggtgcgc gcacacctgc tcggcgtgga cgaggagcgc gccgacatct
      781 ccgacaagat gcggcggatg atggacgccg gcatcgagat tcacgcgcag gtcgtcctgt
      841 gcccgaccat caacgacggc gaaatcctgg agcgcaccat ccgcgacctc gccgccgaat
      901 acccgcgcgt caaatcggtc gccgtcgtgc ctttgggcct gacgcgctac aacacggacg
      961 agcggctcac gccggtcacg cccgagtggt gccgccgcgt catccgcgag gtcggggcgc
     1021 tgcaaaaaga gctgcgcgcg aagtggggga cgaacttcgc gttcctcggc gacgagatgt
     1081 acctgcgcgc gggccagaag gtgccgggcc gcgcgcacta cggcgactac ccgcagatcg
     1141 aggacggcgt cggcatggtg cgctcgttcc gcgaagactt cgcgcgcctc ctgcgaaggc
     1201 tcgatatgaa cccgccgcgc cggcccgcca agctcgacgg aacgatcctg acggggaagc
     1261 tcttcgcgcc cgtcctctcg gaactcgtcg gcgagttgaa ccgccgcgcg gggacgcggc
     1321 tgcgcgtcgc cgcggtggag aacagatact tcggcgagga gatcgtcgtc gccggactga
     1381 tgacgggcga gtgcgtcgtc gccgcgcgcg aacgcatcga gggcgacttc gtcatcatcc
     1441 cgaagacggc gcacaagtcg gacgaacccg tcatgctcga cggcacgacg ctcgaagaac
     1501 tggaacagca actcggcctg cgcgtgcgcg cgctcgactt cgacggcttc tcccgcctgc
     1561 tcacgggcgg ctgaaactct cccacccggt tttcgaaaca caaagcggcg cggtcttcct
     1621 cgaccgcagc tcctccggcg ccggcccctc ttgaatctcc gccttccata aacttgaaga
     1681 tttttccgaa aagctgaatt taacaaaccc ctcacgtgaa agtgaggcgc ggataagttg
     1741 atgctgccca agcagttatc gggccatcca aattgaggaa tacattttgc tagatttttt
     1801 atccctgtgt aaacctgcac cggtagagcc tacatacaca gtcaagccag acaatttagg
     1861 agggaaaatg aaagaagtga aaggtctctc agtgagggca cttgtagcgg ttgcgctgct
     1921 gtgcctcttt tcaggatcaa ccgtccttgc gcaaacctcg acctcagaga taaacggcac
     1981 ggtaaaagac gagcaggatg cgatcgtatc cggggccacg gtgacgctcg tcgataacgc
     2041 gaccgggcgc gaagtgacca ccactacgtc cggggaaggc tacttcgttt tccctaatgt
     2101 tccggccggc acctacacgg tcagcgcgga aaacgccggg ttccagaaga aggtggttca
     2161 gggcgtgagg ctcaacgtcg gcatccccgc cacactgaat atcgcgctga ccgcgggcaa
     2221 cgtcgcggag gtcgtgaccg tttcggcctc ggacgtctcg acgacgatca atacgacgaa
     2281 cgccgagctt tcgaccaccg tccagacgca gcagatcaac gacctgccgc tcaacggcag
     2341 gaacccgctc gacctcgccg gcctccaggc gggcgtctcc gccggggcct cgaaccgcac
     2401 ggccaccatc aacgggctgc gcggcacgta ctcgaacctg acgtgggacg gcatcaacat
     2461 caacgacaac ttcatccgca ccgacagcct cttcggcgcc gccgcgccga gcgtgcccgg
     2521 cgtcgccgag ttcaccctca cgacgcagaa cgcggggccg caggacggcc tcggcgtggc
     2581 gcaggtcaag ctcgtcacgc cgcgcggcgg taacgagtac cacgggacgc tgtgggagta
     2641 tcaccgcaac gacgtcttcg acgccaactc gttcttcaac aacgccgccg gcctgcccaa
     2701 ggcgaagctg atccgcaacc agttcggcgc cggcgtcggc ggcccgttcc gcctgccgcg
     2761 cttcggcgag ggcggccccg ccacctacgg caaggacaag ctcttcttct acgcctacta
     2821 cgagggcacc cgcgagcgca cggacgccga gatcaaccgc acggtgctga cgcaggcggc
     2881 gcggcagggg ctgttcacct accaggcgac taacggcacg ttgcagacgg tgaacctcct
     2941 ggccctgacc ggacgccaga cggacccgct ggccacctcg ctcatcaacc tgacgcccct
     3001 gcccaacgac ctgacgggcg gcgacacgcg taacttcgcg cgcttcaggt tcaacagccc
     3061 gtcgggtagc gacgacgacc tctggggctt ccgcatcgac gtcgatgcca acgcccacca
     3121 ccgcttcgac acctccttca gccgcgacgt gctggtcttc ccgaacgaca cgttcaacga
     3181 catcggcgag cctttccccg gcctgccggg cggtggccag agtccgacgc ggacgcgcct
     3241 gtccacggcc tggaactgga ccccgacctc gaccttcaac aacgagctgc gcggcggctt
     3301 cttcctccag aaatcgaagt tcttcaccga cgtcacgcac gaccggggct tccgcgtcac
     3361 gttcccgctc atcagcaacc cggagcagaa cttcctcgaa cagggccgca acgtgagcac
     3421 cttcgagctg atcgacaacg ccgggaagtc ctggagcaac cacttcatcc acttcggcgg
     3481 gaactaccgc agcctcctcg tcgacccgtt caacgccggc ggcaccatcc cgctcgcgac
     3541 gctcggcttc aacgacatcg ggacgctcaa cccgctcacg acgggcctct tccccggcgg
     3601 gatcggcacc accgacttca acaacgcgac gagcattctg gccattctca cggggtcgct
     3661 cacgagcgtc gccgaaacct tcaacgcggc cagctccagc tccggcctgc tccgcggcca
     3721 gatcagcgag caaaaatacc gctacttcag cgtcgggcct tacgtcggcg acacctggcg
     3781 cctgcgcccc aacctgacct tgaatctcgg cctgcggtac gagttcgtct cggtgccgag
     3841 agagcagaac ggcctgctcg tgctgccgat cggcggtctc gaatcgctgc tcaccaacgc
     3901 gcagatcgag gccgtctcgg gcggcgggcg cccgctctac aacaacgact ggaacaactt
     3961 cgcgccgagc ggcagcttcg cgtgggaccc gttcggcgag ggcaagacct cgatccgcgc
     4021 cggctacggc atctcctacg ttattgacaa caacatcacg accatcgaca acgccgccaa
     4081 ccggggcggc ttcacgcggt cggtgacgct caacagcgtg tcgggaacgg tgagcggcgg
     4141 gatcccggcg atcccggtcc ccacgttgac tctgcccttc accgcccgca gcatcttttc
     4201 gacgaccgac cccgggttcg ccatcttcac gattgacccg aacctgcgga cgccctacgt
     4261 gcagcagtgg aacgccggcg tccagcacga gatcttccgc gacacggtgg ccgaggtgcg
     4321 ctacgtcggc aaccgcggca cgaagctcac gcgcggcatc gacatcaacc agcagcgcat
     4381 cttcgccggc gggttctacg aggacttcct gcgcgcccgt ttcaacttcg tcaactgcgg
     4441 cgggcgggtc aacccgacgg cggcccagtg cgcgaaccgg cagccgctcc aggtgctgcc
     4501 gtcgttcggc tctttcgccc tgaaccagtc aaccttcgtc accgcggtgc ggcagggcga
     4561 ggcggccagg gcgctggact tcttcatcac gaacaagtcc ttcttcttcg ccgactacgg
     4621 cggcggcgcc ttcggctcga cgcagttgct cagcacctac ctgccgaacc cgaacgccta
     4681 cgtctccgac tacgtcggca acgcgtcgta ttccacctac aacgcgctcc aggccgaaat
     4741 ccgccagcgg ctccggggcg gcttcgactt ccaggcgaac tacacctgga gcaagtccta
     4801 caccgacttc gagggtagtc aggcgaactt cgccggcctg ctcgacctga cccaggggag
     4861 cgaactcgag aagcgccgcg gcatcaacga cctgacgcac gtcttcaagg ccaacgccgg
     4921 ctacgagctg cccttcggcc ccggcaagcg ctggctgagc gacggcttcg ccggcaaggt
     4981 cttcggcggg atgaagctga cgggcatctt catcgcccag tcgggccgcc cgatctcgtt
     5041 cctctccacg cgcgggacgc tgaaccgcac ggggcgctcc ggcagcaact cggtgaacac
     5101 gaatctgtcg gtcagcgaac tccaggacat caccggtctg ttcttccacc cgacgacggg
     5161 ccgcccgctg atgttcgacc cggcggtggt cgcggcggtt accggtgaca cctcgttcct
     5221 gagggaccag aacccgtact tctccaaccc gcagcccggc acggtcggca gcctgcacct
     5281 gacgcccgtc agcggccccg gcatctggaa catggacctc gggttcatca agcggacgca
     5341 ggtcaaggag tcgctcaacg tggagttccg gatggaggcg ttcaacgtct tcaacaagac
     5401 caacttcttc atcggcagca ccgcgctgaa ccagaacatc aacagctcga ccttcgggca
     5461 gatcacacag acctacgacc cgcgcatcct ccagttcgcc atcaaggtga acttctaacg
     5521 cggcggggcg ggcgccgcta gcgtgcccgc ccggcgccga atatttcgcg gcgctggtcg
     5581 tccggacggc cagcgccgcc tttttgtacc gggtttgaac tccggcgggc ggcgtggtat
     5641 agctaacgcc gcacgcctgc cccggcgctc ccctccgggg ttcccacacc gatccggcac
     5701 tcattccgcg gctgtgcaag cgcccgtggc cgccgaccaa catctcgaaa gcagccagtg
     5761 gaggattcga ccagagatga agaacatccc ctcgaacatc attcaagcaa gggccgggct
     5821 gagcgcggtc tgggttctta ccctcacgct gttaacgaac ggcacggcgg cgggacgccc
     5881 gccgaccgcg gagaagatga agccggagga gctgatcgcc aagcacctgg atgccatcgg
     5941 gccggctgac gcgcggcagc agggccgcag ccgcatcgtc gccgggacga ccctgatgga
     6001 tttacgcacc ggggggcgcg ggcacgccag cgggtcggcc ctgatcgctt ctcaaggcga
     6061 gagggtgttg ctcaaggccg agttcgactc tccgagcttc ccgttcgagc ggctgggctt
     6121 tgacggcaag cggctctcgg cgagacccta cgcccccggc gcccgctcga tcctggcgga
     6181 attcttcctc agtcatgacg cgatcttcag cgaagggctg gtcggcggca cgctgtcctc
     6241 ggcctggccc ctgttgaact tgaccgagcg cagagcgaag c
//
LOCUS       DBNGOH010000226         1921 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1683241, whole genome shotgun sequence.
ACCESSION   DBNGOH010000226 DBNGOH010000000
VERSION     DBNGOH010000226.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1921)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1921)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1921
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1683241"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..891
                     /locus_tag="AC159P_04275"
     CDS             <1..891
                     /locus_tag="AC159P_04275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694700.1"
                     /translation="WFMLRADGDEAPWIPAGLAAGFVILVAAAAREVVMRRAWTRYTR
                     ELESEMRHGTVARAAIKPAAGARANAQSSVAAMRALQQRIAEMDGANVSAETHREAYR
                     LCEQYLSNFDEVMRTAGSAADIRAALRAGQERVRALQRKHLLAWARGETTRLTQEAQR
                     RVRLSDRIETAQQALEVLDEALRAYPGEPELLQSSVAVRNFIASVKVGQWVEMAERSA
                     FRGKYARAIARYRDALFYLSRADMGEEARDEAATRIHREIELLRARLATTDVVLKTEP
                     TAAPPDPKRVLSVEAREDEF"
     gene            961..1167
                     /locus_tag="AC159P_04280"
     CDS             961..1167
                     /locus_tag="AC159P_04280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694701.1"
                     /translation="MIAQRCPKCRSTRVRRGYEDTLLLLRLIGIYSLLCDNCNLLFKG
                     FALPGTVPPRGSHRRKQRAAKGRR"
     gene            1329..>1921
                     /locus_tag="AC159P_04285"
     CDS             1329..>1921
                     /locus_tag="AC159P_04285"
                     /inference="COORDINATES: protein motif:HMM:NF014606.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HIR3694702.1"
                     /translation="MKAKSLLFTFAVCLLAVPAFAQQQGGGQPAPPSVTIMTGGNSSS
                     NGSSFDGMGVKKYLLGPGDRLALRVFNEPQFNGTLVVDDEGKIFVPFVDEPIIGLCRT
                     DREIRKEIVEKLSKYLKRPQVSLHVAEMRSRPAAVVFGAVRNPTPVDMRRRARLLEVL
                     SVAGSVTEQASGDIQIFHTAELMCPEPEDLVEMANQK"
BASE COUNT          312 a          657 c          685 g          267 t
ORIGIN      
        1 tggttcatgc tgcgcgccga cggcgacgag gcgccgtgga taccggcggg actggcggcg
       61 ggcttcgtca tcctcgtcgc cgccgccgcg cgcgaggtcg tcatgcgccg cgcgtggacg
      121 cgctacacgc gcgagctgga atcggagatg cgacacggga cggtcgcgcg cgccgcgatc
      181 aagccggcgg cgggcgcgcg cgccaacgcg caaagctcgg tcgccgcgat gcgcgcgctc
      241 caacagcgca tcgccgagat ggacggcgcg aacgtctcgg cggagacgca ccgcgaggct
      301 taccgcctgt gcgagcaata cctctcgaac ttcgacgagg tgatgcgcac cgccggttcg
      361 gcggcggaca tccgcgcggc gctgcgggcc gggcaggagc gcgtgcgcgc gttgcagcgc
      421 aagcacctcc tcgcgtgggc gcgcggcgag acgacgcggc tcacgcaaga ggcgcagcgc
      481 cgcgtccgcc tctccgaccg catcgagacg gcgcagcagg cgctggaggt tctggacgag
      541 gcgctgcgcg cctaccccgg cgagccggag ctgctccagt cttcggtcgc ggtgcgcaac
      601 ttcatcgcct cggtgaaggt cgggcagtgg gtcgagatgg ccgagcgctc ggccttccgc
      661 ggcaagtacg cgcgggccat cgcgcgctac cgcgacgcgc tcttctacct ctcgcgcgcc
      721 gacatgggcg aggaggcgcg cgacgaggcg gccacgcgca tccaccgcga gatcgagctg
      781 ctgcgcgcgc ggctcgccac caccgacgtc gtcctcaaga ccgagccgac ggcggcgccg
      841 cccgacccga agcgcgtcct gagcgtcgag gcgagggaag acgagttctg agaggcccgg
      901 cggcggacgc ggaaggctcc gaccgaagac gcggaataac gtgacggcgg gagactctcg
      961 atgatcgccc agcggtgccc gaagtgtcgc agcacgcgcg tgcggcgcgg ctacgaagac
     1021 acgctgcttt tgctgcgcct gattggtatt tacagcctgc tgtgcgacaa ttgcaacctc
     1081 ctgttcaaag ggttcgcgct gcccggcacg gtgccgccgc gcggcagcca caggagaaag
     1141 cagcgcgcgg cgaaaggccg gcgctgaggg cgcacgagcg attcgggcca gaggccgcga
     1201 cggaagatcg ggggacgcgg gcagccgcgg cagccgcttt cgcatctaca cggtcttgcg
     1261 cccgtgccgt cggaagtttt tgtcgcgccg gacttacggg cgcgcgtccc atcccaggaa
     1321 gaatgaacat gaaagccaaa tcgcttctct tcacgttcgc cgtctgcctt ctcgccgtgc
     1381 ccgccttcgc gcagcagcag gggggaggcc agcccgcccc gccctcggtc acgatcatga
     1441 cgggcggcaa ctcctcttcc aacggctcgt cgttcgacgg gatgggcgtc aagaagtacc
     1501 tgctcggccc cggcgaccgt ttggccctgc gcgtcttcaa cgagccgcag ttcaacggca
     1561 cgctcgtcgt ggacgacgag ggcaagattt tcgtgccctt cgttgatgag ccgatcatcg
     1621 ggctgtgccg caccgaccgc gagatcagga aggagatcgt cgagaagctc tcgaagtacc
     1681 tcaaacgccc gcaggtgagc ctgcacgtcg cggagatgag gagccgcccg gcggcggtcg
     1741 tcttcggcgc cgtgcgtaac ccgacgcccg tggacatgcg gcggcgcgcg cgcctgctcg
     1801 aagtcctctc ggtcgcgggc agcgtcacgg agcaggcgag cggcgacatc cagattttcc
     1861 acaccgccga gctgatgtgc cccgagccgg aagacctcgt cgagatggcg aaccagaaga
     1921 g
//
LOCUS       DBNGOH010000227         2862 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3515685, whole genome shotgun sequence.
ACCESSION   DBNGOH010000227 DBNGOH010000000
VERSION     DBNGOH010000227.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2862)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2862)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2862
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3515685"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..558
                     /locus_tag="AC159P_04290"
     CDS             <1..558
                     /locus_tag="AC159P_04290"
                     /inference="COORDINATES: protein motif:HMM:NF041379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYCXC family (seleno)protein"
                     /protein_id="HIR3694703.1"
                     /translation="SMKSKVLWLLLVAAAAGAGCAAKEERRASAGDKFQPAVSHGPAR
                     VAAGPTAQPQQQAADDGHGHGQPGEVPAFETDPASLKSLKPTLSPALFYGKQKEGYEA
                     AKAIPKTLAQLPCYCHCDKGFGHKSLHTCFVDEHAAHCAVCIDEALLAYKLEKEEKLK
                     PEQIRERIIAKYTAEQQSGHEGHGH"
     gene            576..698
                     /locus_tag="AC159P_04295"
     CDS             576..698
                     /locus_tag="AC159P_04295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694704.1"
                     /translation="MKRTLASMLAALVLLFGGASEGRAQARRVKMKIDGYLCGN"
     gene            720..2036
                     /locus_tag="AC159P_04300"
     CDS             720..2036
                     /locus_tag="AC159P_04300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694705.1"
                     /translation="MGRLAFAPKPKDIEITDLKRGLAAFAPRPEKHVSFAALKEALRR
                     AGYTLAAAEITVAGTLRREAEGWSLVADNSAQTFVLDTDALKQKKFEDGARAEVGGGW
                     TTAGTGNDARETIAATSITEPEAARAEDDSPAVSFTRVAFAARTRARRASSAPQPFAG
                     AGSFAPIRTTSPGLTVYGGGAVTPRFTFTRQRLGSLEVNRQSLSLNVSYTPTPRLQLE
                     AEASVSRTSYDDGAGRGAGTGFGNVIVSGKYRFYRAVGEWGDRQAAVRFGLELPTGSK
                     RAPGAARLPRATEFVRQQLSAINGGPAAHADLSYSQTRGRMIFGGNVEGVLRGERAGF
                     RTGHELRANTDLEFVVFPFDYRRPTGEVFAILETSFVRRGAGRVGGREVAGSSATEFY
                     VAPALQYVATPRVVFEASVQLPVARRAGAEVLRTRANVLLGARYLF"
     gene            2080..2580
                     /locus_tag="AC159P_04305"
     CDS             2080..2580
                     /locus_tag="AC159P_04305"
                     /inference="COORDINATES: protein motif:HMM:NF020123.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HIR3694706.1"
                     /translation="MKATLFVLALVAAQSVTLARQSERQSPRSETRSPPVKVFRDTEG
                     RVVRLKGLRGRVVLLNFWATWCPPCRAEIPELVRLQSEHGAALQVVGITHPPERKARV
                     RRLARELKVNYPLVIGTRKTARAFGVGGTLPATVVIDREGKVRELIVGILQPEEFEEK
                     VRPLLR"
BASE COUNT          481 a          907 c         1038 g          436 t
ORIGIN      
        1 tcgatgaaaa gtaaagtgct ttggctcctg ctcgtcgccg cggcggcggg cgcaggctgc
       61 gcggcgaagg aagagcggcg cgcgagcgcg ggcgataagt ttcagccggc ggtgtcgcac
      121 gggcctgcgc gcgtggccgc ggggccgacg gcgcagccgc agcagcaggc cgccgacgac
      181 ggccacggcc acggccagcc gggcgaggtg cccgcgttcg agaccgaccc ggcgagcctg
      241 aagagcctga agccgacgct ctcgcccgcg ctcttctacg gcaagcagaa ggagggctac
      301 gaggcggcga aggccatccc gaagacgctc gcgcagttgc cctgctactg ccactgcgac
      361 aagggcttcg gccacaagag cctgcacacc tgcttcgtgg acgagcacgc cgcgcactgc
      421 gccgtctgca tcgacgaggc gctcctcgcc tacaagctcg aaaaggagga gaagctcaag
      481 cccgagcaga tccgcgagcg catcatcgcc aagtacacgg cggagcagca gtcgggccat
      541 gaagggcacg gccactgaag ccgggaaggg aagcgatgaa gagaacgctc gcctccatgc
      601 tcgcggccct cgtgctgctc ttcggcggcg cgagcgaagg gcgcgcgcag gcgcggcgtg
      661 tgaagatgaa gatcgacggg tatctgtgcg gcaattgagt gtacaacgta cagaaggccg
      721 tgggccgtct cgcattcgca ccgaagccga aggacatcga aatcactgac ctcaagcgcg
      781 gcctcgccgc cttcgcgccg aggccggaga agcacgtctc tttcgccgcg ctcaaggagg
      841 cgctcaggcg cgcgggctac acgctcgccg ccgccgagat aacggtcgcc ggcacgctcc
      901 gccgcgaagc ggagggctgg tcgctcgtcg ccgacaactc cgcgcagacc ttcgtcctcg
      961 acacggacgc cctcaagcag aagaaattcg aagacggcgc ccgcgcggag gtcggcggcg
     1021 gctggacgac tgcgggcacg ggaaacgatg cgcgcgagac gatagccgca acttccatca
     1081 ctgaacccga agccgcacgc gcggaagatg actcgccggc ggtgtcgttc acgcgcgtcg
     1141 cgttcgcggc gcggacgcgc gcgcggcggg cttcgtccgc gccgcagccg ttcgcgggcg
     1201 ccgggtcgtt cgcgccgata cgcacgacga gtccgggcct cacggtctac gggggcggcg
     1261 ccgtcacgcc gcgcttcacg ttcacgcgcc aaaggctcgg gagtctcgaa gtcaaccgcc
     1321 agagcttgag cctcaacgtg tcttacacgc cgacgccgcg tttgcagttg gaggcggagg
     1381 cgagcgtgtc gcgcacctct tacgacgacg gcgcggggcg cggcgcgggc acgggcttcg
     1441 gcaacgtgat cgtgtcgggc aagtatcgct tctaccgcgc ggtcggcgag tggggcgacc
     1501 ggcaggcggc tgtgcgcttc gggttggagt tgccgaccgg gagcaagcgt gcgcccggtg
     1561 cggcgcggct gccgcgggcg accgagttcg tgcgccagca gttgtccgcc atcaacggcg
     1621 ggccggcggc gcacgccgat ctgtcttact cgcagacgcg cgggcgcatg atcttcggcg
     1681 ggaacgtgga gggcgtgctg cgcggggagc gcgcgggctt ccgcacgggc cacgagctgc
     1741 gcgcgaacac ggatcttgag ttcgtcgtct tccccttcga ctaccgccga cccacgggcg
     1801 aagtcttcgc catcctcgaa acgagcttcg tgcggcgcgg cgcgggacgc gtcggcgggc
     1861 gagaggtcgc cgggagcagt gcgacggagt tctacgtcgc gcccgcgctt cagtacgtgg
     1921 ccacgccgcg cgtcgtcttc gaggcgagcg tgcaactgcc ggtcgcgcgc cgcgcgggcg
     1981 ccgaggtgtt gcggacgaga gccaacgtgc tgctcggcgc gaggtatctt ttctgaggtg
     2041 cgttccgaat cgtcagacgt gagcgcgggg cgtgcgttga tgaaggcgac gctgttcgtg
     2101 ctcgcgctcg tcgcggcgca gtcggtcacg ctcgcgcggc agtcggagag acagtcgccg
     2161 cggtcggaaa cgaggtcgcc gccggtcaaa gtgttccgcg acacggaggg gcgcgtggtg
     2221 cgcttgaaag gtttgcgcgg gcgcgtggtg ctgctgaact tctgggcgac gtggtgcccg
     2281 ccgtgccgcg cggagattcc cgaactcgtg aggctccaga gcgagcacgg ggcggcgctg
     2341 caagtcgtcg gcatcacgca cccgcccgag cggaaggcgc gggtgcggcg gctcgcgcgc
     2401 gagttgaagg tcaactaccc tctcgtcatc gggacgcgca agaccgcgcg cgcgttcggc
     2461 gtcggcggca ccttgccggc gaccgtcgtc atcgaccgcg agggcaaggt gcgcgagttg
     2521 atcgtcggca tccttcagcc ggaggagttc gaggagaagg tcaggccgct cctccgttga
     2581 cgcgtttgtg gcataatcgt cccgaccccc ccagtagaaa tgcagaacgc agaatgcgga
     2641 atgatgaatg aaagacagtt ttcggttttc agtaaaggca agaggtctgt cttgaaaccg
     2701 aaaaccgaaa acttattcat cattcataat tccgcattca tcattgttcg ggaaggggta
     2761 ttgagcatga gagggctgct gtatcgagtc gtcgttggcg tgtcgagttt ggcgctgtgc
     2821 gcgtcgttcg tcgcggcgca gactaaagtc aagccgccgt tg
//
LOCUS       DBNGOH010000228         1789 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_33429, whole genome shotgun sequence.
ACCESSION   DBNGOH010000228 DBNGOH010000000
VERSION     DBNGOH010000228.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1789)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1789)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1789
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_33429"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..724)
                     /locus_tag="AC159P_04310"
     CDS             complement(<1..724)
                     /locus_tag="AC159P_04310"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HIR3694707.1"
                     /translation="MSRHLTTTPAADISAFLSERIAAGDFPSAVYLVARGGRAVFHDA
                     LGEAVREPARHAATADTIFDLASLTKPLVTGLLSALEIERGRVALDDKVAKHLSEFDR
                     ADKRDLTVRRLLTHSSGLPAWLPLYLATGGAREKTLAAIAATPLAYEPGARVVYSDLG
                     FITLGLMLQRVAGAELSDLARREIIAPLELARTFFNPDRALQTEVAACESAGNSYERE
                     MCVNEFGERGQAVWREGTIWGEV"
     gene            complement(724..1704)
                     /locus_tag="AC159P_04315"
     CDS             complement(724..1704)
                     /locus_tag="AC159P_04315"
                     /inference="COORDINATES: protein motif:HMM:NF013567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR3694708.1"
                     /translation="MNIRTAVIGVGHLGRQHARIHAALAAEGKSNFVAVCDVNEKTAR
                     EIASEREAEWLTDWRALAGRVDAVSLAVPTESHAEIACALLEAGVHVLVEKPISRTLA
                     EADEMIASAERGGAVLMVGQLERFNPALVALCPHVRAPVYFEIHRVGQFTARSLDIDV
                     VLDLMIHDLDIVQWLVGEGVEVTDVRAVGIPVLTGRVDAANARIEFANGAVANITASR
                     VGTEKIRKMRFFQPHDYVAVDYATRRASISNLAPPSATGSWPGVHVKIPDIEDVEPLR
                     TEIELFLAAARTGGPAPVTGAEGRQALALALRVLERIHEHPITSRLVAEK"
BASE COUNT          281 a          574 c          649 g          285 t
ORIGIN      
        1 gcacctcgcc ccagatcgtg ccctcgcgcc agaccgcttg gccgcgctcg ccgaactcgt
       61 tgacgcacat ctcgcgctcg tacgagttgc ccgcggactc gcaggccgcg acctcggttt
      121 gcagcgcgcg gtcggggttg aagaacgtgc gcgcgagttc gagcggcgcg atgatttcgc
      181 gtcgcgcgag gtcggaaagc tcagcgcccg cgacgcgttg gagcatgaga cccaaagtga
      241 tgaagccgag gtcgctgtag acgacgcgcg cgcccggctc gtaggcgaga ggcgtcgcgg
      301 caatcgccgc gagagtcttc tcgcgcgcgc ctccggtcgc caggtaaagc gggagccacg
      361 ccggaagacc cgagctgtgc gtgagcagtc ggcggacagt aaggtctcgt ttgtctgcgc
      421 ggtcgaactc ggacaagtgc ttcgcgactt tatcgtcgag cgcgacgcga ccgcgttcga
      481 tttcgagtgc ggagaggagt ccggtcacga gcggcttcgt caaggacgcg aggtcgaaga
      541 tggtgtcggc ggtcgcggcg tggcgcgcgg gttcgcgcac cgcttcgccg agcgcgtcgt
      601 ggaagaccgc gcgcccaccg cgcgcgacga ggtagacggc ggaggggaag tcgcccgccg
      661 cgatgcgttc ggagaggaag gcggagatgt ccgccgcggg cgtcgtcgtg aggtgtcggc
      721 tcatcatttc tccgcgacca gtcgtgaagt aatggggtgt tcgtggatgc gttcgaggac
      781 gcggagcgcg agcgcgagcg cctgccggcc ctcggcgccg gtgacgggcg cggggccgcc
      841 cgtgcgcgcg gcggcgagga acaactcgat ctccgtgcgg agcggctcca cgtcttcgat
      901 gtcgggaatc ttgacgtgga cgccgggcca ggagcctgtg gccgaaggcg gcgcgaggtt
      961 gctgatcgag gcgcggcgcg tggcgtaatc gacggcgaca taatcgtgcg gctggaagaa
     1021 gcgcatcttg cgaatcttct cggtgccgac gcgcgaggcc gtgatgttgg cgaccgcccc
     1081 gttggcgaac tcgatgcgcg cgttggcggc gtccacgcgg cccgtcagca cggggatgcc
     1141 gacggcgcgc acgtccgtca cctccacgcc ctctccgacg agccactgca cgatgtcgag
     1201 gtcgtgaatc atcaggtcga gcaccacgtc gatgtcgagc gagcgcgcgg tgaactgccc
     1261 gacgcggtgt atctcgaagt agacgggcgc gcgcacgtgc gggcacagcg cgacgagcgc
     1321 cgggttgaag cgttcgagct gcccgaccat gagcacagcg ccgccgcgct ctgccgacgc
     1381 gatcatctcg tcggcctcgg cgagcgtacg cgagatgggc ttttcgacga gcacgtggac
     1441 gcccgcctca agcagcgcgc aggctatctc cgcgtgcgac tcggtcggca cggcgaggct
     1501 cacggcgtca accctgcccg cgagcgcgcg ccagtccgtc agccactcgg cctcgcgctc
     1561 ggaggcgatc tcgcgcgccg tcttctcgtt cacgtcgcac acggcgacga aattcgactt
     1621 gccctcggcg gccagcgccg cgtggatgcg cgcgtgctgg cgacccagat gcccgacccc
     1681 gatgactgcg gtgcgtatgt tcataagcag taggcagtag gcagtaggcg gtaggcagta
     1741 gaaaagcatc aagcgtttgc tttatgctgc ctaccgtcta ctgcctact
//
LOCUS       DBNGOH010000229         4474 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_33495, whole genome shotgun sequence.
ACCESSION   DBNGOH010000229 DBNGOH010000000
VERSION     DBNGOH010000229.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4474)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4474)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4474
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_33495"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1731
                     /locus_tag="AC159P_04320"
     CDS             <1..1731
                     /locus_tag="AC159P_04320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3694709.1"
                     /translation="VHLDLPAADGAGAGAPTELSTAALFELFARQWRSSVNRDGPVPF
                     ASRESPRAGFTRYEVALRAAAEFVLRKDYRADWETEILEEYTYLSQSQFEAALRARGL
                     RVVTSRPLWNPWIVQHRFEGKFHLSDLGGGPLPLPPTNYLIVGEKVPAGAGVELFERQ
                     SRAISAPNFLSLRGYRHEASGQVMELVERPHQTIDVIPWFESAGQVFVFAKKDFPRPV
                     LNACADQPRLNRANYSGYVTEPISAIVDAGEDDKQQVCRVLRERAGLVAEDVVNVGGR
                     FSYYTSPGGVNELVTSRLVEIRPRRTNFSFPNYTPFTDAGTVRELDAAQVLRACHVGG
                     MFDARLEINIYRLLRELGRSCGPWIGAPIELSVQRQLFAPTDPAQVMPPPAHAAFGRP
                     AALSQSRFLLLSEGSFAERDSDGQTLAEARFEYVRPRRLSTNTLVVLPVARTERGVFV
                     GVEHRDLPAVQSFSGSSRLTAAPAWRLPPTVTHQSDIPPFLKGALKRDFDLRADRAWE
                     LGGAYFPTSGVTPEVVYPFVVEVNLGEGPGGALRFVSLSELIGKPDLLADAHLIIATC
                     RLAHALGALS"
     gene            complement(1897..3426)
                     /locus_tag="AC159P_04325"
     CDS             complement(1897..3426)
                     /locus_tag="AC159P_04325"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009627445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase family protein"
                     /protein_id="HIR3694710.1"
                     /translation="MSTERPQTREELYERIRQGSRDEFILEDMIRLGFWPARGELPHD
                     PAEEIHRRAELLRELGELRTEHRHLYNEEALIKEMRRRRLAESRRKRQETKERRERER
                     RERAEEWRRRQGQEIVYLGEAVSGGLNRTECDAARLQSYGLQPLGTAAEIAAALGVTV
                     GELRFLAFDRKTSTVSHYVRFRIPKKTGGVRQISAPMPRLKAAQHWVLHNILEKIETH
                     DCAHGFRAGRSIVTNARPHVGAEAVVNLDLQDFFPTVSYRRVKGLFRAFGYSAAAATI
                     FGLLCTEPTTEEVVLDGRTYFVATGARHLPQGAPTSPAITNILCRRFDRRLLKAAADL
                     GFTYTRYADDLTFSASGESLRHLRGVLERAKAIVTHEGFTVHPQKTRVLRRSRQQDVT
                     GVVVNSKPNVSRRELKRFRATLYQVEKDGPAGKSWGRSPDVIASLQGFANFVYMVNPV
                     KGAELQQRVRAVAEKYGWEPAAVERRPRSQRPAPTPDKVSHEPEPSQPVPARKKWWKL
                     W"
     gene            complement(3731..>4474)
                     /locus_tag="AC159P_04330"
     CDS             complement(3731..>4474)
                     /locus_tag="AC159P_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008184860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HIR3694711.1"
                     /translation="ARSVYAGADGEVIRVWGRRRIHILERLIPVARKFTVHLLGTGLP
                     SFYVADLGDMSFTLGLSGWTANDWSRAGNFDLLAPRAEVDELTMRVVFDELRRDWRAT
                     PAELASSLHLDRATVLGALGAYTQAGRAIFDLNKRVYRARELSREPLPMERLRFASER
                     EESATRFLSERAVKLDAAESNPEGQLSLRGAVKDKEKTFHPSLNLDGDGRMVRAECTC
                     NWHQQHQLFRGPCEHILALRMEAARRKRL"
BASE COUNT          697 a         1505 c         1499 g          773 t
ORIGIN      
        1 gtccacctcg acctgccggc ggcggacggc gcgggggcgg gcgcgcccac cgagctctcc
       61 accgcggcgc tcttcgagct cttcgcgcgg caatggcgca gcagtgtcaa ccgcgacggg
      121 ccggtcccgt tcgccagccg ggagtcgccg cgcgccggct tcacgcgcta cgaggtcgcc
      181 ctgcgcgcgg ccgccgagtt cgtgctgcgc aaggactacc gcgcggactg ggagacggag
      241 attctggagg agtacaccta cctcagccag tcgcagttcg aggcggcgct gcgcgcccgc
      301 ggcctgcgcg tcgtcacctc ccgcccgctc tggaacccct ggatcgtgca gcaccgcttc
      361 gagggtaaat tccatctctc ggacttgggc ggagggccgc tgccgctacc gccgacgaac
      421 tacctcatcg tcggcgagaa ggtgcccgcc ggcgccgggg tcgaactctt cgagcggcag
      481 agccgtgcga tctcggcgcc gaatttttta agcctcagag gctaccgcca cgaagcgtcg
      541 gggcaagtga tggaactcgt cgaacggccc caccagacca tcgacgtaat tccgtggttc
      601 gaaagcgcgg ggcaggtctt cgtcttcgcg aagaaggatt tcccgcgccc cgtcctcaac
      661 gcctgcgccg accagccgcg cctcaaccgc gcgaactact ccgggtatgt caccgagccg
      721 atcagcgcca tcgtcgatgc gggggaggac gacaagcagc aggtctgccg cgtcctgcgg
      781 gagcgagccg gtctggtcgc ggaggatgtc gtcaacgtcg gcgggcggtt ctcttattac
      841 acctcgcccg gcggcgttaa cgaactcgtc acgtcgcgcc tcgttgaaat ccgcccccgc
      901 cggacgaact tctcgttccc caactacacc cctttcaccg acgccgggac ggtgcgcgag
      961 ttggacgcgg cccaagtcct gcgggcgtgc cacgtcgggg ggatgttcga cgcgcgcctt
     1021 gagataaaca tctaccgcct gctgcgtgaa cttggcagga gctgcggtcc gtggatcggg
     1081 gcgcccatcg agctatccgt ccagcggcag ctgttcgcac caacagaccc cgcgcaggtc
     1141 atgccccccc cggcccacgc cgccttcggt cggcccgcgg ctctatctca gtcacggttc
     1201 ctactcttga gcgagggcag ttttgccgag cgggatagcg acggacaaac gctggccgag
     1261 gcacgattcg agtatgtgag gccacggcgg ctgagcacca acacgctggt cgtcttgcct
     1321 gtggcgagga cggagcgagg ggtcttcgtc ggcgtcgagc accgcgacct gccggcggtt
     1381 caatccttca gcggcagctc aagactgacg gcggccccgg cgtggcgctt gccgccgacg
     1441 gtgacacacc agtcggacat cccgcctttc ctgaaggggg cgttgaagcg cgatttcgac
     1501 ctccgcgccg acagggcgtg ggagctgggt ggagcatact tcccgacctc gggcgtcacg
     1561 cccgaggtgg tttacccgtt cgtcgtcgag gtcaacctgg gtgaaggccc cggcggcgcg
     1621 cttcgattcg tctctttgag cgagttgata gggaagcccg acctgctggc ggacgctcat
     1681 ctcatcatag cgacttgcag actggctcac gccctgggag ctttgtcgta atcgcgtcag
     1741 gcgcgcggag gatgactgcg gaacgcggga taacgaatgc cgtgtgagaa gccgttcgaa
     1801 tgatgacggg caccgtgctt ttcactcatc accctacaag gctcacgccg tgcttttcgc
     1861 tcatcgcctc acaagacgca ccccttggcg ggtgagttac cagagtttcc accacttttt
     1921 ccgggcgggc acaggttggg aaggttccgg ttcgtgcgag actttgtcgg gcgtcggcgc
     1981 cggtctctgc gagcggggac ggcgttcgac cgcggcaggc tcccagccgt acttctcggc
     2041 tacggcgcgg acacgctgtt ggagttcggc gcctttgacg ggattcacca tgtagacgaa
     2101 gttggcgaac ccctggagcg aggcgatcac atccggggag cggccccagc tcttccctgc
     2161 ggggccgtcc ttttcgacct gatagagtgt ggcgcggaac cgcttcaatt ctcgccgcga
     2221 gacgttcggc ttgctgttga cgacgacgcc cgtcacgtcc tgctggcggg agcggcgcag
     2281 caccctcgtc ttctgcgggt ggacggtaaa gccttcgtgc gtgacgatgg ccttcgcccg
     2341 ttcgagcacg ccgcgcaggt ggcgcaggct ctcaccggag gccgagaaag tcaggtcgtc
     2401 ggcgtagcgc gtgtaggtga agccgaggtc ggcggcggcc ttcagcaggc ggcggtcgaa
     2461 gcggcggcag aggatgttgg tgatggccgg gctggtcggc gcgccttgcg gcaggtggcg
     2521 cgcaccggtg gcgacgaaat aggtgcgccc gtcgaggacg acctcctcgg tcgtcggctc
     2581 ggtacagagc agcccgaaga tggtggccgc cgccgccgag tagccgaacg cgcggaagag
     2641 tcctttgacc cgccggtagc tgaccgtggg gaagaagtct tgcaggtcga ggttgacgac
     2701 cgcctccgcc ccgacgtgcg ggcgcgcgtt ggtcacgata gaccgccccg cgcgaaagcc
     2761 gtgcgcgcag tcgtgcgtct cgatcttctc cagaatgttg tgtaagaccc aatgctgcgc
     2821 cgccttcagg cgcggcatcg gcgcggagat ttggcgcacc ccgccggtct tcttgggaat
     2881 ccgaaagcgg acgtagtgcg agacggtgga agtcttgcgg tcgaaggcga ggaagcggag
     2941 ttcgccgacc gtgacgccga gagccgccgc gatttccgcc gccgtgccga gcggctgaag
     3001 gccgtagctc tgcaaacgcg ccgcgtcgca ctccgtacgg ttcagcccac ccgaaaccgc
     3061 ttcgcccaga tagacgatct cttgcccttg cctgcgccgc cactcctccg cgcgctcccg
     3121 ccgctcgcgc tcgcgccgct ccttcgtctc ctgacgcttg cggcgcgact cggcgagccg
     3181 tcgccggcgc atctctttga tgagcgcttc ctcgttgtag aggtgacggt gctcggtgcg
     3241 tagctccccg agttcgcgca gcaactcggc gcggcggtga atctcctccg ccgggtcatg
     3301 cggcagctcg ccgcgggcgg gccagaagcc gagccggatc atgtcttcga ggatgaactc
     3361 atccctcgat ccctgacgga tccgctcgta cagctcttcg cgggtttgcg gtcgttcggt
     3421 tgacatacgg gtgtccgtgc gttcgggaag ggagggttcg attgccacgc gtaacggcca
     3481 gggacttacc cgcgtcccct tcgaaaacgc ctgaggactg ggccgctcaa ctcaagcgtc
     3541 ggggtaatac tgtcgctcat ccgcttcgcg gaagccaaac cggcatagcg acagtattgc
     3601 cccgacgcac agtcgagagc ggaccagtga tgcatgcccc gagctttacg catggacggc
     3661 gatccaccgt ccgggttgca aggcccttgg gcagcccctg gccgttacgc ctggcaatcg
     3721 aaccgagttg tcaaagtcgc ttccggcgcg cggcctccat ccgcagcgcc aggatgtgct
     3781 cgcacggccc acggaagagc tggtgctgct ggtgccagtt gcaggtgcac tcggcgcgca
     3841 ccatgcgtcc gtccccgtcg aggttgagcg acgggtggaa ggtcttctcc ttgtccttca
     3901 cggcgcccct caaggaaagc tgtccttcgg ggttcgactc agccgcgtcg agcttgacgg
     3961 cccgctcgga gaggaagcgc gtcgcgctct cctcgcgctc gctggcgaag cgcagccgct
     4021 ccatcggcag cggttcgcgg ctcaactcgc gcgcgcggta gacccgcttg ttcagatcga
     4081 agatcgcgcg ccccgcctgc gtgtacgcgc cgagcgcgcc gagcaccgtc gcgcggtcaa
     4141 ggtgcaggct tgaggccagc tcggcgggcg tcgcgcgcca gtcccggcgc agctcgtcga
     4201 agacaacccg catggtcagc tcgtccacct cggcgcgcgg ggccagcaga tcgaagttgc
     4261 ccgcgcgcga ccagtcgttc gccgtccagc ccgacagccc caacgtgaaa gacatgtcgc
     4321 ccaggtcggc gacgtagaac gacggcagcc ccgtgcccag caggtgcacc gtgaactttc
     4381 gcgcgacagg tatcagccgc tcgaggatgt gtatgcggcg gcggccccag acgcggatca
     4441 cctctccgtc cgcgcccgcg tagaccgaac gggc
//
LOCUS       DBNGOH010000230         3533 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_766613, whole genome shotgun sequence.
ACCESSION   DBNGOH010000230 DBNGOH010000000
VERSION     DBNGOH010000230.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3533)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3533)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3533
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_766613"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(25..1740)
                     /locus_tag="AC159P_04335"
     CDS             complement(25..1740)
                     /locus_tag="AC159P_04335"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl-D-amino-acid deacylase family protein"
                     /protein_id="HIR3694712.1"
                     /translation="MKKIIIKIVVLLVIGLLLLGMLLPLTSCVAPEQAPEFDVVIRGG
                     TVYDGTGSEPVRADVGLRGDQVVTVGDLSKSGAKMVVDAAGLAVAPGFVNMLSWSTES
                     LIVDGRSQGELRQGVTTQIMGEGSSMGPLSPEMKLRIVSEQGDVKYPIEWTTLAEYLT
                     YLERRGTSQNVASFVGATTIREHVVGLEDKQPTPEQLEEMRRLVRREMEAGALGLGSS
                     LIYAPAFYAKTEELIELCKVAAQYKGKYISHMRSEGGRLTEAVEELIRISREANIPAE
                     IYHLKAAGQDNWSKMDRVVELVEAARREGLKVTADMYTYTAGATGLNASLPPSALSGG
                     YEALFKRIADPVSREQIKQAMMTPTSDWESLYHAAGSPERILLVGFKSEKLKPLTGKS
                     LDAVAKERGTDPFDTILDLMLEDRSRVDTIYFLMSEENIRKQIKLPWVSFGSDEASQA
                     PEGVFLKSNPHPRAYGNFARLLGKYVREEKVITLTEAVRRLSGLPAENLGLDRRGFIK
                     PGMFADVVVFDPATVADRATFEQPHQYATGVRHVFVNGTHVLKDGEHTGAKPGRALWG
                     PGRKQ"
     gene            complement(1737..3056)
                     /gene="purB"
                     /locus_tag="AC159P_04340"
     CDS             complement(1737..3056)
                     /gene="purB"
                     /locus_tag="AC159P_04340"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HIR3694713.1"
                     /translation="MIERYTLPEMGALWSERSKFRKWLDVEIAVCEVHAEMGTIPREA
                     VEEIKSKAAFTVERIGEIEKTTDHDVIAFTTALAENIGEEARFVHYGLTSSDVVDTAN
                     ALLLRDACDLLLKRIDALTAVLKRRAFEFEHTPQVGRTHGVHAEPTSFGLTFALWFDE
                     MRRDRARLERARETVAVGKISGAVGAFAHLDPIVEEKVCERLGLKAAPVSTQVIQRDN
                     YAEYLTTLAIIAASVEKFALQVRHWQRTEVREAEERFKKGQKGSSAMPHKRNPILSER
                     LCGMARLMRGYAVAGLESVALWHERDISHSSAERVVLPDASICLDYMLAKAASLIDNL
                     VVHTDRMLENLNATRGLVYSGQLLLALTRAGVSRETAYEWAQRNAMRAWDEGEDFRAL
                     VIADADIAGRLTREEIERVFSPGTYMRNVGAVFARVFGTGEGEEKPS"
     gene            complement(3131..>3533)
                     /locus_tag="AC159P_04345"
     CDS             complement(3131..>3533)
                     /locus_tag="AC159P_04345"
                     /inference="COORDINATES: protein motif:HMM:NF024609.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4019 domain-containing protein"
                     /protein_id="HIR3694714.1"
                     /translation="LALCLPGCAVDERRSIPSGAQETIDRVTEDIAAGRHDKVYDEAA
                     EEWRDTLGAEESRKILARVRERLGRVNARTLRTGREQQSASAPLSGHALELVYQTTFE
                     RGDAVEQFTLLEREGRWLLARYSVSSDALRP"
BASE COUNT          535 a         1215 c         1146 g          637 t
ORIGIN      
        1 tcagtcctca gtcctcggcc ctcatcactg ctttctgccc ggcccccaca gcgcgcggcc
       61 cggtttggcg ccggtgtgct cgccgtcctt caaaacgtgc gtgccgttga cgaagacgtg
      121 gcggacaccc gtggcgtact ggtgcggctg ctcgaaggtc gcgcggtcgg caaccgtcgc
      181 tgggtcgaag acgaccacgt cggcgaacat gccgggcttg atgaagcccc ggcggtcgag
      241 gccgaggttc tcggcgggca ggcccgacag acggcgcacg gcctccgtga gcgtgatgac
      301 tttctcttcg cggacgtact tgcctaagag gcgcgcgaag ttgccgtagg cgcgcgggtg
      361 cgggttcgac ttgaggaaga cgccttcggg cgcctgcgac gcctcgtccg agccgaagga
      421 gacccacggg agtttgatct gcttgcggat gttctcctcg gacatgagga agtagatggt
      481 gtccacgcgc gagcggtctt cgagcatgag gtcgaggatc gtgtcgaacg ggtctgtgcc
      541 gcgctccttc gccaccgcgt cgagcgactt gccggtgagc ggcttcaact tctccgactt
      601 gaaaccgacg agcaggattc gctcgggcga gccggcggcg tgatacaggc tctcccagtc
      661 gctcgtcggc gtcatcatcg cctgtttgat ctgctcgcga gagacggggt cggcgatgcg
      721 tttgaacaga gcctcgtagc cgccgctcaa agccgaaggc ggcaaggagg cgttgaggcc
      781 ggtcgcgccc gccgtgtagg tgtacatgtc ggcggtgact ttcagcccct cgcggcgcgc
      841 ggcctcgacc agctcgacga cgcgatccat cttcgaccag ttatcttgtc ccgccgcctt
      901 gaggtgataa atctccgcgg ggatgttcgc ctcgcggctg atgcggatca gctcctcgac
      961 ggcctcggtc agtcgcccgc cctcgctccg catgtgcgaa atgtacttgc ccttgtactg
     1021 ggccgcgacc ttgcacagct cgatcagctc ctcggtcttg gcgtagaacg cgggcgcgta
     1081 gatgagcgac gagcctagcc ctagcgcgcc cgcctccatc tcccgccgca cgaggcggcg
     1141 catctcttcg agctgttcgg gcgtgggctg tttgtcttcg aggccgacga cgtgctcgcg
     1201 gatggtggtc gcgccgacga acgacgcgac gttctgcgac gtgccgcgcc gctcaaggta
     1261 ggtcaggtat tcggcgagcg tcgtccactc gatggggtac ttcacgtcgc cctgctccga
     1321 gacgatgcgc agcttcatct cggggctgag cggccccatc gaagacccct cgcccatgat
     1381 ctgcgtcgtc acgccctgcc gcagctcgcc ctgcgagcgc ccgtccacga tgagcgattc
     1441 cgtagaccac gacagcatgt tgacgaagcc gggcgcgacc gcgaggcccg cggcgtcaac
     1501 caccattttc gcgcccgact tcgagaggtc gccgaccgtc acgacctgat cgccgcgcag
     1561 ccccacgtcc gcgcgcacag gctcgctccc cgtgccgtcg tagaccgtgc cgcccctgat
     1621 gacgacatcg aactccggcg cctgctcggg cgcgacgcac gaggtgagcg gcagcagcat
     1681 cccgagcagc agcagcccga tgacgagcag cacgacgatc ttgatgatga tcttcttcat
     1741 gaaggcttct cttctccttc gcccgtcccg aagacgcgcg cgaagaccgc gcccacgttc
     1801 cgcatgtagg tgccgggcga gaaaacgcgc tcgatctctt cgcgcgtgag ccgccccgcg
     1861 atgtccgcgt ccgcgatgac gagcgcgcgg aagtcctctc cctcgtccca cgcgcgcatc
     1921 gcgttgcgct gcgcccactc gtaggccgtc tcgcgcgaga cgcccgcgcg cgtcagcgcc
     1981 agcagcagtt gaccgctgta gaccagtccg cgcgtcgcgt tcaggttctc cagcatgcgg
     2041 tctgtgtgga cgacgaggtt gtcgatgagc gaggcggcct tcgccagcat gtagtcgagg
     2101 cagatcgaag cgtcgggcag cacgacgcgc tcggcggaag agtgcgagat gtcgcgctcg
     2161 tgccagagcg cgaccgactc cagccccgcg accgcgtagc cgcgcatcag ccgcgccatc
     2221 ccgcacagac gctccgacaa gatcgggttg cgcttgtgcg gcatcgccga cgaacccttc
     2281 tgccccttct tgaaccgctc ctcggcctcg cgcacttcgg tgcgctgcca gtggcgcacc
     2341 tgaagcgcga acttctcgac ggacgccgcg atgatggcca gcgtcgtcag gtattccgcg
     2401 tagttgtcgc gctggatgac ctgcgtcgag acgggcgccg ccttcaaccc caggcgctcg
     2461 cacaccttct cttcgactat ggggtcgagg tgcgcgaacg cgccgacggc gccgctgatc
     2521 ttcccgacgg ccaccgtctc gcgcgcgcgt tcgagccgcg cgcggtcgcg ccgcatctcg
     2581 tcgaaccaga gcgcgaacgt caggccgaac gaggtcggct cggcgtggac gccgtgcgtg
     2641 cggcccacct gcggcgtgtg ttcgaactcg aacgcgcggc gcttcaggac tgccgtcagc
     2701 gcgtctattc tttttaagag gaggtcgcac gcgtcgcgca gcaggagcgc gttcgccgtg
     2761 tccaccacgt ccgaagaggt cagcccgtag tggacgaagc gcgcctcctc gccgatgttc
     2821 tcggcgagcg ccgtggtgaa cgcgatcacg tcatggtcgg tcgtcttctc gatctcgcct
     2881 atgcgctcga ccgtgaaggc ggccttactt ttaatctcct cgacggcctc gcgcgggatc
     2941 gtccccatct cggcgtggac ttcgcacacg gcgatctcaa cgtcgagcca cttccgaaac
     3001 ttgctccgct ccgaccagag cgcgcccatc tcgggcaggg tgtagcgttc tatcatctcg
     3061 tgaaccgtga atcgtaaacc gagacaagtg aaaaccggat ttcctcttgt cacggtttac
     3121 ggtttacttg ttacggcctt agtgcgtccg agctgaccga gtagcgggcg agcagccagc
     3181 gcccttcgcg ttcgaggagc gtgaactgct cgacggcgtc gccgcgctcg aaggtggtct
     3241 ggtagacgag ttcgagcgcg tggcccgaga gcggggcgga cgcgctctgc tgctcgcggc
     3301 ccgtgcggag cgtgcgcgcg ttcaccctgc cgagccgctc gcgcacgcgc gcgagtatct
     3361 tccggctctc ctcggccccg agcgtgtcgc gccactcctc agccgcctcg tcgtagacct
     3421 tgtcgtggcg accggcggcg atgtcctcgg tcacgcggtc gatggtctcc tgcgcccccg
     3481 acggaatcga gcgccgctcg tccacggcac agccgggaag gcacaaggca aga
//
LOCUS       DBNGOH010000231         2072 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4799188, whole genome shotgun sequence.
ACCESSION   DBNGOH010000231 DBNGOH010000000
VERSION     DBNGOH010000231.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2072)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2072)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2072
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4799188"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..878)
                     /locus_tag="AC159P_04350"
     CDS             complement(<1..878)
                     /locus_tag="AC159P_04350"
                     /inference="COORDINATES: protein motif:HMM:NF024299.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3694715.1"
                     /translation="MRRQGSVLFLFAVVFALSAVALNGCAPAGEVGKTPAPAASPGGG
                     KIPVTTSSEEARKEFLQGRDLAERLLAQDSIQHFDKAVALDPNFALAELGRANASPTA
                     KEFFDHLKKAVSLAGKASEGERLLILANEAGANGEAARQKEHLDKLVAAHPHDERAHF
                     NLGGYHFGQQDFKQAIEHYRKATELAPNYSPAYNILGYAYRQNGDYANAELAFKKYIE
                     LIPNDPNPYDSYAELLLKMGKYEDSITQYRKALSIDPNFVASRNGIAAALLYLGKPEE
                     AAAELQALNDKARGDG"
     gene            complement(1233..>2072)
                     /locus_tag="AC159P_04355"
     CDS             complement(1233..>2072)
                     /locus_tag="AC159P_04355"
                     /inference="COORDINATES: protein motif:HMM:NF017183.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig domain-containing protein"
                     /protein_id="HIR3694716.1"
                     /translation="NWFKPGWVATFGTLTGTINDDGTAVTGAAPGFSDEAGQDYRLAA
                     ASACVNAGTALNASVLPAHDLLRQYVKHRASEPRPADGLVDIGAFEFGSATPADLVIS
                     TASLPNATTGAAYSATLGAAGGVAPHLWTLTAGSLPPGLTLDAASGVISGTPSAAGSY
                     AFTAQVADAQSPPDTASRALSITVNAPPAPPPPSITTTSLPDARRNKSYSRTLQATGG
                     VAPYTWSLASGALPPGLSLNASTGVISGTPTTRGAWSFTVRVEDTQVPPASDTQALSI
                     SVR"
BASE COUNT          310 a          637 c          763 g          362 t
ORIGIN      
        1 tcgccgtcgc cccgcgcctt gtcgttgagg gcctgtagct cggccgccgc ctcctcgggc
       61 ttacccaggt agagcagggc ggcggcgatg ccgttgcgcg acgcgacgaa gttcgggtcg
      121 atggagagcg ccttgcggta ctgggtgatc gagtcttcgt acttgcccat cttcagcagc
      181 agctcggcgt acgagtcgta cgggttcggg tcgttgggta tcagctcgat gtatttcttg
      241 aacgccagct cggcgttcgc atagtcgccg ttctgccggt aggcatagcc gaggatgttg
      301 taggcgggcg agtagttcgg ggccagctcg gtcgccttcc ggtagtgctc gatggcctgc
      361 ttgaaatcct gctggccgaa gtggtagccg ccgaggttga agtgcgcccg ctcgtcgtgc
      421 gggtgggcgg cgacgagctt gtcgaggtgc tccttctgcc gcgccgcctc gccgttggcc
      481 cccgcttcgt tggcgaggat gaggagcctc tccccctccg aagccttgcc cgcgaggctg
      541 accgctttct tcaggtggtc gaagaattcc ttcgcggtcg gggaagcgtt ggcccggccc
      601 agttccgcca acgcgaaatt cgggtcgagc gcgacggcct tgtcgaaatg ttgaatcgag
      661 tcctgcgcca gcagccgctc ggcgaggtcg cgcccctgca agaactcctt tcgcgcttcc
      721 tccgaagagg tcgtgacggg aatcttcccg ccacccggcg aagcggcggg ggcgggggtc
      781 ttcccgacct cgccggccgg ggcgcaacca ttcagcgcca cggcactcag cgcgaagacg
      841 acggcaaaca ggaaaagcac ggaaccttga cggcgcattg ggagcctcct tcgattgtgt
      901 gtcggactga tgactcgcag ttgcgggggt aacggagtcg ggattcttta acggcgaggc
      961 ggattaagcc gtgccggcta tcaagcggga aggggacgcc gaacggcgtc gcggatcacg
     1021 tgtaataggc cgcacttcga acgcggactg accgtgaacg ggtatgaacg gtgggtgaga
     1081 ctatgcgaaa gacgaggccg cgtcaaggac ttgagcggaa ttttacaata gccgatcaga
     1141 cgaacggaat ttaatccgat cgtttgggta aaacccatcc gcgggcgggc ctgccttagc
     1201 acggctctcc caggcgcgat gcgcgacgcg ccctagcgca cgctgatcga gagcgcctgc
     1261 gtgtcgctcg ccgggggcac ctgagtgtcc tcgacgcgca ccgtgaagct ccaagcgcct
     1321 ctcgtggtcg gggtgccgct gatgacgccg gtcgaggcgt tgagcgagag tccggggggc
     1381 agcgcgccgg aggcgagcga ccaggtgtag ggggcgacgc cgcccgtcgc ctgaagcgtc
     1441 cggctgtaag acttgttgcg ccgcgcgtcg ggcagactcg tcgtggtgat gctgggcggc
     1501 ggcggcgcgg gcggcgcgtt gaccgtgatc gagagcgcgc gcgaagccgt atcgggagga
     1561 ctctgcgcgt ccgcgacctg cgccgtgaag gcgtagctcc cggcggcgga cggcgtgccg
     1621 ctgataacgc cgctcgcggc gtcgagcgtg agacccggcg gcagactgcc cgcggtcaac
     1681 gtccagaggt gcggcgcgac gccgcccgcg gcgcccagcg tcgccgaata ggcggcgccc
     1741 gtcgtcgcgt tggggaggct cgcggtgctg atgacaaggt ctgcgggcgt ggcgctgccg
     1801 aactcgaagg cgccgatgtc tacaagtccg tcggcggggc gcggctcgct cgcacggtgt
     1861 ttgacgtact ggcgtaggag gtcgtgcgcg ggcaggacgc tggcgttgag ggcggtgccc
     1921 gcgttcacgc aggccgacgc ggcggcgagg cggtagtcct gcccggcctc gtccgagaag
     1981 cctggcgcgg cgcccgtcac cgcagtcccg tcgtcgttga tcgtccctgt gagcgtgccg
     2041 aaggtggcga cccatccggg cttgaaccag tt
//
LOCUS       DBNGOH010000232         3936 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2417024, whole genome shotgun sequence.
ACCESSION   DBNGOH010000232 DBNGOH010000000
VERSION     DBNGOH010000232.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3936)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3936)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3936
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2417024"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(83..424)
                     /gene="ytxJ"
                     /locus_tag="AC159P_04360"
     CDS             complement(83..424)
                     /gene="ytxJ"
                     /locus_tag="AC159P_04360"
                     /inference="COORDINATES: protein motif:HMM:TIGR04019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol system redox-active protein YtxJ"
                     /protein_id="HIR3694717.1"
                     /translation="MVSTGNHFQTVADAGALDTLFARSHEAPVLLFKHSNACPISAAA
                     YEQMSRVKGDVSIVVVQQSRDLSREVETRTGVRHESPQVLVLRDGAAVWTASHFGVTA
                     AAVEQALREYE"
     gene            complement(620..3547)
                     /locus_tag="AC159P_04365"
     CDS             complement(620..3547)
                     /locus_tag="AC159P_04365"
                     /inference="COORDINATES: protein motif:HMM:NF024180.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing tetratricopeptide repeat
                     protein"
                     /protein_id="HIR3694718.1"
                     /translation="MPRIGTIIKGLLVCSILVGGAPLAAAPARANAGPPAAQAGDGAL
                     AEREKGRAQLRQGKAAEALIHLESALKLFQQAGDKLGEASVNDLLGELYERQGRYDVA
                     LKNFESAYEYYAGEAARPANNIQVVKALSPKDFAYNANLMLSKIGNVHLRRGDRAKAR
                     AAFARMRVTKPDKQSLEAAAQTSVTSGNPLAGANQVKRGITGIFGGGSKKPSASSPTA
                     AVNSVADTANRLQAPFTAYRETVIYTTYELGLGRVAYLSGQYEQAGKHFDNVLSATVG
                     SLPVVGKLRQTRRYRAAARTGLGDIAFQQGNYAEAVKLYDAAAKSAQSDERLDLMWPA
                     QRGTGRSLWAQSAQERDPAKSAKLRAESLAAFRSALSTIETIRQGSLRADEARTTFLA
                     TTEDVFDEASAAFAESALLAQGSSAADAPLAGPALEHAAEGLRVVEQGRARSLLDMLG
                     ETGAEITEGVPADLLQRKQENQARQQEIASQLTGVTVSADGPKRSLSELEEELNRLQT
                     EYDSIENQIRAASPRYAALTTTQPLTLAEIQQRVLDEQTALLEYSLGEKTSYLYAATK
                     TGAALYRLPARAEVERQAVAVREQIIPATLRRALTDSTGETKRGITVSSEGAGSLAAG
                     NYAGVANALYKMVVEPAARFAGERRLLIVADGALNYVPFHALVTARPASGAEYATLSY
                     VLKTNETVYAPSASVVAAIRQQAQASGGAKTGGSMLLVADPVFGPGDARAKGAPAPAS
                     DTGAQADASRGLSGVDNAVEDINAGRESAGGPPSTTAGGVTLARLAGTREEAQQIAGL
                     ARTTGTKADVWLDLDANESNVEARDLRQYRVVHFATHGLLNAERPQFTGVVLSLVGNK
                     ASEDGFLRTDEIFNLKLSSPLVMLSACETGLGKEKKGEGVIGLTRAFMYAGAPTVGVS
                     LWSVADKSTADLMTDFYRNLFAKEGTAPAAAMRAARQKMIADKKYSAPFYWAPFVLVG
                     DWQ"
     gene            complement(3698..>3936)
                     /locus_tag="AC159P_04370"
     CDS             complement(3698..>3936)
                     /locus_tag="AC159P_04370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694719.1"
                     /translation="CYWKPAPEQWERLKAAAGAGRVAEKRNADLAKSQAPLPAPSLAR
                     GIKVKKDEPAPSVVRMNASAESDVLLTKIKLVHK"
BASE COUNT          531 a         1315 c         1396 g          694 t
ORIGIN      
        1 catcgcttcg ctcataaggg ccgtgacagg tgacgagtga tgagaaaaga cggttttttc
       61 aactcgtcac tcgtcacttc ttttattcgt attcccgtag cgcctgctcg accgcggccg
      121 ccgtgacgcc gaagtgcgag gccgtccaga ccgccgcgcc gtcgcgcagc acgagcacct
      181 gcggcgactc gtgccggacg ccggtgcgcg tctcgacctc gcgcgagagg tcgcggctct
      241 gctgcacgac gacgatcgac acgtcgccct tcacgcgcga catctgctcg taggcggcgg
      301 cgctgatcgg gcacgcgttg ctgtgtttga agaggaggac gggcgcctcg tgcgagcgcg
      361 cgaagagcgt gtcgagcgcg cccgcgtcgg cgaccgtctg aaagtggttt cccgtcgaga
      421 ccatcatcgt cccccgcgtg tacgaaaatt gtttgagcgg ctgcgctgat attagctcac
      481 ccgcgacgca agcaacaatt gcggaatgcg gatttcggat cgcggattga aaggcggaga
      541 agattgatga caaaggcgcc tccataaatc gtcaccgctt tacgaatccg cgatccgaaa
      601 tccgcattcc gcaatcgtct tactgccagt cgccgacgag gacgaacggc gcccagtaga
      661 agggcgcgct gtacttcttg tcggcgatca tcttctgccg ggccgcgcgc atcgccgccg
      721 cgggcgcggt gccttctttg gcgaagaggt tccggtagaa gtccgtcatc aggtcggcgg
      781 tggacttgtc ggccacggac cacaggctca cgccaacggt cggcgccccc gcgtacatga
      841 aggcccgcgt caacccgatc acgccttcgc ccttcttctc tttgccgagg cccgtctcgc
      901 aggccgagag catgacgagc ggggaggaga gtttgaggtt gaagatttcg tcggtgcgca
      961 ggaagccgtc ctcgctcgcc ttgttgccga cgagcgagag gacgacgccg gtgaactgcg
     1021 ggcgctcggc gttcagcagc ccgtgcgtgg cgaagtggac gacgcggtac tggcgcaggt
     1081 cgcgcgcctc gacgttggac tcgttggcgt cgaggtcgag ccacacgtcg gccttcgtgc
     1141 ccgtcgtcct ggcgaggccg gcgatctgct gcgcctcttc gcgcgtgccc gcgaggcgcg
     1201 cgagcgtgac gccgcccgcc gtggtcgagg gcggcccgcc cgcgctctcg cgcccggcgt
     1261 tgatgtcctc gacagcatta tcgacgccgg agagtccgcg cgacgcgtcc gcctgcgcgc
     1321 cggtgtccga cgcaggcgcg ggcgcgcctt tcgcgcgcgc gtcgccgggg ccgaacacgg
     1381 ggtctgcgac gaggagcatc gagccgcccg tcttcgcgcc gccggacgct tgcgcctgct
     1441 ggcggatggc ggcgacgacc gaggccgaag gcgcgtagac cgtctcgttc gtcttgagca
     1501 cgtacgagag cgtcgcgtac tcggcgcccg aagcgggcct cgccgtgacg agcgcgtgga
     1561 acgggacgta attgagcgcg ccgtcggcga cgatgagcag gcgccgctcc cctgcgaacc
     1621 gcgccgccgg ctcgacgacc atcttgtaga gcgcgttcgc gacgcccgcg tagttgcccg
     1681 cggcgagcga gcctgcgccc tcgctgctga cggtgatgcc gcgcttggtc tcgccggtcg
     1741 agtcggtcag cgcgcggcgg agcgtggccg ggatgatctg ctcgcgcacg gccacggcct
     1801 gccgctcgac ctcggcgcgc gctggcaggc ggtagagcgc cgcgcccgtc ttcgtcgccg
     1861 cgtacaggta cgaggtcttc tcgccgaggc tgtattcgag gagcgccgtc tgttcgtcga
     1921 gcacgcgctg ctggatttcg gcgagcgtca ggggctgcgt cgtcgtgagc gcggcgtagc
     1981 gcgggctggc cgcgcgaatc tggttctcga tgctgtcgta ctcggtttgc agccggttca
     2041 actcctcttc gagttcgctg agcgaacgct tcggcccgtc ggcagagacg gtgacgcccg
     2101 tgagctgcga ggcgatctcc tgctgccgcg cctgattctc ctgcttgcgc tggaggaggt
     2161 ctgcgggcac gccttccgta atctccgcgc cggtctcgcc cagcatgtcg agcagcgagc
     2221 gggcgcggcc ctgttcgacg acgcgcaagc cttcggcggc gtgttcgagc gccggccccg
     2281 cgagcggcgc gtccgcggcg gaagaccctt gcgccagcaa cgccgactcg gcgaaggcgg
     2341 cggacgcctc gtcgaaaacg tcttcggtcg tggcgaggaa ggtggtgcgc gcctcgtccg
     2401 cgcgcaggct cccctgccgg atcgtctcga tggtcgagag cgccgagcgg aaggcggcga
     2461 gcgattcggc gcgcagcttc gcggacttcg ccgggtcgcg ctcctgcgcc gattgcgccc
     2521 acaggctgcg tcccgtgccg cgctgcgcgg gccacatcag gtcgaggcgc tcgtccgatt
     2581 gcgcgctctt ggcggcggcg tcgtagagtt tgacggcctc cgcgtagttg ccctgctgga
     2641 aggcgatgtc gccgaggccc gtgcgcgcgg cggcgcggta gcggcgcgtc tgcctgagtt
     2701 tgccgacgac gggcaggctc ccgacggtcg ccgagagcac gttgtcgaag tgcttcccgg
     2761 cctgctcgta ctggccgctc aggtaagcga cgcgaccgag gccgagttcg taggtcgtgt
     2821 agatgaccgt ctcgcggtag gcggtgaagg gcgcctgaag tctgttggcc gtgtcggcga
     2881 cgctgttcac ggcggcggtc ggcgaagagg cgctgggctt cttggagccg ccgccgaaga
     2941 tgccggtgat gccgcgcttg acctgattgg cgcccgcgag cgggttgccg ctcgtcaccg
     3001 aggtttgcgc ggcggcttcg agcgactgct tgtcgggctt cgtcacgcgc atgcgcgcga
     3061 aggcggcgcg cgccttcgcg cggtcgccgc ggcgcaggtg gacgttgccg atcttcgaga
     3121 gcatgaggtt cgcgttgtag gcgaaatcct tcggcgagag cgccttcacg acctggatgt
     3181 tgttggcggg gcgcgcggcc tcgcccgcgt aatactcgta tgccgactcg aagttcttga
     3241 gcgccacgtc gtagcggccc tgccgctcgt agagttcgcc caggaggtcg ttgaccgaag
     3301 cctcgcccag tttgtcgccc gcctgctgga agagcttgag cgcgctctcc agatggatca
     3361 gcgcctcggc ggccttgccc tgccggagct gcgcgcggcc cttctcgcgc tcggccagcg
     3421 cgccgtcgcc cgcctgcgcc gcgggtgggc cggcgttcgc gcgcgcgggc gcggcggcga
     3481 gcggcgcccc gccgacgaga atcgaacaga cgagtagtcc cttgatgatc gttccgatgc
     3541 gtggcattgc ttttaccctt gtgcctttct ggttcgacgc cgacgcgacc gctcaagcaa
     3601 ctgcgcccgg tgcgatgcgt gtcggtcgct aacgatacct gccctaagct cgtaacgcgc
     3661 aaaatcgcgg cggcgcggat cagaacgggg cgccgcttca cttgtggacg agcttgatct
     3721 tcgtcaacag cacgtccgac tccgcggagg cgttcatgcg gacgaccgac ggcgccggct
     3781 cgtccttctt caccttgatg ccgcgcgcga gcgagggcgc gggcagcggc gcctgcgact
     3841 tggcgaggtc ggcgttgcgt ttctccgcga cgcggcccgc gcccgccgcc gccttcaaac
     3901 gctcccactg ttcgggcgcg ggcttccagt aacagt
//
LOCUS       DBNGOH010000233         1614 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2967250, whole genome shotgun sequence.
ACCESSION   DBNGOH010000233 DBNGOH010000000
VERSION     DBNGOH010000233.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1614)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1614)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1614
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2967250"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1481)
                     /locus_tag="AC159P_04375"
     CDS             complement(<1..1481)
                     /locus_tag="AC159P_04375"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF019334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HIR3694720.1"
                     /translation="MLVADTILQGRYRVVRPLGRGGMGAVYEATDLRLSRTVALKETL
                     VETDELRRAFEREARLLANLRHPALPKVLDHFSEGAGTFLAMEYIPGDDLGRMLEERG
                     RSFAPDEVLRWADQLLGALEYLHSLDPPVLHRDIKPSNLMLVSHDQIILLDFGLAKGA
                     AGQMTRTGDGSVLGYSPNYSSLEQIQGAGTDARSDLYALGATVYHLLTNVRPPDALAR
                     ATAVVNGRPDPLRPAHEIDGQVPAAASAALTRALALNIEHRPRAASEMSDDLRGMSLR
                     VAATVPMTDADERTKVSLPAERTGALVPDTARSRERGQRVVVHLPEDTLVVRRQGGGG
                     WKLTLGVGLIAGLVTLSAVLYLSYFGNGKTEPARVNQPAVTSAPSPTPTPVIKSNQQI
                     NSNVGQPVGPTELAARAKLEREGIPYSEEAFNRVVESGDTGAVDLFLAAGMNPDAADA
                     AGRAALIKAASRGRDHISRKLLGGGADVNARGGDGSTALMEASL"
BASE COUNT          264 a          520 c          550 g          280 t
ORIGIN      
        1 agtgaagctt ccatcaaagc cgtcgagccg tcgccgcctc tggcattcac gtccgcgccc
       61 ccgcccaaca gcttccggct gatatggtcg cggcctctgg acgcggcctt gatgagggcc
      121 gccctgccgg cggcgtccgc cgcatccggg ttcatccccg ccgcgaggaa taaatccacc
      181 gcgccggtgt cgccgctttc cacgacgcgg ttgaaagcct cctcgctgta cgggattccc
      241 tctcgctcta acttcgcgcg ggcggccaac tcggtggggc cgaccggctg cccgacgttg
      301 ctattgattt gctgattaga cttgatgacc ggcgtgggtg tcgggctggg ggcgctcgtg
      361 actgccggct gattcacacg cgcgggctcg gtcttgccgt ttccgaagta cgacaggtaa
      421 agcacggcgg acagcgtgac cagcccggcg atcaggccga ccccgagcgt cagcttccaa
      481 ccgccaccgc cttgcctccg cacgaccaat gtgtcctcgg ggagatgaac gactactcgc
      541 tgaccacgct cccgactgcg tgcagtgtcc ggcacgagtg cgccggtacg ctcggcaggc
      601 aaacttacct tggtgcgctc gtccgcgtcc gtcatcggga cggtggccgc gacgcggagc
      661 gacattccgc gcaagtcatc gctcatctcg gaggcggcgc gtggtcggtg ctcgatgttg
      721 agcgccagag cgcgcgtcag ggcggcggag gcggcagccg gcacctgtcc gtcgatctcg
      781 tgggcgggac ggagcgggtc gggccggccg ttcacgacgg cggtcgcgcg ggcgagcgcg
      841 tcgggcggcc tgacattcgt cagcaggtga tagacggtcg ccccgagagc gtagaggtcg
      901 ctgcgcgcgt cggtgcccgc gccctgaatc tgctccaacg acgagtaatt cggggagtag
      961 ccgagcacgc tcccgtcgcc ggtgcgggtc atctgtccgg cggcgccctt cgcgaggccg
     1021 aagtccagca agatgatttg atcatgcgag acgagcatga ggttcgaggg cttgatgtcg
     1081 cggtggagga cgggcgggtc gagcgagtga aggtactcca aagcgcccaa caactgatcc
     1141 gcccaccgca gcacttcgtc gggtgcgaat gaacgccccc gctcttcgag catgcgtccg
     1201 aggtcgtcgc cggggatgta ttccatggcc agaaacgtcc ccgccccctc gctgaaatga
     1261 tccagcactt tcgggagcgc cgggtggcgc aggttggcga gcaggcgcgc ctcgcgctcg
     1321 aaggcgcggc gcaactcgtc cgtctcgacg agggtttcct tgagcgcgac ggtgcggctc
     1381 aggcgtaagt ccgtggcctc gtacaccgct cccatgccgc cccgcccgag cgggcggacg
     1441 acgcggtatc ggccctggag gatggtgtcg gcgacgagca tgaaaatagg gtagtgctga
     1501 aaaggtaatg ctcggtcttt gaaaagagtg gagcgacccg gtgtaatagg aagtgaccaa
     1561 actttctcta cacaggagtc gctccgatga acaacctcag ttgtccacgg tgcg
//
LOCUS       DBNGOH010000234         6844 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_34217, whole genome shotgun sequence.
ACCESSION   DBNGOH010000234 DBNGOH010000000
VERSION     DBNGOH010000234.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6844)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6844)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6844
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_34217"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..112)
                     /locus_tag="AC159P_04380"
     CDS             complement(<1..112)
                     /locus_tag="AC159P_04380"
                     /inference="COORDINATES: protein motif:HMM:NF039182.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5654 family protein"
                     /protein_id="HIR3694721.1"
                     /translation="MKAVNMKVFLQTMITLASASLGLVAALAWNEAIKTTI"
     gene            complement(226..702)
                     /locus_tag="AC159P_04385"
     CDS             complement(226..702)
                     /locus_tag="AC159P_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013570488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HIR3694722.1"
                     /translation="MTEGIKQKLQGELDELESELRVHLPREIKRAKEFGDLRENAEYH
                     AALARQQFVQARIRDLRQRLSEVSSIDLSRVPHDKVAYGSTVVLFDTDSEEEITYRLV
                     TAEESDPDAGLISTTSPVGRSLMGREEGDEVKVVTPSGARRFEIRSLTTLHDQPEG"
     gene            1045..2112
                     /locus_tag="AC159P_04390"
     CDS             1045..2112
                     /locus_tag="AC159P_04390"
                     /inference="COORDINATES: protein motif:HMM:NF024878.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004112 - cyclic-nucleotide
                     phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HIR3694723.1"
                     /translation="MHETPQRGKRKLILFVDDDASLLRLGRLTLERAGYEFLGALGGQ
                     EGLHMARASRPDLILLDYMMPDISGKEVFDELRRSADLRLRRAPVVMLTAHANNEAEK
                     RELLESGLAAYLHKPFGYRELLNVIENVLVLSQIRERNHLLELEARRSFIAMVRTLIT
                     LLFAKDSYTGQHSKAVARLAEGLALHCGLSEEEAMHVKLGALLHDVGKIGVPEGVLCK
                     PDCLTTEELAVMRRHVHHGEQALAGVPRMEALRAMVLHHHEWWDGNGYPAGLCGEEIP
                     LGARIIAVVDAYDAMVSDRPYRDALPRAVAVERLRAGAGTQFDPILVCKLFDYLAKHE
                     AIEQGEIDLEFLEEFCPVVIE"
     gene            complement(2156..2518)
                     /locus_tag="AC159P_04395"
     CDS             complement(2156..2518)
                     /locus_tag="AC159P_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015161441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HIR3694724.1"
                     /translation="MAGKNYLDLIAWQKAMDLVEMVYKATGQFPKDELYGLTNQLRRA
                     AVSIPSNIAEGQGRNSSNDFRRFLAISLGSLREAETQILIAKRLNYLTESQTERLMNL
                     AGEVGRLVNGLSHSLADR"
     gene            complement(2576..3697)
                     /locus_tag="AC159P_04400"
     CDS             complement(2576..3697)
                     /locus_tag="AC159P_04400"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003872 - 6-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008443 - phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HIR3694725.1"
                     /translation="MLKTIALVTGGGDAPGLNAVIRAVVKTAAGEYGLRVVGIEDSFE
                     GLLGEPRLRELTPEHVRGLLPRGGTILGTRNRGRFSRQENGAGQSRRPAEVFAEALDN
                     LKRLSVDALVAVGGEGTLGIAAEFDRKGFPVVGVPKTIDNDLAATELTFGFMTALDAA
                     TWALDRLHTTAESHDRVMVLEVMGRNAGWIALHSGVSGGADVILIPEIPFSMRKVAEK
                     IIERERCDSQFSIVVVAEGAREVEGKEVYQDTGEDDKAPRLGGIGPHVAHELSRQTGK
                     EARCVVLGHLQRGGSPNAFDRMLATNFGSAAVRALTRGERGVMVALQAGNIKTVPIAE
                     AIANIKTVLPTSQLVRTARDVGVSFGAPNEDDFHKHDVC"
     gene            complement(3711..4112)
                     /locus_tag="AC159P_04405"
     CDS             complement(3711..4112)
                     /locus_tag="AC159P_04405"
                     /inference="COORDINATES: protein motif:HMM:NF025444.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4259 domain-containing protein"
                     /protein_id="HIR3694726.1"
                     /translation="MGAWGADSFENDDALDWLADFCDDPEENVILAALRQVADAGDGE
                     YLEAPDCSACVAAAEVVAALKGATRYVPEDVKGCLDASHIKPDEHLVETALRALARVR
                     ADSELKELWDESGGAAEWMEALGDLESRLRR"
     gene            complement(4171..4473)
                     /locus_tag="AC159P_04410"
     CDS             complement(4171..4473)
                     /locus_tag="AC159P_04410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694727.1"
                     /translation="MCHRTVVAVQREIEMRGIPTVLVTVSPEVSAQMRPPRALYPKGF
                     RVGNSLGRPGMRELQRKVLRDALNLLTENTRPGEVVTIDYPEYGEQYEPPRVKAGK"
     gene            complement(4480..4884)
                     /locus_tag="AC159P_04415"
     CDS             complement(4480..4884)
                     /locus_tag="AC159P_04415"
                     /inference="COORDINATES: protein motif:HMM:NF019003.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050485 - oxidoreductase activity, acting
                     on X-H and Y-H to form an X-Y bond, with a disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase
                     selenoprotein B family protein"
                     /protein_id="HIR3694728.1"
                     /translation="MPFTPFEGELARATVGIVSAGGVHLKSQEPFNTADELGDLTFRV
                     VADDVDSAELMVSHHHYDHRDADEDINVVFPIDVLRDLKAEGFVKEVAKKHVGYMGYT
                     MQLKAMYEGTAREIANEIDKGSRADVVVLTGG"
     gene            complement(4999..5463)
                     /locus_tag="AC159P_04420"
     CDS             complement(4999..5463)
                     /locus_tag="AC159P_04420"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF020123.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(5323..5325),aa:Sec)
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HIR3694729.1"
                     /translation="MPEVGDMAPDFELKSHLAPDKKVRLSDFRGQKNVVIAFYPLAWT
                     PVUTAQMPAYEADLERFAGYDAQVLGISVDSVPCNAAWAKSLGGLSYDLLSDFHPKGE
                     VAKAYGAWREADGISERALFIVDKEGRLAYVDIHAIGDQPDNEDLLDVLRKL"
     gene            5702..>6844
                     /gene="pfp"
                     /locus_tag="AC159P_04425"
     CDS             5702..>6844
                     /gene="pfp"
                     /locus_tag="AC159P_04425"
                     /inference="COORDINATES: protein motif:HMM:NF041103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphate--fructose-6-phosphate
                     1-phosphotransferase"
                     /protein_id="HIR3694730.1"
                     /translation="MSTRQVLAILVGGGPAPGINAVISAAAIEAINEGFEVVGVQDGF
                     KWLIREDASKIRPLGIGDVSRIYLKGGSVLGTARDNPTKSDEATRAVVETLRAAAVTH
                     LVTIGGDDTALSASFVAERSNHGISVAHVPKTIDNDLPLPSHIPTFGYQTARHVGVEL
                     VYNLMEDARSTKRWYVVVAMGRKAGFLALGIGKAAGATLTVIGEEFTDRSTIAFSEIC
                     DIVEGAVVKRRASGRNYGVAVLAEGLIEKLDPEELADLQDVERDEHGHIRFAEVDLAR
                     KVKAEVQGRLAGRGIRLTITNKNIGYELRCADPIPFDASYCRDLGYSAIRFLRSGGSG
                     ALISIQGGRMVPIPFADIREPGTGKTRVRHVNIESEGYRVAREYMIR"
BASE COUNT         1093 a         2357 c         2187 g         1207 t
ORIGIN      
        1 cgatcgtcgt cttgatggcc tcgttccacg ccagcgccgc taccagcccc agcgaagccg
       61 acgccagcgt aatcatcgtt tgcaggaaga ctttcatgtt caccgccttc atcctccgcg
      121 caccgcgtct cggtagagct tcccgcggtt tatacgacga cgcgcgcccg acttcaagat
      181 gaacgcggcg cgcgtcgtgg catcggcggt tggcgcaacc gatcttcaac cctcgggttg
      241 gtcgtgcagg gtggtcaggc tccgaatctc gaagcggcgg gcgcctgagg gggtgacgac
      301 tttgacttcg tcgccctctt cgcggcccat caggctgcgc cccacgggcg aggtggtgga
      361 gatgaggccg gcgtcggggt cgctctcttc ggcggtgacg aggcggtagg tgatctcctc
      421 ttcgctgtcg gtgtcgaaga ggacgacggt cgagccgtag gcgactttgt cgtgcgggac
      481 gcgcgagagg tcgatggagg agacttcgct gaggcgctgc cggaggtcgc ggatgcgcgc
      541 ctgcacgaac tgctgccggg cgagcgcggc gtggtactcg gcgttctcac ggaggtcgcc
      601 gaactctttg gcgcgcttga tctcgcgcgg caggtggacg cgcagctccg actccaactc
      661 gtccagttcg ccctggagct tctgcttgat gccttcggtc acttcctttt tccgtccctt
      721 ctgtgggcgc tcgcggacag cgcgcccgtg cgaaatgtaa tcgaaacaca atgcgctgcc
      781 cggcgcgcgc cgggctatac ttcgaccggc gtctcccttc gagtcaaggt caagctccga
      841 cttccgaacg cggtactctc tgccggcttg accctcctcg aaccggacgc cggcggcgct
      901 cgacttgtcc tcttctccct cgcgcgagtg cgctctcttg ctcaagttct aacagatcac
      961 gcacgaccgc attctgtcat aacgatgaag ggggcgcgac ccgactcgcc cgttcgccga
     1021 cgagtcgctc gcggcactga cttgatgcac gagacacctc aacgcggtaa gagaaaactg
     1081 atcctgttcg ttgacgacga cgcctcgctg ctgcgcctgg gccgcctgac gctggagcgc
     1141 gcgggctacg aattcctcgg cgcgctcggc ggccaggagg gcctgcacat ggcgcgcgcc
     1201 tctcgtcccg acctcatcct cctcgattat atgatgcccg acatcagcgg gaaagaggtc
     1261 ttcgacgagc tgcgccggag cgccgacctg cggctccggc gcgcgccggt cgtgatgctc
     1321 accgcgcacg ccaacaacga ggcggagaag cgcgagctgt tggagtcggg gctcgcggcc
     1381 tacctgcaca agccgttcgg ctaccgggag ctactgaacg tgatcgagaa cgtcctcgtc
     1441 ctcagccaga tcagggagcg caaccacctg ctcgaactcg aagcgcgccg ctcgttcatc
     1501 gcgatggtgc gcacgctcat cacgctgctc ttcgccaagg acagctacac gggccagcac
     1561 tcgaaggcgg tggcgcgcct cgccgagggc ctcgcgctgc actgcgggct gtcggaggaa
     1621 gaggcgatgc acgtcaagct cggcgcgctg ctgcacgacg tgggcaagat cggcgtgccc
     1681 gagggcgtgc tctgcaagcc cgactgtctg accacggaag agctggccgt gatgcgccgc
     1741 cacgtccacc acggcgagca ggcgctcgcg ggcgtgcccc gcatggaggc gctgcgcgcg
     1801 atggtcttgc accaccacga gtggtgggac ggtaacggct accccgccgg cctctgcggc
     1861 gaagagatac ctttgggcgc gcgcatcatc gccgtcgtcg atgcctacga cgcgatggtc
     1921 agcgaccgcc cctaccgcga cgcgctgccg cgcgccgtcg ccgtcgagcg cctccgcgcc
     1981 ggcgccggca cgcagttcga ccccatcctc gtctgcaagc tcttcgacta cctcgccaaa
     2041 cacgaagcca tcgagcaggg cgagatcgac cttgagtttc tggaagagtt ctgcccggtg
     2101 gtcatcgaat agtaggcagt aggcggtagg cagttaaagc ttcgtcgagt tgttcctatc
     2161 tatccgcaag cgagtggctg agtccgttga cgaggcggcc cacctctccc gcgagattca
     2221 tcagcctttc ggtctgtgat tcggtcaggt agttcaacct tttggcaatc aggatttggg
     2281 tttcggcttc tctcaacgag ccgaggctga tcgctagaaa ccgccggaag tcgttcgacg
     2341 agttgcgacc ctgaccttcg gcgatgttcg acgggatgga aaccgctgcc cgcctcaatt
     2401 gattcgtcag cccgtagagt tcatccttcg gaaactgccc cgtggctttg tagaccatct
     2461 cgactaaatc catcgccttc tgccacgcga tcaggtcaag gtaattcttc cccgccatcg
     2521 gcactctcct tctagggcgg gttctcgaac tgcctactgc ctactgccta ctctcttaac
     2581 agacatcgtg cttatggaag tcgtcctcgt tcggggcgcc gaaggagacg cccacgtcgc
     2641 gggcggtgcg gacgagctgg ctggtcggga ggacggtctt gatgttggcg atggcttcgg
     2701 cgatggggac ggtcttgatg ttgccggcct ggagcgcgac catcacgccg cgctcgccgc
     2761 gcgtgagggc gcgcaccgcc gccgagccga agttggtggc gagcatgcgg tcgaaggcgt
     2821 tgggcgagcc gccgcgctgg aggtggccga gcacgacgca gcgcgcctcc ttgccggtct
     2881 ggcggctcag ttcgtgcgcc acgtgcgggc cgatgccgcc gagccgcggc gccttgtcat
     2941 cctcgcccgt gtcctggtag acctccttgc cctcgacctc gcgcgcgccc tcggcgacga
     3001 cgacgatgct gaactgcgag tcgcaccgct cgcgctcgat gatcttctcg gcgaccttcc
     3061 gcatcgagaa ggggatttcg gggatcagga tcacgtcggc gccgcccgag acgcccgagt
     3121 ggagcgcgat ccagcccgcg ttgcgcccca tcacttcgag caccatcacg cggtcgtgcg
     3181 actcggcggt ggtgtgcagg cggtcgagcg cccacgtcgc ggcgtcgagc gcggtcatga
     3241 agccgaaggt cagctcggtc gcggccagat cgttgtcgat ggtcttcggc acgccgacca
     3301 cggggaagcc cttgcggtcg aactccgccg cgatgccgag cgtgccctcg ccgccgaccg
     3361 cgaccagcgc gtccaccgac agccgcttca aattgtcgag cgcctcggcg aagacctcgg
     3421 cgggccggcg cgactgaccg gcgccgttct cctgccgcga gaagcgcccg cggttgcgcg
     3481 tgccgaggat cgtcccgccg cgcggcagca ggccgcgcac gtgctccggc gtcagctcgc
     3541 gcaggcgcgg ctcgccgagc agcccctcga agctgtcctc gatgccgacg acgcgcaggc
     3601 cgtactcgcc cgccgcggtc ttcaccaccg cgcggatcac ggcgttcagt ccgggcgcgt
     3661 cgccgccgcc cgtcaccagt gcgattgtct ttagcatccg gcttggcggc tcaccgccgc
     3721 agtctcgatt cgaggtcgcc gagcgcctcc atccactcgg cggcgccccc ggactcgtcc
     3781 cacaactcct tcagctccga gtccgcacgc acgcgcgcca gcgcgcgcag cgccgtctcg
     3841 accagatgtt cgtccggctt gatgtgcgac gcgtcgaggc agcccttcac gtcttccggc
     3901 acgtagcggg tcgcgccctt cagcgcggcg acgacctccg cggcggcgac gcacgccgag
     3961 caatcgggcg cctcaaggta ttcgccgtcg cccgcgtccg cgacctgccg cagcgcggcg
     4021 aggatgacgt tctcctcggg gtcgtcgcag aagtcggcga gccagtccag cgcgtcgtcg
     4081 ttctcgaagc tgtccgcgcc ccaagcgccc atcgcataca cctcccggcc ctgacgcgag
     4141 gtcatgacgc gaggttggtt ggtgcgaggc tcacttcccg gctttgacgc gaggcggctc
     4201 gtactgctcg ccgtactcgg ggtaatcaat cgtcacgacc tctccgggcc gcgtgttctc
     4261 ggtcaggaga ttgagcgcgt cgcgcaagac cttgcgctgc aactcgcgca tgccgggccg
     4321 ccccaggctg ttgcctacac ggaagccctt cgggtagagc gcgcgcggcg ggcgcatctg
     4381 cgccgagacc tcggggctga ccgtgacgag caccgtgggg atgccgcgca tctctatctc
     4441 gcgctgtacc gcgacgaccg tgcggtggca cacgttcggt caaccgcccg tcagcacgac
     4501 cacgtcggcg cgcgaccctt tatcgatctc gttcgcgatc tcgcgcgccg tcccctcgta
     4561 catcgccttg agctgcatcg tgtagcccat gtacccgacg tgctttttgg cgacctcctt
     4621 gacgaagccc tccgccttca agtcgcgcag cacgtcgatc gggaacacga cgttgatgtc
     4681 ctcgtccgcg tcccggtggt cgtagtggtg gtgcgagacc atcagctccg cagagtcaac
     4741 gtcgtccgcc acgacgcgga aagtcaggtc acccaactcg tccgccgtgt tgaaaggttc
     4801 ctgactttta agatggacgc cgcccgccga cacgatcccg acggtcgccc gcgccagctc
     4861 cccctcgaac ggcgtgaacg gcacgcattt tcgtgaaagt gtagtcataa gaacagaggc
     4921 cggaggtcag aggtcggagg tcagtcagaa agcagccagt tttcactgac ccctgacctc
     4981 cgacctctgg cctctgcttc agagtttgcg gagcacatcg agcaagtcct cgttgtcggg
     5041 ctggtcgccg atggcgtgga tgtccacgta ggcgagccgg ccttccttgt caacgatgaa
     5101 gagcgcgcgc tcggagatgc cgtccgcttc gcgccacgcg ccgtaggctt tggcgacttc
     5161 gcccttcggg tgaaagtcgc tgaggagatc gtacgagagg ccgcccaggg atttggccca
     5221 ggcggcgttg cacgggacgc tatcgaccga gatgcccaag acctgggcat cgtagcctgc
     5281 gaagcgttcg aggtctgctt cgtaggccgg catctgcgcc gttcagaccg gcgtccacgc
     5341 cagcgggtag aatgcgatca cgacgttctt ctggccgcgg aaatccgaga ggcgcacctt
     5401 cttgtccggc gcaaggtggc tcttcaactc gaagtccggg gccatgtctc cgacttcagg
     5461 catgcggaga tgtcctcctt cgtcaatgtt gaattcatac tgcgggggta gtgtgacgcc
     5521 tcaaggctac tcccaaccca tgtcgatttc aagacggtgc cgaccgcgcg tataaaaaaa
     5581 acaggggcgc gtattaaaat aatttagccc ggcgcgttaa cgttcgccgc gccgttttct
     5641 gtacaatccg gccttccccg ttcgcgcggc acgaagagga cagtccggcg gaaggcgcat
     5701 catgtcaacc agacaggtct tggcgatact cgtcggcggc ggccccgccc ccggcatcaa
     5761 cgccgtcatc tcggcggcgg ccatcgaggc gatcaacgag ggcttcgagg tcgtcggcgt
     5821 gcaggacggc ttcaagtggc tgatccgcga agacgcctcg aagatacgcc cgctcggcat
     5881 cggcgacgtg tcgcgcatct atctgaaggg cggctcggtg ctcggcaccg cgcgcgacaa
     5941 cccgaccaag tccgacgagg ccacgcgcgc cgtcgtcgag accctgcgcg cggctgccgt
     6001 cacgcacctc gtcaccatcg gcggcgacga cacggccctg tcggcgagct tcgtcgccga
     6061 gcgctcgaac cacggcatca gcgtcgccca cgtgccgaag accatcgaca acgacctgcc
     6121 gctcccctcg cacatcccga ccttcggcta ccagacggcg cgccacgtcg gcgtcgagct
     6181 ggtctacaac ctgatggagg acgcgcgctc gaccaagcgc tggtacgtcg tcgtcgcgat
     6241 ggggcgcaag gccggcttcc tcgcgctcgg catcggcaag gccgcgggcg cgacgctcac
     6301 cgtcatcggc gaagagttca cagaccgctc gaccatcgcc ttctccgaaa tctgcgacat
     6361 cgtcgagggc gccgtcgtca agcggcgcgc ttcgggcagg aactacggcg tcgccgtgct
     6421 cgccgaagga ctcatcgaaa agctcgaccc cgaagagctg gccgacttgc aggacgtgga
     6481 gcgcgacgag cacggccaca tccgcttcgc cgaggtcgat ctcgcgcgca aggtgaaggc
     6541 cgaggtgcag ggccgtctcg cgggtcgcgg catccgcctg acgatcacca acaagaacat
     6601 cggctacgag ctgcgctgcg ccgacccgat ccccttcgac gcctcgtact gccgcgacct
     6661 cggctacagc gcgatccgct tcctgcgctc cggcggctcc ggcgcgctca tcagcatcca
     6721 gggcgggcgc atggtgccca tccccttcgc cgacatccgc gagccgggca cgggcaagac
     6781 gcgcgtccgc cacgtcaaca tcgagagcga gggctaccgc gtcgcgcgcg agtacatgat
     6841 ccgc
//
LOCUS       DBNGOH010000235         4255 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3150310, whole genome shotgun sequence.
ACCESSION   DBNGOH010000235 DBNGOH010000000
VERSION     DBNGOH010000235.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4255)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4255)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4255
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3150310"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2002
                     /locus_tag="AC159P_04430"
     CDS             <1..2002
                     /locus_tag="AC159P_04430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005882 - intermediate filament [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HIR3694731.1"
                     /translation="AGAALAQATYTTGFESPGFTSGDVNGQQGWGHISNSPTKGVIES
                     TPGGSPAAFGAQLLALRTRNVDFFGVSNHLFSATIDPAGETGSTVGGVAVAQPVNHFA
                     ASFHYRTPATPVVSTRADGRFAELDPSSKGAGAEDVANRYAQVRVVNVNNTGNLRFEI
                     GWYTSTSFTFCVQTAAENLTWGAWYRLEYDILFFDGLNGTRPNDVFRFSVYDTNNNLV
                     GAATGSTWEAGWKTGGFGGGTTARAVNGFDLWSQTGPNDALVGLIDNFSMASNNVTFA
                     PADLGSVKISEYRLRGPAGATDEFVELYNNTDAAIIVSDPTPPATGPAGWALVSSDDA
                     GAAKFVVPNGTVIPARGHYLVANSAGYSLSAYPAGYACNVASTATPDDAYTTDIPDLA
                     PGTGGCAAPFTNGRGLALFRTSSPANFTLANRLDAAGSVCETNALFKEGAGHAVLDAG
                     ASGAEHTWFRKLGLTTAGRPQDTGANAADFLFADTAGTSAGAGQRLGAPGPENTSSPV
                     ERNAGLVASLIDPAQPAAAPPNRARDFTSDPANNSAFGTLSVRRRFTNTTGLPVTRLR
                     FRVIDITTFAPAGTADIRARTSADVVVSLTGGGTTTVRGTTLEAPPPAQAHGGGFNSS
                     LSAGVVTLAAPLAPGATIDVQWLLGIQQTGAFRFYINIEALP"
     gene            complement(2174..3166)
                     /locus_tag="AC159P_04435"
     CDS             complement(2174..3166)
                     /locus_tag="AC159P_04435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694732.1"
                     /translation="MKLFRRASLFCALTALLLFAAACSSDMNANKTGANTAATPAATA
                     SPAAQVLGEIPTPQKIRDQMAQRGEQDEAAPTLKVVQPADGSTVNGSTVKVRLTLGGD
                     LKGYKPHKDPATQLGNHIHVILDNQPYEAYYEIDQPFELRNVSEGKHTLRVFPSRPWH
                     ESYKNDGALQMVTFTVKGGGDPAQPTTTNSGQRVADPQNANAAASPAASPAPSPEGKD
                     VQPGTAPGEVDHTKPLLTYSRPKGEYKGADADAIMIDFWLAHGKLQGDGGDYRVRYSV
                     DGGAPKFIDKWQPLWLSGWTAGKHTVKLDLVGKDGQPVENGGYNTTTREITVTK"
     gene            complement(3318..3953)
                     /locus_tag="AC159P_04440"
     CDS             complement(3318..3953)
                     /locus_tag="AC159P_04440"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012525969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HIR3694733.1"
                     /translation="MDSGQIYKPRQFDLSGLNGISDQTLEMHFKLYEGYVKETNNLTS
                     RITEFLKDGKVDQEEMPAYSELTRRLGFEYNGMVLHEYYFENMKKGSTTTDPDKSSAF
                     AKAAEATFGSYDIWKADFVGIGKMRGVGWAICYQNPVNGRLSNHWITLHETGNVSGFA
                     PVLVMDVWEHAFLLDYKPAERPKYIEAFFSNIDWDACEKRLVKEVGQQAGA"
BASE COUNT          679 a         1471 c         1357 g          748 t
ORIGIN      
        1 cgcgggcgcg gccctcgcac aggcgaccta cacgaccggc ttcgagtcgc cggggttcac
       61 gtcgggcgat gtgaacgggc agcagggctg ggggcacatc tcgaacagcc cgacgaaggg
      121 cgtcatcgag tccacgcccg gcggcagccc cgcggccttc ggcgcgcaac tgctcgcgct
      181 gcgcacgcgc aacgtcgatt tcttcggcgt ctcgaaccac ctcttctccg ccaccatcga
      241 cccggcgggc gagacgggca gcaccgtcgg cggcgtcgcc gtcgcgcagc ccgtcaacca
      301 cttcgccgcc tccttccact atcgcacgcc cgcgacgccc gtcgtctcga cgcgcgccga
      361 cggacgcttc gccgaactcg acccgagcag caagggcgcg ggcgcggaag atgtcgccaa
      421 ccgctacgcg caggtgcgcg tcgtgaacgt caacaacacc ggcaacctcc gcttcgagat
      481 cggctggtac acctcgacct ccttcacctt ctgcgtccag accgccgccg agaacctgac
      541 gtggggcgcg tggtacaggc tcgaatacga catcctcttc ttcgacgggc tgaacggcac
      601 gcgcccgaac gacgtgttca ggttttccgt ctacgacacg aacaacaacc tcgtcggcgc
      661 ggccaccggc tcgacgtggg aggccgggtg gaagacgggc ggcttcggcg gcggcacgac
      721 ggcgcgcgcc gtcaacggct tcgacctctg gtcgcagacc ggcccgaacg acgccctcgt
      781 cggcctcatc gacaacttct cgatggcgag caacaacgtc acgttcgcgc ccgccgacct
      841 cggcagcgtg aagatcagcg agtaccggct gcgcggcccc gcgggcgcga ccgacgagtt
      901 cgtcgagttg tacaacaaca ccgacgccgc catcatcgtc agcgacccga cgccgcccgc
      961 gacaggcccc gcagggtggg cgctcgtctc ctcggacgac gcgggcgcgg ccaagttcgt
     1021 cgtccccaac ggcaccgtca tccccgcgcg cggccactat ctcgtcgcca acagcgccgg
     1081 ctactcgctc tcggcctacc ctgccggcta cgcctgcaac gtggcctcga cggcgacgcc
     1141 cgacgacgcc tacaccacgg acatccccga cctcgccccc ggcacaggcg gctgcgccgc
     1201 gcccttcacc aacgggcgcg gcctcgccct cttccgcacc tcgagtccgg ccaacttcac
     1261 gctcgccaac cgcctcgacg ccgccggctc ggtctgcgag accaacgcgc ttttcaaaga
     1321 aggcgcgggc cacgccgtcc tcgacgcggg cgcgtcgggc gccgagcaca cgtggttccg
     1381 caagctgggg ctgacgaccg ccgggcgtcc gcaggacacc ggcgcgaacg ccgccgactt
     1441 cctcttcgcg gacaccgcgg ggacgagcgc gggcgcgggc cagcggctcg gcgcgccggg
     1501 gccggagaac acctcctcgc ccgtcgagcg caacgcgggg ctggtggcct cgctcatcga
     1561 cccagcgcag cctgccgccg ccccgcccaa ccgcgcgcgc gacttcacga gcgacccggc
     1621 gaacaactcg gccttcggca cgctctccgt ccgccgccgc ttcacgaaca ccaccggact
     1681 gccggtcacg cgcctgcgct tccgcgtcat cgacatcacg accttcgcgc ccgcgggcac
     1741 ggctgacata cgcgcccgca cctccgcgga cgtggtggtc tcgctcacgg gcggaggcac
     1801 aaccacggtg cgcggcacga ctttggaggc gccgccgccc gcgcaagccc acggcggcgg
     1861 cttcaactcc tcgctctccg ccggagtcgt caccctcgcc gcgccgctcg cgcccggcgc
     1921 gaccatcgac gtgcagtggc tcctcggcat ccagcagacc ggggccttcc gcttctacat
     1981 caacatcgag gcgctgccgt gaaaacagta ggcagtaggc ggtaggcggt aggcagtaag
     2041 aacagacgag tgaagcggct tgagaggtat cgtgctaagc ctctccgctc tgcaagcaac
     2101 aaagcaagag gcttgagacg ctacgtctca agccacttcc gttttatggt cttgactgcc
     2161 tactgcttac tgcctacttc gtgacggtga tctcgcgggt ggtcgtgttg tagccgccgt
     2221 tctcgacggg ctggccgtcc ttgccgacga ggtcgagctt gaccgtgtgc ttgcccgccg
     2281 tccagcccga gagccagagg ggctgccact tgtcgatgaa cttgggcgcg ccgccgtcca
     2341 ccgaatagcg gacgcggtag tcgccgccgt cgccttgcag cttcccgtgc gcgagccaga
     2401 agtcgatcat gatggcgtcg gcgtccgcgc ccttgtactc gcccttcggg cggctgtagg
     2461 tgaggagcgg cttcgtgtga tcgacctctc ccggcgcggt gcccggctgc acgtccttgc
     2521 cttcgggcga aggggcgggc gaggccgcag gggaagcggc ggcgttcgcg ttctgcgggt
     2581 cggcgaccct ttgcccgctg ttggtcgtgg tcggctgcgc ggggtcgccg ccgcccttga
     2641 cggtgaaggt caccatctgc aaggcgccgt cgttcttgta gctctcgtgc cacgggcgcg
     2701 aggggaagac gcgcagcgtg tgcttgccct cggagacgtt gcgcagctcg aacggctggt
     2761 cgatttcgta gtaagcctcg tagggctggt tgtcgaggat gacgtggatg tggttgccca
     2821 actgcgtcgc cgggtctttg tgcggcttgt agcccttgag gtcgccgccg agggtcaacc
     2881 tgactttgac ggtcgagccg ttgacggtcg agccgtcggc gggctgcacc accttgagcg
     2941 tcggcgcggc ctcgtcctgc tcgccgcgct gcgccatctg atccctgatc ttctgcggcg
     3001 tcgggatttc gccgagcacc tgcgccgcgg gcgaagcggt ggccgcgggc gtggccgcgg
     3061 tgttcgcacc cgtcttgttc gcgttcatgt ccgacgaaca cgccgccgcg aacagcaaca
     3121 gcgccgtgag cgcgcagaag agggacgcac gtcgaaagag tttcattctg tcctccggtg
     3181 tctggaattt ggcatggagt atacccctgt gggaaaggca aaaggcaaga ggcaaaaggc
     3241 aaaaattagc aagcgccaac cgcttttaat ttttgccttt tgcctcttgc cttttgcctc
     3301 ttgccttttg ccttcttcta cgccccggcc tgttggccga cctctttgac gagccgcttc
     3361 tcgcaggcgt cccagtcgat gttggagaag aacgcctcga tgtacttggg ccgctccgcc
     3421 ggcttgtagt cgaggaggaa ggcgtgctcc cacacgtcca tcacgaggac gggcgcgaag
     3481 ccggagacgt tgcccgtctc gtggagcgtg atccagtggt tcgagagccg accgttcacg
     3541 gggttctggt agcagatggc ccagccgacg ccgcgcatct tgccgatgcc gacgaagtcc
     3601 gccttccaga tgtcgtagga gccgaacgtg gcctcggcgg ctttggcgaa ggccgaagat
     3661 ttgtccgggt cggtggtggt cgaacccttc ttcatgttct cgaagtagta ctcgtgcaga
     3721 accatcccgt tgtactcgaa gccgagccgg cgcgtcagct ccgagtaggc gggcatctcc
     3781 tcctgatcga ccttgccgtc cttgagaaac tccgtgatgc ggctcgtcag gttgttcgtc
     3841 tccttgacgt agccctcgta gagcttgaag tgcatttcga gcgtctggtc ggagatgccg
     3901 ttcaatccag agaggtcgaa ctgcctcggc ttataaatct gtccgctgtc catctctact
     3961 gcctcctttg ataagtgcga ggagcaacat tgatttcccg cccccaagtt tactcccgcc
     4021 cgccgcgccg atgtgtggcg aatgagttga gagcgtgagg cattagtgtt gcggacaagc
     4081 ggggaaagaa agggcgaaga ggggaaaagg ggaaagggcg aaaagggaaa atcatgtcct
     4141 ccccttttcg ccctttcccc ttttaacctt ttcccccttc ttcgctattt gccgaagatg
     4201 acccagtcgc cgtgcgaggt ggccatgaag aagagcatcg ggagcgagag ccagg
//
LOCUS       DBNGOH010000236         2260 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2234840, whole genome shotgun sequence.
ACCESSION   DBNGOH010000236 DBNGOH010000000
VERSION     DBNGOH010000236.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2260)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2260)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2260
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2234840"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(93..1457)
                     /locus_tag="AC159P_04445"
     CDS             complement(93..1457)
                     /locus_tag="AC159P_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR3694734.1"
                     /translation="MNTLSKHKLKLLISSAVCLTALLVLVAAGRSGKSEEAAAAAPPV
                     GVEVARVERRDVPIHSEWIGTTDGMNNAEIKAQVAGYLLSKNYTEGSLVRKGQLLFEI
                     DPRPLQAALDQARGKLAQAQGQVAQAASQLSQAEAQVAQAASQHSQADAQVLQAEANQ
                     GKTQRDVNRTTALVKDGVVSREEYDNAVQANLAARAQVAAARAGVRAAAAQVQAARAQ
                     VKTVGSVIVSARAQVQAAQAEVRTAELNLGFTRIISPIDGIAGIAKAQVGDLVSPAGD
                     ALTTVSTLDPIKVYFTLSEQEYLGFTRRSPTPQRWDAANRSLELELVLADGTTYPERG
                     RFYVADRQVDERTGAIRLAGIFSNPGNTLRPGQYGRVRAVTSVKEGALLVPQRAVTEL
                     QGSYQVAVVGADNKVGMRTVKVGERVGTMWIIESGLQPDERVVAEGTQKVRPGAAVNP
                     QPFN"
     gene            complement(1849..>2260)
                     /locus_tag="AC159P_04450"
     CDS             complement(1849..>2260)
                     /locus_tag="AC159P_04450"
                     /EC_number="5.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047661 - amino-acid racemase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HIR3694735.1"
                     /translation="PLSPIPWLHIARAVAEEAHRRGYARLGILGTKYLAEGPVYPVAL
                     EEFKIESRIPDEADREKINEIIFKELVNGIFTEDSRSYFNEVASKLKARGCDAVVLGC
                     TEIPLIVRPDDTPLPTLDSTRLLARAALRKALEQ"
BASE COUNT          322 a          772 c          734 g          432 t
ORIGIN      
        1 gtgcggattc gtgatggatg agagccgcgc tgtcatccat cacgaatccg ccttcactct
       61 ttaatccgca atccccaatc cgcaatccgc aatcagttga acggctgcgg gttgaccgcc
      121 gcgccgggtc tgactttttg cgtgccctcg gcgacgaccc gttcgtcggg ttgcaacccg
      181 ctctcgataa tccacatcgt cccgacgcgc tcgccgacct tgacggtgcg catgccgacc
      241 ttgttgtcgg cgcccacgac ggcgacctga tagctgccct gcaactccgt gacggcccgc
      301 tgcgggacga gcagcgcgcc ctccttcacg ctggtcacgg cgcgcacccg cccgtactgg
      361 ccggggcgca gcgtgttgcc ggggttcgag aagattccgg cgagccggat cgcgcccgtc
      421 ctctcgtcca cctgccggtc ggcgacgtag aacctgcccc gctccgggta ggtcgtcccg
      481 tcggcgagga cgagttccag ttcgaggctc cggttcgccg cgtcccacct ttgcggcgtc
      541 gggctgcgcc gtgtgaagcc gagatactcc tgctcgctga gggtgaaata gaccttgatg
      601 gggtcgaggg tcgagaccgt ggtcagcgcg tcgccggcag ggctgaccag atcgccgacc
      661 tgcgctttgg cgatccccgc gatgccgtca atcggcgaga tgatccttgt gaaaccgaga
      721 ttcagctccg ccgtcctgac ctcggcctgt gctgcctgca cctgcgcccg ggccgagacg
      781 atcaccgacc cgaccgtctt gacctgcgca cgggccgcct gcacctgtgc ggctgccgca
      841 cggactcccg ccctcgcggc ggcgacttgc gcccgcgccg cgaggttggc ctgcacggcg
      901 ttgtcgtatt cctcgcgcga gaccacgccg tccttcacga gcgcggtggt gcggttcacg
      961 tcgcgctggg tcttcccctg attcgcctcg gcctgtaaga cctgcgcgtc ggcctgcgag
     1021 tgctggctcg cggcctgcgc gacctgcgcc tcggcttgcg agagttggct cgcggcctgc
     1081 gcgacctgcc cctgcgcctg cgcgagtttg ccgcgggcct gatcgagcgc cgcctgcaac
     1141 ggcctcgggt cgatctcgaa aagcaactgg cccttcctga ccagagagcc ttccgtgtag
     1201 ttcttgctga gcaggtagcc cgccacctgc gccttgatct ccgcgttgtt catcccatcg
     1261 gtcgtgccga tccactcgct gtgaatcggc acgtcccgcc gctcgacccg cgcgacctcg
     1321 acgccgaccg ggggcgccgc cgccgcggcc tcttcggact ttccggagcg cccggcggca
     1381 accaacacga ggagcgcggt caggcagacc gccgacgaga tcagtaactt gagcttgtgc
     1441 ttcgacagcg tgttcatcct cttctccgat tcaaggacga gggccggcga caggatcacg
     1501 gagggacttc gatgtcgctc cgccgagtcg tctttaccgc atcagtccgg ccatgctttc
     1561 gtgcaagcgc cctgccacac ttcgacgaag acgcgcaggc ccgcgtcaag tagttgatac
     1621 tcatcggtaa gtgttcgacg tggcggcgag aacacgacgg cccggcggag acggggcgcg
     1681 ggattcgcgg cgacatatcg cttcggatgc gatatgtcgc cgccggcgac atccgtctcg
     1741 gccaacggcg gcggcggctt ggcctgtcgt cagtggccgt ctgcttcggc ggtgatggag
     1801 gggatgcggt tggtgtctgc gtcgaggccg cgtcgggtgc gagcctgctt attgctcaag
     1861 ggccttccta agggccgctc gcgcgagcag gcgtgtcgaa tccaaagtgg gcagaggggt
     1921 gtcgtccggg cgcacgatca gaggtatctc ggtgcagccg aggacgacgg cgtcacagcc
     1981 gcgcgccttc agcttgctcg ccacttcgtt gaagtacgaa cgggagtctt ccgtgaagat
     2041 gccgttgacc aactccttga agatgatctc gttgatcttt tccctgtccg cctcgtcggg
     2101 tatccggctc tctatcttga attcctccag cgccaccggg tagacaggcc cttccgccag
     2161 atacttggtc cctagaatgc cgagccgcgc gtagcccctg cggtgggctt cttcggcgac
     2221 cgcgcgggcg atgtgcagcc acgggatggg ggacaggggg
//
LOCUS       DBNGOH010000237         7005 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_767729, whole genome shotgun sequence.
ACCESSION   DBNGOH010000237 DBNGOH010000000
VERSION     DBNGOH010000237.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7005)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7005)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7005
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_767729"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            81..1406
                     /locus_tag="AC159P_04455"
     CDS             81..1406
                     /locus_tag="AC159P_04455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694736.1"
                     /translation="MSKRNGPSKASGLVAAAAAAALLLPLGFTNGAAQSAARRATRPA
                     VVNPKTAAVRAATAEVLKETSEIRQLPVLRPVRSGAQSRAEIEQMLVRNLNENTTPEE
                     LRASERTLKKLGLVPDDFQLRPFMLRLLVEQVAGYYDPRTKGFYLADWIDLDGQRPVM
                     AHELTHALQDQHFNLRRFEKWPEHDSDAELAAHALIEGDAMVLMTQYVVKSPARQLAM
                     IRSFLTGESGSTEQFDKAPRVLRESLLFPYTQGSVWAGQVYKRGGWEAISRAYTELPA
                     STEQILHPEKYFAGEAPSKLAFKDVSAVLGRGWQVADHDVNGEWGYFLVLDEFLKARD
                     ASQTAAAGWHGDRLALYLGPTGGDVLLTHQTAWDTEQDAAEFFDAYARRTTARYKVEP
                     SADGATPRRRLWATGEGSVLMELSGKSVLIVEGAPDAAKAEAVVRALAK"
     gene            1775..3418
                     /locus_tag="AC159P_04460"
     CDS             1775..3418
                     /locus_tag="AC159P_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HIR3694737.1"
                     /translation="MKSETAPASSGRGTRLRELTAELRALEARLREGGGADKIERQHK
                     QGKLTARERIALLLDRETYAQEIGLLVAYDQYEGGAPAAGVVTAVGRVGGREVVVVAN
                     DATVKAGSWWPETIKKILRAQEIAMRSRVPIIYLVDSAGVNLPFQGGVFPGQYGAARI
                     FYYNSIMRRYLHVPQVAAVMGPCVAGGAYLPALSDIIVMVEGTSFMGLGGANLVKGAT
                     GQTIDNETLGGARTHNEISGVAHYRVETDEACLSKIREIVSELPSNPKALVSINESRE
                     AARPGEDLYAIIPEDHRHPYDAREMLRCILDEGHLDEFQAGYAKEMIVGHARVHGIQV
                     GVIANNRGMIRNPAGGPPRFGGIIYTESAEKVAYFIDVCNRQRTPLLFIQDVSGFMIG
                     ADAEHSGIIRAGARFVEAMATAAVPKIVLTVNHASGAGYYAMAGQGFDPDFIFSWPTG
                     RMGVMEGDSAVQAIYGTQLEKLKREGREPDEKLKDEMARVRDDYEHQLDARYAAARGF
                     VDAVLAPEETRDALDLALRVTLNFDGPHLGQFVLPASPV"
     gene            3533..4132
                     /locus_tag="AC159P_04465"
     CDS             3533..4132
                     /locus_tag="AC159P_04465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694738.1"
                     /translation="MDRKLSANRIALAALAAFALLALCVADLGALAQNANSSTTADPS
                     MENHNMMTHAPARRRRGSRRRPRPAAEATDASMAADASAAQEERTDGGEQADLSGTYA
                     GRMTMRGSHDMAGEATLTITGNTFTLAGEGMNHSGRVLAVTTRGYTGASFFFSDLSDA
                     DTKTPVVAAVRARKTARGLTLTPVPGTRTRLTFNGRSSS"
     gene            4266..5642
                     /locus_tag="AC159P_04470"
     CDS             4266..5642
                     /locus_tag="AC159P_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyclic terpene utilization AtuA family protein"
                     /protein_id="HIR3694739.1"
                     /translation="MDKRREMIRVASGQGFWGDMLDAPVRQVEGGPIDYLMLDYLAEV
                     TMSIMQKQRARDPAAGYARDFVPLMREILPACVERNIRVTANAGGVNPEGCAAAVGEV
                     ARELGLAGRLRIGTVTGDDVLQRLDDFIERGVELRNMDTGEPLSTVRERVQSANVYLG
                     AWPIVEALNRGAQVVVTGRATDTGLTLAPIIHEFGWREDDWDRLAAGTIAGHIIECGA
                     QASGGNCQYDWESIPDLAGVGFPIVEARADGTFVVTKHEGTGGRVSVPIIKEQLLYEM
                     GDPREYITPDCVADFTTIRLEDAGADRVRVHGIKGTPATEFYKVSISYSAGFKAVGTL
                     VYAWPDAYRKARAADRILRERLERLGLRFDHVLTEFVGANATHGPLAGEPSPDHPEIQ
                     LRFGVRDQDRKAVERFTKEIAPLILTGPPAVTGFAGGRPKTEEIVAYWPALIPKTEIT
                     PRVEVTEA"
     gene            5639..6211
                     /locus_tag="AC159P_04475"
     CDS             5639..6211
                     /locus_tag="AC159P_04475"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006423133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR3694740.1"
                     /translation="MSDPSELRWRRRRTERWADCRVFTVRRDINVSPRDGREHDFYVI
                     EAPDWINVIPLTANEEVVMIEQYRFGTDEVTLEIPGGMVDAGETPREAAAREMLEETG
                     FAPVELIALGKTRPNPAIQNNWIHTFLARGAEFRHDPAFDGTEHTIVRLVPLARIPSL
                     IADGTITHSLVVVGFHWLSLFKEGALKEAM"
     gene            6214..6540
                     /locus_tag="AC159P_04480"
     CDS             6214..6540
                     /locus_tag="AC159P_04480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694741.1"
                     /translation="MDESFSVLIIDMYHYDPAEDYVIGGFPTFELAKEFARRWVRDSV
                     EELRRPGQSNDELRRQWFMFGEDASVLRGEPHYAGSHELDYFVAHPATRDERDWQAVK
                     TLAGIH"
     gene            6564..6908
                     /locus_tag="AC159P_04485"
     CDS             6564..6908
                     /locus_tag="AC159P_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtuA-related protein"
                     /protein_id="HIR3694742.1"
                     /translation="MHIQLLELAHARSGDKGDTANVGLIALREEFYPLLVREVTAERV
                     KRHFEGICKGEVERFELPNLAALNFLLHESLGGGGTLSLMTDAQGKTFSTALLRMEID
                     VPDEEARELNIT"
BASE COUNT         1221 a         2357 c         2397 g         1030 t
ORIGIN      
        1 cgccgacggg gctggtcttc ggaaagagtt agggtgcgag ccgcctgttt ctggcggact
       61 ataagagcga aaggttcaag atgtcaaaga ggaatgggcc gtcgaaggct tccgggctgg
      121 tggccgccgc ggctgccgcc gcgctgctcc tgccgctcgg cttcacgaac ggcgccgcgc
      181 aaagtgccgc gcggcgggcg acgagacccg cggtcgtcaa cccgaagacg gcggcggtgc
      241 gcgcggcgac cgccgaagtc ctgaaggaga cgagcgagat caggcagctc ccggtgctgc
      301 ggcccgtccg cagcggcgcg cagtcgcgcg cggagatcga gcagatgctc gtccgcaacc
      361 tcaacgagaa cacgacgccc gaagagctgc gggcgagcga gcggacgctg aagaagctcg
      421 gcctcgtccc cgacgacttc cagctccggc ccttcatgct ccgcctgctg gtcgagcagg
      481 tcgcgggcta ctacgacccg cgcacgaagg ggttctacct cgccgactgg atcgacctcg
      541 acggccaaag gcccgtcatg gcgcacgagc tgacgcacgc cttgcaggat cagcacttca
      601 acctgcgccg cttcgagaag tggcccgagc acgactcgga tgccgagctg gcggcgcacg
      661 cgctcatcga gggcgacgcg atggtcttga tgacgcagta cgtcgtcaaa agcccggcgc
      721 gccagctcgc catgatccgc tcgttcctga cgggcgagtc cggctcgacc gagcagttcg
      781 acaaggcgcc gcgcgtcctg cgcgagtcgc tgctgttccc ctacacgcag ggcagcgtgt
      841 gggccgggca agtttacaag cgcggcggct gggaggcgat ctcgcgcgcc tacacggagc
      901 tgcccgcctc gaccgagcag atcctccacc ccgaaaaata tttcgcgggc gaggcgccgt
      961 cgaagctcgc cttcaaggac gtttccgccg tgctcggcag gggatggcag gtggccgacc
     1021 atgacgtgaa cggcgagtgg ggctacttcc tcgtcctcga cgaattcctg aaggcgcggg
     1081 acgcctcgca gaccgccgcc gcgggctggc acggcgaccg cctcgcgctc tacctcggcc
     1141 ccacgggcgg cgacgtcctc ctcacgcacc agaccgcgtg ggacaccgaa caggacgccg
     1201 cagagttttt cgacgcctac gcgagacgca ccacggcgcg ctacaaagtc gagccttcag
     1261 ccgacggagc gacgccccgc cgccgcctct gggcgaccgg cgaaggcagc gtgctgatgg
     1321 agttgagcgg caagagcgtc ctcatcgtcg aaggcgcgcc cgacgcggcg aaggccgagg
     1381 cggtcgtgag ggcgttggcg aagtagctgc aagaatgaag cgcgcacgtc gccgcggagc
     1441 ggcggttgag tttagcccgg cctttcaagg ccgggcaccg gcgcaccgtt cggtgttgcg
     1501 tcgcgtcagc gacgcgtgaa tgcgcggcgt tcgttcaggc gtcgctgacg cgacgcggcg
     1561 ccctctcgcc gacctttccc ggcgttgaaa cgccgggcta cattcataat gccgctacgc
     1621 ggcaaatgac attcagtcgt gcagagaccc gcgttgtttt cgcgcgcgcg tctgtgatat
     1681 tttcaaatca aattcaagct ctgagatttc cattaacagc cggtcacttc gcgcgccgcg
     1741 gtgcgcggcg cggggggccg gtgtggggtc gtgaatgaag tcagaaacgg caccggcttc
     1801 ttccgggagg ggcacgcgcc tgcgcgagct gacggcggag ctgcgcgcgc tcgaagcccg
     1861 tctgcgcgag ggcggcggcg cggacaagat cgagcgccag cacaagcagg gcaagctgac
     1921 ggcgcgcgag cgcatcgcgc tgctgctcga ccgcgagact tacgcgcagg agatagggtt
     1981 gctcgtcgct tacgaccagt acgagggcgg cgcccctgcg gcaggcgtgg tgacggcggt
     2041 ggggcgcgtc ggcgggcgcg aggtggtggt cgtcgccaac gacgcgacgg tgaaggccgg
     2101 ctcgtggtgg cccgagacga tcaagaaaat cctgcgcgcg caggagatcg ccatgcgctc
     2161 gcgcgtgccg atcatctatc tggtcgattc ggcgggtgtg aacctcccct ttcagggcgg
     2221 tgtctttccg ggccagtacg gcgcggcgcg catcttctac tacaactcga tcatgcgccg
     2281 gtatctgcac gtcccgcagg tcgcggcggt gatggggccg tgcgttgcgg gcggcgcgta
     2341 cctgcccgcg ctctcggaca tcatcgtgat ggtcgagggc acctcgttca tgggactcgg
     2401 cggcgcgaac ctcgtgaagg gcgcgaccgg ccagaccatc gacaacgaga cgctcggcgg
     2461 cgcgcgcacg cacaacgaga tttccggcgt cgcgcattac cgtgtcgaaa cggacgaagc
     2521 ctgcctgtcg aagataagag agatcgtgtc cgagctgcct tcgaacccga aggctctggt
     2581 ctcgatcaac gagagcaggg aggcggcgcg gcccggggaa gacttgtacg cgatcatccc
     2641 cgaagaccac cgccaccctt acgacgcgcg cgagatgctg cgctgcattc tggacgaggg
     2701 gcacctcgac gagtttcagg cgggttatgc caaagagatg atcgtcggcc acgcgcgcgt
     2761 gcacggcata caggtcggcg tcatcgccaa caaccgcggg atgattcgca acccggcggg
     2821 cggcccgccg cgcttcggcg gcatcatcta cacggagtcc gccgagaagg tcgcctattt
     2881 catcgacgtg tgcaaccgcc agcgcacgcc gctcctcttc atacaggacg tgtccggctt
     2941 catgatcggt gcggacgccg agcacagcgg gatcatccgc gcgggcgcgc gcttcgtcga
     3001 ggcgatggcc accgccgccg tgccgaagat cgtcctgacg gtcaatcacg cctcgggcgc
     3061 gggctactac gcgatggcgg ggcagggctt cgaccccgac ttcatcttca gttggccgac
     3121 gggcaggatg ggcgtgatgg agggcgactc ggcggtgcag gccatttacg ggacacagct
     3181 cgaaaagctg aagcgcgaag gcagagagcc ggacgagaag ctgaaggacg agatggcgcg
     3241 cgtgcgcgac gactacgagc accagctcga cgcgcgctac gccgccgcgc gcggcttcgt
     3301 ggacgccgtc ctcgcgcccg aagagacgcg cgacgcgctg gacctcgcgt tgcgcgtgac
     3361 gctgaacttc gacgggccgc acctcggtca gttcgtgctg cccgcgtcgc cggtctagag
     3421 tccgagcctt cgggggggga cgcactgcta ccgtatgtcg ttaaccactt cgccgccgcg
     3481 cgccgcaggg gcattccccc gcgcggcggc ttaaaccgga ggagaaaaca ccgtggacag
     3541 aaagctaagt gctaaccgga tcgccctggc ggcgttggca gccttcgcgc tcctcgccct
     3601 ctgcgtcgcg gacttgggag cgctcgcgca gaacgctaac agcagcacga ccgccgaccc
     3661 gagcatggag aaccacaaca tgatgacgca cgcgcccgcc cgccgccggc gcggcagccg
     3721 acgccgcccg cggcccgccg cggaagcgac cgacgcgagc atggccgccg acgcctccgc
     3781 ggcgcaggag gagcgcaccg acggcggcga gcaggccgac ctgtccggca cctacgcggg
     3841 ccgcatgacc atgcggggca gccacgacat ggcgggcgag gcgacgctca ccatcaccgg
     3901 caacacgttc acgctggcgg gcgaggggat gaaccacagc ggtcgcgtcc tcgccgtcac
     3961 gacgcgcggc tacacaggcg cttcgttctt cttcagcgac ctgtccgacg cggacacgaa
     4021 gacgcccgtc gtcgccgccg tgcgcgcgcg caagaccgcc cgcggcctga cgctgacgcc
     4081 cgtgcccggc acgcgcaccc gcctgacgtt caacggacgg agcagcagct aagacgggcg
     4141 cgtccgccgt tccgccgaga gcgagttcgg agtccggccc gaagctgctt cgcgccgggc
     4201 cacggcccga aggccgagct ccacggctcc cgtcaacgag acgccggaca cggcccgatt
     4261 attttatgga taagagaaga gagatgatcc gcgtcgccag cggtcagggc ttctggggcg
     4321 acatgctcga cgcgcccgtg cggcaggtcg agggcgggcc gatagattac ctgatgctcg
     4381 actacctggc cgaggtcacg atgtcgatca tgcagaagca gcgcgcgcgc gaccccgccg
     4441 ccggctacgc gcgcgacttc gtgccgctca tgcgcgagat tctgcccgcg tgcgtcgagc
     4501 gcaacatccg cgtgacggcg aacgcgggcg gcgtcaaccc ggaggggtgc gccgccgccg
     4561 tcggcgaggt ggcgcgcgaa ctcggactcg cgggccgtct gcgcatcggc accgtcaccg
     4621 gcgacgacgt gttgcagagg ctcgacgact tcatcgagcg aggggtagag ctgcgcaaca
     4681 tggacacggg cgagccgctc tcgaccgtgc gcgagcgcgt tcagagcgcc aacgtctatc
     4741 tcggcgcgtg gccgattgtc gaggcgctga acaggggcgc gcaggtcgtc gtcacgggcc
     4801 gcgcgaccga cacgggcctc acgctcgcgc ccatcatcca cgagttcggc tggcgcgaag
     4861 acgactggga caggctggcc gccggaacca tcgcgggcca catcatcgag tgcggcgcgc
     4921 aggcgtcggg cggcaactgc cagtacgatt gggagtcgat acccgacctc gcgggcgtcg
     4981 gcttccccat cgtcgaggcg cgcgccgacg gcaccttcgt cgtcaccaag cacgaaggca
     5041 cgggcggtcg cgtctccgtg ccgatcatca aggagcaact gctctacgag atgggcgacc
     5101 cgcgcgagta catcacgccc gactgcgtcg cggacttcac gaccatccgc ctcgaagacg
     5161 cgggagccga ccgcgtccgc gtccacggca tcaagggcac gcccgcgacc gagttctata
     5221 aagtctcgat cagctactcg gcgggcttca aggcggtcgg cacgctcgtc tatgcgtggc
     5281 ccgacgcgta ccgcaaggcg cgggccgccg acaggattct gcgcgagcgg ctcgaacggc
     5341 tcggcctgcg gttcgaccac gtgctgaccg agttcgtcgg cgccaacgcc acgcacggcc
     5401 cgctcgccgg cgagccttcg cccgatcatc cggagattca actgcgcttc ggcgtgcgcg
     5461 atcaggacag gaaggcggtc gagcgcttca ccaaagagat cgcgccgctc atcctgacgg
     5521 ggccgcccgc cgtcacgggc ttcgcgggcg gacggcccaa gacggaagag atcgtcgcct
     5581 actggcccgc cctcatcccg aagaccgaga tcacgccgcg cgtggaggtg acggaggctt
     5641 gagcgacccg agtgagctga ggtggcgacg gcggcgcacg gagcggtggg cggactgccg
     5701 cgtcttcacc gtccgcaggg acatcaacgt cagcccgcgc gacgggcgcg agcacgactt
     5761 ctacgtcatc gaggcgcccg actggatcaa cgtcatcccg ctcaccgcca acgaagaggt
     5821 cgtgatgatc gagcagtacc gcttcggcac ggacgaggtg acgctcgaaa tccccggcgg
     5881 catggtggac gcgggcgaaa cgccgcgcga ggccgccgcg cgcgagatgc tggaagagac
     5941 gggcttcgct cccgtggaac tcatcgcgct cggcaagacg cgaccgaacc ccgcgatcca
     6001 gaacaactgg attcacacct tcctcgcgcg cggcgcggag ttccggcacg accccgcctt
     6061 cgacgggacg gagcacacta tcgtccgcct cgtgccgctc gcacgcatcc cctcgctcat
     6121 cgccgacggc accatcacgc actcgctcgt cgtcgtcggc tttcactggc tctcgctttt
     6181 caaggagggg gccttaaaag aagcgatgta gtcatggacg agagcttctc ggttctcatc
     6241 attgacatgt accattacga cccggcggaa gattatgtca tcggcggctt ccccacgttc
     6301 gaactggcga aggagttcgc gcgccgctgg gtgagggatt cggtcgagga gcttcgccgg
     6361 cccggacagt cgaacgacga actgcgaagg caatggttta tgttcggcga ggacgcctcc
     6421 gtcctccggg gcgagccgca ctacgccggc tcgcacgagc tggattactt cgtcgcgcac
     6481 cccgccacgc gcgacgagcg cgactggcag gccgtcaaga cgctggcggg cattcactag
     6541 gcgtccgatc cgattcgaag gcaatgcaca tccaacttct cgaacttgct cacgcgcgct
     6601 ccggcgacaa gggcgacacg gccaacgtcg gactcatcgc gttgcgcgaa gagttctatc
     6661 cgctcctcgt gcgcgaggtg acggcggagc gcgtcaagcg ccacttcgag ggcatctgca
     6721 agggcgaggt cgagcgcttc gagctgccga acctcgcggc gctcaacttc ctcctgcacg
     6781 agagtctcgg cggcggcggc acgctctcgc tgatgaccga cgcgcagggc aagaccttct
     6841 cgacggcgct gctcaggatg gagatcgacg tgcccgacga agaagcccgt gaattaaaca
     6901 taacttgagt ctgatacatg acgacccgcg gttgaagctg caactccgcg taagcaagat
     6961 gctggggtac gggtttgcct tctcgttaat ttgggccggc ggggt
//
LOCUS       DBNGOH010000238         2438 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_767818, whole genome shotgun sequence.
ACCESSION   DBNGOH010000238 DBNGOH010000000
VERSION     DBNGOH010000238.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2438)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2438)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2438
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_767818"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..783)
                     /locus_tag="AC159P_04490"
     CDS             complement(<1..783)
                     /locus_tag="AC159P_04490"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010581705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3694743.1"
                     /translation="MVTDQIRFDDGAAYERYMGAWSRLAGETFLDWLAPQPGLRWLDV
                     GCGNGAFTEMLVERCAPASVHGIDPSEGQLAYARTRPAARVAQFRQGDAMAQPFPDDT
                     FDVAVMPLVIFFVPEPARGVAEMARVVCPGGVVTAYAWDMEGGGFPYEALQEEMRGMG
                     VAVPVPPSPDASGIDALRDLWAGAGLDSVETREITVRRTFADFEDYWTTVLGGPSVGP
                     QLAAMAFEGLALLKARMRARLPVDAVGSITYGARANAVKGRVP"
     gene            complement(1110..2411)
                     /locus_tag="AC159P_04495"
     CDS             complement(1110..2411)
                     /locus_tag="AC159P_04495"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HIR3694744.1"
                     /translation="MKRPTFSDTELHTLQRNTFNYFWKETNPRNGLLADNTSGGVPAS
                     IAAVGHALAACAVGVERGWVTRGDAVRRTLATLRFFRDSPQGKQPDATGYKGFYYHFL
                     DIETGRRAWKSELSTIDTTILLAGVLTAAAYFDQDTAEEREVRAVASELYRRVDWRWA
                     QNGGRAVAHGWKPERGFLKYRWEGYNEALILYVLGLGSPTHALPAESYAAWTETYRWK
                     KLYGIEFLYAGPLFVHQLSHIWIDFRGIQDDFMRRHAIDYFENSRRASYVQQQYAVRN
                     PRGFRGYDEYCWGITASNGPGPAVLKVGGVRRRFHAYRARSVPFGLDDGTLAPWATVA
                     SLPFAPEIVLPTLAVFKEKWGEMLSRYGIACSFNPTFPSKENRHAGWVSGGHFGIDQG
                     PVILMIENYRSGLLWRLMRRCPHIVRGLSRAGFGGGWLGTR"
BASE COUNT          412 a          839 c          774 g          413 t
ORIGIN      
        1 cgggacgcgc cccttcaccg cgttagcccg tgcgccatag gtgatactgc cgacggcatc
       61 caccggcagg cgcgcgcgca tccgcgcttt gaggagcgcg agaccttcga acgccatcgc
      121 cgcgagctgt gggccgacgc tcggcccccc gaggacggtc gtccagtagt cttcgaaatc
      181 ggcgaacgtc cgtcgcacgg tgatctcccg cgtctcgacc gaatccagac ccgcgccggc
      241 ccacaagtcc cgcagcgcgt ctattccgga cgcgtcaggg cttggcggca ccgggaccgc
      301 aacgcccatc ccgcgcattt cctcctgcaa cgcctcgtaa gggaaaccgc cgccttccat
      361 atcccacgcg taggccgtga cgacgccgcc ggggcagacc acccgcgcca tctcggcgac
      421 gcccctggcc ggctccggga cgaagaagat caccagcggc atgacggcca cgtcgaacgt
      481 gtcgtcgggg aagggctgcg ccatcgcgtc gccctgacgg aactgtgcga cacgcgccgc
      541 gggtcgtgtg cgcgcgtagg cgagctgccc ctcggacggg tctatcccgt gtaccgaggc
      601 cggcgcacac cgctcgacga gcatctcggt gaaggcgccg ttgccgcagc ccacgtcgag
      661 ccatcgcaga cccggttgcg gggcgagcca gtcgaggaaa gtttcacccg cgagccggct
      721 ccacgccccc atgtatcgtt cgtaggcggc cccgtcgtcg aagcggattt ggtcggtcac
      781 cattagtgtt acctctgatc gggccacctg cgaaacgtgg tggtatatcg cggtgtcttg
      841 tgccgcgtaa agcatagtgg gcgtactgac gaccgccggt aaagacgttg ttcgggcgcc
      901 tagctcttaa tacgccgggc tgcttgctaa tcgtctgcta ttaactgtag agcactgtcg
      961 cgcctccgag ttcggttata aggcttcttc actgagacgt acccgtcgcc ctcgcggtta
     1021 atgagaagcc ccgccgccgg acccggaagt ccggcccctg caatcgccag ccagtcgcgg
     1081 ttcatgctcg ccctttcacc tcaacgcatc taacgagtcc ccagccagcc gcctccgaat
     1141 cctgcgcggc tgaggccacg gacgatgtgc gggcagcgcc gcatcagccg ccagagcagg
     1201 cccgaacggt agttttcgat catcaaaatc acaggcccct gatcgatccc gaagtggccg
     1261 cccgaaaccc agcccgcgtg tcggttctct ttgctcggga aagtcggatt gaaactgcaa
     1321 gcgatgccgt accggctcag catctcgccc cacttctctt tgaagacggc gagcgtcggc
     1381 agcacgatct ccggcgcgaa ggggagcgag gcgaccgtgg cccacggcgc gagcgtgccg
     1441 tcgtcaaggc cgaaggggac gctgcgcgcg cgataggcgt ggaagcgcct tcggacgccg
     1501 cccaccttca gcacggcggg gccggggccg ttcgaggccg tgatgcccca gcagtactcg
     1561 tcgtagccgc ggaagccgcg cgggttgcgg acggcgtact gctgctgcac gtaggaggcg
     1621 cggcggctgt tctcgaaata gtcgatcgcg tggcggcgca tgaagtcgtc ctgaatgccg
     1681 cggaagtcta tccagatgtg agagagctgg tggacgaaga gcggcccggc gtacaggaac
     1741 tcgatgccgt agagcttctt ccagcgataa gtctccgtcc aggccgcgta actctcggcg
     1801 gggagcgcgt gcgtcggaga acccagcccg agcacgtata gaatcaacgc ctcgttgtag
     1861 ccctcccaac ggtatttcag aaatccgcgc tccggcttcc agccgtgcgc gaccgcccgc
     1921 ccaccgttct gcgcccaacg ccagtcaacc cgccggtaaa gctcggaggc taccgcgcga
     1981 acctcccgct cctccgccgt gtcctggtcg aagtaagcgg cggcggtcag cacgccggcc
     2041 aagagtatcg tcgtgtcaat cgtcgaaagc tcgctcttcc acgcgcgccg cccggtctcg
     2101 atgtcgagga agtgatagta aaaacctttg tagccggtcg cgtcgggctg cttgccctgc
     2161 gggctgtcgc ggaagaagcg caacgtcgcc agcgtccgcc tgacggcatc gccgcgcgtg
     2221 acccacccgc gctcgacgcc gaccgcgcag gcggccagcg cgtggccgac ggcggcgatg
     2281 ctggccggca cgcccccgct cgtgttatcg gccagcagac cgttacgggg gttggtctcc
     2341 ttccagaagt agttgaaagt gttacgttga agcgtatgca gctctgtgtc gctgaacgtc
     2401 ggtcgcttca ttcgtgctca cctccggccc tcggttcg
//
LOCUS       DBNGOH010000239         3018 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4800799, whole genome shotgun sequence.
ACCESSION   DBNGOH010000239 DBNGOH010000000
VERSION     DBNGOH010000239.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3018)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3018)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3018
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4800799"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2866)
                     /locus_tag="AC159P_04500"
     CDS             complement(<1..2866)
                     /locus_tag="AC159P_04500"
                     /inference="COORDINATES: protein motif:HMM:NF051698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR3694745.1"
                     /translation="MHYAISFPRALLKRFFTLSALLLCTALAAHAQTTSTLTGEARDA
                     TGALVAGVAVTAKSLETGAVRSVTSDGEGRYTFPGLPVGAYEVRAKKEGFLEYVSERV
                     NLTVNETATLDFTLQVRGLEGEAVTVTDDAALVNTQTQELSYLVGERTIRELPLNGRN
                     YTDLAFLQPGVVAYPHRDGGSVVAHGVGMSINGQDPRSNVYLIDGTPQNDFTNGPAGS
                     AAGTSLGTEAIREFRVETNAYSAEFGRNAGGQINAVTKSGTNDWRGSLYEFHRNDNLD
                     ARNFFDGARKPEFKRNQFGGQIGGPLAKDKLFLFFNYESLRENLGKTVQTVVPDLNAR
                     QGILPGRPVVAVSPVIRPYLDEFPLPNGANLGGGLAAYSFGFAQRLRQHFLQGRVDYN
                     RSERDQFFARYTFDKADQFLPTDFPQFPRTFLSRNQFFTGEYRQTLSASTLNTFRFSF
                     SRTRIGQTVESNVSPNLTPFTPGEERIGSIDIAGIPRFGTQSSADLNLVQNVWGGEYQ
                     VVHSRGRHLIKAGALAERYQDNMVNPTFSLGLFTFASLENFLLNRAQRFIGITPGGAL
                     DRYWRYTLVGLYAQDTYRAHQRLSLNAGVRYEFTTMPVDINGRDSSLPSLTDRTPTSG
                     PLYENPSLKNISPRVGFAWDVFGDGKTSLRGGYGIYYNTNNQQNLIVTVTNPPATPRP
                     VVANPAFPVPNFNGSLSIRPVQFDLDNPYLHVYNLNLQRELWFDTVMTVGYAGSRGVH
                     LLRSGDVNIPTPVIRADGTPFWAAGLPRPNTAFTTIELKSSDGNSWYNALVFELRKRF
                     SKGITAQSSYTFSRNIDTTQASTFFSDGTNGTTSAFPEFPGLNYNKGLADFHAKHNWV
                     VNFTWQIPYARGMDGLKGALLDGWQLAGIGQARSGNPLTVFIQANRSRSQWSPSLAPG
                     VGFDRPNMAPGRTYDDAVLGRPDQYFDPTAFVLQPEGTLGN"
BASE COUNT          460 a          863 c         1150 g          545 t
ORIGIN      
        1 cgttgccgag cgtgccttcg ggttgcagga cgaaggcggt cgggtcgaag tactggtcgg
       61 gccgaccgag gacggcgtcg tcgtaggttc tgccgggcgc catgttcggg cggtcgaagc
      121 cgacgccggg ggcgagcgag ggcgaccact gcgagcgcga gcggttggcc tggatgaaga
      181 cggtcagggg gttgccgctg cgggcctgcc cgatgcccgc gagctgccag ccgtcgagga
      241 gcgcgccctt gaggccgtcc atcccgcgcg cgtaaggaat ctgccacgtg aagttgacga
      301 cccagttgtg cttggcgtgg aagtcggcga ggcccttgtt gtagttgagg ccggggaact
      361 ccgggaaggc ggaggtcgtc ccgttcgtcc cgtcggagaa gaaggtcgaa gcctgcgtcg
      421 tgtcgatgtt gcgcgagaag gtataggagg actgcgccgt gatgcccttg ctgaagcgct
      481 tgcgcagctc gaagacgagc gcgttgtacc acgagttgcc gtcggaagat ttcagctcga
      541 tggtcgtgaa ggccgtgttc gggcgcggca gaccggcggc ccagaagggc gtgccgtcgg
      601 cgcggatgac aggcgtgggg atgttcacgt cgccgctgcg caagaggtgg acgccgcgcg
      661 agccggcgta gccgaccgtc atcacggtgt cgaaccacag ctcgcgctgg aggttcaggt
      721 tgtagacgtg caggtagggg ttgtcgaggt cgaactggac ggggcggatc gagagcgagc
      781 cgttgaagtt cggcaccggg aacgcggggt tggcgacgac ggggcgcggc gtcgcgggcg
      841 ggttcgtgac ggtgacgatc aggttctgct ggttgttcgt gttgtagtat atcccgtagc
      901 cgccgcgaag gctggtcttg ccgtcgccga acacgtccca cgcgaagccg acgcgcgggc
      961 ttatgttctt gagcgagggg ttctcgtaga gcgggccgga ggtcggcgtg cggtcggtca
     1021 aggaaggcag cgaagagtcg cgcccgttga tgtccacggg catcgtcgtg aactcgtagc
     1081 gcacgcccgc gttgaggctc aggcgctggt gcgcgcggta ggtgtcctgc gcgtagaggc
     1141 cgacgagcgt gtagcgccag tagcggtcga gcgcgccgcc gggcgtgatg ccgatgaagc
     1201 gctgcgcgcg gttgaggagg aagttttcga ggctggcgaa ggtgaagagg ccgaggctga
     1261 aggtcgggtt gaccatgttg tcctggtagc gctcggcgag cgccccggcc ttgatgaggt
     1321 ggcgaccgcg gctgtggaca acctgatact cgccgcccca gacgttctgc acgaggttga
     1381 ggtcggcgga agactgcgtg ccgaagcgcg ggatgccggc gatgtcgatg ctgccgatgc
     1441 gctcctcgcc cggcgtgaag ggcgtcaggt tcggcgagac gttcgactcg accgtttgac
     1501 cgatgcgcgt gcggctgaag ctgaagcgga aggtgttcag cgtcgaggcc gagagcgtct
     1561 gccgatactc gcccgtgaag aactggttgc gcgaaaggaa cgtgcgcggg aactgcggga
     1621 agtcggtcgg caggaactgg tcggccttgt cgaaggtgta gcgcgcgaag aactggtcgc
     1681 gctcgctgcg gttgtaatcg acgcgccctt gcaggaagtg ctggcggagc cgctgggcga
     1741 agccgaagct gtaggccgcc agaccgccgc ccagattggc gccgttcggc agcgggaact
     1801 cgtcgaggta ggggcggatg accgggctga ccgcgacgac gggacgaccg ggcaggatgc
     1861 cctgacgggc gttgaggtcg ggcacgaccg tctgcacggt cttcccgagg ttctcgcgca
     1921 aagattcgta gttgaagaag aggaagagct tgtcctttgc gagggggccg ccgatctggc
     1981 cgccgaactg gttgcgcttg aactcgggct tgcgcgcgcc gtcgaagaag ttgcgcgcgt
     2041 cgaggttgtc gttgcggtgg aactcgtaga ggctcccgcg ccagtcgttg gtgcccgact
     2101 tggtgacggc gttgatctgc ccgcccgcgt tcctgccgaa ctcggcgctg taggcgttgg
     2161 tctcgacgcg gaactcgcgg atcgcttcgg tgccgagcga ggtgccggcg gcgctccccg
     2221 ccgggccgtt ggtgaagtcg ttctgcggcg tgccgtcgat gaggtagacg ttcgagcgcg
     2281 ggtcctgccc gttgatgctc atgccgacgc cgtgcgcgac caccgagccg ccatcgcggt
     2341 gcgggtaggc gacgacgccg ggctggagga aggcgaggtc tgtgtagttc ctgccgttca
     2401 agggcagctc gcggatggtg cgctcgccga ccagatagct cagctcctgc gtctgcgtgt
     2461 tgacgagcgc cgcgtcgtcc gtgacggtga cggcctcgcc ttcgaggccc cgcacctgga
     2521 gcgtgaagtc gagcgtcgcc gtctcgttca ccgtcaggtt cacgcgctcc gagacgtatt
     2581 cgaggaagcc ctccttcttg gcgcgcacct cgtaggcgcc gacgggcagg cccgggaaag
     2641 tgtagcgccc ttcgccgtcg ctcgtgaccg agcgcacggc gcccgtctca aggctcttgg
     2701 ccgtcacggc gacgcccgcg acgagcgcgc ccgtcgcgtc gcgcgcctcg cccgtgagcg
     2761 tgctggtggt ctgcgcgtgg gccgcgagcg ccgtgcagag gaggagggcc gaaagtgtaa
     2821 aaaacctctt gaggagcgcg cgcgggaaac tgattgcgta atgcatcttg attcttatta
     2881 ctccgtagtg gcggacttgc agtgccggtt ggcgccgtgc attctaacag gcgtcggcga
     2941 ggttttcgta ggagcgccgc ccgcttttac aaacctttga cgactggcgc gcttctttta
     3001 caccttcttt acaacatc
//
LOCUS       DBNGOH010000240         4979 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1685826, whole genome shotgun sequence.
ACCESSION   DBNGOH010000240 DBNGOH010000000
VERSION     DBNGOH010000240.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4979)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4979)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4979
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1685826"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..381
                     /locus_tag="AC159P_04505"
     CDS             <1..381
                     /locus_tag="AC159P_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type sigma factor"
                     /protein_id="HIR3694746.1"
                     /translation="EVRWQNRAHFFAVSAQLMRRILVDFARARGYQKRGGGAPRVTLD
                     DGIIGSDDKGTDLVALDEALHALAELDARQSRVVELRFFGGLNIDETAEVLKVSPGTV
                     RRDWSLARAWLHRELSKTVTGDER"
     gene            442..3459
                     /locus_tag="AC159P_04510"
     CDS             442..3459
                     /locus_tag="AC159P_04510"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR3694747.1"
                     /translation="MTPERYRHVGELYHAALELDADARAAFLERACADDPALRREVES
                     LIASHEQASDFIAEPALAVAAGLLAEREDAALVGRTIARYRVLSLVGAGGMGRVYLAE
                     DTGLGRRVALKLLPAHFTHDKEQVRRFRQEARAASALNHPNILTVHEVGEADGTEFIA
                     TEYVEGETLRACLARARCGVGEALDVAAQIADALVAAHEARIVHRDIKPENVMLRRDG
                     YVKVLDFGLAKLTESLTAPPTPEPEAATRSVVRTNPGVVMGTAEYMSPEQARGLAVDT
                     RTDVWSLGVVLYEMVAGRRPFGGETHGDMIVSILEREPAPLLRHAPAAPAELERIVRK
                     ALAKDREERYQTVKGMATDLKRLRRRLEAEAEIEHSGQAALGGDAARATGSGGRRAAV
                     TAVQAGAPISAAGAARTTSSVEYLVSQIGRHKRRAALVLGALVLLLGGAGYGLYRLAN
                     RLPPPAPPFQTVKLTRLTATGKVKDAAISPDGKYVVYMEEEARRQSLWVRQVATGSSV
                     PLVPPADVIYTGLTFSNDSNYVYYVKGTRDEPGWPVYQVPSLGGASKKVIERVDSAVA
                     FSPDGRRFAFVRALPQQGESTLVIVNADGTGERALATRKTPDFFLYEGIVRLAWSPDG
                     KMIACPAGSADAGGLFYNVVGVRVEDGATERLTTQRWGWVSQVAWLADGSGLVMVAGE
                     EEVSAPQLWRLSYPGGEARRITNDLHRYTDVSLTGDSRSLVTVRTNRASNIWVAPEGD
                     AARARQITAGTSDSSLGLSWTPDGRIVYQTDASGKPEIWAVNADGSDPRQLTHDGYNY
                     RPATSPAGRYIVFHSFRAGRTNIWRMDVDGGNPKQLTDGQRNLFPAISPDGQWVVYAS
                     PDAGDITLWKVPIDGGDPVRLHETIANLPAISPDGKYVASFYWDERANPPQGVMILPA
                     AGGEPVKRFDILPDAVNGFALHWSPDGRALLHFRDDLSNIWSQPVDGGKPTQATNFQG
                     DQIFNFAWSHDGKQLALARGRVTDDVVLISDAR"
     gene            3747..4943
                     /locus_tag="AC159P_04515"
     CDS             3747..4943
                     /locus_tag="AC159P_04515"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017321634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HIR3694748.1"
                     /translation="MTTPASTKHGRVSQKASRFTESVIREMTREAIKHGAVNLAQGFP
                     DFPAPEEIKRAAIEAIDADVNQYAITWGAKDFRDAIAEKTKWYLGLDIDPEREITVTC
                     GSTEGMIAAMMATVDPGEEVVIFEPFYENYAPDAILSDATPRYVSLRAPDWTFDPEEL
                     RAAFNERTRAVIVCNPNNPTGKVFTREEMEQIAALCREFDALCFTDEIYEHILYPRAD
                     REVEHISMAQLEGMRERTVVVNSMSKTYSVTGWRVGYCLAPPDITGAVRKVHDFLTVG
                     AAAPLQRAGAYALRLPRDYYDHLQRDYRRRRDMLLPVLEDAGFKVFAPDGAYYIMTDI
                     SAFGFADDTEFTRHLIRDIGVACVPGSSFYSRPELGARQVRFCFCKKDETLEAAAERL
                     RRLQKH"
BASE COUNT          836 a         1748 c         1668 g          727 t
ORIGIN      
        1 gaggtgcgct ggcagaaccg cgcgcacttc ttcgccgtct ccgcgcagct catgcgccgc
       61 atcctcgtgg acttcgcgcg cgcgcgcggc tatcagaagc gcgggggcgg ggcgccgcgg
      121 gtcacgctcg acgacggaat catcgggtcg gacgacaagg gcaccgatct ggtcgcgctc
      181 gacgaggcgc tccacgcgct cgccgaactc gacgcgcggc aaagccgggt cgtcgagctg
      241 cggttcttcg gcggcctgaa catcgatgag accgccgagg ttttgaaggt gtcacccggc
      301 acggtgaggc gcgactggag cctcgcccgc gcatggctcc accgtgaact ctccaagacc
      361 gtgacggggg atgagcgctg agacacgtgc ctcgtctctc cagcgctcat cgcccatcac
      421 tcgacgccca acactttcct gatgacgccc gaacgctaca ggcatgtcgg cgagctgtac
      481 cacgccgcgc tggagttgga cgcggacgcg cgcgccgcct tcctcgaacg ggcgtgcgcc
      541 gacgaccccg cgctgcggcg cgaggtcgag tcgctgatcg catcgcacga gcaggcttcg
      601 gacttcatcg cggagccggc gctcgcggtc gccgccgggc tgctcgccga gcgcgaggac
      661 gccgcgctcg tggggcggac aatcgcccgc taccgtgtgc tgtcgctcgt cggcgcgggg
      721 ggcatgggcc gggtctacct ggccgaggac actggcctgg gccgccgcgt cgccttaaag
      781 ctcctgccgg cacacttcac ccacgacaag gagcaggtgc ggcgcttccg gcaggaggcg
      841 cgcgccgcct cggccctcaa ccacccgaac atcctgaccg tccacgaggt cggcgaggcc
      901 gacggcacgg agttcatcgc caccgagtac gtcgagggag agaccttgcg cgcttgtctg
      961 gcgcgcgccc gctgcggcgt cggcgaggcg ctcgacgtgg cggcgcagat cgccgacgcg
     1021 ctcgtcgccg cgcacgaggc gcgcatcgtc caccgtgaca tcaaacccga gaacgtcatg
     1081 ctccggcgcg acggctatgt gaaggtgctc gacttcggtc tcgccaagct gacggagagc
     1141 ctgacggcgc ccccgacgcc cgagcctgag gccgcgacga ggtcggtcgt gcggactaac
     1201 cccggcgtgg tgatgggcac cgccgagtac atgtcgcccg agcaggcgcg gggcctcgcc
     1261 gtggacacgc gcaccgacgt gtggagcctc ggcgtcgtac tctacgagat ggtcgcgggg
     1321 cggcgcccgt tcgggggcga gacccacggc gacatgattg tttcgatctt ggagagggag
     1381 ccggcgccgc ttctgcgaca cgctcccgcg gccccggcag aactggaacg gatcgtccgg
     1441 aaggcgctgg cgaaagacag ggaggaacgc taccagacgg tcaagggcat ggcgaccgat
     1501 ctcaagcggc tccggcggcg gctggaggcc gaggccgaga tcgaacactc cgggcaagcc
     1561 gcgttgggcg gcgacgcggc gcgcgccacg ggcagcggcg ggcggcgcgc ggcggtgacc
     1621 gccgtgcagg caggggcgcc gatcagcgcc gccggggcgg cgcgcaccac gtcgagtgtg
     1681 gaatacctcg tcagccagat cgggagacac aagcggcggg cggcgctcgt cctcggcgcg
     1741 ctcgtgctgt tgttgggcgg cgccggctac ggcctgtacc gactcgccaa ccgcctgccg
     1801 ccgcccgccc cgccttttca aacggtgaag ctgacgaggc tgaccgcgac cggcaaggtt
     1861 aaggacgcgg ccatctcgcc cgacggcaag tacgtggtct acatggagga ggaggcccgg
     1921 cggcaaagcc tctgggtcag acaggtggcg acgggcagca gcgtaccgct cgtcccgccc
     1981 gccgacgtca tttacaccgg cctcaccttc tcgaacgaca gcaactatgt gtattacgtg
     2041 aaggggacga gggacgagcc ggggtggccg gtctatcaag tgccctcgct gggcggcgcc
     2101 tcgaagaagg tgatcgagcg cgtagacagc gccgtggcct tctcccccga cggtcggcgc
     2161 ttcgccttcg tgcgcgccct gccccaacag ggggaaagca cgctggtcat cgtcaacgcc
     2221 gacggcaccg gagagcgcgc gctcgccacg cgcaagacgc ccgacttttt cttgtacgag
     2281 gggatcgtca ggctcgcctg gtctcccgac ggaaagatga tcgcctgccc cgccggcagc
     2341 gccgacgcgg ggggactctt ctacaacgtc gtcggcgtgc gggtcgaaga cggcgcgacg
     2401 gagcggctga ccactcaaag gtgggggtgg gtctcgcagg tcgcgtggct ggccgacggc
     2461 agcggtttgg tcatggtggc cggggaggaa gaggtctcgg ctccgcaact gtggcgactc
     2521 tcctatcccg gcggcgaggc gcggcgcatc accaacgact tgcaccgtta caccgacgtg
     2581 agcctgaccg gagattcccg gtctctcgtc accgtccgga cgaatcgcgc ctcgaacatc
     2641 tgggtcgcgc ccgaaggcga cgccgcccgc gcccggcaga ttaccgccgg cacgtccgac
     2701 agttcgctgg gtctctcgtg gacgcccgac ggcaggatcg tctatcagac agacgcgagc
     2761 ggcaagccgg agatatgggc ggtgaacgcg gacgggagcg acccgcgaca actgacgcac
     2821 gacggttaca actaccgccc ggcgacttcg cccgccgggc ggtacatcgt cttccactcc
     2881 ttccgcgcgg gccggaccaa catctggcgc atggacgtgg acgggggcaa cccgaaacag
     2941 ttgaccgacg gccagcgaaa cctcttcccc gccatttcgc cggacgggca gtgggtggtt
     3001 tacgcgtcac ccgatgcggg cgacatcacc ctgtggaaag tgcccatcga cggcggcgac
     3061 cccgtgcgcc tgcacgagac gattgcgaac ctgccggcca tctcacccga cgggaaatac
     3121 gtcgccagct tctactggga cgagcgggcg aacccgccgc agggggtcat gatcttgccc
     3181 gccgccggag gcgaacccgt gaagcggttc gacatcctgc ctgacgccgt caacgggttc
     3241 gccctgcact ggtcgccgga cgggcgcgcg ctattgcact tccgcgacga tctctccaac
     3301 atctggagcc agcccgttga cggcgggaag ccgacgcagg cgaccaactt tcaaggcgat
     3361 cagatcttca atttcgcctg gtcgcacgac ggcaaacagc tcgccctggc gcgaggccgc
     3421 gtgaccgacg acgtggtgct catcagcgat gccagatgag ccggcgggcg gcaggccgtc
     3481 agcagtgggc tgtcgcaggg aacggctctt tactgcgcac ggctgacgac ccgccgctca
     3541 cgcctcttca cagcagaccg agtttgttcg tcaggaagat gaggacgaag gcgagcgcca
     3601 ggaacgtcgc cagccacggg tgtttgccca tcgggcactt gccgtcttct ttcattgtca
     3661 ctctccggtt gcccgcggcg cgctccgtgt gcgagcatca cgcctcgttc ggcaatttta
     3721 tttcacgccc gcgatggtga atttacatga ccacgccagc ctctaccaaa cacggacggg
     3781 tctcgcagaa ggcgagccgc ttcacggagt cggtcatccg cgagatgacg cgcgaggcga
     3841 tcaaacacgg cgccgtcaac ctcgcgcagg gctttccaga cttccccgcg cccgaagaga
     3901 tcaagcgcgc cgccatcgag gccatcgacg cggacgtgaa ccagtacgcg atcacgtggg
     3961 gcgcgaagga cttccgcgac gccatcgcgg agaagacgaa gtggtatctc ggactcgaca
     4021 tcgaccccga gcgggagatc accgtcacct gcggctcgac cgaaggcatg atcgccgcga
     4081 tgatggcgac ggtcgatccc ggcgaggagg tcgtcatctt cgagccgttc tacgagaact
     4141 acgcgcccga cgccatcctc tccgacgcca cgccgcgcta cgtcagcttg cgcgcgcccg
     4201 actggacgtt cgaccccgaa gagctgcgcg ccgccttcaa cgagcgcacg cgcgccgtca
     4261 tcgtctgcaa ccccaacaac ccgacgggga aggtcttcac gcgcgaagag atggaacaga
     4321 tcgccgcgct ctgccgcgag ttcgacgcgc tctgcttcac cgacgaaatc tacgagcaca
     4381 tcctctaccc gcgcgcagac cgcgaggtcg agcacatctc gatggcgcag ctcgaaggca
     4441 tgcgcgagcg cacggtggtc gtcaactcga tgtcgaagac ctactcggtc acgggctggc
     4501 gcgtcggcta ctgcctcgcc ccgcccgaca tcaccggcgc ggtgcgcaag gtgcacgact
     4561 tcctgacggt cggcgccgcc gcgcccctgc aacgcgcggg cgcttacgcc ttgcggctgc
     4621 cgcgcgacta ctacgaccac ctgcaacgcg actaccggcg ccgccgcgac atgctcctgc
     4681 ccgtcctcga agacgcgggc ttcaaggtct tcgccccgga cggcgcctac tacatcatga
     4741 ccgacatctc cgccttcggc ttcgccgacg acaccgagtt cacgcgccac ctcatccgcg
     4801 acatcggcgt cgcctgcgtc cccggctcca gcttctacag ccgccccgaa ctcggcgcgc
     4861 gccaggtgcg cttctgcttc tgcaagaagg acgagacgct cgaagcggcc gccgagcgcc
     4921 tgcgccggct tcagaagcac tgaccgcgcc ccgccccgcg tgcatccacg cggcggagg
//
LOCUS       DBNGOH010000241         3439 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_768695, whole genome shotgun sequence.
ACCESSION   DBNGOH010000241 DBNGOH010000000
VERSION     DBNGOH010000241.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3439)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3439)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3439
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_768695"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..704)
                     /locus_tag="AC159P_04520"
     CDS             complement(<1..704)
                     /locus_tag="AC159P_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HIR3694749.1"
                     /translation="MPTYVFKGRNRLGEVIVGERMADSREVLRQLLRREQVILTSIKE
                     KGREIAVPKLGRRKKVKAKELAIFTRQFSVMIDAGLPLVQCLDILSQQQENKFFAQVL
                     VQVRQDVEEGSTLAAAMSRHGKVFDQLFVNMIEAGETGGILDLILQRLSTYIEKIVKL
                     RRDVVSALIYPSAVIVIAVGVIAVIMVFVIPAFQNIFLGLLGPGEQLPLPTRIVVGVS
                     DFLAGLGGLCILAGLVG"
     gene            complement(835..2034)
                     /locus_tag="AC159P_04525"
     CDS             complement(835..2034)
                     /locus_tag="AC159P_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HIR3694750.1"
                     /translation="MSANMEPQPEPISLSDLLKKLIEVGGSDLHLTTNTAPQVRVDGH
                     LRPLDGIRPLTSADTKSLAYSVLTDAQKHRFEEQLELDFSFGVRGLSRFRANLFNQRG
                     AVGAVFRAIPYEIKSFETLGLPPVVSTLCDRPRGLILVTGPTGSGKSTTLAAMLDKVN
                     RERHEHIVTIEDPIEFLHSHKSCIVNQREVGADTKGFAEALRTALRQDPDVVLVGEMR
                     DLETIESALRIAETGHLTFATLHTNTAASTINRVIDVFPAEQQSQIRAQLSMVLEGIL
                     CQSLLPRADGRGRAMGLEILVPNAAIRNLIREDKVHQIYSMMQTGQDKYGMQTFNQAL
                     ATLYHKRIITLDIALQRSSNPEELRELIEHGAGINNGGSGGAQRPGAHPSPYAQARPI
                     GARPNAR"
     gene            complement(2143..2979)
                     /locus_tag="AC159P_04530"
     CDS             complement(2143..2979)
                     /locus_tag="AC159P_04530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694751.1"
                     /translation="MTNLFKNLRPLVLSLLIFALVAGGTPVTLETFAKWGRPSAVRRK
                     KNIKRYRRHSRAWWRRYRARQKARRERAERRRLERQASLSARNTSTGTASGSTTRPVN
                     HSGANSALAVSAPRLPFDVQLPHTWTGGRKGASGEVTFAVRGADGRAAGTAVLLPVAV
                     SAADAPDAVGPRTKTIGGIPVAALRRTVIDRMVAAGGWVVNDMVREMGGRRVFVVLAH
                     TGTPGAPTKSLTFYFAEVDGRVYSLATAAPVELAEPIAAGSEQFMSTLRAAGSRNVAS
                     QQ"
     gene            complement(2985..>3439)
                     /locus_tag="AC159P_04535"
     CDS             complement(2985..>3439)
                     /locus_tag="AC159P_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HIR3694752.1"
                     /translation="STLHTNDAPSTISRLMNMGIEPFLVATSVNLIQAQRLIRRICAD
                     CKVEQNLPPEALAEVGFSPAEAKTMKTYKGQGCTKCNDTGYKGRIGLYEVMEVTDEVR
                     ELILIGASALELRKRAIEDGMITLRESGLQKIRNGVTTLEEVVRETVA"
BASE COUNT          510 a         1028 c         1304 g          597 t
ORIGIN      
        1 ccgacgaggc cggcgaggat gcagaggccg ccgaggccgg cgaggaagtc gctgacgccg
       61 acgacgatgc gggtcgggag cgggagctgc tcgccggggc cgagcaggcc gaggaagatg
      121 ttctggaagg ccgggatgac gaagaccatg atgacggcga tgacgccgac cgcgatgacg
      181 atcaccgccg acgggtagat cagcgccgag acgacgtcgc gcctgagctt gacgatcttc
      241 tcgatgtagg tcgagagccg ctggaggatg aggtcgagga taccgccggt ctcgcccgcc
      301 tcgatcatgt tgacgaagag ctggtcgaag accttgccgt ggcgcgacat ggccgcggcg
      361 agggtcgatc cctcctccac gtcctgccgc acctgcacga gcacctgcgc gaagaacttg
      421 ttctcctgct gctgcgagag gatgtcgagg cactgcacga gcggcaggcc ggcgtcgatc
      481 atgacggaga actggcgcgt gaagatggcc agctccttgg ccttcacctt cttgcgcctg
      541 ccgagcttgg gcaccgcgat ctcgcgcccc ttctccttga tcgaggtcag gatcacctgc
      601 tcgcgccgca ggagctggcg cagcacctcg cggctgtccg ccatccgctc gccgacgatc
      661 acctcgccga gccggttgcg gcctttgaag acgtatgtgg gcatctctcg ttacctcaaa
      721 agacgtgaac cgtgaaccgt caaccgtgac gagtggggag ccgtgatgag tgaaccgtga
      781 cgagtgaaga gcttgtttta acttgtcacg gttcactcat cacggcttac ggccttatcg
      841 ggcgttgggc cgggcgccga tggggcgggc ctgtgcgtag ggcgaggggt gtgcgccggg
      901 gcgctgggcg ccgccgctgc cgccgttgtt gatgccggcg ccgtgttcga tcagctcgcg
      961 cagctcttcg gggttggagg agcgctggag cgcgatgtcg agcgtgatga tgcgcttgtg
     1021 gtagagcgtg gcgagcgcct ggttgaaggt ctgcatcccg tacttgtcct ggccggtctg
     1081 catcatcgag taaatctggt gcaccttgtc ctcgcggatg aggttgcgga tggcggcgtt
     1141 ggggacgagg atttcgaggc ccatcgcgcg cccgcgcccg tccgcccgcg gcagcaagga
     1201 ctggcagagg atgccttcca agaccatcga gagttgcgcg cggatctgcg actgctgctc
     1261 ggcggggaag acgtcgatga cgcggttgat ggtcgaggcg gcggtgttgg tgtggagcgt
     1321 ggcgaaggtg aggtggccgg tctcggcgat gcggagcgcc gactcgatgg tttcgaggtc
     1381 gcgcatttcg ccgacgagca ccacgtccgg gtcttgacgc aaggcggtgc gcagggcttc
     1441 ggcgaagccc ttggtgtcgg cgccgacttc gcgctggttg acgatgcagc tcttgtgcga
     1501 gtggagaaac tcgatggggt cttcgatggt gacgatgtgc tcgtggcgct cgcggttgac
     1561 cttgtcgagc atggcggcga gcgtggtgga tttgccggag ccggtcgggc cggtgacgag
     1621 gatgaggccg cgcgggcggt cgcagagcgt cgagacgacg ggcggcaggc ccagcgtttc
     1681 gaaggacttg atctcgtaag ggatggcgcg gaagacggcg ccgaccgcgc cgcgctggtt
     1741 gaagaggttg gcgcggaagc gcgagaggcc gcgcacgccg aaggagaagt cgagttcgag
     1801 ctgctcctcg aagcggtgct tctgcgcgtc ggtcaggacg ctgtaggcga gcgacttggt
     1861 gtcggcggag gtgagcgggc ggatgccgtc gagggggcgc aggtggccgt caacgcgcac
     1921 ctgcggcgcg gtgttcgtcg tcaggtgcaa gtccgagccg ccgacctcga tcagcttctt
     1981 cagcaagtcc gagagcgaaa tcggctccgg ctggggttcc atgtttgcag acatgctctt
     2041 tcctcttcgg cgcggcgcgg gatgagggag tcgcttcggg gggggcgcgc gcggtggtcg
     2101 atggttttat ctcaaagcgg acgaagctca ggggtgaggt ctctactgtt gcgaggcgac
     2161 gttgcggctg cccgccgcgc ggagcgtgga catgaactgc tcggagccgg cggcgatcgg
     2221 ctcggccaac tcgacgggcg cggcggtggc gaggctgtag acgcgcccgt caacctccgc
     2281 gaagtagaac gtcagggact tggtcggcgc gcccggcgtg cccgtgtgcg cgagcacgac
     2341 gaagacgcgg cgaccgccca tctcgcgcac catgtcgttg accacccagc cgcccgcggc
     2401 gaccatccgg tcgatgaccg tgcggcgcag cgcggcgacg gggatgccgc cgatggtctt
     2461 cgtgcgcggg ccgacggcgt cgggcgcgtc cgcggcggag acggcgaccg gcaggagcac
     2521 ggcggtgccc gccgcgcgac cgtcggcgcc gcgcacggcg aaggtgactt cgccgctcgc
     2581 gcccttgcga ccgcccgtcc aggtgtgcgg tagctgcacg tcgaagggga gacgcggcgc
     2641 gctcacggcc aacgcgctgt tggcgccgct gtgattgacg gggcgcgtag tggagccgct
     2701 ggccgtgccc gtcgaagtgt tgcgcgcgga gagcgacgcc tgccgctcaa gcctccggcg
     2761 ctcggcgcgc tcgcgccgcg ccttctgccg cgcgcggtag cggcgccacc acgcgcgcga
     2821 gtggcggcgg tagcgcttga tgttcttctt gcggcgcacg gcggaggggc ggccccactt
     2881 ggcgaacgtc tccagggtga cgggcgtgcc gcccgcgacc agcgcgaaga tgagcaggga
     2941 gaggacgagg ggccgcaggt ttttgaagag attcgtcatg gggttcatgc caccgtctcg
     3001 cgcaccactt cttcgagcgt ggtgacgccg ttcctgatct tttgcaggcc ggactcgcgc
     3061 agcgtgatca tgccgtcctc gatggcgcgc ttgcgcagct ccaacgcgga ggcgccgatc
     3121 aggatcagct cgcgcacctc gtccgtcact tccatcactt cgtagaggcc gatgcgcccc
     3181 ttgtagcccg tgtcgttgca cttcgtgcag ccctgtccct tgtaggtctt catcgtcttg
     3241 gcctcggcgg gcgagaagcc gacctcggcg agcgcctccg gcggaaggtt ctgctcgacc
     3301 ttgcagtccg cgcagatgcg gcggatcagg cgctgcgcct ggatgaggtt gaccgaggtg
     3361 gccacgagga aaggctcgat gcccatgttc atgaggcggg agatggtcga gggggcgtcg
     3421 ttggtgtgga gggtggaga
//
LOCUS       DBNGOH010000242         5639 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3151727, whole genome shotgun sequence.
ACCESSION   DBNGOH010000242 DBNGOH010000000
VERSION     DBNGOH010000242.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5639)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5639)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5639
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3151727"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..456)
                     /locus_tag="AC159P_04540"
     CDS             complement(<1..456)
                     /locus_tag="AC159P_04540"
                     /inference="COORDINATES: protein motif:HMM:NF037047.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694753.1"
                     /translation="MSVGNNPQPAGEDFAVAEPNESPLTILAIASFFKGNDFIRECRR
                     RGGRAVLLTREKLLGEDWARDALDDILAVPGRGGEELYLEAAACVARRRRVDRVVALE
                     EYDVLTAARLREHLGLPGMGSTVARRFQDKLAMRVRACEIGVAVPEFVHL"
     gene            482..769
                     /locus_tag="AC159P_04545"
     CDS             482..769
                     /locus_tag="AC159P_04545"
                     /inference="COORDINATES: protein motif:HMM:NF024934.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR3694754.1"
                     /translation="MASRVPVETLTLREVAQRLARLLLAEERARGERTKGVFLLTLTL
                     TNQQIAARVGSVREVVSRALVLLRQDGLIALEDRWLSILDEAALSSYAGED"
     gene            1011..2225
                     /locus_tag="AC159P_04550"
     CDS             1011..2225
                     /locus_tag="AC159P_04550"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF048461.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HIR3694755.1"
                     /translation="MLATNTLPGADRGVADRQHLIAAVRGRDPGPTLVVVCSLHGNEP
                     AGVVAARRLCAWLRRAAPLLRGEAVLLAGNTRALARGVRYVDADLNRHWTPENIARAE
                     SGGLSAETGSEDRELRELLGEFRQASSRARGTLYFLDLHTMSANGAPFATLGDTLRNR
                     RFALNFPTTVVLGLEEQLEGTMLEHVNNAGAVTLGFEAGQHEAPSSVEHHAAVIRIAL
                     VAAGLLRRDDAPEFERAHALLERAGGGTRIVEVRHRHAITPFDEFGMKPGFENFQPVR
                     RGETLAQDRRGQIGARETGLVLLPLYQKLGDDGFFLGREVRPFWLKLSAVLRRLKVGD
                     YVHLLPGVRRLAADGSSLVVNTRVARLVPLQIFHLLGFRRRRWGGGLLEVSRRRYDLA
                     GPREVGNEGGSR"
     gene            2222..3925
                     /locus_tag="AC159P_04555"
     CDS             2222..3925
                     /locus_tag="AC159P_04555"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010961088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HIR3694756.1"
                     /translation="MMAVKEDVRRAEEALEVSLTGHLLLDNPLLNKGSAFPEEERRAF
                     GLLGLLPPHPSTIEEQLVRTYENYRRKETDLERYIFLGSLQDRNETLFYRLLQAHITE
                     MMPIIYTPTVGLGCQSYSHLFRRPRGLYISYPQRGEIDAILANAPTAEPSVIVVTDGE
                     RVLGLGDLGVGGMGIPVGKLSLYTLCAGIHPRTTLPIQLDAGTNNRELLEDPLYLGWR
                     HERVRGEAYADFVEAFVEGVARKFPRALLQWEDFAKHNAARLLERYRDRLCTFNDDIQ
                     GTGAVTLAGLLAATKLSGARLSEQRITILGAGSSATGISEQIVTAMRAEGLSEQEARA
                     RLWLLDSGGLVHAGRAGLEPFKQKFARPVEQTARWGPAPDGGFALADVVRNVRPTVLI
                     GTSAQPGAFTEEVVREMARHAERPLIFPLSNPTSKSEATPRDLVAWTEGRALVATGSP
                     FPEVSYGGRTIRTGQCNNSFIFPGVGLGVIAAAARRVTDGMFVAAARSLAELSPALRC
                     FAQRIKIATRYCRPLRVDLQHLVLAVIPLGKARMNPYVWFTGDIDEGRECARIQLSDS
                     C"
     gene            complement(3952..4566)
                     /locus_tag="AC159P_04560"
     CDS             complement(3952..4566)
                     /locus_tag="AC159P_04560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR3694757.1"
                     /translation="MALIGLLALATSAAASAQSKETREHRVIPLRPMTQDVEVLYGDP
                     EAAGQPFVMRIRELPGTIIPPHKHPVDEHITVVEGTIYFAVGERFDHAAMKEINTGGY
                     AFIPAGSTMSGHIPDGAIVQVHGTGPFHIHWRAGTGWRMGHKTLDDPDAATHFKFKKG
                     ERVVAKRGRGRVRQAYDSGEILQYEIEGEDGTRFMALEGELRRG"
     gene            complement(4678..>5639)
                     /locus_tag="AC159P_04565"
     CDS             complement(4678..>5639)
                     /locus_tag="AC159P_04565"
                     /inference="COORDINATES: protein motif:HMM:NF033206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ScyD/ScyE family protein"
                     /protein_id="HIR3694758.1"
                     /translation="AGSGGAGLCEEGPEGTRCYGATGSVTSIDLRKGSITRVATGLPS
                     LASQDGSFATGPHDISLHGRGNMFVTLGYGGNPDSRTTSLGPAGANFARLARIDASGN
                     WSLGEDLGAYEAAANPTGDEVDSNPYGILALAGRQVVADAGANALNEVAANGAVSTLA
                     VFPDRMVEAPPFLELPPGTEIPMDAVPTSIAVGPDGLFYVGQLTGFPFPVGGANVYRV
                     PAQGGTPEVYAGGFTAIVDVAFGPDGSLYVLEIAKNGLLAAFIANDWTGALIRVAPDG
                     TRTEIAAGELFAPGGVAVGPDGAIYVTNNSIFSGSGQVLRIVP"
BASE COUNT          899 a         1922 c         1866 g          952 t
ORIGIN      
        1 caggtgaaca aactccggga ccgcgacgcc gatctcgcac gcccggacgc gcatcgccag
       61 cttgtcctgg aagcggcggg cgacggtgct ccccatgccc ggcaggccga ggtgctcgcg
      121 caagcgcgcc gccgtcaaca cgtcgtactc ttcaagcgca acgactcggt cgacgcgccg
      181 ccgccttgcg acgcaggccg ccgcttcgag gtaaagctcc tcgcccccac ggcccggtac
      241 ggcgaggatg tcgtcgagtg cgtcgcgcgc ccaatcttca ccaagcaact tttcccgcgt
      301 cagaagaacc gcccgcccgc cccgccgccg gcactcgcgg ataaagtcgt tgcccttgaa
      361 gaagctggcg atagccaaaa tggttagagg agattcattt ggttccgcca cggcgaaatc
      421 ctcccccgcg ggttgcgggt tattacccac actcatgccg aaaacctcat agactcagtg
      481 tgtggcgagt cgcgtgccgg tcgagacgct caccctgcgc gaggtcgcac agcgcctcgc
      541 gcgcctgctg ctggccgagg agcgcgccag aggcgagcgc accaaaggag tcttcctcct
      601 aacgcttacg ctgaccaacc agcagatcgc ggcgcgcgtc ggctcggttc gggaggtggt
      661 ctcgcgcgcc ctcgtcctgc tccggcagga cggccttatc gcgctggaag atcgctggct
      721 atcgatcctg gatgaagcgg cactctcgtc atacgccggc gaagactgag gacgcgcccg
      781 catcacgggg cgcccggcga gcgattcgtg cgggtaaagt tcctctaggg gtgggttaaa
      841 gcccgcactt aacggtatcc cgcttgagac gcgctcgcgt gtttccggga cattgtaggg
      901 aaatccctcc tcgacgttcg gtcggcatac tcgttgctaa tttttattcg ggggctctcg
      961 cccgttcatc ccagagagga gtcgccgagc gcgcggggtt cttccgactt atgttagcta
     1021 ccaacaccct tcccggcgcg gaccgaggcg ttgccgaccg tcaacacctc atcgcggcgg
     1081 tcaggggccg cgaccccggc ccgactttgg tggtcgtgtg tagcctgcac ggcaacgagc
     1141 cggcgggcgt cgtcgcggcc cgccggttgt gtgcctggtt gcggcgcgcc gcaccgctgc
     1201 tccgcgggga ggccgtgttg ctggccggga acacgcgcgc gctggcgagg ggcgtgcgct
     1261 atgtggacgc cgacctcaac cggcactgga cgcccgagaa catcgcgcgc gcggagtccg
     1321 gcggcctctc cgccgagacg gggtccgaag atcgggagct gcgcgagctg ctcggagagt
     1381 tccggcaggc ttcctcgcgt gcgcggggca cgttgtactt cctcgacctg cacacgatgt
     1441 ccgcgaacgg cgcgcccttc gccaccctgg gcgacacgct gcgcaaccgc cgcttcgcgc
     1501 tgaatttccc gacgacggtt gtgctcgggc tggaagaaca gctcgagggg acgatgctcg
     1561 aacacgtgaa caacgcgggg gccgtgacgc tcggcttcga ggccggccag cacgaggccc
     1621 cttcttcggt cgaacatcac gcggcggtga ttcggatcgc gctggtcgcg gcgggtctgc
     1681 tacgccgcga cgacgcgccg gagtttgagc gcgcgcacgc tctgctcgaa cgcgcgggcg
     1741 gcggcacgag gatcgtcgaa gtgcgccacc gccacgccat cacgcccttc gacgagttcg
     1801 gcatgaaacc gggcttcgag aactttcaac ccgtccggcg cggcgagacg ctggcgcagg
     1861 accgccgcgg ccagatcggg gcgcgcgaga cggggctggt gctgctgccg ctctaccaga
     1921 aactcggcga cgacggcttt tttctcggac gtgaggtcag gccgttctgg ctgaagctgt
     1981 cggcggtgct ccgccgcctc aaggtcggcg attacgtcca cctgctgccc ggcgtccgcc
     2041 gactggcggc ggacggcagc tcgctcgtcg tcaacacgcg cgtcgcgcgc ctcgtcccgt
     2101 tacagatttt tcacctgctc ggcttccgca ggcggcgctg gggcggcggc ctgctcgaag
     2161 tcagccggcg gcgctacgac ctggccgggc cccgcgaagt cgggaacgaa ggaggctcaa
     2221 gatgatggcc gtcaaggaag acgtgaggcg ggcggaagaa gcgctggagg tttcgctgac
     2281 gggccacctg ttgctggata acccgctgct caacaagggc agcgccttcc cggaagagga
     2341 gcggcgcgcg ttcgggctgc tgggccttct gccgccgcac ccttcgacca tcgaagaaca
     2401 actggtccgc acctacgaga actaccggcg gaaggagacc gacctcgagc gctacatctt
     2461 tctcggctcg ctgcaagacc gtaacgagac gctcttctac cgcctgctgc aagcccacat
     2521 cacggagatg atgccaatca tctacacgcc gaccgtcggg ctgggctgcc agagctacag
     2581 ccacctcttc cgccgcccgc gcggcctcta catctcttac ccgcagcgcg gcgagatcga
     2641 cgccatcctc gccaacgcgc cgaccgcaga gccgtcggtc atcgtcgtca ccgacggcga
     2701 gcgcgtcctc ggcctcggcg acctcggcgt cggcggcatg ggcatccccg tcggcaagct
     2761 ctcgctctac acgctctgcg cgggcatcca cccgcgcacg accctgccca tccagctcga
     2821 cgcgggcacg aacaaccgtg agctgctcga agacccgctc tacctcggct ggcggcacga
     2881 gcgcgtgcgc ggcgaggcgt acgccgattt cgtcgaggcg ttcgtcgagg gcgtggcgcg
     2941 caagttcccg cgcgccctgc tgcaatggga ggacttcgcc aagcacaacg ccgcgcggct
     3001 gctcgaacgg tatcgtgacc gcctgtgcac cttcaacgac gacatccagg gcaccggcgc
     3061 ggtgacgctc gccgggctgc tggccgcgac gaagctctcg ggcgcgcggc tctccgaaca
     3121 gcgcatcacc atcctcggcg cgggctcttc ggccacgggc atcagcgaac agatcgtgac
     3181 ggcgatgcgc gccgaaggtc tgtcggagca ggaggcgcgc gcgcggctgt ggctgctgga
     3241 cagcggcggg ctggtgcacg cggggcgcgc gggccttgag cctttcaaac aaaaattcgc
     3301 gcgacccgtg gagcagacgg cccggtgggg gccggccccg gacggcggct ttgctctcgc
     3361 ggacgtggtc aggaacgtgc gcccgaccgt cctcatcggc acgtctgcac agccgggggc
     3421 gttcaccgaa gaggtcgtgc gcgagatggc acgacacgcc gagcggccac tcatcttccc
     3481 tctctccaac ccgacctcga agagcgaggc gacgccgcgc gacctcgtcg cgtggacgga
     3541 agggcgcgcg ctggtcgcca ccggcagccc gttcccggag gtcagctacg gcgggcggac
     3601 gatccgcacg gggcagtgca acaactcctt catcttcccc ggcgtcggcc tcggcgtgat
     3661 cgccgcggct gcacgccgcg tgaccgacgg gatgttcgtc gccgccgcgc gctctctcgc
     3721 ggagctgtcg cccgcgctgc gctgctttgc ccaacgaatc aaaattgcca ctcgatattg
     3781 tcggcctttg agagtagacc tccaacatct tgtgttggca gtcatcccgt tgggcaaagc
     3841 acggatgaac ccttatgtgt ggttcaccgg cgacattgat gagggccgtg aatgcgcccg
     3901 catacaactc tcagatagct gttgacccgc cggtccggtt accccgccgc ttcagccacg
     3961 ccgcaactct ccctctaacg ccatgaaacg cgtcccgtct tcgccttcga tttcgtactg
     4021 gaggatttcg ccggaatcgt aagcctggcg gacgcggccc ctgccccgct tggcgacgac
     4081 gcgctcaccc ttcttgaatt tgaaatgggt cgcggcgtca gggtcgtcga gcgtcttgtg
     4141 tcccatgcgc cagccggttc cggcgcgcca gtggatgtgg aacggccccg tgccgtgaac
     4201 ctggacgatg gcgccgtcgg ggatgtggcc ggacatcgtg ctcccggcgg ggatgaaggc
     4261 gtagccgccg gtgttgattt ccttcatcgc cgcgtggtcg aacctctcgc ccacggcgaa
     4321 gtagatcgtg ccctcgacca ccgtgatgtg ctcgtcaacg gggtgcttgt gcggcgggat
     4381 gatcgtgccg ggcagctcgc ggattctcat cacgaacggt tggcccgccg cctccgggtc
     4441 gccgtagagg acttcgacgt cctgggtcat cggcctgagg gggatgacgc ggtgctcgcg
     4501 cgtctctttc gactgtgcgg acgcggcggc agaagtggct agagccagca ggccaatcag
     4561 cgccattatg aacacgcttt tcgtcaacat tcgggtgcct ccgattgtgg aagtctcaaa
     4621 ctcagtcggg ggttactatc tgccgcctgt ttatccggct atcgagaggg aaatccttca
     4681 ggggacaatc ctgagcacct gacctgatcc gctgaagatg ctgttgtttg ttacgtagat
     4741 tgcgccgtcg gggccgacgg ccacgcctcc cggcgcgaag agttcgcccg ccgcgatctc
     4801 cgtccgcgtg ccgtcgggcg cgacgcggat gagcgcgccc gtccagtcgt ttgcgatgaa
     4861 agcggcgagc agtccgtttt tcgcgatctc cagaacgtag agcgaaccgt cggggccgaa
     4921 ggctacgtca acaatcgcgg tgaacccgcc ggcgtagacc tccggcgtcc cgccctgcgc
     4981 cggcacgcgg tagacgttcg ccccgccgac ggggaacggg aagcccgtga gttggccgac
     5041 gtagaaaagc ccgtcaggcc cgacggcgat tgaagtcggc acggcgtcca tcggaatctc
     5101 cgtcccgggc ggaagctcaa ggaacggcgg ggcctcaacc atccggtcgg ggaagacggc
     5161 cagcgtagag accgctccgt tcgccgccac ttcgttgagt gcgttagcgc cggcatcggc
     5221 cacgacctgc ctgccggcca gtgcgaggat gccgtaaggg ttcgaatcca cttcatcacc
     5281 tgtcggattc gccgcggcct cgtaagcgcc gaggtcttcc ccgagactcc agttcccact
     5341 cgcgtcgatg cgggcgaggc gggcgaagtt cgcgcccgcc gggccgagag aagtcgttcg
     5401 cgagtcaggg ttcccgccgt agccgagggt cacgaacata ttgccgcgcc cgtggagcga
     5461 aatgtcatgc ggacctgtgg cgaaactgcc gtcctggcta gcgagggacg gcaagcctgt
     5521 ggcgacgcgc gtgatacttc ctttacgcag gtcgatgctc gtgaccgagc cggtcgcccc
     5581 gtagcaacgt gtgccttcgg gcccttcctc gcaaaggccg gcgccgccgc tgcccgcct
//
LOCUS       DBNGOH010000243         2369 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3151833, whole genome shotgun sequence.
ACCESSION   DBNGOH010000243 DBNGOH010000000
VERSION     DBNGOH010000243.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2369)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2369)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2369
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3151833"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1480
                     /locus_tag="AC159P_04570"
     CDS             <1..1480
                     /locus_tag="AC159P_04570"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HIR3694759.1"
                     /translation="RGRIVRQLLTESLLLSLVGGGAGLVLAYWLTDMMTAFFPVIPYT
                     IALDMAPDARALVYALAVSLLTGVIFGLAPALQASNPDVVTELKGGTRAAGHRRRRLT
                     LRDALVVSQIALSLLVLVCAGLFVKSFRNAKAINPGFATDNVLLAHVNPGLFGYEKEP
                     GRDFYRRLLERVRQLPGVESAGAAYWLPLGDSSSGWGPVTRADQPAPPPGEGQGAFAN
                     MVAPGYFDAVGMPLLKGRDFDVRDREGVAHEAVVINETLAARLWPGEDPTGKLLRVGR
                     TDPSVLEVVGVVKDAKYRTLGEPPREHLFVSIDQTYRPGLFLVIRTKGNPAAYVGGVR
                     EAVRDLDPRMPLYAVKTMGEHLTWAWWAPNMAASLSTAFGLLALALAATGLYSVIAYS
                     VSRRTREIGIRMALGAQPRDVLRVVAGQGIRLLLVGMGAGFAAALAATRVLSSLLFGV
                     SATDPAVLASIALLLAAVALLAGYVPARRATKVDPMVALRYE"
     gene            1614..>2369
                     /locus_tag="AC159P_04575"
     CDS             1614..>2369
                     /locus_tag="AC159P_04575"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3694760.1"
                     /translation="MMETIWNDVRYGLRTLAKRPAFSSVAVLTLALGIGANTAIFSVI
                     NGVLLRPLPYAEPERLVSVWFTSTGEREESFASFPDLADWRAGTSSFEELAAYRNSPV
                     TLNGFGDPQRVEGVSVSAGFFPLLGVTPIAGRTSRPEEDKPGGEQVAVISYGFWQRNF
                     GDDRSAVGRRLHLDGESHTIVGILPRGFRPPLFTEDKEIYTTVAGEGGNLTSRGARVV
                     RVIARLKPGVTLEQAQADIEAVAGRLAQQYPESN"
BASE COUNT          333 a          838 c          810 g          388 t
ORIGIN      
        1 gcgcgggcgc atcgtgcgtc aactgctgac ggaaagcctg ctgctctcgc tcgtcggcgg
       61 gggggcgggc cttgtgctag cctactggct gacggacatg atgacggcat tctttcccgt
      121 catcccctac accatcgcgc tcgacatggc gcccgacgcg cgcgcgctcg tctacgcgct
      181 cgccgtctca ctgctcacgg gggtgatatt cggtctggcg ccggccctcc aggcgtcgaa
      241 cccggatgtc gtcaccgagc tgaagggcgg cacccgtgcc gccggtcaca ggcggcggcg
      301 cctcacgctg cgcgacgcgc tcgtcgtctc gcagatcgcg ctctcgctcc tcgtgctggt
      361 ctgcgccgga ctcttcgtca agagcttccg gaacgccaag gcgatcaacc ccggcttcgc
      421 caccgacaat gtcctgctcg cccacgtcaa ccctggcctc ttcggctacg agaaggagcc
      481 gggccgtgac ttctaccgcc ggctgcttga gcgcgtccgg cagttgcccg gcgtcgagtc
      541 ggcgggcgcg gcgtactggc tcccgctcgg cgacagctcc agcggttggg ggccggtcac
      601 ccgcgccgac cagccggcgc cgccgcccgg cgaggggcag ggtgcgttcg ccaacatggt
      661 cgcgcccgga tacttcgacg cggtcgggat gccgctcctg aaggggcgcg acttcgacgt
      721 gcgcgaccgc gaaggagtcg cgcacgaggc cgttgtcatc aacgagacgc tggccgcgcg
      781 gctctggccc ggcgaagacc ccacggggaa attactgcgc gtcggacgca cagacccttc
      841 ggtgctcgaa gtcgtcggcg tcgtgaagga cgcgaagtac cgcacgctcg gcgagccgcc
      901 gcgcgaacac cttttcgtct ccatcgacca gacctaccgc cccggcctgt tcctcgtcat
      961 ccgcacgaag ggcaacccgg cggcctacgt cggcggcgtg cgcgaggccg tgcgcgacct
     1021 cgacccgcgg atgcccctct acgcagtcaa gacgatgggc gagcacctga cctgggcctg
     1081 gtgggcgccg aacatggccg cgtcgctttc gaccgccttc gggctgctgg cgctggcgct
     1141 ggcggcgacg ggcctctaca gcgtgatcgc ctactcggtc agccggcgca cgcgcgagat
     1201 cggcatccgc atggccctcg gcgcgcagcc gcgcgacgtg ctgcgcgtgg tcgccgggca
     1261 ggggatcagg ctcctgctcg tcgggatggg ggcgggcttc gccgccgcgc tcgccgcgac
     1321 gcgcgtgctg tcgagcctgc tcttcggagt ctcggcgacc gacccggcgg tcttggcctc
     1381 catcgcgctg ctgctcgccg ccgtcgcgct gctcgccggc tacgtgcccg cgcgccgtgc
     1441 gacgaaggtc gacccgatgg tcgcacttcg ttacgaataa agccctcaca ggtggcaggc
     1501 gaagagtgac ggcaaagggc cgtgaccggt taaccgcagt gcgggacgag tgagagcctg
     1561 tctttcactc gtcacctgtc acctgtcatc tgtcacggct ttagggggtg tgaatgatgg
     1621 agactatctg gaatgacgtg cgctacggac tgcggacgct ggcgaagcgc cccgcgttct
     1681 cttcggtcgc ggtcttgacg ctggcgctcg gcatcggcgc gaacacggcc atcttcagcg
     1741 tcatcaacgg cgtgctgctc cgcccgctcc cctacgccga gccggagcgc ctcgtctccg
     1801 tctggttcac ttcgacgggc gagcgtgaag agagcttcgc ctcctttccc gatctggccg
     1861 actggcgcgc agggacaagc tctttcgagg agctggcggc ctacagaaac tcccccgtca
     1921 cgctgaacgg cttcggcgac ccgcagcggg tcgagggggt gtcggtgtcg gcagggttct
     1981 tcccgctcct cggcgtgacg ccgatagccg gtcgcacttc ccgccccgaa gaggacaagc
     2041 ccggcggcga gcaggtcgcc gtcatcagtt acggattctg gcagcgcaac ttcggcgacg
     2101 accgcagcgc ggtggggcgg cggctccacc tcgacggcga gtcgcacacc atcgttggca
     2161 tcctgccgcg cggcttccgc ccgccgctct tcaccgaaga taaagagatt tacacgaccg
     2221 tcgcgggcga aggcggcaac ctcacttcgc gcggggcgcg cgtggtccgc gtcatcgcga
     2281 ggctcaagcc gggggtgacg ctggagcagg cgcaggccga catcgaggcg gtcgcggggc
     2341 gcctcgcgca gcaatatccg gagtcgaac
//
LOCUS       DBNGOH010000244         4868 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1136861, whole genome shotgun sequence.
ACCESSION   DBNGOH010000244 DBNGOH010000000
VERSION     DBNGOH010000244.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4868)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4868)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4868
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1136861"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..347)
                     /locus_tag="AC159P_04580"
     CDS             complement(<1..347)
                     /locus_tag="AC159P_04580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694761.1"
                     /translation="MSSNPLARAAPPTALLWLFVAVTQLVTGLYVGGDIDPPPAYVLL
                     YPLAFLWVIGWWMRRDCRARGVAWVLDMGLFLYMAWPFVMPYYLFKTRGVRALLAVGA
                     FVAVYLLAFLAGV"
     gene            complement(391..1569)
                     /locus_tag="AC159P_04585"
     CDS             complement(391..1569)
                     /locus_tag="AC159P_04585"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HIR3694762.1"
                     /translation="MRSPPARHESNATPHAPSASWPTVADAGEPRPLAAARWDWSAVR
                     RVLVVRLRSIGDTVLATPSLRALRRYLPQARVDILLEDWVAPLVEGSADVDRVVTVKR
                     RSNRSRFEAARELRAAEYDVAFNLHGGSTAALLMRASGAKHRVGYRDYAYASLHNHLA
                     PPSAELWGTEKTHSAEQQLALLGYAGVPVTDRPATRLFVNERASEVVSAKLRGAGLEE
                     GEPFALMHPAAAFDSKTWAAENFARVADHLAAPGLRVVAVASPEESGVIDEVRRLARR
                     TPPIGFTDLLLPEVAALASRAVLFVGNDSGVAHIAAAFAVPSVVVFGSSNVAHWRPWT
                     TAPSEVVREEMPCAPCPGYTCAEFGEAQCIRRVTVERVVEAVGRVTAAAGLAFDEARA
                     "
     gene            complement(1544..1735)
                     /locus_tag="AC159P_04590"
     CDS             complement(1544..1735)
                     /locus_tag="AC159P_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HIR3694763.1"
                     /translation="MSIEPDLLEILVCPACRGRVELKADGSALKCVECRRVYPVRDGI
                     PAMVVEEATVEDEKPAGSA"
     gene            complement(1812..3173)
                     /locus_tag="AC159P_04595"
     CDS             complement(1812..3173)
                     /locus_tag="AC159P_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002637216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HIR3694764.1"
                     /translation="MVAALHPPGPKTLMPGGQMRAFRRDPLGFLTRLARRYGDVAQFR
                     IGPQKAFLLSHPDDIRDVLVTHHERFHKGRALQRSKRLLGEGLLTSEGAHHRRQRRIA
                     QPAFHRQRVASYGAVMVEYAARAAERWREGRTLDISEEMMRLTLAIAGKTLFDADVEQ
                     HDADEVGAALTEVMELFGYLMLPYSELLEKLPFPLPPVVRFQRARKRLDAVIYRIIDE
                     RRRSGADRGDLLSMLLLAEDEEDAGARMSNEQVRDEVMTLFLAGHETTANLLAWTWYL
                     LSQHPEVEARLHAELDAVLAGGRLPTVEDIPALRYTEMVVAETMRLYPPAWTVGRLAI
                     EDHEVGGYLIPKGSLVLLSQYVVHRDPRFFPDPERFDPERFRPEAKEARPPFAYFPFG
                     GGVRRCIGEGFAWMEATLVVATLARRWRMRLAPGHTVRPAPRITLRPSKDGVVMTLNS
                     REP"
     gene            3267..4028
                     /locus_tag="AC159P_04600"
     CDS             3267..4028
                     /locus_tag="AC159P_04600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694765.1"
                     /translation="MSLEFAIEYPCALRARYKEEHLRAWGRLGSLHTRLTTGEASAPS
                     DEKVRFVERAADELEKMREETAVCLKCPAHLPTETAGEGEAVGCLGRITYPVEAQFEK
                     FLADRVQLALDTMDVPDQPRLLRVLLDSDSPFDGEGTKHLRSVTTVSGLRFFELRLPI
                     RLAREAAHLTTDNLFDALAGFTSDDAERTTYTRELPLAATPVYYDFLDLILRHDLSDS
                     ERDRARARSRNYQQFLRLLAALELAESLRARVLLD"
     gene            complement(4094..>4868)
                     /locus_tag="AC159P_04605"
     CDS             complement(4094..>4868)
                     /locus_tag="AC159P_04605"
                     /inference="COORDINATES: protein motif:HMM:NF025690.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HIR3694766.1"
                     /translation="VGIQRRASGPRAMGEAEKHKSLDETVEAALLDADLFVKYKAPHR
                     AVRRLQEAIAHYPRSIVLREKLREVAVSYQQPDEAARQCLALVTLYISRENFEAAHER
                     LLEAKHIDPRINIAPGFEAIRRARRPDLQHAQAAAAPPADPRKAQVTFAGDLSTVSVF
                     DAIQVIENSRLTGALALDNQERRGRVLFNEGRIVGAECAGREALEAFRLVVEFTAGSF
                     DFEKSGDEYPITINAPSNTNLILDSLRQMDEENEEGREP"
BASE COUNT          761 a         1684 c         1684 g          739 t
ORIGIN      
        1 atgactcccg cgagaaacgc cagcaggtag acggccacga acgcgccgac cgcgaggagc
       61 gccctcacgc ccctcgtctt gaagagataa taaggcatga cgaacggcca tgccatgtaa
      121 aggaacagcc ccatgtcgag cacccaagcg acgccgcgcg cgcggcaatc cctccgcatc
      181 caccagccga tgacccacag gaacgccagc gggtagagca gcacgtaagc cgggggcggg
      241 tcgatgtcgc ccccgacgta gaggccggtg acgagctgcg tcacggccac gaacagccag
      301 agcagggcgg tcggcggcgc ggcgcgtgcg agcgggttgg aactcatgcg cgtgttctcc
      361 acggtcggcg gcgaaggggc ggtctcgcct tcaggcgcgc gcctcgtcga aggcgaggcc
      421 tgccgccgcc gtcacacgcc cgacggcttc gacgacccgc tcgacggtga cgcggcggat
      481 gcactgggct tcgccgaact ccgcgcaggt gtagccgggg cacggggcgc agggcatctc
      541 ttcgcggacg acctccgaag gcgcggtcgt ccacggtcgc cagtgggcga cgttcgagga
      601 gccgaagacg acgaccgaag ggactgcgaa cgcggcggcg atgtgcgcca cgccgctgtc
      661 gttgccgacg aagagcacgg cgcgcgaggc gagcgccgcc acctccggaa gcagcaggtc
      721 cgtgaagccg ataggcggcg tccggcgcgc gaggcggcgc acttcgtcga tgacgccgga
      781 ctcttcgggc gacgcgacgg cgacgacgcg caagcccggc gcggcgaggt ggtctgcgac
      841 gcgcgcgaag ttttcggcgg cccacgtctt cgagtcgaag gccgccgccg ggtgcatcag
      901 cgcgaacggt tcgccctctt cgagacccgc gccgcgcagc ttcgccgaga cgacttcgga
      961 cgcgcgctcg ttgacgaaga gtcgcgtggc ggggcggtct gtgacgggca cgcccgcata
     1021 gcccaggagc gcgagctgct gctccgcgga gtgcgtcttc tccgtccccc acagctccgc
     1081 ggaaggcggc gcgaggtggt tgtgcagaga ggcgtaggcg tagtcgcggt agccgacgcg
     1141 gtgcttcgcg cccgaggcgc gcatgagcag cgcggcggtg ctcccgccgt ggaggttgaa
     1201 ggccacgtcg tattcggcgg cgcgcagctc gcgcgcggcc tcgaagcgcg agcggttgct
     1261 ccggcgcttc accgtgacga cgcggtcaac gtccgcagag ccttcgacga gcggcgcgac
     1321 ccagtcttcg agcaggatgt ccacgcgcgc ctgcggaagg taccggcgca gcgcgcgcag
     1381 cgaaggcgtc gccagcaccg tgtcgcctat cgagcgcagc cgcacgacga gcacgcggcg
     1441 caccgccgac caatcccacc gcgccgccgc cagcgggcgc ggctctcccg cgtccgcgac
     1501 cgtcggccaa gacgccgagg gcgcgtgagg ggtcgcgttc gactcatgcc gagccggcgg
     1561 gcttctcatc ttcaaccgtc gcctcttcga cgaccatcgc cgggatgccg tcgcgcacag
     1621 ggtagacgcg acggcactcc acgcacttca acgcgctccc gtccgccttc aactccaccc
     1681 tccccctgca cgcggggcag acgagtatct ccaacaggtc tggttcgatg ctcaattcga
     1741 ttctccttaa agacgtaaac cgtgaaccgt aaaccgtgat aagtgaaaaa ccggttctca
     1801 cctgtcacgg ttcacggttc acggctgttg agtgtcatca ccacgccatc tttgctcggt
     1861 cgcagggtga tgcgcggcgc gggccggacg gtgtggccgg gggcgaggcg catgcgccag
     1921 cggcgcgcga gcgtggcgac gacgagcgtg gcctccatcc aggcgaagcc ctcgccgatg
     1981 cagcggcgca cgccgccgcc gaaggggaag taggcgaacg gcggtcgcgc ctctttcgct
     2041 tccgggcgga agcgctcggg gtcgaagcgt tcggggtccg ggaagaagcg cgggtcgcga
     2101 tggacgacgt attgactcag caggacgagc gaccctttcg ggatcaggta gccgccgact
     2161 tcgtggtctt cgattgccag ccgcccgacc gtccaagcgg gcgggtacag gcgcatcgtc
     2221 tcggccacga ccatctccgt gtagcgcaag gcggggatgt cttcgaccgt gggcagccgc
     2281 ccgcccgcga gcaccgcgtc gagttctgcg tgcagccgcg cctcgacctc cgggtgctgc
     2341 gagagcagat accaagtcca cgcgagcagg ttggccgtcg tctcgtggcc cgcgaggaag
     2401 agcgtcatca cctcgtcgcg cacctgctcg ttcgacatgc gcgcgcccgc gtcctcttcg
     2461 tcctcggcga gcagcagcat cgagagcagg tcgccgcgat ccgcgccgct ccggcgccgc
     2521 tcgtcgatga tgcggtagat gacggcgtcg agcctcttgc gggcgcgctg gaagcggacg
     2581 accggcggga gcgggaacgg cagtttctcc agcagctccg agtaaggcag catcaggtag
     2641 ccgaacagct ccatgacctc ggtgagcgcc gcgcccacct cgtccgcgtc gtgctgctcc
     2701 acgtcggcgt cgaacagggt cttgccggcg atggcgagcg tcaggcgcat catctcttcg
     2761 gagatgtcca gtgttcggcc ctcgcgccaa cgctccgccg cgcgcgccgc gtactcgacc
     2821 atcaccgcgc cgtacgaggc gacgcgctgg cggtgaaacg caggctgcgc gatccgccgc
     2881 tgccgccggt ggtgcgcgcc ctcgctcgtc agcaagcctt cgcccaaaag gcgcttcgag
     2941 cgttgcagcg cgcgcccctt gtggaagcgc tcgtggtgcg tgacgagcac gtcgcggatg
     3001 tcgtccgggt ggctcagcag gaacgccttt tgcggcccga tgcggaactg cgccacgtcg
     3061 ccgtaccggc gcgcgagccg cgtcaggaag ccgagcgggt cgcgccggaa agcgcgcatc
     3121 tggccgcccg gcatcaatgt tttcggcccc ggagggtgta gtgcggctac catctttctt
     3181 ctgctattat agcccgcgac ccgaagccgc gcgcaaaagc cgcgacgctt tttcgggtgt
     3241 cacaaacgag acctcaagag ggacgcttgt ctttagaatt cgccatcgag tacccctgcg
     3301 cgctgcgcgc gcgctacaag gaggagcacc tgcgcgcgtg gggccgtctg gggagtctgc
     3361 acacgcgcct gacgaccggc gaggcttcgg ccccttcgga cgagaaggtg cgcttcgtcg
     3421 agcgcgccgc cgacgagctg gagaagatgc gcgaggagac ggcggtctgc ctgaaatgcc
     3481 ccgcgcacct gccgaccgag acggcgggcg agggcgaggc cgtcggctgc ctcgggcgca
     3541 tcacctaccc cgtcgaggcg cagttcgaga agttcctcgc cgaccgcgtc cagctcgcct
     3601 tggacacgat ggacgtgccg gatcagccgc gcctcctgcg cgtcctgctc gacagcgact
     3661 cgcccttcga cggcgagggc accaagcacc tgcggagcgt gaccacggtc agcggcctgc
     3721 gcttcttcga gctgcgcctg ccgatccgcc tcgcgcgcga ggccgcgcac ctcacgaccg
     3781 acaacctctt cgacgccctc gccggcttca cctccgacga cgccgagcgc acgacctaca
     3841 cccgcgagct gccgctcgcc gccacgcccg tctactacga ctttctcgac ctcatcctcc
     3901 gccacgacct ctcggactcc gagcgggacc gcgcgcgcgc gcgcagccgc aactaccaac
     3961 agttcctccg cctcctcgcc gccctagaac tcgccgagtc gctccgcgcc cgagtcctgc
     4021 tagactgaca aaaagccgtg aaccgtaaac cgtgaaccgt gataagtaag acccggtttt
     4081 tactcatcac ggtttacggt tcacggcctt cttcgttctc ttcgtccatc tgccggagcg
     4141 agtcgaggat gaggttggtg ttgctggggg cgttgatggt gatggggtac tcgtcgcccg
     4201 acttctcgaa gtcgaaggag ccggcggtga attcgacgac gaggcgaaag gcttcgagcg
     4261 cctcgcgccc cgcgcactcg gcgccgacga tccggccctc gttgaagagg acgcgcccgc
     4321 gccgctcctg attgtcgagc gcgagggcgc ccgtgaggcg cgagttctcg atgacctgta
     4381 tggcgtcgaa gacgctgacg gtggagaggt cgccggcgaa cgtgacttgg gctttgcgcg
     4441 ggtcggcggg cggcgcggcg gcggcttgcg cgtgctgaag gtctggccgc cgggcgcggc
     4501 ggatggcctc gaagccgggc gcgatgttga tgcgcgggtc gatgtgcttg gcttcgagca
     4561 ggcgctcgtg cgcggcctcg aagttctcgc gcgagatgta gagcgtgacg agcgcgaggc
     4621 actggcgcgc ggcctcgtcg ggctgctggt aggagacggc cacctcgcgc agcttctcgc
     4681 gcaggacgat ggagcgcggg tagtgcgcga tggcctcttg caggcggcgc acggcgcggt
     4741 gcggcgcctt gtacttgacg aacagatcgg cgtcgagcag cgccgcctcg accgtctcgt
     4801 ccaagctctt gtgcttctct gcctcgccca tcgccctcgg ccccgacgct cttctttgaa
     4861 taccgacg
//
LOCUS       DBNGOH010000245         3354 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3702416, whole genome shotgun sequence.
ACCESSION   DBNGOH010000245 DBNGOH010000000
VERSION     DBNGOH010000245.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3354)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3354)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3354
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3702416"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..962)
                     /locus_tag="AC159P_04610"
     CDS             complement(<1..962)
                     /locus_tag="AC159P_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M12 family metallopeptidase"
                     /protein_id="HIR3694767.1"
                     /translation="MAKRRLKVCHDRVLPEDMFRQQPLARPRSRDVFRAVLEFRKMWI
                     NGSTLRVRFMGGTAAQRASVREQAQRWAEHANLTFEFSDAPDAEIRITFDPDDGAWSY
                     IGTDARKIPQNQPTMNIGFEDGGTAAHEFGHAIGLGHEHQNPSGGIEWNEAIVIRDLA
                     GPPNNWTPEQTRHNVLEKYKADQIRGTAFDGDSIMLYFFPGKWTKSGKGTKANDDLSQ
                     TDKAFIAGTEGYPRAAIDATKLTVNAKTATSADIGKPGEEDLFTFTVKTGGRHTIATS
                     GNTDVVMKLFGPGSKTSLIAEDDDGGTGTNARIVADLIPGQYFV"
     gene            complement(1312..2499)
                     /locus_tag="AC159P_04615"
     CDS             complement(1312..2499)
                     /locus_tag="AC159P_04615"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014812752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HIR3694768.1"
                     /translation="MKNVYLAGAVRTPIGRFGGALAGLTPADLGVVVARESLKRAGLE
                     AGDVQEVVWGCARQAGAGPNVARQIAYRAGVPETVPASTVNQACGSGLKAIILGAQEV
                     LLGRARAVLAGGVESMSRVPFFAEGVRWGLRMGDAPLVDGMYRDGFQDPLSGLVMGET
                     AENLARRYEITREEQDEYALRTQQRAAAAVEAGRFDAETVAVEVKGRKGEPVAFARDE
                     HPRAETSIESLRKLAPVFSEGGTVTAGNSSGITDGAAALVVLGEEALRESGGRVEPLA
                     RIVDYEFAGVAPEVMGIGPVPAIRRLLERQKLRLDEIDLVELNEAFAAQVIACDRELG
                     FDAARLNVNGGAIALGHPIGCTGVRITTTLLHEMKRRGARRGLSTLCVSGGLGIALLV
                     ERV"
     gene            2752..>3354
                     /locus_tag="AC159P_04620"
     CDS             2752..>3354
                     /locus_tag="AC159P_04620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694769.1"
                     /translation="MNRLTTHTEIEDARRRIASLLEARAEWLCTERRGGARGTGLRRG
                     EWELSASPRGLLFSFWGTARAGAWRVLAWESDGEKISLEAARRMGAERATLELVPRAT
                     VAAGKALVSDARRAECERLATLIREALPGAKIKSAALSAGARRGEPGRYARIRLAHEG
                     AHVFATGPVVQLGAEEADAFLSSALLWFVRAAERRRDAHAH"
BASE COUNT          500 a         1137 c         1157 g          560 t
ORIGIN      
        1 tggacgaagt attggccggg gatgaggtcg gcgacgatgc gggcgttggt gccggtgccg
       61 ccgtcgtcgt cttcggcgat gagggaggtc ttcgagccgg ggccgaagag cttcatcacc
      121 acgtcggtgt tgccgctggt ggcgatggtg tggcgcccgc cggtcttgac ggtgaaggtg
      181 aagaggtctt cctcgccggg cttgccgatg tcggcggagg tggccgtctt ggcgttgacg
      241 gtgagcttcg tggcgtcgat ggcggcgcgc gggtagccct ccgtgccggc gatgaaggcc
      301 ttgtccgtct gcgagaggtc gtcgttggcc ttcgtcccct tgccgctctt cgtccacttg
      361 ccggggaaga agtagagcat gatcgagtcg ccgtcgaacg ccgtcccgcg aatctggtcg
      421 gccttgtact tctccagcac gttgtggcgg gtctgctcgg gcgtccagtt gttgggcggg
      481 ccggcgaggt cgcggatgac gatggcctcg ttccactcga tgccgcccga ggggttctgg
      541 tgctcgtggc cgaggccgat ggcgtggccg aactcgtgcg cggcggtgcc gccgtcctcg
      601 aagccgatgt tcatcgtcgg ctggttctgc ggaatcttcc gggcgtcggt gccgatgtac
      661 gaccacgctc cgtcgtccgg gtcgaacgtg atacggatct cggcgtcggg cgcgtccgag
      721 aactcgaagg tgaggttggc gtgctccgcc cagcgctgcg cctgctcgcg gacggaggcg
      781 cgctgcgcgg cggtgccgcc catgaagcgc acgcgcaagg tcgagccgtt gatccacatc
      841 ttgcggaact ccagtacggc gcggaacacg tcgcggctgc gcgggcgggc gagcggctgc
      901 tgccggaaca tgtcctcggg cagcacgcgg tcgtgacaaa ctttcaaacg tctcttggcc
      961 atctgttact cctcggtgaa acgaagtcgt gcggtcggcg gcagggccgg aaccccgccc
     1021 tcggcatgaa gcgtttcact gtcaaattag tgaagacgat tgggtcaacc gcgaggccgg
     1081 cggattgtat cactacgaag attaatggca aggaatttct gaagaagtag gcagtaggcg
     1141 gtaggcggta ggcagtcgaa tacagaagaa gcaagaagct tgagagtgag catctcaagc
     1201 ctcttgcttc attgctcgaa gagcgaagac cctttggact ttacctctca agcctcttca
     1261 ctctgcttgc tttaactgcc tgctgcctac cgcctactgc ctactctcct ctcacacgcg
     1321 ctcgaccagc agcgcgatgc cgaggccgcc gctgacgcag agcgtcgaga ggccgcgccg
     1381 cgcgccgcgc cgcttcatct cgtggagcag cgtcgtcgtg atgcgcacgc cggtgcagcc
     1441 gatggggtgg ccgagcgcga tggcgccgcc gttgacgttg aggcgcgcgg cgtcgaaccc
     1501 cagctcgcgg tcgcacgcga tgacctgcgc ggcgaaggct tcgttcagct cgacgaggtc
     1561 gatctcgtcg agccgcagct tctgtcgttc gagcaggcgg cggatcgccg ggacggggcc
     1621 gatgcccatc acctccggcg cgacgcccgc gaactcgtaa tcgacgatgc gcgccagagg
     1681 ctccacgcgc ccgcccgact cgcgcagggc ttcctcgccg aggacgacca gcgccgccgc
     1741 gccgtccgtg atgcccgaag agttgcccgc cgtcaccgtg ccgccttccg agaagacggg
     1801 cgcgagtttc cgcagcgact cgatgctcgt ctcggcgcgc gggtgctcgt cgcgcgcgaa
     1861 ggcgaccggc tcgcccttcc gccccttgac ctcgacggcg acggtctcgg cgtcgaagcg
     1921 ccccgcctcg acggcggcgg cggcccgctg ctgcgtgcgg agcgcgtact cgtcctgctc
     1981 ctcgcgcgtg atctcgtagc ggcgcgcgag gttctccgcc gtctcgccca tgacgagacc
     2041 ggagagcggg tcttgaaagc cgtcgcggta catcccgtcc acgagcggcg cgtcgcccat
     2101 ccgcaagccc cagcgcacgc cttcggcgaa gaacggcacg cgcgacatgc tctccacgcc
     2161 gcccgccagc accgcccgcg cgcgcccgag caagacctcc tgcgcgccga ggatgatcgc
     2221 cttcagcccc gacccgcacg cctgattgac ggtcgaggcc ggcaccgtct cgggcacgcc
     2281 cgcacggtag gcgatctgcc gcgcgacgtt cggccccgcg cccgcctgcc gcgcgcaacc
     2341 ccacacgacc tcctgcacgt cgcccgcttc aagcccggcg cgcttcaagc tctctctcgc
     2401 gacgaccacg ccgaggtctg cgggcgtcag ccccgcgagt gcgccgccga agcgaccgat
     2461 gggcgtccgc accgcgcccg ccagataaac gttcttcatc gaatgattcc tcgccgataa
     2521 aaagataggt gccggaagat ggaagacgac tgcgttttat tccgactcat ggatttgatt
     2581 cgatgttaac atacgccctc gcggtgcaac gaacgcgcgc ccgccctcac gcgttcggca
     2641 acaaccctat aactcgaagc caggacacct gagccgggac gagtgatgag agagtttttc
     2701 cacttgtcac ggttcacgat tcacggttca cggctttcga aggctttcgt catgaaccgc
     2761 ctcacgaccc acacggagat cgaagacgcg cggcggcgca tcgcgtcgct gctcgaagcg
     2821 cgcgccgaat ggctctgcac ggagcggcgc ggcggcgcga ggggcacggg gctgcggcgc
     2881 ggcgagtggg aactcagcgc ctcgccgcgc ggcctgctct tctctttctg gggcacggcg
     2941 cgcgcgggcg cgtggcgcgt cttggcgtgg gagtcggacg gcgagaaaat ctcgctcgaa
     3001 gccgcgcgcc gcatgggcgc cgagcgggcg acgctcgaac tcgtgccccg cgcgacggtc
     3061 gcggcgggga aggcgctggt ctcggacgcg cgacgcgcgg agtgcgagcg gctggcaacg
     3121 ctcatccgcg aggcgctgcc cggcgcgaag ataaagagcg cggccttgag cgcgggcgcc
     3181 cgccgcggcg agccggggcg ctacgcgcgc atccgtctcg cgcatgaagg cgcacacgtc
     3241 ttcgccaccg gccccgtcgt gcagctcggc gccgaagagg cggacgcctt cctctcgtcc
     3301 gcgctgctct ggttcgtgcg cgccgcggag cggcggcgcg acgcgcacgc gcac
//
LOCUS       DBNGOH010000246         4769 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1870016, whole genome shotgun sequence.
ACCESSION   DBNGOH010000246 DBNGOH010000000
VERSION     DBNGOH010000246.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4769)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4769)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4769
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1870016"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3034
                     /locus_tag="AC159P_04625"
     CDS             <1..3034
                     /locus_tag="AC159P_04625"
                     /inference="COORDINATES: protein motif:HMM:NF051698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HIR3694770.1"
                     /translation="ITLVLSQAAVVDVAMKVGSVQEVITVTENASLLNTATAEVGTRF
                     DSRRVSELPLSTNRNVYNIALSAPGVSQLGSGQTSFAGGGTGSTSGISFSANGGRIRS
                     NNFMIDGQDNNDFGVAGATIPLNNPDAIQEVRLVTNQFSAEYGRNSSAVFNAITKSGT
                     NDWHGSAFWFHNDNKLNACSNTNKAGGFCNPNATDPSRRGAPFRIENQLGGTIGGPLH
                     LPRFGEGGPSILSGKDRTFFFFSIQRWWDRQLGVGTTLNGAPTEAGRQVLAAAAGDLP
                     QVRALLDFLPAAQTPINRSAVFERNGQRFTVPLGSLTGSQPSAFNDWQTSFRIDHKLN
                     AKHNLNGRYLYQDNDTLGGPASQITPPGFSSAIVARTQGINTSLTSVLTSNLVNEARG
                     AYLRFASQTSALNPSAELIPSIEIVELGLRQFNAAATRTGIGLGVNLPQQSVRNTYQL
                     QDTVSYTTGNHGIKFGADIRRNQLHQLFKPTTRGLVEYGTLDFFVKDFATRFNINKDL
                     AGVARILHLDWHDFYFFAQDEWKVRPNFTLSFGLRYENPGQPIQDLVKFNEPVLAAAG
                     NDPRFRVTPIPGRDNDNIQPRLGFNWNPRTEGGPFNFLTGGDKLVVRGGYTRTHDYAF
                     TNIALNIWSSFPFVAAVSAIPTINAVDINAAAGAPLVRVSPNAFALLSNPPFNPDTLN
                     RTIVDEGFRTPSYDSFSLEMQRELTSDVVMRVGYVGTKGNGLFESVDANPTVIGCTNP
                     SAANNFCRTIPNQGPTRLRTNSGSSIYHSLQTSLEKRLSRGFSAGLHYTWSTFIDTQS
                     EIFNISTGEIAVAQDSYCRACDRARSSFDRPHRLSGNFVYELPWYREQRGFVGHVLGG
                     WQTNAFFTLQSGSPFTPLLGADPTGALASISGLVGNSIRPNLNTTLDLPRMSVEEIVA
                     AGGRTLFRGLSAGQRVGNAGRNILRSDGINNIDFGILKNTRLGEGHNLQIRADFFNLT
                     NSRDFGIPASDITSANFLNQWGTNGGNRRVIVGLRYVF"
     gene            3353..3793
                     /locus_tag="AC159P_04630"
     CDS             3353..3793
                     /locus_tag="AC159P_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008030201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HIR3694771.1"
                     /translation="MRVMVIIKANEVSEAGVLPGEKMLAEMGNFNEELVKAGVMLAGE
                     GLHPSSKGKRVRFSGTQKTVIDGPFAETKELIAGFWLWQVRSMEEAVEWVKRIPAPNE
                     GDPGYGEVSEIEIRQVFEASDFGAEFTPELREQEERLRAQIEEQ"
     gene            3858..>4769
                     /locus_tag="AC159P_04635"
     CDS             3858..>4769
                     /locus_tag="AC159P_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR3694772.1"
                     /translation="MTANDTHRTIEAVWRIESARLIAGLARIVRDVGLAEELAQDALV
                     AAFEQWPESGVPDKPGAWLMAAAKHRALDLLRRRKMLERKHEELGRELEARERAVPDF
                     DAALDEDIGDDLLRLVFIACHPVLSTEARVALTLRLLGGLTTGEIARAFLVAEPTIAQ
                     SIVRAKRTLSEARVPFEVPRGAELEARLSSVLEVIYLIFNEGYLATAGDDLMRPALCE
                     EALRLGRILAELAPSEPEVHGLVALMEIQASRLRARSGPKGEPILLLEQDRGRWDRLL
                     IRRGIAALERADKLGGASGPYALQAAIA"
BASE COUNT          861 a         1703 c         1468 g          737 t
ORIGIN      
        1 catcacgctc gtcctcagtc aggcggcggt cgtggacgtg gcgatgaagg tgggcagcgt
       61 gcaggaggtc atcaccgtca cggagaacgc ctcgctcctg aacacggcga cggccgaggt
      121 cggcacgcgc ttcgactcgc ggcgcgtctc ggaattgccg ctctcgacca accgcaacgt
      181 ctacaacatc gcgctctcgg cgccgggcgt cagccagttg gggagcggcc agacctcttt
      241 cgcgggcggc ggcaccggct ccacctcggg catcagcttc tcggccaacg gcgggcgcat
      301 ccgctcgaac aacttcatga tcgacgggca ggacaacaac gacttcggcg tcgccggggc
      361 gaccatcccg ctcaacaacc ccgacgccat tcaggaagtc cggctcgtga ccaaccagtt
      421 cagcgccgag tacgggcgca actccagcgc cgtcttcaac gccatcacca agtcgggcac
      481 caacgactgg cacggctcgg ccttctggtt ccacaacgac aacaagctga acgcgtgcag
      541 caacaccaac aaggccggcg gcttctgcaa cccgaacgcg accgacccgt cgcgccgcgg
      601 cgccccgttc cgcatcgaga accagctcgg cggcaccatc ggcggcccgc tccacctgcc
      661 gcgtttcggc gagggcggcc cctccatcct cagcggcaaa gaccgcacct tcttcttctt
      721 ctctatccag cgctggtggg atcgccagct cggcgtcggc accactttga acggcgcgcc
      781 gaccgaagcc ggtcgccagg tgctcgccgc ggcggcgggc gatttgccgc aggtgcgcgc
      841 gctcctcgac ttcctcccgg cggcgcagac gcccatcaac cgcagcgccg tcttcgagcg
      901 caacgggcag cgcttcaccg tcccgctcgg ctccttgacc ggctcgcagc cctccgcctt
      961 caacgactgg cagacctcct tccgcatcga ccacaagctc aacgcgaagc acaacctcaa
     1021 cggtcgttat ctctatcagg ataacgacac cttgggcggc cccgcttcgc agatcacgcc
     1081 gccgggcttc tcctcggcca tcgtcgcgcg cacgcagggc atcaacacct cgctgacgag
     1141 cgtgctcacg tcgaacctcg tcaacgaggc gcgcggcgca tacctgcgct tcgcctcgca
     1201 gacgagcgcg ctcaacccga gcgccgaact gattccttca atcgagatcg tcgaactcgg
     1261 cctgcgtcag ttcaacgccg ccgccacgcg caccggcatc ggcctcggcg tcaacctgcc
     1321 gcagcagtcc gtgcgcaaca cctaccagtt gcaggacacg gtctcttaca cgaccggcaa
     1381 ccacggcatc aagttcggcg ccgacatccg ccgcaaccag ctccaccagc tcttcaagcc
     1441 gacgacgcgc ggcctcgtcg agtacggcac gctggatttc ttcgtgaagg acttcgccac
     1501 ccgcttcaac atcaacaaag acctcgcggg cgtcgcgcgc atcctgcacc tcgactggca
     1561 cgacttctac ttcttcgcgc aggacgagtg gaaggtgcgc ccgaacttca cgctgagctt
     1621 cggcctccgc tacgagaacc cgggtcagcc catccaggac ctcgtcaagt tcaacgagcc
     1681 ggtgctcgcc gccgcgggca acgacccgcg cttccgcgtc acgccgatac cgggccgcga
     1741 caacgacaac atccagccgc gcctcggctt caactggaac ccgcgcacgg agggcggccc
     1801 cttcaacttc ctgaccggcg gcgacaagct cgtcgttcgc ggcggctaca cgcgcacgca
     1861 cgactacgcc ttcaccaaca tcgcgctcaa catctggagc agcttcccct tcgtcgcggc
     1921 ggtctcggcc atcccgacca tcaacgccgt ggacatcaac gccgcggcgg gcgccccgct
     1981 cgtccgcgtc tcgcccaacg ccttcgcgct cttgtccaac ccgcccttca acccggacac
     2041 gctcaaccgc accatcgttg acgaaggctt ccgcacgccg tcctacgact cgttcagcct
     2101 ggagatgcag cgcgagctga cgagcgacgt cgtgatgaga gtcggctacg tcggcacgaa
     2161 gggcaacggc ctgttcgagt ccgtggacgc caaccccacg gtcatcggct gcaccaaccc
     2221 gtcggcggca aacaacttct gccgcaccat ccccaaccag gggccgacgc ggctgcgcac
     2281 caactccggc agctccatct atcactcgct ccagacgagt cttgagaagc gtctctcgcg
     2341 cggcttctcg gcgggcctcc actacacctg gagcacgttc atcgacacgc agtcggagat
     2401 cttcaacatc tcgacgggcg agatcgccgt ggcgcaggac tcctactgcc gcgcgtgcga
     2461 ccgcgcgcgt tcctcgttcg accgcccgca ccgcctctcg ggcaacttcg tttatgagtt
     2521 gccctggtat cgcgagcagc gcggcttcgt cggccatgtc ctcggcggct ggcagacgaa
     2581 cgccttcttc acgctccaga gcggctcgcc cttcacgccg ctgctcggcg cagacccgac
     2641 cggggcgctc gccagcatct cgggcctcgt cggcaactcc atccgcccga acctcaacac
     2701 gacgctcgac ctgccgcgga tgagcgtcga agagatcgtc gcggcgggcg ggcgcaccct
     2761 cttccgcggc ctcagcgcgg ggcagcgcgt cggcaacgcg ggccgcaaca tcctgcgctc
     2821 ggacggcatc aacaacatag acttcggcat cctgaagaac acgcggctcg gcgagggcca
     2881 caaccttcag atcagggccg acttcttcaa cctcaccaac tcgcgcgact tcggcatacc
     2941 cgcgtcagac atcacgagtg cgaacttcct caaccagtgg ggcacgaacg gcggcaaccg
     3001 tcgcgtcatc gtcgggctga ggtacgtgtt ctaaaagtcg tccgacgctt gaaccccctt
     3061 gcggcggcaa agccatggcc gcttactggg gccggagaga acactctccg gccccaaaat
     3121 tttttccggc gaaagtgaac agagcagggg aaaaaggaca ggaagcctcc ccatatcgcg
     3181 gtcaagaccc tgccgagtcg gatggttgat aggataaaaa tatcgaaaaa agtgacgcga
     3241 ggtgtcgatt tcgcgccccg tcgttcgacg tgtatataga gcacgggtgg agccggacga
     3301 aaccgaccgg atgccctgtg cagaaccgga aaccataaag gagacaataa cgatgcgagt
     3361 catggtgatt attaaggcga acgaggtatc ggaggcgggc gtgctgccgg gcgagaagat
     3421 gctggccgag atgggcaatt tcaacgagga gctggtgaaa gccggagtga tgctcgcggg
     3481 cgagggtctc cacccgagtt ccaagggcaa gcgggtcagg ttctcgggca cgcagaagac
     3541 agtgatcgac ggccccttcg cggagacgaa ggagttgatc gcgggcttct ggctctggca
     3601 ggtgcgctcg atggaggagg ccgtcgagtg ggtcaagcgc atccccgccc ccaacgaggg
     3661 cgatccgggc tacggcgagg tatccgagat cgagattcga caggtgttcg aggcgtccga
     3721 cttcggcgcg gagttcacgc cggaactgcg ggagcaggag gagcgcctgc gcgcgcagat
     3781 cgaggagcag tagaagtctt gacggcgcgc gccctgcgcc ttgcgtccgc cgtttgttga
     3841 tggtgtgatg agtggccgtg acggccaacg acacacatcg caccatcgag gcggtctgga
     3901 ggatcgagtc ggcgcggctc atcgccggcc tcgcgcggat cgtgcgcgat gtcggcctcg
     3961 ccgaggagct ggcgcaggac gcgctcgtcg ccgccttcga gcagtggccg gagtcaggcg
     4021 tccccgacaa gccgggcgcc tggctcatgg ctgcggcgaa gcaccgcgcc ctcgacctgc
     4081 tgcgccggag gaagatgctc gagcgcaagc acgaggaact cgggcgcgag ttggaggcga
     4141 gggagagagc cgtgccggac ttcgacgccg cgctcgacga agacatcggc gacgacctcc
     4201 tgcgtctcgt cttcatcgcg tgccacccgg tgctctcgac cgaggcgcgc gtcgcgctca
     4261 cgctccgcct gctcggcggg ctgacgaccg gcgagatcgc gcgcgccttc ctcgtcgcgg
     4321 agccgaccat cgcgcagagc atcgtccgcg ccaagcgcac cctctccgag gcgcgcgtcc
     4381 ccttcgaggt gccgcgcggg gccgaactcg aagcgcgcct ctcgtccgtc ctcgaagtca
     4441 tttatctcat cttcaacgaa ggctacttgg cgaccgccgg agacgacttg atgcggcccg
     4501 cgctctgcga agaggcgctg cgcctcgggc gcatcctcgc ggagttggcg ccgtcggagc
     4561 cggaggtgca cggcctcgtc gcgctgatgg agatacaggc ttcgcgcctg cgcgcgcgga
     4621 gcggcccgaa gggagagccg atcctgctcc tcgaacaaga ccgcgggcgc tgggatcgac
     4681 tgctcatccg ccgcggcatc gcggcactcg aacgggccga taagctcggc ggcgcgtcgg
     4741 ggccgtacgc gcttcaggcc gccatcgcc
//
LOCUS       DBNGOH010000247         2600 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_219808, whole genome shotgun sequence.
ACCESSION   DBNGOH010000247 DBNGOH010000000
VERSION     DBNGOH010000247.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2600)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2600)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2600
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_219808"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..692)
                     /locus_tag="AC159P_04640"
     CDS             complement(<1..692)
                     /locus_tag="AC159P_04640"
                     /inference="COORDINATES: protein motif:HMM:NF013930.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHC2 zinc finger domain-containing protein"
                     /protein_id="HIR3694773.1"
                     /translation="MPDARAKEGRSEPPQERLRRLRDEIKRLVDYRAFYLRYCPDARQ
                     SGPRLQSLCPIPAHGHSGKGHPSLSVDLQHGLFNCFSRDEGGDAITFYERMHGVGFSR
                     AVEEMARELGLDGERKGRQSSLASRAAPDAEAADWEVLEPLDAERTSEVCERFLAVCR
                     GEDQLEGRNYLARRGIPAGVVARAGVAYFPRRAYRRVMRRMSGSFSVETLRRAGLFNR
                     KEHLTFYHHRLL"
     gene            complement(706..1386)
                     /locus_tag="AC159P_04645"
     CDS             complement(706..1386)
                     /locus_tag="AC159P_04645"
                     /inference="COORDINATES: protein motif:HMM:NF017885.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HIR3694774.1"
                     /translation="MDTERLPPTYKGRQYEIRAMGLREFAGLRRDEERLDPFELAYYA
                     KLMVIDFDQIEGLSEETKEHLLGVGTNDWSGGTASRPLPNGWRLVILNPKHGKARNNA
                     TLMEEVCHVFLGHKANRLSIVAQNKQGKTVARDYNEADEEAAYAVGAAALVPFAGLRA
                     LMKRGLTSIEIARHFNVSRELVEYRLKVSRLWSEYRARHPEEDAAREQRRRMFAASAA
                     TGDNGNAG"
     gene            complement(1523..2008)
                     /locus_tag="AC159P_04650"
     CDS             complement(1523..2008)
                     /locus_tag="AC159P_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR3694775.1"
                     /translation="MSEKVKAIPEGFHSVTPYLCVDGAARAIEFYKEAFGATERFRME
                     APGGKIGHAEIQVGDSIIMLADEMPEINFRSPHALGGPSSHFMIYVEDVDARVEQAVA
                     AGAKLTRPIKDEFYGDRTGCVQDPFGHLWYIATHVEDLTPEEINRRHEAEAAQAQQAG
                     A"
     gene            complement(2105..2464)
                     /locus_tag="AC159P_04655"
     CDS             complement(2105..2464)
                     /locus_tag="AC159P_04655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694776.1"
                     /translation="MTYKVIVRPEAEREIREAFDWYEERSEGLGLEFLRAADACLSGV
                     RRNPEAYPLVQEQARRALLQNSRTRFFTSSAIGQSLSSHVFTSGAALLIGNGASDGSM
                     RTETLILTLNGFASTVS"
     gene            complement(2461..2592)
                     /locus_tag="AC159P_04660"
     CDS             complement(2461..2592)
                     /locus_tag="AC159P_04660"
                     /inference="COORDINATES: protein motif:HMM:TIGR02574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module protein"
                     /protein_id="HIR3694777.1"
                     /translation="MPESLPLTDEQREELDRRLSLHARNPEEGIPWDELRERLRKSA"
BASE COUNT          415 a          886 c          821 g          478 t
ORIGIN      
        1 aagagcaggc gatggtggta gaaggtcaga tgctccttcc ggttgaagag accggcccgg
       61 cggagcgtct cgacggaaaa gctgccggac atgcggcgca tgacgcggcg gtaggcgcgg
      121 cgcgggaagt aggcgacccc tgcgcgcgcg acgacgcccg cggggatgcc gcgccgcgcg
      181 aggtagttcc tgccttcgag ctggtcctcg ccgcggcaga cggcgaggaa gcgctcgcag
      241 acctcgcttg tccgctcggc gtcgagcggt tcgaggactt cccagtccgc cgcttcggcg
      301 tcgggcgcgg cgcgcgaagc gagcgacgac tgacggcctt tgcgttcgcc gtcgaggccc
      361 agctcgcgcg ccatctcttc gaccgcgcgg ctgaagccca cgccgtgcat ccgctcgtag
      421 aaggtgatgg cgtcgccgcc ctcgtcgcgc gagaagcagt tgaagagtcc gtgctgaagg
      481 tctacagaga ggctggggtg gcccttgccg ctgtggccgt gcgcggggat ggggcagagc
      541 gattgaagac gcgggccgga ctgcctggcg tcggggcagt agcggaggta gaaggcgcgg
      601 tagtccacca gccgcttgat ctcgtcgcgc agacgacgca gccgctcttg cggcggctcc
      661 gaacgtccct cctttgcgcg cgcgtccggc atctcggttc ccgactcaac cggcgttgcc
      721 gttgtcgccc gtcgcggcgg aggccgcgaa catcctgcgc ctctgctcgc gtgcggcgtc
      781 ctcttccggg tggcgcgcgc ggtactctga ccacaggcgc gacaccttca gacggtactc
      841 gaccagctcg cggctgacgt tgaagtggcg cgcgatctcg atggaggtca gcccccgctt
      901 catcaacgcg cgcaaccccg cgaacggcac gagcgccgcc gcgccgaccg cgtaggccgc
      961 ctcctcgtcc gcctcgttgt agtcgcgcgc gaccgttttg ccctgcttgt tctgcgcgac
     1021 gatggagagc cggttcgcct tgtggccgag gaagacgtgg cagacctcct ccatcagcgt
     1081 cgcgttgttg cgcgccttgc cgtgcttcgg gttcaggatg acgagccgcc acccgttcgg
     1141 cagagggcgc gacgccgtgc cgcccgacca gtcgttcgtg ccgacgccca gcaggtgctc
     1201 tttggtctcc tccgacagcc cctcgatctg gtcgaagtcg atcaccatca gcttcgcgta
     1261 gtaggccagc tcgaacgggt cgagccgctc ctcgtcgcgg cgcaggcccg cgaactcgcg
     1321 caaccccatc gcgcgaatct cgtactgtcg gcccttgtag gtcggcggca atctctccgt
     1381 gtccacgtcg cgattataac ctccggcgcg ggccgtttca ctgcgaaact tcacacgtac
     1441 agccgcccct cgctcgttgt tgaaatagcg agggggtcgg cctcgacact cgcagccgac
     1501 cccctcgctc cgctgataac gcttacgccc cggcctgttg tgcctgcgcc gcctcggctt
     1561 cgtggcgccg gttgatctct tcgggcgtca ggtcttcgac gtgcgtggcg atgtaccaca
     1621 gatggccgaa ggggtcttgg acgcagcccg tgcggtcgcc gtagaactcg tccttgatgg
     1681 gccgcgtcag cttcgccccg gcggcgacgg cctgctcgac gcgcgcgtcc acgtcctcga
     1741 cgtagatcat gaaatgagac gacgggccgc cgagcgcgtg cgggctgcgg aagttgattt
     1801 ccggcatctc gtcggcgagc atgatgatcg agtcgccgac ctgaatctcc gcgtgcccga
     1861 tcttcccgcc cggcgcctcc atccggaacc gctcggtcgc cccgaacgcc tccttgtaaa
     1921 actcgatggc ccttgccgcc ccgtccacac acaggtacgg cgtcacgcta tgaaacccct
     1981 ccggaatcgc tttcaccttc tccgacatag tctctccttt acagcttcga aatctgatcg
     2041 ggagtttcag acgaacgcca gagcaacgac cgtcaccccg gcccacagcg aggcgtctat
     2101 ggtttcaaga aaccgttgaa gcaaacccgt tcaatgtgag tatcagagtc tctgttcgca
     2161 tcgaaccatc agacgcgccg ttgccaatca gcagggctgc gcctgatgtg aaaacatgcg
     2221 aggacaacga ttgtccgatc gctgacgacg taaaaaagcg cgtacgggaa ttttgcagaa
     2281 gcgcgcggcg ggcctgctct tgtacgagcg ggtaagcttc cgggttacgc cgcacgccgg
     2341 acagacaggc atccgccgct cgcaggaact ctagtccaag cccttcgctg cgctcctcgt
     2401 accaatcgaa ggcttcccga atctcgcgct ctgcttcagg gcggacgatg actttatacg
     2461 tcatgcggac ttccgcagcc tctctctcaa ctcatcccat ggaataccct cttccggatt
     2521 ccgtgcgtgc aacgataagc gccggtcaag ttcttctctc tgctcgtccg tcaggggcag
     2581 agactcgggc acggcggcga
//
LOCUS       DBNGOH010000248         3992 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3152865, whole genome shotgun sequence.
ACCESSION   DBNGOH010000248 DBNGOH010000000
VERSION     DBNGOH010000248.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3992)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3992)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3992
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3152865"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..393)
                     /locus_tag="AC159P_04665"
     CDS             complement(<1..393)
                     /locus_tag="AC159P_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013008510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HIR3694778.1"
                     /translation="MPTREDAWELLCEYTKGKSLRQHALAVEAAMRAAAARYGGPEED
                     VDEWGLVGLLHDFDYEMFPSADEHPYKGANILCGRGYSERFIRAIMGHATYTGVPRDT
                     ALARALFATDELCGFLVACALVRPSKSLD"
     gene            560..763
                     /locus_tag="AC159P_04670"
     CDS             560..763
                     /locus_tag="AC159P_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017652359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HIR3694779.1"
                     /translation="MTFTIEFEQETDGRWLAEVLELPGALAYGQTSAEAVAKVKALAL
                     RVLADRLEHGEATPELSNISFAA"
     gene            760..972
                     /locus_tag="AC159P_04675"
     CDS             760..972
                     /locus_tag="AC159P_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017652358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HIR3694780.1"
                     /translation="MSQWPSEKAKRVLAALLRIGWEVRRESGSHKVLSRPGWPDVTFA
                     FHDKEEIGPRMLARLAKNTGLKPEDL"
     gene            1053..1868
                     /locus_tag="AC159P_04680"
     CDS             1053..1868
                     /locus_tag="AC159P_04680"
                     /inference="COORDINATES: protein motif:HMM:NF013725.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C assembly family protein"
                     /protein_id="HIR3694781.1"
                     /translation="MKPFLYAALAAYVVAAIHSILAFLNKRRAVERVSFFALLAGFAA
                     HTVAILLDWVEDGHYPLFHLHETISFLAWTLVIAYALAVYRYRTRALGAFLLPAVSLL
                     VFASNAVRGGAGALASRDTDGSAAWLFPVHTTLLLFAYASFFVAFAACVMYLWQEREL
                     KLKTFGAAFHRLPPLSTVDDIGTTAAGIGFTLLTLGIVTGVVWSSARTGRMWHNDPIE
                     FFALLTWVLYLALLHYRNQWRGRKAAWAGVAGFCLVLCTVLGAVLLGGYHVFG"
     gene            2055..3323
                     /gene="hemA"
                     /locus_tag="AC159P_04685"
     CDS             2055..3323
                     /gene="hemA"
                     /locus_tag="AC159P_04685"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HIR3694782.1"
                     /translation="MSIVLVGLNHKTAPVELRERLAFTDAACAEGLRALVDGELVREG
                     LIVSTCNRVEVLAASAPEREAEAALRIGDFLSRERAIPADFFSSHLYKHADDDAVRHV
                     FRVASSLDSMVVGEPQVLGQVRRAYTVAVEAGTAGRVLHRLVHTALRVAKRVRTETGI
                     AASAVSISFTAVELGRKIFGSLEGATVLLIGAGEMAELAARHLSSVGASRVLVTNRTD
                     EAAQKLAGKFGGVAVGFDSLAASLAEADVVICSTAADTYVVTPEMALAARQARRNRPA
                     FYIDISVPRNVDPKVGELDNLFVFDVDDLEQVVASNIREREREAERAELIVESEVMRF
                     QQSLRNLDIGPAVGALREKMHAIAQGEFERQRQRLGDLTPEQERAVRALLTSAVNKIA
                     HPVIHRMRRTYDTGEDENLRAWRDIFKLEE"
     gene            3410..>3992
                     /gene="hemC"
                     /locus_tag="AC159P_04690"
     CDS             3410..>3992
                     /gene="hemC"
                     /locus_tag="AC159P_04690"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HIR3694783.1"
                     /translation="MIVIGSRGSKLALWQAEWVRSRLEAAGAEARVEVIKTEGDVRRD
                     VPLAVIGGKGVFTKEIEQALLEGRIDVAVHSLKDLPTIVPEGLTIAAVAEREDARDAL
                     VLRADKVHEGAAVKSLPAGACVGTSSQRRLAQLKHARPDLVVKDLRGNVDTRLRKLDG
                     DWYDAVILAAAGLRRLGFEGRIGAYIPVEEMLPA"
BASE COUNT          635 a         1329 c         1340 g          688 t
ORIGIN      
        1 gtccaagctc ttcgacgggc ggacgagcgc gcacgcgacg agaaagccgc acagctcgtc
       61 cgtggcgaaa agcgcgcggg ccagcgcggt gtcgcgcggc acgcccgtgt aggtcgcgtg
      121 gcccatgatg gcgcggatga agcgctcgga gtagccgcgc ccgcagagga tgttcgcgcc
      181 cttgtagggg tgctcgtccg ccgacggaaa catctcgtag tcgaagtcgt ggagcagccc
      241 gaccagcccc cactcgtcaa catcctcttc ggggccgccg tagcgcgccg ccgccgcgcg
      301 catcgccgcc tcgaccgcca gcgcgtgctg ccgcaggctc tttcccttcg tgtactcgca
      361 cagcagttcc caggcgtcct cgcgcgtcgg catggccgta accgtcccct tcgaggttgg
      421 aaagtctttt cggagctgaa gcgaccgtta gtttctcatt taaggaacgc actttcaacg
      481 ccgggcgcgt cggcttcccg tcgagcgagg tgtgttatta tcggcgacaa agcagcgggc
      541 cgaacaggtg tgcaagaaga tgaccttcac tatcgaattc gaacaggaga ctgacgggcg
      601 ttggctcgca gaggtgcttg agcttcccgg cgctctggct tacggacaaa cttccgccga
      661 agcagtggca aaagttaaag ccctcgcctt gcgcgtgctg gcagataggc tcgaacacgg
      721 cgaggcaaca cccgagttga gtaacatctc gttcgcggca tgagtcagtg gccttcggag
      781 aaagcgaagc gcgtgctggc cgcgctcctg cgcatcggct gggaggtcag gcgcgaatcc
      841 ggctcgcaca aagtcctttc gcgtccgggc tggcccgacg tgacgttcgc gtttcacgat
      901 aaagaagaga tcggcccgcg catgctcgcc cgcctcgcca agaacaccgg cctgaagccc
      961 gaagatttgt agcccgttcc gagacgaact tgccgccgct tttcttcctc aattagaatg
     1021 acgcgcgtac acgctgagca gttgttgcgc cgatgaagcc atttctttac gcagcgctcg
     1081 cggcctacgt cgtggccgcc atccactcga tcctggcgtt cctgaacaag cgccgggcgg
     1141 tcgagcgcgt ctcgttcttc gcgctgctgg cgggcttcgc cgcgcacacc gtggcgatcc
     1201 tgctcgactg ggtcgaggac gggcactacc ctctctttca cctgcacgag acgatctcgt
     1261 tcctcgcgtg gacgctcgtc atcgcctacg cgctggccgt ctaccgctac cgcacgcggg
     1321 cgctgggggc gttcctgctg ccggcggtct cgctgctggt gttcgcgtcg aacgccgtgc
     1381 gcggcggcgc gggcgcgctc gccagccgcg acacggacgg gagcgccgcg tggctcttcc
     1441 ccgtccacac gacgctgctg ctgttcgcct acgcctcgtt cttcgtcgcc ttcgccgcct
     1501 gcgtgatgta tctctggcag gagcgcgagc tgaagctgaa gaccttcgga gcggccttcc
     1561 accgtctgcc gccgctctcg accgtggacg acatcgggac gaccgccgcc ggcatcggct
     1621 tcacgctgct gacgctcggc atcgtcaccg gcgtcgtctg gtcttcggcc cgcacggggc
     1681 ggatgtggca caacgacccc atcgagttct tcgcgctgct cacgtgggtg ctctacctcg
     1741 cgctgctcca ctaccgcaat cagtggcgcg ggcgcaaggc ggcgtgggcc ggcgtcgcgg
     1801 gcttctgcct cgtcctctgc accgtcctcg gcgcggtgct cttgggcggc tatcacgttt
     1861 ttggatagta ggcagtaagc agcaagcagt aggcagtcaa agacggatga attgacgacg
     1921 atgcagacct ttcattcgag caaacatgac gcatccgggg cggcgacctt taccgtatat
     1981 cgtcgtgatg gagccgttat aagcaaggcg gttttgctgc ctaccgccta ctgcctactg
     2041 cctacttttt aaccatgtcc atcgttctcg tcggactcaa ccacaagacg gctcccgtgg
     2101 agctgcgcga gcgcctggcg ttcaccgacg cggcgtgcgc cgagggcttg cgcgctctgg
     2161 tggacggcga gctggtgcgc gaggggctga tcgtctcgac gtgcaaccgc gtggaggtgt
     2221 tggccgcctc tgcgcccgag cgcgaggccg aggccgccct gcggatcggg gacttcctca
     2281 gccgcgagcg cgccatcccg gcggactttt tcagcagcca cctctacaag cacgcggacg
     2341 acgacgccgt gcgccacgtc ttccgcgtcg cctcttcgct cgactcgatg gtggtcggcg
     2401 agccgcaggt cctggggcag gtgcgccgcg cctacacggt cgccgtcgag gcgggcacgg
     2461 cggggcgcgt cctgcaccgc ctcgtccaca cggccctgcg cgtagccaag cgcgtccgca
     2521 ccgagaccgg catcgccgct tcggcggtct cgatcagctt cacggcggtc gagctggggc
     2581 gcaagatttt cggctcgctc gaaggcgcga ccgtgctcct catcggcgcg ggcgagatgg
     2641 ccgagctggc cgccaggcac ctttcttcgg tgggcgcctc gcgcgtgctc gtcacgaacc
     2701 gcacggacga ggcggcgcag aagctcgccg ggaagttcgg cggcgtggcc gtcggcttcg
     2761 actcgctcgc ggcgagcctc gccgaggccg atgtcgtcat ctgctcgacg gctgccgaca
     2821 cctacgtcgt cacgccggag atggcgctcg cggcgcggca ggcgcggcgc aaccgcccgg
     2881 ccttctacat cgacatcagc gtgccgcgca acgtggaccc gaaggtgggc gagctggaca
     2941 acctcttcgt cttcgacgtg gacgacctcg aacaggtcgt cgcctcgaac atccgcgagc
     3001 gcgagcgcga ggccgagcgc gccgagctga tcgtcgagtc cgaggtgatg cggttccagc
     3061 agtcgctgcg caacctcgac atcggcccgg cggtcggcgc gctgcgcgag aagatgcacg
     3121 ccatcgcgca gggcgagttc gagcggcagc gccaacgcct cggcgacctc acgcccgaac
     3181 aggagcgcgc cgtccgcgcg ctgctcacct ccgccgtcaa caaaatcgcg caccccgtca
     3241 tccaccgcat gcgccgcacc tacgacaccg gcgaggacga aaacctgcgc gcgtggcgcg
     3301 acattttcaa actggaagaa tgaaagaagg gtgttgggtg ttgggtgttg ggtaagacac
     3361 atgcttttac ccaacaccca acacccaaca cccaacaccc aacaccctta tgatcgtcat
     3421 cggttcgcgc ggctcgaagc tggcgctgtg gcaggcggag tgggtgaggt cgcggcttga
     3481 ggcggcgggc gcggaggcgc gcgtcgaggt catcaagacc gagggcgacg tgagaaggga
     3541 cgtgccgctc gcggtgatcg gcggcaaggg cgtgttcacg aaggagatcg agcaggcgct
     3601 gctcgaaggc cggatcgacg tggccgtcca ttcgctgaag gacttgccga caatcgtgcc
     3661 ggaagggctg acgattgcgg cggtggccga gcgcgaagat gcgcgcgacg cgctcgtgct
     3721 ccgcgcggat aaggtgcatg aaggcgccgc ggtgaagagt ctcccggcgg gcgcgtgcgt
     3781 cggaacgtcg agccagcggc ggctggcgca gttgaagcat gcgcggcccg acctcgtcgt
     3841 gaaggacttg cgcgggaacg tggacacgcg tctgcgcaag ctcgacggcg attggtacga
     3901 cgcggtgatt ctcgcggcgg cgggcctgcg gcggctcggc ttcgaggggc gcatcggcgc
     3961 ttacatcccc gtcgaggaga tgctgcccgc cg
//
LOCUS       DBNGOH010000249         2335 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3519422, whole genome shotgun sequence.
ACCESSION   DBNGOH010000249 DBNGOH010000000
VERSION     DBNGOH010000249.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2335)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2335)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2335
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3519422"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..540)
                     /locus_tag="AC159P_04695"
     CDS             complement(<1..540)
                     /locus_tag="AC159P_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694784.1"
                     /translation="MEGVGRLPLPVWLLEQEARALLSRLARVKPFALLEPALPAATIP
                     PNAQTAIETFLASGRHELRARVRGFIAWLRSPPGFGASPAEAQRRFTLLRLRFNDVLS
                     QFDLFADVLTQRSEHETGVWLAGLDVVAAEALRVPGGYFDLPPVICYLDRGPGAAIRR
                     ARTRLPGGAENPVAIVRARP"
     gene            complement(599..1372)
                     /locus_tag="AC159P_04700"
     CDS             complement(599..1372)
                     /locus_tag="AC159P_04700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694785.1"
                     /translation="MTMHDLDRTQSYYETEFEPEAYAYEEEFADESDFEFGDTEMALD
                     EVEEMELAAQLLEITDEAEIDQFLGSVFKKVAKAGGGLLKKAVGGNLGQIAKGLIKKA
                     LPMAGGALGSLVAPGVGTAIGSKLGSAAGGIFGLELEGLSPQDQEYEVARRVVRLVGT
                     AAKKAQQLQSYFPPDSAARKAVIEAAKKHAPGLLQALGGFQKALGGFQFEAAPPQGAP
                     GRGPNGYAQQGAGGGQAGGQGVRSGRWVRHGRRIILLGV"
     gene            1977..>2335
                     /locus_tag="AC159P_04705"
     CDS             1977..>2335
                     /locus_tag="AC159P_04705"
                     /EC_number="3.1.12.1"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HIR3694786.1"
                     /translation="MGGENRPAGTLISRRHGLKGRPDYLLETAGGSIPVEIKLGGCPP
                     SGRPHGSHVMQLACYCLLCEEVAGASVPFGIIQYPDREVRVPFTPELRRGLLSLLGER
                     REAGAREAVHGNHAQRRR"
BASE COUNT          368 a          780 c          784 g          403 t
ORIGIN      
        1 cggtcgcgcg cggacgatgg cgacagggtt ctcggccccg cccggcagac gcgtgcgcgc
       61 ccgccgaatc gccgcgcccg gcccgcggtc gaggtagcag atcacgggcg gcaggtcgaa
      121 ataaccgccc ggcacccgca gcgcctcggc cgcgacgaca tccagccccg ccagccagac
      181 gccggtctcg tgctcgctgc gctgggtcag cacgtcggcg aacaggtcga actgcgagag
      241 cacgtcgttg aaacgcaggc gcagcaaagt gaacctgcgt tgtgcttccg ccggcgaagc
      301 cccaaagccc ggggggctgc gcagccaggc gatgaacccg cggacgcggg cgcgcaactc
      361 gtgtcggccc gaggcgagaa acgtctcgat ggccgtctgc gcgttcggtg ggatggtcgc
      421 ggctggcagc gccggctcca gcagggcgaa cggcttgacc cgcgccaggc gcgacagcag
      481 cgcacgcgcc tcctgctcga gcagccagac gggcaggggg agacgcccga cgccctccac
      541 gcctgttcga cctgcttccc gctcagactt gggtgctgac aaagtggcgc tgctcacgtc
      601 acaccccgag caggatgatc ctgcgtccgt ggcgtaccca ccgcccgctc cggacaccct
      661 gtcccccggc ttggccaccg ccggcgccct gctgcgcgta accgttcggc cccctgccgg
      721 gcgcgccctg cggcggggcg gcttcgaact ggaacccgcc gagcgccttt tggaacccgc
      781 cgagtgcctg gaggagtccc ggcgcgtgct tcttggccgc ctcgatcacc gccttgcgcg
      841 cggctgaatc aggcgggaag taggattgaa gctgctgcgc cttcttcgcg gcggtgccga
      901 cgagacgtac gaccctgcgg gccacttcgt actcctgatc ctgcgggctg agtccttcga
      961 gttcgaggcc gaagatgccg cccgcggcag aacccagctt gctgccgatg gccgtgccga
     1021 cgcccggcgc caccagactg cccagcgccc cgccggccat cggcagcgcc ttcttaatca
     1081 ggcccttggc tatttgaccg agattgccgc cgacggcttt cttcaacagc ccgccgccag
     1141 ccttcgccac cttcttgaac acactcccga ggaactggtc gatttccgcc tcgtccgtga
     1201 tttcgagcaa ctgggcggcg agctccatct cctcgacctc gtcgagcgcc atttccgtgt
     1261 cgccgaattc gaaatcggac tcgtccgcga attcctcctc gtacgcgtac gcctccggtt
     1321 cgaactccgt ctcgtagtaa ctttgcgtgc gatccaggtc gtgcatagtc atctccttct
     1381 taagtttgta gtgcgggctt cgagtcaggc tcgcgcgccg cgccctactg gattgcacgc
     1441 ggggtgccac ctctaagttg ttgataccgg gtggtgggtg gcgaagtaag tttcgccatg
     1501 cgctgggcgg ggcgtgattg ggcgaagacg agttcgcgtc accacgacgg tgcgaatcgg
     1561 accttcgtgt cgacggtcga agcgtgaagg ggtgacgaac ggggcgaagg ggccggaagg
     1621 acggacatcc cgcagagggg cgggccgctg aatttcgtca tgaccaaagc gagcgacaca
     1681 ccgctcgctg gagcctcggc gcgaggatgt cgtcgtcgcc tggcaccgag tggcatcgtg
     1741 tgcacggaag aacagtcgcg aacggacggc ggccgcgcgt cgtgacgatg atctgcgtag
     1801 tagcagccgc gttcttcagc ctgctactcc tcctttactt ggtgctccgt tgatagccat
     1861 ttgtctcacc ctattcgtca tcgctttatc actctgcgcg ctggccgcgg ggagggcggc
     1921 acgtagagag agagacaggg cggcgctctc gaaaggggaa gatcctgtac agcgatatgg
     1981 gcggggagaa ccgccctgcg ggaacgctca tctcgcggcg gcacggtctg aaaggtcgcc
     2041 ccgactatct gctcgaaacg gcgggagggt caatcccggt cgagatcaag ttgggtggct
     2101 gcccgccctc gggtcgtccc cacggctcgc acgtgatgca gctcgcctgt tactgcctct
     2161 tgtgtgagga ggtggcgggt gcttcggtgc ccttcgggat cattcaatac cccgacaggg
     2221 aggtgcgcgt cccgttcacg ccggagttgc gtcgcgggct actctcgctg ctcggggaga
     2281 ggcgggaggc cggtgcgaga gaagccgtac atggcaatca cgcgcagagg cggcg
//
LOCUS       DBNGOH010000250         6293 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_587344, whole genome shotgun sequence.
ACCESSION   DBNGOH010000250 DBNGOH010000000
VERSION     DBNGOH010000250.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6293)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6293)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6293
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_587344"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="AC159P_04710"
     CDS             <1..237
                     /locus_tag="AC159P_04710"
                     /inference="COORDINATES: protein motif:HMM:NF051698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HIR3694787.1"
                     /translation="GAFIGPNLRTVDISAVKSNRLPFLGEATVLQLRFEAFNVFNRAN
                     FAPPELRAFTPSFGRIRSTVTSSRQLQLGLRLSF"
     gene            343..795
                     /locus_tag="AC159P_04715"
     CDS             343..795
                     /locus_tag="AC159P_04715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694788.1"
                     /translation="MLWIIFALGAALAWGLYGPVLHKGQVQLGNPMRALLCVGAAYFL
                     IGVLVPVLTLSTGEGVSTGFTTKGATAATIGGALGALGAVCIIFAFRSGGLPTYVMPL
                     VFAGAPLVNVLFSMWLHPPKTAPNPLLYAGFVLAALGAGMVLYFKPQS"
     gene            complement(951..1166)
                     /locus_tag="AC159P_04720"
     CDS             complement(951..1166)
                     /locus_tag="AC159P_04720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694789.1"
                     /translation="MSDQESNSNGLLNFIAATVETMRDQMATKSDLARLESKMEAQFT
                     AVRGDIEQVLIRLRRRSVRAWIGSRPK"
     gene            complement(1221..2495)
                     /locus_tag="AC159P_04725"
     CDS             complement(1221..2495)
                     /locus_tag="AC159P_04725"
                     /inference="COORDINATES: protein motif:HMM:NF024753.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB
                     family protein"
                     /protein_id="HIR3694790.1"
                     /translation="MKSLLLALAFALAPLVHVAAGNWPQWRGPSANGVSDEKNLPSKW
                     GPEENVAWKLALPATTGTSGSTPIIWGDRVFLNVAEGDDLALWCVDKSKGTLLWKRPL
                     GSGNVKMRKHNMSSPSPVTDGRGVWVITGTGVLKSFDFAGRELWSRDVQKDYGAFGLN
                     WGYASSPLLHEDSLYIQVLHGMNTDDPSYVMRVEKKTGKTLWKVERPTAAERESPDSY
                     TTPALLRHGKTTEIVVTGGDVVTGHDPATGKELWRAGGLNPDNNPFYRIVASPIALDG
                     IVYAPTRVKPLLALRAGGRGDITKSHLVWSTPNGPDVPTPVTDGKYFYVVNDKGVMYC
                     LDAKTGKEIYGQQRLRAGTYSASPVLADGKLYATSEDGVTSVVRAGEKFEVLAENDLK
                     EYTLSSPAISDGQIFLRTTGHLYAVGKRGGTR"
     gene            2596..3117
                     /locus_tag="AC159P_04730"
     CDS             2596..3117
                     /locus_tag="AC159P_04730"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3694791.1"
                     /translation="MTSSDFRVSVRPARDEDGEFILALVPRLMEFGPPPWYDAARMVA
                     TDLETIGGWLKESPAGTAFFVAEDDEGTRLGFIHLNSTVDYFTREEVGHVSDVVVAPG
                     AEGRGAGRALMAAAEEWARGRGYRLLTLNAFSRNTRARRLYERLGYGEDMVKYVKELR
                     RPEGPEPDRREGC"
     gene            3117..3539
                     /locus_tag="AC159P_04735"
     CDS             3117..3539
                     /locus_tag="AC159P_04735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694792.1"
                     /translation="MNRQRLLSALLRVAGACELFAFIAVVMPRSWMEASHAWLGMGAL
                     PEGAVVMFMIRQASYTYGMHGVSLFLLATDVVRFRPLVRLNGVSFLLAAPVFFLIDKT
                     SGIPAWWTLIDTLGCGTFGAALLWLTSGDGARHETAGL"
     gene            3594..4022
                     /locus_tag="AC159P_04740"
     CDS             3594..4022
                     /locus_tag="AC159P_04740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694793.1"
                     /translation="MKRTIAVLALLVSLCAPVPAQDKDKTGKIRIEADQNYPVLSTKR
                     IQTMEKELNEAAREGYRLLPRTIVYKEGFFTAEMVMVMERAPRSNKSYEYKLVRATKE
                     TSLHRKIDEAGAQGFAPITMVIIGEHVIITEKETEQKAGR"
     gene            complement(4161..>6293)
                     /locus_tag="AC159P_04745"
     CDS             complement(4161..>6293)
                     /locus_tag="AC159P_04745"
                     /inference="COORDINATES: protein motif:HMM:NF014717.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3694794.1"
                     /translation="TTPKAFVTIEAGQALWQTRFGKLTSMRVRPAGAASLDSARDAFR
                     ASLRGALDPARAGLGVFAARAEGLRASQGATNFGEYFLYFSFFLVVSALLLAALFFRL
                     GVEQRLREIGTLQAVGFPASRIRALFVGEGAALALAGSLLGLLGAYAYGWLVMHGLRT
                     WWVGAVGTTALTLTVSPTSLVLGALGGLVAALACVVWTLRRLARTSTRSLLAGTTSED
                     GGKREKGEEGKREEASRAARFPFSPFRLLTFSPAALAVVFGVVGLLLLGAAAGQVVGQ
                     VAGFFGGGSALLVALLCAQAAWLRRRAASPIRGSGWWPVSRLGVRNATHRPGRSVLCI
                     TLIAFAVFIIVAVDSFRKDDRAAALDRKSGSGGYALLAESMLPVVHDPNGEEGREALT
                     LGTSAGEARELEGVRFERFRVRPGDDASCLNLYQPRNPKIVAPTDEFLGAGRFAFQSS
                     LAESSEEKENPWLLLRREFADGAVPVIADANSLSYVLHLKLGEDFVLEREGAPVRLRV
                     VGALADSIFQSELLMAEANFKRLFPEQEGARMFLIDVPTAERAEAVAGTLEERLADFG
                     FDVTRTGERLANFHRVENTFLSTFQTLGGLGLALGTLGLAAVLLRNVLERRRELALLR
                     AVGYGKSHFALMVVAENALLLFCGLLTGTACALLAVAPVVASRGGQLPFASLGALLLA
                     VVASGMVASLLATVAALRSPLLASLRAE"
BASE COUNT         1011 a         2166 c         2093 g         1023 t
ORIGIN      
        1 ggcgccttca tcggccccaa cctgcgcacc gtggacatct ccgcggtcaa gagcaaccgc
       61 ctccccttcc tcggcgaggc gaccgtcttg cagctccgct tcgaggcctt caacgtcttc
      121 aaccgcgcca acttcgcacc gcccgagctg cgcgcgttca cgccctcgtt cgggcgcatc
      181 cgctcgaccg tcacctcctc gcggcagctt caattagggc tgcggctatc gttctgaata
      241 ccgtgaaccg tgaaccgtga accgtaaacc gtgataagaa aacaaccgtt tttcacccat
      301 cacggtttac ggtttacggt ttacggcctt tgaggtctct ccatgctctg gatcatcttc
      361 gccctcggcg cggcgctcgc gtgggggctg tacgggccgg tgctgcacaa gggacaggtg
      421 cagctcggca accccatgcg ggcgctcctg tgcgtcggcg cggcttactt tttgattggc
      481 gtgctggtgc cggtcttgac tctctcgacc ggcgagggcg tgtccaccgg tttcacgacg
      541 aagggcgcga ccgccgcgac catcggcggc gcgttgggcg ctctgggcgc ggtctgcatc
      601 atcttcgcgt tccgttcggg cggcctgccg acctacgtca tgccgctcgt cttcgcgggc
      661 gccccgctcg tcaacgtcct cttctcgatg tggctccacc cgcccaagac cgcgcccaac
      721 ccgctcctct acgcgggctt cgtcctcgcc gccctcggcg ccgggatggt gctctacttc
      781 aagccgcagt cgtgaggccg gacttaggat tttgtgtctg gaaagattgg gcgagcgacg
      841 cgcgggcgag cgcgggcgtc ttcagtcgcc cgcgctcggc ggctgcccga gcagccgcag
      901 catgtcgggc ttgtccttga cgagcaggta gacgacgctc cgcaggcggc tcacttcggt
      961 ctcgatccga tccatgcgcg cacggagcgc cttctcaatc gaatcaacac ctgctcgatg
     1021 tctccgcgga cggcggtgaa ctgcgcttcc atcttcgact caagacgcgc gaggtcgctc
     1081 ttggtcgcca tctggtcgcg catcgtctcg accgtggcgg cgatgaagtt gagaaggccg
     1141 ttactgttgg attcctggtc gctcatggct gttttcagcc tatcacagca cgggcggctt
     1201 tgctgcgcgt ccggctgacg tcaacgggtg ccgccgcgct tgccgacggc gtagaggtgg
     1261 ccggtggtgc ggaggaagat ttggccgtcg gagatggcgg gcgagctgag ggtgtactcc
     1321 ttgaggtcgt tctcggcgag cacctcgaac ttctcacccg cgcggacgac gctggtcacg
     1381 ccgtcctcgc tcgtcgcgta gagcttgccg tcggcgagca ccggcgaggc gctgtaggtg
     1441 cccgcgcgca ggcgctgctg gccgtagatt tccttgccgg tcttggcgtc gaggcagtac
     1501 atgacaccct tgtcgttgac gacgtagaag tatttgccgt ccgtgacggg cgtcggcacg
     1561 tccggcccgt tcggcgttga ccagacgagg tgcgacttgg tgatgtcgcc gcgaccgccc
     1621 gcgcgcaagg cgagcagggg cttgacgcgc gtcggcgcgt agacgatgcc gtcgagcgcg
     1681 atgggcgagg cgacgatgcg gtagaagggg ttgttgtcgg ggttgagtcc ccctgcgcgc
     1741 cacagctctt tgccggtcgc agggtcgtgg cccgtgacca cgtcgccgcc ggtgacgacg
     1801 atctcggtcg tcttgccgtg gcgcagcagc gcgggcgtcg tgtaggagtc gggcgactcg
     1861 cgctcggctg cggtcgggcg ctcgaccttc cacagcgtct tgcccgtctt cttttcgacg
     1921 cgcatcacgt aggaagggtc gtccgtgttc atgccgtgga gcacctgtat gtagagcgag
     1981 tcttcgtgga gcaggggcga ggaggcgtag ccccagttga ggccgaacgc gccgtagtcc
     2041 ttctgcacgt cgcgcgacca cagctcgcgc cccgcgaagt cgaagctctt gaggacgccc
     2101 gtgccggtga tcacccagac cccgcgcccg tcggtcacgg gcgacggcga ggacatgttg
     2161 tgcttgcgca tcttcacgtt gccgctgccc agcgggcgct tccagaggag cgtgcctttc
     2221 gacttgtcca cgcaccagag cgcgaggtcg tcgccctccg cgacgttgag gaagacgcgg
     2281 tcgccccaga tgatcggcgt cgagccgctc gtgcccgtcg tggccggcag cgcgagcttc
     2341 cacgcgacgt tctcctccgg cccccacttc gagggcaggt tcttctcgtc gctcacgccg
     2401 ttcgcggagg ggccgcgcca ctgcggccag ttgcccgccg cgacgtgaac gagcggcgcg
     2461 agagcgaaag cgagtgcgag cagcagactt ttcatcattt ccggtcttct cctgtcgtct
     2521 tggctgaagg ttgtggacga gagtataagc cgttcggcgc gacgatgacg agcggaattt
     2581 tcgaggtgcg cggcgatgac aagctcggac tttagagtga gcgtccggcc cgcgcgggac
     2641 gaggacggcg agttcatcct cgcgctcgtc ccgcggctga tggagttcgg cccgccgccg
     2701 tggtacgacg cggcgcggat ggtcgccacc gacctcgaaa ccatcggcgg ctggctgaag
     2761 gagtcgcccg cgggcaccgc gttcttcgtc gccgaagacg acgaaggcac gcgtctcggc
     2821 ttcatccacc tcaacagcac ggtcgattac ttcacgcgcg aagaggtcgg gcacgtctcg
     2881 gacgtggtgg tcgcgccggg cgcagagggg cgcggcgcgg gccgcgcgct gatggcggct
     2941 gccgaggagt gggcgcgcgg gcgcggctac cgcctgctca cgctgaacgc gttttcgcgg
     3001 aacacgcggg cgcggcggct ctacgagcga ctcggctacg gcgaggacat ggtgaagtac
     3061 gtgaaggagc tgcggcggcc cgaaggcccc gagcctgatc gacgagaggg atgctgatga
     3121 accgtcaaag actgctgtcg gcgctgctgc gcgtggcggg cgcgtgcgag ctgttcgcct
     3181 tcatcgcggt ggtcatgccg cgctcgtgga tggaggcgtc gcacgcgtgg ctcggcatgg
     3241 gcgcgctgcc cgagggcgcc gtcgtcatgt tcatgatccg gcaggcgtcc tacacctacg
     3301 gcatgcacgg cgtctcgctc tttctgctcg ccacggacgt ggtaaggttc cgcccgctcg
     3361 tccgcctcaa cggcgtctcg ttcctgctcg ccgcgcccgt cttcttcctc atcgacaaaa
     3421 cctccggcat ccccgcgtgg tggacactca ttgacacgct cggctgcggc accttcggcg
     3481 ccgcgctgct ctggctcacc agcggcgacg gcgcgcgaca cgagacggcg ggcctgtgat
     3541 atcctgctgc tccacgctcc gccttaccac acgttcgcag aaggagtttt aaaatgaaaa
     3601 gaactatcgc ggtgctcgcg ctgctggtct cgctgtgcgc gcccgtcccg gcgcaggaca
     3661 aggacaagac gggcaagatc aggatcgaag ccgaccagaa ctatccggtg ctttcgacca
     3721 agcgcatcca gacgatggag aaggagctga acgaggccgc ccgcgaaggc taccggctgc
     3781 tgccgcgcac catcgtctac aaggagggct tcttcacggc ggagatggtg atggtcatgg
     3841 agcgcgcgcc gcggtcgaac aagagctacg agtacaagct ggtgcgcgcg accaaggaga
     3901 ccagcctgca ccggaagatc gacgaggccg gggcgcaggg cttcgccccc atcacgatgg
     3961 tcatcatcgg cgagcacgtc atcatcacgg aaaaagagac cgagcagaag gccggacgct
     4021 gacgcgcgga agaagtgctg agtactgagt gctgagtgct gagttaaaac aatgacgatt
     4081 gatgagagag tcgctgtttg gactcgttac ggttgacggc tgacggctca cggtttttct
     4141 cagcactcag cacttcttcg tcactccgct cgcagcgaag cgagcagagg cgagcgcaag
     4201 gccgcgaccg tcgcgagcag cgaggccacc atccccgacg cgacgaccgc gagcagcagc
     4261 gcgccgagcg aggcgaaggg cagttgcccg ccgcgcgagg cgaccacggg cgcgaccgcg
     4321 agcagcgcgc aggccgtgcc cgtcagcagc ccgcagaaga gcaggagcgc gttctccgcg
     4381 acgaccatca gcgcgaagtg cgacttgccg tagccgaccg cgcgcagcaa cgccagctcg
     4441 cggcgcctct caagcacgtt ccgcaagagc accgccgcca gcccgagcgt cccgagcgcg
     4501 aggccgaggc cgccgagcgt ctggaacgtc gagaggaagg tgttctcgac gcggtggaag
     4561 ttcgccagcc gctcgcccgt ccgcgtcacg tcgaaaccga agtcggccag ccgctcttcg
     4621 agcgtccccg cgacggcctc ggccctttcc gccgtcggca cgtcgatcag aaacatgcgc
     4681 gcgccctcct gctccgggaa gagccgcttg aagttcgcct ccgccatcag cagctcgctc
     4741 tggaagatgc tgtccgcgag cgcgccgacc acgcgcagac gcacgggcgc gccctcgcgt
     4801 tcgagcacga agtcttcgcc gagcttcagg tgcagcacgt agctcaggga gttcgcgtcg
     4861 gcgatgaccg gcaccgcgcc gtccgcgaac tcgcgccgca agagcagcca cgggttctcc
     4921 ttctcctcgc tcgattcggc gagcgagctt tggaaggcga agcgaccggc gccgagaaac
     4981 tcgtccgtcg gcgcgacgat cttcgggttg cgcggttgat agaggttaag acagctcgcg
     5041 tcgtcgccgg gccggacgcg gaagcgctcg aagcggacgc cctccagctc gcgcgcctcg
     5101 cccgccgacg tgccgagcgt caaagcctcc ctcccctctt cgccgttcgg gtcgtgcacc
     5161 acgggcagca tcgactcggc cagcagcgcg taaccgccgc tcccggactt gcggtcgagc
     5221 gccgccgcgc ggtcgtcctt cctgaacgaa tcgaccgcca cgatgatgaa caccgcgaac
     5281 gcgatcagcg tgatgcacag cacgctccgg cccggcctgt gggtcgcgtt ccgcacgccc
     5341 aagcgcgaga ccggccacca cccgctccct cgaatcggac tcgccgcacg ccgccgcagc
     5401 cacgccgcct gcgcgcacaa caacgcgacc agcagcgccg acccgcctcc gaagaacccc
     5461 gccacctgcc cgacgacttg ccccgccgcc gcgccgagca gcagcagacc gaccacgccg
     5521 aacacaaccg ctagcgcggc gggggaaaag gttaaaaggc gaaaaggcga aaagggaaag
     5581 cgagccgcgc gtgacgcttc ttcccttttc ccctcttccc ccttttccct tttccccccg
     5641 tcttcgcttg tcgttccggc cagcaggctc cgcgtcgagg tgcgcgcgag gcggcgcagc
     5701 gtccagacga cgcaggcgag cgcggcgacg aggccgccca acgcgccgag cacgagcgac
     5761 gtgggcgaga ccgtgagcgt caaggccgtc gtccccaccg cgccgaccca ccacgtccgc
     5821 agcccgtgca tcacgagcca gccgtacgcg tacgcgccca agaggccgag gaggctgccc
     5881 gcgagcgcga gcgccgcgcc ctcgcccacg aagagcgcgc ggatgcgcga ggccgggaag
     5941 ccgacggctt gcagcgtccc gatctcgcgc aggcgctgct cgacgccgag ccggaagaag
     6001 agcgccgcca gcagcagcgc cgagacgacg aggaagaagc tgaagtagag aaagtattcg
     6061 ccgaagttcg tcgccccttg cgacgcccgc aacccttccg cccgcgccgc gaagacgccg
     6121 agtcccgcgc gcgcagggtc taacgcgccg cgcaaggaag cgcgaaacgc gtcgcgcgcc
     6181 gagtccagag acgcggcgcc cgccgggcgg acgcgcatcg aggtcagctt gccgaacctc
     6241 gtctgccaga gcgcctgccc cgcctcgatc gtcacgaacg ccttcggcgt cgt
//
LOCUS       DBNGOH010000251         3075 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4986214, whole genome shotgun sequence.
ACCESSION   DBNGOH010000251 DBNGOH010000000
VERSION     DBNGOH010000251.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3075)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3075)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3075
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4986214"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..239)
                     /locus_tag="AC159P_04750"
     CDS             complement(<1..239)
                     /locus_tag="AC159P_04750"
                     /inference="COORDINATES: protein motif:HMM:NF024884.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HIR3694795.1"
                     /translation="MLGTTVRRYAVAVWAIGFAFAATLLLSPVVPPEGSPLFLLAVMV
                     SAWRGGLGAGLLATLLSTLANVFVFLPPVFSLYVGR"
     gene            complement(374..1234)
                     /locus_tag="AC159P_04755"
     CDS             complement(374..1234)
                     /locus_tag="AC159P_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015351055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HIR3694796.1"
                     /translation="MSRPPNRSKSGVRAAEARPPSGGFRGRLHEVIFEADTRAGRAFD
                     IGLIGLIILSVLAVMLESVGGVRRRYGAQLHAAEWAFTILFTIEYALRLLSVRRPLRY
                     ASSFFGVVDLLAIVPTYLSLLVPGSHYLLIIRILRLLRVFRVLKLAEHLTEADVLMRA
                     LRASRRKISVFLLTVLTLVVIIGTLLYVIEGEANGFTSIPVGIYWAVVTLTTVGYGDI
                     SPKTPVGQTLAALVMIIGYGIIAVPTGIVTVEIAQAARKPVSTQACPACSSQGHDVDA
                     VHCKYCGAKL"
     gene            1519..2436
                     /locus_tag="AC159P_04760"
     CDS             1519..2436
                     /locus_tag="AC159P_04760"
                     /inference="COORDINATES: protein motif:HMM:NF023695.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3616 domain-containing protein"
                     /protein_id="HIR3694797.1"
                     /translation="MFLASVLCSLALSSCGGTPPPPGGQVLNSMTAFEGGKFDASGVA
                     AVPGTDGVLFVDNGREGEVCWMSLDRDGRQAGAIKPVGLGVGIEDLEGITTDGTYFYV
                     VSSQSKPKAIAKEGLVRFKFDARSQSVEAVEAISGLKSFLVENVAELRGEGDRKGKDG
                     GVNIEGLAWDTRRGRLLLGLRSPIVDGHALLVPLKLRDPRGAFSVDNLEVEGSKAIRL
                     SLGGVGIRGVEYDGRANVFRILSGATEEQEQTDFGLWEWNGDERQPVLRETNRFDNSL
                     KPEGVARVTVGSRDLMLVVFDASGYAVMD"
     gene            complement(2479..>3075)
                     /locus_tag="AC159P_04765"
     CDS             complement(2479..>3075)
                     /locus_tag="AC159P_04765"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MsnO8 family LLM class oxidoreductase"
                     /protein_id="HIR3694798.1"
                     /translation="LIGYLHDSLPADHPHARTRAMPDGVAAPEIWMLGSGGDSAELAA
                     RFGCAFSFAQFIHGADGAGAVRDYRQHFRPSIFLAEPTASAAVFVVCAETEAEAQLLA
                     SSGELWRLRIERGVERPFPPVEEARSHPYTEAETMRLAQLRARNVVGAPEQVKHKLLE
                     IAERYGVDELLLVTITHDFEARVRSYELIAQAFDSNRT"
BASE COUNT          551 a          972 c         1056 g          496 t
ORIGIN      
        1 cgtccgacgt agagcgagaa gacgggcggg aggaagacga agacgttggc cagcgtcgag
       61 agcagcgtgg cgagcagccc cgcgccgagg ccgccgcgcc acgcgctcac catgacggcc
      121 agcaggaaga gcggagaccc ttccggcggg acgaccggcg acagcagcag cgtcgccgcg
      181 aaggcgaagc cgatcgccca gacggcgacc gcgtatcgcc tcacggtggt gccgagcatc
      241 aacccgatct cccctcacac gtccaatcgt ccacccgccg tcgcgaatgt tcggcgacga
      301 ttatacaagc gctccggggg tcgcccacag acgaaacccg ctccgtcgcg gcggaacgtg
      361 ccgcgcgccg cgcctacagt ttggccccgc aatacttgca gtggacggcg tccacgtcgt
      421 gcccctgaga ggagcaggcc gggcaggcct gggtcgagac gggcttgcgc gccgcctgcg
      481 cgatctcgac cgtgacgatg cccgtcggca ccgcgatgat cccgtagccg ataatcatca
      541 ccagcgccgc cagcgtctgc cccacgggcg tcttgggcga gatgtcgccg tagccgaccg
      601 tcgtcagcgt gacgaccgcc cagtagatgc cgaccgggat gctcgtgaag ccgttcgcct
      661 cgccctcgat gacgtagagc agcgtcccga tgatgaccac cagcgtcagc accgtcagga
      721 ggaagacgct gatcttgcgg cggctcgccc gcagagcgcg catcagcacg tcggcctccg
      781 tcaggtgctc ggcgagcttg agcacgcgga agaccctcag cagccggaga attctgatga
      841 tgagcaggta gtggctgccg gggacgagga ggctcaggta ggtggggact atcgccagca
      901 ggtcaacgac gccgaagaag ctcgacgcgt agcgcagggg ccgccggacg ctcaggagcc
      961 gcagggcgta ttcgatggtg aagaggatgg tgaaggccca ctcggcggcg tggagctgcg
     1021 cgccgtaacg ccgtcgcacg ccgccgacgc tttcgagcat gacggccaac acgctgagaa
     1081 tgatgaggcc gatcagaccg atgtcgaagg cgcgccccgc ccgtgtgtcg gcctcgaaga
     1141 tgacctcgtg cagcctgccg cgaaacccgc ccgagggcgg gcgtgcctcg gcggcgcgga
     1201 cgccggactt acttcgattc ggagggcggc tcatggaggg ctgctgctta cggctgcgcc
     1261 ggaaagtctc cttcgttttt tttcagcttc caattcatca tacacgagga tgttgacagt
     1321 ctaccgaccc atgttccccc tgccgccgac atcctgcggc gagttccctt cacctcttcc
     1381 gcccgcgctc tcggcccggt tcctcgcccg cggttgattg cccgctcgac ccttcaggta
     1441 tttaccgccc gtttaccgtg gatttgataa tctgaatgga tgagacccac gttttcatat
     1501 ctcgatatcc ggcgagtgtt gtttctcgcg tccgtcctct gctcgttggc cctgtcgtct
     1561 tgcggcggga cgccgccgcc gcccggcggg caggtactca acagcatgac ggcgttcgag
     1621 ggcggcaagt tcgacgcctc cggcgtcgcc gccgtgcccg gaacggacgg agtgcttttc
     1681 gtcgataacg ggcgagaggg agaagtttgc tggatgagcc tcgaccggga cggcagacaa
     1741 gccggcgcga tcaaacccgt cggactcggg gtcggcatcg aagacctcga aggcatcacg
     1801 actgacggaa catatttcta cgtcgtcagc tcgcagtcca aaccgaaggc catcgccaaa
     1861 gaggggcttg tccgcttcaa gttcgatgcg cgtagtcaaa gcgtcgaagc ggtcgaagcg
     1921 atcagcgggt tgaagagctt tctcgtcgag aatgtggccg agctgcgcgg ggagggcgac
     1981 aggaagggca aagacggcgg cgtcaacatc gaagggctgg cctgggacac tcggcggggc
     2041 aggctgttac tggggttgcg ctcgcccatc gttgacggcc atgccttact cgtcccgctc
     2101 aagctgcgcg acccgcgggg cgccttctcc gtggacaatc tggaagtgga aggctccaaa
     2161 gcgatccggc tctcactcgg aggggtcggc atcaggggcg tcgagtatga cgggcgggcg
     2221 aacgtcttca gaatactcag cggggcgacg gaggagcagg agcagaccga tttcggtctc
     2281 tgggaatgga acggagatga acggcagccc gtgctccgag agacgaacag attcgacaac
     2341 agcttgaagc ccgaaggggt ggcgcgagtg acggtgggca gccgtgattt gatgctcgtc
     2401 gtctttgacg cgagcggata cgcggtgatg gactaggggc cgaagttccg gccctttatc
     2461 aacctgcgcg gggcggcgtc aggtgcgatt cgaatcgaat gcctgcgcga tcaattcgta
     2521 tgagcggacg cgggcctcga agtcgtgcgt gatggtcacg agcagcagct cgtcaacgcc
     2581 gtagcgctcc gcgatctcca gcagtttgtg cttcacctgc tcgggggcgc cgaccacgtt
     2641 gcgcgcgcgg agctgcgcga ggcgcatcgt ctccgcctcg gtgtacgggt ggctccgggc
     2701 ctcttcgacg ggcgggaaag ggcgctccac cccgcgctcg atacgcaaac gccacagctc
     2761 accgctggac gccagaagtt gcgcttccgc ctccgtctcg gcgcagacga cgaagacggc
     2821 ggcgctcgcc gtcggctctg caagaaaaat cgaggggcga aagtgctgcc ggtaatcgcg
     2881 cacggcgccg gcgccgtcgg ccccgtggat gaactgcgcg aaggagaagg cgcagccgaa
     2941 acgcgcggcg agttcggcgc tgtcgccgcc cgagccgagc atccagattt ccggggcggc
     3001 gacgccgtcc ggcatcgccc gcgtgcgcgc gtgcgggtgg tctgcgggca gcgagtcgtg
     3061 aaggtagccg atgag
//
LOCUS       DBNGOH010000252         2602 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_404417, whole genome shotgun sequence.
ACCESSION   DBNGOH010000252 DBNGOH010000000
VERSION     DBNGOH010000252.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2602)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2602)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2602
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_404417"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            550..1572
                     /locus_tag="AC159P_04770"
     CDS             550..1572
                     /locus_tag="AC159P_04770"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HIR3694799.1"
                     /translation="MSVCLGVGGRAQPRNPAPTPTPQDDDIVERVESNLVLLNVGVAD
                     QRGRAITDLSRADFVVYEDGVRQNITSFEPTTAPFSLVLLLDMSGSTLNFRPTLKQSA
                     IRFLDALAPDDRVAVVAFWRETKKKGDNRLEVLADFTNDRKEISFAITRAEGSGQTNL
                     YEGLRFALRQLAREGQRRKAVVVLTDGKDTELEDVDRKAAAAARTGEDALAAVKPEAS
                     TQLRAVLDAADRQGVTVYPLALPSGDIKRIADPTPQQVAIFAGARARLQSLADNTGGR
                     LHAINRLEDLGRLYAEVAAELRTLYSIAYQSSSTRRRDGSWRAVNIEVTRAQLIARSR
                     PGYYAR"
     gene            1671..1744
                     /locus_tag="AC159P_04775"
     tRNA            1671..1744
                     /locus_tag="AC159P_04775"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1705..1707,aa:Arg,seq:acg)
     gene            2110..2286
                     /locus_tag="AC159P_04780"
     CDS             2110..2286
                     /locus_tag="AC159P_04780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694800.1"
                     /translation="MDFDHREGEQKVFEMNRVSYVTMSALKREIEKCDVVCANCHRER
                     TYRRVMQRRASRLA"
     gene            2311..2580
                     /locus_tag="AC159P_04785"
     CDS             2311..2580
                     /locus_tag="AC159P_04785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694801.1"
                     /translation="MAVGFYVTLILAMTLACVAGVLYFYMMFLEARNRQAARRVAELE
                     RENAALRESLRRAEGAAGQGGGAEEDGEVWPDVIGDEEGDGYSLK"
BASE COUNT          472 a          881 c          811 g          438 t
ORIGIN      
        1 cgccgaggcg cgccgcacac gccgcacgag cagccggccc gccaaacagg agccgccccc
       61 gcccgtcccc ttccccgcgc ccgtccctgt cgaagacatg tcgaccgccg tcgccgcacg
      121 acccacgcgc cgccgcgccc gcgccgggaa ataagaaggg ggaaaagggg aaaagggaaa
      181 agggagaagg atgaagaggg aggatgagag gcagcgtgcc tttcgtcctc cctcttcccc
      241 tcttgacctt ttgacctttt gaccttctcg cctcttcccc ttttcccccg tttccgcaaa
      301 gcaactcgcc gcacgccgcg ggcacccaaa cagtccgtcg ccgttggaga gccgtggaag
      361 gcgcgtgaaa acatccgtcc ggattttttc cggcccctga gttttcccgt tgacggcggg
      421 gttggcttcg cttgatttcg gcccacgcgt ccctgcatac tgaagcgtcg tgcggtgttt
      481 tgagcaatga ttccttcgag ccttcttaac aaattctctc ccgcaaaact cgtctgcgcg
      541 gccttcgccg tgagcgtctg tctcggcgtc ggcgggcgag cgcagccgcg caatcccgct
      601 ccgacgccga cgccgcagga cgacgacatc gtcgagcgcg tcgagtcgaa cctcgtcctc
      661 ctcaacgtcg gcgtcgccga ccagcgtggc cgcgccatca cagacctgtc gcgcgcggac
      721 ttcgtcgtct acgaggacgg cgtgcggcag aacatcacca gcttcgagcc gacgaccgcg
      781 cccttcagcc tcgtgctgct gctcgacatg tccggctcga ccctgaactt ccgccccacg
      841 ctcaagcagt ccgccatccg cttcctcgac gcgctcgcgc ccgacgaccg cgtcgccgtc
      901 gtcgccttct ggcgcgagac caagaagaag ggcgacaaca ggctggaggt gctcgccgac
      961 ttcaccaacg accgcaagga gatcagcttc gccatcacgc gcgccgaagg cagcggccag
     1021 acgaacctct acgagggcct gcgcttcgcg ctgcgccagc tcgcgcgcga ggggcagcgg
     1081 cgcaaggccg tcgtcgtcct caccgacggc aaagacacgg agctggagga cgttgaccgc
     1141 aaggccgccg ccgccgcgcg cacgggcgag gacgcgctcg ccgccgtcaa gcccgaggcg
     1201 agcacgcagc ttcgggccgt gctcgacgcc gccgaccggc agggcgtcac ggtctacccg
     1261 ctcgccctgc cgtcgggcga catcaagcgc atcgccgacc ccacgccgca gcaggtcgcc
     1321 atcttcgccg gcgcccgcgc ccgcctccag tcgctcgcgg acaacacggg cgggcgcctc
     1381 cacgccatca accgcctcga agatttgggc cgcctctacg cagaggtcgc cgccgagctg
     1441 cgcacgctct acagcatcgc ctaccagtcg tccagcaccc gccggcgcga cgggagctgg
     1501 cgcgccgtca acatcgaagt cacccgcgcc cagctcatcg cgcgctcccg ccccggctac
     1561 tacgcgcgct gacgaaacgg agccgaacgc acgccctcgg gcgaccgatg tccgcttgac
     1621 actcgtcggc ctcgccctta tagttttggc ttcacttaac caaccagttt gcacccgtag
     1681 ctcagttgga tagagcgttt gactacgaat caaaaggtcg gaagttcgag tcttcccggg
     1741 tgcatcacac gtcgataaaa gaggctgaga cacagcctct tttgtttttc ttcccgcgtc
     1801 ccgccgtgtg ataaagttcc ctccatgcgg acaccgaatg atgtcttggc tgattggggg
     1861 ggcagagtgt gctcgacgtg taagctcgat aagccgatcg aagagttcga ttttcgtaac
     1921 cgttcaaccg ggtcagggca tggttactgc cgcgaatgcg ggaagatatt tacccggaac
     1981 cactacaggc agaacaaacg ccagtacgtc gagcggacac ttcgctcgca cgcgcggcgt
     2041 cgcgagtttt tacaccagac gaaatcacgg ccctgcgccg actgcggcgt tcagtaccct
     2101 tattacgtga tggacttcga tcaccgcgag ggagaacaga aagttttcga gatgaaccgc
     2161 gtcagctacg tgacgatgag cgcgctcaag agagagattg agaagtgcga tgtcgtctgt
     2221 gcgaactgtc accgagagcg cacgtaccgg cgcgtcatgc agaggcgggc gagccgtctc
     2281 gcctgaactc cgtccttctt tcgtcaggcg ttggccgtgg ggttttacgt cacactgatt
     2341 ctcgcgatga cgctggcctg cgtggcgggc gtgctctact tttacatgat gtttctggag
     2401 gcgcggaacc ggcaggcggc gcggcgggtg gcggagttgg agcgggagaa cgcggcgctg
     2461 cgcgagtcgt tgcggcgggc cgaaggcgcg gcgggacagg gcggcggggc ggaggaggac
     2521 ggcgaggtct ggcccgacgt gatcggcgat gaagaaggcg acgggtattc gttgaagtga
     2581 acagcaagaa cagcgggcag ca
//
LOCUS       DBNGOH010000253         2627 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3887843, whole genome shotgun sequence.
ACCESSION   DBNGOH010000253 DBNGOH010000000
VERSION     DBNGOH010000253.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2627)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2627)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2627
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3887843"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..917)
                     /locus_tag="AC159P_04790"
     CDS             complement(<1..917)
                     /locus_tag="AC159P_04790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694802.1"
                     /translation="MSLGQESTPNGATQLNCNERPCDAVARGRAAFNDRNLRQLGGNG
                     RACSDCHLPSDGFQLSPTAARARFDALQAARAHNPNADDPLFRPVDADDFRTNGEAAS
                     DYSNLLENGLVRVTMPLPPNVRIIDPATGHPTDETSVDLWRAVMPVANVAITGPDGVA
                     PLWPPAPRVPIMGTDPAGPNRQGGYQHDARFGTLQEQARGALLAHAQVAVEPPERMLD
                     DLAAFQRTLFSSPGVELLAAAIRSGTTPFPDPDPELSELEQQGKAVFARSCAQCHGGA
                     LHPSTSTSETAIVRPILERYHNIRASCPRP"
     gene            1139..>2627
                     /locus_tag="AC159P_04795"
     CDS             1139..>2627
                     /locus_tag="AC159P_04795"
                     /inference="COORDINATES: protein motif:HMM:NF012698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HIR3694803.1"
                     /translation="MKTYVGEPQPQIYEFGDFRLDTSERLLTARDGRVVPLTPKPFET
                     LLYLVEHAGKVLNKDELMRAVWPDTAVEENNLSQCISALRRALGEKPGQQRYIATVQG
                     RGFRFVAPVTARADEDGRGGAAGPTSGPGAVRSGAEETDPPRAARPAGGLGTNLKLVA
                     IASVLIAGVAGLYLWWGRTGTAPLTSPAPPAAQSTIPAAPAKSVAVLPFKPLAAEGRE
                     EALEVGMADTLIAKLSNIGGLTVRPLGAVRRYGGLEQDPLSAGRELGVEAVLDGTVQR
                     SGDRVGVMARLLSVGDGRQLWAGQFDAPFTDIFSVQDAISAQVAGSLRPRLTGTEERR
                     LAKHHTANVEAYQLYLRGRYHLLKNTRSEIQTAISHFRQAVEVDPSYALAYAGLANAY
                     HALAGPGEMPPTELLPQAKAAAQKAVEIDDGLAEAHALLGFVIFWYDWDWGAAEDQFR
                     RALELDPNSADAHYAYADLLTYTGRHAEALAEIRRARELDPLNLRI"
BASE COUNT          394 a          877 c          956 g          400 t
ORIGIN      
        1 gggcgcgggc aggaggccct gatgttgtgg tagcgctcca ggatcgggcg cacgatggcc
       61 gtttcggaag tagacgtgct cgggtgcagc gcgccgccgt ggcactgcgc gcaggagcgg
      121 gcgaagaccg ccttgccttg ctgttcgagc tcactcagct ccggatcggg gtctgggaag
      181 ggcgtcgtgc cggagcggat cgccgcggct agcagttcga cccccggcga ggagaagagc
      241 gtccgctgga aggccgcgag gtcgtcgagc atccgctcgg gcggctcgac cgcaacttgc
      301 gcgtgggcga ggagcgcgcc tctcgcctgc tcctggagcg tgccgaagcg ggcgtcgtgc
      361 tggtagccgc cctgccggtt cggccccgct gggtcagtac ccatgatggg gacacgcggg
      421 gcgggcggcc agaggggcgc gacgccgtcc ggccccgtga tggcgacgtt cgcgacgggc
      481 atgaccgccc gccagaggtc aaccgaggtc tcgtcggtcg ggtgccccgt ggcggggtcg
      541 ataattctga cgttgggtgg caggggcatc gtcacgcgga cgagcccgtt ctccaacagg
      601 ttcgagtagt cgctggccgc ctccccgttg gtgcggaagt cgtcggcgtc gaccgggcgg
      661 aagagcgggt cgtcggcgtt cgggttgtgc gcgcgtgcgg cctgcagcgc gtcgaagcgg
      721 gcccgcgcgg ctgtcggcga gagttggaag ccgtcggacg gcaggtggca gtcggagcag
      781 gcgcgcccgt tgccgccgag ctgtcggagg ttgcggtcgt tgaaggcggc gcgccctcgc
      841 gccaccgcgt cgcagggccg ctcgttgcag ttgagctgcg tcgcgccgtt cggtgtcgat
      901 tcttgcccga ggctcatgct cggcacaccg gcggcgatcg cgccggcgag tgtgaggagg
      961 cataagaggg ccttggctgc ggttcttcgg gtaagcatga cttgtctcct ttcgcgccgc
     1021 gtgcaagttc gtgctaactc tcaccggagt gtttgtcggg tcccgccgac gggcgccgat
     1081 actcttccca aaccctcctg aaaatcttta gaatgcgctt gaaaattttc tgaaaacctt
     1141 gaaaacgtac gtgggcgagc cacaacccca gatttacgag ttcggcgact tccgcctcga
     1201 cacctccgag cggctgctga ccgcacgcga cgggcgggtc gtgccgctca cgcccaagcc
     1261 cttcgagacg ctgctctacc tggttgaaca cgccgggaag gtactgaaca aggatgagct
     1321 gatgcgcgcg gtctggcccg acacggccgt cgaggagaac aacctcagcc agtgcatctc
     1381 ggcactgcgg cgcgcgctcg gcgagaagcc cggccagcag cgctacatcg cgacggtgca
     1441 agggcgcggc ttccgcttcg tcgcccccgt cactgcacgc gcggacgaag acggccgcgg
     1501 gggagcggcc ggaccgacgt ccggcccggg ggcggtgcgt tcgggggcgg aggagacaga
     1561 ccctccgcgg gcggcgcgtc ccgcgggcgg gctgggaacg aacctcaagc tcgtcgccat
     1621 cgcgtccgtg ctcatcgccg gcgtggcggg tctctacctc tggtgggggc gtacgggaac
     1681 ggcccccctc acttcgcccg cacctcccgc cgcgcagtcg acgatccccg ccgcgcccgc
     1741 caagtccgtt gcggtgctgc cgttcaagcc gctggcggcg gaggggcgcg aggaggccct
     1801 cgaggtcggg atggccgaca cgctcatcgc caaactcagc aacatcggcg ggctcacggt
     1861 gcgcccctta ggcgccgtgc gccggtacgg tggtctggag caggacccgc tttcggcagg
     1921 cagagagctc ggcgtggagg ccgtgctgga cgggaccgtc caacgctccg gcgaccgcgt
     1981 cggcgtgatg gcgcggctgc tgagcgtggg cgacgggagg cagctgtggg cgggacagtt
     2041 cgacgcgccc ttcaccgaca tcttctcggt gcaggacgcc atctcggcgc aggtggccgg
     2101 ctcactccgg cccaggctga cgggcaccga agagaggcga ctggcgaaac atcacacggc
     2161 gaacgtcgaa gcctaccagc tctacctgag ggggcgttac cacctcctca agaatacgcg
     2221 gtcggagatc cagaccgcta tctcccactt ccggcaggcg gtcgaggtcg acccgtctta
     2281 cgcgctcgcc tacgccgggc tcgcgaacgc gtaccacgcc ctggcaggtc ccggcgagat
     2341 gcccccgaca gaactcctcc cgcaggcgaa ggcggcggcg cagaaggcgg tcgagatcga
     2401 cgacgggctg gccgaggccc acgccctgct cggcttcgtc atcttctggt acgactggga
     2461 ctggggcgcg gccgaggacc agttccggcg cgccctggag ctcgacccga acagcgcgga
     2521 cgcccattac gcctacgcgg atctactcac ctacacgggg cggcacgccg aggcgctcgc
     2581 cgagatcagg cgcgccaggg aactcgaccc gctcaacctg agaatca
//
LOCUS       DBNGOH010000254         5199 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2054020, whole genome shotgun sequence.
ACCESSION   DBNGOH010000254 DBNGOH010000000
VERSION     DBNGOH010000254.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5199)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5199)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5199
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2054020"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(104..721)
                     /locus_tag="AC159P_04800"
     CDS             complement(104..721)
                     /locus_tag="AC159P_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020712532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR3694804.1"
                     /translation="MTNTEVLTAWSVVGREPALAGAEPRKESDYELAQKSAAGDAASF
                     EELYTRHHRRVYGLCLRMMQNVSEAEDLTQEVFVQLFKKIGMFRGDSAFTTWLHRLTV
                     NQVLMHFRRKKNARAEQTSEDGEPPVQIVAGTENPNKMPVVDRIALDRAIAKLPAGYR
                     TVFVLHDVEGFEHDEIARRLGIAVGTSKSQLHKARMKLRGLLKRR"
     gene            complement(868..2094)
                     /locus_tag="AC159P_04805"
     CDS             complement(868..2094)
                     /locus_tag="AC159P_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010537780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3694805.1"
                     /translation="MNTSSIIRIAFRALARNKMRAALTMLGIIIGVASVIAMVSIGQG
                     AQASVQAQIEGMGTNLLFVSAGAQNTGGVRGGAGADGGNNALTAEDLEAIRREVPSVA
                     MVSPQVNSRSQLVAANLNWNTQVQGVSQQYPDIRKWSVESGEFFADTDVRSAARVIVL
                     GRTVADNLFPGTDAVGQTVRVMNLPFRVVGVMARKGQDPQGRDQDDLAFAPYTSVQKK
                     LLGNNRLQVAYVSAISPDATYTAQSQITELLRQRHKLAANEPNDFTVRNMSDVAEAAN
                     ETNNTMTVLLACIAGVSLLVGGIGIMNIMLVSVTERTREIGIRMAIGARSTAVRTQFL
                     IESVVLSLTGGFAGIVLGLALSLAVPLLLGWPTLVSTAAIVGSVVFSAAVGVFFGYYP
                     ARKAAGLDPIEALRYE"
     gene            complement(2218..2919)
                     /locus_tag="AC159P_04810"
     CDS             complement(2218..2919)
                     /locus_tag="AC159P_04810"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013314708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3694806.1"
                     /translation="MGDVEVHALRGVSLTIPEGEFVAIMGTSGSGKSTMMNIVGCLDR
                     PTRGTYVLDGTDVSGLSKDERADIRSRKLGFVFQGFNLLARTSALENVELPMLYAGVE
                     GAERRRRASEALRSVGLAGREQNHPNQLSGGQQQRVAIARALVNDPKLILADEPTGAL
                     DSRTSVEVMEIFQRLNRERGITLVLVTHEADIAAYADRVVVFKDGRITKDYRVREPRD
                     AADELRNLPIVEEEE"
     gene            complement(3208..4944)
                     /locus_tag="AC159P_04815"
     CDS             complement(3208..4944)
                     /locus_tag="AC159P_04815"
                     /inference="COORDINATES: protein motif:HMM:NF052759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR3694807.1"
                     /translation="MEWMKAKLGGKKRIALAFALVVLAASASYWMWGGGASAAEYMTA
                     KAERGNLRNSVTATGALQAVTTVQVGSQASGTISALYVDFNSTVKKGQVIAQLDPSTV
                     QAQVQQARANLQQAQAALQQSRAGLVQSRAGVSQSRAGVTDAQAKAAAAGSTVENQQA
                     GVASAEANVAVLKAQLDDAASVLRQEQSLLQSGVISQREYMTAQTNYKTAEARYNQSV
                     AQLNQARLSQQSAASAGRTQATAQVEQAQSQVQQSQAGVQQSQAQVLNAEAQVQQSQA
                     ALQLAELNMSHTTIRSPIDGVVVSRDVNVGQTVAASLSAPTLFTIAQDLTAMQVIANI
                     DQADIGLVEQAKGVQFNVDAFPADEFKGTIRQIRLNPVSAQNVVTYNVVIDVANPEQK
                     LKPGMTANLTITIDERRDVLKVPNSALRFAPQLTDEQRRSRQEARAGGQGGQNNERRA
                     ARQQQQQQQGAGDAAGNNAGGGDAAAQGEGARRGGGVTPSTSPVLAGQTRRVWVLGAD
                     GQPQPRRIKVGLSDGSATEVVEGELNEGDVIITGQNVAASATRTQSGGGTQSAPGFGG
                     APRGGAGGGRGR"
BASE COUNT          762 a         1832 c         1635 g          970 t
ORIGIN      
        1 gaaagagatc gcttcttctc tgcgccctct gcgtccctcc gcgtcctctg cgttgaaacg
       61 cacgcccggt aaactcaacc gcaatccgcc ctaacggtct tctctaacga cgcttgagca
      121 ggccgcgcag cttcatccgc gccttgtgca gttgcgattt ggaggtgccg accgcgatgc
      181 ccaaccggcg cgcgatctcg tcgtgctcga agccctccac gtcgtgcagg acgaagaccg
      241 tgcggtagcc ggcgggcagt ttggcaatcg cgcggtcgag cgcgatgcgg tcaaccacag
      301 gcatcttgtt cgggttctcc gtcccggcca cgatctgcac gggcggctcg ccgtcttcgg
      361 acgtttgttc ggcgcgcgcg ttcttcttgc ggcggaagtg catcagcacc tggttgacgg
      421 tcaggcggtg gagccacgtc gtgaaggccg agtcgccgcg gaacatgccg atctttttga
      481 agagctggac gaagacctcc tgcgtcaggt cttcggcctc cgagacgttc tgcatcatgc
      541 gcaggcagag gccgtaaacc ctgcggtggt gccgcgtgta gagttcctcg aaagacgcgg
      601 cgtcgcccgc cgccgacttc tgcgccagct cgtaatcgga ttccttacgc ggctcggcac
      661 ccgcgagcgc aggctcgcgc ccgaccaccg accacgccgt taaaacctct gtattcgtca
      721 tatctctttc cggttctaac accctgtacc ttgatagacg ttgcggggcc gccgccggtt
      781 acagacgccg gcggcgggcc gcatgatttc ccgcgttcct tcgaattttt ttacaccact
      841 tttacacccc gcctttttcc gccgccctta ctcgtagcgc agcgcctcga tcgggtccag
      901 tccggcggcc ttgcgcgccg ggtagtagcc gaagaagacg ccgaccgccg ccgagaagac
      961 gaccgagccg acgatggccg cggtcgagac gagcgtcggc cacccgagca ggagcgggac
     1021 ggccaaagag agcgccagac cgagcactat cccggcgaag ccgcccgtca gtgagagaac
     1081 gaccgactcg atcagaaact gcgtcctgac ggcggtcgag cgcgcgccga tggccatacg
     1141 gatgccgatc tcgcgcgtcc gctccgtcac cgagaccagc atgatgttca tgatcccgat
     1201 gccgccgacc agcagcgaga cgcccgcgat gcaggccagc agcaccgtca tcgtgttgtt
     1261 cgtctcgttc gcagcctccg ccacgtcaga catgttacgc accgtgaagt cgttcggttc
     1321 attcgccgcc agcttgtggc gctggcggag cagctccgtg atctgcgact gcgccgtgta
     1381 ggtcgcgtcc ggcgagatgg ccgagacgta ggcgacctgt aggcggttgt tgcccaagag
     1441 cttcttctgc accgaggtgt agggcgcgaa ggccagatcg tcctggtcgc gcccctgcgg
     1501 gtcctgcccc ttgcgcgcca tcacgccgac gacgcggaac ggcagattca tcacgcgcac
     1561 ggtctggccg acggcgtccg tgccggggaa gaggttgtcg gcgacggtgc gcccgagcac
     1621 gatgacgcgc gccgccgagc gcacgtccgt gtcggcgaag aactcgccgc tctcgaccga
     1681 ccacttgcgg atgtccgggt actgctggct gacgccctgc acctgcgtgt tccagttgag
     1741 gttcgcggcg acgagctgcg agcgcgagtt gacctgcggg ctgaccatcg ccacgctcgg
     1801 cacctcgcgc cggatggctt cgaggtcttc cgccgtgagc gcgttgttgc cgccgtccgc
     1861 cccggcgccg ccgcgcacgc cgcccgtgtt ctgcgcgccc gcgctgacga acagcaggtt
     1921 cgtccccatg ccctcgatct gcgcctgcac ggaagcctgc gcgccctgcc cgatgctcac
     1981 catcgcgatc accgacgcga cgccgataat gatgcccagc atggtcagcg ccgcgcgcat
     2041 cttgttgcgc gccagcgccc ggaaggcgat tcgaatgatg ctcgacgtgt tcatggcttt
     2101 actcaactta atagtgctga atgctgagta ctgagtaccg agtaggaaca gcgtacctct
     2161 cgtcaacggt caatgctttc actcagtacc agcactcagt actcagcatt cagcacttta
     2221 ttcttcctct tcgacgatcg gcagattccg cagctcgtcg gcggcgtcgc gcggctctcg
     2281 gacgcggtag tccttcgtga tgcgcccgtc cttgaagacg acgacgcggt cggcgtaagc
     2341 cgcgatgtcc gcctcgtgcg tgacgaggac gagcgtgatg ccgcgctcgc ggttgaggcg
     2401 ctggaagatc tccatcacct cgaccgaggt gcgcgagtcg agcgcgccgg tcggctcgtc
     2461 ggcaaggatg agcttggggt cgttgacgag ggcgcgggcg atggcgacgc gctgttgctg
     2521 gccgcccgag agctggttcg ggtgattctg ctcgcgcccg gcgagtccca ctgaacggag
     2581 cgcctcggac gcgcgccgcc gccgctccgc accctcgaca ccggcgtaga gcatcggcag
     2641 ctccacgttc tccagcgccg acgtgcgcgc cagcaggttg aagccctgaa agacgaagcc
     2701 gagcttgcgg ctacggatgt cggcgcgctc gtccttcgag aggccggaca cgtccgtgcc
     2761 gtccagcacg taggtgccgc gcgtcgggcg gtcgagacag ccgacgatgt tcatcatcgt
     2821 cgatttgccg gagcccgaag tgcccatgat ggcgacgaac tcgccctcgg ggatcgtcag
     2881 cgagacgccg cgcagcgcgt gcacctccac gtcgcccatc gtgtacgtct tgtgaacccc
     2941 ctccaatcgg atgacgggcc gcgcctcttc aacccgcgcg agcagcgccg cgttcacacc
     3001 gaccgccggt cttccgattt caaatgtcgt ctgcatcgct caaccctctc gctgcttcac
     3061 gccgcgccgt gtcgcttcgt tccgcttctt atcaaagtcg tgtcgcttcg cttcgcgtct
     3121 cgcccttcgc ggacgagggc ggcgagccgc cggtgcgttg tcgtcgctcc ggcgattggc
     3181 cgccgtcgtc ttcgaagcgc cacgctgtta cctgcccctg ccgccgcccg cgccgccgcg
     3241 cggtgcgccg ccgaagccgg gcgccgactg cgtgccgccg ccgctctgtg tcctcgtcgc
     3301 ggaggcggcg acgttctggc cggtgatgat gacatcgcct tcgttcagct cgccttcgac
     3361 cacttcggtc gcggagccgt cggagaggcc gaccttgatg cggcgcggct gcggctgtcc
     3421 gtccgcgccg agcacccaca cgcgccgcgt ctggcccgcg agcacgggcg aggttgacgg
     3481 cgtcactccg ccgccgcgcc tcgcgccttc gccctgcgcc gcggcgtcgc cgccgcccgc
     3541 gttgttgccc gcggcgtcgc ccgcgccctg ctgctgctgc tgctgctgtc gcgccgcgcg
     3601 ccgttcgttg ttctgtccgc cctgaccgcc cgcgcgcgct tcttgacgag agcgccgctg
     3661 ttcgtccgtc agttgcggcg cgaagcggag cgccgagttc ggcaccttga gcacgtccct
     3721 gcgctcgtca atcgtgatcg tcaggttcgc cgtcatgccg ggcttcagct tctgctccgg
     3781 gttcgccacg tcgatcacga cgttgtaggt gacgacgttc tgcgcgctca cggggttcag
     3841 acgtatctgc cggatggtgc ccttgaactc gtccgccggg aacgcatcga cgttgaactg
     3901 gacgcccttc gcctgctcga ccagaccgat gtccgcctga tcgatgttgg cgatgacctg
     3961 catcgcggtg aggtcttgcg cgatggtgaa gagcgtcggc gccgaaagcg aggcggccac
     4021 ggtctgcccc acgttcacgt cgcgcgagac caccacgccg tcaatcggcg agcgaatggt
     4081 cgtgtgcgac atgttcagct cggcgagctg gagcgcggcc tgcgactgct gcacttgcgc
     4141 ctcggcgttc aagacttgcg cctgcgactg ctgcacgccg gcctgcgact gctgcacctg
     4201 cgactgcgcc tgctcgacct gcgccgtggc ctgcgtgcga ccggcgctcg ccgccgactg
     4261 ctggctgaga cgcgcctgat tgagctgcgc gacggattgg ttgtagcgtg cctccgcggt
     4321 cttatagttc gtctgtgccg tcatgtactc gcgctgcgag atgacgccgc tctgtaaaag
     4381 cgactgctcc tgacggagga cgctcgcggc gtcgtcgagc tgcgccttga ggacggcgac
     4441 gttggcctcg gcgctggcga ccccggcctg ctggttctcg acggtcgagc cggcggcggc
     4501 ggccttcgcc tgcgcgtccg tcacgcccgc gcgcgactgc gaaacccccg cgcgcgactg
     4561 cacgaggccg gcgcgcgact gctgcaacgc ggcctgcgcc tgctgcaagt tggcccgcgc
     4621 ctgctgcacc tgcgcctgca cggtcgaggg gtcgagctgc gcaatgacct gccccttctt
     4681 gacggtcgag ttgaaatcga cgtagagcgc ggagatcgtc cccgaagcct ggctgccgac
     4741 ctgcaccgtg gtcacggcct gcaacgcgcc ggtcgccgtc acggagttgc gcaggttccc
     4801 gcgctcggcc ttcgccgtca tgtactccgc cgcgctcgcc ccgccgcccc acatccagta
     4861 cgaggcggag gctgccagca ccaccagcgc gaacgccagc gcaatccgct tcttaccgcc
     4921 caactttgcc ttcatccact ccatcgttct ctccccaagc gcgccgttcg gaaacccccg
     4981 ccgcgcttcc ctcaccatgt ctctcagact aaaaatgcgg gcatccaatc gcccacatct
     5041 agacagacac cggcccgcgc cgctcggtta cacccgcggg agattttgga ttttagattt
     5101 cggattttgg attggcgtgc tgcttcggct tgacgccgga tttcggacgt tcaacctgca
     5161 ttacacgaac aatccgaaat ccgaattcta aaatccaaa
//
LOCUS       DBNGOH010000255         2255 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_771103, whole genome shotgun sequence.
ACCESSION   DBNGOH010000255 DBNGOH010000000
VERSION     DBNGOH010000255.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2255)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2255)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2255
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_771103"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..987)
                     /locus_tag="AC159P_04820"
     CDS             complement(<1..987)
                     /locus_tag="AC159P_04820"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3694808.1"
                     /translation="MQHTPTEQQTADQPAPRPVTPKDAAAVILLREDAGDDDAQVFWA
                     RRGAGMAFLPGFYAFPGGQRDERDAEARVEHAGDPEPAAMIACAARELFEETGVLVAR
                     GAEGLTKGQRASVLDDLTSGRMTFAELLRHYGLHLDARDFTFVGRWVTPPFSPRRFDT
                     LFVLVRCPRKQEPRVLTAEFESGEWTRAREALARWRRAEALAVPPVIHALKTLAEGLT
                     EDLVERFLAVPQAHRLSMRRIEFLPGFVCFPVRTPTRPPATTTNSYVVGTRDFVLFDP
                     GSPYEDEQAALREYIDELLAEGRNLRQIILTHLHPDHVAGANALREHLNGRVP"
     gene            complement(1023..1718)
                     /locus_tag="AC159P_04825"
     CDS             complement(1023..1718)
                     /locus_tag="AC159P_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002649027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase-like oxidoreductase"
                     /protein_id="HIR3694809.1"
                     /translation="MGFLDRLIKDPYYQRDDPPRAEEYEGHADVIVSPDTRRERRLPP
                     GQTRTRKWPVLDAHGTPDVDLATWEFEAAGLVERPLKWSLDAFLQLPAVKVFADFHCV
                     TRWSRLGNVWGGVSTREVAKLVGLKPEAKFVSALAYDYGWTTNLPIEYFLAEDSLFAW
                     SHDGQPIPPEHGGPVRLIVPRLYAWKSAKWVKGVRFLAEDRAGFWEEGGYHMRGVPWN
                     GADGERFRYQDDE"
     gene            complement(1828..>2255)
                     /locus_tag="AC159P_04830"
     CDS             complement(1828..>2255)
                     /locus_tag="AC159P_04830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694810.1"
                     /translation="AKEQTTVPDIYRRALTHAREHPEMSLRDLRRRLLDEFEGAPMPP
                     LANLTIPEQDDLAPEEDWTAGLSLIRRGIQNEDWREIVNGILMSLEQTVRYERERGLL
                     GTADEWHDRTAGIKGQTDKGVGKWMPEELMKLAEQATKK"
BASE COUNT          335 a          724 c          804 g          392 t
ORIGIN      
        1 ggggacgcga ccgttcaggt gttcgcggag cgcgttcgcc cccgcgacgt ggtcggggtg
       61 caggtgcgtc aggatgatct gtcggagatt gcggccttcg gccagcagtt cgtcaatgta
      121 ttcgcggagc gcggcctgct cgtcttcgta aggtgagccg ggatcgaaaa ggacgaagtc
      181 acgcgtgccg acgacgtagg agttggtcgt cgtggcgggc ggccttgtag gcgtgcggac
      241 ggggaagcag acgaagccgg gcaggaactc gatgcggcgc atcgagagcc ggtgcgcctg
      301 cgggacggcg aggaagcgct cgaccaaatc ttcggtgagt ccttcggcca gcgttttcag
      361 agcgtggatg acgggcggga cggcgagcgc ttcggcgcgg cgccagcgcg cgagggcttc
      421 gcgcgcgcgc gtccactcgc ccgactcgaa ctcggcggtg agcacgcgtg gctcctgctt
      481 gcgcgggcag cggacaagga cgaagagcgt gtcgaagcgg cgcgggctga acggcggcgt
      541 cacccagcgc ccgacgaagg tgaaatcacg cgcgtcgaga tgaagcccgt agtgccggag
      601 gagttccgcg aacgtcatcc tgcccgatgt caaatcgtcg agcacggagg cgcgctgccc
      661 cttcgtcaag ccttcggccc cgcgcgcgac gagcacgccc gtctcctcga acagctcgcg
      721 cgccgcgcac gcgatcatcg ccgccggctc cgggtcgccc gcgtgctcga cgcgcgcctc
      781 cgcgtcccgt tcgtcgcgct gcccgccggg aaacgcgtag aagcccggca ggaacgccat
      841 ccccgcgccg cgccgcgccc agaagacctg cgcgtcgtca tcgcccgcgt cctcgcgcaa
      901 gaggatgacg gcggcggcgt ccttcggcgt gacgggtcga ggcgccggct ggtctgccgt
      961 ctgttgttcg gttggcgtgt gttgcaagaa gttcttttgg tgttgccgcg ctcggagatt
     1021 ttttattcgt cgtcctggta gcggaagcgc tcgccgtctg cgccgttcca ggggacgccg
     1081 cgcatgtggt agccgccctc ctcccagaag cccgcgcggt cttcggcgag gaagcggacg
     1141 cccttgaccc acttggccga cttccacgcg taaagccgcg ggacaatgag gcggacgggg
     1201 ccgccgtgtt cgggcgggat gggctggccg tcgtgcgacc acgcgaagag cgagtcttcg
     1261 gcgaggaagt attcgatggg gagattcgtc gtccagccgt agtcgtaggc gagcgccgag
     1321 acgaacttcg cttccggctt caggccgact agctttgcca cctcgcgcgt cgagacgccg
     1381 ccccacacgt tgccgagccg cgaccagcgc gtgacgcagt ggaagtcggc gaagaccttc
     1441 accgcaggca gttgcagaaa agcgtcgagc gaccacttca aagggcgctc gaccagaccg
     1501 gcggcctcga actcccacgt cgcgaggtca acgtcgggcg tgccgtgcgc gtcgagcacg
     1561 ggccacttgc gcgtgcgcgt ctggccgggc ggcaggcgcc gctcgcgccg cgtgtccggg
     1621 ctgacgatca cgtccgcgtg cccctcgtac tcctccgccc gcggcgggtc gtcccgctgg
     1681 tagtaagggt ctttgatgag acggtcgaga aaacccataa agcagaagtc ggaagtcgga
     1741 agaaaaactt tcgttagcga atcgtcagcg tgtcggcgtc agcgcccgcg atgcggagcc
     1801 ttctgacctc tgacttctga cttctgacta ctttttagtc gcctgctcgg cgagcttcat
     1861 taattcttcg ggcatccact tgccgacgcc tttgtcggtc tgtcctttga tgccggcggt
     1921 gcggtcgtgc cattcgtcgg cggtgccgag caggccgcgc tcgcgctcgt agcggacggt
     1981 ctgctcgagg ctcatcagga tgccgttgac gatctcgcgc cagtcctcgt tctggatgcc
     2041 gcggcggatg agggagagtc cggccgtcca gtcctcctcc ggcgcgaggt cgtcctgctc
     2101 ggggatggtc aggttcgcga gcggcggcat cggcgcgccc tcgaactcgt cgaggaggcg
     2161 gcggcgcaaa tcgcggagcg acatctccgg gtgctcgcgg gcgtgcgtca gcgcgcggcg
     2221 gtagatgtcg ggcacggtcg tctgttcttt agcca
//
LOCUS       DBNGOH010000256         4279 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4987266, whole genome shotgun sequence.
ACCESSION   DBNGOH010000256 DBNGOH010000000
VERSION     DBNGOH010000256.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4279)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4279)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4279
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4987266"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(119..652)
                     /locus_tag="AC159P_04835"
     CDS             complement(119..652)
                     /locus_tag="AC159P_04835"
                     /EC_number="1.16.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015150547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dps family protein"
                     /protein_id="HIR3694811.1"
                     /translation="MSSNVGAEVRAMPIDIGIEEKDRREIAEGLSRLLADTYTLYLKT
                     HNFHWNVTGPMFQTLHLMFEQQYNELALAVDLIAERIRALGYSAPGSYKEFGKLTSIK
                     EADGVPAAEGMIRQLVEGQETVARTARAVFETAGNANDAPTEDLLTERLQVHEKTAWM
                     LRSLLATEGGSSAARGA"
     gene            complement(655..1131)
                     /locus_tag="AC159P_04840"
     CDS             complement(655..1131)
                     /locus_tag="AC159P_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011143722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HIR3694812.1"
                     /translation="MTTGKRMKIEGRLRGAELKLTPQRFAVLEYLTRVAGHPTAEQIG
                     AALNRRFPQASRATVYNTLNALRDAGLVREVRLEDAVARYESETGPHHHFVCRECGRL
                     EDVPPETFDPPAPRRLAGGHRVETCEVVLRGVCAACAGGAKKAMAKRMANTKRRKG"
     gene            1371..3209
                     /locus_tag="AC159P_04845"
     CDS             1371..3209
                     /locus_tag="AC159P_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014811538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016743 - carboxyl- or
                     carbamoyltransferase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase family protein"
                     /protein_id="HIR3694813.1"
                     /translation="MHILGISAYYHDSAACLVRDGEVVAAAQEERFTRKKHDPNFPAA
                     AVEFCLRRAGITAAEVDYVAFYDKPLLKFERLLETYIDYAPRGLRSFLLAMPVWLKEK
                     LWMRERVAERAGFGGQVLFTEHHESHAASAFFPSPFERAAVLTMDGVGEWATSSYGYG
                     RGSELHLLKELHFPHSLGMLYSAFTYYTGFKVNSGEYKVMGLAPYGEPKYVRLILDEL
                     IDLREDGSLRLNMKYFDFASGLTMTNAKFDRLFGGPPRKSEDELTQREMDLARSVQEV
                     TEEAMLRMARHVHRETGERNLCLAGGVALNCVGNGRVLREGPFERVWVQPAAGDAGGS
                     LGAALSVWYQYLGNARDVGEVCGGHADGMRGSYLGPEFADEEIEEFLLTTGARYRRVA
                     HDEVAGYAARLLAEEKVVGWFQGRMEFGPRALGARSILGDPRSPRMQSQMNLKIKFRE
                     SFRPFAPSVLRERVSEFFELDADSPYMLLVAPVREGLRVPVTEREGRLFGIEKLNVPR
                     STIPAVTHVDYSARVQTVRREDNPRYYDLIHAFGQLTGCPVVVNTSFNVRGEPIVCTP
                     AQGFACFMRTEMDALVLGDFVLLKGEQGEGVFADDAAAWQEEFQLD"
     gene            3202..3630
                     /locus_tag="AC159P_04850"
     CDS             3202..3630
                     /locus_tag="AC159P_04850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694814.1"
                     /translation="MIDPLIRTDDEGQARVTNAQARKTAMVVAAVLALVAAWNLYRGR
                     ATVVAVAGGAALALALAGLLVPSAARRFHVFWMRVAAVLGYVNSRVLLTVLFYGLFAP
                     YGVVSRLLRDPLRRRGARRESYWVERKHTRQAREQFERLF"
     gene            3663..3821
                     /locus_tag="AC159P_04855"
     CDS             3663..3821
                     /locus_tag="AC159P_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5989 family protein"
                     /protein_id="HIR3694815.1"
                     /translation="MGKLSLAGEIWLFLRQNKKLWLLPIIVVLLLMSALLVFAQSSAL
                     APFIYTIF"
     gene            4056..>4279
                     /locus_tag="AC159P_04860"
     CDS             4056..>4279
                     /locus_tag="AC159P_04860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694816.1"
                     /translation="MLEAETGGLLFVVGDEDGARAARGAEGDVCAPEAPLVGRVVAGE
                     PEAITELYAHYAPLVQRILLARVPNGEVGAL"
BASE COUNT          618 a         1385 c         1501 g          775 t
ORIGIN      
        1 gatctaaccc tccgcgtcct ctgcgcttct ccgcgcactc cgcgttataa ccaaccgcgt
       61 gccacactca accgcgcgcc gcgccgagcg ttaccgctgc ttcgatcttc gccgcagttt
      121 acgcgccgcg tgcggcactg ctgccgcctt cggtggcgag gaggctccgc agcatccacg
      181 cggtcttctc gtggacttgc aggcgctcgg tcagcaggtc ttcggtcggc gcgtcgttgg
      241 cattgccggc ggtctcgaag accgcgcggg cggtgcgcgc caccgtctcc tggccctcga
      301 ccagttggcg gatcatcccc tctgcggcgg gcacgccgtc cgcctccttg atcgaagtca
      361 gcttgccgaa ctccttgtac gagccgggcg ccgagtagcc gagcgcgcgg atgcgctcgg
      421 cgatgagatc gacagcgagc gccagctcgt tgtactgctg ctcgaacatc aggtgcagcg
      481 tctggaacat cggccccgtg acgttccagt gaaagttgtg cgtcttcaaa tagagggtgt
      541 aggtgtcggc cagcaggcgc gacagcccct cggcgatctc gcgcctgtcc ttctcctcga
      601 tcccgatgtc gatcggcatc gccctcacct cggccccgac gttgcttgac atgtttaccc
      661 ctttctcctt ttcgtgttcg ccatcctctt cgccatcgcc ttcttcgcgc cgcccgcgca
      721 cgccgcgcac acgccgcgca gcacgacctc gcaggtctcg acgcggtggc cgcccgcgag
      781 ccgccgcggc gcgggcgggt cgaacgtttc gggcggcacg tcttcgagcc tgccgcactc
      841 gcggcagacg aagtggtggt gcggccccgt ctcggactcg tagcgcgcga cggcgtcttc
      901 gagccggact tcgcgcacga ggccggcgtc ccgcagcgcg ttcagcgtgt tgtagacggt
      961 cgcgcgcgac gcctgcggga accggcggtt gagggccgcg ccgatctgct cggcggtcgg
     1021 gtggcccgcg acgcgcgtca ggtattccag cacggcgaag cgctgcggcg tcagcttcaa
     1081 ctccgccccg cgcagccgcc cctcgatctt catcctcttc ccggtcgtca tccctgtgtt
     1141 gtttagatta agtctaaact gagaattttt caagccccgg cggaaggcgg aaagtggaaa
     1201 ggcgcggggg gcaagtgagg ggccgggaaa agccccgtgt ctccactcgc cccccgcgcc
     1261 tttccacttt ccgccgctcc ccgacgggtt gcccctcgcg gctgtagcgg cgataatcca
     1321 caaccttccg cgcccttcga aaggccggtc gcgcgttccc gttaagaagc atgcacatcc
     1381 tcggcatctc cgcttactac cacgactccg ccgcgtgcct cgtgcgcgac ggcgaggtcg
     1441 tggccgccgc gcaggaggag cgcttcacgc gcaaaaagca cgatccgaac ttccctgcgg
     1501 cggcggtcga gttctgcctg cggcgcgcgg ggataacggc tgcggaggtg gactacgtcg
     1561 ccttctacga caagccgctg ctgaagttcg agcggctgct cgaaacctac atcgactacg
     1621 cgccgcgggg cctgcgctcg ttcctgctgg cgatgccggt gtggctgaag gagaagctgt
     1681 ggatgcgcga gcgcgtggcg gagcgcgcgg gcttcggagg gcaggtgctc ttcacggagc
     1741 accacgagtc gcacgccgcg tcggccttct tcccgtcgcc gttcgagcgc gcggcggtct
     1801 tgacgatgga cggcgtcggc gagtgggcga cctcctcgta cggctacggt cgcggcagcg
     1861 agctgcacct cttgaaagag cttcacttcc cgcacagtct ggggatgctc tactcggcct
     1921 tcacttatta cacgggcttc aaggtcaact ccggcgagta caaggtgatg ggcctcgcgc
     1981 cgtacggcga gccgaagtac gtgcggctca tcctcgacga gctgatcgat ctgcgcgagg
     2041 acgggtcttt gcgtctcaac atgaagtact tcgacttcgc gtcggggctg acgatgacga
     2101 acgcgaagtt cgaccggctc ttcggcgggc cgccgcgcaa atcggaagac gagctgacgc
     2161 agcgcgagat ggatttggcg cgctccgtgc aggaggtgac ggaggaggcg atgctgcgga
     2221 tggcgcggca cgttcatcgg gagacgggcg agcggaatct gtgtctcgcg gggggcgtcg
     2281 ctttgaactg cgtcggcaac ggtcgcgtgc tgcgcgaggg gccgttcgag cgcgtgtggg
     2341 ttcagccggc ggcgggcgac gcgggcggca gcctcggcgc ggcgctgtcg gtctggtatc
     2401 agtatctcgg caacgcgcgc gacgttggag aggtgtgcgg cggccacgcg gacgggatgc
     2461 gcggttcgta cctcgggccg gagttcgcgg acgaagagat cgaagagttt ctgctgacga
     2521 cgggcgcgcg ttaccgccgc gtggcgcacg atgaagtcgc cggttacgcg gcgcgccttc
     2581 tggccgaaga gaaggtcgtc ggttggtttc aggggaggat ggagttcggg ccgcgcgctt
     2641 tgggcgcgcg gagcatcctc ggcgacccgc gctcgccgcg catgcagtcg cagatgaatc
     2701 tgaagatcaa gtttcgcgaa tcgttccgcc cgttcgcgcc gtcggtcttg cgcgagcgcg
     2761 tctcggagtt cttcgagctg gacgcggact cgccctacat gctgctggtc gcgcccgtgc
     2821 gcgagggctt gcgcgtcccc gtgacggagc gggaggggcg tttgttcggc atcgagaagc
     2881 tgaacgtgcc gcgctcgacg atcccggcgg tgacgcacgt ggactactcg gcgcgcgtgc
     2941 agaccgtgcg gcgcgaggac aacccgcgct actacgattt gatccacgcg ttcgggcagt
     3001 tgaccggctg cccggtcgtc gtcaacacct ctttcaacgt gcgcggcgag ccgatagtgt
     3061 gcacgcccgc gcaggggttc gcgtgcttca tgcggacgga gatggacgcg ctcgtcctgg
     3121 gcgacttcgt gctgctgaag ggcgagcagg gcgagggcgt cttcgcggac gacgccgcgg
     3181 cgtggcagga ggagttccag cttgattgac ccgctgataa ggacggacga cgaagggcag
     3241 gcgcgcgtga caaacgcgca ggcgcgcaag accgcgatgg tcgtcgccgc cgtgctcgct
     3301 ctcgtcgcgg cgtggaatct ttaccgcggg cgggcgacgg tggtcgcggt tgcgggcggc
     3361 gcggcgctgg cgctcgcgct ggcggggctg ctcgtgccgt cggcggcgcg gcgcttccac
     3421 gtcttctgga tgcgcgtggc ggcggtgctc ggctacgtga acagccgcgt gctgctgacc
     3481 gttctgttct acgggctgtt cgcgccgtac ggcgttgtct cgcgtctgtt gcgcgacccg
     3541 ctgcggcggc gcggcgcgcg gcgcgagagc tactgggtcg agcgtaaaca cacgcggcag
     3601 gcgcgcgagc agttcgagcg gctgttttga ggggcgagca attcgtcggg agagttccga
     3661 ggatgggtaa actttcgctg gccggtgaaa tctggctgtt cctgcggcag aacaagaagc
     3721 tgtggctgct gccgatcatc gtcgtcctgc tcctgatgag cgcgctgctc gtcttcgcgc
     3781 agtcgtcggc gctcgcgccc ttcatctaca cgatcttctg acgccgcctc gccggacgaa
     3841 acacaacggg ccgcgggttc gcgcggataa aacgcggatc agaaatgatt cgatttcgaa
     3901 tccgcgattt atccgcgaac ccgcggcttc tctcctgact cctcgcttcg gctcgcgcga
     3961 gtcgaaaaaa tcctgataac gattgcgcgg gcgcggcgtc ttaactcagc gaaaggggcg
     4021 cgagacgtaa agcgtgcggg cagagttgaa gaccgatgct tgaggccgag acaggcgggc
     4081 tgctcttcgt ggtcggcgac gaggacggcg ctcgcgcggc gcgcggcgcc gagggagacg
     4141 tgtgcgcgcc ggaggccccg ctcgtcggac gggtcgttgc gggcgaaccc gaggccataa
     4201 ccgagttgta cgcccactac gcgccgctgg tgcaacgcat cctgctcgcc cgcgtgccca
     4261 acggcgaggt gggcgccct
//
LOCUS       DBNGOH010000257         3108 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1139019, whole genome shotgun sequence.
ACCESSION   DBNGOH010000257 DBNGOH010000000
VERSION     DBNGOH010000257.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3108)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3108)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3108
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1139019"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            50..439
                     /locus_tag="AC159P_04865"
     CDS             50..439
                     /locus_tag="AC159P_04865"
                     /inference="COORDINATES: protein motif:HMM:NF024143.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HIR3694817.1"
                     /translation="MANKLQRVRDDDADMGTKLTCFAIGAAVGAMVALLFAPKAGREL
                     REDIADATRKGVDRARETGSQLGAKAGEVYDAASARAGELAQTARAEASRRGEQFTAA
                     IEAGKQAYRDEKRRTESSVMLEPSSGE"
     gene            538..1101
                     /locus_tag="AC159P_04870"
     CDS             538..1101
                     /locus_tag="AC159P_04870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694818.1"
                     /translation="MALQMSTNDPAFWVMVVVAVSFVVIAAAMVVVAVVVGRVVRVVT
                     NVERRVEPLMERVSALAEQVRQIAVQGKDVAEQVTLMSGHLSTASMHFAETSALIKDE
                     VRELKQIVGVTAETARDKVEMISRSIDETHGQLVATTSLINAKIVSPAREIAAIMAGV
                     RRGLEVLVAPTPKQIDQTYGEDEMFIG"
     gene            1189..1407
                     /locus_tag="AC159P_04875"
     CDS             1189..1407
                     /locus_tag="AC159P_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007467009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6980 family protein"
                     /protein_id="HIR3694819.1"
                     /translation="MSADNTHCCDEMTRQVNRRCDTHPGPFDCPDNLVHYSARFDEYG
                     LIIHDGGSSSVRVEYCPWCGAKLPESKR"
     gene            1476..>3108
                     /locus_tag="AC159P_04880"
     CDS             1476..>3108
                     /locus_tag="AC159P_04880"
                     /inference="COORDINATES: protein motif:HMM:NF012361.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HIR3694820.1"
                     /translation="MSTETPLELKRTVNLPKTGFAQKANLGQSEPARLKKWAEIDLYA
                     LIRRACEGREKFILHDGPPYANSDIHIGTALNKILKDLVVKSYTMLGYDSPYVPGYDC
                     HGLPIEQFVERGLEKKNKKRSDLSAVSFRRACREHAAEALKRQTRDFQRLGVTGLWED
                     PYLTMSNHYEAETARLFGRFVERGYVYKGARPVYWCIRDQTALAEAEVEYDEHTSPSV
                     YVKFPLRSDPAAIDERLAGRNVFFLIWTTTPWTLPANLGIAVNPLFEYAAFEAGDEVY
                     VVAGDLLDNVVEKCGLGAVRELEATGTGEDFKVKEVMRPNVVARFMGAQLDRLECGHA
                     WLDRPSLVMLGEHVTLGGEADAESELDVKEARDKRATAKAGTGLVHTAPGHGHDDFVI
                     GTRYGLDVYCPVDNAGRFTPEVEHFAGLGVFEANPKIVEFMRERGVLLYSEEYGHRYP
                     HCWRCKQPVIFRATPQWFISLDAVSRTTTTNEFEQDDDGRDRTNFTENETEAPESLRD
                     GALREVARVRWVPAWGEDRMRNMLTNRPDWCVSRQR"
BASE COUNT          578 a         1004 c         1059 g          467 t
ORIGIN      
        1 actcgaaatt gcgttcgacg gggccttgcg ccccggagga gagtgccgca tggcaaacaa
       61 gttgcagcgc gtgcgcgacg acgacgcgga catggggacg aaactgacgt gcttcgccat
      121 cggcgcggcg gtcggcgcga tggtcgcgct ccttttcgcg ccgaaggcgg gccgcgagct
      181 gcgcgaggac atcgccgacg cgacgcgcaa gggcgttgac cgcgcgcgcg agaccggctc
      241 gcagctcggc gcgaaggccg gcgaagtcta cgacgcggcc tcggcgcgcg cgggcgagct
      301 ggcgcagacg gcgcgcgccg aagcctcgcg ccgcggcgag cagttcaccg ccgccatcga
      361 ggccggcaag caggcttacc gcgacgagaa gcgccgcacc gaaagctccg tcatgctgga
      421 gccttcgagc ggcgagtaat tcataagctg ttagctgtta gccgttagct ttcggctaaa
      481 acaaaagcaa tcgctgaaag ctaacggcta acagcaaacg gctaacggct aactcttatg
      541 gccctgcaaa tgagcacgaa cgacccggcc ttctgggtga tggtggtggt cgccgtctcg
      601 ttcgtcgtca tcgcggcggc gatggtcgtc gtcgcggtgg tggtgggccg cgtcgtgcgc
      661 gtggtgacga acgtcgagcg gcgggtcgag ccgctgatgg agcgcgtctc ggcgctcgcc
      721 gagcaggtgc gccagatcgc cgtgcagggc aaggacgtgg ccgagcaggt gacgctcatg
      781 tccgggcatc tttcgaccgc ctcgatgcac ttcgcggaga cctcggcgct catcaaggac
      841 gaggtgcgcg agctgaagca gatcgtcggc gtgacggcgg agacggcgcg cgacaaggtc
      901 gagatgatca gccgctcgat cgacgagacg cacgggcagc tcgtcgccac cacctcgctc
      961 atcaacgcga agatcgtcag cccggcgcgc gagatcgcgg cgattatggc gggggtgcgg
     1021 cgcggtctgg aagtgctcgt ggcccccacg ccaaaacaga tcgaccagac ctacggggag
     1081 gacgagatgt tcatcggctg aacgcctcgg tccgtaacac aggcgcaacg cgcccgaacc
     1141 atcatccccg tcggggaaat ggctcgggcg ttttgtttgg agagaagcat gtcggcagac
     1201 aacacacatt gctgcgacga gatgacgcga caagtcaacc gccgctgcga cacgcacccc
     1261 ggcccgttcg actgcccgga caacttggtt cactactcgg cgcggttcga cgagtacggg
     1321 ctgatcatcc acgacggcgg ttcgtcgtcc gtccgcgtcg aatattgtcc gtggtgcggc
     1381 gcgaagctgc ccgaatcgaa gcgctgatgc gcccgtcttt tgaaatgccg gacgggtgcg
     1441 ttagtatcga caattcacga agagagagat tcgttatgtc aacagagacc ccgctcgaac
     1501 tgaaaagaac ggtcaacctg ccgaagaccg gcttcgcgca gaaggccaac ctcggacagt
     1561 cggaacccgc gcgcctgaag aagtgggcgg agatcgacct gtacgcgctc atccgccgcg
     1621 cgtgcgaggg gcgcgagaag ttcatcctcc acgacgggcc gccgtacgcg aactccgaca
     1681 tccacatcgg cacggcgctg aacaagattc tcaaagacct cgtggtcaag tcgtacacga
     1741 tgctcggcta cgactcgccg tacgtgccgg gctacgactg ccacgggctg cccatcgagc
     1801 agttcgtcga gcgcgggctg gagaagaaga acaagaagcg ctccgacctt tcggcggtga
     1861 gtttccgccg cgcctgccgc gagcacgcgg cggaagcgct gaagcggcag acgcgcgact
     1921 tccagcgcct cggcgtgacg ggcctgtggg aagacccgta cctgacgatg tcgaaccact
     1981 acgaggcgga gacggcgcgg ctcttcggtc gcttcgtcga gcgcggctac gtctacaagg
     2041 gcgcgcgccc cgtctactgg tgcatccgcg accagaccgc gctcgccgag gccgaggtcg
     2101 agtacgacga gcacaccagc cccagcgtct acgtcaagtt ccctctcagg agcgacccgg
     2161 cggcgattga tgagaggctc gctggtcgaa acgtcttctt cctcatctgg acgacgacgc
     2221 cgtggacttt acccgccaac ctcggcatcg cggtcaaccc gctcttcgag tacgcggcct
     2281 tcgaggccgg cgacgaagtc tatgtcgtcg ccggcgacct gctcgacaac gtggtcgaga
     2341 agtgcggcct cggcgccgtg cgcgagcttg aggcgaccgg caccggcgag gatttcaaag
     2401 taaaagaagt catgcgcccg aacgtcgtcg cgcggttcat gggcgcgcag ctcgaccggc
     2461 tcgaatgcgg gcacgcgtgg ctcgaccgcc cgtcactcgt gatgctcggc gagcacgtga
     2521 cgctcggcgg cgaggccgac gccgagagcg agctggatgt gaaggaggcg cgcgacaagc
     2581 gtgcgacggc gaaggccggg acgggtctcg tgcacacggc gccgggccac ggccacgacg
     2641 acttcgtcat cggcacgcgc tacggcctcg acgtttactg cccggtcgat aacgccggtc
     2701 gcttcacccc cgaggtcgag cacttcgccg gactcggcgt cttcgaggcc aacccgaaga
     2761 tcgtcgagtt catgcgcgag cgcggcgtgc tgctctacag cgaggagtac ggccaccgct
     2821 acccgcactg ctggcgctgc aagcagcccg tcatcttccg cgccacgccg caatggttca
     2881 tctcgctcga cgccgtctcg cgcacgacga cgacgaacga gttcgagcag gacgacgacg
     2941 ggcgcgaccg gacgaacttc accgagaacg agacggaggc gccggagtcc ttgcgcgacg
     3001 gcgcgctgcg cgaggtggcg cgcgtccgct gggtgccggc gtggggcgaa gaccggatgc
     3061 ggaacatgct cacgaaccgc cccgactggt gcgtctcgcg ccagcggg
//
LOCUS       DBNGOH010000258         4207 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1688596, whole genome shotgun sequence.
ACCESSION   DBNGOH010000258 DBNGOH010000000
VERSION     DBNGOH010000258.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4207)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4207)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4207
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1688596"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..503
                     /locus_tag="AC159P_04885"
     CDS             <1..503
                     /locus_tag="AC159P_04885"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HIR3694821.1"
                     /translation="AILRSDSGHNTIFTLDVEGVGPSEVLFLDRQLDPVRGRLIHADL
                     RRLVRGQKIEVTVRLELDGEPEGVREGEGILEQLLREVEIRCGTRDIPEAIHVDVSGL
                     GVHEVMHVSDLKVAENVEILTDPETVIATVSVVREEEVEPAPEETTAEPEVIGKGKKE
                     DEEGEG"
     gene            621..1184
                     /gene="pth"
                     /locus_tag="AC159P_04890"
     CDS             621..1184
                     /gene="pth"
                     /locus_tag="AC159P_04890"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - peptidyl-tRNA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HIR3694822.1"
                     /translation="MLIVGLGNPGAEYEWTRHNLGFMVVDLVARQAGAQVKRAECRAL
                     VGRAELEGRAVELVKPQTYMNLSGESVACLLRKRAGLKPATELLVVSDDIALPFGALR
                     LRPRGSSGGQKGLKNIIDALGTDEFMRLRVGIKPEHPVSSTIGYVLERFPRAQRDEVD
                     KVLERATDALRAVIRDGIDKAMAQFNS"
     gene            1321..1731
                     /gene="rpsF"
                     /locus_tag="AC159P_04895"
     CDS             1321..1731
                     /gene="rpsF"
                     /locus_tag="AC159P_04895"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HIR3694823.1"
                     /translation="MAEKRVYEVVFIVDPDTGEEDFTRLTESLQHIVSDQGGTITKTE
                     NMGRRQLAYRIGRKTDGVFMLFEIEGTGSEIAELERRMRVSDQVMRYLTVRVDEDRRR
                     AEKLKARRAHKASKKPFAGGGANASAGGAGDEGA"
     gene            1868..2173
                     /gene="rpsR"
                     /locus_tag="AC159P_04900"
     CDS             1868..2173
                     /gene="rpsR"
                     /locus_tag="AC159P_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HIR3694824.1"
                     /translation="MSRNFNDDRGFDMDDDGPPQRGPGGRQGGRGRFRRRKVCRFCAD
                     KVDLIDFKDVKLLQNFIPERGKVLPRRISGVCATHQRMLAEAIKRARNIALLPYAAD"
     gene            2194..2760
                     /gene="rplI"
                     /locus_tag="AC159P_04905"
     CDS             2194..2760
                     /gene="rplI"
                     /locus_tag="AC159P_04905"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HIR3694825.1"
                     /translation="MATTTVLLREDVDNLGARGEIVKVKAGFARNYLLPRKLAVEATS
                     GNVKQIEAERAALMKKEAKERGGAEAQAEQMKSLRLDFERKVGEHGMLYGSVTSMHIA
                     EALKERGYEIDRRRIHLPEAIKETGEHTVSVRLHRDVTIEIPVNVNPEGGAQAATATE
                     ETAGAESAEPTAEAAEESTEETAEAQEA"
     gene            2954..>4207
                     /gene="dnaB"
                     /locus_tag="AC159P_04910"
     CDS             2954..>4207
                     /gene="dnaB"
                     /locus_tag="AC159P_04910"
                     /inference="COORDINATES: protein motif:HMM:TIGR00665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HIR3694826.1"
                     /translation="MATYPTDTAREQLLERPLPHSADAERAILGAIILDNALVNQAIE
                     LLRPDDFYVRAHQFVFRAMIALSERGSEINPILLGEELRREGVVEQVGGISFISELTY
                     GLPHFTNVAAYAKVVKDASMMRQLVKVANKITSEALEGEDEAVVILDHAEQMIFALAD
                     ERTRQGFSHVKPVADQLLEKVQEMAGRSAMLTGLTTGFSELDSMTSGLQPSDLIIVAA
                     RPSMGKTSLCLTLAQNSALQAGAVVGIFSLEMSKESLVMRMLCSEGRVDAHRFRSGFL
                     SRDEWARLAGALGSLAEAKIFIDDTPGISVLEMRAKARRLAAEQKRLDLIVVDYLQLM
                     SGSSRRSESRQQEVSQISRELKGLAKELNVPLVALSQLSRAAETRTDHRPQLSDLRES
                     GAIEQDADLVAFIYREEQYNRTEENA"
BASE COUNT          806 a         1362 c         1405 g          634 t
ORIGIN      
        1 ccgcgatcct gcgttcggac tcgggccaca acacgatctt cacgctcgac gtggaaggcg
       61 tcggcccgag cgaggtgctg ttcctcgacc gccagctcga ccccgtgcgc ggtcgcctga
      121 ttcacgcaga cctgcgccgc ctggtgcgcg gccagaagat cgaagtgacg gtgcgtctcg
      181 aactcgacgg cgagccggag ggcgtgaggg aaggcgaagg catcctcgaa caactcttgc
      241 gcgaggtcga aatccgctgc ggcacgcgcg acatccccga ggcgattcac gttgacgtgt
      301 cgggcctcgg cgttcacgaa gtgatgcacg tttcggactt gaaggtcgcg gagaacgtcg
      361 aaatcctgac cgaccccgag acggtcatcg ccacggtcag tgtcgtgcgc gaggaggaag
      421 tcgaaccggc acccgaagag acgaccgccg aacccgaagt catcggcaag ggcaagaagg
      481 aagacgaaga gggcgagggc taaggccctc cggggcaaga ggggaaaagg gcaaaaggtt
      541 aaaaggaaaa cccgcgccgc ttctccgccg gtcttccttt tcgccccttc ccctcttaac
      601 cttttcccct cggttttctc atgcttatcg tcggtctcgg caatcccggc gcggagtacg
      661 agtggacgcg ccacaacctc ggcttcatgg tcgtagacct cgtggcgcgg caggcgggcg
      721 cgcaggtcaa gcgggcggag tgcagggcgc tcgtcgggcg ggcggagctt gagggtcgcg
      781 cggtcgagtt ggtcaagccg cagacctaca tgaacctgag cggcgagtcg gtcgcgtgcc
      841 tgctgcgcaa gcgcgcgggg ctgaagccgg cgaccgagct gctcgtcgtc tcggacgaca
      901 tcgcgctccc gttcggcgcc ctgcgtttgc gcccgcgcgg ttcgagcggc ggccagaagg
      961 gcctgaagaa catcatcgac gcgctcggca cggacgagtt catgcgcctg cgcgtcggca
     1021 tcaaaccgga gcatccggtc tcaagcacca tcggctacgt gctcgaacgc ttccccagag
     1081 cgcagcgcga cgaggtcgac aaagttctgg agcgcgcgac ggacgcgctc cgcgccgtca
     1141 tcagagacgg cattgacaaa gcgatggcac agttcaactc gtgaaccgtc aaccgtcaac
     1201 cgtgacaaga cgaaaagcag ttctttctta tcacggttca cggttgacgg ttgacggttc
     1261 tatttcttcc ttgcttcagc cgccagaggc cgaggcttga catccagaag gaggatgatt
     1321 ttggcagaga agagagttta cgaagtggtc ttcatcgttg acccggacac gggcgaggaa
     1381 gacttcacac gcctgacgga gagcctccaa cacatcgtgt cggatcaggg cggcacgatc
     1441 accaagacgg aaaacatggg gcggcggcag ctcgcctacc gcatcggtcg caagaccgac
     1501 ggcgtcttca tgctcttcga gatcgaaggc acgggaagcg agatcgccga gcttgagcgc
     1561 cgcatgcgcg tgagcgatca ggtgatgcgc tacctgacgg tgcgcgtgga cgaggatcgc
     1621 cgccgcgccg agaagctgaa ggcgcgccgc gcgcacaagg cttcgaagaa gccgttcgcc
     1681 ggcggcggcg cgaacgcgtc ggcgggcggc gcgggggacg agggcgcgtg aggcgcgggt
     1741 cgccgaggcg gcccggagtc tccctgaata aagtttcggc gaccgccgcc gcgcgcgagt
     1801 tccttttcgc gagcagcgca cgacgcgtgc atgagtcgcc ttcgagaaga gacggaaaga
     1861 gaaaattatg tccaggaact ttaacgacga ccgcggattc gacatggacg acgacgggcc
     1921 gccacaacgc ggccccggcg gcagacaggg tgggcgcggg cgcttccggc gccgcaaggt
     1981 ttgccgcttc tgcgccgaca aggtcgatct catcgacttc aaggacgtga agctgttgca
     2041 gaacttcatc cccgagcgcg gcaaggttct cccgcgccgc atctcgggcg tctgcgccac
     2101 gcaccagcgc atgctggccg aggcgatcaa gcgcgcgcgc aacatcgcgc tgctcccgta
     2161 cgccgccgac tgagagagga agggaaccag aagatggcaa cgacgacagt tcttttacgc
     2221 gaggacgttg acaacctcgg ggcgcgcggc gagatcgtca aggtcaaggc gggttttgcg
     2281 cgcaactacc tgctgccgcg caagctcgcc gtcgaggcga ccagtggcaa cgtcaagcag
     2341 atcgaggccg agcgcgccgc gctgatgaag aaggaagcga aggagcgcgg cggcgccgaa
     2401 gcgcaggccg agcagatgaa atcgctccgg ctcgacttcg agcgcaaggt cggcgagcac
     2461 ggcatgctct acggctcggt cacctcgatg cacatcgccg aggcgctcaa ggagcgcggc
     2521 tacgagatcg accgccgccg catccacctg cccgaggcga tcaaggagac gggcgagcac
     2581 accgtctccg tccgcctgca ccgcgacgtg acgatcgaaa tccccgtcaa cgtcaacccg
     2641 gagggcggcg cacaggcggc gacggcgacc gaggagacgg cgggcgccga aagcgccgag
     2701 ccgaccgccg aggccgccga agagtccacg gaagagaccg ccgaagcgca ggaagcataa
     2761 aaaacttcag tgaaacgggg cgcgacaaag tgactattgt cgcgccccgt gccttgtagt
     2821 ataagacagg gcggcgcgtc ctgcggcgcg ctcccccggc gataaccggg ccgggggcga
     2881 cacgtggggc gcgccctatt tttgtcttcg cgcccgcctc ccgctcgaca cgtttgcgac
     2941 cgaccgactc ccgatggcga cctaccccac tgacaccgcg cgcgaacaac tgctcgaacg
     3001 cccgctgccg cacagcgccg atgccgagcg cgccatcctc ggcgccatca tcctcgacaa
     3061 cgcactggtc aaccaggcca tcgagctttt gcgccccgac gacttctacg tccgcgcaca
     3121 ccagttcgtc ttccgcgcca tgatcgcctt gagcgagcgc ggctccgaga tcaacccgat
     3181 cctgctcggc gaagaactca ggcgcgaggg cgtggtcgag caggtcggcg gcatctcgtt
     3241 catctccgag ctgacgtacg gactgccgca cttcaccaac gtcgcggcct acgcgaaggt
     3301 tgtcaaggac gcctcgatga tgaggcagct cgtgaaggtc gccaacaaga tcacgagcga
     3361 ggcgctggaa ggcgaggacg aggccgtcgt catcctcgac cacgccgagc agatgatctt
     3421 cgccctcgcc gacgagcgca cgcggcaggg cttctcgcac gtcaagccgg tcgccgacca
     3481 gctcctcgaa aaggtgcagg agatggccgg tcgctcggcc atgctcaccg gcctcaccac
     3541 cggcttcagc gaactcgact cgatgacctc cggcctccag ccttcggact taatcatcgt
     3601 cgccgcgcgc ccttcgatgg ggaagacctc tctgtgcctg acgctcgcgc agaactcggc
     3661 gctgcaagcc ggggcggtgg tgggcatctt ctctctggag atgtcgaagg agtctttggt
     3721 gatgaggatg ctctgttcgg aggggcgcgt ggacgcgcac cgcttccgca gcggcttcct
     3781 gtcgcgcgac gagtgggcgc ggctcgcggg cgcgctcggc tcgctcgccg aggcgaagat
     3841 tttcatcgac gacacgccgg gcatctccgt gctggagatg cgcgccaagg cgcggcggct
     3901 ggcggcggag cagaagcggc tcgacctgat cgtcgtcgat tacctgcaac tgatgtccgg
     3961 ctcgtcgcgg cgctcggagt cgcggcagca ggaggtctcg cagatttcgc gcgagctgaa
     4021 gggcttggcc aaagagttga acgtgccgct cgtcgcgctc tcgcagctct cgcgcgccgc
     4081 cgagacgcgc accgaccacc gcccgcagtt gtcggacctg cgcgagagcg gcgccatcga
     4141 gcaggacgcg gacctcgtcg ccttcatcta ccgcgaggag cagtacaacc gcacggaaga
     4201 gaacgcg
//
LOCUS       DBNGOH010000259         4949 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_405651, whole genome shotgun sequence.
ACCESSION   DBNGOH010000259 DBNGOH010000000
VERSION     DBNGOH010000259.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4949)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4949)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4949
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_405651"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..365
                     /locus_tag="AC159P_04915"
     CDS             <1..365
                     /locus_tag="AC159P_04915"
                     /inference="COORDINATES: protein motif:HMM:NF013584.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HIR3694827.1"
                     /translation="PGDYSEALAERERLRQELLRWMGGTPLLVAPVGAVPAFEHGARK
                     VFAGGEEVGTFRAFGYAQAFNVFDLPAACVPAGKSREGLPVGVQLVGRPGAERDVLAA
                     ARVVEEALGGWLRPPEFL"
     gene            445..1452
                     /locus_tag="AC159P_04920"
     CDS             445..1452
                     /locus_tag="AC159P_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HIR3694828.1"
                     /translation="MEIAFLFILMFLGIAALVVAAKTIKIVPQATVMLIERLGRFSRV
                     AGSGLNIIVPFLDKPRGVYWSSVRPGLTSIDLREQYIDLPPQPVITRDNVTIHVDSVV
                     YWQITDPVKAVYEVADLIGAIVQLTITGMRAVIGDMDLDHTLSQRDQINAKLRIGLDE
                     ATDKWGVKVTRVDVKNINPPEDVRVTMEKQMTAERTRRALVLQAEGDKQAAITRAEGE
                     KQAAVTRAEGEKQSAILQAEGAAQARLRNAEAEAEAIAQVAGAIADHGNPAQYLITQR
                     YIESLRDMSKGNNAKVIFMPVETSAMLSSIGAIKEMIAETGQTTKREEKPPPPRQLPQ
                     G"
     gene            complement(1691..2287)
                     /gene="thpR"
                     /locus_tag="AC159P_04925"
     CDS             complement(1691..2287)
                     /gene="thpR"
                     /locus_tag="AC159P_04925"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - RNA 2',3'-cyclic
                     3'-phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HIR3694829.1"
                     /translation="MTSTPTRLWRMFYAVELPEEFKARFAAHVARLREAVPDVKASWE
                     RPENIHITVKFVGEVEEGRVGALSEAAGRVAAQAAPFDLTVGGTGAFHTQGNPRVLWV
                     GVEDGSGALARVQERLEDECFALGIPRETRPFHPHLTVARLRAAAGAKQLGTLHRATA
                     FESASFRVTELVLMRSELGPGGSRYTAISRHPFERARD"
     gene            complement(2295..3719)
                     /locus_tag="AC159P_04930"
     CDS             complement(2295..3719)
                     /locus_tag="AC159P_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIR3694830.1"
                     /translation="METKPLLVGGEWRVTAQTREVRSPFSGDLVARFSVAPRETVEES
                     IAAAARAAAEMRDLARHRLADSIRLIAEGIRERAEEFARTIATEAGKPIKLARAEVER
                     GIATFTYAADEARRFAGEVIPLDGQATGAGRVGWTERVPRGVVFGITPFNFPLNLVAH
                     KVAPALASRNAIVVKPSPRTPLTSLLLGEVFLASGLPASALQVVPMEVETIEAVLEDE
                     RVAMISFTGSAEIGWRLRAQSGRKAVALELGGNAPVIVDETADLPLAAERTAAAAFAY
                     AGQVCISAQRVIVHEKVAAPFTEMFVERAGRLRTGDPLDEATELPNMIDEAAARRAEG
                     WINEAVEAGARLLRGGHRRGSLLEATVLTNTHSEMRVNSEEVFAPVATIQSSPAFADA
                     LAEANNTRYGLQAGVFTRDMARALQAFRTLEVGGVMINDAPAFRVDNMPYGGVKQSGA
                     GREGVRYAMEEMTEQRLIVIDPQP"
     gene            complement(3825..4346)
                     /locus_tag="AC159P_04935"
     CDS             complement(3825..4346)
                     /locus_tag="AC159P_04935"
                     /inference="COORDINATES: protein motif:HMM:NF025734.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding domain-containing protein"
                     /protein_id="HIR3694831.1"
                     /translation="MQSDIRRTKRRVVKALKIAAIALAALFAAAQFVRPARTNPAVAQ
                     GQSIESHVRMTPEVAGLLERSCEDCHSNRTDWPWYSHVAPASWFVIDHVDHGRSHLNF
                     SEWAKFDDNEADEMLEQICIEAKKGAMPLDSYTLLHRDAKLSPTDVKTLCDWTHEERQ
                     RIAAAQSAARSAR"
     gene            complement(4466..>4949)
                     /locus_tag="AC159P_04940"
     CDS             complement(4466..>4949)
                     /locus_tag="AC159P_04940"
                     /inference="COORDINATES: protein motif:HMM:NF012976.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dioxygenase family protein"
                     /protein_id="HIR3694832.1"
                     /translation="AEAPKVLTWNTVITAKDEPGRPLLVSGRIYGPDGKTPLEGVTLY
                     VYHTDNTGLYPGDGINPAPRLRGWMKTDREGRYEFRTIKPASYPNSSNPEHVHTKASG
                     VGFAEQWLDNFWFEDDPLIPSDMRAKFNGQGAFSPVMAVKRDADGLLRCVRDIRLARA
                     "
BASE COUNT          767 a         1596 c         1740 g          846 t
ORIGIN      
        1 cgcccggcga ttattcggag gctctggcgg agcgcgagcg gctgcgccaa gagttgttga
       61 ggtggatggg cgggacgccg ctcctcgtcg cgccggtcgg cgccgtccct gcgttcgagc
      121 acggcgcgcg caaggtcttc gcgggcggcg aagaggtcgg gactttccgt gccttcggct
      181 acgcgcaggc gttcaacgtc ttcgacctgc cggcggcgtg cgtgcccgcg gggaagagcc
      241 gtgaaggctt gcccgtcggc gtgcagctcg tcgggcgtcc cggcgcggag cgcgacgtgc
      301 tcgcggcggc gcgcgtcgtc gaggaagcgc tcggcggctg gctgcgccct cccgaatttc
      361 tttaaaacga cggcgcgtat ccgttatagt cttcgtgccg cgagttgttg gtagtgcgag
      421 caacaccccc tgacggaggc cgacatggaa attgctttcc tcttcatcct gatgttcctc
      481 gggatcgccg cgctggtcgt cgcggcgaag acgatcaaga tcgtgccgca ggcgaccgtc
      541 atgctcatcg agcgcctcgg gcgcttcagc cgcgtggcag ggagcgggct gaatatcatc
      601 gtgccgttcc tcgacaagcc gcgcggagtc tactggtcga gcgtgcgacc gggcctcacc
      661 tcgattgact tacgcgagca gtacatcgac ttgccgccgc agcccgtcat cacgcgcgac
      721 aacgtgacga ttcacgtcga ttcggtcgtc tactggcaga ttaccgaccc ggtcaaggcc
      781 gtctacgaag tcgcggattt gatcggcgcc atcgtccagc tcacgatcac gggcatgcgc
      841 gccgtcatcg gcgacatgga cttggatcac acgctctcgc agcgcgacca gatcaacgcc
      901 aagctccgca tcgggctgga cgaggcgacc gacaagtggg gcgtcaaggt gacgcgcgtg
      961 gacgtgaaga acatcaaccc gcccgaggac gtgcgcgtca cgatggagaa gcagatgacc
     1021 gccgagcgca cgcgccgcgc gctcgtcctc caggccgagg gcgacaagca ggccgccatc
     1081 acgcgcgccg aaggcgagaa gcaggccgcc gtcacgcgcg ccgaaggcga gaagcagtcg
     1141 gccatcctgc aagccgaagg cgcggcgcag gcccgcctcc gcaacgcaga ggcggaggcc
     1201 gaagccatcg cgcaggtcgc gggcgccatc gccgaccacg gcaacccggc gcagtacctc
     1261 atcacgcagc gctacatcga aagtctcagg gacatgtcga aaggcaacaa cgcgaaggtc
     1321 atcttcatgc cggtcgaaac gtcggctatg ctctccagca tcggcgccat caaggagatg
     1381 atcgccgaga ccggccagac caccaaacgc gaggaaaagc cccccccgcc gcgtcaactg
     1441 ccgcaaggct agcgaaggaa gaagtaggca gtaggcggta ggcagccagg acggaggaag
     1501 cttgagaggt aaagtcccag gcatctccgc tcgtcaagca gtaaagcaag aggcttgaga
     1561 cgtggcgtct caagcctctt cgttcttctg acatctgact cctgatttcc gtcttctgct
     1621 ttgactgcct cctgcctcct gcttacttcg tgtcttggtg gttaaggctt ctttgacttg
     1681 cccagccgta tcagtcgcgc gcgcgctcga aggggtgacg cgaaatcgcc gtgtaccttg
     1741 agccgcccgg cccgagttcg ctccgcatca gcaccaactc cgtgacgcga aacgacgcgg
     1801 actcgaacgc ggtcgcgcgg tgcagcgtgc cgagctgctt cgcgcccgcc gccgcgcgca
     1861 ggcgtgcgac ggtgaggtgt gggtgaaagg ggcgcgtctc gcgcgggatg ccgagggcga
     1921 agcattcgtc ttcgaggcgc tcctgcacgc gcgccagtgc gcccgacccg tcttcgacgc
     1981 cgacccacag cacgcgcggg ttcccctgcg tgtggaaagc gcccgtgccc ccgaccgtta
     2041 aatcgaaagg cgcggcttgc gccgccacgc gcccggcggc ttcggacagc gcgccgacgc
     2101 ggccctcttc gacctcgccg acgaatttca cggtaatgtg aatattctcg ggccgctccc
     2161 aggacgcttt cacgtcgggc acggcttcgc gcaggcgcgc gacgtgagcg gcgaaacgcg
     2221 ccttgaactc ttcggggagt tcgacggcgt agaacatgcg ccagagtcga gtgggtgtgg
     2281 atgtcatgtc ggaatcaggg ctgcgggtcg atgacgatga gccgttgttc ggtcatctct
     2341 tccatcgcgt agcggacgcc ctcgcgcccg gcgccggact gcttgacgcc gccgtagggc
     2401 atgttgtcca cgcgaaaggc gggcgcgtcg ttaatcatca ctccgccgac ttcgagcgtg
     2461 cggaaggctt ggagggcgcg ggccatgtcg cgcgtgaaga cgcctgcctg aaggccgtag
     2521 cgcgtgttgt tggcttcggc gagcgcgtcc gcgaaagcgg ggctggattg gatggtggcg
     2581 acgggcgcga agacctcttc ggagttgacg cgcatctcgc tgtgggtgtt cgtgagcacg
     2641 gtcgcttcga gcagcgagcc gcgccggtgg ccgccgcgga gcagccgcgc gcccgcttcg
     2701 acggcttcgt ttatccagcc ctcggcgcgg cgggccgccg cctcgtcgat catgttgggc
     2761 agctcggtcg cttcgtcgag cgggtcgccc gtgcgcagac gccccgcgcg ttcgacgaac
     2821 atctccgtga agggcgcggc gactttttcg tggacgatga cgcgctgcgc ggagatgcat
     2881 acctggccgg cgtaggcgaa ggccgcggcg gcggtgcgct cggcggctaa cgggaggtcg
     2941 gcggtctcat cgacgatgac gggggcgttg ccgccgagtt cgagcgcgac ggctttgcgg
     3001 cccgactgcg cgcgcagtcg ccagccgatc tcggcgctgc ccgtgaagga gatcatcgcc
     3061 acgcgctcgt cttcgaggac ggcctcaatc gtctcgacct ccatcggcac gacctggagc
     3121 gccgacgcgg gcagccccga agcgaggaag acttcgccga gcagcagaga ggtgagcggc
     3181 gtgcgcgggc tcggcttgac gacgatggcg ttgcggcttg cgagcgcggg cgcgaccttg
     3241 tgtgcgacga ggttgagagg gaagttgaag ggcgtgatgc cgaagacgac gccgcgcggc
     3301 acgcgctccg tccagccgac gcgcccggcg ccggtcgcct gcccgtcgag cgggatgacc
     3361 tcgcccgcga agcgccgcgc ctcgtcggcg gcgtaggtga aggtcgcgat gccgcgctcg
     3421 acctcggcgc gcgcgagctt gatgggcttg cccgcttccg tcgcgatggt gcgcgcgaac
     3481 tcttcggcgc gttcgcggat gccctcggcg atcaggcgaa tcgagtccgc gagccggtgg
     3541 cgcgcgaggt cgcgcatctc ggcggcggcg cgcgcggcgg cggcgataga ctcttcgacc
     3601 gtctcgcgcg gcgcgacgga gaagcgcgcc acgaggtcgc cgctaaaggg cgagcgaacc
     3661 tcgcgcgtct gcgccgtcac gcgccactcg ccgccgacga gcagtggttt cgtttccatc
     3721 acaccctcaa acgtttcggg aggcgcgccg cgccgcctcg ttctctcgcc gcgtagagtc
     3781 ggtttcggtg tgatgatgtt agcacatgta gacgcccgcg tcggtcatcg cgccgaccgc
     3841 gcggcgctct gcgcggcggc gatgcgctgc cgctcttcgt gcgtccagtc gcagagcgtc
     3901 ttcacgtccg tcggcgagag tttggcgtcc ctgtggagca gcgtgtaaga gtcgagcggc
     3961 atcgcgccct tcttggcctc gatgcagatt tgttcgagca tctcgtccgc ctcgttgtcg
     4021 tcgaacttcg cccactcgga gaagttgagg tggctgcgac cgtgatcgac gtggtcgatg
     4081 acgaaccaag aagcgggcgc gacatggctg taccacggcc agtccgtgcg gttcgagtgg
     4141 cagtcctcgc acgagcgttc gagcagcccc gccacctcgg gcgtcatgcg gacgtggctc
     4201 tcgatggact gcccctgcgc gaccgcggga ttcgtgcgcg cggggcggac gaactgcgcc
     4261 gcggcgaaca gcgcggcgag cgcgatggcc gcaatcttca gcgctttgac gacgcgtctc
     4321 ttcgtgcgac gtatatcgga ttgcatctgt aaagtctcct cccctctctt catcgaaccg
     4381 ttttcaggca acactccgcc gcgggcacgc gctttggtta agcgcgtgcc gtggttgccg
     4441 gcgagtgtgc ggaagtcagt tcaggtcagg cgcgtgcaag cctgatgtcg cggacgcagc
     4501 ggagcaggcc gtcggcgtcg cgcttgacgg ccatgacggg cgagaaggcg ccctgaccgt
     4561 tgaacttcgc gcgcatgtcc gaggggatga gcgggtcgtc ttcgaaccag aagttgtcga
     4621 gccactgttc ggcgaagccc acgcccgaag ccttcgtgtg gacgtgttcg gggttgctgc
     4681 tgttggggta ggaggcgggc ttgatggtgc ggaactcgta gcgcccttcg cggtcggtct
     4741 tcatccagcc gcgcaggcgc ggcgcggggt tgatgccgtc gcccggatag aggccggtgt
     4801 tgtccgtgtg gtagacgtag agcgtgacgc cttcgagcgg cgtcttgccg tcggggccgt
     4861 agatgcggcc cgagacgagg agcgggcggc ccggctcgtc cttcgcggtg atgacggtgt
     4921 tccacgtcag aactttcggc gcctcggcg
//
LOCUS       DBNGOH010000260         2279 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_588919, whole genome shotgun sequence.
ACCESSION   DBNGOH010000260 DBNGOH010000000
VERSION     DBNGOH010000260.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2279)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2279)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2279
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_588919"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..747
                     /locus_tag="AC159P_04945"
     CDS             <1..747
                     /locus_tag="AC159P_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014747975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HIR3694833.1"
                     /translation="LSLNIPDFTRYARSQRSQMMGQALGLPLTMTAFAFIGVAVTSAT
                     LIIYGRAIPNPVELMARFDSVAVVLFAMAVLFAAQLTTNMAANVVSPSNDFSNLNPKL
                     ISYVTGGLITAVIGIVMMPWKLMASMGDYIFTWLIGYSGLMGAIAGVMICDYWVLRRR
                     RLDLAGLFKTDGPYSYSSGFNWRAILALVVAIAPVVPGFLRAATTPGGQVAAPGVLDT
                     LYTYAWFVTFALGFVVYYVLMRGHRSLREG"
     gene            793..1023
                     /locus_tag="AC159P_04950"
     CDS             793..1023
                     /locus_tag="AC159P_04950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694834.1"
                     /translation="MRRGLCPKCQTPTVYRKLDGIGYGGSTSGAVFVYTSWITSASHV
                     ENLICTRCGYFETYIVDEKKMAEITNTWEKVG"
     gene            1075..1944
                     /locus_tag="AC159P_04955"
     CDS             1075..1944
                     /locus_tag="AC159P_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020373615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HIR3694835.1"
                     /translation="MPRTVKCGLIQATNAAPTDAPIEEIKRANVEKNVKMIEDAARQG
                     VQILCMQEVFNTPYFCAEQETRWYEAVERIPDGPTVKLMQDVAKQHGMVIVVPIYEEE
                     ITGLYYNTAAVIDADGKYLGKYRKNHIPHVAPGFWEKFYFRPGNLGYPTFETSFARVG
                     VYICYDRHFPEGARALGLNGAEIVFNPSATVAGLSEYLWKLEQPAHAVANGYFVGAIN
                     RVGHEQPWDIGEFYGQSYFCDPRGQIIAEAPRDQDALVVADLDLDMIREVRNTWQFFR
                     DRRPDTYDALVSD"
     gene            1975..>2279
                     /locus_tag="AC159P_04960"
     CDS             1975..>2279
                     /locus_tag="AC159P_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018131100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidine dehydrogenase"
                     /protein_id="HIR3694836.1"
                     /translation="MSSEYQTPLPAEEIERNFREIAPAMTPAEALFEANRCLYCFDAP
                     CTLACPTHIDVPAFIKKIASGNLRGSARVIFDANPVGATCARVCPVEVLCEGACVEKT
                     "
BASE COUNT          409 a          791 c          692 g          387 t
ORIGIN      
        1 ctcagcctga acatccccga cttcacgcgc tacgcgcgct cgcagcgctc gcagatgatg
       61 gggcaggctc tcggcttgcc gctgacgatg acggccttcg ccttcatcgg cgtggcggtg
      121 acgagcgcga cgctcatcat ctacgggcgg gcgattccga accccgtcga gctgatggcg
      181 cgcttcgaca gcgtcgccgt cgtgctcttc gccatggcgg tcctcttcgc cgcgcagctc
      241 acgacgaaca tggccgccaa cgtcgtctcg ccctcgaacg atttctcgaa cctcaacccg
      301 aagctcatct cctacgtcac gggcgggctg atcacggcgg tcatcggcat cgtgatgatg
      361 ccctggaagc tgatggcctc gatgggcgac tacatcttca cgtggctgat cggctactcg
      421 ggactgatgg gcgcgattgc gggcgtcatg atctgcgact attgggtgct gcgccggagg
      481 cggctcgacc tcgccgggct gttcaagacg gacggcccct actcctattc gagcggcttc
      541 aactggcgcg cgattctggc gctcgtcgtc gccatcgcgc cggtcgtgcc cggcttcctg
      601 cgcgcggcca cgacgcccgg cgggcaggtg gccgcgcccg gcgtgctcga cacgctctac
      661 acctacgcgt ggttcgtcac cttcgccctc ggcttcgtcg tctactacgt tttgatgcgc
      721 gggcaccgga gtctgcgcga aggttgaacg cacggcttcc ggcgactcga ccggcgaaag
      781 gagtgacgca acttgagaag aggtctctgc ccgaagtgcc agacgccgac cgtctaccgc
      841 aagctcgacg gcatcggtta cggcggcagc acgagcggcg ccgtgttcgt ctacacttcg
      901 tggataactt cggccagcca cgtcgagaac ttgatctgta cgaggtgcgg ctatttcgag
      961 acgtacatcg ttgacgaaaa gaagatggcc gagattacga acacctggga gaaggtcggc
     1021 tgacaggcgc cgccgccctc caccgccgaa cgataattca gacggaggtc agacatgcca
     1081 cgcacggtta aatgcggact catacaggcg accaacgccg ccccgacgga cgcgcccatc
     1141 gaggagatca agcgcgccaa cgtcgagaag aacgtgaaga tgatcgagga cgccgcgcgt
     1201 cagggcgtgc agattctctg catgcaggag gtcttcaaca cgccttactt ctgcgccgag
     1261 caggagacgc gctggtacga ggccgtcgag cgcatcccgg acggccccac cgtcaagctg
     1321 atgcaggacg tggccaagca gcacggcatg gtcatcgtcg tccccatcta cgaggaggag
     1381 atcaccggcc tctactacaa caccgccgcc gtcatcgacg ccgacggcaa gtacctcggc
     1441 aagtaccgca agaaccacat cccgcacgtc gcgcccggct tctgggagaa gttctacttc
     1501 cgccccggca acctcggcta cccgaccttc gagacttctt tcgcccgcgt cggcgtctac
     1561 atctgctacg accgccactt ccccgaaggc gcgcgcgcgc tgggactgaa cggcgcagag
     1621 atcgtcttca acccctcggc cacggtcgcc ggtctgtccg aatatctgtg gaagctcgaa
     1681 cagcccgcgc acgccgtcgc caacggctac ttcgtcggcg ccatcaaccg cgtcggccac
     1741 gagcagccgt gggacatcgg cgagttttac ggccagagct acttctgcga cccccgcggc
     1801 cagatcatcg ccgaggcccc gcgcgaccaa gacgccctcg tcgtcgccga cctcgacctc
     1861 gacatgatcc gcgaggtcag aaacacgtgg cagttcttcc gcgaccgccg gcccgacacc
     1921 tacgacgcgc tggtctccga ttaactctcg attggcagga agacttgcaa ccttatgtcc
     1981 agcgaatacc agaccccttt gcccgccgag gagatcgagc gcaacttccg cgagatcgcg
     2041 cccgcgatga cgcccgccga agcgctgttc gaggccaacc gctgcctcta ctgcttcgac
     2101 gcgccctgca cgctcgcgtg cccgacgcac atcgacgtgc ccgcgttcat caagaagatc
     2161 gcgagcggca acctgcgcgg gtcggcgcgc gtcatcttcg acgccaaccc cgtgggcgcg
     2221 acgtgcgcgc gcgtgtgccc ggtcgaggtg ctgtgcgagg gcgcgtgcgt ggagaagac
//
LOCUS       DBNGOH010000261         3023 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_405793, whole genome shotgun sequence.
ACCESSION   DBNGOH010000261 DBNGOH010000000
VERSION     DBNGOH010000261.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3023)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3023)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3023
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_405793"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1668
                     /locus_tag="AC159P_04965"
     CDS             <1..1668
                     /locus_tag="AC159P_04965"
                     /inference="COORDINATES: protein motif:HMM:NF012803.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HIR3694837.1"
                     /translation="VVTVVHRLSGWKLLTWLAVSGPPALEIDKLPSASDVHTNIVAGF
                     VSNDGRSVMARLPQAEAELETMAVPALPPGFYGARAARAPLVPSFTLVSGEGKRVNAS
                     FVGFDSATGLSLLEADEPLISFGGDAGHTEEATVGQRVHLFAPAQVGQPGGDEGSTEV
                     EETIYLAMGRTEGELTEVRRAPSGRRARVTARAPRVSPAWAGAVATSESGTPLGIVSL
                     SGAQETQIVPVEVVRAAVERVRATRAGIVPQPWLGVRGYATWQMALDAWVNHGWKPEA
                     ALPLIQSRKGVFLTSIAPGSPAALSGLRNGDVIARTGERDISGVEDLTMLLKEAGVGS
                     TVSFKVWRAGAAAPLDFSAVLSGARSPALATAEAELRAARGRVHEMRAAVTAVRDEER
                     RQRAARIEAERLAAALAELEGRRRQAEHRLTVAQARLAEAEARLGAAHARQARPDGFA
                     LAAAARLMRPLRASGLEVVGLTPRGASQFGARGGVLVVSVLSGSPAERGGLRAGDVIE
                     TAAGKSFTNTELGHLIQNSTEPHLALGVVRGQKKLTVLLPFDGNEEE"
     gene            1882..>3023
                     /locus_tag="AC159P_04970"
     CDS             1882..>3023
                     /locus_tag="AC159P_04970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694838.1"
                     /translation="MGVESNNKEARVAELMLQEAVRHYHQLLEQGDLAESSRQMLDEG
                     LERSKLIFGGRRLSPYLRPHFVTERDWARVCRICETVWGAIQKVKDAAVADESVLDEL
                     GITPIERELVKIDPGYTPVSPTARLDSFLIEDDYSFVELNGESPAGIAYGDAAYEIFA
                     ELPVMRRFAERYNLRRFHGRPLMLRALLDAYAEFLGRQPERTPHIAIVDFKGLPTQKE
                     FELFKEYFEGEGYPSKVCAPEELEFKGGRLCCGDFEIDIVYKRLLVNEYLPVIDTHTA
                     LLDAYRARAVCMVNSFRSKLIHKKALFAVLTNERYAPLFNADEREAIAEHVPWTRKVR
                     EGRTEKDGQTIDLLDFVAAHPDRLVLKPNDDYGGHGIYIGWNHDRVG"
BASE COUNT          473 a         1052 c         1033 g          465 t
ORIGIN      
        1 gtcgtgacgg tcgtgcaccg cctgagcggt tggaaactgc tgacgtggct cgcggtgagc
       61 gggccgcccg cgcttgagat cgacaagctg ccttcggcgt cggacgtgca cacgaacatc
      121 gtggcgggct tcgtctcgaa cgacgggcgc tccgtgatgg cgcgtctgcc gcaggccgag
      181 gccgagcttg agacgatggc ggtgccggcg ctcccgcccg gcttctacgg cgcgcgcgcc
      241 gcgcgcgcgc cgctcgtccc ttcgttcacg ctcgtgagcg gcgaaggcaa gcgcgtcaac
      301 gcgagcttcg tcggcttcga ttcggcgacc ggcctttcgc tgctcgaagc ggacgagccg
      361 ctcatctcct tcggcggcga cgcgggccac acggaagagg cgacggtcgg gcagcgcgtg
      421 cacctgttcg cgcccgcgca ggtcgggcag ccgggcggcg acgaaggctc gaccgaggtc
      481 gaagagacga tctatctcgc catgggccgg acggaaggcg agctgaccga agtccgacgc
      541 gcgccttcgg gtcggcgcgc gagggtgacg gcgcgcgcgc cgcgagtctc gcccgcgtgg
      601 gcgggcgcgg tggcgacgag cgagtcgggc acgccgctcg gcatcgtctc gctgagcggc
      661 gcgcaggaga cgcagatcgt ccccgtcgaa gtcgtgcgcg cggcggtcga gcgcgtgcgc
      721 gcgacgcgcg caggcatcgt cccgcagccg tggctcggcg tgcgcggtta cgcgacttgg
      781 cagatggcgc tcgacgcctg ggtcaaccac ggctggaagc ccgaggctgc gctgccgctg
      841 atccagtcgc gcaaaggcgt cttcctgact tcgattgctc cggggtcgcc cgccgcgctg
      901 tcgggcctgc gcaacggcga cgtgatcgcg cgcacgggcg agcgcgacat cagcggcgtc
      961 gaagacctca cgatgctgct caaggaggcg ggcgtcggct cgaccgtcag cttcaaggtg
     1021 tggcgcgcgg gcgcggcggc cccgctcgac ttctccgcgg tcttgagcgg cgcgcgcagc
     1081 cccgccctcg ccaccgccga ggcggagttg cgggccgcgc gcggcagggt tcacgagatg
     1141 cgcgcggcgg tgaccgccgt gcgggatgaa gagcggcgcc agcgggccgc gcggatcgag
     1201 gccgaacgtc tcgccgcggc gctcgccgaa ctcgaagggc gcaggcggca ggcggagcat
     1261 cggttgacgg tcgcgcaggc ccggctcgcg gaagcggagg cgcgcctcgg cgctgcgcac
     1321 gcgcggcagg cgcggcccga cggcttcgcg ctcgccgccg ccgcgcgtct tatgcgtcca
     1381 ctgcgcgcct ccggtctcga agtcgtcggg ctgacgcccc gcggcgcctc gcagttcggc
     1441 gcgcgcggcg gcgtgctcgt cgtctcggtg ctgtccggca gccccgccga gcgcggcggc
     1501 ttgcgcgcgg gcgacgtgat cgagaccgcc gccggcaaga gcttcaccaa caccgaactc
     1561 ggccacctca tccaaaactc caccgagccg cacctcgcgc tcggcgtcgt ccgcggccag
     1621 aaaaagctca ccgtccttct ccccttcgac gggaacgagg aagagtaggc gaagaagtag
     1681 gcagtaggca gtaggcggta ggcagtcaag acaagagagc gaagaggctc gggaggtaaa
     1741 gtcccaagcc tcttcgctct ttccgacttc tgccttctga cttctgcttt gactgcctac
     1801 tgcctactcc ttgttgcctg ccgcccgctc tctttttcca caccggggcg catctgtgct
     1861 actcttggcg cacataccgt tttgggcgtc gaatcgaata ataaggaagc tcgcgtcgcg
     1921 gaactcatgc tgcaagaggc cgtcaggcat taccatcaat tgcttgagca gggcgacctc
     1981 gccgaatcgt cccggcagat gctcgacgag gggctggagc gctccaagct gatcttcggc
     2041 gggcggcgcc tgtcgcccta cctgcgcccg cacttcgtca ccgagcgtga ctgggcgcgc
     2101 gtctgccgca tctgcgaaac ggtctggggc gcgattcaga aagtcaaaga cgcggcggtc
     2161 gccgacgagt ccgtgctcga cgagctgggc atcaccccga tagagcgcga gctggtgaag
     2221 atcgaccccg gctacacgcc cgtctcgccg accgcgcggc tcgactcgtt cctcatcgaa
     2281 gacgactaca gcttcgtcga gctgaacggc gaaagccccg ccggcatcgc ctacggcgac
     2341 gcggcctacg aaatcttcgc cgagctgccc gtcatgcgtc gcttcgcaga gcgctacaac
     2401 ctgcgccgct ttcacggtcg cccgctcatg cttcgggcgc tcctcgacgc ctacgcggaa
     2461 ttcctgggcc gacagcccga gcgcacaccg cacatcgcca tcgtcgattt caaagggctg
     2521 ccgacgcaga aagagttcga gctgttcaaa gagtatttcg agggcgaggg ctacccctcg
     2581 aaggtctgcg cgcccgagga gcttgagttc aagggggggc gcctgtgctg cggcgacttc
     2641 gagatcgaca tcgtctacaa gcggctcctc gtcaacgagt acctgcccgt catcgacacg
     2701 cacacggcgc tcctcgacgc ctaccgcgcg cgcgccgtct gcatggtcaa cagcttccgc
     2761 tccaagctca tccacaagaa ggcgctcttc gccgtcctga ccaacgagcg ctatgccccg
     2821 ctctttaacg ccgacgagcg cgaagcgatt gcggagcatg taccgtggac gcgcaaggtg
     2881 cgcgaagggc gcacggagaa ggacggccag acgattgacc tgcttgactt cgtcgccgcc
     2941 caccccgacc gcctcgtcct caagcccaac gacgattacg gcggccacgg catctacatc
     3001 ggctggaacc acgaccgcgt cgg
//
LOCUS       DBNGOH010000262         6014 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4254012, whole genome shotgun sequence.
ACCESSION   DBNGOH010000262 DBNGOH010000000
VERSION     DBNGOH010000262.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6014)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6014)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6014
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4254012"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            55..615
                     /locus_tag="AC159P_04975"
     CDS             55..615
                     /locus_tag="AC159P_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011400566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HIR3694839.1"
                     /translation="MEAAVARSQEKVRYDPTYFKIDYPNGDVPAEVGVCTDEVIRSYR
                     KVGVDLQREVHEDMKQNFSVYPKKWGLKKTDTNIDHRRVPNLMVFFERQGAALPVTDR
                     AADYKPGDVVTWDLGGGLTHIGIVVNIPSEGDENRLLIEHNIGAGPQIEDILFNWKIT
                     GHYRYTGPQPKPQPSPTPSRERRRAR"
     gene            641..1720
                     /gene="rsgA"
                     /locus_tag="AC159P_04980"
     CDS             641..1720
                     /gene="rsgA"
                     /locus_tag="AC159P_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007725048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HIR3694840.1"
                     /translation="MEDATNLEDYGWDESFAAHFKLYEREGYEAGRVFLQHNKYYMLY
                     TAAGETLAEASGRLRYEARGPEDFPAVGDWVALRRKEDETKAAVIHDVLPRRSKFSRK
                     AAGTRDEEQVVAANVDTVFLVTGLDHDFNPRRIERYLVMAWESGAEPVVVLNKADVAE
                     DAEGAVREVEAVAPGVAVVRMSAKRGEGVEALLPFVGRGRTVALIGSSGVGKSTIVNR
                     LIGEDVQRTQEVRLGDQRGRHTTTHRELIVLPSGGLVMDTPGMRELQVLVSDRGLRET
                     FDDIEAVAAACRFTDCRHENEPDCAIREALAGGTLDAERYGNYRKLQAEMRQTAARQD
                     QRKGQEEKARVKRLTRAFNKTDKRR"
     gene            1740..2708
                     /locus_tag="AC159P_04985"
     CDS             1740..2708
                     /locus_tag="AC159P_04985"
                     /inference="COORDINATES: protein motif:HMM:NF016424.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HIR3694841.1"
                     /translation="MRTTRIRLAVVISLLFAGAAADAQVAQPTPLPGTYVHDFAGVIA
                     DEREAEIQAKARRLKDEHRTEVAVVTVDSLQGEESFAYSMRMARSWGIGSPEGDVRGL
                     LILVAVKDRKTAFRTSRHVEGELPDGVTGEISRAMNAYFKQGDFGGGLSAGMDKIIAR
                     LKEVGEPQPIAAQQQSPPARGSGIGWLWLPAVIVVPAFGLGYAALRRRGKKRRETAAA
                     AARSPSGFRAWASKVDPTPVPKTKYKSQDVTRAGSRPAKDKKKKSATRSSYQAPSYYE
                     SSGASSSSSSYDSSSSSYDSSSSSSDSGSSYSGGSDFGGGGSDSSW"
     gene            complement(2830..5943)
                     /locus_tag="AC159P_04990"
     CDS             complement(2830..5943)
                     /locus_tag="AC159P_04990"
                     /inference="COORDINATES: protein motif:HMM:NF017044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HIR3694842.1"
                     /translation="MLWKRLLVTLLVTLLCAPGLPALAQKAKGGAKPRAAASKAGAPV
                     LPPLKYTQFFLPNGMRVIFHEDHSTPIVGVNVWYHVGSKNEDKGRTGFAHLFEHMMFQ
                     GFKGYDLDYIPVIQEVGGAINGSTNQDRTNYWELLPSNFLEMALYMEAGRMSGLLEAM
                     TQTKLDNQRDVVKNEKRQRIDNQPYGQVFYKINEAMYPEGHPYRWPVIGSMEDLSAAS
                     MEDVKAFFRRYYVPNNASLVIAGDFDPAEARRLVEKHFGPIARGPEIKRPSPPMPKLD
                     RELREVMEDRVQLPRVQIVWFTSPTYSKDEAALDTLADLLGGGKSSRLYKSLVYEKQI
                     AQSATAFNPTSEISGTFQISVTGKQGTSPAQLEEAIKAELARLKTNPPTAEEVERAYN
                     SREASFVYGLQTVGGFGGKDDKLNGYATYIGKPNHFEQDLARYRAVRAADVARVANQY
                     LTDKRLVMTVVPRGSGQTSAVGPAPQSPRESIAPPAAQTAGQTQAGTTNAASQTERTG
                     TAQGTGAATGQPAGAQTQPKPETAASMPAGAAPPTTAAPGPPRAAASGRPQAPAPKKP
                     AKMVDSSKLPKPGPNPSFTLPPVVRRTLSNGMEVLIVEHKELPIVNMNLVVKMGAAGD
                     PATQAGLASLTADMLDEGTKTRSALDISDQLAFIGSSLSVGSGWDSTTASMTTLTRHL
                     DRALDIYADVVTNPAFADKELARLRDARLTNFKQRRDEPGSIADIVYASQLYGRTHPY
                     GHPLSGDEESIRTIALADVRKFYETFYRPNNSSLIVVGDVTPATLLPKLERAFAKWEK
                     GHIPAVDVAVAPVARDKATLYVVDRPGSAQSVILAGHVGVPRSNPDFFPLLVMNTLLG
                     GQFTSRINMNLREEKGYTYGARSAFDWRRGAGPFFVSAPVQTAVTKESLIEVMKELRG
                     VRGEIPVTAEELEFAKQAIIRGFPRGFETTGQIGGRLEQVVTYGLPDDYFNHYIERVQ
                     TVTLADVNRVAQQHLHPSRMAVVVVGDRKVIEQSLRSLEELGDTVTFLDTEGRPATSG
                     GGSGGTR"
BASE COUNT          954 a         1913 c         2175 g          972 t
ORIGIN      
        1 gcggcgcagc agaagccgag gccgtcgagc ggctcgcctt tcctcgacaa gctcgtggag
       61 gcggcggtcg cgcgctcgca ggagaaggtg cgttacgacc cgacgtactt caagatcgac
      121 tacccgaacg gcgacgtgcc ggcggaggtc ggcgtctgca cggacgaggt catccgctcg
      181 taccgcaagg tcggcgtgga cttgcaaagg gaagtccacg aggacatgaa gcagaacttc
      241 tcggtctacc cgaagaagtg gggcctcaaa aaaacggaca cgaacatcga ccaccgccgc
      301 gtgcccaacc tgatggtctt cttcgagcgg cagggcgcgg cgctgcccgt caccgaccgc
      361 gccgccgact acaagcccgg cgacgtggtg acatgggact taggcggcgg cctgacgcac
      421 atcggcatcg tcgtcaacat cccttccgaa ggcgatgaaa atcgcctcct catcgagcac
      481 aacatcggcg ccgggccgca gatcgaggac atactcttca actggaagat caccggccac
      541 taccgctaca cggggccgca gccgaagcca cagccttcgc cgacaccgtc gcgcgagcga
      601 aggcgcgcgc gatgaatggc ccttcctgat cgagtcatca gtggaagacg caaccaatct
      661 cgaagactac gggtgggacg aatcgttcgc cgcacacttc aagctctacg agcgcgaagg
      721 ctatgaggcc gggcgcgtct tcctgcaaca caacaagtac tacatgctct acacggcggc
      781 gggcgagacg ctggccgagg cttcgggccg tttgcgttac gaggcgcgcg ggccggagga
      841 cttcccggcg gtcggcgact gggtcgcgct gcgccgcaag gaggacgaga cgaaggccgc
      901 cgtcatccac gacgtgctgc cgcggcggag caagttctcg cgcaaggccg ccggcacgcg
      961 cgacgaggag caggtcgtcg ccgccaacgt ggacacggtc ttcctcgtca ccggcctcga
     1021 ccacgacttc aacccgcggc gcatcgagcg ctacctcgtg atggcgtggg agagcggcgc
     1081 cgagccggtc gtcgtcctca acaaggcgga cgtggccgag gacgccgagg gcgcggtgcg
     1141 cgaggtggag gcggtcgcgc ccggcgtcgc cgtcgtgcgg atgagcgcga agcgcggcga
     1201 gggcgtcgaa gcgttgctgc cgttcgtcgg tcgcgggcgc accgtcgcgc tcatcggctc
     1261 ttcgggcgtc ggcaaatcga ccatcgtcaa ccgcctcatc ggcgaggacg tgcagcggac
     1321 gcaggaagta cggctcggcg accagcgcgg gcgacacacc acgacgcacc gcgagctgat
     1381 cgtgctcccg tcgggcggcc tcgtcatgga cacgcccggc atgcgcgagc tgcaagtgct
     1441 ggtgagcgac cgcggcctgc gcgagacgtt cgacgacatc gaagcggtcg ccgccgcgtg
     1501 ccggttcacc gactgccgcc acgagaacga gccggactgc gcgatcaggg aggcgctggc
     1561 gggaggcacg ctcgacgccg agcgctatgg gaactaccgc aagctacagg ccgagatgcg
     1621 ccagaccgcc gcgcggcagg atcagcgcaa agggcaggaa gagaaggcgc gcgtcaaacg
     1681 cctcacgcgc gccttcaaca agacggacaa gcgccgctga ggtgccgcgc cggaggctta
     1741 tgcgaacgac acggattcgg ctggcagtcg tcatctcgct tctcttcgcg ggcgccgcgg
     1801 cggacgcgca ggtggcgcag ccgacgcctt tgccggggac ttacgtccac gacttcgcgg
     1861 gcgtcatcgc ggacgagcgc gaagcggaga tacaggcgaa ggcgcggcgg ctgaaggacg
     1921 aacaccggac cgaggtcgcg gtcgtgaccg ttgattcttt gcagggcgag gagagtttcg
     1981 cgtactcgat gcggatggcc cgctcgtggg gcatcggctc gccggagggc gacgtgcgcg
     2041 gcctgctgat tctcgtcgcg gtcaaggaca ggaagaccgc cttcaggact tcgcgccacg
     2101 tcgagggcga gctgccggac ggcgtgacgg gcgagatcag ccgcgcgatg aacgcctact
     2161 tcaagcaggg cgatttcggc ggcggcctct cggcggggat ggacaaaatc atcgcgcggt
     2221 tgaaagaggt cggcgagccg caaccaatcg cggcgcagca gcagtcgcct cccgcgcgcg
     2281 gttccggaat cggctggttg tggttgcctg ccgtcatcgt cgtccctgcg ttcggcctcg
     2341 gctacgcggc gttgcgccgt cgtgggaaga agaggagaga gacggcggcg gcggcggcgc
     2401 gctcgccttc cggctttcgc gcgtgggcgt cgaaggtaga ccctacgccg gtgccgaaga
     2461 cgaagtacaa gagtcaggac gtgacgagag cggggagccg ccccgctaag gataagaaga
     2521 agaagtctgc aacgcgcagc agctatcaag cgccgagcta ctacgagtcg tccggcgcca
     2581 gctcttcgtc gagcagctac gattcgtctt cgagcagcta cgactcttcg tcgagcagct
     2641 cggactccgg ctcaagctac agcggcggtt ccgacttcgg cggcggcggc tcggactcaa
     2701 gttggtagtg acgcccggtc gataggaagg gggaagaggg gaaaaagttg aaaggcgaaa
     2761 agggaggaaa gcgagccgct ccttctccct tttcgccttt taaccttttc ccctcttccc
     2821 ccccttgtct tacctcgtgc cgccgctgcc gccgcccgag gtggcggggc gcccttcggt
     2881 gtcgaggaag gtgacggtgt cgcccagctc ttcgagcgag cgcagggatt gttcgatgac
     2941 cttgcggtcg ccgacgacca cgaccgccat gcgcgacggg tggaggtgct gttgggcgac
     3001 gcggttaacg tccgcgagcg tgaccgtctg gacgcgctcg atgtagtggt tgaagtagtc
     3061 gtcgggcagg ccgtaggtga cgacctgttc gaggcgcccg ccgatctggc cggtcgtctc
     3121 gaagccgcgc gggaagccgc ggatgatcgc ctgcttggcg aactccagct cctccgccgt
     3181 caccggaatc tcgccgcgca cgccgcgcag ctccttcatc acttcgatca gagactcctt
     3241 cgtcaccgcc gtctgcacgg gcgcgctgac gaagaagggg ccggcgccgc gccgccagtc
     3301 gaaggccgag cgcgcgccgt aggtgtagcc cttctcctcg cgcaggttca tgttgatgcg
     3361 cgaggtgaac tggccgccga gcagcgtgtt catcacgagc agcgggaaga agtccgggtt
     3421 cgagcgcggc acgccgacgt ggcccgcgag gatgaccgac tgcgccgagc cggggcggtc
     3481 aaccacgtag agcgtcgcct tgtcgcgcgc gacgggcgca accgccacgt ccaccgcagg
     3541 gatgtgcccc ttctcccact tggcgaaggc gcgttcgagc ttgggaagta aagtggcggg
     3601 cgtcacgtcg ccgacgacga tgagcgagga gttgttcggg cggtagaagg tctcgtagaa
     3661 cttccgcacg tcggcgagcg cgatggtgcg gatggactcc tcgtcgcccg agagcgggtg
     3721 gccgtacggg tgcgtgcgcc cgtagagctg cgaggcgtag acgatgtcgg cgatgctgcc
     3781 cggctcgtcg cgccgctgct tgaagttcgt caggcgcgcg tcgcgcaggc gcgccagctc
     3841 cttgtcggcg aaggccgggt tggtgacgac atcggcgtag atgtcgagcg cgcggtcgag
     3901 gtggcgcgtc agcgtcgtca tcgaggcggt cgtcgagtcc cagcccgagc cgacggagag
     3961 cgacgagccg atgaaggcga gctggtcgga gatgtccagc gccgagcgcg tcttcgtccc
     4021 ttcgtcgagc atgtcggcgg tcaggctggc gaggccggcc tgcgtcgccg ggtcgcccgc
     4081 cgcgcccatc ttgacgacga ggttcatgtt gacgatgggc agctccttgt gctcgacgat
     4141 gaggacttcc atcccgttcg agagcgtgcg gcgcacgacc ggcgggagcg tgaaggaagg
     4201 gttcggcccc ggcttcggca gcttcgaaga gtcaaccatc ttcgcgggct tcttcggcgc
     4261 aggggcctgc ggcctgccgg acgcggcggc gcggggcggg ccgggcgcgg cggtggtcgg
     4321 cggcgcggcg cccgccggca tcgaggcggc ggtctcgggc ttgggctgcg tctgcgcgcc
     4381 cgcgggctgg ccggtcgcgg cgccggtgcc ttgcgccgtg ccggtgcgct ccgtctgcga
     4441 ggcggcgttc gtggtgccgg cttgcgtctg cccggcggtc tgcgcggcgg gcggcgcgat
     4501 agactcgcgc gggctttgcg gcgcggggcc gaccgcgctc gtctggccgc tgccgcgtgg
     4561 gacgaccgtc atcacgagcc gcttgtccgt gaggtactga ttcgccacgc gggccacgtc
     4621 cgccgcgcgc acggcgcggt agcgcgcgag gtcttgctcg aagtggttcg gcttgccgat
     4681 ataggtcgcg tagccgttga gcttgtcgtc cttgccgccg aagccgccga cggtttgcag
     4741 gccgtagacg aaagacgcct cgcgcgagtt gtaggcgcgc tcgacctcct cggcggtcgg
     4801 cgggttggtc ttgagccggg cgagttcggc cttgatcgcc tcttcgagct gggcggggct
     4861 ggtgccctgc ttacccgtga cggaaatctg gaaggtgccg gagatttcgc tcgtcgggtt
     4921 gaaggccgtg gccgactgcg cgatctgttt ttcgtagacg agcgacttgt agagacgcga
     4981 agacttgccg ccgccgagga ggtcggcgag cgtgtcgagc gccgcctcgt ccttcgagta
     5041 ggtgggcgag gtgaaccaga cgatctggac gcggggaagc tgcacgcggt cttccatcac
     5101 ctcgcgcagc tcgcggtcga gcttcggcat cggcggcgag ggccgcttga tttcggggcc
     5161 gcgtgcgatg gggccgaagt gcttctcgac gaggcgccgc gcctccgccg ggtcgaagtc
     5221 tccggcgatg acgaggctcg cgttgttcgg cacgtagtag cggcggaaga acgccttcac
     5281 gtcctccatc gaggcggccg acaagtcctc catcgacccg atgaccggcc agcggtacgg
     5341 gtgcccctcg gggtacatcg cctcgttgat cttgtagaac acctgcccgt agggctggtt
     5401 gtcgatgcgc tggcgcttct cgttcttgac cacgtcgcgc tggttgtcga gcttggtctg
     5461 cgtcatcgcc tccagcagcc ccgacatgcg cccggcctcc atgtagagcg ccatttcgag
     5521 gaagttcgac ggcagcagct cccagtagtt cgtccgatcc tggttggtcg agccgttgat
     5581 ggcgccgccg acctcctgga tgaccgggat gtagtcgagg tcgtagccct tgaacccctg
     5641 gaacatcatg tgctcgaaga ggtgggcgaa gcccgtccgc cccttgtcct cgttcttcga
     5701 gccgacgtgg taccagacgt tgacgccgac gatgggcgtc gagtggtctt cgtggaagat
     5761 gacacgcatc ccgttgggca ggaagaactg cgtgtacttg agcggcggga gcaccggcgc
     5821 gccggccttc gaggcggcgg cgcgcggctt cgcgccgccc ttcgccttct gcgcgagagc
     5881 cggcagtccg ggcgcgcaga gcagcgtgac gagcagcgtg acgagcagac gtttccagag
     5941 catgtttgac acctccgcga gtgttggcgg cgcggcgctc ggcccgaggg gtcttcgggc
     6001 gcgtactccg gccg
//
LOCUS       DBNGOH010000263         3856 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3704968, whole genome shotgun sequence.
ACCESSION   DBNGOH010000263 DBNGOH010000000
VERSION     DBNGOH010000263.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3856)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3856)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3856
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3704968"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(149..865)
                     /locus_tag="AC159P_04995"
     CDS             complement(149..865)
                     /locus_tag="AC159P_04995"
                     /inference="COORDINATES: protein motif:HMM:NF024895.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HIR3694843.1"
                     /translation="MKLTRTITAALRLVLLLLALTAAARADGGLGLPDPAHPTTAPAR
                     EDCPSSSECPPAVAPDDEATAATPPPPAPRTQPDDEFKHWGLSLHAGVSIPHPDFSDL
                     RRPGPNFGVDLEYRFNKYFSLEGIYTYHRFGGETFGPFVPGGPTFELDPLNLHQLSVN
                     GKIYAAGTSPVRPFFNLGGGAYFISPGSTTRGGVNVGGGLQFDVTETMAVEGAYNFHN
                     VFGFTDVKFSTVQGGVRFRF"
     gene            complement(897..3647)
                     /locus_tag="AC159P_05000"
     CDS             complement(897..3647)
                     /locus_tag="AC159P_05000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694844.1"
                     /translation="MSRKLKEPILDQGIRNVHFFNGRLLTANDLKAEQVAGREQRRHL
                     GSAVGEGVVSGLEVVLESPGSTTADPVVAVTKGLALNRTGHVLSLPGDERVTIARRLE
                     AAPPEAGLFAHCDASSQTFTGLQNGVYLLTLAPASGFAESAPMRGHRPSDTVSGCGSR
                     YAVEGVQFNLTRIDFAALSNLGPDAPAFNPSTLAQVNTLLGLTQDLDRSLLRNVVAHL
                     CFGTEQLTGVAREPFRKVSESAIRERSFYSDYGVADALRKSGAITDCDVPLAVIHWTG
                     GVIRFVDMWSVRRRVYAPAGGRLSHLFTARRISEAEAMFFQFEEHLGALLAALPTPQT
                     LNAANAFRWLPPAGLVPLRNNAFPAGAAHGAFFLGKSFGPPTTINGAKLRTLFDESRH
                     YPPVDLAGTEFVQLYTVRESAQAQGGQNPPQPYVVFATQEMPYYSEQPRFANLCQTLR
                     ETRAAYRDLILRNVFLANATTFGRNLPSPALSLTARLTVTAALQSVMNSASERFTTFC
                     RCGCAISHEKGLAVMQDLYDVQKSLVAVLGTSSANVFSRGLTNVINSGFNNFLSRDTG
                     FGNFIDAGFTNAFDQNTFTFATLLNTYLDTAIPGTAPNNLSLRAAIQAQNLGAAIRAQ
                     NAINGLALNWSGEAVTGSLQVAYRSSDRGLTLVAGDPTPFHYTFRVTNMTNRPLDVQV
                     SAAFDAPSTSWNSGVSLRDTTGAPVSSVTLEPFSQNDPNNPAAFRDVVVAVITPQAAF
                     GSTGTLRFHAEVPPPVGVFDDATPPQPLVIGDAAAPESSTTVTFLAVSFTGIPASAPV
                     GDEITYTFNFRFHTTEEPTTRSFRFFIDIGGAATGFIPYTIRFGNRQSETDPAVVSPT
                     RRATRPFTLTSDADDFVVMHVIPQTGSPSAPLNFTARIQSTDDNELRDETAQQTITAN
                     PN"
BASE COUNT          583 a         1109 c         1529 g          635 t
ORIGIN      
        1 tagctgttag cgattagctg ttagctgtta gctcctggct attggattaa cggttgacga
       61 ttgacgggag cggcgcttgt atcaatcgtc aaccgttaag ctgaaactga aggctaatag
      121 ctaacagcta atcgctaaca gccgaatgtc agaagcggaa ccgcacgccg ccctgcacgg
      181 tcgagaactt cacgtccgtg aagccgaaga cgttgtggaa gttgtaagcg ccttcgacgg
      241 ccatcgtttc cgtcacgtcg aactgaaggc cgccgccgac gttgacgccg ccgcgcgtcg
      301 tggagccggg gctgatgaag taggcgccgc cgccgaggtt gaagaagggg cgcacgggcg
      361 aagtgcccgc cgcgtagatt ttgccgttga ccgagagttg atgaaggttc agcgggtcaa
      421 gctcgaaggt ggggccgccg gggacgaagg ggccgaacgt ctcgccgccg aagcggtggt
      481 aggtgtagat gccttcgagc gagaagtact tgttgaagcg gtattcgaga tcgacgccga
      541 agttcgggcc gggccggcgc aggtcgctga agtccgggtg cgggatgctg acgcccgcgt
      601 gcaggctgag gccccagtgt ttgaattcgt cgtcgggctg cgtgcgcggg gcgggcggcg
      661 gcggcgtcgc cgccgtcgcc tcgtcgtcgg gcgcgacggc gggcgggcac tcggaagaag
      721 aggggcagtc ctcccgcgcg ggcgcggtgg tcgggtgcgc ggggtctggg aggccgaggc
      781 cgccgtcggc gcgcgcggcg gcggtcaagg ccagcagcag gagcacgagc cgcaaggcgg
      841 cggtgatggt gcgtgtgagt ttcaaggtct tctcctctcg tcaatcgtcg gggcgtctag
      901 ttcgggttgg cggtgatggt ctgctgcgcc gtctcgtccc tgagttcgtt gtcgtcggtg
      961 gactggatgc gcgccgtgaa gttgagcggc gcgctcggcg aacccgtctg cgggatgacg
     1021 tgcatgacga cgaagtcgtc ggcgtcgctc gtcagcgtga aggggcgcgt cgcccgccgc
     1081 gtgggcgaga cgacggcggg gtcggtctcg ctctggcggt tgccgaaccg gatggtgtag
     1141 gggataaagc cggttgccgc gccgccgatg tcgatgaaga agcggaagct gcgcgtggtc
     1201 ggctcctcgg tggtgtggaa gcggaagttg aaggtgtagg tgatttcgtc gccgaccggg
     1261 gccgaggcgg ggatgccggt gaaactgacg gcgaggaagg tgacggtggt ggacgactcc
     1321 ggcgccgcgg cgtcgccgat gacgagcggc tgcggcggcg tcgcgtcgtc gaagacgccc
     1381 acgggcggcg gcacctccgc gtggaagcgc agcgtgcccg tcgagccgaa ggcggcctgc
     1441 ggcgtgatga cggcgaccac gacatcgcgg aaggccgccg ggttgttcgg gtcgttctgg
     1501 ctgaaaggct cgagcgtgac ggagctgacg ggggcgccgg tcgtgtcgcg caggctgacg
     1561 cccgagttcc aggacgtgct cggcgcgtcg aaggcggcgc tcacctgcac gtcgagcggg
     1621 cggttggtca tgttcgtgac gcggaaggtg tagtggaagg gcgtcgggtc gccggccacg
     1681 agcgtcagcc cgcggtcgct gctgcggtaa gcgacttgca ggctgccggt cacggcctcg
     1741 cccgaccagt tgagcgcgag gccgttgatg gcgttctgcg cccggatggc ggcgccgagg
     1801 ttctgcgcct ggatggcggc cctgagcgac aggttgttgg gcgcggtgcc ggggatggcc
     1861 gtgtcgagat aggtgttgag caacgtggcg aaggtgaacg tgttctggtc gaaggcgttg
     1921 gtgaagcccg cgtcgatgaa gttgccgaag cccgtgtccc tgctgaggaa gttgttgaag
     1981 cccgagttga tgacgttggt caagcccctg ctgaagacgt tggcggagct tgtgccgagc
     2041 acggcgacga gactcttctg cacgtcgtag aggtcttgca tcacggcgag gcccttctcg
     2101 tgcgaaatgg cgcagccgca gcggcagaag gtggtgaagc gttcgctcgc cgagttcatc
     2161 acggattgca gcgccgccgt caccgtgagg cgcgccgtca gcgacagcgc gggcgacggc
     2221 aggttccgcc cgaaggtggt cgcgttggcg aggaagacgt tccggaggat gaggtcgcgg
     2281 taggcggcgc gcgtctcgcg cagggtctgg cagaggttgg cgaagcgcgg ctgctcggag
     2341 tagtagggca tctcctgcgt ggcgaagacg acgtagggct gcggcgggtt ctgcccgccc
     2401 tgcgcctgcg cgctctcgcg gacggtgtag agctggacga actccgtgcc cgcgagatcg
     2461 acgggcgggt agtggcgcga ctcgtcgaag agcgtgcgca gcttcgcgcc gttgatggtg
     2521 gtcggggggc cgaagctctt gccgaggaag aaggcgccgt gcgcggcgcc ggcggggaag
     2581 gcgttattgc gcagcggcac cagcccggcg gggggcagcc agcggaaggc gttcgcggcg
     2641 ttcaaggttt ggggagtcgg cagcgcagcg agcagcgcgc cgaggtgctc ttcgaactgg
     2701 aagaacatgg cctcggcctc cgagatgcgg cgcgcggtga agaggtgcga aagacgcccc
     2761 ccggcgggcg cgtagacgcg gcggcggaca gaccacatgt ccacgaagcg gatgacgccg
     2821 cccgtccagt ggatgacggc gagcggcacg tcgcagtcgg tgatggcgcc cgacttgcgc
     2881 agcgcgtcgg cgacgccgta gtcgctgtag aagctccgct ccctgatcgc gctctcggag
     2941 accttgcgga aaggctcgcg cgcgacgccc gtcaactgct cggtgccgaa gcagaggtgc
     3001 gccacgacgt tccgcagcag cgagcggtcg aggtcttgcg tgaggccgag cagcgtgttg
     3061 acctgcgcga gcgtcgaggg gttgaaggcg ggcgcgtcgg ggccgaggtt cgagagcgcg
     3121 gcgaagtcga tccgcgtgag gttgaactgg acgccctcga cggcgtagcg gctgccgcag
     3181 cccgagaccg tgtccgaggg gcggtggccg cgcatcggcg cggactcggc gaagcccgag
     3241 gcgggcgcga gcgtgagcag gtagacaccg ttctggaggc cggtgaaggt ctgcgacgag
     3301 gcgtcgcagt gggcgaacag gccggcctcg ggcggcgcgg cttcgagcct gcgggcgatg
     3361 gtgacgcgct cgtcgccggg cagcgagagc acatggcccg tgcggttgag cgcgagtccc
     3421 ttcgtgacgg ccacgaccgg gtcggcggtt gtcgaacccg gcgattcgag cacgacttcg
     3481 aggccggaga cgacgccctc gccgacggcg ctgccgaggt ggcggcgctg ctctcggccc
     3541 gcgacctgtt ccgccttcaa gtcgttcgcg gtcagaaggc gaccgttgaa aaagtggacg
     3601 ttgcgaatcc cctgatcgag gatcggctct ttcagttttc tgctcatggc gaaaagcctt
     3661 cctgcaaact gtgtctctct cgcgcccccg gtcaggccgc ttcgcgcgcg gcggcgaccg
     3721 ctgttgctgc tgctgctgct ccgtcgtcgg aagtcgcggc ggacggcgcg cgcgcgccga
     3781 gcgcttcggt gaagaggagg aagttctgcc ggtgccacgc gtcggcgggg cgcacttcga
     3841 gcgcgcgctt cgtcca
//
LOCUS       DBNGOH010000264         7762 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1688891, whole genome shotgun sequence.
ACCESSION   DBNGOH010000264 DBNGOH010000000
VERSION     DBNGOH010000264.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7762)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7762)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7762
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1688891"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="AC159P_05005"
     CDS             <1..204
                     /locus_tag="AC159P_05005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694845.1"
                     /translation="PGDYEFYAAAFFLVFQANATPPGPRGFNGPRQRVSVAQDGDHNV
                     TLVLDVGEGNTPNVRPVPAPNVP"
     gene            216..2159
                     /locus_tag="AC159P_05010"
     CDS             216..2159
                     /locus_tag="AC159P_05010"
                     /inference="COORDINATES: protein motif:HMM:NF025006.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HIR3694846.1"
                     /translation="MSTSTSSNIAALVFALLFALVFALSPTARAQESPAAASQADPTP
                     TPAATDGPAAKATKGVAGSITGRVLGDGGEPVAGARVVALTSSTSRGPGEPTATTDAS
                     GSFKLDNLAPGLYTINAVLRGHVPDPNEYPSGGPRTLHRPGDSVTIRLVKGGVVTGTV
                     TDAGGEPLVAMSVRAFMVRDLEGDTSTAGYSLGEDQTDDRGVYRIYGLRQGVYVVVAG
                     VATGRPSRGPASVTPFEYDAPTFYPSGTRDTATEVTVRAGQDTAGIDIRHRGERGRRI
                     TGTVTAAQTGSRFETGAIVVLTHAATGTQLSFTPVQPGEGERSFSFEAVADGEYEVQA
                     RLGDGGQFLGASDSRRVVVSGADATGVRLALAPLASLDGSLVVELAGEAARAQAECKG
                     RGANMLPQEAVVTALSEDLSKRNQGARRATLRRETVPDSSGSFTLRGLEAGRYRLDLR
                     LADDNWYVREVQMPAAAPPPQRTPTAATPARPGAANNQNAAGQNAPTAATAPRETVEL
                     KSGQQLAGLTVRVAEGAASLAGRVVPHEENAPLPPLSQLRVHLVPAAREHADNTLRHY
                     ETTLAPDGAFAFRNLAPGRYLLLARPADAAAPDTPPRPAAWDAAARARLRRDAEAADL
                     PVELEPCRRTTDFALRFRQTAGK"
     gene            2363..4042
                     /locus_tag="AC159P_05015"
     CDS             2363..4042
                     /locus_tag="AC159P_05015"
                     /inference="COORDINATES: protein motif:HMM:NF025006.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MSCRAMM family protein"
                     /protein_id="HIR3694847.1"
                     /translation="MRQGIKPDPRRAALAALFLLCCAAPVPARQMGATAAKDAPASVA
                     GRVTREEDNKSAPGVAVTLTASDPATRFKVVARATTDAEGRYRLANVPPGRYQLAPMA
                     PAYVVKDSSWSGWPPGRPVTVAPGENMEDVDFRLARGGVITGRVTDADGKPIVAERVE
                     ITPVDKQQVTGTNWFSGMRNLTDDRGVYRLYGLAAGRYHVSIGQDKDTGVVHIGGPNR
                     YYRRTYHPAATDVAQAQVVEVKPGDEAKDVDIVAASAVKTFKAAGRIVSAETGQPVPD
                     VMFAHGALRKGEKMIGGYGMTGVRTNARGEFTVEGLAPGHYAVFAISHEPADWYSDAV
                     AFEVEEADVSGLEVKLRRGASLGGVVLLEGVSDPAARAQLLLQAQLYASAGSRDELTA
                     PNYLGGKINPDGTFRFGGLRPGQYKIALSTWRAPQGLSLVRVEQNGVSVSGGIEVAEG
                     AQVTGVRVHLAYGTSVVRGQVTTAAGAPAPPPGARMTVHARRVIGGHAEATQARSTEV
                     DVRGRFIIEGLSAGEYLIVLRVTAPGAEAIDVRQTVTVSDGGETSVVLVLK"
     gene            4129..6099
                     /locus_tag="AC159P_05020"
     CDS             4129..6099
                     /locus_tag="AC159P_05020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MSCRAMM family protein"
                     /protein_id="HIR3694848.1"
                     /translation="MTHSPRRPAAPPPRPAVRARSLPSAAAPRRALAALFCATLLACA
                     LGPSRARAQTPAPAQTTRTPDAATETAQDKTGDAARRAPTGSIKGRVLGEDGQPLAHV
                     RLYIHSRSGAAAARQLPPAESDEDGKFVFNELEPGLYGIRPYLPGYTAEAEPSENPSG
                     ANHRLGDDATIRLVRGGVITGTVTRADGEPVVGANVRVNRLRTLDNRPAPALDLWFGN
                     EYATDDRGVYRIYGLPAGVYVVAAGGGQRWSWMPPTPFDADAPTYFRSGTLDTAEEVT
                     VRAGQEVGAVDLRYRGERGYTVSGTVVLPPARKPEDSFNLNISLVHAATGTPLVNTMS
                     QSRDGANAFSFDGIADGEYELRASHYTMGGEGNAAEPLRVSVRGADVPGLKLMPAPLG
                     AIGGDLALEPLREAEGACKAAARRGLIPQEVLVAALREEREGAAEQRRPATPRRRESA
                     PDEGGAFLIRTLEPGRYRLDARPADELWYVRAIQPPAAAPAGTPPAAAPSSTTRPPAS
                     RRDSAAPGAALDLKPGQQFKGLRVVVAEGAASLSGRVAPAADDAPPLPPAARLRVHLV
                     PAQREQADDALRYYETTPAPDGAFAFRHLAPGRYLVVVREAEADAPARPAGWDGAARA
                     RLRREAEAANLSVELEPCRRTTDYALRFNATR"
     gene            6319..7071
                     /locus_tag="AC159P_05025"
     CDS             6319..7071
                     /locus_tag="AC159P_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HIR3694849.1"
                     /translation="MDWLTDPQAWIGLLTLTVLEIVLGIDNVIFISILAGKLPAEQQS
                     KARTIGLALAMLTRILLLFSISLIMRLTTPLFEVFDHGVSGRDLILLVGGLFLIGKST
                     HEIHSKLEGEEGHASARVAPTFASVIIQILLLDIVFSLDSVITAVGMVSNLWVMVTAV
                     VIAVGFMLVFAGPVSAFVERHPTVKMLALSFLLLIGTTLIADGLGFHIPKGYVYTAMA
                     FSVFVEILNLKMRKRKAAKPVKLHEAYTQTTD"
     gene            7171..7728
                     /locus_tag="AC159P_05030"
     CDS             7171..7728
                     /locus_tag="AC159P_05030"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HIR3694850.1"
                     /translation="MRHENTLDLGSTALAVIDMQESFRQAIPDFTELAVRVARVAHAA
                     RLLGVPVLVTEQYPKGLGHTAAEIRAVLPEDFEPMEKTAFSSCGAGAFVAALERAGAR
                     HVLVCGIEAHVCVNQTVHDLLAGGYKVHLLTDCISSRAEHNRHVGLSKMRQSGALPSS
                     TELALFELMRDARHEQFKAIQKLIK"
BASE COUNT         1165 a         3010 c         2569 g         1018 t
ORIGIN      
        1 cccggcgact acgagtttta cgccgccgcg ttcttcctcg tctttcaagc gaacgcgacg
       61 ccccccggcc cgcgcggctt caacggcccg cggcaacgcg tctcggtcgc gcaggacggc
      121 gaccacaacg tcacgctcgt cctcgacgtg ggcgaaggca acacgccgaa cgtccgcccc
      181 gtccctgccc cgaacgtccc ttaaggcaga aaaacgtgag cacctcgacc tcgtccaaca
      241 tcgccgcgct cgtcttcgcg ctcctcttcg cgctcgtctt cgcgctctcg ccgacggcgc
      301 gcgcgcagga gtcgcccgcc gccgcgtctc aggccgatcc cacgccgacg cccgcggcga
      361 ccgacggccc cgccgcgaaa gcgacgaagg gcgtcgccgg ctcgatcacg gggcgcgtgc
      421 tcggcgacgg cggcgagcct gtggcgggcg cccgcgtcgt cgcgctcacc tcctccacga
      481 gccgcggccc cggcgagccg acggcgacga ccgacgcgag cggcagcttc aagctcgaca
      541 acctcgcgcc cggcctctac accatcaacg ccgtcttgcg cggccacgtc cccgacccga
      601 acgaataccc ctcgggcggc ccgaggacgc tccaccggcc cggcgacagc gtgaccatcc
      661 gcctcgtcaa aggcggcgtc gtcaccggca ccgtgacgga cgccggcggc gagccgctcg
      721 tcgccatgag tgtccgcgcc ttcatggtgc gcgacctcga aggcgacacc tcgaccgcgg
      781 gctactcgct cggcgaagac cagacggacg accgcggcgt ctaccgcatc tacggcctcc
      841 ggcagggcgt ctacgtcgtc gtcgcagggg tcgccaccgg tcgcccttcg cgcggccccg
      901 cgtccgtcac gcctttcgag tacgacgcgc cgaccttcta cccttcgggc acgcgcgaca
      961 ccgcgaccga agtcaccgtg cgcgccgggc aggacacggc gggcatcgac atccgccacc
     1021 gcggcgagcg cgggcggcgc atcaccggca ccgtcactgc ggcgcagacg gggtcgcgct
     1081 tcgagaccgg cgccatcgtc gtgctgacgc acgcggccac gggcacgcag ctcagcttca
     1141 cgcccgtcca gccgggcgag ggcgaacggt ctttctcttt cgaggccgtc gccgacggcg
     1201 agtacgaagt gcaggcgcgc ctcggcgacg gcggacaatt cctcggcgcg tcggactcgc
     1261 ggcgcgtcgt cgtcagcggc gcggacgcca ccggcgtccg cctcgcgctc gcgccgctcg
     1321 cctcgctcga cggttcgctc gtcgtcgagc tggcgggcga ggccgcgcgc gcgcaggcgg
     1381 aatgcaaggg gcgcggtgcg aacatgctgc cgcaggaggc cgtcgtcacc gcgctgagcg
     1441 aagacctctc gaagcgtaat cagggggcgc gacgcgccac cctccggcgc gagaccgtgc
     1501 cggactcctc cggctccttc accctgcgcg gcctcgaagc cgggcgctac cgcctcgacc
     1561 tgcgactcgc ggacgacaac tggtacgtcc gcgaagtgca gatgcccgcc gccgccccgc
     1621 cgccgcagag aacgccgacg gcagcgacac ccgcgagacc cggcgcggcc aacaaccaaa
     1681 acgccgccgg gcagaacgca ccgacagcgg ccacggcccc gcgcgagacg gtcgaactca
     1741 agtccggcca gcagctcgcg ggcctcaccg tccgcgtcgc ggagggcgcc gcctcgctcg
     1801 ccggtcgcgt cgtgccgcac gaagagaacg cgccgctccc gccgctctcg caactgcgcg
     1861 tccacctcgt cccggcggcg cgcgagcacg ccgacaacac cctgcgccac tacgagacca
     1921 ccctcgcgcc cgacggcgcc ttcgccttca ggaacctcgc gcccggtcgc tacctgctcc
     1981 tcgcgcgccc cgccgacgcc gccgcgcccg acacgccccc gcgccccgcc gcgtgggacg
     2041 ccgccgcgcg cgcacgcctg cgccgcgacg cagaggccgc cgacctcccc gtcgagcttg
     2101 agccgtgccg gcgcacgacc gacttcgcgc tccgcttccg gcagacggcg ggcaagtaag
     2161 ggcgtcgagc aaagcgagcg gcggcgcatc gaccggctga tgtttggccg gtcggctcga
     2221 acgaaagacc gaacgcggtt cggtcggtca actcgaacgc ggcgcagact cgttcgcgga
     2281 ttgtttgtcg aacggcgtcc ggacgcagac gctctgcggc ccgccgtcga tgtccgacac
     2341 gtacttccga cggagtttcc tcatgcgaca aggaatcaag cccgacccga ggcgcgccgc
     2401 gctcgcggcg ctcttcctgc tctgctgcgc cgcgcccgtc cccgcgcgcc agatgggcgc
     2461 gacggcggcg aaggacgcgc ccgcgtcggt cgcgggccgc gtcacgcgcg aggaggacaa
     2521 caagagcgcg cccggcgtgg ccgtcacgct caccgcgtca gacccggcga cgagattcaa
     2581 ggtcgtggcg cgtgcgacga ccgacgccga gggtcgttac cgtctggcga acgtgccgcc
     2641 gggccgctac cagctcgcgc cgatggcgcc ggcctatgtc gtgaaggatt cgagctggtc
     2701 gggctggccg ccgggccggc ccgtcacggt cgcgccgggc gagaacatgg aggacgtgga
     2761 cttccgcctc gcgcgcggcg gcgtcatcac gggccgcgtc acggacgccg acggcaagcc
     2821 gatcgtcgcc gaacgcgtcg agatcacgcc ggtggacaag cagcaggtga cgggcaccaa
     2881 ctggttcagc ggcatgcgga acctgacgga cgaccgcggc gtctaccgcc tctacggcct
     2941 cgccgccggg cgctaccacg tcagcatcgg gcaggacaaa gataccggcg tggtgcacat
     3001 cggcgggccg aacaggtact accggcgcac gtaccacccg gcggcgaccg acgtggcgca
     3061 ggcgcaggtc gtcgaggtca agccgggcga cgaggcgaag gacgtggaca tcgtcgccgc
     3121 gtccgcggtg aagaccttca aggcggcggg ccgcatcgtc agcgccgaga cggggcagcc
     3181 cgtgcccgac gtgatgttcg cgcacggcgc gctgcggaag ggcgagaaga tgatcggcgg
     3241 ctacggcatg acgggcgtgc ggacgaacgc gcgcggcgag ttcacggtcg aggggctggc
     3301 gccgggccac tacgccgtct tcgccatctc gcacgaaccc gccgactggt acagcgacgc
     3361 cgtcgccttc gaggtcgaag aggccgacgt gagcggactc gaagtcaagc tgcggcgcgg
     3421 cgcgtcgctc ggcggcgtcg tcctgctcga aggcgtgagc gacccggcgg cgcgcgcgca
     3481 acttctgtta caggcgcagc tatatgcttc cgccgggtcg cgcgacgagc tgacggcgcc
     3541 caactatttg ggcggcaaga tcaaccccga cggcacgttc cgcttcggcg gcctgcgccc
     3601 cggccagtat aagatcgcgc tctccacgtg gcgggcgccg cagggtctgt cgctcgtgcg
     3661 cgtcgagcag aacggcgtct cggtgagcgg cggcatcgag gtggcggaag gcgcgcaggt
     3721 gacgggcgtg cgcgtccacc tcgcctacgg cacgagcgtc gtgcgcgggc aggtcaccac
     3781 ggcggcgggc gcgcccgcgc cgccgccggg cgcgcggatg acggttcacg cgcggcgcgt
     3841 gatcggcggc cacgccgagg ccacacaggc gcgctccacc gaggtggacg tgcgcggtcg
     3901 cttcatcatc gaagggctga gcgcgggcga atacctgatc gtgctgcgcg tcacggcgcc
     3961 cggcgccgag gcgatcgatg tcaggcagac cgtcaccgtc tccgacggcg gcgaaacgtc
     4021 cgtcgtcctc gtcctcaaat gagccgcggg cgccgccgcc tccgggcgac gcgcgccggc
     4081 gcctccggcc cctcaagcgc gccgacgcct gacgaaagtg acaccgccat gacccactcg
     4141 cctcgccgac ccgccgcgcc gccgccgcgc cccgctgtac gcgcgcgttc tctgccctcc
     4201 gccgccgcgc cgcgccgcgc gctcgccgcg ctcttctgcg cgaccttgct cgcgtgcgcg
     4261 ctcggcccgt cccgcgcgcg cgcgcagacg cccgcgcccg cgcagacgac gcggacgccg
     4321 gacgccgcca ccgagacggc tcaggacaag acgggcgacg ccgcgcgccg cgctccgacc
     4381 ggctcgatca aggggcgcgt cctcggcgaa gacgggcagc cgctcgcgca cgtccggctc
     4441 tacatccaca gccggtcggg cgccgccgcc gcgcgccagt tgccgcccgc cgagtccgac
     4501 gaagacggca aattcgtctt caacgaactc gaacccggcc tctacggcat caggccctat
     4561 ctgcccggct acaccgccga ggcggagccg agcgagaacc cttcgggcgc caaccaccgg
     4621 ctcggcgacg acgcgacgat ccgcctcgtc cggggcggcg tcatcaccgg caccgtgacg
     4681 cgcgcggacg gcgagccggt ggtcggcgcg aacgtgcgcg tcaaccgcct gcgcacgctc
     4741 gacaaccgcc ccgcgcccgc gctcgacctc tggttcggca acgagtacgc gacggacgac
     4801 cgcggcgtct accgcatcta cgggttgccc gcgggcgtct acgtggtcgc cgcgggcggc
     4861 ggccagcgct ggagctggat gccgcccacg cccttcgacg ccgacgcgcc gacctacttc
     4921 cgctccggca cgctcgacac ggcggaggag gtcaccgtgc gcgccgggca ggaggtcggc
     4981 gccgtagacc tgcgctaccg cggcgagcgc ggctacaccg tcagcggcac ggtcgttctc
     5041 cctccggccc gcaagcccga agactccttc aacctcaaca tcagcctcgt ccacgccgcg
     5101 accggcacgc cgctcgtcaa cacgatgtcg cagtcgcgcg acggcgcgaa cgccttctcg
     5161 ttcgacggca tcgccgacgg cgagtacgag ctgcgcgcct cgcactatac gatggggggc
     5221 gagggcaacg cggcggagcc gctgcgcgtc tccgtcaggg gcgccgacgt gcccggcctc
     5281 aagctcatgc cggcccccct cggcgccatc ggcggcgacc tcgcgctcga accgttgcgc
     5341 gaagcggagg gcgcgtgcaa agcggcggcg cggcgcggtc tcatcccgca ggaggtgctc
     5401 gtcgccgccc tgcgcgagga gcgcgagggc gccgccgagc agcgccggcc cgcgacgccg
     5461 cgacgccgcg agtcggcgcc cgacgagggc ggcgccttcc tcatccgcac gctcgaaccg
     5521 ggccgctacc gcctcgacgc gcggcccgcc gacgaactct ggtacgtgcg cgccatccaa
     5581 ccgcccgccg ccgccccggc gggcacgccc ccggcagcgg ccccttcatc gacgacgagg
     5641 ccgcccgcct cgcgcagaga ctccgccgcg ccgggcgccg cgctcgacct caagccgggg
     5701 cagcagttca agggtctgcg ggtcgtcgtc gccgagggcg ccgcttcgct cagcggtcgc
     5761 gtcgcgcccg ccgcggacga cgcgccgccg ctcccgcccg ccgcgcgcct gcgcgtgcac
     5821 ctcgtcccgg cgcagcgcga gcaggccgac gacgcgctgc gctactacga gacgacgccc
     5881 gcccccgacg gcgccttcgc cttccgccac ctcgcccccg gtcgctacct cgtagtcgtg
     5941 cgcgaggccg aggccgacgc gcccgcgcgc cccgcgggct gggacggcgc ggcgcgcgcg
     6001 cgcctgcgcc gcgaagccga ggccgcgaac ctctccgtcg agcttgagcc ctgccggcgc
     6061 acgaccgact acgcgctgcg cttcaacgcg acgcgctgag agcggcccga cttccactct
     6121 cgtccggggg cgtgcgacgc gcgcctccgg acgggcggct tcatgctcgt ccttcccacg
     6181 cgtccttcac acgccgccgc cgccctctcg aaagattacc ggcgccgctc tcggcagttg
     6241 cgcgagcgcg cgtgaatcag tcatcattgc ccgcaaagtt cttcaagaca ctgaccagcg
     6301 aaaagaaaag gtaacggcat ggactggctg accgacccgc aggcgtggat agggctgctc
     6361 acgctgaccg tcctcgaaat cgttctcggc atagacaacg tcatcttcat ctcgatcctc
     6421 gcgggcaagc tccccgccga gcagcagtcg aaggcgcgca ccatcggcct cgcgctggcg
     6481 atgctgacgc gcatcctgct cctcttctcg atctcgctca tcatgagact cacgacgccg
     6541 ctcttcgagg tgttcgacca cggcgtctcg gggcgcgacc tgatcctgct cgtcgggggg
     6601 ctgttcctca tcggcaaatc gacgcacgag attcactcga agctcgaagg cgaagagggg
     6661 cacgcctcgg cgcgcgtcgc gccgaccttc gcgagcgtca tcatccaaat cctgctgctc
     6721 gacatcgtct tctcgctcga ctcggtcatc accgccgtcg ggatggtctc gaacctgtgg
     6781 gtcatggtca cggcggtcgt catcgccgtc ggcttcatgc tcgtcttcgc cggccccgtc
     6841 agcgccttcg tcgagcgcca cccgaccgtc aagatgctgg ccctgagctt cctcctcctg
     6901 atcggcacga cgctcatcgc cgacggcctc ggcttccaca tcccgaaggg ctacgtctac
     6961 accgcgatgg ccttctccgt cttcgtcgag atactcaacc tcaagatgcg caagcgcaaa
     7021 gccgccaagc ccgtcaagct ccacgaagcc tacacgcaaa cgacggattg acaacgaaag
     7081 gactgaggac tgagtaaaag acaaattgct tcggtgggaa cgaattgctt tcactcgatc
     7141 ctcagtcctc agtcctcagt acttagtctt atgaggcatg aaaacacgct cgacctcggc
     7201 agcacggcgc tcgccgtgat cgacatgcag gagtctttcc ggcaggccat ccccgacttc
     7261 acggagctgg ccgtgcgcgt cgcgcgcgtc gcacacgccg cgcgactgct gggcgtcccc
     7321 gtcctcgtca ccgagcagta cccgaaggga ctcggccaca ccgccgccga gattcgcgcc
     7381 gtgctccccg aggacttcga gccgatggag aagacggcct tcagctcgtg cggcgccgga
     7441 gcgttcgtcg ccgcgctcga acgcgcgggg gcgcgccacg tcctcgtctg cggcatcgag
     7501 gcgcacgtct gcgtcaacca gaccgtgcac gacctgctgg cgggcggcta caaagtccac
     7561 ctcctcacgg actgcatctc ctcgcgcgcc gagcacaacc ggcacgtcgg cctctccaag
     7621 atgcggcagt ccggcgcgct cccctcctcg accgagctgg ccctcttcga actcatgcgc
     7681 gacgcgcgcc acgaacagtt caaggcgatt caaaaactga taaagtagga tatattcatt
     7741 gattaatttg tccattgact ca
//
LOCUS       DBNGOH010000265         3693 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3154934, whole genome shotgun sequence.
ACCESSION   DBNGOH010000265 DBNGOH010000000
VERSION     DBNGOH010000265.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3693)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3693)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3693
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3154934"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(32..1039)
                     /locus_tag="AC159P_05035"
     CDS             complement(32..1039)
                     /locus_tag="AC159P_05035"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HIR3694851.1"
                     /translation="MYKSRGNNLTFVRRNRVAARAALFALLFAAALAPQAFAQPSNIL
                     RYDRHESERSDRVTGGRIERAEFAAGEPDIRLTLNVPSFRLTLWQNDREVKSYFVGVG
                     EKDYPVYIGDREATEVIWNPAWIVPSSDWTRGRKGVRPGQVVKPTNPNNPLGKLKIPL
                     GDGYLIHQAAARTDLGGLVSHGCVRMLRADLYDLAEKIVAARSAPVTRRQIEAAKRGK
                     KTLVARLDEPLPVDINYDTLVVEGGALHIYPDVYDRRLNTPERLRLELQSSGVDVSGL
                     GEATLKGMLAKVTRRTRFVVETSSIEQGRALEDGRVLPLIGNPPKPKPATRKRTTRAG
                     R"
     gene            complement(1364..3256)
                     /locus_tag="AC159P_05040"
     CDS             complement(1364..3256)
                     /locus_tag="AC159P_05040"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HIR3694852.1"
                     /translation="MKNLSPLFAALSLALVIVIPGGAPAVAQRAPQRFAAGAMPEPRF
                     ADPERARKLAAAFPEIERLFNAWVERRRMPGAVMGVVIDGELAWVKGAGVRETEGRAP
                     VNADTVFRIASMTKSFTALAILKLRDEGKLSLDDPASRYVPALAGLEYPTKDSPAVTV
                     RHLLTHSEGFPEDNPWGDRQLAQTNATMDAWMRQGIPFSNAPGVAFEYSNYGFAVLGQ
                     IVARASGKPYEVYVRDEILRPLGMNASYFEATEVPRAHVALGYRREGDQLKPEPLLAH
                     GSFGAMGGLWTSARDLGRYVAFLMSAFPPRDEAERGPVRRASAREMQQAARAENVLAY
                     RAAVDAPLQLSVNSYGYGLGVSQNCRFGYVVGHSGGLPGYGSLMSWLPEHGVGLIAMG
                     NQTYSGFGGLFNDALAALHRTGALQPRVVRPAPALVEAQRDVTQLVLKWDDALASRVA
                     ADNLFMDEPAERRAARLGELATKHGACRPAESALEAENALRGSWKMPCERGWLNVSVT
                     LAPTAPPRVQSLSVRGVIPPGEEMTKAVESVLNLMTGWDAKAAEALLAPGANAEQIRR
                     HVAASSAWGACKVRDHLGGDGARDSTVRLACERGTLIALVSLDPETRRLKGLDLLPTR
                     AERCVP"
     gene            complement(3424..>3693)
                     /locus_tag="AC159P_05045"
     CDS             complement(3424..>3693)
                     /locus_tag="AC159P_05045"
                     /inference="COORDINATES: protein motif:HMM:NF016196.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 family protein"
                     /protein_id="HIR3694853.1"
                     /translation="EEKLRRASEALTERITAAAEERLPAAINEFDIGGIVREKVSGYP
                     VEKLEALVLSVAKQHLKTIELFGAAIGFIIGLMQAGYLYWKLFWH"
BASE COUNT          565 a         1229 c         1286 g          613 t
ORIGIN      
        1 cttcttttct acttctctgt ggcgaaccgt ttcaccgacc cgcgcgcgtc gtgcgcttgc
       61 gcgtcgcggg cttcggcttc ggcgggttgc cgatgagcgg gagcacgcga ccgtcttcga
      121 gtgcgcggcc ctgctcgatg ctgctcgtct cgacgacgaa ccgtgtgcgg cgcgtgactt
      181 tggccagcat ccccttcagc gtcgcttcgc cgaggcccga cacgtccacg cccgacgatt
      241 gcagctccag gcgcaggcgc tcgggcgtgt tgaggcggcg gtcgtacacg tccgggtaga
      301 tgtggagcgc gccgccctcg acgacgagcg tgtcgtagtt gatgtccacg ggcagcggct
      361 cgtcgagccg ggcgacgagc gtcttcttgc cgcgcttggc ggcctcgatc tgtctgcgcg
      421 tgacgggggc cgagcgcgcg gcgacgatct tctcggccaa gtcgtagagg tctgcgcgca
      481 gcatccgcac gcagccgtgc gagacgaggc cgccgaggtc tgtccgcgcc gccgcctggt
      541 ggatgaggta gccgtcgccc aaagggattt tgagcttgcc gagcgggttg ttcgggttgg
      601 tcggcttgac gacctgaccg ggccggacgc ccttgcggcc ccgcgtccag tcgctcgacg
      661 gcacgatcca cgccgggttc cagatgacct cggtcgcctc gcggtcgccg atgtagaccg
      721 ggtagtcctt ctcgccgacg ccgacgaagt aggacttcac ctcgcggtcg ttctgccaca
      781 gcgtgaggcg gaacgagggg acgttcagcg tcaggcggat gtccggctcg cccgccgcga
      841 actcggcgcg ctcgatgcgc ccgcccgtca ccctgtccga gcgctccgac tcgtggcggt
      901 cgtagcggag aatgttcgac ggctgcgcaa aggcttgcgg cgcgagcgcg gcggcgaaca
      961 gcagcgcgaa gagcgccgcc cgcgcggcca cgcgatttcg acgcacgaat gtcaggttgt
     1021 tgccccttga tttgtacata gaatgttttc ttgcccgcgc cgcccgcagg gagaggagac
     1081 gcagccgggc cttggttcgc aacccccttc gtcgccgggg gtttaacgtc cgtcctgctc
     1141 gggggacgat acgcgcatag cttaccacag acggggaaac agtaggcagt aggcggtagg
     1201 cagttaaagc aagaagggcg aagaagcttg agaggtaaag tcccgagcct ctgcgccctt
     1261 cgagcaataa aggaagaggc ttgagacgtt gcatctcaag cctcttcctt attctgtctt
     1321 aactgcctac cgcctaccgc ctaccgccta ccgcctactg ttcttaaggg acgcagcgct
     1381 cggcgcgggt cggcaggagg tcgagtcctt tgaggcggcg cgtctcgggg tcgagcgaga
     1441 cgagggcgat gagcgtgccg cgttcgcagg cgagacgcac ggtgctgtcg cgcgcgccgt
     1501 cgccgccgag gtggtcgcgc accttgcagg cgccccacgc ggaggaggcc gcgacgtgcc
     1561 gccgtatctg ctccgcgttg gcgccgggcg cgaggagcgc ctccgccgcc ttcgcgtccc
     1621 aaccggtcat cagattgagg acggactcga ccgccttcgt catctcctcg cccggcggga
     1681 tgacgccgcg cacggagagc gactgcacgc gcggcggcgc ggtcggcgcg agcgtgaccg
     1741 agacgttcag ccagccgcgc tcgcagggca tcttccagga accgcgcagc gcgttctccg
     1801 cttcaagcgc cgactcggcg ggcctgcacg cgccgtgctt cgtcgccagc tcgccgagcc
     1861 gcgcggcgcg tcgctctgcc ggctcgtcca tgaagaggtt gtcggcggcg acgcgcgagg
     1921 cgagcgcgtc atcccacttc aagacgagct gcgtgacatc cctctgcgct tcgacgagcg
     1981 cgggtgcggg gcggacgacg cgaggctgga gcgcgcccgt gcggtggagc gcggcgagcg
     2041 cgtcgttgaa cagcccgccg aagccggagt aggtctggtt gcccatcgcg atgaggccga
     2101 cgccgtgctc gggcagccag ctcatcagag agccgtagcc gggcagcccg ccgctgtggc
     2161 cgacgacgta gccgaagcgg cagttctgcg agacgccgag gccgtagccg tacgagttca
     2221 cgctgagttg gagcggcgcg tccaccgccg cacggtaagc cagcacgttc tcggcgcgcg
     2281 ccgcctgctg catctcgcgc gcgctcgcgc gccggacggg gccgcgctcg gcctcgtcgc
     2341 gcggcgggaa ggccgacatc aggaacgcga catagcgccc gaggtcgcgc gccgaagtcc
     2401 acaaaccgcc catcgcgccg aacgagccgt gcgcgagcag aggctccggc ttcaactggt
     2461 cgccctcgcg gcggtagccg agcgcgacgt gtgcgcgcgg gacttcggtc gcctcgaagt
     2521 acgaagcgtt catgccgagc gggcgcagga tttcgtcgcg cacgtaaacc tcgtaaggct
     2581 tacccgaagc gcgcgcgacg atctggccga ggacggcgaa accgtagttc gagtactcga
     2641 aggcgacgcc cggcgcgttc gagaacggga tgccctgacg catccacgcg tccatcgtcg
     2701 cgttcgtctg cgcgagttgg cggtcgcccc acgggttgtc ttcggggaag ccctcggagt
     2761 gcgtcagtag atgccggacg gtgacggcgg gagagtcttt ggtcgggtac tcaagccccg
     2821 cgagcgcggg gacgtagcgc gacgccgggt cgtccagaga gagcttgccc tcgtcgcgca
     2881 gcttcaggat cgccagcgcc gtgaagctct tcgtcatcga ggcgatgcgg aacactgtgt
     2941 cggcgttgac gggcgcgcgc ccctcggtct cgcgcacgcc cgcgcccttg acccacgcca
     3001 gctcgccgtc gatgacgacg cccatcaccg cgcccggcat ccgccgccgc tcgacccacg
     3061 cgttgaagag tctttcgatc tcggggaagg ccgccgccag cttgcgcgcg cgctccgggt
     3121 ccgcgaaccg cggctccggc atcgcgcccg ccgcgaaacg ctgcggcgcg cgctgtgcga
     3181 cggcgggcgc gccgccgggg atcacgatga cgagcgccag cgacagcgcg gcgaagagcg
     3241 gcgaaagatt tttcatgaca actccgaagc gaaaaaataa gtgatgagga ctgaggactg
     3301 aggactgagc gataagagga agcggtgacg agtgatgaga gtcctgctgt tcggactcgt
     3361 cacggtttac ggtttacggc tcacgtcttt ttctcagtcc tcagtcctca gtcctttgtg
     3421 ttatcagtgc cagaaaagtt tccagtagag gtagccggcc tgcatcagcc cgatgatgaa
     3481 gccgatggcg gcgccgaaca gctcgatggt tttgaggtgc tgcttggcga ccgagagcac
     3541 gagcgcttcc agcttctcga cggggtagcc cgacaccttc tcgcgcacga tgccgccgat
     3601 gtcgaactcg ttgatggccg ccggcagccg ctcctcggcc gccgccgtga tgcgttcggt
     3661 cagagcctcg ctcgcgcggc gcagtttctc ttc
//
LOCUS       DBNGOH010000266         9113 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_406130, whole genome shotgun sequence.
ACCESSION   DBNGOH010000266 DBNGOH010000000
VERSION     DBNGOH010000266.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 9113)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9113)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9113
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_406130"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3650)
                     /locus_tag="AC159P_05050"
     CDS             complement(<1..3650)
                     /locus_tag="AC159P_05050"
                     /inference="COORDINATES: protein motif:HMM:NF013508.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdrD B-like domain-containing protein"
                     /protein_id="HIR3694854.1"
                     /translation="MLNARLRPVARALALTLLVAFTAAGRVGAVARDGGTEAGTVISN
                     TAEATYEDATGAGFRTVSPTVTVTVRSVSALTVTPDETEPSATVSPNERVTRLFRVCN
                     TGNVPDLYTITRAEVSAPAALVSLHFDRDAGGTLDEADALIQLNQTLSPRVARAACVG
                     VLAVVDTNASRPGSHLTINLTARSNATDVLSGATEDAGQIINAVGNGARFSSPDDPAL
                     PPVKLVEGRGTLTAAHGQALSYTISFRNSGDIAARNVRLADDLPAGLDYVPGSLRLGT
                     RALTDAEDADEGFYKNRHLEVRLAEVAVGELVVVSFRARVLGTVPPGQGLVNTALVVA
                     DNTPAAASSTATAVVNPFGLVYQGRSAGTPIPGARVALLADSTTGALVSLGGDGSEPN
                     GGNANPFSSDGQGRFSFVLSPAQLGTAQLPARYFLNVTAVGYRARMLELSVTPEGSTG
                     LFTLTVRALDGQPVAGAGGFELVETAVSIERLAAFALNVPMFENTTLEITKTVDRPSA
                     EIGDVVSYRVELHNATAAAVNDAVVRDVLPTSFHYAAGTARLEVPSAAPRSIEPNVEP
                     GGVLVFNLGTIPSGGRAFVSYRVRIGANAREGDQFNTATGTGTLASGERLSTPTARTP
                     VRVRRGVFSSQQIILGRVFEDANLNGQFDKGERGVSGVRIYLNNGQSVITDSEGLYNF
                     PSVNEGAQVLSLDPVTLTDGYRLAETDRRDEQSWTRLLRTPLGGGALLRQNFALRAPD
                     REARDNASANATANKSAFTSDADKRDSRAPQTAMRGSLFGGLTPDGREAAPSSSTNAG
                     GTNGASNHGTNGTNGHGTNGHGETPKSEEASALAAGTHELATNETLEPLAPGAVKILS
                     PQAEETIAGAALEVEARAHGAWTVAVKVEGQRVPDSKLGERRVDHKNNLATFRFVGLN
                     VRPGPNRIKVTAVGPAGETGQSAEVTAYGRGPAKRIEIVTDKAQIAAGGRDRARVRVR
                     AFDQWGRPAADGAVALEVTAGRLVRVEEKDAAADKGEKKDRQSPVADDELTNGGPLRQ
                     RMLTLTGGEGLVDLIADGEPGEAEIRATTGVLEAHKRVRVIPEVRPAILVGLAEVTIG
                     SGAPELTNDDGRDMRSRLAFFYRGQVFGSNLFTLAYDSNRALNRTAGRDRLFQFDPHD
                     RAYPIFGDSSTRFEDAQSNSKLYARLDRGRSYFLFGDFDTDSKDAGLASYNRRLTGAK
                     LHLENAAGDFISV"
     gene            complement(3739..5853)
                     /locus_tag="AC159P_05055"
     CDS             complement(3739..5853)
                     /locus_tag="AC159P_05055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="beta strand repeat-containing protein"
                     /protein_id="HIR3694855.1"
                     /translation="MNNHKITTLIRTAARALLVAAFVCAMTATAALAQTTSGGTVISN
                     TASASYSDGTNSYTTVSNTVTVTVANVSGLAITPDAGSVPTVVAGQTGVDFTFTVTNT
                     SNFSNQVRFLASGASVQATGPVTVTAAFIDTDLNGIDAGDTDIFANGADVLSATIARN
                     ATLTVIVRVSVNAAATAGAAINVRLGDSAAGGPTFDNQTANSSANEVRTSVPSGTTAP
                     VNGESEARGDISTTVENDAQLRLNVNYPAGPVALGSNITYTWSLQNTGARPVSAQTLA
                     GAPGGSNTGVFVVAPVPVNTTFVSVTPPAGVTVLYTTSSLANDPVGASTVWTTTPPAS
                     GVTRVAFNTGASLAAGASVVNMQFVVQVNTGINASNPIWEIGDAFGLNSVSANITDQS
                     EDAVSNKGDGNANFNEPRLGIDAPNATQGIQVPTTLVAVGSVLLGPVGFPGAVGPTNN
                     NDDYTNRTTSAASFAGVAPGGSTVAASTVDFVNTVQNTGNANDTFTFTAPTVPAGFTV
                     SISTDGGTNFTVVSGGGSTTLAVGFGGSANIIVRVAAPSGQTVLTGFETVIRATSANT
                     PASANDTIDRLYTGFLRLVKSVVVDNTTGVGGATDAVPGADIVYTITYTNVSSSGGTN
                     NVTLTASNIVINEDGDAAPNNWAASTTQVTSPAPSDSTTGTITDGDTNGAVTATTTFL
                     RDSIPSLAPGAGGTFVFRRRIN"
     gene            complement(5981..6511)
                     /locus_tag="AC159P_05060"
     CDS             complement(5981..6511)
                     /locus_tag="AC159P_05060"
                     /inference="COORDINATES: protein motif:HMM:TIGR01451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694856.1"
                     /translation="MIGLKKKLGALMPLALCLLVLCGVGALAQRQLLPSAGAGARPDI
                     KVALSGAVEREGQQIALDKAQAVQPGEILSWHITSANDGDGAARDYKAVGHIPAGTTF
                     VAGSAAGENGSTVTYSIDGGKTFSTQPIVEERQADGTVKRVPAPVSMYTEVRYEWSDA
                     LAAGGKLSASYKVRVK"
     gene            complement(6620..6997)
                     /locus_tag="AC159P_05065"
     CDS             complement(6620..6997)
                     /locus_tag="AC159P_05065"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3694857.1"
                     /translation="MARVMICDDDCAMAEGLVAALRAAGHEAETCRHTMDVLRAAADG
                     RFDVIVIGLDMPGFGRAGAVEALREIAPETAIIAIHNRPSEIMRASRHARLAAVLPRP
                     VRLKDFMYAVACALKEHAHLEPA"
     gene            complement(7337..8755)
                     /locus_tag="AC159P_05070"
     CDS             complement(7337..8755)
                     /locus_tag="AC159P_05070"
                     /inference="COORDINATES: protein motif:HMM:NF025885.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HIR3694858.1"
                     /translation="MGRKIRVMVVDDDAVLRRLVSDQVGRMGFDATAAASGEEALDAL
                     AKSDFDVVLLDIRMPGLSGLDALRAIRRLEEPPEVIMLTADTALPTGIEAMRLGAYDY
                     LTKPATLDEIEAVIGKADEKRRLVRQNVALRDAVVRAPAQEDAPAPVYRSAAMEGLVA
                     LAETAARADSTVIITGESGTGKDVLARFIHARSSRKAAPMIAVNCAALPETLFESEFF
                     GHERGAFTGATATKRGLIEAADSSSLFLDEVGEMPQQTQVKLLRFLEEGHFRRVGSTR
                     DRAADVRVIAATNRNLAEDVSAGRFRADLFYRLNVISLHVTPLRRRREDIPVLVEHFL
                     STFRERFNRPALELSADARAALEAYDWPGNVRELRNALERASALASADTLEAEHVLGD
                     KARASAADPSSAHTTDGPQPSAHTPTLEELERRHILRVLEESSGNRERAAAILGISAR
                     TLYRKLREYETSAAPGDLATEE"
BASE COUNT         1247 a         2835 c         3513 g         1518 t
ORIGIN      
        1 acggagatga agtcgccggc ggcgttttcg aggtgaagct tcgcgcccgt cagcctgcgg
       61 ttgtaagaag cgaggccggc gtccttgctg tcggtgtcga agtcgccgaa gaggaagtac
      121 gagcgaccgc ggtcgaggcg cgcgtagagc ttggagttcg attgcgcgtc ttcgaagcgc
      181 gtcgaggagt cgccgaagat ggggtaggcg cggtcgtgcg ggtcgaactg gaagaggcgg
      241 tcgcgcccgg cggtgcggtt gagcgcgcgg ttgctgtcgt aggcgagcgt gaagaggttc
      301 gagccgaaga cctgcccgcg gtagaagaag gcgaggcggc tgcgcatatc cctgccgtcg
      361 tcgttggtga gttcaggagc gccgctccct atcgtcacct cggcgaggcc gacgaggatc
      421 gcgggccgca cttcggggat gacgcgcacg cgcttgtgcg cttcgagcac gccggtggtc
      481 gcgcgtatct cggcctcgcc cggctcgccg tcggcgatca aatcgacgag accttcgccg
      541 cccgtcagcg tcaacatccg ctgccgcagc ggcccgccgt tcgtgagttc gtcgtcggcg
      601 accggactct gcctatcttt cttctcgccc ttgtccgccg cagcgtcctt ctcttcgacg
      661 cgcacgaggc ggcccgcggt gacttcgagc gcgacggcgc cgtccgccgc gggccgtccc
      721 cactggtcga aggcgcgcac gcgcacccgc gcgcggtcgc gcccgcccgc cgcgatctgc
      781 gctttgtcgg tcacgatctc gatgcgtttg gcggggccgc gaccgtaggc cgtgacctcg
      841 gcggactggc cggtctcgcc cgcggggccg acggcggtga ctttgattct gttcgggccg
      901 ggccgcacgt tgaggccgac gaagcggaag gtggcgaggt tgttcttgtg atcgaccctg
      961 cgctcgccga gcttggagtc ggggacgcgc tggccttcga ccttgacggc gaccgtccac
     1021 gcgccgtgcg cgcgcgcctc gacttcgagc gccgcgcccg cgatggtctc ctcggcctgc
     1081 ggcgacagaa tcttcaccgc gcccggcgcc agcggctcaa gcgtctcgtt cgtcgccagc
     1141 tcgtgcgtgc ccgccgcgag cgccgaagcc tcttcgctct tcggcgtctc gccgtggccg
     1201 ttcgtcccgt ggccgttcgt cccgttcgtc ccgtgattgc tcgcgccgtt cgttccgccc
     1261 gcgttggtgc tcgatgaagg cgccgcttcc ctgccgtcag gcgtgagacc gccgaacagc
     1321 gagcctctca tcgccgtctg cggggcgcga ctgtcgcgct tgtcggcgtc ggaagtgaag
     1381 gcggacttgt tcgccgtcgc gttcgcgctc gcgttgtcgc gcgcttcccg gtcgggcgcg
     1441 cgcagcgcga agttctggcg gaggagcgcg ccgccgccga gcggcgtgcg cagcagacgc
     1501 gtccagctct gctcgtcgcg ccggtcggtc tcggcgaggc ggtagccgtc ggtgagcgtc
     1561 acggggtcga gcgagagcac ctgcgcgcct tcgttgacgg aggggaagtt gtagagacct
     1621 tcggagtcgg tgatgaccga ctggccgttg ttgaggtaga tgcggacgcc cgagacgccg
     1681 cgctcgccct tgtcgaactg gccgttgagg ttcgcgtcct cgaagacgcg gcccaagatg
     1741 atctgctgcg aagagaagac gccgcggcgg acgcggacgg gcgtgcgcgc ggtcggcgtc
     1801 gagaggcgct cgcccgacgc gagcgtcccc gttccggtcg cggtgttgaa ctggtcgccc
     1861 tcgcgcgcgt tggcgccgat gcggacgcgg tagctcacga aggcgcggcc cccggaagga
     1921 atcgtcccga ggttgaagac gagcacgccg cccggctcga cgttcggctc gatggagcgc
     1981 ggcgcggcgg acggaacttc cagacgcgcg gtgcccgcgg cgtagtggaa agaggtcggg
     2041 agcacgtcgc gcacgaccgc gtcgttcacg gcggcggcgg tcgcgttgtg cagctcgacg
     2101 cgataggaga ccacgtcgcc gatctctgcc gaaggccggt caacggtctt cgtgatttcg
     2161 agcgtggtgt tctcgaacat cggcacgttc agggcgaagg cggcgaggcg ttcgatggag
     2221 acggcggtct cgaccagctc gaagccgccg gcccctgcga cgggctggcc gtcgagggcg
     2281 cgcaccgtca acgtgaagag gccggtcgag ccttcgggcg tgacggagag ttcgagcatg
     2341 cgcgcgcggt agcctacggc ggtgacgttg aggaagtagc gcgcgggcag ttgcgcggtg
     2401 ccgagctgcg cgggcgagag gacgaagctg aaacgccctt gtccgtccga cgagaagggg
     2461 ttcgcgttgc cgccgttcgg ttcggagccg tcgccgccca gagagacgag cgcgccggtc
     2521 gtcgagtcgg cgagcagcgc gacgcgcgcg cccggtatcg gcgtgcccgc gctgcggccc
     2581 tgatagacga ggccgaaggg gttgacgacc gccgtcgcgg tgctgctcgc ggcggcgggc
     2641 gtgttgtccg cgacgacgag cgccgtgttg acgagtccct gaccgggcgg caccgtgccg
     2701 agcacgcgcg cccggaacga gacgacgacc agctcgccga cggcgacctc ggcgagccgg
     2761 acttcgaggt ggcggttttt gtagaagcct tcgtcggcgt cctcggcgtc ggtcagcgcg
     2821 cgcgtgccga ggcgcagcga gccgggcacg tagtcgagac cggcgggcag gtcgtcggcg
     2881 aggcggacgt tgcgcgccgc gatgtcgccg ctgttgcgga aagagatggt gtacgagagc
     2941 gcctgcccat gcgcggcggt cagtgtgccg cggccttcga cgagcttgac gggcggcagc
     3001 gcggggtcgt cgggcgaaga gaatcgcgcg ccgttgccga cggcgttgat gatctgcccc
     3061 gcgtcttcgg tcgcgccgct cagcacgtcg gtcgcgttcg agcgcgcggt gaggttgatc
     3121 gtcaggtgcg agccgggccg gctcgcgttc gtgtccacga cggcgagcac gccgacgcac
     3181 gcggcgcgcg cgacgcgcgg actcaacgtt tgattaagtt ggatgagtgc gtccgcctcg
     3241 tcgagagtgc cgccggcatc ccggtcgaag tggagcgaga cgagcgcggc gggcgcgctc
     3301 acttcggcgc gcgtgatggt gtagaggtct gggacgttgc cggtgttgca gacgcggaag
     3361 aggcgcgtga cgcgctcgtt cggggagacg gtggccgaag gctccgtctc gtcgggcgtg
     3421 accgtcaggg ccgagaccga gcgcacagtg accgtgacgg tcggcgagac ggtgcgaaag
     3481 cccgcgccgg tcgcgtcctc gtaggtcgcc tcggcggtgt tggaaataac ggtgcccgcc
     3541 tccgtccccc cgtcccgcgc gacggcgccc acgcgtcccg ccgccgtgaa ggcgacgagg
     3601 agcgtgagcg ctaacgcgcg ggcgacgggg cggaggcggg cgttaagcat tgtggagaag
     3661 accgattgtc agaggtcgga ggtcagtgag aatcagtttt taactgacct ccgacctccg
     3721 acctccgacc tctgccgttt agttgatcct gcggcggaac acgaaggtgc cgccggcgcc
     3781 cggagcgagg ctcgggatgc tgtcgcgcag gaaggtggtg gtggcggtga cggcgccgtt
     3841 ggtgtcgccg tcggtgatgg tgccggtcgt cgagtcggac ggggccgggc tggtgacctg
     3901 ggtcgtgctg gcggcccagt tgttgggggc ggcgtcgccg tcttcgttga tgacgatgtt
     3961 cgacgccgtc agcgtgacgt tgttggtgcc gcccgaggac gagacgttgg tgtaggtgat
     4021 ggtgtagacg atgtccgcac cgggcacggc gtcggtcgcg ccgccgacgc ccgtcgtgtt
     4081 gtcaacgacc accgatttga cgagccgcag gaagccggtg tagagccggt caatcgtgtc
     4141 gttcgccgag gcgggggtgt tggccgaggt ggcgcggatg acggtctcga agccggtcag
     4201 cacggtctgg cccgagggcg ccgcgacgcg cacgatgatg ttcgccgagc cgccgaaacc
     4261 gacggcgagc gtggtgctgc cgccgcccga gacgacggtg aagttggtgc cgccgtcggt
     4321 gctgatcgag acggtgaagc ctgcgggcac ggtcggcgcg gtgaacgtga acgtgtcgtt
     4381 ggcgttgccg gtgttctgca ccgtgttgac gaaatcgacg gtggaagccg cgacggtcga
     4441 gccgcccgga gcgacgcccg cgaacgaggc cgccgaggtc gtccggttcg tgtagtcgtc
     4501 gttgttgttg gtcgggccga ccgcgccggg gaagccgacc gggccgagca ggacggagcc
     4561 gaccgccacg agcgtggtcg gaacctgtat accctgcgtc gcgttcggcg cgtcgatgcc
     4621 gaggcgcggc tcgttgaagt tggcgttgcc gtcgcccttg ttcgagacgg cgtcctcgga
     4681 ctggtcggtg atgttggcgc tcaccgagtt gagaccgaag gcgtcgccga tctcccagat
     4741 cgggttcgag gcgttgatgc cggtgttgac ctgcacgacg aactgcatgt tcaccacgga
     4801 cgcgccggcg gcgagcgagg cgcccgtgtt gaaggcgacg cgcgtcacgc ccgaggcggg
     4861 cggcgtggtc gtccacacgg tcgaggcgcc gacggggtcg ttggcgagcg aggaggtcgt
     4921 gtagaggacg gtcacgcccg cgggcggtgt gaccgagacg aaggtcgtgt tgacgggcac
     4981 gggggcgacg acgaagacgc cggtgttcga gccgccgggc gcgcccgcga gcgtctgcgc
     5041 cgagacgggg cgcgcgccgg tgttctggag cgaccacgtg taggtgatgt tcgagccgag
     5101 ggcgacgggg ccggcggggt agttgacgtt caggcggagc tgcgcgtcgt tctcgaccgt
     5161 cgtcgagatg tcgccgcgcg cctccgactc gccgttgacg ggggccgtcg tgcccgaagg
     5221 cacggaggtg cggacttcgt tggcggagct gttggccgtc tggttgtcga aggtcgggcc
     5281 gcccgccgcg gagtcgccga ggcggacgtt gatggccgcg ccggcggtgg ccgcggcgtt
     5341 gacgctgacg cggacgatga ccgtcagcgt cgcgttgcgc gcgatggtcg ccgagagcac
     5401 gtccgcgccg ttggcgaaga tgtcggtgtc gcccgcgtcg atgccgttga ggtccgtgtc
     5461 gatgaacgcc gccgtcacgg tgacggggcc ggtcgcctga acggacgcgc ccgaggcgag
     5521 gaagcgcacc tgattcgaga agttgctcgt gttggtgacg gtgaaggtga agtccacgcc
     5581 ggtctggccg gcgacgaccg tcgggacgct cccggcgtcg ggcgtgatgg cgaggcccga
     5641 gacgttggcc accgtgacgg tgacggtgtt cgagacggtg gtgtagctgt tggtgccgtc
     5701 ggagtatgag gccgaggccg tattgctgat gacggtgccg ccgctggtgg tctgcgccag
     5761 cgcggcggtc gcggtcatcg cacacacgaa ggccgcgacc agcagggcgc gggcggcggt
     5821 gcggatgagg gtggttatct tatggttgtt catcttgatt gtcgtcctcg atggtggtcg
     5881 gtgggtctgg cggcgtcacc ttcggcgggc gcgggttcgg gcgcggggaa acttcctttc
     5941 gctccgagat gcccggcacg gggacgcgcg gggtgccctg ttatttgacg cgcactttat
     6001 acgaggcgga gagcttgccg ccggcggcca gggcgtccga ccactcgtag cgcacttccg
     6061 tgtacatgga gacgggcgcg ggcacacgct tcacggtgcc gtccgcctgc cgctcttcga
     6121 cgatgggctg cgtggagaag gtcttgccgc cgtcgatgga gtaggtgacg gtcgagccgt
     6181 tctcgcccgc ggcgctgccc gcgacgaagg tggtgcccgc cgggatgtgg ccgacggcct
     6241 tgtagtcgcg cgcggcgccg tcgccgtcgt tcgcggaggt gatgtgccag ctcaggattt
     6301 cgccgggctg gacggcctgc gccttgtcga gcgcgatctg ctggccctcg cgctcgaccg
     6361 cgcccgagag ggcgactttg atgtcggggc gcgcgcccgc gccggctgaa ggcagcagtt
     6421 gcctctgcgc cagcgcgccg acgccgcaca gcaccagcag gcacagggcc agcggcatga
     6481 gcgcaccgag tttcttcttc aatccaatca tgggtcttct ccttaactag tgtgtccgaa
     6541 agtgcgcgag gcagcgcgcg tgggttggtg cggggccggt cagccggtgg gcgctcgcgg
     6601 ccctcggtga gtttttttat caggcgggtt cgagatgcgc gtgctctttg agggcgcacg
     6661 cgaccgcgta catgaagtcc ttgaggcgga cggggcgcgg cagcaccgcc gcgaggcgcg
     6721 cgtggcgcga ggcgcgcatg atctcggacg ggcggttgtg gatggcgatg atggcggtct
     6781 cgggcgcgat ctcgcgcagc gcctcgacgg cgcccgcgcg cccgaagccc ggcatgtcca
     6841 gcccgatcac gatcacgtcg aagcgcccgt ccgccgccgc cctgagcacg tccatcgtgt
     6901 gacggcacgt ctcggcctcg tgcccggcgg cccgcagagc ggccaccagc ccttcggcca
     6961 tcgcacaatc gtcgtcgcaa atcatcacac gtgccatcgg tttatccgtc ttccttttcc
     7021 ctgctcattc cctacggccc gcgcctgagc acacgggcgc cgccctcccg attaacaagc
     7081 cgcgtaccgc gccgcacgaa gcgccgccgg acaagctaag tcgaagactg aatggattta
     7141 ggcggatgtg tggcggtagc gcgtgggcgc tcggcacgag gcgattgaca aacgcggctg
     7201 acgttatggc aggcggcctg acaaaatgac agtaggcagt aggcggtagg cagttaaagc
     7261 aaggaacacg gaaaggcttg ggacttatca cccaagcctc tcggtctttc tatctttgct
     7321 gcctaccgcc tactgcctac tcttcggtgg cgaggtcgcc gggggcggcg ctggtttcgt
     7381 attcgcggag cttgcggtag agggtgcggg cggagatgcc gaggatggcg gcggcgcgtt
     7441 cgcggttgcc gctcgattct tcgaggacgc ggaggatgtg gcggcgttcg agttcttcga
     7501 gggtcggtgt gtgggccgag ggttgcgggc cgtcggtcgt gtgggcggag gaagggtcgg
     7561 cggcggaggc gcgggccttg tcgccgagga cgtgttcggc ttcgagtgtg tcggcggagg
     7621 cgagcgcgga ggcgcgctcc agggcgttgc gcagctcgcg gacgttgccg ggccagtcgt
     7681 aggcttcgag ggcggcgcgg gcgtccgcgg acagttcgag cgcggggcgg ttgaagcgct
     7741 cgcggaaggt ggagaggaag tgttcgacga ggacggggat gtcttcgcgg cgccggcgca
     7801 ggggcgtgac atggagcgag atgacgttga ggcggtagaa gaggtctgcg cggaagcgtc
     7861 cggcgctcac gtcttcggcg aggttgcggt tggtggcggc gatgacgcgc acgtccgccg
     7921 cgcggtcgcg cgtcgagccg acgcggcgga agtgtccctc ttcgagaaag cgcaggagct
     7981 tgacctgcgt ctgctgcggc atctcgccga cctcgtcgag gaagagcgag gaggagtcgg
     8041 cggcttcgat gaggccgcgc ttggtggcgg tcgcgccggt gaaggcgccg cgctcgtggc
     8101 cgaagaactc ggactcgaag agcgtctcgg ggagcgcggc gcagttgacc gcgatcatcg
     8161 gcgcggcctt gcgcgaagag cgcgcgtgga tgaagcgcgc gagcacgtcc ttgcccgtgc
     8221 ccgactcgcc cgtgatgatg acggtcgagt cggcgcgcgc cgccgtctcc gcgagcgcga
     8281 ccagtccctc catcgccgcg ctccggtaga cgggcgcggg cgcgtcctcc tgcgcgggcg
     8341 cgcgcactac cgcgtcgcgc agcgcgacgt tctgccggac gaggcggcgc ttctcgtcgg
     8401 ccttgccaat gacggcctcg atctcgtcga gcgtcgcggg cttggtcagg taatcgtaag
     8461 cgccgagccg catcgcctcg atgccggtcg ggagcgccgt gtccgcggtg agcatgatga
     8521 cctcgggcgg ctcttcgaga cggcggatcg cgcgcagcgc gtcgaggccc gacaggcccg
     8581 gcatgcggat gtcgagcagc accacgtcga agtcgctctt cgcgagcgcg tccaaagcct
     8641 cctcgccgga ggccgccgcg gtcgcgtcga atcccattcg cccgacctga tccgacacga
     8701 gccggcgcag caccgcgtcg tcgtcaacca ccatcacgcg aatcttgcgg cccatagaat
     8761 cagcgacgag cgggcagaga cgtgaagaga taggcagagc gtcgggtcgc gacatcttat
     8821 cacttgtcgc gcgccgaccg tcacactact ttgagcggcg ccggttcgac acaaccgtta
     8881 cccgccccgc gcctcgtgct cgcgccgtgc ggtgcggtat aacacgtcgg cgggcggcgc
     8941 gccgcccgga gcttggaggc tcgaaaatga atctcgactt ctacatgacg cgtctcgccg
     9001 cgaacgcggc ggtcttcgga ggactcgtcg cgggcgtcgg taaggagcag gcgcgttgga
     9061 agccgacgcc ggagcgctgg tcgctggcgg aggtgatggg ccacgtgcac gac
//
LOCUS       DBNGOH010000267         2852 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_406215, whole genome shotgun sequence.
ACCESSION   DBNGOH010000267 DBNGOH010000000
VERSION     DBNGOH010000267.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2852)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2852)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2852
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_406215"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..247
                     /locus_tag="AC159P_05075"
     CDS             <1..247
                     /locus_tag="AC159P_05075"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004061 - arylformamidase activity
                     [Evidence IEA];
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006569 - L-tryptophan catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HIR3694859.1"
                     /translation="ARALVRRGVRLVGFDYLSVEKFKSDDFETHMVLLTEGVVIVEGL
                     DLRGVTAGSYELVCLPLKIAAGSGDGAPARAILRASD"
     gene            325..684
                     /locus_tag="AC159P_05080"
     CDS             325..684
                     /locus_tag="AC159P_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007933177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR3694860.1"
                     /translation="MEKVNLAEKFSLFEEHWSPKIAGEVNDSYVKLVKFTGEFVWHHH
                     EAEDEMFLVVKGSMTMRLRDRDIEVGEGEFVIIPRGVEHMPVAAAEAHVVLFEPKTVL
                     NTGNVTNERTRPELERV"
     gene            711..1109
                     /locus_tag="AC159P_05085"
     CDS             711..1109
                     /locus_tag="AC159P_05085"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012694429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HIR3694861.1"
                     /translation="MSEPNESRLDVDSPRYRARVYEIVRRIPAGRVMTYGQIAELLGE
                     GYTARTVGFVMHAAEEGVPWHRVINAQGACSTGRVVLPPDKQQRMLEAEGVAFDARGR
                     CDLGRYRWTPPEFEGEEAAGEEPQPSLFTD"
     gene            1140..1550
                     /locus_tag="AC159P_05090"
     CDS             1140..1550
                     /locus_tag="AC159P_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019336307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjQ family protein"
                     /protein_id="HIR3694862.1"
                     /translation="MSNELQKRAPYNISEPQYSVSPQMVTTAFELPGYRVRQNLGVVR
                     GIVVRSRNLFVNIGAFFNTVVGGNITAWSNLCETTRKDAFDIMIQHATELGANAIIGA
                     RYDATEIASGASEVLAYGTAVVVEPLEDTGIYNS"
     gene            1547..1969
                     /locus_tag="AC159P_05095"
     CDS             1547..1969
                     /locus_tag="AC159P_05095"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF025892.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0047617 - fatty acyl-CoA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HIR3694863.1"
                     /translation="MSGDALTDEELARIHEAFAQVPYTRLLGLEFVSASRGAAVYRLT
                     VREELTRMRGILHGGAVASLMDTAAAFAVHTLLEPAQWTVTVDLTLHFLRAASSGQLT
                     AHARVVRNGRRLVILTIEAKDESDQLIAIASTTYSKQV"
     gene            2169..>2852
                     /locus_tag="AC159P_05100"
     CDS             2169..>2852
                     /locus_tag="AC159P_05100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694864.1"
                     /translation="MLSLYSALLAQHPRQLTPVRCAEQTIAQLHRYFEDVVLENNLSA
                     LVVEGLLPEAQRSLRDVARVRQVGRSARYAFFFVSPDDGLTKLDLHEQRHSGGAPCLM
                     KKVTSTPFDERFVVIADARFSALLASVRAHTEEGQDAGGGVEVIWTFEPDIVYSALEY
                     LMARVTAEHPFQAAAFSKAVSTSMPKTTSLQLTVSVTTKLARLLQEQAGREIAVNRIA
                     TAIRSSLELP"
BASE COUNT          504 a         1023 c          872 g          453 t
ORIGIN      
        1 ggcgcgcgcc ctcgtccgtc gcggcgtgcg gctcgtcggc ttcgactacc tgtcggtcga
       61 gaagttcaag tccgacgact tcgagacgca catggttctg ttgacggaag gcgtcgtgat
      121 cgtcgaaggt ctcgacctgc gcggggtcac agccgggagc tacgagctgg tctgcctccc
      181 gctcaagatc gccgcgggca gcggcgacgg cgcgccggcc cgcgccatcc tgcgcgcgtc
      241 ggactgaacg gcgccgcggt tccgctcccg ttcgaaatca tcatccgccg attcggtgaa
      301 aaacatatgt ctcgacaacc cccgattgag aaggtcaacc tcgccgagaa gttcagcctc
      361 ttcgaagagc attggagtcc gaagatcgcg ggcgaggtca acgactctta cgtgaagctc
      421 gtcaagttca cgggcgagtt cgtctggcac caccacgagg ccgaggacga gatgttcctc
      481 gtcgtcaaag gctcgatgac gatgcgcctg cgcgaccgcg acatcgaagt cggcgagggc
      541 gagttcgtca tcatcccgcg cggcgtcgag cacatgcccg tcgccgccgc ggaggcgcac
      601 gtcgtcctct tcgagccgaa gaccgtcctc aacaccggca acgtcaccaa cgagcggacg
      661 cgccccgaac tcgaacgcgt ctaacgccgg acgagcacac ccaacacatc atgagtgaac
      721 cgaacgagag ccgcttggac gttgactcgc cgcgctaccg cgcgcgcgtc tacgagatcg
      781 tgcgccgcat ccccgcgggc cgcgtgatga cctacgggca gatcgcggag ctgctcggcg
      841 agggctacac ggcgcgcacc gtcggcttcg tcatgcacgc ggctgaagag ggcgtgccgt
      901 ggcaccgcgt catcaacgcg cagggcgcgt gctcgacggg tcgcgtggtg ctgccgcccg
      961 acaagcagca gcgcatgctc gaagccgagg gcgtcgcgtt cgacgcgcgc gggcgctgcg
     1021 acctcgggcg ctaccgctgg acgccgcccg agttcgaggg cgaagaggcg gcgggcgaag
     1081 agccgcagcc gagcctcttc acggactgaa ccttcccccc cccacgcgag gcaccgatga
     1141 tgagcaacga actgcaaaag cgagcccctt acaacatcag cgagccgcag tactccgtct
     1201 cgccgcagat ggtgacgacc gccttcgagc tgccgggcta ccgcgtccgg cagaacctcg
     1261 gcgtcgtgcg cggcatcgtc gtccgctcgc gcaacctctt cgttaacatc ggcgcgttct
     1321 tcaacacggt ggtcggcggc aacatcaccg cctggtcgaa cctctgcgag acgacgcgca
     1381 aggacgcctt cgacatcatg atccagcacg ccaccgaact cggcgccaac gccatcatcg
     1441 gcgcgcgcta cgacgcgacc gagatcgcca gcggcgcctc ggaagtcctc gcctacggca
     1501 cggcggtcgt cgtcgagccg ctcgaagaca cgggcatcta caactcatga gcggcgacgc
     1561 gctgaccgac gaagaactcg cgcgcatcca cgaagccttc gcgcaagtcc cctacacgcg
     1621 cctgctcggc ctggagttcg tctccgcctc gcgcggcgcc gccgtctacc gcctcaccgt
     1681 ccgcgaagag ttgacgcgca tgcgcggcat cctccacggc ggcgccgtcg cctcgctcat
     1741 ggacacggcg gcggccttcg ccgtccacac gctgctcgaa cccgcacagt ggacggtcac
     1801 ggtcgatctc acgctccact tcctccgcgc ggccagcagc ggccaactca cggcccacgc
     1861 ccgcgtcgtc cgcaacggtc ggcggctcgt catcctcaca atcgaggcga aagacgagtc
     1921 cgatcagctt atcgccatcg cttcaacgac ttacagcaaa caggtctgaa ttcactttaa
     1981 ccccgcgcta cccttttccg gcgacccgag gggccgcccg gaaggtgtgt ccgaagtgtt
     2041 aaaatcatat agacaaacat tgttggccta tgtcaaaatg ctaatgccgc tccaggctcc
     2101 cgacccggta gcgtccccaa gtcggtcttt cgccaacgcg attgtctccc cgttcccatc
     2161 tgccacccat gctgtcactc tacagcgcgc ttctcgccca gcatccccga cagctcacgc
     2221 ccgtgcgctg cgcggagcag accatcgcgc aactgcatcg ctatttcgaa gacgtggtgc
     2281 ttgagaacaa cctctcggcg ctcgtcgtcg aaggactcct gccggaggcg cagcgctctc
     2341 tgcgcgacgt ggcgcgggtg cgacaggtgg ggcgctcggc gcgctacgcc ttcttcttcg
     2401 tctcgcccga cgacggcctg accaaactcg atttgcacga gcagcggcac tccggcggcg
     2461 cgccctgcct gatgaagaag gtgacgagca cgccgttcga cgagcgcttc gtcgtcatcg
     2521 ccgacgcgcg cttctcggcg ctgctcgcgt cggtgcgcgc gcacacggag gaggggcagg
     2581 acgcgggcgg cggcgtcgag gtcatctgga cgttcgagcc ggacatcgtc tattcggcgc
     2641 tcgaatacct gatggcgcgc gtcaccgccg agcacccgtt ccaggccgcg gccttctcga
     2701 aggccgtcag cacgtcgatg ccgaagacga cctcgctcca gctcaccgtc tccgtaacga
     2761 cgaagctcgc gcgactcttg caggagcagg cgggccgaga gatcgccgtc aaccgcatcg
     2821 ccaccgccat ccgtagctcg cttgagctgc cg
//
LOCUS       DBNGOH010000268         3996 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4988178, whole genome shotgun sequence.
ACCESSION   DBNGOH010000268 DBNGOH010000000
VERSION     DBNGOH010000268.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3996)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3996)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3996
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4988178"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..352)
                     /locus_tag="AC159P_05105"
     CDS             complement(<1..352)
                     /locus_tag="AC159P_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013581919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HIR3694865.1"
                     /translation="MGTTIRAVVITVSDRCARGEQEDVSGETLAGLLREAGAEIAARL
                     ILADDLEPLAAALREQAGRADVNLVVTTGGTGLGPRDNTPEATRAVVEREVPGLSEAM
                     RAETLKQTPTAMLSR"
     gene            636..2207
                     /locus_tag="AC159P_05110"
     CDS             636..2207
                     /locus_tag="AC159P_05110"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006089956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HIR3694866.1"
                     /translation="MYDYVIVGAGSAGCVLADRLSADAGARVLLVEAGGPDRQQEVHV
                     PLAFGKLFKTACDWAYFTEEQPRMNRRRLYWPRGKMLGGSSSMNAMVYMRGHRSDYDG
                     WRAQGLEGWSYADVLPYFRKAENQERGASEHHGAGGPLNVADLRCVNPLTRAFVEAGA
                     EAGLARNEDFNGAEQEGVGLCQVTQRRGRRHSAAAAYLKPAMRRPNLHVLTHAQATRL
                     LFEGTRAVGVEFLRDERTEQVRADKEVILCGGSINSPQLLMLSGVGPAEHLRSHGLSV
                     VADLPGVGLNLQDHLFVAVAYECTRPVSLAAAESKRNILKYLLFGGGSLTSNVAEAAA
                     FVKTDAGLSAPDLQFHFGPAYYIEHGFVRPEGHGFSIGPILLRPQSRGRITLRSADPL
                     EPPSIQPDYFDADRDLRTLVEGVKLARRFAHARAFDRYRGAERYPGPAAQTDEAIADF
                     VRNTAETLYHPVGTCKMGRDREAVVDARLRVRGVEHLRVVDASVMPLIPGGNTNAPTI
                     MIAEKAADMIRSSRQ"
     gene            complement(2339..>3996)
                     /gene="carB"
                     /locus_tag="AC159P_05115"
     CDS             complement(2339..>3996)
                     /gene="carB"
                     /locus_tag="AC159P_05115"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HIR3694867.1"
                     /translation="FESFTPYLYSTYEEEDEAAPTSRRKIMILGSGPNRIGQGIEFDY
                     CCCHASFALREAGFETIMVNCNPETVSTDYDTSDRLYFEPLTFEDVMNIVDVERPEGI
                     IVQFGGQTPLNLAMRLHEAGAPIIGTSPDSIDLAEDRKRFGALLKELGIPQPEAGTAV
                     SSEEAKEVAVRVGYPVLVRPSYVLGGRAMAIVYDEKSLDEYMRTAVDASPEKPVLIDK
                     FLERAAEFDVDALADEETCVVAGIQEHIEEAGIHSGDSSCVLPPVRIAEEHLETMRHY
                     TRVMARALKVRGLLNIQYAIKDDRVYVLEVNPRASRTVPFVSKATGVPLARVAALVMA
                     GRPLSSFNLPDDLTVSRFFIKSPVFPFAKFPGVDPILSPEMHSTGEVMGVGTTFGEAY
                     AKAMTGAGLELPREGTAFISVNDADKGQAVVLARRLARLGFKLTATVGTAARLSEVGL
                     EVESVFKVNEGRPNIADKIKQGEIALVINTPLGRASHYDERAIRRAALQHNVPCVTTM
                     TGAQAVVEAIAAREHSEISVISLQELHAAEGVSGEQQAAGSRQ"
BASE COUNT          646 a         1369 c         1319 g          662 t
ORIGIN      
        1 cgcgcgagag catcgccgtc ggcgtctgct tcaacgtctc cgcgcgcatc gcctccgaga
       61 gcccgggcac ttccctctcg acgacggcgc gcgtcgcctc gggcgtgttg tcgcgcgggc
      121 cgagtcccgt cccgcccgtc gtcacgacga ggttcacgtc ggcgcgtccg gcctgctcgc
      181 gcagggcggc agcgagcggt tcgaggtcgt ccgcgagaat caggcgcgcg gcgatttcgg
      241 cgcccgcctc gcggagcagc ccggccagcg tctcgcccga cacgtcctcc tgctcgccgc
      301 gcgcgcagcg gtcgctcacc gtgatgacga ccgccctgat tgtcgtgccc atgccgcaca
      361 aagtagcttg acgaggcggc gcgcgcaagt cgtccgtcgc cgcgaatgag ctgccgattc
      421 ttgagtggac cgagcgttga tcctcaaccc agaggacacg gaggaaacac agagggcaca
      481 gagaaaacca tgagtcttct ctgtgccctc tgtgttgaaa agtccgtcac ccgagattca
      541 agatttatat agacccacgt ccgctattaa tccattatga tgaagccgct cgtatccctg
      601 tctgtgttcc aacgaagaga cggagggaag ggttgatgta cgactacgtc atcgtcgggg
      661 cgggttccgc ggggtgcgtg ctcgccgacc gcctgagcgc ggacgcgggc gcgcgcgtgc
      721 tgctcgtcga agcgggcggc cccgaccggc agcaggaggt gcacgttccc ctcgccttcg
      781 gcaagctctt caagacggcg tgcgactggg cctacttcac cgaggagcag ccgcgcatga
      841 atcgtcgccg gctctactgg ccgcgcggca agatgctcgg cgggtcgagt tcgatgaacg
      901 cgatggtcta catgcgcggg caccggagcg actacgacgg gtggcgcgcg caagggctgg
      961 aggggtggtc ttacgcggac gtgctgccct acttcaggaa ggccgagaat caggagcgcg
     1021 gcgcgtccga gcaccacggc gcgggcggcc cgctcaacgt cgccgacctg cgctgcgtca
     1081 acccgctgac gcgcgccttc gtcgaggccg gcgccgaggc gggcctcgcg cgcaacgaag
     1141 acttcaacgg cgccgagcag gaaggcgtcg gactctgcca ggtgacgcag cggcgaggcc
     1201 gtcgccacag cgccgccgcc gcctatctca agccggcgat gcgccgcccg aacctgcacg
     1261 tcctgacgca cgcgcaggcc acgcgacttc tcttcgaagg cacgcgcgcc gtcggcgtcg
     1321 agttcctgcg cgacgagcgc acagagcagg tgcgcgccga caaggaagtc atcctctgcg
     1381 gcggctcgat caactcgccg caactgctca tgctctcggg cgtcggcccc gccgaacatt
     1441 tgcgttcgca cggcctctcg gtcgtcgcag acctgcccgg cgtcggcctc aacttgcagg
     1501 atcacctctt cgtcgcggtc gcctacgagt gcacccgccc cgtctctctg gctgccgccg
     1561 agtctaagcg gaacatcctg aagtacctgc tcttcggcgg cggctcgctc acctcgaacg
     1621 tggccgaggc ggcggccttc gtcaagacag acgccgggtt gtccgcgccc gacttgcagt
     1681 tccacttcgg ccccgcctac tacatcgagc acggcttcgt ccgccccgaa ggccacggct
     1741 tctccatcgg ccccatcctg ctgcgcccgc agagccgggg tcgcatcacg ctgcgctccg
     1801 ccgacccgct tgagccgccc tccattcaac ccgactactt cgacgcggac cgagacctgc
     1861 gcacgctcgt cgaaggcgtc aagctcgccc gccgcttcgc acacgcacgc gccttcgacc
     1921 gataccgcgg cgccgaacgc taccccggcc ccgcggcgca gaccgacgag gccatcgccg
     1981 acttcgtccg caacacagcc gagacgctct accaccccgt cggcacctgc aagatgggcc
     2041 gcgaccgaga ggccgtcgtg gacgcacgcc tccgcgtccg cggcgtcgaa cacttacgcg
     2101 tcgtggacgc ctccgtcatg cccctcatcc ccggcggcaa caccaacgcc cccaccatca
     2161 tgatcgccga gaaggccgcc gacatgatac ggagcagtag gcagtaggca gcgggcagca
     2221 ggcagtgaag acggaggggc ggagaatctt gggaggtaaa gtcccgagcc tcctccctct
     2281 tcaagcaaca aagcaagagg cttgagacgc aacggctcaa gcctcttgct tttgctgcct
     2341 actgcctact gcccgctgcc tgctgctcac cgctcactcc ttcggcggcg tgaagctctt
     2401 ggagggagat gacggaaatc tctgagtgct cgcgggcggc gatggcttcg acgacggctt
     2461 gggcgccggt catggtggtg acgcagggga cgttgtgttg gagggcggcg cggcggatgg
     2521 cgcgttcgtc gtagtgggag gcgcggccca gaggggtgtt gatgacgagg gcgatctcgc
     2581 cctgtttgat tttgtcggcg atgttggggc ggccctcgtt gaccttgaag acgctctcga
     2641 cttcgaggcc gacttcgctg agtcgggcgg cggtgccgac ggtcgccgtc agcttgaagc
     2701 cgaggcgcgc gagacggcgc gcgagcacga cggcctgccc cttgtcggcg tcgttgacac
     2761 tgatgaaggc cgtgccctcg cgcggcagtt ccaaccccgc gcccgtcatc gctttggcgt
     2821 acgcctcgcc gaacgtcgtg ccgacgccca tcacctcgcc cgtcgaatgc atctcgggac
     2881 tcaagatcgg atcgacgccg gggaacttgg cgaaggggaa gacgggcgat ttgatgaaga
     2941 agcggctgac cgtgaggtcg tccggcaggt tgaagctcga gagcgggcgg cccgccatca
     3001 cgagcgcggc gacgcgcgcc agcggcaccc ccgtcgcctt cgacacgaag ggcaccgtcc
     3061 gcgacgcgcg cgggttcact tcgagcacgt agacgcggtc gtccttgatc gcgtactgga
     3121 tgttcagcag gccgcgcacc ttcagcgcgc gcgccatcac gcgtgtgtag tggcgcatcg
     3181 tctcaaggtg ctcttcggcg atgcggacgg gcggcagcac gcacgaagag tcgcccgagt
     3241 ggatgcccgc ctcttcgatg tgctcctgta tgcccgcgac gacgcaggtc tcttcgtcgg
     3301 ccagcgcatc gacatcgaac tcggcggcgc gctccagaaa cttgtcgatg agcacgggct
     3361 tttcgggcga ggcatcgacc gccgtccgca tgtactcgtc caagctcttc tcgtcgtaca
     3421 caatcgccat cgcacgcccg ccgagcacgt acgaagggcg gacgagcacc ggatagccga
     3481 cgcgcaccgc aacctctttc gcctcttcgg acgagacggc ggtgcccgcc tcgggctgcg
     3541 gtatgccgag ctccttcaag agcgcgccga agcgcttgcg gtcttccgca aggtcgatgg
     3601 agtcgggcga ggtgccgatg atgggcgcgc ccgcttcgtg caggcgcatc gccaggttga
     3661 gcggcgtctg cccgccgaac tggacgatga tgccttcggg cctttccacg tcgacgatgt
     3721 tcatcacgtc ctcgaaggtg agcggctcga agtagaggcg gtcgctggtg tcgtagtcgg
     3781 tcgagaccgt ctcggggttg cagttgacca tgatggtctc gaacccggcc tcgcgcagcg
     3841 cgaaagaggc gtggcaacag cagtagtcga actcgatgcc ctgcccgatg cggttcgggc
     3901 cggagccgag gatcatgatc ttgcggcgac tcgtcggcgc ggcctcgtcc tcttcctcgt
     3961 aggtcgagta gagatacggc gtgaaggact cgaact
//
LOCUS       DBNGOH010000269         5056 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2422951, whole genome shotgun sequence.
ACCESSION   DBNGOH010000269 DBNGOH010000000
VERSION     DBNGOH010000269.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5056)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5056)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5056
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2422951"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..670
                     /locus_tag="AC159P_05120"
     CDS             <1..670
                     /locus_tag="AC159P_05120"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020477895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIR3694868.1"
                     /translation="EALPVALRDELNDAALSILSALSKQNRPEATVAIRGALEVTDYL
                     VRRRAAELLPTGGLSSRVVTVATRNREADYRRAVARAGKTVRAVVSTDKGAFTIELLP
                     DDAPLNVDNFVELARKGFFDGITFHRVVPNFVIQGGDPRGDGNGGPGYQIRCEINQVS
                     YNRGAVGMALSGKDTGGSQWFVTHSPQPHLDGGYTVFGRVTEGLEVVDRIARGDRIRS
                     ITIR"
     gene            773..2527
                     /locus_tag="AC159P_05125"
     CDS             773..2527
                     /locus_tag="AC159P_05125"
                     /inference="COORDINATES: protein motif:HMM:NF013592.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HIR3694869.1"
                     /translation="MPFAFCLPAVGQGAAAKGRTSYRIDLKLDFEARSFSGTERVRWV
                     NREGGSTSVLYFHLYPNVRAAVAERDRSAFNGKVVPGAEQLPPPAQPPQQSAPDEPRL
                     EVTEVRAAGAGPLPFALEDGGVTLRVQLREAVGEGGAAEVEIAFKGSVPEIDADETSL
                     PAHVVQQVGAALRDTREVRRARDTNFVSRGVMLLGSFHPLLAARVRGEWRRKVEATVG
                     DTVFADVADYEVRVDAHRGVALFTSGEEVAPAGGGETPRTFAGDNLRSFSVVAGRTLR
                     SAEKTVGGVRVRSVYTAEHEKVGRRVFGVAAEAARVFTARFGALPLKTISVSEAPLVA
                     GLGSTQSSGLAVIASAFYLDFDSPQMRNLPEIVREQRASVEDSLEFAVAKSIAHQWWG
                     ASVGSDPDRAPFLDEALAHWSALLYYQEAHGPVRAKEAEDEQLRGVYQVYRTFGGEDM
                     PADRPAREYRNSFQYAAIVGAKGALMFNALRPLLGEDRFFKALRLYHDINQLKVADAE
                     DLREEFFAAVEVHQRRVVARMLARWLTERHGDEDIAAPDPQLAAALGLTLEPTGAPNR
                     NAFSRLGRFFWRQMTRIR"
     gene            2731..3252
                     /locus_tag="AC159P_05130"
     CDS             2731..3252
                     /locus_tag="AC159P_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008833927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR3694870.1"
                     /translation="MSKRVLLLVALTIGAAPTAAAQETTPHPAGDKKAAAAPAPGEMG
                     FYPPAEVKWKDGPASLSAGAKFAVLEGDPTKEGPFVMRLWFPDGFRVQPHWHPKIERV
                     TIISGTLNLGMGEKFDQSATRELTAGTFGFWPAGMRHFAWAKGDTVLQLHGTGPWVIT
                     YVNPADDPRGAKK"
     gene            3424..4248
                     /locus_tag="AC159P_05135"
     CDS             3424..4248
                     /locus_tag="AC159P_05135"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009280395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HIR3694871.1"
                     /translation="MVLLLAAGLLFGGEGQSVLTDEQSPRRWQLVWADEFDYGGLPDP
                     SKWGYDVGGHGWGNKELQFYTAGRKENARVENGHLIIEARREGYEGRGYTSARLTTRG
                     KGDWTYGRFEVAAKLPSGRGTWPAIWMLPSRKSYGDGGWPDNGEIDIMEHVGFDPDVV
                     HSSIHTRAYHHSVGTQKSARINVGSARTGFNVYAVEWTPEEVRGYVNGQHYFTFRNER
                     LANPAVDYRRWPFDRPFHLLLNVAVGGKWGGAKGIDESIWPQRMEIDYVRVYRSAP"
     gene            4659..>5056
                     /locus_tag="AC159P_05140"
     CDS             4659..>5056
                     /locus_tag="AC159P_05140"
                     /inference="COORDINATES: protein motif:HMM:NF012879.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HIR3694872.1"
                     /translation="MKRITLGLLTLLLCAATAAAQKVPVQEVTLENGMRVLMVPRKGD
                     PNIAAGWIARVGSVNERPGITGLSHLFEHMMFKGTHVVGTRDFAANQKLLDEMDAVRA
                     DLRAEEKALIRRATLGEIADAKDPQFRSER"
BASE COUNT          832 a         1754 c         1705 g          765 t
ORIGIN      
        1 cgaggcgctg cccgtcgcgc tgcgcgacga gttgaacgac gcggcgctct cgatcctctc
       61 ggcgctctcg aaacagaaca ggccggaggc gaccgtggcc atcaggggcg cgctcgaagt
      121 gaccgactac ctcgtgcgca ggcgtgccgc ggaactcttg cccacgggcg gcctctcctc
      181 gcgcgtcgtg acggtcgcca cgcgcaaccg cgaggccgac tacaggcgcg ccgtcgcgcg
      241 cgcgggcaag accgtgcgcg ccgtcgtctc gacggacaag ggcgcgttca cgatcgagct
      301 gttgcccgac gacgcgccgc tcaacgtgga caacttcgtc gagctggcgc gcaagggctt
      361 cttcgacggc atcacgttcc accgcgtcgt gccgaacttc gtcatccagg gcggcgaccc
      421 gcgcggcgac ggcaacggcg gccccggcta ccagattcgc tgcgagatca atcaggtctc
      481 ctacaaccgc ggcgccgtcg gcatggcgct ctcgggcaaa gacacgggcg gctcgcagtg
      541 gttcgtcacg cactcgccgc agccgcacct cgacggcggc tacaccgtct tcggtcgcgt
      601 cacggaagga ctcgaagtcg ttgaccgcat cgcgcgcggc gaccgcatcc gcagcatcac
      661 gatcaggtag gcgatgaagg cagaaggcaa aaggcaaaag gcaaaaggca aaagggtgga
      721 cgcgcttcgc gcgggttcga ttcttgggtc ttcgattttt gtcttttgcc ttttgccttt
      781 tgccttttgc cttcccgccg tcgggcaggg cgccgcggca aaggggcgca cgagctatcg
      841 catcgacctg aagctggact tcgaggcgcg gagcttctcg ggcacggagc gcgtgcgctg
      901 ggtgaaccgc gaaggcggga gcacgtccgt cctctacttc cacctctacc cgaacgtgcg
      961 cgccgcggtg gccgagcgcg accggtccgc cttcaacggg aaagtcgtgc ccggcgccga
     1021 gcagttgccg ccgcccgcgc agccgccgca gcagtccgcg cccgacgaac cgcgcctcga
     1081 agtgacggag gtgcgcgccg cgggcgcggg gccgctgccg ttcgcgctcg aagacggcgg
     1141 cgtgacgttg cgcgtgcagt tgcgcgaggc cgtcggcgag ggcggcgcgg cggaggtcga
     1201 gatcgccttc aaggggagcg tgcccgagat cgacgccgac gagacgagtt tgccggcgca
     1261 cgtcgtccag caggtcggcg cggcgctgcg cgacacgcgc gaggtgcgcc gcgcgcgcga
     1321 cacgaacttc gtctcgcgcg gcgtgatgct gctcggcagc ttccaccccc tgctcgcggc
     1381 gcgcgtccgc ggcgagtggc ggcgcaaggt cgaagcgacg gtcggcgaca cggtcttcgc
     1441 cgacgtggcc gactacgaag tgcgtgttga cgcacatcgc ggcgtcgcgc tcttcacctc
     1501 tggcgaagag gtcgcgcccg cgggcggggg cgagacgccg cgcacgttcg cgggcgacaa
     1561 cctgcggagc ttctcggtcg tcgcgggccg cacgctgcgc tcggcggaga agaccgtggg
     1621 cggcgtgcgc gtgcgctcgg tctacacggc ggagcacgag aaggtcgggc ggcgcgtctt
     1681 cggcgtcgcc gccgaggccg cgcgcgtctt cacggcgcgc ttcggcgcgc tccccctgaa
     1741 gacgatctcc gtgtcggagg cgccgctcgt cgcgggcctc ggcagcacgc agtcgtccgg
     1801 cctcgccgtc atcgccagcg ccttctacct cgacttcgac tcgccgcaga tgcgcaacct
     1861 gccggagatc gtgcgcgagc agcgcgcctc ggtcgaagac tctctggagt tcgccgtcgc
     1921 caagtcaatc gcgcaccagt ggtggggcgc gagcgtgggc agcgaccccg accgcgcgcc
     1981 cttcctcgac gaagcgctcg cccactggtc ggcgctgctc tactaccagg aggcgcacgg
     2041 ccccgtgcgc gccaaggagg ccgaggacga acaactgcgc ggcgtctatc aggtctaccg
     2101 caccttcggc ggcgaggaca tgcccgccga ccgccccgcg cgcgagtacc gcaactcgtt
     2161 ccagtacgcg gccatcgtcg gcgcgaaggg cgcgctcatg ttcaacgccc tgcgcccgct
     2221 gctcggcgaa gaccgcttct tcaaagcgct gcggctctac cacgacatca accagctcaa
     2281 ggtcgccgac gcggaagacc tgcgcgaaga gttcttcgcc gccgtcgaag tccaccagcg
     2341 ccgcgtcgtc gcgcgcatgc tcgcgcgctg gctcaccgaa cgccacggcg acgaggacat
     2401 cgccgcgccc gacccgcagc tcgccgccgc cctcggcctc acgctcgaac ccaccggcgc
     2461 ccccaaccgc aacgccttct cacgcctcgg cagattcttc tggcggcaga tgacgcgcat
     2521 ccggtaaagc tcgaccccct cgtcggaact gaccattcat cgaccgaacc ttcgcctccg
     2581 gccacgtcgc cgagccgggc cgcatcatcc ggcgccatcc gcgtcgggcg gccttcacgg
     2641 ccagggcgtc gagcaagcac gttgactttc tcgcgtaatc tcccatactg acacccgcgt
     2701 ccagccatcg caccggaaag gagttcgagc gtgtcgaaga gagtcctgct actcgtcgcg
     2761 ttgacaatcg gggccgcgcc gaccgcggcg gcccaggaga cgacccccca ccccgccggc
     2821 gataagaaag cggcggcggc gcccgcgccg ggcgagatgg gtttttaccc cccggcagag
     2881 gtcaagtgga aggacggccc ggcctctctg tccgccgggg cgaagttcgc ggtgctcgaa
     2941 ggcgacccga cgaaggaagg ccccttcgtg atgcgcctgt ggttcccgga cggcttccgt
     3001 gtgcagccgc actggcaccc gaagatcgag cgcgtcacga tcatctcggg gacgctcaac
     3061 ctcgggatgg gcgagaagtt cgaccagtcg gccacgcgcg agctgacagc cggcaccttc
     3121 ggcttctggc ccgcagggat gcgccacttc gcctgggcga agggcgatac cgtgctccag
     3181 ctacacggca cggggccgtg ggtcatcacc tacgtcaacc ctgcggacga cccgcgcggc
     3241 gccaagaagt aacccccgcg ggcctgagag acggggcgag ggagcttgcc tctttattcc
     3301 gcgggcactg catgaacccg gcatcgtcgc gtgggagtca acgagtcggg cggtcattcc
     3361 cgaataaaga ggctaagccg gagccgaacc gtggtggcca acccagcaca cctgccattc
     3421 cgggtggttt tacttctcgc cgccggcctg ctcttcggcg gcgaggggca gtccgtcctg
     3481 accgatgaac agagtccgcg ccgttggcaa ctcgtctggg cggacgagtt cgactacggc
     3541 ggcctgcccg acccgtcgaa gtggggctac gatgtcggcg gccacggctg gggcaacaag
     3601 gagttgcagt tttacacggc ggggcgcaag gagaacgcgc gcgtcgagaa cggccacctc
     3661 atcatcgagg cgcggcggga aggttacgag ggccgcgggt acacgtcggc ccgtttgacc
     3721 acgaggggta aaggcgactg gacgtacggt cgcttcgagg tggcggccaa gctgccctcc
     3781 gggcgcggca cctggcccgc catctggatg ctccccagtc ggaagagcta cggtgacggc
     3841 ggctggcccg acaacggcga gatcgacatc atggagcacg tcggcttcga cccggacgtg
     3901 gtgcactcct cgatccacac gagggcctac caccattcgg tcggcacgca gaagtcggcc
     3961 cggatcaatg tcgggagcgc gcgcacaggg ttcaacgtgt acgccgtcga gtggacgccc
     4021 gaggaggttc gcgggtacgt caacgggcag cactacttca ccttcaggaa tgagcgcctc
     4081 gccaacccgg cggtcgatta caggcggtgg ccgttcgaca ggccgtttca cctgctcctc
     4141 aacgtcgccg tgggcgggaa gtggggcggc gcgaaaggta tcgacgagtc gatctggccg
     4201 caacggatgg agatcgacta cgtccgggtt taccgctcgg ccccatagcc ccggcggtaa
     4261 aggtgctcgc tcgactcacc cgcattcggt agccacttcg aatcgtgggt gcgcgccgca
     4321 cggatgcgct acgtcaacct cccgctcgtg gcgagcaagc cggccaccgc ggaggagacc
     4381 gcgcacttcc tgaagctcgt gaacgacccc gcgaactggc tggtgaaggc acagccaaga
     4441 agggaagctt tcttgttcct agctgtgcct tcgtgtcttt gtggtcaata tcttccgccg
     4501 gtcttctcgt cgtttgccga gtgcatgtgc ggcgtcgtaa acttccgtgc ccggtacgcg
     4561 taaaagagct tcgtctccct ggccggggtt tcgaccggcc cgcctcgggc ggcctcccaa
     4621 cacaactgta atttcagcga gaaatcgagg aagaccaaat gaaaagaatt accctggggc
     4681 ttctcacgct cctgctgtgc gcggccaccg ccgcggcgca gaaggtgccg gtgcaggaag
     4741 tgacccttga gaacgggatg cgcgtgctga tggtgccgcg caagggcgac ccgaacatcg
     4801 ccgccggctg gatcgcgcgc gtcggctcgg tcaacgagcg gcccggcatc accggcctct
     4861 cgcacctgtt cgagcacatg atgttcaaag gcacgcacgt cgtcggcacg cgcgacttcg
     4921 cggcgaacca gaaattgctg gacgagatgg acgccgtgcg cgccgacctg cgcgccgagg
     4981 agaaggcgct catccgccgc gccactctgg gcgagatcgc cgacgccaaa gacccgcagt
     5041 tccgctccga acgcca
//
LOCUS       DBNGOH010000270         3379 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4072717, whole genome shotgun sequence.
ACCESSION   DBNGOH010000270 DBNGOH010000000
VERSION     DBNGOH010000270.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3379)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3379)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3379
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4072717"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..272
                     /locus_tag="AC159P_05145"
     CDS             <1..272
                     /locus_tag="AC159P_05145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694873.1"
                     /translation="RGEKGKRGKGEKEDEKQRACHPPSSPLPLFPLSPSLALSSLDII
                     RRVAYAGAVSSARETYKPLRGEKFFVSSVKRCSILASFVLDCSGL"
     gene            321..1376
                     /gene="recA"
                     /locus_tag="AC159P_05150"
     CDS             321..1376
                     /gene="recA"
                     /locus_tag="AC159P_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014131005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HIR3694874.1"
                     /translation="MADSKLGAERSRAIDAALANIEKKFGKGSIMRLGEREVSDVPAI
                     STTSLSLDAAIGVGGVPRGRVIEIYGPESSGKTTLALHVVAEAQKAGGVAAYIDAEHA
                     MDSEYAGKLGVSIDDLLISQPDSGEQALEITEALIRSNGLDVIVIDSVAALVPRAELD
                     GEMGDSLPGLQARLMSQALRKLTAIVAQSNTTLIFINQIREKIGVMFGSPETTTGGRA
                     LKFYSSLRLDIRRIGAIKDGDRVVGNRTKVKIVKNKVAPPFRECEFDIMYGEGISREG
                     DVLDLAVANRVVEKSGAWFSYSGERLGQGRENSKQTLKDNLELRQRIERDVRKALNMP
                     GANEATEEAEAATTAKK"
     gene            1549..2871
                     /locus_tag="AC159P_05155"
     CDS             1549..2871
                     /locus_tag="AC159P_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012283703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxyadenosine deaminase"
                     /protein_id="HIR3694875.1"
                     /translation="MAQTVIIKGGTLLTMNARGDIVEGDLLVRDGVIVEVGGRGAAAD
                     ITIDAAGCAVLPGFVQTHIHLCQTLFRGAADDLQLIDWLKKRVWPMEAAHTAASIRAS
                     ARLGIAEMMRGGTTTALTMETVSHTEEVFRAVEETGFRATVGKCMMDKGDEVPEGLRE
                     ETAASVAESLALLERWHDRAGGRIRYCFAPRFAVSCTRELLAEVGALARRHGVMVHTH
                     ASENRTEIEMVERETGMRNVAYLDSVGINGPHVLLAHCVHLDEGEMSLLSSTGTHVAH
                     CPSSNLKLGSGVAPVAELLERGVSVSLGADGAPCNNRLDMFTEMRTAALLQKVMRGPE
                     VLPAPRVLRMATIDGASALGLGAEIGSLEEGKRADVTVLDLQRLHLAPGADVVSTVVY
                     AAEASDVRDVLIDGRVVLRDGELQTLDERAVIEDARREFEALAARAGV"
     gene            3247..3319
                     /locus_tag="AC159P_05160"
     tRNA            3247..3319
                     /locus_tag="AC159P_05160"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3280..3282,aa:Phe,seq:gaa)
BASE COUNT          640 a         1071 c         1105 g          563 t
ORIGIN      
        1 gaagaggcga aaaggggaaa aggggaaaag gcgaaaagga ggatgaaaag cagcgtgcct
       61 gtcatcctcc ctcttcccct cttcccctct tccccctttc cccttccctc gctttgtcgt
      121 cgctcgacat aattcggcgc gtggcgtatg ctggcgcggt ctcgtccgcc agagaaacct
      181 acaagccact tcggggtgaa aaattttttg tttcaagtgt gaaacgctgt tccattctcg
      241 cttcatttgt gttagactgc tccggtcttt aggacacgaa ttttagaagc gggaagggac
      301 gagaaagagt gagcaccata atggctgaca gcaaattggg cgcggagcgc agcagggcga
      361 ttgacgccgc gctcgccaac atcgaaaaga aattcggcaa aggctcgatc atgcggctcg
      421 gcgagcgcga ggtgtcggac gtgccggcga tctcgaccac gtcgctctcg ctcgacgcgg
      481 ccatcggcgt cggcggcgtc ccgcgcgggc gcgtcatcga aatctacggg cctgaaagct
      541 ccggcaaaac cacgctcgcg ctccacgtcg tggccgaggc acagaaggcg ggcggcgtcg
      601 ccgcctacat cgacgccgag cacgcaatgg actccgagta cgcgggcaag ctgggcgtat
      661 cgattgacga tttgctcatc tcgcagcccg attcgggcga gcaggctctg gaaatcaccg
      721 aggcgctcat acgctcgaac ggcctggacg tgatcgtcat cgactcggtg gcggcgctcg
      781 tgccgcgcgc cgaactcgac ggcgagatgg gcgactcttt gcccggactt caggcgcgcc
      841 tgatgtcgca ggcgctcagg aagctcaccg ccatcgtcgc gcagtccaac acgaccctca
      901 tcttcatcaa ccagattcgc gagaagatcg gcgtcatgtt cggctcgccc gagacgacga
      961 ccggcgggcg cgcgctcaag ttctactcat ctttgcgcct cgacatccgc cgcatcggcg
     1021 cgatcaagga cggcgaccgc gtggtgggca accgcacgaa ggtcaagatc gtgaagaaca
     1081 aggtggcgcc gccgttccgc gagtgcgagt tcgacatcat gtacggcgag ggcatctcgc
     1141 gcgagggcga cgtgctcgac ctcgcggtgg cgaaccgcgt cgtcgaaaaa tccggcgcgt
     1201 ggttctccta ctcgggcgag cggctcgggc aggggcgcga gaactcgaag cagaccttga
     1261 aggataatct ggagctgcgc cagcgcatcg agcgcgacgt gcgcaaggcg ctcaacatgc
     1321 cgggcgcgaa cgaggcgacg gaagaggccg aggccgcgac gaccgccaag aagtaaaggc
     1381 gaccgccgtc cactttacct gtcaacaaac atcagccgcc cgcgcccgaa cctcgtcggt
     1441 agagagtccg ggcgcgggcg gctgaagctt tgccttcttt acacacacat ggcgtcgggg
     1501 cataatcccg cgcggcgcaa actccgtcac accgaagcag agaatccgat ggcgcaaact
     1561 gtcatcatca aaggcggcac gctgttgacg atgaacgcgc gcggcgacat cgtcgagggc
     1621 gacctcctcg tgcgcgacgg tgtgattgtg gaggtcggcg ggcgcggcgc cgccgcggac
     1681 atcacgattg acgcggcggg ctgcgccgtc ctgcccggct tcgtccagac tcacatacac
     1741 ctctgccaga cgctcttccg cggcgcggcg gacgacctgc aactgatcga ctggctgaag
     1801 aaacgcgtct ggccgatgga ggccgcgcac acggcggcgt cgatccgcgc ctccgcgcgc
     1861 ctcggcatcg ccgagatgat gcgcggcggc acgaccacgg cgctgacgat ggagaccgtg
     1921 agccacaccg aagaggtctt ccgcgcggtc gaggagactg gcttccgcgc gacagtcggc
     1981 aagtgcatga tggacaaggg cgatgaagtg cccgaaggct tgcgcgaaga gaccgccgcg
     2041 tccgtcgccg aatcgctggc gctgctcgaa cgctggcacg accgcgcggg ggggcggatc
     2101 cgctactgct tcgcgccgcg cttcgccgtc agttgcacgc gcgagctgct ggcggaggtc
     2161 ggcgcgctcg cgcggcgcca cggcgtgatg gtgcacacgc acgcctccga gaatcgaacg
     2221 gagatcgaga tggtcgagcg cgagacgggc atgcgcaacg tcgcgtacct cgattcggtc
     2281 ggcatcaacg gtccgcacgt cctgctcgcg cactgcgtcc acctcgacga gggcgagatg
     2341 agcctgctct cttcgaccgg cacgcacgtc gcgcactgcc cttcgtcgaa cctcaagctc
     2401 ggctccggcg tcgcgccggt cgcggagttg ctggagcgcg gcgtctcggt ctcgctcggc
     2461 gccgacggcg cgccctgcaa caaccgcctc gacatgttca cggagatgcg caccgccgcg
     2521 ctgcttcaga aggtgatgcg cgggccggag gtcttgcccg cgccgcgcgt gctgcgaatg
     2581 gcgaccatcg acggcgcaag cgcgctcgga ctcggcgccg agatcggcag cctcgaagaa
     2641 gggaagcgcg ccgacgtgac tgtgctcgac ctgcaaaggc tccaccttgc gccgggggcc
     2701 gacgtcgtct cgaccgttgt ctacgccgcc gaagcgagcg acgtgcgcga cgtgttgata
     2761 gacgggcgcg tcgtgctccg cgacggcgag ttacagacgt tggacgagcg cgccgtcatc
     2821 gaagacgcgc gcagagagtt cgaagcactc gccgcaaggg cgggcgtcta gccgcgcatt
     2881 cggggcctca gccgcggagt tacaggacgg gagtggctga attatcagtg gttggacggg
     2941 gcgggccgac ttcgggatag aaaaatgcca gctcgaaggt agcggctgtg gggggcacaa
     3001 tcggcgttcg agctggcact ggcgggcact ttcaagttcg ggcttgggat ggcttgtcct
     3061 tttcagcacc gccggctgtt tggcaaccgg caccaccctc aacgccggga attatacacc
     3121 tcctcacaaa actgtcaacg tttttttgtg accgatttga caaaaaacag ggtcgcccga
     3181 aacccggttg acagccctgt ttcgaccccc ttatactttc ccacctgcct gcacaaagac
     3241 atttccggcc aggtagctca gttggtagag cacgtgactg aaaatcacgg tgtcggcggt
     3301 tcgattccgt ccctggccat cgaacaaaaa taaaagcgag acgggcggct attgaggccg
     3361 cccgtttcgc tttctctgc
//
LOCUS       DBNGOH010000271         2375 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4255089, whole genome shotgun sequence.
ACCESSION   DBNGOH010000271 DBNGOH010000000
VERSION     DBNGOH010000271.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2375)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2375)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2375
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4255089"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..269
                     /locus_tag="AC159P_05165"
     CDS             <1..269
                     /locus_tag="AC159P_05165"
                     /inference="COORDINATES: protein motif:HMM:NF024180.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HIR3694876.1"
                     /translation="LVEKRSPAAALKGAQTAVWQQRRWRAPYFWAAFVLQGEYGGHVE
                     VGGAAPPVVTYRRAGAAAGAALALLVCGGYFIRRKARHGRTHVH"
     gene            259..870
                     /locus_tag="AC159P_05170"
     CDS             259..870
                     /locus_tag="AC159P_05170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694877.1"
                     /translation="MCTDSQTRSAGDGKWVLDEAAFERLLAWLDPERERAGQRYEDIR
                     VRLIKIFTCRGCHESEDLADETINRVARKLAEVAATYEGDRALYFYGVAHRVYLEYLR
                     KPRAHAAAAPLPAPPEPTEEERAADEREYECLERCMGGLPAASRDLVLQYYREEKREK
                     IEHRKRLAARLGIGLNALRIRACRIRETLYQCVQTCLGEGAAG"
     gene            1097..2008
                     /locus_tag="AC159P_05175"
     CDS             1097..2008
                     /locus_tag="AC159P_05175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694878.1"
                     /translation="MEEYKNNGALVRLYLLGELAEEEQRRFEEELMAGDRVFEELLAA
                     EDELIDQYVEGMLTARERERFERHFLAAPERRRKLGFARTLRKYIADGWAEEVSVEGA
                     TSDASSVEAASTEEASEEESADGEPTSARGGGPRPPSWERLLPPFLRPRQPLARYAFA
                     AVLLLALSAGALLVFDALRGPQRRGAVVAFTLAPGGGVRDAGGAGANRLRVPADAPAV
                     ELRLPLATLEHRSYRAVIENVNEDGGVFESDELPPPAAGAERIVVVRVPANDLAPGDY
                     KLKLRGANAAGQFEDAATYYFRVTEGK"
     gene            complement(2175..>2375)
                     /locus_tag="AC159P_05180"
     CDS             complement(2175..>2375)
                     /locus_tag="AC159P_05180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694879.1"
                     /translation="QILSDARRQFREARRDSEPRMEDIRRQTDERLRQVLTPEQFQRM
                     KEEMGKRRRGRRAREGEKESRQ"
BASE COUNT          383 a          822 c          809 g          361 t
ORIGIN      
        1 tgctcgtcga gaagagatcg ccggcggcgg cgctcaaggg ggcgcagacc gccgtgtggc
       61 agcagcggcg ctggcgcgcg ccctacttct gggcggcctt cgtgcttcag ggcgagtacg
      121 gtggccacgt cgaagtgggc ggggccgcgc cgcccgtcgt cacctaccgg cgcgcgggag
      181 ccgcggcggg cgcggcgctc gcccttctcg tgtgcggggg ctacttcatc aggaggaagg
      241 cgcgacacgg aagaacccat gtgcactgac tctcagacgc gttcggcggg cgacggcaag
      301 tgggttctgg acgaggcggc cttcgaaagg ctgctcgcct ggctcgaccc cgagcgcgag
      361 cgggccgggc aacggtacga ggacatccgc gtccgcctca tcaaaatctt cacctgccgt
      421 gggtgccacg agtcggaaga cctcgccgac gagacgatca accgcgtcgc ccgcaagctc
      481 gcagaggtcg ccgcgacgta cgagggcgac cgggcgctct acttctacgg cgtggcccac
      541 agggtctacc tcgaatacct gcggaagccg cgcgcgcacg ccgccgccgc ccctctgccg
      601 gcgccgcccg aaccgacgga ggaggagcgg gccgcggacg agcgcgagta cgaatgcctt
      661 gagcgctgca tgggcgggct gcccgccgcg agccgcgacc tcgtgctgca atactaccgc
      721 gaggagaagc gcgagaagat cgagcaccgg aagcgcctgg ccgcgcggct cggcatcggc
      781 ctcaacgcgc tccgcatccg cgcctgccgc atccgcgaga cgctctacca gtgcgtgcag
      841 acctgcctcg gcgaaggggc cgccgggtga aacgtccccc acgtgaaatg tattccgcgg
      901 attcgcataa ccgtttgaag ggtcttttcg gagcgaccgt gcgagaggcc gggcggggcg
      961 tgagccgaac cgcgcggagg gagccttttg cgccgccgcc gcctcgccgc ccttcctcac
     1021 gtgccagctc acgccccagg ccgttcgccc ccccgatctc tccctgtgtc cgtgcgaccg
     1081 tggaagaacc gcgctgatgg aagagtataa aaacaacggg gcgttggtca ggctgtacct
     1141 gctgggcgaa ctcgccgagg aggagcaacg gcgattcgaa gaggagctta tggccggcga
     1201 ccgcgtcttc gaagaactgc tcgccgcgga ggacgagttg atcgaccagt acgtcgaagg
     1261 catgctcacg gcgcgcgagc gcgagcggtt cgaacggcac ttcctcgccg cccccgaacg
     1321 gcgccgcaag ctcggcttcg cccgcacgct cagaaaatat atcgcggacg gctgggccga
     1381 agaggtctcg gtcgagggtg cgacctccga cgcctcttcg gtcgaagccg cttcgaccga
     1441 agaggcgtcg gaggaagagt ccgccgacgg agagcctaca agcgcccgcg gcggcgggcc
     1501 gcgcccccct tcgtgggagc gcctgctccc gccgttcctc cgcccgcggc agccgctcgc
     1561 gcgctacgcc ttcgccgccg tcctgctgct cgcgctctcg gcgggcgcgc tgctcgtctt
     1621 cgacgcgctg cgagggccgc agcggcgggg cgccgtcgtc gccttcacgc tggcgcccgg
     1681 cggcggcgtc agggatgcgg gcggcgcggg cgccaacagg ctgcgcgtcc ccgcggacgc
     1741 gcccgccgtg gagctacggc tcccgctcgc gacgctcgaa cacaggagct accgcgccgt
     1801 catcgagaac gtgaacgaag acggcggcgt cttcgagtcg gacgagctgc cgccgcccgc
     1861 ggcgggcgcc gaacgaatcg tcgtcgtccg cgtccccgca aacgacctcg cccccggcga
     1921 ctacaaactc aaactccgcg gcgccaacgc cgccggccag ttcgaggacg ccgccaccta
     1981 ttacttccgc gtcacggaag ggaagtaggc ggtaggcagc aggcagtagg cagtaaaaga
     2041 cagaagaaga aaaccgaaga agcaagaggc ttgagatggg atgtttcaag cctcttggtt
     2101 cgatatgcag taaataaaga ggcttgagat tcaatatctc aagcctcttt atctttgttc
     2161 tttcgccttg ctgcctactg cctactttcc ttctcgcctt cccgcgcccg cctgccgcgt
     2221 ctgcgcttgc ccatctcttc cttcatccgc tggaactgtt cgggcgtgag cacctgtcgc
     2281 aggcgctcgt cggtctgccg gcggatgtct tccatgcgcg gctcggagtc gcggcgggct
     2341 tcgcggaact gcctgcgcgc gtcggagagt atctg
//
LOCUS       DBNGOH010000272         5188 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_772846, whole genome shotgun sequence.
ACCESSION   DBNGOH010000272 DBNGOH010000000
VERSION     DBNGOH010000272.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5188)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5188)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5188
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_772846"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3688)
                     /locus_tag="AC159P_05185"
     CDS             complement(<1..3688)
                     /locus_tag="AC159P_05185"
                     /inference="COORDINATES: protein motif:HMM:NF051698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR3694880.1"
                     /translation="MLSLGGAAAQSGTSTLRGTVVDQQGNAVTGASVTLSNTEKNVIR
                     TQTTNADGGFVFSSVPPGVYRVEVEAAGFKKALAADVRAVVDTPVDVNVQLEAGNITE
                     VVSVSAGLDAPINTTDATIGNTFESRRIEELPLNARNIVGLLSLQPGVTSGGAVNGGR
                     ADQSNITLDGVDVNEQQAGLDVVATTVTGINQAFGSVLRVTPDSVQEFRVVTTNPNAE
                     QGRSSGAQVSLVTKSGTNQLHGSLYHFHRNTVTTANDFFNNKAGTFGPNDPQVLSGTA
                     VVGDPRVPRPPLLRNIFGGSVGGPIKKDRAYFFFTYEGFREATSTSAVKVVPLASTIG
                     QGIIRYRSASGASDPSCPAGTPAGFRCLTPAQIDAAYLAANGVSPGTNALAINYLANI
                     ARRYPANDTTVGDGINTGGFRFNARTPTNQDTFIGKLDFNLTSRQTMFVRGNYQDDLV
                     KLAPDLPDTVPPQIWYHPKGLAVGHTWVVTNRLVNKVTYGFTRAAFSQQGESTDPNVS
                     FRFIFQPGTVAGIAPARTLTRVTPVHNIVDDLSWSRGNHTIQFGGNLRFIENRRSSFT
                     SSYDFATTNPSGYEASSAVLTTSVNNTPIFADVAQSSVTPLRNALAAFIGRFSQYNAS
                     IQYDPEGNILPPGSPADRTFATEEYELYGQDSWRIRPNFTLNLGLRWSTTTPVYEANG
                     FQVQPVQSLGEFFERRIEGMETGVPYNELLTLDRSGKANDRPGFYKQDWNNFGPSVSF
                     AWSPDFGDNFLGRLVGRDGKSVLRGGFRMTFDRVGSQLAVSFDLNNSLGFNSAPSTSA
                     GQFNVTDALPPLFTTLTPNVRTLPFIAGTFDERLIFPLTEPADNSERIQASLDDSITT
                     PYNYSVNLSYAREIGKGLSFEASFVGRYARDLLAQRDVMHFNNIRDPRSGQNWYDAIR
                     QLIDHRYAATPITSVPAIPFFENLLPGIAGTFAVNGVNTPLTATQSAYRRIALASVGG
                     QNVTDYTFLQSNLRWNNRPFSIFDNTFVHPQYATLMVWSTIAKSNYNAGQFSLRQRFR
                     NDLTFDLNYTLSNSHDNASGLQNAGFGSTTALIFDPTNVDSNYANSDFDVRHIINANW
                     LYGLPIGRGKAFLSDLPRVANAVLGGWQLTGIFRWNSGLPTAAFRPFAFQRWATNWQI
                     SSGMVATRPVTTTSGDVNGEPNIFADPQAAFLSYRDPLPGEPGDRNKLRYPGYVSLDA
                     ALHKTFHMPWEGHRVTFRWEVFNVT"
     gene            complement(4193..4453)
                     /locus_tag="AC159P_05190"
                     /pseudo
     CDS             complement(4193..4453)
                     /locus_tag="AC159P_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_082110585.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(4653..>5188)
                     /locus_tag="AC159P_05195"
     CDS             complement(4653..>5188)
                     /locus_tag="AC159P_05195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694881.1"
                     /translation="TAPDPRPTVSRPRRQDVGPGSVPRQRTTLRVEAPVEEVVSRLRQ
                     ELEPSLRAAARRAAAREHERTREWLESRGLPKAARVAQREAYNVLRKYGVIRDTIQSA
                     HADVGRSCLVQEPRTLSDGEVEELAQACVAMLEVKGQSIELTLSEMSVEAGGFLLPED
                     APRVRARAEALLQDSRR"
BASE COUNT          818 a         1537 c         1903 g          930 t
ORIGIN      
        1 tggtgacgtt gaagacctcc caacggaacg tcacgcggtg tccctcccag ggcatgtgga
       61 aggtcttgtg cagggccgcg tcgagcgaga cgtagccggg gtagcgcagc ttgttgcggt
      121 cgcccggctc gcccggcagc gggtcgcggt agctgaggaa agcggcctgc gggtcggcga
      181 agatgttcgg ctcgccgttg acatcgccgg acgtggtggt gacgggccgc gtggcgacca
      241 tccccgaaga gatctgccag ttggtggccc agcgctggaa ggcgaagggc cggaaggccg
      301 ccgtcggcag gcccgagttc caacggaaga tgccggtcaa ctgccagccg ccgagcaccg
      361 cgttggccac gcgcggcagg tcgctcaaga acgccttgcc gcgcccgatg ggcaggccgt
      421 agagccagtt ggcgttgatg atgtggcgca cgtcgaagtc cgagttggcg tagttgctgt
      481 ctacgttggt cgggtcgaag atgagcgccg tggtcgagcc gaagcctgcg ttctggaggc
      541 ccgaggcgtt gtcgtgcgag ttcgagagcg tgtagttgag gtcgaaggtg aggtcgttgc
      601 ggaacctctg gcgcagggag aactgcccgg cgttgtagtt cgacttcgcg atcgtggacc
      661 agaccatgag cgtcgcgtac tgcgggtgga cgaacgtgtt gtcgaagatc gagaagggcc
      721 ggttgttcca gcgcaggttc gactggagga aggtgtagtc ggtgacgttc tgcccgccga
      781 cgctggcgag cgcgatcctg cggtaggcgc tctgcgtcgc cgtgagcggc gtgttgacgc
      841 cgttgacggc gaaggtgccg gcgatgccgg gcaggaggtt ctcgaagaac gggatggcgg
      901 gcaccgaggt gatcggcgtc gccgcgtagc ggtggtcgat gagctggcgg atggcgtcgt
      961 accaattctg gcccgagcgc gggtcgcgga tgttgttgaa gtgcatcacg tcgcgctgcg
     1021 ccagcaggtc gcgcgcgtag cgcccgacga agctcgcctc gaacgagagg cccttgccga
     1081 tctcgcgcgc ataggagagg ttgacgctgt agttgtaggg cgtcgtgatc gagtcgtcga
     1141 gcgacgcctg gatgcgctcg ctgttatccg ccggctcggt cagcgggaag atcagtcgtt
     1201 cgtcgaaggt gccggcgatg aacggcaggg tgcggacgtt cggcgtcagc gtggtgaaga
     1261 gcggcggcag ggcgtcggtg acgttgaact ggccggcgga ggtggagggt gccgagttga
     1321 acccgagcga gttgttgagg tcgaaggaga cggcgagctg gctccccaca cggtcgaagg
     1381 tcatgcggaa gccgccgcgc aggacgctct tgccgtcgcg cccgaccaga cggcccagga
     1441 agttgtcgcc gaagtcgggc gaccacgcga acgagaccga agggccgaag ttgttccagt
     1501 cctgtttgta gaagccgggc cggtcgttgg ccttgcccga acggtcgagc gtgagcaact
     1561 cgttgtaggg cacgcccgtc tccatcccct cgatgcgccg ctcgaagaat tcgccgaggc
     1621 tctgcacggg ttgcacctgg aagccgttgg cctcgtagac gggcgtggtg gttgaccagc
     1681 gcagcccgag gttgagcgtg aagttggggc ggatgcgcca cgagtcctgc ccgtagagtt
     1741 cgtactcctc ggtagcgaag gtgcggtcgg caggcgagcc gggcgggagg atgttgcctt
     1801 cggggtcgta ctggatgctc gcgttgtact gcgagaagcg accgatgaag gcggccagcg
     1861 cgttacgcag cggggtgacc gaactctggg ccacgtccgc gaagatcggc gtgttgttga
     1921 cgctcgtggt caagaccgcg gacgacgcct cgtaaccgga agggttggtc gtggcgaagt
     1981 cgtaggagga cgtgaacgag ctgcgtcggt tctcgatgaa gcgcaggttg ccgccgaact
     2041 ggatggtgtg gttgcccctg ctccacgaca ggtcgtccac gatgttgtgg acgggcgtga
     2101 cgcgcgtcag cgtgcgcgcc ggcgcgatcc cggcgaccgt ccccggctgg aagatgaaac
     2161 ggaagctgac gttcgggtcg gtgctttcgc cctgctggct gaaggccgcg cgggtgaagc
     2221 cgtaggtgac tttgttgacg agccggttgg tgacgaccca cgtgtggccg acggccagac
     2281 ccttcgggtg ataccagatc tgcggcggga cggtgtcggg caggtcgggc gcgagcttga
     2341 cgaggtcgtc ctgatagttg ccgcgcacga acatggtctg gcggctcgtc aggttgaagt
     2401 cgagtttgcc gatgaaggtg tcctggttgg tcggcgtgcg cgcgttgaag cggaagccgc
     2461 cggtattgat gccgtcgccg accgtcgtgt cgttggccgg gtagcggcgc gcgatgttgg
     2521 ccaggtagtt gatggccagc gcgttcgtgc cggggctgac cccgttggcg gcgaggtagg
     2581 cggcgtcgat ctgcgcgggt gtcaggcagc ggaagcccgc gggcgtgccc gcagggcacg
     2641 aggggtcgct cgccccgctc gccgaacggt agcggatgat gccctgcccg atggttgacg
     2701 cgagcggcac gaccttgacg gcgctcgtcg aggtcgcctc gcggaagccc tcgtaggtga
     2761 agaagaagta cgcgcggtct ttcttgatag ggccgccgac ggagccgccg aagatgttgc
     2821 gcaggagcgg cgggcgcggc acgcgcgggt cgccgacgac ggcggtgccg gagagcacct
     2881 gcgggtcgtt cgggccgaag gtgcccgcct tgttgttgaa gaagtcgttg gcggtcgtca
     2941 ccgtgttgcg gtggaagtgg tagagcgagc cgtgcaactg gttcgtgccc gacttggtga
     3001 cgagcgagac ctgcgcgccg gaggagcgcc cctgctcggc gttcgggttg gtcgtcacga
     3061 cgcggaactc ctgcaccgag tcgggcgtga cgcgcaagac gctgccgaac gcctggttga
     3121 tgcccgtcac cgtcgtcgcg accacgtcga ggccggcctg ctgttcgttc acgtccacgc
     3181 cgtcgagcgt gatgttcgac tggtctgcgc gcccgccgtt cacggccccg ccgctcgtta
     3241 cgccgggttg caggctgagc agcccgacga tgttgcgcgc gttcaagggc agctcctcga
     3301 tgcgccggct ctcgaaggtg ttgccgatgg tcgcgtccgt cgtgttgatc ggcgcgtcca
     3361 gcccggcgct gacgctcacg acctctgtga tgttgcctgc ctcaagctgc acgttgacat
     3421 cgacgggcgt atcgacgacc gcacgcacgt ccgccgccag cgccttcttg aagcccgccg
     3481 cctcgacctc gacccggtag acgcccggcg ggacggagct gaagacgaac ccgccgtccg
     3541 cgttcgtcgt ctgggtgcgg atgacgttct tctccgtgtt ggagagcgtc acgctcgcgc
     3601 ccgtgacggc gttgccctgc tggtcaacta ccgttccccg taaagtggac gtgccgctct
     3661 gcgcggcggc gccgccgagg gacaacatgg agaggattag gacgctcgcg agagccgcga
     3721 accgacttgc aaagagactg tgccgtatca tcatgacctc cgttgtgatt tggaaaggct
     3781 gtcaacccgg caggcgcgac acggggttgt tccgacgccc ctgtccacgt ccgagcctta
     3841 ggcgtgtgcg ctgaccctgt ccgcacggac tcaccgcctc tgtgcggcgc gcgcacttgc
     3901 ggcgcgaggt gtgcgccgcc cgaagccgtg cgaaatattg gacaggagaa cgaccgtcga
     3961 tgcctgaccg ggacaggtag atgcaaagga cgtaccgaca acgcgcgccg agaggttacg
     4021 cgagcttact gacgaaattt aacggcgagc tactatcgga gacggcccgg cgcattgcca
     4081 tcttcgtaat gttattgaga gatttggaga cctgtggtga gcgcgaccgg attggggggt
     4141 gcgttggtac gattatttgg actgccgaac tgtcggagac cgacgacgga cattattgac
     4201 acgcgagttc gacgtgagcg aaccggaagg caaactgcaa ccgctctctg agacgaatgt
     4261 tcaagccggg ctgcttctcg cgccacgtgt aggcgacgag acctgcgaag aggttgacga
     4321 agcggttcgc caccgagcgg tggcggctgt gctcgatccg cgagaggtgc ttgagccggt
     4381 cagccacgct ctcgatgacg gtcagcttgc gcagcacgct ccggtcgagg agcggcatca
     4441 gccggttttt catccacgct tgagcttcgt ctcgtcgagt ccacgaacga gacgccgcag
     4501 cagcggcctt tgcgactgtg caggtagccg cacaagggga cgagtgcggg gcgggcatca
     4561 gttccacgaa gcggctgtag ctgacgaggc gcgggatggc ccatgccagg cgcttcggga
     4621 cgagccacgt gtagaaaacc ttccgacggt tattaccgcc tcgaatcttg aagcagggcc
     4681 tcggccctcg cgcgtacgcg aggggcgtct tcgggcagca ggaagccccc cgcctcgacg
     4741 ctcatctccg acagcgttag ttcaatcgac tgccccttga cttcgagcat ggcgacgcag
     4801 gcttgggcca gttcttcgac ctcgccgtcc gaaagcgtcc gaggctcttg gacgaggcaa
     4861 ctgcggccca catccgcgtg cgcggactgg atcgtgtccc tgatgactcc gtacttgcgg
     4921 agcacgttgt aggcctcgcg ctgtgcgact ctcgccgcct tcggcagtcc cctgctctcg
     4981 agccactcgc gcgtccgctc gtgctcccgc gccgccgctc gacgcgccgc cgctcggagc
     5041 gaaggctcga gctcctgcct cagccgcgag acgacctcct cgaccggggc ctcgacccgc
     5101 agcgttgtgc gttgtcgggg cacactcccg gggccgacat cctgccggcg aggtcgggag
     5161 actgtcgggc gcgggtcggg ggctgtgt
//
LOCUS       DBNGOH010000273         2045 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1323733, whole genome shotgun sequence.
ACCESSION   DBNGOH010000273 DBNGOH010000000
VERSION     DBNGOH010000273.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2045)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2045)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2045
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1323733"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2045)
                     /gene="bglX"
                     /locus_tag="AC159P_05200"
     CDS             complement(<1..>2045)
                     /gene="bglX"
                     /locus_tag="AC159P_05200"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HIR3694882.1"
                     /translation="MTLEEKLGQLQQLDGHADGRFREEHVELTRKGLLGSTLNVRGAK
                     QSNELQRVAVEQSRLKIPLIFGFDVIHGYRTIFPIPLGEAASWDAAAVERAAAVAAKE
                     AKSAGVHWTFAPMVDIARDARWGRIAEGSGEDPYLGSALARARVRGFQGTDYSAPDRV
                     VACAKHWVAYGAAEAGRDYNTTDMSERTLREVYLPPFKAAVDAGVGTFMSAFNDLNGV
                     PTSGNHFTLTKILRGEWKFDGFVVSDYESVREMLNHGYAANEAEAARLGLSAGVDMEM
                     VSRLYNKHGASLLSGRGLTQAEIDEAVRRILRVKFRAGLFEKPYADEARERAAILNAE
                     HLAAAREVAARSMVLLKNERGTLPLSKTVGTVAVVGPLSDDPKAVLGSWAGDGRSEDA
                     VSLLAGIKAKVSPQTRVLHAKGVAIDVRGATGNYDAPPPQASNAGGTNVASAANTEVA
                     RLATTPVGPDSIAEAVRVAREADVVVVAVGETAEMSGEAASRTSLDLPGRQPELLQAI
                     QETGKPYAVVLMNGRPLSINWLAENSPAILETWFAGTQAGHAIADVLFGDVNPGGKLP
                     VTFPRHAGQSPIYYNYKTTGRPPTDQKYTSKYLDMPWTPLYPFGHGLSYTTFRLSGLQ
                     LSSQRVAPDGRLTASVEVENTGSRAGDEVVQLYVRDVAASVTRPVRELKGFERVTLRP
                     "
BASE COUNT          281 a          690 c          732 g          342 t
ORIGIN      
        1 gggcgcaacg tgacgcgctc gaagcccttc agctcgcgca cagggcgcgt gacgcttgcg
       61 gccacgtcgc gcacgtagag ctgcacgacc tcgtcgcccg cgcggctgcc tgtgttctcg
      121 acttcgacgc tggcggtcag gcgaccgtcc ggcgcgacgc gttgcgaaga gagttgcagg
      181 ccggagaggc ggaacgtcgt gtagctcagg ccgtggccga acgggtagag cggcgtccac
      241 ggcatgtcca gatatttcga ggtgtacttc tggtcggtcg gcgggcggcc cgtcgtcttg
      301 tagttgtagt agatgggcga ttgccctgcg tggcgcggga aggtgacggg gagcttgccg
      361 ccggggttca cgtcgccgaa gagcacgtcg gcgatggcgt ggccggcctg cgtgccggcg
      421 aaccacgttt cgaggatggc gggcgagttc tccgcgagcc agttgatgga gaggggccgc
      481 ccgttcatca ggacgacggc gtatggcttg cccgtctctt gaatcgcttg cagcaactcc
      541 ggctgcctgc ccggcaagtc gagcgacgtg cgcgaagcgg cctcgccgct catctctgcg
      601 gtctcgccga cggcgacgac gaccacgtcc gcctcgcgcg cgacgcgcac ggcctccgct
      661 atcgagtcgg ggccgaccgg cgtggtcgca aggcgcgcga cctccgtgtt cgcggcggag
      721 gcgacgttgg tgccgcccgc gttgctcgcc tgcggcggcg gcgcgtcgta gttgccggtc
      781 gcgccgcgca cgtcaatcgc cacgcccttc gcgtgcagca cgcgcgtctg cggcgagacc
      841 ttcgccttta tccctgcgag caggctgacc gcgtcttccg agcgaccgtc gcccgcccaa
      901 gagccgagca ccgccttcgg gtcgtccgag agcgggccga cgacggcgac cgtgccgacg
      961 gtcttgctga gcggcaaagt cccgcgctcg ttcttcaaga gcaccatcga gcgcgcggcc
     1021 acttcgcgcg ccgccgccag atgctcggcg ttgaggatgg ccgcgcgttc gcgcgcctcg
     1081 tccgcgtaag gtttctcgaa gaggccggcg cggaatttga cgcggaggat gcggcgcaca
     1141 gcctcgtcga tctcggcctg cgtgagtccg cgcccggaaa gaagggaagc gccgtgcttg
     1201 ttgtagaggc ggctgaccat ctccatgtcc acgcccgccg aaaggccgag gcgcgcggcc
     1261 tccgcttcgt tcgccgcgta gccgtggttc aacatctcgc gcaccgactc gtagtcgctg
     1321 acgacgaagc cgtcgaactt ccattcgccg cgcaggattt tcgtgagcgt gaaatgattg
     1381 ccggaagtgg gcacgccgtt gaggtcgttg aaggcgctca tgaaggtgcc gacgcccgcg
     1441 tcaacggcgg ccttgaaggg cggcaggtag acttcgcgca gcgtgcgctc gctcatgtcg
     1501 gtcgtgttgt agtcgcggcc cgcttccgcc gcgccgtagg cgacccagtg cttggcgcag
     1561 gcgacgacgc ggtcgggcgc gctgtagtcc gtgccctgaa agccgcgcac gcgcgcgcgc
     1621 gcgagcgccg agccgagata cgggtcttcg cccgagcctt cggcgatgcg gccccagcgc
     1681 gcgtcgcgcg cgatgtccac catcggcgcg aacgtccagt ggacgcccgc cgacttggcc
     1741 tccttcgcgg cgaccgccgc cgcgcgctcg accgccgccg cgtcccagct cgcggcctcg
     1801 ccgagcggga tggggaagat cgtgcggtag ccgtggatca cgtcgaagcc gaagatgagc
     1861 ggaatcttga ggcgcgactg ctcgacggcg acgcgctgaa gctcgttcga ttgcttcgcg
     1921 ccgcggacgt tcagggtcga gccgagcagc cctttgcgcg tcagctcgac gtgctcctcg
     1981 cggaagcgac cgtcggcgtg gccgtcgagc tgttgcagtt gcccgagctt ctcttcgagc
     2041 gtcat
//
LOCUS       DBNGOH010000274         2186 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4255299, whole genome shotgun sequence.
ACCESSION   DBNGOH010000274 DBNGOH010000000
VERSION     DBNGOH010000274.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2186)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2186)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2186
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4255299"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2186)
                     /locus_tag="AC159P_05205"
     CDS             complement(<1..>2186)
                     /locus_tag="AC159P_05205"
                     /inference="COORDINATES: protein motif:HMM:NF014156.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NPCBM/NEW2 domain-containing protein"
                     /protein_id="HIR3694883.1"
                     /translation="SRFALPPAGWCSWYFYYQEIGEDEVKRNARWIAEHLKDYGAGLV
                     QIDDGWQGVGRGQGENRDWTTIDKRFPGGMPHLAAYIKSLGLTPGIWLAPHGQSNEAL
                     VRSNPGAFMLKPDGTSVVSTWEGRFLVDPSTPEGQRYLKDLFTTLSGWGYEYFKIDGQ
                     PIVIREYRNRKAAMRNPPTDADKLYRDTLESIRAGIGPGRYLLGCWVIPLEGVGLMDG
                     SRTGADILPNWDGFKFALRATMEYYFLHNVAWYADPDVMVVRAPLPLEQARAWATLQG
                     LTGQAVLTSDRLMDLSPERVELLRRVMPAADVRPLDLFKSERNKRVWDLKVNHLGRRY
                     DVVGLFNFDERGQAPLYVGWKDLGLPEGARVQVFDFWNKEYLGEWEQGLTIDLAPASS
                     RVLTLMPASGSVQLISTSRHITQGWVDLVAHRYDAARGSYAGRSKVIKNDPYELRFSF
                     PRGKNFVVKKATARGASGLLPVKISNHDGWAAVEFTSPRTAEVGWEVVFEPSSHYRVP
                     VREPQSPWAEGAGVDGVNLRWGMPHQPAVGYQVSLNGEPRGFTPTQVFALRGLDMNAA
                     HTAEVRTVWHDGTTSERAAQIKFTPRELLPQGLALSDLDPSRVSHGWRQPELNRTHTG
                     GGLSIKGRHYASGVGMPTNSEIEFEVRAAYETFDALVGVDDEERSADGAVEFFLVGDG
                     RELWRSGPLKKADGAKPVKVGIKGVERLTLRVARVGEGGRLHADWV"
BASE COUNT          324 a          779 c          714 g          369 t
ORIGIN      
        1 cacccagtcg gcgtggaggc gaccgccttc gccgacgcgt gcgacgcgga gcgtgagtcg
       61 ctcgacgccc ttgatgccga ccttcaccgg cttcgcgccg tcggccttct tgagagggcc
      121 gctgcgccac aactccctgc cgtcgccgac gaggaagaac tcgaccgcgc cgtcggcgct
      181 gcgctcctcg tcgtccacgc cgacgagcgc gtcgaaggtc tcgtatgcgg cgcgcacctc
      241 gaactcgatc tcggagttgg tcggcatgcc gacgccgctc gcgtagtgcc tgcccttgat
      301 cgagaggccg ccgcccgtgt gcgtgcggtt cagctcgggc tgccgccagc cgtgcgagac
      361 gcgcgagggg tcgaggtcag agagcgccag cccctgcggc agcagctccc tcggcgtgaa
      421 ctttatttgc gccgcccttt cgctcgtcgt cccgtcgtgc cagacggttc tgacctccgc
      481 cgtgtgcgcc gcgttcatgt cgaggccgcg caaggcgaag acctgcgtcg gcgtgaagcc
      541 gcgcggctcc ccgttcaagg agacctgata gccgaccgcg ggctggtgcg gcattcccca
      601 acgcaggttc acgccgtcca cgcccgcgcc ttcggcccac ggactttgcg gctcgcgcac
      661 ggggacgcgg tagtggctcg acggctcgaa cacgacctcc caacccactt ccgccgtccg
      721 cggcgaggtg aactcgaccg cggcccagcc gtcgtggttg gaaatcttca cgggcaagag
      781 gccgcttgcg ccgcgcgccg tcgccttctt caccacgaag ttcttcccgc gcgggaacga
      841 gaagcgcagc tcgtacgggt cgttttttat aaccttgctg cgccccgcgt aagagccgcg
      901 cgcggcgtcg tagcggtgcg cgacgagatc gacccaccct tgcgtgatgt ggcggctcgt
      961 cgagatgagc tgcacgctgc cggacgcggg catcagcgtc agcacgcggc tgctcgcggg
     1021 cgcgaggtca atcgtcagcc cttgctccca ctcgccgagg tactccttgt tccagaagtc
     1081 gaagacctgc acgcgcgcgc cttccggcag gcccaagtcc ttccagccga cgtagagcgg
     1141 cgcctgtccg cgctcgtcga agttgaagag gccgaccacg tcgtaacggc gcccgaggtg
     1201 gttgaccttc aaatcccaga cgcgcttgtt gcgctcggac ttgaagaggt cgagcgggcg
     1261 cacgtccgcc gccggcatga cgcgccgcag cagctcgacg cgctccggcg acaagtccat
     1321 cagacggtcg ctcgtcagga cggcctgccc cgtcaagcct tgcagcgtcg cccacgcgcg
     1381 ggcctgctcc agggggagcg gcgcgcgcac gaccatcacg tccgggtctg cgtaccacgc
     1441 gacgttgtgc aggaagtaat actccatcgt cgcgcgcagc gcgaacttga aaccgtccca
     1501 gttcggcagg atgtcggcgc cggtgcgcga gccgtccatc agcccgacgc cttcgagcgg
     1561 gatgacccag cagcccagca gatagcgtcc ggggccgatg ccggcgcgga tgctttcgag
     1621 cgtgtcgcgg tagagcttgt ccgcgtccgt cggcgggttc cgcatcgctg ccttccggtt
     1681 gcggtactcg cggatgacga tgggctggcc gtcgatcttg aaatactcgt agccccagcc
     1741 cgacagcgtg gtgaacaagt ctttcagata cctctgccct tccggcgtcg agggatcgac
     1801 gaggaacctg ccttcccacg tgctcacgac cgaggtgccg tcgggcttga gcatgaaggc
     1861 gccggggttg ctccgcacca gcgcctcgtt gctctgcccg tgcggcgcga gccagattcc
     1921 gggcgtcagg ccgagcgact tgatgtaggc ggcgaggtgc ggcatgccgc cggggaaacg
     1981 tttgtcaatc gtcgtccagt cgcggttctc gccctgcccg cggcccacgc cctgccaccc
     2041 gtcgtcgatc tgcaccaggc cggcgccgta gtccttcagg tgctccgcga tccagcgcgc
     2101 gttgcgcttc acctcgtcct cgccgatctc ctgataataa aaataccacg agcaccaacc
     2161 ggcgggcggc agcgcgaacc gcgact
//
LOCUS       DBNGOH010000275         3441 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2056367, whole genome shotgun sequence.
ACCESSION   DBNGOH010000275 DBNGOH010000000
VERSION     DBNGOH010000275.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3441)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3441)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3441
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2056367"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(203..2302)
                     /locus_tag="AC159P_05210"
     CDS             complement(203..2302)
                     /locus_tag="AC159P_05210"
                     /inference="COORDINATES: protein motif:HMM:TIGR04312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor B family protein"
                     /protein_id="HIR3694884.1"
                     /translation="MNKRVVIGVAAALVAVAMLAWSATSSAHEGEHHSPGGNGQPDGA
                     ALNAMGPTPCTGGMAFTFPCNNIDLAALLPIADIDGTAEAGVTSNEVWGWTDPQTGKE
                     YAILGLSNGISFVDVSNPEAPVHLGNLPTHNVNSLWRSLKVYRNHVFVISEAANHGMQ
                     VFDLRQLRNTPAPPQVFTETAHYDRFASAHTININEETGFAYVAGSRPNPAGTANVNT
                     CGAARGLHVVDVKNPAAPAFAGCVDSDGYTHEAQCVVYRGPDAQYRDHEICFNANEDT
                     VTIVDVTNKAAPVQLSRTTYVGRGYTHQGWLTEDHRMLLVNDELDENGQQNPQTKTFV
                     WDVSDLDAPFVKNVQLLGTTAIDHNLYVRGRYVFESNYRSGLRVLDAANATTGALNQV
                     AFFDVFPADDQPLFNGSWHNYPYFASGTVLAAGIEQGLFVLRPRIDLPVKIDYAPFFV
                     RQHYLDFLSREPDEAGLGFWSHAIAACVADAPCTEVSRVNVSTAFFVSIEFLNTGFLV
                     HRFHAASFPNSLPTFQPFTADLAEIGEGIVVGRAGWEQELEANKRAFALDWVQRPAFV
                     AQFPANLPATDYVNALFTNAGANATQGERDAAVAAFGAGGTEGRALALRSVADSGSVY
                     NRQYNPAFVLMQYFGYLRRDPDQAGFQFWLDKLDSFTQPGEDVRDEQTALNRIRRAEM
                     VRAFITSIEYRARFGQN"
     gene            2510..3013
                     /locus_tag="AC159P_05215"
     CDS             2510..3013
                     /locus_tag="AC159P_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2199 domain-containing protein"
                     /protein_id="HIR3694885.1"
                     /translation="MSYRCAVCGEGHEDLPDIGMDKPDHWWDVPEEGRGERVELTSDT
                     CVIDGEDFFIRGVIELPIHDYPHAFGFGVWVSQKRENFVKYLENFDSAEIGPFFGWLC
                     TRIAFYEEDTQLLKTMAHFRGEGLRPRIELEPTDHPLAVAQREGITLDRAWEIVHFYK
                     NPNAPDA"
     gene            3041..>3441
                     /locus_tag="AC159P_05220"
     CDS             3041..>3441
                     /locus_tag="AC159P_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006422871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007030 - Golgi organization [Evidence
                     IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NRDE family protein"
                     /protein_id="HIR3694886.1"
                     /translation="MCLILCAYEVHPSYRLILAANRDEFYERPTQPAAFWPDAPGVLA
                     GRDLLHGGTWLGVTRAGRFAAVTNYRDPSAKLDGAPSRGALVADFLAGAESPAAYARD
                     ADARAAAYNGFNLLVGDARGLYYLSNRGGPPR"
BASE COUNT          547 a         1083 c         1236 g          575 t
ORIGIN      
        1 cagcgacgcc tgaatagtca acgcgcaatt cacgcgtcgc tgacgcgacg gcggcggatg
       61 gccgacgcca accccggcct tgaaaggccg ggctacgttc gatcgccgct ccgcggcgga
      121 taaggaagag gcttgagatt caacgtctca agcctctcgc ttcttccgtc ttaactgcct
      181 gctgcccact gcccgctact tctcagttct ggccgaagcg ggcgcggtat tcgatggagg
      241 tgatgaaggc tctgaccatc tcggcgcggc ggatgcggtt aagcgcggtc tgctcgtcgc
      301 gcacgtcctc gccgggctgc gtgaaggagt cgagcttgtc gagccagaac tggaagcccg
      361 cctggtcggg gtcgcggcgc aggtagccga agtattgcat gagcacgaag gccgggttgt
      421 actggcggtt gtagacgctg ccgctgtcgg cgacgctgcg cagcgcgagc gcgcgccctt
      481 cggtgccgcc cgcgccgaag gcggcgacgg cggcgtcgcg ctctccttgc gtcgcgttgg
      541 cgcccgcgtt cgtgaagagc gcgttgacgt agtcggtggc cggcaagttc gcggggaact
      601 gcgcgacgaa cgcggggcgc tgcacccagt cgagggcgaa cgcccgcttg ttggcttcaa
      661 gctcctgctc ccacccggcg cgcccgacga cgatgccttc gccgatctca gcgaggtcgg
      721 cggtgaaggg ctggaacgtg ggcaggctgt tggggaacga ggccgcgtgg aagcggtgga
      781 cgagaaagcc cgtgttcaga aactcgatgg agacgaagaa ggcggtcgag acgttgacgc
      841 gcgaaacctc cgtgcagggc gcgtcggcga cgcaagcggc gatggcgtgc gaccagaagc
      901 cgaggcccgc ctcgtccggc tcgcggctga ggaagtcgag gtagtgctgg cggacgaaga
      961 agggcgcgta gtcgatcttc accggcaggt cgatgcgcgg gcgcaggacg aacaggccct
     1021 gctcgatgcc tgcggcgagg acggtgccgc tggcgaagta ggggtagttg tgccacgagc
     1081 cgttgaagag cggctggtcg tcggcgggga acacgtcgaa gaaggcgacc tggttgagcg
     1141 cccccgtggt ggcgttggcg gcgtcgagca cgcgcaggcc gctgcggtag ttcgactcga
     1201 agacatagcg accgcgcacg taaaggttgt ggtcgatggc ggtcgtgccg agcagttgga
     1261 cgttcttgac gaagggcgcg tcgaggtccg acacatccca cacgaaggtc ttggtctgcg
     1321 ggttctgctg cccgttctcg tccagctcgt cgttgacgag cagcatccgg tggtcttccg
     1381 tgagccagcc ctggtgcgtg tagccgcgcc ccacgtaggt cgtgcgcgag agctggacgg
     1441 gggcggcctt gttcgtcacg tccacgatgg tgacggtgtc ctcgttggcg ttgaagcaga
     1501 tttcgtggtc gcggtactgc gcgtcggggc cgcggtagac gacgcactgc gcctcgtgcg
     1561 tgtagccgtc cgagtccacg cagccggcga aggcaggcgc cgcagggttc ttcacgtcca
     1621 cgacgtggag gccgcgcgcg gcgccgcacg tgttgacgtt ggccgtcccc gcggggttgg
     1681 ggcggctgcc ggcgacgtag gcgaagccgg tctcctcgtt gatattgatg gtgtgcgcgc
     1741 tggcgaagcg gtcgtagtgc gcggtctctg tgaagacctg cgggggcgcg ggcgtgttgc
     1801 gcagttgccg gaggtcgaag acctgcatgc cgtggttggc ggcctcgctg atgacgaaga
     1861 cgtggttgcg gtaaaccttc aggctgcgcc acaaagagtt gacgttgtgc gtcggcaggt
     1921 tgccgaggtg gacgggcgct tccgggttcg acacgtccac gaacgagatg ccgttcgaca
     1981 gaccgaggat ggcgtactcc ttgcccgtct gcgggtcggt ccagccccac acctcgttcg
     2041 aggtgacgcc cgcctccgcc gtgccgtcga tgtccgcgat gggcagcagc gccgccaggt
     2101 cgatgttgtt gcacgggaag gtgaaggcca tgccgcccgt gcagggcgtc ggccccatcg
     2161 cgttgagcgc ggcgccgtcc ggctggccgt tgccgccggg gctgtggtgc tcgccctcgt
     2221 gcgccgagga cgtggcggac cacgcgagca ttgcgacggc cacgagcgcg gcggccacgc
     2281 cgatcacaac tcttttattc atctttaggg ttccggttgg cgttacgact cgaaaggaca
     2341 acgaaggaca actccttcag acgccgtagg ccggcgggga gttgcacccg cggcggggca
     2401 aaaaccggcg gacggaagac agggcggctg attttacaag ccggggcggc gggaaggcaa
     2461 aaggcgggtg gctctgccgc gacgccgcaa tgcgtgtaga ataaaagcca tgagctaccg
     2521 ttgtgccgtc tgcggagagg gccacgaaga cctgccggac atcgggatgg acaagccaga
     2581 ccattggtgg gacgtgcccg aagaaggccg gggcgagagg gtcgaactca cgtcggacac
     2641 ctgcgtcatc gacggcgagg acttcttcat ccgcggggtc atcgagctac cgatacacga
     2701 ttacccgcac gccttcgggt tcggcgtctg ggtctcgcag aaacgcgaaa acttcgtcaa
     2761 gtatctggag aacttcgact ccgccgagat cggccccttc ttcggctggc tctgcacgcg
     2821 catcgcgttc tacgaagagg acacgcagtt gctgaagacg atggcgcact tccgcggcga
     2881 gggcctgcgc ccgcgcatcg aactggagcc gaccgaccac cccctcgccg tcgcacagcg
     2941 cgaaggcatc acgctggaca gggcgtggga gatcgtccac ttctacaaga atccgaacgc
     3001 gcccgacgcc tgacggcgcg cgcggcccga ccctccgtgt atgtgtctca tcctctgcgc
     3061 ctacgaagtt cacccgtcgt accgtctgat cctcgccgcc aaccgcgacg agttttacga
     3121 gcgcccgaca cagccggccg cgttctggcc ggacgcgccc ggcgtgctcg cgggccgcga
     3181 cctgctgcac ggcgggacgt ggctcggcgt cacgcgcgcg ggccgcttcg ccgccgtcac
     3241 caactaccgc gacccttcgg cgaagctcga cggcgcgccc tcgcgcgggg cgctcgtcgc
     3301 ggactttctc gcgggcgcag agtcgcccgc cgcttacgcg cgcgacgcgg acgcgcgcgc
     3361 cgccgcctat aacggcttca acctgctcgt cggcgacgcg cgaggtcttt actatttatc
     3421 gaaccgcggc ggcccgccgc g
//
LOCUS       DBNGOH010000276         4304 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2056685, whole genome shotgun sequence.
ACCESSION   DBNGOH010000276 DBNGOH010000000
VERSION     DBNGOH010000276.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4304)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4304)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4304
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2056685"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..777)
                     /locus_tag="AC159P_05225"
     CDS             complement(<1..777)
                     /locus_tag="AC159P_05225"
                     /inference="COORDINATES: protein motif:HMM:NF013584.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HIR3694887.1"
                     /translation="MDEIIYASASEIARAIRDGEVSAEEVTAAYLARIEEMNPKLNAF
                     VQVGRDAARGRARELDAALGRGELAGPLHGVPVSIKDAFDLAGVVTAGGTKGRAGFVP
                     QEDAAGVARLKAAGAVVLGKTNVPEMSLAFESDNLVYGRTKNPYDLARTPGGSSGGEA
                     AAIAAGLSPLGLGSDAGGSIRLPAHFCGIAGLKPTTGRTARTGHFPPPGGLFDSLWQI
                     GPLARRVEDLALALPLLCGADWRDPSVAPVPLEDPAAVEVE"
     gene            complement(910..2142)
                     /locus_tag="AC159P_05230"
     CDS             complement(910..2142)
                     /locus_tag="AC159P_05230"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012988713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HIR3694888.1"
                     /translation="MVRKKKLFISLFVIVALGAAAFFFVAPAAVESYYNRTLHAPPYA
                     ASERARALHAKLFVADLHADSLLWDRDILARSSRGHVDVPRLVEGGVSLQAFTIVTKV
                     PRGANYGVTEGDTDNITPLVVAGRWPARTWGSLTERALHQARKLEDAAARSGGRLVII
                     ESAGDLARFVEQRARASEDGRAVEASQSVGTSQSNGGARVAAWLGVEGAHALDGEVAN
                     LDRLFDAGVRMMAPVHFFDNEWGGSAHGARKGGLTEKGRELVRRMEAKGVVVDLAHAS
                     AQTIDDVLALATRPVVVSHTGVRGGCDNERNIDDERVRRIAATGGVVGIGFWEAATCG
                     TDAGSIARAIRYAANLVGARHVALGSDFDGAVATPFDATGLAQLTDALLAEGFDEAEI
                     ELIMGGNVLRVLAETLPR"
     gene            complement(2299..3399)
                     /locus_tag="AC159P_05235"
     CDS             complement(2299..3399)
                     /locus_tag="AC159P_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013377731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR3694889.1"
                     /translation="MAKEIGIGIIGTGFARTTQIPAFRACEGARVVSIASGRRENAER
                     VAREFDIAHAAGDWREVVARDDVHLVSIVTPPSTHAEMTLAALAAGKAVLCEKPMAMD
                     AGETDAMRRAARDRGLLAHIDHELRFLPARRKMREMISAGRVGSVRQLKFLFRSDMRA
                     SAARGWNWWSDREAGGGTLGAIGSHAVDTLSWLTGARVSHVSALLATHVAERADEESG
                     AARAVTSDDEADMLLHFAGGASEGHATGVVAMSVVEAGRPEHRVQVFGSEGALMLESG
                     GELFHAQVGEGAWRKIETASAPLAAGMRDNEWSRGFTVFAREIVAALREGRRAVEGAA
                     TFDDGHRIQLVLDAARAAHDGGCRVNVTGDEA"
     gene            complement(3546..>4304)
                     /locus_tag="AC159P_05240"
     CDS             complement(3546..>4304)
                     /locus_tag="AC159P_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006098539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HIR3694890.1"
                     /translation="AGRRSADLTDAKFMRLPGTAGEAQALGVLMPGATMLTGARATEA
                     ALKHVNGPRVLHVATHGFFLADQSRNAGEDARGIKLKKENAAVPTGENLLLRSGLALA
                     GANARRSDGGEDGILTALEAAGLDLWGTKLVVLSACETGLGDVKNGDGVYGLRRALVL
                     AGSESQVMSLWQVSDEATRDLMVAYYKRLQAGEGRTEALRQVQLEMIKGRSAAARDHT
                     RGLSGASMGAAATDRSHPFYWASFIQSGEWQPMK"
BASE COUNT          596 a         1595 c         1433 g          680 t
ORIGIN      
        1 ttcgacttcg accgccgcgg ggtcttcgag cggcacgggc gccaccgacg ggtcgcgcca
       61 gtccgcgccg cacagcagcg ggagcgcgag cgccaaatcc tcgacgcgcc gcgcgagcgg
      121 gccgatctgc cataaagaat cgaagagacc gccgggcggc gggaagtggc ccgtgcgcgc
      181 ggtgcgcccg gtggtcggct ttagccctgc gatgccgcag aagtgcgcgg gcaggcggat
      241 gctgccgccc gcgtcgctcc ccagtccgag cggcgacagc ccggcggcga tggccgccgc
      301 ctcgccgccg ctgctcccgc cgggcgtgcg cgcgaggtcg taaggattct tggtccgacc
      361 gtagacgagg ttgtcgctct cgaacgcgag cgacatctcc ggcacgttcg tcttgccgag
      421 cacgaccgcg cccgccgcct tcagccgcgc cacgcccgcc gcgtcctcct gcggcacgaa
      481 ccccgcgcgc cccttcgtcc cgcccgccgt cacgacgccc gccaagtcga acgcgtcctt
      541 gatcgacacc ggcacgccgt gcagaggccc cgccagctcg ccgcgcccga gcgccgcgtc
      601 caattcccgc gcccgcccgc gcgccgcgtc ccgtccgacc tgcacgaacg cgttcagctt
      661 cgggttcatc tcctcgatgc gcgcgaggta cgccgccgtc acctcctccg cggagacttc
      721 cccgtcccgg atggcccgcg caatctcgct cgcggacgcg tagatgatct cgtccatgat
      781 tcccctccga gctgttagcc gttagcgatt agctgttagc tttcggagtg acgaagcacc
      841 cggcgcgagc tgtcaacact cgattcgttc ctgaaattgc taacagctaa tcgctaacag
      901 ctaacagctt caccgcggca gcgtttccgc gagcacgcgg agcacgttgc cgcccatgat
      961 cagttcgatc tccgcttcgt cgaagccttc ggcgaggagc gcgtcggtga gctgcgcgag
     1021 gccggtcgcg tcgaagggcg tggcgacggc gccgtcgaag tccgagccga gcgcgacgtg
     1081 gcgcgcgccg acgaggttgg cggcgtaacg tatcgcgcgg gcgatcgacc ccgcgtccgt
     1141 gccgcacgtc gccgcctccc agaagccgat gccgacgacg ccgcccgtcg ccgcgatgcg
     1201 ccgcacgcgc tcgtcgtcga tgttgcgctc gttgtcgcaa ccgcccctga cgcccgtgtg
     1261 cgagacgacg accgggcgcg tcgccagcgc gagcacgtcg tcaatcgtct gcgcggaagc
     1321 gtgcgcgaga tcgaccacga cgcccttcgc ctccatgcgg cggacgagtt cgcgcccctt
     1381 ctccgtcagc ccccctttgc gcgcgccatg cgccgagccg ccccactcgt tatcgaagaa
     1441 gtgcacgggc gccatcatcc gcacgcccgc gtcgaacagc cggtcgaggt tcgccacttc
     1501 gccgtcgagc gcgtgcgcgc cctcgacgcc gagccacgcc gcgacccgcg cgccgccatt
     1561 cgattgactc gtaccgaccg actgactcgc ttcaacggct ctcccgtctt cgctcgcacg
     1621 cgcccgctgc tcgacgaagc gcgcgaggtc gcccgccgat tcgatgatga cgagccggcc
     1681 ccccgagcgc gccgccgcgt cttcgagctt gcgcgcctga tggagagcgc gctccgtcag
     1741 actcccccac gtccgcgcgg gccagcgccc cgcgacgacg agcggcgtga tgttgtccgt
     1801 gtcgccctcg gtcactccgt agttcgcccc gcgcggcacc ttcgtcacga tagtgaacgc
     1861 ctgaagcgag acgccgcctt cgacgaggcg cggcacgtcc acgtggccgc gcgaagaccg
     1921 cgcgaggatg tccctgtccc acagcagcga gtcggcgtgc aggtcggcga cgaacaactt
     1981 cgcgtggaga gcacgggcgc gctcggacgc cgcgtacggc ggcgcgtgca gcgtccggtt
     2041 gtaataagac tcgaccgccg cgggcgcgac gaagaagaac gccgcggcgc cgagcgcgac
     2101 gatgacgaag agggagatga aaagtttttt ctttctcacc agagcatgtt caggttggcc
     2161 gtggtacgtg cgcgacgatt gtaggggcag ggcttgtccc tgcccgccgt cacgcgataa
     2221 tgcgacgttg aatcgatcgt aggtcgcggg gcgcggactt accctgcccc tacggtattg
     2281 ccacggtctc tcgactcttc acgcttcgtc gcccgtcacg ttgacgcggc agccgccgtc
     2341 gtgcgcggcg cgggcggcgt cgaggacgag ctggatgcgg tggccgtcgt cgaaggtggc
     2401 ggcgccttcg accgcgcggc ggccttcgcg gagcgccgcg acgatctcgc gcgcgaagac
     2461 ggtgaagccg cgcgaccact cgttgtcgcg catcccggcg gcgagcggcg cgctcgcggt
     2521 ctcgattttg cgccacgcgc cttcgccgac ctgcgcgtgg aaaagctcgc cgccgctttc
     2581 gagcatgagc gcgccctcgg agccgaagac ctgcacgcgg tgttcgggcc ttcccgcttc
     2641 gaccaccgac atggcgacga cgccggtcgc gtgcccttcg cttgcgccgc ccgcgaagtg
     2701 gaggagcatg tccgcctcgt cgtcggaggt gacggcgcgc gcggccccgc tctcttcgtc
     2761 ggcgcgctcg gcgacgtgcg tcgccaggag cgccgagacg tgcgagacgc gcgcgcccgt
     2821 gagccacgag agcgtgtcca cggcgtgcga gccgatggcg ccgagcgtgc ccccgcccgc
     2881 ctccctgtcc gaccaccagt tccagccgcg cgcggcggag gcccgcatgt ccgagcggaa
     2941 caggaacttc agttggcgca cgctgccgac gcgccccgcg gagatcatct cgcgcatctt
     3001 ccgccgcgcg ggcaggaagc gcagctcgtg gtcgatgtgc gcgagcaggc cccggtcgcg
     3061 cgcggcccgt cgcatcgcgt cggtctcgcc cgcgtccatc gccatcggct tttcgcagag
     3121 cacggccttg cctgcggcga gcgccgcgag cgtcatctcc gcgtgcgtcg agggcggcgt
     3181 gacgatgctt accaggtgaa cgtcgtcgcg cgcgaccacc tcgcgccagt ccccggcggc
     3241 gtgcgcgatg tcgaactcgc gcgccacgcg ctccgcgttc tcgcgccgcc cgctcgcaat
     3301 cgacacgacg cgcgcgccct cgcacgcgcg gaaggcgggt atctgcgtcg tgcgcgcgaa
     3361 gcccgtgccg atgatgccga tgccgatctc tttggccatg acgttttctc gcccttcctt
     3421 actaaaagac tgagggctga ggactgagga ctgagtaaag cgttaatgat tcacgagagg
     3481 cgtgctgtac ggactcgtcc ggctcacgtc ttccactcag tcctcagtcc tcagtcctca
     3541 gtccttcatt tcatcggctg ccactcgccg gactggatga agctcgccca gtagaagggg
     3601 tggctgcggt ctgtggcggc ggcgcccatg ctcgcgcccg agaggccgcg ggtgtggtcg
     3661 cgcgccgccg ccgaccggcc ctttatcatt tcgagctgca cctgccggag cgcttcggtg
     3721 cgcccttcgc ccgcttgcag gcgcttgtag taggcgacca tcaagtcgcg tgtggcctcg
     3781 tccgagacct gccacaaact catcacctga ctctcggaac ccgcgagcac cagcgcccgc
     3841 ctcagcccgt acacgccatc gccgttcttc acgtctccca gtccggtctc gcaggccgag
     3901 agcacgacca gcttcgtgcc ccagaggtcg agtccggcgg cttcgagggc ggtgaggatg
     3961 ccgtcctcgc cgccgtcgct gcggcgcgcg ttcgccccgg cgagcgcgag gcccgagcgc
     4021 aacagcaggt tctcgccggt cggcaccgcc gcgttttctt ttttcaactt gatgccgcgc
     4081 gcgtcctcac cggcgttgcg cgactggtct gcgaggaaga agccgtgcgt ggcgacgtgc
     4141 agcacgcgcg ggccgttgac gtgtttgagc gcggcctccg tggcgcgcgc gccggtgagc
     4201 atggtcgcgc cgggcatgag cacgccgagc gcctgcgcct cgcccgccgt gccgggaaga
     4261 cgcatgaact tcgcgtcagt gaggtctgcc gagcggcgac ccgc
//
LOCUS       DBNGOH010000277         8327 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2606351, whole genome shotgun sequence.
ACCESSION   DBNGOH010000277 DBNGOH010000000
VERSION     DBNGOH010000277.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8327)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8327)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8327
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2606351"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..517)
                     /locus_tag="AC159P_05245"
     CDS             complement(<1..517)
                     /locus_tag="AC159P_05245"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: protein motif:HMM:NF001453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HIR3694891.1"
                     /translation="MSSAHTSGFRIPRERMVERLRDHYRIADPRVLAAMSEVPRHLFV
                     PEALAGRAYGDHALPIAANQTISQPYIVARMSELLEVNKESRVLEIGAGSGYQTAVLA
                     RLAGQVYAIERIADLAREAQARIRALGIYNATVKCFDGTLGWSAHAPYDGILVAAGGP
                     EVPGPLLAQLKV"
     gene            complement(654..1445)
                     /gene="surE"
                     /locus_tag="AC159P_05250"
     CDS             complement(654..1445)
                     /gene="surE"
                     /locus_tag="AC159P_05250"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015350480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HIR3694892.1"
                     /translation="MFRILLTNDDGIHSDGLIKLEEALREVGDVYTVAPASEMSGASH
                     SLTLSRPLRIRQIDERHWTVDGTPTDCVTLALHRILGEEERPHLCVSGINHGGNLGDD
                     ASYSGTVAGALEATILGVPGLAFSLVARENFDFTEAARFAVVTVRKVLETGLPEGTLL
                     NINIPRGQIRGARVTRQGIKHARPIISEHIDPRGKAYYWIGEEYYTSETEEGTDYHAV
                     ERGYASVTPMRSDMTDHNSLVALDAWNTITAAEVEREGEDQPAGR"
     gene            complement(1532..1756)
                     /locus_tag="AC159P_05255"
     CDS             complement(1532..1756)
                     /locus_tag="AC159P_05255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694893.1"
                     /translation="MSQDKKHEVKVKVQVLELSKAGSGIELEVFAEGEKLGVIEIGHG
                     SFGWRKASGKLGFKRVDWTTFTEWLNKKLY"
     gene            complement(1901..3937)
                     /locus_tag="AC159P_05260"
     CDS             complement(1901..3937)
                     /locus_tag="AC159P_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HIR3694894.1"
                     /translation="MRPIKHVEKGLSQVAGGLFNTIQFFNKYRPNESFTPKWSEKPLL
                     KSWQKSKPPLGFPRTTDSLCPKCVIEARTKILSAEVIDPSVLVNEKVGEIKATIVERN
                     GEIWMEKDCPIHGHFEDIMAIDSAFLKHIEKMFPGRDIQSHNDEKLHNHGSSTIKYGR
                     GAVLTIDLTNRCNMMCDPCFMDANQVGFVHELSMEEIQDILDKAISIKPRRQMSVQFS
                     GGEPTISPHFLESIRYSRKVGYNSVQCATNGIEFAKSKEFCREAAEAGLRYAYLQFDG
                     IGNDANSHRQVGNLFDVKLRAIDNLHEAGVEIVLVITLVNNINNDQVGSVIRFALDNP
                     KKIAFLSFQPVSFTGRDEEITDERRMAQRYTLSHLAQDVKSQVGITEPTRDWFPLSLM
                     GAFADFADLAHGPDAEWGQVSCGCHPNCGVGTAVMIDKETKDMAPVPAFLNIPGLVTD
                     MQKITDAARGKWTSNLLMGLALLKHYNSYNAPKAFSLYELFKKFDKSFGLTGRTYGRV
                     DGKRTKEDIEARRSDRWNFLFIAGMWFQDLFNYDFRRTEMCIIPYGTQEGEISFCAYN
                     TGIGWRNIIEQMHQNATVAKWYKEHGRHEIFAKGKEVPMAETPVSLVLNEVDLQRPHR
                     PEMAGPKTAAEEAAMMRKLYQEMVLKKKLGADVEKNTPVQIKSLSRSKTETTNA"
     gene            complement(4227..5012)
                     /locus_tag="AC159P_05265"
     CDS             complement(4227..5012)
                     /locus_tag="AC159P_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HIR3694895.1"
                     /translation="MSERELRELLDFGVGLARGAGEITLRYFRKKFDTRRKANKTFVT
                     EADLEAEAFLRREIAARYPEDAILGEEEGAREGTSGRRWIVDPIDGTYSFVHGVPFYG
                     VLVGLEIEGEPSVGVIHMPALGETAYAARGLGCFLNGETARVRETRTLEESLVLATDF
                     GTCGRYGFGRAADELQRRAGQRRTWGDCYGYLLVATGRADVMLDPVMNVWDCAALLPV
                     VEEAGGTFTDWQGQRTIHGGNAIATNGLLFEEVMAIVRSANAE"
     gene            complement(5009..5677)
                     /gene="hisIE"
                     /locus_tag="AC159P_05270"
     CDS             complement(5009..5677)
                     /gene="hisIE"
                     /locus_tag="AC159P_05270"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010500402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HIR3694896.1"
                     /translation="MKTRAEEVRFDGRGLVPAIVQDAKTREVLTLAYMNAESLRRTLE
                     SGETWFWSRSRGELWHKGETSGHTQRVVGLRADCDLDALVALVEPTGPACHTGARTCF
                     HHEMEGAAHEGSEATGGATSPQLGETLERLFRVVESRRGEMPEGSYTAYLFEKGLDKI
                     LKKVGEEAAETIIAAKNGDNNSLAAEVSDLLYHLVVLLVERGLTLEEVGRELERRSAP
                     KGAE"
     gene            complement(5674..6438)
                     /gene="hisF"
                     /locus_tag="AC159P_05275"
     CDS             complement(5674..6438)
                     /gene="hisF"
                     /locus_tag="AC159P_05275"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015529316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HIR3694897.1"
                     /translation="MLAKRIIPCLDVDRGRVVKGIRFVSLVDAGDPVEQARRYDEEGA
                     DELVFLDITASADKRAIVADLVRRVADEVFIPFTVGGGLNTIEDVRAVLRAGADKISM
                     NTAAVVRPELISEGAEVFGSQCVVVAIDARRRAAAASAGWAVFTHGGRRAVGWDAVEW
                     AARAERLGAGEILLTSMDRDGSLDGYDVELTRAVADAVRIPVIASGGAGTLEHFYEAL
                     TTGGASAVLAASLFHFGQFKVAEVKDYLRARGVVVR"
     gene            complement(6478..7083)
                     /gene="hisH"
                     /locus_tag="AC159P_05280"
     CDS             complement(6478..7083)
                     /gene="hisH"
                     /locus_tag="AC159P_05280"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HIR3694898.1"
                     /translation="MAIIDYGVGNLRSVEKAFQAGGTEAVVSSDERVLRGAERLVLPG
                     VGAFRACMSALRERGFDELVRERVAQGAPLLGVCVGMQMLFEESEEFGTTPGLGFLRG
                     RVRRFGEARRVPQVGWNQVEWSKGHALAAGIESGEFFYFVHSYFCAAEDGTVVVGETE
                     YGDRYASVVASANVCGVQFHPEKSQSAGLRLLKNFAEADSV"
     gene            complement(7097..7705)
                     /gene="hisB"
                     /locus_tag="AC159P_05285"
     CDS             complement(7097..7705)
                     /gene="hisB"
                     /locus_tag="AC159P_05285"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HIR3694899.1"
                     /translation="MGTELQTEARAAEVARKTKETDVRVRLVLDGTGRAAVSTGVAFL
                     DHMLELFARHGLFDLEVSCEGDLEIDDHHSVEDIAICLGQALGQALGDKAGVARYGMS
                     LVPMDEALCRAVVDLSGRFYLVYEVETRRQTIGNFSVELAEHFWRSFAEAARCNLHID
                     CLRGRNTHHILEGTFKATARALRQAVERDPRVRGVLSTKGVL"
     gene            complement(7771..>8327)
                     /locus_tag="AC159P_05290"
     CDS             complement(7771..>8327)
                     /locus_tag="AC159P_05290"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HIR3694900.1"
                     /translation="ERLLERTRGLVVVDEAYFEFAGQTVVPLLERHPNLAVFRTFSKA
                     LALAALRVGYLLAAPELAREVSKAVLPYNLNAFSQTAAEVSVEMYEAEILPTIRRIVA
                     ERDRLFEELRRVDGLAPVRSCANFMAVRSAIEPRRVYEEMLRRDILVRDVSGYPMLKE
                     YFRVSVGKPEENDRLIVALREIFA"
BASE COUNT         1352 a         2812 c         2609 g         1554 t
ORIGIN      
        1 cgaccttgag ctgtgcgagc agggggccgg gcacttccgg cccgcccgcc gcgacgagga
       61 tgccgtcgta gggcgcgtgc gcgctccacc cgagcgtgcc gtcgaagcac ttcacggtcg
      121 cattgtagat acccaaagcg cggatgcgcg cctgcgcttc gcgcgcgagg tctgcgatgc
      181 gctcgatggc gtagacctga ccggcgaggc gcgcgagcac cgcggtctgg tagccggagc
      241 cggcgccgat ctcgagcacg cggctctctt tgttgacttc cagcaattcg ctcatgcgcg
      301 cgacgatgta aggctgcgag atggtctggt tggccgcgat gggcaaggcg tggtcgccgt
      361 aggcgcggcc cgcgagcgcc tcggggacga agaggtggcg cggcacctcg ctcatcgccg
      421 cgagcacgcg cgggtctgcg atgcggtagt ggtcgcgcag gcgctcgacc attcgctcgc
      481 gcgggatgcg gaagcccgac gtgtgcgctg atgacaagtt ggaagagttt gaagcggaag
      541 accccaagaa cttatacccg ttcgaaagag gctggaggct ggaggctaga gactagtttg
      601 aaagtgatgc tgttttaact agcctctagt ctccagcctc cagcctcact tactcacctg
      661 cccgcgggct gatcctcgcc ctcgcgctcg acctccgcgg cggtgatcgt gttccacgcg
      721 tcgagcgcga ccagagagtt gtggtcggtc atgtcgctgc gcatcggcgt gacggaggcg
      781 tagccgcgct cgacggcgtg gtagtccgtg ccctcctcgg tctcggaggt gtagtactct
      841 tcgccgatcc agtagtaagc cttgccgcgc gggtcgatgt gctcgctgat gatggggcgc
      901 gcgtgcttga tgccctgccg cgtgacacgc gcgccgcgaa tctggccgcg cgggatattg
      961 atgttgagca gagtgccttc gggcaggccc gtctccaaca ccttgcgcac ggtgacgacg
     1021 gcgaagcgcg ccgcctccgt gaagtcgaag ttctcgcgcg cgaccagact gaaggcgagg
     1081 cccggcacgc cgaggatggt cgcttcgagc gcgcccgcca cggtgcccga atagctcgcg
     1141 tcgtcgccga ggttgccgcc gtggttgatg cccgagacgc agaggtgcgg gcgctcctct
     1201 tcgccgagga tgcggtgcag cgcgagcgtc acgcagtcgg tcggcgtgcc gtccaccgtc
     1261 cagtggcgct cgtcgatctg ccggatgcgc agcgggcgcg agagcgtcag gctgtgcgaa
     1321 gcgccgctca tctccgaggc gggcgcgacc gtgtacacgt cgcccacttc cctgagcgct
     1381 tcttcgagtt tgatgaggcc gtcggagtgg atgccgtcgt cgtttgtcag aagaatgcga
     1441 aacataaatt accgaaatcc attacaaccc atgcgaccga tttattcaag cgggcgcatg
     1501 ttcagtgtgc ggaaactcgc gccccacacg ttcagtacag tttcttgttc aaccactcgg
     1561 tgaacgtcgt ccaatcgacg cgcttgaagc cgagcttgcc gctcgccttg cgccagccga
     1621 aagagccgtg gccgatctcg atgacgccca gcttctcgcc ctcggcgaag acctcaagct
     1681 cgatgccgct cccggccttc gacagttcga gcacctgaac tttgactttc acttcgtgct
     1741 tcttgtcctg cgacattgtg cgcctcccgt ccgtgcgagt cgtctttcta ccgtggaaac
     1801 aggcgacgtt atcttacccc tcagccgttg acggcaaaag ccccgcgggg cgcgtccttg
     1861 cgtcccgcgg ggcttcggcg gttgatgaaa cgctcgccgc ttaggcgttc gtcgtctctg
     1921 tcttgctgcg cgagaggctc ttgatctgca ccggcgtgtt cttctccacg tcggcgccga
     1981 gcttcttctt caagaccatc tcctggtaga gcttgcgcat catcgcggcc tcttcggcgg
     2041 cggtcttcgg cccggccatc tcggggcggt gcggacgctg caagtcaacc tcgttcagca
     2101 cgaggctgac cggcgtctcg gccatcggca cctctttgcc cttggcgaag atttcgtggc
     2161 gcccgtgctc cttgtaccac ttggcgacgg tcgcgttctg gtgcatctgc tcgatgatgt
     2221 tgcgccagcc gatgcccgtg ttgtaggcgc agaaactgat ctcgccctcc tgcgtgccgt
     2281 aggggatgat gcacatctcg gtgcggcgga agtcgtagtt gaacaggtcc tggaaccaca
     2341 tgcccgcgat gaagaggaag ttccagcggt cggagcggcg cgcctcgatg tcctctttgg
     2401 tgcgcttgcc gtccacgcga ccgtaggtgc ggcccgtcag gccgaacgac ttgtcgaact
     2461 tcttgaacag ctcgtaaagc gagaaggcct tcggcgcgtt gtaggagttg tagtgcttca
     2521 gcagcgcgag gcccatcaac aggttcgaag tccacttgcc gcgcgccgcg tccgtgatct
     2581 tctgcatgtc cgtgaccaga cccggaatgt tcaggaaggc cggcacgggc gccatgtcct
     2641 tcgtctcctt gtcgatcatg accgccgtgc cgacgccgca gttcgggtgg cagccgcacg
     2701 agacctgacc ccattcggcg tccggcccgt gcgcgaggtc ggcgaagtcc gcgaaggcgc
     2761 ccatcaacga gagggggaac cagtcgcgcg tcggctcggt gatgccgacc tggcttttca
     2821 cgtcctgcgc gaggtgcgag agggtgtagc gctgcgccat gcggcgctcg tcggtgatct
     2881 cctcgtcgcg gcccgtgaac gagacgggct ggaacgagag gaaggcgatc ttcttcgggt
     2941 tgtcgagcgc gaagcggatg accgagccga cctggtcgtt gttgatgttg ttgacgagcg
     3001 tgatgacgag cacgatctcg acgcccgctt cgtgcaggtt gtcgatggcg cgcagcttca
     3061 cgtcgaacag gttgccgacc tgccggtgcg agttggcgtc gttgccgatg ccgtcgaact
     3121 gcaagtaggc gtaacgcagc ccggcctcgg cggcctcgcg gcagaactct ttcgacttgg
     3181 cgaactcgat gccgttggtc gcgcactgga cggagttgta gccgaccttg cgcgagtagc
     3241 ggatggattc gaggaagtgc ggcgagatgg tcggctcgcc gcccgaaaac tgcaccgaca
     3301 tctggcggcg cggcttgatg gaaatcgcct tgtcgaggat gtcctgaatc tcctccatgc
     3361 tcagctcgtg gacgaagccg acctggttgg catccatgaa gcacgggtcg cacatcatgt
     3421 tgcagcggtt cgtgaggtcg atcgtcaaga ccgcgccgcg cccgtacttg atggtcgagg
     3481 agccgtggtt gtgcagcttc tcgtcgttgt gcgactggat gtcgcgaccg gggaacatct
     3541 tctcgatgtg cttgaggaag gccgagtcga tggccatgat gtcctcgaag tggccgtgga
     3601 tggggcagtc cttctccatc cagatttcgc cgttgcgctc gacgatggtc gccttgatct
     3661 cgccgacctt ctcgttgacg agcacgctgg ggtcgatcac ctcggcgctc aggattttcg
     3721 tgcgcgcctc gatgacgcac ttcggacaca ggctgtcggt cgtgcgcggg aagccgagcg
     3781 gcggcttcga cttctgccat gatttcagga gcggcttctc ggaccacttg ggcgtgaaag
     3841 actcgttcgg cctgtacttg ttgaaaaact gaatcgtatt gaacagtccg cccgcgactt
     3901 gggacagacc tttctcgacg tgtttgatcg gtcgcatttt ctttccttct ctgaagattg
     3961 aacttaatta agccgtgggg cgcagcccgc aaagcggtat gcgccctttc agtggcaatg
     4021 actgtgccaa ccgcccctga ttctccaagt gttgaaataa ttaggctaaa gcggaagaag
     4081 cggaagtttg gagagtaaaa ttgaacggct ttttcgggtc atttggcgtc gatttatcta
     4141 ctgaaggacg gcgtgttgcc ttctgacccg ccaagccttg attttgcgga cgtgtgtgca
     4201 cttattgact cgcggctctg tcaccgctat tcggcgttcg cggaacgcac gattgccata
     4261 acctcttcga aaagcaggcc gttagtcgct atcgcgttac cgccgtggat ggttctctgc
     4321 ccctgccagt ccgtgaaagt cccgcccgcc tcttcgacga cgggcaacaa cgccgcgcag
     4381 tcccacacgt tcatcacagg gtcgagcatc acgtctgccc gccccgtcgc gacgagcagg
     4441 tatccgtagc aatcgcccca cgtgcgcctt tgtcccgcgc gccgctgcaa ttcgtccgcg
     4501 gcgcgaccga agccgtagcg cccgcacgtg ccgaagtcgg tcgcaagcac cagagactct
     4561 tcgagcgtac gggtctcgcg gacgcgcgcc gtctccccgt tcaggaagca cccgaggccg
     4621 cgcgccgcgt aggccgtctc gccgagcgcg ggcatgtgta tcacgccgac cgacggctcg
     4681 ccttcgatct ccaggccgac gagcacgccg tagaacggca cgccgtggac gaacgagtac
     4741 gtcccgtcaa tcggatcgac gatccagcgc ctgccggaag tgccttcgcg cgcgccctcc
     4801 tcttcgccga gtatggcgtc ttcgggatag cgcgccgcga tctcgcgccg gaggaaggct
     4861 tcggcctcaa ggtcggcctc ggtcacgaac gtcttgttcg ccttgcggcg cgtgtcgaac
     4921 ttcttgcgga agtagcgcag cgtgatctcg cccgcgccgc gcgccagacc gacgccgaag
     4981 tcaagaagct ctcgcagttc tctctcgctc attcagcccc tttcggcgca ctcctgcgct
     5041 caagctcgcg gcccacttct tcgagcgtca acccgcgttc gactaataac acgacgagat
     5101 ggtagagcaa gtccgagacc tccgcggcca gcgagttgtt atcgccgttc ttcgccgcga
     5161 tgatcgtctc cgccgcctcc tcgccgacct tcttcagaat cttgtccagc cccttctcga
     5221 agaggtaggc cgtgtaagag ccttccggca tctcgccgcg ccgcgactca accacccgga
     5281 agagtcgttc cagcgtctct cccaactgcg gactcgtcgc cccgcccgtc gcctccgagc
     5341 cttcgtgcgc cgcgccctcc atctcgtgat gaaagcaggt gcgcgcgccg gtgtggcacg
     5401 ccggcccggt gggttcgacg agcgcgacga gcgcgtcgag gtcgcagtcg gcgcgcaggc
     5461 cgacgacgcg ctgcgtgtgg ccggacgttt cgcccttgtg ccacagctct ccgcgcgagc
     5521 gcgaccaaaa ccacgtctcg ccgctttcga gcgtgcgccg caagctctcc gcgttcatgt
     5581 acgcgagcgt caacacctcc cgcgtcttcg cgtcctgcac gattgccggg acgagtccgc
     5641 gcccgtcgaa acgcacctct tccgctctcg tcttcatctc accacgaccc cgcgcgcgcg
     5701 caggtagtct ttcacctccg cgaccttgaa ctgcccgaag tggaacagcg acgcggcgag
     5761 cacggcgctc gcgccgcccg tcgtcagcgc ctcgtaaaaa tgttcgagcg tgccggcgcc
     5821 gcccgaagcg atgaccggga tgcgcacggc gtcggcgacc gcgcgcgtca gctccacgtc
     5881 gtagccgtcg agcgagccgt ccctgtccat gctcgtcaga agtatttcgc ccgcgccgag
     5941 gcgttcggcg cgcgccgccc actcgaccgc gtcccacccg accgcgcgcc gcccgccgtg
     6001 cgtgaagacc gcccagcccg cggatgcggc ggcggccctg cgacgcgcgt cgatggcgac
     6061 gacgacgcac tggctgccga agacctccgc gccctcgcta atcaactcgg gccgcacgac
     6121 tgccgccgtg ttcatcgaaa tcttgtcggc gcccgcgcgc aacaccgcgc gtacgtcctc
     6181 aatcgtgttc agcccgccgc cgaccgtgaa cgggatgaag acctcgtccg ccactcgccg
     6241 cacgaggtcg gcaacgatgg cgcgcttgtc cgcagaggcc gtgatgtcga ggaagaccaa
     6301 ctcgtcggcg ccctcctcgt cgtaacgccg cgcctgctcc acagggtcgc ccgcgtccac
     6361 cagcgacacg aagcggatgc ccttgaccac acgcccgcgg tcaacgtcga ggcagggaat
     6421 gatgcgtttc gccagcatga tttgttggaa tcgagaagct gccaatcccc ttctcgtcta
     6481 aaccgaatcg gcctcagcga agttcttcag cagtcgcagc cccgcggact ggctcttctc
     6541 ggggtggaac tgcacgccgc acacgttcgc gctcgcgacg accgaggcgt acctgtcgcc
     6601 gtactccgtc tcgccgacga cgaccgtgcc gtcctccgct gcgcaaaagt aggagtgtac
     6661 gaagtagaag aactcgccgc tctcgatgcc cgccgcgagt gcgtgcccct tcgaccactc
     6721 gacctgattc cacccgacct gcgggacgcg ccgggcctct ccgaagcgac gcacgcgccc
     6781 gcgcaggaag ccgaggcccg gcgtcgtgcc gaactcctcc gactcttcga agagcatctg
     6841 catcccgacg cacacgccga gcagcggcgc accttgcgcg acccgctcgc gcaccagctc
     6901 gtcgaagccg cgctccctga gcgccgacat gcacgcgcgg aaggcgccga cgccgggcag
     6961 caccagcctc tccgcgccgc gcagcacgcg ctcgtccgag ctgacgaccg cctccgtccc
     7021 gcccgcctga aaagccttct ccaccgagcg caggttgccg acgccgtaat cgatgatcgc
     7081 cacgcgagac aattttctac agcacccctt tcgtgctgag cacgccgcgg acgcgcgggt
     7141 cgcgctcgac cgcctgccgc agcgcgcgcg ccgtcgcctt gaacgtgcct tcgaggatgt
     7201 ggtgcgtgtt gcgtccgcgc aggcagtcga tgtgcaggtt gcaccgcgcg gcctccgcga
     7261 acgagcgcca gaagtgttcg gccagctcga ccgagaagtt gccgatggtc tgccgccgcg
     7321 tctccacctc gtagacgaga tagaagcggc ccgacagatc aaccaccgcg cggcacaaag
     7381 cctcgtccat cggcaccaaa ctcatgccgt agcgcgcgac gccggccttg tcgccgagcg
     7441 cctgcccgag cgcctgcccg agacagatgg cgatgtcttc gaccgaatga tggtcgtcga
     7501 tctcaagatc gccctcgcac gacacttcga ggtcgaacag cccgtgccgc gcgaacagct
     7561 ccagcatgtg gtcgaggaac gcgacgcccg tagacaccgc cgcgcgcccc gtcccgtcga
     7621 gcacgagacg cacgcgcacg tccgtctcct tcgtcttgcg cgcgacctcc gccgcgcgcg
     7681 cctccgtttg cagttcggtg cccatacgtc ttcctcgctc cgaaatacga aagctcgact
     7741 ttcgcattcg agctttcaac tccgccccgc tcacgcgaag atttcgcgca gcgcgacgat
     7801 caggcggtcg ttctcttccg gcttgccgac gctgacgcgg aaatattcct tgagcatcgg
     7861 gtagccgctc acgtcgcgga cgaggatgtc gcggcgcagc atctcttcgt agacgcgccg
     7921 cggctcgatg gccgagcgca ccgccatgaa gttcgcgcac gagcgcacgg gcgcgaggcc
     7981 gtccacgcgc cgcagctctt cgaacaggcg gtctcgctcg gcgacgatgc ggcggatcgt
     8041 cgggaggatt tccgcctcgt acatctcgac ggagacttcg gcggcggtct gcgagaaggc
     8101 gttgaggttg tacggcagca ccgccttcga cacctcgcgc gcgagttcgg gcgcggccag
     8161 caggtagccg acgcgcagcg ccgccagcgc caacgccttc gagaaggtac ggaatacggc
     8221 gaggttcggg tggcgttcga gcagcggcac gaccgtttgc cccgcaaact cgaaataggc
     8281 ttcatctacg acgacgagac cgcgcgtccg ctccagcagc cgttcga
//
LOCUS       DBNGOH010000278         5228 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4989752, whole genome shotgun sequence.
ACCESSION   DBNGOH010000278 DBNGOH010000000
VERSION     DBNGOH010000278.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5228)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5228)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5228
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4989752"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..850
                     /locus_tag="AC159P_05295"
     CDS             <1..850
                     /locus_tag="AC159P_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HIR3694901.1"
                     /translation="TEGAAATGLRQGTPVVAGGGDQAAGAVGMGIVRAGAVSATVGTS
                     GVVFAATDRPALDPRGRVHTFCHAVPGRWHIMGVTQGAGLSLRWFRDQFGAGAEDGRD
                     PYERLSEEAAAAPPGSDGALWAPYLMGERTPHLDPHARAALVGLTASHTRGHVVRALL
                     EGVAFSLRDTLTIFAEMNVPVESIRLGGGGARSKVWRQIQADVYGREVETVEAEEGAA
                     YGAALLAGVGGGAWASVDAACDEVVRVASRTAPDAAAVALLDRQYEAFRALYPALRSI
                     TKAQSQ"
     gene            complement(1050..1997)
                     /locus_tag="AC159P_05300"
     CDS             complement(1050..1997)
                     /locus_tag="AC159P_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008834695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3694902.1"
                     /translation="MMRKELGTFILLVVLCVVVAIMNPVFLSATNLQNMARLIGMYGI
                     FSIGVGLVIITGGIDLSVGSVFALLGVLLSMALVEWGMPSGLAVLLVIAVATGLGLLH
                     GFLITKVRMQPFIVTLCGLLFYRGLARFVADDETKGFGAGGGFETLRNLATGSLFGVP
                     MPFVLLIVIAAVMWVVLHRSVYGRYLFAVGRNEVAARYSGVNSSLIVASAYVLCGLLA
                     GVAGVVLAFYTNSISPSSHGNAYELYGIAAAVLGGCSLRGGEGSIIGILIGAALLQVL
                     QNLVNLLGIPSSLNFAVMGVVILLGVMADQLSQRRSTAK"
     gene            complement(2086..3585)
                     /locus_tag="AC159P_05305"
     CDS             complement(2086..3585)
                     /locus_tag="AC159P_05305"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018069521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HIR3694903.1"
                     /translation="MSAPILEMKGIGKRFPGVVALESVDLEIYPGEVVALAGENGAGK
                     STLMKILGGVYQPDGGEIRIDGEEAAIHSVTDATRYGIGFVHQELNVLDNLNIGENVY
                     LGREPVWGGPLRLVDRDKIHADTQVHLRRLGLDIPSRTPLSRLSIAQQQMVEIAKALS
                     LDARILIMDEPTSSLTLTETERLLGVSKELRASGVSIIYISHRLGEIGELADRVVVLR
                     DGRNAGALRREEINHDNIVKLMVGRDIKHFYEPSAAVPQPGFVEVRELRTRRYPERAV
                     SFDIGKGEILGFAGLVGAGRSEVAQAVFGVDAPVGGTVTLDGTPVRISSPRDTIARGI
                     YLIPEDRRNSGLLLDFRIRENVTLPSLDRYASAGLIKYDAEQKVAEEMRGKLNIKAPT
                     VEARAGNLSGGNQQKVVLAKWLSLDPRLLIFDEPTRGIDVGAKAEIYHLMRQLAESGV
                     AIMMISSDMEEILGESDRVAVMHEGNITGILEREQCSEEAIMRLAVGKQ"
     gene            complement(3705..4724)
                     /locus_tag="AC159P_05310"
     CDS             complement(3705..4724)
                     /locus_tag="AC159P_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HIR3694904.1"
                     /translation="MKKISIALVCLCALAGLLALACAPAENTNSTTNAGGGTGGASSS
                     SGAQRKLAFVTNNASDFWTIARKGVEQADTELANVTVEFKIPSEGTAADQKRVVDDLL
                     AKGIDGMAISPVDPDNQTQLINDASKKVLVVTQDSDAPTSDRACYIGTDNVAAGRQAG
                     DLIKEALPQGGKIMVFVGKSDARNAAERFQGIKESLQGSNIQIIDLRTDDTDRVRAKS
                     NAADTLVKYPDVVALVGLWSYNGPAILNAVKDANKVGQVKIIAFDEEDETLAGVKDGA
                     IFATVVQQPYEFGYQSVKLMEKVLAGDKSAIPANKQIIVPTLVVKKDTVEEFTAKINK
                     LRGRG"
     gene            complement(4766..>5228)
                     /locus_tag="AC159P_05315"
     CDS             complement(4766..>5228)
                     /locus_tag="AC159P_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012255969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR3694905.1"
                     /translation="ENLGEQHDPETHLLPNVMAAAMGGLPYVSVFGADYPTKDGTAVR
                     DYIHVADLGEAHILALKHLREGGESERINLGNGQGYSVLEVIETARQVTGRPIEARME
                     PRRAGDPSHLVADAAKAHTLLGWRPRYPDLAAIIRTDWEWRQKHPRGYDPA"
BASE COUNT          808 a         1739 c         1779 g          902 t
ORIGIN      
        1 caccgagggc gcggcagcaa cagggcttcg ccagggcacg ccggtggtgg ccggcggcgg
       61 cgaccaggcc gcgggcgcgg tcggcatggg catcgtgcgc gcgggcgcgg tgagcgccac
      121 ggtcggcacg tccggcgtcg tgtttgccgc caccgatcgg cccgcgctcg atcccagggg
      181 acgcgtgcac acgttctgtc acgcggtccc gggccgctgg cacatcatgg gcgtgacgca
      241 gggcgcggga ctcagcctgc gctggttccg cgaccagttc ggcgcgggcg ccgaggacgg
      301 gcgcgaccct tacgaacgac tgtcggaaga ggcggcagcc gcgccgcccg gcagcgacgg
      361 cgcgctgtgg gcgccctacc tgatgggcga gcgcacgccg cacctcgacc cgcacgcacg
      421 cgccgcgctc gtcggcctga cggcgagcca cacgcgcggc cacgtcgtcc gcgccctgct
      481 cgaaggcgtc gccttttctt tgcgcgacac gctgacgatc ttcgcggaga tgaacgtgcc
      541 ggtcgagtcg atacgcctgg gcggcggggg cgcgcgctcg aaggtgtggc ggcagataca
      601 ggccgacgtg tacgggcgcg aggtcgaaac ggtcgaagcc gaagagggcg cggcctacgg
      661 cgcggcgctg ctcgccgggg tcggcggcgg cgcttgggcc tcggtggacg cggcctgcga
      721 cgaggtcgtc cgcgtcgcct cgcgcaccgc gcccgacgcc gcggcggtcg ccctgttgga
      781 tcgtcagtac gaagccttcc gcgccctcta cccggcgctg cgctccatca cgaaagcgca
      841 atcgcagtag gcagtaggca gtaggcagta ggcagtcaag acagaagagc gaagaagctt
      901 gagaggtaaa gtcccaagct cctttgctct tcaagcggta aagcgagagg cttgagacgt
      961 agcgtctcaa gcctcttctg acttccgact tccgacttcc gacttctgtc ttgactgcct
     1021 accgtttacc gcctactgcc tacttcttcc tacttggccg tcgagcggcg ctgcgagagc
     1081 tggtcggcca tgacgccgag gaggatgacg acgcccatca cggcgaagtt gagcgaggag
     1141 gggatgccga gcaggttgac gaggttctgc aagacctgca agagcgccgc gccgatgagg
     1201 atgccgatga tcgacccctc gccgccgcgc aaagagcagc cgcccaagac cgccgccgcg
     1261 atcccgtaca gctcgtacgc attgccgtgc gacgacggcg agatcgagtt cgtgtagaag
     1321 gccagcacca cgcccgccac gcccgccagc agcccgcaca gcacgtaggc gctcgctacg
     1381 atcaaagacg agttgacgcc cgagtagcgc gccgccacct cgttgcgccc caccgcgaag
     1441 aggtagcgcc cgtagaccga gcggtggagc accacccaca tcaccgccgc gatgacgatg
     1501 agcaggacga acggcatcgg gacgccgaag aggctgcccg tcgccaggtt gcgcagcgtc
     1561 tcgaagccgc cgccggcccc gaaccctttc gtctcgtcgt cggcgacgaa gcgcgcgagg
     1621 cccctgtaga agagcagccc gcagagcgtg acgatgaagg gctgcatccg caccttcgtg
     1681 atcaggaagc cgtggagcag ccccaatccg gtcgccaccg cgatcaccag cagcaccgcc
     1741 agcccactcg gcatccccca ctcgaccagc gccatcgaga gcagcacgcc cagcagcgcg
     1801 aagaccgacc cgaccgacag gtcgatcccg cccgtgatga tgaccagccc cacgccgatg
     1861 ctgaagatgc cgtacatccc gatcagccgc gccatgttct ggaggttggt cgccgacaga
     1921 aagaccgggt tcatgatggc caccaccacg cacagcacca cgagcaggat gaaggttccg
     1981 agttcttttc tcatcactca acctcgaaag ccgtgaaccg taagccgtaa accgtgataa
     2041 gtgaaacccc gtttctttct tgtcacggtt tacggttcac ggcttttatt gttttccgac
     2101 ggccagccgc atgatcgcct cctcgctgca ctgctcgcgt tcgaggatgc cggtgatgtt
     2161 gccctcgtgc atgacggcga cgcggtcgct ctcgccgagt atctcctcca tgtcggagga
     2221 gatcatcatg atggcgacgc cgctctcggc gagctggcgc atgaggtggt agatttcggc
     2281 cttcgcgccc acgtcgatgc cgcgcgtcgg ctcgtcgaag atgaggagcc gcgggtccag
     2341 cgagagccac ttcgcgagca cgaccttctg ctggttgccg cccgagaggt tgccggcgcg
     2401 cgcctcgacg gtgggcgcct tgatgttgag cttgccgcgc atctcctcgg cgaccttctg
     2461 ctcggcgtcg tacttgatga gacccgccga ggcgtagcgg tcgagcgagg ggagcgtgac
     2521 gttctcgcgg atgcgaaagt cgagcaggag gcccgagttg cggcggtctt ccgggatgag
     2581 gtagatgccg cgcgcgatgg tgtcgcgcgg cgaggagatt ctgaccggcg tgccgtcgag
     2641 cgtgacggtg ccgccgacgg gcgcgtccac gccgaagacg gcctgcgcca cctcggagcg
     2701 gcccgcgccg accagccctg cgaagccgag aatctcgccc ttgccgatgt cgaaagagac
     2761 cgcgcgctcc gggtagcggc gcgtgcgcag ctcgcggact tcgacgaagc cgggctgcgg
     2821 caccgccgcc gaaggctcgt agaagtgctt gatgtcgcgg cccaccatca gcttgacgat
     2881 gttgtcgtgg ttgatctcct cgcgcctgag cgcgcccgcg ttgcgcccgt cgcgcaggac
     2941 gacgacgcgg tcggccagct cgccgatctc gcccagacgg tgcgagatgt agatgatgct
     3001 cacgcccgag gcgcgcagct ccttcgagac cccgagcagc cgctcggtct cggtcagggt
     3061 gaggctcgaa gtcggctcgt ccatgatgag gatgcgcgcg tcgagcgaga gcgccttggc
     3121 gatctcgacc atctgctgct gcgcgatgga gaggcgcgag agcggcgtgc ggctcgggat
     3181 gtcgaggccg agccgccgga ggtggacttg cgtgtcggcg tgaatcttgt cgcggtcaac
     3241 cagacggagc ggcccgcccc acaccggctc ccggcccagg tagacgttct cgccgatgtt
     3301 caggttgtcg aggacgttca gctcctggtg gacgaagccg atgccgtagc gcgtcgcgtc
     3361 cgtgaccgag tggatggcgg cctcctcgcc gtcgatgcga atctcgccgc cgtccggctg
     3421 gtagacgccg ccgaggattt tcatcagcgt ggacttgccg gcgccgttct cgcccgccag
     3481 cgccacgacc tcgcccggat aaatctccag atcgaccgat tcgagcgcga ccacgccggg
     3541 gaaccgcttg ccgatcccct tcatttcgag aatgggagca ctcatagtgg tcagtggccg
     3601 gtggtcagtg gtcagtgaaa acagtggtgg gtggtcggtg gtcagtggtc agtgaaagac
     3661 aaacgcttct acggaccact gaccaccgac tactgaccac tgcgctagcc gcgtccgcgg
     3721 agcttgttga tcttggcggt gaactcctcc acggtgtctt tcttgacgac gagcgtgggg
     3781 acgatgatct gtttgttggc ggggatggcg gacttgtcgc cggccagcac cttctccatc
     3841 agcttgacgg actggtagcc gaactcgtat ggctgctgca cgacggtcgc gaagatggcg
     3901 ccgtccttga cgcccgcgag cgtctcgtcc tcttcgtcga aggcgatgat cttgacctgc
     3961 ccgaccttgt tcgcgtcctt cacggcgttg aggatggccg ggccgttgta gctccacagc
     4021 ccgacgagcg cgaccacgtc cgggtacttc accagcgtgt cggcggcgtt cgacttggcg
     4081 cgcacgcggt ctgtgtcgtc ggtgcgcagg tcgatgatct ggatgttcga gccttgcagc
     4141 gactccttga tgccctggaa gcgctcggcg gcgttgcgcg cgtcggactt gccgacgaag
     4201 accatgatct tgccgccctg cggcagcgcc tccttgatga ggtcgcccgc ctgccttccg
     4261 gcggcgacgt tgtcggtgcc gatgtagcac gcgcggtcgc tcgtgggcgc gtcgctgtcc
     4321 tgcgtcacga cgagcacctt cttggaggcg tcgttgatga gctgcgtctg gttgtcggga
     4381 tcgaccgggc tgatcgccat gccgtcgatg cccttggcca gcaggtcgtc caccacgcgc
     4441 ttctggtcag ccgccgtgcc ctcggaagga atcttgaact cgaccgtcac gttcgcgagt
     4501 tccgtgtcgg cctgctcgac gcccttgcgc gcgatggtcc agaagtccga cgcgttgttg
     4561 gtgacgaagg ccagcttgcg ctgcgccccg ctgctgctgc tcgcgccgcc cgtcccgccc
     4621 cctgcgttcg tcgtgctgtt ggtgttctcc gcaggggcgc aggccagggc cagcaggccc
     4681 gcgagcgcgc acaggcaaac gagcgcgata ctgattttct tcatgccggt ttcctctcgt
     4741 atcggatgtg gtctacggaa ccttttcacg ccgggtcgta accgcgcggg tgcttctgcc
     4801 gccactccca gtcggtgcgg atgatcgcgg cgaggtcggg gtagcgcggg cgccagccga
     4861 ggagcgtgtg cgccttcgcg gcgtcggcga cgaggtgcga agggtctccg gcgcggcgcg
     4921 gctccatccg cgcctcgatg ggtcggcccg tcacctgccg cgccgtctcg atcacttcga
     4981 gcacagagta gccctgcccg ttgccgaggt tgatgcgctc ggactcgccc ccttcgcgca
     5041 ggtgcttgag ggcgaggatg tgcgcctcgc cgaggtctgc gacgtggatg tagtcgcgca
     5101 ccgcggtgcc gtccttggtc gggtagtcgg cgccgaagac ggagacgtag ggcagcccgc
     5161 ccatcgccgc cgccatcacg ttcggcagca ggtgcgtctc ggggtcgtgc tgctcgccca
     5221 ggttttcg
//
LOCUS       DBNGOH010000279         2688 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_408243, whole genome shotgun sequence.
ACCESSION   DBNGOH010000279 DBNGOH010000000
VERSION     DBNGOH010000279.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2688)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2688)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2688
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_408243"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..278
                     /locus_tag="AC159P_05320"
     CDS             <1..278
                     /locus_tag="AC159P_05320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694906.1"
                     /translation="AAFGDEDVRALSSFVRARLFDGVEHRPASFIRNIRFNLRARRRW
                     RDRLRYYGFLLAPNDGDIVALRLPAPMTFAYYLIRPLRLLTKGDGQH"
     gene            674..1492
                     /locus_tag="AC159P_05325"
     CDS             674..1492
                     /locus_tag="AC159P_05325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694907.1"
                     /translation="MAVLASAPVFAQQQPPPQPARNEDKEARAGADEAPAGDEKAAKE
                     KRTWTLRMSKDTPAAFTLKAKEARLGEITEEIGRLLKTQVFVSPLLTDERVSLEFSGM
                     PLEAVIRMLAPQAYIDYEVGGGEQGPPKALAIYLNALNEKPPSTTEVVKGASEAILIE
                     GDTEEGTGDEEAQKKRDEENPLKVSYARNNLSVRARKQPLTVVLFKIAGELGIPFELR
                     YESPEVVDVDFNNYTVEQAIRGLSPAVRLFYRTDLQTYDVQPLRIVLAAPAVKS"
     gene            1531..1716
                     /locus_tag="AC159P_05330"
     CDS             1531..1716
                     /locus_tag="AC159P_05330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694908.1"
                     /translation="MSTKQESPVAAPQPAHARVPDEQLPEPEVARKRFVEPEVSAPVN
                     VLDATTFFQAATSGGTN"
     gene            1761..1943
                     /locus_tag="AC159P_05335"
     CDS             1761..1943
                     /locus_tag="AC159P_05335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694909.1"
                     /translation="MTEQELPERTPPDLQVEQGGGRGAAPGPVRSAFIEPDISAPVDV
                     LEATTFFQAATSGGTN"
     gene            1952..2140
                     /locus_tag="AC159P_05340"
     CDS             1952..2140
                     /locus_tag="AC159P_05340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694910.1"
                     /translation="MKPSVNMKEELTITENLAEQLDARNVAETGKRCFVEPEISVPVD
                     VLEATTFFQGITSGVTLP"
     gene            2179..>2688
                     /locus_tag="AC159P_05345"
     CDS             2179..>2688
                     /locus_tag="AC159P_05345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694911.1"
                     /translation="MDTLSISARPALRKFYRVAGRSLLVEARDEEAARLVARFFDAWY
                     FAPSEPVAEVCAALRFRSDGPAPSAPRGFEGFELPGGGHCRSDGNVFYITFGDSHVTV
                     TGDCSAEVRVRLGGGLREGSHALANVISYAASAALRRCGVVELHSGGVQDPASGKGAL
                     IVGASGSGKS"
BASE COUNT          491 a          906 c          847 g          444 t
ORIGIN      
        1 gcgcggcgtt cggcgacgaa gacgtgcgcg cgttgtcgtc attcgtccgc gcgcgcctct
       61 tcgacggggt ggagcacagg ccggcgagct tcatccgaaa catccgcttc aacctgcgcg
      121 cgcgccgacg ctggcgcgac cgcctccgct actacggctt cctcctcgcc ccgaacgacg
      181 gcgacatcgt cgccctccgg ctgcccgcgc cgatgacctt cgcgtattac ctcatcaggc
      241 cgttgcgcct gctcacgaag ggcgacggcc aacactgaca gcaggagccg gaatgttcgt
      301 gggggaagaa ccgcgcaacg atggatacgc aatgttggat aaattacaag gcttccgatt
      361 cgccattcat cgttgaaaac gctcgcacgc atgggtgatc cccagtcctg tctgccgtcg
      421 aaatgccccg gcgtgcggcg cctcctaaac gttgtgtacg gtttacgttg acgctcccgc
      481 attatttatg ttagcttccg catgcccgca cttttgccta acgtctgccg ctaatggcaa
      541 ctaaaacaaa gccttagatg gcgcggcgcc ggggttgagc agaccgacag caagtctccg
      601 tggtaggaag gtctctgcaa tcttaatgcg taatcatctt tctcgattgg ggttttccct
      661 gtccgcagtg gtgttggcag tcttggcgtc cgcgcctgtt ttcgcgcaac agcagccgcc
      721 gccacagccc gcgcgcaatg aagacaaaga ggcgcgcgcc ggtgcggacg aagcgccggc
      781 gggcgacgag aaggcggcga aagagaagcg cacgtggacg ctgcggatga gcaaggatac
      841 gcccgccgcg ttcacgctca aggcgaagga ggcgcggctc ggcgagatca ccgaagagat
      901 cgggcggctc ctgaagacgc aggtcttcgt cagcccgctg ctcacggacg agcgcgtctc
      961 gctcgaattc agcgggatgc cgctcgaagc cgtgatccgc atgctcgcgc cgcaggctta
     1021 catcgactac gaggtcggcg gcggcgaaca ggggcctccg aaggcgctcg ccatctacct
     1081 caacgcgctg aacgagaagc cgccctcgac gaccgaggtc gtgaagggcg cctcggaggc
     1141 gatcctcatc gaaggcgaca cggaggaagg caccggcgac gaagaggcgc agaagaagcg
     1201 cgacgaggag aatccgctca aggtctctta cgcgcgcaac aacctcagcg tccgcgcgcg
     1261 caaacagccg ctgacggtcg tcctcttcaa gatcgccggc gaactcggca tccccttcga
     1321 gctgcgctac gagtcgcccg aggtggtgga cgtggacttc aacaactaca ccgtcgagca
     1381 ggccatccgc ggtctgtctc ccgccgtgcg cctgttctac cgcaccgact tgcagacgta
     1441 cgacgtgcag ccgctccgca tcgtgctggc ggcgcccgcg gtcaaatcct gagctttcat
     1501 tcccacctcg ctgtaaggag gcacgcgcct gtgagcacaa agcaagagtc cccggtcgcc
     1561 gcgccgcagc cggcgcacgc gcgcgtcccc gacgaacaac ttcccgaacc cgaagtcgca
     1621 cggaagcggt tcgtcgaacc cgaagtttcc gcacccgtga acgtcctcga tgccactacg
     1681 ttctttcagg ccgcgaccag cggcggcacg aattgacaac gacacgcaat cgccgaatcc
     1741 attgaggagg ttggatcgtt ttgaccgaac aggaactccc cgaacgcacg ccgcccgact
     1801 tgcaggtcga acagggcggg gggcggggcg cggctcccgg ccctgtcagg agcgctttca
     1861 tcgagccgga catctctgcc ccggttgacg tactcgaagc caccacgttc ttccaggccg
     1921 cgaccagcgg cggtactaac taagcgaaag gatgaagcca tcggtgaaca tgaaggaaga
     1981 gttgacgatc acggagaacc tcgctgagca gttagacgcg cgcaacgttg ccgagaccgg
     2041 gaagaggtgc ttcgtcgagc cggagatttc ggtgcccgta gacgtgctcg aagccacgac
     2101 cttcttccag ggcatcacga gcggcgtgac gctcccctga cagaccggaa gggagaacac
     2161 gttcccggcc ctgctgcaat ggacaccctt tccatctctg cccgaccggc gctccgtaag
     2221 ttttacaggg tcgccggtcg ttcccttttg gtcgaggcgc gcgacgaaga ggcggcgcgc
     2281 ctcgtcgcgc gcttcttcga cgcctggtat ttcgccccgt ccgagccggt cgcagaggtc
     2341 tgcgccgccc tccgcttccg aagcgacggc cccgcgccct cggcgccgcg cggcttcgaa
     2401 gggttcgagc tgcccggcgg cggccactgc cgctccgacg gcaacgtctt ctacatcacg
     2461 ttcggcgact cgcacgtcac cgtcacgggc gactgctccg cggaggtgcg cgtgcggctc
     2521 ggcggcggcc tgcgcgaagg ctcgcacgcg ctcgccaacg tcatctctta cgcggcctcg
     2581 gcggcgctcc gccggtgcgg cgtggtcgag ctgcacagcg gcggcgtgca agaccccgcg
     2641 agcggcaagg gcgcgctcat cgtcggcgct tcgggcagcg gcaagtcc
//
LOCUS       DBNGOH010000280         3652 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4074371, whole genome shotgun sequence.
ACCESSION   DBNGOH010000280 DBNGOH010000000
VERSION     DBNGOH010000280.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3652)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3652)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3652
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4074371"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            332..751
                     /locus_tag="AC159P_05350"
     CDS             332..751
                     /locus_tag="AC159P_05350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694912.1"
                     /translation="MAATEKQPSVSAAELLRRAAVAEDAVEASGPSLVLHRTVFVEEA
                     KRDGRGTSVVVRRRVETWRGGASRIRLRRLYDERGELLAGEWTKSDGASAVYRRGSPP
                     VERGAAASGKELLDAGEVWRIEPSARSFDALGFGATR"
     gene            complement(1087..1494)
                     /locus_tag="AC159P_05355"
     CDS             complement(1087..1494)
                     /locus_tag="AC159P_05355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694913.1"
                     /translation="MVHGARGVALDLRVHAVAFGEPRREVGAQLRVRGDAPRADVDLA
                     LGEIGLRALLLLPPPLQSLGDRAYFDLDRRVVQDWAEGAQDSFLAPLGLDERLDLEGA
                     SCGSPHADLLAEIRFDPVQQIRRLDFQLGGSDR"
     gene            complement(1470..1592)
                     /locus_tag="AC159P_05360"
     CDS             complement(1470..1592)
                     /locus_tag="AC159P_05360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694914.1"
                     /translation="MARASGLSARASAGVNLPASLFSAEAWSNACREWFMALAA"
     gene            1593..2018
                     /locus_tag="AC159P_05365"
     CDS             1593..2018
                     /locus_tag="AC159P_05365"
                     /inference="COORDINATES: protein motif:HMM:NF051698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HIR3694915.1"
                     /translation="MALDDPRARGRLPARDGGAARTTRRRVRRGRRRRGRASGGRDGS
                     LGRNALRGFGLSQLDLAVRRQFALGERARLQLRAEVFNVFNHPNFGDPVADLGSRLFG
                     QSTQTLARSLGTGGVNGGLSPLYQVGGPRSVQLALRLGF"
     gene            2051..2620
                     /locus_tag="AC159P_05370"
     CDS             2051..2620
                     /locus_tag="AC159P_05370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694916.1"
                     /translation="MTRTRKTGGGATRKINVDAPAALLLSLLLLCGLTPRQALSHADE
                     VKGSAVAASASLPHASSNLNARDGRVRNRKTFGQLPASFELNAGQADARVKFLSRGSR
                     HTLLLGADGASLALRTGSEERGGAVAVPRRRVRGETASDAGGHARPLQLLRLKFVGAN
                     PRAAPRAAIAPISSAAAGGEGVRLMAICG"
     gene            2872..>3652
                     /locus_tag="AC159P_05375"
     CDS             2872..>3652
                     /locus_tag="AC159P_05375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694917.1"
                     /translation="MIRTRSQAFILPFLLLLLASPAAAAQEPCFTAEARALAERSAKV
                     YQEPDPGYDPVLGYNPANGPRVGAPKVDEAGLAAPINCVANTKPEKGSGTTPKFHCSV
                     PGVVDADGDLVRYKVKPHFKGQARDRRNGEIYGEFLSSRFSRALGFFADDEWVADVTC
                     PDCRKSLTGSFQGASYQPFQPAAGIELPLGKGIDVKCSDKDSAPLAATLQKLVQGGAP
                     RAEIDAFKLWLAFIDHGDTKTDNHKLSCLKWTKGADNTLVCE"
BASE COUNT          590 a         1270 c         1268 g          524 t
ORIGIN      
        1 cggcccgcgg tgcggtagat tcgacgccga atccggcagg actcggaggg cggggacaag
       61 ccccgcccct acaggccggc aacggtctct tggcatatga tggcgacctc gtttgatgtg
      121 tgcgaagaca cgggaagact cttcggcgct ccgcgctcca tggtctctgt ccatcttcta
      181 aacaccgccc gcgtcttttc cggggcagaa aaatgcaggg gcgggcttcg gcgcgacact
      241 gttgcggagt gcgccctccc tccgtaatca cgtaaactcc gtgacgagcg atagacgagg
      301 acgtggacgc cctccttcgg gcgcatggtg catggcggcg accgagaagc agccgtcggt
      361 ctccgccgcc gagctgctgc ggcgcgcggc ggtggcggaa gacgcggtcg aagcctccgg
      421 cccgagcctc gtgctccatc gcacggtctt cgtcgaagag gcgaagcgcg acgggcgcgg
      481 cacgagcgtc gtcgtgcgcc ggcgcgtcga gacgtggcgc ggcggcgcga gccgcatcag
      541 gctgcggcgg ctctacgacg agcgcggcga gctgctcgcg ggcgagtgga cgaagtccga
      601 cggcgcgagc gccgtctacc gccgcggctc gccgcccgtc gagcgcggcg cagcggcgtc
      661 ggggaaagaa ctgctcgacg cgggcgaggt ctggcgcatc gagccttcgg cccgcagctt
      721 cgacgcgctc ggcttcgggg cgacgcgttg aaggccgatg acaggctcgg cgcttacgtg
      781 ctggcttacg agtcggcggc gcgcgccgac ggtctcgaag gtcttattag tgcggagctg
      841 tggctcaaca agtcggacct gcgcgcctac aggatgacgc tcgtcgtgcg gcgcggcggc
      901 gaagtggtcg agtaccggct cgtcgaaggc ggcttcgagc gcaagcgcgc ggaggaggtg
      961 ccgcccggcg tctaccggct cgaacccgag ctgctcggga cgactcaagc gacgaaggat
     1021 gaaggcggcg cgatgaaggc tcagcgcggt gaaggcgcac ggccctcgtc gctcatcccg
     1081 cagccctcag cggtcgcttc cgccgagctg gaagtcgagg cggcgtatct gctgaaccgg
     1141 atcaaagcga atctcggcga gcaagtcagc atggggcgaa ccacaggagg cgccctcaag
     1201 gtcgaggcgc tcgtcgagtc cgaggggcgc aaggaagcta tcttgcgcgc cctcggccca
     1261 gtcctgaaca acccggcggt cgaggtcgaa gtaagcacgg tcgccgaggc tctggagcgg
     1321 cggcggcaga aggagcaagg cgcgcagacc gatctcgccg agcgcgaggt caacgtccgc
     1381 gcggggcgcg tccccgcgga cgcggagctg cgcgcctact tcgcggcgcg gctcgccgaa
     1441 ggcgaccgcg tggacgcgga gatcaagcgc tacgccgcga gcgccatgaa ccactcgcgg
     1501 caggcgttgc tccacgcctc ggcgctgaag aggctggccg gcaggttcac gcccgcagag
     1561 gcccgcgcgc tcaacccgga cgcgcgcgcc aagtggctct cgatgatccg cgagcacgcg
     1621 ggcgcttacc ggcgcgagac ggcggcgctg cgcgcacaac tcggcgccgt gttcggcgcg
     1681 ggcgacgaag gcgcgggcga gcctcgggcg gcagggacgg ctcgctcgga cgcaacgcgc
     1741 tgcgcggctt cggcctctcg cagctcgacc tcgccgtgcg ccgccagttc gcgctcggcg
     1801 agcgcgcgcg cctccaactg cgcgcagagg tcttcaacgt tttcaaccac ccgaacttcg
     1861 gcgacccggt cgcagacctc ggcagccgcc tcttcggcca gagcacgcag acgctcgcgc
     1921 gcagcctcgg caccggcggc gtcaacggcg gactcagccc gctctatcag gtcggcggcc
     1981 cgcgctccgt acagctcgcg ctgcggctcg gcttctgaca ctcacaccgg acacgaggag
     2041 ggagcgagtg ttgacgcgga cgcgaaagac gggcggcggc gcgacacgga agataaacgt
     2101 tgacgcgccc gcggcgctcc tgctctcgct gttgttgctc tgcggcctga cgccgcgcca
     2161 agcgctttcg cacgccgacg aagtgaaggg aagcgccgtc gccgcttcgg cttcgctccc
     2221 tcatgcgtca tcgaatctca acgcgcggga cgggcgcgtt cgaaatcgaa aaaccttcgg
     2281 ccagttgccg gcgagcttcg agctgaacgc cgggcaggcg gacgcgcgcg tcaagttcct
     2341 gtcgcgcggc agccgccaca cgctactgct cggcgcggac ggggcctcgc tcgcgctccg
     2401 gacgggcagt gaggagaggg ggggcgccgt ggccgtgccg cgccgacggg tacgtgggga
     2461 gactgcctcc gacgccggcg gccacgcgcg ccccctccaa ctcctgcgcc tgaagttcgt
     2521 cggcgccaac ccccgcgccg ccccacgggc tgcaatcgcc cccatttcaa gcgctgctgc
     2581 cggtggagaa ggagtgaggc tgatggcaat ttgcggctga gcgtagcgga agttcggatt
     2641 caacgcagag ggcgcagagg gagcgcagag ggcgcagaga agactgattg cttcctctgc
     2701 gtcctctgcg ctccctctgc gccctctgcg ttgacatgaa ccgcgctaca ctcaaccgcc
     2761 aaccgctcta gtggtgtgtg ctaaaccgcg cttgcctagt ggcgtgcgtc tcggctactt
     2821 ttgctctacg cattttgccg aagagactcc tcgtcaagaa agggcgcgtc catgattcgt
     2881 actcggtcgc aagcgttcat cctccccttc ctccttctcc tcctcgcgtc gccggcggcg
     2941 gcggcgcagg aaccctgctt caccgcggaa gcgcgtgcgc tcgccgagcg gagcgcgaag
     3001 gtctatcagg agcctgaccc cggctacgac cccgtgctcg gttacaaccc cgcgaacggc
     3061 ccgcgcgtcg gcgccccgaa ggtggacgag gccggactcg ccgcgcccat caactgcgtc
     3121 gccaacacga agcccgagaa gggcagcggc accacgccga agttccactg ctctgtgccg
     3181 ggcgtcgtgg acgcggacgg cgacctcgtc cgctacaaag tgaagcccca cttcaagggg
     3241 caggcgcgcg accggcgcaa cggcgaaatc tacggcgagt tcctctcgtc gcgcttctcg
     3301 cgggcgctcg gcttcttcgc cgacgacgag tgggtcgccg acgtgacctg ccccgactgc
     3361 cggaagagcc tgaccggcag ctttcagggc gcgagctatc agcccttcca gcccgccgcg
     3421 ggcatcgagc ttccgctcgg caaaggcatc gacgtgaagt gcagcgacaa ggactcggcg
     3481 cccctcgccg cgacgctcca gaagctcgtt cagggcggcg cgccccgcgc cgagatcgac
     3541 gccttcaagc tctggctcgc cttcatcgac cacggcgaca ccaagaccga caaccacaag
     3601 ctctcctgcc tcaagtggac gaagggcgcc gacaacacgc tcgtctgcga ag
//
LOCUS       DBNGOH010000281         5232 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3157520, whole genome shotgun sequence.
ACCESSION   DBNGOH010000281 DBNGOH010000000
VERSION     DBNGOH010000281.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5232)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5232)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5232
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3157520"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2258
                     /locus_tag="AC159P_05380"
     CDS             <1..2258
                     /locus_tag="AC159P_05380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694918.1"
                     /translation="RIGIYAMAAAGYDPQAFVSMWNRLTDTKGKTGGWLSDLFGTTRP
                     ESRRLREILKGVSTLPQQCIAARSAVSPEEFKRWQAAVVSLPTAARAESLHAVLSKTK
                     LDPPLRGDINHLRFSPDGKYLLAQDDSGINVLTREPLAPAFRIEAPEARQARFTPDSK
                     RVVFHSAALRVEAWDVEAQKADGVREMYVREGCMQTELSPDAKTLACLDTNYALALYD
                     VPTGAQLFQKKDFFKPDLADLVNIILLGVFRGDGDFADHAPDIEWVSMEFSPDARHFA
                     AGQRSTNFTALATVHTEVSAVVYDVAEKETVPLKGQLKKMLSGRFAFVAPDRIVAVNP
                     EDWRKSAVLSFPAGEVVEPHEIGGTPDGVAGGNYILLRPIQHYAVGVYDLASRKIILA
                     NKTRAFDIHGDVFASERTNGELGLYRVAGANELTAKVLLPRNTLGRLRAASVSPDFKW
                     LAVSERSRGAVWDLTKGERVFHVRGFRGAHFGEDGTLYADFPTLGDTKRNVAHLKLST
                     RDALEGAQVEDSWLVEQHGPYVVVRKPLKKGGDPWENIALEVLDARTATRLWSRDFPK
                     EAPRVWVDPVGDMVVLGWDAKSNAAKAELKNDPKLTQRLSAMKEKEGDYLLQTLDART
                     GRQTGALLVETGKGSFRVAGVAAAGDLLLISDTENRVLVYSLADGEQKGRFFGGSPSV
                     SKAAGLLSVENERGQLAVYDLATMAKRDQFTFTSPVSLTRFSPDGKRLFVLTANQTAY
                     VLNMAPTAQTQ"
     gene            complement(2385..2942)
                     /locus_tag="AC159P_05385"
     CDS             complement(2385..2942)
                     /locus_tag="AC159P_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011142343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HIR3694919.1"
                     /translation="MTVRISPLLTAADLELMPDDGNRYELVEGELLVTRAPGVPHQRV
                     ITNLIKLIGHYLDRNPLGEILTVPGVIFDEYNSVIPDLVFLTNEQTERAGPHIHEAPE
                     LAVEIVSPGRENARRDRVVKLQVYSKFKVKEYWVADPEARTLEVYRLSEGALTLAATL
                     KGDDEITSPLLPGFGCKVSEIFGSR"
     gene            3085..5211
                     /gene="uvrC"
                     /locus_tag="AC159P_05390"
     CDS             3085..5211
                     /gene="uvrC"
                     /locus_tag="AC159P_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HIR3694920.1"
                     /translation="MTLEEKLRNLPTSPGVYLHKDEAGKIIYVGKAKNLRNRVRSYFQ
                     SGRGHDRKTRELVLRISDLEFIVTDTEVEALVLESNLIKRHKPRYNVLLKDDKQYPHL
                     KLTVNEAFPRVMITRRVQRDGALYFGPYLPASLARRTIDLINRTFQLRTCDIEIDGKL
                     PRPCLEYHIKRCLGPCVKGLCTPEEYQESVRDVRLFLEGRNRELADEYEGRMARAAEE
                     MRYEMAAKYRDLRKVVLAVSEQQKMATTAERDVDIFGYYREGPRLALQLFTMREGKMV
                     GRREFFWEDLAAEEFDPAKFLGEVLTQYYSTDYVPREVNVPADFEDRELLEKALTERK
                     GRRVRILDPQRGEKRDMIALVEKNARIAFEQRFRVLKPDMERVLEELQEVLELPRFPA
                     RIESFDVSNISGAENVAAMVVCENGKMNRQEYRKFKIKTVEGANDPASMGEAVFRRYR
                     RQLDEAKPLPDLVMIDGGKTQLAAAAAAMRELDLEAVPVVGVVKPPRRHNEVSHLLMK
                     GREHAPVYLDSHSPVLRLIQMIRDETHRSAVTYHRKRRELRDFTSELTAIPGVGEKRK
                     NRLLRNLGSIQRVSTASVAELTPFVGRKTAEDIFEHFARQRALAEGGTRDAAEADLSS
                     STAAADATVMTDEMIGDEHVETRLVDPEGDAEDLQPIRSVDHEGNPRRRRKSTRGRGE
                     RSTNPHGIKREKLSEGNDGAGGARES"
BASE COUNT          951 a         1831 c         1661 g          789 t
ORIGIN      
        1 accgcatcgg catctacgcg atggccgccg cgggctacga cccgcaggcc ttcgtctcga
       61 tgtggaaccg cctgaccgac acgaagggca agaccggcgg ctggctctcc gacctcttcg
      121 gcacgacccg ccccgagtcg cgccgcctgc gcgagatatt gaagggcgtc tccacgctgc
      181 cccagcagtg catcgccgcg cgctcggccg tcagcccgga ggagttcaag cgctggcagg
      241 cggcggtggt gagcctcccg acggcggcgc gcgccgagtc gctccacgcc gtgctctcga
      301 agacgaagct cgacccgccg ctgcgcggcg acatcaacca cctgcgcttc agccccgacg
      361 gcaagtacct tttggcgcag gacgactcgg gcatcaacgt cctgacgcgc gagccgctcg
      421 cgcccgcctt ccgcatcgag gcgcccgagg cgcgccaggc gcgcttcacg cccgactcga
      481 agcgcgtcgt cttccacagc gcggccctgc gcgtcgaggc gtgggacgtg gaggcgcaga
      541 aggccgacgg cgtgcgcgag atgtacgtcc gcgagggctg catgcagacg gagctatcgc
      601 ccgacgcgaa gacgctcgcc tgcctcgaca ccaactacgc gctcgcgctc tacgacgtgc
      661 ccaccggcgc gcagctcttc cagaagaagg acttcttcaa gcccgacctc gccgatctcg
      721 tcaacatcat cctgctcggc gtcttccgcg gcgacggcga cttcgccgac cacgcgcccg
      781 acatcgagtg ggtgagcatg gagttctcgc ccgacgcgcg ccacttcgcc gccgggcagc
      841 ggagcaccaa cttcaccgcg ctcgccaccg tccacaccga agtctcggcg gtcgtctacg
      901 acgtggcgga gaaggagacc gtgccgctca agggccagtt gaagaagatg ctgtcgggcc
      961 gcttcgcctt cgtcgcgccc gaccgcatcg tcgccgtcaa cccggaggac tggcgcaagt
     1021 ccgccgtcct cagcttcccc gcgggcgagg tcgtggagcc gcacgagatc ggcggcacgc
     1081 ccgacggggt cgccggcggc aactacatcc tgctccggcc catccagcac tacgcggtcg
     1141 gcgtctacga cctcgcgtcc aggaagatca tcctcgccaa caagacgcgc gccttcgaca
     1201 tccacggcga cgtgttcgcc tccgagcgca ccaacggcga actcggcctc taccgcgtcg
     1261 cgggcgcgaa cgagctgacc gccaaagtcc tgctcccgcg caacacactg ggccgcctgc
     1321 gcgcggcctc cgtctcgccc gacttcaagt ggctcgccgt ctccgagcgc tcgcgcggcg
     1381 ccgtctggga cttgaccaaa ggcgagcgcg tcttccacgt ccgcggcttc cgcggcgcgc
     1441 acttcggcga agacggcacg ctctacgcgg acttccccac gctcggcgac acgaagcgca
     1501 acgtggccca cctcaagctc tcgacgcgcg acgcgctcga aggcgcacag gtcgaagact
     1561 cctggctcgt cgagcagcac ggcccttacg tcgtcgtgcg caagcccttg aagaagggcg
     1621 gcgatccttg ggagaacatc gcgctcgaag tcctcgacgc gcgcacggcg acgcgcctct
     1681 ggtcgcgcga cttcccgaag gaggcgccgc gcgtgtgggt cgatccggtc ggcgacatgg
     1741 tggtgctcgg gtgggacgcc aagtcgaacg cggcgaaggc cgagcttaag aacgacccga
     1801 agctcacgca gcggctctcg gcgatgaagg agaaggaggg cgactacctc ttgcagacgc
     1861 tcgacgcgcg gacgggcagg cagacgggcg cgctcctcgt cgagaccggc aaaggctctt
     1921 tccgcgtcgc gggcgtcgcc gcggcgggcg acctgctgct catctccgac acggagaacc
     1981 gcgtcctcgt ctactcgctc gccgacggcg agcagaaggg gcgcttcttc ggcggcagcc
     2041 cttcggtctc gaaggccgcc ggcctcctca gcgtcgagaa cgagcgcggc cagctcgccg
     2101 tctacgacct cgccacgatg gccaagcgcg accagttcac cttcaccagc cccgtctccc
     2161 tcacgcgctt cagccccgac ggcaagcgcc tcttcgtcct caccgccaac cagaccgcct
     2221 acgtcctcaa catggcaccg accgcccaaa cgcagtaagg caagggaaaa gggaaggggg
     2281 aaagggggaa atggcgaaat ggcgaaaagg gaagaaagct cgcacgtccc gagtcattct
     2341 tcccttttcg ccttttcccc ttttaacctt ttcccccttc ctcttcaccg gctgccgaaa
     2401 atctcgctca ccttgcaacc gaatcccggc agcaacggcg acgtgatttc atcgtccccc
     2461 ttcagcgtcg ccgcgagcgt caatgcgcct tcactcagac gatagacttc caacgttcgc
     2521 gcctcggggt cggccaccca gtattctttg actttgaatt tgctgtagac ctgtagcttc
     2581 acgacgcggt cgcgccgcgc gttctccctg cccggcgaga cgatctcgac ggccaactcc
     2641 ggcgcctcgt ggatgtgcgg cccggcgcgc tccgtctgtt cattggtgag gaacacgagg
     2701 tcggggatga cgctgttgta ctcgtcgaag atgacgccgg gcaccgtaag aatctccccc
     2761 agcgggttgc ggtcgaggta gtggccgatg agcttgatca aattggtgat gacgcgttgg
     2821 tgcgggacgc cgggtgcgcg ggtcacgagc agttctcctt cgacgagttc gtagcggttg
     2881 ccgtcgtcgg gcatgagttc gaggtcggcg gcggttaaga gcggcgagat tctgactgtc
     2941 atgggaacct cctgactcgt gcgctgtggg ccgaacagta gcacagccga cagcggcccg
     3001 ccacgggttt tcacatgcaa cgggcgttgc agtagactat cttcagtacc cccaaagatt
     3061 aggacggcga gcagggaaaa agcgttgacg ctcgaagaga aactacggaa tctgcccacg
     3121 tcgcccggcg tctacctgca caaggacgag gcgggcaaga tcatctacgt cggcaaggcg
     3181 aagaacctcc gcaaccgcgt ccgctcctac ttccagtcgg gccgcgggca cgaccgcaag
     3241 acgcgcgagc tggtgctccg catcagcgac ctcgaattca tcgtcaccga cacagaggtc
     3301 gaagcgctcg tcctcgaatc gaacctcatc aagcggcaca agccgcgcta caacgtcctc
     3361 ttgaaggacg acaagcagta cccgcacctc aagctcaccg tcaacgaggc gttcccgcgc
     3421 gtgatgatca cgcggcgcgt gcagcgcgac ggcgcgctct acttcgggcc gtacctgccg
     3481 gcctctctgg cgcggcgcac catcgacctc atcaaccgca cgttccagct ccgcacctgc
     3541 gacatcgaga tcgacggcaa gctcccgcgc ccctgcctcg aataccacat caagcgctgc
     3601 ctcggcccct gcgtcaaggg cctgtgcacg cccgaagagt atcaggagtc cgtgcgcgac
     3661 gtgcggcttt ttctagaagg tagaaacagg gaactcgcgg acgagtacga ggggcggatg
     3721 gcgcgagccg ccgaggagat gcgttacgag atggccgcga agtaccgcga cctgcgcaaa
     3781 gttgtgctcg ccgtctccga gcagcagaag atggcgacca ccgccgagcg cgacgtggac
     3841 atcttcggct actaccgcga ggggccgcgc ctcgcgctgc aactcttcac catgcgcgag
     3901 gggaagatgg tgggccgccg cgagttcttc tgggaagacc tcgcggcgga ggagttcgac
     3961 ccggcgaagt ttttgggcga ggtgctgacg cagtattact cgaccgacta cgtgccgcgc
     4021 gaggtcaacg tgcccgcgga cttcgaagac cgcgagcttc tggagaaggc tttgacggag
     4081 aggaaggggc ggcgtgtccg catcctcgac ccgcagcgcg gcgagaagcg cgacatgatc
     4141 gcgctcgtcg agaagaacgc gcgcatcgcc ttcgagcaac gcttccgcgt cttgaagccc
     4201 gacatggagc gcgtgctcga agagttgcag gaggtgcttg agctgccgcg cttcccggcg
     4261 cgcatcgagt cgttcgacgt gtcgaacatc tcgggcgcgg agaacgtcgc ggcgatggtc
     4321 gtctgcgaga acgggaagat gaaccggcag gagtaccgca agttcaagat caagacggtc
     4381 gaaggcgcga acgacccggc ctcgatgggc gaggccgtct tccgtcgcta ccgccgccag
     4441 ctcgacgaag cgaagccctt gcccgacctc gtcatgattg acggcgggaa gacgcagctc
     4501 gccgccgccg ccgccgcgat gcgcgaactc gacctcgaag ccgtccccgt ggtcggcgtc
     4561 gtcaagccgc cgcgccgcca caacgaagtc tcgcacctcc tgatgaaggg gcgcgagcac
     4621 gcgcccgtct acctcgactc gcactcgccc gtgctgcgcc tcatccagat gatccgcgac
     4681 gagacgcacc gcagcgccgt cacctaccac cgcaagcggc gcgagctgcg cgacttcacc
     4741 tcggagctga cggccatccc cggcgtcggc gagaagcgca agaatcgcct gctgagaaac
     4801 ctcggctcga tccagcgcgt ctcgaccgcc tcggtcgccg agctgacgcc cttcgtcggc
     4861 aggaagaccg ccgaagacat cttcgagcac ttcgcgcgcc agcgcgcgct cgccgaaggc
     4921 ggcacgaggg acgccgcgga agcagacctt tcgtcatcga ccgccgccgc cgacgcgacc
     4981 gtgatgacgg acgaaatgat cggcgacgag cacgtcgaga cgcgcctcgt cgatcccgaa
     5041 ggcgacgccg aagatctcca gcccatccgc tccgtcgatc acgaaggcaa cccgcgccgc
     5101 cgccgcaagt ccacgcgcgg gcgcggcgag cgctcgacca acccgcacgg catcaagagg
     5161 gagaagttgt cggaagggaa cgacggggcg ggcggggcga gagagagttg agcacgtctt
     5221 gtcgcaatcg tc
//
LOCUS       DBNGOH010000282         2540 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3891982, whole genome shotgun sequence.
ACCESSION   DBNGOH010000282 DBNGOH010000000
VERSION     DBNGOH010000282.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2540)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2540)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2540
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3891982"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="AC159P_05395"
     CDS             <1..189
                     /locus_tag="AC159P_05395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694921.1"
                     /translation="PYICHERSHSFANRCDFELCLCVSMVNWIISVRAREADLRPLRI
                     ENSRAPHPPAVVNKPRRD"
     gene            640..>2540
                     /locus_tag="AC159P_05400"
     CDS             640..>2540
                     /locus_tag="AC159P_05400"
                     /inference="COORDINATES: protein motif:HMM:NF013106.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HIR3694922.1"
                     /translation="MAIEITSVREARRLPRLRSAAASLRERAVCPATRTRLVRFFCAS
                     LIVVSLASLAVVVYFYFHYSNVVDQRLASGYLTSRAGLYAAPRVLRAGQHISRERLTD
                     ILRRAGYVDSEASRVWSGQFAVAGDAVEIFPRTSGANTEASVYEVVRVEFDRAGRIKG
                     IRGDGAALDSYALEPEPLTNDAGVKTDEHETLAYTDIPPVLVRAILSIEDRRFFEHGG
                     IDPVGLARAAVGWGDADRTQKQGGSTITQQLVKNTYLTPERTLRRKFHEAIIARVIER
                     RMSKEDILALYCNEIYLGQRGSRGVRGVEQAARVFFGKELRDLSLAEAATVAGMIQSP
                     GRYAPDRHPEQAKVRRNTVLAAMLRDGAITREEAEAAAREPVAVAQAETADALAPHFI
                     DYVNRRVAERHAGGDVADESNLRIHTTIDIELQRLAELAVRGQLERLHKVFKGKKQPQ
                     AALVALDPKTGHVLAMVGGGSYAESQLNRATDARRQPGSVFKPIVYAAAIEGGISPLS
                     VSLDAPREFAYDHRAKYRPANYGGGYSHRDVTLRTALVRSLNVVTVETAMRVGLGRVS
                     ALAGRMGLPEPEAYPALALGSNEATPLEVAGAYTAFANNGRAVRPTAIARVEDGAQPQ
                     SFAAAVPAA"
BASE COUNT          433 a          914 c          801 g          392 t
ORIGIN      
        1 ccctacatct gtcacgaacg gtcacactca ttcgccaacc gctgtgattt tgaactgtgc
       61 ctctgtgtct ctatggtgaa ttggattatt tcggtacgcg cgcgcgaggc cgacctccgg
      121 cccctgcgta ttgaaaacag ccgcgcccct catcctcctg ccgtcgtcaa caaaccccgc
      181 cgcgattgat tcgcttctcg aactctctgt agaaacggac tttgaatgat ttctcgacag
      241 caaccttcgt gcgcgcacgc ggtcggcacg gtcattgcac acgaagccct ttgagaacgg
      301 attagtgaac gtggggggat acgcaagatg agagaagtag ccgacgggtt catgcaactg
      361 ctcgccatca tcatggtgtt gataacgttg gcggtctcga ccgccgcgtt cgggcagatg
      421 ctcggttgag gcgttgtcgt gaacaagggg ctgtagttcg ggagacgaaa gccactttac
      481 ctcccgtcat tcgccctttg ctcgaagcaa gaaaactttg acgattgaac atatctctat
      541 agcacacaga cggcacgcgg ggcaggccga cggcggggca cacgcacatc acgtcgcgcg
      601 cacggcggga cgagtgtcgc cctgcggcaa ccgaacgaca tggccatcga aatcacatcc
      661 gtccgcgaag cgcgccgcct gccgcgactc aggagcgccg cggccagcct ccgcgaacgc
      721 gccgtctgcc ccgcgacgcg gacacgcctc gtccgcttct tctgcgcttc gctgatcgtc
      781 gtctcgctcg cctccctcgc cgtcgtcgtc tacttctatt ttcactactc gaacgtcgtc
      841 gatcagcgac tcgcctcggg ctacctgacg agccgcgccg ggctgtacgc cgcgccgcgc
      901 gtgctgcgcg ccggtcagca catctcgcgc gagcggctga cggacatcct gcgccgcgcg
      961 ggctacgtcg attcggaagc gagccgcgtg tggagcggcc agttcgccgt cgcgggcgac
     1021 gcggtcgaaa tcttcccgcg cacgtcgggc gcgaacaccg aagcctcggt ctacgaagtc
     1081 gtgcgcgtcg agttcgaccg cgccgggcgc atcaagggga tcaggggcga cggcgccgcg
     1141 ctcgactctt acgcgctcga acccgagccg ctgacgaacg acgcgggcgt caagaccgac
     1201 gagcacgaga cgctcgccta cacggacatc ccgcccgtgc tcgtccgcgc catcctctcg
     1261 atcgaagacc ggcgcttctt cgagcacggc ggcatcgacc cggtcggcct cgcgcgcgcc
     1321 gccgtcggct ggggcgacgc agaccggacg cagaagcaag gcggctcgac catcacgcag
     1381 cagctcgtca agaacaccta cctcacgccc gagcgcacgc tgcgccgcaa gttccacgag
     1441 gccatcatcg cgcgcgtcat cgagcggcgg atgtccaaag aggacatcct cgcgctctac
     1501 tgcaacgaga tttatctcgg ccagcgcggc agccgcggcg tgcgcggcgt cgagcaggcg
     1561 gcgcgcgtct tcttcggcaa ggagctgcgt gacctctcgc tcgcagaggc cgcgaccgtc
     1621 gccggcatga tccagagtcc cggtcgctac gcgcccgacc gccacccaga gcaggcgaaa
     1681 gtccgccgca acaccgtgct ggcggcgatg ctgcgtgacg gcgcgatcac gcgcgaagag
     1741 gccgaggccg ccgcgcgcga acccgtcgcg gtggcgcagg ccgaaacagc ggacgccctc
     1801 gccccgcact tcatcgacta cgtcaaccgc cgcgtcgccg agcgtcacgc cggcggcgac
     1861 gtcgccgacg aaagcaacct gcgcatccac acgacaattg acatcgaact acagcgcctg
     1921 gccgagctgg ccgtgcgcgg gcagctcgaa cgtctgcaca aggtcttcaa agggaagaag
     1981 cagccgcagg ccgcgctcgt cgcgctcgac ccgaagacgg gccacgtgct ggcgatggtc
     2041 ggcggcggct cgtacgccga gtcgcagctc aaccgcgcga ccgacgcgcg ccgccagccc
     2101 ggctcggtct tcaagcccat cgtctacgcg gcggccatcg agggcggcat ctcgcccctc
     2161 tcggtctcgc tcgacgcgcc gcgcgaattt gcttacgacc accgcgcgaa gtaccgcccg
     2221 gccaactacg gcggcggcta ctcgcatcgc gacgtgaccc tgcgcacggc gctcgtccgc
     2281 tcgctcaacg tcgtgacggt cgagacggcg atgcgcgtcg gcttgggccg cgtgtcggcg
     2341 ctcgcgggcc gcatggggtt gccggagccg gaagcttatc cggcgctcgc gctcgggtcg
     2401 aacgaggcga cgccgctcga agtcgcgggc gcctacacgg ccttcgccaa caacggtcgc
     2461 gccgtgcgcc ccacggccat cgcgcgcgtc gaagacggcg cgcaacccca gtcgttcgcc
     2521 gccgcggtgc ccgccgccga
//
LOCUS       DBNGOH010000283         2704 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3708257, whole genome shotgun sequence.
ACCESSION   DBNGOH010000283 DBNGOH010000000
VERSION     DBNGOH010000283.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2704)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2704)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2704
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3708257"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..640)
                     /locus_tag="AC159P_05405"
     CDS             complement(<1..640)
                     /locus_tag="AC159P_05405"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024258.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HIR3694923.1"
                     /translation="MPLSTAFKSKLGKITVAAFAVACALALWAFWLEPASLGVRRVSL
                     KVPAWRAEHGGMKVAVLTDLHVGSPHVGPEKLRRVVELTNEEQPDLVVILGDLVIQGV
                     AGGEFVTPERIAEELKNLKAPHGVVAVLGNHDWWLDGPRVRTALKAAGVTVLENQSVR
                     VERGGRAFHVAGVADMWTRTADVSASLKGVGPDEEPVLLLTHNPDIFPDVPPR"
     gene            complement(695..1516)
                     /locus_tag="AC159P_05410"
     CDS             complement(695..1516)
                     /locus_tag="AC159P_05410"
                     /inference="COORDINATES: protein motif:HMM:NF024869.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HIR3694924.1"
                     /translation="MGAGEDSSTPERLTRALARVLGEGAVAALATVISAPHSVGAKIL
                     FEDDGGARAGTTGDAAFDEAIAGHAAAFLASRAEARTLRVEEFAPALEAWRGARVLFE
                     RVAPEPHVVVCGAGHVGAALARLAQSIGYRVTLIDDRAEFVARARFPEEGIALVAASD
                     WTGPVRETVGTGRGVAVAVVTRGHNEDEECMRAVLAARPDYVGMIGSRRRTNIVLARL
                     RESGFAEDALREVRAPIGLDLGAVTPEEVALAVLAEIVAHRRGGKGLPLSAWRRA"
     gene            complement(1524..1841)
                     /locus_tag="AC159P_05415"
     CDS             complement(1524..1841)
                     /locus_tag="AC159P_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018313361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF962 domain-containing protein"
                     /protein_id="HIR3694925.1"
                     /translation="MPTSFIEEYKAKHQHPLNHLTHAIGIPMIVVSLPLFFFSWRWAL
                     ALFVVGWVFQFIGHAIEGNRPAFFGNPIYLLVGPLWLVRRALAAIGLGRAAADKSLDR
                     RAH"
     gene            complement(2020..2619)
                     /locus_tag="AC159P_05420"
     CDS             complement(2020..2619)
                     /locus_tag="AC159P_05420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694926.1"
                     /translation="MILARTLRTLSLLLVLGVVCVAQESLPSPEPEEFPTKLEELLEK
                     AGAVVVKGTTGVGSVSGLRGTAFVTSWEILDARSGRREYGISIEIRESTPPERERERE
                     RGDVAYVDYDELDPLINGLDHLLKLDNTATKLSTFEAQYRTNGYLSVFRFNTTGGYGT
                     AVSAGGRRGTRLILRPTGLVEFRDMLKSAKAVIDEARQK"
BASE COUNT          431 a          934 c          915 g          424 t
ORIGIN      
        1 cgcgcggcgg cacgtcgggg aagatgtccg ggttgtgcgt gagcaggagc acgggctctt
       61 cgtccggccc gactcccttc aggctcgcgg acacgtcggc ggtgcgcgtc cacatgtcgg
      121 ccacacccgc cacgtggaaa gcgcgcccgc cacgctcgac gcgcaccgac tggttttcga
      181 gaaccgtcac gcccgccgcc ttgagcgcgg tgcgaaccct cggcccatcg agccaccagt
      241 cgtgattgcc gagcaccgcg acgacgccgt gcggtgcctt caggtttttc aactcctccg
      301 cgatgcgctc gggcgtgacg aactcgccgc ccgccacacc ctgtatcacc agatcgccga
      361 gaatcacgac gagatcgggc tgctcctcgt tcgtcagttc gaccacgcgc cgcagcttct
      421 ccggcccgac gtgcggcgag ccgacgtgca gatccgtcaa cacggcgacc ttcatcccgc
      481 cgtgctccgc gcgccacgcc ggaaccttca ggctcacacg ccgcacgccg agcgaggccg
      541 gttcgagcca gaacgcccac agcgccagcg cgcacgcgac ggcgaacgcc gcgacggtga
      601 tttttccgag cttagacttg aaggctgttg ataaaggcat cgtgccgcga cgctcttcga
      661 aagttggcga atggccggcg gaaggctctg tgcttcacgc gcggcgccac gcggagagcg
      721 gcaagccctt gccgccgcgg cggtgggcga cgatctcggc gaggacggcg agggcgacct
      781 cttcgggcgt gaccgcgccg aggtcgaggc cgatgggcgc gcgcacctcg cgcaaggcgt
      841 cttccgcgaa gcccgactcg cgcagccgcg cgagcacgat gttcgtgcgc cgacggctgc
      901 cgatcatgcc gacgtagtcg ggccgcgccg cgagcactgc gcgcatgcac tcttcgtcct
      961 cgttgtggcc gcgcgtgacg accgcgacgg cgacgccgcg ccccgtgccg acggtctcgc
     1021 gcaccggccc ggtccaatcg gacgcggcga cgagcgcgat gccttcttcg gggaagcgcg
     1081 cgcgcgcgac gaactctgcg cggtcgtcga tgagcgtgac gcggtagccg atcgattgcg
     1141 ccagccgcgc gagggcggcg ccgacgtggc ccgcgccgca gacgacgacg tgcggctcgg
     1201 gcgcgacgcg ctcgaacaac acgcgcgcgc cgcgccacgc ttcgagcgcg ggcgcgaact
     1261 cctcgacgcg cagtgtgcgc gcctcggcgc gcgacgcgag gaacgcggcg gcgtgtcccg
     1321 cgatggcttc gtcgaacgcc gcgtcgccgg tcgtccccgc gcgcgcgcct ccgtcgtctt
     1381 cgaacaggat tttcgcgccg accgagtgcg gcgccgagat gaccgtggcg agggcggcga
     1441 cggcgccctc gccgagaacg cgcgcgagcg cgcgcgtgag cctttcgggg gtggatgagt
     1501 cttcgcccgc gcccatgcgg ctttcagtgc gcgcggcggt cgagcgactt gtcggcggcg
     1561 gcgcgcccga ggccgatggc cgcgagcgcg cggcgcacga gccacagagg gccgacgagc
     1621 aggtagatag ggttgccgaa gaaggcaggc cggttgccct cgatggcgtg gccgatgaac
     1681 tggaagaccc acccgacgac gaagagcgcg agcgcccacc gccagctaaa gaagaagagc
     1741 ggcaggctca cgacgatcat cgggatgccg atggcgtgcg tcaggtggtt cagagggtgc
     1801 tggtgcttcg ccttgtactc ttcgatgaac gatgtcggca tgtgaacctc caaaggccgt
     1861 caaccgtgaa ccgtcaaccg tgaatggtgt ttgagtggcg accgaacatg aatggtaaat
     1921 ctttacgccg cccgacgaca agcctccgac acaccaaccg acacttgcca ttcacgattt
     1981 acggtttccg gttcacggtt tccggttcac ggcctttcac tacttttgcc gcgcctcgtc
     2041 gatgacggct ttggcgctct tcagcatgtc gcggaattcg acgaggcccg tgggccgcag
     2101 gatgaggcgc gtgccgcgtc gcccgccggc ggagacggcg gtgccgtagc cgcccgtcgt
     2161 gttgaagcgg aagaccgaga ggtagccgtt ggttctgtac tgcgcctcga aggtggagag
     2221 cttcgtcgcc gtgttgtcga gcttgagcag gtggtcgagg ccgttgatca gcgggtcgag
     2281 ttcgtcgtag tccacatagg ccacgtcgcc ccgctcgcgc tcgcgttccc tctcgggcgg
     2341 cgtgctctcc ctgatctcga tgctgatgcc gtactccctg cgccccgacc gcgcgtcgag
     2401 aatctcccag ctcgtgacga aggccgtgcc cctcaagccc gacaccgagc cgacgcccgt
     2461 cgtccccttg acgaccaccg cccccgcttt ctcaagcagc tcctccaact tggtcgggaa
     2521 ctcttccggc tccggcgacg gcagcgactc ctgcgcgaca catacgacgc cgagcacgag
     2581 cagcagcgag agcgtgcgca aagtgcgtgc gagtatcacg ggcgctcctt tcaagaaaag
     2641 tactgaggag cggaggactg agtaaaggct ttgactgtcg atgagaggca cgctgttcct
     2701 actc
//
LOCUS       DBNGOH010000284         3619 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2792283, whole genome shotgun sequence.
ACCESSION   DBNGOH010000284 DBNGOH010000000
VERSION     DBNGOH010000284.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3619)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3619)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3619
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2792283"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            372..3494
                     /locus_tag="AC159P_05425"
     CDS             372..3494
                     /locus_tag="AC159P_05425"
                     /inference="COORDINATES: protein motif:HMM:NF012706.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR3694927.1"
                     /translation="MSTVINHNGADSLNAAGASPNGHAAKRKRPARKATKAAPAHAEM
                     ERVAALLADGDLAAEFSAEGAGAGERRALEELTALARSLRRAVGRLQRAADSVETVSK
                     RVLEEGRVLALAVGDEAVSVDATVASIAEISGSSRSVANSVSALAYLAQSTSTSALEM
                     AGSIDEVSSSADALTAFVEETATSIEEMATSVKGVAASTESLAQATDETERSMRAIDE
                     STQRVGLAVSETAVLAEEVRHSAEQGSAVVAETAESMRAMRGGIEEAGRTIAALGERS
                     ERIGAVTRVIDEIADRTNLLALNARILAAQAGQHGRGFAVVAEEIKELSERTARSTEE
                     IEELIKDVRESVAEAASQSASNRQLADEGVQLAERAAASLKEISDKTNLSAIAIRQIA
                     DAATVQALESHQVTEQTGHVRRRAQEIERATSAQARTAAEIGGRAVRMAELTGQVRRA
                     MREQTEASKHIAMAMEQLTEVVGQIAHAVGEQYRGAEEVLSAVEVIREAVTQNLASIV
                     QINAAAGLLDYEAASLRETVGHFKLPAPRRGGHLHFGVYAKNASLDALEASTVARAEL
                     SSLIFECLVGAGDGAEVEPRLAEEWDISADGRTYTFRLREGVRFHNGRLMTAEDVVYS
                     VRRTLRQSKSGAWVFKNLVGAEEFARGASEELAGVRRLDERSVALELDEPLAFFLPML
                     CLPFAAVVPREEVERDGGAVFLEGPVGTGPFKVVSCDRGAGRVELARTESYWDGDKPH
                     VESVSVEFSENGEHLFERMRAGELAFMREGSAQRVARLASDPEWRSCVVLAQQLHTQM
                     LVFDAEQPPFDDVRVRRAFAHAIDRRRLVEEAYGDMASPASGPVPPGLMGHDPDYRGL
                     EHDPELARRLLYVAGYRAGLKLTYWRTYSEQSASERAGCIVREQLAAVGVECDVRVAD
                     AAELIGSALAGRAPLAELSWYADYADPDNFTYVLFHSANRASSVGRTARVEEVDTLSA
                     RARTTVNHAERARLYTRLQHLIAEEALGAFLTHRRVAVIHRPDVEGLHAHLVSPVVRP
                     QEIWIRKSDG"
BASE COUNT          559 a         1292 c         1251 g          517 t
ORIGIN      
        1 gcaccgcctg ctcggcgtcc tcggcggcgc ggccccgcgc cacgcccgcg aaccgcgcgg
       61 cctcgccgtc cgcctcaacc gccccgcccg cccgacggaa gcgtaggcag tggggagaag
      121 gggcgaaagg gagtgggggg caaaggcaag caatgctttt ctcccccact ccctttctcc
      181 ccttctcccc cactgccttt attggtcttg aactttgcac cttgagcttc tatgatgaat
      241 gtggacggcg cgccccttcg aaggccgtcc acggttcctg atcccacctc tcgcgggcgg
      301 ccccgagccg cctcccgaca cagcaagccg cgcacgccct tcggtcgccc tccggtcttt
      361 cggataatcc tatgtctacg gtcatcaatc acaacggcgc ggactctttg aacgccgcgg
      421 gcgcgtcgcc gaacggccac gcggcgaagc ggaaaaggcc ggcgcgtaag gcgacgaaag
      481 ccgcccccgc gcacgccgag atggagcgtg tggcggcgct gttggcggac ggcgatctgg
      541 cggcggagtt ctcggcggaa ggcgcgggcg cgggcgagcg gcgcgcgctc gaagagttga
      601 cggcgctggc gcgcagcctg cggcgcgcgg tcgggcgctt gcagcgcgcg gcggattcgg
      661 tcgagaccgt ctcgaagcgc gtcctcgaag aggggcgcgt gctggcgctc gccgtcggcg
      721 acgaggccgt ctcggtcgat gccaccgtcg cttcgattgc cgaaatcagc ggctcctcgc
      781 gctcggtcgc gaactccgtg agcgcgctcg cctacctcgc gcaatcgacc tcgacctcgg
      841 cgttggagat ggccggctcg attgacgagg tctcttcgag cgccgacgcg ctgaccgcgt
      901 tcgtcgaaga gaccgccacc tcgatcgagg agatggccac gtccgtcaag ggcgtggctg
      961 cctcgaccga atcgctggcg caggcgaccg acgagaccga gcggtcgatg cgcgcgatcg
     1021 acgaatcgac gcagcgcgtc ggcctcgccg tctccgagac ggcggtgctc gccgaagagg
     1081 tgcgccactc cgccgagcaa ggctcggcgg tcgtcgccga gaccgccgag tcgatgcggg
     1141 ccatgcgcgg cggcatcgaa gaggcgggcc gcaccatcgc cgcgctcggc gagcgctccg
     1201 aacgcatcgg cgccgtcacg cgcgtcatcg acgagatcgc cgaccgcacc aacctgctcg
     1261 ccctgaacgc ccgcatcctc gccgcgcagg ccggtcaaca cggtcgcggc ttcgccgtcg
     1321 tcgccgaaga gatcaaggag ttgtccgagc gcaccgcgcg ctcgaccgaa gagatcgagg
     1381 agttgatcaa agacgtgcgc gagtctgtcg ccgaagccgc cagccagtcg gcgagcaacc
     1441 gccagctcgc ggacgagggc gtccagctcg ccgagcgcgc cgccgcctcg ctcaaggaga
     1501 tcagcgacaa gacgaacctg tcggcgatcg ccatccggca gatcgccgac gcggcgacgg
     1561 tgcaggcgct cgaatcgcat caggtgacgg agcagaccgg ccacgtccgc cggcgcgcgc
     1621 aggagatcga gcgcgcgact tccgctcagg cgcgcaccgc cgccgagatc ggcggtcgcg
     1681 ccgtccgcat ggccgaactc acagggcagg tgcgccgcgc gatgcgcgag cagaccgaag
     1741 cctcgaagca catcgcgatg gcgatggagc agttgaccga ggtggtcggg cagatcgcgc
     1801 acgcggtcgg cgagcagtac cgcggcgcgg aggaagtctt gagcgcggtc gaagtcatcc
     1861 gcgaggcggt gacgcagaac ctggcttcca tcgtgcagat caacgccgcc gcgggcctgc
     1921 tcgactacga agctgcgtcc ttgcgcgaga cggtgggcca tttcaagctg cccgcgccgc
     1981 gccgcggcgg ccaccttcac ttcggcgtct acgcgaagaa cgcttcgctc gacgcgctcg
     2041 aagcctcgac ggttgcgcgc gccgagctgt cgagcctgat cttcgaatgc ctcgtcggcg
     2101 cgggcgacgg cgccgaggtc gagccgcgtc tcgccgaaga gtgggatatc tccgccgacg
     2161 gtcgcaccta cacgttccgc ctgcgcgagg gcgtgcgctt ccacaacggg cggctgatga
     2221 cggcggagga cgtggtctac tcggtgcgcc gcacgctgcg tcaatcgaag tcgggcgcgt
     2281 gggtcttcaa gaacctcgtc ggcgcggagg agttcgcgcg cggcgcgtcg gaagaactcg
     2341 cgggcgtgcg ccgactggac gaacgctcgg tcgcgctcga actcgacgag ccgctcgcct
     2401 tcttcctgcc gatgctgtgc ctgccgttcg cggcggtcgt cccgcgcgaa gaggtcgagc
     2461 gcgacggcgg cgctgtcttc ctcgaaggcc ccgtcggcac cggccctttc aaggtcgtct
     2521 cctgcgaccg cggcgcgggc cgcgtcgaac tcgcccgcac ggaaagttac tgggacggcg
     2581 acaagccgca cgtcgagagc gtctcggtcg agttcagcga gaacggcgag cacctgttcg
     2641 agcggatgcg cgcgggcgag ctggcgttca tgcgcgaagg ctcggcgcag cgcgtggcgc
     2701 ggctcgcgtc cgacccggag tggcgctcgt gcgtcgtgct cgcgcagcag ctccacacgc
     2761 agatgctcgt cttcgacgcc gagcagccgc ccttcgacga cgtgcgcgtg cgccgcgcct
     2821 tcgcgcacgc catcgaccgc cgccgcctcg tcgaagaggc ttacggcgac atggcgagtc
     2881 ccgcgagcgg ccccgtcccg cccggcctga tggggcacga ccccgactac cgcggcctcg
     2941 aacacgaccc cgagcttgcg cgccgcctgc tctacgtggc gggctaccgc gccggtctca
     3001 agctcactta ctggcgcacc tactccgaac agtccgcgag cgagcgcgca gggtgcatcg
     3061 tccgcgaaca gctcgcggct gtcggcgtcg agtgcgacgt gcgcgtggcg gacgccgccg
     3121 aactcatcgg gtcggcgctc gcgggccgcg cgccgctcgc cgagctgagc tggtacgccg
     3181 actacgcaga cccggacaac ttcacctacg tcctcttcca ctcggccaac cgcgcctcct
     3241 cggtcgggcg cacggcgcgc gtcgaggagg tggacacgct gtcggcgcgc gcgcgcacca
     3301 ccgtcaacca cgccgagcgc gcgcggctct acacgcggct ccaacacctc atcgcggagg
     3361 aggcgctcgg cgccttcctc acgcaccgcc gcgtcgccgt catccaccga ccggacgttg
     3421 aaggcttgca cgcgcacctc gtctcgccgg tcgtccgccc gcaggaaatc tggatccgaa
     3481 aaagtgacgg gtgacaaccg gcgggtgacg agataaaatc cgtccatcgt caatcgtgaa
     3541 tcgtggttga tgggtcgtcc gacctgagtg gtgaacctca agtccgacgg ggacgaagtg
     3601 tcttcacggt cgcgggggg
//
LOCUS       DBNGOH010000285         3584 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4442043, whole genome shotgun sequence.
ACCESSION   DBNGOH010000285 DBNGOH010000000
VERSION     DBNGOH010000285.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3584)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3584)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3584
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4442043"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..471
                     /locus_tag="AC159P_05430"
     CDS             <1..471
                     /locus_tag="AC159P_05430"
                     /inference="COORDINATES: protein motif:HMM:NF024242.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR3694928.1"
                     /translation="DLADELSENAAGQEVVLAALVEQTLVHVLRRYGNVRRSDHLELS
                     RAGLVDRRVRRAVELMHAHLEHDLPLEEIAAAAYLSPFHFARLFKKLTGTTPHAYLAA
                     LRAARAQTLLAETDLSITEVGARVGHASPSHFAKAFRQATGISPSAYRKALAGK"
     gene            796..1992
                     /locus_tag="AC159P_05435"
     CDS             796..1992
                     /locus_tag="AC159P_05435"
                     /inference="COORDINATES: protein motif:HMM:NF051229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HIR3694929.1"
                     /translation="MRNFSLRLATAFLTFAVGVTASAALLKRAATTCELPVTPVVDKP
                     SPDERTLEMVFVLDTTGSMGGLLEGAKQKIWGIVNEVMQSPDKPAVRVGLVAYRDRGD
                     QYVTQVLPITHDLDKVYTTLMEYRADGGGDGPENVRRALADGVRQAGWSKASERVSQV
                     IFLVGDAPPHDDYQDEPDTLVTTQEAVSRGMIVNTIQCGEATDTRAVWQNIARRGEGR
                     YFAIAQDGGVSTIATPYDSRLTELGGKLGATYMAYGGGPGEAGRDFRVKAEAEQSRTE
                     STVAANAAPVAAADRAANKVMNRDAYVGDLLQSIENGTVRLDAVKDDDLPDALQKLPP
                     AERKKEVERRLAERQKIRDEITSLSKQRAAFIAAERKKLTGAKAGFDSAVADALKEQM
                     TRKGKK"
     gene            2205..3524
                     /locus_tag="AC159P_05440"
     CDS             2205..3524
                     /locus_tag="AC159P_05440"
                     /EC_number="5.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004512 - inositol-3-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006021 - inositol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HIR3694930.1"
                     /translation="MSVEKNVNIEPAKGKLGVLLVGLGAVSTTFIAGVEAVKQGLAAP
                     VGSLTQMATIRLGKRTDNRTPPIKEFVPLADLKDLVFGAWDIFPDSAYEAALTAGVLE
                     KGLLAQLKTPLQKIKPMKAVFDQQYVKRLNGTNVKEGKNKFELAEQLNAEIAEFKRKN
                     KCARLVMVWAASTEIFLKPHAVHKTLKSFEQAMRDNHKSIAPSMLYAYAAIKSGVPFA
                     NGAPNLTVDVPALTELAVKNGVAVCGKDFKTGQTLMKTILAPGLKSRMLGLHGWYSTN
                     ILGNRDGEVLDDPESFKTKEESKLSVLEYILQPEVYPELYKDFSHVVRINYYPPRGDN
                     KEGWDNIDIFGWLGYPMQIKIDFLCRDSILAAPIVLDLALFLDLAQRAGMKGIQEWLS
                     FYFKSPMTAPELYPEHDVFIQLMKLKNTLRHMRGEDLITHLGLEYYD"
BASE COUNT          705 a         1271 c         1074 g          534 t
ORIGIN      
        1 gacctcgccg acgagttgag cgaaaacgcc gccgggcagg aggtcgtcct cgccgcgctc
       61 gtcgagcaga ccctcgtcca cgtgctgcgc cgctacggca acgtccgccg ctccgaccac
      121 ctcgagcttt cgcgcgcggg cctcgttgac cgccgcgtcc gccgcgccgt cgagctgatg
      181 cacgcgcacc tcgaacacga cctgccgctc gaagagatcg ccgccgccgc gtacctctcg
      241 cccttccact tcgcgcgcct cttcaaaaaa ctcacgggca ccacgccgca cgcctacctc
      301 gccgcgctgc gcgccgcccg cgcgcaaacc ctgctcgccg agaccgacct ctccatcacc
      361 gaagtcggcg cccgtgtcgg ccacgccagc cccagccact tcgccaaagc cttccgccaa
      421 gccaccggca tctcgcccag cgcctaccgc aaagcgctcg ccggaaagta ggcggtaggc
      481 ggtaggcagc aggcagtaaa aagaggatgg aagtcgagag ccaggaaccg gagcagtgaa
      541 ggtgcttggg aggtaaagtg tcaagcatct ccctctgact gcttgacgga cgaagcggct
      601 cggcacttta cctcccaagc accttcactc gtccgcctta actgcctacc gcctaccgcc
      661 tacttttttt gcaagccgcg cccgcgtggc ccgttcactc cggcagaagc gttggccccg
      721 acgggtgctc gcaagcaggc gggcgggatt cgagaaggcg gcccgcgtca atttttcgga
      781 aaggattttg aggccatgcg gaatttctct ctcagactcg ccaccgcgtt tttgactttc
      841 gccgtcgggg tgacggccag cgccgcgctg ctgaaacgtg ccgcgacgac gtgcgaactg
      901 ccggtcacgc ccgtcgtcga taagccttcg cctgacgagc ggacgctgga gatggtcttc
      961 gtcctcgaca cgaccggctc gatgggcggg ctgctcgaag gcgcgaagca gaaaatctgg
     1021 ggcatcgtca acgaggtgat gcagtcgccc gacaaacccg cggtgcgcgt cggcctcgtc
     1081 gcctaccgcg accgcggcga ccagtacgtg acgcaggtgc tccccatcac ccacgacctc
     1141 gacaaggtct acacgacgct gatggagtac cgcgccgacg gcggcggcga cgggccggag
     1201 aacgtgcggc gcgcgctggc cgacggcgtt cggcaggccg gctggtcgaa ggcttcggag
     1261 cgcgtctcgc aggtcatctt cctcgtcggc gacgcgccgc cgcacgacga ctatcaggac
     1321 gagccggaca cgctcgtgac cacccaggag gccgtgagcc gcgggatgat cgtcaacacc
     1381 atccagtgcg gcgaggcgac ggacacgcgc gccgtctggc agaacatcgc gcggcgcggc
     1441 gagggccgct acttcgccat cgcgcaggac ggcggcgtga gcaccatcgc cacgccctac
     1501 gactcgcgcc tcacggaact cggcggcaag ctcggcgcga cctacatggc ctacggcggc
     1561 ggccccggcg aggcgggacg cgatttccgt gtcaaggccg aggccgagca gtcgcgcacc
     1621 gaatcgaccg tcgccgcgaa cgcggcgccc gtggccgccg ccgaccgcgc cgccaacaag
     1681 gtgatgaacc gcgacgccta cgtcggcgac ctgctccagt caatcgagaa cggcaccgtc
     1741 aggctcgacg ccgtcaagga cgacgacctg cccgacgcgc tgcaaaagct cccgcccgcc
     1801 gagcgcaaga aggaggtcga gcggcgcctc gccgagcggc agaagatacg cgacgagatc
     1861 acctcgctct ccaaacagcg cgccgccttc atcgccgccg agcgcaagaa gctgaccggc
     1921 gccaaagccg gcttcgactc cgccgtcgcc gacgcgctca aagagcagat gacgcgcaag
     1981 ggcaagaagt aactccgaag acagggggaa aaggtcgaaa ggggtaacgg cgaaaaggga
     2041 aaagcaagac aaagcaagtc gcttgtcttc ttcccttttc accttttccc cttttcccct
     2101 ttttcgtaat gagcaatccg gcgactgtat tgcgcaagat atgacgagac gcgggcggcg
     2161 gtgtccgcta tccttctcgg ataacttcga ggaggaacga atcattgtca gttgagaaga
     2221 acgtcaacat cgagccggcc aagggtaagc tcggggtgct gctcgtcggc ctcggcgccg
     2281 tgagcacgac cttcatcgcg ggcgtcgagg ccgtcaagca gggcctcgcc gcgccggtcg
     2341 gcagcctgac gcagatggcc accatccgcc tcggcaagcg taccgacaac cgcaccccgc
     2401 ctatcaagga gttcgtgccg ctcgccgacc tgaaagacct cgtcttcggc gcgtgggaca
     2461 tcttccccga ctcggcttac gaggccgcgt tgaccgcggg cgtgctcgaa aaggggctgc
     2521 tcgcgcagct caagacgccg ctccagaaga tcaagccgat gaaggccgtc ttcgaccagc
     2581 agtacgtcaa gcgcctcaac gggacgaacg tcaaggaggg caagaacaag ttcgagctgg
     2641 ccgagcagct caacgcggag atcgccgagt tcaagcgcaa gaacaagtgc gcgcgcctcg
     2701 tcatggtctg ggccgcctcg accgaaatct tcctcaagcc gcacgccgtc cacaagaccc
     2761 tcaagtcctt cgagcaagcg atgcgcgaca accacaaatc gatcgcgccc tcgatgctct
     2821 acgcctacgc cgcgatcaag tcgggcgtcc ccttcgccaa cggcgccccg aacctgaccg
     2881 ttgacgtgcc cgcgctcacc gagctggccg tcaagaacgg cgtcgccgtc tgcggcaagg
     2941 atttcaagac cggccagacc ctgatgaaga ccatcctcgc gccgggcctc aagtcgcgca
     3001 tgctgggact gcacggctgg tactcgacca acatcctcgg caaccgcgac ggcgaagttt
     3061 tggacgaccc cgagtcgttc aagacgaagg aagagtcgaa gctctccgtc ctcgaataca
     3121 tcctccagcc cgaggtctac cccgaactct acaaggactt ctcgcacgtc gtccgcatca
     3181 actactaccc gccacgcggc gacaacaaag agggctggga caacatcgac atcttcggct
     3241 ggctcggcta cccgatgcag atcaagatcg acttcctgtg ccgcgactcg atcctcgccg
     3301 cgcccatcgt cctcgacctc gcgctcttcc tcgacctcgc gcagcgcgcc gggatgaagg
     3361 gcatacagga gtggctctcc ttctacttca agtcgccgat gaccgcgccc gagctgtacc
     3421 ccgagcacga cgtgttcatc cagttgatga agctcaagaa cacgctccgc cacatgcgcg
     3481 gcgaagacct catcacgcac ctcggcctcg aatactacga ctgaggcccg ccgactcgaa
     3541 cggaagcctt ggggcgcgcg agacacgact cgcgcgcccc ttcg
//
LOCUS       DBNGOH010000286         2729 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_775638, whole genome shotgun sequence.
ACCESSION   DBNGOH010000286 DBNGOH010000000
VERSION     DBNGOH010000286.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2729)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2729)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2729
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_775638"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..350
                     /locus_tag="AC159P_05445"
     CDS             <1..350
                     /locus_tag="AC159P_05445"
                     /inference="COORDINATES: protein motif:HMM:NF013003.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HIR3694931.1"
                     /translation="YKLDRIGLDGAKLRASNLSRISLYRAKLENVDLRNADLSHADLR
                     EANLRGARLDGARLEWTKLDGADLQGASLRGADLSGANVVSVNWESIKHDKDTIFPFA
                     NFQLPTSNPKGTT"
     gene            385..1731
                     /locus_tag="AC159P_05450"
     CDS             385..1731
                     /locus_tag="AC159P_05450"
                     /inference="COORDINATES: protein motif:HMM:NF013998.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HIR3694932.1"
                     /translation="MSSKGQSNGGRRNAILGTLIVLCGVAAAVLTVAARRSGDWELVR
                     VGAVASLLMVVVIVLFVVPPLVRGARAEAAQLDLPVQFTTGGVVFIGIFVVVSLAAWN
                     TGNNLLFLVFSVLFSTLFVAWTAARVSLLDLAVSARFPDHIFAGEAAPVIVTVHNTKR
                     VLPSVSVVVEARSRADSQPRGRGLARFQRERFERRVLAYFMYVPHGARVEQRVEQSFE
                     RRGHVLVTGFEISTRFPFGFFRIRRRLRARDVDLVIYPKPKPLGDDLHLLPIDAGQMQ
                     GARRGAGHDLHSLRDYQPQDDMRHIDWKATARLRQLVVREFTEEEERRVHVVFDTRMK
                     AGDEHVGARFEQGVGHAASLVSHFTDERAELALTIGDEQGRYGIGREHLYGCLRRLAL
                     VQPAAAAHEERARVEFWQRIAPPPSANGDGYVILITAAAPGTIPANVWRKSHVIYI"
     gene            complement(1840..2712)
                     /locus_tag="AC159P_05455"
     CDS             complement(1840..2712)
                     /locus_tag="AC159P_05455"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HIR3694933.1"
                     /translation="MTTALDLLKLMRPRQWVKSGFVLIGVLFAHAWHDAGTVRSVLLA
                     AAAFSLVASGVYVFNDLMDRAQDAAHPRKRERALAAGRVATGAAVVLLIVLLAAGFAL
                     GYLSSPVVLYILLAYVAINLAYSLGLKHVVLLDVFLIAGGFMLRLLAGTLGVRIAPSQ
                     WLLLCGLMLSLFLGFAKRRAELYAAAGGAEGGRKVLDNYQPVLLDKLIVVTASGAIIT
                     YSLYTTSEKTVQTHRTEALIYTVPFVMYGIFRYIYLLHNQRAGTDPAQEIFRDPHILL
                     SIAGWLALTVWLIS"
BASE COUNT          469 a          883 c          934 g          443 t
ORIGIN      
        1 cctacaaact ggataggatt gggttggacg gggcaaaatt gagggcgagt aacttgtcga
       61 ggatttcact gtacagggcg aagctggaaa acgtggattt gaggaacgct gatctaagtc
      121 atgctgactt gagggaggcg aacctgagag gcgccaggct ggacggggca cgactggaat
      181 ggacaaagct ggacggcgcc gacctacagg gcgcaagcct gagaggtgcc gacttgagcg
      241 gggcgaatgt cgtttcggtc aactgggaaa gcatcaaaca cgataaagat actattttcc
      301 ccttcgccaa tttccaactc ccaacttcga acccgaaagg gactacttaa ccaccgcttt
      361 acggcttggg gaaattactc cttcgtgtct tccaaagggc agtcaaacgg cggtcggcgc
      421 aacgcgattc tggggacgct gatcgtgttg tgcggcgtgg cggcggcggt gctgacggtc
      481 gcggcgcggc ggagcggcga ttgggagttg gtgcgggtcg gggccgtggc gtcgctgttg
      541 atggtcgtgg tgatcgtgct gttcgtcgtg ccgccgctgg tgcgcggggc gcgggccgag
      601 gcggcgcagc tcgacctgcc cgtgcagttc acgaccgggg gcgtcgtctt catcggcatc
      661 ttcgtggtcg tctcgctggc ggcgtggaac acggggaaca acctgctgtt cctcgtcttc
      721 tcggtgctct tctcgacgct cttcgtggcc tggacggcgg cgcgcgtctc gctgctcgac
      781 ctcgcggtgt cggcgcgctt ccccgaccac atcttcgcgg gcgaggccgc gcccgtcatc
      841 gtcaccgtcc acaacacgaa gcgcgtgctg ccttcggtct cggtcgtcgt cgaggcgcgg
      901 agccgcgccg acagtcaacc gcgcgggcgc ggcctcgcgc gcttccagcg cgagcggttc
      961 gagcggcgcg tgctggccta cttcatgtac gtgccgcacg gcgcgcgcgt cgagcagcgg
     1021 gtcgagcagt cgttcgagcg gcgcggccac gtcctcgtca cgggcttcga gatttcgacg
     1081 cgcttcccct tcggcttctt ccgcatccgc cggcgcctgc gcgcgcgcga tgtcgatctc
     1141 gtcatctacc cgaagccgaa gccgctgggc gacgacctcc acctgctgcc catcgacgcc
     1201 gggcagatgc agggcgcgcg ccgcggcgcg ggccacgacc tccactccct gcgcgactac
     1261 cagccgcagg acgacatgcg ccacatcgac tggaaggcga cggcgcggct gcgtcagctc
     1321 gtcgtgcgcg agttcacaga ggaggaggag cggcgcgtcc acgtcgtctt cgacacgcgc
     1381 atgaaggcgg gcgacgagca cgtgggcgcc cgcttcgagc agggcgtcgg ccacgccgcc
     1441 tcgctcgtct cgcacttcac ggacgagcgc gccgaactcg cgctcaccat cggcgacgag
     1501 caggggcgct acggcatcgg gcgcgaacac ctctacggct gcctgcgccg cctcgccctc
     1561 gtccaacccg ccgccgccgc gcacgaagag cgcgcccgcg tcgagttctg gcagcgcatc
     1621 gcccctccgc cctcggccaa cggcgacggc tacgtcatcc tcatcaccgc cgccgccccc
     1681 ggcaccatcc ccgccaacgt ctggcgcaag tcgcacgtga tttacatata aagaaggggg
     1741 aaaagggtaa aaggggaaag ggcgaaaagg gaaaggagag cgcttgataa cacggctcgt
     1801 tcttcctctt cgcctcttcc ccttttaacc ttttcccctt cagctaatca gccagaccgt
     1861 caacgcgagc cagccggcga tggagaggag gatgtgcggg tcgcggaaga tttcctgcgc
     1921 ggggtccgtg ccggcgcgct ggttgtggag caggtagatg tagcggaaga tgccgtacat
     1981 gacgaagggc acggtgtaga tgagcgcctc ggtgcggtgc gtctggacgg tcttctcgct
     2041 cgtcgtgtag aggctgtaag tgatgatcgc gcccgaggcc gtgacgacga tcagcttgtc
     2101 gagcagcacg ggctggtagt tatccagaac tttccgcccg ccctccgcgc cgcccgccgc
     2161 ggcgtacaac tccgcgcgcc gcttggcgaa gccgaggaac agcgagagca tcaggccgca
     2221 cagcagcagc cactgcgaag gggcgatgcg gacgccgagc gtgcccgcca gcaggcggag
     2281 catgaagccg cccgcgatga ggaacacgtc gagcaggacg acgtgcttga ggccgagcga
     2341 gtaggcgagg ttgatcgcga cgtacgcgag caggatgtag aggacgaccg gcgaagacag
     2401 gtagccgagg gcgaacccgg cggcgagcag cacgatcaag agcacgaccg ccgcgcccgt
     2461 cgccaccctc cccgccgcca gtgcccgctc gcgcttgcgc gggtgcgcgg cgtcctgcgc
     2521 gcgatccatc aggtcgttga agacgtagac gccgctcgcc acgagcgaga aggcggcggc
     2581 ggcgagcagc acgctcctga cggtgcccgc gtcgtgccac gcatgggcga acagcacgcc
     2641 gatcaggacg aagccgctct tgacccactg ccggggccgc atcagcttta acaaatccag
     2701 tgcggttgtc atcgggtgtt acgggagcc
//
LOCUS       DBNGOH010000287        12392 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3342638, whole genome shotgun sequence.
ACCESSION   DBNGOH010000287 DBNGOH010000000
VERSION     DBNGOH010000287.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 12392)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12392)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12392
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3342638"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2157
                     /locus_tag="AC159P_05460"
     CDS             <1..2157
                     /locus_tag="AC159P_05460"
                     /inference="COORDINATES: protein motif:HMM:NF012311.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HIR3694934.1"
                     /translation="LGIMLSDLRALRAGAAQAAPANATREKRDKLSPDLRMKLRESQG
                     DEEVDVIIETNGVVLGDSPSTDLATADGVVPGNSYKTFNLHSAKVKSQKLEALAARGD
                     IAHVSLDREVKQLGHVSLTAGADAASAMGGATPYDGTGIGIVVLDSGIDPNHLTFKDG
                     SNASRIVYKQDFTGETRTDDPYGHGTHVAALAAGNAAISGGAYKGIAPNAKIINLRVL
                     NSQGRGTVSTLLSALEWVLANRHNAAYNIKVVNMSLGTAAIDSSENDPLCRAARALVD
                     AGIVVVAAAGNEGKDSLGNKLYGQIHSPGNDPSVITVGAANTFGTDARTDDGVASYSS
                     RGPTRSFWTDAHGVKHYDNFIKPDLVAPGNKLIAAQAPGNRLVAENPALDADVSGAPT
                     KEQMFLSGTSMAAPVVAGAAALLKQANPSLTPNLVKTILMYTAQPLAGFNQFEQGAGQ
                     LNLDGAMRLAKLIRTDLGSATSVGEPLLCSNCAAPAPSTNIAGHTFQWGQGVVLDHTF
                     ATGTSLVTQYQKVYGLGVLLADMTVETNGVLITDSVLVSQGITLSESLVTSDGTTLGA
                     GIYFSGTGVMLADGVMLADGGRVLGDGVMLADGVMIADVTMQAVQSMLGGDSTGFMRV
                     TLDGTPTTLVASPSALRGQLNLTWVDLANNETGFRVERSSDGVNFTEVATVGANTKQF
                     NNTGLAPNTRYYYRIYAYGTKGSSEYSNVASAVTRK"
     gene            2389..4869
                     /locus_tag="AC159P_05465"
     CDS             2389..4869
                     /locus_tag="AC159P_05465"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HIR3694935.1"
                     /translation="MRAQPIAAAFQWSIIAVGAAVLLAAGYHAARGPLDPRFLVLAPL
                     TIAVGSRITIRIPGNKGNISVSDTFVFLAILLFGLNLAVLLAAAEAFFSSRRFCKKRV
                     TILFNAAVMACSTLVTVWAVRLVAGGTAVALREGYTANYIVGICVMALVQYVVNSCAV
                     ALAYSLLKAEPFWPTWRKHFLWTSISYFAGASAAGLVARLIDGAGFYAVLAAAPIIVI
                     VYFTYTTYLKNVEASAAQAEQAERHVLALRESEARFRSVFDHAAGMGLADEDGRWIKV
                     NRSLCEMLGYTERDLLARSFQAITHPDDLDTVLAQTAKLLAGEVATFQTELRYTHRRG
                     HPVWVLLSVTLNRSAQGRSANLIFQLQDIGDRKRAEERLVHDAFHDVLTGLPNRALFM
                     DHLKLSVERAKRRGDRLFAVLFLDLDRFKLINDSLGHMVGDRLLVGIARRLETCLRPG
                     DTVARLGGDEFTVLLEDLAGAGEATDVAERLQERLSRPFSLDGHEVFTTVSIGIALST
                     TGYDRAEDVLRDADTAMYRAKTLGKARHEVFDKAMHARALNLLQLETDLRRAVERREF
                     HLHFQPIVSLKGGAITGFEALARWQHPERGAVMPDEFIPVAEETGLIAPIGKLVLGDA
                     CRQLAVWQERYPQYPPLQVSVNLSGKQFMKPDLIEHVREVLAETGIEPRSLKLEITES
                     IMMENIETAPLMLAELRALGVELAIDDFGTGYSSLSYLHRFPINTLKIDRSFVGRMDG
                     NNENAEIVRTIIMLARSLEMDVVAEGVETEDQCAQLKALKCDYGQGYVFSRPVDAAGA
                     ERLLSASDTYMVVSAPYDLPPIHVPHDM"
     gene            complement(5056..6105)
                     /locus_tag="AC159P_05470"
     CDS             complement(5056..6105)
                     /locus_tag="AC159P_05470"
                     /inference="COORDINATES: protein motif:HMM:NF019844.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HIR3694936.1"
                     /translation="MNPLRRPPSHIAVAGARLRAFAACGLLLAFYSPSRAGAGALPPP
                     RQEASAERITTATGARLRTAPQTSADEVSKLALGVVVRELERSAATERIGAVEDFWYR
                     VAAPGGAEGWVFGGLTAPFDARRRAETYLKLAGDRLKIEEPNYADAVALLGFVERVAP
                     EMKTRAAVAELELLRLLALHKALGAIPLERLNDSPYKELVKEREALAVYSDPAGQWFV
                     RADALWELEKKYRGLPVAERIAWEASRLPLPGECEGYLPCTLSYTVSTEGQYLRLYPR
                     GAHAGEALDGISSLLDAVLEDAQSNNPVYEVPAADRASFRKELGDLGIIITRAGHPKG
                     AVVLQKLNQLAARFR"
     gene            complement(6210..7112)
                     /locus_tag="AC159P_05475"
     CDS             complement(6210..7112)
                     /locus_tag="AC159P_05475"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3694937.1"
                     /translation="MTKFTIRTAVVFAALLSAPLLGGGALSAHAQQQPQQQPTPRAPK
                     KKLQASSNFAQYAGRDSSNRLIAGGATRNITDPMAAGEKSFTERNYPEAIKAYKLAIQ
                     KHDAQSQYTLALAYYNLGVVHEVAGQHREAIAAYRRATGSNPDLGVAARAYYNLGNVL
                     AAEKKYAEAVAAYRKVAELAPDQYVVYYNIGLAQAAAGEHTDAAASFKQALGLKPDFA
                     DAHYNLGLSYWAAEQYDDSFAAFKEAVRLKPDHAEAHYNLGLAHLAFEDAEALEAEIK
                     ELEKLKPELAAKLREINKAEASQQ"
     gene            complement(7253..9262)
                     /locus_tag="AC159P_05480"
     CDS             complement(7253..9262)
                     /locus_tag="AC159P_05480"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017076.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HIR3694938.1"
                     /translation="MAKGKKRNIVSHLLRRWFLLLAVLLSLAVWVSLALAMRPLAESD
                     GRLGKNPFGIVTSQENRALDLLFQLRDAMHPETRRRAPSEPITIIKIDEASIRQSNVR
                     LQRWPREYYARLIDRAREGGARVIGLDLYLSEEGGVSAEDKAADQRLIDSLSQTDKIV
                     IVEKTQGGGTDAIKPLEMFAEYAYVGFADLPHDSDNAVRSSLLSWVAPDGEVHFSFGS
                     TLAQLYTGEELKAESPTTMRLGNRVLPLRPDAGIQIDFRGRTPAFQSISAGEILFKEG
                     AAIPDELFRDRIVIIGAYNNDAPDLFLTPYYESMALPRAFDKNLPITPAQMPGAEIHA
                     NAAATILFGNSLVRPAIWKSMLMLLVPLGLVGVAVFRLRSLPAFLVVVLVAAASLAVA
                     AWAFSSHSTVLPLATAWIGVGLLAPLGFVLRYAHERAERDAKEAERAQIMDIFSRCVS
                     PEVAEELYSKGEVVLGGELRVVTLIFTDIRGFTTLTEAATSSEQVVEWLNEYFSRMHK
                     VISFYGGHINKYIGDGLMIVFGAPVNRGEKLEARAAVACGLGMLEEIERINEDWKDMG
                     RPHIAIGVGIHTGEATCGVVGAPGRLEYTLIGDTVNLAARLESTTKDAGVPMLVSSTT
                     AAQLGESYETSPLGNVTVKGKTESTSVYTVKKKSAPAPAPAAVTA"
     gene            9597..>12392
                     /locus_tag="AC159P_05485"
     CDS             9597..>12392
                     /locus_tag="AC159P_05485"
                     /inference="COORDINATES: protein motif:HMM:NF012618.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HIR3694939.1"
                     /translation="MQQRRESVCDSTARAHAAGVGLLLLALVALAALVPAGARVDSRA
                     EAAGAQGASARPELVLQTGHALRVDAVAFSPDGQLVASASADNTVRLWDVASARELRN
                     LAGHTNYVKAVAFSPDGRTVATGSTDGTVKLWEVSTGREVRSLGGNGAVSSVAFGADA
                     SVLAAGNMAGAIKVWDAAAGRELSSQATAHAQQVASLAFSADGRLLASGGKDNAVKIR
                     DAATLAAVKTLAGHAGLVKAVAFSADGKWLASASFDKTVKLWNVEQGTEARTLAGHTN
                     KAVAVAFVADGARLLSADATKTVKVWDAKDGRELRTVAGSATPDSGEAPSETIAFSRD
                     AAQMATSNGDKSVQVRDVSAGTPERLFQSFSSGVYTTAFSPDKRWFATGSKDRTLRIW
                     EVSTGRKVRTLTGHTGFVTHIAFSPDGQWLVSGSHGGEAKLWNVNTGREERTLKGHTE
                     SINSVGFSADNKLVVTGSGDSTVRLWDLETGGELRVFKGHTAEVNAVALSPDRKWLAS
                     GGADKTVRLWDAATRQQARALPDNDSEVFSVAFSPDGRRLAAGSKDGKLRLWSVSEDG
                     PPRALAGHAAGVRAVAFSPDGRLLASGAADASVKLWDVANGAETRTLRGHADSVNSVA
                     FSPDGRWLSTGGEDAATRIWESATGELQATLVTMTDTADWLVVTPDGLFDGSPPAWNQ
                     ILWRFAGDTRAVAPVEVFFNEYFYPDLLSDILAGKRPRAARDIARIDRRQPVVKMSLA
                     GATAATANAATASRTVSVKIDVTDAPAGAQDLRLFRNGSLVKVWRGDVLRGQTRATVE
                     TTVPVVAGENYLTAYAFNRANVKSRDAALDIRGAASLRRPGTTYVLAVGVNSYANPEY
                     NLKFAVADARDFADEVRRQLQRLDGVLVWHNPIEGGRSRRVLDAMLREVAAQGVFVST
                     HPDTILRLGTKDVLVDSR"
BASE COUNT         1970 a         4417 c         4170 g         1835 t
ORIGIN      
        1 ctcggcatca tgttgagcga cctgcgcgcg ctgcgcgccg gggccgcgca ggccgcgccg
       61 gccaacgcga cgagggagaa gagggacaag ctcagcccgg acctgcgcat gaagctcaga
      121 gagtcgcagg gcgacgaaga ggtcgatgtc attatcgaga cgaacggcgt cgtgctcggc
      181 gactcgccct cgacggattt ggcgaccgcg gacggcgtcg tgccgggcaa cagctacaag
      241 acgttcaacc tgcactccgc caaggtgaag tcgcagaagc tggaggcgct cgccgcgcgc
      301 ggcgacatcg cgcacgtctc gctcgaccgc gaggtgaagc aactcggcca cgtctcgctg
      361 acggcgggcg cggacgcggc ttcggcgatg ggcggcgcca cgccttacga cggcaccggc
      421 atcggcatcg tcgtgctcga ctcgggcatc gacccgaacc acctcacctt caaggacggc
      481 agcaacgcct cgcgcatcgt ctacaagcag gacttcacgg gcgagacgcg caccgacgac
      541 ccctacggcc acggcacgca cgtggcggcg ctcgccgccg gcaacgcggc catctcgggc
      601 ggcgcctaca agggcatcgc gcccaacgcg aagatcatca acctgcgcgt gctcaactcg
      661 cagggccggg gcacggtctc gacgctgctc tcggcgctcg aatgggtgct ggcgaaccgc
      721 cacaacgcgg cctacaacat caaggtcgtc aacatgagcc tcggcacggc ggccatcgac
      781 tcctccgaga acgacccgct ctgccgcgcc gcgcgcgcgc tcgtggacgc cgggatcgtc
      841 gtcgtcgccg ccgccggcaa cgagggcaag gacagcctcg gcaacaagct ctacgggcag
      901 attcactcgc ccggcaacga cccgtcggtc atcacggtcg gcgccgcgaa taccttcggc
      961 accgacgcgc gcacggacga cggcgtcgcc agctacagct cgcgcggccc gacccgcagc
     1021 ttctggacgg acgcgcacgg cgtcaagcac tacgacaact tcatcaagcc cgacctcgtc
     1081 gcgcccggca acaagctcat cgccgcgcag gcgcccggca acaggctcgt cgccgagaac
     1141 cccgcgctcg acgcggacgt gtccggcgcc ccgaccaaag agcagatgtt cctgagcggc
     1201 acctcgatgg cggcgcccgt cgtcgccggg gccgccgcgc tcctcaagca ggccaacccc
     1261 tcgctgacgc cgaacctcgt caagacgatc ctgatgtaca cggcgcagcc gctcgccggc
     1321 ttcaaccagt tcgagcaggg cgccggccag ctcaacctcg acggcgcgat gcgcctcgcc
     1381 aaactcatcc gcaccgacct cggctccgcg acgagcgtcg gcgagccgct gctgtgctcc
     1441 aactgcgcgg cgcccgcgcc ttcgacgaac atcgccggcc acaccttcca gtgggggcag
     1501 ggcgtcgtcc tcgaccacac cttcgccacg gggacgagcc tcgtcacgca gtaccagaag
     1561 gtctacggcc tcggcgtgct cctcgccgac atgacggtcg agacgaacgg ggtgctgatc
     1621 acggacagcg tgctggtctc gcagggcatc acgctcagcg agagccttgt gaccagtgac
     1681 gggacgacgc tcggcgccgg catctacttc tccggcacgg gcgtgatgct cgccgacggc
     1741 gtgatgctcg ccgacggcgg cagggtgctc ggcgacggcg tgatgctggc cgacggcgtg
     1801 atgatcgccg acgtgacgat gcaggccgtg cagtccatgc tcggcggcga cagcacgggc
     1861 ttcatgaggg tcacgctgga cggcacgccc acgacgctgg tcgcgtcgcc ctcggccctg
     1921 cgtgggcagc tcaacctgac gtgggtcgat ctcgccaaca acgagaccgg cttccgcgtc
     1981 gagcgctctt ccgacggcgt caacttcacc gaggtcgcca ccgtcggcgc gaacaccaag
     2041 cagttcaaca acaccggcct cgccccgaac acgcgctact actaccgcat ctacgcctac
     2101 ggcacgaagg ggtcatccga atactccaac gtcgccagcg ccgtcaccag aaagtaaagg
     2161 gccgccaccg cccgcgtacc acgcggggcg gcgcgagaga ccgcaagctc gcgccgcccc
     2221 cgccgccctc gccgccccac agcaatcccg acagtagctt tcgacctaca ggcccgcgcc
     2281 gcgcgcgcgg ggtcggcctc accgccggcg agagagtgtg aaagccggga accctcgctg
     2341 cgcccgcccc ggaacgaaga gattccgaaa ggactgccgc cgagagccgt gagagcgcaa
     2401 cccatcgccg ccgccttcca gtggagcatc atcgccgtgg gcgccgccgt gctgctcgcc
     2461 gccggctacc acgccgcccg aggcccgctc gacccgcgct tcctcgtcct cgccccgctc
     2521 accatcgccg tcggctcgcg catcacgatc aggataccgg gcaacaaggg caacatctcc
     2581 gtctccgaca ccttcgtctt cctcgccatc ctcctcttcg gcctcaacct cgccgtgctg
     2641 ctggcggcgg cggaagcctt cttctcgtcg cgccgcttct gcaaaaagcg ggtcacgatt
     2701 ctcttcaacg ccgcggtgat ggcctgctcg acgctggtca cggtctgggc cgtgcgcctc
     2761 gtcgcgggcg gcacggcggt cgcgctgcgc gagggctaca cggccaacta catcgtcggc
     2821 atctgcgtga tggcgctcgt gcagtacgtc gtcaactcgt gcgcggtggc gctggcctac
     2881 tcgctcctga aggcggagcc gttctggccg acgtggcgca agcacttcct ctggacttcg
     2941 atctcgtact tcgcgggcgc ctccgccgcg gggctggtcg cccggctcat cgacggcgcc
     3001 ggcttctacg ccgtgctcgc cgccgcgccg atcatcgtca tcgtctactt cacctacacg
     3061 acctacctca agaacgtcga ggcttccgcc gcgcaggccg agcaggccga gcgccacgtc
     3121 ctcgcgctgc gcgaaagtga ggcgcgcttc cgcagcgtct tcgaccacgc cgccgggatg
     3181 ggactcgccg acgaggacgg gcgctggatc aaggtcaacc gctctctgtg cgagatgctc
     3241 ggctacaccg agcgcgacct gctggcccgg agcttccaag ccatcacgca cccggacgac
     3301 ctcgacaccg tgctcgcgca gaccgcgaag ctgctggccg gagaggtcgc gaccttccag
     3361 accgagctgc gctacacgca ccgccgcggc caccccgtgt gggtgctctt gagcgtgacg
     3421 ctgaaccgca gcgcgcaggg gcgctcggcc aacctcatct tccagcttca ggacatcggc
     3481 gaccgcaagc gcgccgagga gcgtctcgtg cacgacgcct tccatgatgt gctgacgggg
     3541 ctgcccaacc gcgcgctctt catggatcac ctcaagctct cggtcgagcg cgccaagcgg
     3601 cgcggcgacc gcctcttcgc cgtcctcttc ctcgacctcg accgcttcaa gctcatcaac
     3661 gatagcttgg gccacatggt cggcgaccgc ctgctcgtcg gcatcgcgcg ccgcctcgaa
     3721 acctgcctcc ggcccggcga cacggtcgcg cgcctcggcg gcgacgagtt cacagtcctg
     3781 ctcgaagacc tcgcgggggc gggcgaggcg acggacgtgg ccgagcgcct ccaggaaaga
     3841 ctctcgcgcc ccttcagcct cgacggccac gaggtcttca ccaccgtcag catcggcatc
     3901 gcgctctcga ccaccggcta cgaccgcgcc gaggacgtgc tgcgcgacgc cgacaccgcc
     3961 atgtaccgcg ccaagacgct cggcaaggcc cgccacgagg tcttcgacaa ggcgatgcac
     4021 gcgcgcgcgc tgaacctgct gcaactcgaa accgacctgc gccgcgccgt cgagcgccgc
     4081 gagttccacc tccacttcca gcccatcgtc tctctgaagg gcggcgccat caccggcttc
     4141 gaggcgctcg cgcgctggca gcaccccgag cgcggcgccg tcatgcccga cgagttcatc
     4201 ccggtcgccg aggagaccgg cctcatcgcg cccatcggca agctcgttct cggcgacgcc
     4261 tgccgccagc tcgccgtctg gcaggagcgc tacccgcagt acccgccgct ccaggtgagc
     4321 gtcaacctct cgggcaagca attcatgaag cccgacctca tcgagcacgt ccgcgaggtg
     4381 ctggccgaga ccggcatcga gccgcgcagc ctcaagctcg aaatcaccga gagcatcatg
     4441 atggagaaca tcgagaccgc gccgctgatg ctcgccgagc tgcgcgcgct cggcgtcgag
     4501 ctggccatcg acgacttcgg caccggctac tcctcgctct cctacctcca ccgcttcccg
     4561 atcaatacgc tcaagatcga ccgctccttc gtcggtcgca tggacggcaa caacgagaac
     4621 gcggagatcg tccgcaccat catcatgctc gcgcgcagcc ttgagatgga cgtggtggcc
     4681 gagggcgtcg agaccgaaga ccagtgcgcg cagctcaagg cactcaagtg cgattacggg
     4741 cagggctacg tcttctcgcg cccggtggac gccgcgggcg ccgagcgcct gctctccgcg
     4801 agcgacacct acatggtcgt ctccgccccc tacgacctgc cgcccatcca cgtcccgcat
     4861 gacatgtagg cgagtaggca gcagacagca ggcagtaggc agtcaagaca gaaagagcga
     4921 agaagcttgg gaggtaaagt gcaaagcctc cccactcttc aagcgataaa gcaagaggct
     4981 ttgagccgtt gcgcctcaag cctcttcttt cttctggctt ctgacttctt ctgcctaccg
     5041 cctaccgcct acttcttagc gaaagcgggc ggccaactgg ttgagcttct gcaagaccac
     5101 ggcgcccttc gggtggccgg cgcgcgtgat gatgatgccg aggtcgccca gctccttgcg
     5161 gaaggaggcg cggtctgcgg cggggacttc gtagacgggg ttgttgctct gcgcgtcttc
     5221 gagcacggcg tcgagcagcg acgagatgcc gtcgagggct tcgcccgcgt gcgcgccgcg
     5281 cgggtagagc cggaggtact ggccctcggt cgagacggtg tagctcaggg tgcagggcag
     5341 gtagccttcg cactcgccgg ggagcgggag gcgcgaagcc tcccacgcga tgcgctcggc
     5401 gacgggcagg ccgcggtact tcttctccag ctcccacagc gcgtcggcgc gcacgaacca
     5461 ctggcccgcc gggtcgctgt agacggcgag cgcctcgcgc tccttcacca gctccttgta
     5521 aggcgagtcg ttcaacctct ccagagggat cgcgccgagc gccttgtgga gcgcgagcag
     5581 ccgcagcagc tcaagctccg cgaccgccgc gcgcgtcttc atctccggcg ccacgcgctc
     5641 gacgaacccg agcagggcga cggcgtcggc gtagttcggc tcttcgatct tcaggcggtc
     5701 gcccgcgagc ttcaggtacg tctccgcgcg gcgccgcgcg tcgaacggcg cggtcagccc
     5761 gccgaacacc cacccctcgg ccccgcccgg cgcggcgacg cgataccaga agtcctcgac
     5821 cgcgccgatt ctctccgtcg ccgccgaacg ctccagctct cgcacgacca cgccgagcgc
     5881 gagcttcgac acctcgtcgg cactcgtctg cggcgcggtg cgcaggcgcg cgcccgtcgc
     5941 cgtcgtgatg cgctccgccg acgcttcctg ccgcggcggc ggcaaggcgc ccgcgcccgc
     6001 gcgcgacggg ctgtagaacg cgagcagcag cccgcacgcg gcgaaggcgc ggagccgcgc
     6061 gccggcgacc gcgatatgcg aaggggggcg acgaagggga ttcatctcac tttacctctc
     6121 gcccgagagg cgggcttgtg gccgccgctt caagccccgc gcctgcttga agcggcggcc
     6181 acagcccgcc tcccggacgt gaacgcgttc tactgctgcg aggcttcggc cttgttgatc
     6241 tccctgagct tggccgccag ctcgggcttg agtttttcga gttccttgat ctccgcctca
     6301 agcgcctccg cgtcttcgaa cgcgaggtgc gcgaggccga ggttgtagtg cgcctcggcg
     6361 tggtcgggct tcaggcggac ggcctccttg aaggcggcga acgagtcgtc gtactgctcg
     6421 gcggcccagt aggagaggcc gaggttgtag tgcgcgtcgg cgaaatccgg cttgaggccg
     6481 agcgcctgct tgaaggacgc ggcggcgtcc gtgtgctcgc ccgccgcggc ctgcgccagc
     6541 ccgatgttgt agtagacgac gtactggtcg ggcgccagct cggcgacctt ccggtaggcg
     6601 gcgacggcct cggcgtactt cttctcggcg gcgaggacgt tgccgaggtt gtagtaggcg
     6661 cgggcggcga ccccgaggtc ggggttcgac ccggtcgcgc ggcggtaggc cgcaatcgcc
     6721 tccctgtgct gcccggcgac ctcgtggacg acgccgaggt tgtagtaggc cagcgcgagc
     6781 gtgtactgcg actgcgcgtc gtgcttctgg atggcgagct tgtacgcctt gatggcctcc
     6841 gggtagttcc gctccgtgaa gctcttctcg ccggcggcca tcgggtcggt aatgttgcgc
     6901 gtggcgccgc ccgcgatgag gcggttcgac gagtcgcgcc cggcgtactg cgcgaagttc
     6961 gagctggcct gaagcttctt cttcggcgcg cggggcgtcg gctgctgctg gggctgctgc
     7021 tgcgcgtgcg ccgagagggc gccgccgccg aggagcggcg ccgagagcag ggccgcgaag
     7081 acgacggcgg tgcggatggt gaacttggtc atgactgatt ccttctttcc ttgaacggac
     7141 aatggcttca actcctcgaa cgagagccga cagacgttac gtcggcgccg aatgattcgg
     7201 atgcggcggg cgggcgaaat ctttgcccgc ccgcgtcgcg ggaggcgacg cttcacgccg
     7261 tcacggcggc gggggccggc gcgggcgcgc tcttcttctt cacggtgtag accgaggtgc
     7321 tctcggtctt gcccttcacg gtgacgttgc cgagcgggct ggtctcgtag ctctcgccga
     7381 gctgcgcggc ggtggtgctg ctgacgagca tcgggacgcc cgcgtccttc gtcgtcgatt
     7441 cgaggcgggc ggcgaggttc accgtgtcgc cgatgagcgt gtattcgagc cgaccgggcg
     7501 cgccgacgac gccgcacgtc gcctcgcccg tgtggatgcc gacgccgatg gcgatgtgcg
     7561 ggcggcccat gtccttccag tcctcgttga tgcgctcgat ctcttcgagc atcccgaggc
     7621 cgcacgccac cgccgcgcgc gcctccagct tctcgccgcg gttgacgggc gcgccgaaga
     7681 cgatcatcag cccgtcgccg atgtacttgt tgatgtggcc gccgtagaag ctgatgacct
     7741 tgtgcatccg gctgaagtat tcgttcagcc actcgaccac ctgttcggac gacgtggcgg
     7801 cctcggtcag cgtcgtgaag ccgcggatgt cggtgaagat gagcgtgacg acgcgcagct
     7861 cgccacccaa aacgacctcg cccttgctgt acagctcttc ggccacctcg ggcgagacgc
     7921 agcgcgagaa gatgtccatg atctgcgcgc gctcggcctc cttggcgtcg cgctcggcgc
     7981 gctcgtgcgc gtagcgcagg acgaagccga gcggggcgag caagccgacc ccgatccagg
     8041 cggtcgccag cggcagcacg gtcgagtgcg agctgaaggc ccacgccgcg acggccagcg
     8101 aggccgccgc gacgagcacg acgacgagga acgccggcag cgagcgcagc cggaagacgg
     8161 ccacgccgac cagcccgagc ggcaccagca gcatcaacat gctcttccag atggcgggcc
     8221 ggacgaggct gttgccgaac aggatggtcg ccgccgcgtt ggcgtgaatc tcggcgccgg
     8281 gcatctgcgc gggcgtgatg ggcagatttt tgtcgaaggc gcgcggcagc gccatcgact
     8341 cgtagtaggg cgtcaggaac agatcgggcg cgtcgttgtt gtaagcgccg atgatgacga
     8401 tgcggtcgcg gaacagctcg tccggaatcg cggcgccttc tttgaagaga atctcgcccg
     8461 ccgagatgct ctggaaagcg ggcgtgcgcc cgcggaagtc gatctggatg ccggcgtcgg
     8521 gccggagcgg cagcacgcgg ttgccgagcc gcatcgtggt cggcgactcg gccttcagct
     8581 cctcgcccgt gtagagctgc gccagcgtcg agccgaagct gaaatgcacc tcgccgtcgg
     8641 gcgccaccca gctgaggagc gacgagcgca ccgcgttgtc gctgtcgtgc ggcaggtcgg
     8701 cgaagccgac gtacgcgtat tcggcgaaca tctccaaagg cttgatggcg tccgtgcccc
     8761 cgccctgggt cttctcgacg atgacgatct tgtcggtctg cgacagcgag tcgatcaggc
     8821 gctggtcggc ggccttgtcc tctgccgaga cgccgccctc ctcgctcagg tagaggtcga
     8881 ggccgatgac gcgggcgccg ccctcgcgcg cgcggtcgat gagccgcgcg tagtattcgc
     8941 gcggccaccg ctggaggcgg acgttcgact ggcggatgga cgcctcgtcg atcttgatga
     9001 tggtgatcgg ctcgctcggg gcgcgccggc gcgtctccgg gtgcatcgcg tcgcgcaact
     9061 ggaagagcag gtcgagcgcg cggttctcct gtgaggtgac gatgccgaac gggttcttgc
     9121 cgaggcgccc gtccgattcc gccagcgggc gcatcgccag cgcgaggctc acccacaccg
     9181 ccagcgagag cagcacggcc agcaacagaa accagcggcg caggaggtgc gagacgatgt
     9241 tgcgcttctt gccttttgcc ataacggggg ctgtcggtcg gatgatgtga tacgggaatt
     9301 ccaagccgaa ggcatcaagg gtgttcgacc gggcgacggc ggcccgtgga ttgtatcgcc
     9361 cgcccgccgc cgagcgcaat ggaaagacaa ggggaagagg ggaaagagag ggaaaagggg
     9421 aagaggggaa gaggatgaag aagaggcgaa ggttttgcat ttctcctttt cgcctcttcc
     9481 ccttttaacc ttttcccccc tgctcccttt tagtattatc ttcacgccaa cccgccgcgc
     9541 gtgccgccgt ccgagcagcc ccgcccgccg gttttcaagc ggaaggaaac tcaatcatgc
     9601 agcagcgacg agagagtgtt tgcgactcaa cagcgcgcgc ccacgccgcg ggcgtcggcc
     9661 tcctcttgct ggcgctggtc gcgctcgcgg cgctcgtgcc cgcgggcgcg cgcgtcgatt
     9721 cgcgggcgga ggcagcgggc gcgcaagggg cgtcggcgcg gcccgagctt gtcctgcaaa
     9781 cgggccacgc gctgcgcgtg gacgccgtcg ccttcagccc cgacggccag ctcgtcgcgt
     9841 cggcgagcgc cgacaacacg gtgcggctct gggacgttgc ctccgcgcgc gagctgcgca
     9901 acctcgcggg ccacacgaac tacgtgaagg ccgtcgcctt cagccccgac gggcggacgg
     9961 tcgccacggg ttcgaccgac ggcacggtca agctctggga ggtctcgacg gggcgcgaag
    10021 tgcggagcct cggcggcaac ggcgccgtgt cgagcgtcgc cttcggcgcg gacgcctccg
    10081 tgctcgcggc gggcaacatg gcgggcgcga tcaaggtgtg ggacgcggcg gcggggcgcg
    10141 agttgtcttc gcaggcgacg gcgcacgcgc agcaggtcgc ctcgctcgcg ttcagcgctg
    10201 acgggcggct gctcgcctcc ggcggcaagg acaacgcggt gaaaatccgg gacgccgcga
    10261 cgctcgccgc cgtgaagacg ctcgcgggcc acgcggggtt ggtgaaggcc gtcgccttca
    10321 gcgcggacgg caagtggctg gcctcggcga gcttcgacaa gacggtgaag ctgtggaacg
    10381 tcgagcaggg gacggaagcg cgcacgctcg cgggccacac taacaaggcg gtcgccgtcg
    10441 ccttcgtcgc ggacggcgcg cggctcctct ccgccgacgc gacgaagacc gtcaaggtct
    10501 gggacgcgaa ggacgggcgc gagctgcgca ccgtcgccgg ctccgcgacg ccggactccg
    10561 gcgaagcgcc ttccgagacc atcgccttca gccgcgacgc cgcgcagatg gcgaccagca
    10621 acggcgacaa atccgtgcag gtgcgcgacg taagcgcggg cacgcccgag cgcctgttcc
    10681 agagcttttc gagcggcgtc tacacgaccg ccttcagccc cgacaagcgc tggttcgcca
    10741 cgggcagcaa agaccgcacg ctgcgcatct gggaagtctc gaccggtaga aaggtgcgca
    10801 cgctgacggg ccacacgggc ttcgtgacgc acatcgcctt cagccccgac gggcagtggc
    10861 tcgtctcggg cagccacggc ggcgaggcca agctctggaa cgtcaacacg gggcgcgagg
    10921 agcgcacgct gaaagggcac accgagagca tcaacagcgt cggcttcagc gccgacaaca
    10981 aactcgtcgt tacggggagc ggcgactcca cggtgcgcct gtgggacttg gagacgggcg
    11041 gcgagctgcg cgtcttcaaa ggccacacgg cggaggtcaa cgcggtcgcg ctcagccccg
    11101 acaggaaatg gctcgcctcg ggcggcgccg acaagaccgt ccgcctgtgg gacgccgcga
    11161 cccgacaaca ggcgcgcgcc ctgcccgaca acgacagcga ggtcttctcc gtcgccttca
    11221 gcccggacgg gcgccggctc gcggcgggga gtaaagacgg caagctaagg ctctggagcg
    11281 tgagcgaaga cggcccgccg cgcgcgctcg cgggccacgc cgcgggcgtg cgcgccgtcg
    11341 ccttcagccc cgacggtcgc ctgctcgcgt cgggcgccgc ggacgcgagc gtgaagctct
    11401 gggacgtggc gaacggcgcc gagacgcgca cgctgcgcgg gcacgccgac agcgtcaaca
    11461 gcgtcgcctt cagccccgac gggcgctggc tctcgacggg cggcgaggac gccgcgacgc
    11521 gcatctggga gtctgcgacc ggcgagctac aggcgacgct cgtcaccatg acggacacgg
    11581 cggactggct cgtcgtcacg cccgacgggc tgttcgacgg ctcgccgccc gcctggaatc
    11641 aaatcctctg gcgcttcgcg ggcgacacgc gcgcggtcgc gcccgtcgag gtcttcttca
    11701 acgagtactt ctaccccgac ctgctctccg acatcctcgc cggcaaacgc ccgcgcgccg
    11761 cgcgcgacat cgcccggatc gaccgccgcc agcccgtcgt caaaatgtcg ctcgcgggcg
    11821 caaccgccgc gacggcgaac gccgcgaccg cttcacgcac cgtctccgtc aagatcgacg
    11881 tgacggacgc gcccgcgggg gcgcaagacc tgcgcctgtt ccgcaacggc tcgctcgtca
    11941 aggtctggcg cggcgacgtg ctcagggggc aaacccgcgc gaccgtcgag accacggtgc
    12001 ccgtcgtcgc gggcgagaac tatctgacgg cctacgcctt caaccgcgcc aacgtcaaga
    12061 gccgcgacgc cgcgctcgac atccgcggcg ccgcctctct gcgccgcccc ggcacgacct
    12121 acgtgctcgc cgtcggcgtc aactcttacg ccaacccgga gtacaacctc aaattcgccg
    12181 tcgccgacgc gcgggacttc gccgacgagg tgcgccgcca gctgcaacgg ctcgacggcg
    12241 tgctcgtgtg gcacaacccg atcgaaggcg gtcgatcgcg gcgcgtgctc gacgcgatgc
    12301 tgcgagaggt cgcggcgcag ggcgtcttcg tcagcacgca tcccgacacc atcctgcgac
    12361 tcggaaccaa ggacgtgctg gtcgacagcc gc
//
LOCUS       DBNGOH010000288         5482 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2059435, whole genome shotgun sequence.
ACCESSION   DBNGOH010000288 DBNGOH010000000
VERSION     DBNGOH010000288.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5482)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5482)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5482
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2059435"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            335..1318
                     /locus_tag="AC159P_05490"
     CDS             335..1318
                     /locus_tag="AC159P_05490"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HIR3694940.1"
                     /translation="MADLESVTGSNGGETVRCRYCGQLNQTRGDSGQAKCGRCRLPLS
                     GAPHKKFADLDKHDYVHPADSRALAALRAIPGLDRAVKKLLQVLGEPQIRTILMASAV
                     RVTPKQAPDLHAKLQVACTTLGVDMPELYIVQSPIANAFTYGVERHVVVLFTGLIERL
                     SDDEILAVIAHEVGHIHAQHVLYLTVANLLELLAESAIKAAPIANLVVQILTTTMRAA
                     LLAWARRAELSCDRAALLVTQDPHVVGRTMMKLCGGTFASKLDYEEFLNQARDFQKNY
                     DEKALDRFWADIITSSLSHPFPIWRVSEILKWVESGEYQRLMEGTAESAAA"
     gene            1358..2122
                     /locus_tag="AC159P_05495"
     CDS             1358..2122
                     /locus_tag="AC159P_05495"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0030497 - fatty acid elongation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HIR3694941.1"
                     /translation="MMLLENKFGIIFGVANKRSIAWATAQACAEAGARLAFTYQNERL
                     KGNVEDLTGGMDAPLIQCDVTNPAEVAQTFAEVKNKFGRLDFLVHSLAYAPREALEGE
                     FVATTREAFLTALDISAYSLAELSRAALPLMTDGGSIISMTYYGAEKVVPNYNVMGVA
                     KAALEASTRYLASDLGKHNVRVNAISAGPIQTLAARGVGNLSTMMKHHAEHAPLRRNV
                     EPREVGNAALFLVSPLSTGITGEVLHVDCGYNIMAF"
     gene            2206..2952
                     /locus_tag="AC159P_05500"
     CDS             2206..2952
                     /locus_tag="AC159P_05500"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HIR3694942.1"
                     /translation="MADTKGKKRKGEGRQATQDEQLLESPRQDEFTHTDTWRVFRIMG
                     EFVEGFDELATVTRGVSIFGSARTPAEDPYYAAAQETAALLSKAGFAVITGGGPGIME
                     AANRGAREAGGVSIGCNIELPFEQAPNPYQTRSLTFRYFFVRKTMFIKYSTAFVIFPG
                     GFGTLDETFEALTLIQTRKIRNFPVVLYGAAYWGGLVKWIEETMMGARYINDTDLKLL
                     HLTDSPAEIVEIVVNSQDSLNKIDDIVYDY"
     gene            complement(2946..3338)
                     /gene="cutA"
                     /locus_tag="AC159P_05505"
     CDS             complement(2946..3338)
                     /gene="cutA"
                     /locus_tag="AC159P_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013130646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HIR3694943.1"
                     /translation="MSETQSAAIVVLMTAGSREEASRLAEMLVGAHLAACVQILPEME
                     SVYRWKGAVHREPEILLLAKTTAACFDELEREVRALHTYDKPEIVAVPVTHVSIPYFE
                     WITSNAFPPLVSRQAHEGAAASAAEEGQ"
     gene            complement(3444..3827)
                     /locus_tag="AC159P_05510"
     CDS             complement(3444..3827)
                     /locus_tag="AC159P_05510"
                     /inference="COORDINATES: protein motif:HMM:NF020967.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative signal transducing protein"
                     /protein_id="HIR3694944.1"
                     /translation="MFCPVCESEYEEGITRCPDDDVELVERLTPENTVHDNSEARFVP
                     LHNIGSPAEAEMVHDILTQNGVRSAVQSGGMDAFSPLLSTTAPGALVLVDERDLDRAQ
                     EIYASFFGGDTTPLTGATDEEENEG"
     gene            complement(4031..4972)
                     /locus_tag="AC159P_05515"
     CDS             complement(4031..4972)
                     /locus_tag="AC159P_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012471625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HIR3694945.1"
                     /translation="MSLLVVGSVAFDAVETPFGKREKMLGGSATHFSLSASFFADVRV
                     VGVVGGDFGAEELSVFERRGIDTGDLERVADGETFFWRGRYDFDLNTAHTLDTQLNVF
                     ADFQPKLSEASRGARMVFLGNIQPDLQREVREQVRGAELVALDTMNYWITSVPDAVKE
                     AISAVDVVIINDAEARQLSGEPNLIRAARAILSWGPRALVVKRGEYGAAMFTEGAYFA
                     IPAYPLESVFDPTGAGDTFAGGFMGYLASQDVADEAAMRRAIIFGSVMASFNVEEFGT
                     ERVRRLTHEEINERFAAFKRMTHFEEIPFERAVQATK"
     gene            complement(5098..>5482)
                     /locus_tag="AC159P_05520"
     CDS             complement(5098..>5482)
                     /locus_tag="AC159P_05520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HIR3694946.1"
                     /translation="DASVTRDPDVEKAAMKNLEAARHYFKLRKAYYASRERAEEILVA
                     FPDFTRTDEVLYIAGMSGLYLSEGKGKQAVPKTLPPDRAEEYTPERLRVSARFNLSRL
                     VENHPDSKFRKDAEAALKLLGPEKQ"
BASE COUNT          992 a         1807 c         1750 g          933 t
ORIGIN      
        1 aagcacggca tcaccgccgt catccagccc ggcggctccg tccgcgacgc cgaagtcgtc
       61 gccgccgccg acgagcacgg cctcgcgatg gtcttcacgg gcgtgcgaca ctttagacac
      121 taacagaggg tgttaggtgt taggtgttgg gtgttgggtg ctggtgaaaa gcaacttgcc
      181 ttgtctttca tccaataccc aacacctaac acccaaaacc ctttcttccc tgcttgccct
      241 cgcgccgcgc aatcgttgat acttgcagca ccgaacagct ttccagcacg tcttcccgga
      301 gagccttaat ttttttcacc gaagggaaag agagatggcc gatttggaat ccgtgaccgg
      361 ctcgaacggc ggcgagaccg tacgctgccg ctactgcggg cagctcaacc agacgcgcgg
      421 cgacagcggg caggccaagt gcgggcggtg ccgcctgccg ctgtcgggcg cgccgcacaa
      481 gaagttcgcc gacctcgaca agcacgacta cgtccacccg gcggacagcc gcgcgctcgc
      541 cgcgctgcgg gccatccccg gcctcgaccg cgccgtcaag aagctgctcc aggttctggg
      601 cgagccgcag atcaggacga tcctgatggc gagcgccgtg cgcgtcacgc cgaagcaggc
      661 gcccgatctg cacgcgaaat tgcaggtcgc ctgcacgacg ctcggcgtgg acatgcccga
      721 gctgtacatc gtccagtcgc ccatcgccaa cgccttcacc tacggcgtcg agcgccacgt
      781 cgtcgtcctc ttcacgggcc tcatcgagcg cctgtccgac gacgagatac tggccgtcat
      841 cgcgcacgag gtcggccaca tccacgcgca gcacgtcctc tatctgacgg tggcgaacct
      901 gctcgaactg cttgccgagt cggccatcaa ggcggcgccc atcgccaacc tcgtcgtgca
      961 aatcctgacc acgacgatgc gcgcggcgct gctggcgtgg gcgcggcggg ccgagctgtc
     1021 gtgcgaccgc gcggcgctgc tcgtcacgca ggacccgcac gtcgtcgggc gcacgatgat
     1081 gaagctgtgc ggcggcacct tcgcctcgaa gctcgactac gaagagttcc tcaaccaggc
     1141 gcgtgacttc cagaagaact acgacgagaa ggcgctcgac cgcttctggg ccgacatcat
     1201 cacgtccagc ctctcgcacc ccttccccat ctggcgcgtc tcggagattc tgaagtgggt
     1261 cgagtcgggc gagtatcagc gcctcatgga gggcaccgcc gagagcgcgg cggcgtgaag
     1321 cggtaagcag tattaacagt tgcggagaga gacaacgatc atgctgcttg agaacaagtt
     1381 cgggatcatc ttcggcgtcg ccaacaagcg ctcgattgcg tgggcgacgg cgcaggcgtg
     1441 cgccgaggcg ggcgcgcggc tcgccttcac ctaccagaac gagcgcctga aggggaacgt
     1501 cgaggatttg acgggcggca tggacgcgcc gctcatccag tgcgacgtga ccaacccggc
     1561 ggaggtcgcg cagaccttcg ccgaggtgaa gaacaagttc gggcggctcg acttcctcgt
     1621 ccacagcctc gcctacgccc cgcgcgaggc gctcgaaggc gagttcgtcg ccaccacgcg
     1681 cgaggcgttc ctcaccgcgc tcgacatcag cgcctactcg ctggccgagc tgtcgcgcgc
     1741 cgcgctcccg ctgatgaccg acggcggcag catcatctcg atgacctact acggcgccga
     1801 gaaggtcgtg cccaactaca acgtgatggg cgtggcgaag gccgcgctcg aagcgagcac
     1861 gcgctacctg gccagcgacc tcggcaagca caacgtccgc gtcaacgcca tcagcgccgg
     1921 ccccatccag acgctcgccg cgcgcggcgt cggcaacctc tccacgatga tgaagcacca
     1981 cgccgagcac gcccccttgc gccgcaacgt cgaacctcgc gaggtcggca acgccgctct
     2041 cttcctcgtc agccccctct ccaccggcat caccggcgaa gtcctccacg tggactgcgg
     2101 ctacaacatc atggctttct aaagtaggca gcaggcagca ggcagtaggc agttaagaaa
     2161 actgctttga ctgcctactg cctgctgcct actgcctact ttcttatggc tgacaccaaa
     2221 ggcaagaagc gcaagggcga ggggcggcag gcgacgcagg acgagcagtt gcttgagtcg
     2281 ccgcggcagg acgagttcac gcacacggac acgtggcgcg tgttccgcat catgggcgag
     2341 ttcgtcgagg ggttcgacga gctggcgacg gtgacgcggg gcgtctcgat cttcgggtcg
     2401 gcgcgcacgc ccgccgaaga cccgtactac gcggcggcgc aggagacggc ggcgctgctg
     2461 tcgaaggcgg gcttcgccgt catcacgggc ggcggccccg gcatcatgga ggcggccaac
     2521 cgcggcgcgc gcgaggcggg cggcgtctcc atcggctgca acatcgagct gcccttcgag
     2581 caggcgccga acccctacca gacgcgctcg ctgaccttca gatacttctt cgtccgcaag
     2641 acgatgttca tcaagtacag caccgccttc gtcatcttcc ccggcggctt cggcacgctc
     2701 gacgagacgt tcgaggccct cacgctcatc cagacgcgca agatcaggaa cttcccggtc
     2761 gtgctctacg gggcggccta ctggggcggg ctggtgaaat ggatcgagga gacgatgatg
     2821 ggggcgcgct acatcaacga cacggacctg aagctgctgc acctgacgga ctcgcccgcc
     2881 gagatcgtcg agatcgtcgt caacagtcag gactctctga acaagatcga cgacatcgtc
     2941 tacgactact gaccctcttc cgccgccgag gccgccgcgc cctcgtgcgc ttgccgcgag
     3001 acgagcggcg ggaatgcgtt cgaggtgatc cactcgaagt aagggatgga gacgtgcgtc
     3061 accggcacgg cgacgatctc cggcttgtcg taggtgtgca gagcgcgcac ctcgcgctcc
     3121 agctcgtcga agcaggcggc ggtcgtcttc gccagcagca ggatttccgg ctcgcggtgc
     3181 accgcgccct tccagcggta gaccgactcc atctccggca gtatctgcac gcacgccgcc
     3241 aggtgcgcgc ccaccagcat ctcggccagc cgcgaagcct cttcccggct ccccgccgtc
     3301 atcaacacca cgatggccgc cgactgcgtt tcactcatca cgaaccccct caagaaatga
     3361 tgaatgcgga ttgatgaatg atgaatcgaa gacaagtcga atgatgaaag gcgtcgtgct
     3421 tttcattcat cattccgcac tcatcatccc tcattctctt cttcgtcggt ggcgcccgtg
     3481 aggggcgtcg tgtcgccgcc gaagaaggag gcgtagattt cctgcgcgcg gtcgaggtcg
     3541 cgctcatcga cgaggacgag ggcgccgggc gcggtcgtcg agaggagcgg cgagaaggcg
     3601 tccatgccgc ccgactggac ggcggagcgg acgccgttct gcgtgaggat gtcgtgcacc
     3661 atctcggcct cggcgggcga gccgatgttg tgcagcggga cgaagcgcgc ctcgctgttg
     3721 tcgtgcacgg tgttctccgg cgtcagccgc tcgaccagct ccacgtcgtc gtccgggcag
     3781 cgcgtgatgc cctcctcata ctccgactcg caaacaggac agaacataca tccccctcga
     3841 agcgcctgat ttgtttggaa cgtgctgcat tatagggtga aaagacgaaa aggggaaaag
     3901 gcgagaagaa agagagggga aaaaggtgaa aaggggaaga ggggaaaagg ggaagaagga
     3961 ggatgagagg cacgctgcgt cttatcttcc cttttcccct cttccccttt tcaccttttc
     4021 gcccctgctt ctatttcgtc gcttgcacgg cgcgctcgaa gggtatctct tcgaagtggg
     4081 tcatgcgttt gaaggcggcg aagcgttcgt tgatctcttc gtgggtgagg cgccggacgc
     4141 gctcggtgcc gaactcttcg acgttgaagg aggccatcac ggagccgaag atgatggcgc
     4201 gtcgcatggc tgcttcgtcc gccacgtcct ggctggcgag gtagcccatg aagccgcccg
     4261 cgaaggtgtc gcccgcgccc gtcgggtcga agaccgattc gagcgggtag gccgggatgg
     4321 cgaagtaggc gccttcggtg aacatcgccg cgccgtactc gccgcgcttg acgacgagcg
     4381 cacgcggccc ccacgacagg atcgcgcgcg ccgcgcggat caggttcggc tcgcccgaga
     4441 gctgccgcgc ctcggcgtcg ttgatgatga ccacgtccac ggcgctgatc gcctccttca
     4501 ccgcgtcggg cacgctcgtg atccagtagt tcatcgtgtc gagcgcgacc agctcggcgc
     4561 cgcgcacctg ctcgcgcacc tcgcgctgga ggtcgggctg gatgttgccg aggaagacca
     4621 tccgcgcgcc gcgcgacgct tcggagagct tgggctggaa gtcggcgaag acgttgagct
     4681 gcgtgtcgag cgtgtgcgcc gtgttgaggt cgaaatcgta gcgcccgcgc cagaagaagg
     4741 tttcgccgtc ggcgaccctc tcaagatcgc ccgtgtcgat gccgcggcgc tcgaagaccg
     4801 acagctcctc cgcgccgaag tcgccgccga ctacgccgac cacccgcacg tccgcgaaga
     4861 aactcgccga caaagaaaaa tgggtggccg agccgcccag cattttttcg cgcttcccga
     4921 aaggcgtctc gaccgcgtcg aacgccaccg aaccgacgac taacaatgac actggtgggt
     4981 tttctccttt tagaaagtag gcagtaggcg gtaggcggta aaaacccttg tccagcggag
     5041 gagaaggtaa gagcgtcaac gtcgtccttg ctcgactaac cgcctaccgc ctactgttta
     5101 ctgcttctcc gggccgagga gcttgagcgc ggcctcggcg tccttgcgga acttgctgtc
     5161 ggggtggttc tcgacgaggc gcgagaggtt gaagcgcgcc gacacgcgga ggcgttcggg
     5221 cgtgtactct tcggcgcggt cgggcggcag cgtcttcggc acggcctgct tgcccttgcc
     5281 ctcggagaga taaagaccgc tcatccccgc gatgtagagc acctcgtcgg tgcgcgtgaa
     5341 gtcggggaag gcgacgagaa tctcttcggc gcgctcgcgc gaggcgtagt acgccttcct
     5401 cagcttgaag tagtggcgcg cggcctcaag gttcttcatc gccgccttct ccacgtccgg
     5461 gtcgcgcgtg acggacgcgt cc
//
LOCUS       DBNGOH010000289         2598 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_776515, whole genome shotgun sequence.
ACCESSION   DBNGOH010000289 DBNGOH010000000
VERSION     DBNGOH010000289.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2598)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2598)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2598
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_776515"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..443)
                     /locus_tag="AC159P_05525"
     CDS             complement(<1..443)
                     /locus_tag="AC159P_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017716718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HIR3694947.1"
                     /translation="MSDITLKDTGAIGEHPLDTEEALRRSSKELADLKFALDESAIVA
                     ITDQRGTINYVNDKFCEISKYSREELLGQDHRIINSAYHPKEFIRDIWTTIAGGDVWR
                     GEIRNRAKDGSLYWVDTTIVPFLDARGKPYQYVAIRYDITERKRA"
     gene            complement(459..1874)
                     /locus_tag="AC159P_05530"
     CDS             complement(459..1874)
                     /locus_tag="AC159P_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HIR3694948.1"
                     /translation="MLVVDDEQMIRWSFAQTLRGWGYVPIEAGTVADAISAYEHERPA
                     AVLLDIELPDGSGLDVLREIKGRHPEAVVIMVTANVLVENTIAALRGGAYDFVAKPPN
                     LDELRVRINNGIEAHQLRREIGQMRRERAREFGFDQIIGESPAMRRMLQTARKVAESE
                     ASSVLLQGASGTGKDLVAKAIHYGSRRSDAPFVAINCAAIPSTLIESELFGYEKGAFT
                     DAKARKEGLFEQAEGGTLFLDEIGELELGLQAKLLRVLEEGAFRRVGGLKDIPLDVRV
                     VAASNRDLKTEGEAGRFRIDLYYRLSVIQIDIPPLRERGDDTLLLADHYVRLFGERLR
                     LRKKMRGLSPVVREILLHSAWPGNVRELRNVIERALILEDGDLVTTEYLPRDLTGRVE
                     PASSSATVGAAGHPFTLPPEGVSLEEVESSLVRQAVERSGGNQTRAAELLGISRDQLR
                     YRLKKLESPAAEESAATESAP"
     gene            complement(2047..>2598)
                     /locus_tag="AC159P_05535"
     CDS             complement(2047..>2598)
                     /locus_tag="AC159P_05535"
                     /inference="COORDINATES: protein motif:HMM:NF014090.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HIR3694949.1"
                     /translation="GFANLAADGFSMAASNFLGTRAEREDFERLESVERRHIEVVPDG
                     EREEVRQIYRGKGFEGRELERVVALITSDRSRWVRTMLTEEYGLPGDIRSPWRAALAT
                     FSAFVLCGLVPLVPFLFGGRDALRLSILLTGGVFFAIGSAKSKWSTASWWRSGLSTLS
                     VGGLAASLAYVVGMLLARLASTP"
BASE COUNT          418 a          863 c          873 g          444 t
ORIGIN      
        1 tcggcgcgct tgcgttcggt gatgtcgtag cggatggcga cgtactggta gggcttgccc
       61 cgcgcgtcga ggaacgggac gatggtcgtg tccacccagt agagcgagcc gtccttcgcc
      121 cggttgcgga tctcgccgcg ccacacgtcg ccgcccgcga tggtcgtcca gatgtctcgg
      181 atgaactcct tcgggtggta ggccgagttg atgatgcggt gatcctgtcc gagcagctcc
      241 tcgcgcgagt acttggagat ttcgcagaac ttgtcgttga cgtagttgat cgtgccgcgc
      301 tggtcggtga tggcgacgat ggccgattcg tccaacgcga acttgaggtc ggccagctcc
      361 ttcgacgagc ggcgcagcgc ctcctcggtg tcgaggggat gctctccgat agctcccgta
      421 tctttcagcg tgatgtcgct catctcgggt cgaagccttc agggggcgga ctccgtcgca
      481 gccgactctt cggcagccgg gctttcgagc ttcttgaggc ggtagcgcaa ctgatcgcgc
      541 gagatgccga gcagctcggc ggcgcgcgtc tggttgccgc cgctgcgctc gacggcctgc
      601 cgcacgagcg aactctccac ctcttcgagc gagacgcctt cgggcgggag cgtgaagggg
      661 tgccccgccg cgcccacagt cgcggacgag gacgcaggct cgacgcgccc cgtcaggtcg
      721 cgcggcaggt actcggtcgt gacgaggtcg ccgtcttcga ggatgagcgc gcgctcgatg
      781 acgttgcgca gctcgcgcac gtttcccggc cacgcggaat gaagcaatat ctcgcgcacc
      841 acgggcgaga ggccgcgcat cttcttcctg agccgcagcc gctcgccgaa gagtcggacg
      901 tagtggtcgg ccagcaggag cgtgtcgtcg ccgcgctcgc gcaagggcgg gatgtcgatc
      961 tggatgaccg acagccggta gtagagatcg atgcggaagc gtcccgcttc gccctccgtc
     1021 ttgaggtcgc ggttcgaggc cgcgaccacg cgcacgtcga gcgggatgtc cttcagcccg
     1081 ccgacgcggc ggaacgcgcc ctcttccaaa acgcgcagca gcttcgcctg caaacccaac
     1141 tcgagttcgc cgatctcgtc gaggaagagc gtcccgccct cggcctgctc gaacaacccc
     1201 tccttgcgcg ccttcgcgtc ggtgaaggcg cccttctcgt aaccgaacag ctcggattcg
     1261 atgagcgtcg aagggatggc ggcgcagttg atggcgacga agggcgcgtc cgaacggcgc
     1321 gagccgtagt ggatggcctt cgccacgagg tctttgccgg tgcccgaagc gccctgcaag
     1381 agcacggacg aggcttccga ctcggccacc ttgcgcgccg tctggagcat ccgccgcatg
     1441 gcgggcgact cgccgatgat ctggtcgaag ccgaactcgc gcgcacgctc gcggcgcatc
     1501 tgcccgattt cacggcggag ctgatgggcc tcgatgccgt tgttgatcct cactcggagt
     1561 tcgtcgagat tggggggctt ggcgacgaag tcgtacgcgc cgccgcgcag cgccgcgatg
     1621 gtgttctcga cgagcacgtt ggccgtcacc atgatgacga ccgcctcggg atggcgaccc
     1681 ttgatctcgc gcagcacgtc gaggcccgac ccgtccggca gctcgatgtc gagcagcacc
     1741 gcggcggggc gctcgtgctc gtaagcgctg atagcgtcgg cgaccgtccc cgcctcgatg
     1801 ggcacgtagc cccacccccg caacgtctgt gcgaacgacc aacgtatcat ctgctcgtcg
     1861 tcaacgacga gcaccttttc cccagtcgtt ttcagtcgtc ctccttgggt ggcgaaaccg
     1921 cctgaccgcg tggaaggttc aagcgcgagg tgagctgttg gtgtgggcca gtataacgcg
     1981 cgggcggtgg gtgtagaaga ctcgccctcg ctcgtgagga tttttgattg ggaaattgtg
     2041 tgagcactac ggcgtactcg cgagccgcgc cagcaacatc ccgacgacat atgccagcga
     2101 agcggccagc ccgccgacag acagggttga caggcccgac ctccaccaag aggcggttga
     2161 ccacttgctc ttcgccgagc cgatggcgaa gaacacgccg cccgtgagca gaatcgaaag
     2221 ccggagcgcg tcgcgcccgc cgaagagaaa cgggacgagc ggcaccagcc cgcacaggac
     2281 gaacgcgctg aaggtggcga gggccgcgcg ccacggcgag cggatgtcgc cgggcagccc
     2341 gtactcttcc gtcagcatcg ttcgcaccca gcgcgacctg tcagaagtga tgagcgcgac
     2401 cacgcgctca agctcgcggc cttcgaagcc cttgccccgg tagatctgcc gcacctcctc
     2461 gcgctcgccg tcgggcacga cctcgatgtg ccggcgctcg accgattcca gccgctcgaa
     2521 gtcctcgcgc tcggcgcgcg tgccgaggaa gttgctcgcc gccatcgaga agccgtccgc
     2581 ggcgaggttg gcgaagcc
//
LOCUS       DBNGOH010000290         5810 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2243862, whole genome shotgun sequence.
ACCESSION   DBNGOH010000290 DBNGOH010000000
VERSION     DBNGOH010000290.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5810)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5810)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5810
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2243862"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            20..2260
                     /locus_tag="AC159P_05540"
     CDS             20..2260
                     /locus_tag="AC159P_05540"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014896.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HIR3694950.1"
                     /translation="MPSRPFVAALVVLTVCALFTVVPVYGRQPPEEVRGGARLGYPPA
                     RRVGQVDTYHGVEVADPYRWMEETDSEEVRGWVKAQDGLLKSFVGEAPGRAALQQRLA
                     ELTRYDMYAAMRFVGPEFEKRGRQYFFIKTSAGASQPALYVQAGLMSEPRTLLDLRAR
                     RGDDALRLAGFSPSPDGRLVAYLSSRNLSNWLSLRLLDVATGRELPETLEGLHFSGGT
                     ITWAPDGKGFFYTSFERPSEGTERRAPVRNAKIYFHALGTEQSSDRLVHERTEEPGLL
                     YSHRVTDDGRYLVVTATEGGGNKSRILYADLRSPGGPLKTLIGEADADYKLLGGRGAR
                     LWFYTDAQAPRGRVVAIDVARPQRGRWVEVIPEAGEAISAGSSVGGNALGLYGDRFVI
                     VYIRDGRPLLRIFDLRGRLRREAGFPEGGSIWGGFSGGPRDSEVFYRFLELASPGTIY
                     RLNVLTGKTNVFRRATVKVNPDDFVVERVFYRSKDGTRVPMFVAHKRGARLDEDTPAY
                     LYGYGAFAWVAFTWFQPHMIAWMEHGGVFAVASIRGGGEYGAAWHEAGSRLNKQKAID
                     DYVAAAEWLVDKRYTSRRRIVANGGSASGAVAAAAILQRPDLFAAGVIDIPVLDMLRF
                     DKFTAASYWKPEFGSPADPREFRALYAYSPYHNVRPGQCYPPTLVMAGERDQTAVPLH
                     AYKFVAAMQAAQACAYPVLLKVMWGTGHNFGATPERSVDSWTDLLSFLFRTLDLKPPS
                     ETARGG"
     gene            complement(2278..4014)
                     /locus_tag="AC159P_05545"
     CDS             complement(2278..4014)
                     /locus_tag="AC159P_05545"
                     /inference="COORDINATES: protein motif:HMM:NF012698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     tetratricopeptide repeat protein"
                     /protein_id="HIR3694951.1"
                     /translation="MRYEFDEYSLLTSERLLLRRGERVQLKPKVFDTLLALVRNPGRL
                     LSKEELIRLIWPDSFVEVSNLSQNIFTLRRVLGESPQDHRFIVTVPGYGYRFVAKVRE
                     LNEGEGPGGAAAAEVRPPIRSLAVLPLKSLLPDAGNVYLGLVIADTLITQLGAGGVVS
                     VRPTSTVLKYAETERDPLEVGRKLQVDAVLDGTVRRSGDRVKVNVQMIAVPGGETLWA
                     NRFEAAVADFAVIEDALAEQVAASLRVELERGAGTRPHRKTPKDIDVYQTYIKGRFFW
                     DKRTEEGLRVALSSARVVLEAEPELPLGYIGLADSYLLMGEYLYLSPGEAFPPALVAA
                     RKALALDPALGEAHASMAEYSFFHGRDWAGAERYYRDAIKLSPDYATARHWYTWFLIA
                     MSRYEDAAEQIRQAQMIDPGSLVLNTIVGLPYYYLGRYEQAIRQFRGTLEIEPKHHHA
                     RYYLGSALVHAGRPEEAVAEFEAMVAADPIQQAIALLGYCYAVSGRRREAGRMLSRLD
                     ELERRRFVAAYVRAFIHMGLGERARALDQLERAYEEQEPWLVFLNVDPFFRGLRGEPR
                     FTRLLERLRFPV"
     gene            4459..4860
                     /locus_tag="AC159P_05550"
     CDS             4459..4860
                     /locus_tag="AC159P_05550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694952.1"
                     /translation="MDFWVGEWDVTAQGQKVATSKISLIEAGCIVLEQYSQRDGYTGQ
                     SFNYYNPALGKWRQAWVDRGGNISEFSGEYKDGAMRYEGVSYPLGQAKVLRRMILFNL
                     GRDEVRQLSYRSADDGRTWHVNYDFLYVRKK"
     gene            4942..>5810
                     /locus_tag="AC159P_05555"
     CDS             4942..>5810
                     /locus_tag="AC159P_05555"
                     /inference="COORDINATES: protein motif:HMM:NF048867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kelch repeat-containing protein"
                     /protein_id="HIR3694953.1"
                     /translation="MVNKGRWLLPALLTKGSRCVLVLSTLLMFSSQPTTADRGVKTFP
                     RLGVRNAHAMTYDSGRGRVILFGGADESKVRGDTWEWDGRRWSLVSQAGPGPRTFPAM
                     AYDSLRKKSVLFGGNRVLFGKNPDENRFLNDTWEWDGERWTRIQAEGPSPRAEAAMAF
                     DSRRGRVVLFGGHDRAGEAGRRLGDTWEWDGKRWMEMKVTSPSPRNGAAQVYDVVRGN
                     IVLFGGSTQTGVSGETWEWDGKQWVENRAALTQGRFNCVMAYDSARRKVIRFGGRYAG
                     KPLGDTWEYDGKK"
BASE COUNT         1019 a         1832 c         1962 g          997 t
ORIGIN      
        1 aggagtttac atgcgtcgaa tgccatctcg tccgttcgtg gccgcgctcg ttgtcctgac
       61 cgtctgcgcc ctctttactg tcgtcccggt ctatggtcgg caacccccgg aggaagtgcg
      121 tggaggcgcg cggctcggct acccgcccgc ccggcgcgtg gggcaggtgg acacgtacca
      181 cggcgtcgaa gtggccgacc cgtaccgctg gatggaggag acggactcgg aggaagtccg
      241 cggctgggtg aaggctcaag acggactgct gaaatcgttc gtcggtgagg cgccggggcg
      301 cgcggcgctc caacaacgtc tcgccgagtt gacccggtac gacatgtacg cggcgatgcg
      361 cttcgtcggg ccggagttcg agaagcgcgg gcgtcaatac ttcttcatca agacgagcgc
      421 cggcgcgagc cagcccgcgc tctacgtcca ggcgggcctt atgtccgagc cgcgcacgct
      481 gctcgacctc cgcgcgcgcc gcggcgacga cgcgcttcga ctcgcgggct tctcgccgag
      541 tccggacggg cgtctggtcg cctacctgtc gtctcggaat ctatcgaact ggttgagcct
      601 gcgcttactc gacgttgcca cgggccggga gctgcccgag acgctggagg gcctgcactt
      661 cagcggcggg acgatcacgt gggcgcccga cgggaagggc ttcttttaca ccagcttcga
      721 aaggccgtcc gaggggacgg agcgacgcgc ccccgtcagg aatgcgaaga tatactttca
      781 cgcgctcggg acagagcagt cgtccgaccg gctcgtccac gagcggacgg aggagccggg
      841 cctgttatac agtcatcgag tcaccgacga cgggcgctat ctcgtcgtca cggcgaccga
      901 gggcggcggc aacaagagtc gtatcctgta cgcggatttg cgctcgccgg gcggcccttt
      961 gaagacgctc atcggcgaag ccgacgcgga ttacaagctg ctcggcgggc gcggcgcgcg
     1021 tctctggttt tacaccgacg cgcaggcccc gcgcggtcgc gtcgtcgcta tagacgtcgc
     1081 ccgcccgcag agagggcggt gggtcgaagt catcccggag gccggcgaag ccatttcggc
     1141 gggcagcagc gtcgggggca acgcgctggg gctttacggc gaccgtttcg tcatcgtcta
     1201 catccgcgac ggtcgccccc tcctgcgcat cttcgacctg cgcgggaggt tgcggagaga
     1261 ggccggcttc cccgaaggcg gctccatctg gggcgggttc tccggcggcc cgcgcgactc
     1321 ggaagtcttc taccgctttc tcgaactcgc cagccccggg accatctacc ggctgaacgt
     1381 cttgaccggg aagacgaacg tcttccgccg cgccacggtg aaggtgaacc ccgacgactt
     1441 cgtggtcgaa agggtcttct accggagcaa ggacgggacg cgggtgccga tgttcgtcgc
     1501 gcacaagcgc ggcgcccggc tcgacgagga cacccccgcc tacctctacg gctacggcgc
     1561 gttcgcctgg gtcgccttca cctggtttca gccgcacatg atcgcgtgga tggagcacgg
     1621 cggcgtcttc gccgtggcct ccatccgcgg cggcggcgag tacggcgccg cctggcacga
     1681 ggcgggcagc aggctgaaca agcagaaagc gatcgacgat tacgtcgcgg ctgccgagtg
     1741 gctggtcgat aagcgctaca cgtcgcgccg ccggatcgtc gccaacgggg gcagcgcgag
     1801 cggggcggtg gctgccgcgg ccatcctcca gcgccccgac ctcttcgccg caggcgtcat
     1861 agacatcccc gtcctcgaca tgctgcgctt cgacaaattc accgccgcct cctactggaa
     1921 accggagttc ggctcgcccg ccgacccgcg ggagttcaga gcgctctacg cctactcgcc
     1981 ttaccacaac gtcaggcccg gccagtgcta cccgccgacg ctggtcatgg ccggcgagcg
     2041 cgaccagacc gccgtcccct tgcacgccta caagttcgtg gcggcgatgc aggcggccca
     2101 agcgtgcgca tacccggtgc tgctcaaggt gatgtggggc acggggcaca acttcggggc
     2161 gacgccggag cgcagcgtcg attcgtggac agacctcttg tcgtttctct tccgcaccct
     2221 cgacctcaag ccgccgtccg aaaccgcccg cggcggatag cacgccgccg aataggctca
     2281 gaccgggaac cgcagacgtt cgagtaagcg tgtgaagcgc ggctcgcccc gcaggccccg
     2341 gaagaacggg tctacgttca agaacacgag ccacggctcc tgctcctcat aggcgcgctc
     2401 aagctggtcc agcgcgcgcg cccgctcgcc cagccccatg tgtatgaagg cgcggacgta
     2461 tgccgcgacg aagcgtcggc gctccagctc gtcgaggcgc gacagcatcc ggcccgcctc
     2521 tcggcggcgg cccgagacgg cgtaacagta gccgagcagg gcgatggcct gctgtatcgg
     2581 gtcagccgcg accatcgcct cgaactccgc gaccgcctcc tcgggtctgc cggcatggac
     2641 gagcgcggag ccgaggtagt agcgggcgtg atggtgtttg ggctcgattt cgagagtgcc
     2701 gcggaactgt ctgatggcct gctcgtaacg ccccagatag tagtacggca gcccgacgat
     2761 ggtgttgagg acgagggagc cggggtcgat catctgcgcc tgcctgatct gctccgccgc
     2821 gtcttcgtag cggctcatcg cgatcaggaa ccaggtgtac cagtggcgcg cggtggcgta
     2881 gtcggggctg agcttgatgg cgtcccggta atacctttcg gcccctgccc agtccctccc
     2941 gtggaagaac gagtactcgg ccatcgaggc gtgggcctcg cccagcgccg ggtcgagcgc
     3001 gagcgccttg cgtgcggcca cgagcgcggg cgggaaggct tcgcccggcg aaaggtagag
     3061 atactcaccc atcaggaggt aggagtccgc caggccgatg tagccgagcg gcagctcagg
     3121 ctccgcctcc agcacgaccc gtgccgagga gagcgcgacg cgaagcccct cttccgtccg
     3181 cttgtcccag aagaaacggc ccttgatgta tgtctggtaa acgtcgatgt ctttaggggt
     3241 cttccggtgc gggcgggtgc cggccccgcg ctccagctcg acccgcaacg aggcggccac
     3301 ctgctcggcg agcgcgtcct cgatgacggc gaagtcggcc accgcggcct cgaagcggtt
     3361 cgcccacaac gtctcgccgc ccggcacggc aatcatctgc acgttgacct tcacgcggtc
     3421 gcccgacctg cgcaccgtgc cgtccaggac agcgtctacc tgtagcttcc gccccacctc
     3481 cagggggtct ctttccgtct ccgcgtactt cagcaccgtc gaggtcgggc ggacggagac
     3541 gaccccgccg gcgccgagtt gggtgattag agtgtcggcg atgaccaggc ccagatagac
     3601 gttgccggcg tcgggcagca gggacttcag cggcaggacg gcgagcgacc tgatgggcgg
     3661 cctgacctcg gcggctgcgg ccccgcctgg gccttctccc tcgttcagct cgcgaacctt
     3721 ggcgacgaag cggtagccgt agccggggac ggtcacgatg aagcggtgat cctgcgggct
     3781 ttcgccgagg acccttcgca gggtgaagat gttctgcgag aggttgctca cctcgacgaa
     3841 gctgtcgggc cagatgagcc tgatcaactc ctccttgctc agcagccgtc cggggttccg
     3901 gacgagcgcg aggagcgtgt cgaacacctt cggtttgagc tggacgcgct cgcctctcct
     3961 gagcagcaga cgttccgagg tcagcaggct gtactcatcg aactcgtacc gcacgaaatc
     4021 ctctcccgtc tcgacgcgtt aacgaagaat gagaaaaaat aacagccccg agggcgcccg
     4081 acgtgctaga gtgcgcgccc tcgcctgcaa gcggcgcgaa gggtttcggc cctcggcccg
     4141 accgcgcgga agttccagtc tacccgaacc gaccgtcgtc agcgcaggaa cttcgatgca
     4201 tcaacctgcc gacctgtttt taccccgcgc ggcactagag gccggcgcgg cggggtgatg
     4261 tacatgagac tcttcgcctg cattgttttc ttatgcgtac aacagctctc ggtatcgggt
     4321 gtgtggcagg caccggccag cgaagcgccc gcgtcggccc gtgcgggagg ccgagcactc
     4381 gcgcgcaggt cgccgttcgt cgagagcgca cagcggcccg gtgaacagtc caggccctgt
     4441 gggaaggaac acaggcagtt ggatttttgg gtcggcgaat gggacgtgac cgcgcagggg
     4501 cagaaggtcg ccacgagcaa aatcagtctg atcgaagcgg gctgcatcgt ccttgagcag
     4561 tattcccagc gtgacggtta cacggggcag agcttcaatt attacaaccc cgcactcggc
     4621 aaatggcgac aggcttgggt ggacaggggt gggaacatca gcgagttcag cggcgagtac
     4681 aaggacggcg cgatgcgcta cgaaggcgtc tcataccccc tgggccaagc caaggtgctc
     4741 cggcgcatga tcctgttcaa tctcggtcgt gacgaggtca gacagttgtc ctaccgctcg
     4801 gcggatgacg gacgaacctg gcacgtcaac tacgatttcc tctacgtcag aaagaagtag
     4861 gcttgcgggt tctgccgtga gaaccgcggt cgcgttgcga aaccgccgga gcgcagccat
     4921 gtaatcatcc aggagttgat gatggtaaac aaagggcgct ggctgttgcc tgccttatta
     4981 acgaaaggtt cgcgatgtgt tctcgttcta tcaaccctgc tcatgttctc gtcgcagcca
     5041 actactgccg atcggggagt gaaaacattt ccgcggctcg gtgtcagaaa cgctcacgcc
     5101 atgacatatg acagcggtcg tgggcgagta atactctttg gcggcgcgga cgagtcgaaa
     5161 gttcgtggtg acacgtggga gtgggacggc aggaggtggt ctctggtgag tcaagccggc
     5221 ccggggccgc gaacatttcc tgctatggct tatgacagtc ttcgcaaaaa atcggttctt
     5281 ttcgggggca accgagtcct cttcggcaag aaccctgatg agaacaggtt tctgaatgat
     5341 acgtgggaat gggacggcga gaggtggaca cggatacagg cggaggggcc gtcgccccgt
     5401 gcggaagctg caatggcatt cgacagcagg cgtggacgcg tcgtgctctt cggcggacac
     5461 gaccgcgccg gagaagcggg gcgtcgactc ggagacacgt gggagtggga cggcaagagg
     5521 tggatggaga tgaaggttac aagtccctcc ccgagaaacg gtgcggcgca ggtctatgat
     5581 gtcgtgcgag ggaacatagt tctcttcggt ggctcgacgc aaacaggtgt ttcgggcgag
     5641 acgtgggagt gggacggtaa gcagtgggta gagaatcgag cggccttaac tcaaggccgc
     5701 ttcaactgcg tcatggctta tgacagcgcg cggcggaagg tgattcgctt tggcggtcgt
     5761 tacgcgggga aacctttagg cgatacctgg gaatatgacg gcaaaaaatg
//
LOCUS       DBNGOH010000291         6082 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3710018, whole genome shotgun sequence.
ACCESSION   DBNGOH010000291 DBNGOH010000000
VERSION     DBNGOH010000291.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6082)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6082)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6082
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3710018"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1020)
                     /locus_tag="AC159P_05560"
     CDS             complement(<1..1020)
                     /locus_tag="AC159P_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HIR3694954.1"
                     /translation="MSAKLGEILVRENLLTPQQLREALDFQREHGGRLGYNLVKMGLV
                     SDDMITAVLSRQYGVPSVNLELFDIDGSVIRLIPREVAEKYSVLPLSRVGATLTLAMV
                     DPTNVFAMDDIKFMTGLNIEPVVVSEASVQEAITSYYAQTREIELARAGGEDPYGGNG
                     NGNGNGYHHESPPEPEESLSLDSLDFDTEGAEGLEVVDENDEIDLSNLTRMSEDAPVV
                     RLVNVLLADALNRGASDIHVEPYEKELRIRFRIDGVLYDVMHPPMRLRDAMISRIKIM
                     SKLDIAEKRLPQDGRIKIKLRAHGRLRELDFRVSTLPTLFGEKVVLRLLDKERLMLDM
                     TRLGFE"
     gene            complement(1295..2647)
                     /locus_tag="AC159P_05565"
     CDS             complement(1295..2647)
                     /locus_tag="AC159P_05565"
                     /inference="COORDINATES: protein motif:HMM:NF025006.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3694955.1"
                     /translation="MFAKFLSRAASAALLLAAMSVAASAQVVQATGKVTLKQADGTEA
                     PVQNAVIKFYRTDIKQSFETKTSKNGTYVHAGIPLVGTFTIAVSAPGARPSYVTDVPI
                     SRRSENNFALEPGDGSALTLEDIKKLGASGGTAPAASGGAAAAPAKVSEEDKKRAAEA
                     AAERARIEEANAKAAELNTKLPEILKAGNDAFTAKKYDEAITHYDQGLALDPTQAVFH
                     RNRAIVLRARGVEKFNAAAKAKDVPGKEAAKEDFKNSAESMEKALAAQRDHAAKNAGA
                     AAPAGGGQPNDELGFLFDRSESYRVALQTGAAVNTESAVKAFEEYLAVETDPAKKMRG
                     QNGLGDALFMGGKMDESIATFRQVIATNPQNLDAMLGLGTALAAKGSLAEGGSGSAMI
                     AEARDLFQEFIKKAPDTHPKKADAVATAQYLDETIKAAKEPKPSTGTGRTTGTGRRRP
                     "
     gene            complement(2792..3445)
                     /gene="queC"
                     /locus_tag="AC159P_05570"
     CDS             complement(2792..3445)
                     /gene="queC"
                     /locus_tag="AC159P_05570"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005031869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - tRNA queuosine(34) biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HIR3694956.1"
                     /translation="MCLVSGGMDSCVTAALAREENEELAFLHVSYGQRTEARERRAFE
                     ELADFYRVTRRLAVSLEHLARIGGSSLTDTSIPVAAANLSSREIPTSYVPFRNAHLLA
                     AATSWAEVIGAARVYIGAVAEDSSGYPDCRPEFYEAFQRAVDVGTKPSTRVEIRTPVI
                     HLRKSEIVRRGLALGAPLQLTWSCYREEERACGRCDSCALRLRAFAEAGAADPIAYA"
     gene            complement(3642..4286)
                     /locus_tag="AC159P_05575"
     CDS             complement(3642..4286)
                     /locus_tag="AC159P_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HIR3694957.1"
                     /translation="MLRVTEIFRSIQGESTHAGRPCSFVRLTGCPMRCVWCDSEYTFT
                     GGDHMTVDDVMRHVHDFDCPLVEVTGGEPLAQREALTLIRRLCEEGYEVLIETGGFVS
                     TEGVDPRAKIILDVKCPASGEAERNHWPNLERLNAARDEVKFVVANRADWDFTLEVVK
                     LYDLERRAHAVLVSPVWGETDLKELADWVAASGMQIRMQLQLHKYIWGADVHGV"
     gene            4532..5479
                     /locus_tag="AC159P_05580"
     CDS             4532..5479
                     /locus_tag="AC159P_05580"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR3694958.1"
                     /translation="MPRDIEQLLREGAARLVARAGEARALGADAVLPRVRATTEKYLL
                     RHQPDAPAKEVGEFIDRLHADEFLLVVACERGDERAWADLMERYRATVMSAARGASAG
                     EAEAEELAQSVWAELYGLRETKDGRPAGKLAYYSGCGSLGGWLRAVVGQLGVDRHRRT
                     SRLVQTEEASDFDRLAAAEGAHDDGWRPRALPDPESALAEGEAARAVEEALAQAVAEL
                     SGEDRLLVKLYYFDGLRLKEAGVVLGVHEATASRRLTRLHAEIRKRVETILVGERRWT
                     REETARNLSTAAHMEMGGDLRSMLATGKVAGEQREELYE"
     gene            5587..>6082
                     /locus_tag="AC159P_05585"
     CDS             5587..>6082
                     /locus_tag="AC159P_05585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694959.1"
                     /translation="MKQEFDKEMDSLLRRSARLAAGPQSAGAARAPAASSHLDADELS
                     AFAENALPSAARFAAASHLADCDECRGMVVQLAGAADTAGEIEKRAAAAAPYVPVAIP
                     WWKSLLSAAFAPRVLRYAAPVLVLGLVGVVSLITLRTRQEMAPTTVYQKGENADTGAA
                     NSPGQ"
BASE COUNT          978 a         1875 c         2160 g         1069 t
ORIGIN      
        1 ctcgaagccg agccgcgtca tgtcgagcat cagccgctcc ttgtcgagga ggcggagcac
       61 gaccttctcg ccgaagaggg tgggcaaggt cgagacgcgg aagtccagct cgcgcagccg
      121 accgtgcgcg cggagcttga tcttgatgcg tccatcctgc ggcaggcgct tctcggcgat
      181 gtcgagcttc gacatgatct tgatgcgcga gatcatggcg tcgcgcagac gcatcggcgg
      241 gtgcatcacg tcgtagagca cgccgtcgat gcggaagcgg atgcgcagct ccttctcgta
      301 aggctcgacg tggatgtccg aggcgccgcg gttgagcgcg tcggccaaaa gcacgttgac
      361 gaggcgcacc accggcgcgt cctcgctcat ccgcgtcagg ttcgagaggt cgatctcgtc
      421 gttctcatcg acgacctcaa gcccttcggc gccctccgtg tcgaagtcga gcgagtcgag
      481 cgagagcgac tcctccggct ccgggggcga ttcgtggtgg tagccgttgc cgttgccgtt
      541 cccgttgccg ccgtaggggt cttcgccgcc agcgcgcgcc agctcgatct cgcgcgtctg
      601 cgcgtagtag ctggtgatcg cctcctggac gctcgcctcc gagacgacca ccggctcgat
      661 gttcagcccc gtcatgaact tgatgtcgtc catggcgaag acgttcgtcg gatcgaccat
      721 cgccagcgtc agcgtcgcgc cgacgcgcga gagcggcagc accgaatact tctcggccac
      781 ctcgcgcggg atgaggcgga tgaccgagcc gtcgatgtcg aacagctcga ggttgacgct
      841 gggcacgccg tactgccgcg aaaggacggc ggtaatcatg tcgtccgaca ccagccccat
      901 cttcacgagg ttgtagccga ggcgcccgcc gtgctcgcgt tgaaagtcga gtgcctcgcg
      961 caactgctgc ggggtcagta ggttctcgcg gacaagaatt tcgcctagtt tggccgacat
     1021 ttcagcagaa gttccagagc tgttagctgc tttgaaaagg ggcgggcgac cctgcgggcc
     1081 gagagtaaac gggaaaacac cgacctgttc accatcgggc aacgatggca aacaccgttg
     1141 gccagcagta tgttactggt aaaccacgcg ggctgtcaag atgattgtgg aagactgtcg
     1201 atatgggttc aggaacgcgg aaatgccaat aaaatagccg cgcctgattt ggcgcggcta
     1261 ttttattggg ccgggtcttt aacggactga ggggttacgg cctgcggcga ccggtgcccg
     1321 tcgtcctgcc ggtgccggtg ctcggcttcg gctctttggc ggccttgatc gtctcgtcga
     1381 ggtattgcgc ggtggccacg gcgtctgcct ttttcgggtg cgtgtccggg gccttcttga
     1441 tgaactcctg aaagaggtcg cgcgcttcgg cgatcatggc gctgccgctg ccgccttcgg
     1501 cgaggctgcc tttggcggcg agggccgtcc cgaggccgag catggcgtcg aggttctgcg
     1561 ggttggtggc gatgacctga cggaaggtgg cgatggactc gtccatcttg ccgcccatga
     1621 agagcgcgtc gccgagaccg ttctggcccc gcatcttctt cgccgggtcg gtctcgaccg
     1681 cgaggtactc ctcgaaggct ttgacggcag actccgtgtt cacggcggcg ccggtctgga
     1741 gggcgacgcg gtagctctcg gagcgatcga agaggaagcc gagttcatcg ttcggctgcc
     1801 cgccgcctgc gggggcggcg gcgcccgcgt tcttggcggc gtggtcgcgc tgtgcggcga
     1861 gggccttctc catgctctcg gcgctgttct tgaagtcttc cttggcagcc tctttgccgg
     1921 gcacgtcctt ggccttggcg gcggcgttga acttctcgac gccgcgggcg cgcaggacga
     1981 tggccctgtt gcggtggaag acggcctgcg tcgggtcgag cgccagtccc tgatcgtagt
     2041 gggtgatggc ctcgtcgtac ttcttcgcgg tgaaggcgtc gttgccggcc ttcaggattt
     2101 ccggcagctt ggtgttcagc tcggcggcct tcgcgttcgc ctcctcgatg cgcgcgcgct
     2161 ccgccgccgc ttccgccgcg cgcttcttgt cctcttcgct gaccttggcc ggggccgccg
     2221 cagcgccgcc ggaagcggcg ggggcggtgc cgcccgaggc gcccagcttc ttgatgtcct
     2281 ccagcgtcag ggccgagccg tcgcccggtt cgagcgcgaa gttattctcc gacctccggc
     2341 tgatcggcac gtccgtgacg taagacgggc gcgcgccggg cgcgctgacg gctatcgtga
     2401 aggtgccgac gagcgggatg ccggcgtgga cgtaagtccc gttcttgctg gtcttggtct
     2461 cgaacgactg tttgatgtcg gtgcggtaga acttgatgac ggcgttctgc accggagcct
     2521 ccgtgccgtc cgcctgcttc agagtcactt tgccggtggc ctgcaccacc tgcgcggaag
     2581 cggcgaccga catcgcggcc aggagcagcg cggcggaagc ggcgcgtgaa aggaatttag
     2641 cgaacatcgt cggtggtctc ctgaattgga attattccgg gcgctgtcaa aaagagtctg
     2701 aaaccgctgc ctatgatgct gatgcgccga cgccggttgt aagtttgatg ccacatgata
     2761 gcaggaaagc gggccggaat gcgaccccat ttcaagcgta agcgatgggg tctgcggccc
     2821 ccgcctcggc gaaggcgcgc agccgcaggg cgcacgagtc gcagcgcccg cacgcgcgct
     2881 cctcctcccg gtagcaagac cacgtcagct gcaaaggcgc gccgagcgcg agaccgcggc
     2941 ggacgatctc ggacttgcgc aggtggatga cgggcgtcct gatctcgacg cgcgtcgagg
     3001 gcttcgtgcc gacatcgacg gcgcgctgga aagcctcgta gaactcgggg cggcagtcgg
     3061 ggtagccgga cgagtcctcg gccacggcgc cgatgtagac gcgcgcggcg ccgatgactt
     3121 cggcccagct cgtcgccgcc gcgagcaggt gcgcgttgcg gaaggggacg tagctcgtcg
     3181 gtatctcgcg cgaagagagg ttggctgccg cgaccgggat cgaagtgtcc gtgagcgacg
     3241 agccgccgat gcgcgcgagg tgttcgagcg agacggcgag ccgccgcgtg acgcggtaga
     3301 agtcggccag ctcctcgaac gcgcgccgct cgcgcgcctc tgtgcgctgg ccgtaggaga
     3361 cgtggaggaa cgccaactct tcgttctcct cgcgcgccag cgccgccgtc acgcacgagt
     3421 ccatgccgcc ggagacgaga caaatagcaa gtgataagga ctgaggactg aggactgagt
     3481 ccgtctcgtc cgccggcccg tcgaatgctt tttcgtcgaa tccacccata acaattaacg
     3541 atgaatgcgg aatgatgaat gatgaacgac aggcagcttg aatgatgacg agcagcgtgc
     3601 ttctcattca tcattccgca atccgcattc tgcatttctt gtcagactcc gtgaacgtcg
     3661 gcgccccaga tgtacttgtg gagctgaagc tgcatgcgta tctgcatgcc gctggcggcg
     3721 acccagtcgg cgagttcctt gaggtcggtc tcgccccaga cgggggagac gaggacggcg
     3781 tgggcgcggc gttcgaggtc gtagagcttg acgacttcga gcgtgaagtc ccagtcggcg
     3841 cggttggcaa cgacgaactt gacctcgtcc ctcgcggcgt tgaggcgttc gaggttgggc
     3901 cagtggttgc gctcggcctc gccggaggcg gggcacttca cgtccaagat gatcttggcg
     3961 cgcgggtcaa cgccctcggt cgagacgaag ccgccggtct cgatcagaac ctcgtagccc
     4021 tcttcgcaga gccggcggat gagcgtcaaa gcttcgcgct gggcgagcgg ctcgccgccc
     4081 gtcacctcga cgagcgggca atcgaaatca tgcacatgcc gcatcacatc atcgaccgtc
     4141 atgtggtcgc cgcccgtgaa cgtgtactcg ctgtcgcacc agacgcagcg catggggcag
     4201 cccgtcaggc ggacgaacga gcacgggcgc ccggcgtgcg tggactcgcc ctggatggag
     4261 cggaagattt cggtgacgcg aagcataaga acagtaggca gtaagcagta tgcagcaggc
     4321 agtggaaaaa cgatctcagc cgaacggtca gggttcaagc gtcaacacga gtgacgcggc
     4381 tcctgctgct tactgcttac tgcctactgc ctactgttct tcatcattca tcattcgtcc
     4441 cggcgtgtca aagtatggag aaacggggcg gcgttcgtgg tagcatatcg tttccggcaa
     4501 gcgggcgttg ttgaggctaa agagcggtag gatgccgcga gacatcgaac aacttttgcg
     4561 ggagggcgcg gcgcggctcg tcgcgcgcgc gggcgaggcg cgcgcgctgg gcgccgacgc
     4621 ggtgctgccg cgcgtgcgcg cgacgacgga gaagtatctg ctgcgccacc agccggacgc
     4681 cccggcgaaa gaggtcgggg agttcatcga ccgcctgcac gcggacgagt ttctgctggt
     4741 cgtggcgtgc gagcgcggcg acgagcgcgc gtgggccgac ttgatggagc gctaccgcgc
     4801 gacggtgatg tcggcggcgc gcggcgcgag cgcgggcgag gccgaggccg aagagctggc
     4861 gcagtccgtg tgggccgagc tgtacggctt gcgcgagacg aaggacggga ggccggcggg
     4921 caagctcgca tactattcgg ggtgcgggtc gctcggcggc tggctgcgtg cggtggtggg
     4981 gcaactcggc gttgaccgtc accggcgcac gtcgcggctg gtgcagaccg aagaggcttc
     5041 ggacttcgac cggctcgccg ccgccgaagg cgcgcacgac gatggctgga ggccgcgcgc
     5101 gctgcccgac ccggagagcg cgctcgcgga aggcgaggcg gcgcgcgccg tcgaagaggc
     5161 gctggcgcag gcggtcgccg agctgtcggg cgaagaccgt ctgctggtga agctctacta
     5221 cttcgacggg ctgcgtctga aggaagcggg cgtggtgctc ggcgtgcacg aggcgacggc
     5281 gagccgtcgg ctgacgcggc tgcacgcgga gatacgcaag cgcgtcgaga cgatactcgt
     5341 gggcgagcgc cgctggacgc gcgaagagac ggcgcgcaac ctatcaaccg cggcccacat
     5401 ggagatgggc ggcgacctgc gctcgatgct cgccacgggg aaggtcgcgg gcgagcagcg
     5461 cgaagagctt tacgagtgag cttaccggtc gtgcggcgca agattgaggg cggttacgcg
     5521 ttcaactgtt gacgccgaag cggtcaaccg tcgaagcgca aacgatgcgt ggttcgtgaa
     5581 gttttgatga agcaggaatt cgacaaggag atggactcgc tgctgcgccg cagcgcacga
     5641 ctcgcggcgg ggccgcagag tgcgggcgcg gcgcgcgcgc cagccgcttc gtcgcacctc
     5701 gacgcggacg agctgagcgc gttcgcggag aacgcgctgc cttcagcggc gcgcttcgcg
     5761 gcggcctcgc acctcgcgga ctgcgacgag tgccgcggca tggtcgtcca actcgcgggc
     5821 gcggcggaca cggcgggcga gatcgagaag cgcgccgccg ccgccgcgcc ctacgtgccg
     5881 gtcgcaatcc cgtggtggaa gtctctgctg agcgcggcct tcgcgccgcg cgtgttgcgt
     5941 tatgcggcgc ccgtgctcgt actcggcctg gtcggcgtcg tctcgctcat cacgctccgc
     6001 actcgtcagg agatggctcc cacgaccgtc tatcagaaag gcgaaaacgc ggacacgggc
     6061 gccgccaact cgccaggtca gg
//
LOCUS       DBNGOH010000292         4808 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_227635, whole genome shotgun sequence.
ACCESSION   DBNGOH010000292 DBNGOH010000000
VERSION     DBNGOH010000292.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4808)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4808)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4808
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_227635"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..474)
                     /locus_tag="AC159P_05590"
     CDS             complement(<1..474)
                     /locus_tag="AC159P_05590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694960.1"
                     /translation="MRKATPKKSAPAAKASRTAPKQSKSAAKPKSVAAKARAAATTAP
                     ARKKAPAPSKASAKKPAPAKTAPTNKSKAAPPTKPSQRPAPAPPRQPSRDEAAALKAF
                     ERAHKEFARGRFVEARNLFRALIEQHAGVSEVTARARTYLAIADARMRSEAALPRD"
     gene            989..1471
                     /locus_tag="AC159P_05595"
     CDS             989..1471
                     /locus_tag="AC159P_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015722651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HIR3694961.1"
                     /translation="MTDTPIILHEQQYQQLRHILNKLRFESSARVVFLVDKNGQQIAV
                     QGEVGDLDTTSLASLAAGNVAATGGMAQLIGEKEFPTLSHEGERESIHICIIGRVLLV
                     VVFDERTSLGLIKLRVKQASRDLAALFEEVARVAAEKKHSSEAFFAEITDDDIDSLFN
                     "
     gene            1569..2153
                     /locus_tag="AC159P_05600"
     CDS             1569..2153
                     /locus_tag="AC159P_05600"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HIR3694962.1"
                     /translation="MTFINYASREINCKIVYYGPGLCGKTTNLQYIYDSTAPTSKGKL
                     ISLATETDRTLFFDFMPLELGTVRGFKTRFHLYTVPGQVFYDASRKLILKGVDGVVFV
                     ADSQEERMDANIESLYNLEENLTANGYDLTKIPYVLQLNKRDLPNVIPVEELVAELKR
                     KDEPVHEAVAATGVGVFDTLKSVAKQVLTELRKS"
     gene            complement(2248..2820)
                     /locus_tag="AC159P_05605"
     CDS             complement(2248..2820)
                     /locus_tag="AC159P_05605"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3694963.1"
                     /translation="MAVLKKIRSRLFSSGANGEGADEGRYVEGARAASRSALAEAGYD
                     VRPLTVAQLDECWRLDLRCFIDGEAYSRETFEYLLTSPDSVSYRAVTPEGAMVGFIVG
                     LVEPDFTGHITTLGVAPEYRRRGIANRMLVKAEDGFRRRNVRIVRLEVRSVNHGAQDL
                     YRKLGYAVTQRLPRYYSNGGDGLLMVKSLV"
     gene            complement(2956..4014)
                     /gene="queA"
                     /locus_tag="AC159P_05610"
     CDS             complement(2956..4014)
                     /gene="queA"
                     /locus_tag="AC159P_05610"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007779757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HIR3694964.1"
                     /translation="MFISDFDYELPDELIAQHPLGRRDASRMLVVERARGAWRDSSFS
                     EFPAELRAGDALVVNNTRVFPARLLGRREPTGGRVELLLIRQLEEAGEHVWEALARPA
                     RRLDAGARVAFGDGRLRAEVLEAPGGGPGRVVRFECEGDIDALVEEFGRMPLPPYIKR
                     ERDDLAAREEDRERYQTVYAERRGAIAAPTAGLHFTPRVLDELRARGVRVAEVTLHVG
                     YGTFAPVRAEDLKDHSVAAERFEIEDGAARTIHDARAGGGRVVAVGTTTVRALESATD
                     PSGHVRPGRGETGLTITPGYEFRAVDALLTNFHLPRSSLLFLVAAFAGRELALEAYRH
                     AVRARYRFYSYGDCMLIV"
     gene            complement(4095..>4808)
                     /locus_tag="AC159P_05615"
     CDS             complement(4095..>4808)
                     /locus_tag="AC159P_05615"
                     /inference="COORDINATES: protein motif:HMM:NF015587.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HIR3694965.1"
                     /translation="AGSEALARIVEVYPGSREFLRTTVRSLANVGWGGVLSACVVVLW
                     AGSWVFAVIERALNRLWGTKPRAFLHGRALTLGMIGSVGLLLILSVFLTSILVGLQQL
                     IDQLPIRVVSRSPTLALVGNAFWQTLFTIASTIVTITLFALVYRLVPNGRVTLRDTLP
                     SAIVAGLLWETAKYIFAWSLQYFHYDQIYGSVGAVVAVLTWSYVSSLVLMFGAQLSVV
                     LHQEHHVAEMAAETPASPS"
BASE COUNT          873 a         1536 c         1598 g          801 t
ORIGIN      
        1 gtcgcgcggc agcgccgcct ccgagcgcat ccgcgcgtcg gcgatggcga gataggtgcg
       61 cgcgcgcgcc gtcacttccg agacgcccgc gtgctgctcg atgagcgcgc ggaagagatt
      121 gcgcgcctcg acgaagcggc cccgcgcgaa ctccttgtgc gcgcgctcga aagccttcag
      181 ggccgcggcc tcgtcgcgcg agggctggcg cggcggcgcg ggcgcaggtc tctgcgaggg
      241 cttcgtcggc ggtgccgctt tggatttatt ggtcggagcc gttttggccg gcgcgggctt
      301 cttcgcactc gctttggagg gggcgggcgc cttcttcctg gcgggcgcag tagtagcagc
      361 cgcgcgcgcc ttcgcggcga cgctcttcgg cttggccgcg ctcttggact gtttcggtgc
      421 ggttcgtgaa gccttcgcgg cgggcgccga cttcttcggc gtggctttac gcacgggcgc
      481 cttctccgcc gccgagcgct tcggcttggg ccgaggttga gcggacttgc cgctcacttt
      541 acctttagcc gaagactttg aagaggcgct actcggtgca gggcgggagg tgtttctctt
      601 tgcacctttg atcacgggat cgctcatgcg aagcactctg gttttcacag atagcagcaa
      661 aggggccgcc accgaacgcc gccactttac cacacgggat ggtttaaatc aacgcaggag
      721 tgtggtttgc gggcactcgg cggcctttga aaggtctcct cggcggccaa catggaaaaa
      781 cagttgaaga cattccgagc gctatgctag agtgttttac gagttgcgac gaatgatcgt
      841 cgccccccgg ctcgactgac aagctggcct agcgaacgag ccaagtcttc tcaactcttc
      901 atttaagttc tcaggcggac gcccacttgc aagccccttt gcaactcacc ttctgtccgc
      961 aaccaagaga gaaagcgcct gtgtacgcat gacagacaca cccatcatat tgcacgagca
     1021 gcagtatcag cagctacggc atatcctgaa caagctgcgt ttcgagtcct cggcgcgcgt
     1081 ggtcttcctc gtggacaaga acggccagca gatcgccgtc cagggcgagg tgggcgacct
     1141 cgacacgacg agtctggctt ctctggcggc ggggaacgtg gcggcgacgg gtgggatggc
     1201 gcagctcatc ggcgagaaag agttccccac cctctcgcac gagggcgagc gcgagagcat
     1261 ccacatctgc atcatcggtc gcgtcctgct ggtcgtggtc ttcgacgagc gcaccagcct
     1321 cggcctcatc aagctgcgcg tcaagcaggc gtcgcgcgac ctcgcggccc tcttcgagga
     1381 ggtcgcgcgc gtcgccgccg agaagaaaca ttcgtcggaa gccttcttcg ccgagatcac
     1441 ggacgacgac atcgacagcc tcttcaattg aagtcagaag tcagaatgta taagtcagaa
     1501 tccagctttc gccgaattca cttccgacat ccgacatccg acttccgact tccgacttct
     1561 ataacgccat gactttcatc aactacgctt ccagagagat caactgcaag atcgtctact
     1621 acgggccggg cctgtgcggg aagacgacga acctgcaata catctacgac tcgacggcgc
     1681 cgacctcgaa gggcaagctc atcagcctgg cgaccgagac cgaccgcacg ctcttcttcg
     1741 acttcatgcc tttggaactc ggcacggtgc gcggcttcaa gacgcgcttc cacctctaca
     1801 cggtgccggg gcaggtcttc tacgacgcgt cgcgcaagct catcctcaag ggcgtggacg
     1861 gcgtcgtctt cgtcgccgac tcgcaggagg agcgcatgga cgccaacatc gagtcgctct
     1921 acaacctgga agagaatctg acggcgaacg gctacgacct gacgaagatt ccctacgtcc
     1981 tgcaactcaa caagcgcgac ctgccgaacg tcatcccggt cgaagaactc gtcgccgaac
     2041 tcaagcgcaa ggacgaaccc gtccacgagg ccgtcgccgc caccggcgtc ggcgtcttcg
     2101 acaccctgaa gtcggtcgcc aagcaggtct tgaccgagct gcgcaagagc tagaaaaggc
     2161 cgtaaaccgt aaaccgtgaa ccgtgataag aggaaagcag gttttcactt atcacggttc
     2221 acggtttacg gcttttaacg gcttttgcta gacgagcgat ttgaccatga gcaggccgtc
     2281 gccgccgttc gagtagtagc gcgggaggcg ttgggtgacg gcgtagccga gcttgcggta
     2341 gaggtcctgg gcgccgtggt tgacggagcg gacttcgagg cggacgatgc ggacgttgcg
     2401 gcggcggaag ccgtcctcgg ccttgacgag catgcggttg gcgatgccgc gccgacggta
     2461 ttcgggcgcg acgccgagcg tggtgatgtg gccggtgaag tccggctcga ccagcccgac
     2521 gatgaagccg accatcgcgc cctcgggcgt gacggcgcgg tacgagaccg agtcgggcga
     2581 ggtcagcagg tactcgaacg tctcgcggct gtaagcctcg ccgtcgatga agcagcgcag
     2641 gtccaaccgc cagcactcgt ccagctgcgc gaccgtcagc gggcgcacgt cgtagcccgc
     2701 ctcggccagc gccgagcgcg acgccgcgcg cgcgccttcg acgtaacgcc cctcgtccgc
     2761 gccctcaccg ttggcgcccg acgaaaacag cctcgaacgt atcttcttca gcacagccat
     2821 gccgggtaaa tggtaaatgg tgaatggtga atagtaaatg gtggacggaa gattaacggg
     2881 cgcgcggcct gaatgttctc gactcgagtc gaacgaccac tcaaccacca ttcaccatct
     2941 actattcacc attcactaga cgatgagcat gcagtcgccg tagctgtaga agcggtagcg
     3001 cgcgcggacg gcgtggcggt aggcttcgag cgcgagttcg cgacccgcga aggcggcgac
     3061 gaggaagagc agcgacgagc gcgggaggtg gaagttggtg agcagcgcat cgaccgcgcg
     3121 gaactcgtag ccgggcgtga tggtcagccc ggtctcgccg cgacccgggc ggacgtgacc
     3181 gcttgggtcg gtggcggatt ccagtgcgcg gacggtggtc gtgccgacgg cgacgacgcg
     3241 accgccgccg gcgcgcgcgt cgtggatcgt tcgggccgcg ccgtcttcga tctcgaaacg
     3301 ctcggcggcg acactgtggt ctttgaggtc ttcggcgcgg acgggcgcga acgtgccgta
     3361 gccgacgtgc agcgtgacct ctgcgacgcg gacgccgcgc gcgcgcagtt cgtccaacac
     3421 gcgcggcgtg aagtgcagcc cggcggtggg cgcggcaatg gcgccgcgcc gctcggcgta
     3481 gacggtctgg tagcgctcgc ggtcttcttc ccgcgcggcg aggtcatcgc gttcgcgctt
     3541 gatgtagggc ggcagcggca tccgcccgaa ctcctcgacg agcgcgtcga tgtctccctc
     3601 gcactcgaag cgcacgacgc gccccggccc gccgcccggc gcttccaaca cctccgcgcg
     3661 caaacgcccg tcgccgaacg cgacacgcgc gcccgcgtcg agcctccgcg cgggccgcgc
     3721 cagagcctcc cagacgtgct cgcccgcctc ttcgagttga cggatgagca gcagctcgac
     3781 gcgcccgccc gtcggctcgc gccggcccaa caaacgcgcc gggaagacgc gcgtgttgtt
     3841 gacgacgagc gcgtcgcccg cccgtagctc ggcagggaat tcggagaagg acgagtcgcg
     3901 ccacgccccg cgcgcgcgtt cgacgacgag catccgcgac gcgtcgcgcc gccccagcgg
     3961 gtgctgcgcg atcagctcat cgggcagctc gtagtcgaag tcggagatga acatgagaag
     4021 gagaagtcag aagtcagaag gatgaaggaa agcaccttgt ttttattcat cgttccgcgt
     4081 tctgacttct gactttagct cggcgacgcg ggggtctcgg cggccatctc ggcgacgtgg
     4141 tgctcctggt ggaggacgac ggagagctgc gcgccaaaca tgaggacgag gctcgacacg
     4201 tagctccacg tgagcacggc gacgacggcg ccgaccgagc cgtaaatctg gtcgtagtga
     4261 aagtattgga ggctccacgc gaagatgtac ttggccgtct cccacagcag gcccgcgacg
     4321 atggcgctcg gcagcgtgtc gcgcagggtg acgcgcccgt tcggcacgag gcggtagacg
     4381 agcgcgaaga gcgtgatcgt gacgatggtg ctggcgatgg tgaagagcgt ctgccagaag
     4441 gcgttgccga cgagcgcgag cgtcggcgag cggctgacga cgcggatcgg gagctggtcg
     4501 atgagctgtt gcaagccgac gagaatcgag gtgaggaaga ccgacaggat caggagcagc
     4561 ccgaccgagc cgatcatgcc gagcgtgagc gcgcgcccgt gcaggaacgc gcgcggcttc
     4621 gtgccccaca ggcggttgag ggcgcgctcg atgacggcga agacccacga gccggcccac
     4681 agcacgacaa cgcaggcgct caggacgccg ccccacccga cgttcgccag cgagcgcacg
     4741 gtcgtgcgca ggaattcgcg cgaccccgga tagacctcga cgatgcgcgc gagcgcttcg
     4801 ctcccggc
//
LOCUS       DBNGOH010000293        14308 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4993301, whole genome shotgun sequence.
ACCESSION   DBNGOH010000293 DBNGOH010000000
VERSION     DBNGOH010000293.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 14308)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14308)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14308
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4993301"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1184
                     /locus_tag="AC159P_05620"
     CDS             <1..1184
                     /locus_tag="AC159P_05620"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002612237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HIR3694966.1"
                     /translation="NGMTEDEQQALTEEMLRSGEVLDFSSIPRPKADKHSTGGVGDKT
                     SLVLAPLAAACGVVVPMISGRGLGHTGGTLDKLEAIPGYRVQLGLDEFRAILESVGFA
                     MMGQTSTIAPADRKLYALRDATETVEVVPLIVASIMSKKLAAGLDALVLDVKTGTGAF
                     MHDRERAAQLARALVQTGNSFGVRTEALITDMNQPLGHAVGNALEAKECVEILRGDAH
                     EKARPVLDLSVELAARMVNISGVEPSLEEARERVTQALASGAGLELFRRNVEAQGGDP
                     RVCDAPARLSDLTLTEVRVESPRRGLVAGVDAAEVGRAVADIGGGRARMEDEIDPAVG
                     FLAEARVGDEVKEGDVLGLLYCRDAAGGEEAAARIQAAYTVSSESPVRVLRLIKEVIT
                     P"
     gene            1181..2257
                     /locus_tag="AC159P_05625"
     CDS             1181..2257
                     /locus_tag="AC159P_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family lipoprotein"
                     /protein_id="HIR3694967.1"
                     /translation="MKFRVSLSLALVCALALSSGVACRYEGRVEGQADKVHVGIVFDI
                     GGKDDRSFNAAAYDGVKCAERGTTATGQPCEKPSLDIVLRDVEPGNPTSIEPAMRAFA
                     ESGYDLVIGVGFAQAPIMERVAKDYPRINFAIVDGVSQLPNVASLIFKEHEGSYLAGI
                     IAARASKTGVLGFVGGMDIPLIHRFETGYEEGARSVNPNVRVIDNYVGVTDGAWNNPG
                     KGKELALAQIDKGADVIFTAAGNSGLGVVDAVEQYGRDETGRARKFVIGVDSNQNMVK
                     PGFVLTSMVKRVDNAVYSIVEDVKNKRFKGGVHVYGLDADGVAYSLDQYNKPLIPAEV
                     LQEAEAAKQKIIAGEIKVTDAMNP"
     gene            2340..3866
                     /locus_tag="AC159P_05630"
     CDS             2340..3866
                     /locus_tag="AC159P_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015326437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3694968.1"
                     /translation="MLELRDITKRIGAVLANDGVSIKVETGTIHAIVGENGAGKSTAM
                     RIAYGFYTADSGEIVVEGEPRQIQTPHDAIRLGIGMVHQHFMLVDTMTVAENIVLGAE
                     PGTAAAIDLGKAAREIRKLSDEFRLSVDPGALVETLSVGQQQRVELLKALYRRARILI
                     LDEPTAVLTPQEVEEFFAILRRMREQGKTVVIITHKLSEVLAISDEVTVMRDGRVVGR
                     VKTSETDAGALARMMVGRDVLLRVEKPEPKVGGAVLNVERLSVAVHGVERGGLRDVSF
                     AVRAGEIVGIAGVEGNGQTELIEALAGLVPHAQVSGAVEFEGRDITRTGARQRRELGI
                     AHIPEDRHRRGLLLDFDLSENSILGDHYRPPAVTGGVLLNTGGIRGRAMEVIRDFDVR
                     PPNPDLPARALSGGNQQKLIIGREFELRPKLLLVSQPTRGVDIGAIEFIHRKLVSLRD
                     EGCAVLLVSAELEEVTSLADRLLIIYHGRIVGEVDPARATHEEIGLMMTGGEKEAVSR
                     "
     gene            3986..5113
                     /locus_tag="AC159P_05635"
     CDS             3986..5113
                     /locus_tag="AC159P_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3694969.1"
                     /translation="MKLLRELLFPLIAVAAAFVVGGVVVLLVGDNPLKVYGLLLGSAL
                     SWPDGIGETLFNATPLIFTGLAVAVAFRCGLFNIGAEGQLYVAAFLTAWVSIKFGGTV
                     ADVFGKPVDYSWASLPAVVLVPLALLTAIAAGAAWGAIPGVLKARFGAHEVITTIMLN
                     FIGVALVSYFTQYYYKVPGDPIMQTVSIGEAAHIPRLSAFVPGLPPHLSVNVAFIFAI
                     LACVFVYFFLWRTKWGYEIRATGANPTAAEYGGISTGRQIVVAMAVSGALAGLVAINE
                     VLGFRYRYYDGFSANYGFTGIAVALLGRNHPVGVFLAALLFGALIRGGIFVDAFSERV
                     SKDIVEVLQAVVILFVAAFMGAEAMLRSVFGRFGLLKRSKL"
     gene            5232..6113
                     /locus_tag="AC159P_05640"
     CDS             5232..6113
                     /locus_tag="AC159P_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008890746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3694970.1"
                     /translation="MDIFNLSLIYSSIRLTTPLLLAALGGLYSERAGVINIALEGLML
                     AGAFTAAAITHYAGSPWVGLLAGVVAGVVVAAIYAVVCIRFKADQVVSGTAINILMVG
                     VPAMLSGAFFFSSGSTPQIPKENLLPNTPIVIAFLLVPVTWYVLYRTPFGLRLRAVGE
                     NPGAADAAGVSVTKLRYSAVLLSGALAGIAGAYLSIGQSSLFTRNMTSGRGFIALAAL
                     IFGKWRPVQTMLACLLFGFTEAVAIQLQGVPIGGEEIPNQFIQIIPYVLTIVVLAGFI
                     GQSRAPRALGQPYQKER"
     gene            6110..6373
                     /locus_tag="AC159P_05645"
     CDS             6110..6373
                     /locus_tag="AC159P_05645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694971.1"
                     /translation="MKHTVEECTVVLRQLSGEWGVDEESVAVGSLEELYDYCLRLKEP
                     RLLERLVLAGRDERGRGRSLTFTFRSITDRNKTQAPDESEGRG"
     gene            6422..8092
                     /locus_tag="AC159P_05650"
     CDS             6422..8092
                     /locus_tag="AC159P_05650"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014811859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC1 kinase family protein"
                     /protein_id="HIR3694972.1"
                     /translation="MGILIKPEHLKRYKDIAWLLMKYGRADLVKTAGLEAAVEETEAD
                     GEGHALPAKAEELAADVEKLGPTYIKLAQLLSTRADMLPQAYLDALARLQDKVEPFSF
                     AEVEQIVSAELGVRMSKAFSDFESRPIAAASLGQVHRASLRDGRAVVVKVQRPGIREQ
                     ITEDMAALNEIAAFLDGHTEAGRRYEFTPILEEFRKTMLRELDYRVEAHNLLTFADNL
                     REFDRIVVPQPVEDFTTSRVLTMDYIKGRKITDLSPLAKIELDGYQLAEHLFQAYLQQ
                     LLVDGFFHADPHPGNVFLTGENRMIALLDLGMVGRLAPRFQEHLLQLLIAVSEGRGDE
                     AAEITVKIGEPKEEFDAEGFRRRVAELVIQHQTAKLENINAGRVVLQIARISGEHNFR
                     LPPEFTMISKTLLNLDQVVHTLDPKFDPNYSIRNYSTTIVQKRVRKAFSAGVMFGTAL
                     ELKEFVDKLPGRVNQLMDSVATKGLEIKIDAIDEVRLIEGIQKVANRITLGLLLASLI
                     VGAALLMRVETTFRIFGYPGIAIIFFLLAAGGALVLAFNILFNDEKRK"
     gene            8261..9091
                     /locus_tag="AC159P_05655"
     CDS             8261..9091
                     /locus_tag="AC159P_05655"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HIR3694973.1"
                     /translation="MVPETLYERAEHAARFIRARAKEDPRVALVLGSGLGAFADELED
                     AEAIPYEEIPGFARPTVEGHAGRLVVGGVGGAPVAAMQGRFHSYEGYSLEEVTFPVRV
                     LRLLGARTLVLTNAAGGLNNSYAQGSLIVISDHLNLMGVNPLRGPNDERFGPRFPDMT
                     SVYDHEYAETAIKEAREMNLELRRGIYAALPGPSYETPAEIRMFRLLGADAVGMSTVP
                     EAIVARHMSMRVLGISCITNMAAGVLDRPIDHAEVIETGERVRETFAELLRRVIPKLQ
                     "
     gene            9183..9857
                     /gene="udk"
                     /locus_tag="AC159P_05660"
     CDS             9183..9857
                     /gene="udk"
                     /locus_tag="AC159P_05660"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012188379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HIR3694974.1"
                     /translation="MIIGICGGTGSGKTTVANRILESVSKEEVGFLQQDSYYRDIDQL
                     PVDLRQQVNFDHPDAIDNDLLVEHLRALKRGEAVELPVYDFKNNARRAETLHLEPPAI
                     IIIEGILIFADERLRAEMDLKVFVDTPDDIRFIRRLRRDIAERGRTVESVCEQYEATV
                     RPMHMQFVEPSKRHADVIIPEGGHNLVSIDLISGRVRERIAAERRRRAAQRAGGGAAV
                     AAEQGG"
     gene            9858..10277
                     /gene="cdd"
                     /locus_tag="AC159P_05665"
     CDS             9858..10277
                     /gene="cdd"
                     /locus_tag="AC159P_05665"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HIR3694975.1"
                     /translation="MKTNTEQDIIRAATEARNCALAPYSNFCVGVAVEDEEGNIFTGC
                     NIESAAYELTMCAERVAIWKALSEGARKFRRVVVVADTERLTPPCGACRQLVWEFCGD
                     VPVVLANLRGDIEVLQMADLLPRPFDAQFLAETKAGE"
     gene            10274..10759
                     /locus_tag="AC159P_05670"
     CDS             10274..10759
                     /locus_tag="AC159P_05670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694976.1"
                     /translation="MREGVRLAMKGEGKEEEAHGGGKDGVEREIGEITPVEHFLNRYV
                     GYAIHLFLSLLAIFILAAALIATYQTLVYEFPKLLTPADEYKSLQSIIGHILLIAIAA
                     ELGLLLLFHRTSAAVEVIIFVIARKIVTPDISSLELLLSVAALAGLLVVRFYYLPGKP
                     K"
     gene            10756..12246
                     /locus_tag="AC159P_05675"
     CDS             10756..12246
                     /locus_tag="AC159P_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR3694977.1"
                     /translation="MKPHRLASTCLALLSIFMPVVGASAQRARGANRVDLIVRGGTVV
                     TMDGERRVIEDGAVAVERGRIVAVGRSAEIARRFTSAEVIDARGRAVIPGLVNGHTHI
                     PMTLFRGLGDDLDLNDWLTKYIFPAEAKNVTEEFVRTGTQLGLAEMIRGGTTTYCDMY
                     YFEDAIADETARAGVRAVLGETVIDFPVADNKTWGDAMRYVVRFVNKWKNHPLVTPAV
                     APHAPYTVSEEHLKDVRTFSDMTGAPIVTHVAETRKEVEDITKQKGVPPVEYLARIGF
                     LSERVIAAHTVHLNEREIGLLKEAGVGSVHNPESNMKLASGVAPVPRMLREGMRLGLG
                     TDGAASNNDLSMWEAMDAAAKLHKLTANDPKVVTAEEALMMATIGGARALHMEKEIGS
                     LETGKRADLVVVDLDALHQTPRYNIYSHLVYATKASDVRTVVIEGRVVMRDRRLLTLD
                     EALIKRKARAIRERVSRSLAAFSPGNGGKTPPPSRAAARRVDRKDE"
     gene            12392..13603
                     /locus_tag="AC159P_05680"
     CDS             12392..13603
                     /locus_tag="AC159P_05680"
                     /inference="COORDINATES: protein motif:HMM:NF015506.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HIR3694978.1"
                     /translation="MTLTVAVTGAQAQTQTTTEPAVTSNAATPTPAPTPTETEAVQRR
                     IARARALAAVGRLAAAAGELESLRAASTDESVRDVARLLLMWIFVEMPDYARANALLD
                     ETYKERGTEAARVSYHALAGQTINGVRAHLERYRTFGINVADADLPAEAVTDLDQLRG
                     LLERLVEHAKTMRGEEDAKSTAARSTETTALLEDAASVRLRLARNDQERTRWQGEVSE
                     ARQRLVASETRIASISSVPFSRPTPAPPTTTAPSPSVSDAPGRGAPAKTADRQKTAAP
                     ERAANGSANNSTASNAPAASQNPGQASATPAAGERQPVAIGSLHTVARQKSAPSYPSI
                     ARAARVTGVVTVFLVVNEKGEVESVTRADGPQQLQNAASDAARRWKFHPTVIDGQPVR
                     VTGYISFNFAL"
     gene            13632..14024
                     /locus_tag="AC159P_05685"
     CDS             13632..14024
                     /locus_tag="AC159P_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007920689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HIR3694979.1"
                     /translation="MTYALWIVQALLALLFLFAGGTKLVIPPEVLASMGSPNQIPLPG
                     WFVRFIGVAEVLGALGLILPGLLRIKPWLTPLAAVGLLIILIGATALSFAADGIAAAV
                     VPLVVGLLAAFVAYGRWRLAPHAGRGEK"
     gene            complement(14090..>14308)
                     /locus_tag="AC159P_05690"
     CDS             complement(14090..>14308)
                     /locus_tag="AC159P_05690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694980.1"
                     /translation="WPLFMVAYMLALAYVARRALSRLRSFRLSKRQGAPACSTGCGAC
                     GEAKQPARPAPEFVRISPSAKTPRRGTH"
BASE COUNT         2418 a         4889 c         4772 g         2229 t
ORIGIN      
        1 tgaacggcat gacggaggac gagcagcagg cgctgaccga agagatgctg cgctcgggcg
       61 aggtgctgga cttctcttcg atcccgcgcc cgaaggccga caagcactcg acgggcggcg
      121 tcggcgacaa gacctcgctc gtcttggcgc cgctggccgc cgcgtgcggc gtcgtggtgc
      181 cgatgatctc gggccggggc ttgggccaca cgggcggcac gctcgacaag ctcgaagcga
      241 taccgggcta ccgcgtccag ctcggcctcg acgagttccg cgcgatcctc gaaagcgtcg
      301 gctttgcgat gatggggcag acgagtacaa tcgcgcccgc cgaccggaag ctctacgcgc
      361 tgcgcgacgc gaccgagacg gtcgaagtcg tcccgctcat cgtcgcctcg atcatgtcga
      421 agaagctggc cgccggcctc gacgcgctcg tcctcgacgt gaagaccggc accggcgcct
      481 tcatgcacga ccgcgagcgc gccgcgcagc tcgcccgcgc gctcgtccag accggcaaca
      541 gcttcggcgt ccgcacggag gcgctcatca ccgacatgaa ccagccgctc ggccacgcgg
      601 tcggcaacgc gctcgaagcc aaagagtgcg tcgaaatcct gcgcggcgac gcgcacgaaa
      661 aggcgcggcc cgtcctcgac ctctcggtcg agctggccgc gcggatggtc aacatctcgg
      721 gcgtcgagcc ttcgctcgaa gaggcgcgcg agcgcgtcac gcaggcgctc gcgtcgggcg
      781 cggggctgga gctgttccgc cgcaacgtcg aggcgcaggg cggcgacccg cgcgtgtgcg
      841 acgctcccgc gcggctctcg gacttgacgc tcacggaggt cagagtagaa tccccgcgcc
      901 gagggctggt cgcgggcgtg gacgcggcgg aggtcggtcg cgccgttgcc gacatcggcg
      961 gcgggcgcgc gcgcatggag gacgagatcg accccgcggt cggcttcctc gcagaggcgc
     1021 gcgtcggcga cgaagtcaaa gaaggcgacg tgctcggcct gctctactgc cgcgacgccg
     1081 cgggcggcga ggaggccgcc gcgcgcatcc aagcggcgta cacggtctca agcgagtctc
     1141 ccgtcagagt gctgcggctc atcaaggaag tcatcacgcc atgaagtttc gagtctcact
     1201 ttcgctggct ctggtgtgcg cgctcgcgct ctcctcgggc gtcgcctgcc gctacgaggg
     1261 gcgcgtcgaa gggcaggcag acaaggtgca cgtcggcatc gtcttcgaca tcggcggcaa
     1321 ggacgaccgc tcgttcaacg ccgccgccta cgacggcgtg aagtgcgccg agcgcggcac
     1381 gaccgcgacc ggccagccct gcgagaagcc ctcgctcgac atcgtgctgc gcgacgtgga
     1441 gccgggcaac ccgacctcca tcgagccggc gatgcgcgcc ttcgccgaga gcggctacga
     1501 cctcgtcatc ggcgtcggct tcgcgcaggc gcccatcatg gagcgcgtgg ccaaggacta
     1561 cccgcgcatc aacttcgcca tcgtggacgg cgtcagccaa ctgccgaacg tcgcctcgct
     1621 catcttcaag gagcacgaag gctcgtacct cgcgggcatc atcgcggcgc gcgcctcgaa
     1681 gacgggcgtg ctcggcttcg tcggcggcat ggacatcccg ctcatccacc gcttcgagac
     1741 cggctacgag gagggcgcgc gctcggtcaa cccgaacgtc cgcgtcatcg acaactacgt
     1801 cggcgtcacc gacggcgcgt ggaacaaccc cggcaagggc aaggagctgg cgctcgcgca
     1861 gatcgacaaa ggagcggacg tgatcttcac cgccgccggc aactccggcc tcggcgtcgt
     1921 cgacgccgtc gagcagtacg gcagggacga gacgggccgc gcgcgcaagt tcgtcatcgg
     1981 cgtggactcg aaccagaaca tggtcaagcc cggcttcgtc ctgacgagca tggtcaagcg
     2041 agtcgataac gccgtctatt caatcgtcga ggacgtgaag aacaagcgct tcaaaggcgg
     2101 cgtccacgtc tacggcctcg acgccgacgg cgtcgcctac tcgctcgacc agtacaacaa
     2161 acccctcatc cccgcggaag tcttgcaaga ggccgaagcg gcgaagcaga aaatcatcgc
     2221 cggagaaatc aaggtgacgg acgcgatgaa cccgtaaagc agaggccgga ggtcagaggt
     2281 cggaggtcag ttgactgact tctactgacc tccgacctct gacctctaac ctctgagtta
     2341 tgctggaact gagagatatc accaagcgca tcggcgccgt gctcgccaac gacggcgtga
     2401 gcatcaaggt cgagaccggg acgatccacg ccatcgtcgg cgagaacggc gcgggcaagt
     2461 ccacggcgat gcgcatcgcc tacgggttct acacggcgga ctcgggcgag atcgtggtgg
     2521 aaggcgagcc gcgccagatc cagacgccgc acgacgccat ccggctcggc atcggcatgg
     2581 ttcatcagca cttcatgctg gtggacacga tgaccgtcgc ggagaacatc gtgctcggcg
     2641 cggagccggg cacggcggcg gcgatcgacc tcggcaaggc ggcgcgcgag attcgcaagc
     2701 tgtcggacga gttccgcctg tcggtcgatc ccggtgcgct ggtcgagacg ctgtcggtcg
     2761 gccagcagca gcgcgtcgag ctgctgaaag cgctctaccg gcgcgcgcgc atcctgattc
     2821 tcgacgagcc gaccgcggtg ctgacgccgc aggaggtcga ggagttcttc gccatcctgc
     2881 ggcgcatgcg cgagcaaggg aagacggtcg tcatcatcac gcacaagctc tcggaggtgc
     2941 tggccatctc ggacgaggtg acggtgatgc gcgacgggcg cgtggtgggc cgcgtgaaga
     3001 cgagcgagac ggacgcgggc gcgctggcgc gcatgatggt gggccgcgac gtgctgctcc
     3061 gcgtcgagaa gccggagccg aaagtcggcg gggctgtcct gaacgtcgaa cgcctgtcgg
     3121 tcgcggtgca cggcgtcgag cgcggcggcc tgcgcgatgt gtcgttcgcg gtgagggcgg
     3181 gcgagatcgt cggcatcgcg ggcgtcgagg gcaacgggca gaccgaactc atcgaggcgc
     3241 tcgcggggct ggtgccgcac gcgcaagtgt cgggcgccgt cgagttcgag gggcgcgaca
     3301 tcacgcgcac cggcgcgcgc cagcgccgcg aactcggcat cgcgcacatc cccgaagacc
     3361 ggcaccggcg gggccttctg ctcgacttcg acttgtcgga gaactcgatc ctcggcgacc
     3421 actaccgccc gcccgccgtg acgggcggcg tgctcctgaa caccggcggc atccgcgggc
     3481 gcgcgatgga agtcatccgc gacttcgacg tgcgcccgcc caaccccgac ctgccggcgc
     3541 gcgcgctctc cggcggcaac cagcagaagc tcatcatcgg gcgcgagttc gagctgcggc
     3601 ccaagctgct gctcgtctcg cagccgacgc gcggcgtgga catcggcgcc atcgaattca
     3661 tccatcgcaa gctcgtctcc ttgcgcgacg agggctgcgc cgtcctcctc gtctccgccg
     3721 aactcgaaga ggtcacgtcg ctcgccgacc gcctgctcat catctaccac gggcgcatcg
     3781 tcggcgaggt ggaccccgcg cgcgccacgc acgaggagat cggcctgatg atgaccggcg
     3841 gcgagaaaga agctgttagc cgttagcagt cagcttctag cttctgcgcc tcccgcgagc
     3901 ggcttgttcg gactcgatct tgggcgccgc caaacgctcg aagccaacag ctaacagcca
     3961 acggctaaca gctaacagcc ttcttatgaa acttctacgc gaactacttt tccccctcat
     4021 cgccgtggcc gcggcgttcg tggtcggcgg ggtggtggtg ctgctcgtgg gcgacaaccc
     4081 tctgaaggtg tacgggctgc tgctggggag cgcgctgtcg tggccggacg gcatcggcga
     4141 gacgctcttc aacgcgacgc cgctgatctt cacggggctg gcggtggcgg tggcgttccg
     4201 gtgcgggctg ttcaacatcg gggcggaggg gcagttgtac gtcgccgcgt ttttgaccgc
     4261 gtgggtctcg atcaagttcg gcgggacggt ggcggacgtg ttcggcaagc cggtcgatta
     4321 ctcgtgggcg agcctgccgg cggtcgtcct cgtgccgctg gccctgctga cggccatcgc
     4381 ggcgggcgcg gcgtgggggg cgataccggg cgtgctgaag gcgcggttcg gggcgcacga
     4441 agtcatcacg acgatcatgc tgaacttcat cggcgtcgcg ctcgtcagct atttcacgca
     4501 gtactactac aaggtgccgg gcgacccgat catgcagacg gtctccatcg gcgaggcggc
     4561 gcacatcccg cgcctctccg cgttcgtgcc gggcctgccg ccgcacctct ccgtcaacgt
     4621 cgccttcatc ttcgccatcc tcgcgtgcgt cttcgtctac ttctttttgt ggcggacgaa
     4681 gtggggctac gagattcgcg cgaccggcgc gaacccgacc gccgccgagt acggcggcat
     4741 ctcgaccggc aggcagatcg tcgtggcgat ggccgtgtcg ggcgcgctgg ccgggttggt
     4801 cgccatcaac gaggtgctcg gcttccgcta ccgctactac gacggcttct cggcgaacta
     4861 cgggttcacg gggatcgcgg tggcgctcct cgggcgcaac cacccggtcg gcgtctttct
     4921 ggcggcgctc ctgttcggcg cgctgatccg cggcggcatc ttcgtggacg ccttctccga
     4981 gcgcgtctcg aaggacatcg tcgaggtctt gcaggccgtc gtcatcctct tcgtcgccgc
     5041 cttcatgggc gccgaggcga tgctccgcag cgtgttcggc aggttcggac ttttgaagcg
     5101 gtcgaaacta tgagaacagt aggcagtaga cagtagacag cagctccgcc ctgcggctgc
     5161 tgacgctgac gcccgaacga ttcggaagag acggttggtc tactgcctac tgcctactgc
     5221 ctactgcttt tatggacatc ttcaatcttt cgctcatcta ctcgtcgatc cgcctgacga
     5281 cgccgctgct gctggccgcg ctgggcgggc tgtattcgga gcgcgcgggc gtcatcaaca
     5341 tcgcgctgga aggcttgatg ctggcgggcg cgttcacggc ggcggccatc acgcactacg
     5401 cgggcagccc gtgggtgggg ctgctggcgg gcgtcgtggc gggcgtcgtg gtggcggcca
     5461 tctacgcggt ggtgtgtatc cgcttcaagg ccgatcaggt ggtgagcggc accgccatca
     5521 acatcctgat ggtcggcgtg cccgcgatgt tgagcggggc gttcttcttc tcgtcgggtt
     5581 cgacgccgca gataccgaaa gagaatctgc tcccgaacac gcccatcgtc atcgcgttcc
     5641 tgctcgtgcc cgtgacgtgg tacgtgctct accggacgcc gttcgggctg cggctgcgcg
     5701 cggtcggcga gaaccccggc gcggcggacg cggcgggcgt gagcgtgacg aagttgcgct
     5761 actcggcggt gctgctctcg ggcgcgctcg caggcatcgc gggcgcgtac ctctccatcg
     5821 ggcagtcgtc gctgttcacg cgcaacatga cctcggggcg cggcttcatc gcgctcgcgg
     5881 cgctcatctt cggcaagtgg cggcccgtgc agacgatgct cgcgtgcctg ctcttcggct
     5941 tcacagaggc cgtcgccatc cagttgcagg gcgtccccat cggcggcgag gagataccga
     6001 accagttcat ccagatcatc ccctacgtcc tgaccatcgt cgtgctcgcc ggcttcatcg
     6061 gccagtcgcg cgcgccccgc gcgctcgggc agccctacca gaaggagcga tgaaacacac
     6121 cgtcgaagaa tgcaccgtcg tcctgcggca gctctccggc gagtggggcg tggacgaaga
     6181 gagcgtcgcc gtgggcagcc tcgaagagtt gtacgactac tgtctgaggc tgaaggagcc
     6241 gcggctgctc gaacggctcg tgctcgcggg gcgcgacgag cggggccgcg gtcgttccct
     6301 cactttcact tttcggtcga tcacggatcg gaataagacc caagcccccg acgagtccga
     6361 aggccgcggc tgaactcccg aaagcgggtg ggccgcaggc tgccgcaaac acgaacccga
     6421 gatgggcatt ctcatcaagc ctgaacacct gaagcgctac aaagacatcg cgtggctgct
     6481 gatgaagtac ggtcgcgccg acctggtgaa gaccgccggt ctcgaagcgg cggtcgaaga
     6541 gaccgaggcc gacggcgagg ggcacgcgct gcccgcgaag gccgaagagc tggccgcgga
     6601 cgtggagaag ctggggccga cctacatcaa gctcgcgcag cttctttcca cgcgcgccga
     6661 catgctgccg caagcctacc tcgacgcgct cgcgcgcctg caagacaagg tcgagccgtt
     6721 ctcgttcgcc gaggtcgagc agatcgtctc cgccgagctg ggcgtgcgga tgtcgaaggc
     6781 gttctccgat ttcgagtcgc gcccgattgc cgcggcctcg ctcgggcagg tgcaccgcgc
     6841 cagtttgcgc gacgggcgcg cggtcgtcgt caaggtgcag cgccccggca tccgcgagca
     6901 gatcacggag gacatggccg cgctcaacga gatcgccgcc ttcctcgacg gccacacaga
     6961 ggccgggcgc cgctacgagt tcacgcccat cctcgaagag ttccgcaaga cgatgctgcg
     7021 cgagctggac taccgcgtcg aggcgcacaa cctgctgacc ttcgccgaca acctgcgcga
     7081 gttcgaccgc atcgtcgtcc cgcagcccgt cgaggacttc accacctcgc gcgtcctgac
     7141 gatggactac atcaaggggc gcaagatcac cgacctctcg ccgctggcga agatagaact
     7201 cgacggttat caactcgccg agcatctctt ccaggcgtac ctccagcagc tcctcgtgga
     7261 cggcttcttc cacgccgacc cgcaccccgg caacgtcttc ctcaccggcg agaaccggat
     7321 gatcgcgctc ctcgacctcg gcatggtcgg gcgcctcgcg ccgcgtttcc aggagcatct
     7381 gctgcaactg ctgatcgccg tcagcgaggg gcgcggcgac gaggccgccg agatcaccgt
     7441 caagatcggc gagccgaagg aggagttcga cgccgagggc ttccgccgcc gcgtcgccga
     7501 gctggtcatc cagcaccaga cggccaagct cgaaaacatc aacgcgggcc gcgtcgtcct
     7561 ccagatcgcg cgcatctcgg gcgagcacaa cttccgcctc ccgcccgagt tcacgatgat
     7621 ctcgaagacg ctgctgaacc tcgaccaggt cgtccacacg ctcgacccga agttcgaccc
     7681 gaactactcg atccgcaact actcgacgac catcgtccag aagcgcgtgc gcaaggcgtt
     7741 ctccgcgggc gtcatgttcg gcaccgcgct cgagctgaag gagttcgtcg ataagctgcc
     7801 gggccgcgtc aaccagttga tggactcggt ggcgacgaag ggcctcgaaa tcaagatcga
     7861 cgccatcgac gaggtgcgcc tcatcgaagg catccagaag gtcgccaacc gcatcacgct
     7921 cggcctgctg ctggcgtcgc tcatcgtcgg cgcggcgctg ctgatgcgcg tcgaaacgac
     7981 cttccgcatt ttcggctacc ccggcatcgc catcatcttc ttcctgctgg cggcgggcgg
     8041 cgcccttgtg ttagctttca acatcctctt caacgacgag aaaaggaagt agcagtaggc
     8101 agtaggcagt aagcagtagg cagccacgaa cctgtggctt gaaactcgaa ggctcgaatc
     8161 cgaagttcgg agactcaacc ccgacaagaa atatgctttc gcctttaact gcctaccgcc
     8221 tactgcctac tgcctactgc ttatgagacc gatggaaggc atggtgccag aaacgttgta
     8281 cgagcgcgcg gagcacgcgg cgcgcttcat ccgcgcgcgg gcgaaggaag acccgcgcgt
     8341 cgcgctcgtg ctggggagcg gcctcggcgc gttcgccgac gagttggagg acgcggaggc
     8401 catcccgtac gaggagatac cgggcttcgc gcgcccgacc gtcgaggggc acgccgggcg
     8461 gctcgtcgtc ggcggggtcg ggggcgcgcc cgtcgccgcg atgcaggggc gcttccactc
     8521 ttacgagggc tactcgctcg aagaggtgac gtttcccgtc cgcgtgctgc gcctgctcgg
     8581 cgcacgcacc ctcgtcctga cgaacgcggc gggcgggctg aacaactctt acgcgcaagg
     8641 ctcgctcatc gtcatctccg accacctcaa cctgatgggc gtgaaccctt tgcgcggccc
     8701 gaacgacgag cgcttcggcc cgcgctttcc ggacatgacc tcggtctacg accacgagta
     8761 cgccgagacg gccatcaagg aggcgcgcga gatgaacctg gagctgcgcc gcggcatcta
     8821 cgcggcgctg cccggcccga gctacgagac gcccgccgag attcgcatgt tccgtctgct
     8881 cggcgccgac gccgtcggca tgagcaccgt ccccgaggcc atcgtcgcgc gccacatgtc
     8941 gatgcgcgtg ctcggcatct cctgcatcac gaacatggcc gccggcgtcc tcgaccgccc
     9001 catcgaccac gccgaagtga tcgagaccgg cgagcgcgtc cgcgagacct tcgcggaact
     9061 gctccggcgc gtgatcccga aactacagtg agcggtgagc agtaagcagt gagcagcatg
     9121 agaaagaaga aaattttact gcttactgct tactgctcac cgctcacttt cagagcgaag
     9181 cgatgataat aggcatctgc ggcgggacgg gttccgggaa gacgaccgtg gcgaaccgca
     9241 tcctcgaatc ggtgagcaag gaggaggtcg ggtttcttca gcaggactcg tactaccgcg
     9301 acatcgacca gttgccggtc gatctgcggc agcaggtcaa cttcgaccac ccggacgcca
     9361 tcgacaacga cctgctggtc gaacacctgc gggcgttgaa acgaggcgag gcggtggagc
     9421 tgccggtcta cgatttcaag aacaacgcgc gccgggccga gacgttgcac ctcgagccgc
     9481 ccgcgatcat catcatcgag ggcatcctga ttttcgccga cgagcggctg cgcgccgaga
     9541 tggacctgaa ggtcttcgtg gacacgcccg acgacatccg cttcatccgg cgcctcaggc
     9601 gcgacatcgc cgagcgcggg cgcaccgtcg agtcggtctg cgagcagtac gaggcgaccg
     9661 tgcgcccgat gcacatgcag ttcgtcgagc cttcgaagcg ccacgccgac gtgatcatcc
     9721 ccgaaggcgg gcacaacctc gtgagcatcg acttgatctc gggccgcgtc cgcgagcgca
     9781 tcgccgccga gcgccgccgc cgggccgcgc aaagggccgg cggcggcgcc gcggtcgcag
     9841 cggaacaggg agggtgagtg aagaccaaca ctgaacagga catcatcagg gccgccacag
     9901 aggcgcgcaa ctgcgcgctc gcgccctact cgaacttctg cgtcggcgtg gcggtcgagg
     9961 acgaggaggg gaacatcttc accggctgca acatcgagag cgcggcctac gagctgacga
    10021 tgtgcgccga gcgcgtcgcc atctggaagg cgctctccga aggcgcgcgc aagttccgcc
    10081 gcgtcgtcgt cgtcgccgac accgagcggc tgacgccgcc ctgcggcgcg tgccgccagc
    10141 tcgtctggga gttctgcggc gacgtgcccg tcgtgcttgc caacctgcgc ggcgacatcg
    10201 aggtcttgca gatggccgac cttttgccgc gccccttcga cgcgcagttc ctcgcggaga
    10261 ccaaagcggg cgagtgagag agggcgtgcg gctggcgatg aaaggcgaag ggaaagaaga
    10321 agaggcgcac ggcggcggca aggacggcgt ggagcgggag atcggcgaga tcacgcccgt
    10381 cgagcatttc ctcaaccgct acgtgggcta cgcgattcac ctcttcctct cgctcctcgc
    10441 tatcttcatc ctcgccgccg cgttgatagc gacgtaccag acgttggtct acgagttccc
    10501 gaagctcctg accccggcgg acgagtacaa atccctccag tccatcatcg ggcacatcct
    10561 gctcatcgcc atcgccgccg aactcggcct gctcctcctg ttccaccgca ccagcgccgc
    10621 cgtcgaggtc atcatcttcg tcatcgcgcg caagatcgtc acgcccgaca tctcctcgct
    10681 cgaactcctg ctcagcgtgg cggcgctcgc cgggctgctc gtcgtgcgct tctactacct
    10741 tcccggcaaa cccaaatgaa accacacagg ctcgcgtcca cctgtctcgc gctactgtcg
    10801 atatttatgc ccgtggtcgg cgcgtccgcg cagcgtgcgc gcggtgcgaa ccgcgtcgat
    10861 ttgatcgtgc gcggcgggac ggtcgtcacg atggacggcg agcgccgcgt catcgaagac
    10921 ggcgcggtcg ccgtcgagcg gggtcgcatc gtggcggtcg gcaggagcgc cgagatcgcg
    10981 cgcaggttca cctccgccga ggtcatcgac gcgcggggcc gcgccgtcat ccccggcctc
    11041 gtcaacggcc atactcacat cccgatgacg ctcttccgcg gcctcggcga cgacctcgac
    11101 ctcaacgact ggctgacgaa gtacatcttc ccggcggagg ccaagaacgt caccgaggag
    11161 ttcgtccgca cgggcacgca gctcggcctc gccgagatga tccgcggcgg gacgaccacc
    11221 tactgcgaca tgtactactt cgaggacgcc atcgccgacg agaccgcgcg cgccggggtg
    11281 cgcgccgtgc tcggcgagac ggtgatcgac ttccccgtcg ccgacaacaa gacgtggggc
    11341 gacgcgatgc gctacgtcgt gcgcttcgtc aacaagtgga agaaccaccc gctcgtcaca
    11401 cccgccgtcg cgccgcacgc gccctacacg gtctcggaag aacacctaaa ggatgtccgc
    11461 accttctccg acatgacggg cgcgcccatc gtcacccacg tcgcagagac gcgcaaagag
    11521 gtcgaggaca tcaccaagca gaaaggcgtg ccgcccgtcg aatacctcgc gcgaatcggc
    11581 ttcctcagcg agcgagtcat cgccgcgcac acggttcatc tcaacgagcg cgagatcggt
    11641 ctactgaaag aggcgggcgt cggctcggtg cacaacccgg agtcgaacat gaagctggcg
    11701 tcgggcgtcg cccctgtgcc gcggatgctc agggaaggga tgcggctcgg actcgggacg
    11761 gacggcgcgg cgtcgaacaa cgacctcagc atgtgggagg cgatggacgc ggcggccaag
    11821 ctgcacaagc tcacggcgaa cgacccgaag gtcgtcaccg ccgaagaggc tttgatgatg
    11881 gcgaccatcg gcggcgcgcg cgcgctgcat atggagaagg agatcggctc gctcgaaacg
    11941 gggaagcggg ccgacctcgt cgtcgttgac ttggacgcgc tgcaccagac gccgcgctac
    12001 aacatctact cgcacctcgt ctacgcgacg aaggcttcgg acgtgcgcac ggtcgtcatc
    12061 gaggggcgcg tcgtgatgcg agaccggcgt ctgctgacgc tcgacgaggc gctcatcaag
    12121 cgcaaggcgc gcgccatccg cgagcgcgtc tcgcgcagcc tcgcggcttt ttcgcccgga
    12181 aacggcggga aaacgccgcc gccctcgcgg gccgccgccc gccgggttga tagaaaagac
    12241 gaataaggcc ataatgcgcc gtaacaaata cgtgataccc ggaggcgagc gtcgttggct
    12301 cgcttccgag tttgtggagt gattgatatt gaagggaacc agttttttcg acaacgcgcg
    12361 cctgccccgc attcttcttt acagcctgtt catgactctc accgttgcgg taacgggcgc
    12421 gcaggcgcag acgcagacaa caacggaacc tgccgtcacg tccaacgcgg cgacgccgac
    12481 gcccgcgccc acgcctacgg agacggaagc ggtgcagcgc cgcatcgcgc gcgcgcgcgc
    12541 gctggcggcg gtcggcagac tggcggcggc ggcgggcgag cttgaatccc tgcgggccgc
    12601 cagcacggac gagtcggtgc gcgacgtggc gcgcctgctg ctgatgtgga ttttcgtcga
    12661 gatgcccgac tacgcgcgcg ccaacgccct gctcgatgag acctacaagg agcgcggcac
    12721 ggaggcggcg cgcgtctcct accacgcgct cgcgggccag acgatcaacg gcgtccgcgc
    12781 ccacctcgaa cgctaccgca ccttcggcat caacgtcgcc gacgcggact tgcccgccga
    12841 ggccgtgacc gacctcgacc agttgcgcgg gctgttggag cggctggtcg agcacgcgaa
    12901 gacgatgcgc ggcgaggagg acgcgaagtc caccgccgcg cgaagcacgg agacgacggc
    12961 gctcctcgaa gacgccgcga gcgtccgcct gcgcctcgcg cgcaacgacc aggagcgcac
    13021 gcgctggcag ggcgaagtct ccgaagcgcg ccagcggctc gtcgcctccg agacgcgcat
    13081 cgccagcatc agcagcgtcc ccttcagccg ccccaccccc gcgcccccga cgaccacggc
    13141 accgtcgcct tccgtctcgg acgcgcccgg caggggcgcc cccgcgaaga ccgccgaccg
    13201 gcagaagacc gccgcgcccg aacgcgccgc caacggcagc gctaacaact cgaccgcgtc
    13261 aaacgcgccc gccgcctcgc agaaccccgg acaggcgtcc gccactcccg ccgcgggcga
    13321 gcggcagccc gtcgccatcg gctctctgca caccgtcgcg cggcagaagt ccgcgccctc
    13381 ctacccctcg attgcgcgcg cggcccgcgt cacgggcgtc gtcacggtct tcctcgtcgt
    13441 caacgagaag ggcgaggtcg aatccgtcac ccgcgccgac ggcccgcagc agttgcagaa
    13501 cgccgcctcg gacgccgccc gccgctggaa gttccacccc accgtcatcg acggccagcc
    13561 cgtgcgcgtc accggctaca tcagcttcaa cttcgcgctg taagaccctc cgtcctgcga
    13621 ggaaaccgtt catgacctac gcgctgtgga tcgtgcaggc gctgctcgcg ctcctgttcc
    13681 tgttcgccgg agggacgaag ctggtcatac cgcccgaggt gctggcgtcg atggggtcgc
    13741 cgaaccagat tccgctgccg ggatggttcg tgcgcttcat cggcgtggcc gaggtgctcg
    13801 gcgcgctcgg actgatcctg cccggcctgc tgcgtatcaa gccgtggctg acgccgctgg
    13861 ccgccgtcgg gctgttgatt atcctgatcg gcgcgacggc gctctcgttc gccgccgacg
    13921 gaatcgcggc ggcagtggtt cccctggtgg tggggctgct cgcggcgttc gtcgcctacg
    13981 ggcgctggcg gctcgcgccg cacgcgggcc gcggcgagaa gtgacgcgga agtgttcggg
    14041 ttgacgaagg cgtgcgccgc cgcggtcgat gccgacgacg tacggcccct cagtgcgtcc
    14101 cgcggcgcgg cgttttggcg gaagggctga tgcggacgaa ctcgggcgcc ggtcgtgcgg
    14161 gctgtttcgc ttcgccgcag gcgccgcaac cggtcgagca ggcgggcgcg ccctgccgct
    14221 tcgagagacg gaaagagcgc aggcgcgaga gagcgcgccg cgcgacgtag gccagcgcga
    14281 gcatgtaggc gaccatgaag agcggcca
//
LOCUS       DBNGOH010000294         1824 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4444060, whole genome shotgun sequence.
ACCESSION   DBNGOH010000294 DBNGOH010000000
VERSION     DBNGOH010000294.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1824)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1824)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1824
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4444060"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..690
                     /locus_tag="AC159P_05695"
     CDS             <1..690
                     /locus_tag="AC159P_05695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694981.1"
                     /translation="AAVETLKTTGRRRLLHAEDRAPANTTFIKPPPDANDMKNAPRPL
                     RLLLLAALLTCAAPALAAGAAATAQKREHLTTEEIEFIRDAQRLDLRTGVFIKAAERR
                     LLMIADPASKQLGKDAEKWGELKGTRRQLYDDVYRILDEAVVNIDDTAQRDPKSALLN
                     KSLSKLAEAARRFLPQLAPLLNSAQDDGERKTLAEVIEKAEEIIEAAKRHDVDAVIEK
                     EKSGKKGDKEN"
     gene            861..1439
                     /locus_tag="AC159P_05700"
     CDS             861..1439
                     /locus_tag="AC159P_05700"
                     /inference="COORDINATES: protein motif:HMM:NF039185.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HIR3694982.1"
                     /translation="MDNRFYPGVEVDAPQLLQELLRLFDGDDAFEVQTMQVASASVLQ
                     ARKSSTLRDLTGLSAALTIKVTPEHGGTRVEIGMQKWFDKAAVAAVAMFLSLGLLVAL
                     PALGAYWQHKLTEDAWKIIEAHIARKAGGYVPPLPGRCGTCGAVVVSGAAFCSTCGAD
                     LRVRRSTCASCGATQPDTSARYCNRCGKPLTG"
     gene            complement(1538..>1824)
                     /locus_tag="AC159P_05705"
     CDS             complement(1538..>1824)
                     /locus_tag="AC159P_05705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694983.1"
                     /translation="QVENLPHKRTKEVKNEQSYRERECIHRAGGAEGNYYKGDIYVKH
                     LMRLPSARAHSFAGRVTSFFWADAAQIVVWLCDDCASELGLYVSETNLVR"
BASE COUNT          342 a          650 c          549 g          283 t
ORIGIN      
        1 gccgcagtcg aaaccctgaa gacgacgggc cgccgccgcc ttcttcatgc agaagaccgc
       61 gcgcccgcca acacgacttt catcaagccc ccgcccgacg ccaacgacat gaagaacgca
      121 ccacgccccc tccgccttct gttactcgcc gcgctgctca cctgcgccgc ccccgcgctc
      181 gcggcgggcg ccgctgccac agcgcagaag cgcgagcacc tgacgaccga agagatcgag
      241 ttcatccgcg acgcgcagag gctcgacctg cgcaccggcg tcttcatcaa ggccgccgag
      301 cgccgcctgc tgatgatcgc cgaccccgcc tccaaacagc tcgggaagga cgcggagaag
      361 tggggcgagc tgaagggcac gcggcggcag ctctacgacg acgtgtaccg catcctcgac
      421 gaggccgtcg tcaacatcga cgacaccgcc cagcgcgacc ccaaaagcgc gctcctcaac
      481 aagtcgctct ccaaactcgc cgaagccgcg cggcgcttcc tcccgcagct cgcgccgctc
      541 ctcaactccg cccaggacga cggcgagcgc aagaccctcg cggaggtgat cgagaaggcc
      601 gaggagatca tcgaggccgc caaacgccac gacgttgacg ccgtgatcga gaaggaaaag
      661 tccggcaaga aaggcgacaa ggagaactga ccgaaagggg aaaaggttaa aaggggaaaa
      721 ggcgaaacgg gaagaaccga cttcttcttc ccgtttcgcc ttttcccctt ttaacctttt
      781 ccccgtctcc cttttgaacc tcggcttgtg ttttggcgta gaataggtcg caggacgcga
      841 aaggaatcat cgggaagaac atggacaaca gattctaccc cggcgtcgag gttgacgcgc
      901 cgcaactgct acaggagctg ctccgcctgt tcgacggcga cgacgccttc gaggtgcaga
      961 ccatgcaggt cgcctcggcc tccgtccttc aggcgcgcaa gtcttcgacc ctgcgcgacc
     1021 tgacgggcct ctccgccgcg ctcaccatca aggtcacgcc cgagcacggc ggcacccgcg
     1081 tcgagatcgg catgcagaag tggttcgaca aggcggcggt ggccgccgtc gccatgttcc
     1141 tctcgctcgg cctgctcgtc gcgctgcccg cgctcggcgc ctactggcag cacaagctca
     1201 ccgaggacgc ttggaagatc atcgaggcgc acatcgcgcg caaggcgggc ggctacgtcc
     1261 cgccgctgcc gggccgctgc gggacgtgcg gcgcggtggt cgtctccggc gcggccttct
     1321 gctcgacctg cggcgcagac ctgcgcgtcc gccgctccac ctgcgcctcc tgcggcgcca
     1381 cgcagcccga cacctccgca cgctactgca accgctgcgg caaaccactc acgggctgaa
     1441 agtagacggg gagtaaaggg agacggggtg acggggagaa agtgaagggc gcagtttccc
     1501 cccgtcaccc cgtctccctt tctccccttc gcggcaatca cctgacgagg ttcgtctcgc
     1561 tcacgtagag gccgagttcc gaggcgcagt cgtcgcacag ccagacgacg atctgcgcgg
     1621 cgtcggccca gaagaacgag gtgacccggc ccgcgaagct gtgcgcgcgc gcgctcggca
     1681 gccgcatcag gtgtttgacg tagatgtcgc ccttgtagta attgccttcg gccccgcccg
     1741 cgcgatggat acactcgcgc tcacggtacg actgctcgtt cttcacttcc ttcgtcctct
     1801 tgtgtggcag attttcaact tgga
//
LOCUS       DBNGOH010000295         4696 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2794347, whole genome shotgun sequence.
ACCESSION   DBNGOH010000295 DBNGOH010000000
VERSION     DBNGOH010000295.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4696)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4696)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4696
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2794347"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2195
                     /locus_tag="AC159P_05710"
     CDS             <1..2195
                     /locus_tag="AC159P_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0097054 - L-glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HIR3694984.1"
                     /translation="VGKVARGEQPYENFVARLREIEETKPVAIRQLLDIRDSLGRDAN
                     PAHGGEVDTRAGRHDAPFYFAAMSFGSQGETSFRAYAEAAKRLDIVCINGEGGEIPDM
                     LGRYREHRGQQVASGRFGVNARFLNSCNLIEIKIGQGAKPGEGGLLPGFKVTARIAET
                     RHTPAGIDLISPSNNHDIYSIEDLAQVIEELKTANTHARISVKIPAVPYLGPIATGIA
                     KARADIIAISGYDGGTGAARKHSLRHTGLPVEIGLREAHRALVRSGLRQNVELWADGG
                     VKSGRDVVKLMLLGADRVGFGTMAMAAIGCTSCRECNTGTCHVGITSQLKDASEALAA
                     GQKHFTPREDERAVEHLVRFFTEVREEIKRETARAGLKATRELVGRVELLEQVRCTDR
                     LDLAWLLQPGEPCRLCSSAQEPHVGRARRSLTSLTRVISASLISLAEAGKEVVYFDDA
                     YASSGDRALGTHLAGKLVRTRFGDGNGNGNGHDTGSLINDNGNNGGHARRIRRAVLNF
                     LEGSVPGNGLGAFNADGVDIIVHGGVQDGAAKGASGGRIVILKGVNHDGVAVDGAVGK
                     CFAYGAQAGLFIVQGDADSRAGIRLSGADLVIGGRLRGGVRDSLGNIAARANIKGFAF
                     EYMTSGRGLVLGDPGPWLCSGMTGGVVYLLIDEELGLTTEALRRRLATGALVSLEPVG
                     ESDEANLGELLCHYRSALAEGQQHAEAEQVAALARDWRQRFVKAVPKK"
     gene            2312..4093
                     /gene="ilvB"
                     /locus_tag="AC159P_05715"
     CDS             2312..4093
                     /gene="ilvB"
                     /locus_tag="AC159P_05715"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015725197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HIR3694985.1"
                     /translation="MIAQQLVNPTKVASPLSTPTPAVETLPRGAEILIEALLHEGVDS
                     VFGYPGGAVLHIYDELWRYRERITHYLVRHEQGAVHMAEGYARSSGKVGVALVTSGPG
                     ATNAVTGIANAYMDSTPIVVITGQVPLPLIGTDAFQEVDTVGITRPCVKHNYLVRDAR
                     DLAGVVHEAFYLARTGRPGPVVIDIPKDVSAARCSYSRLDHIAFPCTEKRAAPDAQAI
                     SSATDAILESERPVLYVGGGIVNSGASEALLAFADGLQLPVTPTLMGLGGFPSAHPLC
                     LNMLGMHGTYAANMAVAESDLLIAVGVRFDDRVTGKLATFAPHARVIHVEIDPANIGK
                     NVLPTLSITADAREALAALREETVARGDAAVEESIARRNAWWERIRAWQREQPLRFSG
                     SAEQIKPQTVIRELHRLTKGEAIITTDVGQHQMWVAQFYPFTRSRQLITSGGLGTMGF
                     GLPAAIGAQLAFRDRLVVAVVGDGGFQMTNQELATAVQYNLPVKILIMNNGYLGMVRQ
                     WQEMFYDRAYSEVDISVAPDFVKLAEAYGAGGFRAATPEELPSVLEAALAHPGVAVID
                     VLVSKEENVFPIVPAGSNSRDMIVQQP"
     gene            4208..>4696
                     /gene="ilvN"
                     /locus_tag="AC159P_05720"
     CDS             4208..>4696
                     /gene="ilvN"
                     /locus_tag="AC159P_05720"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013885901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HIR3694986.1"
                     /translation="MRHTLSIIVADQPGELSRIVGLFSARGFNIETISVGGTLDPEWS
                     RVTVVTSGDERTIEQIVKQCERLARVREVRDVTSQPHIEREMALIDVTASPGAERQEV
                     LSLVNVFRAKVVDISHTRMVIEASGNREKVEAFVEMLRPLGINDITRTGYIAIGRLSA
                     TEE"
BASE COUNT          778 a         1679 c         1526 g          713 t
ORIGIN      
        1 tcgtcggaaa ggtcgcgcgc ggcgagcagc cgtacgagaa tttcgtcgcg cgcctgcgcg
       61 agatcgaaga gacgaagccc gtcgccatcc ggcaactgct cgacatccgc gacagtctcg
      121 ggcgcgacgc gaaccccgcg cacggcggcg aagtggacac gcgcgccggt cggcacgacg
      181 cccctttcta cttcgccgcg atgagcttcg gctcgcaggg cgagacctct ttccgcgcct
      241 acgccgaggc ggccaaacgt ttggacatcg tctgcatcaa cggcgagggc ggcgagatac
      301 cggacatgct cggtcgctac cgcgagcacc gcggccagca ggtcgcttcg gggcgcttcg
      361 gggtcaacgc gcgcttcctc aactcctgca accttatcga aatcaagatc gggcagggcg
      421 ccaagcccgg cgagggcggc ctgctgcccg gcttcaaagt caccgcgcgc atcgccgaga
      481 cgcgccacac gcccgccggg attgatttaa tctcgccctc gaacaaccac gacatctact
      541 ccatcgaaga cctcgcgcag gtcatcgaag agctgaagac cgcgaacacg cacgcgcgca
      601 tctcggtcaa gattcccgcc gtcccttacc tcggcccgat tgcgaccggc atcgccaaag
      661 cgcgcgcaga catcatcgcc atcagcggct acgacggcgg caccggcgcc gcgcgcaagc
      721 acagcttgcg acacacgggc ctgcccgtcg aaatcggctt gcgcgaggcg caccgcgcgc
      781 tcgtccgctc gggcctccgc cagaacgtcg agctgtgggc cgacggcggc gtcaagtcgg
      841 gccgcgacgt ggtgaagctg atgctcctcg gcgcggatcg cgtcggcttc gggacgatgg
      901 cgatggcggc catcggctgc accagttgcc gcgagtgcaa cacgggcacg tgccacgtcg
      961 gcatcacgag tcagctcaaa gacgcttcgg aggcgctcgc cgccgggcag aagcacttca
     1021 cgccgcgcga ggacgagcgc gcggtcgaac acctcgttcg cttcttcacg gaagtcaggg
     1081 aagagatcaa gcgcgagacg gcgcgcgccg ggctgaaggc gacgcgcgaa ctggtgggcc
     1141 gcgtcgagct gctcgaacag gtgcgctgca cagaccgtct cgacctcgcg tggctgctgc
     1201 aacccggcga gccgtgccgg ctctgctcct cggcacagga gccgcacgtc ggtcgcgcgc
     1261 gccgctcgct cacctcgctg acgcgcgtca tctcggcctc gctcatcagc ctcgccgaag
     1321 cgggcaagga ggtcgtctac ttcgacgacg cctacgcttc gagcggagac cgcgcgctcg
     1381 gcacgcacct cgcgggcaag ctcgtccgca cgcgcttcgg cgacggcaac ggcaacggca
     1441 acggtcacga caccggctct ctcatcaacg acaacggcaa caatggcggt cacgcgcggc
     1501 gcatccgtcg cgcggtcttg aactttctgg aaggctccgt gccgggcaac ggcctcggcg
     1561 cgttcaacgc cgacggcgtg gacatcatcg tccacggcgg cgtgcaggac ggcgcggcca
     1621 aaggggcttc gggcggtcgc atcgtcatcc tcaagggcgt caaccacgac ggcgtcgcgg
     1681 tggacggcgc cgtcggcaaa tgcttcgcct acggcgcgca ggccggcctc ttcatcgtgc
     1741 agggcgacgc cgactctcgc gcgggcattc gtctctcggg cgccgacctc gtcatcggcg
     1801 gtcgtttgcg cggcggggtg cgcgactcgt taggcaacat cgccgcgcgc gccaacatca
     1861 agggcttcgc cttcgagtac atgacctcgg ggcgcggcct cgtcttgggc gatcccggcc
     1921 cgtggctctg ctccggcatg acgggcggcg tcgtctatct actcatcgac gaagaactcg
     1981 gcctgacgac cgaagcgctc cgccgccgcc ttgccaccgg cgcgctggtt agcctcgaac
     2041 ccgtcggcga atccgacgaa gcgaacctcg gcgagctgct ctgccactac cgcagcgcgc
     2101 tcgccgaagg ccagcagcac gcggaggccg aacaggtcgc cgcgctcgcg cgcgactggc
     2161 gacaacgctt cgtcaaggcc gtcccgaaga agtagtggtc agtggccggt ggtcagtggc
     2221 tccgcgaagg ccgttgccgc cggcgtgccg acgattcgct gccgcaagtt cttccaccgg
     2281 ccactgacca ctgaccaccg gccacttatt tatgatcgca caacagctcg tcaatccgac
     2341 caaagtcgcc tcgcccctgt ccactcccac gccggcggtc gagacgctgc cgcgcggcgc
     2401 cgagattctg atcgaggcgc tgctgcacga gggcgtcgat tcggtcttcg gctatccggg
     2461 cggcgcggtg ctccacatct acgacgagct gtggcgctac cgcgagcgca tcacgcacta
     2521 cctcgtccgc cacgagcagg gcgcggtgca catggccgag ggctacgcgc gctcgtcggg
     2581 caaggtcggc gtggcgctcg tcacgtccgg cccaggcgcg accaacgccg tcaccggcat
     2641 cgccaacgcc tacatggact cgacgcccat cgtcgtcatc acgggccagg tgccgctccc
     2701 gctcatcggc accgacgcgt ttcaggaggt ggacacggtc ggcatcacgc ggccctgcgt
     2761 caagcacaac tatctggtgc gcgacgcgcg cgatctggcg ggcgtcgtgc acgaggcgtt
     2821 ctatctcgcg cgcacgggcc gccccggccc cgtcgtcatc gacatcccga aggacgtgtc
     2881 ggcggcgcgc tgctcttact cgcggctcga ccacatcgcc ttcccgtgca cggagaagag
     2941 agccgcgccg gacgcgcagg ccatcagcag cgcgacggac gcgattctcg aatccgagcg
     3001 cccggtgctc tacgtcggcg gcggcatcgt caactcgggc gcgagcgagg cgctgctcgc
     3061 gttcgccgat gggctgcaac tgcccgtcac gccgacgctg atgggactcg gcggcttccc
     3121 ctcggcgcac ccgctctgcc tcaacatgct ggggatgcac ggcacgtacg ccgccaacat
     3181 ggcggtcgcc gagagcgact tgctaatcgc cgtcggcgtc cgcttcgacg accgcgtgac
     3241 cggcaagctc gccaccttcg cgccgcacgc gcgcgtcatc cacgtcgaga tcgacccggc
     3301 caacatcggc aagaacgtgc tccccacgct ctccatcacg gcggacgcgc gcgaggcgct
     3361 cgccgctctg cgcgaagaga cggtcgcgcg cggcgacgcc gcggtcgaag agagcatcgc
     3421 gcgccgcaac gcctggtggg agcgcatccg cgcgtggcag cgcgagcagc cccttcgctt
     3481 cagcggctcg gcggaacaga tcaagccgca gaccgtcatc cgcgagctgc accgcctgac
     3541 caaaggcgag gccatcatca cgaccgacgt gggccagcac cagatgtggg tcgcgcagtt
     3601 ctaccccttc acgcgctcgc gccagctcat cacgagcggc ggcctcggca cgatgggctt
     3661 cggcctgccg gcggccatcg gcgcgcagct cgccttccgc gaccgccttg tcgtcgccgt
     3721 ggtcggcgac ggcggcttcc agatgaccaa tcaggagctg gcgaccgccg tgcagtacaa
     3781 cctgccggtc aagattctga tcatgaacaa cggctacctc ggcatggtgc ggcagtggca
     3841 ggagatgttc tacgaccgcg cctactcgga ggtggacatc tcggtcgcgc ccgacttcgt
     3901 caagctcgcc gaggcctacg gcgccggggg cttccgcgcc gcgacgcccg aagaactccc
     3961 ttcggtgctc gaagccgccc tcgcgcatcc gggcgtcgcc gtcatcgacg tgctcgtctc
     4021 gaaggaggag aacgtcttcc ccatcgtccc cgccgggtcg aactcgcgcg acatgatcgt
     4081 ccaacagccg tgaaccgtga accgtaaacc gtgataagtg aaaacagctt tcttcttatc
     4141 acggcttacg gttcactcgt cgcggctctt acttgtcacg gttgacggtt cacggttcac
     4201 ggtttttatg cgtcacacac tctccatcat cgtcgccgat cagccgggcg agttgtcgcg
     4261 gatcgtcggg ctgttcagcg cgcggggctt caacatcgag acgatctcgg tcggcgggac
     4321 gctcgacccg gagtggtcgc gcgtgacggt cgtgacgagc ggcgacgagc gcaccatcga
     4381 gcagatcgtc aagcagtgcg agcgcctcgc gcgcgtgcgc gaggtgcgcg acgtgacgag
     4441 ccagccgcac atcgagcgcg agatggcgct catcgacgtg acggcctcgc ccggcgccga
     4501 gcggcaggag gtcttgagtc tggtcaacgt tttccgcgcg aaggtcgtgg acatttcgca
     4561 cacgcgcatg gtcatcgagg cgtcgggcaa ccgcgagaag gtcgaagcct tcgtcgagat
     4621 gctccgcccc ctcggcatca acgacatcac acgcaccggc tacatcgcca tcgggcggct
     4681 gtcggcgacg gaagaa
//
LOCUS       DBNGOH010000296         1843 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4444227, whole genome shotgun sequence.
ACCESSION   DBNGOH010000296 DBNGOH010000000
VERSION     DBNGOH010000296.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1843)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1843)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1843
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4444227"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            453..1139
                     /locus_tag="AC159P_05725"
     CDS             453..1139
                     /locus_tag="AC159P_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR3694987.1"
                     /translation="MSGVLAVTAEVPGGWRQLAAAVETEETSGAAESPERSFDYALAQ
                     RAASGDLEAFEEIYRLYHRRVYSVCLRMTCNVSEAEDLTQEVFIHQLRKLGGFRGESA
                     LGTWLHRLTVNQVLMHFRRHRARREQPRDEEHGEQTVALGAEHEARPPVIDHIALSDA
                     LARLPEGYRTIFILHDVEGYEHEEVARLLGCAPGTSKSQLHKARKRLRELLGARSHKS
                     LITHTRRPRT"
     gene            1136..1381
                     /locus_tag="AC159P_05730"
     CDS             1136..1381
                     /locus_tag="AC159P_05730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694988.1"
                     /translation="MRPRLPPTPAASIYTNRHLCKTTRARDTSRPPLLRPRTGRGENA
                     GHLFWLTKLNQFGGNYVDAEMVKAFIVCVEYRQRFGQ"
     gene            1564..>1843
                     /locus_tag="AC159P_05735"
     CDS             1564..>1843
                     /locus_tag="AC159P_05735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694989.1"
                     /translation="MKTAYDEPRDEPQLRPLFDEIGGILDSRFYREDARTGLFKDLQP
                     EIIADDIPAFIDLIDVVAQNLVGLLKQLRESQAGPAPSPPPPAGEGGAG"
BASE COUNT          323 a          652 c          619 g          249 t
ORIGIN      
        1 ccacgtcgag ctggacgtgg cgcgggcgcg gatgatgtcg cagatttacg cgctcctgac
       61 cgccgagcag aaagtcacgc tcgccgagcg ccgccagcag ttcgagcagc gccgccgcgc
      121 gcacgagccg aagacgggcg aagcgccgag cggcaaatag ccgaccgcgt tccgctatcg
      181 acggctgacc gcgcgcgttt tctcgggggg agtcggcgcg gcgcgggtca gggcggcccg
      241 cgccgacggg acaccgctcc ccccggtggc ggaggagacg agggggacat gaccgggagg
      301 cacgcggggg cggcccgtcg cgcgcgaggg cgcgggccac ggtgcgaccg cgggccacag
      361 tccccctcgt cccgtcacct cgaaccgaac ccttgaacag gaacgagggc cgcgcgcggt
      421 gcggcgcgtg gccgggagaa aggcggtcgg cgatgtccgg tgtgttggcg gtgacggcgg
      481 aggttccggg cgggtggcgg cagctcgccg ccgccgtcga gacggaagag acgagcggcg
      541 ccgccgagtc gcccgagcgt tcgttcgact acgcgctcgc gcagcgcgcg gcctcgggcg
      601 acctcgaagc gttcgaggag atttacaggc tctaccaccg ccgcgtctac tcggtctgcc
      661 tgcggatgac gtgcaacgtc tcggaggccg aagacctgac gcaggaggtc ttcatccacc
      721 agctgcgcaa gctcggcggc ttccgcggcg agtcggcgct cggcacgtgg ctccaccgcc
      781 tgacggtcaa tcaggtcttg atgcacttcc gccgccaccg cgcgcgccgc gagcagccgc
      841 gcgacgaaga gcacggcgaa cagactgtcg cgctcggcgc cgagcacgaa gcgcgcccgc
      901 ccgtcatcga ccacatcgcg ctctcggacg cgctcgcgcg actgcccgaa ggctaccgca
      961 cgatcttcat cctgcacgac gtggaaggct acgagcacga agaggtcgcg cgcctgctgg
     1021 gctgcgcgcc cggcacctcg aagtcgcaac tgcacaaggc gcgcaagcgc ctgcgcgagc
     1081 tgctgggcgc gcgctcgcac aaaagcctca tcacccacac ccgccggccg cggacttgag
     1141 acctcgcctc ccgccgactc cggcagcctc gatttacacc aaccgacacc tgtgcaaaac
     1201 tacgcgggcg cgcgacacct cccgtccgcc gctcctccgt cctcgcacgg gccgtgggga
     1261 gaacgccggc cacctgttct ggctgacgaa gctcaaccag ttcggcggca attacgttga
     1321 cgcggagatg gtcaaagcct tcatcgtctg cgtcgaatac cgccaacgct tcggccagta
     1381 aatcagcttc aatccatagt cgtcgggcca aggcactgcg gagtttccga aagcaggagg
     1441 acggcgtatg gccttcgtac ccgggtacga aaacgacatc ttcatcagct atgcgcacat
     1501 cgacaacgag ccggtcatta cgggcaagcc cggctggccg gcagcagccg ggtcatcaag
     1561 gtggtgaaga ccgcttacga tgagccgcgg gacgaaccgc agctccgacc gctgttcgac
     1621 gagatcgggg gcatcctcga ttccaggttc taccgcgagg acgcgcggac ggggctgttc
     1681 aaagacttgc agcccgaaat catcgccgac gacatccccg ccttcatcga cctcatcgac
     1741 gtcgtcgccc aaaacctggt cgggcttctc aaacaacttc gcgaatcgca ggccggcccg
     1801 gcgccgtcac cgccgccgcc tgccggagag ggcggcgccg gcg
//
LOCUS       DBNGOH010000297         4417 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2794525, whole genome shotgun sequence.
ACCESSION   DBNGOH010000297 DBNGOH010000000
VERSION     DBNGOH010000297.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4417)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4417)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4417
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2794525"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..878
                     /locus_tag="AC159P_05740"
     CDS             <1..878
                     /locus_tag="AC159P_05740"
                     /inference="COORDINATES: protein motif:HMM:NF013279.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HIR3694990.1"
                     /translation="VIVLFPGIGLKINGQRAWIGVPGLGQFQPSEFMKIPTVLMLARY
                     FGRPRQSTLRLKEVVAGGLILALPVALILLEPDAGQALTYIPLLAVVLFLSSVRMWLV
                     VAAIALATVAVPAAYVVGVQTGAIKGYQQERINVILDPENADRRGFGYHTWQSIVTVG
                     KGGLKGSGTAGDASQSRLKFLPEPHTDFIFAVTAENTGFVGSILLLLAYAVLLTKLIH
                     GARRAPDRTGMLVIMAIVGGLAFQILINVGMALGILPVIGVPLPLMSAGLSAILATSV
                     AIGFAISIRLRRYVN"
     gene            904..2031
                     /locus_tag="AC159P_05745"
     CDS             904..2031
                     /locus_tag="AC159P_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR3694991.1"
                     /translation="MNRPGTSRRGFLRSALSAGALVAGSGGWPARAFAAESRAERRFA
                     PVRVSRERLIRTVVGLRPYRAEGFVVKAERVGEKLVVHNYGHGGAGVTLSWGTAKLAA
                     DEVRGVGGQERFAVVGCGVSGLSLARVLQRRGGAVTIYARELPPETTSNVAGALWLPT
                     SSYNPQRVSAAFPEQFRAACEVSNRAFQLMVGAEYGVRWMETYTVHTTEEEAARELLG
                     GARLYPETKLHRDAKRYFGAPLVRQFSTMLIEPSVYLNALLRDFHAAGGRVVVKELRG
                     REELAALAEPVVFNCAGLGARALFADEGLVPVRGQLEVLLPQPEVDYCLLNGSYYMFP
                     RRDGIILGGTFERDNWSLEPNAETTALILDANAEAMKRVRR"
     gene            2151..2723
                     /locus_tag="AC159P_05750"
     CDS             2151..2723
                     /locus_tag="AC159P_05750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HIR3694992.1"
                     /translation="MRLMILAVALFVAAGLTTFPAADKASARRHAQAAAGAVTPCDAQ
                     AVVNDPDPQGMNVRSGPGGTFKVIGNLPNADVNGISVHITGAQGEWVRIDRADEQGGE
                     PEDRVFFKGLGWVYAPLLTVDGVGGIAGGTKLYKEPSAKSRVVARMPVDVGGATVHGC
                     KGKWLYVEHKKVRGWAAPGTTCSNALTNCN"
     gene            2729..2950
                     /locus_tag="AC159P_05755"
     CDS             2729..2950
                     /locus_tag="AC159P_05755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3694993.1"
                     /translation="MKGLRRHTHEGRRRVVEGLAPLVRGEYRDLPKLRETAERVFEEF
                     EGIFEAPGVELYDESVDPNDDPPPRVRSG"
     gene            2956..3588
                     /locus_tag="AC159P_05760"
     CDS             2956..3588
                     /locus_tag="AC159P_05760"
                     /inference="COORDINATES: protein motif:HMM:NF013933.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HIR3694994.1"
                     /translation="MSLEVILVFAATEFVLSLTPGPAVLVVVSQGMRHGFAASARGAA
                     GILAGNAVYFALSALGLGALLLASATLFAVLKWAGVAYLVFVGLRMLLAKGDAGGEAG
                     GAAQPPAPKRSLRLFSQGLLTQLSNPKALVFFTALLPQFVSPEGRVFEQFLLLGVVSI
                     TVECPVLLAYGWLAERGQRLLPKGRLSTLPDRIAGAFLIGAGLGLAALRR"
BASE COUNT          635 a         1450 c         1585 g          747 t
ORIGIN      
        1 tggtgatcgt gctcttcccc ggcatcgggc tgaagatcaa cgggcagcgg gcgtggatcg
       61 gcgtccccgg cctcggccag ttccagccgt cggagttcat gaagattccg acggtgctca
      121 tgctcgcgcg ctacttcggc agaccgcgcc aatcgacgct gcgtctgaag gaagtggtgg
      181 cgggcggcct catcctcgcg ctccccgtgg cgctcatcct gcttgagccg gacgcggggc
      241 aggcgctcac ctacatcccg ctcctcgcgg tcgtcctctt tttgtcgtcc gtcaggatgt
      301 ggctcgtcgt cgcggccatc gcgctcgcca ccgtcgcggt gccggcggcc tacgtcgtcg
      361 gcgtccagac cggcgccatc aagggctacc agcaggagcg catcaacgtc atcctcgacc
      421 ccgagaacgc cgaccgccgc ggcttcggct accacacctg gcagtccatc gtcaccgtcg
      481 gcaagggcgg cctcaaaggc tccggcaccg cgggcgacgc ctcgcagagc cgcctcaaat
      541 tcctccccga gccgcacacc gacttcatct tcgccgtcac cgccgagaac acaggcttcg
      601 tcggctcgat cctcctcctg ctcgcctacg ccgtcctgct cacgaaactc atccacggcg
      661 cgcgccgcgc gcccgaccgc accggcatgc tcgtcatcat ggcgattgtc ggcggcctcg
      721 ccttccagat cctcatcaac gtcggcatgg ccctcggcat cctccccgtc atcggcgtcc
      781 cgctccccct catgagcgcc ggcctctcgg ccatcctcgc cacctcggtc gccatcggct
      841 tcgccatcag catccgcctg cgccgctacg tcaactagtt atgaccatct ttacggacgg
      901 acgatgaacc gacccggaac gagccgcagg ggtttcctcc gctccgctct ttcggcgggc
      961 gcattagtcg ccgggagcgg cggctggcct gcacgcgctt tcgcggcgga gagcagggcg
     1021 gagcggcggt tcgcgccggt gcgggtttcg cgggagaggt tgattcgcac cgtcgtcggg
     1081 ttgaggccgt accgggccga ggggttcgtc gtgaaggcgg agcgtgtggg cgagaagctg
     1141 gtcgtccaca attacgggca cgggggcgcg ggcgtgacgc tgtcgtgggg gacggcgaag
     1201 ctggcggcgg acgaggtgcg cggcgtcggc gggcaggaga ggttcgcggt tgtggggtgc
     1261 ggggtgagcg ggctgtcgtt ggcgcgcgtg ctccagcggc gcgggggcgc ggtgacgatc
     1321 tacgcgcggg agttgccgcc ggagacgacc tcgaacgtgg cgggggcgct gtggctgccg
     1381 acttcaagct acaacccgca gcgtgtgagc gcggcgtttc cggagcagtt ccgcgcggcg
     1441 tgcgaggtct cgaacagggc ttttcagttg atggtcggcg cggagtacgg cgtgcgctgg
     1501 atggagacgt acacggtgca cacgacggag gaggaggcgg cgcgggagct gctcggcggg
     1561 gcgcgtctgt acccggagac gaagcttcac cgcgacgcga agcgctactt cggcgcgccg
     1621 ctcgtgcgcc agttcagcac gatgctgatc gagccgtccg tctacctgaa cgcgctgctg
     1681 cgggactttc acgcggcggg cgggagggtg gtcgtgaagg agctgcgcgg gcgcgaggag
     1741 ttggcggcgc tcgccgagcc ggtcgtcttc aactgcgcgg gcctcggcgc gcgggcgctc
     1801 ttcgccgacg aggggctggt gcccgtgcgc gggcagctcg aagtcttgct gccgcagccg
     1861 gaggtcgatt actgtctgct caacggctcc tactacatgt tcccccggcg cgacggcatc
     1921 atcctcggcg ggacgttcga gcgcgacaac tggtcgctcg aaccgaacgc ggagacgacc
     1981 gcgctcatcc tcgacgcgaa cgccgaagcg atgaagcgcg tgcggcgttg attcgttgtt
     2041 caagcgagga cgtgagtgac gacgcgcgtc cgccgtcgag ttctgcaagc agctcgtccg
     2101 atgttcgtaa gaagcatgtc ggtcgtcgat tttcgcaaag ggagaaggcg atgaggttga
     2161 tgattctcgc ggtcgcgctc ttcgtcgcgg cggggctgac gacgttcccc gccgccgata
     2221 aggcttccgc tcgtcgtcac gcgcaggccg cggcgggcgc ggtcacgccg tgcgacgcgc
     2281 aggcggtcgt caacgacccc gacccgcagg gcatgaacgt gcgctcgggg ccgggcggca
     2341 ctttcaaggt catcgggaat ctgccgaacg cggacgtgaa cggcatcagc gtccacatca
     2401 ccggcgcgca gggcgagtgg gtgcgcatcg accgcgccga cgagcagggc ggcgagccgg
     2461 aggatcgggt cttcttcaag gggttgggtt gggtctacgc gccgctgctc acggtggacg
     2521 gcgtaggggg gatcgcgggc gggacgaagc tctacaagga gccttcggcg aagagccgcg
     2581 tcgtcgcgcg catgccggtg gacgtgggcg gcgccacggt gcacggctgc aagggcaagt
     2641 ggctctacgt cgagcacaag aaggtgcgcg ggtgggccgc gcccggcacc acctgctcga
     2701 acgcgctgac caattgcaac taacgggcat gaagggtctc aggaggcaca cgcacgaagg
     2761 gcggcggcgc gtcgtcgagg ggttggcgcc gctcgtccgc ggcgagtacc gtgacctgcc
     2821 gaagctgcgc gagacggcgg agagagtctt cgaggagttc gaagggattt tcgaggcgcc
     2881 gggcgtcgag ctttacgacg agagcgttga cccgaatgac gacccgccgc cgcgcgtccg
     2941 ttccggatga tgcagatgtc tcttgaggtc atactcgtct tcgccgccac ggagttcgtg
     3001 ctctccctga cgcccggccc cgccgtgttg gtcgtcgtct cgcaggggat gcggcacggg
     3061 ttcgcggcga gcgcgcgcgg cgcggcgggc atcctcgcgg gcaacgccgt ctacttcgcg
     3121 ttgtccgcgc tcggcctcgg cgcgctgctg ctggcgtcgg cgacgctctt cgccgtgctc
     3181 aagtgggcgg gcgtcgcgta cctcgtcttc gtcggcttga ggatgctgct cgcgaagggc
     3241 gacgccgggg gcgaggcggg cggcgccgcg cagccgcccg cgccgaagcg ttcgctgcgg
     3301 ctcttctcgc agggtctgct gacgcagctc tcgaacccga aggcgctcgt cttcttcacg
     3361 gcgctgctgc cgcagttcgt ctcgccagag ggccgcgtgt tcgagcagtt cctcctgctc
     3421 ggcgtcgtct cgatcacggt cgagtgcccc gtgctgctgg cctacggctg gctcgccgag
     3481 cgcgggcaga ggctcctccc gaagggccgg ctctcgacgc tgcccgaccg catcgcgggc
     3541 gcgttcctca tcggcgcggg gctggggctg gccgcgctgc ggcgctgaat cgaagaaggg
     3601 ggaaaagggg aaaaggggaa agggcgaaaa gggagacagg aaacagcgtg cctctcatcc
     3661 ttgaatggtg agaggcacgc tgttttcagc catcgactga ggataagaag cacgctgctt
     3721 ctcatcctcc cttttcgcct cttccccttt tccccctagt tgcggcggct cgccttttgc
     3781 gcacgcgctc gtttatactg gccgcgcctg tccttttggc ctgaacctaa aaccttccgg
     3841 cacggcgcgc gaagcagctt gagtcttagc cgaagtcgcc gcgcgtctat cgaaattgac
     3901 gggtcacagt tttcgaggag tcggcgaacc gtgcgcgctg cgtttatcga gacggcgcac
     3961 ggggccggcc ccgatttgaa agacggtagt ttgaagaggc cgccgggcgg cgtcggcgaa
     4021 gggcgggcac gcttgttaag cgcgccgtcc gccgcacgtc gccaacggga ggacgcgaag
     4081 gccgggccgg ggttgctcga aagctttgcc gcgcgacgca ccgagagttt catggtgcgc
     4141 gcgcggcaag cggaagaggc gccctcggcc cgcggcctga cgctccgaaa gcggcggccc
     4201 tcggacggag tttccaggtg aacagtggta tggctcccgg cgccggagga ggtcttcccc
     4261 tcacgccggg tgagcactgc ggcggcggcg acgcgcgcga ccccgaaggg gccgcgccgg
     4321 acgcgtcgtc gtagtccgtc tatacttcgg cgatctcact cctcgaaagc tcctgccggc
     4381 actttccgtc ccagcaccgg gccgtgcgac cgcggcg
//
LOCUS       DBNGOH010000298         2770 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2977939, whole genome shotgun sequence.
ACCESSION   DBNGOH010000298 DBNGOH010000000
VERSION     DBNGOH010000298.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2770)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2770)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2770
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2977939"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            236..448
                     /gene="rpsU"
                     /locus_tag="AC159P_05765"
     CDS             236..448
                     /gene="rpsU"
                     /locus_tag="AC159P_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HIR3694995.1"
                     /translation="MATVNVNQGESIESALRRFKRKVMSEEIIKDAKKHAFFMSRGQK
                     AKLKSALARKRNKRKQRPQRPRPPRG"
     gene            complement(692..2632)
                     /locus_tag="AC159P_05770"
     CDS             complement(692..2632)
                     /locus_tag="AC159P_05770"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015143569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HIR3694996.1"
                     /translation="MSQTKEEPYGSWPSPITSDMIVGATVGLGQVALDGEDTYWIELR
                     PAEGGRNCLVRRTPDGQTADVTPPDFNARTRVHEYGGGDFAVRGGTVFFSNFKDQRLY
                     RQDAGGAPRPITPEKDYRYSDPVFDARRNRLLAVREDHTVEGREAVNMIVALDAGGAN
                     EDGGRVLVSGNDFYASPRLSPDGSRLAWQTWNHPNMPWDGCELWVAEVGENGSPAGAE
                     LVAGGEEESIFQPEWSPDGTLYFASDRSNWWNLYRRGADGAVEPVCEREAEFGTAQWV
                     FAMSAYSFASAERIVCAYNEKGTWSLAALDTKTGRLARIETPYTEISYVKAAGDRVVF
                     RGGSPTNRLAVVEVDLRSGAHNVLRRASEVVVDEGYLSIPRAVEFPTENGLTAHGFYY
                     PPRNRDYAAPAGERPPLVVRCHGGPTATTTTTLRLDMQYWTSRGVAVLDVNYGGSTGY
                     GREYRKRLNGQWGVVDVDDCVNGARFLIDQGEVDGGRCVITGGSAGGYTTLNALTFRD
                     LFKAGASHFGISDLTVFVGDTHKFESRYLDRLVGPYPELADLYFERSAINFTDRLSCP
                     IIFFQGLEDKVVPPNQAELMVEALRRKKLPVAYLPFEGEQHGFRKAENIKRALEGELY
                     FYSRVFGFPLADDVEPVEIENL"
BASE COUNT          447 a          890 c          952 g          481 t
ORIGIN      
        1 ccctttcccc ttttcccctt ttccccctgg ctatttcctg tgcgtttgcc agaagcggtt
       61 gcgggctggc acaatagcgg ttcgctccga ggagcggatg agagagaccg agaggcaggt
      121 gttgcccgaa ctatcgttaa agtaatttaa gtcgcatcag gttaatcgta gacgtttcgc
      181 gtgggcgccg tgcgccgcgc gggtttcgag ttacttttta ttggagaagt ccgaaatggc
      241 aacagtgaat gtgaatcagg gcgagtcgat cgagagcgcc ctccgccgct tcaagcgcaa
      301 ggtgatgtcc gaggagatca tcaaggacgc gaagaagcac gccttcttta tgtcgcgcgg
      361 ccagaaggcc aagctcaagt cggcgctcgc gcgcaagcgc aacaagcgca agcagcgccc
      421 gcagcgcccg cgtccgcccc gcggctagag acgcttcggc cagcgacgaa aagagaaagc
      481 gcacgggttg attcccgtgc gctttctctt ttctcgaatt tagagcgggt ttcggttgac
      541 cgtagaacgc gcgcgaaagc tgtaggggca gggcttgtcc ctgcccgcac gcacgcgatg
      601 atgcgacgtt gaattcatcg agtcaggcgg gcagggacac gccctgcccc tacagccatg
      661 acgaacgtcg cgcaatcttt cgataacggc tctaaaggtt ttcgatctcg accggctcca
      721 cgtcgtccgc gagcgggaag ccgaagacgc gcgagtagaa gtagagttcg ccttcgagcg
      781 cgcgcttgat gttctcggcc ttgcggaagc cgtgctgctc gccctcgaag gggaggtagg
      841 cgacgggcag cttcttgcgg cgcagcgcct cgaccatcag ctcggcctgg ttcggcggca
      901 ccaccttgtc ttcgaggccc tggaagaaga tgatcgggca cgagagccgg tcggtgaagt
      961 tgatcgcgga gcgctcgaag tagaggtcgg cgagctcggg gtaggggccg acgaggcggt
     1021 cgaggtagcg cgactcgaac ttgtgcgtgt cgccgacgaa gaccgtcagg tcgctgatgc
     1081 cgaagtggct cgcgcccgcc ttgaagaggt cgcggaaggt gagcgcgttc aaggtcgtgt
     1141 agccgcccgc gctcccgccc gtgatgacgc agcgcccgcc gtccacctcg ccctgatcga
     1201 tgaggaagcg cgcgccgttc acgcagtcgt ccacgtccac cacgccccac tgcccgttca
     1261 gacgcttgcg gtactcgcgc ccgtagccgg tgctgccgcc gtagttcacg tcgagcacgg
     1321 cgacgccgcg gctcgtccag tactgcatgt cgaggcggag cgtggtggtg gtggtcgcgg
     1381 tcgggccgcc gtggcagcgg acaacgagcg gcgggcgctc gcccgcgggc gccgcgtagt
     1441 cgcggttgcg cggcgggtag tagaagccgt gcgcggtcag tccgttctcg gtcgggaact
     1501 cgaccgcgcg cgggatcgaa agataacctt cgtccacgac gacttcgctc gcgcggcgca
     1561 gcacgttgtg cgcgccgctc ctgagatcga cctcgacgac ggccaggcgg ttggtcggcg
     1621 acccgccgcg gaagacgacg cggtcgcccg ccgctttgac gtatgagatt tcggtgtagg
     1681 gcgtctcgat gcgcgcgagc cgcccggtct tcgtgtcgag cgcggcgagg ctccacgtgc
     1741 ccttctcgtt gtaggcgcag actatccgct cggcggacgc gaacgagtaa gccgacatgg
     1801 cgaagaccca ctgcgccgtg ccgaactcgg cctccctctc gcacacaggc tcgaccgcgc
     1861 cgtccgcccc gcgcctgtag aggttccacc agttgctgcg gtcggaggcg aagtagagcg
     1921 tgccgtcggg cgaccactcg ggctggaaga tcgactcctc ctcgccgccc gcgaccagct
     1981 cggccccggc gggcgacccg ttttcgccga cttcggcgac ccacaactcg cagccgtccc
     2041 agggcatgtt cgggtggttc cacgtctgcc acgcgagccg cgagccgtcg ggactcaagc
     2101 gcggcgaggc gtaaaagtcg ttgcccgaaa cgagcacgcg cccgccgtct tcgttcgcgc
     2161 cgccggcgtc gagcgcgaca atcatgttca cggcttcacg cccctcgacg gtgtggtctt
     2221 cgcggacggc gagcaggcga ttgcgtcgcg cgtcgaagac ggggtcgcta tagcggtagt
     2281 ccttctccgg cgtgatcggc cgcggggcgc cgcccgcgtc ctgacggtag aggcgctggt
     2341 ctttgaagtt ggagaagaag accgtgccgc cgcggacggc gaagtcgccg ccgccgtatt
     2401 cgtggacgcg cgtgcgcgcg ttgaagtcgg ggggcgtcac gtcggcggtc tgcccgtccg
     2461 gggtgcggcg gacgaggcag ttgcgcccgc cctccgcggg ccgcagctcg atccagtagg
     2521 tgtcttcgcc gtcgagcgcg acctgcccga ggccgaccgt cgcgccgacg atcatgtccg
     2581 aggtgatcgg cgacggccag gagccgtaag gttcttcctt cgtttgactc attaaaaacc
     2641 ctccgcatgt tggtttcgaa cagagcttca gcgaccgaat ctgtcgggcg gcggcgcgac
     2701 cgcgcagaag tccgtgccga agcgcgcggc gtactgcgcg agcagctctc gcacgcgcgg
     2761 gagctgcggc
//
LOCUS       DBNGOH010000299         3674 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_44937, whole genome shotgun sequence.
ACCESSION   DBNGOH010000299 DBNGOH010000000
VERSION     DBNGOH010000299.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3674)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3674)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3674
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_44937"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..366)
                     /locus_tag="AC159P_05775"
     CDS             complement(<1..366)
                     /locus_tag="AC159P_05775"
                     /inference="COORDINATES: protein motif:HMM:NF024762.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HIR3694997.1"
                     /translation="MTPADKLGAAEARGRFAAEVSKGAAIDLGRAALLVGAEEEPAGC
                     DVGRCVESLERMGREAAERIRDGGASAVEALNRYLFEERGFAGNQTDYYDPRNSMLHR
                     VLERRTGIPITLSVVYMEVG"
     gene            complement(442..1149)
                     /locus_tag="AC159P_05780"
     CDS             complement(442..1149)
                     /locus_tag="AC159P_05780"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR3694998.1"
                     /translation="MIQAVMFDFNGVIINDEPLHQKAFREALAAEGVGLSEAEYMTML
                     GMDDATFVRTAFERAGVELTEERMRAVIEREGATHRAMLEETAELPLFPGVVTFVKAL
                     ASKHTLGLVSMAERAEIEYGIGRALIAPYFSSIVSAEDVQACKPDPQCYLLALERLNK
                     KRGEAAAQVIPLRPSECLVVEDSPPGILSARAAGMRTLGVTNTVKDKALREAGAEVVT
                     GNLSDWTVDAVHHLYDK"
     gene            complement(1348..2226)
                     /locus_tag="AC159P_05785"
     CDS             complement(1348..2226)
                     /locus_tag="AC159P_05785"
                     /inference="COORDINATES: protein motif:HMM:NF017970.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HIR3694999.1"
                     /translation="MSDNAPQFMTLEALRSVPLFASLDDRAAHALRAHVEARRVPAGT
                     RLFRLGDTGDRMYLIEGGRVRIHVHDSDGDDMTLAELGDGDFFGEMAILDGKPRSAHA
                     TVVQDADLGTISRDDFLDFVRTNPDVALKMMSAITERLRRTDELLRTRVSRNLNEVEE
                     ERQTLADRMADALSDFGGSWKFLCATILFVAFWMFYNSWWRQNAGLDPFPYSFLDVIN
                     GVVGALLAPIILISQNMQENKDRLRADLDYQVNLKNELAIAEVMRRLDVLESERLPLL
                     FEEQNRQIREAVGGRQ"
     gene            complement(2455..2793)
                     /gene="trxA"
                     /locus_tag="AC159P_05790"
     CDS             complement(2455..2793)
                     /gene="trxA"
                     /locus_tag="AC159P_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014098536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HIR3695000.1"
                     /translation="MKSVAEVSDGTFESDVLGAERVTVVDFGATWCPPCRALEPTIEE
                     LAKKYEGRVDFLKLDIDANPSTPQRYGIKGIPTLIVFDSGRELERMVGAASKDAISRV
                     VDKYIPAAVA"
     gene            2984..3649
                     /locus_tag="AC159P_05795"
     CDS             2984..3649
                     /locus_tag="AC159P_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HIR3695001.1"
                     /translation="MTNENSQRRPPAPDLFRDFNAEEMREVERVTATRRFQRGAVLYT
                     PGETGEALFLLREGAVQIYRMSPEGRKLVIAHLLPFSFFGEMTCIGQGMYDTYAEVTE
                     DSVLLMMNCADLERLLVSKPQVARRILEAFGRRVLEAERQLEDIAFKGMAARVAAQLL
                     READGTEVRGLSHQDIAERLGVYRETATNALNELKNASIIRIGRRHISILDRRRLERV
                     AAS"
BASE COUNT          622 a         1199 c         1194 g          659 t
ORIGIN      
        1 cccgacctcc atgtacacga ccgagagcgt gatcgggatg cccgtccgcc gttcgagcac
       61 gcggtggagc atgctgttgc gcgggtcgta gtagtcggtc tggttgccgg cgaagccccg
      121 ctcttcgaac aggtatcggt tgagcgcctc gaccgccgat gcgcccccgt cgcggatgcg
      181 ctcggcggcc tcgcgcccca tcctttccag agactccacg caccgcccca cgtcgcaccc
      241 cgccggctcc tcctccgcgc cgacgagcaa cgccgcgcgc ccgaggtcga tcgccgcgcc
      301 cttcgacacc tccgccgcga agcgcccacg cgcctccgcc gcgcccaatt tgtcagcggg
      361 tgtcataaaa gaagtgatga atgcggaatg atgaatgatg aataaaaagc gatttgtctt
      421 tcattccgca ttccgcattc atcatttgtc gtagaggtgg tggacggcgt ccaccgtcca
      481 gtccgagagg ttgccggtga cgacttcggc gccggcctcg cggagcgcct tgtctttgac
      541 cgtgttggtg acgccgaggg tgcgcatgcc cgcggcgcgc gcggagagga tgccgggcgg
      601 cgagtcttcg acgacgaggc actccgaggg gcggagcggg atgacctgcg ccgccgcctc
      661 gccgcgcttt ttattaaggc gttcgagcgc gagcaggtaa cactgcgggt cgggcttgca
      721 cgcctgcacg tcctcggcgc tgacgatgga agagaagtag ggggcgatca gcgcgcggcc
      781 tatgccgtac tcgatctcgg cgcgctcggc catgctgacg aggccgagcg tgtgcttgct
      841 cgcgagcgcc ttgacgaacg tcacgacgcc ggggaagagc ggcagctccg ccgtctcttc
      901 gagcatcgcg cggtgcgtcg ccccctcgcg ctcgatgacg gcgcgcatcc tctcctccgt
      961 cagctcgacg cccgcgcgct cgaacgcggt gcgcacgaac gtcgcgtcgt ccatgccgag
     1021 catcgtcatg tactccgctt ccgacagccc cacgccctcc gccgccagcg cctcgcggaa
     1081 agccttctgg tgcagcggct cgtcgttgat gatgacgccg ttgaaatcga acatcaccgc
     1141 ttggatcatt gatgtactaa ggatgacagc tctcgtggag tggataaagc acgcacagta
     1201 tacagtaggc ggtaggcagt aagaagacgg gagtcaggag tcagaagaag gaagaggctt
     1261 gggaggtaaa gtccaagcct ctccttgctt ttgtctttac tgcctgctgc ccgctgcctg
     1321 ctgcctgctt ccttttctgc ctaccgccta ctgcctaccg cctactgctt cccgtatctg
     1381 cctgttctgc tcctcgaaga ggagtgggag ccgctcgctt tcgagcacgt cgaggcggcg
     1441 catgacttcg gcgatggcga gttcgttctt gaggttgacc tgatagtcga ggtctgcgcg
     1501 gaggcggtct ttgttctcct gcatgttctg cgagatgagg atgatgggcg cgagcagcgc
     1561 gccgacgacg ccgttaatga catcgaggaa cgagtagggg aaagggtcga ggcccgcgtt
     1621 ctgccgccac caggagttgt agaacatcca gaaggcgacg aagaggatgg tcgcgcagag
     1681 gaacttccaa ctgccgccga agtccgagag ggcgtcggcc atgcggtcgg ctaacgtctg
     1741 gcgctcctcc tcgacctcgt tgaggttgcg cgagacgcgc gtgcgcagca gctcgtccgt
     1801 gcgccgcagc cgctcggtga tggcgctcat catcttcagc gccacatccg ggttggtgcg
     1861 gacgaagtcg aggaagtcgt cgcgcgagat ggtgccgagg tccgcgtcct gcacgacggt
     1921 cgcgtgcgcc gagcgcggct tgccgtcgag gatcgccatc tcgccgaaga agtcgccgtc
     1981 gcccagttcg gcgagcgtca tgtcgtcgcc gtcggagtcg tggacatgga tgcggacgcg
     2041 ccctccctcg atgaggtaca tccggtcgcc cgtgtccccc aggcggaaga gtcgcgtgcc
     2101 cgccggcacg cgccgcgcct cgacgtgggc gcgcagggcg tgcgccgcgc ggtcgtcgag
     2161 cgaggcgaag agcggcaccg agcgcagcgc ttcgagcgtc atgaactggg gggcgttgtc
     2221 cgacatgaat ttcacctcac gggaatttga ttcacggcga cgagcgatca cgccgccgtc
     2281 caccgttccg aagaactcgg cgaaatcata aaacatcacg cgacgcggga agaaaatttt
     2341 tccggcgatg gtgcggacga acaaaaaggg gcgctgtcga agttcatgaa cttcgacagc
     2401 gcccctttac ttaaacgcgg gcagaggtct gcacttgtgg ccgcgcggcg ctgctcaggc
     2461 gaccgcggcg gggatgtact tatcgacgac gcgcgagatg gcgtctttgc tggccgcgcc
     2521 gaccatgcgt tcgagttccc tgccgctgtc gaagacgatc agcgtcggga tgcccttgat
     2581 gccgtagcgc tgcggcgtcg aggggttcgc gtcgatgtcg agcttcagga agtccacgcg
     2641 cccttcgtac ttcttcgcca gctcttcgat ggtcggctcc agcgcccgac acggcgggca
     2701 ccacgtcgcg ccgaaatcga cgaccgtcac cctctccgcc cccagcacgt cgctctcgaa
     2761 cgtgccgtcg ctcacttccg cgacactttt catgttctcg aaatctcctt cgtctaccgg
     2821 cggcgctcgt gcttgcaggc gccgcccgct caagcttcga tcccaggtat catgcgcctt
     2881 gccggcggcc tccgtcagta atgctcgtta cagttcgcgc cccccgcctg tggatactgt
     2941 ggcgcggggc cgctttgtgt aaaatggctg ccggattgaa gagatgacga acgagaacag
     3001 ccagcggcgc ccgcccgcgc ccgacctctt ccgcgacttc aacgccgagg agatgcgcga
     3061 ggtcgagcgc gtcaccgcga cgcgccgctt tcagcgcggg gccgtgctct acacgccggg
     3121 cgagacgggc gaggcgctct tccttctgcg tgagggcgcg gtgcagatct atcgcatgtc
     3181 gcccgagggg cgcaaactcg tcatcgcgca cctcttgccc ttcagcttct tcggcgagat
     3241 gacgtgcatc gggcagggca tgtacgacac ttacgccgag gtgacggagg actcggtgct
     3301 gctgatgatg aactgcgccg acctcgaacg cctgctcgtc agcaagccgc aggtcgcgcg
     3361 ccgcatcctc gaagccttcg gcaggcgcgt gctcgaagcc gagcggcagc tcgaagacat
     3421 cgccttcaag gggatggcgg cccgcgtcgc agcgcagctg ctgcgcgagg ccgacggcac
     3481 ggaggtgcgc ggcctctcgc atcaggacat cgccgaacgc ctcggcgtct accgcgagac
     3541 ggcgaccaac gcgctcaacg agctgaagaa cgcctcgatc atccgcatcg gcagaaggca
     3601 catctccatc ctcgaccgcc gacgcctcga acgcgtcgct gcctcataaa gaggaggggg
     3661 aaaaggggaa aagg
//
LOCUS       DBNGOH010000300         2246 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_595311, whole genome shotgun sequence.
ACCESSION   DBNGOH010000300 DBNGOH010000000
VERSION     DBNGOH010000300.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2246)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2246)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2246
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_595311"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            364..2034
                     /locus_tag="AC159P_05800"
     CDS             364..2034
                     /locus_tag="AC159P_05800"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013126.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 protein"
                     /protein_id="HIR3695002.1"
                     /translation="MTGRDDKAEARWVEETLKSLSLRERVGQLVMTPVQVGPATVAGE
                     RFDELRRQVEDNGLGGVIVRAGSPAEVAALTNKLQERAKVPLFVAADYERGLRMQMKT
                     GTPFTNNMGVAAAGDVQAAYRQGKITAGEMRAIGVNWSFGPVADINNNPDNPVINVRS
                     FGEDPRRVAAFVAAAVKGLRDGGVLSTAKHFPGHGDTATDTHIGLATIKADRARLDRV
                     ELVPFRAAIAADVDAVMTAHVAAPAITGDELPATLSAKMTTDLLRRELKFDGLVITDS
                     LGMGAITRGYPGGEAAARAIKAGADIALSPPDTKAAIEAIEEFVRRGELTEARIDASV
                     RRILRAKHRLGLAARRTVDASAVGQHVERPDATEEARRTAENSITLLRNENGLLPLDP
                     SRAARTLFVVVAADEDPEEGRTFVPEVTRRAPAARILRAEPRTSSADYDAMLEDCAKA
                     DAVVVAAFVKRAAGKGTVALPEAQAEFVRRLVATKRPVAVVAFSGPYLIKHFPDATVY
                     AAAYAIEDVAQAAAVRVLFGEAPFRGRLPVSVPGLFDIGAGIQSRAKR"
BASE COUNT          378 a          818 c          746 g          304 t
ORIGIN      
        1 gacgggctgc tgcgtgatct cttcgagcat cagggacacg gcttcttcgt ctccaggggt
       61 atacaggttg ggcgagcgag cgcgaactcg ttgacagcgg tttgccaaag tgctaattgt
      121 cggcgcaagc gttcgcacgc attatccacg aagacggcaa acattatcaa aaagcaaacg
      181 caccccgccg cacgacacac gcgcctcggc ctccgattac cgcgccgggg cgtagaccca
      241 acacgcgcac gcacgcgaca caggccgcgc actttaccct tcgtcttcgc attcatcgtt
      301 tgcgccgccg cactctgcgc ggcgttcgcg gcgcagacga cgacgcgcga gcggcgtcga
      361 tacatgacgg gccgcgacga caaagcggag gcgcgctggg tggaagagac tttgaagagc
      421 cttagcctgc gcgagcgcgt cgggcagttg gtgatgacgc cggtgcaggt cggccccgcg
      481 acggtcgcgg gcgagcggtt cgacgagctg cgcaggcagg tcgaagataa cggcctcggc
      541 ggcgtcatcg tgcgcgcggg cagccccgcg gaggtcgccg cgctgacgaa caagctacag
      601 gagcgcgcga aggtgccgct cttcgtcgcc gcagactacg agcgcggcct gcggatgcag
      661 atgaagacgg gcacgccctt cacgaacaac atgggcgtcg cggcggcggg cgacgtgcag
      721 gccgcctacc gtcagggaaa gatcaccgcc ggggagatgc gcgccatcgg cgtcaactgg
      781 tctttcggcc ccgtcgcgga catcaacaac aaccccgaca accccgtcat caacgtccgc
      841 agtttcggcg aagacccgcg ccgcgtcgcc gcgttcgtcg ccgccgcggt caagggcctg
      901 cgcgacggcg gcgtgctctc gaccgcgaaa cacttccccg gccacggcga cacggccacc
      961 gacacgcaca tcggcctcgc caccatcaag gccgaccgcg cgcgcctcga ccgcgtcgaa
     1021 ctcgtccctt tccgcgcggc catcgcggcg gacgttgacg cagtgatgac cgcgcacgtc
     1081 gccgcgcccg ccatcaccgg cgacgagctg cccgcgacgc tctccgcgaa gatgacgacc
     1141 gacctgctgc gccgcgagct gaagttcgac ggtctcgtca tcacggactc gctcggcatg
     1201 ggcgccatca cgcgcggcta tccgggcggc gaggccgccg cgcgcgccat caaggcgggc
     1261 gccgacatcg cgctctcgcc gccggacacg aaggccgcca tcgaagccat cgaagagttc
     1321 gtcaggcgcg gcgagctgac cgaggcgcgc atcgacgcga gcgtccgccg catcctccgc
     1381 gccaagcaca ggctcggact cgccgcgcgg cgcacggttg acgcctccgc cgtcggccag
     1441 cacgtcgagc gccccgacgc gacggaggag gcgcggcgga cggcggagaa ttcgatcacg
     1501 ctcctgcgga acgagaacgg acttttgccg ctcgatccgt cgcgcgccgc ccgcacactc
     1561 ttcgtcgtcg tcgccgcgga cgaagacccg gaggagggcc gcaccttcgt ccccgaagtc
     1621 acgcgccgcg cacccgccgc gcgcatcctg cgcgccgagc cgcgcacctc ttccgccgac
     1681 tacgacgcga tgctcgaaga ttgcgcgaag gcggacgcgg tggtcgtcgc cgcgttcgtg
     1741 aagcgcgcgg cgggcaaggg caccgtcgcg ctgcccgagg cgcaggccga gttcgtgcgc
     1801 cggctcgtag cgaccaagag gccggtcgcg gtcgtcgcct tcagcggccc gtacctcatc
     1861 aagcatttcc cggacgcgac ggtctacgcc gccgcctacg ccatcgagga cgtggcgcag
     1921 gctgccgccg tccgcgtcct cttcggcgaa gcccctttcc gcgggcgcct gccggtgagc
     1981 gtgccggggc tgttcgacat cggcgcgggc attcagtcgc gcgcgaagag atgaatcgtt
     2041 cctcgcccgt cattcaacga taaagcggaa gcgccaatga cgacatccac atcatccacg
     2101 cacacgggtg ggacgcaggc cgaccgcctc gtctcgctgg acgcgttccg cgggctgacc
     2161 atcgcgggca tgatcctcgt caacaacccc ggcacgtggg gcgccatcta cgacccgctg
     2221 gagcacgccg cgtggcacgg ctggac
//
LOCUS       DBNGOH010000301         1752 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1879038, whole genome shotgun sequence.
ACCESSION   DBNGOH010000301 DBNGOH010000000
VERSION     DBNGOH010000301.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1752)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1752)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1752
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1879038"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..558
                     /gene="atpB"
                     /locus_tag="AC159P_05805"
     CDS             <1..558
                     /gene="atpB"
                     /locus_tag="AC159P_05805"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HIR3695003.1"
                     /translation="DNVGPRGMKYFPIIATFAVLILISNLMGLVPGLMSPTASTSVTF
                     ALGITSFVYYNYIGISENGLVGHLKHFAGPIPWLAFLMFPIELISNLVRPMSLGIRLF
                     GNLFGDEQILGTIAGLVPWVAPVIIMPLSAFVAFMQTFIFVLLSIIYISEVTHHHEEG
                     HGEHGDPHHDTPALTDGEQGVVAAP"
     gene            886..1200
                     /locus_tag="AC159P_05810"
     CDS             886..1200
                     /locus_tag="AC159P_05810"
                     /inference="COORDINATES: protein motif:HMM:NF012365.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HIR3695004.1"
                     /translation="MRKLRYAFFAVLTVLMSAAPVLAQAAAPDNASSEAKFGKIAAGF
                     GFAIAAALAAIGQSRGAAAAAEGVARPPGAGARIQTMMILGLVFIETLVLFTLVIVFV
                     KI"
     gene            1357..>1752
                     /locus_tag="AC159P_05815"
     CDS             1357..>1752
                     /locus_tag="AC159P_05815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695005.1"
                     /translation="MKNAARILVVYAAAMLFAATAFAQDPAASPAAPAAPAPGQECET
                     AKTDPNLYPKWLEDRKGDAEKQKTAYAAGKEFLAKCSSAAADDAYVNAVKKWVASYEA
                     AVGKFEVRKKFEDAAKAVDAAQGPKDYTQL"
BASE COUNT          317 a          562 c          584 g          289 t
ORIGIN      
        1 gacaacgtcg ggccgcgcgg gatgaagtac ttcccgatca tcgccacgtt cgccgtgctg
       61 atcctgatct cgaacctgat ggggctggtg ccgggcttga tgtcgcccac ggcctcgacc
      121 tccgtcacgt tcgcgctcgg catcacctcg ttcgtctact acaactatat cggcatcagc
      181 gagaacggac tggtcggcca cctcaagcac ttcgcggggc cgatcccgtg gctcgcgttc
      241 ctgatgttcc cgatcgagct gatcagcaac ctcgtgcgcc ccatgtcgct gggcatccgt
      301 ctcttcggga acctcttcgg cgacgagcag attctcggca ccatcgcggg cctcgtgccg
      361 tgggtcgcgc ccgtcatcat catgccgctg agcgccttcg tcgccttcat gcagacgttc
      421 atcttcgtgc tgctctcgat catctacatc agcgaggtga cgcaccacca cgaagaaggc
      481 cacggcgaac acggcgaccc gcaccacgac acgcccgcgc tgacggacgg cgagcagggc
      541 gtcgtcgccg cgccgtagcg gattcgattc gggcgcgccc gtcgagcggc gcgggcacac
      601 gcgcccgcgc gcgttggggc gctaaggcag agaacaggac tcaggctccg ggccgttcta
      661 cgggaggcga gggggcgggg caggaaatca cggaaacgcc gcgtagggct ttcgatgttt
      721 gagagcgacg ggtgccgaca tcgctgtgag cgccgttgta agaggggcgg cggataccgg
      781 gtggaggcga aatcataagt agcgcgcgcg tcgagttctc cccgttcttc agggcgtctt
      841 cgaggaagct tccggagctt ggaaactccc agaggagaag cagccatgag aaaactcaga
      901 tacgctttct tcgcggtctt gaccgtgctg atgtcggcgg cgcccgtgct ggcgcaggcg
      961 gcggcgccgg acaacgcctc ttccgaggcc aagttcggga agatcgccgc cggcttcggc
     1021 ttcgccatcg cggcggcgct cgcggccatc ggccagtcgc gcggcgcggc ggcggcagcc
     1081 gaaggcgtcg cgcgccctcc gggtgcgggc gcccgcatcc agacgatgat gatcctcggt
     1141 ctggtcttca tcgagacgct cgtgctcttc acgctcgtca tcgtgttcgt gaagatttag
     1201 cgtcggaagt aggaaaccgg tgcgggcgcg gtctacatca aacgtcgcgc ccgcaccgga
     1261 agccactgtg tcgggaatga agcgcctgcg cgagcggaca gccgggcgta aataaagtat
     1321 gagagacttg ctttagcccg gagggaattt caaccaatga aaaacgcagc gagaatcctc
     1381 gtcgtttacg ccgccgcgat gctcttcgcc gcgacggcct tcgcgcaaga cccggcggcg
     1441 tcgcccgccg cgcccgccgc gcccgcgccc ggtcaggaat gcgagacggc caagaccgac
     1501 ccgaaccttt acccgaagtg gctcgaagac cgcaagggcg atgccgagaa gcagaagacc
     1561 gcctacgcgg cgggcaaaga gtttctggcc aaatgctcca gcgctgccgc cgacgacgcg
     1621 tacgtgaacg ccgtcaagaa gtgggtcgcc tcgtacgagg cggcggtcgg caagttcgaa
     1681 gtacggaaga agttcgagga tgcggcgaag gcggtggatg ccgcgcaggg gccgaaggac
     1741 tacacgcagc tc
//
LOCUS       DBNGOH010000302         2963 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4994262, whole genome shotgun sequence.
ACCESSION   DBNGOH010000302 DBNGOH010000000
VERSION     DBNGOH010000302.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2963)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2963)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2963
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4994262"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1042)
                     /gene="bshC"
                     /locus_tag="AC159P_05820"
     CDS             complement(<1..1042)
                     /gene="bshC"
                     /locus_tag="AC159P_05820"
                     /inference="COORDINATES: protein motif:HMM:NF021586.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis protein BshC"
                     /protein_id="HIR3695006.1"
                     /translation="MSTTETACHSTPEGAGLRVELLPFERVPHQSRLFLDYLRDPTAL
                     RRFYPEAVRHHGELPARAARVLEAHRASRDALCDALADLYSSWGAGKETLENVARLRA
                     ADAVAVVTGQQVGLFTGPLYTLYKALSAVKLAACLSQRGTEAVPVFWMATEDHDWEEV
                     QTAEVIACDGRLAAVGVSSELHTEGEQVGGVTLDAGVEEASQRLFELMPSSEFLPDLE
                     ALVRDAYAPGRGFGEAFARMLAALTGRYGLVLLDPTDAALKRLAAPLYSEAARLGAEI
                     AAATDARSRELVESGYHAQVHTSPDSFPLFLIDGGGRRALARTKDGRYRAKGTGHEWT
                     AGELAALALAEPE"
     gene            complement(1210..>2963)
                     /locus_tag="AC159P_05825"
     CDS             complement(1210..>2963)
                     /locus_tag="AC159P_05825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695007.1"
                     /translation="SDDEITREDYIGIVQRIEREFLKGVQRNIRTRVLAEWVTNLKMI
                     FSASYSRTLRVGELYESELFARVPDGVKVEGPRWLPDWAAKLFGLRRDRRWLNDLYIH
                     PLLPDGTQQTDFKPRSHNWRRASKSPDLILNAATLNTGHNWQFTASWMGEPPTPINAD
                     IDCNYRLRRMYYENAPEPYRQFRLGHAVAASSCVPGLFEPLILDGLYPYDADGKLNDK
                     QRISVRLVDGGAVDNQGINSLLEQDCTVMLISDGSGQMDAQNVPDGGLLGPPLRTNSI
                     LQARIREAQYTDIASRRRSSLLRALMFIHLRQELTGENLAWKDCPSKYRESDFERADA
                     GRNDSTSFGVAPAVQRQLSAIRTDLDSFSDAEAYALMASAYRMTAIQFTGDKRTVEGF
                     AEPDTTPCWDFLKVEDAMRAESADGDMARRRHLERLLDVGSNIAFKIWMLSGALKAIG
                     YALAVAAVVGLVVLFYRMWEQPFQPMVYAGAAYEWVRQYLPFLPALPALTFGFIGGVV
                     ISTLVVTAITIVVNYYLGKRRGKLVMKAFNWRRTLTDIGVGLVMATVGFAAARLHLHV
                     FDKLFLRYGRLAKFPKS"
BASE COUNT          454 a          961 c         1080 g          468 t
ORIGIN      
        1 gctcgggctc cgcgagcgcg agcgcggcca gctcgccggc cgtccactcg tggccggtcc
       61 ccttcgcacg gtagcggccg tccttcgtgc gcgcgagcgc gcgccgcccg ccgccgtcga
      121 tgaggaagag cgggaaggag tcgggcgagg tgtgcacctg cgcgtggtag ccggactcga
      181 ccagctcgcg cgagcgcgcg tcggtcgcgg cggcgatctc cgcgccgagc cgggcggcct
      241 ccgagtagag cggcgcggcc aggcgtttca gcgccgcgtc ggtggggtcg agtaagacca
      301 gtccgtacct gcccgtcagc gccgcgagca tccgcgcgaa cgcctcgccg aagccgcgcc
      361 ccggcgcata cgcgtcgcgc acgagagctt cgaggtcggg caggaactcg ctcgaaggca
      421 tcagctcgaa gagccgctgc gaagcctcct cgacgcccgc gtcgagcgtg acgccgccca
      481 cctgctcgcc ctccgtgtgc agctcacttg agacgccgac cgcggcgagc cgcccgtcgc
      541 acgcgatgac ctcggccgtc tgcacctcct cccagtcgtg atcctcggtc gccatccaga
      601 agacgggcac ggcctccgtc ccgcgctgcg acaggcacgc cgcgagcttc accgccgaca
      661 gggccttgta gagcgtgtag agcggcccgg tgaagagtcc gacctgctgg cccgtgacga
      721 cggcgaccgc gtccgcggcg cgcaggcgcg cgacgttctc cagcgtctct ttcccagcgc
      781 cccacgacga atacagatcg gcgagcgcgt cgcagagcgc gtcgcgcgag gcgcggtgcg
      841 cttcgagcac gcgcgcggcc cgtgcgggca gctcgccgtg gtggcggacg gcctcggggt
      901 agaagcggcg gagcgcggtc gggtcgcgga ggtaatcgag gaagaggcgc gactggtgcg
      961 ggacgcgctc gaacggcagc agctcgacgc ggaggcccgc gccttccggc gtgctgtgac
     1021 aggctgtttc ggtggtgctc aaagcaggaa agagtatagc aaaagcggga gtcggaagtc
     1081 aggagtcggg gaatgaagag tgacggagct tgagaggtaa agtgctcgga ctccctctcc
     1141 ggccacacga tgaaagaaga ggcttgggac tttacctctc aagcctcttc ttcattccga
     1201 ctcctgactt cagctcttcg ggaatttggc gagccttccg tagcgaagga aaagtttgtc
     1261 gaagacgtgc aggtggaggc gcgcggcggc gaagccgacc gtcgccatga cgaggccgac
     1321 gccgatgtcc gtgagcgtgc gccgccagtt gaacgctttc atgacgagct tgccgcgacg
     1381 cttgccgagg tagtagttga ccacgatggt gatggccgtc acgacgagcg tcgagatgac
     1441 gaccccgccg atgaagccga aggtcagcgc gggcagcgcg ggcaggaacg gcaggtattg
     1501 ccgcacccat tcgtaggccg ccccggcgta gaccatcggc tggaagggtt gctcccacat
     1561 cctgtagaag agcacgacga ggccgacgac ggcggcgacc gccagcgcgt agccgatggc
     1621 cttgagcgcg cccgagagca tccagatttt gaaggcgatg ttcgagccga catcgagcag
     1681 ccgttcgagg tgacggcggc gcgccatgtc gccgtcggcg gactcggcgc gcatggcgtc
     1741 ttcgaccttg aggaagtccc agcagggcgt cgtgtccggc tcggcgaagc cctcgacggt
     1801 tcgcttgtcg ccggtgaact ggatggcggt catccggtag gcgctcgcca tcagcgcgta
     1861 ggcctcggcg tcgctgaacg agtcgaggtc ggtgcggatg gcggagagtt ggcgctggac
     1921 ggcgggcgcg acgccgaacg aggtcgagtc gttgcggccc gcgtcggcgc gctcgaagtc
     1981 cgactcgcgg tacttcgagg ggcagtcctt ccacgccaga ttctcgcccg tcaactcctg
     2041 ccgcaggtgg atgaacatca gcgcgcgcag cagcgacgag cggcggcgcg aggcgatgtc
     2101 cgtgtactgc gcctcgcgga tgcgcgcctg gaggatgctg ttggtgcgca gcggcgggcc
     2161 gagcagcccg ccgtcgggca cgttctgcgc gtccatctgc ccgctgccgt cgctgatgag
     2221 catcaccgtg cagtcctgtt cgagcaggct gttgatgccc tgattatcga cggcgccgcc
     2281 gtccacgagg cggacgctga tccgctgttt gtcgttgagc ttgccgtccg cgtcgtaggg
     2341 gtacagcccg tcgaggatga gcggctcgaa cagtccgggc acgcaggacg aggcggcgac
     2401 ggcgtggccg aggcggaact gtcggtacgg ctcgggcgcg ttctcgtagt acatccgccg
     2461 caggcggtag ttgcagtcga tgtcggcgtt gatgggcgtc ggcggctcgc ccatccacga
     2521 ggcggtgaac tgccagttgt ggcccgtgtt gagggtcgcc gcgttgagga tgaggtcggg
     2581 cgacttgctc gcgcgccgcc agttgtgcga gcgcggcttg aagtcggtct gctgcgtgcc
     2641 gtcgggcagc agcgggtgga tgtagaggtc gttgagccag cgccggtcgc gccgcagccc
     2701 gaagagcttc gccgcccagt cgggcagcca gcgcggcccc tcgaccttga cgccgtcggg
     2761 gacgcgcgcg aacagctcgc tctcgtacag ctcgccgacg cgcagcgtgc gcgagtagct
     2821 ggccgagaag atcatcttca ggttcgtcac ccactcggcc agcacgcgcg tgcggatgtt
     2881 gcgctgcacg cccttgagga attcgcgctc gatgcgctgg acgatcccga tgtagtcctc
     2941 gcgcgtgatc tcgtcgtcgc tct
//
LOCUS       DBNGOH010000303         2114 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2428968, whole genome shotgun sequence.
ACCESSION   DBNGOH010000303 DBNGOH010000000
VERSION     DBNGOH010000303.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2114)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2114)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2114
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2428968"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1050
                     /gene="metG"
                     /locus_tag="AC159P_05830"
     CDS             <1..1050
                     /gene="metG"
                     /locus_tag="AC159P_05830"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013567885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase subunit beta"
                     /protein_id="HIR3695008.1"
                     /translation="GVVPAPDISDERRLAAKRSGVDSDAMELASSLELARDQFVRHMD
                     EFAFNRALETAWGIIARVDKFITDAKPWVLAKSEEHRETLGAVLYRSAETIRWLAALL
                     YPFMPEASGAIARQLGIEDTLSDVDPSGLKWGDLKPGTKVGEVSPIFPRIDKAKTMAE
                     IEKTTASEREGDPQAAEQAGAKAAEQTGAHATQAAVPGAETATGTKPTVPPMKGATEA
                     DAVPAQTKEAGATKDESAGVTTLIGIEDFVKVELRAAQVLTAERVPKADKLLKLTVDL
                     GEAQPRQILAGIAQYYEPEKLVGRKIVVVANLQPRKMRGLESNGMLLAASVGDEGRPV
                     LATFTEDVPNGARLK"
     gene            1183..1977
                     /locus_tag="AC159P_05835"
     CDS             1183..1977
                     /locus_tag="AC159P_05835"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012974432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004536 - DNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HIR3695009.1"
                     /translation="MFVDSHAHIDGGEYDADRDEVVARARAAGVRAILNVGTGDPRSG
                     NFERAVAVAEKYEGVYAAVGVHPHDARLYDGEAERLLRSLVEGSERVIAWGEIGLDYH
                     YDHSPRDVQREVFVRQLRVAQEAGLPVVIHSREADEETVEILRAESSGGRLRGVMHCF
                     GGGQRMAEEVLGLGFMISFAGNVTFKKAGELRAVVLGVPPDRLLVETDCPYLAPVPLR
                     GRRNEPAYVVETARFLAELRGTSAEELGRVTAENFASFFGIDLAEG"
BASE COUNT          365 a          674 c          758 g          317 t
ORIGIN      
        1 ggcgtcgtcc ccgcgccgga catttcggac gagcgcaggc tcgcggccaa gcgctcgggc
       61 gtcgattcgg acgcgatgga gctggcgagc agcctcgaac tcgcgcgcga ccagttcgtg
      121 cgccacatgg acgagttcgc cttcaaccgc gcgctcgaaa cggcgtgggg catcatcgcg
      181 cgcgtggaca agttcatcac cgacgcgaag ccgtgggtgc tggcgaagtc cgaagagcac
      241 cgcgagacgc tcggcgccgt gctctatcgt tcggcggaga cgatccgctg gctcgccgcg
      301 ctgctctacc cgttcatgcc ggaggcttcg ggcgccatcg cgcgccagct cggcatcgaa
      361 gacacgctct cggacgttga cccttcgggg ttgaagtggg gcgacctgaa acccgggacg
      421 aaggtcggcg aggtgtcgcc catcttcccc cgcatcgaca aggcgaagac gatggctgaa
      481 atcgaaaaaa cgacagcgag cgagcgggaa ggcgacccgc aggcggcgga gcaggcgggc
      541 gcgaaagccg cggaacagac cggcgcgcac gccacgcagg ccgccgtgcc gggcgccgaa
      601 accgcgaccg ggacgaagcc caccgtgcct cccatgaagg gcgcgaccga ggccgacgcc
      661 gtccccgcgc aaacgaaaga ggcgggcgcg acgaaagacg agagcgccgg tgtcacgacg
      721 ctcatcggca tcgaggactt cgtgaaggtc gagctgcgtg cggcgcaagt gctgaccgcc
      781 gagcgcgtgc cgaaggccga caagctgctg aagctcaccg tcgatctcgg cgaggcgcag
      841 ccgcgacaga ttctcgcggg catcgcccaa tactacgaac ccgaaaaact ggtcgggcgc
      901 aagatcgtcg tcgtcgccaa cctccagccg cgcaagatgc gcggcctcga atccaacggc
      961 atgctcctcg cggcctccgt cggcgacgaa ggccgccccg tcctcgccac cttcaccgaa
     1021 gacgtgccca acggagcgag gctgaaatag taggcagtag gcagtaggca gtaggcagca
     1081 ggcaggtagt tgagcgagga cggcatagac gctggtgccc tgagctttcc atcgagaaat
     1141 gtttttactg cccgctgccc gctgcctact gcctactttc ttatgtttgt cgattcgcac
     1201 gcgcacatag acggcgggga gtacgacgcg gatcgcgacg aggtggtcgc gcgcgcgcgc
     1261 gcggcgggcg tgcgcgccat cttgaacgtg gggacgggcg accctcgttc ggggaatttc
     1321 gagcgggcgg tggcggtcgc ggagaagtac gagggcgtgt acgcggcggt cggcgtccac
     1381 ccgcacgacg cgcggctgta cgacggagag gcggagcggc tgttgcggtc gctggtcgaa
     1441 gggagcgagc gcgtcatcgc gtggggcgag atcgggcttg actatcatta cgaccactcg
     1501 ccgcgcgacg tgcagcgcga ggtcttcgtg cgccaactga gggtcgcgca agaggcgggg
     1561 ctgcccgtcg tcatccactc gcgcgaggcc gacgaagaga cggtcgagat actgcgtgcg
     1621 gaatcgtcgg gcgggcgctt gcgcggcgtc atgcactgct tcggcggcgg gcagaggatg
     1681 gcggaagagg tgctcggcct cggcttcatg atctcgttcg cgggcaacgt gactttcaag
     1741 aaggccggcg agctgcgcgc ggtggtgctc ggcgtgccgc ccgacaggct cctcgtcgaa
     1801 accgactgcc cgtacctcgc gcccgtgccg ttgcgcgggc ggcggaacga gccggcctac
     1861 gtcgtcgaga ccgcgcgctt tctcgcggag ctgcgcggca cgagcgcgga ggagttgggc
     1921 cgcgtcaccg ccgagaactt cgcaagcttt ttcggcatcg atctggcgga aggataatcg
     1981 gtgcttcgcg cctcgcgttg cgcacctacg ggtcgcccgc ggaagtaact ttgtctcgcc
     2041 gcgcgttaat gttataaagg ctgtgtccgg cgggcgggtt cggacgcgcg cttgatcttc
     2101 atcccggcgc cccg
//
LOCUS       DBNGOH010000304         8805 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_413026, whole genome shotgun sequence.
ACCESSION   DBNGOH010000304 DBNGOH010000000
VERSION     DBNGOH010000304.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8805)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8805)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8805
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_413026"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..298)
                     /locus_tag="AC159P_05840"
     CDS             complement(<1..298)
                     /locus_tag="AC159P_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013663838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HIR3695010.1"
                     /translation="MTTSKSRRKFIGSVGAAALVSGLAPESLAAGPGATNLGRQQQLR
                     PFGFIIHGGAGTIERSRMTPAREAAYRAKLSEALLAGYEVLRKDGAGLDAVVAAI"
     gene            complement(301..642)
                     /locus_tag="AC159P_05845"
     CDS             complement(301..642)
                     /locus_tag="AC159P_05845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695011.1"
                     /translation="MRQPTFFDKNEGDEGVDAERLVLYALGDFQARGLVLAERDLPLD
                     RLRGALRRASEAFDVPELSDERAAEAFTALGARVRRVPTYFAKHPFRVVVPEELARRA
                     SEVWRESRESE"
     gene            complement(774..1364)
                     /locus_tag="AC159P_05850"
     CDS             complement(774..1364)
                     /locus_tag="AC159P_05850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695012.1"
                     /translation="MKVGLFGRGCLALALCLVCTCVAAAQGSRRAQARASSEFGPIVQ
                     AYLDYLHTQQEVVDDRVSRREVDRRYYLHNSNRIRALRQVAIRIARENDNDYLPELEA
                     VSLDEFEQLFEEPLPEPTALRVGETLRYQFRFLEVVTVQREKFYVFARLDPYEQAELR
                     KQGEAKAKAEPRTEAAPTPPPATDSATRPRRTNSPE"
     gene            1688..2929
                     /gene="coaBC"
                     /locus_tag="AC159P_05855"
     CDS             1688..2929
                     /gene="coaBC"
                     /locus_tag="AC159P_05855"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020715625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HIR3695013.1"
                     /translation="MSTDVKTSFEGRRVALGVTGGIAAYKAAEVLRGLQRAGCAVRVG
                     MTKRACEFITPLTFRALSGAHVVVDDYAPDNPDPIAHITFSQTVDLFLVAPATANIIA
                     KFAGGVADDFISTTYLACNAPVVVAPAMNTTMWNHPATRRNLARLSADGVHIIQPDEG
                     EMACGTFGPGRLSDPGVIVAAALGVLEESLKRRAQDLAGERVLITTGATREEIDPVRF
                     ISNRSAGRMGFALAEAARARGAEVPVVAGSTSAEPPAGVRLVRALSAAEMHEAVMREV
                     PQASVFIAAAAVADYRPAVRAESKIKKSDAAMTLSLEPTMDILQAVAHTRRNGLLVVG
                     FAAESDNVLEHARLKLERKRLDAIVANDITQEGAGFDTETNAVTILTRDREQARSLPL
                     MSKLDAAHRVIDEIARLRRGL"
     gene            2988..4040
                     /locus_tag="AC159P_05860"
     CDS             2988..4040
                     /locus_tag="AC159P_05860"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HIR3695014.1"
                     /translation="MRDELRALAADVTESASYLRELGVEVLEGFEASVVGEASKSSPA
                     VAPHSERRAPLPPALERNARAERVAPPKMPAAAPPPKPTIAPLPKMPAATPPSQTRVE
                     PPPSKMTSVGAAPRAGAAPPTLDQTAMPKKPVRAPAANPPEPRETLFGDIAPKDELSL
                     GVPGETFEDIREDVGECVRCPLHQGRTKIVHTEGQPTARLMFVGEAPGADEDASGRPF
                     VGRAGQLLNKIIEAIGLKREDVLIGNVNRCRPPGNRQPTTEEAKTCKPFLLREINIAR
                     PAVIVVLGNTAMKNLLDTKEGITRLRGQFQDYKGIKVMPTFHPAYLLRDPSKKREVWA
                     DLKKVRDYLNQKKSEA"
     gene            4108..4704
                     /locus_tag="AC159P_05865"
     CDS             4108..4704
                     /locus_tag="AC159P_05865"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HIR3695015.1"
                     /translation="MQDSLFLDAPQPASDETPDAVNEELLRRAKSLPHFKERVFIFGE
                     GPRGARVAVVGESPGPPDIESGRPFMGPAGQMLERILGSIGLARADCYLTNVIKVISQ
                     GEEITADWLALFTPYLHRELAAVRPRVIISFGNTPTRALLRTKKPISQLRGEFHDYDG
                     VRLMPTFNPAYLLRDPTKKREVWEDMKKVRALLAGLKD"
     gene            4803..7382
                     /gene="priA"
                     /locus_tag="AC159P_05870"
     CDS             4803..7382
                     /gene="priA"
                     /locus_tag="AC159P_05870"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF004066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HIR3695016.1"
                     /translation="MHSSAVSPTDDTETRAGRDAALYAEVVFPLRLAQTFTYRLPVAL
                     REEARVGSRLLVPFGRKMATAYVVELHDALDPAAELDETEIREAEELLDAEPLLTPEV
                     LEITRWLSDYYAAPWGEVLRAALPAGLNSTIEQVIRVTSEGRDELARLPARRAATTRA
                     QVLSFVAHGGEVTLREVAREQGQARAAKAVRELERAGWVTVTHRAKGALAKTKRRKAV
                     RLLSHETQDVNGAGATQDVDEGRAVQDANGARALTAAQRRVLEVLAENGGEMLFAELL
                     DAAHAGVSSVQTLARRGLLEVFVRDVRRDPLAGAALPSADDFRLTSEQASALKEINEA
                     QAAGGYAAFLLHGVTGSGKTEIYIRAMRAALERGRSAMMLVPEIALTPVFSRRLRAHF
                     GERVAIFHSSLTTGERFDEWGRLKRGEARVVIGTRSAVFAPVSDLGLVVVDEEHEASY
                     RQQESPYYNGRDTAVMRALKERAVVVLGSATPSLESFQNARRGKYRYLRLPNRVAGRA
                     MARAEIIDMREVFGTSGKPEVFSPALLAAIEETHARGEQSIVLLNRRGYSSFVLCRTC
                     GERINCRNCDVTLTYHRTERSLICHYCNLRERVPAVCPACKGKYIFFIGEGTEQVEEI
                     LRRRFPSLRIARLDRDTTARRSTYEHAILSFGAGELDMLVGTQMIAKGHDFPNVTLVG
                     VISVDAGLALPDFRASERAFQLITQVAGRAGRGERPGKVLIQTYHPHHYALRHACAQD
                     YETFFEEEIRYRKTLSYPPFVALASLLVHGEDLVRAQATADEIRRALDAANNDRAARV
                     LGPAPAPLSRLRGEHRVQILLKSRNRARMRELIELALADAARVPGCDVQSVNVEIDPV
                     NLM"
     gene            7418..8497
                     /gene="lpxD"
                     /locus_tag="AC159P_05875"
     CDS             7418..8497
                     /gene="lpxD"
                     /locus_tag="AC159P_05875"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HIR3695017.1"
                     /translation="MKLTELARLTGAQLEGDFPDVEIHGAAGLDEAETGHVTFLSNPR
                     YTPRVQTTRASAVYLGEGVEPGRADLAVLRARDPYLAYTRALRLFHPEPDFEPFIHPA
                     ASVDPSARVGEGVYVGACSVVGRNAEIGDGVYIHPNVTVYDNVRVGARSVIHSGVALR
                     EGTRVGERVRIHNNTVVGSDGFGYAKDEEGRWLKIPQAGRVVIEDDVEIGAGTTIDRA
                     SVGETRIRRGAKIDNLVQVGHSCTVGEDALLCAQVGLAGSSHIGRRVILAGQVGVAGH
                     LTVGDDVVITAKSATSHNVPDGKILSGIPAFDNRDWLRATAAFRRLGDMQRALRTLET
                     RLAALEKHSPQDESPETDSPPGEQP"
BASE COUNT         1495 a         3180 c         2769 g         1361 t
ORIGIN      
        1 tgatggcggc gacgacggcg tccagccccg cgccgtcctt ccgcagaact tcgtaaccgg
       61 cgagcagcgc ctcggagagt ttcgcgcggt aggcggcctc gcgcgcgggc gtcatgcggc
      121 tccgctcgat ggtgcccgcg ccgccgtgga tgatgaagcc gaaggggcgg agttgttgtt
      181 gtcgtcccaa gttcgtcgcc cccggccccg cggcgagcga ctccggcgcg aggcccgaga
      241 cgagcgcggc ggccccgacg ctcccgatga attttcggcg gctcttactc gtagtcataa
      301 ctattcggac tccctcgact cgcgccagac ctcgctcgcg cgccgcgcga gttcttcggg
      361 gacgacgacg cggaacgggt gtttggcgaa gtaggtggga acgcggcgca cacgcgcgcc
      421 gagtgcggtg aacgcctccg cggcgcgctc gtcgctgagt tccggcacgt cgaaggcttc
      481 cgacgcgcga cgcagcgccc cgcgcagacg atccagcggc aggtctctct cggcgagcac
      541 cagcccccgc gcctggaagt cgccgagcgc gtacagcacg agccgctcgg catcaacccc
      601 ttcgtcgccc tcgttcttgt cgaagaatgt cggctggcgc atcaattgat ttcggatgtc
      661 ggatgtcgga tttcggattg aagacggatg attgacgcga gcagcgccat catcaaccgt
      721 caggcgttaa gagacatcaa tccgaaatcc gaaatctaaa atggtctcac tgctcactcg
      781 ggagagttgg tgcggcgcgg gcgcgtggcc gagtcggtcg cgggcggcgg ggtcggcgcg
      841 gcctcggtgc gcggctcggc tttggccttt gcctcgccct gcttgcgcag ctcggcctgc
      901 tcgtaggggt cgagccgcgc gaagacgtag aacttctcgc gctgcacggt cacgacttcg
      961 agaaacctga actggtagcg gagcgtctcg ccgacgcgca gggccgtcgg ctccggcagc
     1021 ggctcttcga aaagctgctc gaactcgtcg agcgagacgg cttcgagttc gggcaggtag
     1081 tcgttgtcgt tctcgcgcgc gatcctgatg gccacctgcc ggagcgcgcg gatgcggttc
     1141 gagttgtgca ggtagtagcg gcggtcaacc tcgcggcggc tgacgcggtc gtccaccacc
     1201 tcctgctgcg tgtgcaggta atcgaggtaa gcctgcacga tggggccgaa ctcggaggag
     1261 gcgcgcgcct gtgccctccg cgagccttgc gccgcggcga cgcaggtgca gacgaggcac
     1321 agcgccaacg ccagacaccc gcgcccgaac agaccgactt tcaccctcga caccttattc
     1381 acctcggaca ctatcgcgcg acgccgcttc cgcatttcgt cgggatattt gcacgggagt
     1441 ttaccacaga agcgagcggg cggtgagcag tagcgggcaa gccggcagcg ggcagcaggc
     1501 agcaggcagc aggagggaga tgtccgggac tttacctccc aagcctttgc gttcagcaag
     1561 cagtgaacgg cggaagcttg agaggtaaag tcccgagcct ccttcggcct tccgtcttat
     1621 ctgcccgctg cctgctgcct actgcctact gtttgtgata aattcgcgcg cgggcggtga
     1681 gctttctatg tcaactgacg tgaagacgag cttcgagggc cggcgcgtgg cgctgggcgt
     1741 gacgggcggc atcgccgcgt acaaggccgc ggaggtcttg cgcggcctcc agcgcgcggg
     1801 ctgcgcggtg cgcgtcggga tgacgaagcg cgcgtgcgag ttcatcacgc cgctgacctt
     1861 ccgcgcgctc tccggcgcgc acgtcgtcgt ggacgactac gcgcccgaca acccagaccc
     1921 tatcgcgcac atcaccttct cgcagaccgt cgatctcttc ctcgtcgcgc ccgccaccgc
     1981 caacatcatc gccaagttcg cgggcggcgt ggccgacgat tttatctcga ccacatacct
     2041 cgcgtgcaac gccccggtcg tcgtcgcacc ggcgatgaac acgacgatgt ggaaccaccc
     2101 ggcgacgcgc cgcaacctcg ctcgcttgag cgcggacggc gtgcatatca tccagccgga
     2161 cgagggcgag atggcctgcg gcacgttcgg ccccggtcgt ttgagcgacc ccggcgtcat
     2221 cgtcgccgcg gcgctcgggg tgctcgaaga gtctctgaag cgccgcgcgc aagacctcgc
     2281 gggcgagcgc gtcctcatca cgaccggcgc cacgcgcgaa gagatcgacc ccgtgcgctt
     2341 catttcgaac cgctctgccg gcaggatggg cttcgcgctg gccgaggccg cgcgcgcgcg
     2401 cggcgcggag gtcccggtcg tcgccggctc gacgagcgcc gagccgcccg cgggcgttcg
     2461 cctcgtccgc gcgctctccg cggcagagat gcacgaggcc gtcatgcgcg aggtgccgca
     2521 agccagcgtc ttcatcgccg ccgccgcggt cgccgactac cggcccgccg tgcgcgccga
     2581 gagcaagatc aagaagtccg acgcggccat gacgctctcg ctcgaaccga ccatggacat
     2641 ccttcaggcg gtcgcgcaca cacgccgcaa cggcctgctc gtcgtcggct tcgccgccga
     2701 gagcgacaac gtcctggagc acgcgcgcct caagctcgaa cgcaaacgcc tcgacgccat
     2761 cgtcgccaac gacatcacgc aggagggcgc cggcttcgac acggagacca acgccgtcac
     2821 catcctcacg cgcgaccgcg aacaggcgcg gagtctcccg ctcatgtcga agctcgacgc
     2881 cgcgcaccgc gtcatcgacg agatcgcgcg cctccgccgc ggcctttaaa ttccgcgccc
     2941 gcttcgtgcg ataatgcttt caccgtacga tggaagagca gaaggacttg agagacgaac
     3001 tgcgggcgct ggcggcggac gtgaccgaat cggcctcgta cctgcgcgag ctgggcgtcg
     3061 aagtgctcga agggttcgag gcatcggtgg tcggtgaggc ttcgaaaagt agcccggcgg
     3121 tcgcgccgca ttcggagcga cgcgcgccgc tcccgcccgc gctcgaacgc aacgcgcgtg
     3181 cggagcgggt cgcgccgccg aagatgcccg ccgcggctcc cccgccgaag ccgacaatcg
     3241 cgccgctgcc gaagatgccc gcggcgactc ccccttcaca gactcgtgtc gaaccgcccc
     3301 cttcgaagat gacctcagtc ggcgcggcgc ctcgcgcggg cgcggcgccg ccaactctcg
     3361 atcagacagc gatgcccaag aaacctgtcc gtgcaccagc cgccaatccg cccgaaccgc
     3421 gcgagacgct gttcggcgac atcgcgccga aggacgagct gagcctcggc gtccccggcg
     3481 agaccttcga ggacatacgc gaagacgtgg gcgagtgcgt ccgctgtcct cttcatcagg
     3541 ggcgcacgaa gatcgtccac accgaaggcc agccgaccgc tcgtctgatg ttcgtcggcg
     3601 aggcgccggg cgcggacgag gacgcttccg gaagaccctt cgtcggtcgc gcgggccagc
     3661 tcctcaacaa gatcatcgag gccatcggcc tcaagcgcga ggacgtgctc atcggcaacg
     3721 tcaaccgctg ccgcccgccc ggcaaccgcc agccgacgac cgaagaggcg aagacctgca
     3781 aacccttcct cctgcgcgaa atcaacatcg cgcgccccgc cgtgatcgtc gtcctcggca
     3841 acaccgcgat gaagaatctg ctcgacacga aggaaggcat cacgcgcctg cgcggccagt
     3901 tccaggacta caagggcatc aaggtcatgc cgaccttcca ccccgcttac cttctgcgcg
     3961 acccctcgaa aaagcgcgaa gtgtgggccg atttgaagaa ggtacgcgac tacctcaacc
     4021 agaagaagtc agaagcctga agtcagaagt cagaacaaaa agcatttgtc tctgacttct
     4081 gacttctgac ttctgacttg aattattatg caagactctc tcttcctcga cgcgccgcag
     4141 cccgcatccg acgagacgcc cgacgccgtc aacgaagagc tgctgcgccg cgcgaagagc
     4201 ctgccgcact tcaaggagcg cgtcttcatc ttcggcgagg ggccgcgggg ggcgcgcgtc
     4261 gcggtcgtcg gcgaatctcc cggcccgccc gacatcgaga gcggcaggcc cttcatgggg
     4321 cctgcgggcc agatgctcga acgcatcctc ggcagcatcg gcctcgcccg cgccgactgt
     4381 tacttgacca acgtgataaa agtcatcagc cagggcgagg agatcaccgc tgactggctc
     4441 gccctcttca cgccttatct gcaccgcgag ctggccgccg tccgcccgcg cgtcatcatc
     4501 tccttcggca acaccccgac gcgcgcactg ctccgcacca aaaagcccat ctcccaactg
     4561 cgcggcgagt tccacgacta cgacggcgtc cgactgatgc cgaccttcaa ccccgcgtat
     4621 cttttgcgcg acccgaccaa gaagcgcgaa gtctgggagg acatgaaaaa ggtgcgcgcc
     4681 cttctcgccg ggttgaaaga ttagacgcgc actacattcg actgcgactt gctcgtcatt
     4741 ccgcgccgac tttcattccg gtcacttccc cttttaccat cttcacgcta acttagtccg
     4801 ccgtgcattc ttccgccgtc tcaccgaccg acgacaccga aacgcgcgcg gggcgcgatg
     4861 ccgcgctcta cgccgaagtc gtcttcccct tgcgcctcgc gcagaccttc acgtaccggc
     4921 tgccggtcgc cctgcgcgag gaggcgcgcg tcggctcgcg cctcctcgtg cctttcggga
     4981 ggaagatggc aacggcctac gtcgtcgagc tgcacgacgc gctcgacccc gcggcggagc
     5041 tggacgagac ggaaatcaga gaggccgagg agctgctcga cgcggagcct ttgctcacgc
     5101 cggaggtctt ggagatcacg cgctggctca gcgactacta cgccgcgccg tggggcgagg
     5161 tgcttcgcgc ggcgctgccc gccggcctca actcgaccat cgaacaggtc atccgcgtca
     5221 cctcggaagg gcgcgacgaa ctcgcgcgcc tgcccgcgcg gcgcgccgcg acgacgaggg
     5281 cgcaagtcct ctccttcgtc gcgcacggcg gcgaggtcac gctgcgcgag gtcgcacgcg
     5341 agcagggtca ggcgcgcgcc gcgaaggcgg tgcgcgagct tgagcgcgcg ggctgggtca
     5401 cggtcacgca ccgcgcgaag ggcgcgctgg cgaagaccaa gcggcgcaag gccgtccgcc
     5461 tgctctcgca cgaaactcaa gacgtgaacg gcgcaggcgc gacgcaagac gttgatgaag
     5521 gtcgtgctgt tcaagacgcg aacggcgcgc gcgcgttgac ggcggcgcag cggagggtct
     5581 tggaagtgct cgcggagaac ggcggcgaga tgctcttcgc cgagctgctc gacgcggcgc
     5641 acgcgggcgt ctcctcggtg cagacgctcg cgcgccgtgg gctgctcgaa gtcttcgtcc
     5701 gcgacgtgcg gcgcgacccg ctcgcgggcg ccgcgctccc ctcggcggac gattttcgct
     5761 taacgagcga acaggcgtcg gcgctcaaag agattaacga ggcgcaggcg gcgggcggtt
     5821 acgcggcctt cctcctgcac ggcgtgacgg ggagcgggaa gaccgaaatc tacatccgcg
     5881 cgatgcgtgc cgcgctcgaa cgcgggcgct cggcgatgat gctcgtgccc gaaatcgctc
     5941 tcacgccggt cttctcccgc cgcctgcgcg cgcacttcgg cgagcgcgtc gccatcttcc
     6001 actcgtcgct caccacgggc gaacgcttcg acgagtgggg ccgcctcaag cgaggcgagg
     6061 cgcgcgtcgt catcggcacg cgctcggcgg tcttcgcgcc cgtctcagac ctcggcctcg
     6121 tcgtcgtgga cgaagagcac gaagcctctt atcgtcaaca ggagtcgccc tactacaacg
     6181 ggcgcgacac cgccgtcatg cgcgctttga aggagcgcgc cgtcgtcgtc ctcggctccg
     6241 cgacgccctc gctcgaatcc ttccagaacg cgcgccgcgg caagtaccgc tacctccgac
     6301 tgccgaaccg cgtggcgggc cgcgcgatgg cgcgcgccga gatcatcgac atgcgcgagg
     6361 tcttcggcac gtcgggcaag cccgaagtct tctcgcccgc gctgctcgcc gccatcgagg
     6421 agacgcacgc gcgcggcgag cagtccatcg tgctcctcaa ccgccgcggc tactcgtcgt
     6481 tcgtgctgtg ccgcacctgc ggcgagcgca tcaactgccg gaactgcgac gtgacgctca
     6541 cctaccaccg cacggagcgc agcctcatct gccactactg caacctgcgc gagcgcgtcc
     6601 ccgcggtctg ccccgcctgc aagggcaagt acatcttctt catcggcgag ggcacggagc
     6661 aggtcgaaga gatattgcgc cggcgcttcc cttccctgcg catcgcgcgc ctcgaccgcg
     6721 acacgaccgc gcgccgctcg acttacgagc acgccatcct cagcttcggc gcgggcgaac
     6781 tcgacatgct cgtcggcacg cagatgatcg ccaaagggca cgacttcccc aacgtcacgc
     6841 tcgtcggcgt catctccgtc gatgccggtc tcgcgctccc cgacttccgc gcctccgaac
     6901 gcgccttcca gctcatcacg caggtcgccg gtcgcgcagg ccgcggcgag cgccccggca
     6961 aggtcttgat tcagacctac cacccgcacc actacgccct gcgccacgcc tgcgcgcagg
     7021 actacgagac ttttttcgag gaggagattc gctaccgcaa gactttgagc tacccgccct
     7081 tcgtcgcgct cgcctcgctg ctcgtccacg gcgaagacct cgtgcgcgcg caagcgaccg
     7141 cggacgaaat ccgccgcgcc ctcgacgccg ccaacaacga ccgcgccgcc cgcgtcctcg
     7201 gccccgcgcc cgccccgctc tcgcgtctgc gcggcgaaca ccgcgtgcag attctcctca
     7261 agtcgcgcaa ccgcgcgcgc atgcgcgaac tcatcgagct ggcgctcgcc gacgccgcac
     7321 gcgtccccgg ctgcgacgtg caatccgtca acgtcgagat cgaccctgtt aatctcatgt
     7381 gacctgtcat tcgttttcga acaccggcat tcccatcatg aaacttaccg aactcgcccg
     7441 cctgacgggc gcgcaactcg aaggcgactt ccccgacgtt gaaatccacg gcgcggcggg
     7501 actcgacgaa gccgagaccg gccacgtcac cttcctctcc aacccgcgct acacgccgcg
     7561 cgtgcagacg acgcgcgcct ccgccgtcta cctcggcgaa ggcgtcgagc cgggccgcgc
     7621 cgacctcgcc gtgctgcgcg cccgcgaccc ttacctcgcc tacacgcgcg cgctgcgcct
     7681 ctttcatccc gagccggact tcgagccgtt catacacccc gcggcctccg tcgatccttc
     7741 ggcgcgcgtc ggcgaaggcg tctacgtcgg cgcgtgctcc gtcgtcggtc gcaacgccga
     7801 gatcggcgac ggcgtctaca tccacccgaa cgtcaccgtc tacgacaacg tccgcgtcgg
     7861 cgcgcgctcc gtcatccact cgggcgtcgc cctgcgcgag ggcacccggg tcggcgagcg
     7921 cgtccgcatc cacaacaaca cggtcgtcgg ctcggacggc ttcggctacg cgaaggacga
     7981 ggagggccgc tggctgaaga ttccgcaggc gggccgcgtc gtcatcgaag acgacgtgga
     8041 gatcggcgcc ggcaccacca tcgaccgcgc ctcggtcggc gagacgcgca tccgccgcgg
     8101 cgccaagatc gacaacctcg tgcaggtcgg ccactcctgc acggtcggcg aagacgctct
     8161 gctctgcgcg caggtcggcc tcgccggaag ctcgcacatc gggcggcgcg tcatcctcgc
     8221 gggccaggtc ggcgtcgccg gccacctcac cgtcggcgac gacgtggtca tcacggccaa
     8281 gagcgccacc agccacaacg tccccgacgg caaaatcctc tccggcatcc ccgccttcga
     8341 caaccgcgac tggctccgcg ccaccgccgc cttccgccgc ctcggagaca tgcaacgggc
     8401 gctgcgcaca ctcgaaacgc gcctcgccgc gctcgaaaaa cactcacccc aggatgaatc
     8461 gcccgaaacc gactccccgc ccggcgaaca gccttgaaaa ctccgcgccg cgtggcgagt
     8521 cataaaaaaa tcaccacgcg gccaacaatc ttcttgacag ccgggcgcct cacgaagata
     8581 atgcttgttg aaattggatt tcaatttcgc tccatcgcga gcgaaactga aatcgggatt
     8641 tccaagtttc cgaaggagct ttgaacggcg gtgcagataa ccaacaacgc ggcgtctcac
     8701 gtcatcgaga ccgcgcgcga agaccaaaaa gtagaagtcg tcatcgaggg cgccgaccgc
     8761 gtggcggtgc gctatctgac ctggacggaa ggtctcggct ggtgt
//
LOCUS       DBNGOH010000305         2175 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2429356, whole genome shotgun sequence.
ACCESSION   DBNGOH010000305 DBNGOH010000000
VERSION     DBNGOH010000305.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2175)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2175)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2175
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2429356"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..862)
                     /locus_tag="AC159P_05880"
     CDS             complement(<1..862)
                     /locus_tag="AC159P_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011984811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695018.1"
                     /translation="MKDDAEPINPSEVAPGSRGEGSRAERLTAARIVWLAKAAALTLA
                     VTMALLFVATPTGSKILKFLGFDKEIEQNAARMLKEGRQTFRSDTFGDEAFWGDLLKL
                     HLAIEGAQFGGVGPGLSPRAALGLGLKVDVDALPQGLVKQLRQGRVNLDDPAVTLALL
                     RHNAVLGVKGFFGDDDGLQSVGITCALCHSVVDDSLAPGIGRRLDGWANRDLNVGEVV
                     ALAPDLGPFSSLLGVGQDTVRAVLRSWGPGKFDAELILDGKAFNPQQVSNGVVTGTNV
                     SGATLLPPAFG"
     gene            1112..1723
                     /locus_tag="AC159P_05885"
     CDS             1112..1723
                     /locus_tag="AC159P_05885"
                     /inference="COORDINATES: protein motif:HMM:NF013209.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HIR3695019.1"
                     /translation="MSVPDDREGLRFPCECVSGRGGGYSDPWTAVAKNKLLPNGTKEV
                     ILNLLTREPKTISQLAAELSLSAPSVYAHVNDMLRSELLRESEEWEKKHPSERYYEPN
                     FPVFRAEECAEFEAVCQEMAEQLVALFEKKRPQMERAFDKTGFASRGWELRDVTQCLY
                     ANMQRKARALLEERGLLSPPEKHANGVEWVFWAEEPKADANEK"
     gene            complement(1741..>2175)
                     /locus_tag="AC159P_05890"
     CDS             complement(1741..>2175)
                     /locus_tag="AC159P_05890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695020.1"
                     /translation="ALIENWDLKDSNNRVLAVSGAGGGELHYIVSDLGATFGKTGGQS
                     SPMAFLRNVKGSRNEPEDYASDKFIEGIEGHQVRLHFSGKNSGLMRGVTVEDAKWLGA
                     ILSRLSDRQIEDAFRAANYGPDEVRLLSRAVRSRIGELTGLR"
BASE COUNT          385 a          704 c          705 g          381 t
ORIGIN      
        1 gaccgaaggc gggcgggagc agcgtggcgc ccgacacgtt cgtcccggtc acgacgccgt
       61 tggagacttg ctgggggtta aatgccttgc cgtccaggat cagctcggcg tcgaatttcc
      121 ccggccccca actcctgagc accgcccgca cggtgtcctg gccgacgccc aagaggctgc
      181 tgaacgggcc gaggtcgggg gcgagcgcga cgacctcgcc gacgttgagg tcgcggttgg
      241 cccacccgtc gaggcggcgg ccgatgccgg gcgcgagcga gtcgtccacc accgagtggc
      301 agagggcgca agtgatcccg acggactgta agccgtcgtc atcgccgaag aatcctttca
      361 cgcccaagac cgcgttgtgc ctgagcaggg ccagcgtgac ggcggggtcg tcgaggttga
      421 cccgcccctg tctcaactgc ttcacgaggc cctgcgggag ggcatccaca tcgaccttga
      481 gacccaagcc gagcgcggcc cgcggactca gccccggccc cacgcccccg aactgtgcgc
      541 cttcaatcgc cagatgcagc ttgagcagat cgccccagaa tgcctcgtcg ccgaaggtgt
      601 cggagcggaa cgtctgcctg ccctctttga gcatccgcgc ggcgttctgc tcaatctctt
      661 tgtcgaaacc gaggaatttc aaaattttgg agccggtagg agtggcgacg aagagcagcg
      721 ccatcgtgac ggccaacgtc agcgccgccg ccttggccaa ccatacgatc cgtgccgcgg
      781 tgagcctctc ggcgcggctg ccttcacctc tgcttccggg cgcgacttcc gacggattga
      841 tcggctccgc gtcgtctttc atctttcttt acctcctctg gttaaaaatc gggacagacc
      901 gcagggtgta tctttcgtgc tcggggcgcg aggcggaatc catggggcgc cgggtctcac
      961 ggctttgcat aaatgacacg gtcgatgtta cgttccaccg cgaacatata cggagacatt
     1021 ttgcgcccct cggctgagaa gccgggggtg tttcgttagg ggataggctt ttaggatacg
     1081 ccctaaaagc gaatctgtaa aggggtgtct gatgagcgtg cctgatgatc gtgaagggtt
     1141 acggttcccc tgcgagtgcg tgtcggggcg gggcggcggc tacagcgacc cgtggacggc
     1201 ggtcgctaaa aacaagctcc tgcccaacgg cacgaaggaa gtgattctga acctcctgac
     1261 tcgcgagccg aagaccatct cccagctcgc cgcggagctt agcctttcgg cgccgagcgt
     1321 ttacgcgcac gtcaacgaca tgctgcggag cgagctgctg cgcgaatccg aagagtggga
     1381 gaagaagcac ccgtccgagc gttattacga gccgaacttc ccggtcttca gggccgagga
     1441 gtgcgccgag ttcgaggccg tttgtcagga gatggccgaa cagttggtcg ccctcttcga
     1501 gaagaagcgc ccgcagatgg aacgcgcctt cgataagacc ggcttcgcga gtcgcgggtg
     1561 ggaactacgg gacgtgaccc agtgcctgta cgccaacatg cagcgcaaag cgcgcgccct
     1621 gcttgaggag cgcggactac tctccccgcc cgagaagcac gccaacggcg tggagtgggt
     1681 cttctgggcg gaggaaccca aagccgacgc gaacgaaaaa tgaggccgcg tcaacgcacg
     1741 ttaccgcagc ccggtcagct cgccgattcg tgagcgcacg gcccgcgaga gcagacgcac
     1801 ctcgtcgggg ccgtagttgg ccgcgcggaa cgcgtcctcg atctgccggt cggagaggcg
     1861 cgagaggatc gccccgagcc acttcgcgtc ctcgaccgtg acgccgcgca tcagcccgct
     1921 gttcttgccg ctgaagtgga gccgcacctg atggccttcg atgccctcga tgaacttgtc
     1981 cgaggcgtag tcctccggct cgttgcggct ccccttgacg ttgcgcagga aagccatcgg
     2041 cgaactctgc ccgcccgtct tcccgaaggt cgcgccgagg tcgctgacga tgtagtgcag
     2101 ctcgccgccg cccgccccgc tcaccgccag cacgcggttg ttcgagtcct tgaggtccca
     2161 gttctcgatg agcgc
//
LOCUS       DBNGOH010000306         3068 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3345854, whole genome shotgun sequence.
ACCESSION   DBNGOH010000306 DBNGOH010000000
VERSION     DBNGOH010000306.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3068)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3068)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3068
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3345854"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            54..377
                     /locus_tag="AC159P_05895"
     CDS             54..377
                     /locus_tag="AC159P_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007933527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HIR3695021.1"
                     /translation="MEALGMVECRGLVAMIEAADAMVKAANVTLVGYEKIDAGLVTAI
                     VRGEVGAVRAAVDAGAAAGRRVGEVVATHVIPRPHAEVEDGLPVVKTGETTRKKISGS
                     VRGKK"
     gene            complement(611..2932)
                     /locus_tag="AC159P_05900"
     CDS             complement(611..2932)
                     /locus_tag="AC159P_05900"
                     /inference="COORDINATES: protein motif:HMM:NF012471.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HIR3695022.1"
                     /translation="MPRRLLPFALLAAALLSPLLALAPGAHARLAQSVPAPKDVLGFE
                     PGDDRKLADWSAIVDYFRKLDAASDRVKVEELGKTTMGAPFVVATISAPENLARLDEF
                     REIQRQLADPRTLGPQADRKVAALIARGKTVVLITCGIHSTEVGSTLSSTLIAHRLAA
                     SDEPEIRDVLKNTIILLVPSLNPDGVDIVKRWHDRVLGTPHEGTSPPELYHKYVGHDN
                     NRDWYAFTQAETRLTVDKLHNVWRPQIVHDIHQQGQFGSRLFLPPYMKPVEPNVPRQI
                     VEGYTELGLSMSKNLLAKGFKGITWDSTYDAWTPARAYSHYHGGVRILSETASANLST
                     PVNLRFEQLRSREGYDPQKASANFPEPWRGGEWRLRDITRYMTAAAFELLTHASQNRE
                     RWLRRFYEVGKEAVRPRRPGELFAWIIPPSENSTALIVTLERSGVETTTVDRMTVGGV
                     AYPAGTTVVRMDQPYGGFAKALLETQRYPDLRDSAGHPIPPYDVTAHTLPLLMGVEAK
                     AVFQPFKYRERLVGGRSENRGGSRPRLALYKSHVPSMDEGWTRWVFEQNDFKYDSLLD
                     QEVRAGDLRAKYDAIVLPDQSPRALLEGHRKGAMPDELTGGLGAEGVKALREFVVAGG
                     TLITLNQASQFAVEQFQLPLRDVTEGLRRTEFYAPGSIMRTILDTTHPIAKDMPRESI
                     AWFEDSPVFEIKSDPVALPRVKIIARYPQTGTPLLSGWLLGEERMRGRAALVEANLGE
                     GRIFLFGFRPQYRAQSLATYPLLFNAIMSARQQ"
BASE COUNT          503 a          896 c         1129 g          540 t
ORIGIN      
        1 atctgaaatc tcaaagctga gtttcttaac tgtcttttcg ggaggtgtga gggatggaag
       61 cgttggggat ggtcgaatgc cggggcctcg tggcgatgat cgaggcggcg gacgcgatgg
      121 tgaaggcggc caacgtcacg ctcgtcggct acgagaagat cgacgcgggg ctggtgacgg
      181 ccatcgtgcg cggcgaggtc ggggcggtgc gcgcggcggt ggacgcgggc gcggcggcgg
      241 gccgaagggt cggcgaggtg gtggccacgc acgtcatccc gcgcccgcac gcggaggtcg
      301 aagacggcct gcccgtggtc aagaccggcg agacgacgcg caagaagatt tccggctcgg
      361 tcaggggaaa gaagtgatga ggactgagga ctgagtgaga tggcgcgccc ttcggtagaa
      421 gtcgagaccg ccgccggcga aggcgcttcg gacgccgttt cgccggctac ttcgtcatct
      481 acgtcttcat ctatctcgcc tcgctcttcg tgacgctgct cacgttggcg agaggataaa
      541 gaaggcgaaa gtgaaaaggc aaaagtcgat gacgagaggc acgctgcctc ttatcttaac
      601 ttttgccttt ttactgttgc cttgccgaca tgatggcgtt gaagaggagc gggtaggtgg
      661 cgagcgactg cgcgcggtat tggggacgga agccgaagag gaagatgcgg ccctcgccga
      721 ggttcgcttc gacgagcgcg gcgcgaccgc gcatcctctc ttcgccgagc agccagccgg
      781 agaggagcgg cgtccccgtc tgcgggtagc gcgcgatgat tttgacgcgc ggcagggcga
      841 cggggtcgct cttgatctcg aagacgggcg agtcctcgaa ccacgcgatc gattcgcgcg
      901 gcatgtcttt ggcaatcggg tgcgtcgtgt cgaggatggt gcgcatgatc gagccgggcg
      961 cgtagaactc cgtgcgccgc aagccttcgg tcacgtcgcg caaggggagt tggaactgct
     1021 cgacggcgaa ctgcgaagct tggttcagcg tgatgagcgt gccgcccgcg acgacgaact
     1081 cgcgcagcgc cttgacgccc tccgccccga gtccgcccgt cagctcgtcc ggcatcgcgc
     1141 ccttgcggtg gccttcgagc agcgcgcgcg gcgactggtc ggggaggacg atggcgtcgt
     1201 acttcgcgcg caggtcgccc gcgcgaactt cttggtcgag tagcgagtcg tacttgaagt
     1261 cgttctgctc gaagacccag cgcgtccagc cctcgtccat cgaagggaca tgcgacttgt
     1321 agagcgcgag ccgggggcgg gagccgccgc ggttctcact cctgccgccg acgagccgct
     1381 cacgatactt gaacggctgg aacacggctt tggcctcgac gcccatcaac agcggcagcg
     1441 tgtgcgccgt gacatcgtaa ggcgggatgg ggtggcccgc cgagtcgcgc aggtcggggt
     1501 agcgctgcgt ctccaacagc gccttcgcaa agccgccgta cggctgatcc atgcggacga
     1561 cggtcgtgcc tgccgggtag gcgactccgc cgaccgtcat cctgtccacc gtcgtcgttt
     1621 cgacgccgct ccgctccaaa gtgacgatca gggcggtgct gttctccgac ggcggaatga
     1681 tccacgcgaa caactcgccg ggccgtcgcg ggcgcacggc ctccttcccg acttcgtaga
     1741 agcgccggag ccagcgctcg cggttctgcg aggcgtgggt caaaagctcg aaggcggcgg
     1801 cggtcatgta gcgcgtgatg tcgcgcaggc gccactcgcc gccgcgccac ggctccggga
     1861 agttggccga agctttttgc gggtcgtagc cttcgcgtga gcggagctgc tcgaagcgga
     1921 ggttgacggg cgtggagagg ttggccgagg ccgtctcgga gaggatgcgg acgccgccgt
     1981 ggtagtgcga gtaggcgcgc gccggagtcc acgcgtcgta ggtcgaatcc caggtgatgc
     2041 ctttgaagcc tttggcgagc aggttcttcg acatcgagag gccgagttcg gtgtagccct
     2101 cgacgatttg gcgggggacg ttcggctcga cgggcttcat gtagggcggc aggaagaggc
     2161 gcgagccgaa ctggccctgc tggtgtatgt cgtggacgat ctgcgggcgc cagacgttgt
     2221 gcagcttgtc cacggtgagc cgcgtctcgg cctgcgtgaa ggcgtaccag tcgcggttgt
     2281 tatcatggcc gacgtacttg tggtacagct cgggcggcga agtcccttcg tgcggcgtgc
     2341 cgagcacgcg gtcgtgccag cgcttcacga tgtccacgcc gtccgggttc agagagggaa
     2401 cgaggaggat gatcgtgttc ttcagcacgt cgcgaatctc cggctcgtcc gacgcggcga
     2461 ggcggtgcgc gatgagcgtg gaggagagcg tcgagccgac ctcggtcgag tggatgccgc
     2521 aggtgatgag gacgaccgtt ttgccgcgcg cgatgagcgc ggcgaccttg cggtctgctt
     2581 gcggcccgag cgtgcgcggg tctgcgagct ggcgctgaat ctccctgaac tcgtcgagcc
     2641 gcgcgaggtt ctcgggcgcg ctgatggtcg ccacgacgaa gggcgcgccc atcgtcgtct
     2701 tcccaagttc ttccaccttc acgcggtcgc tcgccgcgtc gagcttccga aagtaatcga
     2761 cgatggcaga ccaatcggcg agcttgcggt cgtcgcccgg ctcgaagccg agcacgtcct
     2821 tcggcgcggg cactgactgt gcaaggcgcg cgtgtgcgcc cggcgcgagg gcgagcagtg
     2881 gcgacagcag ggccgcggcg aggagtgcga agggcagaag gcgtctcggc atctgtaaga
     2941 cctcggcgtc gatgtttgcg gacgatagag cttgattcgg attggccgga gagtttagca
     3001 caccgctgcg gacagtaggc agtaggcggt aggcagtatg cagtaagaag aagggccgtc
     3061 aagacccg
//
LOCUS       DBNGOH010000307         4865 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1879974, whole genome shotgun sequence.
ACCESSION   DBNGOH010000307 DBNGOH010000000
VERSION     DBNGOH010000307.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4865)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4865)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4865
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1879974"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2685
                     /locus_tag="AC159P_05905"
     CDS             <1..2685
                     /locus_tag="AC159P_05905"
                     /inference="COORDINATES: protein motif:HMM:NF024583.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HIR3695023.1"
                     /translation="SPVVKVLRDGAVVGLANHTVLISKAGAETPIDDSGAPIKDEAGR
                     IIGVVLVFHDITERRRVEGALHESEGRYRLLFESNPQPMWVYDLDTLAFLAVNDAAIR
                     YYGYTREEFLSMTIKEIRPPEDVPRLLETVSRVTDGLDEVTGWRHLKRDGTLIDVEVA
                     SHALVFAGRRAELVLAHDITERKRAEAEMRASEARYRALADAMPQIVWTATAEGYTDY
                     YNKRWFDYTGMTLEQTRGWGWQPVIHPDDLERSLRRWATSVETGEEYRIEYRFRRAAD
                     GQYRWHLGRAEPLRDAAGRVVKWFGICMDIHDQKQTEERVRFLAEANALLASSLDYET
                     TLARLARLSVMTLADHCLIDVIGDDGRVRRVATVHASPGKEEIVEDLRRFPPTPGETG
                     GIPTVLSTGRPLVVPKLTDEKIKTLARGDEHLAALRRLGPTSFMVVPLNARGRTIGAI
                     TLGLDEPGRGYTATDLSFAEELARRAALAIDNARLYSRAQEANRTKDEFLATLSHELR
                     TPLTPVIGWVHMMRSGHLDAAGAAQGLEVIDKNAQALLRLINDLLDMTSIMSGKMRIE
                     RAPVELGRVLGEAVETVRPQADAKGIALELADGDGADGAATALVSGDRTRLVQVFWNL
                     LSNAVKFSRKGTRVRVESRTQGAAARVEVVDEGSGIETEFLPRVFDRFSQADGSTTRA
                     HGGLGLGLALVKSFIEAHGGTAEVFSDGPGRGSRFAVTLPLLRNADCGMRIEEATKVD
                     DGGAPLIEDSTPPVNQESAIRIPHSAIPRVLLVEDARDTLEMLRVVIRLRGYEAVGCA
                     SAEEALGVAPAARFDIIVSDIGLPRIDGYELIRRLRDLPHLRDTPALALTGYAAAKDA
                     EAALAAGFDAHLPKPVEPSALAEEIERLLRRSRDGDGD"
     gene            2905..3405
                     /locus_tag="AC159P_05910"
     CDS             2905..3405
                     /locus_tag="AC159P_05910"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3695024.1"
                     /translation="MPFATNQPVASRPLLASALPSPRGDRARGDADARPRAGHDAGEA
                     KAKPLALVVDDAPDVTEMLGMLMRFAGYEVVTAFGAAEAFDAARRSHFDVIISDIGMP
                     GMNGYELAEALRRLPSYAAVPLIAVTGFSMYDDRERALGSGFDDFLTKPINPSDLIDT
                     VNRLRE"
     gene            3459..4733
                     /locus_tag="AC159P_05915"
     CDS             3459..4733
                     /locus_tag="AC159P_05915"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HIR3695025.1"
                     /translation="MEGRPDKKKSARPPGRDTGGTAQGRALAAARAEMGAEGRDADPH
                     EQQAPAREQEAEAEKMRSLGQLAAGVAHDFNNALAAIVGRTQLLLRLVADDKLRRHLR
                     IIETAALDAAETVSRIQNFARRTPKGPLKTVAASALVADTVQLTRTRWETDARAQGIS
                     YEIDLRPCEDGTDQINVNPSEVREVLVNLVLNALDAMPDGGRLGLGVAGGEEAVVLRV
                     EDNGDGIASDALEHIFEPFFTTKGTGGSGLGLAVSYGIVRRHGGQIEVESELGRGTTF
                     RLRFPRARGLEISRTDLRTDLPRLRVLVVDDEDAVREVLVEMLEELKQDVTEARTAAE
                     ARDHLARGDYDLMITDLSMPDTDGLNLASEARRLTPSTRVVLATGYGQSPPAGARPDH
                     ALVDHILAKPFQLSDLEAVLWTLREGRKESEQ"
BASE COUNT          793 a         1821 c         1606 g          645 t
ORIGIN      
        1 agccccgtcg tgaaggtgct gcgcgacggc gccgtcgtcg ggctggcgaa ccacacggtg
       61 ctcatctcga aggcgggcgc cgagacgccg atcgacgaca gcggcgcccc catcaaagac
      121 gaggcgggcc ggatcatcgg cgtcgtgctc gtcttccacg acatcaccga gcggcggcgg
      181 gtcgagggcg cgctgcacga atccgagggg cgctaccgcc tcctcttcga gtcgaacccg
      241 cagccgatgt gggtctacga cctcgacacg ctcgccttcc tcgccgtcaa cgacgccgcc
      301 atccgctact acggctacac gcgcgaagaa ttcctctcca tgacgatcaa ggagattcgc
      361 ccgcccgagg acgtgccccg cctgctcgaa accgtctcgc gagtgaccga cggactcgac
      421 gaggtcaccg gctggcgcca cctcaaacgg gacggcaccc tgatcgatgt cgaggtcgct
      481 tcgcacgccc tcgtcttcgc gggccgccgc gccgagctgg tgctcgccca cgacatcacc
      541 gagcgcaagc gcgccgaggc ggaaatgcgc gcctccgagg cccgctaccg cgcgctcgcc
      601 gacgcgatgc cgcagatcgt ctggacggcc accgccgaag gctacaccga ctactacaac
      661 aagcgctggt tcgactacac cgggatgacg ctggagcaga cgcgcggctg gggctggcag
      721 cccgtcatcc acccggacga tctggagcgc tccttgcgcc gctgggcgac ctcggtcgag
      781 acgggcgagg agtaccgcat cgagtaccgc ttccgccgcg ccgcggacgg ccagtaccgc
      841 tggcacctcg gtcgcgccga gccgctgcgc gacgccgcgg gccgcgtcgt caagtggttc
      901 ggcatctgca tggacatcca cgaccagaag cagaccgaag agcgcgtccg ctttctcgcc
      961 gaggccaacg cgcttctggc ctcctcgctc gactacgaga cgacgctcgc gcggctcgcg
     1021 cgcctctccg tgatgacgct cgccgaccac tgcctgatcg acgtgatcgg cgacgacggg
     1081 cgcgtgcggc gcgtggcgac cgtccacgcc agccccggca aggaagagat cgtcgaagac
     1141 ctgcgccgct tccccccgac gcccggcgag accggcggca tccccaccgt gctcagcacg
     1201 gggcggcccc tcgtcgtccc caagttgacc gacgagaaga tcaagacgct cgcgcgcggc
     1261 gacgaacacc tcgcggcgct gcgccgcctc ggcccgacct ccttcatggt cgtcccgctc
     1321 aacgcgcgcg ggcgcaccat cggcgccatc acgctcggcc tcgacgagcc gggccgcggc
     1381 tacacggcga ccgacctctc gttcgccgaa gagctggcgc ggcgcgccgc cctcgccatc
     1441 gacaacgcgc gtctctacag ccgcgcgcag gaggccaacc gcaccaagga cgagttcctc
     1501 gccacgctct cgcacgagct gcgcacgccc ttgacgcccg tcatcggctg ggtgcacatg
     1561 atgcgctcgg ggcacctcga cgcggcgggc gcggcgcagg ggcttgaggt catcgacaag
     1621 aacgcgcagg cgctgctgcg gctcatcaac gacctgctgg acatgacctc gatcatgagc
     1681 ggcaagatgc ggatcgaacg cgcgccggtc gagctggggc gcgtcctcgg cgaggcggtc
     1741 gagaccgtcc gcccgcaggc cgacgcgaaa ggcatcgcgc tcgagctggc ggacggcgac
     1801 ggcgccgacg gggcggcgac cgcgctcgtc tccggcgacc gcacgcggct cgtgcaggtc
     1861 ttctggaacc tgctgtcgaa cgccgtcaag ttctcgcgca aggggacgcg cgtgcgcgtc
     1921 gagtcgcgca cgcagggcgc ggcggcgcgc gtcgaagtgg tggacgaagg cagcggcatc
     1981 gagacggaat tcctgccgcg cgtcttcgac cgcttcagcc aggccgacgg ctcgacgacg
     2041 cgcgcgcacg gcggcctcgg cctcggcctc gcgctcgtca agagcttcat cgaggcgcat
     2101 ggcggcaccg ccgaagtctt cagcgacggg ccgggccgcg gcagccgctt cgccgtcaca
     2161 ctccccttat tgcggaatgc ggattgcgga atgcggattg aagaggcgac gaaggttgac
     2221 gacggcggcg ctcccctgat tgaagacagc acgcctcccg tcaaccagga atccgcaatc
     2281 cgcattccgc attccgcaat cccgcgggtg cttcttgtcg aggacgcgcg cgacacgctg
     2341 gagatgctgc gggtcgtcat ccggctgcgc ggctacgagg cggtgggctg cgcgagcgcg
     2401 gaggaggctc tcggtgttgc cccggcggcg cggtttgata taatcgtctc cgacataggt
     2461 ctcccccgca ttgacggcta cgagttgata agaagactgc gagacctgcc ccacctgcgc
     2521 gacacgcccg cgctggcgct cacgggatac gccgccgcga aagacgccga ggccgccctc
     2581 gccgccggct tcgacgcgca cctgcccaag ccggtcgagc cgtcggcgct ggccgaagag
     2641 atcgaacggc tcctgcgtcg gtcacgcgac ggcgacggcg actgaagcaa gcacggcccg
     2701 cccccggcgg cgctgaaagc cgccggtgtt tagctcttta gaagtatcgt cgaaactccc
     2761 ggcacgaaga cccgtaatgc acacgcgaca gcgctccggc gctgccttcg tgttttcgcc
     2821 gggagtttca tccgccgccc ccttctttga gtcttgacga acaagggcct gctgcgtatg
     2881 cgggcgcgcc cggagtaaac gaacatgcct ttcgccacga accagcccgt cgcttcgaga
     2941 ccgctcctcg cctcggccct cccctcgccg cgcggcgacc gggcgcgcgg cgacgcggac
     3001 gcgcgccccc gcgccgggca cgacgcgggc gaggcgaagg cgaagccgct ggccctcgtg
     3061 gtggacgacg cgcccgacgt gaccgagatg ctcggcatgc tgatgcgctt cgcgggctac
     3121 gaggtggtga cggcgttcgg cgcagcagag gcgttcgacg ccgcgcgccg cagccacttc
     3181 gacgtgatca tctccgacat cgggatgccg ggcatgaacg gctacgagct ggccgaggcc
     3241 ctgcgccggc tcccgtccta cgccgccgtg ccgctcatcg ccgtcaccgg cttctcgatg
     3301 tacgacgacc gcgagcgcgc cctcggctcc ggcttcgacg acttcctgac caaacccatc
     3361 aacccgtccg acctgatcga caccgtcaac cgcctgcgcg agtgaggcgg gccgccgctc
     3421 gcccccccgc aagggccgga agcgaaagcc ctcacgtcat ggaaggcaga cccgacaaaa
     3481 agaaatccgc ccgcccgccc ggacgcgaca ccggcgggac ggcgcagggg cgcgcactcg
     3541 ccgcggcgcg cgccgagatg ggcgccgagg gccgcgacgc ggacccccac gaacagcagg
     3601 cgcccgcgcg cgagcaggag gccgaggccg agaagatgcg cagcctcggg cagctcgccg
     3661 cgggcgtcgc gcacgacttc aacaacgcgc tcgccgccat cgtcggtcgc acgcagctcc
     3721 tcctgcgcct cgtcgcggac gacaagctgc gccgccacct gcgcatcatc gagaccgccg
     3781 cgctcgacgc cgccgagacc gtcagccgca tccagaactt cgcccgccgc acgcccaaag
     3841 ggccgctcaa aaccgtcgcc gcctcggcgc tcgtggccga caccgtccag ctcacgcgca
     3901 cgcgctggga gacggacgcg cgcgcgcagg gcatctccta cgagatcgac ctccggccct
     3961 gcgaagacgg caccgaccag atcaacgtca acccctcgga ggtgcgcgag gtgctggtca
     4021 acctcgtcct caacgcgctc gacgcgatgc ccgacggcgg gcgcctcggc ctcggcgtgg
     4081 cgggcggcga ggaggcggtc gtcctgcgcg tcgaggacaa cggcgacggc atcgcgtcgg
     4141 acgcgctcga acacatcttc gagccgttct tcacgaccaa gggcacgggc ggctccggcc
     4201 tcggcctcgc cgtctcctac ggcatcgtcc gccgccacgg cggccagatc gaggtcgaga
     4261 gcgagctggg ccgcggcacc accttccgcc tgcgcttccc gcgcgcgcgc ggcctggaaa
     4321 tctcccgcac agacctgcgc acggacctcc cgcgcctgcg cgtcctcgtc gtcgatgacg
     4381 aagacgcggt gcgcgaagtc ctcgtcgaga tgctcgaaga gctgaagcag gacgtgaccg
     4441 aggcgcgcac cgccgcggag gcccgcgacc acctcgcgcg cggcgactac gacctcatga
     4501 tcacggatct ttccatgccc gacacggacg gcctcaacct cgcgtccgag gcccgccgtc
     4561 tcacgccctc gacgcgcgtc gtcctcgcca ccggctacgg ccaatcgccc cccgccggcg
     4621 cgcgcccaga ccacgccctc gtcgatcaca tcctcgccaa gcccttccaa ctctccgacc
     4681 tcgaagccgt cctctggact ttgcgcgaag ggcggaaaga gagtgaacag taggcagcag
     4741 gcagcgggca gcaggcagca ggcagcaggc agttacagcg gtggtcattc ggatgcgacc
     4801 tgatgctggc agtttgccgc cgtgttcgct agaaacgagc gtaagatggg gcatttggcg
     4861 gaagt
//
LOCUS       DBNGOH010000308         3890 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_413522, whole genome shotgun sequence.
ACCESSION   DBNGOH010000308 DBNGOH010000000
VERSION     DBNGOH010000308.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3890)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3890)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3890
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_413522"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1053
                     /locus_tag="AC159P_05920"
     CDS             <1..1053
                     /locus_tag="AC159P_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002623489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HIR3695026.1"
                     /translation="STSGWSGRGGQTLNPYALDRNPCGSSSGSGAAVAANLCAAAVGT
                     ETDGSIVCPASACGIVGIKPTLGLLGGAGIIPIAHSQDTAGPMARTVADAALLLGALA
                     ASRTDYTRALDPAGLEGARIGVARKFFGFNDKVDALMKDALDVLKRSGALLIDPADIP
                     THGQFDASEFEVLLYEFKADLNKYLASLAPGDHPRTLKALIDFNERHKEKEMPYFGQE
                     IFIKAEAKGPLTERAYRLALAKNLRLSRAQGIDAVMRKHRLDAIVAPTGGPAWTTDLV
                     NGDHFTGGSSTPAAVSGYPNINVPAGFVAGLPVGLSFFGRARSEPTLIRLAYAYEQAT
                     KHRRPPRFLPRADLRP"
     gene            1157..2806
                     /gene="boxC"
                     /locus_tag="AC159P_05925"
     CDS             1157..2806
                     /gene="boxC"
                     /locus_tag="AC159P_05925"
                     /EC_number="4.1.2.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008162794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_process: GO:0010128 - benzoate catabolic process via
                     CoA ligation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-epoxybenzoyl-CoA dihydrolase"
                     /protein_id="HIR3695027.1"
                     /translation="MTDSGRMINFETTPEAYKHWRLSVEGAVATLSMDVQEDETLREG
                     YKLKLNSYDLGVDIELADAVQRLRFEHPEVRAVVVTSLKPRIFCAGANIYMLGTSSHA
                     FKVNFCKFTNETRCSIEDASRNSGQRYVAALNGTASGGGYELAIACDEIYLVDDGNSA
                     VSLPEVPLLGVLPGTGGLTRLVDKRKVRRDRADVFSTLAEGLKAKRAQEWGLIDGHFP
                     TSKFQEGTAARVCEIAEAGGGQEIGNGIKLNPLEAEAGDAGREYKYVSVKYNRAGRYA
                     DLTVRGPRGEQPSKPEEIVELGDQYWPLRAYRELDDALLHLRMNEPEIGLVCVRTEGS
                     AADVLKVDETLVEHRDHWLVREIILHMGRVLRRLDLTAKSFFALAEPGGCFAGNLLEL
                     LLAADRVYMLNDPEQKIELAAGELNAGALPMSNGLTRLGSRFLAAPERADAVLAHEGA
                     FDADEAEEFGLVTFAPDELDWEDEVRVAIEERTSLSPDALTGMEASLRFAGPETMDTK
                     IYGRLTAWQNWIFQRPNAVGPQGALTNYGKPTQPQFDYKRT"
     gene            2927..>3890
                     /gene="boxB"
                     /locus_tag="AC159P_05930"
     CDS             2927..>3890
                     /gene="boxB"
                     /locus_tag="AC159P_05930"
                     /EC_number="1.14.13.208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006975920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0010128 - benzoate catabolic process via
                     CoA ligation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoyl-CoA 2,3-epoxidase subunit BoxB"
                     /protein_id="HIR3695028.1"
                     /translation="MELYERIPNNVNLSEDKRLQRALEKWLPDYLDWWREMGPEGFQE
                     KDVWLRTAISVDTDGWAHFDYVKMPDYRWGIFLKPADPGATVGFGDNFGRPAFQEVPG
                     EFRNALRRIIVTQGDTEPASVEQQRRLGHTCPSLYDMRNLFQVNVEEGRHLWAMVYLL
                     HKYFGRDGRDEADAMLERRSGHPDKPRILEAFNQPCDDWLNFFCFTMFTDRDGKYQLS
                     ALAESGFEPLARTCQFMLTEEAHHLFVGETGVGRVVKRTAELMKLNPNEDVRHLGGIP
                     LDMIQRHINFWFSYSLELFGGEISSNSADFFAAGLKGRYQEQKKY"
BASE COUNT          701 a         1331 c         1245 g          613 t
ORIGIN      
        1 tcgacgagcg gttggagcgg gcgcggcggc cagaccttga acccttacgc gctcgaccgc
       61 aacccctgcg gctcaagctc cggctcgggc gcggcggtcg ccgccaacct ctgcgcggcg
      121 gcggtcggca ccgaaacgga cggctccatc gtctgccccg cttcggcctg cggcatcgtc
      181 ggcatcaagc ccacgctcgg cctcctcggc ggcgccggca tcatccccat cgcgcacagc
      241 caggacaccg ccggcccgat ggcgcgcacc gtcgccgacg cggcgctgct tctcggcgcg
      301 ctcgccgctt cgcggacgga ctacacgcgc gcgctcgacc ccgccggact cgaaggcgca
      361 cgcatcggcg tcgcgcgaaa attcttcggc ttcaacgaca aggtggatgc gttgatgaag
      421 gacgcgctcg acgtgttgaa aagaagcggc gctcttctca tcgacccggc ggacatcccc
      481 acgcacggcc agttcgacgc ctccgagttc gaggtgctgc tctacgagtt caaggccgac
      541 ctcaacaagt acctcgcctc gctcgcgccc ggcgaccacc cgcgcacgct caaggcgctg
      601 atcgacttca acgagcgcca caaagaaaag gagatgccct acttcggcca ggagattttc
      661 atcaaggccg aggcgaaggg gccgctcacc gagcgcgcct accgcctcgc gctcgccaaa
      721 aacctgcgcc tctcgcgcgc gcaagggatc gacgccgtga tgcggaagca ccgcctcgac
      781 gccatcgtcg cgcccaccgg cggccccgcg tggacgaccg acctcgtcaa cggcgaccac
      841 ttcaccggcg gcagctccac gcccgccgcc gtctccggct accccaacat caacgtcccc
      901 gccggcttcg tcgcgggcct gcccgtcggc ctctccttct tcggtcgcgc ccgctccgaa
      961 cccaccctca tccggctcgc ctacgcctac gagcaggcca ccaaacaccg ccgcccaccg
     1021 cgcttcctgc cgcgggcaga cttgcggcct tgaggagttt cgccgccgcg gcgaaatgca
     1081 ttcctgcctt agcgaaggta taattgttgt ttcgtgccgc ggcacttatt ttggctgttg
     1141 gagtctgtga ggacggatga ctgattcagg acggatgatt aatttcgaga cgacgcccga
     1201 agcgtacaag cactggcggc tgtcggttga aggggccgtg gccacgctct cgatggacgt
     1261 gcaggaggac gagacgctgc gcgagggcta caagctgaag ctcaactctt acgacctcgg
     1321 cgtggacatc gagctggccg acgccgtgca gcgtctgcgg ttcgagcacc ccgaggtgcg
     1381 cgcggtcgtc gtcaccagcc tgaagccgcg catcttctgc gcgggcgcga acatctacat
     1441 gctcggcacc tcttcgcacg ccttcaaggt caacttctgc aagttcacga acgagacgcg
     1501 ctgctccatc gaggacgcct cgcgcaactc cggccagcgc tacgtcgcgg cgctcaacgg
     1561 cacggcctcg ggcggcggct acgagctggc catcgcctgc gacgagattt atctggtgga
     1621 cgacggcaac tcggcggtct cgcttcccga agttcctttg ctcggcgtgc tgccgggcac
     1681 aggaggtctc acgcgcctcg tcgataagcg caaggtgcgg cgcgaccgcg ccgatgtgtt
     1741 ctcgacgctg gccgaggggc tgaaggcgaa gcgcgcgcag gagtggggcc tcatcgacgg
     1801 ccacttcccg accagcaaat ttcaggaagg cacggcggcg cgcgtgtgcg agatcgccga
     1861 agcgggcggc gggcaggaaa tcggcaacgg catcaagctg aacccgctcg aagcggaggc
     1921 gggcgacgcg gggcgcgagt acaagtacgt ctccgtgaaa tacaaccgcg cgggccgcta
     1981 cgccgatttg acggtgcgcg gcccccgcgg cgaacagcct tcgaagccgg aggagatcgt
     2041 cgaactcgga gaccagtact ggcccctgcg cgcctatcgc gagctggacg acgcgctgct
     2101 ccatctgcgg atgaacgagc cggagatcgg ccttgtctgc gtcaggacgg aaggcagcgc
     2161 cgcggacgtt ctgaaggtag acgagacgct ggtcgagcac cgcgaccact ggctcgtgcg
     2221 cgagatcatc ctgcacatgg gccgcgtcct gcgccgcctc gatctgacgg ccaagagctt
     2281 cttcgcgctc gccgagccgg gcggctgctt cgccggcaac ctgctcgaac tgctgctcgc
     2341 ggcagaccgc gtctacatgc tgaacgatcc cgagcagaag atagagctgg ccgcgggcga
     2401 gttgaacgcg ggcgcgctgc cgatgtcgaa cgggctgacg cgcctcgggt cgcgcttcct
     2461 cgccgcgccg gagcgggccg acgcggtgct cgcgcacgaa ggcgccttcg acgccgacga
     2521 ggccgaagag ttcggactcg tcaccttcgc gcccgacgag ctggactggg aagacgaggt
     2581 gcgcgtcgcc atcgaggagc gcacgtcgct ctcgcccgac gcgctcacgg ggatggaggc
     2641 gagtctccgt ttcgccggcc ccgaaaccat ggacacgaaa atctacgggc ggctgacggc
     2701 gtggcagaac tggattttcc agcgccccaa cgccgtcggc ccgcagggcg cgttgaccaa
     2761 ctacggcaag cccacgcagc cgcagttcga ttacaagcga acatgaaagc cgtgaaccgt
     2821 gataagtgaa aagcgatgag atgtaaccag tgacgagtag caagctgaat cctgctttta
     2881 ctcatcacgg ttcacggttc acggttcacg gctttcgagg aagaacatgg aattatacga
     2941 gcgcatcccg aacaacgtga atctgtcgga ggacaagcgg ctgcaacgcg cgcttgagaa
     3001 gtggctgccc gattatcttg attggtggcg cgagatgggg ccggagggct ttcaggagaa
     3061 ggacgtgtgg ctgcgcaccg ccatcagcgt ggacacggac gggtgggcgc acttcgacta
     3121 cgtgaagatg ccggactacc gctggggcat cttcctcaag cccgcagacc ccggcgccac
     3181 cgtcggcttc ggcgacaact tcggcaggcc cgccttccag gaagtgcccg gcgagttccg
     3241 caacgccttg cgccgcatca tcgtcacgca gggcgacacc gagccggcct cggtcgagca
     3301 gcagcgccgt ctgggccaca cctgcccctc gctctacgac atgcggaacc tctttcaggt
     3361 gaacgtcgaa gagggccgcc acctgtgggc gatggtctac ctgctccaca agtatttcgg
     3421 gcgcgacggg cgcgacgagg ccgacgcgat gctggagcgc cgctcgggcc atccagacaa
     3481 gccgcgcatc ctcgaagcct tcaaccagcc gtgcgacgac tggctcaact ttttctgctt
     3541 cacgatgttc accgaccgcg acggcaagta ccagctttcg gcgctcgccg aatcgggctt
     3601 cgagccgctg gcgcgcacct gccagttcat gctgacggag gaggcacacc acctcttcgt
     3661 cggcgagacg ggcgtcggtc gcgtcgtcaa gcgcacggcg gagctgatga agctgaaccc
     3721 gaacgaggac gtgcggcact tgggcggcat cccgctcgac atgatccagc gccacatcaa
     3781 cttctggttc tcctactcgc tcgaactgtt cggcggcgag atcagctcga actccgcgga
     3841 cttcttcgcc gcgggcctca aagggcgcta tcaggagcag aagaagtacg
//
LOCUS       DBNGOH010000309         5422 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1330642, whole genome shotgun sequence.
ACCESSION   DBNGOH010000309 DBNGOH010000000
VERSION     DBNGOH010000309.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5422)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5422)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5422
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1330642"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1152
                     /locus_tag="AC159P_05935"
     CDS             <1..1152
                     /locus_tag="AC159P_05935"
                     /inference="COORDINATES: protein motif:HMM:NF014342.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HIR3695029.1"
                     /translation="VGVFVSETAHADVADTYSPAQGLIVHKVTVAKGGLKLGDKVAAI
                     VDAEKRDATRRNHTATHLMHAALREVLGTHVKQAGSVVAPNYLRFDFAHYQPLTRDEI
                     AEIERLVNFQILRNERVRTDVLTLEEAMRSGAMALFGEKYAERVRVLSIPGVVEGEAF
                     SKELCGGTHVRATGDIGLFKITSDESIASGTRRIRAVTGQDAFLRFQETEALVDQLSA
                     ELRATRPDIPAAIGRLQEELKKSRRESDELRMKLAMGGGSASNGGEPREVAEGVRVLA
                     REASDLDAQALRQLSDTLLAQIKSGVVVLGRRADGKASLIVRTSSDLSKRVPAGQVIK
                     ELAPIIGGRGGGKPDMAEGGGPEPQKLAEALEASYAVVESLVTKTGAGA"
     gene            complement(1329..1685)
                     /locus_tag="AC159P_05940"
     CDS             complement(1329..1685)
                     /locus_tag="AC159P_05940"
                     /inference="COORDINATES: protein motif:HMM:NF013625.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HIR3695030.1"
                     /translation="MKKLLAGAFAALLSLSVAAAAQSTNPPAASQNDNANASAAKKRG
                     PVFRATKDQIKQAQTLLKGRGLYAGEATGKLDTDTRAALRKYQEAEGIKVTGTLNRVT
                     LEKMSIVLTDRQKTIS"
     gene            2053..2886
                     /locus_tag="AC159P_05945"
     CDS             2053..2886
                     /locus_tag="AC159P_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005223883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008933 - peptidoglycan lytic
                     transglycosylase activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HIR3695031.1"
                     /translation="MNKSLLLFFCALLLSAASAHAQTGVRYHFDNFDTRDGVRVETPA
                     AAAPAATKPRRMKLTARSFAPAKTASSDIAGPTYQPTAAVPMAVAKSLDGFSTGDASV
                     DALIVESGTRHGVDPVLLYAIMHRESAFKKRAVSHAGARGLMQLMPGTAARFGVRDIF
                     DPRQNIEAGTRYMRFLLNMFDGDVGLALAGYNAGEGAVLKYGRRVPPYAETREYVRRI
                     TERYALMRDPQTARRAPVATRAQVAAIKSAEPAAAPATIYERNVFAVRMPDGKLQLVS
                     Q"
     gene            3115..4569
                     /locus_tag="AC159P_05950"
     CDS             3115..4569
                     /locus_tag="AC159P_05950"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3695032.1"
                     /translation="MSETSTTPTPNARDSVANARRALVVGEAADAEALREKLAACGYV
                     VMCVTAGDAARAASKFLPALALFAFGAREGEGRLVASARRLRSLPETFALPFVFLFRT
                     DERTLRGAALNVGADDYFDIETMSAEMCARLEALFWRAEVGRRAAPVVADQLTEIDKV
                     LFLLDAVGADASDGGVPGTIALVGLAGESARAGEGEQERSLAAAHGFLKLNLRRVDAV
                     AFYGPTMLLVYLPRTDARTAQTALSRLRAEFVATQPGADLAAGLASFPSEEDDKVEAL
                     VEKAESALAQARSDETRARVAAYQSREELTPSARRAPASEALQEEEGAAAKEHRRTGG
                     TSEPPPRGAADAPGVREGERDAPVAGAAGRLLLAVSDAARMAQVNLLMRSAGYEVRAA
                     FDGRQALNLLRIERPDLVLVDSALDDMDGLEMLKRFRKQSGGKTRPSVVLMLPAGREG
                     ARAEALEAGARAVVTLPYDPVELLDVVRANGAAE"
     gene            4579..5301
                     /locus_tag="AC159P_05955"
     CDS             4579..5301
                     /locus_tag="AC159P_05955"
                     /inference="COORDINATES: protein motif:HMM:NF015648.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HIR3695033.1"
                     /translation="MNHCSNCGERLAGAARFCAACGAPADSPHDSEVTRYAAPREDAQ
                     RPASEETLVAPPRTPHTTLAPQPVRPLAAADAPGSRRVEERDDIEPVIFRVRPTLLFI
                     KLGYALAALGGLLLIALLTFLGTRWGFFNVPFYVSIPLALALLLVPAYRHVKRNMLRY
                     TLTDSKIEIDEGFISRKTRNIPLRNIQDVSVTTTIPQRLLRFGNVVVDNASDSAGATV
                     LRNIPDPRRHADQILRELRRWN"
BASE COUNT          858 a         1885 c         1839 g          840 t
ORIGIN      
        1 gtgggcgtct tcgtgagcga gacggctcac gccgatgtcg ccgacactta ctcgcccgcg
       61 caggggctga tcgtccacaa ggtcacggtc gcgaagggcg gcttgaagct cggcgacaag
      121 gtggcggcga tagtggacgc ggagaagcgc gacgcgacgc ggcgcaacca cacggcgacc
      181 cacctgatgc acgcggcgct gcgcgaagtt ttggggacgc acgtcaagca ggccggctcg
      241 gtcgtcgcgc cgaactattt gcgcttcgac ttcgcgcact accagcctct gacgcgcgac
      301 gagatcgcgg agatcgagcg gctcgtcaat ttccaaatcc tgcgcaacga gcgggtgcgg
      361 accgacgtgc tcacgctcga agaggcgatg cgctcgggcg cgatggcgct cttcggcgag
      421 aagtacgccg agcgcgtgcg cgtcctcagt attccgggcg tggtcgaggg cgaggcgttc
      481 tcgaaggagc tgtgcggcgg cacgcacgtc cgcgcgaccg gcgacatcgg cctcttcaag
      541 atcacgagcg acgagtccat cgcctccggc acgcgccgca tccgcgccgt caccggacag
      601 gacgcgttcc tgcggttcca ggagaccgaa gcgctcgtcg atcagctctc ggcggagctg
      661 cgcgcgacgc ggcccgacat ccccgcggcc atcggcagac ttcaggaaga gctgaagaag
      721 tcgcggcgcg agtcggacga actgcggatg aagctggcga tgggcggcgg ctcggcgtcg
      781 aacggcggcg agccgcgcga agtcgccgag ggcgtccgcg tgctcgcgcg cgaggcgagc
      841 gacctcgacg cgcaggccct caggcagctc tccgacacgc tgctcgcgca gatcaagtcg
      901 ggcgtcgtcg tgctgggccg gcgcgccgac ggcaaggctt cgctcatcgt ccgcacctct
      961 tcagacctct cgaagcgtgt gcccgcaggg caggtcatca aggaactcgc gccgatcatc
     1021 ggcgggcgcg gcggcggcaa gcccgacatg gccgagggcg gcggccccga gccgcagaaa
     1081 ctcgcggagg cgctcgaagc gagttacgcc gtcgtcgaga gcctcgtcac gaagaccggc
     1141 gccggcgcct aagaggaagg ggaaaaggtt aaaaggggaa agggcgaaaa gggagacaag
     1201 aagcagcgtg cctctcatca acgatgatga gaggcacgct gtttttagtc ctcattcgag
     1261 gatgagaagg agcgtgcttc tcatcctccc ttttcgccct ttcccctttt aacctcttcc
     1321 ccctttgctc atgagatggt tttctgtctg tccgtcagca cgatgctcat cttctcaagg
     1381 gtgacgcggt tgagcgtccc agtcactttg atcccctcgg cctcctgata cttcctgagt
     1441 gcggcgcggg tgtccgtgtc gagcttgccg gtggcctcgc ccgcgtacag cccacggcct
     1501 ttgaggagag tctgcgcctg cttgatctga tccttggtgg cgcggaagac ggggccgcgt
     1561 ttcttcgcgg cgctcgcgtt cgcgttgtcg ttctgtgagg ccgcaggggg gttcgtgctc
     1621 tgcgcggcgg cggcgacaga gagggacagc agcgccgcga aggcgccggc caacagcttc
     1681 ttcatcatcg tctccttaat tgtgtgaggc gcgccgcagg gcggcgaggc gcgcgggttg
     1741 aggttggtta ttcccgctcg ttcgaaagag gctccaatca tacacgcctt gtcaagaccc
     1801 cgccgaggca aactcgcgaa agcccccacg accccgcacc agcaccttca cgcgcccctt
     1861 aaatttagta atagtttagg ttaaacacat tagtaaatcc tgttgaacac gggccgaggg
     1921 ttgtgttatg ctctcttttg cgagcgttga ttaagccgac ggctcgttct cctctcaagc
     1981 cgtcgcccga agtgccacgc caataattct ccctggacag ccccggcgcc cgtgccgccc
     2041 cgaagtgttg ttatgaacaa gtccctcctg cttttcttct gcgcgctcct cctctccgcg
     2101 gcctccgccc acgcgcagac cggcgttcgc taccacttcg acaacttcga cacgcgcgac
     2161 ggcgtgcgcg tcgagactcc cgccgcggcc gcgcccgccg cgacgaaacc gcggcgcatg
     2221 aagctgacgg cgcgcagttt cgcgcccgcg aagaccgcgt cgtccgatat cgcggggccg
     2281 acctaccagc cgacagccgc cgtgccgatg gcggtcgcca agtcgctcga cggcttctcg
     2341 accggcgacg cgagcgtgga cgcgctcatc gtcgagtcgg gcacgcgcca cggcgtcgat
     2401 cccgtcctgc tctacgcgat catgcaccgc gaatcggcct tcaagaagcg cgccgtctcg
     2461 cacgcgggcg cgcgcgggct gatgcagctc atgccgggca ccgccgcgcg cttcggcgtg
     2521 cgcgacatct tcgacccgcg ccagaacatc gaggccggca cgcgctacat gcgtttcctc
     2581 ctcaacatgt tcgacggcga cgtggggctg gcgctcgcgg gctacaacgc gggcgagggc
     2641 gcggtgctga agtacgggcg gcgcgtgccg ccttacgccg agacgcgcga gtacgtgcgg
     2701 cgcatcaccg agcgctacgc gctgatgcgc gacccgcaga ccgcgcgccg cgcgcccgtc
     2761 gccacgcgcg cgcaggtcgc cgccatcaag tccgccgagc cggcggcggc gcccgcaacc
     2821 atctacgagc gcaacgtgtt cgccgtacgg atgcccgacg gcaaactcca gcttgtcagt
     2881 cagtgaaggg ccggggccga agacgcggaa cccgaagggg cgagaggccg aggtttcgaa
     2941 tcgaacgagt gagacggggc gggtttacaa tacctgcccc gttttctttt ttacggcgcg
     3001 gcctgtcgcg gtatgattgg cgcgcgacgc aaggtacgaa ccgatgcgtg gccgccccgc
     3061 gcgaggtgcg acgccaggaa cacgcgcggc ggcccggacg taaagacgcc taccatgtcg
     3121 gagacctcga caacaccaac gccgaacgcg cgcgactcgg tggccaacgc gcgccgcgcc
     3181 ctcgtcgtgg gcgaggccgc ggacgccgag gcgctgcggg agaaactcgc ggcgtgcggc
     3241 tacgtggtga tgtgcgtgac ggcgggcgac gcggcgcggg ccgcctcgaa gtttttaccg
     3301 gcgctcgcgc tcttcgcttt cggcgcgcgc gaaggcgaag gccgactggt cgcgtcggcg
     3361 cggcgcctgc gctcgctgcc ggagacgttc gcgctgccct tcgtcttcct cttccgcacg
     3421 gacgagcgca cgctgcgcgg cgcggcgctc aacgtcggcg ccgacgacta ctttgacatc
     3481 gagacgatga gcgccgagat gtgtgcgcgc ctcgaagcgc tcttctggcg cgccgaggtg
     3541 ggccgccgcg ccgcgccggt cgtcgcagac caactcacgg agatcgacaa ggtactcttc
     3601 ctcctcgacg cggtcggcgc tgacgcgtcc gacggaggcg tgccgggcac gatagctctc
     3661 gtcggcctcg cgggcgagtc cgcgcgcgcg ggcgaaggcg agcaggagcg ctcgctcgcg
     3721 gcggcgcacg gcttcctgaa gctcaacctg cgccgcgtcg atgcggtcgc cttctacggc
     3781 ccgacgatgc tgctcgtcta cctgccgcgc accgacgcgc gtacggcgca gaccgcgctc
     3841 tcgcgcctgc gcgcggagtt cgtcgcgacg caaccgggcg ccgacctcgc agccggcctc
     3901 gcctccttcc cttcggaaga agacgacaag gtcgaagccc tcgtcgagaa ggcggagagc
     3961 gccctcgcgc aggcgcgttc ggacgagacg cgggcgcgcg tcgccgccta ccagtcgcgc
     4021 gaggagttga cgccgagcgc ccgccgagcg cccgcgtccg aagcgttgca ggaagaggag
     4081 ggcgcggcgg cgaaagagca ccgccggacg ggtggaactt cggagcctcc gccgcgcggc
     4141 gccgccgacg cgccgggtgt ccgcgaagga gaacgggacg cgcccgtggc gggggcggcg
     4201 ggacgcctcc tgctcgcggt ctcggacgcg gcgcggatgg cgcaggtcaa cctgttgatg
     4261 cgttcggcgg ggtacgaggt gcgcgccgcg ttcgacggcc ggcaggcgct caacctcctg
     4321 cgcatcgagc ggcccgacct cgtgctcgtg gactccgcgc tcgacgacat ggacgggctt
     4381 gagatgttga agcgtttccg caaacagtcg ggcgggaaga cgaggccgtc ggtcgtgctg
     4441 atgctgcccg cggggcgtga aggtgcgcgc gcggaggcgc tggaagccgg cgcgcgggcc
     4501 gtcgtcacct tgccttacga tcccgtcgag ttgctcgatg tcgtgcgcgc gaacggcgcc
     4561 gccgagtgag gaggccgaat gaaccactgc tcgaactgcg gtgagcgtct cgccggtgcg
     4621 gcgcgcttct gcgccgcgtg cggcgcgccc gccgactcgc cacacgattc ggaagtgacg
     4681 aggtacgccg cgccgcgcga agacgcacaa aggcccgcgt ccgaagagac gctcgtcgcg
     4741 ccgccgcgca cgccgcacac gacactcgcg ccgcagcccg tgcgcccgct cgcggcggcg
     4801 gacgcgcccg gctcgcgccg cgtcgaggag cgcgacgaca tcgagccggt catcttccgc
     4861 gtgcgcccga cgctgctctt catcaagctc ggctacgcgc tcgccgcgct cggcgggctg
     4921 ctgctcatcg cgctgctcac gttcctcggc acgaggtggg gcttcttcaa cgtcccgttc
     4981 tacgtctcga tcccgctggc gctggcgctg ctgctcgtcc ccgcttaccg ccacgtcaag
     5041 cgcaacatgt tgcgctacac gctcaccgac tcgaagatcg agatcgacga gggcttcatc
     5101 tcgcgcaaga cgcgcaacat ccccctgcgc aacatacagg acgtgtccgt caccacgacc
     5161 atcccgcagc gcctgctccg cttcggcaac gtcgtcgtcg acaacgcgtc cgacagcgcc
     5221 ggcgcgaccg tgctgcgcaa catccccgac ccgcgccgcc acgccgacca aatcctgcgc
     5281 gagctgcgcc gctggaactg aacgcccccg cggacttatc atcgaacgcg cggaaccggc
     5341 acaacggcgc cgttgtctaa ctcatccaaa cgttttgccg taaagcgcaa aggctccgtt
     5401 cggccgtgcg ccattttgtc cg
//
LOCUS       DBNGOH010000310         4218 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3713076, whole genome shotgun sequence.
ACCESSION   DBNGOH010000310 DBNGOH010000000
VERSION     DBNGOH010000310.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4218)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4218)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4218
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3713076"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..315
                     /locus_tag="AC159P_05960"
     CDS             <1..315
                     /locus_tag="AC159P_05960"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011395479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HIR3695034.1"
                     /translation="SASLKGVGPDEEPVLLLTHNPDIFPDVPARVSLTLAGHTHGGQV
                     NFPFVGRLVVPSRYGQRYAMGHVVEGGRHLFVSGGVGTSIIPVRFRVPPEVVILTLES
                     ER"
     gene            424..1728
                     /locus_tag="AC159P_05965"
     CDS             424..1728
                     /locus_tag="AC159P_05965"
                     /inference="COORDINATES: protein motif:HMM:NF017760.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3695035.1"
                     /translation="MATAGGTARVGGGRGAGGMFSALAHRNFRLFWAGAFLSNTGTWT
                     QTVAQGWLVLQLTDSASWLGVDSFMATAPALLLTLLGGVFADLVDRKRLLILTQVGAG
                     GSALALAALLAAGVVEGPGDVWFVLALTFATGCCWAIAGPSYQAFAYDLVGREDLANA
                     VALNSAQFQLSRVVGPVLAALTIQLFGLAGCFLVNGLSYGAIVLALSRVRFADKVEGQ
                     AVDERAGAGAHSLRDGRALWRDLVEGVRYARARPRVRLLMLCTAVVSVFGSPYLTLMP
                     LFARNVFGWGETGLSLLMGTAGAGALAGALVIAYLGDFKRKGWLVLGAALSAGLCLMA
                     FAWAPYAPLALAVLFVTGGSMVCFFSVSNTLLQQLVTDRMRGRVMSIWIMCFIGAMPF
                     GNLLGGKAADMFGPRLTVLACGLSISLFVSLVGLRERRMGEI"
     gene            complement(1844..2767)
                     /locus_tag="AC159P_05970"
     CDS             complement(1844..2767)
                     /locus_tag="AC159P_05970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695036.1"
                     /translation="MKRLLLLFFATCFSLPAVSQAAAQETPPPPPNFVLIVREEIKPG
                     EMPAHEREGAAFVQTLAKANARIADPKLRSGRIALSPIAGNENEVMYLWPYDSFDDME
                     KSQAEIDKMAGDPKLLKADFDQLPDARLHVSQRDMIASYRPDLSYKVGRLDVAQARYM
                     TMQTVRIKPGHEDEYWEMMKEKMFPARDKAGIKASYAVFQVIGGAPGTTFFVFRPFRS
                     LAELDERAPVLTRNAMSEETRKEVDKVVDRAVIFQDVTFYRINPRLSHVAPEFAARDA
                     ASATPFWNPKPEAPAPAAGRRAVARSNGRRP"
     gene            complement(3090..3602)
                     /locus_tag="AC159P_05975"
     CDS             complement(3090..3602)
                     /locus_tag="AC159P_05975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695037.1"
                     /translation="MRNLSRHYLRKHWLSLCTTVLVLYTVIPVRQEDLYDLGALTARL
                     ELLMFVLSFPLGSLFMYAFYTGTGGIGERILLWMMALALGYVQWFHLVPALLARRRPA
                     VTTLNLSPDNMVASAPAAAPASAPGPPPEKQLDAAPAPPVPQFDARGLTPLERIIRGA
                     DDGRAATHDG"
     gene            complement(3660..3860)
                     /locus_tag="AC159P_05980"
     CDS             complement(3660..3860)
                     /locus_tag="AC159P_05980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695038.1"
                     /translation="MGLLKLRLLPFALAVALGAACDAYVRRPDDPGRTQCFRTSWKCY
                     VVSIADHPVDFLRASWVMLTEG"
     gene            4034..>4218
                     /locus_tag="AC159P_05985"
     CDS             4034..>4218
                     /locus_tag="AC159P_05985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695039.1"
                     /translation="MRTVRDLLCLVFGVLLLALAGAATANAQGVIVPGPCRRCPVPPR
                     PIALPRALPVKSVKIET"
BASE COUNT          614 a         1342 c         1531 g          731 t
ORIGIN      
        1 tccgcgagcc tgaagggagt cgggccggac gaagagcccg tgctcctgct cacgcacaac
       61 ccggacatct tccccgacgt gcccgcgcgc gtcagcctca cgctcgcggg acacacgcac
      121 ggcgggcagg tcaacttccc cttcgtcggc aggctcgtcg tcccttcgcg ctacggccag
      181 cgctacgcga tgggtcacgt cgtcgaaggc gggcgccacc tcttcgtcag cggcggcgtc
      241 ggcaccagca tcatccccgt ccgcttccgc gtcccgcccg aagtcgttat cctcacgctg
      301 gaaagtgagc gttgagcggt gagcggttga aacggagggc gcacgaggca ctcgactttc
      361 tacggcccgc cgcccacttc cggggcgcgg gcgttatcga ctctcggagc agtcataaag
      421 ttcatggcga cggcaggggg cacggcgcga gtcgggggag ggaggggcgc gggcgggatg
      481 ttcagcgcgc tcgcgcaccg caacttccgc ctgttctggg cgggggcttt tttgtcgaac
      541 acggggacgt ggacgcagac ggtcgcgcag gggtggctcg tgctgcaact gacggactcg
      601 gcgtcgtggc tcggcgtgga cagcttcatg gcgacggcgc cggcgctgct gctgacgctc
      661 ttgggcggcg tcttcgcaga cctcgtggat cgcaagcggc tgctcatcct gacgcaggtg
      721 ggcgcaggcg ggtcggcgct ggcgctcgcg gcgctgctcg cggcgggcgt ggtcgaaggc
      781 cccggagacg tgtggttcgt cctcgcgctg acgttcgcca ccggctgctg ctgggccatc
      841 gcggggcctt cgtaccaggc gttcgcgtac gacctcgtcg ggcgcgaaga cctcgccaac
      901 gccgtcgcgc tcaactcggc gcagttccag ttgtcgcgcg tggtcggccc cgtgctcgcc
      961 gcgctgacga tccagctctt cggcctcgcc ggctgcttcc tcgtcaacgg cctctcctac
     1021 ggggccatcg tgctggcgct ctcgcgggtg cgcttcgcgg acaaggtcga ggggcaggcg
     1081 gttgacgaga gggccggtgc cggggcgcac tcgctgcggg acgggcgtgc gctgtggcgc
     1141 gatctggtgg agggcgtgcg ctacgcgcgg gcgcgaccgc gggtgcggct gctgatgctg
     1201 tgcacggcgg tggtgagcgt gttcgggtcg ccgtacctga cgctgatgcc gctcttcgcg
     1261 cgcaacgtgt tcgggtgggg cgagacgggc ctctcgctgc tcatgggcac ggcgggtgcg
     1321 ggcgcgctcg cgggcgcgct cgtcatcgcc tacctcggcg acttcaagcg caaggggtgg
     1381 ctcgtgctcg gcgccgcgct ctcggcgggg ctgtgcctga tggcgttcgc gtgggcgccg
     1441 tacgcgccgc tcgcgctggc ggtgctgttc gtgacgggcg gctcgatggt ctgcttcttc
     1501 tcggtctcga acacgctcct gcaacagctc gtgacggacc ggatgcgcgg tcgcgtgatg
     1561 agcatctgga tcatgtgctt catcggcgcg atgccgttcg gcaacctgct cggcggcaag
     1621 gccgcggaca tgttcggccc gcgcctgacg gtgctcgcgt gcggcctctc catctcgctc
     1681 ttcgtctccc tcgtcggcct gcgcgaaagg cgcatgggcg agatttgaag aggcgatgaa
     1741 cgcggaacgc ggaatgatga attattgtca ggcatcgggc gccggttttt ccccgacgcc
     1801 cgacacccgc tgttcattca tcatcccgcg ttccgcatct ctcttacggt cgcctgccgt
     1861 tactacgcgc gaccgccctc ctgccggcgg cgggggcggg cgcttccggc ttgggattcc
     1921 agaagggggt ggccgacgcg gcgtcgcgcg ccgcgaactc cggcgccacg tggctgaggc
     1981 gcgggttgat gcggtagaag gtcacgtcct ggaagatgac ggcccgatcc acgaccttgt
     2041 ccacctcctt ccgggtctct tcgctcatcg cgttgcgcgt caagaccggc gcccgctcgt
     2101 ccagctcggc cagcgagcgg aagggccgga agacgaagaa ggtcgtgccg ggcgcgcccc
     2161 cgatgacctg aaagaccgcg taggaggctt tgatcccggc cttgtcgcgc gcggggaaca
     2221 tcttctcctt catcatctcc cagtattcgt cctcgtggcc gggcttgatg cgcaccgtct
     2281 gcatcgtcat gtagcgcgcc tgcgccacgt cgagcctgcc gaccttgtag ctcaaatcgg
     2341 gtcggtagga ggcgatcatg tcgcgctgcg agacgtggag cctggcgtcc gggagctggt
     2401 cgaagtcggc cttcaagagc ttcgggtcgc ccgccatctt gtcgatctcg gcctggctct
     2461 tctccatgtc gtcgaacgag tcgtagggcc acaggtacat cacctcgttc tcgttgccgg
     2521 caatcggcga cagggcgatg cgcccggagc gcagcttcgg gtcggcgatg cgcgcgttag
     2581 ccttcgccag cgtctggacg aaggcggcgc cctcccgctc gtgcgcgggc atctctccgg
     2641 gcttgatctc ctcgcgcacg atcagcacga agttgggcgg cggcggcggc gtctcctgcg
     2701 ccgcggcctg tgaaacagcc ggaagggaaa aacacgtggc gaaaaaaaga agaagcaatc
     2761 tcttcatggg tggcccacct ccggggggct tgtgttagaa gtggaaggaa ggtgaaaccg
     2821 tctctccggc acggacgcgg ggttgcgaac tccggagtag gtctgtcagc gggaagcgcg
     2881 gcggcccgcg gcaactcggc gagcggcgct ttcgtgccat ctaaggccgt tggcgggggc
     2941 gagggcaggt gccgccccgc gcggccaggc gtgttcgacc tttcggcggc ggacaatatt
     3001 agaaacggcg cggcaagtca atggcgaaga acacaacgag gcatggccgc acgcgaggcc
     3061 ggcccgacgc gccgaagggg gcgcgccttc tatccgtcgt gtgtcgcggc gcgcccgtca
     3121 tccgctccgc ggatgattct ttcgaggggc gtgaggccgc gggcgtcgaa ttgcgggacg
     3181 gggggcgcgg gggcggcgtc gagttgcttt tcggggggcg ggccgggggc ggaggcgggc
     3241 gcggcggcgg gggcggaggc aaccatgttg tcgggggaga ggttcaaagt ggtcacggcg
     3301 gggcggcggc gcgcgaggag cgcggggacg aggtggaacc actggacata gccgagggcg
     3361 agcgccatca tccagagcag gatgcgttcg ccgatgccgc ccgtgcccgt gtagaaggcg
     3421 tacatgaaga ggctgccgag cggaaacgag agcacgaaca tcaaaagttc gagccgcgct
     3481 gtcagcgcgc cgaggtcgta gaggtcttcc tgccggacgg ggatgacggt gtagaggacg
     3541 aggacggtcg tgcacagaga cagccagtgt ttgcgcaggt agtgccggga taaatttcgc
     3601 atgctgcccc ctccgatctg tggcccgtgt gacttcggcg tccggcggcg tgagggaact
     3661 cagccttccg tcagcatcac ccacgaagcg cgcaggaaat cgactggatg atcggctatc
     3721 gagaccacgt aacacttcca cgacgtgcga aagcattgtg tcctgccggg gtcatccggc
     3781 ctgcgcacgt aggcgtcgca cgcggcgccg agggcgaccg ccaacgcgaa cggaagcaga
     3841 cgaagcttga gtagccccat gccgcttctc ccttccggtg tcgagcctta tagacaagcc
     3901 gccgccgctt ttgttccacg attctcgcag gccgcaagaa agagcaccgg gggcgttcta
     3961 caatctgggc gggcggcgtg ccgccgcgct tcgaaatgat gacgggaaga aagcttctgt
     4021 gagggaacga atgatgcgaa ccgtgagaga tttgctgtgc ctcgttttcg gcgtcctgct
     4081 cctggcgctc gcgggggcgg cgaccgccaa cgcgcaaggc gtgatcgtgc ccggcccgtg
     4141 ccgccgctgc cccgtgccgc cgcggcccat cgctttgccg cgcgcgctgc ccgtcaagtc
     4201 cgtcaagatc gagacgaa
//
LOCUS       DBNGOH010000311         4603 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1880802, whole genome shotgun sequence.
ACCESSION   DBNGOH010000311 DBNGOH010000000
VERSION     DBNGOH010000311.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4603)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4603)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4603
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1880802"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..388
                     /locus_tag="AC159P_05990"
     CDS             <1..388
                     /locus_tag="AC159P_05990"
                     /inference="COORDINATES: protein motif:HMM:NF015780.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HIR3695040.1"
                     /translation="TLDAYRRGAHAAEVPALRMLAATREEIEQRARALADRLRARPAS
                     AALAFDIVEGASAVGGGSAPTTHPPTALLAVTHDALSASALEEALRARRPPVVARILG
                     DRVVLDLRTVSESEEPELLEALLSIT"
     gene            574..1767
                     /locus_tag="AC159P_05995"
     CDS             574..1767
                     /locus_tag="AC159P_05995"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3695041.1"
                     /translation="MKRLISSLKNALGSLAAVRARRLSLFDSAGARIERKPLTEQSFT
                     ARVLAASTDGQAVAYLDGTLACLNDAMLEAARLTGEEARSLDLCSLLERFRAGVFDEP
                     ALAVRRVWQTGEAYERELSFADPSRTLALRIALITRGADDERTRRAQPKAPLAVAVTV
                     RDVTRIKEYERLKSDMLSLMSHELRTPITSINGFAELLVMDETLPAEAREFLSIINNE
                     SQRLARMINTFLSVTKLEQKDSQEVWMAPVMLDDVVREAIDIYQPVADRKRIRLVERD
                     GVRLPPVAADRGMIAQVVGNLVDNAIKYSPERTAVTLSATLEADAVRLTVEDRGYGIP
                     PEDLARVWEKFYRVARDGHPKEGESTGLGLSFVREVVEQHGGAVSLESELGHGSRFSF
                     TLPRL"
     gene            complement(1860..2276)
                     /locus_tag="AC159P_06000"
     CDS             complement(1860..2276)
                     /locus_tag="AC159P_06000"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013052.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein"
                     /protein_id="HIR3695042.1"
                     /translation="MPLAPGLTISKSLITGKGCFATRLFPKGRKIAEYTGERITHREA
                     ARRIRTRKRHRVCALDRRWSIDGSRGGNGLHYVNHSCAPNSYMRTTRGHLLFIALRDI
                     LPGEEITCDYVTTYHPDDYKCRCKAPTCRGTINKKP"
     gene            complement(2398..2904)
                     /locus_tag="AC159P_06005"
     CDS             complement(2398..2904)
                     /locus_tag="AC159P_06005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695043.1"
                     /translation="MRRFSTLSAIALSLVFSCAAALGQETFENAAYSLELPNATWKAT
                     ARSDNVHQHTEFIYGDRLDGHLRLRREVVEAEMTPEKLARRDEDQKLRYQNGFVGGKL
                     TPFAGRLNGSVLAYEYTAAGKPMAGRVYYLQADPRTIYVLQFTGLRDKLLRIQNQTDS
                     IARSFKLK"
     gene            complement(3163..3927)
                     /gene="rph"
                     /locus_tag="AC159P_06010"
     CDS             complement(3163..3927)
                     /gene="rph"
                     /locus_tag="AC159P_06010"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006025617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HIR3695044.1"
                     /translation="MSFTPAKGDFKRTDNRRADELRRVLITPHYMPYAEGSALIEMGG
                     TRVICTATLEDRVPPFRRNTGQGWLTAEYAMLPRATAQRTQRETGRGGPSGRTHEIQR
                     LIGRSLRAVADLSALGERTVTLDCDVLQADGGTRTASITGAYVAFALACHRLAKDGKI
                     TRLPLKGEVAAVSVGIVGATPLLDLKYDEDSRAAVDMNVVCTGDGRFIEVQGTAEGEA
                     FSREQMDSLLELARLGLEELFAAQRAAIEAGKAATG"
     gene            complement(3908..>4603)
                     /gene="murI"
                     /locus_tag="AC159P_06015"
     CDS             complement(3908..>4603)
                     /gene="murI"
                     /locus_tag="AC159P_06015"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HIR3695045.1"
                     /translation="EARGVKILVVACNTASALALPAIRASVKVPVVGMIGPGARAAVS
                     AAGDAGRVGVIATESTVRSGAYREAIQRLAPGAEVVERACPLFVSLAEEGWAETDVAR
                     KVAEEYLGDLRGQQLGALVLGCTHYPILRRVIQEAVGEGVRQIDSGEAAAEDVEALLE
                     TEGLRRREYNTATGKLLMERARAGRLCDDLDHFYVTDAAERFARVAERFLGATPRVLE
                     AVEVWGHDELHTR"
BASE COUNT          699 a         1627 c         1523 g          754 t
ORIGIN      
        1 gacgctcgac gcctaccgcc gcggcgcgca cgcagcggaa gtccccgcgc tccgcatgct
       61 cgccgcgacg cgcgaagaga tcgaacaacg cgcacgcgcc ctcgccgacc gcctgcgcgc
      121 gcgccccgcc tccgccgccc tcgcgttcga catcgtcgaa ggcgcgtccg ccgtcggcgg
      181 cggctccgcc ccgaccacgc acccgccgac cgcgctcctc gccgtcacgc acgacgctct
      241 ttcggcgagc gcgctcgaag aagccctgcg cgcccggcgc ccgcccgtcg tcgcgcgcat
      301 cctgggcgac cgcgtcgtcc tcgacctgcg gacggtctcc gaaagcgaag agccggagct
      361 gctcgaagcg ctgctctcga tcacatgacg cggcccgccc ggcggcggcg cgcgcgatgc
      421 cctgcacacc atcacttgac cgccgagcac gacgcctctc aagaatcatc gcttgtccgt
      481 ccggcgtctc gcgttgtatg attcttcgcg tcgcaggcgc ctcaaccgcc gcgtccgttc
      541 gaaaccttcc ttcgcttgcc ctctcactta accttgaaga gattgatttc ctctctgaaa
      601 aacgcgcttg ggtcgctcgc tgccgtgcgg gcgcggcggc tgtcgctgtt cgactcggcg
      661 ggcgcgcgga tcgagcgcaa gccgctgacg gagcagagct tcacggcgcg cgtcctcgcc
      721 gcttcgaccg acgggcaggc ggtcgcctac ctcgacggca cgctcgcctg cctcaacgac
      781 gcgatgctcg aagccgcgcg gctcacaggc gaagaggcgc gctcgctcga cctctgcagc
      841 ctgctcgaac gcttccgcgc gggcgtcttc gacgaacccg cgctcgccgt gcgccgcgtc
      901 tggcagaccg gcgaggctta cgagcgcgag ctttccttcg cagaccccag ccgcacgctc
      961 gcgctccgca tcgcgctcat cacccgcggc gccgatgatg aacgaacacg acgcgcgcag
     1021 ccgaaggcgc cgctcgccgt cgccgtcacc gtgcgcgacg tgacgcgcat caaggagtac
     1081 gagcggctga agagcgacat gctctcgctc atgtcgcacg agctgcgcac gcccatcacc
     1141 tcgatcaacg gcttcgcgga actgctcgtg atggacgaga cgctgcccgc cgaggcgcgc
     1201 gagttcctct ccatcatcaa caacgagtcg cagcgcctcg cgcgcatgat caacacgttc
     1261 ctctccgtca cgaagctcga acagaaggac agtcaggagg tctggatggc gcccgtgatg
     1321 ctggacgacg tggtgcgcga ggcgatcgac atctaccagc ccgtcgccga caggaagcgt
     1381 atccgcctcg tcgagaggga cggcgtgcgc ctgccgcccg tcgccgccga ccgcggcatg
     1441 atcgcgcagg tcgtcggcaa cctcgtggac aacgccatca agtacagccc cgagcgcacc
     1501 gccgtcacgc tctcggccac gctcgaagcc gacgccgtgc gcctcacggt cgaagaccgc
     1561 ggctacggca tcccgcccga agacctcgcg cgcgtctggg agaagttcta ccgcgtcgcc
     1621 cgcgacggcc acccgaagga gggcgaatcg accggcctcg gcctctcctt cgtccgcgaa
     1681 gtcgtcgaac agcacggcgg cgccgtctcc ctcgaatccg aactcggcca cggctcccgc
     1741 ttcagcttca cgcttcccag actctaaaag ccgtgaaccg gcaaaagccg taagccgtga
     1801 accgtaaacc gtgaacagtc agaaccggtt ttttcctatc acggtttacg gttcacggct
     1861 tacggctttt tgttgatggt gcccctgcac gtcggcgctt tgcagcggca cttgtagtcg
     1921 tcggggtggt aggtggtgac gtagtcgcag gtgatctctt cgccggggag gatgtccctg
     1981 agcgcgatga agaggaggtg gccgcgcgtc gtgcgcatgt agctgttggg cgcgcaggag
     2041 tggttgacgt agtggaggcc gttgccgccg cggctgccgt cgatgctcca ccggcggtcg
     2101 agcgcgcaga cgcggtggcg cttgcgcgtg cggatgcggc gcgcggcctc gcggtgcgtg
     2161 atgcgctcgc ccgtgtactc ggcgatcttc ctccccttcg ggaacagccg cgtggcgaag
     2221 cagcctttgc cggtgatgag agacttgctg atcgtgagtc cgggggcgag aggcataaag
     2281 cagtaggcag caggcagcgg gcagcaggca gtcaagaagt ctcgttaatg cagcgtcagg
     2341 atgtacacgt caacgccgct gtgctgagac tgcctgctgc ccgctgccta ctgcctgcta
     2401 tttcagcttg aagctgcggg cgatggagtc ggtctggttc tggatgcgga ggagcttgtc
     2461 gcgcaggccg gtgaactgga gcacgtagat ggtgcggggg tctgcctgaa ggtagtagac
     2521 gcgcccggcc atcggcttgc cggcggcggt gtactcgtag gcgaggacgg agccgttcag
     2581 gcgaccggcg aagggcgtga gcttgccgcc gacgaagccg ttctggtagc ggagcttctg
     2641 atcctcgtcg cggcgggcga gcttctcggg cgtcatctcc gcttcgacga cctctctgcg
     2701 caggcggagg tggccgtcca gacggtcgcc gtagatgaac tccgtgtgct ggtggacgtt
     2761 gtcggagcgc gcggtggcct tccacgtcgc gttcggcagt tcgagcgagt aggcggcgtt
     2821 ctcgaaggtc tcctgcccga gcgctgcggc gcaggagaag acgagcgaca gggcgatggc
     2881 ggacagggtg ctaaaacgtc gcatgaccgg ggattcactc ctctgaagtt agagcctcga
     2941 acgggaagta cggagcgtcg tgcgtcaact ctctgttcgg cttcagcctc acaatcagat
     3001 gccttccgcg cggcccattg ttgcccgtcc ccgtgccagt ttgcaacggt gaaaacggtg
     3061 aaagcggacg gggagacaag gagacggggc gacggggaga aaatgaattc gcgatgtcga
     3121 atgactgctt tatctctttc gccccgtcgc cccgtctccc cttcaccccg tcgccgcttt
     3181 ccccgcttcg atggcggcgc gttgcgcggc gaacagctct tcgaggccga ggcgcgcgag
     3241 ttcgaggagg ctgtccatct gttcgcgcga gaacgcctcg ccctcggcgg tgccctgcac
     3301 ttcgatgaag cgcccgtcgc ccgtgcagac gacgttcatg tccacggcgg cgcgcgagtc
     3361 ctcgtcgtac ttgaggtcca ggagcggcgt cgcgccgacg atgccgacgc tgacggcggc
     3421 gacctcgccc ttcaaaggca gccgcgtgat cttgccgtcc ttcgcgagcc ggtggcaggc
     3481 gagcgcgaag gcgacgtagg cgcccgtgat cgaggccgtg cgcgtgccgc cgtcggcctg
     3541 tagcacgtcg cagtcgagcg tgacggtgcg ctcgccgagg gccgagaggt ccgcgacggc
     3601 gcgcaggctc cgaccgatga gccgctgtat ctcgtgcgtg cgcccggagg ggccgccgcg
     3661 gcccgtctcg cgctgcgtgc gctgcgcggt ggcgcgcggc agcatcgcgt actcggcggt
     3721 gagccagccc tgccccgtgt tgcggcggaa cgggggcacg cggtcttcga gcgtggccgt
     3781 gcagatgacg cgggtgccgc ccatctcgat gagcgccgag ccttcggcgt agggcatgta
     3841 gtgcggcgtg atcagtacgc gccgcagctc gtcggcgcgg cggttgtccg tcctcttgaa
     3901 atcgccttta gcgggtgtga agctcatcgt gcccccagac ctccacggct tccagcacgc
     3961 gcggcgtcgc gcccaaaaac ctctcggcga cgcgcgcgaa acgctccgcc gcgtccgtca
     4021 cgtagaagtg atcgaggtcg tcgcacagcc tgcccgcgcg cgcgcgctcc atcaacagtt
     4081 ttccggtcgc cgtgttgtac tcgcgccgcc gcaacccctc cgtttcgagc agcgcctcga
     4141 cgtcttccgc cgccgcctcg cccgaatcga tctggcgcac gccctcgccc acagcctcct
     4201 gtatcacgcg gcgcaagatc ggatagtgcg tgcacccgag aaccagcgcg ccgagctgct
     4261 ggccgcgcaa atcgcccaga tactcttccg cgaccttgcg cgccacgtcc gtctcggccc
     4321 aaccctcctc ggccagcgag acgaagagcg ggcacgcgcg ctcgacgacc tccgcgccgg
     4381 gcgcgagccg ctgaatcgct tcgcggtagg cgccgctccg cacggtcgat tccgtggcga
     4441 tgacgccgac gcggcccgcg tcgcccgccg cactcaccgc cgcgcgggcg cccggcccga
     4501 tcatgcccac caccggcacc ttcacgctcg cgcgaatcgc cggcagcgcc agcgccgacg
     4561 ccgtgttgca ggcgacgacc agaatcttca cgccgcgcgc ttc
//
LOCUS       DBNGOH010000312         3678 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_780138, whole genome shotgun sequence.
ACCESSION   DBNGOH010000312 DBNGOH010000000
VERSION     DBNGOH010000312.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3678)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3678)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3678
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_780138"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..893)
                     /locus_tag="AC159P_06020"
     CDS             complement(<1..893)
                     /locus_tag="AC159P_06020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695046.1"
                     /translation="MPPPGKEDEDVTTKPNDPRRASSIAWLAFCAACLCLFLDGGAAR
                     AQAQGACQPRVRVKTLLDNLQVGGRGALSIGKLYAECLPEPVRRSQGNYGYNPYDGGK
                     LSAVLKASNGQPLNTYVFYAEKITSMWELSRYEVVGGPQAVKPLAVGSYSLDFAIEDK
                     VFQRFPFSVSTVESKDVYRPETIYLLDGPWSDYAELYYPKPDRYTQLWVWLRDRENAG
                     AQLREIQYTLRLVRERDGKLIAEGGGATSAMRLRPQWVGYKLSFRPLSVPAGGSSEFH
                     ASGLLASEGDHRIELSLGGKP"
     gene            1120..1827
                     /locus_tag="AC159P_06025"
     CDS             1120..1827
                     /locus_tag="AC159P_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HIR3695047.1"
                     /translation="MAGILSRLGNLWRGFLRLWISDVEKEHPEIAYENAIDSMIEKYS
                     RLKTATAAIIRRREETDERFKRAAAELAQTEAELDTAVDTNQDDLALILIQKKNALTA
                     EVGELKAEMDTAQKDADSAKQSLLGVQSQIRKLQSEKDSMLAKMQSAQARIKVQEQLD
                     GLSVDAEVKALDNVRQHIKTTIAEANLGKELSESSLDNRLAALKSQVGDVQAKNQLAE
                     LKARKAAQQASQGNKTM"
     gene            1954..>3678
                     /locus_tag="AC159P_06030"
     CDS             1954..>3678
                     /locus_tag="AC159P_06030"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012234.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR3695048.1"
                     /translation="MSDPRLLPHWAEDLRRRYLRGEASMFVLHGNVYDAVLHRGRILS
                     LTEFLTDVLLKESRETVVVYNVSTGARFAKRAPDITVHEELLLAPQKDRVLAALESIA
                     VTSSKAAVILEYAETIAPAGDPNFQADTDRASVVTLHRWSSLPEIERRDNIVLLVTEN
                     LTELSPKLVSNPKVAVVEVPMPDVETRKLAAQVANPKLSERDAERYAEITAGLKAVQI
                     ASILAPAPAAEEETREREEFIRKMLGAGSDAAERARKLAQLTSGMNPEEIKKLIAPDA
                     DPAAAEAEAGESAQERARKEADRLIARRKREILERECFGLVEFVEPNHGFEVVGGMDE
                     VKKDLLVIAESIREGRTSRVPMGVLFTGPMGTGKTFVAEAFAKECGLTTIKLKNFRSK
                     WVGATEGNLEKILGVIRAIGQVIVIIDEGDRAFGNTDGEGDGGTSSRVIARIKEFMSD
                     TANRGRILFLVMTNRPDKLDVDLKRAGRLDRKIPFLYSQTPEEVETVAAALVRKNKIK
                     TDVDFASIRDGFAAKLVGYSNADIEAVILMANDDAAREANDAAPESSVEAAVTAAHFQ
                     RAASDYFPS"
BASE COUNT          687 a         1267 c         1166 g          558 t
ORIGIN      
        1 tacggcttgc cgccgagcga gagttcgatg cggtggtcgc cttcggaggc gagcagcccg
       61 ctcgcgtgga attccgacga gccgcccgcg gggacggaga gcggcctgaa gctcagcttg
      121 tagccgaccc actgcggcct cagcctcatc gcggaggtcg cgccgccgcc ctccgcgatg
      181 agcttgccgt cgcgctcgcg cacgagccgg agcgtgtact gtatctcgcg gagctgcgcg
      241 cccgcgttct ccctgtcgcg cagccagacc cagagctgcg tgtagcggtc gggcttcggg
      301 tagtacagct cggcgtagtc tgaccacggg ccgtcgagca ggtagatggt ctcggggcgg
      361 tagacgtctt tactctcgac cgtcgagacc gagaagggga agcgctggaa gactttgtct
      421 tcgatggcga agtcgaggct gtagctcccg accgcgagcg gcttgacggc ctgcggcccg
      481 ccgaccactt cgtagcggct cagctcccac atcgaagtga tcttctccgc gtagaagacg
      541 taggtgttca aaggctgccc gttcgacgcc ttcaagaccg ccgagagctt gccgccgtcg
      601 taagggttgt agccgtagtt gccctgactc cggcgcacgg gttcgggcag gcactccgcg
      661 tagagcttgc cgatggagag cgcgccgcgc ccgccgacct gcaagttgtc gagcagcgtc
      721 ttcacgcgca cgcgcggctg gcacgcgccc tgcgcttgcg cgcgcgcggc gccgccgtca
      781 agaaagagac acaggcaggc ggcacagaat gcgagccacg cgatgctgct cgcgcggcgc
      841 gggtcgttcg gcttcgtcgt cacgtcttcg tcctccttac cgggtggcgg cacatgctat
      901 tcgatgccgc ggaactctgg caagaggcgg ggccgccccg gcatccgcct cccggcgcgc
      961 gctcgcccaa agaatcgctt cggcgcagcc gccgcgttgt gctagagtgt agcgccgtga
     1021 ttctaatccg ccccgtcgcc cgtgtcagcg gagtccgagt ctcctcggtt tcgagttcta
     1081 ttatcaacct gaaagctgaa tcgaaaggaa gcgaaggcaa tggcaggcat ccttagtcgt
     1141 ctgggcaact tgtggcgcgg cttcctgcgc ctctggattt ccgacgtgga gaaggagcac
     1201 ccggagatcg cctacgagaa cgccatcgac tcgatgatcg agaagtactc gcggctgaag
     1261 acggccaccg ccgccatcat ccgccgccgc gaagaaacgg acgagcgctt caagcgcgcc
     1321 gccgccgaac tcgcgcagac cgaggccgag ctggacaccg ccgtggacac caaccaggac
     1381 gacctcgcgc tgatcctgat tcagaagaag aacgcgctca ccgccgaggt cggcgaactc
     1441 aaggccgaga tggacaccgc gcagaaggac gccgactcgg ccaagcagtc gctgctcggc
     1501 gtccagtcgc agatccgcaa gctccagtcc gagaaggact cgatgctcgc caagatgcag
     1561 tccgcgcagg cgcgcatcaa ggtgcaggag cagctcgacg gcctctcggt ggacgccgag
     1621 gtgaaggcgc tcgacaacgt ccgccagcac atcaagacca ccatcgccga ggccaacctc
     1681 ggcaaagaac tctccgagtc ctcgctcgac aaccgcctcg ccgcgctcaa gagccaggtc
     1741 ggcgacgtgc aggccaagaa ccagctcgcc gaactcaagg cccgcaaggc cgcgcagcag
     1801 gcttcgcagg gaaacaaaac gatgtagtag gcagtaggca gcaggcagta ggcagtaggt
     1861 tgatagaagc gggcgctgct tcttaaccca gaccgccaaa ccaacgaagg ttgcctactg
     1921 cctactgcct actgcctact gcctactctt cttatgtctg atccccgctt gctcccccac
     1981 tgggccgaag acctgcgccg ccgctacctg cgcggcgagg cgtcgatgtt cgtgctccac
     2041 ggcaacgtct acgacgcggt cttgcaccgc ggcaggattc tctcgctgac ggagtttctg
     2101 accgacgtgc tcctgaagga gtcgcgcgag acggtcgtcg tctataacgt ctcgaccgga
     2161 gcgcgcttcg ccaagcgcgc gcccgacatc accgtccacg aagagctgct gctggcgccg
     2221 cagaaggatc gcgtgctggc tgctctcgaa agcatcgccg tcacctcttc gaaggcggcg
     2281 gtcatcctcg aatacgccga gaccatcgcg cccgcgggcg acccgaactt tcaggccgac
     2341 acagaccgcg cctcggtcgt cacgctccac cgctggtctt cgctcccgga gatcgagcgc
     2401 cgcgacaaca tcgtcctgct cgtcaccgaa aacctcaccg agctgtcgcc gaagctggtc
     2461 tcgaacccga aggtcgcggt ggtcgaggtg ccgatgcccg acgtggagac gcgcaagctc
     2521 gccgcgcagg tcgccaaccc gaagctctcc gagcgcgacg ccgagcgcta cgccgaaatc
     2581 accgcgggcc tgaaggccgt gcagatcgcc tccatcctcg cccccgcgcc cgccgccgaa
     2641 gaggagacgc gcgagcgcga ggagttcatc cgcaagatgc tcggcgcagg ttcggacgcc
     2701 gccgagcgcg cccgaaagct cgcgcaactg acgagcggca tgaaccccga ggagatcaaa
     2761 aaactcatcg ccccggacgc cgacccggcg gcagccgagg cagaggcggg cgagtcggcg
     2821 caggagcgcg cgcgcaaaga ggccgaccgt ttgatcgcgc ggcgcaagcg cgagattctg
     2881 gagcgcgagt gcttcggcct cgtcgagttt gtcgagccca accacggctt cgaggtcgtc
     2941 ggcgggatgg acgaggtgaa gaaagacctg ctcgtcatcg ccgaatcgat ccgcgagggg
     3001 cgcaccagcc gcgtcccgat gggcgtcctc ttcaccggcc cgatgggcac gggcaagacc
     3061 ttcgtcgccg aagcctttgc caaagagtgc ggcctcacca ccatcaagct caagaacttc
     3121 cgctcgaagt gggtcggcgc gaccgagggc aacctcgaaa aaatcctcgg cgtcatccgc
     3181 gccatcgggc aggtcatcgt catcatcgac gagggcgacc gcgccttcgg caacaccgac
     3241 ggcgagggcg acggcggcac ctcctcgcgc gtcatcgcgc gcatcaagga gttcatgtcc
     3301 gacacggcga accgcgggcg catcctcttc ctcgtcatga ccaaccgccc cgacaagctc
     3361 gacgtggact tgaagcgcgc gggccgtctc gaccgcaaga ttccgtttct ttattcgcag
     3421 acgcccgagg aggtcgagac ggtcgccgcc gcgctcgtgc gcaagaacaa gatcaaaacg
     3481 gacgttgact tcgcctcgat ccgcgacggc ttcgccgcga agctcgtcgg ctacagcaac
     3541 gccgacatcg aggccgtcat cctgatggcc aacgacgacg cggcgcgcga agccaacgac
     3601 gccgcgcccg agtcgagtgt cgaggcggca gtcactgccg cgcacttcca gcgcgccgcc
     3661 tccgactact tcccgagc
//
LOCUS       DBNGOH010000313         3942 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4262691, whole genome shotgun sequence.
ACCESSION   DBNGOH010000313 DBNGOH010000000
VERSION     DBNGOH010000313.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3942)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3942)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3942
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4262691"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..609)
                     /gene="sat"
                     /locus_tag="AC159P_06035"
     CDS             complement(<1..609)
                     /gene="sat"
                     /locus_tag="AC159P_06035"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: protein motif:HMM:NF025664.7"
                     /note="ATP sulfurylase; ATPS; converts ATP and sulfate to
                     5'phosphosulfate and pyrophosphate; in some organisms this
                     enzyme is involved in the incorporation of inorganic
                     sulfate while in others it is involved in the production
                     of ATP in the reverse direction; the enzyme from Thermus
                     thermophilus is dimeric and binds a zinc ion that is
                     coordinated by cysteine and histidine residues that are
                     not found in all related proteins but is found in some
                     thermophilic organisms; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase"
                     /protein_id="HIR3695049.1"
                     /translation="MRLISPHGGALVNREVAGTERERLLEAAKGMPALRLGARAVADL
                     ELIATGAYSPLEGFMGEADYLSVRDWMQLADGVAWSLPITLSVTAGEAAALREGSDVA
                     LYEGGHFVGVLHLAEKFARDREREAEQVYRTTDAAHPGVRALYAEGEWLLGGRVSLIN
                     RPLERAFGAYRRDPAETRALFRERGWRRVVAFQTRNPVHRAHE"
     gene            complement(748..1467)
                     /locus_tag="AC159P_06040"
     CDS             complement(748..1467)
                     /locus_tag="AC159P_06040"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012526138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HIR3695050.1"
                     /translation="MTATALTLGHEATDEELKIISDGFEGAAPDVVLRWALEEFGDDV
                     ALATGFGVEGCVLVDALARVNRGARIFYLDTDLLFPDTYALIERLRARYGVRFERRAT
                     RLSLEAQAARHGDRLWEHDPAECCRLRKVEPLRETLAGLRAWVTAIRRDQTPARAGAG
                     LVERDAKFGLVKINPLAAWSSRDVWRYVSQHEVPYNPLHDLGYPSVGCAPCTTLVKIG
                     EDPRAGRWRGTAKTECGIHSG"
     gene            complement(1481..2734)
                     /gene="cobA"
                     /locus_tag="AC159P_06045"
     CDS             complement(1481..2734)
                     /gene="cobA"
                     /locus_tag="AC159P_06045"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: protein motif:HMM:NF004790.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HIR3695051.1"
                     /translation="MADGVSGAARRGVLVVGHGSRREEANEDVRAAARRIAARGGFEL
                     TQAAFLEIEHPNVAAGFARLVERGARSVTVHPYFLSPGRHTRGDLPVEVREAAARHPG
                     VTYRITEPLSAHRLVIEASIERIRETEGRATSEAEGWKTGEPLSRNPSHAARRGTVYL
                     VGAGPGDPGLLTLKARDLLASCDVVVYDYLVNPEILRHVASHAERVYVGKVGGGRQTP
                     QDEINRTLVAHARGGASVVRLKGGDPFLYGRGGEEAEALGAAGVAFDVVPGISSALAV
                     PAYAGIPLTHRGLSASVAVVTGTRAEESDGSRSLASSAAADTVVVLMGVSRLREIAAE
                     LVAAGRSPETPAAVVRWGTYEGQQTVTGTLRTIGDEAERAAMRAPAVIVVGEVVSLRE
                     RLRWFESTQTFEAENDLEMAAVSAV"
     gene            complement(2719..>3942)
                     /locus_tag="AC159P_06050"
     CDS             complement(2719..>3942)
                     /locus_tag="AC159P_06050"
                     /EC_number="1.8.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013259.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HIR3695052.1"
                     /translation="GACGDITRNVTGCPLAGIVAAEFIDAEPLALDVHNHFLDNREFS
                     NLPRKFKITVSGCSLYCTGHEIADIGLVALRRADGEVVFDLWVGGGLGSKERFADRLG
                     AHVRAHEAVEVCAHICALFRDHGNRDSRARARLKFLLGEWGPRRFREELERRLGRALP
                     DGEAPAVPVTANRAHVGIHPQKQRGLYYAGVATKRGRLPGRDMSAVAELARRHGGGRV
                     RLTTSQNLVVLDVPEAERVALAVALRTLDLQVVASSFRTGTVACTGKQFCKLAVTETK
                     ARASELIEHLERALPGFNVPLRISVTGCPNSCAHYQVCDIGFVGDFLNTPGGKREAYR
                     VYLGGHLGDGHTFGRELQKKIPAEEIKHYVERLARTYIERRNGAGDSFQAFIARHTTE
                     ELETLGEVEELWLTA"
BASE COUNT          591 a         1391 c         1357 g          603 t
ORIGIN      
        1 ctcgtgcgcg cggtggacgg ggttgcgcgt ctggaaggcg acgacgcgcc gccacccgcg
       61 ctcgcggaac agcgcgcgcg tctccgcagg gtctctgcgg taagcgccga aggcgcgttc
      121 aagcgggcgg ttaatcaggc tgacgcgccc gccgagcagc cactcgccct ccgcgtaaag
      181 cgcgcgcacg cccggatgcg ccgcgtccgt cgtgcgatag acctgctcgg cctcgcgctc
      241 cctgtcgcgc gcgaacttct ccgcgaggtg aagcacgccg acgaagtgcc cgccctcgta
      301 gagcgccacg tcgcttcctt cgcggagcgc ggcggcctcg cctgccgtga cggagagcgt
      361 gatcggcaga gaccacgcga ccccgtccgc gagctgcatc cagtcgcgca ccgacaggta
      421 atcggcctcg cccatgaaac cttcgagcgg cgagtaggcg cccgtcgcga tcaactccaa
      481 atccgccacc gcgcgcgcgc cgagccgcaa cgcgggcatt cccttcgcgg cttccaacag
      541 ccgctccctt tccgtcccgg cgacttcccg gttaacgagc gcgccgccgt gggggctaat
      601 caatctcatg ttgtcctttc ttcgtagctc ttagctatta gctgttagct cttagctaaa
      661 accctgattc ggaaacttct cgattacaac aacggtctgc aatgcatcga gtcatcaagc
      721 taacagctaa tagctaatag ctaaccgcta accgctatgt atcccgcatt cggtcttggc
      781 cgtgccgcgc cagcgaccgg cgcgcgggtc ttcgccgatc ttgacgagcg tggtgcacgg
      841 ggcgcagccg acggacgggt agccgaggtc gtgcagcggg ttgtaaggca cttcgtgctg
      901 cgagacgtag cgccacacgt cgcgcgaaga ccacgcggcg agcgggttga tcttgacgag
      961 gccgaacttc gcgtcgcgct cgaccagccc cgcgcccgcg cgcgccgggg tctggtcgcg
     1021 gcggatggcc gtgacccatg cgcgcagccc ggcgagcgtc tcgcgcagcg gctcgacttt
     1081 acgcaggcgg cagcactccg cggggtcgtg ctcccacaac ctgtcgccgt ggcgcgcggc
     1141 ctgcgcttcg agcgagagac gcgtcgcgcg ccgctcgaag cggacgccgt agcgcgcgcg
     1201 cagccgctcg atgagcgcgt acgtgtctgg aaagagcaag tccgtgtcga gataaaaaat
     1261 ccgcgcgccg cggttgacgc gcgcgagcgc gtccacgagc acgcagccct cgacgccgaa
     1321 gccggtggcg agcgccacgt cgtcgccgaa ctcctccaaa gcccagcgca ggacaacatc
     1381 cggcgcggcg ccctcgaatc cgtccgagat tatcttcagc tcttcgtcgg tcgcttcgtg
     1441 tccgagcgtg agcgcggtcg cagtcatcga ctcatcccct tcagaccgcc gaaacagcgg
     1501 ccatctcaag gtcgttctcc gcctcgaagg tctgcgtgct ctcgaaccaa cgaagccgct
     1561 cgcgcagaga gacgacctcg ccgacgacga tgacggcggg cgcgcgcatc gcggcgcgct
     1621 cggcctcgtc gccgatggtg cggagcgtgc ccgtgacggt ctgctgcccc tcgtacgtgc
     1681 cccagcggac gaccgccgcg ggcgtctccg gcgagcgccc cgccgcgacg agttccgcgg
     1741 cgatctcgcg gaggcgcgag acgcccatca gcacgacgac ggtgtccgcc gccgctgaag
     1801 acgcgagcga gcgtgaaccg tcgctctctt cggcgcgcgt gcccgtgacg acggcgaccg
     1861 aagcggagag accgcggtgc gtcagcggga tgcctgcgta agccgggacg gcgagcgcgg
     1921 agctgatgcc gggcacgaca tcgaacgcga cgcccgccgc gccgagcgcc tcggcctctt
     1981 cgccgccgcg cccgtagagg aacgggtcgc cgcccttgag ccgcacgacg ctcgccccgc
     2041 cacgggcgtg cgcgacgagc gtgcggttga tctcgtcctg cggcgtctgc cgcccgccgc
     2101 cgaccttgcc gacgtagacg cgctccgcgt gcgaagcgac gtgtcgaagg atttccggat
     2161 tgacgaggta gtcgtagacg accacgtcgc acgacgcgag caggtcgcgg gccttcagcg
     2221 tcagcaggcc ggggtcgccc ggccccgcgc cgacgaggta gaccgtcccg cgcctcgccg
     2281 cgtgcgaggg gttgcgggac agcggctctc ccgtcttcca tccttcggcc tcgcttgtcg
     2341 cccgcccttc cgtttcgcgg atgcgttcga ttgaggcttc gatgacgagg cggtgcgcgg
     2401 agagcggttc ggtgatgcgg tatgtgacgc cggggtgtcg cgccgcggct tcgcggactt
     2461 cgacgggcaa atcgccgcgc gtgtggcggc ccggcgagag gaagtaaggg tggacggtca
     2521 cgctccgcgc gccgcgctcg acgagacgcg cgaagcctgc ggcgacgttc gggtgctcga
     2581 tctcaagaaa ggcggcctgc gtcagctcga agccgccgcg cgcggcgatg cgccgcgccg
     2641 ccgcgcgcac gtcctcgttc gcctcctcgc gccggctgcc gtggccgacg acgagcacgc
     2701 ccctccgcgc cgctccgctc acgccgtcag ccatagctct tcgacctccc cgagcgtttc
     2761 cagttcttcc gtcgtgtggc gcgcgatgaa cgcctggaag ctgtcgcccg cgccgttgcg
     2821 ccgctcgatg tacgtgcgcg cgaggcgctc gacgtagtgc ttgatctcct cagccggtat
     2881 cttcttttgc agctcgcgcc cgaacgtgtg gccgtcgccg aggtggccgc cgaggtagac
     2941 gcggtacgcc tcccgcttcc cgccgggcgt gttgagaaag tcgccgacga agccgatgtc
     3001 gcagacctga tagtgcgcgc aactgttggg gcagcccgtg acgctgatgc gcagcggcac
     3061 gttgaagccg ggcaatgcgc gttcgaggtg ttcgatcagc tcggaggcgc gcgccttcgt
     3121 ctccgtcacg gcgagcttgc agaactgctt gccggtgcag gcgacggtgc ccgtgcggaa
     3181 ggacgaggcg acgacctgca aatcgagagt gcgcagggcg acggcgagcg ccacgcgctc
     3241 cgcttcgggc acgtcgagca cgacgaggtt ctgcgaggtt gtcaaacgca cgcgtccgcc
     3301 gccgtgacgg cgcgcgagtt cggcgaccgc ggacatgtct ctgcccggca gccgaccgcg
     3361 cttcgtggcg acgcccgcgt agtacagccc gcgctgcttc tgcgggtgga tgccgacgtg
     3421 cgcgcggttg gccgtgacgg gaaccgcggg cgcctcgccg tccggaagcg cgcggcccag
     3481 acgcctttcg agttcttcgc ggaagcgccg aggcccccac tcgccgagca gaaacttgag
     3541 ccgcgcgcgg gcgcgcgagt cgcggttgcc gtggtcgcgg aagagggcac agatgtgcgc
     3601 gcagacctcg accgcctcgt gcgcgcggac gtgcgcgccg aggcggtcgg cgaagcgctc
     3661 tttcgagccg aggccgccgc cgacccacaa atcgaacacg acctcgccgt ccgcgcgcct
     3721 caaggcgacg aggccgatgt ccgcgatctc gtggccggtg cagtagagcg agcagcccga
     3781 gacggtgatc ttgaacttgc gcggcaggtt cgagaactcg cggttgtcga gaaaatggtt
     3841 gtgcacgtcg agggccagcg gctcggcgtc aataaactcc gccgcgacaa taccggcgag
     3901 cgggcaaccc gtgacgttgc gcgtgatgtc gccgcacgcg cc
//
LOCUS       DBNGOH010000314         2461 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_414669, whole genome shotgun sequence.
ACCESSION   DBNGOH010000314 DBNGOH010000000
VERSION     DBNGOH010000314.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2461)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2461)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2461
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_414669"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1773)
                     /locus_tag="AC159P_06055"
     CDS             complement(<1..1773)
                     /locus_tag="AC159P_06055"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF014869.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M4 family metallopeptidase"
                     /protein_id="HIR3695053.1"
                     /translation="MRTPQRPQRFWVKSSLISLLVLSLLALATGAIRVFDRPAEAAGA
                     TPPPQAGKKAAQETKAAPAASAALDKLRRASGAEIRAHVARETGHYDFVRAAGGGVLA
                     PAHAGSSPEERAAAFLREHGALVGLGDAERQALGGPASPLKTARVVEDAVGGQHVRLG
                     QTYRGLPVFGAQVVVHLNARGVTAVNGHFVPGLRLDTRPAVKQAAAEAAAVRERFGSA
                     PLSVVKTELAIYRVGLLEGFEGPSVLAYAVEVTDGGATRAQVWVDALKGTILNRISLN
                     HKALDRVIYTPIFSPVTAVRREGDPYTPGLPPGTTGADPINNLYLFAGETYNLWMSGF
                     GRDSYDGAGHTMHSVYLVNQVCPNAYWNSVSTNYCPDFDSDDVVSHEWGHAYTEYTHG
                     LIYAYQSGALNESYSDIFGESHDLLNTTDAEGGNNNAQPLPDGQRWQMGEDVPTLNAE
                     ALGILRDMWDPTRYGDPDKVSSPNYACGSDDGGGVHTNSGVPNHAFAMLVDGKTYNGV
                     TVQGIGFNRALAIYYRAMTVYQTPTTNFVQHADALEQSCADLLGSTPNQVLTGSPVGT
                     PSGETIDAAVCQQVSNAILAVEMRD"
BASE COUNT          353 a          803 c          884 g          421 t
ORIGIN      
        1 gtcgcgcatc tcgacggcga ggatcgcgtt cgagacctgc tggcagacgg cggcgtcgat
       61 ggtctcgccg gacggggtgc cgacgggcga gccggtcagc acctgatttg gcgtcgagcc
      121 tagaaggtcg gcgcaggact gctcaagcgc gtccgcgtgc tggacgaagt tggtggtggg
      181 cgtctggtag accgtcatcg cgcggtagta gatcgccagc gcgcggttga agccgatgcc
      241 ctggacggtc acgccgttgt aggtcttgcc gtccacgagc atggcgaagg cgtggttggg
      301 cacgcccgag ttggtgtgga cgccgccgcc gtcgtccgag ccgcaggcgt agttgggcga
      361 agagaccttg tcggggtcgc cgtagcgcgt cgggtcccac atgtcgcgca ggatgccgag
      421 cgcctccgcg ttcagggtcg gcacgtcctc gcccatctgc cagcgctggc cgtccggcaa
      481 aggctgcgcg ttgttgttgc cgccctccgc gtccgtggtg ttcaaaaggt cgtgcgactc
      541 gccgaagatg tccgagtagc tttcgttcaa agcgcccgac tggtaggcgt agatcagccc
      601 gtgcgtgtat tcggtgtagg cgtggcccca ctcgtgcgag accacgtcgt ccgagtcgaa
      661 gtccgggcag tagttggtcg agacgctgtt ccagtaggcg ttcgggcaga cctggttgac
      721 gaggtagacg ctgtgcatcg tgtggcccgc gccatcgtag gagtcgcgcc cgaagccgga
      781 catccacagg ttataggtct cgcccgcgaa cagatagagg ttgttgatgg ggtcggcgcc
      841 cgtcgtgccc ggcggcaggc ccggcgtgta ggggtcgccc tcgcggcgga cggcggtgac
      901 ggggctgaag atcggcgtgt agatgacgcg gtcgagcgcc ttgtggttga gcgagatgcg
      961 gttgaggatg gtgcccttca gggcgtccac ccagacctgc gcccgcgtcg ccccgccgtc
     1021 cgtcacctcg acggcgtagg ccagcacgct cggcccctcg aacccttcga gcagccccac
     1081 gcggtagatg gcaagctccg tcttgacgac gctcagcggc gcgctgccga accgctcgcg
     1141 caccgccgcg gcctctgccg ccgcctgctt gacggcgggg cgcgtgtcga gcctgaggcc
     1201 gggcacgaag tggccgttga cggcggtgac gccgcgcgcg ttgaggtgga caaccacctg
     1261 cgcgccgaag accggcaagc ccctgtaggt ctgcccgagg cggacgtgct ggccgccgac
     1321 cgcgtcttcg accacgcgcg ccgtcttgag cgggctggcc gggccgccga gcgcctgacg
     1381 ttcggcgtcg ccgaggccga ccagcgcgcc gtgttcgcgc aagaaagcgg cggcgcgctc
     1441 ttcgggagag gagccggcat gagccggggc aagcaccccg ccgcccgccg cccgcacgaa
     1501 gtcgtagtgg ccggtctcgc gcgcgacatg cgccctgatc tcggccccgc tcgcgcggcg
     1561 cagcttatcg agggcagcgc tggccgccgg cgctgccttg gtctcctgag ccgccttttt
     1621 gccggcttgc ggcggcggcg tggcgcccgc ggcctcggcg ggacggtcga aaacccgaat
     1681 cgcgcccgtc gcgagcgcga gtagactgag aaccagcagg ctgatcaagc tcgatttaac
     1741 ccagaaacgc tgcgggcgtt gcggtgtgcg catctcgtaa tctcctttat gacggtaagc
     1801 accgggtctt cgcgtccggc tcatttcgcg aggcagtcga tacggcatcg tctcggcatt
     1861 ccctgttctc acgttgccgc gcaatctacg ccccgccggt tgaggaaaag ttgagcctga
     1921 ctgtctcaac caaccgccgc gcccttttgg cccgccccgg cctcttccga cgggccggaa
     1981 ccgcttgaaa aaccgcccgc cgcgccccgc gagaagaccc cgccacgaaa ttttttctcc
     2041 cggctcaagt tttcctcaag tggccccgcc tatcctcgca acatcaaggc ggggggggaa
     2101 gtcactcgcc gggtgggcgt cgcagtcggg acggcgccgc cgggcgggcg accgggcggt
     2161 tcggagggcg gcttgccagg cagagacggg tcgccctccg agccgccatt cgatagggcg
     2221 tcggcgcgac tatgcaggct cccgcaaaag tcggttcggg agccgaccgg acattaaggt
     2281 ttcgcttccg gagcaggtgc tcggagggtg ttcaggcagt tgtaggactc acggacagga
     2341 gtgcggcccg caccagcgaa gggggcgaca aggcccgcgg ctatgcgtcg cgggccttgc
     2401 cgttttcccg ggcggggccg tgcgttctgt tagagcgttt tgtggttgag tttaccgcgc
     2461 g
//
LOCUS       DBNGOH010000315         4430 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3713590, whole genome shotgun sequence.
ACCESSION   DBNGOH010000315 DBNGOH010000000
VERSION     DBNGOH010000315.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4430)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4430)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4430
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3713590"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            88..804
                     /locus_tag="AC159P_06060"
     CDS             88..804
                     /locus_tag="AC159P_06060"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR3695054.1"
                     /translation="MSDLRILLWDIDGTLIRSVRPGGFKEYTIPVMESVFGTAGRLPE
                     MTVSGMTDLQIVAEALRDEGFTRVEMRRRVRELRARYLAELERVTAAQGAQMFEVLPG
                     VRAALAAADAHPRYRSALLTGNIEPAARLKMRLVGLSEYFALPGAFGDDSHDRRNLPR
                     LARERISRHLGFRLQPHQLVVIGDTPNDIACARHIGARVVGVGTGRVHGLDTLRPHAP
                     DVLLPDLSDTEAFMHALESL"
     gene            964..1320
                     /locus_tag="AC159P_06065"
     CDS             964..1320
                     /locus_tag="AC159P_06065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695055.1"
                     /translation="MLKRFSRRCLVIAALAGAVLFPAAAHAQRRDANRCRVGINGHQH
                     HQQARIRQGVRSGELTRRETARLQAEQARIRVQEAFARRSGGEFTLRERRRIAHEQQQ
                     ASRHIYRQKHDGQDRN"
     gene            complement(1409..3535)
                     /gene="mutL"
                     /locus_tag="AC159P_06070"
     CDS             complement(1409..3535)
                     /gene="mutL"
                     /locus_tag="AC159P_06070"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HIR3695056.1"
                     /translation="MSKIRVLSDHVANQIAAGEVVERPASVAKELVENAIDAGARRVE
                     IDSEGGGRRLLRVADDGEGMTRDDAVLAFERHATSKINTTEDLDRIGTLGFRGEALAS
                     IASVARVELVTQREGEAEGTRVLIEGGRMRDVAPAARPRGTTVAARDLFFNVPARRKF
                     LRSEATENYHLTNLVTHYALARPDIAFTLTSNGREILRAAPAADLRERAYQIFGAEFV
                     ESLLEVKGAHAGVARVRGYVSAPRERRTTRDAQFLFVNGRYVRDRVMTRALSEGFRSV
                     LPQGAFPAALLFLDVPPEEVDVNVHPAKTEVRFRRTSAIADAVRDAVRDALVAGGYLR
                     TTEDPDARDESPHRATRDDANLHDSPHAHDAHDARPASSTESADAFRRTDTASFGSES
                     HITSRAGREEARQESIEFEWPTTTDDSLWRDDARHAERPAARDSSNEDAATADDKASM
                     PTEAAIPEDTTTPRGPQAPGTSSSTRDTSSTGPAPSPSTSARAAGGALPPLGSAAGLV
                     REASAESLSADIRPLGQLADTYVVAADAEGLLLIDQHLAHERILFDKYRLLESGRAPE
                     SQNLLVPETFDLTPAQAAVFASVSEELERYGFSLMRLSGRTVAVRAVPADLPAGEARN
                     LLADIVGTTDSARRVRARDALREEIAASLANRAAVKGNTPLTPDKMRWLIDRLLRTSS
                     PTTSPNGRPIILRLARRDIERGFHRP"
     gene            complement(3663..>4430)
                     /gene="bshC"
                     /locus_tag="AC159P_06075"
     CDS             complement(3663..>4430)
                     /gene="bshC"
                     /locus_tag="AC159P_06075"
                     /inference="COORDINATES: protein motif:HMM:NF048910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis protein BshC"
                     /protein_id="HIR3695057.1"
                     /translation="IHDERGARHALARNAEGRYAAKGGAEEWTLDELADLAAREPERF
                     SPNVTLRSVVQDFMLPTIAYYGGAAEIAYFAQTNEVYRLLERPATPILHRASLTVVER
                     RTGRTLERYGLGIEDFFAGLDAVTARVVEEHLGADAARAFDETEEAIGRALETLRERL
                     THFDPTLGDALKTGRRKVEYQLHGLRSRFHRSQMARDRALFRQLERAATALYPEKALQ
                     ERHLNVTSLLARHGAYVVEWIHDAINIGTTDHQVVYL"
BASE COUNT          655 a         1400 c         1641 g          734 t
ORIGIN      
        1 cagtagcgct ccgcgtgatg tctgctgacg ctgacgcccg acattcggga gcgaaggttt
       61 ttctactgcc tactggctac ttgaatcatg tccgaccttc gcatcctcct ctgggacatt
      121 gacgggacgt tgatccgctc ggtgcgtccg ggcgggttca aggagtacac gatccccgtg
      181 atggagagcg tgttcgggac ggcggggcgt ttgccggaga tgacggtgtc ggggatgacc
      241 gacctgcaaa tcgtcgccga ggcgctgcgc gacgagggct tcacgcgcgt ggagatgcgg
      301 cggcgcgtgc gagagctgcg cgcgcgttac ctcgcggagc ttgagcgcgt gacggcggcg
      361 cagggcgcgc agatgttcga agtgctgccc ggcgtgcgcg cggcgctcgc ggcggcggac
      421 gcgcacccgc gctaccgctc ggcgctcctg acgggcaaca tcgaacccgc cgcgcgcctg
      481 aagatgcggc tggtcggact ctccgaatac ttcgccttgc ccggcgcctt cggcgacgac
      541 tcgcacgacc gccgcaacct gccgcgcctc gcgcgcgaac gcatcagccg ccacctcggc
      601 ttcaggcttc agccgcacca gctcgtcgtc atcggcgaca cgcccaacga catcgcctgc
      661 gcgcgccaca tcggcgcgcg cgtcgtcggc gtcggcacgg gccgcgtcca cggcctcgac
      721 acgctccgcc cgcacgcgcc cgacgtgctc ctcccagacc tctccgatac cgaagccttc
      781 atgcacgctc tcgaatcact ctgaagcccc gccttcgtcg aaacaaggcg ggcgcgcccg
      841 cggatttttt cagactttgc gccgccgcgt ttcgtcttaa ccgccgacct cgccactctc
      901 cctgaacaat caagatgctc ggcgcaagga gcgtcacgac ttgaacgcaa cgggaggcat
      961 ttcatgttga agagattttc gcggaggtgt ttggtcatcg cggcgctcgc gggggccgtg
     1021 ctctttccgg cggcggcgca cgcgcaacgg cgcgacgcga accgctgtcg cgtcggcatc
     1081 aacgggcacc agcaccatca acaggcgcgc attcgtcagg gcgtccgcag cggcgagttg
     1141 acccgccgcg agaccgcgcg ccttcaggcc gaacaggcac gcatcagggt tcaggaagcc
     1201 ttcgcccgcc gctccggcgg cgagttcacc ctgcgcgaac gccgccgcat cgcgcatgaa
     1261 cagcagcaag ccagtcgcca catctacagg cagaagcacg acgggcaaga ccgcaactga
     1321 agaaggggcg aaaaggggaa gaggggaaaa ggcgaaaagg gaagaaccgg ttttgtttta
     1381 cccttttccc cttttcccct ctctttcttt acggtcggtg gaagccgcgc tcaatgtctc
     1441 gtctggcgag gcggaggatg atggggcgac cgttgggaga ggtggtgggc gatgaggtgc
     1501 ggagcaggcg gtcgatgagc cagcgcatct tgtcgggggt gagcggcgtg ttgcctttga
     1561 cggcggcgcg gttggcgagc gaggcggcga tctcttcgcg cagcgcgtcg cgcgcgcgga
     1621 cgcgccgcgc ggagtcggtg gtgccgacga tgtctgcgag caggttgcgc gcttcgcccg
     1681 cggggaggtc tgccgggacg gcgcgcacgg cgacggtgcg gcccgacaga cgcatgagcg
     1741 agaagccgta gcgctcaagc tcttcggaga cggaggcgaa gacggcggcc tgcgcgggcg
     1801 tgaggtcgaa ggtttcgggg acgaggaggt tttgcgattc gggagcgcgc ccgctttcga
     1861 ggaggcggta cttgtcgaag aggatgcgct cgtgcgcgag gtgctggtcg atgagcagca
     1921 gaccttcggc gtcggcggcg acgacgtagg tgtctgcgag ctggccgagc gggcggatgt
     1981 ccgccgagag cgattcggcg gaggcttcgc gcacgaggcc ggcggcggag ccgagcggcg
     2041 ggagcgcgcc gcccgccgcg cgcgcacttg tcgagggcga gggcgcgggg ccggtcgatg
     2101 aagtgtcgcg tgtcgaggat gacgtgccgg gcgcttgagg gccgcgcggc gtcgttgtgt
     2161 cttcagggat ggccgcctcc gtcggcatcg aggctttgtc gtcagccgtc gcggcgtctt
     2221 cgttcgaaga atcacgcgcg gcagggcgtt cggcgtgacg cgcgtcgtcg cgccagagcg
     2281 aatcgtcggt cgtcgtcggc cattcgaatt cgatggactc ctgccgcgcc tcttccctcc
     2341 cggcgcgtga agtgatgtgg ctttcgctgc cgaagctcgc cgtgtcggtt cgacggaagg
     2401 cgtcggcgga ttctgttgac gaagcggggc gcgcgtcgtg tgcgtcgtgc gcgtgcggcg
     2461 agtcgtgaag gttcgcgtcg tcgcgcgtgg cgcggtgcgg cgactcgtcg cgcgcgtcgg
     2521 ggtcttcggt cgtgcggagg tatccgccgg cgacgagcgc gtcgcggacg gcgtcgcgga
     2581 cggcgtcggc aatcgcagag gtgcggcgga agcgcacctc cgtcttcgcg gggtggacgt
     2641 tcacgtccac ctcttcgggc ggcacgtcga ggaagaggag cgccgccggg aaggcgccct
     2701 gcgggagcac ggagcggaag ccttcgctga gcgcgcgcgt catcacgcgg tcgcggacgt
     2761 agcgaccgtt gacgaagagg aactgcgcgt cgcgcgtcgt gcgccgctcg cgcggggccg
     2821 agacgtagcc gcgtacgcgc gccacgcccg cgtgcgcgcc cttcacctcc aacaggctct
     2881 ccacgaactc cgcgccgaaa atctggtagg cgcgctcgcg caggtcggcg gcgggcgcgg
     2941 cgcgcagaat ttcccgcccg ttgctcgtca gggtgaaggc gatgtcgggg cgggcgagcg
     3001 cgtagtgcgt gacgaggttc gtgaggtggt agttctcggt cgcctcggag cggaggaatt
     3061 tgcggcgggc cgggacgttg aagaagaggt cgcgcgcggc gacggtcgtg ccgcgcgggc
     3121 gggccgcggg cgccacgtcg cgcatcctcc cgccctcgat gaggacgcgc gtgccttcgg
     3181 cctcgccttc gcgctgcgtg accagctcga cgcgcgcgac cgaggcgatg gacgcgagcg
     3241 cctcgccgcg aaagcccaac gtgccgatgc ggtcgaggtc ttcggtcgtg ttgatcttcg
     3301 aagtggcgtg gcgctcgaag gcgagcaccg cgtcgtcgcg cgtcatcccc tcgccgtcgt
     3361 ccgcgacgcg cagcaggcgg cgcccgcccc cctccgaatc gatctcgacg cgccgcgcgc
     3421 ccgcgtcaat cgcgttctct accagctcct tcgccacgga ggccgggcgc tccaccacct
     3481 cgcccgcggc gatctggttg gcgacgtgat cggagaggac gcggattttg gacatgacga
     3541 aagccgtgaa ccgtgaaccg taaatcgtga tgagtgaaaa gccgtgagcc gtgaaccgac
     3601 aaccgtaacg agagagaatg gattttaact tgtcacggtt tacggttcac ggttcacggc
     3661 cttcagaggt aaacaacttg gtggtcggtg gtgccgatgt tgatggcgtc gtgaatccat
     3721 tcgacgacgt aggcgccgtg gcgcgcgagc agggaggtga cgttgaggtg gcgctcctgc
     3781 aaagccttct cggggtagag ggcggtggcg gcgcgttcga gctggcggaa gagtgcgcgg
     3841 tcgcgcgcca tctgcgagcg gtggaagcgc gagcgcaaac catgtagctg atattcgacc
     3901 ttgcggcggc ccgtcttgag cgcgtcgccg agcgtggggt cgaaatgggt gagccgctcg
     3961 cgcaaagttt cgagcgcgcg gcctatggcc tcttcggtct cgtcgaaggc gcgggcggcg
     4021 tcggcgccga ggtgttcttc gacgacgcgc gcggtgacgg cgtcgaggcc ggcgaagaag
     4081 tcttcgatgc cgaggccgta gcgttcgagc gtgcggcccg tgcggcgctc gacgaccgtg
     4141 aggctcgcgc ggtgaaggat gggcgtcgcc ggtctttcaa gtagacgata gacttcgttg
     4201 gtctgcgcga agtaggcgat ctccgccgcg ccgccgtagt aggcgatggt cgggagcatg
     4261 aagtcctgca cgaccgagcg caaagtgacg ttggggctga agcgctccgg ctcgcgcgcc
     4321 gcgaggtcgg cgagttcgtc gagcgtccac tcttccgccc cgcccttcgc cgcgtagcgc
     4381 ccttccgcgt tgcgcgcgag cgcgtggcgc gcgccccgct cgtcgtggat
//
LOCUS       DBNGOH010000316         3636 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4812909, whole genome shotgun sequence.
ACCESSION   DBNGOH010000316 DBNGOH010000000
VERSION     DBNGOH010000316.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3636)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3636)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3636
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4812909"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2480
                     /locus_tag="AC159P_06080"
     CDS             <1..2480
                     /locus_tag="AC159P_06080"
                     /inference="COORDINATES: protein motif:HMM:NF012862.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HIR3695058.1"
                     /translation="AVHYTQTTEEMEARSKKDVGEAAKATQEALKRAAKNPADLLKVY
                     NQQLAGALGGAGTANTIRVWDVTTGREVQAVAGPAYVRRLHFSDDGRRLVVVGTQGDI
                     TSWDVGSGARLQAYGGSPAGPLGGMMPGGGLTTGDMSAMTAMINSAMSAAATGGTGGK
                     AVSVALSADGRTLAAGGSQTQSNFDASTLMSLGMRSGKMKREEYEKEIARTMQNVKVV
                     STGQVLLYDAATGQQTGVLASHGGGVEQVAFSRDGSLLATAATDNTIKVWDVAARREV
                     RALAGHAANINSLAFSPDRRLLASAGDDGATLLWDLQTGELLATLVSLFDGGDWLVVT
                     PDGLFDGSPRAWNQILWRYNGDTFEVAPIEWFFNEFYHPGLLSDIFNGKRPRAAADIS
                     KKDRRQPAVALTIEGARAQPPSSVTTRDVRVRIEVTDAKPDAENPQGSGARDVRLFRN
                     GSLVRVWRGDVLKGQPSVVLETSVPVVAGENRLVAYAFNRDNVKSKDAPLALMGAESL
                     KRAGTLYVLAVGVNVYANPQFNLKYAVADAREFGAEVERQQASLGRFRRTEVIPLLDG
                     DATKANVLDALRRLSGASGPPTLKAGLAQTLERLQPAQPEDAVVIFFAGHGTADGQRF
                     YLIPHDLGYDGSRTSIDDAGLRRMLAGSISDEELETAIEGLDAGQMLLVIDACNSGQA
                     LEAEEKRRGPMNSKGLAQLAYEKGMYILTAAESYQAALEAAQLGHGYLTYALVEEGLK
                     KGNADAEPKDGTLFAREWLDYATLRVPLMQEEKMKQSRGLGVQIAFVEGEEKSPDPDK
                     RSVQRPRVFYRRELETNPLVVAKPAAK"
     gene            complement(2636..3310)
                     /locus_tag="AC159P_06085"
     CDS             complement(2636..3310)
                     /locus_tag="AC159P_06085"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013702.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HIR3695059.1"
                     /translation="MTEAADSLGGREKGPARDRAMPQWVIDVIRPCVRVVSRALWHVE
                     LKGTGNIPAAGGLVIAANHQTYVDPFWITIPVRRPVRYLAWSEAFKNPILGRPMEWLG
                     AWPLVVERGNPTAYRRSIQWLRGGGVVMIFPEGSRAYADGEFGKFKTGAVRLALEAGA
                     PVLPVTLRGGNHVWPRGQRWPHTGRVEITYHPVHRLTMLPGEDARHCAKRETEALAEI
                     IRNSRQ"
     gene            complement(3307..>3636)
                     /locus_tag="AC159P_06090"
     CDS             complement(3307..>3636)
                     /locus_tag="AC159P_06090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695060.1"
                     /translation="VLLVEIERRCGDAACNARVFVGLTKEEARAYTGFVCERCERWTD
                     DELSERDIPDWWEELKVTSLDGVRPARAVGEGEPGEVVERMSEAWRRVGGPAQRGAGE
                     GEDEETF"
BASE COUNT          614 a         1301 c         1215 g          506 t
ORIGIN      
        1 tcgccgtcca ctacacgcag acgaccgagg agatggaggc gcgctcgaag aaggatgtcg
       61 gcgaggccgc gaaggcgaca caggaggcgc tgaagcgcgc ggcgaagaac cccgccgacc
      121 tgttgaaggt ctataaccag cagctcgcgg gcgcgctcgg cggggccggc acagcgaaca
      181 cgatcagggt ctgggacgtg acgacggggc gcgaggtgca ggcggtcgca ggccccgcgt
      241 acgtccggcg gctccacttc agcgacgacg ggcgcaggct cgtcgtggtc ggcacgcagg
      301 gcgacatcac ctcctgggac gtgggcagcg gcgcgcggct tcaggcttac ggcgggtcgc
      361 ccgcggggcc gctcggcggc atgatgccgg gcggcgggtt gacgaccggc gacatgagcg
      421 cgatgaccgc catgattaac agcgccatga gcgcggcggc gacgggcggc accgggggca
      481 aggcggtctc cgtcgcgctc agcgccgacg gtcggacgct cgccgcgggc ggctcgcaga
      541 cgcagagcaa cttcgacgcc tcgacgctca tgagcctcgg gatgaggtcg ggcaagatga
      601 agcgcgagga gtacgagaaa gagatcgccc ggacgatgca gaacgtgaag gtcgtctcga
      661 ccgggcaggt cttgctctac gacgccgcga cggggcagca gaccggcgtg ctcgcgagcc
      721 acggtggggg cgtcgagcag gtcgcgttca gcagggacgg ctcgctgctc gccacggcgg
      781 cgaccgacaa cacgatcaag gtctgggacg tggcggcgcg gcgcgaggtg cgcgcgctcg
      841 cgggccacgc ggcgaacatc aactcgctgg ccttctcgcc ggacaggcgg ctgctcgcct
      901 cggcgggtga cgacggcgcg acgctgctct gggacttgca gacaggcgag ctgctcgcca
      961 cgctcgtctc gctcttcgac ggcggcgact ggctcgtcgt cacgcccgac gggttgttcg
     1021 acggctcgcc ccgcgcgtgg aaccaaatcc tctggcgcta caacggcgac accttcgagg
     1081 tcgcgcccat cgagtggttc ttcaacgagt tctaccaccc cggcctgctc tccgacatct
     1141 tcaacggcaa gcgcccgcgc gccgccgccg acatctcgaa gaaagaccgc cgccagcccg
     1201 ccgtggccct gacaatcgaa ggagcacggg cacagccgcc ttcatccgtc acgacgcgcg
     1261 acgtgagagt caggatcgag gtgacggacg ccaaacccga cgcggagaac ccgcagggca
     1321 gcggcgcgcg cgacgtgcgg ctgttccgca acggctcgct cgtccgcgtc tggcgcggcg
     1381 acgtgctcaa gggccagccc tcggtcgtgc tcgaaacgag cgtccccgtc gtcgcgggcg
     1441 agaaccgtct ggtcgcctac gccttcaacc gcgacaacgt gaagagcaag gacgcgccgc
     1501 tcgcgttgat gggcgccgag agcttgaagc gcgcgggcac gctgtacgtg ctcgccgtcg
     1561 gcgtcaacgt gtacgcgaac ccgcagttca acctcaagta cgccgtggcc gatgcgcgcg
     1621 agttcggcgc cgaagtcgaa cgccagcagg cgagcctcgg tcgcttccgc cggacggagg
     1681 tcatcccgct cctcgacggc gacgcgacca aagccaacgt cctcgacgcg ctccgccgcc
     1741 tctccggggc gagcggcccg cccacgctca aagcgggcct cgcgcagacg ctcgaacgcc
     1801 tccagcccgc gcaacccgaa gacgccgtcg tcatcttctt cgccggccac ggcaccgccg
     1861 acggccagcg cttctacctc atcccgcacg acctcggcta cgacggcagc cgcacctcca
     1921 tcgacgacgc gggactgcgg cggatgctcg ccggcagcat ctccgacgaa gaactcgaaa
     1981 ccgccatcga aggactcgac gccggccaga tgctcctcgt catcgacgcc tgcaactcgg
     2041 gtcaggcgct cgaagccgag gagaagcgcc gcggcccgat gaactcgaaa ggtctggcgc
     2101 agctcgccta cgagaagggg atgtacatcc tcaccgccgc ggagagctat caggccgcgc
     2161 tcgaagccgc gcagctcggc cacggttatc tcacctacgc gctcgtcgag gagggcttga
     2221 agaagggcaa cgccgacgcc gagccgaaag acggcacgct cttcgcgcgc gagtggctcg
     2281 actacgcgac gttgcgcgtc ccgctcatgc aggaagagaa gatgaaacag tcccgcggcc
     2341 tcggcgtcca gatcgccttc gtcgagggcg aagaaaaaag tcccgacccc gacaagcgca
     2401 gcgtccaacg cccacgcgtc ttctaccgcc gcgaactgga aaccaacccg ctggtcgtgg
     2461 ccaaaccggc ggcgaaatag cgggcagcgg gcagcgggca gtaaaagcaa gaggcttgag
     2521 acgtaaagtc ccaagcctct tgctttgttg cttgaagagc gaagaggctt cggactttac
     2581 ctctcaacaa tcctcgctct tttgtcttga ctgcctactg cctgctgccc gctgcctact
     2641 gcctactgtt cctgatgatt tccgcgagcg cttcggtttc gcgtttggcg cagtggcgcg
     2701 cgtcttcgcc ggggagcatc gtgagtcggt ggacggggtg gtaggtgatc tcgacgcggc
     2761 ccgtgtgcgg ccagcgctgg ccgcgcggcc agacgtggtt gccgccgcgc agcgtgaccg
     2821 gcaggacggg cgcgcccgct tcgagcgcga ggcgcacggc gcccgtcttg aatttgccga
     2881 actcgccgtc ggcataggcg cggctgcctt cggggaagat catcacgacg ccgccgccgc
     2941 gcagccactg aatcgagcgc cggtaggccg tggggttgcc gcgctcgacg acgagcggcc
     3001 acgcgccgag ccactccatc ggccttccca ggatcgggtt cttgaaagcc tcgctccacg
     3061 cgaggtaacg gacggggcgg cgcacgggga tcgtgatcca gaacggatcg acgtaagtct
     3121 ggtggttcgc cgcgatgacg agtccgcccg cggcggggat gttccccgtc cccttcagct
     3181 cgacgtgcca cagcgcgcgc gagacgacgc ggacgcacgg gcgaatcacg tcgatcaccc
     3241 actgcggcat cgcgcggtcg cgcgccggcc ccttctcgcg cccgccgagc gaatccgccg
     3301 cttcggtcaa aacgtctcct cgtcttcgcc ttcgcccgcg ccgcgctgcg ccggcccgcc
     3361 gacgcgccgc cacgcctcgc tcatccgctc cacgacctcg cccggctcgc cctcgcccac
     3421 cgcgcgcgcg ggccgcacgc cgtcgagcga agtcaccttc aactcctccc accagtcggg
     3481 gatgtcccgc tcgctcaact cgtcgtccgt ccagcgctcg caacgctcgc acacgaaacc
     3541 cgtgtaggcg cgcgcctcct ccttcgtcag cccgacgaac acgcgcgcgt tgcacgccgc
     3601 gtcgccgcac cgccgctcga tttcgaccaa caacac
//
LOCUS       DBNGOH010000317         2166 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_964833, whole genome shotgun sequence.
ACCESSION   DBNGOH010000317 DBNGOH010000000
VERSION     DBNGOH010000317.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2166)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2166)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2166
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_964833"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..550)
                     /gene="galT"
                     /locus_tag="AC159P_06095"
     CDS             complement(<1..550)
                     /gene="galT"
                     /locus_tag="AC159P_06095"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007914524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HIR3695061.1"
                     /translation="MAWEERWHPLREEWVIVAAHRQDRPWSGGTVEHAEEELPEYLPD
                     CYLCPGNVRVSGKRNDDYTGTFVFDNDHPSVSPDAPEDLTPPTGIYHNRPARGLARVV
                     CYSPKHNLTLGELEPEEIANLLRVWQDEYVDLGSRPDIAHVLTFENKGEVVGVSNPHP
                     HGQIYATNFVFKTIETEVSASRR"
     gene            946..2091
                     /locus_tag="AC159P_06100"
     CDS             946..2091
                     /locus_tag="AC159P_06100"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015812285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HIR3695062.1"
                     /translation="MPGIKFALTLLFGVALVGGGTNMAEAKPGIRKQPFGKTADGRAV
                     DLYTLTNARGAEAGIITYGGIVVTLKVPDRKGVMGDVVLGCDTVDAYEKQTAYLGALI
                     GRYGNRIGKGEFTLDGTKYTLAKNNGENHLHGGLKGFDKVVWAATGSNPRGGPALKLT
                     YVSKDGEEGYPGNLTVTVVYTLTNNNELKIDYSATTDKATVLNLTHHSYFNLAGEGAG
                     DILGHQLYINADRFTPTDEGAIPTGELRAVRGTPMDFTKPTAVGALIDQERDEQIRFG
                     KGYDHNWVLNKRGAGALTLAARVYEPKTGRVMEVYTTEPGMQFYTGNYLDGTIDGKGD
                     KTYQRRHGFCLETQHYPDAPNKPGFPTTTLKPGRRYTQTTTYKFSAR"
BASE COUNT          425 a          718 c          692 g          331 t
ORIGIN      
        1 aacggcggct tgcgctcacc tcggtctcga tcgtcttgaa cacgaagttg gtggcgtaga
       61 tctgcccgtg cggatgaggg ttcgacacac cgaccacttc gcccttgttc tcgaaggtga
      121 ggacgtgcgc gatgtcggga cgcgagccga ggtctacata ttcgtcctgc cagacgcgga
      181 ggaggttcgc gatctcttcc ggctcaagct cgccgagcgt gaggttgtgc ttcgggctgt
      241 agcagacgac gcgcgccagc cctcgggccg ggcggttgtg gtagatgccg gtgggcggcg
      301 tcaaatcttc aggcgcgtcg ggcgagaccg aagggtggtc gttgtcgaag acgaaggtgc
      361 ccgtgtagtc gtcgttgcgc ttgccgctga cgcgcacgtt gccggggcag aggtagcagt
      421 ccggcaggta ctcgggcaac tcttcctcgg cgtgctccac cgtcccgccg ctccacggtc
      481 gatcctgacg gtgcgccgcc acgatcaccc actcttcgcg caaagggtgc cagcgctctt
      541 cccatgccat aacctgtctg tggctcctct cgccgcacac gaggacacgc gggggccgga
      601 ttattctcgg ttcagccggg ccgtgcgccg cgggcgcgcg actgctctcg tgcaagaata
      661 aagcctgaaa aaaacggttg ttgacgagcg atttaacgtt tactgtgata agccgaaaac
      721 gcccgtctgt caaggttatt ctgcgacacg ccgtgggcgc cgcgcggcgc cgttcgccgg
      781 tgttgacaac cgcaggcgcg gggcgttatc actcaggcga aggcgacgtt atcaatccga
      841 taagttggcg catgaacgag gcggcgggcc gcgacgccgt cggcccgcgc gcccgtgtgc
      901 tatgcgccgc ggggcgctca gtctaaaatg aaggagactc ttctcatgcc tgggatcaaa
      961 ttcgcgctga ccctgctgtt cggtgtcgcg ctggtgggcg gtggcacgaa catggccgag
     1021 gccaagccgg ggatcaggaa acagcccttc ggcaagacgg ctgacgggcg ggccgtggac
     1081 ctctacacgc tgacgaacgc gcgcggcgcc gaggccggca tcatcaccta cggcggcatc
     1141 gtcgtcacgc tcaaggtgcc cgaccgcaaa ggcgtgatgg gcgacgtggt cttgggctgc
     1201 gacaccgtgg acgcttacga gaaacagacc gcctacctcg gcgcgctcat cggtcgctac
     1261 ggcaaccgca tcggcaaggg cgagttcacg ctcgacggca cgaagtacac gctcgccaag
     1321 aacaacggcg agaaccacct gcacggcggc ctcaagggct tcgacaaagt cgtctgggcg
     1381 gcgaccggct cgaacccgcg gggagggccg gccctgaagc tgacctacgt cagcaaggac
     1441 ggcgaagagg gctaccccgg caacctgacg gtcacggtcg tctacaccct gaccaacaac
     1501 aacgagctga agatcgacta ctccgcgacc acggacaagg ccaccgtcct gaacctcacg
     1561 caccactcct acttcaacct cgcgggcgaa ggcgcgggcg acatcctcgg ccaccagctc
     1621 tacatcaacg ccgaccgctt cacccccacc gacgagggcg ccatccccac gggcgagctg
     1681 cgcgccgtcc gcggcacccc gatggacttc accaaaccga ccgccgtcgg cgcgctcatc
     1741 gatcaggaac gggacgagca gatacgcttc ggcaagggct acgaccacaa ctgggtgctg
     1801 aacaaacgcg gcgccggggc gctgacgctc gccgcccgcg tctacgagcc gaagaccggg
     1861 cgcgtgatgg aggtctacac caccgagccg ggcatgcagt tctacaccgg caactacctc
     1921 gacggcacca tcgacggcaa gggcgacaag acctaccagc gccgccacgg cttctgcctc
     1981 gaaacgcagc actaccccga cgcgcccaac aaacccggct tccccacgac cacgctcaaa
     2041 ccgggccggc gctacacgca gaccacgacg tataaattct ccgcccggta acaacggggg
     2101 aagggcaaaa gggtagaagg tcaggaggcg aagagggtgg atgagaggca cgctgcttct
     2161 catcca
//
LOCUS       DBNGOH010000318         3226 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_48028, whole genome shotgun sequence.
ACCESSION   DBNGOH010000318 DBNGOH010000000
VERSION     DBNGOH010000318.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3226)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3226)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3226
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_48028"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..719)
                     /locus_tag="AC159P_06105"
     CDS             complement(<1..719)
                     /locus_tag="AC159P_06105"
                     /inference="COORDINATES: protein motif:HMM:NF023418.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TgpA N-terminal domain-containing protein"
                     /protein_id="HIR3695063.1"
                     /translation="MSFERFFRASSYGMVACGVLALAVAGGVGLWELLVFAALLALSW
                     RLAGTRWQLSERKALAVILLSLPLFYLDWTLNRGAALAPGETHPSVAALAHFTLLLSL
                     IKLFQTKTDRDWIFLYLISFFQVLLAAGMTASLSFFLSLALYMFAAVMTVVCFELRKT
                     ARVAPAGESRLLVANDAKFSWRRGKKRRGGGERTLRRLPFAALGLLVLIFTLAAPVFL
                     ITPRSGSSALSMAAGKASSGYV"
     gene            complement(849..1694)
                     /locus_tag="AC159P_06110"
     CDS             complement(849..1694)
                     /locus_tag="AC159P_06110"
                     /EC_number="2.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016603 - glutaminyl-peptide
                     cyclotransferase activity [Evidence IEA];
                     GO_process: GO:0017186 - peptidyl-pyroglutamic acid
                     biosynthetic process, using glutaminyl-peptide
                     cyclotransferase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminyl-peptide cyclotransferase"
                     /protein_id="HIR3695064.1"
                     /translation="MTTIFALVLLFVGVGCQPQSASNGGSPPATSTAAARPSATPVAT
                     PEAPTLYTFEVVNSWPHDKKAFTQGLIFHDGNLLESTGQYGESTLRRVELKSGKVKKK
                     IDVGKQFFAEGITLLKGKLYQLTWQEHKAFVYDAASFNREKEFYLDGDGWGLTHDGES
                     LILSDGSDQLRFIDPETFKVKRTIKVADRGTPIRELNELEYVKGEIFANIWHDDRIAR
                     IDPRDGRVVGWIDLKGLLPANLITDGEAVLNGIAYDEAGDRLFVTGKLWPKLFEIRVV
                     KKESK"
     gene            complement(1817..2755)
                     /locus_tag="AC159P_06115"
     CDS             complement(1817..2755)
                     /locus_tag="AC159P_06115"
                     /inference="COORDINATES: protein motif:HMM:NF016289.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HIR3695065.1"
                     /translation="MTRWRFRIFAAPVVFALAACALAGCARREVAPPTKPPRFDEERA
                     HRRLVELCAMGPRNHGGEGKSRAERWIRESLVAAGAEVSVHEFRHTPKGAGVGALSFR
                     NIVGRIKPAEPRRVLVGTHYDTRSYAERDEDAARRGEPITGANDGGSGVAVLLEMAAL
                     WKDEPPPVGVDLIFFDGEDFGEGEDWDEYFLGSKAWAREHPDYRPEWGVILDMVGDAS
                     LRIRKERDSQARAPEVVKRLWAAAERVGSDAFADETGGRLLDDHTALLDRGVPVALVI
                     DFQYRWFHTTGDTVDKCSAASLGQVGRAVMEAVESP"
     gene            complement(2736..>3226)
                     /locus_tag="AC159P_06120"
     CDS             complement(2736..>3226)
                     /locus_tag="AC159P_06120"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HIR3695066.1"
                     /translation="LKYDLVVLDAFTIGGQIPFHLTTQEFMREVKNILAPGGVFLANI
                     NGSTRGRRSRILRSEYKTLATVFESLYVFPRLTDSERKQGVREPHPEGRRNIILVALN
                     GPGRWTRESVVSEAERLELAGAVRTPTFVEDARQFTDEPPPVADVPVLTDDYAPVDTM
                     AF"
BASE COUNT          556 a         1115 c         1038 g          517 t
ORIGIN      
        1 acgtagccgc tcgacgcctt gcccgcggcc atcgacaggg cgctgctgcc cgagcgcggc
       61 gtgatgagga agaccggcgc ggccagcgtg aagatcagca cgagcaggcc gagcgccgcg
      121 aacggcagac gacgcaacgt gcgctcgccc ccgccccgcc gcttcttccc ccggcgccac
      181 gaaaacttcg cgtcgttggc gacgagcaga cggctctcgc ccgcgggggc aacgcgcgcc
      241 gtcttccgca gctcgaaaca gaccaccgtc atcaccgccg cgaacatgta gagcgcgagc
      301 gacaggaaga aggagaggct ggccgtcatc cccgcggcga gcagcacctg gaagaatgag
      361 atgaggtaca ggaaaatcca gtcgcggtcg gtcttcgtct ggaagagctt gatgagcgag
      421 agcagcagcg tgaagtgcgc gagggcggcg acgctcgggt gcgtctcgcc cggcgcgagg
      481 gccgcgcccc tgttgagcgt ccagtcgagg tagaacagcg gcagcgacag caggatgacg
      541 gccagcgcct tgcgctccga aagctgccaa cgcgtccccg ccagccgcca cgacagcgcg
      601 agcagcgccg cgaagaccag caactcccac agccccaccc cgcccgccac ggccagcgcc
      661 agcacgccgc acgcgaccat gccgtaagac gaagccctga agaatctctc gaaactcacc
      721 acgttaaaac ggaaagcaaa tggtgaatgg taaatggtga atggtagttg aaggctattc
      781 gactgggacg atgaagcaca agtcgaacgg caatcaacac aatccgttta ccatttacta
      841 ttcaccattc acttgctctc ttttttcacc acgcgtatct cgaacagttt gggccagagc
      901 ttgccggtga cgaagaggcg gtcgccggcc tcgtcgtagg cgatgccgtt caggacggcc
      961 tcgccgtcgg tgattagatt ggcggggagc agacccttga ggtcgatcca accgacgacc
     1021 ctgccgtcgc gcgggtcgat gcgcgcgatg cggtcgtcgt gccagatgtt ggcgaagatt
     1081 tcgcccttca cgtattcgag ttcgttcagc tcgcggatcg gcgtgccgcg gtcggcgact
     1141 ttgatcgtcc gcttcacctt gaaggtctcg ggatcgatga agcggagctg gtccgagccg
     1201 tcgctcagga tcaggctctc gccgtcgtgc gtcagccccc agccgtcgcc gtccaggtaa
     1261 aactccttct cgcggttgaa gctcgccgcg tcgtacacga acgccttgtg ctcctgccac
     1321 gtcaactggt agagcttgcc cttgagcagc gtgatgccct cggcgaagaa ctgcttgccc
     1381 acgtcgattt ttttcttcac cttccccgac ttcagctcga cccggcggag cgtggactcg
     1441 ccgtactgcc ccgtgctctc cagcaggttg ccgtcgtgga agatcaaccc ctgcgtgaaa
     1501 gccttcttgt cgtggggcca cgagttgacc acctcgaagg tgtagagcgt cggcgcttcg
     1561 ggggtggcga ccggcgtcgc ggaggggcgg gccgcggcgg tcgaggtcgc gggcggcgag
     1621 ccgccgttgc tcgcggactg aggctgacaa ccgacgccga cgaacagaag gacgagggcg
     1681 aatatcgtcg tcataagatt cacaggcttc gctttgcgca tgtcggagtt catctttaac
     1741 aggccaacct tcgtcgagga taacgcgacc cggcgtccgc atcgaagtag agcgcgcggg
     1801 ccacaggccc cgcgcctcag ggagactcta ccgcctccat caccgccctg ccgacctgcc
     1861 ccaggctcgc cgccgaacac ttgtccacgg tgtcgccggt cgtgtggaac cagcgatact
     1921 gaaagtctat gacgagcgcc acgggcacgc cgcggtcgag cagcgccgtg tggtcgtcca
     1981 gaagccgccc gcccgtctcg tccgcgaagg cgtcggagcc gacgcgctca gccgccgccc
     2041 acagacgctt caccacttcg ggcgcgcgcg cctgcgagtc gcgctccttc ctgatgcgga
     2101 gcgacgcgtc gccgaccatg tcgaggatga cgccccactc cggcctgtag tctgggtgct
     2161 cgcgcgccca cgccttcgac ccgaggaaat attcgtccca gtcctcgccc tcgccgaaat
     2221 cctcgccgtc gaaaaagatc aaatccaccc cgacgggcgg cggctcgtcc ttccagaggg
     2281 cggccatctc aagcagcacc gcgacgccgg agccgccgtc gttcgcgccc gtgatcggct
     2341 cgccgcgccg cgccgcgtcc tcgtcgcgct cggcgtacga gcgcgtgtcg taatgcgtcc
     2401 cgaccagcac ccgccgcggc tccgcgggtt tgatgcgccc gacgatgttt cggaagctca
     2461 gagcgccgac gcccgccccc ttcggcgtgt gccggaactc gtgcacgctg acctcggcgc
     2521 ccgccgcgac gagcgactcc ctgatccagc gctccgcgcg cgacttgccc tcgccgccgt
     2581 ggttgcgcgg ccccatcgcg cacagctcga ccagacggcg gtgtgccctc tcttcgtcga
     2641 agcgcggcgg cttcgtcggg ggcgcaacct cgcgcctcgc gcaacccgcg agcgcgcacg
     2701 cggcgagtgc gaagacgacg ggcgcggcga atattctaaa acgccatcgt gtcaaccggc
     2761 gcatagtcgt ccgtgagcac gggcacgtcc gcgacgggcg gcggctcgtc cgtgaactgt
     2821 cgcgcgtcct cgacgaaggt cggggtgcgg accgcgccgg ccagctccaa acgctcggcc
     2881 tcgctcacca cggactcacg cgtccagcgg cccggcccgt tcaaggcgac gaggatgatg
     2941 ttgcgccgcc cctcggggtg cggctcgcgc acgccctgct tgcgctccga gtcggtcagg
     3001 cgcgggaaga cgtagaggct ctcgaagacc gtggcgagag tcttgtactc cgagcgcagg
     3061 atgcggctgc ggcgcccgcg cgtcgagccg ttgatgttcg ccagaaagac gccgcccggc
     3121 gcgagaatgt ttttcacctc ccgcatgaat tcctgcgtgg tcaggtggaa agggatctgc
     3181 ccgccgatgg tgaacgcgtc cagcacgacg aggtcgtact tgagcg
//
LOCUS       DBNGOH010000319         5895 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2431605, whole genome shotgun sequence.
ACCESSION   DBNGOH010000319 DBNGOH010000000
VERSION     DBNGOH010000319.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5895)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5895)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5895
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2431605"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(234..3911)
                     /gene="mfd"
                     /locus_tag="AC159P_06125"
     CDS             complement(234..3911)
                     /gene="mfd"
                     /locus_tag="AC159P_06125"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HIR3695067.1"
                     /translation="MSSPVESVPVLQGVLRRVTGSAEFGRLAAEVAGGARVVSVSGLT
                     SAPARALAVAALQRETGRRFAVVVEANRDTEGWERDLAFWCGALRGACDEARGGPVLV
                     LPSSESDPYAGASPHAETLERRALALWRLARGGSPFVLLTSRALVRRTVRPAKLLEAG
                     AVLRRDEEFAPEHLVELLHASGYVREDPVGAVGEFSLRGGILDVWSPGSETPVRVEFF
                     GDTVDSIRRFDPETQLSVAQLQEAEVVPMREVSVGAEDFRLWAMLARERWPDEARARA
                     LKDRTVYAEEGETFAGWEWMIPLVAGTDASAFDYLGDAVLVVDEPAGVEQYLATVYET
                     LAARYAENEAAGEIGLKPEELYLTPEELRGKLSAQRRVELRALGRTAAMIDERFAGEA
                     EQPAARIGRERAAPQPLFLFPAVEEASEVEWRSLPARRYHGRVPDLAADVRRAREETR
                     AATLFVVPSAGVAERVGEILSEYNVAARVALAGEGGAAAEDFAAIVTVGRLSGGFELP
                     GAGLIVHVESDLFDEAGDGTVERRAPGTTTTTSGRKRKSKTAAFLSDFRDLKVGDYVV
                     HIDHGIARFGGLQTLELGGRKGEFMLLFYAEDAKLYVPVERLDLVQRYSSAEGHTPAI
                     DRLGGLGWHKTKAKAKRAMRDMADELLRLYAERKLVQGYAFSGDSPWQREFEEAFEYV
                     PTPDQETTIEDVKRDMEQTVPMDRLLCGDVGYGKTEVAMRAAFKSVMDGKQVAVLAPT
                     TVLAYQHFETFRARFAAFPVQIQLLSRFRSAREQKEIVKRVEVGEIDIIIGTHRILSK
                     DIAFRDLGLVVVDEEQRFGVAHKERLKQLKKRVDVLTLSATPIPRTLNMSLMGLRDMS
                     IIETPPRDRLAIQTQVVQFSESVIKSAIELEIGRGGQVFFIHNRVETIDTVAALVQRL
                     VPRARVTVAHGQMNEKEMEQVMLDFVAYKADVLVATTIIENGIDIPRANTIIINRADH
                     YGLSQLYQLRGRVGRSNRRAYAYLLIPSEAELTPIARRRLAAIREFSDLGAGFRVAAL
                     DLELRGAGNLLGGEQSGHMDALGFDLYTQMLERTVAELRGEDVEDETSVSINLGVDVA
                     IPEDYVSDMGQRLRTYKRIASARDDEQLDQIRRETEDRYGRLPESVERLFEYARLRRT
                     AEEVGAVSIDRTPTGLAVKLRETARVSPDKLLALVAKGEGASFSPSGVLRIEGGAAGG
                     GAGTELVEAARRALLEIRAEG"
     gene            complement(3983..4474)
                     /gene="rfaE2"
                     /locus_tag="AC159P_06130"
     CDS             complement(3983..4474)
                     /gene="rfaE2"
                     /locus_tag="AC159P_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014098924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HIR3695068.1"
                     /translation="MSEKIIEGGDRLGRVRAGLREGGRRLVFTNGCFDILHVGHVRYL
                     QRARLLGDALLVAINSDRSVRALKGEGRPVMSEAERAEMLAALACVDFVTVFEEDSPR
                     RLIAELLPDVLVKGGDYALDEIHGREEVEAAGGRVLALPFVEGASTTNIIERIAKTVN
                     REP"
     gene            4622..>5895
                     /locus_tag="AC159P_06135"
     CDS             4622..>5895
                     /locus_tag="AC159P_06135"
                     /inference="COORDINATES: protein motif:HMM:NF012379.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HIR3695069.1"
                     /translation="MSTPFIEENDQTRARREHLERIRELVGNAYPNKFRRSAVAPGAE
                     GEDTITSVARDFKSREPQTEEGARPAPEQLEEANAELNGITVRVSGRIATPPRVMGKA
                     AFVHLSDGTERLQIYLRKQDAKAISNDTGQPIDDFDSGWQLLQYLDHGDFVGVEGYLF
                     VTKTGELSVHVNTIEFLSKALVPMPDKLHGISDPEIRQRQRYADLIAGSLKVEHYPDQ
                     LSTREVFERRSKLITELRRHLDAHGYVEVETPMLSPIASGAAARPFKTHHNALDIDLF
                     LRIAPELYLKRLLVGGFERVYELNRNFRNEGIGYRWNPEFTMLEFYCAYMDADGMMDF
                     AEAMLGATIEKATNRTQAHFDNEVIEFGRPFERLTMKESVSKYWSKVFKSPSASKFQK
                     TWLDDPERTRFLEHFLLKLAPESIFHVSAEIH"
BASE COUNT         1034 a         2204 c         1700 g          957 t
ORIGIN      
        1 ggcccacgcg agcgcgagcg ccagcgcggc gaggccgagt ttgagacgac gggccgaagc
       61 gattttgaac atcacgatat tttcactcct ctaaaactgt cggggcggct gctcaagcct
      121 tgttcgaatg ttattgatta ggccgccgcc gaacccgcgg tcgggcggcg cgcgcgggat
      181 gcaacatggg tggccggaag cgaaactttc atgcccggac gcgaacgcgc attctaccct
      241 tcggcgcgga tttcgagcaa cgcgcggcgg gccgcctcga ccagctcggt gcccgcgccg
      301 ccgccggcgg cgcctccctc gatgcgcaag acgccgctcg ggctgaaact ggcgccctcg
      361 cctttggcga ccagcgccag cagcttgtcg ggcgagacgc gcgccgtctc cctcagcttg
      421 acggcgaggc ccgtcggcgt gcggtcgatc gagacggcgc cgacctcttc cgccgtgcgc
      481 ctgaggcgcg cgtactcgaa gagtctttcg accgactcgg gcagccgccc gtagcggtct
      541 tcggtctcgc gcctgatctg gtcaagctgt tcgtcgtcgc gcgccgaggc gatgcgcttg
      601 tacgtcctca gccgctgccc catgtccgag acgtagtcct cggggatggc gacatcgacg
      661 ccgaggttga tcgacacgct cgtctcgtcc tccacgtcct cgccgcgcag ctccgccacc
      721 gtccgctcaa gcatctgcgt gtaaagatcg aagccgaggg cgtccatgtg cccagactgt
      781 tcgccgccca aaaggttccc cgcgccgcgc agctccaaat ccagcgccgc cacgcggaag
      841 cccgcgccca agtccgaaaa ctccctgatg gccgccagcc gccgccgcgc aatcggcgtc
      901 agctcggctt ccgaagggat gagtaaatag gcgtatgcgc ggcggttaga gcgcccgacg
      961 cggccccgca gttgataaag ctgcgacagc ccgtagtggt cggcccggtt tatgatgatt
     1021 gtgttcgcgc gcgggatgtc tatgccgttc tcgataatcg tcgtcgccac cagcacgtcg
     1081 gccttgtagg cgacgaaatc cagcatcacc tgctccatct ccttctcgtt catctgcccg
     1141 tgcgcgaccg tcacgcgcgc gcgcggcacg agcctttgga cgagcgccgc cacggtgtcg
     1201 atcgtctcca cgcggttgtg gatgaagaag acctgcccgc cgcgcccgat ttccagctcg
     1261 atggcggatt tgatgaccga ctcggagaac tgcacgacct gcgtctgaat cgccagacgg
     1321 tcgcgcggcg gcgtctcgat gatcgacata tcgcgcagcc ccatcaagct catgttgagc
     1381 gtgcgcggga tcggcgtcgc cgacagagtg agcacgtcca cgcgcttctt caactgcttc
     1441 agacgctctt tatgtgcgac gccgaagcgc tgctcttcat ccacgacgac gaggccgagg
     1501 tcgcggaagg cgatgtcctt cgagagtatc cggtgcgtgc cgatgatgat gtcgatctcg
     1561 cccacctcga cgcgcttgac gatctccttc tgctctctgg ctgagcgaaa gcgcgagagc
     1621 aactgaatct gcacggggaa cgccgcgaag cgcgcgcgga aagtctcgaa gtgctggtac
     1681 gcgagcacgg tcgtcggcgc gagcaccgcg acctgcttgc cgtccatcac ggacttgaac
     1741 gcggcgcgca tggcgacttc cgtcttgccg tagcccacgt cgccgcagag cagcctgtcc
     1801 atcggcacgg tctgctccat gtcgcgcttc acgtcctcga tggtcgtctc ctggtcgggc
     1861 gtcggcacgt actcgaacgc ctcctcgaac tcgcgctgcc acgggctgtc gcccgagaac
     1921 gcgtagccct gcacgagctt ccgttcggcg tacaagcgca gcagctcgtc ggccatgtcg
     1981 cgcatcgcgc gcttcgcctt ggccttcgtc ttgtgccacc cgagaccgcc cagacggtcg
     2041 attgccggcg tgtgcccttc ggcgctcgaa tagcgctgga cgaggtcgag ccgttcgacc
     2101 ggcacgtaga gcttcgcgtc ctccgcgtag aagaggagca tgaactcgcc cttgcgcccg
     2161 ccgagttcga gcgtctgcaa gccgccgaag cgcgcgatgc cgtggtcgat gtggacgacg
     2221 taatcgccga ccttcaaatc gcggaagtcc gacaggaacg ccgccgtctt cgacttgcgt
     2281 ttcctgccgc tcgtcgtggt cgtcgtcccc ggcgcgcgcc gctcgaccgt cccgtcgccc
     2341 gcctcgtcga acaggtcgct ctcgacgtgg acgatcaacc cggcgcccgg cagctcgaac
     2401 ccgcccgaca gcctgcccac cgtgacgatg gccgcgaagt cctccgccgc cgcgccgccc
     2461 tcgcccgcca gcgccacgcg cgccgcgacg ttgtactccg agaggatttc gccgacgcgc
     2521 tcggccacgc ccgcgctcgg caccacgaag agcgtcgcgg cccgcgtctc ttcgcgtgcg
     2581 cgccgcacgt ccgccgcgag gtcgggcacg cgcccgtggt aacgccgcgc gggcagactc
     2641 cgccactcga cctccgaagc ctcttcgacc gccgggaaca gaaacagagg ctgcggcgcc
     2701 gcacgctcgc gcccgatacg cgccgcgggc tgctcggcct cgcccgcgaa ccgctcgtcg
     2761 atcatcgcgg cggtgcgccc caacgcgcgc agctcgacgc gccgctgcgc ggacaacttc
     2821 ccacgcagct cttccggcgt caaataaagc tcttccggct tgaggccgat ctcgcccgcc
     2881 gcctcgttct cggcgtaacg cgcggcgagc gtttcgtaga cggtcgccag atactgctcg
     2941 acgcccgccg gctcgtccac gacgagcacc gcgtcgccga ggtagtcgaa cgcgctggcg
     3001 tccgttcccg cgacgagcgg aatcatccac tcccaccccg cgaacgtctc gccctcctcg
     3061 gcgtagaccg tccgatcctt cagcgcgcgc gcacgcgcct cgtccggcca acgctcgcgc
     3121 gccagcatcg cccacagacg gaagtcctcc gcgccgaccg acacctcgcg catcggcacg
     3181 acctccgcct cctgcaactg cgcgaccgag agctgcgtct ccgggtcgaa cctcctgatc
     3241 gagtccaccg tgtcgccgaa gaactccacg cgcacgggcg tctcgctgcc cggcgaccac
     3301 acgtcgagga tgcccccgcg caaactgaac tcgcccacgg cgcccaccgg gtcttcgcgc
     3361 acgtagcccg acgcgtgcaa caactccacc agatgctccg gcgcgaactc ctcgtcgcgc
     3421 cgcaagaccg cccccgcttc gagcagcttc gcgggcctca ccgtcctgcg caccagcgcc
     3481 cgcgaagtca gcagcacgaa cggcgagccg ccgcgcgcca aacgccacag cgccagcgcg
     3541 cgccgctcca acgtctccgc gtgcggcgac gcgcccgcgt acgggtcgct ctccgacgaa
     3601 ggcagcacga gcaccggccc gccccgcgcc tcgtcgcacg cgccccggag cgccccgcac
     3661 cagaacgcga ggtcgcgctc ccacccttcg gtgtcgcggt tcgcctcgac caccaccgcg
     3721 aaccttcgcc ccgtctcgcg ctgcaacgcc gccaccgcca gcgcacgcgc cggggccgaa
     3781 gtcagcccgc tcaccgacac cacgcgcgcg cccccggcga cctccgccgc cagacgcccg
     3841 aactccgcgc tccccgtcac gcgccgcagc acgccctgaa gcaccggcac cgattcgacc
     3901 ggactactca taactttcaa aagccgtgaa ccgtaaaccg taaaccgtga tgagaaaaga
     3961 cctgttttcc tcttatcacg gtttacggtt cacggtttac ggtctttgca attctttcga
     4021 tgatgttcgt cgtcgaagcg ccctcgacga aggggagcgc gaggacgcgc ccgcccgccg
     4081 cctcgacctc ctcgcgcccg tgtatctcgt cgagcgcgta gtcgccgccc ttcacgagca
     4141 cgtcgggcag caactccgcg atcaggcgcc gcggcgagtc ctcttcgaac accgtcacga
     4201 agtccacgca cgcgagcgcc gccagcatct ccgcgcgctc ggcctcgctc atcacgggtc
     4261 gcccctcgcc cttcagtgcg cgcaccgagc ggtcgctgtt gatggcgacc agcagcgcgt
     4321 cgccgaggag gcgcgcccgc tgcaagtagc gcacgtgccc gacgtgcagg atgtcgaaac
     4381 acccgttcgt gaacaccagc cgccgccccc cttcgcgcaa ccccgcgcgc acgcggccca
     4441 gcctgtcgcc gccctctatg atcttctcgc tcattggtta gtcaatggta aatgaaaagc
     4501 cgtgaaccgg caaaagccgt gaaccgtgaa ccgtgaaccg tgacaagata aaaactgctt
     4561 tctacttgtc acggtttacg atttacggtt tacggctctt atcgtttacc attgaccgct
     4621 tatgagcacg cccttcatcg aagagaacga ccagacgcgc gcacggcgcg agcacctcga
     4681 acgcatccgc gaactcgtcg gcaacgccta ccccaacaag ttccgccgct ccgcggtcgc
     4741 cccgggcgca gagggcgagg acacgatcac ctccgtcgcc cgcgacttca aaagccgcga
     4801 gccgcaaacg gaagaaggcg cgcgccccgc gcccgaacag ctcgaagagg cgaacgccga
     4861 attgaacgga atcaccgtgc gcgtctcggg ccgcatcgcc acgccgccgc gcgtgatggg
     4921 caaggccgcc ttcgtccacc tctccgacgg cacggagcgc ctgcaaatct acctccgcaa
     4981 gcaggacgcc aaagccatca gcaacgacac cggccagccc atcgacgact tcgacagcgg
     5041 ctggcagctt cttcaatacc tcgaccacgg cgacttcgtc ggcgtcgaag gctacctctt
     5101 cgtcaccaag accggcgagc tatccgtcca cgtcaacacc atcgagttcc tctcgaaggc
     5161 gctcgtcccg atgcccgaca agctccacgg catctccgac cccgaaatcc gccagcgcca
     5221 gcgctacgca gacctcatcg ccgggagcct caaggtcgaa cactacccgg accagctttc
     5281 cacccgcgaa gtcttcgagc gccgctccaa gctcatcaca gagctgcgcc gccacctcga
     5341 cgcgcacggc tacgtcgaag tcgagacgcc catgctctcg cccatcgcct ccggcgccgc
     5401 cgcccgcccc ttcaagacgc accacaacgc gctcgacata gacttattcc tccgcatcgc
     5461 gcccgaactc tacctcaagc gcctcctcgt cggcggcttc gagcgcgtct acgaactcaa
     5521 ccgcaacttc cgcaacgaag gcatcggcta ccgctggaac cccgaattca ccatgctcga
     5581 attctactgc gcctacatgg acgcagacgg catgatggac ttcgctgagg cgatgctagg
     5641 agcgacgatt gagaaagcga caaacaggac acaggctcat tttgacaatg aagtcatcga
     5701 gttcggtcgc ccttttgagc gacttacaat gaaggagtct gtttccaaat actggagcaa
     5761 agtctttaag tcaccaagcg ccagtaagtt tcaaaaaacg tggttggatg atccagagcg
     5821 tacccgattc ttagaacact ttcttcttaa gctagctcct gaatcgatct tccatgtctc
     5881 agcggagata cataa
//
LOCUS       DBNGOH010000320         3161 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1882045, whole genome shotgun sequence.
ACCESSION   DBNGOH010000320 DBNGOH010000000
VERSION     DBNGOH010000320.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3161)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3161)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3161
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1882045"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..612
                     /locus_tag="AC159P_06140"
     CDS             <1..612
                     /locus_tag="AC159P_06140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695070.1"
                     /translation="LADNLSALDQYFMGFRGHHAAFVVALLVFVGSSVALGVFQPKDD
                     IRKRIGADCFSTDTAFGYRPAELYAMLDRYRSEDYKAHKLFIFLDLIYPLLYSVSLAI
                     MLGYLIPLLAPEEHTRIHYLTLLPLAAAVFDVLENLSMLLILNLREGNAAGRSDALAC
                     FSSAMTMTKLVLVYATLLLTVVGILLTLLAVFTRRKRPAAHAA"
     gene            808..1515
                     /locus_tag="AC159P_06145"
     CDS             808..1515
                     /locus_tag="AC159P_06145"
                     /inference="COORDINATES: protein motif:HMM:NF013250.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HIR3695071.1"
                     /translation="MLREEFAGANKTGTSVLSPLERRMARRVVPHIPRWLETYHLTML
                     TLVWCALIVASGYLAAARDIRWLWGVSLMIAFQYVTDHFDGKVGKYRNTGLVKWGFYM
                     DHLLDYVFLCSVLAAYGLLVPDRARLHLFAVLAVFGGFMVNSFLAFAATAKFKISVLR
                     LGPTEFRLALVAINTLVILYGPKYLAKALPYVAAGGLLCLCLVVYRTQKEIWRRDMEL
                     KKAVNGEPSTVTRKNRF"
     gene            1637..1936
                     /locus_tag="AC159P_06150"
     CDS             1637..1936
                     /locus_tag="AC159P_06150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695072.1"
                     /translation="MAENENAAAAAEAPATDAGTGTESSGDAAEATETAAAETTAAEP
                     QAEAAHEEITYHAVEKDGQVTAIWEMQGRKHLRTIDPRSRQGQEILRLYTAEHGD"
     gene            complement(2006..2944)
                     /locus_tag="AC159P_06155"
     CDS             complement(2006..2944)
                     /locus_tag="AC159P_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011630757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HIR3695073.1"
                     /translation="MEKIVFLERNTLDADVRAPGFEHEWHDYGETRPEEIWERLRDAT
                     IAVVNKSPLGADVLARLPRLKLVAVAATGTDNIDLAFCRERGITVTNVRGYAVRTVPE
                     HVLALVLALRRNLFAYREDVRRGRWARAGQFCLLTHPIRDLAASTLGVVGYGALGQAV
                     AALMRAVGMRVLVAERRGAATAREGRTPFAEVLRESDVLTLHVPLNAETRGLVGREEL
                     ATMRPSALLINCARGGVVDEGALAEALRAGVIAGAGVDVLTREPPDGDDNPLLSTDLP
                     NLILSHHVAWASREAMQALADQLIDNIEAFVNSREP"
     gene            complement(2956..>3161)
                     /locus_tag="AC159P_06160"
     CDS             complement(2956..>3161)
                     /locus_tag="AC159P_06160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695074.1"
                     /translation="SGASSRRSSSPSEKNINHEDTKGRKKDEEDKETGQRLLLFTFLS
                     LCLLFVSLCVFVVKFFPFNLKGR"
BASE COUNT          554 a         1087 c          976 g          544 t
ORIGIN      
        1 ctcgcagata acctctccgc gctagaccaa tacttcatgg gcttcagagg ccatcacgcg
       61 gccttcgtcg tcgcgctcct cgtcttcgtc ggctcgtccg tcgcgctcgg cgtcttccag
      121 ccgaaggacg acatccgcaa gcgcatcggc gccgactgct tcagcaccga cacggccttc
      181 ggctaccgcc ccgccgaact ctacgcgatg ctcgaccgct accggagcga agactacaag
      241 gcgcacaagc ttttcatctt cctcgacctc atctacccgc tgctctattc cgtctctttg
      301 gcgatcatgc tcggctacct catcccgctc ctcgcgcccg aagagcacac gcgcatccac
      361 tacctcacgc tcctgcccct cgccgccgcc gtcttcgacg tgctcgaaaa cctctcgatg
      421 ctcctcatcc tcaacctgcg cgagggcaac gccgccgggc gctccgacgc gctcgcctgc
      481 ttttcgagcg cgatgacgat gaccaagctc gtcctcgtct acgcgacgct gctgctcacc
      541 gtcgtcggca tcctcctgac cctgctcgcc gtcttcacgc gcaggaagcg gcccgcggcc
      601 cacgccgcct gacgcccttt tgtcacgcca cctgtcgaat ttttccggcg gcgccccgcc
      661 gtcgcgcctt ccccgcgaac cctcctccca agccgcgcgg gccggaaaaa cgcgggccgc
      721 cgccgcttcc gcgcgcgcgc cggggcgtgg ctccaagttt gctccatcac taatcctctc
      781 ccgacgttga gggggcggaa gacgaagatg ctgagagaag agttcgcagg agcaaacaag
      841 accggcacgt ccgtgctgtc gcctctggag agacggatgg cgcggcgcgt cgtgccgcac
      901 atcccgcggt ggctggagac gtaccacctg acgatgctga cgctcgtctg gtgcgcgctc
      961 atcgtcgcgt ccggctatct ggccgcggcg cgcgacatcc gctggctgtg gggcgtgtcg
     1021 ctgatgattg ccttccagta cgtgaccgac cacttcgacg gcaaggtcgg caagtaccgc
     1081 aacacggggc tggtcaagtg gggcttctac atggatcacc tgctcgacta cgttttcttg
     1141 tgctcggtgc tcgccgccta cggcctgctc gtccccgacc gcgcgcgcct gcacctgttc
     1201 gcggtgctgg ccgtcttcgg cggcttcatg gtcaactcgt tcctcgcgtt tgccgccacc
     1261 gcgaagttca agatcagcgt gctccgcctc ggcccgaccg agttccgcct cgcgctcgtc
     1321 gccatcaaca cgctcgtcat cctctacggc ccgaagtacc tcgccaaagc gctcccgtac
     1381 gtcgccgcgg gcggcctcct ctgcctctgc ctcgtcgtct accgcaccca gaaggaaatc
     1441 tggcgccgcg acatggaact caaaaaggcc gtgaacggtg aaccgtcaac cgtgacaaga
     1501 aaaaaccggt tctgacttgt cacggttgat ggttcaccgt tcacggcttt gttgctagac
     1561 acgcccgccg cgcgtgtgcc aaaattcctg ccgtaaataa tcacaaaaat caaagacaat
     1621 tcggagagga atgtacatgg cagagaacga gaacgccgcg gcggcggcgg aagcgcccgc
     1681 gaccgacgca gggacgggga cggagtcgag cggagacgcc gcggaggcga ctgagaccgc
     1741 cgccgctgag acgaccgccg ccgagccgca ggccgaggcc gcgcacgaag agatcaccta
     1801 ccacgccgtc gagaaggacg ggcaggtcac ggccatctgg gagatgcagg gccgcaagca
     1861 cctgcgcacc atcgacccgc gctcgcgcca gggccaggaa atcctgcgcc tctacacggc
     1921 ggaacacgga gactgaaaag ccgtgaaccg tgaaccgtca accgtgacaa gaggaaatca
     1981 gttttcactt gtcacggttg acggttcacg gttcacggct attaacgaac gcctcgatgt
     2041 tgtcgatgag ttggtcggcg agggcctgca tggcttcgcg gctggcccag gcgacgtggt
     2101 gcgagaggat caggttcggt aggtctgtcg agaggagcgg gttgtcgtcg ccgtcgggcg
     2161 gctcccgcgt caacacgtcc acgcccgcgc cggcgatgac gccggcgcgc aaggcttccg
     2221 cgagcgcgcc ttcgtccacc accccgccgc gcgcgcagtt aatcaacaga gcagagggcc
     2281 gcatcgtggc gagttcttcg cgcccgacga ggccgcgcgt ctctgcgttg agcgggacgt
     2341 ggagcgtcaa gacatcgctc tcgcgcagca cctcggcgaa gggcgtgcgg ccctcgcgcg
     2401 cggtggccgc gcccctgcgc tcggcgacga ggacgcgcat cccgacggcg cgcatgagcg
     2461 cggcgaccgc ctgcccgagc gcgccgtagc cgacgacgcc gagcgtgctc gcggcgaggt
     2521 cgcggatcgg gtgcgtcagc aggcagaact gaccggccct cgcccaacgc cctcgccgca
     2581 cgtcctcgcg gtaggcgaag aggttgcggc ggagcgcgag cacgagcgcg aggacgtgtt
     2641 cgggcacggt gcgcaccgcg tagccgcgca cgttcgtgac ggtgatcccg cgctcgcggc
     2701 agaaagcgag gtcgatgttg tctgtgccgg tggcggcgac ggcgacgagc ttcagccgcg
     2761 gcagacgggc gagcacgtcg gcgccgagcg gcgacttgtt gacgacggcg atggtcgcat
     2821 cgcgcagccg ctcccagatc tcttcggggc gcgtctcgcc gtagtcgtgc cactcgtgct
     2881 cgaagccggg cgcgcgcacg tcggcgtcga gcgtgttgcg ttcgaggaag acgatttttt
     2941 ccataagtta tcacttcacc tgccctttaa gttgaacgga aaaaatttaa ccacaaagac
     3001 acataaggac acgaagagaa ggcaaagaga aagaaaggta aagagaagca aacgctgccc
     3061 ggtctctttg tcttcttcgt ccttcttcct gccctttgtg tcttcgtggt taatgttttt
     3121 ctcagagggc gaagaactgc gtcgagacga agccccagag g
//
LOCUS       DBNGOH010000321         2943 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1514937, whole genome shotgun sequence.
ACCESSION   DBNGOH010000321 DBNGOH010000000
VERSION     DBNGOH010000321.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2943)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2943)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2943
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1514937"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(706..1203)
                     /locus_tag="AC159P_06165"
     CDS             complement(706..1203)
                     /locus_tag="AC159P_06165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695075.1"
                     /translation="MNNNFKVSLESPQSGFMSLRLRAGAESFVAVMSHAPYDSMRELI
                     EGLTALVAGGQGFTVKWNAEPEEYDFEFEPAGAEVELRVARYAGHRRAGDAPAVVFLH
                     RGPRAEVCQSFWREFKRLRDRRETDVYEQNWRRAFPERELHDFTRALKHSRQRAADSR
                     QQEEV"
     gene            1413..2699
                     /locus_tag="AC159P_06170"
     CDS             1413..2699
                     /locus_tag="AC159P_06170"
                     /inference="COORDINATES: protein motif:HMM:NF017760.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3695076.1"
                     /translation="MSEASSERGDVGYVELVRRNPDFRRLWLGQVVSQLGDWFDTIAV
                     YTLVLKLTGSGRALGLVLVARFLPSVVVGPLAGVLADRFSRRAIMIWSDLLRALVVLG
                     FLFVRRPEHLWLLYALTVLQLALSAFFEPARAAAVPSVVSERELLPANALSSVTWSAM
                     LTIGAAIGGEMTGRFGTDAAFVLDSLTYLASAALIYGVRLPKREPRPKTRLTLSKALG
                     VADTIEGIRYVRTRPRVLALLLVKFAWGFGGGILLLLPVFGETIWAVAGRAGSGTGIL
                     FAARGIGTAIGPIAARRLAAGSRRAMQRFIGVGFLMGGAFYIAFGLANNFALALFVLA
                     VAHMGGSILWVFSTVLLQSTVEDQFRGRVFAAEFTLMTLAMAASNYATGEALDRFSLS
                     PRTVAVALGVVFLLPGLLWLLTERLWNRDAPSGRPD"
BASE COUNT          445 a         1068 c          899 g          531 t
ORIGIN      
        1 cagcgcgacg accgccgccc agtaggcgcg cgccgcgcgg ttgtaatcgg acatgtctag
       61 gagcgccacc acgactcccg attgcggatt gtggattgcg gattgcggat tgaaaaagag
      121 agaacgccta acgccgttcg tcgctctatg actgcgaacc cgcaacccgc gatccgcaat
      181 cggtaatgcc taaaacggtt ccgccgccgg cagcttgggc cgggagcaaa tcggccttcg
      241 ccgccgacga cggagggggc aagaggcggg catcacgttg tggcactcgc ccgccaattg
      301 gggtgagaaa cagttcgtca ccttttcgac agaggcgcga aggagcacgc gccgggtgac
      361 caaacttttg cggctttacg aaccccgtgc ggtgggttcg tgaggccccc gaaggcgccc
      421 ctgcaagccg cgccgtattc attaacaacc ggcgtgccgc aaccgcgtgc cgcccgccga
      481 cgcgccaaaa gccgcgaaac gactccattt tccgaacgcg acgccgcgcc cgccgagcgg
      541 cccacggcgg gcgacggtca gcgtgacggg tcactatgat tgggcgttca gaatgaagga
      601 gcaggcagca ggcagcaggc agcgggcagt taagacagaa cgacgaagag gctcgggact
      661 tcacctcccg agcctcttcc gtcttcggac tccagactgc ggactctaga cttcttcctg
      721 ctgcctgctg tctgctgccc gctgcctgct gtgtttcagc gcccgcgtga agtcgtgcag
      781 ctccctttcg gggaaggcgc ggcgccagtt ctgctcgtac acgtccgtct cgcgccggtc
      841 gcgcaggcgt ttgaactcgc gccagaagga ctggcagacc tctgcgcgcg ggccgcggtg
      901 caagaagacg accgcgggcg catcgcccgc gcggcggtgg cccgcgtagc gcgcgacgcg
      961 cagctcgacc tccgcgcccg ccggctcgaa ctcgaagtcg tactcctccg gctcggcgtt
     1021 ccacttcacg gtgaacccct ggccgcccgc gacgagcgcc gtcagcccct cgatcaactc
     1081 gcgcatcgag tcgtaaggcg cgtgcgacat gacggcgacg aagctctccg cgcccgcccg
     1141 caggcgcagc gacataaacc cgctctgcgg gctttcgaga ctgactttga agttgttgtt
     1201 cacgccgcga ttatactgaa gaagtaggcg gtaggcagta ggcagtgaaa gcaagttgag
     1261 aattttggga ttcgaaattt tcaaatccga gctacgcgtt ttcttaactg cctactgcct
     1321 accgcctact tcttcgttgt ttgacaagcg cacgcgcgag cgcgcactct cttcgtcacg
     1381 tcttcatccg gacttctttc ggcggtcatg tcttgagcga ggcttcatcc gagcgcggcg
     1441 acgtgggcta cgtcgaactc gtgcgccgca accccgactt ccggcggctg tggctggggc
     1501 aggtcgtcag ccagctcggc gactggttcg ataccatcgc cgtctacacg ctcgtcctca
     1561 agctcaccgg ctcggggcgc gcgctcggcc tcgtgctggt cgcgcgcttt ctgccgagcg
     1621 tggtggtggg gccgctcgcg ggcgtgctcg ccgaccgctt cagccgccgc gcgatcatga
     1681 tctggagcga cctcctgcgc gcgctggtcg tgctcggctt cctcttcgtc cgccgccccg
     1741 aacacctctg gctgctctac gcgctgacgg tgctgcaact cgcgctgtcg gccttcttcg
     1801 agccggcgcg cgccgccgct gtcccttcgg tcgtctccga gcgcgagctg ctgccggcca
     1861 acgcgctctc gtccgtcacg tggtcggcga tgctcaccat cggcgcggcc atcggcggcg
     1921 agatgaccgg gcgcttcggc accgacgccg ccttcgtcct cgactccctg acctacctcg
     1981 cctccgccgc gctcatctac ggcgtccggc tcccgaaacg agagccgcga ccgaagacgc
     2041 ggctcacgct ctcgaaggcg ctcggcgtcg ccgacacgat cgagggcatc cgctacgtgc
     2101 ggacgcgccc gcgcgtgctc gcgctgctgc tcgtcaagtt cgcgtggggc ttcggcggcg
     2161 gcatcctcct gctgctcccc gtcttcggcg agacgatctg ggccgtggcg ggccgcgcgg
     2221 gcagcggcac cggcatcctc ttcgccgcgc gcggcatcgg cacggccatc ggccccatcg
     2281 ccgcgcgccg cctcgccgcc ggctcgcgcc gcgcgatgca gcgcttcatc ggcgtcggct
     2341 tcctcatggg cggcgccttc tacatcgcct tcggcctcgc gaacaacttc gcgctcgcgc
     2401 tcttcgtcct cgccgtcgcg cacatgggcg gcagcatcct ctgggtcttc tccaccgtcc
     2461 tcttgcagag caccgtcgaa gaccagttcc gcgggcgcgt cttcgccgcc gagttcaccc
     2521 tcatgacgct ggccatggcc gcctccaact acgccaccgg cgaagccctc gaccgcttca
     2581 gcctctcgcc ccgcaccgtc gccgtcgccc tcggcgtcgt cttcctcctc cccggcctcc
     2641 tctggctcct caccgagcgg ctgtggaaca gagacgcgcc gagcgggcgc cctgactgag
     2701 caagaccttc gtgttcacct ttcccttctt ttcctttatc atcttcggca caactcactc
     2761 gtcgtcgcat tccgaagttg ggtaagactg acggcgagcg ccgcgaggcc gggccgcacc
     2821 cgtacaattc gacgaagcag agaggtacat ccgacatgcg acgaagaagc atcgttctgt
     2881 tcagcaccgc tctcatcatc accctcgccc tgctcgccgc cgcgacgaac gcccagacaa
     2941 ggc
//
LOCUS       DBNGOH010000322         2711 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4447981, whole genome shotgun sequence.
ACCESSION   DBNGOH010000322 DBNGOH010000000
VERSION     DBNGOH010000322.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2711)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2711)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2711
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4447981"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            234..1037
                     /locus_tag="AC159P_06175"
                     /gene_synonym="epsI"
     CDS             234..1037
                     /locus_tag="AC159P_06175"
                     /gene_synonym="epsI"
                     /inference="COORDINATES: protein motif:HMM:NF023410.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006605 - protein targeting [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase C-terminal domain/associated protein
                     EpsI"
                     /protein_id="HIR3695077.1"
                     /translation="MKTHRNFWLLFVLLVACGALIHARERAGEVGVERRALKEFPEEL
                     GGWRRRGPDARFDAATEAVLRADDYISRDYATADNNKAASLYVGYYNTQRVGATYHSP
                     LNCLPGSGWELTEPATIKINPADGSPAFEANRYVIRYGSQSQLLVYWYQGRGRAVASE
                     YVDKMYTVLDSMRLRRSDGSMVRILVPVAGSEQAALDAAVSFAAEVEPASRLTCRTEA
                     APPPSPAGAFRRKCARARLCTKAAASPSSCPRTTKRATSAPPSRPFPVS"
     gene            944..1879
                     /locus_tag="AC159P_06180"
     CDS             944..1879
                     /locus_tag="AC159P_06180"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR3695078.1"
                     /translation="MYKGRRIAVVMPAHNEARHIGPAVKTLPRFVDHIIVVDDASRDA
                     TAEAARACGDARVEVIRAERNQGVGGATMLGYRRGLELASDVLVKMDGDGQMDPDYLA
                     PLLDAVVEHGYDYAKGNRFLAGASLAEMPKVRLFGNVLLTFLTKLASGYWHIFDPQNG
                     YTAVRAEALRRLDLGAVHQRFFFENDMLSQLNYHNLRVKDVAIPARYREEVSDLNPFK
                     IGLTFPPLLLRRFFRRLYQKYVLRDFSPIALFIAVGLLLFAWGFCFGLYTWVKSNLTG
                     HPAPTGTIMLALLPLILGFQLLLQAIVLDIQETPK"
     gene            2169..>2711
                     /locus_tag="AC159P_06185"
     CDS             2169..>2711
                     /locus_tag="AC159P_06185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695079.1"
                     /translation="MKSLIGAAGKSLLLCLVALTVLALGQREARADEVFIAGYTNGCF
                     NCISPPNTSATQTATLSGLTYVNSTFSGTTAGGFRGLGGNPIAPPVQNVNNLGAFSLA
                     TTPATYTGNTFSLRVTFTAPEGINGGGSPVLTATLTGTVTSDPNSGGVRLDFDNSPIL
                     ITFTDTNCGATTIPGQQTTCG"
BASE COUNT          456 a          964 c          840 g          451 t
ORIGIN      
        1 cgggcgccgc aagggcggtg acgagaagac gacgaagacg acaaccgccg acgccgccgg
       61 cgacgagacg ggcgtggtcg gccccagcgc gccctcggcg gcgatgtcct cggtcggcgc
      121 gacgaagaat acctgaacgc atcgcgggtg cgtcgaatcc tcgaacgcat cacgcgtgaa
      181 gtcacggaag aattcttgaa cggttcgccg aagaatcatc gaacggttga cggatgaaga
      241 cgcatagaaa tttctggttg ctgttcgtgc tgctcgtcgc gtgcggcgcg ctgattcacg
      301 cgcgcgagcg tgcgggcgag gtgggcgtcg agcggcgcgc gctgaaggag ttccccgaag
      361 agctgggcgg gtggcgcagg cgcggcccgg acgcgcgctt cgacgcggcg accgaggccg
      421 tgctgcgcgc agacgactac atctcgcgcg actacgcgac cgccgacaac aacaaagcgg
      481 cctcgctcta cgtcggctac tacaacacgc agcgcgtcgg cgcgacctac cacagcccgc
      541 tcaactgcct gcccggctcg ggctgggagc tgaccgagcc ggccaccatc aagatcaacc
      601 ccgccgacgg ctcgcccgcc ttcgaggcca accgctacgt catccgctac ggcagccaga
      661 gccagctcct cgtctactgg tatcagggcc gcgggcgcgc cgtggcgagc gagtacgtgg
      721 acaagatgta caccgtcttg gacagcatgc gcctgcgccg ctcggacggc tcgatggtgc
      781 gcatcctcgt gccggtcgca ggctccgagc aggcggccct cgacgccgcc gtctcgttcg
      841 ccgccgaagt cgagcccgcc tctcgcctta cgtgccggac tgaagcagcg ccgccgccgt
      901 cgcccgcggg cgcgttccgc cgaaagtgcg cccgcgcccg cctatgtaca aaggccgccg
      961 catcgccgtc gtcatgcccg cgcacaacga agcgcgccac atcggccccg ccgtcaagac
     1021 ccttccccgt ttcgtagacc acatcatcgt cgtcgatgac gcgagccggg acgcgaccgc
     1081 cgaagcggcg cgcgcgtgcg gcgacgcgcg cgtcgaggtg atccgcgccg agcgcaatca
     1141 gggcgtcggc ggcgcgacca tgctcggcta ccgcaggggg ttggagctgg cgtccgacgt
     1201 gctcgtgaag atggacggcg acgggcagat ggacccggac tacctcgcgc cgctgctcga
     1261 cgcggtggtc gagcacggct acgactacgc caagggcaac cgcttcctcg ccggggcgtc
     1321 gctggccgag atgccgaagg tcaggctctt cggcaacgtc ctgctcacct ttctcacgaa
     1381 gctcgcctcc ggctactggc acatcttcga cccgcagaac ggctacaccg ccgtccgggc
     1441 cgaggcgctg cgccgtctcg acctcggcgc cgtccaccag cgcttcttct tcgagaacga
     1501 catgctcagc cagttgaact accacaacct gcgcgtgaag gatgtggcga tcccggcgcg
     1561 ctacagggaa gaggtctccg acctcaaccc tttcaagatc gggctgacct tcccgcccct
     1621 gctgctgcgc aggttcttcc gccgcctcta ccagaaatac gtgctgcgcg acttctcgcc
     1681 catcgcgctc ttcatcgccg tcgggctgct cctcttcgcc tggggcttct gcttcggcct
     1741 ctacacctgg gtcaagagca acctcacagg ccaccccgcg ccgacgggca cgatcatgct
     1801 ggcgctcctg cccctgatac tcggcttcca gctcctcttg caggccatcg tgctcgacat
     1861 tcaggagacc ccgaagtgag cgggcggtca aggcatccac gaattcggtc ggtttgacac
     1921 gttgtagtag tgtgataatt tcgttaactt tttagtacgg tttcgcgcgg acactcatcc
     1981 cgcctcaacc tatactccgt caaacgcctt ctcccccgaa tcgagtgtca gtctgttagg
     2041 tcggcctgtg cggggcgctg cctgaggact tcccgcaaag cccgtcgtgt cagcggctat
     2101 tgctccagcc gccgccgcgc cgtcgcttcg aacgtccgta tactatgtgt aaccactggg
     2161 aggacagtat gaaaagttta atcggggcgg cgggcaagtc tttattgcta tgcctcgttg
     2221 ccttgacagt tctcgcgctc ggccagcgcg aggcccgtgc ggatgaggtc ttcatcgcag
     2281 ggtataccaa cggctgcttc aactgcatct cgccgccgaa cacctctgcg acgcagacgg
     2341 ccacgctctc cgggctgacc tacgtcaact cgaccttctc gggcaccacc gccggcggct
     2401 tccgcggcct cggcggcaac cccatcgcgc cgcccgtgca gaacgtcaac aacctcgggg
     2461 ccttctcgct cgcgaccacg cccgcgacct acacgggcaa caccttcagc ctgcgcgtga
     2521 ccttcacggc gcccgagggg attaacggcg gcggctcgcc ggtcttgacg gcgaccttga
     2581 cgggcaccgt gacgagcgac ccgaactcgg gcggcgtgcg tctggacttc gacaactcgc
     2641 cgatcctgat cactttcacg gacacgaact gcggggcgac gaccatcccg gggcagcaga
     2701 cgacctgcgg g
//
LOCUS       DBNGOH010000323         2528 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1698938, whole genome shotgun sequence.
ACCESSION   DBNGOH010000323 DBNGOH010000000
VERSION     DBNGOH010000323.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2528)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2528)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2528
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1698938"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1911
                     /locus_tag="AC159P_06190"
     CDS             <1..1911
                     /locus_tag="AC159P_06190"
                     /inference="COORDINATES: protein motif:HMM:NF015168.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HIR3695080.1"
                     /translation="MEHESFENESIARLMNENYVNIKVDREERPDLDQIYMTAVQMMT
                     RHGGWPLTVFLTPDLVPFYGGTYFPPEDRYNMPGFPRILAGVADAYRSKPEEVRQSAW
                     EILSELRRVGLGGESEKALSPELLDDAFESISRGYDPEWGGFGGAPKFPSPMNLEFLL
                     RYRLRSGNPQALEMATHTSRMMAKGGMYDQLGGGFHRYSTDRRWLVPHFEKMLYDNAL
                     LARTYLHLHQLAGKSFYRRVAEETLDYVLREMTDEGGGFYSTQDADSEGVEGKFFVWD
                     KAEIIDHLGAEDGALFCAYYGVSEEGNFEGHNILHVPKEATEVAREAGVPVERLTEAV
                     ERGRRVLFDVREKRIKPGRDEKVITSWNGLMLEAFAEAAAILERADYLEAAEHNARFI
                     LDNLRREDLLLHVYKDGAAKHVGFLDDYSFYASGLIALYETTGAAQWLTEAVRLTEKM
                     VAEFWDAEAGGFFYTGESGEKLIVRSKDYFDNATPSGNSVALGVLLRLAVLTGEEKYR
                     RMATTIFRLVGDSVSRFPSAFGYALSALDFYISTPREVVIVAPASAPHDRALAREVWK
                     RYLPNKVLVQAAEGDEQSLALLPLLLDRKAVGGRPTAYVCENYSCRQPVSTPEALAAQ
                     LDAEPSGDAAAV"
     gene            complement(1996..>2528)
                     /locus_tag="AC159P_06195"
     CDS             complement(1996..>2528)
                     /locus_tag="AC159P_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012493886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HRDC domain-containing protein"
                     /protein_id="HIR3695081.1"
                     /translation="RTYTDRHSLKELARELLGMDMSKQQQSSDWGADVLSEAQIAYAA
                     SDARITLNLYDRLRGLFEERGTWDAALRAALIKPTEPGQARPRRRATKAPDFQLTAEE
                     KRLVLALKKWRREHAHALRVPAYMICQDKTLEHLARERPATLEALSAIYGLGESKITR
                     FGAELLRALQEAGADE"
BASE COUNT          428 a          869 c          800 g          431 t
ORIGIN      
        1 atggagcacg agtctttcga gaacgaatcg atcgcgcggc tgatgaacga gaactacgtc
       61 aacatcaagg ttgaccgcga ggagcgcccc gacctcgacc agatttacat gaccgccgtg
      121 cagatgatga cgcgccacgg cggctggccg ctcacggtct tcctcacgcc cgacctcgtc
      181 cccttctacg gcggcaccta cttcccgccc gaagaccgtt acaacatgcc cggcttcccg
      241 cgcatcctcg ccggcgtcgc ggacgcttac cgcagtaagc ccgaagaggt caggcagtcc
      301 gcttgggaaa tcctctcgga gctgcgccgc gtcggcctcg gcggagagtc ggagaaggct
      361 ctgtcgcccg aactgctcga cgacgctttc gaatccatca gccgcggtta cgaccccgag
      421 tggggcggct tcgggggcgc gccgaaattc ccctcgccga tgaacctcga attcctgctg
      481 cgctaccgcc tgcgctccgg caacccgcag gcgctcgaga tggcgacgca cacctcgcgc
      541 atgatggcga agggcgggat gtacgaccag ctcggcggcg gcttccaccg ttattcgacc
      601 gaccgccggt ggctcgtccc gcacttcgag aagatgctct acgacaacgc gctgctcgcg
      661 cgcacctacc tgcacctcca ccagcttgcg ggcaagagtt tttaccggcg cgtcgcggag
      721 gagacgctcg actacgtctt gcgcgagatg acggacgagg gcggcggctt ctactcgacg
      781 caggacgccg actcggaagg cgtcgagggc aagttcttcg tgtgggacaa agccgagatc
      841 atcgaccacc tcggcgcgga agacggcgcg ctcttctgcg cctactacgg cgtcagcgaa
      901 gagggcaact tcgaggggca caacatcctt cacgtgccga aggaagcgac cgaggtggcg
      961 cgcgaggcgg gcgtgccggt cgagcgattg accgaggccg tcgaacgcgg gcggcgcgtg
     1021 ctcttcgacg tgcgcgagaa gcgcatcaag ccgggccgcg acgagaaggt catcacgtct
     1081 tggaacggcc tgatgctcga agccttcgcc gaggccgcag ctatcctcga acgcgccgac
     1141 tacctcgaag ccgccgagca caacgcgcgc ttcatcctcg acaacctgcg ccgggaagac
     1201 ctcctgctcc acgtctacaa ggacggcgcg gcgaagcacg tcgggttcct cgatgactac
     1261 tctttctacg cctcgggcct gatcgcgctc tacgagacga ccggcgccgc gcaatggctg
     1321 accgaggccg tgcggctgac agagaagatg gtcgcggaat tctgggacgc ggaggcgggc
     1381 ggctttttct acacgggcga gagcggcgag aagctgatcg tccgctcgaa ggactacttc
     1441 gacaacgcga cgccttccgg caactcggtc gcgctcggcg tcctgctgcg cctcgccgtc
     1501 ctgaccggcg aagagaaata caggcggatg gcgaccacca tcttccgcct cgtcggcgac
     1561 agcgtctcgc gcttcccctc ggccttcggc tacgcgctct cggccctcga cttctacatc
     1621 tcgacgccga gagaggtcgt catcgtcgcg cccgcctcgg cgccgcacga ccgcgccctc
     1681 gcgcgcgaag tctggaagcg ctacctgccc aacaaagtcc tcgtgcaggc cgcggagggc
     1741 gacgagcagt cgctcgcgct cctgccgttg ctgctcgaca ggaaagccgt gggggggcgg
     1801 cccacggcct acgtctgcga aaactatagc tgccgccaac cagtgtccac ccccgaagcg
     1861 ctcgccgcgc agctcgacgc cgaaccgtcc ggcgacgcgg cggcggtctg aggcggatca
     1921 ctttacctct catgtagaag ctgattgatg agtcctggac gggattcatc aatcagcttc
     1981 ttctatgtcc ttcgctcact cgtccgctcc cgcctcctgt agcgccctga gcagctcagc
     2041 gccgaagcgc gtgatcttcg attcgccgag gccgtagatg gccgacaggg cttcgagcgt
     2101 cgcggggcgt tcgcgcgcga ggtgttcgag cgtcttgtcc tgacagatca tgtaggccgg
     2161 gacgcgaagc gcgtgcgcgt gctcacgtct ccacttcttg agcgccagca cgagtcgctt
     2221 ctcctccgcg gtcagttgaa agtcgggcgc cttcgtcgcc cgccgccgcg gcctggcctg
     2281 gcccggctcg gtgggcttga tgagcgccgc gcgcaacgct gcgtcccacg tgccgcgctc
     2341 ttcgaacaga ccgcgcagcc tgtcgtagag gttcagggtg atgcgcgcgt cggacgccgc
     2401 gtaagcgatc tgcgcctcgc tcaacacgtc cgcaccccag tctgagcttt gctgctgctt
     2461 cgacatgtcc atgccgagca attcgcgggc gagttctttc aggctgtgac ggtccgtata
     2521 ggtgcgca
//
LOCUS       DBNGOH010000324         5948 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1699143, whole genome shotgun sequence.
ACCESSION   DBNGOH010000324 DBNGOH010000000
VERSION     DBNGOH010000324.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5948)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5948)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5948
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1699143"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1410
                     /locus_tag="AC159P_06200"
     CDS             <1..1410
                     /locus_tag="AC159P_06200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695082.1"
                     /translation="PPPLKRWIPSTESACERLHAPAQRLLAFAAALFHAPAHARTRAF
                     AAEVTAYFERHEAPQPESATPPTATRRAPHAHLRGDATREHDEGAHDSAETFAATDNA
                     ATTARFEHDASTHCTLSRADADGRHDAETATPTSPTRRSGGDARDEVSKDSGPLIDAR
                     AVGAEVAEELAPAPPWRDLEGGETALGGVLFLLNFFLHLRLPECFDEDFPLSEHVTGW
                     GLAELVARALLAEADCGDAYEEDPLWAVLARLDGRREGEPPAASLRVGEDYRAPAHWL
                     EHFAANDAQPWSFHVSGPRLVLLHAGLFPAAVQTLAGDAPARVAAGIVADYRARGVAF
                     EGVDISFEGLADEIAADENATTRASDVIAEVDRASVFASFGRVPLSAGLRRWMGWTLP
                     FMKYVLARALADGEEALTPCELARTLLLKRGRLYATATHVDLVMEMSQVSFEVRRAGF
                     DASPGWVRDLVRVVAFHFE"
     gene            1424..3559
                     /locus_tag="AC159P_06205"
     CDS             1424..3559
                     /locus_tag="AC159P_06205"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012234.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR3695083.1"
                     /translation="MTTPQQQQTNAFAGLAPNAETHFRLYYYAAVSRLLGYMQRLGGA
                     GGAAREGYQFLQGYEDELRAAMPGGGGEEEARAWWRAEIGAWEQETRARLPLRELAKT
                     LGLSHGELIALVLAGVVEEDIRFGSVFAALQEPLPARRPCLGLLGALAGGDEERGGGD
                     WWSAARRLMDAGLIVAENRAAPRSEWVMRVEPHVWDAVRGRDLGRISAECSLVSRREF
                     MPLKKLALDEDLRRRLAGIPAVVARGQLGAVVLRGMADSGRRAVMGALARATRRDLLF
                     YDRRGAPPSPADDLWHIVGPLASLTGALPVVAVDPAPGETIELPPLQGYSGAVGVVMR
                     REGGVGGPALERAVTLTLPAPAAEHRRRVWERRLGTQAGADLPDIVERFLLPLGNVER
                     AAGVAATYALLERRETVTTADVQLACRSLNRQKLDTLAQRLEVAGGWEDLVVNHVTAS
                     QLDELERRCRHRERLLSNLGAGFRNSLNCGVRALFNGPSGTGKTLAAKVLAAVLQSDV
                     YRVDLASIVNKYIGETEKNLSQLLARAEELDVILLIDEGDSLMTGRTDVKSANDRYAN
                     LETNYLLQRIETYQGIVVVTTNAGNRIDQAFKRRFDVLIDFQPPDPRERLLIWRHHLP
                     AAHRVSDAHLQRLAGHCALTGGQIRNAALHAALLAVVAGRDGEVEDADLEAAVSREYR
                     KAGASCPLGQAPVRNGHHAALTQFLAEIG"
     gene            3623..>5948
                     /locus_tag="AC159P_06210"
     CDS             3623..>5948
                     /locus_tag="AC159P_06210"
                     /inference="COORDINATES: protein motif:HMM:NF025078.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="eCIS core domain-containing protein"
                     /protein_id="HIR3695084.1"
                     /translation="MQIAQTKSRGRAGGETTDSAAPDSLAATGDATRMPAYALGQDAA
                     PTIPLLGGGLVQTKLAIGSAGDPYEGEADAVAAHVTADKEGASPPSISNVTARALAPS
                     APAVGGGGEAVQKESEPAPAADEEEQEEESALPVQRQPAAEEEQEPVQLETDEEEPDE
                     SVQRQPQEDEQEPVQLVQRQAAVEEEEPAVEQQEEEAEPVQTKAKPGRGPRDEEEEER
                     KERERRAEGAINNRQGGSPLDEETRSHVERRMGQDFSDVRVHDDAQSADANKALKSRA
                     FANRNHIFLGAGESRKDKRLLAHELTHIVQQGGVSRRPPRNGAGTAEPPPSAGPQPGG
                     VTKPAAGGQQPPSALPAAQTPALAGTAPAGTTPTAAKTPPPVTAAPSGKAQGATAPPA
                     ATAAPGAAQTGLTGVITPAKPADLAAPSGVAPAPAAGAEPKAAGAEVKPAEAQAGAAG
                     ATPETATGKPAGEAGATPEAEAGKAPAEGAAAEAAGEAQAAPAGGVPEELGGAAGGAM
                     AEGGAAPAVEGGKEAAAGGGGADVSAEGGEAEGGGSEEEGAAEMEERMAAAAGEKKSE
                     GEGAGAGEGGGTAEAAGEGSAAEEPEPEGEKTGEEPEQLAVPEPEQAAAAAGGEGGGG
                     EPPMPAEQSDTISEEAAADALSGGGGDMQEAGEAVKEANAAEGEEEEAAAPAAAGASE
                     EAEVGAAGGGAEAPVGEAAPAETPGAEAAAEGESEEAGAAPEAGAESGELAPAERDAA
                     MSSLAAGGGGEGGGGGGGGGGGAIPDKEEPEAPDV"
BASE COUNT          953 a         2131 c         2202 g          662 t
ORIGIN      
        1 cctccgccct tgaagcgctg gattccttcg accgagagcg cctgcgaacg tctgcacgcg
       61 cccgcgcaac gcctgctcgc cttcgccgcc gcgctcttcc acgcgcccgc gcacgcgcgc
      121 acgcgcgcct tcgccgcgga ggtcacggcc tacttcgaac gacacgaagc accgcaacct
      181 gagtccgcca caccgccgac cgccacacgc cgcgcgccgc acgcgcacct gcgcggcgac
      241 gcgacgcgcg aacacgacga aggcgcacac gactccgccg agacgttcgc ggcgaccgac
      301 aacgcggcga ccaccgctcg cttcgagcac gacgcttcga cgcactgcac gctctcgcgt
      361 gccgacgcgg acgggcggca cgacgcagag acggccacgc caacgtctcc gacgcgacgg
      421 tcgggcggcg acgcgcgcga cgaagtgtcg aaggacagcg gccctctcat cgacgcacgc
      481 gccgtgggtg cagaggtggc ggaagaactc gcgcccgcgc cgccgtggcg cgacctcgaa
      541 gggggcgaga cggcgctcgg cggcgtcctc ttcctgctca acttcttcct ccacctgcgg
      601 ctgcccgaat gcttcgacga ggacttcccg ttgtccgagc acgtcaccgg ctggggcctc
      661 gccgaactcg tcgcgcgcgc gctgctcgcg gaagcggact gcggcgacgc gtacgaagaa
      721 gaccctctgt gggccgtgct cgcgcggctc gacggcaggc gcgagggcga gcctcccgcg
      781 gcttccttac gcgtcggcga agactaccgc gcgcccgcgc actggctcga acacttcgcc
      841 gcgaacgacg ctcagccgtg gagcttccac gtctcaggcc cgcgccttgt gctccttcac
      901 gcgggcctct tccccgccgc cgtgcagacg ctcgcgggcg acgcgcccgc acgggtcgcc
      961 gccggaatcg tcgcggacta ccgcgcgcgc ggcgtcgcct ttgaaggagt cgatatctcc
     1021 ttcgagggac tcgctgatga gatagccgct gacgagaacg cgacgacgcg ggcgagtgac
     1081 gtgattgcag aagtagaccg cgcgagcgtc ttcgcctcct tcggtcgtgt gcctttgtcc
     1141 gccgggttgc ggcggtggat ggggtggact ttaccgttca tgaagtacgt gctggcgcgg
     1201 gcgctcgcgg acggggaaga ggcgctgacg ccgtgtgagc tggcgcgcac gctgctcctc
     1261 aagcgcgggc gtttgtatgc gacggcgacg cacgtcgatc tcgtcatgga gatgagtcag
     1321 gtctcgttcg aggtgcgccg cgcggggttc gacgcgtcgc cgggttgggt gcgcgacctc
     1381 gtgcgcgtgg tcgcgtttca tttcgagtga ggagggacga gcgatgacga cgccgcagca
     1441 gcaacagacg aacgccttcg cgggcctcgc gccgaacgcc gagacgcact tccgcctcta
     1501 ctactacgcg gcggtctcgc gcctgctcgg ctacatgcag cggctcggcg gggccggggg
     1561 cgcggcgcgg gagggttatc aattcttgca gggctacgag gacgagctgc gcgcggcgat
     1621 gccggggggc ggcggcgaag aggaggcgcg cgcgtggtgg cgcgcggaga tcggcgcgtg
     1681 ggaacaggag acgcgcgcgc gtctgccgct gcgcgagctg gcgaagacgc tcggcctctc
     1741 gcacggcgag ctgatcgcgc tcgtgctggc gggcgtggtc gaggaagaca tccgcttcgg
     1801 gtcggtgttc gccgcgttgc aggagcctct gcccgcgcgt cgcccgtgcc tcgggctgtt
     1861 gggggcgctg gcgggcggcg acgaggagcg cggcggcggc gactggtggt cggcggcgcg
     1921 ccgcctgatg gacgcggggc tgatcgtcgc ggagaaccgc gccgcgccgc gctcggagtg
     1981 ggtgatgcgc gtcgagccgc acgtgtggga cgcggtgcgc gggcgcgacc tcgggcgcat
     2041 cagcgccgag tgcagccttg tctcgcggcg cgagttcatg ccgctgaaaa aactggcgct
     2101 cgacgaagac ctgcgccgga ggctcgcggg catccccgcg gtcgtcgcgc gcgggcagct
     2161 cggcgccgtc gtgttgcgcg gcatggccga cagcgggcgg cgcgcggtga tgggtgcgct
     2221 ggcgcgcgcg acgcggcgcg atctgctgtt ctacgaccgg cgcggcgcgc cgccctcgcc
     2281 cgccgacgat ttgtggcaca tcgtcgggcc gctggcttct ttgacgggcg cgttgccggt
     2341 ggtcgccgtt gacccggcgc cgggcgagac gatagagctg ccgccgctcc agggctactc
     2401 aggcgcggtc ggcgtcgtga tgcggcgcga gggcggcgtg ggcggccccg cgctcgaacg
     2461 cgccgtcacg ctcaccctgc ccgcgcccgc cgccgaacac cgccgccgcg tgtgggagcg
     2521 gcgtctcggc acacaggccg gcgcggacct gccggacatc gtcgagcgct tcctgctgcc
     2581 gctcggcaac gtcgagcgcg cggcgggcgt ggcggccacc tacgcgctgc tcgaacggcg
     2641 cgagaccgtg acgaccgccg acgtgcagct cgcctgccgc tcgctcaacc ggcagaagct
     2701 cgacacactg gcgcagcggc tcgaagtcgc gggcgggtgg gaagacctcg tcgtcaacca
     2761 cgtcaccgcg tcgcagctcg acgagcttga gcgccgctgc cgccaccgcg agcgcctgct
     2821 ctcgaacctc ggcgcgggct tccgcaactc gctcaactgc ggcgtgcgcg cgctcttcaa
     2881 cgggccttcc ggcacgggca agacgctcgc cgccaaagtc ctcgccgccg tcttgcagtc
     2941 ggacgtgtac cgcgtcgatc tggcctccat cgtcaacaag tacatcggcg agacggagaa
     3001 gaacctcagc caactgctcg cgcgcgccga agagctggac gtgatcctgc tcatcgacga
     3061 gggcgactcg ctgatgacgg ggcgcaccga cgtgaagtcg gccaacgacc gttacgccaa
     3121 cctcgaaacc aactacctgc tgcaacgcat cgagacgtat cagggcatcg tcgtcgtcac
     3181 gaccaacgcg ggcaaccgca tcgaccaggc gttcaagcgg cgcttcgacg tgctcatcga
     3241 cttccagccg cccgacccgc gcgagcgcct gctcatctgg cgccaccacc tgcccgccgc
     3301 gcaccgcgtc tcggacgcgc acctgcaaag gctcgccggc cactgcgcgc tgaccggcgg
     3361 gcagatacgc aacgccgcgc tgcacgccgc gctgctcgcg gtcgtggccg ggcgcgacgg
     3421 cgaagtggaa gacgccgacc tcgaagccgc cgtcagccgc gagtaccgca aggccggcgc
     3481 ctcgtgcccg ctcgggcagg cgcccgtcag gaacgggcac cacgccgcgc tcacgcagtt
     3541 tctcgcggag atcggttgag caggtcgccc gtcgcgcgct cgcgcgccgg ctggaggcgg
     3601 caagcgccgc aacgaagcaa cgatgcagat cgcccagacc aaatcgcgcg gcagggcggg
     3661 cggggagacg acggactccg ccgcgccgga ttcgctcgcc gcgacgggcg acgcgacacg
     3721 gatgcccgca tacgcgctcg ggcaggacgc cgccccgacg atcccgctgc tcggcggcgg
     3781 cctcgtgcag accaagctgg cgataggcag cgccggagac ccctacgaag gcgaggccga
     3841 cgccgtcgcc gcacacgtca ccgccgacaa ggaaggcgcg tcgccgccct ccatctccaa
     3901 cgtcacggcg cgcgcgctcg ccccgtccgc ccccgccgtg ggaggcggcg gcgaggccgt
     3961 ccagaaggaa tcggaaccgg cccccgccgc cgatgaagag gagcaggaag aagagtccgc
     4021 cctccccgtc cagcgccaac ccgccgcgga agaagagcag gaacccgtcc aactcgaaac
     4081 ggacgaagaa gagccggacg agtccgtcca gcgccagccg caggaagacg agcaggaacc
     4141 cgtccagctc gtccagcggc aggcggcagt cgaagaggaa gaacccgccg tcgaacagca
     4201 ggaggaagaa gcggagcctg tccagacgaa agcgaagccc ggacgcggcc cgcgtgacga
     4261 agaggaggaa gagaggaaag agcgagagcg aagggccgag ggcgcgatca acaaccggca
     4321 gggcggctcg cccctcgacg aagagacgcg ctcgcacgtc gagcggcgca tgggccagga
     4381 cttctcggac gtgcgcgtcc acgacgacgc gcagtccgcc gacgccaaca aggcactcaa
     4441 gtcgcgcgcc ttcgccaacc gcaaccacat cttcctcggc gcgggcgaga gcaggaaaga
     4501 caaacggctg ctggcgcacg aactcacgca catcgtccag cagggcggcg tctctcgtcg
     4561 ccctccgcgt aacggcgcgg gcacagccga gccgcctccg tccgccggcc cgcaacccgg
     4621 cggcgtgacg aagcctgccg cgggaggcca gcaaccgccg tccgccctgc ccgccgctca
     4681 aactcccgcg cttgccggga cggcccccgc gggcaccacc ccgacagccg ccaaaactcc
     4741 gccgccggta actgccgcgc cgtccggtaa agcccagggc gccactgcgc cgcccgcggc
     4801 gacagcagcg cccggcgccg ctcagacggg tctcaccggc gtcatcaccc ctgcgaagcc
     4861 tgccgatcta gccgccccgt ccggcgtggc cccggctccc gccgcgggcg cggaaccgaa
     4921 agccgcaggc gccgaagtca agcccgccga ggctcaagcc ggtgcggcgg gagcgacgcc
     4981 ggagaccgcc accgggaaac ccgccggcga agcaggtgcg acacccgagg ccgaagccgg
     5041 gaaggcaccc gccgagggag ccgccgcaga ggccgcaggt gaagctcagg ccgccccagc
     5101 cggtggggtg cccgaggagc ttggcggggc tgccggcgga gccatggccg agggcggcgc
     5161 ggctcccgct gtcgaaggcg ggaaggaagc cgccgcgggc ggaggcggcg cggacgtgag
     5221 cgccgaaggc ggcgaggccg aaggcggcgg gagtgaagag gaaggcgccg ccgagatgga
     5281 agagcgcatg gccgccgccg cgggcgagaa gaagtccgag ggagagggtg cgggcgcagg
     5341 cgagggcggc ggcacagccg aagcggcggg cgaagggtct gccgcagaag aacccgaacc
     5401 ggaaggcgag aagaccgggg aagagcctga gcagttggcc gtaccggagc cggaacaggc
     5461 ggcggcggca gccggcggcg agggcggcgg cggcgaacct cccatgcccg ccgaacaaag
     5521 cgacaccatc tcggaagaag ccgcggcgga cgccctctct ggcggaggcg gcgatatgca
     5581 ggaggcgggc gaggccgtca aggaggccaa cgccgcggaa ggtgaggagg aagaggccgc
     5641 cgcgcctgcc gccgcgggcg cgtccgagga ggccgaagtc ggagccgcgg gcggcggggc
     5701 cgaagcccct gtcggagagg ccgcccccgc cgaaactccc ggcgcggagg ccgcggcgga
     5761 aggtgaaagc gaagaggccg gcgccgctcc cgaagccgga gcggagagcg gagagctggc
     5821 gcccgccgaa cgcgacgccg cgatgtcttc gctggccgcg ggcggcggcg gcgagggcgg
     5881 cggcggtggc ggcggtggcg gcggcggcgc catccccgac aaggaggagc cggaagcgcc
     5941 cgacgtgt
//
LOCUS       DBNGOH010000325         3477 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3899422, whole genome shotgun sequence.
ACCESSION   DBNGOH010000325 DBNGOH010000000
VERSION     DBNGOH010000325.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3477)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3477)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3477
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3899422"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            163..969
                     /locus_tag="AC159P_06215"
     CDS             163..969
                     /locus_tag="AC159P_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HIR3695085.1"
                     /translation="MDREAKKIRTLIVDDEPLARQSIRLLLKADAEVEIVGEAGSGAE
                     AVKAIRRHSPDLVFLDIQMPEMDGFGVIENIDAERMPVVVFVTAFDRYALKAFEVHAL
                     DYLLKPFSDARFEKALRQAKAQIGQREISHLSQRLLALLEDRDGRHAPPPTARAASYL
                     SRLMVKSAGRVFFLKADEIDWIEAADYYVKLHVGRKSHLLRESLSELETKLDPEKFLR
                     IHRSSIVNLDRVREMHPLPGSEYAVLLHDGTSLKLSRARREHLQRLLEKQ"
     gene            complement(1136..1774)
                     /locus_tag="AC159P_06220"
     CDS             complement(1136..1774)
                     /locus_tag="AC159P_06220"
                     /inference="COORDINATES: protein motif:HMM:NF012521.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HIR3695086.1"
                     /translation="MLTRILLIRHGQSEGNAERRFGGHSATPLSALGRAQAEATARAL
                     RDEAVTAIYSSDLLRAVETAEPLARATGLEVRSTDAFRERSVGRMEGLTFEDAAEQFP
                     EEYAALLRRDFDHVLLGGESYRQLLDRAAAELDRAVEANRGGTVALFSHTGTICILAL
                     HLMGALDAPTLRPVWVASANCGVTRFHIQHGGLIRVLAVNDTRHLGGLGEKK"
     gene            2102..>3477
                     /locus_tag="AC159P_06225"
     CDS             2102..>3477
                     /locus_tag="AC159P_06225"
                     /inference="COORDINATES: protein motif:HMM:NF024907.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP repeat domain-containing protein"
                     /protein_id="HIR3695087.1"
                     /translation="MMKVKTSLLTLILCSTAMLPTAVLSSPAAVAAACTALNFKAART
                     YAAGTNPRSVAHADFDADGDLDLAVANYGSHNVSVLDGDGAGNFAPPSNVQAGTNPRE
                     VTVADFNGDGRPDLITANERSSSLSVILNAGGGAFGAAQTVGVGAFPHSVAVADYNSD
                     GKLDLVVGYGGSSFISMLLGDGAGNFAPPTQHSLTSHIGAHQLGAADFNADGKTDVFV
                     VLASGGADNLAVLLGDGAGGLGEPKISLGGSNAVHGIAGDFNADGKVDVVTADSRPTS
                     TGPVALMLGDGAGRFAFPLQFTAGGNPMAVAAGDFDGDSKTDLVAANEISNGVSFLAG
                     TGGAGADAFAPAKHFGVGTDPTALTVADFNRDGRPDIVSANHASNDVSVVLNRGGGDF
                     AATLNLPVRLNPWGMASADFNGDGRADLATANSSDNSISLLFAQGAGGFAPSVRVAAP
                     GPRWLAAA"
BASE COUNT          578 a         1203 c         1152 g          544 t
ORIGIN      
        1 ccaccgcttc gacgtgcgcg accgcaacga aggcggcgtc gaggcgcggc tcgtcatccc
       61 cttccggaga aatgatgaat gcggaatgcg gaatgatgaa tgaaaaccga aagcctttgt
      121 cttttcattc cgcattccgc attccgcact ctgcattttc ttatggatag ggaagcgaag
      181 aagatcagga cgctcatcgt ggacgacgag ccgctggcgc ggcagagcat tcgcctgctc
      241 ttgaaggccg acgcggaggt cgagatcgtc ggcgaggcgg ggagcggggc cgaggccgtg
      301 aaggcgattc gcagacactc gcccgacctc gtcttcctcg acatccagat gccggagatg
      361 gacggcttcg gcgtcatcga gaacatcgac gccgagcgga tgcccgtcgt cgtcttcgtc
      421 accgccttcg accgctacgc gctcaaagcc ttcgaggtgc acgcgctcga ctacctcctc
      481 aagcccttca gcgacgcccg cttcgagaag gcgctccggc aggcgaaggc gcagatcggc
      541 cagcgcgaga tcagccacct cagccaacgc ctgctcgcgc tgctcgaaga ccgcgacggg
      601 cggcacgctc cgccgccgac cgcgcgcgcg gcgagctatc tgtcgcggct gatggtcaag
      661 tccgcgggcc gcgtcttctt cctcaaggcc gacgagatcg actggatcga ggccgcagac
      721 tactacgtca agctccacgt cgggcgcaag agccacctcc tgcgcgagtc gctgtccgag
      781 ctcgagacga agctcgaccc cgaaaaattt ctccgcatcc accgctcctc catcgtcaac
      841 ctcgaccgcg tccgcgagat gcacccgctc cccggcagcg agtatgccgt cctcctccac
      901 gacggcacct cgctcaaact cagccgcgcc cgccgcgagc acttgcagcg actattggaa
      961 aagcagtagg cagcgggcag cgggcagcag gcagaaggca gtaaaagcag aagagcgagg
     1021 aggcttgaga ggtaaagtgc aaagcctctt cgctcttcaa gcgataaagc aagaggcttg
     1081 agacgtggcc gctcaagcct cttgctctta ctgcccgctg cctgctgcct gctgcctact
     1141 tcttttcgcc cagtccgccg agatggcgcg tgtcgttgac ggcgaggacg cggatgaggc
     1201 cgccgtgctg gatgtggaag cgtgtgacgc cgcagttggc cgaggcgacc cagacggggc
     1261 gcagggtcgg ggcgtcgagc gcgcccatga ggtggagcgc taggatgcag atggtgcctg
     1321 tgtgcgagaa gagcgcgacc gtgccgcccc ggttcgcctc gacggcgcgg tccagttcgg
     1381 cggcggcgcg gtcgaggagt tggcggtagc tctcgccgcc aaggagcacg tggtcgaagt
     1441 cgcgccgcaa gagtgcggcg tactcttcgg ggaactgttc cgccgcgtcc tcgaacgtca
     1501 agccctccat ccgcccgacg ctccgctcgc ggaaggcgtc cgtgctcctg acctcaaggc
     1561 ccgtcgcgcg cgccagaggc tccgcggtct cgaccgcgcg tagcaggtcg ctcgaataga
     1621 tggccgtgac ggcttcgtcg cgcagggcgc gcgccgtcgc ttcggcctgc gcccgcccga
     1681 gcgcgctcag cggcgtcgcc gagtggccgc cgaaccgccg ctcggcgttc ccctctgact
     1741 gcccgtggcg gatgagtagg atgcgcgtta acatctgccg agaatgtagc aagaagaggg
     1801 cggcgcgaaa aggtcgccgc cggggaatga ggcggcgtgg atggtgtttg agcgcgccgc
     1861 cggtgtcttg ctataatcgg cggcgtcaat ccgcgagtcg tctcctccat cgcgccgacc
     1921 gcgactcgaa caacaacagg cgactcggcc cacgaccatc gtccacatgc cggcttgccg
     1981 gagaccgatc acgaattagc aattacgtgt gacggttgag cccggggcac tgccctacgg
     2041 tttgccgtgc gccctgctgc gcgatgggcc gcccctgttc acgcaaagag ttgaacgaag
     2101 catgatgaaa gtgaagacct cgctcctgac gttgatttta tgctcgacgg cgatgctgcc
     2161 gacggcggtg ctgtcgtcac cggcagccgt cgccgcggcc tgcacggcgc tgaacttcaa
     2221 agcggcacgg acatacgcgg cgggcaccaa cccccgttcc gtcgcacacg cggacttcga
     2281 cgccgacggc gacctcgacc tcgccgtcgc caactacggc tcgcacaacg tctccgtgct
     2341 cgacggcgac ggcgcgggca acttcgcgcc gccctccaac gtgcaggccg ggacgaaccc
     2401 cagagaggtc acggtcgccg acttcaacgg cgacgggcgc cccgacctca tcacggcgaa
     2461 cgagcgttcg agcagcctct ccgtgatctt gaacgcgggc ggcggggcgt tcggcgcggc
     2521 gcagaccgtc ggcgtcggcg cgttcccgca ctcggtcgcg gtcgcggatt ataactcgga
     2581 cggcaagctc gacctcgtgg tcggctatgg cggctcaagc ttcatctcga tgctgctcgg
     2641 cgacggggcg ggcaacttcg cgccgccgac ccagcactcg ctcacttccc acatcggcgc
     2701 gcatcagctc ggcgccgccg acttcaacgc ggacggcaag acggacgtgt tcgtcgtgtt
     2761 ggcgtcgggc ggcgccgaca acctcgccgt actgctcggc gacggggcgg gcggcctcgg
     2821 cgagccgaag atttcgctcg gcggctcgaa cgccgtccac ggcatcgcgg gcgacttcaa
     2881 cgccgacggg aaagtcgatg tcgtgacggc ggactcgcgt ccgacctcga ccgggccggt
     2941 cgcgctgatg ttgggtgacg gcgcgggccg cttcgccttc cccttgcagt tcacggcggg
     3001 gggcaacccg atggccgtgg cggcgggcga cttcgacggc gacagcaaga cggacttggt
     3061 cgcggcgaac gaaatctcca acggcgtctc gtttctggcg ggcacgggcg gcgcgggcgc
     3121 cgacgccttc gcgcccgcga agcacttcgg cgtggggact gacccgacgg cgctcaccgt
     3181 cgcggacttc aaccgcgacg gcaggcccga catcgtctcg gccaaccacg cctcgaacga
     3241 cgtttcggtc gtgctcaacc ggggcggcgg agacttcgcc gccaccctca acctccccgt
     3301 caggctgaac ccgtggggca tggcctccgc ggacttcaac ggcgacgggc gcgccgacct
     3361 cgccaccgcc aacagcagcg acaacagcat ctcgctcctc ttcgctcagg gcgcgggcgg
     3421 cttcgcgccg tctgtgcgcg tcgccgcgcc cggcccgcgc tggctggccg ccgccga
//
LOCUS       DBNGOH010000326         3055 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1333597, whole genome shotgun sequence.
ACCESSION   DBNGOH010000326 DBNGOH010000000
VERSION     DBNGOH010000326.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3055)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3055)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3055
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1333597"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..738
                     /locus_tag="AC159P_06230"
     CDS             <1..738
                     /locus_tag="AC159P_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013179164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR3695088.1"
                     /translation="TAFEVVMDYKGKTAIVTGSTKGIGRAIAEALSRAGMNVCVSARK
                     IEEIEQTVRELEGAGEGGVTGAACDVRDYEEVRALFEHAVAEFGGVDVLVNNAGVGIY
                     KPVEEISPEEFRAVLETNLFGVFYCTREAIPLMKQRGGGYIVNISSLAGTNAHPRMAA
                     YNASKFGLNGFSEALMQEVRHDGIKVSYVMPGSVNTEFGGDTVSAEKSWQLQPEDVAR
                     VVTALLAHDERSLPSRVEIRPSRPPKK"
     gene            893..1924
                     /locus_tag="AC159P_06235"
     CDS             893..1924
                     /locus_tag="AC159P_06235"
                     /inference="COORDINATES: protein motif:HMM:NF015506.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3695089.1"
                     /translation="MKYFLTRAVAVLVVCGASMAQGHVVSAQAGGSKPAAGAQGESPE
                     LAEADRLSAEVVKLYQAGRYEEALPLAQQVVGVREKGLGRTHKLVAVSLLNLGAVQQK
                     LARYEEARKAYRRSLAIYEEDAAANARQVAQVLDSLAFIERDIGRAIELHERSRELKA
                     KAAGAESRDAAATLYPLGHLYELRGELDKAAHSFRRFVAVGEKVKLGNASDVGAAHFR
                     LSCLSRKKGEEAEAQKHEARGRESFAEIPEGASLVDAGVVNGKAISKPQPSYPEEAKR
                     QRAQGEVVVKILVGESGGVLAACAETGPALLRRASEVAAYGARFTETTVGGKPVKVTG
                     LITYRFVLQ"
     gene            1935..2384
                     /gene="dtd"
                     /locus_tag="AC159P_06240"
     CDS             1935..2384
                     /gene="dtd"
                     /locus_tag="AC159P_06240"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014464963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - peptidyl-tRNA hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HIR3695090.1"
                     /translation="MRAVVQRVTRASVRVGGETVGEIARGLVVLLGIARDDTRADVEY
                     LVEKVAAVRVFDDAEGRMNRSVADAGGGLLVVSQFTLYGDVRRGRRPSWIDAAPPEVA
                     EPLYELFVAEARARVPAVATGSFRREMEVELVNDGPVTILLDSRKLF"
     gene            2511..>3055
                     /locus_tag="AC159P_06245"
     CDS             2511..>3055
                     /locus_tag="AC159P_06245"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIR3695091.1"
                     /translation="MCACAALSALFAACGGGPQNANVADPDEVNPNVKTGVKPEPDAE
                     AAVIEMEDPAYGRIVLELYPNIAPKMVERFKTLAREGFYNGTAFHRVEPRANVIQGGD
                     PNSKDADPSNDGAGGSPYPDLTAEFSDIPYEAGVVGAARTNDPDTANAQFFITLGRVP
                     GWDGSYTVFGRVIDGLNNARV"
BASE COUNT          541 a          944 c         1134 g          436 t
ORIGIN      
        1 acggcctttg aggttgtgat ggattacaaa gggaagacgg ccatcgtgac ggggagcacg
       61 aagggcatcg ggcgcgcgat agcggaggcg ctgtcgcgcg cggggatgaa cgtgtgcgtg
      121 agcgcgcgga agatcgaaga gatcgagcag accgtgcgtg agttggaagg cgcgggcgag
      181 ggcggcgtga cgggcgcggc gtgcgacgtg cgcgactacg aggaggtgcg ggcgctgttc
      241 gagcacgcgg tcgcggagtt cgggggcgtg gacgtgctgg tgaacaacgc gggcgtcggt
      301 atctacaagc cggtcgaaga gatttcgccg gaggagtttc gcgccgtgct cgaaacgaat
      361 ctgttcggcg tcttctactg cacgcgcgag gccatccccc tcatgaagca gcgcgggggc
      421 ggctacatcg tcaacatctc ttcgctcgcg gggacgaacg cgcacccgcg gatggcggcg
      481 tacaacgcct cgaagttcgg cctcaacggc ttcagcgagg cgctgatgca ggaggtgcgg
      541 catgacggca tcaaggtgag ctacgtgatg cccggctccg tcaacacgga gttcggcggc
      601 gacacggtga gcgcggagaa gagctggcag cttcagccgg aggacgtggc gcgcgtcgtc
      661 acggccctgc tcgcgcacga cgagcggagc ctgccgagcc gcgtcgagat caggccgagc
      721 cgaccgccga agaaatgatg aatgcggaat gcagaacgat gaacggaagg cagtttccag
      781 tcacggagtt tgtcttcact aatagctgac ggctgaacgc tgaaaaccga aaaccgtttc
      841 atcgttctgc attccgcact ctgcactgct ttatgttggg aggtgaagtg gcgtgaaata
      901 cttcctcacg cgcgccgtcg cggtgctcgt cgtgtgcggc gcgagtatgg cgcaggggca
      961 cgtcgtctcg gcgcaagccg gagggtcgaa gccggcggcg ggcgcgcagg gggagtcgcc
     1021 ggagttggcc gaggccgatc ggctgagcgc cgaggtcgtc aagctttatc aggccgggcg
     1081 ctacgaagag gcgctgccgc tcgcgcagca ggttgtcggg gtcagggaga aaggactcgg
     1141 gcgcacgcac aagctcgtcg ccgtctcgct cctcaacctc ggggccgtcc agcagaagct
     1201 cgcacggtac gaagaggcgc gcaaggctta caggcgctcg ctcgccatct acgaagaaga
     1261 cgcggcggcg aacgcgcggc aggtcgcgca ggttctcgac agcctcgcct tcatcgagag
     1321 ggacatcggg cgggccatcg agttgcacga gcgcagccgt gagttgaaag cgaaggccgc
     1381 gggcgcggag agccgcgacg ccgccgcgac gctctacccg ctcggccacc tgtacgagct
     1441 gcgcggcgag ctggacaagg cggcccactc cttcaggcgc ttcgtcgcgg tcggcgagaa
     1501 ggtgaagctc ggcaacgcga gcgacgtggg cgccgctcac ttccgtctga gctgcctgtc
     1561 gcggaagaag ggggaggaag cagaggccca aaagcacgag gcgcgcgggc gcgagtcctt
     1621 cgccgagatt ccggagggcg cgagtctggt cgatgcgggc gtcgtcaacg ggaaggcgat
     1681 cagcaagccg cagccgtcgt acccggaaga ggccaagcga cagcgcgcgc agggcgaggt
     1741 cgtcgtcaag attctggtcg gcgagagcgg cggcgtgctc gcggcgtgcg ccgagaccgg
     1801 ccccgccctg ctccggcgcg cgtcggaggt ggcggcctac ggcgcgcgct tcacggagac
     1861 gacggtcggg ggcaagccgg tcaaggtcac gggcctcatc acctacaggt tcgtcctcca
     1921 gtgacacgaa agagatgcga gcggtggtgc agcgcgtgac gcgcgcgtcg gtgagggtcg
     1981 ggggcgagac ggtcggcgag atcgcgcgcg ggctggtcgt gctgctcggc atcgcgcgcg
     2041 acgacacgcg cgccgacgtg gagtacctcg tcgagaaggt cgccgccgtg cgcgtcttcg
     2101 acgacgcgga ggggcgcatg aatcgctcgg tcgcggacgc gggcggcggg ctgctcgtcg
     2161 tctcgcagtt cacgctctac ggcgacgtgc gccgcgggcg gcggccttcg tggatcgacg
     2221 cggcgccgcc cgaggtggcc gagccgctct acgagctgtt cgtcgccgag gcgcgcgcgc
     2281 gcgtccccgc ggtggcgacc ggcagcttcc gccgcgagat ggaggtcgag ctggtcaacg
     2341 acggcccggt gacgatcctc ctcgacagcc gcaagctttt ctgaaagcgg ggccgccttt
     2401 tcgaagctcg aacagtttca accaacacac gccggaggga atctgtgggt aaaggtcaac
     2461 agcgggcagc agcgaagcgg attaatgcca acagggggag actgatattg gtgtgcgcct
     2521 gcgcggcgct ctcggcgctc ttcgccgcgt gcggcggcgg gccgcagaac gcgaacgtcg
     2581 ccgacccgga cgaggtcaac ccgaacgtca agacgggcgt caagccggag cctgacgcgg
     2641 aggcggcggt catcgagatg gaagaccccg cgtacggcag gatcgtgctg gagctgtacc
     2701 cgaacatcgc gccgaagatg gtcgagcgct tcaagacgct ggcgcgcgaa ggcttctaca
     2761 acggcacggc cttccaccgc gtcgagccgc gcgccaacgt catacagggc ggcgacccca
     2821 actcgaagga cgcagaccct tcgaacgacg gcgcgggcgg ctcgccctac ccggacttga
     2881 ccgccgagtt cagtgacatc ccctacgaag cgggcgtcgt cggcgccgcg cgcaccaacg
     2941 acccggacac ggctaacgcg cagttcttca tcacgctggg ccgcgtcccc ggctgggacg
     3001 gctcctacac cgtcttcggg cgcgtcatcg acggcctcaa caacgcccgc gtcat
//
LOCUS       DBNGOH010000327         3919 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2983126, whole genome shotgun sequence.
ACCESSION   DBNGOH010000327 DBNGOH010000000
VERSION     DBNGOH010000327.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3919)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3919)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3919
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2983126"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..487
                     /locus_tag="AC159P_06250"
     CDS             <1..487
                     /locus_tag="AC159P_06250"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR3695092.1"
                     /translation="ERVERLVLVSSMTAARNIVGADELRQAVASLDGAVPAEFARDFQ
                     ESTVYRQLPTDFMEGVVAASLKLPARVWRAVLEGLLADEHASRLGQIGAPTLILWGER
                     DALLKRAEQDALLSALGNAVLKVYKETGHSPHWERPEQFVGDLEAFLDHAAARERRDA
                     A"
     gene            529..>3919
                     /locus_tag="AC159P_06255"
     CDS             529..>3919
                     /locus_tag="AC159P_06255"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HIR3695093.1"
                     /translation="MSFLQTGQTLKHYAILSKIGQGGMGQVYKAEDKKLGRTVAVKLL
                     PQDKAQSDTARHRLLQEARSASVLNHPGIVTIHSIEEHEGVDFIVMEYVEGETLRAAV
                     ERGPLDLALLLDLGAQLADALAAAHSKGIIHRDIKSENILLTPRGHVKVLDFGLAKPA
                     ESVSGEVNSEAPTRLNLTGEGAVLGTVLYMSPEQTRAEPLDARTDIFSLGCVLYEAAT
                     GRLPFGGASLLSVMQAIVSKEPPAPSSLRAELPFEFDLIIERALAKDKARRYSSAAEL
                     ADALRVLNGTATTSLRVSAEQRVAPAEDAGRDVPVGRAPELERLDGLLRRAVEGVGRV
                     AFVTGEPGIGKSALVGEFLRRARREHTSIVVARGRCVEQYGTGEAYLPFLDAVGSLLT
                     GASRERIVALLRTYAPTWCLQFPAAFVSSLALEKLQQETAGATKERMLREMGDLLAAL
                     AAQSPVALVLEDLHWADPSSVDLLRHLSHRASDQRLLVVGTYRPGDVELNDHPLKSFM
                     LETAAHSLCDEIALGSLTRRHVAGYLDSRFAPNDFPAELTALVHAKTEGHPLFVTSLA
                     QFLVERGDIAQRGSVWTLARPLAEMSLEAPENVRSMIRKKLESLGDEDRRALQYASVE
                     GEEFLSTVLAGLLGTDDLELEERLAHVERAYRLIETAGEEELPDGSLATRYRFAHALY
                     QNVLYGDLVSKRRRLLHRQAGEQLERHYGKQSARIAAQLAVHFERGHDHARAVEYLAR
                     AGDNAAKLYASSEAADNYGRAIELADKLPDTERAEPLINLYQKRGRARLTLGRFGEAV
                     DDFTEMLNEARRTGSLEQESAALNALSMTLFYSHRLDEITERADEILRAAERAQSDAL
                     RIEAMQVLALKHLGSGDLAEAKRMLDEIIPCARRLGHKSVLLTGLAWRGILHFFQTEY
                     AEAEAMLLEARGLAEELHDAFLLLEGYFVLGMVRGNMGRMSEALSTFEEGLATAERYG
                     DQFWSTRIPNCIGWIYRELQDFEKAVEYDQHGLEVGRRRGVLEAQANSLINLGIDHGH
                     SGRGDETASAFREVEQIFRRDAWFRWRYQIRLHAGACEHQLKLGDLARAEEHAHKLLE
                     TATHYEAHKYVAVAHKLLAEVALARRDLPKASAELDAALSEL"
BASE COUNT          634 a         1432 c         1308 g          545 t
ORIGIN      
        1 tgagcgcgtc gagcggctcg tcctcgtcag ctcgatgacc gcggcgcgca acatcgtcgg
       61 cgcggacgag ctgcggcagg cggtcgcgtc gctcgacggc gcggtgcccg ccgagttcgc
      121 gcgcgacttt caggagagca ccgtctaccg gcagctcccc acggacttca tggagggcgt
      181 cgtggctgcg agcttgaaac tgcccgcgcg cgtctggcgc gcggtgctcg aagggctgct
      241 cgcggacgag cacgcgtcgc gtctcggcca gataggggcg ccgacgctga tcctgtgggg
      301 cgagcgcgac gcgctcttga agcgggccga acaggacgcg ctcctgtccg cgctcggaaa
      361 cgccgtcctc aaggtttaca aggagaccgg ccactcgccg cactgggagc ggcccgagca
      421 gttcgtcggc gacctcgaag ccttcctcga tcacgcggcg gcgcgggagc ggcgcgacgc
      481 ggcgtagtcg gtgggaaggc accgccgccg cccggaggag acaccttgat gagcttcctg
      541 caaacaggtc agaccctcaa gcactacgcc atcctgagca agatcgggca gggcggcatg
      601 gggcaggtct acaaggccga ggacaagaag ctcgggcgca cggtcgccgt caagctgctg
      661 ccgcaggaca aggcgcagag cgacacggcg cgccaccgcc tgctgcaaga ggcgcgctcc
      721 gcttccgtcc tcaaccatcc gggcatcgtc accatccact ccatcgaaga gcacgagggc
      781 gtggacttca tcgtcatgga gtatgtcgag ggcgagacgt tgcgcgcggc ggtcgaacgc
      841 ggcccgctcg acctcgcgct cctgctcgac ctcggcgcac aactcgcgga cgcgctcgcc
      901 gccgcccact cgaaaggcat catccaccgc gacatcaagt ccgagaacat cctgctgacg
      961 ccgcgcgggc acgtcaaggt gctcgacttc gggctggcga agccagccga aagtgtctcg
     1021 ggcgaggtca actcggaggc gccgacgcgg ctcaacctga cgggcgaggg cgctgttctc
     1081 ggcaccgtcc tctacatgtc gcccgagcag acgcgcgccg aaccgctcga cgcgcgcacg
     1141 gacatcttct cgctcggctg cgtgctctac gaagccgcga cgggccggct ccccttcggc
     1201 ggcgcgagtc tgctctcggt gatgcaggcc atcgtctcga aggagccgcc cgcgccgagc
     1261 agcctgcggg ccgagctgcc cttcgagttc gacctcatca tcgagcgcgc gctggcgaag
     1321 gacaaggcgc gccgctactc ttcagccgca gagctggccg acgcgctgcg cgtgctgaac
     1381 ggcacggcga caaccagcct ccgcgtctcc gcggagcaac gcgtcgcgcc cgccgaggac
     1441 gcgggacgcg acgtgcccgt cgggcgcgcg cccgagcttg aaaggctcga cgggctgctg
     1501 cgccgcgcgg tcgaaggcgt gggccgcgtc gccttcgtca cgggcgagcc tgggataggc
     1561 aagagcgcgc tcgtcggcga gttcctgcgt cgcgcgcggc gcgagcacac ctccatcgtc
     1621 gtcgcgcgcg ggcgctgcgt cgagcagtac ggcacgggcg aagcttattt gcccttcctc
     1681 gacgcggtcg gctcgctgct cacgggcgca agccgcgagc gcatcgtggc gctgctgcgc
     1741 acctacgcgc cgacgtggtg cctgcaattc cccgccgcct tcgtctccag cctcgcgctg
     1801 gagaagttgc agcaggagac ggcgggcgcg acgaaggagc ggatgctgcg cgagatgggc
     1861 gacctgctcg ccgcgctggc cgcgcagtcg cccgtcgcgc tcgtactcga agacctccac
     1921 tgggcagacc cttcgagcgt cgatctgctg cgccacctct cgcaccgcgc ctcggaccag
     1981 cgcctcctcg tcgtcggcac ctaccgcccc ggcgacgtgg agctgaacga ccacccgctc
     2041 aagtctttca tgctcgagac ggcggcgcac agcctctgcg acgagatcgc gctcggctct
     2101 ctgacgcggc gccacgtcgc gggctatctc gactcgcgct tcgcgcccaa cgacttcccc
     2161 gccgagctga ccgcgctcgt ccacgccaag accgagggcc acccgctctt cgtcacgagc
     2221 ctcgcgcagt tcctcgtcga gcgcggcgac atcgcgcagc gcggctccgt gtggacgctc
     2281 gcgcgcccgc tcgcggagat gagcctcgaa gcgcccgaga acgtgcgcag catgatccgc
     2341 aagaagctcg agtctctggg cgacgaggac cgccgcgcgc ttcagtacgc gagcgtcgag
     2401 ggtgaggagt ttctctcgac cgtgctcgcc ggactgctcg gcacggacga cctcgaactc
     2461 gaagagcggc tcgcgcacgt cgagcgcgct taccgtctga tcgagaccgc gggcgaagag
     2521 gagctgcccg acgggagcct cgccacgcgc taccgcttcg cgcacgcgct ctaccagaac
     2581 gtgctctacg gcgacctcgt cagcaagcgc cgacgcctct tgcaccggca ggcgggcgaa
     2641 cagctcgaac gccactacgg caaacagtcc gcgcgcatcg ccgcgcagct cgccgtccac
     2701 ttcgagcgcg gccacgacca cgcgcgcgcc gtcgaatacc tggcgcgcgc gggcgacaac
     2761 gcggccaagc tctacgccag ctccgaggcc gccgacaact acgggcgcgc catcgagctg
     2821 gccgacaagc tccccgacac ggagcgggcc gaaccgctca tcaacctcta ccagaaacgc
     2881 gggcgggcgc ggctcacgct gggccgcttc ggcgaggccg ttgacgactt caccgagatg
     2941 ctcaacgagg cgcggcgcac ggggtcgctc gaacaggagt cggcggcgct caacgcgctc
     3001 tcgatgacgc tcttctactc gcaccgcctc gacgagatca ccgagcgcgc cgacgagata
     3061 ctgcgcgccg ccgagcgcgc gcagtccgac gcgctccgca tcgaggcgat gcaggtgctc
     3121 gccctcaagc acctcggcag cggcgacctc gccgaggcca aaaggatgct cgacgagatc
     3181 atcccgtgcg cgcgtcgcct cggtcacaag tcggtgctgc tgacgggcct cgcgtggcgc
     3241 ggcatcctcc acttcttcca gaccgagtac gccgaggccg aagcgatgct gctcgaagcg
     3301 cgcggtctcg ccgaggagct gcacgacgcg ttcctcctgc tcgaaggcta cttcgtgctc
     3361 ggcatggtgc gcggcaacat gggccgcatg tcggaggcgc tctcgacatt cgaggagggt
     3421 ctcgccaccg ccgaacgcta cggcgaccag ttctggtcaa cgcgcatccc gaactgcatc
     3481 ggctggattt accgcgagct gcaagacttc gagaaggcgg tcgagtacga ccagcacggc
     3541 ctcgaagtcg ggcgacggcg cggcgtcctc gaagcgcagg ccaactcgct catcaacctc
     3601 ggcatcgacc acggccattc ggggcgcggc gacgagacgg cctccgcctt ccgcgaggtg
     3661 gagcagattt tccggcgcga cgcgtggttc cgctggcgct accagattcg cctccacgcg
     3721 ggggcctgcg agcaccagct caagctcggc gatctggccc gcgctgaaga gcacgcgcac
     3781 aagctgctcg aaacggcgac ccactacgag gcgcacaaat acgtcgccgt cgcgcacaag
     3841 ctgctcgccg aagtcgccct cgcgcgccgc gacctcccga aggcttccgc ggagctggac
     3901 gccgccctct ccgagctgc
//
LOCUS       DBNGOH010000328        20670 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1333917, whole genome shotgun sequence.
ACCESSION   DBNGOH010000328 DBNGOH010000000
VERSION     DBNGOH010000328.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 20670)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 20670)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20670
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1333917"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(120..956)
                     /locus_tag="AC159P_06260"
     CDS             complement(120..956)
                     /locus_tag="AC159P_06260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695094.1"
                     /translation="MPKSSQKPAGSEQTASQRGKSRFGSSGRSARVVLPERPPRAVDH
                     EIVSILLGADPASLTEKSQDSGNAVPPEPGDAAQPSDPAAEAAPITISSDSTLQPTSV
                     ATPPKAVQSGGPDLAGNVSTSERVLEEPVSLRVEGVTADEEGIQAAEISAPARRHTPA
                     ATEHHAEIGARPPTRGMSAQDNPITSFEEFADRWRHGLRKGQLKICEVIYQKTYAVGQ
                     TECVTSFSELGRLSGLKMRQCFNVIAQLEALKFVERARSEGTSNKKDQGSVIRFYLFP
                     FQ"
     gene            complement(970..1776)
                     /locus_tag="AC159P_06265"
     CDS             complement(970..1776)
                     /locus_tag="AC159P_06265"
                     /inference="COORDINATES: protein motif:HMM:NF018290.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HIR3695095.1"
                     /translation="MRAVISVANQKGGVGKTTTAAAIAADLALRKYRTLLVDADPQAN
                     ATTHFLLPGQYEHTIYDVLVEKDVNASSPIEAAVVTTDIETLDLVPSRIRLSAFDKEP
                     PISIHRLKAKLETIYDEYDFVIIDTPPSLGQILMAVLIASTHIIVPVSANPLSHDGLD
                     DLVGTIEQLRGVNKDLKILGVVTTLFDTRTALSGASHEETRKRFDKLVFDTIIHDNVK
                     LAECPGHNKPVQLHAPNSRGAQLYGALTDEVLQRLKLPLSKTNLRAVRGK"
     gene            complement(2137..2919)
                     /locus_tag="AC159P_06270"
     CDS             complement(2137..2919)
                     /locus_tag="AC159P_06270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695096.1"
                     /translation="MRATRRRTRTPDPLTRPEAVRDICEEVRSRMHGVISNSERHLIR
                     FLYAVRHVERRPATDTRRGRPSRWRREDLISAAGHLRAVLQRETSGRLSLNSFVGQYL
                     QILDFPSDVQGALADGRINLQEAAQLARLTGESLGCSAAEARRTRAEILQAHLTVQGS
                     QTRLRSRVKEMLGEASAAQVSSEGVAEVVLKVDELLEVDPADTRHLFFEEMKRLFFAM
                     REIEADDLNEEILNDFLHAVDQVSNVLSRIEKKRRERLSAQS"
     gene            3172..4173
                     /locus_tag="AC159P_06275"
     CDS             3172..4173
                     /locus_tag="AC159P_06275"
                     /inference="COORDINATES: protein motif:HMM:NF012798.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HIR3695097.1"
                     /translation="MNDDQRLVLVGREDGVALRNAIALWADATTDAGSDRRSDLLRDK
                     TRAVTTFFGFAAKHAAEVLPLDVKKWQVALETSGLTRATVYTRLSLLSSFFEWAMREP
                     AVGAFLASNPVRLARPKAPRAYQTESTKSLTDDELRALLASVRERAARPAAVTAKRDY
                     ALLLLYVLTGMRRQELISLRGRDVEFREDALIIRGRVKGGDYIGREVRDPAVRAALLN
                     YLEASGRLNALEGERPLWTRHDRAGQPGAPLTSHALVRNLKVYARESGIKEFHLHQIR
                     HTYARLVAEKTGSIVETQDALGHKNPATTRVYVQRIAVKQDKHSEQIARLLDLEDVG"
     gene            complement(4112..4393)
                     /locus_tag="AC159P_06280"
     CDS             complement(4112..4393)
                     /locus_tag="AC159P_06280"
                     /inference="COORDINATES: protein motif:HMM:NF012257.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HIR3695098.1"
                     /translation="MSTDKVAALGRTPLFKELDAGLLRALAERAVERRFRKGELLFVA
                     GEEARGLYVIVEGSVRAFRKSADGRESECVTQHLPNQEVARSARCAYPA"
     gene            complement(4476..6086)
                     /locus_tag="AC159P_06285"
     CDS             complement(4476..6086)
                     /locus_tag="AC159P_06285"
                     /inference="COORDINATES: protein motif:HMM:NF024765.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HIR3695099.1"
                     /translation="MCQNNETRSTPRKRTMPCAALLFMLISALASAASAQTTAGISLV
                     NDQPKPAAPSDERPTPAARKQGLPAKAPKQLKLGKVNVSGSLRLRAESYGWFETPGFE
                     DEYTFGAAQLRLSLGQQREKWDWLVEGQFTGFVNLPERAGAVAPQGQLGLGASYSAAN
                     GRQDASAFLKQAYVRAKGVFGDRRGSLKAGRFEFVEGAETTPKDATLAAIKRDRLAHR
                     LIGNFGFSHVGRSFDAVQYVRENKSSNLTLVGGRAVEGVFQLRALKELDVDFWYGAYT
                     KPLHAARVESELRLFGLHYHDGRGALKTDNRTATARRADAENIRVNTVGGHYIVAART
                     SRGTFDALAWGVGQFGAWGTQSHRAGAIAFEGGFQPGGALSKVKTWVRAGYFRGTGDG
                     DPSDDRHTTFFQMLPTPRVYARFPFYNLMNNEDVFAQLQLKPHARLSLRADVRRLRLS
                     NANDLWYAGGGAFQETSFGYQGRPSGGRRSLGTLFDLSADVTLTPTTTLTLYGAGVRG
                     GGVQAGVYPAGGVNPTARLLYVELTRRF"
     gene            complement(6136..7359)
                     /locus_tag="AC159P_06290"
     CDS             complement(6136..7359)
                     /locus_tag="AC159P_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015112 - nitrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3695100.1"
                     /translation="MGETFRPGAARALFFSTTAFAVSFAVWGLIAALAPTFTQIYQLS
                     ATSKSLLIAVPVLLGSIGRLPAGMLADRFGGRRVYAALLILSALPAVAIGFSTSYTQL
                     LLLGLFLGLAGTTFPVGVGFTSKWFAAEKQGTALGVYGMGNIGQSVAVFGAPVLAAYA
                     FGGDWRPVFFAFAALAFLWGVVFYLFARDAATTAKPKTLSENLSVLRREPMAWVLSLF
                     YFLTFGGFVALGIYLPTLLKDLFGLTATDAGARTAGFVLLATLMRPVGGWLADRIGGV
                     RVLVYVFAAVAGLGLLMGCPNMLTFTVGALGAAAALGLGNGAVFKLVPQFFPKETGTV
                     TGLVGAFGGLGGFFPPLALGLIHDATGGYAWGFVFLACFALACLAVHYFTLVRPGGGA
                     RPTKVTLGGPQAALR"
     gene            complement(7554..7760)
                     /locus_tag="AC159P_06295"
     CDS             complement(7554..7760)
                     /locus_tag="AC159P_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008940403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3565 domain-containing protein"
                     /protein_id="HIR3695101.1"
                     /translation="MKRRITAYDHDDEGHWRAILDCGHPQHVRHDPPLVVREWVLTEE
                     GRASRLGYELDCKRCDEEDAEGAV"
     gene            complement(7757..8107)
                     /locus_tag="AC159P_06300"
     CDS             complement(7757..8107)
                     /locus_tag="AC159P_06300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695102.1"
                     /translation="MSVRFNSGEDAQARQRARATQTVYPVGEAPAGRRRTGRGRTAVE
                     QARLVMLVMINVAQLWILAATVEAALAHSYGELLPLVIASGVCWLISLTIILWWRPAS
                     RRHTSTGYIREGGR"
     gene            complement(8091..8693)
                     /locus_tag="AC159P_06305"
     CDS             complement(8091..8693)
                     /locus_tag="AC159P_06305"
                     /EC_number="7.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012575.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="QcrA and Rieske domain-containing protein"
                     /protein_id="HIR3695103.1"
                     /translation="MSSENASNEFTSFGIADSPRSDAHSDESDVRRAVAPHQRDFPYE
                     RGEEAHVTRREFCNFLALTSTALFASAAGFAGKAALDSRPTESFAPAKVEGAEALAPG
                     AALNFRYPTEDDTAILVRTQDGEYHAYGQKCTHLACPVYYERRRQRLECPCHEGGFDV
                     RTGNVLYGPPPRSLDEIVLERRGAEVWAVGRKAGAHERSV"
     gene            complement(8683..9336)
                     /locus_tag="AC159P_06310"
     CDS             complement(8683..9336)
                     /locus_tag="AC159P_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR3695104.1"
                     /translation="MEETMFIDPQRCIGCRSCVAACRECGTHKGYSMIFVDYLDRSET
                     TATMPTVCMHCTEPTCALVCPADAIKMSEEGIVMSALKPRCLDCRNCVNACPFGVPKY
                     NTEMHLQMKCDMCYDRTSEGLKPMCASVCPTGALSFGKYEQIVPLRRTKPVNVHVFGN
                     QRVETRVYLMLPTDADEVRVDGCGVFEGRLGLTEQQSKTGESWIDTQVEESDKSNEF"
     gene            complement(9458..11698)
                     /locus_tag="AC159P_06315"
     CDS             complement(9458..11698)
                     /locus_tag="AC159P_06315"
                     /EC_number="1.17.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014164548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin oxidoreductase family protein"
                     /protein_id="HIR3695105.1"
                     /translation="MGKVGNLEKIVEQFGPHLHDEPHGGWRADRVIDKVVPTHCPYCA
                     VQCGMNLLVEKNHVVGFEPRYDFPVNEGRLCPKGVTAYLQVHHPDRLLYPLVKRDGMF
                     ERASWDEALDLVVSKFKELQSKHGKDAIGVYSGSSLTTEKTYLMGKFARAGLGTRYVD
                     YNGRLCMVSAAAGNNKAFGIDRAANPWDDIPLAEVLILAGANAAETFPVLNKFLWQQR
                     DNGGVWIVVDPRETPTARQGDIHLQLRPGTDVALSNGMLHVLVREKLIDESFIAARTN
                     GWEETRELALRYTPQVASEITGVPAAKIVQAALTYGRAETGMVMHARGIEHHTNGVNN
                     VLSYINLVLATGKIGAPGRGYGTITGQGNGQGGREHGQKADQLPGQRSIMNPEHRKFV
                     CGVWGLPEEELPQAGVSCVEMFHKMREGEIRGLLSICNNVMVSLPDINEVRRSLEGLD
                     FYVCIDFFMSEGARYADVVLPGTTWAEDEGVTANSEGRVVKINKAADPPGEARVDWWI
                     VQEIARRMGRGKYFPFNSPREIFEELRVASRGGNADYYGITYEKIERQNGVFWPCPAE
                     DHPGTPRLFEERFHHPDGRAKFHAVEYKPPAEVPDEEYPLILTSGRVVYQYLSGNQTR
                     RIGFLVQQCPEPYVEIHPETAARLKVSDGERVKVVSRRGEGVFPVLVVRTIRPDTIFI
                     PYHWGEQLAANQLTNAALDPTSKIPEYKACAARVEKIHTRELPILGKQRKGAERSSVT
                     RLGDSR"
     gene            11810..12805
                     /locus_tag="AC159P_06320"
     CDS             11810..12805
                     /locus_tag="AC159P_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_187388528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS701 family transposase"
                     /protein_id="HIR3695106.1"
                     /translation="MNPPKCSDDDYINFVIATPRSVSATEAAKVQGDEEQAPAHDAFT
                     RLLHRLEPDAATLWGEARTQVTLTQGILVIDDSTLDKPYARKMELVTRHWSGKHHAVV
                     SGINLITLLWTDGDRHVPVDYRIFDKKQDALTKNDHFQAMLREAHQRGFAPSCVAFDS
                     WYSGLENLKLIRSFGWIWLTRLKSNRKVNPDREGLRPVNEVEIGAAGRVVWLEGFGLI
                     RVFKIVATDGDVELWATNRVEMSDLERVRWAGYSWAIESYHRGIKQFCLIERAQVRSR
                     RAWRNHINLCLRAFLRLESHCYYKGISWYEAKTSIIREAVRAYIANPLYSLVSTA"
     gene            complement(12867..13946)
                     /locus_tag="AC159P_06325"
     CDS             complement(12867..13946)
                     /locus_tag="AC159P_06325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3695107.1"
                     /translation="MPVAETTNRRPVLLGLAASAALTVLVVVGSRNLEHYDPALFGYT
                     VASVVAFGALVYRYAVWLRRPATRVYWRRGWQLFRERKRLAKNAGSAFETVAHNIVEQ
                     RFIARRGFTRWLMHALIMWGCIISALITFPLVFGWVHFKLEGDEGYRAYLFGFPVPNV
                     MQGRSLIAWVTFHALDFTAVMVIAGCAIAIHRRLNDRGAIALQQFMLDFVPHMLLIAV
                     SVTGLMLTASSLWLEGYMYYFIALSHQALVIMTLFYLPFGKFFHIVQRPASVGVELYQ
                     RREREAPQATCPRCGTEFVGQMWLDDLKEVVATLGFDYSMPDGRTLQDFCPRCKRVMR
                     GLSYVNLPGGGERVFQGTRGEGGEQ"
     gene            14252..14566
                     /locus_tag="AC159P_06330"
     CDS             14252..14566
                     /locus_tag="AC159P_06330"
                     /inference="COORDINATES: protein motif:HMM:NF019498.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR3695108.1"
                     /translation="MKRVVIEEVEEYEDEKFVRPVLFKGERTTALLLCLKPGQSVPPH
                     RHEGFEIMLQPLRGEAEALLDGEPVTLRAGEVLFVDGANDFAPVNRGGENFSMLITLV
                     RR"
     gene            14768..15559
                     /locus_tag="AC159P_06335"
     CDS             14768..15559
                     /locus_tag="AC159P_06335"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3695109.1"
                     /translation="MTATHPGGGLKTDKMPGHWVLARLGKRVLRPGGMELTRRMLASL
                     CIGGSDDVVEFAPGMGVTARLTLASSPASYTAVERDEAAARIVKGYVVGESRRVIVGS
                     ADETGLPAASATVVYGEAMLTMQTDETKRRIVREAARLLRPGGRYAIHEMCLRDGVGG
                     EVREEVERALTGVVHHGVRPLTVTEWRRLLESEGLEVKAVETAPMALLEPARVVRDEG
                     FFRALRFAFNVLRDGDARRRVFGMRRVFRRHRGQLAAVAVTAVRA"
     gene            complement(15604..16146)
                     /locus_tag="AC159P_06340"
     CDS             complement(15604..16146)
                     /locus_tag="AC159P_06340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HIR3695110.1"
                     /translation="MTDLLLDDHAEVDTLFRDLWPEFDGGEARGVFEKLDYLWARLAV
                     HIRAEHLHLFPALLAASETRRRVEADDAPTPGEVRVAVERLREDHDFFMRELAGAVNA
                     AREMAAQDGPPELERLQQIRGRVSAVAERLVEHNRVEEQQVYLWPDALLAGAELDTLR
                     DGVRHEIENLPPRFSEGATR"
     gene            16276..16716
                     /gene="tsaA"
                     /locus_tag="AC159P_06345"
     CDS             16276..16716
                     /gene="tsaA"
                     /locus_tag="AC159P_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HIR3695111.1"
                     /translation="MHSMRPVGFVRTTFTENSQIPKGRGAKHEAEGFIEIEPEFEEGL
                     LDIEGFSHLYVIWVFHASEGYELTGVPPTDDRPHGVFATRSPYRPNPLGLTVVRLLAR
                     EGRTLRVGGVDLLDGTPVLDLKPYLSNVPEDELRRGWMDDASQV"
     gene            17137..17613
                     /locus_tag="AC159P_06350"
     CDS             17137..17613
                     /locus_tag="AC159P_06350"
                     /inference="COORDINATES: protein motif:HMM:NF012791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR3695112.1"
                     /translation="MKILLAVDGSVFGEEAAREVARRPWPEGSEVKVISAVEPVYVPA
                     AEPWGLPAGYFVNLDKLADDRARSTVEAALRLLSDREGAPLKVSTARPCGPARQVIVE
                     EAERWGADLVVLGSHGYGFWNRLLLGSVSQAVASHAPCTVEIVKRPRPAEGGVKDG"
     gene            17606..18733
                     /locus_tag="AC159P_06355"
     CDS             17606..18733
                     /locus_tag="AC159P_06355"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003954 - NADH dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006116 - obsolete NADH oxidation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR3695113.1"
                     /translation="MANVLVLGGGFGGVVAAERLAKLLVPEHRITLVSRSRRFVFYPA
                     LVRLAFGGCEPDDITLDVREALTDRRVRFIEAEVARVEPLERKVVLAHGEVAGELGYD
                     YLVFALGRRLATERVRGFFEHAHHLLTVEAALKFGEAVRGFDEGHAVVGYCEGARLAL
                     PVYETAIALSRALEARGVRGRAKITVVTPELPGERMEGDEMRRPLREALDAHGIDFWP
                     NFPVNRVTAERVHADGGPGMRYDLLMLLPPFACSSTVMDQGITDVEGYVRVAQSMRVA
                     SVERMYAVGDCVGLPGPKMAHMAVNQAEVAARNLAAEIEGRTPEAVYRHEIKLVMEEG
                     GDDSIYFHKDLEGEGAASVRRGRFWGWAKRAHEKYWQARHG"
     gene            18816..19784
                     /locus_tag="AC159P_06360"
     CDS             18816..19784
                     /locus_tag="AC159P_06360"
                     /inference="COORDINATES: protein motif:HMM:NF012791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR3695114.1"
                     /translation="MDGKMKILFAYDGSECADAALEDLRRAGLPAEAEAFVLSVADVI
                     LPPPFDEVADEALPLHLALGIRRAHEHAAQTVERARGLAEYAAGRVRTSFPGWEVRAE
                     AWASSPAWAVVMKADAWKPDLVVVGAHGHTVIGGRLILGSVSQRVLYEARCSVRVARN
                     SHRPDGSPVRVLVGTDNSPDAAAAIDAVAARAWPAGTEVRLVAVLDTVMPVSQSEGGV
                     HVLRWVEVGKEEEWGWVKDIFEASAERLRAKGLDTTVVLKKGNPKHALIEEAESWGAD
                     SIFVGARGARGIDRLLIGSVSAAAAARAHCSVEVVRPPEKPPEAGA"
     gene            19858..20304
                     /locus_tag="AC159P_06365"
     CDS             19858..20304
                     /locus_tag="AC159P_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007353261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695115.1"
                     /translation="MFLLRASAVWLVLMIAESVHGVLRMMLIAPRVGDFKARQISVFT
                     GSLLILAVAYLFVRWIRAGTTGRLLAVGLVWLALTVLFEVALGRFVMGYSWDRLASDY
                     DLARGGLMPFGLVILALSPLFAAKSRGLGKGVRASHVEGEPAARRT"
     gene            20334..>20670
                     /locus_tag="AC159P_06370"
     CDS             20334..>20670
                     /locus_tag="AC159P_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008196519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HIR3695116.1"
                     /translation="MADLNQTAEHGHSREDIRERLAAGPRHNYLRDWIYGGIDGAVTT
                     FAVVTGVVGADLSPSIILVMGFANLAADGFSMAASNYLGTKAEQEDFKHLEAVERRHI
                     ETVPEGEREE"
BASE COUNT         3608 a         6422 c         7064 g         3576 t
ORIGIN      
        1 gagagtgatt gcatatgcaa tttcaaatgc atatgtaacg agaagtgctt atgcaatccg
       61 ggaaagaggc aaaaaggcag gcgaaattga tggtagaaga aaagtaacag gactgcagct
      121 cactggaagg ggaagaggta gaagcggatg acggaaccct gatctttctt gttggaagtg
      181 ccctcggacc tggcacgctc aacgaatttc agcgcctcca gttgagcgat gacgttaaaa
      241 cactgtcgca tcttgagtcc tgagagccgg cccagctcgg agaaggaggt gacgcactcg
      301 gtctggccga cggcgtaggt tttctggtaa atgacctcgc agatcttcaa ctggcctttc
      361 cgcaacccgt gcctccacct atcggcgaac tcctcgaacg aggtgatggg gttgtcctga
      421 gcactcatgc cgcgagttgg tggccgagca ccaatctcgg catgatgttc cgttgccgca
      481 ggagtatggc ggcgcgcggg ggcggaaatt tccgccgcct ggattccctc ctcatccgcc
      541 gtaactcctt caactcttaa acttaccggc tcctccagaa ccctctcaga cgtggagaca
      601 ttcccggcaa ggtccggccc gccggactga acggctttgg gaggtgtggc cacggaggtg
      661 ggctgaagcg ttgaatcgga ggagatagta attggagccg cttcggcggc agggtcggaa
      721 ggctgggcag catcgccggg ttccggcggt actgcgttac cggagtcctg tgacttttcg
      781 gtaagcgaag cggggtcggc acccagaagt attgaaacaa tctcatggtc gaccgcgcgt
      841 gggggacgct cgggcaggac gacgcgcgcg gagcgtcccg acgagccgaa ccgactttta
      901 ccgcgctgac tcgcagtctg ctcagagccg gcgggttttt ggctggattt aggcattcag
      961 gccctgcccc tacttacccc tgaccgccct caaattggtc ttcgagagtg gcagtttaag
     1021 gcgctggaga acctcgtcgg tcagcgcacc gtagagctgc gccccgcggg agttaggggc
     1081 gtgcagttgg acgggtttat tgtgccccgg gcattccgcc agctttacgt tgtcatggat
     1141 aatagtgtcg aaaacgagct tgtcgaacct cttgcgggtc tcctcgtgcg aggcgccgct
     1201 gagggccgtg cgcgtgtcga acagcgtcgt cacgacaccc aggattttca agtctttgtt
     1261 gacgcctcgc aactgctcga tcgtcccgac caggtcgtct aagccgtcgt gagacaacgg
     1321 gttagccgac acgggcacga tgatgtgcgt ggaggcgatg agcacggcca tcaggatctg
     1381 cccgagcgaa ggcggggtgt cgatgatcac gaagtcgtac tcgtcgtaaa tcgtttcgag
     1441 cttcgccttc agccggtgga tcgagatcgg cggctctttg tcgaatgccg agagcctgat
     1501 tctggagggg acgaggtcga gggtctcgat gtccgtcgta acgaccgcgg cctcgatagg
     1561 ggatgaggcg ttgacgtcct tctcaacgag gacatcgtag atggtgtgct cgtattggcc
     1621 gggtaagagg aagtgggtcg tggcgttcgc ctgcggatcg gcatcgacga gcagcgtccg
     1681 gtacttacga agcgcgagat ccgccgcgat ggctgccgca gtcgtggttt taccgacccc
     1741 acccttctgg ttcgccacgc tgatgacagc tctcataggc actcgtctcc ttcggtcaag
     1801 gctttaggcc gctttcctgt ccgccgaaga ggcgccctga aggccgccga gcaaggcgtc
     1861 caactcttcg acactcaaca acttggcgcg ccgatcgagt tcactggtgc gacacgtaag
     1921 aagtccggcg gagataagtg ccctgagctt gtccttcgta gtctcatacc tttcacaagc
     1981 ctgcctgaac gtcagcaggc cgttgcggcg ggccatttcc tcggctaaac cattcataag
     2041 gcgcaatata ttgatagtcg tgcgggtctg tcaacccttt tcctgctcta tctagcccta
     2101 attcgcccgt tctcaccctt tttttgtctt aactgctcaa gattgtgccg acagcctctc
     2161 ccggcgcttt ttctcgatcc gggacagcac gttcgagact tgatccaccg catggaggaa
     2221 atcgtttaaa atctcttcgt tcaggtcatc cgcttcgatc tcgcgcatcg cgaagaaaag
     2281 gcgcttcatc tcttcgaaga acaggtgacg ggtgtcggcg ggatccacct ccagcagctc
     2341 atcgactttc aagacgacct cggctacgcc ttcggaagag acctgggcag cactggcttc
     2401 gccgagcatt tctttgacac gcgatcgcag cctcgtctgg gagccttgta cggttaaatg
     2461 ggcctgcaaa atctccgcgc gtgtacggcg ggcttccgcc gccgagcaac ccaaactctc
     2521 gccggtcaga cgcgcgagtt gcgccgcctc ctggaggttg atacgtccgt ccgcgagtgc
     2581 cccttggacg tcggaaggga agtcgagtat ctgaagatat tgcccgacga agctattgag
     2641 agataaccgt ccggatgttt cacgctggag gacggcgcgc aagtgaccgg cggcgctgat
     2701 caggtcttcg cgcctccagc gcgagggcct gccgcggcgg gtatcggtcg cgggacggcg
     2761 ctcgacgtgg cgaacagcgt agagaaacct gattaaatgc cgctcggagt tagagattac
     2821 gccgtgcatc cgcgaacgca cctcctcaca gatgtcgcgg acggcttcgg gccgggtgag
     2881 cgggtcgggc gtcctggtgc ggcgtcgtgt ggctcgcatg ttggctggta agcctcctgg
     2941 acgggtgacg tttcacgaac gtggcatcat acactatata tgccatactg ataggcagaa
     3001 ttgctactct actgaaacga agcttacaca aagacgctat aagtgtaagc atattgcctc
     3061 atcttaaaac ccatgataaa agaccgcagg gacagcttcg gcgcctgagt gtggggcagg
     3121 catcaacatt cgaccagctt ggacgtgcac aggagatatc aaagagaccc aatgaatgac
     3181 gaccagaggc ttgtgttggt cgggcgcgag gatggggtcg ccctaagaaa tgccatcgcg
     3241 ctgtgggccg atgcgacaac cgacgctgga agtgaccgcc gttcggacct gctgagggat
     3301 aagacccgtg cggtaaccac cttcttcggc ttcgcggcga agcacgcggc ggaggtcctg
     3361 ccgctcgacg tgaagaagtg gcaggtggcg ctggagacgt ccggcctgac gcgcgccacc
     3421 gtctacacgc ggctctcgct gctttcgtct ttcttcgagt gggcgatgcg cgaacccgca
     3481 gtcggcgcgt tcctggcctc gaacccggtt cgcctcgcca ggccgaaagc gccacgcgcc
     3541 taccagacgg agagcacgaa gtcactgacc gacgacgagt tgcgggcctt attagcgtct
     3601 gtcagagagc gggctgcgcg gcccgccgcc gtcacggcga agcgtgacta cgcgctcctg
     3661 ttactttacg ttttgacagg gatgcgccgg caagaactga tctccctgcg aggccgcgac
     3721 gttgaattca gggaagacgc tctcatcatc agagggcggg tcaagggcgg cgactacatc
     3781 gggcgtgagg tgcgcgaccc cgcggtgagg gcggcgctct tgaattacct tgaagcgagc
     3841 gggcgtctga atgctcttga gggcgagcgc ccgctgtgga cgcgacacga ccgcgcgggt
     3901 cagccgggcg ctccgctcac gtcgcacgcc ctcgtgcgga acctgaaggt ctacgcgcgc
     3961 gaatccggca tcaaggagtt tcacctccat cagatccggc acacttacgc gcgcctcgtt
     4021 gccgagaaga ccggcagcat cgtcgagacg caagacgcgc tcgggcacaa gaacccggcg
     4081 acgacgcggg tctacgtgca gcgcatcgct gtcaagcagg ataagcacag cgagcagatc
     4141 gcgcgacttc ttgatttgga agatgttggg taacacactc actctcgcgc ccgtccgcgc
     4201 tcttgcggaa ggcgcgcacc gagccctcga caatgacgta gaggccgcgc gcctcctcgc
     4261 ctgcgacgaa cagcaactcc cccttccgga agcggcgctc gaccgcgcgt tcggcgagcg
     4321 cgcgtaggag tcccgcgtca agctccttga agagtggcgt gcgaccgagg gcggcgactt
     4381 tgtcggtgct catcaggcgc gatgttggct cgacggtcgt cgcgtcgtaa aggggctgcg
     4441 gcggcgcggc ccgcgcttga gccgcttcgg cggcgtcaga aacgtcgcgt cagctcgacg
     4501 tagaggagcc tcgccgtcgg gttgacgccg cccgccgggt agacgcccgc ctgcacgccg
     4561 cccccgcgga cgcccgcgcc gtagagcgtg agtgtggtcg tcggggtgag ggtcacgtcc
     4621 gcgctgaggt cgaagagcgt gccgaggctg cgcctgccgc cggaggggcg gccctgatag
     4681 ccgaagctcg tctcctgaaa cgcgccgccg cccgcgtacc agaggtcgtt cgcgttgctg
     4741 agccgcaggc ggcgcacgtc ggcgcggagc gacaggcgcg cgtgcggctt cagttgaagc
     4801 tgcgcgaaca cgtcctcgtt gttcatcagg ttgtagaagg ggaagcgcgc gtagacgcgc
     4861 ggggtgggga gcatttggaa gaaggtcgtg tgccggtcgt ccgaaggatc gccgtcgccg
     4921 gtgccgcgga agtagccggc gcgcacccac gtcttcacct tcgagagagc gccgccgggc
     4981 tgaaagccgc cctcgaaggc gatggcgccc gcgcggtgcg actgagtgcc ccacgcgccg
     5041 aactgaccta cgccccacgc gagcgcgtcg aacgtcccgc ggctcgtcct ggcggcgacg
     5101 atgtagtggc cgccgaccgt gttgacgcgg atgttctccg cgtcggcgcg gcgggcggtc
     5161 gccgtgcggt tgtcggtctt gagcgccccg cgcccgtcgt ggtagtggag gccgaagagg
     5221 cgcagctcgc tctcgacgcg cgcggcgtgg agcggtttgg tgtaggcacc gtaccagaag
     5281 tcaacgtcca gctctttgag cgcgcggagc tggaagacgc cctcgacggc gcgaccgccg
     5341 acgagcgtga ggttggaaga tttgttctcg cgcacgtact gcacggcgtc gaagctgcga
     5401 ccgacgtgcg agaagccgaa gttgccgatg agcctgtgcg cgaggcggtc gcgcttgatg
     5461 gcggcgagcg tcgcgtcctt cggcgtcgtc tcggcgcctt cgacgaactc gaagcgtccg
     5521 gccttcaggc tgccgcgcct gtcgccgaag acgcccttcg cgcgcacgta tgcctgcttg
     5581 aggaaggcgc tcgcgtcctg ccgtccgttg gcggcggagt agctcgcgcc gaggccgagt
     5641 tggccttgag gggcgactgc tccggcccgt tcggggaggt tgacgaatcc ggtgaactgg
     5701 ccttcgacga gccagtccca tttctcgcgc tgctggccga ggctgagacg cagttgggcc
     5761 gcgccgaacg tgtactcgtc ctcgaaaccc ggcgtctcga accatccata gctctcggcg
     5821 cgcaggcgca ggctgccgct gacgttcacc ttgccgagct tcaactgctt cggcgctttc
     5881 gccggtaagc cctgcttccg tgcggcgggc gtcgggcgct cgtcagacgg cgcagccggt
     5941 ttcggctggt cgttgacgag agaaatgccg gcggtcgtct gcgcggaagc ggcggaggcg
     6001 agggccgaga tgagcatgaa gagcagcgcc gcgcacggca tcgttcgctt gcgcggcgtc
     6061 gagcgtgttt cgttgttctg acacattttg gaacctgtct gcctgatgaa agtcgggcgc
     6121 acgccgcagt acctctcagc gaagtgcggc ctgcggcccg cccagcgtga ccttcgtggg
     6181 cctggccccg ccgccggggc gcaccagcgt gaagtagtgg acggcgagac acgcgagggc
     6241 gaagcaggcg aggaagacga agccccacgc gtagccgccc gtcgcgtcgt ggatgaggcc
     6301 gagggcgagc ggcgggaaga agccgcccaa gcccccgaac gcaccgacca gtcccgtcac
     6361 agtccccgtc tctttcggga agaactgcgg gacgagcttg aagaccgcgc cgttacctaa
     6421 gccgagcgcg gcggcggcgc cgagcgcccc gaccgtgaag gtcaacatgt tggggcagcc
     6481 catcagcaga cctaagcctg cgaccgccgc gaagacgtag acgagcacgc ggacgccgcc
     6541 gatgcggtcg gccagccacc cgccgacggg ccgcatcagc gtcgccagca ggacgaagcc
     6601 cgcggtgcgc gcgcccgcgt cggtggcggt cagcccgaac aggtctttca acagcgtcgg
     6661 aagataaatg ccgagggcga cgaagccgcc gaaggtcaga aagtagaaga gcgagagcac
     6721 ccacgccatc ggctcgcgcc gcagcaccga gaggttttcg gaaagcgtct tcggcttggc
     6781 cgtcgtcgcc gcgtcgcgcg cgaagaggta gaagacgacg ccccagagaa acgccagcgc
     6841 ggcgaaggcg aagaagacgg gccgccagtc gccgccgaac gcgtaagcgg cgagcaccgg
     6901 cgcgccgaag acggccaccg actggccgat gttgcccatg ccgtagacgc cgagcgccgt
     6961 cccctgcttc tccgccgcga accatttcga ggtgaagccg acgccgacgg ggaaggtcgt
     7021 gcccgcgaga cccagaaaga ggccgagcag gaggagctgc gtgtagctcg tcgagaaccc
     7081 gatggcgacg gcgggcagcg ccgagaggat cagcagcgcg gcgtagacgc ggcgaccgcc
     7141 gaagcggtcg gcgagcatcc ccgccggcag ccgtccgatc gagccgagca ggacggggac
     7201 ggcaatcagc agactcttac tcgtcgccga gagctgataa atttgcgtga acgtcggcgc
     7261 gagcgccgcg atgagacccc acacggcgaa agagaccgcg aaagccgtcg tcgaaaagaa
     7321 gagcgcgcgg gccgcgccgg gtctgaaagt ttcccccatc atcattccct cacgtgttta
     7381 ccttgagcgg ggacaagctc cgcctcactt ccgaatgcaa cgggcgtgcc tctcaaaaca
     7441 ctgggaaacg ggtgactctt atcgtgggag gtgaggatta cttcgcaacc gcgggaaggt
     7501 gtcggggatt tttacgaagc gccgggcctc gcctttaacg cgagactacg aagtcagacg
     7561 gccccttcgg cgtcctcttc gtcgcaccgc ttacagtcga gttcgtagcc gaggcgcgag
     7621 gcgcggccct cttcggtcag cacccattcg cgcacgacga gcggcgggtc gtggcgcacg
     7681 tgctgcgggt ggccgcagtc gaggatggcg cgccagtggc cctcgtcgtc gtggtcgtaa
     7741 gccgtgatgc gccgcttcat ctgcccccct cgcggatgta gccggtgctc gtgtggcggc
     7801 gcgaggcggg ccgccaccag aggatgatgg tgagcgagat gagccagcag acgcccgacg
     7861 cgatgacgag gggcagcaac tcgccgtagc tgtgcgctaa ggccgcctcg accgtcgcgg
     7921 cgagaatcca gagctgcgcg acgttgatca tcacgagcat cacgagccgc gcctgctcga
     7981 ccgccgtccg cccgcgcccc gtccgcctgc gacccgcggg ggcttcaccg acaggataaa
     8041 cagtctgggt cgcgcgcgcc ctctgccgcg cttgcgcgtc ttcgcccgag ttaaaccgaa
     8101 cgctcatgtg cgcccgcctt tctcccgacg gcccagacct ccgcgccgcg ccgctccaga
     8161 actatctcgt cgagcgagcg cggcggcggg ccgtagagga cgttgcctgt ccgcacgtcg
     8221 aagccgccct cgtggcaagg gcattcgagc cgctggcgcc ggcgctcgta gtagaccggg
     8281 caggccaggt gcgtgcactt ctgcccgtag gcgtggtact cgccgtcttg cgtgcggacg
     8341 aggatggccg tgtcgtcctc ggtcgggtag cggaagttga gcgccgcgcc gggcgcgagg
     8401 gcttcggcac cctcgacctt cgcgggcgcg aaactctccg tcgggcgcga gtcgagcgcg
     8461 gccttgcccg cgaaacccgc ggcgctggcg aagagcgccg tcgaggtgag cgcgaggaag
     8521 ttgcagaact cgcggcgcgt gacgtgcgcc tcctcacctc gctcgtaagg gaagtcgcgc
     8581 tggtgcggcg cgacggcgcg gcgcacgtca ctttcatcgg aatgggcgtc agagcgtggg
     8641 ctgtcggcaa tgccgaagct cgtgaactcg ttgctggcgt tttcagaact cattgctttt
     8701 gtccgactcc tccacctgtg tgtctatcca cgactccccg gtcttgctct gctgctcggt
     8761 cagcccgagc cgcccctcga agacgccgca gccgtccacg cgcacctcgt cggcgtcggt
     8821 cgggagcatc aggtaaactc gcgtctcgac gcgctggttg ccgaagacgt ggacgttgac
     8881 cggcttcgtg cggcgcagcg gcacgatctg ttcgtacttg ccgaaggaga gcgcgcccgt
     8941 cgggcacacg ctcgcgcaca tcggcttcag cccttcgctc gtacggtcgt agcacatgtc
     9001 gcacttcatc tggaggtgca tctccgtgtt gtacttcggc acgccgaacg ggcacgcgtt
     9061 gacgcagttg cggcagtcga gacagcgcgg cttgagggcc gacatgacga tgccctcttc
     9121 gctcatcttg atggcgtccg ccgggcagac gagcgcgcag gtcggctccg tgcaatgcat
     9181 gcagaccgtg ggcatcgtcg ccgtcgtctc cgagcggtcg aggtagtcca cgaagatcat
     9241 cgagtagccc ttgtgcgtcc cgcactcgcg gcacgcggcg acgcacgagc ggcagccgat
     9301 gcaccgctgc gggtctatga acatcgtctc ttccataaga agctgttagc tattagctgt
     9361 tagccgttag ctcttcccct ttcgcaaaaa gaccgactgc gccggaagtg ttcaagtctc
     9421 gttgacactc aaaaagctga aagctgatag ctaacagcta acggctatct ccgagccgcg
     9481 tcaccgagct tcgctcggcg cctttgcgct gcttacccag aataggcaac tcgcgggtgt
     9541 gaatcttctc gacgcgcgcg gcgcacgcct tgtattcggg tatcttcgac gtggggtcga
     9601 gcgccgcgtt cgtcaactgg ttggccgcga gctgctcgcc ccagtggtag gggatgaaga
     9661 tggtgtcggg ccggatggtg cgcacgacca gcacggggaa gaccccctcg ccgcgacgcg
     9721 agacgacctt gacgcgctcg ccgtccgaga ctttcagcct tgcagccgtc tcggggtgaa
     9781 tctcgacgta aggctcggga cattgctgga cgaggaagcc gatgcgccgc gtctggttgc
     9841 cggagaggta ttgatagacg acgcggcccg aggtcaggat gagcgggtac tcttcgtccg
     9901 gcacttccgc cggcggtttg tactcgacgg cgtggaactt cgccctgccg tccgggtggt
     9961 ggaagcgttc ttcgaagagg cgcggcgtgc cggggtggtc ttccgcgggg cacggccaga
    10021 agacgccgtt ctgccgctcg atcttctcgt aggtgatgcc gtagtagtcc gcgttgccgc
    10081 cgcgcgaggc gacgcgtagc tcctcgaaaa tctcgcgcgg gctattgaac gggaaatact
    10141 tgccgcgccc catccggcgg gcaatctcct gcacgatcca ccagtcaacg cgcgcctcgc
    10201 cgggcgggtc ggcggccttg ttgattttga cgacgcgccc ctcgctgttg gccgtcacgc
    10261 cctcgtcctc ggcccaagtc gtgcccggca aaaccacgtc ggcgtagcgc gcaccctcgg
    10321 acatgaagaa gtctatgcaa acgtagaagt ccaagccttc gagcgagcgg cgcacctcgt
    10381 tgatgtcggg caagctcacc atcacgttgt tgcagatcga gagcagcccg cgtatctcgc
    10441 cctcgcgcat cttgtggaac atctcgacgc acgagacgcc cgcctgcggc agttcctctt
    10501 cgggcaagcc ccagaccccg cagacgaatt tgcggtgctc ggggttcatg atcgagcgct
    10561 gaccgggaag ctggtcggcc ttctgcccgt gctcgcgccc gccctgcccg ttgccctgtc
    10621 ccgtgatggt gccgtagccg cggcccggcg cgccgatctt gcccgtggcg aggacgaggt
    10681 tgatgtagga gaggacgttg ttgacgccgt tcgtgtgatg ctcgatgccg cgcgcgtgca
    10741 tcaccatgcc ggtctcggcg cgtccgtagg tgagcgcggc ctgcactatc ttcgcggcgg
    10801 gcacgccggt gatttccgac gcgacctgcg gggtgtacct gagcgccagc tcgcgcgtct
    10861 cttcccagcc gttcgtgcgc gcggcgatga agctctcgtc gatcagcttc tctcggacga
    10921 ggacgtggag catcccgttc gagagcgcaa catcggtgcc gggccgcagt tggaggtgga
    10981 tgtcgccctg ccgcgcggtc ggcgtctcgc gcgggtcaac gacgatccag acgccgccgt
    11041 tgtctctctg ctgccagagg aatttgttga ggacggggaa ggtttcggcg gcgttcgcgc
    11101 ccgcgaggat caagacctcg gcgagcggga tgtcgtccca cgggttggcc gcgcggtcga
    11161 tgccgaacgc cttgttgttg cccgccgcag ccgagaccat gcagagccgc ccgttgtagt
    11221 ccacgtagcg cgtgccaagc cccgcacgcg cgaacttacc catcagatat gtcttctcgg
    11281 tggtgaggga cgacccggaa tagacgccga ttgcatcctt gccgtgcttc gactgaagct
    11341 ccttgaactt cgagacgacg agatcgagcg cctcgtccca agacgcgcgc tcgaacatgc
    11401 cgtcgcgctt gacgagcggg tagagcagcc ggtcggggtg atgaacttgc aggtaggcgg
    11461 tgacgccctt cgggcagagg cggccctcgt tgacggggaa gtcgtaacgc ggctcgaagc
    11521 cgacgacgtg gttcttctcg accagcaggt tcatcccgca ctgcacggcg cagtaggggc
    11581 agtgggtggg gacgaccttg tcgatcacgc ggtcggcgcg ccagcccccg tgcggctcgt
    11641 cgtggaggtg cggcccgaac tgctcgacga tcttctctag gtttcctact ttgcccatcg
    11701 gcgactccga ggaatgtgtt ggtaggggca gggcttgtcc ctgcccgcac gtgaccgact
    11761 gattcaacta gaagttacgc agttgaagaa aatgtagtag aaaaagggcg tgaacccgcc
    11821 caagtgctcc gacgacgact acatcaactt cgtgatcgct acgccgcgct ctgtcagcgc
    11881 gaccgaagcg gcgaaagtac aaggcgatga ggagcaagcg ccggcccacg acgcctttac
    11941 gcgcttgctg catcggctgg aacctgacgc cgcgaccctg tggggtgaag cccggacgca
    12001 agtaaccttg acgcaaggca tccttgtcat agacgactcg accttggaca agccttacgc
    12061 ccgcaagatg gaactcgtca cgcgacactg gtcgggcaag caccacgcgg tggtgagtgg
    12121 catcaacctg attacgctgt tgtggacgga tggcgaccga cacgtccccg ttgactaccg
    12181 tatctttgac aagaaacagg acgctctgac caagaacgat cactttcagg cgatgctcag
    12241 agaggcgcac cagagaggct ttgcgccttc ctgcgtagcg ttcgatagct ggtactccgg
    12301 gcttgagaac ctgaagctga ttcgctcttt cggctggatt tggctgacgc gcctgaagag
    12361 caaccgcaag gtgaaccccg accgcgaggg cttgcgccct gtcaacgagg tcgagatcgg
    12421 cgcagccggg cgcgttgttt ggctcgaagg ctttgggctg attcgcgtgt tcaagatcgt
    12481 cgccacggac ggcgacgttg aactgtgggc caccaaccgt gtcgagatga gcgaccttga
    12541 gcgcgtccgc tgggcaggct actcgtgggc gattgagagt taccaccgcg gaatcaagca
    12601 gttctgcctg atcgagcggg cgcaggtgcg ctcgcgccgc gcgtggcgta atcacatcaa
    12661 cctgtgcttg cgagccttct tgcgactgga gagtcattgc tattacaaag gcatcagttg
    12721 gtatgaggcg aagacttcga tcatcagaga ggcggtgcgc gcctacatcg ccaatccgct
    12781 ctactcgctc gtctcaactg cgtaacttct atcaacgttg aattaaatga atcttgaaag
    12841 ggcagggaca agccctgccc ttacagtcat tgttcgcccc cctcacccct cgtcccttga
    12901 aaaacgcgct cgccgccgcc gggcaggttg acgtacgaga ggccgcgcat gacacgcttg
    12961 cagcgcgggc agaagtcctg aagcgtgcgc ccgtccggca tcgagtagtc gaagccgagc
    13021 gtggcgacca cctccttcaa atcgtcgagc cacatctgcc cgacgaactc cgtgccgcag
    13081 cgggggcaag tggcctgcgg cgcttcgcgc tcgcgccgct ggtaaagctc gacgccgacc
    13141 gaggccgggc gctggacgat gtggaagaac ttgccgaacg gcaggtagaa gagcgtcatg
    13201 atgacgagcg cctgatgcga gagggcgatg aagtagtaca tgtagccttc gagccagagc
    13261 gaggaggccg tcagcatcag ccccgtcacg gagaccgcga tgagcagcat gtgcggaacg
    13321 aagtcgagca tgaactgctg gagcgcgatg gccccgcggt cgttcaaacg ccggtggatg
    13381 gcgatggcgc agcccgcgat gaccatgacg gcggtgaagt cgagcgcgtg gaacgtgacc
    13441 cacgcgatga ggctgcggcc ctgcatgaca ttcggcacgg ggaagccgaa gaggtatgcg
    13501 cgatatcctt cgtcgccttc gagcttgaag tgcacccagc cgaagacgag cgggaaggtg
    13561 atgagcgcag agatgatgca gccccacatg atgagcgcgt gcatcagcca gcgcgtgaac
    13621 ccgcgccggg cgatgaagcg ctgctcgacg atgttgtgcg cgacggtctc gaaggcgctc
    13681 ccggcgtttt tagcgaggcg tttgcgttcg cggaagagct gccagccgcg ccgccagtag
    13741 acgcgcgtcg cgggcctgcg gagccagacg gcatagcggt agacgagcgc gccgaaggcg
    13801 accacggacg ccaccgtgta accgaagagc gccgggtcgt agtgttcgag gttgcgcgag
    13861 ccgacgacga ccaagaccgt cagcgcagcc gacgccgcca gcccgagcag gacggggcga
    13921 cgattcgtag tttccgccac cggcatttcg tggaaactcc tcctgatcct gtcaagacgc
    13981 gaaggttcga cggcgtatcg gcggtcgtgg gcgagggtat atacccgttc acgcacgacg
    14041 gccccggacg acggcacgcc gggacagtgc gctccacgac aacgagcagg gtagggtgca
    14101 cgcattatag accagcacgc ctcaacagcg aaagcttagt ccaaaaatga atgaaccggg
    14161 tgtgacataa gtcatctcgc gcgcggcccg accatgccat catgtgcccg ccgtggtgct
    14221 cttggggagt ccggcgaggg aggtagggat catgaaaagg gttgtcatcg aagaggtcga
    14281 agaatacgag gacgaaaagt tcgtccgtcc cgtgctgttc aaaggtgagc ggacgaccgc
    14341 gctgctgctc tgcctgaagc cggggcagtc cgtgcctccg caccgccacg aggggttcga
    14401 gatcatgctc cagccgctca ggggcgaggc cgaggccctg ctcgacgggg agcctgtgac
    14461 cctgcgcgcg ggcgaggtgc tcttcgtgga cggggcgaac gacttcgcgc ccgtgaacag
    14521 gggcggcgag aacttctcga tgctcatcac gctcgtgcgc cgctgaccgg ggcggcgcac
    14581 aacggctctc gcccgcaggg tagggtaatc cttgggcgag acggggcggc gcgcaccggt
    14641 cgtcggtgcg cgccgcccta aacgaagtcg ggcagtgccc gagccgaccg gcctcgaata
    14701 gccggtcggt actatggccg accgaggccg attaatgtag aggagtacga aagagatggg
    14761 gagcgttatg acagcgacac accccggcgg gggtctcaag accgacaaga tgcccggcca
    14821 ctgggttttg gcccgtctgg gcaagcgcgt gctgcggccc ggcgggatgg agttgacgcg
    14881 ccggatgctc gcctctctgt gcatcggcgg gtcggacgac gtggtcgagt tcgcgcccgg
    14941 catgggcgtc acggcccgcc tcacgctcgc ttcaagcccg gcgtcctaca cggcggtcga
    15001 gcgcgacgag gccgccgcgc gcatcgtcaa aggctacgtc gtcggcgaga gccggcgcgt
    15061 gatcgtcggg agcgcggacg agacgggtct tccagcggcg tcggccacgg tcgtctacgg
    15121 tgaggccatg ctgacgatgc agaccgacga gacgaagcgg cggatcgtgc gcgaggcggc
    15181 gcggctgctc cggcccggcg ggcgctacgc catccacgag atgtgcctcc gcgacggcgt
    15241 gggcggggaa gtgcgcgaag aggtggagcg tgcgctcacg ggcgtcgtcc accacggcgt
    15301 cagaccgctg acggtcactg agtggcggcg tctgctcgaa agcgaaggcc tcgaggtgaa
    15361 ggccgtcgag accgcgccga tggccctgct cgaaccggcg cgcgtcgtcc gcgacgaggg
    15421 gttcttcagg gcgctgcgct tcgccttcaa cgtgctgcgt gacggggacg cgcgccggag
    15481 ggtcttcggg atgaggagag ttttccgccg ccaccgcggg cagctcgcag ccgtggcggt
    15541 gacggcggtg cgcgcgtgaa cgcgggccgg gcggcctttg aaccgggcga ggtgtgactt
    15601 ggtttagcgc gtcgcccctt ccgagaaacg cgggggaagg ttctcgatct cgtgccgcac
    15661 gccgtcgcgg agcgtgtcga gttctgctcc ggcgagaagc gcgtcgggcc agaggtagac
    15721 ttgctgctcc tccacgcggt tgtgctcgac aagccgctcg gctacagccg aaactcgccc
    15781 cctgatttgc tgaagccgtt cgagttcagg cgggccgtcc tgcgcggcca tctcgcgcgc
    15841 ggcgttcacg gccccggcca gctcgcgcat gaagaagtcg tggtcttcgc gcaggcgctc
    15901 gacggccaca cgcacctccc ccggcgtcgg cgcgtcgtcc gcctccacgc gtcgcctcgt
    15961 ctcggaggcc gcgagcagag cggggaagag gtgcaggtgc tcggccctga tgtgaacggc
    16021 gagccgcgcc cacaggtagt ccagtttctc gaacactccg cgcgcttcgc cgccgtcgaa
    16081 ctcaggccac aggtcacgga acagggtatc gacctcggcg tgatcgtcta ataaaaggtc
    16141 tgtcatctta tcgcctcctt ccgctgcgcg ggtcaggcgg taagagcgta gcgaaactcc
    16201 gccgcgcgtg gcaaacagtc cgggggccgc gcacgctttt cgaactccac ggcccggaga
    16261 cgcttgaggt gcgtgatgca cagtatgcgg cccgtcggct tcgtcagaac caccttcacc
    16321 gagaactcgc agatcccgaa agggcgcggg gccaaacacg aggccgaagg cttcatcgag
    16381 atcgagcctg agttcgagga ggggctgttg gacatcgagg gcttctcgca tctctacgtc
    16441 atctgggtct tccacgcgtc cgagggctat gaactcacag gcgtcccgcc gaccgacgac
    16501 cgcccgcacg gcgtcttcgc cacgcgctcg ccctaccgac cgaacccgct ggggctgacc
    16561 gtagtgcggc tgctcgcgcg ggagggccgc acccttcgcg tcggcggcgt ggacctgctc
    16621 gacgggacgc ccgtcctcga cctcaagccg tacctgtcaa acgtacccga ggacgaactg
    16681 cggcgcggct ggatggatga tgcgtctcaa gtatagtgtt tcataagttg cttctcgcgt
    16741 gtcaatacgc tcttttctat catcacacgc ctgactgaat tctgcctctt gagacgtgat
    16801 ttctcatcat aagtgctcgt tcaaaccgat gggcggcact atacccctca gcgtcaagcc
    16861 ggaatccggc ttggctttgc accccacctg tccctgattc ggaaggaaga tgtgcatagc
    16921 gcgtcgtgcg caaaattgcg cgcgcggtgg aaatctacgc acgcgaccgg ccggcggcct
    16981 ccgaaaccaa gcgaaagtgc gcgcgacgga cgacgaaacg gtggcacggg tgatgctctg
    17041 acaagacggc aaggcccatc accgtcggca ggcgtcaact gctgaaggag ggggcatggc
    17101 gttcaaagga agacggccca ggaggaggtg ttcggcatga agatattact tgcggttgac
    17161 ggctcggtct tcggcgaaga ggcggccaga gaggtggcgc gccgcccgtg gccggagggc
    17221 agcgaggtca aagttatttc cgcggtcgaa cccgtttacg tgccggcggc ggagccgtgg
    17281 gggctgcccg caggctattt cgttaacctg gacaagctgg cggacgatag agcgcgttcc
    17341 accgtcgagg ccgcgctcag gttactgagt gaccgcgaag gcgccccgct gaaggtctcc
    17401 acggcgaggc cctgcggccc ggcccggcag gtgatcgtgg aggaagccga gcggtggggg
    17461 gccgacctcg tcgttctcgg ctcgcacggc tacgggttct ggaatcggct gctgctcggc
    17521 tcagtgtcgc aggcggtggc ctcgcacgcc ccctgcacgg tcgagatcgt gaagcggccc
    17581 cggcccgcag aaggtggggt gaaagatggc taacgtgctc gtcctcggcg gcgggttcgg
    17641 gggcgtcgtg gccgccgagc ggctggcgaa gctgctcgtg ccggagcacc ggatcacact
    17701 ggtgtcgcgc tcgcggcggt tcgtcttcta cccggcgctg gtgcggctcg ccttcggcgg
    17761 gtgcgagccg gacgacatca cgctcgacgt gcgcgaggct ttgaccgaca ggcgcgtccg
    17821 gttcatcgag gccgaggtgg cgcgcgtcga gccgctggaa cgcaaggtgg tgctcgcgca
    17881 cggcgaggtc gcgggcgagc tgggctacga ctacctcgtc ttcgcgctcg gcaggcgtct
    17941 cgccaccgag cgcgtgaggg gcttcttcga gcacgcgcac cacctgttga cggtggaggc
    18001 ggcacttaaa ttcggcgagg cggtgcgagg tttcgacgaa gggcacgcgg tcgtcggcta
    18061 ctgcgaaggc gcgcggctcg ccctgcccgt ctacgagacg gccatcgcgc tctcgcgcgc
    18121 cctcgaagcg cgcggcgtgc gcgggcgggc gaagataacg gtagtcacgc cggagctgcc
    18181 gggcgagcgc atggagggcg acgagatgcg gcgaccgctg cgcgaggcgc tcgacgcgca
    18241 cggcatcgac ttctggccca acttccccgt caaccgcgtg accgcggagc gcgtacacgc
    18301 ggacggcggg ccgggcatgc gctacgacct cctgatgctg ctgccgccgt tcgcctgttc
    18361 gagcacggtc atggatcagg gcatcacgga cgtcgaaggg tacgtccgcg tcgcgcagtc
    18421 gatgcgcgtg gcgagcgtcg agcggatgta cgccgtgggc gattgcgtcg gcctgcccgg
    18481 cccgaagatg gcgcacatgg cggtcaacca ggccgaggtc gccgcccgga acctcgccgc
    18541 ggagatcgaa gggcgcacac ccgaagccgt ctacaggcac gagatcaagc tggtcatgga
    18601 agaaggcggc gacgactcga tatacttcca caaagattta gagggcgagg gcgcggcgag
    18661 cgtgcggcgc gggcggttct ggggctgggc taaacgggcg cacgaaaagt attggcaagc
    18721 ccgacacggt taacgtccgg acgtaggagc aggttgtctc ataaaacatg aggttcgttt
    18781 ctcgccgtcg aggcgacgtt gaccagagga gtatcatgga cggaaagatg aagatactgt
    18841 tcgcctacga cggttcggag tgcgccgacg cggcgctcga agacctacgg cgggcggggc
    18901 tgcccgccga agccgaggcg ttcgtgctct cggtcgccga tgtcatcctg ccgccgccgt
    18961 tcgacgaagt agccgacgaa gccttgcctc ttcacctggc cttgggcatc aggcgcgcgc
    19021 acgagcacgc ggcgcagacc gtcgagcggg cgcgcggtct ggcggagtac gccgcggggc
    19081 gcgtcagaac atccttcccc ggatgggagg tgcgggccga ggcgtgggcg agttcgccgg
    19141 cgtgggcggt cgtcatgaag gcagacgcgt ggaagcccga cctcgtcgtg gtcggcgcgc
    19201 acggccacac ggtcatcggc gggcggctga tcctcgggag cgtgtcgcag agggtgttgt
    19261 acgaggcgcg ctgctcggtg cgcgtcgcgc gcaactcgca caggcccgac ggctctcccg
    19321 tccgcgttct cgtcggtacg gacaactcgc cggacgcggc ggcggcgatt gacgcggtgg
    19381 ccgcgcgcgc gtggcccgcg gggacggagg ttcgcctcgt cgccgtgctc gacacggtca
    19441 tgccggtgtc gcagagcgag ggcggggttc acgtgctcag gtgggtcgag gtcgggaaag
    19501 aggaagagtg gggctgggtg aaagacatct tcgaagcgtc cgccgaaagg ctgcgcgcga
    19561 agggtctcga caccacggtt gtcctcaaga aggggaaccc caagcacgcc ctcatcgagg
    19621 aagccgaaag ctggggcgcg gactctatct tcgtcggcgc gagaggcgcg cgcggaattg
    19681 acaggctcct gatcggcagc gtctcggcgg cggcggcggc gcgcgcacac tgctcggtcg
    19741 aagtagtgcg cccgccggag aagccgccgg aagccggggc gtgaaggaca gcctgcaaga
    19801 cgtggccgcc tccccgatta tgaaagaaga gcctgaaagc cgacttcccg aaggtcgatg
    19861 ttcctgttga gagcatcggc ggtgtggctg gtcttgatga tcgccgaatc cgtccacggc
    19921 gttctcagga tgatgcttat cgcgccccgc gtcggggatt tcaaggcccg gcagatttcg
    19981 gtcttcaccg gctcgcttct gatactggcc gtggcctatc tgttcgtccg ctggattcgg
    20041 gccgggacga cgggaaggct gctcgcagtg ggcctcgtct ggctcgccct caccgtgctg
    20101 ttcgaggtgg cgctcgggcg cttcgtgatg ggttattcgt gggacagact ggcgtcggat
    20161 tacgacctcg cgcgcggcgg gctgatgcct ttcggcctcg tcatcctggc gctctcgccc
    20221 ctcttcgcgg cgaagtcgag agggcttggg aagggcgtca gggcgtctca cgtcgaagga
    20281 gagccggcgg cgcggcggac ttgaatccga cgggcggtca cggcgcaacc gacatggctg
    20341 acttgaacca gacggcggag cacgggcact cgcgtgagga catccgcgag aggctggcgg
    20401 cggggccgcg ccacaactac ctgcgcgact ggatctacgg cgggatcgac ggcgccgtca
    20461 cgaccttcgc cgtcgtcaca ggggtcgtcg gggccgacct atcgccctcg atcatcctcg
    20521 tgatgggctt cgccaacctc gccgcggacg gcttctcgat ggccgcctcg aactacctcg
    20581 ggacgaaggc cgagcaggag gacttcaagc accttgaggc ggtcgagcgg cgccacatcg
    20641 agacggtgcc ggagggcgag cgcgaggagg
//
LOCUS       DBNGOH010000329         2073 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2616356, whole genome shotgun sequence.
ACCESSION   DBNGOH010000329 DBNGOH010000000
VERSION     DBNGOH010000329.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2073)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2073)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2073
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2616356"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="AC159P_06375"
     CDS             complement(<1..234)
                     /locus_tag="AC159P_06375"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HIR3695117.1"
                     /translation="MSIVYEERDALAFVRLARAARGNALTGAMLARLGEIFEGVARRR
                     DLRAVILSGEGENFCAGTDGAEPAAFDAAGARLR"
     gene            complement(354..1244)
                     /locus_tag="AC159P_06380"
     CDS             complement(354..1244)
                     /locus_tag="AC159P_06380"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003857 - (3S)-3-hydroxyacyl-CoA
                     dehydrogenase (NAD+) activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HIR3695118.1"
                     /translation="MEIKKVGVLGCGLMGSGIAQTAATAGLDVVVREVTQELCDRGFV
                     GIDKSLAKFAEKGTITPDEQAGIRGRMRGTTALEDLADCDIIIEAIIENLDTKRETYQ
                     RLDELCKPETIFASNTSSLSITEMMTATSAERQQRFVGLHFFNPVPLMKLVEVVRTIL
                     TDEAVYETAVEFGKRLGKVPVRAGDRSGFIVNRLLVPYLLDSIRALEEGVGSIVDIDN
                     GMKLGCGHPMGPLTLLDFVGLDTTYYIAEIMFNEFRERRFAPPPLLKRMVLAGLYGRK
                     SGRGFYDYADPKNPTPMNLL"
     gene            complement(1310..>2073)
                     /locus_tag="AC159P_06385"
     CDS             complement(1310..>2073)
                     /locus_tag="AC159P_06385"
                     /EC_number="3.4.21.-"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HIR3695119.1"
                     /translation="DYPAAGASGALFGLVGVLFVFGIKYRKELPEDFKRAFGFGMLPM
                     ILLNLLIGYLARGVVDNAAHLGGLLAGVVLALFVGYKRPGEPTRRAALWRLAQVALLA
                     LVAASFAAAWRDFGGPRPDSTRFSLRGLFSLAGPSRYLGAINESETAFHTALNGDPEP
                     AERVVKELDEAPPLDDREKLLRGELKALLLSAQQFGSTDPEERKKADALRQREQLVTT
                     YDEWNKRFLEWVKGEGGNYGILIREGEEAAPEGKN"
BASE COUNT          326 a          691 c          700 g          356 t
ORIGIN      
        1 ccgcagacgc gcgcccgccg catcgaacgc cgccggctcc gcgccgtccg tgcccgcgca
       61 aaagttctcg ccctcgccgc tcaggatgac ggcgcgcagg tctcgccgcc gcgcgacgcc
      121 ctcgaagatt tcgccgagcc gggccagcat cgctcccgtc aacgcgttgc cccgcgcggc
      181 ccgcgcgagc cgcacgaacg ccaacgcgtc acgctcttcg taaacgatgg acatacaaaa
      241 atggtgaatg gtgaatggtg aatggttatg agttgttcgg cccgcgatat accgctcagg
      301 ttgaagcacc gctcaaccac cattcaccat tcaccattta ctattcaccg ccttcagagc
      361 agattcatcg gcgtggggtt cttggggtct gcgtagtcgt agaagccgcg gcccgatttg
      421 cgaccgtaga ggccggccag aaccatgcgc ttgaggagcg ggggcggggc gaagcgccgc
      481 tcgcggaact cgttgaacat gatctcggcg atgtagtagg tggtgtcgag gccgacgaag
      541 tcgagcagcg tgagcggccc catcgggtgg ccgcagccga gcttcatgcc gttgtcgatg
      601 tccacgatac tgccgacgcc ctcttcgagc gcgcggatcg agtcgagcag gtagggcacg
      661 agcaggcggt tgacgatgaa gcccgagcgg tcgccggcgc gcacgggcac cttgccgagc
      721 cgcttgccga actcgaccgc cgtctcgtag accgcctcgt ccgtgaggat ggtgcggacg
      781 acctcgacga gcttcatcag cgggacgggg ttgaagaagt ggaggccgac gaagcgctgc
      841 tgccgttcgg cggaggtggc cgtcatcatc tcggtgatcg agagcgagga ggtgttcgag
      901 gcgaagatgg tctcgggctt gcagagttcg tccagccgct ggtaggtctc gcgcttggtg
      961 tcgaggtttt cgatgatggc ctcgatgatg atgtcgcagt cggcgaggtc ttcgagcgcg
     1021 gtcgtccccc gcatccgccc gcgaatcccg gcctgctcgt cgggcgtgat cgtgcccttc
     1081 tcggcgaact tcgccagaga cttgtcgatg cccacgaagc cgcggtcgca caactcctgc
     1141 gtgacctcgc gcaccaccac gtcgaggccg gcggtggccg ccgtctgcgc gatgcccgaa
     1201 cccatcaggc cgcagccgag caccccaact ttcttgatct ccatacgttt tcggattctc
     1261 ctgtcttgaa tttttgcggg cggacggtgt gcgccgcccg aagggacggc taattctttc
     1321 cctcgggcgc ggcttcctcg ccctctctga tgaggatgcc gtagttgccg ccttcgccct
     1381 tgacccactc aaggaaacgc ttgttccact cgtcgtacgt cgttacgagt tgttcgcgct
     1441 ggcgcagcgc gtccgccttc ttccgctctt cggggtcggt cgagccgaac tgctgcgcgc
     1501 tgaggagcag cgccttcagc tcgccgcgca gcagcttctc gcggtcgtcg agcggcggcg
     1561 cctcgtccag ctccttgacg acgcgctcgg ccggctccgg gtcgccgttg agggcggtgt
     1621 ggaaagcggt ctcgctctcg ttgatcgcgc cgaggtagcg ggacggcccc gcgaggctga
     1681 acagcccgcg caggctgaag cgggtcgagt ccggtcgggg gccgccgaag tcgcgccacg
     1741 cggcggcgaa gctcgccgcg acgagcgcga gcagcgcgac ctgcgcgagg cgccacaagg
     1801 cggcgcggcg cgtcggctcg cccggtcgct tgtagccgac gaagagcgcg agcacgacgc
     1861 ccgccagcag cccgccgagg tgcgccgcgt tgtccacgac gccgcgcgcg aggtagccga
     1921 tcagcaggtt gagcaggatc atcggcagca tgccgaagcc gaaggcccgc ttgaagtcct
     1981 cgggcagctc cttccggtac ttgatgccga agacgaagag cacgccgacc agtccgaaga
     2041 gcgcgcccga ggcgcccgcc gccgggtagt cgc
//
LOCUS       DBNGOH010000330         6020 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_783519, whole genome shotgun sequence.
ACCESSION   DBNGOH010000330 DBNGOH010000000
VERSION     DBNGOH010000330.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6020)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6020)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6020
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_783519"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="AC159P_06390"
     CDS             <1..336
                     /locus_tag="AC159P_06390"
                     /inference="COORDINATES: protein motif:HMM:TIGR02241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098015 - virus tail [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HIR3695120.1"
                     /translation="VAEYRNGNDPENHVRKINGVYKVPDVTKKRGLIGDLDFFGWLKD
                     IREGAQDNVLEQVTIELMAEDHTGPVMTWRLKNARPIKYTGPTLNAKGGTDVAMEELV
                     LACESLSIE"
     gene            481..3228
                     /locus_tag="AC159P_06395"
     CDS             481..3228
                     /locus_tag="AC159P_06395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695121.1"
                     /translation="MIRASNIRLPGVYFLPSPRARGPRLPPLDVAAFVGFAERGPLDF
                     PVAVEDLDTYRAIFGGDLALAREAGGRTVYANLPTAVASFFAQGGRRCYVVRVAGREA
                     TRTLFPLRGLVSVRGPHDPASGETPQLASVWASSEGRWSANLRLGARLRSTPLPPDAF
                     GVLAPQAEVIDGVPRVTQRLAWTTGSAPEAIQPGDLLRLTYNNDRQYLFPVEEARRPA
                     DAASPPPPVELRTRYAWELLAHPHADTTRIVVRVDRVTPQGLERLNISGRFVSYARED
                     RPGFFFTRMTGPDAARIAVGDVLQIRRIDNIIGLFPVEEAGTLQEDEGLALPFARAAR
                     WLRLPAQQIPQTPPSTLEAVERLRFDLLVRAGNDRRPTVEELSFNHGHPRFWGDAVLL
                     GSSALGRQAEGGDLTRASNARPAVAGGRAPQLSHDAESAEIFTLLRGDARLEETRLMR
                     LDPAALGGLLAPAVEADRTFLPLAMLSILDEADIHGPAEETVGDDGLDAFDPSLFVDT
                     ALVRLDNQLSADALMAEAFDRYYVQDKRLRGLHSLLFTGEAALIALPEAVQRGWPGEV
                     EPDDAPLPEPSPAPEPPDLSKFIDCERAVTLSPHVLSPHVTSPPAAVATEPELPVLKP
                     IDTDDEDDPQYEAELANLTRLHRALLTFCQARRDAFGILALPHYFEKRHCIKWQETFR
                     ALLGMPARRTVFTDARDIADLSYVAVYHPWLLISDEAAADGLRAVPPDGAACGMIAAR
                     ERERQVWVAPAGVPLAGVLGLAPAIPTDDWADLFDLQFNLVRREPRDFRAMSAHTLSD
                     ESIWLQISVRRLLILIRKFVVARGLDFVFESNHERFRSGVRLMLEDALRYMYERGAFA
                     GDAPEQSFNVVTDASVNPPQSVDAGRFVAQIQVAPSQPLEFITVLLTRVGEDLLQARE
                     A"
     gene            3233..3724
                     /locus_tag="AC159P_06400"
     CDS             3233..3724
                     /locus_tag="AC159P_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014395682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098015 - virus tail [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HIR3695122.1"
                     /translation="MADEYPFTAFRFDVQLMFEDPGSIGVTQPLCGALFAECDGLEMT
                     MEPKTLREGGNNLEQIHLAGPVSYGQLSLKRGMTANLDLWKWFNAAAGGLNTGQSANR
                     RGLTAQGLVTMRDGAGKPTLLFDLAGCLPVKIKGSPLSGKDGQVALEEMQIAYRSLKV
                     KTP"
     gene            3741..4652
                     /locus_tag="AC159P_06405"
     CDS             3741..4652
                     /locus_tag="AC159P_06405"
                     /inference="COORDINATES: protein motif:HMM:NF039279.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CIS tube protein"
                     /protein_id="HIR3695123.1"
                     /translation="MALKKARLVEIKWDSNQAKAKPGDSKRTVEVHFNPQTLKVSFSN
                     KLTGGNQPGGSSKQATGSRETKLSIELLFDTTLDGTDVREITKKVSYFITGDDGTGGA
                     GGAAKHSPPGIQFEWGSFVFRGVTDAMDETLDYFSDDGVPLRATITLNITRQEIEFIA
                     GEPGQGGGGRQGDIGPIQPLATEPLAPANTGDSMQSMAGRNGNSGDWKSIAAANNIDD
                     PLRLQAGALIDLNAGVSVGARLGASAGLSANVGAGAGVSASALAKAGASAGVSARTVA
                     SANANAGASAGASVSGGAGASLRAGRR"
     gene            4681..4860
                     /locus_tag="AC159P_06410"
     CDS             4681..4860
                     /locus_tag="AC159P_06410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695124.1"
                     /translation="MPVIINDFDIMTDSTEPPPKRGSGDGPTTPQSQPPQLRPEDIKQ
                     VMRINRDRMERVRAD"
     gene            5027..>6020
                     /locus_tag="AC159P_06415"
     CDS             5027..>6020
                     /locus_tag="AC159P_06415"
                     /inference="COORDINATES: protein motif:HMM:NF017739.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
                     /protein_id="HIR3695125.1"
                     /translation="MPDNAPTTGFYAATPTLKVDGKEEAALGLLLESLLVEETTLGLF
                     RCEAHIGNWATKGDEPDFLYFDSQVIDFGKEFAVEFGPPGAQTEIFKGRITGLEAHYP
                     ETSTATLVILAEDRFQDLRMERRTRSFEDVSDADIIKRIASQHGLTPDVDVDGPTYRV
                     ITQTNQSDLAFLRERAAAADAELWVADRTLHAQARSRRKTNKVELSWKSNLLEFSVMA
                     DLAHQRTSVRVSGWDVSSKSALDEEAGVSVVNGELDGRRSGSAVLAAALAERHERVVM
                     TVPLSQPEAKAVAEARYRARARRFVRGRGQADGTPELRVGTSLTLGGLGDFFDGK"
BASE COUNT          987 a         2169 c         2050 g          814 t
ORIGIN      
        1 gtggccgagt accgcaacgg caatgacccc gagaaccacg tccgcaagat caacggcgtc
       61 tacaaggtgc cggacgtgac caagaagcgc ggcctcatcg gcgacctcga cttcttcggg
      121 tggctcaagg acatccgcga gggcgcgcag gacaacgtgc tcgaacaggt caccatcgag
      181 ctgatggccg aagaccacac cggcccggtg atgacgtggc ggctgaagaa cgcgcgcccc
      241 atcaagtaca ccggcccgac cctcaacgcg aagggcggaa cggacgtggc gatggaagag
      301 ctggtgctcg cctgcgagag cctcagcatc gagtagcgcg cgcgtgtcgt cccacgactg
      361 taggggcagg acttgtccct gcctgtgccg cgtgctcact cgacgttgaa tcaaccgaaa
      421 gttgatgggc agggacaaac cctgccccta cagtcttcat ccgcggcgag tatcttcggc
      481 atgatacgag cgtccaacat ccgcctgccg ggagtctact tcctgcccag cccgcgcgcg
      541 cgcggccccc gcctgccgcc gctcgacgtg gcggccttcg tcggcttcgc cgagcgcggg
      601 ccgctcgact tcccggtcgc ggtcgaagac ctcgacacct accgcgccat cttcggcggc
      661 gacctcgcgc tcgcgcgcga ggcgggcggg cgcaccgtct acgccaacct gccgacggcg
      721 gtcgcctcct tcttcgcgca gggcgggcgc cgctgctacg tcgtgcgcgt cgccgggcgc
      781 gaagcgacgc ggacgctctt ccccctgcgc ggcctcgtct ccgtcagagg cccgcacgac
      841 cccgcctccg gcgagacgcc gcagctcgcc tccgtctggg cctcgtccga ggggcgctgg
      901 tctgcgaacc tccggctcgg cgcgcgcctg cgcagcacgc cgttgccgcc ggacgccttc
      961 ggcgtgctcg cgccgcaggc cgaagtgatc gacggcgtgc cgcgcgtcac gcagcgcctc
     1021 gcgtggacga ccggcagcgc gcccgaggcc atccagccgg gcgacctgct gcgcctcact
     1081 tacaacaacg accgccagta tctcttcccc gtcgaagagg cgcggcgtcc cgccgacgcc
     1141 gcctcgccgc cgccgcccgt cgagctgcga acgcgctacg catgggaact gctcgcgcac
     1201 ccgcacgcgg acacgacgcg catcgtcgtc agggttgacc gcgtgacgcc gcagggactc
     1261 gaacggctca acatctccgg tcgtttcgtg tcctacgcgc gcgaagacag accgggcttc
     1321 ttcttcacgc ggatgaccgg ccccgacgcg gcgcgaatcg ccgtcggcga cgtgttgcag
     1381 atcaggcgca tcgacaacat catcggcctc ttccccgtcg aggaggcagg gacgttgcag
     1441 gaggacgagg ggctggcgct gccgttcgcg cgcgccgcgc gctggctgcg tctgcccgcg
     1501 cagcaaatcc cgcagacgcc gccctcgacg ctcgaagcgg tcgagcgcct gcgcttcgac
     1561 ctgctcgtgc gcgcgggcaa cgaccgccga ccgaccgtcg aggagctgtc cttcaaccac
     1621 ggccacccgc gcttctgggg cgacgcggtg ctgctcggat cgagcgccct cggcagacag
     1681 gccgagggcg gcgacctgac gcgcgcctcg aacgcgcgcc cggcggtcgc gggcgggcgc
     1741 gcgccgcagc tctcgcacga cgccgagagc gccgaaatct tcacgctcct gcgcggcgac
     1801 gcgcgtctcg aagagacgcg gctcatgcgg ctcgaccccg ccgcgctcgg cggcctgctg
     1861 gcgcccgccg tggaggccga ccgcaccttc ctgccgctcg ccatgctctc gattctggac
     1921 gaggccgaca tccacggccc cgccgaagag acggtcggcg acgacggact cgacgctttc
     1981 gacccgagcc tcttcgtcga tacggcgctc gtccggctcg acaaccagct ttcggcggac
     2041 gcgctgatgg ccgaagcctt cgaccgctac tacgtgcagg acaagcggct gcgcggcctc
     2101 cacagcctgc tcttcacggg cgaggcggcg ctcatcgcgc tgcccgaggc cgtgcagcgc
     2161 gggtggcccg gcgaggtcga gccggacgac gcgccgctgc cggagccgag ccccgcgccc
     2221 gagccgcccg acctctcgaa gttcatcgac tgcgaacgcg ccgtcactct ctcgccgcac
     2281 gtcctctcgc cgcacgtcac ttcgccgccc gccgccgtcg cgacagagcc ggagctgccc
     2341 gtgctcaaac ccatcgacac ggacgacgag gatgacccgc agtacgaggc ggagctggcg
     2401 aacctcaccc ggctgcaccg cgcgctcctg accttctgcc aggcgcggcg cgacgcgttc
     2461 ggcatcctcg cgctccccca ctacttcgag aagcgccact gcatcaagtg gcaggagacc
     2521 ttccgcgcgc tcttggggat gcccgcgcgc cgcacggtct tcaccgacgc gcgcgacatc
     2581 gcagacctct cctacgtcgc cgtctaccac ccgtggctgc tcatctcgga cgaggcggcg
     2641 gctgacggct tgcgcgcggt gccgcccgac ggcgcggcgt gcggcatgat cgccgcgcgc
     2701 gagcgcgagc ggcaggtgtg ggtcgcgccc gcgggcgtgc cgctggcggg cgtgctcggc
     2761 ctcgcgcccg cgattccgac cgacgactgg gccgacctct tcgacctcca gttcaacctc
     2821 gtccggcgcg agccgcgcga cttccgcgcg atgagcgcgc acaccttgag cgacgagagc
     2881 atctggcttc agatttccgt ccggcggctg ctcatcctca tccgcaagtt cgtcgtcgcg
     2941 cgcgggctgg acttcgtctt cgagagcaac cacgagcgct tccgctcggg ggtgcgcctg
     3001 atgctcgaag acgcactgcg ctacatgtac gagcgcggcg cgttcgcggg cgacgcgccc
     3061 gaacagtcct tcaacgtcgt cacggacgcg agcgtcaacc cgccgcagag cgtggacgcg
     3121 ggccgcttcg tcgcgcagat acaggtcgcg ccgtcgcagc ctctggagtt catcaccgtc
     3181 ctgctgacgc gcgtcggcga agacctgctg caagcgcggg aggcgtgaaa cgatggccga
     3241 cgaatacccc ttcaccgcct tccgcttcga cgtgcagttg atgttcgaag accccggcag
     3301 catcggcgtc acgcagccgc tctgcggcgc gctcttcgcc gagtgcgacg gcctcgagat
     3361 gacgatggag ccgaagaccc tgcgcgaggg cggcaacaac ctcgaacaga ttcatctggc
     3421 cggccccgtc tcctacggcc agctcagcct gaagcgcggg atgacggcga acctcgatct
     3481 gtggaagtgg ttcaacgccg ccgccggggg actcaacacg gggcagagcg cgaacaggcg
     3541 cgggctgacg gcgcagggcc tcgtgacgat gcgcgacggg gccgggaagc cgacgctgct
     3601 gttcgacctg gcgggctgtc tgccggtcaa gatcaagggg tcgccgctga gcggcaagga
     3661 cgggcaggtg gcgctcgaag agatgcagat cgcctaccgc tcgctgaagg tgaagacgcc
     3721 gtaaaggggg cacgaagcgc gtggcactga agaaagccag actcgtcgag atcaagtggg
     3781 actcgaacca ggcgaaggcc aagcccggcg actcgaagcg caccgtcgag gtgcacttca
     3841 acccgcagac gctgaaggtc tctttctcga acaagctgac gggcggcaac cagcccggcg
     3901 gctcttcgaa gcaggcgacg ggcagccgcg agaccaagct ctccatcgag ctgctgttcg
     3961 acacgacgct cgacggcacg gacgtgcgcg agatcacgaa gaaggtctcc tacttcatca
     4021 ccggcgacga cgggacgggc ggcgcgggcg gcgccgccaa gcactcgccg cccggcatcc
     4081 agttcgagtg gggcagcttc gtcttccgcg gcgtcacgga cgcgatggac gagacgctcg
     4141 actacttctc ggacgacggc gtgccgctgc gcgcgaccat cactttgaac atcacgcggc
     4201 aggagatcga gttcatcgcg ggcgagccgg ggcagggcgg cgggggccgc cagggagaca
     4261 tcggccccat ccagccgctc gccaccgagc cgctcgcgcc cgccaacacc ggcgacagca
     4321 tgcagagcat ggcgggccgc aacggcaaca gcggcgactg gaaatccatc gccgccgcca
     4381 acaacatcga cgacccgctg cgtctacagg ccggcgcgct catcgacctg aacgcgggcg
     4441 tcagcgtcgg cgcccgtctc ggcgcgagcg cggggctgag cgccaacgtc ggcgcgggcg
     4501 cgggcgtgag tgcgagcgcg ctggcgaagg ccggcgcgag cgcgggcgtg agcgcgcgca
     4561 ccgtggcgag cgccaacgcc aacgcgggcg cgagcgcggg cgcgagtgtg agcggcggcg
     4621 cgggcgcgag tctgcgggcg ggccgtcgct gaagcgacat gaatgtgggg tgaagcgaag
     4681 atgccggtca ttatcaacga cttcgacatc atgaccgatt cgaccgagcc gccgccgaag
     4741 cgcggttcag gcgacggccc gacgacgccg caatcacagc cgccgcagct ccgccccgaa
     4801 gacatcaagc aggtcatgcg catcaaccgc gaccggatgg agcgcgtgcg cgcggactga
     4861 acgcacgtcg caagactgta ggggcagggc gtgtccctgc ccctcaacag ccgattgatt
     4921 caacgtcggg tgaccatgca cctctgcggg cagggacaag cccagcccca acagtcatca
     4981 cgaacgctga agttaatagc cgggagtcgt aacgagcgaa cgagccatgc cggacaacgc
     5041 accgaccaca ggattctacg ccgcgacgcc cacgctgaag gtggacggca aggaggaggc
     5101 cgcgctcggc ctgctgctcg aatcgctgct ggtcgaagag acgacgctcg ggctgttccg
     5161 ctgcgaggcg cacatcggca actgggcgac gaaaggggac gagccggact tcctctactt
     5221 cgactctcag gtcatcgact tcggcaagga gttcgcggtc gagttcggcc cgcccggcgc
     5281 gcagaccgaa atcttcaagg ggcgcatcac gggcctcgaa gcccactacc cggagaccag
     5341 cacggcgacc ctcgtcatcc tcgccgaaga ccgcttccag gacttgcgga tggagcggcg
     5401 cacgcgctcg ttcgaggacg tgtcggacgc ggacatcatc aagcgcatcg cctcgcagca
     5461 cggactcacc ccggacgttg acgtggacgg gccgacctac cgcgtcatca cgcagaccaa
     5521 ccagagcgat ctggccttcc tgcgcgagcg cgccgccgcc gcggatgcag agctgtgggt
     5581 cgccgaccgc acgctccacg cgcaggcccg ctcgcgccgc aagacgaaca aggtcgagct
     5641 gtcgtggaag tcgaacctgc tggagttctc cgtcatggct gacctcgcgc accagcgcac
     5701 ttcggtgcgc gtgagcgggt gggacgtgtc gagcaagagc gcgctcgacg aggaggcggg
     5761 cgtctcggtc gtcaacggcg agctggacgg ccggcggagc gggagcgccg tgctcgccgc
     5821 ggcgctggcc gagcgccacg agcgcgtcgt gatgacggtg ccgctctcgc agcccgaggc
     5881 gaaggccgtg gccgaggcgc gctaccgcgc gcgcgcgcgc cgcttcgtgc gcggcagggg
     5941 ccaggccgac ggcacgcccg agctgcgcgt cggcacgtcg ctcactttgg gcggcctcgg
     6001 cgatttcttc gacggcaagt
//
LOCUS       DBNGOH010000331        10601 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4083645, whole genome shotgun sequence.
ACCESSION   DBNGOH010000331 DBNGOH010000000
VERSION     DBNGOH010000331.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 10601)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10601)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10601
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4083645"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..247
                     /locus_tag="AC159P_06420"
     CDS             <1..247
                     /locus_tag="AC159P_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015723650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HIR3695126.1"
                     /translation="DTNCGATTLPGPQTTCGTGSFFFSVNHLSIDPGQIASITGQITG
                     AQQQSAPVPEPATMLLLGTGLTGIAALARRRKLRNKA"
     gene            438..1997
                     /locus_tag="AC159P_06425"
     CDS             438..1997
                     /locus_tag="AC159P_06425"
                     /inference="COORDINATES: protein motif:HMM:NF015140.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007154 - cell communication [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Calx-beta domain-containing protein"
                     /protein_id="HIR3695127.1"
                     /translation="MIGIEFRTRRFAFARALAAAALLLLFGSAWPARVGQAQTTFTLG
                     GRIDDTGLGTGTGISGVTVSLNDINTGTLLATRQTDAAGDYSFTGLAAGGNYELVPTK
                     AGFVFNPTSRGRFNLNQNFTSEHFRGTSTTAGTVGFSAATYSVNETIGADGLGTEGSG
                     FRVVTVARTDTTGAASVDYATSDGTADRRRDYTQTLGTLRFAAGEATKTFTVLITDDR
                     YAEPNETVNLTLSNPVGMTLGAQSSAVLTIVSDDAVGGPSPVKGGSFDARFFVRQHYL
                     DFLSREPDLGGLNFWVGQTTGCGDPNPVACRINVSAAFFLSIENQETGLLAYRMFKAA
                     YGDATSPGVPGTVPIIRIHEFLADSQRIGQNVVVGTPGWPERLEANKNAYAAEFVLRQ
                     RFLTAFPGTLTAAQFVDTLNANAGGALSQSERDTLVNALAGGTMSRAQVLRAVAEDET
                     LRQAETNRAFVLLQYYGYLRRNPDDAPDTNFGGWFFWLDKLNQFNGNFVDAQMVQAFL
                     DSIEYGDRFGH"
     gene            2373..4829
                     /locus_tag="AC159P_06430"
     CDS             2373..4829
                     /locus_tag="AC159P_06430"
                     /inference="COORDINATES: protein motif:HMM:NF024816.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3695128.1"
                     /translation="MNRQECPRARSLRALLLVLVAIFAASAASCTNPETAKANHVSRG
                     EAYLKERKWQEAVLEFRNAVQIDDKLAAAHWGLAQAYEQLGRGVEALEALQKTVEFDP
                     ANAGARLKLANAYIVGYDRHKNQEFIAQAERLANEILAQDEKNVDGQILMANVLHLKG
                     DSEAALAKLKYAISLKPERIESHIGLAKFFIQTNRPADAEATFKQAVSINDRSSLAHV
                     ELARFYAQTRRVDEAETHFRRAAEVDPENRDVRWVLASFYLLTSRLDKAEEAYKAWAL
                     LDWDKPEGKARLADYYATVGRFDEAANLYQEIISAAPDYARGRYRLGEISLQRGDTKS
                     ADAQVADLLKRNERDMDALFLRARMRLNTGKVKDSITDLKTVLEQDPRSELGLYFMSE
                     ALYRDGQLEQARTRAGELERFHPNFLPAKLIQIQLNFDSGDAATAKRLADELLARLAQ
                     TAPDGKQTPQLLADVKTNALVLRGRANLRLKQAAAARADFEAARNVSPNSPTPHVNLA
                     DAAFAEGKRDEAAQHIERALALDRANFQAINGLINLSNVEKRLDAARARIEQLVSEQP
                     SNAQLHFLLGNAYRAGNTPPPDAAGQGGGAGQAPDPQRAEAAYRRAVELDPDYIAAYS
                     ALAEIYFAQQRPDDAVAQYGKIAERRPDNSAAYHNIGMIESGRNNHDAAEQFYRRALS
                     VQPDEPISANNLAMLYADHGKGNPDEAMRLAQEVVRRFPDEPGFADTLGWVYYKKGLH
                     AAAVEQLDKAVKAAAARGGDNSLYRYHLGMALAARGDKARARAELQKCLQLAADEEKR
                     PVKAPTTTPLDEVRRTLESL"
     gene            4933..6558
                     /locus_tag="AC159P_06435"
     CDS             4933..6558
                     /locus_tag="AC159P_06435"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: protein motif:HMM:NF014732.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GumC family protein"
                     /protein_id="HIR3695129.1"
                     /translation="MSVEFRQRTPGEYARMVWRRKWLIVLPTVAVGLAVAYVVWKLPN
                     TYESTTLLTVRPASISTSVVPQLSESDLTIRINNIGQEVTSRSSLEPLIERYGLYAAE
                     RRRGEPMDLLVERMRTQDVGVQINQSRNDITNGFYLSFRGSDPRTTQAVTAELASKYI
                     NAQTSAAQASSTQTVDFFEQRLKEAKEELDAIDRRRLEVMMKNRDSLPSSMDALIGQL
                     AGLREEQQGIMSALAVLRERNSMLGTQRADLEKQREQEINNVAEQVGDPKQTLAYAEL
                     VKRKAALESEQAQMAPVLKPKHPDMIAKKSEIDSVQRGMDAMLAETKEKVAEKRKKLE
                     SQIDPRLNSIKYELQSVTAQAAILEKRMAANEGQIADISRRLNGVPGTTVELEAINRD
                     FMAKKGVHDELLAQKGKADLVAGVTANAQGESIAVIDAASLPQQPVAPNRLLLAVLGL
                     AAGLACGLALAAAFEVPRLLTIETSEDAAHYTGLPVLIALPNMLTAGEQRRLRLRRAA
                     LGVAGLAVAVLSVPALIIMLRLTHIVEMLASRG"
     gene            6680..7756
                     /locus_tag="AC159P_06440"
     CDS             6680..7756
                     /locus_tag="AC159P_06440"
                     /inference="COORDINATES: protein motif:HMM:NF024793.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ExeA family protein"
                     /protein_id="HIR3695130.1"
                     /translation="MYLDFYGLKELPFALTPDPRFIYFTPSHAEAMANLHYGIESGKG
                     LIVVTGEVGTGKTTMLRWIMQRLDRTVLVAYIFNPRMTVSEFYQYVSSPKLLDVPSWE
                     NKSELLIRLGEVLESRHARGLRTVLIIDEAHGLSPAVLEEIRLLSNYESDSAKQLQII
                     LTGQPELRSVLNNPALRQLKQRIALRCDIKQLPNIEETERYIASRLLAAGAERTDIFT
                     PAAVDFVYRCSEGIPRQINNLCDNALLAGFAAGVEEIDRQIIEEVAETFDLLPGGQRG
                     MQTGEERERSSRVFGPAARAELWAVGAAAEEGRRGVAGAAQAHEERELEREFEVGGGG
                     ATGRPTPDSDELGRAFTRWDERGD"
     gene            7759..8850
                     /locus_tag="AC159P_06445"
     CDS             7759..8850
                     /locus_tag="AC159P_06445"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HIR3695131.1"
                     /translation="MGRVYEALKRAAEAKAANGANGANGKPHEEAAPSSPSPHAKNGG
                     TPAAEPKPNGHGAEKPAALTPETLFETATHFKSPETAHVSAPTGHTGPPTGSALPGGT
                     APREAGATLGAVGSARTPDFSTLEISAARVEPHLVSITQPRSAHAERYRALRTRVLHA
                     GERKKLQAFVVTSAGIMEGKTLTATNLAWLLAQTDGVRALLIDGDLRNPCAADYLGVD
                     YDLGLSEVLAGEATLEEAILRIEPAGLHLLPGGAVRDDVAEVLSGPKFSGLLAEARKM
                     FDYIIIDAPPLSLFTDATVLISRADSALIVARAGKTRYAALERVLEPVARERILGVVL
                     NGSDEELSESNYNYYRRRYQTREAAAEDV"
     gene            8900..10288
                     /locus_tag="AC159P_06450"
     CDS             8900..10288
                     /locus_tag="AC159P_06450"
                     /inference="COORDINATES: protein motif:HMM:TIGR03013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03013 family XrtA/PEP-CTERM system
                     glycosyltransferase"
                     /protein_id="HIR3695132.1"
                     /translation="MSPTRFNIRTVLLIIAESLLVFGAVVAAAYVRFGFDDAAIELNE
                     KYGFLKAALAASFCLTAFYFYDLYDFVVMHDRRELVLRLIQALGLAWVALAIVMYLVP
                     QLFIGRGVSLFALPLALTLMVAWRVSIHWLLGHPNVGERILIVGSGEEAIEIAREVLD
                     RRDAGYRVAGFVDNRPELVGQSILNPRVLGTSAEMAKIVRREGVNRIVVAMGERRGQF
                     PVRQLLDLSLSGDVAIEECASFYERLTGRVHLDMLRPSWLIFSGRGRQGRLSAAVRTV
                     VHRGVALIGAVVSLPVALLTALLIKLESPGPVLYRQERVGKHGRTFSIMKFRSMREDA
                     ESNGPVWASTQGDARTTRVGRVIRKVRVDEIPQFWNIMKGDMNFVGPRPERPHFVQQL
                     AEEIPFYEQRHLIPPGLTGWAQIKYPYGASVEDARQKLQYDLYYIKNQSLVLDAVIMF
                     ETVKTILFGRGT"
     gene            10315..>10601
                     /locus_tag="AC159P_06455"
     CDS             10315..>10601
                     /locus_tag="AC159P_06455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695133.1"
                     /translation="MKLYPKLAASIALAIIIIAPHAATPASARQTGQAEQKKEESKKT
                     GAQPPAKAGEERLEPDDARPARGGVPDETVANRREGAREDDEAEVVYYNNFL"
BASE COUNT         1887 a         3796 c         3466 g         1452 t
ORIGIN      
        1 ggacacgaac tgcggggcga cgaccctccc ggggccgcag acgacctgcg ggaccgggtc
       61 gttcttcttc tctgtcaacc acctgtcgat cgaccccgga cagatcgctt cgatcaccgg
      121 gcagatcacg ggcgcgcagc agcagagcgc ccccgtgcct gagccggcga cgatgctcct
      181 gctcgggacg gggctgacag gtatcgccgc cttggcgcgc cgccgcaagc tccgcaacaa
      241 ggcttaacac gttcgtttta cccggcacaa gctgaagaca cggacgggag gcaccggcgg
      301 ccagtaacgc gccggaacgc ctcccgtccg cgttcgtcac gcgcgagatc gcaggacgcc
      361 gcaggccgcc acggagtata ataggccagt cactcgggcc gaacgcgccc gcacttcacg
      421 tccacaaagg ggatttcatg atcggtatcg agtttcgcac acgccgcttt gcctttgccc
      481 gcgcccttgc cgcggcggcg ctcctcctcc tcttcggctc ggcgtggccg gcccgcgtcg
      541 gtcaggcgca gacgaccttc accctcggcg ggcgcatcga cgacacgggc ctcggcacgg
      601 ggacgggcat ctccggcgtc accgtgtcgc tgaacgacat caacacgggc acgctgctgg
      661 cgacccgcca gaccgacgcc gcgggcgact attctttcac gggtctggcg gcgggcggca
      721 actacgagct cgtgccgacg aaggccgggt tcgtcttcaa ccccacctcg cgcgggcgct
      781 tcaacctgaa ccagaatttc acctccgagc acttcagagg caccagcacg accgccggca
      841 cggtcggctt cagcgccgcc acctacagcg tgaacgaaac aatcggcgcg gacggcctcg
      901 ggacggaggg cagcggcttt cgcgtcgtca ccgtggcacg caccgacacc acgggggcag
      961 cctccgtgga ctacgccacc tcggacggca cggcggacag gcgcagggat tacacgcaga
     1021 cgctcggcac gctccgcttc gccgcgggcg aggcgaccaa gaccttcacc gttctcatca
     1081 ccgacgaccg ctacgcggag ccgaacgaga ccgtcaacct caccctcagc aacccggtcg
     1141 ggatgacgct cggcgcgcag tcgagtgccg tcctcaccat cgtcagcgac gacgccgtcg
     1201 gcgggccgag tccggtcaag ggcgggagct tcgacgcgcg gttcttcgtc cgccagcact
     1261 acctcgactt cctcagccgc gagcctgacc tcggcggcct caacttctgg gtcggccaga
     1321 cgaccggctg cggcgacccg aaccccgtcg cctgccgcat caacgtctcg gcggccttct
     1381 tcctctcgat cgagaatcag gagacgggcc tcctcgccta ccgcatgttc aaggcggcct
     1441 acggcgacgc cacctcgccg ggcgtgcccg gcaccgtgcc catcatccgc atccacgagt
     1501 tcctggctga ctcgcagcgc atcgggcaga acgtggtggt cgggacgccg ggctggccgg
     1561 agcggctcga agcgaacaag aacgcctacg ccgccgagtt cgtcctgcgg cagcggttcc
     1621 tcacggcttt ccccggcacg ttgacggcgg cgcagttcgt ggacacgctc aacgcgaacg
     1681 cgggcggcgc cctctcgcag tcagagcgcg acacgctcgt caacgcactg gcgggcggca
     1741 cgatgtcgcg cgcgcaagtc ctgcgcgccg tcgccgagga cgagacgctg cggcaggccg
     1801 agaccaaccg cgccttcgtg ctgctacagt actacggcta cctgcgccgc aacccggacg
     1861 acgcgcccga caccaacttc ggcggctggt ttttctggct cgacaagctc aaccagttca
     1921 acggcaactt cgtggacgcg cagatggtgc aggcgttcct cgactccatc gagtacggcg
     1981 accgtttcgg gcactagccg ccccggcccc gaggctgtcg gccctcgggc cgccccggaa
     2041 aaagatagtg cgttacggga aacaggccgc gattgagtat ttgtagacac cgtacgctcc
     2101 cgaagaagcg ggaagcgaga gagcttgaca aggtcggagc cgggacgtta accggagacg
     2161 agcgcgcgtg ccccgcgccc cgtccgggcg cgtttttgtt ggactttact aaccggcttc
     2221 gtgttaccat aagtcgcagg acgcgcgcgg cattgcccgc cgccgcagcc cgcgaccgaa
     2281 ctaatccaag agggcgactc gaacgccgaa cgaaaccggt gccccggttt cgccgccgcg
     2341 ggtcgtccga caaccaagga gtgccacgcc agatgaatcg tcaagagtgc cctcgcgcgc
     2401 ggagcctccg cgcgctgctg ctcgtcctcg tcgccatctt cgccgcctcc gcggcatcat
     2461 gcacgaaccc cgagaccgcg aaggccaacc acgtcagccg cggcgaagct tacctcaagg
     2521 agcgcaagtg gcaggaggcc gtgctcgaat tccgcaacgc cgtccagatc gacgacaagc
     2581 tggccgccgc gcactggggt ctcgcgcagg cttacgagca actgggccgc ggcgtcgagg
     2641 cgctcgaagc gttgcagaag accgtggagt tcgacccggc caacgccggc gcccgcctca
     2701 agctcgccaa cgcctacatc gtcggctacg accgccacaa gaatcaggag ttcatcgcgc
     2761 aggccgagcg gctggcgaac gagattctgg cgcaggacga gaagaacgtt gacggccaga
     2821 ttctgatggc caacgtcctg cacctgaagg gcgacagcga ggcggcgctc gccaagctga
     2881 agtacgccat cagcctcaaa cccgagcgca tcgagtcgca catcggcctc gccaagttct
     2941 tcatccagac caacaggccg gcggacgccg aggccacctt caagcaggcc gtctcgatca
     3001 acgaccgttc gtcgctcgcg cacgtcgagc tggccaggtt ctacgcgcag acgaggcggg
     3061 tggacgaggc cgagacgcac ttccgccgcg ccgccgaggt cgatcccgag aaccgcgacg
     3121 tgcgctgggt gctcgccagc ttctacctcc tcaccagccg gctcgacaaa gccgaggagg
     3181 cttacaaggc gtgggcgctg ctcgactggg acaagcccga gggcaaggcg cgcctcgccg
     3241 actactacgc gacggtcggg cgcttcgacg aggcggcgaa cctctaccag gagatcatca
     3301 gcgccgcgcc cgactacgcg cgcgggcgct accgcctcgg cgagatcagc ctccagcgcg
     3361 gcgatacgaa gagcgccgac gcacaggtcg ccgatctgct caagcgcaac gagcgcgaca
     3421 tggacgcgct cttcctgcgc gcgcggatgc ggctcaacac gggcaaggtg aaggacagca
     3481 tcaccgacct gaagacggtg ttggagcaag acccgcgctc ggagctgggc ctctacttca
     3541 tgtccgaggc gctctaccgc gacggccagc ttgagcaggc gcgcacccgc gccggcgaac
     3601 tcgaacgctt ccaccccaac ttcctcccgg ccaagctcat ccagattcag ctcaacttcg
     3661 actcgggcga cgcggcgacc gccaagcgcc tggccgacga gctgctcgcg cgcctcgcgc
     3721 agaccgcgcc cgacggcaag cagacgccgc agctcctcgc cgacgtgaag acgaacgcgc
     3781 tcgttctgcg cggcagggcc aacctccggc tgaagcaggc ggcggcggcg cgcgccgact
     3841 tcgaggccgc gcgcaacgtc tcgcccaact cgcccacccc gcacgtcaac ctcgccgacg
     3901 cggccttcgc cgagggcaag cgcgacgagg cggcgcagca catcgagcgc gcgctcgcgc
     3961 tcgaccgcgc gaacttccag gccatcaacg gactgatcaa cctctcgaac gtcgagaagc
     4021 ggctcgacgc cgcgcgcgcg cgcatcgagc agctcgtcag cgagcagccc tccaacgcgc
     4081 agctccattt cctgctcggc aacgcctacc gcgccgggaa cacaccgccg ccggacgcgg
     4141 cggggcaggg cggcggcgcc ggccaggcgc ccgacccgca acgcgccgaa gcagcgtacc
     4201 gccgcgccgt cgaactcgac cccgactaca tcgccgccta ctcggcgctc gccgaaatct
     4261 acttcgcgca gcagcgcccg gacgacgccg tcgcgcagta cggcaagatc gccgagcgcc
     4321 gcccggacaa ctccgccgcc taccacaaca tcggcatgat cgagtcgggg cgcaacaacc
     4381 acgacgcggc agagcagttc taccgccgcg ccctctccgt gcagcccgac gaacccatct
     4441 ccgccaacaa cctcgccatg ctctacgccg accacggcaa gggcaacccc gacgaggcga
     4501 tgcgtctcgc gcaggaggtc gtccgccgct tcccggacga gccgggcttc gccgacacgc
     4561 tcggctgggt ctactacaag aagggcctgc acgcggctgc cgtcgagcag ctcgacaagg
     4621 cggtcaaggc ggctgccgcg cgcggcggcg acaactcgct ctaccgctat cacctcggca
     4681 tggcgctcgc ggcccgcggc gacaaggcgc gcgcgcgcgc cgagctacag aagtgcctcc
     4741 agctcgccgc cgacgaggag aagcggcccg tcaaagcgcc gaccacgacg cccctcgacg
     4801 aagtgcgccg gacgctggaa agcctgtaag agccgtgaac cggaaaccgg aaaccgtgac
     4861 gagacaaaac ccgtctccct ctcgtcacgg ttcgcggttc acggttcacg gcactccgaa
     4921 ggagtgcaga agatgagtgt tgaattcaga cagcggacgc cgggcgagta cgcgcggatg
     4981 gtgtggcggc ggaagtggct gatcgtgctg ccgacggtgg cggtcggcct cgcggtcgcg
     5041 tacgtcgtgt ggaagttgcc gaacacctac gagtccacga cgctcctgac ggtgaggccg
     5101 gcgagcatct cgacctcggt cgtgccgcag ctctcggaga gcgacctcac gatccgcatc
     5161 aacaacatcg ggcaggaggt gacgagccgc agctcgctcg aaccgctgat cgagcgctac
     5221 gggctgtacg ccgccgagcg ccgccgcggc gagccgatgg acctgctcgt cgagcggatg
     5281 cgaacgcagg acgtgggcgt ccagatcaac cagtcgcgca acgacatcac gaacggcttc
     5341 tacctctcgt tccgcggctc cgacccgcgc acgacgcagg ccgtcacggc cgagctggcc
     5401 tcgaagtaca tcaacgcgca gacgagcgcc gcgcaggcct cctcgacaca gaccgtggac
     5461 ttcttcgagc agcggctcaa ggaggccaag gaggaactcg acgccatcga ccgccggcgg
     5521 ctcgaagtga tgatgaagaa cagggacagc ctgccgagca gtatggacgc gctgatcggc
     5581 cagctcgccg gcctgcgcga ggagcagcag ggcatcatgt ccgcgctcgc cgtcctgcgc
     5641 gagcgcaact cgatgctcgg cacgcagcgc gccgacctcg aaaagcagcg cgagcaggag
     5701 atcaacaacg tcgccgagca ggtcggcgac ccgaagcaga cgctggccta cgccgagctg
     5761 gtcaagcgca aggcggcgct ggagtccgag caggcgcaga tggcgccggt gctgaagccg
     5821 aagcacccgg acatgatcgc caagaagtcc gagatcgaca gcgtgcagcg cggcatggac
     5881 gccatgctcg ccgagaccaa ggagaaggtc gccgaaaagc ggaagaagct cgaatcgcag
     5941 atcgacccgc gcctgaactc catcaagtac gagttacagt ccgtcaccgc gcaggccgcc
     6001 atcctggaga agcggatggc cgcgaacgag ggccagatcg ccgacatcag ccgccgcctt
     6061 aacggcgtgc ccggcacgac ggtcgaactc gaagccatca accgcgactt catggcgaag
     6121 aagggcgtcc acgacgagct gctcgcgcag aaagggaagg ccgacctcgt ggcgggcgtg
     6181 acggccaacg cgcagggcga gtccatcgcg gtcatcgacg cggcgagcct gccccagcag
     6241 cccgtcgcgc cgaaccggct gctgctcgcc gtgctcggcc tcgccgcggg gctggcgtgc
     6301 ggcctcgccc tcgccgccgc cttcgaggtg ccgcgcctgc tgaccatcga gacgagcgag
     6361 gacgcggccc actacacggg cctgcccgtg ctcatcgcgc tgcccaacat gctgacggcg
     6421 ggcgagcagc gccgcctccg cttgcgccgc gccgcgctcg gcgtcgccgg cctggcggtc
     6481 gccgtcctca gcgtccccgc gctcatcatc atgctcaggc tgacgcacat cgtggaaatg
     6541 ttggcttcca gaggatgaag gaaaacgcag aatgcagaat gcagagtgca gaacgatgaa
     6601 ttaaaggcta cttgaatgac gagaggcacg gtgcttctca tcattctgca ctcagcattc
     6661 tgcacttcgg agagacccaa tgtatctcga cttctacgga ttgaaagagc tgcccttcgc
     6721 gctgacgccc gacccgcgct tcatctactt cacgccgtcg cacgccgagg cgatggccaa
     6781 cctgcactac ggtatcgaga gcgggaaggg gctgatcgtc gtgaccggcg aggtcgggac
     6841 ggggaagacg acgatgctgc gctggatcat gcagcggctc gaccgcacgg tgctcgtcgc
     6901 ctacatcttc aacccgcgga tgaccgtctc ggagttctac cagtacgtct cgtcgccgaa
     6961 gctgctcgac gtgccgagct gggagaacaa gtcggagctg ctcatccgcc tcggcgaggt
     7021 gctcgagtcg cgccacgcgc gcggcctgcg caccgtgctc atcatcgacg aggcgcacgg
     7081 cctctccccg gcggtgctcg aagagattcg cctgctctcg aactacgagt cggactcggc
     7141 caagcagttg cagatcatcc tcacggggca gcccgagctg cgctccgtgc tgaacaaccc
     7201 ggcgctccgg cagctcaagc agcgcatcgc cctgcgctgc gacatcaagc agttgccgaa
     7261 catcgaggag accgagcgct acatcgcctc gcgcctgctg gcggcgggcg ccgagcgcac
     7321 cgacatcttc acgcccgccg ccgtggactt cgtctaccgc tgctccgagg gcatcccgcg
     7381 gcagatcaac aacctctgcg acaacgcgct gctcgcgggc ttcgccgcgg gcgtcgagga
     7441 gatcgaccgc cagatcatcg aggaggtcgc cgagaccttc gaccttttgc cgggcgggca
     7501 gcgcggcatg cagaccggcg aggagcgcga acgctcctcg cgcgtcttcg gccccgcggc
     7561 gcgggccgag ttgtgggcgg tgggggccgc cgccgaagaa gggcgcaggg gcgtcgcggg
     7621 cgccgcgcag gcgcacgagg agcgagaact ggaacgcgag ttcgaagtcg gcggcggcgg
     7681 cgcgacgggt cgcccgacgc ccgactcgga cgagcttggc cgcgcgttca cacgctggga
     7741 cgagcgagga gactagcaat gggcagagtt tacgaagcac tcaagcgcgc cgcggaagcg
     7801 aaggcggcca acggcgcgaa cggcgcgaac ggcaagccgc acgaggaagc cgcaccttcc
     7861 tcgccctcac cgcacgcgaa gaacggcggc acgcccgccg cggagccgaa gccgaacggc
     7921 cacggcgcgg agaagcccgc cgccttgacg ccggagaccc tgttcgagac cgccacgcat
     7981 ttcaagtcgc cggagacggc gcacgtttca gcgcccactg ggcacaccgg gccaccaacc
     8041 gggtcggcac tccccggcgg gacggctccg cgtgaggccg gggcaacttt gggcgctgtc
     8101 ggttcggcgc gcacgccgga tttctcgacg ttagagattt ccgccgcgcg cgtcgaacca
     8161 catctcgtct cgatcacgca gccgcgctcg gcgcacgccg agcgctaccg cgccttgcgc
     8221 acccgcgtcc tgcacgcggg cgagcgcaag aagttgcagg cgttcgtcgt cacgagcgcc
     8281 ggcatcatgg agggcaagac gctgacggcc acgaacctcg cgtggctgct cgcgcagacc
     8341 gacggggtgc gcgcgctcct catcgacggc gacctccgca acccctgcgc cgcggactac
     8401 ctcggcgtcg attacgacct cggcctctcc gaagtgctcg ccggcgaggc gacgcttgag
     8461 gaggcgatcc tgcgtatcga accggcgggc ctgcacctgc tgcccggcgg cgcggtgcgc
     8521 gacgacgtgg ccgaggtgct gtcggggccg aagttcagcg gcctgctggc cgaggcgcgc
     8581 aagatgttcg actacatcat catcgacgcg ccgccgctct cgctcttcac cgacgccacg
     8641 gtgctcatca gccgcgccga cagcgcgctc atcgtcgcgc gcgcgggcaa gacgcgctac
     8701 gccgcgctcg aacgcgtcct cgaacccgtc gcgcgcgagc gcatcctcgg cgtcgtcctc
     8761 aacggctcgg acgaggagct gagcgagtcg aactacaact actaccgccg ccgctaccag
     8821 acgcgcgagg cggcggcgga agacgtgtaa ggggagtggg gcgggcgtct ctcggcgccc
     8881 gacgtgaggg ccgaatgcct tgagtccgac gcgcttcaac atccgcacag tgctgctcat
     8941 catcgccgag tcgctgctcg tcttcggcgc ggtggtggcc gccgcgtacg tgcgcttcgg
     9001 gttcgacgac gcggccatcg agctgaacga gaagtacggc ttcctcaagg cggcgctcgc
     9061 cgcctcgttc tgtctcaccg cgttctactt ctacgacctc tacgacttcg tcgtcatgca
     9121 cgaccggcgc gagctggtcc tgcgcctcat ccaggcgctg ggcctcgcgt gggtcgcgct
     9181 ggccatcgtc atgtacctcg tgccgcagct cttcatcggg cgcggcgtct cgctcttcgc
     9241 gctcccgctc gcgctcacgc tgatggtcgc gtggcgcgtc tcgatccact ggctcttggg
     9301 ccacccgaac gtgggcgaga gaatcctcat cgtcggctcg ggcgaggagg ccatcgagat
     9361 cgcacgcgag gtgttggatc gccgcgacgc cggctaccgc gtcgcgggct tcgtcgataa
     9421 ccggcccgag ctggtcggcc agagcatcct caacccgcgc gtgctcggga cgagcgccga
     9481 gatggcgaag atcgtccgcc gcgagggcgt caaccgcatc gtcgtggcga tgggcgagcg
     9541 gcgcgggcag ttccccgtcc gccagctcct cgacctgagt ctgtcgggcg acgtggcgat
     9601 cgaagagtgc gcttcgttct acgagcggct gacggggcgc gtgcacctcg acatgctgcg
     9661 cccgtcgtgg ctcatcttct cggggcgcgg caggcagggg cgcctgagcg ccgccgtcag
     9721 gacggtcgtc caccgcggcg tggcgctcat cggcgcggtc gtgtcgctgc ccgtggcgct
     9781 cctgacggcg ctgctcatca agctcgaatc gcccggcccc gtcctctacc ggcaggagcg
     9841 cgtcggcaag cacgggcgca cgttctcgat catgaagttc cgctcgatgc gcgaggacgc
     9901 cgaaagcaac ggccccgtct gggcgagcac gcagggcgac gcgcgcacga cccgcgtcgg
     9961 gcgcgtcatc cgcaaagtcc gcgtggacga aatcccgcag ttctggaaca tcatgaaggg
    10021 cgacatgaac ttcgtcggcc cccgccccga gcgcccgcac ttcgtccagc agctcgccga
    10081 ggagattccg ttctacgagc agcggcacct gatcccgccc ggcctcacgg gctgggcgca
    10141 gatcaagtac ccctacggcg cctcggtcga ggacgcgcgg cagaagctcc agtacgacct
    10201 ctactacatc aagaaccaga gcctcgtgct cgacgccgtc atcatgttcg agacggtgaa
    10261 gacgatcctc ttcgggcgcg gcacgtagcc gagaaccgat tggagattca agcaatgaag
    10321 ttatatccca aactcgcggc cagcatcgcg ctcgccatca tcatcatcgc gccgcacgcg
    10381 gcgacgccgg cctcggcgcg gcagacgggg caggccgagc agaagaaaga ggagtcgaag
    10441 aagaccggcg cgcagccgcc cgcgaaggcc ggcgaggagc gccttgagcc ggacgacgcg
    10501 cggcccgcgc gcggcggcgt gcccgacgag acggtcgcca accgccgcga gggcgcgcgc
    10561 gaggacgacg aggccgaggt cgtctactac aacaacttcc t
//
LOCUS       DBNGOH010000332         5084 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3350405, whole genome shotgun sequence.
ACCESSION   DBNGOH010000332 DBNGOH010000000
VERSION     DBNGOH010000332.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5084)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5084)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5084
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3350405"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..720
                     /locus_tag="AC159P_06460"
     CDS             <1..720
                     /locus_tag="AC159P_06460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695134.1"
                     /translation="REVRAADCPLCGELPDELIVRTGRDQRFPAAFNKLVPVGREYTG
                     RATSSQDYRCPDCGADFVCEDYTQQTGSGINDEVWIIRAPAEAPVSHRAHGPSYDYDA
                     AAAKLAAEAEAAYARQAFGIMEKHFAGAGVVMKHPDPPKDWSQFCSRFYLNTVEFAER
                     GSHTRLMAGMLAELVDLMYASPGRKFINDLYRLLVDFRHYSWLEPLLPDAARSRIDDM
                     VEMRSDAEDEDERFRLGQMSY"
     gene            929..1528
                     /locus_tag="AC159P_06465"
     CDS             929..1528
                     /locus_tag="AC159P_06465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695135.1"
                     /translation="MAEQYFNQAAAEWARRNLAREIVIELRGNSLSARIVVALALNCL
                     GVIFFGIPAILLLNAAAKFVAHGWGAEVSKPLRLGIIFLAGFGGLAGFLFSYVWTLRR
                     KLVRFLSAGGVTTRGGKEYRWESLQHLNHVRFYRQMSRNVVLRAVSNFIFAGVENLRI
                     EMVFTDGTATLAPLVENRREVLDLIDTIPARRKSEVRTH"
     gene            complement(1646..1777)
                     /locus_tag="AC159P_06470"
     CDS             complement(1646..1777)
                     /locus_tag="AC159P_06470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695136.1"
                     /translation="MRLSIMELRYASVMRQSITLALGACWEKRLTAAQKRFNRACEL"
     gene            complement(2015..2356)
                     /locus_tag="AC159P_06475"
     CDS             complement(2015..2356)
                     /locus_tag="AC159P_06475"
                     /inference="COORDINATES: protein motif:HMM:NF042668.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6616 family protein"
                     /protein_id="HIR3695137.1"
                     /translation="MYLYVELWKTRPAWLALSREQRAAWMDKLLAGLQEHLQSGVEVL
                     GFARGDADTPLPAGYDFLAAWRMPGREAAERFERFVEGAGWHEYFEQVNARGRVMETA
                     EFVSSHLDAGG"
     gene            complement(2382..4427)
                     /locus_tag="AC159P_06480"
     CDS             complement(2382..4427)
                     /locus_tag="AC159P_06480"
                     /inference="COORDINATES: protein motif:HMM:NF047446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF7619 domain-containing protein"
                     /protein_id="HIR3695138.1"
                     /translation="MSNAQNGLGAAQACQLGPDGFARNETRFVFSCDPNDKVGSSGAG
                     AAQFIAGDVPIPYAVFFENKPEASVPAQDVVITDQLDTSKFDLSTFQLGAISFGDKTV
                     VPPPGRSEFNTEVDLRPDKELIVRVNAKIDKTTGLLTWRFASLDPATLQPTLDPLAGF
                     LPPNRVPAEGEGSVLFTVSPKTDLASGTEIRNGARIVFDTNAPIDTPVWLNTIDKTKP
                     GSGVQPLGAAQSSESFTVQWSGADAHSGVANFSVFVSEDGGPFQPWLRNTTETSAVFA
                     GRQGKTYGFYSLARDNTGNVEGAKTAAEATTTVNTDGGSPVTTATLSHPANAAGWHNS
                     NVTVSLSASDAGSGVEEIIYTSTGAQAIVGETVAGASASIPVTAEGVTEITFFAKDNV
                     GNTESAQTIVVRLDKTAPAVTCAPPDGLWHAADVSINCTADGGVSGLADPADASFSLS
                     TSVAPGAETAQAATGSRAVTDLAGNAAVAGPVGGNKVDKKAPSIIITSPAAGVSYLLN
                     QAVAASYACADGGSGVGGCAGTVPNGSPLDTAVAGAKVYAVNASDAAGNATALSVTYA
                     VTYGVLALHDETRAHRGGSVIPIKLRLVDANGVNVSSQNIALTALGTSRLSDEAPGEV
                     ADAGAANPDYNFRFTTFDAAGGYIYNLNTSGLATGTYVLTFRVAGDTMTYATRFSIR"
     gene            4675..5022
                     /locus_tag="AC159P_06485"
     CDS             4675..5022
                     /locus_tag="AC159P_06485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695139.1"
                     /translation="MKSVSQPGRGGGGGGGGGGGGGGLKGVGEGVGVSVGVGVALGMG
                     VGEGVGVAVGVGVGVDVGEGVGVGVGASLRTETFRLKKCLVGLVASASPPPPPPPPPP
                     SPVSRQAGLSGGT"
BASE COUNT          834 a         1587 c         1704 g          959 t
ORIGIN      
        1 cgcgaagtcc gggcggcgga ttgccctctc tgcggggagc tccccgatga attgatcgtc
       61 agaacgggtc gggatcagcg ctttcccgcc gcctttaaca aacttgtccc ggtgggccgt
      121 gagtacaccg gtcgggcgac gtcttcacag gactaccgct gtcctgactg cggcgctgat
      181 tttgtctgcg aagattatac gcaacagacc gggagcggca ttaacgacga ggtatggata
      241 attcgcgctc cggcggaggc tccggtaagc catcgggcac acggcccgtc gtacgactac
      301 gacgccgccg ccgcgaagct ggcggcggag gccgaggcgg cctacgcgcg gcaggccttc
      361 ggcatcatgg agaagcactt cgcgggcgcc ggcgtcgtca tgaagcatcc ggacccgccg
      421 aaggactggt cccagttctg ctcgcgcttc tacctcaaca ccgtcgagtt cgcggagagg
      481 ggatcgcata cgaggttgat ggcagggatg ttagccgagc tggtcgatct catgtatgcg
      541 tcccccggtc gtaaattcat taacgacctg tataggttgc tggtagattt ccgtcactat
      601 tcctggctgg agccgctcct gcccgatgcg gcgcggagta gaatagatga catggtagag
      661 atgcgttcgg acgctgaaga cgaggacgag cggttccggc tcggacagat gagctactga
      721 ttttttaccc ggcacgcaga aacggacata aggaccatca tcggcctccg cgccgcgggg
      781 acgatgatgg gtcggcgcgc cgaggcactg cacgtaagct ttaaatgatt ccagacgcga
      841 aagggcgcac ctcatcagag cgcctttcgt ttctttcgtt ttatggcaga gcccggcttt
      901 aggtgttccg tatttacccc gcttaacaat ggccgaacaa tacttcaacc aagccgccgc
      961 cgaatgggcg aggcgcaatc tggcgcggga gatcgtaatc gagctgcgtg ggaattcgtt
     1021 gtcggcccgg atcgtcgtcg cgctggcgct gaactgcctc ggcgtgatct ttttcgggat
     1081 cccggcgatt cttctcttaa acgccgccgc taaattcgtc gcccacggct ggggcgcaga
     1141 ggtctccaaa cccctgcgac tgggcatcat ttttctggca ggtttcggcg gcttagccgg
     1201 ctttctgttc tcctatgtgt ggactttgag aagaaaactc gtcagatttt tgagcgccgg
     1261 cggcgtgacg acgcggggcg ggaaagaata tcgctgggag agtttacagc acctcaatca
     1321 tgtcaggttt tatcggcaga tgagccgcaa tgttgtgttg cgggcggtga gcaacttcat
     1381 ctttgccggc gttgaaaacc tgagaatcga gatggttttt accgacggca cggcgacgct
     1441 cgcgccgcta gtcgaaaatc ggcgggaggt gctcgattta atcgacacga ttcctgcccg
     1501 gcgtaaatct gaagtccgga cacattaata aaccggcccc cccgcattct cctcgatctc
     1561 aagctacgtt gacctgctgc ccgccttcgg tcgccacgtt gatctgcacc ttcgggactc
     1621 gggccgacat tttccgcacc cgctctcaca actcgcacgc acggttaaac ctcttctgcg
     1681 ccgccgtcag ccgcttctcc cagcacgcgc ctagcgcgag cgtgatcgac tgcctcatca
     1741 cgctcgcgta gcgcagctcc atgatagaga ggcgcatcca gcacggggcg gcgtgccggg
     1801 tcaaagccct ctcgaggggc ggcgcggtcc ggaacccgag gtcgtctttt aactccccgg
     1861 aagtggaagt actctcctgg aggaaatttc ttaagaagcg gtaggctcga aactatcggc
     1921 ggaactctcc gtgttaatta agcaaggggg gcggccgccc gagtccccag actcccatgt
     1981 ttatttcttg gggattcctt ccgggtcgtg cgccttagcc ccccgcgtcg aggtgcgagg
     2041 agacgaactc tgcggtctcc atcacccgcc ccctggcgtt gacctgctcg aagtactcgt
     2101 gccaacccgc cccctcgacg aaccgctcga acctctcggc cgcctccctg cccggcatcc
     2161 gccaggcggc caggaagtcg tagcccgccg gcaggggcgt gtccgcgtcg ccccgcgcga
     2221 agcctaagac ctcgacgcct gactgaagat gctcttgcag cccggccaaa agcttgtcca
     2281 tccacgccgc ccgctgctcg cgcgagagtg cgagccaggc ggggcgggtc ttccacaact
     2341 cgacgtacag atacataccc cccccttccc gcaagaccta ctcacctgat actgaaccgc
     2401 gtggcatacg tcatggtgtc gccggcgacg cggaaagtca ggacgtaggt ccccgttgcc
     2461 agcccggacg tattcaggtt gtagatgtac cctcccgccg cgtcgaaagt ggtgaagcgg
     2521 aagttgtagt ccgggttggc ggcgcccgcg tccgcgacct cgccgggcgc ttcgtcggag
     2581 aggcgcgacg tcccgagcgc cgtcagggcg atgttctgcg acgacacgtt gaccccgttg
     2641 gcgtcgacga gtcggagctt gatggggatg acgctgccgc cccggtgcgc cctcgtctcg
     2701 tcgtggagcg cgaggacgcc gtaggtgacc gcgtaggtga cgctcagggc ggtggcgttg
     2761 cccgccgcgt cagacgcgtt cacggcgtag accttcgcgc ccgcaacggc ggtgtcaagc
     2821 gggctcccgt tcgggacggt gccggcgcac cccccgacgc ccgagccgcc gtccgcacac
     2881 gcgtagctcg cggccacggc ctggttgagg aggtagctga cgccagcggc cggcgaggtg
     2941 atgatgattg agggagcttt cttgtccacc ttgttgccgc cgacggggcc cgcgaccgcc
     3001 gcgttgccgg cgaggtccgt gaccgcgcgg ctccccgtcg cggcctgggc agtttcggcg
     3061 ccgggcgcga cgctcgtcga gagcgagaaa ctcgcgtcgg ccgggtcggc caggccggag
     3121 acgccgccgt ccgccgtaca gttgattgag acgtctgccg cgtgccagag gccgtccggc
     3181 ggggcgcacg tgaccgcggg cgcggtcttg tcgagtcgga cgacgatagt ctgagccgac
     3241 tccgtgttgc cgacgttgtc tttggcgaag aaggtgattt cggtgacgcc ctccgccgtg
     3301 accgggatgg aggccgaagc tccggcgacg gtctcgccga cgattgcttg ggcacccgtt
     3361 gacgtgtaaa tgatctcttc gacacccgag ccggcgtcgg aggcgctaag cgagacggtc
     3421 acgttcgagt tgtgccagcc ggcggcgttc gccgggtgcg agagcgtggc cgtggtgacg
     3481 gggctcccgc cgtcggtgtt gaccgtggtc gtggcctccg ccgccgtctt cgcgccctcc
     3541 acgttgccgg tgttgtcgcg ggcgaggcta tagaagccgt atgtcttgcc ctgccgtccc
     3601 gcgaagacgg cggaagtctc cgtcgtgtta cgaagccagg gctggaaggg gccgccgtct
     3661 tcggagacga agacggagaa gttcgcgacg ccggagtgcg cgtccgcacc cgaccattgc
     3721 accgtgaagc tctcggaaga ctgggcggct ccgagcggct gcacgccgct gccgggcttc
     3781 gtcttgtcga tcgtgttgag ccagacgggc gtgtcaatcg gggcgttggt gtcgaagacg
     3841 atccgcgcgc cgttgcgaat ctcggtgccg gaggcgaggt cggtcttcgg gctgacggtg
     3901 aacagaacgc tcccttcccc ctcggcgggg acgcggttgg gcgggaggaa gccggcgagc
     3961 gggtcgagcg tgggctgcaa cgtcgccggg tcgagcgacg cgaatcgcca cgtgagaagc
     4021 cctgtcgtct tatcgatctt ggcgttgacc cgcacgatca attccttgtc cggcctcagg
     4081 tcaacctccg tgttgaattc gctccttccg ggcggcggga cgacggtctt gtcgccgaag
     4141 ctgatcgcgc cgagctggaa ggtcgagagg tcgaacttcg aggtgtcgag ttggtcggtg
     4201 atgaccacgt cctgcgccgg gaccgaggcc tcgggcttgt tctcgaagaa gacggcgtaa
     4261 gggatgggca cgtcgccggc gatgaactgg gccgcacccg cgcccgaaga gccgaccttg
     4321 tcgttgggat cacacgagaa gacgaacctc gtctcgtttc tggcgaaccc gtcgggcccg
     4381 agttggcacg cctgcgccgc gcctaagccg ttttgcgcgt tggacaaggc gtcggcggcg
     4441 tctaatgctg ctttagtgcc ttcactgatc gctttcccac cgggaataaa cgaccccgcg
     4501 cattgcatgc tgctcatgac agcggtggag accgctactc cgccccgctt ggctgcaagc
     4561 gcttcgaggc agtcgttccc aggcacgaac cctctgatga agtcgataac aggcggcaga
     4621 cagtcggcaa tacgcgacac gtccacctga gtcgcccgca cgtcattctc cgagatgaag
     4681 tccgtgtcac agcccggccg cggcggggga ggaggaggcg gcggcggcgg cggcggcggc
     4741 gggttgaaag gcgtcggaga aggggtcggg gttagtgtcg gcgtcggagt tgcgctcggc
     4801 atgggagtcg gcgaaggcgt cggcgtcgcc gtcggggtgg gcgtcggcgt cgacgtcggc
     4861 gaaggcgtgg gtgtcggcgt cggcgcatct ctgcgcaccg aaaccttcag gttgaagaag
     4921 tgtctggttg ggctagtagc gtccgcctcc ccgccgcctc ctccgccgcc gcctcctccg
     4981 tcgcctgttt cacgacaggc ggggttgtcg ggcgggacgt aagccagcac ggggacggag
     5041 cgctcgcccg tccccgggtt ggtcagcacg gtgggcagag actc
//
LOCUS       DBNGOH010000333         3044 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2800420, whole genome shotgun sequence.
ACCESSION   DBNGOH010000333 DBNGOH010000000
VERSION     DBNGOH010000333.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3044)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3044)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3044
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2800420"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..270)
                     /locus_tag="AC159P_06490"
     CDS             complement(<1..270)
                     /locus_tag="AC159P_06490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695140.1"
                     /translation="MTSIARTPFAAVFRAELLYTSKRAVPYALLALFCANAVLWWGVG
                     AATHFGWATNSDFYIARNFGGFSFLTLPLFNALLMGDPVVRDYRHG"
     gene            complement(439..1323)
                     /locus_tag="AC159P_06495"
     CDS             complement(439..1323)
                     /locus_tag="AC159P_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011644561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3695141.1"
                     /translation="MLEVRDLEKTYASGVRALKSVSLGAPAGVFGLLGPNGSGKTTLM
                     KILATLLAPDSGQASVGGVDLVNDRHAARTALGYLPQEFGLYPTLTAAETLDYFARLK
                     GVAVASERRALVAALLERVNLAAEGGQQVGGFSGGMRQRLGIAQALVGRPRLIIVDEP
                     TAGLDPEERVRFHNLLSEIADDERVVLLSTHIVSDVSSLCGRMAVIRRGEIVAACTPR
                     EAVTRIDGAVWEATVARERVAPLKARFRVISTQAFEGRVRVRVLARGARPAEEFAPAV
                     PTLEDYYFSLVVQADRSN"
     gene            1884..>3044
                     /locus_tag="AC159P_06500"
     CDS             1884..>3044
                     /locus_tag="AC159P_06500"
                     /inference="COORDINATES: protein motif:HMM:NF024753.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HIR3695142.1"
                     /translation="MRSLRVASVLGAGLILLCSFVGAAAQTGGEWPQWRGASRDGISK
                     ETGLLKSWPEGGPPLLWKATGAGRGYSSVSISKGRIYTLGLRGDKEYVIAFDAATGKE
                     VWATAHGAAFRNDRGDGPRGTPTVDGDRVYSLGGGGDLSAVDAKTGKLIWQMNVLQKF
                     GGTNITWGISESPLVLGDKVLVNAGGRDASIVALNAKDGAVVWKSQGISDRSGYSSGM
                     PVQVGSTTQVIFFTHNRVVALDAKDGKLLWDYPKAANEVANAATPVIRGNRIWVSSDY
                     GNGGGLVEIKADGKAQEVYFTREMRNHHSSSVLIGDHLYGFSGGILTAMKFDTGEVAW
                     KDRSVGKGSLVFADGMLYLLSENSVVGLAEATPTGYVERGRFRLPQDSLPTWA"
BASE COUNT          508 a          988 c         1068 g          480 t
ORIGIN      
        1 gccgtgccgg tagtcgcgca cgacggggtc gcccatcagc agcgcgttga agagcggcag
       61 cgtgaggaag gagaagccgc cgaagttgcg cgcgatgtag aaatcgctgt tggtcgccca
      121 gccgaagtgc gtggcggcgc cgacgcccca ccagaggacg gcgttggcgc agaacagcgc
      181 gagcagcgca taagggacgg cgcgcttcga ggtgtagagc agctcggcgc ggaagacggc
      241 ggcgaacggc gtgcgcgcga tgctggtcat tttagtccgt ctccaaactc gacgtgcgtc
      301 atgattgtag gggcagggca agtccgcggc ccgccgcgtc cgcgtgcttc gacgttgaat
      361 gaatacgtaa gcgcgggccg cggacttgcc ctgcccctac aactatcacg tacgtctcac
      421 actcattcaa accccgttct agttcgacct gtccgcctgc acgacgaggc tgaagtagta
      481 gtcttcgagc gtcggcacgg cgggcgcgaa ctcttccgcg ggccgcgcgc cgcgcgcgag
      541 gacgcggacg cggacgcgcc cctcgaacgc ctgcgtcgag atgacgcgga agcgcgcctt
      601 gagcggcgcg acccgttcgc gcgcgacggt cgcttcccag acggcgccgt cgattcgtgt
      661 gacggcttcg cgcggcgtgc aggcggcgac gatctcgccg cggcggatga cggccatccg
      721 cccgcagagg ctcgacacat cggagacgat gtgcgtcgag agcaggacga cccgttcgtc
      781 gtcggcgatt tcggagagca ggttatggaa gcggacgcgc tcttcggggt cgagtccggc
      841 ggtcggctcg tccacgatga tgagccgcgg gcgaccgacg agcgcctgcg cgatgccgag
      901 ccgctgccgc atcccgcccg agaagccgcc gacctgctgg ccgccctcgg ccgcgaggtt
      961 gacccgttcg agcagcgccg cgacgagcgc gcggcgctcg cttgcgacgg cgacgccctt
     1021 gaggcgcgcg aagtagtcga gcgtctcggc ggcggtcagc gtcgggtaga ggccgaactc
     1081 ctgcggcagg tagccgagcg ccgtgcgcgc cgcgtgccgg tcgttgacga gatcgacgcc
     1141 gccgaccgaa gcctgacccg agtccggcgc gagcagcgtc gccagaatct tcatcagcgt
     1201 cgtcttgccc gagccgttcg gcccgagcag cccgaagacg cccgcgggcg ccccgagcga
     1261 gacgctcttg agcgcgcgca cgccgctcgc gtaggtcttc tccaggtctc ttacctcaag
     1321 catgactttg cgaaaaggcg aaacgtgtgc gagcttccgc cgcgtagcgg cgttcgaatt
     1381 tagccgtgcc ctgaagggca cggtcacgga tgcgtgagtt tggttccgtc gcgtcagcga
     1441 cgactgagac aaggttcaag cgtcgctgac gcgacgccgt gtcggggcgc tgcgactgcc
     1501 ccggccttga aaggccgggc taaattcaaa cgccgctacg cggcaacaga attctctcgc
     1561 cgtgtggtga cgacgcgtgg cggtgtgcgc gtgtgcttcc gatctttagc gcatattcac
     1621 ggattcatac tttatgcatt cccgcggacg ggacgtttcg agcgaccccc gttagacacc
     1681 ggcggcggcg cggggttccg tccaagaggg ggcggggatg ggcctcactc tttacaagat
     1741 tttacatcca tccggcaaga gcgccgttat aatctcaggc cgtctccaca gacgttttat
     1801 ccgcgcggac ggcgccgccc gtccgcgctt cacgatatca aaaccaaccc ggtcgcttca
     1861 gccgaagaag gagattgcta gccatgagaa gcctacgtgt cgcgtcggtg ctcggcgcag
     1921 gtctgatact cttatgttcg ttcgtcggcg cagcggcgca gacgggcggc gagtggccgc
     1981 agtggcgcgg cgcgagccgc gacggcatct cgaaggagac ggggctgctg aagtcgtggc
     2041 ccgagggcgg gccgccgctc ctgtggaagg cgacgggcgc ggggcgcggc tactcgtcgg
     2101 tctcgatctc gaaggggcgc atctacacgc tgggcctgcg cggcgacaag gagtacgtca
     2161 tcgctttcga cgcggcgacg ggcaaagagg tgtgggccac ggcgcacggc gcggccttcc
     2221 gcaacgaccg cggcgacggc ccgcgcggca cgccgaccgt tgacggcgac cgcgtctatt
     2281 cgctgggcgg cggcggcgac ctctcggcgg tggacgccaa gacgggcaag ctcatctggc
     2341 agatgaacgt gctgcaaaag ttcggcggca cgaacatcac ttggggcatc agcgagtcgc
     2401 cgctcgtcct cggcgacaag gtgctggtca acgcgggcgg gcgcgacgcc tccatcgtcg
     2461 cgctcaatgc gaaggacggc gcggtcgtgt ggaagagtca gggcatcagc gaccgctccg
     2521 gctactcttc ggggatgccc gtgcaggtcg gctcgacgac gcaggtcatc ttcttcacgc
     2581 acaaccgcgt cgtcgcgctc gacgcgaaag acggcaagct gctgtgggac tacccgaagg
     2641 ccgcgaacga ggtggcgaac gcggcgacgc cggtcattcg cggcaaccgc atctgggtct
     2701 cttcggacta cggcaacggc ggcggcctcg tcgagatcaa ggccgacggc aaggcgcagg
     2761 aggtctactt cacgcgcgag atgcgcaacc accacagcag ctcggtgctc atcggcgacc
     2821 acctctacgg tttttcgggc ggcatcctga cggcgatgaa gttcgacacg ggcgaggtcg
     2881 cgtggaagga tcgcagcgtc ggcaaaggct cgctcgtctt cgccgacggg atgctctacc
     2941 tcttgagcga gaacagcgtc gtcggactcg ccgaggccac gcccaccggc tacgtcgagc
     3001 gcgggcgctt ccgcctcccg caggactctc tgccgacctg ggcg
//
LOCUS       DBNGOH010000334         6306 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_968160, whole genome shotgun sequence.
ACCESSION   DBNGOH010000334 DBNGOH010000000
VERSION     DBNGOH010000334.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6306)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6306)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6306
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_968160"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3871)
                     /locus_tag="AC159P_06505"
     CDS             complement(<1..3871)
                     /locus_tag="AC159P_06505"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR3695143.1"
                     /translation="MGHDEQEIRFCTSADGARIAYAVVGSGPPLVKAANWLSHLEFDW
                     RSPVWRHWLRELSRGRTLIRYDERGCGLSDRDAEEFTVDAWVRDLEAVVEAAGLERFP
                     MLGISQGASIAIAYAVRHPEKVSHLILYGGYARGRLRRNPSQRQIEEAEILLQLVKVG
                     WGQENPAFRQVFTTLFIPEGTPEQLRWFNELQRVSSSPEGAARILNGFHVIDVCDLAI
                     QVNVPTLVLHGRGDLCVPFDEGRLLASLVPGARFVPLESNNHVLLESEPAWRHFLTEV
                     RDFIGAGAGRSQWTTHGMSGARETRDAEPSAAMSGTRWRRVGELFGHAVELPAGARGA
                     FLEEACGGDAELRREVESLLASDAGPGLTTRFEGALRASALSLSEESGAPPRQFVSHY
                     EVSEKLGEGGMGVVYRARDLKLERPVALKFLPDYLGADAELKRRFLQEARAAASLDHP
                     NICTVYEVGETPDGQLFIAMPCYDGETLKEKVARGALPVGEAIAYAEQAADGLAHAHE
                     AGIVHRDIKPANLFVTTQGRVKILDFGVAKVADVNTTRAGLVLGTVAYMSPEQASGET
                     VDRRTDVWSLGVVLYEMVAGVAPFRGERASAVFAQILNREPEPLAACAPGVPEELWRV
                     VSAALRKKRDERYGTAREMALDLLSLQHRLEFESRVGRGRAQEVTTEKRLAGRAVDPR
                     AASSGETAAAPSEGASSVSSVSLGPRAGGPDNLPAEPSPLIGREDELAGVLALLRRGE
                     VRLVTLTGAGGTGKTRLALAVARALAEDFGDGVFFVDLSELSGPELVASAIAQALGVK
                     EAGGTPLAEALRETLRGRAALLVLDNFEHVGGAAPLVSELLAAAPRAKALATSRSPLR
                     LRLEHEFTVPPLALPSSLSLPPTDSLVRYAAVELFVARARAVKPGFALTDGNAAVVAE
                     ICQRLDGLPLAIELAAARVRVLSPPAILARMENRLRLLTGGARDLPARQQTMRGAVAW
                     SYDLLSEDERKMLDRLSVFAGGFTMEAAEAVCEVVDEVCAEVLDCVTTLLGESLLVQR
                     ERPDGESRFRAPGVVREYGMERLRARGEAEAVERRHALYYLALSEQAEPALQGAAQAE
                     WLARLEDEHDNMRAALRWSLAREPETALRLAGSLRTFWDKRGHLSEGRGWLEAALEKA
                     PRAATTARAKALNSAGSLVWQLGDVEAAHRLYAECREVSTETGDLPLLARANNGLGIV
                     AMIRGEREAARALYEECLVVSRRLGDDQLTGVALQNLGSVVFEQGDVARARALQEESL
                     DLLRRTGNKFGVITNLINLGEVTYLQGD"
     gene            4145..4795
                     /gene="aat"
                     /locus_tag="AC159P_06510"
     CDS             4145..4795
                     /gene="aat"
                     /locus_tag="AC159P_06510"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011985154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HIR3695144.1"
                     /translation="MALVRFPDPRDSTPEGIVAVGGEPRPDFLREAYRRGIFPWPVEG
                     YPLLWFSPPERAVLDFAELHVARSLARERRRTRLRFTIDRAFERVIRACSSVERARER
                     GTWITPEVVAGSCDFHRRGDAHSVEAWEGEELVGGVYGVDAGGAFAGESMFYLRPNAS
                     KLALLFLIDHLQSRGLDWLDIQVMTPHMEALGARLISRDEFLDRLARELRRGRRLF"
     gene            complement(4851..5714)
                     /locus_tag="AC159P_06515"
     CDS             complement(4851..5714)
                     /locus_tag="AC159P_06515"
                     /inference="COORDINATES: protein motif:HMM:NF014651.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TorD/DmsD family molecular chaperone"
                     /protein_id="HIR3695145.1"
                     /translation="MELFRALAALAEPPDRPGVVRLAAALGLGDLPAASEHTEVFVFQ
                     LAPYASVYLGAEGMLGGEARDRVAGFWRALGQAPPAEADHLTLMLALYARLSEFEAGE
                     ADAARRAGWRRARGAFLWEHLLSWLPVYLDKLSEIAPPFYSRWGELLACALAEEAGKL
                     GAQDELPLHLREAPALAVDPRAYAGEDFLRTLLAPARSGMILTRADLGRGARTLGLSL
                     RAGERKFVLKALLNQDVRGVFGWLAEEAASWSRCHHTRRAEHGAVAAWWEQRAATTAT
                     LLRELEAEVDG"
     gene            complement(5818..>6306)
                     /locus_tag="AC159P_06520"
     CDS             complement(5818..>6306)
                     /locus_tag="AC159P_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012615816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase"
                     /protein_id="HIR3695146.1"
                     /translation="TEGIKPGIVACSHHMGRWKLKDEGERQMMATVSLDQRGVEWGLR
                     RERGVGPYKSSDPDTLNIWWTDVGVHQNLTFPVHPDPISGQHCWHQAVRVRKAEASDS
                     YGDIHVDTAKAREVYRHWLSQTRPADTHSPDGTRRPYWLLRPLKPSKEVYRLPEEAGS
                     RQ"
BASE COUNT          890 a         2350 c         2128 g          938 t
ORIGIN      
        1 agtcgccttg caggtaagtc acctcgccga ggttgatcag gttggtaatc acgccgaact
       61 tgttgcccgt gcgtcgcagc agatcgagcg actcctcctg aagcgcgcgc gcccgcgcca
      121 cgtcgccctg ctcgaacacc accgacccca gattctgtag ggcgacgccg gtcagttggt
      181 cgtctcccag cctgcggctc acgaccagac actcttcgta gagcgcgcgc gccgcttcgc
      241 gctcgccccg tatcatcgcc acgatcccca gcccgttgtt ggcgcgcgcg aggagcggga
      301 ggtcgcccgt ctcggtgctc acctcgcggc actccgcgta gaggcggtgc gccgcctcca
      361 cgtcgccgag ctgccacacg aggctgccgg cgctgttcag cgccttggcc cgcgcggtgg
      421 tggcggcgcg cggcgctttc tccagcgccg cctccagcca cccgcgcccc tccgacaggt
      481 gcccgcgctt gtcccagaaa gtccgcagcg acccggccag ccgcagcgcc gtctccggct
      541 cgcgcgcgag cgaccagcgg agcgccgcgc gcatgttgtc gtgctcgtct tcgagacggg
      601 cgagccactc ggcctgcgcc gcgccttgca gcgcaggctc ggcctgttcg gagagggcga
      661 ggtagtagag cgcgtgccgc cgctcgaccg cctcggcctc gccgcgcgcg cgcagacgct
      721 ccatcccgta ctccctgacc acgcccggcg cgcgaaaacg cgactcgccg tccgggcgct
      781 cgcgctgcac gagcaggctc tcgccgagca gcgtggtcac gcagtccagc acctccgcgc
      841 acacttcgtc cacgacctcg cagacagcct ccgcggcctc catcgtgaac ccacccgcga
      901 agaccgagag gcggtcgagc atcttccgct cgtcctcgct taacaggtcg tagctccacg
      961 cgacggcgcc gcgcatcgtc tgctggcgcg cgggcaagtc gcgcgcgccc ccggtcagga
     1021 gtctgaggcg gttctccatc cgggcgagga tggccggcgg cgagagcacg cggacgcgcg
     1081 cggcggccag ctctatcgcc agcggcagcc cgtcgaggcg ctggcagatt tccgcgacca
     1141 cggcggcgtt accgtccgtc agcgcgaagc cgggcttgac ggcgcgggcg cgcgcgacga
     1201 acaactcgac cgccgcgtag cgcacgagcg agtcggtcgg cggcaggctc agcgaggacg
     1261 gcagcgccag cggcggcacg gtgaactcgt gttcgagccg gaggcggagc ggcgaacggc
     1321 tcgtcgccag cgccttcgcg cgcggcgcgg cggcgagcag ctccgagacg agcggcgccg
     1381 ctccgcccac gtgctcgaag ttgtcgagga cgagcagcgc ggcccgaccg cgtaaggtct
     1441 cgcgcagcgc ctcggcgagc ggcgtcccgc cggcctcctt cacgccgagc gcttgggcga
     1501 tggccgaggc caccagctcg gggccgctca gctcggacag atcgacgaag aaaacgccgt
     1561 cgccaaagtc ctccgccagc gcgcgcgcga ccgcgagcgc cagccgcgtc ttgcccgtcc
     1621 cgcccgcgcc cgtcagcgtc accagccgca cctccccgcg ccgcagcagc gcgaggacgc
     1681 ccgccaactc gtcctcgcgc ccgatgagcg gcgaaggctc cgcgggcaga ttgtccgggc
     1741 cgcccgcccg cggcccgagc gacacggacg acacggacga cgcgccctca ctaggcgccg
     1801 cggcggtctc gccgctcgac gccgcacggg gatcgaccgc gcggcccgcg agcctcttct
     1861 cggtcgtcac ctcctgcgcg cggccacgcc ccactctgct ctcgaattcg agccggtgtt
     1921 gcaggctcag gaggtcgagc gccatctctc tggccgtccc gtagcgctcg tccctcttct
     1981 tgcggagtgc ggcggagacg acccgccaca gctcttcggg cacgccgggc gcgcacgccg
     2041 cgagcggttc aggctcgcgg ttcagaatct gcgcgaacac ggcggacgcc cgctcgcccc
     2101 ggaacggggc caccccggcg accatctcgt agagcaccac gccgagcgac cacacgtccg
     2161 tccgccggtc aaccgtctcg cccgaagcct gctccggcga catgtaggcc accgtcccca
     2221 gcaccagtcc cgcccgcgtc gtgttcacgt ccgccacctt cgcgaccccg aagtcgagga
     2281 ttttcacgcg cccctgcgtg gtgacgaaca ggttggccgg cttgatgtcg cggtggacga
     2341 tgcccgcctc gtgcgcgtgc gccagcccgt ccgccgcctg ctcggcgtag gcgatggctt
     2401 cgccgacggg cagcgcgccg cgcgcgacct tctccttcag cgtctcgccg tcgtagcacg
     2461 gcatggcgat gaagagctgg ccgtcggggg tttcgccgac ctcgtagacc gtgcagatgt
     2521 tcgggtggtc gagcgaggcc gcggccctcg cctcctgtag aaaacggcgc ttcagctcgg
     2581 cgtcggcccc gaggtagtcg ggcaggaact tcagggccac gggcctttcg agcttcaggt
     2641 cgcgcgccct gtagaccacc cccatgccgc cctcgccgag cttctcgctc acctcgtagt
     2701 gcgagacgaa ctgacgcggc ggcgcgcccg actcttcgct tagcgaaagg gccgacgcgc
     2761 gcagcgcgcc ttcgaacctc gtcgtgagtc ccggccccgc gtcgctcgcc agcaggctct
     2821 cgacctcgcg gcgcagctcc gcgtcgccgc cgcacgcctc ttcgaggaac gccccgcgcg
     2881 cgcccgcggg cagctccacc gcgtgcccga acagctcgcc gacgcgccgc cagcgcgtgc
     2941 cgctcatcgc ggcggacggc tcggcgtcgc gggtctcgcg tgcgccgctc atgccgtgcg
     3001 tcgtccactg actgcggccc gcgcccgcgc cgatgaagtc gcggacttcc gtcaggaagt
     3061 ggcgccacgc cggttcgctt tcgagcagga cgtggttgtt gctctccagc ggcacgaagc
     3121 gcgcgccggg gacgagcgac gccagaagac gcccctcgtc gaagggcacg cacaggtcgc
     3181 cgcgcccgtg cagcaccagc gtcggcacgt tcacctgtat ggccaggtcg cacacgtcga
     3241 tgacgtggaa cccgttgagg atgcgcgcgg cgccctccgg cgaggaggag acgcgctgca
     3301 attcgttgaa ccagcggagc tgctccggcg tcccttcggg gatgaagagc gtggtgaaga
     3361 cctgccggaa ggcggggttc tcctggcccc agccgacctt caccagttgc agcagaatct
     3421 cggcctcctc gatctgccgt tgcgaagggt tgcggcgcag gcggcccctc gcgtagcccc
     3481 cgtagaggat gaggtggctc accttttcgg ggtggcgcac ggcgtaggcg atggcgatgg
     3541 aggcgccctg cgagatgccg agcatgggga agcgttcgag tcccgcggcc tcgaccacgg
     3601 cttcgaggtc gcgcacccac gcatcgaccg tgaactcttc ggcgtcgcgg tcggagagtc
     3661 cgcagccgcg ctcgtcgtag cggatcagcg tgcgcccccg cgacagctcg cgcagccagt
     3721 ggcgccagac cgggctgcgc cagtcgaatt cgaggtggct gagccagttc gcggccttga
     3781 ccagcggcgg gccggagccg accacggcgt atgcgatgcg cgccccgtcg gcggaggtgc
     3841 agaagcgtat ctcttgctcg tcgtgtccca tgctctagac ggcgggcgcg gagtgccgcg
     3901 gccccggcag ccgactttta ccctcattcg cggcaaaact caacgactcg cgggccgccc
     3961 gaaggcagcg ggcagtcaag acagaagtca gaagatgctt gagaggtaaa gtgacaagcc
     4021 tcctgcttta tcgcttgaag agcggagagg ttcgggactt tatctctcaa gcctcttcgc
     4081 tcttctgctt ttactgcccg ctgcctgctg cctgctgcct gctcttgctt tatcatcgcg
     4141 ggcgatggca ctggtgcgct ttcccgaccc gcgagactcg acgcccgagg gcatcgtcgc
     4201 cgtcggcggc gagccgcgcc cggacttcct gcgcgaagcc taccgccgcg gcatcttccc
     4261 gtggcccgtc gagggctacc cgctcctctg gttcagcccg cccgagcgcg ccgtcctcga
     4321 cttcgcagag ctgcacgtcg cccgcagcct cgcgcgcgag cggcgccgca cgcgtttgcg
     4381 tttcaccatc gaccgcgcct tcgagcgcgt catccgcgcc tgctcgtccg tcgagcgcgc
     4441 gcgcgagcgc gggacgtgga tcacgcccga agtcgtcgca ggctcctgcg acttccaccg
     4501 gcgcggcgac gcgcacagcg tcgaggcgtg ggagggcgag gagctggtcg gcggcgtcta
     4561 cggcgtggac gcgggcggcg ccttcgcggg cgagagcatg ttctacctgc ggcccaacgc
     4621 ctcgaagctc gcgctgctct tcctcatcga ccacctccaa tcgagaggtc tcgactggct
     4681 cgacatacag gtgatgacgc cccacatgga agcgctcggc gcgcggctca tcagccgcga
     4741 cgaattcctc gaccgcctcg cgcgcgagct gcgacgcggg cgcagacttt tctaacgtta
     4801 gctttcgtcc atgcttgagc gtgacgagcg cggcagcttc aactcacgac tcaaccatcc
     4861 acctccgcct ccaactcgcg caggagcgtc gccgtcgtcg ccgccctttg ctcccaccac
     4921 gcggcgaccg cgccgtgctc cgcgcggcgc gtgtgatgac agcgcgacca cgacgcggcc
     4981 tcttccgcga gccagccgaa gacgccgcgc acgtcctgat tgagcagcgc cttgaggacg
     5041 aacttgcgct cgcccgcgcg caggctgagg ccgagcgtgc gcgccccgcg cccgaggtct
     5101 gcccgcgtca ggatcatccc gctccgcgcg ggcgccagca gcgtccgcaa aaaatcttcg
     5161 cccgcgtacg cgcgcgggtc tacggccagc gcgggcgcct cgcgcaggtg cagcggcagc
     5221 tcgtcctgcg cgccgagctt tcctgcttct tccgcgagcg cacacgcgag cagttctccc
     5281 cagcggctgt agaacggcgg cgcgatctcc gagagcttgt cgaggtagac gggcagccac
     5341 gagagcaggt gctcccacag gaaagccccg cgcgcgcgcc gccagcccgc gcgccgcgcc
     5401 gcgtccgctt cgcccgcctc gaactcggag agccgcgcgt agagcgccag catcagcgtc
     5461 aggtggtcgg cctccgcggg cggcgcctgc ccgagcgcgc gccagaagcc cgccacgcgg
     5521 tcgcgcgcct cgccgcccag catcccctcc gcgccgaggt agacggacgc gtacggcgcg
     5581 agctggaaga cgaagacctc cgtgtgctcg ctcgccgcgg gcaggtctcc gagtccgagc
     5641 gccgccgcca gccgcacgac gccgggccgg tcgggcggct ccgcgagcgc cgcgagtgcg
     5701 cgaaagagtt ccatcgaaaa gaagcaggca gtaggcagta ggcagcaggc agtcaaaagc
     5761 aatttcggac tgcgctgctc aacttcgaca tcgacatctg ttcgaactgc ctgctgccta
     5821 ctgcctactg cctgcttctt ccggcaggcg atagacctct ttcgagggtt tgagcgggcg
     5881 caggagccag taggggcggc gcgtgccgtc gggcgagtgc gtgtcggcgg ggcgcgtctg
     5941 cgagagccag tggcggtaga cctcgcgcgc cttcgcggtg tccacgtgga tgtcgccgta
     6001 gctgtccgag gcttcggcct tgcggacgcg cacggcttga tgccaacagt gctggcccga
     6061 gatggggtcg gggtggacgg ggaaggtcag gttctgatgg acgccgacat ccgtccacca
     6121 gatgttgagc gtgtcggggt cggatgattt gtaagggccg acgccgcgct cgcgccgcag
     6181 gccccactcg acgccgcgct ggtcgagcga gacggtggcc atcatctgcc gctcgccctc
     6241 gtccttcagc ttccagcggc ccatgtggtg gctgcacgcg acgatgccgg gcttgatgcc
     6301 ttccgt
//
LOCUS       DBNGOH010000335         3945 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3901126, whole genome shotgun sequence.
ACCESSION   DBNGOH010000335 DBNGOH010000000
VERSION     DBNGOH010000335.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3945)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3945)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3945
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3901126"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..716
                     /locus_tag="AC159P_06525"
     CDS             <1..716
                     /locus_tag="AC159P_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR3695147.1"
                     /translation="LGDVVIEAKHLVKGYGDRLLIDDLDFSLPPGGIVGVIGANGAGK
                     STLFRMIVGEEKPDSGTIRSGETAVPAYVDQARALPANQSIFEVISAGQDLLKVGRRE
                     MPSRAYVASFGFKGAEQQKKVGLLSGGERNRVHLAKLLRSGGNVLLLDEPTNDLDVNT
                     MRALEEALENFAGCAVVISHDRWFLDRVATHILAFEGDSHVEWFEGNYSAYEEDRRAR
                     LGDAAAQPHRIKYRQLTRG"
     gene            766..996
                     /locus_tag="AC159P_06530"
     CDS             766..996
                     /locus_tag="AC159P_06530"
                     /inference="COORDINATES: protein motif:HMM:NF014059.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin family protein"
                     /protein_id="HIR3695148.1"
                     /translation="MTKEVEAVYEEGRLRPLEPLELPEGARLRIVVMTSEETDAQRSV
                     AEALAEIAALPLEGECDHFSGRDHDSVLYPRK"
     gene            1001..1396
                     /locus_tag="AC159P_06535"
     CDS             1001..1396
                     /locus_tag="AC159P_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009786675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HIR3695149.1"
                     /translation="MIFVDTGAWFAAAVPSDPNHPAASRWLAGNNEPLLTTDYVVDET
                     LTLLRARGERTRALLIGDGFFNGRLAEVYALSADDITLAWEVFKRFDDKDWSFTDCTS
                     KVVIERLGIARAFAFDHHFAQFGTVRIVP"
     gene            1443..1721
                     /locus_tag="AC159P_06540"
     CDS             1443..1721
                     /locus_tag="AC159P_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011530871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
                     /protein_id="HIR3695150.1"
                     /translation="MKATWKGAVLAESDETVVVEGNHYFPKESINCENFRESAKQTTC
                     PWKGVASYFDVVVDGEVNKDAAWYYPEPKDAAKEITGRVAFWRGVKVE"
     gene            1752..2069
                     /locus_tag="AC159P_06545"
     CDS             1752..2069
                     /locus_tag="AC159P_06545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695151.1"
                     /translation="MATAEVRVSGLPVRKESIRERLPGAVIEHNEVVVRSALGRSLPL
                     NDHLVGLWNVLRQHRRFLKSLQQEGGKLTCRCMIPKSGAILKPNGAEMLHLLEMELVL
                     EIG"
     gene            2118..2591
                     /locus_tag="AC159P_06550"
     CDS             2118..2591
                     /locus_tag="AC159P_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006918895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HIR3695152.1"
                     /translation="MKKSILTTSFAALALCLGAAGARAQSQAELNREACAQYKVADDE
                     LNRTYALVLKEYKADAAFVESMRAAQRAWLAYRDAHLEAMYPGRDKGLTYGSAHPMCR
                     CYALAEVTRERTAILQRWVTGIEEGDVCAGSIRTRGQERAKARRKPKGRRVVAAL"
     gene            2639..3751
                     /locus_tag="AC159P_06555"
     CDS             2639..3751
                     /locus_tag="AC159P_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019924250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HIR3695153.1"
                     /translation="MIVAVLAALVLCASAPGARVGSGQQRGGAAETPAVKAEWAAALE
                     RVSADSLRGHLSFIASDLLEGRNTPSRGLDIAAEYIAAQFRRAGLEPAGEEGGYFQTA
                     TWGEVAATARRPRQLTPEEAALKVRNVAGLLRGSDASLKDTYVLVSAHYDHVGVRSDA
                     PGDRIFNGANDDGSGTVSVVELASALASLKQRPKRSILFLAFFGEEKGLLGSRLYGRR
                     PLVPLAKTVAQLNLEHLGRTDDSEGPQVGTITMTGFDYTDLGDTLSRAGAATGVKPYK
                     HPRNSDAFFGRSDNQALADLGIPAHTLCTAFIFPDYHGAGDHWDKIDYANLARVARTV
                     ALTLLLVADNPQAPKWNEANPMTAKYVEAWKGLHGK"
BASE COUNT          703 a         1265 c         1402 g          575 t
ORIGIN      
        1 gcctcggcga cgtggtgatc gaggcgaagc acctcgtcaa gggctacggg gatcgcctgc
       61 tgatcgacga cctcgacttc tcgctgccgc cgggcggcat cgtcggcgtg atcggcgcca
      121 acggcgccgg caagagcacg ctgttccgca tgatcgtggg ggaggagaag cccgactcgg
      181 ggacgatccg cagcggcgag accgccgtgc ccgcctacgt ggaccaggcc cgcgcgctgc
      241 ccgcgaatca gagcatcttc gaggtgatca gcgccgggca ggacctgctc aaggtcggcc
      301 gccgcgagat gccctcgcgc gcctacgtgg cctcgttcgg cttcaagggc gccgagcagc
      361 agaagaaggt gggcctcctc tccggcggcg agcgcaaccg cgtgcacctc gcgaagctgc
      421 tgcggagcgg cggcaacgtg ctgctgctcg acgagccgac caacgacctc gacgtgaaca
      481 cgatgcgcgc gctcgaagag gctttggaga acttcgcggg ctgcgccgtc gtcatcagcc
      541 acgaccgctg gttcctcgac cgcgtcgcca cgcacatcct cgccttcgag ggcgacagcc
      601 acgtcgagtg gttcgaaggc aactactccg cctacgagga agaccgccgc gcccgcctcg
      661 gcgacgccgc cgcgcagccg caccgcatca agtaccgcca actgacgcgc ggctgatggc
      721 gagcgcgaac cgtcgggcgt acaatgcacc gtgagggttg agacgatgac gaaagaggta
      781 gaggccgtct acgaagaggg gcgcctgcgc ccgcttgagc cgctcgaact gcccgaaggc
      841 gcgcgactcc gcatcgtcgt catgacgagc gaggagacgg atgcgcagag gtcggtggcg
      901 gaagctctgg ccgagatcgc cgcgctgcct cttgagggcg agtgcgacca cttctcgggc
      961 cgcgaccacg actcggtctt gtacccgcga aagtgacggc atgatcttcg ttgacacagg
     1021 cgcctggttc gccgccgccg taccctcaga ccccaatcat ccagccgctt cacgttggct
     1081 cgcgggcaac aacgaaccac tcctaacgac ggactacgtg gttgacgaga cgctgacgct
     1141 gctccgcgcg cgtggcgaaa gaacccgcgc gctcctcatc ggagacggct tcttcaatgg
     1201 acggctggcc gaagtctatg cactctctgc ggacgacatc accctcgctt gggaggtctt
     1261 caaacgtttc gacgataagg attggagctt cacggactgc acgagcaagg tggtcatcga
     1321 gcgtctcggc atcgcgcggg cgttcgcctt cgaccatcac ttcgcgcagt tcggcacggt
     1381 gcgaatcgtg ccctgacttt tcggggcacc ttcatttcag agacacaggg agagtgaaga
     1441 agatgaaagc gacatggaag ggcgcggtgt tggccgagag cgacgagacg gtggtggtcg
     1501 aggggaacca ctatttcccg aaggaatcga tcaactgcga gaacttccgc gagagcgcga
     1561 agcagacgac gtgcccgtgg aagggcgtgg cgagttactt cgacgtggtg gtggacggcg
     1621 aggtgaacaa ggacgcggcg tggtactacc ccgagccgaa ggacgcggcg aaggagatta
     1681 cgggccgcgt cgccttctgg cgcggcgtga aggtcgagtg agggccggct gtgagcgcgg
     1741 ggtgatgagt catggcgacg gcggaagtgc gagtgagcgg gctgcccgtg cgcaaagaat
     1801 cgatccgcga gaggctgccg ggcgccgtca tcgagcacaa cgaggtcgtc gtgcgttcgg
     1861 cgctgggccg gtcgctgccg ctgaacgacc acctcgtcgg gctttggaat gtgctccggc
     1921 agcatcgcag gtttctcaag agcctccaac aggagggcgg gaagctgacg tgccgttgca
     1981 tgatcccgaa gagcggcgcc atcctgaaac cgaatggggc cgagatgctg cacctgctgg
     2041 aaatggaact cgtgcttgag atcggctgac gggcggcggg cggtgagatt acccgacgac
     2101 gcggagactc gaccgacatg aaaaaatcca tactcacgac gagcttcgcg gcgctcgcgc
     2161 tgtgcctcgg ggctgcgggg gcgcgggcgc agtcgcaggc ggagttgaac cgcgaggcgt
     2221 gcgcgcagta caaggtggcg gacgacgagc tgaaccggac gtacgcgctg gtgctgaagg
     2281 agtacaaggc ggacgccgcc ttcgtcgaga gcatgcgggc ggcgcagcgc gcgtggctgg
     2341 cgtaccgcga cgcacacctc gaagcgatgt atccggggcg cgacaagggg ctgacgtacg
     2401 ggagcgcgca cccgatgtgc cgctgctacg cgctggccga ggtgacgcgc gagcgcaccg
     2461 cgatcttgca acggtgggtc acggggatcg aagagggcga cgtgtgcgcc gggtcgatca
     2521 ggacgcgcgg gcaggagagg gcgaaggcgc gacggaagcc gaaggggcgt cgcgtcgtcg
     2581 ctgctttata gacgaaggaa gcggaggcga aaaacggatg catcggcggc gtgggttgat
     2641 gatagtggcg gtgctcgcgg cgctcgtgct gtgcgcgtcg gctccgggcg cgcgggtcgg
     2701 ctccgggcag cagcgcgggg gcgcggcaga gacgcccgcg gtcaaggcgg agtgggcggc
     2761 ggcgctcgaa cgcgtgtcgg cggactctct gcgcgggcat ctgtcgttca tcgcgtccga
     2821 tttgctggaa gggcgcaaca cgccttcgcg cggcctcgac atcgccgccg agtacatcgc
     2881 cgcgcagttt cgccgcgcgg ggcttgaacc ggcgggcgag gagggcggct acttccagac
     2941 cgcgacgtgg ggcgaggtcg ccgccaccgc gcgccgcccg cgacagttga cgccggaaga
     3001 ggcggcgctg aaagtccgca acgtcgcggg gctgttgcgc ggctccgacg cgtcgctgaa
     3061 ggacacgtac gtcctcgtct cggcgcacta cgaccacgtc ggcgtccgct ccgacgcgcc
     3121 gggcgaccgg attttcaacg gcgcgaacga cgacggcagc ggcacggtct ccgtcgtcga
     3181 gcttgcctcg gcgctcgcct cgctcaagca gcgaccgaaa cggagcatcc tcttcctcgc
     3241 cttcttcggc gaggagaagg gcttgctcgg ctcgcgcctc tacgggcggc gcccgctcgt
     3301 gccgctggcg aagacggtcg cgcagctcaa cctcgaacac ctcgggcgca cagacgacag
     3361 cgaggggccg caggtcggca ccatcacgat gaccggcttc gactacacgg acctcggcga
     3421 cacgctcagc cgcgcgggcg cggccacggg cgtcaagccc tacaagcacc cgcgcaacag
     3481 cgacgccttc ttcgggcgca gcgacaacca ggcgctcgcg gacttaggca tcccggcgca
     3541 cacgctctgc accgccttca tcttccccga ctaccacggc gcgggcgacc actgggacaa
     3601 gatcgactac gccaacctgg cccgcgtcgc gcgcaccgtc gcgctcacgc tgctcctcgt
     3661 cgccgacaac ccgcaggcgc cgaagtggaa cgaggccaac ccgatgacgg cgaagtacgt
     3721 cgaggcgtgg aaggggctgc acggcaagtg agccgcggaa gcggagaata agggcgcggg
     3781 cggcgacggg gcgcccgcgc atttttttgg attcgtcacg ccacgcaacc cgctcgccct
     3841 cataatccgg cgtctcgtca cattcccttt gccgggctga aaccggcggg taaactgccg
     3901 cgtaacatgc gtgccctgca atcttaagag gtgtgccgcc gccgc
//
LOCUS       DBNGOH010000336         2587 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3901315, whole genome shotgun sequence.
ACCESSION   DBNGOH010000336 DBNGOH010000000
VERSION     DBNGOH010000336.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2587)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2587)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2587
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3901315"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1392)
                     /locus_tag="AC159P_06560"
     CDS             complement(<1..1392)
                     /locus_tag="AC159P_06560"
                     /inference="COORDINATES: protein motif:HMM:NF006113.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family peptidase"
                     /protein_id="HIR3695154.1"
                     /translation="MFSFRRSLTNFLFVLTLCVALLAAVLLVNTLRFTSEQIKVEPIQ
                     PVRVDEGGAATRLAQAVRFQTVSYEDAGQIRGEEFLALHKYLEQTFPLAHSTLTRELV
                     GDYSLLYTWKGRDESLKPILLMGHLDVMPVEQQTLAQWEQPPFEGLITEGYIWGRGAL
                     DDKCTVLGIMEAVEMHLSHGFRPQRTIYLAFGHDEEVGGHGGAAKIAGLLRERGVELE
                     YVLDEGSPITDGFFPDVDKPVALVGVADKGYLSLELSVEAEGGHSSMPPPQTAIGVLS
                     AAVSRLEARQVPPSIDGVPRQTLESVGREMPFGRRLVLANLWLFKPLVAHSLAASPST
                     SHGVRTTTAATIIEGGHKENALPGRARAVVNFRILPGDSSERVIAHVADVVNDPRVKV
                     NRFGVSNGEPSAVSDVNAAGYQVIERTLREVFPEVLVAPGLVVGGTDSEHYAALSKNI
                     YRFMPFRVRPEDAK"
     gene            1885..>2587
                     /locus_tag="AC159P_06565"
     CDS             1885..>2587
                     /locus_tag="AC159P_06565"
                     /inference="COORDINATES: protein motif:HMM:NF013698.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HIR3695155.1"
                     /translation="MPHPSGRASAPPRLTNDEAGFAQLSEWLARGSAGPVHACLEATG
                     AYCEALATYLHDAGHAVSRVNPAAITAYAQSHLSRTKTDRVDASLIAGFCSERRPPEW
                     RPPAPEVRELQALVRRLAALVEMRVAEENRLAAGGTVAAVRASVEEHIAYLAEQLKRT
                     EKLIRDHIDQHPGLRRQRELLDSIPGIGEATAAVLLAEVPDIKQDKGARQVAAFAGLV
                     PRERQSGSSIKGRVRL"
BASE COUNT          431 a          866 c          878 g          412 t
ORIGIN      
        1 cttcgcgtcc tccgggcgga cccggaacgg catgaagcgg tagatgttct tcgataacgc
       61 cgcgtagtgc tcggagtccg tcccgccgac gaccagtccg ggcgcgacaa gcacctccgg
      121 gaacacctcc cgcagcgtcc gctcgatgac ctgataaccg gccgcgttga cgtccgagac
      181 ggcggacggt tcgccgttcg agaccccgaa cctgttcacc ttcacgcgcg ggtcgttaac
      241 gacatcggcg acgtgcgcga tgacgcgttc gctgctgtcg ccggggagga tacggaaatt
      301 cacgacggcc cgcgccctgc ccggcagggc gttctccttg tgccccccct cgatgatcgt
      361 cgctgccgtg gtcgtgcgga cgccgtggct cgtgctgggc gacgcggcca agctgtgcgc
      421 gactaggggt ttgaagagcc agaggttggc cagcaccagc ctcctgccga agggcatctc
      481 ccgcccgacg gactcgagcg tctggcgcgg caccccgtcg atgctcgggg ggacttggcg
      541 cgcctcgagc ctgctgacgg ccgcgctgag gacgccgatc gccgtctgcg gcgggggcat
      601 ggaggagtga ccgccctcgg cttcgacgct gagttcgagg ctcaggtagc ccttgtcggc
      661 gaccccgacc agcgccacgg gcttgtcgac atccgggaaa aacccgtcag tgatgggaga
      721 cccctcgtcg aggacgtatt cgagctcgac acccctctcg cgcaagagtc cggcgatctt
      781 ggcggcgcct ccgtggccgc cgacctcctc gtcgtgaccg aacgccagat agatcgtgcg
      841 ctgcggccgg aacccgtggc tcaagtgcat ctcgacggct tccatgatgc cgagcaccgt
      901 acacttgtca tcaagcgcgc ccctgcccca gatgtatccc tcggtgatta gaccctcaaa
      961 cgggggctgc tcccactgag ccagggtctg ctgctcgacg ggcatgacgt ccagatggcc
     1021 catcaggagg atcggcttta agctttcgtc gcgacccttc cacgtgtaga gcaggctgta
     1081 gtcaccgacc aactctctgg tcagcgtcga atgggccaga gggaaagtct gttcgaggta
     1141 cttatgtagg gcgaggaatt cctcgcccct tatctgcccg gcgtcttcat atgagactgt
     1201 ctggaaacgg acagcctggg ccaggcgagt ggcggcgccg ccctcgtcga cacggacggg
     1261 ctggatcggc tcgaccttga tttgttcaga ggtaaagcgg agggtgttga ccagcaggac
     1321 ggcggcgagc agggcgacac acaaggtgag gacaaataaa aaattcgtca gcgacctcct
     1381 gaagctgaac acgtatgacc tcctcggcgg gcgccggtgt ggcactggtc gggaaggagg
     1441 ggccggtcag gggggaagtg tatgggggac gcttccgcgc ggccggtccg cggctgtgta
     1501 gaccccttgg acggggcgaa ctctatgccc gtgcgcgtag ccagtcaagg cctggtcgta
     1561 acaaagagat gataggggag gaaggttttt cggtgacgcc gggcattgta tgttgactga
     1621 agcgggccgc agcgacaagc tctcagccgc accagaggcg ccggtgaggc tgtgcccggc
     1681 atccggccgc cgagcctgcg ggtgagatca agcccccggc gcctcccacc ctgcgcgcgc
     1741 cacacaggcc ggacagtatc tcatggccgc tcactctgag ctgagcctgg attggcaagg
     1801 ctggtcgggc gccgcaccca ccgggagcac cgtcatgaac ctaccgccgc tcggcctcga
     1861 tgtcgctaaa ccgaagttca acgcctgcct catccgtccg ggcgggcgtc tgcgccaccg
     1921 cgtcttacca acgacgaggc cggcttcgct cagctctccg agtggctcgc gaggggcagc
     1981 gccggcccgg ttcacgcctg tctggaggcg accggcgcct actgcgaggc gctcgccacc
     2041 tacctgcacg acgcggggca cgccgtcagc cgcgtcaacc cggcggccat cacggcgtac
     2101 gcgcaaagtc atctctcgcg caccaagacg gatcgggtgg acgcctcgct gatcgccggc
     2161 ttctgctcgg agcggcgccc gcccgagtgg cgcccgcccg cgcccgaagt gcgcgagttg
     2221 caggcactcg tccgccggct cgcagcactc gtcgagatga gggtggccga agagaaccgg
     2281 ctcgcggcgg gcggcaccgt ggccgccgtg cgggcgtcgg tcgaggagca catcgcctac
     2341 ctcgccgagc agctcaagcg caccgagaag ctcatccgcg accacatcga ccagcacccc
     2401 ggcctgaggc ggcagcgcga gttgctcgac tcgatccccg gcatcggcga ggcgactgcc
     2461 gccgtgctgc tggccgaagt gccggacatc aaacaggaca agggtgcccg gcaggtcgcg
     2521 gccttcgccg ggctggtgcc gagggagcgg cagagcggca gctccatcaa ggggcgggtg
     2581 cggctct
//
LOCUS       DBNGOH010000337         6882 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_5000259, whole genome shotgun sequence.
ACCESSION   DBNGOH010000337 DBNGOH010000000
VERSION     DBNGOH010000337.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6882)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6882)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6882
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_5000259"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(149..706)
                     /locus_tag="AC159P_06570"
     CDS             complement(149..706)
                     /locus_tag="AC159P_06570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695156.1"
                     /translation="MQRNTVTRPRARALLFTALFAASALCAPSAFVGAKPSPAPPPAQ
                     DDKKRKEYEKRTALYKTFLDNFRGDAAEQKIAYEAGKEYLKKYSSLKSPSNAAVVDYL
                     RHWTAKYEEAVVEFERWKKSRKGIGEGRAPENSGPGLGENPEGVGTGAGVKLETKPEN
                     APAQPPTKQPDNPPETDKPDGASRW"
     gene            complement(730..2421)
                     /locus_tag="AC159P_06575"
     CDS             complement(730..2421)
                     /locus_tag="AC159P_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045271 - respiratory chain complex I
                     [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048039 - ubiquinone binding [Evidence
                     IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="complex I subunit 4 family protein"
                     /protein_id="HIR3695157.1"
                     /translation="MKLPYLVSIVTWLPAVGAILILLLFKKEQSDAVKKFATAWFGLA
                     FLVSLGLLGYDRAVGGMQFVEDCQWIPVIGARYQMGVDGVAVLLIVLTTLLGVIAAAS
                     SWEYIGKREKEYYVLLLLLQTAVVGVFGAMDLFLFYLFFEVSLVPMYFLIGIWGGERR
                     LYAAIKFFLYTLVGSVVLLLGVIKMYFLTQDSALTGAITQATLDVVTGNAGARAVLDN
                     SLAEAARNGTGTFNIMYLQALGSVLPMGTLQVLLFFAFALGFAIKVPMWPFHTWLPDA
                     HVEAPTAGSVILAGILLKLGTYGFFRFNLPMFPAASADQTYWGGFGVRNVMIVLAIIG
                     IIYGALAAMYFVVKHDGDVKKLVAYSSVSSMGIVILGLFALNPNGLNGAVLHMINHGI
                     YSGALFLLVGIIYERRHTRVVSEFGGLSHVMPGYAAVFLAMAMTAIGLPLLCVFISEF
                     LAMRGAFEANPWWAAWAALGIILNAGYMLWLYQRMFFGNVENPKNRTLPDLNRREWAY
                     MLPLVALSLWIGVYPAPFLRYIERPVNAVVRHIRPDYPMPNAPPRAAEAPERAGK"
     gene            complement(2551..4938)
                     /gene="nuoL"
                     /locus_tag="AC159P_06580"
     CDS             complement(2551..4938)
                     /gene="nuoL"
                     /locus_tag="AC159P_06580"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005141.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HIR3695158.1"
                     /translation="MGEPAGAKRGSLLDVEWSYQLDPLSAVFMLIVTGVGLCIFVFAT
                     GYMHGDPGFYRFFAYLGLFMFSMLVLVMGSNFLMMFVGWEGVGLCSYLLIGYYFDRQE
                     AGDASRKAFITNRIGDFGFALAVFGIIATFGSAQYTAVMEQARAYPIELIGTWGIMSW
                     IALGLFVGACGKSAQIPLYVWLPDAMAGPTPVSALIHAATMVTAGLYMLTRTNVIFQH
                     SQTMMLVVAVVGALTALFAATIGITQNDIKKVLAYSTVSQLGFMFLACGVGAFVIGIF
                     HVMTHAFFKALMFLGAGSVIHGMHHEQDMRRMGNLRKYMPKTYLTFMAGWLAICGVIP
                     FSGFWSKDEILWNAASTTYIPGGWLLWLLATIAATCTAFYMTRLMAMTFWGKERFLEV
                     RAGGQADEAHAAAYDEGKELHAAIDPSVADRPHHGPEDRVGVAHVLETEAVGDAEGVD
                     ARGHDIHDHHDPHEDESHGHAHDDHGHPHGSVIPHESPPSMWGPLAVLAVLAVVGGLV
                     GVGPAFKFLTGTDHPGGRVNIVNWLDPVIWNPATKEFGKEHAAAGEHAKVWTTEPNTT
                     PVFGNEGPFAPRVEKASFTEAAAQETHAAPSDKARSEAGHGDNALPYGDTGFNLAHSV
                     EHALHSHAAAEWVFIVISLVVAGLGMLLGFLFYVWRPHLPGVWAARLGGLYRASYNKY
                     WVDELYGAALTRRTMDAARGVYQIDSKVVDGAVNGAASLTRQASTLTGDVDRLVVDGA
                     VNGLAGFVKLLVSPLFRAAQTGLTANYALVMILGLVATVALYFGRDILAALLGNQ"
     gene            complement(5346..5741)
                     /gene="nuoK"
                     /locus_tag="AC159P_06585"
     CDS             complement(5346..5741)
                     /gene="nuoK"
                     /locus_tag="AC159P_06585"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017327686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HIR3695159.1"
                     /translation="MTLFLSTAFFLLQESKLERITQGVQYPDLTKFLVIGALLFTIGV
                     AGVLTRRNIIVIFMSIELILNAANLNFIAFARYHEATGGMSAIAGQVFTVFVIVVAAA
                     EAAIGLGIVIALYRNRETIFIDKIDLLKW"
     gene            complement(5834..6511)
                     /locus_tag="AC159P_06590"
     CDS             complement(5834..6511)
                     /locus_tag="AC159P_06590"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012709.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HIR3695160.1"
                     /translation="MVLSGWESVFFWVFAIAALVAGLLTIMAHNAVHSALFLISTLVS
                     VAALFVLLGAEFIAGVQILVYVGGVMVLFLFVIMLVNVSAEERGREELFNRPGQVGAA
                     LVFSALLVFGLIYAINTSASRFQPRAGGETPAERAANYEEARREEERNLRPAATRTSK
                     ISQDTQQVGASLYRYGALPFEIASVLLLVAIVGSVLLARTMKQEAAAEDLAPEVAAQE
                     VRREAEL"
     gene            complement(6594..>6882)
                     /locus_tag="AC159P_06595"
     CDS             complement(6594..>6882)
                     /locus_tag="AC159P_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014098966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HIR3695161.1"
                     /translation="IACNLCALACPEDCIDVIAMDIEMTVGGKARKKKVLDEFIFDTS
                     RCMFCALCQEACPTACLELTQDFELATYSRAGFVWNREMLEQGREVKKFEA"
BASE COUNT         1246 a         2205 c         2297 g         1134 t
ORIGIN      
        1 gtccccgaaa gataaaagtt gccgcgagag aggttcgcct tgacctggga aacgacttcg
       61 aggccgtgtt cgcgcaacgc ccgcgagatg tcctgcgggt cgttcacagg gttgctgaac
      121 ggcgcgacat cgtaagcgct gttcccgttc accaacgcga cgcgccgtcc ggcttatccg
      181 tttccggagg gttgtcgggt tgtttggtgg gaggttgagc gggagcgttt tccggcttgg
      241 tttccagttt gacccctgcc ccggtgccta cgccttccgg gttctcgcct aaaccggggc
      301 ctgaattctc cggggcgcgc ccctctccta tgcccttccg agattttttc cagcgttcga
      361 actcgacgac cgcctcctcg tacttggctg tccagtgccg aaggtagtcc acgaccgccg
      421 cgttgctggg gcttttcagg ctgctatatt ttttcagata ctccttgccg gcctcgtagg
      481 cgatcttctg ctccgccgcg tcgcctctga agttgtcgag gaatgttttg tagagcgccg
      541 tgcgcttttc gtattccttg cgctttttgt cgtcctgcgc cggaggaggc gcgggcgaag
      601 gtttcgcccc cacgaacgcc gacggtgcgc agagcgccga ggccgcgaag agggcggtaa
      661 acagaagggc gcgcgcgcgc ggacgggtga cggtgttgcg ctgcatgatt ccacctcgct
      721 gtgacatctt cacttccctg cccgctccgg cgcctcggcg gcgcgcggcg gcgcgttcgg
      781 catggggtag tctgggcgga tgtggcggac gacggcgttg accgggcgct cgatgtagcg
      841 gaggaagggc gcggggtaga cgccgatcca gagcgagagc gcgacgagcg ggagcatgta
      901 ggcccactcg cgccggttga ggtcgggcag cgtccggttc ttcgggttct cgacgttgcc
      961 gaagaacatc cgctggtaga gccagagcat gtagcccgcg ttcaggatga tgcccagggc
     1021 cgcccacgcc gcccaccacg ggttcgcctc gaacgcgccg cgcatcgcca gaaattccga
     1081 gatgaagacg cacaagagcg gcagcccgat ggccgtcatc gccatcgcga ggaacacggc
     1141 ggcgtagccc ggcatgacgt gcgagaggcc gccgaactcc gagacgacgc gcgtgtgccg
     1201 ccgctcgtag atgatgccga cgagcaggaa cagcgcgccc gagtagatgc cgtggttgat
     1261 catgtggagc acggcgccgt tcagcccgtt cgggttcagg gcgaacagcc cgaggatgac
     1321 gatgcccatc gaggagacgg acgagtaggc gacgagtttt ttcacgtcgc cgtcgtgctt
     1381 gacgacgaag tacatcgcgg cgagcgcgcc gtagatgatg ccgatgatgg ccaacacgat
     1441 catcacgttg cggacgccga agccgcccca gtaggtctgg tcggcggagg ccgccgggaa
     1501 catcggcagg ttgaagcgga agaagccgta ggtgccgagt ttcagcagga tgccggcgag
     1561 gatgacggag ccggcggtcg gcgcctcgac gtgcgcgtcc ggcagccacg tgtggaacgg
     1621 ccacatcggc accttgatgg cgaagccgag cgcgaaggcg aagaagagca gcacttgcag
     1681 ggtgcccatc ggcaacaccg agccgagcgc ctgcaggtac atgatgttga aggtgccggt
     1741 gccgttgcgc gccgcctcgg ccagactgtt gtcgagcacg gcgcgtgcgc ccgcgttccc
     1801 cgtcaccacg tcgagcgtcg cctgcgtgat ggcgcccgtc agcgccgagt cctgcgtcag
     1861 gaagtacatc ttgatgacgc cgagcagcag gacgaccgag ccgaccagcg tgtagaggaa
     1921 gaacttgatc gccgcgtaga gcctgcgctc gccgccccag atgccgatga ggaagtacat
     1981 cggcacgagc gagacctcga agaagaggta gaagaggaac aggtccatcg cgccgaagac
     2041 gccgaccacc gccgtctgca acagcagcag cagcacgtag tattccttct cgcgcttccc
     2101 gatgtactcc cacgaagagg cggcggcgat gacgccgagc agcgtcgtca gcacgatcag
     2161 cagcacggcc acgccgtcca cgcccatctg gtatcgcgcg ccgatcaccg gtatccactg
     2221 gcagtcctcg acgaactgca tcccgcccac cgcgcggtcg tagccgagca ggcccagcga
     2281 gacgaggaac gcgaggccga accacgcggt cgcgaacttc ttgacggcgt cggactgctc
     2341 cttcttgaac aggagcagga tgagaatagc gcccaccgcc gggagccacg tcacgatgga
     2401 gaccagataa gggagtttca cgattcctcc gaaaacagaa gtcagaagtc ggaagtcaga
     2461 agaaaagctt tcgtcaacga atcgtccgag tatcagcgtc agcgcccttg atgcggagca
     2521 ttccgacttc cgacttccga cttccgactt tcactggttg ccgagcaagg ccgccaggat
     2581 gtctctgccg aagtagagcg cgaccgtggc gacgaggccg agtatcatga cgagggcgta
     2641 gttggcggtc aggcccgtct gcgccgcgcg gaagagcggc gagacgagga gcttgacgaa
     2701 gcccgcgagg ccgttgaccg cgccgtccac gacgaggcga tccacgtcgc ccgtcagcgt
     2761 gctcgcctgc cgcgtcaacg aagccgcgcc gttgaccgcg ccgtccacca ccttcgagtc
     2821 gatctggtag acgccgcgcg ccgcgtccat cgtccgccgc gtcagagctg ccccgtagag
     2881 ttcgtccacc cagtatttgt tgtagctcgc gcggtacagc ccgccgagcc gcgcggccca
     2941 cacgccgggc aggtgcggcc tccacacgta gaacaaaaag ccgagcagca tgccgagtcc
     3001 cgcgacgacg agcgagatga cgatgaagac ccactccgcc gccgcgtgcg agtggagcgc
     3061 gtgctcgacc gagtgcgcga ggttgaagcc ggtgtcgccg taaggcagcg cgttgtcgcc
     3121 gtgcccggcc tcgctccgcg ccttgtcgct cggtgcggcg tgcgtctcct gcgcagcggc
     3181 ttcggtgaac gaggctttct cgacgcgcgg cgcgaacggg ccttcgttgc cgaagacggg
     3241 ggtcgtgttc ggttccgtcg tccagacttt cgcgtgctcg cccgcggcgg cgtgctcttt
     3301 gccgaactcc ttcgtcgcgg ggttccagat gacggggtcg agccagttca cgatgttgac
     3361 cctgccgccg gggtgatccg tccccgtgag gaacttgaag gccgggccga cgccgacgag
     3421 tccgccgacc accgcgagca cggcgagcac ggccagcggc ccccacatcg acggcggcga
     3481 ctcgtgcggg atgaccgagc cgtgcgggtg cccatgatcg tcgtgcgcgt gcccgtgcga
     3541 ttcgtcttcg tgcgggtcgt ggtggtcgtg tatgtcgtgg ccgcgcgcgt ccacgccttc
     3601 ggcgtcgccg acggcctcgg tctcaagcac gtgcgcgacg ccgacgcgat cttccggccc
     3661 gtggtgcggg cgatccgcga ccgaaggatc aatcgccgcg tgtagctctt tgccttcgtc
     3721 gtaggcggcg gcgtgcgcct cgtccgcctg cccgcccgcg cgcacttcga ggaagcgctc
     3781 cttgccccag aaggtcatcg ccatcaggcg cgtcatgtag aaggcggtgc atgtcgcggc
     3841 gatggtcgcc agcagccaga gcagccagcc gccggggatg taggtggtcg aggcggcgtt
     3901 ccacagaatc tcgtccttcg accagaagcc cgagaacggg atgacgccgc agatggcgag
     3961 ccagcccgcc atgaacgtca ggtaggtctt cggcatgtac ttccgcaggt tccccatcct
     4021 tcgcatgtcc tgctcgtggt gcatgccgtg gatgaccgaa cccgcgccga ggaacatcag
     4081 cgccttgaag aaggcgtgcg tcatgacgtg gaagatgccg atgacgaagg cgccgacgcc
     4141 gcacgcgagg aacatgaagc cgagctgcga gaccgtggaa taagccaaga ccttcttgat
     4201 gtcgttctgc gtgatgccga tggtcgcggc gaagagcgcg gtcaaagcgc cgacgaccgc
     4261 cacgacgagc atcatcgtct gcgagtgctg gaagatgacg ttcgtgcgcg tcagcatgta
     4321 gaggcccgcc gtcaccatcg tcgcggcgtg gatgagcgcc gagaccggcg tcggcccggc
     4381 catcgcgtcg ggcagccaga cgtagagcgg tatctgcgcg ctcttgccgc acgcgccgac
     4441 gaacagcccg agcgcgatcc acgacatgat gccccacgtg ccgatcagct cgatggggta
     4501 ggcgcgcgcc tgctccatca ccgccgtgta ctgcgccgag ccgaaggtgg cgatgatgcc
     4561 gaagaccgcg agcgcgaagc cgaagtcgcc gatgcggttc gtgatgaacg ccttgcgcga
     4621 cgcgtcgccc gcctcctggc ggtcgaagta gtagccgatg aggaggtacg agcagaggcc
     4681 gacgccctcc cacccgacga acatcatcag gaagttcgac cccatcacca gcaccagcat
     4741 cgagaacatg aagaggccga ggtaggcgaa gaagcggtag aagccggggt cgccgtgcat
     4801 gtagcccgtg gcgaagacga agatgcacag accgacgccc gtcacgatga gcatgaagac
     4861 ggcggacaga gggtcgagct gataactcca ctccacgtcg agcagcgagc cgcgcttcgc
     4921 gcccgccggc tcgcccacgg tcgtcgtggc cgcgccgcgc agcccctcgc cgtgctgcgc
     4981 cgggttgacg tcctgcgact gcgcgctcgc ctgcgcgcgc tcggtctcgc cttgcgtgat
     5041 ctcgacggcg ccgcccggaa tccacgtgaa gaccatgtcg gtcgtcacgt aaggcttcgg
     5101 ccagatggcg tgctcgccga agccgtagga gacgaccgcg cccacgctca gcaggaacgc
     5161 gagcgccacc gaccccacgg ccaccgcgcc gatcagcctt tcggagaagc ggaaccggcg
     5221 accgagcaag ccgttgatcg cggcgcccgc gagcggcagc agggggacga gccagatgag
     5281 tctatgcata ggattgccgt aaacggtaaa tggtcaatgg aagcagacca tttactattt
     5341 accattcacc actttaagag gtcgatctta tcgatgaaga tcgtctcgcg gttgcggtag
     5401 agcgcgatga cgatgccgag gccgatggcg gcctcagccg ccgcgacgac gatgacgaag
     5461 acggtgaaga cctgcccggc gatggcgctc atcccgcccg tcgcctcgtg gtagcgcgcg
     5521 aaggcgatga agttcaggtt ggccgcgttc aggatcagct cgatcgacat gaagatgacg
     5581 atgatgttgc gccgcgtcag cacgcccgcc acgccgatgg tgaagagcag cgccccgatc
     5641 accaggaact tcgtgaggtc ggggtactgg acgccctgcg tgatgcgttc gagcttcgac
     5701 tcttgcagca ggaagaacgc cgtcgagaga aacagggtca taaggttttc caggagtcag
     5761 aagtcaggag ccggaagtca gaataaaagc ggtcgccgcc ttcgtccctt ccgactgctg
     5821 acttctgtct ccttcaaagt tcggcctccc gtctgacttc ttgcgccgcg acttcgggcg
     5881 cgaggtcttc cgccgcggcc tcctgcttca tggtgcgcgc cagaagcacc gagccgacga
     5941 tggcgacgag caggaggacg ctggcgatct cgaagggcag agcgccgtag cggtagaggc
     6001 tggcgccgac ctgctgcgtg tcctgcgaaa tcttcgaggt gcgggtcgcc gcgggccgca
     6061 ggttgcgctc ctcttcgcgg cgggcctctt cgtagttggc ggcgcgctcc gccggggtct
     6121 cgccgccggc ccgcggctgg aacctgctcg ccgaggtgtt gatggcgtag atgaggccga
     6181 agacgagcag cgccgagaag accagcgccg cgccgacctg ccccggtcgg ttgaacaact
     6241 cctcgcgccc gcgctcctcg gcgctcacgt tgacgagcat gatgacgaac aggaagagca
     6301 ccatcacgcc gccgacgtag accagaatct gcacgcccgc gatgaactcc gcgccgagca
     6361 ggacgaagag cgccgccacc gacaccagcg tcgagatgag gaacagcgcc gagtgcacgg
     6421 cgttgtgcgc catgatcgtc agcaggcccg ccaccagcgc cgcgatggcg aacacccaga
     6481 agaagacgct ttcccagccc gataagacca taagattcag tggtcagtgg tcagtggtcg
     6541 gtggtcagtt aaagatcggt ttttcactga ccaccgacca ctgacctccg ctcctatgct
     6601 tcgaacttct tcacctcgcg gccctgttcg agcatctcgc ggttccagac gaagccggcg
     6661 cgcgagtagg tggcgagttc gaagtcctgc gtcagttcga ggcaggccgt ggggcacgcc
     6721 tcctgacaga gcgcgcagaa catgcagcgc gaggtgtcga agatgaattc gtcgaggact
     6781 ttcttcttgc gcgccttgcc gccgaccgtc atctcgatgt ccatcgcgat cacgtcgatg
     6841 cagtcctcgg ggcaggccag cgcgcagagg ttgcaggcga tg
//
LOCUS       DBNGOH010000338         3875 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_784459, whole genome shotgun sequence.
ACCESSION   DBNGOH010000338 DBNGOH010000000
VERSION     DBNGOH010000338.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3875)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3875)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3875
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_784459"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2372)
                     /locus_tag="AC159P_06600"
     CDS             complement(<1..2372)
                     /locus_tag="AC159P_06600"
                     /inference="COORDINATES: protein motif:HMM:NF012801.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR3695162.1"
                     /translation="MRREIAVSGLAVTGIVIRLAAACVLALACAATAAARQSSSLSGR
                     VTDARGAAVPGATVTLYARGTAAPRLATATDEAGAYRFERLAPGEYLVEAGARGFARG
                     APREVTVARGAAGAATLDIALEVAGVSAEVVVTASDSPQPVDEVSKAVHVIGREELDE
                     RDEFSIPEALRTVPGLRVRRLGGPGSLTTIRTRGLRNEDTAVLVDGLRLRDPSAPQGD
                     ASSLIGDLTTTDVSRVEVLRGSGSSLYGTNAVGGVINVITDEGGGPLRGNLLAEGGSL
                     GLFRGRAQVAGGAGAADRLAYSAGLSHLNVARGIDGDDAARNTSAQGRVTLRLAPSVT
                     LSGRAYFADSFAQTNHDPQPVGAQTGDITDAVPLSRGELRRFESGTPINQLNLGAANF
                     IPSTNNPDARQRGRFFSGALVFTHRLGEDAAYAVSYQGLDITRANFDGAAGTRFDFDG
                     RTHTLNARADFRVGSSNHVTAGYEFESEDFFNRLFFGDPSLDSGAGATERSHALFVQD
                     QLRLLGDRLQLSAAFRAQFFRLDEARFEPAGSQLFEGPFDAPPAAYTGDGSIAYLFRS
                     TATKLRAHVGNGYRAPSLFERFGASFFFGSFFPFGNPDLRPERSIAFDAGLDQSLADN
                     RLRLAATYFYTRLQETIVFDIPYRNAGGGLARGVELSVEAAPTRATDLFVSYTHTNSD
                     QRTPQTGGIISTFVVPDHQFSAVLTQRLGQRFSLNFDLAASSTHLAPVFDPVTFTSRV
                     YRFDRLFKADLGASYRLPLTDARSLRLFALVENLFDRDYYEGGFRTPGVGA"
     regulatory      complement(2856..3064)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(3256..>3875)
                     /locus_tag="AC159P_06605"
     CDS             complement(3256..>3875)
                     /locus_tag="AC159P_06605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695163.1"
                     /translation="MRMLALSCLSLVLAGAARAQSPAAPPDVAVEKYHWSKERIGWEA
                     DPFGGPVEGFDDIRRRLADQRRVERARATGNIGEANKVEREMRAEQVLKSRPTTPPRY
                     VFNYKVSLRNTGAKAIREIDWDYVFYDVTSGQELGRRQFTGVEKIDPGKRKELSFLVH
                     SPPTQSISVYSLGRKEREGLREQVVVVRVLYEDGTVWQAPAPPAP"
BASE COUNT          587 a         1217 c         1489 g          582 t
ORIGIN      
        1 gcgccgacgc cgggcgtgcg gaagccgcct tcgtagtagt cgcggtcgaa gaggttctcg
       61 acgagcgcga agagccgcag cgagcgcgcg tccgtgagcg ggagccggta gctcgcgccg
      121 aggtcggcct tgaacagcct gtcgaagcgg tagacgcggc tcgtgaaggt gacggggtcg
      181 aagacgggcg cgagatgcgt gctcgacgcc gcgaggtcga agttgaggga gaagcgttgg
      241 ccgaggcgct gcgtgaggac ggcggagaac tggtggtcgg ggacgacgaa ggtgctgatg
      301 atgcctccgg tctgcggcgt gcgctggtcg ctgttggtgt gcgtgtagga gacgaagagg
      361 tcggtcgccc tggtcggcgc ggcctcgacg ctcagctcga cgccgcgcgc cagtccgccg
      421 cccgcgttgc ggtaggggat gtcgaagacg atggtctcct gaagccgcgt gtagaagtag
      481 gtcgccgcga ggcgcaggcg gttgtctgct agcgactggt cgaggcccgc gtcgaaggcg
      541 atggagcgct cgggccgcaa gtcggggttg ccgaagggga agaagctgcc gaagaagaag
      601 gaggcgccga agcgctcgaa gagagagggg gcgcggtagc cgttgccgac gtgcgcgcgc
      661 agcttggtcg cggtcgagcg gaagaggtag gcgatggagc cgtcgccggt gtaggccgcg
      721 ggcggcgcgt cgaacgggcc ttcgaagagt tggctgcccg cgggttcgaa gcgcgcctcg
      781 tcgaggcgga agaactgcgc gcggaaggcg gcggagagtt gcaggcggtc gccgagcaga
      841 cgcagttggt cttgaacgaa gagggcgtgg ctgcgctcgg tcgcgccggc gccggagtcg
      901 agcgaggggt cgccgaagaa gaggcggttg aagaagtcct cgctctcgaa ctcgtagccg
      961 gcggtgacgt ggttcgagct gccgacgcgg aagtcggcgc gcgcgttgag cgtgtgcgtg
     1021 cgaccgtcga agtcgaagcg cgtgccggcg gcgccgtcga agttggcgcg cgtgatgtcg
     1081 agaccttgat aggagacggc gtaggcggcg tcttcgccga ggcggtgcgt gaagacgagc
     1141 gcgccggaga agaagcggcc ccgctggcgc gcgtccgggt tgttggtcga ggggatgaag
     1201 ttggccgcgc cgaggttgag ttgattgatg ggagtgccgg actcgaagcg tcgcagctcg
     1261 ccgcgcgaga gcgggacggc gtcggtgatg tcgcccgtct gcgcgccgac gggctgcggg
     1321 tcgtggttcg tctgcgcgaa cgaatcggcg aagtaggcgc ggcccgagag cgtgacggag
     1381 ggcgcgagcc gcagggtgac gcgcccctgc gcgctcgtgt tgcgggcggc gtcgtcgccg
     1441 tcgatgccgc gcgcgacgtt gaggtgcgag aggcccgcgc tgtaggcgag gcgatctgcc
     1501 gcgcctgcgc cgcccgcgac ctgcgcgcga ccgcggaaga gtccgagcga gccgccttcg
     1561 gccaacaggt tgccgcgcaa ggggccgccg ccctcgtccg tgatgacgtt gatgacgccg
     1621 ccgacggcgt tcgtgccgta gagcgaagag ccggagccgc gcaagacttc gacgcggctc
     1681 acgtcggtcg tcgtcaaatc gccgatgagc gaagaggcgt cgccctgcgg cgcagagggg
     1741 tcgcgcaggc gcaggccatc gacgaggacg gcggtgtcct cgttgcgcag gccgcgcgtc
     1801 ctgatggtgg tgagcgagcc ggggccgccg agccgccgca cgcgcaggcc cggcacggtg
     1861 cgcagcgcct cggggatgga gaactcgtcg cgctcgtcaa gctcctcgcg cccgatgacg
     1921 tggacggcct tcgagacttc gtccacgggc tgcggcgagt ccgaggcggt gacgacgacc
     1981 tccgcgctca cgcccgcgac ctcgagcgcg atgtcgagcg tggccgcgcc cgccgcgccg
     2041 cgcgcgactg tgacctcgcg cggcgcgccg cgcgcgaacc cgcgcgcccc tgcttcgacg
     2101 agatactcgc ccggcgcgag gcgctcgaag cggtacgcgc ccgcctcgtc ggtcgcggtg
     2161 gcgaggcgcg gcgccgccgt cccgcgcgcg tagagcgtga cggtcgcgcc cggaacggct
     2221 gcgccccgcg cgtccgtcac acgcccgctg agcgaggatg actgccgcgc cgcggcggtc
     2281 gcggcgcacg cgagcgcgag cacgcacgcg gcggcgagcc tgatgacgat tcctgtcacg
     2341 gcgagtccgg aaacggcgat ctctcttctc atgcttcacc acacctttca agcaaagacg
     2401 agggacgcgc ccgccgacgg gatggtgagc ggtgagcagt gagcagtgag cagtaaaaac
     2461 aggagccgga agaagcgaga gtctttggac ttcacctctc aagcatcttc cctcttctgc
     2521 ctccaactgc tcaccgctta ctgctcactg ctcacttgag cgaagcgaaa gagcgacgca
     2581 agtgtccgtc agccgaaact gaaaacgccc tcggtcttcc gcgttggaag accgagggcg
     2641 tgccggatgt gcgctcgaaa aatcgggaag gccgccggcg cgcttccaag tgcggcgcgc
     2701 ggtgcgttcc ggattccgtg cccgacgcca ctcgccctgt cccggagcgt aagcggtcgc
     2761 ctccgtcagc aaaagccgtc aatcgtaaac cgtgaaccgt gaccagagga gagctgttct
     2821 tctctggtca cggtttaccg tttacggttc gcggcgtttg acgcaggcaa ggtttcctga
     2881 ctcccggctc ggacgcggcg cctcttacgg gccttccccg tccctttagg acgagtggcc
     2941 tctcgcgggt gcgagatgaa ggaggcgtcg cggattcgcc ggtacacagt ggcgggaacc
     3001 gtgcgggctt tgcacccggc ttcccttgga agcccgcgcg tgcggcgggc ttctacacct
     3061 gcgacggttc ggcccggccc cgcgccatcg cgcgggcggg ccgaattagc gatcaagtat
     3121 tcggaaggca aagagctgtt cgcgcctgtc agtcggctga cggctggccg gtcggccgcg
     3181 ggcgtcgagg tgagacacgc taacactggc gcacgccagc gtcaaggaag gcttcgcggc
     3241 cgcgccctca aaggcttacg gcgcgggcgg ggcgggcgcc tgccagacgg tgccgtcttc
     3301 gtagaggacg cggacgacga cgacctgctc gcgcagcccc tcgcgctcct tcctgccgag
     3361 cgagtagacg ctgatgctct gcgtcggggg cgagtggacg aggaaggaga gctccttgcg
     3421 cttgcccggg tcaatcttct cgacgccggt gaactgccgg cggccgagtt cctgcccgct
     3481 cgtcacgtcg tagaagacgt agtcccagtc aatctccctg atggccttcg cgcccgtgtt
     3541 ccggagggag actttgtagt tgaagacgta gcggggcggg gtcgtggggc gcgacttgag
     3601 gacctgctcg gcgcgcatct cgcgctcgac cttgttggcc tcgccgatgt tgccggtggc
     3661 gcgcgcgcgc tcgacgcggc gctggtcggc gaggcggcgg cggatgtcgt cgaagccctc
     3721 gacggggccg ccgaaggggt cggcctccca gccgatgcgc tccttcgacc agtggtactt
     3781 ctcgaccgcc acgtcgggcg gcgcggcggg cgactgcgcg cgggccgccc cggccagcac
     3841 gagggacagg caactgagag ccagcatcct catcg
//
LOCUS       DBNGOH010000339         5697 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_601676, whole genome shotgun sequence.
ACCESSION   DBNGOH010000339 DBNGOH010000000
VERSION     DBNGOH010000339.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5697)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5697)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5697
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_601676"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..624)
                     /locus_tag="AC159P_06610"
     CDS             complement(<1..624)
                     /locus_tag="AC159P_06610"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR3695164.1"
                     /translation="MTPELSVIFVNWNGGELLRRAVECVERFAPSVPFEIIVVDNAST
                     DGSREWLRSLGARVRLVENEENLGFSKANNQGIRASRAPLVFLLNTDAEVRAGTIDRL
                     IETMRADERAGACAPRLVNPDGTLQPSVWRNPPTAWETLLSGLKLYRLIPRRARGRLL
                     LGTFWDHAERRRVPMLPGTALLVRRRTIEDVGALDEGFHMYFEDVEWS"
     gene            complement(714..1706)
                     /locus_tag="AC159P_06615"
     CDS             complement(714..1706)
                     /locus_tag="AC159P_06615"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR3695165.1"
                     /translation="MKFSVAMCTYNGARFLREQLDSIAAQTRPPDELVVCDDRSTDGT
                     TDLVAAFAAAARFPVRLHVNTENLGSTKNFDRAVSLCTGDLIALADQDDVWLPRKLEL
                     SEGALAASPRAGLVFTDAEVVDERLAPLGFTLWQRINFSEEEKALIRRGRGFDVLLEH
                     NVVTGATMAFRAELRDLVLPIPTEGRLIHDGWIALLAAAVSDLAFVELPLVQYRQHEA
                     QQLGAVKGGEFEHRRATFAEVLEAAQATRGADYLRELNWLEPFCARLAEQGGAYRSRA
                     GVVRLNGRLTHLRARASLPRGRLARVPIVLRELLTMRYHRHSRGVLSFAKDLFN"
     gene            complement(1758..2768)
                     /locus_tag="AC159P_06620"
     CDS             complement(1758..2768)
                     /locus_tag="AC159P_06620"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008480044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0019673 - GDP-mannose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0042351 - 'de novo' GDP-L-fucose
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HIR3695166.1"
                     /translation="MKKALITGITGQDGSYLAEHLLGLGYEVHGLVRRVALEDPERRF
                     GRIGHLLDRVELHPASLESYASIFHIFSRHQFDECYHLAAQSFVAESFADGFSTMNTN
                     INGTHYVLAAIRELRPACRFYFAGSSEMFGKVREMPQRETTPFHPRSPYGISKVAGFD
                     LTRNYREAYGMFCASGILFNHEGPRRGFEFVTRKITSTVARIKAGLATELRLGNLDAK
                     RDWGHAKDYVRAMHLMLQQEEPDDFVVATGETHTVREFCERAFAEVGLDWEQYVRVDE
                     RYYRPAEVDLLVGDSAKARAVLGWEPQYTFAALVSEMVREDLRALARPAARAEELAAG
                     GD"
     gene            complement(2812..3840)
                     /locus_tag="AC159P_06625"
     CDS             complement(2812..3840)
                     /locus_tag="AC159P_06625"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR3695167.1"
                     /translation="MPYGTGQPLVSVVVPTYNYGRFIGETLDSLRAQTYPDWECVVVD
                     DGSTDDTEEIVARVAAADPRVRYLRQRNRRQAVAKNKGLADARGVYVQFLDADDLLEP
                     RKFERQVEYLERHPEVDLVYGDMLYFDADDPSRRTRRLEAEAGGAWMPEVSGRGSEVL
                     VPLLRNNIMVINSPLLRRGVVADVGPFDERLPPVEDWDYWIRCAIAGKRFRFVEQEGT
                     LALVRSHPGSSSRDNARMYGAMSLMRRKLEALLPDGELRRLNREMHAAERGAFGIEQV
                     RGGRPLKAVGSFVRAAAAGRGLKWRVRWAACALAAPFVSESQIRALSAASPLHAVAGA
                     GRGKSRRV"
     gene            complement(4031..4990)
                     /locus_tag="AC159P_06630"
     CDS             complement(4031..4990)
                     /locus_tag="AC159P_06630"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR3695168.1"
                     /translation="MTPKVSVLMAVYNGERYLREAIDSILGQTFADFEFIIIDDASTD
                     RSFEIAGGYADPRIRLVRNERNLGLTLSLNRGLDLARGEYVARMDCDDLSLPERLAKQ
                     VAFMEANPDVGACSTWAFDIDSAGNVIGKRELPVGEQLDNFYWRRSLIHPAAMFRFDR
                     SAGPRYDPAFRYAQDYDLWFRIRAERRLCNLPEHLLLYRVHEESITGSRFEAQLRACY
                     DVFCRNTGTRAISFEAYLALTERSWALHPLRRAFAMMRLARRLRTPYRVYFRDDLRYA
                     RVWMYSRQIYNAVARTRTYRVARDVLKRIARAGSRATGIAGVH"
     gene            complement(4987..>5697)
                     /locus_tag="AC159P_06635"
     CDS             complement(4987..>5697)
                     /locus_tag="AC159P_06635"
                     /inference="COORDINATES: protein motif:HMM:NF012936.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0070981 - L-asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HIR3695169.1"
                     /translation="WEMERGGHPRVAVALLWRALRLSSALGLRAPEAWGRITRGAGLR
                     RSYASVARAHAKQKEVFGDEELALLGLDGGHEAAAAEGGGTGTVDDAMRMDLCDYMPG
                     DILVKIDRASMAHGLELRAPFLDVEFASFCISLPSRLKITPREEKWILRRSYGASWPE
                     SVRAREKCGFGAPVNRWLARPDVAALKVRILDDPRHALFSFVPFAASREFAARDNYQT
                     WALLVLGLWLEQGGEASL"
BASE COUNT          896 a         1980 c         1932 g          889 t
ORIGIN      
        1 gctccactcc acgtcctcga agtacatgtg gaagccctcg tccagagcgc ccacgtcttc
       61 gattgtccgc cggcggacga gcagcgcggt gcccggcagc atcgggacgc ggcgccgctc
      121 cgcgtgatcc cagaaggtgc cgagcaggag gcgcccgcgc gcccgccgcg ggatcagacg
      181 gtagagcttg aggcccgaca gcagcgtctc ccacgcggtc ggcgggttgc gccacacgct
      241 cggctggagc gtgccgtccg ggttgacgag ccgcggcgcg cacgcccccg cgcgctcgtc
      301 ggcgcgcatc gtctcgatca acctgtcgat cgtgccggcg cgcacctccg cgtccgtgtt
      361 gaggaggaag acgaggggcg cgcggctcgc cctgatgccc tggttgttgg ccttgctgaa
      421 gcccagattc tcttcgttct cgacgagcct gacgcgcgcc ccgagcgagc gcagccactc
      481 gcggctgccg tcggtcgagg cgttgtccac gacgatgatc tcgaacggca ccgacggcgc
      541 gaaccgctcg acgcactcga cggcgcgccg cagcagctcg ccgccgttcc agttgacgaa
      601 gatgaccgac agctcgggcg tcataattgg cggctggagt ttagagactg gagactagcg
      661 gctggtaaga aagagttgtc ttttaccagc ctctaacctc aagcctcaag cccctagttg
      721 aacaagtcct tggcgaagct caggacgccg cgcgagtgcc ggtggtagcg cattgtcagc
      781 agctccctga gcacaatcgg cacgcgcgcc agcctgcctc ggggcagact ggcgcgcgcc
      841 cgcaggtgcg ttaacctgcc gttcaatctt accacgcccg cgcgcgaacg gtacgcgccg
      901 ccctgctcgg cgaggcgcgc gcagaacggt tcgagccagt tcagctcgcg caggtaatcc
      961 gcgccgcgcg tggcttgcgc cgcctccaga acttcggcga atgtggcgcg cctgtgctcg
     1021 aactcgccgc ccttgacggc gcctagttgc tgcgcttcgt gctggcggta ctggacgagc
     1081 ggcagctcga cgaaggcgag gtccgagacc gccgcggcca acagcgcgat ccagccgtcg
     1141 tggatcaggc ggccctcggt cggtatcggc agaacgaggt cgcgcagctc ggcgcggaag
     1201 gccatcgtcg cgcccgtcac gacgttgtgt tcgagcagca cgtcgaagcc gcggccccga
     1261 cggatgagcg ccttctcctc ctcgctgaag ttgatgcgct gccacagcgt gaagccgagc
     1321 ggcgcgagcc gttcatcgac cacctcggcg tcggtgaaga cgaggcccgc gcgggggctg
     1381 gcggcgagcg cgccttccga cagctccagc ttccgcggga gccacacgtc gtcctgatcc
     1441 gcgagcgcga tgaggtcgcc cgtgcagagc gagacggcgc ggtcgaaatt tttggtcgag
     1501 ccgaggttct ccgtgttgac gtggaggcgg acggggaagc gcgcggcggc ggcgaacgcg
     1561 gcgacgaggt cggtggtgcc gtcggtcgaa cggtcgtcgc agacgaccag ctcgtcgggc
     1621 gggcgggttt gcgccgcgat gctgtcgagc tgctcccgca ggaagcgggc gccgttgtac
     1681 gtgcacatcg cgacggaaaa tttcacccga gaaaaaactc ctgccccctt gactctcttt
     1741 tgccgggcgg cgaactgtca gtcgccgccc gcggccagct cttcggcccg cgcggcgggg
     1801 cgcgcgagcg cgcgtaagtc ctcgcgcacc atctcgctca ccagcgcggc gaaagtgtac
     1861 tgcggctccc agcccagaac ggcgcgcgcc ttcgccgagt cgccgaccag cagatcgacc
     1921 tccgccgggc ggtagtagcg ctcgtccacg cggacgtact gctcccagtc gaggccgacc
     1981 tccgcgaagg cgcgctcgca gaactcgcgc accgtgtgcg tctcgccggt cgccaccacg
     2041 aagtcgtccg gctcctcctg ttgcagcatc aggtgcatcg cgcgcacgta gtctttggcg
     2101 tggccccagt cgcgcttcgc gtcgaggttg ccgagccgca gctcggtcgc cagccccgcc
     2161 ttgatgcgcg ccacggtgga cgtgatcttg cgcgtcacga actcgaagcc gcggcgcggc
     2221 ccctcgtggt tgaagaggat gcccgaggcg cagaacatgc cgtacgcctc gcggtagttt
     2281 ctggtcaggt cgaagcccgc gaccttgctg atcccgtaag ggctgcgcgg gtggaagggc
     2341 gtcgtttccc tctgcggcat ctcgcgcacc ttgccgaaca tctccgaaga ccccgcgaag
     2401 tagaagcggc aggcggggcg cagctcgcgg atcgcggcca gcacgtagtg cgtgccgttg
     2461 atgttggtgt tcatcgtcga gaagccgtcg gcgaagctct cggcgacgaa gctctgcgcg
     2521 gcgaggtggt agcactcgtc gaactggtgg cggctgaaga tgtggaaaat cgaggcgtag
     2581 gattcgaggc tcgccgggtg cagttcgacg cggtcgagca ggtggccgat gcgcccgaac
     2641 ctgcgctccg ggtcttcgag cgcgacccgc cgcaccagcc cgtgcacctc gtagcccagc
     2701 ccgagcaggt gctcggccag atagctgccg tcctgcccgg tgatgcccgt gatcaaagcc
     2761 tttttcataa tcgttgcatg atcgaccgcc ggcaccgacc tcccgctgct gtcaaacccg
     2821 ccgcgacttc ccgcgtcccg cgcccgcgac ggcgtgcagc ggcgaggcgg cggagagcgc
     2881 cctgatctga ctttcggaga cgaacggggc ggccagcgcg caggccgccc aacggactcg
     2941 ccacttcagc ccccgcccgg cggcggcggc ccgcacgaaa ctgccgacgg ccttgagcgg
     3001 cctgccgccg cgcacctgct cgatgccgaa cgcgcccctt tccgccgcgt gcatctcgcg
     3061 gttcaggcgg cgcagttcgc cgtccggcag gagggcttcg agcttgcggc gcatgagcga
     3121 catggcaccg tacatccgcg cgttgtcccg gctcgaactg ccggggtgcg agcggacgag
     3181 ggccagcgtc ccctcctgct cgacgaaccg gaagcgcttg ccggctatcg cgcagcgtat
     3241 ccagtagtcc cagtcctcca cgggcgggag cctttcgtcg aacggcccga catcggcgac
     3301 gacgccgcgc ctcaacagcg gcgagttgat gaccatgatg ttattgcgaa gcagcggcac
     3361 gagcacctcg ctgccccgtc cggagacctc gggcatccac gcgccgccgg cctcggcctc
     3421 cagtcgccgc gtccgccggc tggggtcgtc cgcgtcgaaa tagagcatat cgccgtagac
     3481 gagatcaacc tccgggtgac gctcaaggta ctcgacctgg cgctcgaact tgcgcggctc
     3541 cagcaggtcg tcggcgtcga ggaactgcac gtagacgccg cgcgcgtcgg ccagcccctt
     3601 gttcttggcg acggcctgcc gccggttccg ctggcgcagg tagcggacgc gggggtcggc
     3661 ggcggccacg cgcgccacga tctcttccgt gtcgtccgtc gagccgtcat cgacgacgac
     3721 gcactcccag tcggggtaag tctgcgcgcg caggctgtcg agcgtctcgc cgatgaaccg
     3781 cccgtagttg taggtcggca cgacgacgct gacgagcggc tgccccgttc cgtaaggcat
     3841 gtcggtggtc acttccagct ccgggcgcga tgaaaacctg cggctgtgcg gcatctcggc
     3901 gccgagtcaa tgatagaggc cgcgaaagag tcggtcgatg gggcaaggcc cgcgccgcat
     3961 ggcgcaggcg ccggggcgcc gtccggggtg aagtcgaagc cgaagatggc ggccaacgcg
     4021 tcgcgaatca tcagtggaca cctgctatcc ctgtagcccg gctccccgca cgggcgatac
     4081 gtttgagcac gtcgcgcgcc acccggtaag tccgcgtccg ggcgacggca ttgtatatct
     4141 gccgcgaata catccaaact cgcgcgtacc gcaggtcgtc cctgaagtag acgcggtagg
     4201 gcgtgcgcag ccgccgcgcc agccgcatca tcgcgaatgc ccgccgcaac ggatggagcg
     4261 cccacgaacg ttccgtcaac gccaggtatg cctcgaagga gatggcgcgc gtccccgtgt
     4321 tcctgcaaaa aacgtcgtag caggcgcgga gctgcgcctc gaaccgcgag ccggtgatcg
     4381 actcctcgtg tacgcggtag agcagcaggt gttcgggcag gttgcacagc ctgcgctcgg
     4441 ccctgattct gaaccacagg tcgtagtcct gcgcgtagcg gaaggccggg tcgtagcgcg
     4501 ggcctgccga gcggtcgaag cggaacatcg ccgccgggtg gatgagtgac ctgcgccagt
     4561 agaagttgtc aagctgctcg cctacgggca gctcccgctt gccgatcacg ttgcccgcgc
     4621 tgtcgatgtc gaaggcccac gtgctgcaag cgcccacgtc ggggttggcc tccatgaacg
     4681 ccacctgctt cgccagccgc tccggcagag aaaggtcgtc gcagtccatc cgggcgacgt
     4741 actcgccgcg ggccaggtca agccccctgt tcaggctgag cgtcaggccg agattacgct
     4801 cgttgcggac aagccttatt ctggggtcgg cgtagccgcc ggcgatctcg aagctgcgat
     4861 ccgtcgaggc gtcgtcgatg atgatgaact cgaagtcggc gaaggtctga ccgaggatgc
     4921 tgtcgatggc ctcgcgcaga tacctctcgc cgttgtacac ggccatcagc accgagactt
     4981 tcggggtcat agactcgctt ccccgccctg ttcgagccac agcccgagca cgagtaaggc
     5041 ccaggtctgg taattatcgc gcgcggcgaa ctcccgcgag gccgcgaacg gaacgaagga
     5101 gaagagcgcg tgccgcgggt cgtccagaat ccggaccttc agggcggcca cgtcggggcg
     5161 cgcgagccaa cggttgacgg gagcgccgaa gccgcacttc tcgcgcgcgc ggacagactc
     5221 cggccacgac gccccgtagc tgcggcgcag tatccacttc tcctcgcggg gcgtgatctt
     5281 cagccggctc ggcagcgaga tgcagaacga ggcgaactcc acgtcgagga agggcgcccg
     5341 cagctccagc ccgtgcgcca tcgaggcgcg gtcgatcttg accagtatgt cgccgggcat
     5401 gtagtcgcac aaatccatcc gcatggcgtc gtccacggtg cccgtcccgc cgccttcggc
     5461 ggcagccgct tcgtgcccgc cgtcgaggcc gagcagcgcc agctcctcgt cgccgaacac
     5521 ctccttctgc ttcgcgtgcg cacgggcgac cgacgcgtag ctcctccgca gccctgcgcc
     5581 gcgcgtgatc cgcccccacg cctccggggc gcgcagcccg agcgcggacg agagccgcag
     5641 tgcgcgccag aggagggcga cggcgacccg cgggtgaccg ccgcgctcca tctccca
//
LOCUS       DBNGOH010000340         3570 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2617575, whole genome shotgun sequence.
ACCESSION   DBNGOH010000340 DBNGOH010000000
VERSION     DBNGOH010000340.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3570)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3570)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3570
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2617575"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(99..1367)
                     /locus_tag="AC159P_06640"
     CDS             complement(99..1367)
                     /locus_tag="AC159P_06640"
                     /inference="COORDINATES: protein motif:HMM:NF012958.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HIR3695170.1"
                     /translation="MSRTRTAVFLKKLSVKTGALAPLVLLALAALSPQALAQAAPQPL
                     YEAPTRITIKSEVLGEERVILVRTPAAYARNDHRYPVMYMTDGDAHIEHTSGTLSFLA
                     RNNRMPEMIVVGITNTDRMRDLSPTKPEQLPVNPNFRTSGGADKFLKFIETELIPHIE
                     KNYRTQPFRTLAGHSLGGLFAIHAMVARPELFNSYISVSPALHWDNFVMIDRAKEFFK
                     NRKDYNRTLFVSLGSEPGDIGDAFGLFKDVLAKQQTKGFQWEAAKYDDEDHGSVVLRS
                     HYAGLRKTYEGWQFPRDPNTGAIRGGYKEVEAHFRGLSERLGFALHAPEPLMNQIGYI
                     MMGQNNLEDALAAFKANVERYPNSANVYDSLAEYYERQGQLELARPNYEKAVTLGKQN
                     NDPNLQIYQTTFERVTGLLSKSAAPASDKK"
     gene            1731..2564
                     /gene="mazG"
                     /locus_tag="AC159P_06645"
     CDS             1731..2564
                     /gene="mazG"
                     /locus_tag="AC159P_06645"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012112050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HIR3695171.1"
                     /translation="MPTTFDDLVALMARLRSPEGCPWDREQTYATLAPMLLEEAYEAF
                     EAVEAAREGRPLELRDELGDLLFQIVFYAQVARERGEFAIADVIEAIHTKMVRRLPHV
                     FGDVSVRDNEELLRNWEAMKAEEKRAAGREASAAEASLLDGVSAKAPALMEAYQLATK
                     AARVGFDWQRLEDIFDKLHEEIEELHEAIREHAEAAPEAEGQSARVREEVGDLLFAVT
                     NIARHLGVEPEAALKLTNRKFRRRFRHIEQGLRARGREPGAATLDEMEELWQEAKRDD
                     G"
     gene            2606..2803
                     /locus_tag="AC159P_06650"
     CDS             2606..2803
                     /locus_tag="AC159P_06650"
                     /inference="COORDINATES: protein motif:HMM:NF024183.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double zinc ribbon domain-containing protein"
                     /protein_id="HIR3695172.1"
                     /translation="MESQSNQPKDTSVCHACGAKTRQAKFCPECGEPVPAPPPACTAC
                     GHQPEAKPKFCPECGAEMPTA"
     gene            complement(2951..3199)
                     /locus_tag="AC159P_06655"
     CDS             complement(2951..3199)
                     /locus_tag="AC159P_06655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695173.1"
                     /translation="MARGWESKAVESQLEEKGVRDAEYARRYEASPEVRARRERIESL
                     RLSRARTLDQLERAATPAHREMLKRTLLAIERDVVELE"
     gene            complement(3307..>3570)
                     /locus_tag="AC159P_06660"
     CDS             complement(3307..>3570)
                     /locus_tag="AC159P_06660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695174.1"
                     /translation="TQTQGNNYLRQSSQWRRTTVNGRPALATTLTGRSPITGQNETVT
                     LITTMLSGGNVFYMAAVAPTGEYRNYQRTFDDILRSLQINDRY"
BASE COUNT          573 a         1086 c         1277 g          634 t
ORIGIN      
        1 ccgagcttcg gtcggcagac aggccgcagg cccgtccgcg cggcgcttcg gtgtgatgaa
       61 gcgtcgcgcg gacgggcctc tcggagttgc cgccgggatt acttcttgtc gctcgcgggc
      121 gcggcgctct tgctgagcag gccggtcacg cgttcgaagg tggtctggta gatttggagg
      181 ttggggtcgt tgttctgctt gccgagcgtg acggccttct cgtagttggg gcgggcgagt
      241 tcgagctggc cctgccgctc gtagtattcg gcgaggctgt cgtagacgtt ggccgagttc
      301 gggtagcgct cgacgttggc cttgaaggcg gcgagcgcgt cttccaggtt gttctggccc
      361 atcatgatgt agccgatctg gttcatcagc ggctcgggcg cgtggagcgc gaagcccagg
      421 cgctcggaga ggccgcggaa gtgcgcctcg acttccttgt agccgccgcg gatggcgccc
      481 gtgttcgggt cgcgcgggaa ctgccagccc tcgtaggtct tgcgcaggcc cgcgtagtgg
      541 ctgcggagca cgaccgagcc gtgatcctcg tcgtcgtatt tggcggcctc ccactggaag
      601 cccttggtct gctgcttcgc gagcacgtcc ttgaagaggc cgaaggcgtc gccgatgtcg
      661 cccggctcgc tgccgagcga gacgaagagc gtgcggttgt agtccttgcg gttcttgaag
      721 aactccttcg cgcggtcgat catcacgaag ttgtcccagt ggagcgcggg gctgacggag
      781 atgtaggagt tgaagagttc gggccgcgcg accatcgcgt ggatggcgaa gaggccgccg
      841 agcgagtggc cggcgagcgt gcggaagggc tgcgtgcgat agttcttctc gatgtgcgga
      901 atcaactcgg tctcgatgaa cttgaggaac ttgtcggcgc cgccgctcgt gcggaagttg
      961 ggattgaccg gcagttgctc gggcttggtc ggcgacaggt cgcgcatgcg gtcggtattg
     1021 gtgatgccga cgacgatcat ctcgggcatc cggttgttgc gcgcgaggaa cgagagcgtg
     1081 ccgctcgtgt gctcgatgtg cgcgtcgccg tcggtcatgt acatgacggg gtagcggtgg
     1141 tcgttgcgcg cgtaggcggc gggcgtgcgg acgaggatca cgcgctcctc gccgagcacc
     1201 tcggacttga tggtgatgcg cgtcggggct tcgtagaggg gctgcggcgc ggcctgcgcg
     1261 agcgcctgcg gcgagagagc ggcgagcgcg agcagaacga gcggcgcgag cgcgcccgtt
     1321 ttgaccgaca gcttcttcag gaatacggcg gtgcgggttc gtgacataat ctcctccaac
     1381 tctgttcagt gagcgcgaag cgaagcggcg ttgcgcggcg gcggcggcgg ctgttcggcg
     1441 gcgtgccccc gggggcctcg cgggaagtgt tcggcgggga ttctacagca tgcgcgccca
     1501 gccgtgtgag gcgccgccgt ttatttaccg aaattttaca agggcgtccg cgcggctgga
     1561 aacaatccga aagaatggac gcgccctcgc accccgtcgt ccgaagttcg aagttcgaga
     1621 ttcgaggttc gaggcgcgaa cctcgaagcc ccggcccgcg cggccttgaa ggttgggccg
     1681 gcctcgtcta acttgggtct cggatttcga acaccgaacc agaggtaatc atgccgacca
     1741 cattcgacga cctcgtcgcg ctgatggcgc ggctgcgctc gcccgagggg tgcccgtggg
     1801 atcgcgagca gacatacgcc acgctcgcgc cgatgctgct cgaagaagcc tacgaggctt
     1861 tcgaggcggt cgaggccgcg cgcgaagggc gcccgcttga gctgcgcgac gagctgggcg
     1921 acctgctctt ccagatcgtc ttctacgcgc aggtcgcgcg cgagcgaggc gagttcgcca
     1981 tcgccgacgt gatcgaggcc atccacacga agatggtgcg ccgcctcccg cacgtcttcg
     2041 gcgacgtgtc cgtaagagac aacgaagagc tgctccgcaa ctgggaggcg atgaaggccg
     2101 aggagaagcg cgccgcgggc cgagaagcga gcgcggcgga ggcgtcgctg ctcgacggcg
     2161 tctcggccaa agcgcccgcg ctgatggagg cgtaccagct cgcgacgaag gccgcgcgcg
     2221 tcggcttcga ctggcagcgc ctcgaagaca tcttcgacaa gctccacgaa gagatcgaag
     2281 agctgcacga agccatcagg gagcacgcgg aagccgcgcc cgaagcggaa ggccagtcgg
     2341 cgcgcgtccg cgaagaggtc ggcgacctgc tcttcgccgt caccaacatc gcgcgccacc
     2401 tcggcgtcga acccgaggcc gccctcaaac tcaccaaccg caaattccgc cgccgcttcc
     2461 gccacatcga acagggcctc agagcgcgcg ggcgcgaacc cggcgcggcg acgctggacg
     2521 agatggaaga gctgtggcag gaggccaagc gggacgacgg ctgacggcgg ggcaaaggcc
     2581 cgccgccgag atgaggcgac cgagtatgga gagtcaatcg aaccagccca aagacacgtc
     2641 cgtgtgccac gcgtgcggcg cgaagacgag gcaggcgaag ttctgccccg aatgcggcga
     2701 gcctgtaccg gcgccgcccc cggcctgcac ggcgtgcggc caccagcccg aggcgaagcc
     2761 gaagttctgc cccgagtgcg gcgccgagat gcccacggca tagagtaagg ggaaaagggg
     2821 aagaggggaa aaggcgaaaa ggatgatgag aggcacgctg cttcccatca tcgacttgat
     2881 gagaggtacg ctgcctcccg tcttcctttt cgcctcttcc cccttttacc cttttcgccc
     2941 ttcctgcgtc ctattccaac tccacgacgt cccgttcgat ggcgagcagg gtgcgcttga
     3001 gcatttcgcg gtgcgcgggt gtggcggcgc gttcgagctg gtcgagggtg cgtgcgcgcg
     3061 agaggcgcaa cgactcgatg cgctcgcggc gcgcgcggac ttcgggcgac gcttcataac
     3121 ggcgcgcgta ctcggcgtcg cggacgccct tctcttcgag ctgcgactcg acagccttgc
     3181 tctcccagcc tctcgccata gatgaactcc tcggggcgaa gagggtcaaa ggggaaaagg
     3241 cgagaaggtt ttgtctttaa ccttttcgcc ttttcccctt ttcacctttt atccccgctc
     3301 cttaggttag tagcggtcgt tgatttgcag cgagcgcagg atgtcgtcga acgttcgctg
     3361 gtagttgcgg tactcgcccg tcggcgcgac cgccgccatg tagaagacgt tgccgcccga
     3421 gagcatcgtc gtgatgagcg tgacggtctc gttctggccg gtgatgggcg agcggcccgt
     3481 gagcgtggtg gcgagcgcgg ggcgaccgtt gacggtcgtc cggcgccact ggctcgactg
     3541 ccgcaggtag ttgttgccct gcgtctgcgt
//
LOCUS       DBNGOH010000341         5479 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2251081, whole genome shotgun sequence.
ACCESSION   DBNGOH010000341 DBNGOH010000000
VERSION     DBNGOH010000341.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5479)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5479)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5479
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2251081"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="AC159P_06665"
     CDS             <1..294
                     /locus_tag="AC159P_06665"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPR end-of-group domain-containing protein"
                     /protein_id="HIR3695175.1"
                     /translation="AESLYDRGVIELNRGEYVAAQEMFERAIKREPSSADAHYALAAT
                     RARLGSVELALQALERAVELKPLLRVRAQHDIDLAMLRSEPDFERIVYSSSRA"
     gene            440..1483
                     /locus_tag="AC159P_06670"
     CDS             440..1483
                     /locus_tag="AC159P_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020714573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconeogenesis factor YvcK family protein"
                     /protein_id="HIR3695176.1"
                     /translation="MTRGLNLVAIGGGTGLSTVLSGLKGLVGRPASERVWLETLSAIV
                     TVSDDGGSSGRLREELQILPPGDIRNCMVALSEDSTLLARLFRYRFRGDGNLGGHSFG
                     NLFLAALTEITGDFVEAVRISSEVLASKGRIYPSTLNDVRLVAELEDGAEVRGETRIT
                     ASRSSIKLLRLEPEQCLPLPEALAAIRTADIITVGPGSLYTSILPNLLVARVADCIGE
                     ARAVKMFICNLMTQPGETDGYTARRHLEVVKRYAPEIHFDYVVANDRPVSEEQAAAYA
                     AEGAHQIGLTDHMLEREFGGETEVVRADLLDEGEKVRHSPEKLARVIAACYEQSLGRG
                     TTAAAPHVTSVTY"
     gene            1528..3045
                     /gene="glmU"
                     /locus_tag="AC159P_06675"
     CDS             1528..3045
                     /gene="glmU"
                     /locus_tag="AC159P_06675"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HIR3695177.1"
                     /translation="MDKQTQTPQPQQSGAGPLDILILAAGLGTRMRSGTAKVLHRLGG
                     RPLIAHVCRAAAALLKEARPIYVVVGHQAEEVKAAVREELGADGAVFITQAEQRGTGD
                     AVMSASEALADANSTLLVVSGDVPLVRAETLGALVHQHRTHRGRGAACTLLSVRLEDP
                     TGYGRVVRDEEGRFLKIVEQKDATPEERQIGEINAGIYCFETRLLFPALARVQPANAQ
                     GEYYLTDAPAILRADGEDVSVFLHAEAREVSGINTRVELAEFERLLRVRTLRNLMLNS
                     GVTVIDPQHTYVSADAQIGRDTILHPDTHVEGNTVIGEGCEIRPGTRITNSRVGNGVT
                     VKDHCVIVDSDIADNCSVGPFAHLRMNARMEEGATVGNFVEVKKSRLGTGTKAMHLSY
                     LGDADIGDRTNIGAGTVTCNYDGKNKHPTVIEEEVKIGSDTMLVAPVRVGARSVTGAG
                     SVVTADVPPDTLVAGVPAKIKKKLPREAPESTAATARGPEETAASEDAEEAAPVP"
     gene            3094..4953
                     /gene="glmS"
                     /locus_tag="AC159P_06680"
     CDS             3094..4953
                     /gene="glmS"
                     /locus_tag="AC159P_06680"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 -
                     L-glutamine:D-fructose-6-phosphate transaminase
                     (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HIR3695178.1"
                     /translation="MCGIVGYVGNKQVVPVILDGLRKLEYRGYDSAGIAVVDERHVLD
                     IRRAEGKLRNLEEAIRLKPLDGTYGIGHTRWATHGRPTEENAHPHRDCTGRVVVVHNG
                     IIENYVQLKERLKTHDHVFVTETDTEVIAHLIEEYLKEDDSFEQAVRRTCRDLRGIFA
                     LSIISTDEADTIIAVRQGPPVVIGMGDGEFFVASDVPPILQHTRDVFFLGDGELAVLT
                     RDSVRVTDFDGNSVEPQQQRITWDPIMAEKGGFKHFMLKEIYEQPRAVRDTVQGRVSL
                     DTGRVYLEEMDITPQEFAGFDRVMIAACGTSWHAGLAGKYMLEQLARVPVEVDYASEF
                     RYRDPVINEKTLLVVISQSGETADTIAALREAKERGAKILAICNVQGSMITRESDGTI
                     LTHAGPEIGVASTKAFTSQMVALYLFALHLGSLRGALTEEQARDHAQQLAELPVKIEH
                     LLNDSDEMEELAKEFHRATDFLYLGRGINFPVALEGALKLKEISYIHAEGYPAGEMKH
                     GPNALIDERLPVVVVNTRAEGDEASELRYEKTHSNIVEVKAREGQVISVLTEGDRMSS
                     TVSDHVIEVPASSDLLSPILSIIPLQLLAYHIAVRRGCDVDQPRNLAKSVTVE"
     gene            5059..5367
                     /locus_tag="AC159P_06685"
     CDS             5059..5367
                     /locus_tag="AC159P_06685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695179.1"
                     /translation="MQRSTDEDEDVPGIEGVYVEGCDQENSCYGCIEDFVLERRSAEI
                     KFTEGANLLADLEGDEGQENTLTELRITFDIDDAKYRELQANLSGVIFSGCGCFKVVV
                     "
BASE COUNT          987 a         1881 c         1770 g          841 t
ORIGIN      
        1 gccgagtcgc tctacgaccg cggcgtgatc gaactcaatc gcggcgagta cgtggcggcg
       61 caggagatgt tcgagcgggc catcaagcgc gagccttctt cggcggacgc gcactacgcg
      121 ctggcggcca cgcgcgcgcg cctcggctcg gtcgagctgg cgctccaagc gctcgaacgc
      181 gcggtcgaac tcaagcccct cttgcgcgtg cgcgcgcagc acgacatcga cctcgcgatg
      241 ctgcgcagcg agccggactt cgagcgcatc gtctactctt cctcgcgcgc gtgagcgccc
      301 tttccccctt tcgctgacga ccccgcgtcc cccccattcc tttgaaaggc caacactttt
      361 gggtatgatt ccccgcacgg gcacgttctc cgcaacgccc gccgcttctt ccgcggagac
      421 cgaaggtttg caacccgata tgacccgcgg cctcaatctc gtcgccatcg gcggcgggac
      481 ggggctttcg accgtccttt cgggcctcaa gggtctggtg ggccggcccg cttccgaacg
      541 agtgtggctc gaaacgctct cggccatcgt caccgtctcg gacgacggcg gaagctctgg
      601 ccgtctgcgc gaagagctgc aaatcctccc gcccggcgac atccgcaact gcatggtggc
      661 tctgtcggaa gactcgacgc tgctcgcgcg gctcttccgc taccgcttcc gcggcgacgg
      721 gaatctgggc ggccacagct tcggcaacct cttcctcgcc gcgctgacag agatcaccgg
      781 cgacttcgtc gaggccgtcc gcatctcctc ggaggtgctg gcgagcaagg ggcgcatcta
      841 cccttcgacg ctgaacgacg tgcgcctcgt cgccgaactc gaagacggcg cggaggtgcg
      901 cggcgagacg cgcatcaccg cttcgcgctc gtcgatcaaa ctgctccggc tcgaacccga
      961 acagtgcctg ccgctgcccg aggcgctggc cgccatccgc accgccgaca tcatcacggt
     1021 cggccccggc tcgctctaca cgagcatcct gccgaacctg ctggtggcgc gcgtagccga
     1081 ctgcatcggc gaggcgcgcg ccgtcaagat gttcatctgc aacctgatga cgcagccggg
     1141 cgagaccgac ggctacacgg cgcgccggca cctcgaagtc gtcaagcgtt acgcgcccga
     1201 gattcacttc gactacgtcg tcgccaacga ccgccccgtc agtgaggagc aggccgcggc
     1261 ctacgccgcc gagggcgcgc accagatcgg cctcacggat cacatgctcg aacgcgagtt
     1321 cgggggcgag accgaggtcg tgcgcgcaga ccttttggat gagggcgaga aggtgcgcca
     1381 cagccccgag aagctggcgc gcgtcatcgc cgcctgctac gaacagtcgc tcgggcgcgg
     1441 cacgacggca gccgcgcccc acgtcacttc ggtcacctac tgaggccgcc gaccgaccga
     1501 aagccgtcgc gccgctgagc aaagggtatg gataaacaga cacagacgcc gcagccgcag
     1561 cagtcgggcg ccggcccgct cgacatcctg atcctcgccg ccggcctcgg cacgcgcatg
     1621 cgttcgggga cggcgaaggt gctgcaccgg ctcggcggtc gcccgctcat cgcacacgtc
     1681 tgccgcgcgg cggcggcgct cttgaaggag gcgcggccca tctacgtcgt cgtcggccac
     1741 caggccgaag aggtcaaggc ggcggtgcgc gaggagctgg gcgcggacgg cgcggtcttc
     1801 atcacgcagg ccgagcagcg cggcacgggc gacgccgtga tgtccgcgag cgaggcgctc
     1861 gccgacgcga actcgacctt gctcgtcgtc tccggcgacg tgccgctcgt gcgcgcggag
     1921 accctgggcg cgctcgtcca ccagcaccgc acgcaccgcg ggcgcggcgc cgcgtgcacg
     1981 ctcctgagcg tccggctcga agacccgacc ggctacgggc gcgtcgtccg cgacgaggaa
     2041 ggccgcttcc tcaagatcgt cgagcagaag gacgcgaccc ccgaagagcg gcagatcggc
     2101 gagatcaacg ccggcatcta ctgcttcgag acgcggctcc tgttcccggc gctggcgcgc
     2161 gtgcagcccg ccaacgcgca gggcgaatac tatctgacgg acgcgcccgc catcctgcgc
     2221 gccgacggcg aggacgtgag cgtcttcctg cacgcggagg cgcgcgaggt ctcgggcatc
     2281 aacacgcggg tggagctggc cgagttcgag cgcctgctgc gtgtgcgcac cttgcgcaac
     2341 ctgatgctca acagcggcgt caccgtcatc gacccgcagc acacctacgt cagcgccgac
     2401 gcgcagatcg ggcgcgacac gattctccac cccgacacgc acgtcgaagg caacaccgtc
     2461 atcggcgagg gctgcgagat acggcccggc acgcgcatca ccaactcgcg cgtcggcaac
     2521 ggcgtcaccg tcaaagacca ctgcgtcatc gtcgattccg acatcgccga caactgctcg
     2581 gtcggcccct tcgcgcacct gcggatgaac gcgcgcatgg aagagggcgc gacggtcggc
     2641 aacttcgtcg aggtcaagaa gtcgcggctc ggcaccggca ccaaggcgat gcacctctcc
     2701 tacctcggcg acgccgacat cggcgaccgc accaacatcg gcgcgggcac cgtcacctgc
     2761 aactacgacg gcaagaacaa acaccccacc gtcatcgaag aggaagtcaa gatcggctcc
     2821 gacacgatgc tcgtcgcccc ggtgcgcgtc ggcgcgcgct ccgtcaccgg cgccggctcg
     2881 gtcgtcaccg cggacgtgcc ccccgacacg ctcgtcgccg gagtccccgc gaagatcaag
     2941 aagaagctgc cgcgcgaagc gcccgaaagc accgcggcca cggcgagagg gccggaggag
     3001 acggcggcca gcgaagacgc cgaggaagcg gcgcccgtcc cctgaactct cttacccgtt
     3061 gtccgaatcc attcccagag gtgaagtaat catatgtgtg gaattgtagg ttacgtcggt
     3121 aacaagcagg tcgtgcccgt catcctcgac gggctgcgaa agttggagta tcgcggctac
     3181 gactcggcgg gcatcgccgt ggtggacgag cggcacgtgc tcgacatccg ccgcgccgaa
     3241 ggcaagctcc gcaacctgga agaggcgatc cgtctgaagc cgctcgacgg cacctacggc
     3301 atcggccaca cgcgctgggc gacgcacggc aggcccaccg aggagaacgc gcacccgcac
     3361 cgcgactgca cgggccgcgt ggtcgtcgtc cacaacggca tcatcgagaa ctacgtgcag
     3421 ctcaaggagc ggctgaaaac gcatgaccat gtcttcgtca cggagaccga caccgaggtc
     3481 atcgcgcacc tcatcgaaga atatctgaag gaggacgact ctttcgagca ggctgtgcgg
     3541 cggacgtgcc gcgacctgcg cggcatcttc gcgctctcga tcatctcgac ggacgaggcg
     3601 gacacgatca tcgccgtcag gcaggggccg ccggtcgtca tcgggatggg cgacggcgag
     3661 ttcttcgtcg cctcggacgt gccgccgatc ttgcagcaca cgcgcgacgt gttcttcctc
     3721 ggcgacggcg agctggccgt gctcacgcgc gactcggtgc gcgtcacgga cttcgacggc
     3781 aactccgtcg agccgcagca gcagcgcatc acgtgggacc cgatcatggc cgagaagggc
     3841 gggttcaagc acttcatgct caaggagatt tacgagcagc cgcgcgccgt gcgcgacacg
     3901 gtgcagggcc gcgtctcgct ggacacaggc cgtgtctacc tcgaagagat ggatatcacg
     3961 ccgcaggagt tcgccgggtt cgaccgcgtg atgatcgccg cgtgcggcac gagctggcac
     4021 gcgggcctcg cgggcaagta catgctcgaa cagctcgcgc gcgtgcccgt cgaggtggac
     4081 tacgcctcgg agttccgcta ccgcgacccg gtgattaacg agaagacgct gctcgtcgtc
     4141 atctcgcagt cgggcgagac cgccgacacc atcgccgcgc tgcgcgaggc caaagagcgc
     4201 ggcgcgaaga ttctcgccat ctgcaacgtg cagggttcga tgatcacgcg cgagtccgac
     4261 ggcacgatcc tgacgcacgc cgggccggag atcggcgtcg cctcgacgaa ggcgttcacc
     4321 tcgcagatgg tcgcgctcta cctcttcgcg ctccacctcg ggagcctgcg cggcgcgctc
     4381 acggaggagc aggcgcgcga ccacgcgcag cagctcgccg agctgcccgt caagatcgag
     4441 cacctgctga acgactcgga cgagatggaa gagctggcga aggagttcca ccgcgcgacc
     4501 gacttcctct acctcgggcg cggcatcaac ttccccgtcg cgctcgaagg cgcgctcaag
     4561 ctcaaggaga tttcctacat ccacgccgag ggctacccgg cgggcgagat gaagcacggg
     4621 ccgaacgcgc tgatcgacga gcggctgccg gtggtcgtcg tcaacacgcg cgccgagggc
     4681 gacgaggctt ccgagctgcg ctacgagaag acgcactcga acatcgtcga ggtcaaggcg
     4741 cgcgaggggc aggtcatctc cgtcctcacc gaaggcgacc ggatgtcctc cacggtctcc
     4801 gaccacgtca tcgaagtccc cgcctcgtcc gacctgctct cgcccatcct ctcgatcatc
     4861 ccgctccaac tactcgccta ccacatcgcg gtgcgccgcg gctgcgacgt ggatcagccc
     4921 cgcaacctcg ccaagtccgt cacggtggag tagtgggaag cagtaggcgg taggcagtca
     4981 aagcaggagg ctagagagtg aatctctcaa gcctcctgct ttcttgctcg aagagcgaag
     5041 cggcttggga ctttactctt gcagcgttcc acagacgagg atgaagatgt tccgggtatc
     5101 gaaggcgtgt acgtcgaagg gtgcgatcag gagaattcct gctacgggtg catagaggat
     5161 tttgtgcttg agcgtcggag cgcagagata aaatttacag aaggtgcaaa tcttcttgca
     5221 gacttggaag gagatgaggg gcaagaaaac accctgaccg agttgcgcat caccttcgat
     5281 attgatgacg ctaagtatcg ggagttacag gctaatctct ccggtgtcat cttctcgggg
     5341 tgtggttgtt tcaaggtagt ggtatgagca tacgtctaac cggacgcgca cccagcgggc
     5401 ctctcatctt cgagggtctg tgcgcggcgg ttgtccccgg cgttagatgc ttcgtgccta
     5461 tgaaggatgc gagtaattc
//
LOCUS       DBNGOH010000342         3614 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4634226, whole genome shotgun sequence.
ACCESSION   DBNGOH010000342 DBNGOH010000000
VERSION     DBNGOH010000342.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3614)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3614)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3614
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4634226"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..233
                     /locus_tag="AC159P_06690"
     CDS             <1..233
                     /locus_tag="AC159P_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EF-Tu C-terminal domain-related protein"
                     /protein_id="HIR3695180.1"
                     /translation="RHTPFFTGYRPQFYFRTTDVTGVAHLPEGVEMVMPGDNVAMEVE
                     LITPIAMEKGLRFAIREGGRTVGAGTISDIVE"
     gene            332..655
                     /gene="rpsJ"
                     /locus_tag="AC159P_06695"
     CDS             332..655
                     /gene="rpsJ"
                     /locus_tag="AC159P_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HIR3695181.1"
                     /translation="MLNEKIRIRLKAYDHRVLDQSTSEIVETAKRTGARVAGPIPLPT
                     SKNKWTVLRSPHVDKKSREQFEIRTHKRLMDILDPTPQTVDALMKLDLPAGVDVEIKA
                     FGRDR"
     gene            775..1425
                     /gene="rplC"
                     /locus_tag="AC159P_06700"
     CDS             775..1425
                     /gene="rplC"
                     /locus_tag="AC159P_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020073852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HIR3695182.1"
                     /translation="MVNGIIGKKVGMTQLFAPDGTVTPVTVIKAGPCVVVQKKTAGGP
                     DGYDAVQLGLVDEDRPVKLKNVTKPMRGHFEKTGGGTPPTRVLKELRLDGTSELNVGD
                     KVLVDLFAEGDKIDVVGRSKGRGFAGTIKRHNFHRGPESHGSMNVRAPGSIGASAYPS
                     RVIKGTRSSGHMGDERVTIKGLTVARVDVENNLLMIRGAVPGANGCVVVVKKSTKK"
     gene            1523..2224
                     /gene="rplD"
                     /locus_tag="AC159P_06705"
     CDS             1523..2224
                     /gene="rplD"
                     /locus_tag="AC159P_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008881943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HIR3695183.1"
                     /translation="MPTVKVRNLKNEEIGEAELSEAVFGAELNESLIHSAVKAFLANA
                     RQGTVGTKTRGDVSGSGKKLWKQKGTGRARIASIRSPLWKGGGNVHGPQSRDWSQNLP
                     KKMRRGALRSALSERLREGNIVVIDEFVLGAAKTKEFVSALAALGLEGKTLVVDTVDN
                     ENLLLASRNVKRAKVVGASGVNIYDVLYHEKLVFSRSAAESLSNLLDPKSKSDGKGEE
                     EAEPQGDAQAEEEAA"
     gene            2227..2532
                     /locus_tag="AC159P_06710"
     CDS             2227..2532
                     /locus_tag="AC159P_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014263809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HIR3695184.1"
                     /translation="MRTVWDIIKAPVITEKALVAKEESADGRQLLTFRVDRRATKPEI
                     KSAVEKIFNVQVEAVRTLNYEGKLARRGRFEGRKPSWKKAYVTLKAGQEPFDYGENI"
     gene            2664..3494
                     /gene="rplB"
                     /locus_tag="AC159P_06715"
     CDS             2664..3494
                     /gene="rplB"
                     /locus_tag="AC159P_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014263810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HIR3695185.1"
                     /translation="MPIKKLTPTSPARRYQTYLTRDDLTPGAEPEKSLLEKKTRISGR
                     NNDGRITVRRRGGGHKRHYRIIDFKRDKPGVPGRVTQIEYDPNRSAHIALITYLDGEK
                     RYILSPVGLEVGKTVVSGPEADILPGNALPIRNIPLGTQIHNIELRPGKGGQMARSAG
                     SFAQLVAKEGDYAQLRMPSGEVRRVPVVCMATVGQVGNVEHENISLGKAGRSRWKGIR
                     PHVRGVAMNPVDHPHGGGEGKTSGGRHPVTPWGQPTRGYKTRKNKRTKNMIVRDRRVK
                     "
BASE COUNT          745 a         1068 c         1250 g          551 t
ORIGIN      
        1 ggcggcacac gccgtttttt acggggtacc ggccgcagtt ctactttcgg acgacggacg
       61 tgacgggggt ggcgcacctg ccggaagggg tggagatggt gatgcctggg gacaacgtgg
      121 cgatggaggt ggagttgatc acgccgatcg cgatggagaa ggggttgcgg ttcgcgatcc
      181 gagagggtgg gcggacggtg ggggcgggca ccatctcgga catcgtcgag taggtaaagc
      241 atcgccccgc tggccgccgc cgtgctcccc acgaacggcg gccaacggtg cggtcacgaa
      301 gtaaagctga cggctgagag ttatcaaaaa catgctgaac gagaagatca gaatccgact
      361 caaggcgtac gaccaccgcg tgctcgacca gtccacgtcg gagatcgtcg agacggcgaa
      421 gcgcacgggc gcgcgcgtgg cggggccgat cccgctgccc acgtcgaaga acaagtggac
      481 ggttctaagg tcgccgcacg tcgataagaa gtcgcgcgag cagttcgaga ttcgcacgca
      541 caagcggctg atggacatcc tcgacccgac gccgcagacc gtggacgcgc tgatgaagct
      601 cgacctgccc gcgggcgtcg atgtcgagat caaggcgttc ggcagggata gataagctat
      661 tagctgttag cgattggctt ttagctttta cccgattgca gggggcatgt ttgtggcccg
      721 gcagtcagtt gacggaattg gttttgctaa aagctaacag ctctgaggtt cgtgatggtt
      781 aacggaatca tcggtaagaa ggtcgggatg acgcagttgt tcgcgccgga cgggacggtc
      841 acgccggtga cggtcatcaa ggcggggccg tgcgtcgtgg tgcagaagaa gaccgcgggc
      901 gggccggacg gctacgacgc ggtgcagctc gggctggtgg acgaagaccg ccccgtcaag
      961 ctgaagaacg tgaccaagcc gatgcgcggc cacttcgaga agaccggcgg gggcacgccc
     1021 ccgacgcggg tgctcaagga gctgcgtctg gacggcacgt ccgaattgaa cgtcggcgac
     1081 aaggtcttgg tcgatctgtt cgccgagggc gacaagatcg acgtggtggg gcgctcgaag
     1141 gggcgcggct tcgcgggcac gatcaagcgt cacaacttcc accgcggccc cgagtcgcac
     1201 ggctcgatga acgtgcgcgc gcccggctcg atcggcgcgt cggcctaccc gtcgcgcgtc
     1261 atcaagggca cgcgctcctc gggtcacatg ggcgacgagc gcgtgacgat caaaggtctg
     1321 acggtggcgc gcgtggacgt ggagaacaac ctgctgatga tccgcggcgc ggtgcccggc
     1381 gcgaacgggt gcgtcgtcgt cgtcaagaag tctacgaaga agtgatgagg actgaggact
     1441 gaggactgag tgaaaaccga aaagctttta ctcagtcctc agcactcagc actcagtact
     1501 tcgtttacgg agtaaacgag caatgcctac cgtgaaggtt cgcaatctga agaacgagga
     1561 gatcggcgag gccgagctgt cggaggcggt gttcggcgcg gagctgaacg agtcgctgat
     1621 ccacagcgcc gtcaaggcgt tcctcgccaa cgcccggcag ggcaccgtgg ggacgaagac
     1681 gcgcggcgac gtgtccggct ccggcaagaa gctctggaag cagaagggga cgggccgggc
     1741 gcgcatcgcc tcgatccgct cgccgctgtg gaagggcggc ggcaacgtgc acgggccgca
     1801 gtcgcgcgac tggtcgcaga acctgccgaa gaagatgcgg cgcggcgcgc tgcgctcggc
     1861 gctgtcggag cggctgcgcg agggcaacat cgtcgtcatc gacgagttcg tgctcggcgc
     1921 ggccaagacg aaggagttcg tctcggcgct ggcggcgctc ggcctcgaag gcaagacgct
     1981 ggtcgtggac accgtggaca acgagaacct gctcctggct tcgcgcaacg tgaagcgcgc
     2041 gaaggtggtc ggcgcgagcg gcgtcaacat ctacgacgtg ctctaccacg agaagctcgt
     2101 cttctcgcgc tccgccgccg agagtctgtc gaacctgctc gacccgaagt cgaagtcgga
     2161 cggcaagggc gaggaagagg ccgagccgca aggcgacgcg caggctgaag aggaggcggc
     2221 gtagagatga ggactgtgtg ggacatcatc aaggcgccgg tgattaccga gaaggcgctc
     2281 gtggcgaagg aggagtcggc ggacggtcgc cagctcctga ccttccgggt tgaccgccgc
     2341 gcgaccaagc cggagatcaa gtcggcggtc gagaaaatct tcaacgtcca ggtggaagcg
     2401 gtgcgcactc tcaactacga gggcaagctc gcgcgccgcg gtcgcttcga gggccgcaag
     2461 ccttcgtgga agaaagccta cgtcacgctc aaggcggggc aggagccgtt cgactacggc
     2521 gagaacattt aagagcggtt agctgtcagc tcttagcttt tagctagttc ctgacagcgc
     2581 tcggccaaag ttcaaatcga agcggtcagc tcgcggaagg tttttgctaa cagctaaccg
     2641 ctaatagcta acagctaaaa gctatgccca tcaagaaact gacaccgaca tcgccggcgc
     2701 ggcgctacca gacgtacctg acgcgcgacg atctgactcc gggcgcggag cctgagaagt
     2761 ctctgctgga gaagaagacg cgtatctccg gtcggaacaa cgacgggcgc atcacggtgc
     2821 ggcgtcgcgg cggcggccac aagcgccact accgcatcat cgacttcaag cgcgacaagc
     2881 caggcgtgcc gggccgcgtc acgcagatcg agtacgaccc gaaccgctcg gcgcacatcg
     2941 cgctcatcac ctacctcgac ggcgagaagc gctacatcct ctcgcccgtc gggctggagg
     3001 tcggcaagac cgtggtcagc ggccccgagg cggacatcct gcccggcaac gctctgccca
     3061 tccgcaacat cccgctcggc acgcagattc acaacatcga gctgcgcccc ggcaagggcg
     3121 ggcagatggc gcgctccgcc ggctcgttcg cgcagctcgt ggcgaaggaa ggcgactacg
     3181 cgcagctccg catgccttcg ggcgaggtgc ggcgcgtccc cgtggtctgc atggcgaccg
     3241 tcgggcaggt cggcaacgtc gagcacgaga acatctcgct gggcaaggcc gggcgctcgc
     3301 gttggaaagg catccgcccg cacgtccgcg gcgtcgccat gaacccggtc gatcacccgc
     3361 acggcggcgg cgagggcaag acctcgggcg gtcgccaccc cgtcaccccg tggggccagc
     3421 cgacccgcgg ctacaagacg cgcaagaaca agcgcacgaa gaacatgatc gtgcgcgacc
     3481 ggagagttaa atagatttta gatgtcagat gctggatgtt agtaaaaact cagcactgac
     3541 atctgacatc cagcatctga catccagttg agcgaagcga gaaaaccatg gcacgttcag
     3601 tcaaaaaagg gccg
//
LOCUS       DBNGOH010000343         2416 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3167954, whole genome shotgun sequence.
ACCESSION   DBNGOH010000343 DBNGOH010000000
VERSION     DBNGOH010000343.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2416)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2416)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2416
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3167954"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1011
                     /locus_tag="AC159P_06720"
     CDS             <1..1011
                     /locus_tag="AC159P_06720"
                     /inference="COORDINATES: protein motif:HMM:NF015506.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HIR3695186.1"
                     /translation="IRRGFDDITRLLRDPASADTSPTPSDAVVEKVRREAAAEGRREE
                     TGDAGRSAEAEARKPADDAAVRRAGEERREAPHAREKAEEAARIEAREELQRRVEESR
                     RRVAEAARQKSEEAARRHAEDETRGEVAGPASTPEHESQEPPTGSGVKRCPKCGTTYQ
                     SDILAYCVYDSALLISDDEPSYRGAVGSHVSATGSPYAIRPRVLGLVALTLLGGSLIG
                     YLINRSISTGPGATGTAQQEAARLQRERPATDGALKGRETSLPDPDYPTAARREGVSG
                     KVTVAVTVNNKGEVIAARALGGHPLLQAAAERAARAAKFSTEKQARGATRTSGTITYD
                     FK"
     gene            complement(1089..1247)
                     /locus_tag="AC159P_06725"
     CDS             complement(1089..1247)
                     /locus_tag="AC159P_06725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695187.1"
                     /translation="MFGGARGDSTFKSGKTTYRAYAAYRAAVGFGGLIPSDPDNVRLL
                     TDTQFAGQ"
     gene            1636..2004
                     /locus_tag="AC159P_06730"
     CDS             1636..2004
                     /locus_tag="AC159P_06730"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR3695188.1"
                     /translation="MKPHAGRVLCVEDDPDTCTMLCALFALAGCEVETAATFAEGVEK
                     AAAGRFDLFVLDNWLPGGSGTELCRELRRRWPSTPVLFYSAAGYDTDREEALAAGAAD
                     YLVKPADVGRLVETVKGLLG"
BASE COUNT          458 a          750 c          841 g          367 t
ORIGIN      
        1 atcaggagag gtttcgatga cataactcgc ctgcttcgcg accccgcgtc tgccgataca
       61 tcccccacac cgagcgacgc cgtagtcgag aaagttcgaa gggaggcggc ggcagaaggg
      121 cgtagagaag agacgggcga cgcgggcagg agcgccgagg cggaagcccg aaaaccggca
      181 gacgatgcgg cggtaagaag ggcgggagag gagcgccggg aggcacctca cgcgcgggaa
      241 aaggccgaag aggcggcacg aatagaagcc agggaggaat tacagcggag ggtagaggag
      301 tctcgcaggc gtgtcgccga agcagcccgt cagaagtcgg aggaggctgc gcgccgccat
      361 gccgaagacg agacgcgggg cgaggtggcc ggccccgcat cgacgcccga acacgaatct
      421 caagagccgc ccacgggttc gggcgtcaaa cgctgcccga agtgcggcac cacctatcag
      481 agcgacatcc tggcgtactg cgtgtatgac tccgcccttt tgataagcga tgatgagcct
      541 tcttacaggg gcgccgtcgg atcacacgta tcggcgacgg ggagtcctta cgccattagg
      601 ccgcgggtgt tgggattggt cgccctcacg ctcttgggag gcagtctgat cggctacctc
      661 atcaacagga gtatctctac cggccccggc gcgaccggca cggcgcaaca ggaggccgcc
      721 cgcctacagc gggaacgacc cgccaccgac ggcgcgctca aaggcaggga gaccagttta
      781 cccgacccgg attatccgac ggcggctaga cgcgaagggg tctcgggcaa ggtcacggtg
      841 gcggtcacgg tgaacaataa aggtgaggtt attgcggcgc gtgcgctggg tggccacccg
      901 ctactccaag cggctgccga aagggcggcg cgggcggcga aattctcgac cgaaaaacag
      961 gcccgcggag cgaccaggac gtcaggcaca atcacgtacg acttcaagta agggtacatc
     1021 ccgaacacgc agcgccggcg tactgccggg gtgaggccgg taaggcggac tgcctcgccg
     1081 ccgggaattc attgcccggc gaactgcgtg tccgtcagca gtcgcacgtt gtcggggtcc
     1141 gaggggatga ggccgccgaa gccgaccgcc gcccgatagg ccgcgtaagc cctgtaagtc
     1201 gtcttgccgc tcttgaacgt ggagtcgccg cgcgcgccgc cgaacacggc ccagtccgtg
     1261 cgccgacggc gggacgagga gggagaacag gaggaagaac aggacgagag gccgggacgg
     1321 tctgcgggtt gactgggtca tgcgactcct ttcgagcggg gcctgcactt cgatgtcatg
     1381 acatggcggc gcggggacaa agaagcggaa ggccgacggg ccaccgcgct ggcggacctc
     1441 cgtgtgcggc gattatttct acgcgcacgg tggccgcatg tcaacgaccg tcgagcggga
     1501 cgccgaggct gctcggagag cttgagacgc gtgcgtgaag ccaagccgtt gacacccgcg
     1561 cccccgccgg aatatcttcc cacggctcga tcccattccc ggcgcacccc gatgcgcgcc
     1621 cgtacggaga cacccttgaa gcctcacgcc ggacgtgtcc tctgcgttga agacgacccc
     1681 gacacctgca cgatgctctg cgccctgttc gccctcgccg gctgcgaggt cgagacggcg
     1741 gccaccttcg cggagggtgt cgagaaggcc gccgccgggc gcttcgacct cttcgttctg
     1801 gacaactggc tgccgggcgg ctcgggcacg gagctgtgcc gcgagctgcg ccgccgttgg
     1861 ccctccacgc ccgtgctctt ctactcggcg gcgggttacg acactgaccg ggaggaggcg
     1921 ctcgcggcgg gtgccgccga ctacctggtc aagcctgccg acgtggggcg tctggtggag
     1981 accgtcaaag ggctgctggg gtgagacccc gctccggacg acgtgcgctg cgcgcgcgtg
     2041 cgctctttcg gcaccgcatc gatagggtga aagagtcgat tcggccacgg cggctgcatg
     2101 agatcgcgtc tgcgtaatac ctccggcggc gccttgctgc gtaagatgaa cggggttcta
     2161 tcaaaagagt caggtagggc cggcttgcgg ttttggttct gataacgacc gattaaaggt
     2221 ggttctcccg taattttgat actgctggcg aattcaccgc gacccgatga ttgccgcgaa
     2281 atcccgagcc cgcagggtgg cgggcggttt caattcggcg ccgttgcgta ttccggtcga
     2341 aagtgaccac ccgttccact gatagctggc cgcccattcc gccaaggtga ccacccattc
     2401 cggaaagtgg tacacc
//
LOCUS       DBNGOH010000344         3996 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4451886, whole genome shotgun sequence.
ACCESSION   DBNGOH010000344 DBNGOH010000000
VERSION     DBNGOH010000344.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3996)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3996)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3996
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4451886"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1001
                     /locus_tag="AC159P_06735"
     CDS             <1..1001
                     /locus_tag="AC159P_06735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695189.1"
                     /translation="GLGTDFDVLTLFGALAAASGFEVRFACTSDRQDNFFDPQFADDY
                     FIRPTSIAVRVGEAWRFFDPASTYVPFGMLRWQEEGQPTLIADAKETIWVISPISAPE
                     RSLERRTAKLKLSEDGTLEGDVRVEYTGHFAADMKEYHDDESADSREEILKNRFQSRM
                     GGVEVTSVKVENVTDPVKPFAYTLHIKVPAYAARTGKRLLFQPAFFQRGVGALFPTSV
                     RRHHVYFHYPWAEEDKVEIELPAGFALDNAEAPTPISAGGLTKYQPRLMITKDGRTLT
                     YERKFHFGDATAGGNSLIFPTNAYAQLKTFFDELHKQDGHTLSIKQAAAAAPTASN"
     gene            1121..3088
                     /locus_tag="AC159P_06740"
     CDS             1121..3088
                     /locus_tag="AC159P_06740"
                     /inference="COORDINATES: protein motif:HMM:NF024371.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 domain-containing transglutaminase
                     family protein"
                     /protein_id="HIR3695190.1"
                     /translation="MPYRLINCCLVLAALAATAVAATPAPGDDVPAWLKQAAGTTAPA
                     YDKKVEVVVLHDEGTMTVNADGRVMNVATFAIRVLTRDGRRAATAYTSYATGTDKVKE
                     MRAWLIRPDGSVKKYGKNETLDEAVSLDDVYNDYRARKISAVDDADVGCVFGYQITTE
                     ERPFFPQAKWYFQGYYPVLYSRMTVVLPAGWSAQGVTFNHPGVEPKVNGTSYSWELRN
                     LAPIEDEPASPSVQTLAPRLAINYAPPASAGFRSFSTWADVSRWYTELADPQSEPDEA
                     IAAKTRELTAGAKTELEKIQAIGRFVQGIQYISIQIGIGGYRPHKATEVFAKKYGDCK
                     DKATLMRAMLKSIGMQSYHVLIFSGDRTYVRQEWASPSQFNHCIIAVRVGAGTAAPSV
                     VEHAQLGRLLIFDATDEDTPVGDLPDHEQGSFALVAAGDTGALLRMPITPPETNHLER
                     TAEVTLEATGAIRATVRDRAAGQSAVQWRRLFKARSRPDFGKAVERWITGGTATGAVL
                     SKVEPTDDHTGGRFSLDMEFAADRYAQSMMDRLLVFKPALVRRGDSVRLAEPTRKHPV
                     VLESEVFSETVRFKLPDGFAVDEMPDAVKLNSDFATYTASYEVKDGHLLFTRKLIQLA
                     TTIPAEQYKSVRDFFGRIRASEEAPIVLAKK"
     gene            3205..3747
                     /locus_tag="AC159P_06745"
     CDS             3205..3747
                     /locus_tag="AC159P_06745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695191.1"
                     /translation="MNSRRRNLTALALLVLLAAAAFGQDAGDRNVRRLPVPGRTWAVE
                     IALPNFNVRHDALSADGRARKLRADIESEGYVVTVTIVPASTPRVSSKDLRDLAAERV
                     RAGVIVRRENIKTSDYKKTPTLEYLVRDFRGQSLYQKHLNAYVSRDNVWIDIHFSKAP
                     FRDGDEKLFYAILDTLKFTQ"
BASE COUNT          703 a         1487 c         1236 g          570 t
ORIGIN      
        1 gcggcctcgg caccgacttc gacgtgctca cgctcttcgg cgcgctggcc gcggcctccg
       61 gcttcgaggt gcgcttcgcc tgcacctccg accgccagga caacttcttc gacccgcagt
      121 tcgccgacga ctacttcatc aggccgacca gcatcgccgt ccgcgtcggc gaagcctggc
      181 gcttcttcga cccggcgagc acctacgtcc ccttcggcat gctgcgctgg caggaggagg
      241 ggcagcccac gctcatcgcc gacgcgaagg agacgatctg ggtcatcagc cccatctcgg
      301 cgcccgaaag atcgctggag cggcgcaccg ccaagctcaa gctctccgag gacggcacgc
      361 tcgaaggcga cgtgcgcgtc gagtacacgg gccacttcgc cgccgacatg aaggagtatc
      421 acgacgacga gtccgccgac tcgcgcgagg agattctgaa gaaccgcttc cagtcgcgga
      481 tgggcggcgt cgaagtcacc agcgtcaagg tcgagaacgt gaccgacccg gtcaagccct
      541 tcgcctacac gctccacatc aaggtgcccg cttacgcggc gcgcaccggc aagcgcctgc
      601 tcttccagcc cgccttcttc cagcgcggcg tcggcgcgct cttcccgacc agcgtgcgcc
      661 gccaccatgt ctacttccac tacccgtggg ccgaggaaga caaggtcgag atcgagctgc
      721 ccgcgggctt cgcgctcgac aacgccgagg cgcccacgcc gatctccgcg ggcggcctca
      781 cgaagtacca gccccgtctc atgattacga aggacgggcg cacgctcacc tacgagcgca
      841 agttccactt cggcgacgcg accgccggcg gcaactcgct gatcttcccg acgaacgcct
      901 acgcgcagct caagaccttc ttcgacgagc tgcacaagca ggacggccac acgctctcga
      961 tcaagcaggc cgccgccgcc gcgccgacgg cctcgaactg acccgcgccg aacggacgcg
     1021 gacgcggccc gacttcccga cacgcgtgcc gccccggcgc gggactccgt gcgacaagcc
     1081 gcagtcccgc gccttcgacc caccgagagg ttcaagaccg atgccctacc gcctgatcaa
     1141 ctgctgcctc gtcctcgccg cgctcgccgc gacggcggtg gccgcgacgc ccgcccccgg
     1201 cgacgatgtc cccgcgtggc tcaagcaggc ggcgggcacc accgcccccg cctacgacaa
     1261 gaaggtcgag gtcgtcgtcc tgcacgacga gggcacgatg acggtcaacg ccgacggtcg
     1321 cgtgatgaac gtcgccacct tcgccatccg cgtcctgacg cgcgacgggc gccgcgccgc
     1381 cacggcctac acctcctacg cgaccggcac cgacaaggtc aaggagatgc gtgcgtggct
     1441 catccgcccc gacggctcgg tcaagaagta cggcaagaac gagacgctcg acgaggccgt
     1501 gagcctcgac gacgtgtaca acgactaccg cgcgcgcaag atttccgccg tggacgacgc
     1561 cgacgtgggc tgcgtcttcg gctaccagat cacgaccgag gagcgcccct tcttcccgca
     1621 ggccaagtgg tacttccagg gctactaccc ggtgctctac tcgcgcatga cggtggtgct
     1681 gcccgccggt tggagcgcgc agggcgtcac cttcaaccac cccggcgtcg agccgaaggt
     1741 caacggcacc tcctactctt gggagctgcg gaacctcgcg cccatcgagg acgagccggc
     1801 cagcccctct gtgcagacgc tcgcgccgcg gctcgccatc aactacgcgc cgccggcgtc
     1861 tgccggcttc cgctcgttct cgacctgggc cgacgtgtcg cgctggtaca cggagctggc
     1921 cgacccgcag tccgagccgg acgaggccat cgccgccaag acgcgcgagc tgaccgccgg
     1981 cgccaagacc gagcttgaga agattcaggc catcggtcgc ttcgtccagg gcatccagta
     2041 catctcgatc cagatcggca tcggcggcta ccgcccgcac aaggcgaccg aggtcttcgc
     2101 caagaagtac ggcgactgca aggacaaggc gacgctcatg cgcgcgatgc tgaagtccat
     2161 cggcatgcag tcctaccacg tgctcatctt ctcgggcgac cgcacctacg tgcggcagga
     2221 gtgggcctcg ccctcgcagt tcaaccactg catcatcgcg gtgcgcgtcg gcgcgggcac
     2281 cgcggcgccg agcgtcgtcg agcacgcgca gctcgggcgc ctcctcatct tcgacgcgac
     2341 cgacgaggac acgcccgtcg gcgacctgcc cgaccacgag caggggagct tcgcgctcgt
     2401 cgccgcgggc gacacgggcg cgctgctgcg gatgccgatc acgccgcccg agacgaacca
     2461 ccttgagcgc accgccgagg tgacgctcga agcgacgggc gccatccgcg ccaccgtccg
     2521 cgaccgcgcc gccggccagt ccgccgtcca gtggcgccgc ctcttcaagg cgcgctcgcg
     2581 gcccgacttc ggcaaggcgg tcgagcgctg gatcacgggc ggcacggcga cgggcgccgt
     2641 cctctcgaag gtcgagccga cggacgacca cacgggcggg cgcttctcgc tcgacatgga
     2701 gttcgccgcc gaccgctacg cgcagtcgat gatggatcgc ctgctcgtct tcaagccggc
     2761 gctcgtccgg cgcggtgact ccgtgcggct cgccgagccg acgcgcaagc acccggtcgt
     2821 gctcgaatcc gaggtcttct ccgagaccgt ccgcttcaag ctgcccgacg gcttcgccgt
     2881 ggacgagatg cccgacgccg tcaagctcaa ctccgacttc gccacctaca cggcaagcta
     2941 cgaggtgaag gacggccacc tgctcttcac gcgcaagctc atccagctcg ccaccaccat
     3001 ccccgccgag cagtacaagt ccgtccgcga cttcttcggt cgcatccgcg ccagcgagga
     3061 ggcgcccatc gtgctggcga agaagtaaga gccgcgcaag acccgcgaac gaggaacgcg
     3121 gccaccgctc gcacgcgggc gacggtggcc gcgttcctcg tttgcgcggc acccccgcgc
     3181 gggctaacat ccgccgacag ggacatgaat tcacgacgga gaaatctaac ggcgctcgcc
     3241 ctcctcgtcc tgctcgccgc cgccgcgttc gggcaagatg ccggcgaccg gaacgtgcgg
     3301 cggctgcccg tgccgggccg cacgtgggcg gtggagatcg cgctgccgaa cttcaacgtc
     3361 aggcacgatg ccctgagcgc cgacgggcgc gcgcgcaagc tccgcgccga catcgagtcc
     3421 gagggctacg tcgtcaccgt caccatcgtc cccgcttcga cgccgcgcgt cagcagcaaa
     3481 gacctgcgcg acctcgccgc cgaacgcgtc cgcgccgggg tgatcgtccg ccgcgaaaac
     3541 atcaagacct ccgactacaa gaagacgccg accctcgaat acctcgtccg cgatttcaga
     3601 ggccagtccc tctaccagaa gcacctcaac gcctacgtct cccgcgacaa cgtctggatc
     3661 gacatccact tctccaaagc ccccttccgc gacggcgacg aaaaactctt ctacgccatc
     3721 ctcgacacgc tcaagttcac tcaataaaag cagtctggag tctgaagaag acagataagg
     3781 agacgcttgg gactttacct ctcaagcttt ctcttcgttc tgactccaga cttcagactc
     3841 cagactccag actttttagg cggaagtccg cgacgagcgg gaggtggtcg gaggcgacga
     3901 gcgcggtgag gctgcggtgg agcgtgaggc gttcgagttc gagcgcgggg tcgtggtaga
     3961 cgtggtcgag atgaagaaag gggagcggcc ccggat
//
LOCUS       DBNGOH010000345         6561 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4452012, whole genome shotgun sequence.
ACCESSION   DBNGOH010000345 DBNGOH010000000
VERSION     DBNGOH010000345.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6561)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6561)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6561
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4452012"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(91..741)
                     /locus_tag="AC159P_06750"
     CDS             complement(91..741)
                     /locus_tag="AC159P_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007918361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3695192.1"
                     /translation="MSEDITVMLVDDHQIVRQGVRALLETQPDIQIVGEASSGEEGVR
                     LASEHAPDVVLMDLVMPGMNGVEATRHIKQESPRTQVIVLTSYHEDEHILPAIRAGAL
                     SYLLKDVSPAELLSAVRKAARGETTIHPRVAAKMMQALHGAGEKEESPFADLSKREVE
                     VLRLIAEGMPNAAIAETLFISEKTVKSHVGNILSKLHLADRTQAAVYAWRSGLVDQ"
     gene            complement(753..2051)
                     /locus_tag="AC159P_06755"
     CDS             complement(753..2051)
                     /locus_tag="AC159P_06755"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3695193.1"
                     /translation="MIVAGLAQELTPYVESSPANEAALSLWLKRQNEKIEEGVGDWHG
                     LSFYSDPSIYSAVVDTRGEILAESSAGRRPAAGQRGAATIESFIHAALDGETDPARLS
                     AVEGDGALVAAAPIRADGGRIVGALVFHTYAPFDWRVHLSKVAKGLAAPLLVLIMLAL
                     VVGIGFGLVTTRRLVKRLEVISGAAGEWARGDFSASVVDEKADAVGQLVRRLNSMAAE
                     LRGVLDLRQELAGMEERTRLARDLHDTVKQQVFALGMQIGAAQVMLDGGPPEAHRRLA
                     EAEELVRQIQHELVTIIKELQPAGRNGKGFEHALREHVANWSRQSGVTAEVELEQGLQ
                     LTPEAEHALLRITQEALSNVSRHGRATRARVGLQRGEAGGVALTIEDDGAGFDVSHRN
                     GGMGLRNMRERAEALPGGWFRVESQTGKGTSVTAGARAAN"
     gene            complement(2319..2951)
                     /locus_tag="AC159P_06760"
     CDS             complement(2319..2951)
                     /locus_tag="AC159P_06760"
                     /inference="COORDINATES: protein motif:HMM:NF033545.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HIR3695194.1"
                     /translation="MPRPCAGRSSGSTCRVKKSLAASERVEADRQAFRRRVERLDRRR
                     LVFTDETGFHLAMTRAYGRAARGERVNRRVPRNHGKGVTLLGALGLRGLLAPFSLEGA
                     LDAVAFDAYVSRMLVGQLRPKDVVLLDNLPVHQASQVEAAVAGVKAEVMWLPAYSPDF
                     SPIEECWSKVKALVRGREPRTPKELNAAPSDALASVTLDDIDGWFRHCGY"
     gene            complement(2905..3246)
                     /locus_tag="AC159P_06765"
                     /pseudo
     CDS             complement(2905..3246)
                     /locus_tag="AC159P_06765"
                     /inference="COORDINATES: protein motif:HMM:NF013842.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 transposase-related protein"
     gene            3320..3865
                     /locus_tag="AC159P_06770"
     CDS             3320..3865
                     /locus_tag="AC159P_06770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695195.1"
                     /translation="MGDDAEFYIEQQEEEARFKQASEYAALDSKRKPVLCWADGVGYE
                     DEIWSWEPMNRILGIFSNLHAERRIGSDFFLASRVPIEGDEEEWDNEYASRPESDDRR
                     EIKFVDELVFYVANSEADATHEVIVLSRSDADLLREEVVRQKSTAKTSKEKMLAEMLE
                     EIVSVIEADSQRNGFVFARTL"
     gene            3911..4291
                     /locus_tag="AC159P_06775"
     CDS             3911..4291
                     /locus_tag="AC159P_06775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695196.1"
                     /translation="MLKAAGLRLNRDLSDGEDEGEALIAEFVRVAGMAHTGIFIGDSV
                     YLREFNKKATWALMPHLVATARTNEDGRLTLRAAKPGSYYLMGGAPPPAHAVWTMPIT
                     LDRDQAVRLDSSNTMAASRANDAN"
     gene            complement(4472..5053)
                     /locus_tag="AC159P_06780"
     CDS             complement(4472..5053)
                     /locus_tag="AC159P_06780"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012527605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HIR3695197.1"
                     /translation="MRRLKIIEHISLDGVIQHSADDDDFPYSDWTASYRTPAGRDAIL
                     AAYGGSFDLLLGRRTYDIWSRFWPKAPSSPMADGLNAATKFVATHRPESLEWGPFEGL
                     GPDIVEGVRRVKSQDGPDLILSGSSTLTSTLLEHGLADEVLLVVYPVLLGTGKRCFAE
                     KTPPRSFELVSTKAFPSGIVFSAYKVAGPLKTG"
     gene            complement(5117..5785)
                     /locus_tag="AC159P_06785"
     CDS             complement(5117..5785)
                     /locus_tag="AC159P_06785"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009556382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HIR3695198.1"
                     /translation="MCGRFAQRSPAKKITKKFKVEEVPPLVERYNVAPTQTVLAVREA
                     SDGREATFLKWGLVPRWAKNPSIGNKLINARAETVTEKPSFRDAFSRRRCLVPMEGFY
                     EWARRGDRKRPFYFHMRDGEPFAVAGLWESWEGGGELLETCTLLTTEANELLAGYHDR
                     MPAILRPEDYDLWLDAGVRSAERLLPLVRPYPREEMTTYAVGLMVNSPSNDSPRCVEP
                     AREN"
     gene            5887..6327
                     /locus_tag="AC159P_06790"
     CDS             5887..6327
                     /locus_tag="AC159P_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007917640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HIR3695199.1"
                     /translation="MTYVLWIIQALLALLFLFAGGTKLVIPPDVLASMGSPNQIPLPG
                     WFVRFIGVAEVLGALGLILPGLLRIKPRLTPLAAAGLMVIMIGATALAMAADGVAAGM
                     VPLAVGLLAVFVAYGRWRLAPHPARREMIRGRSSRERQEQLKGE"
     gene            6329..>6561
                     /locus_tag="AC159P_06795"
     CDS             6329..>6561
                     /locus_tag="AC159P_06795"
                     /inference="COORDINATES: protein motif:HMM:NF024865.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YybH family protein"
                     /protein_id="HIR3695200.1"
                     /translation="MTTENSRADDEARIRELIEERVRTIRAKDVDALMSNHAPDIVMF
                     DALDPLRYVGSEAVRKRAGQWLSWYQGPVGYEVR"
BASE COUNT         1143 a         2155 c         2080 g         1183 t
ORIGIN      
        1 aaagaccggc aggtgcgttc tgattttcat ggttgactct cctgatattc cctcgagatc
       61 acctgaaccg ggtgaccgag tccggaagtc ttactggtcg acgagtccgc tgcgccaggc
      121 gtagacggcg gcctgcgtgc ggtcggcgag gtggagcttc gagaggatgt tgccgacgtg
      181 tgacttgacg gtcttctcgc tgatgaagag cgtctcggcg atggcggcgt tcggcatccc
      241 ctcggcgatg agccgcagca cctccacctc gcgcttgctc aggtccgcga aagggctctc
      301 ctccttctcg cccgcgccgt ggagcgcctg catcatcttc gccgccactc gcggatggat
      361 ggtcgtctcg ccgcgcgccg ccttgcgcac cgccgagagc agctcggccg ggctcacatc
      421 tttcagaaga tacgagagcg cgccggcgcg gatggccggc aggatgtgct cgtcctcgtg
      481 gtacgaggtc agcacgatga cctgcgtgcg tgggctctcc tgtttgatgt ggcgcgtcgc
      541 ctcgacgccg ttcattccgg gcatgacgag gtccataagc accacgtccg gcgcgtgctc
      601 cgaggcgagg cgtaccccct cctcgcccga cgacgcctcg ccgacgatct ggatgtcggg
      661 ctgggtttcg agcagcgcgc gcacgccctg ccgcacgatc tggtggtcgt caacgagcat
      721 gaccgtgatg tcttcagaca ttgcccctcc gttcaattag cggcgcgcgc cccggcggtg
      781 acgctcgtgc ccttccccgt ctgactctcg acgcggaacc acccaccggg aagcgcctcg
      841 gcgcgctcgc gcatgttcct gaggcccatc ccgccgttcc tgtggcttac gtcgaagccg
      901 gcgccgtcgt cctcgattgt gagcgccacg ccgccggcct cgccccgttg gagcccgaca
      961 cgcgcgcgcg tcgcacggcc gtgccgcgag acgttcgaga gcgcctcctg cgtgatgcgc
     1021 aggagggcgt gttcggcctc gggcgtgagc tgtagcccct gctccaactc tacctccgcg
     1081 gtgacgccgc tctgccgcga ccagttcgcg acgtgctcgc ggagcgcgtg ctcgaacccc
     1141 ttgccgttcc ggcccgccgg ctgaagctct ttgatgatcg tgaccagctc gtgctgaatc
     1201 tggcgcacca gttcctcggc ctcggcgagc cgcctgtgcg cctccggggg cccgccgtcg
     1261 agcatcacct gcgcggcccc gatctgcatg cccagcgcga agacctgctg cttgaccgtg
     1321 tcgtgcaggt cgcgcgcgag ccgggtgcgc tcttccatcc cggccagctc ctgcctgaga
     1381 tcgagcaccc cgcgcagctc cgccgccatc gagttcaatc tcctcaccag ttgccccacc
     1441 gcatccgcct tctcatccac tacggacgcc gagaagtcgc cccgcgccca ctcgccggcg
     1501 gccccgctga tcacttcgag ccgcttgacc aaccgccgcg tcgtcaccag gccgaacccg
     1561 atgccgacga ccaaggcgag catgatgagc accaagagcg gcgcggccag ccctttggct
     1621 actttggaca gatgcacgcg ccagtcgaac ggggcgtacg tgtggaagac gagtgcgccg
     1681 acgatgcgcc cgccgtcggc tctgatgggc gccgcggcga ccagcgcgcc gtcgccttcg
     1741 accgccgaca gccttgccgg gtcggtttcg ccatcgagcg cggcgtggat gaaactttcg
     1801 atagtcgccg cccccctctg tcctgcggcc ggccgccgcc ccgcggagga ttcggccaaa
     1861 atctcgcccc gcgtgtcgac caccgcggag tagatcgacg ggtcggagta gaaggatagg
     1921 ccgtgccagt cgcccacccc ctcctctatc ttctcgttct gccgcttgag ccagagggag
     1981 agcgccgcct cgttcgccgg cgacgattcg acgtaagggg tcagttcctg cgcgagcccc
     2041 gcgacgatca ccgcggcgac gtccggccgg tggagtgtac ggtatttaag gtaggagaag
     2101 attacgcccg cggccacggc cgccaggatc agccccattg tgacgcccac gtaaagaagc
     2161 gtcagccgcc accgcagctt ctggtgccaa cgaattgtgc cggcctgtcg catcccttcc
     2221 cctttccgaa gcgaattata gacccggcac tcccgcccgg ccacgggctc tggggcgatt
     2281 cgcactcaga cttatagcgc tttgcacttg cattgagact aatagccgca atgcctgaac
     2341 cagccgtcaa tatcgtcgag cgttacggac gcgagtgcgt cactcggcgc cgcgttcaac
     2401 tccttcggcg tgcgcggctc tcgcccgcgc accagcgcct tgaccttcga ccagcactcc
     2461 tctatcggcg agaagtccgg cgagtaagcc ggcagccaca tcacctccgc cttgaccccg
     2521 gcgaccgccg cctcgacttg gctggcctga tgcaccggca ggttatcgag caacaccacg
     2581 tccttcgggc gcaactgccc gaccagcatc cggctgacgt aagcatcgaa ggccaccgca
     2641 tcgagcgcgc cctccaggct gaacggggcg agcagcccgc gcaggcccaa agccccgagc
     2701 agggtgacgc ccttgccgtg gttgcgcggc acgcgccggt tgacccgctc gccgcgcgcc
     2761 gcccgcccgt aggcccgcgt catcgccagg tgaaagcccg tctcgtcggt aaagaccaag
     2821 cggcgacggt cgagccgctc gacgcggcgg cggaaagcct gccggtcggc ttcgacccgc
     2881 tcggaggccg cgaggctttt ttttacgcgg caggtcgagc cgctggagcg cccggcacac
     2941 ggtcgaggga tgcacttgaa cccggcacct ctttcttaag taggccgcca gttcttccag
     3001 cgtggcatcg ggtcggcgct ctccttgcgc ccgcaacagc gccagccgcc tctcatcaag
     3061 cgaggcgggg tagccgccgc cgtgcggcag gggcgcgagc cccgagcctg agcgcgccag
     3121 cttcagtaac cgcccgaccg tacagcgggc gacgccgaag gtctcagcta tctcgtcgaa
     3181 tgtaccctcg ccgccctcgt aggcgagcac gatcttctct cgcaggtcgg tcgagtacgc
     3241 cctcatgggc taatgagtgg cgtaagttgc agtctcaatg caagtgcaaa gcgctataac
     3301 attccacaag gagatcgaaa tgggtgacga cgcggaattt tacatagagc agcaagaaga
     3361 ggaggcacga tttaagcaag cgagcgaata cgcggcgcta gacagcaaaa gaaaacccgt
     3421 tctgtgctgg gcagacggag tcggatatga ggatgaaata tggagttggg agcccatgaa
     3481 caggatactt gggattttct ccaatcttca cgcagagagg cggatcggta gtgacttctt
     3541 cctggccagc cgagttccaa tcgaaggcga tgaagaggag tgggataacg aatatgccag
     3601 ccggcccgaa agcgatgatc gtagggagat caagttcgtt gatgagttgg tgttttacgt
     3661 tgcgaatagt gaagctgatg caacgcacga ggtaatcgtc ttgtctcgat cggatgccga
     3721 cctgttaagg gaggaagttg ttcggcagaa gagcactgcg aaaacttcga aggaaaaaat
     3781 gctcgccgag atgctggaag agatagtttc agttatcgaa gccgactcac agcgtaacgg
     3841 gttcgttttt gcaaggacac tgtgagaagc atctaacact gcgctccaac cgtccgtcga
     3901 cccgatagag ttgctgaagg cggcgggact gaggctgaac cgcgacctct ctgacggcga
     3961 ggacgagggc gaggcgctga tcgcggagtt cgtccgggtg gcaggcatgg cccatacggg
     4021 catcttcatc ggcgactccg tgtacctgcg tgagttcaac aagaaagcga cctgggcctt
     4081 gatgccgcac ctcgtcgcca ccgcgaggac caatgaggac ggcagactca ccctcagggc
     4141 cgcgaagcca ggctcctact acctcatggg cggggcgccg ccaccggccc acgccgtgtg
     4201 gaccatgccg atcacgctcg accgagacca agccgtccgg ctcgacagct cgaacacgat
     4261 ggccgcgtcg cgggcgaacg acgcaaacta atcggcgtgc gccgcaaacc cgcccgcttc
     4321 cgccgtcact aacgcgtcga tgatttgcgt cgaaagcact tccgtgacga ccggctgccg
     4381 cttcggctcg gtgtggcttc tatagaagga acagggatac acggcagatt tcggcgttcc
     4441 ggtcttcgct gacgatgtcc gagtcgtcga actatccggt cttcaaaggc ccggcgacct
     4501 tgtaagcact gaagacgatg ccggacggga atgctttcgt gctgacgagc tcgaatgagc
     4561 gcggcggggt tttctccgca aagcagcgct tccccgtgcc caacaggacc ggatagacga
     4621 ccagcaggac ttcatccgcc agcccatgtt cgagcagcgt cgacgtcagc gtggagctac
     4681 ccgagaggat gaggtccggg ccgtcctgcg acttgacgcg gcgaacgccc tcgacgatgt
     4741 ccggcccaag gccctcgaac gggccccatt cgagactttc cggacggtgg gtagcgacga
     4801 attttgtggc cgcgttgagg ccgtccgcca tcgggctgct cggcgccttc ggccagaagc
     4861 gcgaccagat gtcgtaggtg cgacggccga gcagcaggtc gaagctcccg ccatacgcgg
     4921 cgaggatcgc gtcccggccg gcgggggtgc gataggacgc ggtccagtcg ctgtatggaa
     4981 aatcgtcgtc atcggcggag tgttggatca cgccgtcgag cgagatgtgt tcgatgattt
     5041 tgagccttct cattcgggtc tccgtcttct gtggttggag tttctaatct gcgctgcggc
     5101 cgccttcgtc ctccgtctaa ttctcgcggg caggttcgac gcaccgcggg ctgtcgttcg
     5161 aggggctgtt aaccatcagg cccaccgcgt acgtggtcat ctcctcacgc gggtaggggc
     5221 gcaccagcgg cagtagtcgc tcggcgcttc gcacgccggc gtcgagccac aggtcgtaat
     5281 cctccggcct gaggatggcc ggcatccggt cgtgatagcc cgcgagcagc tcgttcgcct
     5341 cggtcgtgag cagcgtacag gtctccaaca gctcgccgcc tccctcccaa ctctcccaca
     5401 gcccggctac cgcgaaaggc tcgccgtccc gcatgtggaa gtagaacggc cgcttcctgt
     5461 cgcctcgccg ggcccattca tagaacccct ccataggcac caggcaccgc ctgcgcgaga
     5521 aggcatcccg gaacgagggc ttctccgtta ccgtctcggc gcgcgcgttg atgagcttat
     5581 tcccgatgga cgggttcttc gcccagcgcg gcaccagccc ccacttgagg aacgtggcct
     5641 cgcggccgtc ggaagcctcc cggacggcga gcacagtctg tgtcggggcg acgttatagc
     5701 gctcgacgag cggcggcacc tcctcgacct tgaacttctt cgtgatcttc ttcgccggcg
     5761 atctctgggc gaacctcccg cacatggcag ctcctccccg tagaaaagta tagacagata
     5821 tacgttttgc gtattataca tagtttcaag attggcacag aactgaaaac ggagatattc
     5881 ggaattatga cctacgtgct gtggatcatt caggcactgc tcgcgctcct gttcctgttc
     5941 gccggcggga cgaagctggt cataccgccc gacgtgctgg cctcgatggg ctcgcccaac
     6001 cagataccgc tgccggggtg gttcgtgcgc ttcatcggcg tggccgaggt gctgggcgcg
     6061 ctcggactga tcctgccggg gctgctgcgc atcaagccta ggttgacgcc gctggccgcc
     6121 gccgggttga tggtcatcat gatcggcgcg acggctcttg cgatggcggc cgacggggtc
     6181 gcggccggaa tggttccgct tgcggttgga ttgctcgcgg tcttcgtcgc ctacggccgt
     6241 tggcggttgg cgccgcaccc ggcgcggcgc gagatgatcc gtgggaggtc gagtcgagaa
     6301 cgacaagaac aattaaaggg agaatgagat gacgactgag aacagccggg ccgacgacga
     6361 agcccggata cgcgaactca tcgaggaacg ggtcaggacg atccgcgcca aggacgttga
     6421 cgcgctgatg agcaaccacg cgccggacat agtgatgttc gacgcactcg acccgttgcg
     6481 gtacgtcggg tcggaggcgg tcaggaagcg cgccggtcag tggctctctt ggtatcaagg
     6541 gccggtcggc tacgaggtgc g
//
LOCUS       DBNGOH010000346         3051 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3168531, whole genome shotgun sequence.
ACCESSION   DBNGOH010000346 DBNGOH010000000
VERSION     DBNGOH010000346.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3051)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3051)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3051
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3168531"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..349)
                     /locus_tag="AC159P_06800"
     CDS             complement(<1..349)
                     /locus_tag="AC159P_06800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695201.1"
                     /translation="MSGLNMLELLTAAASLATAVGVIVAAYQIRLTRQLSRTQFEDDL
                     TKQFREIIRRIPIEALLGEELSEEAYDRTRDDFFRYIDLSNEQVFLRRNDRVSARTWK
                     LWCEGIKAFLSRPA"
     gene            complement(371..814)
                     /locus_tag="AC159P_06805"
     CDS             complement(371..814)
                     /locus_tag="AC159P_06805"
                     /inference="COORDINATES: protein motif:HMM:NF025210.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HIR3695202.1"
                     /translation="MSTADVHELLRRTKVEVAPETFVVVGLGHADWKRLLENPELSPR
                     AEAVFMLLRDPHEVTLLLEEEDWRTMRHAAREARVESGFRLVTLNVELPWTTVGYLAR
                     VTEILAAAGISVGALSAFSRDHLLVKQDDLARALRALGEHVEELC"
     gene            complement(897..2564)
                     /locus_tag="AC159P_06810"
     CDS             complement(897..2564)
                     /locus_tag="AC159P_06810"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0004534 - 5'-3' RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HIR3695203.1"
                     /translation="MSNVIEIIPLGGVGEFGMNCMGVRCGDDMIVVDAGMGFPEESAY
                     GVDVCVPDFDFLEEYRDNIIAVVLTHGHEDHLGALPYILKKFNVPVYMSHFTAGLAEY
                     KLEEHGLTNDVLMHRVEPRQTVEIGDFEVEFIRVSHSLIDCFALAIKTPAGTIIHTGD
                     YKVDETPVIGEPIDLRTLRRYGQEGVLALTSDSTNATVPGRTPSERAVIPAFEEIFEE
                     TEGRIIVAAFASSIHRLQIVLDVAQQFDRKVCVLGRSMLKNVEIAQRLGYLDIHDMLV
                     PLNEAKRLPDEDIVYLVTGSQGEPRAALAQMSTGSYKGLSIREGDTVVLSARIIPGNE
                     RSISRLIGEIYKRGGNIIDEKRRLIHVSGHASQDDIRIMTEAVRPRFVVPVHGEYRML
                     YRHKEFLKNHCDFPEENIILIENGDVLELGDGRAEVIDKREVKRTFIDESGFEEIDNE
                     TVRERKQLAYDGVVTPVVTLSEETGRLESPPEIAARGLLGLDGDGARNGFLKDMQRVV
                     TEAVENAPAAERRDTSLLKERVRLELKRYIQKQTGARPVIMPVVVQV"
     gene            2826..3038
                     /locus_tag="AC159P_06815"
     CDS             2826..3038
                     /locus_tag="AC159P_06815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695204.1"
                     /translation="MGGRRRMREMDEKVDILLNAQIKNEGLFARNEERFAQLAESQAH
                     SDRKLDALIDIIRQEHEAREGDSEQS"
BASE COUNT          530 a          986 c          968 g          567 t
ORIGIN      
        1 acgcgggccg cgataggaaa gccttgatcc cttcacacca cagcttccac gtccgcgcgc
       61 tgacgcggtc gttccggcgc aggaaaacct gctcgttcga caggtcgatg tagcggaaga
      121 agtcgtcgcg cgtccggtcg taagcctctt cgctcaactc ctcgccgagc agcgcctcga
      181 tgggaatccg cctgatgatt tcgcggaact gcttcgtgag gtcgtcttcg aactgggtgc
      241 gcgagagctg ccgggtcaat ctgatctggt acgcggccac gataacgccg acggcggtcg
      301 ccagcgacgc ggcggctgtc aacagctcaa gcatattaag cccgctcatc ctgccggaag
      361 acttcgaccc ctaacacaac tcctcgacgt gctctcccaa cgcgcgcagc gccctcgcga
      421 ggtcatcctg cttgacgagc aggtggtcgc gcgagaaggc cgagagcgcc ccgaccgaga
      481 tgcccgccgc cgccaatatc tcggtcacgc gcgcgaggta gccgacggtc gtccacggca
      541 gctcgacgtt gagcgtcacg aggcggaagc ccgactcgac ccgcgcctcg cgcgcggcgt
      601 ggcgcatcgt gcgccagtcc tcctcttcga gcagcagcgt cacctcatga gggtcgcgca
      661 gaagcatgaa gacggcttcg gcgcgcggac tcagctccgg gttttcaagc agccgcttcc
      721 agtccgcgtg gccgaggccg acaacgacga acgtctcggg cgcgacctcg accttcgtgc
      781 ggcgcaaaag ttcatgaacg tcggcggtgc tcataaagaa agggagccga agtcagtctg
      841 aagaagacgc gacttcttga ttctgacttc tgactcctga ctttcggact tctctttcag
      901 acctgcacga cgaccggcat gatgacgggc cgcgcgccgg tctgcttctg gatgtagcgc
      961 ttgagttcga ggcggacgcg ctccttgagg aggctcgtgt cgcggcgctc ggcggcgggc
     1021 gcgttctcga ccgcctcggt gacgacgcgc tgcatgtcct tgaggaagcc gttgcgcgcg
     1081 ccgtcgccgt cgagtccgag caggccgcgc gcggcgatct cgggcggcga ttcgagcctg
     1141 ccggtctcct cgctcaaggt gacgaccgga gtcaccacgc cgtcgtaggc gagctgcttg
     1201 cgctcgcgca ccgtctcgtt gtcgatctcc tcgaagcccg actcgtcgat gaaggtgcgc
     1261 ttcacctctc gcttgtcgat gacctcggcg cgcccgtcgc cgagttcgag cacgtcgccg
     1321 ttctcgatga ggatgatgtt ctcctcgggg aagtcgcagt ggttcttcag gaactccttg
     1381 tgacgataaa gcatccggta ttcgccgtgg acggggacga cgaagcgggg cctgacggcc
     1441 tcggtcatga tgcggatgtc gtcctgcgag gcgtggccgc tgacgtgaat caggcgccgc
     1501 ttctcgtcaa tgatgttgcc gccgcgcttg tagatttcgc cgatcagccg cgagatgctg
     1561 cgctcgttgc cgggaatgat gcgggccgac agcaccaccg tgtcgccctc gcggatggag
     1621 aggcccttgt aggagccggt ggacatctgc gcgagcgccg cgcgcggttc gccctgcgaa
     1681 cccgtcacga ggtagacgat atcttcgtcg ggcagccgct tcgcctcgtt caaaggcacc
     1741 agcatgtcgt ggatgtcgag gtagccgagc cgctgggcga tctccacgtt cttcagcatc
     1801 gagcgcccga gcacgcagac cttgcggtcg aactgctgcg ccacgtcgag cacgatctgc
     1861 aagcggtgga tcgaagaggc gaacgccgcg acgatgatgc gcccctcggt ctcctcgaaa
     1921 atctcctcga aggctgggat cacggcgcgc tccgacggcg tgcgccccgg cacggtggcg
     1981 ttggtcgaat cggacgttaa ggcgagcacg ccctcctgcc cgtagcggcg cagcgtcctg
     2041 aggtcgatag gctcgccgat gacgggcgtc tcgtccacct tgtagtcgcc ggtgtggatg
     2101 atggtgccgg cgggcgtctt gatggcgagc gcgaagcagt cgatcaaaga gtgcgagacg
     2161 cggatgaact cgacctcgaa gtcgccgatc tccacggtct gccgcggctc gacgcggtgc
     2221 atcagcacgt cgttcgtgag gccgtgctct tcgagcttgt actcggctag ccctgcggtg
     2281 aagtgcgaca tgtagacggg gacgttgaac ttcttcagga tgtaggggag cgcgccgagg
     2341 tgatcctcgt gcccgtgcgt gaggacgacg gcgatgatgt tgtcgcggta ctcttcgagg
     2401 aagtcgaagt ccggcacgca cacgtccacg ccgtaggcgc tctcttccgg aaagcccatc
     2461 ccggcgtcca cgacgatcat gtcatcgccg cagcgcacgc ccatgcagtt catcccgaac
     2521 tcgccgactc cgccgagcgg gattatctcg attacgttgc tcactttttt tgctttctct
     2581 ccaaaccgga cggcactgcg ggccgcctca ctcacagggg ggtttttgta atcccggagt
     2641 ataacacacg cccccacgct ttcaccgcca ccgctgcgcg ctctgtccga tcaattgcgc
     2701 gtgtgctaac ctttccgcca ccgccctgca atgtcaacac acattggagg ccaagaccct
     2761 tgatgacgcc ggaacagaga ctcgaccgct tagagcgcat cgccaagcta ctcgtcaggg
     2821 ccggcatggg cggtcgccgc cgcatgcgcg agatggacga gaaggtcgac atccttctca
     2881 acgcccagat caagaatgaa ggacttttcg cgcgaaacga ggagcggttc gcgcagctcg
     2941 ccgagtcgca ggcgcattcg gatcgcaagc tcgacgcgct gatcgatatt attcgccaag
     3001 agcacgaagc gcgcgaaggc gactccgaac agtcgtagcg gccttcgctc g
//
LOCUS       DBNGOH010000347         2851 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1336797, whole genome shotgun sequence.
ACCESSION   DBNGOH010000347 DBNGOH010000000
VERSION     DBNGOH010000347.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2851)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2851)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2851
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1336797"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            463..750
                     /locus_tag="AC159P_06820"
     CDS             463..750
                     /locus_tag="AC159P_06820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695205.1"
                     /translation="MGSVRFAPPVERRWKVSGQQVSRAPETCARCAGTGRWAVSAAYV
                     ISCLVCGGKGKVMTAQPAAPCRDCEGSGRRGATAMCLTCAGTGWGRVFDQG"
     gene            983..1258
                     /gene="rpoZ"
                     /locus_tag="AC159P_06825"
     CDS             983..1258
                     /gene="rpoZ"
                     /locus_tag="AC159P_06825"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF013365.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HIR3695206.1"
                     /translation="MAETDVIAGGGVGRAADDEEWPGIDSRFRLIIVAALRSKQLLHG
                     SRPRVAADPSKRRNTSIALEEVRQGLVTFTITDGDRKGADGDDGRAI"
     gene            1344..1640
                     /locus_tag="AC159P_06830"
     CDS             1344..1640
                     /locus_tag="AC159P_06830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695207.1"
                     /translation="MKFTAEHFEQARRVAERWRQDGYNHVVVVRDGARAKRCYPCRQQ
                     DVPPRTVASWDYYPIPTLDRLNKSAPAEQSASRAELWIAATILYGCRYAAAARN"
     gene            1689..1844
                     /locus_tag="AC159P_06835"
     CDS             1689..1844
                     /locus_tag="AC159P_06835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695208.1"
                     /translation="MPHRYCPACAYELRRLRLRCPDCRRSTISWLHVALAAAFDAAGL
                     VYLLKLV"
     gene            1931..2314
                     /locus_tag="AC159P_06840"
     CDS             1931..2314
                     /locus_tag="AC159P_06840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695209.1"
                     /translation="MTTSYRAEKPVAVRFVDAGPGVLLELTNTTDQALKGVEILTVFL
                     KDEETPGGPSRAHIRFEAVKNIQPGGKAVLSHKTLIDGKTAAPAQDQLERLKVIAGEI
                     KPYVLDISWEDADGKSQFLRIPVGH"
     gene            2354..2596
                     /locus_tag="AC159P_06845"
     CDS             2354..2596
                     /locus_tag="AC159P_06845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695210.1"
                     /translation="MIVCEFCMRYGADGKCGLGLNLPKGMGCREFDPGMERFCSDPKD
                     FVSPRQIIGMATHFGIKGMELKKVKAMTAREASNRS"
BASE COUNT          510 a          875 c          978 g          488 t
ORIGIN      
        1 cccactggga cgccctaacc gcttttgtgt gccgatggtg tgccgaattt cagcgtatct
       61 ttttcttgcg cgcgaacgac ctgctttccg gcttatcgct cgccgcctgc cgtgtaactc
      121 ggtagcacgt gcgagtaggt gtcgggcatc agcgtggtgc cggcgtgccc agccgctcgc
      181 cggcaacccc ggggcgttcg ttcgcggcca gcatagggcg ttcattcgtg cccgtggtat
      241 acttcttcct gagttagcga ccgctcttta gaaaatacaa ctctcgacat caggattggc
      301 gcacgccgct ctgctcctag tgccgtctgc tgttttccga acgcgaccac tacggaggcc
      361 cctgatgccc gcaccagtac caaccgacaa ttcaagtgac agccgtcctc cgggtctgcc
      421 gacctgtccg gctcgagcac gccgtccggg gccgaggcgg ggatggggtc tgtccgtttc
      481 gccccacccg tcgaacggcg gtggaaggtg agcgggcagc aggtcagtcg cgcgcccgag
      541 acttgtgcgc ggtgcgcggg aacggggagg tgggccgtct ccgcggccta cgtcatctcg
      601 tgtctggtgt gcggcggcaa gggcaaggtc atgacggccc agcccgcggc gccctgccgc
      661 gattgtgagg gcagcggcag gcgcggggcg accgcgatgt gcctgacttg cgcggggacg
      721 gggtgggggc gcgtgttcga tcagggatag gccgatcgtg acgaagagac gctgccgcta
      781 ctgcgggacg aggatgcggc ggcgaaggtg gcgctgccgc tactgccgtc ggtcggctgt
      841 gagccggctg cactgcgggg tcgccgccgc cttcgtactg atcgcagccg ccctttatct
      901 gatgaaaacg ttttagtcca ctccgaagtc aggctcggtc cggaatgccg gcctcagcgg
      961 aacgtggtga caggagtagc cgatggctga gacagatgtg atcgcgggcg gcggggtcgg
     1021 gagagccgcg gacgacgaag agtggccggg aatagattcc cgattcaggc tcatcatcgt
     1081 ggcggcttta agaagtaagc aactgctgca cggctcgcgg ccccgcgtcg ccgccgaccc
     1141 gtcgaagcgg aggaacacga gcatcgccct cgaagaggtc agacagggtc tcgttacttt
     1201 cacgatcacc gacggcgacc ggaaaggcgc tgacggcgac gacgggcggg ccatttgaaa
     1261 cacgacgcat aggccgccgc gcagtcgagt tgacgatgga ggatggatga ttcgcttcgg
     1321 cgtaacagcc ggaaaggtgg cgcatgaaat tcacggcgga gcacttcgag caggcccgga
     1381 gggtggccga gcgctggcgg caggacgggt acaaccacgt cgtggtggtc agggacggcg
     1441 cgcgcgccaa gcgctgttac ccgtgccggc agcaggacgt gccgccgcgc accgtggcga
     1501 gttgggacta ttacccgatc ccgacattgg acaggctcaa caagagcgcc cccgcggagc
     1561 agtccgcgtc gagggccgaa ctgtggatcg ccgccaccat cctgtacggg tgcaggtacg
     1621 cagccgccgc gaggaactga ggggcccgcc gcccgcgcag gaaggccgga gaggggaagg
     1681 taacgcatat gccgcaccga tactgcccgg cttgtgctta cgaattgcgc cggctacgtc
     1741 tgcgctgccc cgactgccgc cggtctacca tctcctggct gcacgtcgcg ctcgccgccg
     1801 ccttcgacgc ggcgggactc gtctacctgc tcaaactcgt ctagccgggc gagacggctt
     1861 gtgcgcggcg ccgaatcgga ggcggcgccg taacaatgcg ttcgtaagtt aaagacgcgg
     1921 gggaaagatc atgaccacca gctacagggc ggagaagccc gtcgccgtca ggttcgtgga
     1981 cgcggggccg ggagtgctct tggagttgac gaacaccacc gaccaggcgc tcaagggcgt
     2041 cgagattctg accgtcttcc tcaaagacga agagacgccg ggcgggcctt cgcgcgcgca
     2101 catacgattc gaggccgtca agaacataca gccgggaggc aaggccgtcc tgtcacacaa
     2161 aactctgatc gacggcaaga cggcggcccc cgcccaggat cagttggaac gtcttaaagt
     2221 gatagcgggc gaaatcaaac cttacgtgct ggacatctct tgggaggacg cggacggtaa
     2281 atcccaattt ctaaggatac ccgtcgggca ttgacgggcc gggcgcgggt tatgctgcgg
     2341 aaggaatcgc gtgatgatag tttgtgaatt ctgtatgcgg tacggggcgg atgggaaatg
     2401 cgggctgggc cttaacctgc cgaaggggat gggctgccgc gagttcgacc ccggcatgga
     2461 gaggttctgc tccgacccga aagatttcgt cagcccgcga cagatcatcg ggatggcaac
     2521 ccacttcggc atcaaaggaa tggagctgaa gaaggtcaag gccatgacgg cgcgggaagc
     2581 aagtaatcgg tcttagctcg gcgccgggcg cggcctccgg cggcggcggc gcgtgccgca
     2641 cgtacgcacc gcgctatact cccggcgacg ctccttcagc gcagttcctt ctcgaactct
     2701 tccacttcct tgcggtagac gaggtcgcgc ccctccacct cgaccgagcg gaggcgaccg
     2761 cggttcacca tctcgtggat cgcggcgcgc gtcatgccgc acatctccgc cgccttggcc
     2821 tgactgatga gcggttcgtt cgccgcgtca t
//
LOCUS       DBNGOH010000348         2027 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1153917, whole genome shotgun sequence.
ACCESSION   DBNGOH010000348 DBNGOH010000000
VERSION     DBNGOH010000348.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2027)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2027)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2027
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1153917"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..389
                     /locus_tag="AC159P_06850"
     CDS             <1..389
                     /locus_tag="AC159P_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_245918803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS701 family transposase"
                     /protein_id="HIR3695211.1"
                     /translation="RLSCGAGSKGERVYDWARVPLREVEGGRARWLLTRRSLSEAQEV
                     SYYVASGPRSLSLEELARVAGARWAVEESFETAKGEVGLDQYEVRSWHGWYRHITLCL
                     LAHAYLTVTRSRAAAVEEAAQKSGHA"
     gene            complement(954..1124)
                     /locus_tag="AC159P_06855"
     CDS             complement(954..1124)
                     /locus_tag="AC159P_06855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695212.1"
                     /translation="MKTGTISGAADGARNQEAADTASLLRREEHVARSEAVGGLQRLF
                     QTLFRKSEKAIR"
     gene            complement(1143..>2027)
                     /locus_tag="AC159P_06860"
     CDS             complement(1143..>2027)
                     /locus_tag="AC159P_06860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695213.1"
                     /translation="ARYRLRQFAPAERLLAALVETFREWGGAGVPNVAILDWADLPTA
                     DEFVLLRDFFAGRGVPTVICAPEELEYANGRLRRGDFPVDLVYKRVIIHELLARADDS
                     HPLVRAYLNKDVCLVNSFRCKMLHKKAAFELLTDGSREGWFTPDEREVIRRAVPWTRR
                     VAERKTIYRGREIDLIEYVRKNRRAFVLKPNDDYGGSGVSLGDGASESEWDAALAAAL
                     GGDYVVQEKVALRTEVFPVFGERGWALQPMYVDTNPFLFRGKVEGAMVRLSDSPVVNV
                     TSGGGETGFYVIDGEVAG"
BASE COUNT          352 a          639 c          699 g          337 t
ORIGIN      
        1 ggcggctgag ttgcggggcg ggcagcaaag gcgagagagt ctacgactgg gcgcgggtgc
       61 cgttgcgcga ggttgaaggt gggagggcgc gatggctgct gacccggcgc agcctcagtg
      121 aggcgcagga ggtctcttat tacgtcgctt cggggcctcg cagcctctcg cttgaggagt
      181 tggcacgggt ggcaggggcg cggtgggcgg tcgaggagag cttcgagacg gcgaagggcg
      241 aggtggggct cgaccagtat gaggtgcgga gttggcacgg ctggtatcgg cacatcacgc
      301 tttgcttgct ggcgcacgcc tacctgacgg tgacgcgctc gcgtgcggca gcagtcgaag
      361 aagcggcgca aaaaagcggg cacgcctaac aatacgcgtg ggcaggcagc ggagttgatc
      421 ccgctgacag tgcccgaggt aagacgactg ctatggtggc tggtgtgggg aaggatgccg
      481 acacacgagc aggttctcag ttggtcagag tggcgacgca gacatcaggc aggagccaag
      541 cgttgccact acaaacgcag agcagcacat ctacaactgt agtactaaga gcgcggcgaa
      601 gtcagccatg atgttcctgc tgagaaggta gacagaggtt caccggaacg tgctggcttc
      661 agccctgcga gctggttata gtagggccgc ccggcggcag catccgagcg ttcgttgtcg
      721 ggccaaaggc ggggcgcgcg gactggttgt tagaagcgtc tcgatcacgc tacggcatgc
      781 aggttcgtcg cgcccgccct gcatgttctt ccctcgctct ccactctaat gaagcagagc
      841 cgaagaatga acctcgacaa cctataacgg caggcacccg accgcaatca cgtagccgct
      901 caagcccgaa ggcagcagcg ggcgggtgtt gcagatcgtt agcccctcca cttttaccta
      961 attgccttct cagactttct gaaaagggtc tggaagaggc gctggagtcc gccgacggcc
     1021 tcgctccggg cgacgtgttc ctcgcggcgg agcaggctcg cggtgtcggc ggcctcctga
     1081 tttcgtgcgc cgtcggcggc gccgctgatc gtgccggtct tcacggcggt gatgtacgac
     1141 cgttagccgg cgacctcgcc gtcgatgacg tagaagccgg tctcgccgcc gcccgacgtg
     1201 acgttgacga ccggcgagtc cgacagccgg accatcgcgc cctcgacctt gccgcggaag
     1261 aggaaggggt tcgtgtcaac gtacatcggc tgcaaggccc agccgcgctc gccgaagacg
     1321 gggaacacct ccgtccgtag cgcgaccttc tcctgcacga cgtagtcgcc tccgagcgcc
     1381 gccgccagcg ccgcgtccca ctccgactcg ctcgccccgt cgccgagcga gacgccgctc
     1441 ccgccgtagt cgtcgttggg cttcaggacg aacgcccgcc ggttcttccg cacgtattcg
     1501 atcaagtcta tctcacgccc ccggtagata gtcttcctct ccgcgacgcg ccgcgtccac
     1561 ggcaccgcgc ggcggatgac ttccctctcg tccggcgtga accagccctc gcggctcccg
     1621 tccgtcagca actcgaacgc ggccttcttg tggagcatct tgcagcggaa ggaattgacg
     1681 aggcacacgt ctttgttgag gtaggcgcgg acgagcgggt gcgagtcgtc cgcgcgcgcg
     1741 agcagctcgt ggatgatgac gcgcttgtaa accagatcga cggggaagtc gccgcggcgc
     1801 agtctgccgt tcgcgtactc cagctcttcg ggggcgcaga tgacggtcgg cacgccgcgc
     1861 ccggcgaaga agtcgcgcag caggacgaac tcgtcggcgg tcggcaggtc ggcccagtcg
     1921 aggatggcga cgttcggcac gcccgccccg ccccactcgc ggaaagtctc gacgagcgcg
     1981 gccagcagac gctccgccgg cgcgaactgc cgcaggcggt agcgggc
//
LOCUS       DBNGOH010000349         3169 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4818494, whole genome shotgun sequence.
ACCESSION   DBNGOH010000349 DBNGOH010000000
VERSION     DBNGOH010000349.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3169)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3169)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3169
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4818494"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1468)
                     /locus_tag="AC159P_06865"
     CDS             complement(<1..1468)
                     /locus_tag="AC159P_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020464771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - modified amino acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-cysteine ligase family protein"
                     /protein_id="HIR3695214.1"
                     /translation="MGEHQIEAGCDEGKVRAFTRALLNDLAALERMLDGGLIESGVRR
                     IGAEQEMFLIDRHLRPAFVAEEVLSRAGDPRLTTEIARFNLEANLTPRLLAGDCLRRM
                     HEEADEVVAAVRAGARECGADVLLAGILPTLRRGDLGLGSITPSPRYHQLNDAIMRMR
                     GGSLNVHIKGLEEVNVVSDNIMPISSTTSFQVHLQVGPEEFARLYNTAQAVTAPVLAA
                     AVNSPIWLGNRLWQETRLALFQHSVDERSSTRVARQFPTRVGFGEGWLRGSVLELFRE
                     DIARFRVIMTNEADEDPAEVLARGGVPRLKALTLHNGTVWWWNRPCYGVHEGRAHLRI
                     ENRVLPAGPTVLDEVANAAFFVGLMRALPEAYGEIDKLLAFDDAKANFLSAARHGLDA
                     RLAWVGGRTRSAVALILEDLLPLARAGLKASKIDETDADLYLGTVEERVRTGQTGAQW
                     ALRSLAALGAEGTREGRMRTLVAAMRERQRTGEPVHRWP"
     gene            1637..2287
                     /locus_tag="AC159P_06870"
     CDS             1637..2287
                     /locus_tag="AC159P_06870"
                     /inference="COORDINATES: protein motif:HMM:NF013936.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HIR3695215.1"
                     /translation="MTLDTRTATSVTANDACVVFGRKTEEIVRSLEEASRLEARVGAL
                     RGPDSASLTKFIGYLIDKHHVYARRQAAAIAALLARLSAAHEQCRPELARVRSLFEVL
                     RRELLFHMEKEEVSLFPRIIQTEAAATRGEPREAAHTRSARDPIRMSMDEHDGLCAML
                     DEIRAATNDYAPPRGACADYRTLCRALRDLELDLLHLIYLEDRVLFPRALEMERRA"
     gene            2724..>3169
                     /locus_tag="AC159P_06875"
     CDS             2724..>3169
                     /locus_tag="AC159P_06875"
                     /inference="COORDINATES: protein motif:HMM:NF012257.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HIR3695216.1"
                     /translation="MDSITSAVENARGDVVFVEGQQPGGVYVVCRGHVKLSTSSGDAR
                     VLITDIAEPGDVLGLSAAVSGRPYEVTAEALGNCRLSFIRREDFLRLLDEHAGAARRA
                     TRQLSRDYHTAHRQARLLGLSASAAGKLAGVLLEFCERGGRPTERG"
BASE COUNT          481 a         1144 c         1062 g          482 t
ORIGIN      
        1 gcggccaacg gtggacgggt tcgcccgtcc gctgccgctc gcgcatcgcc gcgacgagcg
       61 tgcgcatccg gccctcgcgt gtgccttcgg cgccgagcgc ggcgagcgag cgcaaggccc
      121 actgcgcccc ggtctgcccc gtgcgcacgc gctcctcgac ggtgccgagg tagaggtctg
      181 cgtccgtctc gtcgatcttc gacgccttga gacccgcgcg cgccagcggc aggaggtctt
      241 cgaggatgag agcgacggcg gagcgggttc ggcccccgac ccacgcgagc cgcgcgtcga
      301 ggccgtggcg cgcggcggag aggaagttcg ccttcgcgtc gtcgaacgcg agcagcttgt
      361 cgatctcgcc gtacgcttcg gggagcgcgc gcatcagccc gacgaagaac gcggcgttcg
      421 ccacctcgtc gagcacggtc ggccccgccg gcaggacgcg gttctcgatg cgcaggtgcg
      481 cgcggccctc gtgcacgccg tagcaggggc ggttccacca ccacaccgtc ccgttgtgca
      541 gcgtcagcgc cttcaaccgc gggacgccgc cgcgggccag cacctccgcc gggtcttcgt
      601 ccgcctcgtt cgtcatgatg acgcggaagc gcgcgatgtc ctcgcggaag agttcgagca
      661 cggagccgcg cagccacccc tcgccgaagc cgacgcgcgt cgggaactgc cgcgcgacgc
      721 gcgtcgaaga gcgctcgtcc accgaatgct ggaagagcgc gagccgcgtc tcctgccaga
      781 ggcggttgcc gagccagatc ggcgagttga ccgccgcggc gagcaccggc gccgtcaccg
      841 cctgcgccgt gttgtagagc cgcgcgaact cctcggggcc gacttgcagg tgcacctgaa
      901 agctcgtggt cgaggagatc ggcatgatgt tgtcggagac gacgttgacc tcttccagcc
      961 ccttgatgtg cacgttgagc gagccgccgc gcatccgcat aatggcgtcg ttcaactggt
     1021 ggtagcgcgg gctgggcgtg atgctgccga ggccgaggtc gcccctgcgc aaggtcggca
     1081 gtatccccgc gagcagcacg tcggccccgc actcgcgcgc gcccgcgcgc accgccgcca
     1141 caacctcgtc cgcctcttcg tgcatccgcc gcagacagtc gcccgcgagc agccgcggcg
     1201 tgaggttggc ttcgaggttg aagcgcgcga tctcggtggt cagacgcgga tcgcccgccc
     1261 gcgacagaac ctcctcggcg acgaaggcgg ggcgcaggtg ccggtcgatg aggaacatct
     1321 cctgctccgc gccgatgcgc cgcacaccgc tctcgatcag cccgccgtcg agcatccttt
     1381 cgagcgccgc cagatcgttc agcagcgccc tcgtgaacgc gcgcaccttg ccctcgtcgc
     1441 agcccgcttc gatttgatgt tcacccataa ctttgtctta catgcccggc ccttgtcgct
     1501 gtcgatttgc gtcgggtaga ggtctcgcgt ctctctgcgc cgctctggaa agcaaattgc
     1561 attcccgtct gtcgtgagtc gcgccgcgcg gtatcgagag gcgcgacttc ggaaaccgca
     1621 catgaggggg aaggttatga cactcgacac ccggacggca acatcggtca cggcgaacga
     1681 cgcctgcgtg gtcttcgggc ggaagacgga agagatcgtc cgctcgctcg aagaggccag
     1741 ccggcttgag gcgcgggtcg gcgcattgcg cgggcctgac tcggcctccc tcacgaagtt
     1801 catcggctac ctcatagaca agcaccacgt ctacgcgcgg cggcaggcgg cggccatcgc
     1861 cgcgctgctc gcccggctct ccgccgcgca cgagcagtgc cggccggagc tggcccgcgt
     1921 ccgctctctc ttcgaagtgc tgcggcggga actcctcttc cacatggaga aagaggaggt
     1981 ctcgctcttc ccgcgcatca tccaaacaga ggccgcggcc acgcgcggcg agccgcggga
     2041 agccgcccac acgcgttcgg cgcgagaccc gatacggatg tcgatggacg aacacgacgg
     2101 gctgtgcgcg atgctcgacg aaatccgcgc ggcgaccaac gactacgcgc cgccgcgcgg
     2161 cgcctgcgcc gactaccgca cgctctgtcg cgcgctgaga gaccttgagc tagacctcct
     2221 gcacctcatc tacctcgaag accgcgtcct cttcccgcgc gctctggaaa tggaacgccg
     2281 cgcgtgaacg ggcgagcatg aagcccaccg gcgcccgtgc ctccccctcc ctaacggacg
     2341 cggataaaat attcgaagac cttcacacgg cccgcctccc gctgccttcg ccgcctcccg
     2401 cgcgcggaaa tttccccatc accgtcgcca cgaccgcgag agattccgca gcggtggtgg
     2461 atttttggcc gccgtcaacc ggcgccacgc tttatccggc cacatcgcct gtcctggccg
     2521 cggcacagac cttgccgtcg gacaggcgtt gaggtcacgc ggcagccgac agacaatccc
     2581 gtccgtgctc gcgccccgcg ccctccggag gcggtttgcg aaggacgatg atcgacgaag
     2641 cgggaaacgg ggggcactgt tcgaagtgcg agctgcgggg agacaagttc ttctgcggtc
     2701 tggcggagga cgccctgcgt cggatggatt ccatcacgtc ggcggtcgag aacgcgcggg
     2761 gcgacgtggt cttcgtcgaa gggcaacagc ccggcggcgt ctacgtcgtc tgccgcggcc
     2821 acgtcaagct ctcgacctcc tcgggcgacg cgcgcgtgct catcacggac atcgcggagc
     2881 cgggcgacgt gctcggcctc agcgccgccg tctcgggccg cccctacgag gtgacggcgg
     2941 aggcgctcgg caactgccgt ctctccttca tcaggcgcga agacttcctg cggctgctgg
     3001 acgaacacgc cggcgcggcg cggcgcgcga cgcggcaact gagccgcgac taccacacgg
     3061 cgcacaggca ggcccgcttg ctcggcctct cggcctctgc ggcgggcaag ctggccggcg
     3121 tgctcctcga attctgcgag cgcggcggca ggccgaccga gcgcggcat
//
LOCUS       DBNGOH010000350         8104 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2069256, whole genome shotgun sequence.
ACCESSION   DBNGOH010000350 DBNGOH010000000
VERSION     DBNGOH010000350.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8104)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8104)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8104
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2069256"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            167..2173
                     /locus_tag="AC159P_06880"
     CDS             167..2173
                     /locus_tag="AC159P_06880"
                     /inference="COORDINATES: protein motif:HMM:NF044870.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HIR3695217.1"
                     /translation="MNLNISPTLKACVRALVLLAGCASAASAQIGGPRDTQPKPATTP
                     VAASKPAPTSAAGTFEKEGVRVAFSAKADEQGRRGLVAGADAVVTFSVTDARTGQPLA
                     GVRPRAWISTRKPEHAPSEEECKDKIRTFMGGLLSARPDLDLNSYLLFTLNHDNTISV
                     INPLVSFSRTQLENLITLPGAGADWALSANRDLLYVTLPERAEVAVVSTITRKVVATV
                     PVGEKTRPMRVAVQPGGRYVWVGLDNSPAVAVIDAATNKLAATVEAGAGLHNIMFTPD
                     GQFAYVTNSAADTVSAIDTKKLSKLADIPVGKTPVPVAYSAASRLVYTAAINGSSVSV
                     IDPARQQIVKTIPVGRGVVALRFDPTGRFGFAVNQVENVVAVIDSSTNEVAGRVEVVK
                     GPDQVVFTDRYAYVRGTGSEKFSLVELSGISKGKFAAVDVTAGQKPASALPAEIGVAD
                     MIATTPEGNAAMIANTPDGMIYYYVEGMMAPMGTLSNYKRRPHAAMILDRSLAEVAPG
                     VYTVPVRLKGAGRFDVSVLTGQPRIAHCFQLEVADSPDAEKQSTGVSLAIKALFDGKQ
                     YKAGESVPLRFRLTDSTTGQPLKGLKDVQVLAFEPPGIWQQRQWATEVGEGVYEVSQT
                     FPRPGLYKVMLRSASRGAAFADLPYTSVPVTSGAETVEKDNTRN"
     gene            2200..2499
                     /locus_tag="AC159P_06885"
     CDS             2200..2499
                     /locus_tag="AC159P_06885"
                     /inference="COORDINATES: protein motif:HMM:NF039593.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HIR3695218.1"
                     /translation="MKRTIVTALASVALLAFVSTQAASTNTQQTEQTATPRTGQKTTK
                     PVQTSCPMHPEIKTRSAGKCPKCRMEARKQSAARAKSNGKVQPQPQQQEGAEAND"
     gene            2492..3790
                     /locus_tag="AC159P_06890"
     CDS             2492..3790
                     /locus_tag="AC159P_06890"
                     /inference="COORDINATES: protein motif:HMM:NF014667.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HIR3695219.1"
                     /translation="MINRNTAALALFAAHLLAPAFAGGGGHAHARQQKHEHKSQPATP
                     ARKTVTRYACPMHPDVVSDAPGSCPECGMFLKAADGEKAAPAKISESAAAAAPARPTE
                     PVAPGDAPQIPDLSVYDQQGRRLKFYTDLVKGKTVAINFVFTTCTTICPPLSATFRKV
                     QQELGERVGKDVELISISVDPATDTPERLKGFAEKFQAGPGWTFVTGGKPEIDSLLKA
                     LGAATPDKNDHTPMILVGNEAAGKWTRTYGLAPAATLVKVIGEAAEAPTLSGASAGYF
                     PNHVLVTQDNKPVRFYDDLVKGKVVLINFMFTTCTGVCPPMATNMAKVQSYLGDRIGR
                     DVVILSISVDPTVDTPDKLKRYADSFKARPGWYFLTGEKQNVDWVLYKLGGYVEDKMK
                     HSGVLIIGNESTGEWVKTAALRNPAQIAEAVTALLKTEKK"
     gene            complement(3962..5356)
                     /locus_tag="AC159P_06895"
     CDS             complement(3962..5356)
                     /locus_tag="AC159P_06895"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006869 - lipid transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spinster family MFS transporter"
                     /protein_id="HIR3695220.1"
                     /translation="MKPERASAHFALWVLFAINAMNFFDRQILGAVGEAVRKEWGMSD
                     GAMGALGTAFTLLYAVVGLPLGHLADKTGRKWILSAGVFVWSLTTVASGFARNFWQLF
                     ALRLGVGVGEASCAPAATSLIGDLFPASRRAWALSIFMLGLPVGIALSFAVSGTIARS
                     YGWRSAFYVAGIPGLLCAAAVLLIREPRRGAAELHKVGGRRREGSAYRLVLGTPTMRW
                     LIISGALHNFNMYAIGSFLTPYLMRYHGMDIRSANFVSMAVYGLSGAPGLLLGGLLGD
                     AVVRHRTNGRLLVGALAILGSVPFLFLALGSAPPETVRFMALMGTGCALMYGYYSTVY
                     STIQDVTEPSLRGTAMALYFLAMYVLGASLGPYGTGLISDYFTGRAATAAGVTSLTQQ
                     ALEPFRAEGLHNAMYVIPALGVLLTLVLFAASRTVTKDVEKLQHWMRESAAAEPPAET
                     ARVEAAGARAAEKI"
     gene            5631..8006
                     /locus_tag="AC159P_06900"
     CDS             5631..8006
                     /locus_tag="AC159P_06900"
                     /inference="COORDINATES: protein motif:HMM:NF013188.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter domain-containing
                     protein"
                     /protein_id="HIR3695221.1"
                     /translation="MHVFVAAGTTPPFLIQTVALIAAGALIAYVCQRLRLVPIVGFLL
                     AGVLIGPNALGLVGDRELVDAAAEVGVILLLFTIGIEFSLDRLARIKKLIFGGGGLQV
                     GLATLLTTGLLALFGVDWRAALFTGFLIALSSTAIVLKVLADKSETNHPHGQVSLGLL
                     IFQDLAIIFMVLLVPMLGGGGGSALDIVWAVGKAGAIIAAVLVVARRFMPKLLEAVAR
                     TCSEEIFLLSVIAICFGTAYLTSLAGVSLSLGAFLAGLLVSESRFSEHAFSEILPLQI
                     LFSAAFFISVGMLLDVGFLVRNLPLVAAAVALVLAVKFLTTGASVLALGYKLPVAAAS
                     GLVLAQVGEFSFVLERAGRDAGLSPAGLGAAGSQTFIASTVVLMVATPLLMQAGSKLA
                     RSIESKRAEREGARAEETLPPEHLAELEDHVVVAGYGQAARRLVRVLDGSRIPYLITT
                     LSPEGANEAEAEGLPVLRGDASKRRTLELAGVERAKMLVVADDEPSMARRVTAVARGL
                     NPTARIVVRTRYLAEVERLREAGADRVVADELESIVALFSDVLRDYRIRAEEIDAHEE
                     AIRRGDYAALLQDRAEGEQPAVVCSPGADCFDTRTATVREGAPVTRMSLTELSLGDHG
                     VELREVRRNGELIEDVSEDFVPWAGDELVLVGQPDAFARIASLFRADDGEPPVKHDEI
                     KFEDNGTSRALVDTERVVTLDVENASANCSHLGEVRPVMPRARGCEECLRTSDEWVHL
                     RLCMTCGHVGCCDTSKNKHASKHYPATGHPVIKSLEPGEDWAWCYPDETYL"
BASE COUNT         1395 a         2798 c         2702 g         1209 t
ORIGIN      
        1 cgcccgcgcc gaccgggaac gacccgagac gaatcgataa ttattcagac cctgatggcc
       61 gcccgacggc gagccgaaag acgattcgaa acatcgaatg cgttctctcg aaagttcaca
      121 cggtcaaccc ttcatctcgt cactacgaat cacggacggt gtgtttatga acctgaatat
      181 ctctccgacc ctcaaggcgt gtgtgcgcgc tctggtcttg ctcgcgggtt gcgcctcggc
      241 ggcgagcgcg cagatcggcg ggccgcggga cacgcagccg aagcccgcca ccacgccggt
      301 cgccgcgtcg aaaccggcac cgacttccgc cgcggggact ttcgagaagg aaggcgtccg
      361 cgtcgctttc tccgcgaagg cggacgagca gggcaggcgc gggctggtcg cgggtgcgga
      421 cgcggtcgtc accttcagcg tcacggacgc gcgcacgggg cagccgctcg ccggggtgcg
      481 accgcgcgcg tggatcagca cgcgcaagcc ggagcacgcg ccgagcgagg aagagtgcaa
      541 ggacaagatt cgcaccttca tgggcggtct gctgagcgcg cgacccgacc tcgacctcaa
      601 cagctacctg ctgttcacgc tcaaccacga caacaccatc tcggtcatca acccgctcgt
      661 ctccttcagc cgcacgcagc tcgaaaacct catcacgctg cccggcgcgg gcgccgactg
      721 ggcgctctcg gcgaacaggg acctgctcta cgtcacgctg cccgagcgcg ccgaggtggc
      781 cgtcgtcagc accatcacgc gcaaggtggt cgccaccgtc cccgtcggcg agaagacgcg
      841 gcccatgcgc gtcgccgtcc agcccggcgg tcgctacgtc tgggtcggcc tcgacaactc
      901 gcccgccgtc gccgtcatcg acgccgcgac caacaagctc gccgccaccg tcgaagcggg
      961 cgcggggctg cacaacatca tgttcacgcc cgacggccag ttcgcctacg tgaccaacag
     1021 cgcggcggac accgtctcgg ccatcgacac gaagaagctc tcgaagctcg cggacatccc
     1081 cgtcggcaag acgcccgtgc ccgtggccta cagcgccgcg agccgcctcg tctacacggc
     1141 ggccatcaac ggctcctcgg tcagcgtcat cgacccggcc cgccagcaga tcgtcaagac
     1201 catcccggtg gggcgcggcg tcgtcgcgct gcgcttcgac ccgacgggcc gcttcggctt
     1261 cgccgtcaat caggtcgaga acgtcgtcgc cgtcatcgac tcttcgacca acgaagtcgc
     1321 cggacgggtc gaggtcgtga aggggccgga tcaggtcgtc tttaccgacc gctacgccta
     1381 tgtccgcggc accggctccg agaagttctc gctcgtcgag ttgagcggca tctccaaggg
     1441 caagttcgcc gccgtcgatg tcacggcggg gcagaagccg gcgagcgcgc tgcccgccga
     1501 gatcggcgtc gccgacatga tcgcgacgac gcccgagggc aacgccgcga tgatcgccaa
     1561 cacgccggac gggatgatct attactacgt cgaggggatg atggcgccga tgggcacgct
     1621 ctcgaactac aagcgccgcc cgcacgccgc gatgatcctc gaccgcagcc tcgcggaggt
     1681 cgcgcccggc gtctacacgg tgcccgtccg cctcaagggc gcggggcgct tcgacgtgtc
     1741 agtgctcacg ggccagccgc gcatcgcgca ctgcttccag cttgaggtcg ccgactcgcc
     1801 cgacgccgag aagcagagca ccggcgtctc gctcgccatc aaggcgctct tcgacggcaa
     1861 gcagtacaag gcgggcgagt ccgtcccgct ccgcttcagg ctcaccgact cgaccaccgg
     1921 ccagccgctc aagggcttga aggacgtgca ggttctcgcc ttcgagccgc cgggcatctg
     1981 gcagcagcgc cagtgggcga ccgaggtggg cgaaggcgtg tacgaagtgt cgcagacctt
     2041 cccgcgcccc ggactctaca aagtgatgct gcgctccgcg tcgcgcggcg ccgctttcgc
     2101 cgacttaccc tacacctccg tgccggtcac gagcggcgcg gagaccgtcg agaaagacaa
     2161 caccaggaat tgaatccacg aagagaggaa ccgaagaaca tgaaacgcac gattgtcacc
     2221 gccctcgcct ccgtcgccct gctcgcgttc gtctcgacgc aggccgcctc caccaacaca
     2281 cagcagaccg agcagacggc cacgccgcgc accggccaga agacgacgaa gcccgtccag
     2341 acttcttgcc ccatgcaccc cgagatcaag acgcgcagcg cgggcaagtg cccgaagtgt
     2401 cgcatggaag cgcgcaagca gagcgccgct cgcgcgaaga gcaatggcaa ggttcagccg
     2461 cagccgcagc agcaggaagg cgccgaagcg aatgattaac aggaacacag ccgcgctcgc
     2521 gctcttcgcg gcgcacttgc tcgcgcccgc cttcgcgggc ggcggcggcc acgcccacgc
     2581 gcgacagcaa aagcacgaac acaagtcgca gccggccacg cccgcgcgca agacggtgac
     2641 ccggtacgcc tgcccgatgc acccggacgt ggtctccgac gcgcccggca gttgtcccga
     2701 gtgcggcatg ttcctgaagg cggcggacgg cgagaaggcc gcgcccgcga agatctcgga
     2761 gtctgcggca gccgccgcgc ccgccaggcc cacggagcct gtcgcgcccg gcgacgcgcc
     2821 gcaaatcccg gacctgtccg tctacgacca gcagggccgc cgcctcaagt tctacaccga
     2881 cctcgtcaag gggaagaccg tcgccatcaa cttcgtcttc accacctgca ccaccatctg
     2941 cccgccgctc tcggccacct tccgcaaggt gcagcaggag ctgggcgaga gggtcgggaa
     3001 agatgtcgag ctgatttcga tcagcgttga cccggcgacc gacacgccgg agcggctgaa
     3061 gggcttcgcc gagaagttcc aggccgggcc ggggtggaca ttcgtcaccg gcggcaagcc
     3121 cgagatcgac tcgctgctca aggcgctcgg cgcggccacg cccgacaaga acgaccacac
     3181 gccgatgatc ctcgtcggca acgaggcggc gggcaagtgg acgcgcacct acggcctcgc
     3241 gcccgccgcg acgctcgtca aggtcatcgg cgaggccgcc gaagccccaa cgctctccgg
     3301 ggcttcggcg ggctacttcc cgaaccacgt cctcgtcacg caggacaaca aacccgtccg
     3361 cttctacgac gacctcgtca aaggcaaagt cgtcctcatc aacttcatgt tcaccacctg
     3421 caccggcgtc tgcccgccga tggccaccaa tatggcgaag gtgcagagct atctcggcga
     3481 ccgcatcggg cgcgacgtgg tgatcctctc gatcagcgtc gatccgaccg tcgatacgcc
     3541 cgacaagctc aagcgctacg ccgacagctt caaggcgcgg cccggctggt acttcctcac
     3601 cggcgagaag cagaacgtcg attgggtgct ttacaaactc ggcggctacg tcgaagacaa
     3661 gatgaagcac agcggcgtcc tcatcatcgg caacgagtcc accggcgagt gggtcaagac
     3721 cgccgccctg cgcaaccccg cgcagatcgc cgaggccgtc acggcgctgc tcaaaacgga
     3781 gaagaagtaa cttccgcagc agttgtcgaa agtgtgagac gttcggcaag actgtagggg
     3841 cagggcttgt ccctgcccgc cgtgacgcgg cccttcaaca ttgaagccgc cagtcgcgac
     3901 gggcagggac aagccctgcc cctacagtca tcacgtacgc gtcacgtcca acggcagaga
     3961 attatatctt ctccgccgcg cgcgcccccg ccgcttcgac gcgcgcggtt tcggcgggcg
     4021 gctctgcggc ggctgattcg cgcatccagt gttgtagctt ctccacgtcc tttgtcaccg
     4081 tgcgcgaggc ggcgaacagg acgagcgtga ggagcacgcc gagcgcgggg atgacgtaca
     4141 tcgcgttgtg cagcccctct gcgcggaacg gttcgagcgc ctgctgagtg aggctcgtca
     4201 cgcccgccgc ggtggccgcg cgcccggtga agtagtcgct gatgaggccg gtgccgtaag
     4261 ggccgagcga cgcgccgagc acgtacatcg ccagaaagta gagcgccatc gccgtgccgc
     4321 gcagggaagg ctcggtcacg tcctgtatcg tcgagtagac ggtcgagtag tagccgtaca
     4381 tcagcgcgca gcccgtcccc atcagggcca tgaagcggac ggtctcgggc ggcgcgctcc
     4441 ccagcgcgag gaagaggaac ggcaccgagc cgaggatcgc cagcgcgccc acgagcagtc
     4501 gcccgttcgt gcggtgccgc acgacggcgt cgccgagcag cccgccgagc agcaggccgg
     4561 gcgctcccga caagccgtag acggccatcg agacgaagtt ggccgagcgt atgtccatcc
     4621 cgtggtagcg catcaggtaa ggcgtgagga acgagccgat ggcgtacatg ttgaagttgt
     4681 gcagcgcgcc cgagatgatg agccagcgca tcgtcggtgt gccgagcacc agacggtacg
     4741 cagacccttc acgcctgcgc ccgccgacct tgtgcagttc ggcggcgccg cggcgcggct
     4801 cgcgtatcag caggacggcg gcggcgcaga gcaggccggg gatgcccgcc acgtagaacg
     4861 ccgagcgcca cccgtaggag cgcgcgatgg tgccgctcac ggcgaagctc aaagcgatgc
     4921 cgacgggcag ccccagcatg aagatcgaga gcgcccaagc gcgccggctc gcggggaaga
     4981 ggtcgccgat gagcgaggtg gccgcagggg cgcacgacgc ctcgcccacg ccgacgccga
     5041 ggcgcagggc gaagagctgc cagaagtttc tggcgaaccc ggaggcaacc gtcgtcaggc
     5101 tccacacgaa cacccccgcc gagagaatcc acttgcgccc ggtcttgtcc gcgaggtgcc
     5161 cgagcggcag cccaaccacc gcgtagagca gcgtgaaggc cgtgccgagc gcgcccatcg
     5221 ccccgtcgct catcccccac tccttgcgca ccgcctcgcc gaccgcgccg agtatctggc
     5281 ggtcgaagaa gttcatcgcg ttgatggcga ataagaccca cagggcgaag tgggcactcg
     5341 cacgctccgg cttcatgaag ggccttcggc tgaagaagtt tgttcgggtg ttactgaatg
     5401 cgcgggcggc gaggggaaag ccggggatga agcgcgacgt tcacgcgtcc gatgctgcat
     5461 taccgaccgt ccggtaggca ttatgctcgg ttcaagggct acggtcaatt tactttcagg
     5521 ctcgaccctc ggcactcggc acgcggacgc gcctgcggcg tgcgagcggc ggccagtcgc
     5581 tttccgtaat ccggtgcggt atcttgggcg tcaaggataa tcgaacccct atgcacgtct
     5641 ttgtcgctgc cgggaccacc ccgccatttc tgattcagac ggtcgcgctc atcgccgcgg
     5701 gcgcgctcat cgcgtacgtc tgccagcgac tcaggctcgt ccccatcgtc ggcttcctgc
     5761 tcgcaggggt gctcatcggc ccgaacgccc tcggcctcgt gggcgaccgg gagctggtgg
     5821 acgccgccgc ggaggtgggc gtcatcctgc tgctgttcac catcggtatc gagttcagcc
     5881 tcgacagact cgcgcgcatc aagaagctca tcttcggcgg cggcggcttg caggtcgggc
     5941 tggcgacgct cctgacgacg gggctgctcg ccctcttcgg cgtggactgg cgcgccgcac
     6001 tcttcacggg cttcctcatc gcgctcagct cgacggccat cgtgctcaag gtgctcgccg
     6061 acaagagcga gacgaaccac ccgcacgggc aggtcagtct cggccttctc atctttcaag
     6121 acctcgccat catcttcatg gtgctgctcg tgccgatgct gggcggcggg ggcgggtcgg
     6181 ccctcgacat cgtgtgggcg gtcgggaagg cgggggcgat catcgccgcc gtgctcgtcg
     6241 tcgcgcggcg cttcatgccg aagctgctgg aggcggtggc gcgcacctgc tcggaagaga
     6301 tttttctgct gagcgtcatc gccatctgtt tcgggacggc ttacctgacg agcctcgccg
     6361 gggtgagcct ttcgctgggc gcgttcctcg cgggcctgct cgtcagcgag agccgtttca
     6421 gcgagcacgc cttcagcgag atactgccgc ttcagatact cttcagcgcg gccttcttca
     6481 tctcggtcgg gatgctgctc gacgtggggt tcctcgtccg caacctgccg ctcgtggcgg
     6541 cggcggtggc gctcgtgctc gccgtcaagt tcctgacgac gggcgcgagc gtgctggcgc
     6601 tcggctacaa gctgccggtg gccgccgcct ctgggctggt gctggcgcag gtcggagagt
     6661 tctcgttcgt gctggagcgc gcgggccgcg acgcggggct gtcgcccgcg ggattggggg
     6721 ccgcgggttc gcagaccttc atcgcctcga cggtggtgct gatggtggcg acgccgctgc
     6781 tcatgcaggc cggctcgaag ctggcgcgga gcatcgagtc gaagcgggcc gagagggaag
     6841 gcgcgcgcgc ggaagagacg ttgccgccgg agcacttggc cgagcttgag gatcacgtcg
     6901 tcgtcgcggg ctacgggcag gcggcgcgac gcctcgtgcg cgtgctcgac ggctcgcgca
     6961 tcccttacct catcacgacg ctcagcccgg agggcgcgaa cgaggccgag gccgaggggc
     7021 tgcccgtgct gcgcggcgac gcgagcaagc ggcgcacgct cgaactggcg ggcgtcgagc
     7081 gcgcgaagat gctggtcgtg gccgacgacg agccgtcgat ggcgcgcagg gtgacggcgg
     7141 tcgcgcgggg gttgaacccg acggcgcgca tcgtcgtccg cacgcgctac ctcgccgagg
     7201 tcgagcggct gcgcgaggcg ggcgccgaca gggtcgtggc cgacgagctt gagtccatcg
     7261 tcgcgctctt cagcgacgtg ctgcgcgact accgcatccg cgccgaggag atcgatgcgc
     7321 acgaggaggc gatccgccgc ggcgactacg cggcgctgct gcaagaccgc gccgagggcg
     7381 agcagcccgc ggtcgtgtgc agccccggcg cagactgctt cgacacacgc accgcgacgg
     7441 tgcgcgaggg cgcgcccgtc acgcgcatga gcctcacgga gctttcgctc ggcgaccacg
     7501 gcgtcgaact gcgcgaggtt cgcaggaacg gcgagttgat cgaagacgtg tccgaagact
     7561 tcgtgccgtg ggcgggcgac gaactagtgc tcgtcgggca gccggacgcc ttcgcgcgca
     7621 tcgcttctct cttcagggcc gacgatggcg agccgccggt gaaacacgac gaaatcaagt
     7681 tcgaagacaa cggcacgagc cgcgcgttgg tagacacgga gcgcgttgtc acgcttgatg
     7741 tcgaaaacgc ctccgcgaac tgttcgcacc tcggcgaggt gcgtcccgtg atgccgcgcg
     7801 cgcgcggttg cgaggagtgc ctgcgaacga gcgacgagtg ggtgcatctg cgtctctgca
     7861 tgacgtgcgg gcacgtcggc tgctgtgaca cttcgaagaa caagcacgcc tcgaagcact
     7921 acccggccac cggccacccc gtcatcaagt cgctcgaacc gggcgaagac tgggcgtggt
     7981 gctaccccga cgagacttac ctttagctgc gaccgcggct tgataactgt aggggcaggg
     8041 cttgtccgcg gcccgtcgct tccgattaac tcgaagttga atcaatcgga actcgcgggc
     8101 cgcg
//
LOCUS       DBNGOH010000351         5381 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2069587, whole genome shotgun sequence.
ACCESSION   DBNGOH010000351 DBNGOH010000000
VERSION     DBNGOH010000351.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5381)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5381)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5381
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2069587"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..573
                     /locus_tag="AC159P_06905"
     CDS             <1..573
                     /locus_tag="AC159P_06905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HIR3695222.1"
                     /translation="LAGHTWKPLYDPEGILSTIPAIATTLCGVLTGHLLRSDRTPVEK
                     VAAMFVAGAACVVIGWAWNFWFPINKALWTSSFVVFMAGLALQLLALCYWRIDLKGYR
                     RWAVPFVIFGTNALALYFLAELFSHVISIVNVTRADGTRVDLKTFIFESVYAPLASPE
                     AASLMFALCTVLLWLGVMAILYRRRIFIKV"
     gene            complement(746..922)
                     /locus_tag="AC159P_06910"
     CDS             complement(746..922)
                     /locus_tag="AC159P_06910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HIR3695223.1"
                     /translation="MLDYVAQLTRDATRITPEYHERLRAVGFDDRDILQITLIASWFN
                     YINRVADALGVGRE"
     gene            complement(1031..1315)
                     /locus_tag="AC159P_06915"
     CDS             complement(1031..1315)
                     /locus_tag="AC159P_06915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HIR3695224.1"
                     /translation="MTWIKTVPAAEADDELRRALEAARTSYPIEYATPVHAVNDGEAA
                     GIVASHSLIPQALYHAFSTFGVLMSPDLKLTREQHEMITTVVSVANRCHY"
     gene            complement(1312..2685)
                     /locus_tag="AC159P_06920"
     CDS             complement(1312..2685)
                     /locus_tag="AC159P_06920"
                     /inference="COORDINATES: protein motif:HMM:NF024910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HIR3695225.1"
                     /translation="MNNVYTDDRLTRRIGLRTATALVVGETIAVGIFLTPAGMAKSIG
                     SPALVFSVWLLMGAMAVCGALCYGELAARFPEAGGGYVYLREAYGEGLAFLYGWMALV
                     VMDPGITAALAAGLGQYAGYVFGLSPAAVKAVAVASILLLAAANVYGLEFGARLMRWL
                     TALKIGLLLFVLAWGFAFRLGDWSNFSPFFAQREGSAPLVAALAGGLVAAFFSFGGWW
                     DLSKLAGEVKDPARNLPRALVRGVLVVTAVYILTTAAFIYLVPPERVTSGETFAAQAG
                     EALFGRHGGAVFSAVVIVAVAGSLAGLIMAAPRVYYAMARDGVFFRGVARVHAKFGTP
                     ARAIAVQAALASLLVLAGNFEEIIAYFIFVVVLFIALTVAGLFRLRRQPVAPRPEYLT
                     PGYPFTPAAFLSLIAVLLLLLGGNNPRQALLGVAAVAAGLPVYYIFFRRRRRHARVAA
                     AVEETLS"
     gene            complement(2943..>5381)
                     /locus_tag="AC159P_06925"
     CDS             complement(2943..>5381)
                     /locus_tag="AC159P_06925"
                     /inference="COORDINATES: protein motif:HMM:NF051698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HIR3695226.1"
                     /translation="QFGRGGSVTVDGADTNDDVVGGAVQNISQYAVQEFQVATNRFDA
                     RFGRTGSAVVNIVTKAGTNDFHGSASAFFRDRRLQGLPATFDRTSGEEPPFDRQQYAF
                     TLGGPIQENKAWFFGAVENRNQDGAVLVGTRDAARRSIINGFADAPLDDLLSNARADW
                     NPTNVDTLTLRHSFQREESVAASTLVRSIGSASQRQSSYNRTHSLLANYTRVFSPTVV
                     NVFSFSYSNFLNRTVPVTPGPQLTFPSIQDGASFRVPQQTRQQRYQFSDTLSMLRGTH
                     AIHVGGEFQHVRSGFRLDVFQQGRVELIEDFAAFDRNGDGRTDDLDLLFAVTLRSAFP
                     ERPLLIPDANNNYFAAFIQDDWRVRPNLTFNLGVRYEIDTDVKNVSRFDEINPLVRPF
                     LSGGDRGRDANNFAPRVGFHWATADGETSLHGGYGIYYDRVTLQVQSLERGLDGRALP
                     IEVRAGNVFFIEPQCLFNPACGQFPPPAPTLASPFTGFVLPGAGAGGINIIDNDLQNP
                     SVQQFNLGLQHRFAGDFVVRADYLHNFGTHFIIGRTIGQVFNPVVGGPDLVKNLESSV
                     KTKYDGLLVSVEKRFSRGFQFRSSYTLSKAFNYANDDQIPFSNGPIDSNNLQLEYGPT
                     PNDQRHRFTFAGVLDAPLGFRLSPLLTVASGVPMDILLPDGSARIPSLQRNAGGRLFR
                     TGTELNAFLAEVNAAGGTGGQPLPLVSPDARFSDSFNSLDLRVTRPFRFGERVRLEPI
                     VEVFNLFNVTNVLGVSNVNYSGFSNVLVRDSNDSASPGFLRSSSFGRPVTTAGGVFGS
                     GGPRAFQLAARLTF"
BASE COUNT          905 a         1594 c         2058 g          824 t
ORIGIN      
        1 ctggcgggcc acacgtggaa gcctctgtac gacccggagg gcatcctgag cacgatcccg
       61 gcgatagcga cgaccttgtg cggcgtgctg accgggcacc tcctgcgctc cgaccggacg
      121 ccggtcgaga aggtggcggc gatgttcgtg gcgggcgcgg cgtgtgtggt tatagggtgg
      181 gcgtggaact tctggttccc gatcaacaag gcgctgtgga cgagttcctt cgtggtcttc
      241 atggccgggc tggcgctgca actgctcgcg ctctgctact ggcggatcga cctcaaaggt
      301 tatcggcggt gggccgtgcc gttcgtcatc ttcggcacga acgcgctcgc gctctacttc
      361 ctcgcggagc tgttctcgca cgtcatctcc atcgtcaacg tcacgcgcgc cgacggcacc
      421 cgcgtggact tgaagacctt catcttcgaa agcgtctacg ccccgctggc ctcgcccgaa
      481 gccgcctcgc tcatgttcgc cctctgcacc gtgctgctct ggctcggcgt gatggcgatc
      541 ctttaccgga ggcggatctt catcaaggtg tgaaaggcag taggcggtag gcggtaggca
      601 gcaggcagta aagacaaaag cgggaagagg cttgagaggt aaaatcccaa gcctcctcga
      661 tcttcaagcg ataatgcgag aggcttgaga caatgaatct caagcctctc gctttgactg
      721 cctaccgcct accgcctacc gcctgctact cccgccccac cccgagcgcg tcggcgacgc
      781 ggttgatgta gttgaaccag gaggcgatga gcgtgatctg gaggatgtcg cggtcgtcga
      841 agccgacggc gcgcaggcgc tcgtggtatt cgggcgtgat gcgcgtggcg tcgcgggtga
      901 gctgcgcgac gtagtcgagc atgacgcggt tctgctcgga gatgggcgcg gtgcggtagt
      961 cctcgcggat ggccgcgacg agttcttcgt tgagcgtgac gcgacgcaga aactctgcgt
     1021 gggactcgat tcaatagtgg cagcggttgg cgacggagac gaccgtggta atcatctcgt
     1081 gctgctcgcg cgtgagcttg aggtcgggcg acatgagcac gccgaaggtc gagaaggcgt
     1141 ggtagagcgc ctgcggaatg aggctgtgcg aggcgacgat gccggcggcc tcgccgtcgt
     1201 tgacggcgtg gacgggcgtg gcgtactcga tggggtagct cgtgcgcgcg gcttcgagcg
     1261 cgcggcgcag ctcgtcgtcg gcctcggcgg cggggacggt cttgatccag gtcatgaaag
     1321 cgtttcctcc actgcggcgg cgacgcgcgc gtgacggcgg cggcgccgga agaaaatata
     1381 gtagacgggc agccccgccg ccaccgccgc gacgccgagg agcgcctgcc gcgggttgtt
     1441 gccgccgagc agcaggagca gcacggcgat gagcgagagg aaggcggcgg gcgtgaacgg
     1501 ataaccgggc gtcaggtatt cggggcgcgg cgcgacgggc tggcggcgca gacggaagag
     1561 gccggcgacc gtgagcgcga tgaagaggac gacgacgaag atgaagtagg cgatgatctc
     1621 ttcgaagttg cccgcgagga cgagcaggga ggcgagcgcg gcctgcacgg cgatggcgcg
     1681 cgcaggcgtg ccgaacttcg cgtggacgcg cgcgacgccg cggaagaaga cgccgtcgcg
     1741 cgccatcgcg tagtagacgc gcggggccgc catgatgagt ccggcgaggc tgcccgcgac
     1801 cgccacgatg acgaccgcgg agaagaccgc gccgccgtgg cgaccgaaga gcgcctcccc
     1861 ggcctgcgcg gcgaaggtct cgcccgaggt gacgcgctcg ggcggcacga ggtagatgaa
     1921 ggcggcggtg gtcagaatgt agacggcggt cacgaccagc acgccgcgga cgagcgcgcg
     1981 cggcaggttg cgcgcggggt ctttgacttc gcccgcgagc ttcgacaagt cccaccagcc
     2041 gccgaacgag aagaaggccg cgaccagtcc tccggcgagt gccgcgacga gcggcgcgga
     2101 gccttcgcgc tgcgcgaaga agggcgagaa gttcgaccag tcgccgagcc ggaaggcgaa
     2161 gccccacgcg agcacgaaga gcaggaggcc gatcttgagc gcggtgagcc agcgcatcag
     2221 ccgcgcgccg aattcgaggc cgtagacgtt cgccgccgcg agcagcagga tcgaggcgac
     2281 ggcgaccgcc ttcaccgcgg cgggcgagag gccgaagacg tagcccgcgt actggccgag
     2341 gcccgcggcg agcgcggcgg tgataccggg gtccatcacg acgagcgcca tccagccgta
     2401 gaggaaggcc agcccctcgc cgtaggcttc gcgcaggtag acgtagccgc cgcccgcttc
     2461 cggaaagcgc gccgccagct cgccgtagca gagcgcgccg cacacggcca tcgcgcccat
     2521 caacagccac acggagaaga cgagcgcggg cgagccgatg ctcttcgcca tcccggcggg
     2581 cgtcaggaag atgccgaccg cgatggtctc gccgacgacg agcgccgtcg ccgtgcgtag
     2641 gccgatgcgt ctcgtcagcc ggtcgtcggt gtacacattg ttcatcgaac gattcgaatg
     2701 gagtgcgcgt cacgtcgccg cgtagcggcg gttgaatgta gcccggcctt tcaaggccgg
     2761 gaatagcatc ggccatccgt gggcgtcggg tagcgacgcg tgaatcttgc ggcgcccgtt
     2821 caggcgtcgc tgacgcgacg cggcgtcgtt gttcgtatgg gtctgacccg gtcttgaaag
     2881 gccgggctac attcaaccgc cgctacgcgg cagagttaca acgctacgcg ttcacggcag
     2941 ggctagaacg tcaggcgcgc ggcgagctgg aaggcgcgcg ggccgccgga gccgaagacg
     3001 ccgccggcgg tggtgaccgg cctgccgaag ctcgacgagc ggaggaagcc gggagaggcc
     3061 gagtcgttgc tgtcgcgcac cagcacgttg gagaagccgg agtagttgac gttcgagacg
     3121 ccgagcacgt tcgtcacgtt gaagaggttg aagacctcga cgatgggttc gagccggacg
     3181 cgctcgccga agcggaaggg gcgcgtgacg cgcaggtcga gcgagttgaa gctgtcgctg
     3241 aagcgcgcgt ccggcgagac gagcgggagc ggctggccgc ccgtcccgcc cgccgcgttg
     3301 acctcggcga ggaaggcgtt cagctccgtg ccggtgcgga aaaggcgccc gcccgcgttg
     3361 cgctgcaaag aggggatgcg cgccgagccg tcgggtagga ggatgtccat cggcacgccc
     3421 gaggccacgg tcagcagcgg cgagaggcgg aagccgagcg gcgcgtcgag cacgcccgcg
     3481 aaggtaaagc ggtggcgctg gtcgttgggc gtggggccgt attcgagttg caggttgttc
     3541 gaatcaatgg ggccgttgga gaagggaatc tggtcgtcgt tggcgtagtt gaaagccttc
     3601 gagagcgtgt aggaggagcg gaactggaag ccgcgcgaga agcgcttctc gacgctcacg
     3661 aggaggccgt cgtacttcgt cttgacgctc gattcgaggt tcttgacgag gtcggggccg
     3721 ccgaccacgg ggttgaagac ctggccgatg gtgcgcccga tgatgaagtg cgtgccgaag
     3781 ttgtggaggt agtcggcgcg cacgacgaag tcgcccgcga agcggtgctg tagcccgagg
     3841 ttgaactgct gcaccgacgg gttctggagg tcgttgtcga tgatgttgat gccgcccgcg
     3901 cccgcgcccg gcaggacgaa gccggtgaag ggcgaagcga gcgtcggcgc cgggggcggg
     3961 aactggccgc aggccgggtt gaagaggcac tgcggctcga tgaagaagac gttgccggcg
     4021 cgcacctcga tgggcagcgc gcgaccgtcg aggccgcgtt cgagcgactg cacctgaagc
     4081 gtgacgcggt cgtaatagat gccgtagccg ccgtggagcg aagtctcgcc gtcggcggtc
     4141 gcccagtgga agccgacacg cggggcgaag ttgttagcgt cgcggcctcg gtcgccgccg
     4201 ctgaggaagg ggcggacgag cgggttgatc tcgtcgaagc ggctgacgtt cttcacgtcc
     4261 gtgtcgattt cgtagcgaac gccgaggttg aaggtgaggt tggggcgcac gcgccagtcg
     4321 tcctggatga aggcggcgaa gtagttgttg ttggcgtcgg ggatcaggag cgggcgttcg
     4381 gggaaggcgc tgcggagcgt gacggcgaag agcaggtcga ggtcgtcggt gcgcccgtcg
     4441 ccgttgcggt cgaaggcggc gaagtcttcg atcagctcga cgcgcccctg ctggaacacg
     4501 tcgaggcgaa agccggagcg gacgtgctgg aactcgccgc cgacgtggat ggcgtgcgtg
     4561 ccgcgcaaca tcgagagcgt gtcggagaac tggtagcgct gctggcgcgt ctgctgcggc
     4621 acgcggaacg aggcgccgtc ctgaatcgag gggaacgtga gctgcggccc cggagtgaca
     4681 gggacggtgc ggttgaggaa gttgctgtag ctgaagctga agacgttgac cacggtgggc
     4741 gagaagacgc gcgtgtagtt ggcgagcagc gagtgcgtgc ggttgtagct cgactggcgc
     4801 tgcgaggccg agccgatgga gcggacgagc gtgctcgcgg cgacggactc ttcgcgctgg
     4861 aacgagtggc gcagcgtgag cgtgtcaacg ttggtcggat tccagtcggc gcgcgcgttg
     4921 ctgaggaggt cgtcgagcgg ggcgtcggcg aagccgttga tgatcgagcg ccgcgccgcg
     4981 tcgcgcgtgc cgacgaggac ggcgccgtcc tgattgcggt tctcgaccgc gccgaagaac
     5041 cacgccttgt tttcctggat ggggccgccg agcgtgaagg cgtactgctg gcggtcgaag
     5101 ggcggctcct cgccgctcgt gcggtcgaag gtcgcgggca gaccttgcag gcggcggtcg
     5161 cggaagaagg ccgaggccga gccgtggaag tcgttcgtgc cggccttcgt gacgatgttg
     5221 acgaccgccg agccggtgcg cccgaagcgc gcgtcgaagc ggttggtggc gacctgaaac
     5281 tcctgcacgg cgtactgcga gatgttctgc accgcgccgc cgaccacatc gtcgttcgtg
     5341 tcggcgccgt ccaccgtgac gctgccgccg cgcccgaact g
//
LOCUS       DBNGOH010000352         3146 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3536211, whole genome shotgun sequence.
ACCESSION   DBNGOH010000352 DBNGOH010000000
VERSION     DBNGOH010000352.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3146)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3146)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3146
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3536211"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(135..1277)
                     /locus_tag="AC159P_06930"
     CDS             complement(135..1277)
                     /locus_tag="AC159P_06930"
                     /inference="COORDINATES: protein motif:HMM:NF014523.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HIR3695227.1"
                     /translation="MPPKKSTGLRELRVGLLVVVSIGVLIFLILNASGDISPFTKKVK
                     LRARFANADGLRRGSEVRLAGVRIGKVDDVNLLGPSEKANEKVEALFSIDDKIDGRPA
                     NDIIRNDSTARLGSPSLLGTDKLINLTPGTSVGTPVKDGDLLPPGQAQGDLDALTASG
                     NELMQQLNKLSVQFTDIARKVNEGQGTFGRFVNDEAFYNSLNSTIRDTNEVIRLIRSG
                     DGSAGRFVNDPALYNNLNSVSLSLQQIAEDLRAGRGTAGKLLTDDAVYNEARATIARL
                     NRSVDEVNFIVADLRAGRGTAGKLLTDETIYNDARAAIARFNTAAERIDNVVAGVQRG
                     EGTAGKLLTDDQLYSNVNQLSAESVKLIYDFRQNPKKYLTIKFELF"
     gene            complement(1433..2344)
                     /locus_tag="AC159P_06935"
     CDS             complement(1433..2344)
                     /locus_tag="AC159P_06935"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3695228.1"
                     /translation="MASTDVEVHRTDPGMVGGVSEDAAESTFAEVDDRARIIPAVEFR
                     DVSLSFDDRKILDGLSFKVQKGESKIILGGSGGGKSTTIKLILGLLKPDAGRILVDGE
                     DITEYTEAEMMKVRKKIGMIFQEGALFDSLSVYDNVAYRLHEQGVPEEEVEQEVRRML
                     QFVNLEDAIDKMPIELSGGMRRRVGIARALVGGPKIVMFDEPTAGLDPPTARTICELA
                     IKLRDLEDVSSMFVTHEMNNVAYLTQEYAVVNDAGDVVFEREGERLCLVNTKVLMLRN
                     GKIIFEGTDEALHRTEDPYIQRFIRGH"
     gene            complement(2363..>3146)
                     /locus_tag="AC159P_06940"
     CDS             complement(2363..>3146)
                     /locus_tag="AC159P_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013568392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MlaE family ABC transporter permease"
                     /protein_id="HIR3695229.1"
                     /translation="TENVRMNIFLRALLEVQETTLLAARAFGRLFRRPAYLPEMITQM
                     DVIGVGSLTIILLTGFFTGGVLALQTYPTLKYYGAQDQTGRLVATALVRELGPVLSGL
                     MVAGRVASAIAAELGSMVVSQQIDAMRALGTDPVRKLVAPRLIALLVTLPLLTLISDA
                     VGIFGGWVVGTQLYDMASGTFTAAVWEGLTTDDISGGFIKAACFALIVGTVACRQGLR
                     TEGGTVGVGRSTTTSVVMSSILIIVADFFITKGLQVILGVPS"
BASE COUNT          504 a          925 c         1128 g          589 t
ORIGIN      
        1 ccagccggtc ggacgcgtct cgctcgacgg cttcagcccg cgccccggtg tcagcgtctg
       61 cggtgtcccg ctgtggggct gaccgagtcg gcccccgcac cgggcgcgca atttggccgg
      121 caacagcaat cgggtcagaa tagttcgaat ttgatggtga gatacttttt ggggttttgg
      181 cggaagtcgt agatgagttt gacggattcg gcggagagtt ggttgacgtt ggagtagagc
      241 tggtcgtcgg tgaggagttt gccggcggtg ccttcgccgc gttggacgcc ggcgacgacg
      301 ttgtcgatgc gttcggcggc ggtattgaag cgggcgatgg cggcgcgggc gtcgttgtag
      361 atggtctcgt ccgttaagag tttgccggcg gtgccgcgac ccgcgcgcag gtcggcgacg
      421 atgaagttga cttcgtcgac ggagcggttg aggcgggcga ttgttgcgcg cgcctcgttg
      481 tagacggcgt cgtcggtgag gagcttgccg gcggtgccgc gcccggcgcg gaggtcttcg
      541 gcgatctgct ggagcgagag gctgacggag ttgaggttgt tgtagagcgc ggggtcgttg
      601 acgaaccgac cggcggaacc atcgccggag cggatgaggc ggatgacttc gttggtgtcg
      661 cggatggtcg agttgaggct gttgtagaag gcttcgtcgt tgacgaagcg accgaaggtg
      721 ccctggccct cgttgacctt acgggcgatg tcggtgaact ggacggagag cttgttgagc
      781 tgctgcatca gttcgttgcc ggaggccgtg agcgcgtcga ggtcgccttg cgcctggccg
      841 ggggggagca ggtcgccgtc cttgacgggg gtgccgacgg aggtgccggg cgtgaggttg
      901 atgagcttgt cggtgccgag cagggagggc gagccgaggc gcgcggtcga gtcgttgcgg
      961 atgatgtcgt tggcgggcct gccgtcgatc ttgtcgtcga tggagaagag cgcctcgacc
     1021 ttctcgttcg ccttctcgga cgggccgagc aggttcacgt cgtccacctt gccgatgcgc
     1081 accccggcga gccgcacctc cgagccgcgc cggaggccgt cggcgttggc gaagcgcgcg
     1141 cgcagcttga ccttcttcgt gaagggcgag atgtcgcccg acgcgttgag gatcaggaag
     1201 atcagcacgc cgatggagac gacgaccaac aggccgacgc gcagctcgcg caacccggtg
     1261 ctcttctttg gaggcatggc atcgaagtga tgaggactta ggactgaaga ctgagtaaag
     1321 acaagtgacg ggcgatgatt gatgagaggt atgctgtgcg gactcgtcgg tcgtcacagg
     1381 tctctcgctt ttactcagtc ctcagtcctc agtcctcatc actctccttt cttcagtgtc
     1441 cgcggatgaa ccgctggatg tacgggtcct cggttcggtg gagggcttcg tcggtgccct
     1501 cgaagatgat cttgccgttg cggagcatca ggactttggt gttgacgagg cagaggcgct
     1561 cgccctcgcg ctcgaagacc acgtcgcccg cgtcgttgac gacggcgtac tcctgcgtga
     1621 gatacgcgac gttgttcatc tcgtgcgtga cgaacatcga ggagacgtct tcgaggtcgc
     1681 ggagcttgat cgccagctcg cagatggtgc gcgccgtcgg cgggtcgagg ccggcggtcg
     1741 gctcgtcgaa catgacgatc ttggggccgc cgacgagcgc gcgcgcgatg ccgacccggc
     1801 ggcgcatgcc gcccgacagc tcgatgggca tcttgtcgat ggcgtcttcg aggttgacga
     1861 actggagcat ccgcctgacc tcctgctcga cctcttcttc gggcacgccc tgctcgtgca
     1921 gacggtaggc gacgttgtcg tagaccgaga gcgagtcgaa gagcgcgccc tcctgaaaga
     1981 tcatcccgat cttcttgcgc accttcatca tctcggcctc ggtgtactcg gtgatgtcct
     2041 cgccgtccac gaggatgcgg cccgcgtcgg gcttcaagag gccgaggatg agcttgatgg
     2101 tggtggactt gccgccgcca gagccgccga ggatgatctt cgactcgccc ttctgcacct
     2161 tgaagctgag gccgtcgaga atcttgcggt cgtcgaagga gagggacacg tcgcggaact
     2221 cgaccgccgg gatgatgcgg gcgcggtcgt ccacctcggc gaaggtggac tccgccgcgt
     2281 cctcgctgac gccgccgacc atgccggggt ctgtgcggtg aacttcaacg tcggtggaag
     2341 ccatgtgaat cgtccgcgtc ccttacgaag gcacacctag aataacctga agccccttcg
     2401 ttataaagaa gtcggcgacg attatcaaaa tcgaggacat cacgaccgag gtcgtcgtcg
     2461 agcgcccgac gccgaccgtg ccgccctcgg tgcgcaaccc ctgccggcag gcgaccgtcc
     2521 cgacgatgag cgcgaagcag gccgccttga tgaagccgcc ggagatgtcg tcggtcgtca
     2581 gcccttccca caccgccgcc gtgaaggtgc cggaggccat gtcgtagagc tgcgtgccga
     2641 ccacccaccc gccgaagatg ccgacggcgt cggagatcag ggtgagcagc ggcagcgtga
     2701 ccagcagcgc gatgaggcgc ggcgcgacga gcttgcgcac cgggtccgtg ccgagcgcgc
     2761 gcatggcgtc gatctgctgc gagacgacca tcgagccgag ttccgccgcg atggccgagg
     2821 cgacgcgccc cgccaccatc agcccgctca agaccggccc cagctcgcgc acgagcgccg
     2881 tcgccacgag cctgccggtc tgatcctgcg cgccgtagta cttgagcgtc gggtaggttt
     2941 gcagcgcgag gacgccgccc gtgaagaagc ccgtgagcag gatgatggtc agcgagccga
     3001 cgccgatcac gtccatttgc gtgatcattt cggggaggta agcggggcgg cggaagagcc
     3061 tgccgaaggc gcgggccgcg agcagggtcg tctcctgtac ttcgagcagg gcgcgcagga
     3121 aaatgttcat ccggacgttc tcggtg
//
LOCUS       DBNGOH010000353         3660 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1887583, whole genome shotgun sequence.
ACCESSION   DBNGOH010000353 DBNGOH010000000
VERSION     DBNGOH010000353.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3660)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3660)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3660
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1887583"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..742
                     /locus_tag="AC159P_06945"
     CDS             <1..742
                     /locus_tag="AC159P_06945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695230.1"
                     /translation="LPMSPSRILPSNAERTGARRRLGPYLVAVPVTAITFSLASLALR
                     LGAPGIVGGAVVGFVWSLSLGSVARRLGRRGSWRPRLADAPVFLAVIASGLLIGGGLM
                     YGLLIEAALREPSTTNATLSALMRPAVPYFIAVNTLMEVLVIPLAVYWNWHVPKRRTL
                     VVIAVTAYFAMRVWSYLTYAPARLDISTRTLSAAEAEWFKETLKADYRGVLNVITHVA
                     FILAAFVPPRAAEGRAAGAPGISRDRAA"
     gene            861..1772
                     /locus_tag="AC159P_06950"
     CDS             861..1772
                     /locus_tag="AC159P_06950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695231.1"
                     /translation="MKTALPVVLKVLALTLILFACYAIAGGVVGPRGDSPTPEEAGGA
                     APALLAACLLITLVLTHLILRSRWGGGRLMAAVFFVFYGVTTFMSQIESAVFVTRLPP
                     GTLPRLFLMGALVAAPFSVLAVLILGKRRADAGDAEGDARLVMPAGEWAWKLAAIALT
                     YLILYFTFGYFIAWRDPAVREYYGGIDEGSFFAHMEALVRGDTSWLIPFQILRAMLWV
                     ALALPVIRMMKGSWPETALAVGLLFSVVMNAQLLLPNPYMPEGVRMTHLIETASSNLI
                     FGLVVGWLLARRHEFGQVPAPQNKGAA"
     gene            1889..2380
                     /locus_tag="AC159P_06955"
     CDS             1889..2380
                     /locus_tag="AC159P_06955"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011422853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase family protein"
                     /protein_id="HIR3695232.1"
                     /translation="MLLFLKNLLFTAIVPGTVAVYVPLSLAPPPTELTFGWGIMQVVA
                     LLPLVAGCAVYLWCLWDFAAFGRGTPAPIDAPKRLVVRGLYRYVRNPMYVGVLLAVAG
                     WALLFQSLRILGYGAVVALCFHLFVVAVEEPSLRGKFGDSYERYCREVRRWVPGVSRG
                     RAA"
     gene            2492..3124
                     /locus_tag="AC159P_06960"
     CDS             2492..3124
                     /locus_tag="AC159P_06960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695233.1"
                     /translation="MAETSLPKWTGMAGVATFALILATVPFYFVYSGPPPARNVLARS
                     LVGMFSLVALLGFVVGLQALARRARPGEEAPATLLGSLGVILVAVDFVAIAQEAGAVL
                     RRTDPFDPTLVGSGAEGALVIYGPVGRILTAALLAAAGAVTVRARLLPAGVGWAAFAL
                     GAVQLAFVATYFSGTDPAQFFSVNGWHLPVLGSLLGLWILSASVALLRRN"
BASE COUNT          483 a         1222 c         1250 g          705 t
ORIGIN      
        1 gcttccgatg agcccgtccc gaattcttcc ctcgaacgcc gaaaggactg gagcccggag
       61 acggctcgga ccctacctcg tggccgtgcc cgtcaccgcg atcactttct cgctcgcctc
      121 cctcgccctt cggctcggcg cgccgggcat cgtcggggga gcggtcgtgg gattcgtgtg
      181 gagcctgtcc ctcgggtccg tcgcgaggcg gctcggccgg agggggagtt ggaggccgcg
      241 tctggccgac gcgccggtct tcctggccgt cattgcgtcg gggttactca tcgggggcgg
      301 gctgatgtac gggctgctca ttgaggcggc gctgcgcgag ccttcgacca ccaacgcgac
      361 cctgtccgcc ctgatgcggc cggccgtccc gtacttcatc gccgtcaaca cgctgatgga
      421 ggtgctcgtc atcccgctgg ccgtctactg gaactggcac gtcccgaagc gtaggacact
      481 cgtcgtcatc gccgtcacgg cgtacttcgc gatgcgcgtg tggagctacc tgacctacgc
      541 gccggcgagg ctcgacatct ccacccgcac gctctcggcc gcggaggcgg agtggtttaa
      601 ggagacgctc aaggctgact acaggggcgt gctgaacgtc atcacgcacg tggccttcat
      661 cctggcggcg ttcgtcccgc cccgggcggc cgagggccgg gcggcaggcg cgccggggat
      721 aagccgcgac cgagccgcct aacgacggcc cgcacccgac ccgcgacacg gcggattcca
      781 ctattcagca tagggctcgg gcgggcgggc gacgcgaggg ttagcttgtg tcgtgcggcg
      841 gccggccccg gaagaagaat atgaaaactg ctctcccagt cgttctaaaa gtactggccc
      901 tgacactgat tctgttcgcc tgctacgcca tcgcgggtgg ggtcgtcggg ccgcgaggcg
      961 attctccgac gcccgaagaa gccggcgggg cggcgccggc tttgctggcg gcctgcttat
     1021 tgatcacgct cgtcctgacg catctcatcc ttcgctcccg ttggggcggg gggcgcctta
     1081 tggccgccgt ctttttcgtc ttctacgggg tgacgacctt catgtcgcaa atcgagtcgg
     1141 cggtcttcgt gacgcgcctg cccccgggca cgttgccgcg cctattcctg atgggcgccc
     1201 tcgtcgccgc ccccttctca gtgctggctg ttctaatcct ggggaagaga cgagccgacg
     1261 ccggggacgc cgaaggtgac gcgcgcctgg tcatgcccgc cggggagtgg gcgtggaaac
     1321 tcgcggccat cgccctcacc tatttgatcc tgtatttcac gttcggctat ttcatcgcct
     1381 ggcgcgaccc cgcggtgcgt gagtattacg gcggcattga tgaaggcagt ttctttgccc
     1441 acatggaggc gttagtgcgg ggggatacgt cttggctcat ccccttccag attctgcggg
     1501 cgatgctttg ggtggcgctg gccctgcccg tcattcggat gatgaagggg tcatggccgg
     1561 agaccgccct ggcggtcggg ttgctttttt ccgtcgtgat gaacgcccag cttctgctcc
     1621 ctaacccgta tatgcctgaa ggggtgcgga tgacgcacct gatcgagacg gcctcgtcca
     1681 atctcatttt cggcctggtg gtcggctggc tcctcgcgcg acgccacgag ttcggtcaag
     1741 tcccggcccc tcagaacaag ggcgcggcct gacaacggcc tgcacccggc cgcgaaatag
     1801 cgcggtcttc atacgcaaga cgcccttcta ccagcgttga atgcgcggcg ggtgatgccg
     1861 ggcgtcaggc ggccagagtg agggagatgt gcttctcttc ctgaagaatt tgctgttcac
     1921 tgcgattgtc ccgggcacgg tcgccgtcta cgtcccactc agcctcgcgc cgccccccac
     1981 agagttgacg ttcgggtggg gcatcatgca ggttgtcgcg ctgctgccgc tcgtcgccgg
     2041 gtgcgccgtc tacctctggt gcctgtggga cttcgccgcg ttcggccggg gcacgcccgc
     2101 cccgatcgac gcccctaagc gcctggtcgt gcgcggactc tatcgttacg tgcgaaaccc
     2161 gatgtacgtc ggggtgctgc tggcggtcgc gggctgggct ttgttattcc agtctttgcg
     2221 catcctcggc tacggggctg tcgtcgcctt gtgcttccac ctgttcgtcg tggcggtgga
     2281 ggaaccgtct ttacgcggga agttcgggga ctcatacgag cggtattgta gggaggtcag
     2341 gcggtgggtg ccgggggtca gccgggggcg ggccgcctaa cgaagacacg cactcgaccc
     2401 ggctcggcgc gggtgtcatc cggcaacttg agggaattat acgtcgcgcg cgggcgggtg
     2461 acgcttggcg ttcggcggcg gaggcacgtt catggccgag acatcgctgc ccaagtggac
     2521 cggtatggcc ggcgtcgcca cgttcgccct catcctggcg accgtcccgt tctacttcgt
     2581 ctacagcggg ccccctcccg cccggaacgt cctcgcccgc tccctggtcg ggatgttctc
     2641 gctggtcgcg ctcctcggct tcgtcgtcgg gctccaggcg ctcgcccgcc gcgcccggcc
     2701 gggggaggaa gcgccggcca cgctgctcgg gtccttgggc gtcatcctgg tggccgtgga
     2761 cttcgtcgcg atcgcgcagg aggcgggggc ggtgctccgc cggaccgacc cgttcgaccc
     2821 gacgctcgtg ggttcgggcg cggaaggggc gctggtgatt tacgggccgg tggggcgcat
     2881 cctgacggcg gcgttgctcg ccgcggcggg ggcggtcacc gttcgcgccc gcctcctgcc
     2941 cgccggggtg gggtgggcgg cgttcgcgct cggcgcggta caactcgcgt tcgtggccac
     3001 gtacttttcc gggacggacc cggcacagtt cttcagcgtc aacggctggc acctcccggt
     3061 cctcggcagc cttctgggcc tctggatcct ctcggcgagc gtggcccttc tccgccggaa
     3121 ctgacgggga agggccattg ggtgtttagg acggccgaag ctgctgttgc ccgaggtagc
     3181 cgccgaatta ggcgctgcag cagacggccg ccgccgtgtc ggctgtctcc ttcactgaag
     3241 tagagaaggc ctccgggtag gaggcgagct tcaagggcat atattcccga ggacgtccta
     3301 ctacgggcct ggtagagcct gatcttcacg gacgcgcggc gcgggtcgag tttgaagccg
     3361 ggcggcacgg aaagtgatag cctgagtctc ccctgttcca gggccgccga aaactcctcg
     3421 accggggtgt ccccgtcacc accgcccgcc agacggatcc tgcgccgcgg ccttagcagt
     3481 cggccgttga tgcgcaggtg ccacgccgcc gtgagcagtg tggacaccct ccggcctctc
     3541 ttctccaccc agccgaccgg catcttctcc accaccagcc acacggtgtc gtagggcgcg
     3601 gcccgcatgg cctcgaaggg ccggacgaac tcgacgctgc tctcgaccgg ccactcgaac
//
LOCUS       DBNGOH010000354         5935 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2253091, whole genome shotgun sequence.
ACCESSION   DBNGOH010000354 DBNGOH010000000
VERSION     DBNGOH010000354.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5935)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5935)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5935
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2253091"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(192..809)
                     /locus_tag="AC159P_06965"
     CDS             complement(192..809)
                     /locus_tag="AC159P_06965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695234.1"
                     /translation="MKLTTPHIAFARLADLAEGRLTPEERARDEEHLAACARCSRQLA
                     ELRHVTTLMRTDDSMDVPHGLVSDAIRMFRSHPSVERAPGLLRRVLATLSFDSSALTP
                     AFGVRSGQAAPARQMLFSAGGFDIDLRLAEGGAGWTISGQVLGPCDGGGRVEVLSADD
                     QERVAAADLNDLCEFTLPPVAAGAYVLRLRLGETEVEVPGLDLGA"
     gene            complement(998..1609)
                     /locus_tag="AC159P_06970"
     CDS             complement(998..1609)
                     /locus_tag="AC159P_06970"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR3695235.1"
                     /translation="MLAGMQTGADATRQTDEQLVAACRRGDEVAWEALVKRYQRLVYA
                     VPRRAGLDEDAASEVFQEVFTTLLQSIDTIEQPSRLHAWLVTTARRKTWRVIRRAKSM
                     RPFDDERDEGEDEMLSLPDAGPLPDEALARLEQQHLVRAALAEMDERCRKLLTMLFYQ
                     PEQPSYTDIAAALGTPEGSIGPTRARCLKKLLAGLKKKGFEKP"
     gene            1693..2460
                     /locus_tag="AC159P_06975"
     CDS             1693..2460
                     /locus_tag="AC159P_06975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695236.1"
                     /translation="MFIRRFVITQVVVWCVAAGGAAGQERAGAAATGGAEQREVAAQS
                     SAEEGRLVSGSRAAIIAAGLSPEFFDAHFRLEKVFAKPADRRVVWRLKVGEYETTVND
                     SVGSYTDEKGERHDAHSITGRLSPARDIGPTIPQKRAERLMKACIGEYQSGAVVYQPF
                     GSPPRAALVFTAMSLPEPQKPAAKGSQTPPAPTGAAATTAPTLAPTQADTIRQGGKKP
                     PPLRIGMVDLETGRCVVGVGQVGSPHPSAEKYTRPRR"
     gene            2955..>5935
                     /locus_tag="AC159P_06980"
     CDS             2955..>5935
                     /locus_tag="AC159P_06980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005882 - intermediate filament [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HIR3695237.1"
                     /translation="MKRFVTPASVMTLALTLACQSLLLARAPSAVAQKRTDSKSVGVE
                     LRDADAPTGAAAQAAHRQKSAPVTAAPAPQSKQAGDAARPTSVRVTGVRTVRVNFKSL
                     VALDARKAAVRSKQPAFQQEIHSPGTIPEVGSEGASQASAEDDATLAPAAEPQDTGGP
                     LVPSPAPAQNFLAQEDGAKVGTGTFTIPPDTMGAVGFDKVFVNVNNNYRVQNKATGAA
                     LSTVSIDTFWSSTGATGVFDPRIQFDPYQNRWLLSAVANAQTANSSVLVGVSDTSDPQ
                     GTYTLFRIIVGCAPGGAGCNAQGEWADFPMLGFNKNWLAVGWNQFTINTSAFVGGKML
                     VLDYPTLRTGTATGTLFTNASAATGFCMHPVTTFSATEDTLYVPTHQQSAGALYRLHK
                     ITGTPGAPTFTLDATARVRTGGGWTQPGGDNLPQQCVPGVGAPTQTCPATPRGLEASD
                     SFIRSNAVFRNGLIYYPQAIALPAGGLTVASRFAAQWTALNPDGTFNTGGRVEDAAAQ
                     IFNGGKHYSYPSLSVNKNNDILLGFSEFESDDYVDAGYTFRLGTDAAGTMRDPVIYKE
                     GEDYYQKTFSGTRNRWGDYSHTVVDPVNDRDLWTVQEYAQLRVGTTGQGSNDSRWGTW
                     WAKITAPAGAGDLLISEFRVRGPNPNGAGNEFIEIYNPNSSALTVTTLDGAAGYAVAA
                     SDGVARCVIPSGTIIPARAHYLCVNSVGYSLASHPAGNGTTATGDATYTTDIPDNAGL
                     AIFNTSVAANFALATRLDAVGSTSEANTLYKEGTGYPALTPFSIDYSFYRDNCGKGGS
                     ITTLGVCPTGGFPKDTNNNAADFIFVDTNGTSAGAGQRLGSPGPENLSSPVQRNSSFS
                     VPLLDTSVGGAIPPNRVRDFTSDPANNSTFGTLTIRRRVVNNTGAPVTRLRFRVVDQT
                     TFPAPSGFADLRTRTSTLVVVSGVNDPTTCLASNGVATTPCTINVQGTTLEQPPSQLN
                     GGAFNGTYSAGTITLGTPLAPG"
BASE COUNT          971 a         2130 c         1950 g          884 t
ORIGIN      
        1 ccaaagccac gtcggcgagc gccgtgtgct ctcgcaggag cgcgacccgc tcggcctcgc
       61 ccccggcttc gaccagccgc gctgccagtt ccgttcggtt catcattcga aagtactgag
      121 gactgagtga aagcgttgtc ttcttactca ggcctcagtc ctcagtcctc agtactttgg
      181 ttttggacgc gctaggctcc caaatcgaga ccgggaactt cgacctccgt ctcgccgagt
      241 cgcaggcgga gcacgtaggc gcccgccgcg acgggcggca gcgtgaactc gcagaggtcg
      301 ttgaggtcgg cggcggcgac ccgctcttgg tcgtcggcgc tcagcacttc gacgcgcccg
      361 ccgccgtcgc aggggcctaa gacctggccc gagatcgtcc agccggcgcc gccttcggcg
      421 agccggaggt cgatgtcgaa gccgcccgcg ctgaagagca tctggcgcgc gggcgcggcc
      481 tgtccggagc ggacgccgaa ggcgggcgtg agcgcgctgc tgtcgaagct gagcgtggcg
      541 agcacccggc gcagcagtcc gggcgcacgc tcaaccgagg ggtgagagcg gaacatgcgt
      601 atcgcgtcgg agacgagacc gtgcggcacg tccatcgagt cgtccgtgcg catcagcgtc
      661 gtgacgtgac gcagctccgc aagttggcgc gagcagcgcg cgcaagccgc gagatgctcc
      721 tcgtcacgcg cgcgctcttc gggcgtgagg cggccttcgg cgaggtcggc cagccgcgcg
      781 aaggcgatgt gtggtgtggt cagtttcatc tttccggctc cagacgggca tccttcaggg
      841 acgctcactc gcccgaacag tgcgcggtcg gcggggggaa atacataaaa aaaagccgtg
      901 aaccgtggac cgtgataagt gaaaagcggg aatggtagac cgtgaacgtt gatgagagaa
      961 gtgctgttcg aacccgtcac ggtttacggt tcacggttca cggcttttcg aatcctttct
     1021 tcttcaggcc ggcgagcagt ttcttgaggc agcgggcgcg ggtcggcccg atgctgcctt
     1081 cgggggtgcc gagggcggcg gcgatgtcgg tgtaggaggg ctgctcgggt tggtagaaga
     1141 gcatggtcag gagcttccgg cagcgctcgt ccatctcggc gagggcggcg cggacgaggt
     1201 gctgctgttc gagtcgtgcg agcgcctcgt ccgggagcgg cccggcgtcg gggagcgaga
     1261 gcatctcgtc ttcgccttcg tcgcgttcgt cgtcgaaggg acgcatggac ttcgcgcggc
     1321 ggatgacgcg ccaggttttg cggcgcgcgg tcgtgacgag ccaggcgtgc aggcgcgagg
     1381 gctgctcgat ggtgtcgatg ctttgcagga gcgtggtgaa gacctcctga aagacttcgg
     1441 aggccgcgtc ctcgtccagc ccggcgcggc ggggaacggc gtagacgagc cgctggtaac
     1501 gtttgacgag agcctcccac gcgacctcgt cgccgcggcg gcaggccgcg acgagctgct
     1561 cgtccgtctg tcgcgttgcg tccgcgcccg tctgcatccc ggccagcata ggccgcgttt
     1621 tcttgctttg tccagcctgc ggagtttata aacatgaacg tgtgttcgtc tgcggtcgag
     1681 agaagggaaa tcatgttcat caggagattc gtcatcactc aggtcgtcgt gtggtgcgtc
     1741 gcggcgggcg gcgccgcggg gcaggagcgc gcgggcgcgg cggcgaccgg cggcgccgag
     1801 cagagagagg tggcggcgca gtcgagcgcg gaggaagggc ggctggtgag cgggtcgcgg
     1861 gcggcgatca tcgcggcggg cctgtcgccg gagttcttcg acgcgcactt ccggctcgaa
     1921 aaggtcttcg ccaaacccgc cgaccgtcgc gtcgtgtggc ggctcaaggt cggcgaatac
     1981 gagacgacgg tgaacgactc ggtcggctcc tacacggacg agaagggcga gcggcacgac
     2041 gcgcactcga tcacgggccg gctctcgccc gcgcgcgaca tcgggccgac gatcccgcag
     2101 aagcgcgccg agcgtttgat gaaggcgtgc atcggcgagt accagagcgg agcggtcgtc
     2161 taccagccgt tcggctcgcc gccgcgcgcc gcgctcgtct tcaccgccat gtccctgccc
     2221 gagccgcaga agcccgcggc gaaaggttca cagacgccgc ccgcgccgac gggcgccgcc
     2281 gccactactg cgccgacgct cgcgccgacg caagcggaca ccatccggca gggcggcaag
     2341 aagccgccgc cgctgcgcat cgggatggtc gatctcgaaa ccggtcggtg cgtggtcggc
     2401 gtcgggcagg tcggctcgcc gcacccgagc gcggagaagt acacgcgtcc ccgccgttga
     2461 ttaagcgccg cccgccgcgc gcgcgttcaa gcgcacgcac acacactctc tccgcgcacg
     2521 cgccgcgccc tttgcgcgag cggcgcgttt acatgactcc cgattggaaa agttgcggac
     2581 gggggggggg tgttttatga taacccccgc aagcccggcg gggcgtgaca cgccgtctat
     2641 cccttgcgcg gcgaagcccc cgggttcctg agctaccgca tcgcatcgga ggcgactgga
     2701 aggactgttc actacagggc cgcctcattc tttgcaaaca gcacacagat tttcctttga
     2761 agacggacgc cggtaggccc ctctcacgtc gtcgtggggg tagccgtgtg tccctctctt
     2821 gtcgagcacg ctgccgcgcg tcgtcgcccg cgggcgaggg cagggcgtaa tcacacaccg
     2881 ccatccggac acgatttgga aggagccacg tttcgatgag tagtcgtaga actccccgca
     2941 tcctacaaaa caggatgaaa cgcttcgtca cgcccgcgag cgtcatgacg ctcgcgctga
     3001 cgctcgcctg ccagagtctc ctgttggccc gcgcgccgtc agccgtcgcg cagaagagaa
     3061 ccgactcgaa gagcgttggc gtcgagctga gagacgcgga cgcgccgacg ggcgccgccg
     3121 cgcaggccgc gcaccggcag aagtccgcgc ccgtgaccgc cgcgcccgcg ccgcagtcga
     3181 agcaggccgg cgacgcggcg cggcccacct ccgtccgcgt gaccggcgtg cgcaccgtcc
     3241 gcgtcaactt caagtcactc gtcgcgctcg acgcgcgcaa ggccgccgtc aggagcaagc
     3301 agcccgcctt ccagcaagag attcactcgc cggggacgat ccccgaagtc ggcagcgagg
     3361 gcgcgtcgca ggcgtccgcc gaggacgacg cgaccctcgc gcccgccgcc gagccgcagg
     3421 acacgggcgg cccgctcgtg cccagccccg cacccgcgca gaacttcctc gcgcaggagg
     3481 acggcgcgaa ggtcggcacc ggcaccttca ccatcccgcc cgacacgatg ggcgccgtcg
     3541 gcttcgacaa ggtcttcgtc aacgtcaaca acaactaccg cgtccagaac aaggcgacgg
     3601 gcgcggcgct ctcgaccgtc tcgatcgaca ccttctggtc ttcgaccggc gcgaccggcg
     3661 tcttcgaccc gcgcatccag ttcgacccct accagaaccg ctggctgctc tcggcggtcg
     3721 cgaacgcgca gacggccaac tcgtccgtcc tcgtcggcgt ctcggacacc tccgacccgc
     3781 agggcaccta cacgctcttc cgcatcatcg tcggctgcgc gccgggcggc gcgggctgca
     3841 acgcgcaggg cgagtgggcc gacttcccga tgctcggctt caacaagaac tggctcgccg
     3901 tcggctggaa ccagttcacc atcaacacca gcgccttcgt cggcggcaag atgctcgtct
     3961 tggactaccc gacgctgcgc accggcaccg ccaccggcac gctcttcacc aacgcgagcg
     4021 ccgccaccgg cttctgcatg cacccggtga cgaccttctc ggccacagag gatacgctct
     4081 acgtgccgac gcaccagcag agcgcgggcg cgctctaccg cctccacaaa atcacgggca
     4141 cgcccggcgc gcccaccttc acgctcgacg caacggcgcg cgtgcgcacg ggcggcggct
     4201 ggacgcagcc cggcggcgac aacctgccgc agcagtgtgt gcccggcgtc ggagcgccga
     4261 cgcagacctg ccccgccacc ccgcgcggcc tcgaagcttc cgactccttc atccgctcga
     4321 acgcagtctt ccgcaacggg ctgatctatt acccgcaggc catcgcgctc cccgcgggcg
     4381 gcctcacggt cgcctcgcgc ttcgccgcgc agtggacggc gctcaacccc gacggcacct
     4441 tcaacacggg cgggcgcgtc gaggacgccg ccgcgcagat tttcaacggc ggcaagcatt
     4501 actcctaccc gtcgctctcg gtcaacaaga acaacgacat cctgctcggc ttctccgagt
     4561 tcgagtccga cgactacgtg gacgcgggct acaccttccg cctcggcacc gacgccgcgg
     4621 gcacgatgcg cgaccccgtc atctacaaag agggcgagga ctactaccag aagaccttct
     4681 cgggcacgcg caacaggtgg ggcgactaca gccacacggt tgttgacccc gtgaacgacc
     4741 gcgacctatg gacggttcag gagtacgcgc aactgcgcgt cgggacgacc ggtcaggggt
     4801 cgaacgactc gcgctggggc acttggtggg cgaagatcac ggcgcccgcg ggcgcgggcg
     4861 acctgctcat ctcggagttc cgcgtgcgcg ggccgaatcc gaacggcgcc ggcaacgagt
     4921 tcatcgaaat ctacaacccg aactcctccg cgctgaccgt cacgaccctc gacggcgcgg
     4981 cgggctacgc cgtcgccgcc tcggacggcg tcgcgcgctg cgtcatcccg agcggcacga
     5041 tcatccccgc gcgcgcacac tacctgtgcg tcaactcggt cggctactcg ctcgcttcgc
     5101 acccggcggg caacggcacc accgccaccg gcgacgccac ctacaccacc gacatccccg
     5161 ataatgccgg cctcgctatc ttcaacacct cggtcgccgc caacttcgcg ctcgccacac
     5221 ggctcgacgc cgtcggctcg acgagcgaag cgaacacgct ctacaaggaa gggacgggct
     5281 acccggcgct gacgcctttc tccatcgact actcgttcta ccgcgacaac tgcggcaagg
     5341 gcggctcgat caccacgctc ggcgtctgcc cgacgggcgg cttcccgaag gacacgaaca
     5401 acaacgcggc ggacttcatc ttcgtggaca cgaacgggac gagcgccgga gccggccagc
     5461 gtctcggctc gcccggcccc gagaacctct cgtcgcccgt ccagcgcaac agcagcttct
     5521 ccgtcccgct gctcgacacg tcggtcgggg gcgccatccc cccgaaccgc gtccgcgact
     5581 tcacgagcga cccggcgaac aactcgacct tcggcacgct caccatccgc cgccgcgtcg
     5641 tcaacaacac gggcgcgccg gtcacgcgcc tgcgcttccg cgtcgtcgat cagacgacct
     5701 tccccgcgcc ctcgggcttc gccgacctgc gcacgcgcac ctctacgctg gtcgtcgtct
     5761 ccggcgtcaa cgacccgacg acgtgtctcg cctcgaacgg cgtggcgacg acgccctgca
     5821 ccatcaacgt gcagggcacg acgctcgaac agccgccctc gcaactgaac ggcggcgcct
     5881 tcaacggcac ctactcggcg ggcaccatca cgctcggcac gccgctcgcc cccgg
//
LOCUS       DBNGOH010000355         4478 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4635949, whole genome shotgun sequence.
ACCESSION   DBNGOH010000355 DBNGOH010000000
VERSION     DBNGOH010000355.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4478)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4478)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4478
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4635949"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            206..577
                     /locus_tag="AC159P_06985"
                     /pseudo
     CDS             206..577
                     /locus_tag="AC159P_06985"
                     /inference="COORDINATES: protein motif:HMM:NF040425.6"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6444 domain-containing protein"
     gene            715..876
                     /locus_tag="AC159P_06990"
     CDS             715..876
                     /locus_tag="AC159P_06990"
                     /inference="COORDINATES: protein motif:HMM:NF024406.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695238.1"
                     /translation="MPARLRVTEHCAEVIRCPTCGRRTKAEFPDGVRATVQYGPTVLE
                     PVMNFDSTE"
     gene            1022..1453
                     /locus_tag="AC159P_06995"
                     /pseudo
     CDS             1022..1453
                     /locus_tag="AC159P_06995"
                     /inference="COORDINATES: protein motif:HMM:NF015038.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            1354..1644
                     /locus_tag="AC159P_07000"
                     /pseudo
     CDS             1354..1644
                     /locus_tag="AC159P_07000"
                     /inference="COORDINATES: protein motif:HMM:NF015038.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            1694..1933
                     /locus_tag="AC159P_07005"
                     /pseudo
     CDS             1694..1933
                     /locus_tag="AC159P_07005"
                     /inference="COORDINATES: protein motif:HMM:NF015038.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            2100..>4478
                     /locus_tag="AC159P_07010"
     CDS             2100..>4478
                     /locus_tag="AC159P_07010"
                     /inference="COORDINATES: protein motif:HMM:NF019136.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ligand-binding sensor domain-containing protein"
                     /protein_id="HIR3695239.1"
                     /translation="MRALRRRNLAAAPLALILVVLLPAARPRAEQLPIKTYTTADGLS
                     QDRVKRIVRDSRGFLWFCTADGLSRFDGYRFTSYDAARGLPHPSVNDLLETRAGTYWV
                     ATNGGGVARFHPSSRVEAGESGAKRFVAYAVGDTPRTNRVNKLYEDGDGRVWAGTDDG
                     LFLLEGDGAQFRRVGLGGAAPANSPVQVWDLLEDGERNLWVGTTAGLLRRTPDGRFAN
                     LPVEARGAKNVWGLMKDGEGRLWVSYAGGLLVLRPAPGVTTDVPPPWREVSRTEDDVR
                     SKRVLLPAAPGEAYRYTGADGLAGDDAQGSYRTRDGRIWVVTTAGLTELSGGLFRSYT
                     TAHGLIENTLLSLAEDRDGNVWIGTYSGGAMKLTLNGLTTYRREDGLSESVIRDIFED
                     QAGELCVAGRRSIDRFDGKKFTPVRPNLPADAATFDHVIQDHVGEWWFAAAGRLYRFP
                     RSHGVTGLAASRPRLYTAEDGLGGEGVQRLFEDSRGDIWIGTGGREVLSRWERATGQI
                     RRYSKADGLPEYSRPLSFSEDAKGGVWVGFREGGLARYRDGRFTALTSADGLPAGDIT
                     RVFSDAAGRLWAAVTFGGLVRIDDPDAERPRFRAYTIADGLTSNITRSIIEDRFGRLY
                     VGTTRSVDRVDPADGRVRHYTVADGLASNESNTSYADRGGALWFGTWNGLSRLVPAPE
                     RTTSAPPPVLIGGLSVAGEPRPLSELGETEVAGLLLGDRQNHVRIDFFGLSFAPGEVL
                     RYQYMLEGAGGDWGAPTDERSVTYANLRPGSYRFLVRAVNADGVASERPASVT"
BASE COUNT          774 a         1464 c         1515 g          725 t
ORIGIN      
        1 agtttatatt gctaacccgt aagttcagca tttccaagat ttctctgatt cagataatga
       61 ttattttacg aatgacaaat actgagggtg tctgttcagg aggtcgacta aactgcggaa
      121 aaaagcgact ttggcgacac cacgaagcta ttcccgtcgg ctataagccg tctcctctcg
      181 cgatgcctcc tccggaaggg cagtgatgcc gacctcctcc gagatctacc agacgtacct
      241 gcgcggcccg gtggccgtcc tccgcctctt cgagcagtct ttggggacac aggccatcta
      301 cgggccgccc gaccccgaca cgcagcagcg cacgatagat ggcctctcgg aggagatcgg
      361 gcagctcaag agccagatcg tccgcttgaa gatggagttg agtgaggcgc gcggcgacaa
      421 ccatcgcctc cggcggcgca acgccgaact cgaagcgctc atcggcaaag actctcataa
      481 ctcgtcgcgc ccgccctctt ccgacccgcc gtgggccaag cgcacgaaga gtctgcggcg
      541 gccctcgggc aagcttccgg gcgggcagcc cggtcacccc ggccacacgc tgcgcctgac
      601 catgaagccg acgcgcgtca tcacacaccg cccggcgcag tgccgtcact gcttgagtcc
      661 gctcgtggaa gccggagcac cggggccgcc gagcggcggc agatcatcga cctcgtgccc
      721 gccagactgc gggtcacgga gcactgcgca gaagtcatcc gctgcccgac gtgcgggcgc
      781 aggacgaagg ccgagttccc cgacggcgta agagccacgg tgcagtacgg cccgactgtc
      841 ctagagcctg ttatgaactt tgacagcacc gaatagctgt ttttcgggca tttttcgccg
      901 ttcgctctta cggcacaggc tctaggcgcc ctgtcagctg gtccacgcca caagatattg
      961 tggtacctac aggcttcatc acacatttcg ggacgaaagt tcataacagg ctctagcgcg
     1021 ggcgctctac ttgcacgact atcaactgct gtcttacgcg cggacagccg aagcgatgcg
     1081 tgagttgttc ggctgcgcgc tctccgccgg gacactctgg accgccgtcc ggcagtgcgc
     1141 ctcggggctg atcgaaacgg agttgaagat caagcgaggc ttgcggcgct ccgcagttat
     1201 ccacgccgac gagacggggc tgcgggtcga ggggcggctt cagtacgtgc atgtagcgag
     1261 tacgcctcgc ctgacgcact acggggcgga cgcgcggcgc ggcaaggcgg ccatcgacga
     1321 gatcggcatc tgccgcagta gcgcggcacc tgcgtccacg acgggtggcc ctcctccacc
     1381 ttctacccgc agtgtaagca cgcgctgtgc ggggcgcacc tgctgcgcga gctcaccttc
     1441 ttcgaggagc tgagtgagga gacgaaggcg tgggactctc ggctcaaaga gctgctgctg
     1501 gagatgaagg gggaagccga gagagtctgc gtcggaggcg gcaagcggct cgtcgcggag
     1561 cgtcttgagg agctaacagc cagccatgac gagccggtga ccgaagggct gcgggcgccg
     1621 ccgccccctg atgtaccgga gtaggtgagg aaacaggcgc gcaacctgct gctgcggctg
     1681 gagaagaaag gaagaagtgc tgcggttcat gaccgacttc cgcgtgccgt gggacaacac
     1741 ccagacggag cgagacctcc ggaaggggaa gctacagcag aagttcggcg ggtgcttccg
     1801 cagcgaggaa ggtgcgcgcc ggttctgccg caatcggggt tatatctcca ccgtgcagaa
     1861 gcagggtcaa ggtgtgctgg gagcgctcgc gggggcgtgc cagagagcgt cgctaagtga
     1921 tagaatacgc tagggataga tgaatagttt catcatgccc gcgcaacaga ctcgcgggcc
     1981 ggccgtgatc ccttttgcgc acgaattacg cattaaaaat cagaggatta ggcgcggcct
     2041 tcgtcaggcg cgcatacccg cgtattcatc gcctcggcga cggtctgatg gtttccctga
     2101 tgagggcatt acgacggcgg aacctcgccg cggcgcctct ggcgcttatc ctcgtggtac
     2161 tgctcccggc ggcacggccc cgcgccgagc aactgccgat caagacttac acgacggctg
     2221 acggcctctc gcaggatcgc gtcaagcgca tcgtgcgcga ctcgcgcggc ttcctctggt
     2281 tctgcaccgc cgacggcctc tcgcgcttcg acggctaccg gtttactagc tacgatgcgg
     2341 ctcgcgggct gccgcaccct tccgtcaacg acctgctaga gactcgggcc ggaacgtact
     2401 gggtggccac caacggcggc ggcgtggccc gcttccaccc gtcgtcccgc gtagaggcgg
     2461 gggaatcggg cgcgaagcgc ttcgtggcct acgccgtggg tgacacgcca cggaccaacc
     2521 gcgtcaacaa gctttacgag gacggcgacg ggcgcgtctg ggcgggcacg gacgacggcc
     2581 tctttctgct ggagggcgac ggggcgcaat tccgccgcgt cgggctcggc ggcgcggccc
     2641 cggcgaattc accagttcag gtctgggatt tacttgaaga cggggagcgc aacctctggg
     2701 tcgggacgac ggccggcttg ctgcgccgga cgccggacgg acggttcgcg aacctgcccg
     2761 tggaggcgcg cggcgcgaag aacgtctggg gcctgatgaa ggacggcgaa ggccgcctgt
     2821 gggtgagcta cgcgggcggg ctgctcgtct tgcgccctgc gcccggggtg acaacggacg
     2881 tgcctccgcc ctggcgggag gtgagccgga cggaggatga cgtgcggagc aaacgcgtcc
     2941 tcttacccgc cgcgccgggc gaagcctacc gctacacggg cgccgacggg ctggccggcg
     3001 atgacgcgca gggatcatac cgtacgcgcg acgggcggat atgggtcgtc acgacggcag
     3061 ggctgacgga actgtccggc ggcctcttcc gcagctacac gacggcgcac ggcctgattg
     3121 agaacaccct cctctcgcta gccgaagacc gtgacggcaa cgtctggatc ggcacctact
     3181 cgggcggcgc gatgaagctc acgctcaacg ggctgacgac ctaccgccgc gaggacggtc
     3241 tcagcgagtc ggttatcagg gacatctttg aagaccaggc cggcgagttg tgtgtggccg
     3301 gaaggcggtc catcgaccgc ttcgacggga agaagttcac cccggtccgc cccaacctgc
     3361 cggccgacgc agccaccttc gaccacgtca tccaggatca tgtgggggag tggtggttcg
     3421 ccgccgcggg ccgcctgtac cgcttcccac gctcgcacgg cgtcaccggc ctcgccgcct
     3481 cacgcccccg cctctacacg gccgaggacg ggctgggcgg cgagggggtg cagcgcctct
     3541 ttgaagattc gcggggcgac atctggatcg gcaccggcgg gcgggaagtg ttgagccgct
     3601 gggagcgcgc gaccggacag atccgccgct actcgaaggc cgacgggctg ccggaataca
     3661 gccggccgct ctccttcagt gaggacgcga agggcggcgt ctgggtgggt ttccgcgagg
     3721 gggggctggc tcgttaccgc gacggccgct tcaccgcgct gacgagcgcg gacggattgc
     3781 ccgccggtga catcactagg gtgtttagcg acgccgccgg gcgcctctgg gcggcggtca
     3841 ccttcggcgg ccttgtccgg atcgacgacc cggatgccga gcgcccgcgc ttccgcgcct
     3901 acacgattgc cgacggcctg acgagtaaca tcacccgcag catcatcgaa gaccgtttcg
     3961 gccgcctgta cgtcgggacc acgcgcagcg tcgaccgcgt cgacccggcg gacggccgcg
     4021 ttaggcacta cacggtcgcc gacggccttg ccagcaacga gtcgaacaca tcttacgccg
     4081 accgcggcgg cgcgctgtgg ttcggcacct ggaacgggct ctcgcggctg gtgccggcgc
     4141 cggaacggac tacgtctgcg ccgcccccgg tcctgatcgg cggcttaagc gtcgccggcg
     4201 agccgcgccc actctccgag ctgggcgaga cggaggtcgc gggtctgctc ctcggagaca
     4261 ggcagaatca cgtgcggata gacttcttcg gcctctcttt cgcacccggc gaggtgctgc
     4321 gctaccagta catgctcgaa ggtgcgggcg gcgactgggg cgcgccgacc gacgagcgca
     4381 gcgtcactta cgctaacctc cgccccggct cgtaccgatt cctcgttcgc gcggtgaacg
     4441 ccgacggcgt cgcgagcgag cgccccgcct ccgtgaca
//
LOCUS       DBNGOH010000356         6501 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3353851, whole genome shotgun sequence.
ACCESSION   DBNGOH010000356 DBNGOH010000000
VERSION     DBNGOH010000356.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6501)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6501)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6501
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3353851"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..503
                     /locus_tag="AC159P_07015"
     CDS             <1..503
                     /locus_tag="AC159P_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012098911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR3695240.1"
                     /translation="LYIALKSVRSALLVMANLPLALIGGVVMVFLSGGTLSVASLVGF
                     ITLFGIATRNGIMLISHYSHLMVEEGVSFRESIVQGSMERLSPILMTALVTGVGLIPL
                     ALGANEPGKEIQQPMAVVILGGIVTSTFLNMIVIPALYLKYGRAEAHAPSRDYPGGRE
                     FAPAGD"
     gene            692..1846
                     /locus_tag="AC159P_07020"
     CDS             692..1846
                     /locus_tag="AC159P_07020"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004308 - exo-alpha-sialidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HIR3695241.1"
                     /translation="MRESESPSGPDSREPELFSTADGRVILSWVEKVGGGRHALRFAV
                     RDAAGWSEARTVSEGENWFVNWADFPSVIALADGSLAAHWLVKSGPGTYAYDVNISRS
                     KDGGKTWGRPLVPHTDGTRTEHGYVSLIPLADGRLGVVWVDGRETKGVKEGHEENEPL
                     PVSMQLRYAAPGAAGKPSDEAVLDGRICECCQTSAAMTSEGPIAVYRDRSQEEVRDIY
                     FVRRLGDGWSEPRPVHADGWQINGCPVNGPSVAADGRRVAVAWYTEAGDSPRVQLAFS
                     GDAGASFGPPVRVDDGEAMGRVDVVLLADGSALVCWMAGGAEGGANKVRRVGTDGTLG
                     PVAVIARTDVSRSSGFPRMARLGDTVYFAWTQFGRPSQVHTATADVSAFK"
     gene            complement(1966..3840)
                     /locus_tag="AC159P_07025"
     CDS             complement(1966..3840)
                     /locus_tag="AC159P_07025"
                     /EC_number="1.1.3.9"
                     /inference="COORDINATES: protein motif:HMM:NF020683.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose oxidase early set domain-containing
                     protein"
                     /protein_id="HIR3695242.1"
                     /translation="MEERENHEHHEHTDKEHKGDGPKLDLKTQRRILRFVNGARSPDQ
                     LALAPELVVDLHAEHRQRLQPDPHDIHEGLEHERARLLKTETAKRLIEQRDRLSPVFG
                     FLNLEELLRDFEVFIRDLAALLSAATLGEWQGPFDIPAEFDRPIHAALLKTGDVLFYG
                     GLPTGKNTFRYTPDPSGGLGTFTLVASVPGDSLFCAGQVFLSDGKLLVAGGGGDGTGA
                     RHNHSWLFDPDTDSWSRTGDLNEFRWYPTIINLGDPQGRMLVVGGLGGGSDIAQPEIY
                     REDTGVCERVWGPGGVGDTSANHRFQQLYPGFNALPGGEVFYSPTGWHSGGCSGAADF
                     PQALPSGYYKILSATPPFTATWTDVGAVDVIAEATLDRVKGMSVLLLQQTYPYVQVLV
                     AGGGKDPESTTTFQMINLSTLSPEWGPALPLPDGLSRANVNLVLLPDGKVFLSGGRPL
                     TGTPPDAGECWVYDPLIMTWSQMDRMANMRGYHSLAMLLPDARVLTAGNQCPPDRSIE
                     IFSPPYLFAPDGSPAPRPVITNCPDHVHHGGHFCIETPYPCAVAKVVLVRPSALTHQT
                     DSEQRVIPLTHSVSGPTALDVAAPDGWLPHALAPRGWYMVFLIDTAGVPSVAHFIHLT
                     "
     gene            5034..5453
                     /locus_tag="AC159P_07030"
     CDS             5034..5453
                     /locus_tag="AC159P_07030"
                     /inference="COORDINATES: protein motif:HMM:NF024515.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HIR3695243.1"
                     /translation="MSKTIATLGVIAVLLAAAACGSGRAGSETKEIRSGKVGALTVTI
                     SNAEGVMKHGDGEFTVTFKDASGKPVDVGAASLNFYMPSMGTMPVMNNPAALATTDTP
                     GVYRAKAKIEMAGEWQAQVAYEGPAGSGKGSFPVTAQ"
     gene            5492..>6501
                     /locus_tag="AC159P_07035"
     CDS             5492..>6501
                     /locus_tag="AC159P_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015720391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR3695244.1"
                     /translation="MINRLIEISMRYRAVVVAVYLGLAFWGYWALLSTPIDAIPDLSD
                     NQVIVFTDWPGRSPQEVEDQVTYPLVTSLQGLPGVRTVRASSAFSFSMINVIFEDDVE
                     LYWARTRVLERLNLVTGQLPQGVKPTLGPDATGVGQIFWYTLESDTHSLRDLRTLQDW
                     FVRYQLNSVPGVAEVASVGGYVQQYQVDVDPNRLRAYGLPLSGVVEAVERSNTNVGGN
                     VIEQSGQWAVVRGIGLVESVADVENIVVGAEKGTPIYVRNVASVKLGNAFRTGALDKN
                     GREAVGGVVVARYGVSVLEVIESVKRKLEELKPGLPEGVRVVPFYDRTQLINRATDTL
                     KWA"
BASE COUNT         1050 a         2173 c         2206 g         1072 t
ORIGIN      
        1 tgctctacat cgcgctgaag tccgtccgct cggccctgct tgtcatggcg aacctcccgc
       61 tcgcactcat cggcggtgtg gtgatggtct tcctgtcggg cggaacgctc tccgtcgcct
      121 cgctcgtcgg tttcatcaca ctcttcggca tcgccacccg caacggcatc atgctgatca
      181 gccactactc gcacctgatg gtcgaggagg gcgtgtcttt ccgcgaatcg atcgtgcagg
      241 gctcgatgga gcgcctctcg cccatcctga tgacggcgct cgtcaccggc gtcggcctca
      301 tccccctggc gctcggcgcg aacgagccgg gcaaggagat ccagcagccg atggccgtcg
      361 tcatcctcgg cggcatcgtc acctcgacgt tcctcaacat gatcgtcata ccggctcttt
      421 atctgaagta cgggcgcgcc gaggcccacg cgccgtcacg cgactacccc ggcgggcggg
      481 agttcgcgcc ggcgggcgat taaggtatct tccagcgccg gtcattacat aacgagtagg
      541 ggtacatcct ctaatgatgg tgatcacccg tagtattcaa atactggtgt tactggcagc
      601 ggccaccgtc tccggctgcg acattggtgg tgccgggcct acgctctccc aggccacgcc
      661 gacaccgccg cccgcaggcc cggccctaga ggtgagagag agcgaatccc cgtcggggcc
      721 ggacagccgc gagccggaac tcttctcaac cgctgacggg cgcgtcatcc tgagttgggt
      781 cgaaaaagtc ggcggggggc ggcacgcgct gcggttcgcg gtgcgcgacg ccgccggctg
      841 gtccgaagcc cgcaccgtgt cggagggcga gaactggttc gtcaactggg cggacttccc
      901 gtcggtcatc gcgctcgcgg acggctctct ggccgcgcac tggctggtca agagcgggcc
      961 gggcacctac gcgtacgacg tcaacatctc gcggtcgaaa gacggcggta agacttgggg
     1021 gcggcccctc gtcccgcaca cggacggcac gcggaccgag cacggctacg tctcgctgat
     1081 cccgctcgcc gacgggcgcc tcggcgtggt gtgggttgac gggcgcgaga cgaagggcgt
     1141 gaaggagggg cacgaggaga atgagccgct gcccgtcagc atgcagcttc gctacgcggc
     1201 cccgggcgcc gccggcaagc cctcggacga ggcggtgctc gacgggcgca tctgcgagtg
     1261 ctgccagacc tctgccgcga tgacttcgga agggccgata gccgtctacc gcgaccgctc
     1321 gcaagaggag gtgcgcgaca tctatttcgt ccgccggctg ggcgacggct ggagcgagcc
     1381 gcgccccgtc cacgccgacg ggtggcagat caacggctgc cccgtcaacg ggccgtcggt
     1441 cgcggcggac gggcggcgcg tcgccgtcgc gtggtacacg gaggcgggcg actcgccccg
     1501 cgttcagttg gccttctcgg gcgacgccgg cgcgagtttc gggccgcccg tgcgggtgga
     1561 cgacggcgag gcgatgggac gggttgatgt cgtgttgctg gcggacggct cggccctggt
     1621 ctgctggatg gcgggcgggg ccgaaggcgg cgcgaacaag gtgcgtcgcg tcggcacgga
     1681 cggaacgctc ggcccggtcg cggtcatcgc gcggaccgac gtttcccgct ccagcggctt
     1741 cccgcgcatg gcccgcctcg gcgacaccgt gtacttcgcg tggacgcagt tcggtaggcc
     1801 ctcacaggtt cacacggcca ccgccgacgt cagcgccttc aaatagcccg ccgcgtgccg
     1861 cacgactacg gggtttcaaa acatataacg gggccgcagt cgaaccgccg ggtgacggcc
     1921 gcgagtgcaa actacgcacc cgcccgacac accgaacgcc gcggctcagg tcaggtgaat
     1981 gaagtgcgcc accgagggca cacccgccgt gtcgatcagg aagaccatgt accagccccg
     2041 aggcgcgagc gcgtgcggga gccagccgtc gggcgccgcg acgtccagcg ccgtcgggcc
     2101 gctcaccgag tgggtcagcg gaatcacccg ctgctcgctg tcggtctggt gggtcaacgc
     2161 cgacgggcgc accagcacga ccttcgcgac ggcgcacggg tacggtgtct cgatgcagaa
     2221 atgtccgccg tgatgcacgt ggtcggggca gttcgtgatc acagggcgcg gcgccgggct
     2281 gccgtccggc gcgaacaggt agggcgggct gaagatctcg atgctgcggt cgggcgggca
     2341 ctggttgccc gccgtcagca cgcgcgcgtc gggcaggagc atggcgagcg agtggtagcc
     2401 ccgcatgttg gccatgcgat ccatctgcga ccaggtcatg atcaacgggt cgtagaccca
     2461 gcactcgccg gcgtcgggcg gcgtccccgt gagcggcctg ccgccggaga ggaagacctt
     2521 cccgtcaggc agcaggacga ggttgacatt ggccctcgac agcccgtccg gcagcggcag
     2581 ggccggcccc cactccggcg acagcgtgga gaggttgatc atctggaagg tcgtggtgct
     2641 ctccgggtcc ttgccgcccc ccgccaccag cacctgcacg taggggtagg tctgttggag
     2701 gaggagcacg ctcatccctt tgacccggtc gagcgtcgcc tcggcgatca cgtccacggc
     2761 ccccacgtcg gtccaggtcg cggtgaacgg cggcgtggcc gaaagaatct tgtagtagcc
     2821 cgaaggcagc gcctgcggga agtccgccgc ccccgagcag ccgccgctgt gccagccggt
     2881 cgggctgtag aagacctcgc cgcccggcag ggcgttgaag ccggggtaga gctgctggaa
     2941 tctgtggttg gcgctggtgt cgcccacgcc gccgggcccc cagaccctct cgcacacgcc
     3001 tgtgtcctcg cgatatatct ccggctgggc gatgtcggag ccgccgccca gcccgccgac
     3061 gacgagcatc cggccctgtg ggtcgccgag gttaatgatc gtgggatacc agcggaactc
     3121 gttcaggtcg cccgtccgcg accaactgtc ggtgtcgggg tcgaagagcc atgagtggtt
     3181 gtgccgcgcc cctgtgccgt cgccgccgcc gcccgcgacc agaagcttgc cgtccgacag
     3241 gaacacctgc ccggcgcaga acagcgagtc gcctgggacg ctcgccacca gcgtgaaagt
     3301 gccgagcccg ccggacgggt ccggcgtata ccggaaggtg ttcttcccgg tcggcagccc
     3361 gccgtagaac agcacgtcgc ccgtcttcag gagcgccgcg tggatcgggc ggtcaaactc
     3421 ggcggggatg tcgaacggcc cttgccactc gcccaacgtg gcggcggaga ggagtgcggc
     3481 gaggtcgcgt atgaacactt caaaatccct gagcagctcc tccagattca gaaaaccgaa
     3541 gacggggctc aggcggtcgc gctgctcgat cagcctcttc gccgtctcgg ttttcaggag
     3601 cctcgcccgt tcgtgctcca agccttcgtg gatgtcgtgc gggtcgggtt ggaggcgctg
     3661 gcggtgctcg gcgtgaaggt cgacgaccag ctcgggcgcg agtgcgagtt ggtctggaga
     3721 gcgggcgccg ttgacgaacc gtaggatccg ccgctgggtc ttcaggtcga gcttcgggcc
     3781 gtcgcccttg tgctccttgt ccgtgtgctc gtggtgctcg tgattctctc gttcctccat
     3841 gatgaggtct cctttacctt gtgccccgcc actaccccgc agaggcttct tgccgagcct
     3901 tctcacgaag gccaaagccg aaacactcgg cgccgacgcg taatggcgag gagaaagcag
     3961 aagcgccggg ctgctcgacc ctccgctgcg cgggtcgccc gttcacttgt caccctcgtg
     4021 tgaaaccacc gattgtcagc cggagtaggg gcaaggttat gttctcgcga taccgctgtt
     4081 gaggttaacg ggcgaggcca cgcaagcggg aaccgggggt aagtgcggga aggatatgcc
     4141 tgagccttac gggtgtcaat gaaaaataga ggcggcagcc ctcatcaact cccacgggcg
     4201 cctccgggtg agagcatacg gctcgtggct taaatgctgc gggttttccc acaacggcag
     4261 gggtaaattc cgcatccgag ttctttcatc cggcggaatc aggtataatt ccgcccggca
     4321 cgtatgttgc tccgcttgtt gtaaagaccc ttcccgcatt acgcggtcgg ggtaaacggc
     4381 ctcgatttta gaagcggcga tgctgaaaag gctgacatca ttactccttc tcctgtcggt
     4441 cggcgctggc gtggcctcgg gcgcaccgct gcactccggg gggcgtgagt gtgacatgga
     4501 agcttccatg gggatgatgg actgctgtaa gcgcgcccgc ttaaacgggg acacccccgg
     4561 agtccaggca gcccgcctgt gctgtgccat caactgccag aacggtaacg cgaacggacc
     4621 cgcctcggcg gcccgcgtca cgccgcccca ggccgccccg acacacccgg ccgccgcgcc
     4681 ggcacccgtc gcgctcatgg tcccccgcgg gccgcgacgc gtcggcccgt cgcgcccgga
     4741 cgaatccccg cccgtctatc ttctcaatct cgcatttctg atctgatgcc ctgtaggcag
     4801 tagtgtgtct gcccgcctcg ccttaaccgg cgaagccggg gcaggttgtt gcgtccgaaa
     4861 gcgcctccga gggtagtcta ccggagacgc gcgcctcggt ctcactcccg gcccggtgca
     4921 gactactacg actgctaacg gggcggacgt gtgtctcgcg gtcggcgttc gacccctccg
     4981 cgcccccgga cacgcggaca catcaaccaa tcacagatca ggagattcaa cccatgagta
     5041 agacaatcgc caccctcggc gtcatcgcag tccttctcgc cgccgcggcc tgcggctccg
     5101 gcagggccgg ctcggagacg aaagagatca ggagcggcaa ggtcggcgcc ctgacggtca
     5161 ccatctcgaa cgcggaaggg gttatgaagc acggcgatgg ggagttcacc gtcaccttca
     5221 aggacgcctc gggcaagccc gtggacgtcg gggccgcttc gctcaacttc tacatgccct
     5281 cgatgggcac gatgcctgtc atgaataacc cggcggcgct tgcaacgact gacacgcccg
     5341 gcgtctaccg ggcgaaggcc aagatcgaga tggcgggcga gtggcaggcg caggtcgcat
     5401 acgaaggccc ggccggcagc ggcaaaggca gtttccccgt cacggcgcag tagcgcgggg
     5461 gcggggcttc gcgcgccccg atagacttga gatgattaac cggctcatag aaatatcgat
     5521 gcgctaccgg gcggtcgtcg tcgccgtcta cctcggcctc gccttctggg gctactgggc
     5581 gctgctctcg acgccgattg acgccatccc cgacctctcg gacaaccagg tcatcgtctt
     5641 caccgactgg ccggggcgct cgccgcagga ggtcgaggat caggtcacgt acccgctggt
     5701 gacgagcctg caaggcttgc cgggcgtgcg gacggtgcgc gccagttcgg ccttcagctt
     5761 ctcgatgatc aacgtcatct tcgaggacga cgtcgaactc tactgggcgc gcacccgcgt
     5821 gctcgaacgc ctgaacctcg tcaccggcca actgccccag ggcgtgaagc cgacactcgg
     5881 ccccgacgcg accggcgtcg gccagatctt ctggtacacg ctggagagcg acacgcattc
     5941 cctgcgcgac ctccgaacct tgcaggactg gttcgtgcgc taccagttga actccgttcc
     6001 cggcgtcgcc gaggtcgcga gcgtgggcgg ctatgtgcag cagtaccagg tcgacgtcga
     6061 cccgaatcgc ttgcgcgcct acgggctgcc gctctcaggc gtggtcgagg cggtcgagcg
     6121 ctcgaacacg aacgtcggcg ggaacgtcat cgagcagtcg gggcagtggg ccgtggtgcg
     6181 cgggatcggc ctcgtcgagt cggtggccga cgtcgagaac atcgtcgtcg gggcggagaa
     6241 aggcacgccc atctacgtcc ggaacgtcgc ctcggtgaag ctcgggaacg ccttccgcac
     6301 gggcgcgctc gacaagaacg gcagagaggc cgtgggcggc gtggtcgtcg cgcgctacgg
     6361 cgtgagcgtc ctcgaagtca tcgagtcggt caagcgcaag ctcgaagagt taaagccggg
     6421 gctgcccgag ggcgtccgcg tcgtgccctt ctacgaccgc acgcagctca tcaaccgcgc
     6481 caccgacacc ttgaagtggg c
//
LOCUS       DBNGOH010000357         5298 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4087095, whole genome shotgun sequence.
ACCESSION   DBNGOH010000357 DBNGOH010000000
VERSION     DBNGOH010000357.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5298)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5298)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5298
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4087095"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(22..1446)
                     /locus_tag="AC159P_07040"
     CDS             complement(22..1446)
                     /locus_tag="AC159P_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006233677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3695245.1"
                     /translation="MQQKPRLSFWQIWNMSFGFLGIQFGWGLQLANMSPIYRYLDAEE
                     STLPYLWLAGPITGLLVQPIIGSMSDRTWNRLGRRRPYFLVGALLASAALFVMPNSSA
                     LWMAAGLLWILDASINITMEPFRAFVADKLPEEQRTTGFVMQSFFIGIGQTLANALPL
                     ILTAAGVTGVLASGIPLSTDYAFKIGAFAFLIAVLVTVVTTKEYPPEDMEAFERMRRE
                     HAGLMGGFREIFLSIRDMPTTMKQLAVVQFFTWFALPCMWQFFGLTVARHVYGAAGPE
                     DAGFREGTEWGGLCFAVYNVVCFLIAFLLPAMARAASRKGVHLIALLCGGLGLLSVSF
                     AGPGDRYFLWLSMAGVGVAWASILSMPYVILSGSVPAHRMGVYMGVFNLFIVIPQVVM
                     SFLTPYLIRHVLGTDPIYVVMLGGLSMLVAAVSVLVVRDEPRPVSVEDVLEADEHTPL
                     TTTLSAQPVPSTGLVDDEGRGDTR"
     gene            complement(1537..2262)
                     /locus_tag="AC159P_07045"
     CDS             complement(1537..2262)
                     /locus_tag="AC159P_07045"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR3695246.1"
                     /translation="MPLKTKPLSEQVALVTGGGTGIGRAFAESLSREGARVVIASRRG
                     EVLKRTADELNAAAGAERVFAYAFDVRVRAECEAVVRYAVERFGALDILINNSGLAVP
                     ETVEEITDEGWDKVSETNVKGAMWLVRAALPSMLAADFGDVVNVSSQAGKHGYADVPS
                     YCASKHALLGFAESLRDHVRKTGANVRVFNFCPGLVDVENAAADAPPRPGFIHVSNMA
                     RTLLYALSLDRDAVLEDIGLYAK"
     gene            complement(2268..4187)
                     /locus_tag="AC159P_07050"
     CDS             complement(2268..4187)
                     /locus_tag="AC159P_07050"
                     /inference="COORDINATES: protein motif:HMM:NF012356.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HIR3695247.1"
                     /translation="MKHTARLVSVSGFLVILCAVTCAAQNASAVSKVEPPSWWAGHSI
                     NPVRVMLRGRNLHGARVEPVGAGIGTGLVRVNERGTYLFVDVTIDERAAAGTRRLRVV
                     TAAGAVEAPFEIVPPIPRAGRYQGFTTDDVIYFIMPDRFADGDPSNNNPAKSPGLLDR
                     TKGRYYHGGDFQGIIDRLPYLKDLGVTALWINPVYDNTDRLDEREMYPEVEGGPKRPT
                     TAYHGYGAIDFYGVEEHYGDMKKLRELVDKAHAAGLKVIQDQIANHTSPYHPWATERP
                     TPTWFYGTVENHLSNNWQKWTTMDPNATRQTQRRNLEGWFIDILPDLNQDDEEVRRYL
                     IQNTLWWLGQVGFDAIRMDTLPHVPRHFWRDWSAAIKREYPQVNILGELFDGDPALLA
                     YFQKGRVGHDGLDTGIDTLYDFALFYAVRDSFAKGESVRKLANVLAHDYLYPNPRALV
                     PFLGVHDMQRFMNEQGATTEGLKLAQTFIMTTRGTPLLYYGDELAMPGGGDPDNRRDF
                     PGGFAGDPRNAFTNEGRTATQNDVFNHVRRLARLRVELPALRHGSVLHLYDEEQQTAF
                     ARVHGNQTVIVAFNNDTKPATFEIGLAENGPGNNARLVDRLEVVKGELRVENNALKVT
                     LPARSASIFTERENR"
     gene            complement(4184..>5298)
                     /gene="malQ"
                     /locus_tag="AC159P_07055"
     CDS             complement(4184..>5298)
                     /gene="malQ"
                     /locus_tag="AC159P_07055"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014101426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HIR3695248.1"
                     /translation="GAAWNQWEAPMVRRLPEALAAAREGLRDEIDAHKFYQFLFFRQW
                     SRLKAYANDKGIRIVGDIPIFVAHDSSDVWAQPEQFHLNADGSPRVVAGVPPDYFSKT
                     GQLWGNPLYDWEHMRADGFGWWIERASATLKLVDILRIDHFRGFAAYWEVPGGDQTAE
                     RGRWVPAPGRELFQAIRDALGVLPIVAEDLGVITPDVEALRDDFGFPGMRILQFGFSS
                     DARNKDLPHNYVPNSVVYTGTHDNDTIVGWFNSQAGTGSTRSAEQIERERDFCLSYLA
                     TDGAEIHWAFIRAALASVSDTCLMPLQDVLGLGTEARMNLPASTSGNWDWRFRPEMLT
                     PQIAARLQYFTRLYGRDPQPEPTAHHEHHAGEADAS"
BASE COUNT          857 a         1706 c         1859 g          876 t
ORIGIN      
        1 agcccctcgc tcgtcccgca ctcaacgagt gtcgccccgg ccctcgtcgt ccacgagccc
       61 cgtgctcggg acgggctgcg ccgagagcgt cgtcgtgagc ggcgtgtgct cgtcggcttc
      121 gagcacgtcc tcgaccgaca cggggcgcgg ctcgtcgcgc acgacgagga cggagaccgc
      181 cgccaccagc atgctcaggc ccccgagcat cacgacgtag atggggtctg tgcccagcac
      241 gtggcggatg aggtagggcg tgaggaacga catcacgacc tgcgggatga cgatgaagag
      301 gttgaagacg cccatgtaga cgcccatccg gtgcgccggc accgagcccg aaagtatcac
      361 gtagggcatc gacagaatcg acgcccacgc gacgccgacg cccgccatcg agagccagag
      421 gaagtagcgg tcgccggggc cggcgaagga gaccgagagg agcccgagcc cgccgcagag
      481 gagcgcgatg aggtggacgc ccttgcggct cgcggcgcgg gccatcgccg ggagcaggaa
      541 ggcgatgagg aagcagacga cgttgtagac ggcgaagcac aagccccccc actccgtccc
      601 ctcgcggaag ccggcgtctt cggggcccgc ggcgccgtag acgtggcgcg ccaccgtcag
      661 cccgaagaac tgccacatgc agggcagcgc gaaccacgtg aagaactgga cgacggcgag
      721 ctgcttcatc gtcgtcggca tgtcgcggat cgacaagaag atttcgcgga agccgcccat
      781 cagccccgcg tgctcgcgcc gcatacgctc gaacgcctcc atgtcttcgg gcgggtactc
      841 tttggtcgtc acgaccgtca ccagcacggc gatgaggaag gcgaaggcgc cgatcttgaa
      901 ggcgtagtcg gtcgagagcg ggatgccgct ggccagaacg ccggtcacgc ccgccgccgt
      961 caggatgagc ggcagcgcgt tcgccagcgt ctgcccgatg ccgatgaaga agctctgcat
     1021 gacgaagccg gtcgtgcgct gctcctcggg cagcttgtcg gcgacgaagg cgcggaacgg
     1081 ctccatcgtg atgttgatgc tcgcgtccag aatccacagc agccccgccg ccatccacag
     1141 cgccgaagag ttcggcatca cgaacagcgc cgcgctcgcc aggagcgcgc cgacgaggaa
     1201 gtagggccgc cgccgcccca gacggttcca cgtccggtcg ctcatcgagc cgatgatcgg
     1261 ctgcaccaag aggcccgtga tcggccccgc cagccagagg tacggcagcg tgctctcctc
     1321 cgcgtccagg tagcggtaga tgggactcat gttggcgagc tgcaagcccc acccgaactg
     1381 gatgccgagg aacccgaaac tcatgttcca gatttgccag aagctcagac gcggtttctg
     1441 ctgcatggct tatcctccga aatgatgaat gcggattgat gaatgatgaa tgaaagacgg
     1501 cgtgctttca attcatcatt ccgcattcat cgttcatcat ttcgcgtaaa ggccgatgtc
     1561 ttcgagcacc gcgtcgcggt cgagcgagag cgcgtagagg agcgtgcgcg ccatgttcga
     1621 gacgtggatg aagccgggcc gcggcggcgc gtccgcggcg gcgttctcga catcgacgag
     1681 gccggggcag aagttgaaga cgcggacgtt ggcgcccgtc ttgcgcacgt ggtcgcgcag
     1741 ggattcggcg aagccgagca gcgcgtgctt cgacgcgcag tacgaaggca cgtccgcgta
     1801 cccgtgcttg cccgcctgcg aagagacgtt caccacgtcg ccgaagtccg cggccaacat
     1861 cgacggcaac gccgcccgca cgagccacat cgcgcccttc acgttcgtct ccgacacctt
     1921 gtcccagccc tcgtccgtaa tctcctcgac cgtctccggc accgccagtc ccgagttatt
     1981 aatcaggatg tcgagcgcgc cgaagcgctc gaccgcgtag cgcacgaccg cctcgcactc
     2041 ggcgcgcacg cgcacgtcga acgcgtaagc gaagacgcgc tcggcgcccg ccgccgcgtt
     2101 caactcgtcc gccgtccgct tcaacacctc cccgcgccgc gaggcgatga cgacccgcgc
     2161 gccctcacgc gacaggctct cggcgaacgc gcgaccgatg cccgtgccgc cgcccgtgac
     2221 gagcgcgacc tgttccgaca gaggtttggt cttgagcggc atcgtcttta acggttctcg
     2281 cgctcggtga aaatactcgc ggagcgcgcg ggcagcgtca ccttcagcgc gttgttctcg
     2341 acgcgcagtt cgcccttgac gacttcgagg cggtcaacga ggcgcgcgtt gttaccgggg
     2401 ccgttctctg ccagtcctat ctcgaaggtc gcgggcttgg tgtcgttgtt gaaggcgacg
     2461 ataacggtct ggttgccgtg gacgcgcgcg aaggcggtct gctgctcctc gtcgtaaagg
     2521 tggagcacgg agccgtggcg gagcgcggga agttcgacgc gcaggcgtgc gagccgccgc
     2581 acgtggttga agacatcgtt ctgcgtcgcc gtgcgcccct cgttcgtgaa ggcgttgcgc
     2641 gggtcgcccg cgaagccgcc ggggaagtcg cggcggttgt cggggtcgcc gccgccgggc
     2701 atggccagct cgtcgccgta gtaaaggagc ggcgtgccgc gcgtcgtcat gatgaaggtc
     2761 tgcgcgagct tcaagccttc cgtggtcgcg ccctgctcgt tcatgaagcg ctgcatgtcg
     2821 tggacgccga ggaacgggac gagagcgcgc gggttcgggt agaggtagtc gtgcgcgagg
     2881 acgttggcga gcttcctgac ggactcgccc ttggcgaacg agtcgcgcac cgcgtagaag
     2941 agcgcgaagt cgtagagcgt gtcgatgccc gtgtcgaggc cgtcgtgccc gacgcgccct
     3001 ttctggaagt aggcgaggag cgcggggtcg ccgtcgaaca gctcgccgag gatgttgacc
     3061 tgcgggtact cgcgtttgat ggccgcagac cagtcgcgcc agaagtggcg cgggacgtgc
     3121 gggagcgtgt ccatgcggat ggcgtcgaag ccgacctgcc cgagccacca gagcgtgttc
     3181 tggatgaggt agcggcgcac ctcctcgtcg tcctgattga ggtcgggcag gatgtcgatg
     3241 aaccagcctt cgaggttgcg ccgctgcgtc tggcgcgtgg cgttcgggtc catcgtcgtc
     3301 cacttctgcc agttgttcga gaggtggttc tcgaccgtgc cgtagaacca cgtcggcgtc
     3361 gggcgctcgg tcgcccacgg gtggtaagga ctcgtgtggt tggcgatctg gtcctgaatc
     3421 accttgaggc cggcggcgtg cgccttgtcc accagctcgc gcagcttctt catgtcgccg
     3481 tagtgctctt cgacgccgta gaagtcgatg gcgccgtagc cgtggtaggc ggtcgtcggt
     3541 cgcttcgggc cgccctcgac ttccgggtac atctcgcgct cgtcgaggcg gtctgtgttg
     3601 tcgtagacgg ggttgatcca gagcgcggtg acgccgaggt ctttgaggta gggcaggcgg
     3661 tcgatgatgc cctgaaagtc gccgccgtgg tagtagcgcc ccttcgtgcg gtcgagcagg
     3721 ccgggcgact ttgcggggtt gttgttcgag gggtcgccgt cggcgaagcg gtcgggcatg
     3781 atgaagtaga tcacgtcgtc ggtggtgaag ccctgatagc ggcccgcgcg cgggatcggc
     3841 ggcactatct cgaacggcgc ttcgaccgcg cccgccgccg tcaccacgcg caggcggcga
     3901 gtcccggcgg cggccctctc gtcaatcgtc acgtccacga aaaggtaggt gccgcgctcg
     3961 ttgacgcgca cgaggccggt gccgatccct gcgccgaccg gctcgacgcg cgcgccgtgc
     4021 aggttccgac cgcgcagcat cacgcgcacc ggattgatcg agtggcccgc ccaccacgaa
     4081 ggcggctcga ccttcgagac ggcagacgcg ttttgcgccg cgcacgtgac cgcgcaaagg
     4141 atgacgagaa agccgctgac cgaaacgaga cgcgccgtgt gcttcatgat gcgtcggcct
     4201 cccccgcgtg gtgttcgtga tgcgcggtcg gttcgggctg cgggtcgcgc ccgtagagac
     4261 gtgtgaagta ttgaagtcgc gcggcgatct gcggcgtcag catctcgggg cggaagcgcc
     4321 agtcccagtt gccggaggtg ctggcgggca ggttcatgcg cgcctccgtg ccgaggccga
     4381 gcacgtcttg cagcggcatc aggcaggtgt cggagaccga ggcgagcgct gcccggatga
     4441 aggcccagtg aatctccgcg ccgtcggtgg cgaggtagct caggcagaag tcgcgctcgc
     4501 gctcgatctg ctcggcgctg cgcgtggagc ctgtgcccgc ctggctgttg aaccagccga
     4561 cgatggtgtc gttgtcgtgc gtgccggtgt agacgacgga gttcggcacg tagttgtggg
     4621 gcaggtcttt gttgcgcgcg tcggagctga agccgaactg gaggatgcgc atgccgggga
     4681 agccgaagtc gtcgcgcagc gcctccacgt cgggcgtgat gacgccgagg tcttcggcga
     4741 cgatgggcaa cacgccgagc gcgtcgcgga tcgcctgaaa cagttccctg cccggcgccg
     4801 gaacccagcg cccgcgctcg gcggtctggt cgccgcccgg cacttcccag taggccgcga
     4861 agccgcggaa gtgatcgatg cgcaggatgt ccacgagctt gagcgtcgcg ctcgcgcgct
     4921 cgatccacca gccgaagccg tcggcgcgca tgtgctccca gtcgtacagg gggttgcccc
     4981 aaagctgtcc ggtcttgctg aagtagtcgg gcggcacgcc tgcgacgacg cgcgggctgc
     5041 cgtccgcgtt gaggtggaac tgttcgggct gcgcccacac gtccgaagag tcgtgcgcga
     5101 cgaagatggg gatgtcgccg acgatgcgga tgcctttgtc gttggcgtaa gctttgagcc
     5161 gcgaccactg acggaagaag aggaactggt aaaacttgtg cgcgtcgatc tcgtcgcgca
     5221 agccctcgcg cgcggcggct aatgcttcgg gcaggcgccg caccatcggc gcctcccact
     5281 ggttccacgc cgcgccgc
//
LOCUS       DBNGOH010000358         2261 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4453331, whole genome shotgun sequence.
ACCESSION   DBNGOH010000358 DBNGOH010000000
VERSION     DBNGOH010000358.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2261)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2261)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2261
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4453331"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1058
                     /locus_tag="AC159P_07060"
     CDS             <1..1058
                     /locus_tag="AC159P_07060"
                     /inference="COORDINATES: protein motif:HMM:NF013646.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HIR3695249.1"
                     /translation="PVRRALGLGFEGSTFERMFYVADAQIDWHLSHDALHVCLARESF
                     VVFFPLKGERRYRIVGVFPEEFAKEEGDVLYEEIEQRIKDKATLELDIHDVEWFSTYK
                     VHTRHVSSFSAGRCFLAGDAAHIHSPAGAQGMNTGIQDAYNLAWKMALVLKGDADERL
                     LDTYNEERLENAKNLLQTTDRMFQLAAGPEWFLAFIRTNILPPVAKYILGLDTVKGFI
                     FPIVSQIGINYRHGSLSRHAGDGEFAVKAGDRMPYFQVDGESVYDRLRQPKFHWLTFA
                     GGRDDSQASGVELESRYAALVDFHAVPLDAQAAEAFGRREPFGVLLRPDNYIGFISAG
                     TAPGGVGSYLGEVIGRR"
     gene            complement(1199..>2261)
                     /locus_tag="AC159P_07065"
     CDS             complement(1199..>2261)
                     /locus_tag="AC159P_07065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HIR3695250.1"
                     /translation="VAAAPVPAQALTGSVQFFVGGTSVGTAPLINGVAELPLTVSSAA
                     GTYDVTAQFTSTNPAFYTDSSGGPSALTVSKEKASAAYTGDAALMTAGPNVSTATVRL
                     GARLTPEADGANFAGDITKAAVTFELFKSNNSGATPDLVVAGVPVDANGDAQALVGSL
                     AADLYLVNVKVDGANRFWTANPVGLGVLNIVVPSDEQRSTGGGWVAAAASRDGKANFG
                     FSVTSGNKAGQAKGNFTFTLGGADGFNYVLKSNSWQDGYLQFAAEPGTTPPVYARSNF
                     KGRGVVQKIDPLTGLVVASTGNYTFEVFTADGDGLTPKKPDALSITVWDSTGQVWHQV
                     GNSATLVTLGGGNITNKAK"
BASE COUNT          380 a          711 c          766 g          404 t
ORIGIN      
        1 gtcccgtccg gcgcgcgctc ggactcgggt tcgagggcag cacgttcgag cggatgttct
       61 acgtcgccga cgcgcagatc gattggcatc tcagccacga cgcgctgcac gtctgtctgg
      121 cgagagagtc gttcgtcgtc ttcttcccgc tcaaaggcga aaggcgttac cgcatagtcg
      181 gcgtcttccc ggaagagttc gcgaaggaag agggcgacgt tctgtacgaa gagatcgagc
      241 agcgcatcaa ggataaggcg acgctcgaac tcgacattca cgatgtcgag tggttttcga
      301 cctacaaagt ccacacgcgc cacgtcagca gcttctcggc gggcaggtgt tttctcgcag
      361 gggacgcggc gcacatccac tcgcccgccg gggcgcaggg catgaatacg gggattcagg
      421 acgcctacaa cctcgcttgg aagatggcgc tggtcttgaa aggcgacgcc gacgaaaggc
      481 tgctggacac atacaacgag gagcggctcg aaaacgccaa gaaccttttg cagacgaccg
      541 accggatgtt ccaactggcc gccgggccgg aatggtttct cgcgttcatc cgcacgaaca
      601 ttttgccgcc cgtggcgaaa tacatcctcg gcctcgacac ggtgaagggt ttcatcttcc
      661 ctatcgtttc gcagatcggc atcaactacc ggcacggctc actcagccgg cacgcgggcg
      721 acggcgagtt cgcggtcaaa gccggagacc ggatgcccta ttttcaggtg gacggcgaga
      781 gcgtttacga caggctgcgc cagccgaagt ttcactggct tactttcgcg ggcgggcggg
      841 acgactccca agcgtcgggg gtcgaactcg aaagccggta cgccgcgctg gttgacttcc
      901 acgccgtccc gctcgatgcc caagcggcgg aagccttcgg caggcgcgag cctttcggcg
      961 tgctgctgag gccggacaac tacatcgggt tcatctcggc gggaaccgcg ccgggcggag
     1021 tgggaagtta tctgggcgag gtcatcggtc gccggtaaaa tcaggcggcg tctttctacg
     1081 agcggatggt cgatgtcgtc ataaccgaag cgcgcaccgc aagcggaagg agaaagaggc
     1141 ttgagaccga acgtctcaag cctctttctt ttattgctga cggcttaccg cccgccgctc
     1201 actttgcctt gttcgtgatg ttgccgccgc cgagcgtgac gagggtggcg ctgttgccga
     1261 cctggtgcca gacctggccg gtgctgtccc agaccgtgat ggagagcgcg tcgggcttct
     1321 tcggggtcag cccgtcgccg tcggcggtga agacctcgaa ggtgtagttg cccgtggagg
     1381 cgacgacgag gccggtcaag gggtctatct tctggacgac gccgcggccc ttgaagttcg
     1441 agcgcgcgta gacgggcggc gtcgtgcccg gttcggcggc gaactggagg tagccgtcct
     1501 gccacgagtt gctcttgagg acgtagttga agccatccgc gccgcccagc gtgaacgtga
     1561 agttgccctt cgcctgcccg gccttgttgc cggacgtgac ggagaagccg aagttcgctt
     1621 tgccgtcgcg gctcgcggcg gcggcgaccc agccgccgcc cgtgctacgc tgctcgtccg
     1681 aaggcacgac gatgttcagc acgccgaggc cgaccgggtt cgccgtccag aaccggttcg
     1741 cgccgtccac cttgacgttg acgaggtata agtccgccgc gaggctgccc acgagcgcct
     1801 gcgcgtcgcc gttcgcgtcc acggggacgc ccgccacgac gaggtcgggc gtcgcgcccg
     1861 agttgttcga cttgaacagc tcgaaggtga cggcggcctt cgtgatatcg ccggcaaagt
     1921 tcgcgccgtc ggcctccggt gtcaggcgcg cgccgaggcg cacggtggcc gtcgagacgt
     1981 tcggccccgc ggtcatcagc gccgcgtcgc cggtgtaggc ggcggacgcc ttctccttac
     2041 taactgtgag cgcggacggg ccgccgctgc tatcggtgta gaacgcgggg ttcgtgctcg
     2101 tgaactgcgc cgtcacgtcg tacgtgcccg ccgccgagct gacggtgagc ggcagctccg
     2161 cgaccccgtt gataagcggc gcggtgccga ccgaagtgcc gcccacgaag aattgcacac
     2221 tgcccgtcaa cgcctgtgcg gggactgggg cggcggcgac c
//
LOCUS       DBNGOH010000359         1791 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3720401, whole genome shotgun sequence.
ACCESSION   DBNGOH010000359 DBNGOH010000000
VERSION     DBNGOH010000359.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1791)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1791)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1791
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3720401"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(51..1604)
                     /locus_tag="AC159P_07070"
     CDS             complement(51..1604)
                     /locus_tag="AC159P_07070"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019991466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase family protein"
                     /protein_id="HIR3695251.1"
                     /translation="MLATALCLALACPAAWGTQQRQQRQRAAAPHARRSRTPSLRLAR
                     LADRQPRNVIFILTDDQRYDALGFLRGQPFLETPHMDALARRGAHLPNAFVTTALCSP
                     SRASILTGRYAHRHRVVDNDSPIPPGLVFFPQYLQRAGYETAFVGKWHMGGPLDDPRP
                     GFDHWVSFKGQGSYLPNADGLNVNGRRVPQKGYITDELNTYALDWLKGRRGDKPFMLY
                     LSHKAVHAEFIPAERHRGRYAGKQFVPPLTMNPENVRGAPAWVRNQRNSWHGVDFPYH
                     GELDIAEYYRRYAETLLAVDEGLGQIVELLRQKGTLDSTLIFLMGDNGFAFGEHGLID
                     KRTAYEESMRVPLLVQCPELFRGGTVIDEVVSNIDIAPTILEAAGLRAPGDMDGRSFL
                     PLVEGRRGAWRDRLLYEYYWEWAFPHTPTMFALRGDRYKYIHYYGLWDINELYDVKAD
                     PKETRNLIGSPAHQETVRELRRQMFELLEKTDGLSIPVKPAQWGQQNLRREGGPKAAE
                     FPPELIKKP"
BASE COUNT          266 a          578 c          629 g          318 t
ORIGIN      
        1 gcggcggcgc ggcgaccacg gcgcggcggc tggcctgggc gataatctgt tcatggcttt
       61 ttaatcagct cgggcgggaa ctcggcggcc ttcggcccgc cctcgcgtcg caaattctgc
      121 tgcccccact gcgccggttt gaccgggatg gacagcccgt ccgtcttctc cagcagctcg
      181 aacatctgcc gccgcagctc gcggacggtc tcctgatgcg cggggctgcc gatgaggttc
      241 cgcgtctctt tcgggtcggc cttcacgtcg tacagctcgt tgatgtccca gaggccgtag
      301 tagtggatgt acttgtagcg gtcgccccgc agcgcgaaca tggtgggcgt gtgcgggaag
      361 gcccactccc agtaatactc gtagagcagg cggtcgcgcc aagcgccccg gcggccttcg
      421 accagcggca gaaaactccg cccgtccatg tcgcccggcg cgcgcaggcc cgccgcttcg
      481 aggatggtcg gggcgatgtc gatgttggag acgacctcgt cgatcaccgt cccgccgcgg
      541 aacagctcgg ggcactgcac cagcagcggc acgcgcatcg actcctcgta ggccgtgcgc
      601 ttgtcgatca gcccgtgctc gccgaaggcg aagccgttgt cgcccatcag gaagatgagc
      661 gtggagtcga gcgtgccctt ctgcctgagc agctcgacga tctggccgag gccctcgtcc
      721 acggcgagca gcgtctcggc gtaacgccgg taatactcgg cgatgtccag ctcgccgtgg
      781 taggggaagt caacgccgtg ccagctgttg cgctggttcc gcacccacgc gggcgcgccc
      841 cgcacgttct ccgggttcat cgtcagcggc ggcacgaact gcttacccgc gtagcgcccg
      901 cggtgtctct ccgcagggat gaactcggcg tgcacggcct tgtgcgagag gtagagcatg
      961 aacggtttgt cgccgcgcct acccttcagc cagtcgagcg cgtaagtgtt cagctcgtcg
     1021 gtgatgtagc ccttctgcgg gacgcgtctg ccgttgacgt tgaggccgtc cgcgttcggc
     1081 aggtagcttc cttgaccctt gaagctgacc cagtggtcga agccgggccg cgggtcgtcg
     1141 agggggccgc ccatgtgcca cttgccgacg aaggccgtct cgtagccggc gcgttgcagg
     1201 tattgcggga agaagacgag gccgggcggg atggggctgt cgttatcgac gacgcggtgg
     1261 cggtgcgcgt agcggcccgt gaggatcgag gcgcgcgagg gcgagcacag cgccgtcgtg
     1321 acgaaggcgt tcggcaggtg cgcgccgcgg cgcgcgagcg cgtccatgtg cggtgtttcg
     1381 aggaacggct gcccgcgcag gaaaccgagc gcgtcgtaac gctggtcgtc cgtgaggatg
     1441 aagatcacgt tgcgcggctg gcggtcggcg agtctcgcca gccgcagcga gggcgtgcga
     1501 cttcgccggg cgtgcggcgc ggcggcccgc tgccgctgct gccgctgctg cgtgccccac
     1561 gccgcggggc aggcgagcgc gaggcacaac gccgttgcta acaatctccg cgatgtgaaa
     1621 tttctttcct gcataattct tacgcgcgcg ggaaagcttt ggtcactcaa ggtattggga
     1681 gacgatagtg cgccacagcg agtagcctgc ctggctcatg tgcaggcgat cctcgacgaa
     1741 catctcaggg tacggtcgcc cgtccgctcc cagcatcggc gtgaagacat c
//
LOCUS       DBNGOH010000360         1820 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_54623, whole genome shotgun sequence.
ACCESSION   DBNGOH010000360 DBNGOH010000000
VERSION     DBNGOH010000360.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1820)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1820)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1820
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_54623"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            9..863
                     /gene="nadC"
                     /locus_tag="AC159P_07075"
     CDS             9..863
                     /gene="nadC"
                     /locus_tag="AC159P_07075"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014098605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HIR3695252.1"
                     /translation="MNWLDEGALFSQIGSFLQEDLGRGDITTQSTVLRQQRARGRFIA
                     KEGMTIAGLEAAVAVFSTLDSQQQIEAFVSDGDEVEEGKVIARTSGFADVLLGGERLA
                     HNLLQRLSGIATQTRRFVREVEGTKAQIVDTRKTTPGLRMLEKYAVLAGGGRNHRFGL
                     DDGVLIKDNHIALAGGVATAVERARQAVGHLHKVEVEVSSEAALREAIKSGADILLLD
                     NQTPEQTARLVEVARELAPHVMLESSGGISIENVRAYAEAGVDLISVGALTHSARAMD
                     ISFKIQPA"
     gene            935..1783
                     /locus_tag="AC159P_07080"
     CDS             935..1783
                     /locus_tag="AC159P_07080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695253.1"
                     /translation="MRRFSKGRTAAALAALLLLAAGLGEVHSQQRRTRPSRRAANPTA
                     RTTTPPTPTPTVSSSEPKLISTADDEAQEETPRRTARTTTRRTASQEAEEQQRERQQQ
                     EQLRSTVNQLSTQVERISEDLTEMKTDQRVLFELERLTRAEQRAESLRAQLRDVTDKE
                     FQHQERLAQIEDELQPDAIQRRAAVYGTINPSALREQIARGLERERERVRKQLELTTN
                     SRVRLEAAVASADQEVEKLRQRVEAADQQRLNPGPTSNTTGATTQPIITPATPQPTPP
                     PAEPPQ"
BASE COUNT          353 a          637 c          623 g          207 t
ORIGIN      
        1 aagctatcat gaactggctc gacgaaggcg cactcttttc gcagatcggc tcgttcctgc
       61 aagaggactt ggggcgcggc gacatcacga cgcagagcac cgtgctccgg cagcagcgcg
      121 cgcgcgggcg cttcatcgcc aaagagggga tgaccatcgc cggactcgaa gccgcggtgg
      181 ccgtcttctc gacgctcgac tcgcagcagc agatcgaagc cttcgtctcg gacggcgacg
      241 aggtcgaaga gggcaaggtc atcgcgcgca cctcgggctt cgccgacgtg ctcctcggcg
      301 gcgagcggct cgcgcacaac ctcttgcagc ggctctcagg gatagctacg cagacgcgcc
      361 gcttcgtccg cgaggtcgag gggacgaagg cgcagatcgt ggacacgcgc aagacgacgc
      421 cgggcctgcg gatgctcgaa aagtatgcgg tgctcgcggg gggcgggcgc aaccaccgct
      481 tcggtctgga cgacggcgtg ctcatcaagg acaaccacat cgcgctcgcg ggcggcgtgg
      541 cgacagcggt cgagcgcgcg cggcaggccg tcgggcacct gcacaaggtc gaggtcgagg
      601 tgtcgagcga ggcggccctg cgcgaggcga tcaagagcgg cgcggacatc ctcctgctgg
      661 acaaccagac gccggagcag acggcgcggc tcgtcgaggt ggcgcgcgag ctggcgccgc
      721 acgtcatgct cgaatcgtcg ggcggcatca gcatcgagaa cgtgcgcgcc tacgccgagg
      781 cgggcgtcga tctcatcagc gtcggtgcgc tgacgcactc ggcgcgcgcg atggacatca
      841 gcttcaagat tcagccggcg tgagcggcgg ccagcggaac aaaattccgg gcgcggcgtg
      901 taagtgatct atgccaaacg cgggggagat gacgatgaga aggttttcga agggacggac
      961 ggcggcggcg ctggccgcgc tgctcttgct ggccgcgggc ctcggcgagg ttcactctca
     1021 acagcggcgc acgcgcccct cgcgccgggc ggccaacccc acggcgcgca cgacgacgcc
     1081 gccgaccccg acgccgaccg tctcctcttc ggagccgaag ctcatcagca ccgccgacga
     1141 cgaggcgcag gaagagacgc cgcgccgcac cgcgcgcacg acgacgcgcc gcacggcgag
     1201 ccaggaggcc gaagagcagc agcgcgaacg gcagcagcag gagcaactgc gcagcaccgt
     1261 caaccagctc tccacgcagg tcgagcgcat ctccgaggac ttgacagaga tgaagaccga
     1321 ccagcgcgtg ctcttcgagc ttgagcgcct gacgcgcgcc gagcagcgcg ccgagagcct
     1381 gcgcgcgcaa ctgcgcgacg tgaccgacaa ggagttccag caccaggagc gactcgcgca
     1441 gatcgaggac gagctacagc ccgacgccat ccagcgccgc gccgccgtct acggcacgat
     1501 caacccctcg gccctgcgcg agcagatcgc gcgcggcctc gagcgcgagc gcgaacgcgt
     1561 ccgcaagcag ctcgaactga cgaccaacag ccgcgtccgc ctcgaagcgg cggtcgcctc
     1621 cgccgatcag gaggtcgaga aattgcgcca gcgcgtcgag gccgccgacc agcagcgcct
     1681 caaccccggc cccacttcga acaccaccgg cgcgaccacg caacccatca tcacgcccgc
     1741 gacgccgcag cccacgccgc cgcccgccga gccgccgcaa tgaaagtcag aagtcagaac
     1801 gcagaaggat gaataaaagc
//
LOCUS       DBNGOH010000361         4532 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_787470, whole genome shotgun sequence.
ACCESSION   DBNGOH010000361 DBNGOH010000000
VERSION     DBNGOH010000361.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4532)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4532)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4532
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_787470"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..552
                     /locus_tag="AC159P_07085"
     CDS             <1..552
                     /locus_tag="AC159P_07085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695254.1"
                     /translation="QPDFIIHHVEIRHDAQGRGTISFERKQDAEAITDPLEVSADALA
                     RIKGLYDALRFLDSDVNYQGKREYPSYGKTVLRLTSGGRERAAEFNYPDDPNAMALMQ
                     EYRRLTEQAVFVFEIKLAVEMQPLEAPKLMNKLETLLRLNQISDPKQLTPLIRTLSTD
                     ERLPLIARNHAVRLLKKLEKEKQ"
     gene            complement(709..834)
                     /locus_tag="AC159P_07090"
     CDS             complement(709..834)
                     /locus_tag="AC159P_07090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695255.1"
                     /translation="MNDAASRELLVILSTMAALFVFGLVAVLIFIRTWRKEKGKQ"
     gene            complement(831..2177)
                     /locus_tag="AC159P_07095"
     CDS             complement(831..2177)
                     /locus_tag="AC159P_07095"
                     /inference="COORDINATES: protein motif:HMM:NF013741.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HIR3695256.1"
                     /translation="MLIALKLLAVLLLVIANGFFVAAEFAFVGVRRSRIEALAEAGDR
                     AAQRLLAILNNLNVYLSAAQLGITLASLGLGYLGEPAVAHLIEGPLMRAGVPESAVHI
                     IAFIIGFTIITCLHIVIGEQAPKLLGLERAEKVAMASALPMNAFAWVAYWPVRALDWA
                     SARTVRLFGLHATTDHASIYTEEEIRQLIEMSRKGGHIEEEEQTLINRVFDFSEAEVR
                     EAMVPRTVVMALPVTATLAEAEAAFCEHGFSRLPVYRERLDDIVGVLFMKDLMPCLRT
                     VSPVGFDIEKMLHPPLFVPSTAKLGSVLAQMQAAQTHLACVVDEHGGLEGIVTLEDLL
                     EEIVGEINDEYDEESRSQIVEEPDGTYLLDGMLAVRDANRRFNLKLPEEGGYTTMAGF
                     LLDKAGRLLKPGESVEHEGARFRVEHVDRRRIRRIRYAPAPAVKTEEPEQSEAAPA"
     gene            complement(2247..3380)
                     /locus_tag="AC159P_07100"
     CDS             complement(2247..3380)
                     /locus_tag="AC159P_07100"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008126 - acetylesterase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YheT family hydrolase"
                     /protein_id="HIR3695257.1"
                     /translation="MENVTDRAHEIERGATVPLIPPQSLSAPLREMARLVRSNPFEPH
                     PLFRGGHAQTVLAALRLPRYAELRRDEPGEWESRLLEVEPGARVLVKCRWQAERRAAP
                     TLLLLHGLEGSSESLYVRGTARKAFGAGFNVVRMNMRTCGGTEHLTPTLYHSGMSADI
                     HHVFRELARGEGLTRVFLAGYSMSGNMVLRLAGDSGAEAPPELAGVCAVSPSLDLAAC
                     ALAIERRANRLYQWSFIRSLRRRLRRKRRLHPELYDTRGLRHVRTIRQFDERFTAPHG
                     GYRDADDYYERTSSISVIHRIRVPTLVLHAEDDPIVPPEAFRDRAFKENPHVLLVLTP
                     RGGHTAFIARAAEGEDRRWAENRVVDFCRLLCRCGLADAGQEK"
     gene            complement(3576..3782)
                     /locus_tag="AC159P_07105"
     CDS             complement(3576..3782)
                     /locus_tag="AC159P_07105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695258.1"
                     /translation="MSRNPNADPPQPGDQPELPPAEPQDPAQPQPIPLPPDANPSPVA
                     PVREPDAPPPPAGDPQPTEPPRLA"
     gene            3995..4396
                     /locus_tag="AC159P_07110"
     CDS             3995..4396
                     /locus_tag="AC159P_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695259.1"
                     /translation="MNTIVVTYLCYLCISVALTVWVAQTLHKNGRIFLVDVFHGNEAL
                     ADSVNHLLVVGFYLINFGYVTLALRIGYDVETARAGIEALSWKVGLVLIVLGTMHFFN
                     LYIFSRIRRRPGGTPRFQHESYKGFQDEAEA"
BASE COUNT          745 a         1440 c         1603 g          744 t
ORIGIN      
        1 cagccggact tcatcatcca ccacgtcgag atcaggcacg acgcgcaagg gcgcggcacc
       61 atcagcttcg agcgcaagca ggacgccgag gccatcaccg acccgctcga agtctcggcg
      121 gacgcgctcg cccgcatcaa ggggttgtac gacgcgctcc gcttcctcga ctcggacgtg
      181 aactatcagg gcaagcgtga gtacccgagc tacggcaaga ccgtcctgcg cctgacgagc
      241 ggcgggcgcg agcgcgcggc ggagttcaac tacccggacg acccgaacgc gatggcgctc
      301 atgcaggagt accgccgcct caccgagcag gccgtcttcg tcttcgagat caagctcgcc
      361 gtcgagatgc agccgctcga agcgccgaag ctgatgaaca agctcgaaac gctgctgcgt
      421 ctcaaccaaa tctccgaccc caagcaactc accccgctca tccgcacgct ctcgaccgac
      481 gagcggctcc cgctcatcgc acgcaaccac gcggtgcgcc tactgaagaa actggaaaaa
      541 gagaaacagt aggcggtagg cagcaggcag taggcagtca aaacagaagg gcgaagaggc
      601 ttgagaggta aagtcctgag cctctccgcg cttcaagcaa caaagcagga ggcttgagac
      661 gcaacggctc aagcctcttt ttactgccta ctgcccgctg cctactgcct actgcttccc
      721 cttctccttc cgccatgtgc ggatgaagat gaggacggcg acgaggccga agacgaagag
      781 ggcggccatc gtggagagga tgacgagcag ttcgcgcgag gcggcgtcgt tcatgcgggg
      841 gcggcctcgc tctgttccgg ctcctcggtc ttgacggcgg gcgcgggcgc gtagcggatg
      901 cggcggatgc ggcggcggtc tacgtgctcg acgcggaacc gcgcgccctc gtgctcgacc
      961 gactcgcccg gcttcagcag ccgcccggcc ttgtcgagca ggaacccggc catcgtcgtg
     1021 tagccgccct cctccgggag cttgaggttg aagcggcggt tggcgtcgcg caccgcgagc
     1081 atcccgtcca ggaggtaggt gccgtcaggc tcctcgacga tctgcgagcg ggattcttcg
     1141 tcgtactcgt cgttgatctc gccgacgatc tcttcgagta aatcctccag cgtgacgatg
     1201 ccttcgagtc cgccgtgctc gtccacgacg caggcgaggt gcgtctgcgc ggcctgcatc
     1261 tgcgcgagca cggagccgag cttggcggtc gaggggacga agagcggcgg gtggagcatc
     1321 ttctcgatgt cgaacccgac ggggctgacg gtgcgcaggc agggcatcag gtccttcatg
     1381 aagagcacgc cgacgatgtc gtccagccgc tcgcggtaga cgggcaggcg cgagaagccg
     1441 tgctcgcaga aggccgcctc ggcctccgcg agcgtggcgg tgacgggcag cgccatgacg
     1501 acggtgcgcg gcaccatcgc ctcgcgcacc tcggcctcgg agaagtcgaa gacgcggttg
     1561 atgagcgtct gctcctcctc ctcgatgtgc ccgcctttgc gcgacatctc gatgagctgg
     1621 cgaatctcct cctcggtgta gatcgaggcg tggtcggtcg tggcgtgcaa gccgaagagc
     1681 cggacagtgc gcgcgctggc ccagtcgagc gcgcgcacag gccagtaggc gacccacgcg
     1741 aaggcgttca tcggcagggc cgaggccatg gccaccttct ctgcgcgctc aagcccgagg
     1801 agcttcggcg cctgctcgcc gatcacgatg tgcaggcacg tgatgatggt gaagccgatg
     1861 atgaaggcga tgatgtggac ggcggactcg gggacgccgg cgcgcatgag cggcccttcg
     1921 atcaggtgtg cgacggcggg ttcgccgagg tagccgaggc cgagcgaggc gagcgtgatg
     1981 ccgagctgcg cggcggagag gtagacgttg aggttgttga gtatcgcgag caggcgctgc
     2041 gcggcgcggt cgccggcctc ggccagcgcc tcgatgcgcg agcggcggac gccgacgaag
     2101 gcgaactccg cggccacgaa gaagccgttg gctatcacta gcagaaggac ggctagcagt
     2161 ttgagcgcaa tgagcatcgt ccgttccggc gggccgaccg cgccccggcc cgttcaattg
     2221 ttcagactgg aaaatctcgc ctaagcttat ttctcttgtc ccgcatctgc aagcccgcag
     2281 cggcagagca ggcggcagaa gtccacgacg cggttctcgg cccagcggcg gtcttcgcct
     2341 tcggcggcgc gcgcgatgaa ggcggtgtgg ccgccgcgcg gcgtcagcac gagcagcacg
     2401 tgggggttct ccttgaaggc gcggtcgcgg aaggcttcgg gcgggacgat ggggtcgtcc
     2461 tcggcgtgga gcacgagggt ggggacgcgg atgcggtgga tgacggagat cgagctggtg
     2521 cgctcgtagt agtcgtcggc gtcgcggtag ccgccgtgcg gcgcggtgaa gcgctcgtcg
     2581 aactggcgga tggtgcgcac gtggcggagg ccccgcgtgt cgtagagttc ggggtgaagc
     2641 cgccgcttcc ggcggaggcg acggcgcagg ctccgtatga agctccactg gtagaggcgg
     2701 ttggcgcgcc gctcgatggc gagcgcgcac gcggccagat cgagcgacgg cgagacggcg
     2761 cacacgcccg cgagttcggg cggggcttcg gcgccggagt cgcccgcgag ccgcaagacc
     2821 atgttgccgc tcatcgagta gcccgcgagg aaaacgcgcg tcagcccctc gccgcgggcg
     2881 agttcgcgga agacgtggtg gatgtccgcg gacatgccgc tgtggtagag cgtcggggtc
     2941 aggtgctcgg tgccgccgca cgtccgcatg ttcatgcgga cgacgttgaa gcccgcgccg
     3001 aaggctttgc gcgccgtgcc ccgcacgtag agcgattcgc tcgaaccttc cagcccgtgg
     3061 aggagcagca gcgtcggcgc cgcgcggcgc tccgcctgcc agcggcactt cacgagcacg
     3121 cgcgcgcccg gctcgacttc cagcagccgc gactcccatt cgcccggctc gtcgcgccgc
     3181 agctccgcgt agcgcggcag gcgcagcgcg gcgagcacgg tctgcgcgtg gccgccgcgg
     3241 aagagcgggt gcggctcgaa cgggttcgag cggacgagcc gcgccatctc gcgcagcggc
     3301 gcgctcaaac tctgcggcgg gatgagaggc accgtggccc ccctctctat ctcgtgtgcc
     3361 ctgtcggtga cgttctccat cggcgtggtg tgacgcggta gcggcgtaca acctcacgtc
     3421 ggattataaa tgatgggcgc ggattgcgga acggtaagca gacggggaga cggggagaag
     3481 gggagtgttg gagacgtgta ggctcaacga ttttctctct ccccctctcc ccgtctccct
     3541 ttctcccttt ctccccttct ccccttcttg aagtcttacg ccagccgcgg cggctccgtg
     3601 ggctgcgggt ctccggcggg cggcggcggc gcgtcgggtt cgcgcacggg cgcgaccggg
     3661 ctggggttgg cgtcgggcgg cagggggatg ggctgcggct gcgcggggtc ttgcggctcg
     3721 gcgggcggca attcgggttg gtcgccgggc tgcggcgggt ctgcgttcgg gttgcggctc
     3781 atggcgtgat gtccctccgt tcgttatgca gaatgcgaag actctagcac gattccgcaa
     3841 tgcgccacac acgcgcgcgc cgattcatcg gacgaaagag cgcgtgtggc gcgtgcgcgt
     3901 gatggcacgg aggttgaagc ggatagtggc gagagaccac ccgccgcggg gcgcgtcgcc
     3961 cccgaagaga ggcgggccgg aaaaggagaa ggagatgaac accatcgtcg tcacatacct
     4021 ctgctatctg tgcatcagcg tcgcgctgac ggtgtgggtg gcgcagacgc tccacaagaa
     4081 cgggcgcatc ttcctcgtcg atgtcttcca cggcaacgag gcgctggccg actcggtgaa
     4141 ccacctgctg gtggtcggct tctacctcat caacttcggc tacgtgacgc tcgcgctccg
     4201 gatcggctac gacgtggaga cggcgcgcgc cgggatagag gcgctgtcgt ggaaggtcgg
     4261 cctcgtgctc atcgtgctcg gcacgatgca cttcttcaac ctctacatct tcagccgcat
     4321 ccgccgccgc cccggcggca cgccccgttt ccagcacgag tcctacaaag gttttcagga
     4381 cgaagccgaa gcgtgacgca ggcgaaaagg ttataagggg aaaaggggag gctgggaata
     4441 gtgttgtgcc tttccctctt cgccttttcc ccttttaaca ttttccccct tctgcgcttg
     4501 cgcgttggag gtcggcgacg acgaagcgga tc
//
LOCUS       DBNGOH010000362         5073 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2070931, whole genome shotgun sequence.
ACCESSION   DBNGOH010000362 DBNGOH010000000
VERSION     DBNGOH010000362.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5073)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5073)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5073
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2070931"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(41..1813)
                     /locus_tag="AC159P_07115"
     CDS             complement(41..1813)
                     /locus_tag="AC159P_07115"
                     /inference="COORDINATES: protein motif:HMM:NF012698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPR end-of-group domain-containing protein"
                     /protein_id="HIR3695260.1"
                     /translation="MDHQYRFGPFYVDTRERLLLREGEHITLKQKAVDVLLILLRESP
                     RIVEKEQFMSEVWRDAFVEEGSLTVSVSEIRKALGVGPEGRQYIETIPRRGYRFTPEP
                     WRSPLEAEGPPATVRASAAEAPPARSTARAVAVLPFANLSSDGALDYLADGFADSIIN
                     SLSRLSGLRVKASGSSFRYRGIDVDCQLVGRDLGVGAVVSGRILQLRDRLVVRVALVD
                     ATDGAQLWGEQYDRKPSDLLEVQGEIAGEVAGQLQLRLTGEEQHRLAKLSTESVEAYR
                     AYLKGRSFWSKRPQPGFLKGIEYFRRAIEFDPGYALAYSGLADAYGVLGSWEVGMLPP
                     REAAAKARAAAIRALEIDDQLAEAHTSLAHINLHYDWDWTVAEGAFKRALELSPSYVH
                     AHHWYSHLCVATGRTEQGYAAGLRALELDPLDLIINVHMAWHHWLTREPALALEQCAR
                     GRELEPASPWWHLFGGLAYEQKEMYAEAIEELRRAAELSDATKIALAGLGHAAGLALE
                     RDEAERVLSELERLSGSKYVPAYDRAVAHLGLGDTGKALEYLTRACDERSSWMPYLNV
                     EPRLDPLRSDPRFQELVRRVGLPQ"
     gene            2025..3299
                     /locus_tag="AC159P_07120"
     CDS             2025..3299
                     /locus_tag="AC159P_07120"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0140905 - haloperoxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vanadium-dependent haloperoxidase"
                     /protein_id="HIR3695261.1"
                     /translation="MFEDNQRATTLQPYRRRPAPYVVAALLLTLGLAGPTKADVVTDW
                     NATVTTVSAFMEKRAAAVAIVDAAYMHAAIYDAVNSIDRRFTPYAVSPASPPPAGTSP
                     EAAAVAAAYRVLVHMFPNQRAFLDERYAQSLALIPDGQARADGIALGAEIAGKFIELR
                     NGDGWNASVAYTPGIGPGAWQPTGGAPVAPWLAKMRPFAILTPSQFRAAGPPALGSAQ
                     WAQDFNETKLFGALDNSARTPEQTEIGRFYTEHTGLQYARIFRDFAAQQKLSLADEAR
                     LFAALYVTGADALIAGFDSKYYFGFWRPVTAIRAGATDGSELTAGDPDWAPLAATPGH
                     PEYPAAHGCLTAAWAEVLKNFFGTKRVHITLTSAVTGTSRTFDNTDDLIKEIIDARIF
                     GGMHYRTSGVHGAVIGKKVARFVADHYFHPVK"
     gene            complement(3736..3936)
                     /locus_tag="AC159P_07125"
     CDS             complement(3736..3936)
                     /locus_tag="AC159P_07125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695262.1"
                     /translation="MVNRTDTPLMSFYRKKKREKGAGKAICATARKLLTIIFVLLKKE
                     LDYWYLEDRLYNRKLRALNAAA"
     gene            complement(3909..4253)
                     /locus_tag="AC159P_07130"
     CDS             complement(3909..4253)
                     /locus_tag="AC159P_07130"
                     /inference="COORDINATES: protein motif:HMM:NF014430.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HIR3695263.1"
                     /translation="MLRAALRQIAQLDAEVRKIEEEAARRGQTLKGLRRLLQVHGLNL
                     LSAISLLAEVGDIALFHTSKRLVSYAGLATSTRQSSETTRHGGITKRGGSGCGRSAFR
                     RCWRWSTGRTRR"
     gene            complement(4196..4777)
                     /locus_tag="AC159P_07135"
     CDS             complement(4196..4777)
                     /locus_tag="AC159P_07135"
                     /inference="COORDINATES: protein motif:HMM:NF013698.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HIR3695264.1"
                     /translation="MRHIGVDLHKTNFVACFLAADDTRRLETYPLTRDGLARFKRQLR
                     KVDEVAVEATQNAHYFYDQVKGHGGRVAVVDTYRFGVIARSKKKTDKADAAALARFLK
                     LGWLSEVPVPSEQVRCLRQLLQARETLVSLRTKLKNMAHAAFSRNGIALTRAAFASAV
                     SRARLARRDDLPTCSCCAPRCGRSRSSTRRSGR"
BASE COUNT          836 a         1811 c         1593 g          833 t
ORIGIN      
        1 taggacatgc ggccgcctgc ggctaattaa attatgccca ctactgcggc aacccgacgc
       61 gacgcacaag ttcttggaag cgcgggtctg agcgcagtgg gtcaagcctc ggctcgacgt
      121 tgaggtaggg catccagctg gaacgctcat cgcacgccct ggtgaggtac tccaaagcct
      181 tccccgtgtc gccgaggccc agatgagcca ccgccctgtc gtaagcgggt acgtatttac
      241 tgcccgacaa gcgctccagc tcgctgagca ccctctccgc ctcatcccgc tccagcgcca
      301 agccagcggc atgccccagc ccagccagcg cgatcttggt ggcatcggat agctccgcgg
      361 cccgccgcag ctcctctatg gcttccgcgt acatctcctt ctgttcgtaa gccagcccgc
      421 cgaagagatg ccaccacggt gaggccggct ccagctctcg tcccctcgcg cactgctcca
      481 gcgccaacgc cggctcgcgc gtcagccagt gatgccaagc catgtggacg ttgatgatga
      541 ggtccagcgg gtcgagctcc agcgcgcgca ggcccgcggc gtacccctgc tcggtccgcc
      601 ccgtggccac gcacaggtgc gagtaccaat gatgcgcgtg aacataactc ggactcaact
      661 ccagggcgcg cttgaacgcg ccctccgcga ccgtccagtc ccagtcatag tgcaggttga
      721 tatgggcgag cgacgtgtgc gcctcagcca gttggtcgtc tatctccaag gccctgatgg
      781 cggccgccct ggccttcgcc gccgcctccc gcgggggcag catcccgacc tcccatgagc
      841 cgagcacccc gtaggcgtcg gcgaggccgg agtacgcgag ggcgtaaccc gggtcgaact
      901 ctatcgcgcg gcggaagtac tcgatcccct tcaagaaccc gggctgcggc ctcttgctcc
      961 agaaggagcg ccccttcagg taagcgcggt acgcctcgac gctctccgtg gagagtttgg
     1021 ccaggcggtg ctgctcctcg cccgtcagcc gcagttgcag ctgtcccgcc acctcgcccg
     1081 ctatctcccc ctgcacctcc agcaagtctg acggcttgcg gtcgtactgt tccccccaca
     1141 gttgcgcccc gtcggtcgcg tcgacgagcg ccacgcgcac gaccagccgg tcccgtaact
     1201 ggaggatccg cccgctgacg accgcaccca cgcccaggtc gcggccgacc aactggcagt
     1261 cgacgtcgat gccgcgatag cgaaagctgc tcccgctggc cttcacccgc aacccgctta
     1321 gccgcgagag gctgttgatg atgctgtcgg cgaacccgtc agccaagtag tcgagcgcgc
     1381 catccgagct caggttggcg aaggggagga cggcgaccgc gcgcgcggtc gagcgcgccg
     1441 ggggcgcttc ggcggccgag gcacgaaccg tggcgggcgg gccctctgcc tccagcgggc
     1501 tccgccacgg ctcgggcgtg aagcggtaac cccgccgcgg gatggtctcg atatactgcc
     1561 gcccctcggg gcctactccc agcgccttcc tgatttcgga gacgctgacg gtgaggctgc
     1621 cttcctcgac gaaggcgtcg cgccagacct cagacatgaa ctgctccttc tcgacgatac
     1681 gcgggctctc ccgcagcagg atgagaagga cgtccaccgc cttttgcttg agcgtgatgt
     1741 gctctccctc gcggagcagc aggcgctcgc gcgtgtcgac gtagaacggg ccgaagcgat
     1801 actggtgatc cactcttcgc caacccttcc gttaagaact cggtaagaaa caaataagaa
     1861 cgggcgaagg acttacccgc acgagttcgc ctacctttcg cgtgcggacg gcggagggtg
     1921 ccccgacggc gcacattcta gtggccgcgc ccggcgctgg caacgccctg cggggcgcac
     1981 gacccgttaa cttaaacgat aaacccggaa ggagccaaca cggcatgttc gaagataacc
     2041 agagggctac cacccttcaa ccgtaccggc ggcgcccggc gccttacgtc gtcgcggcgc
     2101 tgctgctcac cctcgggctg gcaggcccga ccaaggccga cgtggtgact gactggaacg
     2161 cgaccgtcac caccgtctcc gccttcatgg agaagagggc cgcggcggtc gccatcgtcg
     2221 acgcggccta catgcacgcc gccatctacg acgcggtcaa ctccatcgac cgccgcttca
     2281 ccccctacgc cgtttccccc gcgagcccgc cgcctgcggg cacttcgccc gaggccgccg
     2341 cggtcgcggc cgcctaccgc gtcctcgtcc acatgttccc caaccagcgg gctttcctcg
     2401 acgagaggta cgcccagtcg ctcgcgctga tcccggacgg gcaggccagg gcggacggca
     2461 tcgcgctcgg cgccgagatc gcaggtaagt ttatcgaact caggaacggc gacggctgga
     2521 acgcttctgt ggcctacacg ccggggatcg ggcccggcgc ctggcagccg accggcggcg
     2581 cccccgtggc gccgtggctg gcgaagatgc gccctttcgc gatcctgacc ccctcgcagt
     2641 tccgggccgc cgggccgccg gccctcggca gcgcccagtg ggcgcaggac ttcaacgaga
     2701 cgaagctctt cggagcgctc gacaactccg cgcgcacgcc cgagcagacc gagatcgggc
     2761 gcttctacac cgagcacacg ggcctgcagt acgcgcgcat cttccgcgac ttcgccgccc
     2821 agcagaagct gagcctggcg gacgaagccc gcctcttcgc cgcgctctac gtgacgggcg
     2881 ccgacgcgct catcgccggc tttgactcga aatactactt cggcttctgg cgcccggtga
     2941 ccgccatccg ggctggcgcc acggacggca gcgagctgac ggcgggcgac ccggactggg
     3001 cgccgctggc ggcgacgccc ggccaccccg aatacccggc ggcgcacggc tgcctcacgg
     3061 ccgcctgggc cgaggtgctc aaaaacttct tcggcaccaa gcgcgtccac atcacgctga
     3121 cgagcgccgt cacggggacg tcacgcacct tcgataacac cgacgacctg atcaaggaaa
     3181 tcatcgacgc ccggatcttc ggagggatgc actaccgcac ctccggcgtg cacggggcgg
     3241 tcatcggcaa gaaggtggcc cgctttgtgg ccgatcacta cttccacccg gtgaaataaa
     3301 ttcccccgca gggcgtccgg cctcatcagg gcgcgttccg ccgacacggc aggcgtatct
     3361 cggaggcgta tgggacggcc cggactcaag ttaaggcagc ccgggccgtc ccggcattcg
     3421 gaaggagtcg agctcgtgaa tataagcaaa ccggtgacgg acgcgctcga aaggcggctc
     3481 gaggagatgt ggaggtgggg tactacggcc acttcgcccg atacctcgag ccgagcgcgg
     3541 ctcgccaggt tcccctaaaa cgtcgggcgc ccattagcct gacttcagac ccttttccca
     3601 tcttcgtcgg gggctgggcg gtcgaggtca ccgtttgcgg tggggacggg tgacgagggg
     3661 gaggggtgtc aacggcgggc cggggtgttg ctatcggggc gccgggtgcg tgccgctcct
     3721 atgcaaagtc aacgttcaag ccgcggcatt gagcgcccgc agcttccggt tgtaaagtct
     3781 gtcctccagg taccagtagt cgagttcttt cttcagcagc acgaagatga tcgtcaacag
     3841 cttgcgcgcc gtcgcgcaga tggccttgcc cgcacccttc tcccgcttct tcttccgata
     3901 aaaactcatc agcggcgtgt ccgtccggtt gaccatcgcc agcaccgcct gaatgcagac
     3961 cgtccgcagc cgcttccgcc ccgcttcgtg atcccgccgt gccgcgtcgt ctcgctcgac
     4021 tgccgcgtcg aagtcgccag ccccgcgtag gagaccaacc gcttcgaggt gtggaagagc
     4081 gcgatgtcgc ccacctccgc cagcaggctg atggccgaga gcaggttcag cccgtgcacc
     4141 tgaagcagcc gccgcagacc cttgagagtc tggccgcgcc gcgccgcttc ctcctctatc
     4201 ttcctgacct ccgcgtcgag ctgcgcgatc tgccgcagcg cggcgcgcag cacgagcagg
     4261 tcggcaggtc gtcgcgacgg gccagccgtg cgcggctaac agcagaggcg aaggcggcac
     4321 gggtcagcgc gatcccgttg cgcgagaagg cggcgtgcgc catgttcttg agcttggtgc
     4381 gcagcgagac cagcgtctct ctggcctgaa gcagttgccg caagcaacgc acctgctccg
     4441 acggcacggg cacttcggat agccagccca gcttgaggaa gcgcgccagc gccgcggcgt
     4501 cagccttgtc cgtcttcttc ttcgacctgg cgatgacgcc gaagcggtag gtatccacca
     4561 ccgccacgcg gccgccgtgg cccttcacct gatcgtagaa gtagtgggcg ttctgcgtcg
     4621 cctcgaccgc cacctcgtct accttcctga gttgccgctt gaagcgggcg agcccgtcgc
     4681 gcgtgagcgg gtaagtctcc aggcgtcggg tgtcatccgc ggcgaggaag caggcgacga
     4741 agttcgtctt gtgcaggtca accccgatgt gtctcacggc ctcgcacctc cttcgggtca
     4801 aaaaggaggt cgaggcgcag cggagactgc tcggcgtagc tccacgctcc tatccgggat
     4861 cgggtgtccc actccgtggt gcgggcaggg gctcctcggg aagaccacac tgttagccgg
     4921 gctctcacgc ccacaacggc ctccggccct cgccccgtct ctcgctcgcc gcctccctgt
     4981 tggtggcacg agcatgctac accgaacgct ctgcttgtat tacgacctgc tatgagtgca
     5041 gtcaagcctc attctcgaaa gcgtagcgtt gaa
//
LOCUS       DBNGOH010000363         9236 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_422176, whole genome shotgun sequence.
ACCESSION   DBNGOH010000363 DBNGOH010000000
VERSION     DBNGOH010000363.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 9236)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9236)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9236
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_422176"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1190
                     /locus_tag="AC159P_07140"
     CDS             <1..1190
                     /locus_tag="AC159P_07140"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HIR3695265.1"
                     /translation="FTALSLNGAVTLGEGTDDSSGAAPREVRGKFQTRWGAGAHAVNL
                     FSGRGRIRLAALSREAQEAAARSHTPRPPRIDDPSGGERDAPVRRPTPQPAETPQEVD
                     EEEVVRVESDLVTLNISVVDRASGRGLAGLAQGDFRLAEDNVEQEIAHFEAADAPFDL
                     LLLIDLSGSTAKVTDIIRSAALRFVAATRPRDRVAVLTFAAGTDIVSPLTDDRRALRE
                     AISRMKPPTGDTRLYDALAGAMAYLERESAPTRRRAIVMMSDGLDSTMPNVTGVGSEL
                     PYAEVLDRAREFDGLLYTIWTSTEYEAFSPEDIQPETFDLAHDRTKEFADAGGGAFYL
                     VERMEDLAGAYERVVADLGTVYSLSYRPTNKQRDGSWRAIRVRLPRHAGAVARGKRGY
                     YAK"
     gene            1368..2726
                     /locus_tag="AC159P_07145"
     CDS             1368..2726
                     /locus_tag="AC159P_07145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695266.1"
                     /translation="MRTVGRGLIFYTAIVALVALVVVQFVACGDKGAEPAAAVVPTVA
                     ARRTLSPLPERALSPRLKAARERAMRAAAAARGLAWKGEVGMTELSGWEYGTRTKEIA
                     EVLGTEDLSGFSKLAVAGGMLPEGTDLASLAVTFTAATAGATYSPLDKQVILLAEDAK
                     RGAPANQSLLTHEFVHALQDQHFDLLKTLLARPFDFDRTEALFALVEGDAMNVERRIE
                     AGEKEWSRRTLEEIARHEDERFAPYRSQIGALFPPLLTETFIFRYRDGARFVEAMRRA
                     RPAVGVDDIFRRPPASSEQVLHPEKYLAGEAPREVDANAEGLREDGWEALASTPLGEI
                     GVRGLLLAGVADAEARKAAAGWGGDRAFVFSRAGRDSAQLFVWQTAWDGREDAREFFR
                     AYNTLLRRGRGAAQADGGEEAALWREGELSTLVRLEGDTVLVLRGAERDVAAALRIVT
                     RR"
     gene            2896..3948
                     /locus_tag="AC159P_07150"
     CDS             2896..3948
                     /locus_tag="AC159P_07150"
                     /inference="COORDINATES: protein motif:HMM:NF016648.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HIR3695267.1"
                     /translation="MRNTPRHTASCLLALVFASACAAVTAQGQTRDLRIVKAVAGGVN
                     FVSGDVKLQAPGRSGWQAITMKDDLKSGDIVKTGSDGRVEVLLNPGSYLRLGVNSEFE
                     LTDASLDKLRLKIYKGSAVVEATGYDDMLLSIAIDTPRTELRLVRRGIYRINVTPTGE
                     TEIAVQKGRALVGKESAATVVKGGKVARVDAGGVEVAKLDKKNLDALDLWSKERAEEL
                     AKLNRRIKAKQTNTLLANANFNRFYGRFGHGVWLYDPVSRCYTFLPFFGSWSSPYGSG
                     YGSYYPAYPCYYGGCHSSGRWNNDPWAGNMPSSPSPKSYDPPPSSSPPSFSLPVSRGS
                     GGGDSTAPSAPARSKP"
     gene            complement(4015..4188)
                     /locus_tag="AC159P_07155"
     CDS             complement(4015..4188)
                     /locus_tag="AC159P_07155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695268.1"
                     /translation="MVGGTGQALDAFGANAVTHRTTVANLFMVGDTVFPGNGVAAVTH
                     AALVVANEIAPPR"
     gene            complement(4336..5514)
                     /locus_tag="AC159P_07160"
     CDS             complement(4336..5514)
                     /locus_tag="AC159P_07160"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013739.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0016116 - carotenoid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HIR3695269.1"
                     /translation="MSYEVVIVGGGLGGLTAAALLSARGLDVCLLERGSQVGGCAAPF
                     ESAGHRFERGAGLYASWQPGGIHERVFAELPVAPPEVRRAAPSAYVVRLPDKTEVRVG
                     LAGDEHLAEVRAAFPECAGEAERFYVEAAEVAGALERAAARLPALATASKLQRVMLAA
                     REPLLAPRVLASLEHTAARHLAGTSARFRRFVDAQLQIFAQVPSERCAYPYAAVALSQ
                     PTRGMYAMRGGAGALAASLVESITKSGGTVRLNTTALRLSFDESGRATGVDLLSGETV
                     GATRAVVSNLTVWDTYGRLIGLGRSPAEARARVKRLRGWGAYQMFLSADEEALARLPT
                     EQILALDDWQEGREFDPERALLMFSAAPAWDARAPAGKRAVTVSTFTEAEPWFSFHED
                     "
     gene            complement(5519..6403)
                     /locus_tag="AC159P_07165"
     CDS             complement(5519..6403)
                     /locus_tag="AC159P_07165"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070007 - obsolete glutamic-type
                     endopeptidase activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HIR3695270.1"
                     /translation="MSTPITPIPQSTYASFEPPPPTVADEINPDNPPWGIPGGVGLWL
                     LSVMLIVIMPAMFLLPYAQQRGIGLETLAEFATTDKTAIFLQVLSLIPTHLVTLALAW
                     ALVTRLGKRPFFQTLGWTWSERFDLRKSVGLALGLYAVGFIIILIIGGPETQLAKIIS
                     SSRPTAFLTALMATATAPLVEEVIYRGLLYSGLRKTIGTAGAVVSVFLLFSLVHVPQY
                     WPHFGIMLTICLLSLVLTLVRAWTKSLLPCFIIHLVFNGVQSILIVFEPYFEKYLPER
                     LQGAHLSPLVEQFARLFA"
     gene            complement(6414..6581)
                     /locus_tag="AC159P_07170"
     CDS             complement(6414..6581)
                     /locus_tag="AC159P_07170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695271.1"
                     /translation="MDRGLRIEEAKKIDEAATIDEGIAVVNHRLTSVVAFSADPQSAI
                     HNPVALSPGVP"
     gene            complement(6591..7346)
                     /locus_tag="AC159P_07175"
     CDS             complement(6591..7346)
                     /locus_tag="AC159P_07175"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HIR3695272.1"
                     /translation="MPLIEHAPRTAEPRPPRALDRARSAIAIPLIYLYTVVMGTLSLA
                     SSFFDPEGRRQHWCASTWSRMIARTAGARVRVHGAEHLREGASYVFLSTHQSYMDIPA
                     MLGYLPAQLRIAAKREVFMIPFLGWHMRRAGHISINRGSTAEAVDALRRAASEVRAGV
                     SVFLFPEGTRSRDGALQAFKKGGFKFALAAGLPIVPVTIIGSRQVLPRDSITFRPGDV
                     EMYLDAPIPTAGLTDEDLPALMQSVRDAMAKHF"
     gene            complement(7400..7978)
                     /locus_tag="AC159P_07180"
     CDS             complement(7400..7978)
                     /locus_tag="AC159P_07180"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR3695273.1"
                     /translation="MDTSHKEQPEVLASEEVYAGHVVGLVVDTVREGDLTYKREVVRH
                     NGGAAVVAVFDDGTVALVSQYRHPAVRYVLELPAGRLEAGERPEESAARELEEELGVV
                     AGRMEQLSEFYTTPGFCAEKLWVFLATDLSETARRCEADEIIEVVRLPFARALEMIAS
                     REIEDAKTIIGLLLAAPRVGVATGDVAESTIP"
     gene            complement(7989..8330)
                     /locus_tag="AC159P_07185"
     CDS             complement(7989..8330)
                     /locus_tag="AC159P_07185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695274.1"
                     /translation="MYKRFLVTVAVFTAAHYAALLALRLLVLTLFVFGCAEEGPQPYV
                     VTDVVPLIERIAASMELPGVLVVVWLSSAGLPSAFGVALERLAVCANSPLWGVTLSAV
                     WFGLRRLPRFR"
     gene            complement(8338..8835)
                     /locus_tag="AC159P_07190"
     CDS             complement(8338..8835)
                     /locus_tag="AC159P_07190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695275.1"
                     /translation="MTDTPKKKGYGRVFVILLVVGLLVLVPLTWLALRMSRDAQRRRV
                     VNANEAAAIYTLEQIAAAEQLHFETYEGQYATFRQLLDAGLFKAPLEGDAAITSAGYT
                     FTLKLTPRTDAQGSFYSVNADPRESGTTGNRHFYIDSNITGMRFSEGRPATATDPPRL
                     TAETY"
     gene            complement(8846..>9236)
                     /locus_tag="AC159P_07195"
     CDS             complement(8846..>9236)
                     /locus_tag="AC159P_07195"
                     /inference="COORDINATES: protein motif:HMM:NF014874.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate ligase domain-containing protein"
                     /protein_id="HIR3695276.1"
                     /translation="APRVLYDGAHNEAGARALRAYLDEFVEAPVTLVFGAMRDKELGK
                     IGAALFPAARHLVFTRPDNARAAEVEELVRAVPVPAGSSTITLAPSSRDALTIARALA
                     GPEGIVCVTGSLYLVGEIKGWLNPLAR"
BASE COUNT         1484 a         3094 c         3284 g         1374 t
ORIGIN      
        1 gcttcacggc gctgtctctg aacggcgcgg tgacgctcgg cgagggcacg gacgattcct
       61 ccggcgcggc gccgcgcgag gtgcgcggga aattccagac gcgctggggc gcgggcgccc
      121 acgccgtcaa cctcttctcg ggccgcgggc gcatccggct cgccgcgctg tcgcgcgaag
      181 cgcaggaggc cgccgcgcgt tcgcacacgc cgaggccgcc gcgcatcgac gacccgagcg
      241 gcggcgaacg ggacgcgccc gtccgcaggc ccacgccgca gcccgccgag acgccgcagg
      301 aggtggacga agaggaggtg gtgcgcgtcg agtccgacct cgtgacgctc aacatcagcg
      361 tcgttgaccg cgcgagcggg cgcgggctgg cgggactcgc gcaaggagac ttcaggctgg
      421 ccgaagacaa cgtcgagcag gagatcgcgc acttcgaggc cgcggacgcg ccgttcgatc
      481 tgctgctcct catcgacctc tccggctcga ccgccaaagt cacggacatc atccgctcgg
      541 cggccctgcg cttcgtcgcc gctacccgcc cgcgcgaccg cgtcgccgtg ctgaccttcg
      601 ccgccgggac ggacatcgtc tcgccgctga cggacgaccg ccgcgcgctg cgcgaagcga
      661 tcagccgcat gaagcccccg accggtgaca cgcgcctcta cgacgcgctc gccggcgcca
      721 tggcctacct cgaacgcgag tccgcgccga cgcgccgccg cgccatcgtc atgatgagcg
      781 atgggctgga cagcacgatg ccgaacgtga cgggcgtcgg ctccgagctg ccttacgccg
      841 aggtgctcga ccgcgcgcgc gagttcgacg gcctgctcta cacgatctgg acgagcaccg
      901 agtacgaagc cttcagcccc gaagacatcc agcccgagac cttcgacctc gcccacgacc
      961 gcacgaaaga gttcgccgac gcgggcggcg gcgccttcta cctcgtcgag cggatggaag
     1021 atttggcggg cgcctacgag cgcgtcgtcg ccgacctcgg caccgtctac agcctctcct
     1081 accgccccac caacaagcag cgcgacggct cctggcgcgc catccgcgtc cgcctccccc
     1141 gccacgcggg cgccgtcgcc cgcggcaaac gcggctacta cgcgaagtga acaaaagccg
     1201 tgagccgtaa accgtaaacc gtgacaagtg aagaaccggt tttagccttt cactcttcac
     1261 acttcactcg tcactgcatt cttctcatca cgggttacgg ttcacggttc acggcctttg
     1321 aggttaagcg gcatcgccgg gacggtttcg ggcgtctaag acttactatg cgaaccgtgg
     1381 gaagagggtt gattttttat acggcaatcg tggcgttggt ggcgttggtg gtcgtccagt
     1441 tcgtcgcgtg cggcgataag ggcgcggagc cggcggcggc ggtggtgccg acggtggcgg
     1501 ctcggcggac tttgtcgccg ctgccggagc gtgcgctgag tccgcgtctg aaggcggcgc
     1561 gggagcgtgc gatgagggcg gcggcggcgg cgcgcggcct cgcctggaag ggcgaggtcg
     1621 ggatgacgga gctgtcgggt tgggagtacg gcacgcggac gaaggagatc gcggaggtgc
     1681 tcggcaccga ggacttgagc gggttcagca agctcgccgt ggcgggcggg atgctgcccg
     1741 aagggaccga cctcgcctcg ctcgccgtga cgttcacggc ggcgaccgcg ggcgccacct
     1801 actcgccgct cgacaagcag gtgatcctgc tcgcggagga cgcgaagcgc ggggcgccgg
     1861 ctaaccagtc gctcctcacg cacgagttcg tccacgcctt gcaggatcag cacttcgacc
     1921 tgctgaagac gctgctcgcg cgcccgttcg acttcgaccg gacggaggcg ctgttcgcgc
     1981 tcgtcgaggg cgacgcgatg aacgtcgagc ggcggatcga ggcgggcgag aaggagtggt
     2041 cgcggcgcac gctcgaagag atcgcgcggc acgaggacga gcgcttcgcg ccctatcgct
     2101 cgcagatcgg cgcgctcttc ccgccgcttt tgacggagac tttcatcttc cgctaccgcg
     2161 acggcgcgcg cttcgtcgag gcgatgcgac gcgcgcgccc cgcggtgggc gtggacgaca
     2221 tcttccgccg tccgcccgct tcgagcgagc aggtgctgca ccccgaaaag tatctcgcgg
     2281 gcgaagcgcc gcgcgaggtg gacgcgaacg cagagggcct gcgcgaagac ggctgggagg
     2341 ctctggcctc gacgccgctc ggcgagatcg gcgtgcgcgg gctgctgttg gcgggcgtcg
     2401 cggacgccga ggcgcgcaag gccgcagcgg gttggggcgg cgaccgcgct ttcgtcttca
     2461 gccgcgcggg gcgcgactcg gcgcaactct tcgtctggca gactgcgtgg gacgggcgcg
     2521 aggacgcacg cgagttcttc cgcgcttaca acacgctgct gcgtcgcggt cgcggcgccg
     2581 cgcaggcgga cggcggcgag gaggccgcgc tctggcgcga aggggagttg agcacgctcg
     2641 tgcggctcga aggcgacacg gtgctcgtcc tgcgcggcgc cgagcgggac gtggcggcgg
     2701 cgctgcggat agtgacgcgg cggtgagcgt tcgccgtgac gctcggcgcc gacggcgttt
     2761 ctcgttgatg ggataatcca gtcggaatcc ggattcgact cttgcgacgc gcgggcggga
     2821 gttgtatgct gtggctgtca attcttgctg actctccctg ccgacaacgg gaacgttccc
     2881 gaaaggaagg acgccatgag aaacactccg aggcataccg cgagctgcct cctcgcgctg
     2941 gttttcgcct ccgcctgcgc tgccgtgact gcgcagggac agacacgcga cctccgcatc
     3001 gtcaaggcgg tcgccggcgg ggtcaacttc gtctccggcg acgtgaagct tcaggcgccg
     3061 gggcggagcg gttggcaggc gatcacgatg aaggacgatt tgaagagcgg cgacatcgtc
     3121 aagaccggct cggacggtcg cgtcgaggtg ctgctcaacc ccggttccta cctgcggctc
     3181 ggcgtcaact cggagttcga gttgaccgac gcctcgctcg acaagctgcg ccttaaaatc
     3241 tacaagggga gcgcggtcgt cgaggcgacg ggctacgacg acatgctgct ctccatcgcc
     3301 atcgacacgc cgcgcaccga gctgagactc gtccgccgcg gcatctaccg catcaacgtc
     3361 acgcccaccg gcgagaccga gatcgccgtg cagaaggggc gggcgctcgt cggcaaagag
     3421 tcggcggcga cggtcgtcaa gggcggcaag gtggcccgcg tggacgcggg cggcgtcgag
     3481 gtggcgaagc tcgataagaa gaacctcgac gcgctcgacc tgtggagcaa ggagcgcgcc
     3541 gaggagctgg cgaagctgaa ccgcaggatc aaggccaagc agacgaacac gctgctcgcc
     3601 aacgccaact tcaaccgctt ctacgggcgg ttcggccacg gcgtctggct ctacgatccg
     3661 gtcagcaggt gctacacctt cctgcccttc ttcggctcgt ggagttcgcc ctacggctcg
     3721 ggctacggca gctactaccc ggcctacccc tgctactacg gcggctgcca ctcgtccggt
     3781 cgctggaaca acgacccgtg ggccggcaac atgccttcgt cgccgtcgcc caagtcgtac
     3841 gacccgccgc cgagcagctc accgccgagc ttctcgctgc cggtctcgcg cggctcgggc
     3901 ggcggagaca gcacggcccc ttccgccccc gccaggtcga agccgtaagg actgaacgcg
     3961 gcgacagccg ccgccgcgac cgaacaagaa ccgcatcgcc ccgccgcttg agcttcaacg
     4021 cggcggggcg atttcgttgg cgacgacgag ggccgcgtgc gtgacggcgg cgacgccgtt
     4081 gccggggaag accgtgtcgc cgaccatgaa gaggttggcg accgtcgtgc ggtgcgtcac
     4141 ggcgttcgcg ccgaaggcgt cgagcgcctg ccccgtgccg ccgaccatgc cgaacttgcg
     4201 ccgcgtctct tcgtagcagg tgcgcggcgt cgccgtctca aggacttcga cgccgccgcc
     4261 cagctccggc agcgccgcgt gcaggcgcgg ccaccaagcc tcaagcgccc gctgatcctg
     4321 ctcctcgtga tcgccctagt cctcgtggaa cgagaaccac ggctccgcct ccgtgaaggt
     4381 cgagacggtg acggcgcgtt tgcccgcggg cgcgcgggcg tcccaggcgg gcgcggcgct
     4441 gaacatgagg agcgcgcgct cggggtcgaa ctcgcgcccc tcctgccagt cgtcgagcgc
     4501 gaggatttgc tccgtcggga gacgcgccaa cgcctcttcg tccgcgctca ggaacatctg
     4561 gtaggcgccc cagccgcgca gcctcttcac gcgcgcgcgc gcctccgccg gactccggcc
     4621 gagcccgatc aacctgccgt aggtgtccca caccgtgagg ttgctgacga cggcgcgggt
     4681 cgcccccacg gtctcgccgc tcaacagatc gacgcccgtg gcccgaccgc tttcgtcgaa
     4741 agagaggcgc agcgccgtcg tgttgagacg caccgtgccg ccgctcttcg tgatcgactc
     4801 gacgagcgac gcggcgagcg cgcccgcacc gccgcgcatc gcgtacatcc cgcgcgtcgg
     4861 ctgcgaaagc gcgaccgccg cgtaagggta ggcgcagcgc tcgctcggaa cctgcgcgaa
     4921 gatttggagc tgcgcgtcca cgaagcggcg gaagcgcgcg gacgttccgg cgaggtgccg
     4981 cgccgccgtg tgttcgagtg aggctaagac gcgcggcgcg agaagcggct cgcgcgccgc
     5041 gagcatgacg cgctggagct tcgacgcggt ggccagcgca ggcagccgcg ccgcggcgcg
     5101 ttcgagcgcg cccgcgacct cagccgcctc gacgtagaag cgctccgcct cgcccgcgca
     5161 ttcggggaag gccgcgcgca cctccgccag atgttcgtcg cccgcgaggc cgacgcgcac
     5221 ctccgtcttg tccggcaggc ggacgacgta ggcggacggc gcggccctcc gcacttcggg
     5281 cggcgcgacc ggaagctccg cgaacacgcg ctcgtgaatc ccgcccggct gccaggaggc
     5341 gtacagcccc gcgccgcgct cgaaacggtg gcccgcgctc tcgaacggcg ccgcgcaccc
     5401 gccgacctgc gacccccttt cgagcaggca cacgtcaagc ccccgcgccg agagcagcgc
     5461 cgccgccgtc aggccgccga ggccgccgcc gacgatcacg acctcgtaag acatgagctt
     5521 aggcgaacag tcgagcgaac tgttcgacga gcggcgagag gtgcgcgccc tgcaaacgct
     5581 cggggaggta cttttcgaag tacggctcga agacgatgag gatcgactgc acgccgttga
     5641 agacgaggtg gatgatgaag caggggagca ggctcttcgt ccacgcgcgg acgagcgtga
     5701 ggacgaggct gagcaggcag atcgtgagca tgatgccgaa gtgcggccag tactgcggca
     5761 cgtgcacgag cgagaagagg aggaagacgg agacgacggc gcccgccgtg ccgattgttt
     5821 tgcgcaagcc cgagtagagc aggccgcggt agatgacctc ctcgacgagc ggcgccgtcg
     5881 ccgtcgccat cagcgccgtg aggaaggccg tcgggcgcga gctgctgatg atcttggcga
     5941 gctgcgtctc cgggccgccg atgatgagga tgatgatgaa gccgaccgcg tacagcccga
     6001 gcgcgaggcc gacgctcttg cgcaggtcga accgctcgct ccatgtccag ccgagcgttt
     6061 ggaagaaggg gcgcttgccg agccgcgtga cgagcgccca cgccagcgcg agcgtcacga
     6121 gatgcgtcgg gatgagcgag agcacctgaa ggaagatggc cgtcttgtcc gtcgtggcga
     6181 attcggccag agtctccagc ccgatccccc tctgctgcgc gtaagggagc aggaacatgg
     6241 ccggcatgat gacgatcagc atcacgctca ggagccacag gccgaccccg ccgggaatac
     6301 cccacggcgg gttatcgggg ttgatctcgt cggcgacggt cggcggcggc ggctcgaagc
     6361 tcgcgtacgt gctttgcgga atcggggtga taggcgtgct cacgcaatcg agtttaagga
     6421 actcccggcg aaagggcaac aggattgtgg attgcggatt gcggatcagc agaaaaggcg
     6481 accactgatg tcagacgatg gttgacgaca gcgatgcctt catcaatcgt tgccgcttca
     6541 tcaatcttct tcgcttcttc aatccgcaat ccgcgatcca caatccgcaa tcagaagtgt
     6601 ttcgccatcg cgtcgcggac ggattgcatg agggcgggga ggtcttcgtc ggtgaggccg
     6661 gcggtcggga tgggggcgtc gaggtacatc tccacgtcgc cggggcggaa ggtgatcgag
     6721 tcgcgcggca ggacttgtct ggagccgatg atggtgacgg ggacgatggg cagcccggcg
     6781 gcgagcgcga acttgaagcc gcccttcttg aaggcttgca gcgcgccgtc gcgcgagcgc
     6841 gtgccttcgg ggaagaggaa gaccgagacg cccgcccgca cctccgaggc ggcgcggcgc
     6901 aaggcatcga cggcctcggc ggtcgagccg cggttgatcg agatgtgccc ggcgcggcgc
     6961 atgtgccagc cgaggaaggg gatcatgaag acttcgcgct tggcggcgat gcggagctgc
     7021 gcgggcaggt agccgagcat tgccgggatg tccatgtagc tctggtgcgt cgaaagaaag
     7081 acgtacgacg cgccctcgcg caggtgctcg gcgccgtgga cgcgcacgcg cgcgcccgcg
     7141 gtgcgcgcga tcatgcggct ccacgtgctc gcgcaccaat gctggcgtct tccttcgggg
     7201 tcgaagaaag aggacgcgag cgagagcgtg cccatgacga ccgtatagag gtagatgagc
     7261 gggatggcga tggccgagcg cgcgcggtcg agcgcgcggg gcgggcgcgg ctcggcggtg
     7321 cgcggcgcgt gttcgatgag aggcaaagtg cccggactcc gctccgctcc gcgtgtgact
     7381 tgctgcgcgg tcttcgtcat cagggaatcg tcgattcggc cacgtcgccc gtcgccacgc
     7441 cgacgcgcgg cgcggcgagc agcaggccga tgatggtctt ggcgtcttcg atctcgcgcg
     7501 aggcgatcat ctcaagggcg cgcgcgaagg gcaggcgcac cacctcgatg atctcgtccg
     7561 cctcgcagcg gcgcgcggtc tccgacaggt cggtggcgag gaagacccac agcttctcgg
     7621 cgcagaagcc gggcgtggtg tagaactccg agagctgctc catccgaccg gcgacgacgc
     7681 ccagttcctc ctccagctcg cgcgcggcgc tctcctcggg ccgctcgccc gcttcgagcc
     7741 ggcccgccgg aagctccagc acgtagcgca ccgccgggtg ccggtactgg ctgacgagcg
     7801 ccaccgtgcc gtcgtcgaag acggcgacca ccgccgcgcc gccgttgtga cgcacgacct
     7861 cgcgcttgta agtcaggtcg ccctcgcgca ccgtgtccac cacgaggccg acgacgtggc
     7921 ccgcgtagac ctcctcgctc gccagcacct cgggctgctc tttgtgtgaa gtgtccatgt
     7981 gcgcccttct acctgaaccg ggggagacgc cgcagaccga accacacggc gctcagcgtg
     8041 acgccccaca aggggctgtt cgcacagact gcgagtcgtt cgagtgcgac gccgaacgcg
     8101 ctagggagac ctgcggaact gagccagacg acaacgagca cgcccggaag ctccatggac
     8161 gccgcgatcc gttctatgag tgggactacg tcggtgacga cgtaaggctg cggcccttcc
     8221 tccgcacaac cgaagacgaa cagcgttagt acgaggagcc gcagggcgag cagggcggcg
     8281 tagtgcgcgg cggtaaacac ggcgaccgtg actagaaatc tcttatacat acgcgtttca
     8341 atacgtttcc gcagtcaggc gcggcgggtc ggtggccgtc gcggggcgcc cctcgctgaa
     8401 gcgcatgccg gtgatgttgg agtcgatgta gaagtggcgg ttgccggtcg tgccgctctc
     8461 gcgcgggtcg gcgttgacgc tgtagaagga gccttgcgca tccgttctcg gcgtcagctt
     8521 gagcgtgaag gtgtagccgg cggaggtgat ggcggcgtcg ccttcgagcg gcgccttgaa
     8581 gagtcccgcg tccaagagtt gacggaaggt cgcgtactgc ccctcgtagg tctcgaagtg
     8641 gagctgctcg gcggcggcga tctgttcgag cgtgtagatg gcggcggcct cgttggcgtt
     8701 gacgacgcgg cggcgctgcg cgtcgcggct catcctcagc gcgagccagg tgagcgggac
     8761 cagcacgagc agccccacca ccagcaggat gacgaagacg cgcccgtagc ctttcttctt
     8821 cggtgtgtcg gtcatgcgtt tccggttagc gtgcgagtgg gttcagccag cccttgattt
     8881 cgccgacgag gtagagcgag ccggtgacgc agacgatgcc ctccggcccc gcgagcgcgc
     8941 gcgcgattgt cagcgcgtcg cggctcgacg gcgcgagcgt gattgtactc gaaccggcgg
     9001 ggacgggcac agcgcgcacg agttcttcga cctccgcggc gcgcgcgttg tcgggccgcg
     9061 tgaagaccag atggcgcgcg gcggggaaga gcgcggcgcc gatcttccca agctccttat
     9121 cgcgcatcgc gccgaagacg agcgtcacgg gcgcctcgac gaactcgtcg aggtacgcgc
     9181 gcagcgcgcg cgcgcccgcc tcgttgtgcg cgccgtcgta gagcacgcgc ggcgcg
//
LOCUS       DBNGOH010000364         6461 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_422216, whole genome shotgun sequence.
ACCESSION   DBNGOH010000364 DBNGOH010000000
VERSION     DBNGOH010000364.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6461)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6461)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6461
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_422216"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..518
                     /locus_tag="AC159P_07200"
     CDS             <1..518
                     /locus_tag="AC159P_07200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR3695277.1"
                     /translation="DDEEAARLYTRLHKRLVGFFRLRGLSDPGEAADATLDRAARKIA
                     GGAPVPDVGRYALGIARFVAKERLREEQREGAASAEFIDNLPGAFDEEAEAYYEQLKN
                     CFEQLGEEERKLLVAYCKVLRGRQRAEYRRRLAEQMRTTVVALRMRVTRLRSTLTDCV
                     RKQAGGELATI"
     gene            612..1424
                     /locus_tag="AC159P_07205"
     CDS             612..1424
                     /locus_tag="AC159P_07205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695278.1"
                     /translation="MTDERIVAYLLKELPDEELERFEDECFEQESWPAQLDAVEEDLI
                     EDYLREGLAPERRTRFEQNYLTTAARRERVRMAAALLRLLDERRTEAEDDEEAGEVVA
                     PPVAPVVPPPIAETWAERFRAFRGAPAWAPRAAAALALVLVAGVAWSLYNSRTPAPQT
                     VAALELNVRNSDRAAGTEQPGRVKLAPDTDALRVALTIPEGSPPAARYRVELENREGE
                     AKTTRVDGQEGGTVSVLIPAAQLARGRYVLKLFAVGADGTEQRLGSYFFNVE"
     gene            complement(1478..4645)
                     /locus_tag="AC159P_07210"
     CDS             complement(1478..4645)
                     /locus_tag="AC159P_07210"
                     /inference="COORDINATES: protein motif:HMM:NF024180.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing tetratricopeptide repeat
                     protein"
                     /protein_id="HIR3695279.1"
                     /translation="MSRMKAALIVPTLAAVLVLCPAPRLRGQEAPDAREAARQLAQAA
                     EDAAASVEGRLEALRKLEEAARLFLSVDETVEAARVLNRAGRLQLLLHEPYDALASHN
                     RALDFLRQRPAPEAEVDNLNGLGAAYMLLQQKEQAEGVLLKAIELSRLSGYTLGQAQA
                     LLTLSDKQNFYDHLLAVRTAQEALALWQSLGDKPGLARAYGKLGQYYMAQNLLPESMQ
                     NYERALGLWRELSDAREQAEALIMLGFIEHRRGEWSNSLALHAQARALIDERAEPYMM
                     GQIATTVAETFIKNGLPESGITHYERALEYFRLTKDFNAIATMVWGIGVAHYLSGNYP
                     AAAAHFQQALDAVEDNPLEAQCYEYLGRIHIANEAYDLALRNLQTAFAIYTRAGNLRE
                     AARARGLLGQIAERQGRLEQARREYRTALATFERLSDPFNQAAVYYALGKLELKRGAY
                     DAAEKYLRQSVEVTENVRRTPTSSDLTAAFSATVYERYEQYVECLMRRHEAQPGRGLD
                     ARAFETSELARARSLSELLRATQTALLPGLDRELAERERTLRHALRVKEDRRMALLVG
                     KADRKAELDALGREHAQLEAGYEQVAGAIRSRYPALEQMTRPTAWNLRRIQEQVVADD
                     QTVLLEYSLGEGRSYVWVVTRDGFKSRELAAQKQIDAAAQKVYRLLSAPPELAAAGEL
                     TSATRELARLVLAPVAAELNRRRVIVVADGALHYIPFQVLPSPSDVDEPLVAGHEIVN
                     APSASILGELREEAARRRPAAKTLAAFGDPVFESDYAQRKDAEGDGRQGAAPSLAMAR
                     WQQASRDIELDGDAFDPANIQSLFYAKRELANLRDMAGAEAFVAAEFDANRARLLGTD
                     LTEYAILHFATHGLLDPRRPENSGLVLSTVDREGRAQEGFVGLQDIYGLRAPVNLVVL
                     SACRTGLGKDVRGEGLLGLTRGFMYAGASGVVASLWKVNDEATSELMKQFYANMLRGG
                     MPPAAALREAQNSIRRRPAWRAPYYWAGFTLQGESRPVVRPARAEGAALSSSKVAGVV
                     VLSMLLAGVVWWYGRRRTRTAAARR"
     gene            complement(4677..6266)
                     /locus_tag="AC159P_07215"
     CDS             complement(4677..6266)
                     /locus_tag="AC159P_07215"
                     /inference="COORDINATES: protein motif:HMM:NF015380.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clostripain-related cysteine peptidase"
                     /protein_id="HIR3695280.1"
                     /translation="MMTDKIWKAETDFDGKPIKERIMETLKAKGISHEQPELEDHQPR
                     RAISTKCSVRAHAAVLAEIAERNRRFRQEPSPKDSLRSFLKFCSDNYPARHYMLFILG
                     HGVVVGNDVFLLDEHAAQSTLELKDLGVLLKEFRENIETRDGKKTNAKFELVGFHSCS
                     MSSLEVAFELQGTANYMLASQGPAFVGSWPYRQILIRIYNDLRTGFKNPATVDSEVKV
                     MLEKIFSYILFNSYDFVVAGYSFEACLSDLRRVEDVKGPVSRLAAALIAALGQKHPLA
                     RERILLAHLDAQSYFLENYTDLYDFCFCLRKRCTSDGSGKEEALLDPSLQEILEACGD
                     MIGVLEKGTVVDGDRFIVRSEFAGIMTQYSHGMSVYFPWTKPLHEKFLKDYQDYRFTA
                     DVKPTSWFAFLSKYLEATQRRPRAEEMASEVPVQSTKKGAAKARARSAKRTKNDRLGA
                     IQAELLEAFAARVFNVDGQLQGKPTPESSVGAGGDPVLIKNYPLFTRPPVNPTLVEEP
                     TKKDPATKRERSFLGELDEDS"
BASE COUNT         1020 a         2207 c         2115 g         1119 t
ORIGIN      
        1 cggacgacga agaagcggca cgcctctaca cgcgcctgca caagaggctg gtcggcttct
       61 tcaggctgcg gggcctctcc gaccctggcg aggccgccga cgcaacgctc gaccgcgcgg
      121 cccgcaaaat cgccggcggc gcgcccgtgc ccgacgtggg gaggtacgcg ctcggcatcg
      181 cgcgcttcgt cgccaaagag aggctccgcg aagagcagcg cgagggcgcg gcctccgccg
      241 agttcatcga caacctcccc ggcgccttcg acgaggaagc ggaagcttac tacgaacagt
      301 tgaagaactg tttcgagcag ttgggggagg aggagcggaa actgctggtg gcctactgca
      361 aggtcttgcg cgggcggcag cgcgccgaat accgccgccg tctggccgag cagatgagga
      421 cgaccgtggt ggccctgcgg atgcgcgtca cgcgcctgcg cagcaccctg acggattgcg
      481 tcaggaaaca ggcgggcggc gagctggcga cgatttagcc cgctacgaat ccacgatttc
      541 ggacatactc atataggagc accggagcga cggctccgtg gcggcccccg cgtaatgcaa
      601 gcaataaaag aatgactgac gaacggatag tcgcttatct gctgaaggag ctgcctgacg
      661 aagaactgga gcggttcgag gacgagtgtt tcgaacagga gagctggccg gcgcagctcg
      721 acgcggtcga agaagacctg atcgaagact acctgcgcga aggactcgcg ccggagcggc
      781 gcacgcgctt cgagcagaac tacctgacga ccgcggcccg ccgcgagcgc gtccgcatgg
      841 ccgccgcact cctgcgcctc ctcgacgaac gccggaccga agcggaagac gacgaagaag
      901 caggagaggt ggtcgcgccg ccggtggcgc ccgtcgtacc gccgccgatc gcggagacgt
      961 gggccgagcg gttccgcgct ttccggggcg cccccgcgtg ggcaccccgc gccgccgcgg
     1021 cactcgcgct ggtgctcgtc gcgggcgtcg cgtggtcgct ctacaactca cgcactccgg
     1081 cgccgcagac cgtcgccgcg ctggagctga acgtccgcaa cagcgaccgc gccgccggca
     1141 ccgagcagcc gggccgggtg aaactcgcgc ccgacaccga cgcgctgcgc gtcgccctga
     1201 cgatccccga agggtcgccg cccgcggccc gctaccgcgt ggagttggag aatcgtgagg
     1261 gggaggccaa gaccacgcgc gtggacggac aagaaggcgg aaccgtctcg gtgctgatcc
     1321 ccgcggcgca gctcgcgcgc gggcgctacg tcctgaagct cttcgccgtc ggcgcagacg
     1381 gcacggagca acgcctcggc agctatttct tcaacgtcga atagccgacc gcacggcggc
     1441 gagcgggccg cgcgcgattg cctccggcgc cgagcggcta gcggcgcgcc gccgcggttc
     1501 gcgttcggcg gcggccatac caccagacga cgcccgccaa cagcatcgac agcaccacca
     1561 ccccggcgac cttcgaggac gacaacgccg cgccctctgc gcgcgcgggc ctgaccaccg
     1621 ggcgggactc gccctgcaaa gtgaacccgg cccagtagta aggcgcgcgc cacgcgggcc
     1681 gccggcggat gctgttctgc gcctcgcgca acgccgccgc gggcggcatc cctccgcgca
     1741 gcatgttggc gtagaactgc ttcatcaact ccgaggtcgc ctcgtcgttc accttccaca
     1801 gactcgccac cacgcccgac gcgcccgcgt acatgaaccc gcgcgtgagg cctagcagcc
     1861 cctcgccgcg cacgtccttg cccagtcccg tccggcacgc gctcagcacg acgaggttga
     1921 ccggcgcgcg caaaccgtag atgtcttgca gcccgacgaa gccctcctgc gcgcgcccct
     1981 cgcggtcaac cgtcgagagc acgaggccgg aattctctgg ccgccgcggg tcgagcagcc
     2041 cgtgcgtggc gaagtggagg atggcgtact cggtcaggtc cgtgccgagc agccgcgcgc
     2101 ggttggcgtc gaactccgcg gccacgaaag cctccgcgcc ggccatgtcg cgcaggttgg
     2161 ccagctcgcg cttggcgtag aagagcgact ggatgttggc ggggtcgaag gcgtcgccgt
     2221 cgagttcgat gtcgcgcgaa gcctgctgcc aacgcgccat cgccagcgac ggcgcggcgc
     2281 cctgccggcc gtcgccctcc gcgtctttgc gttgcgcgta gtccgactcg aacacggggt
     2341 cgccgaaggc ggccagcgtc ttcgccgcgg gacgccgccg cgccgcctcc tcgcgcagtt
     2401 cgccgaggat ggaggccgac ggcgcgttga cgatctcgtg cccggcgacg agcggttcgt
     2461 caacgtccga cggcgacggc aagacctgaa acgggatgta gtggagcgcg ccgtcggcga
     2521 cgacgatgac gcgccgccgg ttcagctccg cagccacggg cgcgagcacc agccgcgcca
     2581 gctcccgcgt cgccgaggtc aattcgcccg cggcggccaa ctcggggggc gccgacagca
     2641 gccgataaac tttctgcgcc gccgcgtcga tctgcttctg cgcggccaac tcgcggctct
     2701 tgaagccgtc gcgcgtgacg acccacacgt agctccggcc ctcgcccaag ctgtactcca
     2761 aaagcactgt ctgatcgtcg gcgacgacct gctcctgtat gcgccgcaag ttccaggccg
     2821 tgggccgggt catctgctca agcgcagggt agcgcgaccg gatggctccg gcgacctgct
     2881 cgtaccccgc ttcgagctgc gcgtgttctc tacccagcgc gtccaactcc gccttccggt
     2941 cggccttccc gaccagcagc gccatccggc gatcttcctt gacccggagc gcgtggcgga
     3001 gcgtccgctc ccgctccgcc aactcgcggt cgaggccggg gaggagggcg gtctgcgtgg
     3061 cgcgcagcaa ctcggagagc gagcgcgccc gcgccagctc gctcgtctcg aaggcccgcg
     3121 cgtcgaggcc gcgccccggt tgcgcctcgt gccgacgcat cagacactcg acgtactgct
     3181 cataacgctc gtagacggtg gcggagaagg ccgccgtcag gtcgctgcta gtcggcgtgc
     3241 ggcggacgtt ctccgtgacc tcgacggact gccgcaggta cttttccgcc gcgtcgtaag
     3301 ccccacgttt cagttccagc ttccccagcg cgtaatagac ggcggcctgg ttgaaagggt
     3361 cggacagccg ctcgaaggtc gccagcgccg tgcggtattc ccgccgcgct tgttcgagcc
     3421 gtccctgccg ctcggcgatt tgccccaaca gcccgcgcgc ccgcgccgct tccctcaggt
     3481 tgccggcccg cgtgtagatg gcgaaggcgg tctgcaagtt ccgtagggcg aggtcgtacg
     3541 cctcgttcgc gatgtggatt ctgccgaggt attcgtagca ctgcgcttcg agcggattgt
     3601 cttccacggc gtcgagcgcc tgctggaagt gggccgcggc ggcgggatag tttcccgaaa
     3661 ggtagtgggc cacgccgatc ccccagacca tggtcgctat cgcgttgaag tctttcgtca
     3721 gccggaaata ctccagcgcc cgctcgtaat gcgtgatgcc gctttcgggc agcccgttct
     3781 tgatgaaagt ctcggccacg gtggtcgcga tctgccccat catgtacggc tcggccctct
     3841 cgtcgatcag ggcgcgggcc tgcgcgtgca gcgcgaggct gttcgaccac tcgcctcggc
     3901 ggtgctcgat gaagcccagc atgatcaaag cctcggcctg ctctctggcg tcgctcaact
     3961 cgcgccagag gccgagcgca cgctcgtagt tctgcatgga ctccggcaat agattctgcg
     4021 ccatgtagta ctggccgagc ttgccgtagg cgcgtgcgag gccgggcttg tcgcccagcg
     4081 attgccagag cgcgagagcc tcctgcgccg tccgcacggc cagcaggtgg tcgtagaaat
     4141 tctgcttgtc gctgagcgtg agcagcgcct gcgcctgccc gagggtgtag ccggacagcc
     4201 ggctcaactc gatggctttc agcaacaccc cctcggcctg ctccttttgc tggagcagca
     4261 tgtaggccgc gcccagcccg ttcaggttat cgacttccgc ctccggggcc ggcctctgtc
     4321 tgagaaagtc cagcgcccgg ttatgactgg cgagggcgtc gtacggctcg tgcaggagca
     4381 gttggaggcg gcccgcgcgg ttcaggacgc gggccgcctc caccgtctcg tccacgctga
     4441 ggaagaggcg cgcggcctct tccaactttc gcagcgcctc cagccgcccc tccacgctgg
     4501 ccgcggcgtc ctccgccgcc tgcgcgagct gtcgcgccgc ctccctcgcg tccggcgcct
     4561 cctgcccgcg caggcgcgga gccggacaca gcaccagcac cgccgccagc gtcggaacaa
     4621 tcaacgccgc tttcattcgg ctcatggcac aagcccccgc gctaaagtca gcggcctcaa
     4681 gagtcctcgt ccaactcgcc taagaaggac cgctctctct tagtggccgg gtccttcttg
     4741 gtcggttctt cgacgagagt cggattcacc ggcggtcgtg tgaacagcgg gtagttcttg
     4801 atcaacacgg ggtcgccgcc ggcgccgacc gaggattccg gcgtcggctt cccttggagt
     4861 tgcccgtcca cgttgaagac gcgggcggca aaggcttcga gtaattccgc ctgtatcgcc
     4921 ccgagcctat cgttcttcgt gcgtttcgcc gaccgcgcgc gagctttcgc cgcgcctttc
     4981 ttcgtgcttt gcaccgggac ttccgacgcc atctcctccg cccgcgggcg acgctgcgtc
     5041 gcttccaggt acttcgacag gaatgcgaac caggaggtcg gcttgacgtc cgccgtgaat
     5101 ctgtaatcct gatagtcttt caggaacttc tcgtgcagcg gcttggtcca tgggaagtag
     5161 accgacatgc cgtgcgagta ttgagtcatg atcccggcga actccgagcg gacgatgaac
     5221 cggtcgccgt ccaccacggt tcccttctca agcaccccga tcatgtcgcc gcaggcttca
     5281 aggatctcct gcaatgacgg gtctagcagc gcttcctcct tacccgatcc gtcggacgtg
     5341 cagcgcttcc ggaggcagaa gcagaaatcg tagaggtctg tgtagttctc cagaaagtaa
     5401 ctctgcgcgt ccaggtgagc cagcaggatg cgctcgcgcg ccagcgggtg tttctggccg
     5461 agggcggcga tgagggccgc ggccagccgg gagaccggcc ccttcacgtc ctccaccctc
     5521 ctcaagtcgc tcaggcatgc ttcaaaggag tagcccgcca cgacgaagtc gtagctgttg
     5581 aataggatgt aggagaaaat cttttccagc atcaccttga cttcgctatc gaccgtcgcc
     5641 gggtttttga atcctgtccg caggtcgttg tagatgcgga tcagtatctg gcggtagggc
     5701 caactgccga cgaacgccgg gccttgcgag gcgagcatgt agttggccgt gccctgaagc
     5761 tcgaaagcca cctccagcga actcatcgag cagctgtgga agccgaccag ctcgaacttg
     5821 gcgttggtct ttttcccgtc cctcgtttca atgttctccc tgaactcctt cagcaggacg
     5881 ccgaggtctt tcaactccag cgtggactgc gcggcgtgct cgtcgagcag gaacacgtcg
     5941 ttgccgacca ccacgccgtg cccgaggatg aagagcatat agtgccgggc cgggtagttg
     6001 tcgctacaga atttcaggaa gctccgtaaa gaatctttag gggacggttc ttgccggaac
     6061 cgtcggttcc gttcggcgat ctcggcgagc acggcggcgt gtgcccgcac ggagcatttg
     6121 gtggagatgg cgcggcgagg ctgatggtcc tccagttccg gctgttcgtg gctgattccc
     6181 ttggccttaa gggtttccat gatccgctct ttgatgggct tgccgtcgaa gtccgtttcg
     6241 gccttccata tcttgtcggt catcatgttg acgacgaagg ggtcgttggg gctgaacccg
     6301 aggaagccga tcttgtggtc tttggcgcgg cgtttggccc ctcgcccttt gctccttcgc
     6361 cctttggtct tgtgtccgtt gccggcttcg agtttgttga tgcggttgat gtcgaagatg
     6421 tgagtcgagg tgttctcggt gtgcgggtcg aactgcgcga c
//
LOCUS       DBNGOH010000365         3463 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4454342, whole genome shotgun sequence.
ACCESSION   DBNGOH010000365 DBNGOH010000000
VERSION     DBNGOH010000365.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3463)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3463)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3463
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4454342"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..421
                     /locus_tag="AC159P_07220"
     CDS             <1..421
                     /locus_tag="AC159P_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011143103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695281.1"
                     /translation="EAEEAVEHRSKKEQILELYEAGTGDIAEIVRRVKARPSYVAQVL
                     QSAGHLEGYFDLYTTTGKEQNVYTRFFRNVLSFKTVEAARESVQRIDRLYNYFERLGD
                     RAGQHQAMVLALTGKNRARWSGKQEEALVFSEWLAAH"
     gene            825..1226
                     /locus_tag="AC159P_07225"
     CDS             825..1226
                     /locus_tag="AC159P_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005990379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR3695282.1"
                     /translation="MSDNAMPSPAHGTFCWNELGSTDDEAAKKFYSELLGWTYKEGDA
                     GGMKYSEITAGGRPVGGVYKMGPEFGEAPSHWMAYVAVDDVDASAKRVEELGGKVCVP
                     PTDIPNVGRFSVINDPTGATLSLITLKGVHS"
     gene            1324..1821
                     /locus_tag="AC159P_07230"
     CDS             1324..1821
                     /locus_tag="AC159P_07230"
                     /inference="COORDINATES: protein motif:HMM:NF019932.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR3695283.1"
                     /translation="MRIRLSLVLFVLFIFAAAWSVAVISGYASSNAAANGEIARAIIA
                     REKASAEAWKRKDKAFFADFLADDATYFGWMSPYLETDPKENFLPKFEQVAEMMKIID
                     FQMYNPRVQVYGDTAVLTYNAASTISFGGQTMNYTCKMTSVYVKQGATWRVVHGHESM
                     NPGAR"
     gene            1862..2299
                     /locus_tag="AC159P_07235"
     CDS             1862..2299
                     /locus_tag="AC159P_07235"
                     /inference="COORDINATES: protein motif:HMM:NF020157.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1761 domain-containing protein"
                     /protein_id="HIR3695284.1"
                     /translation="MNINYAAVVASALAYWVLGAVWYGVFSKPFIALMRWTPEQLARI
                     EAEGAGREIGVALLTSLLTAYVLAHFVRFTGADTVSKGAQTGFFVWLGFVLTTNLGTV
                     LFEFRPLGLYLINNGYHLVGLLGMGALLAVWKRKEARVAAYQT"
     gene            2697..3362
                     /locus_tag="AC159P_07240"
     CDS             2697..3362
                     /locus_tag="AC159P_07240"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012926503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HIR3695285.1"
                     /translation="MPARNEDLHGNVPDKADVALLLIDVINDLEFESGEELLRHALPM
                     AERLAELKKRARAARIPAIYVNDNFGKWQSDFNRILKHCLEDDVVGRPVAELLRPDEE
                     EDYFVLKPKHSGFFSTQLDILLDYLEVKTLILTGMAGDICVLFTANDAYMRDFNLVIP
                     EDCVASTDPGENRHALEMMRRLLKAETRPSTELDLEELKREAARVDEPNPAPQSQQFA
                     EKN"
BASE COUNT          650 a         1116 c         1107 g          590 t
ORIGIN      
        1 ggaggcggag gaagcggtcg agcaccgcag caagaaggaa cagattctag agctgtacga
       61 ggccgggacg ggcgacatcg cagagatcgt gcgccgcgtg aaggcgcggc cctcttacgt
      121 cgcgcaggtc ttgcagtcgg caggccacct cgaaggttac ttcgatcttt acacgacgac
      181 gggcaaggag cagaacgtct acacgcgctt cttccgcaac gtcctctcgt tcaagacggt
      241 cgaggccgca cgcgagagcg tgcagcgcat cgaccggctc tacaactatt tcgagcgcct
      301 cggcgaccgc gcgggccagc atcaggcgat ggtcctggcc ctgacgggta agaaccgcgc
      361 ccgttggagc ggcaagcagg aggaggcgct ggttttcagt gaatggctcg ccgcgcatta
      421 agggccaggc aggttttaag aaggaacaca aagcaagtcc cacgcaagtc ggcagcagta
      481 agaacgagca aggggtgggt ttgaaaggtg agtttttcag gcgtgggaaa ctcacctttc
      541 aacgtttccg ccggctgacg aaagccgccg ctcgttcgag ttcgtcaagt tcccttccct
      601 cgcagtcgga cgacctcgca cgcggcctgt agttcgacac aggcttttct tttcatcctc
      661 gcttttgttg tccggcggcc cccggcccgg actgtggaga agtgtaccgt gggcgcgaac
      721 gtagttcggt cgcgcccgca gtccgcactt ttacggcggg cgccggtcac ttcggttttc
      781 gaattctttg cgacacgaat cagaccttaa aggagacaag agacatgagt gataacgcga
      841 tgcctagccc cgcgcacggc accttctgct ggaacgagct gggttcgacc gacgacgagg
      901 cggcgaagaa gttttactcg gagctgctcg ggtggactta caaggagggc gatgcgggcg
      961 gcatgaaata ctcggagatc acggcgggcg ggcggcccgt cggcggcgtc tacaagatgg
     1021 ggccggagtt cggcgaggcg ccgtcgcact ggatggccta cgtcgccgtg gacgacgtgg
     1081 acgcttcggc gaagcgcgtc gaagagctgg gcgggaaggt ctgcgtgccg ccgaccgaca
     1141 tcccgaacgt cggtcgcttc tcggtcatca acgacccgac cggcgccacg ctctcgctca
     1201 tcacgctcaa gggcgtgcac tcgtaaggcg cggcgcgccg ggcgatgaaa aaacgcctcg
     1261 ccgctcgatg cgacacggag aagcccccct tccccctaac agactttgga aggagtaagc
     1321 atcatgagaa tccgactctc cctcgtcctg ttcgtcctgt tcatcttcgc cgccgcgtgg
     1381 agcgtcgccg tcatcagcgg ctacgcctcg tcgaacgcgg cggccaacgg cgagatcgcc
     1441 agggccatta tcgcccgcga gaaagcctcc gcagaggcgt ggaagcgcaa ggacaaggca
     1501 ttcttcgccg actttctggc cgacgacgcg acctatttcg gctggatgag tccttacctc
     1561 gaaaccgacc cgaaggagaa tttcctcccc aagttcgagc aggtcgccga gatgatgaag
     1621 atcatcgact tccagatgta caacccgcgc gtgcaggtct acggcgacac cgcggtgctc
     1681 acctacaacg ccgcttcgac gatcagcttc ggcggccaga cgatgaacta cacgtgcaag
     1741 atgacgagcg tctacgtcaa acagggcgcg acgtggcgcg tcgtccacgg ccacgagtcg
     1801 atgaaccccg gcgctcggtg agcgcccccc gccacagtcc ccgaaaggag tgaactcttt
     1861 catgaacatc aactacgccg ccgtcgtcgc gtcggcgctc gcctactggg tgctcggcgc
     1921 cgtgtggtac ggcgtcttct cgaagccgtt catcgcgctc atgcgctgga cgccggagca
     1981 actggcgcgc atcgaggcag agggcgcggg ccgcgagatc ggcgtcgcgc tgctcacctc
     2041 gctgctgacg gcctacgtgc tcgcgcactt cgtccggttc acgggcgccg acacggtttc
     2101 gaagggcgcg cagacgggct tcttcgtctg gctcggtttc gtgctgacga ccaacctcgg
     2161 caccgtgctc ttcgagttca ggccgctcgg cctctacctc atcaacaacg gctaccacct
     2221 cgtcggcctg ctcggcatgg gggcgctgct ggccgtctgg aagaggaagg aggcgcgcgt
     2281 ggccgcctac cagacttgag gcggcgaaag atgaagcgtt tcgcggacga cgggacggcg
     2341 cttcggccag ggggcgcgca cacaacagcg cgcgagcatg cgacggcagg ttcaggcgac
     2401 tcactcctac ctcaagcctg tgcttcggcg cgtttcggcc tctgcgcgcc gccgtgctcg
     2461 cgcgtgccgg gcgggcgcgc ttgccttcgg gctgcggcgt ccttgcctct cgtgcgtgag
     2521 acgctagagg ggggcgaggg gcgagcggga aaacggtcaa gggtgggtta cgccatctta
     2581 ctttttcccc gcggcccctt gccccttgcc ccttgccttt tgcctttccc cgccgccgca
     2641 cgcataatcc acatgacccc cgaaaaattt cgtcagaccg tagagggact tccgacgtgc
     2701 ctgcgagaaa cgaagacctg cacggcaacg tgccggacaa ggcggacgtg gcgctgctgc
     2761 tgatcgacgt gatcaacgac ctcgaattcg agagcggcga ggagctgctc aggcacgcgc
     2821 tgccgatggc cgagcggctg gccgagttga agaagcgggc gcgcgcggcg cgcatccccg
     2881 cgatctatgt gaacgacaac ttcggcaagt ggcagtcgga cttcaacagg attttgaagc
     2941 actgcctgga agacgacgtg gtgggccgac ccgtcgcgga gctgctgcgg cccgacgagg
     3001 aggaggacta cttcgtgctg aagccgaagc actcgggctt cttctccacg cagctcgaca
     3061 tcctgctcga ctacctcgaa gtcaagacgc tcatcctcac cggcatggcg ggcgacatct
     3121 gcgtcctctt cacggcgaac gacgcctaca tgcgcgactt caaccttgtc atcccggaag
     3181 actgcgtcgc ctcgaccgac cccggcgaga accgccacgc gctcgaaatg atgcgccggc
     3241 tgctcaaggc cgagacgcgg ccctcgaccg aactcgacct cgaagagctg aagcgcgagg
     3301 ccgcgcgcgt ggacgagccg aaccctgcgc cgcagtcgca gcagttcgcg gagaagaact
     3361 gaccacagcc ctcacaccaa gccgtcgcac caagtcggtc gcgcgaaagt gaccgcatcg
     3421 ttcttgaagg cgccgccgcc gccgcatcgc ttcgtcctga cga
//
LOCUS       DBNGOH010000366         5127 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_605321, whole genome shotgun sequence.
ACCESSION   DBNGOH010000366 DBNGOH010000000
VERSION     DBNGOH010000366.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5127)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5127)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5127
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_605321"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..320
                     /locus_tag="AC159P_07245"
     CDS             <1..320
                     /locus_tag="AC159P_07245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="endonuclease III domain-containing protein"
                     /protein_id="HIR3695286.1"
                     /translation="AVEYLSRFRGIGPKTVACTLLFACRKDVFPLDTHIFRILRRVGL
                     LPHKLTDARAHALLTALVPRGKFYSFHVNLIRHGRRLCRPSDPLCERCPIVEYCDYGQ
                     EMI"
     gene            438..1373
                     /locus_tag="AC159P_07250"
     CDS             438..1373
                     /locus_tag="AC159P_07250"
                     /inference="COORDINATES: protein motif:HMM:NF020279.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HIR3695287.1"
                     /translation="MKCNYCGHELTPFTKFCTKCGNPAPQPSQAGYTPPPAAPQPPPA
                     SWGAGAQSAPRQQPRRKSRAGKILLIVFTVLAVLGVGAGALAYFGFKALKGSIKSSEA
                     YSVAERELRESRRAADVLGEIKETGFPIGTYKQEADGTGQAAFTLSVVGTKASGRYFV
                     TMHREGGTWHVTHGLLQIDGREESIDIMEEKGAGPTGPTGPEINAPPPPPPPPGGTGG
                     KTISGGVLNGKAISKPQPPYPAIAKAAKAQGTVTVQVVVDEEGKVISASAVGGHPLLQ
                     AAAVQAARQARFSPTKLSGKPVKVSGVVTYNFVLE"
     gene            1386..1868
                     /locus_tag="AC159P_07255"
     CDS             1386..1868
                     /locus_tag="AC159P_07255"
                     /inference="COORDINATES: protein motif:HMM:NF017774.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HIR3695288.1"
                     /translation="MSYLVDGNNVMGQRVGWHRDKPGARRRLLTELARFSRAAGVTVE
                     AVFDGAPEEFFPDGSHFMGVRVYYAARGSDADTRIKRLVEDSRERRTLVVVTSDRGLA
                     DYVRRCGARVVAAGAFRRRLDETCADDEKAAGRAPQDGARREDLEEWMHYFGVAPEDE
                     "
     gene            complement(2045..2482)
                     /locus_tag="AC159P_07260"
     CDS             complement(2045..2482)
                     /locus_tag="AC159P_07260"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009195898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HIR3695289.1"
                     /translation="MTEQAKKGDAVRVHYTGRLEGGEVFDTSEGGEPFEFVVGTGQAI
                     PGFDAAVDGLGLGERRRVEIEPEDAYGPHRDGLVARLPRETAQFPTEPQVGANFIMPL
                     PDGQQLEVVVTEVTDTHITIDGNHPLAGRKLIFDVELVEIKEQ"
     gene            2726..4981
                     /locus_tag="AC159P_07265"
     CDS             2726..4981
                     /locus_tag="AC159P_07265"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HIR3695290.1"
                     /translation="MAQTSRQEPAQPPSAAAQQQQPQPRPSPSAQDDDVVRITSNLVQ
                     FDAVVTDKNGRHVIDLGAEDFEILIDGKRQEITNFSYVSNEAGAGVPTPTPKKNPDKN
                     APPAPPVRLRPEKVRRTIALVVDDLGTSFESTYYVREALKKFVDQQMQPDDLVAIIRT
                     SAGVGALQQFTSDKRMLHAAIERVRWNPSGRAGIGAFAPMAADPLARVRADGDEGTAR
                     TAGGRDTENLGAGDVDEFREEIFSVGTLGALNYVVRGLNDLPGRKSVIMFSDGFRIHR
                     RDDSSFNQRVVESLRRLTDLANRASVVIYTIDARGLQTLGLTAADNTAGMTGAEIEQN
                     LMERRNALFDTQDGLNYLAQQTGGFFIKNSNDLAGGVRKVIEDQRGYYLIGFRPGDEV
                     FDATRIRFNKIKVNLLRPGLKTRTRGGFYGFTEETARPVRRTREEQIMAALTSPFGSG
                     EIPLRVTSIYSNEGEKNADFLNSLCHIDASKLKFEPEADGWHKAVFDVAAITFGADGR
                     VVDEISRIETVRARHEALESVLRGGLIFSMRVPVKKPGAYQLRVAVRDEASERVGSAS
                     QFVEVPNLKKKRLALSGIIMRGTPTAAAAHNRSAPATGNDDAGAGNAAAAAGNSNAAR
                     QGVVPEGPQLEADPATHAAVRRFKQGSQADFFYYIYNAKLDRAAQRPQLTTQFRLFRD
                     GQPVHTGTAVPYNPDTQSDMDRLIMGTRIRFNAALAPGEYVLQVIVTDALAKDPKHRT
                     ATQWVDFEVVK"
BASE COUNT          908 a         1804 c         1665 g          750 t
ORIGIN      
        1 aagcggtcga gtatctttcg cgcttccgcg gcatcggccc caagacggtc gcctgcacgc
       61 tcctcttcgc ctgccgcaag gatgtcttcc ccctcgatac gcacatcttc cgcatcctcc
      121 gccgcgtcgg cctcctcccg cacaagctta cggacgcgcg cgcccacgcg ctcctcaccg
      181 ccctcgtccc ccgcggcaag ttctactcct tccacgtcaa cctcatccgc cacgggcgcc
      241 ggctctgccg ccccagcgac ccactgtgcg agcgctgccc catcgtcgag tactgcgact
      301 acgggcagga gatgatttga aacacgcgcc gtaattagtg gcgtatagat tcccgtctgg
      361 tcttataatc actcaccgat agacggtcgc ctcgcccacg cacggcgaac gagttcgaat
      421 ccccggagga cggaacgatg aagtgcaatt actgcggcca cgagctgacg ccgtttacga
      481 agttctgcac gaagtgcggg aaccccgcgc cgcaaccgtc gcaggccggc tacacgccgc
      541 cgcccgccgc gccacagccg ccgcccgctt cgtggggcgc gggcgcgcag agtgcgcccc
      601 gccagcagcc gcggcgcaag tcgcgagcgg ggaaaatcct gctcatcgtc ttcactgtcc
      661 tggcggtgct cggcgtaggc gcgggcgcgc tcgcctactt cggcttcaag gcgctgaagg
      721 ggagcatcaa gtcttcggag gcgtacagcg tggcggagcg cgagctgcgg gagagccggc
      781 gggccgccga cgtgctcggc gagatcaaag agacgggttt ccccatcggc acttacaagc
      841 aggaggcgga cggcacgggc caggcggcct tcaccttgtc ggtcgtgggg acgaaggcga
      901 gcgggcgcta cttcgtcacg atgcatcgcg agggcggcac gtggcacgtc acgcacggac
      961 tcttacaaat cgacggcaga gaagagtcga tcgacatcat ggaagaaaag ggcgcggggc
     1021 cgacggggcc gacggggccg gaaatcaatg ccccgccgcc gcctcctcct cctccgggcg
     1081 gcacgggcgg caagacgatc agcggcggcg tcctgaacgg caaggcgatc agcaagccgc
     1141 agccgccgta cccggcgatt gcgaaggcgg cgaaggcgca gggaactgtg acggtgcagg
     1201 tcgtcgtgga cgaagagggg aaggtcatct cggcctcggc ggtgggcggc cacccgctct
     1261 tgcaggcggc ggcggtgcag gccgcgcggc aggcccgctt ctcgccgacg aagctctccg
     1321 gcaagccggt caaggtctcg ggcgtcgtca cctacaactt cgtgctggag taggagcgcg
     1381 cggcggtgtc gtacctcgtg gacggcaaca acgtgatggg gcagcgcgtc ggctggcaca
     1441 gggacaagcc gggcgcgcgc cgtcgcctgc tcaccgagct ggcgcgcttc tcgcgcgcgg
     1501 cgggcgtgac ggtggaggcc gtcttcgacg gcgcgcccga agagttcttc cccgacggct
     1561 cgcacttcat gggcgtgcgc gtctactacg ccgcgcgcgg ctccgacgcc gacacgcgca
     1621 tcaagcggct cgtcgaagac tcgcgcgagc ggcgcacgct cgtcgtcgtc acctccgacc
     1681 gagggctggc ggactacgtg cgccgctgcg gcgcgcgcgt cgtcgccgcg ggcgcgttcc
     1741 gccgccgtct ggacgagacc tgcgccgacg acgagaaggc cgccggccgt gctccccaag
     1801 acggcgcgcg gcgcgaagac ctcgaagagt ggatgcacta cttcggcgtc gcgcccgaag
     1861 acgagtaggg aggaaacagt aggcagtagg cagcaggcag taggcagcca ggacataaga
     1921 cagaagagtg aagaagcttg agaggtaaag tgcaacgcct ctccactctt catgcgatga
     1981 agcaagaaga ggcttgagac cttacatctc aagcctctcg cttttactgc ctaccgccta
     2041 ctgcctactg ttcttttatc tcgaccagct ccacgtcgaa gatgagtttg cgaccggcga
     2101 gggggtggtt gccgtcgatg gtgatgtggg tgtcggtgac ttcggtgacg acgacttcga
     2161 gctgctggcc gtcgggcagc ggcatgatga agttggcgcc gacctgcggc tcggtgggga
     2221 actgcgcggt ctcgcgcggg aggcgcgcga ccagcccgtc gcggtgcggc ccgtaggcgt
     2281 cttcgggttc gatctcgacg cggcgccgct cgccgaggcc gaggccgtcc acggcggcgt
     2341 cgaagccggg gatggcctgc cctgtgccga cgacgaactc gaacggctcg ccgccctccg
     2401 aggtgtcgaa gacctcgccg ccttccagcc gtcccgtata atggacgcgc acggcgtcgc
     2461 cctttttggc ctgttctgtc ataatcggat tctcctcggc taagttcggc gcgagaatcg
     2521 cacacccgac tcgcaaacgc cagccgagag aatgaaaccg cgagcacgac gccgtcgtgg
     2581 aaggcacgcg acgcacgcga cgcctcccgt tgagcttgaa cgaccccgaa caacacgacc
     2641 ccgatttgga tttaccatga aacgccatcc ctcattcgcc ttgcccctct ccctgcttct
     2701 ctccctgtcc gcgctcgcgc ccgccgtggc ccagacaagt cggcaggagc cggcgcagcc
     2761 gccgtccgcc gccgcgcaac aacagcaacc gcagccccgg ccctcgccgt cggcgcagga
     2821 cgacgacgtg gtgcggatca cttcgaacct cgtgcagttc gacgccgtcg tgacggacaa
     2881 gaacgggcgg cacgtgatag acctcggcgc cgaagacttc gaaatcctga tcgacggcaa
     2941 gcggcaggag atcaccaact tctcctacgt ctcgaacgag gcgggcgcgg gcgtgcccac
     3001 gccgacgccg aagaagaacc cggacaagaa cgccccgccc gcgccgcccg tgcgcctgcg
     3061 gcccgagaag gtgcggcgca ccatcgcgct cgtggtggac gacctcggca cctccttcga
     3121 gagcacgtac tacgttcgcg aggcgctcaa gaagttcgtc gatcagcaga tgcagccgga
     3181 cgatctggtg gccatcatcc gcacgagcgc gggcgtcggc gcgctccagc agttcacctc
     3241 cgacaagcgc atgctccacg ccgccatcga gcgcgtgcgc tggaaccctt cgggccgcgc
     3301 gggcatcggc gccttcgcgc ccatggccgc cgacccgctg gcgcgcgtgc gcgccgacgg
     3361 cgacgagggg acggcccgca ccgccggcgg gcgcgacacg gagaacctcg gcgcgggcga
     3421 cgtggacgag ttccgcgagg agattttttc ggtcggcacg ctcggcgcgc tcaactacgt
     3481 ggtgcgcggg ctgaacgacc tgccgggccg caagtcggtc atcatgttct cggacggctt
     3541 ccgcatccac cggcgcgacg acagctcgtt caaccagcgc gtcgtcgaaa gcttgcgccg
     3601 cctgaccgat ctggccaacc gtgcctcggt cgtcatctac accatcgacg cgcgcgggct
     3661 gcaaacgctc ggcctgacgg cggcggacaa caccgccggg atgacgggcg ccgagatcga
     3721 gcagaacctc atggagcgcc gcaacgcgct gttcgacacg caggacggct tgaactacct
     3781 cgcgcagcag acgggcggct tcttcatcaa gaactcgaac gacctcgcgg gcggcgtccg
     3841 caaagtcatc gaagaccaga ggggctacta cctgatcggc ttccgccccg gcgacgaggt
     3901 cttcgacgcc acgcgcatcc gcttcaacaa gatcaaggtg aacctcctgc gccccggcct
     3961 gaagacgcgc acgcgcggcg gcttctacgg cttcacagag gagacggcgc ggcccgtccg
     4021 ccgcacgcgc gaggagcaga tcatggccgc gctcacctcg cccttcggct cgggcgagat
     4081 acccctgcgt gtcacctcga tctacagcaa cgagggcgag aagaacgccg acttcctcaa
     4141 ctcgctctgc cacatcgacg cctcgaagct caagttcgag ccggaggccg acgggtggca
     4201 caaggccgtc ttcgacgtgg ccgccatcac cttcggcgcc gacggcaggg tcgtggacga
     4261 gatcagtcgc atcgagacgg tgcgcgcacg gcacgaggcc ctcgaatcgg tgctgcgcgg
     4321 cggcctcatc ttctcgatga gggtgccggt caagaagccg ggcgcgtacc aactgcgcgt
     4381 cgccgtccgc gacgaggcca gcgagcgcgt cggctcggcc agccagttcg tcgaggtgcc
     4441 gaacctcaag aagaaacggc tcgcgctctc cggcatcatc atgcgcggca cgccgacagc
     4501 cgccgccgcc cacaaccgca gcgccccggc caccggcaac gacgacgccg gtgccggcaa
     4561 cgcggcagcc gccgccggca acagtaacgc ggcgcggcag ggcgtggtcc ccgaaggccc
     4621 gcagctcgaa gcggacccgg cgacgcacgc cgccgtgcgc cgcttcaagc agggttcgca
     4681 ggccgacttc ttctactaca tctacaacgc gaagctcgac cgcgccgcgc agcgcccgca
     4741 gctcacgacg cagttccgcc tcttccgcga cggccagccc gtccatacgg gcaccgccgt
     4801 cccctacaac cccgacaccc agtccgacat ggatcgcctc atcatgggca cccgcatccg
     4861 cttcaacgcg gcgctcgcgc ccggcgagta cgtccttcag gtcatcgtca ccgacgcgct
     4921 cgccaaagac cccaagcacc gcaccgcgac gcagtgggtc gatttcgaag tcgtcaaatg
     4981 acgtactccg tgagccggta aaagccgtga accgtgaacc ggaaaccgtg acaagtaaaa
     5041 gactgtaagc cgtgaaccgt gacgagtccg aaccggtttt cacctgtcac ggttcacggt
     5101 tcacggctta cggcttttgg aggtgtt
//
LOCUS       DBNGOH010000367         6919 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_787931, whole genome shotgun sequence.
ACCESSION   DBNGOH010000367 DBNGOH010000000
VERSION     DBNGOH010000367.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6919)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6919)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6919
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_787931"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..349
                     /locus_tag="AC159P_07270"
     CDS             <1..349
                     /locus_tag="AC159P_07270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695291.1"
                     /translation="RTGPARMELLKRYWFEFDMSGLSDEQLRRYPYFPAYGVGVTAFD
                     YEDALRLIRKWVLQDRYDMPEIAKVTEGVDVSQLLERPFARTRLLPHLGCPVWRGVWH
                     PAFNLWYGADTEL"
     gene            491..931
                     /locus_tag="AC159P_07275"
     CDS             491..931
                     /locus_tag="AC159P_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011552056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HIR3695292.1"
                     /translation="MDWQENIIEAGGGIKHLLAETRRIAVLGIKTEQQSYQPAFYVPQ
                     YMANAGFEVIPVPVYYPEVTRILGREVYRKLVDVPGEVDLVNVFRRPQDIEAHVEDIL
                     AKRPRAVWFQSGIRHDAAAERFARAGIKVVQDRCLMVEHRMAAR"
     gene            1114..3255
                     /gene="glgX"
                     /locus_tag="AC159P_07280"
     CDS             1114..3255
                     /gene="glgX"
                     /locus_tag="AC159P_07280"
                     /EC_number="3.2.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120549 - limit dextrin
                     alpha-1,6-maltotetraose-hydrolase activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HIR3695293.1"
                     /translation="MKVWPGQPYPLGATWDGAGVNFALFSENATAVELCLFDSADAVQ
                     ESRRVRLTEHTDQVWHAYLPEARPGLLYGYRVRGPHEPRAGHRFNPRKLLIDPYAKAI
                     SGTVRWDEAVFGYTIGHELRDLSLDTRDSAAHVPRCVVVDNSFDWGDDTRPRTPWHRT
                     LIYELHVKGFTMRHPEVPEELRGTYLGLAHPSVIDYLKSLGVTAVELMPVHQFISDKF
                     LVDRGLTNYWGYNSIGFFAPDVRYSAGGALGAQVSEFKTMVRTLHREGIEVILDVVYN
                     HTGEGSHMGPTLSFRGIDNAAYYRLAHADRSRYVDYTGCGNTLNMTHPRTLQIIMDSL
                     RYWVQEMRVDGFRFDLAAALARELHEVDRLGAFFDIIHQDPVLSQVKLIAEPWDLGEG
                     GYQVGNFPVLWAEWNGVYRDAVRGYWRGDSGLIDDLAYRLTGSSDLYERSGRRPYASV
                     NFVTCHDGFTLNDLVSYNRKHNEDNLEGNRDGHDHNLSYNFGVEGPTDDPDILEQRAR
                     QRRNLLATLLLSQGVPMLCAGDEIARTQAGNNNAYCQDNEISWLDWELDEEKRDLLEF
                     TRLLIRLFHTHPVLRRRKFFQGRKIRGSEVKDLTWLRNDGREMTERDWHDSARRSLAL
                     RLAGDAIDEVDARGNRVAGDTLLILMNAAPAPARFVLPAHRPRVRWELVLDTREPTGK
                     PRRPLLLRGGSYELEPRSMALFRLQRRDEHE"
     gene            complement(3429..4778)
                     /locus_tag="AC159P_07285"
     CDS             complement(3429..4778)
                     /locus_tag="AC159P_07285"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF039907.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HIR3695294.1"
                     /translation="MNDYRNLATELVAELKKQGVDQSDVFITAGSEFSSDIRLGKIEK
                     LEQSISKGLGLRIIKNGATALTFTTDFADKSVKSLVKETMDIVKVSNADQYNGLAPKE
                     YLGAYAGKLALFDESLAAMTPEKKLAMAREMEEAGMKADKRITNTEGSSWYDATRQLT
                     LANSDGFHGQYKTTVASLSISLLAEENGVKQTDYWYSFNRFVKNLESPAKLGQEAARR
                     VVAKLGGRKVKSQKVPVVLDPMISRGFISMVFNGASGRSIWRKASFLLDKVGQEIASP
                     LVTIMDDATMWDGPSSRPFDGEGVKSSQVTVVDKGVLKSYICDSYSARRLNLKPTGHA
                     ARSYQSLPGIGSTNLYLKNGTTDPKEIVKSVKSGLYVTGLFGQGVNMVTGDISRGAVG
                     FWIENGEISYPVQEITLAGNALTLLKNIAAVGNDLNFRFGGTAAPTILLSEATVGGA"
     gene            complement(4809..6344)
                     /locus_tag="AC159P_07290"
     CDS             complement(4809..6344)
                     /locus_tag="AC159P_07290"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011525556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HIR3695295.1"
                     /translation="MKRRDFMLKGSLAAAAVATSDLWVSRIVAGEKATNTLPILDTYF
                     NVGKDDMDKLLAAALSKGADFADLFFEYRISSSLQFEEDQVKSARRGIVSGVGIRAIK
                     GDQVGYAFSEDMTLEKMKEAALSAAAIANDKLARVKSVGVSEIRPKNLYPVADLVTSA
                     DLGKKLTLIKEANEAAKGYSPKVIRVQVFFDDEVKYLAYANSEGRMWQDMQPVFTFTT
                     GCIAEEGKNRQQSYETGGGRIGLEYFDKKRNASHIAKEAARIAVQNLSAEEAVAGPQT
                     IVLGPADSAVLLHEAVGHGLEADFNWKKLSNYSNRVGERVASEHCTVIDEGLFENMRG
                     TINVDDEGNAPTSTMLIEKGILRGYMCDRISAKQLGVKPTGNGRRQAYNYAPMARMTN
                     TYMLPGKYDPEEIIKSTKSGIYAKRFAGGQVDITKGDFTFTVTESFRIEDGKLTAPLK
                     DVTLIGNGPDVMTKVTMVGSDLKFADGGWTCGKNGQSVPVGMGIPTTKVSEITVGGTK
                     VKA"
     gene            complement(6579..>6919)
                     /locus_tag="AC159P_07295"
     CDS             complement(6579..>6919)
                     /locus_tag="AC159P_07295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695296.1"
                     /translation="RATLDDMRERGGYDVSRLTRITLSDDGSFEIVDMPDWWNNGFGK
                     SYRGFASFSGRWALSNIGGHWRIGLSFFGTRRFLNLVEHKMGGSPPYCVEIIVGDPDS
                     GDEMIFVRKE"
BASE COUNT         1172 a         2238 c         2181 g         1328 t
ORIGIN      
        1 gcgaaccggc cccgcccgta tggagttatt gaagagatat tggttcgagt tcgatatgtc
       61 cggactgtcc gacgagcaac tccggcgata cccttacttt cctgcttatg gagtgggagt
      121 gacggctttc gactacgagg atgctttacg gttgatacgg aagtgggttc tccaagatcg
      181 ttatgacatg cctgaaatcg ccaaagtcac ggagggcgtc gatgtctcgc agctactgga
      241 gcggcccttc gcacgcaccc gcttgttgcc acatttgggc tgtcccgtat ggagaggcgt
      301 ttggcatccc gccttcaatc tttggtacgg agcggacacg gagctttgat tgttattttg
      361 cccgacatgg aaaacgatcc actgccgccc cgccgcgcaa cttaaactga accgccatcc
      421 ggtattataa tttcgccaca catcacgaca aggagcaacc cgccccgaac gggttcgaca
      481 gggtgaccgg atggattggc aggagaacat catcgaggcg ggcggcggga tcaagcacct
      541 gctcgccgag accaggcgca tcgccgtgct cggcatcaag accgagcagc aatcgtacca
      601 gcccgccttc tacgtgccgc agtacatggc caacgccggc ttcgaggtca tccccgtgcc
      661 ggtctactac ccggaggtga cgcgcatcct cgggcgcgag gtctaccgca aactggtgga
      721 cgtgcccggc gaggtcgatc tcgtcaacgt cttccgccgc ccgcaggaca tcgaggcgca
      781 cgtcgaggac atcctcgcca agcgcccgcg cgccgtctgg ttccagtccg gcatccgcca
      841 cgacgcggcg gcggagcgct tcgcccgcgc cggcatcaag gtcgtgcagg atcgctgcct
      901 catggtcgag caccgcatgg ccgcccgctg aaaagatcga gggaaagaga ctgaggggaa
      961 aaggggaaga ggtgaaaggg cgaaaaggga agatgagaag cagcgtgcct ctcatcttcc
     1021 ttcttcccca ttttaccttt tcgcctctcc ccttttcccc cttcttcctt ttcgcccttt
     1081 cacctcttcc ccttttcccc caaggagtct ttgatgaagg tctggcccgg acaaccttac
     1141 ccgctcggcg cgacgtggga cggcgcgggc gtcaacttcg cgctcttctc cgagaacgcg
     1201 accgccgtcg agctgtgcct cttcgactcg gcggacgccg tgcaagagtc gcggcgcgtt
     1261 cgcctgaccg agcacacgga tcaggtctgg cacgcgtacc tgccggaggc gcggcccggc
     1321 ctgctctacg gctaccgcgt ccgcggcccg cacgaaccgc gcgccggcca ccgcttcaac
     1381 ccgcgcaaac tcttgatcga cccctacgcg aaggccatct ccggcaccgt ccgctgggac
     1441 gaggcggtgt tcggctatac catcggccac gagctgcgcg acctctcgct cgacacgcgc
     1501 gacagcgccg cgcacgtccc acgctgcgtc gtcgtcgata actccttcga ctggggcgac
     1561 gacacgcgcc cgcgcacgcc ctggcaccgc acgctcatct acgagctgca cgtcaagggc
     1621 ttcaccatgc gccaccccga ggtgcccgaa gagctgcgcg gcacctacct cgggctggcg
     1681 cacccttcgg tgatcgacta tctcaaatcg ctcggcgtca ccgccgtcga gctgatgccc
     1741 gttcaccagt tcatctccga caagtttctc gttgaccgcg ggctgacgaa ttactggggc
     1801 tacaactcca tcggcttctt cgcgcccgac gtgcgctact cggcgggcgg ggcgctgggc
     1861 gcgcaggtct cggagttcaa gacgatggtc cgcacgctcc accgcgaagg catcgaggtc
     1921 atcctcgacg tggtctacaa ccacacgggc gagggcagcc acatggggcc gacgctctcg
     1981 ttccgcggga tcgacaacgc ggcctactac cgcctcgcgc acgcagaccg cagccgctac
     2041 gtcgattaca ccggctgcgg caacacgctc aacatgaccc acccgcgcac gctgcaaatc
     2101 atcatggaca gcttgcgcta ctgggtgcag gagatgcgcg tggacggctt ccgcttcgat
     2161 ctggccgccg cgcttgcgcg cgagctgcac gaggttgacc gcctcggcgc cttcttcgac
     2221 atcatccatc aagaccccgt gctctcgcag gtcaagctca tcgccgagcc gtgggacttg
     2281 ggcgagggcg gctatcaggt cggcaacttc cccgtcctgt gggccgagtg gaatggcgtc
     2341 taccgcgacg ccgtgcgcgg ctactggcgc ggcgactcag ggctgatcga cgacctcgcc
     2401 taccgcctga cggggagcag cgacctctac gagcgcagcg ggcgcagacc ttacgccagc
     2461 gtcaacttcg tcacctgcca cgacggcttc accctgaacg atctggtgag ctacaaccgc
     2521 aagcacaacg aggacaacct cgaaggcaac cgcgacggcc acgaccacaa cctcagctac
     2581 aacttcggcg tcgaggggcc gaccgacgat cccgacatcc ttgagcagcg cgcgcgccag
     2641 cgacggaacc tgctcgccac actgctcctc tcgcagggcg tgccgatgct ctgcgcgggc
     2701 gacgagatcg cgcgcacgca ggcgggcaac aacaacgcct actgtcagga caacgaaatc
     2761 tcctggctcg actgggagct ggacgaagag aagcgcgacc tgcttgagtt cacgcggctg
     2821 ctcatccgtc tcttccacac gcaccccgtc ctgcgccgcc gcaaattctt tcaggggcgc
     2881 aagattcgcg gctccgaggt caaagacctg acgtggctcc gcaacgacgg gcgcgagatg
     2941 accgagcgcg actggcacga ctcggcgcgc cgctctctgg ccttgcgcct cgccggcgac
     3001 gccatcgacg aggtggacgc gcgcggcaac cgcgtcgccg gcgacacgct cctcatcctg
     3061 atgaacgccg cgcccgcgcc cgcgcgcttc gtcctgcccg cacaccgccc gcgcgtccgc
     3121 tgggaactcg tcctcgacac gcgcgagccg accggcaagc cccgccgccc gctcctcctg
     3181 cgcggcggct cctacgaact cgaaccccgc tcgatggccc tcttccgctt acaacgtcga
     3241 gacgaacacg agtgagcagt aggcagtagg cagtaggcgg taggcagtta agacaaaaga
     3301 acagtgaaga tgcttgggag gtaaagtgcc aagcatcctc gttcgtcaag cagaaagagc
     3361 aagaggcttg agacgtagcg tctcaagcct cttgctttaa ctgcctactg cctactgcct
     3421 actgtttctc acgcgccgcc gaccgtggcc tcggagagga ggatggtcgg ggcggcggtg
     3481 ccgccgaagc ggaagttgag gtcgttgccg accgccgcga tgttcttcag cagcgtgagg
     3541 gcgttgcccg cgagcgtgat ctcctgcacg ggatagctga tctcgccgtt ctcgatccag
     3601 aagccgaccg ccccgcgcga gatgtcgccc gtcaccatgt tcacgccctg cccgaagagg
     3661 cccgtcacgt agaggccgct cttgacgctc ttgacgatct ccttcgggtc ggtcgtgccg
     3721 ttcttcagat agaggttggt cgagccgatg ccgggcagag actggtagct gcgcgcggcg
     3781 tggcccgtcg gcttgaggtt caggcggcgc gccgagtacg agtcgcagat gtaagacttg
     3841 agcacgccct tgtccacgac cgtcacctgc gaggacttga cgccctcgcc gtcgaacggg
     3901 cgcgaggagg ggccgtccca catcgtcgcg tcgtccatga tggtgacgag cggcgaagcg
     3961 atctcctgcc cgaccttgtc cagcaggaac gaagccttgc gccagatgct ccggcccgag
     4021 gcgccgttga agaccatcga gatgaagccg cgcgagatca tcgggtcgag gacgaccggc
     4081 accttctgcg actttacttt ccgcccgccg agcttggcga cgacgcgccg cgccgcctcc
     4141 tgcccgagct tcgcgggcga ttcgaggttc ttgacgaagc ggttgaaaga gtaccagtag
     4201 tcggtctgct tgacgccgtt ctcctcggcg aggagcgaga tcgagaggct ggccacggtc
     4261 gtcttgtact gtccgtggaa gccgtccgag ttggcgagcg tgagctggcg ggtggcgtcg
     4321 taccagctcg acccctcggt gttggtgatg cgcttgtcgg ccttcatccc ggcctcttcc
     4381 atctcgcgcg ccatcgcgag cttcttctcc ggcgtcatcg ccgccagaga ctcgtcgaaa
     4441 agcgcgagct tgccggcgta ggcgccgagg tactccttcg gcgcgaggcc gttgtactgg
     4501 tctgcgttcg agaccttgac gatgtccatc gtctctttga cgaggctctt gacggacttg
     4561 tcggcgaagt cggtggtgaa ggtcagggcg gtcgcgccgt tcttgatgat gcgcaggccc
     4621 aagcccttgc tgatggactg ttcgagtttt tcgatcttgc cgaggcggat gtcggacgag
     4681 aactcggagc cggcggtgat gaacacgtcg ctctggtcaa cgccctgctt cttcagctcg
     4741 gcgacgagtt cggtggcgag attgcggtaa tcgttcatga cggtctcttc tccttcgtcg
     4801 tctccgagtt aagccttgac cttcgtgccg ccgacggtga tctccgagac cttcgtcgtc
     4861 gggatgccca tgccgaccgg caccgattgg ccgttcttgc cgcacgtcca gccgccgtcg
     4921 gcgaacttca ggtcgctccc gaccatcgtc accttcgtca tcacgtcggg gccgttgccg
     4981 atcagcgtca cgtccttcag cggggcggtc agcttgccgt cctcgatgcg gaagctctcg
     5041 gtgacggtga aggtgaagtc gcctttggtg atgtccacct gcccgcccgc gaagcgcttg
     5101 gcgtagatgc cgctcttggt ggacttgatg atctcctcgg ggtcgtactt gccggggagc
     5161 atgtaggtgt tggtcatacg cgccatcggg gcgtagttgt aggcctggcg ccgcccgttg
     5221 ccggtcggct tgacgccgag ctgcttggcg ctgatgcggt cgcacatgta gccgcgcagg
     5281 atgcccttct cgatgagcat ggtcgaggtc ggcgcgttgc cctcgtcgtc cacgttgatg
     5341 gtgccgcgca tgttctcgaa caatccttcg tcgatgacgg tgcagtgttc ggaggcgacg
     5401 cgctcgccga cgcggttcga gtagttcgag agcttcttcc agttgaagtc ggcttcgagg
     5461 ccgtggccga ccgcctcgtg caggaggacg gcggagtccg ccgggccgag gacgatggtc
     5521 tgcgggcctg cgacggcctc ttcggctgaa aggttctgga cggcgatgcg cgccgcctcc
     5581 ttggcgatgt gcgaggcgtt gcgcttcttg tcgaagtatt cgaggccgat gcgcccgccg
     5641 cccgtctcat aagactgctg gcggttcttg ccttcctcgg cgatgcagcc ggtggtgaag
     5701 gtgaagacgg gctgcatgtc ctgccacatc cggccctcgc tgttggcgta ggcgagatac
     5761 ttcacctcgt cgtcgaaaaa gacctgcacg cggatgacct tcgggctgta gcccttggcg
     5821 gcctcgttcg cctccttgat gagcgtgagc tttttgccga ggtcggcgct ggtgacgagg
     5881 tctgcgacgg ggtagaggtt cttcgggcgg atttcgctga cgccgacgct cttcacgcgg
     5941 gcgagcttgt cgttggcgat ggcggcggcg gacagagcgg cctccttcat cttctccagc
     6001 gtcatgtctt cggagaaggc gtagccgacc tgatcgccct tgatggcgcg gatgccgacg
     6061 ccggagacga tgccgcggcg cgcgctcttg acctgatcct cctcgaactg gagcgaggac
     6121 gagatgcggt actcgaagaa gaggtcggcg aagtcggcgc ccttcgagag ggcggcggcc
     6181 agcagcttgt ccatgtcgtc tttgccgacg ttgaaatagg tgtcgaggat ggggagcgtg
     6241 ttggtggcct tctcgccggc gacgatccgg ctgacccaca ggtcgctggt ggcgacggcg
     6301 gctgcggcga gcgagccttt caacatgaag tcgcgtcttt tcatcttcat tttccttccg
     6361 ggggaacact tgcgtcgccc caagtctggc ggtgaagcgg atttagttct ggagtgcccg
     6421 aatgagcttc gacaacagtt tgcgcgcggg acgggcgctt gtaccttacg caaagcgccc
     6481 gattatgttt caggccggga cggaagggaa catttatgtg atgaggcgtc gggattcagg
     6541 gacgagttga acgaaaacga ggacgaacgt tcggggggct actccttcct gacgaagatc
     6601 atttcatcgc cggaatcggg atcgcccacg ataatctcga cgcagtacgg cggactgccg
     6661 cccattttat gctcgaccag gttgaggaag cgacgtgtgc cgaaaaacga gaggccgatc
     6721 cgccagtgtc ctccgatgtt ggataacgcc caccgcccgg agaaggaagc aaagcctctg
     6781 tatgatttgc cgaagccgtt gttccaccag tccggcatgt cgactatctc gaagcttccg
     6841 tcgtcactca gtgttatccg ggtgagcctg gagacatcgt acccgccgcg ttcgcgcatg
     6901 tcgtcgaggg tcgcccggt
//
LOCUS       DBNGOH010000368         3182 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2621319, whole genome shotgun sequence.
ACCESSION   DBNGOH010000368 DBNGOH010000000
VERSION     DBNGOH010000368.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3182)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3182)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3182
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2621319"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            115..1560
                     /locus_tag="AC159P_07300"
     CDS             115..1560
                     /locus_tag="AC159P_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020601558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6986 family protein"
                     /protein_id="HIR3695297.1"
                     /translation="MKTSLPADLLRQTAERLQTANLDFAARYPGESGRRQPVHTVYGG
                     AHLFKSDTAQRLGALSLRSLEQYAPDFVAFAKAVGLAGAEELPDALDENATFRTLLES
                     NPEAVRRDHRPAWLAHTVHARVAEKLRREPVEDFRIDFEDGYGNRPDAEEDAHASQAA
                     RETAAAMKAATLPPFLGIRIKPFNEELRRRSTRTLDIFLSTLVEAAGGTLPPNFYVTL
                     PKVTIPEQVAALADIFDALEPALGLASGTLKMELMVETTQSIIDRHGRSNLPLLLEAA
                     RGRMTGAHFGTYDYTASCQITAAHQHMLHPSCDFAKHMMQVAYGGTGVWLSDGATNIM
                     PVPVHRAAEGAELSQEQLDENRASVHRAWRLHYEHVRHSLTGAFYQGWDLHPAQLPTR
                     YAAVYCFFLESLEAASERLRNFIDKAAKATLVGDVFDDAATGQGLLNYFLRALNCGAL
                     TEQEALELSGLTIDELRSGSFVKILNGRQSL"
     gene            complement(1680..2489)
                     /locus_tag="AC159P_07305"
     CDS             complement(1680..2489)
                     /locus_tag="AC159P_07305"
                     /inference="COORDINATES: protein motif:HMM:NF017945.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate hydroxylase PuuD"
                     /protein_id="HIR3695298.1"
                     /translation="MDIPPYVSEWLNLLVRWAHVFAGILWIGTTYFFTWLDGRFTERE
                     GEGGKRQVWMVHSGGFYVVDKTKELDLDQHLHWFRFEALLTWASGFVLLVLVYYLGGL
                     MVDTDVLDISNSTAVLLGLGVILVSGFVYDVLLRTPLARNDTLFAAVAFLLVVGLSYA
                     LTHVFSGRAAYIHLGAVFGTIMVVNVWMHILPAQRKMIAALKEGKTPDERLAAQAKLR
                     SKHNTYMVIPVVFTMIGNHFPVSTYGHQYNWLILSALVLAGWFAAKIIRRA"
     gene            complement(2614..2970)
                     /gene="uraH"
                     /locus_tag="AC159P_07310"
     CDS             complement(2614..2970)
                     /gene="uraH"
                     /locus_tag="AC159P_07310"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033971 - hydroxyisourate hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006144 - purine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HIR3695299.1"
                     /translation="MSAISTHVLDTSQGRPAGGVPVTLEIGSARDDGFYTWEPHGGGE
                     TDADGRVKNLLPAGAALAAATYRLTFDTGAYFSARGVESFYPSVTIVFRIRDASQHHH
                     VPLLLSPYGYSTYRGS"
     gene            complement(2987..>3182)
                     /locus_tag="AC159P_07315"
     CDS             complement(2987..>3182)
                     /locus_tag="AC159P_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002632113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline
                     decarboxylase"
                     /protein_id="HIR3695300.1"
                     /translation="AAANRAYEQRFGFIYIVCATGKSAGEMLSILRARLGHDEARELR
                     VAAEEQRKITRLRLEKLLDA"
BASE COUNT          580 a         1103 c          983 g          516 t
ORIGIN      
        1 gccccgcaca ctcctcttca cccctttacc tttccctatc ccccgcgctt cgttctagaa
       61 tgccccgtgc ccgcgccgct ttcgacgaca tctttcgcac cctagaggag ccacatgaag
      121 acttcccttc ccgcagacct tttgcgccag accgccgagc gactccagac cgccaacctc
      181 gacttcgccg cacgctaccc cggcgaatcg ggccgccgcc agcccgtcca caccgtctac
      241 ggcggcgcgc acctcttcaa gtccgacacg gcgcagaggc tcggcgcgct cagccttcgc
      301 tcgctcgaac agtacgcgcc cgacttcgtc gccttcgcca aggccgtcgg cctcgcgggc
      361 gccgaagagc tgcccgacgc gctcgacgag aacgccacct tccgcacgct cctcgaatcg
      421 aaccccgagg ccgtccgccg cgaccaccgc cccgcctggc tcgcgcacac cgtccacgcg
      481 cgcgtcgcgg agaagttaag acgcgagccg gtcgaggact tccgcatcga cttcgaagac
      541 ggctacggca accgccccga cgccgaagag gacgcgcacg cctcgcaggc ggcgcgcgaa
      601 acggctgccg cgatgaaggc cgccacgctc ccgcccttcc tcggcatccg catcaagccc
      661 ttcaacgaag agctgcgccg gcgcagcacg cgcacgctcg acatcttcct ctccacgctc
      721 gtcgaggccg cgggcggcac gcttcccccg aacttctacg tcacgctccc caaagtcacc
      781 atccccgaac aggtcgcggc cctcgccgac atcttcgacg cgctcgaacc agccctcggc
      841 ttagcctccg gcaccctcaa gatggagctg atggtcgaga ccacgcagtc catcatcgac
      901 cgccacgggc gcagcaatct cccgctgctg ctcgaggccg cgcgcgggcg catgacgggc
      961 gcccacttcg gcacctacga ctacaccgcg agttgccaga tcaccgccgc ccaccagcac
     1021 atgctccacc cctcctgcga cttcgccaag cacatgatgc aggtcgccta cggcggcacc
     1081 ggcgtctggc tctccgacgg cgccaccaac atcatgcccg tccccgtcca ccgcgccgcc
     1141 gaaggcgctg agctatcaca ggaacaactc gacgagaacc gcgcctccgt ccaccgcgcc
     1201 tggcgcctcc actacgaaca cgtccgccac tcgctcaccg gcgccttcta ccagggctgg
     1261 gacctccacc ccgcacagct ccccacgcgc tacgccgccg tctactgctt cttcctcgaa
     1321 agcctcgaag cggcctccga acgcctccgc aacttcatcg acaaagcggc gaaggcgaca
     1381 ctcgtcggcg atgtcttcga cgacgccgcc accggtcagg gtctcctcaa ctatttcttg
     1441 cgcgccctca actgcggcgc cctcaccgaa caggaagcgc ttgagctttc cggcttgacc
     1501 attgacgagc tgcgctccgg ctcgttcgtc aagattctga atgggcggca gagtttgtaa
     1561 gaaggaacaa tcgatggaaa cgctcacatg cgatagggcg cgagtattac tcaaggaaac
     1621 gccccctaca gttgaaaaat caggggtaaa atcgccagca cagaagacga gagggcaaat
     1681 cacgcccgcc ggatgatttt agccgcgaac cagcccgcga ggacgagcgc ggagaggatg
     1741 agccagttgt actggtggcc gtaggtggag acggggaagt ggttgccgat catggtgaag
     1801 acgacgggga tgaccatgta ggtgttgtgc ttggagcgga gcttggcctg ggcggcgagg
     1861 cgttcgtcgg gcgtcttccc ttccttgagc gcggcgatca tcttgcgctg ggcaggcagg
     1921 atgtgcatcc agacgttgac gaccatgatg gtgccgaaga cggcgccgag gtggatgtag
     1981 gcggcgcgtc cgctgaagac gtgcgtgagg gcgtaggaga ggccgacgac gagcaggaag
     2041 gcgaccgccg cgaagagcgt gtcgttacgc gcgagcggcg tgcggagcag cacgtcgtag
     2101 acgaaccccg agacgaggat gacgccgagg ccgaggagga cagcggtgct gttgctaatg
     2161 tcgagtacgt ccgtgtccac catcaggccg ccgaggtagt agacgaggac gagcaggacg
     2221 aagcccgaag cccaagtcag cagcgcctcg aagcggaacc agtggaggtg ctgatcgagg
     2281 tccagctcct tcgtcttatc gacgacgtag aagccgccgc tgtgcaccat ccacacctgc
     2341 cgcttgccgc cctcgccctc gcgctccgtg aacctcccgt cgagccacgt gaagaaatag
     2401 gtcgtgccga tccacaggat gccggcgaag acgtgcgccc agcgcacgag caggttgagc
     2461 cattcgctca cgtacggggg aatgtccatc aaggtctctc cattggggat tgtggattgc
     2521 ggaatgcgga ttcatcgtcg atgaaagcac cgccgccgtc aatcatcttc gcttcttcaa
     2581 cccgcaatcc gaaatccgca ttccgcaatc gtttcagctt ccgcggtagg tcgagtagcc
     2641 gtaggggctg agcaggagcg ggacgtggtg gtgctgcgag gcgtcgcgta tccgaaagac
     2701 gatcgtgacc gaggggtaga agctctcgac gccgcgcgcg gagaaatagg cgccggtgtc
     2761 gaaggtgaga cggtaggtgg cggcggcgag ggctgcgccc gcgggcagca ggttcttgac
     2821 ccgaccgtcg gcgtccgtct cgcctccgcc gtgcggctcc cacgtgtaga agccgtcgtc
     2881 gcgggcgctg ccgatttcca gtgtgacggg gacgccgccc gcggggcggc cctgcgaggt
     2941 atcgaggacg tgcgtgctga tggcgctcat gcgttcggta gctcgctcat gcgtcgagta
     3001 gtttttcgag tcggaggcgc gtgatcttgc gctgctcttc ggcggcgacg cgcagctcgc
     3061 gcgcttcgtc gtggccgagt cgtgcgcgca ggatcgagag catctcgccc gcgctcttac
     3121 cggtggcgca gacgatgtag atgaagccga agcgttgttc gtatgcgcgg ttggccgcgg
     3181 cg
//
LOCUS       DBNGOH010000369         7470 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3171124, whole genome shotgun sequence.
ACCESSION   DBNGOH010000369 DBNGOH010000000
VERSION     DBNGOH010000369.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7470)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7470)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7470
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3171124"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1069)
                     /locus_tag="AC159P_07320"
     CDS             complement(<1..1069)
                     /locus_tag="AC159P_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020714757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate epimerase family protein"
                     /protein_id="HIR3695301.1"
                     /translation="MSDALKLEKYSFGVGDRFAHQAKAQLQACMRAAAEGVDVAPVWN
                     KSYREHSIVGSQPASVLAAARAAVSELDWTKPYHLDADHIRLETVDSFIEPCDFYTLD
                     VADAIGQPASADDVKAFVERHPELVGRIEIPGIEQPFETDRAEIERIANKFLAAVQEA
                     GRLYRHIAAAKGEGKFITEVSMDETDTPQTPPELLVILAAIADEGIPAQTIAPKFTGR
                     FNKGVDYVGDVAQFEKEFNEDLAVIAHAVKQYGLPDNLKLSVHSGSDKFSIYEPIRRA
                     LERTGAGIHVKTAGTNWLEELIGLAESGGDGLKMAQEIYGKALDDIDALSAPYATVID
                     IDRAKLPSKEEVNGWSSDEFVS"
     gene            complement(1190..2128)
                     /locus_tag="AC159P_07325"
     CDS             complement(1190..2128)
                     /locus_tag="AC159P_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015267778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695302.1"
                     /translation="MSKMQTETLTRRAAGDNVWLSHAADEQSSLDALARLDAHDIRPE
                     VLDTEGAVGEAMLAELDRLARDKEGDITMILLGGRGAQALHRLLGERAKKGGDALLPR
                     LRVFTQDALAPMRMASSLSFVRDFERLLGHEFFEQVKSFTSMKTDTADLEGELTAYVR
                     ELEAAGGADIFFLGHGPEADDASHLAYIKPGSGATAADAAGLIPISSSILEHHISKFK
                     AGGSAVGEEDEAECRRATHILTLGPAAILGARRIVQSVVDASTAPAKKRTYRRVLETR
                     IADDPRERARQLDANPGLWVRLHPGVRSLILPDVLG"
     gene            complement(2243..6295)
                     /locus_tag="AC159P_07330"
     CDS             complement(2243..6295)
                     /locus_tag="AC159P_07330"
                     /inference="COORDINATES: protein motif:HMM:NF051698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HIR3695303.1"
                     /translation="MRPVRILLVLSLLAVAAFGQTNKGGISGTVLDSNGAAVTGATVI
                     ITNIGTGQQQTLTSSESGAFSATSLDPVTYSVSVEMAGFKKAVLESVKVDTATTVPVN
                     VILETGAVTDEVTVVADAPLLNTESGTTTSTVTERQIQEVPLSNRSVLDLALTAPNVS
                     GDAGSEDPEVTSGQPVPGFNLSLNGGRPGSTAILADGVNNTGVGIARSVVSFTPETVQ
                     EFTVQTSAYSAEYGNTGGGIINATTKSGTNNFNGVALWYHRNPKTAASPYRIGTGPRP
                     PGNLRYNQVSISAGGPVYLPRFGEGGPTWYDGHDKTFFFFAYEPRWRRDFLTVSTLVP
                     TAAQRAGDFRNLVRTASGFVPADVAARFPVQGASIGPADIYRQFNLVGGRLVPIALGT
                     GQTFPQFSSNGQLNVIPAEFLDPTAQNLLQFLPAAGDYFIDDAGLIRNQTIQRFVQQD
                     ETRYTLRLDHNINNNQKVNFRYTVTPAVGVRGFASEVNGNSAAYSDAKQLLLAFNSIL
                     SPTIVNDLRLNYTRGVFSEDFSPEFSINGGRNLGNELGLPSLTSGGIPLFQYDADQGG
                     NAFSNIGSSGSTNNFNVEERFNINDIVYMTRGDMTWKMGVDLSHARLNVVPFFAASGG
                     RWTFRSFQTNINRSTGNQNSVGGNSFASLLIGVPDRVDVRPLLLDYNYRWNSGALFVQ
                     NDWKVRPNLTLNLGLRYSLQYPRYEKNNLQGVFRPDLAIPTALTEAQRRATATGLGVA
                     ATAPIPDFVPTSVVIPPFAFSGLGGRSKYITPVDYWGLEPRFGFAWSPKLKVLGLDLE
                     KRSAVIRGGYGLSHAPLTGNNRSPNPDFGQFITIVNTATGSPAGNSQDAGQPLRLTGN
                     DPVIAGRPLFEQLGVTSDGLVTTNSIAIPAVADTGFSTGSGKVPYSQNWNLTLSFEPF
                     GNTVVEFAYVGNKGTHLYMPLVNLNPRDIEFVEFLEGQNLNAETAFNDPLGRRNLAGG
                     LITIQRSSVQTPFFGFNNLNRFYDPSANSIRHATYVDVRRRVARGFTFTANYTFAKSI
                     DDASDASPDTRVITEVGQTQGHVSYGLPRSVDRSVSTFDIKHNFSSTFVLDFPFGRGR
                     QFLKDAPGVVDAAFGGWTMSGVFRLQGGVPFLPYLTDTNRLGGTNRTIRPNIIEGVPL
                     VNPLWSEDCTLGATCEPYVNPSAFIRPPKGELGNAPRTIDSLRAPIQEYFDLSFQKNF
                     RWPFADNEGKRRINFRVDLINAFNHPVFRLGSSGSAGFTTAVPSEAVLTQAELNTWLA
                     ANPGRTATLTQVNNLIINSRLSTGALPLDFFSVPVPQGFATSTPQSFDITTLEGLKLF
                     RLRQSYDANFGSLRVVPNARYIQFGLRIFF"
     gene            complement(6933..>7470)
                     /locus_tag="AC159P_07335"
     CDS             complement(6933..>7470)
                     /locus_tag="AC159P_07335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HIR3695304.1"
                     /translation="PVDISNGWRVTFGQGVTKQMDRLRSWTEDEDTRYFSGTATYERE
                     VTVPESMLRAGAPVILHFGEGKPLPPQNLRAGMQAWLEGPVRDAALVYVNERRAGSVW
                     MPPWSLDVSGLLRPGANRLRIVVANTALNHMAGRRLPDYRLLNLRYGERFTPQDMDKV
                     RPEVSGLLGPVRLVAGDR"
BASE COUNT         1178 a         2330 c         2638 g         1324 t
ORIGIN      
        1 cggagacgaa ctcgtcggac gaccacccgt tcacctcttc cttcgagggg agcttcgcgc
       61 ggtcgatgtc gatgacggtg gcgtagggcg cgctcaaggc gtcgatgtcg tcgagcgcct
      121 tgccgtaaat ctcctgcgcc atcttgaggc cgtcgccgcc cgactcggcg aggccgatca
      181 gctcttcgag ccagttggtg ccggcggtct tgacgtggat gccggcgccg gtgcgttcga
      241 gggcgcggcg gatcggctcg tagatggaga acttgtcgct gccggagtgg acggagagct
      301 tgaggttgtc gggcaggccg tactgtttga cggcgtgggc gatgaccgcg aggtcttcgt
      361 tgaactcctt ctcgaactgc gcgacatcgc cgacgtaatc gacgcccttg ttgaagcggc
      421 ccgtgaactt cggcgcgatg gtctgcgccg ggatgccctc gtcggcgatg gcggcgagga
      481 tgaccaaaag ctcgggcggc gtctgcggcg tgtcggtctc gtccatcgag acctcggtga
      541 tgaacttgcc ctcgcccttc gcggcggcga tgtggcggta gaggcggccc gcctcctgca
      601 cggcggcgag gaacttgttg gcgatgcgct cgatctcggc ccggtcggtc tcgaacggct
      661 gctcgatgcc cggaatctcg atgcgtccga ccagctcggg gtggcgttcg acgaaagcct
      721 tcacgtcgtc cgcggaagcg ggctggccga tggcgtcggc cacgtcgagc gtgtagaagt
      781 cgcacggctc gatgaacgag tccacggttt cgagccggat gtggtcggcg tcgaggtggt
      841 agggcttcgt ccagtcgagt tcgctgaccg ccgcccgcgc cgcggcgagc acgctcgcag
      901 gctgcgagcc gacgatggag tgctcgcggt acgacttgtt ccacacgggc gcgacatcca
      961 cgccctccgc cgccgcccgc atgcacgcct gaagctgcgc cttcgcctga tgcgcgaagc
     1021 ggtcgccgac gccgaacgaa tatttttcca gtttaagtgc gtcgctcatg gtctacctcc
     1081 gagaaaaatg atggacgatg aatgcggaat gatgaataag aatcagcttg aatgatgacg
     1141 ggcaccgtgc ttcttattca tcatccatca ttccgcattc gtcatttctt cagccgagta
     1201 cgtccggcag tatcaaagac cgcacgccgg ggtgcaggcg cacccagagg ccggggttcg
     1261 cgtcgagctg gcgggcgcgc tcgcgcgggt cgtccgcgat gcgggtttcg agcacgcggc
     1321 ggtaagtccg cttcttggcc ggcgcggtcg aggcatcgac gacggattgc acgatgcgcc
     1381 gcgcgccgag gatggccgcg gggccgagcg tcaggatgtg cgtggcgcgg cggcactcgg
     1441 cctcgtcctc ttcgcccacg gcggagccgc ccgccttgaa cttgctgatg tggtgttcga
     1501 ggatgctcga agagatgggg atcagcccgg cggcgtccgc ggcggtcgcg cccgagccgg
     1561 gcttgatgta ggcgaggtgc gaggcgtcgt ccgcctcggg gccgtggccg aggaagaaga
     1621 tgtcggcgcc gcccgccgct tcgagttcgc ggacgtaggc cgtcagctcg ccttcgaggt
     1681 cggcggtgtc ggtcttcatc gaggtgaagc tcttgacctg ctcgaagaac tcgtggccga
     1741 gcagccgctc gaagtcgcgg acgaaggaga ggctggaagc catccgcatc ggcgccagcg
     1801 cgtcctgcgt gaagacgcgc agccgcggca gcagcgcatc gccccccttc ttcgcgcgct
     1861 cgccgagcag gcggtggagc gcctgcgcgc cgcgcccgcc cagcagaatc atcgtgatgt
     1921 cgccctcctt gtcgcgcgcg agccggtcga gttcggcgag catcgcttcg ccgaccgccc
     1981 cctcggtgtc gaggacttcg ggacggatgt cgtgagcgtc gagccgcgcg agcgcgtcga
     2041 ggctcgattg ctcgtccgcc gcgtgcgaga gccagacgtt gtcgcccgcc gcgcggcgcg
     2101 tcagggtttc ggtttgcatc ttggacattt cggattttac cccttgccgt ggggcgagag
     2161 caaaaaaggg cggcggcccg ccagcgccga ccgcccttcg agttttactt ctccggtgcg
     2221 aagctcacac tccgtctccc gtctagaaga agatgcggag gccgaactgg atgtagcgcg
     2281 cgttcgggac cacccgcagc gagccgaagt tcgcgtcgta ggactgccgc aagcggaaga
     2341 gcttaagccc ttccagcgtg gtgatgtcga acgactgcgg ggtactcgtg gcgaagccct
     2401 gcggcaccgg gacgctgaag aagtcgagcg gcaaggcccc ggtggacagc cggctgttga
     2461 tgatgaggtt attcacctgc gtcagggtcg ccgtgcgccc cgggttggcc gccagccagg
     2521 tgttcaactc cgcctgggtg agcacagcct cgctcggcac cgccgtggtg aagcccgccg
     2581 agccggacga gccgaggcgg aagaccgggt ggttgaaggc gttgatcaag tcaacgcgga
     2641 agttgatgcg gcgcttccct tcgttgtccg cgaagggcca ccggaagttc ttctggaacg
     2701 aaaggtcgaa gtactcctga atgggggcac gcaggctgtc gatggtgcgc ggggcgttgc
     2761 ccagctcgcc cttgggcggg cggatgaacg ccgacgggtt gacgtaaggc tcgcacgtcg
     2821 cccccagtgt gcagtcctcg gaccagagcg ggttgacgag cggcacgcct tcgatgatgt
     2881 tgggccgaat ggtgcggttc gtgccgccga gcctgttcgt gtcggtcagg taaggcagga
     2941 acggaacccc gccctgcaaa cggaagacgc cggacatcgt ccagcccccg aacgccgcgt
     3001 caaccacccc cggcgcgtcc ttcaggaact ggcgcccgcg cccgaacggg aaatcgagga
     3061 cgaaggtgga gctgaagttg tgcttgatgt cgaaggtcga gaccgagcgg tcaacgctgc
     3121 gcggcaagcc gtaagagacg tgcccctgcg tctggccgac ctccgtgatg accctcgtgt
     3181 cagggctggc gtccgacgcg tcgtcgatcg acttggcgaa ggtgtagttg gccgtgaaag
     3241 tgaagcctcg ggccacgcgg cggcgcacgt ccacgtaggt cgcgtgccgg atgctgttgg
     3301 ccgaggggtc gtagaagcgg ttgaggttgt taaagccgaa gaacggcgtc tggacgctgc
     3361 tgcgctggat ggtgatcagg ccgccggcca ggttcctgcg gcccagcggg tcgttgaagg
     3421 ccgtctccgc gttcaggttc tgcccttcca ggaattcgac gaactcgatg tctcgcgggt
     3481 tgaggttgac gagcggcatg tagaggtgcg tccccttgtt gccgacgtag gcgaactcga
     3541 ccaccgtgtt gccgaacggc tcgaacgaca gcgtgaggtt ccagttctgg ctgtacggga
     3601 ccttcccgct gccggtcgag aagccggtgt cggcgaccgc cgggatggcg atgctgttcg
     3661 tggtgaccag cccgtccgac gtgacgccga gctgctcgaa caacggacga ccggcgatca
     3721 cgggatcgtt gcccgtcagg cggaggggct ggcccgcgtc ctgcgagttg ccggcgggcg
     3781 agccggtggc cgtattgacg atggtgatga actggccgaa gtccgggttc ggcgagcggt
     3841 tgttgccggt gagcggcgcg tgcgaaaggc cgtagccgcc gcggatgacc gccgaccttt
     3901 tctccaggtc gaggccgagg actttcaact tcggactcca cgcgaagccg aagcgcggct
     3961 ccaagcccca gtagtcaacc ggcgtgatgt acttcgagcg cccgcccagc ccggaaaaag
     4021 caaaaggcgg gatgaccacc gacgtcggca cgaagtcggg aatcggagcg gtcgccgcca
     4081 cgcccagacc cgtcgccgtg gcgcggcgct gcgcctcggt cagcgccgtc gggatcgcca
     4141 gatcggggcg gaagacgcct tgcaggttgt tcttctcgta gcgcgggtac tggagcgagt
     4201 agcggaggcc gaggttcaag gtcaggttgg ggcgcacctt ccagtcgttc tgcacgaaca
     4261 aggccccgct gttccagcgg tagttgtagt cgagcaggag cgggcgcacg tccacgcggt
     4321 cggggacgcc gatgagcagg ctggcgaagg agttcccgcc gacggagttc tggttgccgg
     4381 tcgagcggtt gatgttggtc tggaacgacc ggaacgtcca cctgccgccg gaggccgcga
     4441 agaagggcac cacgttcagc cgcgcgtggc tgaggtcaac gcccatcttc caggtcatgt
     4501 cgccgcgcgt catgtagacg atgtcgttga tgttgaaccg ctcctcgacg ttgaagttgt
     4561 tggtcgagcc ggaagagccg atgttggaga aggcgttgcc gccctggtcg gcgtcatatt
     4621 gaaagagcgg gatgccgccc gaggtcaggc tgggcagccc cagctcgttg ccgagattcc
     4681 gcccgccgtt gatcgagaac tcgggcgaga agtcctcgct gaacacgccg cgcgtgtagt
     4741 tcaggcgcag gtcgttgacg atggtcggcg agagaatgga gttgaaggcc agcagcagct
     4801 gcttggcgtc gctgtaggcg gcgctgttgc cgttcacctc gctggcgaag ccgcggacgc
     4861 cgaccgccgg cgtgaccgtg tagcggaagt tgaccttttg attgttgttg atgttgtggt
     4921 cgagccgcag cgtgtagcgc gtctcgtcct gctggacgaa gcgttgaatc gtttggttcc
     4981 ggatcaggcc ggcgtcgtcg atgaagtagt cgccagccgc cggcaggaac tgcaacagat
     5041 tctgggcggt cgggtccaga aattccgccg ggatcacgtt aagctgccca ttactggaga
     5101 actgcgggaa ggtctggccc gtgcccagcg cgatcggcac caggcggcca cccaccaggt
     5161 tgaactggcg gtagatatcc gccgggccga tggaggcacc ctgaacgggg aaccgggcgg
     5221 cgacatccgc cgggacgaag ccgctggccg tccgcacgag gttgcggaaa tcgcctgccc
     5281 gctgcgcggc ggtgggaaca agcgtggaca cagtcaggaa gtccctgcgc cagcgcggct
     5341 cgtaggcgaa gaagaagaaa gtcttgtcgt gcccgtcgta ccaggtcggg ccgccctcgc
     5401 cgaaccgcgg caggtagacg gggccgcccg ccgagatcga gacctggttg tagcgcaggt
     5461 tgccgggcgg gcgcgggccg gtgccgatgc gatacggtga cgccgcagtc ttggggttgc
     5521 ggtgatacca gagcgccacg ccgttgaagt tgttggtccc ggacttggtg gtggcgttga
     5581 tgatgccgcc gcccgtgttg ccgtactccg ccgaataggc ggaggtctgg acggtgaact
     5641 cctgcacggt ctcgggcgtg aagctgacga ccgagcgggc gatgccgacg cccgtgttgt
     5701 tgacgccgtc ggcgagaatg gccgtgctgc ccggtcgccc gccgttcaga ctcaggttga
     5761 agccggggac gggctgcccg gaggtcacct ccgggtcctc gctgccggcg tcgcccgaga
     5821 cgttcggggc cgtcagcgcc aaatcgagca cgctccggtt gctcagcggg acttcctgaa
     5881 tctggcgctc cgtcacggtc gaagtcgtcg tgcccgactc cgtgttcagg agcggcgcgt
     5941 ccgcgacgac cgtgacctcg tccgtgaccg cgccggtttc gaggatgacg ttgacgggaa
     6001 ccgtggtcgc ggtgtccacc ttcacgctct ccagcaccgc cttcttgaag cccgccattt
     6061 cgaccgagac gctgtaggtc accgggtcga gcgaggtggc ggagaaggca cccgactcgg
     6121 aactcgtgag cgtctgctgt tggcccgtgc cgatgttggt gatgatcacg gtcgcacccg
     6181 tcacggctgc gccgttcgag tcgaggaccg ttccgctgat gccgcccttg ttggtctgcc
     6241 cgaaggcagc cacagcaagg agcgaaagaa ccaagaggat tcgcaccggt cgcatgagtt
     6301 tgtctccttc tcttactggc gtttcgaaaa gacgaggcgc attcagtcag gccccgcctt
     6361 tccccgcgcg cggcgtcggc actaactggg ccggcgtgcg cccgcgtgcg gggcacgtaa
     6421 cgagtcggcg cgcacggcct ggttgccgct ccgcgcggtg acggcggtct ccgggtcggc
     6481 ccgtgggata caacttggcg gcagacctcg gcccggaagc cttcagttcg gggagattgc
     6541 tcttcgagta cagggcaacc cttgaagcgc gaacgcgcgt accggtcact tgactcgatc
     6601 agttgtgaaa cgggagcggt ggcactcacg caccggaccc tgtaacgggt cgcccgcgga
     6661 gcgcgggtcg cggcctcttc catcaagacc gccggtgccc agactgcctg tctcgaatcg
     6721 acgggccggt cacgatgtaa tcgtgcacgg cggcgacagc agcttgccgt cccgtgtcgc
     6781 gccgcacggc ggcggcgaca gctttattga ttcgagaact gcgccattgt ctcagcgcgt
     6841 cgcgtctcgc gtcttggggg atttcgccct tttagtttgc acattcggcc acggctttcg
     6901 ccgcgcgcgt cggcgtgcgc gcgaactgcc tctcagcggt cgcccgcgac cagccggacg
     6961 gggccgagca ggcccgagac ttcgggccgc accttgtcca tgtcctgcgg cgtgaagcgc
     7021 tcgccgtagc gcaggttgag caggcgatag tcgggcaacc gccgcccggc catgtggttg
     7081 agcgccgtgt tggcgacgac gatgcgcaga cggttcgcgc cgggcctgag cagccccgac
     7141 acgtcgagcg accacggcgg catccagacc gagccggcgc gccgttcgtt gacgtagaca
     7201 agcgccgcgt cgcgcacagg cccttcgagc cacgcctgca tccccgcgcg caggttctgc
     7261 ggcggcaacg gcttgccttc gccgaagtgg aggatgacgg gcgcgcccgc gcgcagcatc
     7321 gactccggca ccgtcacttc tctctcgtac gtcgccgtgc ccgagaagta acgtgtgtcc
     7381 tcgtcctccg tccacgaacg cagtctgtcc atctgcttcg tcacgccctg cccgaacgtg
     7441 acgcgccagc cgttgcttat atcaaccggc
//
LOCUS       DBNGOH010000370         3540 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3721630, whole genome shotgun sequence.
ACCESSION   DBNGOH010000370 DBNGOH010000000
VERSION     DBNGOH010000370.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3540)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3540)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3540
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3721630"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            86..1267
                     /locus_tag="AC159P_07340"
     CDS             86..1267
                     /locus_tag="AC159P_07340"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HIR3695305.1"
                     /translation="MVESTRTMRDARRIGWLLALALAATSSASAQSLFELPEGVETRW
                     ASGENPKGAKGRAAQANGGRKGAPTVAIKAGESFTLAEAAGTSGTVRRIWMTFWDRSP
                     RMLRSLRIDMFWDGAARPAVSAPLGDFFGVGLGQMAPFQSALFSNPEGRSFNCVIPMP
                     FRTGMKIVLTNESTTNLDELFYEVDYTVGDRHPASALYFHAHYRRENPTRMQQDYELL
                     PRVEGRGRYLGVNIGVIVNRKTYFNTWWGEGEVKVYLDGDRELPTLSGTGAEDYVGSA
                     WGQGSYAHLYQGSPIADEQNMRWCFYRYHIPDPVYFRRDVRVTIQQIGYLAEHSRGDI
                     TRSNRRLYRAGPGLVEMDTSKDGKFEREDDYSSCAYFYLDKAENNLPPLDPVAKRVEG
                     L"
     gene            1388..2524
                     /locus_tag="AC159P_07345"
     CDS             1388..2524
                     /locus_tag="AC159P_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007680467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HIR3695306.1"
                     /translation="MKKVARLFVVLLLCQVAVAAQGQRWSEQRAKDWYAQQPWLVGSN
                     YNPASAINQLEMWQADSFDPKRIDLELGWAEQLGMNTMRVYLHDLLWEQDAEGFKRRL
                     GEFLTIAQRHKIRPMFVLFDSVWDPNPQLGKQRAPKPGVHNSGWVQSPSTKALQDPAE
                     HARLEAYVRGVVGAFAKDERVLAWDIWNEPDNMNHGSYGELEPKNKVELVLALLPKAF
                     AWARAAGATQPLSSGVWKGDWSTPEKMTEMDRLQIELSDVITFHNYDHPSELEKRINW
                     LKRYNRPMICTEYMARGNGSFFIGSLPVAKVHNVGMINWGLVQGKSQTHLPWDSWQRA
                     YTDREPSIWFHEVFRTDGTPYIQEEVDFIKRMTGKTKAKAASGN"
     gene            2531..>3540
                     /locus_tag="AC159P_07350"
     CDS             2531..>3540
                     /locus_tag="AC159P_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HIR3695307.1"
                     /translation="MNTFRTGNSIRTRLLALLCALGLFFAQGATVFARAGGITVQVDR
                     PGAAISPTLFGLFFEDINFGADGGLYPERVKNRSFEFPEPLMGWKQITPGDSKGTLYV
                     LDTGSVKRGANSHFLRVKVDAPGKGFGVTNEGFRGIGIERGAEYTFSVRARRVDGTPA
                     ALRVELVGANGEALGETRVTGFGPRWRKHTAVVRATGTDAKARMNLLVEGGGTLDVDL
                     VSLYPKDTWQRRENGLRADLVKLLKEMQPGFLRFPGGCIVEGSHLERRYQWKTTIGDL
                     DDRSLLINRWNYEFRHRPTPDYFQTFGLGFYEYFLLSEDIGAEPLPILNCGMACQFNT
                     GEL"
BASE COUNT          645 a         1146 c         1173 g          576 t
ORIGIN      
        1 cgcgggcgcg ttcgtctacc gcttcgcgcc cgcgtccgtc acgcgcctgt cgattacgtt
       61 agcctgagaa ggacggcccg accgtgtggt cgagagcaca cgcacgatgc gcgacgcaag
      121 aagaatcggt tggttgttgg cgttggcgct tgccgcgact tcgtcggcgt ccgcgcagtc
      181 gctcttcgag ctgccggagg gggtcgagac gcgctgggcg agcggcgaga acccgaaggg
      241 cgcgaagggc cgcgcggcac aggcgaacgg cgggcgcaag ggggcgccga ccgtcgccat
      301 caaggcgggc gagagcttca cgctggccga ggccgcgggc acgagcggca cggtgcgtcg
      361 catctggatg acgttctggg atcgcagccc gcgcatgctg cgcagcctgc ggatagacat
      421 gttctgggac ggcgccgcgc gcccggcggt cagcgcgccg ctcggcgact tcttcggcgt
      481 gggcctcggg cagatggcgc ctttccagtc ggcgctcttc tcgaaccccg agggccgcag
      541 cttcaactgc gtcatcccga tgcccttccg gacggggatg aagatcgtgc tgacgaacga
      601 gagcacgacg aacctcgacg agctgttcta cgaggtggac tacaccgtcg gcgacaggca
      661 ccccgcgagc gcgctctact tccacgcgca ctaccggcgc gagaacccga cgcggatgca
      721 gcaggattac gagctgctgc cgcgggtcga aggccgcggg cgctatcttg gcgtcaacat
      781 cggcgtcatc gtcaaccgga agacctactt caacacgtgg tggggcgagg gcgaggtcaa
      841 ggtctacctc gacggcgacc gtgagctgcc gaccctgagc gggacgggcg ccgaagacta
      901 cgtcggctcc gcgtgggggc agggaagtta cgcgcacctc tatcaaggct cgcccatcgc
      961 cgacgagcag aacatgcgct ggtgttttta tcgctaccac atccccgacc ccgtctactt
     1021 ccgcagggac gtgcgcgtga ccatccagca gatcggctac ctcgccgagc acagccgcgg
     1081 cgacatcacg cgcagcaacc gccgcctcta ccgcgccggc cccggcctcg tcgagatgga
     1141 cacgtcgaag gacggcaagt tcgagcgtga ggatgactat tcgagctgcg cctatttcta
     1201 tttggataag gcggagaata atctgccccc gctcgacccc gtggctaagc gggtcgaggg
     1261 gctgtgagtt atcattcgcc gcacggggac atttacgcgg cgcgggatgt tttcgccgcg
     1321 gcgcgtctcg cgcatcagct tcggtcaacc gtcattgcca agcggaggtc ttcaacccat
     1381 gtcgtcgatg aaaaaagtcg cgcgcctgtt cgtcgtgctg ttgctgtgtc aagtggccgt
     1441 cgccgcgcag ggccagaggt ggtcggagca gagggcgaag gattggtacg cgcaacagcc
     1501 gtggctcgtc ggcagcaact acaacccggc gagcgcgatc aaccagcttg agatgtggca
     1561 ggccgattcg ttcgacccga agcgcatcga tttggagttg ggttgggccg agcagttggg
     1621 gatgaacacg atgcgtgtct atctgcacga ccttttgtgg gagcaggacg cggaaggctt
     1681 caagcggcgc ctcggcgagt tcctcaccat cgcgcagcgg cacaagatca gaccgatgtt
     1741 cgtcctcttc gattcggtct gggacccgaa cccgcagctc ggcaagcagc gcgcgcccaa
     1801 acccggcgtg cacaactccg gctgggtgca aagcccgagc acgaaggcgc tgcaagaccc
     1861 tgcggagcac gcgcggctcg aagcctacgt gcggggcgtg gtcggcgcgt tcgccaagga
     1921 cgagcgcgtg ctcgcgtggg atatctggaa cgagccggac aacatgaacc acgggagcta
     1981 cggagagctg gagccgaaga acaaggtcga gctggtgctc gcgctgttgc cgaaggcgtt
     2041 cgcgtgggcg cgcgcggcgg gcgcgacgca gccgctctct tccggcgtct ggaagggcga
     2101 ctggtcaacg cccgaaaaga tgacagagat ggatcgcttg cagatcgagc tgtcggatgt
     2161 catcaccttc cacaactacg accatccgtc cgagcttgag aagcgtatca actggctcaa
     2221 gcgctacaac cgcccgatga tctgcaccga gtacatggcg cgcggcaacg gcagcttctt
     2281 catcggctcg ctgcccgtcg caaaagtcca caacgtcggg atgattaact ggggcctcgt
     2341 acaagggaag tcgcagacgc acctgccgtg ggactcctgg cagcgcgcct acaccgaccg
     2401 cgagccgtcc atctggttcc acgaagtctt ccgcaccgac ggcacgccct acattcaaga
     2461 ggaagtggac ttcatcaagc gcatgaccgg caagacgaag gcgaaagccg cctcggggaa
     2521 ttaaaggcac atgaacacgt ttcgcaccgg caacagtatt cggacgagac tgctcgcgct
     2581 gctgtgcgcg ctcggcctct tcttcgcgca gggcgcgacc gtcttcgcgc gcgcgggcgg
     2641 catcaccgtg caggtggaca ggcccggcgc ggccatctcg ccgacgctct tcggtctctt
     2701 cttcgaggat atcaacttcg gcgccgacgg cggcctctac ccggagcgcg tgaagaaccg
     2761 ctcgttcgag tttccggagc cgctgatggg ctggaagcag atcacgcccg gcgactcgaa
     2821 gggcacgctc tacgtgctcg acaccggctc cgtcaaacgc ggggccaatt cgcacttcct
     2881 gcgcgtcaag gtggacgcgc ccggcaaggg tttcggcgtc acgaacgagg gcttccgcgg
     2941 catcggcatc gagcgcgggg ccgagtacac cttctccgtc cgcgcgcgcc gcgttgacgg
     3001 aacgcccgcg gcccttcgcg tcgaactcgt cggcgcgaac ggcgaggcgc tcggcgagac
     3061 gcgcgtgacg ggcttcgggc cgcgctggag gaagcacacc gccgtcgtcc gcgccaccgg
     3121 cactgacgcg aaggcgcgca tgaacctgct cgtcgaaggc ggcggcacgc tcgacgttga
     3181 cctcgtctcg ctctacccga aagacacctg gcagcggcgc gagaacggtc tgcgcgcaga
     3241 cctcgtcaaa ctgctgaagg agatgcagcc cggcttcctg cgcttccccg gcggctgcat
     3301 cgtcgagggc agccacctcg aacggcgtta ccagtggaag acgaccatcg gcgatctgga
     3361 cgaccggagc ctgctgatta accgctggaa ctacgaattc agacaccggc ccacgcccga
     3421 ctattttcag acgttcggcc tcggcttcta cgagtacttt ctcctgagcg aagacatcgg
     3481 cgccgagcct ttgccgattc tgaactgcgg catggcctgc cagttcaaca cgggcgagct
//
LOCUS       DBNGOH010000371         2807 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4271281, whole genome shotgun sequence.
ACCESSION   DBNGOH010000371 DBNGOH010000000
VERSION     DBNGOH010000371.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2807)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2807)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2807
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4271281"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..267
                     /locus_tag="AC159P_07355"
     CDS             <1..267
                     /locus_tag="AC159P_07355"
                     /inference="COORDINATES: protein motif:HMM:NF014950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR3695308.1"
                     /translation="EVTRGCLPTEIADPSKAVGSAPLTTGTGTLDVSRGVNCYDEVRR
                     FEIDIIRRALDQTGGHQSRAARLLGMNATTLNSKIKTYNIQLRS"
     gene            complement(329..1798)
                     /locus_tag="AC159P_07360"
     CDS             complement(329..1798)
                     /locus_tag="AC159P_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008509 - monoatomic anion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006820 - monoatomic anion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HIR3695309.1"
                     /translation="MSDTPGEARATQEEVKTDGGPSRAATAGKWAVVLACAFAIALAP
                     MPAGVTPQSWRLLAIFAATIVGSIVRPLPGAAVVLLGVTATALLGVLPIERALGGYAD
                     PVVWMVLAAFMISRGMIKTGLGRRIAFLFIRAIGSRTLGLGYALILTDVLLAMIIPST
                     GARSGGIIFPIAKSLAEAYDSRPGETARRLGAFLFPLLYQCEVIICAMFLTGQASNVI
                     IARFAKEAAGVELTYARWALGAIVPGLVSLLVIPPLLYRVNPPEIKRTPDAARLAAEE
                     LRRMGPMSLGEKMMLLVFALVATLWMTSTLHGLHYATVALVGICVLLLTGVLVWEDVL
                     AERAGFDVFIWYGGLVQLASELAKTGIVERFAQGSAAYTRGWSWAAALALLLLIYFYA
                     HYAFASITAHATAMYVPFLLVILAAGAPTYLAVLSLAYLSNLSASLTHYGTTPAPIWF
                     GAGYVKQRTWWMLGLLTSVPNILIWVTVGFLWWKLLGWW"
     gene            complement(2020..2337)
                     /gene="sugE"
                     /locus_tag="AC159P_07365"
     CDS             complement(2020..2337)
                     /gene="sugE"
                     /locus_tag="AC159P_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002716147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HIR3695310.1"
                     /translation="MAWVYLLLAGLLEVVWAVGLKYTEGFTRLWPSLWTGAAMILSML
                     LLAVAVKSLPIGTAYAVWTGIGAVGTAVLGIILFGEPRETARLLCVLLIVAGIVGLKL
                     TSR"
     gene            complement(2472..>2807)
                     /locus_tag="AC159P_07370"
     CDS             complement(2472..>2807)
                     /locus_tag="AC159P_07370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695311.1"
                     /translation="HLVAYVGHNGLMDFSLASYPKKSDAGGASDAVILACASKGYFAD
                     ALREAGARPLLWTTNLMAPEAYVLKAAVDGWVAGEAGERVRERAAKEYDAYQKCGLKA
                     AQKLFASGW"
BASE COUNT          525 a          949 c          923 g          410 t
ORIGIN      
        1 gaggtcacgc gcggctgtct gccgaccgag atcgccgacc cctcgaaggc ggtcggctcg
       61 gccccgctca cgacgggcac gggcacgctc gacgtgagcc gcggcgtcaa ctgctacgac
      121 gaggtgcgcc gcttcgagat agacatcatc cgccgcgccc tcgaccagac cggcggccac
      181 caatcccgcg ccgccagact cttaggcatg aacgccacca cacttaattc aaagatcaag
      241 acctacaaca tccagctccg ctcctgaagg ttacccttgc ttgacgcgcg aaggcgccgg
      301 ataacacccg gcgcggagtt ttttatcttc accaccagcc gagcagcttc caccagagga
      361 agccgaccgt gacccagatc aagatattcg gcaccgaggt gaggaggccg agcatccacc
      421 aggtgcgttg tttgacgtag ccggcgccga accagatggg ggccggggtg gtgccgtagt
      481 gcgtgagcga ggcgctgagg ttggagagat aggcgagcga gaggacggcc aggtaagtgg
      541 gtgccccggc ggcgaggatg acgaggagga aggggacgta catcgctgtg gcgtgcgcgg
      601 tgatgctggc gaaggcgtag tgggcataga agtagatgag cagcaggagg gcgagggccg
      661 cggcccacga ccagccgcgg gtgtaggcgg cggagccttg cgcgaagcgc tcgacgatgc
      721 cggtcttcgc cagctcggac gcgagctgca ccagcccgcc gtaccagatg aacacgtcga
      781 accctgcgcg ctcggcgagc acgtcctccc acacgagtac gcccgtcagc agcagcacgc
      841 agatgccgac gagcgcgacg gtcgcgtagt gcagcccgtg gagcgtgctc gtcatccaca
      901 aggtcgccac gagtgcgaag acgaggagca tcatcttctc gccgagcgac atcggcccca
      961 tgcgccgcag ctcttcggcg gcgagccgcg cggcgtcggg cgtgcgcttg atctcgggcg
     1021 ggttgacgcg atagagcagc ggcgggatga cgagcagcga gacgaggccg ggcacaatcg
     1081 cgccgagcgc ccagcgcgcg taagtcaact cgacgcccgc cgcctctttc gcgaaccgcg
     1141 cgatgatgac gttcgacgcc tgccccgtca ggaacatcgc gcagatgatg acctcgcatt
     1201 ggtagagcaa agggaagagg aaggcgccga gccggcgggc cgtctcgccc ggccttgagt
     1261 cgtaggcttc ggccagagac ttcgcgatgg ggaagatgat gccgcccgag cgcgcgcccg
     1321 tcgaggggat gatcatcgcg agcagcacgt ccgtcaggat cagcgcgtag cccaatccga
     1381 gcgtgcggct gccgatggcg cggatgaaga ggaaggcgat gcggcggccc aaccccgtct
     1441 tgatcatccc gcgcgagatc atgaaggccg cgagcaccat ccagacgacc gggtcggcgt
     1501 agccgccgag cgcgcgctcg atggggagca cgcccagcag cgccgtcgcc gtgacgccca
     1561 acagcacgac cgccgcgccc ggtaggggcc gcacgatcga gccgacgatg gtcgccgcga
     1621 agatcgccag cagccgccac gactgcggcg tcacgcccgc gggcatcggc gcgagcgcga
     1681 tggcgaacgc gcacgccagc acgaccgccc acttgcccgc ggtcgccgcg cggctcgggc
     1741 cgccgtccgt cttcacttct tcctgtgtcg ctctcgcctc gccgggcgtg tcgctcatgt
     1801 gcggaggtca gttaagctat tgcagttcga agggccgcgc ggaggataac acaaaagcgg
     1861 taggcagcag gcggtaggcg gtagacagtc aagacagaaa gagcgcagag gctcggcact
     1921 ttacctccca agcctctccg cccttccttc ttccgacgcc tgacttccga ctactttctt
     1981 ttgctgccaa ccgccaactg cctactgcct gctgcccgct cagcgcgagg tgagtttgag
     2041 gccgacgatg ccggcgacga tgaggaggac gcagagcagt cgcgcggtct cgcgcggctc
     2101 gccgaagagg atgatgccga ggacggctgt gccgacggcg ccgatgcccg tccagacggc
     2161 gtaggccgtg ccgatgggga gcgacttcac cgccaccgcc agcagcagca tgctgaggat
     2221 catggccgcg cccgtccaca ggctcggcca gaggcgcgtg aacccctccg tgtacttcag
     2281 cccgacggcc cacacgactt cgagcagccc cgcgagaaga agatataccc acgccatacg
     2341 attcgacctg cgaccacgat taaaagtact gaggactgag taacagccgt gaaccgtgaa
     2401 ccgtaaaccg tgacgagtcg gaaccggttt tttcttgtca cggttgacgg ttcaccgttc
     2461 acggctattt ttcaccaccc gctggcgaac aacttctgcg cggccttcag cccacacttc
     2521 tggtacgcgt cgtactcttt ggccgcgcgc tcgcggacgc gctcgcccgc ttcgcccgcg
     2581 acccagccat ccaccgccgc cttcaacacg taagcctccg gcgccatcaa attcgtcgtc
     2641 cacagcagcg ggcgcgcgcc cgcctcgcgc agcgcgtcgg cgaagtagcc cttgctcgcg
     2701 cacgccagga tgaccgcgtc gctcgcgccg cccgcgtccg acttcttcgg atagctcgcg
     2761 agcgaaaagt ccatcagccc gttgtgcccg acgtaggcga cgaggtg
//
LOCUS       DBNGOH010000372         4401 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4455197, whole genome shotgun sequence.
ACCESSION   DBNGOH010000372 DBNGOH010000000
VERSION     DBNGOH010000372.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4401)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4401)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4401
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4455197"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1741
                     /locus_tag="AC159P_07375"
     CDS             <1..1741
                     /locus_tag="AC159P_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017915674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR3695312.1"
                     /translation="EIAKTLAEFLFGSPERMIRLDMSEFQTPDALDRVLGESQETTSD
                     SAALVILIRKQPFSVVLLDEFEKAHPNVWDLFLQVFDDGRLTDRRGNTADFRHCVVIM
                     TSNLGATLPHGATVGFSGEQAQFTSAGVERAVGRSFRREFVNRIDRVVVFRPLGMGVM
                     RELLRKELNDVLHRRGLRTRQWAVEWDESAIEFLLQKGFTADLGARPLKRAVERYVLS
                     PLAMTIVNHQFPEGDQFLFVRSAPDGESIDVQFVDPDAPDEASTQEAAAAELPEETEG
                     DADGEVRLESIALDARGTPEEVRLLQSSYRELAERTDADEWHERKASALGETQAPGFW
                     DSPARFAVLGLAEYMDRIEAGLDTAGSLLGRLTGSRDRRQFPPRMVARVAEQLYLVGT
                     ALAGLEAGEPRDAFLSVHASREAGADKATSDRLARALTQMYRNWAEKRRMRFEVLEET
                     DGVGPDDGDDGEGVQRGYRALAAVSGFGAYTILRPEAGLHVMETPQDEKSFNRAKAVV
                     RVVAQPDEPAGHGEGAVRRQAERALDREANAPQTIVRRYREEPSPLVRDSARGWRTGK
                     IERVLGGDFDLLS"
     gene            complement(1983..2384)
                     /locus_tag="AC159P_07380"
     CDS             complement(1983..2384)
                     /locus_tag="AC159P_07380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695313.1"
                     /translation="MWLQKNLHSDDERRILSADSDDAPIGSASNGLLQAVSHYQLQLK
                     TAPLGEHMKNLRKLFAVTLLVLTLGLPAYADGQMETPKPSGGSPVKMAQPLETPLIQD
                     VESDTSGTQSGQMETPMTLMLSVIESVLALI"
     gene            2402..4357
                     /locus_tag="AC159P_07385"
     CDS             2402..4357
                     /locus_tag="AC159P_07385"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3695314.1"
                     /translation="MNLEVKLLDRIDDPALSRAERMVTRCRLARELEEAGDFEGARAA
                     VAEVWQRVGERPVLDGLDRAASAEVLFAVGRLTGSIGSARQIEGAQEAAKDLISESIT
                     LFEQMRAPRRVSEALVDLALCYWRQGAHDEARVTLREVLRRLGDGGGELKAVALLRSA
                     IVEKTAFRHNDALRIYTEAAPLFDAIESHALKGKFHNGFANVLENLSREERRDDYRDR
                     ALVEYAAASFHFEQAGHMRYCARVENNLGFLFFTIGNFADAHEHLDRARGLFAGLKDS
                     GSVAQVDDTRARAFIAEGRHAEAERIARSSVRSLEGGDEQALLAEALRTHGVALLRLG
                     QHEVARRTLLRAAETAEQAGDREGAGQAALSVIEELGERLSAGELRELYERADSLLAG
                     ARNMDALARLNACARLVLQAVAARAEAPRQTAPAAVPAEDFFAPLARGWDGFSLKKEV
                     RRYEAALIERALKESEGVVSRAAQMLGFEHYQTLIALLNNRHKHLLHARTPIVPRRRS
                     IIRLRAPRSTPSHRVERKLRPVTILHVEDNGLVADAVKETLELEGWQVVPCADGLQAL
                     SEVASEEPLDLLLLDNNLPGLNGVEIVRRVRSMAHRRDTPVVIISATECEEEAIGAGA
                     DAFLRKPQDISVMVETISRLLEREPVH"
BASE COUNT          723 a         1510 c         1499 g          669 t
ORIGIN      
        1 cgagatcgcc aagacgctcg ccgaatttct gttcggctcg ccggagcgga tgatccgtct
       61 cgacatgagc gagttccaga cgccggacgc gctcgaccgc gtgctcggcg agtcgcagga
      121 gacgacgagc gacagcgcgg ccctcgtcat cctcatccgc aagcagccct tctcggtcgt
      181 cctgctcgac gagttcgaga aggcgcatcc gaacgtgtgg gacctgttcc tccaggtctt
      241 cgacgacggg cggctcacag accggcgcgg caacacggcg gacttccgcc actgcgtcgt
      301 catcatgact tcgaatctgg gggcgacgct cccgcacggc gccacggtcg gcttctcggg
      361 cgagcaggcg cagttcactt ccgccggagt cgagcgcgcg gtcgggcgct cgttccgccg
      421 cgagttcgtc aaccgcatcg accgcgtcgt cgtcttccgc ccgctcggca tgggcgtgat
      481 gcgcgagctg ctcaggaaag agttgaacga cgtgctgcac cggcgcggcc tacgcacgcg
      541 gcagtgggcc gtcgagtggg acgagtcggc catcgagttt ctgttgcaga aggggttcac
      601 cgcagacctc ggcgcgcggc ccctgaagcg cgccgtcgag cgctacgtgc tctcgccgct
      661 cgcgatgacc atcgtcaacc accagttccc cgaaggcgac cagttcctct tcgtccgctc
      721 cgcgcccgac ggcgagagca tcgacgtgca gttcgtagac ccggacgcgc ccgacgaggc
      781 atcgacgcag gaagcggcgg cggcggagtt gccggaggag acggaaggcg acgcggacgg
      841 cgaggtgcgg cttgagtcca tcgcgctcga cgcgcgcggc acgcccgaag aggtgcggct
      901 gctccagtcc tcctaccgcg aactcgcgga gcggacggac gcggacgagt ggcacgagcg
      961 taaagcgtcc gcgctcggcg agacgcaggc gcccggcttc tgggactcgc ccgcgcgttt
     1021 cgccgtgctc ggactcgccg agtacatgga tcgcatcgag gcaggactcg acaccgcagg
     1081 ctctctgctc ggtcggctca ccggctcgcg cgaccgcagg cagttcccgc cgcgcatggt
     1141 cgcgcgcgtg gccgaacaac tctacctcgt cggcacggcg ctcgccgggc ttgaggcggg
     1201 cgagccgcgc gacgccttcc tctccgtgca cgccagccgc gaggccggcg ccgacaaagc
     1261 gaccagcgac cgcctcgcac gcgctctcac gcagatgtac cgcaactggg ccgagaagcg
     1321 ccgcatgcgc ttcgaggtgt tggaagagac ggacggcgtg gggcctgacg acggcgacga
     1381 cggcgagggc gtgcaacgcg gctaccgcgc gctcgcggcg gtgtccggct tcggcgccta
     1441 cacgattctg cggcccgagg cggggctgca cgtcatggag acgccgcagg acgagaagtc
     1501 cttcaaccgc gcgaaggccg tcgtgcgcgt ggtcgcgcag ccggacgagc ctgcgggcca
     1561 cggcgaaggg gctgtgcgac gacaggccga gcgcgcgctc gaccgagaag cgaacgcgcc
     1621 ccaaacaatc gtccgccgct accgcgaaga gccttccccg ctcgtccgcg actcggcgcg
     1681 cggctggcgc acgggcaaga tcgagcgcgt gctcggcggc gacttcgacc tgctctcctg
     1741 acgcgtggac gggccgccgt cgcccgctgc cggtcgcctc gatacgatac gccgcgggcc
     1801 gccgcacgtt accaagaaat ttgttgcttt ctgtgcggca gcgggttaat ctgcccccat
     1861 tgagtgcctc ctcgccgggc ataccggcat ccggcactca ctcggcggag acaccaatcc
     1921 cgaaccgccc gtgcccgggg ccacggcagt tattcaggct tcgagacttg gagggatttg
     1981 gcttagatca atgccaggac gctttcgatc acgcttagca tcaaggtcat cggggtttcc
     2041 atctggccgc tctgcgtccc cgaagtgtcg gactcaacgt cttgtattaa cggcgtttcc
     2101 agcggctggg ccatcttgac cggtgagccg ccggaaggct tcggggtctc catttgcccg
     2161 tccgcgtagg cgggaagtcc gagcgtcaga accagcagag tgacggcgaa gagtttacgc
     2221 agatttttca tatgttctcc caatggtgca gttttgagtt gtaattggta atgagaaaca
     2281 gcctgaagca gcccattcga ggccgagcct ataggcgcat cgtccgaatc agcggataga
     2341 atgcgccttt cgtcgtctga atgcaaattt ttttgtaacc acatagaaag ggcgccctca
     2401 catgaactta gaggtgaaac tactcgaccg aatcgacgac ccggccctct ctcgcgcgga
     2461 acgcatggtg acgcgctgcc ggctcgccag agaactcgaa gaagcgggcg atttcgaagg
     2521 cgcgcgcgcc gcggtggccg aagtgtggca acgcgtgggc gagcggcccg tgctggacgg
     2581 actcgaccgc gcggcgtcgg cggaagtgct cttcgccgtc ggcaggctca ccgggagcat
     2641 cggcagcgcc cggcagatcg aaggcgcgca ggaggcggcc aaagacctca tcagcgagag
     2701 catcaccctc ttcgagcaga tgcgcgcgcc gcggagggtc tcggaagcgc tggtcgatct
     2761 cgcgctctgc tactggcggc agggcgcgca cgacgaggcg cgcgtgacgc tgcgcgaagt
     2821 gctgcggcgg ctcggcgacg gcgggggcga gctgaaagcg gtcgccctcc tgcgcagcgc
     2881 catcgtcgag aagacggcct tccgccataa cgacgcgctc cgcatttaca cggaggcggc
     2941 tccgctcttc gacgcgatcg agagccacgc gctgaagggc aaattccata acggcttcgc
     3001 caacgtcctt gagaacctga gccgcgagga gcggcgtgac gactacaggg atcgcgcgct
     3061 cgtcgagtac gccgcggcca gcttccactt cgagcaggcc gggcacatgc gctactgcgc
     3121 gcgcgtcgag aataacctcg gcttcctctt tttcaccatc ggcaatttcg ccgatgcgca
     3181 cgagcacctc gaccgcgcgc gcgggctgtt cgcgggcctg aaggacagcg gcagcgtggc
     3241 gcaggtggac gacacgcgcg cgcgcgcctt catcgccgaa gggcggcacg cggaggccga
     3301 gcggatcgcg cgctcatccg tgcgctcgct cgaaggcggc gacgagcagg cgctcctcgc
     3361 cgaggcgctg cggacgcacg gcgtggcact cttgcggctc ggccagcacg aggtcgcgcg
     3421 ccggacgctg ctgcgcgccg cggagaccgc cgagcaggcg ggcgaccgcg agggcgccgg
     3481 gcaggccgcc ctctccgtca tcgaagagct cggcgagcgc ctttcggcgg gcgagttgcg
     3541 cgagctttac gagcgcgccg actcgctgct cgcgggcgcg cgcaacatgg acgcgctcgc
     3601 gcgcttgaac gcctgcgcgc gtctcgtgct gcaggccgtg gccgcacgcg ccgaggcacc
     3661 ccggcagacg gcgccggcgg ctgtccctgc ggaggacttc ttcgcgccgc tcgcgcgcgg
     3721 gtgggacggc ttctctttga agaaggaagt gcgccgctac gaggcggcgc tcatcgagcg
     3781 cgcgctgaag gagtcggagg gcgtcgtctc gcgcgccgcg cagatgctcg gcttcgagca
     3841 ctaccagacg ctgatcgcgc tcctcaacaa ccgccacaag cacctcctgc acgcgcgcac
     3901 gcccatcgtc ccgcgccgcc gcagcatcat ccgcctgcgc gccccgcgca gcaccccttc
     3961 gcaccgcgtc gagcgcaaac tgcgccccgt cacgatcctc cacgtcgagg acaacgggct
     4021 ggtcgccgac gccgtcaagg agacgctcga actcgaaggc tggcaggtcg tgccctgcgc
     4081 cgacggcctt caggcgctct cggaggtcgc cagcgaagag ccgctcgacc tgctgctgct
     4141 cgacaataac ctgcccggcc tgaacggcgt cgaaatcgtg cggcgcgtgc gctcgatggc
     4201 ccaccgccgc gacacgccgg tcgtcatcat ctcggcgacc gagtgcgagg aggaggcgat
     4261 aggcgcgggc gccgacgcct tcctcagaaa gccgcaggat atctccgtca tggtcgagac
     4321 catctcgcgg ctgctcgaac gcgagccggt tcattaaagc cgtcggcgcg ttacttcaat
     4381 cgacgttaag aggcggtctt c
//
LOCUS       DBNGOH010000373         3113 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_239208, whole genome shotgun sequence.
ACCESSION   DBNGOH010000373 DBNGOH010000000
VERSION     DBNGOH010000373.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3113)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3113)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3113
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_239208"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..360
                     /locus_tag="AC159P_07390"
     CDS             <1..360
                     /locus_tag="AC159P_07390"
                     /inference="COORDINATES: protein motif:HMM:NF024591.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding enzyme"
                     /protein_id="HIR3695315.1"
                     /translation="EDIEAALDRQPEIKLSAVVGVEGPRGPEPLAVLILKEPDTDPTP
                     AVERANETLARHQHVRRWAVWPDTDFPRTATQKVRKRDILEKLDGETGRRGDRETVER
                     GDVLAGVSPRLPVSPSS"
     gene            311..1279
                     /locus_tag="AC159P_07395"
     CDS             311..1279
                     /locus_tag="AC159P_07395"
                     /inference="COORDINATES: protein motif:HMM:NF013702.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HIR3695316.1"
                     /translation="MFWLGFLPVSPSPRLLDQSLIPSLAGLDSSAKLATDLKLDSLGR
                     VELLSAIEDRYQIEIDESAFTAATTLGDIERLVRKGGEGGDTSSRYPYPRWAQRFPVT
                     WFRVAFYYAVVLPITSVLCWVRVRGAEHVRDLRGPVLFVSNHVTFVDHALIMSALPGR
                     FRRGLAIAQEGERLRWWRRPPAGTPLLKRVKWLAQYALVVSVFNTFSLPQKSGFRRSF
                     AFAGESVDRGYSLLVFPEGTRTEDGRPNPFMRGTGLLASQLRIPVVPVRIDGLFELKQ
                     QGRRFLSPPGAVSVTFGAAVNYPPGEEPARITADLERRVMNLGGKV"
     gene            complement(1322..1969)
                     /locus_tag="AC159P_07400"
     CDS             complement(1322..1969)
                     /locus_tag="AC159P_07400"
                     /inference="COORDINATES: protein motif:HMM:NF017496.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HIR3695317.1"
                     /translation="MNKTDDGVWPRRSGEMTMSLPRALPTFTPEEYLSLERVSEIRHE
                     YLDGFVYAMAGESPTHSIICFNLAGSLHFMLKGTPCRGYSPNMKVRTKAESLYSYPDL
                     VVVCGEPVYHDKHGDVLVNPTVVFEVLSPSTEAYDRGEKSLRYRTEIESLRDFVLVAQ
                     DRPRAEHFSRRPDGAWSRNEVSGLDGVLPLASIDCRIRLADVYDRIAYAEHPAQR"
     gene            2227..>3113
                     /locus_tag="AC159P_07405"
     CDS             2227..>3113
                     /locus_tag="AC159P_07405"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: protein motif:HMM:NF003652.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HIR3695318.1"
                     /translation="MLYRSILRPLLFRLPPETAHEMALSALAIALKPEPLRRLAAWRF
                     GRAAPGALTRFGLRFPNPVGLAAGFDKNGKVYEGMCALGFGFVEVGTVTHQPQPGNPH
                     PRLFRLPLDRALVNRQGFNNEGAAALARRLERRAGGACVLGVNIGKSRVVDIEDATAD
                     YLASFRLVQHHADYVTVNVSSPNTPGLRELQRADRLFALLRELQNSNRQLADETARVP
                     VPLLVKVAPDLDEAGLEMIVDVARRTGVAGLIATNTTTSRDGLRTPADVVASCGEGGL
                     SGAPLRARSTRMIAALRRLT"
BASE COUNT          492 a         1088 c         1011 g          522 t
ORIGIN      
        1 gaagacatcg aggcggcgct cgaccgtcaa cccgaaatca agctcagcgc agtcgtcggc
       61 gtcgaaggcc cgcgcggccc cgagccgctc gccgtgctca tcctcaagga gccggacaca
      121 gaccccacgc ccgccgtcga acgcgccaac gagactctcg cgcggcacca gcacgtgcgc
      181 cgctgggccg tctggcccga cacggacttc ccgcgcaccg cgacgcagaa agtgcgcaag
      241 cgcgacatcc tcgaaaagct ggacggggag acggggagac ggggagacag ggagaccgtg
      301 gaacgaggag atgttctggc tggggtttct ccccgtctcc ccgtctcccc gtcttcttga
      361 tcaatccctc atcccttcgc tggcgggtct cgactcgtcg gcgaaactgg cgacggactt
      421 gaagctggac tcgctcggtc gcgtcgagtt gttgagcgcg atcgaggatc gctaccagat
      481 agagatcgac gagtctgcct tcaccgccgc caccacgctc ggcgacatcg aacggctcgt
      541 gcgcaaaggg ggcgagggcg gcgacacttc gtcgcgctac ccttacccgc gttgggcgca
      601 gaggttcccc gtgacatggt ttcgggtcgc gttctattac gcggtcgtgc tgccgatcac
      661 ttcggtgctg tgctgggtgc gcgtgcgggg cgcggagcac gtgcgggact tgcgcgggcc
      721 ggtgctgttc gtctcgaacc acgtcacgtt cgtcgatcac gcgctcatca tgtcggcgct
      781 gcccggcagg ttccggcggg gactggcgat tgcgcaggag ggcgagcgcc tgcgctggtg
      841 gcgccgcccg cccgccggta cgccgctgct gaagcgcgtg aagtggctcg cgcagtacgc
      901 gctcgtcgtc tccgtcttca acacgttctc tctgccgcag aagagcggct ttcgccgcag
      961 cttcgccttc gcgggcgagt cggttgaccg cggctacagc ctcctcgttt tccccgaagg
     1021 cacgcgcacc gaagacgggc gccccaaccc cttcatgcgc ggcacggggc tgctcgcctc
     1081 gcagctccgc atccccgttg tccccgtccg catcgacgga ctgttcgagc tgaagcagca
     1141 gggccggcgc ttcctctccc ctcccggcgc ggtctccgtc accttcggcg cagccgtcaa
     1201 ctacccgccc ggcgaagaac ccgcgcggat caccgccgac cttgagcggc gcgtgatgaa
     1261 tttgggaggt aaagtgtgaa ggcgtctcgc cgcaggatga tgggtcgcgc gccgagcctg
     1321 gtcaacgttg cgcgggatgt tcggcgtagg cgatgcggtc gtacacgtct gcgagccgga
     1381 ttctgcaatc aatcgaagcg agcggcagga ctccgtccag accgctgact tcgttgcgcg
     1441 accacgcgcc gtcgggccgg cgcgagaagt gctcggcgcg gggccggtct tgcgcgacca
     1501 gcacgaagtc ccgcaacgac tcgatctcgg tgcggtagcg cagagacttt tcgccgcggt
     1561 cgtaggcttc ggtcgaaggc gacaagactt cgaagacgac cgtcgggttg acgagcacgt
     1621 cgccgtgctt gtcgtggtac acaggctcgc cgcagaccac caccagatcg gggtacgagt
     1681 agagactctc cgccttcgtc ctcaccttca tgttcggcga gtaaccgcgg caaggcgtac
     1741 ctttgagcat gaagtgcaag cttcccgcca agttgaaaca gatgatgctg tgggtcgggc
     1801 tttcacccgc catcgcgtag acgaagccgt ccaggtactc gtgccgaatc tcggagacgc
     1861 gttcgagcga caaatattct tccggggtga acgtcggcag cgcgcgcggt agggacatcg
     1921 tcatctcccc cgaccggcgc ggccagacgc cgtcatctgt tttgttcatc agacctcttc
     1981 ttcgacacaa ctgcgaccgt cgctgcggat tctacgcccg gccccgcacc ttcacaacca
     2041 caatcggatt tgcaacagac attgcaacgc cccgaggaga gcttctttga caggatttcc
     2101 gccgctctgc tacgctcaag gtcgctttag cacgcactcc cctgttggtg ttgttcgaag
     2161 aggcgcgggc ggtctcaaag gggactggcc ggcgcgctcg cgcgcttcgg acgactgacg
     2221 cggttcatgc tctatcgctc tatcctgcgc cccctgttgt tccgactgcc cccggagacg
     2281 gcccacgaga tggcactgag cgcgctcgcg attgccctga agccggagcc gctgcgtcgg
     2341 ctcgccgcgt ggcgattcgg gcgcgccgcg ccgggcgcgc tcacgcgctt cggcctgcgc
     2401 ttcccgaacc cggtcggcct cgccgccggc ttcgacaaga acgggaaggt ctacgaaggg
     2461 atgtgcgcgc tcggcttcgg cttcgtcgag gtcgggacgg tgacgcatca gccgcagccc
     2521 ggcaacccgc acccgcgcct cttccgcctg ccgctcgacc gcgcgctcgt caaccgccag
     2581 ggcttcaaca acgaaggcgc ggcggcgctc gcgcgcaggc tcgaacggcg cgcgggcggg
     2641 gcttgcgtgc tcggcgtcaa catcggcaag agccgcgtcg tggacatcga ggacgcgacc
     2701 gccgactacc tcgccagctt ccgcctcgtc cagcaccatg ccgactacgt caccgtcaac
     2761 gtcagctcgc cgaacacgcc cggcctgcgc gagctgcaac gcgccgaccg cctcttcgcg
     2821 ctcctccgcg agctgcaaaa cagcaaccgg caactcgcgg acgagacggc gcgcgtgccc
     2881 gtccctttac tcgtcaaggt cgcgcccgac ctcgacgaag cagggcttga gatgatcgtt
     2941 gacgtggcgc ggcgcacggg ggtggcaggg ctgatcgcga ccaacacgac gacgagccgc
     3001 gacggccttc gcacccccgc cgacgtggtc gcctcgtgcg gcgagggcgg actcagcggc
     3061 gcccccttgc gcgcgcgctc gacgcggatg atcgccgcgc tgcggcgcct gac
//
LOCUS       DBNGOH010000374         3821 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1340035, whole genome shotgun sequence.
ACCESSION   DBNGOH010000374 DBNGOH010000000
VERSION     DBNGOH010000374.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3821)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3821)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3821
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1340035"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            196..1368
                     /locus_tag="AC159P_07410"
     CDS             196..1368
                     /locus_tag="AC159P_07410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695319.1"
                     /translation="MNHLRTSSKCFRSCRAILPALILAACAASVRGQARPPVAVEHVA
                     FKNFPVEMVGVELAGELHNLAEKKPALFGVSFDAPEDWLRHVSFRLRNKTDKTLLSVT
                     LSGWLAVGEAGEVPMGIDLLYGQESDESAFTGRAPRGEPLRLAPGETADARWSAAEYT
                     ELDRFLSMKRPVAGYRRMRLDLREARFEDGTVWSMGKLYRINPHDPRKWTPLDKDETG
                     RAAPPDLKPGERVVEVPSYKPDDDPKTLAISEIKVAGQPVTPGRPFFAGEDWLRRLTV
                     RVKNTSAKPILSVQVSFGLPEATYRAGGVGLRLRYGRETATGGRAVPDAKPLLPGEEA
                     ELSFTDGEYETQRSFAERRSGAVSFTRVRLGGASLVFVDGTRSFVSNPAHALKEEK"
     gene            1479..1955
                     /locus_tag="AC159P_07415"
     CDS             1479..1955
                     /locus_tag="AC159P_07415"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016867505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3695320.1"
                     /translation="MEWHRGRYTISTDRARLDIKAVHAFLSTSSYWAQGRSFETVARA
                     VENSLPFGLYDGERLLGFARVVTDHATFAWLADVYVLEEFRGEGLGKFLVETVLDHPD
                     FRTLRRWLLGTRDAHGLYRRFGFTQIEEPQFYMHRVNTNERGQPSATTTAATDEGG"
     gene            2169..3041
                     /locus_tag="AC159P_07420"
     CDS             2169..3041
                     /locus_tag="AC159P_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013580980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     polyprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA-like polyprenyltransferase"
                     /protein_id="HIR3695321.1"
                     /translation="MVTAVRNIRTTLEMIKIEHTLFALPFAFLGAVLAAGGLPTARQA
                     LWITLAMVGARSAAMAFNRIADRAYDAANPRTAARAIPAGQLSLPFAWAFTAASAALF
                     FLAAAMLNRLTLLLAPVALASVLAYSYTKRFTSLSHVVLGWCLSIAPTGAWVAVRGAL
                     DSPVPLLLSLVVLLWTAGFDVLYACQDYDFDRRAGLRSIPARVGVARALWVSRLLHAG
                     AFAALVGLYFLTGLGALALAGVLATGALLVYQHSLVRADDLSRLNAAFFTTNAFVSVI
                     LLATFGGALFLTGL"
     gene            3046..>3821
                     /locus_tag="AC159P_07425"
     CDS             3046..>3821
                     /locus_tag="AC159P_07425"
                     /inference="COORDINATES: protein motif:HMM:NF015983.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HIR3695322.1"
                     /translation="MNFSHDPKLLELAAKVEAGERLTFADGLALFATADVPALGRLAD
                     SVRRRLHGRTTYYNVNRHFNPTNICYADCKFCGFFRTPRAADAYTHNIEDSLRIAGEA
                     VREGATELHIVGGLNTKLPFNYFTDLLGSLKREYPRLHLKAWTMVELDHFAHFYKMTD
                     EDVIEQLKRAGMDSCPGGGAEIFAEPTRSKICAHKTNAERWLELAEKVHAAGLKTNAT
                     MLYGHIESLEDRVDHLVRLREQQDKSGGFQCFIPLAFYPPG"
BASE COUNT          602 a         1306 c         1268 g          645 t
ORIGIN      
        1 caccgcccgc agacgatcga agagctggtg gatcacctcg tcttccgcat cctcgaccag
       61 ttcggcatcc cgcacccgac gggcacgcgc tggaagggca cggaggcgac cgacgacctg
      121 cgccccgtcc acgccgccga atgaccgact cgcacggcgg ccttcgccgg ccccgcgcgc
      181 cgcacgagag gagagatgaa tcacttgaga acctcttcga agtgtttccg ttcatgccga
      241 gcgatccttc cggcgctgat tcttgccgcg tgcgcggcgt ccgtccgtgg gcaggcgagg
      301 ccgcccgtcg ccgtcgagca cgtcgcgttc aagaatttcc cggtcgagat ggtgggggtg
      361 gagctggccg gcgagcttca caacctcgcc gagaagaagc cggcgctgtt cggcgtctcc
      421 ttcgacgcgc ccgaagactg gctgcggcac gtctcgttca ggctcagaaa caagaccgac
      481 aagacgctgc tcagcgtgac gttgagcggt tggctggcgg tcggagaggc gggcgaggtg
      541 ccgatgggta tcgacctgct ttacggtcag gagtcggacg agtcggcgtt cacgggccgc
      601 gcgccgcgcg gcgagccgct ccggctcgcg cccggcgaga cggcggacgc tcgctggtcg
      661 gcggcggagt acacggagct ggacaggttc ctttcgatga agcgcccggt cgccggctat
      721 cgacggatgc gcctcgacct gcgcgaggcc cgcttcgagg acgggacggt gtggagcatg
      781 gggaagctct accgcatcaa cccgcacgac ccgcgcaagt ggacgccgct cgacaaggat
      841 gagacgggcc gcgcggcgcc gcccgacctg aagccgggcg agagggtggt ggaagtcccc
      901 tcttacaagc cggatgacga tccgaagacg ctggcgatca gcgagatcaa ggtggcgggg
      961 cagcccgtca cgccggggcg tccgttcttt gcgggcgaag actggctgcg gcgtctgacg
     1021 gtgcgcgtca agaacacctc cgccaagccg atcctctccg tgcaggtgag cttcggcctc
     1081 cccgaagcca cctatcgcgc gggcggcgtg ggcttgcgcc tccgctacgg cagggagacc
     1141 gcgaccggcg gacgcgccgt gcccgacgcg aagccgctgc tgcccggcga ggaggcggaa
     1201 ctgagtttca ccgacggtga gtacgagacg cagaggagct tcgcggagcg gcggagcggg
     1261 gccgtcagtt tcacgagagt acgtctgggc ggggcgtctt tggtcttcgt tgacggcaca
     1321 agatcgttcg tctccaaccc ggcgcacgcg ctgaaggagg agaaatgatg agtgcggaat
     1381 gatgaatgat gaatgaggag cacgccgact ctcgccgttc ggcgtccttc tcgctcaacc
     1441 ggtctttcat tccgcattcc gcattcatcg ttatctttat ggagtggcac aggggccggt
     1501 acacgatcag caccgaccgg gcgaggctcg acatcaaggc cgtccacgcg tttttgagca
     1561 cgtcgagcta ctgggcgcag gggcgctcgt tcgagaccgt cgcccgcgcg gtcgagaact
     1621 ctttgccgtt cggactctac gacggcgagc ggttgctcgg cttcgcgcgc gtcgtgaccg
     1681 accacgcgac cttcgcgtgg ctggccgacg tgtacgtgct cgaagagttc cgcggcgagg
     1741 gcctcggcaa gttcctcgtc gagaccgtgc tcgatcatcc cgacttccgc accctgcggc
     1801 gctggctcct cggcacgcgc gacgcgcacg gcctctaccg ccgcttcggc ttcacgcaga
     1861 tcgaggagcc gcagttctac atgcaccgcg tcaacacgaa cgagcggggc caaccttccg
     1921 caacgaccac ggcggccacg gacgaaggcg gctgaccgcc gttggcactg tgccacgacg
     1981 gggccgaggc gtggcgattt gcaaagcgcg ggcgccgcgc gcggacgttt tcacaccgct
     2041 ttccgagcgg cgcgcgcttt gccttatgta aggacggaga agaaggatga cgattgacga
     2101 gagcggcgct ctcgtcaatc gtcactgatt gaattcatca ttcatcattc agcgttccgc
     2161 attcatctat ggtgacagcc gtccgcaaca tccgcacgac gctcgaaatg atcaagatcg
     2221 agcacacgct gttcgcgctg cccttcgcgt ttttgggggc ggtgctggcc gccgggggtc
     2281 ttccgaccgc gcggcaggcg ctctggatca cgctggcgat ggtcggcgca cgctccgccg
     2341 cgatggcctt caaccgcatc gccgaccgcg cctacgacgc cgccaacccg cgcaccgccg
     2401 cgcgcgccat ccccgccggg cagctctcgc tccccttcgc gtgggccttc accgcggcct
     2461 ccgccgcgct cttcttcctc gcggcggcga tgctcaaccg gctgacgctc ctgctcgccc
     2521 cggtcgcgct cgcgagcgtc ctcgcctact cgtacacgaa gcgtttcacc tcgctctcgc
     2581 acgtcgtcct cgggtggtgt ctctcgatcg cgccgacggg cgcgtgggtc gccgtgcgcg
     2641 gcgcgctcga cagccccgtg ccgctcttgc tctcgctcgt cgtgctgctg tggactgccg
     2701 gcttcgacgt gctctacgcc tgccaggatt acgacttcga ccggcgcgcg gggctgcgct
     2761 cgatccctgc gcgggtgggc gtcgcgcgcg cgctgtgggt ctcgcggctc ctgcacgcgg
     2821 gggcgttcgc ggcgctcgtt gggttatact ttctgaccgg cctgggcgcg ctcgcgctcg
     2881 cgggcgtgct ggcgaccggc gccctgctcg tctaccagca ctcgctcgtg cgcgccgacg
     2941 atctgtcgcg gctcaacgcg gcgttcttca cgacgaacgc cttcgtcagc gtcatcctgc
     3001 tggcgacgtt cggcggcgca ttgttcctga ccggacttta aagcgatgaa cttttcacac
     3061 gacccgaaac tcttggaact cgccgcgaag gtcgaagcgg gcgagcggct cacgttcgcg
     3121 gacggcctcg cgctgttcgc caccgcggac gtgcccgcgc tcggtcggct ggccgactcc
     3181 gtgcggcggc ggctgcacgg gcggacgacg tactacaacg tcaaccgcca cttcaacccg
     3241 accaacatct gctacgccga ctgcaagttt tgcggcttct tccgcacgcc gcgcgcggcg
     3301 gacgcctaca cgcacaacat cgaagacagc cttcgcatcg cgggcgaggc cgtgcgtgag
     3361 ggcgcgaccg agctgcacat cgtcggcggg ctgaacacga agctgccctt caactacttc
     3421 acggaccttc tcgggtcgct caagcgcgag tacccgcgcc tccacctgaa ggcgtggacg
     3481 atggtcgagc tagaccactt cgcgcacttc tacaagatga cggacgagga cgtgatcgag
     3541 cagttgaagc gtgccgggat ggactcgtgc cccggcggcg gggcggagat tttcgccgag
     3601 ccgacgcgct cgaaaatctg cgcgcacaag acgaacgccg agcgctggct ggagctggcc
     3661 gagaaggtac acgcggcggg actcaagacc aacgcgacga tgctctacgg ccacatcgaa
     3721 tcactcgaag accgcgtcga tcacctcgtc cgcctgcgcg aacagcagga caaatcgggc
     3781 ggcttccagt gcttcatccc gctcgccttc tacccgccgg g
//
LOCUS       DBNGOH010000375         6367 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4089017, whole genome shotgun sequence.
ACCESSION   DBNGOH010000375 DBNGOH010000000
VERSION     DBNGOH010000375.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6367)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6367)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6367
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4089017"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..889
                     /locus_tag="AC159P_07430"
     CDS             <1..889
                     /locus_tag="AC159P_07430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695323.1"
                     /translation="KKDAKGAEADKPKEPPPQGTPVLWRERNIESLDLFRGEGQQPDL
                     SKITFVREDTSGTSKKYRVTDAAGREWVVKVGSEAQSETAAVRLVWAAGYQTEIVHLA
                     PSVDIVGKGNFKNVRFELRTKERKRHGHWLWAENPFAGKRELQGLKLLMALINNGDLK
                     DDNNVIISGAAPGEVHYAISDLGATFGKTGSGALWKLKRSRNNPEDYAKSKFINGVKD
                     GRVDLTFSGVNAEMMDNITVADARWLGQLLARLSDKQLGDAFRAANYTPAEITMLTQA
                     ARARINELARLPEGTVSRQ"
     gene            complement(1066..2418)
                     /locus_tag="AC159P_07435"
     CDS             complement(1066..2418)
                     /locus_tag="AC159P_07435"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015217379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent decarboxylase
                     family protein"
                     /protein_id="HIR3695324.1"
                     /translation="MLEKDSLRALRAALDRLEAGFDGLPPLDTQLAGAGEFEAVLSEV
                     AERLRDNYPYFHPLYAGQMLMPPHPVARAAYALAMWLNPNNHALDGGRASSAMEKEAV
                     AELARMCGWDDGEHLGHLTGGGTMANLEALWVAGQLRPGALVVASEQAHYTHRRIGGV
                     LGLRFESVACDRRGRMNVGELERLLGREEVGCVVATMGTTAVGSVDPLGELLRLRERH
                     GFRLHADAAYGGYFRLADNLGAEARAAFACLGEVDSIVIDPHKHGLQPYGCGCVLFRD
                     PAVGALYKHDSPYTYFSSAELHLGEISLECSRPGAAAVALWATQRLLPLVAGGEFARM
                     KSDSRAAAVGLYERLRADERFVTAFEPELDIVVWAARAATASEASARARSSFDEAARR
                     GLHLALAELPFDFFEADARGMQRDRDTLTCLRSVLMKPEHLHWVERIWRILDAAGSRQ
                     "
     gene            2519..3427
                     /locus_tag="AC159P_07440"
     CDS             2519..3427
                     /locus_tag="AC159P_07440"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HIR3695325.1"
                     /translation="MRGDLSKHHEPLTGLFPRPASAQEWGRYRLTREQVEFYREHGYL
                     AGVRVLDEEQVERLRAELSELMEPRQPLRHLFYEYHSNESADPERVLFHALGAWRVAP
                     AFHDLLWNPRFLMPAAQLLGGAVRFWHDQLFSKPARHGGVVAWHQDYSYWTRTTPLAH
                     LTCWTGLDDSTRENGCVHYVPGSHLWPDLPVTGLAGDMDAIRTVLTGEQRKLFRPVAV
                     ELKAGEASFHHPRTIHGSYANRAARPRRAAVVNVFRDGVRSNSDEPLLEGVPVIPKGE
                     PMGGRFFPLLFDPSTVRLPGKALRRA"
     gene            3616..3891
                     /locus_tag="AC159P_07445"
     CDS             3616..3891
                     /locus_tag="AC159P_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005988703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HIR3695326.1"
                     /translation="MIDWRVFLTTFGAIFLAELGDKTQLAAMTMAADTKKPLAVFLGS
                     ALALACVSGLAVIVGGTLGHYLPLLWIKRAAAVMFIFIGVMILLGKF"
     gene            4099..>6367
                     /gene="ppdK"
                     /locus_tag="AC159P_07450"
     CDS             4099..>6367
                     /gene="ppdK"
                     /locus_tag="AC159P_07450"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HIR3695327.1"
                     /translation="MSRTSEHKWVYLFDEGSGDARALLGGKGAGLCEMTRAGLPVPPG
                     LVVTTEACNAFYESGKNFPEGMWEQTVEGLRKVEEKTGKRFGDPQNPLLVSVRSGAAF
                     SMPGMMDTVLNLGLNEQTVQGLAAQTGDLRFALDAYRRFASLFGEIVMGVAHEKFERV
                     MGRFKAQTEGGRDTDLSPEQLREVIAAEKQIIFAEQAGTAIPEDPYEQLKVAIAAVFN
                     SWMGRRAVDYRRVNRIPDNLGTAVNVQAMVFGNMGAASGTGVAFTRNPSTGAKELYGE
                     YLLNAQGEDVVAGIRTPNPISQLKAELPEVFDQFKGIAELLERHYRDMQDVEFTIERG
                     RLWMLQTRTGKRTGAAAVRVAVDMVREQVIDKATALRRVTPEQLDQLLHPTVDPKASA
                     TVLATGLPASPGAAQGQVVFDPDEAEQLARDGSKVVLVRQETSPDDFHGMVGAQAIVT
                     ARGGMTSHAAVVARGMGKSCVCGASALDIDYGQQQMSVNGSVVTKGEWITVDGSTGRV
                     FLGQVPTVQPKLGADFRELMKWADEFRVLRVRANADTPHDAAVARDFGAEGIGLCRTE
                     HMFFGDERLAAMREMILADGVGAREKALEKLLPLQRKDFVGIFRAMEGLPVTIRLLDP
                     PLHEFLPNLQDLLGELAELKLKLQRAGSIQEMDRLLDDLDGKRRLLHQVERTRENNPM
                     LGHRGCRLGLVYPEVTRMQARAIFEAACQVQGEGTDIRPEIMVPLVSTAEELRHQEGL
                     IREVARQVMGEHGMTI"
BASE COUNT         1074 a         2170 c         2137 g          986 t
ORIGIN      
        1 gaagaaggac gcgaagggcg cggaggccga caagccgaag gagccgccgc cgcagggcac
       61 gcccgtcctg tggcgcgagc ggaacatcga atcgctcgac ctcttccgcg gcgagggcca
      121 gcagcccgac ctctcgaaga tcaccttcgt tcgggaggac acgagcggca cgtcgaagaa
      181 gtaccgcgtg acggacgccg cgggccgcga gtgggtggtg aaggtcggca gcgaggcgca
      241 gtcggagacc gccgccgtcc ggctcgtctg ggccgccggt taccagaccg agatcgtcca
      301 cctcgcgccg agcgtggaca tcgtcggcaa agggaacttc aagaacgtgc ggttcgagct
      361 gcgcacgaag gagcgcaagc ggcacggcca ctggctctgg gccgagaacc ccttcgccgg
      421 caagcgcgag ctgcaagggc tgaagctctt gatggcgctc atcaacaacg gggacttgaa
      481 ggacgacaac aacgtcatca tctcgggcgc cgcgccgggt gaggtgcact acgccatcag
      541 cgacctcggc gccaccttcg gcaagaccgg cagcggcgcg ctctggaagc tcaagcgcag
      601 ccgcaacaac cccgaagact acgccaagtc gaagttcatc aacggcgtca aggacggtcg
      661 cgtcgatctc accttcagcg gcgtcaacgc cgagatgatg gacaacatca cggtcgccga
      721 cgcccgctgg ctcggccaac tgctcgcgcg cctcagcgac aagcagctcg gcgacgcctt
      781 ccgcgccgcc aactacacgc ccgccgaaat caccatgctc acgcaggccg cccgcgcccg
      841 catcaacgaa ctcgcgcgcc tccccgaagg cacggtcagc aggcagtagg cagcgggcag
      901 caggcagtag gcagttaaaa acagaagaag caagaggctt gagacgggat gtctcaagcc
      961 tcttcacttt actgccagtc gcacgcagag gcttggcact ttacctccca agcctctgcg
     1021 tctttccgac ttccgacttc tgtattctgt ttaactgctt accgcctact gcctactgcc
     1081 tgctgcgtcg agtattcgcc agatgcgttc gacccagtga aggtgctcgg gcttcatcaa
     1141 gacggagcgc agacaggtga gcgtgtcgcg gtcgcgttgc attccgcggg cgtcggcctc
     1201 gaagaagtcg aagggcagct cggcgagcgc gaggtggagg ccgcggcgcg cggcctcgtc
     1261 gaagctggag cgcgcgcgcg cggaggcttc gctcgcggtc gcggcgcgcg cggcccagac
     1321 cacgatgtcc agctccggct cgaacgcggt gacgaatctc tcgtcggcgc gcagccgttc
     1381 gtaaaggccg accgccgccg cgcgcgagtc gctcttcatc cgcgcgaact cgccgcccgc
     1441 gacgaggggc aggaggcgtt gcgtcgccca cagcgccacc gccgccgcgc cgggccgcga
     1501 gcattcgagg ctgatctcgc cgaggtgcag ctccgccgag ctgaagtagg tgtagggcga
     1561 gtcgtgtttg tagagcgcgc ccaccgcggg gtcgcggaag agcacgcagc cgcagccgta
     1621 gggctggagg ccgtgcttgt gcgggtcgat gacgatggaa tcgacttcgc cgaggcacgc
     1681 gaaggccgcg cgcgcctcgg cgccgaggtt gtccgcgaga cggaagtagc cgccgtatgc
     1741 ggcgtcggcg tgcaggcgga agccgtggcg ctcgcgcaga cgcagcagct ccccgagcgg
     1801 atcgacggag ccgacggcgg tcgtccccat cgtcgccacg acgcagccga cctcctcgcg
     1861 cccgagcagt ctctccagct ccccgacgtt catccgcccg cggcggtcgc aggcgaccga
     1921 ttcgaagcgc aggccgagca cgccgccgat gcgtcggtgc gtgtagtgcg cctgttcgga
     1981 ggcgacgacg agcgcgccgg gccggagttg ccccgccacc cacagcgctt cgaggttcgc
     2041 catcgtgccg ccgcccgtca ggtgcccgag gtgctcgccg tcgtcccacc cgcacatgcg
     2101 cgccagctcc gccaccgcct ctttctccat cgcagagctg gcccgcccgc cgtcgagcgc
     2161 gtggttgttc gggttgagcc acatcgccag cgcgtaagcc gcgcgcgcca cggggtgcgg
     2221 cggcatcaac atctgccccg cgtagagcgg gtggaagtat ggatagttgt cccgcagccg
     2281 ctccgcgact tccgacagca ccgcttcgaa ctcgcccgcg cccgcaagct gcgtgtcgag
     2341 cggcggcagt ccgtcgaacc ccgcttcgag ccggtcgagc gcggcgcgca aggcgcggag
     2401 tgaatccttc tctaacaagt gtgaagcggc tccttttaag tgtcgatggt tgagaacgcg
     2461 tacggataat acgccgattc gggttattat caacgtccac ccgctctgcc tgaagcgtat
     2521 gcgcggcgac ctttcaaagc atcacgagcc tctgaccggc cttttcccgc gccccgcgtc
     2581 ggcgcaggag tgggggcggt atcggctgac gcgcgagcag gtcgagttct accgcgagca
     2641 cggttatctg gcgggcgtgc gcgtgcttga cgaagagcag gtcgaacgtc tgcgcgccga
     2701 gctgagcgag ttgatggagc cgcgccaacc gctccggcat ctcttctacg aataccactc
     2761 gaacgaatcc gcagacccgg agcgcgtgct cttccacgcg ctcggggcgt ggcgcgtcgc
     2821 gcccgcgttc cacgacctgc tgtggaaccc gcgcttcctg atgcctgcgg cgcaactgct
     2881 cggcggcgcg gtgcgcttct ggcatgacca gttgttcagc aagcccgcgc gccacggcgg
     2941 cgtggtcgcg tggcatcagg actattccta ctggacgcgg acgacgccgc tcgcgcacct
     3001 cacgtgctgg acgggcctcg acgactcgac gcgcgagaac ggctgcgtcc attacgtgcc
     3061 gggcagtcac ctctggcccg acctccccgt cacgggactc gcgggcgaca tggacgcgat
     3121 tcggaccgta ctgaccggcg agcagcgaaa gctgttccgg cccgtcgccg tcgagctgaa
     3181 ggcgggcgag gcttcgttcc accacccgcg cacgatccac ggctcttacg cgaaccgcgc
     3241 cgcccgcccg cgccgcgcgg cggtcgtcaa cgtcttccgc gacggcgtgc gctcgaactc
     3301 cgacgagccg ctcctcgaag gcgtgcccgt catcccgaag ggcgaaccga tgggaggccg
     3361 gttcttcccg ctcctcttcg acccttcgac agtaagactc cccggcaagg ccctccggcg
     3421 tgcgtgatga ctgtaggggc agggcttgtc cctgcccgct tggactcggt cgattcgatc
     3481 ttgaacggct acgcggctgc gggcagggac tgcccctaca gttctccgcc gcgccctcga
     3541 tctgccgcga cgcattcgcc gtggggggcg cgtggcgcat aatgcgtgta atcttataag
     3601 tgcgaggggt gacgaatgat tgactggcgc gtgtttctga cgacgttcgg ggcgattttt
     3661 ctggccgagc ttggcgacaa gacgcagctc gcggcgatga cgatggcggc ggacacgaaa
     3721 aaaccgctcg ccgtctttct cggctcggcg ctcgcgctcg cctgcgtctc cggcctcgcc
     3781 gtcatcgtcg gcggcacgct cggccactac ctgccgctcc tctggatcaa gcgtgccgcc
     3841 gccgtcatgt tcatcttcat cggcgtgatg atcctgctcg gcaagttctg atagggccgt
     3901 gaaccgtgac aagtgaagaa ctgctttcct cttgtcacgg ttcacggttc acggttcacg
     3961 gcttttaagg ttgcgcgcgc gtccggtgtg ggtgcaaact cttgagcacc gcacgcaggg
     4021 tcttcagccg ccgcttgaag gacgaagggc gcggcggcct ccatctttcc cataacttaa
     4081 gaccggaagg aacctcctat gtctcggacg agcgaacaca agtgggtcta cctcttcgac
     4141 gaggggagcg gcgacgcgcg cgcgctgctc ggcggcaagg gcgcggggct gtgcgagatg
     4201 acgcgggcgg gcttgcccgt cccgcccggc ctcgtcgtca ccaccgaggc gtgcaacgcc
     4261 ttctacgaga gcggcaagaa cttccccgaa gggatgtggg agcagacggt cgaggggctg
     4321 cgcaaggtcg aagagaagac cggcaagcgc ttcggcgacc cgcagaaccc tctgctcgtc
     4381 tcggtgcgct cgggtgccgc gttctcgatg ccggggatga tggacacggt gctgaacctc
     4441 ggtcttaacg agcagaccgt gcaggggctg gccgcgcaga ccggcgacct gcggttcgcg
     4501 ctcgacgcct accggcgctt cgcctctctg ttcggcgaga tcgtgatggg ggtggcgcac
     4561 gagaagttcg agcgcgtgat ggggcgcttc aaggcgcaga ccgagggcgg gcgcgacacg
     4621 gatctgtcgc ccgaacaact gcgcgaagtc atcgccgccg aaaaacagat catcttcgcc
     4681 gagcaggccg gcaccgccat ccccgaagac ccctacgagc aactgaaggt cgccatcgcc
     4741 gccgtcttca actcgtggat gggccgccgc gccgtcgatt accgccgcgt caaccgcatc
     4801 cccgacaacc tcggcaccgc cgtcaacgtg caggcgatgg tcttcggcaa catgggcgcc
     4861 gcctccggca ccggcgtcgc cttcacgcgc aacccctcga ccggcgcgaa ggaactctac
     4921 ggcgaatacc tgcttaacgc gcagggcgaa gacgtggtcg ccggcatccg cacaccgaac
     4981 ccgatcagtc agctcaaggc cgagctgccc gaagtcttcg accagttcaa aggcatcgcc
     5041 gagctgttgg agcgccacta ccgcgacatg caggatgtgg agttcaccat cgagcgcggg
     5101 cggctctgga tgctccagac gcgcaccggc aaacgaacgg gcgccgccgc ggtgcgcgtc
     5161 gccgtggata tggtgcgcga acaggtgatc gacaaggcga ccgcgctccg ccgcgtcacg
     5221 cccgaacaac tcgaccaact cctccacccg accgtcgatc cgaaggcttc ggccaccgtc
     5281 ctcgccacgg gtctgcccgc cagccccggc gccgcgcaag ggcaggtcgt cttcgacccc
     5341 gacgaggccg aacaactcgc ccgcgacggc tcgaaggtcg tcctcgtccg gcaggagacc
     5401 agccccgacg acttccacgg catggtcggc gcgcaggcca tcgtcaccgc ccgcggcggg
     5461 atgacgagcc acgccgccgt cgtggcgcgc ggcatgggca agtcgtgcgt gtgcggggcg
     5521 agcgcgctcg acatcgatta cggccagcag cagatgagcg tcaacgggtc ggtcgtgacg
     5581 aagggcgagt ggatcacggt ggacggctcg acggggcggg tcttcctcgg gcaggtgccg
     5641 accgtgcagc cgaagctcgg cgcggacttc cgcgagctga tgaagtgggc cgacgagttc
     5701 cgcgtcctgc gcgtgcgcgc caacgccgac acgccgcacg acgcggcggt ggcgcgcgac
     5761 ttcggcgccg agggcatcgg gctgtgccgc accgagcaca tgttcttcgg cgacgagcgt
     5821 ctggccgcga tgcgcgagat gattctggcg gacggcgtcg gcgcgcgcga gaaggcgctc
     5881 gaaaaactgc tcccgcttca gcgcaaagac ttcgtcggca tcttccgcgc gatggagggc
     5941 ctgcccgtca cgattcgtct gctcgacccg ccgctccacg agttcctgcc gaatctgcaa
     6001 gacctgctgg gcgagctggc cgagttgaag ttgaagctcc aacgcgccgg gagcatacag
     6061 gagatggaca gactgctcga cgatctcgac ggcaagcggc gcctgcttca tcaggtcgag
     6121 cgcacgcgcg agaacaaccc gatgctcggc caccgtggct gtcgtctggg tctggtctac
     6181 ccggaggtga cgcgcatgca ggctcgcgcc atcttcgagg ccgcgtgtca ggtgcagggc
     6241 gagggcacgg acatcaggcc cgagatcatg gtgccgctcg tctcgaccgc cgaagagctg
     6301 cgccaccagg agggcttgat ccgcgaggtc gcgcggcagg tgatgggcga gcacgggatg
     6361 acgattg
//
LOCUS       DBNGOH010000376         8220 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1157363, whole genome shotgun sequence.
ACCESSION   DBNGOH010000376 DBNGOH010000000
VERSION     DBNGOH010000376.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8220)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8220)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8220
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1157363"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..733
                     /locus_tag="AC159P_07455"
     CDS             <1..733
                     /locus_tag="AC159P_07455"
                     /inference="COORDINATES: protein motif:HMM:NF020893.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HIR3695328.1"
                     /translation="YWVVMDLNYVNDLMYTFGLPAIFVLTMLEGDITLLLAGVLAHGQ
                     AFGDWSFAQVLLVGTLGGVVSDQLAYALGRGGRRGVKQFRFYNAARSRIESLTGKFGI
                     LSMFVSKYTYGLRWAACTFYGVMKMPYLRFLALSFASCFVWVLSLAAAGYFFHSAIYN
                     LIGDFKKFPVILLCLVAAGIVGFYLVERFWLSKKVEEADPEKIQKFEQAAEVKLHEIR
                     EEIQEILPHPLARKEAPKKRNTEGD"
     gene            825..1832
                     /locus_tag="AC159P_07460"
     CDS             825..1832
                     /locus_tag="AC159P_07460"
                     /inference="COORDINATES: protein motif:HMM:NF012509.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GHMP family kinase ATP-binding protein"
                     /protein_id="HIR3695329.1"
                     /translation="MIIEATAPTRVDLAGGTIDIWPLYLFHPGATTVNFAVSMLARCR
                     IETRDDDRVILESRDRNLSFETTVGGVEDLVREERLELISKLVHFFKPETGFHLVAEG
                     QAPAGAGLGGSSALAIACIGALNRLVGDRYPERKFIVIAANVETTVIRVPAGFQDYYG
                     AFSGGANSIHFEPDGIRREALHVDGEQLERRIVICYTGEPRLSGINNWEMFKTHIDGD
                     PVIFDLFDQIGKTARRMRGALVAGDWQQVGEIMRAAYPNRKRLAPNITTPHMETLVER
                     ATDAGAVGAKVCGAGGGGCIAFLCEEGTREAVEQALVAEEGAEVLRWTFARDGLVVRE
                     G"
     gene            1863..2405
                     /locus_tag="AC159P_07465"
     CDS             1863..2405
                     /locus_tag="AC159P_07465"
                     /inference="COORDINATES: protein motif:HMM:NF014346.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR3695330.1"
                     /translation="MNQRAVRAIKSVACLFCFALAATALVGSGEISWGAAQAVVGQNQ
                     TTPQEAKPFDQEAALAELRKRIAGQENKPAEEVFKNIQLLKGVPAGRLLRVMEMGYAR
                     SLGVNCTHCHVAGEWEKEDRPTKQIAREMAAMVRVINEEQLKKIRNLKSTTPVVNCTT
                     CHRGQVKPALNLPEGVGSRQ"
     gene            complement(2620..3753)
                     /locus_tag="AC159P_07470"
     CDS             complement(2620..3753)
                     /locus_tag="AC159P_07470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695331.1"
                     /translation="MSTEASETKNEQTAKQAPTEQQESKAGVPKRFAVGQTVCNQKNE
                     KGKLCNGHLKERNTGHPAEVHLRGDDALHQCQTCGTLYMGPPLGHVRDSRKQSRYVER
                     DLAAILRAAGGTLPAYTYPVPEWRRASLPASAPAAKPTAPVGTPAEAPTPPAAAPPKP
                     APAARAEARPAPTESAANTTPPPASSAAPEGETPQQKRARIIAELNAKYAGKTAPPRK
                     KKADEETAAAPSAEAAPAPSAQATTGESAPAADASAPIVDSSAEADRHRPAAEAQAAT
                     GQASAAPLSPAAATASPAAGAETVADLAPEASTELTPGAPKADAPAAAEPAPEKRKAA
                     GQRNRGPRELPPHLRPPEGETHEEKIARLTAFVEEAKRKAGKL"
     gene            3991..4431
                     /locus_tag="AC159P_07475"
     CDS             3991..4431
                     /locus_tag="AC159P_07475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695332.1"
                     /translation="MADEKTTKPTIETVLERMAAQEERMNERMAAQEGRIMKALDDRL
                     GASEGRVMKALDDRLGASESRMNERMDGLEGRMMKALDERVGALDERVGGLKDYVEDR
                     LDRIEGVVSKTRGEMLETRADFREMRTEFRELREQIKEHFPASN"
     gene            4480..4944
                     /locus_tag="AC159P_07480"
     CDS             4480..4944
                     /locus_tag="AC159P_07480"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR3695333.1"
                     /translation="MLKRLLAVVWVNTPGFVRRWGVRLTQPRFTVTAGAVVCDERGRV
                     LLLRHVFRRGSGWGIPGGFLARGEQPDEAVRRELREETGLALEEVELAFVRTLARPHQ
                     VEVIFRCRMRAAALEGREDSLEIDRAEWFEREALPAGIGDDQRRLIARALGD"
     gene            5085..5711
                     /locus_tag="AC159P_07485"
     CDS             5085..5711
                     /locus_tag="AC159P_07485"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0046132 - pyrimidine ribonucleoside
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HIR3695334.1"
                     /translation="MPQLIPPQEEVMEILRKTGAYREGHFVYPSGQHTPHYFQMPLAF
                     RYYDTARVLAVALSRKFRTQKEISSALPKVAVISPGPGGIPVAFGVREALSAEQIYWA
                     EREAGGKRMFRQYVNVGEVNPCVIVDDISRTGGAIDETVEIVRQMGTRVIGCGTIVRF
                     TGAPDRLEVPNDDPIPIVSLIEFDAKWYDNAEDCAECKKGVPEEHVRF"
     gene            complement(5824..6438)
                     /gene="recR"
                     /locus_tag="AC159P_07490"
     CDS             complement(5824..6438)
                     /gene="recR"
                     /locus_tag="AC159P_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HIR3695335.1"
                     /translation="MLEYSEPVTKLIDEFKRLPGIGQKSAQRLAFHVLRMPEADVERF
                     VEALAEVKRKIVMCSVCNNLTDVEPCRFCSSTARDRSIICVVEEPYNLVAVEKTRSYH
                     GLYHVLHGSLSPIRGMGPDDLRMQNLLPRLRPENNDGVEVREVILATNPNTEGEATAN
                     YISRLLKPLGVRTTRIAMGMPVGSDLEYVDEVTMDKALANRHDI"
     gene            complement(6577..6882)
                     /locus_tag="AC159P_07495"
     CDS             complement(6577..6882)
                     /locus_tag="AC159P_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HIR3695336.1"
                     /translation="MKFPGGMNIQQMMKQAQAMQEKMAKEMEEMVVDATVGGGVVTVK
                     MRGTKEVESIRIDPEAFKEGDVEMLQDMIVAALNEAGRKIDEAMKSKLGGMLPPGLF"
     gene            7073..7660
                     /gene="msrA"
                     /locus_tag="AC159P_07500"
     CDS             7073..7660
                     /gene="msrA"
                     /locus_tag="AC159P_07500"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 - L-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HIR3695337.1"
                     /translation="MSEDRNANANAEGAGRGVTEVATLGGGCFWCVEAVFDELRGVSS
                     VESGYAGGRTANPTYEQVCSGATGHAEVVRVTFDPREVSFKEILEVFFTAHDPTTLNR
                     QGADVGTQYRSVIFYHDERQREVADETIRELDEANIWDGPIVTEVAPLSNYYEAEEYH
                     QEYFARNPGQGYCRAVVAPKVSKFRQRFRDRLKTN"
     gene            7740..>8220
                     /locus_tag="AC159P_07505"
     CDS             7740..>8220
                     /locus_tag="AC159P_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HIR3695338.1"
                     /translation="MSKRYVIKQPAGAQLPDRLARYREELNEEQFAVATAPAGAALVI
                     AGAGSGKTRAITYRVAYLVEQGVAPARVMLATFTNRAAREMLRRVEQLTGGSADVARR
                     VWGGTFHRVANLVLRRHAESIGYSSNYTILDAEDAKDFISVCIDEAGVDTRARRFPKA
                     "
BASE COUNT         1385 a         2609 c         2807 g         1419 t
ORIGIN      
        1 gtactgggta gttatggatt taaactacgt caacgacctg atgtacacgt tcgggctgcc
       61 ggccatcttc gtgctgacga tgctcgaagg cgacatcacg ttgctgctgg cgggcgtgct
      121 cgcgcacggg caggcgttcg gcgattggag cttcgcgcag gtcttgctgg tggggacgct
      181 gggcggcgtg gtgagcgacc agttggcgta cgcgctcggg cgcggcgggc ggaggggcgt
      241 caagcagttc cgcttctaca acgcggcgcg ctcgcgcatc gagagtctga ccggcaagtt
      301 cggaatcctc tcgatgttcg tctcgaagta cacgtacggc ctgaggtggg cggcgtgcac
      361 gttctacggc gtgatgaaga tgccgtacct gcgcttcctg gcgctgtcgt tcgcgagctg
      421 cttcgtgtgg gtcttgagcc tcgccgccgc cgggtacttc ttccatagcg cgatctacaa
      481 cctgatcggc gacttcaaga agttccccgt catcctgctc tgcctcgtcg ccgccgggat
      541 cgtcggtttc tacctcgtcg agcgcttctg gctctcgaag aaggtcgagg aggccgaccc
      601 cgaaaagatt cagaagttcg agcaggccgc cgaggtcaag ctccacgaga tcagggaaga
      661 gatacaggaa atcctgcccc acccgctcgc gcgcaaagaa gcgccgaaga agcgcaatac
      721 cgaaggagac tgaaagaaag gatgaagtcg gaacgcagaa ggatgaatcg aaaagctttt
      781 gtctttattc atcattctgc gttccgactt catcgtttgt ctttatgatt atcgaagcca
      841 ccgccccgac acgcgttgat ttggccggag ggaccatcga catctggccg ctgtacctgt
      901 ttcaccccgg cgcgacgacc gtgaacttcg ccgtctcgat gctggcgcgc tgtcgcatcg
      961 agacgcgcga cgacgaccgc gtgattttgg agtcgcgcga ccgcaacctc tctttcgaga
     1021 cgacggtcgg cggggtcgaa gacctcgtcc gcgaagagcg gctggaactc atctcgaagc
     1081 tcgtccactt cttcaagccg gagaccggct tccacctcgt cgccgaaggg caggccccgg
     1141 cgggcgctgg gctaggcggt tcgagcgcgc tcgccatcgc gtgcatcggc gctttgaacc
     1201 ggctcgtcgg cgaccgctac ccggagcgca agttcatcgt catcgcggcc aacgtcgaga
     1261 cgacggtgat ccgcgtgccc gcgggctttc aggactacta cggcgcgttc tccggcggcg
     1321 ccaactcgat ccacttcgaa ccggacggca tccggcgcga ggcgctccac gtggacggcg
     1381 agcagctcga acgccgcatc gtcatctgct acacgggcga gccgcgtctg tcgggcatca
     1441 acaactggga gatgttcaag acccacatcg acggcgaccc cgtcatcttc gacctgttcg
     1501 accagatcgg gaagacggcg cggcggatgc gcggcgcgct cgtcgcgggc gactggcagc
     1561 aggtgggcga gatcatgcgc gcggcctacc cgaaccgcaa acggctcgcg cccaacatca
     1621 cgacgccgca catggagacg ctggtcgagc gcgcgaccga tgcgggcgcg gtcggcgcga
     1681 aggtctgcgg cgcgggcggc ggcggctgca tcgccttcct ctgcgaagag ggcacgcgcg
     1741 aggcggtcga gcaggcgctc gtcgccgaag agggcgccga agtgctccgc tggacgttcg
     1801 cgcgcgacgg cctcgtcgtc cgcgagggct gagccgaagt tcggcacgaa aggacgggac
     1861 gtatgaacca gcgagccgtg cgcgctatca agtcggtcgc gtgtctcttc tgcttcgcgc
     1921 tcgccgcgac ggccctggtc gggtcgggag agatttcgtg gggcgcggcg caggccgtcg
     1981 tcggtcagaa tcagacgacg ccgcaggagg cgaagccgtt cgatcaggag gcggcgctcg
     2041 ccgagcttcg caagcgcatc gccgggcagg agaacaagcc cgcggaggaa gtcttcaaga
     2101 acatccaact gctgaagggc gtgcccgccg gtcggctgct gcgggtgatg gagatgggct
     2161 acgcgcgctc gctcggcgtc aactgcacgc actgccacgt cgcaggggag tgggagaagg
     2221 aagacaggcc gaccaagcag atcgcccgcg agatggccgc catggtgcgc gtcatcaacg
     2281 aagagcaact gaagaagatc aggaacctga agagcacgac gcccgtcgtc aactgcacga
     2341 cctgccaccg cggacaagta aagcccgccc tcaatctgcc cgaaggagta ggcagtaggc
     2401 agtaggcaga caagacagaa gacaagagac agtagacgga agaggcttgg gagttgaagt
     2461 cccaagcctc ttcttcgtca tgcagtaaaa gtgaagaggc ttgagatcga atgtctcaag
     2521 cctcttgctt tacagcttga agagtgatga ggtttgtgcc tttacctctc aagcctctgt
     2581 gctcttctgt cttgactgcc tactgcccgc tgcctactgc tacagctttc ccgccttgcg
     2641 cttggcctct tcgacgaagg cggtgaggcg cgcgatcttc tcttcgtgcg tctcgccttc
     2701 gggcggtcgg aggtgcgggg gcaactcacg cgggccacgg ttccgctggc cggcggcctt
     2761 gcgcttttcg ggcgcgggtt cggcggcggc gggcgcgtcg gccttcgggg cgccgggcgt
     2821 gagttcggtc gaggcttcgg gggcgaggtc ggccaccgtc tcggcgccgg cggcgggcga
     2881 ggccgtcgcg gcggcgggac tcagaggcgc ggcggaagcc tgaccggtag cggcctgcgc
     2941 ctcggcggcg gggcggtggc ggtcggcctc cgcggacgaa tcgacgatcg gcgcggacgc
     3001 gtcggcggca ggcgcggact cacccgtcgt cgcctgtgcc gaaggcgcgg gggcggcctc
     3061 tgccgaaggc gcggcggcag tctcttcgtc cgcctttttc tttcgcggcg gcgcggtctt
     3121 gcccgcgtac ttcgcgttga gttcggcaat gatgcgcgcg cgcttctgct gcggcgtctc
     3181 gccttcgggg gcggcggacg aagcgggcgg aggcgtcgtg ttcgcggcgg actcggtcgg
     3241 cgcgggacgt gcttcggcgc gcgcggcggg cgcgggctta ggcggcgcgg cggcgggcgg
     3301 tgtcggtgcc tccgcgggag tgccgacggg ggctgtcggt ttcgcggcgg gcgcgctcgc
     3361 gggcagggaa gcgcgtcgcc actcgggcac ggggtaggtg tacgcgggga gcgtgccgcc
     3421 cgcggcgcgg aggatggcgg cgaggtcgcg ctcgacgtag cggctctgct tgcgcgagtc
     3481 gcggacgtgg ccgagcggag gccccatgta gagcgtgccg cacgtctgac actggtgcag
     3541 cgcgtcgtcg ccgcgcaggt ggacttcggc agggtgcccc gtgttgcgct ccttgaggtg
     3601 gccgttgcag agcttgccct tctcgttctt ctggttgcac acggtctgcc ccacggcgaa
     3661 acgcttgggc acgcccgcct tcgactcctg ctgctcggtc ggggcctgct tcgccgtctg
     3721 ctcgttcttc gtctcggacg cttcggttga catcggctgt ggtcggaatc ctttgttcga
     3781 ttcgtgctga aaacgcggga cgggttgact cctaaagacg tgccagcatc ttacggacaa
     3841 gccgcgggag cgtcaacaaa aagagcacgc gcgccgcgac cttgcaaccc ctgttgcagc
     3901 gatcaagtgc gctgacaaaa tcaccgcacg gtggtataca tgttcgcagc ggttcaaaat
     3961 cctcatacga cttcagcgga ggcgcgagcg atggccgacg agaaaacgac gaagcccacc
     4021 attgaaaccg tccttgaacg catggccgca caggaagagc gcatgaacga gcgcatggcc
     4081 gcgcaggaag gccgcatcat gaaggcactc gacgaccgcc tcggcgcctc agaagggcgc
     4141 gtcatgaagg ctctcgacga ccgcctcggc gcgtccgaga gtcgcatgaa cgagcgcatg
     4201 gacggactcg aaggacgcat gatgaaggcg ctcgatgaac gcgtcggcgc acttgacgag
     4261 cgcgtcggcg ggctgaagga ttatgtcgag gacaggctcg accgcatcga gggtgtggta
     4321 agcaagacgc gcggcgagat gttggagacg cgcgcggact tccgcgagat gcggacggag
     4381 ttccgcgagc tgcgcgaaca gatcaaagag cactttcccg cttcgaactg acggacgcct
     4441 cacgcccttc gtgcattttt gttgtatgct aggcggccaa tgttgaagag gctgctggcg
     4501 gtcgtgtggg tgaacacgcc ggggttcgtg cggcggtggg gcgtgcggct gacgcagccg
     4561 cgcttcacgg tgacggcggg cgccgtggtg tgcgacgagc gcgggcgcgt gctgctgctg
     4621 cgccacgtct tccgcagggg gagcgggtgg ggcatcccgg gcggcttcct cgcgcgcggc
     4681 gagcagcccg acgaggccgt ccgccgcgag ctgcgcgagg agaccggcct cgcgctcgaa
     4741 gaggtggagc tggccttcgt ccgcacgctc gcgcgcccgc accaggtcga agtcatcttc
     4801 cgctgccgca tgcgggccgc cgcgctcgaa gggcgcgagg acagtctgga gatcgaccgc
     4861 gccgagtggt tcgagcgcga ggcgctgccc gcaggtatcg gcgacgacca gcgccgtctc
     4921 atcgcgcgcg ccctcggcga ctagcgccgg gctgcccgct tggtgtatat gctctgaaaa
     4981 acagagaggc cggaggtcag aggtcggagg tcagtgaaga actgtcttgt ccgtcctccg
     5041 acctctgacc tccgacctct gaccactagg agaagaccga catgatgccg caactgatcc
     5101 ctccgcagga ggaggtgatg gagatactga ggaagacggg cgcgtaccgc gagggccact
     5161 tcgtctaccc ttcgggccag cacacgccgc actacttcca gatgccgctc gccttccgct
     5221 actacgacac ggcgcgcgtg ctggcggtcg cgctctccag aaagttccgc acgcagaagg
     5281 agatttcgag cgcgctcccg aaggtcgccg tcatcagccc cggccccggc ggcatccccg
     5341 tcgccttcgg cgtgcgcgag gcgctgagcg ccgagcagat ttactgggcc gagcgcgagg
     5401 ccggcggcaa gcggatgttc cgccagtacg tcaacgtcgg cgaggtcaac ccctgcgtca
     5461 tcgtggacga catctcgcgc acgggcggcg ccatcgacga gacggtcgag atcgtccgcc
     5521 agatgggcac ccgcgtcatc ggctgcggca ccatcgtccg cttcaccggc gcccccgacc
     5581 ggctcgaagt ccccaacgac gaccccatcc ccatcgtctc gctcatcgag ttcgacgcca
     5641 agtggtacga caacgccgaa gactgcgccg agtgcaagaa gggcgtcccc gaagagcacg
     5701 tccgcttctg aaaagcagca ggcagcaggc agtaaaaaca aatgaaggga gctgcttggg
     5761 actttacctc tcaagcccat tcgcccttcc gtcttgactg cctgctgcct gctgcctgct
     5821 gctttatatg tcgtgacggt tggcgagcgc cttgtccatc gtgacctcgt ccacgtattc
     5881 gaggtcggag ccgacgggca tgcccatcgc gatgcgggtg gtgcggacgc ccagtggttt
     5941 gaggaggcgg ctgatgtagt tggcggtggc ttcgccctct gtgttggggt tggtggcgag
     6001 gatgacttcg cgcacctcga cgccgtcgtt gttctcgggc cggaggcgcg gcagcaggtt
     6061 ctgcatccgc aggtcgtccg gccccatccc gcggatcggc gagagcgagc cgtgcagcac
     6121 gtggtagagg ccgtggtagc ttctggtctt ctcgacggcg acgaggttgt acggctcttc
     6181 gacgacgcag atgatcgagc ggtcgcgcgc ggtggacgag cagaagcggc acggctccac
     6241 gtccgtcagg ttgttgcaga ccgagcacat cacgatcttc cgcttcacct ccgccagcgc
     6301 ctcgacgaac cgctccacgt ccgcctccgg catccgcagc acgtgaaacg ccagacgctg
     6361 cgccgacttc tgcccgatgc ccggcagccg cttgaattcg tcgatcagct tggtgactgg
     6421 ttcggaatac tcaagcatgg ctcgattgcg gaatgcggaa tgcggattgc ggattagcag
     6481 aagcggcgaa gattgatgtg agcagcactc tcgtcattga agacagcggc tccttcatca
     6541 accaccaatc cgaaatccgc attccgcaat ccagaattag aagagtcccg gcgggagcat
     6601 gccgccgagc ttggacttca tcgcctcgtc gatcttgcgc ccggcctcgt tgagggcggc
     6661 gacgatcatg tcctggagca tctccacgtc gccctccttg aaggcttcgg ggtcgatccg
     6721 gatggactcg acctccttcg tgccgcgcat cttgacggtc acgacgccgc cgccgacggt
     6781 ggcgtccacg accatctcct ccatctcctt ggccatcttc tcctgcatgg cctgcgcctg
     6841 cttcatcatc tgctggatgt tcatcccgcc cggaaatttc atcgcccttc aacctctccg
     6901 tcgtatgtga tttcggcctg ccgctcatcc tagcttcccg tcggcgcgtt tgcaacgtgc
     6961 gatagcgccg cccggcgcga gtgttgtacc ctttcggcgg cagcaaggtt cacgcgaaat
     7021 ttttacgaca tacggcgccg cggctggggc gagcggcggg agggagcgcg aagtgagcga
     7081 ggacaggaac gcgaatgcga acgcggaggg cgcggggcgc ggtgtgaccg aagtggcgac
     7141 gctcggcggc ggctgcttct ggtgcgtcga ggcggtgttc gacgagctgc gcggcgtctc
     7201 gtcggtcgag tcgggctacg cgggcgggcg caccgcgaac ccgacctacg agcaggtctg
     7261 ctccggggcg acgggccacg ccgaggtcgt gcgcgtcacg ttcgacccgc gcgaggtctc
     7321 tttcaaagag attctcgaag tcttcttcac ggcgcacgac ccgacgaccc tcaaccggca
     7381 gggcgccgac gtaggcacgc agtaccgctc cgtcatcttc taccacgacg agcggcagcg
     7441 cgaagtggcc gacgagacca tccgcgaact cgacgaggcg aacatctggg acggcccaat
     7501 cgtcaccgag gtcgcgccgc tctcgaacta ctacgaggcc gaggagtacc accaggaata
     7561 cttcgcgcgc aaccccggtc agggctactg ccgcgccgtc gtcgcgccca aggtgtccaa
     7621 gttccgccag cgcttccgcg accggctgaa aaccaactga caaaagtact gagtactgag
     7681 tactgagtac tgagtgaaga caaattgctt ctacccagta ctcagtactt cgatttcctg
     7741 tgagcaagcg ctacgtcatc aagcagcccg cgggcgcgca gttgccggac cggctggcgc
     7801 gctaccgcga ggagttgaac gaggagcagt tcgcggtggc gacggccccg gcgggcgcgg
     7861 cgctcgtcat cgcgggcgca ggcagcggga agacgcgcgc catcacgtac cgcgtcgctt
     7921 acttggtcga gcagggcgtg gcgcccgcgc gcgtgatgct ggcgacgttc acgaatcgcg
     7981 ccgcgcgcga gatgctgcgc cgcgtcgagc agttgacggg cggctccgcg gacgtggcgc
     8041 gccgcgtctg gggcggcacc ttccaccgcg tcgccaacct cgtgctgcgg cgccacgccg
     8101 aatcaatcgg ctactcctcg aactacacca tcctcgacgc cgaggacgcg aaggacttca
     8161 tctcggtctg catcgacgag gcgggcgtgg acacgcgcgc gcgccgcttc ccgaaagccg
//
LOCUS       DBNGOH010000377         6250 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1340279, whole genome shotgun sequence.
ACCESSION   DBNGOH010000377 DBNGOH010000000
VERSION     DBNGOH010000377.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6250)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6250)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6250
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1340279"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1730
                     /locus_tag="AC159P_07510"
     CDS             <1..1730
                     /locus_tag="AC159P_07510"
                     /inference="COORDINATES: protein motif:HMM:NF013283.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA];
                     GO_process: GO:0071709 - membrane assembly [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="BamA/OMP85 family outer membrane protein"
                     /protein_id="HIR3695339.1"
                     /translation="RIRAFYSRNGFLEADVDLDVVELPPARPGGDERVRLIYNVAEAD
                     RVYINRIFVNGNVLTKREAITDAIPLREGTVLRGDDLAESERILLNTTDAFRQVIIRT
                     EAAGETSGGFKNRDELIHVEERKRITTDYIVGFSTDNGPLFGFEIRNTNLFGQLRQGA
                     FRSRFSRRNQLVRLEYFDPRFRRYGEKEFAPLTVSLQYQRDTSVTRFFRSTLDRGGSQ
                     GVVQRFDMEGNLIDEFGESIKEPSINRLTFNLETQRDLELELSPTGAVRKRSTIFLRY
                     NYEDVRLFNIGSLLIAPVLRPDQVVRLSRFGVSFARDTRDRQFDPTRGDFVTVDYALA
                     LKPLGGSLSFNKLLTSYRRYYRINRVRETVLAASVQFGLAKIINPPDRNEDGVINEAD
                     KRLPISERFFSGGSTTLRGFGFEEAGPRIVVPDCTFGSVPPALPIVSPTPCGEFRNQE
                     GELITLNPFTVPIGGNAMAVVNLEARVGLTRDLQAVPFYDGGNVFESVRDIFGGTDDP
                     ARNPNFRAKWAHTVGLGFRFKTPFGSLAVDYGYLLNPPEFVIPQNGGGTAIHRLKNSQ
                     LHFRFGQTF"
     gene            1937..3733
                     /locus_tag="AC159P_07515"
     CDS             1937..3733
                     /locus_tag="AC159P_07515"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HIR3695340.1"
                     /translation="MRLLVLHDSRPEAARVQHAVADNYRHGARLLAAGGVPAFFSKSS
                     PLADPDTLGHPPGYPVLLAVVSRLSVTSEVGDVKSSEDAARLVQLAADAFAAALLFLL
                     VAELLPFAAGVVAGALVALAPQFTWNSVLPLPDTLAAVLVLLAVYLLARAARRPRLMT
                     VVLAGASIGLSCWLRANALLLAPLLAVAVFLLFERERRKRYALALVAGAVLVVAPLTI
                     RNALVFGRFIPLSLGAGQTLLEGIADYDDEGRFGIPRTDLGIMRQEAEGAGRPDYAAT
                     LFGPDAVARERARLRRGLSVITSHPLWFGGVMARRAVSMLRLERARLVSPRPPVTHAL
                     EDVDRAAVVWSRSPAELLRDGDRVTTGAEVLPADDGAALRVVGDGSKFGRQFAAAPVA
                     VEAGTDYAFSVPVRVESGRMSVEVSRVGEDAAHVSAIVETEEGKSAAEQPARVVPLPF
                     ANAGAGRVRLLLANAPSEPPRPVAEIVGEVRLHRLGPASLLWTRYPRVVVNGLQRLFV
                     TAVMLPLALAGLALAARERRRTALVILLAVPVYYMCVQSALHTEYRYVLAIHYFLFAL
                     AAFALYRAGLLATEAVRGLRRARRLMKAEGEV"
     gene            3730..4404
                     /locus_tag="AC159P_07520"
     CDS             3730..4404
                     /locus_tag="AC159P_07520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HIR3695341.1"
                     /translation="MKRVLCSVLLVLALWGGVRGQERAAAARGGEAVDRMVAVINGRE
                     LITFTDLLWQLALEPRVPLDNPRQEDINRALDLVINQRLIAQEAEKLPTVAPTEDEVR
                     AELARLINFFPSQAAFYERLNRVGLGESSARLREIVEGRVRINKYIDFRFRSFTVVTP
                     QEVAAYYRDVYVPRFRRQSPGRIVPTAEEVYREIERELTDSKIESDTDEFLEEARSSA
                     EITILD"
     gene            4603..5262
                     /locus_tag="AC159P_07525"
     CDS             4603..5262
                     /locus_tag="AC159P_07525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695342.1"
                     /translation="MRRLTPIVLSLALIASCAAAATAQRQRPRPQPASSPAKTTTPAE
                     QGATILVVPDAEDGTTTVRLETFAVEHDKAAKSQLLLAAHFKQGANGAAPAFVHLTLF
                     SHAPRCRFPPQADLELALDGAPVVLKFQPDSSTTATTASLEVVTVFVPKAEGVLWVQR
                     ETAEGGDCSESLAATLSPQTFARMAAARAVALKIGTATFYLKPNALAALRDLGGKMKK
                     Q"
     gene            5417..6193
                     /locus_tag="AC159P_07530"
     CDS             5417..6193
                     /locus_tag="AC159P_07530"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013862.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HIR3695343.1"
                     /translation="MKRQGVERPRVGLALSGGAARGMAHVGVLKVFKEHGVPVDCVAG
                     TSAGALVGAAFAAGMPVEELEEIGRRLRWRDFGRMTLSRLGAQSNARMEEYVRASFPV
                     SRFEELPIPFAAVATDLHTGAAVVMRDRGDVAFAVRASCAVPGWYVPVVDEHGRQLVD
                     GGLVANIPTAAVRALGAEVVVAVDVNFEGAKFMGSPTSVVGVLLQSILVVQKTAASHQ
                     RHAADIVLSPRGGHIRWDEMTRAAERIAAAYEDLLSTTVR"
BASE COUNT          949 a         2214 c         2070 g         1017 t
ORIGIN      
        1 agcgcatccg cgccttctac tcgcgcaacg gcttcctcga agccgatgtt gacctcgacg
       61 tggtcgagct gccgcccgcc cgaccgggcg gcgacgagcg cgtccggctc atctacaacg
      121 tggccgaggc cgaccgggtc tacatcaatc gtatctttgt caacggcaac gtgctgacca
      181 agcgcgaggc catcaccgac gccatccctt tgcgcgaggg aacggtgctg cgcggcgacg
      241 atctggccga gagcgagcgc atcctcctga acaccacgga cgccttccgg caggtcatca
      301 tccgcaccga agccgcgggc gagacctcgg gcggcttcaa gaatcgcgac gagctcatcc
      361 acgtggagga gcgcaagcga atcacgaccg actacatcgt cggcttctcg accgacaacg
      421 gcccgctctt cggcttcgag attcgcaaca cgaatctctt cggccagctc cggcagggcg
      481 ccttccgctc gcgcttcagc cgccgcaacc agctcgtccg cctcgaatac ttcgacccgc
      541 gcttccgccg ctacggcgag aaggagttcg cgccgctcac cgtctctttg cagtaccagc
      601 gcgacacgtc cgtcacgcgc ttcttccgct cgacgctcga ccgcggcggc agtcagggcg
      661 tcgtccagcg cttcgacatg gaaggcaacc tcatcgacga gttcggcgag agcatcaaag
      721 agccttcgat caaccgcctc accttcaacc tcgaaacgca gcgcgacctc gaactcgaac
      781 tcagccccac gggcgcggtc cgcaagcgca gcacgatctt cctgcgctac aactacgagg
      841 acgtgcgcct cttcaacatc gggagcctgc tcatcgcgcc cgtcctccgg cccgatcagg
      901 tggtgcgcct ctcgcggttc ggcgtctcgt tcgcacgcga cacgcgcgac cggcagttcg
      961 acccgacgcg cggcgacttc gtcaccgtcg attacgcgct cgccctgaaa cctctgggcg
     1021 gcagcctctc cttcaacaag ctcttgacga gctaccgccg ctactaccgc atcaaccgcg
     1081 tgcgcgagac cgtgctcgcg gcgagcgtgc agttcggcct cgccaagatc atcaacccgc
     1141 ccgaccgcaa cgaggacggc gtgattaacg aggccgacaa gcgactcccc atcagcgagc
     1201 gcttcttctc cggcggctcg accacgctgc gcggcttcgg cttcgaggag gcggggccgc
     1261 gcatcgtcgt ccccgactgc accttcggca gcgtgccgcc cgcgctcccc atcgtcagcc
     1321 ccacgccctg cggcgagttc cgcaaccagg agggcgaact catcacgctc aaccccttca
     1381 ccgtgcccat cggcggcaac gcgatggcgg tcgtcaacct cgaagcgcgc gtcggcctca
     1441 cgcgcgactt gcaggccgtg cccttctacg acggcggcaa cgtcttcgag agcgtccgcg
     1501 acatcttcgg cggcaccgac gaccccgccc gcaaccccaa cttccgcgcc aagtgggcgc
     1561 acaccgtcgg cctcggcttc cgcttcaaga cgcccttcgg ctcgctcgcc gtggactacg
     1621 gctacctcct gaacccgccc gagttcgtca tcccccagaa cggcggcggc acggccatcc
     1681 accgcctgaa gaacagccag ctccacttcc gcttcgggca aacgttctga agaaggcgaa
     1741 aggaaaaagg taaaaggcaa aagtgaggac agggttgacg acgaaagcgg agggcgtaaa
     1801 ggcgaccggc gacggcccgc gacccgcgac ccgcccgccg gccgaccgcc gttcacttta
     1861 cctcccgaca accgccgacc gcccgcactt tcaccttttc actttcgcct ttttactttt
     1921 cctgctcgcg ctcggcgtgc gtctcctcgt gctgcacgac tcgcgtccgg aggcggcgcg
     1981 cgtgcagcac gcggtggcgg acaactaccg gcacggcgcg cggctgctcg ccgcaggggg
     2041 cgtcccggcc ttcttcagca agtcctcgcc gctcgcagac ccagacacgc tcggccaccc
     2101 gccgggttat cccgtgctgc tcgccgtggt ctcgcgactg tcggtcactt cggaggtggg
     2161 ggacgtaaag agttcggaag acgcagcgcg gctcgtccag ctcgcggcgg acgccttcgc
     2221 cgccgcgctc ctgttcctgc tcgttgcgga gctgctgcct ttcgccgcgg gcgtcgtcgc
     2281 cggggcgctc gtcgcgctcg cgccgcagtt cacttggaac tccgtgttgc ctttgcccga
     2341 cacgctcgcg gcggtgctcg tgctgctcgc ggtctacctg ctcgcgcgtg ccgcgcgccg
     2401 accgcggctg atgacagtcg tgctggccgg agcgtcaatc ggcctgtcgt gctggctgag
     2461 ggcgaacgcg ctgctgctcg cgccgctgct cgcggtcgct gtttttttat tgttcgagcg
     2521 tgaacggcgc aagcgttacg cgttggcatt ggtcgcgggc gcggtgctcg tcgtcgcgcc
     2581 tttgacgata aggaacgcgc tggtgttcgg tcgtttcatc ccgctctcgc tcggggcggg
     2641 gcagacgctg ctggaaggca tcgccgacta cgacgacgag gggcggttcg gcatcccgcg
     2701 cacggacctc ggcatcatgc ggcaggaggc cgaaggcgcg gggcggcccg actacgccgc
     2761 gacgctcttc ggccccgacg ccgtcgcgcg cgagcgggcg cgccttcgtc gcggcttgtc
     2821 cgtcatcacc tctcacccgc tctggttcgg cggcgtgatg gcgcggcgcg cggtctctat
     2881 gctccggctc gaacgcgcgc gcctcgtctc gccgcgaccg cccgtgacgc acgcgctcga
     2941 agacgtggat cgcgcggcgg tcgtctggtc gcggagtccc gcggagctac tgcgcgacgg
     3001 cgaccgcgtg acgacggggg cggaggtctt gccggcggac gacggcgcgg cgctgcgcgt
     3061 ggtcggcgac gggtcgaagt tcgggcggca gttcgccgcc gcgcccgtcg cggtcgaggc
     3121 cgggacggac tacgccttca gcgtgcccgt ccgcgtcgag agcgggcgga tgagtgtcga
     3181 ggtgtcgagg gtcggggagg acgcggcgca cgtctcggcc atcgtcgaga cggaagaagg
     3241 gaagagcgcc gccgagcagc ccgcgcgcgt cgtcccgctg cctttcgcca acgcgggcgc
     3301 gggccgcgtg aggctgttgc tggcgaacgc gccttcggag ccgccgcgcc cggtcgcgga
     3361 gatcgtcggc gaggtgcggc tccacaggct cggccccgcg tcgctgctct ggactcgtta
     3421 cccgcgagtc gtcgtcaacg gcctgcaaag actcttcgtc accgccgtca tgctcccgct
     3481 cgcgctcgcc ggcctcgccc tcgccgcgcg cgagaggcgc cggacggcgc tcgtcatcct
     3541 gctcgccgtg ccggtctact atatgtgcgt gcagtcggcg ctccacacgg agtatcgcta
     3601 cgtgctggcg attcattact tcctcttcgc gctcgcggcg ttcgcgcttt accgcgcggg
     3661 cctgttggcg acggaagcgg tgcgaggact ccggcgcgcg cgaaggttga tgaaagcgga
     3721 aggtgaagta tgaagagggt gctgtgctcg gtcctgctcg tcttggcgct ctggggtggt
     3781 gtgcgcgggc aggagcgcgc ggcggcggcg cgcggcggcg aggcggtgga caggatggtg
     3841 gcggtcatca acggcaggga gttgattacg ttcacggacc tgctgtggca gctcgcgctg
     3901 gagccgcggg tgccgctcga caacccgcgg caggaggaca tcaaccgcgc gctcgacctc
     3961 gtcatcaacc agcggctcat cgcgcaggag gccgagaagc tgccgacggt cgcgcccacg
     4021 gaggacgagg tcagggccga gctggcgcgg ctcatcaact tcttcccgtc gcaggcggcc
     4081 ttctacgagc ggctgaaccg cgtcgggttg ggcgagtcct ccgcccgcct gcgcgagatc
     4141 gtcgaggggc gcgtccgcat caacaagtac atcgacttcc gcttccgctc cttcaccgtc
     4201 gtcacgccgc aggaggtcgc ggcctactac cgcgacgtgt acgtcccgcg cttccgccgc
     4261 cagtcgcccg gtcgcatcgt gcccacagcc gaagaggtct acagggagat cgagcgcgag
     4321 ctgaccgaca gcaagatcga atccgacacc gacgaattcc tcgaagaggc gcgctcgtcc
     4381 gccgagatca cgatcctcga ttgagacttg gggctagagc tggaggctgg ttaacagcat
     4441 gatatcttcc aataacctcc ggcttctaac ctccggtctc aagcctcaag cctcaagcct
     4501 caagtctcta gcctcaagtc cctccttgcg gtgttataat cggcccccac ggacaaggcg
     4561 gtgacagctc tttacatctt catcagcaaa aggagcacac ggatgagacg cctgaccccc
     4621 atcgttttat ctctcgcctt gatcgcctcc tgcgccgccg cggcgaccgc gcagcggcag
     4681 aggccgcgac cacagcccgc gtcctccccg gcgaagacga cgacgcccgc cgagcaaggc
     4741 gccacgatcc tcgtcgtgcc cgacgccgaa gacggcacca ccaccgtcag gcttgagacg
     4801 ttcgcggtcg agcacgacaa ggccgcgaag tcgcagctcc tgctcgcggc tcatttcaaa
     4861 cagggtgcga acggcgccgc gcccgcgttc gtccacctga ccctgttctc gcacgcgccg
     4921 cgctgccgct tcccgccgca ggccgatctg gaactcgcgc tcgacggggc gcccgtcgtg
     4981 ctcaagttcc agcccgactc ttcgacgacg gcgacgacgg cctcgctgga agtcgtgacg
     5041 gtcttcgtcc cgaaggcgga gggtgtcctg tgggtgcagc gcgagaccgc cgaaggcggc
     5101 gactgctccg agtcgctcgc ggccaccctc tcgccgcaga ccttcgcgcg catggccgcc
     5161 gcccgcgccg tcgcactcaa aatcggcacg gccaccttct acctcaaacc gaacgccctc
     5221 gcggccctgc gcgacctcgg ggggaagatg aagaagcagt aggcagcagg cagcaggcag
     5281 taggcagtca aagaaagtac atcttaaaga ttatggtgaa ggtcgcgcgc ggttgtcatg
     5341 ctcgatcgtc gacagagtca acggttgact gcctgttgcc tactgcctgc tgcctactgc
     5401 tgctatgtgg ggacgagtga agcgacaagg ggtcgagagg ccgcgtgtgg ggctggcgct
     5461 gtcgggcggg gcggcgcgcg gcatggcgca cgtgggcgtg ctgaaggtct tcaaagagca
     5521 cggcgtcccc gtggactgcg tggcggggac gagcgcgggg gctttggtgg gcgcggcgtt
     5581 cgcggcgggg atgccggtcg aggagttgga ggagatcggc aggcgcctca ggtggcgcga
     5641 cttcgggcgg atgacgctct cgcggctcgg cgcgcagtcg aacgcgcgga tggaagagta
     5701 cgtccgcgcg agcttccccg tctcgcgctt cgaggagctg cccatcccct tcgcggcggt
     5761 cgccaccgac ctgcacacgg gcgcggcggt ggtcatgcgc gaccgcggcg acgtggcctt
     5821 cgccgtgcgc gccagttgcg cggtgcccgg ctggtacgtg cccgtcgtgg acgagcacgg
     5881 gagacagctc gtagacggcg gcctcgtcgc caacatcccg acggcggcgg tgcgcgctct
     5941 cggcgccgag gtggtcgtcg cggtggacgt gaacttcgag ggcgcgaagt tcatggggtc
     6001 gcccacgtcg gtcgtcggcg tgctgctgca atcgattctc gtcgtgcaga agaccgccgc
     6061 ctcgcaccag cgccacgcgg cggacatcgt cctcagcccg cgcggcggcc acatccgctg
     6121 ggacgagatg acgcgcgccg ccgagcggat cgccgcggcc tacgaggacc tgctctcgac
     6181 cactgtgcgg tagacgcgct ccgttgcggc ggcgcaggcg tcccacgtga acgcttgcgc
     6241 tcgccggagc
//
LOCUS       DBNGOH010000378         4716 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1890241, whole genome shotgun sequence.
ACCESSION   DBNGOH010000378 DBNGOH010000000
VERSION     DBNGOH010000378.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4716)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4716)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4716
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1890241"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..807
                     /locus_tag="AC159P_07535"
     CDS             <1..807
                     /locus_tag="AC159P_07535"
                     /inference="COORDINATES: protein motif:HMM:NF051698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HIR3695344.1"
                     /translation="FSKALGVTATDETGTEIDPIDARNRTYGILPFDRTHVINMSYTY
                     VLPQLARGSFDNKFARGVLNGWQMSGITTYSSGAPMYLRFGGDINNNVAFAAFGTNAF
                     TTTGRAAGGITPVFLRDPSIDGGSSQGDKILDIGALGIPNFPGSGPFQSPFYLRSPHR
                     WNHDVTFFKNFIFTETRKLQFRAAFFNLFNQAYPRWFGIGDNNNDINTTLNVTCNVRR
                     DNVPNGNGTNNNVCDPTGGFSFTQDTIDNFGRITRKRGHRVVELAVKFYF"
     gene            1020..3104
                     /locus_tag="AC159P_07540"
     CDS             1020..3104
                     /locus_tag="AC159P_07540"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HIR3695345.1"
                     /translation="MSPRQQDEETVRVGTSEVVVDVVVRDKKDRHVKDLTAADFEIHE
                     DGVRQQVQSFRLVTRGMTTALPAAAGRDDRPPVRETAPGPGAPAAGVVAIVFDRLSPD
                     ARARAGKAALSYVGAGPGDNDFIGVFAIDQSLRALQPFTADRQLVRRAVERAAGASPS
                     SYTSNTEEVRRLSERQEALARETGAATGTAERDAAGIAEEALTQMTARTLETYERLER
                     EQQGYATTNGLLAVVNAMGRLRGRKALIFLSEGVAIPTAVQDYFRAVIGNANRANVSI
                     YAVDAAGLRVESTTAETQRELQAIGARRQRQAASGLEDRSGQPMSRVLERNEDVLRLN
                     PESGLGQLADQTGGLLISQTNDIASKLRQVDDDLHTHYVLSYVPANRNYDGRFRQIAV
                     KLNRAGYDVRARKGYYAINATDASPVLAYEAPALAALGGARAPSAFPVYADGFGFPEA
                     GRPDLVAVVAEIPHGGIDFSHDAEKKIYRSDFSVVALIRDEARQVVRKLSRQYLSVVP
                     GDRIEAAKRGLTLFYRETDLPAGRYTLDVAVHDAVAGKTGVSSGSLEVSGGGEKLRMG
                     SLAILKRAEKLSPEEQKQNNPFHYGEVLVYPNLGEPIRKATAGQLAFFLTAYTARPSS
                     SPPKLMLEIKQRGRTLSRAQIDLPAPDAAGRIQFANALPIDKLQPGEYELSATATDGA
                     TSVTRVKRFVIQ"
     gene            complement(3163..4344)
                     /locus_tag="AC159P_07545"
     CDS             complement(3163..4344)
                     /locus_tag="AC159P_07545"
                     /inference="COORDINATES: protein motif:HMM:NF025890.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HIR3695346.1"
                     /translation="MKKVKAGVLVAFALGLSAWFGPSLLSYGQGQIQGPAPSSSRPWR
                     PNKPFKEAGYVGSEACAECHAGIAAKQHNTAMGNALETVAESRVLRSRPRFTFRLGPY
                     TYQITRQGERSIYSVTDGVNTISEPILYSFGQGKAGQTYVLRRGPSPAFYESRVSYYR
                     RLDGLDITLGYPPTAPSSLEEAFGRQISTDEARSCFACHSTASVSGRNLQLEHLSPGV
                     RCEACHGPGREHVAAMESGKFLDPRIFNPGKMSPDELTQDFCGSCHRSAEQVVEMNML
                     NINNVRFQPYRSFTSAGHDPNDKRLSCTACHDPHDNPRSDAASYDGNCLSCHQSAAAL
                     KSALTAKSERSKGRAAKACPVGDKNCVTCHMPKVELPGSHFKFTDHRIRIARPGEPFP
                     N"
     gene            complement(4402..>4716)
                     /locus_tag="AC159P_07550"
     CDS             complement(4402..>4716)
                     /locus_tag="AC159P_07550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3695347.1"
                     /translation="ALKLSPRNAVGHYILGRLLFDAGQNDRSIKELEAARSLAPLAPN
                     IHFALARAYTRANRKADADRARETFARLNKQAEEIDNKGFFTTGDAITDGAPGGGQTP
                     PP"
BASE COUNT          790 a         1576 c         1602 g          748 t
ORIGIN      
        1 ttctcgaagg cgttaggcgt cacggcgacc gacgaaaccg gtacggaaat agatcccatc
       61 gacgcgcgca acaggacata cggcatcctg ccgttcgacc ggactcacgt cattaacatg
      121 tcgtacacct acgtcctgcc gcaactggct cgggggtcgt tcgacaacaa gttcgcccgc
      181 ggcgtcctga acgggtggca gatgtccggc atcaccacct attcgagcgg cgcacccatg
      241 tacctgaggt tcgggggcga catcaacaat aacgtcgcgt tcgccgcttt cgggaccaac
      301 gcctttacca cgaccggacg cgcggcgggc ggcatcactc ccgtcttcct gcgcgacccg
      361 agcatcgacg gcgggagcag ccagggcgac aagatactcg acatcggcgc actcggcatc
      421 ccgaacttcc ccggttccgg gccgttccag tcacccttct acctgagaag cccgcaccgc
      481 tggaaccatg atgtgacgtt cttcaagaac ttcatcttca cggagaccag gaagctgcag
      541 ttccgcgcgg cattcttcaa cctcttcaac caggcgtacc cgcgttggtt cggtatcggt
      601 gacaataaca atgacatcaa caccaccctg aacgtcacgt gtaacgtgcg tcgggataac
      661 gttcccaacg gcaacggtac taacaacaac gtctgtgatc ctacgggcgg gttcagcttc
      721 acgcaggaca ccatcgacaa cttcggtcgg atcacgagga agcgcggcca ccgcgtggtc
      781 gagcttgccg tcaagttcta cttctaaggg ccgaccgagt ttgcttctga atctggcgcg
      841 gggggcggaa ggtgttgccg ccctcccgcg ccagacttgt gatgggcgag gcaggacggt
      901 gcccggcttc ccccgcggcc ttcgacactt tgccggacgc ttcgaccatg aaaacatacg
      961 ccgctctttt agcctgcgtg ttgtcgttag cccctctccg gggcttcgcc cagacccctg
     1021 tgtcgccgcg acagcaggac gaggagacgg tcagggtggg cacgagcgag gtggtcgttg
     1081 acgtggtggt cagggacaag aaggatcgcc acgtcaaaga ccttaccgcc gcagacttcg
     1141 aaattcatga ggacggcgtg cgtcagcagg tgcagtcatt ccgcctcgtc acgcgaggga
     1201 tgacgaccgc cctccccgcc gccgcgggga gagatgacag gccgccggtt cgggagaccg
     1261 cgccggggcc gggcgcgcct gcggcgggcg tggtcgctat cgtcttcgac cggctctcgc
     1321 ccgacgcccg cgcgcgcgcc ggcaaggcgg ccctcagcta cgtcggggcg gggccggggg
     1381 ataacgactt catcggggtc ttcgccatcg accagtcgct gcgcgcgctc cagccgttca
     1441 ccgccgaccg gcaactcgtc cgccgggccg tggagcgcgc cgccggggcg agtccttcga
     1501 gctacacctc gaacacggag gaggtgcgcc ggctgtcgga gcggcaagag gcgttggcga
     1561 gggagacggg ggcggcgacc ggaaccgcgg agcgcgacgc cgcgggcatc gccgaggagg
     1621 cgctcacgca gatgaccgcg cggacgctcg aaacctacga gcgtctggag cgcgagcagc
     1681 agggctacgc caccaccaac ggcctgctcg cggtggtcaa cgccatgggc cggctccggg
     1741 ggcgcaaggc gctgatcttc ctctccgagg gggtcgccat cccgaccgcc gtgcaggatt
     1801 acttccgcgc ggtcatcggc aacgccaacc gggcgaacgt cagcatctac gccgtggacg
     1861 cggcgggcct gcgcgtggag agcaccaccg ccgagacgca gcgcgagctt caggcgatag
     1921 gggcgcgcag acagcggcag gccgccagcg gcctcgaaga caggtcgggg cagccgatga
     1981 gcagggtgtt ggagcgcaac gaggacgtgc tccgcctgaa ccctgagagc gggctggggc
     2041 agctcgcaga ccagaccggc ggactcctca tcagccagac caacgacatc gcgtccaagc
     2101 tccggcaggt tgacgacgac ctgcacaccc actacgtcct cagctacgtc cccgcgaacc
     2161 ggaactacga cgggcgcttc cgccagatcg cggtcaagct caaccgcgcg ggctacgacg
     2221 tgcgggcgcg caagggctat tacgcgatca acgccaccga cgcgtcgccc gtactcgcct
     2281 acgaggcccc cgcgctggcc gcgctcggcg gcgcgcgcgc gccctcggcc ttccccgtct
     2341 acgccgacgg gttcggcttc cccgaagcgg gccggcccga cctcgtcgcg gtcgtcgccg
     2401 aaatcccgca cggcggcatc gacttttcgc acgacgcgga gaagaaaatc taccgctcgg
     2461 atttttccgt cgtggcgctg ataagggacg aggcgcggca ggtcgtgcgc aagctcagcc
     2521 gccagtacct ctcggtcgtg ccgggcgacc ggatcgaagc ggcgaagcgc ggcctcaccc
     2581 tcttctaccg cgagaccgac ctgcccgccg ggcgctacac cctcgacgtg gcggtccacg
     2641 acgccgtcgc cggcaagacg ggcgtgagca gcgggagcct tgaagtgagc ggcggtgggg
     2701 agaagctgcg catgggcagc ctcgccatcc tcaagcgcgc cgagaaactc tcccccgagg
     2761 agcagaagca gaacaacccc ttccactacg gcgaagttct cgtctacccg aacctcggtg
     2821 aacccatccg caaagcgacc gccgggcagc tcgccttctt cctgacggcg tacacggccc
     2881 ggccctcgtc atccccgccc aaactgatgt tggagatcaa gcagcgcggg cgcacgctca
     2941 gccgcgcgca gatagacctc ccggccccgg acgccgccgg gcgcatccag ttcgccaacg
     3001 ccctgcccat cgacaaactg caacccggag agtatgagct gtcggccacc gccacggacg
     3061 gcgccaccag cgtcacccgc gttaagcgat tcgttattca gtaagagcgg taatcggagt
     3121 gcctaacgaa gcggggttga agggtcggcg ggccgcggcg cgtcagttcg ggaacggctc
     3181 gccgggccgg gcgatgcgga tgcggtggtc cgtgaacttg aagtgcgagc cgggcagctc
     3241 gactttgggc atgtggcagg tcacgcagtt tttatccccg accggacacg ccttcgccgc
     3301 gcggcccttc gaccgttcgg acttggcggt gagcgcggac ttgagcgcgg cggcggactg
     3361 gtggcaggag aggcagttgc cgtcgtaaga ggccgcgtcg ctgcgcgggt tgtcgtgcgg
     3421 gtcgtggcag gccgtgcagc tcaggcgctt gtcgttcggg tcgtggccgg cgctggtgaa
     3481 agaccggtag ggctggaagc gcacgttgtt gatgttcagc atgttcatct cgacgacctg
     3541 ctcggcgctg cggtggcagg agccgcagaa gtcctgcgtc agttcgtcgg gactcatctt
     3601 gccggggttg aagatgcgcg ggtcgaggaa cttccccgac tccatcgccg cgacgtgctc
     3661 ccggcccggc ccgtgacacg cctcgcagcg gactccgggc gaaaggtgtt cgagttgcag
     3721 gttcctgccg ctcacggagg cggtcgagtg gcaggcgaag caactgcgtg cctcgtccgt
     3781 gctgatctgc cgcccgaacg cctcctccag cgaagacggg gccgtcgggg ggtagccgag
     3841 ggtgatgtcg aggccgtcca gccgcctgta gtagctgacg cggctctcgt agaaagcggg
     3901 gctggggccg cgccgcagca cgtaggtctg gccggccttg ccctgcccga acgagtaaag
     3961 gatgggttcg gagatggtgt tgacgccgtc ggtcacggag tagatgctcc gctccccctg
     4021 gcgcgtgatc tggtaggtgt aagggccgag ccggaacgtg aagcgggggc gtgacctcag
     4081 cacgcggctc tcggcgaccg tctccagcgc gttgcccatc gccgtgttgt gctgcttggc
     4141 cgcgattccg gcgtgacact cggcgcacgc ctcgctgccg acgtaccccg cctccttgaa
     4201 aggcttgttc gggcgccacg ggcgcgacga ggagggcgcg gggccttgta tctgcccctg
     4261 cccgtaagac aggagcgacg ggccgaacca ggccgagagg cccaacgcga aagccacgag
     4321 gacgccggcc tttactttct tcacggtgaa tcccatctgc cctttgagcg actgcgcccg
     4381 gaggtgaact cgtcgtcggc cttacggcgg aggcgtctgg cccccgccgg gtgcgccgtc
     4441 ggtgatcgcg tcgccggtcg tgaagaagcc cttgttgtct atctcctcgg cctgcttgtt
     4501 aaggcgcgcg aaggtctcgc gcgcgcggtc ggcgtcggcc ttgcggttgg cgcgcgtgta
     4561 ggcgcgggcc agcgcgaagt ggatgttcgg cgccagcggc gcgagactcc gggccgcctc
     4621 cagctctttg atggaacggt cgttctgccc cgcgtcgaag agcaggcgcc cgaggatgta
     4681 gtggccgacc gcgttgcggg gcgagagctt cagcgc
//
LOCUS       DBNGOH010000379         2136 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_240043, whole genome shotgun sequence.
ACCESSION   DBNGOH010000379 DBNGOH010000000
VERSION     DBNGOH010000379.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2136)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2136)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2136
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_240043"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(196..1209)
                     /locus_tag="AC159P_07555"
     CDS             complement(196..1209)
                     /locus_tag="AC159P_07555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HIR3695348.1"
                     /translation="MNSSQQSLRFFIVVCLVACALAAACGASGAKKAPLPSQVRKQAK
                     DAPTPRPEERAAYGPPARLGQFDDDAINESSGVAASRANAGLYWTHNDSGDGPYLYAF
                     DRRGAKRGVWRVAGAKADDWEDMAAGPGPERGRTYLYIGDIGDNDYARSSLIVYRVAE
                     PTVTEEDADSSKKRPRETAPAEAILLKYPDGSHDAETLLVHPQTGDLYIVLKSLDPAA
                     GVYKLAAPFDSTKMHTLARVGEVRALGAPFGGYFTGGAVSPDGTRLVLCDYLHAYEMT
                     LPAGAGGFDAVWKQPLLVVALGERRQGEAVTYGPDGDTILATNEGRRGAVVEVKRQVG
                     SRR"
     gene            complement(1239..1931)
                     /locus_tag="AC159P_07560"
     CDS             complement(1239..1931)
                     /locus_tag="AC159P_07560"
                     /inference="COORDINATES: protein motif:HMM:NF033545.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HIR3695349.1"
                     /translation="MKPHLKQTWCIGQITGEYLARMEDVLHLYSLPHDEQRPVVCFDE
                     MPVQLLGEVVAPLPVKAGKPARFDYEYERGGTACLLVAFEPLTGRRLIETSKQRTKAD
                     YCRFMQRVCEMFPRAEKVVLVQDNLNTHNASSFYENLLPEEAFALAQRFEMHYTPKKG
                     SWLNVAELELSALSRICLARRIASIAELDREVQALVKERNELKVKVEWQFSVTQAREK
                     LSRHYENAKSMN"
     gene            complement(1928..2113)
                     /locus_tag="AC159P_07565"
                     /pseudo
     CDS             complement(1928..2113)
                     /locus_tag="AC159P_07565"
                     /inference="COORDINATES: protein motif:HMM:NF024954.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT          333 a          707 c          710 g          386 t
ORIGIN      
        1 ggtagcaaaa ggcggcgacg gcgggaagcc tgtagaagag tgggcagtag gcggtaggca
       61 gtaggcagca ggcagcaggc agtcaaaaca gaagggcgaa gacgcttgag aggtaaagtc
      121 ctgagcctct ccgcgcttca agcaacaaag ccagaggctt gagacgttag ctctcaagcc
      181 tcctgcttta actgcctacc gcctactgcc tacttgcctt ttgacttcga cgacggcgcc
      241 gcggcgtcct tcgttggtcg ctagaatcgt gtcgccgtcg gggccgtaag tgacggcctc
      301 gccctgtctg cgctcgccga gggcgacgac gagcagcggc tgtttccaga cggcgtcgaa
      361 gccgccagcg cccgcgggca gcgtcatctc gtaggcgtgg aggtagtcgc agaggacgag
      421 gcgcgtgccg tcgggcgaga cggcgccgcc cgtgaagtag ccgccgaagg gagcgccgag
      481 cgctcgcacc tcgccgacgc gcgcgagcgt gtgcatcttc gtcgaatcga acggcgcggc
      541 cagcttgtag acgcccgccg cggggtcgag cgacttcaag acgatgtaaa ggtcgccggt
      601 ctgcggatgc acgagcaagg tctccgcgtc gtgcgagccg tcagggtact tcaaaaggat
      661 tgcctctgcg ggcgcggtct cgcgtgggcg cttcttgctc gaatcggcgt cctcttcggt
      721 gacggtcggc tccgccacgc ggtagacaat gagcgaggag cgcgcgtagt cgttgtcgcc
      781 gatgtcgccg atgtagaggt acgtgcgccc gcgctccggc cccggccccg ccgccatgtc
      841 ttcccagtcg tccgccttcg cgcccgcgac gcgccacacg ccgcgcttcg cgccccgccg
      901 gtcgaaggcg tagaggtagg ggccgtcgcc cgagtcgttg tgcgtccagt agagaccggc
      961 gttcgcgcgc gaggcggcga cgccgctcga ctcgttgatg gcgtcgtcgt cgaactggcc
     1021 gagccgcgcg ggcgggccgt aggccgcgcg ctcttcgggc cgcggcgtcg gcgcgtcctt
     1081 cgcctgcttc ctgacctgcg aagggagcgg cgccttcttc gcgcccgacg cgccgcacgc
     1141 cgccgcgagc gcgcacgcga cgaggcagac gacgatgaag aaccgtaggc tttgctgaga
     1201 gctattcatt gcgctttgca tcctagtgct ctgtaatttt agttcataga cttcgcattc
     1261 tcgtagtggc ggctcagctt ctcccgcgcc tgcgtgaccg agaactgcca ctcgactttg
     1321 accttgagct cgttgcgctc tttgacaagc gcctgcacct cgcgatcaag ctccgcgatg
     1381 gaggcgatgc gccgcgcgag gcagatgcgc gacagagccg acaactccaa ctccgccacg
     1441 ttgagccacg accccttctt cggcgtgtag tgcatctcga agcgttgcgc cagcgcgaag
     1501 gcttcctcgg gcagcaggtt ctcgtagaag gagctggcgt tgtgcgtatt caggttgtcc
     1561 tgcaccagca ccaccttctc cgcccgcggg aacatctcgc agacgcgctg catgaagcgg
     1621 cagtagtcgg ctttcgtccg ctgcttgctc gtctctataa gccgccgccc cgtcaaaggc
     1681 tcgaaggcga ccagcaggca ggccgtcccc ccgcgctcgt actcgtagtc gaagcgcgcg
     1741 ggctttccgg ccttcaccgg caggggcgcg accacctcgc cgagcagttg caccggcatc
     1801 tcgtcgaagc agacgacggg gcgctgctcg tcatgcggca ggctgtagag gtgcagcacg
     1861 tcttccatcc gcgccaggta ctcgcccgtg atctgcccga tgcaccacgt ctgcttgagg
     1921 tgcggcttca gttcgttttt ttcagcacct ctttgaccgt gttgtgtgag atcgcttcga
     1981 ccaggccgag ttcgaccgcg cggtcagcca gcagccgcaa gctccagcgg gcgtgcccct
     2041 ccggcgcttc ggagcaggcg tgcgccgtaa tcttggcccg cgccacgccg tcgatgcggg
     2101 cgggcttgcc ggagcgcggc ttatcctaga gcgcag
//
LOCUS       DBNGOH010000380         5152 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3172504, whole genome shotgun sequence.
ACCESSION   DBNGOH010000380 DBNGOH010000000
VERSION     DBNGOH010000380.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5152)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5152)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5152
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3172504"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(187..564)
                     /locus_tag="AC159P_07570"
     CDS             complement(187..564)
                     /locus_tag="AC159P_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012575210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR3695350.1"
                     /translation="MKIRFNRLNHVQVCVPPGAEDEARGFYGRLLGLQEIEKPEVLRA
                     NGGLWFEIAGVQLHIGVEPLQPPSKRHPAFEVEDLEAVRAYLGASGVRLKEEPSIPGA
                     RRLSFFDPFGNRIELLERTNADG"
     gene            complement(714..812)
                     /gene="ffs"
                     /locus_tag="AC159P_07575"
     ncRNA           complement(714..812)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="AC159P_07575"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(935..1459)
                     /locus_tag="AC159P_07580"
     CDS             complement(935..1459)
                     /locus_tag="AC159P_07580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695351.1"
                     /translation="MRKTITFGVALLLLAWVIAPALAEGAAGQRRSQRRQLALCPDPT
                     VRCRTSYEFKPHQLPFALPENAVLWETEQFYAVVLKSVRDGDAAQDCSVFIPEDERLA
                     AQQLFPRHKVFASRCYDPEELYYTNVADGQQFMAVYAGRTRAEAERMLAKVKATGKYK
                     DANLRRMRTGFNGT"
     gene            complement(1554..1643)
                     /locus_tag="AC159P_07585"
     tRNA            complement(1554..1643)
                     /locus_tag="AC159P_07585"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1607..1609),aa:Ser,seq:gga)
     gene            complement(2030..3043)
                     /locus_tag="AC159P_07590"
     CDS             complement(2030..3043)
                     /locus_tag="AC159P_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012403879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HIR3695352.1"
                     /translation="MTSSVTPERIMQLGLGFWGSKTLLSAVELGLFTELAAGPLDAPT
                     LRDRLGLHPRAARDFLDALVSLRVLDRDAEGRYSNTSEADTFLDRAKPSYLGGMLEMA
                     NARLYPFWGSLTEALQTGRPQNEVKSGGNFFEALYAEPERLEGFLGAMTGLSLGAARA
                     IAQQFPWRRYKTFMDIGGAQGGLPVAVALAHPHLVGGNFDLAGVGPVFEKYVARHGLS
                     DRLRFHPGDFFEDPMPSADVLVMGHILHDWDMEEKRVLIAKAYDALPEGGSLIVYEAL
                     IDDERRENTLGLLMSLNMLIETPGGFDYTGADCQAWMKEAGFRETRVEHLVGPDSMVV
                     GVK"
     gene            3415..>5152
                     /locus_tag="AC159P_07595"
     CDS             3415..>5152
                     /locus_tag="AC159P_07595"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018475323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 protein"
                     /protein_id="HIR3695353.1"
                     /translation="MFGRLALGVLLLAVVAPCVAAQGDASQTQVRYLSGTGNDDGVEW
                     DFFCTGGRKAGTWTKIPVPSHWEQHGFGGYNFGRPHDEKANPLAREQGKYRRRFDVPA
                     AWRGRVVRLVFDGVMTDAEVFVNGKPAGPLHQGSFYRFEYDITPLLKFGGPNLLEVTV
                     SKVSANESVNRAERFRADYWVFGGIFRPVYLEALPASFIDWTGIDARADGSFNVDVHL
                     GGRTSQGRLTAQLIDAHGAAVGKPFSAEVAPNQETVRLETKIAAPKLWTAETPNLYRV
                     RLTLSPQSHPQHVVTERFGFRTFEVRPGDGLYLNGQRIILKGTNRHSFWPESGRTLSR
                     EISYDDVRLLKEMNNNAVRMSHYPPDEHFLEACDELGLYVLDELGGWQRSYDTETGRR
                     LIGEMIRRDVNHPSILFWDNGNEGGWNKENDDEFAKWDIQKRPVLHPWELFRGVNTDH
                     YENYESHARLASGPDIYMPTEFLHALYDGGAGAGLWDYWEVLRRSPVGAGGFIWALLD
                     ECVARTDMGGRLDCSGNQGADGIVGPHREKEGSFYTVREIWSPVQVVGADELPEVFRG
                     VLAVENRYDFTNL"
BASE COUNT          895 a         1693 c         1694 g          870 t
ORIGIN      
        1 gcgcgggccc gagaagaggt aggcgtggtg caggcgctcg tgctcgatgg cgttgcggag
       61 cgtgcgcgtg atgtgctcct gccccgtcac ctcctcgaac gcctgcgggc gccacttgcg
      121 tgcgatgacc tgataagaca taaggcggca gtcagctatc agcagtcagc tatcggcagt
      181 cagttatcaa ccgtcggcgt tcgttctttc caacagctcg atgcggttgc cgaaggggtc
      241 gaagaaggag aggcggcggg cgccggggat cgaaggctct tccttcagcc gcacgccgct
      301 cgcgccgagg tacgccctca cggcttcgag gtcttccacc tcgaaggcgg ggtggcgctt
      361 cgagggcggc tgcaacggct cgacgccgat gtgaagctgg acgccggcga tctcgaacca
      421 cagcccgccg ttcgcgcgga gcacttcggg cttctcgatc tcttgcagcc cgagcagccg
      481 cccgtagaac ccgcgcgcct cgtcttccgc gccgggcggg acgcagacct gcacgtgatt
      541 cagcctgttg aatcgaatct tcatggcgac cgcggtgtct cgaaaaaaga aaagagttgc
      601 cgcgagcgac ctccgcgggt gcgcgttggc ggcgtcgctg gattgtgctc caacagcgcc
      661 gacttcggca acccggaagc ggctacgaac ggcaactctc ttccgatgtg gccagactcc
      721 ggaccgaccc gcaacacacg ccgccgccgc taccgttgct ccgttcccgg cctggcgggg
      781 ttcgcggggc aagcgttgtg cggagcccga agtctgataa attttcaagg agctgttggc
      841 gattagccgt cagctcttag cttttagact ttaacttttt ccgaccgcat cggcaactcg
      901 ccagcgccgg tctcgctcga cgaagggcgc tctctcaagt gccgttgaag ccggttcgca
      961 tgcgccgcag gttggcgtcc ttgtacttgc cggtggcctt caccttcgcc agcatccgct
     1021 cggcctcggc gcgcgtgcgg cccgcgtaga cggccatgaa ctgctgacca tcggcgacgt
     1081 tcgtgtagta cagctcttcg gggtcgtagc agcgcgacgc gaagaccttg tggcgcggaa
     1141 aaagttgctg cgccgcgagg cgctcgtcct cggggatgaa gacgctgcaa tcctgcgccg
     1201 cgtcgccgtc gcgcacgctc ttcagcacga cggcgtagaa ctgctcggtc tcccacagca
     1261 ccgcgttctc cggcagggcg aacggcaact ggtgcggctt gaactcgtag ctcgtccggc
     1321 agcgcacggt cgggtcgggg cagagcgcca actgccgccg ctgactcctc cgctgccccg
     1381 ccgcgccctc cgcgagcgcc ggcgcgataa cccacgcgag caacaacaac gcgacgccga
     1441 acgtgatcgt tttacgcata atcgaatgag gtcagtggcc ggaggtcggt ggtcagtgaa
     1501 agcaagaagt ttttactgac ctctgacctc cggccaccgg ccattgcgtt ttgtggcgga
     1561 gagggtggga ttcgaaccca cggtacggtt ccccgtacac agcatttcca gtgctgccag
     1621 ttcaaccact cctgcacctc tccgaaaagc tgttagcggt tagcttttag ctgtcagccg
     1681 ttgaagaaaa gccaacagcc ggaagccaat cgctaacagc ttcgatacgt cagaattgcc
     1741 aagaattcaa acagcggcga accggcaaag cataatgcat cgcggccatt cctgtcacgc
     1801 catgacgccg accgcgagcc gtgtgtgaac gcggcgcgtc agggggtggt gatagcgggg
     1861 cgcggggcgt tggccccggc ggcctggcgc gagttgaagg tcgcctgata gctcgtcgtg
     1921 accgtcgcct tgaggacgaa ggcgacggtg ctccggcccg cggcgaatct cctcgacgca
     1981 gcaggtgtta acgaccccgc ggcggggcgt cgcgccgcgc gggccgaagc tacttgactc
     2041 cgacgaccat cgaatcaggc ccgacgaggt gctcgacgcg cgtctcccga aagcccgcct
     2101 ccttcatcca cgcctgacag tccgcgcccg tgtaatcgaa cccgcccggc gtctcgatca
     2161 gcatgttcag gctcatcagc agccccagcg tgttctcgcg ccgttcgtcg tcgatgagcg
     2221 cctcgtagac gatgagcgag ccgccctccg ggagcgcgtc gtaggctttc gcgatcagca
     2281 cgcgcttctc ttccatgtcc cagtcgtgca ggatgtgccc catcacgagc acgtccgccg
     2341 aaggcatcgg gtcttcgaag aagtcgccgg ggtggaagcg cagccgatcc gagaggccgt
     2401 ggcgcgcgac gtacttctcg aagacaggcc ccacgcccgc gaggtcgaag ttcccgccca
     2461 cgaggtgcgg gtgggcgagc gcgaccgcga ccggcagccc gccctgcgcg ccgccgatgt
     2521 ccatgaaggt cttgtaccgg cgccacggga actgctgcgc gatggcgcgg gccgcgccga
     2581 ggctgaggcc ggtcatcgcg ccgaggaacc cttcgagccg ctccggctcg gcgtacaggg
     2641 cctcgaagaa gttgccgccg ctcttgacct cgttctgcgg gcgacccgtt tgcagcgctt
     2701 cggtcagcga accccagaag gggtagaggc gcgcgttcgc catctccagc atgccgccga
     2761 ggtaggaggg cttggcccgg tcgaggaacg tgtccgcctc ggacgtgttc gagtagcggc
     2821 cttcggcgtc gcggtcgagc acgcgcagcg agacgagcgc gtcgaggaag tcgcgcgcgg
     2881 cgcgcgggtg aaggccgagg cggtcgcgga gcgtcggggc gtcgagcggg cctgcggcca
     2941 gttcggtgaa gaggccgagt tcgaccgcac tcagcaaggt cttcgaaccc cagaagccga
     3001 ggccgagctg catgatccgt tcgggggtga ctgaagaagt catgtcgcgt ctccttaata
     3061 ttcatggcgt ccgcagagcg gtcgctgttc gtcgaacggc ctcgtctcac caacttcgac
     3121 acaaggccga ggagagacgg ctcccgcgcg gcgccgatgg cccccctcca tttgcttaac
     3181 gcgaaatcgg cggatgtttc ggctcacgtc cgccgactcc ttccagcgct tcgcggcgca
     3241 accgacgcag gccgccagac ctgcctttcg cgatgccacg gaaataaaag cggaagtctt
     3301 taccggcggg ctgtggtacg tttaccacgc gtcgggcgcg gcgacgcgca cgcgtcccta
     3361 tgaagagttt cttccccggc gtgagtctag cacggtgtga cgaggtgacg gcttatgttc
     3421 ggacggttgg cgctcggagt cttactcctg gcggttgtcg cgccgtgcgt cgccgcacag
     3481 ggcgacgcct cccaaacgca agtccggtat ctttccggca cgggcaatga cgacggcgtc
     3541 gagtgggact tcttctgcac gggcggacgc aaggcgggca cgtggacgaa gattccggtg
     3601 ccttcgcatt gggagcagca cggcttcggc ggctacaact tcgggcgacc ccacgacgag
     3661 aaggcgaacc cgctcgcgcg cgagcagggg aagtatcgac ggcggttcga cgttcccgcc
     3721 gcatggcgag gcagggtcgt ccgcctcgtc ttcgacggcg tgatgaccga cgccgaagtc
     3781 ttcgtcaacg gcaagcctgc cggccccctc catcaaggct ccttctaccg cttcgagtac
     3841 gacatcaccc cgctcctgaa attcggcggg ccgaacctgc tggaagtcac cgtcagcaag
     3901 gtctcggcga acgagagcgt caaccgcgcc gagcgcttcc gcgcggacta ctgggtcttc
     3961 ggcggcatct tccgcccggt ctacctcgaa gccctgcccg cctccttcat cgactggacg
     4021 ggcatcgacg cgcgcgccga cggcagcttc aacgtggacg tgcatctcgg cgggcgcacg
     4081 tcgcagggcc ggttgacggc gcagcttatc gacgcgcacg gcgccgcggt cggcaagccg
     4141 ttcagcgccg aagtggcacc gaaccaggaa acggtcagac tcgaaacgaa gattgccgcg
     4201 ccgaagctct ggacggcgga gacccccaac ctctaccgcg tgcgtctcac actgtcgccg
     4261 caatctcatc cgcaacacgt cgtcaccgag cgcttcggct tccgcacgtt cgaggtgagg
     4321 ccaggcgacg ggctgtacct gaacggccag cgcatcatcc tgaaaggcac gaaccgccac
     4381 tcgttctggc ccgagtcggg tcgcacgttg agccgggaga tcagctacga cgacgtgcgg
     4441 ctgctcaagg agatgaacaa caacgcggtg cggatgtcgc actaccctcc ggacgagcac
     4501 ttcctcgaag cctgcgacga gctgggtctc tacgtcctgg acgagctggg cggctggcag
     4561 cgcagctacg acaccgagac gggccgacga ctcatcggcg agatgattcg ccgcgacgtg
     4621 aatcatccct ccatcctctt ctgggacaac ggcaacgagg gcggctggaa caaggagaac
     4681 gacgacgagt tcgcgaagtg ggatattcag aaacgccccg tcctccaccc gtgggagctg
     4741 ttccgcggcg tcaacacgga tcactacgag aactacgaga gccacgcgcg gctcgcaagc
     4801 gggccggaca tctacatgcc gaccgagttc ctgcacgcgc tctacgacgg cggcgcgggc
     4861 gcggggctgt gggactactg ggaggtcttg cgcaggagcc ctgtcggcgc gggcggcttc
     4921 atctgggcgc tcttggacga gtgcgtcgcg cgcacggaca tgggcgggcg gctcgactgc
     4981 tccggcaatc agggcgcgga cggcatcgtc ggcccgcacc gcgagaagga ggggagcttc
     5041 tacacggtgc gcgaaatctg gtcgcccgtg caggtcgtag gcgcggacga actgcccgaa
     5101 gtctttcgcg gcgtccttgc ggtcgagaac cgctacgact tcacgaatct ca
//
LOCUS       DBNGOH010000381         1962 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_5005737, whole genome shotgun sequence.
ACCESSION   DBNGOH010000381 DBNGOH010000000
VERSION     DBNGOH010000381.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1962)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1962)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1962
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_5005737"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(92..1306)
                     /locus_tag="AC159P_07600"
     CDS             complement(92..1306)
                     /locus_tag="AC159P_07600"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013557461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HIR3695354.1"
                     /translation="MRKTFTLIAALTLLTVALVTANVHSSTAAEKSAEKKEEKLLRKA
                     AGKIAGSYIVVFNEWATGPTGEFSQAAEMADFMKTVYGGRVKHVYKHALNGFAAEMSE
                     AAALALSTDPRVAYVEEDAEVSIDATQNNPPWGLDRIDQRDRPLSATYTYNWTGSGVR
                     VYIIDTGIRTSHTQFGGRASAVFDVNGGNGQDCNGHGTHVAGTVGGSTYGVAKSALLR
                     GVKVFQCGNTTSTANIIAGVNWVTANRILPAVANMSVGGPASSSMDTAVQNLINSGVT
                     VAVAAGNSNGANACNFSPARLAAAITVGSTTSTDTRSSFSNIGTCLDIFAPGSGIQSA
                     WWTSTTATATISGTSMASPHVAGVAALYKQANPSASPATIRNAIVNNSTTNRISNVGT
                     GSPNRLLYSLFF"
     gene            1684..>1962
                     /locus_tag="AC159P_07605"
     CDS             1684..>1962
                     /locus_tag="AC159P_07605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695355.1"
                     /translation="MHGRGKRAFALAVLALSMLSSAWPVAAQDGDLMSSSGVFVDPPS
                     ARRHQQRRERARASRKFEPRRKPAATTVATATPQPTPTPQPTSQPTPRP"
BASE COUNT          298 a          621 c          701 g          342 t
ORIGIN      
        1 cgaacaaaga ggcttgggac tttacctccc aagtctcttc gcccttctct gtttttactg
       61 cctgctgcct gctgcccgct gccgactcac gtcagaagaa cagcgagtag agcagccggt
      121 tgggcgagcc tgtgccgacg ttggagatgc ggttggtcgt cgagttgttg acgatggcgt
      181 tgcggatcgt ggcgggcgag gccgaggggt tggcctgctt gtagagcgcg gccacaccgg
      241 cgacgtgggg cgaggccatc gaggtgccgc tgatggtggc ggtcgccgtg gtcgatgtcc
      301 accacgcgga ctggatgccc gaccccggcg cgaagatgtc gaggcaggtg ccgatgttgg
      361 agaacgacga gcgcgtgtcc gtggaggtgg tcgagccgac ggtaatcgcg gcggcgagcc
      421 gcgcgggcga gaagttgcac gcgttcgcgc cgttgctgtt gcccgcggcg accgccaccg
      481 tgacgcccga gttgatgagg ttctgcaccg ccgtgtccat cgacgaagag gccgggccgc
      541 cgacgctcat gttcgccacc gccggcagga tgcggttggc cgtcacccag ttgacgcccg
      601 cgatgatgtt cgcggtcgag gtcgtgttgc cgcactggaa gaccttgacg ccgcgcagca
      661 gcgcgctctt ggcgacgccg taagtgctgc cccccacggt gcccgcgacg tgcgtgccgt
      721 ggccgttgca gtcctgcccg ttgccgccgt tcacgtcgaa gaccgccgag gcgcgcccgc
      781 cgaactgcgt gtggctggtg cgaatgccgg tgtcgatgat gtagacgcgg acgcccgacc
      841 ccgtccagtt gtaggtgtag gtggcgctca gtgggcggtc gcgctggtcg atgcggtcga
      901 ggccccacgg cgggttgttc tgcgtggcgt cgatcgacac ctccgcgtcc tcttcgacgt
      961 aggcgacgcg cgggtcggtg ctgagcgcga gggccgccgc ctccgacatc tcagccgcga
     1021 agccgttgag cgcgtgcttg tagacgtgct tgacgcgacc gccgtagacc gtcttcatga
     1081 agtcggccat ctcggcggcc tgcgagaact cgccggtcgg ccccgtcgcc cactcgttga
     1141 agacgacgat gtagctgccc gcgatcttgc ccgcggcttt gcggagcagc ttctcctctt
     1201 tcttctccgc ggacttttcg gcggcggtgc tgctgtgtac gttggccgtc acgagcgcga
     1261 cggtgagaag cgtgagcgca gcgatgagcg tgaaagtttt tctcatgttc cctccaaagt
     1321 aggttgcggt caaaggtgag cgtatcggga aacgaaggcc cgccgtgatc ccttcgaggg
     1381 gcagagaaag ggaagcggtc ggagccgggc cgagcgataa aactgtcatc tccagtggtg
     1441 agtgccgagc actatattca tcttcggcga aacgcgcaag ggctttccgc ggggagaaag
     1501 tgaggttttc ttcacggcga aagtcgcgcc gccgccgcgc ccggaaacgg cggggcggtc
     1561 aggccgcgcc cgcggcgcac cagtggtgaa aatcggcacg gcggtttaaa ctcgcagccg
     1621 tacgggggcg ctcgaaccgc cccgccgcgc gtgccgtata tcgatcagga ggaggtgttg
     1681 acgttgcacg gaagaggaaa gcgtgcgttc gccctggcgg tgctggccct gtctatgttg
     1741 tcgtccgcat ggccggtcgc ggcgcaggac ggcgacctga tgagcagctc cggcgtcttc
     1801 gtcgatccgc caagcgcgcg caggcaccag cagcggcgcg agcgcgcgcg ggccagccgc
     1861 aagttcgagc cgcgccgcaa gcccgcggcg acgaccgtgg ccaccgccac gccgcagccg
     1921 acgccgacgc cgcagcctac atcgcagcct acgccgcggc cc
//
LOCUS       DBNGOH010000382         3182 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4639333, whole genome shotgun sequence.
ACCESSION   DBNGOH010000382 DBNGOH010000000
VERSION     DBNGOH010000382.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3182)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3182)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3182
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4639333"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1159)
                     /locus_tag="AC159P_07610"
     CDS             complement(<1..1159)
                     /locus_tag="AC159P_07610"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0140905 - haloperoxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vanadium-dependent haloperoxidase"
                     /protein_id="HIR3695356.1"
                     /translation="MATLAMLLAVSAGVRADAVTDWNEIAVQRMNAAVPAVPPPIAFV
                     NLAIVQVAVYDAVQAIEGRFEPYHVEIPGASGSPEAAAAKAAHDVLVHLFPAQAPLLA
                     MTYQQYLSDHGLAADDPGVTVGATAAAGIIALRANDGRNPFPAPTPFVGGTGPGEWRP
                     TDSLIGPPFAPPPFAPMAAPWTANVTPFTLKSGDQWRAAPPPRLTSHQYTRDYNEVKA
                     MGARFDSARTPEQTDFAIFWASNYAALWNRAFRDIAAAHVHDIGDSARLFALANMAMA
                     DAAITAWDSKITYNFWRPITAIRLGDDDTNPQTVGDPDWQPLINTPNYPDHTSGANAT
                     TGAATRALALYFGTEDFTFTVRTTNAGTIQQTRTYTSFSDASEDVVTARVYE"
     gene            complement(2073..2267)
                     /locus_tag="AC159P_07615"
     CDS             complement(2073..2267)
                     /locus_tag="AC159P_07615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695357.1"
                     /translation="MALLVRETETAVVGLTSGRRRIDLDVQWALIRDSGEFEAAGVFQ
                     TFKAHSHEALAGVRGRAFNL"
     gene            2550..>3182
                     /locus_tag="AC159P_07620"
     CDS             2550..>3182
                     /locus_tag="AC159P_07620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695358.1"
                     /translation="MFFGVLATAVAMLLGIPLSRTVQSAGGGQETKQRAPGGVVRAPA
                     APGQTGGRRTKPGQDESGNKSKRYPNVDVRVTEPGALAETPGANAAAVAKQAQARKQA
                     IEQGLSRLRASSRGVEARLSPLTGAVEVLRSKTGALTAAAPGRPGEEIVRDFIRDNGA
                     LYGLTRGDIDNLRFLGESVSQGSGMRMVRVEQVVNGLPVFQSETRFILDAD"
BASE COUNT          553 a          955 c         1089 g          585 t
ORIGIN      
        1 cctcgtagac gcgggcggtg accacgtcct cggaagcgtc cgagaaactc gtgtacgtgc
       61 gcgtctgctg gatggtcccc gcgttggtcg ttctcaccgt aaaggtgaaa tcctcggtcc
      121 cgaagtagag cgccagcgcg cgggtcgcgg cacccgtggt ggcattcgca ccggaggtat
      181 ggtcggggta gttcggggtg ttgatcagcg gctgccagtc ggggtctccg accgtctgcg
      241 ggttcgtgtc gtcgtcaccc agcctgatgg ccgtgatggg ccgccagaag ttgtaggtga
      301 tcttgctgtc ccaggcggtg atcgccgcgt ctgccatcgc catgttggcc agtgcgaaga
      361 gccgtgcgct gtccccgatg tcatggacgt gcgccgccgc gatgtcccgg aaagcgcggt
      421 tccagagcgc ggcgtagttg gaggcccaga agatggcgaa gtcggtctgc tcgggcgtcc
      481 gcgcgctgtc gaagcgggcg cccatggctt tcacctcgtt gtaatcgcgg gtgtactggt
      541 ggctggttaa acgcggcggc ggcgcggcgc gccactggtc tccgctcttc agtgtgaagg
      601 gggtgacgtt ggccgtccac ggcgcggcca tcggggcgaa gggcggcggc gcgaagggcg
      661 ggccgatgag agagtccgtc gggcgccatt cgccggggcc ggtgccgccg acgaagggcg
      721 tcggcgcggg gaacgggttg cgcccgtcgt tggcccgcag ggcgatgatg ccggcggcgg
      781 cggtcgcccc gaccgtcacg ccggggtcat ccgccgcgag gccgtggtcg ctaaggtatt
      841 gttggtaggt catggcgagg agcggggctt gcgcggggaa gaggtggact aacacgtcgt
      901 gcgcggcctt cgcggcggcg gcctccggcg agccggaggc tcccggaatc tccacgtggt
      961 acggctcgaa ccgcccctcg atggcctgga cggcgtcgta caccgccacc tggacgatgg
     1021 ccaggttgac gaaggcgatt ggcgggggga cagccgggac agccgcgttc atcctctgca
     1081 cggcgatctc attccagtcg gttacggcgt cggccctgac gccggcagag actgcaagca
     1141 gcatggcaag ggtagccatg gctctgcatg cttgaaatct aactctcatg tctcctcctt
     1201 ctgttactgc gagcagttgg tctgaagtcc gtttatcggg tcgggaagat gtcactccac
     1261 ttcctgaagt gacgttaggt tctcctttgg actgtggtcg gcaacttgcg gtggccggca
     1321 gcaggctcct ttcggaaggg cgaggagtgg cgggtcaatc ccaaatcaga cttgccgtcc
     1381 caccggttga catgccgggt gatgcccttt gggggcggcg tcctcaacta tctatgcgaa
     1441 tctggggcga ggcatttcac ggggtcgccg acggacggcg gatgaactga acccgttatc
     1501 gaaacgggtc actaccgcgc ggggtggttg cttgctacct ctgcaacgag aacaagcaaa
     1561 agctggagag cgagtgcgcc gattataaat gcggcgcgga gacaggcccg caaagtatga
     1621 agaggcggga aagctgcgga ttagtaaagg ggcctgtctc gggcggtggg atactatgcc
     1681 cggttccggg gcgcgtcaag accgattatg cgccggcggc atggccccgg caaaaaagaa
     1741 agatttacaa atttccgtgt aagccggcgg gcttgcggct atcactgttc atggtcggcc
     1801 atttatttcc gacaggcagc cccggcgtgg taaagaaccc aacggcgtgg ttgcggtagg
     1861 catgtccggg ttggaaatca cggtgtcggc gattcgattc cttccccgcc gtcaaccaaa
     1921 atcgaacggt tacgggcggc cccaaagccc tgtctagaat ccctccgggc agtctcgccg
     1981 gacgttgtct attttcatgg aaatattgtt tgacagccta tgtaacgtga gtcaaaatcc
     2041 ccccactcac cagcgtcatt cgtaggtccg cgtcagaggt tgaaagctcg gccacgaaca
     2101 cccgcaagtg cttcatggct gtgtgccttg aatgtctgga agacgccggc ggcctcaaac
     2161 tctccggagt cacgaatcag tgcccactga acgtcgaggt cgatacggcg ccgccccgaa
     2221 gtcagcccga caaccgccgt ctcggtttcc ctcaccagca gtgccaatag ttacgcgagc
     2281 aaggtgaagc gcgcgggcgc ccaccggctc aagccctact cacgcacgga aaaggtttac
     2341 gagatttcct cgcggatgac ttttacggtc aagtagagct gtcgcggggc gtctcttcct
     2401 gcggccctat accctcgcat tgcaatcccg atgacattag caaccaccgt gaggtggtat
     2461 tccctctcgc cggtagtctg tcttgtcggc tgaccttaac caaggagaac ctgatgtcgt
     2521 tttactcccc gtccgtttcg cgcaagaaat tgtttttcgg agtgctggcg accgccgtcg
     2581 ccatgttgtt aggcatcccc ctttcccgca cggttcagag tgccggcggc ggccaggaga
     2641 cgaagcagcg cgcgccgggc ggcgtcgtcc gcgcgcccgc ggctcccggc cagacgggag
     2701 gccggaggac gaagcccgga caggacgagt ccggaaacaa atcgaagcgt taccccaacg
     2761 tggacgtgag ggtgacggag ccgggcgcgc tggccgagac gcccggagcg aatgccgcgg
     2821 cggtcgccaa acaggcgcag gcacggaagc aggcgataga gcagggcctc tcgcgcctca
     2881 gagcctcctc gcgcggcgtc gaggccaggc tctcgccgct caccggcgcg gtcgaggtct
     2941 tgcgcagcaa gacgggcgcg ctgaccgcgg cggcgccggg tcgccccggc gaagagatcg
     3001 tgcgcgactt catccgcgat aacggcgccc tctacgggct gacgcgcggc gacatcgaca
     3061 acctgcgctt cctcggcgaa agcgtcagtc aggggagcgg tatgcggatg gtgcgcgtcg
     3121 agcaggtggt caacggcctg cccgtcttcc agagcgagac gcgtttcatc ctggacgcgg
     3181 ac
//
LOCUS       DBNGOH010000383         3158 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_424674, whole genome shotgun sequence.
ACCESSION   DBNGOH010000383 DBNGOH010000000
VERSION     DBNGOH010000383.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3158)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3158)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3158
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_424674"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1159
                     /locus_tag="AC159P_07625"
     CDS             <1..1159
                     /locus_tag="AC159P_07625"
                     /inference="COORDINATES: protein motif:HMM:NF024668.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HIR3695359.1"
                     /translation="THQRPLEKCLADVDESDVYIGIFAWRYGFIPTHGNPRKLSITEL
                     EYRHALKDKKKHCLIFLLAEDAQWLPQFMDSQTGDGDGGKSIRRLRAELSERHGVFFF
                     SNPDQLARAISNALFPIQYTETPTPASPPATVAAPPPARARAAAPVAATAHEGPRASA
                     RKGYPKLWKPGDRLRVRFLDGTPKQRRFVERFVPIWSAYANIRFEFGDDPAAEIRVSF
                     EQEGSWAYVGTDALKVAAGEPTANFGWVTDRTRDTEIDYTIIHEFGHVLGLLHEHQNP
                     QAKIPWKKEAVYKTYEGWSRDAVNFNLFSKWDPKHFPYEKPFDRESIMAFPLPSDFTG
                     KDFEMGQNMSLSPGDKEFIHRLYPFDEDGGGDVTTAAPSKRKPSKRRAGSR"
     gene            1246..1473
                     /locus_tag="AC159P_07630"
     CDS             1246..1473
                     /locus_tag="AC159P_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015114636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HIR3695360.1"
                     /translation="MNYQDYITIEPNKRGGKPCVRGLRITVYEVLEYLASDMTEEEIL
                     DDFPDLTREDLKACIAFAADRERKLMTVPAG"
     gene            1470..1802
                     /locus_tag="AC159P_07635"
     CDS             1470..1802
                     /locus_tag="AC159P_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015215313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HIR3695361.1"
                     /translation="MKLLFDENLSHKLVGLLADLYPDSIHVRDVGLKAADDPVVWEYA
                     KRNGLMIVSKDSDMHQRSFVFGYPPKVVWVRLGNCSTSDVETLLRDNFAAVTTFYEDE
                     YASFLSLS"
     gene            1983..>3158
                     /locus_tag="AC159P_07640"
     CDS             1983..>3158
                     /locus_tag="AC159P_07640"
                     /inference="COORDINATES: protein motif:HMM:NF012618.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HIR3695362.1"
                     /translation="MKMKCDRRWAKGVCLVVLLFVAQGREARAQEEARVAARLLHTLT
                     EQGAPLWLSPDGRTLALRGEDVQLYDVATGTLKAKIPVRPELTGPKGVFFTPDGKALV
                     VHTDRVQVVDANDGKVLREFAEGSAPINFYRRIHTGREETSYDESSGGYSTAYHGPSD
                     KETLQELPALDPSDRVVSPNWKSILVRKEDGAAEVYDFETGKPRFTLAPKLGPGEQVG
                     LHFDALGEFSPDGKLILTSHASRTPRLWDAATGELVADLAPHTHFVYGARFSPDGRFI
                     VTTSFDGVVRTWDGATGKFLHALGSAQDPTYFAAWNPRSDTFVTKSRKWEVDIRDAAT
                     GKLVARLDRKAIKEKFDNNFTFAYSPDGRRLLTRARNVDTVLSVLKSTMLLSPPGKKS
                     "
BASE COUNT          579 a         1051 c         1025 g          503 t
ORIGIN      
        1 gacgcaccag cggcctctgg agaagtgcct cgcggacgtg gacgagtcgg acgtgtacat
       61 cggcatcttc gcgtggcgtt acggcttcat ccccacgcac ggcaacccca ggaagctctc
      121 catcacggaa ctcgaatacc gccacgcgtt gaaggacaag aagaagcatt gcctcatctt
      181 cctgctcgcg gaggacgcgc agtggttgcc gcagttcatg gactcacaga cgggcgatgg
      241 cgacggcggc aagagcatca ggcgcctgcg cgccgaactt tcggagcgcc acggggtctt
      301 cttcttcagc aaccccgacc agctcgccag ggccatcagc aacgccctct tcccgatcca
      361 gtacacggag acgccgacgc ccgcctcgcc gcccgcgacc gttgccgccc cgccgccggc
      421 ccgcgcccgc gcggcggcgc cggtcgccgc gaccgcgcac gaaggcccgc gcgcgtccgc
      481 gcgcaaaggc taccccaaac tgtggaagcc gggcgaccgc ctgcgcgtgc gcttcctcga
      541 cgggacgccg aagcagcggc gcttcgtcga gcggttcgtg cccatctggt cggcctacgc
      601 caacatccgt ttcgagttcg gcgacgaccc cgccgccgag atccgcgtct ccttcgaaca
      661 ggaggggagc tgggcctacg tcggcaccga cgcgctcaaa gtcgccgcgg gcgagccgac
      721 cgccaacttc ggctgggtca cggaccgcac gcgcgacacc gagatcgact acaccatcat
      781 ccacgagttc gggcacgtcc tcggcctgct ccacgagcac cagaacccgc aggccaaaat
      841 cccctggaag aaagaggcgg tctacaagac ttatgaaggc tggtcgcgcg acgcggtgaa
      901 cttcaacctc ttctccaagt gggacccgaa acacttcccc tacgagaagc ccttcgaccg
      961 cgaatccatc atggccttcc cgctcccttc ggacttcacc ggcaaggact tcgagatggg
     1021 ccagaacatg tcgctctcgc ccggcgacaa ggagttcatc catcgcctct accccttcga
     1081 cgaggacggc ggcggcgacg tgaccaccgc cgcgccctcg aagcgcaagc cctcgaagcg
     1141 gcgcgcgggt tcgcgctgaa gcggcgcggc gcggcggccc gcccccggcg acctgcggct
     1201 gcctgactct tgaccgcgcc aagcgtgcgg acataatctt cacgcatgaa ctatcaggac
     1261 tacatcacga tcgagccgaa taagcggggg gggaagccct gcgtccgggg cctgcggatt
     1321 acggtttacg aggtgctgga gtatctggct tcggacatga cggaggagga gatactcgac
     1381 gatttccccg acctcacccg tgaagacctg aaggcgtgca tagcctttgc cgccgaccgt
     1441 gagcgcaagc tgatgacggt gccggcggga tgaaattgct gttcgatgaa aacttgtcgc
     1501 acaagctggt gggtcttctt gcggatctct accctgattc gattcacgtg cgcgacgtgg
     1561 ggctgaaagc ggcggacgat cccgtcgtgt gggagtatgc gaagaggaac ggcttgatga
     1621 tcgtctcgaa agattcggac atgcatcagc ggagcttcgt gttcggctac cccccgaaag
     1681 tcgtgtgggt acggctcggc aactgttcga catcggacgt agagacgtta ttgagagata
     1741 acttcgccgc cgtcacaaca ttttatgaag acgaatacgc ttcgttcctg agcttgtcgt
     1801 aggacgcgct cgatcgaatc gtcacgcgcc cgcggcctga cgccgtctct ctctcccggc
     1861 gccgacctct cccatcttag tgccgaaaat ctcgtggtac ataatccgcc tccgggacgg
     1921 acaaccgggg ggttgcggtg tggctctaac ggcgttcgag gggtcgcgaa cgcgctcgga
     1981 ggatgaagat gaaatgcgac cggcggtggg caaagggcgt ctgtctcgtc gttttacttt
     2041 tcgtcgcgca gggccgtgag gcgcgggcgc aggaggaggc gcgtgtggcg gcccggctgc
     2101 tgcacacgct gacggagcag ggcgcgccgc tgtggctgag tcccgacggc aggacgctgg
     2161 ccttgagggg cgaggacgtg cagctctacg acgtggcgac cggcacgctc aaggcgaaga
     2221 ttcccgtcag gcccgagctg acggggccga agggcgtctt cttcacgccc gacggcaagg
     2281 cgctcgtcgt ccacacggat cgcgtgcagg tcgtggacgc gaacgacggc aaggtgctcc
     2341 gcgaattcgc cgaaggctcc gcgccgatca acttctacag gaggattcac acggggcgcg
     2401 aagagacttc gtacgacgag tcgtcgggcg gctattcgac cgcgtaccac gggccgagcg
     2461 acaaggagac cttgcaggag cttcccgcgc tcgacccgag cgacagggtc gtcagcccga
     2521 actggaagtc catcctcgtg cgcaaggagg acggcgcggc ggaggtctac gacttcgaga
     2581 cgggcaagcc gaggttcacg ctggcgccca agctcgggcc gggcgagcag gtcgggctgc
     2641 acttcgacgc gctcggcgag ttcagccccg acggcaagct catcctcacc tcgcacgcca
     2701 gccgcacgcc gcgtttgtgg gacgccgcga cgggcgagct ggtcgccgac ctcgcgccgc
     2761 acacgcactt cgtctacggc gcgcggttca gccccgacgg caggttcatc gtgacgacga
     2821 gcttcgacgg cgtcgtgcgg acgtgggacg gcgcgacggg taagttcctg cacgccctcg
     2881 gctcggcgca agacccgacc tacttcgccg cgtggaaccc gcgctcggac accttcgtca
     2941 ccaagtcgcg caagtgggag gtggacatcc gggacgcggc gacgggcaag ctcgtcgcgc
     3001 ggctcgaccg caaggccatc aaggagaagt tcgacaacaa cttcaccttc gcctacagcc
     3061 ccgacggcag acggctgctc acgcgggcgc ggaacgtgga caccgtcctc tcggtcttga
     3121 agtccaccat gttgctgtcg ccgccgggga agaagtcg
//
LOCUS       DBNGOH010000384         2963 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4823056, whole genome shotgun sequence.
ACCESSION   DBNGOH010000384 DBNGOH010000000
VERSION     DBNGOH010000384.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2963)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2963)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2963
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4823056"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(200..1114)
                     /locus_tag="AC159P_07645"
     CDS             complement(200..1114)
                     /locus_tag="AC159P_07645"
                     /inference="COORDINATES: protein motif:HMM:NF020123.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HIR3695363.1"
                     /translation="MKSLLLSLFILFAAAPLCAAQSQPVKLEGQVVCCEDCWARADRR
                     TVPFGTRADLEKAKECVAGGDPTLLAVLNEGGEATLYELEDGKYKRPGKDWLGFVGKR
                     VEVAGRVRAKGDKRFVKVDALKTLADAPAASEQLPNVVGNEAELVLKDLFGVEQRLGA
                     LRGRVVVLNFWATYCAPCRKEMPDLAAIQNQYAALGVQVVGASAETAEDRQKVLEFVR
                     ETKVNFPVWLGATVADMARFGLGPELPGTAIVGRDGKIVWLTRGVVNEADLKRQLDTL
                     LAQAAREANEQRAAAAKVRARDASSVPS"
     gene            complement(1301..1639)
                     /locus_tag="AC159P_07650"
     CDS             complement(1301..1639)
                     /locus_tag="AC159P_07650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695364.1"
                     /translation="MFGTLLPLCLLWGFIACVLLCLHHDEDSHDTVAVEVLDSGCGED
                     ACPVTDDTEFVTPGRPQSIPPAGEGWRACPDAAQTPEGVYASAPPVELEAAPTPALGP
                     PPERRCVLRI"
     gene            complement(1742..2863)
                     /locus_tag="AC159P_07655"
     CDS             complement(1742..2863)
                     /locus_tag="AC159P_07655"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013345.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HIR3695365.1"
                     /translation="MNLQTLKTPSLVLDAARVRRNAERIGSRVKQFGASLRPHVKTHK
                     CVEVARIQTAGHAGGITVSTLAEARAFAAHGFTDITYAVPIEPGKFEVAIELSKLCER
                     LYLLTDDVEIPPVLDVAARRAGVTLDLFMKVDCGYHRCGVEPHAPEALEIARRISDAP
                     HLRFAGILTHAGHSYHARSQEELLGLARHERDLMKDFAARLRDAGVEVPVVSVGSTPT
                     VTAVDHLDGVDEARPGNYIFFDAFQATLGSCAFEDCALSVVTAVVHRDRTRNKVVIDA
                     GAIALSKDRGAVEIDATCGYGRVLDLEGADTGLRVDALSQEHGVVVADDADVFDRLRV
                     GTRLRVLANHSCLTAAQHAHYNVCEGGRVVDRWEIHRGW"
BASE COUNT          484 a          953 c         1035 g          491 t
ORIGIN      
        1 tggggctgac cttgccggcg agcgggttgt attcgacgcc cgccgtgaag cgcgggtgga
       61 acctgtaggt cagggtgttg cgccagcgcg cgcgcggtat ctggttggcg atgacgcgga
      121 cgccgagcgt ccagcgcccc gattcgtgtg ggtcttgtgc tgccccgctc acggcgcggc
      181 cctcgccggg tcaaagcggt caggacggca cgctcgaagc gtcgcgcgcg cgaaccttcg
      241 ccgccgccgc gcgttgctcg ttcgcttcac gcgcggcctg cgcgagcagc gtgtcgagct
      301 gtcgcttcaa gtccgcttcg ttgacgacgc ctctcgtcaa ccagactatc ttgccgtcgc
      361 gcccgacgat ggccgtgccc ggaagttcag gcccgaggcc gaagcgcgcc atgtcggcga
      421 cggtcgcgcc gagccagacg gggaagttga ccttcgtctc gcggacgaat tcgagcacct
      481 tctgccggtc ttcagcggtt tcggcggaag cgccgacgac ctgcacgccg agcgccgcat
      541 attgattctg aatcgccgcg aggtcgggca tctccttgcg gcacggcgcg cagtaggtcg
      601 cccagaagtt gagcaccacg acgcgcccgc gcagcgcgcc caagcgttgc tcgacgccga
      661 agaggtcttt caagacaagc tcggcttcgt tgccgacgac gttcggcagt tgctccgaag
      721 cggcgggcgc gtcggcgagc gtcttcagcg cgtcaacctt gacaaaccgc ttgtcgccct
      781 tcgcgcgcac gcggcccgcg acctcgacgc gcttgccgac gaacccgagc cagtccttgc
      841 cgggccgctt gtacttcccg tcttcgagtt cgtaaagcgt cgcctcgccg ccttcgttca
      901 acacggcgag cagcgtcggg tcgccgcccg cgacgcattc cttcgccttc tccagatcgg
      961 cgcgcgtgcc gaagggcaca gtccgccggt cggcgcgcgc ccagcagtcc tcgcagcaca
     1021 cgacctgccc ttcgagtttg accggctgac tctgcgcggc gcacagaggc gcggcggcga
     1081 acagtatgaa cagagagagc agtaatgatt tcatcaaacg atcctcacat aacgctgact
     1141 tgttattcgg gacatgatgc cgacttgtta ttcggagagc gatgaaggct ccgcttcaag
     1201 acatgagagg cggtatcgcg tgctcgcaca tctactgagc cttgccccgt ctcgcgggta
     1261 aaaaggatta acttgaagtc ggattttctg taatacggaa tcagatgcga aggacgcagc
     1321 gcctttcggg cggcgggccg agcgcgggcg tgggggccgc ttcgagttcg acgggcgggg
     1381 ccgaggcgta gactccttcg ggcgtttgcg cggcgtcggg gcacgcgcgc cagccctcgc
     1441 ccgcgggcgg gatgctctgt ggccggcccg gcgtgacgaa ctccgtgtcg tccgtgacgg
     1501 ggcacgcgtc ctcgccacaa cccgagtcga ggacttcgac ggcgaccgtg tcgtgcgagt
     1561 cttcgtcgtg gtgcaggcag agcaggacgc aggcgatgaa cccccacaac agacacagcg
     1621 gcagcagcgt gccgaacatg cccgaaagtg gaaaccgcag acggttcatc gcccgaaagt
     1681 atacgcaatg gagaggagct ttgactagcc ggagtcgaag cggcctccgg cccgccgcgc
     1741 cctaccagcc gcggtgaatc tcccagcggt caacgacgcg cccgccctcg cagacgttgt
     1801 aatgcgcgtg ctgcgccgcc gtcaggcacg agtggttggc gagcacgcgc aggcgcgtcc
     1861 cgacacgcag cctgtcaaaa acgtctgcgt cgtccgcgac gacgacgccg tgctcctgcg
     1921 agagcgcatc gacgcgcagc cccgtgtccg cgccttcgag gtcgagcacg cgaccgtagc
     1981 cgcacgtcgc atcgatctcg accgcgccgc gatccttcga gagcgcgatg gcgcccgcgt
     2041 cgatgacgac cttgtttctc gtgcggtcgc ggtggacgac cgcggtgacg acggagaggg
     2101 cgcagtcttc gaacgcgcaa ctgccgaggg tggcctggaa ggcgtcgaag aagatgtaat
     2161 tgccgggccg cgcctcgtcc acgccgtcga ggtgatcgac ggcggtgacg gtcggcgtcg
     2221 agccgacgct gacgacgggg acttcgacgc ccgcgtcgcg cagccgcgcg gcgaagtcct
     2281 tcatcaggtc gcgctcgtgg cgcgcgaggc cgagcagttc ttcctgagag cgcgcgtggt
     2341 aggaatggcc cgcgtgcgtg aggatgcccg cgaagcggag gtgcggggcg tccgagatgc
     2401 ggcgcgcgat ctccagcgcc tcgggcgcgt gcggctcgac gccgcagcga tgataaccgc
     2461 agtccacctt catgaagagg tcgagcgtga cgccggctcg tcgcgcggcg acgtcgagca
     2521 cgggcggaat ctccacgtcg tcggtgagca ggtaaagccg ctcgcaaagc ttcgacaact
     2581 caatcgccac ttcgaacttg cccggctcga tgggcacggc gtaggtgatg tccgtgaagc
     2641 cgtgcgcggc gaaggcgcgc gcctcggcga gcgtcgagac ggtgatgccg cccgcgtgcc
     2701 cggcggtctg gatgcgcgcg acctcgacgc acttgtgcgt cttcacgtgc gggcgcagcg
     2761 aggcgccgaa ctgcttgacg cgcgagccga tgcgctcggc gttgcgcctg acgcgcgcgg
     2821 cgtcgaggac gagggaaggc gtcttgagtg tttgtaggtt catgtgtgtt gtgtgtgtgg
     2881 ggatgataac tcagaagcga gtgagggaaa acaggaacgg ccttgaactg gaagtgaggc
     2941 gcggatgacg attgtggggg gag
//
LOCUS       DBNGOH010000385         2542 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_58297, whole genome shotgun sequence.
ACCESSION   DBNGOH010000385 DBNGOH010000000
VERSION     DBNGOH010000385.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2542)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2542)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2542
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_58297"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(141..1331)
                     /locus_tag="AC159P_07660"
     CDS             complement(141..1331)
                     /locus_tag="AC159P_07660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HIR3695366.1"
                     /translation="MRLRLLLAAIVLLTGCVGAGASGALSESRGAGRVDAAAQEGGRV
                     ERQFNLGAGKRLDVKLKTGGAVDITGWDNDTVTVSASVSGENAADTDLSFNETAAGLQ
                     IGSRYTGPRRRSYNSDVRLEIKVPRRTDILLETMGGDIKLNGVEGDMRGQTMGGRLDL
                     SDLKGTLNLTTMGGGITLKQSQVNGKVSTMGGEVLLEDVTGDVKGSSMGGKVTYKNVT
                     NSKGETTGQEVKITSMGGEINVDTAPAGADVSTMGGDIDIRSAGGFVKANTMGGDIRL
                     GAVDGGIKATTMGGDVNARMVGNPDDGQRDVTITSMGGDIFLTVPAGLSMDIQIEVAR
                     TRNSAKTPQIVSDFPLQQRESDEWMADHGTPRKVTYGTGQVGGGRNKITIKTINGDVH
                     LKKG"
     gene            1595..>2542
                     /gene="hemE"
                     /locus_tag="AC159P_07665"
     CDS             1595..>2542
                     /gene="hemE"
                     /locus_tag="AC159P_07665"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020465628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HIR3695367.1"
                     /translation="MSQNTTTIENAAARDALGDSPFMRACRREPVPYTPVWLMRQAGR
                     YMREYREVRARTSFLELCKTPELAAEVTVYAAERLKVDAAIIFADILLIVEPMGLSLE
                     FSKGEGPVIHNPVREVRDVERLREVEDVGALSYVYEAIKITRGSLPAGVPLIGFCGAP
                     FTLASYIVEGGASRNYVHTKRLMYRDPGAWHALMSLIARALARYLNAQVAAGAQAVQL
                     FDSWVGCLSPDDYRDYVLPHTREVIRGVTPGTPVIHFGTGTAALLEPMREAGGDVIGL
                     DWRVRLDEAWARLGDVAVMGNLDPVTLFTSPELVREQAKK"
BASE COUNT          367 a          944 c          768 g          463 t
ORIGIN      
        1 cttgaccacc catatcaatt gcggattgcg gattgcggat ctcggattgg cttgctgccc
       61 gatcttcaag ctcgatgtca gccgttcaac ttccggcgcc cgaccaatcc gcaatccgcg
      121 atccgaagtc cgaaatccta tcatcctttt ttcagatgca cgtcgccgtt gatcgtcttg
      181 atggtgatct tgttgcgccc gccgccgacc tgccccgtcc cgtaagtgac cttgcgcggc
      241 gtgccgtggt cggccatcca ctcgtcggac tcgcgctgct ggagggggaa gtcgctgacg
      301 atctgcggcg tcttcgcgct gttgcgcgtg cgcgcgacct cgatctggat gtccatcgag
      361 aggcccgccg gcaccgtcag gaagatgtcg ccgcccatcg aggtgatggt cacgtcgcgc
      421 tgcccgtcgt cggggttgcc gaccatccgc gcgttcacgt cgccgcccat cgtcgtcgcc
      481 ttgatgccgc cgtccaccgc cccgaggcgg atgtcgccgc ccatcgtgtt cgccttgacg
      541 aacccgccgg cggagcggat gtcgatgtcg ccgcccatcg tcgaaacgtc ggccccggcg
      601 ggcgccgtgt ccacgttgat ctcgccgccc atcgaggtga tcttcacctc ctgccccgtc
      661 gtctcgccct tcgagttggt gacgttcttg tacgtcacct tgccgcccat cgaagagcct
      721 ttcacgtcgc ccgtcacgtc ttcgagcagc acctcgccgc ccatcgtcga gaccttgccg
      781 ttgacctgcg actgcttgag cgtgatgccg ccgcccatcg tcgtgaggtt gagcgtgccc
      841 ttcaagtccg agaggtcgag acggcccccc atcgtctgac cgcgcatgtc gccctcgacg
      901 ccgttgagtt tgatgtcgcc gcccatcgtt tcgagcagga tgtcggtgcg gcgcggcacc
      961 ttgatttcga gtcgcacgtc cgagttgtag ctgcggcggc gcgggccggt gtagcgcgaa
     1021 ccgatttgca gccccgcggc tgtctcgttg aagctcaggt cggtgtccgc cgcgttctcg
     1081 ccgctgaccg aggcggagac cgtgaccgtg tcgttgtccc agcccgtgat gtccaccgcg
     1141 ccgcccgtct tcagcttcac gtcgaggcgc ttgcccgccc cgaggttgaa ctgcctttcg
     1201 acgcgcccgc cttcctgcgc cgcggcgtcc acgcgccccg cgccgcgcga ctcggatagc
     1261 gcgcccgacg cgcccgcccc cacgcaaccc gtcagcagca caatcgcggc cagcaacaac
     1321 ctcagcctca ttttcgcctt cctcctcaac gttttctcca accccgtccc gcggcccggc
     1381 gcgccctcgg aaactcccga tccgccgtct tcacactcta tacaccgcgc gcgcaagttt
     1441 cgtgacagga aaatttagcc ccgccggccc ggctccggcg ccggggccgc taccgcaagc
     1501 cgcgcgcgat attgtagtct gtcccgtcga atccgcatta tcgggcggcg acgcggaatc
     1561 actttcgaac ccgacggaca ccgaaatcgg acggatgagc caaaacacca cgaccatcga
     1621 gaacgccgcc gcgagggacg cgctcggcga cagtcctttc atgcgcgcgt gccggcgcga
     1681 gcctgtgcct tacacgcccg tctggctgat gcggcaggcc gggcgctaca tgcgcgagta
     1741 ccgcgaggtg cgcgcgcgca cttccttcct cgaactctgc aagacgccgg agctggccgc
     1801 ggaggtcacg gtctacgcag ccgagcggct gaaggtggac gccgcgatca tcttcgccga
     1861 catcctgctc atcgtcgagc cgatgggcct gagtctcgaa ttctccaaag gcgaggggcc
     1921 cgtcatccac aaccccgtcc gcgaggtacg ggacgtggag cgcctccgcg aggtcgagga
     1981 cgtgggcgcc ctatcttacg tctacgaggc gatcaaaatc acgcgcggct cgctccccgc
     2041 aggcgtcccg ctcatcggct tctgcggcgc gcccttcacg ctcgcctcct acatcgtcga
     2101 gggcggcgcc tcgcgcaact acgtccatac caagcgcctg atgtaccggg accccggcgc
     2161 ctggcacgcg ctgatgtcgc tcatcgcgcg cgcgctcgcg cgctacctca acgcgcaggt
     2221 cgccgcgggc gcgcaggccg tccagctctt cgactcgtgg gtcggctgcc tctcgcccga
     2281 cgactaccgc gactacgtcc tgccgcacac gcgcgaggtc atccgcggcg tcacgcccgg
     2341 cacgcccgtc atccacttcg gcacgggcac ggcggcgctc ctggaaccga tgcgcgaggc
     2401 gggcggagac gtgatcggcc tcgactggcg cgtgcgcctc gacgaggctt gggcgcggct
     2461 cggcgacgtg gccgtcatgg gcaacctcga ccctgtcact cttttcacca gccccgaact
     2521 cgtccgcgag caggcgaaaa ag
//
LOCUS       DBNGOH010000386         8146 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_791167, whole genome shotgun sequence.
ACCESSION   DBNGOH010000386 DBNGOH010000000
VERSION     DBNGOH010000386.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8146)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8146)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8146
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_791167"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..184
                     /locus_tag="AC159P_07670"
     CDS             <1..184
                     /locus_tag="AC159P_07670"
                     /inference="COORDINATES: protein motif:HMM:NF024830.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4113 domain-containing protein"
                     /protein_id="HIR3695368.1"
                     /translation="AQATLPLERLPVRRCGLDPDVRLAGVNPKGRWRTRAARRSPRYT
                     TKLAEALLVKEPPAVR"
     gene            301..726
                     /locus_tag="AC159P_07675"
     CDS             301..726
                     /locus_tag="AC159P_07675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695369.1"
                     /translation="MAKSRQATPVTLTEWQRERLEHLWFVFGNHGPKTTPGNHQFIQG
                     LLEHGIDLRPLQTKRTARRDVPTGECVAAVEQVLSSKEGGEEGAARSGRLRLVPSPDP
                     GRRTQAPDPELKRMLDEMRERLTATRGRGGIGAGGGDAA"
     gene            774..977
                     /locus_tag="AC159P_07680"
     CDS             774..977
                     /locus_tag="AC159P_07680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695370.1"
                     /translation="MNERQQAHVTYFLERVEYWRGLGREYWPARMLAARDADHRHPLR
                     SEEEGSEVFDHLLRLQDEQAAVK"
     gene            complement(980..1465)
                     /locus_tag="AC159P_07685"
     CDS             complement(980..1465)
                     /locus_tag="AC159P_07685"
                     /inference="COORDINATES: protein motif:HMM:NF016811.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HIR3695371.1"
                     /translation="MGRGRQGNRVLHGLHRPRLRGLGLALQPPDAGGRRRPLRRRRVD
                     GVMLVRDRVCGMELTEADVKEPLVHEGRTYFFCSSGCRAEFERHTDEYAEAARPEGRR
                     EEMCRFLRYVGPELPLAELLYRPQHSLILQSYKAKERKEPLNGDGFGVGCYTPASGAG
                     G"
     gene            complement(1351..1626)
                     /locus_tag="AC159P_07690"
     CDS             complement(1351..1626)
                     /locus_tag="AC159P_07690"
                     /inference="COORDINATES: protein motif:HMM:NF039498.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5676 family membrane protein"
                     /protein_id="HIR3695372.1"
                     /translation="MASNIRALTITGAVMAAVTYVICAAFVALAPGVATSLGSDITHI
                     DLSTVGRAVTWGGAVRGIVFFTVFIALVCAASGWLYNRLTRAADVGH"
     gene            complement(1685..1894)
                     /locus_tag="AC159P_07695"
     CDS             complement(1685..1894)
                     /locus_tag="AC159P_07695"
                     /inference="COORDINATES: protein motif:HMM:NF023095.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2933 domain-containing protein"
                     /protein_id="HIR3695373.1"
                     /translation="MNHQHVSPITGRRRSRLAVALLVFLAVAGLFLFREHQSHVLRLL
                     PYLLLLACPVVHLFMHRGHGGGRHH"
     gene            complement(1910..2470)
                     /locus_tag="AC159P_07700"
     CDS             complement(1910..2470)
                     /locus_tag="AC159P_07700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695374.1"
                     /translation="MFAGTIKTPNEVGGAEPFLLSRCQGLTAARRCPSCGEELSDRFG
                     FCPMDGTPLDASRIVEGRDDFRLTVVEHRALPVRLLAALFEAAHAGLRAEAQSERLSG
                     PNARAAAEAVGRMAERHEEVCFAMLEDAGLLRRLAGELSAFARDSRLTWPEFRRDPAG
                     FIRRAFDALDTLGLIPFSRRHQTHRF"
     gene            complement(2658..3557)
                     /locus_tag="AC159P_07705"
     CDS             complement(2658..3557)
                     /locus_tag="AC159P_07705"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HIR3695375.1"
                     /translation="MKTGALFLTLALLAAGAAVGPAAARQGDAGRGPQTQEADERPLV
                     VNADLVTVTVTVTDGEGRHVAGLGKSAFAVTDDKVPQEVSFFADEDEPTSVGVLLDVS
                     GSMSGDKIERAKEALAGLVRTSHPMDEFFLIDFDSQARLILNRTRDGDAVLAKFTYAR
                     PHGETALYDAVYLGVEKVMRGAHRKRAVIVISDGEDNSSRHSFGELRRQLQESGVALY
                     AVGVSENWLPKQGVGEETLRRLASVTGGEAFFPDGAAGVSEAFERIALILRHQYAIGY
                     RPSNFAADGRWHRLRVGSRRRPG"
     gene            complement(3598..4635)
                     /locus_tag="AC159P_07710"
     CDS             complement(3598..4635)
                     /locus_tag="AC159P_07710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695376.1"
                     /translation="MRKLIFNLFAAGVVSLFIAGTAAAQTEYRFKVKHDHVRKYCAGE
                     LVITDSGVEYVTEERGHSRNWQYADVKMLKLVSPTKLKVLSYENGRFKVGDRDFSFEL
                     TEGEITREVSDFVLSRVKRPVSTSFIPDREDEPAYSIPARHRRRLGGDEGVIKVYADG
                     VVYESGGGKEVSRYWRWSDIQGVGRSGQFRLSVTTYEPEFGGPTKSFDFDLKEALTDE
                     TYDYVWERVYKVSARLPSASEVKTLARAPVQEKRAPRKETSGEVTVVGYVSDSNCGLK
                     HHEGMDETDCVLACAKGGRFVLADRENKRVYDLDDGAQDAAKEFANKKVRITGHLMGQ
                     GIHVMKIEPES"
     gene            complement(4762..5709)
                     /locus_tag="AC159P_07715"
     CDS             complement(4762..5709)
                     /locus_tag="AC159P_07715"
                     /inference="COORDINATES: protein motif:HMM:NF013618.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008933 - peptidoglycan lytic
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HIR3695377.1"
                     /translation="MSYITIPGKAAAYARRAIALVLLAAALGGGWEARGQSADAEEDD
                     PVARAHRRIELAVGARAADAERVKAGSVALEGAVAARKQGDRKRAGELLSEAERLTAA
                     EHQAERSALIDDLYRSIASERDALSPKKASATETIAGSRGLPVYVTAAAVPRDAIAAV
                     NGYRSTLGRILQEENVPVELLAVAYVESRFKTGAVSPKGAGGIWQFMPGTAARYGLQV
                     TAMVDHRTHPDHSTRAAARYLRDLHRMFGDWELALAGYNWGEGNVQKAIRRAGTRDFD
                     ELARRGFIPLETRRYVPAVLALASRVGGAGGGGLVAVGR"
     gene            complement(5728..>8146)
                     /locus_tag="AC159P_07720"
     CDS             complement(5728..>8146)
                     /locus_tag="AC159P_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR3695378.1"
                     /translation="LDVIERVRARLEQLKPSLPEGVEVVPTYDRGELIERSIETLTGA
                     LTEELIVVSLIIIIFLLHFPSAAVPVITIVSAVVISFIPMFGLDVTANIMSLAGIAIA
                     VGAMVDAAIVVVEQTHKKLEDWEAAGEPGNYKDAIVAAAREVGAPSFYSLLVIAVAFI
                     PIFALEAQEGRLFKPLAFTKNMAMAAAAVLSITLVPALTVLFIRRKKFSFRPRPLARL
                     ANFFLVGKIYNEDKHPLSRVLMRVYEPVVRLVLRFKWTTVLAAVLLVALTVPVYLRLG
                     SEFMPPLDEGVLLYMPTTMPGISVTEAGRLLQVQDRIIRTFPEVERVFGKAGRAETAT
                     DPAPFSMMETVVVLKPQSEWPKVERWYSGRAPEWLAGVLRRAWPDHKSTQELIYGPGG
                     LDEATRLPGVVNAWTMPIKGRVDMLSTGIRTPVGIKVMGSNIRQIEELGLHIEMALKD
                     VRGTKSVFAERTAGGYFLDFDFKREALARYGLSIDEANAVVMSAVGGENVTTTVEGRE
                     RYPVNVRYLRNYRSELDRLGRVLVPTMSGAQIPLAELADISVRSGPGMIRDENGRLSG
                     YVFIDVGDRDIGSYVEEAKRVVAEHVKLPPGYMLHWSGQYENMERVAERLIVVVPITV
                     FIIFLLLYFNTKSLTKTMIVFLAVPFSAVGAVWLLYFLDYNMSVAVWVGLIALLGVDA
                     ETGVFMLLYLDLSYDDAKSKGRMRTKEHLNEAIVHGAVKRLRPKFMTVAVDFIGLVPV
                     MWAVGAGSDVMKRIAAPMVAGMFTSFIMELVVYPAIYAIWKWHAEVKKEAEGLPAGLP
                     AIPAVGS"
BASE COUNT         1331 a         2784 c         2660 g         1371 t
ORIGIN      
        1 cgcgcaagcg actctgcctc tcgagagact tcccgtccgc cggtgcggtc tcgacccaga
       61 cgtgcggctc gccggggtga acccgaaagg caggtggagg acgagggcgg ctaggcgcag
      121 cccgcgctac acgaccaagc tggcggaggc gctgctggtc aaagaacctc ccgcggtcag
      181 atgaggtcac ggcaccgacc gccgataccg cgtcgaattg agtcttgagt gttcgcgccg
      241 gcgggcatac atcgatcgag tagcgtcgaa cgaagggccc ggaaaggtac gataccccag
      301 atggcaaaga gcaggcaggc gacccccgtc acgctcacgg aatggcagcg cgagcgactc
      361 gagcatttgt ggttcgtctt cggcaaccac gggccgaaga ccacgccggg gaatcatcag
      421 ttcatccagg ggctcctcga gcacgggata gacctgcggc cgctgcagac gaagcggacg
      481 gcgaggcgtg acgtcccgac cggcgagtgc gtggccgccg tcgagcaggt cctgtcctcg
      541 aaggagggcg gcgaagaagg ggccgcacgc tccggccgcc tgaggctggt cccctcgcca
      601 gaccccggac gtaggacgca ggcgccggac ccggagctga agaggatgct cgacgagatg
      661 cgcgaacggc tcaccgccac ccgcggccgc gggggaatcg gcgccggcgg cggggacgct
      721 gcttagaggc accggccgcc gggcggtgag gcaagcgcta taactacccc cttatgaacg
      781 agagacagca ggcgcacgtc acctacttcc tggagcgggt cgagtactgg cgggggctcg
      841 ggcgggagta ctggcctgcg cggatgctcg cggccaggga cgcagaccac agacaccccc
      901 tgcgcagcga ggaggagggg tcggaggtct tcgaccacct gctcaggctc caggatgagc
      961 aggcggcagt caagtgacct cacccgccgg cgccggacgc gggcgtgtaa cagccgacgc
     1021 cgaagccgtc gccgttcagc ggctccttcc gttccttcgc cttgtagctc tggaggatca
     1081 gggagtgctg cgggcgatag agcagttccg ccagcggcag ctccggcccc acgtagcgga
     1141 ggaacctaca catctcttcc cgcctcccct cgggacgggc cgcttcggca tactcgtcgg
     1201 tgtgccgctc gaactcggcc cggcatcccg aggagcagaa gaagtacgtc ctgccttcgt
     1261 ggacgagcgg ctctttcacg tcagcctcgg ttaactccat cccgcataca cgatccctaa
     1321 cgagcatgac tccgtccacc cgccgccgcc tcagtggccg acgtcggccg cccgcgtcag
     1381 gcggttgtag agccagcccg aggccgcgca gacgagggcg atgaagaccg tgaagaacac
     1441 gattcccctg acggccccgc cccatgtcac cgccctgcct acggtcgaga ggtcgatgtg
     1501 tgtgatgtcg gagccgagac tggtcgccac gccgggggcc agcgcgacga aggcggcgca
     1561 gatcacatag gtcaccgcgg ccatcaccgc cccggtgata gtcagtgcgc gaatgttact
     1621 tgccatgaca gactctcctt tctcttcact tgcgtgtatg tctcgccggg ccctaccggc
     1681 gaggttagtg gtgcctgccg ccgccatgcc cccggtgcat gaagagatgc acgacggggc
     1741 aggcgagcag caggaggtat ggcagaaggc ggagcacgtg cgactggtgt tccctgaaaa
     1801 ggaagagccc agcgacggcg aggaagacga ggagtgcgac ggcgaggcgt gaacgccgcc
     1861 tgccggtaat cggcgaaacg tgttgatgat tcataactcc tcccggacgc taaaacctat
     1921 gtgtctggtg gcgacgcgaa aaaggtatca atcccagtgt atccagggcg tcaaaggctc
     1981 tccggataaa ccccgccggg tcgcgccgga actcgggcca ggtcaggcgc gagtctcgcg
     2041 cgaacgcact cagttcgccg gccagccgcc gcaagagccc cgcgtcctcc aacatggcga
     2101 agcacacctc ttcgtgccgc tcggccatcc gtccgacggc ttcggcagcc gctcgggcgt
     2161 tcgggccgct caggcgttcg gactgcgcct ccgctctcag cccggcgtgt gccgcctcga
     2221 acagcgccgc gagtagtctt acggggagcg ccctgtgctc gacgaccgtt aggcggaagt
     2281 catcgcggcc ctcgacgatc cgggatgcgt cgagcggcgt accgtccatg gggcagaagc
     2341 cgaacctgtc cgatagctcc tcgccgcagg acggacacct ccgggccgcg gtcaagcctt
     2401 gacaacggct caagagaaac ggctcggccc cgccgacctc attcggcgtt tttatcgttc
     2461 cggcaaacat cctctcacct tcataagacc gccctccgct cgcggaaggc ttcgctaacc
     2521 ggggcggggt caaaatcccc tgccgtgacg gcactggctt ggggccgccc cggcggactg
     2581 ccgcctttca tcccccaatc aacggttccg acccgggccg gcgtaataac cttctttcgc
     2641 gcggacgtgt aggcgtttca ccccgggcgg cggcgtgacc ctaccctgag gcgatgccac
     2701 ctgccgtcgg ccgcgaagtt cgacggccgg taacctatgg cgtactgatg acgcaaaatt
     2761 aaagcgatgc gctcgaacgc ctcgctcacc ccggccgcgc cgtcggggaa gaacgcctcg
     2821 ccgcccgtca cggaggccag ccgcctcagc gtctcctcgc ccacgccctg tttcggcagc
     2881 cagttctccg agacgccgac ggcgtagagg gcgacgcctg actcctgcaa ctgccgcctg
     2941 agctcgccga agctgtggcg cgagctgttg tcctcgccgt cgctgatgac gatgaccgcg
     3001 cgtttccgat gagcgccgcg catgactttt tccaccccga ggtacacggc gtcgtagagc
     3061 gccgtctcgc cgtgcggccg tgcgtaggtg aacttcgcca gcacggcgtc gccgtcgcgg
     3121 gtcctgttca agatgagcct ggcttgtgag tcgaagtcga tgaggaaaaa ttcgtccatc
     3181 gggtggctcg tcctgacgag tccggcgagg gcctctttgg cgcgctcgat cttgtcgccg
     3241 ctcatcgagc ccgacacgtc caagagcacg ccgacgctcg tcggctcgtc ctcgtcggcg
     3301 aagaagctga cctcctgagg gaccttgtcg tccgtcacgg cgaaggcgct cttgcccagc
     3361 cccgcgacgt ggcgtccctc gccatccgtc accgtgaccg tgaccgtcac gaggtcggcg
     3421 ttgacgacga ggggccgctc gtccgcctcc tgcgtctgcg gtcccctgcc cgcgtctccc
     3481 tgccgcgcgg ccgcaggccc gacggcagct cccgcggcca gtagtgcgag ggtaagaaac
     3541 agcgccccgg tcttcatacg tcacctccgc gccccccgga ccgatgcccc gcggtaatca
     3601 ggactcgggc tcgatcttca tcacgtgaat cccttgaccc atcaggtggc cggtgattct
     3661 gaccttcttg ttggcgaact ctttggccgc gtcttgcgcc ccgtcgtcga ggtcgtagac
     3721 acgtttgttc tcgcggtcag cgaggacgaa cctcccgccc ttggcgcacg ccaggacgca
     3781 gtctgtctcg tccatgccct cgtggtgctt gaggccgcag ttggagtcgc tgacgtagcc
     3841 gaccaccgtc acctcgcccg acgtctcctt ccgcggcgcg cgcttctcct gcacgggcgc
     3901 tctcgctaaa gtcttcacct ccgaggcgga gggcaggcgg gcgctcacct tgtagaccct
     3961 ctcccacacg tagtcgtagg tctcgtcggt tagggcttcc ttcaggtcga aatcgaagga
     4021 cttcgtgggg ccgccgaact cgggttcgta ggtcgtgacc gagagtctga actgccccga
     4081 gcggccgacg ccctggatgt ctgaccagcg ccagtagcgc gagacctctt tcccgccccc
     4141 ggactcgtag acgacgccgt cggcgtaaac cttaatgact ccttcgtccc cgccgagcct
     4201 ccggcggtgg cgcgccggga tggagtaggc gggctcgtct tctctgtcgg ggatgaacga
     4261 ggtcgagacc ggcctcttca ccctcgacag gacgaagtcg ctcacctccc ttgtgatctc
     4321 gccctccgtc aactcgaagc tgaagtcgcg gtcacccacc ttgaacctgc cgttctcgta
     4381 tgagagcacc ttcagcttcg tgggcgagac gagcttaagc atcttgacgt cggcgtactg
     4441 ccagttccgg gagtgcccac gctcttccgt cacgtactcc acgcccgagt cagtgatgac
     4501 cagctcgccc gcgcagtatt tcctgacgtg atcgtgcttg accttgaagc ggtactcggt
     4561 ttgcgcggcg gccgtacccg cgatgaagag ggagacgact cccgccgcga acaggttgaa
     4621 aattagcttc ctcatgattg cctccgaacc tttcatgcgg gcgcgccctc cggcggtttc
     4681 cgattgggca tttcgcgcgc gtgtgattct tttcgcccct ccatttttca tctcttcacc
     4741 tccggcccct ccgccgcagg gtcagcggcc gaccgccacc aagcccccgc cgcctgcgcc
     4801 ccccaccctc gacgcgaggg ccagcaccgc cggcacgtac ctgcgcgtct cgagtggaat
     4861 aaagccgcgc cgcgccagct cgtcgaagtc gcgcgtgccc gcacgcctga tcgccttctg
     4921 gacgttgcct tcgccccagt tgtagccggc cagcgcgagc tcccagtcgc cgaacattcg
     4981 gtggaggtcg cgcaggtagc gggcggcggc gcgcgtcgag tggtcggggt gcgtgcggtg
     5041 gtcgaccatc gccgtcacct gcaggccgta gcgcgcggcg gtgccgggca tgaactgcca
     5101 gatgccgccc gcgcctttcg gcgagaccgc ccccgtcttg aaacggctct cgacgtaggc
     5161 cacggcgagc agctcgacgg ggacgttctc ctcctggagt atccgcccca gcgtcgagcg
     5221 gtagccgttg acggcggcga tggcgtcgcg aggcaccgcc gcggccgtca catagactgg
     5281 cagcccccga ctgccagcga tagtttcggt agcggacgct ttcttcgggc tgagggcgtc
     5341 gcgctccgac gcgatagagc ggtagagatc gtctatgagc gcgctgcgct ctgcctggtg
     5401 ctccgcggcc gtgagcctct cggcctcgct caggagctcg ccggcccttt ttcggtcgcc
     5461 ctgcttccgc gccgccaccg ccccttcgag cgcgacggag ccggccttga cccgctccgc
     5521 gtctgccgcg cgcgccccga ccgcgagctc gatcctccgg tgagcgcggg cgactgggtc
     5581 gtcttcctcg gcatccgccg actgcccgcg ggcttcccac ccaccgccga gcgccgccgc
     5641 aagcaggaca agggcaatgg ctcgtctcgc gtacgcggcg gccttgccgg ggatagttat
     5701 gtatgacatg aggcatgaca tcttttctca gctccctacc gcgggaattg ccggcagtcc
     5761 cgccggcaac ccttccgcct ctttcttcac ctccgcgtgc cacttccaga tggcgtagat
     5821 ggccgggtag acgaccagct ccatgatgaa ggaagtgaac atcccggcga ccatcggcgc
     5881 cgcgatcctc ttcatcacgt ccgagccggc ccccaccgcc cacatgacgg ggacgaggcc
     5941 gatgaagtcc accgcgaccg tcatgaactt gggccgcaga cgcttgaccg caccgtggac
     6001 gatcgcctcg ttgaggtgct ccttcgtcct catccgcccc tttgacttcg cgtcgtcgta
     6061 ggagaggtcc aggtagagca gcatgaagac cccggtctcg gcgtccacgc ccaggagcgc
     6121 gatgagcccc acccagaccg ccacgctcat gttgtaatcg aggaagtaga gcagccagac
     6181 ggcgccgacg gccgagaacg ggaccgcgag gaagacgatc atcgtcttcg tcaaggattt
     6241 ggtgttgaag tagagcagca ggaaaatgat gaagaccgtg atgggcacga cgacgatgag
     6301 ccgctctgcc acccgctcca tgttctcgta ctgcccgctc cagtggagca tgtagcccgg
     6361 cgggagtttc acgtgttcgg cgacgacgcg cttcgcttcc tcgacgtagc tcccgatgtc
     6421 gcggtcgccc acgtcgatga agacgtagcc ggagaggcgt ccgttctcgt cgcggatcat
     6481 gccggggccg gagcgcacgc tgatgtcggc caactcggcg agcgggatct gcgccccgct
     6541 catcgtcggg acgagcacgc gtcccagccg gtcgagctcg ctccggtagt tccgcaggta
     6601 gcggacgttg acggggtagc gctcgcgccc ctcgaccgta gtggtgacgt tctcgccgcc
     6661 gacggccgac atgacgacgg cgttcgcctc atcgatactc agcccgtagc gagccagtgc
     6721 ttcgcgcttg aagtcgaagt cgaggaagta gccgccggcc gtgcgctcgg cgaagacgct
     6781 cttcgtgccg cggacgtcct tcaaggccat ctcgatgtgc aggcccagct cctcgatctg
     6841 acggatgttg gagcccatga ccttgatgcc gaccggcgtg cggatgcccg tcgagagcat
     6901 gtccacgcgc cctttgatcg gcatcgtcca ggcgttgacg acccccggca gccgcgtcgc
     6961 ctcgtccagg ccgccgggtc cgtagatcag ctcctgcgtg ctcttgtggt cgggccacgc
     7021 ccggcgcagc acgcccgcca gccactccgg cgcgcggccc gagtaccacc gctcgacctt
     7081 cggccactcg gactggggct tcagcacgac gaccgtctcc atcatcgaga agggcgcggg
     7141 gtctgtggcc gtctcggcgc ggcccgcctt gccgaagacg cgctccacct cggggaacgt
     7201 gcggatgatc ctgtcctgca cctggaggag gcggccggcc tcggtgacgg agatgcccgg
     7261 catcgtcgtc ggcatgtaga gcagcacgcc ctcgtcgagc gggggcatga actcggagcc
     7321 gaggcggagg tagacgggga cggtgagcgc gaccaggagg acggcggcga ggacggtcgt
     7381 ccacttgaac cgcagcacca gccgcaccac gggctcgtag acgcgcatca gcacgcggct
     7441 taaggggtgc ttgtcctcgt tgtagatctt cccgacgagg aagaagttcg cgaggcgcgc
     7501 gaggggccgg ggccggaagc tgaacttctt cctgcggatg aagagcacgg tgagcgcggg
     7561 cacgagcgtg atggagagga cggcggccgc cgccatcgcc atgttcttcg tgaacgcgag
     7621 cggtttgaag agccgcccct cctgcgcttc gagcgcgaag atggggatga aggcgaccgc
     7681 gatcacgagc agggagtaga agctcggggc gcccacctcc ctggcggccg cgacgatcgc
     7741 gtctttgtag ttgccgggct cgccggcggc ctcccagtct tcgagcttct tgtgcgtctg
     7801 ctcgacgacg acgatggccg cgtccaccat cgcgccgacg gcgatggcga tgccggcaag
     7861 cgacatgatg ttggccgtca cgtcgaggcc gaacatgggg atgaaggaga tgacgaccgc
     7921 cgagacgatg gtgatgacgg ggacggccgc gctcgggaag tgaagcagga agatgatgat
     7981 gatcaggctg acgacgatca gctcttcggt gagcgcgccc gtgagcgtct cgatcgaccg
     8041 ctcgatcagc tcgccgcggt cgtaggtcgg gacgacctcg acgccctcgg ggaggctcgg
     8101 cttgagttgt tcgaggcggg cccggactcg ctcgatgacg tcgagg
//
LOCUS       DBNGOH010000387         2244 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3908933, whole genome shotgun sequence.
ACCESSION   DBNGOH010000387 DBNGOH010000000
VERSION     DBNGOH010000387.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2244)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2244)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2244
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3908933"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(21..1703)
                     /locus_tag="AC159P_07725"
     CDS             complement(21..1703)
                     /locus_tag="AC159P_07725"
                     /EC_number="3.2.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0031222 - arabinan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HIR3695379.1"
                     /translation="MKIKRREFVGGAVAAGAGVLTARAAGGFARSARAADSRVEVLVG
                     EEIGTISRDIYGHFAEHLGGVVYDGIWVGENSRVPNTGGIRTALVEAMKRIKAPVIRW
                     PGGCFADSYDWRDGTGPRARRPRRTNFWVDAPEWPKGAPDGPWKYETNHFGTNEFLRF
                     CQLTGAEAYLAANLRSLGAQQFFDWVEYVNSPAGSTTAAEQRAGSDTPSRDPYRVRFW
                     GIGNESWGCGGNFTPEEYAAEYRRFTAAVPRYGVDLKFIASGANVDDFNWTRGFFSKT
                     REKGAGLFNGIYGWGLHHYAWNVSQGRSNDWFGAKGDGLKFSDGEYYELLREGDRMES
                     LINQHWTIMGEYDRTHRVKLVVDEWGAWYKTGSEVHPSHLLGQQSTMRDALLAGLTLD
                     TFHRHADKVVMANVAQLVNCLHALFLAHEDKFVLTPTFHVFEMFIPHMGAKAVRAVFS
                     APEVQWTRADKPAEFWGLAGSASVNGKQLTLTVTNPHLTEAREAEIAVRGARVTEARA
                     RVLAEPDVHAHNTFQNPRAVEPRDEQVKTPTAGTLAYRFAPASVTRLLITLA"
     gene            complement(1726..>2244)
                     /locus_tag="AC159P_07730"
     CDS             complement(1726..>2244)
                     /locus_tag="AC159P_07730"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3695380.1"
                     /translation="TRALRRAAELDPKFGPPYAYLGMMRAKAGAYPEAVALYEDALRA
                     DPKLAPAHYLIAEALLKQTSADAARAESHLRRAVELDATFTPARLSLGRFYMRAERWD
                     AAVAELEKAARLDPDVAEAHYQLGRAYVRLKRQQDAQTALATFKRLSETQKAREDSEL
                     REVVKRLANVRF"
BASE COUNT          315 a          755 c          793 g          381 t
ORIGIN      
        1 ggcggagccg cgaggacgct tcacgccagc gttatcagca ggcgcgtcac agaggcgggc
       61 gcgaagcggt aggcgagcgt gcccgccgtc ggggtcttaa cttgctcgtc gcgcggctcg
      121 acggcgcgcg ggttctggaa cgtgttgtgc gcgtgcacgt ccggctcggc gagcacgcgc
      181 gctctcgctt ctgtgacgcg cgcgccgcgc acggcgatct cggcctcgcg cgcctcggtc
      241 aggtgcgggt tcgtcacggt cagcgtcaac tgcttgccgt tcaccgaagc cgacccggcg
      301 aggccccaga actcggcggg cttgtcggcg cgcgtccatt gcacttcggg cgcgctgaag
      361 acggcgcgca ccgctttcgc gcccatgtgc ggtatgaaca tctcgaagac gtggaaggtc
      421 ggcgtcagga cgaacttgtc ctcgtgcgcg aggaagagcg cgtgcaggca gttgacgagc
      481 tgcgcgacgt tcgccatcac gaccttgtcg gcgtggcgat gaaaggtgtc gagcgtgaga
      541 ccggcgagca gcgcgtctct catcgtcgat tgctggccga gcaggtgcga cggatgcacc
      601 tccgaccccg tcttgtacca agcgccccac tcgtccacga cgagtttgac gcggtgcgtg
      661 cggtcgtact cgcccatgat cgtccagtgc tggttgatga gcgactccat gcggtcgccc
      721 tcgcgcagca gttcgtagta ttcgccgtcg ctgaacttca gcccgtcgcc cttggcgccg
      781 aaccagtcgt tgctcctgcc ctgcgagacg ttccaagcgt agtgatgcag cccccagcca
      841 tagatgccgt tgaaaagacc ggcgcccttc tcgcgcgtct tcgagaagaa gccgcgcgtc
      901 cagttgaagt cgtccacatt cgcgcccgag gcgatgaact tgagatcgac gccgtagcgc
      961 ggcaccgccg ccgtgaagcg gcggtactcg gcggcgtact cctcgggcgt gaagttgccg
     1021 ccgcagcccc acgactcgtt gccgatgccc cagaagcgca cgcggtaagg gtcgcggctc
     1081 ggcgtgtcgc tgcccgcgcg ctgctcggca gcggtggtcg agccggcggg cgagttgacg
     1141 tactcgaccc agtcgaagaa ctgctgcgcg ccgaggctcc ggaggttcgc ggccagatag
     1201 gcttcggcgc cggtcaactg gcagaagcgc aagaattcgt tcgtcccgaa gtggttggtc
     1261 tcgtacttcc aggggccgtc gggcgcgccc ttcggccact cgggcgcgtc cacccagaag
     1321 ttggtgcggc gcgggcggcg ggcgcgcggg ccggtgccgt cgcgccagtc gtagctgtct
     1381 gcgaagcagc cgcccggcca ccggatgacc ggcgccttga tgcgcttcat cgcctcgacg
     1441 agcgccgtgc ggatgccgcc cgtgttcggg acgcgcgagt tctcgccgac ccaaatgccg
     1501 tcgtagacga cgccgccgag gtgctctgcg aagtggccgt agatgtcgcg gctgatcgtg
     1561 ccgatctctt cgccgacgag gacttcgacg cgcgagtcgg cggcgcgcgc cgaacgcgcg
     1621 aagccgcccg ccgctcgcgc cgtcaacaca ccagcaccgg cggcgaccgc gccgcctacg
     1681 aattcacgcc ttttgatttt cataatctgc ggtgcgatcc gaatttcaga aacggacgtt
     1741 ggcgagtcgc ttcacgacct ctctcaactc cgagtcctcg cgcgccttct gcgtctcgct
     1801 caggcgcttg aaggtggcga gcgcggtctg cgcgtcctgt tggcgcttga gccgcacgta
     1861 ggcgcgaccg agctggtagt gtgcttcggc cacgtcgggg tcgagacgcg cggccttctc
     1921 gagctccgcg accgccgcgt cccagcgctc ggcgcgcatg tagaacctgc cgagcgagag
     1981 gcgcgcgggc gtgaacgtcg cgtcgagttc gacggcccgc cgcaggtgcg actcggcgcg
     2041 cgccgcgtcg gcgctcgtct gtttgagcag cgcctcggcg atcaggtagt gggcgggggc
     2101 gagcttcggg tccgcgcgca gcgcgtcctc gtagagcgcg acggcctcgg ggtaggcgcc
     2161 cgccttcgcg cgcatcatgc cgaggtaggc gtagggcggg ccgaacttcg ggtcgagttc
     2221 ggcggcgcgc cgcagggcgc gcgt
//
LOCUS       DBNGOH010000388         2334 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_791454, whole genome shotgun sequence.
ACCESSION   DBNGOH010000388 DBNGOH010000000
VERSION     DBNGOH010000388.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2334)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2334)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2334
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_791454"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..390
                     /locus_tag="AC159P_07735"
     CDS             <1..390
                     /locus_tag="AC159P_07735"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR3695381.1"
                     /translation="TALLVRRRTIEDVGALDEGFHMYFEDVEWSLRMVRGGWHLVFEP
                     EAEVMHHGGQSALLRWGAGERWRVQLEASLKFQKQCLPAWQVRANLLASCAVLLFERV
                     SRKLHGRPADDVRTAFEVYLKHLRHAP"
     gene            413..1171
                     /locus_tag="AC159P_07740"
     CDS             413..1171
                     /locus_tag="AC159P_07740"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3695382.1"
                     /translation="MTKTIQTGVLKLTQRVRWRLGMRVAAAELEQGTDESVAAFYSNR
                     VTDCAFLDDPAHYEHPRARWVTERLSEGDGAGRGGRLLEVGCGNGGMTRLLSPLVDEV
                     VALDTSEPSLQALAALGLKNVKTANSLLEHFEPGALFDWIVVSEVIEHLRRPEDAVDR
                     CARWLAPGGAMLVTTPNGHWESDEHLHEFTLAAFAAMLARTGVESVHASYLRDRENRR
                     RWLVAELKAPTTRPAPDDFHDRRETARKRRSGHR"
     gene            1644..2168
                     /locus_tag="AC159P_07745"
     CDS             1644..2168
                     /locus_tag="AC159P_07745"
                     /inference="COORDINATES: protein motif:HMM:NF024120.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HIR3695383.1"
                     /translation="MAAQNVTSDDPRVNEWLNLKPYGAVGDGLADDGPALHAAFNALM
                     AAGGGTLFVPPGKFRIATTVSANFLNTASAVVIKGAGSASQLYVATGESASALELKNA
                     ESLLIDNLVFVGNPEAPTDAKIVLDIQGGFKAMIRNTIFYGLISQVAGGSIVHAQGRL
                     VPEHAKPRPAGEKR"
BASE COUNT          384 a          825 c          756 g          369 t
ORIGIN      
        1 accgcgctgc tcgtccgccg ccggacgatc gaagacgtgg gcgctctgga cgaaggcttc
       61 cacatgtact tcgaggacgt ggagtggagc ctgcggatgg tgcgcggcgg gtggcatctg
      121 gtcttcgagc cggaggcgga ggtgatgcat cacggcgggc agagcgcgct cttgcggtgg
      181 ggcgcgggcg agcgctggcg cgtgcagctc gaagcgtcgc tgaagttcca gaagcagtgc
      241 ctgcccgcgt ggcaggtgag ggcgaacctg ctggcgagct gcgccgtgct tctgttcgag
      301 cgcgtctcgc gcaagctcca cgggcgcccg gcggacgacg tgcgtaccgc gttcgaagtt
      361 tatctgaaac acctgcggca cgcgccgtga gaaagccggt gattccgaga tggtgacgaa
      421 gacgatacag acgggcgtgt tgaagctcac gcagcgcgtg cgctggcgtc tggggatgcg
      481 cgtggccgcc gccgagctgg agcaggggac ggacgagtcg gtcgcggcct tctacagcaa
      541 ccgcgtcacg gactgcgcct tcctcgacga ccccgcgcac tacgagcacc cgcgcgcgcg
      601 gtgggtgacg gagcgtctga gcgagggcga cggcgcgggt cgcggcggtc ggctcctcga
      661 agtcggctgc ggcaacgggg gcatgacgcg cctgctctcg ccgctcgtcg atgaagtcgt
      721 cgcgctcgac acttcggagc cttcgcttca ggcgctcgcg gcgctcggcc tgaagaacgt
      781 gaagacggcg aacagcctgc tcgaacactt cgagccgggc gcgctcttcg actggatcgt
      841 cgtctccgaa gtgatcgaac acctgcgccg gcccgaggac gccgtggatc gctgcgcgcg
      901 ctggctcgcg cccggcggcg cgatgctcgt gacgacgccc aacggccact gggagtcgga
      961 cgagcacctg cacgagttca cgctcgccgc cttcgccgcg atgctcgcgc gcaccggcgt
     1021 cgagtcggtg cacgcctcgt acctgcgcga ccgagagaac cgccgccgct ggctcgtcgc
     1081 cgagctgaag gcgcccacga ctcgccccgc gcccgacgac ttccacgacc gccgcgagac
     1141 ggcgcgcaaa cgaaggtcgg gccacaggtg agcgccgccg tcgaatctcg ccgcgggccg
     1201 cacgcacttc tcacgccccg tcaacacaac tgatagcgac ggcccttcgc gcgccgccgc
     1261 cttcccgcgt tcgacgccgg ctccgcaaaa cttgtcggcc ccggcgccgg gccgcgcgtc
     1321 taaaaaaaaa tacgtacgcg cgcgtacgcg aaatgttact tcaagagatg tcgtcccgct
     1381 gcgacttacg gcaggcccgt tcaggctcgc ccgccgaagc cctgaatccc ggcccgtcac
     1441 ctcaaacctc gtgagtcgag tcggaggaca gcagcccccc tcagaagcca ttttcaaggc
     1501 tttcatccga ccataaccga tcaatattaa cttcgagcgc cgttcgccgt gtctcgtccg
     1561 ggcgcccgtc tatccggcgg actatgcacc ccctgagaaa aactcttctc attatcttac
     1621 tcgcgctgtc cggctcgctg accgtggccg cgcagaacgt gaccagcgac gacccgcgcg
     1681 tgaacgagtg gctgaacctc aagccgtacg gcgcggtcgg cgacggcttg gccgacgacg
     1741 gccccgccct gcatgccgcg ttcaacgcac tgatggcggc gggcggcggc acgctcttcg
     1801 tcccgccggg caagttccgc atcgccacca ccgtctcggc caacttcctg aacacggcct
     1861 cggcggtcgt catcaagggc gccggctcgg cctcgcagct ctacgtcgcc acgggcgagt
     1921 cggcctcggc gctcgaactg aagaacgccg agagcctgct catcgacaac ctcgtcttcg
     1981 tcggcaaccc ggaggcgccg accgacgcca aaatcgtgct cgacattcag ggcggcttca
     2041 aggcgatgat ccgcaacacg atcttctatg gcctcatctc gcaggtcgcg ggcggctcca
     2101 tcgtccacgc gcaggggcgc ctcgtccccg agcacgcgaa gccgcggccc gccggagaga
     2161 aacgctgacg cgatttcgga tttcggattg cggattgcgg attgaggaag cggggagggt
     2221 tgatgacagc gcggccccca tcaacactcc ccgctccttt aatccgcaat ccgaaatccg
     2281 caatccgcaa tcaccctccc gcccccgccc ctttgtctcc ccgtccctct gtgt
//
LOCUS       DBNGOH010000389         2540 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3725115, whole genome shotgun sequence.
ACCESSION   DBNGOH010000389 DBNGOH010000000
VERSION     DBNGOH010000389.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2540)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2540)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2540
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3725115"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1784
                     /locus_tag="AC159P_07750"
     CDS             <1..1784
                     /locus_tag="AC159P_07750"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020714359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004557 - alpha-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HIR3695384.1"
                     /translation="TEIRASEMRVVGTPRAEALRGDARASRLAERLGGRQLSVTLEDE
                     RGRVRATWRAVLSDGSNYVRQEVTLEALGADLPASEIRLVDVALVGARVEGTVTGSPV
                     VAGNLFLGFEHPLSVSRVEGARVTCSLPRELPLRAGTKVTYSSVIGVTRPGQLRRDFL
                     AYLERERAHPYRTFLHYNSWYDIGYTNKFDEKDSLGVINHYGRELVRERGVRMNSFLF
                     DDGWDDNSSLWSFHRGFPRGFTPLKEASARFGAAPGVWMSPWGGYDAAKAARLKYGEA
                     EGFEIRDDGGKFGPGFALSGPKYYARFREVCMQMIDRYGVNQFKIDGFGNANRAFPGS
                     RFDSDFDAAINLVREMRERRPDLFVNLTVGTFPSPFWLRYGDSIWRGGEDHDFAGVGT
                     DRQQWITYRDAETYKMTVRRGPLYPLNSLMLHGLVYAEKVSRSKRKKLDRDPHGDFDD
                     EIKSYFGSGTHLQELYVTPSLLKEKDWDLLAEAARWSRSNADVLVDTHWMGGDPARLE
                     VYGWASWSPRKGILVLRNPSDRAQEFTLDVGQAFELPAGAARAYTARSPWRDESRRAA
                     VRLSAGRPHTFRLAPFEVLTLEALPAR"
     gene            1844..>2540
                     /locus_tag="AC159P_07755"
     CDS             1844..>2540
                     /locus_tag="AC159P_07755"
                     /inference="COORDINATES: protein motif:HMM:NF016777.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR3695385.1"
                     /translation="MSKLNRRQWLASLAAASVLPALGRRGLAQQQGGQAAREGKLALE
                     EYVPKSMLHVPATKVARARFPAVDFHTHLSWTPADGRKANQNTTPAKALEVMNRRNVR
                     VMVNLTGGFGPALEENVRYWQQAYPERFVVFTEPWFSRFAEPNYPTFQAEQIEHARRV
                     GAKGIKVLKRLGLGLREQVTKGPLVKVDDKRFDPMWEAAGGARLPVAIHTSDPEAFFL
                     PTDKYNERYEELSA"
BASE COUNT          406 a          870 c          883 g          381 t
ORIGIN      
        1 gcacggagat tcgggcttcg gagatgcgtg tcgtcggcac gccccgcgcg gaggcgctgc
       61 gcggagacgc gcgcgcctcg cgcctcgcgg agcggctcgg cggcagacag ttgagcgtca
      121 cgctggaaga cgagcgcggg cgtgtgcgcg cgacgtggcg cgccgtgctc agcgacggct
      181 cgaactacgt ccggcaggag gtgacgctgg aggctctggg cgcagacctg cccgcctcgg
      241 agattcgcct cgtggatgtg gcgctcgtag gcgcgcgcgt cgaggggacg gtcacaggct
      301 cgcccgtcgt ggccggcaac cttttcctcg ggttcgagca cccgctctcg gtcagccgcg
      361 tcgagggcgc gcgcgtcacc tgctcgctcc cgcgcgagct gccgctgcgc gccgggacga
      421 aggtgactta ctcgtccgtc atcggcgtca cgcgtccagg gcagttgcgc cgcgacttcc
      481 tcgcctacct cgagcgcgag cgcgcgcacc cctaccgcac gttcctccac tacaactcct
      541 ggtacgacat cgggtacacg aacaagttcg acgagaagga ttcgctcggc gtcattaacc
      601 actacggccg cgagctggtg cgcgagcgcg gcgtgcggat gaactcgttc ctcttcgacg
      661 acggctggga cgacaactcc tcgctctgga gcttccatcg gggcttcccg cgcgggttca
      721 cgccgctcaa ggaggcgtcc gcccgcttcg gcgcggcgcc gggcgtgtgg atgtcgccgt
      781 ggggcggcta cgacgcggcg aaggcggcgc gcctcaagta cggcgaggcc gaagggttcg
      841 agatcaggga cgacggcggg aagttcggcc cgggcttcgc gctctcgggg ccgaagtact
      901 acgcgcgctt ccgcgaagtc tgcatgcaga tgatcgaccg ctacggcgtc aaccagttca
      961 agatcgacgg cttcggcaac gccaaccgcg ccttccccgg cagccgcttc gacagcgact
     1021 tcgacgccgc catcaacctt gtgcgcgaga tgcgcgagcg ccgccccgac ctcttcgtca
     1081 acctcacggt cggcaccttc ccttcgccct tctggctccg ctacggcgac tcgatctggc
     1141 gcggcggcga ggatcacgac ttcgccggcg tcggcacgga tcgccagcag tggatcacct
     1201 accgcgacgc cgagacctac aagatgacgg tgcggcgcgg gccgctctac cccttgaact
     1261 cgctgatgct ccacggcctc gtctacgccg agaaggtgtc gaggagcaaa cggaagaagc
     1321 tcgaccgcga cccgcacggc gacttcgacg acgagatcaa gtcatacttc ggctcgggca
     1381 cgcaccttca ggagctgtat gtcacgcctt cgctgctcaa ggagaaggat tgggacctgc
     1441 tcgccgaggc cgcgcgctgg tcgcgctcga acgccgacgt gctcgtggac acgcactgga
     1501 tggggggcga ccccgcgcgg ctcgaagtct acggctgggc ctcctggtcg ccgcgcaagg
     1561 gcatcctcgt cctgcgcaac ccttcggaca gggcgcagga gtttacgctc gacgtgggtc
     1621 aggctttcga gttgcccgcg ggggccgcgc gcgcctacac ggcccgcagc ccgtggcgcg
     1681 acgagtcgcg ccgcgccgcc gtcaggctga gcgccgggcg gccacacacg ttccggctcg
     1741 ccccgttcga ggtgctgacg ctcgaagccc tgccagcccg ttaggcgcga gaccgggccg
     1801 ttggtcggtt catgacattg ggcatccgag gtgagaagag accatgagta agctgaatcg
     1861 aaggcagtgg ctggcgagcc tcgccgccgc gtccgttcta ccggcgctcg ggcgcagagg
     1921 gctggcgcaa cagcagggcg ggcaggcggc aagggagggg aagctggcgc tcgaagagta
     1981 cgtgccgaag agcatgctgc acgtgccggc gacgaaggtg gcgcgcgcgc gcttcccggc
     2041 tgtggacttc cacacgcacc tctcttggac gccggccgac gggcggaagg ctaatcagaa
     2101 cacgacgccc gcgaaggcgc tcgaagtgat gaaccgccgc aacgtccgtg tgatggtcaa
     2161 cctcacgggc ggcttcggcc ccgccctcga agagaacgtg cgctactggc agcaggcgta
     2221 ccccgaacgc ttcgtcgtct tcaccgagcc gtggttcagc cggttcgcgg agccgaacta
     2281 cccgacgttc caggccgagc agatcgagca cgccaggcgc gtcggcgcga agggcatcaa
     2341 ggtgttgaag cggctcgggc tgggcctgcg cgagcaggtg acgaaggggc cgctcgtcaa
     2401 ggtcgatgac aagcgcttcg acccgatgtg ggaggcagcg ggcggcgcgc gcctccccgt
     2461 cgccatccac acctccgacc ccgaggcgtt cttcctcccg accgacaagt acaacgagcg
     2521 ctacgaggag ctgagcgcgc
//
LOCUS       DBNGOH010000390         3309 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1526025, whole genome shotgun sequence.
ACCESSION   DBNGOH010000390 DBNGOH010000000
VERSION     DBNGOH010000390.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3309)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3309)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3309
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1526025"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(31..1743)
                     /locus_tag="AC159P_07760"
     CDS             complement(31..1743)
                     /locus_tag="AC159P_07760"
                     /inference="COORDINATES: protein motif:HMM:NF025253.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HIR3695386.1"
                     /translation="MLKSAKGAGGTLNATSLTSITSAGDTTMTNVAGTGTSGRLAKWL
                     GSGGAGTLGDSAITESGGNVGVGTTAPGQKLEVAGNIKLSGAGSPLIFPDGSSMTTAS
                     GGGTPSGTSIVGAVNDPATSGVINDNRLAPTIARLGGANAWTGANVFGAGLSANNSLI
                     TNVGNPVNAGDAVNKAYTDANFVKFVPGAEQLSVGDANGTATMINLRGGSTCCSGPGG
                     HTPAFFKVFQNGSFVATGNLGIGTSPMEGKGYRTSWHTYKGAFRSGYADNEWDDANVG
                     FFSWAGGSNSTASGLYALAFGDTNFARSTSSIAFGSGNEVKGAAGFSAGAGHRVCDTY
                     CVALGNNARSGGPYINGKCDPETFNIRGLAAIAIGYNVTADQDHTTAMGKFASNNGFQ
                     GTFIWSDGAPQASADTFRNTANNESAARATGGFRFRTNLGGTTGCNLPAGSGVFNCTS
                     SRTTKENFTGVNGEDVLARLRKVPVSTWNYISEGGGARHLGPMAEDFYGAFQLGTSDK
                     AIGVQDAVGVSLAAVKALDARTLELQQKTEEVEQLRKQVNTLEQRLAALEAMMQKQGE
                     GQLR"
     gene            complement(1707..2336)
                     /locus_tag="AC159P_07765"
     CDS             complement(1707..2336)
                     /locus_tag="AC159P_07765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695387.1"
                     /translation="MRFTSPGQVLQMRLAVYSAEGALVFDSGLRKGSVLDWKPAEGSQ
                     PLADGAYMAVVTYKDFQGKLSQRVGALSVQSGQAALKRVRREELTAAQTQAPAASRQS
                     QKIEAADGFDSLTVVTEGRERAMTVATHDGQDGQVTSTSGAITFRTADIIAGADKEQV
                     RITPEGRVGIGTSKPEATLDVAGTVRARAAASSSRTARCSSRRRARAAR"
     gene            complement(2698..>3309)
                     /locus_tag="AC159P_07770"
     CDS             complement(2698..>3309)
                     /locus_tag="AC159P_07770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695388.1"
                     /translation="LAVLVEGDKVVVRSTQPRGVGKVILFERRGGAAGHSVGDAPAGE
                     TRLARPALAPAAAPPRDELKALLVAHGLYEREAEAMLNTWRDSWFEEGLRVFYVLPRE
                     AVDAILPLTIEPQPKELVRVLVGRAELITPEMERDVTAQLSKFEDPAPGAREAALREI
                     NKYGRFADTILGQLMRYAPDRQTQARVRRVLQATAPRPRQAIE"
BASE COUNT          429 a         1117 c         1189 g          574 t
ORIGIN      
        1 gcttcgcgcg tctggggagt cgggggagcc ttacctcagt tgaccctcgc cctgcttttg
       61 catcatcgct tcgagcgcgg cgaggcgctg ttcgagcgtg ttgacctgct tgcggagctg
      121 ctcgacctct tcggtcttct gctggagttc gagcgtccgc gcgtcgagcg ccttgacggc
      181 cgcgaggctc acccccaccg cgtcctgcac gccgatggcc ttgtcgctcg tgccgagctg
      241 gaaggcgccg tagaagtctt cggccatcgg gccgaggtgg cgggcgccgc cgccctcgga
      301 gatgtagttc cacgtcgaga ccggcacctt gcgcaagcga gcgagcacgt cctcgccgtt
      361 cacgcccgtg aagttctcct tcgtggtgcg gctcgaagtg cagttgaaga cgccggagcc
      421 tgcgggcagg ttgcagcccg tcgtgccgcc gaggttggtg cggaagcgga agccgcccgt
      481 ggcgcgcgcc gcggactcgt tgttggccgt gttgcggaac gtgtcggcgc tggcctgcgg
      541 cgcgccgtcc gaccagatga acgtgccctg gaagccgttg ttcgaggcga acttgcccat
      601 cgccgtcgtg tggtcctgat ccgccgtcac gttgtagccg atggcgatgg ccgcgaggcc
      661 gcggatgttg aaggtctctg ggtcgcactt gccgttgatg taggggccgc ccgagcgcgc
      721 gttgttgccg agtgcgacgc agtaggtgtc gcacacgcgg tggcccgcgc ccgcggagaa
      781 ccctgcggcg cccttcacct cgttgccgct gccgaaggcg atgctgctgg tgctgcgcgc
      841 gaagttggtg tcgccgaagg cgagcgcgta gaggccgctg gcggtcgagt tcgagccgcc
      901 cgcccacgag aagaagccga cgttggcgtc atcccattcg ttgtcggcgt agccggagcg
      961 gaacgcgccc ttgtaggtgt gccaggaggt gcggtagccc ttgccctcca tcggcgaggt
     1021 gccgatgccg aggttgccgg tggcgacgaa gctgccgttc tggaagacct tgaagaaggc
     1081 gggcgtgtgg ccgccggggc cggagcagca ggtcgagccg ccgcgcaggt tgatcatcgt
     1141 cgccgtgccg ttggcgtcgc ccaccgagag ctgctcggcg ccggggacga acttgacgaa
     1201 gttcgcgtcg gtataagcct tgttgacggc gtcgcccgcg ttgaccgggt tgccgacgtt
     1261 ggtgataagc gagttgttcg cggagaggcc cgcgccgaag acgttcgcgc ccgtccaagc
     1321 gttcgcgccg ccgagccgcg cgattgtcgg cgcgaggcgg ttgtcgttga tgacgccgga
     1381 ggtcgccggg tcgttgacgg cgccgacgat gctcgtgccc gagggcgtgc cgccgccgct
     1441 ggccgtcgtc atgctcgacc cgtcggggaa gatgagcggg ctgcccgcgc cgctgagctt
     1501 gatgttcccc gccacttcca gcttctggcc gggagccgtc gtgcccaccc cgacgttgcc
     1561 gccagactcc gtgatggccg agtcgccgag cgtgcccgcg ccgcccgacc ccagccactt
     1621 ggcgagcctg ccggaggtgc ccgtgcccgc gacgttggtc atcgtggtgt cgcccgcgct
     1681 ggtgatcgaa gtcagcgagg tcgcgttcaa cgtgccgccc gcgcccttcg ccgacttgag
     1741 caccgtgccg tcctcgaaga cgatgccgcc gcgcgcgcgc gcacggtgcc ggccacgtcg
     1801 agcgtcgcct cgggcttcga ggtgccgatg ccgacgcggc cctcgggcgt gatgcgcacc
     1861 tgctctttgt ctgcgcccgc gatgatgtcg gcggtgcgga aggtgatcgc gccgctggtg
     1921 ctcgtgacct gcccgtcctg cccgtcgtgg gtggcgacgg tcatggcccg ctccctgccc
     1981 tctgtgacga ccgtcagcga gtcgaagccg tcggcggcct cgatcttctg cgactgccgc
     2041 gaggcggcgg gcgcctgcgt ctgcgcggcg gtcagctcct cgcggcgcac gcgcttcaac
     2101 gccgcctgcc ccgactgcac ggagagcgcg ccgacccgct gcgagagctt gccctgaaaa
     2161 tccttgtacg tgacgacggc catgtacgcg ccgtcggcca acggctgcga gccttcggcg
     2221 ggcttccagt cgaggacgct gcccttgcgc aggccggagt cgaaaacgag cgcgccctcc
     2281 gcgctgtaga cggcgagccg catctgcaag acctgtccgg gggaagtgaa acgcacgccg
     2341 ccgcccgcgg ggaggcgagc acagccgtcg aggccgaggc cggcgaggcg tcctgacccg
     2401 cggcgttgcc ctgcgccgcg gtcgggacga cgaacattgt caatgcgagg aggcccggta
     2461 acagttgatt tgtgattaac gctttgccct tcattttacc ttccttgcgc caacggtttc
     2521 ccttcgtcca gctaacgcgc aaagtcgagg gcggtcggga tgaattattt tcggcccgcg
     2581 ggcgggaagg gtttttctgt agagaaaacg aacgctcgtt cggagaaggg cgcgcgccgc
     2641 gccttccgaa cgagcgtcga gagtgtctgc tgtgtacgaa cccgccgggg cgttggttca
     2701 ctcgatggcc tgacgcgggc gcggcgcggt cgcctgcaag acgcgccgga ctctggcctg
     2761 cgtctgcctg tcgggcgcgt acctcatgag ctgtccgagg atggtgtccg cgaagcgccc
     2821 gtacttgttg atctccctga gcgccgcctc gcgcgcgccc ggcgcggggt cttcgaactt
     2881 cgagagctgc gccgttacgt cgcgctccat ctcgggcgta atcaactccg cgcgcccgac
     2941 gagcacgcga accaactcct tcggctgcgg ctctatcgtg agcgggagga tcgcgtccac
     3001 ggcctcgcgc ggcaggacgt agaagacgcg caggccctct tcgaaccagg agtcgcgcca
     3061 agtgttgagc atcgcctcgg cctcgcgctc gtagaggccg tgcgcgacga gcagcgcttt
     3121 cagctcgtcg cgcggcgggg cggcggcggg cgcgagcgcg gggcgtgcga gccgcgtctc
     3181 gcccgcgggc gcgtcgccga ccgagtggcc cgcggcgccg ccccgccgct cgaagaggat
     3241 gaccttgccg accccgcgcg gctgcgtgct ccgcacgacc accttgtcgc cttcgacgag
     3301 gactgcgag
//
LOCUS       DBNGOH010000391         2005 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3726012, whole genome shotgun sequence.
ACCESSION   DBNGOH010000391 DBNGOH010000000
VERSION     DBNGOH010000391.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2005)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2005)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2005
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3726012"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1715)
                     /locus_tag="AC159P_07775"
     CDS             complement(<1..1715)
                     /locus_tag="AC159P_07775"
                     /inference="COORDINATES: protein motif:HMM:NF051698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HIR3695389.1"
                     /translation="MNRQTSLRGRVAAAALWALALALLAGPAALAQSGRSTVRGTVRD
                     QQGNVVTGATVTLTDAGRNFTRSQTTNDEGGYTFAAVPPGTYRVEVEAAGFKKAAFAD
                     VHAVVDTPVDVDAQLEAGLATETVTVTGSTEAPLNTTDASLGNAFESRRIQELPLNAR
                     NVVGLLSLQPGVTRGGYVNGGRADQANVTLDGVDVNEQQRGLDVVTDEAFASVLRVTP
                     DSLQEFRVTTTNPNADQGRSSGAQVSLVTKSGTNQLHGSLYHYHRNTVTTANDFFNNA
                     AGVERPQLLRNIFGGSLGGPIIKDRAFFFGTYEGFREATATSVLRQVPLPTLGQGIVR
                     YRTDSGASDPSCPAGTPAGVRCLNASQINAFYTASNGVTPGINPAALAALSSAASRYP
                     ANDPSVGDGLNTSGFRFNATTPGRLNTYITRFDLNLTDRQSLFLRGNYQWDHVTHSVY
                     GSPDCSGAAGDNIQCFPDTPPLTIWNHPWGIAVGHSWTISNTLVNRASYGFTRAAFTD
                     QGDQPGNFYSFRFIFRPLGFRGTLSRTTPVHNFVDDLSWIKGTHTFQFGGNVRLVTNN
                     RSSDAA"
BASE COUNT          297 a          607 c          780 g          321 t
ORIGIN      
        1 gcggcgtccg agctgcggtt gttggtgacg agccggacgt tgccgccgaa ctggaacgtg
       61 tgcgtgccct tgatccacga gaggtcgtcc acgaagttgt ggacgggggt cgtgcgcgag
      121 agcgtgcccc ggaagccgag cgggcggaag atgaagcgga acgagtagaa gttgccgggc
      181 tggtcgccct ggtcggtgaa ggcggcgcgc gtgaacccgt aggaggcgcg gttgacgagc
      241 gtgttcgaga tcgtccacga gtggccgacg gcgatgcccc acgggtggtt ccagatcgtg
      301 agcggcggcg tgtcggggaa gcactggatg ttgtcgcccg cggccccgct gcaatccggc
      361 gagccgtaga ccgagtgcgt gacgtgatcc cactggtagt tgccacgcag gaacaaagac
      421 tggcggtcgg tcaggttcag gtcgaagcgg gtgatgtagg tgttgagccg ccccggtgtg
      481 gtggcgttga agcggaagcc gctggtgttg aggccgtcgc ccacgctggg gtcgttcgcc
      541 gggtagcggc tggcggcgga ggagagcgcc gcgagcgccg ccgggttgat gccgggcgtc
      601 acgccgttgg aggcggtgta gaaggcgttg atctgtgagg cgttgaggca gcggacgccg
      661 gcgggcgtgc ccgccgggca cgaggggtcg ctcgcgccgc tgtcggtgcg gtagcgcacg
      721 atgccctggc cgagcgtcgg cagcggcacc tggcgcagca cggacgtggc cgtcgcctcg
      781 cggaagccct cgtaggtgcc gaagaagaag gcgcggtctt tgatgatggg gccgccgagt
      841 gagccgccga agatgttgcg caggagttgc gggcgctcga cgcccgccgc gttgttgaag
      901 aagtcgttgg cggtcgtcac cgtgttgcgg tggtagtggt agagcgagcc gtgcaactgg
      961 ttcgtgcccg acttggtgac gagcgagacc tgcgcgcccg aggagcggcc ctggtcggcg
     1021 ttcgggttgg tcgtcgtgac gcggaactcc tgtaacgagt cgggcgtcac gcgcaagacc
     1081 gaggcgaagg cttcgtcagt caccacgtcc agcccgcgct gctgctcgtt cacgtccacg
     1141 ccgtcgagcg tgacgttggc ctggtcggcg cgaccgccgt tgacgtagcc gccgcgcgtc
     1201 acgcccggct ggagcgagag caggccgacg acgttgcgcg cgttcaaggg caactcctga
     1261 atgcggcgcg actcgaaggc gttgccgagc gaggcgtcgg tcgtgttgag gggcgcctcg
     1321 gtgctgccgg tgacggtgac ggtctcggtc gcgaggccgg cttcgagttg cgcgtccacg
     1381 tccacgggcg tgtccacgac cgcgtgcacg tcggcgaacg ccgccttctt gaagcccgcg
     1441 gcctcgacct cgacgcggta ggtgcccggc ggcacggcgg cgaaggtgta gccgccttcg
     1501 tcgttggtcg tctgcgaccg cgtgaagttc cggcccgcgt cggtgagcgt cacggtggcg
     1561 cccgtgacga cgttgccctg ctggtcgcgc accgtgccgc gcacggtcga gcggccggac
     1621 tgcgcgagcg ccgccggccc ggccagcagc gcgagggcga gcgcccacag cgccgccgcc
     1681 gccacgcgtc ctctcagaga ggtctgtctg ttcatcgtgc ctccttatga tgggtgtagg
     1741 ccgcgcccgc ctgccgtaca ccgcactagc cgcgggcgcg ggaaccaggg gagtgtgaaa
     1801 ggaactgtcg cgggaacgcg gccaccggga aatgcgccga cgttcgcgcg tgcgtaagtt
     1861 aagtgcccga cctcatggcc gcccgtacga ggtgttgact aaagagtgac gcggaagatg
     1921 tgagctaact tgccaagacc gcaacgaagc gctgaccgcg tccggaaacg ctgacggggt
     1981 ggcccgccct cgacatcaaa tgaac
//
LOCUS       DBNGOH010000392         2894 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_5008594, whole genome shotgun sequence.
ACCESSION   DBNGOH010000392 DBNGOH010000000
VERSION     DBNGOH010000392.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2894)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2894)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2894
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_5008594"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            140..1456
                     /locus_tag="AC159P_07780"
     CDS             140..1456
                     /locus_tag="AC159P_07780"
                     /inference="COORDINATES: protein motif:HMM:NF014893.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HIR3695390.1"
                     /translation="MNLTEEQKTEGRRNFLKALAGTPALVALGAAATTVGPVSGGPVK
                     AGVVGVGGMGTELVARCQKEFIDVRAVCDINPRNRQKVAAAMVKAGWPEPRQYEEWRE
                     MLEKEDIEAVINATPLWAHADIVVGCLDAGKHVLSEKMMAKTEPDCRRMLEAARRNNK
                     VLEIGYQRYYNPMYQATYDNIVKAGVLGDVYHARLVWHRDRNWRRQQEPPTPDYNPSK
                     WGYPDWDHLLNWRVYKQYSEGLVAELGSHMLTVANWFFDSAPEAVYTTGGINRFKDRE
                     VFDHVYATFDYPGGRNVTFSSIESNGFEEAYEMFLGTKGTLVLKRETEAYLFPEKGAE
                     GGAAPAAQVTKVETSPQTTNPIADASATRPTNDPQGRTADASAATQQQTDRGLSYRDE
                     IAGFCAAVRTGQPVRCGPEKAMRSAVSVFAANQAADQKARVAVPKV"
     gene            1630..>2894
                     /locus_tag="AC159P_07785"
     CDS             1630..>2894
                     /locus_tag="AC159P_07785"
                     /inference="COORDINATES: protein motif:HMM:NF014893.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR3695391.1"
                     /translation="MSDSINFSRRTFVKAAGGAAAATIVSASPALASVTSQRRRYAIV
                     GTGGRAIGMWGRDLVQKYSDVLEFVGLCDINHKRVAVAKESIGAACPTFTNFDEMMDK
                     ARPDLLMVTTVDGFHRDYIVKGLERGADVMTEKPMVIDAGQCQDVLDAEKKVNKKIVV
                     TFNYRYAPKHQKIKEILMSGEIGNVTSVDFSWFLDVRHGADYFRRWHRLKSKGGSLWV
                     HKATHHFDLVNWWLAADPVEVVAHGDLNVYGRANDFRHTHCRPCPHKAKCPFYFDITK
                     VPSMMKLYVACEDVDNYHRDGCVFREDVDIYDTMSALVKYSNNVTMNYSLNAYLPIEG
                     YRLTFDGEKGRLEVRDYERQPWQVAEETEMYVTKSFGKREKITVQNAEGGHGGGDDRL
                     RDLIFRNAQAPEHMRLPDSRAGAMSCLTG"
BASE COUNT          576 a          970 c          914 g          434 t
ORIGIN      
        1 gtcgctgacg cgacgccgcg ttgctcgcaa cgactgttcc cggccttgaa aggccgggct
       61 aaactcagac gccgctaggc ggcggaaagg cgggcttcac gtttattcgt gcggaatgtt
      121 ttaaggaagg atcgttatca tgaatctcac ggaagagcag aagacggaag ggcggcgcaa
      181 ctttctgaag gcgctggcgg gcacgcccgc gctcgtcgct ctgggcgcgg cggcgacgac
      241 cgtggggccg gtcagcggcg gccccgtcaa ggcgggcgtg gtcggcgtcg gcggcatggg
      301 cacggagctg gtggcgcgct gtcagaagga gttcatcgac gtgcgcgccg tgtgcgacat
      361 caacccgcgc aaccggcaga aggtggcggc ggcgatggtc aaggccgggt ggcccgagcc
      421 gcggcagtac gaggagtggc gcgagatgtt ggagaaggaa gacatcgagg ccgtcatcaa
      481 cgccaccccc ttgtgggcgc acgccgacat cgtggtgggc tgtctcgacg cgggcaagca
      541 cgtcctctcc gagaagatga tggcgaagac cgagccggac tgccgccgca tgctcgaagc
      601 cgcgcggcgc aacaacaagg tgctggagat cggctaccag cgctactaca acccgatgta
      661 ccaggccacc tacgacaaca tcgtcaaggc gggcgtgctc ggcgacgtgt accacgcgcg
      721 gctcgtctgg caccgcgacc gcaactggcg ccgccagcag gagccgccca cgcccgacta
      781 caacccctcg aagtggggct acccggactg ggatcacctg ctgaactggc gcgtctacaa
      841 acagtattcc gaagggctgg tggccgaact cggcagccac atgctgacgg tcgccaactg
      901 gttcttcgac tcggcccccg aagcggtcta caccacgggc ggtatcaacc gcttcaaaga
      961 ccgcgaggtc ttcgaccacg tctacgcgac cttcgactat ccgggcgggc gcaacgtcac
     1021 cttctcgtcc atcgagtcga acggcttcga ggaggcctac gagatgttcc tcggcacgaa
     1081 gggcacgctc gtcctcaagc gcgaaacgga agcctatctc ttccccgaga agggagccga
     1141 gggcggcgcc gcgcccgccg cgcaggtcac gaaggtcgag acctcgccgc agacgacgaa
     1201 ccccatcgcc gacgcctcgg ccacgcgccc gaccaacgac ccgcaggggc gcaccgccga
     1261 cgcgagcgcc gcgacgcagc agcagaccga ccgcggcctc tcctaccgcg acgagatcgc
     1321 gggcttctgc gccgccgtcc gcacgggcca gcccgtccgc tgcggccccg aaaaggcgat
     1381 gcgctcggcg gtctccgtct tcgccgccaa ccaggccgca gaccagaagg cccgcgtggc
     1441 ggtaccgaag gtgtgaaaag aagcaggcgg taggcagtag gcggtaggca gttaaagcag
     1501 ttgaagaatt cgaaatccga gatttcgaat tcgaagcttg aattgccaaa cccaaatctg
     1561 aagtccgatg tttcttaact gcctaccgcc tactgccttc tgcctactat cttataagga
     1621 gtgatcccca tgtccgactc gatcaatttc agccgtcgca cgttcgtcaa ggccgcgggc
     1681 ggcgccgccg ccgcgaccat cgtctccgcc agtcccgcgc tggcctcggt cacttcgcag
     1741 cgccgccgct acgccatcgt cggcacgggg ggccgcgcca tcggcatgtg ggggcgcgac
     1801 ctcgtgcaga aatactcgga cgtgcttgag ttcgtcggcc tctgcgacat caaccacaag
     1861 cgcgtcgccg tggccaagga gtccatcggc gcggcctgcc cgacgttcac caacttcgac
     1921 gagatgatgg acaaggccag gcccgacctc ctgatggtga cgaccgtgga cggcttccac
     1981 cgcgactaca tcgtcaaggg gcttgagcgc ggggccgacg tgatgaccga aaagccgatg
     2041 gtcatcgacg ccggccagtg ccaggacgtg ctcgacgccg agaagaaggt gaacaagaag
     2101 atcgtcgtca ccttcaacta ccgctacgcg ccgaagcacc agaagatcaa agagattctg
     2161 atgtcgggcg agatcggcaa cgtcacctcg gtcgatttca gttggttcct cgacgtgcgc
     2221 cacggcgcgg actacttccg ccgctggcac cgcctgaagt ccaagggcgg gagcctgtgg
     2281 gtgcacaagg cgacgcacca cttcgacctc gtcaactggt ggctcgccgc agaccccgtg
     2341 gaagtcgtcg cccacggcga cctcaacgtc tacggcaggg ccaacgactt ccgccacaca
     2401 cactgccgcc cgtgcccgca caaggcgaag tgccccttct acttcgacat cacgaaggtg
     2461 ccttcgatga tgaagctcta cgtcgcgtgc gaggacgtgg acaactacca ccgcgacggc
     2521 tgcgtcttcc gcgaggacgt ggacatctac gacacgatgt cggcgctcgt caaatactcg
     2581 aacaacgtca cgatgaacta ctcgctcaac gcgtatctcc ccatcgaagg ctaccgcctg
     2641 accttcgacg gcgagaaggg ccgcctcgaa gtgcgcgact acgagcgcca gccgtggcag
     2701 gtcgccgaag agaccgagat gtacgtcacc aagagcttcg gcaagcgcga gaagatcacc
     2761 gtccagaacg ccgagggggg ccacggcggc ggcgacgacc gcctccgcga cctcatcttc
     2821 cgcaatgcac aggcgcccga acacatgcgc ctgcccgact cgcgcgccgg cgcgatgtcc
     2881 tgtctgacgg gcat
//
LOCUS       DBNGOH010000393         1595 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2809492, whole genome shotgun sequence.
ACCESSION   DBNGOH010000393 DBNGOH010000000
VERSION     DBNGOH010000393.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1595)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1595)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1595
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2809492"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..351)
                     /locus_tag="AC159P_07790"
     CDS             complement(<1..351)
                     /locus_tag="AC159P_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010481307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695392.1"
                     /translation="MAIRKMHDRGGAEERRESRALVIGGSLAGLLAARVLADHFDRVT
                     VVERDTYPETAEARKGVPQARHVHALLRRGQMILEQLFPGLRAEMMAAGAPVVDTGGD
                     MAWLSPAGWGVRFDS"
     gene            complement(456..746)
                     /locus_tag="AC159P_07795"
     CDS             complement(456..746)
                     /locus_tag="AC159P_07795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695393.1"
                     /translation="MSTQMGTAAARDRLIVIEGGGWRSLAAWCEQCGARAGMIRSDDF
                     ASASHVSPQAVERLTEAGAFHGTVDAEGVPFICMNSPLKIVPRADIALAAAH"
     gene            933..>1595
                     /locus_tag="AC159P_07800"
     CDS             933..>1595
                     /locus_tag="AC159P_07800"
                     /inference="COORDINATES: protein motif:HMM:NF012698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HIR3695394.1"
                     /translation="MNSNPGGRAYLFGPFVVDTREGLLLREGQPVPLTPKAFETLVAL
                     VENSGHCLGKDELMRRVWPDSFVEENNLSQNISQLRRALQAEGADSPPYIETVPRRGY
                     RFCAHVDVREGALIIRERTRTRIVIEEEDDDGEGVGASSGRAGAAAAVGAVDEGAVRD
                     EFPPELMRVPETKYALSGDVNIAYQTLGDAPLDLVFVMGWVSHLEYFWREPRFARFLR
                     RLA"
BASE COUNT          227 a          587 c          531 g          250 t
ORIGIN      
        1 ggagtcgaag cgcacgcccc agcccgcggg cgacagccac gccatgtcgc cccccgtgtc
       61 caccaccggc gcgcccgccg ccatcatctc ggcccgcagg ccggggaaga gctgctccag
      121 gatcatctgc ccgcgccgca gcagcgcgtg cacgtgcctg gcctgcggca cgcccttgcg
      181 cgcctcggcg gtctccgggt acgtgtcgcg ctcgacgacc gtcacgcggt cgaagtgatc
      241 cgcgagcacg cgcgccgcca gcagcccggc gaggctcccg ccgatgacga gcgcccggct
      301 ctcccgccgc tcttcggcac cgcccctgtc atgcatcttt cgtatcgcca taagacttcg
      361 gaatgcctcc ttagtgctct tgaaattccg ttcctgaagt gacgcgcgtt tccgcgcgtc
      421 cgcgtcgcgt gcgccccgcg cgggaggctg gccggttagt gcgcggcggc cagcgcgatg
      481 tccgccctcg gcacgatctt cagcggcgag ttcatgcaga tgaacgggac gccttccgcg
      541 tccacggtgc cgtggaaggc cccggcttcc gtcaggcgct cgaccgcctg cgggctgacg
      601 tggctcgcgg aggcgaagtc gtccgaacgg atcatccccg cccgcgcgcc gcactgctcg
      661 caccacgcgg ccaggctccg ccacccgccg ccctcgatca cgatcaggcg gtctcgcgcc
      721 gccgccgtcc ccatttgcgt agacatcttt cgtcggcctc ctctgactgt tcctcacgcc
      781 cacgtcttcg agtagaatgg agcgagactt tccgctcccg ccgcccgacc tcgaacacgg
      841 cgcggagctt acccgccgcg ggcgcgcggg gtctttagcg gcttctgaaa tttttctgaa
      901 agagttgtga gggcggacgg aggcgacgac cgatgaactc gaaccccggc gggcgggcct
      961 accttttcgg ccccttcgtc gtggacaccc gcgaagggct gctgctgcgc gaggggcagc
     1021 ccgtgccgct cacgccgaag gcgttcgaga cgctggtcgc gctggtcgag aacagcggcc
     1081 actgtctcgg caaggacgag ctgatgcggc gcgtctggcc cgacagcttc gtcgaggaga
     1141 acaacctctc gcagaacatc tcgcaactgc gccgcgcctt gcaggcagag ggcgcagact
     1201 cgccgcccta catcgagacc gtgccgcgcc gcggctatcg cttctgcgcg cacgtcgatg
     1261 tgcgcgaggg cgccctcatc atccgcgaga ggacgcggac gcggatcgtc atcgaagagg
     1321 aggacgacga cggcgaaggt gtcggcgcgt cgtcaggcag ggcgggcgcc gccgccgccg
     1381 tcggggctgt ggacgagggc gccgtgaggg acgagttccc gccggagctg atgcgcgtcc
     1441 ccgagacgaa gtacgcgctg agcggcgacg tgaacatcgc ctaccaaacg ctcggcgacg
     1501 cgccgctcga cctcgtcttc gtgatgggct gggtctcgca cctcgaatac ttctggcgcg
     1561 agccgcgctt cgcgcgcttc ctgcggcggc tcgcc
//
LOCUS       DBNGOH010000394         3910 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2809578, whole genome shotgun sequence.
ACCESSION   DBNGOH010000394 DBNGOH010000000
VERSION     DBNGOH010000394.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3910)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3910)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3910
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2809578"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1066)
                     /locus_tag="AC159P_07805"
     CDS             complement(<1..1066)
                     /locus_tag="AC159P_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020471021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HIR3695395.1"
                     /translation="MPDRSPLDDTDAQTASAPPRGPEPWRGYVVAVAAVAAATLLMFA
                     LYAVLGIRRGQVPFIFYFGAVLLAARHGGRGPGLLSVFLSALAAYYFFVPPFNEMALD
                     REGVLQTTVFVLVASMIVMLAEASRRAERATQAGERSLRITLRSIGDAVIATDASGRI
                     TFMNPVAERLTGWQPAEARGRLLAEVFRIVNEETRAEVESPVVKVLRDGAVVGLANHT
                     VLISKAGAETPIDDSGAPIKDEAGRIIGVVLVFHDITERRRAEGALARLAAIVESSDD
                     AIIGKTLDGRITNWNAAAERLYGYAAEEVVGRHISLLVPAELAGELEDILRRLRAGQP
                     VEHLETVRLRKDGTRINVSIT"
     gene            1572..2243
                     /locus_tag="AC159P_07810"
     CDS             1572..2243
                     /locus_tag="AC159P_07810"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012680666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR3695396.1"
                     /translation="MYPAGNLFIDAPHGRLEAILKEPRAEGGAGPRGAALVLHPHPLH
                     GGTMHNKVVFRTARALNDAGVVSLRVNFRGVGHSTGVHTGARGGEQEDARVALDYLVG
                     QYPNLPLALAGFSFGARVGLEVGTYDERVRWLAGVGTPVSIEERDYDFSFLRDCRKPL
                     LLVHGERDEFGTVEDLRALAAQAPPEARVRVEVIHGAGHFFDEQLEDLRRVVAGWAEE
                     RLREG"
     gene            2341..>3910
                     /locus_tag="AC159P_07815"
     CDS             2341..>3910
                     /locus_tag="AC159P_07815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695397.1"
                     /translation="MENINGEAAPAAESAAAAERAAPTARAAAVVPAAAARALARERL
                     FVLLFGALVIGVAFWSLQFATASVCCGDFDAYYHFRWSRMLLDGALAGQFPPSFDALP
                     LTTLNARDYVDHHFLFHVFQIPFTLFGDMQRGAKLGTWLFACLAVFALHWLLVRYRVS
                     YPLIWLVLILGSAAPFLYRIHMGKAMSVSIVLLVAGIHLLFERRYAWLLPLAFVFALT
                     YDMFLLLWVAAGVWTAVVLWGERRVEWRPLAFVVAGSLLGYVVNPYFPHNLQLIYQHV
                     MMKVTAKDFSTAVGGEWYPYNTLEFIANCGPAFAAMIVGYVAFRDSTRREQQRPLFFL
                     LFATFLMLVNMRWRRFSEYWPPFAIVFAAFALQPFIERMRARYTSVPAANDDRATDEP
                     AAPAAENATGGWPSSRDEHEGAAVGIVCGAALYLLALYAVPGVFTGRAAAFGGFLLGG
                     AALALVLLYAWPRGWTRALIMAFALAAFGVMGWYHRITVGDIAGMPGPDQYRAGMQWI
                     TANVPPGEPVYNADW"
BASE COUNT          529 a         1364 c         1372 g          645 t
ORIGIN      
        1 cggtgatcga gacgttgatg cgcgtgccgt ccttgcgcag gcgcacggtc tcaaggtgct
       61 cgaccggctg gccggcgcgc aggcggcgga ggatgtcttc cagctcgccc gcgagttccg
      121 cggggacgag cagggagatg tggcgaccga cgacctcttc ggcggcgtag ccgtagaggc
      181 gctcggcggc ggcgttccag ttggtgatac ggccgtcgag cgtcttgccg atgatggcgt
      241 cgtccgacga ttcgacgatg gcggcgagcc gcgcgagcgc gccctcggcc cgcctgcgtt
      301 cggtgatgtc gtggaagacg agcacgacgc cgatgatccg gccggcctcg tctttgatgg
      361 gggcgccgct gtcgtctatc ggcgtctcgg cgcccgcctt cgagatgagc accgtgtggt
      421 tcgccagccc gacgacggcg ccgtcgcgca gcaccttcac gacggggctt tcgacctcgg
      481 cgcgcgtctc ctcgttgacg atgcggaaga cctccgcgag gagccgcccg cgcgcctccg
      541 ccggctgcca gccggtcagc cgctcggcca cggggttcat gaaggtgatg cggcccgagg
      601 cgtccgtggc gatgacggcg tcgccgatgc tgcggagcgt gatccgcagc gaccgctcgc
      661 cggcctgcgt cgcgcgctcg gcgcggcgac tcgcctccgc cagcatgacg atcatggacg
      721 cgaccaggac gaagaccgtg gtctgcaaca cgccctcgcg gtcgagcgcc atctcgttga
      781 acggggggac gaagaagtag taggccgcga gcgcggagag gaagaccgag agcagccccg
      841 gcccgcgacc gccgtggcgc gcggcgagga gcacggcgcc gaagtagaag atgaagggaa
      901 cctggccgcg ccggatgccg agcacggcgt agagcgcgaa catgaggagc gtggccgccg
      961 cgacagccgc gaccgcgacg acgtagccgc gccacggctc cggcccgcgc ggcggggccg
     1021 aggccgtctg cgcgtcggtg tcgtcgagag ggctgcggtc aggcatcgat catcctctgc
     1081 tctgttcgtg aagcgcgtgc gtcctcgaag cccgccgacg gcgcggggcc gcccgccgcc
     1141 cgtccgcatc gcggggcgcc ctcgcgcgcc cgcgcccgtc ggaacatccg ggccgcgtcc
     1201 gtcgccgtcg tcgcggtaag ctgtagatcg agatcgaaga agctcaaaga gtgtcagccg
     1261 cgtcgcgcgt gggggccggg ccgccccggc agtcgtgact ccccacgctc aaaaatcttc
     1321 tgcctgtcaa tgcggccttg aggttacatt caagctactg ctttcaaaaa cacaagtcaa
     1381 gacctttacc gtcgcgcccc gccgcgcagc cgttagccac cggggaacat ttctggtaaa
     1441 aacagtaggc cgtaggcggt aggcagcagg cagttatcga gcgcggacgg tgttgacgct
     1501 tgcaccctgt ccctgcgaca cagagaaggt ttttactgcc tacggcctac ggcctaccgc
     1561 ctactgttcc tatgtatcct gccggcaacc tcttcatcga cgcgccgcac gggcggctgg
     1621 aggcgatcct gaaggagccg cgcgcggagg gcggcgccgg gccgcgcggc gcggcgctcg
     1681 tgctgcaccc gcaccctctg cacggcggga cgatgcacaa caaggtggtc ttccgcacgg
     1741 cgcgcgcgct gaacgacgca ggcgtggtca gcctgcgcgt caacttccgc ggcgtcggcc
     1801 actcgacggg cgtgcacacg ggcgcgcggg gcggcgagca ggaggacgcg cgcgtcgcac
     1861 tcgattatct ggtcgggcag tacccgaacc tgccgttggc gctcgcgggc ttctcgttcg
     1921 gcgcgcgcgt cgggcttgag gtcgggacgt acgacgagcg cgtgcgctgg ctcgccggcg
     1981 tcggcacgcc cgtcagcatc gaggagcgcg actacgactt ctccttcctg cgcgactgcc
     2041 gcaagccgct gctcctcgtc cacggcgagc gcgacgagtt cggcacggtc gaagacctgc
     2101 gcgcgctcgc cgcgcaggcg ccacccgagg cgcgcgtccg cgtcgaggtc atccacggcg
     2161 ccggacactt cttcgacgag cagctcgaag acctgcgccg cgtcgtcgcc gggtgggcgg
     2221 aagagcggtt gcgcgaaggc tgagcaggga gcgggcgtgg acggttgggc gccggggcgg
     2281 atacttttcc gaaggctcgg tcttcacacg cggatacttt tccgaagggt tagtcggcac
     2341 atggagaaca tcaacggcga ggcggcgccc gccgcagagt ctgcggcggc ggcggagcgc
     2401 gcggccccta cggcgcgggc ggcggcggtc gtcccggcgg cggcggcgcg cgcgctcgcg
     2461 agagagcggc tgttcgtgct cctcttcggc gcgctcgtca tcggcgtcgc gttctggtct
     2521 ttgcagttcg ccacggcctc tgtctgctgc ggcgacttcg acgcgtacta ccacttccgc
     2581 tggagccgga tgctccttga cggcgcgctc gccggacagt tcccgccctc gttcgacgcg
     2641 ctgccgctga cgacgctgaa cgcgcgcgac tacgtcgatc accacttcct cttccacgtc
     2701 ttccagatac cgttcacgct cttcggcgac atgcagcgcg gcgcgaagct ggggacgtgg
     2761 ctcttcgcct gcctcgccgt cttcgcgctc cactggctgc tcgtccgcta ccgcgtgagc
     2821 tacccgctca tctggctcgt cctcatcttg gggagcgcgg cgcccttcct ctaccgcatc
     2881 cacatgggca aggcgatgag cgtctccatc gtgctgctcg tcgcgggaat ccatcttctg
     2941 ttcgagcgca ggtacgcgtg gctgctgccg ctcgcgttcg tcttcgcgct gacgtacgac
     3001 atgttcctgc tgctgtgggt ggccgcaggg gtgtggacgg cggtggtgct gtggggcgag
     3061 cggcgcgtcg agtggcgccc gctcgcgttc gtcgtcgccg gttcgctcct cggctacgtc
     3121 gtcaacccct acttcccgca caacctgcaa ctcatctacc agcacgtgat gatgaaggtg
     3181 acggcgaagg acttttcgac ggcggtcggc ggcgagtggt atccgtataa cactctggag
     3241 ttcatcgcca actgcgggcc ggccttcgcc gcgatgatcg tcggctacgt cgccttccgc
     3301 gactcgacgc ggcgcgagca gcagcgcccg ctcttcttcc tcctcttcgc caccttcctc
     3361 atgctcgtca acatgcgctg gcggcgcttc tccgaatact ggccgccctt cgccatcgtc
     3421 ttcgccgcct tcgcgctgca acccttcatc gaacgcatgc gcgcgcgcta cacgtcggtg
     3481 cccgccgcga acgacgaccg cgcgacggac gaacccgcgg cgcccgccgc cgagaacgcg
     3541 acgggcggct ggccctcttc gcgcgacgag cacgagggcg ccgcggtcgg catcgtctgc
     3601 ggcgcggcgc tctacctgct cgcgctgtac gcggttccgg gcgtcttcac ggggcgcgcg
     3661 gcggcgttcg gcgggttcct gctggggggc gcggcgttgg ccctcgtgct gctctacgcg
     3721 tggccgcgcg gctggacgcg ggcgctcatc atggccttcg cgctcgccgc cttcggcgtg
     3781 atgggctggt atcaccgcat caccgtgggc gacatcgcgg ggatgcccgg ccccgaccag
     3841 taccgcgccg ggatgcagtg gatcacggcc aacgtgccgc cgggcgagcc ggtctacaac
     3901 gccgactggg
//
LOCUS       DBNGOH010000395         3594 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4642063, whole genome shotgun sequence.
ACCESSION   DBNGOH010000395 DBNGOH010000000
VERSION     DBNGOH010000395.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3594)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3594)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3594
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4642063"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..126)
                     /locus_tag="AC159P_07820"
     CDS             complement(<1..126)
                     /locus_tag="AC159P_07820"
                     /inference="COORDINATES: protein motif:HMM:NF024891.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR3695398.1"
                     /translation="MATLYVASTETFVGKSATCVGLLDRARRDGFTAGYMKPVSVS"
     gene            complement(203..1504)
                     /locus_tag="AC159P_07825"
     CDS             complement(203..1504)
                     /locus_tag="AC159P_07825"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3695399.1"
                     /translation="MAVALRVENVSKQYRIYGRPGDRLTESLTRGRLKRHREFWALKE
                     VSFEVEAGTTVGVVGPNGCGKSTLLQIIAGTLEPTHGGVWREGRVAALLELGAGFDPE
                     FTGVENVYMNAALMGLTRRETDRLFPDIERFAEIGDFLRQPVKTYSSGMYVRLAFAIA
                     ASVEPDILVIDEALAVGDAVFQHRCLRRIKEIQERGSTVLFVSHDAAAVRALCSRAIL
                     LSAGRVLAEGKPADVLNRYQKIIMEREEAYDEAARAARDGEERHAGEDEDETLAAPRP
                     AFRHGDGSAEIVGAELLDATRRAAAVVETGESVSVRVRARFHAAVESPVIGFLIRNRH
                     GIHVYGTNTKEQQLEFGAVRRGETIEVTFAFDCWLGIDQHSVSLAVHSRDGISYDWMD
                     GVIFFRVTSAVLVEGVANLNATATARRLETRSAVAEAVKRF"
     gene            1625..2458
                     /locus_tag="AC159P_07830"
     CDS             1625..2458
                     /locus_tag="AC159P_07830"
                     /inference="COORDINATES: protein motif:HMM:NF013245.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3695400.1"
                     /translation="MRGMISAQTARRAVWRPLWELPRRFDLIWPLARRMFVARYRGSA
                     LGLVWAVLTPLALIAVFTFLFAGVFGARFTSEGSTWDYALYLFCGLLPWTAFQESVQQ
                     SSGVVVAHANLVKRVVFPLETLPVAQALAALLGQLCGTAALVVAALVIRGELHATALW
                     LPVLLAPQLLLTLGASWLVASLGVFLRDTAQALALVLMVWMYLTPVIYPEAVVPVRYH
                     ALLALNPFTHLVRSYRNCLLEGAPPDWPGLAYFALVALAAFVLGYWWFARTRKNFADV
                     I"
     gene            2696..3526
                     /locus_tag="AC159P_07835"
     CDS             2696..3526
                     /locus_tag="AC159P_07835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695401.1"
                     /translation="MVAKTAQEYLSQLGIQLSFGKSRQKEGGFSNFDEERMLARFVAE
                     LLPAEHSRTAVDIGAGDGVKASNTYALFRAGWRGLGVEAHRRRARRLARNLKNFAGVA
                     ASSQLVTPENVVALLRAHDIPRDFSVLSIDIDSYDYWVLDALLKSYRPYLVVTEINEK
                     IPPPIRFVVKYDPDFQPAHHFFGHSIQSLADLCDAHGYRIIALEYNNAFLAPAELPGL
                     RPLTAEDAYRTGYLERPDRHEKFHRNHDMEILHTLSPADGVKYIDQYFAAQRAKYELR
                     "
BASE COUNT          548 a         1249 c         1193 g          604 t
ORIGIN      
        1 cgagacgctg accggcttca tatagcccgc cgtgaagcca tcccgccgcg cccgatccag
       61 caggccgacg caggtggcgc tcttgccgac gaaggtctcg gtcgaggcga catagagggt
      121 agccatagct ctcttccttc attttggatt gtggattttg gattttggat tgtcacgaaa
      181 tccaaaatcc acaatccaca atctaaaatc gtttcaccgc ttcggccacc gcgctgcgcg
      241 tttccagccg ccgcgcggtg gcggtcgcgt tgaggttggc gacgccttcg acgaggacgg
      301 cgctcgtgac gcggaagaag atgacgccgt ccatccagtc gtagctgatg ccgtcgcggc
      361 tgtggacggc gagggagacg gaatgctggt cgatgccgag ccagcagtcg aaggcgaaag
      421 tgacttcgat ggtctctccg cgccgcaccg cgccgaactc cagttgctgc tccttcgtgt
      481 tcgtgccgta gacgtggatg ccgtggcggt tgcggatgag aaagccgatg accggactct
      541 ccactgccgc gtggaagcgg gcgcgcacgc gcaccgacac gctctcgccc gtctcgacga
      601 ccgccgccgc gcgccgcgtc gcgtccagca gctcggcgcc gacgatctcg gcgctcccgt
      661 cgccgtggcg gaaggcgggg cgcggcgcgg cgagcgtctc gtcttcgtct tcgccggcgt
      721 gtcgctcttc gccgtcgcgc gcggcgcggg cggcctcgtc gtacgcctcc tcgcgctcca
      781 tgatgatctt ctggtagcgg ttgagcacgt ccgcgggctt gccttcggcg aggacgcggc
      841 ccgcgcttaa aagtatcgcg cgcgagcaga gggcgcgcac cgccgccgcg tcgtgcgaga
      901 cgaagaggac ggtcgagccg cgttcctgaa tctccttgat gcggcgcagg cagcggtgct
      961 ggaagaccgc gtcgccgacg gccagcgcct cgtcgatgac gaggatgtcc ggctcgacgc
     1021 tggcggcgat ggcgaaggcg agccgcacgt acatccccga agagtaggtc ttgacgggct
     1081 ggcgcaggaa gtcgccgatc tccgcgaagc gctcgatgtc ggggaagagc cggtcggtct
     1141 cgcggcgcgt gaggcccatc agcgcggcgt tcatgtagac gttctcgacg cctgtgaact
     1201 ccgggtcgaa gcccgcgccg agttccagca gcgcggccac gcgcccctcg cgccagacgc
     1261 cgccgtgcgt cggctccaga gtgcccgcga tgatctgcaa gagcgtggac ttgccgcagc
     1321 cgttcggccc gacgacgccg acggtcgtcc ccgcctcgac ctcgaagctc acctccttga
     1381 gcgcccagaa ctcgcggtgc cgcttgagcc gcccgcgggt cagagactcc gtcaggcggt
     1441 cgcccggtcg cccgtaaatc cggtactgct tcgagacgtt ttcgacgcgc agggccacgg
     1501 ccatgcgccg caatgtaaca gaggtcggaa gcgcaaagcc agaagccggg gaggcgcgcg
     1561 gcggactcga agaggcccgc gaggcccgcg ccgcaaggct tgctctctcc cgactcctgt
     1621 tacaatgcgc ggcatgatct cggcgcagac ggcgcggcgc gcggtgtggc gaccgctgtg
     1681 ggagttgccg cggcggttcg atctgatctg gccgctggcg cggcggatgt tcgtggcgcg
     1741 ctaccgcggg tcggcgctcg ggctggtgtg ggcggtgctg acgccgctgg cgctgatcgc
     1801 ggtcttcacg ttcctcttcg cgggcgtctt cggagcgcgg ttcacgagcg aaggctcgac
     1861 ctgggactac gcgctctacc tgttctgcgg gctgctgccg tggacggcgt tccaggagtc
     1921 ggtgcagcag tcttcgggcg tggtcgtcgc gcacgcgaac ctcgtcaagc gcgtcgtctt
     1981 ccctttggag acgctgccgg tggcgcaggc gctggcggcg ctcctggggc agttgtgcgg
     2041 gacggcggcg ctcgtcgtgg cggcgctcgt catccgcggc gagctgcacg cgacggcgct
     2101 gtggctgccg gtgctgctcg cgccgcagct tctgctgacg ctcggcgcgt cgtggctggt
     2161 cgcttcgctc ggcgttttcc tgcgcgacac ggcgcaggcg ctcgcgctcg tgctgatggt
     2221 gtggatgtac ctgacgccgg tcatctaccc tgaggcggtc gtgcccgtgc gctaccacgc
     2281 gctgctcgcc ctgaacccct tcacccacct tgtccgcagc taccgcaact gcctgctcga
     2341 aggcgcgccg cccgactggc cgggcctcgc ctacttcgcg ctcgtcgcgc tggccgcctt
     2401 cgtcctcggc tactggtggt tcgcgcgcac gcgcaagaac ttcgcggacg tgatctgagc
     2461 gggttcacgc gaagggctgc gcgtgtgggc gggttgacga gagtcgccgt cgtctggcat
     2521 aaaaaacaac ctgttcgagc ggcggcggca cggacgcgag acgccgtccc gctcggtccc
     2581 aaactgccgc acaagggttc gaagcttcag cgcaggccgg cggcgccgcc cccgttcaca
     2641 cacccgttcg ccgtggaggg tgctcgacgc cgcgcccgcg cgcatcgcgg tatctatggt
     2701 tgcgaagacg gcacaggagt acctcagcca gctcggcatc cagttgagct tcggcaagtc
     2761 gcgccagaag gagggcggct tctcgaactt cgacgaggag cggatgctgg cgcggttcgt
     2821 cgcggagctg ctgcccgccg aacacagccg caccgccgtg gacatcggcg cgggcgacgg
     2881 cgtgaaggct tcgaacacct acgcgctgtt ccgcgcgggc tggcgcggcc tcggcgtcga
     2941 ggcccaccgc cgccgcgcgc gccgtctcgc acgcaacctg aaaaacttcg cgggtgtcgc
     3001 ggccagcagc cagctcgtca cgccggagaa cgtcgtcgcg ctcctccgcg cgcacgacat
     3061 cccgcgcgac ttcagcgtgc tgagcatcga catcgacagc tacgactact gggtgctcga
     3121 cgcgctgctc aagtcctacc gcccctacct cgtcgtcacc gagatcaacg agaagatacc
     3181 gccgcccatc cgcttcgtcg tcaagtacga ccccgatttt cagcccgcgc accacttctt
     3241 cggccacagc atccagagcc tcgcagacct ctgcgacgcg cacggctacc ggatcatcgc
     3301 cctcgaatac aacaacgcct tcctcgcgcc cgcggagctg cccggcttgc gcccgctcac
     3361 cgccgaggac gcctaccgca caggctacct cgaacgcccc gaccgccacg agaaattcca
     3421 ccgcaaccac gacatggaaa tcctccacac gctcagcccc gccgacggcg tcaaatacat
     3481 cgaccaatac ttcgccgccc agcgcgccaa atacgaactg agatagagcc gtgaaccgtg
     3541 aaccgtcaac cgtgacaaga gaggacagat tttatcttgt cacggttgac ggtt
//
LOCUS       DBNGOH010000396         3981 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_977644, whole genome shotgun sequence.
ACCESSION   DBNGOH010000396 DBNGOH010000000
VERSION     DBNGOH010000396.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3981)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3981)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3981
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_977644"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..442)
                     /locus_tag="AC159P_07840"
     CDS             complement(<1..442)
                     /locus_tag="AC159P_07840"
                     /inference="COORDINATES: protein motif:HMM:NF019317.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695402.1"
                     /translation="MSNKRHGRRPSFISPIGEMRLSFFRFLARRFNWFRPTTRFLVGF
                     LTLALLTTLLLARTRSLMPAAESYREGDIVSADVIAPADITAEDVGETERRREAARRR
                     TPSVWNHDPTVAERAAQTFRASWSSLKQQADARDDNINNNAGANV"
     gene            complement(448..2286)
                     /gene="hflX"
                     /locus_tag="AC159P_07845"
     CDS             complement(448..2286)
                     /gene="hflX"
                     /locus_tag="AC159P_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012470945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HIR3695403.1"
                     /translation="MSAAPRRRPAARRAFACARGCTEGTPEGLDISNVHGNTQGLKPN
                     QLRRIEKLYSRRIAPQQVVTPEFARQMSELSHETRRQLGALIDRKGYVEYVVVGDARR
                     IELPDLKRTRVSEGRFRGLRCVHTHLRGEELTQDDLNDLALLRLDLMVAINVDERTGL
                     PGRVRAAHLLPTTAEEVDDGEHAVPYGFLEAPVPSQLDVDFLELINSLEEELARVRLS
                     TRRAALRDRAILVGVTTGSVEDSEESMAELRELAASAGVVVLNEIIQRRQAIDPRTVL
                     GKGKLDELIVRALRLGADVIVFDRELSPAQVRSVSEATDLKVIDRAQLILDIFAQRAQ
                     TRDGKIQVELAQLKYMLPRLIAGQDSAFSRLAGGIGGRGPGETKLETDRRRVRDRINR
                     LEKEIEAQRMRRRQRRKERTRRELPVISIVGYTNAGKSTLLNTLTSSTVLAEQRMFAT
                     LDPTSRRLRLPREQEVIINDTVGFIRDLPPDLLAAFRATLEEIDESDLLIHLVDASNP
                     RWQQQVESVERILGDLGHADLPLLVVFNKADLVAPDDLDAILRQASLDGARECFAVSA
                     LSAASLRPVIERAGEILARDLAAEHERNVSPAADDPPDAEAAAFTR"
     gene            2672..3703
                     /locus_tag="AC159P_07850"
     CDS             2672..3703
                     /locus_tag="AC159P_07850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Vgb family protein"
                     /protein_id="HIR3695404.1"
                     /translation="MSKGRIERVDPAAAIPGGEVAVECTDYDTSNLRACRALFDGAEA
                     RMVGAGPRRVLAIVPETSEGGAGVEVVLESGDAQRTDSARLAVGRKLAEDLHLVANPA
                     YDPDDGSLYVTRSGTRGQRMPVSILRIDTRGELHSLHGEVANPTGMAFDSTGQMFVTS
                     RMDGTVYRLTPFHEVVAFARNLGVATGLAFDRYDRMYVGDRTGTIHRVNGVGEADAWA
                     LLEPSVAAYHMAFGPDGGLYVTGPTVSSFDSVTRVDEDGRTSVFYRGLGRPQGLAFDR
                     DGNLYVAASLRGRRGVVRISPDGRRAELVVAGMNVVGLAFSAEGDMAVATNEAVYSLP
                     LGIKGTLLK"
     gene            3782..>3981
                     /locus_tag="AC159P_07855"
     CDS             3782..>3981
                     /locus_tag="AC159P_07855"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011135248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HIR3695405.1"
                     /translation="MESELIGRLIIYIVVLLMAISAHEAAHAWMSHKFGDDTAYLLGR
                     VTLNPVAHTDPIGTLLIPIVGF"
BASE COUNT          599 a         1233 c         1479 g          670 t
ORIGIN      
        1 tgacgttcgc gccggcgttg ttgttgatgt tgtcgtcgcg ggcgtccgct tgttgtttga
       61 gcgaagacca ggaggcgcga aaagtttgtg cggcgcgctc ggcgacggtc gggtcatggt
      121 tccagacgga gggcgtgcgg cggcgcgcgg cctcacggcg gcgctcggtc tcgcccacgt
      181 cttcggcggt gatgtcggcg ggcgcgatca cgtcggcgct gacgatgtcg ccctcgcggt
      241 agctctcggc ggcgggcatc agcgagcggg tgcgcgcgag gagcagcgtc gtgaggagcg
      301 cgagcgtgag gaagccgacg aggaagcgcg tggtcgggcg gaaccagttg aagcggcgcg
      361 cgaggaagcg gaagaagctg agccgcatct cgccgatggg tgagatgaaa ctggggcgcc
      421 gcccgtgtct cttgttgctc atggggttta ccgtgtgaag gccgcggcct cggcgtcggg
      481 cgggtcgtcc gccgccggag agacgttgcg ttcgtgttcg gcggcgaggt cgcgcgcgag
      541 gatttcgccc gcgcgttcga tgacgggccg gagcgaggcg gcgctaagcg cggagacggc
      601 gaagcactcg cgcgcgccgt ccagcgatgc ctgacggagg atggcgtcga ggtcgtcggg
      661 cgcgacgagg tcggccttgt tgaagacgac gagcagcggc aaatccgcgt ggccgaggtc
      721 gccgaggatg cgctcgaccg actcgacctg ctgctgccag cgcgggttcg aggcgtcaac
      781 cagatggatg agcaggtcgc tttcgtcgat ctcttcgagc gtcgcgcgga aggcggccag
      841 caggtcgggc ggcaggtcgc ggatgaagcc gaccgtgtcg ttgatgatga cctcctgctc
      901 gcgcggcagg cgcaggcggc gcgaggtcgg gtcgagcgtg gcgaacatcc gttgctcggc
      961 gagcacggtg ctcgaagtga gcgtgttcag cagcgtcgat ttgcccgcgt tcgtgtagcc
     1021 gacgatggag atgaccggca gctcgcggcg cgtgcgctcc ttgcgccgct gccggcggcg
     1081 catgcgctgg gcctcgatct ccttttcgag gcggttgatg cggtcgcgca cgcggcggcg
     1141 gtcggtctcc agcttcgtct cgccggggcc gcgcccgccg atgccgcccg ccagccgcga
     1201 gaaggccgag tcctgaccgg cgatgagtcg cgggagcatg tacttgagct gcgccagctc
     1261 gacctgaatc ttgccgtcgc gcgtctgcgc gcgttgcgcg aagatgtcga ggatgagctg
     1321 cgcgcggtcg atgactttga ggtcggtcgc ctcggagacg gagcgcacct gcgccggcga
     1381 cagctcgcgg tcgaagacga tcacgtccgc cccgagccgg agcgcgcgca cgatgagttc
     1441 gtcgagcttg cctttgccga gcacggtgcg cgggtcgatc gcctgccggc gctggatgat
     1501 ctcgttgagg acgacgacgc ccgccgaggc ggccagctcg cgcagctcgg ccatcgactc
     1561 ttcggagtcc tcgaccgaac ccgtggtgac gccgacgagg atggcgcggt cgcgcagggc
     1621 cgcgcggcgc gtggagaggc ggacgcgcgc cagctcctct tcgagcgagt tgatgagttc
     1681 gagaaagtcc acgtcgagct gcgaggggac aggcgcttcc aagaatccgt atggcacggc
     1741 gtgctcgccg tcgtcgacct cttcggcggt cgtcggcagc aggtgtgcgg cgcgcaccct
     1801 gccgggcagt cccgtgcgct cgtcaacgtt gatggcgacc atcagatcga gacgcagcag
     1861 cgcgaggtcg ttgaggtcgt cctgcgtcag ctcctcgccg cgcaggtgcg tgtggacgca
     1921 gcgcaggccg cggaagcgcc cctcggagac gcgggtgcgc ttgaggtcgg gcagctcgat
     1981 gcggcgggcg tcgccgacga cgacgtattc gacgtagccc ttgcggtcga tgagcgcgcc
     2041 gagctgccga cgggtctcgt gcgagagttc cgacatctgc cgcgcgaact cgggcgtgac
     2101 gacctgctgc ggcgcgatgc ggcgcgagta gagcttctcg atgcggcgga gctggttcgg
     2161 cttcagcccc tgtgtgttcc cgtgtacgtt gctgatgtcg agtccctccg gcgtcccctc
     2221 cgtgcatccc cgcgcgcacg caaacgcgcg ccgcgccgct ggtcggcggc gcggcgcggc
     2281 gctcaacgtc tcaagttaga aatagagtga agcgagcggt tggagtcgga cgctgttcag
     2341 atactggagt ctgcgttgtc gctctttgcg cgagcactac gtttgatcgc ttcgggccgg
     2401 gcgccgccgc tcgtccggct ctgccgggag ggcgcgtttt gttaataaat ccgtgcgcga
     2461 gtatagcaca gcgcgcgtgg acgcaagcac aaaaagcggg ccgcacttgc actcgataga
     2521 cagcccgcgc gagaccgtcg tatgatgacg gcaccgttcg cgtagagaca aaaacagttc
     2581 gagaattggc atttcaggtt tgaaatgctg agttgtcgaa ttcgaatttt aaagcgcggc
     2641 ggcggcgcaa actttggggc ggggaggccg gatgagtaag ggacggatcg agcgtgttga
     2701 ccccgcggcg gcgatccccg gcggcgaggt cgccgtcgag tgcacggact acgacacgag
     2761 caacctgcgc gcgtgccgcg cgctgttcga cggcgcggag gcgcggatgg tgggcgccgg
     2821 cccgcggcgc gtgctggcca tcgtgccgga gacgagcgag ggcggcgcgg gcgtcgaggt
     2881 cgtgctcgaa agcggcgacg cgcagcgcac ggactccgcg cgcctcgcgg tgggccgcaa
     2941 gctcgcggaa gatttgcacc tcgtcgccaa ccccgcctac gacccggacg acggctcgct
     3001 ctacgtcacg cgctccggca cgcgcgggca gcggatgccg gtctcgattt taagaatcga
     3061 cacgcgcggc gagctgcaca gcctgcacgg cgaggtcgcc aaccccacgg gcatggcctt
     3121 cgacagcacg gggcagatgt tcgtcacaag ccgcatggac ggcacggtct accggctgac
     3181 gccgttccac gaggtcgtcg ccttcgcgcg caatttgggc gtcgcgacag ggctggcctt
     3241 cgaccgctac gaccgcatgt acgtgggcga ccgcacgggc acgatccacc gcgtcaacgg
     3301 cgtcggcgag gcggacgcgt gggcgctcct ggagccttcg gtcgcggcct accacatggc
     3361 gttcgggccg gacggggggc tgtacgtgac ggggccgacc gtgtcgagct tcgactcggt
     3421 gacccgcgtg gacgaggacg ggcgcaccag tgttttttat cgcggcctcg ggcgcccgca
     3481 ggggttagcc ttcgaccgcg acggcaacct ctacgtcgcg gcgtcgctca ggggtcggcg
     3541 cggcgtcgtc cgcatctcgc ccgacgggcg gcgcgccgaa ctcgtcgtcg ccgggatgaa
     3601 cgtcgtcggc ctcgccttca gcgcggaggg cgacatggcc gtcgccacca acgaggcggt
     3661 ctacagcctg cccctcggga taaaagggac actcttgaag taagcaggcg attgctcttt
     3721 aaaaaccgcc gtgctacttt gctgacttcg tgcgggcgcg gtctgacggg acaagatggg
     3781 aatggaatcg gaattaatcg gtcggctgat tatttatatc gtcgtgttgc tgatggcgat
     3841 ctcggcgcac gaggcggcgc atgcgtggat gtcgcataaa ttcggagacg acacggcgta
     3901 cctgctcggt cgcgttacgc tcaaccccgt cgcgcacaca gacccgatcg gcaccttgct
     3961 gatcccgatc gtgggcttca t
//
LOCUS       DBNGOH010000397         7794 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1344901, whole genome shotgun sequence.
ACCESSION   DBNGOH010000397 DBNGOH010000000
VERSION     DBNGOH010000397.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7794)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7794)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7794
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1344901"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /locus_tag="AC159P_07860"
     CDS             complement(<1..257)
                     /locus_tag="AC159P_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695406.1"
                     /translation="MIVLTFILIPLPFVVGCKEDPTQKATVAPQEQALTASASPGATP
                     SPTPTPLARPSGPIEFTDVTNEAGIRFRHNSGAAGKKYLPET"
     gene            complement(338..1210)
                     /locus_tag="AC159P_07865"
     CDS             complement(338..1210)
                     /locus_tag="AC159P_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695407.1"
                     /translation="MSATTVNTSSPVATPKAEAKQPKLFGIDSRFVAPIFITFILLVG
                     QLTYGILESYAMTVLAILTAIALELVLGRVYTGRWPHLASAYISGISVGILTRSPFAW
                     PFALCSAISIASKYVLRVRGRHIWNPSNFGICLMLLVAPWAYSTLSVQWGNNLWPVAI
                     IWALGLVITYHVGRIHITLTYVVSFFALAFVRSLLTGHTFLAEAAPITGPMYQLFIFF
                     MITDPKTTVRSSKLAQGAVAFAVALVETILRLFENVHAPYYALFIAGPLANLIEIYLT
                     RSKVPPTPSVEAAT"
     gene            complement(1401..3203)
                     /locus_tag="AC159P_07870"
     CDS             complement(1401..3203)
                     /locus_tag="AC159P_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016482673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HIR3695408.1"
                     /translation="MPKALVKKILLLLFFAALLATPFVLRRWHGLKDGNDTNAAAERE
                     SALKRYGFHLEEVSKQVGIDFTHRAPRLDAKLEHIMPQVASMGAAVSVVDFDRDGWQD
                     LYVTNSGEGSKNALFRNNADGGFADVAESMGVADVNRAETGVSMGAVWGDYDNDGFED
                     LFLYKWGRPELFHNDGGKSFTRVTERAGLPAWVNANTALWFDYDRDGRLDLFLGGYYP
                     EDVNLWRLTTTKIMPESFEYARNGGRKYLLRNRGDGAFEDMTASVGIDTRRWALAASA
                     ADLRGTGYPDLFIANDYGVAELFFNEEGKRFRDAGEQTGVGFAPKSGMNVAFGDILNR
                     GSLAVYVTNISEEGVLIQGNNLWVPREGASGDRLKYDEMAREMGVQLGGWSFGAQFGD
                     LNNDGALDLYLTNGYVSLERGTNYWYDFTKISGGNSTIIADAANWPPMRNRSLSGFQP
                     KRVWVNDGAGKFTDVAQTVGARDTFDGRAVVLVDLWNRGALDVVVANQRGPLLVYKNT
                     VAPDNVWVAFELEGAESNRSAVGAQVRVRWAGQEQVQEVSGGTGFCAQNQRRLHFGLG
                     RGARVERVEIRWPSGKVQTLEAPEVGRVHRVREA"
     gene            complement(3235..6810)
                     /locus_tag="AC159P_07875"
     CDS             complement(3235..6810)
                     /locus_tag="AC159P_07875"
                     /inference="COORDINATES: protein motif:HMM:NF024907.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HIR3695409.1"
                     /translation="MSPNQLQGAPLSVRACGRFFNDQRRGASLVLLFALAASGLVACR
                     RADVPDTTSPDYARAVRAFYVGLSALQVGDDARAEARLKDATRLAPDEPAAWANLGLL
                     YLRQREAERAAEHLEKARRLAPESAHVHVLFGLLESSRGRYSESVAALRRAVELDPKD
                     LKALYALASEVEREGGETGEEETVRLLRRILEAAPANVAVELDLARLAAKRGDAETLR
                     AIVARLAAKSASWPPEAQEQFKLLQAASSAENVRAAAPRVQFLRNVLARVPEYRQSLA
                     AVKLPAEIVGEPLTKPLRLAAPSPRPAPPDEGLTFAVEVLPGFAERDWAWARAVSLDA
                     ESAPAIMVGRGVPISLRDSPPDKIPGAVYIAGGQRLDYPERPSGDSLAGPRDQRVAAL
                     DFDYDFKVDLALAGEGFRLYRQEGAGKFADVTARTKLPAEVLSADYGGAWAADVEADG
                     DLDLALARHKPDALVVLQNNGDGTFTQTSPFGQQGRPAADFEWGDMDGDGDSDAAVRY
                     VGGGLAFFSNERGGLFRERALPPDLGMVSAVALADLDGDGRLDLLAARHDGSVLRVSD
                     RAEGTAWETSVVSPAFEGGLSGVNPPAVIVADFDNNGALDLLLAVQAAPFGARIMLGD
                     GRGSFKPLASAPEMSVDSAADLDGDGLLDLVGTDISGKAVRALGRGTKNYHWQTIRPR
                     AQEATGDQRINSFGVGGEIEVRAGLLTQKQPITGPSVHFGLGTETQTDVARINWPNGV
                     LQAEFELKGDTAALAEQRLKGSCPWVFAFDGERVSFVTDTLWRSPLGLKINAQDTAGV
                     MMTEEWIKIRADQLRPREGFYDVRITAELWETHFFDHVSLMVVDHPADTEVFIDERFA
                     VPPPALKVHATARPRAFAAATDDGGQDVSSIVAAQDGKYLDNFGRGRYQGITREHFVE
                     LELPADAPREGPLWLVGAGWIHPTDSSINVAVSQGAQPKPQGLSLEVADDSGGWTTAL
                     PALGFPAGKNKTVLIDISNVFRPNAPRRLRLKTNLEIFWDKLAWAEGLPATELKTERL
                     MPGEAELRYRGYSAVAQADKSSPELPDYNRLAGTVAVWRDLVGYHTRFGDVRELLKTV
                     DDRYVILNAGDEMLFKFAAPAPPAVGWSRDFVLVCDGWEKDGDYNTTFSKTVLPLPTH
                     ASADYTKAPTRLEDDPVYRKHQQDWQDYHTRYVTPRAMGDALRQR"
     gene            complement(7088..7717)
                     /locus_tag="AC159P_07880"
     CDS             complement(7088..7717)
                     /locus_tag="AC159P_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3695410.1"
                     /translation="MSGGQIKIMVVDDQAVVRQGFVSLINTVPDMVVVAEGTNGQQAV
                     ELYREHQPDVVLMDLRMPVMGGVEAIKAIKREFPAARLVVLTTFDGDEDIYRSLQAGA
                     QGYLLKDMFFEELEDAIRTVRAGGRRIPGVVAERLAERVGGSNLTGRELEVLEQIVRG
                     KSNKEIAAVFRISEATVKSHINSILSKLGVADRTQAATTALQRGIVHLP"
BASE COUNT         1226 a         2572 c         2675 g         1321 t
ORIGIN      
        1 gtctcgggca ggtacttctt gcccgccgcg ccgctgttgt ggcggaagcg gatgcccgcc
       61 tcgttcgtga catcggtgaa ctcgatcggc cccgaggggc gcgcgagcgg cgtcggcgtc
      121 gggctgggcg tcgcgccggg cgaggccgag gcggtcaacg cctgctcctg cggcgcgacg
      181 gtcgccttct gcgtcgggtc ttccttgcag ccgacgacga aaggtaaagg gatgagaatg
      241 aatgtcagaa cgatcaagcg acagcgcatg ttcgaatctc gccttgaaac ttgaatgtgg
      301 agacttgcgt ttcgggttgc ccgcgccttc gggtcgttca cgtcgccgct tcgacggaag
      361 gggtcggggg cactttactt ctcgtcaggt aaatctcgat caggttcgcc agcgggccgg
      421 cgatgaagag cgcgtagtag ggcgcgtgga cgttctcgaa gaggcggagg atcgtctcga
      481 ccagcgcgac ggcgaaggcg acggcgcctt gcgccagttt ggaagagcgg acggtcgtct
      541 tcgggtcggt aatcatgaag aagatgaaga gctggtacat cggcccggtg atgggcgcgg
      601 cctcggcgag gaaggtgtgg ccggtcagca ggctccgcac gaaggcgagc gcgaagaacg
      661 agacgacgta ggtcagcgtg atgtggatgc gcccgacgtg gtacgtgatg acgaggccga
      721 gcgcccagat gatggccacg ggccagaggt tgttgcccca ctgcacgctc aaggtcgagt
      781 aggcccacgg cgcgacgagc agcatcaggc agatgccgaa gttcgaaggg ttccagatgt
      841 ggcggccccg cacgcgcagg acgtacttcg aggcgatcga gatggcgctg cacagcgcga
      901 agggccacgc gaagggcgag cgcgtgagga tgccgacgct gatgcccgag atgtaggcgc
      961 tcgccagatg cggccagcgg cccgtgtaga cgcgcccgag cacgagttcg agcgcgatgg
     1021 ccgtcaagat ggccagcacc gtcatcgcgt acgattcgag gatgccgtag gtgagctggc
     1081 cgacgagcag gatgaaggtg atgaagatcg gcgcgacgaa gcggctgtcg atgccgaaga
     1141 gcttgggctg cttcgcctcg gccttcggcg tggcgacagg cgacgatgtg ttgactgtcg
     1201 tggcgctcat ccttataact cccgagttgt cgttgactag tcttcggatt caacacagag
     1261 aacacaaagg attcacagag ggcacggaga agacccgttt cgattttcga gcttcttttt
     1321 cttctccgtg ctctctgtgt ttcctctgtg ccctctgtgt tgaaatgaac tgacccttac
     1381 actcaaccag cgcgtcaggc tcacgcctct ctcacgcgat gcaccctgcc gacctccggc
     1441 gcttcgaggg tctggacttt gcccgacggc cagcgtatct cgacgcgctc gacgcgcgcg
     1501 ccgcggccca ggccgaagtg gaggcggcgc tggttctgcg cgcagaagcc ggtgccgccc
     1561 gacacctcct gcacctgctc ctgaccggcc cagcggacgc gcacctgcgc gccgacggcg
     1621 ctgcggttgc tctcggcgcc ttccagctcg aacgcgaccc agacgttgtc gggcgcgacc
     1681 gtgttcttat acaccagcag cgggccgcgc tggttggcga ccaccacgtc gagcgcgccg
     1741 cggttccaca gatcgacgag cacgaccgcg cgcccgtcga acgtgtcgcg cgcgcccacc
     1801 gtctgcgcca cgtccgtgaa cttgcccgcg ccgtcgttga cccagacgcg cttgggctgg
     1861 aagcccgaga ggctgcggtt ccgcatcggc ggccagttgg cggcgtcggc gatgatggtc
     1921 gagttgccgc ccgaaatctt cgtgaagtcg taccaatagt tcgtgccgcg ctccagagag
     1981 acgtagccgt tggttaaata cagatcgagc gcgccgtcgt tgttgaggtc gccgaactgc
     2041 gcgccgaagc tccacccgcc gagctggacg cccatctcgc gcgccatctc gtcatacttc
     2101 aaccggtcgc cgctcgcgcc ctcgcgcggc acccacaggt tgttaccctg gatgagcacg
     2161 ccctcttcgg agatgttggt gacgtagacg gcgaggctgc cccggttgag gatatcgccg
     2221 aaggcgacgt tcatcccgct cttcggcgcg aagccgacgc ccgtctgctc gcccgcgtcg
     2281 cggaagcgct tgccctcttc gttgaagaac agctcggcga cgccgtagtc gttggcaatg
     2341 aagaggtccg ggtagcctgt gccgcgcagg tctgccgccg aggcggcgag cgcccagcgg
     2401 cgcgtgtcga tgccgacgct cgcggtcatg tcctcgaacg cgccgtcgcc gcggttgcgc
     2461 agcaggtact tacgcccgcc attgcgcgcg tactcgaagc tctccggcat gatcttcgtc
     2521 gtcgtcagcc gccagaggtt cacgtcctcc gggtagtagc cgccgaggaa gaggtcgagc
     2581 cgcccgtcgc ggtcgtagtc gaaccacagt gccgtgttgg cgttgaccca cgcgggaagg
     2641 ccggcgcgct cggtgacgcg cgtaaaactt tttccgccgt cgttatggaa cagctcgggc
     2701 cgcccccact tgtagaggaa gaggtcttcg aagccgtcgt tgtcgtaatc gccccacacc
     2761 gcgcccatcg agacgcccgt ctcggcgcgg ttcacgtccg ccacgcccat gctttcggcc
     2821 acgtccgcga aaccgccgtc cgcgttgttg cggaagagcg cgttcttact tccttcgccg
     2881 ctgttcgtca cgtagaggtc ttgccagccg tcgcggtcga agtccacgac cgacacggcg
     2941 gcgcccatcg aagcgacctg cggcatgatg tgttcgagct tcgcgtcgag ccgcggcgca
     3001 cggtgcgtga agtcgatccc gacctgcttc gacacctctt cgagatggaa gccgtaacgc
     3061 ttgagcgccg actcgcgctc cgccgcagcg ttcgtgtcgt tgccgtcctt cagcccgtgc
     3121 cagcggcgca gcacgaaggg cgtcgccagc agcgcggcga agaagaggag gagaaggatt
     3181 tttttgacca aggctttcgg catcgtcgga acatcaagcg cccgtcgtca tcgttcacct
     3241 ttgccgcagc gcgtcgccca tcgcgcgcgg cgtgacgtag cgcgtgtggt agtcctgcca
     3301 gtcctgctgg tgcttgcggt agaccgggtc gtcttcgagg cgcgtcgggg ccttagtgta
     3361 atcggcgctc gcgtgcgtcg gcaaaggcag gacggtcttg gagaaggtcg tgttgtagtc
     3421 gccgtccttc tcccagccgt cgcacacgag cacgaagtcg cgcgaccagc ccacggcggg
     3481 cggggcgggc gccgcgaact tgaagagcat ctcgtcgccc gcgttcagaa tcacgtagcg
     3541 gtcgtccacg gtcttgagca gctcgcgcac gtcgccgaag cgcgtgtgat agccgacgag
     3601 gtcgcgccag acggcgacag tccccgcgag gcggttgtag tcgggcaact ccggcgaaga
     3661 cttgtcggcc tgcgcgaccg cggagtagcc gcggtaacga agctccgcct cgccgggcat
     3721 gagccgctcg gtcttcagct cggtcgcggg cagcccttcg gcccacgcga gtttgtccca
     3781 gaagatttcg aggttcgtct tcagacgcag gcggcgcggc gcgttcgggc ggaagacgtt
     3841 cgagatgtcg atcagcaccg tcttgttctt gcccgcgggg aagccgagcg caggaagggc
     3901 cgtcgtccag ccgccgctgt cgtcggcgac ttcgaggctg aggccctgcg gcttcggctg
     3961 cgcgccctgg ctgacggcga cattgatcga gctgtcggtc gggtgaatcc agcccgcgcc
     4021 gacaagccac aaaggcccct cgcgcggcgc gtccgcaggc agctcaagct cgacgaagtg
     4081 ctcgcgcgtg atgccctgat agcgaccgcg accgaagtta tcgaggtact tgccgtcctg
     4141 cgcggcgacg atggaagaaa cgtcctggcc accgtcgtcc gtcgcggcgg cgaaggctcg
     4201 gggccgcgcg gtcgcgtgga ctttgagcgc gggcggcggg acggcgaagc gctcgtcgat
     4261 gaagacctcc gtgtccgcgg ggtgatcgac gaccatcagc gagacgtggt cgaagaagtg
     4321 cgtctcccac agctcggcgg tgatgcgcac gtcgtagaag ccttcgcgcg ggcgcagctg
     4381 gtcggcgcga atcttgatcc actcttcggt catcatcacg cccgccgtgt cctgcgcgtt
     4441 gatcttcaga ccgaggggcg agcgccagag cgtgtcggtg acgaacgaga cgcgctctcc
     4501 gtcgaaggcg aagacccacg ggcaggagcc tttcaggcgc tgctcggcga gcgccgccgt
     4561 gtcgcccttc agctcgaact cggcttgcag cacgccgttc ggccagttga tgcgggccac
     4621 gtcggtctgc gtctccgtgc cgaggccgaa gtggacggac ggccccgtga tcggctgctt
     4681 ctgcgtgagc agaccggcgc gcacctcgat ctcgccgccg acgccgaacg agttgatgcg
     4741 ctggtcgccg gtcgcctcct gcgcgcgcgg tcgaatcgtc tgccagtggt aattcttcgt
     4801 gccgcgacct aaagcgcgca cggccttgcc actaatgtcc gtcccgacaa ggtcgagcag
     4861 accgtcgccg tcaagatcgg cagccgagtc caccgacatc tccggcgcgg acgcgagcgg
     4921 cttgaaagaa ccgcgtccgt cgccgagcat gatgcgtgcc ccgaacggag ccgcctgtac
     4981 ggcgagaagg aggtcgaggg cgccgttgtt gtcgaagtct gcgacgatga cggcgggcgg
     5041 gttgactccc gagaggccgc cctcgaacgc gggcgagacg acggacgtct cccatgcggt
     5101 gccctccgcc ctgtccgaga cgcggagcac tgaaccgtcg tggcgcgcgg ccagcaggtc
     5161 gagccgcccg tcgccgtcaa gatcggctaa ggcgacggcg gagaccatgc cgaggtcggg
     5221 cgggagcgcg cgctcgcgga aaagaccgcc gcgctcgttc gagaagaagg cgagcccccc
     5281 gcccacgtaa cgcactgcgg cgtcggaatc gccgtcgccg tccatgtcgc cccattcgaa
     5341 gtcggcggcg ggccgtccct gctgcccgaa cggggaggtc tgcgtgaacg ttccgtcgcc
     5401 gttgttctgc aagaccacca gcgcgtcggg cttgtgccgc gcgagcgcca gatcgaggtc
     5461 gccgtcggcc tccacgtcgg cggcccacgc cccgccgtag tccgccgaga ggacttcggc
     5521 gggtagtttc gtgcgcgccg tcacgtccgc gaacttcccg gcgccctcct gcctgtagag
     5581 tcggaagcct tcgcccgcca gcgccagatc gaccttgaag tcgtaatcga aatccaacgc
     5641 cgccacgcgc tggtcgcgcg ggccagccag agaatccccg gacggcctct cggggtaatc
     5701 gagccgctgc ccgccggcga tgtacacggc ccccgggatt ttatccgggg gactgtcccg
     5761 cagagatatc ggcacacccc gcccgaccat gatcgccggc gcgctctcgg cgtcgagcga
     5821 gacggcgcgt gcccatgccc agtctctttc cgcgaagccg ggcaggactt cgacagcgaa
     5881 cgtcaatccc tcgtcgggcg gcgcgggtcg cggagaaggc gccgcgaggc ggagcggctt
     5941 cgtcagcggc tcgcccacaa tctcggcggg gagtttgacg gcggcgaggc tctgtctgta
     6001 ttcggggacg cgcgcgagca cgttgcgcag gaattgcacg cgcggcgcgg cggcgcgcac
     6061 gttctcggcg gagctggccg cctgcaagag cttgaactgt tcctgcgctt cgggaggcca
     6121 cgacgcggac ttcgcggcga gacgagctac gattgcgcgg agcgtctcgg cgtcgccgcg
     6181 cttggccgcg agccgcgcga ggtcgagttc gacggcgacg ttcgcgggcg ccgcttcgag
     6241 gattcttcgc agcagccgga cggtctcctc ctcgcccgtc tcgccgccct cgcgctcgac
     6301 ttcggaggcg agcgcgtaga gcgccttcaa atctttcggg tcgagttcga cggcgcggcg
     6361 caaggcggcg accgattccg agtacctacc gcggctgctt tcgagcagcc cgaagaggac
     6421 gtgcacgtgc gcgctttcgg gcgcgagccg gcgcgccttt tcgaggtgct cggcggcgcg
     6481 ctcggcctcg cgctgcctga ggtagagcag gccgaggttg gcccacgccg caggctcgtc
     6541 gggcgcgagg cgcgtcgcgt ccttgagccg cgcctcggcg cgcgcgtcgt cgccgacctg
     6601 aagcgcggag aggccgacgt agaaggcgcg caccgcccgc gcgtagtccg gggaagtcgt
     6661 gtcgggaacg tccgcgcgcc ggcaggcgac gaggcccgac gccgcgagcg cgaagaggag
     6721 gacgagggaa gcgccgcgcc gttggtcgtt gaagaagcgc ccgcacgctc tgacggaaag
     6781 cggcgccccc tgaagctggt tgggactcac ctgttgaacc ttacgggcgc gggccgcggc
     6841 ggctgcgtca gcgcggcgcg cgtgagacgg gaaaacccga ccccgacttc gaatgatccg
     6901 cggtgactca caggttgctg gtgttcggac agcaacaaat tcttggagtg agttgacctg
     6961 tgattgatct cgtccggggc cgggttgaat tcaaccgagc gcccgcacag taacacggcc
     7021 cgccccgcct gtcatgaaac cgtgggctgg acgcccggtc atactttagt tggagacggc
     7081 gacggcctca cggcaggtgg acgatgccgc gttggagcgc ggtggtggcg gcctgcgtgc
     7141 ggtcggccac gccgagcttg ctgaggatgc tgttgatgtg gctcttgacg gtcgcctcgc
     7201 ttatccggaa gacggcggcg atctccttgt tgctcttgcc gcgcacgatc tgttcgagca
     7261 cttccagctc cctgcccgtc aggttcgagc cgccgacgcg ctcggcgagc ctttcggcga
     7321 cgacgccggg gatgcgccgg ccccccgcgc gaacggtgcg gatggcgtcc tccagctcct
     7381 cgaagaacat gtccttgagc agataccctt gcgcgcccgc ttgcagcgag cggtagatgt
     7441 cctcgtcgcc gtcgaacgtc gtcagcacga cgagccgcgc cgccgggaac tcgcgcttga
     7501 tcgccttgat ggcctcgacg ccgcccatca cgggcatccg caaatccatc agcaccacgt
     7561 ccggctggtg ctcgcggtac agctcgacgg cctgctggcc gttcgtcccc tctgcgacga
     7621 cgaccatgtc cggcacggtg ttgatcagag agacgaagcc ctgccgaacg accgcctggt
     7681 cgtccaccac catgattttt atctggccgc cgctcatgtc aaaagtcctc tccggcagtg
     7741 tgcccgcgca acgactcagg cgttcgccta actctcaagc gggacgccga ccgt
//
LOCUS       DBNGOH010000398         5363 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4460298, whole genome shotgun sequence.
ACCESSION   DBNGOH010000398 DBNGOH010000000
VERSION     DBNGOH010000398.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5363)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5363)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5363
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4460298"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(2..1315)
                     /locus_tag="AC159P_07885"
     CDS             complement(2..1315)
                     /locus_tag="AC159P_07885"
                     /inference="COORDINATES: protein motif:HMM:NF024808.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR3695411.1"
                     /translation="MRQRPNCLPNIIAVLLVVALALAPLGGCAGSDSGGGAKELSLAV
                     NSGVEGDALKAAARDYEAQTGVRVRIAEFPYANLFEKELVDLQAATGAYDLIMLDDPW
                     VPRFATRDFLTDLAPLFAKKNLPGPDADFVPTSLALCREPYETGTLYALPYVGNSQLF
                     FYRRDLFEKHQLEPPATWDDVLAAARAIHEREAAGSPSGGRVHGYVMRAAQGNAVVAD
                     FMPIFWAFGAEMFDAEGRPTVNSAEGVAALRFMLELGKYAPPGYASFNADEVSAHLLQ
                     GTAAMSINWPAWIGAFADTARSQVTGRMEFTTMPGARNAGRAEIGNWLVAIPGASRNA
                     EAAFDFLLWATSAAEMKRSALRGNPPTRRAVFTDAELVAKFPSYPAQLRSLETSRPRP
                     RTPLWNEIENAFGVYLSKANGGELSPEEAMTQANAKIAEIVARGR"
     gene            1449..2267
                     /locus_tag="AC159P_07890"
     CDS             1449..2267
                     /locus_tag="AC159P_07890"
                     /inference="COORDINATES: protein motif:HMM:TIGR00259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtpA family protein"
                     /protein_id="HIR3695412.1"
                     /translation="MLTSLFERPKPVVAMIHVGALPGTPASKAGVEELAAEAAREAAL
                     YRDCGVDGIGIENMHDVPYLRGGVGPEVVAAMAVIGRAVKTVCGLPVGVQILAGANVA
                     AVAVAHAAALDFVRAEAYAFAHVADEGWIESSAAELLRYRRMTGAERVRVWADVKKKH
                     SSHAVTADIGLGATAHAVEFMRADACIVTGNVTGEPPRAEDVREVKAHSRLPVVLGSG
                     IDADNLASFYTDADAFIVGSYFKHEGHWANPIDPRRVRRFVEVLTELRTQESRQ"
     gene            complement(2399..3742)
                     /locus_tag="AC159P_07895"
     CDS             complement(2399..3742)
                     /locus_tag="AC159P_07895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695413.1"
                     /translation="MRTGTMLYGLALCAGLLLSLHPARARGGPIDAALAAQYFREARA
                     ACERDGGRLWGVSPCGPVLFVEPQTRAVVANQPDAEGQLAKQGDVLVGKLPARQPIAN
                     AAVDWAGVKWAMIVWSFLTKDEFQRVKMMAHESFHRVQHELKFPVAGGGNDHLDTEQG
                     RVWLQLEWRALRQALTSEGKARRVAVEDALAFRQRRRALFPQSDASERALEMHEGLAE
                     YTGFKLSAKSERELIDHLARHVEQCAGRPTFVGYFAYCNGPAYGVLLDAAGADWRKGL
                     TPQDDLGELLRRASKIELPADLKAAADRQAARYDDAALRAAEAEREVARQKRLAAYRA
                     RLVDGPTLLVELTVQRRVAINTSNLVPLEGVGTVYPTATVTDAWGTLEVSDGALMIHG
                     PGGRITKVYLSRPADPAARPLKGEGWTLQLNPGWTLAPGPRDGDHVLKKEVGSRR"
     gene            4039..>5363
                     /gene="dnaE"
                     /locus_tag="AC159P_07900"
     CDS             4039..>5363
                     /gene="dnaE"
                     /locus_tag="AC159P_07900"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HIR3695414.1"
                     /translation="MSNNNDFAHLHLHTDYSLLDGAIQIKPLAERVRELGMTACAMTD
                     HGNIYGAVSFYSEMKKHEIKPLIGCEVYLAPNSRHDRKEAQKGEKPYNHMILLAKDRE
                     GYHNLVRLTSKAFKEGFYRKPRIDRELLAEFGGGLIGLSACLSGVPQAHLKAGRLDAA
                     EKAAGEFQEILGKGNYFLEIQDHQLPDQQKINKDLCELSKRSGIPLVATNDAHYLYRE
                     DYEAHDVMLCIGTGRTVNDTDRMRFGGPQWYVRSPQEMREIFTYCPDAVARTAEVAEM
                     CNLKLPLGENHLPVFPIPPAEGDISIDDYFEKVVRQGYEKRRETVWDSMRARGALQHD
                     PHEYQARVSREIEMIKRMGFPSYFLIVWDFIKFAKDRGIPVGPGRGSAAGSLVAYCLE
                     ITDVDPLQYDLLFERFLNPDRVSMPDIDIDFCVHGRSEVINHVTQLYGR"
BASE COUNT          874 a         1803 c         1822 g          864 t
ORIGIN      
        1 ctcatctccc gcgcgcgacg atctcggcga tcttcgcgtt ggcctgcgtc atggcctctt
       61 cgggcgagag ttcgccgccg ttggctttgg agaggtagac gccgaaggcg ttctcgatct
      121 cgttccagag cggggtgcgt gggcgcgggc gggaggtttc gagcgagcgg agctgcgccg
      181 ggtaggaggg gaacttggcc accagctccg cgtccgtgaa gacggcgcgg cgcgtgggcg
      241 ggttgccgcg cagcgccgag cgcttcatct cggcggcgct cgtggcccag aggagaaagt
      301 cgaaggcggc ctccgcgttg cgcgaggcgc ccgggatggc gacgagccag ttgccgatct
      361 cggcgcggcc cgcattgcgc gcgcccggca tcgtggtgaa ctccatgcgg cccgtgacct
      421 gcgagcgcgc ggtgtccgcg aaggcgccga tccacgcggg ccagttgatg gacatcgcgg
      481 cggtgccctg caggaggtgc gccgagacct cgtcggcgtt gaagctcgcg tagccgggcg
      541 gcgcgtactt gccgagttcg agcatgaacc ggagggccgc gacgccttcg gcgctgttga
      601 cggtggggcg cccttcggcg tcgaacatct cggcgccgaa ggcccagaag atcggcatga
      661 agtcggcgac gacggcgttc ccctgtgcgg cgcgcatcac gtagccgtgg acccgcccgc
      721 ccgagggcga gcctgccgcc tcgcgctcgt ggatggcgcg cgcggcggcg agcacgtcgt
      781 cccaagtggc gggcggttcg agttggtgct tctcgaagag gtcgcggcgg tagaagaaga
      841 gctgcgagtt gccgacgtaa ggcagcgcgt agagcgtgcc cgtctcgtac ggctcgcggc
      901 agagcgcgag cgaggtcggc acgaagtccg cgtccggccc cggcaagttc ttcttcgcga
      961 agagcggcgc gaggtctgtc aaaaagtcgc gcgtggcgaa gcgcgggacc cacgggtcgt
     1021 cgagcatgat gaggtcgtag gcgcccgtcg ccgcttgcag gtcaaccagc tccttctcga
     1081 agaggttggc gtacgggaac tccgcgatgc ggacgcggac gccggtctgc gcctcgtagt
     1141 cgcgcgccgc ggccttgagc gcgtcgccct cgacgccgga gttgacggcg aggctcagct
     1201 ccttcgcgcc gccgccgctg tcactgcccg cgcacccgcc cagcggcgcg agcgcgaggg
     1261 cgacgacgag gaggacggcg atgatgttcg ggaggcagtt tggtctctgt cgcatgcggg
     1321 tacggatgac ggtcgtcaat ctcaggcgcg aatcttatac accgccgcgg tcttcggcgg
     1381 aaaggataaa gacccggccc gcgccatttt cgattgacat agcggcccgc ggggcttagt
     1441 gaatagtcat gctcacttcc cttttcgaga gaccgaagcc agtcgtcgcg atgatccacg
     1501 tcggcgcgct gccgggcacg cccgcgagca aggcgggcgt cgaggagctg gcggcggagg
     1561 ccgcgcgcga ggccgcgctc taccgcgact gcggcgtgga cggcatcggc atcgagaaca
     1621 tgcacgacgt gccgtacctg cgcggcgggg tggggccgga ggtggtcgcc gcgatggccg
     1681 tcatcgggcg tgcggtgaag actgtgtgcg gcttgcccgt cggcgtgcag attctcgccg
     1741 gggcgaacgt cgcggcggtc gcggtcgcgc acgccgccgc gctggacttc gtccgcgccg
     1801 aagcctacgc cttcgcgcac gtcgcggacg agggctggat cgagtcgtcc gccgccgagc
     1861 tgctccgcta ccgccggatg accggcgccg agcgcgtccg tgtctgggcc gacgtgaaga
     1921 agaagcactc ttcgcacgcc gtcaccgcag acatcggcct cggggcgacc gcccacgcgg
     1981 tcgagttcat gcgcgcagat gcctgcatcg tcaccggcaa cgtcaccggc gagccgcccc
     2041 gcgccgagga cgtgcgcgag gtcaaagcgc actcgcgcct gccggtcgtc ctcggctccg
     2101 gcatcgacgc cgacaacctc gcctccttct acacggacgc cgacgccttc atcgtcggct
     2161 cctacttcaa acacgaaggc cactgggcca accccatcga tccgcgccgc gtccgccgct
     2221 tcgtcgaggt cttgaccgaa ttgcgaacac aagaaagtag gcagtaggca gtaggcagta
     2281 ggcagtaaaa gcgagaggct cgagagtcaa cgtctcaagc ctctcgcttt gttgcttgaa
     2341 gagtgaagat gcttggcact ttacctctca agcatctccg tcattccgtc ttgactgtct
     2401 accgcctact gcctacttct ttcttgagaa cgtggtcgcc gtcgcgtggg ccgggcgcga
     2461 gcgtccagcc ggggttgagt tggagcgtcc agccttcgcc cttgagcggg cgcgctgcgg
     2521 ggtcggcggg gcgggagagg tagaccttcg tgatgcgtcc gccggggccg tggatcatga
     2581 gcgcgccgtc ggagacttcc agcgtgcccc acgcatcggt gacggtggcg gtggggtaga
     2641 ccgtgccgac cccttcgagc gggacgaggt tgctcgtgtt gatggcgacg cgccgctgga
     2701 ccgtgagttc gacgagcagc gtcggcccgt ccacgagtcg cgcgcggtag gcggcgaggc
     2761 gtttctgccg cgcgacctcg cgctcggcct ccgccgccct gagcgcggcg tcgtcgtacc
     2821 gcgcggcctg cctgtcagcc gccgctttca ggtcggcggg cagctcgatc ttcgacgccc
     2881 tccgcaacag ctcgccgagg tcgtcctgcg gcgtcaagcc cttgcgccag tcggcgcccg
     2941 ccgcgtcgag cagcacgccg taggcggggc cgttgcagta ggcgaagtag ccgacgaagg
     3001 tcggcctgcc ggcacactgc tcgacgtgcc gcgcgagatg gtcgatgagt tcccgctcgg
     3061 acttcgcgct cagtttgaag cccgtgtact cggcgagacc ttcgtgcatc tcaagcgcgc
     3121 gctcggaggc gtccgactgc gggaagagcg cgcgccgccg ttggcggaag gcgagcgcgt
     3181 cttcgaccgc gacgcgacgc gccttgccct cgctcgtcaa agcctgccgc agcgcccgcc
     3241 actcaagctg tagccagacg cgcccctgct ccgtgtcgag gtggtcgttg ccgccgccgg
     3301 ccacggggaa cttcagttcg tgctggacgc ggtggaacga ctcgtgggcc atcatcttga
     3361 cgcgttggaa ctcgtctttc gtcaggaacg accagacgat catcgcccac ttcacgcccg
     3421 cccaatcgac ggcggcgttc gcaatgggct gccgcgcggg cagcttcccg acgagcacgt
     3481 ctccctgttt agcgagctgc ccttcggcgt ccggctgatt cgccaccacc gcacgcgtct
     3541 gcggctcgac gaacagcacg gggccgcacg gcgacacgcc ccacagcctg ccgccgtcgc
     3601 gctcgcacgc cgcgcgcgcc tcgcggaaat actgcgcggc caacgccgcg tcgatcggcc
     3661 cgccccgcgc ccgcgccgga tgaagcgaca acaggagacc ggcgcagagc gcgaggccgt
     3721 agagcatggt tccggttcgc ataacgctga tacgcaaccg gcggccaaga ggtttaaccc
     3781 ccggcgcgcc cctgtatgcg aagccggtct tcgccgaccg gcttcgcctg taagttcggg
     3841 gtcaggcaca agatgctgag cctaaatggc gaaagctgtc ccttattttg tagccgtttc
     3901 tgagtcacag gcaaagtcct cttgactctt cgtggcggat ggcttaaaaa ctcgcagcct
     3961 tcgaacggga cgcacgccgc cgagcggcgt cgcgaaatca agacaagggg tcggcccgcg
     4021 gttccacgcc atcgctgcat gtcaaacaac aacgacttcg cacacctgca tttgcacacc
     4081 gattattcgt tactggacgg ggccatccag atcaagccgc tcgccgaaag agtcagggaa
     4141 ctcgggatga cggcgtgcgc gatgaccgac cacgggaaca tctacggcgc ggtctccttc
     4201 tacagcgaga tgaagaagca cgagatcaag ccgctcatcg gctgcgaggt gtatctcgcg
     4261 ccgaacagcc ggcacgaccg gaaggaggcg cagaagggcg agaagccgta caaccacatg
     4321 atcctgctgg cgaaagaccg cgagggttat cacaacctcg tgcgcctcac gtcgaaggcg
     4381 ttcaaggaag gcttctaccg caagccgcgc atcgaccgcg agctgctcgc cgagttcggc
     4441 ggcggcctca tcggcctctc cgcgtgtctg tcgggcgtgc cgcaggcgca cctcaaggcg
     4501 ggccggctcg acgccgccga gaaggcggcg ggcgagtttc aggaaatcct gggcaagggc
     4561 aactacttcc ttgagattca ggatcaccag ctccccgacc agcagaagat caacaaggat
     4621 ttgtgcgagc tgtcgaagcg gtcgggcatc ccgctcgtcg ccacgaacga cgcgcactac
     4681 ctctaccgcg aggactacga ggcgcacgac gtgatgctct gcatcggcac ggggcgcacc
     4741 gtcaacgaca ccgaccgcat gcgcttcggc ggcccgcagt ggtacgtccg ctcgccccag
     4801 gagatgcgcg agattttcac ctactgcccc gacgccgtcg cgcgcaccgc cgaggtcgcc
     4861 gagatgtgca acctcaagct gccgctcggc gaaaaccacc tgcccgtctt ccccatcccg
     4921 cccgccgagg gcgacatctc catcgacgac tacttcgaga aggtcgtccg gcagggctac
     4981 gagaagcggc gcgaaaccgt gtgggactcg atgcgggcgc gcggcgcgct ccagcacgac
     5041 ccgcacgagt accaggcccg cgtctcgcgc gagatcgaga tgatcaagcg gatgggcttt
     5101 ccgagctact tcctcattgt ctgggacttc atcaagttcg ccaaagaccg cggcatcccc
     5161 gtcgggccgg gccgcggctc ggcggcggga agtctggtcg cctactgcct ggaaatcacc
     5221 gacgttgacc cgctgcaata cgacctgctg ttcgagcgct tcctcaaccc cgaccgcgtc
     5281 tcgatgcccg acatcgacat cgacttctgc gtccacggac gctcggaggt catcaaccac
     5341 gtcacgcagt tgtatggacg tga
//
LOCUS       DBNGOH010000399         4143 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4093884, whole genome shotgun sequence.
ACCESSION   DBNGOH010000399 DBNGOH010000000
VERSION     DBNGOH010000399.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4143)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4143)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4143
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4093884"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..909
                     /locus_tag="AC159P_07905"
     CDS             <1..909
                     /locus_tag="AC159P_07905"
                     /inference="COORDINATES: protein motif:HMM:NF012956.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HIR3695415.1"
                     /translation="PAAVRLTPRMGPKGFKADGADLAMVDVEVVDAAGNRCPVALNMI
                     DFSLAGPAEWRGGIAQGPDNYILSKSLPVENGVNRVLLRSTTEAGKVVVTATSAGLKP
                     ASVELVSRPVKVADGLSHEMPGDGLPSFEGRGPTPRGDSVTVTRRAVQIASATAGANA
                     DKAATSFDDNEVTSWSNDGRRATAWIRYELARPAKVSEVTLKLGNWRARSYPVRVTVD
                     DKVVYTGVTPQSLGYVTITFAPVTGRSLRIELTAAAAERDAFGQVTELADPQNAETTG
                     GKGDAKGSLDIVEIEIYEPVARGARR"
     gene            963..2426
                     /locus_tag="AC159P_07910"
     CDS             963..2426
                     /locus_tag="AC159P_07910"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012516.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HIR3695416.1"
                     /translation="MLSKTYGRALVSMLMVCVACGVAAGAQARGRTSAQADGLPAWVK
                     SVGARRSPQRRRVCAANAHGAVGDGATNSTRAIQKAIDTCARAGGGTVSFKPGEYVTG
                     ALFVKSNVHLRVDKGVALLGSQEDADYPSIFTRVAGIEMKWPAALINVNDQRNVKISG
                     GGTIDGRGQKWWDKYWALRREYEPKGLRWASDYDAERVRLLVVWRSADVTVENLSLRR
                     SGFWTVQVVYSDHVTVDGIKISDNGGPSTDGVDIDSSSHVLVQNCDIDNNDDDICLKA
                     GRDSDGLRVARPTEYVLIRNNITRRGGGIISFGSETSGGIRHVVGYGNRGIGTKEGIR
                     FKSAKTRGGYVEDVLIRDTKMENVPLPFTFTLNWNPSYSYATIPKDVQNPPAHWVTLN
                     TPVLPAERGFATFRRIRIENVEIVGADRIFTASGLPEKMLSDVVFTNVTARGKEAGFI
                     EYAHNWTMRNVRLHTANGEPLKITNSSNVDKPEVRKQ"
     gene            2438..3994
                     /locus_tag="AC159P_07915"
     CDS             2438..3994
                     /locus_tag="AC159P_07915"
                     /inference="COORDINATES: protein motif:HMM:NF015585.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HIR3695417.1"
                     /translation="MKTTYGLLFVAASMFLSTAASRADVTLPDVLSSGMVLQRGMAVP
                     VWGKAAPGEEVTVRFAGQTKKATAGADGRWRVVLSALKASAAPASMTIAGRNTVELKD
                     VLVGEVWLVSGQSNMQWRLFESTGGEAAVAAADHPTIRLFNVSREVGFKRKPPPLAVW
                     QATTPASAREFSAAGYYFAVELQRELKVPVGVINSSYGGSQAEAWTPVEYLLASEDLK
                     PTVERTKIWEAERPRVRAEYDEQIKQWRAASEKAKAEGARPQPSPSVPDALRDYRVAS
                     SIYNGMVEPLLPFALRGAVWYQGESNEARAEQYGLLLPTMIRAWRERWGQGDFHFGIV
                     QLPNYRRPSDVPTDEAWSHIREAQRRTARATPKTGLIVTIDLGEANDIHPRNKLDVGR
                     RMARWALADAYGRKLTRGGPSFKTAKVEGSKIVLTFEDVGQGLKIRDGDRLEEFAVAD
                     ADRKWHWAEAKIVGRDKVEVSSSAVPHPQAARYAFNNNPRRPNLTNDTGIPAAPFRTD
                     DWPGPTDGKR"
BASE COUNT          747 a         1365 c         1475 g          556 t
ORIGIN      
        1 cccgccgccg tccgcctcac gccgcgcatg gggccgaagg ggttcaaggc cgacggcgca
       61 gacctcgcga tggtggatgt cgaggtggtg gacgccgcag ggaaccgctg ccccgtggcc
      121 ctcaacatga tcgacttctc gctcgcaggc ccggcggagt ggcgcggcgg catcgcgcag
      181 gggccggaca actacatcct ctcgaagagc ctgccggtcg agaacggggt caaccgcgtg
      241 ctcctgcgct cgacgactga ggccgggaag gtcgtcgtca cggcaacttc ggcgggcctc
      301 aagccggcgt cggtcgagtt ggtgtcgcgc cccgtgaagg tcgcggacgg cctctcgcac
      361 gaaatgccgg gcgacgggct gccgtcgttc gagggccgcg ggccgacgcc gcgcggcgac
      421 tccgtgacgg tgacgcgcag ggccgtccaa atcgcgtccg cgacggcggg cgcgaacgcg
      481 gacaaggcgg ccacgagctt cgacgacaac gaggtcacga gctggtcgaa cgacgggcgg
      541 cgcgcgaccg cgtggatcag gtacgagctg gcgcggcccg caaaggtctc cgaggtgacg
      601 ctcaagctgg gcaattggcg ggcgcgctcc taccccgtcc gcgtcacggt tgacgacaag
      661 gtcgtgtaca cgggcgtcac cccgcagagc ctcggctacg tgaccatcac gttcgcgccc
      721 gtgaccggca ggagcctccg catagagctg accgccgccg ccgccgagcg cgacgccttc
      781 gggcaagtca ccgaactcgc cgacccgcag aacgcggaga ccaccggcgg caagggcgac
      841 gcgaagggaa gcctcgacat cgtcgagata gagatttacg agccggtcgc gcgcggcgcg
      901 cgccgttgag cgggccgggc ggacgcgcaa gagcggataa acccagagga agcgggaaga
      961 caatgctgag caagacatac gggcgggcgc tggtatcgat gttgatggtg tgcgtggcgt
     1021 gcggcgtcgc cgccggcgcg caggcgcggg gccgaacgtc ggcgcaggcg gacggcctgc
     1081 ccgcgtgggt gaagagcgtc ggggcgcggc gctccccgca gaggcggcgc gtgtgcgcgg
     1141 cgaacgcgca cggcgcggtc ggcgacggcg cgaccaactc gacccgcgcg attcagaagg
     1201 cgatcgacac ctgcgcccgc gcgggcggag gcacggtcag cttcaagccg ggcgagtacg
     1261 tgaccggcgc gctcttcgtg aagagcaacg tgcacctccg ggttgacaag ggcgtcgcgc
     1321 tgctcggcag ccaggaggac gccgactacc cctcgatctt cacgcgcgtc gccggcatcg
     1381 agatgaagtg gcccgccgcg ctgatcaacg tcaacgacca gcgcaacgtc aaaatctcgg
     1441 gcgggggcac gatcgacggg cgcgggcaga agtggtggga caagtattgg gccttgcggc
     1501 gcgagtacga gccgaagggc ttgcgctggg cctcggacta cgacgccgag cgcgtgcggc
     1561 tgctggtcgt ctggcgctcg gcggacgtga cggtcgagaa cctgagcctc cggcgctcgg
     1621 gcttctggac ggtgcaggtc gtctactccg accacgtcac cgtggacggc atcaagatca
     1681 gcgacaacgg cggcccgagc acggacggcg tggacatcga ctcttcgagc cacgtgctgg
     1741 tgcagaactg cgacatcgac aacaacgacg acgacatctg tctgaaggcg ggccgcgact
     1801 cggacggcct gcgcgtcgcg cggcccaccg agtacgtcct catccgcaac aacatcacgc
     1861 ggcgcggcgg cggcatcatc tccttcggca gcgaaacctc gggcggcatc cgccacgtcg
     1921 tcggctacgg caaccgcggc atcggcacca aagagggcat ccgcttcaag tccgcgaaga
     1981 cgcgcggcgg ctacgtcgag gacgttctga tccgcgacac gaagatggag aacgtgccgc
     2041 tccccttcac cttcacgctc aactggaacc cgagctactc gtacgcgacc atcccgaagg
     2101 acgtgcagaa cccgcccgcc cactgggtca cgctgaacac gcccgtgctg cccgccgagc
     2161 gcggcttcgc caccttccgc cgcatccgca tcgagaacgt cgagatcgtg ggcgccgacc
     2221 gcatcttcac ggcgagcggc ctgcccgaga agatgctgag cgacgtggtg ttcacgaacg
     2281 tcacggcgcg cggcaaggag gccggcttca tcgagtacgc gcacaactgg acgatgcgca
     2341 acgtcaggct gcacaccgcg aacggcgagc cgttgaagat caccaacagc agcaacgtcg
     2401 ataagccgga ggtgaggaag caatgaggcg tgcgcacatg aaaacgacgt acggacttct
     2461 cttcgtcgcg gcgtcgatgt tcttgtcaac ggcagcttcg cgcgcggacg tgacgctgcc
     2521 cgacgtgctc tcctccggca tggtgctgca acgcggaatg gccgtgcccg tgtgggggaa
     2581 ggcggcgccg ggcgaggaag tgaccgtcag gttcgcgggg cagacgaaga aggcgaccgc
     2641 gggcgcggac ggcaggtggc gcgtcgtgct cagcgctttg aaggcgagcg cggcgcccgc
     2701 ctcgatgaca atcgcgggcc ggaacaccgt cgagttgaag gacgtgctgg tcggggaggt
     2761 gtggctggtg tcggggcagt cgaacatgca gtggcgcctg ttcgaatcga ccggcggcga
     2821 ggcggcggtc gccgccgccg accacccgac aattcgcctc ttcaacgtca gccgcgaggt
     2881 cggtttcaag cgcaaaccgc cgccgctcgc cgtgtggcag gcgacgacgc ccgcctccgc
     2941 gcgcgagttc tccgccgcgg gctattactt cgccgtcgag ttgcagcggg agttgaaggt
     3001 gcccgtcggc gtcatcaact cttcctacgg cggctcgcag gcggaggctt ggacgccggt
     3061 cgagtacctg ctcgcgtccg aggatttgaa gcccacggtc gagcgcacga aaatctggga
     3121 ggccgaaagg ccgcgcgtgc gcgccgagta cgacgagcag atcaaacagt ggcgcgcggc
     3181 ctccgagaag gccaaggcgg agggcgcgag gccgcagcct tcgccctccg tgcccgacgc
     3241 tttgcgcgac taccgcgtcg cgtcttcgat ctacaacggc atggtcgagc cgctactccc
     3301 cttcgcgctg cgcggcgccg tctggtatca gggcgagtcg aacgaggcgc gcgcagagca
     3361 gtacgggttg ctgctgccga cgatgattcg cgcgtggcgc gaacgctggg ggcagggcga
     3421 cttccacttc ggcatcgtgc agttgcccaa ctaccgccgc ccgtccgacg taccgaccga
     3481 cgaggcgtgg agccacatcc gcgaggcgca gcggcgcacc gcgcgcgcga cgccgaagac
     3541 cggccttatc gtcaccatcg acctcggcga ggcgaacgac atccacccgc gcaacaagct
     3601 cgacgtgggg cggcgcatgg cgcgctgggc gctcgccgac gcgtacgggc ggaagttgac
     3661 gaggggcggc ccttcgttca agacggcgaa ggtggaaggc tcgaaaatcg tgctcacgtt
     3721 cgaagacgtg gggcaagggt tgaagatacg cgacggcgac cggctcgaag agttcgccgt
     3781 cgcggacgcc gaccgcaagt ggcactgggc cgaggcgaag attgtcggca gagacaaggt
     3841 cgaggtctcg tcttccgccg tgccgcaccc gcaggccgcg cgctacgcct tcaacaacaa
     3901 cccgcgccgc ccgaacctga cgaacgacac aggcatcccc gccgccccgt tccgcaccga
     3961 cgactggccc ggcccgaccg acggcaagcg ttagcctgtc cgattacaac gaagggaagc
     4021 gaacacagat gcaactcctc gccaagctga agcggtttgc ggttgagtgt aaccggaggc
     4081 aggattaaac gcagaggacg cggtggggcg cagaggacgc agagaaggag ttaatggctt
     4141 tct
//
LOCUS       DBNGOH010000400         2575 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_61537, whole genome shotgun sequence.
ACCESSION   DBNGOH010000400 DBNGOH010000000
VERSION     DBNGOH010000400.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2575)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2575)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2575
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_61537"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="AC159P_07920"
     CDS             <1..189
                     /locus_tag="AC159P_07920"
                     /inference="COORDINATES: protein motif:HMM:NF015648.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HIR3695418.1"
                     /translation="RNIPLRNIQDVTVKTTILQRLLRFGNVVVDNASEEGGVTVLRDI
                     PDPRRHADLLLRELRRWR"
     gene            322..1497
                     /locus_tag="AC159P_07925"
     CDS             322..1497
                     /locus_tag="AC159P_07925"
                     /inference="COORDINATES: protein motif:HMM:NF042697.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6599 family protein"
                     /protein_id="HIR3695419.1"
                     /translation="MGLSSLPKLFSTVALLALLLVTAQAQSKPDDAAAKLLPARIGDF
                     SAQGAPRSEASEIFGRVKAEDFGVTSQARRDYVSADGGKLSILLVKTGSVASAYSLLT
                     HVASAYSPPQSSPIKLGDVGTASVAGPGQVSFAKGATFVQIADTGDEENKAAPTDFAR
                     LFAASFESGGELPVLVLHLPEWEKTHQAAVYAVSLPALKHAAGDTPVLDALSFDGGAE
                     AVTAKYGDARLVIVEYTTPQYAVENDNRINERIAQLRGAGQSVPTAYKRVGNYSVFVF
                     DARDAAEAERLLAGVKYEKDVRWLGRNPHGDEIAQRAYTNTMTSVVVTVFKTSGLAIA
                     LCLGIGAVFGGIVFIRRRARVSAVHAYSDGGEMLRLNLLEDVAAPQPTANLLGQGKE"
     gene            complement(2039..>2575)
                     /locus_tag="AC159P_07930"
     CDS             complement(2039..>2575)
                     /locus_tag="AC159P_07930"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF017864.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HIR3695420.1"
                     /translation="AVRTVSQFSGFPVEDIAVGVDGCGVPVFGLTVRAMALMYARLVA
                     PPESFDAETRDACARLTAAMNAHPELVGGTRERFDTEVMRAAAGRVVSKVGAEGVYTA
                     GVLPCEEWPKGMGLAFKIEDGEDRRARPTVAIEALRQLGVLDEKALGALAPYSVFPIN
                     NHRGDRVGEIRPNFKLQE"
BASE COUNT          480 a          881 c          759 g          455 t
ORIGIN      
        1 cgcaacatcc ctttgcgcaa cattcaggac gtgacggtca agacgaccat cctccagcgc
       61 ctgctcaggt tcggcaacgt cgtcgtcgat aacgcgagcg aggagggcgg cgtcaccgtc
      121 ctgcgcgaca tccccgaccc gcgccgccac gcagacctcc tcctgcgcga actccgccgc
      181 tggcgctgac aggccgcgcc gccttatcat cgaccgcggc ggaaccaaca caacggcggc
      241 ggtgtctaaa acatccaaag attttcgccg ggggcgcttg tgcctgttgc cggcgcaccg
      301 ttacgtccga aaggttttat cgtgggtctt tcatctctac cgaagctgtt ctcgacagtc
      361 gccctcttgg cgctcctgct cgtcacggca caggcacagt cgaagccgga cgacgccgcc
      421 gcgaagttgc tgcccgcgcg aatcggcgac tttagcgcgc agggcgcccc ccgttcggag
      481 gcgtccgaga ttttcgggcg cgtgaaggcc gaagacttcg gcgttacttc ccaagcccgc
      541 cgcgactacg tttcggcgga cggcgggaag ctcagcatcc tcctcgtcaa gacgggttcg
      601 gtcgccagcg cgtattctct cctgacacac gtcgcttccg cctactcccc gccgcagtct
      661 tcgcccatca agctgggcga tgtcgggacg gccagcgtgg cggggccggg gcaggtctct
      721 tttgcgaagg gcgccacgtt cgtgcagatc gcggacaccg gcgacgagga gaacaaggcc
      781 gcgccgacgg actttgcaag gctgttcgcc gcgagtttcg agtcgggcgg cgagctgccc
      841 gtcctcgtcc tgcaccttcc cgagtgggaa aagacgcatc aggcagccgt ctacgccgtg
      901 agcctgcccg cgctgaaaca cgcagccggc gacacgcccg tcctcgacgc gctctccttc
      961 gacggcggcg ccgaggccgt cacggcgaag tacggcgacg cgcgactcgt catcgtcgag
     1021 tacaccacgc cgcagtacgc cgtcgagaac gacaaccgaa tcaacgagcg gatcgcgcag
     1081 ctccgcggcg cgggccaatc ggtgccgacg gcctacaagc gcgtcggcaa ctattcggtc
     1141 ttcgtcttcg acgcgaggga cgccgcggag gcggagcggc tgctggccgg ggtgaagtac
     1201 gagaaggacg tgcgctggct gggccgcaac ccgcacggcg acgagatcgc acagcgcgcc
     1261 tacacgaaca cgatgacgag cgtggtcgtg accgtcttca agacctcggg cctcgccatc
     1321 gcgctgtgcc tcggcatcgg ggccgtcttc ggcggcatcg tcttcatccg ccgccgcgcg
     1381 cgcgtctcag ccgtgcatgc ctactcggac ggcggcgaga tgctgcgcct gaatctcctg
     1441 gaagatgtgg ccgcgccgca gcccacggcc aacctgctcg gtcaaggcaa agagtaagtc
     1501 cgcgaacgac cgaccgccgc aaaaaaacat aagcgccgcg agcatcaagc tcacggcgct
     1561 ttaaaatttc aggagtaagt tcgggacaaa agaacgcggg ttgctgactt tactgcgggg
     1621 ttcccccaaa gcccctacag accgaatcag caacccgctg aacaatgccc ttcgaccgct
     1681 atgccggcaa ccactccgtt ccccctaaga gtcgttgtcg gcgttttatg taagagaaaa
     1741 cctcccccaa agttttcact tactagccgt ctccgagcct gacccgataa ctcaatttac
     1801 cccctactat tttatctgat ctcccttttg caacgcctgt gccactacga ctccgctcac
     1861 aatatccctt tttgaccgac agttttcgtc agtaattaag cggctttcgt ccatctgctt
     1921 gttaaaagag gcgcttactt aagtggacta aaagcgccga attgcgtacc cattcgatac
     1981 taacgactgt acacttgtct gcaaacgttg cagcttaacg cctttgagcg cttacgtact
     2041 attcttgcaa cttgaagtta gggcgaatct cgccgaccct gtcgcccctg tgattgttga
     2101 tgggaaagac ggaataaggc gcgagcgcgc ccagtgcttt ttcgtccaga acgcccaact
     2161 gtcgtagggc ttcgatggcg accgtgggcc gcgcgcgacg gtcttcgccg tcctcgatct
     2221 tgaaggccag ccccataccc ttcggccact cctcgcacgg cagcacgccc gccgtgtaga
     2281 cgccctccgc cccgaccttc gagacgacgc gccccgcggc ggcgcgcatc acctccgtat
     2341 cgaaacgctc gcgcgtcccg ccgaccaact ccggatgcgc gttcatggcc gcggtgaggc
     2401 gcgcacaggc gtcgcgagtc tccgcgtcaa aactctcggg gggcgcgacg aggcgcgcgt
     2461 acatgagcgc catcgcgcgc accgtgaggc cgaagacggg cacgccgcag ccgtccacgc
     2521 cgacggcgat gtcctcgacg gggaagcccg agaactgcga gacggtgcgg acggc
//
LOCUS       DBNGOH010000401         5100 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_5010361, whole genome shotgun sequence.
ACCESSION   DBNGOH010000401 DBNGOH010000000
VERSION     DBNGOH010000401.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5100)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5100)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5100
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_5010361"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1086)
                     /locus_tag="AC159P_07935"
     CDS             complement(<1..1086)
                     /locus_tag="AC159P_07935"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase domain-containing
                     protein"
                     /protein_id="HIR3695421.1"
                     /translation="MNNVRSRAITRGVERAPNRAMLRAVGFGDGDFEKPIIGVANGHS
                     TITPCNMGLNVLTERAVAALSAAGAMPQTFGTVTVSDGISMGTEGMKYSLVSREVIAD
                     AIETACMGQSMDGVLAVGGCDKNMPGAMIAIARMNIPAIFVYGGTIKAGHYQGQDLTI
                     VSAFEAVGQYCAHRISESELLEVERRACPGAGSCGGMYTANTMSSAIEAMGLSLPYSS
                     TMAAEDDEKAENAARSAEVLAEAVRKEILPRQLLTREAFENAMAVVMAVGGSTNAVLH
                     LLAIAHAAEVPLALEDFEEVRARVPVLCDLKPSGRYVATDLHAAGGIPQVMKMLLAAG
                     LLHGDALTITGETIAEVLAAVPAAPRAD"
     gene            complement(1161..2498)
                     /locus_tag="AC159P_07940"
     CDS             complement(1161..2498)
                     /locus_tag="AC159P_07940"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015125291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0055129 - L-proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HIR3695422.1"
                     /translation="MSSFAVETVSQTSVAEVARRAKAASRRLAVLPGGRRAAALLSAA
                     LAIEERRAEILAANRGDLLDAEREAEAGRMSPSILKRLRTDERGVAEMAARVRDVAAL
                     ADPLGRELAATELDDGLTLHKVSCPLGVVGIVFEARPEVVPQVASLALKSGNAVILKG
                     GSEAARTNEALARIWRDALAHFPDIPADSIGLLRTREDVTALLKLDDMIDLIIPRGSK
                     EFVNYIAAHSRVPVLGHGEGVCHVYIDRAADVRKAVAVAVDSKAQYPAACNAAETLLV
                     HEEVAARLLPELVGALRAAGVEVRACPRTASHVAAGETVAAGEDDWATEYSDLVVSIK
                     TVGSFEEAVRHVERYGSRHTECIVTEDPDAAARFMETVDAAGVYHNASTRFADGFRYG
                     LGAELGISTAKLHARGPVGLDGLTTYKYKLVGDGHTVAEYSSGERTFKHRELK"
     gene            complement(2702..3913)
                     /gene="proB"
                     /locus_tag="AC159P_07945"
     CDS             complement(2702..3913)
                     /gene="proB"
                     /locus_tag="AC159P_07945"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0055129 - L-proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HIR3695423.1"
                     /translation="MEVEDLQTERRRRLREARRAVVKVGTSVVSGAAGEVCAERVGPI
                     VRAVAALRGEGRGVVLVSSGAVGLGAGRLGLSRARLGDVVVRQACAAVGQSLLMHAYE
                     RMFGECGVKIAQVLLTEDDFTDRRRYSNLQRTMEKLLKLGVVPVVNENDTVSTAELEY
                     VGAGRVFSDNDRLAALVTSKLESDALVLLTDVDGLLVHRPPDVLAGAARDEEVMVVPL
                     VAEVTPELKALATGASATGRGGMLTKLEAAAIAMRAGGVAVIANGTRPDTLERVFAGE
                     AVGTAFVPTSRMGGKRRWIAYAAGVRGRVVVNAGARAAILGGKASLLASGVVRVEREF
                     EPSDVVSIAGPEGREFARGIANCASADTIALINRADADDARAAKHATRASVLVTRDNI
                     VILEGQPETIR"
     gene            complement(3996..5012)
                     /gene="ilvC"
                     /locus_tag="AC159P_07950"
     CDS             complement(3996..5012)
                     /gene="ilvC"
                     /locus_tag="AC159P_07950"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HIR3695424.1"
                     /translation="MKVYYDKDANLNALRERRIAVIGYGSQGFAQSNNLKDSGCDVVV
                     GLRPDSPTRAKAEKAGLKVLPVPQAAAEADLVMMLVPDEQAADVYARDIAESMTPGKY
                     LAFSHGFSIHYKFIQPPEDVNVLMVAPKGPGHLVRSEYARGAGVPCLLAVNQDPTGDA
                     RELGLAYASAIGGGRAGIIETNFKEETETDLFGEQAVLCGGLTSLIKAGFETLVEAGY
                     APEMAYFECLHEMKLIVDLIYEGGISEMRYSVSNTAQYGDMTRGPVVVNDETKQRMRG
                     LLAEIQSGVFANEWMRENRNGKERFRRLERSTNEHQIERVGKGLREMMPWLAANRLVD
                     ETTN"
BASE COUNT          774 a         1849 c         1609 g          868 t
ORIGIN      
        1 atcggcgcgt ggcgcggcgg gcactgcggc gagcacttcg gcgattgttt cgcccgtgat
       61 ggtcagcgcg tcgccgtgca gcaggcccgc cgcgagcagc atcttcatca cctgcgggat
      121 gccgcccgcc gcgtgcaggt cggtggcgac gtagcggccc gagggcttca aatcgcagag
      181 cacggggacg cgcgctcgca cctcttcgaa gtcttcgagc gcgagcggca cctccgccgc
      241 gtgcgcgatg gccaacaggt ggagcaccgc gttcgtcgag ccgccgaccg ccatcaccac
      301 cgccatcgcg ttctcgaacg cctcgcgcgt caggagctga cgcggcagaa tctccttccg
      361 caccgcctcg gccagcacct ccgccgagcg ggcagcgttc tccgccttct cgtcgtcctc
      421 ggcggccatc gtcgaagagt aagggaggct caggcccatc gcctcgatgg ccgaagacat
      481 cgtgttcgcc gtgtacatcc cgccgcacga acccgcgccc gggcacgcgc ggcgctcgac
      541 ttcgagcagc tccgactcgc tgatccggtg cgcgcagtac tggccgacgg cctcgaacgc
      601 gctgacgatg gtcaggtcct gaccctgata gtggccggcc ttgatcgtcc cgccgtagac
      661 gaagatggcc gggatgttca tgcgcgcgat ggcgatcatc gcgcccggca tgttcttgtc
      721 gcagccgccg acggcgagca cgccgtccat gctctgcccc atgcacgccg tctcgatggc
      781 gtcggcgatc acctcgcgcg agacgagcga gtacttcatc ccctcggtgc ccatcgagat
      841 gccgtcgctc accgtgacgg tgccgaacgt ctgcggcatc gcgcccgccg cactgagggc
      901 cgcgaccgcg cgctccgtca acacgttcaa gcccatgttg cacggcgtga tcgtgctatg
      961 gccgttcgcc acgccgatga tcggcttctc gaaatcgccg tcgccgaaac ccaccgcgcg
     1021 cagcattgca cggttcggcg cccgctcgac gccccgcgta atcgccctgc ttctcacgtt
     1081 attcataaaa ataaggactg aggactgagg actgaggact gaggaaaaac gtgttcttca
     1141 aattcagtcc tcagtcctca tcacttgagt tcccggtgtt tgaaggttcg ctcgccgctg
     1201 ctgtattcgg cgacggtgtg gccgtcgccg acgagtttgt atttgtaggt ggtgaggccg
     1261 tcgaggccga ccgggccgcg ggcgtggagc ttggcggtgc tgatgccgag ttccgcgccg
     1321 aggccgtagc ggaagccgtc ggcgaaacgc gtcgaggcgt tgtggtagac gcccgccgcg
     1381 tccaccgtct ccatgaagcg cgccgccgcg tccgggtctt cggtcacgat gcattccgtg
     1441 tggcgcgagc cgtagcgctc gacgtggcgt acggcctctt cgaagctgcc gaccgtcttg
     1501 atggagacga cgaggtcgga gtattcggtc gcccagtcgt cttcgccagc cgcgaccgtt
     1561 tcgcccgcgg cgacgtgcga agcggtgcgc gggcaggcgc ggacttcgac gcccgcggct
     1621 cgcagcgcgc cgaccagttc gggcaagagg cgcgcggcca cctcttcgtg gacgagcagc
     1681 gtctccgccg cgttgcaggc cgccgggtac tgcgccttcg aatcgacggc gacggccacg
     1741 gccttccgca cgtcagccgc gcggtcgatg tagacgtggc acacgccctc gccgtggccg
     1801 agcacgggga cgcggctgtg cgcggcgatg taattcacga actccttcga gccgcgcggg
     1861 atgatgaggt cgatcatgtc gtcgagcttg agcagcgccg tcacgtcctc gcgcgtgcgg
     1921 agcaggccga ttgaatccgc ggggatgtcg gggaagtgcg cgagcgcgtc gcgccagatg
     1981 cgggcgagcg cttcgttggt gcgcgcggcc tcgctgccgc ccttcaggat gacggcgttg
     2041 cccgacttca gcgcgagcga ggcgacctgc ggcaccacct cgggccgcgc ctcgaagacg
     2101 atgccgacca cgcccaacgg gcacgacact ttgtggagcg tcaggccgtc gtccaattcg
     2161 gtcgccgcaa gctctcggcc cagagggtct gcgagcgcgg ccacgtcgcg cacgcgcgcg
     2221 gccatctccg ccacgcccct ctcgtccgtc cgcaggcgct tcaatatcga cggcgacatc
     2281 cgccccgcct ccgcctcgcg ctccgcgtcg agcaggtcgc cccggttggc cgcgagtatc
     2341 tccgcgcgcc tctcttcgat cgccagcgcc gccgacagca gcgccgcggc gcgccgcccg
     2401 ccggggagaa ctgcgagacg gcgcgacgcg gccttcgccc gccgagccac ctcggcgacg
     2461 ctcgtttgac tcactgtttc gacggcaaag ctactcataa caggatggaa gaacgcgtcc
     2521 gttctcgccg cgtagcggcg gttgagtcac tgacccggcc ttgaaaggcc gggctacatt
     2581 catcctgccg ctacgcggcg gatcgataaa acgtcttttg aaaaactcaa ccatcaactt
     2641 tcgcctctcg attcaaccgc tcattcgtcg aatcgcctct ggcttcgatt ctactattcg
     2701 ttcaacgaat cgtctccggc tggccctcca gaatcacgat gttgtcgcgc gtgacgagca
     2761 cgctcgcccg agtcgcgtgc ttcgcggcgc gcgcgtcgtc cgcgtcggcg cggttgatga
     2821 gggcgatggt gtctgcgctc gcgcagttgg cgatgccgcg cgcgaactcg cggccctcgg
     2881 ggccggcgat gctgaccacg tccgacggct cgaactcgcg ctcgaccctg acgacgcccg
     2941 aggcgagcag gctcgccttg ccgccgagga tggcggcgcg cgcgcccgcg ttgacgacga
     3001 cgcgcccgcg cacgcccgcc gcgtacgcga tccaacggcg cttgccgccc atgcgcgacg
     3061 tggggacgaa ggcggtgccc acggcctcgc ccgcgaagac gcgttcgagc gtgtcgggcc
     3121 gcgtgccgtt ggcgatgacg gcgacgccgc ccgcgcgcat ggcgatggcc gccgcttcga
     3181 gcttcgtcaa catcccgcct ctaccggtgg ccgaagcgcc cgtcgccagc gccttcagtt
     3241 ccggcgtcac ctccgcgacc agtgggacga ccatcacctc ctcgtcgcgc gccgcgcccg
     3301 ccaacacgtc cggcgggcgg tggacgagca gcccgtccac gtccgtcagc agcacgagcg
     3361 cgtcggattc gagcttgctc gtcacgagcg cggcgagccg gtcgttgtcg ctgaagactc
     3421 ggcccgcgcc gacgtactca agctccgcgg tcgagaccgt gtcgttctcg ttgacgacgg
     3481 gcaccacgcc cagcttcaac agcttctcca tcgtgcgttg caggttggaa taacgacgcc
     3541 gatccgtgaa gtcgtcctcc gtcaacaaga cctgcgcgat cttcacgccg cactcgccga
     3601 acatcctttc gtaagcgtgc atcagcaagc tctgccccac cgccgcgcac gcctgccgca
     3661 ccaccacgtc gccgagccgc gcgcgcgaca aacccaaacg ccccgcgccc aatccgaccg
     3721 cgcccgaaga gaccagcacg accccgcgcc cctccccgcg cagcgccgcc accgcacgca
     3781 caatcggccc cacgcgctcc gcgcacacct cgcccgccgc gcccgacacc acgctcgtcc
     3841 ccaccttgac gaccgcccgc cgcgcctcgc gcaaacgccg ccgccgctcg gtttgtaaat
     3901 cctcgacttc catcttcgaa agcggtgaat ggtaaatagt gaatggtaaa tagaaaccgc
     3961 ttttcaccat ttaccattta ccattcacct tcttctcagt tcgtcgtctc gtccaccagc
     4021 ctgttggcgg cgagccaggg catcatctcg cgcaggccct tgccgacgcg ttcgatctgg
     4081 tgctcgttgg tgctacgctc cagccggcgg aagcgctcct taccgttgcg gttctcgcgc
     4141 atccactcgt tggcgaagac gccggactga atctcggcga ggagacccct catgcgctgc
     4201 ttcgtctcgt cgttgacgac gacggggccg cgcgtcatgt cgccgtactg cgccgtgttc
     4261 gagaccgagt agcgcatctc cgagatgccg ccctcgtaga tgagatcgac gatgagcttc
     4321 atctcgtgca ggcactcgaa gtaggccatc tcgggcgcgt agcccgcctc gaccagcgtc
     4381 tcgaagcccg ccttgatgag cgacgtgagg ccgccgcaca ggacggcctg ctcgccgaag
     4441 aggtcggtct ccgtctcctc cttgaagttg gtctcgatga tgccggcccg accgccgccg
     4501 atggccgaag cgtaggcgag gcccaactcg cgcgcgtcgc ccgtcgggtc ttggttcacg
     4561 gcgagcaggc acggcacgcc cgcgccgcgc gcgtactcgg agcgcaccag atggcccggc
     4621 cccttcggcg cgaccatcag cacgttcacg tcctcgggcg gctggatgaa cttgtagtgg
     4681 atcgagaagc cgtgcgagaa ggcgaggtac ttgcccggcg tcatcgactc ggcgatgtcg
     4741 cgcgcgtaca cgtccgccgc ctgctcgtcc ggcacgagca tcatcacgag gtctgcctcg
     4801 gcggcggcct gcggcaccgg cagcaccttc aggcccgcct tctccgcctt cgcccgcgtc
     4861 gggctgtcgg gcctgaggcc gacgacgaca tcgcaccccg agtccttcag gttgttcgac
     4921 tgcgcgaacc cctggctgcc gtacccgatg accgcgatgc gccgctcgcg cagcgcgttc
     4981 aagttcgcat ccttgtcgta ataaaccttc atctcttcga atcctttcga taatggttgg
     5041 ggccgggggc cgggggtcag tggccggact gaaaaacaag ctctttttct tccgggcccc
//
LOCUS       DBNGOH010000402         2860 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_428983, whole genome shotgun sequence.
ACCESSION   DBNGOH010000402 DBNGOH010000000
VERSION     DBNGOH010000402.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2860)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2860)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2860
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_428983"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2637)
                     /locus_tag="AC159P_07955"
     CDS             complement(<1..2637)
                     /locus_tag="AC159P_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015346195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HIR3695425.1"
                     /translation="MTSRFKKEGAESGPNTRGETRPGVVNLADARRTVASLATDTTAN
                     LYRELAETRELLDRGLPSAAEQRLHQLINAARRDPQLLAQSRRTLSIALEILGRYKES
                     LEVVRMYEPAEARGTLDAETFSALRVQIGIAYNYAGDHPKAVGMLSAELREAVERHGT
                     DAQLGAIYAALARVYRSINEHTIARDHISKALEHYRRNGEWRGLAEAYFGLAMADLQE
                     GHYESAIENFEQAASLVGDHPAPYLLGKIYNNLAGAYWFLKRPHDGIRALEKAVGYCE
                     RTEHKEVAATGYNNLGINLMLTGDWSRAQTALKRALEISLEIDTHGQHAAVVFDSLGE
                     LSLLRGNLDEALSYQERALRIVKEVGKKWYLAQAMRTLTRCHIALGRTDDALLSGEES
                     LSIAERIGDRQAVCESTLLLAEAHLLNDDTQESAALLQKVSTETADSATDIAVSGEAQ
                     RILGMLALRQQDAPLAAHYFGRCLSIFDMLGDRYRSARAHYWLGRADTALAQHDRAAE
                     HLGLAVNAFRELGAKLDLQRAEEARAALDASVAEEHAEPPATTQLLTLRLAEAVASRE
                     LLLRELAAVVQQETRARRVLIAEPGDEGVFKPVIGHGYTNEEGASVAAALGEAKTDED
                     RARVAEGLDARVFFLRAPNAPPAVLAVTPRAQAVLADGAPLEPLLRVVELGMDVCALR
                     TRARSGRGAEAPDSLAGASLMPGFIHSSPAMTHLVEEVHKIRSSDVTVLVTGESGTGK
                     ELVARAIHALSSRRNKVFVPFNCTAVPKELSEGYLFGYRRGAFTGAVNDSAGVIRTAA
                     GGTLFLDEIGDLPLDVQPKSLRFLQEGEIQPLGEQRPVQVDVRIIAATNTDLEQMVAD
                     GRFREDLYYRLNVIRLRVPPLRE"
BASE COUNT          405 a          905 c         1074 g          476 t
ORIGIN      
        1 ctcgcgcagg ggggggacgc gcagccggat gacgttgagc cggtaataga ggtcttcgcg
       61 gaagcgcccg tcggcgacca tctgttcgag gtcggtgttg gtggccgcga tgatgcgcac
      121 gtccacctgg acgggccgct gctcgccgag cggctgaatc tcgccctcct gcaggaacct
      181 tagactcttg ggctgcacgt cgagcggcag gtcgccgatc tcgtcgagga agagcgtgcc
      241 gccggcggcg gtgcggatga cgcccgccga gtcgttgacc gcgccggtga aggcgccgcg
      301 ccggtagccg aacagatagc cttccgacaa ctccttcggc accgcggtgc agttgaacgg
      361 gacgaagacc ttgttgcggc gcgaggagag cgcgtggatg gcgcgcgcca ccagctcctt
      421 gcccgtgccc gactcgcccg tgacgaggac ggtcacgtcc gaagagcgaa tcttgtgcac
      481 ctcctcgacg aggtgcgtca tcgcgggact cgaatggatg aagccgggca tgagcgacgc
      541 gcccgcgagc gagtcgggcg cctcggcgcc gcggcccgag cgcgcgcgcg tgcgcagcgc
      601 gcacacgtcc atgccgagtt cgacgacgcg caggagcggt tcgaggggcg ccccgtcggc
      661 gagcacggcc tgcgcgcgcg gcgtgacggc gagcacggcg ggcggcgcgt tcggcgcgcg
      721 gaggaagaag acgcgcgcgt ccagcccctc ggccacgcgc gcgcggtctt cgtccgtctt
      781 cgcttcgccg agcgcggcgg cgacggacgc gccctcttcg ttcgtgtagc cgtggccgat
      841 gacgggcttg aagacgccct cgtcgcccgg ctcggcgatg aggacgcggc gggcgcgcgt
      901 ctcctgctgg acgaccgccg ccaactccct gagcagcagc tcgcgcgagg cgacggcctc
      961 ggcgaggcgc agcgtgagca gttgggtcgt cgcgggcggc tcggcgtgtt cttcggcgac
     1021 gctcgcgtcg agcgcggccc gtgcctcttc ggcgcgctgg aggtcgagct tggcgccgag
     1081 ttcgcggaag gcgttgacgg cgaggccgag gtgttcggcg gcgcggtcgt gctgggcgag
     1141 ggcggtgtcg gcgcgaccga gccagtagtg cgcgcgggcg ctgcggtagc ggtcgccgag
     1201 catgtcgaag atcgagaggc agcgaccgaa gtagtgcgcg gcgagcggcg cgtcctgctg
     1261 ccggagcgcg agcatgccga ggatgcgctg cgcctcgccg gagacggcga tgtcggtcgc
     1321 agagtcggcg gtctcggtcg agaccttctg caagagcgcg gcggactcct gcgtgtcgtc
     1381 gttcagcagg tgcgcctcgg ccagcagcag cgtggactcg cacacggcct gacggtcgcc
     1441 gatgcgctcg gcgatggaga gcgattcctc gccgctcagc agcgcgtcgt ccgtgcgccc
     1501 gagcgcgatg tggcagcgcg tcagcgtccg catcgcctgc gccagatacc acttcttgcc
     1561 gacctctttg acgatgcgca gcgcgcgctc ctggtacgag agcgcctcgt cgaggttgcc
     1621 gcgcagcagc gacagctcgc cgagcgagtc gaagacgacc gccgcgtgct ggccgtgcgt
     1681 gtcgatctcc agagagattt cgagcgcgcg cttgagggcc gtctgcgcgc ggctccagtc
     1741 gcccgtcagc atcaggttga tgccgaggtt gttgtaaccg gtggcggcca cctccttgtg
     1801 ctcggtgcgc tcgcagtagc cgacggcctt ctccagcgcg cggatgccgt cgtgcggtcg
     1861 cttgaggaac cagtaggcgc cggcgaggtt gttgtagatt ttgccgagca ggtagggcgc
     1921 cgggtggtcg ccgacgaggc tggcggcctg ctcgaagttc tcgatggccg actcgtagtg
     1981 gccctcctgt aggtcggcca tcgcgaggcc gaagtaggct tcggcgaggc cgcgccactc
     2041 gccgttgcgg cggtagtgtt cgagcgcctt gctgatgtgg tcgcgcgcga tggtgtgctc
     2101 gttgatggag cggtagacgc gcgcgagcgc cgcgtagatg gcgccgagct gcgcgtccgt
     2161 gccgtggcgc tcgaccgcct cgcgcagctc cgccgagagc atgccgacgg ccttcgggtg
     2221 gtcgcccgcg tagttgtagg cgatgccgat ctgcacgcgc agggccgaga aggtctcggc
     2281 gtcgagcgtg ccgcgcgcct ccgccggctc gtacatccgc acgacttcga gcgactcttt
     2341 gtagcggccc aggatttcga gggcgatgga gagcgtgcgg cgcgactgcg cgaggagttg
     2401 cgggtcgcgg cgcgcggcgt tgatgagctg gtggaggcgc tgctcggcgg cgctcggcag
     2461 gccgcggtcg agcagttcgc gcgtctctgc gagttcgcgg tagaggttgg cggtcgtgtc
     2521 cgtggcgagg ctcgccaccg tgcggcgcgc gtccgcgagg ttgaccacgc cggggcgcgt
     2581 ctctcccctc gtgttcgggc cggactccgc gccctctttc ttgaaacgcg atgtcatgtc
     2641 agcaggctgc cactccaata gaaagtggtg agtagggcgc ttcttctttt tgaaagtttt
     2701 agaacggtgt ggaagaagga cgcttcggcc tctcgatgca tccctcaaaa cagtcccaaa
     2761 ggcttgtaca ttccagacat tagcacagaa aacattgagc cgtaaacgga agagttcggt
     2821 tgcaacgaaa tgagcagtga acggtaacag acagccttag
//
LOCUS       DBNGOH010000403         5831 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1528295, whole genome shotgun sequence.
ACCESSION   DBNGOH010000403 DBNGOH010000000
VERSION     DBNGOH010000403.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5831)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5831)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5831
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1528295"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            222..1394
                     /gene="wecB"
                     /locus_tag="AC159P_07960"
     CDS             222..1394
                     /gene="wecB"
                     /locus_tag="AC159P_07960"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002630717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-hydrolyzing UDP-N-acetylglucosamine
                     2-epimerase"
                     /protein_id="HIR3695426.1"
                     /translation="MLKVINVVGARPNFMKVAPIVEAMRRRASEFAPLVVHTGQHYDE
                     RMSDAFFRDLGLPRPDVHLGVGSGTHARQTAAVMERFEPVVLEERPDWVVVVGDVNST
                     LACALVCAKLLVPVAHVEAGLRSRDRTMPEEINRLVTDQLSDLLLTPSVDADANLRAE
                     GIPDERIRFVGNVMIDSLLKQLERAASSNARADLGVEGRDYAVVTLHRPSNVDERVTL
                     ARIVSALEEIGARLPVVFPIHPRTRKNLAEFGLLEEVERRGRVRLVEPLGYLDFLRLY
                     SGARLVLTDSGGIQEETTALGIPCLTLRENTERPVTVELGTNRVVGTDPARIIGAAQE
                     ALEGGAGRCARRVPPLWDGHAADRILDALLAETPRGAGVNARVSPPAYAGLHGEKI"
     gene            1538..3088
                     /locus_tag="AC159P_07965"
     CDS             1538..3088
                     /locus_tag="AC159P_07965"
                     /inference="COORDINATES: protein motif:HMM:NF016800.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008754 - O-antigen ligase activity
                     [Evidence IEA];
                     GO_function: GO:0008755 - O antigen polymerase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HIR3695427.1"
                     /translation="MGEARDLQELNPSPRKLPPGRLDMSNDHSLLDYDPVVPARPRRT
                     DAGEPAWDETRPPRDLPHDVAEDAGRGQARRNVARPEAVSAGGRLLRKGHVVSYVGVF
                     LFTVLLLFRPYELIPALSFLSSAAYWMAIATLLVYLPSQFALEGNLSAGLREVFLILL
                     LCLAGLLSIPFARNSVEAWDAFNDTFIKAVLIFIVMVNVVRTEWRLLGLIYLSLGVTV
                     VLSIAAVNDYRAGNFVVEGYRIKGLIGGMFGNPNDLALHLVTMTPIFVALMLGSRNLP
                     GKLIFGAGAVLSVAANVVTYSRAGFLGLLGMLVVLLWKLGRGKRFRVSVVGAACLSLF
                     IALSPGSYGVRVLSIFIPGLDPVGSSSNRRGILDRSVLVSLRNPLTGIGMGNFHIVSL
                     GETVSHNAYTQVSAEMGIAALVLYLMLITAALRRLKRIELATYAPRRRPRYFYLAVGL
                     QASIVGYMISSFFASVAYQWYVYYLVAYAVCLSRLHAAQTAAAGGEASAAGAAPADTE
                     AARVGAGA"
     gene            3100..4290
                     /locus_tag="AC159P_07970"
     CDS             3100..4290
                     /locus_tag="AC159P_07970"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR3695428.1"
                     /translation="MELAALIIFWLCAAALVYTYAGYPLLLVVLGRWRGREVLRRDWT
                     PSVSVIITAYNEERDLAAKLENTLALDYPAEKLEVMVASDCSDDRTDEIARSFAPRGV
                     RLHRQMARLGKTAAQNAAVERARGEILLFSDATTLYRPDVLRVIVRNFADPTVGCAAG
                     RLIYIDPERSTVGRGARSYWDYEVFLKERESRVCSLIGVSGCLYAVRRSAYVPLYHEA
                     CSDFLIATKMVEQGLRAVYEPGAVCTEETNSRAHRELRMRVRVITQTYTDLWRHRAML
                     NPLRSGFYAVQLFSHKVMRYLVPVFLCGLLAASALLAAGSAFYLLALAGQLGCYAAAG
                     VGWLLERTGRKAGPLAFPLYFVLANLAALVAFYQFLRGERYARWEPTREPAPLTLDRP
                     PHGN"
     gene            4344..5300
                     /locus_tag="AC159P_07975"
     CDS             4344..5300
                     /locus_tag="AC159P_07975"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR3695429.1"
                     /translation="MSVIIPAYNAAAYIGGALESVFAQTFEDYEVIVVNDGSPDTPAL
                     ERALAPYAGRIAYVEQENRGPSGARNAAIEAARGHYVALLDSDDEWLPEYLAEQVGMF
                     EADPRLDMVYADAELFGDSSLAGKTFMESAPSRGPVTFESLLRYESSIITSGTVARKQ
                     ALVEAGLFDEAFVRCEDFDLWLRLAHRGGRISYQRRVLARHRAHAGSLAADPILMVEA
                     QIRVLEKSLHLQPLTAAQRELIERQRLNCSAQIDLERGRRHFAGGEYEQASAALRRAN
                     DFYRSRRLRLVLWALRAAPGVLSSVARARQKLSGRGAASGAR"
     gene            5309..>5831
                     /locus_tag="AC159P_07980"
     CDS             5309..>5831
                     /locus_tag="AC159P_07980"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR3695430.1"
                     /translation="MATPTYHIAHVLPWPAVGGTEHATLRIARALEGEQFRSVAFCPG
                     DESPVRGMFAAGGLDTAAYEGVEPSYRHPAAFLRSSLRLAREFRRRRVALVHCSDLLA
                     AYYAAVAGKMAGARVLCHIRCSYPQVARRHRHFLRAVDRFVFVSQDAWRSFGHAVPGH
                     LGRVVYDGVATGGG"
BASE COUNT          804 a         2015 c         2062 g          950 t
ORIGIN      
        1 cgtggtgatc gtggcggatc acagcggggt ggactaccgc agggtgtgcg gggaggcgcg
       61 gctggtggtg gacacgcgga acgcggtggg cggggaggtg cgccgctcca gccgcgctcg
      121 cgtcatcaga ctgtgaggct ggggttcgtg accaacgggc cgaggccgcg aacgccggcc
      181 cgccgggagg cgacgacgga taaccgattg ctcttcgccg aatgctgaaa gtcatcaacg
      241 tagtgggggc gcggcccaac ttcatgaagg tcgcgccgat cgtggaggcg atgcggcggc
      301 gcgcgtcgga gttcgcgccg ctcgtcgtcc atacggggca gcattacgac gagcgcatgt
      361 ccgacgcctt ctttcgcgac ctcggcctgc cccgcccgga cgtgcacctc ggcgtcgggt
      421 cggggacgca cgcgcggcag accgccgccg tgatggagcg gttcgagccg gtcgtgctcg
      481 aagagcgacc ggactgggtg gtggtcgtcg gcgacgtgaa ctcgacgttg gcgtgcgcgc
      541 tcgtgtgcgc gaagctgctg gtgccggtgg cgcacgtcga ggcgggcctg cgcagccgcg
      601 accgcacgat gcccgaggag atcaaccgcc tcgtcaccga ccagctcagc gatctcctgc
      661 tcacgccctc ggtggacgcg gacgcgaacc tgcgagccga gggcatcccc gacgagcgca
      721 tccgcttcgt cggcaacgtg atgatcgact cgctgttgaa gcagctcgaa cgcgccgcct
      781 cgtccaacgc ccgcgccgac ctcggcgtcg agggcaggga ctacgccgtc gtcacgctgc
      841 accggccttc gaacgtggac gagcgcgtga cgctcgcgcg catcgtctcg gcgctcgaag
      901 agatcggcgc gaggctgccg gtcgtcttcc ccatccaccc gcgcacgcgc aagaacctcg
      961 ccgagttcgg gctgctcgaa gaggtcgaac gccgtgggcg cgtgcggctc gtcgagccgc
     1021 tgggctacct cgacttcctg cgtctctaca gcggggcgcg gctggtgctg acggactcgg
     1081 gcggcataca ggaggagacg acggcgctcg gcatcccgtg cctgacgctg cgcgagaaca
     1141 cggagcggcc cgtcacggtc gagctgggga cgaaccgcgt ggtcggcacc gacccggcga
     1201 ggatcatcgg tgcggcgcag gaggcgctgg aaggcggggc ggggcggtgt gcgcggcgtg
     1261 tgccgccgct gtgggacggc cacgccgcgg atcgcatcct cgacgccctg ctggccgaaa
     1321 cgccgcgcgg ggcgggcgtc aacgcgcggg tcagtccgcc ggcttacgcg gggttacacg
     1381 gggaaaagat ttgaaaggtc ttttaccggt gcgggcattg gagcggacat ccgtccgaaa
     1441 gcaagcctgt ctccgggaag tccggggtgg gagggtgtcg agctgcgcgg gataaaggct
     1501 gcgtagacgg tcgggcgcgg tcgcacagga cgcctctttg ggcgaggcgc gtgacctcca
     1561 ggagttgaac ccatccccgc ggaaactgcc gccggggcgg ttggacatgt cgaacgacca
     1621 ttcacttctc gattacgatc ccgtcgtccc tgcgcgtccg agaaggacgg acgcgggcga
     1681 accggcgtgg gacgagaccc gtccgccgcg cgacctgccg cacgacgttg cggaggacgc
     1741 gggccggggg caggcccggc ggaacgtcgc ccgacccgag gccgtgtcgg ctggggggcg
     1801 cctgctccgg aaggggcacg tggtgagcta tgtcggcgtg ttcctgttca cggtgttgct
     1861 tctcttcagg ccctacgaac tcatcccggc cctctccttc ctctcgtcgg cggcgtattg
     1921 gatggcgatc gccacgctgc tcgtctacct cccttcgcag ttcgccctgg agggcaacct
     1981 ctccgccggc ctgcgcgagg tctttctcat cctgctcctg tgtctcgccg ggctgctctc
     2041 catcccgttc gcgcgcaaca gcgtcgaggc ctgggacgcc ttcaacgaca cgttcatcaa
     2101 ggccgtcctg atcttcatcg tgatggttaa cgtcgtccgc accgagtggc ggctgctggg
     2161 gttgatctac ctgtcgctgg gcgtcaccgt cgtcctcagc atcgccgccg tcaacgacta
     2221 ccgcgcgggc aacttcgtgg tcgaagggta tcgcatcaag ggcctgatcg gcggcatgtt
     2281 cggcaacccg aacgacctgg cgctccacct cgtgacgatg acgccgatct tcgtggccct
     2341 gatgctcggc tcgcgtaacc tgcccggcaa actgatcttc ggcgcgggcg cggtgctctc
     2401 ggtggcggcc aacgtcgtca cctactcgcg ggccggcttc ctcgggctgc tcgggatgtt
     2461 ggtcgtcctg ctgtggaagc tcgggcgggg caagcgcttc agggtctccg tggtgggggc
     2521 cgcctgcctg tcgctcttca tcgcgctctc gcccggcagc tacggcgtcc gcgtcctctc
     2581 gatcttcatc ccgggactcg accccgtcgg ctcgtcgtcc aaccggcggg gcatcctcga
     2641 ccgctcggtg ctggtctctc tgaggaatcc cctgaccggc atcgggatgg gcaacttcca
     2701 catcgtctcg ctcggcgaga cggtctcgca caacgcctac acgcaggtct ccgccgagat
     2761 gggcatcgcg gctctggtgc tctacctgat gttgattacg gcggcgctga ggcggctcaa
     2821 gcggatcgag ctggcgacgt acgccccgcg ccggaggccg cgctactttt atctcgccgt
     2881 ggggctacag gccagtatcg tcggctacat gatcagtagc tttttcgcgt cggtcgccta
     2941 ccagtggtac gtctactatc tggtcgcgta cgccgtctgc ctgagccgcc tccacgccgc
     3001 gcagactgcc gcggcgggcg gcgaggcgtc ggctgcgggg gctgccccgg cggacacgga
     3061 ggcggcgcgg gtcggcgccg gcgcgtagat ggaggttcgg tggagctagc tgctctgatc
     3121 atcttctggc tgtgtgcggc ggcgctcgtc tacacctacg ccggctaccc gctgctgctg
     3181 gtcgtcctcg gacgctggcg ggggcgtgag gtgctgcgcc gggactggac gcccagcgtg
     3241 agcgtgatca tcacggccta caacgaggag cgcgacctgg cggccaagct ggagaacacg
     3301 ctggcgctcg attatcccgc ggagaagctg gaggtgatgg tcgcctcgga ctgctccgac
     3361 gaccggacgg acgagatcgc gcgctcgttc gcgccccgcg gcgtccggct ccaccgccag
     3421 atggcgcgcc tcggcaagac cgccgcgcag aacgcggcgg tcgagcgtgc gcggggcgag
     3481 atcctgctct tctccgacgc caccacgctc taccggcccg acgtgctgcg ggtcatcgtg
     3541 cgcaacttcg ccgaccccac ggtaggctgc gcggctggcc ggctcatcta catcgacccg
     3601 gagcgctcga cggtcgggcg cggggcgcgc tcctactggg actacgaggt cttcctcaaa
     3661 gagcgcgaga gccgcgtctg ctccctgatc ggcgtctcgg gctgtctcta cgcggtgcgc
     3721 cgctcggcct acgtcccgct ctaccacgag gcgtgcagcg acttcctgat cgcgaccaag
     3781 atggtcgagc aaggcctgcg cgcggtctac gagccggggg cggtctgcac ggaggagacc
     3841 aacagccgcg cacacaggga actgcgcatg cgcgtgcgcg tcatcacgca gacctacacc
     3901 gacctgtggc gccaccgcgc gatgttgaac ccgctgcgca gcgggttcta cgccgtgcaa
     3961 ctcttctcgc acaaggtcat gcgctacctc gtgcccgtct tcctgtgcgg gctgctggcg
     4021 gcgtccgcgc tgctggcagc cggctccgcc ttctacctct tggccctcgc gggccaactc
     4081 ggctgctacg cggcggcggg ggtggggtgg ctgctcgaac gcacggggag gaaggcgggg
     4141 ccgctcgcct tcccgctcta tttcgtgctc gccaacctcg ccgcgctggt cgccttctac
     4201 cagttcctgc gcggcgaacg ttacgcgcgc tgggagccga cgcgcgagcc ggcccccctg
     4261 accctcgacc gaccgccgca cggaaactga tggcgaccga cgcgacaacg cccgagtcgg
     4321 ggcgaacagg tggccctccg ctggtgagcg tcatcatccc cgcctacaac gccgccgcct
     4381 acatcggcgg ggcgctggag tccgtcttcg cgcagacctt cgaagactac gaagtgatcg
     4441 tcgtcaacga cggctcgccc gacacgcccg cgctcgaacg cgcgctggca ccctacgccg
     4501 ggcgcatcgc ctacgtcgag caggagaacc gcggcccgag cggggcgcgc aacgcggcca
     4561 tcgaggcggc gcgcggccac tacgtcgcgc tgctcgacag cgacgacgaa tggctgcccg
     4621 agtacctggc cgagcaggtc gggatgttcg aggccgaccc gcggctcgac atggtctacg
     4681 cggatgccga gctgttcggc gactcgtcgc tggccgggaa gacgttcatg gagagcgcgc
     4741 cctcacgcgg ccccgtcacg ttcgagagcc ttttgcgtta cgagtcttcg atcatcacct
     4801 cgggcacggt cgcgcgcaag caggcgctcg tcgaggcggg cctcttcgac gaggctttcg
     4861 tccgctgcga ggacttcgac ctctggcttc gcctcgccca ccgcggcggg cgcatctcgt
     4921 accagcggcg ggtgctggcg cgacaccgcg cgcacgccgg gagcctggcc gccgacccca
     4981 tcctgatggt cgaggcccag attcgggtgc tggagaaatc cctgcacctc cagccgctga
     5041 ccgcggcgca gcgcgagttg atcgagcggc agcggttgaa ctgctcggcg cagatcgatc
     5101 ttgagcgcgg cagacgccac ttcgcgggcg gcgagtacga gcaggcgtcc gcggcgctgc
     5161 ggcgcgccaa cgacttttac cggagccgcc ggctgcggct cgtgctctgg gccttgcgcg
     5221 ccgcccctgg ggtcttgagt tccgtcgccc gcgcgcggca gaagttatcc gggagggggg
     5281 ccgcctccgg ggcgaggtaa gcgaaggcat ggcgaccccg acataccaca tcgcgcacgt
     5341 gctgccctgg cccgccgtcg gcggcaccga gcacgccaca ctgcgcatcg cgcgggcgct
     5401 ggagggcgag cagttccgaa gcgtcgcctt ctgccccggc gacgaaagcc ccgtgcgcgg
     5461 gatgttcgcc gcgggcggtc tcgacaccgc cgcttacgag ggggtcgagc cgagctaccg
     5521 acaccccgcg gcgttcctgc gctccagcct ccgcctcgcg cgggagttca ggcggcggcg
     5581 ggtcgccctc gttcactgct cggaccttct ggccgcctac tacgcggctg tcgcggggaa
     5641 gatggcaggg gcgcgcgtcc tgtgccacat ccgctgcagc tacccgcagg tcgcgcggcg
     5701 gcaccgacac ttcctgcgcg ccgtagaccg gttcgtcttc gtctcgcaag acgcgtggcg
     5761 gagtttcggc cacgcggtgc cggggcactt gggccgggtc gtctacgacg gcgtcgcgac
     5821 cggcggcggg g
//
LOCUS       DBNGOH010000404         2517 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2627876, whole genome shotgun sequence.
ACCESSION   DBNGOH010000404 DBNGOH010000000
VERSION     DBNGOH010000404.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2517)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2517)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2517
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2627876"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1707)
                     /locus_tag="AC159P_07985"
     CDS             complement(<1..1707)
                     /locus_tag="AC159P_07985"
                     /inference="COORDINATES: protein motif:HMM:NF014214.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M36 family metallopeptidase"
                     /protein_id="HIR3695431.1"
                     /translation="MKRRTGWALLCAASLILVSLAWDFAPRVTSQGRQRGAGAGDKPQ
                     RYATFENFDIRSQDSKPAQLAQERRQAARRTKAKADTGVRGEAMELAGQRLAARVPGA
                     RVADNERTGVPEVVTATARGQFLTARSNQSPEAVVRGFLSRNAELYGLSARQAASLKK
                     TADYANPAGNLRWVVLEQEIGGIPVFQGQLVAALTAEGELVRTVSGLAAGVDDTAGSK
                     AGGKGANSSHALSAKPGLSAAEAVSAAAASIGMSVDPGTLAIRETSEDGSTVVFHPGP
                     FADEVKAELRFFQETPGEITLAWSMVLWQDMPAYYTLVDAEDGELLWRKNLLNEQTQP
                     ATYVAYGSDSPAPLSPNLNLLTPTVPTAQGAAVGRDTFTLISEHPLGDPWLNDGDNTT
                     TGNNVDSGMDLVAPDGIDAGSRPVGAPLRVFDFPYNPAPGIPPPGDAPTLANYRFGEA
                     VNMFFWTNRYHDRLYALGFTEAARNFQQNNFGRGGLGNDRVLAQGQDFSGTDNANFLT
                     PPDGTSGRMQMYIFTGPTPDRTSGLDQEILIHELTHGTSNRLHANAAGLATTMSGGMG
                     EGW"
BASE COUNT          354 a          827 c          898 g          438 t
ORIGIN      
        1 ccagccctcg cccatgccgc cgctcatcgt cgtggcgagg cccgcggcgt tggcgtgcag
       61 gcggttcgag gtgccgtgcg tcagctcatg gatgagaatc tcttggtcga ggcccgacgt
      121 gcgatccggc gtcggcccgg tgaagatgta catctgcatg cggcccgagg tgccgtccgg
      181 gggcgtcagg aagttggcgt tgtcggtgcc cgaaaagtcc tgcccctgtg ccagcacgcg
      241 gtcgttgccg aggccgccgc gcccgaagtt gttctgctgg aagttgcgcg ccgcctctgt
      301 aaagccgagc gcgtagagcc ggtcgtggta gcggttcgtc cagaagaaca tgttgacggc
      361 ctcgccgaag cggtagttgg cgagcgtcgg cgcgtcgccc ggcgggggga tgcccggcgc
      421 cgggttgtag gggaagtcga agacgcgcag gggcgcgccg acggggcggc tgcccgcgtc
      481 gatgccgtcg ggcgcgacca agtccatgcc cgaatcgacg ttgttgccgg tcgtcgtgtt
      541 gtcgccgtcg ttgagccacg ggtcgcccaa ggggtgctcg ctgatgagcg tgaaggtgtc
      601 acgcccgaca gccgcgccct gcgcggtcgg gacggtgggg gtcagcaggt tgagattcgg
      661 cgagagcggg gccgggctgt cgctgccgta ggcgacgtag gtcgccggct gcgtctgctc
      721 gttgagcagg ttcttgcgcc agagcagctc gccgtcctcc gcgtcaacca gcgtgtagta
      781 ggccggcatg tcctgccaga gcaccatcga ccaggcgagc gtgatctcgc cgggggtctc
      841 ctggaaaaag cgcagctcgg ccttcacctc gtcggcgaag gggccggggt ggaagacgac
      901 cgtgctgccg tcttcggaag tctccctgat ggcgagtgtg ccgggatcga cgctcatgcc
      961 gatggaggcc gccgccgcgg agaccgcctc ggcggcgctc aggccgggct tggccgagag
     1021 cgcgtggctg gagttggcgc ccttgccgcc ggctttcgac cccgctgtgt cgtcaacgcc
     1081 cgcggcgagg ccgctgacgg ttctgacgag ttcgccctcc gcggtcagcg ccgccacgag
     1141 ctggccctgg aagaccggga tgccgccgat ctcctgctcc agcacgaccc agcgcaggtt
     1201 gccggcgggg ttggcgtagt cggcggtctt cttcaggctg gccgcctgcc gcgcgctcag
     1261 gccgtagagt tcggcgttgc gcgagaggaa gccgcgcacg accgcctcgg gtgactggtt
     1321 cgagcgcgcg gtgaggaact gcccgcgcgc cgtggccgtg accacctcgg gcactcccgt
     1381 ccgctcgttg tccgcgaccc gcgcgccggg gacgcgcgcg gccaggcgtt ggccggccag
     1441 ctccatcgct tcgccgcgga cgcccgtgtc ggccttcgcc ttcgtgcgcc gtgccgcctg
     1501 cctgcgctcc tgcgccaact gggccggctt ggagtcttgg ctcctgatgt cgaagttctc
     1561 gaacgtggca taacgctggg gcttgtcacc cgcaccggcg ccgcgttgcc tgccttggct
     1621 cgtcacgcgc ggcgcgaaat cccacgcgag cgaaactaag atgagggacg cggcacaaag
     1681 cagcgcccat cccgtacgtc ttttcatatg tggttctcct cggcttgggg atacagcttc
     1741 cggccactgc ttgcgaggaa ggggccggca ggagttaacg tcttaagaac taagctggag
     1801 cttgcgtttc gtccgcacga tatcggcggc gtcgggtgac gcccggcagt cttctaaaga
     1861 ccgtgaggat gatgtacagc gaggtcgtgg gacgccggaa gggacggttc cggcctacgc
     1921 gtttaccggc gagatagcat ggagtgcccg gctactggac gcgtaccgcc cgcgcgagga
     1981 gtgcgggccg ccttcttttt caaacgtgta gggcgcgcac actatacttt cccgcggcgc
     2041 gtgtcaacca aaaatatgta cggaatcgac aaaattggac gcggcggatg gcgggcggcg
     2101 ggtcgcgcgc ggcggcggtc gggccgcccc cgcgcgcctc cgggggggcg gtctcggtga
     2161 gcgactcgcc acgcgccgtt cgccgcccaa gacttcattg cgcgcgcgta cgcttggtgc
     2221 taatctctcg cgcgtaacgg cttggatttc gctcctctag cacgcagcac agttcgcacg
     2281 attttcgccg gccccggcgg cgccgtctcc gcggcagcga cagccggagc cggcgctctc
     2341 ccgtccgcac ccgtgtgatc gttggaaggg ccacggacga ccggaagcac accaacattt
     2401 tacggaggac gtaaatgatc gagcactcct tgcaactgcc gctcagtctc ggcctgttcc
     2461 aggacggcgg catcttccac gcgcccgacg gggccgacct gacgcgcatc atcctgc
//
LOCUS       DBNGOH010000405         3564 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_245227, whole genome shotgun sequence.
ACCESSION   DBNGOH010000405 DBNGOH010000000
VERSION     DBNGOH010000405.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3564)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3564)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3564
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_245227"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(205..1557)
                     /locus_tag="AC159P_07990"
     CDS             complement(205..1557)
                     /locus_tag="AC159P_07990"
                     /inference="COORDINATES: protein motif:HMM:NF013799.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HIR3695432.1"
                     /translation="MIDTCSSHNPAAALLEPEEAAARPASKTVETRPRRLLLCLDGVP
                     LEVIKEAKARGLFDNFREPARLLSPFPTVTNVALAEMLKATPPQGYESLYFDRDERRL
                     AGGVRKYLGRRTPDKIPSSYMDELDYQEPLHFEFLIYVATDAVWRADMRRFRERFRAA
                     PAGRDFFAFLKATDGLLHRRGPRHLQVALESLDRILLDVQAACGAGTEIVMFSDHGMN
                     LEENRRVHLQTFLKRGGFEVVGEMRGRVREGRRPRVSAPAFGLCGYAALYCGEESDAG
                     EVAGALGALEGVDFSVRRDEEGSGVIVEGSRGRARIRRREDEAGACSYAYEQLAGDPL
                     RLASVARAIEAEGLTDAEGFAPDAAWLARTAEHEYPDALANLHGSLHAPRVRQTADVL
                     VSLRDGYYYGATAFTHMIRLLATHGNALRPSTTAFLMSTHRDFPAHVRAADARPFLKE
                     "
     gene            complement(1685..3034)
                     /locus_tag="AC159P_07995"
     CDS             complement(1685..3034)
                     /locus_tag="AC159P_07995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695433.1"
                     /translation="MIVVKLARHLSVMLSILVAATSAASAQPAKAPAAESAPAAFDQK
                     RFEALRAEGFEALYSLEYEKARLRFKEIARLFPEHPAGPQFLAASLWLKTLNESRRLQ
                     ASLYNTEGFYKEKDDKPDAKMVAEFRELTRQAKALSEARLKRDPKDIEALYYLGATEG
                     LKAAFGAMVERSWYSSLRNGGDAVDRHRAVVKLDPNYHDARITIGMYDYVTGSLPLPF
                     KLAAAIGGVRGSKKKGLASIEQAAREARWAQDDAKVLLIALYKREKRYLDAYGVANEL
                     GAKYPRNYLFKMEAADALVTHAAEVRATDPAAAKKSEGEAFAIFESLVRGAEAKGGPR
                     APLDLVQYTYGEALFVAGEADRAAKAFLSSSAAPNAEASMVTRSKLRAAQALDVAGKR
                     EEALTQYKAVLARPNLYDSHEDAKRGLKEPYKTPRKVADATADSDGDADATKTAQPD"
     gene            complement(3221..>3564)
                     /locus_tag="AC159P_08000"
     CDS             complement(3221..>3564)
                     /locus_tag="AC159P_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HIR3695434.1"
                     /translation="IVRAFEEGKRFADEFPNARTTASGLRVPKAVGDFLILDALRASG
                     GTAIAVTDEELIAAAKEIGAAEGLFVAPEGAACLPALRKLVERGEVSREETVVLFNTG
                     AGVKYLESFGG"
BASE COUNT          536 a         1233 c         1189 g          606 t
ORIGIN      
        1 tgtaagcgca cgcgccgcaa gaagcaagcg ggaaaggcag taggcggtag gcggtaggca
       61 gtaggcagtc aaagcaagag gcttgagaga ttgaatctca agccccttgc tttatcgctc
      121 gaagagcaaa gacgcttcag gactttacct ctcaggcatc ttcgctcttc atgtcttgac
      181 tgcctactgc ctaccgccta ccgtctactc tttcagaaac ggtcgcgcat cggcggcgcg
      241 gacgtgtgcg gggaagtcgc ggtgggtgct catgaggaag gcggtggtgc tggggcggag
      301 cgcgttgccg tgcgtggcga gcaggcggat catgtgtgtg aaggccgtcg cgccgtagta
      361 gtagccgtcg cgcagggaga cgagcacgtc cgccgtctgc cgcacgcgcg gcgcgtggag
      421 cgagccgtgg aggttcgcga gcgcgtccgg gtattcgtgc tccgccgtgc gcgcgagcca
      481 cgcggcgtcg ggcgcgaacc cttcggcgtc cgtcaagcct tccgcttcga tggcgcgcgc
      541 gaccgatgcg agccgcaacg ggtcgcccgc cagttgctcg taggcgtagg agcaggcgcc
      601 cgcttcgtcc tcacgtcggc ggatgcgcgc gcgcccgcgc gagccttcga cgatgacgcc
      661 gctcccttct tcgtcgcggc gcacgctgaa gtccacgcct tcgagcgcgc cgagcgcgcc
      721 cgccacctcg cccgcgtccg actcctcgcc gcagtagagc gccgcgtagc cgcacagccc
      781 gaaggcgggc gccgagacgc gcgggcgccg cccctcacgc acgcgaccgc gcatctcgcc
      841 gacgacctcg aagccgcccc gcttcaggaa ggtttgcagg tggacgcgcc ggttctcttc
      901 gaggttcatg ccgtggtcgg agaacatcac gatctcggtg ccggcgccgc acgccgcctg
      961 cacgtccagg aggatgcggt cgagcgattc gagcgcgacc tgcaagtggc gcggcccccg
     1021 ccggtgcagc agcccgtcgg tcgccttgag aaaggcgaag aagtctcggc ccgcgggcgc
     1081 ggcgcggaag cgctcgcgga agcggcgcat gtccgcccgc cacacggcgt cggtcgcgac
     1141 gtagatcagg aactcgaagt gcagcggctc ctgatagtcc agctcgtcca tgtaggacga
     1201 gggaatcttg tcgggcgtgc gccgaccgag gtacttgcgc acgccgcccg cgaggcgccg
     1261 ctcgtcgcgg tcgaagtaga ggctctcgta cccttgcggc ggcgtcgcct tcagcatctc
     1321 ggcgagcgcg acgttggtca cggtcgggaa cggcgacagg agccgcgccg gctcgcggaa
     1381 gttgtcgaac agcccgcgcg ccttcgcctc tttgatgacc tccagcggta cgccgtccag
     1441 acagagcagc aggcggcgcg ggcgcgtctc gaccgtcttc gacgcgggcc gcgccgccgc
     1501 ctcttcgggt tcgagcagcg ccgccgccgg attgtgcgag gaacatgtat caatcattgg
     1561 agtagccaga agccggaagt cggaagtcgg aagtcggaag aaaaactttc cacttcaaag
     1621 cagtagtgat gtcagcgcca gggtcggcag cgcggagcga tgctgacttc cgacttccga
     1681 ctcctcagtc gggctgcgcc gtcttcgtcg cgtcggcgtc cccgtcgctg tcggcggtgg
     1741 cgtcggcgac cttgcgcggc gtcttgtacg gctccttgag gccgcgcttg gcgtcctcgt
     1801 gcgagtcgta gaggttgggg cgcgcgagca cggccttgta ctgcgtcagc gcctcctcgc
     1861 gcttgcccgc cacgtcgagc gcctgcgcgg cgcgcagctt cgagcgcgtc accatgctcg
     1921 cctcggcgtt cggcgccgcc gaagacgaga ggaacgcttt ggcggcgcgg tcggcctcgc
     1981 ccgcgacgaa cagcgcctcg ccgtaggtgt actgcacgag gtcgagcggc gcgcgcgggc
     2041 cgcccttcgc ctcggcgccg cgcacgagcg attcgaagat ggcgaacgcc tcgccttcgg
     2101 acttctttgc cgccgccggg tcggtcgcgc gcacctccgc cgcgtgcgtg acgagcgcgt
     2161 ccgccgcctc catcttgaac aggtagttgc gcgggtactt cgcgcccagc tcgttcgcca
     2221 ccccgtaggc gtcgaggtaa cgcttctcgc gcttgtagag ggcgatcagc agcaccttcg
     2281 cgtcatcctg cgcccagcgc gcctcgcgcg ccgcctgctc gatggaggcc agccccttct
     2341 tcttcgagcc gcgcacgccg ccgatggccg ccgccagctt gaagggcaga gggagcgagc
     2401 cggtcacgta gtcgtacatg ccgatggtga tgcgcgcgtc gtggtagttc gggtcgagct
     2461 tgacgacggc gcggtggcgg tcaaccgcgt cgccgccgtt gcgcagggaa gagtaccaac
     2521 tgcgctcgac catcgcgccg aaggcggcct tcagcccctc ggtcgcgccg agataataga
     2581 gcgcctcgat gtccttcggg tcgcgcttca ggcgcgcttc ggagagcgct ttggcctgcc
     2641 gcgtcagctc gcggaactcg gccaccatct tcgcgtccgg cttgtcgtcc ttctctttgt
     2701 aaaagccttc cgtgttgtaa agcgacgcct gcaagcgccg cgactcgttc agcgtcttca
     2761 gccacaggct cgcggcgagg aactgcggcc ccgccgggtg ttcggggaag agccgcgcga
     2821 tctccttgaa gcgcaaccgc gccttctcgt attcgaggct gtagagcgcc tcgaagcctt
     2881 cggcgcgcag cgcctcgaat cgcttctggt cgaacgccgc gggcgcactc tccgccgcgg
     2941 gcgccttcgc cggctgcgcc gaggcggcgg acgttgccgc gacgagtatc gacaacatga
     3001 ctgacagatg acgagcgagc ttcacgacaa tcatctctcg aaccctttct ttatgactca
     3061 gccactcttc ccgcaaaacc cgaccaccct tcccgccgac gagtcttcaa gtgtcatccc
     3121 atgccgaaga ggtgtgatcg tccaaatact tcgacgtatt tcctgacgcc gacggtgccc
     3181 ggcctgttaa ctttgatgaa gggcgcggcc accgccctcc tcaaccgccg aagctctcca
     3241 gatacttcac gcccgcgccc gtgttgaaga gcacgaccgt ttcctccctg ctcacttcgc
     3301 cgcgctcgac gagcttcctc agagcgggca ggcaggccgc gccttcgggc gcgacgaaca
     3361 agccttccgc cgcgccgatc tctttcgccg ccgcgatcaa ctcttcgtcg gtgacggcga
     3421 ttgccgtccc gcccgaagcg cgcagcgcgt cgaggatgag gaagtcgccg acggccttcg
     3481 gcacgcgcag gcccgaggcg gtcgtccgcg cgttcgggaa ctcgtccgcg aagcgcttcc
     3541 cttcctcgaa cgcgcggacg atgg
//
LOCUS       DBNGOH010000406         1729 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3545192, whole genome shotgun sequence.
ACCESSION   DBNGOH010000406 DBNGOH010000000
VERSION     DBNGOH010000406.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1729)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1729)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1729
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3545192"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            99..995
                     /locus_tag="AC159P_08005"
     CDS             99..995
                     /locus_tag="AC159P_08005"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR3695435.1"
                     /translation="MSENLSSLESSVRHGYAQVGDVRLHYAESGAGERLVVLLHGFPE
                     CWYSWRHQLAALGERFRVVAPDLRGYNLSDKPSEVKDYVIERLVDDVTGLIRHFGASE
                     AAIVGHDWGAAVAWAVAQRHPEYVWKLAALQVPPPAVWAKNITLKQLLRSWYMFFFQL
                     PALPEWFVSRDDFAAVEKMFRTTARPGTFTGTDIAVFKSALRQPGAVTGGINYYRANL
                     GARLRRAWERKPYAREELIRVPTLFVYGEQDMAIVPETVQGVGDYIDAPYEEIRLGRS
                     GHWVQQESPAEINAALLRFLTE"
     gene            1112..>1729
                     /locus_tag="AC159P_08010"
     CDS             1112..>1729
                     /locus_tag="AC159P_08010"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR3695436.1"
                     /translation="MDDSSPVIFNVEGAVARITLNRPEKRNALNDALISGLKEALRAA
                     DGEEAVRAVVIKGAGADFCSGADLSALQKISEGSISDNLEDARSLLELFVLIRRLRVP
                     VVAAVRGHALAGGCGLATACDIVLAAASARFGYPEVKIGFVPAMVMAILRRNVSEKRA
                     FELVTLGAETGAEEAAALGLVNRVFADDVFDEEVDKFVAGFGRVSR"
BASE COUNT          301 a          572 c          575 g          281 t
ORIGIN      
        1 gaccgacgcg cagggcaaga ccttctcgac ggcgctgctg cggatggtga tcgaagtgcc
       61 cgacgaagag gcgcgtactc tgaacctcgt ttaaagcgat gagcgaaaac ctttccagtc
      121 tcgaatcgtc cgtacgccac ggctacgcgc aggtcggcga cgtgcgattg cactatgcgg
      181 agagcggcgc gggcgaacgc ctcgtcgtcc tgctccacgg cttccccgaa tgctggtact
      241 cgtggcggca ccagctcgcc gcgctcggcg agcgcttccg cgtcgtcgcg cccgacctgc
      301 gcggctacaa cctgagcgac aagccttcgg aagtgaaaga ctacgtgatc gaacggctcg
      361 tggacgacgt gacagggttg atacgacact tcggcgcgag cgaggcggcc atcgtcggcc
      421 acgactgggg cgcggcggtc gcgtgggccg tcgcgcagag gcaccccgag tacgtctgga
      481 aactggccgc gctgcaagtg ccgccgcccg ccgtgtgggc gaagaacatc actctcaagc
      541 aactgctgag gagctggtac atgttcttct tccaactccc ggcgctgccc gaatggttcg
      601 tcagccgcga cgacttcgcc gccgtcgaga agatgttccg cacgacggcg cggcccggca
      661 ccttcacggg cacggacatc gccgtcttca agtccgcgct gcgccaaccc ggcgccgtca
      721 ccggcggcat caactactac cgcgccaacc tcggcgcgcg cctgcgccgc gcgtgggagc
      781 gcaagcccta cgcgcgcgaa gaactcatcc gcgtccccac gctcttcgtc tacggcgagc
      841 aggacatggc catcgtcccc gagacggtgc agggcgtcgg cgactacatc gacgcgccct
      901 acgaagagat acgactcggg cgcagcggcc actgggtgca acaggaatcg cccgccgaga
      961 tcaacgccgc gctcttgagg ttcctgaccg agtgagaagg cggggaggga aggcgctcta
     1021 cttttacctt cgcgcggacg cgaaagctcg taattcagtt tcgagcttct acatctgaaa
     1081 gcgaaactcc tttaagggac gcttcacttc tatggacgac agcagccctg tcatcttcaa
     1141 cgtcgaaggc gcggtcgcgc ggataaccct gaaccgtccg gagaagcgga acgccttgaa
     1201 cgacgcgctc atctccggac tcaaagaggc gctgcgcgcg gcggacggcg aagaggcggt
     1261 gcgggccgtc gtcatcaagg gcgcgggcgc ggacttctgt tcgggcgccg atctgtcggc
     1321 cttgcagaaa atctccgaag gctcgatcag cgacaacctc gaagacgcgc ggagcttgtt
     1381 ggagttgttc gtgctcatcc gtcggctccg cgtgccggtc gtcgccgccg tgcgcgggca
     1441 cgcgctggcc ggagggtgcg ggctggcgac ggcgtgcgac atcgtgctcg ccgcggcgtc
     1501 cgcgcgcttc ggctacccgg aggtgaagat cggcttcgtc ccggcgatgg tgatggcgat
     1561 tctgcgccgc aacgtctctg aaaagcgcgc gttcgaactc gtcacgctcg gcgccgagac
     1621 gggcgccgaa gaggccgccg cgctcggcct cgtcaaccgc gtcttcgccg acgacgtgtt
     1681 cgacgaagag gtggacaagt tcgtcgccgg cttcgggcgc gtgagccgc
//
LOCUS       DBNGOH010000407         2517 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2995857, whole genome shotgun sequence.
ACCESSION   DBNGOH010000407 DBNGOH010000000
VERSION     DBNGOH010000407.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2517)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2517)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2517
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2995857"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..262)
                     /locus_tag="AC159P_08015"
     CDS             complement(<1..262)
                     /locus_tag="AC159P_08015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695437.1"
                     /translation="MKLTARLLLLTALLTLTLARVPVARGVGQKEIGPNGSDAERLAR
                     SFGFAGFLARHHHIAFAGNLWRTVAAGRAGFGVLPGPARVLTA"
     gene            complement(482..955)
                     /locus_tag="AC159P_08020"
     CDS             complement(482..955)
                     /locus_tag="AC159P_08020"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3695438.1"
                     /translation="MGTIREYRDEDAPHLRACLVELQEYERRLDALLAEGEQMADRYI
                     EFILARCAETGGRVFVAEDEGRVVGFVSVWARVKSRAIEEREYEYAYVSDLVVLAAHR
                     GRGLGRALLRRAEEHARGEGATLLRIAVLAKNDGARRLYESFGFAERLLELAKTL"
     gene            complement(977..1678)
                     /locus_tag="AC159P_08025"
     CDS             complement(977..1678)
                     /locus_tag="AC159P_08025"
                     /EC_number="5.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047661 - amino-acid racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HIR3695439.1"
                     /translation="MKDITIGILAGMGPRSTAPFVDMLVTECRRQYGAKYDEEFPRMM
                     IYSLPVPFRADEPPDEGALRGVVCEGLRRLEATGVEFIAMPCNTVHVFHEELSKCVRV
                     PLLNMIDETLGAVPPAARRVALLATRMTSEAGLYQSGVARAGLCLVSPEGWQRRVDEL
                     IVTVKTSPDRAAAQGLWDGLVADAAEAGADTVLLGCTDLNAVDARVPAGVTLLDATAC
                     LARAVVRRYVERAAG"
     gene            complement(1821..>2517)
                     /locus_tag="AC159P_08030"
     CDS             complement(1821..>2517)
                     /locus_tag="AC159P_08030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695440.1"
                     /translation="EELIDLIAESGGKWVFIGMESIDPQNMADVNKNFSKPGEYGVVL
                     DRLARRDIYAITSFIFGMDNDTVGVADRTLREIRSWPPGLPVFGQLTPFPATPLYDRL
                     EKAGRLDRPKHWLDFAPFVMAHAPLKMTIDEAKAETRRAWEASYSPERNAQALEAISD
                     APVKYRISHLVARLFFRGIYFPQMTKRAWLGLLFQNRRAIYGLAREGASTWRAARKRQ
                     RAQLEALPRGSEQ"
BASE COUNT          396 a          866 c          852 g          403 t
ORIGIN      
        1 tcgcggtgag cacgcgcgcc ggaccgggca ggacaccgaa gccggcccgg cctgcggcga
       61 cggtgcgcca gaggttgccg gcgaacgcga tgtggtggtg tcgcgcgagg aagccggcga
      121 agccgaagga acgcgcgagc cgctcggcgt cggagccgtt cgggccgatc tctttctgcc
      181 cgacgccgcg cgcgacgggc acgcgcgcaa gcgtcagcgt cagcaacgcc gtcaacaaca
      241 acaggcgggc cgtgagtttc atcgagcttc tcctccgtaa gaccgaactt cgggcggggt
      301 tcttcgaacg tatgtgtcag gggagagtga gaagtttcaa gcattttacc ggcagaagcc
      361 cgaccgtgag ggagagcgcg gcggattcta tagggtcggc gtgcgtctgg caagattttt
      421 tccgacggga aggacgccca aacactttac ctctcgccgt cgggcgcggc gcgtgcgggg
      481 cctaaagagt cttggccagt tcgagcaggc gctctgcgaa gccgaagctc tcgtagaggc
      541 ggcgcgcgcc gtcgttcttc gccagcaccg cgatgcgcag gagcgtcgcg ccctcgccgc
      601 gcgcgtgctc ttccgcccgc cgcagcagcg cgcgcccgag gccgcggccc ctgtgcgcgg
      661 cgagcacgac gaggtcggag acgtacgcgt actcgtactc gcgctcctcg atggcgcggc
      721 tcttcacccg cgcccagacc gagacgaagc cgacgacgcg accctcgtct tccgcgacga
      781 acacgcgccc gcccgtctcc gcgcagcgcg cgaggatgaa ctcgatgtag cggtcggcca
      841 tctgctcgcc ttccgcgagg agcgcgtcca gccgccgctc gtactcttgc agctcgacca
      901 gacacgcgcg caggtgcggc gcgtcctcgt ccctgtattc gcggatcgtg cccatgcgtc
      961 cgttcacgcg gcggcgtcag cccgccgcac gctccacgta gcgccgcacg acggcgcgcg
     1021 ccaggcacgc ggtcgcgtcg agcagcgtca cccctgcggg cacgcgcgcg tcaaccgcgt
     1081 tgaggtctgt gcagccgagc agcaccgtgt cggcgcccgc ctccgccgcg tcggcgacga
     1141 ggccgtccca cagcccttgc gccgccgcgc ggtcgggcga ggtcttgacg gtcacgatca
     1201 gctcgtccac gcgccgctgc cacccttccg gcgagacgag acacagccct gcgcgcgcga
     1261 cgccgctttg atagaggccg gcctccgagg tcatccgcgt ggcgagcagc gcgaccctcc
     1321 gcgccgcggg cggcacggcg ccgagcgtct cgtcgatcat gttgaggagc ggaacccgca
     1381 cgcacttcga cagctcttcg tggaagacgt gcaccgtgtt gcacggcatg gcgatgaact
     1441 cgacgcccgt cgcttcgagc cgccgcaagc cctcgcagac gacgccgcgc agcgcccctt
     1501 cgtcgggcgg ctcgtcggcg cggaacggca cgggcagcga gtagatcatc atgcgcggga
     1561 actcctcgtc gtacttcgcc ccgtactgcc tccggcactc cgtcaccaac atgtccacga
     1621 acggcgccgt cgagcgcggc cccatccccg cgaggatgcc gatggtgatg tccttcatct
     1681 tcattaagtg agcagtgagc agtaagcggt gagcagtaag agaaacaagc cgggagcagc
     1741 aagacagaag aagaggcttg agactttacc tcccaagctt cattgctctt cttttgtttt
     1801 aactgctcgc tgctcaccgc tcactgctca ctgcctcggg gcagggcttc gagttgcgcg
     1861 cgttgccgct tgcgggcggc gcgccaggtg gaggctcctt cgcgcgcgag gccgtagatg
     1921 gcgcggcggt tctggaagag caggcccagc caggcgcgct tcgtcatctg cgggaagtag
     1981 atgccgcgga agaagaggcg cgcgacgagg tggctgatgc ggtacttgac gggcgcgtcg
     2041 ctgatcgctt cgagcgcctg cgcgttgcgc tcggggctgt agctcgcctc ccacgcgcgg
     2101 cgcgtctcgg ccttggcctc gtcaatcgtc atcttcaaag gcgcgtgcgc catcacgaag
     2161 ggcgcgaagt cgagccagtg cttcgggcgg tcgaggcgtc cggccttctc cagtctgtca
     2221 tagagcggcg tggccgggaa cggcgtgagc tgcccgaaga cgggcaaccc cggcggccac
     2281 gagcgaatct cgcgcaaggt gcggtcggcg acgcccaccg tgtcgttgtc catcccgaag
     2341 atgaaggagg tgatggcata gatgtcgcgc cgcgccagcc ggtcgagcac gacgccgtac
     2401 tcgcccggct tgctgaagtt cttgttcacg tcggccatgt tctgcgggtc gatcgactcc
     2461 atgccgatga agacccactt gccgcccgac tccgcgatca ggtcgatcaa ctcttcg
//
LOCUS       DBNGOH010000408         4090 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2628930, whole genome shotgun sequence.
ACCESSION   DBNGOH010000408 DBNGOH010000000
VERSION     DBNGOH010000408.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4090)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4090)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4090
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2628930"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            45..2828
                     /locus_tag="AC159P_08035"
     CDS             45..2828
                     /locus_tag="AC159P_08035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695441.1"
                     /translation="MTYIPISVSLVFLLLVSPFVTSCTSHAQATQTRPVGAAQSPRPE
                     YLWYEAENMSGLSTDERHEPRQNPSWRSPARAESPGWGINGPGVSAEWSQGGESEWNS
                     VAASADETKASIAQDLEAPREGRYRLWVRYADWVRRGEQFTARVVQDGREVFRHEFGA
                     ADLVDPNDEVSMYWGWAFAWGGSTEFSLKKGAARVSFEVERATEARRHVDCFVLTNDV
                     NYTPDGRRKPPFAAALALERWGATRATLAPLMSAPTAGDVVPERWKLRRLGGRDFQMP
                     WNIAEEFWKMYDQPAEARPVYPFNAEPIESFVGKYKGVREVPIFSSKLVVPVVYINHV
                     QEHLKEGAPFLRHLRETRTPFAILINYGAARFAEAAAGQEALKLLTGELREQFIGWIS
                     GESIGHVFTDVQQNLKLDPRMSRRELLEAYRVAYTGALERKWLANFHAPAGPMWDKLI
                     PAQATSSTTYAHALASWGVRTLALETAAVMPNTALRVAFTRGAARQHNGSFLYYHAPN
                     FGDTATTFTTLQNFAGPGHYHHSRYGATMGPSLSWYRKNYYLFYMYGAAAIYLEQGFD
                     QFFKPGPGEHPFQLNPLGRITDEFMRFADKHPERGTPYTPVAFLLDPAHGFEMTDYPH
                     LPFGVSPVNRSDRALRELLGTAYHPALVTEGEPATGDRQSLVNGVFGDIFDVLVASEE
                     QADAIDAYRALVVGGHPEWRPRWGVRLEKYVTDGGTVVLNAAQVKGLPADLTGVRALN
                     ATAEADDAACTAQGETQTSLAGQVFRFERVEPRPGTEVLIKAPSGDPVVTVKRVGKGR
                     VVYCAIPDLLGLDERMTPAAAHMLAHLFDAATPVRVRGDIQHLVNRTTRGWAVTLVNN
                     RGVYKPQQGLAQVNRTEEAAVTLELRGAAIQSASEWTTDAALKVEQDAGRDRLRLTIP
                     AGGLRIVELVPGK"
     gene            3050..3322
                     /locus_tag="AC159P_08040"
     CDS             3050..3322
                     /locus_tag="AC159P_08040"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HIR3695442.1"
                     /translation="MKAARHFIISGEVQGVGFRFFAQRVAARHQVTGYVRNLEDGRVE
                     TLAEGTPEGVEGFKHDISAGPAHARVEQVEEVSVEPTGYPQFRIER"
     gene            3448..3969
                     /locus_tag="AC159P_08045"
     CDS             3448..3969
                     /locus_tag="AC159P_08045"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HIR3695443.1"
                     /translation="MDHLKSLIREVPDFPKPGINFYDITTLLKHPEGLRGTVDALCEQ
                     FKGERVDAVVGIEARGFIFAPAMAYHLGAGFVPARKPGKLPSECASVSYDLEYGQDAL
                     QIHSDAVGAGHRVVIADDLLATGGTAKAVVNLVEQLGGEVVGLAFVVELEFLPGREKL
                     AGYDVRSLIKYQS"
     gene            4018..4090
                     /locus_tag="AC159P_08050"
     tRNA            4018..4090
                     /locus_tag="AC159P_08050"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4051..4053,aa:Arg,seq:cct)
BASE COUNT          707 a         1415 c         1307 g          661 t
ORIGIN      
        1 ctctcagtcc ccgctttttt tatccgcggt cgtgccggaa cttgatgaca tacattccca
       61 tcagcgtctc tctcgtcttc cttcttctcg tcagcccctt cgtcacgtca tgcacctctc
      121 acgcgcaggc gacgcagacc cggcccgtcg gcgccgcgca gtcgccgcgc cccgagtatc
      181 tctggtacga ggcggagaac atgagcggcc tctcgacgga cgagcggcac gagccgcggc
      241 agaatccttc gtggcgcagc ccggcgcgcg cggagtcgcc cggctggggc atcaacgggc
      301 cgggcgtctc ggcggagtgg tcgcagggcg gcgagagcga gtggaactcc gtcgcggcga
      361 gtgcggacga gacaaaggct tcaatcgcgc aagacctcga agcgccgcgc gaggggcggt
      421 atcgcctgtg ggtgcgctac gcggactggg tgcggcgggg cgagcagttc acggcgcgcg
      481 tcgtgcagga cgggagggaa gtcttccgcc acgagttcgg agcagcagac ctcgtcgatc
      541 cgaacgacga ggtgagcatg tactggggct gggcgttcgc gtggggcggc tcgaccgagt
      601 tctctctgaa gaagggcgcg gcccgcgtct ctttcgaagt ggagcgcgcg accgaagcgc
      661 ggcgccatgt cgattgcttt gttctgacca acgatgtgaa ctacacgccg gacggacgac
      721 gcaagccgcc cttcgccgcg gcgctcgcgc tcgaaaggtg gggcgcgacg agggcaacgc
      781 tcgcgccgtt gatgagcgcg ccgacggcgg gagacgtggt gcccgagcgt tggaaactgc
      841 ggcgcctcgg gggccgcgac ttccagatgc cgtggaacat cgcggaggag ttctggaaga
      901 tgtacgacca gcccgccgag gcgcggcccg tctacccttt caacgccgag ccgatcgaat
      961 ccttcgtcgg gaagtacaag ggcgtgcgcg aggtgccgat cttctcctcg aagctcgtcg
     1021 tgccggtcgt ctacatcaac cacgtgcagg aacaccttaa agagggcgcg cccttcctcc
     1081 gccacctgcg cgagacgcgc acgcccttcg ccatcctcat caactacggc gcggcgcggt
     1141 tcgccgaagc cgcggcgggg caggaggctt tgaagctgtt gacgggcgag ctgcgcgagc
     1201 agttcatcgg ctggatttcg ggcgagagca tcggccacgt cttcacggat gtccaacaga
     1261 acctcaagct cgacccgcgg atgtcgcggc gcgagctgct cgaagcctac cgcgtcgcct
     1321 acacgggcgc gctcgaacgc aagtggctcg ccaacttcca cgcgcccgcc gggccgatgt
     1381 gggacaagct gatccctgcg caggcgactt cgagcacgac ctacgcgcac gcgctcgcct
     1441 cctggggcgt gcgcacgctc gcgctcgaaa ccgccgccgt gatgccgaac acggcgctgc
     1501 gcgtcgcctt cacgcgcggc gccgcgcgcc agcacaacgg ctcgttcctc tactaccacg
     1561 cgcccaactt cggcgacacg gcgaccacct tcaccaccct gcaaaacttc gccgggccgg
     1621 gccactacca ccactcgcgc tacggcgcga cgatggggcc gtcgctctcc tggtacagaa
     1681 aaaattacta cctcttctac atgtacggtg ccgcggccat ctacctcgaa cagggcttcg
     1741 accagttctt caagccgggg ccgggcgagc accccttcca actgaacccg ctcgggcgca
     1801 tcacggacga gttcatgcgc ttcgccgaca aacacccgga gcgcggcacg ccctacacgc
     1861 ccgtcgcctt cctgctcgac cccgcgcacg gcttcgagat gaccgactac ccgcaccttc
     1921 cgttcggcgt ctcgcccgtt aacaggagcg accgcgcgct gcgcgaactc ttgggcaccg
     1981 cctatcatcc cgcgctcgtt accgaaggcg aacccgcgac cggcgaccgc cagtcgctcg
     2041 tcaacggcgt cttcggcgac atcttcgacg tgctcgtcgc ctcggaagaa caggccgacg
     2101 ccatcgacgc ttaccgcgcg ctcgtcgtcg gcggccaccc cgaatggagg ccgcgctggg
     2161 gcgtgcgtct tgaaaagtat gtgaccgacg gcggcaccgt cgtcctcaac gccgcgcagg
     2221 tcaaaggact ccccgcggac ttgaccggcg tccgcgcgct gaacgcgact gccgaggcgg
     2281 acgacgccgc ttgcaccgcg cagggcgaga cgcagacgag cctcgccggt caggtcttcc
     2341 gcttcgagcg cgtcgaaccg cgtcccggca cggaggttct catcaaggct ccatcgggcg
     2401 accccgtcgt caccgtcaaa cgcgtcggca aggggcgcgt cgtctactgc gccatccccg
     2461 acctgctcgg actcgacgag cgcatgacgc ccgccgccgc gcacatgctc gcgcacctct
     2521 tcgacgccgc gacgcccgtc cgcgtccgcg gcgacataca gcacctcgtc aaccgcacca
     2581 cgcgcggctg ggccgtcacc ctcgtcaaca accgcggcgt ctacaagccg caacagggac
     2641 tcgcgcaggt caaccgcacc gaagaggccg ccgtcacgct cgaactgcgc ggcgccgcca
     2701 tccaaagcgc ctccgagtgg acgaccgacg ccgccttgaa agtcgagcag gacgcgggcc
     2761 gcgaccgcct gcgcctcacc atccctgcgg gcggcctgcg catcgtcgaa ctcgtgccgg
     2821 gaaaatgaaa ggctgttagc tgttagccgt tagctgttag cttttggctt aacggctgac
     2881 ggttgatggg agcggcgctg tcatcagtcg tcaagcatca agctaacagc taacggctaa
     2941 cagctaacag ctaacagccc taccgcgttt gatttttcgt ccggccatct tttacgatag
     3001 cgcgtctttc aagaccgatt cgcgcgcggt tcgagggggc gttcttgcga tgaaggcggc
     3061 acggcatttc atcatcagcg gcgaggtgca gggcgtgggg ttccgcttct tcgcgcagcg
     3121 cgtcgcggca cggcatcagg tgacgggcta cgtgcgcaac ctcgaagacg ggcgcgtcga
     3181 gacgctggcc gagggcacgc ccgagggcgt cgagggcttc aagcacgata tcagcgccgg
     3241 ccccgcacac gcccgcgtcg aacaagtcga agaggtcagc gtcgagccga caggctatcc
     3301 ccaattccga atcgaaaggt gaaaaagcag taggcagcgg gcagcagaca gcaggcagtt
     3361 aaggaatact gaatttcagt tattgaaatt aacaattctt cgattgtctt cactgcctgc
     3421 tgcctgctgc ccgctgcccg ctctctcatg gatcatctca aatctctcat ccgcgaggtg
     3481 cccgacttcc cgaagccggg cattaatttc tacgacatca cgacgctgct gaagcacccc
     3541 gagggtttgc gcgggacggt ggacgcgctg tgcgagcagt tcaagggcga gcgggtggac
     3601 gcggtcgtcg gcatcgaggc gcgcggcttc atcttcgcgc cggcgatggc ctaccacctc
     3661 ggcgcgggct tcgtcccggc gcgcaagccg ggcaagctgc cgtcggagtg cgcctccgtc
     3721 tcctacgacc tcgaatacgg gcaggacgcc ttgcagatac acagcgacgc cgtcggcgcg
     3781 ggccaccgcg tcgtcatcgc cgacgacctg ctggccaccg gcggcacggc gaaggccgtc
     3841 gtcaacctcg tcgaacagct cggcggcgag gtggtcggcc tcgccttcgt ggtggagctg
     3901 gagttcctgc ccggcaggga aaagctcgcg ggctacgacg tgcgctcgct catcaagtac
     3961 cagtcctgac ggcaactgga cgccgccgcg gcgcgtctgt taagttgcaa gactccggct
     4021 cccgtagctc agtggataga gcactcgcct cctaagcgag gggtcgcgcg ttcgattcgc
     4081 gccgggggca
//
LOCUS       DBNGOH010000409         1920 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_430229, whole genome shotgun sequence.
ACCESSION   DBNGOH010000409 DBNGOH010000000
VERSION     DBNGOH010000409.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1920)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1920)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1920
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_430229"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..612)
                     /locus_tag="AC159P_08055"
     CDS             complement(<1..612)
                     /locus_tag="AC159P_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015114347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbnB/TglH/ChrH family RiPP precursor
                     modification enzyme"
                     /protein_id="HIR3695444.1"
                     /translation="MSEVFSRLPSLGVGVGFREQFRADVFLHRGDVDFLEITADHYLD
                     APPEKLRELELLAEHFTLIPHALNLSLGSAEGLDTVYLDKLARLVNRLDPPWWSEHVS
                     FTRAGGVDIGHLSPTPFNEQSVAVLARNVAEARARIGAPLILENITYMLEVPGASMGE
                     AEFLAEALERTGCGLLLDITNLHINAFNHRYDWREFLRRLPLER"
     gene            complement(609..1673)
                     /locus_tag="AC159P_08060"
     CDS             complement(609..1673)
                     /locus_tag="AC159P_08060"
                     /inference="COORDINATES: protein motif:HMM:TIGR04222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="HIR3695445.1"
                     /translation="MDWLMNNPLADMYGPLFLFVYAGVIAATAIVCRVMTRQLDWTAK
                     MQPPPVPSDPDPHEIAYLRGGENEVTRSLIFALVQRGLLRTTEAGMVERVWEKKETGH
                     LSPIERRAYDYFLVPYKTSDIYKTGALASQLKPFCDVYEQRLQRERLLTTDDVRQTAR
                     MVVLAGASVVLGLGAYKLIVAVAEGRYNVIFLMAFGLIGVFVIAAACRLPRVSRRGRA
                     YLERLQLAFQRLKWQAQAAPALAAPNGGAAATVHGLDPSMLLLVGVFGVGALAGTQYD
                     SFEQSFHRAAVAKNNADASGGGSGCGSSCGSSDSSSGSWFGGGGDSGSSSSDSGSSCS
                     SGSSCGSSCGGGGCGGGCGG"
BASE COUNT          329 a          651 c          632 g          308 t
ORIGIN      
        1 ccgctccaaa ggaagccgcc gcaggaactc gcgccaatcg tagcggtggt tgaaagcgtt
       61 gatgtgcagg ttcgtgatgt cgagcagcag accgcagccc gtgcgttcga gcgcctcggc
      121 gaggaactcc gcctcgccca tcgaggcgcc cggcacctcc agcatgtacg tgatgttttc
      181 gaggatgagc ggcgcgccga tccgcgcgcg cgcctcggcg acgttgcgtg cgaggacggc
      241 gacggactgt tcgttgaaag gcgtcggcga aaggtggccg atgtccacgc cgcccgcgcg
      301 cgtgaacgag acgtgctcgc tccaccacgg cgggtcgagc cggttgacga gccgcgcgag
      361 cttgtcaaga tagactgtgt ctaaaccttc ggcgctgccg agcgagaggt tgagcgcgtg
      421 ggggatgagc gtgaagtgtt cggccagcag ctccagctcg cgcagctttt cgggcggcgc
      481 gtcgaggtag tggtcggcgg tgatttcgag gaagtccacg tcgccgcggt gcaggaacac
      541 gtcggcgcgg aactgctcgc ggaagccgac cccgacgccc agcgacggca ggcgcgagaa
      601 cacttcgctc accctccgca accgccgccg cagccgccac cgccgcaact gctcccgcaa
      661 ctactgccgc tgctgcaact gctcccgctg tcgctgctgc tgctgccgct atcgccgccg
      721 ccgccgaacc aactgcccga tgagctgtcg gacgacccgc atgacgagcc gcaaccgctg
      781 ccgccgcccg acgcgtccgc gttgtttttg gcgaccgccg cgcggtggaa cgactgctcg
      841 aacgagtcgt actgcgtccc cgcgagcgcg ccgacgccga acaccccgac gagcaggagc
      901 atcgaagggt cgaggccgtg caccgtcgcc gccgcgccgc cgttcggcgc ggcgagcgcg
      961 ggcgcggcct gcgcctgcca cttcaaacgc tggaacgcga gctgcagccg ttcgaggtac
     1021 gcgcgcccgc ggcggctcac gcgcggcagc ctgcacgccg ccgcgatgac gaacacgccg
     1081 atcaggccga aggccatgag gaagatgacg ttgtagcgcc cctcggcgac cgccacgatg
     1141 agcttgtacg cgccgagtcc gagcaccacg ctcgcccccg ccagcacaac catccgcgcc
     1201 gtctgccgca cgtcgtccgt cgtgagcagc cgctctcgct ggagccgctg ttcgtaaacg
     1261 tcgcagaacg gcttgagctg cgaagccagc gcgcccgtct tgtagatgtc ggaagttttg
     1321 taagggacaa ggaagtagtc gtatgcgcgc cgctcgatgg gcgagaggtg gccggtctct
     1381 ttcttctccc acacgcgctc gaccatgccg gcctcggtcg tgcgcagcag tccgcgctgg
     1441 acgagcgcga agatgagcga gcgcgtcact tcgttctcgc ccccgcgcag gtacgcgatc
     1501 tcgtgcgggt ccgggtcgga agggacgggc ggcggctgca tcttcgccgt ccagtcgagc
     1561 tgccgcgtca tcacgcggca cacgatggcc gtcgccgcga tgaccccggc gtagacgaag
     1621 aggaagagcg gcccgtacat gtctgccagc gggttgttca tcaaccagtc catgatgctt
     1681 cacctctcaa acagctttac actgatacgc gccccccgcg cccgttgttt gatgatgtaa
     1741 cagaaaatta atctcgtgat gaagcatgga ttttaccacg tggcgacagt tcgaatccgc
     1801 cgcgtagcgg cagttgagtt tagcccggcg tttcaaggcc gggtgaggga cacataaccc
     1861 ggcccgcctc gcgttagcga cgcctgaaca tggattcaat cgtcgctacg cgacaggatg
//
LOCUS       DBNGOH010000410         2180 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1164374, whole genome shotgun sequence.
ACCESSION   DBNGOH010000410 DBNGOH010000000
VERSION     DBNGOH010000410.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2180)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2180)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2180
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1164374"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2180)
                     /locus_tag="AC159P_08065"
     CDS             complement(<1..>2180)
                     /locus_tag="AC159P_08065"
                     /inference="COORDINATES: protein motif:HMM:NF016768.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate synthase central domain-containing
                     protein"
                     /protein_id="HIR3695446.1"
                     /translation="ATEPEFWQVAALPRTARGLADKDIFHLTLRLERELPVHIASLSP
                     NSVIYKVRGDAETLKRYFPELSHPDFKSAITLGHGRYSTNTDSRAERAQMFSTLGHNG
                     EINSIDRLGREAVSLGFQLPERASDSQVLDRVVESFMFDYDLSLMNALEIAFPPVWSE
                     IARFPAELRELYRYWRRAFGALAQGPAAIIARQADEIVFSSDALGLRPLWCGETEKEF
                     FASSEKGVVPLEQMVRDPRPLAPGEKMGCLLERGEGVRVFEYHELQRRALEDVRKRIP
                     LARLNDLTHWFDETKSEVGSQNAEPKTTGSEFILQHSDFCLDERRLIALGWNRLDAVD
                     IVEMAAAGKEPISGLGWDGPLAALSKQRKNVADFFHERVAVVTNPAIDREREADHFST
                     RVFLGARPCLARPAESGRQIELRTPLLLGGARGDEAEHARVAREMRQRTLAEVLALCG
                     EVLGAEALALGAAAGAGSEEGAAAKVDEGAGAPHGALRAATIDTSYDEGETLEQALKR
                     LEEEAARLVRESKMSVLVLDDARAHQGARLGIDAHLALAAVDARLRSERDAEGVSLRR
                     HASLVLRAGALRNVHDLVFALGCGADAVNPYTLFEVAARGAKDDEERRKRLRNVVNAL
                     EVGVEKVLSTMGIHELDGYGRLFAAVGLAPDVADYFGTANYCGSDSAGLSLARMDADA
                     RERLKVAAGVVEDEAPKRRPPILQGEFREQPSIWNVVGKVARGE"
BASE COUNT          311 a          736 c          782 g          351 t
ORIGIN      
        1 ctcgccgcgc gcgacctttc cgacgacgtt ccagatcgag ggctgctcgc ggaactcgcc
       61 ctgcaagatc ggcggccttc gcttcggcgc ttcgtcttcg acgactccgg cggcgacctt
      121 caggcgctcg cgcgcgtcgg cgtccatgcg ggcgagcgag aggcctgcgg agtccgagcc
      181 gcagtagttg gccgtgccga agtagtcggc cacgtcgggc gcgaggccga ccgccgcgaa
      241 cagacgaccg tagccgtcga gttcgtgaat gcccatcgtc gagagcactt tctcgacgcc
      301 gacttcgagc gcattgacca cgttgcgcaa acgcttgcga cgctcctcgt cgtctttcgc
      361 gccgcgcgcg gcgacctcga agagcgtgta agggttgacg gcgtcggcgc cgcagccgag
      421 cgcgaagacg aggtcgtgga cgttgcgcag ggcaccggcc cgcagcacga ggctggcgtg
      481 acggcggagg ctgacgcctt ccgcgtcgcg ctccgagcgc agccgcgcgt ccacggcggc
      541 gagcgcgagg tgcgcgtcga tcccaagacg cgcgccttga tgagcgcgcg cgtcgtcgag
      601 gacgaggacg gacatcttgg actcccgcac gagccgcgcg gcctcttctt cgaggcgctt
      661 caaagcctgt tcgagtgtct cgccctcgtc gtaactcgtg tcaatcgtcg ccgcccgcag
      721 cgcaccgtgc ggggcaccgg cgccttcatc aaccttcgcg gcggcccctt cttcgctgcc
      781 tgcgcccgcc gcggcgccga gcgcgagcgc ttccgctccc aagacctcgc cgcagagcgc
      841 gagcacttcg gcgagcgtgc gttgacgcat ctcgcgcgcg acccgcgcgt gctccgcctc
      901 gtcgccgcgg gcgccgccca agaggagcgg cgtgcgcagc tcgatctgcc gcccgctctc
      961 ggcgggccgg gcgaggcagg ggcgcgcgcc gaggaagacg cgcgtcgaga agtggtcggc
     1021 ctcgcgctcg cggtcgatgg cggggttcgt caccaccgcg acgcgctcgt ggaagaagtc
     1081 ggcgacgttc ttgcgctgct tcgagagcgc ggcgagcggg ccgtcccagc cgagtccgct
     1141 gatcggctcc ttgcccgccg ccgccatctc cacgatgtca acggcgtcga gccggttcca
     1201 gccgagcgcg atgagacgcc tttcatcaag gcagaagtcg gaatgctgaa ggatgaactc
     1261 ggagccggtg gtcttcggtt cagcgttctg acttccgact tctgactttg tctcgtcgaa
     1321 ccagtgcgtg aggtcgttga ggcgcgcgag cgggatgcgc ttgcgcacgt cttcgagcgc
     1381 gcggcgttgc agctcgtggt actcgaagac gcggacgccc tcgccgcgtt cgagcagaca
     1441 gcccatcttc tcgccgggcg cgagcgggcg cgggtcgcgc accatctgtt cgagcgggac
     1501 gacgcccttc tcggacgagg cgaaaaattc cttctccgtc tcgccgcacc agaggggccg
     1561 caggcccagc gcgtcggagc tgaagacgat ctcgtcggcc tgccgcgcga tgatcgccgc
     1621 ggggccttgc gcgagcgcgc cgaaggcgcg gcgccagtag cggtacagct cgcgcagctc
     1681 cgcggggaag cgcgcgatct cgctccacac gggcgggaag gcgatctcca aagcgttcat
     1741 caggctcagg tcgtagtcga acatgaagct ctcgacgacg cggtcgagca cctgcgaatc
     1801 gctggcgcgc tcgggcagtt ggaagccgag cgagacggcc tcgcgcccga ggcggtcgat
     1861 ggagttgatc tcgccgttgt ggccgagcgt ggagaacatc tgcgcgcgtt cggcgcgcga
     1921 gtcggtgttg gtcgagtagc gaccgtggcc gagcgtgatg gccgacttga agtccgggtg
     1981 cgacagctcc gggaagtagc gcttgagcgt ctcggcgtcg ccgcgcactt tgtagatgac
     2041 gctgttcggg ctgagcgagg cgatgtggac gggcagttcc ctttcgagcc gcagcgtgag
     2101 gtggaagatg tctttgtcgg cgaggccgcg cgccgtccgc ggcagcgccg cgacctgcca
     2161 gaactcgggc tcggtggcgc
//
LOCUS       DBNGOH010000411         2253 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2262448, whole genome shotgun sequence.
ACCESSION   DBNGOH010000411 DBNGOH010000000
VERSION     DBNGOH010000411.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2253)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2253)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2253
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2262448"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            529..>2253
                     /locus_tag="AC159P_08070"
     CDS             529..>2253
                     /locus_tag="AC159P_08070"
                     /inference="COORDINATES: protein motif:HMM:NF021778.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HIR3695447.1"
                     /translation="MLRSSADFSAHVVVGSGAPPSLPTRARALLVVCAWLLVVCASSP
                     VQAQPSQPPGGASSRASQRHTHEGVTIEFTVEPVAGGKAAELVEGAEAVVKFSITDGA
                     GRAVGNLRPAAWMHARRTDKAPDAAACREKVRSFLQAGLTNRPDIDLNTFFVLALNHE
                     PNISVIDPLSGFGTTKLYTLVALPAPGEDWVLSGDRKRLYVSVPAAGEVVAVDTATWK
                     TLAHIDAGTRPTRLALQRDEKYLWVGNDGPAETGGVTAIDTGTLKVAAHIKTGAGRHE
                     IAFTDDDRRAFVTNKESGTLTVIDVPSLARVTEHKVGAQPSALAFSSLGKAVYVVAEA
                     DGAVTVVAGSGEAVVARLKSRPGLHAIRFTPDGRFGFVVNRAASEVYVFDVSNNRLVQ
                     SVAVEPSPDQITFTRDFAYVRATGSEFVSMIKLRDLGTENAEAAAGRFPAGQRAPRES
                     PSVSIAASVVPAPEGGSVLAANPADKTIYYYSEGMAAPMGSFTNYRRDPRAVLVLDKS
                     LRETAPGVYTTTARFNGHGQYDVAFLLDSPRVVGCFAAAVKENPELPKQRGVPIKVEM
                     LAGGGAARV"
BASE COUNT          363 a          783 c          772 g          335 t
ORIGIN      
        1 aacgcaaccc cgtgacgagt tgaattgcgc ccatcaggga agtcgtagag gtgacacggc
       61 tcagtgagtc tggggcagtg aagccaagcg ggagcgtgtg agccgacgcc gaagacttgt
      121 caagaacacc cccgcgggct agcgaagcgg tgcgaggaga tatgtcttgc cgaaacgggc
      181 ggaactatat gcctgctctc ctcggcgcgt caagaatctt ccgccgcgcg cgtctgctgc
      241 cgtggaagaa caggcgttcg ggcgggtcgg aaaaactctt gacagttggc ggacgcgcgc
      301 catagattct tgggcgactc gttcgataag tttgaaggca cccggactcg cgcggcctca
      361 cgttgcaccc tgaatcgaat ttccgcggtc acacgtcggc ttgttcgtct ttactcatcg
      421 ccggaagtct ccccggacac cgcccgcgcg cgagtcgatg agaggtcgcg tgacgcgacc
      481 cgccggggcg acggaagcat catgaagaac aactttttcg cacgcgctat gttgagaagc
      541 agcgcggatt ttagcgccca cgtagttgtc ggaagcggcg cgccgccgtc tcttccgacg
      601 cgcgctcgcg cgctgctcgt cgtctgcgcg tggctgctcg tcgtgtgcgc gtcgtcgccg
      661 gttcaggcgc agccgtcgca gccgccgggc ggcgcctctt cgcgcgcgtc gcagcggcac
      721 acgcacgagg gcgtgaccat cgagttcacg gtcgagccgg ttgcgggggg caaggccgcc
      781 gagctggtgg agggcgcgga ggcggtcgtc aagttcagca tcacggacgg cgcgggccgt
      841 gccgtcggca acctgcggcc cgcggcgtgg atgcacgcgc gccggacgga caaggcgccg
      901 gacgccgccg cgtgccgcga gaaagtccgc tcgttcctgc aagcgggcct gaccaaccgc
      961 cccgacatag acctcaacac cttcttcgtc ctcgcgctca accacgagcc gaacatctcg
     1021 gtcatcgacc cgctctccgg cttcggcacg accaagctct acacgctggt cgcgctgccc
     1081 gcgcccggcg aggactgggt cttgagcggc gaccgcaagc ggctctacgt ctcggtgccg
     1141 gcggcgggcg aggtcgtcgc cgtggatacc gcgacgtgga agacgctcgc gcacatcgac
     1201 gccgggacga ggccgacgcg cctcgctttg cagcgcgacg agaagtacct gtgggtcggg
     1261 aacgacggcc ccgccgagac gggcggcgtc acggccatcg acaccggcac gctcaaggtc
     1321 gccgcccaca tcaagaccgg cgcgggccgc cacgagatcg ccttcacgga cgacgaccgc
     1381 cgcgccttcg tcacgaacaa agagagcggg acgctgaccg tcatcgacgt gccgagcctc
     1441 gcgcgggtca cggagcacaa ggtcggcgcg cagccttcgg cgctggcctt ctcttcgctc
     1501 ggcaaggcgg tctacgtcgt cgcggaggcc gacggcgcag tgacggtcgt cgccgggtcg
     1561 ggcgaggcgg tcgtggcgcg cctgaagtcg cggcccggcc tgcacgccat ccgcttcacg
     1621 cccgacgggc gcttcggctt cgtcgtcaac cgcgccgcga gcgaggtcta cgtcttcgac
     1681 gtgtcgaaca accggctggt gcagtcggtc gccgtcgagc cgtcgcccga ccagatcacc
     1741 ttcaccagag acttcgccta cgtgcgcgcc acgggcagcg agttcgtctc gatgatcaag
     1801 ctcagagacc tcggcacgga gaacgcggaa gccgccgcgg gccgcttccc cgcggggcag
     1861 cgcgcgccgc gcgaatcgcc ttcggtctcg atcgccgcct cggtcgtccc tgcgcccgaa
     1921 ggcggctcgg tgctcgcggc gaacccggcg gacaagacca tctactacta cagcgaaggc
     1981 atggcggcgc cgatgggcag cttcactaac taccgccgcg acccgcgcgc cgtgctcgtc
     2041 ctcgacaaga gcctgcgcga gaccgcgccc ggcgtctaca ccaccaccgc gcggttcaac
     2101 ggccacggcc agtacgacgt ggcgttcctg ctcgactcgc cgcgcgtggt cggctgcttc
     2161 gccgccgccg tcaaggagaa ccccgagctg ccgaagcaga ggggcgtgcc gatcaaggtc
     2221 gagatgctgg cgggcggcgg cgcggcgcgc gtc
//
LOCUS       DBNGOH010000412         8209 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3546071, whole genome shotgun sequence.
ACCESSION   DBNGOH010000412 DBNGOH010000000
VERSION     DBNGOH010000412.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8209)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8209)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8209
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3546071"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..129)
                     /locus_tag="AC159P_08075"
     CDS             complement(<1..129)
                     /locus_tag="AC159P_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014394656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HIR3695448.1"
                     /translation="MSDAAVVAPLRVLVVDDEPLAREKIRRMAKDDPEVRIVGECTN"
     gene            complement(397..1563)
                     /locus_tag="AC159P_08080"
     CDS             complement(397..1563)
                     /locus_tag="AC159P_08080"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3695449.1"
                     /translation="MNTTTDTDIRPADAAGLGTGTHWWQRRWGQWAIVFGVWTVIAFI
                     FTGMLYYSQLLGERPMTWGRAAAYQFIYPYLWAAGTMIVLWLARRFPLEGRHLRRRIF
                     YHFLFATLSAILVTGSYQIIYHFLFLNGPDKPYSPWTTLSWIIFNSSENYGIYGLLLL
                     FTHAASYYRRYREGELRAVQLQAQLSQAQLQALKMQLHPHFLFNTLHSISALVHKDPE
                     AADRMIARLGDFLRLTLENSGTQEVTLQKELEFLQCYLEIERIRFQDRLTTRMEIDSR
                     SLDSRVPNLILQPIVENALRHGIAPRSTPGRIEISAKRNNGSVRIEVKDNGPGISAIS
                     ATNGRFREGLGLTNTRARLERLYGEAHRFELANDPHGGLTVTLEIPAHGKEAAA"
     gene            2048..3088
                     /locus_tag="AC159P_08085"
     CDS             2048..3088
                     /locus_tag="AC159P_08085"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val family dehydrogenase"
                     /protein_id="HIR3695450.1"
                     /translation="MEITEKRVEGYERVLFGEEPESGYRGIIAVHSTALGPAIGGTRF
                     WNYASEGEALVDALRLARGMTYKNALAGLPFGGGKSIIAGDSRTAARAPLFRAHGRFV
                     ERLGGRYVTAEDVGTSPADMEHIRAETEHVAGLIGKSGDPSPVTARGVFRAIQAAAKF
                     RWGADELGGRRVALQGCGNVGRNLAHHLARAGARLVATDADEEKLRRVVAECGAAAVA
                     PGEIYDVEADIFAPCALGGVLNDETIPRLKVEVVAGAANNQLLDARHGEALERRGITY
                     APDYVANSGGVIHGCNIELLGGRAEDALRRVDEIYDTLLRIFDAAKREGVPAHVAADR
                     LAEQRLRAARSD"
     gene            3232..3789
                     /locus_tag="AC159P_08090"
     CDS             3232..3789
                     /locus_tag="AC159P_08090"
                     /inference="COORDINATES: protein motif:HMM:TIGR02777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase ligase N-terminal
                     domain-containing protein"
                     /protein_id="HIR3695451.1"
                     /translation="MRGKAKFVVQEHHASRLHWDFRLEIGGTLKSWAVPKGPSLDSSV
                     KRLAVEVPDHALSYLTFTGVLPEGTYGAGPVYRWDIGTFEPSSDEEPARSWREGSLRF
                     RLDGERLKGEWLLFRMKGRSERGRPPWLLQKLRDEFAVAGHEAEVVGEVKSRKAVAKP
                     ATKKKAGAKKRATKKRAKAGGRKSS"
     gene            3786..4523
                     /locus_tag="AC159P_08095"
     CDS             3786..4523
                     /locus_tag="AC159P_08095"
                     /inference="COORDINATES: protein motif:HMM:NF037435.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF434 domain-containing protein"
                     /protein_id="HIR3695452.1"
                     /translation="MSPDRRQHRGAHPEDARLFEDARLPSLRRATGELSWLLSRGYRL
                     ASSLKLVGDRHALRERQRLAVSRAACSDDSLEGRSARRVGPESVRGAELVVDGFNVVI
                     TAEAALGGGVLVRCRDGCVRDMSSVHGSYRSVDETERALLVVGEALERLGPRSVEWLL
                     DRPVSNSGRLTARIEQLAAARGWPWTVALEFNPDRSIVASPRIAVTSDSNILDGARAW
                     LNFNDYLVERDLPQAWLIDLQASIGPS"
     gene            complement(4489..5685)
                     /locus_tag="AC159P_08100"
     CDS             complement(4489..5685)
                     /locus_tag="AC159P_08100"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HIR3695453.1"
                     /translation="MEESRTVELFAGIGGFRIAADRCGFKTVWANDNSPKACTVYRDA
                     FGDSVLREGNVNGLTNTIPPHDLLTAGFPCQPFSSAGKKEGIRDSRGTLFQTIATILA
                     RHQPTYFVLENVKRLLLMEQGSHFATILSVLADLDYWIEWRLLNAMHFGLPQNRQRVF
                     LIGVHRNKYPSHGSPPIRLATKEDLSELSGNRLDELLHPADWKGIAEHGQRIPAWGIA
                     SQGRFFAATLSTFSERSPLTPLRSVLETEVSSDFDFTESTLERLKGSKPVMRYIDGVE
                     ILSNQSGGARMGYTVFGIDGVAPTLTATTSRHYERYKVGERYRRLTNTEYARIQGFPD
                     DHCKAVSVHDQYGLIGNAVPPRMADWVLKKLLGDGSYATAPSRPRPGAERCGVVKTAQ
                     CSPVSR"
     gene            complement(5663..6112)
                     /locus_tag="AC159P_08105"
     CDS             complement(5663..6112)
                     /locus_tag="AC159P_08105"
                     /inference="COORDINATES: protein motif:HMM:TIGR00632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="very short patch repair endonuclease"
                     /protein_id="HIR3695454.1"
                     /translation="MGDFLSRPKRSEHMSKIRAKNTGIERIVFRLLKRKRVYFATHAS
                     ALPGRPDIVFRRCKLAVFIDGDFWHGRNLKAWEGKLDDFWKAKIKRNIERDREKDRKL
                     RALGWRVLHLWGTDIKRDPEKCVDKILAVRERLLRPSESGNGRVENG"
     gene            6318..6491
                     /locus_tag="AC159P_08110"
     CDS             6318..6491
                     /locus_tag="AC159P_08110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695455.1"
                     /translation="MAKSKKARRLDVLSAQVRVCTRCPLHESRTHAVPGDGKYAAKVK
                     EDCALLKRELKKL"
     gene            6495..8153
                     /gene="ligD"
                     /locus_tag="AC159P_08115"
     CDS             6495..8153
                     /gene="ligD"
                     /locus_tag="AC159P_08115"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF045432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HIR3695456.1"
                     /translation="MGLETYRKMRDFRRTPEPKGEVSRANRRRFVVQEHHASKLHFDF
                     RLEMGGVLKSWSIPRGPSLDPAERRLAVPTEDHPVEYLKFEGQIPEGEYGAGRHLKWD
                     AGTYELVEGDDPLAQLAAGKLTFELKGRKLRGSFSLVRTHLRGADESWLLFKREDEYA
                     EPGWVLELRQPIDGQRTLANRKRAKADERPRGEAHKEVRRFKTGAGGGKVVPVSSALK
                     KKEVGGNVNVKVGREVVPLTHLDRVYWPDEGFTKADLVRYYDEVSAYILPYLEDRPLI
                     MKRYPAGIGGPSFHQHDVNEAPEFVRTVRHRATEDGHEVDYIVGGDRATNLYMANLGA
                     IERHPWHSRVGHLEHPDWFVFDLDPGAGVPAETICEVAVAVRDVLERIGLKSYAKTSG
                     SRGIHVYVPVKSVYGYEEVAGLAEQVATIVAREKADVATVERALAKRKRGQIYVDHMQ
                     NARGKSVVAPYSVRPKPGAPVSAPLEWQEVERKKITPRQFDITNMGRRLASEGDLFRA
                     VLTKRQSLGAAFEAAKKLYEEERAGRARVRPAAANGRRNGSQSS"
BASE COUNT         1475 a         2538 c         2809 g         1387 t
ORIGIN      
        1 gttcgtgcac tcgccgacga tgcgcacctc cgggtcgtcc ttcgccatgc ggcgaatctt
       61 ctctcgtgcg agcggctcgt cgtccacgac cagcacgcgg agcggcgcta ctacggcggc
      121 gtcagacata acagacgtga accgtcaacc ctgatgaatg taaagccgcg aaccgtacac
      181 cgcgacgggc gaaagaggtg ttccgcaaaa gagtaggtga cgacaagttg gaagaagttt
      241 cactaacttg ccgccgcccg cgtcttttcg cggctgacgg attacggctc taaagtaaaa
      301 caccgcgccg ggcgattccg tggcaggaat ctccccgcgc tgcgaaagtt ggaaagtaaa
      361 gcgtcgattg gacagcggac ggcggcgagc gcgtcttcag gcggcggcct ctttgccgtg
      421 ggccggtatt tcgagcgtca cggttagacc gccatgggga tcgttggcta actcgaagcg
      481 gtgcgcctcg ccgtaaagcc gttcgaggcg cgcgcgcgtg ttcgtcaacc cgagaccctc
      541 ccgaaacctg ccgttcgtcg ctgagatggc tgaaatgccc ggcccgttgt ccttcacctc
      601 tatacggacg gagccgttat tccgtttcgc ggaaatctcg atgcgtcccg gtgtcgaacg
      661 tggtgcgatt ccgtgtcgga gggcgttctc gacgatgggc tggaggatga ggttcggcac
      721 gcgcgagtcg agcgagcgcg agtcgatttc catgcgggtg gtgagccggt cctggaagcg
      781 gatgcgctcg atctccaggt agcactgcaa gaactcaagc tccttctgga gcgtcacctc
      841 ctgcgtgccg gagttttcga gcgtgaggcg gaggaagtcg cctaaacgcg cgatcatgcg
      901 gtcggcggcc tccgggtctt tgtggacgag ggcggagatg gagtggagcg tgttgaaaag
      961 gaagtgcggg tggagctgca tcttcaaagc ctggagctgc gcctgcgaga gctgcgcctg
     1021 aagctgcacg gcgcgcagct cgccctcgcg gtagcggcgg tagtagctgg cggcgtgggt
     1081 gaacagcagc agcagcccgt agatgccgta gttctcgctc gaattgaaga tgatccagct
     1141 cagcgtcgtc cacggcgagt agggcttgtc ggggccgttg aggaagagga agtggtagat
     1201 gatctggtag ctgccggtga cgaggatggc cgagagcgtg gcgaagagga agtggtagaa
     1261 gatgcgccgc cggaggtggc ggccttcgag cgggaaacgg cgcgcgagcc agagcacgat
     1321 catcgtcccc gcagcccaca ggtacgggta gatgaactgg taggccgccg cgcgccccca
     1381 cgtcatcggg cgctcgccga ggagctgcga gtagtagagc atccccgtga agatgaacgc
     1441 gatcaccgtc cagacgccga agacgatggc ccactgcccc cagcggcgct gccaccagtg
     1501 ggtgccggtg ccgaggcccg ccgcgtcggc gggtcgaatg tcggtgtcgg ttgtcgtgtt
     1561 catggcgttc ggcggtgctc gttgaaagac gactcgctgc ttcggcgttc ctggccggga
     1621 acatcgactg ccggggcggg ccggacgggc ggaaggtgac gcggccaaca atctgacggt
     1681 cgctctacgg actgctcccg tgctcgcgcg aaacacttga atatagaccc gcgcgccgcc
     1741 cgttggaaag cgcggtgtga cgaagcggac tcgtgacgtg acgagacggc cctaatctcg
     1801 gattttggat ttccggttcc ggatttggat taatggccgc cggggaaatg aggggaaaag
     1861 gttaaagggg gaagagggga agaagcaaag acaaaaaaac tcctcgcctc tttccctttt
     1921 aaccttttcc cctcttcccc ctttaacctt ttcccctttg cggaagtggc acctaacggc
     1981 gcgcgccgag cggccctgtg cgccccgcgc aaactcgtgt agaagaattt tcgtagagag
     2041 ctgtgctatg gagatcaccg agaagagagt cgaaggctac gagcgcgtcc tgttcggcga
     2101 ggagccggag tcgggctacc gcggcatcat cgccgtccac agcaccgcgc tcggcccggc
     2161 catcggcggc acgcgcttct ggaactacgc gagcgaaggg gaagcgctcg tggacgcgct
     2221 gcggctcgcg cgcgggatga cctacaagaa cgcgctggcg gggctgccct tcggcggcgg
     2281 caagtcgatc atcgccggcg acagccggac ggcggcgcgc gcgccgctct tccgcgcgca
     2341 cggtcgcttc gtcgagcgcc tcggcgggcg ctacgtcacc gccgaagacg tgggcaccag
     2401 ccccgccgac atggagcaca tccgcgcgga gaccgagcac gtcgccggcc tcatcggcaa
     2461 gtcgggcgac ccctcgcccg tcacggcgcg cggcgtcttc cgcgccatcc aggcggcggc
     2521 gaaattccgt tggggcgcgg acgaactcgg cggcaggcgc gtcgcgcttc agggctgcgg
     2581 caacgtcggg cgcaacctcg cgcaccacct cgcccgcgcg ggcgcgcggc tggtcgcgac
     2641 ggacgccgac gaagagaagc tgcgccgcgt cgtcgcggag tgcggcgccg cagccgtggc
     2701 gcccggagag atttacgacg tggaagccga catcttcgcg ccttgcgcgc tcggcggcgt
     2761 gctgaacgac gagacgatcc cgcggctgaa ggtggaggtc gtcgccgggg ccgccaacaa
     2821 ccaactgctc gacgcgcgcc acggcgaggc gctcgaacgg cgcggcatca cctacgcgcc
     2881 cgactacgtc gccaactcgg gcggcgtcat ccacggctgc aacatagaac tgctgggcgg
     2941 tcgcgccgaa gacgcgttgc gtcgggtgga cgagatttac gacacgctgc tccgcatctt
     3001 cgacgcggcg aagcgcgaag gcgtgcccgc gcacgtcgcc gcagaccggc tcgccgaaca
     3061 gcgcctgcgc gcggcgcgga gcgattagcc gcggcgacac ggagataaaa caagagacgg
     3121 aagagacatc gacgaagggg agagtcgaat gcttcgactc tccccttcgt cgatgtctct
     3181 tcttcgtttt catccccgcg ccttcgtgcc tctgtggcaa aatctttccc gatgaggggt
     3241 aaagcgaaat tcgtcgtgca ggagcaccac gcgagccgcc tgcactggga cttccgtctg
     3301 gagatcggtg ggacgctcaa gagttgggcc gtgccgaagg ggccttcgct cgattcgtcg
     3361 gtgaagcggc tcgccgtcga ggtgccggat cacgcgctct cctatctgac cttcacaggc
     3421 gtcctgcccg aaggcacgta cggcgcgggg cccgtctacc gctgggacat cggcacgttc
     3481 gagccttcgt cggacgaaga gcctgcgcgg agttggcgcg aggggagtct gcgtttccgc
     3541 ctcgacggcg agcggctgaa aggcgagtgg ctgctcttca ggatgaaggg ccggagcgag
     3601 cgcggtcgcc cgccgtggct cttgcagaag ctcagggacg agttcgccgt cgcgggccac
     3661 gaggcggaag tggtcggcga agtgaaaagc aggaaggcgg tcgcgaagcc cgcgacgaag
     3721 aagaaggcgg gtgcgaagaa gcgcgcgacg aagaagcgcg cgaaggccgg agggcgtaaa
     3781 agttcttgag tccggacagg cggcagcacc gcggcgcgca cccggaggac gcgcgcctgt
     3841 tcgaggacgc gcgactgccc tcgctgcggc gcgcgaccgg cgagctgtct tggctgctga
     3901 gccgcggcta ccggctcgcg tcctcgctga agctcgtcgg cgaccgccac gcattgcgcg
     3961 agcgacagcg gctcgccgtc agccgcgccg cctgctcgga cgacagcctc gaagggcgct
     4021 ccgcgcgccg ggtcgggcct gagagcgtgc gcggcgccga gcttgtcgtt gacggcttca
     4081 acgtcgtcat caccgcggag gccgcgctcg gcggcggcgt gctcgtcagg tgccgcgacg
     4141 ggtgcgtccg cgacatgtcg agcgtccacg gctcttaccg ttcggtggac gagacggagc
     4201 gcgccctcct cgtcgtcggc gaggcgctcg aacgtctggg gccgcgctcg gtcgagtggc
     4261 tgctcgaccg cccggtctcg aacagcgggc ggctcaccgc gaggatcgag caactcgccg
     4321 ccgcgcgcgg ctggccgtgg acggtggccc ttgagttcaa ccccgaccgc agcatcgtcg
     4381 cctccccgcg catcgccgtc acctccgact cgaacatcct cgacggcgcg cgcgcgtggc
     4441 tcaacttcaa cgactacctc gtcgagcgcg acctgccgca ggcgtggctc atcgacttac
     4501 aggcgagcat tgggccgtct tgaccacgcc gcatcgttcg gcgccgggcc gcggtcgtga
     4561 gggcgctgtc gcgtagcttc cgtcgcctaa cagcttcttc aaaacccagt cggccatacg
     4621 aggcgggacg gcattgccga tcagaccgta ctggtcgtgg acggagaccg ctttgcaatg
     4681 atcgtcgggg aacccctgta ttctcgcgta ttccgtattc gtcaaacggc gataccgctc
     4741 gcccactttg taacgctcgt agtggcggct ggtcgtggcg gtcagcgtag gggcgacccc
     4801 gtcgatgccg aaaaccgtgt agcccatccg tgccccgcca ctctgattgc tgagtatctc
     4861 gacgccgtcg atgtaccgca tgacgggttt gctccccttc agcctttcca aagtcgattc
     4921 ggtgaaatcg aagtcggaag acacttccgt ttccagtacg gaacggagag gcgtgagagg
     4981 agaccgctcg gaaaaagtcg agagggtcgc ggcgaagaat ctgccttgag aggcaatccc
     5041 ccaagcaggg attcgttggc cgtgctcggc gatgcccttc cagtcggcgg ggtgcagcaa
     5101 ttcatccaat cgattccctg acagctcgct caaatcctct ttcgtggcaa gccgtatggg
     5161 aggcgacccg tgcgacggat acttgttccg atggacgcct atcagaaaga ccctctgccg
     5221 gttctgcggc aggccgaaat gcatcgcgtt gagcagtcgc cattcgatcc agtaatcgag
     5281 gtctgccagc accgataaga tggtcgcgaa gtgcgacccc tgctccatca ggagcagacg
     5341 cttgacgttc tccaagacga agtacgtcgg ctggtggcgc gccaggatcg tcgcgatggt
     5401 ctggaacaga gtgccgcgcg aatctctgat gccctctttt ttgccggcgc tgctgaacgg
     5461 ctggcagggg aaaccggcgg tcaggaggtc gtgcggaggg atggtgttcg tcaggccgtt
     5521 gacgttgcct tcgcgcaaca cggaatcgcc gaaagcatct cgatagaccg tacatgcttt
     5581 cggcgagttg tcgttcgccc agacggtctt gaagccacac ctgtccgcgg cgatgcggaa
     5641 gccgccgatg cctgcgaaaa gctcaaccgt tctcgactct tccattgccg gattccgagg
     5701 gccttaacag ccgctccctg actgccagaa ttttatcgac gcatttttcg gggtctcgtt
     5761 tgatgtcggt tccccagaga tgcaagaccc gccatccgag cgcgcgcagc ttcctgtctt
     5821 tctcgcggtc gcgctcgatg ttccgtttga ttttcgcctt ccagaagtcg tcgagcttgc
     5881 cttcccacgc cttcaggttt cggccatgcc agaaatcccc gtcgatgaaa acggcgagct
     5941 tgcagcgtct gaaaactatg tcgggtcttc ccggtaaggc gctggcgtga gtggcgaaat
     6001 atacccgctt gcgtttcaga agtcgaaaca cgattcgctc gatccccgtg ttcttcgcac
     6061 ggattttgga catgtgttcg cttcttttcg gtctggataa gaagtcgccc atctgctcag
     6121 gatgctccgt ggcggttcgt ggcggcaact cctcggcggc tcatggcggg cagtctacat
     6181 catcgcactg tgggactaca gacccgtcgt gtctgcctgt acggtcgccg caatcgttcg
     6241 cccgcgtacg gagcgggttc ggtaagtcct ttgtgaatgc gaattgcaag ccacctttgt
     6301 tgcagcgggc ggggccgatg gcgaagtcga agaaagcgcg gagattggac gtgctgagcg
     6361 cgcaggtgcg cgtctgcacg cgctgcccgc tgcacgagtc gcgtacacac gctgtaccgg
     6421 gcgatggcaa gtacgcggcg aaggtgaagg aagactgcgc tttgctcaag cgcgagttga
     6481 agaagctgtg agcgatgggg cttgagacct accggaagat gcgcgacttc aggcggacgc
     6541 cggagccgaa gggcgaggtg tcgcgcgcga accgccggcg gttcgtcgtg caggagcacc
     6601 acgcgtcgaa gctgcacttc gatttccgtc tggagatggg cggcgtgctg aagtcgtggt
     6661 cgatcccgcg cgggccttcg ctcgaccccg cggagcggcg cctcgcggtg ccgaccgaag
     6721 accacccggt cgagtacctg aaattcgaag ggcagattcc ggagggcgag tacggcgcag
     6781 gtcgtcacct gaagtgggac gcgggcacct acgaactcgt cgaaggcgac gacccgctcg
     6841 cacaactcgc cgcgggcaaa ctcaccttcg agttgaaggg gcgcaaactg cgcggctctt
     6901 tcagcctcgt gcggacgcac ctgcggggcg ccgacgagag ctggctcctc ttcaagcgcg
     6961 aggacgaata cgcggagccg ggctgggtgc ttgagctgcg gcagccaatc gacgggcagc
     7021 ggacgcttgc gaacaggaag cgcgcgaagg cggatgagag gccgcgcggc gaggcgcaca
     7081 aagaggtcag gaggttcaag accggcgcgg gcggcggcaa ggtcgtcccc gtcagcagcg
     7141 ctctcaagaa gaaagaagtg ggcggcaacg tgaacgtgaa ggtcgggcgc gaggtggtgc
     7201 cgctcaccca tctcgaccgc gtctactggc cggacgaggg cttcaccaaa gcagacctcg
     7261 tcaggtatta cgacgaggtc tccgcataca tcctgccgta cctcgaagac cgcccgctca
     7321 tcatgaagcg ctatcccgca ggcatcggcg gcccgagctt ccaccagcac gacgtgaacg
     7381 aagcgccgga gttcgtccgc acggtgcgtc accgcgcgac cgaagacggg cacgaggtcg
     7441 attacatcgt cggcggcgac cgcgcgacga acctctacat ggccaacctc ggcgccatcg
     7501 agcgccatcc gtggcactct cgcgtcggac acctcgaaca cccggactgg ttcgtcttcg
     7561 acctcgaccc cggcgcgggc gtccccgccg agaccatctg cgaggtcgcc gtcgcggtgc
     7621 gcgacgtgct ggagcgcatc gggctgaaga gctacgcgaa gacctccggc tcgcgcggca
     7681 tacacgtcta tgtccccgtc aagtccgtct atggttacga agaggtggcg gggctggccg
     7741 agcaggtggc gacgattgtc gcgcgcgaga aggcggacgt ggcgacggtc gagcgcgcgc
     7801 tggcgaagag gaagcgcggg cagatttacg tcgatcacat gcagaacgcg cgcggcaagt
     7861 cggtcgtcgc gccgtactcg gtgcgcccga agccgggcgc gcccgtctcg gcgccgctcg
     7921 aatggcagga ggtcgagcgc aaaaaaatca cgccgcggca gttcgacatc acgaacatgg
     7981 ggcggcggct cgcgagcgag ggcgacctct tccgcgcggt gctcacgaag cggcagtcgc
     8041 tcggcgcggc gttcgaggcg gcgaagaagt tatacgaaga agagcgggcc gggcgcgcgc
     8101 gcgttcgacc cgcggcggcg aacggaagaa gaaacggcag tcaatcgtcg tgaaccgcga
     8161 cgcacgaaga gggggtgaac ttatgccgag gggcgacaag tcgaagtac
//
LOCUS       DBNGOH010000413         1568 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4645811, whole genome shotgun sequence.
ACCESSION   DBNGOH010000413 DBNGOH010000000
VERSION     DBNGOH010000413.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1568)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1568)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1568
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4645811"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(70..240)
                     /locus_tag="AC159P_08120"
     CDS             complement(70..240)
                     /locus_tag="AC159P_08120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695457.1"
                     /translation="MPTETPPPYFPQNMLSPGDPKTARRMRLAFWGCAALARLGVVGL
                     VAGVVYLIRSLM"
     gene            409..918
                     /locus_tag="AC159P_08125"
     CDS             409..918
                     /locus_tag="AC159P_08125"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012632885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HIR3695458.1"
                     /translation="MIRPFKGKHPRVHPTAFVEESAQVIGDVVIGEESSVWFNAVVRG
                     DVFHVRIGARTNVQDGTVIHVSSGTHATILEDEVTVGHNVTLHGCHVERGSLVGIGAI
                     VMDGARVGAESLVAAGSLVTPGTIIPPRSLVMGVPAKVKRPLTDEEAAGLAHFWKNYV
                     EYTKEYKNE"
     gene            1038..>1568
                     /locus_tag="AC159P_08130"
     CDS             1038..>1568
                     /locus_tag="AC159P_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003960770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HIR3695459.1"
                     /translation="MKTQPARGMRDFLPTEVRRREYVVGVVREVYERYGFEPLETPAV
                     ENIETLLGKYGEEGNQLIFKILKRGEHEKTGEADLALRYDLTVPLARVVAEYGEKLPK
                     FFKRYQIQPVWRADRPARGRYREFYQCDVDAIGSRSVVVEAEIIAAACEVLERLGFDN
                     FTVRLNHREVLAGVLEA"
BASE COUNT          293 a          500 c          511 g          264 t
ORIGIN      
        1 tcattgttca cacgcggcgg cgcgcggcag gctcaccgcg cggcttaccg aaccggcagg
       61 gttacagggt tacatgagcg acctgatcaa atagaccacg cccgcgacga ggccgacgac
      121 gccgagccgc gccagcgccg cacagcccca gaacgcgagc cgcatcctgc gcgcagtctt
      181 cgggtcgccg ggcgacaaca tgttctgcgg gaagtacggc ggcggggtct cggtcggcaa
      241 cgttcacagg ctcccttctc acgcgagata ttaacaggac gcggcgcgcg cgcaaaacaa
      301 caccgcgccg cgcgtcgtgc ttgcccgccg ctcgcgcgcc gtgctacgtt tttgctttcg
      361 gcagagaccg gcggcgcaga ccgtgtttag ccatcttcgc gtggagacat gattagaccg
      421 ttcaagggca agcacccgcg ggttcacccg acggccttcg tcgaggagag cgcgcaggtc
      481 atcggcgatg tcgtcatcgg cgaggagtcg tccgtctggt tcaacgccgt cgtccgcggc
      541 gacgtgttcc acgtccgcat cggcgcgcgc accaacgtgc aggacggcac cgtcatccac
      601 gtctcctcgg ggacgcacgc caccatcctc gaagacgaag tgacggtcgg ccacaacgtc
      661 acgctccacg gctgtcacgt cgagcgcggc tcgctcgtcg gcatcggcgc catcgtcatg
      721 gacggcgccc gcgtcggcgc tgaatctctg gtcgccgccg gctcgctcgt cacgcccggc
      781 accatcatcc ccccgcgctc gctcgtcatg ggcgtgcccg cgaaggtgaa acgaccgctg
      841 accgacgaag aggccgccgg cctcgcccac ttctggaaga actacgtcga gtacacgaag
      901 gaatacaaga atgagtaggc agtaggcagc gggcagtagg cagttaagaa aacgcgtagc
      961 tcggatttga aaattcgaat cccaaaattc tcaacttgct ttcactgcct actgtctact
     1021 gcctactgcc tactgttatg aagacacaac cggcgcgggg gatgcgggac tttctgccga
     1081 cggaggtgcg gcggcgcgag tacgtcgtgg gcgtcgtgcg ggaggtctac gagcgttacg
     1141 ggttcgagcc gttggagacg ccagcggtcg agaacatcga gacgctgttg gggaagtacg
     1201 gcgaagaggg caaccagctc atcttcaaaa tcctgaagcg cggagaacat gagaagaccg
     1261 gtgaagccga tctcgctttg cgttacgacc tgacggtgcc gctggcgcgc gtcgtcgcgg
     1321 agtacggcga gaagctgccg aagtttttca agcgctacca gattcagccc gtgtggcgcg
     1381 cggacaggcc ggcgcgcggt cgttaccgcg agttctacca gtgcgacgtt gacgccatcg
     1441 gctcgcgctc ggtcgtcgtc gaggctgaaa ttatcgcagc ggcgtgcgag gtgctggaac
     1501 ggctcggctt cgacaacttc accgtgcggc tgaaccaccg cgaggtgctc gcgggcgtgc
     1561 tcgaagcg
//
LOCUS       DBNGOH010000414         3338 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_5012853, whole genome shotgun sequence.
ACCESSION   DBNGOH010000414 DBNGOH010000000
VERSION     DBNGOH010000414.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3338)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3338)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3338
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_5012853"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..757
                     /gene="pgl"
                     /locus_tag="AC159P_08135"
     CDS             <1..757
                     /gene="pgl"
                     /locus_tag="AC159P_08135"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007220439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HIR3695460.1"
                     /translation="CVGVDEGAVLQLVENLRRDAAADEEAARRFVELAAEAIADHGAF
                     GVALAGGSTPKRIYELLATDDEFKDKVDWGNTHVFFGDERTVPHDHADSNFRMAHEAL
                     LARVPVPAQNVHPMRGTGDAAANASLYEDDLRSFFGGAEWPRFDLVMLGMGDDGHTAS
                     LFPSTEALKESRAWVVSNWVEKFDTWRITLTAPAINHARHVLFTVTGAAKTDRLREVL
                     YGPSDPERLPSQSIQPAAGTLEWFVDKAAAAKL"
     gene            921..>3338
                     /locus_tag="AC159P_08140"
     CDS             921..>3338
                     /locus_tag="AC159P_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGH1-like glycoside hydrolase domain-containing
                     protein"
                     /protein_id="HIR3695461.1"
                     /translation="MPNKEQDRLREPFTTPIAPWRRWGPYVSERAWGTVREDYSPYGT
                     AWDYFPHDLARSKAYRWGEDGLGAICDRYQLLCFALALWNGRDPILKERLYGVVASEA
                     NHGEDVKEYYFYLDSTPTHSYMKYLYKYPQAEFPYSKLIDENRARRGRGPEYELLDTG
                     AFDEDRYFDVFVEYAKADAEDICIRIEAFNRGPEDAPLHVLPHLWFRNTWAWWPEPRP
                     EPSIEAGASGEGFVSLTASDEAAGPLPGLPFDYQLGTRHLYAEAGGQPLFTFNETNVP
                     RVWGGAESRKPYVKDAFHRHVVNGEPSVNPAQTGTKACVHYELNVPAGGSVVLRLRLT
                     DKVLDNPLGEVDEIVSERKGEADEFYADIHPKSASEDEKLVQRQALAGMLWTKQIYIY
                     DVNVWFDGDNPAWPPPESRRNVRNKHWLHLNSMRVLSMPDKWEYPWFAAWDLAFHCVP
                     LALVDPEFAKEQLWLLLFEQFQHPNGQIPAYEWEFSDLNPPVHAWAVWRVYNMDRVRS
                     GKSDRLFLEKCFHKLLINFAWWVNKVDSQGNNVFEGGFLGLDNITVVDRSHELPHGAR
                     LEQSDATGWMGMFCLNLMRIALELAKENKAYEGLATKFFQHYVYVGAAMKHMGGADVQ
                     LWDERDKFFYDVLRYQDGSFHKFRVRSLVGLIPLYAIERLEEDWIKPFPDFSRDLEWF
                     LENRQDIVQRCVTTIVQNGKKVHVLAIVNPDQMRHLLERVWDPTEFRSDYGLRSLSKF
                     HENNPFRFGDAGVGYEPAESVEMLKGGNSNWRGPVWFPTSFMMIESLRKLGKAFGQDF
                     EINNPVEG"
BASE COUNT          603 a         1098 c         1072 g          565 t
ORIGIN      
        1 ctgtgtaggc gttgatgaag gcgccgtcct tcagcttgtc gagaatcttc ggcgcgatgc
       61 cgccgccgac gaagaggccg cccgccgctt cgtcgagctt gccgccgagg ccatcgcaga
      121 ccacggcgcc ttcggcgtcg cgctcgcggg cggctccacg cccaagcgga tttacgaact
      181 gctggcgacc gacgacgagt tcaaagataa agtcgattgg ggcaacacgc acgtcttctt
      241 cggcgacgag cgcacagtgc cgcacgacca cgccgacagc aacttccgca tggcgcacga
      301 ggcgctgctc gcgcgcgtcc ccgtcccggc gcagaacgtc cacccgatgc ggggtacggg
      361 cgacgccgcg gccaatgcga gcctctacga agacgacctg cgctcgttct tcggcggcgc
      421 agagtggccc cgcttcgacc tcgtcatgct cggcatgggc gacgacggcc acaccgcctc
      481 gctcttcccc tcgaccgaag cgttgaaaga atcgcgcgcg tgggtcgtct ccaactgggt
      541 cgagaagttc gacacctggc gcatcacgct gaccgcgccc gccatcaacc atgcgcgcca
      601 cgtcctcttc accgtcacgg gcgcggcgaa gaccgaccgg ctgcgggaag tgctctacgg
      661 cccgagcgat ccggagcgcc ttccctcgca gtcgatccag cccgccgccg ggacgctcga
      721 atggttcgtc gataaagccg cggctgccaa actctgaaag cgggggccgg gtgtcggggg
      781 ccgggggccg gggaagaacg ggtgatgggt tctgggtttt aggtgctggt taagacattc
      841 gctttcgctg acacccaaga cctaacaccc tgcttttccc cgacacccgg ccccccatca
      901 cccggcccct cttcttgaat atgccaaaca aagaacaaga cagactccgc gagcctttca
      961 ccacgcccat cgcgccgtgg cgccgttggg ggccgtacgt ctcggagcgc gcgtggggca
     1021 cggtgcgcga ggactacagc ccttacggca cggcgtggga ctacttcccg cacgacctcg
     1081 cgcgctcgaa ggcttatcgc tggggcgagg acgggctggg cgcgatctgc gaccgctacc
     1141 agcttctctg cttcgcactc gcactctgga acgggcgcga cccgatcctg aaggagcggc
     1201 tgtacggcgt cgtcgccagc gaggccaacc acggcgagga tgtgaaggag tactacttct
     1261 atctcgactc gacgccgacg cactcgtaca tgaaatacct gtacaagtac ccgcaggccg
     1321 agtttcctta ttcgaagctg atcgacgaga accgcgcgcg gcgcgggcgc ggcccggagt
     1381 acgagctttt ggacacgggc gccttcgacg aagaccgcta cttcgacgtg ttcgtcgagt
     1441 acgcgaaggc cgacgccgaa gatatttgca ttcgcatcga agccttcaac cgcgggccgg
     1501 aggacgcgcc gctccacgtg ctgccgcacc tctggttccg caacacgtgg gcgtggtggc
     1561 ccgagccgag gcccgagccg tccatcgagg cgggcgcgtc cggcgaaggc ttcgtcagcc
     1621 tcaccgcctc ggacgaagcg gcggggccgc tccccggtct gcccttcgat taccaactcg
     1681 gcacgcgcca cctctacgcc gaagcgggcg ggcagccgct cttcaccttc aacgagacga
     1741 acgtcccgcg cgtctggggc ggcgcggaga gccgcaagcc ttacgtcaag gacgccttcc
     1801 accgccacgt cgtcaacggc gagccttcgg tcaaccccgc gcagacgggc acgaaggcgt
     1861 gcgtgcatta cgagctgaac gtcccggcgg gcggctcggt cgtcctccgt ctgaggttga
     1921 cggataaagt gctcgacaac ccgctcggcg aagttgacga gatcgtctcc gaacgcaagg
     1981 gcgaggccga cgagttttac gccgacatcc atcccaagag cgcgagcgaa gacgagaagc
     2041 tggttcagcg gcaggcgctc gcggggatgt tgtggacgaa gcagatttac atctacgacg
     2101 tgaacgtctg gttcgacggc gacaaccccg cgtggccgcc gcccgagtcg cgccgcaacg
     2161 tgcgcaacaa gcactggctt cacttgaact cgatgcgcgt gctctcgatg cccgacaagt
     2221 gggagtaccc gtggttcgcc gcgtgggact tggcgttcca ctgcgtcccg ctcgcgctcg
     2281 tcgatcccga gttcgcgaag gaacaactgt ggctgctcct cttcgagcag ttccagcacc
     2341 cgaacgggca gataccggcg tacgagtggg agttctcgga cctcaacccg ccggtgcacg
     2401 cgtgggccgt gtggcgcgtc tacaacatgg atcgcgtccg ctcggggaag agcgaccggc
     2461 tgtttctcga aaagtgtttt cacaagctgc tcatcaactt cgcgtggtgg gtgaacaagg
     2521 ttgacagtca gggcaacaac gtcttcgagg gcggcttcct cggtctcgac aacatcacgg
     2581 tcgttgaccg cagccacgag ctgccgcacg gcgcgcggct cgaacagtcg gacgccacgg
     2641 gctggatggg gatgttctgt ctcaacctca tgcgcatcgc gcttgagctg gcgaaggaga
     2701 acaaggctta cgaggggctg gcgacgaagt tcttccagca ctacgtctac gtcggcgcgg
     2761 cgatgaagca catgggcggg gcggacgtgc aactgtggga cgagcgcgac aagttcttct
     2821 acgacgtgct gcgctaccag gacggctcgt tccacaagtt tcgcgtgcgc tcgctggtcg
     2881 gcctcatccc gctctacgcc atcgagcgcc ttgaggagga ctggatcaag ccgttccccg
     2941 atttcagcag agatcttgag tggttccttg agaaccggca ggacatcgtg cagcgctgcg
     3001 tgacgaccat cgtgcagaac ggcaagaagg tgcacgtgct ggccatcgtc aaccccgacc
     3061 agatgcggca tcttttggag cgcgtgtggg acccgacgga gttccgctcg gactacgggc
     3121 tgcggagcct ctcgaagttt cacgagaaca acccgttccg cttcggcgac gcgggcgtcg
     3181 ggtacgagcc ggcggagtcg gtcgagatgt tgaagggcgg caactcgaac tggcgcggcc
     3241 ccgtctggtt cccgacctcc ttcatgatga tcgagtctct gcgcaagctc ggcaaagcct
     3301 tcgggcagga cttcgagatc aacaacccgg tcgagggc
//
LOCUS       DBNGOH010000415         4204 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4096777, whole genome shotgun sequence.
ACCESSION   DBNGOH010000415 DBNGOH010000000
VERSION     DBNGOH010000415.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4204)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4204)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4204
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4096777"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(25..1788)
                     /locus_tag="AC159P_08145"
     CDS             complement(25..1788)
                     /locus_tag="AC159P_08145"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024583.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3695462.1"
                     /translation="MAEDKDIPRGGAAGEAEEPPRAATLFDVGPVGEANRAETAAALR
                     AAVETVRLIVGADTASVASFSEEERTITWHAMSGFETLDADAGEIVTSLRGEFAERAA
                     GAGDNFIEVRGVAGDLPASEFPVHSAEGVRSLALVRLVARGETLGVLSVGYRAAHRFT
                     AEERQRLEGLADMVALALDNARLVETIGTAKRVWEQTFDAIPDGIIVHDDRMRIARCN
                     YTAAELMGFVRPAEAIGMSCADAFARLFGERAAAYHMRQGGIRKISAFELQAEDGRHY
                     LVSVAPITSPESGVRSPESEQEASISEAEEQAASTEHQTSDSGLRTPDSGLRTPSWSV
                     IMWSDITELSEVREQMARTRRLATIGQLAAGVAHEINNPLAAITTCAEATLRDLREAP
                     ETAPHVAERNWDFYLEEIVRQTLRCKQITRGLLDLSRQRRPKRVSVELNKVVAQCAKL
                     FERRGSERGIDLAVRLDEAVGEVATDEAMVCQVLDNLLSNALDAVEGGGRISVATALD
                     GARVAVEVADDGPGIPPETLARIFDPFFTTKDPGSGSGLGLAISLTLAEAMGGALTVE
                     SKPGAGSRFRLWLPRRTPEKQ"
     gene            2179..2775
                     /locus_tag="AC159P_08150"
     CDS             2179..2775
                     /locus_tag="AC159P_08150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695463.1"
                     /translation="MRFDFEMQDERRRHVMKRALEALGWLAAICLFFFAGHAVLAQDA
                     RGGEPAAQQQQQNRGRRAGERRDARDATPASIMREARTVFVRETRHLDKVYLEYKLQK
                     YNELREWELMLVDREDAADLVIQIEKTALNYIFTIVDPRTRAIVASGKTVAINGLVAA
                     ENLGREIIKKIRDVRAGPDRKGRRKKPHDEDEDEWSES"
     gene            2842..3450
                     /locus_tag="AC159P_08155"
     CDS             2842..3450
                     /locus_tag="AC159P_08155"
                     /inference="COORDINATES: protein motif:HMM:NF024895.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HIR3695464.1"
                     /translation="MRKELLFSLLSLALLATGAARAQSSSPVEEHKFEVGALYTAINL
                     GDLGDPVSGLGGRVAYNFNEHFALDAEASFFPRTHLGNSEFGQKTQGFVGVRAGARSK
                     YFGVFAKARPGVMFIGEVTSGFDCNSQPFGQVCRPEHNHFAFDAGGVFEFYPSSRAII
                     RVDAGDTIVRVNSATRGVFTGPQSSSDVTHNFQISVGVGYRF"
     gene            3480..>4204
                     /locus_tag="AC159P_08160"
     CDS             3480..>4204
                     /locus_tag="AC159P_08160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695465.1"
                     /translation="MKVRALITLLLICCLGATHASAGARFAERGAREQLTSEEEREAR
                     EVANAIFKRLEEEQDIAPLLEEMFVRDFAARLHASVSEAGSLYLLDRTVATQASRDEV
                     LRHHVSVSNLILLTSLYMTNARLNARAERRAARANEQGREGKAEEEGATEEEEETRED
                     PSKLADIFPPEVLELFRADPLIGRFVIEQETREAREAAPQEAAPGDASAAAQADTGSD
                     EEEFISTVEQLRAFTSTVERANEL"
BASE COUNT          693 a         1449 c         1404 g          658 t
ORIGIN      
        1 tgcctgctgc ccgctgccta ctgcctactg cttttccggc gtgcggcgcg ggagccagag
       61 gcggaagcgg ctgccggcgc cgggcttgga ttcgacggtg agcgcgccgc ccatcgcttc
      121 ggcgagcgtg agcgagatgg cgaggccgag gccggagccg gagccggggt ccttggtggt
      181 gaagaagggg tcgaagatgc gcgcgagggt ttcgggcggg atgccggggc cgtcgtcggc
      241 gacctcgacc gcgacgcgcg cgccgtcgag cgcggtcgcc accgagatgc gcccgccgcc
      301 ctcgaccgcg tcgagcgcgt tgctcagaag gttgtcgagc acctgacaga ccatcgcctc
      361 gtcggtcgcc acctcgccca ccgcctcgtc gagccgcacc gcgaggtcga tgccgcgctc
      421 gctgccccgc cgctcgaaga gcttcgcaca ctgcgcgacc accttgttca gctcgacgct
      481 cacgcgcttc gggcgccgct gccgcgacaa atcgagcagc ccgcgcgtga tctgcttgca
      541 gcgcaaggtc tgccgcacga tctcttcgag gtagaaatcc cagttgcgct ccgcgacgtg
      601 cggggccgtc tcgggcgcct cgcgcaggtc gcgcagggtc gcctcggcgc aggtcgtgat
      661 ggcggcgagc gggttgttga tctcgtgcgc gacgcccgcc gcgagctgcc cgatggtggc
      721 cagacggcgg gtgcgcgcca tctgctcgcg cacctccgaa agctccgtga tgtcgctcca
      781 catgatgacg ctccacgaag gagtccggag tccggagtcc ggagtccgga gtccggaatc
      841 ggaggtctga tgctcggtgc tcgccgcttg ctcttctgcc tccgagattg aagcctcttg
      901 ttcagactcc ggactccgga ctccggactc cggactcgtg atgggcgcga ccgagacgag
      961 gtagtgtctg ccgtcctcgg cctgtagctc gaaggcgctg atcttgcgga tgccgccctg
     1021 ccgcatgtgg taggcggcgg cgcgctcgcc gaagagacgc gcgaaggcgt cggcgcacga
     1081 catgccgatg gcctcggcgg ggcgcacgaa gcccatcagc tccgccgccg tgtagttgca
     1141 gcgcgcgatg cgcatgcggt cgtcgtggac gatgatgccg tcggggatgg cgtcgaaggt
     1201 ctgctcccag acgcgcttgg cggtgccgat ggtctcgacg agccgcgcgt tgtcgagcgc
     1261 gagcgcgacc atgtcggcca gcccttccag acgctgccgc tcctccgccg tgaagcggtg
     1321 cgccgcgcgg tagccgaccg agagcacgcc cagcgtctcg ccgcgcgcga ccagtcgcac
     1381 gagcgcgagc gagcggacgc cctccgcgct gtggacggga aactcgctcg ccggcaaatc
     1441 gcccgccacg ccgcgcacct cgatgaagtt gtcgcccgcc cctgcggcgc gctcggcgaa
     1501 ctcgccgcgc agcgaggtga cgatctcgcc cgcgtccgcg tccagcgtct cgaagccgct
     1561 catcgcgtgc cacgtgatcg tccgctcctc ttccgagaag ctggcgacgc tcgccgtgtc
     1621 ggcgccgacg atgagccgca cggtctcgac cgccgcgcgc aaggccgccg ccgtctcggc
     1681 ccggttcgcc tcgcccacag gccccacgtc gaacagcgtc gccgcgcgcg gcggctcctc
     1741 cgcttcgccc gccgcgcccc cgcgcggaat gtccttgtcc tctgccatcg tccctttatc
     1801 ttgccataaa gtaggcagta ggcagtaggc ggtaggcagt ttgaagatag gagtccggtg
     1861 tctggagtca ggagtcggaa gaggctcggg aggtacagtc ccgcgcctcc ccgctcttcg
     1921 agcgatgacg caagaggctc ggcactttac ctcccgagcc tcttcgtctt tccgacttcc
     1981 gacttccgac ctctgtcttc tgtctttcac tgcctaccgc ctaccgccta ctgcctactg
     2041 cttccttgtc gcaaatatct ttcacctgac gcggcggcgc ggcgcggagt tgttgcgacg
     2101 cgcggcggaa acccgcgttt ttgcggcgcg cgggcgacgg cacggtcgtt gccaacgatg
     2161 tgggcgacga ggggtgacat gcgattcgat ttcgagatgc aggacgaacg gaggagacac
     2221 gtgatgaaaa gggcgctcga agcgttgggc tggctcgccg ccatctgcct cttcttcttc
     2281 gccgggcacg ccgtgctcgc gcaggacgcg cgcggcggcg aacccgcggc gcagcagcag
     2341 cagcagaacc gcgggcgccg cgcgggcgag cggcgcgacg cgcgcgacgc cacccccgcc
     2401 tcgatcatgc gcgaggcgcg caccgtcttc gtccgcgaga cgcgccacct cgacaaggtc
     2461 tacctcgaat acaagctgca aaagtacaac gagctgcgcg agtgggagct gatgctggtg
     2521 gacagggagg acgccgcaga cctcgtcatc cagatcgaga agaccgcgct caactacatc
     2581 ttcaccatcg tcgatccgcg cacgcgcgcc atcgtcgcga gcggcaagac ggtcgccatc
     2641 aacggcctcg tcgccgccga aaatctgggc cgcgagatca tcaagaagat cagggacgtg
     2701 cgcgccggcc ccgaccgcaa gggccgccgg aagaagccgc acgacgaaga cgaggacgag
     2761 tggagcgagt cgtaaacatt ccgcaggcgg actgaagcga ggcgtcgaac actcaggaca
     2821 ggaatgctca cggaggatgt catgcgaaag gaacttctgt tttcactcct ctcccttgcc
     2881 ctgctcgcga ccggcgcggc gcgcgcgcaa agctcgtcgc ccgtcgagga gcacaagttc
     2941 gaggtcggcg cgctctatac ggccatcaac ctcggagacc tcggcgaccc cgtctccggc
     3001 ctcggcggtc gcgtcgccta caacttcaac gagcacttcg cgctcgacgc agaggccagc
     3061 ttcttccccc ggacgcacct cggcaacagc gagttcgggc agaagacgca ggggttcgtc
     3121 ggcgtcaggg cgggcgcgcg ctcgaaatac ttcggcgtct tcgccaaagc gcgccccggc
     3181 gtcatgttca ttggcgaagt cacgtccggc ttcgactgta actcgcagcc cttcgggcaa
     3241 gtctgccgcc ccgagcacaa tcacttcgcc ttcgacgcgg gcggcgtctt cgagttttat
     3301 ccttcgtcgc gcgcgatcat ccgcgtggac gcgggcgaca ccatcgtccg cgtgaacagc
     3361 gccacgcgcg gcgtcttcac aggcccgcaa agttcgagcg acgtgacgca caacttccag
     3421 atcagcgtcg gcgtcggcta ccgcttctga gccgcgcggg gcatgaggtt caaagagcca
     3481 tgaaagtacg cgctctcatc acactgctgt taatctgctg cctcggcgcg acgcacgcct
     3541 cggcgggcgc acgcttcgcc gagcgcggcg cgcgcgagca actcacgtcg gaggaggaac
     3601 gcgaggcgcg cgaggtcgcc aacgcgatct tcaagcggct tgaggaagag caggacatcg
     3661 ccccgctgct cgaagagatg ttcgtcagag acttcgccgc gcgcctgcac gcctcggtca
     3721 gcgaagccgg ttcgctctac ttgcttgacc gcaccgtcgc cacgcaggcg agccgcgacg
     3781 aggtactccg ccatcacgtc tccgtcagca accttatcct cctcacctct ctctacatga
     3841 cgaacgcacg gctcaacgca cgcgccgaga ggcgagccgc gagggcgaac gagcagggca
     3901 gagagggcaa ggcggaagag gagggagcga cggaagaaga ggaagagacg cgagaagacc
     3961 cctcgaagtt ggccgacatc ttcccgccgg aggtgctcga acttttcaga gccgacccgc
     4021 tcatcggccg cttcgtcatc gaacaggaaa cgcgtgaagc gcgcgaggcg gcgccgcaag
     4081 aggccgcgcc gggcgacgcg agcgcggcgg cgcaggcgga tacgggttcg gacgaggagg
     4141 agttcatctc gacggtcgag cagttgcgcg cgttcacttc gacggtcgag cgcgccaacg
     4201 aact
//
LOCUS       DBNGOH010000416        10528 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2448053, whole genome shotgun sequence.
ACCESSION   DBNGOH010000416 DBNGOH010000000
VERSION     DBNGOH010000416.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 10528)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10528)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10528
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2448053"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1553)
                     /locus_tag="AC159P_08165"
     CDS             complement(<1..1553)
                     /locus_tag="AC159P_08165"
                     /inference="COORDINATES: protein motif:HMM:NF015714.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HIR3695466.1"
                     /translation="MGAVPHRPAFSSSPLAALAAAFAAGVALARFAAPPLAACIVCGA
                     LLSVFSVYALYRKKLALASTTLLAAFVFAGVALALAETKGIAENRVRRFYERGEIASG
                     DPLELTGVLARAPEVVPDGLLVTLRVERLKHRYEERAATGSVELFAPVRDARASDGYA
                     ALELRRGARVRVMAALTRAERFRNPGVSSLTEFLEQRDVDARGHVKSHLLFERLDDER
                     VFLPLFALEEWRARLARLFEETFSAETAGVLKAALLGNRHGLSRATAERFREGGTFHV
                     LVISGMHITFVGGVVWFAARRLTRGAARQWLASVAAVWGYAVFVGAEASVVRAALMFT
                     VVALAPVLGRRTHALNALGGAGLLLLVWRPANLFDPSFQLTFLSVLSIVALALPLLQG
                     LKAVGEWRPTRSTPYPPACPRWWGALGEILFWSEKDWRREMARSAHDYRLFKTPLAAR
                     LDRWRVQRVLRYVFAATVVSMVVQLTLLPLFVVYFHRLSLVAPLLNILVGALMVLLAL
                     AALAATALA"
     gene            complement(1729..2184)
                     /locus_tag="AC159P_08170"
     CDS             complement(1729..2184)
                     /locus_tag="AC159P_08170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695467.1"
                     /translation="MLRSIAAVVVGYLVMFLLVFTLFTAAYFALGTEAAFAPGTYEVS
                     TLWVVISFVLGLVAALVGGYVCAAIARRGKAPLALAALVLGLGILFAIPVLTAPKESA
                     PRAGGVSNLEAMQNARMPGWVALLNPFVGAVGVLLGARLKRGPVESTTA"
     gene            2498..3727
                     /locus_tag="AC159P_08175"
     CDS             2498..3727
                     /locus_tag="AC159P_08175"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3695468.1"
                     /translation="MPFTEAIKLALASILSHKLRSFLTLLGVIFGVATVIVVVSLVEG
                     FNSYVDEKIANIGTNAFAVQKYGIADFSSVEALNAARRRNKDVTLEDLEALRRARGGA
                     IRDAGGKEQGQADLKRGATSLFRVRLVAATHNIAEIEKIEAGEGRYFTAPEESARRAV
                     CFVGADVAEKLFPTSSALGQTIRVDGRPFQVVGVGKVLGSVFGQSRDMYVTIPLTTFM
                     SIYGSRRSISISVTSTAEGTYADAIDEARVVLRTRRKLGPAAEDNFGVITPNAINELR
                     DQIFGTIQTAAIGVTSISLVVGGIVIMNIMLVSVTERTKEIGLRKSIGARRRDILRQF
                     LAESTMLSLIGGATGVTIAYLLAKLVAVATPVPTALPLLAVAAALIVSGGVGMISGVY
                     PAWKAARLDPIEALRAD"
     gene            3867..5135
                     /locus_tag="AC159P_08180"
     CDS             3867..5135
                     /locus_tag="AC159P_08180"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3695469.1"
                     /translation="MKFLRADFFENLRMALDTLRSNKLRSFLTILGVVVGTMTVIVIA
                     AFVAGIGNRVEKEIESFGTNSIYAYRFDPGFNINPSAEERMRKPLSYEDALAMKAECP
                     SVTHVAPFISPVDFLGGTFAERVNVRRRDIEMTNATVQGTLPDYFRMGVATVSEGRFF
                     GDEENTRRADVAVIGRDVANTLFPFTGAVEQEITINGRDYRVVGVLAERETFLVGAED
                     PNNENKAVYLPYLTIKKLYPDVDDNFIMAQAAPGRMNEAVEEVREVLRKRRKVPFDKP
                     DNFGISTSDQIIEQFGAITGGVFALMVAISGVGLLIGGIGVMNIMLVSVTERTREIGV
                     RKAIGARRRDIVTQFLIEAATLTGLGGVLGILLGSGIALLIQTIMPTHIPLWAPVVGF
                     AVSVGLGLGFGLWPAWKAARLNPIEALRYE"
     gene            5302..6627
                     /locus_tag="AC159P_08185"
     CDS             5302..6627
                     /locus_tag="AC159P_08185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695470.1"
                     /translation="MNNTKCPRCGLVNWSGDEVCERCGAPLAGGDPEARAPEAARPDT
                     QGLTLGSDVPTGSRTWKPVLLILVVVALGGAALAVYKWRAGAGRRSVMVQAMRKEMTP
                     PSLDERTRDVMLSYLAQPGFGGTEVVYQVLGPARLEMVQVDDREAYFSAPAPEQSGEG
                     VEATIARFAADPKRVVLAEDDGKGRLRLGDYSLPRSTDKHFFFKTPVDNLRFDPATVL
                     KFPFGRATYTLDMRELSDFLRNKSVFGGRMRAQTEQRRAGLPVVFANHGAFVARPEET
                     SLRRFVGELVRDLPADGDGARESRVQRVLDFVSREIAYDLREASYNFETLKRPNEVLM
                     TGESDCSNKAILLGSLLEQLGEDYLFVYTPDHITVAVRQGGFPARNGLSLEWEGQTWL
                     VAEGTVPGFRIGLDRVLEEARLKRFQHVQRPRDRDVIFDLATGRQLLFQ"
     gene            complement(6710..7975)
                     /locus_tag="AC159P_08190"
     CDS             complement(6710..7975)
                     /locus_tag="AC159P_08190"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017743.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HIR3695471.1"
                     /translation="MRGRKQSPRGRAAPGGASLRVGVEVEVEVERILPGGVGLAHAGG
                     QTVFVALAAPGDRALVRVARVRGAVAFASIKELLAPSPARVEPPCPYFGRCGGCDFQQ
                     LDYPAQLEAKVGIIRDCLRRIARLEPPADIPITPSPAEWHYRSRARWQHDPLRKHLGY
                     YERGTHHVIDVVECPVAAPPVQEKLTALRRRMNEDDLPDGAAEFEAVAGDRGVSLTPP
                     LGEEDAREQERAIGHERYRFGAGCFFQINHALLAPLVRAGLGDANGASALDLYCGVGL
                     FTLPLARRYARVQAVEGNAASADYARRNLSAAQLSNARVETSHVGDWLSRHTAALTSE
                     HATPRTPFDFVLLDPPRTGAEPETLDALLRLSPPHISYVSCDPATLARDLRTLTEEGG
                     YRLASLQALDMFPQTHHVETVVHLMKAVV"
     gene            complement(8130..8531)
                     /locus_tag="AC159P_08195"
     CDS             complement(8130..8531)
                     /locus_tag="AC159P_08195"
                     /inference="COORDINATES: protein motif:HMM:NF023511.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3566 domain-containing protein"
                     /protein_id="HIR3695472.1"
                     /translation="MAKMQVKRVGVFSYAKITAVTNAAFGLIIGVIYGLIFMVVGGAI
                     MAGGGRDTGAAGAGSIAVGLVIMVVFPIFTGVVGFIAGVIGALIYNVAAGFVGGLELE
                     MENADAEYAAPPPPQWSADQYQPGQPQYPYS"
     gene            complement(8573..9388)
                     /locus_tag="AC159P_08200"
     CDS             complement(8573..9388)
                     /locus_tag="AC159P_08200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695473.1"
                     /translation="MSDTQGARAARQGAARDRLNYFNYFTEIEDAFIRRRGKHLLLSP
                     MDWALMDSWKRMGVPLHVVLRGVESAFDSYESKPRKRTVKSLYYCQEEVEAQYAEWLE
                     GQRGANAKTGDAAHDAGAAGDVETNEPDEAAGLPFPRAVIAAHLAECREALARAGESR
                     RREVGEDELCEALARAAARLAELEADFARAARPDAQALEDSLTDLEALLDRAVRAPLP
                     APKLAAKRAHAAEQLEPYRARMERATYEQTLDNLLAKALREEYGVPRLSLFYL"
     gene            complement(9601..>10528)
                     /locus_tag="AC159P_08205"
     CDS             complement(9601..>10528)
                     /locus_tag="AC159P_08205"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019403.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acetyltransferase domain-containing protein"
                     /protein_id="HIR3695474.1"
                     /translation="LWLVVLEFTVVRAAVLFNFDYRELGVAQVIWVIGVSMIVLAALV
                     HLPLRVVGAFGVLLIVLHNLLDGIRVPFWQGPGTPEPSFWQAVWLVLHQQGPLPLPGG
                     TSTFIIYPLIPWVGVMAAGYAFGALYQLTPERRRRILLALGASMIAAFVLLRAFNVYG
                     DPQPWDVQSRGALFTLLSFLNTAKYPPSLLFLLMTLGPAIMALAFFERAGRRGPLARA
                     LITFGRVPLFFYVWQWLTAHALAILVGYLAGQSVAWQFANFADKPFPNPNGFDLWVVY
                     VFWAVGLVLLYPLCRWFAGVKQRRRDWWLSYL"
BASE COUNT         1790 a         3508 c         3618 g         1612 t
ORIGIN      
        1 tgcgcgagcg ccgtcgcggc gagcgcggcg agcgcgagca ggaccatcag cgcgccgaca
       61 aggatgttca gcagcggcgc gacgagcgag agcctgtgaa agtagacgac gaagagcggc
      121 aggagcgtga gctgtacgac cattgaaacg accgtggcgg cgaagacgta acgcagcaca
      181 cgctgtacgc gccagcggtc aagccgcgcg gcgagcggcg ttttgaagag tctgtagtcg
      241 tgcgccgagc gcgccatctc gcgccgccaa tccttctcgc tccagaagag aatctcgccg
      301 agcgcgcccc accagcgcgg gcacgcgggc ggatacggcg tcgagcgcgt gggccgccat
      361 tcgccgacgg ccttcaaacc ctgaagcagc ggcagcgcga gcgcgacgat cgagagcacg
      421 gagaggaacg tcagttggaa cgacgggtcg aagaggttcg ccggtcgcca cacgagcagc
      481 aacagccctg cgccgccgag cgcgttgagc gcgtgcgtgc gccgaccgag gacgggcgcg
      541 agcgcgacca ccgtgaacat caatgccgcg cggacgaccg atgcctccgc gccgacgaac
      601 actgcgtagc cccacaccgc cgcgaccgac gcgagccact gccgcgccgc gccgcgcgtc
      661 aggcgtcgcg ccgcgaacca cacgacgccg ccgacgaagg tgatgtgcat gccgctgatg
      721 acgagcacgt ggaaggtgcc gccctcgcgg aagcgctcgg cggtcgcgcg cgagaggccg
      781 tggcggttgc cgagcagcgc cgccttcaac acgcccgccg tctccgcgga gaatgtctct
      841 tcgaacaacc gcgccagacg cgcgcgccac tcttcgagcg cgaacagcgg caggaacacg
      901 cgctcgtcgt cgagccgttc gaagagcaag tgactcttga cgtggccgcg cgcgtccacg
      961 tcgcgctgtt cgagaaactc cgtcagcgaa gagacgccgg ggttgcggaa acgctcggcc
     1021 cgcgtcagcg ccgccatcac gcgcacgcgc gcgccgcgcc tcagttcgag cgccgcgtaa
     1081 ccgtccgacg cgcgcgcgtc gcgcaccggc gcgaacagtt cgacgctccc cgtcgccgcg
     1141 cgctcttcgt accgatgctt cagcctctcg acgcggagcg tgacgagcag gccgtccggc
     1201 acgacctccg gcgcccgcgc cagcacgccc gtcaactcca acgggtcgcc cgaagcaatc
     1261 tcgccgcgct cgtaaaaacg ccgcaccctg ttttcggcga tgcctttcgt ctcggcgagc
     1321 gccagcgcga cgcccgcgaa gacgaaggcg gcgaggagcg tcgtcgaagc gagcgcgagc
     1381 tttttcctgt acagcgcata gacggagaac acggagagca gcgcgccgca gacgatgcac
     1441 gccgcgagcg gcggcgcggc gaagcgcgcc agcgccacgc ccgccgcgaa ggcggcggcg
     1501 agcgccgcga gcggggagga gctgaaagca gggcgatgag gcaccgcgcc cataagtttc
     1561 tgtaaccgcc gcgagacgcc ccgccggaag ggcgcggccc ttccgcgaag cacggctaag
     1621 gttgcgagcg gcgcgagagg tcgagcttgg gcgggtcacg ccggaacgtg ttcgttacgt
     1681 cgaaaggata ttcgtcaggc cggaacgttg ttcgaatgcg ggcgccgttc acgccgtcgt
     1741 cgattcgacc gggccgcgct tcagacgcgc gccgagcagt acgccgacgg cgccgacgaa
     1801 ggggttgagg agcgccaccc agccgggcat ccgtgcgttc tgcatcgcct ccaggttcga
     1861 tacgccgccc gcgcgcgggg cgctctcttt cggcgccgtc agcacgggga tggcgaacag
     1921 gatgccgagg ccgagcacca gcgcggcgag cgcgagcggc gccttgcctc gccgcgcgat
     1981 ggcggcgcag acgtagccgc cgaccaacgc ggcgacgagg ccgagcacga agctgatgac
     2041 gacccagagc gtggagacct cgtaagtgcc gggcgcgaac gcggcctccg tgcccaatgc
     2101 gaagtaggcc gcggtgaaca gtgtgaagac gagcaaaaac atgacgagat agccgacgac
     2161 cacggcggcg atgcttctta acattagcga tgctcccttg aagagtgaac ggcgggggct
     2221 tgaatctatt acagaagacg ccgccgcgca atatctttgt tcgcccggcg cgtttcgacg
     2281 actctgtttt tacgatgttt cacgcgtgag attttgtgtt acaatagcaa ccacacgaag
     2341 cgggcacttc cgggcgagaa ggcgtccgag agtccggctc cgtcacctcg cacgcgccgg
     2401 gccgcgcttc agccgcacgc tgaaacttac gtgtgccgcg cgcgtaagat taaccttcgg
     2461 acgcgtggcc gcgctgcgcg ttcgcgcgag gtatgggatg ccgttcaccg aggctatcaa
     2521 gctggcgctg gcttcgatcc tgtcgcacaa gctgcgctcg ttcctgacgc tgctgggcgt
     2581 catcttcggc gtggcgacgg tgatcgtcgt cgtctcgctg gtcgagggct tcaactctta
     2641 cgttgacgag aagatcgcca acatcggcac gaacgccttc gccgtgcaga agtacggcat
     2701 cgcggatttc tccagcgtcg aggcgctgaa cgcggcgcgg cggcgcaaca aggacgtgac
     2761 gctcgaagac ctcgaagcgc tcaggcgcgc gcgcggcggc gccatccgcg acgcgggcgg
     2821 caaggagcag gggcaggccg acctcaagcg cggcgccacc tcgctcttcc gcgtccggct
     2881 cgtcgcggcc acgcacaaca tcgccgagat cgaaaagata gaggcgggcg agggccgcta
     2941 cttcaccgcg cccgaagagt cggcgcgccg cgccgtctgc ttcgtcgggg cggacgtggc
     3001 cgaaaagctc ttccccactt cgagcgcgct cggccagacg attcgcgttg acgggcggcc
     3061 cttccaggtg gtcggcgtcg gcaaggtgct cggctccgtc ttcggccagt cgcgcgacat
     3121 gtacgttacg atccctctga cgaccttcat gtccatctac ggctcgcgcc gctcgatcag
     3181 catcagcgtc acctcgaccg ccgaaggaac ttacgccgac gccatcgacg aagcgcgcgt
     3241 cgtcctgcgc acgcgccgca agctcggccc tgcggcggaa gacaacttcg gcgtcatcac
     3301 gccgaacgcc atcaacgagc tgcgcgacca gattttcggc accatccaga ccgccgccat
     3361 cggcgtcacc tcgatttcgc ttgtcgtcgg cggcatcgtc atcatgaaca tcatgctcgt
     3421 ctccgtcacc gagcggacga aggagatcgg cctgcgcaaa tccatcgggg cgcggcgccg
     3481 cgacatcctc cgccagttcc tcgccgaatc gacgatgctc tcgctcatcg gcggcgcgac
     3541 gggcgtcacc atcgcttatc tgctcgccaa gctcgtcgcc gtcgccacgc ccgtcccgac
     3601 cgcgctcccc ttgctcgcgg tcgccgcggc cctgatcgtc tcgggcggcg tcggcatgat
     3661 ttccggggtc taccccgcgt ggaaggccgc gcggctcgac ccgattgaag cgcttcgcgc
     3721 agactagccg ttagcgatta gctgttagcc attagctaag agctaacagc ctagccgctg
     3781 ttatgagtcg ccgtgcaaag ttgagttgag atgttttata agtgccgggt tcaagttaaa
     3841 ggctaacagc taatcgctaa aagctcatga aattcctccg tgctgacttc ttcgaaaacc
     3901 tgcgcatggc gctcgacact ttgcgctcga acaagctgcg gtcgttcctg acgatcctcg
     3961 gcgtggtcgt cggcacgatg acggtcatcg tcatcgcggc cttcgtcgca ggcatcggca
     4021 accgcgtcga gaaggagatc gagtcgttcg ggacgaactc gatctacgcc taccgcttcg
     4081 accccggctt caacatcaac ccgtccgcgg aggagcggat gcgcaagccc ctgagctacg
     4141 aggacgcgct cgccatgaag gccgagtgcc cttccgtcac gcacgtcgcg ccgttcatct
     4201 cgccggtgga cttcctcggc ggcaccttcg ccgagcgcgt caacgtccgc cgccgcgaca
     4261 tcgagatgac gaacgcgacc gtgcagggca cgctcccaga ctacttccgg atgggcgtgg
     4321 cgaccgtctc ggaggggcgc ttcttcggcg acgaggagaa cacccgccgc gccgacgtgg
     4381 ccgtcatcgg gcgcgacgtg gccaacacgc tcttcccctt cacgggggcg gtcgagcagg
     4441 agatcacgat caacgggcgc gactatcgcg tcgtcggcgt gctcgccgag cgcgagacct
     4501 tcctcgtcgg cgccgaagac ccgaacaacg agaacaaggc cgtctacctc ccttacctca
     4561 cgatcaaaaa gctctacccg gacgtggacg acaacttcat catggcgcag gccgcgcccg
     4621 gcaggatgaa cgaagccgtc gaagaggtgc gcgaagtcct gcgcaaacgg cgcaaggtgc
     4681 ccttcgacaa gccggacaac ttcggcatct ccacctccga ccagatcatc gagcagttcg
     4741 gcgccatcac cggcggcgtc ttcgcgctga tggtcgccat ctccggcgtc gggcttctga
     4801 tcggcggcat cggcgtgatg aacatcatgc tcgtctccgt caccgaaagg acgcgcgaga
     4861 tcggcgtgcg caaggccatc ggcgcccgcc gccgcgacat cgtcacgcag ttcctcatcg
     4921 aggccgccac gctgacgggc ctcgggggcg tgctcggcat cctcctcggc tccggcatcg
     4981 cgctcctcat ccagaccatc atgcccacac acatcccgct ctgggcgccc gtcgtcggct
     5041 tcgccgtctc cgtcggcctc ggactcggct tcggcctctg gcccgcctgg aaggccgccc
     5101 gccttaaccc catcgaagcc ctccgctacg agtaagcgtg gaaatattcg atcgatgttg
     5161 acgcgccttt tcgcggaagc tatattgcgc ggggcgcgcg cacgcgtgag tgaccttcac
     5221 caagccgggc gcgccgcctc acgtcttcgt taatccgaac accccgtgcc gacgcgcgcc
     5281 tcaatccctt tggagagatg aatgaataac acgaaatgtc cccgctgcgg cctcgtcaat
     5341 tggtcgggcg acgaagtctg cgagcgttgc ggcgcgccgc tcgccggcgg cgaccccgaa
     5401 gcgcgcgcgc cggaagcggc gcggcccgac acgcagggcc tcacgctcgg aagcgatgtg
     5461 ccgacgggtt cgaggacgtg gaagccggtt ctgctgatcc tggtcgtggt cgcgctcggc
     5521 ggtgcggcgt tggccgtcta caagtggcgt gccggcgcgg gtcggcgcag cgtcatggtg
     5581 caggcgatgc ggaaggagat gacgccaccg agcctcgacg agcgcacgcg cgacgtaatg
     5641 ctctcctacc tcgcgcagcc cggcttcggc gggacggaag tggtctatca ggtgctcggc
     5701 cccgcgcggc tggagatggt gcaagtggac gaccgcgaag cctacttttc ggcccccgcg
     5761 ccggagcagt cgggcgaggg ggtggaggcg accatcgcgc gcttcgccgc cgacccgaag
     5821 cgggtcgtgc tggcggagga cgacggcaag ggccggctcc gcctcggcga ctactcgctg
     5881 ccccgttcga cggacaagca cttcttcttc aagacgccgg tggacaacct caggttcgat
     5941 ccggcgaccg tcctcaagtt tccgttcggg cgggcgacct acacgctcga catgcgcgag
     6001 ctgagcgact tcctccgcaa caagagcgtc ttcggcgggc ggatgcgcgc gcagacggag
     6061 cagaggcggg cggggctgcc cgtcgtcttc gccaaccacg gcgcgttcgt cgcgcggccc
     6121 gaagagacct cgctgcgccg cttcgtgggc gagctggtcc gcgacctccc ggcggacggc
     6181 gacggcgcgc gcgagtcgcg cgtgcagcgc gtgctcgatt tcgtctcgcg cgagatcgcc
     6241 tacgacctgc gcgaggccag ctacaacttc gagacgctga agaggccgaa cgaggtcttg
     6301 atgacgggcg agtcggattg cagcaacaag gccatcctcc tcggctcgct gctggagcag
     6361 ctcggcgaag actacctgtt cgtctacacg cccgaccaca tcacggtcgc cgtccggcag
     6421 ggcggcttcc ccgcgcgcaa cggcctctcg ctcgaatggg aagggcagac ctggctcgtc
     6481 gccgaaggca ccgtccccgg cttccgcatc ggccttgacc gggtgctcga agaggcgcgc
     6541 ctcaagcgat tccagcacgt ccagcgcccc agagaccgcg acgtgatctt cgacctcgcc
     6601 acgggccggc agttgttatt ccaatagtgc gcgccgccga ccgatagaag ttccgccact
     6661 actccgactt cacgttcact cccgtgcagt cgcatccact ttcgtatttt cacaccaccg
     6721 ctttcatcag gtgcacgacc gtctcgacgt ggtgggtttg ggggaacatg tcgagggcct
     6781 ggagggaggc gagtcgatag ccgccttctt ccgtcagggt gcggaggtcg cgggcgaggg
     6841 tggcggggtc gcaggagacg taggagatgt gcggggggga gaggcggagg agggcgtcga
     6901 gggtttctgg ttcggcgccg gtgcggggcg ggtcgaggag gacgaagtcg aagggtgttc
     6961 gaggggtcgc gtgttcggag gtgagggcgg cggtgtggcg ggagagccag tcgccgacgt
     7021 gggaggtttc gacgcgggcg ttcgagagtt gtgcggcgga gaggttgcgg cgggcgtagt
     7081 cggctgaggc ggcgttgcct tcgacggctt ggacgcgcgc gtagcggcgg gcgagcggga
     7141 gcgtgaagag gccgacgccg cagtagaggt cgagggcgct cgcgccgttg gcgtcgccga
     7201 ggccggcgcg gacgagcggc gcgaggagcg cgtggttgat ctggaagaag cagccggcgc
     7261 cgaagcggta gcgctcgtgg ccgatggcgc gctcctgctc gcgcgcgtcc tcttcgccga
     7321 gcgggggcgt gagcgagacg ccgcggtcgc cagcgacggc ctcgaactcg gcggccccgt
     7381 cgggcaggtc gtcttcgttc atgcggcggc gcagggcggt gagtttctcc tgcacgggcg
     7441 gggcggcgac ggggcattcg accacgtcga tgacgtgatg cgtgccgcgc tcgtagtagc
     7501 cgaggtgttt gcggagcggg tcgtgctgcc agcgggcgcg cgagcggtag tgccactcgg
     7561 cgggcgaggg cgtgatgggg atgtcggcgg gcggttcgag gcgcgcgatg cggcggaggc
     7621 agtcgcggat gatgccgact ttggcttcga gctgcgcggg gtagtcgagc tgttggaagt
     7681 cgcagccgcc gcagcgaccg aagtaggggc agggcggctc gacgcgcgcg ggcgagggcg
     7741 cgagcaactc tttgatcgag gcgaaggcga cggcgccgcg gacgcgcgcg acgcggacga
     7801 gggcgcggtc gccgggcgcg gcgagcgcga cgaagacggt ctgcccgccc gcgtgcgcca
     7861 gcccgacccc gcccggcagg atgcgctcga cctcgacctc gacttcgacg cccacgcgca
     7921 gcgaggcgcc gcccggcgcc gcacgcccgc gcggagactg cttccttcct ctcatggctt
     7981 aaaagccgtg aaccgtgaac cgtcaaccgt gatgagttaa aacagcatcc ctctcaacac
     8041 tcgtcattcg ttactcgtca ccgcttttca cttgtcacgg ttgacggttc acggttcacg
     8101 gcttttgacc gttcacggct ttggtcgttt caggagtacg gatactgcgg ctggccgggc
     8161 tggtactggt cggcgctcca ttgcggcggg ggcggggcgg cgtactcggc gtcggcgttt
     8221 tccatctcca gttcgaggcc gccgacgaag ccggcggcga cgttgtagat gagcgcgccg
     8281 atcacgccgg cgatgaagcc gacgaccccc gtgaagatcg ggaagacgac catgatgacc
     8341 agcccgacgg cgatgctccc cgcgccggcc gcgccggtgt cgcgccctcc gccggccatg
     8401 atggccccgc cgacgaccat gaaaatcagg ccgtagatga cgccgataat caaaccgaac
     8461 gcggcgttcg tcaccgccgt gattttggcg taggagaaaa ctccgacgcg cttgacttgc
     8521 attttcgcca tgaatcggaa ctccttcttc cctcagaggg ggtttgactg ggtcagaggt
     8581 agaaaaggct gaggcgaggg acgccgtact cttcgcgcag cgccttcgcc agcaggttgt
     8641 cgagcgtctg ctcgtaggtg gcgcgctcca tccgcgcgcg gtaaggttca agctgctcgg
     8701 cggcgtgcgc gcgcttcgcg gccagcttcg gcgccgggag cggcgcgcgc accgcgcggt
     8761 cgagcagcgc ttcgaggtcg gtcagggagt cttcgagcgc ctgcgcgtcg ggccgcgccg
     8821 cgcgggcgaa gtcggcctca agctccgcga gccgcgccgc cgcgcgcgcg agggcttcgc
     8881 acagctcgtc ctcgccgact tcgcgccgcc gcgactcgcc cgcgcgcgcg agcgcctcac
     8941 ggcactcggc cagatgggcc gcgatgaccg cgcgcgggaa cgggagaccc gcggcctcgt
     9001 ccggctcgtt cgtttcgacg tcgcccgccg ctcccgcgtc gtgggccgcg tcgccggtct
     9061 tcgcgttcgc gccgcgctgc ccttcgagcc attcggcgta ctgcgcctcg acctcttcct
     9121 gacagtagta cagagacttg acggttcgct tccggggttt cgactcgtac gagtcgaacg
     9181 cgctctcgac gccccgcagg acgacgtgca gcggcactcc catccgtttc caactgtcca
     9241 tcaaagccca gtccatcgga ctcagcagca ggtgtttgcc gcgccgccgg atgaaggcgt
     9301 cttcgatctc ggtgaagtag ttgaagtagt tcaagcggtc gcgcgcggcc ccctgccgtg
     9361 cggcgcgcgc gccctgtgtg tcgctcatgc gaggttcgga tgttcggagc agagtgtcgg
     9421 acaaactggg aagacccgcc ggcagaaatt tcaagccgac agcatgagcg ggcggcgcgg
     9481 attagtcaag gggaaatagt aggcagtagg cggtaggcgg taggcagttc aaagatactc
     9541 acaagtacga gtttaacgac cggactccgg aggcgctttg actgcctact gcctactcct
     9601 tcataggtag ctcagccacc agtcgcggcg tcgctgcttg acgcccgcga accagcggca
     9661 gagcgggtag agcaggacga ggccgaccgc ccagaagacg tagacgaccc agaggtcgaa
     9721 gccgttgggg ttcgggaagg gcttgtctgc gaagttggcg aactgccacg cgacggactg
     9781 gccggcgagg tagccgacga ggatggcgag cgcgtgcgcc gtcagccact gccagacgta
     9841 gaagaagagc ggcacgcgcc cgaaggtgat gagcgcgcgc gcgagggggc cgcgccgccc
     9901 cgcgcgctcg aagaacgcga gcgccatgat ggccggcccg agcgtcatca gcaggaagag
     9961 cagcgacggc ggatacttcg ccgtgttcag gaaggagagc agtgtaaaga gcgcgcccct
    10021 gctttgcacg tcccacggct gcgggtcgcc gtagacgttg aaggcgcgca gcagcacgaa
    10081 ggcggcgatc atcgacgcgc cgagcgcgag caggatgcgc cgccgcctct ccggcgtcag
    10141 ttggtagagc gcgccgaagg cgtagcccgc ggccatcacg ccgacccagg ggatgagcgg
    10201 gtagatgatg aacgtggacg tgccgccggg cagcggcagc ggcccctgct ggtgcagcac
    10261 cagccacacg gcctgccaga agctcggctc cggcgtcccc ggcccctgcc agaaaggcac
    10321 cctgatgccg tcaagcaaat tgtgcagcac gatcaacagc acgccgaacg cgccgacgac
    10381 tcgcagcggc agatgaacga gcgcggcgag cacgatcatc gagacgccga tgacccagat
    10441 gacctgcgcc actccgagtt cgcgatagtc gaagttgaag agcaccgccg cgcggacgac
    10501 cgtgaactca agcacgacga gccacagc
//
LOCUS       DBNGOH010000417         4086 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1348257, whole genome shotgun sequence.
ACCESSION   DBNGOH010000417 DBNGOH010000000
VERSION     DBNGOH010000417.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4086)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4086)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4086
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1348257"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1210)
                     /locus_tag="AC159P_08210"
     CDS             complement(<1..1210)
                     /locus_tag="AC159P_08210"
                     /EC_number="3.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HIR3695475.1"
                     /translation="MSTDPISRRDLFKLAGAGAVATGLHAAAPSHAATRSPSDDQADR
                     ARRMKWWHEAKFGMFIHWGLYSVLGRHEWVLEMEGIPVEEYEPLAKRFLPKPGFAREW
                     ARLAKRAGQKYMVMTTKHHEGFCLFDTKTTGFCAPKQAAARDLVREYVEAVRAEGLRV
                     GFYFSLMVWHHPDGARCATDEAARKRFVAYIHTQVSELMTNYGKIDVLWYDVPWPLDA
                     KGWESERMNETVFKLQPDIIVNNRNRLPGDFSTPEQRIEAAEAGRAWEACMTMNDSWG
                     YHRADDAWKTPKQVLRNLITCAHDGGNYLLNIGPKADGSVPLESVQILGAVGRWMDKN
                     NRAIHGADICQPRRSRFASFSRKGHTLFMHVHHWPGDTVALAGLMNKVKSASLHATGQ
                     PVKFRQDAYRV"
     gene            complement(1367..>4086)
                     /locus_tag="AC159P_08215"
     CDS             complement(1367..>4086)
                     /locus_tag="AC159P_08215"
                     /inference="COORDINATES: protein motif:HMM:NF051698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HIR3695476.1"
                     /translation="FVRDPSLAGNCSASDTTACFRDPSRATPANPLGLNIVPRARWNA
                     SGAALLSYFPLPNAVGARTSAGSVYNYVVQSPARVPKQSQVLTFDIKPTSDDSFRFKA
                     QWWTSDNEGTGTSGWPGNDNNRWGISSHYLYKDNGWTANWIHIFSPSVVNEFNFGMRH
                     DSEGFIPSDGEVERLQRSALNYTAPQLFPDNNRLGTIPRVTNWGGVRGPTNGVANINW
                     LDRWGEIGNDYIKPSFANNLSWNRGNHALKFGVYYERVLNGEAPGGQWSGVFNFAGND
                     SAYTAALGNTSYAYANALIGNFRNYQESTARPFTNLELTLLQWYAQDQWKVNRSFTLN
                     YGLRLGYHSQWLQRDGRASNFVPELYNRANAPLLFESACAVSFTPPATCPAASRRARN
                     PATGELIQLVGANANLVGAIVPNTGNLLNGISVATDADAIRGFKDVQPVDWEPRVGFA
                     WDIRGDGKTVLRAMGGVYHSPRTGGGTTGGNLVNNVPQNRTLSIDFGSIDSLASLTGT
                     ALTRPSGLNAVESDSHTPTSYNFSLGVQQDIGYQTVLEVSYVGSLSRHLGERRNINQV
                     PDGAKLGTNNISPVTGSRLGDDFLRPFRGYGDINMVMYSGTANYHSMQAQVNRRYSNN
                     FQYGLAYTYSKSFDYANDDSSDVNNGRPYKAFNYAPSDFDQTHIFTVNYIWDVPGLGR
                     RFDNGFVRAVFDNWQISGQTSYASGKPKNIGVTYNSGTVDITRGQPCPVQSTRSAHPT
                     NAALDRCTPFTDWTGGDINARPFMICDPMQNVGGADPTGTPYVINRDCFAPPTALGQI
                     GNMPRNAVRMPSTFNTDLAFFKNIRWGERMTIQLRWETYNLFNHTNFRDIDAGLTFGL
                     VAVNPSPTGAACSATNVCTAEYRQTNTRFGAPIAARAPRVQQASIRISF"
BASE COUNT          603 a         1202 c         1531 g          750 t
ORIGIN      
        1 ggacgcggta ggcgtcctgc cggaacttga cgggctgacc tgtggcgtgc aaggaagccg
       61 acttgacttt gttcatcagt ccggcgaggg cgacggtgtc gccgggccag tggtggacgt
      121 gcatgaagag cgtgtggccc ttgcggctga aggaggcgaa gcgcgagcgg cgcggctggc
      181 agatgtccgc gccgtgaatc gcgcggttgt ttttgtccat ccagcggccc acggcgccca
      241 agatttggac ggactcaagc gggacggagc cgtcggcctt cgggccgatg ttgaggagat
      301 agtttccgcc gtcgtgcgcg caggtgatga ggttgcgcaa gacctgcttg ggcgtcttcc
      361 acgcgtcgtc ggcgcggtgg tagccccaac tgtcgttcat cgtcatgcag gcttcccagg
      421 cgcggcccgc ctcggcggct tcgatgcgct gttcgggcgt ggagaagtcg ccgggcaggc
      481 ggttgcggtt gttgacgatg atgtcgggct gaagcttgaa gaccgtctcg ttcatgcgct
      541 cggactccca acccttcgcg tcgagcggcc acggcacgtc gtaccagagc acgtcgatct
      601 tgccgtagtt ggtcatcagc tcggagacct gcgtgtggat gtatgcgacg aagcgcttgc
      661 gcgccgcctc gtccgtggcg cagcgcgcgc cgtccgggtg gtgccagacc atcagagaaa
      721 aatagaagcc gacgcgcaag ccctcggcgc gcaccgcctc gacgtactca cggacgaggt
      781 cgcgggccgc cgcttgcttc ggcgcgcaga agccggtggt cttcgtgtcg aagaggcaga
      841 agccctcgtg gtgcttggtc gtcatgacca tgtacttctg tccggcgcgt tttgcaagcc
      901 gcgcccactc gcgcgcgaag ccgggcttgg ggaggaagcg tttggcgagc ggctcgtact
      961 cctcgacggg gatgccctcc atctccagca cccactcgtg gcgaccgagc acgctgtaga
     1021 ggccccagtg gatgaacatg ccgaacttcg cctcgtgcca ccacttcatg cggcgcgcgc
     1081 ggtcggcctg atcgtcggac ggcgaacggg tcgcggcgtg cgacggcgcg gcggcgtgga
     1141 ggccggtggc gacggcgccc gcgcccgcga gcttgaagag gtcgcgcctc gatatcgggt
     1201 cggtgctcat cttcggagtc ctccgctttt agcgagagtc cacgcccccg agtctgtaaa
     1261 gccagagcag attcaggtcg tggactcttc ggtgtcggga agttggtcga aacggcggcg
     1321 gctgccgcgc ggactcgtcc ggcgcagccg cccccggcca cgcgaactag aagctgatcc
     1381 tgatcgaagc ctgctgcacg cgcggcgcgc gcgcggcgat gggcgcgccg aagcgcgtgt
     1441 tggtctggcg gtactccgcc gtgcagacgt tggtcgccga gcaggccgcg cccgtcgggc
     1501 tggggttgac ggcgaccagc ccgaaggtca ggcccgcgtc gatgtcgcgg aagttcgtgt
     1561 ggttgaacag attgtacgtc tcccagcgaa gctggatggt catgcgctcg ccccagcgga
     1621 tgttcttgaa gaaggcgagg tcggtgttga aggtcgaggg catgcgcacg gcgttacgcg
     1681 gcatgttgcc gatctggccg agcgcggtgg gcggcgcgaa gcagtcacgg ttgatgacgt
     1741 agggcgtgcc tgtcgggtct gcgccgccga cgttctgcat cgggtcgcag atcatgaagg
     1801 ggcgcgcgtt gatgtcgccg cccgtccagt ctgtgaaggg cgtgcagcgg tcgagcgcgg
     1861 cgttggtcgg gtgcgccgag cgggtggact ggacgggaca gggctgccca cgggtgatgt
     1921 ccacggtgcc gctgttgtag gtcacgccga tgttcttcgg cttgccgctg gcgtaggagg
     1981 tctgccccga tatctgccag ttgtcgaaga cggcgcggac gaaaccgtta tcgaagcggc
     2041 ggcccagacc gggcacgtcc cagatgtagt tgacggtgaa gatgtgcgtc tggtcgaagt
     2101 ccgaaggcgc gtagttgaac gccttgtagg ggcgcccgtt gttcacgtcc gaagagtcgt
     2161 cgttggcgta gtcgaaggac ttcgagtagg tgtaggccag tccgtactgg aagttgttac
     2221 tgtagcggcg gttcacctgc gcctgcatcg agtggtagtt ggcggtgccg gagtacatca
     2281 ccatgttgat gtcgccgtag ccgcggaagg ggcgcaggaa atcgtcgccg aggcggctgc
     2341 ccgtgaccgg gctgatgttg ttcgtgccga gcttggcgcc gtcgggcacc tggttgatgt
     2401 tgcgccgctc gccgaggtgg cggctgagcg agccgacgta gctgacttcg aggacggtct
     2461 ggtagccgat gtcctgctgg acgccgagcg agaagttgta gctggtcggc gtgtgcgagt
     2521 cgctctcgac cgcgttcagc cccgaggggc gcgtgagcgc cgtgccggtc agggacgcca
     2581 agctatcgat gctgccgaag tcgatggaga gcgtgcggtt ctgcgggacg ttgttgacga
     2641 ggttgccgcc cgtcgtgccg ccgcccgtgc gcggcgagtg gtagacgccg cccatcgcgc
     2701 gcaacaccgt cttcccgtcg ccacggatgt cccacgcgaa cccgacgcgc ggctcccaat
     2761 cgacgggctg cacgtccttg aagccgcgga tggcgtccgc gtcggtggcg accgagatgc
     2821 cgttgaggag gttgcccgtg ttcggcacga tggcgccgac gaggttggcg ttggcgccga
     2881 cgagctggat cagctcgccg gtcgcggggt tgcgcgcgcg gcgcgaggcc gccgggcagg
     2941 tcgcgggcgg cgtgaaagag acggcgcacg cgctctcgaa gagcagcggc gcgttggcgc
     3001 ggttgtagag ttcggggacg aagttcgacg cgcgcccgtc gcgttgcagc cactgcgagt
     3061 ggtagccgag gcgcagaccg tagttgagtg tgaaactgcg attcaccttc cactgatcct
     3121 gcgcgtacca ctggagcagc gtcagctcaa ggttggtgaa ggggcgcgcg gtcgattcct
     3181 gatagttgcg gaagttgccg atgagcgcgt tggcgtaggc gtagctggtg ttgccgagcg
     3241 cggcggtgta ggcgctgtcg ttgcccgcga agttgaagac gccagaccac tgcccgcccg
     3301 gcgcctcgcc gttgagcacg cgctcgtagt agacgccgaa cttcaaggcg tggttgccgc
     3361 ggttccacga gaggttgttg gcgaaagagg gcttgatgta gtcgttgccg atctcgcccc
     3421 agcggtcgag ccagttgatg ttggcgacgc cgttggtcgg gccgcgcacg ccgccccagt
     3481 tggtcacgcg cggtatcgtg ccgagccggt tgttgtcggg gaagagctgc ggcgccgtgt
     3541 agttcaaggc gctgcgctgc aagcgctcga cctcgccgtc cgaagggatg aagccctcgg
     3601 agtcgtgccg catgccgaag ttgaactcgt tgacgacgga cgggctgaag atgtgaatcc
     3661 agttggccgt ccagccgttg tccttgtaga ggtagtgcga gctgatgccc cagcggttgt
     3721 tgtcgttgcc gggccagccc gaggtgcccg tgccctcgtt gtccgaagtc caccactgcg
     3781 ccttgaagcg gaagctgtcg tcgctcgtcg gcttgatgtc gaaggtcaac acctggctct
     3841 gcttcggcac gcgggcagga ctctggacga cgtagttgta gacgctgccc gccgacgtgc
     3901 gcgcgccgac ggcgttcggc agcgggaagt agctgaggag ggccgcgccg ctcgcgttcc
     3961 agcgcgcgcg cgggacgatg ttgaggccga gcgggttcgc gggcgtggcg cgcgaggggt
     4021 cgcggaagca ggcggtcgtg tcgctcgcgc tacagttgcc cgcgagcgag gggtcgcgga
     4081 cgaaga
//
LOCUS       DBNGOH010000418         6041 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_982191, whole genome shotgun sequence.
ACCESSION   DBNGOH010000418 DBNGOH010000000
VERSION     DBNGOH010000418.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6041)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6041)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6041
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_982191"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3140)
                     /locus_tag="AC159P_08220"
     CDS             complement(<1..3140)
                     /locus_tag="AC159P_08220"
                     /inference="COORDINATES: protein motif:HMM:NF013736.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HIR3695477.1"
                     /translation="MPVPDTAAAVIADPEELLDASVRRLEALTALAAAPASGRELEEI
                     FAGLAATVTRLTDYRTCLVTLFSGEPPYHTRVLSRSANIPAEYVARFESGAYPRAAIA
                     ALLDKGVRIEVGELGFAAYYPPSHYHFLDEAAPDRVKADVERRPPPDGARPWHAGDEL
                     FVPIRTHEGELLGLISLDDPRTGRAPDRASVLPAVAFARHVAQLVARRRDAERLAAQA
                     EREQVVNRITRAVRQSLDTDEVFRTAVGELGQQLGVDRCVLFMLDREAGVARNLAEYV
                     APGQMPAGREYSIPLIAGLVEGVRAHGHLVFEDVANDPAIRGVYENILSRLGKRSIMY
                     VGIRVGSEMPAAFAVSVARERRRWRDSDVALARAVADQTGIAIRQAELYQRAEAAAAR
                     QVLINRLSHAIRATLNLPEVMHTATHELGQALSASRAYIRPYDPARPDFSPAMYEYLA
                     PGCRGVGSYGSSYARPVGLFLKTRLKTLVVNDARHHDDPSPEMNEHLREVAERGQTRS
                     MLFCPLVVQGRFRGSLVIHQTDRVRRWERDEIALVESVAAQLATGVAQAELFEMVARA
                     KRTWEVTFDAMSDGIFIFSNERRLVRVNRAGAAMEEAAPRELIGRSCCDVLRAGRGEG
                     CVVERVAAEGRAVTLEYTPERLNQTLLVTAEPLVEAAGLVGTVCTVRDLSELRRIESL
                     ARERQSLLENVLESARETIAALDSEGRIMWCNSAATALGGYAMEEIIGRSVFEMVEES
                     EVAPMRENLRRVLAGEALTVEVLFRTKEGEVRSAISDYAPLVIEGRVTGVLTISRDIT
                     ERKRERERAAQADKLRALGQLASGVAHDFNNALAAILGRAQLLRRTSRDPEVARGLDI
                     IQTAAEDAAATVRRIRAFAQQSPPGEGLAPLELRELLRDAVEITRTRWENDARARGLS
                     YDVRLDCDGAAPTRGNASELREVFVNLIVNAIDAMPAGGRLAITCRRRSARLRLDFKD
                     TGDGMSEEVRARIFEPFYTTKGVTGTGLGLFVSYGIVERHGGHISAASQPGRGTTFTI
                     DLPHAEASRDEAPSQRT"
     gene            complement(3440..4573)
                     /locus_tag="AC159P_08225"
     CDS             complement(3440..4573)
                     /locus_tag="AC159P_08225"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HIR3695478.1"
                     /translation="MFLRFKMTRVFVLALAATFAVAGWAPVRARVGGAQGQQQQQQQP
                     KPKATPQDGDEAIDDDDVVRFETDLTNVLFTAVDRNKRFVTTIKKEDVRVLEDGVEQE
                     VFTFQRETDRPLSLAILVDTSASQERTLPEEKSAAQRFVDAVIRSRRDEVAVLSFTGD
                     ATLEQGLTGNASNVRRAIDRIEFVPPSGYIGGGTVINVPGTTPPISGDAQRRAGSTAI
                     WDAVWVTARDVLSETSDKTRRAIILLTDGVDTSSTKKMSEAIDSALKADAIIYSIGIG
                     DDFFSGVEEDSLRQLSERTGGRAYFPRDETDLRVAFSQIQEELRSQYLLAYSPKNKAK
                     DGTFRKVQIEVVNPELQKQKLRLTYRQGYFAQSAAAPTPRKKG"
     gene            complement(4683..5711)
                     /locus_tag="AC159P_08230"
     CDS             complement(4683..5711)
                     /locus_tag="AC159P_08230"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HIR3695479.1"
                     /translation="MRTAVYWILFVTAVSAFVGGAAETFAQPNPQPQTTPSPQPQQDT
                     RPDVTYIREVRVPVTVTDKKGNPVTGLKREDFQVFEDKQQQEIKGFTGEIVELQKLPV
                     YVGVLMDTSPSASGKLKFQQEAALNFMYTVTRSRKDRVAFLTFDNEVQLRQDFTTNLD
                     QLNKAVSNVKKPGTHTALYDAVWQFCDEKMRSVGVGGRRVIVIISDGDDTYSRARIED
                     AIQIAQKTDTIIFAVSTKGGFAYSAVPGVEGGTVKDTGDKYLERMAEETGGRAFFTGD
                     MLALERAFKKIGEQLRSQYIVTYAPTNKEFDGRERRIEVKLLSKEDGWRVSAKRGFKA
                     DKDIIHQP"
BASE COUNT          859 a         1957 c         2248 g          977 t
ORIGIN      
        1 gtgcgttgcg acggcgcctc gtcgcgcgag gcttcggcgt gcgggaggtc gatggtgaag
       61 gtcgtgccgc ggccgggctg cgaggcggcg gagatgtggc cgccgtggcg ctcgacgatg
      121 ccgtagctga cgaagaggcc gaggccggtg ccggtgacgc ccttcgtcgt gtagaacggc
      181 tcgaagatgc gcgcgcgcac ctcctcgctc atcccgtcgc cggtatcctt gaagtcgagc
      241 cgcaggcgcg cgctgcggcg gcggcaggtg atcgcgagcc ggcccccggc gggcatcgcg
      301 tcgatggcgt tgacgatgag gttgacgaag acctcgcgca gctccgaggc gttgccgcgc
      361 gtcggggccg cgccgtcgca gtcgagccgc acgtcgtagg agaggccgcg cgcgcgcgcg
      421 tcgttctccc agcgcgtgcg cgtgatctcg acggcgtcgc gcagcagctc gcgcagctcg
      481 agcggggcca gcccctcgcc cggcggcgac tgctgcgcga aggcgcggat gcggcggacg
      541 gtggcggcgg cgtcctcggc ggcggtctgg atgatgtcga ggccgcgcgc gacctcgggg
      601 tcgcgcgacg tgcgccgcag gagctgcgcg cgcccgagga tggcggcgag cgcgttgttg
      661 aagtcgtgcg cgacgcccga ggcgagctgg cccagagcgc gcagcttgtc ggcctgcgcg
      721 gcgcgctcgc gctcgcgctt ccgctcggtg atgtcgcgcg agatggtgag gacgcccgtc
      781 acgcggccct cgatgacgag cggcgcgtag tccgagatgg cagagcgcac ctcgccctcc
      841 ttcgtgcgga acaacacctc gaccgtcagc gcctcgcccg cgagcacgcg gcgcaggttc
      901 tcgcgcatcg gcgcgacctc gctctcctcg accatctcga agacgctgcg accgatgatc
      961 tcctccatcg cgtaaccgcc gagcgccgtc gccgccgagt tgcaccacat gatgcggccc
     1021 tcggagtcga gggcggcgat ggtctcgcgc gcgctctcca acacgttctc caacaaagac
     1081 tgccgctcgc gcgcgagcga ttcgatgcgg cgcagctccg acaggtcgcg caccgtgcag
     1141 accgtgccga cgaggcccgc cgcttcgacg agcggctcgg cggtgacgag cagcgtctgg
     1201 ttcaggcgct cgggcgtgta ttcgagcgtg acggcgcgcc cttcggcggc gacgcgctcg
     1261 acgacgcagc cctcgccgcg gcccgcgcgc agcacgtcgc agcagctccg cccgatcagc
     1321 tcgcgcggcg cggcctcctc catcgccgcg cccgcgcggt tgacgcgcac gagccgccgc
     1381 tcgttcgaga agatgaagat gccgtccgac atcgcgtcga acgtgacctc ccacgtgcgc
     1441 ttggcgcgcg cgaccatctc gaacagctcg gcctgcgcca ccccggtggc gagctgcgcg
     1501 gcgaccgact cgacgagcgc gatctcgtcg cgctcccagc ggcggacgcg gtcggtctgg
     1561 tggatgacga gcgagccgcg gaagcgcccc tgcacgacga gcgggcagaa gagcatcgag
     1621 cgcgtctggc cgcgctcggc cacctcgcgc aggtgctcgt tcatctcggg cgaggggtcg
     1681 tcgtggtggc gggcgtcgtt gacgacgagc gtcttgagcc gcgtcttcag gaagaggccg
     1741 accgggcgcg cgtaggagct gccgtacgag ccgacgccgc ggcagccggg cgcgaggtac
     1801 tcgtacatcg cgggcgagaa gtccgggcgc gcggggtcgt acgggcggat gtaggcgcgc
     1861 gaggccgaga gcgcctgacc cagctcgtgc gtcgccgtgt gcatcacctc gggcaggttg
     1921 agggtggcgc ggatggcgtg gctcaggcgg ttgatgagca cctggcgcgc ggcggcggcc
     1981 tcggcgcgct ggtacagctc ggcctgccgg atggcgatgc cggtctggtc tgcgacggcg
     2041 cgcgcgagcg ccacgtccga gtcgcgccag cggcgcctct cgcgcgcgac cgagacggcg
     2101 aaggcggcgg gcatctccga gccgacgcgg atgccgacgt acatgatcga gcgcttgccg
     2161 agtcggctga ggatgttctc gtagacgccg cggatggccg ggtcgttcgc cacgtcctcg
     2221 aagacgaggt ggccgtgcgc gcggacgcct tcgacgaggc cggcgatgag agggatgctg
     2281 tactcgcgtc ccgcgggcat ctggccgggc gcgacgtact cggcgaggtt gcgcgcgacg
     2341 ccggcctcgc ggtcgagcat gaagagcacg cagcggtcaa cgccgagctg ttggcccagc
     2401 tcgccgaccg cggtgcggaa gacctcgtcc gtgtcgagcg actggcgcac ggcgcgcgtg
     2461 atgcggttga ccacctgctc gcgctcggcc tgcgcggcga gccgttcggc gtcgcggcgg
     2521 cgcgcgacga gctgcgcgac atggcgcgcg aaggcgaccg cgggcagcac gctcgcgcgg
     2581 tcgggcgcgc ggcccgtgcg cgggtcgtcg agcgagatga ggccgagcag ctcgccctcg
     2641 tgcgtgcgga tcggcacgaa cagctcgtcg cccgcgtgcc acggtcgcgc gccgtccggc
     2701 ggcgggcgcc gctccacatc ggccttgacg cggtcgggcg cggcctcgtc cagaaagtgg
     2761 tagtggctcg gcgggtagta ggccgcgaag cccagctcgc cgacctcgat gcgcacgccc
     2821 ttgtcgagca gcgcggcgat ggccgcgcgc gggtacgcgc ccgactcgaa gcgtgcgacg
     2881 tactcggcgg ggatgttggc cgagcgggag agcacgcgcg tgtggtaggg cggctcgccg
     2941 gagaagaggg tgacgaggca ggtgcggtag tcggtgagcc gcgtgacggt cgcggccagc
     3001 cccgcgaaaa tctcttccag ctcgcgcccc gaggcgggcg cggcggcgag cgccgtcaac
     3061 gcttcgaggc gacgcacgga ggcgtcgagc aactcctcgg ggtccgcgat cacggcggcg
     3121 gcggtgtcag gaacaggcat ggtgtcgggg cggcggggcc gggcagtccc gcgaaaaaca
     3181 atccgcggag tcgggcgtga gtccgcgcgt gcgaagggtt gccagattga ggcgcttccg
     3241 gctctcggga gtgacgtggg gcgctcgtgc ctccttcaag cctgacggcc cgacgggtgg
     3301 aggtcgcggg ccgcacgacg gagtataaac gaagaggggg aaaagggtaa gaggaaagcg
     3361 ggaaggggga aaagggtaaa agggtaaaag gcgaaaaggg agagagcggc gcacgtttcc
     3421 tgctctccct tttcgccttt taccccttct tccggggcgt gggcgcggcg gcgctctgtg
     3481 cgaagtagcc ctggcggtag gtgagccgga gtttctgttt ttgcagctcg gggttgacga
     3541 cttcgatctg cactttgcgg aaggtgccgt ccttcgcctt gttcttgggc gagtaggcca
     3601 gcaggtattg cgagcgcagc tcttcctgaa tctggctgaa ggcgacgcgc aggtcggtct
     3661 cgtcgcgcgg gaagtaggcg cggcccccgg tgcgctcgga gagctggcgt agagagtctt
     3721 cctcgacgcc gctgaagaag tcgtcgccga tgccgatgga gtagatgatg gcgtcggcct
     3781 tgagcgcgga gtcgatggcc tcgctcatct tcttggtgct gctcgtgtcc acgccgtcgg
     3841 tcagcaggat gatggcgcgg cgcgtcttgt cggaggtctc ggagagcacg tcgcgggccg
     3901 tgacccagac ggcgtcccag atggcggtcg agccggcgcg gcgctgggcg tcgccggaga
     3961 tgggcggcgt cgtgccgggg acgttgatga cggtgccgcc gccgatgtag cccgagggcg
     4021 ggacgaactc gatgcggtcg atggcgcggc gcacgttcga ggcgttgccc gtgaggccct
     4081 gttcgagcgt ggcgtcgccg gtgaaggaga gcacggccac ctcgtcgcgg cgcgagcgga
     4141 tgacggcatc gacgaaccgc tgcgcggcgc tcttctcctc gggcaaggtg cgctcctgcg
     4201 aggcgctcgt gtccacgagg atggcgagcg agagcgggcg gtcggtctcg cgctggaagg
     4261 tgaagacctc ctgctcgacg ccgtcttcga ggacgcgcac gtcctccttc ttgatcgtcg
     4321 tcacgaagcg cttgttccta tcgaccgccg tgaagagcac gttcgtcagg tcggtctcga
     4381 agcgcaccac gtcgtcgtcg tcgatcgcct cgtcgccgtc ctgcggcgtg gccttcggct
     4441 tcggctgctg ctgctgttgc tgctgcccct gcgcgccccc cacgcgcgcg cgcacgggcg
     4501 cccagcccgc gaccgcgaag gtcgccgcca gcgccagcac gaacacgcgc gtcatcttga
     4561 atcgcaaaaa cataaaagcc gtgaaccgta aaccgtgacg agtgaaaagc ggtgagccgt
     4621 gaatcgtaaa ccgtgataag tcgaaaccct gttcttactt gtcacggttc acggcttacg
     4681 gttcacggct gatgaatgat gtccttgtcc gccttgaagc cgcgcttggc gctgacgcgc
     4741 cagccgtcct ccttgctgag gagcttgact tcgatgcggc gctccctgcc gtcgaactcc
     4801 ttgttcgtcg gcgcgtaggt gacgatgtac tgcgagcgca actgctcgcc gatcttcttg
     4861 aaggcgcgtt cgagcgccag catgtcgccg gtgaagaagg cgcgcccgcc cgtctcctcg
     4921 gccatccgtt cgaggtactt gtcgccggtg tccttgacgg tgccgccctc gacgccgggc
     4981 acggcgctgt aggcgaagcc gcccttcgtc gagacggcga agatgatggt gtcggtcttc
     5041 tgcgcgatct ggatggcgtc ctcgatgcgg gcgcggctgt aggtgtcgtc gccgtcgctg
     5101 atgatgacga tgacgcgccg cccgccgacg ccgaccgagc gcatcttctc gtcgcagaac
     5161 tgccacacgg cgtcgtagag cgcggtgtgc gtgcccggct tcttgacgtt gctgacggcc
     5221 ttgttcagct ggtcgaggtt ggtcgtgaag tcctgccgga gctgcacctc gttgtcgaag
     5281 gtgaggaacg cgacgcggtc tttgcgcgag cgcgtcacgg tgtacatgaa gttgagcgcc
     5341 gcctcctgct ggaacttcag cttgcccgag gccgacggcg aggtgtccat cagcacgccg
     5401 acgtagacgg gcagcttctg caactccacg atctcgccgg tgaagccctt gatctcctgc
     5461 tgctgcttgt cctcgaagac ctgaaagtct tcccgcttca gccccgtcac cgggttgccc
     5521 ttcttgtccg tcacggtgac gggaacgcgc acctcgcgga tgtaggtcac gtcgggccgc
     5581 gtgtcctgct gcggctgcgg cgatggtgtg gtctgtggct gcggattcgg ctgcgcgaag
     5641 gtctcggcgg cgccgccgac gaaggcggaa actgcggtga cgaataaaat ccagtaaacg
     5701 gcggttctca tagtctcgtt tgcaaaggag cgcggggtta gagaaacgtt cttccttgcg
     5761 acgccgccgt cccgcttcca aaggcgctgt ttcgatgcca cccgggagga cggtcgttgc
     5821 ttcgaatttg gacgccagta tatcaaccaa cccgccgccc aagccacctg cggccccacc
     5881 gcacggcgcc gccaagccgc tcgggacgcg cgctcattca agctcgtcga ggaaggtctg
     5941 tagctcgacg gggagcgggg cggcgaagcg caggcgttcg ccggtgcgcg ggtgtgtgaa
     6001 ggcgagttgt tcggcgtgga ggaactggcg acccagggcg g
//
LOCUS       DBNGOH010000419         6054 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3730624, whole genome shotgun sequence.
ACCESSION   DBNGOH010000419 DBNGOH010000000
VERSION     DBNGOH010000419.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6054)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6054)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6054
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3730624"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1495
                     /locus_tag="AC159P_08235"
     CDS             <1..1495
                     /locus_tag="AC159P_08235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR3695480.1"
                     /translation="ATFTAVEIGPSTELVYRFNLQPAGEGRTTAERRPDRDDPKFKLR
                     SQHARRSIFHGVEGEDETVEAAVAAIEADEDEEAAAALDEEGFAVGDGNDDERRARTR
                     PQGFVETYFASSANPRVGNYAGVNFALAAPVSREFEVIFSGQLGEFERLEATARFRPN
                     ARHRVSATVGGARLPLTPKGTEDFGADKLGQFSVRAVDEWVVREGVVVVLGLDYSRFV
                     GAGGADSFSPRVGFAFDADARTRFRAAYAPGSDAAESIEGINFEEGAILFKELPGQAL
                     ALVDGQAVMDRSSRLEFGVERVLSENSSVEATAFFDTVDNRGVGLFSAPLDALSDAGS
                     AALIDIANQQGGARGLRVVYTRRVTEHLRASAGYAFGRGQQLSAEGFAGGPEQIFRGA
                     FFQTAAAQLDADLLDGTNVRTVLRFSPRAAVFAIDPFAGRLAVYDPSLSILVTQELPT
                     FGLPLRARATIDARNLLDVMTGIEDGENTVSLNVLRRTVRGGISVRF"
     gene            1892..4882
                     /locus_tag="AC159P_08240"
     CDS             1892..4882
                     /locus_tag="AC159P_08240"
                     /inference="COORDINATES: protein motif:HMM:NF024883.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR3695481.1"
                     /translation="MKGSVLTTKAWALLILAALFAAAGTLNFAQRLNKTPPPTDGVEW
                     VEAGPKNFVARSVDPDSPAGTNRKGVFGIMPGDRLIAISLDEKTDEEIGRLSDIQADL
                     EMAGVGGRIRYLIERPTNPEEIRFYHLHLDNLTPVPTWGSRDLYINLVGIIYLLVGLF
                     VLFRQGATAPFVQHFMVLCLAAFVFHFYKPYGSYEDFDLFISFLDETALTVFAPLFLH
                     FCALYPVRYRLSERRPWLVYLLYVPALVLVALDALFLLPVVSPRLSFLSGFFPDDFPA
                     RLWLAEVTCFTIALAVGASLLVRRFWKSESAIVRQQLKWVVWGSALAVLPFTLFYAAG
                     YAFGGALDIYVSETAKRWLTGAAIVPLALIPLTFGNSVVRYRLMDVDMVVRRTAVYAL
                     TTLAIALMLGTVVYVAGLYAFGGAVITPGVISMRVIIAVVAMAVIVMTAAPLKNFLQE
                     QTDRFFYGESYDLRHGLLDFGRTLSATTSLDPLLDALVGRLRQVLGVERIAIFIEDAR
                     APAGYRVARAAGLSSEVIVPEDFREMIRTRSAEDGIVRADGIDLPPESSGFVRRTLHY
                     YVPCVVRDRMVAVIGLGRSTGGALLSSEDLEILRTVSGYVAVAIENSLLYQEQRARAA
                     ELELLKEFNESIVESINVGLLAVDTEGRVTACNTALEELLGVSRGEAVGRKVEDLFAE
                     EFADTLAQVLGAAGWELSELRHIYKLHTATRASRPLILNIAVAPLKRADSPDHTGALV
                     VLEDVTGRVRLEEQLQQREKLSSIGLLAAGVAHEINTPLTGVSSYTQMLLGMLADTDP
                     KHALLQKISRQADRATGIVNNLLNFSRTGGLTDFTEVNVNRVLEDTLQLLEPQLRHGQ
                     IEIAREFSRELPSVFGNAGKLQQVFTNLLLNARDAIPAGGRVTLRTSAENDGGPDGAV
                     TVEVIDTGIGIAPENVARIYDPFFTTKGVGRGTGLGLAVSYGIVQEHSGHIAVESAPG
                     RGTTFRITLPVAEARAARLQAVGD"
     gene            5080..>6054
                     /locus_tag="AC159P_08245"
     CDS             5080..>6054
                     /locus_tag="AC159P_08245"
                     /inference="COORDINATES: protein motif:HMM:TIGR02276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HIR3695482.1"
                     /translation="MLTFPRRRNALVRTLALLFTPLAAAAWLMQSPAVPRAAAQVTPP
                     AFRNFEGPQVHPLALTPDGTRLLAVNTPNATLSVFQLTGEAPLLTAEIPVGLEPVSVA
                     ARNNREAWVVNWLSDSVSIVDLTTGNVTRTIEVGDEPTDVLFAGVAREMAFVCVSGGG
                     RVNVSSGLPITGGSGVVKVFDPASPAAAPQTLNVFGKQPRALARDAAGARVFVSVVES
                     GNKTTIVPESIVRANGGMPPPNPAMSGSLPPAPGTGLIVRWDGANWVDEIGRSWNQHV
                     PYRLADVDVAVFNSAGTTAAPAGDVRGVGTHVGNMAFDPATSRLFVANL"
BASE COUNT          882 a         2251 c         1935 g          986 t
ORIGIN      
        1 ggccaccttc acggcggtcg agatcggccc ctccacggaa ctggtttacc gcttcaacct
       61 tcagcccgcg ggcgaagggc gcaccaccgc cgagcgccgc cccgaccgcg acgaccccaa
      121 attcaaactc cgctcgcagc acgcgcgccg ctccatcttc cacggcgtcg agggcgagga
      181 cgagacggtc gaagccgccg tcgccgcgat tgaagcagac gaggacgaag aggccgccgc
      241 ggcgctcgac gaagagggct tcgcagtcgg cgacggcaac gacgacgagc gccgcgcgcg
      301 cacccgcccg cagggcttcg tcgagaccta cttcgcctct tccgccaacc cgcgcgtggg
      361 caactacgcg ggcgtcaact tcgcgctcgc cgccccggtc agccgcgagt tcgaagtgat
      421 cttctcgggg cagctcggcg agttcgagcg cctcgaagcg accgcgcgct tccggcccaa
      481 cgcgcgccac cgcgtgagcg ccacggtcgg cggcgcgcgc ctgccgctca cgccgaaagg
      541 caccgaagat ttcggcgcgg acaagctcgg gcagttttcc gtgcgcgccg tggacgagtg
      601 ggtcgtgcgc gaaggcgtgg tggtcgtgct cgggctggac tactcgcgct tcgtcggcgc
      661 gggcggcgcc gactccttca gcccgcgcgt cggcttcgcc ttcgacgcgg acgcgcgcac
      721 gcgctttcgc gcggcctacg cgcccggctc ggacgcggcg gaatcgatcg agggcatcaa
      781 tttcgaggag ggcgccatcc tcttcaagga gctgcccggt caggcgctcg cgctcgtgga
      841 cgggcaggcc gtgatggatc gcagcagccg cctggagttc ggcgtcgagc gcgtgctctc
      901 ggagaattcg agcgtcgagg ccacggcctt cttcgacacg gtcgataatc gcggcgtcgg
      961 cctcttcagc gcgccgctcg acgcgctctc ggacgcgggc agcgccgccc tcatcgacat
     1021 cgccaaccag cagggcgggg cgcgcggcct gcgcgtcgtc tacacgcgcc gcgtcaccga
     1081 gcacctcagg gcttccgccg gctacgcctt cgggcgcggg cagcagcttt ccgccgaggg
     1141 ctttgcgggc ggccccgagc aaatcttccg cggcgccttc ttccagaccg ccgccgccca
     1201 gctcgacgca gacctcctcg acggcaccaa cgtccgcacc gtgctccgct tctcgccgcg
     1261 cgccgccgtc ttcgccatcg accccttcgc cggtcggctc gcggtctacg acccgagctt
     1321 gagcatcctc gtcacgcagg agctgcccac cttcgggctg ccgctgcgcg cgcgcgcgac
     1381 catcgacgcg cgcaacctgc tcgacgtgat gacgggcatc gaggacggcg agaacacggt
     1441 ctccctgaat gtcctgcgcc gcacggtgcg cggcggcatc tccgtcagat tttgagtggg
     1501 gcgggcacct gctgcccgtc ccctatagcc ttccgcatcc ccgcgtcgcc gcccgaaaac
     1561 ggcaaaggcg cggggttttt ccatctccgg gcgcatacga attttcgtag gtttttacga
     1621 aaaactggat aatgtggccg cgatgtatac aggcggtgca cgggcgcccg cgacgtaaac
     1681 agcggggagc gcaagcgatg ccgcaagagg gcggcgcccg cgcgcggcgc aaaacgcagg
     1741 tgtcgggact ggaatagtgc ttgcttctgc attttagatt tcagcgaggc ttcactcacg
     1801 gggccgtctg gcggcggaca agagcgcggg cggcttccgc gtatccgaag tttcagagat
     1861 tcgtatccga agtttcagag aagagcgtgc gatgaaaggt tccgtgctga cgacgaaggc
     1921 gtgggcgctg ctgatactgg cggcgctgtt cgccgctgcc ggcacgctca acttcgcgca
     1981 gcgcctcaac aagacgccgc cgcccacgga cggcgtcgag tgggtcgagg ccggccccaa
     2041 aaacttcgtc gcgcgctcgg tggacccgga ctcgcccgcc ggcaccaacc gcaagggcgt
     2101 cttcggcatc atgcccggcg accgcctgat cgccatcagc ctcgacgaga agacggacga
     2161 ggagatcggg cgcctctccg acatccaggc cgacctggag atggccggcg tcggcgggcg
     2221 catccgctac ctgatcgagc gcccgacgaa ccccgaggag atacgcttct accacctcca
     2281 cctcgacaac ctcacgcccg tgccgacgtg gggttcgcgc gacctctaca tcaacctcgt
     2341 cggcatcatc tacctgctgg tcggcctctt cgtgctcttc cggcaggggg cgacggcgcc
     2401 cttcgtccag cacttcatgg tgctctgtct ggcggccttc gtcttccact tctacaagcc
     2461 ctacggctcc tacgaagatt tcgacctctt catctcgttc ctcgacgaga cggcgctgac
     2521 cgtcttcgcg ccgctcttcc tgcacttctg cgcgctctac cccgtccgct accgcctctc
     2581 cgagcgcagg ccgtggctcg tctacctgct ctacgtcccg gcgctcgtgc tcgtcgcgct
     2641 cgacgcgctc ttcctgctgc cggtcgtctc gccgcggctc tcgttcctct ccggcttctt
     2701 ccccgacgac ttccccgcgc ggctctggct ggccgaggtc acgtgcttca ccatcgcgct
     2761 cgcggtcggc gcaagcctgc tcgtgcgccg cttctggaag agcgagtcgg ccatcgtgcg
     2821 gcagcagttg aagtgggtgg tgtgggggtc ggcgctggcg gtgctgccgt tcacgctctt
     2881 ctacgcggcg ggctatgcct tcggcggcgc gctcgacatc tacgtcagcg agacggccaa
     2941 gcgctggctg acgggcgcgg ccatcgtgcc gctcgcgctc atcccgctca cgttcggcaa
     3001 ctcggtcgtg cgctaccgcc tgatggacgt ggacatggtc gtgcgccgca ccgccgtcta
     3061 cgcgctcacc acgctcgcca tcgccctcat gctcggcacg gtcgtctacg tcgcgggcct
     3121 ctacgccttc ggcggcgccg tcatcacgcc gggcgtcatc tcgatgcgcg tcatcatcgc
     3181 ggtggtcgcg atggccgtca tcgtgatgac cgccgcgccg ctgaagaatt tcctgcaaga
     3241 gcagaccgac cgcttcttct acggcgagag ttacgacctg cgccacgggc tgctggactt
     3301 cgggcgcacg ctctcggcga cgacctcgct cgacccgctt ttggacgcgc tcgtcggtcg
     3361 gctccggcag gtgctcggcg tcgagcgcat cgccatcttc atcgaagacg cgcgcgcccc
     3421 ggcgggctac cgcgtcgcgc gcgccgccgg gctgtcgagc gaggtgatcg tccccgaaga
     3481 cttccgcgag atgatccgca cgcgctcggc ggaggacggt atcgtccgcg ccgacggcat
     3541 cgacctgccg cccgaatcct cgggcttcgt gcgccgcacg ctccactact acgtgccctg
     3601 cgtcgtgcgc gaccgcatgg tcgccgtcat cggcctcggg cgctcgacgg gcggcgcgct
     3661 cctctcttcg gaagacctcg aaatcctccg caccgtctcg ggctacgtcg cggtcgccat
     3721 cgagaacagc ctgctctacc aggagcagcg cgcgcgcgcc gccgagctgg agctgctgaa
     3781 ggagttcaac gagtccatcg tcgagtcgat caacgtcggc ctgctggccg tggacacgga
     3841 ggggcgcgtg acggcctgca acacggcgct cgaagagctg ctcggcgtct cgcgcggcga
     3901 ggccgtgggc cgcaaggtcg aagacctctt cgccgaggag ttcgccgaca cgctcgcgca
     3961 ggtcttgggc gccgccggct gggaactctc cgagctgcgc cacatctaca agctccacac
     4021 ggcgacgcgc gccagccgcc cgctcatcct caacatcgcg gtcgcgccct tgaagcgcgc
     4081 cgactcgccc gaccacaccg gcgcgctcgt cgtcctcgaa gacgtgacgg ggcgcgtgcg
     4141 gctcgaagag cagttgcagc agcgcgagaa gctctcctcc atcggccttt tggcggcggg
     4201 cgtcgcgcac gagatcaaca cgccgctgac gggcgtttcg tcctacacgc agatgctcct
     4261 cggcatgctc gccgacaccg accccaagca cgcgctcttg cagaagatca gccggcaggc
     4321 cgaccgcgcc accggcatcg tcaacaacct gctcaacttc tcgcgcacgg gcggcctcac
     4381 ggacttcacc gaggtcaacg tcaaccgcgt gctcgaagac acgctccaac tgcttgagcc
     4441 gcagctccgg cacggtcaga tcgagatcgc gcgcgagttc tcgcgcgaac tgccgtccgt
     4501 cttcggcaac gcgggcaagc tccagcaggt cttcacgaac ctgctgctga acgcgcgcga
     4561 cgccatcccc gcgggcggtc gcgtcacgct ccgcacctct gccgagaacg acggcggccc
     4621 cgacggcgcc gtcaccgtcg aagtcatcga caccggcatc ggcatcgcgc ccgagaacgt
     4681 cgcgcgcatc tacgacccgt tcttcacgac caagggcgtc gggcgcggca ccggcctcgg
     4741 cctcgccgtc tcctacggca tcgtgcagga gcactccggc cacatcgccg tcgagagcgc
     4801 gcccggtcgc ggcaccacct tccgcatcac gctccccgtc gcggaggcgc gggccgcgcg
     4861 cctccaagcc gtcggcgact gaactttggt aaatagtgaa tagtaaatgg tgagtagagg
     4921 gaaagccgtc tctattcacc atttactatt caccgcgttt tctgttgaca cgggcgcgct
     4981 gcgagccgta ctcttcgcgc atatttgcct gacgccgggc cgtcttcccc ccggcagggc
     5041 tttcccgatt accgtccgtc ggctccacgg agtaccccca tgctcacctt cccccgtcgc
     5101 cgcaatgctc tcgttcgcac gctcgcgctt ctcttcacgc cgctcgccgc cgccgcgtgg
     5161 ctcatgcaaa gtcccgcggt gccgcgcgcc gcggcgcagg tcacgccgcc cgcgttcagg
     5221 aatttcgagg ggccgcaggt gcacccgctc gcgctgacgc cggacggcac gcgcctgctc
     5281 gcggtcaaca cgccgaacgc gacgctctcg gtcttccagt tgaccgggga ggcgccgctc
     5341 ctgaccgcgg agattcccgt cgggctcgag ccggtgagcg tcgccgcgcg caacaaccgc
     5401 gaggcgtggg tcgtcaactg gctctcggac tcggtgagca tcgtcgatct cacgaccggc
     5461 aacgtcacgc gcaccatcga ggtcggcgac gagccgaccg acgtgctctt cgcgggcgtc
     5521 gcgcgcgaga tggccttcgt ctgcgtctcg ggcggcgggc gcgtcaacgt cagctcgggc
     5581 ctgccgatca cgggcgggag cggcgtcgtc aaggtcttcg acccggccag ccccgccgcc
     5641 gcgccgcaaa ccctcaacgt cttcggcaaa cagccccgcg cgctcgcgcg cgacgccgcg
     5701 ggcgctcgcg tcttcgtctc cgtcgtcgag tcgggcaaca agacgaccat cgtccccgag
     5761 tcgatcgtgc gcgccaacgg cgggatgccg ccgcccaatc cggccatgtc cgggagcctg
     5821 ccgcccgcgc ccggcaccgg cctcatcgtc cgctgggacg gcgcgaactg ggtggacgag
     5881 atcggcaggt cttggaacca gcacgtgcct taccgcctgg ccgacgttga cgtggcggtc
     5941 ttcaactcgg cgggcacgac cgccgcgccc gcgggcgacg tgcgcggcgt cggcacgcac
     6001 gtcggcaaca tggccttcga cccggcaacg agccgcctct tcgtcgccaa cctc
//
LOCUS       DBNGOH010000420         9473 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1898330, whole genome shotgun sequence.
ACCESSION   DBNGOH010000420 DBNGOH010000000
VERSION     DBNGOH010000420.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 9473)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9473)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9473
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1898330"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1359)
                     /locus_tag="AC159P_08250"
     CDS             complement(<1..1359)
                     /locus_tag="AC159P_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007080578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HIR3695483.1"
                     /translation="MLRQCTALVLLCGALAAGLAPSAAAQTTVPPINFKERALANGMK
                     VYTVQDKSSPTVSIQVWYKVGSKDDPEGRSGFAHLFEHLMFKSTKNMKDEMMDRLTED
                     VGGWNNASTWDDFTNYYEVVPSNYLETLLWAEADRMGSLNVNDKNFPSERDVVKEEFR
                     SRILAPPYGRFLYSIEKNSYTTHPYRRPGIGSIENLDAATLEDVRAFHKTFYRPDNAT
                     LVVVGDFEQKQLDTWIDKYFGRVPKPGTPLPRVTAKEPARTAEKRFDETGPNVPLPAV
                     AITYLAPPVVSDDAYAIRVAESILSAGESSRLYQSLVYKQQVAQAAWVTADLREDNGL
                     VYFASILASGKQPAEAERALLAELKLMQDTPVSKAELDKAVNQLVTNQLRERETNNGK
                     ADAIGRAAVLMGDASRVNSDISKLQAVTAADVQRVMKKYFNDTNRVVITYTGEPAKPQ
                     ASD"
     gene            complement(1773..2174)
                     /locus_tag="AC159P_08255"
     CDS             complement(1773..2174)
                     /locus_tag="AC159P_08255"
                     /inference="COORDINATES: protein motif:HMM:NF013426.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HIR3695484.1"
                     /translation="MEEVSTTDERERLCALAPVTAAPIRHHVLVCNGTSCTAVGSAAV
                     KEAFDRELTARGLLMGKAAKGKNPLGSVVLTDCSSVGFCAVGAAVLVYPEGVWYARVH
                     ADDVAEIIEEHIVGGRVVERLALFRVPATKE"
     gene            complement(2174..2560)
                     /locus_tag="AC159P_08260"
     CDS             complement(2174..2560)
                     /locus_tag="AC159P_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003789471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR3695485.1"
                     /translation="MMERLEQNKKTVTAFYDLMFNQCRPAEAIERYTGDTYIQHNPVV
                     ADGKEAFIEYFTRMASEYPQKRVEFKRVIAEGNYVVLHCYQHWPGDGEWAGIDIFRLD
                     TDGKVVEHWDVLQRVPESSANDNTMF"
     gene            2783..4420
                     /locus_tag="AC159P_08265"
     CDS             2783..4420
                     /locus_tag="AC159P_08265"
                     /inference="COORDINATES: protein motif:HMM:NF024758.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HIR3695486.1"
                     /translation="MANGLVIHIARGQDRHTEVLSLERVRIGPGEDCHIRLAPDAPTD
                     STLLLELARTNGNYRVTEFDPASGPTINGVPIEHGDAIEDGDELRFAGAADLAVQFFP
                     VRELPAVVAHRRAQVAPFIEQAAIEAAVTARRDDAKVFLREFTRELLREINLTTKIGV
                     VLVTVILVGGLLYLGNAARRELKRSRDLIDKQNEQLTRMGEALDGTRQKFDDVDKKSQ
                     DIIASLSLAPRLRGEYGNGVCLIAGSYQLVEAGTGRPLRYPETQQNEDGSVISNGAEQ
                     PVLTPEGNGAPYIADFVGTGFHVGGGYIVTNRHLVVEPWTANDGVQSLSASVSGQFRV
                     TRLVAFFPDHTQPYVLRVRQATARDDLAVGQIDPAQMPADVPALPLDKDSGAAGIGKA
                     VVMMGYPSGEERLLATLPEGESRSIMQRYGSSIETLLAHLAERNYIKPLTTQGHITDV
                     DARRLEFDARNAVGGSGSPVFGQSGGVIGVNFAAFTGMPDANYAVPARFVYPLLERSG
                     WKSPEPDDKDTDANANTSNTKDAKDSRVATTQTNQSR"
     gene            4724..6439
                     /locus_tag="AC159P_08270"
     CDS             4724..6439
                     /locus_tag="AC159P_08270"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HIR3695487.1"
                     /translation="MSASAATVTTASIRPELFPGQIARPSSRAMVVALLAFLVVGGFG
                     LRAYRLSAEALSEDELNKVRAVADYRTRGLTPANGEHPFLMKALLAASTVACEAWNAT
                     SIADSNPQLRVSTETAVRLPGALLGALTALLIFLVASELFGFEVGLFAAALWAFDPAA
                     IGFNRIAKEDSFVVFFFLLANVFWLRGQRVAEGGHARPEPYYWAAAAAFGAMMASKYV
                     PFYIAISGAYYYAFQGVKATRWRMGKKKWLVFFAVMGVAFVLLNPTILLPGTWHEMRV
                     FAGEKRIGHDSYEFLGTLYRNRMTLWLTGSPWYFYYVFMGVKLPLTTVAAFVVGLPLL
                     FTKRLGDGRYFVLFWLFFWFFPFTFLGGKFTRYFTFPLPAVLITAAIGLSAAARAFAG
                     LVALFKESDAARLYARAALALVVVSLSAYAAGTAAPHYRLYTNAPLGGGSSRAGDYFP
                     HDEFYDASTRETVAEVVRRARPGARVASETPELLTHYAQVANRHDLVSVSLSDRAALR
                     DFAPGDVVVVARGRRYFSNDALTKALLGGFAADATVALGPVISTRIFVLDETTAALVS
                     NSAGG"
     gene            6782..8869
                     /locus_tag="AC159P_08275"
     CDS             6782..8869
                     /locus_tag="AC159P_08275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695488.1"
                     /translation="MNDAQMMHRPRKCSTTIASLLLLLTLGAPRVLRSQTAHDAPPSA
                     TAPTENAPQAAPVFRLEKIPVGRDAELLTVFGSLNGVRDGAAPAPDSGTASNSDVPLV
                     SVLRDTLGDEAPENDRLRYVWMLTYTRPGAWQRVASAVPFLYGRVGKGKKKSTSRGMP
                     PAVIDLAAPEREVWQRFMWMALQNVFFNPYGLAAKSTASTFHHNSESYRRAHIMRALA
                     ILSLYEAETGEQSAFSPSERIEIQGRLMLTGKSLGGLVDDSYLQRISHGNHVQTQDTR
                     GHNWELLRQRAEAEGLYFEPLTMPDGSATHALLWVARTDLEQNQERRFERRFLNIAKP
                     WGDTRLDKWRGHTETWHFDAESRVVAAGTEGARAVEMIPLALYGLDHPKIPSLLIDFR
                     DGANPKRREMSHRVLEDISRNLLALSRFGDVHYFLGRTVYDFVTSRRGMDINQPSRLR
                     AYSQLKLLLSLDASLDPTLRDEIGARIERVSLNPLENDLEAEAKLARDQHAALVAYAA
                     RPDGLAARLDRARREEMVALEHGKTERVLFGFARVATFGLYRHREHATPEEQRAALDR
                     ERQLAYHRRFLREVSKSAPFVEVAWNIEDVRRSLRFVSEQGAGADAATAAAAARIFAR
                     TNDEEVRRLCLNSLYRINNETAKKELLRIYQLPDLDPGTRLLTAEFLRRAVQEEQRIA
                     PADAKAIASVIGQ"
     gene            8866..>9473
                     /locus_tag="AC159P_08280"
     CDS             8866..>9473
                     /locus_tag="AC159P_08280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695489.1"
                     /translation="MIAATNKARGELRIATRRAKLTSGLARLFALAFLTALLSSSSLA
                     AGAKRVVVIKVDGLPHATVERYVRERDPRTGKSLLPWIEHVFYERGTRVSNFYVRGMS
                     LSGPAWSLLDTGQHLQIKANVEFDRLTLHSYDYLNFIPFWIGNVGGVRVDMPGTELMD
                     ELATPLLADAYPYDERFLSFQLYQRGTRWSTLERSLKNFFTTR"
BASE COUNT         1558 a         3366 c         2914 g         1635 t
ORIGIN      
        1 gtcgctggcc tgcggcttcg ccggctcgcc cgtgtaggtg atgacgacgc ggttcgtgtc
       61 gttgaaatac ttcttcatca cgcgctgcac gtccgccgcc gtcacggcct gaagcttcga
      121 gatgtcggag ttgacgcggc tggcgtcgcc catcagcacc gccgcgcggc ctatcgcgtc
      181 ggccttgccg ttgttcgtct cgcgctcgcg gagctggttc gtcacgagct ggttgacggc
      241 cttgtccagt tcggccttcg agaccggcgt gtcctgcatc agcttcagct ccgcgagcag
      301 cgcgcgctcg gcctccgccg gctgcttccc gctggcgagg atcgaggcga agtagacgag
      361 gccgttgtcc tcgcgcaaat cagccgtgac ccacgccgcc tgcgccacct gctgcttgta
      421 gacaagcgac tggtagaggc gcgaggattc gccagccgac aagatcgact cggccacgcg
      481 aatcgcgtag gcgtcgtcgc tcacgacggg gggcgcgaga taagtgatgg ccacggcggg
      541 cagcggcacg ttcgggccgg tctcgtcgaa gcgcttctcg gcggtgcgcg ccggctcttt
      601 cgcggtgacg cgcggcagcg gcgtgcccgg cttcggcacg cgaccgaagt acttgtcgat
      661 ccacgtgtcg agctgcttct gttcgaagtc gccgacgacg acgagcgtcg cgttgtcggg
      721 ccggtagaag gtcttgtgga aggcgcgcac gtcttcgagc gtggcggcgt cgaggttctc
      781 gatgctgccg atgccgggcc gccggtaggg gtgcgtcgtg taggaattct tctcgatgga
      841 gtagaggaac ctgccgtagg gcggcgcgag gatgcgcgag cggaactcct ccttcaccac
      901 gtcgcgctcg gaggggaagt tcttgtcgtt gacgttgagc gaacccatcc ggtcggcctc
      961 ggcccagagc agcgtctcaa ggtagttcga cggcacgacc tcgtagtagt tcgtgaagtc
     1021 gtcccacgtc gaggcgttgt tccagccgcc cacgtcctcg gtcagccgat ccatcatctc
     1081 gtccttcatg ttcttcgtcg atttgaacat gaggtgctcg aacaggtgcg cgaagccgct
     1141 ccggccctcg gggtcgtcct tcgacccgac cttataccag acctggatcg agacggtcgg
     1201 gctggacttg tcctgcacgg tgtagacctt catgccgttg gccagcgccc gctctttgaa
     1261 gttgatgggc ggcactgtcg tctgcgccgc cgcggacggg gcgaggcccg ccgcgagcgc
     1321 gccgcagagc agcactaacg cggtgcattg tcgaagcatc tttaatcgct cctctggttc
     1381 cttgaagaag ggccgccgag cggacggcgc ggagagtaac cctgcggtcg ctatgatgcc
     1441 cttcgggaga aaatctgttg tacgtttgag agggacgccg gcggcagtct aacgcccgtc
     1501 tccgaagaca agcaagcgcc ccgccgccgt tcgatttttg ttacgccgcg ccccttcaat
     1561 tagtttcgcg cgcgccccga aagtcgcaaa aaccttcggc attcgttcac cgaaatggat
     1621 aacggataac gggcgggaca agggggaaag atcgaaacga gggggaaagg ggaaagggcg
     1681 aaagggcgaa aaggaaagaa ccggcaggag gccgaacgga attctgtctt cacccttttc
     1741 gcctcttccc cctttcccct tttcccccgg ttttattctt tcgtcgcggg gacgcggaac
     1801 agcgcgaggc gttcgacgac gcgcccgccg acgatgtgct cttcgatgat ttcggccacg
     1861 tcgtcggcgt gcacgcgcgc gtaccagacg ccttcgggat agacgagcac cgccgcgccg
     1921 acggcgcaga agccgaccga gctgcaatcg gtcagcacca cgctccccaa agggttcttc
     1981 cccttcgcgg ccttgcccat gagcagcccg cgcgccgtca gctcgcggtc gaaagcctct
     2041 ttgacggcgg cgctgccgac cgccgtgcag gaagtgccgt tgcagaccag cacgtggtgg
     2101 cggatgggcg ccgcggtgac cggcgccagc gcgcacagtc tttcgcgttc gtcggtggtc
     2161 gaaacttctt ccattagaac attgtgttgt cgttggcaga actttccggc acgcgctgga
     2221 gcacgtccca atgttctacg accttgccgt ccgtatcgag ccggaaaata tcgatccctg
     2281 cccactcacc gtcgcccggc cagtgctggt agcaatgcaa gactacgtaa ttaccctccg
     2341 cgatcacgcg cttaaactcg acgcgctttt gcggatactc gctcgccatg cgggtaaagt
     2401 attcgatgaa ggcttccttg ccgtcggcta ctaccggatt gtgctggatg tacgtgtcgc
     2461 ccgtgtagcg ttcgatggct tcggcgggcc ggcactgatt gaacatcagg tcgtagaaag
     2521 ccgtcacggt cttcttgttc tgctcaagcc gctccatcat cctctccgaa tctacttttc
     2581 gcagaagtct tggcagactc cagtcaaaat cgaatcacgc ccctacacga cggcgacgct
     2641 gttttgcccg cgcagaatgt tagtataaaa tgcggctcgt ggcgaacacg cgtgggttcg
     2701 tgcgcggcgg cgagagccgg acgcggcccg gcgcgacgcc gctcaagttg aaaggttcaa
     2761 ggttcggctt catcgcagct ctatggcgaa cggcctggtg attcacatcg cgcgcgggca
     2821 ggatcggcac acagaggttc tgtcgcttga gcgcgtccgc atcggccccg gcgaagattg
     2881 ccacatcagg ctcgcgcccg acgccccgac ggactccacc ctgctcctcg aactcgcccg
     2941 caccaacgga aactaccgcg tcacggagtt cgacccggcc tccggcccga ccatcaacgg
     3001 cgtccccatc gagcacggcg acgccatcga ggacggcgac gagctgcgct tcgcgggcgc
     3061 cgccgacctc gccgtgcagt tcttccccgt ccgcgagctg ccggcggtcg tcgcgcaccg
     3121 ccgcgcgcag gtcgccccct tcatcgagca ggccgccatc gaggccgccg tcacggcccg
     3181 ccgcgacgac gccaaagtct tcctccgcga gttcacgcgc gagctgctgc gcgagatcaa
     3241 cctcaccacg aagatcggcg tggtgctcgt cacggtcatc ctcgtcggcg ggctgctcta
     3301 cctcggcaac gccgcgcgcc gcgagctgaa gcgcagccgc gacctgatcg acaagcagaa
     3361 cgaacagctc acgcggatgg gcgaggcgct cgacgggacg cggcagaagt tcgacgacgt
     3421 tgacaagaag agccaggaca tcatcgcgag cctctcgctc gcgccgcgtc tccggggcga
     3481 gtacggcaac ggcgtgtgcc tgatcgcagg ctcctaccag ctcgtcgagg ccgggacggg
     3541 acggcccctg cgctaccccg agacgcagca gaacgaggac ggctccgtca tcagcaacgg
     3601 cgccgagcag cccgtcctga cgcccgaagg caacggcgcc ccttacatcg cggacttcgt
     3661 cggcacgggc ttccacgtcg gcggcggcta catcgtcacc aaccgccatc tcgtcgtcga
     3721 gccgtggacg gccaacgacg gcgtgcagag tttgagcgcg agcgtcagcg ggcagttccg
     3781 cgtgacgcgc ctcgtcgcct tcttccccga ccacacgcag ccttacgtcc tcagggtcag
     3841 gcaggccacg gcgcgcgacg acctcgccgt cgggcagatc gacccggccc agatgcccgc
     3901 ggacgtgccc gcgctcccgc tcgacaagga ctcgggcgcc gccggcatcg gcaaggccgt
     3961 cgtgatgatg ggctacccga gcggcgagga gcggttgctg gcgacgctgc ccgagggcga
     4021 gtcgcgcagc atcatgcagc gctacggctc ctccatcgag acgctgctcg cgcacctcgc
     4081 cgagcgcaac tacatcaagc cgctgaccac gcaagggcac atcacggacg ttgacgcgcg
     4141 ccgccttgag ttcgacgcgc gcaacgccgt cggcggctcc ggctcgcccg tcttcggcca
     4201 gtcgggcggc gtcatcggcg tcaacttcgc ggccttcacg ggcatgcccg acgccaacta
     4261 cgccgtcccc gcgcgcttcg tctacccgct gctcgaacgc tccggctgga agagtcccga
     4321 gccggacgac aaggatacgg acgccaacgc gaacacgagc aacacgaagg acgcgaagga
     4381 ctcgcgcgtc gccacgacgc agacgaacca gtcgcgctga ggctgttttc acccccttcc
     4441 tcgttttgac acgacgcccc ccgtcggcta ccctttcgac ttttaatcgc ggtgccgcag
     4501 aggcttatga ccgccggcag gtgactgcga ccggagtaat gcttgtcggg gcagggcttg
     4561 tccctgcccg ctgacttccg acagactcga cttgaatttc aatcgaagcg cgggccgcgg
     4621 acttgccctg cccatacaag cgtcatgcag caatcgcatt cgaacacagg tagctgtagc
     4681 aactgagttc aggaataatc tttttcggag actctttatc actttgtctg catccgccgc
     4741 gacagtaacg accgccagca tcagaccaga gctttttccg gggcagatcg cgcgcccttc
     4801 gagccgcgcg atggtcgtcg cactcctcgc cttcctcgtc gtcgggggct tcggcctgcg
     4861 cgcctaccgc ctctccgccg aggcgctcag cgaagacgag ctgaacaagg tgcgcgccgt
     4921 cgccgactac cgcacgcgcg gcctgacgcc cgcgaacggc gagcacccgt tcctgatgaa
     4981 ggcgctgctg gcggcgagca ccgtcgcgtg cgaggcgtgg aacgcgacct ccatcgccga
     5041 ctcgaacccg cagctccgtg tctcgacgga gacggcggtg cgcctgcccg gcgcgctcct
     5101 cggcgcgctg accgcgctcc tcatcttcct cgtcgcctcc gagctgttcg gtttcgaggt
     5161 cggactcttc gccgccgcgc tgtgggcctt cgacccggcg gccatcggct tcaaccgcat
     5221 cgccaaggaa gacagcttcg tcgtcttctt cttcctcctt gccaacgtct tctggctgcg
     5281 cgggcagcgc gtggccgaag gcgggcacgc gcgcccggag ccttactact gggccgccgc
     5341 cgccgccttc ggcgcgatga tggcctcgaa gtacgtcccg ttctacatcg ccatcagcgg
     5401 cgcctactac tacgccttcc agggcgtcaa ggcgacgcgc tggcgcatgg gcaagaagaa
     5461 gtggctcgtc ttcttcgccg tcatgggcgt agcgttcgtc ctcctcaacc cgacgattct
     5521 gttgcccggc acgtggcacg agatgcgcgt cttcgcgggc gagaagcgca tcggccacga
     5581 ctcttacgaa ttcctcggca cgctctaccg caaccggatg acgctctggt tgacggggtc
     5641 gccgtggtat ttctactacg tcttcatggg cgtgaagctc ccgctgacga ccgtcgcggc
     5701 cttcgtcgtc gggctgccgc tgctcttcac gaagcggctc ggcgacggtc gctacttcgt
     5761 cctcttctgg ctcttcttct ggttcttccc cttcaccttt ctgggcggca agttcacgcg
     5821 ctatttcacc ttcccgctgc ccgccgtcct catcaccgcg gccatcggcc tcagcgccgc
     5881 ggcgcgggcg ttcgccggtc tcgtcgctct ctttaaagag agcgacgccg cgcggcttta
     5941 cgcccgcgcc gcgctcgcgc tcgtggtcgt ctcgctttcg gcctacgcgg cgggcaccgc
     6001 ggcgccgcac taccggctct acacgaacgc gcccctcggc ggcggatcga gccgcgcggg
     6061 cgactacttc ccgcacgacg agttctacga cgcgagcacg cgcgaaacgg tcgccgaagt
     6121 cgtccgcagg gcgcggcccg gcgcgcgcgt ggcgagcgag acgccggagc ttctgacgca
     6181 ctacgcgcag gtcgctaaca gacacgacct cgtctccgtc tctctctcag accgcgccgc
     6241 gctccgcgac ttcgcgcccg gcgacgtggt cgtcgtcgcg cgcgggcggc gctacttcag
     6301 caacgatgcg ttgacgaagg cgctgctcgg aggcttcgcg gcggacgcga ccgtcgcgct
     6361 tggccccgtc atttcgacgc gcatcttcgt cctcgacgaa acgacggcgg cgctcgtctc
     6421 gaactccgcg ggcggctgag cgaaaaatgt tgacaaccgc ccgcaccgct cgcggcatcg
     6481 cacgtgccaa cgaacagagg gcggcgggat tgcggatcgc ggatcgcgga tcgcggatta
     6541 cagcaacaaa agctgattga taacagcgtt gccttcatta tccgtcaacg cttcttctaa
     6601 tccgcaatcc gcaatccgca atcctcaatc gtctggggca cgcgccttgc acattagcct
     6661 tcgggtattt taaaaccgcg tgcgggcggc gttttttccg taccgtcttc attcatcatg
     6721 caaccgattc gctcctgttg gagtgaatcc gtacgagcac ggggccgcca acgaagagga
     6781 catgaacgac gctcaaatga tgcacagacc gcgcaagtgc tcgacgacaa tcgcttctct
     6841 cctcctcctt ctaacgctcg gcgcgccgcg tgtgctccgg tcgcagaccg cccacgacgc
     6901 gccgccgtcc gcaaccgcgc ccaccgagaa cgcgccgcag gccgcaccgg tcttccgtct
     6961 ggagaagatt ccggtcgggc gcgacgctga gctgctcacg gtcttcggca gtctgaacgg
     7021 cgtccgcgac ggcgccgccc cggctcccga ttcggggacg gcttccaatt ccgacgtgcc
     7081 gctcgtcagc gtgctgcgcg acacgctcgg cgacgaggcg cccgagaacg accgcctgcg
     7141 ctacgtctgg atgctcacct acacgcggcc cggcgcgtgg cagcgcgtcg cctcggcggt
     7201 gcccttcctc tacgggcgcg tcggcaaggg caagaagaaa tcgacctcgc gcgggatgcc
     7261 gcccgccgtc atcgacctcg ccgcgcccga gcgcgaagtg tggcagcgct tcatgtggat
     7321 ggcgctccag aacgtcttct tcaaccccta cggcctcgcc gccaagtcca ccgccagcac
     7381 cttccaccac aactccgaga gctaccgccg cgcgcacatc atgcgggcgc tcgccatcct
     7441 ctcgctctac gaggcggaga ccggcgagca gtccgccttc tcgccctcgg agaggatcga
     7501 gattcagggc cggctgatgc tcaccggaaa gtctctgggc ggcctcgtgg acgactccta
     7561 cttgcagcgc atcagccacg gcaaccacgt gcagacccag gacacgcgcg gtcacaactg
     7621 ggagctcctg cgccagcgcg cagaggccga gggtctctac ttcgagccgc tcacgatgcc
     7681 cgacggcagc gccacgcacg cgctcctctg ggtcgcgcgc acagacctcg aacagaacca
     7741 ggagcgcagg ttcgagcgcc gcttcctcaa catcgccaag ccgtggggcg acacgcggct
     7801 cgacaagtgg cgcggccaca cggagacgtg gcacttcgac gcggagagcc gcgtcgtcgc
     7861 cgcgggcacc gagggcgcgc gcgccgtcga gatgatcccg ctcgcgctct acggcctcga
     7921 ccacccgaag attccgtcgc tcctgatcga cttccgcgac ggcgccaacc cgaagcggcg
     7981 cgagatgtcg caccgcgtcc tcgaagacat ctcgcgcaac ctgctcgcgc tctcgcgctt
     8041 cggcgacgtg cactactttc tgggccgcac cgtctacgac ttcgtcacca gccgccgcgg
     8101 catggacatc aaccagccct cgcggctgcg cgcctactcg cagctcaagc tcctgctctc
     8161 gctcgacgcc tcgctcgacc cgactttgcg cgacgagatc ggcgcgcgca tcgaacgcgt
     8221 ctcgctgaac ccgctggaga acgacctcga agccgaggcc aaactcgcgc gcgaccagca
     8281 cgctgcgctc gtcgcctacg ccgcgcgacc ggacggcctc gcggcgaggc tcgaccgcgc
     8341 acgccgcgaa gagatggtcg cgctcgaaca cggcaagacc gagcgcgtgc tcttcggctt
     8401 cgcgcgcgtc gccaccttcg gcctctaccg gcatcgcgag cacgccacgc ccgaagagca
     8461 gcgcgccgcg ctcgaccgcg agcggcagct cgcctaccac cgacgcttcc tgcgcgaggt
     8521 ctcgaagtcc gcgcccttcg tcgaggtcgc ctggaacatc gaggacgtgc gccgctcgct
     8581 ccgcttcgtc tcggagcagg gcgcgggcgc cgacgccgcc accgccgccg ccgccgcgcg
     8641 catcttcgcg cggaccaacg acgaagaggt gcgcaggctc tgtctcaatt cgctctaccg
     8701 catcaacaac gagacggcga agaaggagct gctgcggata taccaactcc ccgacctcga
     8761 ccccggcacg cgcctcctga ccgccgagtt cctccgccgc gccgtgcagg aagaacaacg
     8821 catcgccccc gccgacgcca aagccatcgc gtccgtcatc ggccaatgat agctgcgacg
     8881 aacaaagcga gaggcgagct acgaatcgcg acgcgccggg cgaaacttac gtccggcctg
     8941 gcgcggctct tcgccctcgc gtttctgaca gcactcctct catcatcctc tctcgctgcc
     9001 ggggcgaagc gcgtcgtcgt catcaaggtt gacggcctgc cgcacgccac ggtcgagcgc
     9061 tacgtccgcg agcgcgaccc gcgcaccggc aaaagcctgc tcccgtggat cgagcacgtc
     9121 ttctacgagc gcggcacgcg cgtctcgaac ttctacgtcc gcgggatgag cttgtccggt
     9181 cccgcctggt ctttgctcga tacggggcag cacttgcaga tcaaggccaa cgtcgagttc
     9241 gaccggctca cgctccactc ctacgactac ctcaacttca tccccttctg gatcggcaac
     9301 gtcggcgggg tgcgcgtgga catgcccggc accgagctga tggacgagtt ggcgacaccg
     9361 ctcctcgccg acgcctaccc ttacgacgag cgcttcctca gcttccagct ctaccagcgc
     9421 ggcacgcgtt ggagcacgct cgaacgcagc ctcaagaact tcttcaccac gcg
//
LOCUS       DBNGOH010000421         4736 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2080772, whole genome shotgun sequence.
ACCESSION   DBNGOH010000421 DBNGOH010000000
VERSION     DBNGOH010000421.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4736)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4736)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4736
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2080772"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1146
                     /locus_tag="AC159P_08285"
     CDS             <1..1146
                     /locus_tag="AC159P_08285"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HIR3695490.1"
                     /translation="KRPQTTSFTYALPRFLSGSARQLQRRGRRGAEGAEKTDCRFEIS
                     NSESPISSLCSLRVLCALCVNNLNARFLSTVVQTLLVLVTLALINAPPAPAQKKGATP
                     KAAAQTVTLKTHPNAAVWLDDVRRGTTDAQGSLEMKVSPGVHRLRVRAGGYAEQSFPL
                     LPAQRGLVSITLRPTKDEAELAFQQAEEQREKGGGEEGRARAVELYRKALKLRPRFPA
                     AHVGLARVLLSQEQHDAALAEIAAARRDRPGYAEASAVEGRVLRGIPDEEGAIASFQR
                     SIREARGFQPEAHAGLGLVLEERGDHESAVNSFRRAIAQLSDTEPVLYEFLGRNLEKL
                     ERWKDAVAAYEKYLELAPTGSHASAINSIIDQLRQQAAEAEQQSTPP"
     gene            complement(1166..1531)
                     /locus_tag="AC159P_08290"
     CDS             complement(1166..1531)
                     /locus_tag="AC159P_08290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695491.1"
                     /translation="MLRIVLRVLAVLFLAGGLLGSAIAGYLLLTPSDEQLSYEQKYRE
                     MNEKYAKARAAKDPVEKARLLKEAEDAAGWAKEWGEGARTRRVWHLVGAGVGVAVVFV
                     SFVVFLLTFVGRKAGAAPA"
     gene            complement(1612..4548)
                     /locus_tag="AC159P_08295"
     CDS             complement(1612..4548)
                     /locus_tag="AC159P_08295"
                     /inference="COORDINATES: protein motif:HMM:NF024627.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007154 - cell communication [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Calx-beta domain-containing protein"
                     /protein_id="HIR3695492.1"
                     /translation="MQDAGRRHRRSVILSFVLLSLTGWFALPGHFRNRVAPTASAQTG
                     AFTVNTTNDTNDGVCTIAHCSLREAINAANNSTSRLNTIRFDIGGTSHVKTISPASAL
                     PAVNGQVVIDGTTQPGYAGTPLVELNGAGAGAGANGLTLKGEGSTVKGLAVNRFGVGI
                     SLGGNGGHTVRGNYLGTNAAGATGIGNVVGVAMAGSAANNTVGGATAAERNVISGNRG
                     NGISIRDAGSTGNKVQGNFIGTDPSGNSILANSGTGIRIDLDAANNVIGGASVGERNV
                     ISGNGSSGIFIAGAHGNQVEGNYIGTNAAGTGGLGNTFSGVHIGADSSSNSVKSNTIA
                     FNTREGVFVQSGTANHVVSNAIHSNGGLGIDLGADGVTPNDAGDGDAGANNLQNFPVL
                     TSATYAGGRLAIAGTHDRSAGFYSYEFFASASCDPSGNGEGERLITTFGGGGSNGGIT
                     NFVIGRTAPLPAGRFITATATRVNELFENPSDTSEFSPCVRITMPGSLQFSAAAYNAD
                     EGAGTAAVSIDRVGGSEGATTVFFITSDGTATAGQDYTDTEIPVSFAAGETSKTVLVP
                     IADDNSPEGSETVNLTLLQATGGAPVGTPSAAVLTINDNDSGSVFHLSDSNYGASEDC
                     AAVNVIVHRVGDTSGTSTVSYATASGTASERADFNAALGTLRFNPGETEKSFVLLVNE
                     DSHTDGTETASVTLSNPANASLGGPSTATLSIFDDASEPAANASDDSAKFVCQHYHDF
                     LNRQPDAGGQAFWTNEIEQCGASAECREAKRVNVSAAFFLSIEFQETGYLVYRMYKAA
                     YGNLVGAPVPLTLPEFLPDTQRIGQGVEVGVGDWQARLAANKAAFAGEFVARARFTTA
                     YPAGMSAAQFVDTLNANSGGALSPGERNQLVAELTAGAKSRAEVLRAVAEDETLAQSE
                     SNRAFVLMQYFGYLRRSPNSAPDTDYGGYTFWLQKLNQFGGNFVNAEMVKAFISSIEY
                     RRRFGQ"
BASE COUNT          730 a         1645 c         1544 g          817 t
ORIGIN      
        1 aagcgaccgc agacgacttc atttacttac gccctgccaa gattcctgag tggaagcgca
       61 cgtcagcttc aacgcagagg gcgcagaggc gcagagggcg cagagaaaac cgattgcaga
      121 ttcgagattt cgaattccga atctccgatt tcttctctgt gctctctgcg tgtcctctgc
      181 gccctctgcg ttaacaacct caacgcacgc ttcctctcaa ccgtcgtcca aaccctcctc
      241 gtcctcgtca ctttagctct catcaacgcg ccgcccgcgc ccgcgcaaaa gaagggcgcg
      301 acgccgaaag ccgccgcgca gaccgtcacc ctcaagacgc accccaatgc cgccgtctgg
      361 ctcgacgacg tgcgccgcgg cacgaccgac gcccaggggt cgcttgagat gaaggtctcg
      421 cccggcgtcc accgcctgcg cgtccgcgcc ggcggctacg cggagcagtc tttcccgctg
      481 ctccccgcgc agcgcggcct cgtctccatc acgctccgcc ccacgaagga cgaggccgag
      541 ctggccttcc agcaggccga ggagcagcgc gaaaagggcg gcggcgaaga gggccgcgcg
      601 cgcgccgtcg agctttaccg caaggccctc aagctccggc cccgcttccc cgccgcgcac
      661 gtcggcctcg cccgcgtcct gctctcgcag gagcagcacg acgccgcgct cgccgagatc
      721 gccgcggcgc ggcgcgaccg ccccggctac gccgaagcct ccgcggtcga gggccgcgtc
      781 ctgcgcggca tccccgacga agagggcgcc atcgcctcct tccaacgctc catccgcgag
      841 gcgcgcggct tccagcccga ggcgcacgcc ggcctcggcc tcgtcctcga agagcgcggc
      901 gaccacgaga gcgccgtcaa ctccttccgc cgcgccatcg cgcagctctc cgacaccgaa
      961 cccgtgctct acgaattcct cggcaggaat ctggagaagc tcgaacgctg gaaggacgcc
     1021 gtcgccgcct acgaaaagta tctcgaactc gcgcccaccg gcagccacgc cagcgccatc
     1081 aactccatca tcgaccagct ccgccaacag gccgccgagg cagagcagca atccacgccg
     1141 ccctgacttc gaacggcgcc ctgactcaag cgggagccgc gccggccttg cgtccgacga
     1201 aggtcagcag gaaaacgacg aagctcacga acaccaccgc gacgccgacg cccgcgccga
     1261 cgaggtgcca cacccgtctg gtgcgggcgc cctcgcccca ctctttggcc cagccggcgg
     1321 catcctccgc ttctttcaac agccgtgcct tctcgaccgg gtctttcgcg gcccgcgcct
     1381 tcgcatactt ctcgttcatc tcccggtact tctgctcgta gctcaactgc tcgtcgctcg
     1441 gcgtgagcag caggtagccg gcgatggccg agccgagtag tccgcccgct aagaacaaca
     1501 cggcgagcac gcgcagcacg attctcaaca tgactcgcac ctccgtcggg ccgcaggccg
     1561 gccccgcacg ttctccttcg gggccggcga ctgaatggtc gttagaggac ttcactggcc
     1621 gaagcgccgg cggtactcga tggacgagat gaatgccttg accatctcgg cgttgacgaa
     1681 gttgccgccg aactggttga gcttctggag ccagaaggtg tagccgccgt agtccgtgtc
     1741 cggcgcgctg ttcgggctgc gcctgaggta gccgaagtat tgcatcagca cgaacgcgcg
     1801 gttcgattcg gactgcgcga gcgtctcgtc ctcggccacc gcgcgcaaga cctcggcgcg
     1861 cgacttcgcc cccgcggtca gttcggcgac cagctggtta cgctcccccg gtgagagggc
     1921 gcccccgctg ttcgcgttca acgtatcgac gaactgcgcc gcgctcatcc ccgccgggta
     1981 cgcggtggtg aagcgtgcgc gcgcgacgaa ctcgcccgcg aaggccgcct tgttggccgc
     2041 gagccgcgcc tgccagtcgc cgacgccgac ctcgacgccc tgcccgatac gctgcgtgtc
     2101 gggcaggaac tccggcagcg tcagcggcac gggcgcgccc accaaattgc cgtaggccgc
     2161 cttgtacatc cggtagacga ggtagcccgt ctcctgaaac tcaatcgaga ggaagaaggc
     2221 cgccgagacg ttgacgcgct tggcctcgcg gcactcggcg ctcgccccgc actgctcgat
     2281 ctcgttcgtc cagaaggcct gcccgcccgc gtccggctgg cgattcaaaa agtcgtggta
     2341 gtgctggcag acgaacttcg ccgagtcgtc cgaggcgttc gccgcaggct ccgacgcgtc
     2401 gtcgaagatc gagagcgtcg cggtcgaggg gccgcccaag gaagcgttcg ccgggttcga
     2461 gagcgtgacg gaggccgtct ccgtcccgtc cgtgtgcgag tcctcgttga cgagcaggac
     2521 gaaggacttc tctgtctcgc cggggttgaa gcggagcgtg ccgagagccg cgttgaagtc
     2581 ggcgcgctcc gaggccgtgc cgctcgccgt cgcgtagctg accgtggatg tgccggaggt
     2641 gtcgccgacg cggtggacga taacgttgac ggcggcgcag tcctcgctcg cgccgtagtt
     2701 ggaatccgag aggtggaaga cgctccccga atcgttgtcg ttgatggtca gcacggcggc
     2761 gctcggggtg ccgacgggcg cgccgcccgt cgcctgcaaa agcgtgaggt tgaccgtctc
     2821 gctgccctcg ggtgagttgt cgtcggcgat ggggacgagc acggtcttgc tcgtctcgcc
     2881 agcggcgaag ctgaccggga tttcggtgtc ggtgtagtcc tgcccggcgg tggccgtgcc
     2941 gtccgaggtg atgaagaaga ccgtcgtcgc gccttcgctg ccgcccacgc ggtcgatgct
     3001 gacggcggcg gtgcccgcgc cttcgtcggc gttgtaggcc gcggcgctga actgcaagct
     3061 gccgggcatg gtgatccgaa cgcacgggga gaactccgac gtgtcggaag ggttctcgaa
     3121 caattcatta actctggtgg cggtggcggt gataaaccgg cccgctggca gcggggcggt
     3181 tctgccgatg acgaagttgg tgatgccgcc gttgctgccg ccgccgccga atgttgttat
     3241 taacctttcg ccctcgccgt tgccggatgg gtcgcaggag gcgctggcga agaactcgta
     3301 cgagtagaag ccggcggatc tgtcatgcgt ccccgcgatg gccaggcgtc cgccggcgta
     3361 agtcgccgag gtcagcacgg ggaagttttg caggttgttc gcgcccgcgt cgccgtcgcc
     3421 cgcgtcgttc ggcgtgaccc cgtccgcgcc gaggtcaatg ccgaggccgc cgttggagtg
     3481 gatcgcgttc gagacgacgt ggttggcagt gccggattgg acgaacacgc cttcgcgcgt
     3541 gttaaaggcg atggtgttgg atttgacgct gttggaggag gagtcggcgc cgatgtgaac
     3601 gccgctgaaa gtatttccca agcccccggt gcctgccgcg ttcgtgccga tgtagttacc
     3661 ctctacctga ttgccgtgcg cccccgcgat aaaaatgcca ctgctgccgt tgccggagat
     3721 gacgttgcgc tccccgacgc tcgccccgcc gatgacattg ttcgcggcgt cgagatcgat
     3781 gcgaatgcct gtgccgctgt tcgccagtat ggagttgccg gacgggtcgg tgccgatgaa
     3841 attgccctgc accttgtttc ccgtcgagcc ggcgtcgcgg atgctgatgc cgtttccgcg
     3901 attgcccgag atgacgttcc tctccgcggc ggtcgcgccc ccgaccgtgt tgttggcagc
     3961 gctgcctgcc atcgccacgc cgaccacgtt cccgatgccc gtcgcgccgg cggcgttcgt
     4021 gccgaggtag ttgccccgca cggtgtggcc gccgttccca cccaaactga tgccgacgcc
     4081 gaagcggttg acggcgagtc ctttcacggt gctgccctcg cccttgagcg tcagcccgtt
     4141 cgcgcccgcc cccgcgccag ccccgttcaa ctcgacgagc ggcgtgccgg cgtagccggg
     4201 ctgcgtggtg ccgtcgatga cgacctgccc gttgaccgcg ggcagcgcgc tcgccgggct
     4261 gatcgtcttg acgtggcttg tgccgccgat gtcgaagcga atggtgttga gtctggacgt
     4321 ggagttgttg gcggcgttga tggcctcccg caggctgcaa tgggcgatgg tacacacgcc
     4381 gtcgttggtg tcgtttgtcg tgttgacggt gaacgcgccg gtctgcgccg aggccgtcgg
     4441 cgccacacgg ttccggaagt gaccgggcag ggcgaaccag cccgtcagcg acagaagcac
     4501 gaacgacaga atgaccgagc gcctgtggcg tctccctgcg tcctgcatgg tcttactcct
     4561 tccactggcc gcaacccccg cggccacacg catagccggc gcctctcctt cacactgaaa
     4621 ccgagcgaga ccgggtcggt catcacgggc agccgacgat ctcgtcttcg gagagtttgg
     4681 cgaaggtctg accgtaggtg cctccgccgg cgtaatcctg ctgcacgtgg atggtg
//
LOCUS       DBNGOH010000422         4024 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3180484, whole genome shotgun sequence.
ACCESSION   DBNGOH010000422 DBNGOH010000000
VERSION     DBNGOH010000422.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4024)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4024)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4024
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3180484"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..922
                     /locus_tag="AC159P_08300"
     CDS             <1..922
                     /locus_tag="AC159P_08300"
                     /inference="COORDINATES: protein motif:HMM:NF019869.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HIR3695493.1"
                     /translation="PNEVIKESGAEILRLWCTSSDYQEDMRCSPEILQRAQDAYRKMR
                     NTARFALGNLDGFDPVRDAVGEHEMEEIDRWALAELDAVAARAVAAYKAFEYHVVHHA
                     LYNFCTVTLSARYVDIIKDRLYTSAPKSRARRSAQTALHRITDALARLLAPVLVFTAD
                     EIWESLPRTGADAGLQSVHLALFPAAPVGERDAALRAQWTQLFEVRDVVLRALEAARV
                     SKLIGSSLEAHVRLKAKTRRDYELLESRREQLRYLFIVSQVSLTFADEGAGAEGEPVE
                     VEVVRADGEKCERCWNYSKRVGEFTRYPTV"
     gene            976..1548
                     /gene="lspA"
                     /locus_tag="AC159P_08305"
     CDS             976..1548
                     /gene="lspA"
                     /locus_tag="AC159P_08305"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HIR3695494.1"
                     /translation="MNGSGNSESSSGAGGQRRNLVWRVAYLLAAAGVYLTDQASKAWA
                     VRELRFGSTRTVIDGVFYFSYAENPGIAFGQLQGGGQFGRWMLATLAALAAVGVVLYL
                     FRTTRTDDRVLGACALLLAGIAGNLTDRVRLGHVIDFIEVHIGSFQWPTFNVADAVIC
                     VGAGLLALDLFLEGRRQEVPKAVTSDELKS"
     gene            1564..2181
                     /locus_tag="AC159P_08310"
     CDS             1564..2181
                     /locus_tag="AC159P_08310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695495.1"
                     /translation="MDKLLTLLLLVVLAGIAPAAFAQGGASGRPTAAPENLSEPSSKH
                     ALYRRFLDNFKDNQPEAVKAAKLYMEKYAADDDEIVRHLKKWVDRYEKATGADGFYLA
                     NIACGVRMHEKTYLVRRGGTLRHTVWSYESPELAEAALEKKFKSAEVVERRPILDEQG
                     VKVGERVVVKKAERALVLRTGKGLIVGIEAPSVEVLEEFEKVRRR"
     gene            2232..3077
                     /gene="lgt"
                     /locus_tag="AC159P_08315"
     CDS             2232..3077
                     /gene="lgt"
                     /locus_tag="AC159P_08315"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HIR3695496.1"
                     /translation="MFPELFRIGSFPVNTYGVLMAVAFLVALFVASRLAERDGLKRER
                     VFDMGLWMLLAGLIGSKLLLLLAEAEYRADPSKLLSLDFLRSGGVWYGGFLGGLAAGV
                     ILMRRYGLPFWKTADAFAPGVAIGQSIGRLGCFAAGCCWGRECQLPWAVTFPERANEI
                     TGVPAGVHLHPTQVYEALTMLVIFLLLVWLHRRKSFDGQVLLTYAALYGAARFVIEFF
                     RDDPRGDILGLTTLTGLSTSQLISLFVGLGGLVFLFIRRRRARGDAAAGGRDAAASAS
                     DTAQA"
     gene            3081..3281
                     /locus_tag="AC159P_08320"
                     /pseudo
     CDS             3081..3281
                     /locus_tag="AC159P_08320"
                     /inference="COORDINATES: protein motif:HMM:NF013633.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
     gene            3599..>4024
                     /locus_tag="AC159P_08325"
     CDS             3599..>4024
                     /locus_tag="AC159P_08325"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HIR3695497.1"
                     /translation="MVHRLDRDTSGLIVVAKSAFAHEHLAEQFRARTVFKSYAALVHG
                     RLKEDAGRVEQPLARDPRHRTRMAVVRGGREAVTVWRARRRYDRFTLLDVVIKTGRTH
                     QIRVHLAWLKHPVVGDETYGMGRDRNLADARLRAALAALG"
BASE COUNT          652 a         1340 c         1390 g          642 t
ORIGIN      
        1 gccgaacgag gtcatcaagg agtcgggcgc ggaaatcctg cggctctggt gcacgtcgtc
       61 ggactatcag gaggacatgc gctgctcgcc ggaaatcttg cagcgcgcgc aggacgccta
      121 ccgcaagatg cgcaacacgg cgcgcttcgc gctcggcaac ctcgacgggt tcgaccccgt
      181 gcgcgacgcc gtcggcgagc acgagatgga ggagatcgac cgctgggctt tggccgagct
      241 ggacgcggtc gcggcgcgcg ccgtcgcagc ctacaaggct ttcgagtacc acgtcgtcca
      301 ccacgcgctc tacaacttct gcacggtgac gctgtcggcg cgctacgtcg acatcatcaa
      361 ggacaggctc tacacgtctg cgccgaagtc gcgcgcgcgg cgctcggcgc agaccgcttt
      421 gcaccgcatc acggacgcgc tcgcgcgcct tttggcgccc gtcctcgtct tcacggcgga
      481 tgaaatctgg gagagcctgc cacgcacggg cgcggacgca ggacttcagt cggtgcatct
      541 cgcgctcttc ccggcggcgc ccgtcggcga gcgcgacgcc gctttgcgcg cgcagtggac
      601 gcagctcttc gaggtgcgcg acgtggtgtt gcgcgctctg gaagcggcgc gcgtctcgaa
      661 gctcatcggc agcagcctcg aagcgcacgt gcgcctgaag gcgaagacgc ggcgcgacta
      721 cgagctgctc gaaagtcgcc gcgaacagct ccgctacctg ttcatcgtct cgcaggtctc
      781 gctgacgttc gcggacgagg gggcgggcgc cgaaggcgag ccggtggagg tcgaggtcgt
      841 gcgcgccgac ggcgagaagt gcgagcggtg ctggaactac tcgaagcgcg tcggcgagtt
      901 cacgcgctac ccgacggtgt gagagcgctg cgtcgaggcg ctcgcggaga tcgaggccga
      961 gggggggggc gcgtcgtgaa cggtagcggc aacagcgagt cgagcagcgg cgcgggcggg
     1021 cagaggcgca acctcgtctg gcgcgtcgct tacctcctgg cggcggcggg cgtctacctg
     1081 accgatcagg cgagcaaggc gtgggcggtg cgcgagctgc gtttcggcag cacgcggacg
     1141 gtcatcgacg gcgtgttcta tttctcctac gcggagaacc ccggcatcgc cttcgggcag
     1201 ttgcagggcg gcggccagtt cggccggtgg atgctcgcga cgctggcggc gctcgcggcg
     1261 gtgggcgtcg tcctgtacct cttccgcacg acgcggacgg acgaccgcgt gctcggcgcc
     1321 tgcgcgctgc tgctcgcggg gatcgccggc aacctgaccg accgcgtccg cctcggccac
     1381 gtcatcgact tcatcgaagt ccacatcggc tcgttccagt ggccgacctt caacgtggcc
     1441 gacgccgtga tctgcgtcgg cgcggggctg ctcgcgctcg acctgtttct ggaaggcaga
     1501 aggcaagaag tgcccaaagc cgtgacaagt gacgaattga aaagctgagc ggaggatcgg
     1561 caagtggata aactactaac tctgctcctg ctggtcgtgc ttgcggggat cgctcccgcg
     1621 gcgttcgcgc agggcggcgc ttctggtcgg ccaacagccg cccccgaaaa tcttagcgag
     1681 ccttcttcaa agcacgccct ctaccggcgg ttcctggaca atttcaaaga taaccagccg
     1741 gaggccgtca aggcggcaaa gctgtacatg gagaagtatg ccgccgatga cgacgagatc
     1801 gtcagacatc tgaagaagtg ggtggacaga tacgaaaagg cgaccggtgc cgacggcttc
     1861 tatctcgcta acatcgcatg cggagtacgg atgcacgaga aaacttatct cgtgcgccgt
     1921 ggcgggacgc ttcgtcatac ggtgtggtcg tatgagtcgc ctgagcttgc cgaagccgcg
     1981 ctggagaaga agtttaagtc ggcagaagtc gtcgagcgac ggccaatact ggacgagcaa
     2041 ggagtcaagg tgggcgaaag ggtagtcgtg aagaaggcgg agagggcgct ggtgctccgg
     2101 acaggtaagg gactgatagt gggcatcgag gcgccgtctg tcgaagttct cgaggagttt
     2161 gagaaagtga gaaggcgctg accggaagcg tgcagcccgg cctaggcatg gtctgctcgt
     2221 cacttgaaat aatgttccca gagcttttcc gcatcggcag cttcccggtc aacacctacg
     2281 gcgtgttgat ggcggtggcg tttctggtgg cgctgttcgt ggcctcgcgt ctcgcggagc
     2341 gcgacgggct gaagcgcgag cgcgtgttcg acatggggct gtggatgctc ttggcggggc
     2401 tgatcgggtc gaagctgctg ctgctcttgg ccgaggccga gtaccgcgcc gacccttcaa
     2461 agctgctgtc gctcgacttc ctgcgctcgg gcggcgtgtg gtacggcggc ttcctcggcg
     2521 gcctcgccgc gggcgtcatc ctgatgcgcc gctacgggct gccgttctgg aagacggcgg
     2581 acgcgttcgc gccgggcgtc gccatcgggc aatcgatcgg gcggctcggc tgtttcgccg
     2641 cgggctgctg ctggggccgt gagtgtcaac tgccgtgggc cgtcacgttc cccgagcgcg
     2701 cgaacgagat cacgggcgtg cccgcgggcg tccacctgca cccgacgcag gtctacgaag
     2761 cgctgacgat gctcgtcatc ttcctcctgc tggtgtggct gcaccgccgc aaaagtttcg
     2821 acgggcaggt gctcctgacc tacgccgcgc tctacggcgc ggcgcgcttc gtgatcgagt
     2881 tcttccgcga cgacccgcgc ggcgacatcc tcggcctgac gacgctgacc ggcctctcca
     2941 cctcgcagct catcagcctc ttcgtcggcc tcggcggact cgtcttcctc ttcatccgcc
     3001 ggcgccgcgc gcgcggcgac gcggcagcag gaggccgtga cgccgccgcc tctgcctccg
     3061 acacggcgca ggcgtgaggc atgagcgacg aacacttcgc cgagccggag accgaaacgc
     3121 tcgccttcga ggtctccgaa gaggaagcgg gcacgcggct cgacgcgctg ctcgccgcgc
     3181 gcgtcgaagg cgtgagccgc acggcgctca agcgcctcat cgacgaaggc gaagtgctcg
     3241 tcgcgggacg cgccgccaag ccctcgcaca aagtccgcgc gggcgaccgc gtcgaagtcg
     3301 aactcgtcgc gcccccgccg ctcgacctcg cccccgaaga catcccgctc gacatcgtcc
     3361 acgaagacga agaggtcgtc gtcgtcaaca aacccgccgg gatggtcgtc cacccggcgg
     3421 caggagtccg cggcggcacg ctcgccaacg cgctcgccca tcgcttcgcg caattgcgga
     3481 tcgcggatcg cggattgcgg agaagcaaag cggcccttcg acagaggcgc caagacctgc
     3541 gctcgaaaac acgaatccgc aatccgcgat ccgcaatccg caatcggttc ggcccggcat
     3601 cgtccaccgc ctcgaccgcg acacgtcggg cctcatcgtc gtcgccaaga gcgccttcgc
     3661 gcacgaacac ctcgccgagc agttccgcgc gcgcacggtc ttcaagtcct acgccgcgct
     3721 cgtccacggg cggctgaagg aggacgcggg gcgcgtcgag cagccgcttg cgcgcgaccc
     3781 gcgccaccgc acgcggatgg ccgtcgtgcg cggcgggcgc gaggccgtca ccgtctggcg
     3841 cgcgcgccgc cgctacgacc gcttcacgct gctcgacgtg gtcatcaaga ccgggcgcac
     3901 gcaccagatt cgcgtccacc tcgcgtggct caagcacccg gtcgtcggcg acgagaccta
     3961 cggcatgggc cgcgaccgca acctcgcgga cgcgcgcctg cgcgccgccc tcgccgccct
     4021 gggt
//
LOCUS       DBNGOH010000423         2933 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3730744, whole genome shotgun sequence.
ACCESSION   DBNGOH010000423 DBNGOH010000000
VERSION     DBNGOH010000423.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2933)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2933)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2933
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3730744"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..410
                     /locus_tag="AC159P_08330"
     CDS             <1..410
                     /locus_tag="AC159P_08330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695498.1"
                     /translation="PPSKKRDDRHYLRVSHWLTLVWGIVQIGAALLAMRQNRSALDQA
                     LSIASLINGPVLGVFLVGTFLRRVSQTPALIGMVTSIVVMLYIRFFTPLAWTWYVLVG
                     SAVTFLVAWLAGYAFAPAPAEQRDELAPNAAAD"
     gene            846..2579
                     /locus_tag="AC159P_08335"
     CDS             846..2579
                     /locus_tag="AC159P_08335"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013126.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 protein"
                     /protein_id="HIR3695499.1"
                     /translation="MSLAEKAGQLIAVGVNARFLNRESDEYRALRRQVEENHVGGIIL
                     FRGPVYESVHLVNRMQQHARLPLLISADLEAGAGMRFEDTVNLPWNMAVGATGNTDLA
                     RRQGELTAKEARALGVQQIFAPVVDVNNNAANPVINVRSYGEDPAEVARFAAAFTEGA
                     QSAGVIATAKHFPGHGDTNVDSHRGLPVISYKRERLEQIELVPFRAAIKAGVGAVMSA
                     HIGLPEIDPTTVQPLPRDAMVRPAYVEEGGEIVDEKTYLPGTLSPAVLSGLLRRDLGF
                     DGIVVTDALDMSGLTIYFKQDEAAVRAVLAGADMLLKPADPDAVARGLREAVASGRLA
                     EKRIEESARKILAAKYDLGLVKQRTAPLELLDQLVSSPETLKLADDVARGAVTLVRDD
                     ARLLQRTDGAGLRPGARLFNLAITNGEDRFFIANAFAGQLARSGRRAETVVLDGRSTE
                     EEVKAAIEKAQKADVVIASMYGRVRSGAANSVGLPEQGARVLSTLLERKVPVVGVSFG
                     NPYLLQSFPALQTYVVAYGDMPSLQRAVARALLGQADITGRLPISLPGLYPRGTGIQL
                     KASATPATAIK"
     gene            2685..>2933
                     /locus_tag="AC159P_08340"
     CDS             2685..>2933
                     /locus_tag="AC159P_08340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695500.1"
                     /translation="MICLLTAQASATTLKVVPPEAVGMSAKHLAHIDGAVAGSIERGE
                     TPGAVVLVARRGGVVWRKAYGARAVVPRREEMTVDTVFD"
BASE COUNT          489 a         1063 c          939 g          442 t
ORIGIN      
        1 cgccgcccag taagaaacgc gacgaccggc attacctccg cgtctcgcac tggctgacgc
       61 tcgtgtgggg catcgtgcag atcggcgcgg cgctgctggc gatgcggcag aaccgctcgg
      121 cgctcgacca ggcgctctca atcgcctcgc tcatcaacgg ccccgtgctc ggcgtcttcc
      181 tcgtcggcac cttcctccgg cgcgtgagcc agacgcccgc gctcatcggc atggtcacga
      241 gcatcgtcgt catgctctac atccgcttct tcacgccgct cgcgtggact tggtacgtgc
      301 tcgtcggcag cgccgtgacg ttcctcgtcg cgtggctcgc cggctacgcc ttcgcgcccg
      361 cgcccgctga gcagagagac gaactcgcgc cgaacgcggc ggctgattaa atcgattcgc
      421 gcgcgccata cgccgcgtcg cggcgactga atctagcccg gcgtttcaac accgggtcac
      481 agagcaggtt tccatctgcg ccgcgtcggc gacgcctgag cgcgggccgt gagattcacg
      541 cgtcgctacg cgacgccgaa ccgagggccg tgcaaacccc ggccttgaaa ggccggacta
      601 aactcaaacg ccgctccgcg gcgatagata aaatcgaaca tcaacaggca gtgagagctt
      661 taatgagaac cagtattcaa ccactccggg tcgcgctcgc actcgttcta tttgtcgctt
      721 cgacgctcgc gcctttgtcc actccggtgc atggtcgcag cgcacacctt acttcaccgc
      781 gcgagtcgta ctcacgccgc gcttcaggtg acgcgctcaa gtgggccgac gcggaattga
      841 agaagatgtc gcttgccgag aaggcggggc agctcatcgc ggtcggcgtc aacgcgcgct
      901 tcctcaaccg cgagagcgac gagtacaggg ctttgcgccg gcaggtcgaa gagaaccacg
      961 tcggcggcat catcctcttc cgcggccccg tctacgagtc cgtgcatctc gtcaaccgca
     1021 tgcagcagca cgcgcgcctt ccgctgctca tctccgcaga cctcgaagcc ggggcgggga
     1081 tgcgtttcga ggacacggtg aacctgccgt ggaacatggc cgtcggcgcg accggcaaca
     1141 ctgaccttgc gcggcggcag ggcgagctga cggcgaagga ggcgcgcgcg ctcggcgtgc
     1201 agcaaatctt cgcgcccgtc gtggacgtga acaacaacgc cgccaacccc gtcatcaacg
     1261 tccgctccta cggcgaagac cctgcggagg tcgcgcgctt cgccgccgcc ttcaccgagg
     1321 gcgcgcagag cgcgggcgtc atcgccaccg ccaagcactt ccccggccac ggcgacacga
     1381 acgtcgattc gcaccgcggc ctgcccgtca tcagctacaa gcgcgaacgg ctcgaacaga
     1441 tcgaactcgt ccccttccgc gcggcgatca aggcgggcgt cggcgccgtc atgtccgcgc
     1501 acatcggcct gcccgagatc gacccgacga ccgtccagcc cttgccgcgc gacgcgatgg
     1561 tcagacccgc ctacgtcgaa gagggcggcg agatcgtgga cgagaagacg tacctgccgg
     1621 gcacgctctc gcccgccgtg ctgtcgggcc tgctgcgccg cgacctcggc ttcgacggca
     1681 tcgtcgtcac cgacgctttg gacatgagcg gactcaccat ctacttcaaa caggacgagg
     1741 ccgccgtgcg cgccgtgctc gcgggcgcag acatgctgct gaagcccgcc gaccctgacg
     1801 ccgtcgcgcg gggcttgcgc gaagcggtcg cctcgggccg tctcgccgag aagcgtatcg
     1861 aggaatcggc gcgcaagatt ctcgcggcca agtacgacct cggcctcgtc aagcagcgca
     1921 ccgcgccgct cgaactgctc gaccaactgg tgtcgagtcc cgaaacgctg aagctcgccg
     1981 acgacgtggc gcgcggcgcc gtcacgctcg tccgcgacga cgcgcgtctc ttgcagcgca
     2041 cggacggcgc gggcctccgc cccggcgcgc gcctcttcaa cctcgccatc acgaacggcg
     2101 aagaccgctt cttcatcgcc aacgccttcg ccggccagct cgcacgctcc gggcggcgcg
     2161 ccgaaacggt cgtgctcgac ggtcgctcga ccgaggaaga ggtgaaggcc gccatcgaga
     2221 aggcgcagaa ggccgatgtc gtcatcgcct cgatgtacgg gcgcgtgcgc tcgggcgcgg
     2281 ccaacagcgt cggtctgccc gaacagggcg cgcgcgtgct ctcgacgctg ctcgaacgca
     2341 aggtgcccgt cgtcggcgtc agcttcggca acccgtacct gctccaaagt ttccccgcgc
     2401 tccagaccta cgtcgtcgcc tacggcgaca tgccgagcct ccaacgcgcc gtcgcccgcg
     2461 ccctgctcgg gcaggcggac atcacgggcc gcctccccat ctcgctcccc ggactctacc
     2521 cgcgcggcac gggcatccag ttgaaagcct cggccacgcc cgcgacggcg atcaagtaaa
     2581 tgagaacaga cgcaagaaaa atcctacgcc gcacgagaag cgccgcgcga agcaccggcg
     2641 atgcgctcca cgcacgacgg tcgcgctccg tcgccgcgtt gcttttgatt tgtctgctga
     2701 cagcacaggc gtccgcgacc acgctgaagg tcgtgccgcc cgaagcggtc gggatgtccg
     2761 cgaagcatct cgcgcacatc gacggggcgg tcgccgggtc aatcgagcgc ggcgagacgc
     2821 cgggcgcggt ggtgctggtg gcgcggcgcg ggggcgtcgt gtggcgcaaa gcctacggcg
     2881 cgcgcgccgt ggtgccgcgg cgcgaggaga tgaccgtgga cacggtcttc gac
//
LOCUS       DBNGOH010000424         1534 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3730772, whole genome shotgun sequence.
ACCESSION   DBNGOH010000424 DBNGOH010000000
VERSION     DBNGOH010000424.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1534)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1534)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1534
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3730772"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            145..756
                     /locus_tag="AC159P_08345"
     CDS             145..756
                     /locus_tag="AC159P_08345"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR3695501.1"
                     /translation="MTSESELIRRAQAGEREAFCQLAKNYERRIYALALHYCRRAEDA
                     EDLSQEVWLKAFRAIGGFRAESGFYTWLRQITVNTFLNGRRAETLTRDEVRTRVRLDS
                     LSEMLDEAAMVPEQSRRDSENGFHRRLMVGRVMRALGELTPRQRLVFLLKHREGMTCA
                     EIAEAVGISVGSVKKSLFRAVQKLRAEMGVGDACVGVGVGEDG"
     gene            773..>1534
                     /locus_tag="AC159P_08350"
     CDS             773..>1534
                     /locus_tag="AC159P_08350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695502.1"
                     /translation="MHDCDVTREMLADLLFDELEEGPRLSLLEDVRICGGCSEQYRAL
                     AETLRVMDVGMDACLPAEEFWPGYEERLSERMAAEIQLSFWQQAAPRGEYSPTILEDS
                     GVARRLLKELRAAACESQLTWPEFKRDPVGFAGRSVSAYTQVAWRFISERNVALATVS
                     SFVLVFFIVGGVYALEQLRLANAVPEASAASDDDEFAVWVNNTNIPRAEEEREDGTPG
                     LSRGKGGGSKPVQERPGGGGGGGDRSENPTSIGKVA"
BASE COUNT          268 a          452 c          564 g          250 t
ORIGIN      
        1 ttctcccttt tcgcctcttc ccccttttcg cccccccctc acggtcggga ttctgccgct
       61 cagaaaaaat accacggggc cgcgccaacc aaactctcgg aagtcgtgtc tatagagtca
      121 accttgcgcg aagggaaacg agagttgacg agcgagagtg aactgatccg gcgggcgcag
      181 gcaggcgagc gcgaagcctt ctgtcagctt gcgaagaact acgagcgccg gatttacgcg
      241 ctggcgctgc actactgccg ccgcgcggag gacgccgaag acctctcgca ggaggtgtgg
      301 ctgaaggctt tccgcgccat cggcggtttc cgcgccgagt cgggcttcta cacatggctg
      361 cggcagatca ccgtcaacac gttcctcaac ggcaggcgcg cggagacgct gacgcgcgac
      421 gaggtgcgaa cgcgggtcag gctcgacagc ctgtccgaga tgttggacga ggcggcgatg
      481 gtgcccgagc agagccggcg tgattcggag aacggctttc accggcggct gatggtcggg
      541 cgcgtgatgc gcgcgctcgg cgagctgacg ccacggcagc ggctcgtctt cctcttgaag
      601 caccgcgagg ggatgacgtg cgcggagatt gcggaggcgg tcggcatctc cgtggggagc
      661 gtcaagaagt cgctcttccg ggcggtgcag aagctgcgcg ctgagatggg cgtcggcgac
      721 gcctgcgtcg gtgtcggtgt gggcgaggat ggatgaagga cggttgagga agatgcacga
      781 ttgcgatgtg acgagagaga tgctggccga cctgctcttc gacgagctgg aagaggggcc
      841 gcgcctcagt ctcctcgaag acgttcgaat ctgcggcggc tgctccgagc agtaccgcgc
      901 gctggcggag acgctccgcg tgatggatgt tggcatggac gcctgtctcc cggcagaaga
      961 gttctggccc ggttacgaag aaaggttgag tgaacgcatg gcggcggaaa tccaactgag
     1021 cttctggcaa caggccgcgc cccgcggcga atacagtccg acgatactgg aagacagcgg
     1081 cgtggcgcgc cggctcttga aggagctgcg cgcggcggcg tgcgaatcgc aactgacgtg
     1141 gcccgagttc aagcgcgacc ccgtgggctt tgcgggccgc tcggtgtcgg cctacacgca
     1201 ggtcgcgtgg cgcttcatct cggagcgcaa cgtggcgctg gcgaccgtct cttcgttcgt
     1261 gcttgtcttc ttcatcgtcg gcggcgtcta cgcgctcgaa caactgcggc tggcgaacgc
     1321 ggtgcccgaa gcgagtgcgg cgagcgacga tgacgagttc gccgtgtggg tcaacaacac
     1381 gaacatcccg cgcgcggagg aggagcgcga ggatgggacg cccggactga gccgcggcaa
     1441 agggggcggc tcgaagcccg tgcaggagcg gcccggcggc ggcggcggcg ggggcgaccg
     1501 gagtgagaac ccgacctcga taggtaaggt ggcg
//
LOCUS       DBNGOH010000425        14898 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_5013649, whole genome shotgun sequence.
ACCESSION   DBNGOH010000425 DBNGOH010000000
VERSION     DBNGOH010000425.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 14898)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14898)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14898
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_5013649"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            362..811
                     /locus_tag="AC159P_08355"
     CDS             362..811
                     /locus_tag="AC159P_08355"
                     /inference="COORDINATES: protein motif:HMM:NF012618.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HIR3695503.1"
                     /translation="MGHTLKGRHSAIVSIDFSPDGQTLMAGATDVRLWDVKTGELLHV
                     LEGYEKYDVRVYGIAWSPDGRTFASTGKGAILLWDAETWQLRRTLEWDTAGQPPTNVV
                     WSPDGREGRVLHSESRRDDLHLGNRVGTAAHNVKRRSTSGWGCGVVA"
     gene            837..1691
                     /locus_tag="AC159P_08360"
                     /pseudo
     CDS             837..1691
                     /locus_tag="AC159P_08360"
                     /inference="COORDINATES: protein motif:HMM:NF012300.7"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
     gene            1698..3293
                     /locus_tag="AC159P_08365"
     CDS             1698..3293
                     /locus_tag="AC159P_08365"
                     /inference="COORDINATES: protein motif:HMM:NF025056.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HIR3695504.1"
                     /translation="MPATVTTKTFKCVKEFVLGLKEEGGRKGVLVSPEELRGRLEALD
                     AEWEFADAEMMTAVRHLANHGYVSVLRGSSGEETILLAPDLLVNLAASFVLEARRSPR
                     GLGALDEARALAGEYEFPELGGLEERERDVLLDAAVVLFIEHNLCFRETLGEQTFLIF
                     PALINQKRPAQEGVETVDDVSYRVSGAVENVYAALVVQLGYTRNFTRTNQWQNQAEYE
                     TARGDVCGFRQVDEREGETELVLYYAKEKRGAQLLFQGLFEEFLRGREVTVTKFPPVS
                     CPKCQYRQQRGEVLKRIGEGKGFLFCGDCGKKIVLPKEGEKLVLSRAEREQLYDEQGG
                     ARRRTQFESALVRVKAVVRDEKKKAPSCFVSYAWGEAGRERWVRGLVTDLQNAGIETV
                     FDQRDNPEFGKSVARFISRITESDFVAVVGTPLYRLKYENKVSTTGSVVAAEVDLINL
                     RLMATEAEKETVLPLLVEGEERVSLPSLMRGRVYGDFLDERFYFATLFFDLILILYRI
                     PFDHPAVSDLRGALRGTDVPRLR"
     gene            3405..4436
                     /locus_tag="AC159P_08370"
     CDS             3405..4436
                     /locus_tag="AC159P_08370"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HIR3695505.1"
                     /translation="MKFNPQNILVIDFGQLGDVVLSLPALGAVRRRFPRARVTVAVGR
                     AAGAVLELAGVADEVLPVDRVGLRDGPKVLSLVRIGQLVGEVRRRKFDFVVDLHSLSE
                     TNLLGFLSGAPRRLFARRPGRSLDYLSNFRPQAPVEDRKKHAIDRYLDVLRPLGVGEV
                     SRVPRLPVRVEDDRAVDEILRKMKAPVTEGPLVGMFPGAGHASRRWPVERFAELAWML
                     ERNDAVRVVLFAGPEERALVKRARGAFPASTVVLDKLSVPQLAAAAARLSVFVSNDTG
                     PMHLAAAVGTPVVILMQHHPMFDCYVPPGERHRVVHARAIEEITPEQAYEAARAALNT
                     ERAASLFSS"
     gene            4497..6161
                     /gene="dacB"
                     /locus_tag="AC159P_08375"
     CDS             4497..6161
                     /gene="dacB"
                     /locus_tag="AC159P_08375"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine endopeptidase"
                     /protein_id="HIR3695506.1"
                     /translation="MNSTPERRRALVAGGASVAVLLALALALPAYVLRSHSQAGGAGA
                     AIDEGAFTSARQSPPRTGRETRARDEELGRRIGESIEKSEAGSARWGVHVVSLRDARV
                     LYERDARRLFAPASNMKLYTTAVALELLGAGHRWRTSVYAAAAPDAAGAIKGDLVLYG
                     RGAPDLSSRAAPGRGSQLSELADKLYARGVRRVRGDVVGDESFFRGEALGAGWLWEDV
                     QWYYGAEPSALSIDDNEVALVVAPGKEVGAAAEVESLPALDAVSVPNELKTIERGARG
                     SFGVTRELSSNAVRVWGELPAGGSSYKVRLSVRRPALVAAALFRDALVARGIEVAGTA
                     RVVDAHTPSGARLDTAFAVELASVTSATLGEVVRETNKKSLNLQAELLLRTLGRERGD
                     TAPDPDAERMRRRDTDAAGLAVVRRWLESAAGAEPGALDLRDGSGLSRLNRVTPEATT
                     RLLLRMSRSPAAAPFRASLPVAGRDGTLERRLRRSNAAGRVAAKTGSFTHVSALSGYA
                     LTADGEPLAFSIICNDDTGGGSERVIDSIVTLLSAYPDVEKSASSR"
     gene            6574..7797
                     /locus_tag="AC159P_08380"
     CDS             6574..7797
                     /locus_tag="AC159P_08380"
                     /inference="COORDINATES: protein motif:HMM:NF015506.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HIR3695507.1"
                     /translation="MNLTLPVILAVAASGLAAHGQALTPSSVGGHHYSYVTAFVQQGN
                     DREQDGLQGPVRRVKTETAKITVKNGKPLEGPRVLLETTTYDNKGTKVDNAYFLAAGG
                     SLTGKEVYRYDDKGNIVEMTLTNEDGTLQSKELYIYEFDAVGNWTKMTTNVAMIEGGK
                     LSYEPTEVTYRTIAYFLDEAMVAKMSQPAVQPAAPTSAPAPATSAPATSAPAASTPAA
                     ANAGANSAATNANAGAQPSKSPAATNPAPAAVAKKENTVSAALPLNIASTDKPAAAVA
                     PAGLTPNTNTVGAGPVVKVEGEAPADATPHPARSGPLKPVSGGILNGKALKMPMPMYP
                     ELARRARLSGMVDVEVVIDMTGKVISAKAVKGPALLHGAAEQAAKNARFSPTLLSGQP
                     VRVTGTISYNFNLSQ"
     gene            7825..8862
                     /locus_tag="AC159P_08385"
     CDS             7825..8862
                     /locus_tag="AC159P_08385"
                     /inference="COORDINATES: protein motif:HMM:NF025690.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HIR3695508.1"
                     /translation="MALTGQLSDMSLAELIEFFCNQRKTGRLKIDYQRGHSVFFIKDG
                     ELVDAKVGTLSGAEAVYYSLTLPNAAFDFSPEVLPTRRSINEPWTQVVLEGLRRLDEG
                     LSPSDADAFGNWTPSEADVAQMMDHVERLGEANSAKEAGQKSEASDAADVPFSFMVDS
                     AAASGGSRKKLMVMGVAASVVLICAVAAVPLMRGSGGKPAESAAAPAPAAQPAAPAEA
                     APAEAAANPNAEAAAPAEAGGAEAEAAEAARREREERERRDRKRRAEEAARKDEAAKA
                     ALTPAPVAAGPKSVRVTVNYDENGRVTQASVAGTSAGAEAYGGTAVRIARGKRFPAGK
                     AGSTVVTIPVN"
     gene            complement(8965..10197)
                     /locus_tag="AC159P_08390"
     CDS             complement(8965..10197)
                     /locus_tag="AC159P_08390"
                     /inference="COORDINATES: protein motif:HMM:NF013696.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HIR3695509.1"
                     /translation="MIVPATTSTPPAVEVVRDLMASERVRRAFVFFETEAERVTAEHV
                     AMCSIPAAPFGEAERAEYLRAKFREFGLAEARVDEEGNSVALRRGRELHPLLVVSAHL
                     DTVFPAGTDYTVRREGARLRAPGIADDGCGLAALMALARAFAECGIETEGSVLFVGTV
                     GEEGEGNLRGVRHLFSAGEWAGKVDAFVSLDGPGVERITNAALGSRRYRVTMRGTGGH
                     SWGDFGVANPVHAMGRAIARLSAYPAPARPRTTFNVGRIAGGAGVNVIPREAAMDVDL
                     RSESAAELSRLDAVFRRAVREAADDENAARRTGTPPLELEVKLIGDRPSGETARENHL
                     VRLAEEATRALGFAPRLDCSSTDSNIPISLGVAAVTLGAGGQSGHSHTLDEWYDPRAR
                     DLGLKRALLVALGVVGLA"
     gene            complement(10298..11521)
                     /locus_tag="AC159P_08395"
     CDS             complement(10298..11521)
                     /locus_tag="AC159P_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011551401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LeuA family protein"
                     /protein_id="HIR3695510.1"
                     /translation="MPASELIYDWNTVEPSAEKPARRLSFDDETLRDGLQSPSVSEPS
                     VERKIELLHLMDALGIDTADIGLPGAGGTHAAGVERLAREIVEQKMRIRPNCAARTHR
                     NDIRPIAEISQRAGIPIEACTVLGSSFIRVYAEDWTLDKLLKLTEEAVTFAVSEGLPV
                     MFVTEDTTRAEPETIRALYTTAIRSGARAVCVCDTVGHATPDGARAVVRFVRKLIDEE
                     GGGIRLDWHGHQDRGLGVINSIAAIEGGADQVHGSALGVGERVGNTPMDQLLVNLKLM
                     GWIDNDLARLREYCDKAAEACEWTIPLNYPVFGRDAFRTATGVHAAAVIKSFRKGDNE
                     LADLIYSGVPAGQFGMRQRIDIGPMSGKSNVVYWLESRGLEPTDERVQRIYDHAKGAH
                     GVLSDEEIMQVLRTE"
     gene            11748..12332
                     /locus_tag="AC159P_08400"
     CDS             11748..12332
                     /locus_tag="AC159P_08400"
                     /inference="COORDINATES: protein motif:HMM:NF014041.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF84 family protein"
                     /protein_id="HIR3695511.1"
                     /translation="MPLNSVRIALGSSRASKIMAVRAASARVAAADARWARAEIITRA
                     IETSVPAMPLTDSHLMRGARERADGVRELLSSEGQRADYYVGLEGGFHSITMDGRRHT
                     FLRGWAYAADEAGRGHYGAAPSVTVPPSIVARVERTGRELSEVMDEVAGEYDVRSRQG
                     AWGVLSRGLLTRAMSFETALIAAFAPFYNARLYE"
     gene            12381..12998
                     /locus_tag="AC159P_08405"
     CDS             12381..12998
                     /locus_tag="AC159P_08405"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HIR3695512.1"
                     /translation="MTADGSDEHLAKIGHGATGWIEVIAGSMFSGKSEELIRRLRRAR
                     IARLKVQVFKPEIDVRFSKDHIVSHSEMRHESVPVKTAADILARVEPGTEVVGIDEGQ
                     FFDNELVHVVNTLADRGVRVIIAGLDQDYTGKPFDPMPQLLAVAEYITKTHAICVRCG
                     QPANYSQRTVNVEGQVVVGASDAYEARCRACFVPHADTPTRHDTE"
     gene            13188..14069
                     /locus_tag="AC159P_08410"
     CDS             13188..14069
                     /locus_tag="AC159P_08410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695513.1"
                     /translation="MLTALANAACSEATVTNANTTTTNATTNINSPSNANMTVTTTKT
                     GAGTVIESKEPDKYSATMVLTAATSGQQPQAGKTEIKVARNGSDRRYSIDTRIPTVGE
                     IIFLDKADKRYLILPARKQYAELTADLTGFDVGRAMTPGQLVAYVSRQQGVELVGEET
                     LNNRPAMKYRYAGKTQTSSTAGEVAGETYIYVDKETGLPLRVEGFGQSSGNVQGVSGG
                     RATLETRDLKTEVNPADFELPSGFSQITAEQLRQQTAQLMQFVQGIMGLINAQAQAVA
                     TPAAATATPAAATSPAR"
     gene            14212..>14898
                     /locus_tag="AC159P_08415"
     CDS             14212..>14898
                     /locus_tag="AC159P_08415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695514.1"
                     /translation="MRYLLTLVLGVLVGGALVYVLFVGAPGSKPMPGSPVAAPDASGA
                     PPGTAVVELGEQFFNTLLGTVFKDLGEPTFRLARAGSRDTEGAGDPLYVPAQGGCQNQ
                     VVVAPEGGGVQTGVLLENNQVLVPLAFKGTYNLFGNCMNFNGTAQANIQLRFAPEEQT
                     LYGQLNVEGVNLAGMSPIFSGPVTLFVQNSLNQRVNPLVLMRGQQISLNIPVQATGGA
                     LKAQAKDIRAE"
BASE COUNT         2458 a         4845 c         5284 g         2311 t
ORIGIN      
        1 gccctgcccc gacggtcttg cgacggtctt tgattctctg cgccgttata ggccacctcc
       61 gcctccttct cgattgagca cgccccccga tgtgctatcc ttcccgcctc aaggcagcac
      121 tctcccgccg gataagccgc cgcccctccg acacggcccc atccgccggg tgaaaagaga
      181 accaacaccg atgcccgacg aaaccacgcc cgaccaaatc ccgcccggct tcaaactcgg
      241 gcacacgctc cgcgatcacg agggaccgat cttccgaatc gcgtgatcgc cggacgggct
      301 gacgctcgcc tccggcgggc acgacgaaat cgcgcggctc tgggacgccg agacggggca
      361 actgggtcac actttgaaag ggcggcatag cgccatcgtc agcatcgact tctcgccgga
      421 cgggcagacg ctcatggcgg gggccacgga cgttcggctg tgggatgtta agaccggcga
      481 actgctccat gtgctcgaag gatatgagaa gtacgacgtt cgggtctacg gcatcgcgtg
      541 gtcgcccgac gggcggacgt tcgcgtccac ggggaagggc gcgattctgc tgtgggatgc
      601 cgagacgtgg caactgcgcc gcacgctgga atgggacacc gcgggccagc cgcctaccaa
      661 cgtggtctgg tcgccggacg ggcgcgaggg tcgcgtcctc cactctgagt cgagacggga
      721 cgatctacat ttgggaaacc gggtcgggac ggctgcgcat aatgtcaaga ggcgctccac
      781 gtcaggatgg ggctgtggcg tggtcgcctg acggtcgcat catcgcgtcc gggggtatgg
      841 agagcgtcgt cctgcgagat gcggcgacgg gtcagccggt gatcgtcctc gaggggcata
      901 ccgggaacgt caagtccgtg agcttctctt tcgacggctc gctgcttgcc tcgaaggcag
      961 gcgacgacac cgtcaggctg tggcgctgcg atacgtggca gaacgtcgcc acgctcgccg
     1021 aagcggcttc cgactactgg ccgccgagtc tcgccttcca cccaacggcg cccgtgctgg
     1081 cgaccctcgg cgaggaagac acggtcgtcc gcatctggga cttggatttc gagactatcc
     1141 tcggagccgc gcccgagcgg gaggacgttt tatacacgac ggcgaagatt acgctggtgg
     1201 gtgacagcgg cgtggggaag acggggctgg gttggcggtt ggcacatagg gagtttaggg
     1261 agcattcttc gacgcacggg cagcaggtct gggtggttga tgagttcggg gcgataagga
     1321 gggatgggac gcagtgcgag gcggtgttgt gggatttggc ggggcagccg gactagcgtc
     1381 tggtgcatgc gttgtttttg gatgacgttg atgtggcgtt ggtgctgttc gacccgacga
     1441 acaggcagga gcctttgaag ggggtggagt actggttgaa gcagcttgcg cgggcgaggc
     1501 gggacgggcg gaagcggcgg gtgattgtgg tcggggcgcg ggcggatagg ggcgtgccga
     1561 cgttgacgcg ggaggagttg gaggagttct gcaagcggca tggcgtggag ggcgggtacg
     1621 tcgggacgag cgcgttgacg ggagaggggc ttgaggagtt ggtggagcgt ttgaaggcgt
     1681 cgattgtgtg agaggagatg cccgcgacgg tgacgacgaa gacgttcaag tgcgtgaagg
     1741 agttcgtgct ggggttgaag gaggagggcg ggcgcaaagg ggtgctggtc agccccgaag
     1801 agttgcgcgg gcggctggaa gcgctggatg ccgagtggga gttcgcggac gcggagatga
     1861 tgacggcggt gcgccacctc gcgaatcacg ggtacgtgtc ggtgctgcgc ggctcgtcgg
     1921 gcgaggagac catcctgctc gcgccggact tgctcgtgaa tctggcggcg tcgttcgtgt
     1981 tggaagcgcg gcgcagcccg cgcggattgg gtgcgctgga cgaggcgcgc gcgctcgcgg
     2041 gcgagtacga gtttccggag cttggcggtt tggaagagcg ggagcgggac gtgctgctcg
     2101 acgcggcggt ggtgctgttc atcgagcata acctgtgctt ccgcgagacg ctgggcgagc
     2161 agacgtttct gatcttcccg gcgttgatta accagaagcg gcccgcgcag gaaggggtcg
     2221 agacggtgga cgacgtttct tatcgcgtga gcggcgcggt cgagaacgtg tacgcggcgc
     2281 tggtggtgca gttgggctac acgcggaact tcacgcggac gaaccagtgg cagaatcagg
     2341 cggagtacga gacggcgcgg ggcgacgtgt gcgggttccg gcaggtggac gagcgcgagg
     2401 gcgagacgga actggtgctc tattacgcga aggagaagcg gggggcgcag ttgctctttc
     2461 aggggctgtt cgaggagttc ctgcgcgggc gcgaggtgac ggtgacgaag ttcccgcccg
     2521 tgtcgtgccc gaagtgtcag tatcgtcagc agcgcggcga ggtcctcaag cgcatcggcg
     2581 agggcaaggg ctttctcttc tgcggcgact gcggcaagaa gatcgtgctg ccgaaggaag
     2641 gcgagaagct cgtgctgagt cgcgccgagc gcgagcagtt gtacgacgag caggggggcg
     2701 cgcggcggcg gacgcagttc gagtcggcgc tggtgcgcgt gaaggcggtc gtgcgcgacg
     2761 agaagaagaa ggcgccgagc tgcttcgtca gttacgcgtg gggcgaggcg gggcgcgagc
     2821 gctgggtgcg cggcctcgtg accgacttgc agaacgcggg catcgagacc gtcttcgacc
     2881 aaagggacaa cccggagttc ggcaagagcg tcgcacgctt catcagtcgt atcacggaga
     2941 gcgacttcgt cgcggtcgtc gggacgccgc tgtaccgcct gaagtacgag aacaaggtct
     3001 ccacgaccgg gagcgtcgtc gccgccgagg tcgatttgat caacctccgc ctgatggcga
     3061 cggaggcgga aaaggagacg gtgctgccgc tgctcgtcga aggcgaggag cgcgtgtcgc
     3121 tgccgtcgtt gatgcggggg agggtgtacg gagactttct ggacgagaga ttctatttcg
     3181 ccacgctctt cttcgacctg atcctgatac tctaccgcat ccccttcgac catccggcgg
     3241 tcagcgactt gcgtggggcg cttcgcggca cggatgtgcc gcggttgagg tagacgcgcg
     3301 cgaggcgcgg gccggggctt aagggcgcgc cgcgcgcgcc gggtcgtgcg ggccgaggct
     3361 tagggcgaaa cgaatcgggg cgcgccgtcg ttatcgagat ggtgatgaaa ttcaatccgc
     3421 agaacattct ggtgatcgat ttcgggcagt tgggcgacgt ggtgctcagt ctgcccgcgc
     3481 tcggcgctgt caggcggagg tttccgcggg cgcgtgtgac ggtggcggtg gggcgcgcgg
     3541 cgggggcggt gttggagttg gcgggggtgg cggacgaggt gttgccggtt gacagggtgg
     3601 ggttgcggga cgggccgaag gtgttgtcgt tggtgcgtat cgggcagttg gtgggggagg
     3661 tgcggcggcg gaagttcgat ttcgtggttg acctgcacag cctgtcggag acgaacctgc
     3721 tggggtttct gtcgggcgcg ccgcggcgtc tgttcgcgcg caggccgggg cgttcgttgg
     3781 attacctgtc gaacttcaga ccgcaggcgc cggtcgagga taggaagaag cacgcgattg
     3841 atcgttatct ggacgtgctc aggccgctgg gggtgggcga ggtgtcgcgt gtgccgcggc
     3901 tgccggtgcg cgtggaggac gaccgcgcgg tggacgagat tctgcggaag atgaaggcgc
     3961 cggtgacgga ggggccgctg gtcgggatgt ttccgggcgc ggggcacgcg agcaggcgtt
     4021 ggccggtgga gcgcttcgcc gagctggcgt ggatgttgga gcggaacgac gcggtgcggg
     4081 tggtgctctt cgcggggccg gaggagcggg cgctcgtcaa gcgcgcgcgg ggggcgttcc
     4141 cggcttcgac cgtcgtgttg gataaactgt ccgtgccgca gctcgcggcg gcggcggcgc
     4201 ggctctcggt cttcgtctcg aacgacacgg ggccgatgca cttggcggcg gcggtcggga
     4261 cgccggtcgt catcctgatg cagcaccacc cgatgttcga ctgctacgtg ccgcccggcg
     4321 agcgacaccg cgtcgtccac gcgcgcgcca tcgaagagat caccccggag caggcttacg
     4381 aggcggcgcg cgccgcgctg aacacggaac gcgccgcctc gctcttctca agctgaagca
     4441 aaaatactct cccacatcgg gttgaaaaat ttctccctgt ccaggtcgat gaagtcatga
     4501 acagcacccc cgagaggcgg cgtgctctcg tcgcgggcgg cgcttcggtg gccgtccttc
     4561 tcgcgttggc gctcgcgctg ccggcgtacg tcctgcgctc gcactcgcag gcgggcggcg
     4621 cgggcgcggc cattgacgaa ggggcgttca caagcgcccg acaatcgccg ccgcgcacgg
     4681 gccgcgagac gcgcgcgcgg gacgaagaac tcggtcggcg aatcggggag tcgatcgaaa
     4741 agagtgaagc gggttcggcg cgctggggcg tccacgtcgt ctctctgcgc gacgcgcgcg
     4801 tgctctacga gcgcgacgcg cggcgcctgt tcgcgcccgc ctccaacatg aagctctaca
     4861 cgacggcggt cgcgctcgaa ctgctcggcg cagggcaccg ctggcgcacg tcggtctacg
     4921 ccgccgccgc gcccgacgcg gcgggcgcga tcaagggcga cctcgtgctc tacggtcgcg
     4981 gcgcccccga cctctcttcg cgcgccgcgc cgggcagggg gtcgcaactc tccgagctgg
     5041 ccgacaagct gtacgcgcgc ggcgtgcggc gcgtacgggg agatgtcgtg ggcgacgaga
     5101 gcttcttccg cggcgaggcg ctcggcgcgg ggtggctgtg ggaggacgtg cagtggtact
     5161 acggcgcgga gccgagcgcg ctctcgatag acgacaacga ggttgcgctc gtcgtcgcgc
     5221 ccggcaaaga ggtcggggcg gctgccgagg tggagtcgct ccctgcgctc gacgcggtta
     5281 gcgtcccgaa cgagttgaag accatcgagc gcggcgcgcg cggctccttc ggcgtcacgc
     5341 gcgagctgtc ttcgaacgcg gtgcgcgtgt ggggcgagtt gcccgcgggc ggcagcagct
     5401 acaaagtgcg cctctcggtt cggcgccccg cgctcgtcgc ggcggcgctc ttccgcgacg
     5461 ccctcgtcgc gcgcggcatc gaggtcgcgg gcacggcgcg ggtcgtggac gcgcacacgc
     5521 cttcgggcgc gaggctcgac acggcgttcg ccgtcgagct tgcctcggtc acgagcgcga
     5581 cgctcggaga ggtcgtacgc gagaccaaca agaagagcct gaacctgcaa gcggagctgc
     5641 tgctgcgcac gctcggcaga gagcgcggcg acaccgcgcc cgacccggac gccgaaagga
     5701 tgcgccgccg cgatacggac gccgcgggcc tcgccgtcgt ccgccgttgg ctcgaaagcg
     5761 cggcgggcgc cgaaccgggc gcgctcgacc tgcgcgacgg ctccggcctc tcgcgcctca
     5821 accgcgtcac gcccgaagcg accacgcgcc tgctcttacg aatgtcgcgc tcgcccgccg
     5881 ccgcgccctt ccgcgcctcg ctccccgtcg ccgggcgcga cggcacgctc gaacgccgcc
     5941 tccggcgctc gaacgcagcg ggccgcgtcg ccgccaagac cggctcattc acacacgtca
     6001 gcgcgctctc cggctacgcc ctcaccgccg acggcgaacc gctcgccttc tccatcatct
     6061 gcaacgacga cacaggcgga ggctccgaac gtgtcatcga ttccatcgtg acgctgctct
     6121 ccgcctatcc ggacgtggaa aaatccgcct cttcgagata agaacacaaa attaccggtt
     6181 ggcagatgtt tgtgatggac aatcagtgtt taatatgtat aatgtctgtg ccgccacgta
     6241 aaaaaagtgg cgggcgggtg aaacgaatct cccatcgagt gcggagcgcg cgagtctgaa
     6301 gcgaatgtaa gctcttacgc gagcactact tacccgtgat actcacgcct cgcgccgcgg
     6361 gccgcgaacg actcctggtc gtgccgccgt tcggccccgg cgaacgaact taacaaactc
     6421 gccccggcag cgtccttcct aaattcatgc cgccggtgga gtgactttgg atttcgggac
     6481 ggccacccag ccgcgcccgt gaacggaagc taacccctgc ccgagcatcc cttgcttcac
     6541 gaaacttcag gagtaactgt catgtcgaga cgaatgaatc tgacccttcc ggtgattctt
     6601 gccgtcgccg cctccggcct cgctgcccac gggcaagcgc tgacgccgtc gtcggtcggc
     6661 ggtcatcact actcgtacgt caccgcgttt gtccagcagg ggaatgaccg cgagcaggac
     6721 ggcctacagg ggcctgtgcg ccgcgtgaag accgagacgg ccaagatcac ggtgaagaac
     6781 gggaagcccc tggaggggcc gcgcgtcctg ctggagacga cgacctacga caacaagggg
     6841 acgaaggtgg acaacgccta cttcctcgcg gcgggtgggt ctctgacggg caaggaggtc
     6901 tacaggtacg acgacaaggg caacatcgtc gagatgacgc tgacgaacga ggacggcacg
     6961 ctgcaatcca aggagcttta catctacgag ttcgacgcgg tcggcaactg gacgaagatg
     7021 acgaccaacg tcgccatgat cgagggcggc aaactcagct acgagccgac cgaagtcacc
     7081 taccgcacca tcgcctactt cctcgacgag gcgatggtcg ccaagatgtc gcagcccgcc
     7141 gtgcagcccg ccgcgccgac ctcggcgccc gcgcccgcga cctcggcgcc cgcgacttcg
     7201 gcgcccgccg cttcaactcc cgccgccgcg aacgcgggcg cgaactccgc cgcgacgaac
     7261 gcgaacgcgg gcgcgcagcc gagcaagtcg cccgcggcga cgaacccggc cccggcggcg
     7321 gtcgcgaaga aagagaacac cgtctctgcc gccctgccgc tcaacatcgc ctcgacggac
     7381 aagcccgccg cggcggtcgc gcccgcgggc ctgacgccga acacgaacac ggtcggcgcc
     7441 ggccccgtcg tgaaggtcga gggcgaggcc cccgccgacg cgacgccgca cccggcgcgc
     7501 agcggcccgc tcaagccggt ctcgggcggc atcctcaacg gcaaggctct gaagatgccg
     7561 atgcccatgt accccgaact cgcgcggcgc gcgcgcctga gcggcatggt ggatgtcgag
     7621 gtggtgatcg acatgacggg caaggtgatt tcggcgaagg ccgtcaaagg cccggcgctg
     7681 ctgcacgggg cggcggagca ggccgcgaag aacgcgaggt tctcgcccac gctcctctcg
     7741 ggccagcccg tccgtgtcac cggcacgatc tcctacaact tcaacctcag ccagtaacct
     7801 gaatagatta agaggaacat tctcatggca ctgaccggac agttgagcga catgtcgctg
     7861 gcggagttga tcgagttctt ctgcaaccag cgcaagaccg ggcgcctcaa gatcgactac
     7921 cagcgcgggc acagcgtctt cttcatcaag gacggcgagc tggtggatgc gaaggtcggc
     7981 accctctccg gcgccgaggc cgtctactac tccctgacgc tgccgaacgc ggccttcgac
     8041 ttcagccccg aagtgctgcc cacgcgccgc tcgatcaacg agccgtggac gcaggtcgtg
     8101 ctcgaaggcc tgcgccgcct cgacgaggga ctctcgccgt cggacgccga cgccttcggc
     8161 aactggacgc cttcggaggc cgacgtggcg cagatgatgg atcacgtcga gcgactcggc
     8221 gaggccaact cggcgaagga ggcgggccag aagtccgagg cgagcgacgc ggctgacgtg
     8281 ccgttctcgt tcatggtcga tagcgccgcg gcgagcggcg gcagtcgcaa gaagttgatg
     8341 gtgatgggcg tcgcggcgag cgtcgtcctc atctgcgcgg tcgccgccgt gccgctcatg
     8401 cgcggctcgg gcggcaagcc cgcggagagc gccgccgcgc cggccccggc ggcgcagccc
     8461 gccgcaccgg cggaagccgc gcccgcagag gccgcggcca acccgaacgc cgaagcggca
     8521 gcgccggcgg aagcgggcgg agccgaagcc gaagccgcag aggccgcgcg ccgcgagcgc
     8581 gaagagcgcg agcgccgcga ccgcaagcgc agggccgaag aggcggcgag gaaggatgag
     8641 gcggcgaagg ccgcgctgac ccccgcgccg gtcgcggcgg ggccgaagtc cgtgagggtc
     8701 acggtgaact acgacgaaaa cgggcgcgtc acgcaggctt cggtcgcggg cacctccgcc
     8761 ggcgccgaag cctacggcgg caccgccgta cgcatcgcgc gcggcaagcg cttccccgcc
     8821 ggcaaggccg gctccacggt cgtcaccatc ccggtcaact aatcaaggcg aaaaggggaa
     8881 gaggggaaaa ggcgaaaagg cgaaaagggg gaagattgtc ttctcccttt tcgccttttc
     8941 gcctcttccc ctcttcccct tcacttatgc cagcccgacg acgccgagcg cgacgagcag
     9001 agcgcgcttg agtccgaggt cgcgcgcgcg cgggtcgtac cactcgtcga gcgtgtgcga
     9061 gtggccgctc tggccgcccg cgccgagggt gacggcggcg acgccgagcg agatcgggat
     9121 gttcgagtcg gtcgaggagc aatcgaggcg cggcgcgaag ccgagcgcgc gcgtcgcctc
     9181 ttcggcgagg cggacgaggt ggttctcgcg cgcggtctcg ccgctcgggc ggtcgccgat
     9241 cagcttcact tccagttcga gcggcggcgt ccccgtgcgg cgcgcggcgt tctcgtcgtc
     9301 ggcggcctcg cgcacggcgc ggcggaagac ggcgtcgagg cgcgagagtt ccgccgccga
     9361 ctccgagcgc aggtcaacgt ccatcgcggc ctcgcggggg atgacgttga cgcccgcgcc
     9421 gcccgcgatg cgcccgacgt tgaaggtcgt gcgcgggcgc gcgggcgccg ggtaggccga
     9481 gaggcgggcg atggcgcggc ccatcgcgtg gacggggttg gccacgccga agtcgcccca
     9541 cgaatggccg cccgtgccgc gcatcgtcac gcggtagcgg cgcgagccga gcgccgcgtt
     9601 cgtgatgcgc tcgacgccgg ggccgtcgag gctgacgaag gcatcgacct tgcccgccca
     9661 ctcgcccgcg ctgaaaagat ggcgcacgcc gcgcaagttc ccttcgccct cttcgcccac
     9721 cgtcccgacg aacagcacgg aaccttcggt ctcgatcccg cactcggcga acgcgcgcgc
     9781 cagcgccatc aacgccgcca gcccgcaccc gtcgtccgcg atgcccggcg cgcgaaggcg
     9841 tgcgccctcg cgccgcaccg tgtaatcggt gcccgcgggg aacacggtgt cgaggtgcgc
     9901 gctcacgaca agcagcggat gaagctcgcg cccgcgccgc agcgccacag agttcccttc
     9961 ctcatcgacg cgcgcttcgg cgagaccgaa ctccctgaac ttggcgcgca ggtactcggc
    10021 ccgctccgcc tcgccgaacg gcgccgccgg gatcgagcac atcgccacgt gctccgccgt
    10081 cacgcgctcg gcctccgtct cgaagaacac gaacgcgcgc cgcacgcgct ccgacgccat
    10141 caggtcgcgc acgacctcga ccgcgggcgg ggtggacgtg gtagcgggaa cgatcataaa
    10201 aaaagaagtg acgaggactg aggactgtgt gaaaagccgt gaaacgtgaa ccgtgatgag
    10261 tccgaccagc gtcgctctca tcaatcggca atcgctttca ctcagtcctc agtacttgca
    10321 ttatttcctc gtcgctgagg acgccgtggg cgcctttggc gtggtcgtag atgcgctgga
    10381 cgcgttcgtc ggtcggctcc agcccgcgcg attcgagcca gtagacgacg ttggacttgc
    10441 cgctcatggg gccgatgtcg atgcgctggc gcatgccgaa ctggccggcg gggacgcccg
    10501 agtagatgag gtctgcgagt tcgttgtcgc ccttgcggaa gcttttgatg acggcggcgg
    10561 cgtgcacccc ggtcgcggtg cggaaggcgt cgcgcccgaa gacggggtag ttgagcggga
    10621 tcgtccactc gcaagcttcg gcggccttgt cgcaatactc gcgcaggcgc gcgaggtcgt
    10681 tgtcgatcca gcccatcagc ttgaggttga cgagcagttg atccatcggc gtgttgccga
    10741 cgcgctcgcc gacgccgagc gcggagccgt gcacctgatc ggccccgccc tcgatggccg
    10801 cgatggagtt gatgacgccg aggccgcgat cctgatggcc gtgccagtcg aggcggatgc
    10861 cgccgccctc ttcgtcgatg agcttgcgga cgaagcggac gaccgcgcgc gcgccgtccg
    10921 gcgtggcgtg ccccaccgtg tcgcagacgc acacggcgcg cgcgccgctg cgaatggccg
    10981 tcgtgtagag cgcgcggatg gtctccggct cggcgcgcgt cgtgtcctcg gtgacgaaca
    11041 tcacgggcaa gccttccgag acggcgaagg tgacggcctc ttcggtaagc ttcaggagtt
    11101 tgtcgagcgt ccagtcttcg gcgtagacgc ggataaagct tgagccgagt acggtgcacg
    11161 cttcgatggg gatgcccgcg cgctgcgaga tttcggcgat ggggcggatg tcgttccgat
    11221 gcgtgcgcgc ggcgcagttc gggcggatgc gcatcttctg ctcgacgatc tcgcgggcga
    11281 ggcgttcgac cccggcggcg tgcgtgccgc ccgcgcccgg caggccgatg tccgccgtgt
    11341 cgatgccgag cgcgtccatc aggtggagca gctctatctt gcgctcgacc gaaggctccg
    11401 agaccgaagg cgattgcagc ccgtcgcgca gcgtctcgtc gtcgaagctg aggcggcgcg
    11461 cgggcttctc cgcgctcggc tcgaccgtgt tccagtcgta aatcagttca ctcgcgggca
    11521 ctctcggtgg cctcctatgc gtaaggcgaa tccctcgctc tgcggcgtgt gttaaggaga
    11581 atttcgatta cagcaagcgc ccccggcaaa gtcaagcaag gcgggcggcg ggcgctcttt
    11641 gacgcggcgc ggcgcgctgt tatagccttc gggcacgctt cgccttcggc ggcgcgccct
    11701 gcgcgaatcg atggccgagg cgctcccgaa acttcgaacg gcccgcgatg cctctgaact
    11761 ccgtccgtat cgccctcggc tcgtcgcgcg cgtccaagat catggccgtg cgcgcggcct
    11821 cggcgcgcgt ggcggcggcg gacgcgcgct gggcgcgggc cgagatcatc acgcgcgcca
    11881 tcgagacgag cgtgcccgcg atgcccctga ccgacagcca cctgatgcgc ggcgcgcgcg
    11941 agcgggccga cggcgtgcgc gagttgttat cgagcgaggg gcagcgcgcc gactactacg
    12001 tcggcctcga aggcggcttc cactcgatca cgatggacgg caggcgccac accttcctgc
    12061 gcggctgggc ctacgccgcg gacgaagcgg ggcgcggcca ctacggcgcg gcgccctcgg
    12121 tcacggtgcc gccgtcgatc gtcgcgcgcg tcgagcgcac gggccgcgag ctgtcggagg
    12181 tgatggacga ggtcgcgggc gagtacgacg tgcgcagccg gcagggcgcg tggggcgtgc
    12241 tctcgcgcgg gctgctcacg cgtgcgatga gcttcgagac ggcgctcatc gccgccttcg
    12301 cgccgttcta caacgcgcgg ctgtacgaat agagttttct cggaatcgtc aagcgaaagg
    12361 tcagagcgta gatggaaaga atgaccgccg acggcagcga cgagcacctg gcgaagatcg
    12421 ggcacggggc gacggggtgg atcgaggtca tcgccggctc gatgttctcg ggcaagtccg
    12481 aggagctgat ccggcggctg cgccgggcgc gcatcgcgcg gctcaaggtg caggtcttca
    12541 agccggagat cgacgtgcgc ttctcgaagg atcacatcgt ctcgcactcc gagatgcgcc
    12601 acgagtcggt gccggtgaag accgccgccg acatcctcgc gcgcgtcgag ccgggcaccg
    12661 aggtggtcgg catcgacgag gggcagttct tcgacaacga actcgtccac gtcgtcaaca
    12721 cgctcgccga ccgcggcgtg cgcgtcatca tcgccggcct cgaccaggac tacaccggca
    12781 agcccttcga cccgatgccg caactcttag ccgtcgccga gtacatcacc aagacgcacg
    12841 ccatctgcgt ccgctgtggc cagcccgcca actactcgca gcgcaccgtc aacgtcgaag
    12901 ggcaggtcgt cgtcggcgcc tcggacgcct acgaggcccg ctgccgcgcc tgcttcgtcc
    12961 cccacgccga cacgccgacg cgccacgaca ccgaataaaa aggaaagggg aaaagggaag
    13021 aactgttttc gccctttccc cttttcccct tttccccttt ctttgttatc cttctcggca
    13081 gttccatccc ccgaaaatcg aacgttcgac gatctctgcc tttgagggag gagaagcttg
    13141 cgatgtgtct tcggagaact tctaagggtc tattctttgc cgcgctcatg ttgacggccc
    13201 tcgccaacgc ggcgtgcagc gaggcgacgg tgacgaacgc gaacacgacg acgacgaacg
    13261 ccaccaccaa catcaacagc cccagcaacg ccaacatgac cgtgacgacg accaagacgg
    13321 gcgccggcac cgtcatcgag tcgaaggagc cggacaagta cagcgcgacg atggtgctga
    13381 cggcggccac ctcgggccag cagccgcagg ccggcaagac cgagatcaag gtcgcgcgca
    13441 acggctccga ccgccgctac tccatcgaca ccagaatccc gaccgtcggc gaaatcatct
    13501 ttctcgacaa ggccgacaag cgctacctga tcctgcccgc gcgcaagcag tacgccgagt
    13561 tgaccgccga cctcacgggc ttcgacgtgg ggcgcgcgat gacgccgggc cagctcgtcg
    13621 cctacgtctc gcgccagcag ggcgtcgagc tggtcggcga ggagaccttg aacaaccgcc
    13681 ccgccatgaa gtatcgctac gcgggcaaga cgcagaccag ctcgaccgcg ggcgaggtcg
    13741 cgggcgagac ctacatctac gtggacaagg agacgggcct cccgttgcgc gtcgagggct
    13801 tcggccagtc gtcgggcaac gtgcagggcg tcagcggcgg gcgggccacg ctcgaaacgc
    13861 gcgacctcaa gaccgaggtc aaccccgccg acttcgagct gccgtcgggc ttcagccaga
    13921 ttaccgcgga gcaactgcgc cagcagaccg cgcagctcat gcagttcgtg cagggcatca
    13981 tgggtctcat caacgcgcag gcccaggccg tcgccacgcc cgccgccgcc accgccacgc
    14041 ccgccgccgc cacgtcgccg gcaaggtgat aacgatttcg gagttcggag ttcggattgc
    14101 cgagcgaaga gcggatgacg attgatgaca gcggcgctgt catcaatcgt cttcggtctt
    14161 tcaatccgaa atccgcaatc cgcaatccgc aatgtcttca ggggggaacg catgagatac
    14221 ctgctcacac tcgtcctcgg cgtgctggtc ggcggcgcgc tcgtctacgt cctgttcgtc
    14281 ggcgcgccgg gcagcaagcc gatgccgggg tcgcccgtcg ccgcaccgga cgcttcgggc
    14341 gccccgccgg gcacggcggt cgtcgaactc ggcgagcagt tcttcaacac tttgctcggc
    14401 acggtcttca aagacctcgg cgagccgacc ttccgcctcg cccgcgcggg gtcgcgcgac
    14461 accgaaggcg cgggcgaccc gctttacgtc cccgcgcagg gcggttgtca gaatcaggtg
    14521 gtggtggcgc ccgagggcgg cggcgtgcag acgggcgtgc tgcttgagaa caatcaggtg
    14581 ctcgtgccgc tcgccttcaa gggcacctac aacctcttcg gcaactgcat gaacttcaac
    14641 ggcacggcgc aggccaacat ccagctccgc ttcgcgcccg aagagcagac cctctacggc
    14701 cagctcaacg tcgagggcgt caacctcgcc ggcatgtcgc ccatcttcag cggccccgtc
    14761 acactcttcg tacagaactc gctcaaccag cgcgtcaacc ccctcgtcct catgcgcggc
    14821 cagcagatct cgctcaacat ccccgtccag gccaccggcg gcgcgctcaa agcccaggcc
    14881 aaagacatcc gcgccgaa
//
LOCUS       DBNGOH010000426         2790 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4646901, whole genome shotgun sequence.
ACCESSION   DBNGOH010000426 DBNGOH010000000
VERSION     DBNGOH010000426.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2790)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2790)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2790
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4646901"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1628)
                     /gene="sppA"
                     /locus_tag="AC159P_08420"
     CDS             complement(<1..1628)
                     /gene="sppA"
                     /locus_tag="AC159P_08420"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HIR3695515.1"
                     /translation="MAMSRGRKVAVIIVGVVLALVLVGVLVVALFIASVDNEPTVRDN
                     SVLVLKVEGELPDFTNVDPLMGRLFGGNPNSLTSLLTQLKKAKADKRIAAVLLDVGFV
                     ETGWAKAGEVRDAVADFRASGKPIYAYMEVGSDKELYVASAAERVYVAPTGDLFVNGL
                     AAEAMYFRGTFDKLGIYWDSYQIGKYKSSPEHFTRKEMSDGEKEQLNELLDDIFNRYV
                     QTIAEARKKSVEDVRALIDAAPHGARDAERAGLIDKAAYREEVEQELKTRLGYKEDEK
                     LRKVSGHDYRNIRPESLGLNQGEKIAVIFAAGPINLGSSHDGEFGGEQMIGSDTVVKA
                     INDARDDKSVKAIVLRVDSPGGSIYPSDFIWKAVEEAKKKKPVVVSMSDVAASGGYYI
                     SMGAHKIVAEPLTITGSIGIYAYKPVVKEFYDWVGVTSQYLTRGKNAGLFRETERFTD
                     EERQSFENLLKRSYYNEFIPKAAQGRGRDAEYIDSVGQGRVWTGLRAKEKGLVDELGG
                     LDKAVEVAKELAKIPADKGVRRVVYPAPRSFFQQ"
     gene            complement(1820..>2790)
                     /gene="mnmA"
                     /locus_tag="AC159P_08425"
     CDS             complement(1820..>2790)
                     /gene="mnmA"
                     /locus_tag="AC159P_08425"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HIR3695516.1"
                     /translation="PSRCCSLDDVYDARRVAEELGFPFYVLNLERDFERDVVEPFVKS
                     YLEGETPIPCVACNSRLKFASLDRLARSLGCDKVATGHYARVVHDEAAHRYRLLRGRD
                     ARKDQTYFLWELTQEQLAHAMFPLGEMEKAEVREAARRASLYVAEKKESQEICFVPDG
                     DYAGFIDRYLEAEGRADLAPGRGEIVDARGEVLGEHGGIHRYTVGQRRGLGLSRELPL
                     YVVSVEAEANRVVVGHEDELLSTEFEAAGVNWVAFDEPTEAVRADVRVRYRHTEAPAL
                     ITPLGAGRVRVRFDEPQRAVTPGQATVFYRGEEVVGGGWIVKGSRR"
BASE COUNT          437 a          923 c          905 g          525 t
ORIGIN      
        1 aactgctgga agaagctgcg cggcgcggga tagacgacgc ggcggacgcc cttatcggcg
       61 ggaatcttgg cgagttcttt ggcgacctcg accgccttgt ccagcccgcc caactcgtcc
      121 acgagtccct tctccttggc gcgcaggccc gtccagacgc gcccttgccc gaccgagtcg
      181 atgtattcgg cgtcgcgccc gcggccctgc gcggccttcg ggatgaactc gttgtagtag
      241 gaacgcttga gcaggttctc gaagctctgg cgctcctcgt cggtgaagcg ctcggtctcg
      301 cggaagaggc ccgcgttctt gccgcgcgtg aggtactggc tggtgacgcc gacccagtcg
      361 tagaactctt tgacgaccgg cttgtaggcg tagatgccga tggagccggt gatggtcagc
      421 ggctcggcga cgatcttgtg ggcgcccatc gagatgtagt agccgcccga ggcggccacg
      481 tccgacatcg agacgacgac cggctttttc ttcttcgcct cctcgacggc cttccagatg
      541 aagtccgagg ggtagatcga gccgccgggg ctgtccacgc ggagcacgat ggccttgacc
      601 gacttgtcgt cgcgcgcgtc gttgatggcc ttgacgacgg tgtcggagcc gatcatctgc
      661 tcgccgccga actcgccgtc gtgcgaagag ccgaggttga tcgggccggc ggcgaagatg
      721 acggcgatct tctcgccctg gttcagcccg agcgactcgg gcctgatgtt gcggtagtcg
      781 tggcccgaga ccttgcgcag cttctcgtct tccttgtagc cgagccgcgt cttcagctcc
      841 tgctcgacct cctcgcggta ggcggccttg tcgatcagcc cggcgcgctc ggcgtcgcgc
      901 gcgccgtgcg gcgccgcgtc gatgagcgcg cgcacgtctt cgaccgactt cttgcgcgcc
      961 tcggcgatgg tctggacgta gcggttgaag atgtcgtcca gcagctcgtt gagctgctcc
     1021 ttctcgccgt cgctcatctc cttgcgcgtg aagtgctcgg gcgagctttt gtacttgccg
     1081 atctggtacg agtcccagta gatgccgagc ttgtcgaagg tgccgcggaa gtacatcgcc
     1141 tccgccgcca gcccgttgac gaacagatcg cccgtgggcg cgacgtagac gcgctcggcg
     1201 gcgctcgcca cgtaaagctc cttgtccgag ccgacctcca tgtaggcgta gatgggcttg
     1261 cccgaggcgc ggaagtcggc caccgcgtcg cgcacctcgc cggccttcgc ccagcccgtc
     1321 tcgacgaacc ccacgtcgag cagcaccgcc gcgatgcgct tgtcggcctt cgccttcttg
     1381 agctgcgtga gcaggctcgt cagcgagttc gggttgccgc cgaagaggcg gcccatcaac
     1441 ggatcgacgt tcgtgaagtc cggcagctcc ccctcgacct tgaggacgag cacgctgttg
     1501 tcgcggacgg tcggctcgtt atcgaccgag gcgatgaaga gcgccaccac cagcacaccg
     1561 accagcacca gcgccagcac cacgccgacg atgatgacag ctactttcct accacgtgac
     1621 attgccatag cttttacaag aaccctctaa gattgcgttt atcctttgaa ttccgaggag
     1681 accgggccgt tgatattacg aaacggcggg ggaaaagttt aagaagagta ggcagtaggc
     1741 agtaggcagt taatacattt tccgattcga gattcgagat ttggcattgc gagttcggtg
     1801 ttgcttgact gcctactgcc taccgcctac tgcctttcac tatccacccg ccgccgacga
     1861 cctcttcgcc gcggtagaag acggtggctt ggccgggcgt gacggcgcgc tgcggttcgt
     1921 cgaagcggac gcgcacgcgg cccgcgccga ggggcgtgat gagggcgggc gcctctgtgt
     1981 gacggtagcg gacgcgcacg tcggcgcgga cggcttcggt cggctcgtcg aaggcgaccc
     2041 agttgacgcc cgcggcttcg aactccgtcg agagcagctc gtcctcgtgg ccgacgacga
     2101 cgcggttcgc ttccgcctcg acgctgacga cgtagagcgg cagctcgcgc gaaagaccga
     2161 ggccgcgccg ctgcccgacc gtgtagcgat ggatgccgcc gtgctcgccg aggacttcgc
     2221 cgcgcgcgtc cacgatctcg cccctgccgg gggcgaggtc tgccctgcct tcggcttcga
     2281 ggtagcggtc gatgaagccc gcgtagtcgc cgtcgggcac gaagcaaatc tcctgcgact
     2341 ccttcttctc ggcgacgtag aggctagccc tgcgcgccgc ctcgcgcacc tctgccttct
     2401 ccatctcgcc gagcgggaac atcgcgtgcg ccaattgctc ctgtgtcagc tcccacaaaa
     2461 agtatgtctg atccttgcgc gcgtccctgc cgcgcagcag ccggtagcgg tgcgccgcct
     2521 cgtcgtgaac aacgcgcgcg tagtgaccgg tcgccacctt gtcgcagccg aggctgcgcg
     2581 ccagacggtc gagcgaggcg aacttgagac ggctgttgca cgcgacgcag gggatcggcg
     2641 tctcgccttc gagatagctc ttgacgaaag gctcgaccac gtcgcgctcg aagtcgcgtt
     2701 cgaggttgag gacgtagaac gggaagccca gctcttcggc cacgcgccgc gcgtcgtaca
     2761 cgtcgtccag cgaacagcaa cgcgacggca
//
LOCUS       DBNGOH010000427         2077 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2264335, whole genome shotgun sequence.
ACCESSION   DBNGOH010000427 DBNGOH010000000
VERSION     DBNGOH010000427.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2077)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2077)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2077
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2264335"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            102..1856
                     /locus_tag="AC159P_08430"
     CDS             102..1856
                     /locus_tag="AC159P_08430"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011984799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA deacylase
                     activity [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HIR3695517.1"
                     /translation="MRWSHYFIPTLREDPSDAEVVSHRLLLRAGYIRQLGAGIYSLLP
                     LGWRVAQRAMKVIREEMDAIGGQEFFLPALHPAELWKESGRWTAVDETMFRFKDRKGA
                     DMTLGMTHEEVFTAIARHELRSYKQLPQVWYQIQTKFRDEERPKSGLMRLRQFTMKDA
                     YTFDVDAAGLDVSFGHQREAYKKIFDRCGLKYSIVEASSGVMGGSESNEFMARTSAGE
                     DFIAHCAACGYAANLEKATSRLAAVEDEAGLAAPEEFPTPGVRTIEDLTTFPGGAEAS
                     RQIKSLVYVATSATTNEQFIALALLRGDHPLHETKLADSLGASAVRPAQAEEIRELLG
                     ASAGSLGGVGARDKAKAASKQLRVLADESLRGRRNMTTGANKDDHHLRGVDVERDIRP
                     DSWADLRTVAAGERCPRCEEGTLDVFKAMEIGHIFKLGTKYSVSMGATVLTQDGKEVP
                     VVMGSYGIGVERIMSAAVEQSHDADGIVWPRALAPFDVVVTVTNIKQAEMVEAGERLY
                     TDLRRAGLDVLLDDRDERAGVKFKDADLVGIPYRITVGKKIADGKVELFDRAAKQSTD
                     VSIEEVVQLVQRQALAGL"
BASE COUNT          387 a          702 c          668 g          320 t
ORIGIN      
        1 gcttactgct cactgtattt gcttgcgcgc ggcggctgcg cgagaatggg cggacatttc
       61 gcttgagccc gttttagaaa gagagaagaa accgaaagca tatgcgctgg tcacactact
      121 tcatccccac gctccgcgaa gacccttcgg acgcggaggt cgtctcgcat cgcctgctct
      181 tgcgcgcggg ctacatccgc cagctcggcg cgggcatcta ctcgctgctg ccgctcggct
      241 ggcgcgtcgc gcagcgcgcg atgaaggtca tccgcgagga gatggacgcc atcggcgggc
      301 aggagttctt cctcccggcc ttgcacccgg cggagctgtg gaaggagtcc gggcgctgga
      361 cggcggtgga cgagacgatg ttccgcttca aagaccgcaa gggcgccgac atgacgctcg
      421 gcatgacgca cgaggaggtc ttcaccgcca tcgcgcgcca cgagctgcgc tcgtacaagc
      481 aactgccgca ggtctggtat cagatacaga ccaagttccg cgacgaggag cgcccgaagt
      541 cgggcctcat gcgtctgcgc cagttcacca tgaaggacgc ctacacgttc gacgttgacg
      601 cggcgggact cgacgtgtcg ttcggccacc agcgcgaggc gtacaagaaa atcttcgacc
      661 gctgcggcct caagtattcc atcgtcgaag catcctccgg cgtgatgggc ggctccgagt
      721 ccaacgagtt catggcccgg acgagcgcgg gcgaggactt catcgcccac tgcgccgctt
      781 gcggttacgc cgccaacctt gagaaggcca cctcgcgcct cgccgcggtc gaagacgaag
      841 caggtctcgc cgcgcccgaa gagttcccca cgcccggcgt ccgcaccatc gaagacctga
      901 cgaccttccc cggcggcgcc gaagcctcgc gccagatcaa gtcgctcgtc tacgtcgcca
      961 cctcggccac gaccaacgag cagttcatcg ccctcgcgct cctgcgcggc gaccacccac
     1021 tgcacgagac gaagctcgcc gactcgctcg gcgcgtctgc cgtccgcccc gcgcaagccg
     1081 aagagatacg cgagctgctc ggcgcaagcg cgggcagcct cggcggcgtc ggcgcgcgcg
     1141 acaaagcgaa ggcggcgagc aaacagctcc gcgtcctcgc cgacgaatcc ttgcgcgggc
     1201 ggcgcaacat gacgacgggc gcgaacaagg acgaccacca cctgcgcggc gtcgatgtcg
     1261 aacgagacat caggcccgac agttgggcag acctgcgcac ggtcgcggcg ggcgaaaggt
     1321 gcccgcgctg cgaagagggc acgctcgacg tgttcaaggc gatggagatc ggccacatct
     1381 tcaagctcgg gacgaagtat tccgtgtcga tgggcgcgac cgtgttgacg caggacggca
     1441 aggaagtgcc cgtcgtcatg ggcagctacg gcatcggcgt cgagcgcatc atgagtgccg
     1501 ccgtcgagca gagccacgac gccgacggca tcgtctggcc gcgtgccctc gcgcccttcg
     1561 acgtggtcgt caccgtcacc aacatcaaac aggccgagat ggtcgaagcc ggcgagcgcc
     1621 tctacacaga cctccgccgc gccggtctcg acgtgctgct cgacgaccgc gacgagcgcg
     1681 cgggcgtcaa gttcaaggac gccgacctcg tcggcatccc ctaccgcatc accgtcggca
     1741 agaagatcgc cgacggcaag gtcgagctgt tcgaccgcgc cgcgaagcag tcaaccgacg
     1801 tgagcatcga ggaggtcgtc cagctcgtcc agcgacaggc actcgccgga ctctgaccgt
     1861 gggcgtttgc ggttgagcaa ctaacgcctt tctgttcagg ggcggggcgc ccgccgtctt
     1921 aagtcttgaa tcggacttga aacggcgggc gctttcagcc cctacaacca atttcggatg
     1981 gatgtagaac gttcgtaata acggtagggg cagggcttgt ccctgcccgc cgtaacgcgt
     2041 cccttcgacg tcgaagccaa cggggcttga cgggcag
//
LOCUS       DBNGOH010000428         4799 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4097765, whole genome shotgun sequence.
ACCESSION   DBNGOH010000428 DBNGOH010000000
VERSION     DBNGOH010000428.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4799)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4799)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4799
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4097765"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1430)
                     /locus_tag="AC159P_08435"
     CDS             complement(<1..1430)
                     /locus_tag="AC159P_08435"
                     /inference="COORDINATES: protein motif:HMM:TIGR04514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HIR3695518.1"
                     /translation="MHQKQEFTAMARQHWTRSLALALSLLFILQAAPTAWAQNDKNKK
                     KNQDAQDVPDRERTVKKERTDIFKKWMEQDVVYIITSDEKRAFKKLATDEEREQYIEA
                     FWRRRDPNPDTEVNEYLEEHFERIAYANQHFASGIPGWKTDRGRIYIMYGPPNDKESH
                     PMGGQYDRPSYHGGGSTTTYPFEIWFYRHLPGVGSGVEIEFVDPTGTGEYRIARSPNE
                     KDALMHIPGAGLTLGEQLGMSDKADRVAGATGFGYSGPGARVQDSPFEWLQRIVDLQR
                     PPQINEAMEKNLLALVNSPVEDPNSLDVDARVDFFRQSDNSVITAITVQADNRDLKFD
                     DIGGVQTARLNIYGRVTSVAGRRVTSFEDPVVTTATVEELTDTRERKSVYQRVVPLPP
                     GTYKVDLVVRDINSGSQQIKPVGFTVPKYEADKLSASTMVLAVKLQTLKDQLPGMFTI
                     GQHKVIPNVAGVFKRGQDVGIFMQIY"
     gene            complement(1789..2868)
                     /gene="tatC"
                     /locus_tag="AC159P_08440"
     CDS             complement(1789..2868)
                     /gene="tatC"
                     /locus_tag="AC159P_08440"
                     /inference="COORDINATES: protein motif:HMM:NF013097.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HIR3695519.1"
                     /translation="MASSLADTEQDEERDESRLGAQMSFLDHLDELRRRLMRSVAFVF
                     VALVACWFVSDHIYNFLSVPVRRALADAARRPVPLHGLTGGEEILTLDRLQEGDTGRY
                     VFAETTKLGTGVVPVGASVAARVARDRAGELGVFTDEPVFAGATVIPKGVRLPADFKA
                     RAGEGGGAGDERLVVRTAIEPFSLYLQVSLYAAVCLSVPFLLWQVWAFIAPGLYPHER
                     GYALPFIFLSTVSFLIGAAFAYYVLFPPAVTYLLGLGADSFRLFLNATDYFDFIILIM
                     LAMGVVFQMPAVTYVLSRIGIVNAGFLLRHWRLALVIILVAAAVLSPTSDVLNMMLFA
                     APMLVLYVISVFIAWLFARPRTAEA"
     gene            complement(2900..3331)
                     /gene="tatA"
                     /locus_tag="AC159P_08445"
     CDS             complement(2900..3331)
                     /gene="tatA"
                     /locus_tag="AC159P_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014261156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043953 - protein transport by the Tat
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HIR3695520.1"
                     /translation="MPLLFLEFIGTTELIVIAAVALLVFGPRKLPEIGRTIGKSLAEF
                     KRASEDFKRTWEYEVDMERSESETRVEPAAAARATLPDGEEAAAAVNPGEGFDNGPAP
                     AATEEPTVARTPKDYSTGPAAEAEEPSVEESSASGGKKDWL"
     gene            complement(3419..3784)
                     /locus_tag="AC159P_08450"
     CDS             complement(3419..3784)
                     /locus_tag="AC159P_08450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR3695521.1"
                     /translation="MQAQGGWRVEGVVDHFAVESEDLRRDVAEYERLGFSVETLYEDW
                     AMLRDARGFGLALLPPGSKHPPHIGIRVETREALEEAARRENRPVKEHRDRSLSFYTK
                     GVGGRAVEVIYYPPEYKQP"
     gene            complement(3899..4585)
                     /locus_tag="AC159P_08455"
     CDS             complement(3899..4585)
                     /locus_tag="AC159P_08455"
                     /inference="COORDINATES: protein motif:HMM:NF013077.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HIR3695522.1"
                     /translation="MQDKPTSFPTTRPDGSPLKPITEMLLERRATNSFTDEEVPEEYL
                     NAILELGAQAPSGYNLQPWRFVVVRDAENRQRLQKVAYNQPKVGQAPVVVIAVGMKEE
                     WKERAAEVFQEGAERGMGKPENSEKAAEGAIQFVSTLDPALWVNRHTMIAFTTMMLVA
                     ESYGFDTAPMEGFDAAAVKREFGIPEEGEVVALLAIGRAAQPDKPYTGRFELERIAYA
                     ERYGEAWKEK"
BASE COUNT          804 a         1495 c         1644 g          856 t
ORIGIN      
        1 ttgtagatct gcatgaagat gcccacgtcc tgcccgcgct tgaagacgcc cgcgacgttc
       61 gggatcacct tgtgctggcc gatggtgaac atccccggca gctgatcctt gagcgtctgc
      121 aacttgacgg cgagcaccat cgtcgaggcc gagagcttgt cggcctcgta cttcggcacg
      181 gtgaagccga cgggcttgat ctgctgcgag ccggagttga tgtcgcgcac cacgagatcg
      241 accttgtagg tgcccggcgg gagcggcacg acgcgctggt agacggactt gcgctcgcgc
      301 gtgtcggtca gctcttcgac cgtggcggtg gtgacgaccg ggtcttcgaa gctcgtcacg
      361 cggcggcccg cgaccgaggt cacgcgcccg tagatgttga ggcgcgcggt ctgcacgccg
      421 ccgatgtcgt cgaacttgag gtcgcggttg tcggcctgca cggtgatggc cgtaatcacg
      481 ctgttgtcgg actggcggaa gaagtccacg cgggcgtcaa cgtccaaaga gttcgggtct
      541 tcgacgggcg agttgacgag ggccagcagg ttcttctcca tcgcctcgtt gatctgcggc
      601 gggcgctgga gatcgacgat gcgctggagc cactcgaagg ggctgtcctg cacgcgcgcg
      661 cccggccccg agtagccgaa gccggtggcg cccgcgacgc ggtcggcctt gtcgctcatg
      721 ccgagctgct cgccgagcgt caggcccgcg ccggggatgt gcatcagcgc gtccttctcg
      781 ttgggcgagc gcgcgatgcg gtactcgccg gtgccggtcg gatcgacgaa ctcgatctcg
      841 acgccggagc cgacgcccgg caggtggcgg tagaaccaga tttcgaaggg gtaggtcgtc
      901 gtcgagccgc cgccgtggta cgagggccgg tcgtactggc cgcccatcgg gtgcgactcc
      961 ttgtcgttcg gcgggccgta catgatgtag atgcgcccgc ggtcggtctt ccagccggga
     1021 atgccggagg cgaagtgctg gttggcgtag gcgatgcgct cgaagtgctc ttcgaggtac
     1081 tcgttgacct cggtgtcggg gttcgggtcg cggcggcgcc agaaggcttc gatgtactgc
     1141 tcgcgctcct cgtcggtggc gagcttcttg aaggcccgct tctcgtcgga cgtgatgatg
     1201 tagaccacgt cctgctccat ccacttcttg aagatgtcgg tgcgctcctt cttgaccgtg
     1261 cgctcgcggt cgggcacgtc ctgcgcgtcc tgattcttct tcttgttctt gtcgttctgc
     1321 gcccacgcgg ttggggcggc ttgaaggatg aagagcagac tgagggctaa ggccagagag
     1381 cgcgtccagt gttgacgggc catcgctgta aactcctgtt tctggtgcac caagatttcc
     1441 gtaatggtcg cccgccgaac gtgtgcgggc atggtttgaa gtgtcctgcc gaaactcccg
     1501 cgaccggggc cacgaaaatg gcgacgcaag gatggccgct cggaagcgcg tcttacacgc
     1561 aaggctgccg aaaccctgtg catcgcccga ctttcgggca ggattttaga gcctttcgag
     1621 agacacagtc aagatgcccg aaatggcctt ccggttgccc ggaaggccgg ggcttacaaa
     1681 gggaaaaaag ggaaaaaggg gaaggggcga aaagggatag agcggaacgc tgcgggcgcg
     1741 gtctttcctt ttcgcctttt aaccttttcg ccttttaacc ccggattttt atgcctccgc
     1801 cgtgcggggc cgcgcgaaga gccaggcgat gaagacggag atgacgtaga ggacgagcat
     1861 cggggcggcg aagagcatca tgttgagcac gtccgaggtg ggcgagagca cggcggcggc
     1921 gacgaggatg atgacgagcg cgaggcgcca gtggcgcagc aggaagcccg cgttgacgat
     1981 gccgatgcgc gaaagcacgt aggtgacggc gggcatctgg aagacgacgc ccatcgccag
     2041 catgatgagg atgatgaagt cgaagtagtc cgtggcgttg aggaagaggc ggaagctgtc
     2101 ggccccgagg cccagcaggt acgtgaccgc cggggggaag agcacgtagt aggcgaaggc
     2161 cgcgccgatg aggaaggaga ccgtcgagag gaagatgaag ggcagcgcgt agccgcgctc
     2221 gtgcgggtag aggccgggcg cgatgaaggc ccagacctgc cagagcagga agggcaccga
     2281 gaggcagacc gccgcgtaga gagagacttg caggtagagc gagaaaggct cgatggcggt
     2341 gcggacgacg agccgctcgt cgcctgcgcc gccgccctcg cccgcgcgcg ccttgaagtc
     2401 cgcgggcagg cgcaccccct tcgggatgac ggtggccccc gcaaagacgg gttcgtccgt
     2461 gaagacgccc agctcgcccg cgcggtcgcg cgccacgcgc gccgccaccg aggcgccgac
     2521 ggggacgacg cccgtgccga gcttggtcgt ctcggcgaag acgtagcggc ccgtgtcgcc
     2581 ctcctgcaag cggtcgagcg tgagaatctc ctcgccgccc gtcaagccgt gcagcgggac
     2641 ggggcggcgg gcggcgtcgg ccagcgcgcg ccgcacgggc accgaaagaa agttgtagat
     2701 gtggtcggag acgaaccagc acgcgacgag cgcgacgaag acgaaggcga ccgagcgcat
     2761 cagccggcgg cgcagctcgt cgaggtggtc gaggaaagac atctgcgcgc cgagcctcga
     2821 ctcgtcgcgc tcttcgtcct gttcggtgtc ggctaaggat gatgccatcg ggaagatttc
     2881 ggcgggcggg ggccgaagcc tagagccagt ccttcttgcc gccggacgcg gagctttcct
     2941 ccacggaggg ttcttcggct tcagccgcgg ggccggtcga gtaatccttc ggggtccgcg
     3001 ccaccgtcgg ctcttccgtc gccgcggggg cggggccgtt gtcgaagcct tcgccggggt
     3061 tgacggcggc ggcggcttct tcgccgtcgg gcagtgtggc gcgcgccgcc gccgccggct
     3121 cgacgcgcgt ctcgctctcg gagcgctcca tatcgacctc gtactcccac gtccgcttga
     3181 aatcctcgga cgcgcgcttg aactcggcga ggctcttgcc gatggtgcgc ccgatctcgg
     3241 ggagcttgcg cggcccgaag accagcagcg ccaccgccgc gatgacgatc agctcggtcg
     3301 tcccgatgaa ctcaagaaag agaagaggca taagacacat caagagccgt gaaccgtgaa
     3361 ccgtaaaccg tgacaagtga aaaggttttt tatcctttca gggtttacgg ttcacggttc
     3421 acggctgttt gtattccggc gggtagtaga tgacttcgac ggcgcgaccg ccgacgccct
     3481 tcgtgtagaa ggagagcgag cggtcgcggt gctccttgac ggggcggttc tcgcgccgcg
     3541 ccgcctcttc cagcgcctcg cgcgtctcga cgcggatgcc tatgtgcggc gggtgcttcg
     3601 agccgggcgg caagagcgcg aggccgaagc cgcgcgcgtc gcgcagcatc gcccagtctt
     3661 cgtacagcgt ctcgacgctg aagccgagcc gctcgtattc cgccacgtcg cgccgcaagt
     3721 cctccgactc gaccgcgaag tgatccacca cgccctcgac gcgccacccg ccctgtgcct
     3781 gcatcacgcg acccctttca agaaacgatg aatgcggaat gatgaatgat gaatcaaagg
     3841 cagcttgttc gatgacgggc accgtgcttc tcattcatca ttccgcgttc atcgttcatc
     3901 atttctcttt ccacgcttcg ccgtagcgct cggcgtaggc gatgcgctcc agctcgaagc
     3961 ggcccgtgta gggcttgtcg ggctgcgcgg cgcgaccgat ggccaagagc gcgaccacct
     4021 cgccctcttc ggggatgccg aactcgcgct tgaccgccgc cgcgtcgaag ccctccatcg
     4081 gcgcggtgtc gaagccgtac gactcggcca cgagcatcat cgtcgtgaag gcgatcatcg
     4141 tgtggcggtt gacccacagc gccgggtcga gcgtcgagac gaactggatc gcgccctcgg
     4201 cggccttctc ggagttttcg ggcttgccca tgccgcgctc ggcgccctct tggaagacct
     4261 ccgcggcccg ctccttccac tcctccttca tgccgacggc gatgacgacg accggcgcct
     4321 gccccacctt cggctggttg taggcgacct tttggaggcg ctggcggttc tccgcgtcgc
     4381 gcaccacgac gaagcgccac ggctgtagat tataaccgga tggggcctgc gccccgagtt
     4441 cgaggatggc gttcaggtac tcttccggca cctcctcgtc ggtgaagctg ttcgtcgcgc
     4501 ggcgttcgag cagcatctct gtgatcggct tcaacggcga cccgtcgggc cgcgtcgtcg
     4561 gaaaactcgt cggcttgtcc tgcatctcgc atccccctgc acttgaatgg tgccgcgatt
     4621 gtagcgcaaa agacaagcgg gcagtaggca gcaggcagca ggcagcaaat acaattcatg
     4681 attcgacttg agatcgaaga ttacgaattt caactccgag agttctttac tgcccgctgc
     4741 ccgctgcccg ctgcccgctg aactgtcttg acacgggcgc cgccgatgcg cgaacctcg
//
LOCUS       DBNGOH010000429         5308 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3365346, whole genome shotgun sequence.
ACCESSION   DBNGOH010000429 DBNGOH010000000
VERSION     DBNGOH010000429.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5308)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5308)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5308
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3365346"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            219..1853
                     /locus_tag="AC159P_08460"
     CDS             219..1853
                     /locus_tag="AC159P_08460"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR3695523.1"
                     /translation="MSVPLFPVSFMSPASDELFRKYFVEAIAKTSETVGPLYRLDAID
                     WTVLAIYFTILTVLALYGMYRIKQVFDFWRYRRLQPEPQRLFAEDELPVVTVQLPLFN
                     EMYVVERLLAAVAAVDYPRDRFEIQVLDDSTDETREVASRAVERYRREGFDISYIHRD
                     DRTGFKAGALENGMKTAKGELVAIFDADFVPKPDCLRKLVHFFTDPLTACAQMRWSHI
                     NGSYNLLTRLQTILLDGHFVVEQTVRNRTGGFFNFNGTAGVWRRRAIELSGGWQHDTL
                     TEDTDLSFRAQLMGWRFVYLLDEDAPSEVPVEINAFKAQQRRWAKGVTEVGLKLYPRI
                     WRSALPFRVKLEMFFRLTGNISYPLMIVTSFLQFPLLLVRYNQGLYHLMLLDVPLLFF
                     STLSVVLFYGSAIWYLDRPHARRRLLHLPLVMGLGIGLAFSNARAVLEALFRVKTEFV
                     RTPKYKVEGASDETWKRKKYKRRSGLLPLFELASACYFLLAIAYAARMEMWGTIPFLS
                     LFCFGYGYMGVMSLLQTTSARRLRDLFRPAAPALKQ"
     gene            2121..5195
                     /locus_tag="AC159P_08465"
     CDS             2121..5195
                     /locus_tag="AC159P_08465"
                     /inference="COORDINATES: protein motif:HMM:NF051698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR3695524.1"
                     /translation="MRKLPPVISRALSSCACVVLCAAAALAQSQATTGNIEGRVLDPN
                     TAVVPGASVTATNQETGFERAATADDDGNYRLILLPPGRYTVKAAAANFAAAQLGDVI
                     VTVGGATALDLTLGVGGTTTDITVTSEAPVVELTRTSVSTTVNERAIENLPVNGRNYL
                     DFATLTPSVVRDQNREGDLSVGGLKGTLNSLQVDGSDNNNTFFGQSFGRTGVRPPYQF
                     SEESVQEFQVNQNGFSAEFGRAGGAVINVVTKSGTNEFHGGLFEYFRDESLNANTPQI
                     KAQQFQRGLPNRRPKLQINQFGGRIGGPIVKNRAFFFFTYDGQRQDLPNIVDPPGLFT
                     QPAAVQTLLVPKVGAYQVNRNQDVYMAKGDIALSRGNQLSLRFNQQNFTGSNNEFTGS
                     LGTEEHSGDSIAKTTTFSGSLTSTLTSSVLNELRFQFARDREPGTANSDAPEAQINTG
                     GGNLLIGRNNFSPRETTIKRAQLIDNISFVRGAHNFKTGFDFNFDRIFNFFPGFFSGQ
                     YTFPSYAAFAANQPSAFTQRFALPNTSGAETEPDSTDYAVFFQDDWRVTPRFTLNLGV
                     RYDYQKLARPPLTNPDPVLLAAGLDTGRLSNDSNNLAPRAGFSYSPDEKTVVRGGYGI
                     FYGRTTAIMLGTAHSGNGIQTTGLTLSSNAAIVAAGLTYPNVFTAIPSGLVPPRPDLF
                     LFADDYAQPYVQQGRLGVEREVFENVSLSVSYLYFRGVHLSRTRDINLLAPVATTAVD
                     ASGNSYTVQRFPGTGANAAAQFTSTTPLRPFANYNRIQLFESTANSRYNALAVQLNRR
                     FSNNLQFLVSYTFSKAEDDKPDQTAVVPGGGDDFKLAQNSFDLRDEWGRADSDVPHRF
                     VFSPVYEIGTIKWSENPIARALFSNYTLSGIAQLQSGFVYSAQIGTDLNRDGNSRNDR
                     VPGTRRNGFRTPNVYQFDARIARNIPFGENRRLRLILEGFNIFNRANVGLAPGGGYFS
                     FVNINRFRGFTATSVGGTNTLTLLDPLPATPFGTDRAIITPRQLQLAVKFDF"
BASE COUNT          894 a         1978 c         1532 g          904 t
ORIGIN      
        1 gtggacgggg gcggcgcccc cgcgtcccgt tgtgcgacgg gcacactttc ggctacaatt
       61 cgtgcatcta agcgggcagc gtcggcaaca gaacccggcg ctgctctttg cttatgtgat
      121 ggcggcggcg gggcagcgtc ccggcaccgt ccgtcgcgcg ccgcgccgcc atgagacttc
      181 gcgccggcgc gtttcccgaa gcccgtgaga gatcgaagat gtctgtgcct ttattccccg
      241 tttcgttcat gtcgcccgct tccgacgagc tgttccgcaa atacttcgtg gaggccatcg
      301 ccaagacttc ggagacggtc ggcccgctct accgcctcga cgccatcgac tggacggtgc
      361 tcgccatcta cttcaccatc ctgacggtgc tcgcgctcta cggcatgtac cgcatcaagc
      421 aggtcttcga cttctggcgc taccgccgcc tgcaacccga gccgcagcgc ctcttcgccg
      481 aggacgagct gcccgtcgtc accgtgcagc tcccgctctt caacgagatg tacgtcgtcg
      541 agcggctgct cgcggcggtg gcggcggtcg attacccgcg cgaccgtttc gagattcagg
      601 tcctggacga ctcgaccgac gagacgcgag aggtggcgag ccgcgccgtc gagcgctacc
      661 gccgggaagg cttcgacatc tcctacatcc accgcgacga ccgcacgggc ttcaaggcgg
      721 gcgcgctgga gaacggcatg aagacggcga agggcgagct ggtcgccatc ttcgacgccg
      781 acttcgtgcc caagcccgac tgcctgcgca agctcgtcca cttcttcaca gaccccttga
      841 cggcctgcgc gcagatgcgc tggtcgcaca tcaacggctc ttacaatttg ctgacgcggc
      901 tgcagacgat tcttctggac ggccacttcg tcgtcgagca gacggtcagg aaccgcacgg
      961 gcggcttctt caacttcaac gggacggcgg gcgtctggcg gcggcgcgcc atcgagctgt
     1021 cgggcggctg gcagcacgac acgctgaccg aagacacgga cctctctttc cgcgcgcagc
     1081 tcatgggctg gcgcttcgtc tacctcctgg acgaggacgc gccttcggag gtgccggtcg
     1141 agatcaacgc cttcaaggcg cagcagcgcc gctgggcgaa gggcgtcacc gaggtcggcc
     1201 tcaagctcta cccgcgcatc tggcgctcgg cgctcccctt tcgcgtcaag ctcgaaatgt
     1261 tcttccgcct gacgggcaac atcagctacc cgctgatgat cgtcacgagc ttccttcagt
     1321 tcccgctcct gctcgtccgc tacaaccagg gcctctatca cctgatgctg ctggacgtgc
     1381 cgctcctgtt cttctcgacg ctctcggtcg tgctcttcta cggctcggcc atctggtatc
     1441 tcgaccgccc gcacgcgcgc cgccgcctgc tgcacctgcc gctggtgatg ggcctcggca
     1501 tcggcctcgc gttctcgaac gcgcgcgccg tcttggaagc gctcttccgc gtcaagaccg
     1561 agttcgtccg cacgcccaag tacaaggtcg agggcgcgag cgacgagacg tggaagcgga
     1621 agaagtacaa acgccggagc gggttgctcc cgctcttcga gttggcctcg gcctgctatt
     1681 tcctgctcgc catcgcctac gccgcccgca tggagatgtg gggcacgatc cctttcctct
     1741 cgctcttctg cttcggctac ggctacatgg gcgtgatgag cctcttgcag acgaccagcg
     1801 cccgccgcct gcgcgacctc ttccgtcccg ccgcgcctgc gctcaaacag tgagtgtcgc
     1861 gcggctttct tccaacacgg agggcacgga ggagacacgg agggcacgga gaagaccaag
     1921 ctgctttctc cgtgccctcc gtgttgaacc cggcctcacg ccagactcga ccgcgatggt
     1981 tctcggcggc gcggaacatc ctccttgacc gcgcccgcgc gccgtcatag tatctcggct
     2041 ctgacgcgtc acttcgtgga cgattgttca gtcgccaccc cgtcttgctg tccgctccga
     2101 acgacagaga ggaatccatg ttgagaaaac ttccccccgt catatcgcgc gcactctctt
     2161 cgtgcgcgtg cgttgttctt tgtgccgccg cggcgctcgc gcagtcgcag gcgacgaccg
     2221 gcaacatcga gggccgcgtg ctcgacccga acactgccgt cgtccccggc gcgagcgtga
     2281 ccgccacgaa tcaggagacc ggcttcgagc gcgccgccac ggcggacgat gacggcaact
     2341 accgcctgat cctgctgccg ccgggccgct acaccgtcaa ggctgctgcc gcgaacttcg
     2401 ccgccgcgca gctcggagac gtaatcgtca cggtcggcgg cgcgacggcg ctcgacctga
     2461 cgctcggcgt cggcggcacg accaccgaca tcacggtcac gagcgaagcc cccgtggtcg
     2521 agctgacgcg cacttccgtc tcgacgacgg tcaacgagcg cgccatcgag aacctgccgg
     2581 tcaacgggcg caactacctc gacttcgcca cgctcacgcc ctcggtcgtc cgcgaccaga
     2641 accgcgaagg cgacctttcg gtcggcggcc tgaagggcac cctgaacagc ctccaggtgg
     2701 acggctcgga caacaacaac accttcttcg gccagtcgtt cgggcgcacc ggcgtccgcc
     2761 cgccctacca gttctccgaa gagtccgtgc aggagtttca ggtcaaccag aacggcttct
     2821 cggcggagtt cgggcgcgcg ggcggcgccg tcatcaacgt cgtcaccaag tcgggcacga
     2881 acgagttcca cggcgggctg ttcgagtact tccgcgacga atccctgaac gccaacacgc
     2941 cgcagatcaa ggcgcagcag ttccagcgcg gcctgccgaa ccgccgaccg aagttgcaga
     3001 tcaaccagtt cggcgggcgc atcggcggcc ccatcgtcaa aaaccgggcc ttcttcttct
     3061 tcacctacga cggccagcgg caagacctcc cgaacatcgt cgatccgccc ggcctcttca
     3121 cgcagccggc ggcagtccag acgctgctgg tgccgaaggt cggcgcctat caggtcaacc
     3181 gcaatcagga cgtttacatg gcgaagggcg acatcgcgct gagccgcggc aaccagttgt
     3241 ctttgcgctt caaccagcag aacttcaccg gctcgaacaa cgagttcacc ggctcgctcg
     3301 gcaccgaaga gcactccggc gacagtatcg ccaagacgac gaccttctcc ggctcgctca
     3361 cctcgaccct gacctcgtcc gtgctcaacg agctgcgctt ccagttcgcg cgcgaccgcg
     3421 agccgggcac ggccaacagc gacgcccccg aggcgcagat caacacgggg ggcggcaacc
     3481 tcctcatcgg tcgcaacaac ttcagcccgc gcgagacgac catcaagcgc gcgcagctca
     3541 tcgacaacat ctccttcgtc cgcggcgcgc acaatttcaa gaccggcttc gacttcaact
     3601 tcgaccgcat cttcaacttc ttccccggct tcttctcggg gcagtacacc ttcccctctt
     3661 acgcggcctt cgccgccaac cagccctcgg ccttcacgca gcggttcgcg ctgccgaata
     3721 cctccggggc cgagaccgaa cccgacagca cggactacgc agtcttcttc caggacgact
     3781 ggcgcgtgac gcccagattc accctcaacc tcggcgtgcg ctacgactac cagaagctcg
     3841 cgcgcccgcc gctcacgaac cccgacccgg tcctcctcgc cgcgggcctc gacacgggcc
     3901 ggctctcgaa cgactcgaac aacctcgcac cgcgcgcggg cttcagctac agccccgacg
     3961 agaagacggt cgtgcgcggc ggctacggca tcttctacgg gcggacgacc gccatcatgc
     4021 tcggcacggc gcactccggg aacggcatcc agacgaccgg cctcaccctt tcgagcaacg
     4081 cggccatcgt cgccgcgggc ctcacctacc cgaacgtctt caccgcgatc ccttcgggcc
     4141 tcgtgccgcc gcgccccgac ctcttcctct tcgcggacga ctacgcgcag ccctacgtgc
     4201 agcagggccg tctcggcgtc gagcgcgagg tcttcgagaa cgtcagcctc tcggtctcgt
     4261 acctctactt ccgcggcgtg cacctctcgc gcacgcgcga catcaacctc ctcgcgcccg
     4321 tcgcgacgac cgccgtggac gcctcgggca acagctacac cgtccagcgc ttccccggca
     4381 cgggcgccaa cgcggcggcg cagttcacct cgaccacgcc gctgcggccc ttcgccaact
     4441 acaaccgcat ccagctcttc gagagcaccg ccaactcgcg ctacaacgcg ctggccgtgc
     4501 agctcaaccg ccgcttctcg aacaacctcc agttcctcgt ctcctacacc ttctcgaagg
     4561 ccgaggacga caagcccgac cagacggcgg tcgtgcccgg cggcggcgac gacttcaagt
     4621 tggcgcagaa ctccttcgat ctcagggacg agtgggggcg ggccgactcg gacgtgccgc
     4681 accgcttcgt cttcagcccg gtctacgaga tcggcaccat caagtggagc gagaacccaa
     4741 tcgcgcgcgc cctcttcagc aactacacgc tctccggcat cgcgcagctc cagtccggct
     4801 tcgtctactc ggcgcagatc ggcaccgatc tgaaccgcga cggcaactcg cgcaacgacc
     4861 gcgtgcccgg cacccgccgc aacgggttcc gcacgccgaa cgtctaccag ttcgacgcgc
     4921 gcatcgcccg caacatcccc ttcggcgaga atcggcggct gcggctcatc ctcgaaggct
     4981 tcaacatctt caaccgggcc aacgtcggcc tcgcgccggg cggcggctac ttctcgttcg
     5041 tcaacatcaa ccgcttccgc ggcttcacgg cgacctccgt cggcggcacg aacacgctga
     5101 cgctgctcga cccgctcccc gcgacgccct tcggcaccga ccgcgccatc atcaccccgc
     5161 ggcagttgca gctcgccgtc aagttcgact tctaaagcgg taggcagtag gcggtaggca
     5221 gtagccagca aaggcaagag gcttgagatg atgaatctca agcctctttt actgcttatg
     5281 acttcttctg tccttactgc ctactgcc
//
LOCUS       DBNGOH010000430         1925 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2998319, whole genome shotgun sequence.
ACCESSION   DBNGOH010000430 DBNGOH010000000
VERSION     DBNGOH010000430.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1925)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1925)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1925
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2998319"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..269
                     /locus_tag="AC159P_08470"
     CDS             <1..269
                     /locus_tag="AC159P_08470"
                     /inference="COORDINATES: protein motif:HMM:NF015436.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HIR3695525.1"
                     /translation="GFLLAEAGRLLKPGDRVEHEGALFRVERVDRRRIRWVRFTPAPP
                     PAAETEGDNSSAATLHAGALMFGTSMTHAGVELSAQAEFLLAVI"
     gene            complement(358..687)
                     /locus_tag="AC159P_08475"
     CDS             complement(358..687)
                     /locus_tag="AC159P_08475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695526.1"
                     /translation="MAIRRRRHKHGREGRKKHTDELSAMLAPLEELMPEAAPPPGQST
                     NGDYHDATIRVDSSSVKTPEQLLDEDEPREGLRIFRLEPLVVVILCVMLAFIAFVAWQ
                     ITLMPAK"
     gene            complement(678..1649)
                     /gene="corA"
                     /locus_tag="AC159P_08480"
     CDS             complement(678..1649)
                     /gene="corA"
                     /locus_tag="AC159P_08480"
                     /inference="COORDINATES: protein motif:HMM:NF013694.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HIR3695527.1"
                     /translation="MITTTCLDSKTRKFINDFPAADISDYCLSEQNVVWADVSDPTGK
                     DFEELAEEFNFHPLSIEDCRNAHQRPKVEEYPGYYFIVLYESELVGADDDLELRELNI
                     FLGPNYLVTVHSRPIRAIETAKRLWGEWTDRSGHGAGLLAYLLIDAIVDDYLPLLDVL
                     SDHMDDLEDNIFGDFKPESIQDIFRIKKQLLYLRRSITPLRDVFNTMLRREQPIFPRE
                     TLVYFQDVFDHIIRVADTIDSLRDMLGSTMDAYLSVSGNRMNQVMKRLTSISTILMSV
                     TLVAGVYGMNFDFMPELKWRFGYIFALLSMVGIGLVIYFYLRKIKWL"
     gene            1765..>1925
                     /locus_tag="AC159P_08485"
     CDS             1765..>1925
                     /locus_tag="AC159P_08485"
                     /inference="COORDINATES: protein motif:HMM:NF021161.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2391 family protein"
                     /protein_id="HIR3695528.1"
                     /translation="MRTVSQSLREYARGVAGGLLFSLPLLYTMEVWWAGFVAHPWRLA
                     LYVAGTFLLL"
BASE COUNT          322 a          572 c          676 g          355 t
ORIGIN      
        1 cggggttcct gctggccgag gccgggcggc tgctgaagcc gggcgaccgc gtcgagcacg
       61 agggcgcgct cttccgcgtc gagcgcgtag accgccgccg catccgctgg gtgcgcttca
      121 cgcccgcccc gccgcccgcc gccgagacgg aaggcgacaa cagcagcgcc gccacgctcc
      181 atgccggcgc gctgatgttc ggcacgtcga tgacgcacgc gggcgtggag ctgagcgcgc
      241 aggcggagtt cctgctcgcc gtcatatagg cgaaggggaa aaaggttaaa aggggaaaag
      301 gcgaaaaggg gaagacaggc ttcttccttt tcgcctttta acctttcccc ctcctgctta
      361 cttcgcgggc atgagggtga tctgccaggc gacgaaggcg atgaaggcga gcatgacgca
      421 gaggatgacg acgacgaggg gttcgaggcg gaagatgcgc aggccctcgc gcggctcgtc
      481 ctcgtcgagg agctgctcgg gcgtcttgac gctgctgcta tcgacgcgga tggtggcgtc
      541 gtggtagtcg ccgttggtgg actggcccgg cggcggcgcg gcctccggca tcagctcttc
      601 gagcggggcg agcatcgccg acagctcgtc cgtgtgtttt ttgcgccctt cgcgcccgtg
      661 cttgtggcgc cgtctcctta tagccatttg atcttcctca ggtagaagta gatgacgaga
      721 ccgatgccga ccatcgagag cagcgcgaag atgtagccga agcgccactt cagctcgggc
      781 atgaagtcga agttcatccc gtagacgccc gccaccagcg tcaccgacat gaggatggtc
      841 gagatcgagg tgagccgctt catcacctga ttcatgcggt tgccggagac agagaggtag
      901 gcgtccatcg tcgagccgag catgtcgcgc aaagagtcga tggtgtcggc gacgcggatg
      961 atgtggtcga acacgtcctg gaagtagacg agcgtttcgc gcgggaagat gggctgctcg
     1021 cgccggagca tggtgttgaa aacgtcgcgc aagggggtga tcgagcggcg caggtagagg
     1081 agctgcttct tgatgcggaa gatgtcctgg atggactcgg gcttgaagtc gccgaagatg
     1141 ttgtcttcga ggtcgtccat gtgatccgag agcacgtcca ggagcggcag gtagtcgtcc
     1201 acgatggcgt cgatcaggag gtacgcgagc agcccggcgc catgccccga gcggtcggtc
     1261 cactcgcccc acaggcgctt cgccgtctcg atggcgcgga tggggcggct gtggacggtg
     1321 acgaggtagt tggggccgag gaagatgttc agctcgcgca gttcgaggtc gtcgtcggcg
     1381 ccgaccagct ccgactcgta gagcacgatg aagtagtagc cggggtactc ctcgaccttc
     1441 gggcgctggt gcgcgttgcg gcagtcttcg atggagagcg ggtggaagtt gaactcctcg
     1501 gccagctcct cgaagtcttt gccggtcggg tcggacacgt cggcccagac gacgttttgc
     1561 tcgctcaggc aataatcgct aatatcagcc gccgggaagt cgttgatgaa cttccgggtc
     1621 ttgctgtcga ggcaggtggt cgtaatcatc cgctcggctc cgcagtctcc gcgattctcg
     1681 ggtgcgccgc cgagtatacc aattcaagtc cgctcccgct caaattcgtg cggcggccaa
     1741 gcgcggcgcc gcaccccgat ccggatgcgc acggtcagcc aatcactgcg cgagtacgcg
     1801 cgcggcgtcg caggcgggct gctcttcagc ctgccgctgc tctacacgat ggaggtgtgg
     1861 tgggcgggct tcgtcgcgca cccgtggcgg ctggcgctct acgtcgcggg caccttcctg
     1921 ctcct
//
LOCUS       DBNGOH010000431         4076 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2264908, whole genome shotgun sequence.
ACCESSION   DBNGOH010000431 DBNGOH010000000
VERSION     DBNGOH010000431.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4076)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4076)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4076
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2264908"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..934)
                     /locus_tag="AC159P_08490"
     CDS             complement(<1..934)
                     /locus_tag="AC159P_08490"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HIR3695529.1"
                     /translation="MSTHSGNANANGSGQTGRVVQIIGPVLDVEFGDGYLPPIYQALR
                     ITSEGFDVPTPIDIIVEVEQHLGEGRVRTVSMQPTEGLVRGMVATDTGGPITMPVGET
                     TLGRVLNVIGQPVDNLGPVNSEQRYPIHRHAPLLEEQSTELEMFETGIKVVDLIQPFL
                     RGGKIGLFGGAGVGKTVLIMELINNVAKAHSGFSVFAGVGERTREGNDLLREMVESKV
                     INFGEGFYEHYESSGDFDMTKVDMQAIGESKVALVYGQMTEPPGARLRVALSALTVAE
                     YFRDQGLDVLLFIDNIFRFTQAGSEVSALLGRMPS"
     gene            complement(1176..1442)
                     /locus_tag="AC159P_08495"
     CDS             complement(1176..1442)
                     /locus_tag="AC159P_08495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695530.1"
                     /translation="MEINDDAKEIVKELGAAINAAVERSPDVAEAIERLREAGYDMEL
                     TLRLEIGLRERDGGDEDDPARLELTDEDRRTLRNMRIRFDDAED"
     gene            complement(1645..2796)
                     /locus_tag="AC159P_08500"
     CDS             complement(1645..2796)
                     /locus_tag="AC159P_08500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695531.1"
                     /translation="MRKTLFSVLPLVVLLAPSFAPVNLFSLALFRRPAKTAAVREAAP
                     EEPAVAALRGRVAEAKLLLRSRPAPASTEEVTLAVEETAGGQIHLLTLPKEDYLREGA
                     EVALLSSEGARLALRVVRPNYVNTVVRVRDAAGRELRPLVVQYPIEKGGKLTEVAYYT
                     SAHPATASAPLVRGGSLYVRGKLDAAARLLAAKGKKISPEVVDVAERLCVVEHTDHKR
                     FKKEQHAQLFEEIYALYALNSGDTYRYSVSSAGAGGMVQMIPPTYKAMRERHSDVSMA
                     EDFVTAMRDHDNAAAVMLLYLQDTWNGLLKEEEVRHALDTNLATQAELLAAGYNSNPM
                     RLAGYLKRGGDSWRTLIPAETQMYLRIYASVEENVMRPRAAQTFAGPGA"
     gene            3065..>4076
                     /locus_tag="AC159P_08505"
     CDS             3065..>4076
                     /locus_tag="AC159P_08505"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011984605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0006637 - acyl-CoA metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA mutase large subunit family protein"
                     /protein_id="HIR3695532.1"
                     /translation="MATKTDAGRERRDHFETSSGIPLPNDFNPSNTEPPDYERDLGAP
                     GEFPYTRGVRRSMYRGRFWTMRQYAGYATAEESNARYKYLLAQGTTGLSVAFDLPTQI
                     GLDSDDPLAAGEVGKVGVAIDSIEDMERLFAGIPLGEVSTSMTINATAAILLCLYVAV
                     GKRQSVAPDKLQGTIQNDILKEYIARGTYIYPPGPSLRLVTDTFAYCAREVPNWNTIS
                     ISGYHIREAGSTAVQEVAFTLADAVAYVEAAVASGLRIDEFAPRLSFFFNSHNNLLEE
                     VAKFRAARRLWARLLRDRFHARDPRSLMLRFHTQTAGSTLTAQQPEVNVVRTAIQALA
                     AVL"
BASE COUNT          677 a         1336 c         1333 g          730 t
ORIGIN      
        1 tcgaaggcat ccgcccgagg agcgcggaca cttcggagcc ggcctgcgtg aagcggaaga
       61 tgttgtcgat gaagaggagc acgtccaggc cctggtcgcg gaagtactcg gcgaccgtca
      121 gcgccgagag cgcgacgcgc aggcgcgcgc ccggcggctc ggtcatctgc ccgtagacga
      181 gcgccacctt cgactcgccg atggcctgca tgtccacctt cgtcatgtcg aagtcgcccg
      241 acgactcgta gtgctcgtag aagccctcgc cgaagttgat gaccttcgac tcgaccatct
      301 cgcgcaagag gtcgttgccc tcgcgcgtgc gctcgccgac cccggcgaag accgagaagc
      361 cggagtgcgc cttggcgacg ttgttgatca gctccatgat gagcacggtc ttgccgacgc
      421 ccgcgccgcc gaacagaccg atcttgccgc cgcgcaggaa gggctgaatc aggtcaacga
      481 ccttgatgcc cgtctcgaac atttcgagtt cggtggactg ctcttccaga agcggcgcgt
      541 ggcggtggat gggataacgc tgctcggagt tgaccgggcc gaggttgtcc acaggctgtc
      601 caatcacgtt cagcacgcgc cccaaggtcg tctcgccgac cggcatcgtg atcgggccgc
      661 cggtgtcggt cgccaccatg ccgcgcacga ggccctcggt cggctgcatc gagacggtgc
      721 ggacgcgacc ctcgccgagg tgctgctcga cctcgacgat gatgtcgatg ggcgtcggca
      781 cgtcgaagcc ttccgaggtg atgcgaagcg cctgatagat gggcgggaga tagccgtcgc
      841 cgaactcgac atcgaggacg gggccgatga tctgcacgac gcggcccgtc tggccgctgc
      901 cgttcgcgtt cgcgtttccg ctgtgagtgg acatactttt ccgtaaagct cctctgttgt
      961 gttaaaggcc gaaggcgaga aaatacactg tgatttaagg tacaaaggtt agcacacgcc
     1021 gcgagcggaa ggcaaagcgg agtaggcagt aggcagtagg cagtaggcgg taggcggtag
     1081 gcagtaacaa aggaagaacg cagaggcttg gggctttact tctcaagccc ctgacatctt
     1141 ctgctttgac tgcctaccgc ctactgccta ctgcttcagt cttcggcgtc gtcgaagcgg
     1201 atgcgcatgt tgcggagcgt gcggcggtct tcgtcggtga gttcgaggcg ggcggggtcg
     1261 tcttcgtcgc cgccgtcgcg ctcgcgcagg ccgatctcga ggcgcagcgt cagctccatg
     1321 tcgtagcccg cttcgcgcag gcgctcgatg gcttcggcca cgtcgggcga acgctcgacc
     1381 gccgcgttga tggccgcgcc cagttccttc acgatctcct tagcgtcgtc gttgatctcc
     1441 aaaaagtgac ctctcgcgtt cggtattcga ttttcggtga agggcgcgcc gtttgttcga
     1501 tgctacgcga gccgcgtcgg ggcggtcaac cgacggaagg ggaaaggaaa ggggaaaagg
     1561 ttaataaggg gaaaaggcga aagcaaacga cttgccgcct tccccttttc gccttttccc
     1621 cttttaacct tttccccctc tgccttacgc gccggggccg gcgaacgtct gtgcggcgcg
     1681 ggggcgcatg acgttctcct cgacggaggc gtagatgcgg aggtacatct gcgtctcggc
     1741 ggggatgagc gtgcgccacg agtcgccgcc gcgcttgagg tagccggcga gccgcatcgg
     1801 gttcgagttg tagccggcgg ccagcagctc ggcctgcgtg gcgaggttcg tgtcgagcgc
     1861 gtggcgcact tcctcttctt tgaggaggcc gttccaggtg tcctgcaagt agaggagcat
     1921 gacggcggcg gcgttgtcgt ggtcgcgcat cgcggtgacg aagtcttcgg ccatcgacac
     1981 gtccgagtgg cgctcgcgca tcgccttgta ggtcggcggg atcatctgca ccatgccgcc
     2041 cgcgcccgcg ctcgacaccg agtagcggta ggtgtcgccg gagttgagcg cgtagagcgc
     2101 gtaaatctct tcgaagagct gggcgtgctg ctctttcttg aagcgcttgt ggtcggtgtg
     2161 ctcgacgacg cagaggcgct cggccacgtc caccacctcg ggcgaaatct tcttgccctt
     2221 cgcggccagc aggcgggccg ccgcgtcgag cttgccgcgg acgtagaggc tcccgccgcg
     2281 caccagcggc gcggaggcgg tggccgggtg cgccgaagtg tagtaggcca cctcggtcag
     2341 cttgccgccc ttttcgatgg ggtactggac gacaagcggg cgcagctcgc ggcccgccgc
     2401 gtcccgcacg cggacgaccg tgttgacgta gttcgggcgg acgacgcgca gcgcgaggcg
     2461 cgcgccctcg gacgaaagga gcgcgacctc tgcgccctcg cgcaggtagt cttctttagg
     2521 cagcgtgagc aggtgaatct gcccgcccgc cgtctcctcg acggcgagcg tgacctcttc
     2581 ggtggacgcg ggcgcgggcc gcgagcggag cagcagcttc gcctcggcga cgcgaccgcg
     2641 cagcgccgcg accgccggct cttcgggcgc agcctcgcgc accgccgccg tcttcgcggg
     2701 gcgtctgaac agggcgagcg agaagaggtt gacgggcgcg aagctcgggg caagcaacac
     2761 cacgagcggg agaacagaaa acagcgtctt tctcatagtt atcgggtcgc ctgatttttt
     2821 acggaaaacc gaagtgttca ggggttcggg aagagactat tcagacgccc ccgaagccct
     2881 cgcagttgta ccctgcgcgc cgttcaattt gcccccgccc gtgcacagaa agcgtgcatc
     2941 tgtgctaggc tggagacaat agggggaaag ggttaagagg ggaaaaggcg aaaaggtttt
     3001 tgtcttctcc ctcttcgcct cttcaccttt tcccctcttc cccttgcttt tcggtgtgaa
     3061 gcttatggcg acgaaaacag acgcgggacg cgagcggcgc gaccacttcg agacctcttc
     3121 gggcatcccc ctgccgaacg acttcaaccc ttcgaacacg gagccgcccg actacgagcg
     3181 cgacctcggc gcgcccggcg agtttccgta cacgcgcggc gtgcgccgca gcatgtaccg
     3241 cgggcgcttc tggacgatgc gccagtacgc gggctacgcg accgccgaag agtcgaacgc
     3301 gcgctacaag tacctgctcg cgcagggcac gaccggcctc tcggtcgcct tcgatttgcc
     3361 gacgcagatc ggcttggatt ccgacgaccc gctcgccgcc ggggaggtcg gcaaggtcgg
     3421 cgtcgccatc gattccatcg aagacatgga gcgcctcttc gcaggcatcc cgctcgggga
     3481 agtctccacc tcgatgacca tcaacgcgac cgccgccatc ctgctctgcc tctacgtcgc
     3541 cgtcggcaaa cggcagtcgg tcgcgcccga caagcttcag ggcaccatcc agaacgacat
     3601 cctcaaggag tacatcgcgc gcggcaccta catctacccg cccggccctt cgctgcggct
     3661 cgtcacggac accttcgcct actgcgcccg cgaggtgccg aactggaaca cgatttcgat
     3721 cagcggctat cacatccgcg aggccggctc gaccgccgtg caggaggtcg ccttcacgct
     3781 cgccgacgcc gtcgcctacg tcgaggcggc ggtcgcctcc ggcctccgga tcgacgagtt
     3841 cgcgccgcgc ctctccttct tcttcaactc gcacaacaac cttctggaag aggtcgccaa
     3901 gttccgcgcc gcgcggagac tctgggcgcg acttttgcgc gaccgcttcc acgcgcgcga
     3961 cccgcgctcg ttgatgctcc gcttccacac acagaccgcg ggctcgaccc tcaccgcgca
     4021 gcagcccgag gtcaacgtcg tccggacggc catccaggcg ctcgccgccg tgctcg
//
LOCUS       DBNGOH010000432         8309 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_798406, whole genome shotgun sequence.
ACCESSION   DBNGOH010000432 DBNGOH010000000
VERSION     DBNGOH010000432.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8309)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8309)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8309
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_798406"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1476)
                     /gene="ftsH"
                     /locus_tag="AC159P_08510"
     CDS             complement(<1..1476)
                     /gene="ftsH"
                     /locus_tag="AC159P_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014267061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HIR3695533.1"
                     /translation="MIFWVLILVGAVMLYHFVVNKPGAGAQQLAYGQLIEEIRNGKIG
                     EMTIKQSEVVSITKDKATTFRTSIEGDKVKGDLMALAVEKDANGALRVPKAQIEAASG
                     GVLWSILAWWGPVLLLLGFWIFMLRQMQSGGNKALSFGKSRAKLLNNQQKRVTFKDVA
                     GVDEAKEELQEIIEFLKEPQKFQKLGGRIPKGVLMMGPPGTGKTLLARAVAGEANVPF
                     FSISGSDFVEMFVGVGASRVRDLFEQGKKNAPCIIFIDEIDAVGRHRGAGLGGGHDER
                     EQTLNQLLVEMDGFESNDGVILMASTNRPDVLDPALLRPGRFDRRVVVSRPDVRGREG
                     ILKVHTRKIPLGDDVDISIVARGTPGFTGADLANLVNEAALNAARYNKKIVMMTDFEL
                     AKDKVLMGAERKSMVITNEEKRVTAYHEAGHTLVGLKVPNADPVHKVTIIPRGMALGV
                     TQQLPEGDRHNYSKDYVLGQIAILMGGRIAEEVFLGNITTGA"
     gene            complement(1715..2227)
                     /locus_tag="AC159P_08515"
     CDS             complement(1715..2227)
                     /locus_tag="AC159P_08515"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012383.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043174 - nucleoside salvage [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HIR3695534.1"
                     /translation="MNERPVELHGPEEIAARVRAVASEIGAAAPGQELTFLGVLEDGY
                     VFLADLLRAVEGPLRTAFLRYEHRHLGGVQDLSFTTEADVRGRDLVLVEGVLDTGVTQ
                     EYIIKQLEARGARSVRLCVLVDKPDGRRAGVLADWRAFETHVDFVVGYGLGFQERWRE
                     LPYLAKLERA"
     gene            complement(2331..3440)
                     /gene="tilS"
                     /locus_tag="AC159P_08520"
     CDS             complement(2331..3440)
                     /gene="tilS"
                     /locus_tag="AC159P_08520"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HIR3695535.1"
                     /translation="MRRRREEEKTKGRASDFARRLLAEWRRRGWASAGEGVVVAVSGG
                     ADSTALLLALDELSTARLLDVCLTVAHLDHGLRGERSEEDARWVEELAKRLGHRVVLE
                     RASVLERAAAGRDNLEQAARRARYEFLAEAARAAGAWAVAAAHTLDDQAETVLLNLLR
                     GSGADGLGGIRAERRLAESAAVLLLRPLVGWARRADTEEYCGARGVEFRADEMNDDER
                     FARVRVRRRLVPLLETFNPRAAEALARTAELVREDAAALEQLAARLLEEAMRAAPEVV
                     DESAAVEDGRAGVGDVVGARPLRVEVLAGAATALRRRALRQWIARGRGDLRRLELAHV
                     VAVERLLAGGRGGRIAELPGGARVERRCGWLFFRR"
     gene            3602..4378
                     /locus_tag="AC159P_08525"
     CDS             3602..4378
                     /locus_tag="AC159P_08525"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3695536.1"
                     /translation="MKSSVYDEGDIHRRYDRARGLPEEATRLWLDRLAPHVPRGPGAA
                     VLDVGCGTGRFTLALAERFDARVYGVEPSAKMIGVAAERVAASRAAPRVSLVRGAAER
                     IPLRDAVAGLALLSMVYHHFADRDSACAELRRVLPVGGRLAVRTATRERVDTFLWHRF
                     FPEARELDLRRLPPSGEPIARLESHGFALTGREVVRQLFARDLAEYHDKISRRALSSL
                     QSITDEAFARGLSELRNYCDTQRTTQPVHEEIDLFIFSAV"
     gene            4477..5475
                     /locus_tag="AC159P_08530"
     CDS             4477..5475
                     /locus_tag="AC159P_08530"
                     /inference="COORDINATES: protein motif:HMM:NF019610.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archaemetzincin"
                     /protein_id="HIR3695537.1"
                     /translation="MRLLSLFIIPALVALPSCGTTRERPEPKPPARVQEEARTPDGLR
                     RALASVRHLHQPLGAPRPGDWLTTSEEPGQTFDEYLQSSPVRATEGRRRIYVQPLGDF
                     DPAQRRVVTLTADFMSRFFGLPVEVRDDLPLSLVPASARRVHPEWGTEQLQAGYISMN
                     ILKPSLPEDAAALVAFTAADLYPGPELNFVFGQASLVDRVGVWSLHRLGDPSANEPSF
                     KLVLLRTMKIAAHETGHMFSVRHCTKYECVMCGSNHINETDRRPIELCPECMAKICWA
                     TDTDPLQRYAGLALFFARHGFPAEQRFCETSIEALRSGPRAAGREHEYGFEIRAII"
     gene            5503..5901
                     /locus_tag="AC159P_08535"
     CDS             5503..5901
                     /locus_tag="AC159P_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR3695538.1"
                     /translation="MEQSIVHIALVVDDYDEAIEFYTEKLDFVLVEDTAQSETKRWVL
                     VAPRGAGGCCLLLAKGVGEEQRSRVGNQTGGRVFLFLKTDDFWRDYENMQARGVNFVR
                     EPKKEEYGTVAVFADLYGNLWDLIEYEGQK"
     gene            6009..7208
                     /locus_tag="AC159P_08540"
     CDS             6009..7208
                     /locus_tag="AC159P_08540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695539.1"
                     /translation="MFSFESGKFSARFASARAHVFDHPQENLAMKTWLLIAIATLALA
                     CVPASRTAVTTAAVTTAQERFEGQWLVEYRPDEQGRIQLTLNYQERREAKDGSKGSGY
                     SNWNSSFRIEPEKLEGLGREQVMSASGTNVRFQLRRDAGALECEGWFREGKGSGHYRF
                     VPNPNFAAELRSRGINGDPTARQLFRMALADAGLALVDELKAQGYETPTFDQLIKMGD
                     HGVRLDYVRGLKEYGYKLGSIDALVKMRDHGVTTSYIKEMRDFGFKNLTAEELVRARD
                     HGVSASFINELEAAGYMNAADSLDGLIRLRDHGVSGSFIREVREEGYTQLPLEQLVRM
                     KDHGVSTNFIKELKALGYANLTVDQLVRLKDHGVNARFISRVKTERGTNPSVEELIRL
                     RNQGYDE"
     gene            complement(7381..8268)
                     /gene="argB"
                     /locus_tag="AC159P_08545"
     CDS             complement(7381..8268)
                     /gene="argB"
                     /locus_tag="AC159P_08545"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: protein motif:HMM:NF012899.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HIR3695540.1"
                     /translation="MSQPRLDLLREALPYIQRFKGKTFVVKLSGKVTEEAENLASLAE
                     ELALLHQVGIRLCVVHGGGKQLTDLAEQMGVAQTIINGRRVTDDATLDMAKMVFAGKI
                     NTDILAALRKRGIHAVGLTGVDGNIVQAVRRPPKDVLNQATGVSEHVDFGHVGDVLEI
                     EDRLLRVLLDHDYMPVVSSLGADAEGRVFNINADTIAAEIAVRLEAEKLIMLSDVDGI
                     YLRPGEQATKLSRLSAEEVTALVRDGVATGGMIPKLQNITDILRRGVRSAHIIKGSLR
                     NALLSEVFTDEGTGTMIVA"
BASE COUNT         1413 a         3084 c         2447 g         1365 t
ORIGIN      
        1 cgcgcccgtc gtgatgttgc cgaggaagac ctcttcggcg atgcgcccgc ccatcaggat
       61 ggcgatctgg ccgaggacgt agtccttcga gtagttgtgg cggtcgccct cgggcaactg
      121 ctgcgtcacg cccagcgcca tcccgcgcgg gatgatcgtc accttgtgga cggggtccgc
      181 gttcggcacc ttgaggccga cgagcgtgtg gcccgcctcg tggtaagcgg tgacgcgctt
      241 ctcctcgttg gtgatgacca tgcttttacg ctcggccccc atcagcacct tgtccttcgc
      301 cagctcgaag tccgtcatca tgacgatctt cttgttgtag cgcgccgcgt tcagcgccgc
      361 ctcgttgaca aggttcgcca aatccgcgcc cgtaaagccc ggcgtgccgc gcgcgacgat
      421 gctgatgtcc acgtcgtcgc ccaaaggaat cttgcgcgtg tggactttga ggatgccttc
      481 gcgcccgcgc acgtccggcc tggagacgac gacgcggcgg tcgaagcgac cgggcctgag
      541 gagcgccggg tcgagcacgt cgggccggtt ggtcgaggcc atcagaatca cgccgtcgtt
      601 cgactcgaag ccgtccatct cgacgagcaa ctggttcaag gtctgctcgc gctcgtcgtg
      661 cccgccgccc aatcctgcgc ctctatgacg accgaccgca tcgatctcgt cgatgaagat
      721 gatgcacggc gcgttcttct tgccctgctc gaagaggtcg cgcacgcgcg aggcgccgac
      781 gccgacgaac atctcgacga agtcggaacc cgagatcgag aagaacggga cgttcgcctc
      841 gcccgcgacg gcgcgcgcca gcagcgtctt gcccgtgccc ggaggcccca tcatcaggac
      901 gcccttcggg atgcgcccgc cgagcttctg gaacttctgc ggctctttca aaaactcgat
      961 gatctcttgc agctcttcct tcgcctcgtc cacgccggcc acgtccttga acgtgacgcg
     1021 cttctgctgg ttgttcagca atttggcgcg cgacttgccg aaactgagag ccttgttgcc
     1081 gcccgactgc atctgccgga gcatgaaaat ccagaacccc aggagcagca gcaccggccc
     1141 ccaccaagcg aggatcgacc agagcacccc gcccgaggcc gcctcgatct gcgccttcgg
     1201 gacgcgcagc gcgccgttcg cgtccttctc gacggcgagc gccatcaggt cgcccttgac
     1261 cttgtcgccc tcgatggacg tgcggaaggt cgtcgccttg tccttcgtga tcgagacgac
     1321 ctccgactgc ttgatcgtca tctcgccgat cttgccgttg cggatctcct cgatcaactg
     1381 cccgtaggcc aattgctgcg cgccggcgcc cggcttgttg acgacgaagt gatagagcat
     1441 caccgcgccg accagtatca gcacccagaa aatcaattgt cttgccgtgg aactcaaatc
     1501 agtctcgcct ccgacgccga tgataaccca tcccggcgcc ttttgtcgct ccccgccgcc
     1561 cttccggccc gcctcccgac cgccgcccgc cgggggccga acgatgcact gcatacggcg
     1621 gtcgcagaag ttttggatgc tttccgggca cccggcgttg cgcttttcgg cccgtgcaaa
     1681 gcgcgtgcaa aaccggcacg gtcttcttac ggactcaggc ccgttcgagt tttgccaggt
     1741 acggcagctc gcgccagcgc tcctgaaacc ccagcccgta accgaccacg aagtccacgt
     1801 gcgtctcgaa cgcgcgccag tccgcgagca cgcccgcgcg ccgcccatcc ggcttatcga
     1861 cgagcacgca cagccgcacg ctccgtgcgc cgcgcgcctc caactgcttg atgatgtact
     1921 cctgcgtgac gcccgtgtcg agcacgccct cgaccagcac gaggtcgcgc ccgcgcacgt
     1981 ccgcctccgt cgtgaaagat aagtcctgaa cgccgcccag atgcctgtgc tcgtagcgga
     2041 ggaaggccgt gcgcagcggg ccttcgacgg cgcgcagcag atcggcgagg aagacgtagc
     2101 cgtcttcgag gactccgagg aaggtcaact cctgtccggg cgcggcggcg ccgatttcgg
     2161 aggccaccgc cctgacgcgt gcggcgatct cttcggggcc gtgtagctct acgggtctct
     2221 cgttcatccg aggctcgcct caaagcgaag accgccggtc gaaaccagcg gtccgttcac
     2281 gacttcgatt gtaaggttcg ggtctacctt tttcaaccca tttctccgcc tcatcgacga
     2341 aagaacagcc acccgcaccg ccgctcgacg cgcgcgccgc ccggcaactc cgcgatcctc
     2401 ccgccgcgcc cgcccgccag cagcctctcg acggcgacca cgtgcgccaa ctccaaccgc
     2461 cgcaagtcgc cgcgcccgcg cgcgatccac tgcctcaacg cgcgcctcct caacgccgtc
     2521 gccgcgcccg ccaacacctc gacccgcaac ggcctcgccc cgacgacatc gccgacaccg
     2581 gctctcccat cttcaacagc ggcgctctca tcaacgactt ccggcgccgc gcgcatcgcc
     2641 tcttcgagca gacgcgcggc caactgttcg agcgccgccg cgtcctcgcg gacgagttcg
     2701 gcggtgcgcg ccagcgcctc cgccgcgcgc gggttgaaag tctccagcag cggcacgagt
     2761 cggcggcgca cgcgcacgcg cgcgaaccgc tcgtcatcat tcatctcgtc cgccctgaac
     2821 tcgacgcccc gcgcgccgca atactcttcc gtgtccgcgc gccgcgccca acccacgagc
     2881 gggcgcaaca gcagcaccgc cgcagactcc gcgagccgcc gctccgccct gatgccgccc
     2941 agcccgtccg ccccgctccc gcgcagcagg ttgagcaaca ccgtctccgc ctgatcgtcc
     3001 aacgtgtgcg ccgcggccac cgcccacgcg cccgccgcgc gcgccgcctc cgccagaaac
     3061 tcgtaacgcg cgcgccgcgc cgcctgctca aggttgtcgc ggccggccgc cgcgcgctcc
     3121 aagaccgacg cgcgctccaa gacgactcga tgaccgagcc gcttcgccaa ctcttcgacc
     3181 caccgcgcgt cctcttcgct ccgctcgccc cgcagcccgt gatccagatg cgcgaccgtc
     3241 agacacacgt cgagcagccg cgctgtcgaa agctcgtcga gcgccaagag cagcgccgtc
     3301 gaatcggccc cgcccgacac ggcgaccacc acgccctcac ccgcagacgc ccacccgcgc
     3361 cgacgccact ccgccagcag ccgccgcgcg aaatcgctcg cacgtccttt cgtcttctcc
     3421 tcctcccgcc gtcgccgcat cttcgaacaa ccagccgttg aaatccgcac gcgcgccgca
     3481 ctactatcgt cccacctcgc gcgcgccgca accctccgcg cgccgaccgc gctaacgacg
     3541 cgagacacgc gcgccgcttc gaacgtccgg cgccgtgtct cgccacttta cctgtcaagc
     3601 gatgaagagt tccgtctacg acgagggcga catccaccgg cgctacgacc gcgcgcgggg
     3661 cctgcccgaa gaggcgacgc gcctctggct cgaccggctc gcgccgcacg tgccgcgcgg
     3721 ccccggcgcc gccgtcctcg acgtgggctg cggcacgggc cgcttcacgc tcgcgctggc
     3781 cgagcgcttc gacgcgcgcg tctatggcgt cgagccttcg gcgaagatga tcggcgtcgc
     3841 cgccgagcgc gtcgccgcgt cccgcgccgc gccgcgcgtc agtctcgtgc gcggcgcggc
     3901 tgagcgcatc cccttgcgcg acgccgtcgc cggcctcgcg ctcctctcga tggtctacca
     3961 ccacttcgcg gacagggaca gcgcgtgtgc cgagctgcgg cgcgtgctgc cggtcggcgg
     4021 tcgcctcgcc gtccgcaccg cgacgcgcga gcgcgtggac accttcctct ggcaccgctt
     4081 cttccccgaa gcgcgcgagc ttgatttacg aagactgccc ccctcgggcg agccgatcgc
     4141 gcgcctcgaa tcacacggct tcgcgttgac cgggcgcgaa gtcgtccgcc agctcttcgc
     4201 ccgcgacctc gccgagtacc acgacaagat cagccgccgc gcgctctcca gccttcaatc
     4261 catcaccgac gaagccttcg cgcgcggcct ctctgagctg cgcaattact gcgacacgca
     4321 acgcaccacg cagcccgtcc acgaagagat agacctcttc atcttctccg ccgtctgact
     4381 cgtcatcgcc gcccgccgaa cctcgcgcgc gagtcgagcg aagagttaaa ctgcggcgcg
     4441 gccacgctcc cacaaaccac ttcgcccgga ggcgctgtgc gacttctctc gctcttcatc
     4501 attccggctc tcgttgccct cccctcttgc ggcacgacgc gcgagcggcc cgagccgaag
     4561 ccgcccgcgc gcgtgcaaga ggaagcgcgg acgcccgacg gtttgcggcg cgcgctcgcc
     4621 tccgtccgcc acctgcacca gccgctcggc gcgccgcggc ccggcgactg gctgaccacc
     4681 agcgaggagc cggggcagac cttcgacgag tacctgcaaa gcagcccggt gcgcgccacc
     4741 gaagggcgcc gccgcatcta cgtccagccg ctcggcgact tcgaccccgc gcagcgccgc
     4801 gtcgtcacgc tcaccgccga cttcatgtcg cgcttcttcg gcctcccggt cgaggtcagg
     4861 gacgacctgc cgctctcgct cgtccccgct tcagcgcgcc gcgtccatcc cgagtggggc
     4921 accgaacagc tacaggccgg ctacatctcg atgaacatcc tcaagccgtc gctgccggaa
     4981 gacgcggcgg cgctcgtcgc cttcaccgcc gcagacctct accccggccc cgagctgaac
     5041 ttcgtcttcg ggcaggcgtc gctcgtggat cgcgtcggcg tctggtcgct ccaccgcctc
     5101 ggcgaccctt cggcgaacga gccgagtttc aaactcgtgc tgctgcgcac gatgaagatc
     5161 gccgcgcacg agaccggcca catgttctcc gtccgccact gcacgaagta cgagtgcgtc
     5221 atgtgcggct cgaaccacat caacgagacc gaccgccgcc ccatcgaact ctgccccgaa
     5281 tgcatggcca aaatctgctg ggccaccgac accgaccccc tccaacgcta cgccggactc
     5341 gccctcttct tcgcgcggca cggcttcccc gccgaacagc gtttctgcga gacctccatc
     5401 gaggctctga gaagcgggcc gcgggccgcg ggccgtgagc acgaatacgg attcgaaatt
     5461 cgggcaataa tatagtcggc tcaccgacgg acaagtaaga cgatggaaca aagtatcgtg
     5521 catattgctt tggtggtaga cgactatgac gaagccatcg aattctatac ggagaaattg
     5581 gatttcgttc tcgtcgaaga cacagcccag agcgaaacga agcgttgggt tctagtcgcc
     5641 ccgagggggg ctggaggatg ctgtctactg ctggcgaaag gggtgggcga agaacaaaga
     5701 agccgcgtcg ggaaccagac gggcgggcgg gtttttctgt tcctgaaaac cgacgacttc
     5761 tggagagact atgaaaacat gcaggcgcgg ggcgtgaatt ttgtcagaga gccgaaaaag
     5821 gaggagtacg gcacggtcgc cgtttttgcg gatttgtacg gcaatctgtg ggatttgatc
     5881 gagtacgaag ggcagaagtg agtgaaccgg tttgactgcc cgaatgcgga atccggaatt
     5941 acgctgcccg ctgccgcaca ctttcctgaa acttccccgc cgccgccgcc acaaacacga
     6001 catcggcggt gttttccttt gagagcggca aattttcggc gcgcttcgcg tccgctcgtg
     6061 ctcacgtctt cgaccacccg caggagaatc tggcgatgaa aacctggctg cttatcgcaa
     6121 tcgcaacact ggctctggcg tgcgtcccgg cgtcgcggac ggcggtcacg accgccgcgg
     6181 tcacgaccgc gcaggaacgc ttcgagggcc agtggctcgt cgagtacagg cccgacgaac
     6241 agggccgtat ccaactgacg ctgaactacc aggagcgccg cgaggcgaag gacggctcga
     6301 aaggctcggg ctacagcaac tggaactcct cgttccgcat cgagccggag aagctcgaag
     6361 gtctcgggcg cgagcaggtg atgtccgcat ccggcaccaa cgtccgcttc cagctccggc
     6421 gcgacgccgg agcgctcgaa tgcgagggct ggttccgcga gggcaaaggc tcgggccact
     6481 accgcttcgt gcccaacccg aacttcgccg cggagctgcg cagccgcggc atcaacggcg
     6541 acccgaccgc gcgccagctc ttccgcatgg cgctcgccga cgccggcctc gccctcgtcg
     6601 atgagctgaa ggcccagggg tacgagacgc ccaccttcga ccagctcatc aagatgggcg
     6661 accacggcgt ccggctcgac tacgtgcgcg gcctcaagga gtacggctac aagctcggct
     6721 ccatcgacgc gctcgtcaag atgcgcgacc acggcgtcac cacctcctac atcaaggaga
     6781 tgcgcgactt cggcttcaag aacctgaccg ccgaggaact cgtccgggcg cgcgaccacg
     6841 gcgtcagcgc cagcttcatc aacgagcttg aggccgccgg ctacatgaac gccgccgact
     6901 cgctcgacgg actcatccgg ctgcgcgacc acggcgtcag cggctccttc atccgcgaag
     6961 tccgcgaaga gggctacaca cagctcccgc tcgaacagct cgtgcggatg aaagaccacg
     7021 gcgtcagcac gaacttcatc aaggagctga aagcactcgg ctacgccaac ctcaccgtcg
     7081 atcagctcgt ccgcctcaaa gaccacggcg tcaacgcccg cttcatcagc cgcgtcaaga
     7141 ccgagcgtgg caccaaccct tcggtcgaag aactcatccg cctgcgcaac cagggatacg
     7201 acgagtaggc agtaggcagt aggcggtagg cagttaaagc ataaagcagg aggcttgaga
     7261 cgttgcatct caagtctcct gctttgttgc ttgaagagcg gagaggccaa ggactttacc
     7321 tcccaagcct cttcgccttt ctgtcttaac tgcctaccgc ctaccgccta ctcctttttc
     7381 tcacgcgacg atcatcgtcc ccgtgccctc gtcggtgaag acttcggaga ggagggcgtt
     7441 gcgcaggctc cctttgatga tgtgtgcgct gcggacgccg cgccgcagga tgtccgtgat
     7501 gttctggagc ttggggatca tgccgccggt ggcgacgccg tcgcggacga gcgccgtcac
     7561 ctcctccgcc gagaggcgcg agagcttcgt cgcctgctcg ccgggcctaa gataaatccc
     7621 gtctacgtcc gagagcatga tgagcttctc ggcttcgagc cggacggcga tctcggcggc
     7681 gatggtgtcg gcgttgatgt tgaagacgcg cccctccgcg tccgccccga gcgaagagac
     7741 gaccggcatg tagtcgtgat ccagcaacac gcgcagcagg cggtcttcga tctccagcac
     7801 gtcgcccacg tgcccgaagt ccacgtgctc gctcacgccc gtcgcctgat tcaacacgtc
     7861 cttcggcggc ctgcgcacgg cctgcacgat gttgccgtcc acgcccgtca gcccgaccgc
     7921 gtgaatcccg cgcttccgca gggcggcgag gatgtccgtg ttgatcttcc cggcgaagac
     7981 catcttcgcc atgtcgagtg tcgcgtcgtc cgtcacccgc cgcccgttga tgatcgtctg
     8041 cgcgacgccc atctgctcgg ccagatccgt caactgcttg cccccgccgt gcaccacgca
     8101 caggcggatg ccgacctgat gcagcagcgc caactcctcg gccagtgacg ccaggttctc
     8161 cgcctcctcc gtcaccttcc ccgacagctt caccacgaag gtcttgccct tgaaccgctg
     8221 gatgtacggc aaagcctcgc gcaacagatc gagccgcggc tgactcacgc tttcacctcc
     8281 ggtgaaagaa ggcaaaaggc aaaaggcaa
//
LOCUS       DBNGOH010000433         3854 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_433061, whole genome shotgun sequence.
ACCESSION   DBNGOH010000433 DBNGOH010000000
VERSION     DBNGOH010000433.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3854)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3854)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3854
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_433061"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            40..420
                     /locus_tag="AC159P_08550"
     CDS             40..420
                     /locus_tag="AC159P_08550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695541.1"
                     /translation="MPLADQPDHELGIAEVSGTQKSPDPLWDDSKITYWGVTDLLGDQ
                     GTQRGYYHNVHPDGGREWGTFEGRVAATGGAVTVEGTWEMAGGDGEYRGITGGGKFKT
                     AMKSEAELECSWDGNYELAKAQTG"
     gene            638..1567
                     /locus_tag="AC159P_08555"
     CDS             638..1567
                     /locus_tag="AC159P_08555"
                     /inference="COORDINATES: protein motif:HMM:NF024627.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HIR3695542.1"
                     /translation="MKVHLTYMNGIPRLAATAFAAVALVTVSLVWGGATVSANHPVYV
                     EGNCLNPPAGNPGPVTPGTCGDYDGDGRIGTAEDADGDRVFGTITAALGGGTGANQNG
                     RVVIVTSGTFAEVVNITGANGNVQLEAAPGVDANIDAVLQGDPGSGARQNAPGIIVNA
                     PADRFITIRNIVSRNWATGIDVRGFSRVLIDRCRVDNNTNFGILVQGTAKVKINDTQV
                     TATGFRVGATGDFPRANRPDPGVGIEFQDDSSGLICDSCVVGNFGAGIRNGTRDNGKG
                     FGNSSVQLQNVCAFDNNPDYVGVKARTGSGKDR"
     gene            2128..2457
                     /locus_tag="AC159P_08560"
     CDS             2128..2457
                     /locus_tag="AC159P_08560"
                     /EC_number="5.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019013.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HIR3695543.1"
                     /translation="MAEDVLFTVMATGQEHRGPDGVRQMLNYFYHVAFDATAETTNTI
                     FADGKAVVEGDFVGRHVGEFAGIPATYKHVRVPMCVVYDLENDQIKRGRVYFEMPVLL
                     RQLGIGS"
     gene            2775..3251
                     /locus_tag="AC159P_08565"
     CDS             2775..3251
                     /locus_tag="AC159P_08565"
                     /inference="COORDINATES: protein motif:HMM:NF024954.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR3695544.1"
                     /translation="MRQPRAADIRRLEAELEDFPQPRQRRRAEAILLHAAGLEATAIA
                     QTLAAHPNTIRADLHAFDRDGLRAVQAAGSRPAPRGFTPGQVAEVCHLAETAPYELGL
                     PWGRWSLAKLRDYLLKERVCRHLSREHLRRLLRKGGCTLPASSARSSVQTRSGAPS"
     gene            3299..3607
                     /locus_tag="AC159P_08570"
     CDS             3299..3607
                     /locus_tag="AC159P_08570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695545.1"
                     /translation="MFFDVKPVFVKAYGGRRWTSAKRLVLPKQQRTRGKFYLFLLYDA
                     RAGRARWRYLPGKNSEYVCQFMRQVRRWYPTQAVWVGLDQDRPHPRISRATRRVRCGG
                     "
     gene            3595..3819
                     /locus_tag="AC159P_08575"
     CDS             3595..3819
                     /locus_tag="AC159P_08575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695546.1"
                     /translation="MRRLKLRWVSLPKRRPDDNPVETVFSDIQQMVLDNSNDVDAAAT
                     QRRITAHLRRRNRRRGRYLKIKYLGDTHKT"
BASE COUNT          730 a         1236 c         1190 g          698 t
ORIGIN      
        1 acaggaactt tttcgggaac aatcacgaag cagtcggcca tgccgctagc cgaccaaccc
       61 gaccacgagc tgggcatcgc cgaggtgagc ggcacgcaga aatcgcccga cccgctgtgg
      121 gacgactcga agatcaccta ctggggcgtg accgacttgc tcggcgacca gggcacgcag
      181 cgcggctact accacaacgt ccaccccgac gggggccgcg agtggggcac gttcgagggc
      241 cgggtcgccg cgacgggcgg ggcggtgacc gtcgagggca cctgggaaat ggccggcggc
      301 gacggcgagt accgcggcat caccggcggc ggcaagttca agacggcgat gaagtctgag
      361 gccgaactcg agtgcagctg ggatggtaac tacgagctgg ctaaggcgca gacgggctga
      421 acaacagggc gcggtcaggc caaatagagg cgcagaggcg ggcgcatcat cggctcgccc
      481 ctgccgccct tgatagctgc gcatcaaatg tgcagatgaa cccgcggcgt gagatggctt
      541 tcgcctagat gaggcgcaac gctttttagc gcaggcacac catctaacaa cggccttatg
      601 tgtgcgtctt acgcccctct cacttcagga ggtacatatg aaagttcacc tgacttatat
      661 gaacggcatc ccgcgcctag ccgccacggc ttttgcggcg gtggcgctcg taacggtgtc
      721 cctcgtctgg ggcggcgcca ccgtctcggc caaccacccg gtgtacgtcg agggcaactg
      781 cctcaaccct ccggctggta atccggggcc ggtcacgccc ggcacgtgcg gcgactacga
      841 cggcgacggc cggatcggca cggcggaaga cgccgacggc gaccgggtgt tcggcaccat
      901 cactgccgcg ctcggcggcg gcacgggcgc taaccagaac gggcgcgtcg tcatcgtcac
      961 gtccggcacc ttcgccgagg tcgtcaacat caccggcgct aacggcaacg tccaactcga
     1021 ggccgcgccg ggcgtcgacg cgaacatcga cgccgtgctg cagggcgacc ccgggagcgg
     1081 cgcccgccag aacgcgcccg gcatcatcgt caacgcgccc gccgaccgct tcatcacgat
     1141 ccgcaacatc gtcagccgca actgggccac cggcatcgac gtgcgaggct tctcccgcgt
     1201 gctcatcgac cgctgccgcg tcgataacaa taccaacttc ggcatcctgg ttcagggcac
     1261 cgcaaaagtt aagatcaacg acacgcaggt gaccgcgacc gggttccgcg tcggggcgac
     1321 gggcgacttc ccgcgggcca acaggccgga cccgggcgtc ggtatcgaat tccaggacga
     1381 ctcatcgggt ttgatttgtg actcgtgcgt cgtcggcaac ttcggggcgg ggatcaggaa
     1441 cggcacgaga gacaacggga agggcttcgg caactcgtcg gtccaactgc agaacgtgtg
     1501 cgccttcgac aacaacccgg actacgtggg cgtcaaagca cggacgggtt ccggcaagga
     1561 ccgctagggg ccgagaggaa tgcttcctcg gtagagtgcc gccccctcat caaaggcggg
     1621 cggcattcgt tttatctacc ccgtcacact ccccaccgtg ggttgatttc ggcatcgggt
     1681 gcgcctcgtg gccacagaat cgcgccgtac gctaatttcg gcatctcggc ttagagagcc
     1741 atgcctcgta agtaccccct acgtcacggc gcgactctgc gcgattggtg tttcccccgc
     1801 cccgaattct aaatcaccgt ctcacgtttg gagtaggaaa cgtcaacacc tctcccggcg
     1861 gcctgtgcat tcctcggctg acatgaaaac ttccgtcgaa cttttctccg tgatcttctg
     1921 gaaagcctca tcctcgattc cggatttcat cgtaaagcta tctctgttgt aaattgtatg
     1981 tcgcttacat atggcggttc gactcccgcg tcggggcgcc cattccagtt acattggatt
     2041 ctttggggga gatatcctat gtcggtcgat agtacacgcg aggtgatgac caggtatttc
     2101 aactccggcc actcggacgt gagcatgatg gcagaggacg tcctcttcac cgtcatggcg
     2161 accgggcagg agcaccgcgg ccccgacggc gtgcggcaga tgctcaacta cttctaccac
     2221 gtcgccttcg acgccaccgc ggagaccacc aacacgatct ttgccgacgg gaaggcggtc
     2281 gttgaaggag actttgtggg taggcacgtc ggcgagtttg cgggtatccc ggccacgtat
     2341 aagcacgtac gcgtgccgat gtgcgtggtc tatgacctgg aaaatgacca gatcaagcgc
     2401 gggcgtgtct atttcgagat gcctgttttg ctccgacaac ttgggatcgg tagttgatta
     2461 gtaacgccga tgattcccaa gtgcttcata ggagggcgct gctccacact acgttgaata
     2521 attcatccgc ttttctcttg cgttaatctt ttggcttgcg ctttcgataa tcagcggagg
     2581 ctagccgtac cgcctcacca cgcttccggc acggctcaca gaaactcata cggttctgtc
     2641 tcggctacct cgcggggacg taggtgttaa ccgtactgtc acatctgaga tgtgagtatt
     2701 tgtcacatcg gttttgttag aatcgttgaa ccacgctatc tgtcggaggt tcaacgatgc
     2761 cacgttcttt gaaagtgcga cagccacgag cggctgacat ccggcgactc gaagcggagt
     2821 tggaggactt ccctcaaccc cggcaacggc ggcgtgctga agccatcctg ctccacgccg
     2881 ccgggctgga ggcgaccgcc attgcacaga ccctcgccgc ccaccccaat accatccgcg
     2941 ctgacttgca cgccttcgac cgcgacggcc tgcgggccgt gcaagccgcc ggtagccgac
     3001 ccgcgccgcg cggcttcacg ccggggcagg tcgccgaggt ctgccacctc gccgagactg
     3061 cgccctacga gttgggtctg ccctgggggc ggtggtcgct ggcgaagctg cgcgactacc
     3121 tgctcaaaga gcgcgtctgc cgccatctca gccgtgagca cctgcgccgc ctgctccgga
     3181 aagggggctg cactttgccc gcgtccagcg caaggtcatc agttcagacc cgcagcggcg
     3241 cgccatcctg agccgcctgc gcctgatctg gcggcactta tccgaagacg gcgcgctctt
     3301 gttcttcgat gtcaagccgg tcttcgtcaa agcttacggc gggcggcgct ggacttcggc
     3361 caagcggctg gtgctgccca agcagcagcg gacgcgcggc aagttctacc tcttcctgct
     3421 ctacgacgcg cgggccgggc gggcgcgctg gcgctacctg ccgggcaaga acagcgagta
     3481 cgtctgccag ttcatgcggc aggtgcggcg ctggtatccg actcaggccg tgtgggtcgg
     3541 gcttgaccaa gaccgcccgc acccgcgcat ctcgcgggcg acgcggcgtg tgagatgcgg
     3601 cggctgaagc tgcgatgggt gagcctgccc aagcgccgcc ctgacgacaa cccggtggag
     3661 acggtcttca gcgacatcca acagatggtt ctggacaaca gcaacgacgt tgacgccgcg
     3721 gccacgcagc gccgcatcac agctcactta cggcggcgca accgccgccg gggtcgatat
     3781 ctcaagatca agtatctggg agatactcac aaaacgtagg tgacaaatag tcacatcagc
     3841 aatgtgacag tacg
//
LOCUS       DBNGOH010000434         3971 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2449620, whole genome shotgun sequence.
ACCESSION   DBNGOH010000434 DBNGOH010000000
VERSION     DBNGOH010000434.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3971)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3971)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3971
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2449620"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..917)
                     /locus_tag="AC159P_08580"
     CDS             complement(<1..917)
                     /locus_tag="AC159P_08580"
                     /inference="COORDINATES: protein motif:HMM:NF019335.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HIR3695547.1"
                     /translation="MRGARNYWLGGGHVWRVCGVVLLLLACGGFSVGAQTVTGRISGT
                     VKDATGAVVPGAAVTVTNAANNLARTATTDEDGFFTVTNLPAGTYAVLAEREGFKKAL
                     QSEVSLVADARLTVDLTLETGAVAETVEVTGSVGETVNTTSGEVARVIDSQQVQNLAL
                     NGRNYIQLLSLVPGVALTNDDQLELSTSLATGNQSINGNRGQTNNMTVDGGTNLQSGS
                     NASQINNVGIDFIQEVKIQTSNFSAEYGRNSGASINVVTRSGGNQFHGSLFEFLRNDK
                     LDANNFFNNARGRFTDNTGARAPDVIVPAG"
     gene            complement(1079..2305)
                     /locus_tag="AC159P_08585"
     CDS             complement(1079..2305)
                     /locus_tag="AC159P_08585"
                     /inference="COORDINATES: protein motif:HMM:NF012692.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HIR3695548.1"
                     /translation="MRKIDTRDFHVATRSTSREINRRIALNLIREHQPISRADLARRM
                     SVTRGVASVLVQELIEQGLIYEGATGEAARGRKPTFLHIRTQDRLAVAVDVRFSKTYL
                     MLCDFAGRQIALEISDTIFTIPEFVKDLAARVRRMLKTQQVEGRCEGIGVVVPGMVDQ
                     RTGRILNAPTLGWREVDIRDRLAAATGLPVQLENSARACSLAQLWLGRGESAGPQSFV
                     YVSVSDGVGVSVVVNGELVRGRDHIAGEFGHMPLSLDGPRCMCGMTGCWEAYISNLAT
                     LSRYFGRDLSKLGPKAPRDAGQDSFSVTDLASRARGGDAKAVAAIQATARFLGLGLGT
                     IVNGVNPDSIYLSGEIMSAWDLIEAAVREGLAERSLTEAAAHTPLRVVPAQEQPRLRG
                     AAALIAAPVFAAPRVA"
     gene            complement(2577..3236)
                     /locus_tag="AC159P_08590"
     CDS             complement(2577..3236)
                     /locus_tag="AC159P_08590"
                     /inference="COORDINATES: protein motif:HMM:NF018712.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1326 domain-containing protein"
                     /protein_id="HIR3695549.1"
                     /translation="MRGLLCLVLAMLALCGGAFSVSRAEANRLEGEYVEARTASVFAG
                     ACHYNGELTTAGREAVLAWHVTAGSWDGVDLAGVRALAIVGADANLSDAQAARRAELI
                     IDESASYKQAVAFTNAVKSKYGATLGRVAVVRSGPVSFRHESKAYTVNAPGLAAMNVE
                     AMPDDLCCTMPQLVWYEPLVPLARRKVGYTAKASYAGGTIGDAWQRTGENSAFYGSFS
                     F"
     gene            complement(3302..3574)
                     /locus_tag="AC159P_08595"
     CDS             complement(3302..3574)
                     /locus_tag="AC159P_08595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695550.1"
                     /translation="MADQLNYHEEAAAAGSTDAGATAQSSFCGSLRSKKFFMLDGLAT
                     DASQYLDASNHCWCRETQQVVGPDGSRVNPSRCVPGRACYSSSLEG"
     gene            complement(3601..3909)
                     /locus_tag="AC159P_08600"
     CDS             complement(3601..3909)
                     /locus_tag="AC159P_08600"
                     /inference="COORDINATES: protein motif:HMM:NF015415.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695551.1"
                     /translation="MKFKVRDSLDFALASVAAAVEVAANKTVRQARVVLGGVAPVPWR
                     APKAEGYLVGKTLTKDVLAEAARLALDGAAPLEKNAYKVPLTETLVRRALAKAGGVAA
                     "
BASE COUNT          648 a         1376 c         1280 g          667 t
ORIGIN      
        1 ccggccggga cgatgacgtc gggcgctctg gcgccggtgt tgtcggtgaa gcggcccctg
       61 gcgttgttga agaagttgtt ggcgtcgagc ttgtcgttgc gcagaaactc gaagaggctg
      121 ccgtggaact ggttgccgcc cgagcgggtg acgacgttga tcgaggcgcc ggagttgcgc
      181 ccgtactcgg ccgagaagtt ggaggtctgg atcttgacct cctgaatgaa gtcgatgccg
      241 acgttgttga tctggctcgc gttgctgccc gactgcaagt tggtgccgcc gtccaccgtc
      301 atgttgttgg tctggccgcg gttcccgttg atggactggt tgcccgtggc gagactcgtg
      361 ctcagctcca actggtcgtc gttcgtcagg gccacgcccg gcaccagcga gagcaactgg
      421 atgtagttgc gaccgttcag cgcgaggttc tgcacctgct ggctgtcgat cacgcgcgcc
      481 acctcgcccg acgtggtgtt caccgtctcg cccaccgagc cggtgacctc gaccgtctcg
      541 gcgaccgcgc cggtttcgag cgtgagatcg accgtcaggc gcgcgtcggc gacgaggctc
      601 acctccgact gcaatgcctt cttgaaccct tcgcgctcgg ccagcacggc gtaggtgcct
      661 gcgggcaggt tcgtcacggt gaagaacccg tcctcgtcgg tggtcgccgt gcgcgcgagg
      721 ttgttcgcgg cgttcgtgac ggtgacggcg gcgccgggga cgaccgcgcc cgtcgcgtct
      781 ttcaccgtgc cggagatgcg cccggtgacg gtctgcgccc cgacagaaaa gccgccgcac
      841 gccagcagca acaagacgac accgcagacg cgccagacgt ggccgccgcc caaccagtag
      901 ttccgtgcac ctcgcatcac actcacctcc gaatgaagaa tcgagccaca cccgcgggca
      961 acactccgcc gccgagcagc agcgcacgcg cttgcccctt cccgaactat tcgtttttcc
     1021 cctcgacgtt cccctgaagc ggggaagaac ctgcttggaa cttgcgcggg gccgtgctct
     1081 aggccacgcg cggcgcggcg aagaccggcg ccgcgatgag cgccgcggcc ccgcgcaggc
     1141 gcggctgctc ctgcgccggc acgacgcgca ggggcgtgtg cgcggcggcc tccgtcagcg
     1201 agcgctcggc cagcccctcg cgcaccgccg cctcgatcaa atcccacgcc gacatgatct
     1261 cgcccgacag gtagatgctg tccgggttga caccgttgac gatggttccc aacccgaggc
     1321 ccagaaagcg cgccgtggcc tgaatcgcgg cgacggcctt cgcgtcgccg ccgcgcgcgc
     1381 gcgaggcgag gtcggtcacc gaaaaagagt cctgcccggc gtcgcgcggg gcttttggcc
     1441 cgagtttgga caaatcacgc ccgaaatagc gcgacagggt agcgaggttc gagatgtagg
     1501 cctcccagca ccccgtcatc ccgcacatgc agcgcggccc gtcgaggctc aggggcatgt
     1561 gcccgaactc gcccgcgatg tggtcgcgcc cgcgcaccag ctcgccgttc acgaccacgc
     1621 tgacgccgac gccatccgac acgctcacgt agacgaagct ctgcggcccc gcgctctcgc
     1681 cgcgcccgag ccacaactgc gccagcgaac acgcgcgcgc cgagttctcc aactgcaccg
     1741 gcaaaccggt cgccgcggcc agccggtcgc ggatgtccac ctcgcgccag cccagggtcg
     1801 gcgcgttcag gatgcgcccc gtgcgctgat cgaccatgcc ggggacgacc acgccgatcc
     1861 cctcgcagcg gccctcaacc tgctgtgttt tcagcattct gcgcacgcgc gcggccaaat
     1921 ccttgacgaa ctcggggatg gtgaagatgg tgtccgagat ttcgagcgcg atctgtcggc
     1981 ccgcgaaatc gcacagcatc aggtaggtct tgctgaagcg cacatcaacc gccaccgcca
     2041 gtcgatcctg cgtccgtatg tgcaggaaag tgggtttgcg accgcgcgcc gcctcgcccg
     2101 tcgcgccctc gtagatcagt ccctgctcga tcaactcctg caccagcacg ctcgccacgc
     2161 cgcgcgtcac gctcatgcgc cgcgccaggt cggcgcgcga gatgggctgg tgctcgcgga
     2221 tcaggttgag tgcgatgcgc cggttgatct cgcgcgaagt ggagcgcgtg gcgacgtgaa
     2281 agtctctggt gtcgatttta cgcatggggc gagccggaca tttgttccga gtcgggacaa
     2341 tatcccctcg taaaatttct gtcaagactt ttttttccga aaagaacccc ggaacgccgg
     2401 agcagcggaa ggcgcgaccg ccagtgaaag gcaaagtaac agttgaaagg cgaaagtgcg
     2461 gatgacgaaa agcagcgtgc ctctcatcat caattcgatg agaggcacgc tgctcccagt
     2521 cttccctttc gcctttttac ttccgcggaa gtcggctgct gccgtcttcg accgtgctag
     2581 aaggagaagc tcccgtagaa ggcgctgttc tcgcccgtcc gctgccacgc gtcgccgatt
     2641 gtgccgccgg cgtacgaagc cttcgcggtg tagccgacct tgcgacgggc gagcggcacg
     2701 agcggctcgt accaaacgag ctgcggcatc gtgcagcaca ggtcgtcggg catggcctcg
     2761 acgttcatcg cggccagccc cggcgcgttg accgtgtagg ctttgctctc gtgcctgaag
     2821 ctgacggggc cgctgcgaac cacggccacg cgaccgagcg tcgcgccgta cttgctcttg
     2881 accgcgttgg tgaaggcgac ggcctgcttg taggaagcgg attcgtcgat aatcagctcg
     2941 gcgcgccgcg cggcctgtgc gtccgagagg ttggcgtcgg cgccgacgat ggcgagcgcg
     3001 cgcacgcccg cgaggtcaac gccgtcccaa ctgcccgcgg tgacgtgcca ggcgagcacg
     3061 gcctctcggc ccgcggtcgt cagctcgccg ttgtagtggc acgcgcccgc gaagacgctc
     3121 gccgtgcgcg cctcgacata ctcgccttcg agcctgttcg cttccgcgcg cgacacggag
     3181 aacgcgccgc cgcagagggc caacatcgcc aacaccagac acaacagacc acgcatcaag
     3241 acacctccgt tcaaaacccg cgtcaatact ttacagagta tggctcacgt cccgtccgcc
     3301 ctcaaccttc gagcgaagag ctgtagcagg cgcggcccgg cacgcagcgc gaagggttga
     3361 cgcggctgcc gtcggggccg acgacctgct gcgtctcgcg gcaccagcag tggttgctgg
     3421 cgtcgaggta ctggctcgcg tcggtcgcca gaccgtcgag catgaagaac ttcttcgaac
     3481 gcagcgagcc gcagaaagaa ctctgcgcgg tcgcgcccgc gtccgtcgaa cccgccgcgg
     3541 ctgcctcttc atgataattc aactgatcgg ccatcgcacc ctccgtcgca tccttcgtct
     3601 ttaggccgcc acgccgccgg ccttcgccag cgcgcggcgc acgagcgtct cggtcaaagg
     3661 aactttgtag gcgttcttct caagcggcgc ggcgccgtcg agcgcgaggc gcgcggcctc
     3721 cgccagcacg tccttcgtca gggtcttgcc gacgagatag ccttcggcct tcggcgcgcg
     3781 ccacggcacg ggcgcgacgc cgccgagcac gacgcgcgcc tgccgcacgg tcttgttcgc
     3841 ggcgacctcg accgccgccg cgaccgaagc gagcgcgaag tcgagcgagt cgcgcacttt
     3901 gaatttcaag taagtctagc gcgcggcgaa ggccgacgcg ggcacgtgaa tctccgtgat
     3961 gatctcttcg t
//
LOCUS       DBNGOH010000435         5519 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2998885, whole genome shotgun sequence.
ACCESSION   DBNGOH010000435 DBNGOH010000000
VERSION     DBNGOH010000435.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5519)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5519)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5519
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2998885"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(251..673)
                     /locus_tag="AC159P_08605"
                     /pseudo
     CDS             complement(251..673)
                     /locus_tag="AC159P_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214492.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
     gene            1023..1172
                     /locus_tag="AC159P_08610"
     CDS             1023..1172
                     /locus_tag="AC159P_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008478596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lmo0937 family membrane protein"
                     /protein_id="HIR3695552.1"
                     /translation="MLWTILVVLLILWLLGAIGGVGGSLIHLLLVVALVILVINLLTG
                     RRTVV"
     gene            complement(1353..1574)
                     /gene="infA"
                     /locus_tag="AC159P_08615"
     CDS             complement(1353..1574)
                     /gene="infA"
                     /locus_tag="AC159P_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HIR3695553.1"
                     /translation="MAKEDLIPAEGVVVDKLPNAFFKVKLDGSDHVVLAQISGKMRKN
                     YIRILEGDRVSVELSPYDLTRGRITYRFK"
     gene            complement(1664..2893)
                     /locus_tag="AC159P_08620"
     CDS             complement(1664..2893)
                     /locus_tag="AC159P_08620"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018290022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIR3695554.1"
                     /translation="MNFSQLGLDAPLVGACESLGYTEPTPIQQEAIPVVLGGSDLIGC
                     AETGTGKTAAFLLPLIQRMSRRARPGVRLLVLAPTRELATQIEASYKSLAPKGSPRCA
                     LVIGGAAMKRQTDNLKKGAGVIVATPGRLLDQMERGTIDLARIEALVLDEADRMLDMG
                     FWPSIRRVLAVLPSERQTLLFSATMSPEIERLARSTMKSPALVEVNRRGSAAEMVDQM
                     AYPVATESKTALLIDLLEKEREQENFDRVLVFTRTRRGAERLSHILEARDHKVNRIHA
                     DRTQPQRDAALRGFREGHTRVLVATDIAARGIDVDAVSHVINYDVPEAPEDYVHRIGR
                     TGRAGNRGRAITLVTPVDESSMKAIERLTGQPVERVHVPGFSAAPALAQTASKTFASV
                     RHRAAVGRRSFRPRRSR"
     gene            complement(3038..3442)
                     /locus_tag="AC159P_08625"
     CDS             complement(3038..3442)
                     /locus_tag="AC159P_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010044502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA];
                     GO_process: GO:0006397 - mRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA recognition motif domain-containing protein"
                     /protein_id="HIR3695555.1"
                     /translation="MSMKLYVGNLSFQTSSEDLQELFAKAGTVESATVVEDRETGRSR
                     GFGFVEMATNEEGQAAIQQFNGKDFNGRNLTVNEARPREDRGGRGGGGGGRGGYGGGG
                     GGRGGGYGGGGGRGGGGGYGGGGGGGNREPRW"
     gene            4290..>5519
                     /locus_tag="AC159P_08630"
     CDS             4290..>5519
                     /locus_tag="AC159P_08630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695556.1"
                     /translation="MERLVQILFGHERAVFTNGQFGFGLRPHPAVLVAGALLFCAFIY
                     FVYVRPRARLRGPKLAALISLRVALFALLVFLLLRPVVVVPSVVPRSTSVALVADDSR
                     SMQIADAPGRRTRLEAVRAALFSAETKFLPRLQEKFRTDLYAFSGELGALGGGGEELF
                     GEGAASDLGGAVSEAARRAAGVPLSAVVLVSDGAANVPRDLGAVLRELRAKNLPVYTV
                     GVGSAERPTDAELVRVQMPRRVLVGSSVNVEAFVRLSGYGPTKVLIAVSEDGRAVKTE
                     EFNLRGGETEAVRLELTAAIAGVHRYTFEITALDGEMTLENNGREALVEVIEGPLRVL
                     YVEGEPRWEHGKMRSAVTRNEKNVELVSILRTGENKLYRQGISGEQELAAGFPATEEE
                     LFAYDGLMLGSVEAGFFT"
BASE COUNT          853 a         1717 c         1910 g         1039 t
ORIGIN      
        1 ggggccgtcg aagtacgcga ccgcccgccc gccgtgctcg cgttcgagcg cagggaagtg
       61 cgacctgatc tccgaagtgg agagaatgtc tgtcgccgac tgcgttgctg ttttgttcat
      121 attcagaagt gtaggcggta ggcagtaaga agagccgcgt gacttgatgt tcgagacttg
      181 gaatctcacg ccttgaattg caaattcaga gtcgagaatt tctgtctgcc tactgcccac
      241 cgcctactgc ctactgtccg atgattttga cgaggactcg tttggcgcgg cgcccgtcga
      301 actcgccgta gaagatttgt tcccaggggc cgaggtcgag gcggccttcg gtgacggcga
      361 cgacgacttc gcggcccatg atctggcgct tgtggtgcgc gtcgccgttg tcctcgccgg
      421 tgcggttgtg tcggtacgcc tcgggcgagg ggtcgaaggg cgcgagtcgt tcgagccagc
      481 gtttgtagtc ttcgtgcagc cccggctagt cgtcgttgat gaagacgctg gccgtgattt
      541 gcatcgcgtt gacgaggcag aggccctcgc gcacgcccgc cgagcggact tccgcggcga
      601 cctcgtcggt gatgcggacg aagcccatcc gctcggggac gttgaaggtc aggtacttcg
      661 tgtgtgattt cacgccggct cctctcggtc gggtcggagc acgtatctta aaggcaaccg
      721 tgaccgaagt tgaagtccgg acgggagggt cggcgttttc gcatcgtatc cgaagcggcg
      781 gaggactgaa tgagaacaac aagggacgcg gcgaatggct tgtttcagtg caagcgtcgg
      841 ggcgcgcggt cgtgccaatt tgacggcttg agatggcgtg cggccttcaa gtctttcagg
      901 cgagaaggtt tacgtttctt cgcggcgggc gcgggcgcgg gcggcatgga ggttgccaaa
      961 tgtgtgggcg ggcagttagt taggctcaag cttcggggga cgtgaagaaa aggagtataa
     1021 atatgctttg gacgattctt gtcgtgttac ttattctgtg gttgctcggt gcgatcggcg
     1081 gcgtcggcgg tagcttgatt cacctgctgc tcgtcgtggc gctcgtgatc ctcgtcatca
     1141 acctgttgac gggtcgccgg acggtcgtct aggcgggcgc gagctgactg taggggaagg
     1201 gtgagttttc agtggacgga gcgtaaggtt agaaactccg gctctgaacc ctgaggggtt
     1261 cgccggtttc ttgcaaagcc tcggtagcac taccggagca cgtccacaag gggcggcgtc
     1321 gcttcaagcg aagcggcgcc gcttcgcttt gtttatttga accggtaggt gatccgcccg
     1381 cgggtcaggt cgtagggcga cagctcgacg ctgacgcggt cgccttccag gatgcggatg
     1441 tagttcttgc gcatcttgcc cgaaatctgg gcgagtacga cgtgatcgct gccgtcgagc
     1501 ttaactttga aaaaggcgtt cggcagctta tcgacgacga cgccctccgc ggggattaaa
     1561 tcttctttcg ccaagcctct ctctcctgat tcatagaatg agatgtttga gccgcgcgct
     1621 ttctgagggt gcgcggcccg ccaatggtgt ttacgtaacg cccttagcgc gagcgccggg
     1681 ggcggaagga gcgtcgcccg acggcggcgc ggtgccggac ggaggcgaac gtcttggatg
     1741 cggtttgcgc cagcgcgggt gccgcgctga agccggggac gtggacgcgc tcgaccggct
     1801 ggccggtcag gcgctcgatg gccttcatcg aggactcgtc cacgggcgtg accagcgtga
     1861 tcgcgcgccc gcggttgccc gctcttccgg tgcgaccgat gcggtgcacg tagtcttcgg
     1921 gcgcctcggg cacgtcgtag ttgatgacgt gcgagacggc atcgacgtcg atgccgcggg
     1981 cggcgatgtc ggttgccacg aggacgcgcg tgtggccctc gcggaagccg cgcagggcgg
     2041 cgtcgcgctg cggctgcgtg cggtcggcgt ggatgcggtt caccttgtgg tcgcgcgctt
     2101 cgaggatgtg cgagagcctt tcggccccgc gccgcgtgcg cgtgaagacg agcacgcggt
     2161 cgaagttctc ctgctcgcgc tccttctcca gcagatcgat gagcagggcc gtcttcgact
     2221 cggtcgccac ggggtaggcc atttgatcga ccatctccgc ggcgctaccg cgccggttga
     2281 cctcgacgag cgcgggcgac ttcatcgtcg agcgcgcgag gcgttcgatc tcgggcgaca
     2341 tggtcgccga gaagaggagc gtctgccgct ccgaggggag caccgccagc acgcgccgga
     2401 tcgagggcca gaagcccatg tcgagcatgc ggtcggcctc gtcgaggacg agcgcttcga
     2461 tgcgcgccag atcgatcgtg ccgcgctcca tctggtcgag caggcggccc ggagtcgcca
     2521 cgatgacgcc cgcgcccttc ttcaggttgt cggtctgccg cttcatcgcg gcgccgccga
     2581 tgacgagcgc gcagcgcggg ctgcccttcg gcgcgagcga cttgtagctc gcttcgatct
     2641 gcgtcgccag ttcgcgcgtc ggcgcgagca cgagcaggcg cacgccgggg cgcgcgcgcc
     2701 gcgacatgcg ttggattaaa gggagcagga acgcggcggt cttgccggtg ccggtctcgg
     2761 cgcagccgat caggtcgctg ccgccgagga cgaccgggat cgcctcctgc tggatgggtg
     2821 tcggttcggt gtatccgaga gactcgcacg cgccgaccag cggtgcatcg agtcccagtt
     2881 gtgagaaatt catgaacatc cttcggcgct gccgtggcgt aacgttcgcc cgcgcgccct
     2941 tctgctgagt gaaaatgtcg aagcgccccc ggcggcgttc ttgtcgtcgt cggaggcgct
     3001 ttgcttcaaa gagtcaccgg cgagcctggc tggtggatta ccagcgcggc tcgcggttgc
     3061 cgccgccgcc accaccgccg tagccgccgc caccgccgcg accaccgccg ccgccgtaac
     3121 cgccgccacg accgccgccg ccgccgccgt agccgccgcg accaccgccg ccaccgccgc
     3181 gaccgccgcg gtcttcacgc gggcgggctt cgttgacggt caggttgcga ccgttgaaat
     3241 ccttcccgtt gaactgctgg atggccgcct ggccctcctc gttcgtagcc atctcgacga
     3301 agccgaagcc gcgcgaacgg cccgtctcgc ggtcttcgac gaccgtcgcc gactcgaccg
     3361 tgcccgcctt cgcgaacagt tcctgcaaat cttcgctcga tgtctggaac gagagattac
     3421 cgacgtagag tttcattgac atggacgtgt accttctcct tgcgcaggag ttctatgaga
     3481 agctgcgaat ttgtggcttc gatgtaacgg tgggcagcga aggcgcgcgt tcgggtgctc
     3541 tttgaaagga gccgcttctg aagttgcaat caacctactc tcgaaccatc ccaaacgccg
     3601 ccgcgaagac tgctaagcat gtggcgcatc accgttgacg caagagagaa ggagtaaccg
     3661 agagctatga tgtcataaca ggccaacaag ttgcaagccc cacggcggcg ggcggcaggc
     3721 agcgggcagt caagacaaaa tgccgaagag ccttgagaga taagtcccgt gtctcttctc
     3781 tattccgacc cctgttctgc tgccttgact gcctgccgcg ttgcgtgggg cgatttgcgg
     3841 ttgagtgtgg cgggcgctca cttcgacgcg gaggacgccg cagaggcgcg gaaggcgcgg
     3901 agaagacctt gaaaaccttt ccgtcctctg cgccctctgc gcctctgcgt cctcggcgtt
     3961 aaatcctgcc ccggtacgct caaccgcaca gcgcttcaag caaagccgct gcctaccgtg
     4021 tttttacaac ccttttacct catcgcaatg attttggcgg ggcgcgtggc taccatgacg
     4081 gctcagcctg ttcgggaaaa cccgtgggcc gccggaagtt gccacgcgtc gcgccgccag
     4141 agtctcccaa gatagtgaat taatctcacc ccacgcgcga gggaacggtt ggcatatcgg
     4201 ggcggagggc tttagactcg gctcctgaac ggatgtgtcg gcggcgggcg ccgccgcggc
     4261 ccgcggcttc agcaaacgag cgacggggca tggaacgact cgtgcagatt ctgttcgggc
     4321 atgagcgggc cgttttcacc aacggccagt tcggcttcgg cctgcgcccg caccccgccg
     4381 tcctcgtcgc gggcgcgctc ctcttctgcg ccttcatcta cttcgtctac gtccgcccgc
     4441 gcgcgcggct gcgcggcccg aagctcgcgg cgctcatctc gctgcgcgtc gcgctcttcg
     4501 cgctgctcgt cttcctgctg ctgcggcccg tcgtcgtcgt cccctcggtc gtcccgcgca
     4561 gcacctcggt cgcgctcgtc gcagacgact cgcgcagcat gcagatcgcc gacgcgcccg
     4621 gacgcaggac gcggctcgaa gccgtgcgcg ccgcgctctt ctccgcggag acgaagttcc
     4681 tgccgcggct gcaagagaag ttcaggacag acctctacgc cttctcgggc gagcttggcg
     4741 cgctcggcgg cggcggcgaa gagctgttcg gcgagggcgc ggcgagcgat ctgggcggcg
     4801 ccgtctccga ggccgcgcgg cgcgcggcgg gcgtgccgct gtcggcggtc gtgctcgtgt
     4861 cggacggcgc ggcgaacgtg ccgcgcgacc tcggcgcggt cttgcgcgag ctgcgcgcga
     4921 agaacctgcc ggtctacacc gtgggggtcg ggagcgccga gcgcccgacg gacgcggaac
     4981 tggtgcgcgt gcagatgccg cggcgcgtgc tcgtcggttc gagcgtcaac gtcgaggcgt
     5041 tcgtgcgcct ctcgggctac ggcccgacga aggtcttgat cgcggtgagc gaggacgggc
     5101 gcgcggtcaa gaccgaggag ttcaacctgc gcggcggcga gaccgaggcc gtccgcctag
     5161 agctgacggc ggcgattgcg ggcgtccacc gctacacgtt cgagatcact gcgctcgacg
     5221 gcgagatgac gctggagaac aacgggcgcg aggcgctggt cgaggtcatc gaggggcctc
     5281 tgcgcgtgct ctacgtcgag ggcgagccgc gctgggagca cggcaagatg cgctccgccg
     5341 tgacgcgcaa cgagaagaac gtggagctgg tgtcgattct gcgcaccggc gagaacaagc
     5401 tctaccggca gggcatctcg ggcgagcagg agctggccgc cggcttcccc gcgaccgagg
     5461 aggagctgtt cgcttacgac ggcttgatgc tcgggagcgt cgaggcaggg ttcttcacc
//
LOCUS       DBNGOH010000436         5306 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2631998, whole genome shotgun sequence.
ACCESSION   DBNGOH010000436 DBNGOH010000000
VERSION     DBNGOH010000436.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5306)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5306)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5306
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2631998"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..631
                     /gene="thiE"
                     /locus_tag="AC159P_08635"
     CDS             <1..631
                     /gene="thiE"
                     /locus_tag="AC159P_08635"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HIR3695557.1"
                     /translation="ALPIDLPRIYPITDRRLTGLAHAEQVARLCEGGARLIQLREKHL
                     TPREFYREAADALRVARSCGARIIINDRADIALALGADGLHLGQDDLRPDAARRVLGD
                     DAIVGFSTHNAAQATEAALLPVSYVAVGPVFGTASKENPDPVVGLDTLRRVRSITEGI
                     PLVAIGGITHANARAVLEAGADSLAVIGAVLSQDPADITRRMRDFLHAL"
     gene            968..2194
                     /locus_tag="AC159P_08640"
     CDS             968..2194
                     /locus_tag="AC159P_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HIR3695558.1"
                     /translation="MRYFDLPPLIERMLQVSDKISDLNFSVGQAPQVEINGRLTAVQP
                     QGLQKLTPYQTELIAMTLLQGNPDAAHRLAHIGSADLSYSLPSRTRFRVNVFQQRGTY
                     SIVMRVIPTEVPTLDTLHLPKQLADITELRNGVVLVTGPTGAGKSSTLAALIDLINEK
                     HHYHVVTIEDPIEFLHTHKSSTINQREIGSDAKDFPSALRAALRQAPKVILIGEMRDY
                     ETTEIALEAAETGHLVLSTLHTIDASKTVDRIIGLYPKNEEQVIRTRLAQVFRFIVSQ
                     RLIPRADGQGRTAAIEILKSTPRTREYISEGEGEGKTLLDAMRDGRHDGMQDFDTVIR
                     GMIERQTVTLEDGLSFATNPNNLQLALKGMSAAEDFLVPEKTAGAAPRHHPRPGHTPV
                     SPLRQGSGASMLDMIE"
     gene            2263..3030
                     /locus_tag="AC159P_08645"
     CDS             2263..3030
                     /locus_tag="AC159P_08645"
                     /inference="COORDINATES: protein motif:HMM:TIGR02098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HIR3695559.1"
                     /translation="MIVTCHNCATRLQLDDAKVPSRPFTVRCPKCAQTVNAQPTAGER
                     QGSALAVVGDLPASTRAQQQMTTPAPATVFEAALSGAQAGGDVLQMLTELLRRGGEER
                     PDPRGVMRRPSWERRRALVCVGSVHGAEVSRALAARDYEVFVADKTQQAFELLHEERL
                     DVVVLDAEFDMGGQGALHLNRELNSMRMAERRRLVFVQLSPTLRTGDVQAAFLNNANL
                     IVNTGDASELAHILERNIRDLNELYRDFNRALNVAEL"
     gene            3350..4327
                     /locus_tag="AC159P_08650"
     CDS             3350..4327
                     /locus_tag="AC159P_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002633521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HIR3695560.1"
                     /translation="MASSNGHRHGHGHAGHDHHHGAGRATRSRRRLRTVLLLTAAYML
                     AEAVGGWLTGSLALLADAGHMLTDVAALALALMAVWFGSRPATPSKTFGYYRLEILAA
                     FVNGVALVLVSLFIFYEAYERWAAPPPIARGGLMMAVAAGGLLVNLACAWLLHGDHEE
                     DLNVRGAWLHVLGDALGSVGAIVAGALVTFYGWYAADPLFSALIAVLIIWSSWKLIRE
                     ATNVLLEGTPAHINLAAVEDAILATTGVEDVHDLHVWTITSGREALSAHVIHAHAVSQ
                     PELLKELRAKLLDKFGVDHLTIQMETVEFEDEAIHFCTSGTACVRSVRE"
     gene            4354..4827
                     /locus_tag="AC159P_08655"
     CDS             4354..4827
                     /locus_tag="AC159P_08655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695561.1"
                     /translation="MKQLTRYGYFLGVALLTFGAGVAAGSLSRTAVVVRPFSAAEVQV
                     RFKKTSRDYHITLEYEVLNRSDHALRYNGYSEGSNENWSVRRGEQLQSFRPFCGTGLK
                     ERTLGPGESETFQAFVEEAGPARVGFEFKVGEERVKQTFWSDEIFVAEPLSADGN"
     gene            4817..5059
                     /locus_tag="AC159P_08660"
     CDS             4817..5059
                     /locus_tag="AC159P_08660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695562.1"
                     /translation="MEINLLLVLDLREQAALQAALVTHGAPDHLVTLALTGACRIPTL
                     DEARRLRRWLADARAAGKTDFASLNVIERELIRFGV"
BASE COUNT          932 a         1857 c         1636 g          881 t
ORIGIN      
        1 cgctttgcca atcgaccttc cccgaatcta cccgatcacc gaccggcgac tgaccggcct
       61 cgcgcacgcc gagcaggtcg cgcgcctgtg cgagggcggc gcgcgcctga ttcaactgcg
      121 cgagaaacat ctcacgccgc gcgagttcta ccgcgaggcg gcggacgccc tgcgcgtcgc
      181 gcgctcatgc ggcgcgcgca tcatcatcaa cgaccgcgcg gacatcgcgc tcgccctcgg
      241 ggccgacggc ctgcacctcg ggcaggacga ccttcggcca gacgccgcgc gccgcgtgtt
      301 gggcgacgac gctatcgtcg gcttctccac gcacaacgcc gcgcaggcga ccgaagccgc
      361 gctcctcccc gtcagctacg tcgccgtcgg ccccgtcttc gggaccgcct cgaaagagaa
      421 ccccgacccg gtcgtcggcc tcgacacgct ccgccgcgtg cgctcgataa cggaaggcat
      481 cccgctcgtc gccatcggcg gcatcacgca cgccaacgcg cgcgccgtgc tcgaagcagg
      541 cgccgacagc ctcgccgtca tcggcgccgt cctctcccaa gaccccgccg acatcacgcg
      601 acgcatgcgc gacttcctcc acgcactctg agtcggcact aaaaaattaa ccacgaagac
      661 acgaagacac aaagaaagat aagaaggaga gcgacaaaga ttctcgcctt gattgttcct
      721 tatttaagga cgtttcagag tcctattttt ttgtcttcgt gtcttcgtgg ctaatctctt
      781 tcgccgcctt aagtgttaaa atacttcgcg ttaaaagttt aaacttgcct tttttgaatg
      841 aatccgccac aatggttttg gtcttcgggc gccgcgcggc gtccgctccc ggcgagcgcg
      901 cctttcgtcc cgaacgccct ccttacgtac cttactttct tcattcagaa cgagcgaacc
      961 aactggcatg agatacttcg acctgccgcc tctcatcgag cgcatgctcc aagtctctga
     1021 caagatcagc gacctcaact tctccgtcgg gcaggcgccg caggtggaga tcaacggcag
     1081 gctgaccgcc gtccagccgc aaggcttgca gaagctgacg ccctaccaga ccgagctgat
     1141 cgcgatgacg ctcttgcagg gcaaccccga cgccgcgcac cgtctcgcgc acatcggctc
     1201 ggcagacctc agctactctc tgccctcgcg cacgcgcttc cgcgtcaacg tcttccagca
     1261 gcgcggcacc tactccatcg tgatgcgcgt catcccgacc gaggtgccga cgctcgacac
     1321 gctccacctg ccgaagcagc tcgccgacat cacggagctg cgcaacggcg tcgtcctcgt
     1381 caccggcccg accggcgcgg gcaaaagctc gacgctcgcc gccctcatcg acctcatcaa
     1441 cgagaagcac cactaccacg tcgtcaccat cgaagacccc atcgagttcc tccacacgca
     1501 caaaagctcg accatcaacc agcgcgagat cggatcggac gcgaaggact tcccctcggc
     1561 cctgcgcgcc gcgctcaggc aggcgccgaa ggtcatcctc atcggcgaga tgcgcgacta
     1621 cgagacgacc gagatcgcgc tcgaagccgc cgagaccggc cacctcgtcc tctcgacgct
     1681 gcacaccatc gacgcgtcga agaccgttga ccgcatcatc ggcctctacc cgaagaacga
     1741 agagcaggtc atccgcacgc ggctcgcgca agtcttccgc ttcatcgtct cgcagcgcct
     1801 catcccgcgc gccgacgggc agggccgcac cgccgccatc gaaatcctca agtccacgcc
     1861 gcgcacgcgc gagtacatca gcgagggcga gggcgagggc aagacgctgc tcgacgcgat
     1921 gcgcgacggc aggcacgacg gcatgcagga cttcgacacc gtcatccgcg ggatgatcga
     1981 gcgccagacc gtcacgctcg aagacggact ctcgttcgcc accaacccca acaacctcca
     2041 gctcgcgctc aaaggcatgt ccgccgccga agacttcctc gtccccgaga agaccgccgg
     2101 cgccgcgccg cgccaccatc cgcgccccgg ccacacgccc gtctcccccc tgcgccaagg
     2161 ctccggcgcc tcgatgctcg acatgatcga ataagtctga agtctgaagt cggaaaaatg
     2221 cttttgctca gacttcagac tccagactcc agacttgaat ccatgatcgt cacttgccac
     2281 aactgcgcca cccgccttca gctcgacgac gcgaaggtgc cttcgcgccc gttcaccgtg
     2341 cgctgcccga agtgcgcgca gaccgtcaac gcgcagccga cggcgggcga gcggcagggg
     2401 agcgcgctcg ccgtcgtcgg cgacctgccg gcctcgaccc gcgcgcagca gcagatgacg
     2461 acgcccgcgc ccgcgacagt cttcgaggcg gcactctcgg gggcgcaggc cggcggcgac
     2521 gtgttgcaga tgctgacgga gcttctgcgg cgcggcggcg aggagcgacc cgacccgcgc
     2581 ggcgtgatgc gcaggccgtc gtgggagcgg cgccgcgcgc tcgtctgcgt cggctcggtg
     2641 cacggcgctg aggtgtcgcg cgcgctggcg gcgcgcgatt acgaggtctt cgtcgccgac
     2701 aagacgcagc aagcgttcga gctgctgcac gaggagcgcc tggacgtggt ggtgctcgac
     2761 gcggagttcg acatgggcgg gcagggcgcg ttgcacctca accgcgagct gaactcgatg
     2821 cggatggccg agcgccgccg cctcgtcttc gtccagctct ccccgacctt gcgcaccggc
     2881 gacgtgcagg ccgccttcct caacaacgcc aacctcatcg tcaacaccgg cgacgcctcc
     2941 gaactcgccc acatcctcga aaggaacatc cgcgacctca acgaactcta ccgcgacttc
     3001 aaccgcgcgc tgaacgtggc cgagctgtag agagcagcgg gcagcaggca gcgggcagcg
     3061 ggcagtaaag gcgagaggct tgggaggtaa agtcccaagc ctcttacacc ggctgcttga
     3121 tgaagcaaga ggcttgggac tttacctccc aagattcttc actcttcatt cttaactgcc
     3181 tgctgcctgc tgcccgctgc cgactgcttt ttgctttccg ccgtaatctg ataatcttag
     3241 tcgccgccgg gcggtaactc tcttcctttc caccgtggga ttaagctgtc gcggcgcgcg
     3301 gctccggcgc gggcgcctgc ggaatcttat cgttgacggt gaggcgcgga tggcttcgtc
     3361 gaacggacac cggcacggac acggtcacgc ggggcacgac caccaccacg gggcgggtcg
     3421 cgcgacgcgc agccgccgaa gactgcgcac cgtgctcctg ctgaccgccg cctacatgct
     3481 ggccgaggcg gtgggcggtt ggctgaccgg gagcctcgcg ctgctggccg acgccggcca
     3541 catgctgacg gacgtggcgg cgctggcgct cgcgctgatg gccgtctggt tcggctcgcg
     3601 gcccgcgacg ccgagcaaga cgttcggcta ctaccggctg gaaatcctcg cggccttcgt
     3661 caacggcgtg gcgctcgtcc tcgtctcgct cttcatcttc tacgaagcct acgagcgatg
     3721 ggcggcgccg ccgcccatcg cgcgcggcgg cctgatgatg gcggtcgcgg cgggcggcct
     3781 cctggtcaac ctcgcgtgcg cgtggctgct gcacggcgac cacgaggagg acttgaacgt
     3841 gcgcggcgcg tggctgcacg tgttgggcga cgcgctcggc tcggtcggcg ccatcgtcgc
     3901 gggcgcgctc gtcaccttct acggctggta cgccgccgac ccgctcttca gcgccctcat
     3961 cgcggtgctc atcatctgga gttcgtggaa gctcatccgc gaggcgacga acgtgctcct
     4021 cgaaggcacg cccgcgcaca tcaacctcgc cgcggtcgag gacgcgattc tggccacgac
     4081 cggcgtcgag gacgtgcacg acctgcacgt ctggacgatc acctcggggc gcgaggccct
     4141 ctcggcgcac gtcatccacg cgcacgccgt ctcgcagccg gagctgctga aggagctgcg
     4201 cgcgaagctc cttgataagt tcggcgtcga tcacctgacg atccagatgg agaccgtgga
     4261 gttcgaggac gaggccatcc acttctgcac ctcgggcacc gcctgcgtcc gctccgtgag
     4321 ggagtaggcg cagcccgccc cttgaaggtt cgtatgaaac agctaacacg ttacggctac
     4381 tttctcggcg tcgcgctcct gacgttcggc gcgggcgtcg cggcggggtc gctctctcgt
     4441 accgccgtcg tcgtgcgccc cttctcagcc gcggaggtgc aagtcaggtt caagaagact
     4501 tcgcgcgatt accacatcac gctggagtac gaggtgctga accggagcga ccacgctctg
     4561 cgctataacg ggtactccga gggcagcaac gagaattgga gcgtcaggcg cggcgagcaa
     4621 ttacaaagct ttcgaccttt ctgcggaacc gggctgaagg agcgcaccct cggccccggc
     4681 gagtccgaga cgtttcaggc gttcgttgag gaggccgggc cggcgcgggt gggtttcgag
     4741 ttcaaggtcg gcgaagagcg tgtgaagcaa acattttgga gcgatgagat attcgtcgcc
     4801 gaaccgttga gcgccgatgg aaattaatct cctgctcgtc ctcgatctgc gcgaacaggc
     4861 ggcgctgcaa gcggcgctcg tgacgcacgg cgcgcccgac catctggtga cgctggcgct
     4921 cacgggcgcg tgccgcatcc cgacgctcga cgaggcgcgc aggctccgcc gctggctcgc
     4981 agacgcgcgc gccgccggca agaccgactt cgcctcactc aacgtcatcg agcgcgagct
     5041 gatacggttc ggagtgtgat aaagagtagg cggtaggcgg taggcagtag gcagtaaaca
     5101 tcggattcac acttcaattc taaacttcga agttccagct ttcttgactg cctattgcct
     5161 accgcctact gcctgctaat acttatggag atcgaactcg gatacgggcg ggggacggtg
     5221 cccttcgatt tggaggaggg gcggttcgag gtgctcgaag gcgaggcggg cgcgggggcc
     5281 gtgttgtcgg acgcggagat cggggc
//
LOCUS       DBNGOH010000437         2378 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4648504, whole genome shotgun sequence.
ACCESSION   DBNGOH010000437 DBNGOH010000000
VERSION     DBNGOH010000437.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2378)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2378)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2378
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4648504"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="AC159P_08665"
     CDS             complement(<1..255)
                     /locus_tag="AC159P_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015126745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695563.1"
                     /translation="MRAATPEDERERLEALRHFKVLDTDPEEAFDDITRLASYICQTP
                     SALISFVDADRQWFKARVGFEQQESSRDVSFCAHAILQPGP"
     gene            652..1740
                     /locus_tag="AC159P_08670"
     CDS             652..1740
                     /locus_tag="AC159P_08670"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HIR3695564.1"
                     /translation="MFEGEGAVGLLKRVRGGAVGLLLVSVAVASACASAHGQEGAGAE
                     TLFSESRPAMGTTFEVYLYAADRARADALFEAAFEEIERVEAVFSNYRPASELSRINR
                     QAASAPVVTDPEVFGLLERAFAFGRMSGGAFDMTVGKLMKAWGFFRGAGRYPSEAELK
                     RALDQIGWRRVRLDAAARTVRFAAPGIELDPGGIGKGYALDRVAAVLREAGVKAALLT
                     SGSSSVYALGAPPGKKGWPVRVPDPLDRARVLSTVVLRDQSLSTSGNYEKFFRLGGRT
                     YCHIMNPRTGHPVEGMLQTTVIAPEATDSDALSTAVFVLGPQRAAALLARVKGTAALL
                     VTDKTGAGRVVEINWPRTRATASGASGR"
     gene            1824..>2378
                     /locus_tag="AC159P_08675"
     CDS             1824..>2378
                     /locus_tag="AC159P_08675"
                     /inference="COORDINATES: protein motif:HMM:NF013567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR3695565.1"
                     /translation="MNRRKFVGVVSAGVASAGTALLGEGARAQQAKGAARAVAPSDEI
                     GVGVIGPGSRGREIMRHFMRVPGVKVRAVADVYEPRFAETRRLTKEETPAYTDYRKML
                     ERKDLDAVIVASPLNLHAEHVVASVESGRHVYGEKSLGYSVEHCNRIVAAVRRSGKHF
                     QVGHQYRYASWFHEAVRRINAGEIG"
BASE COUNT          374 a          735 c          875 g          394 t
ORIGIN      
        1 cgggccgggt tgcagaatgg cgtgcgcgca gaaggaaacg tcgcgcgaag actcctgctg
       61 ctcgaagccg acgcgcgcct tgaaccactg gcggtcggcg tccacgaacg agatgagcgc
      121 cgagggcgtc tggcagatgt aagaggcgag gcgcgtgatg tcgtcgaacg cctcctccgg
      181 gtcggtgtcg agcaccttga agtggcggag ggcttcgagc ctctcgcgct cgtcctccgg
      241 tgtcgctgct ctcatcgctc gcgctccgct gcgtgggtct ttaaagccgt gttgctttta
      301 tgatggaagc cacgcgggaa agcaagcgcg cgcgcctgag tttgatttcg aacaggcgac
      361 gttaacacga ctggaggggc acggcttgtc cctgcccgct aacgttcgtt agattcaacg
      421 ttgagtgaac acgtgagcgc gggccgcgga caagccctgc ccctacagat tcagaggcgc
      481 tgtgagttcc agcgacggtt gagtgagccg ggaaagaata gaccgcggat tcggtttgag
      541 gtatgttacc ggtatcggca cgggaaatgt agtattatcc tgtcgcacgg cgcgattaag
      601 acttaaaaga tgtgggcggc ggcttcatga gaagcgaggt tgtctttgag tgtgttcgaa
      661 ggcgaggggg cggtcggttt gttgaagcgt gtgcgcgggg gcgcggtcgg gttgctcttg
      721 gtgtcggtgg cggttgcgtc ggcgtgcgcg tctgctcacg ggcaggaggg cgcgggcgcc
      781 gagacgctgt tctccgagtc gcgcccggcg atgggcacca cgttcgaggt ctacctgtac
      841 gccgcagacc gcgcgcgcgc cgacgcgctg ttcgaggcgg cctttgaaga gatcgagcgg
      901 gtcgaggccg tcttcagcaa ctaccgtccg gcgagcgagc tgtcgcgcat caacaggcag
      961 gcggccagcg cgccggtcgt gaccgacccc gaagtcttcg gcctgcttga gcgcgcgttc
     1021 gctttcggcc ggatgagcgg cggcgcgttc gacatgaccg tggggaagct gatgaaggcg
     1081 tggggcttct tccgcggcgc gggccgctac ccttcggagg cggagctgaa gcgcgcgctt
     1141 gaccagatcg gttggcggcg cgtgcggctc gacgcggcgg cgcgcacggt gcgcttcgcc
     1201 gcgcccggca tcgaactcga ccccggcggc atcggcaagg gctacgcgct cgaccgcgtc
     1261 gccgcagtgc tgcgcgaggc gggcgtcaag gcggcgctgc tcacttcggg ttcgagcagc
     1321 gtctacgcgc tgggcgctcc gccgggcaag aagggctggc ccgtgcgcgt acccgaccct
     1381 ttggatcgcg cgcgcgtgct ctcgaccgtc gtgctcaggg accagtcgct ctcgacttcg
     1441 ggcaactacg aaaagttctt ccggctcggc gggcgcacct actgccacat catgaacccg
     1501 cgcacgggcc accccgtcga ggggatgttg cagaccaccg tcattgcccc ggaggcgacc
     1561 gacagcgacg cgctctcgac cgccgtcttc gtgctcggcc cgcagcgcgc cgcggccctg
     1621 ctcgcgcgcg tgaagggcac ggcggcgctg ctcgtcacgg acaagaccgg cgccgggcgc
     1681 gtggtcgaga tcaactggcc gcggacgagg gcgaccgcgt ccggcgcgtc cgggcgttga
     1741 cttcggcagt caacgccgac gggtaaaagt gttatcttga attccgttca cgcggagatg
     1801 tcgcagggag agggaagtca cagatgaaca gaagaaaatt cgtgggcgta gtttcggcgg
     1861 gcgtggcctc ggcgggcacg gcgttgctgg gcgagggcgc tcgcgcgcag caggcgaagg
     1921 gcgcggcgcg cgcggtggcg ccgtcggacg agatcggcgt cggcgtcatc gggccgggca
     1981 gccggggccg cgagatcatg cgccacttca tgcgcgtgcc gggcgtgaaa gtgcgcgccg
     2041 tggccgacgt gtacgagccg cgcttcgcgg agacgcgccg gctgacgaag gaagagacgc
     2101 cggcctacac cgactaccgc aagatgctcg agcggaaaga cctcgacgcc gtcatcgtcg
     2161 cctcgccgct gaacctccac gccgagcacg tcgtcgcgtc ggtcgagtcg gggcggcacg
     2221 tctacggcga gaagtctctg ggctactcgg tcgagcactg caaccgcatc gtcgcggcgg
     2281 tgcgccgcag cggcaaacac tttcaggtcg gccaccagta ccgctacgcg tcgtggttcc
     2341 acgaggccgt ccggcgcatc aacgcgggcg agatcggc
//
LOCUS       DBNGOH010000438         7206 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3917075, whole genome shotgun sequence.
ACCESSION   DBNGOH010000438 DBNGOH010000000
VERSION     DBNGOH010000438.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7206)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7206)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7206
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3917075"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            261..878
                     /locus_tag="AC159P_08680"
     CDS             261..878
                     /locus_tag="AC159P_08680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695566.1"
                     /translation="MKRRTLLGVSRVLWCAALLSLAVPAAHAKAKGAARQREGSQIDA
                     TVVSIGGRASGRSRPFTLRIDRFTSTDEVQRLNSALQSGGQDELLKVLSDMDAGRIAV
                     GNNVGVDANAIIAAPREGGGTRLIVLFQRTINFYELRAGARSTDYRFGYAEIILDRDG
                     KGEGTFIPVARVRLHDGNQWEVEDFGTFPARIMGARVRGGRGRAR"
     gene            complement(999..2975)
                     /locus_tag="AC159P_08685"
     CDS             complement(999..2975)
                     /locus_tag="AC159P_08685"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012154873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 97 protein"
                     /protein_id="HIR3695567.1"
                     /translation="MTRLTKGLLLMLAPLVCLAPDAAAQTGYSVRSPDGRIEVRVRTG
                     ERVRYDVLLKGRALLQDSTMSLDVDGKTLGLAPKVLAAKQTARDAVVEPPVRQKAARI
                     HERYRELRLEMEGGYAVVFRAYDEGAAYRLETSLARAEVKVYGEEVALNFADNYTVYY
                     PQEESLFSHNERLYLPRPLKEIAPAAFATLPAVVDAGGVKVAVAESDVEDYPGLWLRG
                     TNANGLAAMFPPYPLKEELAASGGKTSDRDFKVTEAADYIALTKGTRTFPWRLLGVAE
                     RDGDLITNPLVYLLAPPSRIQDTSWIKPGKVAWDWWNANNVHGVDFKSGINTETYKFY
                     IDFAAQHGIEYVVLDEGWYKLGNVLEVVPEINVEELVAYGKQKNVGIILWVIWKTLDD
                     QLEPALDRYAKWGVKGVKIDFMQRDDQKVMNFYHKVSREAAERRLLVDYHGGQRAVLL
                     ARTWPNIVSNEGVRGLEWSKWSADAEPEHNVTLPFTRMFLGPMDYTPGAMVNASQKSF
                     AKIFETPMSLGTRAHQLAMYVVYESPLQMLADSPSNYLREPEAMQFLSAVPTVWDETR
                     VLDGKLGDYVLVARRSGREWYVGAMTDWTRRELEVDFSFLPAGAFRMEAYQDGANADR
                     FGSDYKKVSSRVSKSDKLKITLAPGGGWAARLRP"
     gene            3170..4588
                     /locus_tag="AC159P_08690"
     CDS             3170..4588
                     /locus_tag="AC159P_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020400642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HIR3695568.1"
                     /translation="MRFSAPLLAVLCVATLAGCTQQQQGGTVSTTNTNRTTGANPYQP
                     TPYVRLRHPEWSKNAVIYQINTRQFTEEGTFRAAETHLPRLKQLGVDILWLMPIHKIG
                     EKNRKGTLGSPYSVKDYYSVNPEFGTLEDLKHFVAAAHAQGMYVILDWVANHTAWDNN
                     LAAEHPEWYERDYKGDFRPTPWWDWSDIIDLDYKHEGLRRYMTEALKYWVREADIDGY
                     RCDVAGFVPVDFWNNARKELDAIKPVFMLAEWESRDLHAEAFDMTYAWSWYDAVHQIT
                     QGKKTDLSSLFIYYSWNESSYPRESMRMTFVSNHDKNAWEGTEFEAFGKGLEAAMVLS
                     AIGEGMPLVYNGQEAGNTRRLQFFEKDPIVWKEHPNGALYQKLFALRKKNTALWNAQW
                     GATMILVPNSAPAKVFSFVRHNDTDKVFAVLNFSDQPQSVTFKETLYHGQYTDCFGGQ
                     PVALDAATRLELKPWAYHVFVK"
     gene            complement(4807..5535)
                     /locus_tag="AC159P_08695"
     CDS             complement(4807..5535)
                     /locus_tag="AC159P_08695"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015152306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HIR3695569.1"
                     /translation="MKILMLFLTAAMLTVALPGDPEGFDMIDAASQAQENVKVRNVAV
                     FVHEGVELLDFAGPGEVFAAAHTKGGAHAFNVYTVAASAEPIISQGFLSVRPQYTFEN
                     APRPDIVVLPGGRTNIPLQNPKVVEWVKKTSRDAEVMMSVCTGAFLLARAGLLDGKEA
                     TTHWGSIERLKQEATKTTVLENRRFIDNGKVVTTAGVSAGIDGALHVVARLLGQEEAR
                     RTARYMEYLWTPEKNADKQVSSER"
     gene            complement(5821..6696)
                     /locus_tag="AC159P_08700"
     CDS             complement(5821..6696)
                     /locus_tag="AC159P_08700"
                     /inference="COORDINATES: protein motif:HMM:NF014376.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR3695570.1"
                     /translation="MNERSKEIVADAHLKPGEFFGDVLRKQRCAGLVLSEVRHRTGRR
                     LPVHSHDLAYFCLLLGGNYSERVGRRTLTYKPLTVVFHPPGLTHRDEVGGDGGHFFSV
                     EMEARWFESLREYTAAPAAVTEARGDDLAWLATRLYREFREPDAASPLAVEGLVMAML
                     TDVARAAASVGEREPRWLARAVEMLHDEGGERLTVGSVAAAVGVHPFHLSRVFKRFRR
                     MTVGEYLNALRIERACRELERPASSIIDVALSAGFSDQSHFTRAFKRATGMTPGAFRR
                     ARGADAGSAPLPEEE"
     gene            complement(6745..>7206)
                     /locus_tag="AC159P_08705"
     CDS             complement(6745..>7206)
                     /locus_tag="AC159P_08705"
                     /inference="COORDINATES: protein motif:HMM:NF051698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HIR3695571.1"
                     /translation="DDRGLSDNDQRHRLVVSGALEAPTGSDDGLRRLLRGFRLGYIFT
                     YASHLPFNILTGNDRNFDTNTNDRPAGLGRNTGRGFDFASLDLRLSRRLRLGERVALE
                     VLAEGFNVLNRANLAVPNNTFGAGASALPAFGQPTAAFDPRQLQFGLRLDF"
BASE COUNT         1269 a         2422 c         2287 g         1228 t
ORIGIN      
        1 cggcgcgaaa ccgtgctcaa ctggaagacg cggaattccg ccgccgaaag gatcaggcgg
       61 gcgcgcgccg tcatgaaatt tttcttcgaa cgcgcgccga cgcgcccccc ttcgcactgg
      121 cgggatggat tcggcaacaa ctcttggtat cattaccgcg tacagctacg agtgcgagca
      181 gtcgagaggg cgcggggttt ctttaaagtc actccgcccg tcatctactc cgaacgcgct
      241 gtttcgaaga tggagggaac atgaagagaa ggacactact cggcgtgtcg cgcgtgttgt
      301 ggtgcgcggc gttgctgtcg ctcgcggtgc cggcggccca cgcgaaagcg aagggcgcgg
      361 cccgccagcg cgaggggtcg cagatcgacg ccacggtcgt cagcatcggc gggcgcgcct
      421 cgggcaggtc gcgcccgttc accctcagga tcgaccgctt cacgtccacg gacgaggtgc
      481 agcggctgaa ctccgcgctc cagtcgggcg ggcaggacga gttgctgaag gtgctgtcgg
      541 acatggacgc cgggcggatc gccgtcggca acaacgtcgg cgtggacgcc aacgccatca
      601 tcgccgcgcc gcgcgaggga gggggcacgc ggctgatcgt cctcttccag cggacgatca
      661 acttctacga gctgcgcgcg ggcgcgcgct cgaccgacta ccggttcggc tacgcggaga
      721 tcatcctcga ccgcgacggc aagggcgagg ggacgttcat ccccgtcgcg cgcgtccgtc
      781 tgcacgacgg caaccagtgg gaggtcgaag acttcggaac cttccccgcg cgcatcatgg
      841 gcgcgcgcgt ccgcggcggc aggggccgcg cgcgctgagc cgtcatcgcg ggaataggaa
      901 ttaaccacaa agacacgaag acacagctaa agaagaagtg gcttttgtct ttagctatgt
      961 cttcgtgtct ttgtggttaa tcgtcttctt aactgaaact agggccgcag cctcgccgcc
     1021 catccgccgc ccggcgcaag tgtgattttg agtttgtctg acttgctcac ccgactcgac
     1081 accttcttgt agtcgctgcc gaagcggtcg gcgttcgcgc cgtcctgata ggcctccatg
     1141 cggaaggcgc ccgcgggcag gaaggagaag tccacctcca gctcgcgccg cgtccagtcg
     1201 gtcatcgcgc cgacgtacca ctcgcgcccg ctcctgcgcg cgaccaagac gtagtcgccg
     1261 agcttgccgt cgagcacgcg cgtctcgtcc cagaccgtgg ggacggcgga gaggaactgc
     1321 atcgcctccg gctcgcgcag gtagttcgag gggctgtcgg cgagcatctg caaggggctt
     1381 tcgtagacga cgtacatcgc cagttggtgc gcgcgcgtgc cgaggctcat cggggtctcg
     1441 aagattttgg cgaagctctt ctgcgaggcg ttgaccatcg cgcccggcgt gtagtccatc
     1501 gggccgagga acatccgcgt gaacggcagc gtgacgttgt gctccggctc tgcgtccgcg
     1561 ctccacttgc tccattcgag gccgcgcaca ccctcgttgc tgacgatgtt cggccacgtc
     1621 cgcgcgagca gcaccgcgcg ctgcccgccg tgataatcga ccagcagcct gcgctcggcg
     1681 gcctcgcgcg agaccttgtg atagaagttc atcaccttct ggtcgtcgcg ttgcatgaag
     1741 tcgatcttga cgcccttgac gccccacttc gcgtaccggt cgagcgccgg ttcgagctgg
     1801 tcgtcgagcg tcttccagat gacccacagg atgatgccga cgttcttctg tttgccgtag
     1861 gccaccagct cttcgacgtt gatttccggc accacctcaa gcacgttgcc gagcttgtac
     1921 cagccctcgt ccagcacgac gtactcgatg ccgtgctgcg cggcgaagtc gatgtagaac
     1981 ttgtaggtct cggtgttgat gcccgacttg aaatcgacgc cgtggacgtt gttcgcgttc
     2041 caccagtccc aagccacctt gcccggcttg atccaagagg tgtcctgtat gcgcgacggc
     2101 ggggcgagca ggtagacgag cgggttcgtg atgaggtcgc cgtcccgctc ggcaacgccc
     2161 agcagccgcc acgggaacgt gcgcgtcccc ttcgtcaagg cgatgtagtc cgccgcctcc
     2221 gtcaccttga agtcgcggtc ggacgttttg cccccgctcg ccgccagctc ctctttcaaa
     2281 gggtaaggcg ggaacatggc cgcgaggccg ttcgcgttcg tgccgcgcag ccacagcccc
     2341 ggatagtcct ccacgtccga ctcggcgacg gccaccttga cgccgcccgc gtccaccacc
     2401 gcgggcagcg tggcgaaggc tgccggggcg atctccttca gcggtcgcgg caggtacagc
     2461 cgctcgttgt gcgagaagag gctctcctcc tgaggatagt agacggtgta gttgtccgcg
     2521 aagttgagcg cgacctcctc gccgtagacc ttcacctccg cccgcgcgag agaggtttcg
     2581 aggcggtagg ccgcgccctc gtcgtaggcg cggaagacga ccgcgtagcc gccctccatc
     2641 tccagccgca gctccctgta gcgctcgtgg atgcgcgccg ccttctgccg caccggaggc
     2701 tcgacaacag cgtcgcgggc ggtctgcttc gcggccaaga ccttcggcgc gaggcccaac
     2761 gtcttcccgt ccacgtcgag cgacatcgtc gagtcctgaa gcagcgcccg gcccttcagc
     2821 agcacgtcgt aacggacgcg ctcgcccgtg cggactcgca cctcgattct cccgtcgggc
     2881 gagcgcacgc tgtagcccgt ctgcgccgcc gcgtccgggg ccaagcagac caacggcgcc
     2941 aacatcaaca gcaacccctt cgtcaatcgt gtcatctctg atctactctt ccggtttcgt
     3001 ctgcacgagc gccgtctcga atatcacatc tcgcggcgcc gtacaacgaa ccgccgcgcg
     3061 cacgaagcgc attgacacgg cgtccgggaa gctgatactt caagtcatcg cctcaggcac
     3121 gctcgaacgt cttagtcgct ttcattgcac ccgaagggag atcagtcgaa tgcgcttctc
     3181 ggcaccgctg ttggccgtcc tctgcgtcgc gacactcgcg ggctgtactc aacaacaaca
     3241 aggtggcacg gtgagcacga ccaacacaaa ccgaacgacc ggcgcgaacc cctaccagcc
     3301 gaccccctac gtcaggctca ggcaccccga gtggtcgaag aacgccgtca tctaccagat
     3361 caacacgcgg cagttcacgg aggaaggcac cttccgcgcg gcggagacgc acctgccgcg
     3421 gctgaaacag ctcggcgtgg acatcctgtg gctgatgccg attcacaaga tcggcgagaa
     3481 gaaccgcaag ggcacgctcg gcagccccta ctcggtcaag gactactaca gcgtcaaccc
     3541 cgagttcggc acgctcgaag acctcaagca cttcgtcgcg gcggcccacg cgcaaggcat
     3601 gtacgtcatc ctcgactggg tggccaacca cacggcttgg gacaacaacc tcgccgccga
     3661 acaccccgag tggtacgagc gcgattacaa gggcgacttc cggcccacgc cgtggtggga
     3721 ctggtcggac atcatcgacc tcgactacaa gcacgaaggc ttgcgccgct acatgacgga
     3781 ggcgctcaaa tactgggtac gcgaggcgga catcgacggc taccgctgcg acgtggcggg
     3841 cttcgttccc gtcgatttct ggaacaacgc gcgcaaggag ctggacgcca tcaagcccgt
     3901 cttcatgctc gccgagtggg agtcgcgcga cctccacgca gaggcgttcg acatgaccta
     3961 cgcgtggagc tggtacgacg ccgttcatca aatcacgcaa ggcaagaaaa cagacctcag
     4021 ctccctcttc atctactact cgtggaacga aagctcgtac ccgcgcgaga gcatgcgcat
     4081 gaccttcgtc agcaaccacg acaagaacgc ttgggagggc acggagttcg aggcgttcgg
     4141 caaaggtctg gaagcagcga tggtgctctc ggccatcggc gaagggatgc cgctcgtcta
     4201 caacgggcag gaggcgggca acacccggcg gctccagttc ttcgagaaag accccatcgt
     4261 ctggaaagag cacccgaacg gcgcgctcta tcaaaagctg ttcgccctcc ggaagaagaa
     4321 caccgcgctc tggaacgcgc agtggggcgc gaccatgatc ctcgtgccca acagcgcccc
     4381 cgccaaagtt ttcagcttcg tccgccacaa cgacacggac aaggtcttcg ccgtcctcaa
     4441 cttctccgac cagccgcagt ccgtcacctt caaagaaacc ctctaccacg gccaatacac
     4501 ggactgcttc ggcggccaac ccgtcgcact cgacgccgcg acccgcctcg aactgaagcc
     4561 gtgggcttac cacgtcttcg tcaagtaaga ggagtctccg gcccgcgcct cgcatcgtcg
     4621 cgctcggggc cgtgacggtc gccgaaagac agtaggcagt aggcagtaag cagttaagac
     4681 agaagaacga agaggcttgg gaggtaaagc ccaaccctcc tcgctcttca agcgataaag
     4741 gaagaggctt gagacgttgc atctcaagcc tcttgctctt gctgcctacc gcctaccgtt
     4801 tcctgctcac cgctcactgc tcacttgctt gtctgcgttc ttctcgggcg tccagaggta
     4861 ttccatgtag cgggctgtgc gccgggcctc ttcctggccg aggaggcggg cgacgacgtg
     4921 gagcgcgccg tcgatgccgg cggagacccc ggcggtcgtg accaccttgc cgttgtcgat
     4981 gaaacggcgg ttctcgagca cggtcgtttt ggtcgcctcc tgtttgagtc tttcgatcga
     5041 cccccagtgc gtcgtcgcct ccttgccgtc gagcagcccg gcgcgcgcga gcaggaacgc
     5101 gcccgtgcag accgacatca tcacctctgc atcgcgcgac gttttcttca cccactcgac
     5161 gaccttcggg ttctgcaaag ggatgttggt gcgcccgccc ggcaggacga cgatgtcggg
     5221 ccgcggggcg ttctcgaacg tgtactgggg gcgcaccgag aggaagccct gactgatgat
     5281 cggctcggcg gaggcggcga ccgtgtagac gttgaaggcg tgcgcgccgc ccttcgtgtg
     5341 ggcggcggcg aagacctctc cggggccggc gaagtcgagc aactcgaccc cctcgtggac
     5401 gaagacggcg acgtttctca ctttcacgtt ctcctgcgcc tgcgaggccg cgtcgatcat
     5461 gtcgaagccc tcggggtctc cgggcaacgc gaccgtcagc atcgcggcgg tcaaaaacag
     5521 catcaagatt ttcataaccc actcctttcg agaccatgcg aatccgtcac gcccccatca
     5581 tcgcgcgcgg cccggccccg cgtcttgcac gatacaaacc cgatgggacg aaacaaacat
     5641 tcgcacgcgc cgactgacat tcatctcttc agagtgtttt gcaagaccac tcggcgggcg
     5701 tcacgactgt aggggcaagg cttgtccgcg gcccgtcacg tctcgatgga ttcagcgttc
     5761 cagtgattgc gtgggcacgg gcagggacaa gcccggcccc tacagtcttg ccacgcgctc
     5821 ttactcttct tccggcagag gcgcgcttcc ggcgtccgcg ccgcgcgcgc ggcggaaggc
     5881 gcccggcgtc atgccggtcg cgcgtttgaa ggcgcgcgtg aagtggctct ggtcggagaa
     5941 gcccgcggag agggccacgt cgatgatgct cgacgcgggg cgctcaagct cgcggcaggc
     6001 gcgctcgatg cggagcgcgt tcaggtactc gccgacggtc atgcggcgga agcgcttgaa
     6061 gaccctcgac aggtgaaagg ggtggacgcc gaccgccgcg gcgacgctgc cgacggtgag
     6121 gcgctcgccg ccctcgtcgt gcagcatctc gacggcgcgc gcgagccagc gcggctcgcg
     6181 ctccccgacg ctggcggcgg cgcgcgccac gtccgtcagc atcgccatca ccagcccctc
     6241 gacggcgagc ggcgacgcgg cgtccggttc gcggaactcg cggtagagcc gcgtcgccag
     6301 ccacgcgagg tcgtcgccgc gcgcctcggt gacggcggcg ggcgccgccg tgtactcgcg
     6361 cagcgactcg aaccagcgcg cctccatctc gacgctgaag aagtggccgc cgtcgccgcc
     6421 cacctcgtcg cggtgcgtga ggcccggcgg gtggaagacg accgtcagcg gcttgtaggt
     6481 cagcgtccgg cggcccacgc gctccgaata gttcccaccg agcagcaggc agaagtacgc
     6541 cagatcgtgc gagtgcacgg gcaagcgccg ccccgtccgg tgccggactt cggagagcac
     6601 gaggcccgcg cagcgctgct tcctcagcac gtcgccgaag aactcgccgg gcttcaagtg
     6661 cgcgtccgcg actatctctt tacttctttc gttcatgaca tcacttccgt tactcgtgac
     6721 gaccaccgcc ttccttcgcg cgactcagaa gtcgaggcgc aggccgaact gtagctgccg
     6781 cgggtcgaag gcggcagtcg gttggccgaa cgcggggagc gcgctcgcgc ccgcgccgaa
     6841 ggtgttgttc gggacggcga ggttggcgcg gttgagcacg ttgaagcctt cggccagcac
     6901 ctccagcgcg acgcgctcgc cgaggcgaag acgacgactg aggcgcaggt cgagcgaggc
     6961 gaagtcgaaa ccgcggcccg tgttgcgacc cagaccagcg gggcggtcgt tcgtgttggt
     7021 gtcgaagttg cggtcgttgc ccgtcaggat gttgaagggc agatgcgagg cgtaggtgaa
     7081 gatgtagccc aaccgaaagc cgcgcagcag acgacgcaga ccgtcgtcac ttcccgtcgg
     7141 cgcttcgagc gcgccgctga cgactaagcg atgacgctgg tcgttgtctg aaaggccgcg
     7201 gtcgtc
//
LOCUS       DBNGOH010000439         4247 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4282169, whole genome shotgun sequence.
ACCESSION   DBNGOH010000439 DBNGOH010000000
VERSION     DBNGOH010000439.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4247)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4247)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4247
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4282169"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(124..1128)
                     /locus_tag="AC159P_08710"
     CDS             complement(124..1128)
                     /locus_tag="AC159P_08710"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013134.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HIR3695572.1"
                     /translation="MRLPKFTYHVPRTIGEAVRMMGEAGPSGQFVAGGTDLYPNMKRR
                     QQTPRTVISVGRLKELQRIEGDGKSGVIIGAGVSLTDIVEHPVINRDYPAVARAARTI
                     STPVLRNMGTVGGNLLLDTRCNYYDQNYEWRRGINFCLKKDGDVCWVAPGSSKCWAVQ
                     SADLVPLMVAMGARVRLVSTVGDRMVTAKGLYNDDGIEYLHKRPEELLTEIHLPPVNG
                     SRAVYKKLRRRGAFDFPVLGVAASLDFAEDGTVREAKVVLGGIAPAPLEVEAAGAALA
                     GGPLDEERMRAAAEACYLRARPLDNTDHLMNYRKQMARPFVLRALQELKAGRGERETG
                     "
     gene            complement(1156..1923)
                     /locus_tag="AC159P_08715"
     CDS             complement(1156..1923)
                     /locus_tag="AC159P_08715"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF042649.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HIR3695573.1"
                     /translation="MPIENLSFADRRVFDVENPETGVAFAEAVVLAESKFGTIGTVGS
                     YTPPRSPGRFKGAGVGPSPAYSYSAAVAEVDVDAETGLVTVERIHIGHDIGKSINPML
                     VMGQVEGSVYMGLGEALMEEMVYRANRNVVHKFPSLLEYKSPTTMEMCDVVTYLVEDA
                     DPNGPFGAKEVGQGPLLPVMPAVANAVYDAVGVRIDEVPITPEKVLAALRLKAQGKDP
                     RFGPNELPPVVWPDPLKVLTPDEGGDGKEMPRVAVHS"
     gene            complement(1913..3592)
                     /locus_tag="AC159P_08720"
     CDS             complement(1913..3592)
                     /locus_tag="AC159P_08720"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014758.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HIR3695574.1"
                     /translation="MKTNGKETNGADLKVVGKPFRKVDARSKCVGETRFADDIALPRM
                     LYCKILRSPEPHARIVSIDTSKALALPGVHAVITGKDLPITYGILPVSQDEHPLCVER
                     VRFIGDPVAAVAAVDEDTAFDAMNLIEVVYEKLQSITSIEEAVLIEEPRIHDYGDGGN
                     VHKKVHLEFGDMEAGFAEADLVREDIFFYEGNTHLPMEQHAALAHFDSDDKLTLWSST
                     QTPHYVHRALAKTLELPAAHIRVIATPNGGGFGGKSDPFNHEIVVAKLSMLTRRPVKV
                     TLTREEVFYCHRGRHPVLMRSKIGVRKDGSITALDFESLLDGGAYGSYGVASTFYTGA
                     LQTVTYEVPRYRFRGLRAFTNKPPCGPKRGHGTPQPRFGLEIQLDKIADQLDLDPAEM
                     RLKHLAKPYSVTANYLRLGSMGLGPCIEKVVKGSDWKNKHRKLPYGKGVGIACSSYIC
                     GAGLPIYWNNMPHSGVQLRLDRQGGVCVQCGSTDIGQGSDSVLAYVVAEILGIDPFDI
                     RVVTADTDLTPVDLGSYSSRVTLMTGNAAIQAAERARAADHRRLGKARRAD"
     gene            complement(3599..4129)
                     /locus_tag="AC159P_08725"
     CDS             complement(3599..4129)
                     /locus_tag="AC159P_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015860623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HIR3695575.1"
                     /translation="MTETTRQKAHIEFTLNGEPTEVAFAPHKTLLEVLREDLALTGTK
                     HGCELGECGTCAVLVDGRSILSCLMLGLDAEGREVETVEGLATAEGLHPLQTTFADTG
                     AAQCGYCTPGFLLVAKELLKTNPRPSREEIKEALSGNLCRCTGYIKIYEAVELAAARL
                     RGEEAELPRESVYGYE"
BASE COUNT          671 a         1347 c         1444 g          785 t
ORIGIN      
        1 aactaccgca agcagagggc gcgccctttc gtgcggcgcg cgctgcaaga actgacggca
       61 ggacggggag aaagggagac ggggtgatgg ggcgaggggg agagaagaat tcgaatcgcc
      121 ccgtcacccc gtctcccttt ctccccgtcc tgccttcagt tcttgcagcg cgcgcagcac
      181 gaaagggcgc gccatctgct tgcggtagtt catcaggtga tccgtgttgt cgagcgggcg
      241 cgcgcgcagg taacaagcct ccgccgccgc gcgcatacgc tcctcgtcga gcgggccacc
      301 ggcgagcgcc gcgcccgcgg cctcgacttc gagcggcgcg ggcgcgatgc cgccgaggac
      361 gaccttcgcc tcgcgcaccg tcccgtcttc cgcgaagtcg agcgaggccg cgacgccgag
      421 caccgggaag tcgaacgccc cgcgccgccg cagcttcttg tagaccgcgc gcgacccgtt
      481 cacaggcggc aggtgtatct ccgtcagcaa ctcctccggc ctcttgtgca ggtactcgat
      541 gccgtcgtcg ttgtacagcc ccttcgccgt caccatccta tcgcccacgg tcgagacgag
      601 ccgcacccgc gcgcccatcg ccaccatcag cggcaccagg tctgctgact gcaccgccca
      661 acacttcgag ctgcccggcg cgacccagca cacgtcgccg tccttcttca gacagaagtt
      721 gatgccccgc cgccactcgt aattctggtc gtaataatta caccgcgtgt cgagcagcaa
      781 attgccgccc acggtgccca tgttcctgag caccggcgtc gagatcgtgc gcgccgcacg
      841 cgccaccgca gggtagtcac ggttgataac cgggtgctcc acgatgtcgg tcaggctgac
      901 gcccgcgccg ataatcacgc ccgacttgcc gtcgccctcg atgcgttgca gctctttcaa
      961 acgcccgacc gagatcaccg tccgcggcgt ctgctgccgc cgcttcatgt tcgggtaaag
     1021 gtcggtcccg cccgccacga actgccccga aggccccgcc tcgcccatca tcctcaccgc
     1081 ctcgccgatg gttcggggca cgtggtacgt gaatttcgga agtctcatca taagggtcga
     1141 ttcaatccca ctgttctatg aatgcacggc cacgcggggc atctctttgc cgtcgccgcc
     1201 ttcgtcgggg gtgagcacct tgagcgggtc gggccagacg acggggggca gttcgttggg
     1261 gccgaagcgc gggtctttgc cttgtgcctt caaacgaagg gcggcgagga ctttttcggg
     1321 ggtgatgggc acttcgtcaa tgcggacgcc gacggcatca taaaccgcgt tggcgacggc
     1381 gggcatgacg gggaggagcg ggccttgccc gacttccttg gcgccgaagg ggccgttggg
     1441 gtctgcgtct tcgacgaggt acgtcaccac gtcgcacatc tccatcgtcg tcgggctttt
     1501 gtattcgagg agcgagggga atttgtggac gacgttgcgg ttggcgcggt agaccatctc
     1561 ctccatcagc gcttcgccca accccatata gacggagcct tcgacctgcc ccatgacgag
     1621 catggggttg atggacttgc cgatgtcgtg gccgatgtgg atgcgctcga cggtgacgag
     1681 gccggtctcc gcgtccacgt ccacctcggc gaccgccgcc gagtagctgt aggcgggcga
     1741 ggggccgacg cccgcgccct tgaaacgtcc gggcgagcgc ggcggcgtgt aggagccgac
     1801 cgtgccgatg gtgccgaact tactttcggc gaggacgacc gcctcggcga aggcgacgcc
     1861 cgtctccggg ttctccacgt cgaagacgcg gcggtcggcg aaagacaaat tctcaatcgg
     1921 cacgccgagc ttttccgaga cggcgatggt cagcagctcg cgcgcgctcg gcggcttgaa
     1981 tcgcggcgtt gccggtcatc agcgtgacgc ggctcgaata gctgccgaga tcgaccggcg
     2041 tcaagtcggt gtcggcggtg acgacgcgga tgtcgaaggg gtcgatgccg aggatttcgg
     2101 cgacgacgta ggcgagcacc gagtccgacc cttgcccgat gtcggtcgag ccgcattgga
     2161 cgcagacgcc gccctgtcta tcaagacgaa gctgcacgcc cgagtgcggc atgttgttcc
     2221 agtagatggg cagccccgcg ccgcagatgt aagagctgca cgcgatgccc acgcctttgc
     2281 cgtaagggag cttccggtgc ttgttcttcc agtcggagcc tttgacgact ttttcgatgc
     2341 aggggccgag gcccatcgag ccgaggcgga gatagttggc cgtcaccgag taaggcttcg
     2401 ccagatgctt caggcgcatc tccgccgggt cgaggtcgag ctggtcggcg atcttgtcga
     2461 gctggatttc gaggccgaag cgcggctgcg gcgtgccatg cccgcgcttg gggccgcagg
     2521 gcggcttgtt ggtgaatgcg cgcaggccgc gaaaacggta gcgcggcact tcgtacgtga
     2581 cagtttgcag cgcgccggtg tagaaggtcg aggcgacgcc gtaggagccg taggcgccgc
     2641 cgtcgagcag cgactcgaag tcgagcgcgg tgatcgagcc gtccttcctg acgccgattt
     2701 tgcttctcat caaaacgggg tgccgaccgc ggtggcagta gaagacctcc tcgcgcgtca
     2761 aggtgacctt gacgggccgg cgcgtcagca tcgagagctt ggcgacgacg atctcgtggt
     2821 tgaaggggtc gctcttgccg ccgaagccac cgccgttggg cgtggcgatg acgcggatgt
     2881 gcgcggcggg cagctccagc gtcttggcga gcgcgcggtg gacgtagtgc ggcgtctgcg
     2941 tcgaagacca gagcgtgagc ttgtcgtccg agtcgaagtg cgcgagggcc gcgtgctgct
     3001 ccatcggcag gtgcgtgttg ccctcgtaga agaagatgtc ttcgcggact aaatcagcct
     3061 cggcaaagcc cgcttccatg tcgccgaatt cgaggtggac tttcttgtgg acgttgccgc
     3121 cgtcgccgta atcatgaatg cgcggctctt cgatgagcac ggcctcttcg atgctcgtga
     3181 tgctttgcag cttctcgtag acgacctcga tgaggttcat cgcgtcgaag gccgtgtcct
     3241 cgtccacggc ggcgaccgcc gcgacggggt cgccgatgaa gcgcacgcgc tcgacgcaca
     3301 gagggtgctc gtcctgactg acgggcagga tgccgtaagt gatgggcaag tccttgcccg
     3361 tgatgacggc atggacgccg gggagggcga gcgccttcga cgtgtcgatg ctcacaatgc
     3421 gcgcgtgcgg ctccggcgag cggaggattt tgcagtagag catgcgcggc agcgcgatgt
     3481 cgtcggcgaa gcgcgtctcg cccacgcact tcgagcgcgc atccaccttg cggaagggct
     3541 tgccgacgac cttcaagtcc gcgccgttcg tttctttccc gttcgtcttc atcgaattct
     3601 attcgtagcc gtagacgctc tcgcgcggca gctcggcctc ttcgccgcgc aggcgcgcgg
     3661 cggccagctc gaccgcctcg taaatcttga tgtagccggt gcagcggcag aggttgccgg
     3721 agagggcttc cttgatctct tcgcgcgaag gccgcgggtt cgtcttcaac agctctttgg
     3781 cgacgaggag gaaaccgggc gtgcagtagc cgcactgcgc ggcgccggtg tcggcgaagg
     3841 tcgtttgcag cgggtggaga ccttcggcgg tcgccagccc ctcgacggtc tcgacctcgc
     3901 gcccctcggc gtcgaggccg agcatgaggc atgaaagtat cgagcgaccg tcaacgagga
     3961 cggcgcacgt gccgcactcg cccagctcgc agccgtgctt cgtgccggtc agtgcgaggt
     4021 cttcgcgcag gacttcgagc agcgtcttgt gcggcgcgaa ggcgacctcc gtcggctcgc
     4081 cgttgagggt gaactcgatg tgcgctttct gtcttgtggt ctcggtcatc ggttggattt
     4141 cgaattgagt tcggcctgct tgcgcacgga cggcccggcg ggcgtgtgcg cagaaggggc
     4201 atgataacag ttttgtagtc ggtgcttaag cggggccgcg gggcggg
//
LOCUS       DBNGOH010000440         4336 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2450643, whole genome shotgun sequence.
ACCESSION   DBNGOH010000440 DBNGOH010000000
VERSION     DBNGOH010000440.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4336)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4336)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4336
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2450643"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="AC159P_08730"
     CDS             <1..201
                     /locus_tag="AC159P_08730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695576.1"
                     /translation="RRVRTLFSGSAWRRRETAPAYHFDAGAFDLPGPPTGLQYTREAY
                     LQDFLRSAAPPKKTLTSGAGPV"
     gene            801..2696
                     /locus_tag="AC159P_08735"
     CDS             801..2696
                     /locus_tag="AC159P_08735"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019351.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase family protein"
                     /protein_id="HIR3695577.1"
                     /translation="MPKISKSRRKEAKEAKKNRLEIIKAQLTRREMIRMGLLTSAGLL
                     IPKRGLSARARDSAGHFIDDIPCDSPPTANFPLGWDLPTAMNGLMPVIQPFTSASDLT
                     GPAPTVAPNTAINPATGLPFEGRTRPHQFATRFNPVKFYEVHQRRFLGNVHPDYPLQP
                     LWGFGRKTDLANVAFSPGVTYVAHYGEPVMVRNFNDLPDDNEGFGLNQVTTHLHNGHT
                     ASESDGFPCDWYRRGQYYDQRYANVLAGFDSTHPPNGDIRESMSTLWYHDHRVEFTAQ
                     NVYKGLAGFYLLFNEFDTGVEGQGFNLPAHPNFDIPLIIADRSFDEDSELLCFDLGNF
                     DGILGDKFLVNGRITPTFAVQPRRYRFRVLDGGPARFYQLFLTNLSSLGSNNQFWQIS
                     NDGNLLPRPVRVSSFRLGVAERADIIIDFSEYKGKTIYLENRLRQDDGRGPMGEQVVG
                     AGQGNRLLKFVVSNTAVADPSADPATQVFYQLPDTSKTDPTIVPRVRRTFEFTRSGGD
                     GWRINGRLMPGDCSENRFTVQRNSVEEWTFVNSSGGWHHPIHIHMEEFQILNRSRGGA
                     VPNATVENARKDVVRLRDNETVKVFFRWRDWRNFYPIHCHNTIHEDHAMMLTWSIEDV
                     GDMIQRP"
     gene            2727..3410
                     /locus_tag="AC159P_08740"
     CDS             2727..3410
                     /locus_tag="AC159P_08740"
                     /inference="COORDINATES: protein motif:HMM:NF014667.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HIR3695578.1"
                     /translation="MPNINRRNWLAASGAALVTGGLTTTARAGAPCGEGPRAAVSARE
                     RIQRRHFPNLPLVTHEGREVRFYDDLVKDKIVLLNMMYAKCDGICMPTTMNLVKVQKL
                     LREHYGARFGREIVMYSITLKPEQDSAEVLRKYAITHGAGPGWLFLTGKPDDIEQLRR
                     AQGYVDLDPEIDKARISHTGNVRYGNEARTIWSACPGLSKPAAIAEAISWVDWPKSGP
                     GTAAKGEVK"
     gene            3407..>4336
                     /locus_tag="AC159P_08745"
     CDS             3407..>4336
                     /locus_tag="AC159P_08745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HIR3695579.1"
                     /translation="MKATLDRARVLLAALALAVFAAAPAAAQQTTTEVPLDTEPTVGT
                     DPAPAPGPEQVPTGDGGEAPAPTTTAVSLGGAEAIAFDGTYVWVARQFRNSVTRVRAG
                     DNVNGGTFKVGDNPVAVLYDGTSVWVANLESDTVTKLRPSDGAVLGTYAAGDGPSGLA
                     HGGGVLWVTNRNTNKVTKLRTSDGVLLGAYAVGSRPIGVAVLGTSVFVANNLADTVTK
                     LRASDGALLGTYAVGDGPLGLASDGTNLWVTTFFSDSLMKVRATDGVKLGTYSVGKAP
                     AGIAFSGTYIWAANSGNNTVIQVRPADGAVLSSY"
BASE COUNT          808 a         1572 c         1339 g          617 t
ORIGIN      
        1 cggagggtaa gaacgctctt ctccgggagc gcttggcggc ggcgcgaaac cgcgcccgcc
       61 taccacttcg acgcgggcgc gttcgacctg cccggcccgc ccaccggctt gcagtacacg
      121 cgcgaggctt acctccaaga cttcctccgg agcgccgcgc cgccgaaaaa aacattgact
      181 tcgggagcgg ggccggtata gattccctcc gctcaacaga cagtttccac gtagcttgcc
      241 caactgtcct cattgacggg gctcaaaact ctcgaaggcc gggcctcgcg accggcaaga
      301 gaagaggagt tctgtctatc acactgactg ctacaagcgt acagggtgcg ttcgtccttg
      361 cggcgcgttt ggccggcaag ctcgaacgat cgaataaact ctcgatgccg cccgttcttg
      421 ctcggcgcaa gcgtctctgc ctttaagaga gtcttccgcc gccggtcgtt tccccgtgcg
      481 accgccgcgc cctccgtggc cgaagtccgg tgccgcgtcc cctaacttca gcaggttcct
      541 tctcaacaat acggccttca cgcgacgccc cctcagcgcg gcggcgaccg caccaccgcg
      601 aagccccgcg agaggcgcgc gcgacaaagg caggcttcaa cctcaaaaca cgggctacct
      661 gtgacaactc aacctacgga aggaacgcgg cccggcgcct cgcctcgcgg aagccgccgc
      721 gcttccggta ctcatcaacc ccacgggacg accgcgtgcg tctcgccgcg cgcggcggaa
      781 gcgacaggag aaaaacccca atgccgaaaa tttcgaaatc ccgacggaag gaagcgaagg
      841 aagcaaaaaa gaacagactg gagataatca aggcccagtt gacgcggcgc gagatgatcc
      901 ggatgggtct tttgacgagc gccgggctgc tcattcccaa gcggggcctc agcgcccgtg
      961 cgcgcgacag cgcgggccac ttcatcgacg acatcccgtg cgacagcccg ccgaccgcca
     1021 acttcccgct cggctgggac ctgcccaccg cgatgaacgg cctgatgccc gtcatccagc
     1081 ccttcacctc tgcgagcgac ctgaccggcc cggcgccgac cgtggccccg aatacggcca
     1141 tcaaccccgc gaccggtctg cccttcgagg gccgcacgcg cccgcaccag ttcgccaccc
     1201 gcttcaaccc ggtcaagttc tacgaagttc accagcgccg cttcctcggc aacgtccacc
     1261 cggactaccc gttgcagccg ctctggggct tcgggcgcaa gaccgacctg gccaacgtcg
     1321 ccttcagccc cggcgtcacc tacgtcgcgc actacggcga acccgtcatg gtgcgcaact
     1381 tcaacgacct gccggacgac aacgagggct tcggcctcaa ccaggtcacg acgcacctgc
     1441 acaacggcca caccgcctcg gagtcggacg gcttcccgtg cgactggtac aggcgcggcc
     1501 agtactacga ccagcgctac gcgaacgtgc tcgccggctt cgactcgacc cacccgccca
     1561 acggcgacat ccgcgagtcg atgtccacgc tctggtatca cgaccaccgc gtcgagttca
     1621 ccgcgcagaa cgtctacaag gggctggccg gcttctacct gctcttcaac gagttcgaca
     1681 ccggcgtcga ggggcagggc ttcaaccttc cggcgcaccc caacttcgac atcccgctga
     1741 tcatcgccga ccgcagcttc gacgaggact cggagctgct ctgcttcgac ctcggcaact
     1801 tcgacggcat cctcggcgac aagttcctcg tcaacggcag gatcacgccg accttcgccg
     1861 tgcaaccgcg ccgctaccgc ttccgcgtgc tcgacggcgg cccggcccgc ttctatcaac
     1921 tgttcctgac caacctctcg tcgctcggct ccaacaacca gttctggcaa atctcgaacg
     1981 acggaaacct gctgccgcgc cccgtgcgcg tctccagctt ccgtctgggc gtcgccgagc
     2041 gcgccgacat catcatcgac ttcagcgagt acaagggcaa gaccatctat ctcgaaaacc
     2101 gtctccggca ggacgacggg cgcggcccga tgggcgagca ggtggtcggc gcggggcagg
     2161 gcaaccggct cctcaagttc gtcgtcagca acacggcggt cgccgacccg agcgccgacc
     2221 cggcgacgca agtcttctac cagctgcccg acacgtcgaa gaccgacccg accatcgtcc
     2281 cgcgcgtgcg ccgcaccttc gagttcacgc gcagcggcgg cgacgggtgg cgcatcaacg
     2341 ggcggctgat gcccggcgat tgcagcgaga accgcttcac cgtccagcgc aacagcgtcg
     2401 aggagtggac gttcgtcaac agctcgggcg gctggcacca cccgatccac atccacatgg
     2461 aggagttcca aatcctcaac cgcagccgtg gcggcgcggt gccgaacgca acggtcgaga
     2521 acgcgcgcaa ggacgtggtg cggctgcgcg acaacgagac cgtcaaggtc ttcttccgct
     2581 ggcgcgactg gcgcaacttc taccccatcc actgccacaa cacgatccac gaggatcacg
     2641 ccatgatgct cacgtggagc atcgaggacg tgggcgacat gatccagagg ccgtaagccg
     2701 tcacaccagg aaaggagcta cgagttatgc cgaacattaa ccgaagaaac tggctcgcgg
     2761 cgtccggggc ggccctcgtg accggcggcc tcacgacgac ggcgcgggcg ggcgcgcctt
     2821 gcggtgaagg gccgcgggcc gcggtctcgg cgcgcgagcg gattcagcgg cgccacttcc
     2881 ccaacctccc gctcgtcacg cacgagggca gggaagtccg cttctacgac gacctcgtca
     2941 aagacaagat cgtcctgctc aacatgatgt acgccaagtg cgacggcatc tgcatgccga
     3001 cgacgatgaa cctcgtcaag gtgcagaagc tcctgcgcga gcactacggc gcgcggttcg
     3061 ggcgcgagat cgtgatgtac tcgatcacgc tcaagcccga gcaggacagc gccgaggtgc
     3121 tcaggaagta cgccatcacg cacggcgccg ggccgggctg gctctttttg acgggcaagc
     3181 ccgacgacat cgagcagttg cggcgcgcgc aaggctacgt tgacctcgac cccgagatcg
     3241 acaaggccag gatcagccac accggcaacg tgcgctacgg caacgaggcg cgcacgatct
     3301 ggtcggcgtg ccccggcctg agcaagccgg cggccatcgc cgaggcgatc tcgtgggtgg
     3361 actggccgaa gagcgggccg ggcacggcag cgaaaggaga ggtgaaatga aagcgactct
     3421 ggacagagca agagttctct tggccgccct cgcgctggcg gtcttcgcgg cggcgcccgc
     3481 cgccgcacaa cagacgacga ccgaagtccc gctcgacacc gagccgaccg tcggcaccga
     3541 ccccgccccc gcccccggcc ccgaacaggt gccgaccggg gatggcggcg aggcgcccgc
     3601 ccccaccacc accgcggtca gcttgggcgg cgccgaggct atcgccttcg acggcaccta
     3661 cgtctgggtg gcgcggcagt tcaggaactc ggtgacgcgc gtgcgcgcgg gcgacaacgt
     3721 caatggcggc accttcaagg taggcgataa cccggtcgcg gtgctctacg acgggacgag
     3781 cgtctgggtg gccaaccttg agagcgacac ggtgacgaag ctcaggccgt cggacggcgc
     3841 cgtgctcggc acctacgccg cgggcgacgg cccgagcggg ctggcccacg gcggcggcgt
     3901 cctctgggtg acgaaccgca acacgaacaa ggtgacgaag ctgcgcacgt cggacggcgt
     3961 gctgctcggc gcctacgccg tcggcagccg ccccatcggc gtcgccgtcc tcggcacgag
     4021 cgtcttcgtg gcgaacaacc tcgcggacac cgtgacgaag ctgcgcgcct cggacggcgc
     4081 cctgctcggc acctacgccg tcggcgacgg ccccctcggg ctggcctcgg acggcaccaa
     4141 cctgtgggtg acgaccttct tcagcgacag cctgatgaag gtgcgcgcca ccgacggcgt
     4201 caagctcggc acctattcgg tcggcaaggc gcccgccggc atcgccttca gcgggactta
     4261 catctgggcg gcgaacagcg gcaacaacac cgtcatccag gtgcggcccg ccgacggcgc
     4321 cgtgctcagc tcctac
//
LOCUS       DBNGOH010000441         9494 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1168419, whole genome shotgun sequence.
ACCESSION   DBNGOH010000441 DBNGOH010000000
VERSION     DBNGOH010000441.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 9494)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9494)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9494
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1168419"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            245..1720
                     /locus_tag="AC159P_08750"
     CDS             245..1720
                     /locus_tag="AC159P_08750"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HIR3695580.1"
                     /translation="MKLSEAASLMGASALGANSEVFDKELKGFALDSRAVAPGELFFA
                     LSPEDLRRHCFTDTSAADAHRFIPGALGRGAAAAVARGARVEGDRELQAVRGPLLLVE
                     DVIEALQMLARGVLEKWGGPVVGITGSAGKTTTKDLTAHVLGASGRRVLRSRKNFNNE
                     LGVALSVLQMESEGARPQEFDVAVLEMGMSLPGEIARHCTVAPPDVAVVLTVAPVHLE
                     FMGSVEAIAEGKAQIVEGLKKGGTAVLNADDALVAAMRARHAGRVITYGIDHPADVSA
                     TDIERAGLGLTRFRLRTPLGEAKVELPMPGRHNLSNALAAAAVATSFDLAPGTIAEAL
                     STAAPSQMRGEVLSFAEGFTVVDDSYNSNPRSLLAMAEAVAQGGDEVKRRVIVAGEML
                     ELGAESAALHREAGRDIAGLGVDVVWGVRGDARELVEGAREAGLSEGATAFFETSEEA
                     AQALAGFVRAGDLVLVKGSRGVHMERVVEALRAKCRMQSAE"
     gene            1783..2937
                     /gene="mraY"
                     /locus_tag="AC159P_08755"
     CDS             1783..2937
                     /gene="mraY"
                     /locus_tag="AC159P_08755"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020712185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HIR3695581.1"
                     /translation="MIYYIFYELLYRNLAGGSESYFVKALNVFQYVTFRTAYSTITAL
                     LISLVFGGRVIRALRNLNVGQQIREEGPQAHMAKRGTPTMGGVLVIGSVLISTLLWAR
                     LSSEYVWIILIATTLFAAIGFLDDYAKVAKKQSLGLKGRQKLVGQFLIAAFVWGSLYL
                     LTEYGYANYTWRLSLPFFKDTALTRITEIGPWLYLPFIVIVLTGFSNAVNLTDGLDGL
                     AISVTVVAMATLTAFTYVSGDARWAAYVGLEHQKDIGELTVFCGAMVGASLGFLWFNA
                     PPAEVFMGDVGSLAIGGAIGTIGVLTKQEFILILIGGVYVLEALSVTLQVGFFKLTKR
                     TQGTGRRLFKMTPLHHHFEMLDWKEPKIVFRFLIIAILFALLSLSTLKLR"
     gene            3034..4437
                     /gene="murD"
                     /locus_tag="AC159P_08760"
     CDS             3034..4437
                     /gene="murD"
                     /locus_tag="AC159P_08760"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HIR3695582.1"
                     /translation="MDVNGKKVLVIGAARSGVAASRFLAARGAVVALNDRKPFVDWPN
                     EALRLKGEGVGLLAGEVPSWLLDQVELVVLSPGVPTKSITARYADRAGAEVIGEVELA
                     WRFLRGRVVGITGTNGKTTTTALVGRLLEEAGLPVQVGGNIGTPLISLVESSREDGWT
                     VAELSSYQLETIKEFRPTVALVLNLMPDHMDRYDSLADYGAAKHRIFRNQTAADVAVL
                     NADDEIVASWARGLNAHVVMFSTERELEEGLFLRGGRELVARTLAGERVLMTREELQL
                     KGVHNVQNVLAALAAGLACGASPESMRATVRGFGPVEHRLERVAEVGGVTYYNDSKAT
                     NVDAAVKAVEALADEPGEIVLILGGRGKNAPYAPLAPLVRGKGRKLVVLGEDADRIED
                     ELKAFAPVARAKDMAEAVRLAHAAARPGDIVLLAPACASFDMFTGFEHRGRVFKEEVN
                     RLRTADCGLRNEKANAL"
     gene            4482..5792
                     /gene="ftsW"
                     /locus_tag="AC159P_08765"
     CDS             4482..5792
                     /gene="ftsW"
                     /locus_tag="AC159P_08765"
                     /inference="COORDINATES: protein motif:HMM:TIGR02614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HIR3695583.1"
                     /translation="MARKLQPDEWLFAVTVALALFGVIMVYSASAVVAAVGEAHGQYH
                     YVARQGVWTLIGLGAMLFGARLDYGWLRSEVVVYGLLVLTVVLLVAVFAFPPINGARR
                     WIRFAGFSAQPSELAKLTLAIFLARLLERRAGDEGNFWRTFVPCVAVAGLLVLLVVAE
                     PDLGTAMMLGVIAVVILFTAGVRLKHMALAAAPALIGLAGLLLFVSWRLKRLAVFLDP
                     WADPQGAGYQIIQSLLAVGSGGVNGLGFTEGRQKLFFLPFAHSDFIFAVVSEELGLLG
                     GLGVVALFGLFLWRGMRAALNAPDRFGMLLATGIVAGIVAQALFNMSVVLALVPTKGI
                     PLPFISYGGSSLVPTLFAVGVLLNISQHAGLGDKGAWRGWGAETRGRGDAGAQSRTKG
                     AAASPKRPVSTGATAAAQPSRPHASAPHAATPRRAFAAALRRAG"
     gene            5798..6892
                     /gene="murG"
                     /locus_tag="AC159P_08770"
     CDS             5798..6892
                     /gene="murG"
                     /locus_tag="AC159P_08770"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HIR3695584.1"
                     /translation="MRVLIAAGGTGGHIYPGIAVAKEVMRRDPSSVVRFVGTARGLET
                     RLVPQAGFELSLIESTGLVNMGWRERVRGLAVLPKSLAAARRLIRSFRPDIVVGAGGY
                     VSGPVLLTAALMRLPTLVMESNAVPGFTNRRLALFVRAAAVSFEAALPYFRGKGVVTG
                     NPIRREFFEVPAKERDPSRFSLLIFGGSQGARAVNEAMVAALPHLAEHRAVLRVTHQT
                     GKLDFEKVREGYRAAGWGETADVREYIDDMVAAFAAADLVVSRAGATTSFELMAAGRA
                     AVMVPLPGQLEQRRNAEAMQDAGAARMILQEELSGERLAREIAALVKDPEQLTRMGRA
                     SRKLARGDAAAATVDLMEKLVGSRQQAVGS"
     gene            7040..8434
                     /gene="murC"
                     /locus_tag="AC159P_08775"
     CDS             7040..8434
                     /gene="murC"
                     /locus_tag="AC159P_08775"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HIR3695585.1"
                     /translation="MFRRIQHVHLVGIGGIGMSGIGEVLLNMGFRVSGSDLRRSEVTN
                     RLEKLGATIFEGHAAENMTGAPHVVVRSTAVRDDNPEVVEARRRSIPVIARAEMLAEL
                     MRLKPHTVAVAGSHGKTTTTSMIATVLAHANLDPTVVVGGVVGAMGSNARVGKSDLMV
                     VEADESDRSFLMLTPTFAVVTNIDREHMDYYADMMDVRKCFTDFVNKVPFYGASVLCL
                     DDPHVQAVIPEVVRRRITYGLSAQADISAREVRYNQQFGSTFQVRQFGETLGEVTLNV
                     PGLHNVYNALAATAIGLELEVEFDVIAGALADFRGVNRRFQFKGEEAGVIVVDDYGHH
                     PTEIRATLVAAKLGTTGRRMVVLFQPHRYTRTQDLMDEFARSFNNADVLMVTDIYAAS
                     EDPIEGVNAEALVEAVKRYGHKDARHVGSVEDATRALLEEVREGDMVITLGAGNVYRV
                     SEELPGLLKERAQG"
     gene            8512..>9494
                     /gene="murB"
                     /locus_tag="AC159P_08780"
     CDS             8512..>9494
                     /gene="murB"
                     /locus_tag="AC159P_08780"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HIR3695586.1"
                     /translation="MEEIAARLGVRADRGRRFAELTSLRVGGAIDWVLLPETEEQAAG
                     VVGALEAAGVAWRVLGAGSNVLADDREHRYVVVSMREVKGEARFAGERVSVSAGFSLP
                     RLCVEAARQGLSGIEGLGGIPGTVGGALWMNAGAYGHEIGEVVESVRVACGGAVVEVP
                     GREVKWNYRHTSFKEGELLLGCTLKLKPDDPAEIKARMEEAKQKRFATQPHGARSAGC
                     FFKNPPGSGLSTGKMIDEMGMKGERRGGAVVSPVHANFIVTEGEGASAADALALAEEI
                     RERFRREHNVELEYEVELWGADREFRDEKGRAEDEGAAAVSRARDEGAGGV"
BASE COUNT         1502 a         2827 c         3630 g         1535 t
ORIGIN      
        1 gaggatgttc ggcgcagggt ggcgggctgc cgcgaccgtt tgcgccgtaa ccgcgcggcg
       61 cgcagaatta cggaacgcgc cgcgccgagc gccgccgaaa gtccggtcga agcgttggaa
      121 gcggagaccg cttcggaggg gcgcgaggcg cgagaggaga agggttgaga ggcgaccggc
      181 cttccttgag aaagtggcgc gcaggccgac gtaagaatct tgcgcgtaaa ggatggggcc
      241 gggcgtgaaa ctgagcgaag cggcaagttt gatgggcgcc tccgcgctgg gggcgaactc
      301 ggaggtcttt gacaaagaac taaaaggatt cgcactcgac tcgcgcgccg tcgcgccggg
      361 cgaactgttc ttcgccctct cgccggagga cttgcggcgt cactgcttca cggacacttc
      421 ggcggcggac gcgcatcgct tcatccccgg tgccctcggg cgcggggcgg cggcggcggt
      481 cgcgcgcggt gcgcgggtcg agggcgacag agagcttcag gcagtgcgcg ggcctctgct
      541 gctggtcgag gacgtgatcg aggcgttgca gatgctggcg cggggcgtgc tcgaaaaatg
      601 gggcggcccc gtcgtcggca tcacgggcag cgcgggcaag acgacgacga aggacttgac
      661 ggcgcacgtg ctcggcgcga gcggtcggag ggtcttgcgg agccgcaaga acttcaacaa
      721 cgagctgggc gtcgcgctct ccgtcttgca gatggagtcg gaaggggcga ggccgcagga
      781 gttcgacgtg gccgtcctcg aaatggggat gtcgctgccc ggcgagatcg cgcggcactg
      841 cacggtcgcg ccgcccgacg tggccgtggt attgacggtc gcgcccgtgc atctcgaatt
      901 catgggttcg gtcgaggcga ttgccgaagg caaggcgcag atcgtcgaag gcttgaagaa
      961 ggggggcacg gcagtgctga acgcggacga cgcgctcgtc gccgcgatgc gcgcgcggca
     1021 cgcagggcgc gtcatcacct acggcatcga ccacccggcg gacgtgagcg cgaccgacat
     1081 cgagcgcgca gggctagggc tgacgcggtt ccgtttgcgc acgccgctgg gcgaagcgaa
     1141 ggtcgagctg ccgatgcccg gtcggcacaa cctctcgaac gcgctcgcgg cggcggcggt
     1201 cgccacctcg ttcgacctcg cgcccggcac catcgccgaa gcgttatcga ccgccgcgcc
     1261 gtcgcagatg cgcggcgagg tcttgagttt cgccgagggc ttcacggtcg tggacgactc
     1321 gtacaactcg aacccgcgct cgctgctggc gatggccgag gcggtggcgc agggcggcga
     1381 cgaagtgaag cggcgcgtca tcgtcgcggg cgagatgttg gaactcggcg ccgagtcggc
     1441 ggcgctccac cgcgaggcgg ggcgcgacat cgcgggactc ggcgtggacg tggtgtgggg
     1501 cgtgcgcggc gacgcgcgcg agctggtcga aggcgcgcgc gaggcgggcc tgagcgaggg
     1561 cgcgaccgcg ttcttcgaga cctcggaaga ggcggcgcag gcgctcgcgg gcttcgtcag
     1621 ggcgggcgac ctcgtcctcg tcaaaggctc gcgcggcgtc cacatggaga gggtcgtcga
     1681 ggcgcttcgc gctaagtgca gaatgcagag cgcagaatga tgagtaaaga aaactccatc
     1741 tttcattcat cgttccgcac tcatcaccca tcattttcgt agatgatcta ctacatcttt
     1801 tacgagttgc tctaccggaa tctggcgggc gggtcggagt cttacttcgt gaaggcgctg
     1861 aacgtcttcc agtacgtcac gttccgcacg gcctactcga cgatcacggc gctgctgatc
     1921 tcgctcgtct tcggcgggcg cgtcatccgg gcgctcagga atttgaacgt cgggcagcag
     1981 atccgcgaag aggggccgca ggcgcacatg gcgaagcgcg gcacgccgac gatggggggc
     2041 gtgctggtca tcggctccgt gctcatctcg acgctgctgt gggcgcggct gtcgagcgag
     2101 tacgtgtgga tcatcctgat cgcgacgacg ctctttgccg ccatcggctt tctggacgac
     2161 tacgcgaagg tcgccaagaa gcagagcctc gggctgaagg ggcggcagaa gctcgtcggg
     2221 cagtttttga tcgcggcgtt cgtgtggggt tctttgtacc tgctgaccga gtacggctac
     2281 gcgaactaca cgtggcggct gagcctgccg ttcttcaagg acacggcgct gacgcgcatc
     2341 accgagatcg ggccgtggct ctacctgccg ttcatcgtca tcgtgctgac gggattttcg
     2401 aacgcggtga atttgacgga cgggctggac gggctggcca tcagcgtgac ggtcgtggcg
     2461 atggcgacgc tgacggcctt cacctacgtc agcggcgacg cgcgttgggc ggcctacgtc
     2521 gggcttgaac accagaagga catcggcgag ctgacggtct tctgcggggc gatggtcggc
     2581 gcgagtctcg gctttttgtg gttcaacgcg ccgcccgcag aggtcttcat gggcgacgtg
     2641 ggcagcctcg ccatcggcgg cgccatcggc accatcgggg tgctgacgaa gcaggagttc
     2701 atcctgattc tgatcggcgg cgtttacgtg cttgaggcgc tgtcggtgac gttgcaggtc
     2761 ggcttcttca agctgacgaa gcggacgcaa ggcacggggc ggcggctctt caagatgacg
     2821 ccgctccacc accacttcga gatgctcgac tggaaggagc cgaagatcgt cttccgcttc
     2881 ctcatcatcg cgatcctgtt cgcgctgttg agtctctcga cgttgaagct gcggtgaaaa
     2941 gaagggtgtt gggtgtcggg tttcaggtgt tgggtaaaac caaaagcctg aaagccgaaa
     3001 cccaacaccc aacacctagc acccgttctt cctatggatg tcaacggtaa aaaagtcctt
     3061 gtgatcggcg cggcgcggag cggcgtcgca gcgtcgcggt ttctggcggc gcgcggggcg
     3121 gtggtcgcgc tgaacgaccg caagccgttc gtggactggc cgaacgaggc gctgcgtctg
     3181 aagggcgagg gcgtggggct gctcgcgggc gaggtgcctt cgtggctgct cgatcaggtg
     3241 gagctggtgg tcttgagtcc gggcgtgccg acgaagagca tcacggcgcg ctacgcggat
     3301 cgcgcgggcg cggaggtgat cggcgaggtc gagctggcgt ggcggttcct gcgaggtcgc
     3361 gtggtcggca tcacggggac gaacgggaag acgacgacga cggcgctcgt aggccgcctg
     3421 ctcgaagagg ccggcctccc cgtgcaggtc gggggcaaca tcgggacgcc gctcatctcg
     3481 ctcgtcgagt cttcgcgcga ggacggttgg acggtggccg agttgtcgag ttatcagctc
     3541 gagacgatca aggagtttcg cccgacggtg gcgctcgtgc tgaacctgat gcccgaccac
     3601 atggatcgct acgacagtct ggcggactac ggcgcggcga agcaccgcat cttccgcaac
     3661 cagaccgcgg cggacgtggc cgttttgaac gcggacgacg agatcgtcgc ctcgtgggcg
     3721 cgcgggctga acgcgcacgt cgtgatgttc agcacggagc gcgagcttga agaggggctg
     3781 ttcctgcgcg gcgggcgcga gctggtggcg cgcacgctgg cgggcgagcg cgtgctgatg
     3841 acgcgcgaag agctacagct caagggcgtg cacaacgtgc agaacgtgct ggcggcgctg
     3901 gccgcgggac tcgcgtgcgg cgcgtcgccc gagtcgatgc gcgcgaccgt gcgaggcttc
     3961 gggccggtcg agcacaggct ggagcgcgtc gccgaggtcg gcggcgtgac gtactacaac
     4021 gactcgaagg cgacgaacgt ggacgcggcg gtgaaggccg tcgaggcgct ggcggacgaa
     4081 ccgggcgaga tcgtgctcat cctcggcggg cgcggcaaga acgcgccgta cgcgccgctc
     4141 gcgccgctgg tgcgcgggaa gggccgcaag ctggtcgtcc tcggcgaaga cgccgaccgc
     4201 atcgaggacg agttgaaagc tttcgcgccg gtcgcgcgcg cgaaggacat ggccgaggcc
     4261 gtgcggctcg cgcacgcggc ggcgcggccc ggcgacatcg tgctcttggc ccccgcgtgt
     4321 gcaagcttcg acatgttcac gggcttcgag caccgcggtc gcgtgttcaa ggaagaggtc
     4381 aaccgattgc ggactgcgga ttgcggattg cggaatgaaa aagcgaacgc gctttgattc
     4441 cacgaatccg cagtccgcag tccgcaatcc gcaatcgaaa tatggctcga aagttacaac
     4501 ccgacgagtg gctgttcgcg gtgacggtgg cgctggcgct gttcggcgtc atcatggtgt
     4561 acagcgcgtc ggcggtggtg gcggcggtgg gcgaggcgca cgggcagtat cactacgtcg
     4621 cgcggcaggg cgtgtggacg ttgatcgggc tgggggcgat gttgttcggc gcgcggctgg
     4681 actacgggtg gctgcggagc gaggtggtgg tctacgggct gctcgtgttg acggtggtgc
     4741 tgctggtggc ggtcttcgcg ttcccgccga tcaacggcgc gcggcgctgg atacggttcg
     4801 caggcttctc ggcgcagccg tcggagttgg cgaagctgac gctggcgatt ttcctcgcgc
     4861 gcctgctcga acggcgggcg ggcgacgagg ggaacttctg gcggacgttc gtgccgtgcg
     4921 tcgcggtggc gggcctgctc gtgctgctgg tcgtcgcgga gcctgacctc gggacggcga
     4981 tgatgctcgg cgtcatcgcg gtcgtcatcc tgttcacggc gggcgtgcgg ctgaagcaca
     5041 tggcgctggc ggcggcgccc gcgctcatcg ggttggcggg gctgctgctg ttcgtgtcgt
     5101 ggcgtttgaa gcggctggcg gtcttcctcg acccgtgggc cgacccgcag ggcgcgggtt
     5161 atcagatcat ccagtcgctg ctggcggtcg ggtcgggcgg ggtgaacggg ctggggttca
     5221 ccgaaggcag gcagaagctc ttcttcctgc cgttcgcgca ttcggatttc atcttcgcgg
     5281 tggtgagcga agagctaggg ctgctcggcg gtttgggcgt cgtcgccttg ttcgggctgt
     5341 tcctgtggcg cgggatgcgc gcggctttga acgcgccgga caggttcggg atgctgctgg
     5401 cgacgggcat cgtcgcaggc atcgtcgcgc aggctctttt caacatgagc gtggtgctgg
     5461 cgctggtgcc gacgaagggc atcccgctgc cgttcatctc gtacggcggc tcgtctttgg
     5521 tgccgacgct gttcgcggtc ggcgtgctct tgaacatctc gcagcacgcg gggcttggag
     5581 acaagggggc ttggcgcggc tggggcgcgg agacgcgggg acgcggggac gcgggcgcac
     5641 agtcgcgcac gaagggcgcg gcggcgtcgc cgaagcggcc cgtctcgacg ggggcgaccg
     5701 ccgccgcaca accctcgcgt ccccacgcct ccgcgcccca cgcggccaca ccgcgacgcg
     5761 cgttcgccgc ggcgctcagg cgcgcggggt gacggcgatg cgcgtgctca tcgcggcggg
     5821 cggcacgggc ggccacatct atccgggcat cgcggtggcg aaagaggtga tgcgccgcga
     5881 cccctcttcg gtcgtgaggt tcgtcgggac ggcgcgcggg ctggagacgc ggctggtgcc
     5941 gcaggcgggg ttcgaactct cgctcattga aagcacgggg ctggtcaaca tgggctggcg
     6001 cgagcgcgtg cgcggactcg cggtgctgcc gaaaagcctc gccgcggcgc ggcggttgat
     6061 acggagtttc agaccggaca tcgtcgtcgg cgcgggcggc tacgtgtcgg ggccggtgct
     6121 gctcacggcg gcgctgatgc gcctgccgac gctggtgatg gagtcgaacg ccgtgccggg
     6181 ttttacgaac cgccggctgg cgctcttcgt cagggcggcg gcggtctcgt tcgaggcggc
     6241 gctgccttac ttccgcggca agggcgtggt caccggcaac cccatccgcc gcgagttctt
     6301 tgaagtgcca gcgaaagagc gcgacccttc gcgcttctcg ctcctgatct tcggcggctc
     6361 gcagggcgcg cgcgcggtga acgaagcgat ggtcgccgcg ctcccgcacc tcgcggagca
     6421 ccgcgcggtg ctccgcgtca cgcaccagac cggcaagctc gacttcgaga aggtgcgcga
     6481 gggctaccgc gcggcgggct ggggcgagac ggcggacgtg cgcgagtaca tcgacgacat
     6541 ggtcgcggcc ttcgccgcgg cagacctcgt cgtctcgcgc gcgggcgcga cgacgagctt
     6601 cgagctgatg gccgcgggcc gcgccgccgt gatggtgccg ctccccggcc agctcgaaca
     6661 gcgccgcaac gcggaggcca tgcaggacgc gggcgccgcc cgcatgatct tgcaggaaga
     6721 gctttccggc gaacggctcg cgcgcgaaat cgccgcgctc gtgaaggacc cggagcaact
     6781 gacgcgcatg gggcgcgcca gccgcaaact cgcccgcggc gacgcggcgg cggcgacggt
     6841 ggatttgatg gagaagctag taggcagcag gcagcaggca gtaggcagtt gaaagaaagg
     6901 cagaaggcag aacgcagcgg tagtggaaac aagaatcgag agagcgatgt tcgtcaacgc
     6961 acagccgacg attgaacgcg aaacaatgca ggaggtattt gctgcctact gcctactgcc
     7021 taccgcctac tgaattttta tgtttcgtcg catccagcac gtccacctcg tcggcatcgg
     7081 cggcatcggc atgtcgggca tcggcgaggt gcttttgaac atggggttcc gcgtgtcggg
     7141 gtcggacttg cggcgctccg aggtgacgaa ccggctggaa aagttgggcg cgacgatctt
     7201 cgaaggccac gcggcggaga acatgacggg cgcgccgcac gtcgtcgtcc gctcgacggc
     7261 ggtgcgcgac gacaaccccg aggtcgtcga agcgcggcgg cgctcgatcc ccgtcatcgc
     7321 gcgcgcagag atgctggccg agctgatgcg cctgaagccg cacacggtcg cggtcgcagg
     7381 ctcgcacggg aagacgacga cgacctcgat gatcgcgacc gtgctggcgc acgccaacct
     7441 cgacccgacg gtggtggtgg gcggcgtggt cggcgcgatg ggttcgaacg cgcgggtggg
     7501 gaagagcgac ttgatggtgg tcgaggcgga cgagagcgac cggtcgttcc tgatgctgac
     7561 gccgacgttc gccgtggtga cgaacatcga ccgcgagcac atggactatt acgcggacat
     7621 gatggacgtg cgcaagtgct tcacggactt cgtcaacaag gtgccgttct acggcgcgtc
     7681 cgtgctgtgc ctcgacgacc cgcacgtgca ggcggtgatc ccggaggtcg tccgccgccg
     7741 catcacttat ggcttgagcg cgcaggccga catctctgcg cgcgaggtgc gctacaacca
     7801 gcagttcggc tcgacgttcc aggtgcggca gttcggcgag acgttgggcg aggtgacgtt
     7861 gaacgtgccg ggcctgcaca acgtctacaa cgcgctcgcg gcgacggcca tcggcctcga
     7921 actcgaagtc gagttcgacg tgatcgcggg cgcgctcgcg gacttccgcg gcgtcaaccg
     7981 ccgcttccag ttcaagggcg aggaggcggg cgtcatcgtc gtggacgact acggccacca
     8041 cccgaccgag attcgcgcga cgctcgtcgc cgccaaactc ggcacgacgg gcaggcgcat
     8101 ggtcgtcctc ttccagccgc accgctacac gcggacgcag gacttgatgg acgagttcgc
     8161 gcgctcgttc aacaacgccg acgtgctgat ggtgaccgac atctacgccg cgagcgaaga
     8221 ccccatcgag ggcgtcaacg ccgaggcgct ggtcgaggcc gtcaagcgct acggccacaa
     8281 ggacgcgcgc cacgtcggct cggtcgagga cgcgacgcgg gcgctgctgg aagaagtgcg
     8341 cgagggcgac atggtcatca cgctcggcgc gggcaacgtc tatcgcgtca gcgaggagtt
     8401 gccggggctg ttgaaagaga gggcgcaagg ttgatgacgg cggcggcccc ccgcatgttt
     8461 gttatgacgg aagctgaagg cacagccgac actgagaaac gcgacgcgct tgtggaagag
     8521 atcgcggcgc ggctcggcgt gcgcgccgac cgcgggcggc ggttcgcgga gttgacttct
     8581 ttgcgcgtgg gcggcgcgat cgattgggtg ctgttgccgg agaccgagga gcaggcggca
     8641 ggcgtggtgg gcgcgctcga agcggcgggg gttgcgtggc gcgtgctcgg cgcggggagc
     8701 aacgtgctgg cggacgaccg cgagcaccgt tacgtcgtcg tctcgatgcg cgaggtcaaa
     8761 ggcgaggcgc ggttcgcggg cgagcgcgtg tctgtgagcg cggggttctc tctgccgcgg
     8821 ctgtgcgtcg aggcggcgcg gcagggcttg tcgggcatcg agggcttggg cggcataccg
     8881 ggcacggtgg gcggcgcgct gtggatgaac gcgggcgcgt acgggcacga gatcggcgag
     8941 gtggtcgagt cggtgagggt cgcgtgcggc ggcgcggtcg tcgaggtgcc ggggcgcgag
     9001 gtgaaatgga actaccgcca cacgtcattc aaagagggcg agctgctctt gggctgcacg
     9061 ctgaagttga agccggacga ccccgcggag atcaaggcgc ggatggaaga ggccaagcag
     9121 aaacgtttcg ccacgcagcc gcacggcgcg cgcagcgcgg gctgcttctt caagaacccg
     9181 ccggggtcgg gcctgagcac ggggaagatg atcgacgaga tgggcatgaa gggagagcga
     9241 aggggcggcg ccgtcgtctc gccggtgcac gcgaacttca tcgtgaccga gggcgagggc
     9301 gcgagtgcgg cggacgcgct cgcgctcgcc gaagagatac gcgagcggtt ccggcgcgag
     9361 cacaacgtcg agctcgagta cgaggtcgag ctgtggggcg cggacagaga gttcagggac
     9421 gagaagggga gggcggagga tgaaggagcc gctgcagtga gtcgggcgag ggacgaagga
     9481 gccggcggcg tgag
//
LOCUS       DBNGOH010000442         2063 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_5016092, whole genome shotgun sequence.
ACCESSION   DBNGOH010000442 DBNGOH010000000
VERSION     DBNGOH010000442.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2063)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2063)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2063
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_5016092"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(212..1885)
                     /locus_tag="AC159P_08785"
     CDS             complement(212..1885)
                     /locus_tag="AC159P_08785"
                     /inference="COORDINATES: protein motif:HMM:NF019604.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR3695587.1"
                     /translation="MRVSTLSKNGDGRRKRICILGGGFAGVYTAQYLEKAPGRRDDFE
                     IVLVNKENYFVFQPMLAEVVSGAIGITDAVSPIRRLLPRTDLHVREIESVDLAARTVT
                     TAPGFMPHPHVIHFDHLVLALGGVTDFRGLRGLPEHALPFKNLGDALALRNHVIHALE
                     EAAIEHEDACLRRRLLTFVVAGGGFSGVEVVAELNDFVREAARSYRHIDPAEIRVVLL
                     HGRERILPEVTEKLGLFAQKILRRRGVEIHLDTRLRAATGNEAILEDGAHIPTRTLVS
                     TVPSSPHPIIQSLDLPRDKSGRLTANAFLEVRGAENVWALGDCALVPLAGGQGSSPPT
                     AQHAIRQARVAAHNIVADIRGGEKRTFDFKDLGKMGALGHRSAVAEVMGVPVSGLVAW
                     LMWRAVYLMKLPGWGRRLKVAASWALEVLLPIELAQLKLNPTEGVHREHFEPGQEVFC
                     QGDLGDHVYIIVGGKAEVLREEEGRSIKLAELGAGEYFGEMALINNTTRNATVRCVEP
                     LDVVSLPKREFMMVTTSLPDARRRFEEVVEQRRRATTLALGREVEREVV"
BASE COUNT          335 a          721 c          654 g          353 t
ORIGIN      
        1 ctgcccgctg acatccgctt acttcaacgt cgagagcgcc gtggccgcgg gcagggacaa
       61 gccctgcccc taccgtcatc cctgacatgg taatttcagc cgcaaacctc tctaacgaaa
      121 tcttatcaga ggcggcatcc cgcgcaggcc gacgccgagg ggcgccgcac atgaaatctt
      181 ttcccgcccc gcgcctgcca ttcttattac ttcacacgac ctcgcgctcg acctcgcgac
      241 cgagcgccag cgtcgtcgcc ctccgccgct gctccacgac ctcctcgaat ctacgcctcg
      301 cgtcgggcag gctcgtcgtc accatcatga actcgcgctt cggcaggctg acgacatcca
      361 gcggctcgac gcagcgcacg gtcgcgttgc gcgtcgtgtt gttgatcaaa gccatctcgc
      421 cgaaatactc gccggccccc agctcggcaa gcttgatgct ccgaccttct tcctcgcgca
      481 gcacctcggc cttgccgccg acgatgatgt agacgtggtc gccgaggtcg ccctgacaga
      541 agacttcctg ccccggctcg aagtgctctc tgtggacgcc ctccgtgggg ttcagtttca
      601 gttgcgccag ttcgatcggc agcaacacct ccagcgccca agaggccgcg accttcagac
      661 ggcggcccca gcccggcagc ttcatcagat aaaccgcgcg ccacatcagc cacgcgacga
      721 ggccggagac gggcacgccc atgacctcgg ccacagcgga gcgatggccg agcgccccca
      781 tcttgccgag gtctttgaag tcgaaagtcc gtttctcgcc gccgcggatg tcggccacga
      841 tgttgtgcgc agcgacgcgc gcctgtcgga tggcgtgctg cgccgtcggc gggctgctgc
      901 cctgccctcc ggccaaaggg acgagcgcgc agtcgccgag cgcccagacg ttctccgcgc
      961 cccggacttc gaggaacgcg ttcgccgtca ggcgaccgga cttgtcgcgc ggcaggtcga
     1021 ggctctggat gatcgggtgt ggcgaagagg gcacggtgga gacgagcgtc ctcgtcggga
     1081 tgtgcgcgcc gtcttcgaga atcgcctcgt tccccgtcgc cgcccgcagc cgcgtgtcca
     1141 gatgaatctc gacgccgcgg cggcgcaaga ttttctgcgc gaagagtccg agcttctccg
     1201 tcacctcggg caggatacgc tcgcgcccgt gcagcagcac gacgcgtatc tccgccgggt
     1261 cgatgtggcg gtagctgcga gccgcctcgc ggacgaagtc gttgagttcg gccaccacct
     1321 cgacgcccga gaagccgccg cccgcgacga cgaaggtgag cagacggcgg cgcaagcaag
     1381 cgtcctcgtg ctcgatggcg gcctcttcga gcgcgtgaat cacgtggttg cgcaaggcga
     1441 gggcgtcgcc cagattcttg aaggggaggg cgtgctcggg caggccgcgc aggccgcgaa
     1501 agtccgtgac gccgccgagc gcgagcacca gatgatcgaa gtggatgacg tgcgggtgcg
     1561 gcatgaagcc cggcgcggtc gtcacggtgc gcgccgcaag gtctaccgac tcgatctcgc
     1621 gcacgtgcaa gtccgtgcgc ggcagcagcc ggcggatggg gctgaccgca tcggtgatgc
     1681 cgatggcgcc ggacacgacc tcggccagca tcggctggaa gacgaagtag ttctccttgt
     1741 tgacgaggac gatctcgaag tcgtcgcgcc gccccggagc cttctccaga tactgcgccg
     1801 tgtagacccc ggcgaagcct ccgccgagga tgcagatacg cttcctgcga ccgtcaccgt
     1861 tcttcgatag cgttgacact ctcatgggtt tcattccttg ttggcgccgt caggcgactc
     1921 gataaattct gatgggcgcc tcgcgcccct tgaccggcac gtgccccatc gggagcgcgc
     1981 cggccagccc gtcttcgcgc gcggcctccc acaccacttc cgagagcagg agttgggagc
     2041 cgaactgttt gttcagttgc tcg
//
LOCUS       DBNGOH010000443         7743 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_5016093, whole genome shotgun sequence.
ACCESSION   DBNGOH010000443 DBNGOH010000000
VERSION     DBNGOH010000443.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7743)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7743)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7743
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_5016093"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(97..3060)
                     /locus_tag="AC159P_08790"
     CDS             complement(97..3060)
                     /locus_tag="AC159P_08790"
                     /inference="COORDINATES: protein motif:HMM:NF051698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR3695588.1"
                     /translation="MLARFHHIRRINFFTAACLLLAGALTASAQEGTATLRGTVLDAN
                     GAAVPGAQVSIANQETGLNRRTATTGEDGEYVFTSLTPGLYRIMVEAAGFKRSLKENV
                     KLDVGEEQEFKVGLEVGGADETVTVSADEPLIQTTSKEIGGHIGQRELTELPSVNRNF
                     IGFVGLLPGVVPNISTESFGSDSVSVNGQDPRFNNYMLDGSNNNDDVIGQRAGAQART
                     ALEAVQEFQVLTNQFDAEYGRTSGGIINAITKSGTNEFHGSVFGFFQDSSLNAVSRFA
                     ELNRLEDPEASYKQFGGTIGGPIKKNIAHFFFSYERTNIDEGVVVDIPARPSLNASTT
                     EQTRADNTTLRFDMQPAQKHQIAVRWLREKSPQLNQIIVAFNRPVSLEASREESDIDQ
                     TVVGSWTYTLTPLLLNDMRVSFTREDVAFANPGFNGGSSMAELPPTLVFNSFVTQQSN
                     IAQARVNDAYRLADVVSRVQGEHTFKFGLDYNYVTAANLTEDSLNGVFMFPTDLPFDA
                     ADPRTYPERLQIRVGGPQTFTVINHNTSFFAQDQWRVSPKLTLNLGLRYDDETISDDQ
                     NNLSPRIGFAYDPTGNGKTVVRGGYGHFYQNTPFELITAFRTAGPFSNSFVRNFPLNA
                     ADPGPRAGRLPTDPTLANGPVVNRDLINSIVGTGTLLGNPTPVVDNSTRRMPYVRSFS
                     VGLQRELIPNLAVTADYIHQTGVDQLMTVNLNPGVRATTASTAAISRRFSTLGAVIQN
                     STLPVDTSLFADLNFQTLGVSGVTTRVNEGRTKYDALQLSLDKRYSRGFQLKASYTLS
                     RGRGNTSGDGVPTANFQTQDGFNLDLNEGPTGFDRRHNFVFSGLWRVPRTRGFVVSTI
                     VRALSGTPFTLQDTRVDVDRNGILFDPLPAGTFNASRTFPNGETLGFDVENEGGRNGA
                     RFPGFFGVDLRLAYKFNFTEHVNAGFTFEAFNLTNRTNYDELFITGDTSSPNFLIPLA
                     AKPRRTLQLGFRLGF"
     gene            3749..4765
                     /locus_tag="AC159P_08795"
     CDS             3749..4765
                     /locus_tag="AC159P_08795"
                     /inference="COORDINATES: protein motif:HMM:NF012894.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HIR3695589.1"
                     /translation="MRTNLKRVNFAALTFALALAMAPAILAQDTSSSNSRTVATGQKM
                     KLKGVVTRRDADTFVVQDANGVATTVTLTDRTSVKTKGGFLRSGTNYGVTAILRGLNL
                     EVEGRGDGNTLVAEKIRFNETDLRTARTVEANVVPVENRVGAAEGRIGQVEQNAQRLS
                     GQLDELAAISNAARGGAKAAQETADAAVAGVTATNERISALDDYQVQNTTSVLFRVGS
                     AVLRPEAKTQLDGVAQQALQAKGYVLEVSGFASSDGSTEANRRLSQRRADAVIRYLVE
                     NHNIPLRRIITPYGYGELQPIAENDTREGREQNRRVEVKVLVSRGIIQSAPAMNRPST
                     DNPE"
     gene            4950..6170
                     /locus_tag="AC159P_08800"
     CDS             4950..6170
                     /locus_tag="AC159P_08800"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HIR3695590.1"
                     /translation="MRSNASLRDSQGRFKGAAGLFIALGLLASLLPAGLADAQTRRTR
                     PRRVSTPFAQTTPTPTPSPIVVPQLPPAQRTQTPPTTAPQQQPPQQRPTPAPTPEAKP
                     GEALADDDDVVRVTSNLVVVPVSVVDAKGEAVQGLTREDFRLEEDGRAQELASLGNAD
                     QVPLDIAILIDTSSSVKERFDFEKQTAAGFLKTVLRPGDKAAVYAIGDKPGFVQALAT
                     ADEAGGKLLAIPPPPEHRFTAYFDTVIEAARYLDKNAPGNHRRVIVALTDGDDTARIL
                     AASLVDSQGKQINMLNESQQQVLREVQRADAVFYSINPSGQTMHLNVRTARSQEGMQQ
                     LAAATGGNAFVPTRLEDLPAVFRQIANELRAQYLIQYQSNGEAQPGKFIKINVTTPAR
                     AGLTIRARQGYYKK"
     gene            complement(6338..6856)
                     /locus_tag="AC159P_08805"
     CDS             complement(6338..6856)
                     /locus_tag="AC159P_08805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695591.1"
                     /translation="MKSLIAATTALMLLLSAAPAAFGAQTRTRRSTTPQRRRAPAKPA
                     TSSLDATRTNAVRLQLASLNKDMTRFLYVYGRLSKDLELTGSQTESADVTNKTRAGLI
                     ESLKTMSNRLDQLEGQVRFTPGLERHYRSLQGVSARAAQAEANAAAGRFDAAGRILVE
                     VAATLTDVLLEM"
     gene            7032..7712
                     /gene="lipB"
                     /locus_tag="AC159P_08810"
     CDS             7032..7712
                     /gene="lipB"
                     /locus_tag="AC159P_08810"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HIR3695592.1"
                     /translation="MSTALRTKPAGHPRPLRVRRLGRLEYAAAYRVQKEAEAAVKARE
                     CPDTLLLVEHPHVLTIGRRAAPSAILATPEHLAARGVAVHEADRGGKMTYHGLGQLVG
                     YPVINLSPDREDVHAYVRDLEEALMRALADFGVEGFRVKGLTGVHTARGKVAAIGVHI
                     SRWVTTHGFALNVNTDLSFFDLIVACEGEPVTSLRELLGREVPLHEVEDRVVARFAEV
                     FDMTTEEP"
BASE COUNT         1316 a         2514 c         2645 g         1268 t
ORIGIN      
        1 gattgtcttc tctgtgccct ctgtgtgtcc tccgtgccct ccgtgttgaa acgaaccctc
       61 cctgcgctcg accctcgctc gcagcagagg ccgcggctag aagccgaggc ggaagccgag
      121 ttgcagcgtg cgcctcggct tggcggcgag ggggatgagg aagttggggc ttgaggtgtc
      181 gccggtgatg aacagctcgt cgtagttcgt ccggttcgtg aggttgaacg cctcgaaggt
      241 gaagccggcg ttgacgtgct cggtgaagtt gaacttgtag gcgagccgca gatcgacgcc
      301 gaagaagccg gggaagcggg cgccgttgcg cccgccttcg ttctcgacat cgaagccgag
      361 cgtctcgccg ttcgggaagg tgcgcgaggc gttgaaggtg cccgccggga gcgggtcgaa
      421 gaggatgccg ttgcggtcaa cgtccacgcg cgtgtcttgc agcgtgaagg gcgtgcccga
      481 gagcgcgcgg acgatggtgc tgacgacgaa gccgcgcgtc cggggcacgc gccacagccc
      541 gctgaagacg aagttgtggc ggcggtcgaa gccggtcggc ccctcgttga gatcaaggtt
      601 gaaaccatcc tgcgtctgga agttggcggt cggcacgccg tcgccgctcg tgttgccgcg
      661 cccgcgcgag agcgtgtacg aagctttgag ctggaagccg cgcgaatagc gcttgtcgag
      721 cgagagttgc agcgcgtcgt acttcgtgcg cccctcgttg acgcgcgtcg tcacgcccga
      781 gacgccgagc gtctggaagt tgaggtcggc gaagagcgac gtgtccacgg ggagcgtgct
      841 gttctggatg acggcgccga gcgtcgagaa gcggcggctg atggcggcgg tcgaggccgt
      901 cgtcgcgcgc acgccggggt tgaggttgac ggtcatcaac tgatcgacgc ccgtctggtg
      961 gatgtagtca gccgtgacgg cgaggttcgg gatgagttcg cgttgcaggc cgacgctgaa
     1021 cgagcggacg tagggcatcc ggcgcgtcga gttatcgacg acgggcgtcg ggttgcccag
     1081 cagcgtgccg gtgccgacga tggagttgat gaggtcgcgg ttgacgaccg ggccgttcgc
     1141 gagcgtcggg tcggtcggca gcctcccggc gcgcgggccg gggtcggcgg cgttcagcgg
     1201 gaagttgcgg acgaaggagt tggagaacgg ccccgccgtg cggaaggccg tgatcagctc
     1261 gaacggcgtg ttctggtaga agtggccgta gccgccgcga acgaccgttt tgccgttgcc
     1321 agtcgggtcg taggcgaagc cgatgcgcgg cgaaagattg ttctggtcgt cggagattgt
     1381 ctcgtcgtcg tagcgcaggc cgaggttcaa ggtgagcttc ggcgacaccc tccactgatc
     1441 ctgcgcgaag aagctggtgt tgtggttgat gacggtgaag gtttgcgggc cgccgacgcg
     1501 aatctgcaag cgctcggggt aggtgcgcgg gtcggcggcg tcgaacggca ggtcggtcgg
     1561 gaacatgaag acgccgttca gagagtcctc ggtcagattc gccgccgtga cgtagttgta
     1621 gtcgaggccg aacttgaagg tgtgctcgcc ctgcacccgc gagaccacgt cggcgaggcg
     1681 gtaggcgtcg ttgacgcgcg cctgcgcgat gttgctctgc tgcgtgacga acgagttgaa
     1741 gacgagcgtc ggcggcagct cggccatcga gctgccgccg ttgaagccgg gattggcgaa
     1801 ggccacgtcc tcgcgcgtga acgagacgcg catgtcgttg agcaagagcg gcgtcagcgt
     1861 gtacgtccaa ctgccgacca cggtctggtc gatgtccgac tcttcgcgcg aggcttcgag
     1921 cgagacgggg cggttgaagg cgacgatgat ctggttgagc tgcggcgact tctcgcggag
     1981 ccagcggacg gcgatctggt gtttctgcgc gggctgcatg tcgaacctga gcgtcgtgtt
     2041 gtcggcgcgc gtctgctcgg tggtcgaggc gttgagcgag ggccgcgcgg ggatgtccac
     2101 gaccacgccc tcgtcgatgt tggtgcgctc gtaggagaag aagaagtgcg cgatgttctt
     2161 cttgatgggg ccgccgatgg tgccgccgaa ctgcttgtag ctggcctccg ggtcttcgag
     2221 ccggttcagt tcggcgaagc ggctgacggc gttcagcgag ctgtcctgga agaagccgaa
     2281 gacggagccg tggaactcgt tcgtgcccga cttggtgatg gcgttgatga tgccgccgga
     2341 ggtgcgcccg tactctgcgt cgaactggtt ggtgagcacc tggaactcct gcaccgcttc
     2401 gagcgcggtg cgcgcctgcg cgcccgcgcg ctggccgatc acgtcgtcgt tgttgttcga
     2461 cccgtcgagc atgtagttgt tgaagcgcgg gtcttgcccg ttgacgctga ccgagtcgga
     2521 gccgaacgac tcggtgctga tgttggggac gacgccgggc agcagcccga cgaagccgat
     2581 gaagttgcgg ttgaccgaag gcagctcggt cagctcgcgc tggccgatgt gcccgccgat
     2641 ctccttcgag gtggtctgga tcaaaggctc gtccgccgag acggtgacgg tctcgtcggc
     2701 gcccccgact tcgaggccga ccttgaactc ctgctcctcg cccacgtcga gcttgacgtt
     2761 ctccttgagc gaccgcttga acccggcagc ctcgaccatg atgcgataga ggccgggcgt
     2821 gagcgaggtg aagacgtact cgccgtcctc gccggtcgtc gccgtgcgcc ggttgaggcc
     2881 cgtctcctga ttggcgatgg agacctgcgc gccgggcacg gcggccccgt tcgcgtcgag
     2941 cacggtgccc ctcaacgtgg ccgtcccttc ctgtgcagag gccgtgagcg cgcccgccag
     3001 gagcaggcag gccgccgtga aaaagttaat gcgtctgatg tgatggaacc ttgcgagcat
     3061 gggctactcc tttcgttgcg aggaagattt aggcgggctg gtctggtagg ggcgaacccg
     3121 tcgtggagaa tgcgtgggcg atgtttgctg tgcgacatcg ctgcgacacc tccgaaagac
     3181 ccgcgggccg gggtcacagt aaagacccgg cctcgccact ggccgcggcg ccattggggc
     3241 ttgcgacgac cgcgggggaa tgttgttgtg cgtgagaatc cggcaacgcg gatgccacat
     3301 cttaacagat gtcattcccg ctgaatatgc cgaagcgcgg acggcctcgc gcctcggcca
     3361 tccgaaattt cgtcgccgcg tcttctgttt ttcggataac ctcgggccgc actcgcgccc
     3421 gaaattgttg cggctcaatg gcgattcgat gataaatagc ttcggcgcaa cgaaatccgt
     3481 ggtttctcca tcacaacccc ttgacttggt ggcggtgttc attaagaatc cgggctaggt
     3541 tcaagcaaca cgacaacttg tcctcgcacg gcaacagtca tgggcgggct tgctttatca
     3601 ggcgctcggg cgatacgcgt gcgacgaacc gtgaaagctc cttgtggggg cacgcgagct
     3661 ggaagggttc gccgctctag agacgctgaa gtccggaggc gcgcgaagct tcccgacaag
     3721 actttcgcat gatggagaat aaacgacgat gagaacgaat ttgaaaaggg ttaatttcgc
     3781 ggcgctgact ttcgcgctgg cgctcgcgat ggcgccggcc atcctcgctc aggacaccag
     3841 cagctccaac tcgcgcaccg tcgccacggg gcagaagatg aagctcaagg gcgtcgtcac
     3901 gcggcgcgac gccgacacct tcgtcgtgca ggacgccaac ggcgtcgcga cgaccgtcac
     3961 gctcaccgac cgcaccagcg tcaagaccaa gggcggcttc cttcgcagcg gcaccaacta
     4021 cggcgtgacc gccatcctgc gcggcctcaa ccttgaggtc gaggggcgcg gcgacggcaa
     4081 cacgctggtc gccgagaaga tccgcttcaa cgaaacagac ctgcgcacgg cgcgcaccgt
     4141 cgaggccaac gtcgtccccg tggagaaccg cgtcggcgcg gctgaggggc gcatcggaca
     4201 ggtcgagcag aacgcgcagc gcctgtcggg tcagttggac gagctggccg ccatctcgaa
     4261 cgcggcacgc ggcggcgcga aggccgcgca ggaaacggcg gacgccgcgg tcgccggcgt
     4321 caccgccacc aacgagcgca tctcggcgct cgacgactac caggtgcaga acacgacgag
     4381 cgtgctcttc cgcgtcggca gcgccgtctt gaggcccgaa gccaagacgc agctcgacgg
     4441 ggtcgcgcag caggcgcttc aggctaaggg ctacgtcctc gaagtctcgg gcttcgccag
     4501 ctccgacggc tcgaccgagg cgaaccgccg cctctcgcag cgccgtgcgg acgccgtcat
     4561 ccgctacctc gtcgagaacc acaacatccc gctgcgccgc atcatcacgc cgtacggcta
     4621 cggcgagttg cagcccatcg cggagaacga cacgcgcgag gggcgcgagc agaaccgccg
     4681 cgtcgaggtg aaggtgctcg tcagccgcgg catcatccag agcgcgcccg cgatgaaccg
     4741 gcccagcacg gacaaccccg agtaaggccg gcccgctcga acacggacag caacaggcgg
     4801 cgggacggct cacgcttccc gccgcctgtc gcatttcgcc gcgaggcaca ggcccccgcg
     4861 cccaaccacc ggacaccccc tcggcatcca cacacagcga gcgtaaactg tgaccggcgg
     4921 acacacacgc cgcgacggag ccaaatttta tgagaagcaa tgccagcctg agagattcgc
     4981 aaggacgttt caagggggcc gccggactct tcatcgcgct cggcctgctc gcgtctctgc
     5041 tgcccgccgg actcgcggac gcgcagacgc gccgcacgcg cccgcgcagg gtctcgacgc
     5101 ccttcgcgca gaccacgccg acgcccacgc cttcgcccat cgtcgtcccg caactgccgc
     5161 ccgcgcagag gacgcagacg ccgccgacga ccgcgccgca gcaacagccg ccgcaacaga
     5221 ggccgacgcc cgcgccgacg cccgaagcca aacccggcga ggcgctcgcc gacgacgacg
     5281 acgtggtgcg cgtgacctcg aaccttgtgg tcgtgcccgt ctcggtggtg gacgcgaagg
     5341 gcgaggccgt gcagggtttg acgcgtgaag acttccggct cgaagaggac gggcgcgcgc
     5401 aggagttggc ctcgctcggc aatgccgatc aggtgccgct cgacatcgcc atcctgatcg
     5461 acacttcgag cagcgtcaaa gagcgcttcg acttcgagaa gcagaccgcc gccggcttcc
     5521 tgaagacggt cttgcggccc ggcgacaagg ccgccgtcta cgccatcggc gacaagcccg
     5581 gcttcgtcca ggcgctcgcc acagcggacg aggcgggcgg caaactgctc gccatcccgc
     5641 cgccgcccga gcatcgcttc acggcctact tcgacaccgt catcgaggcc gcgcgctatc
     5701 tcgacaagaa cgcgcccggc aaccaccgcc gcgtcatcgt cgcgctcacc gacggcgacg
     5761 acaccgcgcg catcctcgcc gcctcgctcg tcgattcgca gggcaagcag atcaacatgc
     5821 tcaacgagtc gcagcagcag gtcttgcgcg aggtgcagcg cgccgacgcc gtcttctact
     5881 cgatcaaccc gagcggccag acgatgcacc tcaacgtccg caccgcgcgc tcgcaggagg
     5941 gcatgcagca actcgccgcc gccaccggcg gcaacgcctt cgtccccacg cggctcgaag
     6001 acctgcccgc cgtcttccgc cagatcgcca acgagctgcg cgcgcaatac cttatccagt
     6061 accagtccaa cggcgaggcc cagcccggca agttcatcaa gatcaacgtc accacgcccg
     6121 cccgcgccgg cctgaccatc cgcgcgcgtc agggctatta caagaaatag cgggcagcag
     6181 acagcaggca gcgggcagtt aaagacagaa gaggcttgag acggagcgtc tcaagcctct
     6241 tgcttctttg attgaagcgt gaagacacct gagaggcaaa gtcccaaacg tcttcgctct
     6301 tctgttctta ctgcctgctg cctgctgccc gctgttctta catttcgagc agcacgtcgg
     6361 tcagcgtcgc ggcgacttcg acgaggatgc ggcccgcggc gtcgaagcgg cccgcggcgg
     6421 cgttggcctc ggcctgcgcg gcgcgggccg agacgccttg cagcgagcgg tagtggcgtt
     6481 cgaggccggg cgtgaagcgc acctgccctt cgagctggtc gaggcggttg ctcatcgtct
     6541 tcaggctctc gatcagcccg gcgcgcgtct tgttcgtcac gtccgccgac tcggtctgcg
     6601 agccggtcag ctcaaggtct ttcgagaggc gaccgtagac gtagaggaag cgcgtcatgt
     6661 ctttgttgag cgaggcgagt tgcagccgca cggcgttcgt ccgcgtcgcg tcgagcgaag
     6721 aggtcgcggg cttcgcgggc gcgcgcctcc tctgcggggt cgtgctgcgg cgcgtgcgcg
     6781 tctgcgcgcc gaaggcggcg ggcgcggcgg agagcaggag catgagagcg gtcgtggcag
     6841 cgatcagaga tttcacagag aaccctcctt agaaactgtt ggggccgtcc ggccatgaac
     6901 ttcacgcggg agcttttgcc cccagactat tccccttttg ccgcgcgagg tcaagcggcg
     6961 aatcccgttc ggggagtacc cgccccgcaa ctcgtcccgg cgaggcgtgc gctgctacaa
     7021 tgccgactcg aatgtcaaca gcgcttcgga cgaaacctgc gggccacccg cgaccgctca
     7081 gagtccgccg cctcgggagg ctggagtacg ccgcggccta ccgcgtgcag aaagaggccg
     7141 aggccgccgt caaggcgcgc gagtgcccgg acactctact gctcgtcgag cacccgcacg
     7201 tgctgaccat cgggcgccgc gccgcgccgt cggccatcct cgccacgccc gagcacctgg
     7261 ccgcgcgcgg cgtcgccgtc cacgaggccg accgcggcgg caagatgacc taccacggcc
     7321 tcggccagct cgtcggctac cccgtcatca atctgagtcc cgaccgcgag gacgtgcacg
     7381 cctacgtgcg cgatctggaa gaggcgctga tgcgcgcgct cgcggacttc ggcgtcgagg
     7441 gcttccgggt caagggcttg accggcgtcc acaccgcgcg cggcaaggtc gccgccatcg
     7501 gcgtccacat ctcgcgctgg gtgacgacgc acggcttcgc gctcaacgtc aacaccgacc
     7561 tcagcttctt cgacctcatc gtggcttgcg agggcgaacc cgtcacctct ctgcgggaac
     7621 tgctcgggcg cgaagtcccc ttgcacgagg tcgaagaccg cgtcgtcgcc cgcttcgccg
     7681 aggtgttcga catgacgacc gaagagccgt gaacggtaaa ccgtaaaccg tgaccaagat
     7741 gaa
//
LOCUS       DBNGOH010000444         8549 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_985053, whole genome shotgun sequence.
ACCESSION   DBNGOH010000444 DBNGOH010000000
VERSION     DBNGOH010000444.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8549)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8549)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8549
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_985053"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..531)
                     /locus_tag="AC159P_08815"
     CDS             complement(<1..531)
                     /locus_tag="AC159P_08815"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF017864.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HIR3695593.1"
                     /translation="MESDNPKNEVPEAAPLVEVRRGAITESRHRGHIVAVDGEGAVVA
                     HLGTPHTVTFLRSSAKPHQTIPLLVSGAAERFQFTDREIAIACGSHSGQPVHAETVAE
                     MLRKIGLGADALRCGVHEPFDKQTAEEMRERGEQPSALHNNCSGKHAGMLALAVHLGA
                     PTETYDQPDNPVQLAAV"
     gene            complement(721..1059)
                     /locus_tag="AC159P_08820"
     CDS             complement(721..1059)
                     /locus_tag="AC159P_08820"
                     /inference="COORDINATES: protein motif:HMM:NF018437.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3D domain-containing protein"
                     /protein_id="HIR3695594.1"
                     /translation="MSFTATAYSLRGRTASGKPVARGLIAADRRVLPIGTRVRLEAGP
                     YSGEYTVADTGGAVRGRKIDIWMPSTREAMRFGRRPVKLTVLTRTRPRAKGKATQPVN
                     TTGKASQTVK"
     gene            complement(1695..2579)
                     /gene="dapF"
                     /locus_tag="AC159P_08825"
     CDS             complement(1695..2579)
                     /gene="dapF"
                     /locus_tag="AC159P_08825"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HIR3695595.1"
                     /translation="MRFIKFHGYGNDYLVFEEAELAGVASPGEFARRVCDRHFGAGGD
                     GIAIVGRLAAGADADFSLRIFNPDGGEAAMSGNGSRCAAAYLHYRGLWTDEELRFRTR
                     AGVKRYRLRERTGEGAFRFEAEIGRPLFDSASIPMRTPQPLAEVRDQPLALPGGETVR
                     VTAMQMCNPNCCLFAEDLERTDWRRLGRLIESHADFPERTNVEFIRVADRRNIEARVW
                     ERGVGETLSSGTGACAAAVASMLGGLVERSVSVSMPGGLLKVVWRDDGEVLLTGTAEV
                     VYEGVLRAGMEGESTEKR"
     gene            complement(2672..4429)
                     /gene="dacB"
                     /locus_tag="AC159P_08830"
     CDS             complement(2672..4429)
                     /gene="dacB"
                     /locus_tag="AC159P_08830"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine endopeptidase"
                     /protein_id="HIR3695596.1"
                     /translation="MPSRKNHAHARPTPFISRFLIIALLACATPSQPLAAQSRQRDRR
                     VNTQRPAPTPTPTTKQTPTPTPLAVPTPAPSDTPLDKDTAPAPQPSPTPTPAARQTAP
                     APQTLEELRARIREITSRPAFASSQLALKIVSLETGRVIYEENAHKWMQPASNMKLYT
                     VAAALDRFGPDYRFVTSVYAPARPDRAGLIKGDLTVYGRGDPTYATRFNPEDEEKNYY
                     RAIDELAARVVAAGVRKIEGDLVGDESYFAGIALAPGWEWDDLQWWYGAEVSALTVND
                     NSVDLAVKPGARVGDPCFITTGPPTSLVTVIDRTRTSARGTTRELVVHRPLGQNSIEV
                     SGTMPIDDRGFTASVAVSRPAMMFAAMLRASLERHGVVVTGKTRTIDARGRADSATPL
                     EVSALVELATRQSPPLSIIAAQTMKPSQNLYTELLLRALGKREATDPKKTSEESGVAA
                     VRSLLTKAGIDAGRLLIIDGSGLSRGNLVTAEATLQLLVYMNGHPHAHFFRDALPVAG
                     VDGTLRNRMRNTAATGNVRAKTGTLGSATSLSGYLFTAAGERLAFSLMINNPPRDTDP
                     RAGFTDAVAVLLASFTGRS"
     gene            5049..>8549
                     /locus_tag="AC159P_08835"
     CDS             5049..>8549
                     /locus_tag="AC159P_08835"
                     /inference="COORDINATES: protein motif:HMM:NF051698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR3695597.1"
                     /translation="MTGVVTDPAGAVISGATVTLSDPKTSLERTVTTDENGVYRFAQM
                     PPGQGYKLTFSGAGFQTLELTNVALGVGTVETHNVQLSAGAVAETITVVDQGGATLNT
                     TDASIGNVIEERRLKDLPIQIRSSPASLMGLQPGVIGNNVGTGATNRVGSVTGSRADQ
                     GNITVDGIDANDQSTGQAFATTGNAPIDAIQEFRTVSTNPGAAEGRSSGGQIQLITKS
                     GTNEFHGSLREFNRTALTSANSFFNNKNGVKRPQLTRNQFGGSIGGPVYLPRFGEGGD
                     RIWSGKDKLFFFFDYEGRRDAQGVSYLRTVPLAHVRAGGLAYLNSAAGCPTNARLTTR
                     PECITILTPAQVAALDPQGVGANQALLGFINERYPLPNDLTAGNGINTGGFRFNAPSL
                     RADNNYTARVDGNVTDRQKVFGRVSKISALQTDTVNSVAAQFPGDPETSQIVTGDYAV
                     AVGHTWVATSNLVNQATVGITKSILDFPNNFHPSSPQSFTFGLVSNPFAGFNTQAREV
                     PVWTYRDDVTWNKGSHGMQFGASVKTINQRSALVSDFNFPSVGLGGDLLSFGTGAVGT
                     ALRPANIGTGAARRDNYDAAFAFVLGVIPSVATRFNYDATGNALPLATGRNRDYRYEE
                     YEFYAQDSWRVRNDLTITFGARWHVYPAPYEVNGIQSIQNLDFETLFNRRVANGLAGI
                     AGAAAEPFLVYDLGGKANDARPFYDTDWNNFGPRLSFAYNPSFKDGLLGRLFGDRKTV
                     LRGGGTVTYDRPGGGITFLQDQSTYIFDTVVTTNFPVPSANARTALLNHPRFTNINTV
                     PILNVAPTVTTPFTPNVEDGVGVGTASQQTNYAVDPNFKIPYSLQYSFGFQREVPGNF
                     ILEVSYVGRQGRKLFTIADAAQIVDFKDPASGQFMLAAYNNLQAQIEAGGAITPQPWF
                     ENQILPEAQRNYGVTSCSQFGLGANCTELVANFLHSLVETGGSADLTQQLYSFALLRP
                     NVGLSSQFGTNGFVTNKGSSSYNGMLVSLRKRFSQGLQFDLNYTWSHSIDNQSTVANV
                     TTSGGLICDATNLRVCRGNSDFDIRHLFNANGLWEIPVGRGKFFAGEAPGWLDAIIGG
                     WQVSGIFAARSGLPFSLAAVAWPRTFIFDAANGVPPVISGNAAALRASIHDAPDGTIQ
                     FFADPEAARAATRYPRHGEAG"
BASE COUNT         1386 a         2831 c         2862 g         1470 t
ORIGIN      
        1 gacggcggcg agttggacgg ggttgtcggg ttggtcgtag gtttccgtcg gcgccccgag
       61 gtggacggcg agcgcgagca tcccagcgtg cttgcccgag cagttgttgt gcagcgcgct
      121 cggctgctcg ccgcgctcgc gcatctcttc cgccgtctgt ttgtcgaaag gctcgtggac
      181 gccgcacctc agagcgtcgg cgccgaggcc gatcttgcgg agcatctcgg cgaccgtctc
      241 ggcgtggacg ggttggccgc tgtgcgagcc gcaggcgatg gcgatctccc ggtccgtgaa
      301 ctgaaagcgt tcggcggcgc cggagacgag gagcgggatc gtctggtgcg gcttggccga
      361 cgagcgcagg aaggtgacgg tgtgcggcgt gccgaggtgc gccacgaccg cgccctcgcc
      421 gtcaaccgcg acgatgtgcc cgcggtggcg cgactccgtg atggcgccgc ggcggacttc
      481 gacgagcggc gcggcctcgg gcacttcgtt cttaggatta tcgctctcca tcgttcacac
      541 agattctcgc caccagcgcg gaagccgacg cgctcccgcg aggccgtggc agtcggtagc
      601 aacttgattt cataaggttg ggatgtcgtc gcatcttaac cgctttacgg cacaacggca
      661 aaggccgccg tacgcgtgta cggcggcctt tgtttcgtgc ggcgcgtcga aagcgcctcg
      721 ctatttgacg gtctgacttg ctttccccgt cgtgttgacg ggctgcgtcg ctttcccctt
      781 cgcgcggggt ctcgtgcggg tcagcaccgt gagtttgacc gggcggcgcc cgaagcgcat
      841 ggcttctcgc gtgctcggca tccagatgtc gatcttacga ccgcggacgg cgccgcccgt
      901 gtcggcgacc gtgtactcgc cgctgtaggg gccggcttcg aggcggacgc gcgtccctat
      961 gggaaggacg cggcggtctg ccgcgatgag gccccgcgcg acgggcttgc cgctcgccgt
     1021 gcgcccgcgc aggctgtagg ccgtggccgt gaaggacatc ggcgggcctg ccgcggtggt
     1081 cgtgttgtca acgggcgcct cggccttctt cgcctctcct atcggcgagg ccgcggcgcc
     1141 ttccccgact gtgttgctcg gcgtcagggg gtcgtttttg gcggaagcgt ccgccggggt
     1201 tgctgtctca ccgcctgtgt tcgtttgtgt tgccgtggcg gagacgcgtt tgtcctgcgt
     1261 ctgagattcc ggcgaggccg ttgcgagggt ctctgcgaca gaaggttgtg cgtaaagtat
     1321 caaagaagct gagagcagtg cgagcatcgc cgcactcccc cccagtaggg gttttgccat
     1381 tcttcggttc tcctcccgat taagtcttcc ggcggcgcgt gagtgtggtg ttgacttact
     1441 ctctgaccct tgctcaacct ccggctctag gagttaatga tcagtcagcc ggagtggggg
     1501 ctttcgcatc cttgatgcgg agcgccggaa gaacgcctaa aaagaaagac aagcgagctt
     1561 gaaccctcgc ttgtcacggg agaaaggcta cacaagtttg gcgaaagtgt ctaggcgcgg
     1621 ggcgggcgat agaggccgcg aaaacgtaag tgcggaaata gcacggctta gccggtcgat
     1681 ttttttcgat gattttatcg cttttccgta ctctcgccct ccattcccgc cctcaaaacc
     1741 ccttcgtaga cgacctccgc cgtgcccgtc aagaggactt cgccgtcgtc gcgccagacg
     1801 actttgagca gcccgccggg catcgagacg ctgacgcttc tctcgaccag gccgcccaac
     1861 atcgaggcga ccgccgccgc gcacgcgccc gtgccggaag agagcgtctc gccgacgccg
     1921 cgctcccaga cgcgcgcctc tatgttgcgc cggtcggcga cgcgtatgaa ctcgacgttg
     1981 gtgcgttcgg ggaagtccgc gtgcgactcg atgagacgac cgagccgccg ccagtccgtg
     2041 cgttcgaggt cttccgcgaa gaggcagcag ttcgggttgc acatctgcat ggcggtgacg
     2101 cggacggtct cgccgccggg gagcgcgagc ggctggtcgc gcacctccgc gagcggctgc
     2161 ggcgtccgca tcgggatcga ggcggagtcg aacaggggcc ttccgatctc tgcctcgaag
     2221 cggaaggcgc cctcgcccgt ccgctcgcgc agacggtagc gcttgacgcc tgcgcgcgta
     2281 cggaagcgca gctcctcgtc cgtccacagc ccgcgataat gcagataggc cgcggcgcag
     2341 cgcgagccgt tgccggacat cgccgcctcg ccgccgtccg ggttgaagat gcggagcgag
     2401 aagtccgcgt ccgcgcccgc ggccagacgc ccgacgatgg cgatgccgtc gccgcccgcg
     2461 ccgaagtggc ggtcgcagac gcgccgcgcg aactcgcccg gcgaagcgac gcccgccagc
     2521 tccgcctcct cgaagaccag atagtcgttg ccgtagccgt ggaatttgat gaatcgcata
     2581 acgtcagtga tcggaggtcg gaggtcggag gtcagtcaag atggagccag tctttcactg
     2641 ccccctaacc tccgacctct gacctccgtg gttatgatcg ccccgtgaag gacgcgagca
     2701 ggacggcgac ggcgtcggtg aagccggcgc gggggtccgt gtcgcgcggc gggttgttga
     2761 tcatgagcga gaaggcgagg cgctcgcccg ccgccgtgaa gaggtagccg gagagcgagg
     2821 tggcgctgcc gagcgtgccc gtcttcgcgc ggacgttgcc cgtcgcggcg gtgttgcgca
     2881 tgcggttgcg cagagtgccg tccacgcccg cgacgggcag cgcgtcgcgg aagaagtgtg
     2941 cgtgggggtg gccgttcatg tagacgagga gttgcagcgt cgcctccgcc gtcacgaggt
     3001 tgccgcgcga gaggccggag ccgtcgatga taaggagccg ccccgcgtcg atccccgcct
     3061 tcgtgagcag agacctgacg gcggcgacgc ccgactcctc gctcgtcttc ttcgggtcgg
     3121 tcgcctcccg tttgccgagc gcgcgcagca gcagctcggt gtagaggttc tggctcggct
     3181 tcatcgtctg cgcggcgatg atggagagcg gcggcgactg ccgcgtcgcc agctcgacga
     3241 gcgcggagac ttcgagcggc gtcgccgagt cggcgcgacc gcgcgcgtcg atcgtccgcg
     3301 tctttccggt gacgacgacg ccgtggcgtt cgagcgaggc gcgcagcatc gcggcgaaca
     3361 tcatcgcggg ccgcgagacg gcgacgctcg ccgtgaagcc gcggtcgtcg atgggcatcg
     3421 tgccggagac ctcgatgctg ttctgcccga gcgggcggtg gacgaccagc tcgcgcgtcg
     3481 tgccgcgcgc gctcgtccgc gtgcggtcga tgacggtgac gagcgaagtg ggcggccccg
     3541 tggtgatgaa gcacgggtcg ccgacgcgcg cgccgggctt gacggcgaga tcgaccgagt
     3601 tgtcgttgac ggtgagcgcg ctcacctccg cgccgtacca ccattgcagg tcgtcccact
     3661 cccagccggg cgcgagcgcg atgcccgcga agtagctctc gtcgccgacc agatcgccct
     3721 ctatcttccg caccccggcg gcgacgacgc gcgccgccag ctcgtcgatg gcgcggtagt
     3781 agttcttctc ctcgtcctcg gggttgaagc gcgtggcgta ggtcgggtcg ccgcgcccgt
     3841 agacggtgag gtcgcctttg attagccctg cgcggtcggg gcgcgcgggc gcgtagaccg
     3901 aggtgacgaa gcggtagtcg gggccgaagc ggtcgagcgc ggcggccacc gtgtagagct
     3961 tcatgttcga ggcgggctgc atccacttgt gcgcgttctc ctcgtagatg acgcgcccgg
     4021 tttcgagcga gacgatcttc agcgcgagct gcgaagaggc gaacgcgggc cgcgacgtga
     4081 tctcgcggat gcgcgcgcgc agctcttcga gcgtctgcgg cgcgggcgcc gtctggcgcg
     4141 cggcgggcgt cggcgtcggc gaaggctgtg gcgcgggcgc cgtgtccttg tcgagcggcg
     4201 tgtcgctcgg cgccggcgtc ggaaccgcga gaggggtcgg cgtcggcgtc tgcttcgtcg
     4261 tgggggtcgg cgtcggcgcg gggcgctgcg tgttgacgcg gcggtcgcgc tgccggctct
     4321 gcgcggcgag cggctgcgaa ggggtcgcgc acgcgagcag cgcgatgatt aagaaacggg
     4381 agatgaaggg cgtcggtcgt gcgtgtgcgt ggttcttcct tgaaggcata agcgacgaat
     4441 gatgtctccg gctgatcgtc cttgcgcgcg ttagagtgtg aacgaaacgg agccattcgg
     4501 caaatgcttc tgcgaggtct gcggggcgcg cgcgggttcc gcgcggcgga cttaatccac
     4561 gccaatcgac aggcattcgc gcgccccgtc cagccctttg gacgggcctt tcgggcgcgt
     4621 cttcaaaact tcgtatcata tctccaaaac ccgcaagatg attaggaggc gccgcggcgg
     4681 tcgcgccggc ggcctccgcc gaggggttgg atttagcccg ccgcacgctt ccgggcacaa
     4741 gccttgcacc gtccacagag cagcagttca ggggacggaa cttaccactt cttccccgct
     4801 cgacaatcca ttttccggta cgcgcgcggg aaggccgtga tgaaaagatg aatcttcccc
     4861 gccgtgaccg cctcggcaat tcggcggagg ctttagcacc ccggactgcg gtcgccagac
     4921 actttgcctc aaccagaaca aggagagaga agagtatgca taaagcgaca gctatcttag
     4981 ccacgtcgtt gctcctgctg ctgtgcgtag caggagtttt cgcccaagcg acttcttcca
     5041 cgtccgcggt gacgggagta gttacagacc ccgccggcgc cgtcatcagc ggcgcgaccg
     5101 tcacactatc cgaccccaag acctcgctgg agcggacggt gacgaccgat gaaaatgggg
     5161 tttatcggtt cgcccagatg ccgcccggtc agggctacaa actgaccttt agcggggcgg
     5221 gtttccagac tttggaactg accaacgtcg cgctcggggt gggcaccgta gagactcata
     5281 acgtgcagtt gtccgccggc gccgtcgccg aaacgataac tgtcgtggat cagggcgggg
     5341 ccactctgaa cacgaccgac gccagtatcg gcaacgtcat cgaggagcgg cggctgaagg
     5401 acctcccgat tcagatccgc agctcgcccg cctcgctgat gggcctacag cccggcgtga
     5461 tcggaaacaa cgtggggacc ggggccacca accgcgtcgg ctcggtgacg ggttcgcgcg
     5521 ccgaccaggg taacatcacg gtcgatggga tcgacgccaa cgaccagtcg accgggcagg
     5581 cgttcgcgac gacgggcaac gcacccatcg acgcgattca ggaattccga accgtctcga
     5641 ccaaccccgg cgcggcggaa ggtcgcagca gcggcggcca gattcagctc atcaccaaga
     5701 gcggcaccaa cgagttccac ggctcgctgc gcgaattcaa ccgcaccgcg ctcacgtcgg
     5761 cgaactcctt tttcaataac aagaatgggg ttaagcggcc ccagttgacc cgcaaccagt
     5821 tcggcggctc gatcggcggc cccgtctacc tgccgcgctt cggcgagggc ggcgaccgca
     5881 tctggagcgg caaagacaag ctgttcttct tcttcgatta tgaagggcgg cgcgacgcgc
     5941 agggcgtcag ctacttgcgc accgttcccc tcgcgcacgt ccgtgcaggc ggcctggcgt
     6001 acctgaacag cgccgccggt tgtccgacca acgcccgcct caccacgcgg cccgagtgca
     6061 tcacgattct cacgccggcg caggtggcgg ccctcgaccc gcagggggtc ggcgccaatc
     6121 aggccctcct gggctttatc aacgagcgat accctctgcc caacgatctg accgccggca
     6181 acgggatcaa caccggcgga ttccgtttca acgcgccctc tctgagagcg gacaataact
     6241 atacggcgcg cgtggacggg aacgtgacgg acaggcagaa ggtgttcggg cgcgtcagta
     6301 agatcagcgc cctgcaaacc gacacggtga attcggtggc cgcgcagttc cccggagacc
     6361 cggagacctc gcagatcgtg acgggcgact atgcggtggc cgtcggccac acctgggtcg
     6421 ccacctccaa ccttgtcaat caggcgacgg tcgggatcac gaaatccatt ctggatttcc
     6481 cgaacaactt ccacccctcg tccccccaat ccttcacttt cggtctggtc tcgaacccct
     6541 tcgccggctt caacacccag gcccgcgagg tgcccgtgtg gacgtaccgc gacgacgtga
     6601 cgtggaacaa aggctcgcac ggcatgcaat tcggcgcctc ggtcaagacg atcaaccagc
     6661 ggtcggccct cgtcagcgac ttcaacttcc cgagcgtcgg cctcggcggc gacctgctga
     6721 gcttcggcac cggtgccgtc ggcacagcgc tgcggcccgc caacatcgga accggcgccg
     6781 cgcgcagaga caattatgac gcggcgttcg ccttcgtcct gggggtgatc ccgtcggtcg
     6841 cgaccagatt caattacgac gcgacgggca acgccttacc gctagccacc ggcaggaacc
     6901 gcgactaccg ctacgaagaa tacgaattct acgcgcagga tagctggagg gtccgcaacg
     6961 acctgacgat caccttcggg gcgcgctggc acgtttaccc cgcgccgtac gaggtcaacg
     7021 gaatccagtc gatccagaac ctggacttcg agaccttgtt caaccggcgg gtggcgaacg
     7081 ggctggccgg catcgccggc gctgccgccg agcctttcct ggtctacgac ctgggcggca
     7141 aggcgaacga cgcccgcccc ttctacgaca cggattggaa caacttcggc ccgcggttga
     7201 gtttcgctta caacccctcg ttcaaggacg ggctgctggg cagactcttc ggcgaccgga
     7261 agacggtgct ccgcggtggc gggacggtca cctacgaccg ccccggaggc ggcatcacct
     7321 tcctccagga ccagagcacc tacatcttcg acaccgtcgt gaccaccaac ttccccgtcc
     7381 cgagcgcgaa cgcgcgcacg gccctgctga accacccgcg gttcaccaac atcaacaccg
     7441 tgccgatcct gaacgtcgcg ccgacggtca ccaccccgtt cacccctaac gtcgaggacg
     7501 gggtcggggt cgggactgct tcccagcaaa ccaactacgc cgtagacccg aacttcaaga
     7561 taccctactc gctccagtat agcttcggtt tccagcgcga ggtgccgggc aatttcatcc
     7621 tcgaagtgtc gtacgtcggg cggcaggggc ggaagctctt cacgatcgcg gacgccgcgc
     7681 agatcgtcga tttcaaagac ccggcttcgg gccagttcat gctcgccgcc tacaacaacc
     7741 tgcaagcgca gatcgaggcg ggcggggcca tcacgccgca gccgtggttc gagaatcaga
     7801 tcctcccgga ggcccaaagg aactacggcg tcaccagttg cagccagttc gggctgggcg
     7861 ccaactgcac ggaactggtg gccaacttct tgcactcgct ggtcgagacc ggcggctccg
     7921 ccgacctcac ccagcagctc tactccttcg cgctgctcag gccgaacgtg ggcctcagct
     7981 cgcagttcgg gaccaacggc ttcgtcacga acaaggggtc gtcgagctac aacgggatgc
     8041 tggtcagcct gcgcaagcgc ttctcgcagg ggcttcagtt cgacctcaac tacacctggt
     8101 cgcactcgat cgacaaccaa tcgacggtcg cgaacgtcac cacgtccggc ggcctgatct
     8161 gcgacgcgac caacctccgc gtctgccgcg gtaactccga cttcgacatc cgccacctct
     8221 tcaacgcgaa cggtctctgg gagattcccg tggggcgcgg gaagttcttc gccggggagg
     8281 cgcccggctg gctggatgcc atcatcggcg gctggcaggt gagcggcatt ttcgccgccc
     8341 ggagcggact gcctttcagc ctggccgcgg tcgcctggcc gcggactttc atcttcgacg
     8401 ctgccaacgg cgtgcccccg gtgatctcgg gtaacgcggc tgcactgcgg gcctcgatcc
     8461 acgacgcgcc ggacgggacg atccagttct tcgccgaccc ggaggcggcg cgcgcggcga
     8521 cgcgttaccc ccgccacggc gaagcgggc
//
LOCUS       DBNGOH010000445         3242 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1351355, whole genome shotgun sequence.
ACCESSION   DBNGOH010000445 DBNGOH010000000
VERSION     DBNGOH010000445.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3242)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3242)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3242
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1351355"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..493
                     /locus_tag="AC159P_08840"
     CDS             <1..493
                     /locus_tag="AC159P_08840"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3695598.1"
                     /translation="LAPRAQEVNFRVVAEGRPMPLHPVIRDEVYRIGREALVNAFRHA
                     EAGGVEVEVEYAPKHLRVLVRDDGRGIDAEVLRSGRDGHWGLSGMRERAERIGARLKV
                     RSRAASGTEVELNVPGHVAYRVQPAARTHGWLAALRRRKATDAATQAGAGADREAGSG
                     GDE"
     gene            567..1241
                     /locus_tag="AC159P_08845"
     CDS             567..1241
                     /locus_tag="AC159P_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012408641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HIR3695599.1"
                     /translation="MKLISINVARPRLVVWKGEPVSTGIFKEPVEGRVMLRTLNLDGD
                     RQADLAVHGGPTKAVYAYPSEHYEFWRAELPEMNLPYGMFGENFTTEGLDEESVRIGD
                     RFRVGEAEVTVTEPRMPCYKLGIKFGRADILKRFLRSGRTGFYFSVSREGEVGAGDAI
                     ERLSRDEHNVTVADITRLYASDRDDLHTMRRALAVAALPAGWKDYFRERLEKRTGAPG
                     DADPEA"
     gene            1371..>3242
                     /locus_tag="AC159P_08850"
     CDS             1371..>3242
                     /locus_tag="AC159P_08850"
                     /inference="COORDINATES: protein motif:HMM:NF012801.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR3695600.1"
                     /translation="MSARVASKTLVLMLALASSVCGAKALAAQGGVARTPRVGGVVTD
                     TAGAPVGGAQVTLSAGAAHDSNTGASRDRRALTGEDGRFVFEGVRATEGRLAVSAEGF
                     ARRELSWRVSEGNVAEIEVVLAPAPLSERVTVTATRTRTKLGETAASVVVLTPEALAT
                     TAAVTLDDALRQVPGFQLFRRTGSRAANPTAQGVSLRGVGASGASRALVLADGVPLND
                     PFGGWVYWGRVPRQSVGSVEVLRGGASDLYGSSALGGVVNVLTRGVEDRPALAFEASY
                     GTQRTLDGSLFVGGRKRGWGASLAAESFHTGGYHLVKREERGAADTPAASRYKALNVS
                     VEREAGAGVRVFARGSYFGEARDNGTRLQRNRTHVRQAVVGADLARERIGAFTFRAHF
                     GTQVFDQSFTAVASDRESETLTRNQRVPAQAAGFLAQWSRGFGARHALVAGFEAREVR
                     GASDELVYVAGRASSLVGAGGRERAAGVFVEDIVRLSAKLTLTAGGRFDRWRNHGAHS
                     TTAPLSGPATTNAFPDRAESAFSPRASLLYNANARVSLHASGYRAFRVGNVLTLANEN
                     LRAERLAGGEAGAGFVSRGKRFAARATFFRLELTRPVANVTLSETPQLITRQRQSLGR
                     "
BASE COUNT          479 a         1123 c         1200 g          440 t
ORIGIN      
        1 gctggcgccg cgcgcgcagg aggtcaactt ccgcgtcgtc gccgaaggcc gaccgatgcc
       61 gctgcacccg gtcatccgcg acgaggtcta ccgcatcggg cgcgaggcgc tggtcaacgc
      121 cttccgccac gccgaggccg ggggcgtcga ggtcgaggtc gaatacgcgc cgaagcactt
      181 gcgcgtgctc gtccgcgacg acggtcgcgg catcgacgcc gaagtgctgc gctccgggcg
      241 cgacgggcac tgggggctgt cgggcatgcg cgagcgcgcg gagaggatcg gcgcgcggct
      301 caaagtccgc agccgcgccg cgagcgggac ggaggtcgag ctgaacgtgc ccggccacgt
      361 cgcctaccgg gtgcaacccg ccgcacgcac gcacggctgg ctcgccgccc tgcgccgtcg
      421 caaagcaacc gacgcagcca cgcaggccgg ggccggcgcg gatagagagg ccgggagcgg
      481 cggggacgaa tgagcggcag agatgaatga gcggcggggc gcgaggtctt acgtcgcgcc
      541 tcgccgggcg ggtgtatgct atggagatga agctgatttc catcaacgtc gcgcggccac
      601 ggctggtggt gtggaagggc gagccggtct cgacgggcat cttcaaagag ccggtcgagg
      661 ggcgtgtgat gctccgcacg ctcaacctcg acggcgaccg gcaggccgac ctcgccgtcc
      721 acggcgggcc gaccaaggcg gtctacgcct acccgtccga gcactacgaa ttctggcgcg
      781 cagagctgcc cgagatgaac cttccctacg ggatgttcgg cgagaacttc acgaccgaag
      841 gtctcgacga agaaagcgtc cgcatcggcg accgcttccg cgtcggcgag gccgaggtga
      901 cggtgacgga gccgcgcatg ccctgctaca agctcggcat caagttcggg cgcgccgaca
      961 tcctcaaacg ttttctgcgg agcgggcgca cgggcttcta cttctccgtc tcgcgcgagg
     1021 gcgaggtcgg cgcgggcgac gccatcgagc ggctcagtcg tgacgagcac aacgtcacgg
     1081 tcgcggacat cacgcgtctc tacgcgagcg accgcgacga cctccacacg atgcggcgcg
     1141 cgctggcggt cgcggcgctc cccgcgggct ggaaggacta cttccgcgaa cgcctggaga
     1201 agcgaacggg agcgccgggc gacgccgatc cggaagcgtg acgcggggcg ggccgcacgt
     1261 cgcggaggtg aagccgggcg acgacatcgt cttcgacggc agctccgcgc tcggcaggaa
     1321 cggcgtgctc gtgctctcct cagatactgt cggagatacc ttcgaatatg atgtcagcgc
     1381 gggtggcctc gaagactctg gtgctgatgc tcgcgctcgc ctcttcagtc tgcggcgcga
     1441 aagcgctcgc cgcgcaaggc ggcgtcgcac ggacgccgcg cgtcggcggc gtcgtgaccg
     1501 acacggcggg cgctccggtc ggcggcgcgc aagtcactct gagcgcgggc gcggctcacg
     1561 actcgaacac gggcgcgtcc cgcgaccgtc gcgcgttgac gggtgaggac ggtcgcttcg
     1621 tcttcgaggg ggtgcgcgcg accgagggca ggctcgcggt ctccgccgaa ggcttcgcgc
     1681 ggcgggagct gagttggaga gtgtcggagg ggaacgtcgc ggaaatcgag gtggtgctcg
     1741 cgcccgcgcc cttgagcgag cgcgtgacgg tgacggcgac gcggacacgg acgaagctcg
     1801 gcgagacggc ggcgagcgtc gtcgtgctca cgcccgaagc tctcgccacc accgccgccg
     1861 tcacgctcga cgacgcgctg cgacaggtgc ccggcttcca actgttccgc cgcacgggca
     1921 gccgcgccgc caacccgacc gcgcagggcg tctctctgcg cggcgtcggc gcgagcgggg
     1981 ccagccgcgc cctcgtgctc gcagacggcg tgccgctgaa cgaccccttc ggcgggtggg
     2041 tttactgggg ccgcgtgccg cgccagtccg tcggcagcgt cgaagtgctg cgcggcggcg
     2101 cgtccgatct ctacggcagc tcggcgctcg gcggcgtcgt caacgtcctc accagaggcg
     2161 tcgaagaccg accggcgctc gccttcgagg cttcttacgg gacacagcgg acgctcgacg
     2221 gctcgctttt cgtcggcggt cgcaagcgag gctggggcgc gagtctcgcc gcggagtctt
     2281 tccacacggg cggctatcac ctcgtcaaac gggaagagcg cggcgcggcg gacacgcctg
     2341 ccgcttcgag atacaaggcg ctcaacgtct cggtcgagcg cgaggcgggc gcgggcgttc
     2401 gcgtcttcgc gcgcggctcc tacttcggcg aggcgcgcga caacggcacg cgtctccaac
     2461 gcaaccgcac gcacgtcagg caggccgtcg tcggcgccga cctcgcacgc gagcgcatcg
     2521 gcgcctttac cttccgcgcg cacttcggca cgcaggtctt cgaccagagt ttcaccgccg
     2581 tggcgagcga ccgcgagagc gaaacgctca cgcgcaacca gcgcgtgccc gcgcaggccg
     2641 cggggtttct cgcgcaatgg tcgaggggct tcggcgcgag gcacgcgctc gtcgcgggct
     2701 tcgaggcgcg cgaggtgcgc ggcgcgagcg acgagttggt ctacgtggcg ggccgcgctt
     2761 cgagtctggt cggcgcgggc ggcagggagc gggcggcggg cgtcttcgtc gaagacatcg
     2821 tgcggctctc cgcgaagctg acgctgacgg cgggcgggcg cttcgaccgc tggcgcaacc
     2881 acggcgcaca cagcacgacc gcgcccctct ccggccccgc gacgacgaac gccttcccag
     2941 accgcgccga gtccgccttc agcccgcgcg cgtcgctgct ctacaacgcg aacgcgcgcg
     3001 tctctctcca cgcctccggc tatcgcgcct tccgcgtcgg gaacgtgctg acgctggcga
     3061 acgagaatct gcgcgccgag aggctcgcgg gcggggaggc gggggcaggc ttcgtctcgc
     3121 gcggcaagag gttcgccgcg cgcgccacct ttttccgtct ggagttgacg cgccccgtgg
     3181 ccaacgtgac gctgagcgag acgccgcagc tcatcacgag gcaaaggcag agcctcgggc
     3241 gg
//
LOCUS       DBNGOH010000446         1598 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2633528, whole genome shotgun sequence.
ACCESSION   DBNGOH010000446 DBNGOH010000000
VERSION     DBNGOH010000446.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1598)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1598)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1598
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2633528"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            225..1430
                     /gene="ribB"
                     /locus_tag="AC159P_08855"
     CDS             225..1430
                     /gene="ribB"
                     /locus_tag="AC159P_08855"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020675844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HIR3695601.1"
                     /translation="MPFASIEEAARDIGEGRLVIIVDDEDRENEGDLACAAEKITPEI
                     INFMATHARGLVCMPLTEERCDELHLTMQVADNTSYLGTAFTVSIEARKGVTTGISAA
                     DRATTILTAVDPQTRPQDLARPGHVFPLRAKKGGVLVRTGQTEAAVDMARIAGLYPAG
                     VICEIMNEDGTMARLPQLEEFAARHGLKMITVADLVRYRMRKEVMVRRAVESELPTVY
                     GRFRAVAFENVINGDLHLAMVMGDVGRAGDEPVLVRVHTENVTCDMFGSLVDDTGFQL
                     HAALERIAAAGRGVVLYLRQRENNLDLVRQLKTYQLMHERGLSKEEASLETGYGAERD
                     YGVGAQILHELGLRRILLLSNHPPKVSALEGFELEIVGNVPLGAPAREEKTKGAFPVG
                     EAKPLAPES"
BASE COUNT          278 a          569 c          502 g          249 t
ORIGIN      
        1 ggggcaggga caagccctgc ccctacagtc atcacgcgcg ttgtacaact tgccgaactc
       61 gccctaaatc cccgcggctg attccggcgc cgaacgtcgc cgcgcttttt cactctgctt
      121 ccgcgccccg cccgttttta ccagtcgcca aacgcccgcc tttcgggtaa catagctcgc
      181 gactttccca tctcccgaac agccgtcagg cgagtgttaa gagcatgcct tttgcttcca
      241 tcgaagaggc cgcgcgcgac atcggcgagg gccgactggt catcatcgtg gacgacgaag
      301 accgcgagaa cgagggcgat ctggcctgcg cggcggagaa gatcacgccc gaaatcatca
      361 acttcatggc gacgcacgcg cgcggcctcg tctgcatgcc cttgaccgag gagcgctgcg
      421 acgagctgca cctgacgatg caggtcgccg acaacacctc gtatctcggc accgccttca
      481 ccgtctccat cgaggcgcgc aagggcgtca ccaccggcat ctccgccgcc gaccgcgcga
      541 cgaccatcct cacggcggtc gatccgcaga cgcgcccgca agacctcgcg cgtcccggcc
      601 acgtcttccc cttgcgagcg aagaagggcg gcgtgctcgt ccgcaccggc cagaccgagg
      661 ccgccgtgga catggcgcgc atcgcgggcc tgtatcctgc gggcgtcatc tgcgagatca
      721 tgaacgagga cgggacgatg gcgcggctgc cgcagctcga agagttcgcc gcgcgccacg
      781 gcctgaagat gattacggtg gccgacctcg tccgctaccg gatgcgcaaa gaggtgatgg
      841 tgcgccgcgc ggtcgagtcg gagctgccta cggtctacgg gcgcttccgc gccgtcgcct
      901 tcgagaacgt catcaacggc gacctccatc tggcgatggt gatgggcgac gtgggccgtg
      961 cgggcgacga gcctgtgctc gtccgcgtcc acaccgagaa cgtgacgtgc gacatgttcg
     1021 gctcgctcgt ggacgacacg ggcttccagc tccacgccgc gctcgaacgc atcgccgccg
     1081 cgggccgggg cgtcgtcctc tacctccggc agcgggagaa caacctcgac ctcgtgcggc
     1141 agctcaagac ctaccagctc atgcacgagc gcggcctgtc gaaagaggag gcgagcctcg
     1201 agacgggcta cggcgccgag cgcgactacg gcgtcggcgc gcagatactc cacgagctgg
     1261 gcttgcggcg catcctgctg ctctcgaacc acccgccgaa ggtctcggcg ctcgaaggct
     1321 tcgagctgga gatcgtcggc aacgtgccgc tcggcgcccc cgcgcgcgag gagaagacga
     1381 agggcgcttt ccccgtcggc gaggcgaaac cgctcgcgcc agagtcctga acacggtttt
     1441 caagttctcc cttctaaccc aacacagacc gccgtcgttt cccccttcag cggacggctc
     1501 gttgaagaaa gccttatgcc tgctgacgag cacgacgaac gtccctccag cccgtcgccg
     1561 gaagacgcgg gcccggtcaa gttcgaggag ccgcccgg
//
LOCUS       DBNGOH010000447         2038 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4649705, whole genome shotgun sequence.
ACCESSION   DBNGOH010000447 DBNGOH010000000
VERSION     DBNGOH010000447.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2038)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4649705"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..394
                     /locus_tag="AC159P_08860"
     CDS             <1..394
                     /locus_tag="AC159P_08860"
                     /inference="COORDINATES: protein motif:HMM:NF024970.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR3695602.1"
                     /translation="GDDTELIAASTVEQIARRLDFQPEAINQIKTALVEACINAAEHS
                     LSPDRKIYQRFRLESDKLTVTVSSRGRTIPPPTAEQSNGHDTNTTERGVKGRRGWGLK
                     LIRTLMDEVEFEPVDDGTRLRMTKYLRK"
     gene            411..803
                     /locus_tag="AC159P_08865"
     CDS             411..803
                     /locus_tag="AC159P_08865"
                     /inference="COORDINATES: protein motif:HMM:NF013868.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HIR3695603.1"
                     /translation="MSDVSAFLHGPVPTGSGDIAVVYAGDYVNKLSGQRLERECRLRL
                     EAGCRALVINFKGTELVNSIGVSILLGVIDIAEERSAPVVFTNVNRHTYKLFELLGLT
                     RHVSMAESEEAALATLAGFFAESIPPGH"
     gene            881..>2038
                     /locus_tag="AC159P_08870"
     CDS             881..>2038
                     /locus_tag="AC159P_08870"
                     /inference="COORDINATES: protein motif:HMM:NF024878.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HIR3695604.1"
                     /translation="MSDRLRKVLHAFSALADLGEELAETSDYEEMVASALHVVLGALG
                     ARRGAVAEYDPEAALLRFVAARGTGADDGLHFPPESFAPPAPADAPHANGHADAPLDT
                     SLVEELIGARVRHLPVELVSALVVRGELVGAILIGGKASDEPFTDEDRELVRALGRHV
                     GIGLHNHRLLREVERRAGENRKLYDDLRALYKDTVRAFAAAIDCKDKYTQGHSERVGK
                     YSEIIARELGWADEEVEGIAVAGYLHDIGKLVVDREIINAPYRIDAKQSSQLNRHPAT
                     GYEILSPINHPYADIPQAARYHHERVDGRGYPEGLTGDQIPLCAKIVTLADSFDAMTT
                     DRPYKRRRAFEDVVADFRRNTGRQFDPAVVAAFCRALLKEVRGETRPRQIIR"
BASE COUNT          375 a          767 c          627 g          269 t
ORIGIN      
        1 cggcgacgac acggagctga tcgccgcgag caccgtcgag cagatcgcgc gccgcctcga
       61 cttccaaccc gaagccatca accagatcaa gacggcgctc gtcgaggcgt gcatcaacgc
      121 cgccgagcac agcctcagcc ccgaccgcaa aatttatcaa cgtttccggc tggaaagtga
      181 taaattaacg gtaacagtgt cgagccgagg ccgcaccatc ccccccccga cggcggaaca
      241 gagcaacggg cacgacacca acaccacgga gcgcggcgtg aaggggcggc gcggctgggg
      301 tctgaaactc atccgcacgc tgatggacga ggtcgagttc gagccggtag acgacggcac
      361 ccgcctccgc atgacgaagt acctccgcaa gtaagaaggg aacctgcctg atgtccgatg
      421 tcagcgcctt tctgcacggg cctgtcccca caggctccgg cgacatcgcc gtggtctacg
      481 ccggcgacta cgtcaacaag ctgagcggcc agcgcctcga acgcgagtgc cggctccgcc
      541 tcgaagcggg ctgccgcgcc ctcgtcatca acttcaaggg cacggagctg gtgaacagca
      601 tcggcgtctc gatcctgctc ggcgtcatcg acatcgccga agagcggagc gcgcccgtcg
      661 tcttcacgaa cgtcaatcgc cacacgtaca agctgttcga gctgctcggc ctgacgcgtc
      721 acgtctcgat ggccgagagc gaagaggccg cgctcgccac gctcgccggc ttcttcgccg
      781 aatcgatccc acccggccat tgacgggcga aggctcccga cgccgccgac aaagccaccc
      841 cccccgacgt taggccgtca ccgacgagca ccgcataacc atgtccgacc gtttgcgcaa
      901 agtcctgcac gccttctcgg cgctcgccga cctcggcgag gagctggccg agacgagcga
      961 ctacgaggag atggtcgcct cggccctgca cgtcgtgctc ggcgcgctcg gcgcgcggcg
     1021 cggcgccgtc gccgagtacg acccggaagc ggcgctcctg agattcgtcg ccgcgcgcgg
     1081 cacgggcgcc gacgacggcc tccacttccc gccggagtcg ttcgccccgc ccgcgcccgc
     1141 cgacgccccg cacgcgaacg ggcacgccga cgcgccgctc gacacctcgc ttgtcgagga
     1201 actgatcggg gcgcgcgtcc gccacctgcc cgtcgaactc gtctcggcgc tcgtcgtccg
     1261 cggcgaactc gtcggcgcga tcctcatcgg cggcaaggcg agcgacgagc ctttcacgga
     1321 cgaagaccgc gaactcgtcc gcgcgctcgg gcgccacgtc ggcatcgggc tgcacaacca
     1381 ccgcctgctc cgcgaggtcg agcggcgcgc aggggagaac cgcaagctct acgacgacct
     1441 gcgtgcgctc tacaaagaca cggtgcgcgc cttcgccgcc gccatcgatt gcaaggacaa
     1501 gtacacgcag gggcactcgg agcgcgtcgg caagtacagc gagatcatcg cgcgcgagct
     1561 ggggtgggcg gacgaagagg tcgagggcat cgccgtcgcg ggctacctgc acgacatcgg
     1621 caagctcgtc gtggatcgcg agatcatcaa cgcgccttac aggatcgacg ccaagcagtc
     1681 ctcgcagctc aaccgccacc cggccaccgg ctacgaaatc ctctcgccca tcaaccaccc
     1741 ctacgcggac atcccgcagg ccgcgcgcta ccaccacgag cgcgtggacg ggcgcggcta
     1801 ccccgaaggg ctgaccggcg accagatacc cctgtgcgcc aagatcgtca cgctcgccga
     1861 ctcgttcgac gcgatgacca ccgaccgccc ctacaagcgc cgccgcgcct tcgaagatgt
     1921 ggtcgccgat ttccgccgca acacgggccg ccagttcgac cccgcggtcg tcgccgcctt
     1981 ctgccgcgcg cttctcaaag aagtccgcgg cgagacgcgc ccacgccaga tcatccgc
//
LOCUS       DBNGOH010000448         1867 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3368429, whole genome shotgun sequence.
ACCESSION   DBNGOH010000448 DBNGOH010000000
VERSION     DBNGOH010000448.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1867)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1867)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1867
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3368429"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..357)
                     /locus_tag="AC159P_08875"
     CDS             complement(<1..357)
                     /locus_tag="AC159P_08875"
                     /inference="COORDINATES: protein motif:HMM:NF014017.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HIR3695605.1"
                     /translation="MHTDDSRKVQVGCQGWNYEDWVTPAAAERAVFYPRGTRADRMLD
                     TYARAFDTVEVDSTFYAAPAESVLEGWARRTPAGFTFALKLPQEITHEHSLRGPRAAA
                     ALADFCARARRLGPKLA"
     gene            complement(375..1034)
                     /locus_tag="AC159P_08880"
     CDS             complement(375..1034)
                     /locus_tag="AC159P_08880"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3695606.1"
                     /translation="MGFDSPDGHSEARSGGQTYDRLAARYDRAMRPLERWGLGRLRAA
                     ALGHLPEGARLLEVGAGTGANFALYPRAARAVASEPSGEMLKVARDKRARAHENDGGA
                     RRISLVRARAEQLPFADGAFDAACATLVFCSVASPARGFAELRRVVRAGGTVVLLEHV
                     RPAGLLGYAFDALSLLTVALFEDHFNRRTADEARGAGLRVLSVEKHLLGAVQIIVCRV
                     E"
     gene            1386..>1867
                     /locus_tag="AC159P_08885"
     CDS             1386..>1867
                     /locus_tag="AC159P_08885"
                     /inference="COORDINATES: protein motif:HMM:NF025006.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HIR3695607.1"
                     /translation="MARAVKSMIALMALLLCASWPVAAQTTGSISGTVQDEKEGTLPN
                     ATVTVRNVQTSAARTTQTNDDGSYRFTNLPVGNYEVTVEAAGFAKYVQTGIELLLNQD
                     AVVNATMKAGAVQETVTVTENAALLNTTTAEVSTRFDSRRLSELPISPNRSIYLVALS
                     A"
BASE COUNT          333 a          640 c          612 g          282 t
ORIGIN      
        1 ggccagcttc gggccgagcc gccgggcgcg cgcgcagaag tccgccagcg ccgcggccgc
       61 gcgcgggccg cgcagggagt gttcgtgcgt gatctcctgc gggagcttga gcgcgaaggt
      121 gaagcccgcg ggcgtgcgcc gcgcccaccc ttcgagcact gactcggccg gcgcggcgta
      181 gaaagtcgag tcgacctcga cggtgtcgaa ggcgcgcgcg taagtgtcga gcatgcggtc
      241 ggcgcgcgtg ccgcgcgggt agaagaccgc gcgctcggcg gcggcgggcg tcacccagtc
      301 ttcgtagttc cagccctgac agccgacttg aaccttgcga gagtcgtcag tgtgcatcat
      361 ggcccccgcc gccatcactc gacgcggcat acgattatct ggacggcgcc gagcaggtgc
      421 ttctcgaccg agagaacgcg cagccccgcg ccgcgtgcct cgtcggcggt gcggcggttg
      481 aagtggtctt cgaagagcgc gaccgtgagc aggctgagcg cgtcgaaggc gtagccgagc
      541 agacccgccg ggcgcacgtg ttcgagcagc acgaccgtgc cgcccgcgcg caccacccgg
      601 cgcagctccg cgaagccgcg cgcgggcgag gcgaccgagc agaagacgag cgtggcgcag
      661 gccgcgtcga acgcgccgtc ggcgaagggc agttgctcgg cgcgcgcccg cacgaggctg
      721 atcctccgcg cgccgccgtc gttttcgtgt gctcgcgctc tcttgtcgcg cgccactttc
      781 agcatctcgc cgctcggctc gctcgccacc gcgcgcgccg cgcgcgggta gagcgcgaag
      841 ttcgcgcccg tgcccgcgcc gacttcgagc aggcgcgcgc cctcgggcag gtgcccgagc
      901 gccgccgcgc gcaggcgacc gaggccccag cgttcgagcg gtcgcatcgc gcggtcgtag
      961 cgcgcggcca gccggtcgta ggtctggccg cctgaacgcg cctccgagtg gccgtccggc
     1021 gaatcgaagc ccatcctgta aattcggttt tcttcgcgaa aatgcatgaa attatccaaa
     1081 aaaatggata actccgggcg cctcacatgt aaaagttgaa aaaaaacggt ggccgaaaaa
     1141 ggcataccgc ttgcgtccga ttaaaaccgc ctgccgcctt caaacgacca gcaatcattc
     1201 ttgacggtca cggcagcgcc tccgcaagta ttaatttaag cacatctctc atcagtgacg
     1261 agcgccaaag ctttcgtcgg tcatcgtgta agcacctcgc agtcgagttt tccggatcag
     1321 ccgtgcgggt cggtcggtca actttaacaa tccttaccca ccccgttcag gaggagaata
     1381 aaagtatggc aagagcagtg aagtccatga tcgcactcat ggcgctgttg ctgtgtgcgt
     1441 catggcccgt cgcggcccag acgaccggct ccatttcggg cactgtgcag gacgagaagg
     1501 agggcacgct ccccaacgcg accgtcaccg tccgtaacgt ccagaccagc gcggcgcgca
     1561 ccacgcagac gaacgacgac ggcagctacc gcttcacgaa cctgccggtc ggcaattacg
     1621 aggtgacggt cgaggccgcc ggcttcgcca agtacgtgca gacgggcatc gaactgctgc
     1681 tcaaccagga cgccgtcgtc aacgccacca tgaaggcggg cgccgtgcag gagaccgtca
     1741 ccgtgacgga gaacgcggcg ctgctgaaca cgacgaccgc cgaggtctcg acgcgcttcg
     1801 actcacggcg cctgtcggag ctgcccatca gccccaaccg cagcatctac ctcgtcgcgc
     1861 tctcggc
//
LOCUS       DBNGOH010000449         3228 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4101254, whole genome shotgun sequence.
ACCESSION   DBNGOH010000449 DBNGOH010000000
VERSION     DBNGOH010000449.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3228)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3228)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3228
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4101254"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..676
                     /locus_tag="AC159P_08890"
     CDS             <1..676
                     /locus_tag="AC159P_08890"
                     /inference="COORDINATES: protein motif:HMM:NF014017.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HIR3695608.1"
                     /translation="PPDSTFDGWRRRAPEGFTFSLKLPQEITHEHSLRGAASARALAT
                     FCERARRLGERLAAVLVQLPPQFEATVENARALAEFLPLLPADIRFAVELRDPFWFEE
                     ELLAPLGAHRHVSLALVEGPWVTRERVWRAAAPLLEATDFAYVRWMGARDLTRFDEVQ
                     RERAANLERWAAAIERLSARLPHTYAYFSNYYEGHAPASANKLKRLLGQHVVGPEDLE
                     NQPSLF"
     gene            complement(897..1277)
                     /locus_tag="AC159P_08895"
     CDS             complement(897..1277)
                     /locus_tag="AC159P_08895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695609.1"
                     /translation="MSGRDERAGEAAGGPRPEAAGASRHAAAAGGAHGLAYEQARLAY
                     SIIQTLLEHTRVTQDLVALMAQVIDEDTQRALTETPHWSAYMDSRRAMERAREEIEQF
                     AAEWTRMSREETPPAPDGPPPAHE"
     gene            complement(1274..2437)
                     /gene="ygfZ"
                     /locus_tag="AC159P_08900"
     CDS             complement(1274..2437)
                     /gene="ygfZ"
                     /locus_tag="AC159P_08900"
                     /inference="COORDINATES: protein motif:HMM:NF013720.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CAF17-like 4Fe-4S cluster assembly/insertion
                     protein YgfZ"
                     /protein_id="HIR3695610.1"
                     /translation="MKMSEAVESSEHEESASARRSPLEDAHRRAGASSREQDGWVVAA
                     HYGDAGAEYEAVRGGVGAGLFDLSSRGRVEVGGAEAVQFLNGMLTNDVAKLEDGAWMS
                     VAFPNVQGRLIASARVARLGDRFVFDTEPATHERMLKSLERFTLAGDFRVRDLTSETA
                     MLSLQGAGAAPLVAAVLGEDASRVARGRVSSATFRSSDVSVMRATHTAEDGFDLTIDA
                     GGAGMLWDALSAAGARPAGFDALEVLRVEAGLPRYGVDATEANVVLEVVDEAEAVSYT
                     KGCYVGQEIIARIHWRGHVAKRLAGLVLAREVDAPTAAQLKSCDDARDVGRVTSTVYS
                     PRLRRFVALGLVKYDHLKPGTEVKVSVGDEELCAAHVAELPLVRGSWYAGNDG"
     gene            complement(2434..3099)
                     /locus_tag="AC159P_08905"
     CDS             complement(2434..3099)
                     /locus_tag="AC159P_08905"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:NF015146.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HIR3695611.1"
                     /translation="MRVSEKRGREAFAALVEEAAACRRCPAMCGRSAVLSVRNGRVGA
                     RVMFVGEAPGRQGGDRTRVPFSGDQSGRNFERYLASIGVTRDRIFITNSALCNPRTET
                     GANRKPTRREVCNCSDFLRRQIEVVAPRVVVTLGRVALDALRAVGYHRLTLAESAGKV
                     FEWEGRLLVPLYHPSPQVLASSRREAAQLEDYKAVARALRRADERMAGARAAACRDTA
                     QSL"
BASE COUNT          472 a         1175 c         1087 g          494 t
ORIGIN      
        1 cccgcccgac tcgaccttcg acggctggcg caggcgcgcg cccgaaggct tcaccttctc
       61 gctgaagctg ccgcaggaga tcacgcacga gcactcttta agaggcgcgg cctcggcgcg
      121 cgcgctcgcc acgttctgcg aacgtgcgcg gcggctcggc gaacggctcg ccgccgtgct
      181 cgtccagctc ccgccgcagt tcgaggcgac cgtcgagaac gcgcgcgcgc tcgcggagtt
      241 cctgccgctc ttgcccgcgg acatccgctt cgccgtcgag ctgcgcgacc cgttctggtt
      301 cgaggaggag ctgctcgcac cgctcggggc gcaccggcac gtctcgctcg cgctcgtcga
      361 ggggccgtgg gtcacgcgcg agcgcgtgtg gcgcgcggcg gcgccgctgc tcgaagcgac
      421 cgatttcgcc tacgtgcgct ggatgggcgc gcgcgacctc acgcgcttcg acgaggtgca
      481 gcgcgagcgc gcggcgaacc tcgaacgctg ggccgcggcc atcgagcgtt tgagcgcgcg
      541 cctgccgcac acgtacgcct acttcagcaa ctactacgaa ggccacgcgc ccgccagcgc
      601 caacaagctc aagcggctgc tcgggcagca cgtcgtcggg ccggaggatt tggagaacca
      661 gccttcgctg ttctaaagca agtaatagta gaacgtagaa cgcgcgcgat aactgtagga
      721 gcagggcttg tccccgccca cggttccgcc ttcacttaac gtcgaatcaa acgcatcttg
      781 gcgggccgag gacaagccct gcccctacaa tcttcacgac gtaccgcaga acgtcggagg
      841 gaatgcgtgt gaagtatgat gaaggcgagg atgggaggcg tcgtgatggg cggtctttat
      901 tcgtgcgcgg gcggcgggcc gtccggcgcg ggcggcgtct cttcgcggga catcctcgtc
      961 cactcggcgg cgaattgttc gatctcttcg cgggcgcgct ccatcgcgcg gcggctgtcc
     1021 atgtaggccg accagtgggg cgtctcggtg agcgcccgct gcgtgtcctc gtcgatgacc
     1081 tgcgccatca gcgcgacgag gtcttgcgtg acgcgcgtgt gttcgagcag cgtctggatg
     1141 atcgagtagg cgagccgcgc ctgctcgtag gcgaggccgt gcgcgccgcc cgccgccgcc
     1201 gcgtgccgcg acgcaccagc cgcttcgggc cgcgggccgc ccgccgcttc gcccgcacgc
     1261 tcgtcgcgcc cgctcatccg tcgttccccg cgtaccaact cccgcgcacg agcggcagct
     1321 ccgcgacgtg cgccgcgcac agctcttcgt cgccgacgga gactttgact tccgtgcccg
     1381 gcttgaggtg gtcgtacttg acgaggccga gcgcgacgaa ccgccgcagc cgcggcgagt
     1441 agacggtcga ggtgacgcgc ccgacatccc tcgcgtcgtc gcaggacttg agctgcgcgg
     1501 ctgtgggcgc atccacttcg cgtgcgagga cgagtccggc gagccttttg gcgacgtggc
     1561 cgcgccagtg gatgcgcgcg atgatctcct gcccgacgta gcagcccttc gtgtagctca
     1621 cggcctccgc ctcgtccacg acttcgagca cgacgttcgc ctcggtcgcg tccacgccgt
     1681 agcgcggcag ccccgcctcg acgcgcagga cttcgagcgc gtcgaaccct gcgggccgcg
     1741 cgcccgccgc cgagagcgcg tcccacaaca ttcctgcgcc gcccgcgtca atcgtcaaat
     1801 cgaacccgtc ttcggcggtg tgcgtcgcgc gcatgacgct cacgtccgag ctgcgaaacg
     1861 tcgcggaaga gacgcgcccg cgcgcgacgc gcgacgcgtc ttcgccgagc acggcggcga
     1921 cgagtggcgc cgcgcccgcg ccctgaagcg agagcatggc cgtctcgctc gtgaggtcgc
     1981 ggacgcggaa gtcgcccgcg agcgtgaagc gttcgaggct cttgagcatc cgctcgtgcg
     2041 tggcgggttc ggtgtcgaag acgaagcggt cgccgagtcg cgcgacgcgc gcgctggcaa
     2101 tcaaccgccc ctgcacgttc gggaacgcga cgctcatcca cgcgccgtct tcgagcttcg
     2161 ccacgtcgtt cgtcagcatg ccgttcagga actgcacggc ctccgcgccg ccgacctcga
     2221 cgcgcccgcg cgaagagaga tcgaacagcc ccgcgcccac gccgccgcgc acggcctcgt
     2281 actccgcgcc cgcgtcgccg tagtgcgcgg cgacgaccca cccgtcctgc tcgcgtgagg
     2341 aagcgcccgc gcggcggtgc gcgtcttcga gcggcgaccg ccgcgcgctc gccgactctt
     2401 cgtgctcgct gctctcgacc gcttcgctca tcttcataaa ctctgcgccg tgtctcggca
     2461 ggctgcggcg cgcgcgcccg ccatccgttc gtcggcacgg cgcagcgcgc gcgcgaccgc
     2521 cttgtagtct tcgagctgcg cggcctcgcg gcggctggag gcgagcacct gcgggctggg
     2581 gtgatagaga ggcacgagca gacgcccctc ccactcgaag accttgcccg cgctttcggc
     2641 cagtgtgagt cgatgatagc caacggcgcg gagcgcatca agcgccaccc gcccgagcgt
     2701 gacgacgacg cgcggggcca cgacctcgat ctgacggcgc aggaagtccg agcagttgca
     2761 gacctcgcgg cgcgtcggct tgcggttcgc gcccgtctcc gtgcgcgggt tgcagagcgc
     2821 ggagttggtg atgaagattc ggtcgcgcgt gacgccgatg gaggcgaggt agcgctcgaa
     2881 gttgcgcccc gactggtcgc ccgaaaaagg gacgcgcgtg cggtcgccgc cctgcctgcc
     2941 cggcgcctcg ccgacgaaca tcacgcgcgc gcccacgcgc ccgttgcgca cgctcaagac
     3001 cgcgctccgc ccgcacatcg ccgggcacct gcggcacgcc gccgcctcct cgacgagcgc
     3061 cgcgaacgcc tcgcgcccgc gcttctcgga aacgcgcatt ccgtaattcc ctctcacgca
     3121 acagagctta acaagacgaa tggcgaatgc gaaatgggga acggaaatca gaagtcggaa
     3181 gtcggaataa gagccgtctt cattccgact tccgacttcc gacttccg
//
LOCUS       DBNGOH010000450         2218 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_801299, whole genome shotgun sequence.
ACCESSION   DBNGOH010000450 DBNGOH010000000
VERSION     DBNGOH010000450.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2218)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_801299"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(29..1213)
                     /locus_tag="AC159P_08910"
     CDS             complement(29..1213)
                     /locus_tag="AC159P_08910"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HIR3695612.1"
                     /translation="MTDKDLSRPFRPAARLRGVEKSMIRQVFDRARPNSINLGLGEPD
                     LPTPDVIRREAARVVLEEQNGYTTHAGLPALRERVAADYPPLKLEPEQVIITAGSQEA
                     LYLALLSLVDEGDEVLIPDPGFVAYPTIVRMAGGTPVRYRLPAAHGFRFDAEALRRAL
                     TPRTRAVVLISPSNPTGRVLSREDLAEMADALKDSDAYVVSDEIYRELYYGGERPASI
                     SDFLQERTVVIGGLSKSMSMTGWRLGWLCGDANVLKSALVLHGYVTTCASTVSQKAAH
                     AAWTEEAAEARARHRSIFRARRDHLDALLSTELKLRAVRPDGAFYTMVDVSAYGSSLQ
                     VAERLLEHGVITVPGAAFGAEGEGFLRVSFCADLPVITEGVRRMGEALQSKSEVGTQN
                     DE"
     gene            complement(1395..>2218)
                     /locus_tag="AC159P_08915"
     CDS             complement(1395..>2218)
                     /locus_tag="AC159P_08915"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HIR3695613.1"
                     /translation="ERVEALAAKQAGAFRDEDREAFAAFKRALNRGEVRAAERDAEGT
                     WRVNAWVKQGILAGFRMGALADMSAHSSLRFFDKDTYPVRATVAEDNVRIVPGGSSVR
                     DGAYLAPGVVCMPPMYVNVGAYVDEGTMIDSHALVGSCAQVGKRVHLSAAAQIGGVLE
                     PIGAVPVVVEDDVMVGGGCGIYEGTIVREGAVLAAGTILTGSTPVYDLVRGEIYRRTD
                     AGPLEIPAGAVVVPGSRRVENERGRAWGLSLYAPVIVKYRDEKTERAVKLEDYLR"
BASE COUNT          358 a          751 c          728 g          381 t
ORIGIN      
        1 agaacgatga ataaagagca acttgtcttt attcatcgtt ctgcgttccg acttccgact
       61 tgctttgcaa cgcttctccc atccgccgca cgccttcggt aatgacgggc aggtcggcgc
      121 agaacgagac gcgcaggaag ccttcgcctt cggcgccgaa ggctgcgccg gggacggtga
      181 tgacgccgtg ttcgagcaga cgctcggcga cctgcaagga gctgccgtaa gcgctcacgt
      241 caaccatcgt gtagaaggcg ccgtcgggcc ggacggcgcg caatttcagt tcggtcgaga
      301 gcagcgcgtc gaggtggtcg cggcgcgcgc ggaagatcga gcggtgacgc gcgcgtgcct
      361 ccgcggcctc ttcagtccac gcggcgtgcg cggccttctg cgagacggtc gaggcgcacg
      421 tcgtgacgta gccgtgcagg acgagcgcgc tcttgagcac gttggcgtcg ccgcagagcc
      481 agcccagccg ccagcccgtc atgctcatcg atttggagag gccgccgatg acgaccgtgc
      541 gttcttgaag gaagtccgaa atagacgcgg gccgctcgcc gccgtagtaa agctcgcggt
      601 aaatctcgtc gctgacgacg taggcgtccg agtctttcaa ggcgtcggcc atctcggcga
      661 ggtcttcgcg cgagagcacg cggcccgtcg ggttcgaggg gctgatgagg acgacggccc
      721 tggtgcgcgg agtgagcgcg cggcgcagcg cttcggcgtc gaaccggaag ccgtgcgccg
      781 cgggaagccg atagcggacg ggcgtgccgc ccgccatgcg gacgatggtc gggtaggcga
      841 cgaagccggg gtctggaatc aagacctcgt cgccttcatc gacgagcgag agcagcgcga
      901 ggtacagggc ctcctgcgag cctgccgtga tgatgacctg ttcgggttcg agcttgagcg
      961 gcgggtagtc ggcggccacg cgctcgcgca aagccggcag ccccgcgtgc gtggtgtagc
     1021 cgttctgctc ttcgaggacg acgcgcgccg cctcgcggcg aatcacgtcg ggcgtcggca
     1081 agtccggctc gccgagtccg aggttgatgc tgttgggccg cgcgcggtcg aagacctgcc
     1141 ggatcatgct cttctcgacg ccgcgcagcc gcgccgccgg tcggaatggc cgtgataagt
     1201 ctttatccgt cacagggaat gttatcggct cgttttcttt agcctgagtg tccggcagct
     1261 tcatttcacc acagaggaca cagaggtttc acggagagca cagagaaagt cattcttatt
     1321 cttctctgcg tcctctgtga aacctctgtg tcctctgtgt tgaaatccaa cgtgtgcaat
     1381 gctcaatccg cgagtcagcg caggtagtct tccagcttca ccgcgcgctc cgtcttctcg
     1441 tcgcggtact tgacgatgac gggcgcgtag agcgagaggc cccacgcgcg gccccgctcg
     1501 ttctcgacgc ggcgcgagcc gggcaccacc accgcgcccg ccggaatctc aagcggcccc
     1561 gcgtcagtcc gtcgataaat ctcgccgcgg accaaatcgt aaaccggcgt cgagccggtc
     1621 aggatcgtcc ccgccgcgag caccgcgccc tcgcgcacaa tcgtcccttc gtagatgccg
     1681 cagccgcctc cgaccatcac gtcgtcctcg accacgaccg gcaccgcgcc gattggttcg
     1741 agcacgccgc cgatctgcgc cgccgccgag aggtgcacgc gcttgcccac ctgcgcgcac
     1801 gagccgacga gcgcgtggct gtcgatcatc gtcccctcgt ccacgtacgc gccgacgttc
     1861 acatacatcg gcggcatgca cacgacgccc ggcgcgagat aagctccgtc gcgcacgctc
     1921 gacccgcccg gcacgatccg cacgttgtct tccgcaacgg tcgcgcgcac agggtacgtg
     1981 tccttgtcga agaaacgaag gctgctgtgc gcgctcatgt ccgcgagcgc gcccatgcgg
     2041 aagcccgcga ggatgccctg cttgacccac gcgttgacgc gccaagtgcc ttccgcgtcg
     2101 cgctcggcgg cgcgcacctc gccgcggttg agcgcccgct tgaacgcggc gaaggcttcg
     2161 cggtcttcat cgcggaaggc gcccgcctgt ttggcggcca gcgcctccac gcgctcac
//
LOCUS       DBNGOH010000451         6097 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3919343, whole genome shotgun sequence.
ACCESSION   DBNGOH010000451 DBNGOH010000000
VERSION     DBNGOH010000451.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6097)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6097)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6097
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3919343"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..4555
                     /locus_tag="AC159P_08920"
     CDS             <1..4555
                     /locus_tag="AC159P_08920"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HIR3695614.1"
                     /translation="ECAGDRSLRAEVESLIRWHEKGGDFMEAPAARMAAGLAAGAECE
                     LEEGQLVGSYRVAGALGEGGMGRVYLAQDTRLGRKVALKILPASFTSDEERVRRFEQE
                     ARAASALNHPNILTIHEIGDAEGGARFIATEFVEGATLREQLRRPSASSSSSSSSSSS
                     SSPDLREALEVATQVAAALTAAHAAGIVHRDIKPENVMVRPDGYVKVLDFGLAKPTRR
                     AAGGSSSAGDTDDSDSFLRVFVNTQPGVVMGTVAYMSPEQARGLDVDARTDIWSLGVV
                     LYEMVAGRAPFKGPTPSDVMAAVLQREPAPLSQVTVGVPSELERIVTKALAKDREERY
                     QTAKDLLIDLRRLKQRMEFEAEMGRSAAAKRAGEAAAPLARPHQVKQPHAPSAHLSGE
                     QLKQVTVLFADLYGLTALTETLDAEEVRDHMREVWNRVDAAVMEHGGTVDRHMGGEVM
                     ALWGAHRSHEDDPENAVRAALRMREEVAAYISTALAGEVLAAGGGAFEDEGAAGGNEV
                     GAEAGLDAALMRVGVNTGAVLLGEVGARGELTATGAAVNVAQRLKQSAPAGSILVSHD
                     TYRHVRGIFDVRELELMNVRGGGEPVRTYRVERAKPRAFRVRTRGVEGVETRMIGRTG
                     ELARMRDALHTVAEDRELQVITIVGDAGLGKSRLLYEFSNEVELGAGRAPEVFQGRAS
                     QWARGLPYALVRDVFSFRFRIQDSDAPAVAREKLERGMAETWGEGREAEMRAHFIGHL
                     IGLDFSGSPHLAGILDDAKQVRGRAFHYAAQFFSAVAREATTLLYLDDLHWADDGSLD
                     FVDYLARACAASPLLVLCFARPDLLERRPAWGEGLECHARLTLQPLTKRESRQLVEEI
                     LRHARAIPHALRELVVSAAEGNPFYVEELIKMLIDQKVILPAAGHWRVDASRLAEVRV
                     PPTLTGVLQARLDALSVWEKTVLQRASVIGREFWEGALEEFSRTALARGELGEGDTAS
                     ALELLRGKELVYRREASAFTGTREYIFKHAILRSVTYENVLKRERRRLHKETAAWLIE
                     RSGARVDEYAAVIAEHYERAREAASAAEWYGRAGNQARASYAPEAAIGFYRKALDFMR
                     DVERAGATAPSSTREAAATGVAAAAHGASSLRVQAIKWYGGLGEVLAMQARYDEAVEA
                     YERMREAAEEAGDRVAEARAWNGLTAVREYQGNNRAALESAQRAERLALEAGEAAGAK
                     AELAVALNRQGLASHRLGDAAAVMKFGQQILSLSEGMPEGVGASARANGLRLVGVAHE
                     MSGRFARADEYVEQSLSLLKELGDRRNVGFMLNNLGVIAHLRGDFEEAVRRYEESLAI
                     FREIGERTCELPTLGNLAGAQVGMGSYAAAEANLREAVQMTGPAGHFALSMVYCYLAE
                     SFWGQGKIDDALETARTALELGLRTENQDYIASAWRALGLVASRLAEPIQVEGRERTA
                     RDCFAESLRVFTEMGAEAERARTLRDWARYESERGESERGAEMWHESREIFTRLKMER
                     ELERMPPELPPETATGGDARAGV"
     gene            complement(5066..5437)
                     /locus_tag="AC159P_08925"
     CDS             complement(5066..5437)
                     /locus_tag="AC159P_08925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695615.1"
                     /translation="MLKTLLLAAVAFAALAFVPAQEKKAGTTKITGYLIDNLCAEASQ
                     GGEEEEAEGHMVSCALMEACQRSGYAVISEGKTYKLDEKGNKLALKVLRDTRVKQGLA
                     VTVIGALDGDTLHADTVTESR"
     gene            complement(5478..>6097)
                     /locus_tag="AC159P_08930"
     CDS             complement(5478..>6097)
                     /locus_tag="AC159P_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HIR3695616.1"
                     /translation="VSAEIHKRNPHSSEAQDYINQSFEEAWRVAEETHDPERESNEPV
                     ESFLARVIAYAFESVVESHLIQPTFIYDFPKAISPLSKASPEKPAIAERFELFAAGME
                     LANGFSELNDPVEQHQRFLEQTEQRERGDDEAMQMDLDYVRALSYGMPPAAGIGIGVD
                     RLTMLLTNRRSIRDVILFPHMRPRREAEAEAETTPDAAETDSPDA"
BASE COUNT          954 a         2010 c         2204 g          929 t
ORIGIN      
        1 cgagtgcgcg ggcgaccgga gtttgcgcgc ggaggtcgag tcgctgatcc gctggcacga
       61 gaagggcggc gacttcatgg aagccccggc ggcgcggatg gcggcggggc ttgcggcggg
      121 cgcggaatgc gagctggagg aggggcagct cgtcgggtcg taccgcgtgg cgggcgcgct
      181 cggcgagggc gggatgggga gggtctatct cgcgcaggac acgcggctcg ggcgcaaggt
      241 cgcgttgaag attctgcccg cctccttcac gagcgacgag gagcgcgtcc gccgcttcga
      301 gcaggaggcg cgcgccgcct cggcgctgaa ccacccgaac attctcacca tccacgagat
      361 cggcgacgcg gagggcggcg cccgcttcat cgccaccgag ttcgtcgagg gcgcgacgct
      421 gcgcgaacaa ctgcgccgcc cgtcggcgtc gtcatcgtcg tcatcgtcgt catcgtcgtc
      481 ttcctctccc gatttgcgcg aggcgctcga ggtcgccacg caggtcgccg ccgcgctcac
      541 cgccgcgcac gccgcgggga tcgtccaccg cgacatcaag cccgagaacg tgatggtcag
      601 gcccgacggc tacgtgaagg ttctggactt cggactcgcc aagccgacgc gccgcgccgc
      661 gggcggttcg tcgtcggcgg gcgacacgga cgacagcgac tcgttcctgc gcgtcttcgt
      721 caacacgcag cccggcgtcg tgatgggcac cgtggcctac atgtcgccgg agcaggcgcg
      781 cggcctcgac gtggacgcgc ggacggacat ctggtctctg ggcgtcgtgc tctacgagat
      841 ggtcgcgggc cgcgcgccgt tcaaggggcc gacgccgagc gacgtgatgg ccgccgtctt
      901 gcagcgcgag ccggcgccgc tctcgcaagt cacggtgggc gtgccttcgg agctggagcg
      961 catcgtgacg aaggcgctcg ccaaagaccg cgaggagcgt taccagacgg cgaaggactt
     1021 gctcatcgac ctccggcggc tcaagcagcg gatggagttc gaggccgaga tgggccgctc
     1081 cgccgccgcc aagcgcgcgg gcgaagccgc cgcgcccctc gcccggcctc atcaagtcaa
     1141 acagccgcac gcgccctcgg cacacctctc cggcgaacag ctcaagcagg tgacggtact
     1201 cttcgcggac ctctacgggc tgacggcgct gaccgaaacg ctcgacgccg aggaggtgcg
     1261 cgaccacatg cgcgaggtct ggaaccgcgt ggacgcggcg gtgatggagc acggcggcac
     1321 cgtcgatagg cacatgggcg gcgaggtaat ggcgctgtgg ggcgcgcacc gctcgcacga
     1381 ggacgacccg gagaacgccg tgcgcgccgc gctccgcatg cgcgaagagg tcgccgcgta
     1441 catctcgacg gcgctcgcgg gcgaagtgct cgcggcaggc ggcggcgcgt tcgaagatga
     1501 aggcgccgct ggcgggaacg aggtcggcgc ggaggcgggc ctcgacgcgg cgttgatgag
     1561 ggtcggcgtg aacacgggcg cggtgctgct cggcgaggtc ggcgcgcgcg gcgagttaac
     1621 ggcgacgggc gcggcggtca acgtcgcgca gcgcttgaag cagtcggcgc ccgccggctc
     1681 gatcctcgtc tcgcacgaca cgtaccggca cgtgcgcggc atcttcgacg tgcgcgagct
     1741 ggagctgatg aacgtgcgcg gcggcgggga gccggtgcgc acgtaccgcg tcgagcgcgc
     1801 caagccgcgc gccttccgcg tccgcacgcg cggcgtcgag ggcgtcgaga cgcgcatgat
     1861 cgggcgcacg ggcgagctgg cgcggatgag ggacgcgctg cacacggtcg ccgaagaccg
     1921 cgagcttcag gtcatcacca tcgtcggcga cgcggggctg ggcaaatcgc gcctgctgta
     1981 cgagttcagc aacgaggtcg agctgggggc cgggcgcgcg ccggaggtct ttcaggggcg
     2041 cgcgagccag tgggcgcggg ggctgccgta cgcgctcgtg cgcgacgtgt tctcgttccg
     2101 cttccggata caggacagcg acgcgcccgc ggtcgcgcgc gaaaaactgg aacgcggcat
     2161 ggccgagacg tggggcgagg ggcgcgaggc agagatgcgc gcgcacttca tcggccacct
     2221 catcggcctc gacttttcgg gcagcccgca cctggcgggc atcctcgacg acgcgaagca
     2281 ggtcaggggg cgcgccttcc actacgcggc gcagttcttc tcggcggtcg cgcgcgaagc
     2341 gacgacgctg ctctacctcg acgacctgca ctgggccgac gacggctcgc tcgacttcgt
     2401 cgattacctc gcgcgcgcgt gcgccgcctc gccgctgctc gtcctctgct tcgcgcgccc
     2461 cgacctgctg gagcgccggc ccgcgtgggg cgaaggtctc gaatgccacg cgcgcctgac
     2521 gctgcaaccg ctgacgaagc gcgagagccg ccagctcgtc gaggagatac tccggcacgc
     2581 gcgggccatc ccgcacgcgc tgcgcgagct ggtcgtgagc gcggcggagg gcaacccgtt
     2641 ctacgtcgaa gagctgatca agatgctcat cgaccagaag gtgatcctgc ccgccgcggg
     2701 ccactggcgc gtggacgcga gccgcctcgc ggaggtgcgc gtgccgccga cgttgacggg
     2761 cgtgctgcaa gcgcgtctcg acgcgctctc ggtgtgggag aagacggtgt tgcagcgcgc
     2821 ctcggtcatc gggcgcgagt tctgggaggg ggcgctcgaa gagttctcgc gcaccgccct
     2881 cgcgcgcggc gagctgggcg agggcgacac ggcttcggcg ctcgaactgc tgcgcggcaa
     2941 ggagctggtc taccggcgcg aggcgtcggc cttcacgggc acgcgcgagt acatcttcaa
     3001 gcacgccatc ctgcgcagcg tcacctacga gaacgtgctg aagcgcgagc ggcgccggct
     3061 gcacaaagag accgccgcgt ggctcatcga gcggagcggc gcgcgcgtgg acgaatacgc
     3121 cgccgtcatc gccgagcact acgagcgcgc gcgcgaggcc gccagcgccg ccgagtggta
     3181 cgggcgcgcg ggcaaccagg cgcgcgcctc ttacgcgccc gaggccgcca tcggcttcta
     3241 ccgcaaggcg ctcgacttca tgcgcgatgt cgagagagcc ggtgcaacgg ctccttcgtc
     3301 aacgcgcgag gcggcggcaa cgggcgtcgc ggcggcggcg cacggcgcgt cgtcgctccg
     3361 cgtgcaggcg atcaagtggt acggcggcct cggcgaagtg ctcgccatgc aggcgcgcta
     3421 cgacgaggcg gtcgaggctt acgagcggat gcgcgaggcg gcggaagagg cgggcgaccg
     3481 cgtcgcggag gcgcgcgcgt ggaacgggct gaccgccgtg cgcgagtatc agggcaacaa
     3541 ccgcgcggcg ctggagagcg cgcagcgcgc cgaacggctc gcgctcgaag cgggcgaggc
     3601 ggcgggcgcg aaggccgagc tggcggtcgc cctgaaccgt cagggtctcg cttcgcatcg
     3661 cctcggcgac gcggcggcgg tcatgaagtt cggccagcag attttatctc tgagcgaggg
     3721 gatgccggag ggcgtcggcg cgtcggcccg cgcgaacggc ctgcggctgg tcggcgtggc
     3781 gcacgagatg tcgggccgct tcgcgcgggc ggatgagtac gtcgagcagt cgctctcgct
     3841 cctgaaggag ctgggcgacc gtcgcaacgt cggcttcatg ctcaacaacc tcggcgtcat
     3901 cgcgcacctg cgcggcgact tcgaagaggc cgtgcggcgc tacgaagagt cgctcgccat
     3961 cttccgcgag atcggcgagc gcacctgcga gctgccgacc ctgggcaacc tcgcgggcgc
     4021 gcaggtcggg atgggcagct acgccgccgc cgaggcgaac ctgcgcgagg ccgtccagat
     4081 gaccggcccc gccggccact tcgcgctctc gatggtctac tgctatctgg ccgagagctt
     4141 ctgggggcag ggcaaaatcg acgacgcgct ggagacggcg cgcaccgcgc tcgaactcgg
     4201 actgcggacg gagaaccagg actacatcgc cagcgcttgg cgcgcgctcg gcctcgtcgc
     4261 ctcgcgcctc gcggagccga ttcaagtcga aggccgcgag cgcaccgcgc gcgactgctt
     4321 cgcggagagc ctgcgcgtct tcacggagat gggcgccgag gccgagcgcg cgcgcacgct
     4381 gcgcgactgg gcgcgctacg agagcgagcg cggcgagagc gagcgcggcg cggagatgtg
     4441 gcacgagtcg cgcgagattt tcacgcggct gaagatggag cgcgagctgg aacgcatgcc
     4501 gccggaactg ccgccggaga cggcgacggg cggcgacgcg cgggcgggcg tgtagagggc
     4561 ggcaggcggg cgttgacgaa cgcacgccgt ctccgtccgc ggggcgtctt aaaagttgaa
     4621 gatcgtcggc ccgtattcag tggaagtcat ggcgtttgct ccgtaccgtc cgaagttgag
     4681 gagtcggtct tgtgccgggg atgatggccg cgacagcttg cggcccgccg tcggaaacgg
     4741 gccggcgggg tgtggacgat tgcggaatgc gtgcgcaccg gcagcgggac agatctcccc
     4801 gtgtctgttc tccgcaccgc ggcgcacgca ccccgcaatc ttccggccct gatttggccc
     4861 acaacggaag agccttgtgg ccgccgtcag cacatcgcgt ccggccaaca aacctcttct
     4921 catcgggtaa cgcgtattcc gtcggcggac tcccaacagg gcggagaaaa aaagagggga
     4981 aagggtgagg gggaaagggg aaagggagaa gacccgcttg ctttctccct ttcccctttc
     5041 cccctcaccc tttccccttc gcgcgtcagc gcgattcggt gacggtgtcg gcgtggagcg
     5101 tgtcgccgtc gagggcgccg atgacggtga cggcgaggcc ctgcttgacg cgcgtgtcgc
     5161 gcaggacttt gagggcgagc ttgttgccct tctcgtcgag cttgtaggtc ttgccctcgg
     5221 agatgacggc gtaaccgctc ctctggcacg cctccatcag cgcgcacgag accatgtgcc
     5281 cctcggcctc ctcctcctcg ccgccctgcg acgcctcggc gcacaggttg tcgataagat
     5341 agcctgtgat cttcgtcgtc cccgccttct tctcctgcgc ggggacgaag gccaaagcgg
     5401 cgaaggcgac ggcggcgagc agcaatgtct tcaacatcat tcgggggctt actccttttg
     5461 aattaacgag cggttcttca ggcgtctggg gagtctgtct ccgcggcgtc cggcgttgtc
     5521 tcggcttcgg cttcggcctc gcggcgcggg cgcatgtgcg ggaatagtat cacgtcgcgg
     5581 atggaacggc ggttcgtcaa cagcatcgtc agcctatcaa ccccgatgcc gatgcccgcg
     5641 gcgggcggca tcccgtacga caacgcccgc acgtaatcca aatccatctg catcgcctcg
     5701 tcatcacccc gctcccgctg ctccgtctgt tccaaaaaac gctggtgctg ctcaacgggg
     5761 tcgttcaact ccgagaagcc gttcgccaac tccatccctg cggcgaacaa ctcgaagcgc
     5821 tcggctatag ctggtttttc gggcgacgct ttcgagaggg gagaaatagc tttaggaaaa
     5881 tcatatatga atgttggttg aatcaaatga ctttccacta cgctctcgaa cgcgtaggcg
     5941 atcactcgcg ctaaaaaact ctccaccggt tcattagatt cacgttcggg gtcatgagtc
     6001 tcctctgcta cccgccaagc ttcttcaaaa ctttgattga tgtaatcttg tgcttcgctg
     6061 ctatgcggat ttcgtttatg tatctccgct gagacat
//
LOCUS       DBNGOH010000452         3432 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4650897, whole genome shotgun sequence.
ACCESSION   DBNGOH010000452 DBNGOH010000000
VERSION     DBNGOH010000452.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3432)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3432)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3432
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4650897"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..357)
                     /locus_tag="AC159P_08935"
     CDS             complement(<1..357)
                     /locus_tag="AC159P_08935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695617.1"
                     /translation="MRPKIGTESSSVVRSPAATGLLLLFLICHAFLVSATHFHRDVRP
                     GAQTTTVGLSAGSDEGTEPSSVAFNHAQCLLCRLQRNFICDLEQGIPSMPAPPADTLR
                     RESLTAPFHSRTFYLTH"
     gene            691..1719
                     /locus_tag="AC159P_08940"
     CDS             691..1719
                     /locus_tag="AC159P_08940"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HIR3695618.1"
                     /translation="MPFQRAAARRRRLVSPRSARSPLFVLLSITFALFAPAPRARAQE
                     APPQSDEVVRVRTDLVTVPVVVTDARGRRVAGLTRDDFELLDEGLRVETTYFASGAER
                     VAMLFLLDASGSTRDIVTQQRETALAIFTRFGPRSRVAAMHFREQPELVLPFTTDLKK
                     ARGAFRPEAIPDRLTAIFDAALAAVRAFASPPQDPLERRIVVLISDGLDTASRTRAAA
                     VIDAANAAGVTFYVIHLPLFMARDGGLAMRRPSKGFRELAEKTGGQYFVVGDAASSLD
                     PRAEYDLSAVFGVISDDLRGQYVLGYHAGEGAQRDGRFRRVEARLAKKDKRRLRVRQL
                     REGYALKP"
     gene            1798..>3432
                     /gene="purH"
                     /locus_tag="AC159P_08945"
     CDS             1798..>3432
                     /gene="purH"
                     /locus_tag="AC159P_08945"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HIR3695619.1"
                     /translation="MNAPNKEGLRTIRRALLSVSDKSGLIDFARALAGFGVEILSTGG
                     TAKALREAGLEVRDVSDVTGFPEMLDGRVKTLHPRVHGGILAVRDNEEHARALAEHGI
                     EPIDMVVVNLYPFEQTVAREGVTLEEAVEQIDIGGPAMIRSAAKNHADVAVVTAPELY
                     AGILDEMRLCGGSLSARTRSQLALLAFSRTASYDSSISLFLSIHASFSDKPEWLVLFS
                     EYIAHSFENQGQLVDAALPEDYEDGEGRDELGGESFKLTLDKLNGLRYGENPHQRAAL
                     YDTGEGDEGVAQAELLGGKEMSFNNYVDADAAWQLVCDIDETACAIIKHMNPSGVGTG
                     ATLAEGYRRALATDPVSAFGGIVAFNRTVDEEAARAAAEIFTEVVIAPDFDDAAIEVL
                     KSKKNLRILRAGRVQQPRGLDFKRITGGLLMQTRDTHRLTRDELKVVTKRAPSEEEIK
                     ALLFAWTVSKHTKSNAIVYAREGQTVGVGAGQMSRVDSVRIGAMRAQLPLAGSVLASD
                     AFFPFRDGVDEAAKHGITAVIQPGGSVRDAEVIAAAD"
BASE COUNT          566 a         1185 c         1124 g          557 t
ORIGIN      
        1 gtgtgtcagg tagaaggtgc gggagtgaaa cggagccgtg agactttcac ggcggagggt
       61 gtccgcgggc ggcgcgggca tcgaggggat gccctgctca aggtcgcaga tgaagttgcg
      121 ctggagccgg cacaggagac actgcgcgtg attgaacgcg acgctcgacg gctctgtgcc
      181 ttcgtcgctt cccgcgctca agccgacggt cgtcgtctgc gcgccggggc gtacgtcgcg
      241 gtggaagtgc gtggcgctga cgaggaaggc gtggcagatc agaaagagca gcaacaggcc
      301 ggtcgccgct ggcgatctga caacactgct gctttcggtg ccgattttcg gtctcaagtt
      361 gctgcttccg gctgagagtt accgggtaca atataccacc gcgcacgccg acgttgaagc
      421 agcgcggcgg gcgcggcgga acctgcgggc ctgctcccgc gtaatatttc tcgaatggac
      481 gaacagtagg agccgacagc agacgggccg ctgcgcgttg acaccccgct ccgcgcgccg
      541 ataaaatgcc ttccctgctg gcaatcgtcc cgttgctgct ctgccggaga taacgttcgt
      601 gagcctgatt aaagtcatcg tcatcgccat cgcgctgatc ctgagcctgc tcctgttcgg
      661 gcgcttcttc ttttaagtcc gacagaaggc atgccctttc aacgcgcagc ggcccgccgc
      721 agacgactcg tctcgccgcg ctcggcccgc tcccctctct tcgttcttct ctccatcacg
      781 ttcgcgctgt tcgcccccgc gccgcgcgcc cgcgcgcagg aggcgccgcc gcaaagcgac
      841 gaggtggtgc gcgtccgcac ggacctcgtg accgtgcccg tcgtcgtcac ggatgcgcgc
      901 gggcgtcgcg tcgcgggttt gacgcgcgac gacttcgagc tgctcgacga agggctgcgt
      961 gtcgagacca cctacttcgc ctccggtgcg gagcgcgtgg cgatgctctt cctgctcgac
     1021 gcgtcgggca gcacgcgcga catcgtgacg cagcagcgcg agacggcgct cgccatcttc
     1081 acgcgcttcg gcccacgctc gcgcgtcgcc gcgatgcact tccgcgagca gcccgaactc
     1141 gtcctcccct tcacgaccga cctcaagaag gcgcgcggcg cctttcgccc cgaggccatc
     1201 cccgaccgtt tgaccgcgat cttcgacgcc gcgctcgccg ccgtccgcgc gttcgcgtcc
     1261 ccgccgcaag accctttgga gcggcgcatc gtcgtcctca tcagcgacgg gctggacacc
     1321 gcgagccgga cgcgcgccgc cgccgtcatc gacgcggcga acgcggcggg cgtcacgttc
     1381 tacgtgattc acctgccgct cttcatggca cgcgacggcg ggcttgccat gcgccgcccg
     1441 tcgaaaggtt ttcgtgagct ggcggagaag acgggcgggc agtatttcgt cgtcggcgac
     1501 gccgcctctt cgctcgaccc gcgcgccgag tacgacctca gcgccgtctt cggcgtcatt
     1561 tccgacgacc tgcgcggcca gtacgtgctc ggctaccacg cgggcgaagg ggcgcagcgc
     1621 gacgggcgct tccgccgcgt cgaggcgcgg ctggcgaaga aagacaaacg tcgcttgcgc
     1681 gtgcgccagt tgcgcgaggg ctacgctttg aagccgtgac aggtgacggg cggcgggcgg
     1741 cgggttagaa tgcgttacct tgctcttcca ttttcatacg atccatcctc agcgaacatg
     1801 aacgcaccaa acaaggaagg actgcggacg attcgccgcg cgctcctgag cgtgtcggac
     1861 aagagcggac tcatcgactt cgcgcgcgcg ctcgccggct tcggcgtcga gattctgagc
     1921 acgggcggca cggcgaaggc gctgcgcgag gcgggcctcg aagtccgcga cgtgtcggac
     1981 gtgaccggct ttcccgagat gctcgacggt cgcgtgaaga cgctccaccc gcgcgtccac
     2041 ggcggcatcc tcgccgtgcg cgacaacgag gagcacgcgc gcgcgctcgc cgaacacggc
     2101 atcgagccga tagacatggt cgtcgtcaac ctctacccgt tcgagcagac ggtcgcgcgc
     2161 gagggcgtca cgctcgaaga ggccgtcgag cagatagaca tcggcggccc ggcgatgatt
     2221 cgttcggcgg cgaagaacca cgctgacgtg gccgtcgtca ccgcgcccga actgtacgcc
     2281 gggatacttg acgagatgcg actgtgcggc ggatcgctct cggcgcggac gcgctcgcaa
     2341 ctggcgctgc tcgccttctc gcgcacggcc tcttacgact cgtccatctc gctcttcctt
     2401 agcattcacg cctcgttctc ggacaagccc gaatggctcg tgctgttcag cgaatacatc
     2461 gcgcactcct tcgagaatca ggggcagttg gttgacgccg cgctccccga agactacgag
     2521 gacggcgagg ggcgcgacga actcggcggc gaatctttca aactcaccct cgacaagctg
     2581 aacggcctcc gctacggcga gaacccgcac cagcgcgccg cactctacga caccggcgag
     2641 ggcgacgaag gcgtagcgca ggccgaactg ctcggcggca aggagatgtc cttcaacaac
     2701 tacgtcgatg cggacgccgc gtggcagctc gtctgtgaca tcgacgagac ggcctgcgcc
     2761 atcatcaagc acatgaaccc ttcgggcgtc ggcacgggcg cgacgctcgc cgaaggctac
     2821 cgccgcgcgc tcgccacaga ccccgtctct gccttcggcg gcatcgtcgc cttcaaccgc
     2881 acggttgacg aagaggccgc gcgcgccgcc gccgagattt tcacggaggt cgtcatcgcc
     2941 cccgacttcg acgacgccgc catcgaagtg ctgaagtcga agaagaacct gcgcatcctc
     3001 cgcgcggggc gcgtgcaaca accgcggggg ctggacttca agagaatcac cggcggcctg
     3061 ctcatgcaga cgcgcgacac gcaccggctg acgcgcgacg agttgaaggt cgtgacgaaa
     3121 cgcgcgccga gcgaagaaga gatcaaagct ctgctgttcg cgtggacggt ctcgaagcac
     3181 acgaagtcga acgccatcgt ctacgcgcgc gaggggcaga ccgtgggcgt cggcgcgggg
     3241 cagatgtcgc gcgtggactc cgtccgcatc ggcgcgatgc gcgcgcagtt gccgctcgca
     3301 ggctccgtgc tcgcgtcgga cgccttcttc cccttccgcg acggcgttga cgaagccgcc
     3361 aagcacggca tcaccgccgt catccagccc ggcggctccg tccgcgacgc cgaagtaatc
     3421 gccgccgcgg ac
//
LOCUS       DBNGOH010000453         3143 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1352963, whole genome shotgun sequence.
ACCESSION   DBNGOH010000453 DBNGOH010000000
VERSION     DBNGOH010000453.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3143)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3143)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3143
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1352963"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..499)
                     /locus_tag="AC159P_08950"
     CDS             complement(<1..499)
                     /locus_tag="AC159P_08950"
                     /inference="COORDINATES: protein motif:HMM:NF036516.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M61 family metallopeptidase"
                     /protein_id="HIR3695620.1"
                     /translation="MSTHARRALVRPLVRPLVLLALFAFALPCAAQEQASAPYRIAYT
                     LSMPRPETHLFEVRVEVDGVGGASHVDFQMPRWSPGRYAVFDFAKNVQEFSAENFCGG
                     ATCVLTDLPVTRLDTQTWRVGTLGGMTTHVRVRYKVFADDLSGTFSQLDERHANFNGG
                     SVFMYV"
     gene            811..2070
                     /locus_tag="AC159P_08955"
     CDS             811..2070
                     /locus_tag="AC159P_08955"
                     /inference="COORDINATES: protein motif:HMM:NF014342.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA deacylase
                     activity [Evidence IEA];
                     GO_function: GO:0002196 - Ser-tRNA(Ala) deacylase activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein"
                     /protein_id="HIR3695621.1"
                     /translation="MGNTERLYYDDPHLTEFDARVLAVESASGGRLAVRLDRTAFYPT
                     GGGQPTDTGTLGAARVVECVDEEEGGVLHLVEGEAPAAGALVNCRVDWPRRLEHIQHH
                     TGQHILSQAFVELHGAETRSFRMTHDAAEIDVELAEPSDEKIERAIDRANEIIWADRA
                     VRIHHATPDEAAAMPLRKDSAREGALRVIEIDNFDFSPCGGTHALRTGEVGLVAVRQW
                     ERAKGLTRITFVAGARALADYRRANHAARAAAAAFSAARDEAPAAVARLLEEHKHALR
                     RLRELSALAARVEARDLYEEAARPSSAAAPHASTDGPVIVARVFENRDAEELKQLALA
                     LAKHPRAVALLGSRDDAAGVRVVFARAPELAHDMNALVREAGAHLEGRGGGRSDLAQG
                     GGRRADRIDEVIAAAAARISSSRAEQK"
     gene            2138..2214
                     /locus_tag="AC159P_08960"
     tRNA            2138..2214
                     /locus_tag="AC159P_08960"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2172..2174,aa:Ala,seq:cgc)
     gene            complement(2221..2895)
                     /locus_tag="AC159P_08965"
     CDS             complement(2221..2895)
                     /locus_tag="AC159P_08965"
                     /inference="COORDINATES: protein motif:HMM:NF012798.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HIR3695622.1"
                     /translation="MTLAFDDELIDANPCRKVRPLPMDDRRTRYLSADEEKALLTALA
                     GQEWLRAVVVAALHTGMRRGELFNLRWFDVDIGRGMIHVRHTKNGRDRTVPINRTVRE
                     VLEHQAKASAYVFPSPKTGARLVDIKRSFKQACTAAALVDFRFHDLRHTAATRLADAG
                     VSVVVIAEVLGHSDIRMTKRYAHAMDEAKREAVERLHHSGVGCQNHAKFQRKEEQPAA
                     RLAVSR"
BASE COUNT          544 a         1147 c          968 g          484 t
ORIGIN      
        1 cgacgtacat gaagaccgac ccgccgttga agttcgcgtg ccgctcgtcg agctgcgaga
       61 aggtgcccga cagatcgtcg gcgaagacct tgtacctcac ccgcacgtgg gtcgtcatgc
      121 caccgagcgt gccgacgcgc cacgtctgcg tgtcgagtct cgtcaccggc aagtccgtca
      181 gtacgcaggt ggcgccgcca cagaagttct ctgcgctgaa ctcctgcacg ttcttcgcga
      241 agtcgaagac cgcgtaacgc cccggtgacc agcgcggcat ctggaagtcc acgtggctcg
      301 cgccgccgac gccgtccacc tcgacccgca cctcgaacag gtgcgtttcg ggacgcggca
      361 tcgacagcgt gtacgcgatg cggtagggcg ccgacgcctg ctcttgcgcc gcacacggca
      421 gcgcgaaagc gaacagcgcg agcaggacga gcgggcggac gagcggacgg acgagggcgc
      481 ggcgcgcgtg tgttgacatg cgacttcgga ctccgtgact tcagaatcga aaactttgtg
      541 gagagacaaa ggacgcgcga ttatccgcgc gcgccgccgc gcgtgtcaaa acccgcgacc
      601 gcccgaaaag gcccgcgcgc actcccggcg gcgacgcctt tcccgcgccc cgccgattga
      661 cgagttgatt gacgatttga ctcgccggcg gatttagctt agaagggcat ctcgacccgg
      721 cgcggccacg ccgcacgttc gtccgccgga aactccttgg ggtttcggac agcggcactc
      781 tcatcatccg tcgagggcga cttatccgac atgggcaaca ccgaacgtct ctactacgac
      841 gacccgcacc tcaccgaatt cgacgctcgc gtcctcgccg tcgagagcgc gtcgggaggg
      901 cgcctcgccg tccgcctcga ccgcaccgcc ttctacccga cgggcggcgg ccagcccacc
      961 gacacgggca cgctcggcgc ggcgcgcgtc gtcgagtgcg tggacgaaga agagggcggc
     1021 gtgctccacc tcgtcgaggg cgaggcgccc gccgcaggcg cgctcgtcaa ttgtcgcgtt
     1081 gactggccgc gccgcctcga acacatccag caccacaccg gccagcacat cctctcgcag
     1141 gcgttcgtcg aactccacgg cgccgagacg cgcagcttcc gcatgacgca cgacgccgcc
     1201 gagatcgacg tggaacttgc ggaaccttcc gacgaaaaga tcgagcgcgc catcgaccgc
     1261 gccaacgaaa tcatctgggc cgaccgcgcc gtgcgcatcc accacgccac gccggacgag
     1321 gccgccgcga tgcccctgcg caaagactcc gcccgcgaag gcgcgctgcg cgtcatcgag
     1381 atcgacaact tcgacttcag cccctgcggc ggcacccacg cgctccgcac gggcgaggtc
     1441 ggcctcgtcg ccgtgcgcca gtgggagcgc gccaaaggtc tcacgcggat caccttcgtc
     1501 gccggcgcgc gcgctctcgc agactaccgc cgcgccaacc acgcggcccg cgccgccgcc
     1561 gccgccttca gcgccgcccg cgacgaagcg cccgcggcgg tcgcgcgtct cctcgaagag
     1621 cacaagcacg cgctgcgccg cctccgcgaa ctctccgccc tcgccgcccg cgtcgaggcc
     1681 cgcgaccttt acgaagaggc cgcgcgccct tcatccgcag cagccccaca cgcctcgacc
     1741 gacgggccgg tcatcgtcgc gcgcgtcttc gaaaaccgcg acgccgaaga gctgaagcaa
     1801 ctcgcgctcg ccctcgccaa acacccccgc gccgtcgcgc tgctcggctc gcgcgacgac
     1861 gccgcgggcg ttcgcgtcgt cttcgcccgc gcccccgaac tcgcgcacga catgaacgcc
     1921 ctcgtgcgcg aggccggcgc ccacctcgaa gggcgcggcg gcgggcgctc cgacctcgcg
     1981 cagggcggcg ggcgccgcgc cgaccgaatc gacgaagtga tcgccgccgc cgccgcccgc
     2041 atctcaagct cccgcgccga acaaaaatga gcgacttcgt gagggaaagg ccgaacttga
     2101 cacttcgcaa aaccgcccgc tattatcgct cgcgcgtggg gcagtagctc agtttggcag
     2161 agcgccgcgt tcgcaatgcg gaggtcaggg gttcgatccc cctctgctcc accaaaagat
     2221 tcaacgactt acagccagcc tcgcggctgg ctgttcttct ttcctctgga atttggcatg
     2281 gttttgacaa cccacgccgg aatgatggag cctctcaacc gcttccctct tagcctcgtc
     2341 catcgcgtgc gcgtagcgtt tcgtcatcct tatgtcggag tgccctagga cctcagcgat
     2401 cacgacgacg ctcacgccgg cgtccgcgag cctcgtagct gctgtgtgcc gcaagtcgtg
     2461 gaagcggaag tccaccaagg ctgcagccgt gcacgcttgc ttgaacgacc gcttgatgtc
     2521 taccagacgc gcgccggtct tcgggctcgg gaatacgtag gcacttgcct tcgcctgatg
     2581 ctctagtacc tcccgtacgg tcctgttgat gggcacagta cgatcccgac cattcttggt
     2641 gtggcggacg tggatcattc ctcgtccgat gtctacgtca aaccaacgca ggttgaacag
     2701 ttccccacgc cgcatgcccg tgtgcagggc cgcgacgacg acggcgcgca gccactcctg
     2761 acctgcaaga gccgtaagca gtgccttctc ctcgtcggcc gaaaggtagc gggtgcgcct
     2821 gtcgtccatg ggcaggggcc gcaccttgcg gcaggggttg gcgtcaatca gctcgtcgtc
     2881 aaaggccagc gtcataaccg ccgacaacac ggcaagttcg cggttgactg tcgccgggtt
     2941 acgcttccaa accaggtcat ttacacaaaa ggcgtgacac tctcggtgcc ggagaaccga
     3001 gcgacagctt cggtatcaag ttctgccgcg ccgcctcata cgcctcccgg tcggcggtgt
     3061 ccggcggcga agggatggtc acctgctcgc cacacggacg gcgtcaccgg ggaagaacgt
     3121 gaagaacctc ctccatgtgg cgg
//
LOCUS       DBNGOH010000454         2332 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2818499, whole genome shotgun sequence.
ACCESSION   DBNGOH010000454 DBNGOH010000000
VERSION     DBNGOH010000454.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2332)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2332)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2332
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2818499"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1372
                     /locus_tag="AC159P_08970"
     CDS             <1..1372
                     /locus_tag="AC159P_08970"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012648566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIR3695623.1"
                     /translation="ILKATTKALIAAAVAVAASVGLIIWQARHGGSAHSANLTAADMS
                     LIAEGQPPQVRAQLASDPARRKEMAQNLRELLALSEEARAHGVADRPEIKRQLELTRE
                     FVLFQTYAQKQREAGQQPAQLAPEEEVKAYLAEPGTEQKFEQLIEDLKKLNPDAPSEI
                     SEAQKQSVKQQWATAQVIARKAVAAQADKDPKTQLQVRLQQARLLAAVYSEELMKKAE
                     PTDQEIEEYFAKNPDMDPKKAREKAEGILKRAKSGEDFEALAKEFSDEPGAKERGGEL
                     PWFGRGQMVKEFEDAAFAMKEGDVSDIVETRFGYHIIKVTGRRQGKKEAPPHVDGPPS
                     PNAKPPAEAETEEQVQARHILIKQSSAPSDNPFAQPLPPREAAKNAILEEKRKKIVEE
                     IVARRKIEVPEEFAVKQPEPPAPQPGSPHGGEGLAPAPMEAPEPAPETKGGDKGGSPK
                     SPGKSK"
     gene            1442..2302
                     /locus_tag="AC159P_08975"
     CDS             1442..2302
                     /locus_tag="AC159P_08975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013457732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3695624.1"
                     /translation="MQFGDYRVEVVPDAEFRLDGGAMFGVVPRTLWSRSFPPDAENRV
                     RLNANCLFVEAGDERVLVETGMGDKWSPKQAAMYGIERRRSLAESVQAITGCAPEEIT
                     VVVNTHLHFDHAGGNTVLDREGRPAPAFPNARYLVSRSELEHAESPHERDRASYLAEN
                     WRPLAESGQLVPQPDDYEVVPGLRMETVPGHSRTMQCPRLERGGRTLYGFADLVPTRA
                     HLPAAWVMGYDLYPVETLEAKKRLLPQAAREGWLCLFYHDPEEPLCRLVEEEGKLRAV
                     GVGEQQSMHT"
BASE COUNT          461 a          814 c          785 g          272 t
ORIGIN      
        1 aattttgaaa gcaacgacta aagcactcat tgccgccgcg gtcgcggtcg ccgcctccgt
       61 cggcctcatc atctggcagg ccaggcacgg cggctcggcg cacagcgcca acctgacggc
      121 ggcggacatg agcctcatcg ccgagggcca gccgccgcaa gttcgcgcgc agctcgccag
      181 cgacccggcg cggcgcaaag agatggcgca gaatttacgc gagctgctcg ccctctccga
      241 agaggcgcgc gcgcacggcg tcgccgaccg ccccgagatc aagcggcagc tcgaactgac
      301 gcgcgagttc gttctcttcc agacctacgc gcagaagcag cgcgaggccg gacagcagcc
      361 cgcccagctc gcacccgaag aagaggtcaa ggcttacctc gccgagccgg gcaccgagca
      421 gaagttcgag cagctcatcg aagacctgaa gaagctcaac ccggacgccc cctcggagat
      481 cagcgaagcg cagaagcaga gcgtcaagca gcagtgggcg acggcgcagg tcatcgcgcg
      541 caaggccgtc gccgcgcagg ccgacaaaga ccccaagacg cagctccagg tgcggctgca
      601 acaggcgcgg ctgctcgccg ccgtctactc cgaagagttg atgaagaagg ccgagccgac
      661 cgaccaagag atcgaagagt atttcgccaa gaaccccgac atggacccga agaaggcgcg
      721 cgagaaggcc gaagggattc tgaagcgcgc caagtccggc gaggacttcg aggcgctcgc
      781 caaggaattc tccgacgagc cgggcgcgaa ggagcgcggc ggcgagctgc cgtggttcgg
      841 gcgcggccag atggtcaagg agttcgagga cgcggccttc gccatgaagg aaggcgacgt
      901 gagcgacatc gtcgagacgc gcttcggcta tcacatcatc aaggtcacgg gccgccggca
      961 gggtaagaag gaggcgccgc ctcacgttga cggcccgccg tcgccgaacg ccaagccgcc
     1021 cgccgaagcc gagaccgaag agcaggtgca ggcccgccac atcctcatca agcagagttc
     1081 ggcgccgagc gacaacccgt tcgcgcagcc cttgccgccg cgcgaggcgg ccaagaacgc
     1141 catcctcgaa gagaagcgca agaagatcgt cgaagagatc gtcgcgcgcc ggaagatcga
     1201 agtccccgaa gagttcgccg tcaagcaacc ggagcctccc gcgccgcaac ccggctcgcc
     1261 gcacggcggc gagggcctcg cgcccgcgcc gatggaagcc cccgaacccg cgcccgaaac
     1321 gaagggcggc gacaagggag gcagtccgaa atcgccgggt aaaagcaagt aaagcgcacg
     1381 ccttgcagcc gcgggcgcgg gtcatcgaac gcccgcgccc gcggcgctga ataattcccc
     1441 aatgcagttc ggcgattatc gcgtggaggt cgtgcccgac gcggagttcc gcctcgacgg
     1501 cggggcgatg ttcggcgtcg tgccgcgcac gctctggtcg cgctcattcc cgcccgacgc
     1561 ggagaaccgc gtgcgcctga acgcgaactg cctgttcgtc gaggcgggcg acgagcgcgt
     1621 cctcgtcgag accgggatgg gcgacaaatg gtctccgaag caagccgcga tgtacggcat
     1681 cgagcgacgg cggagcctcg ccgagtctgt gcaagcgatc accggctgcg cgcccgaaga
     1741 gatcacggtc gttgtcaaca cgcacctgca cttcgaccac gcgggcggca acaccgtgct
     1801 cgaccgggag gggcggcccg cgcccgcttt tcccaacgcg cgctacctcg tctcgcgctc
     1861 cgagcttgag cacgccgaaa gcccgcacga gcgcgaccgc gccagctacc tcgcggagaa
     1921 ctggcgcccg ctcgccgaga gcgggcagct cgtgccgcag ccggacgact acgaggtcgt
     1981 gcccggcctc cgcatggaga ccgtccccgg ccactcgcgc acgatgcagt gcccgcggct
     2041 cgaacgcggg gggcgcacgc tctacggctt cgccgacctc gtgccgacgc gcgcgcacct
     2101 gccggcggcg tgggtcatgg gctacgacct ctacccggtc gagacgctcg aagccaaaaa
     2161 gcgcctgctc ccgcaagccg cgcgcgaggg ctggctctgc ctcttctacc acgacccgga
     2221 ggagccgctc tgccgcctcg tcgaagagga agggaagctg cgcgccgtcg gcgtcggcga
     2281 acagcagtcg atgcacacct gatggcgaga cgagggatgg tcaagacagg cg
//
LOCUS       DBNGOH010000455         3203 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1353148, whole genome shotgun sequence.
ACCESSION   DBNGOH010000455 DBNGOH010000000
VERSION     DBNGOH010000455.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3203)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3203)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3203
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1353148"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(118..381)
                     /locus_tag="AC159P_08980"
     CDS             complement(118..381)
                     /locus_tag="AC159P_08980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695625.1"
                     /translation="MKRSVGVERLELRMRGVSPRAAREAVRGVARELLEELARAGTGG
                     RGGHTHIERVDAAVQSTEAGATGPDLRGAVAREVAAAVRARMK"
     gene            complement(390..2645)
                     /locus_tag="AC159P_08985"
     CDS             complement(390..2645)
                     /locus_tag="AC159P_08985"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003450432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR3695626.1"
                     /translation="MRDGDDEPIRVMKATSKQSGLVTFTPRLGAADDVANYWMRQATL
                     RLRREVCWCWSERGAAAPPQGSAALPPFTDRASVVLDLSRFWEEKQRFYLTDVTARYL
                     TEQLDAPPPRTRGEPARGSFAWVVAELELDDAASFILALALTTAFDASMGSVVAACLN
                     DPARTHPSLALAQKLWDAPEDVLALSDAAHPLFARGIITRGRADTHRYAETDWDAPLT
                     VPSIVARQLLFPDAPLPHSLAPLADAEGIHAQTESARLVALRLGASEAKALRVVPVLG
                     PKGAARRATVAAVARAAGCAAVASASDAARPEDAHQLNALATLCWLRDLYLFLSREPV
                     PHAHGPEAHAAEAALPSTTIPATLFLASDARAQFAHLSAKLLLPAVHVPAFTYADRVA
                     HWKHALGAKGRGLDAVIAEASRRFRFEKETIASVAEGLKKLPRRIAEEDFFAACRAEL
                     DLDIGELASRVAPRFEREQLILPHKQHVQFEEHVRAMRSLTEVHYGWGTARVWNESGI
                     SVLFAGPPGTGKTMAAEILAVKLGLPMYRIDLSQVVNKYIGETEKNLKRLFDAADVSD
                     VILFFDEADALFGKRSEVKDAHDRYANLEISYLLERMERFKGLAILATNRKKDLDEAF
                     LRRLRYVIDFPLPGEREREQMWRQMTPADVDASELDFAFLARQFQLAGGHIRSVIFNA
                     CLQSARREGGAGGPARLSMREVIVAVKREYDTLNRTSSLTQYGPYAPFVEALEQGRGA
                     AAETVETAPRG"
     gene            complement(2647..>3203)
                     /locus_tag="AC159P_08990"
     CDS             complement(2647..>3203)
                     /locus_tag="AC159P_08990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695627.1"
                     /translation="GSDLHLSGLSVMLGTVELPVTMQRPDQLQFKARGDLLTGERISA
                     GGQPLTVVQAISATRRRRSNILVANLLPTLFSVGLLAPASVPLPPPARVRATFALGGL
                     LLGNDEDDVLLALYRDGQTVQVLDYFLKEADIPAPPPPPPVPAQTRKRLVMVTADAVL
                     PGDYRVILRVNGQQAKQSPTVTFV"
BASE COUNT          453 a         1088 c         1155 g          507 t
ORIGIN      
        1 cggcttcaat gtcgaagttg tcgtcgcggc gggctgggac atgcccggcc cctacggttt
       61 ctcaacgcat tcttgttcat tcgccactca tccccgtctt tcattcatcc ccgcgagcta
      121 cttcatgcgc gcgcgaaccg cggcggcgac ctcgcgcgcg accgcgcccc gcagatcggg
      181 gccggtcgcc ccggcctcgg tggattgcac ggcggcgtca acgcgctcga tgtgcgtgtg
      241 cccgccgcgc ccgccggtgc ccgcgcgcgc gagttcttcg agcagctcgc gcgctactcc
      301 gcgaaccgct tcgcgcgccg cgcgcggcga gactccgcgc atgcgcagct ccagacgttc
      361 gacgccgacg cttcgcttca tgctcatcct tacccgcgcg gcgccgtttc gacggtctca
      421 gccgccgcgc ccctgccctg ctccagcgcc tcgacgaacg gggcgtaggg gccgtactgc
      481 gtcaggctcg acgtgcggtt cagcgtgtcg tactcgcgct tgacggcgac gatcacctcg
      541 cgcatcgaga ggcgggccgg gccgcccgcg ccgccttcgc gccgtgccga ctggagacag
      601 gcgttgaaga tgacggagcg gatgtggccg cccgcaagct ggaactgccg cgcgaggaat
      661 gcgaagtcga gttccgaggc gtccacgtcc gcgggcgtca tctgccgcca catctgctcg
      721 cgctcgcgct cgccggggag cgggaagtcg atgacgtagc gcagccgccg caggaaggct
      781 tcgtccaaat ccttcttgcg gttggtggcg aggatggcga ggcccttgaa gcgctccatg
      841 cgttcgagca ggtagctgat ttcgaggttg gcgtagcggt cgtgcgcgtc cttgacttcg
      901 ctgcgcttgc cgaagagcgc gtcggcctcg tcgaagaaga ggatcacgtc cgacacgtcc
      961 gccgcgtcga agagccgttt gaggttcttc tccgtctcgc cgatgtactt gttgaccacc
     1021 tgcgacaaat cgatgcggta catcggcagg ccgagtttga cggcgaggat ttccgcggcc
     1081 atcgtcttgc ccgtgcccgg cggccccgcg aagagcacgg agatgccgct ctcgttccac
     1141 acgcgcgccg tgccccagcc gtaatgaact tcggtgagcg aacgcatcgc gcggacgtgc
     1201 tcctcgaact gcacgtgctg cttgtgcggc aggatcagtt gctcgcgctc gaagcgcggc
     1261 gcgacgcggc tcgccagctc gccgatgtcg aggtcaagct cggcgcggca ggcggcgaag
     1321 aagtcttcct cggcgatgcg ccgcggcagt ttcttcagcc cctcggcgac cgatgcgatg
     1381 gtctccttct cgaagcggaa gcggcgcgac gcttcggcga tgacggcgtc gagtccgcgc
     1441 cctttcgcgc cgagcgcgtg cttccagtgg gcgacgcggt cggcataagt gaacgcgggc
     1501 acatggacgg cgggcagcag gagtttcgcc gaaaggtggg cgaactgcgc gcgcgcgtcc
     1561 gaggcgagaa agagcgtcgc gggaatcgta gtggaaggca acgccgcctc cgccgcgtgt
     1621 gcttccgggc cgtgcgcgtg gggcacaggc tcgcggctga ggaacaggta caggtctctc
     1681 agccagcaga gtgtcgccag cgcgttcagt tgatgcgcgt cttcgggccg cgccgcgtcc
     1741 gacgcggacg cgactgccgc gcaaccggct gcgcgcgcga ccgccgcgac ggtcgcgcgc
     1801 cgcgcggcgc ccttcgggcc gagcacggga acaacgcgca gcgccttcgc ctcggacgcg
     1861 ccgagccgga gcgcgacgag tcgcgccgat tcggtctgcg cgtggatgcc ttcggcgtcg
     1921 gcgagcggcg cgaggctgtg cggcagcggc gcgtccggaa acaacagttg ccgcgcgacg
     1981 atggagggca cggtgagcgg cgcgtcccag tcggtctcgg cgtagcggtg tgtgtccgcg
     2041 cggccacgcg tgatgatgcc gcgcgcgaag agcgggtgcg cggcgtccga gagcgcgagc
     2101 acgtcttctg gcgcgtccca gagcttctgc gcgagcgcca gactggggtg cgtccgcgcc
     2161 gggtcgttga ggcaggcggc gacgacgctg cccatgctgg cgtcgaaggc tgtcgtcagc
     2221 gcgagcgcga ggatgaaaga ggccgcgtcg tcgagttcga gttcggcgac gacccacgcg
     2281 aacgagccgc gcgcgggttc gccccgtgtg cgcggcggcg gcgcgtcgag ttgttcggtc
     2341 aggtagcgcg ccgtcacgtc cgtcaggtag aacctctgct tctcttccca gaagcggctc
     2401 aggtcgagca cgaccgacgc gcggtcggtg aagggcggca gcgccgcgct gccctgcggc
     2461 ggggccgcgg cgccgcgctc cgaccagcac caacagacct cgcgccgcag tcgcagcgtg
     2521 gcctgccgca tccagtagtt ggcaacgtcg tccgccgcgc cgaggcgcgg cgtgaacgtg
     2581 accagcccgc tctgtttact cgttgccttc atcacacgaa tcggctcgtc gtcgccgtct
     2641 cgcacgtcac acgaaggtga cggtcggact ctgtttggcc tgctggccgt tgacgcgcag
     2701 gatgacgcgg tagtcgccgg gcagcaccgc gtccgccgtg accatcacga ggcgcttgcg
     2761 cgtttgcgcg gggacgggcg gcggcggggg cggcgcgggg atgtccgcct ccttcaagaa
     2821 gtagtccaac acctgcacgg tctggccgtc gcggtagagc gcgagtaaga catcgtcctc
     2881 gtcgttgccg aggagcagcc ctccgagcgc gaacgtcgcg cgcacgcgcg cgggcggcgg
     2941 cagaggcacg gacgcgggcg cgagcagccc cacgctgaac agcgtgggga gcaggttggc
     3001 gacgaggatg ttgctccgcc tgcgccgcgt cgcactgatt gcctgcacga ccgtcaaggg
     3061 ctggccgccc gcggagatgc gctcgcccgt cagcaaatcg ccgcgcgctt tgaactgaag
     3121 ctggtcgggc cgctgcatcg tcaccggaag ctcgaccgtg ccgagcatga cggagaggcc
     3181 gctgaggtgc aggtctgagc cgc
//
LOCUS       DBNGOH010000456         1571 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_69598, whole genome shotgun sequence.
ACCESSION   DBNGOH010000456 DBNGOH010000000
VERSION     DBNGOH010000456.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1571)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1571)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1571
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_69598"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..551
                     /locus_tag="AC159P_08995"
     CDS             <1..551
                     /locus_tag="AC159P_08995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695628.1"
                     /translation="FVGAFNGNRFFTDNNRQLNYFARVRKIFDRRFAVGMSAQLGKQL
                     LPPGASGSNNENIFGVDFQYAVTSRLGLRGEFVEGNTPSTLLGIEPEFAPAFRPGSHS
                     AGGHLFAGYRLTDRDNLYARYDQFNGDPVTGRSVRAFNFGFFRQVGGQSRLSFDYQLK
                     NRPSFNDDAVNGRLNLTWGIEF"
     gene            564..974
                     /locus_tag="AC159P_09000"
     CDS             564..974
                     /locus_tag="AC159P_09000"
                     /inference="COORDINATES: protein motif:HMM:NF024864.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HIR3695629.1"
                     /translation="MASRKLISASAAALLAVSLSFAAACGAQQGGAATSVQSNSTAQG
                     AGQRVEISASKYEFKPRELKLKAGRPVTVVLTSTGGSQSFDVDELNLHSAEAAEGKTI
                     TYEFTPDKPGTDEFYCAHGGHKERGMTGRLEIAP"
     gene            1006..>1571
                     /locus_tag="AC159P_09005"
     CDS             1006..>1571
                     /locus_tag="AC159P_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007220500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HIR3695630.1"
                     /translation="MKRARRRRILYLVSSCLILLSAACGGRQASGPAEAGGTDNAGRE
                     VTVYVSTDRVFSEPVLRAYEQRTGVKVNAVYDTEETKSTGLANKLLAEKNRPQADVFW
                     SNEPVRTLVLKKNGVLAPYKSPSAEGIPATFKDPEGYWTGFSARSRVIVYNTNLVKAE
                     EAPMSVFDLADPRWKEQVAVADPRFGSTS"
BASE COUNT          302 a          508 c          500 g          261 t
ORIGIN      
        1 actttgtggg cgcgttcaac ggcaaccgtt tcttcacgga caacaaccgc cagctcaact
       61 acttcgcccg cgtgcggaag atcttcgacc gccgcttcgc cgtcggcatg tcggcgcagc
      121 taggcaagca gcttctgccg cccggcgcgt cgggcagtaa caacgaaaac atcttcggcg
      181 tagatttcca gtacgccgtg acgagccgcc tcggcctgcg cggcgagttt gtcgagggca
      241 acacgccctc gacgctactc ggcatcgagc cggagttcgc ccccgccttc cgtcccggct
      301 cgcactctgc gggcgggcac ctgttcgcgg gctacagact gacggacagg gacaacctat
      361 atgcgcgcta cgaccagttc aacggagacc ccgtgacggg gcgcagcgtc cgcgcgttca
      421 actttggctt cttccggcag gtcggcgggc agtcacgcct cagcttcgac taccagctca
      481 agaaccgccc gtcgttcaac gacgacgccg tcaacgggag actgaacctc acctggggca
      541 tcgaatttta gaggaggttc gacatggctt ctcgaaaact aatatcggcg tcggccgccg
      601 ctttactcgc cgtcagtctc tccttcgccg cggcctgcgg cgcgcaacag ggcggcgccg
      661 ccacgagcgt ccagtcaaac tctacggcgc agggcgcggg gcagcgtgtt gagatcagcg
      721 cgagcaaata cgagttcaag ccgcgcgagc tgaagctcaa ggcgggacgg cccgtcacgg
      781 tcgtgctcac cagtacgggc ggcagccagt ctttcgacgt tgacgagctg aacctccaca
      841 gcgcggaagc ggcggaaggc aagacgatca cctacgagtt cacgcccgac aagccgggca
      901 ctgacgagtt ctactgcgcc cacggcggcc acaaagagag gggcatgacg ggccgtttag
      961 agatcgcgcc ctgaggatgg aaaacagttg ggggtggaac aaactatgaa acgcgcacgt
     1021 cgccgtcgaa ttctttacct cgtctcttct tgtctcatac tgctctcggc agcgtgcggt
     1081 ggtcggcagg cgtccggccc ggcggaggcc ggcggcacgg acaacgcggg gcgggaggtc
     1141 acggtctacg tctcgacgga tcgcgtgttc tcggaacccg tcctgcgcgc ttacgagcaa
     1201 cggacgggcg tgaaagtcaa cgccgtctac gacacggagg agaccaagtc cacggggctc
     1261 gccaacaagc tgctcgccga gaagaaccgc ccgcaggccg acgtgttctg gtcgaacgag
     1321 ccggtacgta cgctcgtgct gaagaagaac ggcgtgctcg cgccgtacaa gtcgccgagc
     1381 gccgaaggca tcccggccac gttcaaagac cccgaaggct attggacggg tttctcggcg
     1441 cgctcgcgcg tgatcgtcta caacacgaat ctggtgaagg cggaggaagc tccgatgtct
     1501 gtgttcgatc tcgccgaccc gaggtggaag gagcaggtgg cggtcgccga cccgcgcttc
     1561 ggctctacct c
//
LOCUS       DBNGOH010000457         2105 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_69616, whole genome shotgun sequence.
ACCESSION   DBNGOH010000457 DBNGOH010000000
VERSION     DBNGOH010000457.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2105)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2105)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2105
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_69616"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..197)
                     /locus_tag="AC159P_09010"
     CDS             complement(<1..197)
                     /locus_tag="AC159P_09010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695631.1"
                     /translation="MKFNHTTFFNGYRDLYGKLNQTQVSGLDNLLGYIELDNDVADVR
                     WAAYMLATIKHECADTFQPIT"
     gene            complement(394..765)
                     /locus_tag="AC159P_09015"
     CDS             complement(394..765)
                     /locus_tag="AC159P_09015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR3695632.1"
                     /translation="MGQQNVTWHRWEEMPRERVNQTLERRLITGDRIMLTHVYLEKGS
                     VVPRHAHENEQITYVLEGALRFWIGDEGAQVFDVRAGEVLCIPSNVPHKAEALEDTLD
                     VDIFSPPRQDWLDKTDDYLRR"
     gene            821..1501
                     /locus_tag="AC159P_09020"
     CDS             821..1501
                     /locus_tag="AC159P_09020"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HIR3695633.1"
                     /translation="MTRTRAGVPRVKICCIGSLEEAALAVRHGAAALGLVSAMPSGPG
                     VIGEDLIARIAARVPPGVASFLLTCEQDAGRIIAQQRRTRVGTIQICDHLAHDAYAEL
                     RRAMPGVALVQVVHVGGEESVAEAAAVAPHVDAILLDSGNRQLAVKELGGTGRTHDWE
                     VSRRVREAVNVPVYLAGGLKAENVAGAVARVAPFGVDVCSGVRTDGKLDEAKLAAFFR
                     AVAQGAAA"
     gene            1535..1783
                     /locus_tag="AC159P_09025"
     CDS             1535..1783
                     /locus_tag="AC159P_09025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695634.1"
                     /translation="MAAHLDADAAEPTVARLVRRVVTDDVTVVDVGEFDTDVHLPRGG
                     CEPSLARIENPAVRFRSLLQKIQTKAIRTQREHNMRRR"
     gene            1887..>2105
                     /locus_tag="AC159P_09030"
     CDS             1887..>2105
                     /locus_tag="AC159P_09030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695635.1"
                     /translation="MRRVRTFVNVVVAVSLSLLALPLADGGARAQAGGPTILVPNLGV
                     RAVAENLVTPTSVAFLGPGDMLVLEKDTG"
BASE COUNT          344 a          643 c          729 g          389 t
ORIGIN      
        1 tcggtgatgg gctggaaggt gtcggcgcac tcgtgcttga tggtggcgag catgtaggca
       61 gcccagcgca cgtcggcgac atcgttgtcg agttcgatgt agccgagcag gttgtcgagg
      121 cccgagacct gcgtctggtt gagcttgccg tacagatcgc ggtagccgtt gaagaacgtc
      181 gtgtggttga atttcatgaa cgtctcctga agaagcgtga gccaggggtc gaagagcttt
      241 cggaacttgc gagccagcaa catacatcac gggcgcgcgc gatttcaacg ttcagcaggc
      301 agcaggcagt aaagaagcct tgaatatgaa atccgaaagt tgaaatcaga aatcgtattg
      361 actgcccggt gcctgctgcc tgctgcccgc tgcctaccgt ctcaggtaat cgtccgtctt
      421 gtcgagccag tcctggcggg gcgggctgaa gatgtccacg tcgagcgtgt cttcgagcgc
      481 ctcggccttg tgcgggacat tggaagggat gcagagcact tcgccggcgc gcacgtcgaa
      541 aacttgggcg ccttcgtcgc cgatccagaa gcggagcgcg ccttcgagga cgtaggtgat
      601 ctgctcgttc tcgtgcgcgt ggcgcgggac gacggagcct ttttcgaggt agacgtgcgt
      661 gagcatgatg cggtcgccgg taatcagccg ccgctccagc gtctggttga cgcgctcgcg
      721 cggcatctcc tcccagcggt gccaggtgac attctgttgc cccatgcatc ctcctcctga
      781 actatattag tgtgacttcg agagcgacgg gccgaaactt atgacacgaa cgcgcgcggg
      841 cgtgccgaga gtcaagattt gctgcatcgg gagtttggag gaggccgcgc tcgccgtccg
      901 ccacggcgcc gcggcgctcg gcctcgtctc cgcgatgccg agcggccccg gtgtcatcgg
      961 cgaagacctg atcgcgcgca tcgccgcgcg cgtgccgccc ggcgtcgcct cgttcctcct
     1021 cacctgcgaa caggacgcgg ggcgcattat cgcacagcag cggcgcacgc gcgtgggcac
     1081 gattcagatt tgcgaccatc tcgcgcacga cgcctacgcg gagctgcgcc gcgcgatgcc
     1141 gggcgtcgcg ctcgtgcagg tcgtgcacgt cggcggcgag gagtccgtcg cggaggccgc
     1201 cgcggtcgcg ccgcacgtgg acgcgatcct gctcgactcg ggcaaccggc agctcgcggt
     1261 gaaggaactc ggcgggacgg gccgcacgca cgattgggag gtgagccggc gcgtgcgcga
     1321 agcggtgaac gtgccggtct acctcgcggg cgggttgaag gcggagaacg tcgccggggc
     1381 cgtggcccgc gtcgcgccgt tcggcgtgga cgtgtgcagc ggcgtacgga cggacgggaa
     1441 gctcgacgag gcgaagctcg cggctttctt ccgcgccgtc gctcagggcg cggcggcgta
     1501 gtagctcgtg cggccctgca cttttgtatt cggggtggcc gcgcacctcg acgcggacgc
     1561 ggcggagccg accgtcgcgc gccttgttcg tcgggtagta actgatgacg taacggtggt
     1621 tgatgtcggc gagttcgaca ccgacgttca tctccctcgc ggcggttgcg aaccgagcct
     1681 tgcgcggatc gaaaatccgg cggtgcggtt ccgttcgttg ttgcaaaaaa tccagacaaa
     1741 agcaatccgg acacaacgcg aacacaacat gcggcggcgg tgattttaga ataattccgc
     1801 cgccctcgcc cgcgttttaa gacgcggcgt tcctcatcag gctccccgcc ggttttcccc
     1861 ctgtcaacaa acaaggagta ttcacgatga gaagagtccg tacgtttgtc aacgtcgtgg
     1921 tcgccgtgtc tctgtccctg ctggcgctgc ccctcgcgga cgggggggcg cgggcgcagg
     1981 cgggcggccc cacgatactc gttcccaacc tgggggtgcg tgcggtggcg gaaaatctgg
     2041 tgacgccgac ctccgtcgcc ttcctcggcc ccggcgacat gctcgtgctg gagaaggaca
     2101 cgggg
//
LOCUS       DBNGOH010000458         1824 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3735271, whole genome shotgun sequence.
ACCESSION   DBNGOH010000458 DBNGOH010000000
VERSION     DBNGOH010000458.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1824)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1824)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1824
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3735271"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1014
                     /locus_tag="AC159P_09035"
     CDS             <1..1014
                     /locus_tag="AC159P_09035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695636.1"
                     /translation="LLALGGRLAFDGGKYAGTPLADLLPLALDGRAAPPTAKPEPVFK
                     PALTARGAAHPVTRLNDDRAASQKIWNDLPPVSVPEALGVLKPGATLLAEARRVSGAG
                     APLPLLAHQRYGRGHALAFTASDTWRWQLKMDSKSNAHENFWRQMLRYLVSISPAQVE
                     VAAEQDVYGLGDPVRVVADVRDKKFIPSADARVTARVVKPSGASVEVPLRFTARDETN
                     VYVGDYKADELGRHRIELEAAGTKMGTMSAQSEFLVAELNREFYDAAQNEDLLRRIAA
                     ETGGKFYTLEQTDELINDLTYRKTDNSRLVTKDLWDMPINFLLLVGLFSAEWFLRKRE
                     GLA"
     gene            1128..>1824
                     /locus_tag="AC159P_09040"
     CDS             1128..>1824
                     /locus_tag="AC159P_09040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695637.1"
                     /translation="MTFVACAQAAAQGGNAALVATPNGPTATAAARQPADPDKFAVIL
                     VGASGEEVYAKQFAQWAASLRSALVERLGFGEGNLKVLTEDSADKGAARATAEEVRRT
                     FESLRGAVKEESTVFVFFIGHGSFDGKQAKFNLLGPDLHAGEYRTLLGALAARRVVVV
                     QMASASGEFVKALAGRGRVVVTATRSGQEQNAPRFAEHFIKALTEREADADQNGRVSV
                     LEAFDYAARLVAEH"
BASE COUNT          306 a          617 c          642 g          259 t
ORIGIN      
        1 ctgctggcgc tcggcgggcg gctcgccttc gacggcggca agtacgcggg cacgcctctg
       61 gccgacctgc tgccgctcgc gctcgacggt cgcgccgcgc cgccgaccgc caagcccgaa
      121 cccgtcttca agcccgcgct caccgcgcgc ggcgccgcgc acccggtcac gcgtctgaac
      181 gacgaccgcg ccgcgagcca gaaaatctgg aacgatttgc cgcccgtcag cgtgccggaa
      241 gcgctcggcg tcctgaagcc gggcgcgacc ctgctcgccg aagcgcggcg cgtgtcgggg
      301 gcgggcgcgc cgctgccgct gctcgcgcac cagcgctacg ggcgcggcca cgcgctcgcc
      361 ttcacggcct ccgacacctg gcgctggcag ctcaagatgg attccaagag caacgcgcac
      421 gagaacttct ggcgtcagat gttacgttat ctcgtgagca tttctcccgc gcaggtcgaa
      481 gtcgccgccg agcaggacgt gtacggactc ggcgaccctg tccgcgtcgt cgccgacgtg
      541 agggacaaga aattcatccc ttcggcggac gcgcgagtca cggcgcgcgt cgtcaagccc
      601 tcgggcgcga gcgtcgaggt gccgctccgc ttcacggcgc gcgacgagac gaacgtctac
      661 gtcggcgact acaaggccga cgagctgggc cgccaccgca tcgagctgga ggcggcgggt
      721 acgaaaatgg ggacgatgtc cgcgcagtcg gaattcctcg tcgccgagct gaaccgcgag
      781 ttctacgacg cggcgcagaa cgaagacctg ctccgccgca tcgccgccga gaccggcggc
      841 aagttctaca cgctcgaaca gacggacgag ctgatcaacg acctgaccta caggaagacg
      901 gacaactcgc ggctcgtgac caaagacctg tgggacatgc cgatcaactt cctcctgctc
      961 gtcgggctgt tcagcgccga gtggttcctg cgcaagcgcg aggggctggc ctgacgggcg
     1021 gggaaagaat cggcgggccg gacgttcaga atcgtaccgc tattcggcgt ttgtctacga
     1081 agagcatgat gcgtcgtttc atcagagcag cgatcctggg aggtatcttg acgttcgtcg
     1141 cctgcgcgca ggcggcggcg caaggcggga acgccgcgct ggtcgcgacg ccgaacgggc
     1201 cgacggccac cgccgccgcg cgtcagcccg cagacccgga caagttcgcc gtcatcctcg
     1261 tcggcgcgag cggcgaggag gtttacgcca aacagttcgc gcagtgggcg gcgagtttga
     1321 gaagcgcgct ggtcgagcgt ctcggcttcg gcgaaggcaa cctgaaagtt ctgacagaag
     1381 actccgcgga caaaggcgcg gcgcgcgcga ccgccgagga ggtgcgccgc acgttcgagt
     1441 cgctgcgcgg cgcggtgaag gaagagagca cggtcttcgt cttcttcatc ggccacgggt
     1501 cgttcgacgg caagcaggcg aagttcaacc tgttggggcc ggacttgcac gcgggcgagt
     1561 acaggacgct gctcggcgcg ctggcggcgc ggcgcgtggt cgtcgtccag atggcgagcg
     1621 cgagcggcga gttcgtgaag gcgctcgccg ggcgcgggcg cgtcgtcgtc accgcgacgc
     1681 gcagcgggca ggagcagaac gcgccgcgct tcgccgagca cttcatcaag gcgctcacgg
     1741 agcgcgaggc ggacgccgac cagaacgggc gcgtctcggt cctcgaagct ttcgactacg
     1801 ccgcgcgcct cgtcgccgaa cact
//
LOCUS       DBNGOH010000459         8475 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_987066, whole genome shotgun sequence.
ACCESSION   DBNGOH010000459 DBNGOH010000000
VERSION     DBNGOH010000459.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8475)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8475)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8475
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_987066"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(125..586)
                     /locus_tag="AC159P_09045"
     CDS             complement(125..586)
                     /locus_tag="AC159P_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010940934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR3695638.1"
                     /translation="MKILLATDGSACSDAAVREVARRPWPADTQVRVISVVELPAPLV
                     ADPYMIDADYFEQAERVKRDTATAALERAAATLDESIGGLQVSTEAPTGSPRRVIVEE
                     AEAWGADLIVVGSHGYRTWERMLLGSVSQTVAAHAECSVEIVRCRKEKSGV"
     gene            834..1022
                     /locus_tag="AC159P_09050"
     CDS             834..1022
                     /locus_tag="AC159P_09050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695639.1"
                     /translation="MSKTFHCAVCRKATPTLHMRDLDGKWVGAHCIPERELDACPGLR
                     ERLGLKPARTAQARRKAA"
     gene            complement(1186..1431)
                     /locus_tag="AC159P_09055"
     CDS             complement(1186..1431)
                     /locus_tag="AC159P_09055"
                     /inference="COORDINATES: protein motif:HMM:NF024881.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HIR3695640.1"
                     /translation="MDCDKCLDVLSEFQAGGLGENDSVFIRTHLEICVGCLDVFKDLD
                     SIVKTALLMREENGNGIAYPDESALWVRLSIDKGIVH"
     gene            complement(1485..2018)
                     /locus_tag="AC159P_09060"
     CDS             complement(1485..2018)
                     /locus_tag="AC159P_09060"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR3695641.1"
                     /translation="MAQKSAAGDSQAFGELYHRHFRRVYALTLRMMGNPTEAEDMTQE
                     VFLQLFNKIGSFRGESAFTTWLHRMTVNQVLMHFRKRSTRTELLTDEGETPVQIVRGT
                     ENPDAMPVIDRISLERAMAQLPTGYRTVFVLHDVEGYEHYEVAEMLGIAEGTSKSQLH
                     KARLKLRQLIRQQSASK"
     gene            complement(2283..4007)
                     /gene="rpoD"
                     /locus_tag="AC159P_09065"
     CDS             complement(2283..4007)
                     /gene="rpoD"
                     /locus_tag="AC159P_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011525093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HIR3695642.1"
                     /translation="MEEKDQDDVMQRLLELGGDKKFLSFDDLNRELPDNVVSPDDIED
                     VLQKLEGSNITVADSDERLIEQAAASPSSDDLDAELDEELELDLSAGALEKTNDPVRL
                     YLREMGVVPLLTREGEVSIAKRIERGQIKTQKAISRSPIAVKELCKVGDELTASRLNI
                     RDIVNFSEQAELTGQEDKADEYLRWTIEGVENIRRSFKNGIKEWEKLRAEQKLLRGKK
                     SKKVLRLKRKVARMRLEISQEIKLLNMTEKARQRLIDSIRKVAKEIRVAEREIENNTE
                     KLNRKRIRPDEEKEFKRLIAAARKRLQQIEADHNVSPVEIKRSLQTIVTGETETATAK
                     RELIEANLRLVVSIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEWRRGYKFSTYATWW
                     IRQAITRAIADQARTIRIPVHMIETINKLIRTSRALVQELGREPTSEEIARKMDIPVS
                     KVRKVLKIAQEPISLETPIGEEEDSHLGDFIEDKSRVSPADSVVTTNLRQVTDEVLAT
                     LSPREEVVIKLRYGLGPQGEEHTLEEVGKNFDVTRERIRQIEAKALRKLRHPSRARLL
                     KPFNEGLQ"
     gene            complement(4277..6109)
                     /gene="dnaG"
                     /locus_tag="AC159P_09070"
     CDS             complement(4277..6109)
                     /gene="dnaG"
                     /locus_tag="AC159P_09070"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HIR3695643.1"
                     /translation="MRFPQVFIDDLRRQADIVRVISDYVTLKKKGANWMACCPFHQEK
                     SPSFSVSPSKDIFYCFGCAKGGSVFNFVMELERVSFPEAVRIVAEKTGVPLPVMVDDK
                     GYESRRKEADEVVQLNGWALEFWERQLAEGQEAQAAREYVEGRGISEETQKVFRMGYA
                     PDSWDALGNYLKSKGASVAQIERSGLVVKKDAGGFYDRFRGRVVFPVMDAQGRPVAFG
                     ARAIRAGDEPKYLNSPETAAYTKGRHLFGLNVSREEIRKKKFVILVEGYLDLIVPYQF
                     GVRNVVASLGTALTPEQVRLLGRFARKVVVNYDGDKAGVKAAKRAIETLLAEDFEIKV
                     LVLPEGADPDEFLRAHGAEEYNRRRGEAFPYINFVIDQALEGRDLRVPAHKAEAVEEV
                     LPYLRAVRNRIQKREYFDMAMGALRVEEKSLRQELWKAVSAAGASAPAPSDVKQKVAR
                     ASAGHPTLAEQRLLELLVHDAELRGAILPQLEAEDYESLPTAVVFRALKTLHERGDAV
                     DFTTLGELTEGDAVAADVVPLVLMTEHGRAEGEAADSFLVEAESCLVALRLMRIDRRV
                     KELGAEIAAADRAGDAERRDRLVMEDLELKRHRTALLPRAATTQ"
     gene            complement(6127..6519)
                     /locus_tag="AC159P_09075"
     CDS             complement(6127..6519)
                     /locus_tag="AC159P_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017594918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695644.1"
                     /translation="MTDEEQAVMRGDETVMLFRPVGPKELELIRASDWREFPPRLPEQ
                     PIFYPVLNEEYATQIARDWNARRNAGGRGFVTRFRVRRAFLERYDVHTVGGSVHQEYW
                     VPAEELPEFNRNLVGPIEVVAEFSAPTE"
     gene            complement(6519..>8475)
                     /locus_tag="AC159P_09080"
     CDS             complement(6519..>8475)
                     /locus_tag="AC159P_09080"
                     /inference="COORDINATES: protein motif:HMM:TIGR01069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0045910 - negative regulation of DNA
                     recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HIR3695645.1"
                     /translation="SEEAIQDQLVTVRNDRFVIPVRSDHRGRVHGVAHGFSSSGATVF
                     VEPLETIESNNELQALRETEEREITRILFTLTEELRAEIGSIEEAARAVAELDFVGAK
                     ATLSNHLRCTEPAISDDETLELEEARHPLLEENLRAQGREVVPVSLSLDAARPVMVIS
                     GANAGGKTVVLKTAGLSSLMALSGLHVAARAARVPLYASVLADIGDRQSLAANLSTFT
                     SHVANIRRMIELCERPALVLLDEVGTGTDPEEGSALGVAVVDHFRRACGAHVVATTHY
                     SGLKMYAANEEGVQNASVEFDEKTLQPTYRLLVGLAGSSAGLDIAQRFGIPARVIGAA
                     REFVDEKSLAAAEYLRRIKQESEEAEGLRRALEEERRAVADKYAALDRDAERRERERQ
                     ASFARELERAIADFELRARELYSKIEDRAERVRVEREAERRAAELRREAQAQRTQARA
                     ATASPQTRETGSGVRVRRPGARADEGDAVPSSDALAVPQREIVVGDRVRLRTLGSEGV
                     VERVSGSEAEVRVKHLRFREKVRNLELVAPAPEQKDDSLAARLRDMQKRGTEVHLSKQ
                     AREAPDAELNLIGKTTDEAADETDKFLDDAYLHGLAHVRIIPGHGTGALRRAVAELLR
                     THPHVARFAAAPDNQGGAGATVVELKQ"
BASE COUNT         1247 a         2794 c         2815 g         1619 t
ORIGIN      
        1 tcacaaatcc tctcagtccg aagtctggaa tctggagtct ggagtctgga gtcagaacag
       61 aagccatgct gaattcgact ccgcgcgcgg tatagctttc ggctcagact ccggcctcca
      121 gacttcagac tccagacttc tctttcctgc acctgacgat ttcgaccgag cattcggcgt
      181 gggcggcgac cgtctgcgag accgagccga gcagcatgcg ctcccaggtg cggtagccgt
      241 gcgagccgac gacgatgaga tcggcgcccc acgcttcggc ttcttcgacg atgacgcggc
      301 gaggcgagcc ggtcggcgct tcggtcgaga cttgcaggcc gccgatgctc tcgtcgagcg
      361 tcgccgcggc gcgttcgagt gcagccgtgg ccgtgtcgcg tttcacgcgc tcggcctgtt
      421 cgaagtagtc ggcgtcgatc atgtacgggt cggcaaccag cggcgccggc agctcgacga
      481 cagagatgac gcgcacctgc gtgtccgcgg gccacggcct gcgcgcgacc tctctgaccg
      541 ccgcgtcgct gcatgccgag ccgtccgtcg ctaaaagtat cttcatcgcg ttgctccttc
      601 ggaagggact tcagggcgtc ctccgcagtc cgtatgggca acgtgtgtgc cgaacttttt
      661 cgggccgatt cgtgcgacgg ctcgatgtgc gccggggaaa tctcaccagc gcggcgtaat
      721 ttttcgcgat gtttcacccg tgcaactttg ttgcacagcc gccgcgccgc ggttataatg
      781 gcgtgtggcc gcgacgagcg cgcggcctaa ttctcggcgg ggggtttgta gaggtgagta
      841 agacttttca ctgcgcggtc tgccgcaagg cgaccccgac gcttcacatg cgcgatctgg
      901 acggcaagtg ggtcggcgcc cactgcatcc ccgaaaggga gttggacgcc tgccccggcc
      961 tgcgcgagcg gctcgggctg aagcccgcgc gcacggcgca ggcgcgacgc aaggcggcct
     1021 gaccgttcgg cagcgccggg actcggctcg gctgtcggcg ggccgcaaag agaaaagcgc
     1081 cgccccggag agggagcggc gcttctctcg aatgcctcgg tgattagata ctacgacggc
     1141 ggcgcgcggg gagccggcgc gggcgcgttc gtcgaagggg gccgttcagt gaacgatgcc
     1201 tttgtcgatg ctcaggcgca cccacagcgc gctctcgtcc gggtaggcga tgccgttgcc
     1261 gttctcttca cgcatgagga gcgcggtctt cacaatcgag tcgaggtctt tgaagacatc
     1321 cagacagccg acgcagattt ccagatgtgt ccggatgaag accgaatcgt tctcgccaag
     1381 cccgccggcc tggaactcgc tgagcacgtc gagacacttg tcgcaatcca taggggcttt
     1441 gctcccttag caggtcttag tcttccggcg cgggcgcgtc ggagtcattt agatgcggac
     1501 tgctgccgaa ttaattgcct gagcttcagg cgcgctttgt ggagctgcga tttcgacgtg
     1561 ccctcggcga tgccgagcat ctctgcgacc tcgtaatgct cgtagccttc cacgtcgtgc
     1621 aagacgaaga ccgtgcggta gccggtgggc agttgcgcca tcgcgcgttc gagcgagatg
     1681 cggtcgatga cgggcatcgc gtccgggttc tccgtgccgc ggacgatctg gacgggcgtc
     1741 tcgccctcgt cggtcagaag ttccgtgcgc gtgctgcgct tgcggaagtg catcagcacc
     1801 tgattgaccg tcatccggtg gagccacgtc gtgaacgccg actcgccgcg gaaactgccg
     1861 atcttgttga agagctggag gaagacttcc tgcgtcatgt cctccgcctc tgtggggttg
     1921 cccatcatgc gcagtgtcag cgcgtagacg cggcggaagt ggcggtggta caactcgccg
     1981 aacgcctgcg agtcgcccgc cgcggacttc tgggccagtt cgaaatctga cggctgtttc
     2041 ttttgctcga cctcggcaac gctcacgatt cgcgcatccc cttctgccgg aatttatcgt
     2101 ttcggcgaga ttaacattaa tccgaccgaa ttcaaaaaac gggggaggtg gagccgaaca
     2161 ctgactggcg gggaatcggg cacggcgggg gtttgaatgg gggcgggatt caaaccggcg
     2221 aatcccgccc ttctgttcgt tcggcggacg gcgcgaagtt gcggcgcgtc cggcgtcgcg
     2281 gcttactgca agccttcgtt gaagggcttg agcaggcgcg cgcgcgaggg gtggcggagc
     2341 ttgcggagcg ccttggcctc gatctggcgg atgcgctcgc gcgtcacgtc gaagttcttg
     2401 ccgacctctt cgagcgtgtg ctcctcgccc tgcgggccga ggccgtagcg cagcttgatg
     2461 acgacctctt cgcgcgggct gagggtcgcc agcacctcgt ccgtcacctg ccgcagattc
     2521 gtcgtcacga ccgagtccgc cggactcaca cgcgacttgt cctcgatgaa gtcgccgagg
     2581 tgcgagtctt cctcttcgcc aatcggcgtt tccagagaga ttggctcctg cgcgatcttc
     2641 aaaactttgc gcaccttcga gacggggatg tccatcttgc gcgcgatctc ctcgctcgtc
     2701 ggctcgcggc caagctcctg cacgagggcg cgcgaggtgc ggatcagctt gttgatcgtc
     2761 tcgatcatgt ggacggggat gcggatcgtg cgcgcctgat cggcgatggc ccgcgtgatg
     2821 gcttgccgaa tccaccacgt ggcgtaggtc gagaacttgt agccgcggcg ccactcgaac
     2881 ttatcgaccg ccttcatcag gccgatgttg ccctcctgaa tcaggtcgag gaactgcaac
     2941 ccgcggttgg tgtacttctt ggcgatggag acgacgaggc gcaggttggc ttcgatcaac
     3001 tcgcgcttgg cggtggcggt ctcggtctcg ccggtgacga tggtctggag cgagcgcttg
     3061 atctcgacgg gcgagacgtt gtggtcggcc tcgatctgct ggaggcgctt gcgcgcggcg
     3121 gcgatcagac gcttgaactc cttctcctcg tcggggcgga tgcgcttgcg gttgagcttc
     3181 tcggtgttgt tctcgatctc gcgctcggcc acgcgaatct ctttcgccac cttgcggatc
     3241 gagtcgatga ggcgctgccg cgccttctcc gtcatgttca ggagcttgat ctcctgcgag
     3301 atttcgaggc gcatccgcgc caccttgcgt ttgaggcgca agaccttctt cgacttcttc
     3361 ccgcgcaaga gcttctgctc ggcgcgcagc ttctcccatt ccttgatgcc gttcttgaag
     3421 ctgcggcgga tgttctcgac gccctcgatg gtccagcgca ggtactcgtc ggccttgtcc
     3481 tcctgccccg tcagctcggc ctgctcggag aagttgacga tgtcgcggat gttgaggcgc
     3541 gaggccgtca gctcgtcgcc caccttgcac agctccttga cggcgatggg cgagcgcgag
     3601 atggccttct gcgtcttgat ctgcccgcgc tcgatgcgct tggcgatgga gacctcgccc
     3661 tcgcgcgtca gcagcgggac gacgcccatc tcgcgcaggt acaggcggac ggggtcgttc
     3721 gtcttctcca acgcgcccgc cgagaggtcg agttcgagtt cttcgtcgag ttcggcgtcg
     3781 aggtcgtccg aagagggcga ggcggcggcc tgctcgatga ggcgctcgtc ggagtcggcg
     3841 accgtgatgt tcgagccttc gagcttttgc agcacgtcct cgatgtcgtc gggcgagacg
     3901 acgttgtcgg gcagctcgcg gttgaggtcg tcgaacgaga ggaacttctt gtcgccgccg
     3961 agctccagca ggcgctgcat cacgtcgtcc tgatctttct cttccatgct cacgtcttcg
     4021 tccacactct cggctccgca aatccttggg gaacgccgcg ctctcggtgg ggccgcggcg
     4081 gggtgaaagt ttaatcgaac cgggcgccgc ccgtcacgcc ctccgagagt aggcagtagg
     4141 cagcaggcag taggcagtca agacaaaaac agatttcgaa tctcagattt gagatttcaa
     4201 acttctgatt caaatattca cgttgcaaat tcaaatcccg gttgttcttg actgcctact
     4261 gcctactgcc tactgcctac tgtgttgttg cggcgcgcgg caggagcgcc gtgcggtgtc
     4321 gcttcagttc gaggtcttcc atgacgagcc ggtcgcgccg ctccgcgtcg cccgcgcggt
     4381 cggcggcggc gatctcggcg ccgagttcct tgacgcggcg gtcgatgcgc atcagccgca
     4441 gcgcgactaa gcagctctcg gcctcgacga ggaaggagtc ggcggcctcg ccctcggcgc
     4501 gcccgtgctc ggtcatcagt acgagcggca ccacgtcagc cgccaccgcg tcgccctcgg
     4561 tcaactcgcc gagcgtcgta aaatcgaccg cgtcgccgcg ctcgtgcagg gtcttcagcg
     4621 cgcggaagac gaccgccgtc ggcaggctct cgtagtcttc cgcttcgagc tgcgggagga
     4681 tcgcgccgcg cagctccgcg tcgtgaacga gcagctccag cagcctctgc tcggcgagcg
     4741 tcgggtggcc ggctgaggcc cgcgcgacct tctgcttcac gtccgaaggg gccggggcgc
     4801 tggcgcccgc cgcgctcacg gccttccaca actcctgccg cagagacttc tcctcgacgc
     4861 gcagcgcgcc catcgccatg tcgaaatact cccgcttctg gatgcggttc ctgacggcgc
     4921 gcaggtaagg cagcacctct tccacggcct ctgccttgtg cgccgggacg cgcaggtcgc
     4981 gcccttcgag cgcctggtcg atgacgaagt tgatgtaagg aaacgcctcg ccgcgccggc
     5041 ggttgtactc ttcggcgccg tgcgcgcgca ggaactcgtc cgggtccgcg ccctcgggca
     5101 gcaccagcac tttgatctcg aagtcttcgg ccagcagcgt ctcgatggcg cgcttcgcgg
     5161 ccttcacgcc cgccttgtcg ccgtcatagt taaccaccac tttgcgcgca aagcgaccca
     5221 gcaggcgcac ctgctccggc gtcagggcgg tgcccagact cgccaccacg ttccgcacgc
     5281 cgaactggta gggcacgatc agatcgagat agccctcgac gaggatgacg aactttttct
     5341 tccgtatctc ttcgcgcgac acgttcagcc cgaacaggtg acgccccttc gtgtaggccg
     5401 ccgtctccgg cgagttcaga tacttcggct cgtcgcccgc gcggatggcc cgcgcgccga
     5461 acgccacggg ccttccttgc gcgtccatca cggggaagac gacgcgcccg cggaagcggt
     5521 cgtaaaagcc gcccgcgtcc ttcttcacga cgaggccgct ccgctcgatc tgcgcgacgc
     5581 tcgcgccctt gctcttaaga tagttgccga gcgcgtccca actgtccggc gcgtaaccca
     5641 tgcggaagac tttctgcgtc tcctccgaga tgccgcgccc ctcgacgtac tcgcgcgccg
     5701 cctgcgcctc ctgcccttcg gccaactgcc gctcccagaa ctccagcgcc cacccgttga
     5761 gctggacgac ctcgtccgcc tccttgcgcc gcgactcgta ccccttgtcg tccaccatca
     5821 cgggcagcgg cacgcccgtc ttctccgcca cgatgcgcac cgcctcgggg aacgacacgc
     5881 gctccaactc catcacgaag ttgaagacgc tccccccctt cgcgcagccg aagcagtaga
     5941 agatgtcctt cgacgggctg accgagaatg acggcgactt ctcctgatgg aacgggcagc
     6001 acgccatcca gttcgcgccc ttctttttca acgtcacgta atccgagatc acgcgcacga
     6061 tgtcggcctg ccgccggagg tcgtcgatga acacttgcgg gaatcgcatc tctcttcgac
     6121 ttgggcttat tcagtcgggg cgctgaattc ggcgacgact tcaatcgggc cgacgaggtt
     6181 gcggttgaac tcgggcaact cttcggcggg aacccagtac tcctgatgga ccgagccgcc
     6241 gaccgtgtgg acatcatagc gctcaagaaa cgcgcggcgc acgcggaagc gtgtgacgaa
     6301 gccgcgcccg cccgcgttgc gtctcgcgtt ccagtcgcgg gcgatctgcg tggcgtactc
     6361 ttcgttgagc acggggtaga agatgggctg ctccggcagg cgcggcggga actcgcgcca
     6421 atcgctcgcg cgaatcagct ccaactcctt cggcccgacg gggcggaaga gcatgacggt
     6481 ctcgtcgcct ctcatcactg cctgctcttc gtccgtcatc actgcttcag ctcgaccacg
     6541 gtcgcgcccg cgccgccctg attgtcgggg gcggcggcga agcgcgcgac gtgcgggtgc
     6601 gtgcggagga gttcggcgac ggcgcggcgg agcgcgcccg tgccgtggcc ggggatgatg
     6661 cggacgtgcg cgaggccgtg aaggtatgcg tcgtccaaaa acttgtcggt ctcgtccgcc
     6721 gcctcgtccg tggtcttgcc gatgaggttg agttcggcgt cgggcgcctc gcgcgcctgt
     6781 ttgctgaggt gcacttcggt gccgcgcttc tgcatgtcgc gcaggcgcgc ggcgagcgag
     6841 tcgtctttct gctcgggcgc gggcgcgacg agttcgaggt tcctgacctt ctcgcggaag
     6901 cgcaggtgct tgacgcgcac ttcggcctcg ctcccgctga cgcgttcgac gacgccctcg
     6961 gagccgagcg tgcgcagacg cacgcggtcg ccgacgacga tctcacgctg cggcacggcg
     7021 agagcgtcgg acgaagggac ggcgtcgcct tcatcggcgc gcgcgccggg gcggcggacg
     7081 cggacgccgg agccggtctc gcgtgtctgt ggcgaggcgg tcgcggcgcg cgcctgcgtg
     7141 cgctgggcct gcgcctcgcg gcgcagctcg gcggcgcggc gctcggcctc gcgctcgacg
     7201 cggacgcgct cggcgcggtc ttcgatcttc gagtacagct cgcgcgcgcg cagctcgaag
     7261 tcggcgatgg ccctttccag ctcgcgcgcg aaagacgctt gccgctcgcg ctccctgcgc
     7321 tcggcgtcgc ggtcgagcgc ggcgtacttg tctgcgacgg cgcggcgctc ctcttcgagc
     7381 gcgcggcgca atccttcggc ctcttcggac tcctgcttga tgcggcggag gtattcggcg
     7441 gcggcgagcg acttctcgtc cacgaactcg cgcgcggcgc cgatgacgcg tgcggggatg
     7501 ccgaagcgtt gcgcgatgtc gagtccggcg gacgagccgg cgaggccgac gagcaggcgg
     7561 taggtcggct gcaaagtctt ctcgtcgaac tcgacgctcg cgttctggac gccctcctcg
     7621 ttggccgcgt acatcttcag cccgctgtag tgcgtcgtcg cgacgacgtg cgcgccgcac
     7681 gcgcggcgga agtgatcgac taccgccacg ccgagcgccg agccttcctc ggggtctgtg
     7741 ccggtgccga cttcgtcgag caggacgagg gcgggccgct cgcacagctc gatcatgcgg
     7801 cggatgttgg cgacgtgcga ggtgaaggtc gagaggttcg cggcgagcga ctggcggtcg
     7861 ccgatgtcgg cgagcaccga ggcgtagagc ggcacgcgcg ccgcccgcgc cgcgacgtgc
     7921 aggcccgaga gcgccatcaa cgaagagagg cccgccgtct tgagcaccac cgtcttgccg
     7981 cccgcgttgg cgcccgagat gaccatcacg ggccgcgccg cgtcgaggct cagcgagacg
     8041 ggcaccacct cgcgcccctg cgctctgagg ttctcttcga gcagggggtg tcttgcttct
     8101 tcgagttcga gcgtctcgtc gtcgctgatc gcaggctccg tgcagcggag gtggttcgag
     8161 agcgtcgcct tcgcgccgac gaagtcgagt tcggcgacgg cgcgcgccgc ctcttcgatg
     8221 gagccgatct cggcgcgcag ctcttcggtg agcgtgaaga ggatgcgcgt gatctcgcgc
     8281 tcctcggtct cgcgcaaggc ttgcagctcg ttgttggact cgatggtttc gagcggctcg
     8341 acgaagaccg tggcgcccga agaggagaag ccgtgcgcga cgccgtggac gcgcccgcgg
     8401 tggtcgctgc ggacggggat cacgaagcgg tcgttgcgca ccgtcacgag ctgatcctga
     8461 atggcctcct ccgag
//
LOCUS       DBNGOH010000460         4683 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_252771, whole genome shotgun sequence.
ACCESSION   DBNGOH010000460 DBNGOH010000000
VERSION     DBNGOH010000460.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4683)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4683)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4683
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_252771"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1889
                     /locus_tag="AC159P_09085"
     CDS             <1..1889
                     /locus_tag="AC159P_09085"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR3695646.1"
                     /translation="AAGEGYSGGAGDVSSAPSEPLGDEDSKTLDAPPAEPPGDAGSGG
                     INPQFQPTIIDGPERNGGTGDEPARAQDPAADEAAAPVVNTGPQSGTLDEPHESHVTT
                     APEPAAPADSPAPYDSADIVKPLPIEMTIDRIAPSFDDAGAERRVTSDEAEARRHTGD
                     QSRPIGGTAGASGGRRKGAAQLEPGTVLYARYEIVRKIGGGGMGAVYFAKDRNLGDAP
                     RAVKEMVQSNLDEGQHEKAINDFKRESMLLASLEHPSIPTIYDYFFDEGSTRFYLVMK
                     YISGGDFLARLRNAPGGRIDELTVADWGMQVADVLDYLHNRRPPIIYRDLKPANLMID
                     GNTGRVMLIDFGIARWVAPQEKGVTAVGTMGYAPPELFSGKVEPRSDVYSLGATMFHL
                     LTGADPQDNPLLIFDFNKFPRPRQINPGLSNEMERILMRAVEYTPETRFRSAAELRDT
                     LAHHTEKLRAGAVTYGAVQSANDPNAVKTVGVEMVYCGFCGGRIAAEDVFCAHCGARQ
                     PISAASVRAARATAKLVVAETTALDATFQLQKDSILVGRTDPQSNIFPDVDLSKFDPE
                     TKISRRHARIWRQGETFLVEDLGSVNGTVINDSVKLGPRQPRTLEHGDRLRLGETTLH
                     FLVG"
     gene            1982..2503
                     /locus_tag="AC159P_09090"
     CDS             1982..2503
                     /locus_tag="AC159P_09090"
                     /inference="COORDINATES: protein motif:HMM:NF018028.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HIR3695647.1"
                     /translation="MRIEQPAAPRAAARRTPARVERRAPDANFERLRAPFSLRCGAVL
                     IDYIVLVSIIALATMLERVSGDGRKGGSTLLFASYAVAAATAFLNLVVLPAWTGRSLG
                     KWVTALRIERRDGVPLSIGRALLRHLLGYPLTLLTLGAGFLLAAFSAEGRALHDLLAG
                     TVVVRSRGGNAER"
     gene            2591..4261
                     /locus_tag="AC159P_09095"
     CDS             2591..4261
                     /locus_tag="AC159P_09095"
                     /inference="COORDINATES: protein motif:HMM:NF024821.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3695648.1"
                     /translation="MTKHCPSCGAEIQQGARFCRRCGAPVRNVAGGDGDDDVSPQAAT
                     VPLRDEGRTTDGLAADDPRRASADTTRVNRDELERLLQTQQSQRTLDAAGTAGAAPPE
                     PQAPTDTRAGDAPNTGELPLPGGASEDLLKTRASEDLLKTRASFAFQDDDAEDLTVIA
                     PPRTSRPYDEDDGARRVEPSPPHTGPPPPTGGAVAHPTAPPPRPPRSKWPYVIAACVV
                     LLFMAAGVALVAVMYLRRQAPTAVVPQAPTPPPDVAQLFEEKLAQAESSMAAGQFDAA
                     VAALREANQLDPANWKAHRRLGELLWESGQRREAIEEYRAAATNNPNDFTLWRALASA
                     EAAEGLQQEAAESYKRLVDLVGEAAADPNDLLAYADALRLAGRAEEARGVYQKLVSVA
                     ATDVAAAARLRLAELAQPTPSPTPGQQGNTNTRPDATPTPANSQATPTPPPAATPTPP
                     PPTPAPTRPAELSPADSYQRGVQLWSSNRGEALRLFRAAAGAGNPDAHFYLGLGYVEG
                     RDLGSLKRAEIGAALQHFQNAQRGQHAARARQYALQLEREFDRIRNQQ"
     gene            4417..>4683
                     /locus_tag="AC159P_09100"
     CDS             4417..>4683
                     /locus_tag="AC159P_09100"
                     /inference="COORDINATES: protein motif:HMM:NF028007.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HIR3695649.1"
                     /translation="MPELIVNYPDRASDVLPLGRLRITIGRSARNDVCVPDPFASRVH
                     AEVRREGDLYYLQDLGSANGTYYNGSRLDQPVMLTHGGRIQIGET"
BASE COUNT          763 a         1805 c         1502 g          613 t
ORIGIN      
        1 ccgccgccgg cgaaggttac tccggcggcg cgggggacgt gtcctccgcg ccctccgagc
       61 cgctcggcga cgaagactcg aaaaccctcg acgcgccgcc cgcggagcct ccgggcgacg
      121 ccggcagcgg cggcatcaac ccgcagttcc agccgaccat catcgacggc cccgagcgaa
      181 acgggggcac cggcgacgaa cccgcgcgcg cacaagaccc cgccgccgac gaagccgccg
      241 cgccggtcgt caacaccggc ccgcagtccg gcacgctcga cgagccgcac gagtcgcacg
      301 tcaccaccgc gcccgaaccc gcggcgcccg ccgattctcc ggccccctac gactccgcgg
      361 acatcgtcaa gccgctgccg atcgagatga cgatcgaccg catcgcgccg tcgttcgacg
      421 acgcgggggc cgagcgccgc gtgacttcgg acgaggcgga ggcgcggcgc cacacgggcg
      481 accagtcgcg gcccatcggc ggcacggcgg gcgcgagcgg cgggcggcgc aagggtgccg
      541 cgcagctcga acccggcacc gtgctctacg cacgctacga gatcgtccgc aagatcggcg
      601 gcggcggcat gggcgccgtc tacttcgcca aagaccgcaa cctgggcgac gcgccgcgcg
      661 ccgtcaagga gatggtgcaa tcgaacctcg acgaggggca gcacgagaag gcgatcaacg
      721 acttcaagcg cgagtcgatg ctcctcgcct cgctcgaaca cccctcgatc ccgaccatct
      781 acgactactt cttcgacgaa ggctcgacgc gcttctacct cgtcatgaag tacatctcgg
      841 gcggcgactt cctcgcgcgc ctccgcaacg cgcccggcgg tcgcatcgac gagctgaccg
      901 tggccgactg gggcatgcag gtcgcggacg tgctcgacta cctgcacaac cggcggcccc
      961 ccatcatcta ccgcgacctc aagcccgcga acctgatgat cgacggcaac acgggccgcg
     1021 tcatgctcat cgacttcggc atcgcgcgct gggtcgcgcc gcaggagaag ggcgtcaccg
     1081 cggtcggcac gatgggctac gccccgcccg agctgttcag cggcaaggtc gagccgcgct
     1141 cggacgtgta ctctctgggt gcgacgatgt tccacctcct gacgggcgcc gacccgcagg
     1201 acaacccgct gctcatcttc gacttcaaca agttcccgcg cccgcgccag atcaatccgg
     1261 gcctctcgaa cgagatggag cgcatcctga tgcgcgccgt cgagtacacg ccggagacgc
     1321 gcttccgctc cgccgccgag ctgcgcgaca cgctcgcgca ccacacggag aagctgcgcg
     1381 cgggcgccgt cacctacggc gcggtgcagt cggcgaacga cccgaacgcg gtgaagaccg
     1441 tgggggtcga gatggtctac tgcggcttct gcggcggcag gatcgcggcg gaggacgtgt
     1501 tctgcgcgca ctgcggcgcg cgccagccca tctcggcggc gagcgtgcgc gcggcgcgcg
     1561 ccaccgccaa gctcgtcgtc gccgaaacca ccgcgctcga cgccacgttt cagttgcaga
     1621 aggacagcat cctcgtcggg cgcaccgacc cgcagtcgaa catcttcccc gacgtggatc
     1681 tgtcgaagtt cgaccccgag acgaaaatct cgcgccgcca cgcgcgcatc tggcggcagg
     1741 gcgagacctt cctcgtcgaa gacctcggct cggtcaacgg cacggtcatc aacgactcgg
     1801 tcaagctcgg cccccggcag ccgcgcacgc tcgaacacgg cgaccgcctc cgcctcggcg
     1861 agacgacgct gcacttcctc gtcggatgaa atcggaagtc ggaagtcgga atataagagc
     1921 cgttgctgcc gttccattcc gacttccgac ttctgacttc cgactccttg attccatgtc
     1981 tgtgcgaata gaacaacccg ccgcccctcg tgccgccgcc cgccggacgc ccgcccgcgt
     2041 cgagcggcgc gcgccggatg cgaacttcga acgcttgcgc gcgcccttct cgctgcggtg
     2101 cggcgcggtg ctgatcgact acatcgtgct cgtctcgatc atcgcgctgg cgacgatgct
     2161 ggagcgcgtc tcgggcgacg ggcgcaaggg cggctcgacg ctgctcttcg cttcttacgc
     2221 ggtggcggcg gcgacggcgt tcctcaacct cgtcgtgctg cccgcgtgga cgggccgctc
     2281 gctcggcaag tgggtgacgg cgttgcgcat cgagcggcgc gacggcgtgc ctctgagcat
     2341 cgggcgcgcg ctgctgcgcc acctcctcgg ctacccgctg acgctcttga cgctcggcgc
     2401 cggcttcctc ctcgccgcct tcagcgccga agggcgtgcc ctccacgacc tgctggccgg
     2461 caccgtcgtc gtcaggagcc gcgggggaaa tgcggaacga tgaatgatga atgatgaatg
     2521 aaaggcagct tgaacgataa agttcggcgt gcttcccatt catcatccat cattccgcat
     2581 tcatcatttt atgaccaagc actgtccaag ctgcggcgcg gagatacaac agggcgcgcg
     2641 gttctgccgg cgttgcgggg cgccggtgcg taacgtcgcg ggcggcgacg gcgacgacga
     2701 cgtgtcgccg caggcggcga ccgtcccgct cagggacgag gggcgcacga ccgacggcct
     2761 cgcggcggac gacccgcgcc gcgcctcagc cgacacgacc cgcgtcaacc gcgacgaact
     2821 cgaacgcctc cttcagacgc agcagtcgca gcgcacgctc gacgccgcag gcaccgcggg
     2881 cgccgccccg cccgaacctc aggcgccgac cgacacgcgc gcgggcgacg cgccgaacac
     2941 gggcgagctg ccgctgcccg gcggtgcgtc cgaagacctc ctgaagacgc gcgcgtccga
     3001 agacctcttg aaaacgcgcg cgtccttcgc cttccaggac gacgacgcgg aagacctgac
     3061 cgtcatcgcc ccgccgcgca cctcgcgccc ctacgacgaa gacgacggcg cgcgccgcgt
     3121 cgagccgtcg cctccgcaca caggcccgcc gcctccgacc ggcggggcgg tcgcgcaccc
     3181 gacggcgccg ccgccgcgcc cgccccggag caagtggccc tacgtcatcg ccgcctgcgt
     3241 cgtcctgctg ttcatggcgg cgggcgtcgc actcgtcgcc gtcatgtacc tgcggcggca
     3301 ggcgccgacg gcggtcgtcc cgcaggcgcc cacgccgccg cccgacgtcg cgcagctctt
     3361 cgaggagaaa ctcgcgcagg ccgaatcctc gatggccgcc gggcagttcg acgcggcggt
     3421 ggccgcgctg cgcgaggcga accagctcga ccccgccaac tggaaggcgc accgccgcct
     3481 cggcgagctg ctgtgggaga gcggccagcg gcgcgaggcc atcgaagagt accgcgcggc
     3541 ggcgaccaac aatccgaacg acttcacgct ctggcgcgcg ctcgcctccg ccgaggccgc
     3601 cgaagggctg caacaggaag ccgccgagag ctacaagcgt ctggtcgatc tcgtcggcga
     3661 ggcggcggca gacccgaacg acctgctcgc ctacgccgac gcgctgcgcc tcgcgggccg
     3721 cgccgaagag gcgcgcggcg tctaccagaa actcgtctcc gtcgccgcga ccgacgtggc
     3781 cgccgccgcg cgactgcgcc tcgccgaact cgcgcagccg acgcccagcc ccacgcccgg
     3841 acagcaaggc aacaccaaca cgcgccccga cgcgaccccg acccccgcga attcgcaagc
     3901 gacgccgacg ccgccgcccg cagcgacgcc cacgccgcca ccgccgacgc ccgcgcccac
     3961 gcgccccgcg gagctttcgc ccgccgactc ctaccagcgc ggcgtccagc tctggtcttc
     4021 gaaccgcggc gaggcgctcc gcctcttccg cgcggcggcg ggcgccggca acccggacgc
     4081 gcacttctac ctcggcctcg gctacgtcga aggccgcgac ctcggctcgc tcaagcgcgc
     4141 cgagatcggc gccgcgctcc agcacttcca gaacgcacag cgcggccagc acgccgcccg
     4201 cgcgcgccag tacgcgctgc aactcgagag ggagttcgac cgaatcagaa accaacaata
     4261 aaggactgag gactgaggac tgagtaacag ccgtgaactg tgaaccgtga accgtgatga
     4321 gtccggccag cgtgcctctc gtcattcgtc atcggtcaca cgctatttgc ttttactcag
     4381 tcctcagtcc tcagtcccca gtccttcgaa tcgaccatgc cggaactcat cgttaactac
     4441 cccgaccgcg cttcggacgt gctgccgctc gggcgcctgc gcatcaccat cgggcgctcg
     4501 gcgcgcaacg acgtgtgcgt ccccgacccc ttcgcctcgc gcgtccacgc cgaggtcagg
     4561 cgcgagggcg acctttacta cctgcaggac ctcggctcgg ccaacggcac ctactacaac
     4621 ggctcgcgcc tcgaccagcc ggtcatgctc acgcacggcg ggcgcatcca gatcggcgag
     4681 acc
//
LOCUS       DBNGOH010000461         3014 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_619394, whole genome shotgun sequence.
ACCESSION   DBNGOH010000461 DBNGOH010000000
VERSION     DBNGOH010000461.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3014)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3014)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3014
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_619394"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2425)
                     /locus_tag="AC159P_09105"
     CDS             complement(<1..2425)
                     /locus_tag="AC159P_09105"
                     /inference="COORDINATES: protein motif:HMM:NF051698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR3695650.1"
                     /translation="MKRQTPVKGRAAAALLWALALTLFAGPGTFAQTGTSSIRGTVTD
                     QQGGVIAGANVTLTSAEKNFTRTQVTTEDGGYLFNTVPPGAYRVVVEAAGFKRSVAEV
                     TAQVDTPLDLDMQMEPGSITESVTVTAADTAPLNTTDATIGNAFESRRIQDLPLNARN
                     VVGLLSLQPGVTRGGDVNGGRRDQANITLDGVDVNEQQRGVDAVTDQAFASVLRVTPD
                     SVQEFRVITTNANAEQGRSSGAQVSLVTKSGGNDYHGVLYHYHRNTVTTANDFFNNAA
                     GNFGPNDPQVLSGAARVGDPRVPRPQLLRNIFGGALGGPIKKDRAFFFFTYEGFREAT
                     STSVVRLVPLPQSLGQGLVRYATSSSTAGSPCPIQSEPNRRCISLTPAEINAAYLAAN
                     GVSPGVSTAALNLLRDAAQRYPANDFTTGDQINTGGFRFNARTPTELDTYIGRLDFNL
                     TDRQSLFVRGNYQDDLVGQAPQFPDTPAPNIWYHPKGIAVGHSWTASNNFVNRATYGL
                     TRLAFTQGGDSTDPSLSFRFIFTPLLYSRALTRITPVHNFVDDISWIRGNHNLQFGGN
                     VRFIKNTRNSFANSFDSLTTNPSGYNASGAVLTSAGADAGGPAIFSSVATTSVGPLRN
                     ALAAVIGRFSQYSANFLYDADGGILPSGQSSDRTFATQEYETYFQDSWRWKPNVTLTY
                     GVRWSTSTPVYETNGFQVVPTVSLGDYFERRVASADRGVPFNELITLDRGGKFYGKPG
                     FYEQDWNNFAPSIAFAYSPDFGDNFLGRLLGREGKSVIRGGFRMTYDRIGSQLAVSFD
                     LNNTLGFASA"
BASE COUNT          471 a          906 c         1120 g          517 t
ORIGIN      
        1 ccgccgaggc gaacccgagc gtgttgttga ggtcgaagga gacggcgagc tggctcccga
       61 tccggtcata ggtcatgcgg aagccgccgc ggatgacgct cttgccctcg cgcccgagca
      121 ggcgcccgag gaagttgtcg ccgaagtcgg gcgagtaggc gaaggcgatg gacggcgcga
      181 agttgttcca gtcctgctcg tagaagccgg gcttgccgta aaacttgccg ccccggtcga
      241 gcgtgatcag ctcgttgaac gggacgcccc ggtcggcgct cgcgacgcgc cgctcgaagt
      301 agtcgcccag cgagacggtc ggcacgacct ggaacccgtt cgtctcgtag acgggcgtcg
      361 aggttgacca cctcacgccg taggtcagtg tcacgttggg tttccagcgc caactgtcct
      421 ggaagtaggt ttcatactcc tgtgtggcga aggtgcggtc ggaggactgg ccggaaggca
      481 gaatcccgcc gtcggcgtcg tagaggaagt tcgcgctgta ctgcgagaag cgcccgatga
      541 cggcggccag cgcgttgcgc agagggccca cggaggtggt ggccacgctc gaaaagatcg
      601 cggggccgcc ggcgtccgcg ccggcggaag tcagcaccgc gccggaggcg ttgtagcccg
      661 acgggttggt cgttagcgag tcgaaggagt tggcgaacga gttgcgcgtg ttcttgatga
      721 aacgcacgtt gccgccgaac tgcaagttgt ggttgccgcg aatccacgag atgtcgtcca
      781 cgaagttgtg caccggcgtg atgcgcgtga gcgcgcgcga gtagaggagg ggggtgaaga
      841 tgaagcggaa actgagcgag ggatcggtcg agtcgccgcc ctgcgtgaag gccagccgcg
      901 tgaggccgta ggtggcgcgg ttgacgaagt tgttcgaggc cgtccacgag tggccgaccg
      961 cgatgccttt cgggtgatac cagatgttgg gcgcgggggt gtcggggaac tggggcgcct
     1021 gtcccaccag atcgtcctgg tagttgcccc gcacgaagag cgactggcgg tcggtcaggt
     1081 tgaagtcgag gcgtccgatg taggtgtcga gttcggtcgg cgtccgcgcg ttgaagcgga
     1141 agccgccggt gttgatctgg tcgcccgtcg tgaagtcgtt ggccggatac ctctgcgcgg
     1201 cgtcgcgcag gaggttgagg gccgccgtgc tgacgccggg gctgacgccg ttcgccgcca
     1261 ggtaggcggc gttgatctcg gcgggcgtca gcgagatgca gcgccggttc ggctcggact
     1321 ggatcgggca cgggctgccg gcggtggagc tgctggtggc gtagcgcacc agcccctggc
     1381 cgagcgactg gggcaggggc accagccgca ccacgctcgt gctggtcgcc tcgcgaaagc
     1441 cctcgtaggt gaagaagaag aatgcccggt ccttcttgat ggggccgcca agcgccccgc
     1501 cgaagatgtt gcgcaaaagc tgcgggcgcg gcacgcgcgg gtcgccgacg cgtgccgcgc
     1561 cggagagcac ctgcgggtcg ttcgggccga agttgcccgc cgcgttgttg aagaagtcgt
     1621 tggcggtcgt caccgtgttg cggtggtagt ggtagaggac gccgtgataa tcgttgcccc
     1681 ccgacttggt gacgagcgag acctgcgcgc cggaggagcg cccctgctcg gcgttggcgt
     1741 tggtcgtgat gacgcggaac tcctgcaccg agtcgggcgt gacgcgcagc acgctggcga
     1801 aggcctgatc cgtgacggcg tcaacgccgc gctgctgctc gttcacgtcc acgccgtcga
     1861 gcgtgatgtt ggcctgatcc cggcgtccgc cgttgacatc gccgccgcga gtcacgccgg
     1921 gctggagcga taacaggccg acgacgttgc gggcgttgag cggcagatcc tgaatgcgcc
     1981 ggctctcgaa ggcgttgccg atagtcgcgt ccgtggtgtt cagcggcgcc gtgtccgcag
     2041 cggtcacggt gacggactcg gtaatactgc ccggctccat ctgcatgtcg aggtcgagcg
     2101 gggtgtcaac ctgggcggtc acttcggcca ccgatctttt gaaccccgcg gcctcgacca
     2161 cgacgcggta agcgccgggc ggcaccgtgt tgaagaggta gccgccgtct tcggtagtga
     2221 cctgcgtccg ggtgaaattc ttctccgcgc tggtcagcgt cacgttggct cctgcgatga
     2281 cgccgccctg ctgatccgtg accgtccctc gaatcgagga cgtgccggtc tgcgcgaacg
     2341 tgccggggcc ggcgaacagg gtgagggcga gcgcccacag caaagccgcc gcggcgcgtc
     2401 ccttaacggg ggtctgtctt ttcattgtcc ctcctcagta aaagtgtcgg cgcgcccgca
     2461 gcagaccgcc ggcggtctgc tgcgggacgc ggagaacgga aagtgggctt ggaacgtttg
     2521 acggactccg ggcggcgtcc gggaagttcg gcgacgccgc cggtgcttta acgcttgcgt
     2581 gctcttcgtc ccgcgcgccg cgcgcgacga agatcgagga tttataaaga cagggcggcg
     2641 ctacggatcg agccgccgcg aacgtatgaa gaaataccgg cgtccgggac gcccggcgag
     2701 acagtcccgc cgttgacact tattaaggac gcggcgggag ttgattcact cgcgtggccg
     2761 accggcccgc gcccgccccc cggcgggcga caggtctcgg gcggtcactc gctggtgctt
     2821 cacgccgcga cttccatgga tgggcgcggg cattatatcc aaaaatcggt acccggtaca
     2881 attttttaga cttcccccgg agcagggcgc aaggggggct ggcgggcggc aaagacgagg
     2941 gcgcccgcgt cgccggccct gcgtgagggg ggccgggggc gcgggcgcct gtccgtctcg
     3001 tatccttatt aaat
//
LOCUS       DBNGOH010000462         3569 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2269305, whole genome shotgun sequence.
ACCESSION   DBNGOH010000462 DBNGOH010000000
VERSION     DBNGOH010000462.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3569)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3569)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3569
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2269305"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1763)
                     /locus_tag="AC159P_09110"
     CDS             complement(<1..1763)
                     /locus_tag="AC159P_09110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolB family protein"
                     /protein_id="HIR3695651.1"
                     /translation="MSPRTAGTPARRPVARPRQRHLGSRTHTLSTRLASAAALLLLAA
                     FAALAQTTYRKPPQAVLDVLNAPVPPAASISPARDYMLLLTGVRYPPISELAQPMLRL
                     AGLRINPNISGPHRAPTFVAVSVKKISDGTETKVALPAEAQAGVPAWSADGRHFAFTN
                     TTPAGLELWVGETATAKVRKIKNVAVNAVYGEPLQWMPDGKTLLVQLVPAKRAAAPVA
                     PGVPVGPNEQESTGRATGAATFQDLLKNPHDADLFDYYATAQLALVDATGGKVTPLGS
                     PSVYEEAAPAPDGRHLLVTRVHRPYSYLHPAFAFPREIEVWDTAGKSVHKVASVPLAD
                     NVPLQGVRTGPRAVQWRPTAPATLLWAEALDGGDPRKKVTHRDRLMTLSAPFKSPATE
                     LFKVEHRYSGAQWGERGGLLLVSDYDRDKRWTRTQAIDADSPAAAPRTIFSRSINDQY
                     GNPGTPVTRLLPTGHRAVLQHGDAIYLAGAGATPEGDRPFLDRFSLATLKGERLFRSD
                     PDAYETFAALLADDASRIVTRRERPISPANYYVRTLGGQGGDAAALRPLTDVKDATPQ
                     LQGIKKQLIKYKRADGVDLSFT"
     gene            1953..2873
                     /locus_tag="AC159P_09115"
     CDS             1953..2873
                     /locus_tag="AC159P_09115"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006677744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HIR3695652.1"
                     /translation="MNVRRVVCESIVWLALVLSLGGAPHFPASAQRDRRAAAQPFRVM
                     TYNIRYDDPRAGQLAWANRREMVASMIRFHQADIVGLQEALANQLADLERMLPGFVAF
                     GAGRDDGGRKGEFSAILYNKARFRFLSGKTFWLSETPAVAGSRGWDAAYPRVVTWAKF
                     EERATGKTFFHFNTHFDHRGERARVESARLLLRSVWRIAGRSAPVIVTGDFNFNEAAE
                     GYRILTGKADAQSPGARVLHDARYLSLHGHHGPTATFNDFKALVPDSKIDHVFAGKGV
                     KVRQHGVLSDIWDGRFPSDHFPVLAELVLD"
     gene            3340..>3569
                     /locus_tag="AC159P_09120"
     CDS             3340..>3569
                     /locus_tag="AC159P_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HIR3695653.1"
                     /translation="MAFKSLFSLFSSDLAIDLGTANTLVYAKGRGIVVSEPSIVAINK
                     VTNLPEAVGKEAKEMLGRTPGNIVAIRPMKDGV"
BASE COUNT          538 a         1142 c         1276 g          613 t
ORIGIN      
        1 gtgaacgaga ggtcaacgcc gtcggcgcgc ttgtatttga tgagctgctt tttgatgccc
       61 tgcagctggg gcgtcgcgtc cttcacgtcg gtgagcgggc ggagcgccgc cgcgtcgccg
      121 ccctgcccgc cgagcgtgcg gacgtagtag ttcgcggggc tgatggggcg ctcgcggcgc
      181 gtgacgatgc ggctcgcgtc gtccgccagc agcgcggcga acgtctcgta ggcgtccggg
      241 tcgctgcgga agaggcgctc gcccttgagc gtggcgagac tgaagcggtc gaggaacggg
      301 cggtcgcctt cgggagtggc gcccgcgccg gcgaggtaga tcgcgtcgcc gtgctggagc
      361 acggcgcggt ggcctgtcgg caggaggcgc gtgacgggcg tgccggggtt gccgtactgg
      421 tcgttgatgc tgcggctgaa gatcgtgcgg ggcgcggcgg cggggctgtc ggcgtcgatg
      481 gcctgcgtgc gcgtccagcg cttgtcgcgg tcgtagtcgg agacgaggag gaggccgccc
      541 cgctcgcccc actgcgcgcc ggagtagcgg tgctcgacct tgaaaagctc cgtggccgga
      601 ctcttgaagg gcgcgctcaa ggtcatcaga cggtcgcggt gggtgacctt cttgcgcggg
      661 tcgccgccgt cgagcgcctc ggcccagaga agcgtggcgg gcgcggtcgg tctccactgg
      721 actgcgcgcg ggccggtgcg cacgccctga agcgggacgt tgtccgcgag cggcacgctc
      781 gccaccttgt gcacggactt gcccgcggtg tcccagacct cgatctcccg cgggaaggcg
      841 aaggccgggt gcaggtacga gtaggggcgg tggacgcgcg tgaccagaag gtgcctgccg
      901 tcgggcgcgg gcgcggcctc ttcgtagacg gaaggactgc cgagcggcgt caccttgccg
      961 cccgtcgcgt caaccaaggc gagctgcgcc gtcgcgtagt agtcgaagag gtctgcgtcg
     1021 tgcgggttct tcaggaggtc ttggaaggtt gcggcgccgg tcgcgcggcc cgtgctctcc
     1081 tgctcgttcg gccccacggg gacgcccggc gcgacgggcg ccgccgcgcg cttcgcgggg
     1141 acgagctgca cgagcagcgt cttgccgtcg ggcatccact gcaacggctc gccgtagacg
     1201 gcgttgacgg cgacgttctt tatcttccgc accttcgccg tcgccgtctc gccgacccac
     1261 agctcaaggc cggcgggcgt cgtgttggtg aaggcgaagt gtctgccgtc ggcgctccac
     1321 gcggggacgc ccgcttgcgc ctcggcgggg agggcgacct tcgtctcggt gccgtcggag
     1381 attttcttga ccgagacggc gacgaaggtg ggcgcgcggt gcggccccga gatgttcggg
     1441 ttgatgcgca ggcccgcgag ccggagcatc ggctgcgcca gctccgagat gggcgggtaa
     1501 cgcacgcccg tcagcaggag catgtagtcg cgcgccgggc tgatggaagc ggcgggcggc
     1561 accggcgcgt tcagcacgtc gagcacggcc tgcggcggct tcctgtaggt cgtctgcgcg
     1621 agggccgcga aggccgcgag cagcaggagc gcggcggcgg acgcgagtcg cgtcgaaagg
     1681 gtgtgcgtgc gggagccgag gtggcgttgg cgcgggcgtg cgacggggcg gcgcgcgggc
     1741 gtgcctgcgg ttcgagggga catctcctgt ttcctccggg ttggcgtggt cggttatgtt
     1801 tgcgggcgtg cgtctcgaac gcggatttta actcgacagc gcccgattat gaaaatccgc
     1861 cggaggtgct gtcgagctgt gctaacctga ggcgttccga actggattaa cgccgtcccg
     1921 agcgcctgct ccggctgtcc gtggggaaag tcatgaatgt cagaagggtg gtctgcgagt
     1981 cgatagtgtg gctggcgctg gtgctgtcgc tgggcggcgc tcctcacttc ccggcgtccg
     2041 cgcaaagaga caggcgcgcc gccgcacagc ccttcagggt catgacctac aacatccggt
     2101 acgacgaccc gcgcgcgggc cagctcgcgt gggcgaaccg ccgggagatg gtcgcgagca
     2161 tgatccgttt ccatcaagcg gacatcgtcg gtttacaaga agccctcgcc aaccagctcg
     2221 ccgacctcga acggatgctc cccggcttcg tcgccttcgg ggccggtcgt gacgacggcg
     2281 ggcgcaaagg tgagttttcg gcgatcctct acaacaaagc gcgtttcaga ttcctttccg
     2341 gcaagacctt ctggttgtcc gaaacgcccg ccgtcgccgg gagccgcggc tgggacgcgg
     2401 cctacccgcg cgtcgtcacg tgggcgaagt tcgaggagcg ggccaccggc aagaccttct
     2461 ttcatttcaa cacgcacttc gaccacaggg gcgagcgcgc ccgcgtcgag agcgcgcggc
     2521 tgctgctgcg aagcgtctgg aggatcgcgg gacgttccgc cccggtcatc gtgacgggcg
     2581 atttcaactt caacgaggcg gcggagggct accgcatcct gacgggcaag gcggacgctc
     2641 agagtccggg ggcgcgcgtg ctgcacgacg cacgctacct ttcgctccac gggcaccacg
     2701 gcccgacggc caccttcaac gacttcaagg cgctcgtgcc cgactcgaaa atcgatcacg
     2761 tcttcgccgg caagggcgtc aaggtgcggc agcacggcgt gctgtccgac atctgggacg
     2821 ggcgcttccc gtcggatcat tttcccgtcc tcgccgagct ggtgctggac taagcctctg
     2881 ccggtctccg aagacgggcg cgggccgcct gtcctctctc gctcccttat ccatcagttg
     2941 aaacagcccg caaacgcttc tcggggaagg gggatgcaaa aaacgtacac gggcggataa
     3001 aaagttgtag acatttcccg cgccatgctg taaagcaata tcgagttgtc cagagttgca
     3061 ataccccccc gcccggcaat gtcataagcc ccgtcagtgc tgagctttgc ctttctggag
     3121 agaccctccc tcgaccgggt ttcgcggttg gctaaccggc tacacttcgg tgtatcttaa
     3181 acggttcgcc cgcgcggcgc tttcgagagc cgggagccgt ccggtcggtc gaaccttgcc
     3241 cggctgccgt gccgcgactc gccggtcggc acaaaggcga cccgtcgatg gatagagttc
     3301 ttgcgcacct gaaaccgaga ggtacttaaa cagtaacgaa tggcattcaa atcactcttc
     3361 agcctgttct ccagcgacct ggcgatcgac ctcggcacgg ccaacacgct cgtctacgcc
     3421 aaggggcgcg gcatcgtcgt ctccgagcct tccatcgtcg ccatcaataa agtcaccaac
     3481 ctgcccgaag ccgtcggcaa ggaggccaag gagatgctcg ggcgcacgcc cggcaacatc
     3541 gtcgccatcc gcccgatgaa ggacggcgt
//
LOCUS       DBNGOH010000463         7403 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2269567, whole genome shotgun sequence.
ACCESSION   DBNGOH010000463 DBNGOH010000000
VERSION     DBNGOH010000463.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7403)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7403)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7403
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2269567"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(31..354)
                     /locus_tag="AC159P_09125"
     CDS             complement(31..354)
                     /locus_tag="AC159P_09125"
                     /inference="COORDINATES: protein motif:HMM:NF052368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor family protein"
                     /protein_id="HIR3695654.1"
                     /translation="MALSLYGKATHALRYWFLRRLPTCRQLVPVMSQSLERPLSLRER
                     AVMKVHLWVCIWCVRYLEQLHFMRETLRARGTHFLEDDDNPEGVCLPAEARERLKSAL
                     DRSSR"
     gene            complement(354..1001)
                     /locus_tag="AC159P_09130"
     CDS             complement(354..1001)
                     /locus_tag="AC159P_09130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006893281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR3695655.1"
                     /translation="MSREPGQVGASEGGKSQGRAAPSDPSAWVDLHGDYLYRYALLRL
                     RDPALAEDAVQETLLAALQARHTYGGRSAERTWLTGILKHKLVDHFRRASRQAYALED
                     EGEAFEHEELFRQEGKWKDHFDPARAPVEWNATPAALLERGEFWEVFDTCLSPLPARL
                     SSAFVLRELEGLSGGEVCEVLGISANNLWVMLHRARTHLRRCLELNWFKPKGERR"
     gene            complement(1091..1516)
                     /locus_tag="AC159P_09135"
     CDS             complement(1091..1516)
                     /locus_tag="AC159P_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020598619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HIR3695656.1"
                     /translation="MSLSRTRKIVSWVLQLTVAAILFQTLFFKFTGAEESKYIFSTLL
                     GDSEAVGRIGSGVVELIAVALLLYPRTAWLGALVALGTISGAIFSHLTKLGIVVKDDG
                     GLLFALALVVFAGSAAILLLRRREIPVVGRALRSQEASS"
     gene            complement(1541..3367)
                     /locus_tag="AC159P_09140"
     CDS             complement(1541..3367)
                     /locus_tag="AC159P_09140"
                     /inference="COORDINATES: protein motif:HMM:NF020893.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HIR3695657.1"
                     /translation="MKRLRKIRGPRVVLRPATMTAAASVLLLTSGHAFAQAGGASAPF
                     GLPGGADAAGFGPLALVLLLAVSTFVSEDLTCVAAGVLAAQGRVSFAVAVGGCLLGIY
                     VGDLLIFLAGRFLGRPAIRRAPLRWLVKPADVERSSAWFGRRGAAVVLLSRFLPGTRV
                     ATYFAAGVLDTGFRRFALYLFVAAAVWTPMLVGLSMILGAEVLESSLAAGQGAALKAL
                     VSGAVVFVLVKLVVRLSGFRGRRLLLSRWRRITRWEFWPPWAFYPPVVCYVLWLALEH
                     RGLTLFTCANPAIPEGGFVGESKMDILRGLSASPASRKLIARAALLPASAQPAKRLES
                     AHAFLACHGLTLPVVLKPDAGQRGSGVAVVRTEKELEEYLSRARVDTIIQEYVAGQEF
                     GVFYYRLPEEERGRIFSVTEKRFPAVTGDGRETLETLILKDERAVCMARAYFRLHGER
                     LNDVPCEGERVQLVELGTHCRGAVFLDGGRVKTEELEQAIDDLCRGFEGFYFGRFDIR
                     VPSLEEFGRGVGFKVVELNGVTSEATHIYDPATPLLAAYRTLFEQWRIAFRIGARNRA
                     RGARPTPLRTLLRLSWGHVFGSGRPRTAARTLAGNINCPSRI"
     gene            complement(3376..3918)
                     /locus_tag="AC159P_09145"
     CDS             complement(3376..3918)
                     /locus_tag="AC159P_09145"
                     /inference="COORDINATES: protein motif:HMM:NF024273.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HIR3695658.1"
                     /translation="MRDVDRLVADCVRVLEQGLALIERLDERLYAETPGLPVRSGVGE
                     HFRHCLDFYQSFLAGVASGRIDYNRRERDALVGREPAFAATKLRIVISELRRLTAEGG
                     ADVLVSLEDEAGASSEPPRWCGSTVARELQFLLSHTVHHYALVALMLRLRGFEPGEEF
                     GVASSTLKHWEREQEAVCAQ"
     gene            complement(3933..4706)
                     /locus_tag="AC159P_09150"
     CDS             complement(3933..4706)
                     /locus_tag="AC159P_09150"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013702.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HIR3695659.1"
                     /translation="MRESLVKHLRAASRLMALCCVTAGLYFSWLAAAPLALPWPDASR
                     RWRALVFRAWARAVCRIAGMRVGVRGVPPRGAFLLVSNHLGYMDVVALASVSDCVFVA
                     KSEVAGWPVLGRLCRSVNTIFVNRRRRRSLPGVIERVERALLRGSGVVVFAEGTSTGG
                     ASVAPFRSPLLDIAARKAMPVHFASLSYRTPPRCPPAREAVCWWGDMTFLKHLYGLFA
                     LPRFDAEVVFGEQPVRDGDRKALARKLWAAVSAQFVPVV"
     gene            complement(4706..5530)
                     /locus_tag="AC159P_09155"
     CDS             complement(4706..5530)
                     /locus_tag="AC159P_09155"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024836.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3695660.1"
                     /translation="MKPALLPFSTGAPEAFSYPLYAGALPPTEISKGVYVVRFARTPE
                     ELDAVLRLRFRVFNLELNEGLESSYLSGRDADEFDPTCHHLIVVEAASGEAVGTYRLR
                     TGEMADEAGGFYSEGEFDLSALPRRVREGAVEIGRACIAREHRNKQVLFLLWKGLAAY
                     MLHNRKRYFFGCCSLPTQDAREGLRALNRFQRQGHLHPTLHVPPQTGFECVPEGYFVV
                     DGQEAALPRLFHTYLRFGAKVCGPPAIDRRFKTVDFFVLFDAQGMTQHTRQLFFGA"
     gene            complement(5576..7216)
                     /locus_tag="AC159P_09160"
     CDS             complement(5576..7216)
                     /locus_tag="AC159P_09160"
                     /inference="COORDINATES: protein motif:HMM:NF019604.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR3695661.1"
                     /translation="MSKRTRKRVVILGGGFAGVYTAQQLERALGRRDDFEIVLVNKEN
                     YFVFQPMLAEVVSGSVGVTDVVSPIRRLLPRTDLHVREVESVDLRNRTVTTSPGFRPH
                     QHVLHYDHLVLALGNVTDFRGLRGLPEHALPFKNLGDALHLRNHVIHALEEAAVEKHD
                     AELRRQLLTFVVAGGGFSGVEVVAELNDFVRGVARSYPQIDPAEIRVVLLHGRERILP
                     ELTEKLGLFAQKILRRRGVEIRLNARLQAATGEEAILADGSRIPTKTLVSTIPSSPHP
                     LVEGLELPRDKSGRLMANSFLEVQGAEGVWALGDCALVPIAGGRGTSPPTAQHATRQG
                     KVAAHNIVASIRGGEKRAFSFKDLGKLGALGHRSAVAEIMGVPVSGFLAWWLWRTIYL
                     MKLPGWGRRLKVASSWTLDLLLPAEIVQLKLHNSAGITREHFEPGEAVFRQGDVGDRI
                     YIIVSGRAEVVREHAGAELALAELGAGEYFGEMALVENAARNATVRCVEPLDVIGLPK
                     REFGILAAHLPELRRSFERVVEQRQRANTLALAGRGDG"
BASE COUNT         1270 a         2545 c         2506 g         1082 t
ORIGIN      
        1 acagggagat ggcgtcggaa ggttgaaggt ctagcgggag gagcggtcga gggcactctt
       61 caggcgttcg cgggcttcgg cggggaggca gacaccttcg gggttgtcgt cgtcttcaag
      121 gaagtgcgtc ccgcgggcgc gaagagtttc gcgcatgaag tggagctgtt cgagatagcg
      181 gacgcaccag atgcagaccc acaggtgaac cttcatcacg gcgcgctcgc gcaaggagag
      241 cgggcgttcg agcgactgcg acatcacggg cacgagctgc ctgcacgtag ggagccggcg
      301 caggaaccag tagcggaggg cgtgcgtcgc cttgccgtag agtgatagag ccattatcgc
      361 cgttcgccct tcggcttgaa ccagttgagt tcgaggcagc gccgcagatg cgtcctcgcg
      421 cggtggagca tcacccagag gttgttcgcc gagatgccca agacctcaca aacctctccg
      481 ccgctcagac cctcaagctc ccgcagcacg aaggcgctgc tgaggcgcgc gggcaggggc
      541 gacaggcagg tgtcgaagac ctcccagaac tcgccgcgtt cgagcagtgc cgcgggcgtg
      601 gcgttccact cgacgggcgc gcgcgccggg tcgaagtgat ccttccactt gccctcctgt
      661 ctgaaaagct cctcgtgctc gaacgcttcg ccctcgtctt cgagggcgta agcctgcctg
      721 ctcgccctgc ggaagtggtc tacgagcttg tgcttgagga tgccggtgag ccacgtccgc
      781 tcggcgctcc gccccccgta ggtgtggcgg gcctggagcg cggcgagcag ggtctcctgc
      841 acggcgtctt cggcgagcgc cgggtctctc aggcggagca gcgcatacct gtagaggtag
      901 tcgccgtgca ggtcaaccca ggcggacggg tccgaagggg cggcgcgccc ctgagacttg
      961 ccgccctcgg acgcgccgac ctgacccggc tcgcgtgaca tcgttgagag tcgtgcgctc
     1021 accctcgacc tttagtcccg gcccgttggc ccgcagccct ccggcggact actcccttta
     1081 atgatgtgcg tcaggaactc gcctcctgag accgcagcgc gcgcccgacg acgggaatct
     1141 cgcggcggcg aagaagaagg attgcggcgc tgccggcgaa gacgacgagg gcgagcgcga
     1201 acagcagccc gccgtcgtct ttgacgacga tgccgagctt ggtcaggtgg ctgaagatgg
     1261 cgccgctgat cgtgccgagc gcgacgagcg cgccgagcca ggcggtgcgc gggtagagga
     1321 gcagcgcgac ggcgatcagc tcgaccaccc ccgagccgat gcgcccgacg gcctcgctgt
     1381 cgcccagcag agtcgagaag atgtacttcg actcctccgc gccggtgaac ttgaagaaga
     1441 gcgtctggaa cagtatggcc gcgacggtca actgcaagac ccaactgacg atttttcgtg
     1501 ttctggataa gctcatgcgt gtgtgcctcc ctttccgcag ttatatccgc gaagggcagt
     1561 taatattccc cgccagcgtg cgcgccgcgg tgcgcggtct gccgctgccg aagacgtggc
     1621 cccacgagag ccgcagcaga gttcggagcg gcgtcggcct cgcgccgcgc gcgcggttcc
     1681 gcgccccgat gcggaaggct atccgccact gctcgaagag cgtccggtag gcggcgagca
     1741 gtggcgtcgc ggggtcgtag atgtgggtcg cctcggaggt gacgccgttc agctcgacga
     1801 ctttgaagcc cacgccccgc ccgaactctt cgagcgaggg cacacggatg tcgaacctcc
     1861 cgaagtagaa cccctcgaac cctctgcaca gatcgtcgat ggcctgctcc aactcctcgg
     1921 tcttcacccg cccgccgtcg aggaagaccg cgccgcggca gtgcgtcccc agctcgacga
     1981 gctgcacgcg ctcgccctcg cagggcacgt cattcaggcg ttctccgtgg aggcggaagt
     2041 aggcgcgggc catgcagacg gcgcgctcgt ccttgaggat cagggtctcc agcgtttcgc
     2101 gcccgtcgcc ggtgacggcg gggaagcgct tctccgtgac ggagaagatt cgcccgcgct
     2161 cttcttccgg caagcggtaa taaaagaccc cgaactcctg gccggcgacg tactcctgta
     2221 tgatcgtgtc aacccgtgcg cgcgacagat actcctccag ctctttttcc gtgcgcacca
     2281 cggccacccc cgaaccgcgc tggcccgcgt ccggcttgag cacgacgggc agcgtcaggc
     2341 cgtggcaggc cagaaacgcg tgcgccgact cgagccgctt cgcgggttgc gccgacgcgg
     2401 gcagaagcgc ggcgcgcgcg atgagcttgc gagacgcggg agacgccgag agtccccgca
     2461 ggatgtccat cttcgactcg cccacgaagc cgccttcggg gatggccggg ttggcgcagg
     2521 tgaagagcgt gaggccgcgg tgttcgaggg cgagccacag gacgtagcag acgaccggcg
     2581 gataaaaggc ccacggcggc cagaactccc aacgcgtgat tcgacgccaa cgggagagca
     2641 gcagcctccg cccgcgaaaa cccgacaagc ggacgacgag cttcaccaga acaaagacga
     2701 cggcgccgct caccaacgct ttcaacgccg cgccctgccc cgccgcgagc gaggactcga
     2761 ggacttcggc ccccagaatc atcgagagac cgacgagcat cggcgtccag acggcggcgg
     2821 cgacgaagag atagagcgcg aatctccgga agcccgtgtc gaggacgccg gctgcgaagt
     2881 aggtcgcgac cctcgtcccc ggcagaaacc ggctgagcag gacgacggcg gcgcccctcc
     2941 gcccgaacca cgcggaactc ctctccacgt ccgccggctt gacgagccac ctgagcggcg
     3001 cacgcctgat cgcgggccgc cccagaaatc tcccggcgag gaagatcaga aggtctccga
     3061 cgtagatgcc gagcaggcaa ccgcctacgg ccaccgcgaa gctcacgcgc ccctgcgccg
     3121 ccagcacgcc cgcggcgacg cacgtcaggt cttcgctcac gaaggtcgaa acggcgagca
     3181 gcaagaccag cgcgagcggg ccgaacccgg cagcatccgc gccgccgggc aggccgaagg
     3241 gcgctgacgc cccacccgcc tgcgcgaagg cgtggccgga cgttaacagc agcacagagg
     3301 ccgccgcggt catggtcgcc ggtcgcagga cgacgcgcgg cccgcggatt ttccgaagac
     3361 gcttcatcgt tcccctcact gtgcgcacac agcctcctgc tcgcgctccc agtgcttgag
     3421 cgtcgaagac gcgacgccga actcctcgcc cggctcgaag ccccgcagcc gcagcatgag
     3481 cgcgacgagc gcgtaatggt ggacggtgtg gctcagcagg aactgaagct cgcgtgcgac
     3541 ggtggagccg caccagcggg gcggctcgga cgacgcgccc gcctcatctt cgaggctgac
     3601 cagcacgtcc gccccccctt cggcggtgag ccgtcgaagc tccgagatga cgatgcgcag
     3661 cttcgtggcc gcgaacgcgg gttcgcgccc gacgagcgcg tcgcgctcgc ggcggttgta
     3721 gtcgatccgg cccgaggcga cgccggcgag gaagctctgg tagaagtcca gacagtggcg
     3781 gaagtgttcg cctacgccgc tccggacggg cagccccggc gtctccgcgt agaggcgttc
     3841 gtcgagcctt tcgatcaggg ccagcccctg ttcgagcacc cgcacgcagt cggcgacaag
     3901 tcgatccacg tctctcatcc aaaacgctcc gttcagacca ccggcacgaa ttgcgcgctg
     3961 accgccgccc agagttttcg cgccagcgcc ttgcggtcgc cgtcgcgcac gggctgctcg
     4021 ccgaagacga cctcggcgtc gaaccgtggg agcgcgaaga gtccgtacag gtgtttgagg
     4081 aaggtcatgt cgccccacca gcagaccgcc tcgcgcgcgg gcgggcaccg ggggggcgtg
     4141 cggtagctga gcgacgcgaa gtggacgggc atcgctttgc gcgcggcaat gtcgaggagc
     4201 ggcgacctga acggcgcgac cgaagcgccg cccgtgctcg tcccttcggc gaagacgacg
     4261 acgcccgacc cgcgcaaaag cgcccgctcc acgcgctcta tcacgccggg gaggctgcgc
     4321 ctccggcggc ggttgacgaa gatcgtgttc acactacggc acaggcgtcc gaggacgggc
     4381 cagcccgcga cctcgctctt ggccacgaag acgcagtcgg acaccgacgc gagcgcgacc
     4441 acgtccatgt agcccaggtg gttcgagacg agtaggaacg ccccgcgcgg aggcacgccc
     4501 cgaaccccga ccctcatccc ggcgatgcga caaaccgcac gcgcccacgc gcgaaagacc
     4561 agagcgcgcc acctgcgcga ggcgtcgggc cacggcaagg cgagaggcgc ggcggcgagc
     4621 cacgagaaat acagccccgc cgttacacag cacagcgcca tgaggcgcga cgcggcccgc
     4681 aggtgtttga caagcgactc tctcatcatg caccgaagaa gagttggcgc gtgtgttggg
     4741 tcatcccctg cgcgtcgaag aggacgaaga agtccacggt cttgaaccgc cggtcaatcg
     4801 cgggcgggcc acagaccttt gcgccgaagc gcaggtatgt gtggaacagg cgcgggagcg
     4861 cggcctcctg cccgtccacg acgaagtagc cttcgggcac gcactcgaag cccgtttgcg
     4921 gcgggacgtg gagcgtcggg tgcaggtgtc cctgtcgctg gaaccggttc agcgcgcgca
     4981 ggccctcgcg cgcgtcctgc gtcgggagcg agcagcagcc gaagaagtaa cgcttgcggt
     5041 tgtggagcat gtacgcggcc agccccttcc agagcaggaa gagcacctgc ttgttccggt
     5101 gttcgcgcgc gatgcaggcc cgcccgatct cgaccgcgcc ttcccgaacc cgccgcggca
     5161 gagcggagag gtcgaactca ccctccgaat agaacccgcc cgcttcgtca gccatctcgc
     5221 ccgtccgcag ccggtaggtg ccgacggcct cgccgctcgc cgcttcgacg acgatcaggt
     5281 ggtggcacgt cgggtcgaac tcgtccgcgt cgcgccccga gaggtaagag gactccagcc
     5341 cctcgttcag ctcgaggttg aagacgcgaa agcgcagcct cagcaccgcg tccagctctt
     5401 cgggcgtgcg ggcgaaacgg acgacgtaca cgcccttcga gatttccgtc ggcggcagcg
     5461 ccccggcgta gagcgggtac gagaacgcct cgggcgcgcc ggtcgagaac ggcagcagtg
     5521 ctggtttcat caacgtcctc ccgtcgggcc aaactcttcg ggccggtgac aaaccttagc
     5581 cgtcgccccg cccggccagc gccagcgtgt tggcgcgctg gcgttgttcc accacgcgct
     5641 cgaaactccg ccgcagctcc ggcaggtgcg cggccaggat gccgaactca cgcttcggca
     5701 ggccgatgac gtccaaaggc tcgacgcacc gcaccgtcgc gttgcgcgcc gcgttctcga
     5761 cgagcgccat ctcgccgaag tactcgcccg cccccagctc cgcgagggcc aactccgccc
     5821 cggcgtgctc gcgtacgacc tcggccctgc cgctcacgat gatgtagatg cggtcgccta
     5881 cgtcgccctg gcggaagacg gcctcgcccg gctcgaagtg ctctctggtg atgcccgcgg
     5941 agttgtgcag cttgagctgc acgatctccg ccgggagcag caggtcgagc gtccaggagc
     6001 tggcgacctt cagccggcgg ccccagcccg gcagcttcat gaggtagatc gtccgccaca
     6061 gccaccacgc gaggaagccc gagacgggga cgcccatgat ctccgcgacc gccgagcggt
     6121 ggccgagcgc gccgagcttg ccgaggtctt tgaagctgaa ggcgcgcttc tcgcccccgc
     6181 ggatcgaggc gacgatgttg tgtgccgcga ccttcccctg ccgcgtggcg tgctgggcgg
     6241 tcggcgggct ggtgccgcgg ccccccgcaa tcggcacgag cgcacagtcg ccgagcgccc
     6301 agacgccctc cgcgccttgg acttccagaa aagagttagc catgagccgc cccgacttgt
     6361 cgcgcggaag ctccaggccc tcgacgagtg gatgaggcga agaggggatc gtcgagacga
     6421 gcgtcttggt cgggatgcgc gacccgtcgg cgagtatggc ctcctcgccg gtcgcggcct
     6481 gaagtcgcgc gttcaggcga atctcgacgc cgcgacggcg caagattttc tgcgcgaaga
     6541 gtccgagctt ctccgtcagc tcgggcagga tgcgctcgcg cccgtgcagc agcacgacgc
     6601 gtatctccgc cgggtcgatc tgcggatagc tgcgcgccac gccgcggacg aaatcattca
     6661 actccgctac cacttcgacg ccggaaaagc ccccgcccgc cacgacgaag gtgagcaact
     6721 ggcggcgcag ctccgcgtcg tgcttctcga ccgccgcctc ttcgagcgcg tggatgacgt
     6781 ggttgcggag gtggagcgcg tcgccgaggt tcttgaaggg aagcgcgtgt tcgggaaggc
     6841 cccgcagccc gcggaagtcc gtgacgttgc cgagcgcgag caccagatgg tcgtagtgca
     6901 ggacgtgctg gtgcgggcgg aaccccggcg aggtcgtgac cgtgcggttg cggaggtcaa
     6961 cggactcgac ctcgcggacg tgcagatccg tgcgcggcaa cagccggcgg atggggctga
     7021 ccacgtccgt gacgccgacc gagccggaga ccacttcggc cagcatcggc tggaagacga
     7081 agtagttctc cttattgacc agcacgatct cgaaatcgtc gcgccgcccc agagccctct
     7141 caagctgctg cgccgtgtag acccctgcga acccgccgcc gaggatgacc acgcgcttac
     7201 gtgtgcgttt gctcatacgt gtcacctgcc ctttagtcga ggcgggggcc ggaaacttac
     7261 aaagaatctc cggccccgta gatttttttt ttcgagtggg tgaggttcgt gtattgctac
     7321 cgcgccgccg gggcgggccg ggccgactcg cctccggccc ggccagagca caaagtctcg
     7381 gaagagggag tcggcatttg gcc
//
LOCUS       DBNGOH010000464         2088 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2819610, whole genome shotgun sequence.
ACCESSION   DBNGOH010000464 DBNGOH010000000
VERSION     DBNGOH010000464.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2088)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2819610"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            144..767
                     /gene="lexA"
                     /locus_tag="AC159P_09165"
     CDS             144..767
                     /gene="lexA"
                     /locus_tag="AC159P_09165"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HIR3695662.1"
                     /translation="MHPRTQRQKEILEYITRFIERHGYEPSYAQIARHFRVSSKATIA
                     KHIGALERQGLLARRRADGAFGLSVQHEDVDLDAVCEVPLLGRIAAGAPIDTVEDTEK
                     VTIPRFLLGSVRPNRVYALRVTGDSMIDEHICDGDIALIENRTEARDGEIVVALVEGA
                     RATLKRLFRFGPEVELRPANAQHEPLRLHASKITVQGIFRGLLRPAG"
     gene            969..2051
                     /locus_tag="AC159P_09170"
     CDS             969..2051
                     /locus_tag="AC159P_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012626070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI/YhbO family deglycase/protease"
                     /protein_id="HIR3695663.1"
                     /translation="MAETKKVAILIEQHVEDSEFQIPYAALREGGAEVTVLGSRVNEK
                     YAGKNMKVSVEADATTTEARAVDFDAVVIPGGAAPDKMRTNMKTVRFVEDAVNAGKLV
                     AAICHGPQVLIEGDLLKGRRATGFRAIRKDMQNAGANFENAPLVADGNLVTSRRPGDL
                     PIFTAAILRGLGLSVKGTHIPDEGDLAAEWWQLAEKWGGQSKDEIVDGINTAIRGERY
                     ALSAFEHYAEKATDPEIKVLFAEVVASKLRHITALEARLATLGGKEDFQAAGSGAWAT
                     LKSWLQSSNDLALLRRAMGDLQTGVVDAFNLTNQFTDPVTASVFDEIEIDLARHEQRV
                     ADLYHARLGADEAKPAQPTTGAAVSA"
BASE COUNT          395 a          718 c          682 g          293 t
ORIGIN      
        1 tcattcatca ttcatcgttc atcatttgtc ttgacgcctc gcgcgtgcgc caatagaatc
       61 acgccacaac cagagcagca gtagtcgccg cacccctccg cagcgaccta aagcggaagc
      121 cccacagggt aaggagactc gtcatgcacc cgcgcaccca gcggcagaaa gagattctcg
      181 aatacatcac gcgcttcatc gagcgccacg gctacgagcc ttcctacgcg cagatcgcgc
      241 gccacttccg cgtctcctcg aaggcaacca tcgccaagca catcggcgcg ctcgaacgac
      301 aaggcttgct cgcgcgccgc cgcgccgacg gcgccttcgg cctcagcgtc cagcacgagg
      361 acgttgacct cgacgccgtg tgcgaggtgc ctctgctcgg gcgcatcgcg gcgggcgcgc
      421 ccatcgacac cgtcgaggac acggagaagg tcacgatccc gcgcttcctg ctgggcagtg
      481 tgcgcccgaa ccgcgtctac gctttgcgcg tgacgggcga ttcgatgatc gacgagcaca
      541 tctgcgacgg cgacatcgcg ctcatcgaga accgcaccga ggcgcgcgac ggcgagatcg
      601 tcgtcgccct cgtcgaaggc gcgcgcgcca ccctgaagcg cctcttccgc ttcggccccg
      661 aggtcgaact caggcctgcc aacgcgcagc acgagccgct ccgcctccac gcctcgaaga
      721 tcacggtgca gggcatcttc cgcggcctcc tgcgcccggc gggctgatgc cccgcccggc
      781 ggcggcgcga ccggcactcg cttaaaaagt aacggaagca gacagaaccg ctcgcggcct
      841 gtacgggttt gcgggcggtt ctcgtgcgtg cggcgccatt tcctccggcc ttcgcgctgg
      901 catgggcctt gcccggttga tatggaagcg aacaattcat caaccagcaa gtcgaaggag
      961 tgtgcattat ggcagagacg aaaaaggtcg ccatcctcat cgagcagcac gtcgaggata
     1021 gcgagttcca gattccgtac gcggcgctca gggagggggg cgcggaagtg accgtgctcg
     1081 gctcgcgcgt caacgagaag tacgcgggca agaacatgaa ggtctcggtc gaggccgacg
     1141 cgacgaccac cgaggcgcgc gcagtggact tcgacgccgt cgtcatcccc ggcggcgcgg
     1201 cgcccgacaa gatgcggacg aacatgaaga ccgtgcgctt cgtcgaggac gcggtgaacg
     1261 ccggcaagct cgtcgccgcc atctgccacg ggccgcaggt gttgatcgaa ggcgacctct
     1321 tgaagggtcg ccgcgcgacg ggcttccgcg ccatccgcaa ggacatgcag aacgcgggcg
     1381 cgaacttcga gaacgcgccg ctcgtcgccg acggcaacct cgtcacgtcg cgccgaccgg
     1441 gcgatttacc gatcttcacg gcggccatcc tgcgcggcct cggcctgagc gtgaagggga
     1501 cgcacatccc cgacgagggc gatctggcgg cggagtggtg gcagctcgcc gagaagtggg
     1561 gcgggcagtc gaaggacgag atcgtggacg gcatcaacac ggccatccgc ggcgagcgct
     1621 acgcgctgtc ggccttcgag cactacgccg agaaggcgac cgaccccgag atcaaagtgc
     1681 tcttcgcgga ggtcgtcgcc agcaagctgc gacacatcac cgcgctcgaa gcgcggctcg
     1741 cgacgctcgg cggcaaagag gactttcagg cggcgggcag cggcgcgtgg gcgacgctga
     1801 agagctggct gcaatcgtct aacgacctcg cgctcctgcg ccgcgcgatg ggcgacttgc
     1861 agaccggcgt ggtggacgcc ttcaacctca cgaaccagtt caccgacccc gtcaccgcct
     1921 ccgtcttcga cgagatcgaa atcgacctcg cgcgccacga gcagcgcgtc gcagacctct
     1981 accacgcgcg cctcggcgcc gacgaggcca aaccggcgca gccgacgacg ggcgccgccg
     2041 tctccgcctg aaaagcagta ggggagaaag ggagaagggg agagcggg
//
LOCUS       DBNGOH010000465         5860 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3920664, whole genome shotgun sequence.
ACCESSION   DBNGOH010000465 DBNGOH010000000
VERSION     DBNGOH010000465.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5860)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5860)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5860
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3920664"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            122..409
                     /locus_tag="AC159P_09175"
     CDS             122..409
                     /locus_tag="AC159P_09175"
                     /inference="COORDINATES: protein motif:HMM:NF022659.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3006 domain-containing protein"
                     /protein_id="HIR3695664.1"
                     /translation="MPDEEKEGGSKRSRGVIDRVEDGGTAVVMFGEDEALKVELPAAL
                     LPDGASDGDHLTLTVRLEAASRKEAEQSVKALQEKLEKRGGDRGEQKSFKL"
     gene            445..693
                     /locus_tag="AC159P_09180"
     CDS             445..693
                     /locus_tag="AC159P_09180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HIR3695665.1"
                     /translation="MGLDTVELIMAFEERFGIRIPDAEAPELYTPRRVTDYIMKHYGH
                     RGLERAQVAAIVRAVIEQETAVYDFDEDSHFVLDMGLD"
     gene            742..1173
                     /locus_tag="AC159P_09185"
     CDS             742..1173
                     /locus_tag="AC159P_09185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695666.1"
                     /translation="MKLSDTLNVVLRQCRADGVPMRPPRPEGEVRAMLQATGRKVSRD
                     VIELYCATDGMEDGGMDSHCWSLWPLEKLLAENAAHSRPHILFSDFLIHSYAYCFDWR
                     SEDESSVRLELWDGNEPRQVAGSLHRFFELYLTRPEALELF"
     gene            1176..2207
                     /gene="purM"
                     /locus_tag="AC159P_09190"
     CDS             1176..2207
                     /gene="purM"
                     /locus_tag="AC159P_09190"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HIR3695667.1"
                     /translation="MTKSIPYSDAGVSIDRANAAVEQIKRLARGTFGPRTLSEIGSFG
                     GMFDGAFPALKAPVLVASADGVGTKLKIAFMTGRHDTVGRDLVNHCVNDILVQGARPL
                     FFLDYVATGVLAPEVIVSIVEGVARGCRENGCALLGGETAEMPGFYAAGEYDVAGFIV
                     GIVEREKVIDGSRIAPGDVLLALPSAGLHTNGYSLARKLFFETAGHTPDTRLEALGTT
                     VGEALLAPHLSYLRPLEGLLDTGLIKGLAHITGGGLLDNIPRILPEGAAVEINRGSWP
                     VPAVFRVMQEIGNVSEEEMHRTFNMGLGMVVVCAPSDAETIRYHFDSPGECHRIGRVV
                     AGARTVTLV"
     gene            2426..5350
                     /locus_tag="AC159P_09195"
     CDS             2426..5350
                     /locus_tag="AC159P_09195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695668.1"
                     /translation="MERLSPPPEDDGSRIPPVEKRIYEKPVAWLLGRQLLNSFKGALL
                     YSALGSKLDARDWMAAEVLDFADDGGEDEFWFDYLSDTGDGVMAVYSLAYLSMSDLWV
                     KSPWSQIAEMKDGDDRVKLARDEGFASKLPRGRFLFVGGDTAYHASDYSTLAARFQLP
                     FRWAFADLFGAERGKEPPFCRPLVGIPANHDYYDQLDGYRRQFRAPVREEKCGRLYVN
                     PADPETAPQLMLPGHRRVQKASYVALKLPFGWWMWGLDTDVGEVDERQRKFFREIEVE
                     RGRSVAEGATPDKLVVATCAPTTVFGKYADAAADTKAARAFWQVGLARPFLRDEELDK
                     EQEDLDKGQQEPRLGDDQLRLDLSGDIHLYARYWGPPSGRRPRERAAAEQPRADNYAS
                     VVSGTGGAFHHPSQTYLDEIQEQALYPPVTESRGAVAKEIFKPWKLMNGGGVWLVGFI
                     AAFVIYFAATTVPSSRDVLNNFHLDNFLVRDFNLWHAVGFAPQTEEGRPLHAKVCPTL
                     PTRDAPREIYPFKLWTTLLGVPTTRTLAETRTAACNQLPNCTTPTGQTGAQPAYFWGA
                     CAVSDTPDDYTLGVVLALLSLVPVALGFAAHGWLLQDPDLVKTFTMWKVAKGAHARPW
                     YKERPFVRSFFFVVLPLVLLSALALFTIRPYRAHITPFGNSMFVLLSLVWAGSAVVLN
                     LRYSDWLFHKTMVRKLNRWDWLLTWVLAVYAVLAVGAGVWFFGRNNQMAYVLTDNLFA
                     VVALIIFFGLTFLGYSKAGENAKGIAGKLPFLLLGALHGAAQMLTPFVLVRKGSWLTL
                     AAAVVLVVAFALLGRELLKRDMRWPLALAGLVYAALMVSLPCVTARLFGSPASYAASS
                     GLFAHDYHDGWSGLLPAIVSGLVAVVTSCVWLGWYLAVALSFGGHNNEAGGAARIERF
                     KEFVRVRLARDPATGEDTLTAYVIAVDEPKKKGAELQPRLADVFTLRRKTAAASPEGR
                     RA"
     gene            5449..>5860
                     /locus_tag="AC159P_09200"
     CDS             5449..>5860
                     /locus_tag="AC159P_09200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695669.1"
                     /translation="MSSYTPPPKLERPVRYVGLANRPQGLRLPPARLPIMMDWKLRKR
                     WRYVAVWTRDITVYGASAWTGPVAQEFWAVLDRRDEKNVKVHERTRNIIPGKVVLTPG
                     RMYVKDQTKNGDRIEFDVALTEDEGFEVVTPSGRA"
BASE COUNT          958 a         2009 c         1915 g          978 t
ORIGIN      
        1 aaaccgtaaa ccgtgacaag tgaagaaccg gttttagcct ttcactcttc acacttcact
       61 cgtcactgca ttcttctcat cacggttcac ggttcacggt tcacggcctt tgaggtgtgc
      121 gatgccagac gaagagaaag agggcggctc gaagcgttcg cgcggcgtca tcgaccgcgt
      181 cgaggacggc gggacggcgg tcgtcatgtt cggcgaagac gaggcgttga aggtcgagct
      241 gcccgcggcg ctgctccccg atggcgcttc ggacggcgac catctgacgc tgaccgtcag
      301 gctcgaagcc gcgtcgcgca aggaggccga gcagagcgtc aaggcgctgc aagagaagct
      361 ggagaagcgc ggcggcgacc gcggcgagca gaagagcttc aagctgtaat gccgccgcgc
      421 ccgcgttcgc ttcgcggtgt gacgatgggc ctcgacacgg tcgaactcat catggccttc
      481 gaggagcggt tcggcatccg gattcccgac gccgaagcgc cggagctgta cacgccgcgc
      541 cgcgtgaccg actacatcat gaagcattac ggtcataggg gtttggagag ggcgcaggtg
      601 gccgccatcg tgcgcgcggt catcgagcag gagacggcag tttacgactt cgacgaggac
      661 tcgcacttcg tcctcgacat gggcctcgat tgagtgtggc ccgccgcgga gggaggccgc
      721 cgcgcgtccc ggttgaaacg gatgaaactc tcggacactt tgaacgtggt gctccggcaa
      781 tgccgcgctg acggcgtgcc gatgcgcccg ccgaggccgg agggcgaagt gcgggccatg
      841 ttgcaagcga ctggccggaa agtgtctcgt gatgtcatcg agctttattg cgcgaccgac
      901 gggatggagg acggcgggat ggacagccac tgctggtcgc tctggccgct ggaaaagctc
      961 ttggccgaga acgcggctca ttcccgtccc cacattctgt tctccgattt tctgattcac
     1021 tcttacgcct attgcttcga ctggcggagt gaagacgaat cgtcagtccg ccttgagttg
     1081 tgggacggca acgagccgcg gcaagtggcg ggcagcctgc accggttctt cgagctgtat
     1141 ttgacacgac ctgaggcgct ggaacttttc tgaagatgac gaaatccatc ccctattcgg
     1201 acgcgggcgt ctcgatcgac cgcgcgaacg cggcggtcga gcagatcaag cggctcgcgc
     1261 gcggcacctt cggcccgcgc acgctctccg agatcggcag ctttggcggg atgttcgacg
     1321 gcgcgttccc tgcgttgaag gcgccggtgc tcgtcgcctc ggcggacggc gtcgggacga
     1381 agttgaagat cgccttcatg accggcaggc acgacaccgt gggccgcgac ctcgtcaacc
     1441 actgcgtcaa cgacatcctc gtgcagggcg cgcgcccgct cttcttcctc gactacgtgg
     1501 cgaccggcgt gctcgcgccc gaggtgatcg tgtccatcgt cgaaggcgtc gcgcgcgggt
     1561 gccgcgagaa cgggtgcgct ttgctcggcg gcgagacggc ggagatgccc ggcttctacg
     1621 ccgcgggcga gtacgacgtg gcgggcttca tcgtcggcat cgtcgaacgc gagaaggtca
     1681 tcgacggcag ccgcatcgcg ccgggcgacg tgctgctcgc gcttccctct gccgggctgc
     1741 acacgaacgg ctactcgctc gcgcgcaagc tcttcttcga gacggcgggc cacacgcccg
     1801 acacccgtct cgaagcgctc ggcacgacgg tcggcgaagc gctgctcgca ccgcacctca
     1861 gctacctccg cccgctcgaa ggcttgctcg acacggggtt gattaaaggt ctcgcgcaca
     1921 tcacgggcgg cggcctcttg gacaacatcc cgcgcatcct gcccgaaggc gccgccgtcg
     1981 aaatcaatcg cggctcgtgg cccgtgcccg ccgtcttccg cgtcatgcaa gagatcggca
     2041 acgtctccga agaggagatg caccgcacct tcaacatggg cctcgggatg gtcgtcgtct
     2101 gcgctccttc agacgcggag accatccgtt atcacttcga cagccccggc gagtgccacc
     2161 gcatcgggcg cgtcgtcgcg ggcgcgcgca cggtcacgct ggtttaagtc gcgcatgata
     2221 gttgtagggg cagggcttgt ccctgcccgc gtttacatct cattcaacgt cgaatcaatc
     2281 tgccgcggcg ggcagggaca agccctgccc ctacagtcat cacgaacgtc ctgcaatcga
     2341 ctgaagcttg ctctatttcc aagcttcacg cggccacgcc tcgcgccgtt cgcctcaacc
     2401 gcccctcgcc gaaaggatga agcacatgga gcgactctcc cccccgccgg aagacgacgg
     2461 ctcgcgcatc ccgcccgtcg aaaaacgaat ctacgagaaa ccggtcgcgt ggctgctcgg
     2521 gcggcaactg ctcaacagct tcaagggcgc gctcctctac tccgcgctcg gcagcaagct
     2581 cgacgcgcgc gactggatgg ccgccgaagt cctggacttc gcggacgacg gcggcgaaga
     2641 cgagttctgg ttcgactatc tttcagacac cggcgacggc gtgatggccg tctacagcct
     2701 cgcttatctt tcgatgagcg acctctgggt aaagagtccg tggtcgcaga ttgcggagat
     2761 gaaggacggc gacgaccggg tcaagctcgc gcgtgatgaa ggcttcgcgt cgaagctgcc
     2821 gcgcgggcgc ttcctcttcg tcggcggcga cacggcctat cacgcctccg actactcgac
     2881 gctcgccgcg cgcttccagc tccccttccg ctgggccttc gcagacctct tcggcgcgga
     2941 gcgcggcaag gagccgccct tctgccgccc gctcgtcggc atccccgcca accacgacta
     3001 ctacgaccag ctcgacggct accgccgaca gttccgcgcg cccgtccgcg aagagaagtg
     3061 cgggcggctc tacgtcaacc ccgccgaccc cgagaccgcg ccgcagctca tgctgcccgg
     3121 ccaccgccgc gtgcagaagg cgagctacgt cgccttgaag ctccccttcg gctggtggat
     3181 gtggggactc gacaccgacg tgggcgaggt ggacgagcgg cagcgcaagt tcttccgcga
     3241 gatcgaagtc gagcgcgggc ggagcgtcgc cgaaggcgcg acgcccgaca agctcgtcgt
     3301 cgccacctgc gcgccgacca ccgtcttcgg caagtacgcc gacgccgcgg cggacacgaa
     3361 ggccgcgcgc gccttctggc aagtcggcct cgcgcgcccc ttcctgcgcg acgaagaact
     3421 ggacaaggag caggaagacc tcgacaaggg gcagcaggag ccgaggctcg gcgacgacca
     3481 attgcgcctc gacctgtcgg gcgacataca cctctacgcg cgctactggg ggccgccctc
     3541 tggacggcgc ccgcgcgagc gtgccgcggc ggagcagccg cgggcggaca actacgcttc
     3601 ggtcgtctcc ggcaccggcg gcgcgttcca tcacccttcg cagacctacc tcgacgagat
     3661 acaggagcag gcgctctacc cgccggtcac ggagtcgcgc ggggcggtcg ccaaagaaat
     3721 cttcaagccg tggaagctga tgaacggcgg cggcgtctgg ctcgtcggct tcatcgccgc
     3781 cttcgtcatc tacttcgcgg cgaccaccgt cccttcgagc cgcgacgtgc tcaacaactt
     3841 ccacctcgac aacttcctcg tccgtgattt caacctctgg cacgccgtcg gcttcgcgcc
     3901 gcagaccgag gaaggccgac cgctccacgc gaaagtctgc ccgacgctgc cgacccgaga
     3961 cgcgccccgc gagatttatc ccttcaagct ctggacgacg ctcctcggcg tgccgacgac
     4021 gcgcacgctc gccgagacgc gcaccgccgc ctgcaaccaa ctgccgaact gcacgactcc
     4081 gaccggccag accggcgcgc agcccgcgta cttctggggg gcgtgcgccg tctcggacac
     4141 gcccgacgac tacacgctcg gcgtcgtgct cgcgctgctc tcgctcgtcc ccgtcgcgct
     4201 cggcttcgcc gcgcacggct ggctcctgca agaccccgac ctcgtcaaga ccttcacgat
     4261 gtggaaggtt gcgaagggcg cgcacgcacg cccctggtac aaagagcgac cgttcgtcag
     4321 gagcttcttc ttcgtggtgc tgccgctggt gttgctgtcg gcgctcgcgc tcttcaccat
     4381 ccgcccttat cgcgcgcaca tcacgccgtt cggcaacagc atgttcgtgc tgctctcgct
     4441 cgtgtgggcc gggtcggcgg tggtgctcaa cctgcgctac agcgactggc tcttccacaa
     4501 gacgatggtg cggaagctca accgctggga ctggctgctg acgtgggtgc tcgcggtcta
     4561 cgcggtgctc gcggtcggcg cgggcgtctg gttcttcggc aggaacaacc agatggccta
     4621 cgtgctgacg gacaacctct tcgccgtcgt cgcgttgatt atcttcttcg gcctcacctt
     4681 cctcggctac tcgaaggcgg gcgagaacgc gaagggcatc gcgggcaagc tgccgttcct
     4741 actgctcggc gcgctgcacg gcgcggcgca gatgctcacg cccttcgtgc tcgtccgtaa
     4801 agggtcttgg ctgacgctcg cggcggcggt cgtgctcgtc gtcgccttcg cgctgttggg
     4861 ccgcgagttg ttgaagcggg acatgaggtg gccgctggcg ctggcgggcc tcgtgtacgc
     4921 cgcgctgatg gtctcgctgc cgtgcgtgac ggcgcgcctc ttcggctcgc ccgcctcgta
     4981 cgcggcgtcg agcgggctgt tcgcgcacga ctaccacgac ggctggtcgg ggctgctgcc
     5041 cgcaatcgtc tcgggcctcg tggcggtggt gacgagctgc gtctggctcg gctggtatct
     5101 ggccgtcgcg ctctcgttcg gcggccacaa caacgaggcc ggcggcgccg cgcgcatcga
     5161 gcgcttcaag gagttcgtcc gcgtccgcct cgcgcgcgac cccgcgaccg gcgaagacac
     5221 gctgaccgcc tacgtcatcg ccgtggacga gccgaagaag aagggcgcgg agcttcagcc
     5281 gcgcctcgcg gacgtgttca cgctgcggag gaagaccgcc gccgcttcgc cggagggacg
     5341 ccgcgcgtga ggcgttgacg agcgcgcgta aggatggaag acgttaggag tgaagacgcg
     5401 cgccgcgtgc ctcacacgcg ccgcgcgtca cgaaaggagt atcgacagat gagtagctac
     5461 acaccgcctc ccaagctcga acggcccgtc cgctacgtcg gcctcgccaa ccgcccgcag
     5521 ggcctgcggc tcccgcccgc gcgcctgccg atcatgatgg actggaagct ccgcaagcgc
     5581 tggcgctacg tcgccgtctg gacgcgcgac atcaccgtct acggcgcgag cgcgtggacc
     5641 ggccccgtcg cgcaggagtt ctgggccgtg ctggatcgca gggacgagaa gaacgtcaaa
     5701 gtccacgagc gcacgcgcaa catcatcccc ggcaaggtcg tgctgacgcc gggccgcatg
     5761 tacgtcaaag accaaacgaa gaacggcgac cggatcgagt tcgacgtggc gctcacggag
     5821 gacgagggct tcgaggtcgt cacgccctcg ggccgcgcct
//
LOCUS       DBNGOH010000466         5717 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_253406, whole genome shotgun sequence.
ACCESSION   DBNGOH010000466 DBNGOH010000000
VERSION     DBNGOH010000466.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5717)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5717)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5717
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_253406"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..594
                     /locus_tag="AC159P_09205"
     CDS             <1..594
                     /locus_tag="AC159P_09205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HIR3695670.1"
                     /translation="LARAQAEGGIDLGAILGGGGSDGSPRGGTTPTTVPSEERGGGIN
                     GLPFPTVSISRPRTDRRDPRDPRYPPDPRDPVGRDASGAPMPGQRDEGGRSSDPTRED
                     SIDRLLDTLYKTADGYLEDIARVSGGRLVRADTLASLPAAFKEIAAELRTQYSIGYYP
                     TTPVTKGGYRKIKVRTTRKGAVVRARPGYTAVKDFRR"
     gene            597..1418
                     /locus_tag="AC159P_09210"
     CDS             597..1418
                     /locus_tag="AC159P_09210"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012958.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HIR3695671.1"
                     /translation="MFRVILAAVTLAAAALSHTGCRADGARVSADGDAAAQQRAPTGA
                     DAAFQRKTHVGARGERMRYLLFEPADYDRRRKYPLVLWLHGGGSRGEDLDLILAHGDK
                     HGPLFLARPDNQKNYPAFVVAPQCPAGRFWSDTDSDQPSAEMRLVLELLDRLRAEHSI
                     DERRLYALGISMGGYAAWDIITRRPGMFAAAVPICGGGSESRASLVTGTAVWAFHGDR
                     DEAVNVSESRRMIAALRKAGAATRYTEYEGVGHNSWERAFAEPDLLPWLFAQRRK"
     gene            1540..1662
                     /locus_tag="AC159P_09215"
     CDS             1540..1662
                     /locus_tag="AC159P_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695672.1"
                     /translation="MEGPLRGIIAGVAAWKLGGGCISTILIFIVVFWALGYVNC"
     gene            complement(1850..4024)
                     /gene="glgP"
                     /locus_tag="AC159P_09220"
     CDS             complement(1850..4024)
                     /gene="glgP"
                     /locus_tag="AC159P_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012627497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004645 - 1,4-alpha-oligoglucan
                     phosphorylase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HIR3695673.1"
                     /translation="MVQAMKEIEPTVGRTTTKGALPETISALERLSWNYWWSWATGGN
                     SVFRDLDPDVWNECEHNPRRLLQDVSEYTLARMATDPHYIERVRRLEENFDAYMNPGV
                     RTWAAQHAPEIRHERPVAYFCAEFGVHHSLPLYSGGLGILAGDHLKSASDLGLPLVAV
                     GLLYHHGYFRQRLRRDGWQEETYHQIDVDDLPLQLVRDAEGEPVNVTLSMRGREVRVQ
                     AWRVDVGRIPLYLLDTNVEGNHEIDRMITGHLYGGDRETRCVQEMVLGIGGVRLLRQL
                     NIEPHVFHLNEGHSAFLTLELARELTAEGKQFEEAAAEVRRRCAFTTHTPVAAGHDEF
                     VPPLVEACFGEDYWQALGLTLEEFLNLGRVKADNDEELFGLTPLALRMTRSTNGVSRK
                     HGEVSRELWREMWPGRTVEEVPITSVTNGVHAPTWVAPLIRSIYERHVGPDWDEALYD
                     AESWAVKVDAIPDEELWKAKRLLKHRLFAFVRQRLYFARTQQGESREYAEAAHQLFDS
                     DALTIGFARRVAAYKRWDLMLEDPERLRRLMQDAERPVQFVFAGKAHPQDQGAKLILQ
                     QLALWKLDPQVMQRAVFLQDYDQEIARNLVQSVDVWLNVPRRPLEASGTSGEKVAMNG
                     GLNLSVLDGWWPEGYDGTNGWAVGDSTIAESADEQDVEDSESLYRVLEAEVVPTFYER
                     DERGVPRRWVAMMKRAIQTLAPAFNSDRMVQDYTRKIYIGGE"
     gene            complement(4047..4589)
                     /locus_tag="AC159P_09225"
     CDS             complement(4047..4589)
                     /locus_tag="AC159P_09225"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HIR3695674.1"
                     /translation="MDFLGGRVRVVIGDITVQEVDALVNAANSSLLGGGGVDGAIHAA
                     GGPAILAECRELRRARYPEGLPTGEAVITTGGLLAARRVIHAVGPVYGHEGGREAELL
                     AACYRNSLALALEHGLKTLAFPSVSTGAFGYPRDEAAAVASAAVEDYLKGVDFISEVR
                     LVFFSQKDAESFLRHHEFGS"
     gene            complement(4732..5100)
                     /locus_tag="AC159P_09230"
     CDS             complement(4732..5100)
                     /locus_tag="AC159P_09230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695675.1"
                     /translation="MKDKKPDEYCLIVEGSYLTESEAERALRDPFIEDWVEQTGRFRL
                     HNLGEMVVVPGVMLGTLGVLMLDDGVFEIASTDTEHPLTEHKARGVAEALRRYDMFEE
                     ISVEPRDGLAGDESPLEDDD"
     gene            complement(5313..>5717)
                     /locus_tag="AC159P_09235"
     CDS             complement(5313..>5717)
                     /locus_tag="AC159P_09235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HIR3695676.1"
                     /translation="GSTAGSPYNPYVEVRDALKRFPAGGTNRNALLLVSDGLDTSRGF
                     DFTSSVNSIDLDRAISEAKKRDVVVYSFYAPSVGLTSSSRLAVSFGQSALNRLSNETG
                     GRAFFQGTDYVTFDAYFKRLSKTINEQYGRAY"
BASE COUNT          892 a         1995 c         1888 g          942 t
ORIGIN      
        1 ctcgcgcgcg cgcaggccga aggcggcatc gacctcggcg cgatcctcgg cggcggcggc
       61 agcgacggct cgccgcgcgg cggcaccacg cccaccaccg tccccagcga agagaggggc
      121 ggcggcatca acggcctccc cttcccgacc gtctccatct caaggccgcg cacggatcgg
      181 cgcgacccgc gcgacccgcg ctatccgccc gacccgcgcg accccgtcgg gcgcgacgcc
      241 tcgggagcgc cgatgcccgg ccagcgcgac gaaggcggtc ggtcgtccga ccccacgcgc
      301 gaagactcca tcgacaggct gctcgacacg ctctacaaga cggcggacgg ctacctcgaa
      361 gacatcgcca gagtctcggg cgggcgactc gtccgcgcgg acacgctcgc ctcgctgccc
      421 gcggccttca aagagatcgc cgccgagttg cggacgcaat actccatcgg ctactacccg
      481 acgacgcccg tcaccaaagg cggctaccgc aagatcaagg tgcgcaccac ccgcaaaggc
      541 gccgtcgtcc gcgcgcgccc cggctatacc gcggtcaaag acttcaggcg atagatttgt
      601 tccgcgtcat tctcgccgcc gtgacactcg ccgccgccgc gctctctcac acgggctgcc
      661 gcgccgacgg cgcgagggtg agcgccgacg gcgacgcggc tgcgcagcag cgggcgccga
      721 cgggcgccga cgcagcgttc cagcgtaaga cgcatgtcgg ggcgcgcggc gagaggatgc
      781 gctacctgct cttcgagcct gcggattacg accggcgccg gaagtacccg ctcgtgctct
      841 ggctccacgg cggcggttcg cgcggcgaag acctcgacct catcctcgcg cacggcgaca
      901 agcacggccc gctctttctg gcgaggcccg acaaccagaa gaactatccc gccttcgtcg
      961 tcgcgccgca gtgccccgcc ggacggttct ggtctgacac tgactccgac cagccttccg
     1021 cggagatgcg cctcgtgctt gagttgctcg acaggctgcg tgccgagcac agcatcgacg
     1081 agcggcgcct ctacgcgctc ggcatctcga tgggcggcta cgcggcgtgg gatatcatca
     1141 cgcgccggcc cggcatgttc gcggcggcgg tgcccatctg cggcggcggc tccgagtcgc
     1201 gcgcgagcct cgtgacgggc accgccgtct gggccttcca cggcgacagg gacgaggcgg
     1261 tcaacgtctc ggaatcgcgc cgcatgatcg ccgcgctgag gaaggccggc gccgcgacgc
     1321 gctacacgga gtacgagggc gtcggccaca actcgtggga acgcgccttc gccgagcctg
     1381 acctgctgcc ctggctcttc gcgcagcggc ggaagtaggc cgggcgcggg cgggccggag
     1441 ggtctattga cttccccggc gatttcccgt agaagttgca ccgacacccc ccgtagaaaa
     1501 ttcccccgga cgagaccgaa agaacgagga ggatttttta tggaaggccc tttgcgcgga
     1561 atcattgcgg gcgtcgcagc atggaagctc ggcgggggtt gtatctcgac gattcttatc
     1621 tttatcgtcg tcttctgggc gctcggctac gtgaactgct gaagaaagta ggcagtaggc
     1681 ggtaggcagt aggcagtcaa agcagaaaga ggaagaggct tgagacgttg aatctcaagc
     1741 ctcttcctct ttctgctttg acgaacgatg aggcttggca ctttatctct caagcctctt
     1801 gccttttctg cctactgcct actgcctact gcctactgtt tgttaccgcc tactctcctc
     1861 cgatgtaaat cttgcgcgtg tagtcctgca ccatgcggtc gctgttgaag gcgggggcga
     1921 gcgtctggat ggcgcgcttc atcatcgcca cccagcggcg cggcaccccg cgctcgtcgc
     1981 gctcgtagaa ggtcggcacg acctccgctt cgaggacgcg gtagagcgat tcggagtcct
     2041 ccacgtcctg ctcgtcggcg ctctcggcga tggtcgagtc gcccaccgcc cagccgttcg
     2101 tgccgtcgta gccctcgggc caccacccgt ccagcaccga gaggttgagg ccgccgttca
     2161 tcgccacctt ctcgccgctc gtgcccgagg cttcgagcgg gcggcgcggg acgttgagcc
     2221 acacgtccac cgactgcacg aggttgcgcg cgatctcctg gtcgtagtcc tgcaagaaga
     2281 cggcgcgctg catcacctgc gggtcgagct tccacagggc gagctgctgg aggatgagct
     2341 tcgcgccctg atcctgcggg tgggccttgc ccgcgaagac gaactggacg gggcgctcgg
     2401 cgtcctgcat caggcggcgc aggcgctccg ggtcttcgag catcaggtcc cagcgcttgt
     2461 aggccgcgac gcggcgcgcg aacccgatgg tcagcgcgtc ggaatcgaag agctggtgcg
     2521 ccgcctcggc atactcccgc gactcgccct gctgcgtgcg cgcgaagtag aggcgctggc
     2581 gcacgaaggc gaagagacgg tgcttcagca gccgcttcgc cttccagagt tcctcgtccg
     2641 ggatcgcgtc caccttgacc gcccacgact cggcgtcgta cagcgcctcg tcccagtccg
     2701 ggccgacgtg ccgctcgtag atcgagcgga tcaagggcgc cacccacgtc ggcgcgtgca
     2761 cgccgttcgt gaccgaagtg atcggcacct cctcgaccgt cctgcccggc cacatctccc
     2821 gccacagctc gcgcgacacc tcgccgtgct tgcggctgac gccgttcgtc gagcgcgtca
     2881 tccgcagcgc gagcggggtc agcccgaaca gctcctcgtc gttgtccgcc ttcacgcgcc
     2941 cgaggttcag gaactcttcg agcgtgaggc cgagcgcctg ccagtagtcc tcgccgaagc
     3001 acgcctcgac gagcggcggg acgaactcgt cgtgcccggc ggcgaccggc gtgtgcgtcg
     3061 tgaacgcgca gcggcggcgc acctcggcgg cggcctcctc gaactgcttg ccttccgccg
     3121 tcagctcgcg cgccagctcc agcgtgagga aggccgagtg gccttcgttg aggtggaaga
     3181 cgtgcggctc gatgttcagt tgacgcagga ggcgcacgcc gccgatccct aagaccatct
     3241 cctgcacgca ccgcgtctcg cggtcgccgc cgtagaggtg gcccgtgatc atccggtcga
     3301 tctcgtggtt gccctcgacg ttcgtgtcca gcaggtagag cgggatgcgc cccacgtcca
     3361 cgcgccacgc ctgcacgcgc acctcgcgcc cgcgcatcga gagcgtgacg ttgacgggtt
     3421 cgccctccgc gtcgcgcacg agctggagcg gcaggtcgtc cacgtcgatc tggtggtagg
     3481 tctcctcctg ccagccgtcg cggcgcagcc gttgacggaa gtagccgtgg tgatagagca
     3541 gcccgacggc gacgagcggc agccccaggt cgctcgccga cttcaggtgg tcgcccgcga
     3601 ggatgccgag gccgcccgag tagagcggca gcgagtggtg gacgccgaac tcggcgcaga
     3661 agtaggcgac cgggcgctcg tgcctgatct cgggcgcgtg ctgcgccgcc cacgtgcgca
     3721 cgcccggatt catgtaggcg tcgaagttct cttcgagccg ccgcacgcgc tcgatatagt
     3781 gcgggtcggt cgccatccgc gccagagtgt actcggagac atcttgcagc agccgccgcg
     3841 ggttgtgctc gcactcgttc cacacgtccg ggtcgaggtc gcggaagacg gagttgccgc
     3901 ccgtcgccca gctccaccag tagttccacg aaaggcgctc caaggcgctg atcgtctcgg
     3961 gcagcgcgcc cttcgtcgtc gttcgtccga ctgtcggctc gatctccttc atcgcttgaa
     4021 ccatcgtttc cttttctttc ttcccgtcaa ctcccgaatt catgatggcg taaaaaactc
     4081 tccgcgtcct tctgcgagaa gaagaccagc ctcacctcgc tgataaagtc aacacccttc
     4141 agataatcct cgaccgcggc ggaggcgacg gcggcggcct cgtcgcgcgg gtatccgaac
     4201 gcgcccgtcg agaccgacgg gaaggccaac gtcttcagcc cgtgctcgag cgcgagcgcg
     4261 agcgagttgc ggtagcacgc cgcgagcagc tccgcctcgc gcccgccctc gtgcccgtag
     4321 acggggccga ccgcgtggat gacgcggcgc gccgcgagaa gcccgcccgt cgtgatcacc
     4381 gcctcgcccg tcggcagccc ttccgggtag cgcgcgcgcc gcagctcgcg gcactccgcg
     4441 agaatcgcgg ggccgcccgc cgcgtgaatc gcgccgtcca cgccgccccc gccgagcaag
     4501 ctggagttgg ccgcgttgac cagcgcgtca acctcctgca cggtgatatc tccgataacg
     4561 acgcggacgc gccctccaag aaaatccatc ttcgtttgtt ggggcaacga atctaccact
     4621 aaagtgcgcg ccaatgcacg ccgcgaggcg ccgcgcgcac gagactcttc ggatcaggaa
     4681 gaggggaacg taagggattt cgggaaatct gtcttggggg attcggcggc ctcagtcgtc
     4741 gtcttcgagc ggggattcgt ccccggccaa cccgtcgcgc ggctcgacgc tgatctcctc
     4801 gaacatgtcg tagcggcgga gcgcctcggc cacgccgcgc gccttgtgtt cggtgagcgg
     4861 gtgctcggtg tcggtgctcg cgatctcgaa cacgccgtcg tcgagcatca ggacgccgag
     4921 cgtgccgagc atgacgccgg gaacgacgac catctcgccg aggttgtgaa ggcggaagcg
     4981 cccggtctgc tcgacccagt cttcgatgaa ggggtcgcgc agcgcgcgct ccgcctccga
     5041 ctccgtcagg tacgagcctt cgacgatcag gcagtactcg tccggctttt tatctttcat
     5101 gacgcgtgtg aagccctcgc cagtgtcggg ggaagcgcgc ccgccatcga ccgccgccgg
     5161 tcgcggctgc ggcgccagag gggcaaactt taccaccgtc gggttggcgc gtgccagaat
     5221 tttttcgacc cgcgggcgga cgaaaaaagc gccgccgggg gtcggtctcc ggcggcgctc
     5281 tcgtcgttca tccgcagtcg cggcggaggg cttcagtaag ccctgccgta ctgctcgttg
     5341 atggtcttgc tcaggcgctt gaagtacgcg tcgaaggtga cgtagtcggt gccctggaag
     5401 aaggcgcgac cgcccgtctc attggagagg cggttgagcg cgctctggcc gaaggagacc
     5461 gcgagccggc tgctgctcgt caacccgacc gacggcgcgt agaacgagta gacgaccacg
     5521 tcgcgcttct tcgcctcgct gatcgcgcgg tcgaggtcaa tcgagttgac ggacgacgtg
     5581 aagtcgaacc cgcgcgaggt gtcgaggccg tccgagacga ggagcagcgc gttgcggttc
     5641 gtgccgcccg ccgggaaacg cttcagcgcg tcgcgcactt ccacgtaagg gttgtagggc
     5701 gaacccgcgg tcgagcc
//
LOCUS       DBNGOH010000467         3479 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4285490, whole genome shotgun sequence.
ACCESSION   DBNGOH010000467 DBNGOH010000000
VERSION     DBNGOH010000467.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3479)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3479)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3479
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4285490"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            34..882
                     /locus_tag="AC159P_09240"
     CDS             34..882
                     /locus_tag="AC159P_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010041833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis family protein"
                     /protein_id="HIR3695677.1"
                     /translation="MTTTTETRTITVAHSPDSDDAFMFYGLATGKIDTGELRYEHVLE
                     DIQTLNEKAMQGVYDVTAVSFHAYAYVSDKYVLLPHGASIGEGYGPIVVAREPHAVAD
                     LPRLKIAVPGELTSAFLALRILCPEFEYEVVAFDRIIEAVREGRADAGLLIHEGQLFY
                     QNLGLHKVVDLGEWWKGWTDLPLPMGGNAIRRELGGDTIRRVSAYLHESIRYSLEHRQ
                     DALDYAMQFARDMDKGLADRFVGMWVNQLTLDYTERGRTAVQRLLDEGAARGVIPRRV
                     EAEFVS"
     gene            1134..2039
                     /locus_tag="AC159P_09245"
     CDS             1134..2039
                     /locus_tag="AC159P_09245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695678.1"
                     /translation="MKRFSLFALAAATLLTPLAVSAQTQQPASASTVGPPSVVNRSGA
                     PVDVDKIIRTFAAREAEFRKALNEYSFKRDAVLQTIAMGGQISGEYRRVSRFVFDDSG
                     NRFEKILFFPMPTLTAISVSPEDLEDLGGVQAFALENGKLHEYDFSYVGKEKIDDLDL
                     FVFDVSPKILKDERRLKELKKSKKPERFFEGRIWVDDRDFQIVKARGKGVPEFEQRFP
                     TFETYREQIDGKYWFPTYSFADDELIFKTGQSVRVRLRVKFEEFERLRGKVRIVEEGE
                     PGVLDKGIKEEPAAKPTPTPAKPKP"
     gene            complement(2139..2912)
                     /locus_tag="AC159P_09250"
     CDS             complement(2139..2912)
                     /locus_tag="AC159P_09250"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HIR3695679.1"
                     /translation="MRPAESSTTSAGRLGGRLWYWWSLFVAAMLLLVFGPPSIIVSYL
                     AGRREWVYPWALFGARNWLRLSGVKVSVRGREHLDPRQSYVFAANHRSFLDTATLFYY
                     TGRRIGIIAKKELLKVPIFGYGMGYVNIMAIDRSNRKRAIETMKAATDRLRRGVSFGV
                     FAEGTRALPGELLPFKKGAFYMAAEAGVPVVPVAIKNTDALMGKGTGVARPGEIEMVL
                     LPPVPTEGVKTDEEVRRLTEHVNALVAEELGVKVKGKGS"
     gene            2946..>3479
                     /locus_tag="AC159P_09255"
     CDS             2946..>3479
                     /locus_tag="AC159P_09255"
                     /inference="COORDINATES: protein motif:HMM:NF012471.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HIR3695680.1"
                     /translation="MRKSFRSHPFCLRSALCLFASAFLLCAAAAPAARSQQRQRPRPA
                     AGVETVRPAPPSPRSVLGFTPGDDRKVADWPEITNYFERLDRASDRVRVEQVGATTLG
                     RPLVAAFISAPENIRELAKYKDIQRRLADPRLVGSEAERDRLLREGKTVVAISCSIHS
                     TEIVGSQMSMQLAYELAA"
BASE COUNT          597 a         1209 c         1095 g          578 t
ORIGIN      
        1 tgtcgcggcg cgttcggatt cggcattgac gacatgacaa ccaccacgga aacacgcacc
       61 atcaccgtcg cgcactcgcc cgactcggac gacgccttca tgttctacgg gctggcgacg
      121 ggcaagattg acaccgggga gctgcgctac gagcacgtcc tcgaagacat ccagaccctc
      181 aacgagaagg ccatgcaggg cgtctacgac gtgacggccg tcagcttcca cgcctacgcg
      241 tacgtctcgg acaagtacgt gctgctcccg cacggggcca gcatcggcga ggggtacggc
      301 cccatcgtcg tcgcgcgcga gccgcacgcg gtcgcggatt tgccgcgcct gaagatcgcc
      361 gtccccggcg agttgacgag cgccttcctc gccctgcgca tcctctgccc cgagttcgag
      421 tacgaggtcg tcgcgttcga ccgcatcatc gaggcggtgc gcgagggccg cgccgacgcc
      481 gggctgctca tccacgaggg gcagctcttc taccagaacc tcggcctcca caaggtggtc
      541 gatctcggcg agtggtggaa ggggtggaca gacctgccgc tgccgatggg cggcaacgcc
      601 atccggcgcg aactcggcgg cgacacgatc cgccgcgtct ccgcgtacct gcacgagtcg
      661 atccgctact cgctcgaaca ccggcaggac gcgctcgact acgcgatgca attcgcgcgc
      721 gacatggaca aggggctggc cgaccgcttc gtcgggatgt gggtcaacca gttgacgctc
      781 gactacaccg agcgcgggcg caccgccgtc cagcgcctcc tggacgaggg cgccgcgcgc
      841 ggcgtcatcc cgcgccgggt ggaggccgaa ttcgtcagct agaacgattg cggaatgcgg
      901 atttcggatt gtggattgac ggaagcgcgc ctctcgtcaa cgacctgcgt gacgaaatcc
      961 cgcggctctc tcatcaatca cgcatccgca ctccgaaatc cgcattccgc aatcgattgg
     1021 tggcaacacg gccccgcccc aaagcatctg aaatggtgct catttcgcgg cgcgggttgt
     1081 tttcgacggc ctcgccgcta gtccaatcca agttttcgca tacgggaaaa gtcatgaaga
     1141 gattttcttt gttcgcgctc gcggcagcga cccttctgac gccgctcgcg gtctcggcgc
     1201 agacgcagca gccggccagc gcctcgaccg tcgggccgcc gtcggtcgtc aaccgttcgg
     1261 gcgcgccggt ggacgttgat aagatcatcc gcaccttcgc ggcgcgcgag gcggagttcc
     1321 gcaaggcgct caacgagtac agcttcaagc gcgacgccgt gctgcaaacc atcgccatgg
     1381 gcgggcagat ttcgggcgag taccgccgcg tctcgcgctt cgtcttcgac gacagcggca
     1441 accgcttcga gaagattctc ttcttcccga tgccgaccct gacggccatc agcgtctcgc
     1501 ccgaagacct cgaagacctc ggcggcgtcc aggcgttcgc gctcgaaaac ggcaagctcc
     1561 acgagtacga cttctcgtac gtcggcaagg agaagatcga cgacctcgac ctcttcgtct
     1621 tcgacgtgtc gccgaaaatc ctgaaggacg agcggcggct gaaggagttg aagaagtcga
     1681 agaagccgga gcgcttcttc gaggggcgca tctgggtgga cgaccgggac ttccagatcg
     1741 tcaaggcgcg cggcaagggc gtgcccgagt ttgaacagcg cttcccgacc tttgagacct
     1801 accgcgagca gattgacggc aagtactggt tcccgaccta ctcgttcgcg gacgacgagc
     1861 tgatcttcaa gaccggccag tcggtgcgcg tccgcctgcg cgtcaagttc gaggagttcg
     1921 agcggctgcg cggcaaggtg cgcatcgtcg aggagggcga gccgggcgtc ctcgacaagg
     1981 gcatcaagga ggagccggca gccaagccga cgccgacgcc tgcgaagccg aagccgtaac
     2041 ggtctccttc aaacgccgga aatagaaagg ccgggagcgt tggctcccgg ccttttccgt
     2101 ttcgcacttc atttcgcacg cgcgccgccg ccgcaccttc aactcccttt ccccttcacc
     2161 ttcacgccga gttcttccgc gacgagcgcg ttgacgtgtt cggtgagacg cctcacctcc
     2221 tcgtccgtct tcacgccctc ggtcgggacg ggcggcagca gcaccatctc gatctcgccg
     2281 gggcgcgcca cccccgtgcc cttgcccatc agcgcgtccg tgttcttgat ggcgacgggc
     2341 accacgggga cgcccgcctc cgccgccatg tagaaggcgc ccttcttgaa ggggagcagc
     2401 tcgcccggaa gcgcgcgcgt gccttcggcg aagacgccga acgagacgcc gcggcgcagg
     2461 cggtcggtcg cggccttcat cgtctcgatg gcccgcttcc ggttcgagcg gtcgatcgcc
     2521 atgatgttga cgtagcccat gccgtagccg aagatcggca ccttcagcag ctccttcttg
     2581 gcgatgatgc cgatgcggcg gcccgtgtag tagaagagcg tcgccgtgtc gaggaacgag
     2641 cggtggttgg cggcgaagac gtaagactgc cgcgggtcga ggtgttcgcg cccgcgcaca
     2701 ctgaccttga cgccgctcag ccgcagccag ttgcgcgcgc cgaagagcgc ccacgggtag
     2761 acccactcgc gccgcccggc gaggtacgag acgatgatcg agggcgggcc gaagacgagc
     2821 aacagcatcg ccgcgacgaa caaagaccac cagtaccaga gccgaccgcc gagccttccc
     2881 gccgacgttg tgctagactc tgccggtcgc actctcccgc tttcgtcgcc gccactttcg
     2941 atctcatgag aaagagtttc aggtcgcatc ccttttgcct tcgctccgcg ctctgcctgt
     3001 ttgcctctgc cttcctgctc tgcgccgccg ccgcgcccgc cgcccgctcg cagcagcggc
     3061 agcggccacg ccccgcggcg ggcgtcgaga cggtgcgtcc cgcgccgccg agtccgcgct
     3121 ccgtgctcgg cttcacgccc ggcgacgacc gcaaggtggc cgactggccc gagatcacga
     3181 actacttcga acgactcgac cgcgcgagcg accgcgtccg tgtcgagcag gtcggcgcga
     3241 ccactctggg ccgcccgctc gtcgccgctt tcatcagtgc gcctgagaat atacgggaac
     3301 tcgcgaaata caaagacatc cagcgccgcc tcgccgaccc gcgcctcgtc ggctcggagg
     3361 ccgagcgcga ccgcttgttg cgcgaaggca agacggtcgt cgccatctcc tgctcgatcc
     3421 actcgaccga aatcgtcggc tcgcagatgt cgatgcagct cgcctacgag ctggcggcg
//
LOCUS       DBNGOH010000468         1688 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4285510, whole genome shotgun sequence.
ACCESSION   DBNGOH010000468 DBNGOH010000000
VERSION     DBNGOH010000468.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1688)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1688)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1688
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4285510"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..419)
                     /locus_tag="AC159P_09260"
     CDS             complement(<1..419)
                     /locus_tag="AC159P_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018922129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3695681.1"
                     /translation="MEVTFANVTKKFGSTIVVDDLTLHVEDGEFVVLLGPSGCGKTTT
                     LRMLAGLEDATAGDILIGAERVNDVPARARDVAMVFQSYALYPHMTVAENIAYPLRVR
                     KMARDETGRRVERVAAMLEIESLLGRRPRELSGGERQR"
     gene            complement(634..1482)
                     /locus_tag="AC159P_09265"
     CDS             complement(634..1482)
                     /locus_tag="AC159P_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014762313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR3695682.1"
                     /translation="MRGGGRSGAGGRVAQYVLICLAVVAALVPVYWMLTVSLKTEAEQ
                     FASPPRWFAFAPTLAHYRDAFVARSFGQYLLNSALVAVVSTACALVLGTLAAYGLTRA
                     EVPRRRAERLSLWILSTRMFPPIVTVVPLFLMMRDLRLLNTLAALVVVHTAFNLPFVV
                     WMMRGFFREVPRELEEAALVDGDTRLGALRRVVLPLVAPGLAATAVFCLIVSWNEFLF
                     ALALAQTDAAMTLPVGIAGRVTQYEIKWGVMSAAGVVAMTPILVFALAVQRYLVRGLS
                     LGAVKG"
     gene            complement(1479..>1688)
                     /locus_tag="AC159P_09270"
     CDS             complement(1479..>1688)
                     /locus_tag="AC159P_09270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR3695683.1"
                     /translation="DLLRVFDQIFILTEGGPGFATETVSLYIYRTAFRFGDYGYAAAM
                     SFVLLVLTNTISVLYIRLLQRQEAT"
BASE COUNT          314 a          597 c          549 g          228 t
ORIGIN      
        1 cgctgccgct cgccgcccga cagctcgcgc ggcctgcgcc cgagcagact ttcgatctcc
       61 aacatcgccg ccacacgctc gacgcgccgc cccgtctcat cccgcgccat cttccgcacg
      121 cgcagcgggt acgcgatgtt ctccgccacc gtcatgtgcg ggtagagcgc gtaagactgg
      181 aagaccatcg ccacgtcgcg cgcccgcgcg ggcacgtcgt tgacgcgctc ggcgccgatc
      241 aggatgtcgc ccgcggtcgc gtcttcgagt cccgccagca tccgcagcgt cgtcgtcttg
      301 ccgcaccccg aaggcccgag cagcacgacg aactcgccgt cctcgacatg gagagtaagg
      361 tcgtccacga cgatggttga gccgaacttc ttggtgacgt ttgcgaacgt aacttccata
      421 cgggcaagga cgggattgcg gctccgccgc ctgatgtgcg gtgaggcttc acctcaccgg
      481 ggcgaagcct ctttctcggc tgaggctatg cctcagcacg ggggcgtagc cccggaattc
      541 aaaaagcgca ttcaacggaa gggcacagcc cttccgcaca tcaggcggca gagccgcaga
      601 agcagactta caaacaccgc gtcgagtttt gtctcacccc ttgaccgcgc cgagcgagag
      661 gccgcgcacg aggtagcgct ggacggcgag tgcgaagacg aggatcggcg tcatcgccac
      721 gacgcccgcg gcgctcatca cgccccactt gatctcgtac tgcgtgacgc ggcccgcgat
      781 gccgaccggg agcgtcatcg cggcgtccgt ctgcgcgagc gctagcgcga acaggaactc
      841 gttccacgag acgatgaggc agaagacggc ggtcgcggcg aggcccggcg cgacgagcgg
      901 cagcaccacg cgccggagcg cgccgagccg cgtgtcgccg tccaccagcg ccgcctcttc
      961 cagctcgcgc ggcacctcgc ggaagaaccc gcgcatcatc cacacgacga acggcaggtt
     1021 gaaggccgtg tgcaccacga cgagcgccgc cagcgtgttc agcagacgca gatcgcgcat
     1081 catcagaaac agcggcacga ccgtcacgat gggcgggaac atccgcgtcg agagtatcca
     1141 cagcgacagg cgctcggcgc ggcggcgcgg cacctccgcg cgcgtcaaac cgtaggccgc
     1201 cagcgtcccc agcacgagcg cgcacgcggt cgagacgacg gctacgagcg cgctgttcaa
     1261 gaggtactgc ccgaacgaac gcgcgacgaa ggcgtcgcgg taatgcgcga gcgtcggcgc
     1321 gaacgcgaac cagcgcggcg gcgacgcgaa ctgctcggcc tccgtcttca gcgagacggt
     1381 cagcatccag tagaccggca ccagcgccgc gacgaccgcg aggcagatca acacatactg
     1441 cgcgacccgg ccccctgcgc ccgaccgccc gccccctctc acgtcgcctc ctgtctttgc
     1501 agcaggcgga tgtagagcac gctgatcgtg ttcgtcagga cgaggagcac gaaggacatc
     1561 gccgcggcgt agccgtagtc gccgaagcgg aaggccgtgc gatagatgta gaggctgacg
     1621 gtctccgtgg cgaagccggg gccgccctct gtcagtatga aaatctggtc gaagacgcgg
     1681 agcaggtc
//
LOCUS       DBNGOH010000469         4061 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3736323, whole genome shotgun sequence.
ACCESSION   DBNGOH010000469 DBNGOH010000000
VERSION     DBNGOH010000469.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4061)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4061)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4061
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3736323"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            308..1195
                     /locus_tag="AC159P_09275"
     CDS             308..1195
                     /locus_tag="AC159P_09275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695684.1"
                     /translation="MGTCAFCGGRADGKRKLTLYSGHIEVSRSGETKTTTLSDIQRHE
                     LFVCDSCRQRRVVASLLAVAACCLWLGWFGWATTRREFPLFEFLIVTGTVVGAFAAIL
                     VRRFRQKAYLTRRIRDERGDQSFDIVDGADQLSLGNVLEAMSATQRSATVRRKDGYSY
                     GAGQSDLRYELENYSAANGFPVAHVADAVCRCGGRTFSLALDAEARVALRVCGKCGDE
                     HSIGDSAERGAADAAASCECVCGKTQFEITLGVALDEGAEDALWLYIACRCPGCELMA
                     VYADWKTESIGYKELLARA"
     gene            1297..2091
                     /locus_tag="AC159P_09280"
     CDS             1297..2091
                     /locus_tag="AC159P_09280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695685.1"
                     /translation="MAVVLLAVGLVLAGLAYSFYSEHTRAGNVARFYRTHADEMPTSG
                     SREERIAGELRSADRNRNLAMLSGAGGLLLCAGGAALFMLSRRVEAVEVSPPGGTYED
                     PAAQSEAIDRWASVALTRPVTVQYRRLHTVLLASVLAFFTGMSLLMVVANGLTGVTVL
                     LLILNGLLLVALSYLARRARRRAASRFDLSGVTRGDQRLLSWGEFKGVDYLMAIKPRS
                     GREYLWRVELRFVGGVAWIIPRRVVNLPEVIELVGTLPGTHRKRRA"
     gene            2248..2817
                     /locus_tag="AC159P_09285"
     CDS             2248..2817
                     /locus_tag="AC159P_09285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695686.1"
                     /translation="MNANVSTLCFAARTYGAVLLALCCAGCQLFGDPAQENRDRLYAR
                     LEEFSQIPPREQLTDQPYIRGRALVVTKKQDPPITMEDPITWGSDKAKELMAEAPEQV
                     ETVVVLNYSKDKVATYNIVEGKGGVYGYRESCEVILIDRSIPAVIHRKTFRGPELPPT
                     KIIRETQGEVLSSVDLTTVRNYVMNIPRR"
     gene            3088..>4061
                     /locus_tag="AC159P_09290"
     CDS             3088..>4061
                     /locus_tag="AC159P_09290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695687.1"
                     /translation="MNTRPFLLLLFSLFVFANKAFAQAPPAAKSAQAAAPVVTVSATP
                     SGVRFAALGSVKQTRLEVYDSAGAPVLDTGFLAGNVRDWQPQAGGLADGTYQCVLSAR
                     DLAGRLSLKQAAVLVAGGAASLSLGEAEASGGVSSVAQAGEPGAATLTTHDGKDGAVT
                     ATSGDLTLRTGDVLAGEDEERVRVTAEGKVGVGTSKPEAALDVAGEVRASQGFRFSDG
                     SRLAVDEQGTLVRTDAAGAPMPALSAAGTGTLNRVAKWLETGGAGTLIDSALVESGGN
                     VGLGTTTPVGSLSIGGSTKPTPMTMNYQYFSPNSIVDQGLIGWNYYWNG"
BASE COUNT          677 a         1319 c         1347 g          718 t
ORIGIN      
        1 ggcgctcatc atcgacgcgc cggctactta tgtttagtca ctcccatcga cccgactcta
       61 ttgacccgcc gccggcagag gcgtatcttc tcaccgctcc gaacgcgtac cggtctgaac
      121 tctaggcttg cgctcccgac cgcacccctc ctgcttcagt cacttgcttc gtcccgcggc
      181 tcaacgctcc gagaaaggag actttcgagg tgccacctcc tgctaaacca ctaccggcgg
      241 cggacggaat ccaagggcgg ggacgggcag tttggcgcgg ggcttcgagc acggtctgag
      301 ggaggtcatg ggcacctgtg cgttctgcgg cggacgggcg gatggtaagc gaaaacttac
      361 gctctactcc ggacatatcg aagtgtccag gtcgggtgag accaagacca cgactcttag
      421 tgacatccag cgccacgagc tgtttgtttg tgactcttgt cggcaacgtc gcgttgtcgc
      481 ctccttactg gcggtggcgg catgttgtct ctggctgggc tggttcggct gggctacgac
      541 tcgacgagag ttccccctgt tcgagtttct gatcgtgacc gggacggtcg taggcgcgtt
      601 cgcggcgata cttgtccgac gttttcggca gaaggcttac ttaacccggc gcattcgaga
      661 cgagcgcggc gaccaatcct tcgacatcgt ggacggcgcc gaccagcttt cactgggaaa
      721 cgtgctggaa gcgatgtcgg ccacgcaacg tagcgccacg gtaagaagga aagacggcta
      781 ctcctatggc gccgggcagt cggatctgcg ctacgagctt gaaaactaca gcgccgcgaa
      841 cggctttcct gtcgcccatg tggccgacgc tgtatgccgt tgcggcggac ggacgttttc
      901 gctggcactc gatgccgagg cgcgcgtcgc cctgcgcgtc tgcggcaagt gcggagacga
      961 gcattcgatc ggcgacagcg ccgagcgcgg ggcagccgac gccgcggcat cgtgtgaatg
     1021 tgtctgcggt aagacgcaat tcgagatcac gctgggcgtg gcgcttgacg aaggggccga
     1081 ggatgcgctc tggctgtata tcgcgtgtcg gtgtccgggt tgtgagctga tggccgtcta
     1141 tgcggactgg aagaccgagt ccatcggata taaggaactc ctggcccgtg cgtagtcgga
     1201 actttcgcgg agcgttgcaa agcgtccctt ccagcttccg cctcggcagg ccgcgagcgg
     1261 ctgtgatcgg cagaaaggga tgctatgcgg aaggtcatgg cggtcgttct cctggcggtc
     1321 ggcctcgtcc tcgccgggct cgcctactcg ttttattccg agcatacgag ggccggcaac
     1381 gtggcgcgtt tttaccggac gcacgccgac gaaatgccga cgagcggcag ccgcgaagag
     1441 cgcatcgccg gggaactgag gtctgccgac agaaatcgca atctggcgat gctgagcggc
     1501 gcgggcggcc tcctgctctg cgccggcgga gcagcgctgt tcatgctcag ccggcgtgtc
     1561 gaagcggtcg aggtctcgcc gccaggcggc acgtacgaag acccggcggc gcagtcggag
     1621 gcgatcgacc gctgggcgag tgtcgcactc acgcgccccg ttacggttca gtaccgccgg
     1681 ctccataccg tcctgcttgc ctccgtcctg gccttcttca ccggcatgag cttgctcatg
     1741 gttgtcgcca acggcctcac cggcgtaacc gttctgctgc tcatcctgaa cggcctcctg
     1801 ctcgtggcgc tgtcttacct ggcaaggcgc gcgcggcgca gagcagcgtc ccgcttcgac
     1861 ctgtcgggtg tgacgcgcgg cgaccagcgg cttctgagtt ggggtgagtt caaaggcgtg
     1921 gattacctga tggccatcaa accgcgcagc ggcagagagt acctgtggcg cgtcgagctt
     1981 aggttcgtag ggggcgtagc gtggatcatt ccgcggcggg tcgtgaactt gcccgaggtc
     2041 atcgagctgg tcggaacgct ccccgggacg caccggaaac gcagggcgta ggcgggaccc
     2101 gctcttattt tcctgcacag gctcgggccg gcgggcgacg ccgggcgtca ggttctcatc
     2161 accaacagtg cgggggagcc gggcgttccc cgcacagtgt cagggacgaa acatcttcgg
     2221 cacgcgtgtg cgataaggag gattagcatg aatgccaacg tctcgaccct ttgcttcgcg
     2281 gcgcgcacgt acggcgcggt gttgctcgcc ctctgctgtg ccgggtgcca gctcttcggc
     2341 gacccggcgc aagagaatcg tgaccggttg tacgcgcgtc tcgaagaatt ttcccaaata
     2401 ccgccgcgcg aacaactcac ggatcagcct tacatcagag gccgggcgct ggtcgtcacg
     2461 aaaaaacagg acccgcccat cacgatggaa gacccgatca cctggggctc tgacaaagcc
     2521 aaagagctga tggcggaagc gcctgagcag gtcgaaacgg tcgtggtgtt gaattactcc
     2581 aaggataaag tggccaccta taacatagtg gaggggaagg gtggcgtcta cggctacagg
     2641 gagagctgcg aggtgattct catcgaccgc tcgatccccg ccgtcatcca ccggaagact
     2701 tttcgcgggc cggaactgcc gccgacgaag atcatccgcg agacacaagg cgaagtgttg
     2761 tcgtcggttg acctgaccac ggtgcggaac tacgtcatga acatcccccg caggtagcga
     2821 cctcctgacg gcgtgacaaa ctccatagag tcggcgcaac ttcttgcagc cgcccggcag
     2881 atgccccaga aggcggcgtg cgccctcgcg gtcggtcacc gagccggccg tgaccagcgc
     2941 cgcccgcaaa accttgactc cgctggccca cgcgcgccat gatgcgccgt ccctatcttt
     3001 cccgggccgc gccttctggc gccgcccact ccctacgacc cgtacgcgct cttgccgcca
     3061 tgctcctaca tgcctaagga gtcccagatg aacacgcgcc ccttcctcct cctgctcttc
     3121 tcgctcttcg tcttcgccaa caaggctttc gcgcaggcgc caccggcggc gaagtcggcg
     3181 caggccgccg cgcccgtggt gacggtctcg gccaccccgt caggcgtgcg gttcgccgcg
     3241 ctcggcagcg tcaagcagac ccgccttgag gtctacgatt ctgcgggcgc gcccgtcctc
     3301 gacacgggct tcctcgcggg caacgtccgc gactggcaac cgcaagcggg cggactcgcc
     3361 gacgggacgt atcagtgcgt gctgagcgcg cgcgacctcg cggggcgcct cagcttaaag
     3421 caggcggcgg tgctcgtcgc cgggggcgcc gcttctctgt cgctcggcga ggccgaggcg
     3481 tcgggcgggg tatcttccgt ggcacaggcc ggggagccgg gcgccgcgac cctcacgacg
     3541 cacgacggga aggacggcgc ggtgacggcc acctccggcg atttgacctt acgcaccggc
     3601 gatgtactcg cgggcgagga cgaggagcgc gtgcgcgtca ccgcggaggg caaggtcggc
     3661 gtcggcacct cgaagccgga ggcggcgctc gacgtggccg gggaggtcag ggccagccag
     3721 ggcttccgct tctctgatgg gagccggctc gccgtcgatg agcagggcac tctcgtgcgc
     3781 accgacgcgg cgggcgcccc catgccggcc ctctccgccg ccggcacggg caccctcaac
     3841 cgtgtggcca agtggctgga gacgggcggc gcgggcacgc tcatcgactc ggccctcgtc
     3901 gagtcgggcg gcaacgtcgg tctggggacg acgacccccg tcggcagcct gagcatcggc
     3961 ggctcgacca agccgacgcc gatgacgatg aactaccagt acttctcgcc caactccatc
     4021 gtcgatcagg gcctcatcgg ctggaactac tactggaacg g
//
LOCUS       DBNGOH010000470         7992 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1720787, whole genome shotgun sequence.
ACCESSION   DBNGOH010000470 DBNGOH010000000
VERSION     DBNGOH010000470.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7992)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7992)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7992
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1720787"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="AC159P_09295"
     CDS             <1..199
                     /locus_tag="AC159P_09295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695688.1"
                     /translation="VRNEADQTLTRNEEFVKSQDPDEIQRALQEVELLAGQLTMAMLN
                     PSGGDQRGANKIEFDDPNERL"
     gene            291..1427
                     /gene="dnaJ"
                     /locus_tag="AC159P_09300"
     CDS             291..1427
                     /gene="dnaJ"
                     /locus_tag="AC159P_09300"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019098160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HIR3695689.1"
                     /translation="MSKRDYYEVLGVARGASDAEIKSAYRKLAVRYHPDKNPGDQEAE
                     EKFKEAAEAYSVLSVAEQRARYDRFGHAGVSGAGAGAAGWGAQGFGGIEDILGDLFGF
                     SDMFGGRGGGGGGGGRRTAAQRGADLRYDLEMTLEEAAAGMTAQLRIPRLEGCEKCAG
                     TGAAPGTQPEQCATCGGSGQVRYQQGFFSVARTCSNCRGTGRVIRTPCDECRGAGRVE
                     REKQMEVKIPAGVETGSRLRLAGEGEAGTQGGPAGDLYVVIHVKEHELFERQGNNLYA
                     AVPVTFAQAALGSEVSVSTLEGQQMLKVPAGTQTGTVFRLKGNGMPALGGRGRGDLFV
                     SVTVITPTTLTREQRRLLEQLAEIEEKDIDEKGGLMDKVRDIFG"
     gene            1740..2054
                     /locus_tag="AC159P_09305"
     CDS             1740..2054
                     /locus_tag="AC159P_09305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695690.1"
                     /translation="MFAMQATALASDDGDDTDTRLVDAKVVEVTEHRISVIARTGVEH
                     VIAIDGSDTKVWLKGKAVSLKDLQKGDVVTVELDELNPVKFAKQIRIAVQSDSELATA
                     VP"
     gene            complement(2278..3354)
                     /gene="tsaD"
                     /locus_tag="AC159P_09310"
     CDS             complement(2278..3354)
                     /gene="tsaD"
                     /locus_tag="AC159P_09310"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HIR3695691.1"
                     /translation="MLVLGIESSCDETAAAIVRDGREMLSSVISSQVDIHKLYGGVVP
                     ELASREHLDKIVPVVEEAFARAGVVPEEVDGVAVTVGPGLVGSLLVGVSYAKAMAYAL
                     GKPFVGVNHIEGHVYSVVFENPPIEYPAFALVVSGGHTNLFHVPEPGRYKVLARTRDD
                     AAGEAYDKVAKMLGLGYPGGPIIERLALEGDPRAVRFSIPRMGDGRPDFSFSGLKTAV
                     SKHVRETGLKPVAAGEEASQAVKDLAASFQDVVIRSLVGTTERLAEEHRPHTLIVAGG
                     VACNNALRAASHEAGRRLGIPVYFPSRHLSTDNAAMIAAAGTVKLQAGQRDGLDLNAD
                     VSLRLQNLEVEDEALRQSKVRYKL"
     gene            complement(3623..4810)
                     /locus_tag="AC159P_09315"
     CDS             complement(3623..4810)
                     /locus_tag="AC159P_09315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695692.1"
                     /translation="MARMIHALKCPACSAPVDYEDQSGSRTVRCPFCNNTVVVPGAAG
                     AGGGWHPQVSATTVSVGRGEASAVVRRALVVIPIVVVAGIVVTAVVAYKFVSETTGQV
                     SRTIEVRRNDVTKTEPPRAKVEPPKPPAFATQIHKFGGEGTGPGLFKDARSIAVDGEG
                     RIYVGDYTGGRVQVFDAAGNFQTQWMVDAKMPLLDLDADRKGTVYVVQSGKILRYEGA
                     TGKPLGEVGHERGWFDDVNVTADGGLVAAWRRQTDDIVRFDASGRVLKTVRAAISGQT
                     DRSELNMKVAVDGLGNVYALGTFNEAVLKFTPEGKFVTKFGSSGDQPGQFRAAQSIAV
                     DGQGRVYVSDFRGVQVFDAEGRYLDVFKPEGIAFGMTFNDRNELFVAARTQVLKLSIN
                     KKP"
     gene            complement(4861..5262)
                     /locus_tag="AC159P_09320"
     CDS             complement(4861..5262)
                     /locus_tag="AC159P_09320"
                     /inference="COORDINATES: protein motif:HMM:NF024092.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR3695693.1"
                     /translation="MSQENVNVIRGMYDAFAAGDIPVVIAALDPLIEWWEAENFIYAD
                     RNPYVGPSAVLEGVFARLGNEWEGFAVSPEGVLDAGDTVVGHGYYSGTYKANGGRVRA
                     QFAHFFTFRDGKVVKFQQYTDTAQFARATGG"
     gene            complement(5361..5813)
                     /locus_tag="AC159P_09325"
     CDS             complement(5361..5813)
                     /locus_tag="AC159P_09325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695694.1"
                     /translation="MTSADILSRLRRFLLVFCVLLFGGAVVELWLVNHKEDKLQLIPF
                     ALCALGTVAALVALFRPRRATLWLLRGTMALVVCGTLLGIYLHLDGNIDFQREIDPDA
                     PASDMLLSVLGGANPLLAPGILSVAAVLALAATYYHPALVGGEGEESD"
     gene            complement(5810..6376)
                     /locus_tag="AC159P_09330"
     CDS             complement(5810..6376)
                     /locus_tag="AC159P_09330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695695.1"
                     /translation="MPRATVFRAFMSLLVLSSSLLVAACGSGAEKAPANTPATNTNGA
                     ANTSGTPAAAATAAAAPGAPKAKLNVNTASRDEFLAAVPNLGNKMAHEFEEYRPYRSI
                     QQFRKEIGKYVDAAKVAEYEQYIFVPIDENGSDAATLQQIPGLDATEAAALVAARPYA
                     SREAFLAKLSEKVSADELAVARTYMGAK"
     gene            complement(6603..7406)
                     /locus_tag="AC159P_09335"
     CDS             complement(6603..7406)
                     /locus_tag="AC159P_09335"
                     /inference="COORDINATES: protein motif:HMM:NF024819.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside adenylyltransferase
                     domain-containing protein"
                     /protein_id="HIR3695696.1"
                     /translation="MSALISQLEPHPEVDALLREVLEEVRAVLGERFVGAYLFGSLTS
                     GDFDEASDIDLLFVTDEEMPPESFAALQSMHARINARDTHWATQLEVSYIARRALRRR
                     DPYDCLHPHLDRGRGETLRPREHDSDWVVQRHVLRERGLTLAGPDPRTLIEHVSPDDL
                     RRAMHDLLWWPAEILGDPARIDTRGYQSYLVLTMCRVLYTLRHGAVVSKRAAADWAQE
                     TLDARWSPLIERARAGRHEPRLAATTEDVNGTLDLIRHTLEQARRDETT"
BASE COUNT         1400 a         2520 c         2705 g         1367 t
ORIGIN      
        1 cgtgcgcaat gaggccgacc agacgctcac gcgcaacgaa gagttcgtca agtctcaaga
       61 ccccgacgaa atccagcgcg cgctgcaaga ggtcgagctg ctggccgggc aactcacgat
      121 ggcgatgctc aacccgtccg gcggcgacca gcgcggcgcc aacaagatag aattcgacga
      181 ccctaacgaa aggctttgaa agccgtcaac cgtaaaccgt aaaccgtgac aagtcggaat
      241 cggttctctc ttgtcgcggt ttacggttta cggttcacgg catttaggac atgagtaaaa
      301 gagactacta cgaagtgctc ggcgtggcgc gcggcgcgtc cgacgcggag atcaagagcg
      361 cctaccggaa gctcgccgtg cgttaccacc cggacaagaa tccgggcgac caggaggccg
      421 aggagaagtt caaggaggcg gcggaggcgt actccgtcct ctccgtggcg gagcagcgcg
      481 cgcgctacga ccggttcggc cacgcgggcg tgtcgggcgc gggcgcgggc gccgcgggct
      541 ggggcgcgca gggcttcggc ggcatcgaag acatcctcgg cgacctcttc ggcttcagcg
      601 acatgttcgg cgggcgcggg ggcgggggcg ggggcggcgg tcggcgcacg gcggcgcagc
      661 gcggcgcaga cctgcgctac gaccttgaga tgacgctcga agaggccgcg gcggggatga
      721 ccgcgcagct ccgcatcccg cggctcgaag gctgcgagaa gtgcgcgggg acgggcgcgg
      781 cgccggggac acagcccgag cagtgcgcga cgtgcggcgg ctccgggcag gtgcgctacc
      841 agcagggctt cttctcggtg gcgcgcacgt gctcgaactg tcggggcacg ggccgcgtca
      901 tccgcacgcc gtgcgacgag tgcaggggcg cgggccgcgt cgagcgcgag aagcagatgg
      961 aggtgaagat accggcgggc gtcgagaccg gctcgcggct gcggctcgcg ggcgagggcg
     1021 aggccggcac gcagggcggc cccgctggag acctctacgt cgtcatccac gtcaaggagc
     1081 acgagctgtt cgagcggcag ggcaacaacc tgtacgccgc cgtgcccgtc accttcgcgc
     1141 aggccgcgct cggctcggag gtcagcgtct ccacgctcga agggcagcag atgttgaaag
     1201 tccccgcggg cacgcagacc ggcaccgtct tccgcctcaa gggcaacggc atgcccgcgc
     1261 tcggggggcg cggtcgcggc gacctcttcg tctccgtcac cgtcatcacg ccgaccacgc
     1321 tcacgcgcga acagcgccgc ctcctcgaac aactcgccga gatcgaagag aaagacatcg
     1381 acgaaaaggg cggcctgatg gacaaggtgc gcgacatctt cggataaggc aaaggggaag
     1441 cgtgtaaaag gggaagcggc gaaaagggag gaaagcaaac cgcttcttac cttttcgcct
     1501 cttccccttt tacactcttc cccctccttc gtttgtatcg atacaaaacg tcctctcacg
     1561 gtacaaacaa cacccggcga acaccctccg acggaagttt ttcccgcggt tccttttaaa
     1621 atcaggcatt aagcggggca ccgcgccgcg gcatgttgct tgccatggag actggcatgt
     1681 gcgaaggggg gattaagatg ggacggcggc acctcgcagt aatagcggcg gcgctcttga
     1741 tgtttgccat gcaggcgacg gccctcgcgt ccgacgacgg cgacgacacg gacacgcggc
     1801 tggttgacgc gaaagtggtg gaggtgacgg aacaccgcat ctccgtcatc gcgcgcaccg
     1861 gcgtcgagca cgtgatcgcg attgacggct cggacacgaa ggtctggttg aagggcaagg
     1921 ccgtgtcgtt gaaagacctg caaaagggcg acgtggtgac ggtcgagctt gacgagctga
     1981 accccgtcaa gttcgccaaa cagatcagaa tcgcggtgca gtccgacagt gaactggcga
     2041 ccgccgtccc ataacacttt cggctgaacc gcgaacactt agccggtcat tgcacgggcc
     2101 ttgtttcaag cgtctctgct tcgccggcaa atagaaagcc gctgcgcctc cttcgcaata
     2161 aggcgcagcg gctttcgaat attgcggctt gcggattaca gaaagaagaa ggttgacggc
     2221 agcggcgctc tcgtcaacct tctttgcttt ttcttccaat ccgcattccg caatcgttca
     2281 gagtttgtac cgcaccttcg actgccgcag cgcctcgtct tccacttcca ggttctgcaa
     2341 acggagcgac acgtctgcgt tgaggtcgag gccgtcgcgc tggccggcct ggagtttgac
     2401 ggtgcctgcg gcggcgatca tcgcggcgtt gtcggtcgag aggtggcgcg aggggaagta
     2461 gacggggatg ccgagccggc ggcccgcctc gtgcgaggcc gcgcgcagcg cgttgttgca
     2521 ggccacgccg cccgcgacga tgagcgtgtg cgggcggtgc tcttcggcga gccgttcggt
     2581 cgtgccgacg agcgagcgga tgaccacgtc ctgaaaactc gccgccaaat ccttcaccgc
     2641 ctgcgaagcc tcttcgcccg ccgcgaccgg cttgagtccg gtctcgcgga cgtgcttcga
     2701 gacggcggtc ttcaagccgc tgaagctgaa gtcgggccgc ccgtcgccca tgcgcgggat
     2761 gctgaagcgc acggcgcgcg ggtcgccttc gagcgcgagc cgctcgatga tcggccctcc
     2821 gggatagccg aggccgagca tcttcgccac cttgtcgtaa gcctcgcccg ccgcgtcgtc
     2881 gcgcgtgcgc gcgagcacct tgtagcggcc cggctccggc acgtggaaga ggttcgtgtg
     2941 cccgcccgag acgacgagcg cgaaggcagg gtattcaatc ggcgggttct cgaagaccac
     3001 ggagtagacg tgcccctcga tgtggttgac gccgacgaac ggtttgccga gcgcgtaggc
     3061 catcgccttc gcataggaga cgccgaccag cagcgagccg acaaggccgg ggccgaccgt
     3121 gacggcgacg ccgtcaacct cttcgggcac gacccccgcg cgcgcgaacg cttcttcgac
     3181 gacgggcacg atcttgtcga ggtgctcgcg cgaggcgagt tccggcacga cgccgccgta
     3241 gagcttgtgg atgtccacct gcgaggagat gacggacgag agcatttcgc gcccgtcgcg
     3301 gacgatggct gccgccgtct cgtcgcagga gctttcgata ccgagtacga gcatgatgtt
     3361 gagatgataa caggccgggc cggtcggtta ataatgcgcg tgagtcgaga ggcggcaggg
     3421 gaggtaatac cgaccggcga ggatatctct tggcggctgt gccgcctgtt gtgcggtgag
     3481 gctgagcctc accggcagcg cctcttcttg tttagctgag gctgcgcctc agcgcagggg
     3541 cgtagcccaa aaattcaaac gcacactctc ggaagggcac agcccttccc acatcgggcg
     3601 gcggagccgc ttaagcaata cgtcacggct tcttgttgat cgagagcttc aagacctgcg
     3661 tgcgcgcggc gacgaacagc tcgttccgat cgttgaaggt catgccgaaa gcgatgccct
     3721 cgggcttgaa cacgtcgagg tagcggcctt cggcgtcgaa gacctgcacg ccgcggaagt
     3781 cgctgacgta gacgcgcccc tgcccgtcca ccgcgatgga ctgcgcggcg cggaactggc
     3841 cgggctggtc gccgctgctg ccgaacttcg tcacgaactt tccttcgggc gtgaacttca
     3901 agacggcctc gttgaaggtg ccgagcgcgt agacgttgcc gagtccgtcc acggcgactt
     3961 tcatgttcag ctcgctgcgg tcggtctgtc cgctgatggc ggcgcgcacg gtcttgagga
     4021 cgcggcccga cgcatcgaag cggacgatgt cgtcggtctg ccggcgccaa gcggcgacga
     4081 ggccgccgtc cgccgtcacg ttcacgtcgt cgaaccaccc gcgctcgtga ccgacctcgc
     4141 ccagaggttt gccggtcgcg ccctcatagc gcaggatttt gccggactgc acgacgtaga
     4201 cggtgccctt gcggtctgcg tccaggtcga gcagcggcat cttcgcgtcc accatccact
     4261 gcgtctggaa attgcccgcc gcgtcgaaga cctgcacgcg gccccccgtg tagtcgccga
     4321 cgtagatgcg cccctcgccg tccacggcga tgctgcgcgc gtccttgaac agccccggcc
     4381 cagtgccctc gccgccgaac ttgtgtatct gagtggcgaa ggccggcggc ttcggcggct
     4441 ccactttcgc gcgcggcggc tccgtcttcg tgacatcgtt ccgcctgacc tctatcgtcc
     4501 gcgagacctg accggtcgtc tcgctgacga acttgtaggc gacgacagcg gtgacgacga
     4561 tgcccgcgac gacgacgatg gggatgacca ccagcgcgcg ccgcacgacc gcggaggcct
     4621 cgccgcggcc cacgctgacc gtggtcgcgg aaacctgcgg gtgccagccg ccgcccgctc
     4681 ccgccgcgcc gggcacgacc accgtgttgt tgcaaaacgg gcagcggacg gtacggctgc
     4741 cgctctggtc ttcgtagtcc accggcgccg agcaggccgg acacttgagc gcgtgaatca
     4801 tgcgggccat tctaccaacg accgcggcga tagccgaggg cgagcgcgag gcgggacgtg
     4861 ctatccgccg gtggcccgcg cgaactgcgc cgtgtccgtg tactgctgga acttcaccac
     4921 cttcccgtcg cggaaggtga agaagtgtgc gaactgcgcg cggacgcgcc cgccgttcgc
     4981 tttgtaagtg ccgctgtaat agccgtgccc gacgaccgtg tcgcctgcgt ccagaacccc
     5041 ctcgggcgag acggcgaacc cttcccactc attcccgagc cgcgcgaaga cgccttccag
     5101 cacggcgctc gggccgacgt aaggattcct gtcggcatag ataaagttct cggcctccca
     5161 ccactcgatc agcgggtcga gcgcggcgat gacgacgggg atgtcgcccg ccgcgaatgc
     5221 atcgtacata ccgcggatga cgttgacgtt ctcctgtgac attggggcct ccttcgggtt
     5281 ttgggttatc ggcgcgaggg gaactccggc gaatcacgaa cgggaatact tttaccgctt
     5341 cgacgcttac cacgcactgc ttagtcgctc tcctcgccct cgccgccgac gagggccggg
     5401 tggtagtagg tggcggcgag cgcgaggact gcggcgacgg agaggatgcc gggcgcgagg
     5461 agcgggttgg cgccgccgag gacgctgagc agcatgtcgg aggcgggcgc gtcgggatct
     5521 atctcgcgtt ggaagtcgat gttgccgtcg aggtgcaggt agatgccgag gagcgtgccg
     5581 cagacgacga gcgccatcgt gccgcgcagc agccagaggg tcgcacgccg cgggcggaag
     5641 agcgcgacga gcgcggcgac cgtgccgagc gcgcacagcg cgaacgggat cagttgcagc
     5701 ttgtcttcct tgtggttgac gagccacagc tcgaccaccg cgccgccgaa cagcaggaca
     5761 cagaagacga gcaggaaccg ccggaggcgc gagaggatgt cggcggaagt cacttggcgc
     5821 ccatgtaggt tcgcgcgacg gccagctcgt cggcggagac cttctcggag agtttggcga
     5881 ggaaggcttc gcgcgaggcg taggggcggg ccgcgacgag cgccgcggcc tccgtcgcgt
     5941 cgaggccggg aatctgttgc agcgtcgccg cgtcggagcc gttctcgtca atcggcacga
     6001 agatgtactg ctcgtactcc gcgaccttcg ccgcgtcaac gtacttgccg atctctttgc
     6061 ggaactgctg gatgctgcgg tagggccggt actcctcgaa ctcgtgcgcc atcttgttgc
     6121 cgaggttggg gacggcggcg aggaattcat cccttgaggc cgtgttgacg ttgagcttcg
     6181 ccttcggggc gccgggcgcg gcggctgcgg tggccgcggc ggcgggggtg ccggacgtgt
     6241 tggcagcgcc gttcgtgttg gtcgcggggg tgttcgcggg cgccttctcc gcgcctgaac
     6301 cgcaggcggc gacgagtaga gacgaagaca gaacgagcag ggacatgaag gctctgaaga
     6361 ctgtggcacg cggcatcatt gatcttcctt tcgaacacat caccgggatt gaacaggcga
     6421 cgccattata cttggcgtcg cctgctttac taatccgcca gggcggctgc tggcgggtga
     6481 gtttagcgca ggttaattcg aacacagagg gcacggagga gacacgaaga gcacagagca
     6541 gaaacggttt tctccgtgcc ctctgtgttg agatgaacgc ccggtaagct cgacgacgat
     6601 gatcaagtcg tttcgtcgcg acgtgcttgt tcgagcgtgt ggcggatgag gtcgagcgtg
     6661 ccgttgacat cttcggtcgt cgccgcgagg cgcggctcgt gtcgcccggc ccgtgcgcgt
     6721 tcgatgaggg gcgaccagcg cgcgtccaga gtctcctgcg cccagtcggc ggcggcccgt
     6781 ttggagacga cggcgccgtg ccggagcgtg tagaggacgc ggcacatcgt gaggacgagg
     6841 taggactggt agccgcgcgt gtcgatgcgg gccgggtcgc cgaggatttc ggcgggccac
     6901 cagagcaggt cgtgcatcgc gcggcgcagg tcgtcgggcg agacgtgttc gatgagggtg
     6961 cgcgggtcgg ggccggccaa tgtcagaccg cgctcgcgca ggacgtggcg ctgcacgacc
     7021 caatcgctgt cgtgctccct cggtcgaagc gtctcgcccc ggcccctgtc gaggtgcggg
     7081 tgcaggcagt cgtaagggtc tctgcggcgc agcgcgcggc gcgcgatgta ggagacttcg
     7141 agctgcgtgg cccagtgcgt atcgcgcgcg ttgatgcgcg cgtgcatcga ctgcaaggcc
     7201 gcgaacgact cgggcggcat ctcttcgtcg gtgacgaaga ggaggtcgat gtcgctggct
     7261 tcgtcgaagt cgccgctcgt cagcgagccg aacaggtacg cgccgacgaa gcgttcgccg
     7321 agcacggccc gcacctcttc cagcacctcc cgcagcagcg cgtcaacctc cgggtgcggc
     7381 tcaagctgac tgatcaaggc ggacattctt cactacgcct cttcgatccg cctctatccg
     7441 cgatcaatcc gaaagataag aagaacttct ctttatccct cgaaacatct ctattcgcag
     7501 gagtaacaat aactattggc atgtacacgc ggttgccgac cacgtacagc ctcgtcgtat
     7561 ctccggctac ccctccgggg gcgacgatat tcaactcccg gcccggataa ccgttaatgc
     7621 cgagcttgga ttcattcagt aaccgactat tcgtactccg aatcaaagca tcccgtgccc
     7681 cgtccaacgc ttgttcgggg ttaggaagag cactttggta atcagtatac ccggcggcgt
     7741 atgtggccca gtcagtatcg gcgatgaaag aacgggtgac acctagctat cgagcggtcg
     7801 gcttgtgcga cttgttaggc cgaactgtcc atggcattaa caacctccat tattccatgt
     7861 gccacatcca aacatacttg gtctcgattt ccccattgag tgatcgcact ttgcgccacc
     7921 tgcgcccggt tgggaacatg cccgacgccc gaatttgtca cccgcgccga gcagatagca
     7981 ttcaagccga ag
//
LOCUS       DBNGOH010000471         6924 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_70761, whole genome shotgun sequence.
ACCESSION   DBNGOH010000471 DBNGOH010000000
VERSION     DBNGOH010000471.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6924)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6924)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6924
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_70761"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            296..2755
                     /locus_tag="AC159P_09340"
     CDS             296..2755
                     /locus_tag="AC159P_09340"
                     /inference="COORDINATES: protein motif:HMM:NF012371.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HIR3695697.1"
                     /translation="MSVWRRCLNVALAAALAAAWAPSLTLQRVSAQAAAPAPPQSQTE
                     LEAKLAAAEKAIEEKRRELGVPGAALVVVKDDRVVYMKGLGVKDFEKRQPVTPDTLFA
                     IGSSSKAFTAMLVAMAADAGKLSFDDPPRKFLPYFKLQDAETDARITVRDLLSHSSGL
                     NRTDIAWITGTLTREEVIRVAAQAKPTAKLREKFLYQNVMYTAAGEVAARAEGSTWER
                     LIRERIFGPLGMKASVLTAPEMQRAPDHSFGYEYDEATKETRRLPQRDFPQVAAAGAI
                     NSNARDMAGWLRLMLGGGAFEGKRLVSEKSFAELVKPQQKIAGSISYGLGWFLRDWHG
                     HKVVEHGGNIDGFNAQVALMPDQRLGFVLLTNVTASALPATAMEAVWSNLVGKPGAKP
                     ETSGASTDPAAKIETEVGSYLLAEAGLNVEVALKDGALWMTVPGQPAYKLSNAGGRRY
                     KIGELPGYFATFRPAKDKAEETELYLEQPQGNFTLKKTKAADATTAPASGASAEYAGP
                     LKEVVGSYDSEVQRGRTIEVSVKDGKVFLVVPGQPPYPLSERSKDVLFSPALPDAYSF
                     GVRRDEKGAVTGLVIKQPEGEFPFKRAPEFKADLTADELMAKAIEAAGGEAAWRRHKT
                     MTATLEARLEHQGVTAEGKTYAKAPNMAAQELTLFALGKKIGSFREFFDGAAGGEEAT
                     FMPFRPKTGKALADARVAADFYGLVNWKKLFKTIEIRKLAKVGDEDAYVVVMKSEDGN
                     PVTAYFSTKTFLLLRRDTLASSGPVTQPVVEKFSDYRSVDGVMLPFTQTSTTPSNGDT
                     LIRLKEVRFDADVPDMVFKKK"
     gene            2905..3063
                     /locus_tag="AC159P_09345"
     CDS             2905..3063
                     /locus_tag="AC159P_09345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695698.1"
                     /translation="MSLLPTAYSSQTYTDYVNAVGAKRSTIALGQVIDLGGGAINGTP
                     RAKNSVTP"
     gene            complement(3410..3730)
                     /locus_tag="AC159P_09350"
     CDS             complement(3410..3730)
                     /locus_tag="AC159P_09350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695699.1"
                     /translation="MVSKKAQSKAGIPARRRGQQMGDERSNPNQQAEAEATTPALSGR
                     RKVAGKMYGDKSSRQIASDAAGPSSTSPSVSAAIPTGEPLGQSGGERAFKKRQGEKGK
                     ERRG"
     gene            complement(3842..6451)
                     /gene="lon"
                     /locus_tag="AC159P_09355"
     CDS             complement(3842..6451)
                     /gene="lon"
                     /locus_tag="AC159P_09355"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012526169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HIR3695700.1"
                     /translation="MAEENPKKEQPRKGKAEQGAPPSAQPPQPSQEARDRQQPAEGAD
                     AAQAAEGQPEVLKPEPGVFEIAVLPLQQTTLFPGTVIPLSAGRPRSVAAVEAALSTEE
                     KLLVCVTVREGRGGPEGEATPPADLYEVGTLVMVKRMMRTPDGLQLIVHGTDRVRLVE
                     WVQTDPHIRARAFTLPPPVTKDPETVEALLRNVQALIQRALAMLPEIPPEIRSAVLSS
                     QDPVQLAYFLGSILNLGVEQEQQMLEADTADELLQIAYARLTREVEIMELRSKIATEA
                     QGEMDKAQRDYILRQQMKAIQKELGEDEGGERAEAEMLRERLEQADLPDDVRKEAERE
                     LRRMERLPAAAPDYHVIRTYLEYVLELPWKKSTEDKLDLNEARRILDEDHYGLEEVKE
                     RILEFLAVVKLRRDSKSPILCFVGPPGVGKTSLGRSIARALGREFERLSLGGVRDEAE
                     LRGHRRTYIGAMPGRIIQSLRRAGVNNPVMMLDEIDKLGNDFRGDPAAALLEILDPQQ
                     NHTFRDHYLDLPFDLSKVFFIATANSLGPIPMPLRDRMEIIYIAGYSDQEKLHIARRY
                     LVPRQIAENGLQENQLEITDEALMFIAARYTREAGVRQLERTVGSVARKAALRIAQGQ
                     ALDVRVEVEDLKELLGAPRFYPEEARKELPTGVATGMAWTEMGGQLLFIEATLLPGGS
                     GLSITGQLGEVMQESARAARSYLWSHAAEFGIDAQMFKSYGAHLHVPAGAIPKDGPSA
                     GVAITSALASLMTGRRVRNDTAMTGEITLSGLVFPVGGIKEKVLAAHRAGIRRVVLPA
                     RNEPDTEDIPADVRKELEFVYANRIGEALDQTLEKLVAQPPPPAVPPQETARERAAQP
                     APAEQEPVRVRGK"
     gene            complement(6508..6888)
                     /locus_tag="AC159P_09360"
     CDS             complement(6508..6888)
                     /locus_tag="AC159P_09360"
                     /inference="COORDINATES: protein motif:HMM:NF012241.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HIR3695701.1"
                     /translation="MARSIERYLRMMPKAQGVRYTERVWYPPADVYRTRDGGWVVKVE
                     LAGVRPDEIEIAAEGEVLRVAGSRRDSTCTESVLCHQLEIVYSRFEKSIRFPCSVEGA
                     EVETLYEDGLLIIRLRVRGDEACG"
BASE COUNT         1139 a         2329 c         2276 g         1180 t
ORIGIN      
        1 ggcccgcggc ggctggagga ggggagcggt gcgtctttcg cgtctcgcac gcacgcgtat
       61 gtatacgcga gactcggccc cgcgtcacca cataagtagg tagtaaaaaa agtcgtccgg
      121 ctcgctccgg cgggcgtgta aactttcggc ccgcgcgccg cgtaaaaaca gcgacgtgga
      181 tgggagagac gctccggcca aggctgcggc ctgtgacggg gcgtctcgcg cgtcgggacg
      241 tgcggggcgt cagccttccc gcttcgacga ttcaaccagc taggagtacc aagtcatgtc
      301 cgtgtggaga cgttgcctca atgtcgcgct cgccgccgcg ctcgccgccg cgtgggcgcc
      361 gtccctcacc cttcaaagag tgtcggcgca ggccgccgcg cctgcgccgc cgcaatcgca
      421 gaccgagctt gaggcgaagc tcgccgccgc cgagaaggcg atcgaagaga agcggcggga
      481 gctgggcgtg ccgggcgccg cgctcgtcgt ggtgaaggac gaccgcgtcg tctacatgaa
      541 gggtctcggc gtgaaggact tcgagaagcg gcagccggtg acgcccgaca cgctgttcgc
      601 catcggctct tcgtcgaagg cgttcacggc gatgctcgtg gcgatggcgg cggacgcggg
      661 taagctctcg ttcgacgacc cgccgcgaaa gttcctgccc tacttcaagc tgcaagacgc
      721 ggagacggac gcccgcatca ccgtgcgcga tctgctctcg cacagctcgg gcctcaaccg
      781 cacggacatc gcgtggatca cgggaacctt gacgcgcgag gaggtgattc gcgtcgcggc
      841 gcaggcgaag ccgacggcca agctgcgcga gaagtttctt taccagaacg tgatgtacac
      901 ggcggcgggc gaggtcgcgg cgcgggccga aggctcgacg tgggagcggt tgatccgcga
      961 gcgcatcttt gggccgctcg gcatgaaggc gtccgtgctg accgcgccgg agatgcagcg
     1021 cgcgcccgac cactctttcg gctacgagta cgacgaggcg acgaaggaga cgcggcgcct
     1081 gccgcagcgc gactttccgc aggtggccgc cgcgggcgcg atcaactcga acgcgcgcga
     1141 catggccggg tggctgcggc tgatgctcgg cggcggcgcg ttcgagggca agcggctcgt
     1201 ctccgaaaag agcttcgccg aactcgtcaa gccgcagcag aagatcgcgg ggtcgatctc
     1261 ttacggcctc ggctggttcc tgcgcgactg gcacggccac aaggtggtcg agcacggcgg
     1321 caacatcgac ggcttcaacg cgcaggtcgc actgatgccc gaccagcggc tcggcttcgt
     1381 gctgctgacg aacgtgacgg cctcggcgct gcccgcgacg gcgatggagg ccgtctggtc
     1441 gaacctcgtc ggcaagcccg gggcgaagcc ggagacgagc ggcgcctcga cagaccccgc
     1501 ggccaaaatc gagaccgaag tcggcagcta cctgctcgcc gaggcgggcc tcaacgtcga
     1561 ggtcgcgctc aaggacggcg cgctgtggat gaccgtgccg gggcagcccg cctacaagct
     1621 ctcgaacgcg ggcgggcggc gctacaagat cggagagctg cccggctact tcgccacctt
     1681 ccgccccgcg aaggacaagg ccgaggagac cgagctgtat ctcgaacagc cgcaaggcaa
     1741 cttcactttg aagaagacga aggcggctga cgcgacgacc gcgcccgcct ccggcgcttc
     1801 ggcagagtac gcggggccgc tcaaggaggt cgtcggctcg tacgattcgg aggtgcagcg
     1861 gggccgcacc atcgaagtct cggtcaaaga cggcaaggtc ttcctcgtcg tgccgggcca
     1921 gccgccctac cctctgtcgg agaggtcgaa ggacgtgctc ttctcgcccg cgctgcccga
     1981 cgcctacagc ttcggcgtgc gccgcgacga gaagggcgcc gtcacgggcc tcgtcatcaa
     2041 acagcccgag ggcgagttcc ccttcaagcg cgcgcccgaa ttcaaagccg acctcaccgc
     2101 ggacgagttg atggccaaag ccatcgaggc cgcgggcggc gaggccgcgt ggcgcaggca
     2161 caagacgatg accgcgacgc tcgaagcgcg gctcgaacat cagggcgtga ccgccgaggg
     2221 caagacttac gcgaaggcgc cgaacatggc ggcgcaggag ctgacgctct tcgcgctcgg
     2281 caagaagatc ggctcgttcc gcgagttctt cgacggcgcg gcgggcggcg aggaggccac
     2341 cttcatgccc ttccgcccca agaccggcaa ggcgctggcc gacgcgcgcg tcgccgcgga
     2401 cttctacggc ctcgtcaact ggaagaagct cttcaagaca atcgagatca ggaagctcgc
     2461 gaaggtcggc gacgaggacg cctacgtcgt cgtcatgaag agcgaggacg gcaatcccgt
     2521 caccgcctac ttctcgacca agaccttcct cctgctccgc cgcgacaccc tcgcgtcgag
     2581 cggccccgtc acgcagcccg tcgtcgaaaa gttctcggac taccgcagcg tggacggcgt
     2641 catgctcccc ttcacgcaga cctccaccac gccctcgaac ggcgacaccc tcatccgcct
     2701 caaggaagtc cgcttcgacg ccgacgtgcc cgacatggtc ttcaagaaga agtaggcggt
     2761 aggcggtagg cagttaagac agagaacaga gatgaagagg cttgagaggt aaagtcccaa
     2821 gcctcttcgt tcgacaggcg ataaagtgac gaggcttgag atggaacgtc tcaagcctct
     2881 cgtcgctttt cgtctttgct gcttttgtct ttgctgccta ccgcctactc ttcgcagacc
     2941 tacaccgact acgtcaacgc cgtcggcgcc aagcgctcga ccatcgcgct gggtcaggtg
     3001 atcgacctcg gcggcggcgc catcaacggc accccccgcg ccaagaactc cgtgacaccc
     3061 tgaaagccgt gaaccggaaa ccgtgagccg tgataagtga agacagcttt cttctgatca
     3121 cggttcacgg ttcacgacct ttaacttgtc ttggctttgc aatctgttcg caaccaaagg
     3181 gggctgggcc gaggctacta tgtgcacacg taacgggcgt tccttctgag aacgagtgaa
     3241 actttggggc aggccacgac ctgagtggcc aaaggcgtca ggcttggggg agtctgacgc
     3301 cttttctttc aagaaaagta gaaaggttaa aaggcgaaaa ggaagaaaga caggctgact
     3361 tgctctgtct tttccctttt cgccttttcc ccttttaacc tttctacttt taccctcttc
     3421 gctccttccc cttttccccc tgccttttct tgaaggctct ctcgccgccg gactgtccga
     3481 gcggctcgcc ggtggggatg gcggcggaga cggacgggga ggtggaactc gggccggcgg
     3541 cgtcggacgc tatctgccgc gaagacttgt cgccgtacat cttgccggcg actttgcgcc
     3601 gaccgctcag ggcgggcgtc gtcgcctccg cctcggcctg ctggttcgga ttgctgcgct
     3661 cgtcgcccat ctgctgtccg cgccgccgcg ccgggatgcc cgccttgctc tgcgcctttt
     3721 tgctgaccat cgcccctcca gtattaatga tgaatgcaga atgcggaatg atgaatgaga
     3781 ggacaggttg aatgatgaaa agcacgctgc ttttcattca tcattccgca ctttgcattc
     3841 atcacttgcc gcgcacccgc accggctcct gttcggcggg ggcgggctgc gcggcgcgtt
     3901 cgcgcgccgt ctcctgcggc ggcacggcgg gcggcggggg ctgcgcgacc agcttttcga
     3961 gcgtctggtc gagcgcctcg ccgatgcggt tggcgtagac gaactccagc tccttgcgca
     4021 cgtccgcagg gatgtcctcg gtgtccggct cgttgcgcgc ggggagcacg acgcggcgga
     4081 tgcccgcgcg gtgcgcggcc aagaccttct ccttgatgcc gccgaccggg aagacgaggc
     4141 ccgacagagt tatctcgccg gtcatcgccg tgtcgttgcg cacccgcctg ccggtcatca
     4201 gcgaggcgag cgccgacgtg atggcgacgc ccgccgaagg gccgtccttc gggatcgcgc
     4261 ccgcgggcac gtgcaggtgc gcgccgtagg acttgaacat ctgcgcgtcg atgccgaact
     4321 cggcggcgtg cgaccagagg tacgaacgcg ccgcgcgcgc agactcctgc atcacctcgc
     4381 cgagctggcc cgtgatcgag aggcccgagc cgccgggcag cagcgtcgcc tcgatgaaga
     4441 ggagctggcc gcccatctcc gtccacgcca tgccggtcgc cacgcccgtc ggcaactcct
     4501 tgcgcgcctc ctccgggtaa aagcgcggcg cgccgagcag ctccttgagg tcttcgacct
     4561 cgacgcgcac atccagagcc tgaccctgcg cgatgcgcag cgccgccttg cgcgcgacgc
     4621 tcccgaccgt gcgctcgagc tggcgcacgc ccgcctcgcg cgtgtaacgc gcggcgatga
     4681 acatcagcgc ctcgtccgtg atttccagtt gattctcctg caagccgttc tcggctatct
     4741 gccgcgggac gagatagcgc cgcgcgatgt gcagcttctc ctgatcgctg tagcccgcga
     4801 tgtagatgat ctccatgcgg tcgcgcaagg gcatcgggat cggcccgagc gagttcgccg
     4861 tggcgatgaa gaagaccttc gagaggtcga acggcagatc gaggtagtgg tcgcggaacg
     4921 tgtggttctg ctgcgggtcg aggatttcca aaagcgccgc cgccgggtcg ccgcgaaagt
     4981 cgttgccgag cttgtcgatc tcgtcgagca tcatcacggg gttgttcacg cccgcgcgcc
     5041 gcaaagattg gatgatgcgg cccggcatcg cgccgatgta ggtgcggcgg tggccgcgca
     5101 gctccgcctc gtcgcgcacg ccgcccaaag agaggcgctc gaactcgcgc ccgagcgcgc
     5161 gcgcaatcga gcggcccaag ctagtcttgc cgacgcccgg cgggccgacg aagcagagga
     5221 tcggactctt cgagtcgcgg cgcagcttca ccacggcgag gaattcgagg atgcgctctt
     5281 tgacttcttc gaggccgtag tggtcttcgt cgaggatgcg ccgcgcctcg ttcaagtcga
     5341 gcttgtcctc ggtcgatttc ttccacggca gctccagcac gtactcaagg taagtgcgga
     5401 tgacatggta gtcgggcgcc gccgcgggca gccgctccat gcggcgcagc tcgcgctcgg
     5461 cctctttgcg cacgtcgtcg gggaggtctg cctgctccag ccgctcgcgc agcatctcgg
     5521 cctcggccct ctcgccgccc tcgtcctcgc ccagctcctt ctggatggcc ttcatctgct
     5581 ggcgcaggat gtagtcgcgc tgcgccttgt ccatctcgcc ctgcgcctcg gtggcgatct
     5641 tcgagcgcag ttccatgatc tcgacctcgc gcgtcaggcg cgcgtaggcg atctgcaaca
     5701 gctcgtccgc cgtgtcggct tcgagcatct gctgctcctg ctcgacgccg aggttgagga
     5761 tcgagccgag gaagtaggcg agctgcacgg ggtcttgcga ggagagcacg gcggagcgta
     5821 tctccggcgg tatctcgggc agcatcgcca gcgcgcgctg gatcaaagcc tgtacgttgc
     5881 ggagcagcgc ctcgaccgtc tccgggtctt tcgtcacggg cggcggcagc gtgaaagcgc
     5941 gcgcgcggat gtgcgggtcg gtctgcaccc actcgacgag gcggacacga tccgtgccgt
     6001 ggacgatgag ctggaggccg tcgggcgtgc gcatcatgcg cttgaccatg acgagcgtgc
     6061 cgacttcgta gaggtctgcg ggcggcgtgg cctcgccctc ggggccgccg cgcccctcgc
     6121 gcacggtgac gcagaccaag agcttctcct cggtcgagag cgccgcctcg accgccgcga
     6181 ccgagcgcgg gcgccccgcc gagagcggga tcaccgtgcc cggaaagagc gtcgtctgct
     6241 gcaagggcag cacggcgatc tcgaagacgc ccggctcggg cttcaacact tcgggctgcc
     6301 cctccgccgc ctgcgccgcg tccgcgccct ccgcgggctg ctgacggtcg cgcgcctcct
     6361 gcgacggctg cggcggctgc gccgacggcg gcgccccctg ctcggccttc cccttgcgcg
     6421 gctgttcctt cttcggattt tcttccgcca tgacgttttg attctctcag tcgaatatga
     6481 aagcgaggct tcggcgagat cgctttttca tccgcacgct tcgtcgcccc gcacgcgcag
     6541 gcggatgatg agcagcccgt cttcgtacag ggtctcgacc tccgcgccct cgacgctgca
     6601 agggaagcgg atcgacttct cgaagcggct gtagacgatt tcgagctggt gacagagcac
     6661 gctctccgtg cacgtcgagt cgcgccgcga ccccgcgacg cgcagcactt cgccctcggc
     6721 ggcgatctcg atctcgtcgg gccgcacgcc cgccagctcc accttcacca cccacccgcc
     6781 gtcgcgcgtc cggtacacgt ccgccggcgg ataccacacc cgctccgtgt aacgcacgcc
     6841 ctgcgccttc ggcatcatcc gcaagtatcg ttcgattgat cgcgccataa aaaaaggact
     6901 gaggactgag gactgaggac tgag
//
LOCUS       DBNGOH010000472         2192 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3370581, whole genome shotgun sequence.
ACCESSION   DBNGOH010000472 DBNGOH010000000
VERSION     DBNGOH010000472.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2192)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2192)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2192
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3370581"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(95..1114)
                     /gene="aroF"
                     /locus_tag="AC159P_09365"
     CDS             complement(95..1114)
                     /gene="aroF"
                     /locus_tag="AC159P_09365"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011984688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HIR3695702.1"
                     /translation="MLIVMKADATERDVEEVVRVISEMGLRAHPLPGPTRTAVAVTGN
                     QGPLDVSHFENLAGVAEAIRVTKPFKLISLDLRPEKTVVKVGDTSIGGDELAIIAGPC
                     AVESREQVFAVAESVKRSGAKFLRGGAYKPRTSPYAFQGLGEEGLRILAEAREQFGLK
                     IVTEALDERGVDLVERYADVIQIGARNMQNFSLLRRAGRSHLPVLLKRGLSAMLDEWL
                     LSAEYVMAEGNYNVILCERGVRTFAQHTRNTLDLAAIPAVRRESHLPVIVDPSHGTGK
                     NYMVTPLARAGVAVGADGLIIEVHDHPEKALCDGAQALTIEQYEHLVAEVRAIHDVIA
                     PAHVG"
     gene            complement(1215..>2192)
                     /locus_tag="AC159P_09370"
     CDS             complement(1215..>2192)
                     /locus_tag="AC159P_09370"
                     /inference="COORDINATES: protein motif:HMM:NF017555.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DALR anticodon-binding domain-containing
                     protein"
                     /protein_id="HIR3695703.1"
                     /translation="VGLNFYFKKFHTHADGHTVWTTTSDANEAADEAPHFGGGATVYN
                     VIDSRQTYPQDVVRRGVAAVAPEVGESASVHMSYEMVALSPAAAEELGVQLSEEERAR
                     PFIEMSGRRGLGVKSDDFIDRLEANALREVEERHPSLSVDEKRRAAHILSVGALRYFL
                     LKWTRNSVIVFDFKEALSTTGEAGPYCQYAAVRANSIFRKLPDGEPAEALRLTTAAAA
                     GGGDGAAKIAGVFEGEVGGEVWSLVMLAGRLDEAVAQSAGAAEPSHLAKYAFSLAHAF
                     NSFYNRPVNNILKEQDAARRAALVVVADFVRRQLTSALATLGIEVPERM"
BASE COUNT          329 a          723 c          759 g          381 t
ORIGIN      
        1 ttctctgcgc cctctgcgcc tctccgcgtc ctctgcgtcg agatcggcgt ttgggtaagc
       61 tcaaccgcgg gccgctaatt tctcccgccg ccaatcaccc cacgtgcgcc ggggcgatca
      121 cgtcgtggat ggcgcgcacc tcggcgacga ggtgttcgta ctgttcgatg gtaagcgcct
      181 gcgcgccgtc gcagagcgcc ttctcggggt ggtcgtggac ttcgatgatg aggccgtcgg
      241 cgccgacggc gacgcccgcg cgcgcgagcg gcgtgaccat gtagttcttg ccggtgccgt
      301 gcgagggatc gacgatgacc ggcaggtgcg actcgcgccg gacggcgggg atggcggcga
      361 ggtctaaggt gttgcgcgtg tgctgcgcga aggtgcgcac gccgcgctcg cagaggatga
      421 cgttgtagtt gccctcggcc atgacgtact cggcggacaa gagccactcg tcgagcatcg
      481 cggagagtcc gcgcttcagg agcacgggca ggtgcgagcg ccccgcgcgc cgcagcagcg
      541 agaagttctg catgttgcgc gcgccgatct gaatcacgtc ggcatagcgt tccactaaat
      601 ctacgccgcg ctcgtcgagc gcttcggtga cgatcttgag gccgaactgt tcgcgcgctt
      661 cggcgaggat gcgcagaccc tcttcgccga gtccctggaa ggcgtaaggg ctggtgcgcg
      721 gcttgtacgc gccgccgcgc aggaacttcg cgccgctgcg cttgaccgat tcggcgacag
      781 cgaagacctg ctcgcggctc tcgacggcgc aggggccggc gatgatcgcc agctcgtcgc
      841 cgccgatcga tgtgtcgccg accttgacca ccgtcttctc gggccgcagg tcgagcgaga
      901 tgagcttgaa gggcttggtg acgcggatgg cctcggccac gcccgcgagg ttctcgaagt
      961 gcgacacgtc gagcggcccc tggttgcccg tgacggcgac ggcggtgcgc gtcgggccgg
     1021 gcagagggtg cgcgcgcaaa cccatctccg agatgacgcg cacgacctct tcaacgtcgc
     1081 gctcggtggc gtcggctttc atcacgatca gcatcgaatc tcgtcctctc ttctcgcgtt
     1141 acaaagtcgg gtctacgtgc atgcccgacg aaagtcattg caagacgcgg gccgcggggt
     1201 caaccgcttc cggcttacat cctctcgggc acctcgatgc cgagcgtggc gagcgcggac
     1261 gttagctgcc tccgcacgaa atccgcgacg acgacgagcg cggcacggcg cgccgcgtcc
     1321 tgctccttga ggatgttgtt gacgggacgg ttgtagaacg agttgaaggc gtgcgccagc
     1381 gagaacgcgt atttggcaag atgcgaaggc tcggcggcgc ccgccgactg cgcgacggcc
     1441 tcgtcgagcc ttccggcgag catgacgagc gaccaaactt cgccgccgac ctctccctcg
     1501 aacacgcccg cgattttcgc cgccccgtcg ccgccgcctg ccgccgcggc ggtcgtgagg
     1561 cgaagtgcct ccgccggctc gccgtccggc aatttgcgaa agatggagtt ggcgcgcacc
     1621 gccgcgtact ggcagtaggg gccggcctcg ccggtggtcg agagcgcctc cttgaagtcg
     1681 aagacgatga cggaattgcg cgtccacttc agcaggaaat agcggagcgc gccgaccgag
     1741 aggatgtgcg cggcacgccg tttctcatcg accgacagag aagggtgtcg ctcttcgact
     1801 tcgcgcagcg cgttcgcttc gagccggtcg atgaagtcgt cggacttgac gcccaagccg
     1861 cgccgcccgc tcatctcgat gaaggggcgg gcgcgctcct cttcgctcag ttgcacgccg
     1921 agttcttcgg cggcggcggg cgagagcgcg accatctcgt aagacatgtg gacgctcgcc
     1981 gactcgccga cctcgggcgc gaccgccgcg acgccgcgac gcaccacgtc ctgcgggtag
     2041 gtctgccgcg agtcgatgac gttgtagacg gtcgcgccgc cgccgaagtg cggcgcttcg
     2101 tccgcggcct cgttcgcgtc gctcgtcgtc gtccagacgg tgtggccgtc cgcgtgggtg
     2161 tgaaacttct tgaagtagaa gttgaggccg ac
//
LOCUS       DBNGOH010000473         4521 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2086490, whole genome shotgun sequence.
ACCESSION   DBNGOH010000473 DBNGOH010000000
VERSION     DBNGOH010000473.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4521)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4521)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4521
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2086490"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1516
                     /locus_tag="AC159P_09375"
     CDS             <1..1516
                     /locus_tag="AC159P_09375"
                     /inference="COORDINATES: protein motif:HMM:NF015983.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HIR3695704.1"
                     /translation="RSAEDSHIHRHGTSNIKFGRGAVLTVDLTNRCNMMCNPCFMDAN
                     QVGYVHEPTFEDTKAILDRAVSFKPRRQIIILFSGGEPTLSPYFLDAVAYAKKVGFYR
                     ILAATNGIRYAEDIEFCKAAKAAGQHGVYLQFDGVSEEKNKHRGVGNLFDVKLRAIEN
                     LASVGIKVTLVTTIVNTINNDAIGQIVEFAAKNIDKVQTIAFQPVSFTGRDEDVPDRI
                     REQQRYTLAGMASDLDTQLGGRMKPLRDWFPLSSYSAFTSVMDMLQGADAPWGWSSCN
                     CHPNCGIFTLAVVNRHTGEFKSLFEFFDYEQFMKDVAVITDTARGKKLSYAQLGMAIM
                     RNFRPESAPEGFPVSQILNLFKPSSTSSNSDRNDRMKTQSADDPADTWRVLCVEGMWF
                     QDLFNYDFRRTEMCVIPYGTQEGEISFCAYNTGVGWRQIIEEMHKTANLADWYKEHGR
                     HAVYAKGKHVPGINAKHTLSLPIVHRILAEDADAPAAPSVTPYLVEEEEGTLVS"
     gene            1707..2240
                     /locus_tag="AC159P_09380"
     CDS             1707..2240
                     /locus_tag="AC159P_09380"
                     /inference="COORDINATES: protein motif:HMM:NF025724.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4410 domain-containing protein"
                     /protein_id="HIR3695705.1"
                     /translation="MSRLKQTIVFAALAVACLAAPVVAQDGKNVADAAEKNKYQNAEV
                     APFTAQEGVKITPEELKALTDTVVRELGEIRKFKQVLREGETPADAAAPTLKITGTVV
                     KYKAGSRAKRYLVGFGAGKSKLIADVKFIDRATGKVLYEQKADGDVVMGLFGGDSGGT
                     KSEMAEQVAKFAKKKFF"
     gene            complement(2362..2718)
                     /locus_tag="AC159P_09385"
     CDS             complement(2362..2718)
                     /locus_tag="AC159P_09385"
                     /inference="COORDINATES: protein motif:HMM:NF012722.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HIR3695706.1"
                     /translation="MRESGPQADEAGGARAEESPRTVEELRRAVEECEARLTEAADLA
                     AHVRHEINNPLTGILGQAQLLLREELSERARRRVETIVQLAARIRDTVAELREVQRAK
                     PSSAAAGDKEGEPPRR"
     gene            2848..2918
                     /locus_tag="AC159P_09390"
     tRNA            2848..2918
                     /locus_tag="AC159P_09390"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2880..2882,aa:Gln,seq:ctg)
     gene            2972..3412
                     /locus_tag="AC159P_09395"
     CDS             2972..3412
                     /locus_tag="AC159P_09395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695707.1"
                     /translation="MRAMRKLCGRCGRNRLSKFFNRRRENQLHSYCRDCHKAVNQAHY
                     LANKQAHLEKAAKRRAHLRQLINELKARPCADCGVAYPPYVMVFDHRENKRFSISQAW
                     RARSWPLVLAEIEKCDVVCANCHRERTYRHAGDVSLSTHRGKIR"
     gene            3742..4461
                     /locus_tag="AC159P_09400"
     CDS             3742..4461
                     /locus_tag="AC159P_09400"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HIR3695708.1"
                     /translation="MTKLSLALLICVAALSAPNASAAAVDTVVLFNPAAFETPESIQF
                     DRHGNAYVSLALTGEVRKIAPDGVQTTLAFMPIRPDVQPCQNGFGLPLIGGLALDHQG
                     NVYVSVTSCNAPDLGVWKVTPQGQISQLAVLPPGGAPNGIAYHGGWLYFSDSFGGAVY
                     RVHTDGNGPAQLWTNSALLAPVPLPLTPGPNGVQVFHDEVYVAVSDRAHVVAFPFFPG
                     PDPRRPSILRFHVGVAGKPRP"
BASE COUNT          824 a         1599 c         1365 g          733 t
ORIGIN      
        1 ccgctcggcc gaggactcgc acatccatcg ccacgggacc tccaacatca agttcggccg
       61 cggggccgtc ctcacggttg acctcaccaa ccgctgcaac atgatgtgca acccgtgctt
      121 catggacgcc aatcaggtcg gctacgtcca cgagccgacg ttcgaagaca ccaaagcgat
      181 tctcgaccgc gccgtctcgt tcaagccgcg ccgccagatc atcatcctct tctcgggcgg
      241 cgagccgacg ctgtcgccct acttcctcga cgccgtcgcc tacgccaaga aggtcggctt
      301 ctaccgcatc ctcgccgcca ccaacggcat ccgctacgcc gaagacatcg agttctgcaa
      361 ggccgccaag gccgccggcc agcacggcgt ctatctacag ttcgacggcg tctccgaaga
      421 gaagaacaag caccgcggcg tcggcaacct gttcgacgtg aagctgcgcg cgatcgagaa
      481 tctggcttcg gtcggcatca aggtgactct ggtgacgacc atcgtcaaca ccatcaacaa
      541 cgacgccatc ggccagatcg tcgagttcgc cgcgaagaac atcgacaagg tgcagaccat
      601 cgccttccag cccgtctcct tcacgggccg cgacgaggac gtgcccgacc gaatccgcga
      661 gcagcagcgc tacacgctcg ccggcatggc ctcggacttg gacacgcagc tcggcgggcg
      721 gatgaagccc ttgcgcgact ggttcccgct ctcgtcgtat tcggccttca cctcggtgat
      781 ggacatgctg caaggcgcgg acgcgccgtg gggctggtct tcctgcaact gccacccgaa
      841 ctgcggcatc ttcaccctcg cggtcgtcaa ccgccacacg ggcgagttca agtcgctctt
      901 cgagttcttc gactacgagc agttcatgaa ggacgtggcc gtcatcaccg acacggcgcg
      961 cggcaagaag ctctcctacg cgcagctcgg catggccatc atgcgcaact tccggcccga
     1021 gagcgcgccc gagggcttcc ccgtctcgca gattctcaac ctcttcaagc cgtcttcgac
     1081 cagctcgaac agcgaccgca acgaccgcat gaagacgcag tcggcggacg accccgcgga
     1141 cacctggcgc gtgctgtgcg tcgagggcat gtggttccag gacttgttca actacgactt
     1201 ccgccgcacc gagatgtgcg tcattcccta cggcacgcag gagggcgaga tcagtttctg
     1261 cgcctacaac accggcgtcg gctggcggca gatcatcgag gagatgcaca agaccgccaa
     1321 cctcgccgac tggtacaagg agcacgggcg ccacgccgtc tacgccaagg gcaagcacgt
     1381 ccccggcatc aacgccaagc acacgctctc gctccccatc gtgcatcgga ttctcgccga
     1441 ggacgccgac gcgcccgcgg cgccgtccgt cacgccttac ctcgtcgaag aggaagaggg
     1501 gacgctggtc tcttaaccga cacgggccgg acgccccgct cctttgcggg cggggcgttc
     1561 agccgcttcg ccgccgccaa tcaagctttc ccgtcagcgc ctgcattgca acccacaccc
     1621 gacgccgcac ttcaggtata accacaactc gccgcttccc ccacagggag tgacccaacc
     1681 cccaagtcgt agaaaggaat cagcagatga gcagattgaa gcagaccatt gtgtttgcgg
     1741 cgctggcggt cgcgtgcctc gccgcgcccg tcgtcgcgca ggacggtaag aatgtggcgg
     1801 acgccgccga aaagaacaaa tatcagaacg cggaggtcgc gcccttcacc gcgcaggagg
     1861 gcgttaaaat aacgccggag gagttgaagg cgttgaccga cacggtcgtc agggaactgg
     1921 gcgagatcag gaagttcaag caggtcttgc gtgaaggcga gacgcccgcg gacgccgcgg
     1981 cgccgacgct caaaatcacc ggcacggtgg tcaagtacaa ggcgggcagc cgcgccaagc
     2041 gctaccttgt cggcttcggc gcgggcaagt ccaagctgat cgccgatgtc aagtttatcg
     2101 accgtgcgac cggcaaagtg ctctacgagc agaaggccga cggcgatgtc gtgatgggcc
     2161 tcttcggcgg cgattccggc ggcaccaaat ccgagatggc cgagcaggtc gccaagttcg
     2221 ccaaaaagaa gttcttctga accgcgctcg acgcgcgaga cgcttcggcg tcgaagcgag
     2281 aaagagcgcc cggtcaatgg aaaccgggcg ctcttttctt tcattgcggc tcgcgcacgg
     2341 cgaacccgtt ccgcgcctcg ctcagcggcg gggcggctcg ccctctttgt cgcccgccgc
     2401 cgcggaggaa ggcttcgcgc gctgcacttc gcgcagctcg gcgaccgtgt cgcggatgcg
     2461 cgcggccagt tgcacgatgg tctcgacgcg gcggcgggcg cgctcgctca gctcctcacg
     2521 caacagcagt tgcgcctgcc cgaggatgcc ggtcagcggg ttgttgatct cgtggcgcac
     2581 gtgcgccgcg aggtccgcgg cctccgtcag ccgcgcctcg cactcctcga ccgcgcggcg
     2641 cagttcctcg accgtgcgcg gcgattcctc ggcccgcgcc ccgcccgcct cgtccgcctg
     2701 cggcccgctc tctcgcatcg ccgacgcccg acctccgcac gttttttccg ttggcatgac
     2761 tcccgcgcat tgtgcgcccc gcgccgcacc gatgacaagc tcggacgctt gacagacggg
     2821 cacgccgcgt cgaaaatact tccgcgttgc gggatggtga aatggcatca cacctgactc
     2881 tggatcaggt tttctaggtt cgagtcctag tcccgcagtt ggcgcgacgg gttctgaaac
     2941 acgcgaagtt tcagaacccg ttcgtgttat attgcgcgcc atgcggaagc tgtgcgggcg
     3001 ttgcggcagg aatcgcctct ccaagttttt caaccggagg cgcgagaacc aacttcactc
     3061 ctactgccgt gattgccaca aggctgtcaa ccaggcccac tacctcgcca acaagcaggc
     3121 ccaccttgag aaggccgcga agcgcagggc gcacctgcgg cagttgatta acgagctgaa
     3181 ggcccgcccc tgcgccgact gcggagtcgc gtatccgccg tacgtgatgg tcttcgacca
     3241 ccgcgagaac aagcgtttct caatcagcca ggcgtggcgc gcacgctcgt ggcctctggt
     3301 gctggcggag atcgagaagt gtgacgtggt gtgcgccaac tgtcatcgcg agcgcaccta
     3361 ccgtcatgcg ggcgacgttt cgctgagcac tcaccggggg aagatccgtt aagggcacgc
     3421 cgccgcgggt cgggccgata agcagacgcc cgaagatttt ccggcgcgat gaaaaaattt
     3481 cgccgatccc gcgcgcgccg tttacgcgtt tagggcaagg gaacgagacg caagcgtttt
     3541 cgctcccccg cgacgggcgg cgcgtcgaga gccgcccgcc gcctctcttt gaaacctcca
     3601 tccttcgcgc gcgcgcgtgc acggttcgcg cggcgcgtcg cactcacgga gcaggaagag
     3661 ttaccgcaac caccccgcct cggctccgaa cacccacgag ttcctcaagc ccacacaaga
     3721 aaacggagag agagaaaaaa catgacgaag ctatcgctgg ctctgttgat ttgcgtcgcg
     3781 gcgctctcgg cgccgaacgc ttcggcggcg gccgttgaca ccgtcgtcct cttcaacccg
     3841 gcggcgttcg agacgccgga gagcatccag ttcgaccgcc acggcaacgc ctacgtctcg
     3901 ctcgcgctca ccggcgaggt gcgcaagatc gcccccgacg gcgtgcagac cacgctcgcc
     3961 ttcatgccca tcaggcccga cgtgcagccg tgccagaacg gcttcggcct gccgctcatc
     4021 ggcggcctcg cgctcgacca tcagggcaac gtctacgtct ccgtcacctc gtgcaacgcg
     4081 cccgacctcg gcgtctggaa ggtgacgccg caggggcaga tttcgcagct cgccgtgcta
     4141 cccccgggcg gcgcgcccaa cggcatcgcc taccacggcg gctggctcta cttctcggac
     4201 tccttcggcg gcgccgtcta ccgcgtccac acggacggca acggcccggc gcagctctgg
     4261 acgaactccg cgctgctcgc gcccgtgccg ctgcccctga cgccggggcc gaacggcgtg
     4321 caggtcttcc acgacgaagt ctacgtcgcc gtctccgacc gcgcgcacgt cgtcgccttc
     4381 cccttcttcc ccggccccga cccgcgccgc cccagcatcc tccgcttcca cgtcggcgtc
     4441 gccggcaagc cgaggccgtg aaagacagta ggcggtaggc agcgggcagt aggcagtcaa
     4501 gacagaagca acaagaggct t
//
LOCUS       DBNGOH010000474         5065 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3186614, whole genome shotgun sequence.
ACCESSION   DBNGOH010000474 DBNGOH010000000
VERSION     DBNGOH010000474.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5065)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5065)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5065
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3186614"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..382
                     /locus_tag="AC159P_09405"
     CDS             <1..382
                     /locus_tag="AC159P_09405"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013739.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protoporphyrinogen/coproporphyrinogen oxidase"
                     /protein_id="HIR3695709.1"
                     /translation="ATLACTFSSVKFPSRAPVGHALLRAFVGGALQPEMFELDAERMT
                     TAVRRDLRDLVGVAAPPVFAHVEKWPRSMAQYTLGHLRRVSRINARLLTAPALRLAGN
                     AYEGAGVPDCVRSGETAAEFLFGG"
     gene            537..1514
                     /gene="corA"
                     /locus_tag="AC159P_09410"
     CDS             537..1514
                     /gene="corA"
                     /locus_tag="AC159P_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013955182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HIR3695710.1"
                     /translation="MSAVVNCAAYSEGRRVSTVEIEDVAHVIKEPGRFVWIGLYAPSA
                     ELLRQVQREFCLHDLAIEDAHRAHQRPKLETYGDSLFIVLRTARMNEAERRIEFGETH
                     FFVGCNFIVSVRHGSSLSYADVRARCESTPQLLRKGPGFALYAVMDSIVDQYFPVIQA
                     LEQELESVEDKIFGERSGREALEQIYQLKRQLLEVKRAISPLIDICNRLVRFDLEVIP
                     EDTRPYFRDIYDHVIRINEMVDNARELLTTALEANFSLISIGQGEVSKRFAGWAAIIA
                     VPTMVAGIYGMNFKAMPELEWAYGYPAVLLVTFGLSGLLYLLFKRSGWL"
     gene            1785..2291
                     /locus_tag="AC159P_09415"
     CDS             1785..2291
                     /locus_tag="AC159P_09415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695711.1"
                     /translation="MKSNRSGLFTKLPMLALGLFLLLPTVTVAQDGWWGQDRRERERQ
                     RREEIRERQRQRREARRERRADRRNDGYYRNDGYNSGYYGNRDNYPNYGGSYDARQTA
                     LNAGYNNGREEGREDRQRGEGFNFQDEGEFQSATKDYNSRYGDRETYRRYFREAFARG
                     YRDGYNGY"
     gene            complement(2371..>5065)
                     /locus_tag="AC159P_09420"
     CDS             complement(2371..>5065)
                     /locus_tag="AC159P_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012930170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HIR3695712.1"
                     /translation="GRPTQVTKFTDGRVLWPSISYDGRAIVFERGYGIWRMETDSGRA
                     SEVRITLRGALAGPAVERVRQTEQLQDLALAPEGKKVAFVARGEVFAASATDGGDAVR
                     VTDTAGIESRPTWTADSRKLVYVSERNGAGQLFLYDFGTNAETQLTSGTEGDTSPRFS
                     PDGKLLAFERAGRELRVLDMESKRERVVATGGLEKPPLDSDRQFVWSPDSKWIAYMPV
                     GSKLFRNVHVAPIEGDGKGQPVSFLANVGSNSVPWSPDGTYILFDTGQRTESGQLARI
                     DLLPRTPRFREDQFRELFKEETPKSVTPSLRRQETQTQAPAPSRDTPTTAPATPTPTP
                     TLTPTPSTTPTPIPPTVDQVFMPTPTPTPATTPAPGQAAGRGASADEQKKGKPVEVVF
                     EGIRRRLSMLPVGVDVFFHTISPDGKYVLMIAGAVNQTNLYLYPLDELAREPAVARQL
                     TSTATFKSAAQFTPDSREVFYLEGGRIQVAPLDQRQPGRPLAVTAEMDVDFAREKFEV
                     FEQGWEYMRDNFYDPAYHGVDWQAARAEYRPFIAGAQTPDEMRRLMRLMVGELNASHL
                     GVSAPFGGAAAFTTGRLGLGFDREEYERAGRLRVTEVVPLGPAALARDSAAPERARAI
                     GVGEYLLAVDGRAIDARTNLDELLSFKVNRRVVLTVAARADGGEKKELEVRPVNVGTE
                     KGLRYRKWVDERRAYVQRASGGRLGYVHMYDMSSASLAQLFVDLDAENQSREGVVVDV
                     RHNDGGFVNVYAIDVLARRSYLRMTPRGFAASPARTVLGQRALELPTVLVTDQHTLSD
                     GEDFSEGYRALGLGKIVGEPTAGWIIYTTNLRLVDGTVFRIPFIKVETSAGVNMERNP
                     RPVDIAVERPIGESYTERDAQLDAAVRQLLKDKGEQTSGSK"
BASE COUNT          811 a         1761 c         1656 g          837 t
ORIGIN      
        1 cgcgacgctg gcctgcacct tcagcagcgt caagttcccc tcccgcgcgc ccgtgggtca
       61 cgccctgctg cgcgccttcg tcggcggggc gctccagccc gagatgttcg agcttgacgc
      121 agagcgcatg acgacggccg tccgccgcga cctccgcgac ctcgtcggcg tcgcggcgcc
      181 gcccgtcttc gcgcacgtcg agaagtggcc gcgctcgatg gcgcagtaca ccctcggcca
      241 cctccgaagg gtcagccgca tcaacgcacg tttgctgacc gcgcccgcgc tccgcctcgc
      301 cgggaacgcc tacgaaggcg cgggcgtccc cgactgcgtc cgcagcggcg agaccgccgc
      361 cgagttttta ttcggcggct aagccccgcg tgcagccgtg ctatcatgcc cgtcgccgcc
      421 tttcgtcttc gaccaactcg tacccggcga ggagaacctt gaagaagatc acctccgaga
      481 gagcctcgag cgacatcgcg tcggacgccg aagcgcactg gacggtggtg ccgggcatga
      541 gcgccgtcgt caattgcgcg gcctacagcg agggccgccg cgtctcgacc gtcgagatcg
      601 aagacgtggc tcacgtcatc aaagagccgg gccggttcgt ctggatcggc ctctacgcgc
      661 cgagcgccga gctgctgcgg caggtgcagc gcgaattctg tctgcacgat ctcgccatcg
      721 aggacgcgca ccgcgcgcac cagcggccca agctcgaaac ctacggcgac tcgctcttca
      781 tcgtgctgcg caccgcgcgg atgaacgagg ccgagcgccg catcgagttc ggcgagacgc
      841 acttcttcgt cggctgcaac ttcatcgtct cggtgcgcca cggctcgtcg ctttcctacg
      901 ccgacgtgcg cgcccgctgc gagagcacgc cgcaactgct gcgcaagggg ccgggcttcg
      961 cgctctacgc cgtgatggac tccatcgtcg atcaatactt ccccgtcatt caggcgctcg
     1021 aacaggaact cgaatcggtc gaagacaaaa tcttcggcga gcgttcgggc cgcgaggcgc
     1081 tcgaacagat ttaccagctc aagcgccaac tgctcgaagt caagcgcgcc atctcgccgc
     1141 tcatcgacat ctgcaaccgc ctcgtccgct tcgacctcga agtcatcccc gaagacacgc
     1201 gcccctactt ccgcgacatc tacgaccacg tcatccgcat caacgagatg gtggacaacg
     1261 cgcgcgagct gctgacgacg gcgctcgaag ccaacttctc actcatctcc atcgggcagg
     1321 gcgaagtctc gaagcgcttc gccgggtggg ccgccatcat cgccgtcccg acgatggtcg
     1381 cgggcatcta cgggatgaac ttcaaggcga tgcccgagct ggaatgggct tacggctacc
     1441 ccgccgtgct gctcgtcacc ttcggcctct cgggcctgct ctacctgctc ttcaagcgct
     1501 ccggctggct ctgaacgccg cagcctccgt tatccgtttg ccgctcagtt tgcccgccat
     1561 atttccgtcc atcggacaag acaccgcgat acggccccgc gtgtacccca taaatacacg
     1621 ttgacccctt atcgtccgtc gcggcgccgc gcattcacga caagtcaacc gcccttcaac
     1681 ggtttacacg ggcgcgccct actggcacgg ttcttgtaac cactggaagt gagattacga
     1741 cgcgaagtcg aggacgcttg aaagcacaag gaggaaggtt caagatgaaa agcaatcgta
     1801 gcggtctgtt caccaaactc cccatgctcg cactcggcct gttccttctg ctgccgaccg
     1861 tgacggtcgc gcaggacggt tggtggggtc aggatcggcg cgagcgcgaa cgccagcgtc
     1921 gtgaggagat acgtgaacgc caacgccagc gccgcgaggc gaggcgcgag cgccgcgccg
     1981 accgtcgcaa tgacggttac tatcgcaacg acggttacaa cagcgggtac tacggcaacc
     2041 gggacaacta tcccaactac ggcggctcgt acgacgcgcg ccagacggcg ctgaacgcgg
     2101 gctacaacaa cggtcgtgag gaaggccgcg aagaccgcca gcgtggcgag ggcttcaact
     2161 tccaggacga gggcgagttc cagagcgcga cgaaggacta caactcgcgc tacggcgacc
     2221 gcgagaccta ccgacgttac ttccgcgagg ctttcgcgag gggctacagg gacggctaca
     2281 acggttacta aaaccagccg acctgtatcg aacaaaagcg tgcgacggac tcattccgtc
     2341 gcacgctttt ttcgtcaggg gccgggcctc tcacttgctg ccgctcgtct gctccccttt
     2401 atctttcaac aactgcctga cggcggcgtc gagctgcgcg tcgcgttcgg tgtagctctc
     2461 gccgatgggt cgctcgaccg cgatgtccac ggggcgcggg ttgcgttcca tgttgacgcc
     2521 cgcgctcgtc tcgaccttga tgaaggggat gcggaagacc gtgccgtcca cgagtcgcag
     2581 gttcgtcgtg tagatgatcc agccggcggt cggctcgccg acgatcttgc cgaggccgag
     2641 cgcgcggtag ccttccgaaa agtcctcgcc gtccgagagc gtgtgctgat ccgtgacgag
     2701 caccgtcggg agttcgagcg cgcgctggcc gagcaccgtg cgcgccggcg aggcggcgaa
     2761 gccgcgcggc gtcatccgca aatagctgcg gcgcgcgagc acgtcgatgg cgtagacgtt
     2821 gacgaagccg ccgtcgttgt ggcgcacgtc cacgaccacg ccctcgcgcg actggttctc
     2881 cgcgtcgagg tctacgaaga gctgcgccag cgaggccgaa gacatgtcgt acatgtgcac
     2941 gtagccgagg cggcccccgc tcgcgcgctg gacgtaagcg cgcctctcgt caacccactt
     3001 ccggtagcgc agacccttct ccgtgccgac gttgacgggc cgcacctcca gctccttctt
     3061 ctcgccgccg tccgcgcgcg ccgcgaccgt gagcacgacg cggcggttaa ctttaaagct
     3121 cagcagctcg tcgaggttcg tccgcgcgtc gatggctctg ccgtccaccg cgagcaggta
     3181 ttcgccgacg ccgatggcgc gcgcgcgctc gggcgccgcc gagtcgcgcg ccagcgcggc
     3241 ggggccgagc ggcactacct ccgtcacgcg caggcggccc gcgcgctcgt actcctcgcg
     3301 gtcgaagccg aggccgagcc tgccggtcgt gaacgccgcc gcgccgccga acggtgcaga
     3361 gacgccgagg tgcgaggcgt tcagctcgcc gaccatgagg cgcatcaggc ggcgcatctc
     3421 gtcgggcgtc tgcgcgcccg cgatgaaggg ccggtactct gcgcgcgcgg cctgccaatc
     3481 gacgccgtgg taggccgggt cgtagaagtt gtcgcgcatg tactcccagc cctgctcgaa
     3541 gacctcgaac ttctcgcgcg cgaagtcaac gtccatctcg gcggtgacgg cgagcgggcg
     3601 gcccggctgc cgctggtcga gcggcgcgac ctgtatgcgc ccgccttcga ggtagaagac
     3661 ctcgcggctg tcgggcgtga actgcgcggc gctcttgaag gtcgccgtcg aagtgagctg
     3721 gcgcgcgacc gccggctcgc gcgccagctc gtcgaggggg tacagataaa ggttcgtctg
     3781 attgacggcg cccgcgatca tcagcacgta cttgccgtcg gggctgatgg tgtggaagaa
     3841 cacgtccacg ccgaccggca gcatgctcag gcgtcggcgg atgccctcga agacgacctc
     3901 gaccggcttc cctttcttct gctcgtccgc cgaagcgccg cggcccgccg cctgtccggg
     3961 ggccggagtc gtcgcaggcg taggcgtggg cgtcggcatg aatacctgat cgacggtggg
     4021 aggtatcggc gtcggcgttg ttgaaggcgt cggcgtgagc gtcggcgtcg gagtcggcgt
     4081 cgcgggcgcc gtggtcggcg tgtcgcgcga aggcgcaggg gcttgcgtct gcgtctcctg
     4141 ccggcgcagc gaaggcgtga ccgacttcgg cgtctcctcc ttgaacagct cgcggaactg
     4201 gtcttcgcgg aagcgcggcg tgcgcggcaa aaggtcgatg cgcgcgagct ggcccgactc
     4261 cgtgcgctgt cccgtgtcga agaggatgta ggtgccgtcg ggactccaag ggacgctgtt
     4321 gctgccgacg ttggcgagga agctcacggg ctgtcccttg ccgtcgccct cgattggcgc
     4381 gacgtggacg ttgcgaaaca gcttgctccc gaccggcatg tacgcgatcc acttcgagtc
     4441 cggcgaccac acgaactgcc ggtcggaatc gagcggcggc ttttccagac cccccgtcgc
     4501 cacgacgcgc tcgcgcttcg actccatgtc gagcacgcgc agctctcggc ccgcgcgctc
     4561 gaacgcgagg agcttgccgt ccggcgagaa gcgcggcgac gtgtcgccct cggtgccgct
     4621 cgtcaactgc gtctcggcgt tcgtcccgaa atcgtagaga aagagctgcc ccgcgccgtt
     4681 gcgctccgac acgtagacga gcttgcggct gtccgccgtc cacgtcgggc gcgactcgat
     4741 acccgccgtg tcggtgacgc gcacggcgtc gccgccgtcg gtcgccgaag cggcgaagac
     4801 ttcgccgcgc gcgacgaagg cgaccttctt gccttcgggc gcgagcgcga ggtcctgcaa
     4861 ctgttcggtc tgccgcacgc gctcgacggc cggcccggcg agcgcgcccc ggagcgtgat
     4921 gcgcacttcg gaggcgcgcc ccgagtcagt ctccatgcgc cagatgccgt agccgcgctc
     4981 gaagacgatg gcgcgcccgt cgtaagagat gctcggccac agcacgcgcc cgtcggtgaa
     5041 cttcgtcacc tgcgtggggc gcccg
//
LOCUS       DBNGOH010000475         4651 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_5019544, whole genome shotgun sequence.
ACCESSION   DBNGOH010000475 DBNGOH010000000
VERSION     DBNGOH010000475.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4651)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4651)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4651
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_5019544"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(192..267)
                     /locus_tag="AC159P_09425"
     tRNA            complement(192..267)
                     /locus_tag="AC159P_09425"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(231..233),aa:Glu,seq:ttc)
     gene            600..2462
                     /locus_tag="AC159P_09430"
     CDS             600..2462
                     /locus_tag="AC159P_09430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695713.1"
                     /translation="MKRLLLLVTLLSVPLGHAAAQTPPAAEPQASPQSEKEKAEARAE
                     LEKKALVMLEQTIGDAMSLRVPENRVRVLAGAADLLWPRDEKRARSLFRDALLSLNEA
                     SANSPRGREQRHRAYWTSRAVRQEALHLVARRDPQLALELLQATRQGQPPPDPHDSTP
                     TDFHQPDHELHLEHGLLSQVAANDPKRALQLATESLSKGYSVELLNLVRRLNEKDAEA
                     ATKFAGEVARRLRAENLLRNREALHVAAQFLQSTAPRRQTMVTVGAAGGGWAGGKPLA
                     LDEQTRRDLVDALVSAALAAAPAEGYYRHYYLFSTLRSTMAEVEKLAADRAPLVRRRM
                     AEFEQTLGSANRKWADYEQLLRQDSPDALLEAAAKVPAEMRPAFYSSAAFAALGAGDA
                     ERARKIVGDNFSGGQRTQMLELLDHRLLLDSLKQGKVEEAKNFVARIRDDEKRAGALA
                     KLALWAASKGDRKTARLLLAEAREIVGERPRNLQQLEAHLQLARAYALVEPPRAFEIV
                     EMAVDRANDMIAAADVLDGFLGGPEIFRDGELLMPAGVASLETIFEQYGRQLAELARA
                     DFDRAKAAASRFSRVEVRAMARLLVAQGLLSDRQPPEPTPEALMSAGITQPRNF"
     gene            2584..4473
                     /locus_tag="AC159P_09435"
     CDS             2584..4473
                     /locus_tag="AC159P_09435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="coiled-coil domain-containing protein"
                     /protein_id="HIR3695714.1"
                     /translation="MLTKAVCLALSLLLFPATSAAQVKQRRAAPPERKEDAESSRAES
                     ELEEKAVGLLEETVREADALKLAENRVHVQMVAAGLLWPRDADAARALFKQATDGVAA
                     MIAEGNPNDPQYQNERHVISQLRQQLVLATAAHDAKLALDFLRATRQPPPFAYADAEH
                     RQADQELHLENLLASQVAAQDTKQALKMAEESLGRGVSQGLTNVFHQLAAKDKEAAAR
                     LATEIIKKLRAEDLTRDYEASGVALHLLTQTRGEAAVVAEPAAPSPPAPNVVYSRGGG
                     WHGSGAQVVIEERWRRQLVETLAAAALSEPSPRHSGVVQNLLSMLQQSLPEVERYAPA
                     RVAALKRRAATENRADPRGAAWREHNDLVQNGTIEAMLEAAPKVPPEVRDQLYHNAAW
                     KAVNENNIERARQIAENIANPERRAQTLKEMEWQHGVRAAEQGNIHDARRLIARSATV
                     DEKVQLLLQLTSTAQGRGDKATARELLEEARALVGRGENAQQFYALFGVARAYAALDE
                     GVSYEMIETAIDRLNELMAAAVVVNGFGQDAFREGELRQQGGYMWNDLVGRCAQELAA
                     LAPKNFERSRSLAQRFQRTDARTTAQLLFAQNVLQSITQRNNTNTRRIQNLPLNSRTI
                     IINEH"
BASE COUNT          822 a         1593 c         1614 g          622 t
ORIGIN      
        1 aaccgaccgc gaggcgcggc ttcggcctgc ggcaggccgg acggagatac aaagcgttgg
       61 aaatttctcg taattctgga cggggaagca agtgccgcgc tgttgcccga gagttgaggg
      121 aacgcgaccg ccgaggtatc catcaacgaa aacagccgcc ccagaggcgg ctgtaacacg
      181 tcgccaaggt gtggcgtccc gtacgggagt cgaacccgtg tcgccgccgt gaaagggcgg
      241 tgtcctaggc cactagacga acgggacttg ctgattcgcc ggaaaacgtt ggcgataaag
      301 ttaccgcagc gcctgtggag tgtcaaaccg cgcccgcgcc gcgaggggcc ttggccgaac
      361 cggccttcga tgatggcggg cgcgaatgat gacggcgggc gctaaaaagt gtgcccgcgc
      421 cgaggggtaa agtggtaaga ataagggccg cgggcgcgca tccttttggc gcgcggcggg
      481 catcgcagca gcacgcgtcc cgcgtgtttc cggatcaaag gccggctcgc cgcgtttaat
      541 cgtcagcgtg catcgcaggc atcgccgaag cagctttgtg agtggaggcc ggaatgaaga
      601 tgaaacggtt gctcttgctc gtcacgctcc tctccgtgcc gctcgggcac gccgccgcgc
      661 agacgccgcc cgccgcggag ccgcaagcct ctccgcagag cgagaaggag aaggcggagg
      721 cgcgggccga gcttgagaag aaggccctcg tcatgctcga acagaccatc ggcgacgcga
      781 tgagtttgcg cgtcccggag aaccgcgtgc gcgtgctcgc gggcgcggca gacctgttgt
      841 ggccgcgcga cgagaagcgc gcgcgctcgc tcttccgcga cgcgctgctc tccttgaacg
      901 aggcgtcggc gaactcgccg cgcgggcgcg agcagaggca ccgcgcctac tggacttcgc
      961 gcgccgtcag gcaggaggct ttgcacctgg tcgcgcgccg cgacccgcag ctcgcgctcg
     1021 agctcctaca ggcgacgcgg caggggcagc cgccgcccga cccgcatgac agcacgccca
     1081 ccgacttcca tcagcccgac cacgaactgc acctcgaaca cggcctgctc tcgcaggtcg
     1141 cggcgaacga cccgaagcgc gcgctgcaac tggcgaccga aagcctctcg aagggttact
     1201 cggtcgagct gttgaacctc gtgcggcggc tcaacgaaaa ggacgcggag gcggcgacga
     1261 agttcgcggg cgaggtcgcg cgccggctcc gcgccgagaa cctgctgcgg aatcgggagg
     1321 cgctgcacgt cgcggcgcag ttcctccaat cgacggcgcc gcggcggcag acgatggtga
     1381 cggtcggcgc cgccgggggc ggctgggcgg ggggcaagcc gctcgcgctc gacgaacaga
     1441 cgcggcgcga cctcgtggac gcgctcgtgt cggcggcgct cgccgccgcc cccgccgaag
     1501 gctactaccg tcactactat ctcttctcga cgctgcgctc gacgatggcc gaagtggaga
     1561 agctggccgc cgaccgcgcg ccgctcgtgc gccgccggat ggccgagttc gagcagacgc
     1621 tcggctcggc gaacaggaag tgggccgact acgaacagct cctgcggcag gactcgcccg
     1681 acgcgctgct cgaagccgcc gcgaaggtgc ccgcggagat gcgtccggct ttctattcga
     1741 gcgcggcgtt tgcggcgctc ggcgcgggcg acgccgagcg cgcgcgcaag atcgtcggcg
     1801 acaacttcag cggcgggcag cgcacgcaga tgcttgagct gctcgaccac cgcttgctcc
     1861 tcgactcgct gaagcagggc aaggtcgagg aggcgaagaa tttcgtcgcg cgcatccgcg
     1921 acgacgagaa gcgcgctggc gcgctcgcca agctcgcgct gtgggccgcc tcgaagggcg
     1981 accggaagac ggcgcggctg ctcctcgccg aggcgcgcga gatcgttggc gagcgcccgc
     2041 gcaatctcca acaactcgaa gcgcacctcc agctcgcgcg cgcctacgcg ctcgtcgagc
     2101 cgccacgcgc cttcgagatc gtcgagatgg cggttgaccg cgccaacgac atgatcgcgg
     2161 cggcggacgt gctcgacggc ttcctcggcg ggccggaaat cttccgcgac ggcgagctgt
     2221 tgatgcccgc gggcgtcgct tcgttggaga ccatcttcga gcagtacggc aggcaactgg
     2281 cggagctggc gcgcgcggac ttcgaccgcg cgaaggccgc cgcctcgcgc ttcagccgcg
     2341 tcgaggtgcg cgcgatggcg cggctgctcg tcgcgcaagg gctgctctcc gaccgtcagc
     2401 cgcccgagcc gacgcccgag gcgctgatga gcgcgggcat cacgcagccg cggaactttt
     2461 aagcgcccgc gcggcgagcc gctcggaacg cgtgagacga gccgcggcgc aagtcgcccc
     2521 gcgccccgtc gttctgaagg cagtcgcctc cacgaccgaa gaactttaca aggggacatc
     2581 cggatgttaa cgaaagcagt ctgcctcgcc ctctcgctgt tgctcttccc ggccacgtcc
     2641 gccgcgcagg tgaagcagcg ccgcgccgcg ccgcccgaac gcaaagagga cgcggagtcg
     2701 tcgagggccg agagcgagct tgaggagaag gcggtcggcc ttctggaaga gaccgtgcgc
     2761 gaggccgacg ccctgaagct cgcggagaac cgcgtgcacg tgcagatggt cgcggcgggc
     2821 ctgctctggc cgcgcgacgc cgacgccgcg cgcgccctct tcaagcaggc gaccgacggc
     2881 gtcgccgcga tgatcgcgga gggcaacccg aacgacccgc agtatcagaa cgaacggcac
     2941 gtgatctcgc agctccgcca acagctcgtc ctcgccaccg ccgcgcacga cgccaagctc
     3001 gcgctcgact tcctgcgcgc gacgcgccag ccgccgccct tcgcctacgc cgacgcggag
     3061 cataggcagg ccgaccagga gttgcacctg gagaacctcc tcgcctcgca ggtcgccgcg
     3121 caggacacga agcaggcgct caagatggcc gaggagagtc tgggccgcgg cgtgtcgcag
     3181 gggttgacca acgtgttcca ccagctcgcc gcgaaggaca aggaggcggc ggcgcggctg
     3241 gccacggaga tcatcaagaa gctgcgcgcg gaagacctga cgcgcgacta cgaggcgtcc
     3301 ggcgtcgcgc tccacctgct cacgcagacg cgcggcgagg cggcggtcgt cgccgaaccc
     3361 gccgcgccgt cgccgccggc gccgaatgtc gtctacagca gaggtggggg ctggcacggc
     3421 tccggcgcgc aggtcgtcat cgaagaacgt tggcggcggc agttggtcga gacgctcgcc
     3481 gcggcggcgc tgtcggagcc gtccccgcgc cacagcggcg tcgtgcagaa cctcctctcg
     3541 atgcttcagc agtcgctccc ggaagtcgag cggtacgcgc ccgcgcgcgt cgcggcgctc
     3601 aaacggaggg ccgcgaccga gaaccgcgcc gacccgcgcg gcgcggcgtg gagggaacac
     3661 aacgacctcg tccagaacgg caccatcgag gccatgctcg aagccgcacc gaaggtcccg
     3721 cccgaggtgc gcgaccagct ctaccacaac gccgcgtgga aggccgtgaa cgagaacaac
     3781 atcgagcgcg cccgccagat cgccgagaac atcgccaacc cggagcggcg cgcgcagacg
     3841 ctcaaggaga tggaatggca gcacggcgtg cgcgcggccg agcagggaaa catccacgac
     3901 gcgcgccggt tgatcgcgcg ctccgcgacc gtggacgaga aggtgcaact tctcctccag
     3961 ctcacttcga cggcgcaggg ccgcggcgac aaggcgacgg cgcgcgagct gctcgaagag
     4021 gcgcgcgcgc tggtcgggcg gggcgagaac gcgcagcagt tctacgcgct cttcggcgtc
     4081 gcgcgcgcct acgcagcgct cgacgagggc gtctcgtacg agatgatcga gacggccatc
     4141 gaccggctca acgagctgat ggcggcggcg gtcgtggtca acggcttcgg gcaggatgct
     4201 ttccgcgagg gcgagctgcg gcagcagggc ggctacatgt ggaacgacct cgtcgggcgc
     4261 tgcgcgcagg agctggccgc cctcgcgccc aagaacttcg agcgctcgcg ctcgctcgcg
     4321 cagaggttcc agcgcaccga cgcgcgcacg accgcgcaac tcctcttcgc gcagaacgtc
     4381 ttgcagtcca tcacacagag gaacaacacg aacacgaggc gcatccaaaa cctcccgctc
     4441 aacagccgca cgataatcat caacgagcac tgagaagtag ttggcagtag gcagtaggca
     4501 gtaggcagta aaagcaagag gcttgagagt caacgtctca agcctcttgc tttgttgctt
     4561 gaagagcgaa gaggcttcgg actttacctc tcaacaatcc tcgctctttt gtctttactg
     4621 cctactgcct actgcctacc gcctactctc t
//
LOCUS       DBNGOH010000476         2776 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3553412, whole genome shotgun sequence.
ACCESSION   DBNGOH010000476 DBNGOH010000000
VERSION     DBNGOH010000476.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2776)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2776)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2776
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3553412"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..858
                     /locus_tag="AC159P_09440"
     CDS             <1..858
                     /locus_tag="AC159P_09440"
                     /inference="COORDINATES: protein motif:HMM:NF016777.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR3695715.1"
                     /translation="VMIKATASLLIIDAHCHAGKGDGLSGPWDTSAPLEQYLRRAARA
                     GIRRTVLLAAFHSDYRAANREVARIVASRPDRFYGFAFVHAERDRGRVFQMVREAVEN
                     YGFRGIKLHRHDARITREVCEVARAFRLPVIYDVMGEVTVAELLADEYPDVPFIIPHL
                     SSFADDWRSQLALIDHLARHPNIYTDTSGVRRFDLLEQAVGRAGARKVLFGTDGPWLH
                     PEVELHKIRMLRLKEEDERLVLGENLLRLMARGGGARVTPPPPEPDAVTPTPPAPRDP
                     WGGPQFPGP"
     gene            complement(944..2374)
                     /locus_tag="AC159P_09445"
     CDS             complement(944..2374)
                     /locus_tag="AC159P_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015347442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HIR3695716.1"
                     /translation="MIETKFWYDSLPGDGAATAYIQAALEQLRGVGLVPLAVDRGDPP
                     RGVSVVFFDAVGPKLFDLLRAASRNGSAPVLAVARARAALGDNAWRLLQAGASDVLVW
                     EGAGDPAGDVAARVERWEQIGRVVDSPLVKDNLVGESGVWRALLRRVVEAALFSDAPL
                     LLIGESGTGKELLARLIHTLDRRPDKRDLITLDCTTVAPELAGSEFFGHERGAFTSAV
                     AARDGAFALADGGTLFLDEVGELPLRLQAELLRVVQEHTYKRVGSNVWQKTNFRLICA
                     TNRDLEEASARGDFRLDFYYRIAGWVFRLPPLRERAGDILPLACNFMRRHRPGVEPPE
                     FDEPVRDYLLRRDYPGNVRDLKQLMTRIMTSHVGPGPVTVGDIAENERPCVADEQRDW
                     RDVFFEDSVRRALALGHGLKEITSAAADTAIRLAIEDEEGRGRLKRAAQRLGVTERAL
                     QMHRATRREVNAVGAEGGEANSNGDA"
BASE COUNT          438 a          967 c          925 g          446 t
ORIGIN      
        1 gtgatgatta aggccacagc gtcgctgctg attattgacg cccactgtca cgccgggaaa
       61 ggcgacgggt tgagcggccc ttgggacacg tctgcgccgc tcgaacaata cttgcgccgt
      121 gcggcccggg cgggtatccg gcgcacggtt ctgctcgcgg ccttccactc cgactaccgc
      181 gccgccaacc gcgaagtggc gcgcatcgtc gccagccgcc ccgaccgttt ctacggcttc
      241 gccttcgtcc acgccgagcg cgatcgcggg cgcgtctttc aaatggtcag ggaggcggtc
      301 gagaactacg gcttccgcgg gatcaagctg caccgccacg acgcccgcat cacgcgcgag
      361 gtctgcgaag tcgcgcgcgc cttccggctg cccgtcatct acgacgtgat gggcgaggtg
      421 accgtggccg agctgctcgc cgacgagtac ccggacgtgc ctttcatcat cccgcacctg
      481 agtagcttcg ccgatgactg gcgatcgcaa ctggccctca tcgaccacct cgcgcggcac
      541 cccaacattt acacggacac ttcgggcgtc cgccgcttcg acctgttgga gcaagccgtc
      601 ggtcgtgcgg gggcgcgcaa agtgctcttc ggcaccgacg gcccctggct gcacccggaa
      661 gtcgagctgc acaagattcg gatgctccgg ctcaaggagg aggacgagcg gctggtgctc
      721 ggcgaaaacc tgctccggct gatggcgcgc ggcggcggcg cgcgggtgac gccgccgcct
      781 cccgaaccgg acgcggtcac gcccacgccc cccgcgccgc gcgatccatg gggcggcccg
      841 cagttccccg gcccataaac tccgttcgtc acgaaatcgc ccgtctggcg gggcgagtct
      901 ttcggctatc gacgggttga agtcgaaatc caacgcgacc taatcaggcg tcgccgttcg
      961 agtttgcctc cccgccctcc gcgccgacgg cgttgacctc gcgccgcgtt gccctgtgca
     1021 tttgcagggc gcgttcggtc acaccgagcc gctgcgcggc gcgcttcaac ctgccgcggc
     1081 cttcctcatc ttcgattgcc agacggatgg ccgtgtcggc ggcggcgctc gtgatctcct
     1141 tcaagccgtg gccgagcgcg agcgcgcgcc tgaccgaatc ttcgaagaac acgtcgcgcc
     1201 aatccctctg ctcgtcggcc acacacgggc gctcgttctc ggcgatgtcg ccgacggtaa
     1261 ccgggccggg gccgacgtgg ctcgtcatga tgcgcgtcat cagttgcttg aggtcgcgca
     1321 cgttgccggg gtagtcgcgc cgcagcaggt aatcgcgcac gggttcgtca aactccggcg
     1381 gctccacgcc ggggcgatga cgccgcatga aattgcatgc gaggggcagg atatcgccgg
     1441 cgcgttcgcg cagagggggg agccggaaga cccatccggc gatccggtag tagaagtcga
     1501 gccggaagtc cccgcgcgcc gacgcctcct ccaggtcgcg gttcgtcgcg cagatcagcc
     1561 ggaagttggt cttctgccag acgttgctgc cgacgcgctt gtaggtgtgt tcctgcacca
     1621 cgcggagcag ttcggcctgc aagcgcaggg gcaactcgcc cacctcgtcg aggaacagcg
     1681 ttccgccgtc ggcgagcgcg aacgcgccgt cgcgcgccgc gaccgcgctg gtgaaggcgc
     1741 cgcgctcgtg cccgaagaac tcgctcccgg ccagttccgg cgccacggtc gtgcagtcga
     1801 gcgtgatgag gtcgcgctta tccgggcgcc ggtcgagcgt gtggatcaga cgcgccaaca
     1861 gctccttgcc cgtaccgctc tcgccgatga gcagcaacgg ggcgtcggag aacagcgccg
     1921 cctctacgac gcgccgcagc agagcgcgcc agaccccgct ctcgccgacc aagttatcct
     1981 tgacgagcgg ggagtcaacc acgcgtccga tctgttccca gcgctcgacg cgcgcggcca
     2041 cgtcacccgc cgggtcgccc gcgccttccc agacgagcac gtccgacgcc ccggcttgca
     2101 gcaagcgcca ggcgttatcc cccagcgccg cgcgcgcgcg cgccaccgcc agcacgggcg
     2161 ccgagccgtt gcggctggcg gcgcgcagca ggtcgaacag tttcgggccg accgcgtcga
     2221 agaagacgac gctgacgccg cggggcgggt cgcccctgtc caccgccaag ggcacgaggc
     2281 cgacgccccg cagctgctcc agcgccgcct gtatgtaggc cgtggcagcg ccgtcgcccg
     2341 ggagactgtc ataccagaat ttcgtctcga tcacggtgta cctcaccgtg tcgcgcagga
     2401 ttcgtcgacc gtgtgacgga ggagtatccg catgacgcga atccttccga cgttatctaa
     2461 cgacagcttg ccgcgtgaaa tcgaggcggg ccagcggagc gcccgccgac acgtcaatca
     2521 agctctgggc tttatgagtc ggggtttgcg gccttaactg agcgtcacgc ctgcggttgc
     2581 gtgtggagac gaggggctat gcccccgaga tgtcacccga tggggcgatg atggccgggc
     2641 ggtctgaaga cttgacagtt cgaagccgta tcgcgtgggg gggtagcgcc ggctccgcat
     2701 tgtggctatg agtaactaac gagataggtg gcgggagcac catgacctga cctcctgacg
     2761 ggaaagcgcg cgagaa
//
LOCUS       DBNGOH010000477         4756 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1904569, whole genome shotgun sequence.
ACCESSION   DBNGOH010000477 DBNGOH010000000
VERSION     DBNGOH010000477.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4756)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4756)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4756
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1904569"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            238..885
                     /locus_tag="AC159P_09450"
     CDS             238..885
                     /locus_tag="AC159P_09450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695717.1"
                     /translation="MYSMVALAAPQQGPTGDLSVVGEVTVNGTSAISGATVFSDSTVT
                     TAKGSSAVVSLGKLGRVEVLPESSLKLSFTDTGVTAMLDQGRVRVSSSSGVAASVTTK
                     DGSAVADNSQPNVFMVDVECGNTVVSTQSGRVELRAGNSVKQIAAGSQDTAGQATPGT
                     RCTRFSQPDMRGLGGGALAALLLAAGGAIAAAIFAASSDDEFNFGDGPIVISPSR"
     gene            1042..2340
                     /locus_tag="AC159P_09455"
     CDS             1042..2340
                     /locus_tag="AC159P_09455"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose dehydrogenase family protein"
                     /protein_id="HIR3695718.1"
                     /translation="MHIAVIGTGYVGLVTGACFAEFGVNVTCVDVDEEKIKQLAEGKI
                     PIYEPGLDQLVARNAQAGRLHFTTDIRLAVEQALVIFLAVGTPPREDGSADLRFIEEA
                     ARSVADHMNGYKVIVTKSTVPVGTGERLRRLIREHQGGGHNFGVVSNPEFLREGAAIN
                     DFMRPDRVVIGSRDEEAIAIMRDLYRPLYLIDTPVVITSLEAAELTKYAANAFLATKI
                     SFINEIANLCDRIGCDVHDVARAIGMDNRIGRKFLHPGPGYGGSCFPKDTTALLSVAR
                     DYDSESRIVEAVVEVNRRQREVMVEKIERLVGDLAGTKIAVLGLSFKPETDDMRDAPA
                     IDIVRALVHRGARVRAYDPVAIEQAKKFLPDIEYAEDEYGAVEGADALVFMTEWNQFR
                     ALDLARVRELMRAPKVADLRNIYEPPAMRELGFDYVGVGR"
     gene            2466..3425
                     /locus_tag="AC159P_09460"
     CDS             2466..3425
                     /locus_tag="AC159P_09460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR3695719.1"
                     /translation="MNLSGIVLVTGGAGFVGSHIAAGLAGRGARVRVIDDLSTGHLEN
                     IEEIGGGVDFVRGSLNDADDLRRALEGVELVFHEAAIPSVPRSVERPLESHRACAEAT
                     IALLDAARAAGVRRLVYAASSSAYGDQPTLPKQEEMAPQPLSPYAAAKLAGEFYCQVW
                     ARTYGFETVALRYFNVFGPRQDPSSQYSGVISRFISALMTGARPVIYGDGEQSRDFTF
                     VSNVVDANLRAAEAAGVAGQVVNVANGEKTTLNQLLDTLKQITGKTDVEAEYRDPRVG
                     DVKHSLADITRARELLGYEPKVGLEEGLRETIDWWKQSRFAKI"
     gene            complement(3586..4635)
                     /gene="mtnA"
                     /locus_tag="AC159P_09465"
     CDS             complement(3586..4635)
                     /gene="mtnA"
                     /locus_tag="AC159P_09465"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0033353 - L-methionine cycle [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HIR3695720.1"
                     /translation="MIKTVEWSDEGVRMIDQRLLPTEEKYLTLRSCEEVAEAIKAMVV
                     RGAPAIGISAAMGIALGTMQAVGTNVEDLEQDFDHFCEVMSATRPTAVNLFWAIERMR
                     DRFRRARAAGADAESIKRQLIEEAQTIFKEDIEANRALGRFGGELIPDGATVLTHCNA
                     GALATAGDYGTALGVVRGARDAGKRVAVIADETRPFLQGTRLTAWELAKDEIPVTIIT
                     DNMAGHVMKQGKVDAVVVGADRVAANGDAANKIGTYMVAVLARRHDIPFYVAAPVSTV
                     DLALPTGDQIPIEERDAREVTHVRDQRLAPEGVPVHNPAFDVTPHELIAAIITDKGVA
                     RPPYTESLRKMVEGQ"
BASE COUNT          797 a         1556 c         1560 g          843 t
ORIGIN      
        1 gtggctccca tccatctgca atttaaggga tgttccgcgc ggggttcttg aattctgaga
       61 cgggttggcg agataagtac acacacaaat cgtctcctgc gagttctttc ggagaggact
      121 tgcggcgcgc tctctcaaaa tctttgacag tttcacctac gggaggagat cgtaatgttc
      181 agcagaactt ggagccgtac gcccttgacg cttggcttag ccgccgccgt cttgagtatg
      241 tactcaatgg tggcgttggc cgcgcctcag caggggccga cgggcgacct gtcggtagtc
      301 ggtgaagtca ccgtcaacgg cacgagcgcc atctccggcg cgacagtttt ttcggacagc
      361 accgtcacga cggccaaggg ttcgagcgcc gtcgtcagcc tcggcaaact cggtagggtc
      421 gaagtgttgc cagagtcgag cctcaagctc agcttcaccg acacgggcgt caccgccatg
      481 ctcgaccagg gccgcgtccg cgtctccagc tcttcgggcg tcgccgcgag cgtgacgacc
      541 aaggacggca gcgcggtggc cgacaacagc cagccgaacg tcttcatggt cgatgtcgag
      601 tgcggcaaca cggtcgtctc cacgcagtcg ggccgggtgg agcttcgcgc cggcaactcc
      661 gtgaagcaga tcgcggcggg cagccaggac accgccggcc aggccacccc cggcacgcgc
      721 tgcacccgct tctcgcagcc tgacatgcgc ggcctcggcg gcggcgcgct ggccgcgctg
      781 ctcctcgcgg cgggcggcgc catcgcagcg gccatcttcg cggcttcgag cgacgacgag
      841 ttcaacttcg gcgacggccc catcgtcatc agcccgagcc gttaagttga cgccgacagg
      901 tttcgcttcg cgcgggccat ctccgcgcgc cagttgtggt tgacttcaac acttgcgggt
      961 cacgcgcttc aagctccgcg tgacccgcaa gcttttgcct ggaataatcc cagggttttt
     1021 cgaaaggctt taccaaacgt aatgcacatc gctgtcatcg ggacgggcta cgtcggtctg
     1081 gtcaccggcg cctgttttgc cgagttcggc gtcaacgtta cctgcgtgga cgttgacgaa
     1141 gagaagatca agcaactggc cgaggggaag attccgatct acgagccggg cctcgaccaa
     1201 ctggtcgcgc gcaacgcgca ggccgggcgc ctccacttca ccaccgacat ccgactggcc
     1261 gtcgagcagg cgctcgtcat cttcctcgcc gtcggcacgc cgccgcgcga ggacggctcg
     1321 gctgacctgc gcttcatcga agaggctgcg cgctcggtcg ccgaccacat gaacggctac
     1381 aaggtgatcg tcacgaagtc caccgtcccg gtcggcacgg gcgagcgcct gcgccgcctc
     1441 atccgcgagc atcagggcgg cgggcacaac ttcggcgtcg tctcgaatcc cgagttcttg
     1501 cgcgagggcg ccgcgatcaa cgacttcatg cgccccgacc gcgtcgtcat cggcagccgc
     1561 gacgaggagg ccatcgccat catgcgcgac ctctaccgcc cgctctacct gatcgacacg
     1621 cccgtcgtca tcacctcgct cgaagccgcc gagctgacga agtacgcggc caacgccttc
     1681 ctcgccacca agatttcctt catcaacgag atcgccaacc tctgcgaccg catcggctgc
     1741 gacgtgcacg acgtggcgcg cgccatcggc atggacaacc gcatcggcag gaagtttctc
     1801 caccccggcc ccggctacgg cggctcgtgc ttcccgaagg acacgacggc gctcctctcg
     1861 gtcgcgcgcg actacgactc cgagtcgcgc atcgtcgagg cggtcgtcga ggtcaaccgc
     1921 cgccagcgcg aggtgatggt cgagaagatc gagcggctgg tcggcgacct cgcgggcacg
     1981 aagatcgccg tgctcggcct ctccttcaag ccggagacgg acgacatgcg cgacgcgccc
     2041 gctatcgaca tcgttcgcgc gctcgtccat cgcggcgcgc gcgtgcgcgc ctacgacccc
     2101 gtcgccatcg aacaggcgaa gaaattcctg cccgacatcg agtacgcgga ggacgagtac
     2161 ggagcggtcg agggcgccga cgcgctcgtc ttcatgaccg agtggaacca gttccgcgcg
     2221 ctcgacctcg cgcgcgtccg cgagttgatg cgcgccccga aggtcgcaga cctccgcaac
     2281 atctacgagc cgcccgccat gcgcgagtta ggcttcgact acgtcggcgt cggcagataa
     2341 agggccgtga gccgtgaacc gtcaaccgtg atgagttaaa acagcccccc tctcatcgac
     2401 gttcacggcg cacggtgcac ggcttttcac ctatcacggt ttacggttca cggttcacgg
     2461 cacttatgaa tctttcaggt atcgttctgg tgacgggcgg cgccggtttc gtggggtcgc
     2521 acatcgcggc gggactcgcg gggcgcgggg cgcgcgtgcg cgtgattgac gacctctcga
     2581 cgggccatct tgagaacatc gaagagatcg gcggcggtgt cgatttcgtc cgcggcagct
     2641 tgaatgacgc ggacgacctg cggcgcgcgc tcgaaggcgt cgaactcgtg ttccacgagg
     2701 cggccattcc ctcggtgccg cgctcggtcg agcgcccgct ggaatcgcac cgggcgtgcg
     2761 ccgaggcgac gattgcgctg ctcgacgcgg cgcgggcggc gggcgtgcgc cgcctcgtct
     2821 acgccgcttc gagttcggct tacggcgacc agccgacgct gccgaagcag gaggagatgg
     2881 cgccgcagcc gctctcgccc tacgcggcgg cgaagctcgc gggcgagttt tactgtcagg
     2941 tgtgggcgcg cacctacggc ttcgagacgg tagccttgcg ctacttcaac gtcttcggcc
     3001 cccgccagga ccccagctcg caatactccg gcgtcatctc gcgcttcatc tcggcgctga
     3061 tgacaggcgc gcggcccgtc atctacggcg acggcgaaca gtcgcgcgac ttcaccttcg
     3121 tctccaacgt ggtggacgcc aacctgcgcg cggcggaggc cgcgggcgtc gccggtcagg
     3181 tcgtcaacgt cgccaacggc gagaagacca cgctcaacca actcctcgac acgctcaagc
     3241 agatcaccgg caagaccgac gtggaggccg aataccgcga cccgcgcgtc ggcgacgtga
     3301 aacacagcct cgcagacatc acgcgcgcga gagagttatt gggttacgag ccgaaggtcg
     3361 gattggaaga aggactgcgc gaaactatcg actggtggaa gcagagccgc ttcgcaaaga
     3421 tttaagggcg aagaggggaa aaggaagaca agaagcagcg tgcctctcat caacgaatga
     3481 cgagaggcac gctgttttta gtcatcggct gaggataaga agcacgctgc ttctcatcct
     3541 cccttttcgc ctcttcccct tttcccctct tcgccctcaa gtcttttatt ggccttcgac
     3601 catcttcctc aaactctctg tgtagggcgg gcgggcgacg cctttgtcgg tgatgatggc
     3661 ggcgatgagt tcgtggggcg tcacgtcgaa ggcggggttg tggacgggga cgccttcggg
     3721 ggcgaggcgc tggtcgcgga cgtgcgtgac ctcgcgggcg tcgcgctctt cgatgggaat
     3781 ctggtcgccg gtggggagcg cgaggtctac ggtcgagacg ggcgcggcga cgtagaaggg
     3841 gatgtcgtgg cggcgcgcga ggacggcgac catgtaggtg ccgatcttgt tggcggcgtc
     3901 gccgttggcc gcgacgcggt cggcgccgac gaccaccgcg tccaccttgc cctgcttcat
     3961 cacgtgcccg gccatgttgt cggtgatgat ggtgacggga atctcgtcct tcgccagctc
     4021 ccaggccgtg aggcgcgtgc cttgcaggaa ggggcgggtc tcgtctgcga tgacggcgac
     4081 gcgtttgccc gcatcgcgcg cgccgcggac gacgccgagc gcggtgccgt agtctccggc
     4141 ggtggcgagc gcgcccgcgt tgcagtgcgt cagcacggtc gcgccgtccg gtatcagctc
     4201 gccgccgaag cggcccaaag cgcggttggc ctcgatgtct tctttgaaga tggtctgcgc
     4261 ttcctcgatg agctgccgct tgatggactc ggcgtcggca cccgcggccc tggcgcggcg
     4321 gaagcggtcg cgcatgcgct cgatggccca gaagaggttg acggcagtgg gccgggtcgc
     4381 actcatcact tcgcagaagt ggtcgaagtc ctgttcgagg tcttcgacgt tcgtgccgac
     4441 ggcctgcatc gtgccgagcg cgatgcccat cgcggcggag atgccgatgg cgggcgcgcc
     4501 gcgcacgacc atcgccttga tggcctcggc cacctcttcg caggagcgta aggtgaggta
     4561 cttctcttcg gtcggcagaa ggcgctggtc gatcatgcgg actccctcgt ccgaccactc
     4621 gacggtctta atcattggcg gtatgaatct cctcggatgt tttgcataag tgaaggcccg
     4681 cgatgctgag atgccaccac gtcggccgta aaaaagaagg gctatcttaa gggagcgagg
     4741 cggggcgggc aacctt
//
LOCUS       DBNGOH010000478         3373 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_803849, whole genome shotgun sequence.
ACCESSION   DBNGOH010000478 DBNGOH010000000
VERSION     DBNGOH010000478.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3373)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3373)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3373
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_803849"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..799
                     /locus_tag="AC159P_09470"
     CDS             <1..799
                     /locus_tag="AC159P_09470"
                     /inference="COORDINATES: protein motif:HMM:NF013740.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HIR3695721.1"
                     /translation="EIADRISASLSRYLGVRFEETRASLRDAFAEAVVRSGRYFQEVR
                     ERAAELLAAATVFFFTVYYVLTRREHIVPYIKLRIPGEFLPLYERLTENVGGALRGAL
                     WAVFIDQIVKALVILVCNFVFDVPLAVALALAAFLVGFFPLLGVWVVYVPVSVYLLVF
                     RDDPTAAGIYFVVGTLITLASTTFLRPMLASREAKAFNFFWMLVALIAGVLVFGIPGI
                     VLGPAILGFAKAVLDTLVGDVRYETSLLKEEREQQAGAETATPESGV"
     gene            910..1818
                     /locus_tag="AC159P_09475"
     CDS             910..1818
                     /locus_tag="AC159P_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011024783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HIR3695722.1"
                     /translation="MNRELFKVSEEVEAALGARRAVVALESTVIAHGLPRPSNLETAR
                     RLEEAVRAGGATPATVAIIGGEIHVGLSGEQLERFANTDGVVKVSRRDLAVVAARGLD
                     GATTVATTAWAAHRAGLKVFATGGVGGVHRGDHGDVSADLPELARTPIVVVCSGAKSV
                     LDLATTREWLETHGVTVVGYGCDELPAFYTRRSGLAVDARAERAAEVARLARARDRLG
                     LEGALLVAVPVPAEAEVDAGLMEEVLSRALADAERDGVRGRALTPFLLARMGEASGGA
                     TLRANVALLENNARVAAEIAVALESC"
     gene            2181..>3373
                     /locus_tag="AC159P_09480"
     CDS             2181..>3373
                     /locus_tag="AC159P_09480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695723.1"
                     /translation="MRMRAQLSDGGGANVLRFDPPVRKAEAELPEPIRLIVSNLGVGV
                     EADLIRGTFASLMELLADLQNVEAIGSQAAHVEETVAAFASVHDKTLDLVAHIEQQVL
                     LVEDISKSPGSELLDVAFALRHELRRAFDLDIAELDGGRPFESAEGRVAHAHGVLRNC
                     FRQCIVMLAQAFEPALSERTVFADAASRLEESILLRDALSALLDSAQEAVKKSFPQSA
                     VSVIEQLDVFRRDGMPFLMRRDWEMFDTFENEIVAAKDAGEFDYAAKKLLVGLETLHG
                     QVRMRSVFDDERKAAPAPKRFDFKLARGLAAVRPATWSAVAASLLLACGLAFFVDTTL
                     VTRGSQLAADAAQPTVNATSEQAAPVTKEPAKPAAPAKAEQPAGEKQSAGGLTLQVGA
                     FRDGPA"
BASE COUNT          517 a         1140 c         1168 g          548 t
ORIGIN      
        1 cgagatcgcc gaccgcatca gcgcctcgct ctcgcgctac ctcggcgtca ggttcgagga
       61 gacgcgcgcc tctctgcgcg acgccttcgc cgaggcggtc gtgcgctcgg gccgctactt
      121 tcaggaggtg cgcgagcgcg cggcggagct gctcgccgcg gccacggtct tcttcttcac
      181 cgtctactac gtgctcacgc ggcgcgagca catcgtcccc tacatcaagc tgcgcatacc
      241 gggcgagttt ttgccgctct acgagcggct gaccgagaac gtcggcgggg ctttgcgcgg
      301 ggcgctgtgg gccgtcttca tcgaccagat cgtcaaggcg ctcgtcatcc tcgtctgcaa
      361 cttcgtcttc gacgtgccgc tcgcggtggc gctggcgctc gcggcgttcc tcgtcggctt
      421 cttcccgctg ctcggcgtgt gggtggtcta cgtgccggtc agcgtctacc tgctcgtctt
      481 ccgcgacgac ccgacggcgg cgggcatcta cttcgtcgtc ggcacgctca tcaccctcgc
      541 ctcgaccacg ttcctccggc ccatgctggc ctcgcgcgag gcgaaggcgt tcaacttctt
      601 ctggatgctg gtggcgctca tcgcgggcgt gctcgtcttc ggcatccccg gcatcgtcct
      661 cggccccgcc atcctcggct tcgccaaggc cgtcctcgac acgctcgtcg gcgacgtgcg
      721 ctacgagact tcgctcctca aagaggaacg cgaacagcag gccggagccg agacggcaac
      781 cccggagtcc ggagtctgaa gtctggagtc cggagtccga accatcgagc ataaacgacg
      841 acgaacaagc accggctcag tcataatcaa caagacattt tcgttctgac tccagactcc
      901 ggacttctta tgaatcgaga actcttcaag gtcagcgagg aggtggaagc ggcgctcggc
      961 gcgcgtcgcg ccgtggtggc gctcgaatcg acggtgatcg cgcacgggct gccgcgccct
     1021 tcgaatctgg agacggcgcg gcgcctggaa gaggccgtgc gcgcgggcgg ggcgacgccc
     1081 gcgaccgtgg cgatcatcgg cggcgaaatc cacgtcgggc tgagcggcga gcagctcgaa
     1141 cgcttcgcca acaccgacgg cgtggtgaag gtgagccgcc gcgatctggc cgtggtcgcg
     1201 gcgcgcggcc tcgacggcgc gacgaccgtg gcgacgaccg cgtgggccgc gcaccgcgcg
     1261 gggctgaagg tcttcgcgac gggcggtgtc ggcggcgtcc accgcggcga ccacggggac
     1321 gtgtcggcgg acttgccgga gctggcgcgc acgccgattg tcgtcgtctg ctcgggcgcg
     1381 aagtccgtcc tcgacctcgc gacgacgcgc gagtggctgg agacgcacgg ggtgacggtg
     1441 gtcggctacg gctgcgacga gctgcccgcg ttctacacgc ggcggagcgg cctcgcggtg
     1501 gacgcgcgcg ccgagcgggc ggcggaagtg gcccgcctcg cgcgggcgcg cgaccggctc
     1561 ggcctcgaag gcgcgctgct cgtcgcggtg cccgtccccg cggaggcgga ggtcgatgca
     1621 ggcttgatgg aagaagtgct gtcgcgcgcg ctggcggacg ccgaacggga cggcgtgcgc
     1681 gggcgcgcgc tgacgccctt cctgctcgcg cgcatgggcg aggcgagcgg cggcgcgacc
     1741 ctccgcgcca acgtcgcgct gcttgagaac aacgcgcgcg tcgccgcgga gatcgccgtc
     1801 gcgctggaaa gctgttagct gttagttact gaccgacggt tgatgaccgc gcctctccca
     1861 tcaagcgtca tccgttaagc taatagctaa cagccaacag ctaacagcca catgctgacc
     1921 gccttcaatc aacttgaatc gaggggcggg caatctgacg aaatccgtca gccgcggcga
     1981 gcggctttca ggaaaaccgc ctgtttcccc tgaaaaagca cgcctcacat ggaaaaaagc
     2041 gtggcacgtg aatggctaag taaggcgtgt ccaccgcggt cgggcgtttc ccgccgccgc
     2101 cgctttcgaa gcttgtccct tgaacccgaa cccacttctc acgttctctg attacttccc
     2161 aacgaaccga atggtgattg atgcgtatgc gtgcacaact gtctgacgga gggggcgcga
     2221 acgtgctgcg tttcgacccg cccgtgcgga aggcggaggc cgagttgccc gagcctatcc
     2281 gcctcatcgt gagcaacctc ggcgtgggcg tggaggcgga cctgatccgc ggcacgttcg
     2341 cgtcgctgat ggaactcctc gccgatctgc aaaacgtcga agccatcggt tcgcaggcgg
     2401 cccacgtcga ggaaacggtc gcggccttcg cgtcggtgca cgacaagacg ctcgacctcg
     2461 tcgcgcacat cgagcagcag gtgttgctcg tcgaagacat ctcgaagagt ccggggagcg
     2521 aactgctcga cgtggccttc gcgctcaggc acgagttgcg ccgcgccttc gacctcgaca
     2581 tcgcggagct ggacggcggt cgcccgttcg agtcggcgga ggggcgtgtg gcccacgcgc
     2641 acggcgtctt gaggaactgc ttccggcagt gcatcgtgat gctggcgcag gcgttcgagc
     2701 cggcgctcag cgagcgcacc gtgttcgccg acgcggcctc gcgcctggaa gagtcgatcc
     2761 tgctgcgcga cgcgctgtcg gcgctgctcg actctgcgca ggaggcggtg aagaagagct
     2821 tcccgcagtc ggcggtctcg gtcatcgaac agctcgacgt gttccgccgc gacggcatgc
     2881 ccttcctcat gcgccgcgac tgggagatgt tcgacacctt cgagaacgag atcgtcgcgg
     2941 cgaaggacgc gggcgagttc gactacgcgg cgaagaagct gctggtcggc ctcgaaacgc
     3001 tgcacgggca ggtgcgcatg cgctcggtct tcgacgacga acgaaaggcc gcgcccgcgc
     3061 ccaaacgctt cgacttcaag ctcgcgcgcg gactcgctgc cgtgcgcccc gcgacgtggt
     3121 cggcggtggc ggcctcgctg ctcctggcgt gcggcctcgc cttcttcgtc gatacgaccc
     3181 tcgtcacgcg cggcagccag ctcgcggcgg acgcggcgca gccgaccgtc aacgcgacga
     3241 gcgagcaggc cgcgccggtg acgaaagaac cggcgaagcc cgcggcgccc gcgaaggcgg
     3301 agcagccggc gggggagaag cagtcggcgg gcggcctgac gttgcaggtc ggcgccttcc
     3361 gcgacggccc ggc
//
LOCUS       DBNGOH010000479         1988 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2087142, whole genome shotgun sequence.
ACCESSION   DBNGOH010000479 DBNGOH010000000
VERSION     DBNGOH010000479.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1988)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1988)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1988
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2087142"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..151)
                     /locus_tag="AC159P_09485"
     CDS             complement(<1..151)
                     /locus_tag="AC159P_09485"
                     /inference="COORDINATES: protein motif:HMM:NF013545.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HIR3695724.1"
                     /translation="MAEREEITVRAERRSGRGKNDARRLRVRGMVPITLYGGEGEAVS
                     AAAPLK"
     gene            complement(197..1162)
                     /locus_tag="AC159P_09490"
     CDS             complement(197..1162)
                     /locus_tag="AC159P_09490"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005007376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HIR3695725.1"
                     /translation="MSTTGGIKVFSGNANRALTEEICNYLSCDPGSATTSRFSDGEFN
                     FQITENVRGGDVFIVQPTCPPTDSNLMELLVMLDAFRRSSAERVTAVIPYYGYARSDK
                     KDRPRVPIGAKLVANLIATAGADRVLTMDLHAAQIQGFFDIPVDHLFAAPVFIGYYQA
                     HPLPNLTVVAPDTGGAERARAYAKRLTSVDGSPAQLALCDKRREKANVAEVMNIVGDV
                     RGRNCLIVDDMCDTGGSLTKVAAALRSAGADRIHACFTHPVLSGRACGKLEGSDIERV
                     VTTNTIPLGDDACESEKIKVLSIAPLLAKAIKSIHEETSVSSLFV"
     gene            complement(1259..1330)
                     /locus_tag="AC159P_09495"
     tRNA            complement(1259..1330)
                     /locus_tag="AC159P_09495"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1296..1298),aa:Gln,seq:ttg)
     gene            complement(1361..>1988)
                     /gene="ispE"
                     /locus_tag="AC159P_09500"
     CDS             complement(1361..>1988)
                     /gene="ispE"
                     /locus_tag="AC159P_09500"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HIR3695726.1"
                     /translation="GGGLGGGSSNAAVALLGLSHLWDSKAGKRELVELGARLGADVPF
                     FFTGGTALGTGTGTDIAPLADVPRRHLIVVTPAVKVSTAEAYKALNAPALTKAEGAVM
                     LPISHAEADFSDSHRAVMRNDFEAVVIRLHPEIGRVKDALLGAGARRAMLSGSGSSVF
                     GVFDSEGEAELALGALDAEAGWQIFPCATLARGEYRAALGACAGVLEA"
BASE COUNT          341 a          695 c          611 g          341 t
ORIGIN      
        1 ccttcagcgg cgccgccgcc gagacggcct cgccctcgcc gccgtagagc gttatgggaa
       61 ccatgccccg gacgcgcagg cgccgcgcgt cgttcttgcc gcgcccgctg cgccgctcgg
      121 cgcgaactgt gatttcttct ctttcagcca ttgtcaaaac ctctctggtc aaaccgtcag
      181 tttttgcccg ccgtcgttag acgaagagcg aagagacact cgtctcctcg tggatcgact
      241 tgatcgcttt cgccagaagc ggcgcgatgc tcagaacctt tatcttctcc gactcgcacg
      301 cgtcgtcgcc gagcgggatc gtgttcgtcg tcacgacgcg ctcgatgtcc gagccttcca
      361 gcttcccgca cgccctgccc gacagcacgg ggtgcgtgaa gcacgcgtgg atgcggtcgg
      421 cccccgcgct cctcaaggcc gccgccacct tcgtcaaaga gccgcccgtg tcgcacatgt
      481 catcgacgat caggcagttg cgcccgcgca cgtcgccgac gatgttcatc acctccgcga
      541 cgttcgcctt ctcgcgccgc ttgtcgcaca aagcgagctg cgccgggctg ccgtccacgg
      601 acgtgagccg cttggcgtaa gcgcgcgcgc gctcggcgcc acccgtgtcc ggcgcgacca
      661 ccgtcaggtt cggcaaaggg tgcgcctggt agtagccgat gaagaccggc gccgcgaaca
      721 ggtgatcgac cgggatgtcg aagaagccct gaatctgcgc cgcgtgcaag tccatcgtca
      781 gcacgcggtc ggcgccggcg gtcgcgatca ggttcgccac cagcttcgca ccgatgggca
      841 cgcgcgggcg gtctttcttg tccgagcgcg cgtaaccgta ataagggatc accgccgtca
      901 cgcgctcggc ggaagagcga cggaacgcgt cgagcatgac gagcagttcc atcaggttcg
      961 agtccgtcgg cgggcacgtc ggctgcacga tgaacacgtc gccgccgcgc acgttctcgg
     1021 tgatctggaa attgaactcg ccgtcggaga agcgcgacgt ggtcgcgctg ccggggtcgc
     1081 agctcaggta gttgcaaatc tcctcggtca gcgcgcggtt cgcgttaccc gaaaagactt
     1141 tgatgccgcc ggtcgtgctc atagaaaggc agtggcgagt gacgagcggc aagtgacgag
     1201 tccgagacag gttttcaact tgtcacccgt cacttgccgt tcgtcgctgc tgtttaagct
     1261 ggggcggaag gattcgaacc tacgaatgcc ggttccaaag accggtgcct taccacttgg
     1321 cgacgcccca atccttttcg cgccttttcg tgttagagcc tcaggcttcc agcaccccgg
     1381 cgcacgcgcc caacgccgcg cggtactcgc cgcgcgcgag cgtggcgcac gggaagattt
     1441 gccaccccgc ctcggcgtcc agcgcgccga gagcaagctc cgcttcgccc tcgctgtcaa
     1501 agactccgaa cacgctcgaa ccgctgcccg aaagcatcgc acggcgcgcg cccgctccga
     1561 ggagcgcatc tttcacgcgc ccgatttcgg gatgcagtcg gatgaccacg gcctcgaaat
     1621 cgttgcgcat caccgcacgg tgagaatccg aaaaatcggc ctccgcgtgc gaaataggca
     1681 gcataacggc gccctccgcc tttgtcaagg caggggcatt cagggccttg tacgcctcag
     1741 ccgtagagac tttgaccgcg ggcgtgacga cgatcaagtg ccgccgcggc acgtccgcga
     1801 gcggggcgat gtccgtcccc gtgcccgtcc ccaaagcggt cccgcccgtg aaaaagaagg
     1861 gcacgtccgc gccgagacgc gcgcccaact cgaccagctc gcgcttccct gctttcgaat
     1921 cccacaggtg agaaagtccc agcaaagcga ccgccgcgtt cgacgagccg ccgccgagtc
     1981 cgccgccg
//
LOCUS       DBNGOH010000480         2447 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4470447, whole genome shotgun sequence.
ACCESSION   DBNGOH010000480 DBNGOH010000000
VERSION     DBNGOH010000480.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2447)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2447)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2447
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4470447"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1173)
                     /locus_tag="AC159P_09505"
     CDS             complement(<1..1173)
                     /locus_tag="AC159P_09505"
                     /inference="COORDINATES: protein motif:HMM:TIGR02608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="delta-60 repeat domain-containing protein"
                     /protein_id="HIR3695727.1"
                     /translation="MPPSTKSAAQFTALCLLLSSLLTGVVPTARAQQIQVSAANPPSA
                     EQGTVSLDVRVTGKGFKNGARAKFQVTGTNDPGGVQVNSTTFVSSTEVVANVTVAETA
                     VVANFDIQVQNTDGRTGKGTELFAVLAKGGGQAACPAPQPAPTGDTRCFAALPGCLDT
                     TFGSSGLVVTDTNGPDIDHADAVLVQGDGKIVAAGHTDSSASATIAIDFLLVRYNADG
                     SLDTSFGDPDPLNPPLRLGYVITPLTTGYDRPRAALLQPDGKIVAGGWNNLTASMLVV
                     RYNTDGTLDPTFGSDGKVFVDFGSSSSVWGMAIQSDGKLLLSGDAGQRFAAARLNPNG
                     ALDTSFATGGKLVVSASSKRRGRSYATAVAVQRIPAVTGEERIILGGWSDSDSSYVF"
     gene            complement(1276..>2447)
                     /locus_tag="AC159P_09510"
     CDS             complement(1276..>2447)
                     /locus_tag="AC159P_09510"
                     /inference="COORDINATES: protein motif:HMM:NF024180.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HIR3695728.1"
                     /translation="SPEQKRQALDLFSRELAEAERSYQNFLDDLPNTEPGSATVRALK
                     VPTVAEVQRRLDARTALVMYVMAEEALHVFVVTAESLRARTVPVRAADLKVKVDLLRG
                     VMLRHHTDEWRPPAARLYELLVGPVKEAGWLRGVERLYVVPHANLHYVPFAALRPAGV
                     SARFLVDEYDLAYLPAAAALVFGDQPPRPSESVLAVAPSSTRLRYTQQESRGVAELFP
                     KRSALLLGARATESSFKRMAGDYDVIHLATHGYFNKLNPLLSGLAFEPDAEEDGRLEA
                     HEILGLRLRARLVTLSACDTALGGGYFADVPAGDDLVGLTRSFLFAGSPTVLASLWEV
                     NDLSAVRLMRGFYRQLGRGDKAAALAAAQRERRRRGGRYAHPYYWGAFVLVGQMN"
BASE COUNT          395 a          808 c          848 g          396 t
ORIGIN      
        1 aaagacataa gacgaatcgc tgtcagacca gccgccgagg atgattcttt cctcacccgt
       61 cacggcagga attctctgga cggcgacggc ggtggcgtag gagcggccac gccgcttgct
      121 gctggcacta acgacgagtt tgcccccggt ggcgaagctg gtgtcgagcg ccccgttagg
      181 attgagtcga gccgcggcga agcgctggcc ggcgtccccg ctcagaagca gcttgccgtc
      241 cgactggatc gccattcccc acacggagga ggagctgccg aaatcaacga acaccttgcc
      301 gtcgctgccg aaggtcgggt cgagcgtgcc gtctgtgttg tagcggacga cgagcatcga
      361 agcggtgagg ttgttccacc cgcctgcgac tatcttgccg tccggctgaa gaagggctgc
      421 gcgcgggcgg tcgtagccgg tggtgagcgg cgtgatgacg tagccgaggc ggagcggcgg
      481 gttgagcggg tcggggtcgc cgaatgaggt gtcgaggctg ccgtcggcgt tgtaacggac
      541 caacagaaag tcaatcgcga tcgtagcgct cgccgaactg tccgtgtgtc cggccgcgac
      601 gatcttgccg tcgccctgga cgagcacggc atccgcgtgg tctatgtcgg ggccgttggt
      661 gtcagtaacc accagcccgc tgctgccgaa agtcgtgtcc aagcatcccg gcagcgcggc
      721 gaaacacctc gtgtcgcccg tcggcgcggg ttgaggtgcc gggcaggcgg cctgcccccc
      781 gcccttggcg aggaccgcga acagttcggt gcctttgccc gtgcgcccat cggtgttctg
      841 cacctgaatg tcgaagttgg cgacgacggc ggtctccgcg accgtgacgt tggcgacgac
      901 ttcggtcgag ctgacgaagg tcgtcgagtt gacctgcacc ccgccggggt cgttggttcc
      961 ggtgacctgg aacttcgccc gcgccccgtt cttgaagccc ttgccggtga ctctgacgtc
     1021 gaggctgacc gtgccctgct cggcggacgg agggttcgcg gcgctgacct gaatctgctg
     1081 cgcccgtgcg gtcgggacga cgccggtgag tagagatgac aacaacagac agagggcggt
     1141 gaactgtgcg gctgactttg ttgagggggg catgtcaagt ctcctaacat cggttgggcg
     1201 ccatccgcag gggcattgga aaagtcgtcc cgttgggtct acacggacac gccgacatct
     1261 tttccgcggc ggccttcagt tcatctgtcc gaccagcacg aacgcgcccc agtagtaggg
     1321 gtgtgcgtag cgcccgccgc gccggcgccg ctcgcgctgg gccgcggcga gcgccgccgc
     1381 cttgtcgccc cggcccagtt ggcggtagaa gccccgcatc aggcgcaccg ccgaaagatc
     1441 gttgacctcc cacaagctcg ccagcaccgt ggggctgccg gcgaacagaa acgagcgcgt
     1501 cagcccgacc agatcgtccc cggcgggcac gtccgcgaag tagccgccgc caagcgccgt
     1561 gtcgcaggcc gagagcgtga ccagccgcgc cctcagacgg aggccgagaa tctcgtgggc
     1621 ttcgagccgc ccgtcctcct cggcgtccgg ctcgaaggcg aggcctgaca gcagcgggtt
     1681 gagcttgttg aagtagccgt gggtcgccag gtggatcacg tcgtagtcgc ccgccatgcg
     1741 cttgaacgag ctttccgtgg cgcgcgcgcc caaaagcagc gcgctccgtt tcgggaacaa
     1801 ctcggccacg ccccgcgact cctgctgcgt gtaccgcagc cgcgtgctcg acggcgccac
     1861 ggccagcacc gactcgctcg ggcgcggcgg ctggtcgccg aagacgagcg ccgccgccgc
     1921 cggcaggtaa gccagatcgt attcgtccac cagaaaccgg gcactgacgc cagcagggcg
     1981 gagcgccgcg aaggggacgt aatgaaggtt ggcgtgcggg acgacgtaga ggcgttcgac
     2041 gccgcgcagc cagccggcct ctttaaccgg cccgaccagt agctcgtaca gtctggcggc
     2101 ggggggccgc cactcgtccg tgtggtggcg gagcatgacg ccgcgcaaca ggtccacctt
     2161 gacctttagg tcagccgccc ggacggggac agtccgcgcg cgcaagctct ccgccgtgac
     2221 gacaaaaacg tgcagcgcct cctcggccat cacgtacatg accagcgccg tgcgggcgtc
     2281 cagccgccgc tgcacctctg cgaccgtcgg caccttgaga gcgcgcaccg tcgccgatcc
     2341 cggctccgtg tttggcaggt cgtcgagaaa gttctggtag ctgcgctcgg cctcagccag
     2401 ctcacgtgag aagaggtcga gcgcctgccg cttctgctcg ggggaag
//
LOCUS       DBNGOH010000481         3037 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_989116, whole genome shotgun sequence.
ACCESSION   DBNGOH010000481 DBNGOH010000000
VERSION     DBNGOH010000481.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3037)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3037)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3037
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_989116"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..455
                     /locus_tag="AC159P_09515"
     CDS             <1..455
                     /locus_tag="AC159P_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013617419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="galactosylceramidase"
                     /protein_id="HIR3695729.1"
                     /translation="GFIPKGYFLELGADGGLRLVVVRGKKDKKALVGDAEQQALIKAG
                     KDEGEGGEKLLATARLPNVGPGRWHNLKLRFEGSAITGLVDGTTVLSATDALYERGMA
                     GLLAGGEKAKLSTPYFDNLLIKGVNAAVPKPSRAAPGQSPIYKSSAKR"
     gene            729..2033
                     /locus_tag="AC159P_09520"
     CDS             729..2033
                     /locus_tag="AC159P_09520"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011109141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HIR3695730.1"
                     /translation="MPPIRVPVFPKRDFVITAFGAKPGGEADNTAAIRQAIAACNKAG
                     GGRVVVPAGLWLTGAVHLKSNVNLHLSEGAVLSFSDDPKDYLPAVRSSWEGWECFNYS
                     PLIYAFEAENVAITGRGRIEPRMGTWKKWFARPPAHMEALKRLYTMGSTGVSVEQRRM
                     AEGENNLRPQLIQFNRSRNVLVEDIKIRNSPFWTIHLLLCESVVVRRVDVSAHGHNND
                     GVNPEGTRNLLVEDSRFDQGDDAVAIKSGTNHDGWRLGRPSENIVIRNSIVIRGHQLV
                     AIGSELSGGVRNVYVHDCRFDAATEPFNLLYIKTNRRRGGFVENVTMERVEATGTKFG
                     VFGIETDVLYQWRTLVPTYEERLTSIRGITVRDVKVGRTATPFRILGDERAPVRDVLL
                     DNITIGTVRGQKNRYENARNVKETRIRIGTFIEEPDQENKNR"
     gene            complement(2075..2419)
                     /locus_tag="AC159P_09525"
     CDS             complement(2075..2419)
                     /locus_tag="AC159P_09525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0032196 - transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HIR3695731.1"
                     /translation="MVGQPVECGLAARRGVLPLALPRGSQLFMDGGYTDYAAAITDGV
                     EFSPSRKSNSERGDDVWRGYYKQLMRKRIETVFGRIASLFPKHIHAVTLDGFLLKVSM
                     FVIAFALDKAFI"
     gene            2565..>3037
                     /locus_tag="AC159P_09530"
     CDS             2565..>3037
                     /locus_tag="AC159P_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HIR3695732.1"
                     /translation="MEDLLRDVARRAIRYHAGLNARAVAPSSEAVNNLARLREPFPQQ
                     PADPETVLALLDEVGSPATVSSAGGRFFGFVTGGSLPAALAANWLAGAWDQNAFSFAS
                     SPAAVVFEEVALGWLLDALALPPECGGAFVTGATMANFTALAAARNAVLSGRGWDV"
BASE COUNT          565 a         1013 c          982 g          477 t
ORIGIN      
        1 acggcttcat cccgaaaggc tacttcctcg aactcggcgc cgacggcggg cttcgtctgg
       61 tcgtcgtccg cggcaagaag gacaagaagg cgctggtggg cgacgccgaa cagcaggcgc
      121 tcatcaaggc cgggaaggac gagggcgagg gcggggagaa gttgctcgcc accgccaggc
      181 tcccgaacgt cggccccggc cgttggcata acctgaagct ccgcttcgaa ggctcggcca
      241 tcacgggact cgttgacggg acgacagtcc tgagcgcgac cgacgcgctc tacgagcggg
      301 gcatggccgg gctgctggcg ggcggcgaga aggcgaagct cagcacgccg tatttcgaca
      361 acttgctaat caaaggcgtc aacgccgccg tgcccaagcc ttcacgagcc gcgccgggcc
      421 agtcaccgat ctacaagtcg tcggcaaagc gctgatgagt cgttccgcta atcacttgat
      481 gagcacaccg acaccaatac ctatcatggg aagcagaata aagggtcgat caccggtgcc
      541 gtctccgaac ggacttcgga gacgaggggc tacaaaaacg gggggcgttt tcgggagcct
      601 cacccccgct gcccccctcg ccacggcttt gcgggcctgc ctcgccgtgc tgctgctgct
      661 cgccgccgcc ccccgcccaa gccggacgcg tccgtcacga gaaaatctcc gtcaaggctc
      721 ctttccccat gccgcccatc cgtgtccccg tcttccccaa acgggatttc gtcatcaccg
      781 ctttcggcgc caagccgggc ggcgaggccg acaacaccgc ggccatccgc caggccatcg
      841 ccgcgtgtaa taaggccggc ggaggccgcg tcgtcgtccc cgcagggctt tggctcaccg
      901 gcgccgtcca cctcaaatcg aacgtcaacc tccacctctc cgagggcgcc gtcctctcct
      961 tcagcgacga cccgaaggac tacctgcccg cggtgcgttc gagctgggag ggctgggagt
     1021 gcttcaacta ctcgccgctc atctacgcct tcgaggccga gaacgtcgcc atcaccggcc
     1081 ggggccggat cgagccgcgg atgggcacct ggaagaagtg gttcgcgcgc ccgcccgcac
     1141 acatggaggc gctcaaaagg ctctacacga tgggttcgac aggcgtgtcg gtcgagcagc
     1201 gccggatggc cgagggcgag aacaatctgc ggccccagct catccagttc aaccgcagcc
     1261 gcaacgtcct cgtcgaggat ataaaaattc gtaacagccc gttctggacg atccacctgc
     1321 tgctgtgcga gtcggtggtc gtccgccgcg tggatgtctc ggcccacggg cacaacaacg
     1381 acggggtcaa cccggagggg acgcgcaacc tgctggtcga agacagccgg ttcgaccagg
     1441 gcgacgacgc cgtcgccatc aagagtggga cgaaccacga cgggtggcgg ctcggcaggc
     1501 cgtcggagaa catcgtcatc cgcaacagta tcgtcatccg cggccaccag ctggtcgcca
     1561 tcgggagcga gctgtcggga ggcgtccgca acgtctacgt gcacgactgc cggttcgacg
     1621 ccgcgaccga gccgttcaac ctgctctaca tcaagaccaa ccggcggcgg ggcggcttcg
     1681 tcgagaacgt gacgatggag agggtcgagg cgacggggac gaagttcggc gtgttcggga
     1741 tcgagaccga tgtcctttac cagtggcgga cgctggtgcc gacctacgag gagcggctga
     1801 ccagcatccg cggcatcacg gtgcgcgacg tgaaggtggg caggacggcg acgccgttcc
     1861 gcatcctcgg cgacgagcgc gcgccggtgc gggatgtcct gctcgacaac atcacgatcg
     1921 gcacggtgcg cgggcagaag aaccgctacg agaacgcgag gaacgtcaag gagactagaa
     1981 tccgcatcgg cacgttcatc gaggagccgg atcaggagaa caagaacaga tagccgccgc
     2041 cgactttctc gaccaactac atgaacccaa gttgctagat gaacgccttg tccaaagcaa
     2101 aggcgatgac gaacatcgag accttcaaca agaacccgtc gagcgtaacg gcatgaatgt
     2161 gcttcgggaa caagctggcg atccggccaa acacggtctc gatgcgcttg cgcatcagtt
     2221 gcttgtaata gccgcgccaa acatcatctc cacgctccga gttactcttg cgcgacggcg
     2281 agaactcgac tccgtcagtt attgccgccg cgtagtcggt gtagccgcca tccatgaaga
     2341 gttgactgcc ccgcggcaac gcgagcggca gcacgccgcg ccgcgccgcc aggccgcact
     2401 cgacgggctg cccgaccacc atatcaccat caccgagaat gatcgcccgg ttcgctgccc
     2461 ggaagaccta agcgcctgcc gcgtgaatgt tatgattccg cttccgcggg atacggcatt
     2521 cgtggtgggg atgtggcggc aggttcgata gtgggagggg ttggatggaa gacctgttac
     2581 gagacgtcgc gcgacgcgcg attcgttacc acgccggtct gaacgcgcgc gccgtcgccc
     2641 cgtcgtccga ggcggtcaac aatctggcgc ggctcaggga gccgttcccg caacagcccg
     2701 cggaccccga gaccgttctc gccctgctcg atgaggtcgg ctcgcctgcc acggtgtctt
     2761 ccgcgggcgg acgattcttc gggttcgtga cgggcggctc gctgcctgcc gcgctcgccg
     2821 ccaactggct cgccggggcg tgggatcaga acgccttcag cttcgccagc tcgcccgcgg
     2881 cagtcgtctt cgaggaggtc gccctcgggt ggctcctcga cgcgttggcg ctaccgccgg
     2941 agtgcggcgg cgctttcgtg accggcgcga ccatggccaa cttcaccgcg ctggccgccg
     3001 cccgcaacgc cgtgctgagc gggcgcgggt gggatgt
//
LOCUS       DBNGOH010000482         4593 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2087247, whole genome shotgun sequence.
ACCESSION   DBNGOH010000482 DBNGOH010000000
VERSION     DBNGOH010000482.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4593)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4593)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4593
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2087247"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..895)
                     /locus_tag="AC159P_09535"
     CDS             complement(<1..895)
                     /locus_tag="AC159P_09535"
                     /inference="COORDINATES: protein motif:HMM:NF023169.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMIN domain-containing protein"
                     /protein_id="HIR3695733.1"
                     /translation="MNDIPRRRARRSLLFVALLLVAAPALRAQTAEQFASAQPRLITA
                     NTTTAKPLRLKRIAPLKVSETPEGTRVTVASDAPLDDYAARREGDRFVVTLPHAELAQ
                     GPSELQGGGFERARAERHGDGLRLSFRLRDGVEASFAQSFNRLDIFFKTRRQQPASAV
                     GGNVPADASAPAVSKDNATAKKSSDDSKTEASKAPTEGGLTAAEREAMRAMLRRVEEL
                     EARVKELEAERAKAVAASAATSTTSVASHTASAGASPTAVDESAAVPAPVNDVAGAGA
                     GGHGSQDAHGQHEEAAPAGPPR"
     gene            1430..2287
                     /locus_tag="AC159P_09540"
     CDS             1430..2287
                     /locus_tag="AC159P_09540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695734.1"
                     /translation="MGEAIRNATVVAGIVGFIVGLAVSWVSISLWLNGLHWGVVPLSA
                     LAGMCCGAWVGRRIAIYSRREAARLILEGNRIVLEDIGRQTARFVALFHDKDEEDASA
                     LDAFLAELRPGETASGGQDLLRQAFTQYYTARYAGDLKRRHEAAYFANCLAVLHEHIR
                     LEPYIKKSMPYIIRKCATKRLMQFDVGAVSLTVSEEVPRLDGVPFPDSLHDLSSRELS
                     DFLGGAQGWGGDLVKSPARDWTNLRERMRYIVQLFRVMHLEPSVFAEPYTPTQGERPL
                     PFAAELPRR"
     gene            complement(2360..4498)
                     /locus_tag="AC159P_09545"
     CDS             complement(2360..4498)
                     /locus_tag="AC159P_09545"
                     /inference="COORDINATES: protein motif:HMM:NF025869.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA-like domain-containing protein"
                     /protein_id="HIR3695735.1"
                     /translation="MSQSSFYVTGGTLKRDAPSYVARRADHDLYEGLSEGKFCYVLTS
                     RQMGKSSLMVSTAARLRSEGVTVAVIDLTAIGQNLSAEQWYDGLLNRVGRQLDLEDEL
                     EDFWDEHERVGPLQRWTRAVREVVLEKCAGRVVFFVDEIDAVRSLPFSSDEFFAAVRE
                     FYNRRTEDPELSRLTFCLLGVATPSDLIRDVRTTPFNIGQRVELTDFTEEEALPLAEG
                     MNRAAGVGERLVRRVLHWTGGHPYLTQRLCLAVAEDETITDERGVDRLCEEMFLSPRA
                     RERDDNLLFVRERIVRSERLAGLLDLYSKVRAGAKVPDDETNPLVSVLRLSGIVRAVG
                     GQLRVRNRIYAHVFDKAWVVANMPDAELRRQRAAYRRGVLRAAGVAAVILVAMSVLAA
                     VAVGQRNRAVEEKNRADDSFRQLQKTYEALGRALKETKAQQEIADEQRRLAEEERLEA
                     DKQRQLAADGKQLAERESARAVAALKTAEAQRQLASRRAREAEEQRLAAERNRQTAEQ
                     NQVRAEGERARAEELSKLSIDTLFNITRFLESTNEKLRAVEYYEQLALIYRKAARKRE
                     EAETLLNIGDVYVKLKQAPKAAEYFERSLGVYRGAEAEEEGRTYVKIGNIYDISGDRA
                     RASSYYEQAIRHLQASLPALQTRKDQSARAGYALYYLSLAYEGLGDSRRALDYTRQAL
                     PLFRALGDSVMEAVLDIRLEELTQTVKDTP"
BASE COUNT          682 a         1509 c         1586 g          816 t
ORIGIN      
        1 gacgcggcgg ccccgccggc gccgcctctt cgtgctggcc gtgagcgtcc tgcgacccgt
       61 gaccgcccgc gcccgcgccc gccacgtcgt tgaccggagc cgggacagcc gcgctctcgt
      121 caaccgccgt cggcgacgcg cccgcgctcg ccgtgtgtga cgcgaccgac gtggtcgaag
      181 tggccgcgct cgccgccacc gccttcgcgc gctcggcctc aagctccttc acgcgcgcct
      241 caagctcttc gacgcggcgc agcatcgcgc gcatcgcctc gcgctcggct gcggtcagcc
      301 cgccttcggt cggcgccttg ctcgcctccg tcttcgaatc gtcgctcgac ttcttcgccg
      361 tcgcgttgtc cttcgagacg gcgggagcag aggcgtcggc ggggacgttg ccgccgaccg
      421 ccgaggccgg ctgctgccgc cgtgtcttga agaagatgtc gaggcggttg aagctctgcg
      481 cgaagctcgc ctcgacgccg tcgcggaggc ggaaggagag gcgcaggccg tcgccgtggc
      541 gctctgcgcg cgcgcgttcg aagccgccgc cctgaagctc gctcggccct tgtgcaagct
      601 cggcgtgcgg gagcgtgacg acgaatcggt cgccctcgcg ccgcgccgcg tagtcgtcga
      661 gcggcgcgtc cgaggcgacc gtgacgcgcg tgccctccgg cgtctccgac accttgagcg
      721 gggcgatgcg cttcagccgc agcggcttcg ccgtcgtcgt gttggcggtg atgagtcggg
      781 gctgcgcgga cgcgaactgc tccgccgtct gcgcgcgcag cgcgggcgcg gcgacgagca
      841 gcagggcgac gaaaagaagc gagcgacgag cacggcgccg ggggatgtcg ttcatatctg
      901 tgccctcgcc cgacgaggcc cgttcgcgtc cggcgagcgg aggatgccga ggaaggaaat
      961 gtttgtcgga gagactgacg aactctgaag attcgaagac cgttctcgca cctgacggcg
     1021 gtagcttatg tgagcgacca cggcaagtca acagccttga tttttcgata acgtcccgat
     1081 tcgtgttgcc gaaaaatccg gcggttgctc tctttgactt tcatgcttga aaaagccttg
     1141 cccgtgctgg gctttcgggg gaaacttatg gccgaagcag ccctgcgcga gcgaatcgat
     1201 atatatgtcc attccagtcg aatccttcga gagcgcctat tgggagcggg aaatcgcgga
     1261 ggccgtcgag actcccgacc cgtcgctctc caaccggaaa atcaccctcg tccattacag
     1321 gctctcgcaa gtcctgcaag ccgtgaccgg cgccgacgcg ggagccaact ttcacacgtg
     1381 ggccgtctgg ggttcgcgga aagcgggggt gacaatccgg caggaggatt tgggcgaggc
     1441 gatccgtaac gccacggtcg tcgccgggat cgtcggtttc atcgtcgggc tggccgtgtc
     1501 gtgggtgtcg ataagcctgt ggctcaacgg cctgcattgg ggcgtcgtcc ccctgtcggc
     1561 gctggccggg atgtgttgcg gcgcgtgggt cggtcggcgg atcgcgattt acagccggcg
     1621 cgaggccgcc cgtctgattc tggaaggcaa ccgcatcgtg ctggaggata tcggcagaca
     1681 gacggcgcgc ttcgtcgccc tgtttcacga caaagacgaa gaagacgcga gcgctctcga
     1741 cgcgttcctc gccgaactgc gtccgggcga gaccgcttcc ggcggacagg atttgctgcg
     1801 ccaggccttc acgcaatact acaccgcccg ctatgccgga gatttgaagc ggagacacga
     1861 ggccgcttac ttcgccaact gtctggcggt cttgcacgag cacatccggc tcgaacctta
     1921 catcaaaaag tcgatgcctt acatcatccg caagtgtgcg accaagcgcc tgatgcagtt
     1981 cgatgtcggg gcggtgagcc tgacggtgag cgaagaagtc ccacggctcg acggcgtgcc
     2041 tttccccgat tcgctgcacg atttatcgag tcgggaactc tccgattttc tcggcggcgc
     2101 acagggctgg ggcggcgacc tcgtgaaaag ccccgcgcgt gactggacga atttacgcga
     2161 gcggatgagg tatatcgttc agctcttccg cgtgatgcat ctggagccgt cggtctttgc
     2221 cgaaccgtac acgccgacgc agggtgagcg cccactgccc ttcgccgccg agttgccgag
     2281 gcggtaaacg aaaaacggaa aagtaacctc cggccagcga cacgaaattc aggacaccac
     2341 gcgctcagga cactacgcgc tacggcgtgt ccttgacggt ctgcgtcaac tcttcgaggc
     2401 ggatgtcgag gacggcctcc atgacggagt cgcccagtgc gcggaagagg gggagcgcct
     2461 ggcgcgtgta gtcgagcgcg cggcgcgagt cgccgaggcc ctcgtaggcg agcgaaaggt
     2521 agtagagcgc gtagcctgcg cgggcgctct ggtctttgcg cgtttgcagc gcgggcagcg
     2581 acgcctgcaa gtgtctgatc gcctgctcgt agtagctgct ggcgcgcgcc cggtcgccgg
     2641 agatgtcgta gatgttcccg atcttgacgt aggtgcgccc ctcctcttcg gcctcggcgc
     2701 cgcggtagac gccgagcgag cgctcgaagt attcggcggc cttcggcgcc tgtttgagct
     2761 tgacgtacac gtcgccgatg ttgagcaagg tctctgcctc ctcgcgtttg cgcgcggcct
     2821 tgcggtagat gagcgcgagc tgttcgtaat actcgacggc gcgcagcttc tcgttcgtgc
     2881 tttcgaggaa gcgcgtgatg ttgaagagcg tgtcgatgga gagcttgctc agctcttcgg
     2941 cgcgggcgcg ctcgccttcg gcgcgcacct gattctgttc ggcggtctgg cggttgcgct
     3001 cggcggcgag gcgctgctct tcggcctcgc gcgcgcgccg gctggcgagc tgccgctgcg
     3061 cctcggcggt cttgagcgcg gcgacggcgc gtgcgctctc gcgctcggcc aactgctttc
     3121 cgtcggcggc gagctgccgc tgcttgtctg cctcaagccg ttcttcttcg gcgaggcgcc
     3181 gctgctcgtc ggcgatctcc tgctgcgcct tcgtctcctt aagcgcgcgc ccgagcgcct
     3241 cgtaggtctt ctgcaactgg cggaagctgt cgtcggcgcg gttcttctcc tcgacggcgc
     3301 ggttgcgctg gccgacggcg accgccgcga gcaccgacat ggcgacgagg atgacggcgg
     3361 caacgcccgc cgcgcgcagc acgccgcgcc ggtaggccgc gcgctggcgg cgcagctccg
     3421 cgtccggcat gttggcgacg acccacgcct tgtcgaagac gtgcgcgtag atgcggttgc
     3481 ggacgcggag ctgtccgccc accgcgcgca cgatgccgga gaggcggagc acgctgacga
     3541 gcgggttggt ctcgtcgtcc ggcaccttgg cgcccgcgcg caccttcgag tagaggtcga
     3601 gcaggccggc gaggcgttcg ctccgcacga tgcgctcgcg gacgaagagc aggttgtcgt
     3661 cgcgctcgcg cgcgcgcggc gacaggaaca tctcttcgca gaggcggtca acgccgcgct
     3721 cgtccgtgat cgtctcgtcc tcggcgaccg cgaggcagag gcgctgcgtc aggtaggggt
     3781 gcccgcccgt ccagtggagc acgcgccgca ccagccgctc tccgacgccc gcggcgcggt
     3841 tcatcccttc ggcaagcggc agagcctctt cctcggtgaa gtccgtcagc tcgacgcgct
     3901 ggccgatgtt gaagggcgtg gtgcgcacgt cgcgaatgag gtcggagggc gtggcgacgc
     3961 cgagcaggca gaaggtcagg cggctcagtt ccgggtcttc cgtccggcgg ttgtagaact
     4021 cgcgcaccgc ggcgaagaac tcgtccgaag agaaggggag acttctgaca gcatcgatct
     4081 catcgacgaa gaagacgacg cgccccgcgc acttttcgag cacgacctcg cgcaccgcgc
     4141 gcgtccagcg ctgcaaaggc ccgacgcgct cgtgctcgtc ccagaagtct tccagctcgt
     4201 cttcgaggtc gagctgccgc ccgacgcggt tcagcagccc gtcgtaccac tgctcggcgg
     4261 agaggttctg cccgatggcc gtcaggtcga tgaccgccac cgtcacgccc tccgagcgca
     4321 gccgcgccgc cgtgctgacc atcaagctgg acttgcccat ctgccgcgag gtcaggacgt
     4381 agcagaactt gccctcgctc agcccttcgt acaggtcgtg gtcggcgcgg cgcgcgacgt
     4441 agctcggcgc gtcgcgtttg agcgtgccgc cggtgacgta gaagctcgat tgactcataa
     4501 cggtggtcgg tggtcggtgg ttgaatgtcc ggggtcatgc cgcatccggt ggagcggtcg
     4561 ggcgcttcaa gctacgtcgg cgaggtgagc aac
//
LOCUS       DBNGOH010000483         3742 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3004503, whole genome shotgun sequence.
ACCESSION   DBNGOH010000483 DBNGOH010000000
VERSION     DBNGOH010000483.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3742)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3742)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3742
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3004503"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(18..923)
                     /locus_tag="AC159P_09550"
     CDS             complement(18..923)
                     /locus_tag="AC159P_09550"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3695736.1"
                     /translation="MSSRKVARRTGEGDAMKEGEEAARPAGEGRVDGPLCAYPPGAGA
                     LHQQIESLVWELQRRTSELEEANLELSRVGRYRSLFLARMSHELRTPLTSILGFAEIL
                     LDQEDLTDPQRRFCEKIQASGLQLQASLTQLVDLSRIEAGQTELFLHEFSLRETLRES
                     CAAVARLAQKQEVTLECYPASGVGHIVSDEGKLRQVLYNFLAHAIGRSPNGGRVEVRA
                     AQADADSLVVEIADEGERLTDPSHLFEPVDIDAPNERGTNMNELGLVIAHRLLGVLGG
                     SATLHDLEPRGLAVRLNLPARPTEA"
     gene            complement(1148..2215)
                     /locus_tag="AC159P_09555"
     CDS             complement(1148..2215)
                     /locus_tag="AC159P_09555"
                     /EC_number="7.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002633634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006119 - oxidative phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HIR3695737.1"
                     /translation="MSLEVLVAGTMIVSLTFYALLGGADYGGGVWDLFARGPRAKEQR
                     ELIAHAIGPVWEANHVWLILVVVILFTAFPTAFAVIATALHVPLTLLLVGIVLRGAAF
                     TFRTYDSQRDDVQRRWSYVFSIASTITPVLLGVALGAVASGRIRVENGVVTTGFWWSW
                     LAPFPFAVGLFALALFAFLAAVYLTLEARGGELQEDFRRRALVSGAVVGLLALTVFVL
                     AGDGAPKVRAGIWASAWALPLHVATGVCAVGALYTLWARRYALARLFAAAQVTFILWG
                     WALAQFPAIVEPDITIRTAAAPRITLQLLVAALAVGALVLFPSFFYLFRVFKGHTAFA
                     PLAGEPGEISDTAIKTGESAR"
     gene            complement(2205..3560)
                     /locus_tag="AC159P_09560"
     CDS             complement(2205..3560)
                     /locus_tag="AC159P_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HIR3695738.1"
                     /translation="MDDLMAARSQMAMSLAFHIIFAAIGIAMPLLMVVSEWRWLRTRE
                     EVYLTLAKRWAKGTAILFAVGAVSGTVLSFELGLLWPGFMGYAGAIIGMPFSLEGFAF
                     FTEAIFLGIYLYGWERVPPRAHLLAGVAVAVSGALSGVFVVIANAWMNSPTGFELVDG
                     RPANIDPIAAMLNPMAFSQTLHMTIAAYAATGFVVAGVHAYLLLRDRSNLFHRRALAI
                     ALAVGGAAALLQPLSGDLSARAVAEYQPAKLAAAEAHFKTEAGAPLRVGGIPDEETGE
                     VAYSIEIPYGLSLLAFHDPEAVVKGLDAFPRDQWPNTLIVHTAFQIMVGLGMLMMALA
                     LWGAFLWWRKRSLPDEKWFLRALVAAAPAGMIAIEAGWTVTEVGRQPWIIQGVMRTSE
                     AVTPMQGLVVPFVFFTLLYVFLAVAVVWLLLRQVAASPRIIREGNEEAGAEKGGVLDV
                     A"
BASE COUNT          673 a         1295 c         1236 g          538 t
ORIGIN      
        1 ccccttctcc ccactgccta cgcttccgtc gggcgggccg ggaggttgag gcggacggcg
       61 aggccgcgcg gttcgaggtc gtggagcgtg gccgagccgc cgaggacgcc gagcaggcgg
      121 tgcgcgatga cgaggccgag ttcgttcatg ttggtgccgc gctcgttggg cgcgtcgatg
      181 tccaccggct cgaagaggtg cgaggggtct gtcaggcgct cgccctcgtc cgcgatctcg
      241 acgacgaggc tgtcggcgtc ggcttgcgcg gcgcgcacct cgacgcgccc gccgttgggg
      301 ctgcgaccga tggcgtgcgc gaggaagttg tagagcacct gccgcagttt cccttcgtcg
      361 gagacgatgt ggccgacgcc cgacgccgga taacattcga gcgtcacctc ctgcttctgc
      421 gcgaggcgcg cgaccgccgc gcaggactcg cgcagcgtct cgcgcaggga gaactcgtgg
      481 aggaacagct cggtctgccc ggcctcgatg cgcgagagat cgacgagctg cgtgaggctg
      541 gcttgcagtt gcaggcccga cgcctgaatc ttctcgcaga agcggcgctg cgggtcggtg
      601 aggtcttcct ggtcgagcag gatttcggcg aagccgagga tcgaggtcaa gggcgtgcgc
      661 agctcgtgcg acatgcgcgc caggaagagc gagcggtagc ggcccacccg gctcagttcg
      721 aggttggcct cttcgagttc cgaggtgcgc cgctgaagct cccacaccag gctctcgatc
      781 tgctggtgga gcgcgcccgc gcccggcggg tacgcgcaca aggggccgtc cacccgcccc
      841 tcgcccgcgg ggcgcgcggc ctcctcgccc tccttcatcg cgtcaccctc gcccgtccgt
      901 cttgccactt tgcgcgacga cacaaaaaac ctccctccac tcgctcacgg gaagagacct
      961 tgctgcccca gattctttcg agtttcttaa ctgccgcgaa ctataaatcc acgaccgtac
     1021 gcaggtcaaa ccgccgcccg ccggagaggc aaaaggcgcg cgggccgtat gctgagaagt
     1081 aaaaaaaatt tcggactcgg agccgtccgt gctccgagtc cgaatgtatc ccccgccggc
     1141 atcgattcta tctcgcgctt tcgcccgtct ttatcgcggt gtcgcttatc tcacccggtt
     1201 ctccggcgag cggcgcgaat gctgtgtgcc ctttgaacac tcggaagagg tagaagaacg
     1261 aagggaacag aacgagcgcc ccgacggcga gggcggcgac caacagttgg agtgtgatgc
     1321 ggggcgccgc agccgtccgg atggtgatgt ccggctccac gatggccggg aactgcgcca
     1381 acgcccagcc ccacagaatg aacgtgacct gcgccgccgc gaacagacgc gcgagcgcgt
     1441 accgcctcgc ccagagtgtg taaagggcgc cgacggcgca cacgcccgtc gcgacgtgga
     1501 gaggcagcgc ccacgcgctc gcccaaatcc cggccctcac cttcggcgcc ccgtcgcccg
     1561 ccagcacgaa gaccgtcagc gcgagcaggc cgaccaccgc cccggagacg agtgcgcggc
     1621 ggcggaagtc ctcctgcaac tcgcccccgc gggcctccag tgtgagatag acggcggcca
     1681 ggaacgcgaa cagcgcgagc gcgaaaagac cgaccgcgaa ggggaacggc gcgagccacg
     1741 accaccagaa gcccgtagtg acgacgccgt tctcgacacg tatccggccc gaagcgacgg
     1801 ccccgagcgc caccccgagc aggacgggcg tgatcgtgct cgcgattgag aagacgtagc
     1861 tccagcggcg ctgcacgtcg tcgcgctgcg aatcgtaggt gcggaaggtg aaggccgcgc
     1921 cgcgcagcac gatgccgacc aagaggagcg tgagcgggac gtggagcgcc gtcgcgatga
     1981 ccgcgaaggc tgtggggaag gccgtgaaga ggatgacgac gacgagtatc agccagacgt
     2041 gattggcctc ccacacaggc ccgatggcgt gcgcgatcag ctcgcgctgc tctttcgccc
     2101 gcggcccgcg cgcgaacaaa tcccacaccc cgccgccgta gtccgcgccg ccgagcagcg
     2161 cgtagaaggt gagcgacacg atcatcgtcc cggcgacgag cacttcaagc gacatcgagc
     2221 acgcccccct tttcggcccc ggcctcttcg ttcccttccc tgatgatgcg cggactcgcc
     2281 gccacctgcc gcagcagcag ccagacgacc gccaccgcga gaaagacgta cagcagcgtg
     2341 aagaagacga agggcacgac gaggccctgc atcggcgtca ccgcctcgct cgtccgcatc
     2401 acgccttgaa tgatccacgg ctggcggccc acctccgtca ccgtccaccc cgcctcgatg
     2461 gcgatcatcc ccgccggggc cgccgcgacg agcgcgcgca ggaaccactt ctcgtcgggg
     2521 agactccgct tgcgccacca gaggaacgcg ccccacagcg ccaacgccat catcaacatc
     2581 ccaagcccga ccatgatttg aaatgccgtg tggacaatca acgtgttggg ccactggtcg
     2641 cgcgggaaag cgtcgagacc tttgaccacg gcctcggggt cgtggaacgc gagcagactg
     2701 aggccgtacg gaatctcgat ggaatatgcg acctcgccgg tctcctcgtc ggggatgccg
     2761 ccgacgcgca gcggagcgcc ggcctcggtc ttgaagtgcg cttcggctgc cgcgagtttt
     2821 gccggttggt actcggccac ggcccgcgcg ctcaggtcgc cgctcagggg ctgaagcagc
     2881 gccgccgcgc cgcccaccgc cagcgcgatg gcgagcgcgc ggcggtggaa gaggttggag
     2941 cggtctctga gcaaaaggta tgcgtggacg cccgcgacga cgaagcccgt ggcggcgtac
     3001 gcggcgatgg tcatgtgcag cgtctgcgag aaggccatcg ggttgagcat cgcggcgatg
     3061 gggtcgatgt tggcgggcct gccgtccacc aactcgaacc ccgtcgggct gttcatccac
     3121 gcgttggcga tgacgacgaa gacgcccgac agcgcgccgc tcacggccac cgccacgccc
     3181 gccagcaggt gcgcgcgcgg cggcacgcgc tcccagccgt agaggtagat gccgaggaag
     3241 atcgcctctg tgaagaaggc gaagccttcg agcgagaagg gcatcccgat gatcgcgccc
     3301 gcgtagccca tgaagccggg ccagagcagc cccagctcga acgagagcac cgtgcccgag
     3361 acggcgccga ccgcgaagag gatggccgtt cccttcgccc aacgcttggc gagcgtcagg
     3421 tagacctcct cgcgcgtgcg cagccagcgc cactcggaga cgaccatcag cagcggcatc
     3481 gcgatgccga tggccgcgaa aatgatgtga aaggcgagcg acatcgccat ctgcgagcgc
     3541 gccgccatca ggtcatccac tactcagccc ctccgcccac gactcgacta atcacgtctg
     3601 acgaaagttt atcagcagcg cccgcccgcg ccgtcatgtg ccgaagggtg ccgcacatga
     3661 aatcttttcc ggccccgcgc ctgccattct tataccgact tgcgaatccg attaagattc
     3721 atcagccgcg gatgaacgcg ga
//
LOCUS       DBNGOH010000484         3523 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4286768, whole genome shotgun sequence.
ACCESSION   DBNGOH010000484 DBNGOH010000000
VERSION     DBNGOH010000484.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3523)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3523)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3523
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4286768"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2359)
                     /locus_tag="AC159P_09565"
     CDS             complement(<1..2359)
                     /locus_tag="AC159P_09565"
                     /inference="COORDINATES: protein motif:HMM:NF024816.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HIR3695739.1"
                     /translation="MTLRTVVLALMLALAHGLAGAGLAPAQQPAAQGGPRLVGVRLGT
                     DATPDLDIIDPSAAFSPDTPQLVCVWSVADIPAGGTTIKGVWVAEDTGGAAPPNYVIG
                     ETPYELPGPGPMSGRFDLSKPTRGWPPGKYRLELHLAGRLAHTITFRIMTAAEAEVYD
                     VQLAPLLKRADELEKTGDYKQAAALTGQALAVAEKNLGPEHPDTAALVNNLALLLKEA
                     GDYARSEELYRRAVALAEKLGGAEQPAFAVTLSNLAELERLKGDYPRSESLLARALPV
                     MEKALGAEHPFVAVILNNQALNAQAKGDFERARTLYERALSITEKARGPEHDDVAMIL
                     NNLAEAYRERNDYARAEPLYRRALAVREKSLPADHPTVALTVNNLALLYQERGDHARA
                     EPLFRRALAAYEKTLGENHADVATLLNNLALVYEAAGDAARAEAAYRRAARIAEQALG
                     GRHPTLARVTYNTALLAAERGDFKQSLELLARAADIREQNISLVLSTGSEQQKQTFLD
                     TLYGETNLTLSFHLRTLPADAGAARLALETLLRRKGRALDVMSDQIGSLRRRLKPEDR
                     QLLDRLSNARSRLAALVLAGGGADAGARQEEVKRLDAEVQRLEDEVGARSSGGSEELR
                     AGANVFARAATVERVQAAIPADAALVELVRYHPFDFKAQRGAAFGAARYAAYVVRREG
                     APAWVELGDAAAIEAGVREMRAAVSTPARADAKQAARALDEIAARPLRKLLGETRRVF
                     LSPDGALNLVPFAALMDEGGRYLVESYAFTYLTSGRDLLRLRADTP"
     gene            2736..>3523
                     /locus_tag="AC159P_09570"
     CDS             2736..>3523
                     /locus_tag="AC159P_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014101067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HIR3695740.1"
                     /translation="MSAIIDTPPTADIERGTEEVETVTIRFAGDSGDGMQLTGTQFTN
                     TSAIVGNDISTLPDFPAEIRAPAGSLPGVSGFQLNFSSRDIRTPGDQPNVLVAMNPAA
                     LKVNLSDLEDGGTIIVNTDEFNKENLKKANYAANPLEDETLKGYRIHRLPITTLNIRA
                     LQEAGVKLSRKEMDRCKNFFALGVLYWLYDRPMEPSLEWIKAKFAKNEEVARANEVAL
                     KTGYNFADTTEVFTTHYTVKKAVIAPGKYRKITGNEATAIGFIAA"
BASE COUNT          532 a         1209 c         1239 g          543 t
ORIGIN      
        1 tgggcgtgtc cgcgcgcagc cgcagcaggt ctcgaccgct cgtcaggtag gtgaaggcat
       61 aggactcgac gaggtagcgt ccgccctcgt ccatgagggc ggcaaagggg acgaggttga
      121 gcgcgccgtc gggcgagagg aagaccctgc gcgtctcgcc taagagcttg cgcaaagggc
      181 gcgcggcgat ttcgtcgagc gcgcgcgcgg cctgcttcgc gtcggcgcgc gcaggcgtag
      241 agacggcggc gcgcatctcg cgcaccccgg cttcgattgc cgccgcgtcg cccagctcga
      301 cccacgcggg cgcgccctcg cgccgcacga cgtaggccgc gtaacgcgcc gcgccgaagg
      361 ccgcgccccg ctgcgccttg aagtcgaagg ggtgatagcg gacaagctcg acaagcgcgg
      421 cgtccgccgg tatcgcggcc tgcacgcgct cgacggtcgc ggcgcgcgcg aacacgttcg
      481 cgcccgcgcg cagctcttcg gagccgccgc tgctgcgcgc gcccacttcg tcttcgagcc
      541 gctgcacctc cgcgtcgagc cgcttgacct cctcctgccg cgcgcccgcg tccgcgccgc
      601 cgcccgcgag cacgagcgcc gcgagccgcg agcgcgcgtt cgacaaccta tcgagaagct
      661 gccggtcttc gggcttcagc cttcggcgca ggctcccgat ctggtcgctc atcacgtcga
      721 gcgcgcggcc cttgcggcgg agcagcgtct ccagcgcgag gcgcgcggcc cccgcgtcgg
      781 cgggcagcgt gcgcaggtgg aaggagagcg tcaggttcgt ctcgccgtag agcgtgtcga
      841 ggaaggtctg cttctgctgc tcggagccgg tcgagaggac gagcgagatg ttctgttcgc
      901 ggatgtcggc ggcgcgcgcg agcagttcga gcgactgttt gaagtcgccg cgttcggcgg
      961 cgagcagcgc cgtgttgtag gtgacgcgcg cgagcgtcgg gtggcgaccg ccgagcgcct
     1021 gttcggcgat gcgcgccgcg cgtcggtagg cggcctctgc gcgcgccgcg tcgcccgccg
     1081 cttcgtagac gagcgcgagg ttgttcagca gcgtcgccac gtcggcgtga ttctcgccga
     1141 gcgtcttctc gtaagcggcg agcgcgcggc ggaacagcgg ctcggcgcgc gcgtggtcgc
     1201 cgcgctcctg gtagagcagc gcgaggttgt tgaccgtcag cgcgaccgtc gggtggtcgg
     1261 cgggcagcga cttctcgcgg acggcgagcg cgcgtcggta gagaggctct gcgcgcgcgt
     1321 agtcgttgcg ctcgcggtag gcttcggcga ggttgttgag gatcatcgcc acgtcgtcgt
     1381 gctcggggcc gcgcgccttc tccgtgatcg agagcgcgcg ttcatacaac gtgcgtgcgc
     1441 gctcgaagtc gcccttcgcc tgcgcgttca gagcctggtt gttgaggatg acggcgacga
     1501 acgggtgctc cgcgccgagc gccttctcca tgacgggcag cgcgcgcgcg agcagagact
     1561 cggagcgcgg gtagtcgccc ttcagccttt cgagttcggc gaggttgctg agcgtcacgg
     1621 cgaaggcggg ctgctccgcg ccgcccagct tctctgcgag ggcgacggcg cggcggtaca
     1681 gctcttcgct gcgcgcgtag tcgcccgcct ctttgagcag cagcgcgagg ttgttgacga
     1741 gggccgccgt gtccgggtgc tcggggccga gatttttttc cgcgacggcg agcgcctgcc
     1801 ccgtcagcgc ggcggcctgc ttgtaatcac cggtcttctc aagctcgtcg gcgcgcttca
     1861 ggagcggcgc gagctgcacg tcgtaaactt cggcctccgc cgccgtcatg atgcggaaag
     1921 tgatggtgtg cgcgagcctg cccgcgaggt gaagctcaag ccggtacttg ccgggcggcc
     1981 agccgcgcgt cggcttcgag aggtcgaagc ggcccgacat cggccccggc cccggcagct
     2041 cgtaaggcgt ctcgccgatg acgtagttcg gcggggccgc gccgccggtg tcctcggcca
     2101 cccacacgcc cttgatcgtc gtcccgcccg cggggatgtc cgcgacgctc cacacgcaga
     2161 cgagctgcgg cgtgtcgggc gagaaggcgg cggaagggtc gatgatgtcg aggtcgggcg
     2221 tggcgtcggt gccgagcctg acgccgacga gacgcgggcc gccctgcgcc gcaggttgtt
     2281 gagcgggcgc gaggcccgcc cccgccagtc cgtgcgcgag cgcgagcatc agggccaaca
     2341 cgactgttcg aagtgtcatc tctcctacct tccccgccgc cgcccgcggc ggcccgcatt
     2401 gtagaccccc gcaccgcgac cgcgaaagcc gtgtcgggcg cgggcggcgt gatgcatcgc
     2461 ggagggcggg gcgagcgccc gcgctggcat ggggtttgtc ttcgggttgt ttgtcgcgcg
     2521 gcggagcgtg aagatgggag aaattcgggt gctttcaccc accgccgtgt gaaattcccg
     2581 ccgcggcgcg cgcgctctgt gggacgcgcc gcggctatgt tagaatcctt gcgaagcccg
     2641 aaagaggccg aagtgtcgag cacgcgggtt cgccgccggt gattcccagc accaaacatc
     2701 ccgaccaccc gaagccgaag gattgaacga gagagatgag cgccatcatt gacacgccgc
     2761 caaccgccga catcgagaga gggacagagg aagtcgagac cgtgaccatc cgcttcgccg
     2821 gcgactccgg cgacggcatg cagttgacgg ggacgcagtt caccaacacc tccgccatcg
     2881 tcggcaacga catctcgacg ctgcccgact tccccgccga gattcgcgcc cccgccggct
     2941 ccttgcccgg cgtctccggc ttccagttga atttttcgag ccgcgacatc cgcacgcccg
     3001 gcgaccagcc gaacgtgctc gtcgccatga acccggcggc cctgaaggtg aacctctccg
     3061 acctcgaaga cggcggcacc atcatcgtca acaccgacga gttcaacaag gagaacctga
     3121 agaaggccaa ctacgcggcg aacccgctcg aagacgagac gctgaaaggc tatcgcatcc
     3181 accgcctgcc gatcacgacc ttgaacatcc gcgccttgca ggaggcgggc gtcaagctct
     3241 cgcgcaagga gatggatcgc tgcaagaact tcttcgcgct cggcgtcctc tactggctct
     3301 acgaccgccc gatggagcct tcgctcgaat ggatcaaggc caagttcgcg aagaacgaag
     3361 aggtcgcgcg cgccaacgag gtggcgttga agaccggcta caacttcgcc gacacgaccg
     3421 aggtcttcac cacccactac acggtcaaga aggccgtcat cgcgcccggc aagtaccgca
     3481 agatcacggg caacgaggcg accgccatcg gcttcatcgc cgc
//
LOCUS       DBNGOH010000485         3504 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1538383, whole genome shotgun sequence.
ACCESSION   DBNGOH010000485 DBNGOH010000000
VERSION     DBNGOH010000485.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3504)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3504)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3504
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1538383"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..507)
                     /locus_tag="AC159P_09575"
     CDS             complement(<1..507)
                     /locus_tag="AC159P_09575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003162065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HIR3695741.1"
                     /translation="MHYHLIGICGTAMASLAAMLKERGHRVTGSDENVYPPMSTMLDA
                     LNIPVMQGYKPEHLAAAPDCVVVGNALSRGNPEVEETLNRRLTYRSAAEVIKEEFIRG
                     RRSLVVAGTHGKTTTTSIAAWVLERGGLNPGFLVGGIVQNFGASARVTDSPYFVIEGD
                     EYDTAYFDK"
     gene            complement(522..878)
                     /locus_tag="AC159P_09580"
     CDS             complement(522..878)
                     /locus_tag="AC159P_09580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695742.1"
                     /translation="MSPRIIWSRVEEKERRVRKLLERWRGGVAVISGAQVSHKWLILS
                     VDARDKFPRFLEIYCGDCEYVAGPFKWAKSAFELERRERGSEEDFTYILRDESNGFTV
                     HCGIVEAKEKSYSDLK"
     gene            complement(881..2236)
                     /locus_tag="AC159P_09585"
     CDS             complement(881..2236)
                     /locus_tag="AC159P_09585"
                     /inference="COORDINATES: protein motif:HMM:NF024970.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR3695743.1"
                     /translation="MEGITSTRLGDIYVRREADIVKVRERVRGLAREMGFDPTTQIKI
                     TTAVSELTRNIYEYAKSGAITLAVAERGAAATGIQITARDDGPGIEEARLRAIARGTY
                     RSPSGLGVGLVGTRRLMDEFDIQSKPGEGTRVTVVKWLPTAEAEEVRERMDALRASVA
                     VEEDDSAIEELSRQNRDLVQILSELEEKREQLEQVNRRLEESNRELNEANEKLRELSD
                     MKEEFLALTTHDLRSPLTVISGVINFFTSGRLGDMTPEQKNMVAMMERNTQSLIELVN
                     DLLDASKLESGTLRLEVAPVELAPLVAELRETMLPMAKEKEIALEEHVAADLPAVSAD
                     RAKLRRVLVNLLSNAVKFTPKGGRIEVNAERDGKRAVRVSVTDTGVGIPPEDLARLFD
                     KFEQARSRATRSEKGTGLGLYITRQLVELHGGEINVESEVGKGSTFSFTIPVADRDEG
                     S"
     gene            complement(2411..>3504)
                     /locus_tag="AC159P_09590"
     CDS             complement(2411..>3504)
                     /locus_tag="AC159P_09590"
                     /inference="COORDINATES: protein motif:HMM:NF024970.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR3695744.1"
                     /translation="SNPQSAIRNPQFFMRDFQSQIITDEAQVGTARRAVERYARGLGF
                     TDDELAEIAIVVQEIGTNAARYASEGGHLHWTTPPGDARGLELFYCDKGPGIHDFERA
                     ARDGVSSGGSLGAGLGAVRRLLDEFDAYSTVSGLTRRLPGSRRTTHGTAILGRKWPAS
                     ARHRAEREEQKLMRRRLGVCSRPRPGEDTNGDAYFVAEGDGETLFAVIDGLGHGTGAR
                     QASDAALGALEGWDGQPLDEVVWTVHEALRSTRGAVIGAVVLDRARGTFTYAGVGNIE
                     VRVLGAAEAARPIPSNGTLGARLSQVRVWPHRWAEGMSIVMASDGLSASWDIESYPGI
                     LKQSPQLLAGILLRDYGRDSDDATVLVIR"
BASE COUNT          521 a         1198 c         1173 g          612 t
ORIGIN      
        1 cttgtcgaag taggccgtgt cgtactcgtc gccctcgatg acgaaatagg ggctgtcggt
       61 cacgcgcgcg ctggcgccga agttctgcac gatgccgccg acgagaaaac cggggttcag
      121 cccgccgcgc tccagcaccc acgccgcgat gctcgtcgtc gtcgtcttcc cgtgcgtgcc
      181 cgcgacgacg agcgagcggc ggccacggat gaactcctcc ttgataacct ccgccgccga
      241 gcggtacgtg agccgccggt tcagcgtctc ttcgacctcc gggttgccgc gcgacagggc
      301 gttgccgacg acgacgcagt ccggcgccgc cgcgaggtgc tccggcttgt aaccctgcat
      361 gaccgggatg ttcagcgcgt cgagcatcgt ggacatcggc gggtagacgt tctcgtccga
      421 gccggtcacg cggtgcccgc gctccttcaa catcgcggcc agagacgcca tcgcggtgcc
      481 gcagattccg atgaggtggt agtgcataag cggggcggaa attacttcaa gtcagaatag
      541 gatttctctt tcgcctcgac gatgccgcag tgcacggtaa agccgttgga ctcgtcgcgc
      601 aggatataag tgaagtcctc ctcgctccca cgctcccgcc tctccaactc gaaggcactc
      661 ttcgcccact tgaacggccc cgcgacgtat tcgcagtcgc cgcagtaaat ctcaagaaag
      721 cgggggaatt tatcgcgggc gtcaacgctc agtatcaacc acttgtgact gacctgcgcg
      781 ccggaaatca cggccacccc gcctcgccac ctttccagca gcttgcggac gcgtctttcc
      841 ttctcctcga cgcggctcca gattatccgc ggactcatga ttacgacccc tcgtcccggt
      901 cggccacggg gatcgtgaaa gagaacgtgg aacccttgcc gacttcggac tcgacgttga
      961 tctcgccgcc gtgcagctcg acgagctggc gcgtgatgta caggccgagg ccggtgccct
     1021 tctcgctgcg ggtggcgcgc gagcgggcct gctcgaactt gtcgaagagg cgcgcgaggt
     1081 cttcgggcgg gatgccgacg ccggtgtccg tgaccgagac gcgcaccgcg cgcttcccgt
     1141 cgcgctcggc gttgacctcg atgcgcccgc ccttgggcgt gaacttcacc gcgttcgaga
     1201 gcaggttgac gaggacgcgg cggagcttgg cgcggtcggc gctgacggcg ggcaagtccg
     1261 cggcgacgtg ctcttcgagc gcgatctcct tctccttcgc catcgggagc atcgtctcgc
     1321 gcagctcggc gacgaggggc gccagctcga ccggggcgac ttcgagacgc agcgtgccgg
     1381 attcgagctt cgaggcgtcc aaaaggtcgt tgaccagctc gatgaggctc tgcgtgttgc
     1441 gctccatcat ggccaccatg ttcttctgct cgggcgtcat gtcgccgagg cggcccgagg
     1501 tgaagaagtt gatgacgccg ctgatgaccg tcagcggcga gcgcaggtcg tgcgtcgtga
     1561 gcgcgaggaa ctcctccttc atgtccgaca gctcgcgcag cttttcgttc gcctcgttca
     1621 gctcgcggtt gctctcttcg agccggcggt tgacctgttc gagctgttcg cgcttctcct
     1681 caagctcgga gagtatctgc acgaggtcgc ggttctgccg cgacaactcc tcgatggccg
     1741 agtcgtcctc ttcgaccgcc accgaggcgc gcagcgcgtc catgcgttcg cgcacctcct
     1801 cggcctcagc cgtcggcaac cacttgacga ccgtgacgcg cgtgccctcg cccggcttcg
     1861 actggatgtc gaactcgtcc atcaggcggc gcgtgccgac gaggccgacg cctagccctg
     1921 aaggcgagcg gtaggtgccg cgcgcgatgg cgcgcaggcg cgcctcctcg atgccggggc
     1981 cgtcgtcacg cgccgtgatc tggatgccgg tcgcggcggc gccgcgctcg gcgacggcga
     2041 gcgtgatggc gcccgacttg gcgtactcgt agatgttgcg cgtgagttcc gagacggcgg
     2101 tcgtgatctt gatctgcgtc gtcgggtcga agcccatctc gcgcgccagc ccccgcacgc
     2161 gctcgcgcac cttcacgatg tcggcctcgc gccgcacgta aatatcaccc agtcgggtcg
     2221 aggtgatgcc ttccatagga ttcgaaagcc gtgaaccgtg aaccgtgaac cgtgacgagg
     2281 aagaagcgtg aaccatgatg cgtgaaccgt gagacggaag cacttcctct cggcctttag
     2341 atttaccacg tcggttaagc gcggctcaac cgctgttcac ggtttacgat ttacggttca
     2401 cggtctttag tcacctaatc accagcacgg tggcgtcgtc cgagtcgcgc ccgtagtcgc
     2461 ggagcaggat gccggcgagg agctgcgggc tttgcttcag gatgccgggg taggactcga
     2521 tgtcccagga ggcgctgagg ccgtccgagg ccatgacgat gctcatcccc tcggcccagc
     2581 ggtgcggcca gacgcgcacc tgcgagaggc gcgcgccgag cgtgccgttc gaggggatgg
     2641 ggcgcgcggc ctcggcggcg ccgagcacgc gcacctcgat gttgccgacg cccgcgtagg
     2701 tgaacgtgcc gcgcgcgcgg tcgagcacga ccgcgccgat gacggcgccg cgcgtcgagc
     2761 gcagggcctc gtggacagtc cacaccacct cgtccagcgg ctggccgtcc cagccttcga
     2821 gcgcgcccag cgcggcgtcc gacgcctgcc gcgcgccggt gccgtggccg aggccgtcga
     2881 tgacggcgaa gagcgtctcg ccgtcgccct cggcgacgaa gtacgcgtcg ccgttcgtgt
     2941 cctcgccggg gcgcgggcgg ctgcacacgc cgagccgccg ccgcatcaac ttctgctcct
     3001 cgcgctcggc gcggtgacgc gcggaggcgg gccactttct gccgagaatg gccgtgccgt
     3061 gcgtcgtgcg gcgcgagccg ggcaggcgcc gcgtcagccc gctcacggtc gagtaggcgt
     3121 cgaactcgtc gagcaggcgg cgcaccgcgc cgagtcccgc gccgagactg ccgcccgacg
     3181 agacgccgtc gcgcgccgcg cgctcgaagt cgtggatgcc cggccccttg tcgcagtaga
     3241 aaagctcaag cccccgcgcg tcgccgggcg gcgtcgtcca gtgcaggtgc ccgccctcgc
     3301 tcgcgtagcg ggccgcgttc gtgccgatct cctgcacgac gatggcgatc tcggccagct
     3361 cgtcgtccgt gaaaccgagg ccgcgcgcgt agcgctcgac cgcgcgccgc gcggtgccga
     3421 cctgcgcctc gtccgtgatg atctgtgatt ggaaatcccg catgaaaaat tgcggattgc
     3481 ggattgcgga ttgcggattg gaag
//
LOCUS       DBNGOH010000486         6766 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1538447, whole genome shotgun sequence.
ACCESSION   DBNGOH010000486 DBNGOH010000000
VERSION     DBNGOH010000486.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6766)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6766)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6766
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1538447"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..970
                     /locus_tag="AC159P_09595"
     CDS             <1..970
                     /locus_tag="AC159P_09595"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012497.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HIR3695745.1"
                     /translation="VTGPLELMGAHVKTEDGHAPLVVEGRSPLRAIEYETPVASAQVK
                     SCVLLAGLFAEGRTSVTEPHALTRNHTERMLRWFGAHVETGHAVRADGSTAHVHSVEG
                     VQGFGARDIDVPGDISSAAFMASAAALLPGSELIIEGVGLNPTRSRFLDTLVMLGADA
                     RVEDERDVCNEPVGTVRVRGARGAAEALDRGKPALLLRGEMIAQLIDELPVLAVVGTQ
                     VEGGIEIREAKELRVKESDRIRTTVDNLRAMGARVEEFEDGLKVHGRARLRGARLDAH
                     GDHRIAMAFAVAALVAEGETEIVGAEECVAVSFPEFFPLLESVTER"
     gene            973..1491
                     /locus_tag="AC159P_09600"
     CDS             973..1491
                     /locus_tag="AC159P_09600"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF013375.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HIR3695746.1"
                     /translation="MTTTPRIVITGFMGAGKTTVAAAIARLLGCAAIDLDDRITAREG
                     RTPQQLIDEDGEAAFRDVETRALGEVLGEEAARVVALGGGAWALERNRLLVCEHGCLA
                     VWLDAPFELCWRRIIEDSGEDRPNARDPSRARALYDARRHAYSLAALRVCVTPERSAD
                     DIAAEILSTPRA"
     gene            1591..3102
                     /locus_tag="AC159P_09605"
     CDS             1591..3102
                     /locus_tag="AC159P_09605"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF013319.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HIR3695747.1"
                     /translation="MGDRDTDAKGSKPAKELRNAHNWKHLARRHGLGYYELQTDDTGD
                     VPVRLFLTPQLLSDAEDILYRQIVNATRFPGVRMVVITPDTHYGYGVPVGCVLITDAD
                     AGAVAMGPVGYDIGCGMMSARSGVEAERATPEKRLEFNRAVMGRVDMGFGGKSKRLGR
                     LSEDEFQNLVRGGAEYYVEKYGATFDRSRAERHRIPVDDDWQIPWGGKGRPERGLEQI
                     GSLGGGNHFIELQRSEQTGTLFLQVHTGSRGFGHGLATNYFELAREERPEGVKDIDLG
                     YFTPESRHYRDYQNAVAAGGNYAILNRLVIFEQVSEAFREVFGEELELIYEISHNLVQ
                     REQHPEFGDVWVHRKGATRAFPAGHPALAGTVWEGTGHPVLIPGSNQDWSYILRPLEG
                     AVRSCYSVYHGAGSRMSRGEAARTLSQRAVDDEYREAGVLVNADGRVPIDEAAACYKP
                     AEEVIEAVVGAGLAEVEHRLWPLASLKGVDEAKNRKRRKGKGGVLGKKSGEHY"
     gene            3168..3812
                     /locus_tag="AC159P_09610"
     CDS             3168..3812
                     /locus_tag="AC159P_09610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695748.1"
                     /translation="MSKRTLFVCGVALGLALSALTLTAAEQAGVSGAAERREVEQRVT
                     GGVVTLYALDPLARTLCFADGRDGHVFHENEVRNRCSDLDFGSYRAGHFSVGVEGARL
                     GAVVNLGTADELRQRYGYPETVGNGQGFASLRAEGGRVLVLKGVRPHAEQELKESALL
                     FGEGAGAASAPVKLGNIYLVRLTDRRDRDFERVVKLKVIAHTPNESVTVRWQAL"
     gene            complement(3841..4896)
                     /locus_tag="AC159P_09615"
     CDS             complement(3841..4896)
                     /locus_tag="AC159P_09615"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF013215.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IB"
                     /protein_id="HIR3695749.1"
                     /translation="MVGSVTRGSRANWWRRRGTKRSGFRYETADGARVREEAALERIR
                     ALAIPPAWREVRISPSPRNSLQAIGVDAKGRAQRIYHPTTVARRQRHKFEKVERFGEA
                     LPALRRRANEDIVREGLTKERVLAVVVRLINDLYFRVGSEESVRRYRTYGVTTLRNRH
                     LDIQPGGRLCFDFVGKHRVKHRRVMVDEELAALMRDIKSIGGAKLFNYLGEDGKARPV
                     TPRDVNDYIKGATGPEFSAKDFRTWGGTLMAAIALAEIGCCEDRREARKNVARAVARV
                     AEHLGNTPTVCRGSYIHPAVLDRYVKGQTLEEFRPRKLRRVTRSQPDYEVEELALLKL
                     LRARKNGNGAAQGDGAR"
     gene            complement(5143..5631)
                     /locus_tag="AC159P_09620"
     CDS             complement(5143..5631)
                     /locus_tag="AC159P_09620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695750.1"
                     /translation="MATRKATKGASKKSSGKASATATPSAQTAQACAPGVCDPQRTQG
                     LKAPAPKGAAPQVLVNLEGPGVFLGAHVSKQGGDTDLTFVILDIDGRNVTNISFAAAR
                     NVGFTQQNPYGLVLLQSGAGIENLTIGFPTPLSYRRQLKLSVVVNENGVVQILGNVIH
                     GR"
     gene            complement(5764..6510)
                     /locus_tag="AC159P_09625"
     CDS             complement(5764..6510)
                     /locus_tag="AC159P_09625"
                     /inference="COORDINATES: protein motif:HMM:NF049746.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HIR3695751.1"
                     /translation="MKAELEQLIALQETDTAIRRLQAELDAIPRRRAEIEAEFDQRAF
                     EFKALEQGRDAARAERARLDRELSEQRARAEKAERDLMSSTNSKAYEAAIREVDAAKK
                     HISDLETKILEQMEAAEKAEAGIAEHEPQVARLRAEHEERLKAFEEQTRAQTAEIERR
                     RQERERLFTGLPPAAMSVYKRISTRIRDGIAVAEARNNSCSACAIKIRPHMMAEIRRG
                     EEIILCDNCSRILYYVPAEQPHSTSTVSAS"
BASE COUNT         1063 a         2175 c         2416 g         1112 t
ORIGIN      
        1 cgtgacgggg ccgctcgaac tgatgggcgc tcatgtgaaa actgaagacg gtcacgcgcc
       61 gctcgtcgtc gaggggcgta gcccgctccg cgccatcgag tacgagacgc cggtcgccag
      121 cgcgcaggtc aagtcgtgcg tcctgctcgc gggactcttc gccgagggcc ggacgagcgt
      181 cacggagccg cacgcgctga cgcgcaacca caccgagcgc atgctccgct ggttcggcgc
      241 gcacgtcgag accggccatg cggtgcgcgc cgacggctcg accgcgcacg tccattcggt
      301 cgaaggcgtg cagggcttcg gcgcgcgcga catcgacgtg ccgggcgaca tctcttcagc
      361 ggccttcatg gcctcggcgg cggcgttgct gcccggctcc gaactcatca tcgaaggcgt
      421 cggcctcaac cccacgcgct cgcgcttcct cgacacgctc gtcatgctcg gcgccgacgc
      481 gcgcgtcgag gacgagcggg atgtgtgcaa tgagccggtc ggcacggtgc gcgtgcgcgg
      541 cgcgcggggc gcggcggaag cactcgaccg cggcaagccc gcgctgctct tgcgcggcga
      601 gatgatcgcg cagctcatcg acgagctgcc cgtgctcgcc gtcgtcggca cgcaggtcga
      661 gggcggcatc gagatacgcg aggcgaagga gctgcgcgtc aaagagtccg accgcatccg
      721 cacgacggtc gataacctgc gcgcgatggg cgctcgtgta gaagagttcg aggacggctt
      781 gaaggtgcac ggtcgcgccc ggctcagagg cgcgcggctc gacgcgcacg gcgaccatcg
      841 catcgcgatg gccttcgccg tcgccgcgct cgtcgccgaa ggcgagaccg agatcgtcgg
      901 cgccgaagag tgcgtcgccg tctccttccc cgagttcttt ccgctgctcg aatcggtcac
      961 ggagcgttga gcttgaccac cacgccgcgc atcgtcatca cgggcttcat gggcgcgggc
     1021 aagacgacgg tcgccgccgc gatcgcgcgc ctgctcggct gcgccgccat cgacctcgac
     1081 gaccgcatca ccgcgcgcga ggggcgcacg ccgcagcagc tcatcgacga agacggcgag
     1141 gcggccttcc gcgacgtgga gacgcgcgcg ctcggagaag tgctcggcga ggaagcggcg
     1201 cgcgtcgtcg cgctcggcgg cggcgcgtgg gcgctcgaac gcaaccgcct cctcgtctgc
     1261 gagcacggct gcctcgccgt ctggctcgac gcgcccttcg agctgtgctg gcggcgcatc
     1321 atcgaagata gcggcgaaga ccggcccaac gcccgcgacc cctcgcgcgc ccgcgccctc
     1381 tacgacgcgc gccgccacgc ctactcgctc gccgcgctgc gcgtctgcgt cacccccgaa
     1441 cggagcgccg acgacatcgc cgccgagatt ctctccaccc cgcgcgcgtg aagcgggcag
     1501 cccgccgacg cccctgccgc gtctcaacga aacatttgcg cgccgcggaa cgaagagagt
     1561 acattccacg aaaacgacag aggggacatc atgggagaca gggacacgga cgcgaaaggc
     1621 tcgaagcccg cgaaggaact ccggaacgcg cacaactgga aacacctcgc ccgccggcac
     1681 ggcctcggct actacgaact acagaccgac gacacgggcg acgtgcccgt gcgtctgttc
     1741 ctcacgccgc agctcttgag cgacgccgag gacatcctct accgccagat cgtcaacgcc
     1801 acgcgcttcc ccggcgtgcg gatggtcgtc atcacgcccg acacgcacta cggctacggc
     1861 gtccccgtcg gctgcgtcct catcaccgac gccgacgcgg gcgccgtcgc gatggggccg
     1921 gtcggctacg acatcggttg cgggatgatg tcggcgcggt cgggtgtgga ggccgagcgg
     1981 gcgacgccgg agaagcgtct ggagttcaac cgggcggtga tggggcgggt ggatatgggg
     2041 ttcgggggga agagtaagcg gttgggcagg ctgtcggagg atgagttcca gaatctggtg
     2101 cggggcgggg cggagtatta cgtggagaag tacggggcga cgttcgatag gagtcgtgcg
     2161 gagcgtcatc gcatcccggt cgatgatgat tggcagattc cgtggggcgg gaaggggagg
     2221 ccggagcggg ggctggagca gatcggcagc ctcggcgggg gcaaccactt catcgagttg
     2281 cagcgctccg agcagacggg cacgctcttc ctacaggtgc acacggggag ccgcgggttc
     2341 gggcacgggc tggcgacgaa ctacttcgag ctggcgcgcg aggagcggcc cgagggggtg
     2401 aaggacatcg acctcgggta cttcacgccg gagtcgaggc actaccgcga ttaccagaac
     2461 gcggtcgcgg cgggcggcaa ctacgcgatc ctgaaccggc tcgtcatctt cgagcaggtg
     2521 tccgaggctt tccgcgaggt gttcggcgag gagttggagt tgatctacga gatcagccac
     2581 aacctcgtgc agcgcgagca gcacccggag ttcggcgacg tgtgggtgca ccgcaagggc
     2641 gcgacgcgcg ccttccccgc agggcacccg gcgctcgcgg ggacggtgtg ggaagggacg
     2701 ggccaccccg tcctgattcc ggggagcaac caggactgga gctacatcct gcgcccgctc
     2761 gaaggcgccg tccggagctg ctactccgtg taccacggcg cgggcagccg gatgtcgcgc
     2821 ggcgaggcgg cgcgcacgct ctcgcagcgc gcggtggacg acgagtacag ggaggcgggc
     2881 gtgctcgtca acgcggacgg gcgcgtgccg attgacgagg cggcggcgtg ctacaagcct
     2941 gcggaggaag tgatcgaggc ggtggtcggc gcaggcctcg ccgaggtcga gcaccgcttg
     3001 tggccgctcg cgtcgctgaa gggcgtggac gaggcgaaga accggaagcg gcgcaagggg
     3061 aaggggggcg tgctggggaa gaagtcgggc gagcattatt gagcgggcgc gattcccgtc
     3121 cggggccgcg ttcgtcactt cgaagagaca cgaaaggaag tcgaactatg agcaagagaa
     3181 ctctgttcgt gtgcggcgtg gccctcggcc ttgcgctctc cgcgctcacg ctcacggcag
     3241 cggagcaggc gggcgtgtcg ggcgccgccg agaggaggga ggtcgagcag cgggtgacgg
     3301 gcggcgtcgt cacgctgtac gcgctcgacc cgctcgcgcg cacgctctgc ttcgcggacg
     3361 ggcgtgacgg ccacgtcttt catgagaacg aggtcaggaa tcgttgcagc gacctcgact
     3421 tcggcagcta cagggcgggc cacttcagtg tcggcgtcga gggcgcgcgc ctgggcgccg
     3481 tcgtcaacct cggcaccgcg gacgaactcc ggcagcggta cggctacccg gagacggtcg
     3541 gcaacggcca gggcttcgcc tcgttgcggg ccgagggtgg gagggtcttg gtgctcaagg
     3601 gcgtgaggcc gcacgcggaa caggaactca aagagtcggc gctcctcttc ggcgaaggcg
     3661 cgggcgccgc gagcgcgccc gtcaagctcg gcaacatcta cctcgtccgc ctcaccgacc
     3721 ggcgcgacag ggatttcgaa agggtcgtga agctgaaggt catcgcgcac acgccgaacg
     3781 agtcggtcac ggtccgctgg caggcactct gacgcggccc gcaaaacggg agcgcgccac
     3841 tcaacgcgcg ccgtcgccct gcgccgcgcc gttgccgttc ttgcgcgcgc gcagaagttt
     3901 gagcagcgcc agctcctcca cctcgtagtc gggctggctg cgcgtgacgc ggcggagctt
     3961 gcgcgggcgg aactcttcga gcgtctgccc cttcacgtag cggtcgagca ccgccgggtg
     4021 gatgtacgag ccgcggcaga ccgtgggcgt gttgccgagg tgttcggcca cgcgcgcgac
     4081 ggcgcgcgcg acgttcttcc tggcctccct ccggtcttcg cagcacccga tttcggcgag
     4141 cgcgatggcc gccatcagcg tcccgcccca cgtgcggaag tccttcgccg aaaactccgg
     4201 cccggtcgcg cccttgatgt aatcgttcac gtcgcgcggc gtgacgggcc gcgccttgcc
     4261 gtcctcgccg aggtagttga agagctttgc gccgccgatt gacttgatgt cgcgcatcag
     4321 cgcggccagc tcttcgtcca ccatcacgcg gcggtgcttg acccggtgct tgccgacgaa
     4381 gtcgaagcag aggcggcccc ccggctggat gtcgaggtgg cggttgcgca gcgtcgtcac
     4441 gccgtaggtg cgataacgcc ggacgctctc ctcggagccg acgcggaagt agaggtcgtt
     4501 gatgaggcgg acgacgacgg ccagcacgcg ctccttcgtc aacccttcgc gcacgatgtc
     4561 ctcgttcgca cggcgcctga gcgcgggcag cgcctcgccg aagcgctcga ccttttcgaa
     4621 cttgtggcgc tggcggcgcg cgacggtcgt cgggtggtag atgcgttgcg cgcgcccctt
     4681 cgcgtcaacg ccgatggctt gcagggagtt gcgcggcgag ggcgagattc tgacctcgcg
     4741 ccacgcgggc gggatggcga gcgcgcggat gcgttcgagc gccgcctctt cgcggacgcg
     4801 cgcaccgtcg gcggtctcgt aacgaaagcc gctgcgcttg gtgccgcgcc tgcgccacca
     4861 gttcgcgcgg ctcccgcgtg taacacttcc gaccaccgcc gcgggcggcg tgttccgttg
     4921 aattgcaatc tccgcagtcg ccaacgcttt aatccccctg ttcatccgag gagcgttagc
     4981 gtgtgcaaac ttggggccgc ccggacagaa gagtgggaaa aggggaagaa ggggagggga
     5041 aaaggttaaa aggggagaag gcgaaaaggg aagaaagcga gtcgcttctt ctcccttttc
     5101 gccttttccc ctcttcccct tttcgccttt aaaccttccg ttttacctgc cgtggattac
     5161 gttgccgagt atctgcacga cgccgttctc gttgacgacg accgagagtt tgagttgccg
     5221 ccgatagctg agcggcgtcg ggaagccgat ggtcaggttc tcgatgcccg ccccgctctg
     5281 caacaagacc aagccgtacg ggttctgctg cgtgaagccg acgttgcgcg ccgccgcgaa
     5341 cgagatgttg gtcacgttcc gaccgtcgat gtcgaggatg acgaaggtca ggtctgtgtc
     5401 gccgccctgc ttggacacat gcgcgccgag gaacacgccg gggccttcga ggttgacgag
     5461 gacttgcggg gcggcacctt tcggcgccgg tgccttcagc ccttgcgtcc tctgcgggtc
     5521 gcacactccg ggggcgcagg cttgggcggt ctgcgcgctg ggtgtggcgg tggccgacgc
     5581 cttcccggat gacttcttgc tcgccccctt ggtcgccttt ctggttgcca ttttcgcacc
     5641 tcctcaaagt ccgaatcgaa aaagccggaa gggagtaagg atgaccgtgc ggcttctctg
     5701 tacgagagga agtttgcgcg ccgggggcgc gcggggcaag ggttgagcct tggcgaaagt
     5761 ctgctaactc gccgagacgg tcgaagtgct gtgcggctgc tcggcgggga cgtagtagag
     5821 gatgcggcta cagttgtcgc agaggatgat ctcttcgccg cggcgaatct cggccatcat
     5881 gtgcgggcgg atcttgatgg cgcaggcgga gcaggagttg ttgcgcgcct cggccacggc
     5941 gatgccgtcg cggatgcgcg tgctgatgcg cttgtagacg gacatggcgg cgggcggcag
     6001 gcccgtgaac aagcgttcgc gctcctgccg gcggcgttcg atctcggcgg tctgcgcgcg
     6061 ggtctgctct tcgaaagcct tgagccgctc ctcgtgctcg gcgcgcaggc gcgcgacctg
     6121 cggctcgtgc tcggcgatgc cggcctcggc cttctcggcg gcctccatct gttcgaggat
     6181 tttcgtctcc agatcggaga tgtgtttctt ggcggcgtcc acctcgcgga tggcggcctc
     6241 gtaggctttc gagttggtcg aggacatcag gtcgcgctcg gccttctcgg ctcgggcgcg
     6301 ctgctccgag agttcgcggt cgagccgcgc ccgctcggcg cgggccgcgt cgcgcccctg
     6361 ttcgagcgcc ttaaattcaa aggcgcgctg gtcgaattct gcttcgatct cagcgcgcct
     6421 tcgtgggatg gcgtccagtt cggcctgtag tctgcggatg gccgtatccg tctcctgtaa
     6481 agcgatcaac tgctccaatt ccgctttcac tctcttcaag tctcctcgaa atatcagcac
     6541 gggcgccacg gcacgcgcgg gggttgatgg atgattctct cagaagaggt ggcgggtgac
     6601 aagcgacgag aataagggct gaggactgag gactgaggac tgattgaaag gagtgaagcg
     6661 tagaccgcga gccgtgatga gccgcggaca agttttaact cagtcctcag tcctcagtcc
     6721 tcagcactat gttgccgaac gtctcgttcg attcgagccg ggtgtg
//
LOCUS       DBNGOH010000487         5028 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3371806, whole genome shotgun sequence.
ACCESSION   DBNGOH010000487 DBNGOH010000000
VERSION     DBNGOH010000487.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5028)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5028)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5028
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3371806"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..357
                     /locus_tag="AC159P_09630"
     CDS             <1..357
                     /locus_tag="AC159P_09630"
                     /inference="COORDINATES: protein motif:HMM:NF019229.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASPIC/UnbV domain-containing protein"
                     /protein_id="HIR3695752.1"
                     /translation="GDLDNDGDTDIVLAQTDGPPVVLRNEGTKNHWFGLALAGARGNR
                     HGLGARVTVTDAAGARQIFDVSAAGSYLSSSDPRLIVGLGNKTGVRSVEVRWPGGKTQ
                     TVNDPQIDKYLTISER"
     gene            424..2295
                     /locus_tag="AC159P_09635"
     CDS             424..2295
                     /locus_tag="AC159P_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020713094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HIR3695753.1"
                     /translation="MLFQRPPRRTLGAVALAFSASLLIALSSATVVAQPNTPQPQPTQ
                     QSQSMGVSTGSAVVSVTKRTVGVVDPKAPKVFEDVTARTALAAFRHRSGGAAKDYILE
                     TVSGGVAIFDYDNDGLADIYLLNGSTFAAERGREKPPRAALYRNLGNWKFEDVTEKAG
                     VGNERWGMGVAVGDYDNDGHADLFVGNYGTSRLYRNNGDGTFTDVAPRVGVARKGWST
                     GASFGDYDRDGRLDLFVPGYVEFDSNKLPPSPSDVPKPGEIAQNFCQFRGVAVMCGPR
                     GLKGEGDTLYRQKADGTFEDVSAKTGAGDETRYYGFSSVWVRVNEDDLVDLLVINDST
                     PNQLYMNLGGGKFEEVGYPSGVALNENGREQAGMGLGVGDYDNDGLVDFYVTNFSDDS
                     NTLYHNDGEGTFTDVTFQSGHGEPTIPFLGWGASFVDYDNDGWKDVIVANGHVYPAVD
                     KQQWGTSYAQQLLLFRNTTSNGRFERVGAAPGSGLAEAWTTRGLAVGDLDNDGRADLV
                     LNNIDSPPTVLRNVSDAKHHWLRLRLVGDPSKRSPRDATGATVYVTTGKLRQRGDVVS
                     GAGYSSQNDPALSFGLGPATKVDALLVKWPDGTAETFDVPGIDRTLTLTQGKGAAAK"
     gene            complement(2321..2761)
                     /locus_tag="AC159P_09640"
     CDS             complement(2321..2761)
                     /locus_tag="AC159P_09640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695754.1"
                     /translation="MKLSRVMLAVCFVVACATVSRAQGEGGQPPPASVGTSAPPRVLR
                     TRASRAAVVRLKNGESLACHFLGADDDTIEVEASGARRQIALDAVAGIDFSPRAPSEG
                     KTQTAARGATVAAAAQTPLVVTARLAERPGTLPPRPLSVSCLLF"
     gene            complement(2951..3556)
                     /locus_tag="AC159P_09645"
     CDS             complement(2951..3556)
                     /locus_tag="AC159P_09645"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015314846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HIR3695755.1"
                     /translation="MNNNGVARFDLFTARDFFDPHTCARLVAEMRAAQGGPASVYGSS
                     EAGAVEERVRRVTRVAASRETVGLVTRRLAECRMEVERHFGASLGECEEPQFLRYRVG
                     DFFVAHQDGNTPLLRLERERERRVSVVIFLNRQSEAPAPDAYGGGWLAFSAPRAEQLR
                     LAPDAGTLVAFRSETTHEVTPVTHGERCSIVSWYWRKSREP"
     gene            complement(3595..4797)
                     /locus_tag="AC159P_09650"
     CDS             complement(3595..4797)
                     /locus_tag="AC159P_09650"
                     /inference="COORDINATES: protein motif:HMM:NF012692.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HIR3695756.1"
                     /translation="MRRIDLTKAQAARSSTIRDINRQIVLNYIRDREPISRAEIARVT
                     EMQRSTVSNIVEGLKSDGLIEEIGAGVSTGGRRPTLLRLRAAGAFAIGIDITPSSTTV
                     ATSDLVGRVLEREDVPNSTRPDEMAARIIECVSDFADRDRYATIAGVGVSLPGMVDPD
                     TGRAIYIPYFKWRDWAIAEEIERATGLKAVVDNDANAAALAELWFGRPEVSDSRDFIM
                     ALVAEGIGTGIVIDGQIYRGERGAAGEFGHMIVGPHAPVACSCGNLDCWEAFSSERAA
                     VARYLRNAGLEDDGADSARACFREVVERALGGEQAAIDALTETAHYLGIGISNLIVGL
                     SPAAVVVSGHITRAWPLIAPSLEETIQRSIRRGLPSARIVASTLSEPTLLGAISLVLS
                     RKFGLAEV"
BASE COUNT          827 a         1727 c         1700 g          774 t
ORIGIN      
        1 ggcgacctcg acaacgacgg cgacacggac atcgtcctcg cgcagacgga cggcccgccc
       61 gtcgtgctcc gcaacgaagg cacgaagaac cactggttcg gcctcgcgct cgcgggcgcg
      121 cgcggcaacc gccacggcct cggcgcgcgc gtcacggtca cggacgccgc gggcgcgcga
      181 cagatttttg acgtgtccgc cgccggcagt tatctttctt cgagcgaccc gcgattgatc
      241 gtcggcctcg gcaacaagac cggcgtccgc tccgtcgaag tccgctggcc cggcggcaag
      301 acgcagaccg tcaacgaccc gcagatcgat aagtacctga ccatcagcga aagatgagtt
      361 gcgaccgtca acgttagacc gttaatgaat gataagtccg agcgtcaacg gaagatgagt
      421 ttcatgctgt ttcaacgtcc cccccgccgc acgctgggtg cagtcgcact agctttctcc
      481 gcgtcgctcc tcatcgcgct ctcgtccgcg accgtcgtcg cgcagcctaa caccccgcag
      541 ccgcagccga cgcagcagtc gcagagcatg ggcgtctcga cgggcagcgc cgtggtctcc
      601 gtgacgaagc ggacggtcgg cgtcgttgac ccgaaggcgc cgaaggtctt cgaggacgtg
      661 acggcgcgca ccgcgctcgc cgcgttccgg catcgttcgg gcggcgcggc gaaggactac
      721 atcttggaga ccgtgtcggg cggcgtcgcc attttcgatt acgacaacga cggcctcgcg
      781 gacatctacc ttttgaacgg ctcgaccttc gcggcggagc ggggccgcga gaagccgccg
      841 cgcgcggcgc tctaccgcaa cctcggcaac tggaagttcg aggacgtgac ggagaaggcg
      901 ggcgtcggga acgagcgctg ggggatgggc gtcgccgtcg gcgactacga caacgacggc
      961 cacgccgacc tcttcgtcgg caactacggc acgtcgcgcc tctaccgcaa caacggcgac
     1021 ggcaccttca cggacgtggc gccgcgggtc ggcgtcgcgc gcaagggctg gtcaacgggc
     1081 gcgtcgttcg gcgactacga ccgcgacggg cggctcgacc tgttcgtccc cggctacgtc
     1141 gagttcgatt cgaacaagct gccgcccagc ccttcggacg tgccgaagcc cggcgagatc
     1201 gcgcagaact tctgccagtt ccgcggcgtc gccgtgatgt gcggcccgcg cgggctgaag
     1261 ggcgagggcg acacgctcta ccggcagaag gcggacggca ccttcgagga cgtgtcggcg
     1321 aagacaggcg cgggcgacga gacgcgctac tacggcttct cttcggtctg ggtgcgcgtc
     1381 aacgaagacg acctcgttga cctgctcgtc atcaacgact cgacgccgaa ccagctctac
     1441 atgaacctcg gcggcggcaa gttcgaggag gtcgggtatc cttcgggcgt ggcgctcaac
     1501 gagaacgggc gcgagcaggc ggggatgggc ctcggcgtcg gtgactacga caacgacggc
     1561 ctcgtcgatt tctacgtcac caacttctcc gacgactcga acacgctcta ccacaacgac
     1621 ggcgaaggca ccttcacgga cgtgactttc cagtcgggcc acggcgagcc gacgatcccc
     1681 ttcctcggct ggggcgcgag cttcgtcgat tacgacaacg acggctggaa ggatgtcatc
     1741 gtcgccaacg gccacgtcta ccccgcggtc gataagcagc agtggggcac ttcctacgcg
     1801 cagcagttgc ttctgttccg caacacgacc tcgaacggga ggttcgagcg cgtcggcgcg
     1861 gcgcccggca gcggactcgc cgaggcgtgg acgacccgcg gcctcgccgt cggcgacctc
     1921 gacaacgacg ggcgcgcaga cctcgtcctc aacaacatcg actcgccgcc gaccgtcctg
     1981 cgcaacgtct cggacgccaa gcaccactgg ctccgcctgc gcctcgtcgg cgacccctcg
     2041 aagcgcagcc cgcgcgacgc cacgggcgcc accgtctacg tcaccaccgg caagctccgg
     2101 cagcgcggcg acgtggtgag cggcgccggc tactcctcgc agaacgaccc cgccctctcc
     2161 ttcggcctcg gccccgcgac gaaggttgac gccctcctcg tcaagtggcc cgacggcacc
     2221 gccgaaacct tcgacgtgcc cggcatcgac cgcacactca ccctcacgca aggcaaaggc
     2281 gccgcagcga agtaggcagt aggcggtagg cggtaggcag ttaaaacaga agacaggaga
     2341 cagaaagagg cctgggaggt aaagtcccgg gcctctccgc cagacgggcg gtcacgacga
     2401 gcggcgtctg ggccgcggcg gcgacggtgg ccccgcgcgc cgccgtttgt gtcttgccct
     2461 ccgacggcgc gcgcggcgag aagtcgatgc ccgccaccgc gtcgagcgcg atctggcgcc
     2521 gcgcgcccga cgcctcgacc tcgatggtgt cgtcgtccgc gccgaggaag tggcaggcga
     2581 gcgactcgcc gttcttcagc ctgacgacgg cggcgcggct cgcgcgcgtc cgcaaaactc
     2641 tgggcggcgc gctcgtcccc accgacgccg gaggcggctg cccgccctca ccctgcgcgc
     2701 gcgagacggt cgcgcacgcc acaacaaagc acacggccaa catgacgcgg ctcaacttca
     2761 tggctgcttc ctttccttct gctcggctgt tttcgggagc ggctgctccg ggggcaggag
     2821 atgcgggagg tctcaaccac gggctaacaa attacgtcgc cgccacacaa cagaattttc
     2881 taaaagccgt gaaccgtaaa ccgtaaaccg tgataagaga aacctcatcc ctcttatcac
     2941 ggtttacggt ttacggttca cggcttttcc tccagtacca actgacaatc gagcagcgct
     3001 cgccgtgcgt cacgggcgtc acctcgtggg tggtctcgga gcggaaggcg acgagggtgc
     3061 cggcgtcggg ggcgagccgg agctgttcgg cgcgcggcgc ggagaaggcg agccagccgc
     3121 cgccgtaggc gtcgggcgcg ggggcctccg actgtctgtt caggaagatg acgacggaga
     3181 cgcggcgctc tcgctcgcgt tcgagccgca gcagcggcgt gttgccgtcc tgatgcgcga
     3241 cgaagaaatc gcccacgcga tagcgtagga actgcggctc ctcgcactcg ccgaggctcg
     3301 cgccgaaatg ccgctcgact tccatcctgc actccgccag ccgccgcgtc accagcccga
     3361 ccgtctcgcg cgatgcggcg acgcgcgtca cgcggcgcac gcgctcctcg accgcgcccg
     3421 cctcgctgct gccgtagacg ctcgccgggc cgccctgcgc ggcgcgcatc tccgcgacga
     3481 gacgcgcgca cgtatgcggg tcgaagaagt ctctcgccgt gaacaggtcg aagcgcgcca
     3541 ccccgttgtt gttcatcgct cgtctatttg tctgtgcgtc tctacgcctg ttcgttagac
     3601 ctcggccagt ccgaacttcc ttgagaggac gaggctgatg gcgccgagca gggtcggctc
     3661 gctgagggtc gaggcgacga tgcgtgcgga ggggaggccg cggcggatgc tgcgctggat
     3721 ggtctcttcg agcgaggggg cgatgagcgg ccacgcgcgc gtgatgtggc cgctgacgac
     3781 gaccgccgcg gggctgaggc cgacgatcag attcgagatg ccgatgccga ggtagtgcgc
     3841 cgtctccgtg agcgcgtcga tggcggcctg ctcgccgccg agggcgcgct cgacgacctc
     3901 gcggaagcac gcgcgcgcgg agtccgcgcc gtcgtcttcg aggccggcgt tgcgcaggta
     3961 gcgggcgacc gccgcgcgct ccgaggagaa ggcttcccag cagtcgaggt tgccgcagga
     4021 gcaggcgacc ggcgcgtgcg gcccgacgat catgtggccg aactcgcccg ccgcgccgcg
     4081 ctcgccgcgg taaatctggc cgtcgatgac gatgccggtg ccgatgccct cggcgacgag
     4141 cgccatgatg aagtcgcgcg agtcgctgac ctcggggcgc ccgaaccaca gctcggccag
     4201 cgccgccgcg ttggcgtcgt tgtccaccac cgccttcaga ccggtcgcgc gctcgatctc
     4261 ttcggcgatg gcccagtcgc gccacttgaa gtaggggatg tagatggcgc ggcccgtgtc
     4321 cggatcgacc atgccgggca ggctcacgcc gacgcccgcg atggtcgcgt agcggtcgcg
     4381 gtcggcgaag tcgctgacgc actcgatgat gcgcgcggcc atctcgtcgg ggcgcgtgct
     4441 gttcggcacg tcctcgcgtt cgaggacgcg cccgaccaga tcgctcgtcg ccacggtggt
     4501 cgaagacggc gtgatgtcga tgccgatggc aaacgcgccc gccgcgcgca ggcgcagcag
     4561 cgtgggccgc cgcccgccgg tcgagacgcc cgcgccgatc tcctcgatca gcccgtccga
     4621 cttcagcccc tcgacgatgt tcgagaccgt cgagcgctgc atctcggtga cgcgcgcgat
     4681 ctcggcgcgc gagatcggct cgcggtcgcg gatgtagttg agaactatct ggcggttgat
     4741 gtcgcggatc gtcgaggagc gcgcggcctg cgccttggtg agatcgattc ttcgcatcgg
     4801 gtgttcttag ggctgtcctt cgggccggtc gcccgccgcc gagtcgcgcg ctccggacgg
     4861 gcgacgctac ttgtattccg tccggggtct cgcggtcaac tattttgtga gaaggagaca
     4921 ggagtcagga gtcagaagaa agaagcgatg aaggagcttg agagctaaag tgacaagcat
     4981 ctttcgctga ctgccggacg agcagagtgc ccaggcactt tatctctc
//
LOCUS       DBNGOH010000488         2604 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1173213, whole genome shotgun sequence.
ACCESSION   DBNGOH010000488 DBNGOH010000000
VERSION     DBNGOH010000488.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2604)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2604)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2604
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1173213"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            330..1190
                     /gene="cdaA"
                     /locus_tag="AC159P_09655"
     CDS             330..1190
                     /gene="cdaA"
                     /locus_tag="AC159P_09655"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HIR3695757.1"
                     /translation="MFQPSYNHYLDRLREPHSYVDIFLVFLMVYALLKLIRGTRAAPM
                     AAGIGGLALLYWVAANYDLGTLEFVLRPAPIYIGFAIIILFQNEIRQALITFGNRIRI
                     PLLLRHRGQFGEGVYDEIVLAATTLASTKTGALVVIERAASLNNVVEGGVKLDAELSY
                     DLLVTIFNPATPLHDGAVVIRGHRAAAAACFLPLTLNPMLSKDLGTRHRAAIGITEDT
                     DAVAVVVSEETGIISFVQGGHIRRNLDATKLRAAILEALEPPSRRDEEEQAEADNAEV
                     RGQSLEVSKN"
     gene            1294..2259
                     /locus_tag="AC159P_09660"
     CDS             1294..2259
                     /locus_tag="AC159P_09660"
                     /inference="COORDINATES: protein motif:HMM:NF019561.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HIR3695758.1"
                     /translation="MAFREAKRKGTKGDARRVVAEWLREIFLEDLGLKLLALVIAVGL
                     WFAVTSLRAPATVRLRGVPLEFILPDNVEISNEPADEVDVTLEGSQGRLSEVNARNLV
                     ARADITQLRPGERNARLSEKNVTMDLPNGVRIVNIEPRSVLLRLETIIEHEVDIEARF
                     EGQPPEGYVLGNVQITPARARVRGPESHVRAVDKAHTETIPLDGLRETFVTQTAVDIA
                     DRKVAPLDAVVTVRVEIAEERTEKRLADVSVVSAVGGQPQPATAAVTLRGPRSALAAL
                     RPQDIRITLEQTPDGALRPRLSLPPNLEGRVELVSTAPAEFNVNR"
     gene            2354..>2604
                     /locus_tag="AC159P_09665"
     CDS             2354..>2604
                     /locus_tag="AC159P_09665"
                     /inference="COORDINATES: protein motif:HMM:NF014877.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HIR3695759.1"
                     /translation="MKKLFGTDGMRGEAGRFPLDAATVRTAGRSLARHLTAKTGRAPR
                     IVAGRDTRESGAWIERAFLDGALEAGAACESAGVITTPGV"
BASE COUNT          479 a          935 c          789 g          401 t
ORIGIN      
        1 tcctttcaac gtgcgacccg gcggcggatg atagcacagc gaatcggagc gaggctcccg
       61 ttgatttatg ccgttttttg cagcataatc ggcgcggagg cgtgtaggct caacccgctg
      121 tgggtgactg ttgatgcctg cgtttctcgg cttgccgcgc ggcaaatagc agagagacgc
      181 gccgggaagc cgacaagccg gagcgcagaa tcttcgattt ccgattttgg attttcgatt
      241 ggaagcggcc atgcaactcg tacccgagac cgaaggcttc aatctagaac tcgaatccga
      301 aatccaaaga ccacagtccg aagtcgatca tgtttcagcc gtcgtacaac cactacctgg
      361 acagactccg cgagccgcac agctacgtgg acatcttcct cgtgttcctg atggtgtacg
      421 cgctgctgaa gctgatccgc ggcacgcgcg ccgcgccgat ggccgccggc atcggcggac
      481 tcgcgctgct ctactgggtc gcggccaact acgacctggg gacgcttgag ttcgtcctgc
      541 gtccggcgcc gatctacatc ggcttcgcca tcatcatcct cttccagaac gaaatccgtc
      601 aggcgctcat caccttcggc aaccgcatcc gcatccccct gctcttgcgc caccgcgggc
      661 agttcggcga gggcgtctac gacgagatcg tgctcgccgc gaccacgctc gcctcgacca
      721 agacgggcgc gctcgtcgtc atcgaacgcg cggcgagcct gaacaacgtc gtcgagggcg
      781 gcgtcaagct cgacgccgag ctgtcctacg acctgctcgt gacgatcttc aacccggcca
      841 ccccgctgca cgacggcgcg gtcgtcatcc gcggccaccg cgctgccgcc gccgcgtgct
      901 tcctgccgct caccctgaac ccgatgctct cgaaagacct cggcacgcgc caccgcgccg
      961 ccatcggcat caccgaggac acggacgccg tcgccgtcgt cgtctccgaa gagaccggca
     1021 tcatctcctt cgtgcagggc ggccacatcc gccgcaacct cgacgcgacg aagctgcgcg
     1081 ccgccatcct cgaagccctc gaaccgccct cgcgccgcga cgaagaagag caggccgagg
     1141 ccgacaacgc ggaggtcaga ggacagagcc tggaggtcag taaaaactga cccttactga
     1201 ctttcgtcac gcgacctttc atcaaccaaa cacggatcgt caaagcgact acggacttcc
     1261 gacttataat ttccgacttc cgacctctga cgcatggcat ttcgtgaagc gaaaaggaaa
     1321 ggcacgaagg gcgacgcgcg gcgcgtggtc gccgagtggc tgcgcgagat tttcctcgaa
     1381 gacctcgggc tgaaactcct cgcgctcgtc atcgcggtcg gcctgtggtt cgccgtaacg
     1441 agcctgcgcg cgcccgccac cgtccgcctg cgcggcgtcc cgctcgaatt catcctgccc
     1501 gacaacgtcg agatcagcaa cgaacccgcc gacgaagtgg acgtgacgct cgaaggcagt
     1561 cagggccggc tctccgaagt caacgcgcgc aacctcgtcg cccgcgcaga catcacgcag
     1621 ctccgccccg gcgagcgcaa cgcgcggctg tccgagaaga acgtcacgat ggacttgccc
     1681 aacggcgtcc gcatcgtcaa catcgagccg cgcagcgtgc tgctccgcct ggaaacaatc
     1741 atcgagcacg aggtggacat cgaggcgcgg ttcgagggcc agccgcccga aggctacgtg
     1801 ctcggcaacg tgcagatcac gcccgcgcgc gcccgcgtcc gcggccccga aagccacgtg
     1861 cgcgccgtgg acaaagcaca caccgagacc atcccgctcg acggcctgcg cgagacgttc
     1921 gtcacgcaga ccgccgtgga catcgccgac cgcaaggtcg cgccgctcga cgcggtcgtc
     1981 accgtgcgcg tcgagatcgc cgaagagcgc accgaaaaac gtctggccga cgtgtcggtc
     2041 gtctccgccg tcggcggcca gccgcagccc gcgaccgccg ccgtcacgct gcgcggcccg
     2101 cgctcggccc tcgccgcgct gcgcccgcag gacatccgca tcacgctcga acagacgccc
     2161 gacggcgcgc tccgcccgcg cctctcccta ccaccgaacc tcgaaggccg cgtcgaactc
     2221 gtctcgaccg cccccgcgga gttcaacgtc aacagatgaa tgttcatagt gcggagttcg
     2281 aagttcaagg cttcaagttc aacgccggag agttgtccgt gacctcgaac catgaacccc
     2341 gacgattgcg acgatgaaaa aactattcgg cactgacggc atgcgcggcg aggcggggcg
     2401 cttcccgctc gacgcggcga ccgtgcgcac ggcggggcgc tcgctcgcca gacacttgac
     2461 ggcgaagacc ggtcgcgcgc cgcgcatcgt cgcggggcgc gacacgcgcg agtccggcgc
     2521 ttggatcgag cgggcgttcc tcgacggcgc cctcgaggcg ggcgccgcgt gcgagtcggc
     2581 gggcgtcatc acgacgccgg gcgt
//
LOCUS       DBNGOH010000489         7131 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2087645, whole genome shotgun sequence.
ACCESSION   DBNGOH010000489 DBNGOH010000000
VERSION     DBNGOH010000489.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7131)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7131)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7131
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2087645"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            140..898
                     /locus_tag="AC159P_09670"
     CDS             140..898
                     /locus_tag="AC159P_09670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695760.1"
                     /translation="MTTTSSQPLEFDAQSLAVWPVLAEWRDGKGIRRVESTRPFDVTQ
                     QGASFRLSRPPEIGQLMHLTLPVAMTRGQRRGAGIDAGFLSLVWAVSEPLPEEESDAA
                     PPRSVSVLFLGQASSTAFAAAEGAYAYGIDEGGLFRLQPRAAETSAAVARHTDMRRAT
                     RLDLPVEVTLEAFDRHGNVTMREQTVTENISRKGALVPTTLLIPPDSTVRLTNAERRI
                     SLNAVVRARRVGQNGVARLHLHIPEDEWPLDGLH"
     gene            complement(1068..1748)
                     /locus_tag="AC159P_09675"
     CDS             complement(1068..1748)
                     /locus_tag="AC159P_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR3695761.1"
                     /translation="MRKVLIVEDDQAMAVALRDGFTYEGYTVQVARDGSAGLRLAQEK
                     GLDLVILDVMLPRMSGLDVCRQLRGEGNQTPIIMLTARGQEIDKVLGLKTGADDYVTK
                     PFSFLELMARVEAVLRRAAKPSEQVETVEFGDVAVNFKTFEAAKGGRAIELSPREFKM
                     MKYFVEHRGEVVSRDQLLDHVWGYDGLPLTRTVDMHIAKLRQKIEDTPSDPHHIITVH
                     RVGYKFTG"
     gene            complement(1812..3524)
                     /locus_tag="AC159P_09680"
     CDS             complement(1812..3524)
                     /locus_tag="AC159P_09680"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3695762.1"
                     /translation="MTKLRTFFKRHILWVGLAAVVVPLLSIIALQYWSLLKLEKTSIV
                     AGHVGMKNYLADVSKEVKMFYTTNAEQSLNVSAASIQGDLLKTKYHFSKCKVEGAKRL
                     FISSFGPGGEPQTVFYSPYDQTRLGDVPPGEARAAQIATVPLRVLAKDGTLVKTSKLY
                     VDDRDHENRVLFKPVVDDWGKVVGATGMILDSDYFKAQLMPRLIRESLPKFFPDGADE
                     NVIVSLYDRPGNQVFASQDAKGQNDVSWVNPSYFYDYTLKIRSRNMTPEQWAHWNFNF
                     SLSLSLLMTGVLISGIVMALRTASREMRLSQMKTDFVSNVSHELRTPLSSIRVFGEFM
                     KLGRVRDDEKIREYGEHIETESRRLTQLINNILDFSKIESGRKTYRFERVSIEDVVAE
                     TLKTCEVRLKQSDFTIVFECSPVSLPPVVIDRDAIAQALMNLMDNAVKYSEQAEKKEV
                     VVAVGRREDFVVVSVTDHGVGIAREEQKKIFEKFYRVSTGLVHDVKGSGLGLSLVKHI
                     VEAHHGRVTVESEPGRGSTFTIHLPAEHAADHSEAGDTRRQEERRTKPLSEPGTSFGF
                     GIKH"
     gene            3730..3987
                     /locus_tag="AC159P_09685"
     CDS             3730..3987
                     /locus_tag="AC159P_09685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695763.1"
                     /translation="MKHLRLLRLTCAAYLLLCAASFAYRAAAATVHDISAGSGHAQTA
                     TPRPCLLLHGKRLFTLAGYEVNASFTPGGKFHSFELVSSKR"
     gene            complement(4088..5044)
                     /locus_tag="AC159P_09690"
     CDS             complement(4088..5044)
                     /locus_tag="AC159P_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006241662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695764.1"
                     /translation="MKSPIRILLQTTIPETVDDWSIARFSMLREQLASLKDGAGKALC
                     EVTGRDRERDAEGNDPVLSRLDESEYDELWLFAVDAGDGLSEGDCAGITRFRGRGGGI
                     LSTRDHEDLGSCLCTIGGVGAAHFFHTKNPEPDESRRCVDDTATTDISWPNYHSGSNG
                     DYQEITLTEPPHELLRNPAAADGLIRLFPAHPHEGAVGVPRDAEGARVVATGKSKVTG
                     RPFNLVVAFERGANGDGDTAAGRAVAESSFHHFVDYNFDPHKGCPSFVAEPPGDGYEK
                     DPRALEDIKTYIRNLALWLAPEESSRQKAVGSRQQEQQSEGA"
     gene            complement(5163..6122)
                     /locus_tag="AC159P_09695"
     CDS             complement(5163..6122)
                     /locus_tag="AC159P_09695"
                     /EC_number="1.6.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - quinone reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone reductase"
                     /protein_id="HIR3695765.1"
                     /translation="MKAIRVHEFGGPEVLRLEEVADPQPSEGEVSVRVRAAGVNPVDT
                     YIRSGGHAIKPSLPYTPGLDAAGVVEAVGAGVTRVAAGDRVYTAGTVSGAYAELAVCQ
                     EAQVYRLPERVSFAQGAGVFTPYATAYRALFNRGQGRAGESVLVHGASGGVGTAAVQF
                     ARAAGFKVIGTGGTEEGRRLVAGQGAHHVLDHRAPDYLEQLKTLTGGEGVDIILEMLS
                     NVNLGKDLDLLAKGGRVVVVGSRGTVEINPRALMARDASIHGLTLFNATPRELEGVHA
                     AIGAGLENATLNPVVGRELPLADAARAHEVVLEPGAYGKIVLV"
     gene            complement(6345..7103)
                     /locus_tag="AC159P_09700"
     CDS             complement(6345..7103)
                     /locus_tag="AC159P_09700"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015120301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3695766.1"
                     /translation="MNGLEGKHVLITGASQGLGRQLALEFAREGAAALVISARTKDAL
                     EEVRAAIEEAAPGARVLAVAADLRRHEDVERLVADALAEFGGRLDVLVNNASIIGPVP
                     LPFLLDYPLEDFRAVLETNLVAPFLLIKKALPAMVEAGGSIINVTSDAGRTGYPGWGA
                     YGISKFGIEGLSETWAAELEGSGVRVNWVDPGEMNTKMHRDAEPEEDPTQWADPAEVT
                     EVFVFLASDESANVNGRRFRAQEDWRAEAAQQVS"
BASE COUNT         1137 a         2507 c         2238 g         1249 t
ORIGIN      
        1 cgtctaccag atcgccgaga tgggcttcgc actgcgcttc acgggctccg acgagacgga
       61 gcacaggatg ctcgcggcct gcctcgatta cctgcgcgag agcaaggccg agaccgccct
      121 ctgttaacga ggaatgatga tgacgaccac ctcttcacag ccgctcgaat tcgacgcgca
      181 gtccctcgcg gtctggcccg tcctggccga gtggcgcgac ggcaaaggca tccgccgcgt
      241 cgagtccacg cgcccgttcg acgtgacgca gcagggcgcc tcgttcaggc tctcgcgccc
      301 gcccgagatc ggccaactga tgcacctcac gctgcccgtc gccatgacgc gcgggcagcg
      361 ccggggcgcg ggcatcgacg cgggctttct gtcgctggtc tgggctgtct ccgagccgct
      421 gcccgaagaa gaatcggacg ccgccccgcc gcgctccgtc agcgtgctct tcctcggcca
      481 ggcttcctcg acggccttcg ccgcggcaga aggcgcctac gcctacggga tcgacgaagg
      541 cgggctgttc cgcctccagc cgcgggccgc ggagacgagc gccgccgtcg cgcgccacac
      601 cgacatgcgg cgcgcgacgc ggctcgacct gcccgtcgaa gtcaccctcg aagcgttcga
      661 ccgccacggc aacgtcacca tgcgcgagca gaccgtcacc gaaaacatca gccgcaaggg
      721 cgcgctcgtg ccgaccacgc tgctgatccc gcccgacagc acggtgcggc tgacgaacgc
      781 cgagcggcgg atttcgctca acgccgtcgt ccgcgcccgc cgcgtcggcc agaacggcgt
      841 cgcgcgcctc cacctgcaca tccccgaaga cgagtggccg ctcgacggac ttcactgagc
      901 cgccacgccc gtccccgtcc gtcgagtaca gcgcgcgttc ctctcaacac agaggacaca
      961 cagtgggcac ggagaaagac aattcttctc cgtgccctct gtgtgtcctc tgtgttgaag
     1021 ctcgcccgcg ccgacactca cgccggctag tcaccgtcac gaaagcccta gcccgtgaac
     1081 ttgtagccga cgcggtggac ggtgatgatg tggtgcgggt cgctcggcgt gtcctcgatc
     1141 ttctgccgca gcttggcgat gtgcatgtcc acggtgcgcg tgagcggcag cccgtcgtag
     1201 ccccagacgt ggtcgaggag ttggtcgcgg ctgacgacct cgccgcggtg ctcgacgaag
     1261 tacttcatca tcttgaactc gcgcgggctg agttcgatgg cgcgcccgcc cttcgccgcc
     1321 tcgaaggtct tgaagttgac cgccacgtcg ccgaactcga cggtctcgac ctgctccgac
     1381 ggcttcgccg cgcgccgcag cacggcctcg acgcgcgcca tcagctccag aaagctgaac
     1441 ggcttggtca cgtagtcgtc cgcgcccgtc ttgaggccca gcaccttgtc gatctcctgc
     1501 ccgcgcgccg tgagcatgat gatcggcgtc tggttgccct cgcccctgag ctgccggcac
     1561 acgtcgaggc cgctcatccg cggcagcatc acgtcgagga tgacgaggtc gaggcccttc
     1621 tcctgcgcca gccggaggcc cgccgacccg tcccgcgcca cctgcaccgt atagccctcg
     1681 tacgtgaacc cgtccctgag cgccacggcc atcgcctgat cgtcttcgac aattaagact
     1741 ttacgcatca taaataaaag gactgaggac tgaggactga gggccggttt tcaactcagt
     1801 cctcagtact tctaatgttt gattccgaag ccgaacgacg tgcccggctc ggagaggggt
     1861 ttggtgcggc gctcctcctg gcgccgcgtg tcgccggcct cggagtggtc ggcggcgtgc
     1921 tcggcgggca gatggatggt gaaggtgctg ccgcgccccg gctcgctctc gacggtgacg
     1981 cgcccgtggt gcgcctcgac gatgtgcttg accaaagaga ggccgaggcc gctgcccttc
     2041 acgtcgtgga cgaggcccgt cgagacgcgg tagaacttct cgaaaatctt cttctgctcc
     2101 tcgcgcgcga tgccgacgcc gtggtcggtc accgagacga cgacgaagtc ctcccgccgc
     2161 ccgacggcca ccaccacctc cttcttctcc gcctgctccg aatacttgac ggcgttatcc
     2221 atcaggttca tcagcgcctg cgcgatggcg tcgcggtcga tgacgacggg cggcaagctg
     2281 accggcgagc actcgaagac gatggtgaag tcgctctgct tgaggcgcac ctcgcaggtc
     2341 ttcaaggtct cggccaccac gtcttcgatg gagacgcgct cgaagcggta ggtcttgcgc
     2401 cccgactcga tcttcgagaa gtcgaggatg ttgttgatga gctgcgtcag gcgccggctc
     2461 tcggtctcga tgtgctcgcc gtattcgcgg atcttctcgt cgtcgcgcac gcgcccgagc
     2521 ttcatgaact cgccgaagac gcggatcgag gagagcgggg tgcgcagctc gtgcgagacg
     2581 ttcgagacga agtcggtctt catctgcgac aggcgcatct cgcgcgaggc cgtgcgcagc
     2641 gccatcacga tgccgctgat gagcacgccc gtcatcagca gcgagagcga gagcgagaag
     2701 ttgaagttcc agtgcgccca ctgctcgggc gtcatgttgc ggctgcggat cttgagcgtg
     2761 tagtcgtaga agtacgacgg gttgacccac gacacgtcgt tctgcccctt cgcgtcctgc
     2821 gacgcgaaga cctgattgcc cggcctgtcg tagagcgaga cgatgacgtt ctcgtccgcg
     2881 ccgtcgggga agaacttggg caggctctcg cggatgaggc gcggcatgag ctgcgccttg
     2941 aagtagtccg aatcgaggat catcccggtc gcgccgacga ccttccccca gtcgtccacg
     3001 accggcttga agaggacgcg gttctcgtgg tcgcggtcat cgacgtagag cttcgacgtc
     3061 ttgacgagcg tcccgtcctt cgccagcacg cgcagcggca ccgtggcgat ctgcgcggcg
     3121 cgcgcctcgc ccggcggcac gtcgccgagg cgcgtctggt cgtaggggct gtagaagacg
     3181 gtctgcggct cgccgcccgg cccgaacgag gagatgaaca gccgcttcgc gccctcgacc
     3241 ttgcacttcg agaagtggta cttcgtcttc aacaggtcgc cctgaatcga ggcggcggag
     3301 acgttcaacg actgctcggc gttcgtcgtg tagaacatct tgacctcctt cgacacgtcg
     3361 gcgaggtagt tcttcatccc gacgtgcccc gccacgatgg aggtcttctc cagcttcagc
     3421 agcgaccagt attggagcgc gatgatgctc agcagcggga cgaccaccgc cgccagcccc
     3481 acccagagga tgtgtctctt gaaaaatgtg cgcaacttcg tcatcacggt ctcacaccga
     3541 accccgcggc gcataacata caacggaacg gcgcccgcag tctctacccg gcgcgggctt
     3601 tttacaggaa gttgacaagt tttaccacta tttaccggcc tttgatgaca tttacgacgc
     3661 cgcccgctaa attcggcggc accaacaagc ggggaacgag gcggacggat ttcgaaggag
     3721 ccacgggcca tgaagcatct gcgattgtta cgactgacct gcgccgcata cctgctgttg
     3781 tgcgccgctt ctttcgccta ccgcgcggcg gcggcgaccg ttcacgacat ctccgccggg
     3841 agcggccacg cgcaaaccgc cacgccgcgg ccctgcctgc tgctccacgg caaacggctc
     3901 ttcaccctcg ccggctacga agtcaacgcc tccttcacgc ccggcggcaa gttccacagc
     3961 ttcgaattag tgagcagtaa gcgatgagca gtaggcagta ggcagcggaa gcaagaggct
     4021 tgagtcgtta cgtctcaagc ctcttgcttt actgcttgat gagcgaggag tccaagcacg
     4081 ctacctctta agctccttcg ctctgttgct cttgctgcct actgcctacc gccttctgcc
     4141 tactgctttc ttccggtgcc agccagaggg cgaggttgcg gatgtaggtc ttgatgtctt
     4201 cgagggcgcg cgggtctttc tcgtagccgt cgccgggcgg ctcggcgacg aagctcgggc
     4261 agcccttgtg cgggtcgaag ttgtaatcga cgaagtggtg gaagctcgac tcggcgacgg
     4321 cgcggcccgc ggcggtgtcg ccgtcgccgt tcgccccgcg ctcgaaggcg acgacgaggt
     4381 tgaaggggcg gcccgtgact ttgctcttgc cggtggcgac gacgcgagcg ccctccgcat
     4441 cgcgggggac gccgaccgcg ccctcgtgcg ggtgcgcggg gaagaggcgg atgaggccgt
     4501 ccgccgccgc cgggttgcgc aggagttcgt gcggcggctc ggtgagcgtg atctcctgat
     4561 agtcgccgtt gctgcccgag tggtagttcg gccacgagat gtcagtcgtc gccgtgtcgt
     4621 caacgcagcg gcgcgactcg tccggctcgg ggttcttcgt gtggaagaag tgcgcggcgc
     4681 cgacgccgcc gatggtgcag agacacgagc cgaggtcttc gtggtcgcgc gtcgagagga
     4741 tgccgccgcc gcgcccgcgg aagcgcgtga tccctgcgca gtcgccctcc gacagcccgt
     4801 cgcccgcgtc caccgcgaac agccacagct cgtcgtactc cgactcgtcg agcctgctga
     4861 gcacggggtc gttcccttcc gcgtcgcgct cgcggtcgcg ccccgtcacc tcgcacagcg
     4921 ccttgcccgc cccgtccttc aacgaagcga gctgctcgcg cagcatcgag aagcgcgcga
     4981 tgctccaatc gtccacggtc tccgggatgg tcgtctgcaa cagaatacga atcggactct
     5041 tcatgttcgt cagtctctac ttgggctgtt agctgtcagc taaacggttg acggatgacg
     5101 ggggcggcgc ttgtatcaat cgtctaccat cgatcctcaa gctaacagcc aacagctaac
     5161 agctaaacga ggacaatctt cccgtacgcg ccgggttcga ggacgacctc gtgcgcgcgc
     5221 gcggcgtcgg cgagcggcag ctcgcgcccg accacagggt tgagcgtcgc gttctcaagc
     5281 cccgcgccga tggcggcgtg gacgccctca agctcgcgcg gcgtggcgtt gaagagcgtg
     5341 aggccgtgga tgctcgcgtc gcgcgccatc aacgcgcgcg ggttgatctc gaccgtgccg
     5401 cgcgagccga cgacgaccac gcgcccgccc ttcgccagca ggtcgaggtc tttgccgagg
     5461 ttcacgttcg acagcatttc gaggatgatg tccacgccct cgccgccggt caacgtcttg
     5521 agctgttcga gatagtccgg cgcgcggtgg tcgagcacgt gatgcgcgcc ctgccccgcg
     5581 acgagtcgcc tgccctcctc ggtccctccc gtcccgatga ccttgaagcc cgcggcgcgc
     5641 gcgaactgca ccgccgccgt gccgacgccc ccgctcgcgc cgtgcaccag cacggactcg
     5701 cccgcccggc cctgcccgcg attgaacaag gcgcggtagg ccgtcgcata aggcgtgaac
     5761 acccccgcgc cctgcgcgaa cgagacgcgc tcgggaagcc tgtagacctg cgcctcctgg
     5821 cataccgcaa gctccgcgta cgcgccgctg acggtgcccg ccgtgtaaac cctgtcaccc
     5881 gccgcgacgc gcgtcacgcc cgcgcccacg gcctcgacca cgcccgccgc gtcgaggccc
     5941 ggcgtgtacg gcagcgacgg cttgatcgcg tgcccccccg aacggatgta cgtgtccacc
     6001 gggttcacgc ccgctgcgcg cacgcgcacc gacacctcgc cctcgctcgg ctgcgggtct
     6061 gccacctctt cgagccgcaa cacctccggc ccgccgaact cgtgcacgcg aatcgccttc
     6121 atcgcaatcc cccgctcaga aacttcgaga agtcagaagt cagaagtcag aagccagaat
     6181 gataaatgaa aagcaggttg ctttctattc atcgtgctga ctcccggctt ccgactcccg
     6241 acttccgact tctgacttct ccttaatact gccgggtgcg gcgggcgcga gattttatga
     6301 aacgtcgtat gacatccccg cgcccgccgc ccacgttgcg gcagttatga aacctgctgc
     6361 gccgcctcgg cgcgccagtc ctcctgcgcg cggaagcggc gaccgttgac gttggccgac
     6421 tcgtccgagg cgaggaagac gaagacctcc gtcacctccg cggggtcggc ccactgcgtc
     6481 gggtcttctt cgggttcggc gtcgcggtgc atcttcgtgt tcatctcgcc ggggtcaacc
     6541 cagttgacgc gcacgccgct gccttcgagt tccgccgccc acgtctcgct caggccctcg
     6601 atgccgaact tcgagatgcc gtaggcgccc cagccggggt agcccgtgcg gcccgcgtcg
     6661 ctcgtcacgt tgatgatcga gccgcccgcc tcgaccatcg ccggcagcgc cttcttgatg
     6721 agcaggaacg gcgccaccag attcgtttcg agcaccgccc ggaagtcttc gagcgggtag
     6781 tcgagcagga aaggcagcgg caccgggccg atgatcgaag cgttgttcac gagcacgtcc
     6841 agccgcccgc cgaactccgc cagcgcgtcg gcgacgaggc gctccacgtc ctcgtgtctg
     6901 cgcaggtccg cggcgaccgc gagcacgcgc gcccccggcg ccgcctcctc gatggcggcg
     6961 cgcacctctt cgagcgcatc cttcgtccgc gccgagatga ccagcgccgc cgcgccctcg
     7021 cgcgcgaact caagcgcgag ctgccgaccc aatccctgcg acgcgcccgt aatcagtacg
     7081 tgttttcctt ccagtccgtt catcgcttcg acgcctccca cgcggtcggg c
//
LOCUS       DBNGOH010000490         3677 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_621667, whole genome shotgun sequence.
ACCESSION   DBNGOH010000490 DBNGOH010000000
VERSION     DBNGOH010000490.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3677)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3677)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3677
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_621667"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..986)
                     /locus_tag="AC159P_09705"
     CDS             complement(<1..986)
                     /locus_tag="AC159P_09705"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HIR3695767.1"
                     /translation="MSSQPTVMKFGGTSVEDARAFERVRRVVAAREAERPVVVVSAMG
                     GFTDALLEAVGAASEGDAEAAAQALEPQFERHVEVARALLTEGAAGEVAATVEAARRG
                     VAEALGAVAAQSKPPALLQDEVVSYGERLSAKLLAHVLRGAGLRARCVDARRCIRTDA
                     EHGRATPHKDETERRTCEELAHLVEAGEVPVMGGFIASSAEGLTTTLGRGGSDYPAAL
                     VGAALRAREIQIWTDVSGVLTADPRIVREARTIPRLSYAEAAELAYFGAKVLHPKTIL
                     PAVVRNIPVRICNSRDAQAPDTVVYFDAEMSPRTVKAIARKKGVHIVHVTSAR"
     gene            complement(1301..>3677)
                     /locus_tag="AC159P_09710"
     CDS             complement(1301..>3677)
                     /locus_tag="AC159P_09710"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 94 protein"
                     /protein_id="HIR3695768.1"
                     /translation="PWTNVVANDRDFGFIVTEGGGGYTWSANSRENRLTPWSNDAVSD
                     TPGECLYLRDEESGTVWSPTPLPIRETEPYVVRHGQGYTVFEHTSHGLSQELLMFVPV
                     DAPVKISVLRLRNRSERPRRVSVTSYSELVLGVTRGASAPYVITEYNRTRAALFARNP
                     YNNEFAARVAFAATSAPRFTLTCDRKEFLGRNGTLERPAALRRTELSGRVGAGLDPCV
                     AIQTVFDLAPGETREVAFLLGEGESEEEARAVVTRFRQLQTVYEAFEKMLARWDEILE
                     AIEVKTPDAARDTILNRWLLCQTLACRVWARSAFYQSGGAYGFRDQLQDVMALVYSEP
                     AIARAQIVRAAARQFREGDVQHWWHPPTGRGVRTRFSDDLLWLPYVTSFYVEKTGDTS
                     VLDETAPFIEAPLLAEGEDETYTQPVVAEERATVYEHCARTLDRSLAVGEHGLPLMGS
                     GDWNDGMNRVGHEGRGESVWVGWFLHNTLEWFTPFCEQRGDAERAEGYRAHMEKLKVA
                     LRDNAWDGDWYRRAYFDDGTPLGSVQNDECRIDSIAQSWGVISGASEQHRAHRAMAAV
                     EEYLIRRGDGIIMLFTPPFDRSALDPGYIKGYVPGVRENGGQYTHAAIWTVIAYALLG
                     DGDKAGELFALLNPINHASTRAGLHKYKVEPYVAAADIYAVWPHTGRGGWTWYTGSAG
                     WMYRAGLEFILGFELRGDALRINPCVPRSWRDYEISYRHRTARYHIKVENPYGVNRGV
                     VSVELDGTPLPSHDIPLADDGQRHNVRVLMGEPAPQASDQEPRAEAAQKRA"
BASE COUNT          540 a         1243 c         1315 g          579 t
ORIGIN      
        1 cgcgccgagg tgacgtggac gatgtggacg cccttcttgc gcgcgatggc cttgacggtg
       61 cgcggcgaca tctcggcgtc gaagtagacg acggtgtcgg gcgcctgcgc atcgcgcgag
      121 ttgcagatgc ggacggggat gttgcggacg accgcgggca ggatcgtctt cgggtgcaag
      181 accttcgcgc cgaagtaggc cagctccgcc gcctccgcgt aggagaggcg cgggatggtg
      241 cgcgcctctc tgacgatgcg cgggtcggcg gtcaaaacgc ccgacacgtc cgtccagatt
      301 tgaatctcgc gcgcgcgcaa cgccgcgccg acgagcgccg ccgggtagtc cgagccgccg
      361 cgcccgagcg tcgtcgtcag gccttccgcc gaagaggcga tgaagccgcc catgacgggc
      421 acctcgcccg cctcgacgag gtgcgccagc tcttcgcacg tgcgccgctc ggtctcgtcc
      481 ttgtgcgggg tggcgcgccc gtgctcggcg tccgtgcgga tgcagcgccg cgcgtccacg
      541 caccgcgcgc gcagacccgc accccgcagc acgtgcgcca gcagcttcgc cgacagccgc
      601 tcgccgtaag agacgacctc gtcttgcagc agcgccggcg gcttcgattg cgcggccacc
      661 gcgccgagcg cttccgccac tccgcggcgc gcggcctcga ccgtcgccgc gacttcgccc
      721 gccgccccct cggtcaagag ggcgcgcgcg acttcgacgt gtcgctcgaa ctgcggttcg
      781 agcgcctgcg ccgccgcttc ggcgtccccc tcggaggccg cgcccacggc ttcgagcagc
      841 gcgtccgtga acccgcccat cgccgagacg accacgacgg gcctctcggc ctcgcgcgcg
      901 gcaacgacgc gccgcacgcg ctcgaacgcg cgcgcatcct cgacggacgt gccgccgaac
      961 ttcatcaccg tcggttgtga agacatcgcc tgacccgaaa gacgcgcgcc gggccgagca
     1021 gccccgcgcc ccactcatca cttccgctta tgaagattat aagtcccgcc gtaaggatac
     1081 acgacagacc tcaagaaccg aaagcacgga cgattcgacg aaggaggaaa aggtcaagag
     1141 gggcagaggg gaagagggag gatgagaagc atgctgttac tcgtcctcaa ccggacatga
     1201 aaagcagcgt gcttctcatc gagttgatga tgagaggcac gctgcttctg tcttcccttt
     1261 tcgcctcttc ccctttcgac cttttaccct ttgtcgagca ttacgcccgc ttctgtgcgg
     1321 cctcggcgcg cggctcctgg tcggatgcct ggggggcggg ttcgcccatg aggacgcgga
     1381 cgttgtggcg ctggccgtcg tcggcgaggg ggatgtcgtg ggaagggagc ggcgtgccgt
     1441 cgagttcgac ggagacgacg ccgcggttga cgccgtaggg gttctcgacc ttgatgtggt
     1501 agcgggcggt gcggtggcgg taactgatct cgtagtcgcg ccacgagcgc gggacgcagg
     1561 ggttgatgcg gagcgcgtcg ccgcgcagct cgaagccgag gatgaactcc agaccggcgc
     1621 ggtacatcca gccggcggag ccggtgtacc acgtccagcc gccgcggccc gtgtgcggcc
     1681 agacggcgta gatgtcggcg gcggcgacgt acggctcgac cttgtacttg tgcagcccgg
     1741 cgcgcgtcga ggcgtggttg atcgggttca gcagcgcgaa cagctcgccc gccttgtcgc
     1801 cgtcgccgag caaggcgtag gcgatgaccg tccagatggc ggcgtgcgtg tactggccgc
     1861 cgttctcgcg cacgccgggc acgtacccct tgatgtaacc ggggtcgagc gcgctgcggt
     1921 cgaagggcgg cgtgaagagc atgatgatgc cgtcgccgcg gcggatcaga tactcttcga
     1981 cggcggccat cgcgcggtgc gcgcggtgct gttccgaagc gccggagatg acgccccagc
     2041 tctgcgcgat ggagtcgatg cggcactcgt cgttctggac ggagccgagc ggcgtgccgt
     2101 cgtcgaagta agcgcggcga taccagtcgc cgtcccacgc gttgtcgcgg agcgcgacct
     2161 tgagcttctc catgtgcgcg cggtagcctt cggcgcgctc ggcgtcgccg cgctgttcgc
     2221 agaagggcgt gaaccattcg agcgtgttgt ggaggaacca gccgacccag acgctctcgc
     2281 cgcgcccctc gtggccgacg cggttcatgc cgtcgttcca gtcgccggaa cccatgagcg
     2341 gcaggccgtg ctcgccgacg gcgaggctgc ggtcgagcgt gcgcgcgcag tgctcgtaga
     2401 cggtcgcccg ttcctccgcg acgacgggct gcgtgtaggt ctcgtcctcg ccctcggcca
     2461 agagcggcgc ttcgatgaag ggcgcggtct cgtcgagcac cgacgtgtcg cccgtcttct
     2521 cgacgtagaa gctcgtcacg taaggcagcc acagcaggtc gtccgagaag cgcgtgcgga
     2581 cgccgcgccc ggtgggcggg tgccaccagt gctgcacgtc gccctcgcgg aactggcgcg
     2641 cggcggcgcg cacgatctgc gcgcgcgcga tggcaggctc ggagtagacg agcgccatca
     2701 cgtcctgcaa ctggtcgcgg aagccgtagg ccccgcccga ttgataaaag gccgagcgcg
     2761 cccagacgcg gcaggcgagc gtctggcaga gcagccagcg gttcaagatc gtgtcgcgcg
     2821 ccgcgtcggg cgtcttcacc tcgatcgcct ccaggatttc gtcccagcgc gcgagcatct
     2881 tctcgaacgc ctcgtagacg gtctgcaact gcctgaagcg cgtgacgacg gcgcgcgcct
     2941 cttcctccga ttcgccctcg ccgagtagaa acgcgacctc gcgcgtctcg ccgggcgcga
     3001 gatcgaacac ggtctgaatc gcgacgcacg ggtccagccc cgcgccgacg cggcccgaaa
     3061 gctccgtgcg gcggagcgcc gccgggcgtt cgagcgtgcc gttgcggccc aggaactcct
     3121 tgcggtcgca ggtcagcgtg aagcggggcg cgctcgtcgc ggcgaaggcg acgcgcgcgg
     3181 cgaactcgtt gttgtagggg ttgcgcgcga acagggcggc gcgcgtgcgg ttgtattcgg
     3241 tgatgacgta aggggcgctg gccccgcgcg tgacgccgag gacgagttcg ctgtagctcg
     3301 tcacggagac gcggcgcggg cgctcgctgc ggttcctgag acgcagcacg gagattttga
     3361 cgggcgcgtc cacggggacg aacatcagca gctcttgcga gaggccgtgg ctcgtgtgct
     3421 cgaagacggt gtagccctgc ccgtggcgga cgacgtacgg ctcggtctcg cggatgggca
     3481 gcggcgtcgg cgaccagacg gtgccgctct cctcgtcgcg caggtaaagg cactcgccgg
     3541 gcgtgtcgct gacggcgtcg ttcgaccacg gcgtgagacg gttctcgcgg ctgttcgccg
     3601 accacgtgta gccgccgccg ccttcggtga cgatgaagcc gaagtcgcgg tcgttggcca
     3661 ccacgttcgt ccacggc
//
LOCUS       DBNGOH010000491         2192 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_255213, whole genome shotgun sequence.
ACCESSION   DBNGOH010000491 DBNGOH010000000
VERSION     DBNGOH010000491.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2192)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2192)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2192
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_255213"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..304
                     /locus_tag="AC159P_09715"
     CDS             <1..304
                     /locus_tag="AC159P_09715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695769.1"
                     /translation="SRAPEMSAPRAVPRSEPNVSPYRGGERSYPSPERSRPGGGAEHV
                     VQVSMKVEQGRVVEAAVKNPRAGMASYEALALRLARQRRYPDAFNGPDTLQLKVKP"
     gene            327..545
                     /locus_tag="AC159P_09720"
     CDS             327..545
                     /locus_tag="AC159P_09720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014397989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695770.1"
                     /translation="MNHANLGPREVARIERELAGQQNLNDVMRWALSHPKGVFIPSVV
                     AQVIVQDEFTHDVIVPYREGLVLVYDTT"
     gene            570..710
                     /locus_tag="AC159P_09725"
     CDS             570..710
                     /locus_tag="AC159P_09725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695771.1"
                     /translation="MWDHLPSADEILAGRLAAGWQPTPSALKDGDRVLGHAACAVVDK
                     PT"
     gene            832..2166
                     /locus_tag="AC159P_09730"
     CDS             832..2166
                     /locus_tag="AC159P_09730"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014081.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR3695772.1"
                     /translation="MPETDGGYCVGETTRRSFLAGAAGALVGLALGPEAGGRQPSSKA
                     YEFINGRWFDGRQFRDGRFYSDGGVLTTKRLARAERVFDLAGKYVVPPFGEAHNHNLD
                     WSSDEQFARVKRMYLEGGVFYVKNPTNLPRAAAPLVGKVNIPTSVDVIFAHGGLTASG
                     GHPLGLVRRNIERGGMTEADADGGFCWIIDTVADLDRKWPRIRAGKPDFIKTTLVYSE
                     EFEKRRRDEKYFSWRGLDPALLPAIVRRAHSDGLRVSTHVESATDFHHALVAGADEIN
                     HTPGFRPEGNDFANYRELKRYEITESDARLAARRRVVVVTTMGTSINLSFDEKQQTPE
                     LRAAVREMLARNLRLLHRHGVPIAVGSDSFRQTSVPEALSLHRLGVFDNLTLLKMWCE
                     TTAAAIFPKRKIGHLKPGSEASFLVLGGDPLQDFTNVQKIETRIKQGELLSV"
BASE COUNT          392 a          735 c          740 g          325 t
ORIGIN      
        1 gtcgcgcgcg ccggaaatgt ctgcgcctcg cgccgtcccg cggtccgagc cgaacgtctc
       61 gccctatcgt ggcggcgagc gcagctaccc gtcgccggaa cgctcgcggc ccggcggcgg
      121 cgcggagcat gtcgtgcagg tctccatgaa ggtggagcag ggaagagtcg tcgaggcggc
      181 ggtcaaaaac cctcgggccg ggatggcgag ctacgaagcg ctggcgctca ggctggcccg
      241 gcagcgccgc tacccggacg cctttaacgg gcctgacaca ctccaactca aggtaaagcc
      301 gtaggccgag ccggaatatg cgagtcgtga atcacgccaa ccttgggcct cgggaagtcg
      361 cgcggatcga gcgcgagctg gcggggcagc agaatctcaa cgacgtgatg aggtgggcgc
      421 tgtcgcatcc gaagggcgtc ttcatcccct cggtcgtcgc acaggtaatc gtgcaggacg
      481 agttcacgca tgacgtgatc gtcccgtacc gcgaggggct ggtgctcgtc tacgacacga
      541 cctgactggg cggcgtcacg gcagttgccg tgtgggatca tctgccgagc gccgacgaga
      601 ttctggccgg gcgtctcgcc gccgggtggc agccgacgcc gtcagcgctg aaggacggcg
      661 acagggttct cggccacgcg gcctgcgcgg tggtggacaa accgacgtag cacatcccgg
      721 ctcacactgc ccttcgccgg ccccgccgcg gcgacacaaa atttctgcca ctaaattcag
      781 cccgcgcggt gtctctcccg tagaagactt gaacaaaaga acggaggggc catgcctgaa
      841 acggacggcg gctattgtgt cggagagact acgcggaggt ctttcctcgc gggcgccgcg
      901 ggcgccctcg tcggcctcgc actggggccg gaggcgggcg ggcggcagcc ttcgtcgaag
      961 gcatacgaat tcatcaacgg gcggtggttc gacgggcggc agttcaggga cggaaggttc
     1021 tattcggacg gcggcgtctt gaccaccaag aggctcgcgc gcgcggagcg cgtcttcgac
     1081 ctcgcgggca agtacgtggt gccgcccttc ggcgaagccc acaaccacaa cctcgactgg
     1141 tcgagcgacg agcagttcgc gcgcgtcaag cgcatgtacc tcgaaggcgg cgtcttctac
     1201 gtcaagaacc cgaccaacct tccgcgcgcg gcggcgccgc tcgtcggcaa ggtcaacatc
     1261 ccgaccagcg tcgatgtcat cttcgcgcac ggcggcctga ccgcctcggg cgggcacccg
     1321 ctcggcctcg tgcggcgcaa catcgagcgc ggcgggatga ccgaggccga cgccgacggc
     1381 ggcttttgct ggatcatcga cacggtggcc gacctcgacc gcaagtggcc tcgcatcagg
     1441 gcgggcaagc ccgacttcat caagacgacg ctcgtctatt cggaggagtt cgagaaacgc
     1501 cggcgcgacg aaaaatactt tagctggcgc ggactcgacc ccgcgctgct ccccgccatc
     1561 gtccggcgcg cgcacagcga cggcctgcgg gtctcgaccc acgtcgagtc ggcgaccgac
     1621 ttccatcacg ccctcgtcgc gggcgccgac gagatcaacc acacgcccgg cttcaggccc
     1681 gaaggcaacg acttcgcgaa ctacagggag ctgaagcgct acgagatcac cgaatcggac
     1741 gcgcggctcg cggcgcgccg ccgcgtcgtg gtcgtcacca cgatggggac gagcatcaat
     1801 ttatcgttcg acgaaaaaca gcagaccccg gagttgcgcg ccgccgtgcg cgagatgctc
     1861 gcacgcaacc tgcggctgct gcaccgacac ggcgtcccca ttgccgtcgg gagcgacagc
     1921 ttccgtcaga cctccgtgcc cgaagcgctg agcctgcaca ggctcggggt cttcgacaac
     1981 ctgacgctgc tgaagatgtg gtgcgagacg acggcggcgg ccatcttccc caagcgcaag
     2041 atcggccacc tgaagcccgg ctccgaggcg agcttcctcg tgctcggcgg cgacccgctt
     2101 caggatttca cgaacgtcca gaagatcgag acgcggatca aacagggcga gctgctgtct
     2161 gtgtgacggc gaccgaaggg cgcgagtccg gg
//
LOCUS       DBNGOH010000492         4153 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_72448, whole genome shotgun sequence.
ACCESSION   DBNGOH010000492 DBNGOH010000000
VERSION     DBNGOH010000492.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4153)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4153)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4153
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_72448"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..734)
                     /locus_tag="AC159P_09735"
     CDS             complement(<1..734)
                     /locus_tag="AC159P_09735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695773.1"
                     /translation="MREAMPVLMRAVYSLAFVVLVAGAEAAVAQGVLTGDWTASTRTE
                     ESDKINLSFERRTERGGRSQMGSTYAYADLQGLSREQALGGGAVRFSIVREAGTIDCE
                     GSFQNGRGSGTFRFTGNQSFVSAMKSRGFDFETKSSASHHERDSENRLFTATTLNVTT
                     ALADDLLSADFGKLDVDDLFKAAIFKVDSKFMRELKATGFPNLRMEELVKARIFKIDA
                     DFVKQVADMGLGKEESFESLVKMRIF"
     gene            1305..4004
                     /locus_tag="AC159P_09740"
     CDS             1305..4004
                     /locus_tag="AC159P_09740"
                     /inference="COORDINATES: protein motif:HMM:NF012801.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR3695774.1"
                     /translation="MRQTQRGSVEGVVRDDGGGAVVGARVQLLGPQQTVVKATETDAE
                     GRFRFADVPAGSYVLRVEHGGFAARRVAADVSHGGGATVNVTLSVEALSEQVTVTAEA
                     GQVADARSIDQQVNVISQKEILDRAPEVVAQVVDEEPGVNLQRTSPSLSAVFVRGLTG
                     RNVAVYVDGVRYTTSAQRGGVGTFFSLVEPSSLETVEILRGPNSSQYGSDVLGGVVNF
                     IPHAPSYGALDDNFHGHTNLFYTSPTNGFGGSTLLTYGARRYGVLLNLNARRINRLRP
                     GGGIDGHSALTRFLGIPSDVLGERLPDTAYTQYGGLLRASVSLDDTTQLLFSYSRNQQ
                     DGGRRYDQLLGGDGNLIADLRNLMTDLFYVRLAKQDLGFFDNATFTFSYNGQREERVN
                     QGGQGDPTGTITSQRERTNVYGFNFHLDKQLGERNTALFGADVYRDGISAPAYTLTPS
                     ARVTTRSRPRVPDGARYLTYGVFVQDVFTAVPERLRLSGALRYNVASYRSRASNAPLD
                     AAGRPLFPDDSARFAAFSGRFGAVVSVGGGFDIAAKYTRGFRAPNTTDLGIIGLVGTG
                     FEVDASTAAGLGGFIGTTAGADAVSSGIPITRLGPETSDSFEISFRLTRSRVRAELTG
                     FTNKLKDVYFDQALILPQGAVGTFLGSERITAQNPVTGTVFVAGVTSPVLVRVNYDDA
                     RFNGVEFHSEARLSRDFTTNANFTYVRAHSLLNGLPPNVEGGVPPATAFVSLRYQPRA
                     TTRFYVEAYSTLAARQRRLSTLDLADRRTGAARTRGQIQNFFRRGACVRGITMPGAAG
                     CGSAGGILVATGETLAEVQNRLLPIGATINGVTVVDNASAVPLFNAVPGYGLVGLRGS
                     VRIGERSEVLLDFENIADKSYRGISWGVDGAGRGLTLRYRLKF"
BASE COUNT          676 a         1534 c         1257 g          686 t
ORIGIN      
        1 ttgaagatgc gcatcttgac gaggctctcg aacgactcct ccttccccag acccatgtcc
       61 gctacctgct tgacgaagtc ggcgtcgatt ttgaagatgc gcgccttcac cagctcctcc
      121 atgcggaggt tcgggaagcc ggtcgccttc agctcgcgca tgaacttcga atcgactttg
      181 aagatcgccg ccttgaaaag gtcgtccacg tcgagcttgc cgaaatccgc cgagagcaga
      241 tcgtcggcca acgccgtcgt gacgttcagc gtcgtcgccg tgaatagtct gttctcggag
      301 tcgcgctcgt ggtgcgacgc cgaagacttc gtttcgaagt cgaagccccg gctcttcatc
      361 gccgagacga aactctggtt gcccgtgaaa cggaaggtgc cggagcctct gccgttctga
      421 aagcttccct cgcagtcgat ggtcccggcc tcgcgcacga tgctgaatct gacggccccg
      481 ccgcccaggg cttgctcgcg gctcaggccc tgcaagtcgg cgtaagcgta ggtcgaaccc
      541 atctgactcc tgccgcctct ctcggtgcgc cgctcgaacg agaggttgat tttgtccgac
      601 tcttcggtcc tggtcgaagc cgtccagtcg cccgtcagca cgccctgcgc aacggcggcc
      661 tcggcaccgg cgaccaacac gacgaaggcc agactgtaaa cagcacgcat caaaaccggc
      721 atcgcttcac gcatggcatc atctcctcga agtcgaaatt tttccgtctt gcaacaagga
      781 aacaccgcgg ggcagggatt cgtggcaggg aatttcgccc tgctctcgct ccaggccctc
      841 gaacgagttc gctgcccggc gtctgccgcc gagcttcgaa gcgaattcgc ttacataaaa
      901 gcgaactcag gccaacattt cggattccgc ggcggaagcc ggtagggcgt gctcatcctt
      961 tgccggaagc tagccatgct ataaactaac gtaccgaggc cgacgcagcc gaaccgaggt
     1021 ggccgaagac cgtggctccg cgactttact ctttgtctct cctccgccga ccaccttcgt
     1081 cgtcaactcc actcgccagc gggttcgcgt gtccggcgcg gggcgccgcg gcgaaccaaa
     1141 gcgcgcggtc ggtgttgctc cggtaagcta tcgtcgctcc gcttcccgta cacttcgcac
     1201 gctcgacact ccttccagac gagagggttg gtatgaatgt tccgaggttg tgtttgctgg
     1261 cactgctcct gctcgcaccg tgcgcgacgg gggcggatgc acgcgtgcga cagacacagc
     1321 gcgggagcgt cgaaggcgtc gtgcgggacg acggcggcgg cgcggtcgtg ggcgcgcgcg
     1381 tccaactgct cgggccgcag cagaccgtag tcaaggcgac cgaaacggac gccgaaggcc
     1441 gcttccgctt cgcggacgtg cccgccggct cgtacgtcct gcgcgtcgag cacggcgggt
     1501 tcgccgcgcg gcgcgtggcg gcggacgttt cgcacggcgg cggggcgaca gtcaacgtca
     1561 cgctcagcgt cgaggcgctc tccgagcagg tcacggtgac ggcggaggcc gggcaggtcg
     1621 ccgacgcgcg gagcatcgac cagcaggtca acgtcatcag ccagaaggag attctcgacc
     1681 gcgcgcccga agtcgtcgcg caggtcgtgg acgaagagcc gggcgtcaac ctgcaacgca
     1741 cgagtccgtc tctgagcgcc gtcttcgtgc gcgggctgac gggccgcaac gtcgccgtct
     1801 acgtggacgg cgtgcgctac acgacgagcg cgcagcgcgg cggcgtcggc accttcttca
     1861 gcctcgtcga gccttcgagc cttgagaccg tcgaaatcct gcgcggcccg aacagctcgc
     1921 agtacggctc ggacgtgctc ggcggcgtcg tcaacttcat cccgcacgcg ccctcctatg
     1981 gcgccctcga cgataacttc cacggccaca ccaacctctt ctacacgtcg ccgaccaacg
     2041 gcttcggcgg gagcacgctg ctgacctacg gcgcgcggcg ttacggcgtg ctcttgaacc
     2101 tcaacgcgcg gcgcatcaac cgcctgcgcc ccggcggcgg catcgacggc cactcggccc
     2161 tgacgcgctt cctcggcatc ccctcggacg tgctcggcga gcgcctgccc gacaccgcct
     2221 acacgcagta cggcgggctg ttgcgcgcca gcgtcagcct cgacgacacg acgcaactgc
     2281 tcttcagcta ttcgcgcaac cagcaggacg gcgggcgccg ctacgaccaa ctgctcggcg
     2341 gcgacggcaa cctcatcgcc gacctgcgca acctgatgac cgacctcttc tacgtccgcc
     2401 tcgccaaaca agacctcggc ttcttcgaca acgccacgtt caccttctcc tacaacggcc
     2461 agcgcgaaga gcgcgtcaat cagggcgggc agggcgaccc cacgggcacc atcacctcgc
     2521 agcgcgagcg cacgaacgtc tacggtttca acttccacct cgacaagcag ctcggcgagc
     2581 gcaacaccgc gctcttcggc gcggacgttt accgcgacgg catctccgcc ccggcttaca
     2641 cactgacccc ctcggcgcgc gtgacgacgc gctcgcgccc gcgcgtgccg gacggcgcgc
     2701 gctacctcac ttacggcgtc ttcgtgcagg acgtgttcac cgccgtcccc gaacgactgc
     2761 gcctgagcgg cgcgctgcga tacaacgtcg cttcgtacag gtcgcgcgcc tcgaacgcgc
     2821 cgctcgacgc ggcgggccgc ccgctcttcc ccgacgacag cgcgcgcttc gccgccttct
     2881 cgggacgctt cggcgccgtc gtcagcgtcg gcggcggctt cgacatcgcc gcgaagtaca
     2941 cgcgcggctt ccgcgccccg aacacgaccg acctcggcat catcggactc gtcggcaccg
     3001 gcttcgaagt tgacgcctcg accgccgccg gcctcggcgg cttcatcggc acgaccgccg
     3061 gcgccgacgc cgtctcgtcc ggcatcccca tcacgcgcct cggccccgag acgagcgaca
     3121 gcttcgagat cagcttccgc ctcacgcgct cgcgcgtccg ggccgagttg accggcttca
     3181 cgaacaagct caaggacgtt tacttcgatc aggcgctcat cctcccgcag ggcgcggtgg
     3241 gcacgttcct gggcagcgag cggatcaccg cccagaaccc tgtgaccggc accgtcttcg
     3301 tcgcgggcgt gaccagcccc gtgctcgtgc gcgtgaacta cgacgacgcg cgcttcaacg
     3361 gcgtcgagtt ccactccgaa gctcgtctct cgcgcgactt cacgacgaac gccaacttca
     3421 cctatgtccg cgcgcacagc ctcttgaacg gcctgccgcc gaacgtcgaa ggcggggtgc
     3481 cgcccgcgac cgcctttgtc agcctgcgct accagccgcg tgcgaccacg cgcttctacg
     3541 tcgaagccta ctcgacgctc gccgcgcgcc agcgccgcct ctccacgctc gacctcgcag
     3601 accgccgcac cggcgcggcg cgcacccgcg ggcagattca aaacttcttc cgccgcggcg
     3661 cctgcgtccg cggcatcacc atgccgggcg ccgccggctg cggctccgcg ggcggcatcc
     3721 tcgtcgccac cggcgagacg ctggccgagg tccagaaccg cctgctgccc atcggcgcga
     3781 ccatcaacgg cgtcacggtc gttgacaacg ccagcgccgt cccgctcttc aacgccgtcc
     3841 ccggctacgg cctcgtcggc ctgcgcggct ctgtgcgcat cggcgaacgc tccgaggtgc
     3901 tgctcgactt cgagaacatc gccgacaaat cgtaccgcgg catcagttgg ggcgttgacg
     3961 gcgcgggccg cggcctcacg cttcgttacc gcctcaagtt ctgacacgcc cgtgatggag
     4021 agcgccggac ggaaggggaa gagggcaaga ggggaaaagg cgaagaggtt ttttgtcctc
     4081 accctcctcg ccttttcccc tcttaccctg ttcccctctc cccgcccggc gccctctcaa
     4141 ccatgcacaa aga
//
LOCUS       DBNGOH010000493         3823 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2822406, whole genome shotgun sequence.
ACCESSION   DBNGOH010000493 DBNGOH010000000
VERSION     DBNGOH010000493.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3823)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3823)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3823
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2822406"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1422
                     /gene="ppk1"
                     /locus_tag="AC159P_09745"
     CDS             <1..1422
                     /gene="ppk1"
                     /locus_tag="AC159P_09745"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF003921.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HIR3695775.1"
                     /translation="HADMLFPRMVAGQTYAFRVTRDADVEIREDEADDLLRSMEATLR
                     RRRFGVAVRLEISASMPPEMVEYLTESLKLAADDVYQIDGPLNVPDLMSLYKINRPEL
                     KDEPFRPKTPEVLRRGGSIFELVKKQDVLLHHPYVSYTVVTDFINEAARDPNVVAIKM
                     CLYRTGQNSPIPKALIEACEREKQVTALVELKARFDEEFNIEWAKKLEEAGVHVVYGI
                     VGLKTHSKVSLVVRQEPEGLRRYVHVATGNYNPVTSNFYTDLSLFTADEEIGADATEL
                     FNFLTGFSRQKEYRQLLVAPVNLRQKMLALIHREAEHARAGRPARIAVKINRLADIRI
                     INSLYEASQAGVRIDLIVRGICMLRPGVPGLSETITVRSVVGRFLEHSRVYYFANGGD
                     EEFYVGSADWMPRNFDRRVEVIAPVHDPRLKDHLKNVMLAAYLRDNVKARRLRTDGLY
                     ERFPAAEGEERFDAQTYFFRAEK"
     gene            1633..2598
                     /locus_tag="AC159P_09750"
     CDS             1633..2598
                     /locus_tag="AC159P_09750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695776.1"
                     /translation="MLSNRSVRRHLVLAVIVALSVSSPTLAIQKKEKDKPAPRGTPVL
                     WREPVDIASRDLFLGPGGASMRPDLRGVTFVKEEKGGYSTKYRVKDGAGRVWVAKIGK
                     EAQSETAAVRLLWAVGYPTEINYLVPRLTIEGKGTFENVRLEARPESVDRLDEWKWSD
                     NPFVGTKELQGLIVLMALLNNWDIKDANNKVFHTRGELRYVISDLGATFGRVKSGAPI
                     VWRIRRNRNDPADYSEDPFVEDVKRGNVLLFYKGKRQDLFDDIRVEEARWIGGWLARL
                     SDRQLADAFRAANYTPGEVKLLVGAVRARINELTSLPAGGLANRR"
     gene            2745..3689
                     /locus_tag="AC159P_09755"
     CDS             2745..3689
                     /locus_tag="AC159P_09755"
                     /inference="COORDINATES: protein motif:HMM:NF027640.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HIR3695777.1"
                     /translation="MRNNQKVLIVAALGAAVIGFGIVALVFRAREAERPARGVDTVGV
                     LASPTQPAATPPQQTTTAPTATPRAKDSNPLLDLLQGEEVTTTAEQQPRGVVETLLSV
                     TLRLTLAAVLAAMLAFRPRRVRRIIRRNPFVAQTQILLAVVSSALMMIVGDSAARAFG
                     IFAAVSFVRFRTNIRDPKEVTVLLVSLAIGLATGTGRWELAIILSFFILLLLWGLEYR
                     EPELLFRAMELTVKTKNVDMTQEVLLRLFRKYRFDAEMRTIDRPDAEDEVGCVVYHVN
                     VSPLISTDRLSEEIFSADSANIDSVEWDQQKNPAHYYQ"
BASE COUNT          713 a         1275 c         1223 g          612 t
ORIGIN      
        1 cacgccgaca tgctcttccc gcggatggtc gcgggccaga cgtacgcctt ccgcgtcacg
       61 cgcgacgccg acgtggagat acgcgaggac gaggccgacg acctgctgcg ctcgatggag
      121 gcgaccctca gacgccgccg gttcggcgtc gccgtgcggc tcgaaatctc cgcctcgatg
      181 ccgccggaga tggtcgaata cctgaccgaa tccctgaagc tcgccgcgga cgacgtgtac
      241 cagatcgacg gcccgctcaa cgtccccgat ctgatgtcgc tctacaagat caaccgcccc
      301 gagctgaagg acgagccgtt ccgcccgaag acgcccgagg tgctgcggcg cggcggctcg
      361 atcttcgagc tggtcaagaa gcaggacgtg ctgctgcacc acccgtacgt ctcctacacg
      421 gtcgtcaccg acttcatcaa cgaggccgcg cgcgacccca acgtcgtcgc catcaagatg
      481 tgcctctacc gcacggggca gaactcgccg atcccgaagg cgctgatcga ggcgtgcgag
      541 cgcgagaagc aggtgacggc gctcgtcgag ctgaaggcgc gcttcgacga ggagttcaac
      601 atcgagtggg cgaagaagct cgaagaggcg ggcgtccacg tcgtctacgg catcgtcggc
      661 ctgaagacgc acagcaaggt ctcgctcgtc gtccggcagg agccggaagg cttgcgccgc
      721 tacgtccacg tcgccacagg caactacaac ccggtcacgt cgaacttcta cacagacctc
      781 agcctcttca cggcggacga agagatcggc gccgacgcca ccgagctgtt caacttcctg
      841 accggcttct cgcggcagaa ggagtaccgg caactgctgg tcgcgcccgt caacctgcgc
      901 cagaagatgc tcgcgctcat ccaccgcgag gccgagcacg cgcgcgcggg gaggcccgca
      961 cgcatcgctg tcaagatcaa ccgcctcgcc gacatccgca tcatcaactc gctctacgag
     1021 gcgtcgcagg cgggcgtcag gattgatttg atcgtgcgcg gcatctgcat gttgagaccg
     1081 ggcgtgccgg gtctgtcgga gacgatcacg gtgcggagcg tcgtcgggcg cttcctcgaa
     1141 cacagccgcg tctactactt cgccaacggc ggcgacgagg agttctacgt cgggagcgcc
     1201 gactggatgc cgcgcaactt cgaccgtcgc gtcgaggtca tcgcgcccgt ccacgacccg
     1261 cgcctcaaag atcacctcaa gaacgtcatg ctggcggcct acctgcgtga caacgtgaag
     1321 gcgcggcggc tgcgcaccga cggcttgtac gagcgcttcc ccgccgccga aggcgaagag
     1381 cgcttcgacg cgcagaccta cttcttccgc gcggagaagt agaaaggccg tgaaccgtaa
     1441 accggtaaaa gccgtgaacc gtaaaccgtc aaccgtgata agtgaagagc cgtgattggt
     1501 aaactgtgaa ccgataagag ccgtgaggcg tgaattgcga accgcgacga gtgagaacgt
     1561 ttttcttact tggcacggtt cacggtttac ggttcacggc tcttgaattt cttcaagggg
     1621 aggccaaggg tcatgttgtc gaatcggtct gtgcgccggc atctcgtgct ggcggtcatc
     1681 gtcgctctct ccgtctcctc tcccacgtta gcgattcaga agaaagagaa ggacaagcct
     1741 gcgccgcggg gcacgcccgt gctgtggcgc gagcctgtgg acatcgcctc gcgcgacctg
     1801 ttcctggggc cgggcggcgc gtcgatgagg cccgacctgc gcggcgtcac cttcgtcaag
     1861 gaagagaagg gcggctactc gacgaagtac cgcgtgaagg acggcgcggg gcgcgtgtgg
     1921 gtggcgaaga tcgggaagga ggcgcagagc gagaccgccg ccgtgcggct gctgtgggcc
     1981 gtcggctacc cgaccgagat caactacctc gtgccgcgcc tcacgatcga aggcaagggc
     2041 acgttcgaga acgtgaggct cgaagcgcgg cccgaaagtg tggacaggct cgacgagtgg
     2101 aagtggagcg acaacccctt cgtcgggacg aaggagcttc aggggctgat cgtgttgatg
     2161 gctctcttga acaactggga catcaaggac gcgaacaaca aagtcttcca cacgcggggc
     2221 gagctgcgct acgtcatcag cgacctcggc gccaccttcg ggagggtcaa gtcgggcgcg
     2281 cccatcgtct ggcgcatccg ccgcaaccgc aacgaccccg cggactactc cgaagacccc
     2341 ttcgtcgaag acgtgaagcg cggcaacgtg ctgctcttct acaagggcaa gcggcaggac
     2401 ttgttcgatg acatccgcgt cgaggaggcg cgctggatcg gtggttggct cgcgcgcctg
     2461 agcgaccgcc agctcgccga cgccttccgc gccgccaact acacgcccgg cgaggtcaag
     2521 ctgctcgtcg gggccgtgcg cgcgaggatc aacgagctga cgagcctgcc cgcgggcggc
     2581 ctcgccaatc gccgctgaag acacctcgac acatcgctcg aagcgcgagc gccgcgtgac
     2641 gacttaggct tgcggcccga gttgaattat cattgcggcg ctcgccacat tttgccgcgc
     2701 gtgacctatc gtgctctgcg cgcaacgaac ggagaacagc cgaagtgagg aataaccaga
     2761 aagtcctcat cgtggcggcg ctcggcgccg ccgtcatcgg gttcggcatc gtcgccctcg
     2821 tcttcagggc gagggaggcg gaacggcccg cgcgcggcgt cgatacagtc ggcgtcctcg
     2881 ccagccccac gcagccggcg gcgactccgc cgcaacaaac gacgacggcg ccgaccgcga
     2941 caccgcgcgc gaaagacagc aacccgctcc tcgacctgct tcagggcgag gaggtgacga
     3001 ccaccgcgga gcaacaaccg cggggcgtcg tggagacgct gctgagcgtc acgctgcgcc
     3061 tgacgctggc cgccgtgctg gcggcgatgc tcgccttccg cccgcgccgc gtccgccgca
     3121 tcatccgccg caacccgttc gtcgcgcaga cgcagatcct gctcgcggtc gtctcctcgg
     3181 cgctgatgat gatcgtcggc gacagcgccg cgcgcgcctt cggcatcttc gccgccgtct
     3241 ccttcgtcag gttccgcacc aacatccgcg accccaaaga ggtgaccgtg ctgctcgtca
     3301 gcctcgccat cgggttggcg acgggcacgg gccgctggga gctggcgatc atcctcagct
     3361 tcttcatcct gctgctgctg tggggcctcg aataccgcga gccggagctg ctcttccgcg
     3421 cgatggaatt gaccgtgaag acgaagaacg tggacatgac gcaggaggtg ctgctccgcc
     3481 tcttccgcaa gtaccgcttc gacgccgaga tgcgcaccat cgaccggccc gacgccgagg
     3541 acgaggtggg ctgcgtcgtc taccacgtca acgtcagccc gctcatcagc accgaccggc
     3601 tcagcgaaga gatcttctcc gccgactccg ccaacatcga cagcgtcgag tgggatcagc
     3661 agaagaaccc cgcgcactac tatcagtagg cagcgggcag taggcggtaa aagcgagagg
     3721 cttgagtggt tgactctcaa gcctctcgct tcagtgctcg aagggcgtag aggctcggga
     3781 ctttccctcc caaacctctt cgcccttctg tcttgactgc cta
//
LOCUS       DBNGOH010000494         4755 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4654604, whole genome shotgun sequence.
ACCESSION   DBNGOH010000494 DBNGOH010000000
VERSION     DBNGOH010000494.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4755)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4755)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4755
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4654604"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..501)
                     /locus_tag="AC159P_09760"
     CDS             complement(<1..501)
                     /locus_tag="AC159P_09760"
                     /inference="COORDINATES: protein motif:HMM:NF024273.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HIR3695778.1"
                     /translation="MNRTKWVERRFDFSGVPVGVFPCVLERLRGTPARLEEMMRGLAE
                     DVLTARHDGAWSIKEQVGHLFDLDELHEGRLEDYDEGRETLRAADMENRKTYEANHNA
                     APVETLLASFRAARARFVARLEEMDEEAIARTALHPRLRQPMRVIDMALFVAEHDDHH
                     LAGVTEI"
     gene            complement(632..2581)
                     /locus_tag="AC159P_09765"
     CDS             complement(632..2581)
                     /locus_tag="AC159P_09765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695779.1"
                     /translation="MLRTRPFIDFAAALFLSLSSSYVLAQQPDRKPATPEKASAAAPA
                     EKPAADAPGEADLLAEARRTTPISLVTSLADEARSFRDHTLRARVQARAADALWDTEK
                     ERARLLFRRAWEAATAADDENERRLEEEKKRQERERGSFSIQLPPSLRTEVLRLAAKR
                     DRALGEEFLAEMDAAKKSATSATTPFDSDGDVDRNTSGAPRRNDPSEAPAAIAKRLRL
                     AIQLLEDGDTERAIQFADPALVGVTLPGLEFLARLRPKNAKAADERYAALVGRAALDP
                     ASDPNTASLLASYIFTPTLYVTFSPTGGSNSNSWGRGFPAPTDIAPQLRASYFGAASS
                     ILLRPVPPQDQDRTSTGRAGWFMVITRLLPLFDQFAPDRSAQLRAQLAQLLPDTPERS
                     RNPATNNSLTRGLVPEDPNRDRVQESLNRLANAKTADERDAIYVDAVLDATRQRDPRA
                     EEFVAKIENLELRRQLRAYVDYEAVERALRDKDAETALRIARAGNITSVQKTLALTEA
                     AKLYTKAEPGRAIELLDEALAEVKRIDQASPDRVRALVSVATQLSALDRPRAWEVMLD
                     IVKAANDAEGFTGEDGRITVRLQTKNMTNTSSSSVQSFDLAGIFTDLAREDLTRATSL
                     ARDFKGESPRAVATLAVARAVLDKK"
     gene            complement(2584..4551)
                     /locus_tag="AC159P_09770"
     CDS             complement(2584..4551)
                     /locus_tag="AC159P_09770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695780.1"
                     /translation="MSHPARLSAAALCLLTVPFPHAAAQQATPPAQTQKAPPATAAKK
                     KPAAADPLAEVRRQSAISLVVATADEANRSPDRILRARVQARAADVMWDADRTRAKMM
                     FRNAWRAAVFADEEAARLTDEERRRRETGRASTESRELPSMRREVLRLAARRDAALAQ
                     EFLAEMEGAPKPNPRGATASAADLSTLTPPAQTQYARINPDNPPPAMMQRLGLARQIL
                     EDGDADRAMQFADPALFPVNTYGMNILDMLREKNAAAADERFGRLLLLVANDPQADAN
                     TVSLLSSYVLTPYLYITVNREGASHTRRWRRENAPPADLPAPLRDSFLRTAAQILLRP
                     LPPADQDLSSAGRIGTYTIIARLAQVYERLLPDVAATLRARQSVLMQDVPENNRRPDD
                     PLLSRGLVPEAPSGDRVQEALDRLPNAKTADERDMIHYRAAMAAAETEPDRAREFVNK
                     IEDIELRRQLFAFLAFQAVERAIRDKRAEDVVRLSRGDELNSVQRTWALTEAARLFTK
                     DEPGRALEVLDDATNEARKIDQASPDRARALVAIVTQLHKLDRPRTWEVMNEVVKASN
                     SLPAFSGEDGGLTVRVRFKGGGAMTTNFNVESFDLAGVFDSLAREDFNRAADLSKALT
                     GEQPRAAAALAAARAVLDQRRRPGADEEEEN"
BASE COUNT          610 a         1504 c         1846 g          795 t
ORIGIN      
        1 tatctccgtg acgccggcga ggtggtggtc gtcgtgttcg gcgacgaaga gggccatgtc
       61 gatgacgcgc atcggctgcc ggaggcgcgg gtgcagggcg gtgcgggcga tggcctcctc
      121 gtccatctct tcgagccgcg cgacgaaacg cgcgcgcgcc gcgcggaacg atgccagcaa
      181 cgtctcgacc ggcgcggcgt tgtggttcgc ttcgtaggtc ttgcggttct ccatgtcggc
      241 ggcgcgcagg gtctcgcggc cttcgtcata atcttcgagg cggccctcgt gcagctcgtc
      301 cagatcgaag aggtggccga cctgctcctt gatcgaccac gcgccgtcgt gccgcgcggt
      361 gagcacgtcc tccgcgaggc cgcgcatcat ctcttcgagc cgcgcgggcg tgccgcgcag
      421 ccgctccaac acgcacggga agacgccgac gggcacgccg ctgaagtcga accggcgctc
      481 gacccacttc gttctgttca tcttcggttc gatacctccg cgtaaaaaat aaggcgaaaa
      541 agggaaaagg ggaagctgcg aagagggaag agcaagcaac tcgcctgtct ttcttttcgc
      601 ctcttcccct tttccccctc tgcttttccc cttacttttt atcgagcacg gcgcgggcga
      661 cggcgagcgt ggcgacggcg cggggcgact cgcctttgaa gtcgcgtgcg agcgaggtgg
      721 cgcgcgtgag gtcttcgcgg gcgaggtcgg tgaagatgcc cgcgaggtcg aagctctgga
      781 cgctgctgct cgacgtgttg gtcatgttct tggtttgcag acgcacggtg atgcgcccgt
      841 cctcgccggt gaagccttcg gcgtcgttgg ccgccttgac gatgtcgagc atgacctccc
      901 acgcgcgcgg gcggtcgagc gccgagagct gcgtggcgac ggagacgagc gcgcggacgc
      961 ggtcgggcga cgcctgatcg atgcgtttga cctcggcgag cgcttcgtcc aaaagctcga
     1021 tggcgcggcc cggctcggcc ttcgtgtaga gcttggccgc ctccgtgagc gccaacgtct
     1081 tctggacgga cgtgatattg ccggcgcgcg cgatacgcag cgcggtctcg gcgtccttgt
     1141 cgcgcagggc gcgctcgacg gcctcgtaat cgacgtaggc gcggagctgc cggcgcagct
     1201 caaggttctc gatcttggcc acgaactctt cggcgcgcgg gtctctctgg cgcgtcgcgt
     1261 cgagcacggc gtcaacgtag atggcgtcgc gctcgtccgc ggtcttcgcg ttcgcgaggc
     1321 ggttcaaaga ctcctgcacg cggtcgcggt tggggtcttc ggggaccagt ccgcgcgtga
     1381 gcgagttgtt ggtggcgggg ttgcgcgagc gctcgggcgt gtcgggcagg agctgcgcga
     1441 gctgcgcgcg gagctgcgcc gaacggtcgg gcgcgaactg gtcgaagagc gggagcagcc
     1501 gcgtgatgac catgaaccag ccggcgcggc ccgtcgaggt gcgatcctga tcctgcggcg
     1561 ggacggggcg cagcaggatt gacgacgcgg cgccgaaata ggaggcgcgg agctgcggcg
     1621 cgatgtcggt cggcgcgggg aagccgcggc cccaggagtt gctgttcgag ccgccggtcg
     1681 gcgagaaggt gacgtagagc gtgggcgtga agatgtagga cgcgaggagc gaggccgtgt
     1741 tcgggtcgga cgcggggtct aaggccgcgc gcccgacgag cgcggcgtag cgctcgtccg
     1801 ccgccttcgc gttcttgggc cgcaggcgcg cgaggaattc gaggccgggc aacgtgacgc
     1861 cgacgagcgc ggggtctgcg aactggatgg cgcgctcagt gtcgccgtct tcgagcagtt
     1921 ggatggcgag ccggaggcgc ttggcgatgg ccgcgggcgc ttcggacggg tcgttgcggc
     1981 ggggggcgcc gctcgtgttg cggtcaacgt cgccgtcgct gtcgaagggc gtggtggcgg
     2041 aagtggcgct cttcttcgcg gcgtccatct ccgcgaggaa ctcttcgccg agcgcgcggt
     2101 cgcgtttggc ggcgagccgg aggacttcgg tgcgcaggct cggcggcaac tggatggaga
     2161 acgagccgcg ctcgcgctcc tgccgtttct tttcttcttc gaggcgccgt tcgttctcgt
     2221 cgtccgcggc ggtggccgcc tcccacgcgc ggcggaagag caggcgcgct cgctccttct
     2281 ccgtgtccca cagggcgtct gcggcgcggg cctgcacgcg tgcgcgcagc gtgtggtcgc
     2341 ggaagctgcg ggcctcgtcg gcgagcgagg tgacgagcga gatgggggtc gtgcgccgcg
     2401 cctcggcgag caggtcggct tcgccgggcg cgtccgccgc gggcttctcg gcgggcgcgg
     2461 cggcggaagc cttctccggc gtcgcgggct tccggtcggg ctgctgcgcg aggacgtaag
     2521 acgacgacag cgacaggaag agcgcggcgg cgaagtcgat gaagggtcgc gtgcgaagca
     2581 tggtcagttc tcctcctctt cgtcggcgcc ggggcggcgg cgctggtcga gcacggcgcg
     2641 ggcggcggcg agcgcggcgg cggcgcgcgg ctgctcgccc gtgagcgcct tcgacagatc
     2701 ggcggcgcgg ttgaagtctt cgcgggcgag cgagtcgaag acgcccgcga ggtcgaacga
     2761 ctcgacgttg aagttggtcg tcatcgcgcc gccgcctttg aaccggacgc ggacggtgag
     2821 gccgccgtcc tcgccgctga aggcgggcag cgagttcgaa gccttgacca cctcgttcat
     2881 gacttcccac gtgcgcgggc ggtcgagctt gtgaagctgc gtgacgatag cgaccagtgc
     2941 gcgcgcgcgg tcgggcgacg cctgatcgat cttgcgcgct tcgttcgtgg cgtcgtcgag
     3001 cacttcgagc gcgcggcccg gctcgtcctt cgtgaacagc cgcgcggcct ccgtcagcgc
     3061 ccacgtgcgc tggacgctgt tcagctcgtc gccgcgcgag aggcgcacca cgtcctccgc
     3121 gcgcttgtcg cggatggcgc gctcgaccgc ctggaaggcg aggaaggcga agagctggcg
     3181 gcgcagctcg atgtcctcga tcttgttcac gaactcgcgc gcgcggtcgg gttcggtctc
     3241 ggcggcggcc atcgcggcgc ggtagtgaat catgtcgcgc tcgtcggcgg tcttggcgtt
     3301 gggcaggcgg tcgagtgcct cctgcacgcg gtcgccgctc ggcgcttcgg ggacgagtcc
     3361 gcgcgacaag agcgggtcgt cgggccgccg gttgttctcc ggcacgtcct gcatcagcac
     3421 ggactggcgc gcgcgcagcg tcgccgccac gtccggcagc agcctttcgt aaacctgcgc
     3481 gaggcgcgcg atgatcgtgt aggtgccgat gcgaccggcg gaggataagt cctggtcggc
     3541 gggcggcagc ggcctcagga ggatttgcgc cgccgtgcgg aggaacgagt cgcgcagggg
     3601 cgcgggcagg tccgcgggcg gcgcgttctc tcgccgccag cggcgcgtgt ggctcgcgcc
     3661 ctcgcggttg acggtgatgt agaggtacgg cgtgagcacg taggacgaga gcagcgagac
     3721 cgtgttcgcg tccgcctgcg ggtcgttcgc gacgagcagg agaagcctgc cgaagcgctc
     3781 gtcagccgcg gcggcgttct tttcgcgcag catgtcgagg atgttcatgc cgtaggtgtt
     3841 gacggggaag agagcggggt cggcgaactg catcgcgcgg tcggcgtcgc cgtcttcgag
     3901 aatctggcgg gcgaggccga gccgctgcat catcgcgggc ggagggttgt ccgggttgat
     3961 gcgcgcgtac tgggtctgcg cgggcggcgt cagggtggaa aggtcggcgg cgctcgcggt
     4021 cgcgcccctg gggttcggtt tgggcgcgcc ctccatctcg gcgaggaact cctgcgccaa
     4081 cgcggcgtcg cgccgcgccg ccagccgcag cacctcgcgg cgcatgctcg gcagctcgcg
     4141 cgactcggtg ctcgcgcgcc cagtctcgcg ccgccgccgc tcctcgtccg tgaggcgcgc
     4201 ggcctcctcg tcggcgaaga cggcggcgcg ccacgcgttg cggaacatca tcttcgcgcg
     4261 cgtccggtct gcgtcccaca tcacgtcggc ggcgcgcgcc tgcaccctcg cgcggagaat
     4321 gcggtcgggc gacctgttcg cctcgtccgc cgtggcgacg acgagcgaga tggccgactg
     4381 gcggcgcacc tcggcgagcg ggtcggcggc ggcgggcttc ttcttcgcgg cggtcgccgg
     4441 cggggctttc tgcgtctggg ccggaggcgt cgcctgctgc gcggcggcgt gcgggaaggg
     4501 aaccgtcaag aggcagaggg cggcggcgga aaggcgtgct ggatgagaca tacttcgctc
     4561 cttcggctgc gagcgcggct ggctcggcgg tgcgatgggc cgcggtctca aattgtgtgt
     4621 tcgactgacg ggcaagtgcg ggcgccgggc agagcgcggc ccgcgctctc gaatagatag
     4681 aaaggtaaac gtccgccggg gattacggtt ccggaaactt cagtggagcg acgcccgcag
     4741 cagttcaagg aagcg
//
LOCUS       DBNGOH010000495         2477 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_805948, whole genome shotgun sequence.
ACCESSION   DBNGOH010000495 DBNGOH010000000
VERSION     DBNGOH010000495.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2477)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2477)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2477
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_805948"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..246
                     /locus_tag="AC159P_09775"
     CDS             <1..246
                     /locus_tag="AC159P_09775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HIR3695781.1"
                     /translation="DGWLYGCDICQDVCPWNRFEQPSTEPRFEPRAGNVNADLSDILA
                     LTPDSYAARFRRSAVKRAKLAGLQRNARALSSRAGGE"
     gene            381..1745
                     /locus_tag="AC159P_09780"
     CDS             381..1745
                     /locus_tag="AC159P_09780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695782.1"
                     /translation="MHAPRRRTLRLAALLALTLLTTAAAAAFIRRALACPIEIPPQTL
                     RTLYKHSDRIVVARVGHTELVERDEDSSQVRTALHVARNVKGKDEGPVVFVHHSTWVE
                     EGVEKPGTYKQGEQLLVFLERGDEEEGRAVYTVDDATYGVKMLSDDDLKVYLARIDEL
                     AGIEGRKPADKAALVEWLVRCAEEPATRWEGAYELMVSTQALEHSKEQRERQPSEAEI
                     RASVPKHHATAEEYEADVARALAVAWGEAAVAPTSPAPPPGEAALAPPPGVAVEREPV
                     ELRRATPRDPSLAAALSEEQQRRLADALFGAEKIGVGEGALLHVVKGFQDARLVPFLV
                     AQLRRVEDNPPTEAEEWLAVLSEVSNNEEAVHLITNYSANVTYYEEEEEMTAGDESAG
                     TDKAAAVDEEESAGTDEEFDQKEQDARDAAATERATKKRSALLKDLLARVERILASPD
                     VAHQ"
     gene            2000..>2477
                     /locus_tag="AC159P_09785"
     CDS             2000..>2477
                     /locus_tag="AC159P_09785"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019586337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990140 - molybdopterin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0061798 - GTP 3',8'-cyclase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HIR3695783.1"
                     /translation="MTAVLKDSYGRAIRDLRVSVTDRCNFRCFYCLPHGEPPAAPKKE
                     MLTYEEIEYAVSVFASLGVEKVRLTGGEPMLRRDIETLVRKLSRTEGVRDLALTTNGF
                     NLPERAAGLKAAGLDRVTISLDSLRRDVFQRMTGVDVLDRVLEGVRAAKRVGLVPIK"
BASE COUNT          451 a          869 c          808 g          349 t
ORIGIN      
        1 gacggctggc tctacggctg cgacatctgt caggacgtgt gcccgtggaa ccgcttcgag
       61 cagccgagca cggaaccgcg cttcgagccg cgcgccggaa acgtcaacgc agacctctcc
      121 gacatcctcg ccctgacgcc cgactcttac gccgcccgct tccgccgcag cgccgtcaaa
      181 cgcgccaagc tcgcggggct gcaacgcaac gcgcgcgcgc tctcgtcgcg ggcgggcggc
      241 gaataacggg cgaacgcctt gaccttccac tcgacggaac cttttccggc gggccggtgt
      301 ctaagtattc agctccctat cgcgcaagtc cgcatcggcc tcgccgttct cggttcagct
      361 tcgccggggg aggtaagact atgcacgctc cgcgccgccg cacgctgcgg ctcgccgctc
      421 tgctcgcgct cacactactg acgaccgccg ccgccgccgc cttcatccgc cgggcgctcg
      481 cctgccctat cgagattccg ccgcagaccc tgcgcacgct ctacaaacac agcgaccgca
      541 tcgtcgtcgc gcgcgtcggc cacacggaac tcgtcgagcg cgacgaggac tcttcgcagg
      601 tgcgcaccgc gctccacgtc gcgcgcaacg tgaagggcaa ggacgaaggg ccggtggtct
      661 tcgtccacca ctcgacgtgg gtcgaagaag gggtcgagaa gccgggcacc tacaagcagg
      721 gcgagcagtt gctcgtcttc ctcgaacgcg gcgacgaaga ggaaggccgc gcagtctaca
      781 cggttgacga cgcgacctac ggcgtcaaga tgctctcgga cgacgacctg aaagtctacc
      841 tcgcgcgcat cgacgagctg gccggcatcg agggccgaaa gcccgcggac aaggcggcgc
      901 tcgtcgaatg gctcgtgcgc tgcgccgagg aacccgcgac gcgctgggag ggcgcctacg
      961 agttgatggt gagcacgcag gcgctggagc attcgaagga gcagagagag agacagccga
     1021 gcgaggcgga gatcagagcg tccgtcccga agcaccacgc cacggcggag gagtacgaag
     1081 ccgacgtcgc gcgcgctctg gcggtggcgt ggggcgaggc cgccgtcgcg cccacgtccc
     1141 ccgccccgcc gcccggcgaa gccgctctcg caccgccgcc gggcgtcgcc gtcgagagag
     1201 agcctgtcga gttgcgcagg gccacgccgc gcgacccgtc gctcgcggcg gccttgagcg
     1261 aagagcagca gcggcggctg gccgacgcgc tcttcggcgc cgagaagatc ggcgtgggcg
     1321 agggcgcact gctccacgtc gtcaagggct tccaagacgc gcgcctcgtc cccttcctcg
     1381 tcgcgcaact gcgccgcgtc gaagacaacc cgccgaccga agccgaggag tggctcgcgg
     1441 tgctctcgga agtgtcgaat aacgaagagg ccgtccatct catcacgaac tactccgcga
     1501 acgtcaccta ctacgaagaa gaagaggaaa tgacggcggg cgacgaatcc gccgggacgg
     1561 acaaagcggc ggcggttgac gaggaggagt cagccgggac ggacgaagag ttcgaccaga
     1621 aagagcagga cgcccgcgac gccgccgcca ccgagcgcgc caccaaaaag cggagcgcac
     1681 tgctcaaaga cctcctcgct cgcgtcgagc gcatcctcgc ctcgcccgat gtcgcgcacc
     1741 aatgagcgaa gcacgttcac acccgaaaac ggaatagccg cggatgagcg cggatgaaac
     1801 tcggatcgga aatgattttc gaagctgctc cgaaccgcgt ttcatccgcg tttcatccgc
     1861 ggctattcct gttccgcttt gcgaacagtc atcacccgcc cgccggtttg gtgcgcgcgt
     1921 ccgcgcgtcg tatcataacg cttgccgctt cgcctcttgg ttgaagccgt gggcgaacgg
     1981 cgagcgagtg aaacccgaag tgactgccgt actcaaagac tcatacggtc gcgccatccg
     2041 agacctgcgc gtgtcggtga cggacaggtg caacttccgc tgcttctact gcctgccgca
     2101 cggcgagccg cccgccgcgc cgaagaaaga gatgctcacc tacgaggaga tcgagtacgc
     2161 cgtctcggtc ttcgcctcgc tcggcgtcga gaaggtgcgc ctcacgggcg gcgagccgat
     2221 gctccgccgc gacatcgaga cgctcgtccg aaagctctcg cgcacggaag gcgtccgcga
     2281 cctcgcgctc acgaccaacg gcttcaacct gcccgagcgc gccgccggcc ttaaggccgc
     2341 cggactcgac cgcgtgacga tcagcctcga cagcctgcgc cgcgatgtct tccagcggat
     2401 gacgggcgtg gacgtgctcg accgcgtgct cgaaggcgtg cgcgcggcga agcgcgtcgg
     2461 ccttgtgccg atcaagg
//
LOCUS       DBNGOH010000496         1723 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2457028, whole genome shotgun sequence.
ACCESSION   DBNGOH010000496 DBNGOH010000000
VERSION     DBNGOH010000496.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1723)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1723)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1723
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2457028"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(77..745)
                     /locus_tag="AC159P_09790"
     CDS             complement(77..745)
                     /locus_tag="AC159P_09790"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="PEP-CTERM proteins occur, often in large numbers,
                     in the proteomes of bacteria that also encode an
                     exosortase, a predicted intramembrane cysteine proteinase.
                     The presence of a PEP-CTERM domain at a protein's
                     C-terminus predicts cleavage within the sorting domain,
                     followed by covalent anchoring to some some component of
                     the (usually Gram-negative) cell surface. Many PEP-CTERM
                     proteins exhibit an unusual sequence composition that
                     includes large numbers of potential glycosylation sites.
                     Expression of one such protein has been shown restore the
                     ability of a bacterium to form floc, a type of biofilm;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HIR3695784.1"
                     /translation="MTRLLISTIFVLASAAGARADIITFFDFNDSDLVSDRGQSATIT
                     TTFGALGFATGSTVGADPDGAGPETPSAAGQALNLVGSANNGNHIQFGVSTLGLVPTQ
                     LSFATQRSSTGFNNNELLFSLDGGQSFISVGFFTPPTTFALQTFNLASLAALQNQPEV
                     VFRILLNGASSATGTNRIDNLLVTGDPLSTPVPEPMSVLLLGAGLLGLARTVRRRSRD
                     GGHR"
     gene            complement(1010..>1723)
                     /gene="alr"
                     /locus_tag="AC159P_09795"
     CDS             complement(1010..>1723)
                     /gene="alr"
                     /locus_tag="AC159P_09795"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HIR3695785.1"
                     /translation="EFAEALRALRHVRVDGLMTHFAAADEPGRDCFTREQLGRFEEAK
                     EIFRARGHAPRFEHLANSAATFAHPETRGNMVRPGGVLYGLWRDVLPAGAEAPSMRPV
                     MALRSRVTLLKRVRRGETLGYGCTYEAAREMRVATVPVGYADGYPRALSNRGRVIVRG
                     TYAPVVGRVSMDLTLVDVTDIEGVETGERVTLLGADGELTIPAEDLAKTVGTISYEIT
                     CGVSSRVPRRYAPVDKTGV"
BASE COUNT          279 a          584 c          598 g          262 t
ORIGIN      
        1 cgcccctccg cgtactctgc gtcgaatcct gcccacggta agctcaatcg caagctctaa
       61 tccgacgcgc cgcacgtcaa cggtggccgc catcccgact gcggcggcgc acggtgcggg
      121 ccaagcccaa cagccccgcg ccgagtaaga gcacggacat cggctccggc acaggcgtcg
      181 ataggggatc gcccgtgacc agcaggttgt cgatccggtt cgtccccgtc gcgctcgacg
      241 ccccgttgag caggatgcgg aagaccacct ccggctggtt ctggagcgcg gcgagcgagg
      301 cgaggttgaa ggtttgcagc gcgaaggtcg tcggcggggt gaagaagccg accgagatga
      361 agctctgacc gccgtcgagg ctgaacagca gctcgttgtt gttgaagccc gtgctgctcc
      421 gctgcgtggc gaacgaaagc tgcgtcggga cgaggcccag cgtgctcacg ccgaactgga
      481 tgtggttgcc gttgttggcg ctgcccacga ggttgagcgc ctgtcccgcc gccgaaggcg
      541 tctccgggcc ggcgccgtcc gggtcggcgc ccaccgtgct gccggtcgcg aagccgagcg
      601 cgccgaaagt ggtcgtgatg gtcgcgctct gcccgcggtc ggagacgagg tcggagtcgt
      661 tgaagtcgaa gaaggtgatg atgtcggccc gcgcgcccgc ggctgacgcc aacacgaaga
      721 tcgtgctgat gaggagtctg gtcattggat ttggcctctt tggttttcgc acgtgggggg
      781 tcgcgcagcc gcccgccggc gcttccgaga gaggcgccgt ggcgaccgcc agtgcggtca
      841 tgcccttgac cggaaagact tccagccgga acgaggcgcg agacaacaca cgggcggtcg
      901 tcgcctgtcg tgcacggcgg tgtcagtgtt caggtgaaat acatggcctc acgttgggcg
      961 ggatgaggac gcgggccgtt cgtaaccaat gcgccggaag taaatacagc taaacgcccg
     1021 ttttgtcaac gggggcgtac cgccgcggca cgcgcgagct gacgccgcag gtgatttcgt
     1081 aggagatggt gccgaccgtc ttcgccaaat cttcggcggg aatcgtgagt tcgccgtcgg
     1141 cgccgagcag cgtcacgcgc tcgcccgtct cgacgccctc gatgtccgtc acgtcaacca
     1201 acgtcaaatc catcgagacg cggccgacga ccggcgcgta cgtcccgcgc acgatgacgc
     1261 gcccgcggtt cgacagcgcg cgcgggtagc cgtccgcgta gccgacgggc acggtcgcca
     1321 cacgcatctc gcgcgccgcc tcgtaggtgc agccgtagcc cagcgtctcg ccgcgccgca
     1381 cgcgcttcag gagcgtcacg cgcgagcgca acgccatcac gggccgcatg gacggcgcct
     1441 cggccccggc gggcagcaca tcgcgccaca gaccgtagag cacgccgccc ggcctcacca
     1501 tgttgccgcg cgtctcgggg tgcgcgaacg tggcggcgga gttcgccaga tgctcgaagc
     1561 gcggcgcgtg gccgcgcgcg cggaaaatct ccttcgcctc ctcgaagcgc cctaactgct
     1621 cgcgcgtgaa gcagtcgcgg cccggctcgt ccgccgccgc gaagtgcgtc atcagaccgt
     1681 caacgcggac gtgccgcaac gcgcgcaagg cttcggcgaa ctc
//
LOCUS       DBNGOH010000497         3815 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3739346, whole genome shotgun sequence.
ACCESSION   DBNGOH010000497 DBNGOH010000000
VERSION     DBNGOH010000497.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3815)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3815)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3815
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3739346"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..947)
                     /locus_tag="AC159P_09800"
     CDS             complement(<1..947)
                     /locus_tag="AC159P_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013937881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HIR3695786.1"
                     /translation="MPEAEMESMPEVTRYFHPVLASKRLKKEPVRVEVAGRAYVLFRD
                     AEGRAAALVDRCPHRFAPLSKGRVRADGRLACGYHGWNFDAEGCGQSPSQPSLTKCDV
                     RSFRTVERFGYVWMAERDVPLASFPSMEWEGYEYAGAFDTVFEAPIHVSLANFSEDEH
                     FPYVHTRLGWDDRRLEEIHFEADNYEDRSEVRYRGPQRPSPYLKLLLVKPGDRFYNEW
                     VTRFDPLRALFSFRWEDPRTGRERPLKLKTMIFMTPETERTTRFHVFLFLRLDAPRLR
                     FLLPVARLAALKMGRHEVRDDANFARHLADTPSDMKGMRL"
     gene            complement(1019..2467)
                     /locus_tag="AC159P_09805"
     CDS             complement(1019..2467)
                     /locus_tag="AC159P_09805"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     NtrB"
                     /protein_id="HIR3695787.1"
                     /translation="MAGRYENRMQLALALVVGLLLLANLFTLTLVAMSPATRAARAPV
                     LFTVFLLTLVVSVPCILLLPRWLLRPYRQLVDEAERAPVATSARGKARDETEFVLETF
                     QDVVAQLHAKQRELERLSLLASRRAESAEEFSERIVASVPSGLVAFDAAGRATVVNEP
                     ARTLLDCDGAAVGQDVRKLLGGAPELASMVESCLRSAELYRREEVAATGAGGRARRLG
                     VTVAPIDPQPGAGARGALCLLTDITEVVQLREAVALKRNLESLGEMAAGLAHEFKNAL
                     AALHGYTQLLQTRTLDDAARAASSAALLEEVRNLSEMVTAFLNFARPKPLDVSDVSLG
                     ELCEQCAADLAGFYEERGVALSLEGDFPVVRADERMLRQALLNLLRNAAEAIGEEGGA
                     RRVFVRGSVERDEGDATWAAVEIADTGDGIADEDLHKIFIPFFTTKSKGHGVGLALAH
                     RVVTDHGGTLSAANAHGGGAVFTLRLPAAVRA"
     gene            complement(2609..>3815)
                     /locus_tag="AC159P_09810"
     CDS             complement(2609..>3815)
                     /locus_tag="AC159P_09810"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014081.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR3695788.1"
                     /translation="ANAQSQAKGGRIVIAASAVLDGRGRVLRDTRIVVEGLKIVAVDP
                     KAGPVTYDLRGLTVLPGWIDAHVHITWSFGADGKNAGAGGTTQEAAYASASNAWATLL
                     AGFTTVQSVGSPADVPLRDAIARGALPGPRVLTAAEPLTGRGEQTGAPDEIRAYVRKQ
                     KEAGADVIKIYAAGGMRRGAMTMSQEQLSAACDEARKRGLRTLVHAYREAVRAATLAG
                     CTQVEHGLGATDEDLKLLAERGTYFDPQGGLLLKNYLLHKEKYLGTPFYPEESFAAFE
                     QVLPLNRELMRRAAKTPGLKVVFGSDAVAGAHGRNAEEFIDRVRAGGVDAMAALVSAN
                     SLGAEALGMADRLGSIAPGMLADIIALDGDPLKDITAVRRVVFVMKGGGVYKNAARAA
                     MPVNSGVRH"
BASE COUNT          579 a         1330 c         1308 g          598 t
ORIGIN      
        1 aggcgcatgc ccttcatgtc ggagggcgtg tcggcgaggt ggcgtgcgaa gttggcgtcg
       61 tcgcgcactt cgtgccggcc catcttgagc gcggcgaggc gtgcgacagg cagcaggaag
      121 cgcaggcgcg gggcgtcgag gcggaggaag aggaagacgt ggaagcgcgt cgtcctttcg
      181 gtctccggcg tcatgaagat catggtcttg agcttgagcg ggcgctcgcg gcccgtccgc
      241 gggtcttccc acctgaaaga gaagagcgcg cgcaaagggt cgaagcgcgt gacccactcg
      301 ttataaaagc ggtcgccggg tttgacgagc agaagcttca gatatggcga ggggcgctgc
      361 gggccgcggt agcggacttc ggagcggtct tcgtagttgt cggcctcgaa gtgaatctct
      421 tccaaacggc ggtcgtccca gccgagccgc gtgtggacgt aggggaagtg ctcgtcctcg
      481 ctgaagttgg cgagcgagac gtgaatcggc gcctcgaaca ccgtgtcgaa ggcgcccgcg
      541 tattcgtagc cctcccactc catcgaaggg aacgaggcga gcggcacgtc gcgctcggcc
      601 atccagacgt agccgaatcg ctcgaccgtc cggaacgagc gcacgtcgca cttcgtcagc
      661 gaaggctgcg aagggctttg gccgcagccc tcggcgtcga agttccagcc gtggtagccg
      721 cacgcgagcc gaccgtccgc gcgcacgcgc cccttcgaga gcggcgcgaa acggtgcggg
      781 catctatcga cgagcgccgc ggccctccct tccgcgtcgc ggaagaggac gtacgcgcgg
      841 cccgcgacct cgacacgcac cggctccttc ttcaaacgct tcgacgcgag gacggggtga
      901 aagtatcgcg tgacttcggg catcgactcc atttcggcct ccggcaaacc gcgtagactg
      961 cgcggccatt atttcataac tctggtacgc cgggagaggc ggattcagat tccgcgactc
     1021 aggctcgaac cgccgccggg agccgcagcg tgaagaccgc gccgccgccg tgcgcgttgg
     1081 ccgccgagag cgtgccgccg tgatccgtga cgacgcggtg ggcgagcgcg aggccgacgc
     1141 cgtggccctt cgacttggtg gtgaagaagg ggatgaagat tttgtgcagg tcttcgtcgg
     1201 cgatgccgtc gccggtgtcg gcgatctcga cggcggccca cgtggcgtcg ccttcgtcgc
     1261 gctcgacgct gccgcggaca aagacgcggc gcgcgccgcc ctcttcgccg atggcctccg
     1321 ccgcgttccg caggaggttg agcagcgcct gccgcagcat ccgctcgtcg gcccggacga
     1381 ccgggaagtc gccttcgagc gagagcgcga cgccgcgctc ttcgtagaag cccgcgaggt
     1441 ctgcggcgca ctgctcgcac agttcgccga gcgacacgtc ggagacatcg agcggcttcg
     1501 ggcgcgcgaa gttgaggaag gccgtcacca tctcggagag attgcgcacc tcttcgagca
     1561 gcgcggcgga agaggcggca cgcgccgcgt cgtcgagcgt gcgcgtctgc aagagctgcg
     1621 tgtagccgtg gagcgcggcg agcgcgttct tgaattcgtg cgcgaggccg gcggccatct
     1681 cgccgaggct ttcgaggttg cgcttgagcg cgacggcctc gcgcaactgc acgacctccg
     1741 tgatgtccgt gagcaggcag agcgcgccgc gcgcgcccgc gcccggctgc gggtcgatgg
     1801 gggcgaccgt gacgcccagc ctgcgcgcgc gcccgcccgc gcccgtcgcg gcgacctctt
     1861 cgcggcggta gagttccgcg ctccggagac agctctcgac catcgaggcc agctcgggcg
     1921 ccccgccgag cagcttgcgc acgtcctgcc cgacggcggc gccgtcgcag tcgaggagcg
     1981 tgcgcgccgg ctcgttgacg accgtggcgc ggcccgccgc gtcgaaggcg acgaggcccg
     2041 aagggacgct cgccacgatg cgctcgctga actcttcggc ggactcggcg cggcggctgg
     2101 cgagcaggct cagccgctca agctcgcgct gcttggcgtg gagctgcgcc accacgtcct
     2161 gaaaagtttc gagcacgaac tcggtctcgt cgcgtgcctt cccgcgcgcg gaagtcgcga
     2221 ccggcgcccg ctcggcttca tcgacgagct gccggtacgg cctcagcagc cagcgcggca
     2281 acagcaggat gcacggcacg ctcacgacga gcgtgagaag gaagaccgtg aacagcacgg
     2341 gcgcccgcgc ggcgcgcgtc gcgggcgaca tcgccaccag cgtcagcgtg aagaggttgg
     2401 cgagcagcag cagccccacc accagcgcca gcgccaactg catccggttc tcgtaacgtc
     2461 cagccatact cgacgcaagg cgattatttg gaggggtagg gcttgtccct gcccgcgttg
     2521 acattccatc caacgtcgcc ttcatcaaat cgtgacgggc agggacaagc cctgcccctc
     2581 caattatccc cggtcttgga tagttgctct aatgccggac gcccgaattg acgggcatcg
     2641 cggcgcgcgc ggcgttcttg tagacgccgc cgcccttcat cacgaagacg acgcgccgga
     2701 cggctgtgat gtctttcaaa gggtcgccgt cgagcgcgat gatgtccgcc aacatgcccg
     2761 gcgcgatgga gcctagccgg tcggccatgc cgagcgcttc cgcccccagc gagttggccg
     2821 agaccagcgc cgccatcgcg tccacgccgc cggcgcgcac gcggtcgatg aactcttcgg
     2881 cgttgcgccc gtgcgcgccc gccacggcat ccgagccgaa gacgaccttc agccccggcg
     2941 tcttcgccgc ccgccgcatc agctcccggt tcaacggcag gacttgctcg aacgccgcga
     3001 aagactcttc ggggtagaag ggcgtgccga gatacttctc cttgtgcagg agataatttt
     3061 tgagcagcag cccgccctgc ggatcgaaat acgtccctct ttcggccagc agcttcaggt
     3121 cttcgtccgt cgcccccagc ccgtgctcga cctgcgtgca tccggcgaga gtcgcggcgc
     3181 ggacggcttc tctgtaagcg tgcacgagcg tgcgcagtcc ccgcttccgc gcctcgtcgc
     3241 aggccgcgct cagttgctcc tgcgacatcg tcatcgcgcc ccggcgcatg ccgcccgccg
     3301 catagatttt aatcacgtcg gcgcccgcct ctttctgctt cctgacgtac gcgcggattt
     3361 cgtcgggcgc cccggtctgc tcgcctcgcc ccgtgagcgg ctcggcggcg gtcaggacgc
     3421 gcgggccggg gagcgcgccg cgcgcgatgg cgtcgcgcag gggaacgtcc gccggcgagc
     3481 cgacgctctg caccgtcgtg aagccggcca gcagcgtcgc ccacgcgttc gaggccgaag
     3541 cgtaagcggc ctcctgcgtc gtcccgcccg cccccgcgtt cttcccgtcc gcgccgaagc
     3601 tccaggtgat gtggacatgc gcgtcgatcc agccgggcag cacggtcagg ccgcgcaggt
     3661 cgtaagttac cggccccgcc ttcgggtcaa cggcgacgat cttcaagccc tcgaccacga
     3721 ttcgcgtgtc gcgcagcacg cggcctctgc cgtcgagcac ggcgctcgcg gcgatcacga
     3781 tgcgcccgcc cttcgcttgc gactgcgcgt tcgcc
//
LOCUS       DBNGOH010000498         2544 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3555814, whole genome shotgun sequence.
ACCESSION   DBNGOH010000498 DBNGOH010000000
VERSION     DBNGOH010000498.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2544)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2544)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2544
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3555814"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(57..989)
                     /locus_tag="AC159P_09815"
     CDS             complement(57..989)
                     /locus_tag="AC159P_09815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695789.1"
                     /translation="MALRPMFEHAPGGWLAFELSILRRLQFSSVVNPFAGEPDLDVYL
                     KRWGARVSVNDSAQWAWVRGLARVENNTERLTEEDVEVVLEDAYVPRHRLYNPSLRRW
                     FGETDAWWFDNVRANADKLTDERRRALALHFGMMVGDYVHSFDEETRELRQPLSRVFR
                     RLWESDAAPVDNQARNTSSNKDARVFIANESADLLFLRLPHPSRRGPRHSAWAWREEW
                     VRGADDFWDDFEAAREGRLGARAETRQQYLRHVEELLAAATHFPACAVAHVENGLVST
                     DELVEVVRRTRRVGTVYTKDFSELTGARAVIITA"
     gene            complement(1075..1857)
                     /locus_tag="AC159P_09820"
     CDS             complement(1075..1857)
                     /locus_tag="AC159P_09820"
                     /inference="COORDINATES: protein motif:HMM:NF024878.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004112 - cyclic-nucleotide
                     phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HIR3695790.1"
                     /translation="MEFELAENDRAAGEAFMRLGAAVDEFEGYARPHALRVAKLSDAL
                     AARFRLSRADRASLVFAALLHDLGEMTMKRDYIQRAGPLSGAERLDLARHPVIGEQEA
                     ARAGADRGAQLLVRWHHEWWNGAGYPDALRAEQIPLEARILRVADSYAALTDARPFRP
                     AGTEAEARKHLMLWAGLEFDPHVVQVFLSLSGLAELRSYARAAAPPEPQRETTAIEVG
                     SNGEGVANVDEEAAVKNEEAVMKDEGTAVAEVASENPWNPRT"
     gene            complement(2019..>2544)
                     /locus_tag="AC159P_09825"
     CDS             complement(2019..>2544)
                     /locus_tag="AC159P_09825"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3695791.1"
                     /translation="TSDSTPSIRRASASDASLLAELGARTFYDAFIADNTAEDMDAYM
                     SVAFTPERLAAELAAANNAFFVAEIEDAPVGYAQLRAGEVPSCVEGARPVELARLYVS
                     QAWLGRGVGEALMQACVEEACAAGHRTIWLGVWERNLRAQAFYRKWDFRVVGDHVFVL
                     GSDPQTDLLMERAV"
BASE COUNT          391 a          820 c          904 g          429 t
ORIGIN      
        1 gagtcctccg tgccctccgt gttgaacctt gcgctcggta aacccgctcg ccgacttcag
       61 gccgtgatga tgacggcgcg cgcgcccgtc agctcggaga agtctttggt gtagacggtg
      121 ccgacgcggc gcgtgcggcg gacgacttcg acgagttcgt ccgtcgagac gaggccgttc
      181 tcgacgtggg cgacggcgca ggcggggaag tgcgtggcgg cggcgagcag ttcttcgacg
      241 tggcgcaggt actgctggcg cgtctcggcg cgggcgccga ggcggccctc gcgcgcggcc
      301 tcgaagtcgt cccagaagtc gtccgcgccg cgcacccact cttcgcgcca ggcccatgcc
      361 gagtggcgcg ggccgcggcg cgaggggtgc ggcaggcgca ggaacagcag gtcggcggac
      421 tcgttcgcga tgaagacgcg cgcgtccttg ttcgagctgg tgttgcgcgc ttggttatcg
      481 acgggcgcgg cgtccgactc ccacaggcgg cggaagacgc gcgagagcgg ctggcgcagc
      541 tcgcgcgtct cctcgtcgaa cgagtggacg tagtcgccga ccatcatccc gaagtggagc
      601 gcgagcgcgc gccgccgctc gtccgtgagc ttgtcggcgt tggcgcgcac gttgtcgaac
      661 caccacgcgt ccgtttcgcc gaaccaccgc cgcaagctcg ggttgtacag cctgtggcgc
      721 gggacgtagg cgtcttccag cacgacctcc acgtcctctt ccgtcagccg ctcggtgttg
      781 ttctcgacgc gcgcgaggcc ccgcacccac gcccactgcg ccgagtcgtt gaccgagaca
      841 cgcgcgcccc agcgcttgag atacacgtcg aggtccggct cgcccgcgaa ggggttgacg
      901 accgagctga attgcagacg gcgcaggatg ctcagctcga aggcgagcca gccgccgggg
      961 gcgtgttcga acatggggcg caaggccatc ggtctcctat gactatctca aagtactgct
     1021 gagtactgag gataaagaca attgttttta cctcagtact cagtacttta gtagttatgt
     1081 tcttgggttc cacggattct ccgaagcgac ttcggcgacc gccgtccctt cgtccttcat
     1141 gaccgcttct tcgtttttga cagcggcttc ttcatcaacg tttgcgacgc cttcgccgtt
     1201 gctgccgact tcgattgcgg tcgtctcgcg ctggggttcg ggcggcgcgg cggcgcgcgc
     1261 gtaggagcgc agctccgcta gccccgagag cgagaggaag acctgtacga cgtgcgggtc
     1321 gaactcaagg ccggcccaga gcatgaggtg cttgcgcgcc tcggcttcgg tgccggcggg
     1381 gcggaagggg cgcgcgtccg tgagcgcggc gtaggagtcg gcgacgcgca ggatgcgtgc
     1441 ttcgagcgga atctgttcgg cgcgcagcgc gtccgggtag cccgcgccgt tccaccactc
     1501 gtggtgccag cggacgagga gttgcgcgcc gcggtcggcg cctgcgcgcg cggcctcctg
     1561 ctcgccgatg acggggtggc gcgcgaggtc gagccgctcg gcgccgctga gcggcccggc
     1621 gcgctggatg tagtcgcgct tcatcgtcat ctcgccgagg tcgtgcagga gcgcggcgaa
     1681 gacgagcgag gcgcggtcgg cgcgcgacag gcggaagcgc gcggccagcg cgtccgagag
     1741 cttcgccacg cgcagcgcgt gcgggcgcgc gtagccctcg aactcgtcca ccgccgcccc
     1801 cagacgcatg aacgcctcgc ccgccgcgcg gtcgttctcc gctaactcga attccatagg
     1861 gttcgaagtt taaagttcaa agttgaaagt cgcaagcggg cggaaaagcg gaggggaaaa
     1921 ggttaaaagg ggaaaagggg aagagggaaa agataacggg cggcttgcct gcctttatcc
     1981 cttttcgcca tttcccctta ttaacctttt cccctctttc atacagcgcg ctccatcagc
     2041 aggtcggtct gcgggtcgga gccgaggacg aagacatggt cgccgacgac gcggaagtcc
     2101 cacttgcggt agaaggcttg cgcgcgcagg ttgcgctccc agacgccgag ccagatcgtc
     2161 cggtggccgg cggcgcacgc ctcttcgacg caggcttgca tgagcgcctc gccgacgccg
     2221 cgaccgagcc acgcctgcga aacgtagagg cgggccagct cgaccggcct cgcgccctcg
     2281 acgcacgaag ggacttcgcc cgcgcgcagt tgcgcgtagc cgaccggggc gtcttcgatc
     2341 tcggcgacga agaaggcgtt gttcgcggcg gccagctcgg cggccagcct ctcgggcgtg
     2401 aaggcgacgg acatgtacgc gtccatgtct tccgccgtgt tgtcggcgat gaaggcgtcg
     2461 tagaaagtgc gcgcgccgag ttcggcgagc agcgacgcgt ccgatgccga ggcgcggcgg
     2521 atggaggggg tgctgtcaga agtc
//
LOCUS       DBNGOH010000499         3327 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4655166, whole genome shotgun sequence.
ACCESSION   DBNGOH010000499 DBNGOH010000000
VERSION     DBNGOH010000499.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3327)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3327)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3327
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4655166"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..308
                     /locus_tag="AC159P_09830"
     CDS             <1..308
                     /locus_tag="AC159P_09830"
                     /inference="COORDINATES: protein motif:HMM:NF040068.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HIR3695792.1"
                     /translation="LMGGAASFLNPEPIAHFTDAVAVGEGEILVPRLADLIFENAEEG
                     GTKEDLLLKLARVGRGFYVPSLYEVTYNADGTVEAYTPKAEGVPERVGRMVAAENPK"
     gene            315..1484
                     /locus_tag="AC159P_09835"
     CDS             315..1484
                     /locus_tag="AC159P_09835"
                     /inference="COORDINATES: protein motif:HMM:NF015983.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HIR3695793.1"
                     /translation="MRRAIRRGDAEIVAKLKEPEVFAPSTVIYAPDAEMGERFLVEIS
                     RGCSQGCRFCWAGFNYWPPRVVPARDILAKAEVWRARTDKIGLVSTAVCDHPEISEIL
                     QGLRRMGFKISVSSLRLDQIEDELLDVLVESGDQQIAVAPETGSDRLRKVINKNLTND
                     EIVDICGAVFDRGMLTVKLYLMAGLPTERDEDLEEMVVLVERIKDRMLEAGRRFGRAG
                     KIIPALNGFVPKPNTPFQWEPICEERELKRRLKWLSKNLARIPNVEVRVMSARIAHEQ
                     ALFSSGDRSAARVIEAMTRHRDNLDAALRETGVDIAFHTRRARPYAEVLPWEIVDAGL
                     GREFMEKEHTRAQIARSTAPCPSVDTCTRCGVCPTTWLAAAPAGLVQLKGVAAGA"
     gene            complement(1588..>3327)
                     /locus_tag="AC159P_09840"
     CDS             complement(1588..>3327)
                     /locus_tag="AC159P_09840"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015175043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HIR3695794.1"
                     /translation="VVKYVTNTSAVAVAQALKTDQPLWQTWTDNYLWTSITYFAGAFA
                     AAIIVKMGNAVGYYAVVAVAPVVAIIYWTYRTYTKNIEMAAAAAKAEQAERHVEELNR
                     HISEQHRISRELEESREHFRRAAFHDALTGLPNRSLLTDQLREAIRRTVEQDEYRYAV
                     LFLDMDRFKNINDSLGHVAGDRFLVMIARRLERCVRPGDMVARLGGDEFAILLSDLGD
                     FHDAVRVAERVQKELTRPFNLNGHDVYTTASIGITLSTIGYEHPENILRDADTAMYRA
                     KENGKARHEVFDTVMHARAVALLQLESDLRRAVERGELRVYYQPIVNIETERIAGFEA
                     LARWQHPERGLVSPAEFIPVAEDTGLIVEIGQWVLEESCRQMREWQKLSPVFRPLTVS
                     VNLSSKQFAQPDLIDRIKRTLQETDLDPRCLKLEITESVVMKSAETASSMLKQLRALG
                     VHVGIDDFGTGYSSLSYLHRFPVNTLKIDRFFIGKMGAGDENSEIVRTIITLASNLGM
                     EVVAEGIETAEQLAQLRALKCEYGQGFFFSRPLDGRKAEALLISWSETEVAACYAADE
                     QLAGDATDFRFLN"
BASE COUNT          534 a          979 c         1228 g          586 t
ORIGIN      
        1 tgctgatggg cggcgcggca tcgttcctca acccggaacc catcgcgcac ttcacggacg
       61 cggtcgccgt cggcgagggc gagattctcg tcccccgact cgccgacttg atcttcgaga
      121 acgcggagga gggcgggacg aaggaagacc tgctcctgaa gctcgcgcgc gtcgggagag
      181 gcttctacgt gccttcgctg tacgaggtga cgtacaacgc ggacggcacg gtcgaggctt
      241 acacgccgaa ggcggagggt gtgcccgagc gcgtcgggag gatggtcgcg gcggagaacc
      301 cgaagtaggg gagtttgcgt cgcgccatca ggcgcggcga cgcggagatc gtggcgaagc
      361 tcaaagagcc ggaggtgttc gcgccttcga ccgtcatcta cgcgcccgat gcggagatgg
      421 gcgagcgctt cctcgtcgag atttcgcgcg gctgctcgca ggggtgccgc ttctgctggg
      481 cgggcttcaa ctactggccg ccgcgcgtcg tgcccgcgcg cgacattctg gcgaaggccg
      541 aagtgtggcg cgcgcgcacg gacaagatcg gattggtttc gaccgccgtc tgcgaccacc
      601 cggagatcag cgaaatcttg cagggcctga gacgcatggg gttcaagatt tcggtgtcgt
      661 cgttaaggct cgaccagatc gaggacgagc tgctggacgt gctcgtcgag tcgggcgacc
      721 agcagatcgc ggtcgcgccg gagaccggct ccgaccgtct gcgtaaagtc atcaacaaga
      781 atttgacgaa cgacgagatc gtggacatct gtggcgcggt gttcgaccgc gggatgctga
      841 ccgtcaagct ctatctgatg gcggggctgc cgaccgaacg ggacgaagat ttggaagaga
      901 tggtcgtgct cgtcgagcgg atcaaagacc ggatgctcga agccgggcgg cggttcgggc
      961 gcgcgggcaa aatcatcccg gcgctgaacg ggttcgtgcc gaagccgaac acgcctttcc
     1021 agtgggagcc gatctgcgaa gagcgcgagc tgaagcggcg gctgaagtgg ctcagcaaga
     1081 atctggcgcg catcccgaac gtcgaggtgc gcgtgatgtc ggcgcgcatc gcgcacgagc
     1141 aggcgctctt ctcttcgggc gaccgctcgg cggcgcgcgt catcgaggcc atgacacgcc
     1201 accgcgacaa cctcgacgcg gccctgcgcg agacgggcgt ggacatcgct ttccacacgc
     1261 ggcgcgcgcg cccttacgcg gaggtgctgc cgtgggagat cgtggacgca ggattggggc
     1321 gcgagttcat ggagaaggaa cacacgcgtg cgcagatcgc gcgctcgacg gcgccgtgcc
     1381 cctcggtcga tacgtgcacg cgctgcggcg tctgcccgac gacgtggctc gccgccgcgc
     1441 ccgcggggct ggtgcagctt aaaggcgtgg ccgccggcgc gtaaggaagg ttaaaaggta
     1501 aaaaggggaa gaggggaaaa gggagaagca gcgacttgct ttcttccctt ttcccctctt
     1561 ccccttttta ccttttcccc tctgtcttta attgaggaag cggaagtcgg tggcgtcgcc
     1621 ggcgagttgt tcgtcggcgg cgtagcaggc ggcgacctcg gtttccgacc aggagatgag
     1681 gagcgcttcg gccttcctgc cgtcgagggg gcgcgagaag aagaagccct ggccgtattc
     1741 gcacttgagg gcgcgcagct gggcgagctg ttcggcggtc tcgatgccct cggcgacgac
     1801 ctccatgccg aggttgctgg cgagcgtgat gatggtgcgg acgatctcgg agttctcgtc
     1861 gccggcgccc atcttgccga tgaagaagcg gtcgatcttg agcgtgttga ccgggaagcg
     1921 gtgcaggtac gagagcgaag agtagccggt gccgaagtcg tcgatgccga cgtggacgcc
     1981 gagcgcgcgc agttgcttga gcatggagct ggcggtctcg gcgctcttca tgacgaccga
     2041 ctcggtgatc tccagcttca gacagcgcgg gtcgaggtcg gtctcttgca gcgtgcgctt
     2101 gatgcggtcg atgaggtcgg gctgcgcgaa ctgcttgctc gacaggttga cgctgacggt
     2161 gagcgggcgg aaaaccgggg atagcttctg ccactcgcgc atctggcggc acgactcttc
     2221 gagcacccac tggccgatct cgacgatgag gccggtgtcc tcggcgacag ggatgaactc
     2281 ggcgggcgag acgaggccgc gctcggggtg ctgccagcgc gcgagcgcct cgaaaccggc
     2341 gatgcgctcg gtctcgatgt tgacgatggg ctggtagtag acgcgcagct cgccgcgctc
     2401 gaccgcgcgc ctgaggtcgc tttcgagttg cagcagggcg acggcgcgcg cgtgcatcac
     2461 cgtgtcgaag acctcgtggc gggccttgcc gttctccttg gcgcggtaca tcgcggtgtc
     2521 ggcgtcgcgc aggatgttct cggggtgctc gtagccgatt gtgctgagcg tgatgccgat
     2581 gctcgcggtg gtgtacacgt cgtggccgtt gaggttgaag gggcgcgtca gctccttctg
     2641 cacgcgctcg gcgacgcgca ccgcgtcgtg gaagtcgccg aggtcgctca agaggatggc
     2701 gaactcgtcg ccgccgaggc gggccaccat gtcgccgggc cgcacgcagc gttcgagccg
     2761 gcgcgcgatc atcacgagga agcggtcgcc cgcgacgtgg ccgagcgagt cgttgatgtt
     2821 cttgaagcga tccatgtcga ggaagagcac ggcgtagcgg tactcgtcct gctcgacggt
     2881 gcggcggatg gcctcgcgca gttggtcggt gagcagcgag cggttcggca ggcccgtcag
     2941 cgcgtcgtgg aaggcggcgc ggcggaagtg ctcgcggctc tcttcgagct cgcggctgat
     3001 gcggtgctgc tcgctgatgt ggcggttcag ctcctcgacg tggcgctcgg cctgctcggc
     3061 cttggcggcg gcggcggcca tctcgatgtt cttcgtgtag gtgcggtacg tccagtagat
     3121 gatggcgacg acgggcgcga ccgcgacgac ggcgtagtag ccgacggcgt tgcccatctt
     3181 gacgatgatg gccgcggcga aggcgcccgc gaagtaggtg atcgaagtcc agaggtagtt
     3241 gtccgtccac gtctgccaga gcggctggtc ggtcttcagc gcctgcgcga cggcgaccgc
     3301 cgaggtgtta gtaacgtact tgacgac
//
LOCUS       DBNGOH010000500         3149 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1540240, whole genome shotgun sequence.
ACCESSION   DBNGOH010000500 DBNGOH010000000
VERSION     DBNGOH010000500.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3149)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3149)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3149
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1540240"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1294)
                     /locus_tag="AC159P_09845"
     CDS             complement(<1..1294)
                     /locus_tag="AC159P_09845"
                     /inference="COORDINATES: protein motif:HMM:NF013594.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HIR3695795.1"
                     /translation="MRVIETKRARRFSAVLMAAALMLTPLAAAAQTRVEMPKNKYDKR
                     QDVEAGQQAAQQVYQQMPILRDNSIDRYVDDVGQRLVSAIPEEFRHPEFRYSFDVVDA
                     RDINAFALPGGPMFINRGMIEAARTEGEMAGVMAHEISHVALRHATAQATETQKFQIG
                     SVLGQIAGAVIGGVPGAAIGTAAQIGFGAGALKYSRKYETQADILGAQIMARAGYDPR
                     DLANMFRTIEQQGGGARGPEWLSSHPNPGNRYQRINQEAALLRVNPESARQDSRQFRD
                     VQTRLRGLPPARTMEEIARSGQRYPNQGGGTTTSYPRQDDNYARGERVAYPSTRYSAQ
                     GTNLFRANIPNNWRALGSDGNGASFAPEGAYGSSGITHGVLFSAERAGYNDLQRASQQ
                     LVQSLTQSNNYLRQSGQWRRTTVNGRAALATTLSGRSPV"
     gene            complement(1569..>3149)
                     /locus_tag="AC159P_09850"
     CDS             complement(1569..>3149)
                     /locus_tag="AC159P_09850"
                     /inference="COORDINATES: protein motif:HMM:NF017745.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 family protein"
                     /protein_id="HIR3695796.1"
                     /translation="NALTAFAEWLERDLLPRSRGDFRVGAEGFRKKLLYEEMVETPLA
                     QLVREGERELRETQDAMRAVAEEVAPGRGVAAALGLLARERPTAEGLVGETRAELDRI
                     RAFVRTEQIMTPPTRENLSVAETPEYARALSFASLDAPGPFERVATESYYYVTPPDPA
                     WDARRREEHLGHYNRYQLPVISMHEAYPGHYYQLLKAKQQGSRVRAAFGAASFVEGWA
                     HYCEQMMLDEGFGGNNPKLRLAQLNAALLRLCRYLVGIRLHTGGMSYEEAVEFFVREG
                     FLARTSAEREARRGTLDPTYLVYTLGKMEILRLREEWRRHMGDSFRLGEFHDRLLSYG
                     MPPVRVLRMAMLGEGGGAPSRHGAGGKNGTAPAGAQGEEAKPIDFSVLATGAMSDYEG
                     ARHLELVTDETEWRRVWARVGGGRPMPDVSFNTRAVVVVYQGRQPTGGYSVEITGIKR
                     VGTVLAVNVVERRPASGDVTTQVVTSPFVAASIPRPPTGATVRFEGARGAAEPQPNRK
                     KTVRPRGTTRRGRRGRGR"
BASE COUNT          429 a         1088 c         1101 g          531 t
ORIGIN      
        1 tgacgggcga gcggcccgag agcgtggtgg ccagcgcggc gcgaccgttg acggtcgtgc
       61 ggcgccactg gcccgactgc cgcaggtagt tgttgctctg cgtcaggctc tggacgagct
      121 gctgcgaggc gcgctgcaag tcgttgtacc cggcgcgctc ggcggagaag aggacgccgt
      181 gcgtgatgcc gctcgacccg taggcgcctt cgggcgcgaa gctcgcgccg ttgccgtcgc
      241 tgcccagagc gcgccagttg ttcgggatgt tggcgcggaa gaggttggtg ccctgcgcgc
      301 tgtagcgcgt cgaagggtag gcgacgcgct cgccgcgcgc gtagttgtcg tcctgccgcg
      361 gatagctggt cgtcgtgccg ccgccctgat tcgggtagcg ctggccggag cgcgcgatct
      421 cttccatcgt ccgcgcgggc gggagaccac gcaagcgcgt ctgcacgtcg cggaactggc
      481 gcgagtcctg ccgcgcgctc tccgggttca cgcgcaggag cgccgcctcc tggttgatgc
      541 gctggtagcg gttgccgggg ttcgggtggc tggagagcca ctcggggccg cgggcgccgc
      601 cgccctgctg ctcgatggtg cggaacatgt tggcgaggtc gcgcgggtcg tagccggcgc
      661 gcgccatgat ctgcgcgccg aggatgtcgg cctgcgtctc gtacttgcgg ctgtacttca
      721 gcgcgcccgc gccgaagccg atctgcgccg ccgtgccgat ggccgcgccg ggcacgccgc
      781 cgatcaccgc gcccgcgatc tgcccgagca cggagccgat ctggaacttc tgcgtctcgg
      841 tggcctgcgc cgtggcgtgc cgcagcgcga cgtgcgagat ttcgtgcgcc atcacgccgg
      901 ccatctcgcc ctctgtgcgc gcggcctcga tcatgccgcg gttgatgaac atcgggccgc
      961 cgggcagcgc gaaggcgttg atgtcgcgcg cgtccaccac gtcgaacgag tagcggaact
     1021 cggggtgccg gaactcctca gggatcgcgg agacgagccg ttggccgaca tcgtccacgt
     1081 accggtcgat gctgttgtcg cgcaggatcg gcatctgctg gtagacctgc tgtgcggcct
     1141 gctgcccggc ctccacgtcc tgccgcttgt cgtacttgtt cttcggcatc tcgacgcgcg
     1201 tctgcgccgc ggcggcgagg ggcgtcagca tgagcgccgc cgccatcagc acggcggaga
     1261 agcggcgcgc acgtttggtt tcgataactc tcattttatt atcctccgag aagcgcgaga
     1321 ctcgcgccgg cagtctacat ctgaaacact aaagggcggc accccttccc gcttgctcaa
     1381 agccagagga ctgccgcccg aaaacttcgc gtgccctctg gccgtctctt cgaagacacg
     1441 gcccccgtta tcgatcacga ggagagagcg tttaaaaaac ttgccgaaac aagctgccgc
     1501 gagacatcgg tcgtcgcagg cgtttcggac tcttttgaga tgaacccgcg ccacgtgcgg
     1561 gttgctgttt aacgccctct tccgcgccgc cccctgcgcg ttgtcccgcg aggtctcact
     1621 gttttcttgc gattcggttg cggttcggcg gcaccccgcg cgccctcgaa ccggacggtc
     1681 gcgccggtcg gcgggcgcgg gatggaggcc gcaacgaaag gcgacgtgac gacctgcgtt
     1741 gtaacgtctc cggacgccgg acgccgctcc acgacattga cagcaagcac agtgccaacg
     1801 cgcttgatgc ccgttatctc cacgctgtag ccgcccgtcg gctgccggcc ctgataaacg
     1861 acgacgacgg cgcgcgtatt gaaactcacg tccggcatgg ggcgcccgcc gccgacgcgc
     1921 gcccacacgc gccgccactc cgtttcgtcc gtcaccaact ccaaatgacg cgcgccctcg
     1981 taatcgctca tcgcgccggt cgccagcacg gagaagtcta tcggcttggc ctcctcgccc
     2041 tgcgcgccgg caggcgccgt cccgtttttg ccgcccgcgc cgtgcctcga tggagcgccg
     2101 ccgccttcgc cgagcatcgc catgcggagg acgcggacgg gcggcatgcc gtagctgagc
     2161 agccggtcgt ggaactcgcc gaggcggaac gagtcgccca tgtggcgccg ccactcctcg
     2221 cgcaggcgaa gaatctccat cttgccgagc gtgtagacga ggtaggtcgg gtcgagcgtg
     2281 ccgcgccgcg cctcgcgctc ggcgctcgtg cgcgcgagga agccttcgcg cacgaagaac
     2341 tcgacggcct cctcgtagct catgccgccc gtgtgcaggc ggatgccgac gaggtaccga
     2401 cagaggcgca ggagcgccgc gttcaactgc gcgagccgga gcttggggtt gttcccgccg
     2461 aagccctcgt cgagcatcat ctgctcgcag tagtgcgccc agccttcgac gaagctcgcc
     2521 gcgccgaagg ccgcgcgcac gcgcgacccc tgctgcttcg ccttcaggag ctggtagtag
     2581 tggccggggt aagcctcgtg catcgagatg acgggcagtt ggtagcggtt gtagtggccg
     2641 aggtgttcct ccctgcggcg cgcgtcccaa gcggggtcgg gcggcgtgac gtagtagtag
     2701 ctttccgtgg cgacgcgttc gaacggcccc ggcgcgtcga gcgaggcgaa ggagagtgcg
     2761 cgcgcgtact cgggcgtctc ggcgaccgag aggttctcgc gcgtgggcgg cgtcatgatc
     2821 tgttcggtgc ggacgaaggc gcggatgcgg tcgagttcgg cgcgcgtctc gccgacgagt
     2881 ccttcggcgg tcgggcgctc gcgcgcgagc agccccagcg ccgccgcgac gccgcgcccc
     2941 ggcgcgacct cttcggcgac ggcgcgcatc gcgtcctgcg tctcgcgcag ctcccgctcg
     3001 ccttcacgca cgagctgcgc gagcggcgtc tcgaccatct cctcgtagag gagcttcttc
     3061 cggaagcctt cggcgcccac gcggaagtcg ccccgcgagc gcggcagcag gtcgcgctcc
     3121 agccactcgg cgaaggccgt gagcgcgtt
//
LOCUS       DBNGOH010000501         4614 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2272932, whole genome shotgun sequence.
ACCESSION   DBNGOH010000501 DBNGOH010000000
VERSION     DBNGOH010000501.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4614)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4614)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4614
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2272932"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(34..912)
                     /locus_tag="AC159P_09855"
     CDS             complement(34..912)
                     /locus_tag="AC159P_09855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695797.1"
                     /translation="MLKGMLPRLSSALLALAACAPLAAVKTPAARRGAASSAAARPAQ
                     KKSVSHGGASVAHDPALADDIQAETLAPDLLEQESDKPDGIYPEHVAFTLVGLRPPPS
                     PLSVNPVLRVCSVAEYKSAYAVSPRYVRDTERTVRALRALLRRQPARLPAAVPTLPFP
                     DASDAFHAHVRYLRFRGGRGVAYLTQSQQDEGLVNNQSMTYEFRGLTDDGRYYITASL
                     PVAAPFLPATRDVTSADGYTLPPSFYGKNAARHRRAYNAYVHRVRLRLDRLAPEKFQP
                     GLQLYDALLTSLAVGK"
     gene            complement(947..1552)
                     /locus_tag="AC159P_09860"
     CDS             complement(947..1552)
                     /locus_tag="AC159P_09860"
                     /inference="COORDINATES: protein motif:HMM:NF020496.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HIR3695798.1"
                     /translation="MATTKAKTKKKTATKAPVTKAKATEAKTARAATKKGATKKAATK
                     ATKRFRVLLERHATSEATGIVIPFDVPKVFGTRARVPVRGTINGHAFRGSIFPVGDGT
                     HYMVVRRELREAAGVRGGQTIAVTLERDDEPRTVTPPADLARALKGNKEARAAWDKLS
                     YTHRREYAEAIEGAKRPETRQRRIEKTLSQLIAGKKELYDR"
     gene            complement(1568..3160)
                     /locus_tag="AC159P_09865"
     CDS             complement(1568..3160)
                     /locus_tag="AC159P_09865"
                     /inference="COORDINATES: protein motif:HMM:NF015851.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR3695799.1"
                     /translation="MAEQIAPGGGGDREQPQQPRWAKVIDQTRCIGCHACTTACKSEN
                     EVPVGVTRTYVKHVDVGTFPQARRAHQVTRCNQCAHAPCVAACPTTAMFKRADGIVDF
                     DKSVCIGCKACMAACPYDAIFINPEDHSAEKCNFCAHRIDIGLEPACVVVCPVEAILV
                     GDMDDPASKVGRVVSREPVNVRRPEKETLPKLFYKGAHQATLDPLAARRPEGGLFMWS
                     EQQEGAGTVVSGNPLFNNTSAAALLSYDVAHTIPWDWRVSLYTWTKSIAAGAYIVMAV
                     LNVLIYFFAIRDAPPALGRVEGALTVWLWVVPVLSGAFLAATGLLLIWDLEHPARFYM
                     IFTRPQWRSWLVKGAFVIAGYTLVLALHFAASLAGSETLQLWLTIAGLPLAAMTAVYT
                     AYLFAQAKARDLWQNPLLPPHLFVQALLAGSAALVPFALWLEPALVPALGWVLFAASL
                     AHLLMVWGEVSLAHPTAHASLAVWEMTRGRYRTYFIAGLVLSLGGLLAPWLGPLAAVP
                     ALAGLLLHEHAYVQAGQSVPLA"
     gene            3412..4254
                     /locus_tag="AC159P_09870"
     CDS             3412..4254
                     /locus_tag="AC159P_09870"
                     /EC_number="3.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008202107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HIR3695800.1"
                     /translation="MKKALLLTTLLWCSLAGANACAQGAAAARKDFVSTREMERINWM
                     EFREVVPTRIRTVLLPTGTLEPHGVANNGADITAPLAIARRIAQRTNALIAPALPYGI
                     TGSMEAYPGAFQITESAYRPFVKQILEGLAKNGFRNIIILNGHGGPQTAVLNAVAAEV
                     SAERKVRTLVVNWWSFASDVTKQVFGEDGGHAGWNETAFVQAVDPALVHPERYKDDLA
                     TAYPAQGTWSAAPFPSSIGLYQPGQGYPKFDQKKADEYFDKVTDKVGALVTDIIRKWD
                     MAGL"
BASE COUNT          722 a         1516 c         1625 g          751 t
ORIGIN      
        1 gctcatcttc tttgtccgac tgctcggcga cggtcacttg ccgactgcga gcgaggttaa
       61 cagcgcgtcg tacagttgca ggcccggctg aaacttctcg ggcgcgagcc tgtccagacg
      121 cagacgcacg cggtggacgt aagcgttgta cgcgcgcctg tgccgggccg cgttcttacc
      181 gtagaagctc ggcggcagcg tgtagccgtc ggcgcttgtg acatcgcggg tggcgggcag
      241 gaagggcgcg gcgacgggca aagaggcggt gatgtagtag cgcccgtcgt cggtcaggcc
      301 gcggaactcg taagtcatgc tctggttgtt gaccagcccc tcgtcctgct gcgactgcgt
      361 gaggtaggct acgccgcgcc cgccgcggaa gcgcagataa cggacgtgcg cgtggaaggc
      421 gtcgctcgcg tcggggaagg gcagcgtggg gactgcggcg ggcagacgcg cgggctgccg
      481 ccgcaagagc gcgcgcagcg cgcgcacagt ccgttcggtg tcgcgcacgt agcgggggct
      541 gaccgcgtag gcgcttttat actcggcgac cgagcagacg cgcaggacgg ggttgaccga
      601 gagcggcgac ggcggcgggc gcaggccgac gagcgtgaag gcgacgtgct ccgggtagat
      661 gccgtcgggc ttgtccgact cctgttcgag caggtcgggg gcgagcgtct cggcctggat
      721 gtcgtcggcc agcgcggggt cgtgcgcgac gcttgccccc ccgtggctca cagacttttt
      781 ctgcgcgggc ctggcggcgg cagaagaggc ggcgccgcgc cgcgccgcgg gagtcttgac
      841 cgccgcgagg ggcgcgcagg cggcgagcgc cagcagcgcg gacgagagtc gggggagcat
      901 acctttcaac atgcttgggg cgtgcctttc atcatcggtc gggaagctac ctgtcgtaca
      961 gctctttctt cccggcgatt agttgagaga gcgttttctc gatgcgtcgc tgcctcgtct
     1021 cgggccgctt cgcgccttcg atggcttcgg cgtactcgcg ccggtgcgtg tagctgagtt
     1081 tgtcccacgc ggcgcgcgct tccttgttcc ccttgagcgc gcgggcgagg tcggcgggcg
     1141 gcgtgacggt gcgcggctcg tcgtcgcgct ccagcgtgac ggcgatggtc tggccgccac
     1201 ggacgcccgc ggcctcgcgc agctccctgc ggacgaccat gtagtgcgtg ccgtcgccca
     1261 cggggaagat ggagccgcgg aaggcgtggc cattgatcgt gccgcgcacg gggacgcgtg
     1321 cgcgcgtgcc gaagaccttc ggcacgtcga aagggataac gatgccggtc gcctcgctcg
     1381 tcgcgtgcct ttcgagcagc acgcggaaac gcttcgtcgc cttcgtcgcg gccttcttcg
     1441 tcgccccctt cttcgtcgcc gccctcgccg tcttcgcttc ggtcgccttc gccttcgtca
     1501 caggcgcttt cgtcgccgtc ttcttcttcg tcttcgcttt cgtcgtcgcc atcgttcacg
     1561 tctcctttca ggcgagcggc acggactggc cggcctgcac gtaggcgtgt tcgtggagca
     1621 gcagcccggc gagcgcgggc acggcggcca aggggccgag ccacggcgcg agcaggccgc
     1681 cgagcgagag cacgaggccc gcgatgaagt atgtgcggta gcgcccgcgc gtcatctccc
     1741 aaacggcgag gctcgcgtgc gcggtcgggt gcgcgagcga gacctcgccc cagaccatca
     1801 ggaggtgcgc gaggcttgcc gcgaagagaa cccacccgag cgccgggaca agcgccggtt
     1861 cgagccacag cgcgaagggc acgagcgccg ccgacccggc gagcagcgct tgcacgaaca
     1921 ggtgcggcgg cagcagcggg ttctgccaga ggtcgcgcgc cttggcctgc gcgaaaaggt
     1981 aggcggtgta gacggcggtc atcgccgcga gcggcaggcc cgctatcgtg agccagagtt
     2041 gaagcgtctc gctcccggcg aggctcgcgg cgaagtggag cgcgagcacc agcgtgtaac
     2101 cggcgatgac gaacgcgccc ttgacgagcc agctccgcca ctgcgggcgc gtgaagatca
     2161 tgtagaagcg cgcggggtgc tccaagtccc agatgagcag caggccggtc gcggcgagga
     2221 acgcgccgga caggacgggc acgacccaga gccagaccgt gagcgcgccc tcgacgcgcc
     2281 ccagcgcggg cggcgcgtcg cgtatggcga agaagtagat gaggacgttg agcaccgcca
     2341 tcacgatgta ggcgcccgcc gcgatgctct tcgtccaagt gtagaggctc acgcgccagt
     2401 cccaagggat cgtgtgtgcc acgtcgtaag agaggagcgc cgccgccgac gtgttgttga
     2461 agagtggatt gccggagacg accgtgcccg cgccctcttg ctgctcgctc cacatgaaga
     2521 ggccgccctc gggacggcgc gcggcgagcg ggtcgagcgt cgcctgatgc gcgcccttgt
     2581 agaagagctt gggcagcgtc tccttctcgg gccgccgcac gttgacgggt tcgcggctga
     2641 cgacgcgccc gaccttcgac gcggggtcgt ccatgtcgcc gaccaaaatc gcctcgaccg
     2701 ggcagacgac gacgcacgcg ggttcgagtc cgatgtcgat gcggtgcgcg cagaagttgc
     2761 acttctccgc cgagtggtct tcggggttga tgaagatggc gtcgtagggg caggccgcca
     2821 tgcacgcctt gcagccgatg cagaccgact tgtcgaaatc gacgatgccg tccgcgcgct
     2881 tgaacatcgc ggtggtcggg cacgcggcga cgcacggcgc gtgcgcgcac tggttgcagc
     2941 gcgtcacctg atgcgcgcgc cgcgcctgcg ggaacgtgcc gacatcgacg tgcttgacgt
     3001 aggtgcgcgt gacgccgacg gggacttcgt tctccgactt gcacgcggtc gtgcaggcgt
     3061 ggcagccgat gcagcgcgtc tggtcgatca ccttcgccca gcggggctgc tgtggttgct
     3121 ctcggtcgcc gccgccgccg ggcgcgatct gttccgccat caagactcct ttcgcgccgt
     3181 cgcccgcgcg tgcgcgcagg gcgagccggt gcggcgagga aagccgttca atgtttccgc
     3241 cggatgaagt atcctctgtc gaactgcgtt gaagaacgct tctacacttt aacggcgggc
     3301 gagtcaatcg taatcgtccc cgcgcgcccg tgggacgccg ggcgctaacg gcccggcccg
     3361 ctgcgggcgg ggaaggcgaa agaccgatca cttcgaggcg gaggatgtga gatgaaaaaa
     3421 gcgctgctct tgacgactct cctctggtgt tcactcgcgg gcgcgaacgc ctgtgcgcag
     3481 ggcgcggctg ccgcgcggaa ggacttcgtc tcgacgcgcg agatggagcg catcaactgg
     3541 atggagttca gagaggtcgt gcccacgcgc atccggaccg tgctcctgcc gaccggaacc
     3601 ttagagccgc acggcgtcgc caacaacggc gccgacatca cggccccgct cgccatcgcg
     3661 cgccgcatcg cgcagcgcac gaacgccctc atcgcgcccg cgctccctta cggcatcacc
     3721 ggcagcatgg aggcttaccc cggcgcgttc cagatcacag agtccgctta ccgccccttc
     3781 gtcaaacaga ttctcgaagg gctggcgaag aacggcttcc ggaacatcat tatcctcaac
     3841 ggccacggcg ggccgcagac ggcggtgctc aacgccgtcg cggcggaggt ctcggcggag
     3901 cgcaaagtgc gcacgctcgt cgtcaactgg tggtcgttcg cgtccgacgt gaccaagcag
     3961 gtcttcggcg aagacggcgg ccacgccggc tggaacgaga ccgccttcgt gcaggccgta
     4021 gaccccgcgc tcgtccaccc cgaacgctac aaagacgacc tcgccaccgc ctaccccgcg
     4081 cagggcacat ggtctgccgc gcccttcccc tcctccatcg gcctctacca gcccggacag
     4141 ggctacccga agttcgacca gaagaaggcc gacgagtatt tcgacaaagt caccgacaag
     4201 gtcggcgccc tcgtcacgga tatcatccgc aagtgggaca tggccgggct gtgagcggga
     4261 agggcgaaaa ggcgaaaagg gcaaaaggcg aaagggcaag ggggaaggga aggggcctgc
     4321 gcggggcgcg tcagtcgtct tctttcgtct cttttcggca cgacggcgtg atgactaaat
     4381 cgcgcggcgg gcgtatactg tctccgccgt tcccctgaac atctctcccg tcccccgcgt
     4441 ttctctcccc gctccccctt tgttgattcc ccgagcattt cgaatcaaaa ggaggaagcg
     4501 cgcgggtcgc gccgtccgct gctttgccgg tcaggcgcgt cgcgcgggag aaaaagaatg
     4561 ttgcacggaa caagacgcgt cagcgtcgcc tttgtcgtcg tcgtcctcgc gttg
//
LOCUS       DBNGOH010000502         2414 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3924280, whole genome shotgun sequence.
ACCESSION   DBNGOH010000502 DBNGOH010000000
VERSION     DBNGOH010000502.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2414)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2414)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2414
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3924280"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(188..784)
                     /gene="kdpC"
                     /locus_tag="AC159P_09875"
     CDS             complement(188..784)
                     /gene="kdpC"
                     /locus_tag="AC159P_09875"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013580788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpC"
                     /protein_id="HIR3695801.1"
                     /translation="MKSTRVTKNLVTASLMTVVTVVILGLVYPLAVTGLARLVFADKA
                     DGQLLRRGDGTVVGSSLIGQPFSSPGYFRPRPSAAGSVGYDAGASGGSNLGPTNRKLI
                     ERVKADVEKLHAENPGAAVPVDLVTTSASGLDPHVSPAAAELQVPRVARERGMREEDL
                     RRLVAAHSEGRQFGILGEPRVNVLLLNLALDGIKSDED"
     gene            complement(897..>2414)
                     /gene="kdpB"
                     /locus_tag="AC159P_09880"
     CDS             complement(897..>2414)
                     /gene="kdpB"
                     /locus_tag="AC159P_09880"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020715566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HIR3695802.1"
                     /translation="TRVLSDQIRIKITSNPGETFLDRMIALVEGAERQKTPNEIALNI
                     LLAGLTIIFLLAVVTLQPFAVYSNAPQSAFVLVSLLVCLIPTTIGGLLSAIGVAGMDR
                     LIQHNVLAMSGRAVEAAGDVNTLLLDKTGTITLGNRQAAEFIPLAGVSEAELADAAQL
                     SSLADETPEGRSVVVLAKELYNLRGRELAPHEAHFIPFTAQTRMSGVDFDGRQIRKGA
                     VDAVERYVASSNGGALSPRELRETVERVARAGGTPLVVAENARPLGVIYLKDIVKGGM
                     RERFEQLRRMGIKTVMITGDNPLTAETIAREAGVDDFLAEATPEDKMTLIRREQAEGK
                     LVAMTGDGTNDAPALAQADVGVAMNTGTQAAKEAGNMVDLDSNPTKLIEIVEIGKQLL
                     MTRGALTTFSISNDVAKYFAIIPAMFAGAFPVLDALNVMGLRTPHSAVLSAVIFNALV
                     IVALIPLALRGVKYRPLSAAKLLRRNLLVYGLGGLVVPFVGIKIIDVIIHAVGLA"
BASE COUNT          417 a          780 c          797 g          420 t
ORIGIN      
        1 atccggtagg tcttgccgac accggcagcc gcaccgatgt agacacgtag gctcgcccgt
       61 tcgccctctt tcaacttggc gagcaacgct tccggcgatg ggcgggcggc gacagccata
      121 tacgaaaata agtgatgagg acagaggact gaggactgag tgaagacaaa atactgttac
      181 tcagtcctca gtcctcatca ctttttattc cgtcgagcgc gaggttgagt agcagcacgt
      241 tcaccctcgg ctcgccgagg atgccgaact gtcgtccttc gctgtgggcc gcgacgagcc
      301 gtctcaagtc ttcctcgcgc ataccgcgct cgcgtgcgac gcgcgggact tgaagctctg
      361 cggcggcggg cgagacgtgc gggtcgaggc cggaggccga agtcgtcacc aggtctacag
      421 ggacggcggc gccgggattc tcggcgtgca gcttttccac gtcggccttc acgcgctcga
      481 ttagcttgcg gttcgtgggg ccgaggttcg agccgcccga agcgcccgcg tcgtaaccga
      541 ccgacccggc ggcagagggc cgcgggcgga agtagccggg cgacgaaaaa ggctggccga
      601 tgagcgacga gccgacgacc gtaccgtcgc cgcggcgaag caactgcccg tctgccttgt
      661 cggcaaagac gagccgtgcc agccccgtca ccgcgagcgg atagacgagg ccgaggatga
      721 cgacggtgac gacggtcatc agcgaggccg tgaccagatt ctttgtgact ctcgtactct
      781 tcatgtttat gtcgtggccg tgtccgccgg atgttttgcg ggggagcgcc gcgcgccggc
      841 ctgttcaccc ttggggcgag gcaaaccggg ctgatgcgcc tcccccgcag accctctcac
      901 gccagaccca cggcgtgaat gattacgtca atgatcttga tgccgacgaa cgggacgacc
      961 agcccaccca ggccgtagac cagaaggttg cgccgcaaga gctttgccgc cgacagcggg
     1021 cgatacttga cgccgcgcag cgcgagcggg atgagcgcga cgatgacaag cgcattgaag
     1081 atgaccgccg acagcaccgc cgaatgcggc gtccgcaggc ccatcacatt gagcgcgtcg
     1141 agcaccggga acgcgcccgc gaacatcgcg gggatgatgg cgaaatactt cgccacgtcg
     1201 ttagagatgg agaaggtggt caaagcgccg cgcgtcatca ggagttgttt gccgatctcg
     1261 acgatctcga tgagcttggt cgggtttgag tcgaggtcaa ccatgttgcc ggcctctttc
     1321 gcggcctgcg tgccggtgtt catcgccacg cccacgtcgg cctgcgcgag cgcgggcgcg
     1381 tcgttcgtcc cgtcgccggt catggcgacg agtttaccct cggcctgctc gcggcggatg
     1441 agggtcatct tgtcttcggg cgtcgcttcg gcgaggaagt cgtccacgcc tgcctcgcgc
     1501 gcgatggtct ccgcggtgag cgggttgtcg cccgttatca tcacggtctt gatgcccatc
     1561 cggcgcaact gctcgaagcg ttcgcgcatc ccgcccttca cgatgtcctt caggtagatg
     1621 acgccgagcg ggcgcgcgtt ctcggccacg acgaggggcg tgcctcccgc gcgcgcgacg
     1681 cgctcgaccg tctcgcgcag ctcgcgtggt gacaacgcgc cgccgttcga cgacgcgacg
     1741 taacgctcga cggcgtccac cgcgcccttg cgaatctggc ggccgtcgaa gtccacgccg
     1801 gacatgcgcg tctgcgccgt gaaggggatg aagtgcgcct cgtgcggcgc gagttcgcgg
     1861 ccacgcaggt tgtaaagctc ttttgccagc acgacgacgg agcggccttc cggcgtctcg
     1921 tcggcgagcg aagagagctg cgccgcgtcg gccagctccg cctcgctgac gcccgcgagc
     1981 gggatgaact ccgcggcctg acggttcccg agcgtgatgg tgccggtctt gtccagcagc
     2041 agcgtgttca catcacccgc cgcttcgacc gcgcgccccg acatcgccag cacgttatgt
     2101 tgaatcagtc tgtccatccc tgcgacaccg atggccgaga gcagtccgcc gatggtcgtc
     2161 gggatgagac agacgagcag gctgacgagc acgaaggccg actgcggcgc gttcgagtag
     2221 acggcgaacg gctggagcgt cacgaccgcg agcaggaaga tgatcgtgag gcccgcgagc
     2281 aaaatattta acgcgatctc gttcggcgtc ttctgccgct ccgcgccttc caccagcgcg
     2341 atcatccggt cgagaaaggt ctcccccgga ttcgacgtga tcttgattcg aatctgatcc
     2401 gacaacacgc gagt
//
LOCUS       DBNGOH010000503         4466 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3006882, whole genome shotgun sequence.
ACCESSION   DBNGOH010000503 DBNGOH010000000
VERSION     DBNGOH010000503.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4466)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4466)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4466
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3006882"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..380
                     /locus_tag="AC159P_09885"
     CDS             <1..380
                     /locus_tag="AC159P_09885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TolB family protein"
                     /protein_id="HIR3695803.1"
                     /translation="ALQPAVSPDGKLIACYYLDEQTRAMKIALLPFEGGDPVKLFDLP
                     QTSNRGQPPRWTPDGRALTYINTRGIVSNIWLQPVDDGQHKLLTDFKTDRIFSFDWSR
                     DGKRLAVSRGVVDSDVVLIGNFK"
     gene            586..792
                     /locus_tag="AC159P_09890"
                     /pseudo
     CDS             586..792
                     /locus_tag="AC159P_09890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_267195200.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            834..1286
                     /locus_tag="AC159P_09895"
     CDS             834..1286
                     /locus_tag="AC159P_09895"
                     /inference="COORDINATES: protein motif:HMM:NF025910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPR end-of-group domain-containing protein"
                     /protein_id="HIR3695804.1"
                     /translation="MKNARRYDEAIAHLKNALELDENFVPTYGQLAQAHWLKGNYAES
                     VEALARSREIKGEHEVASRMRESFAKGGWEGYLRYMTEDPRVPHISYFVAPYHAALGE
                     RDKAFAALNKSYENREAALQSLKVDPRLDPLRPDPRFSELLRKVGLPQ"
     gene            complement(1655..3121)
                     /locus_tag="AC159P_09900"
     CDS             complement(1655..3121)
                     /locus_tag="AC159P_09900"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014081.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR3695805.1"
                     /translation="MLALVLSAAPTRADDLAFSHVTVVDVVEGRLVRDRVVRVAGGRI
                     VEVAPAAGARIPKGTRVVDARGKFLIPGLWDMHTHLLGGVPPGCPEVTFPLMVAHGIT
                     GVREVGSYLDFLYAWREEVESGRSVGPRIVGTDRLIDGVPPVYPGIASVATTPGNARQ
                     TVDILSRRGADFVKAYEMLSRDAFFALVEQAKKSGLPVVAHVPLSVLAGEASDAGVRS
                     FEHLRNIEFACSREADTLLAERIALLEAGVGRVGSELRSEIHSAQRTRAYDTYDPGRC
                     TALLRKFAANGTWQTPTLFLNTREVRRPDLLEDVRERLRWVPDRQRAEWEAWTKGVTS
                     MKPEAAAALRRRADWEVQLVRRMNEEGVGLLAGTDVATPWTVPGAALHDELSALVEAG
                     LTPLESLRTATLNPARYFEKTGEFGAVAPGMAADLVLIDGDPLADIRNTRRISGVVAN
                     GRYLDRPALDRMLAEAERLARDGGHDPIHRPGRGRPSK"
     gene            complement(3308..4291)
                     /locus_tag="AC159P_09905"
     CDS             complement(3308..4291)
                     /locus_tag="AC159P_09905"
                     /inference="COORDINATES: protein motif:HMM:NF016292.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR3695806.1"
                     /translation="MACVYSRRWFVTRLGLFGCALPLTGYAQLAESRRVRRIGFLIGP
                     APTLIAAFEEELRRLGYVDGKDIVIERRVHTSDLAVQAAELARMDLDLIVAAALPQAL
                     EVRKNNPAMPMVIGTCPGMVSNGFAKSLKRPGGHYTGMDELPPGVTAKRLHLLKTAAP
                     TVSRVALLSTTPGRGGHEAQLADAEKAAKSLRLTVRPYRAASLKELEAALAAIVSDRM
                     DGLANFQGGLSLTHRQLIVDFAAKHRLPAVYQSAFFVEAGGLMAWAPDQEEQFREAAR
                     YVDKILRGVKPGHLPIHFPRRYYLTINKSAAGDLGLALPAALVTQADRVLP"
BASE COUNT          768 a         1493 c         1471 g          734 t
ORIGIN      
        1 atgcgctaca accggccgtc tcccctgacg ggaagctgat cgcctgctac tacctcgacg
       61 aacagacgag agccatgaag atcgcgctcc tgccgttcga gggtggggac ccggtcaaac
      121 tatttgatct cccgcagact tcgaatcgag ggcagcctcc tcgctggacg cccgacggtc
      181 gcgccctgac ctatatcaac acgcgcggca tcgtctccaa catctggctt cagcccgttg
      241 acgacggaca acacaagctg ttgaccgact tcaagaccga ccggatcttc tctttcgact
      301 ggtcacgcga cggcaagcgg ctggcggtct cgcgcggcgt cgtggatagt gatgtagtct
      361 taatcggcaa tttcaaatga tcgcggttgg cacgcgacta gggcgctacg aaatccgctc
      421 gaagataggc gcgggcggga tgggtgaggt ctatctggcg gaagatacgg agctcgaacg
      481 ccttgtcgcg ttgaaaattt tgccctcgga gctggcgacc gatcatgaac ggatgtcttt
      541 cgaaggtact acgactatga aagggtcgag cggctgacag gcgcggcctt ctacttcgcg
      601 cggccctacc actcgtggga gcgcggcagc aacgagaacg ccaacgggct gctgcggtag
      661 tacctgccga agggacagag catggccggg ctgagccagc accggtgcaa cgccatcgcc
      721 cgtaaactga acacgcggcc ccgaaagcgt ctcgacttca ggacgccgct cgaatgcttc
      781 aacgagtcct agtcagtgtt gcacttcaat gttgatgtca ggacgccggc ctgttgaaga
      841 acgcgcggag gtacgacgag gcgatagcgc acctgaagaa tgcgctcgaa ctggacgaga
      901 acttcgtccc cacgtacggc cagttagcgc aggcacactg gcttaaaggc aactacgcgg
      961 agagcgttga agcgctggcg cggtcgcgtg aaatcaaagg cgagcatgaa gtcgcctccc
     1021 ggatgaggga gagctttgcg aagggcgggt gggaagggta cctgcggtac atgaccgaag
     1081 acccgcgggt gccccatatc tcttattttg tcgcgcccta tcacgcggca ctgggcgaac
     1141 gggacaaggc gttcgccgcc ttaaacaagt cttacgagaa ccgcgaggcg gctttgcagt
     1201 cgctcaaggt tgacccgcgc ctcgacccgc tgcgcccgga cccgcggttc tcggaactgc
     1261 ttcgcaaagt agggctcccg cagtaatcat gaagcgcccc ggcgcgacgg ccgcgtcgca
     1321 ggaaatgctg aggggcgact gaggacgtga cagaccttac ctatgtggct acgccgacgg
     1381 ggctcccgac gaactttgcc tgccccgccg agtctgttcc gcctggccta tagatcggcc
     1441 ccccgcgtca tgcataacgc ctcgacccat tgagcaaggg tccgcaaggg aaatgtactc
     1501 gtgcccttac atcctcagag gcaggtgcgg atgatgtcga agcacagggt tctgaagagt
     1561 gggatgcgct acgaccgcgg gcgggagaat tcgccgttga atggccccgg tcataggccc
     1621 aaagctccgg ggtaagcccg gaactcactc cgagtcactt cgacggccgc ccccgccccg
     1681 gccgatggat ggggtcgtga cccccgtccc gcgccagccg ctcggcttcc gccagcatcc
     1741 ggtcgagcgc cgggcggtcc aggtagcggc cgttcgccac cacgccggag atgcgccgcg
     1801 tgttgcggat gtcggccagc ggatcgccgt cgatcaggac gagatccgcg gccatccccg
     1861 gcgcgacggc tccgaactcg ccagtttttt cgaagtagcg agccgggttg agggtggctg
     1921 tgcgcagcga ttcgagcggc gtcagccccg cctcgacgag tgcggacaac tcatcgtgga
     1981 gcgcggcgcc ggggaccgtc cacggcgtcg cgacgtcggt gcccgccagc agcccgacgc
     2041 cctcttcgtt catgcgccgc acgagctgca cctcccagtc ggcgcgccgc ctgagcgccg
     2101 cggccgcctc cggcttcatg gaggtgaccc ccttcgtcca tgcctcccac tccgctcgct
     2161 ggcggtctgg cacccaacgc agtctctccc gcacgtcttc aagcaggtcg ggacggcgca
     2221 cctcccgcgt attgaggaac agtgtcggcg tctgccaggt tccgttggcg gcgaactttc
     2281 ggagcagcgc cgtgcagcgc cccgggtcgt aggtgtcgta ggcgcgcgtc cgctgcgcgg
     2341 aatggatttc cgagcgcagc tcggaaccga cccgcccgac ccccgcttcg agcaaggcga
     2401 tccgctcggc aagcagcgtg tcggcctcgc gcgagcaggc gaactcgata ttgcgcaggt
     2461 gctcgaagct cctgaccccg gcgtccgacg cctcgcctgc gaggaccgag agcggcacgt
     2521 gcgcgaccac gggaagcccg ctcttcttcg cctgctcgac gagggcgaag aacgcgtcgc
     2581 ggctgagcat ctcgtaggcc ttgacgaagt cggcgccgcg ccgcgacagg atgtccacag
     2641 tctgtctggc gttgcccggc gtcgttgcga cgctcgcgat ccccgggtag acgggaggca
     2701 ccccgtcgat cagccggtcg gtcccgacga tccgcggccc gacgctgcgg cccgactcga
     2761 cctcttcgcg ccacgcatac aggaagtcca ggtacgatcc gacctcgcgg acgccggtga
     2821 tgccgtgggc gaccatcagc gggaaggtca cctcggggca acccggcggc accccgccca
     2881 aaaggtgggt gtgcatgtcc cacaggcccg ggatcaggaa cttgcccctc gcgtcgacga
     2941 cacgggtgcc cttcgggatc cgggcgccgg ccgcgggcgc cacctcgacg atgcgcccgc
     3001 cggcgacccg caccacccga tcacggacga gccttccttc gacgacatcg acgaccgtca
     3061 cgtgactgaa ggcgaggtcg tccgcgcggg tgggcgctgc cgagagcacc aaagccagca
     3121 tgagtgcagc ggcgagattt cttatcctac atactttcat gagttgcgtg tggccctaat
     3181 gccgggcatc ggcggcgcgc acgggccttt gccgataaaa gacttgctca ttgcgcgccc
     3241 gccggatgcc gttgttaggc cgctgcacat gggcgcgagc ccgcatcctt agggcaccgc
     3301 cagtctctta cggcaacacc cggtcggcct gcgtgacgag ggccgcaggc agggcgagtc
     3361 ccaggtcccc ggcggcgctc ttgttgatcg tcaggtagta ccgacgggga aagtggatcg
     3421 gcaggtggcc cggcttcacg cccctgagga ttttatcgac gtatcgcgcc gcctcgcgga
     3481 actgctcctc ctggtccggc gcccacgcca tcagcccccc ggcctcgacg aagaaggccg
     3541 actggtagac cgccggcagg cggtgcttcg ccgcgaagtc cacgatgagc tggcggtggg
     3601 tgagcgacag cccgccctgg aaattcgcga ggccgtccat ccggtcgctg acgatggcgg
     3661 cgagcgccgc ttccagttct ttcagcgaag cggcccggta gggcctcacc gttaggcgga
     3721 gcgacttggc ggccttctcg gcgtcggcaa gctgagcctc atgcccaccc cgccccggcg
     3781 tcgtcgagag gagcgcgacg cgcgacaccg tgggtgcggc ggtcttcagg agatggagcc
     3841 gcttcgccgt cacgcccgga gggagctcgt ccatcccggt gtagtgaccg ccggggcgct
     3901 taaggctctt ggcgaatccg ttgctgacca tgcccggaca ggtgccgatg accatcggca
     3961 tggcggggtt gttcttgcgc acctcgagcg cctgcggcag cgccgcggcc acgatcaggt
     4021 cgaggtccat gcgggcgagt tcggccgctt gcacggccaa gtccgacgtg tggacgcgtc
     4081 tttcgatgac gatgtccttc ccgtccacat accccagccg gcgcaactcc tcttcgaacg
     4141 ccgcgatcag cgtcggcgcc gggccgatta gaaagccgat gcgccggacg cggcgcgact
     4201 cggcgagctg agcatacccc gtcagcggga gcgcgcagcc gaatagcccg aggcgggtga
     4261 cgaaccaacg gcggctgtag acgcatgcca tgcttcaaac tccactccgg cggcctcagg
     4321 cccggcgccg gcggccgcgg cgagctgtga ttatacgcga gctgtcgatg aaggcatcgc
     4381 cgttcgcggt cggatgcacg ccgcggttac gcgcgcttcc gcaaggcacc tcatcgttgt
     4441 tggacctggg tggcgtgcgc ccagac
//
LOCUS       DBNGOH010000504         1993 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1175581, whole genome shotgun sequence.
ACCESSION   DBNGOH010000504 DBNGOH010000000
VERSION     DBNGOH010000504.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1993)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1993)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1993
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1175581"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            359..>1993
                     /locus_tag="AC159P_09910"
     CDS             359..>1993
                     /locus_tag="AC159P_09910"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HIR3695807.1"
                     /translation="MRRPLIALPLALVLALAPPTAPAALAAPTAAPQKAVDQAQLPAR
                     YAVRLRQFETEVRRQMELDRTTGLTIGFVKDGAAWAKGFGYADLENKTPMKAESAYRL
                     ASVTKPMTAIAVLQLAEQGKLDLDAEVQTYVPYFPKKKWPVTVRQLLGHLGGIPHYVN
                     REAELRIKTRKTTRESIAIFENYDLVAEPGTRYSYSSYGYNLLGAIVESASGQSYGDY
                     MRDKVWRPLGMGDTRMDDPLELIPNRVRGYQLVDGKLANSEFVDISSRFAAGGTRSTV
                     PDLLKFAKGVMQGKLLSKASYDLMYDSMHTREGRFTNYSAGWETSHPNGRFMVAHSGG
                     QNETRTLLWVFPGKNFAIAGAVNFEGSSPYWYMQRLFQLLTDEPFEVSAYTGDRLKDA
                     LYVGLRDTFGEGLAHFARHRQPLTKTDAEAAEAFAYFNKHVSEEALRAAQQETWRRIR
                     EGRHPAGGQSFVKVGSVMAAKLREKYGDARLDTYTAGGPLAFFADYVALYKSQPDFPA
                     ALRFAAPLEQSVARWRASWDKANTAYVRGLSVTPDAG"
BASE COUNT          354 a          723 c          627 g          289 t
ORIGIN      
        1 aattgaccgc cgggacgacg gcacgtatca ggtcgtcaag cactaccggc gcggcgcgcg
       61 cgcgcagtct cataccctcg gcaatctttc cgcggtcgtc gccgaggttc cgggctagag
      121 acgccgcacg tattcaccgc ccgcccaccg actttggcgc aacaaccagg tctcgcgcgt
      181 cgtcttacac agcgtccggc gttcgtgtta acggttaacg gcgacgaagc gtccaccata
      241 agggacgcgt ccgcagcagt cgtcagccgg gacacgcgca ccgccccgtt cccttcttcc
      301 gcgcacaaac gaatatcagt cccgcacggc gggacgcgac acgctaagga gacttacgat
      361 gagaagaccg ttgatcgccc tcccgctcgc gctcgtgctg gccctcgcgc cgccgacggc
      421 gcccgccgcg ctcgcggccc cgactgccgc gccgcagaag gccgtcgatc aggcacaact
      481 gcccgcgcgc tacgccgtca ggctccgcca attcgagacg gaagtccgcc ggcagatgga
      541 actcgaccgc acgacggggc tgaccatcgg cttcgtcaag gacggcgccg cgtgggcgaa
      601 gggcttcggc tacgccgacc tcgaaaacaa gacgccgatg aaggccgagt cggcctaccg
      661 gctcgcgtcg gtgacgaagc cgatgacggc catcgccgtc ctccaactcg cagagcaggg
      721 caagctcgac ctcgacgccg aggtgcagac ctacgtcccc tacttcccga agaagaagtg
      781 gcccgtcacc gtgcgccagc tcctcggcca cctcggcggc atcccgcact acgtcaaccg
      841 cgaggccgag ctgcgcatca agacccgcaa gacgacgcgc gagtccatcg ccatcttcga
      901 gaactacgac ctcgtcgccg agccgggcac gcgctacagc tattcgagct acggttacaa
      961 cctgctcggc gccatcgtcg aatccgcgtc cggccagtct tacggcgact acatgcgtga
     1021 caaagtatgg cgcccgctcg gcatgggcga cacgcgcatg gacgatccgc ttgagcttat
     1081 cccgaaccgc gtgcgcggct atcaactggt ggacggcaag ctcgccaact ccgagttcgt
     1141 ggacatcagc agccgcttcg ccgcgggcgg cacgcgctcg accgtgcccg acctcttgaa
     1201 gttcgccaag ggcgtcatgc aggggaaact gctgtcgaag gcgagctacg acctgatgta
     1261 cgactcgatg cacacgcgcg aggggcgctt caccaactac agcgcgggct gggagacctc
     1321 gcaccccaac gggcgcttca tggtcgcgca cagcggcggg cagaacgaga cgcggacgct
     1381 gctctgggtc tttccgggta agaacttcgc catcgcgggc gcggtcaact tcgaaggctc
     1441 ttcgccctac tggtacatgc agcgcctctt ccagctcctc accgacgaac ccttcgaggt
     1501 gtctgcctac acgggcgacc gcctgaagga cgcgctctac gtgggcctgc gcgacacgtt
     1561 cggcgagggc ctggcgcact tcgcgcgcca ccgccagccg ctcacgaaga cggacgccga
     1621 agcggcggag gcgttcgcct acttcaacaa gcacgtgagc gaggaggcgc tgcgcgccgc
     1681 gcagcaggag acgtggcgca ggatcaggga gggccgccac cccgcgggcg gccagtcgtt
     1741 cgtcaaggtc ggctcggtca tggccgcgaa gctgcgcgag aagtacggcg acgcgcggct
     1801 cgacacctac acggcgggcg gcccgctcgc cttcttcgcc gactacgtcg cgctctacaa
     1861 gtcgcagccc gacttccccg ccgcgctccg cttcgccgcg ccgcttgagc agagcgtcgc
     1921 gcgctggcgc gcgtcgtggg acaaggcgaa cacggcttac gtccgcggcc tctcggtcac
     1981 gcccgacgcg ggc
//
LOCUS       DBNGOH010000505         4098 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3556795, whole genome shotgun sequence.
ACCESSION   DBNGOH010000505 DBNGOH010000000
VERSION     DBNGOH010000505.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4098)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4098)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4098
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3556795"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..426)
                     /locus_tag="AC159P_09915"
     CDS             complement(<1..426)
                     /locus_tag="AC159P_09915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695808.1"
                     /translation="MMKRVLFVVVGLLLASLLSAVALGQEPRAGAPHQGDEKAVRLAR
                     VLEKLGESVGRYQAGLFSIAFTETVREERLKRDLKTPDGKAKEYVFESIALRQPVRDG
                     EEDLYAKNVRRLRAEDGKPAQQGKPYVQKRHKCGGGVGPA"
     gene            complement(471..1346)
                     /locus_tag="AC159P_09920"
     CDS             complement(471..1346)
                     /locus_tag="AC159P_09920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695809.1"
                     /translation="MFKDAFTAIGGATRELLRDWGALAVLAALYGALLFAVYWFFATG
                     VATAGQLALSALLAGFAPLLFFLLQAATANHAAGAERAREVLLRALSDFWKILLVSLP
                     LIALAVLVYYLLNKLQARFPVTAEDAARFVPPGAGPRAAPPPLPLRWQDVTFSALRLL
                     LLGVVLPLAGIHLWLAVARDGLVAALRNAHRWLARAYAPRAVLVYAVGLFLFGFMPYF
                     VVYTTTPVKNGWGELILFGLRLALAFLLTLCGWAVTLGALAKLAPGGEVSTAGEAPPV
                     ADPPRDEVPAQPSTA"
     gene            complement(1475..>4098)
                     /locus_tag="AC159P_09925"
     CDS             complement(1475..>4098)
                     /locus_tag="AC159P_09925"
                     /inference="COORDINATES: protein motif:HMM:NF036531.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0010951 - negative regulation of
                     endopeptidase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HIR3695810.1"
                     /translation="DAHLLVTTELDNVLAVRQLHVAGRQATFDIPIEPRFAPNVTISV
                     TYVRGSEMFTQQLDLKVPARDKLLNLQIIPNKKEFRPRETASYTVLARNADGSPAAGA
                     EVSLGVVDESIYSIAPESVGDIRRTFYDRRYNQVQTSFSVNYYFIGYAGKKVINLAAK
                     NKRRQFADTKNEGETVNPLIRKIFKDTAFWQPALVTGPDGKATAKFELPDNLTTWRAT
                     ARAVTSDTRVGTAVSRVVERKDVIVRVALPRFLTAGDTVTLSGIAHNYLKQEKVTKIN
                     IDVQGARLLDAPEQTVSIPSFGEHRVNWRVSVPATGELKVLVKALTDAESDAVEIAIP
                     VVPRGLKVTRAESAVFSEDEAEKSFNFNLPANADQNTRTFKVEVTPTVAGSLFGALDY
                     LTGYPYGCVEQTMSRFLPTVIVSQTLQNVESATVRDTNDIGRKVRRGLRRLYNFQHQD
                     GGWGWWKDDETDPWMTAYVVDGLAQARAAGYEVDADRLERARKRLAAMISAGQTGSNS
                     AFAEDSRAYMTYALAISGGADARFVNDTFNRRDKLGAYGRALLALTLREAGDTKRAAV
                     VASDIERTAKANASEANWETVEATALSVKALARLAPKSELLPKAARWLVRSRKYGHYW
                     QSTRETAFAIFGLTDYLKVSQELSPDYAVEVYVNGQQVLAQQMTSAEATAAKVFTIQR
                     RGGEVGGANEVRVVKRGPGVLYLSTTLTHHTNDENTAAQGVPQLKLSREYLRLSVVDK
                     GEGGLGWKVEPLSGDVRSGDIIVSRLKVEGEKSLSYLMVEDPIPAGCEPVEEVSGINL
                     HHTEEGWSDWYSAREFRDQRAVLFLNYFDGSAQFQYAMRVQVPGQFRIAPARVEQMYA
                     PDVRANSASGAMTILDK"
BASE COUNT          609 a         1374 c         1429 g          686 t
ORIGIN      
        1 ggcggggccg acgccgcccc cgcacttgtg acgcttctgc acgtaaggct tcccttgctg
       61 cgcgggcttg ccgtcttccg ctctgagccg cctgacgttc ttcgcgtaga ggtcttcctc
      121 gccgtccctg acgggctggc gcagcgcgat gctctcgaag acgtactcct tcgccttccc
      181 gtccggcgtc ttcaggtcgc gcttcagccg ctcctcgcgc accgtctcgg tgaaggcgat
      241 gctgaacagc cccgcctgat agcgcccgac gctctcgccg agcttttcaa ggacgcgcgc
      301 cagccgcacg gccttctcgt cgccttgatg cggcgctccc gcccgcggct cctgaccgag
      361 tgcgacggcg ctcagcaagc tcgccaacaa gagaccgacg acgacgaaca gcactctttt
      421 catcatggct tcggcccatg tcgttgaatg tcacgcacgg gggcgtgccg ctacgcggtg
      481 ctcggctgcg cggggacttc gtcgcgcggc gggtcggcga cgggcggcgc ttcgcccgcc
      541 gtcgagacct cgccgccggg cgcgagcttc gccagcgcgc cgagcgtgac agcccagccg
      601 cagagcgtga gcaggaaggc gagcgcgagg cgcagcccga agagtatgag ttcgccccag
      661 ccgttcttga cgggcgtggt ggtgtagacg acgaagtagg gcatgaagcc gaagaggaag
      721 aggccgaccg cgtagacgag caccgcgcgc ggcgcgtagg cgcgggccag ccagcggtgc
      781 gcgttccgga gcgccgccac gagtccgtcg cgcgcgacgg cgagccagag gtggatgccc
      841 gcgagcggga gcaccacgcc gagcagcagg agccgcaacg cggagaaggt cacgtcctgc
      901 cagcgcagag ggagcggcgg cggagcggcc ctcggccccg cgcccggcgg gacgaagcgc
      961 gcggcgtctt ccgccgtcac cgggaagcgc gcttgcagct tgttcagcag gtagtagacg
     1021 agcacggcca gcgcgatgag cggcaggctg acgagcagta tcttccagaa gtcgctgagg
     1081 gcgcgcagca ggacttcgcg cgcgcgctcg gcgcccgccg cgtggttcgc cgtcgccgcc
     1141 tgcaacagga agaacagcag cggcgcgaac ccggcgagca gcgccgacag cgcgagctgc
     1201 cccgccgtcg cgacgcccgt cgcgaagaac cagtagaccg cgaagagcag cgcgccgtag
     1261 agcgcggcga gcaccgcgag cgcgccccaa tcccgcaaca gctcgcgcgt cgcgccgccg
     1321 atggccgtga atgcgtcttt gaacatcttc cgttcccttc agcggattgc ggattgcgga
     1381 ttgcggatta gcgtgccttt catgcttgaa gttcgatgat gaacattcaa gccgcggcac
     1441 cctactaatc cgcaatccgc aatccgcaat cggctcactt gtcaagaatc gtcatcgcgc
     1501 cgctcgccga gttggcgcgc acgtcgggcg cgtacatctg ctcgacgcgc gcgggggcga
     1561 tgcggaactg gccgggcacc tgcacgcgca tggcgtactg gaactgggcc gagccgtcga
     1621 agtagttgag gaagagcacg gcgcgctggt cgcggaactc gcgcgcgctg taccagtcgc
     1681 tccagccctc ctccgtgtgg tgcaggttga tgccgctcac ctcctcgacc ggctcgcacc
     1741 ccgccgggat cgggtcttcg accatcaggt agctgaggct cttctcgccc tcgaccttga
     1801 ggcgcgagac gatgatgtcg cccgagcgca cgtcgcccga cagcggctcg accttccagc
     1861 cgaggccgcc ctcgccctta tcgacgacgc tcaggcgcag gtactcgcgc gagagcttga
     1921 gctgcggcac cccttgagcc gccgtgttct cgtcgttcgt gtggtgcgtc agcgtggtcg
     1981 aaaggtagag cacgccgggg ccgcgcttga cgacgcgcac ctcgttcgcg ccgccgacct
     2041 cgccccctct gcgctggatg gtgaagacct tcgccgcggt ggcctcggcg gaggtcatct
     2101 gctgcgcgag cacctgctgg ccgttgacgt agacctcgac ggcgtagtcg ggcgaaagct
     2161 cctgactgac tttcagatag tccgtgaggc cgaagatggc gaaggccgtc tcgcgcgtcg
     2221 attgccagta gtggccgtac ttgcgcgagc ggacgagcca gcgcgcggcc ttcggcagca
     2281 gctcgctctt gggcgcgagg cgcgcgagcg ccttcaccga aagcgccgtc gcctcgaccg
     2341 tctcccagtt ggcctccgat gcattcgcct tcgccgtgcg ctcgatgtcc gaggcgacca
     2401 ccgcggcgcg cttcgtgtcg cccgcctcgc gcagcgtcaa tgccaacaac gcgcgaccgt
     2461 aagcgccgag cttgtcgcgg cggttgaagg tgtcgttgac gaagcgcgcg tcggcgccac
     2521 ccgagatggc gagcgcgtac gtcatgtagg cgcgcgagtc ctctgcgaac gcagagttgc
     2581 tgccggtctg ccccgccgag atcatcgcgg ccagtcgctt gcgcgcgcgt tcgagccggt
     2641 cggcatccac ctcgtagccc gcggcccgcg cctgcgccag cccgtccacg acgtaggcgg
     2701 tcatccacgg gtcggtctcg tcgtccttcc accagcccca gccgccgtcc tgatgctgga
     2761 agttgtagag acggcgcagg ccgcgccgca ctttgcgacc gatgtcgttc gtgtctctga
     2821 cggtcgcgct ctcgacgttc tgcaaggtct gcgagacgat gaccgtcggc aggaagcgcg
     2881 acatcgtctg ctcgacgcag ccgtaggggt agcccgtcag gtagtcgagc gcgccgaaga
     2941 gcgagccggc gaccgtgggc gtgacctcga ccttgaacgt gcgcgtgttc tggtcggcgt
     3001 tcgccgggag gttgaagttg aaactcttct cggcctcgtc ctcggagaag accgccgact
     3061 cggcgcgcgt tactttcagg ccgcgcggga cgacggggat cgcaatctcg acggcgtccg
     3121 actcggcgtc ggtcaacgcc ttgacgagca ccttcagctc gccggtggcg ggcaccgaga
     3181 cgcgccagtt gacgcggtgc tcgccgaagc tcgggatcga gacggtctgc tccggcgcgt
     3241 cgagcaggcg cgcgccctgc acgtcgatgt tgatcttcgt caccttctcc tgcttgaggt
     3301 agttgtgcgc gatgccggag agcgtgacgg tatcgcccgc cgtcaggaag cgcggcagcg
     3361 cgacgcgaac gatgacatcc ttgcgttcga cgacgcgcga gacggcggtg ccgacgcgcg
     3421 tgtccgaggt gacggcgcgg gcggtggcgc gccacgtcgt caggttgtcg ggcagctcga
     3481 atttggccgt cgccttgccg tcggggccgg tgacgagcgc gggctgccag aaggccgtgt
     3541 ccttgaagat ttttcggatc agcgggttga cggtctcgcc ctcgttcttc gtgtcggcga
     3601 actgccgccg cttattcttc gcggcgaggt tgatgacctt cttgcccgcg tagccgatga
     3661 agtagtagtt gaccgagaag gaggtctgca cctgattgta gcgccggtcg tagaaggtgc
     3721 ggcggatgtc gccgaccgac tcgggcgcga tgctgtagat ggactcgtcc acgacgccca
     3781 gagagacctc cgcgcccgcc gcggggctgc cgtcggcgtt gcgcgcgagc acggtgtagg
     3841 aggcggtctc gcgcgggcgg aactccttct tgttcgggat gatctgaagg ttgagcagct
     3901 tgtcgcgcgc cggcaccttc aggtctagct gctgcgtgaa catctcgctg ccgcggacgt
     3961 aggtgacgct gatggtgacg ttcggggcga agcgcggctc gatggggatg tcgaaggtcg
     4021 cctgccggcc ggcgacgtgg agctggcgca cggcgaggac gttgtcgagc tcggtcgtca
     4081 ccagcaggtg cgcgtcct
//
LOCUS       DBNGOH010000506         4089 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4473557, whole genome shotgun sequence.
ACCESSION   DBNGOH010000506 DBNGOH010000000
VERSION     DBNGOH010000506.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4089)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4089)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4089
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4473557"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="AC159P_09930"
     CDS             <1..342
                     /locus_tag="AC159P_09930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695811.1"
                     /translation="LVAAMLDTKPRALADYPTGAFLWSPSSRFLVSAGPTVKLWRANG
                     ELVQELEGNAMLGATLSRDGRFLAAAHLPDYPFNLKVILKAMVGKLPKEMPIKTNVWQ
                     IDEDAVAVSAK"
     gene            complement(376..633)
                     /locus_tag="AC159P_09935"
     CDS             complement(376..633)
                     /locus_tag="AC159P_09935"
                     /inference="COORDINATES: protein motif:HMM:NF037832.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HIR3695812.1"
                     /translation="MENRVLFTLDRGFADVDKYPRGTHAGVVVFTQNRGEPIHKIEKR
                     LLGFARATRQEKLEGRTAVVERTRVRFPRGKRKGKREHPQT"
     gene            complement(695..973)
                     /locus_tag="AC159P_09940"
     CDS             complement(695..973)
                     /locus_tag="AC159P_09940"
                     /inference="COORDINATES: protein motif:HMM:NF016167.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HIR3695813.1"
                     /translation="MPAKKVLEAQIREAAAATSLTESRLRRLLGRVTVDPLQCHGEPC
                     IRGMRLMVRTIVDSLRGGMTYDDILLRYPDLTREDIDAAAAYVSLYEN"
     gene            complement(1041..2993)
                     /locus_tag="AC159P_09945"
     CDS             complement(1041..2993)
                     /locus_tag="AC159P_09945"
                     /EC_number="4.1.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF008907.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional oxoglutarate
                     decarboxylase/oxoglutarate dehydrogenase thiamine
                     pyrophosphate-binding subunit/dihydrolipoyllysine-residue
                     succinyltransferase subunit"
                     /protein_id="HIR3695814.1"
                     /translation="MRISISPNPSHLEFVDPVVEGMVRAKQDAMQIDAGRPREDVIDR
                     ALPVLLHGDAAFAGQGIVMETLQLADLKGYRTGGTLHIIINNQIGFTTSPDSARSSIY
                     STDVAKTTQLPIFHINGDDPEAAFRALRIALDFRQDFNKDVVLDVIGFRKLGHNEGDE
                     PSYTQPLMYARVKAHPGVRAKYAARLVAEGVLNAEDVEAMIKEATRRYEATLARAKEI
                     AREKPAREALPPAVEEEDGSAVVETPVAAETIRDIARKIAVVPEGFSINPKMVSQLAR
                     RVKMGEGEAPLDWAFAELVAFGSLGLEGVRVRLSGQDSGRGTFSQRHAIMYDTQTGKP
                     WSPLTEMTAVSEERARVEVFDSSLSEQGVMGFEYGYSVVARDALVAWEAQFGDFGNGA
                     QVIIDQFIAPGVDKWQQPSRLVLLLPHGFEGQGPEHSSARLERYLQLCAENNMQVCYP
                     TTPAQHFHMLRRQVRQRDARPLVVMTPKSLLRLPAAVSTIEELTSGGFRPVLDDAEAH
                     DRAAVRRIVLCSGKLFYDLAAARKKSGDAHASIVRLEQFYPFPERLLRETFASYPNAT
                     QLVWAQEEPKNMGGWTFVGPRLEGLMPKGERPYYVGRAASASPATGSYAIHELEQRQL
                     VDHALTTDAPFISNASTAQYAGQADS"
     gene            complement(3003..>4089)
                     /locus_tag="AC159P_09950"
     CDS             complement(3003..>4089)
                     /locus_tag="AC159P_09950"
                     /inference="COORDINATES: protein motif:HMM:NF012424.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HIR3695815.1"
                     /translation="AGKLGVPDFQDTTISLTNPGTIGTVSSTPRLMTGQSAIIATGAI
                     EYPAEYAAMTPQALSQLGISKTLNISSTYDHRIIQGAESGSFLAHVHALLVGEHDFYT
                     RIFRDLGIPYQPMRWAKDFNPSLLGGDHARQHVEKEARVLELVNAYRVRGHLIADIDP
                     LNALPVQYHPELDIETYRLTIWDHDREFITGGLAGRESATLREILDILRRAYCGKVGT
                     EYRHIQSKEQKTWIRDRIRAEFVEPQPLPVETRKQLLSRLTYAEQFERFLHTKYLGQK
                     RFSLEGCETIIALLDQLVERAPDQGVEDITLGMAHRGRLNVLANVVGDFAERIFTAFE
                     GSVHPSFPADEGDVKYHQGAEGERTTA"
BASE COUNT          622 a         1312 c         1407 g          748 t
ORIGIN      
        1 ctcgtcgcgg cgatgctcga caccaagccg cgcgccctgg ccgactaccc gaccggcgct
       61 ttcctgtgga gtccgagcag ccggttcctc gtcagcgccg gcccgaccgt caagctctgg
      121 cgcgcgaacg gcgagctggt gcaggaactc gaaggcaacg cgatgctcgg ggcgaccctc
      181 agccgcgacg gcaggttcct cgcggcggcc catctgccgg actacccctt caacctcaag
      241 gtcatcctca aggccatggt cggcaagctg ccgaaagaga tgccgataaa aaccaacgtc
      301 tggcagatcg acgaagacgc ggtcgccgtc tcggccaagt aagaaccgtc gcccctgctc
      361 ccgcgatgta ttgcattacg tctgaggatg ttctctcttc cccttacgtt tcccgcgcgg
      421 gaagcgcact cgcgtcctct ccactaccgc cgtgcgaccc tctagctttt cctgccgggt
      481 agcgcgcgcg aagccgagca atcgcttttc aatcttgtgt attggttcgc cgcgattctg
      541 cgtgaacaca acaaccccgg cgtgagtacc acgcgggtat ttatccacgt cggcgaaacc
      601 tctgtcgagc gtgaagagaa ctcgattttc cagcgtcgct taaaaagcaa gcacggcatc
      661 gctcgccttc ctcaatcctt cctctgctgc tgttttaatt ttcatacagg ctgacgtaag
      721 cggctgcggc atctatatct tcccttgtga ggtcagggta gcggagtaag atgtcatcgt
      781 acgtcatccc gcccctcaag gaatcgacga tggtgcgcac catgagccgc atccccctga
      841 tgcacggctc accatgacac tgaagcgggt caaccgtcac gcgccccagc agacgccgta
      901 accgactctc agtcaggctg gtcgcggcgg cagcttccct gatttgcgct tccagcacct
      961 tcttagcagg cataccgttg acctctcatt caggcacgat gttgggccgc ccgcggttta
     1021 ccatcccccg ccgagattgt tcaggagtcg gcctgcccgg cgtactgcgc ggtgctcgcg
     1081 ttggagatga agggggcgtc cgtcgtgagc gcgtgatcga cgagctggcg ctgttcgagt
     1141 tcgtggatgg cgtaagagcc tgtggcgggg ctggccgagg ccgcgcgccc gacgtagtag
     1201 gggcgctcgc ccttcggcat cagcccttcg aggcgcggcc cgacgaacgt ccagccgccc
     1261 atgttcttcg gctcctcctg cgcccagacg agctgcgtcg cgttcgggta cgaggcgaac
     1321 gtctcgcgca aaagccgttc ggggaacggg tagaactgtt cgaggcggac gattgatgcg
     1381 tgcgcgtcgc ccgacttctt gcgcgcggcg gccagatcgt agaagagctt gccgctacag
     1441 aggacgatgc ggcgcacggc ggcgcgatcg tgcgcctctg cgtcgtcgag gacggggcgg
     1501 aagccgcccg aggtcaactc ttcgatggtc gagacggcgg cgggcaggcg caacaggctc
     1561 ttcggcgtca tgacgacgag cgggcgcgcg tccctttgcc tgacctgccg gcggagcatg
     1621 tggaagtgct gtgccggggt ggtcgggtag cagacctgca tgttgttctc ggcgcagagc
     1681 tggaggtagc gttcgagccg cgccgaggag tgctccggcc cctgcccctc gaagccgtgc
     1741 ggcagcagca gcacgagccg cgagggctgc tgccacttgt ccacgccggg cgcgatgaac
     1801 tggtcgatga tgacctgcgc gccgttcccg aagtcgccga actgcgcctc ccacgccacc
     1861 agcgcgtcgc gcgcgacgac cgagtagccg tactcgaagc ccatcacgcc ctgctcggac
     1921 aaggagctgt cgaatacctc gacgcgcgcg cgctcttcgg agacggcggt catctcggtg
     1981 agcggcgacc agggcttgcc ggtctgcgtg tcgtacatga tggcgtggcg ctgcgagaag
     2041 gtgccgcggc ccgagtcctg ccccgagagg cggacgcgca cgccttcgag gccgagcgag
     2101 ccgaacgcga ccagctccgc gaaggcccag tccaacggcg cctcgccctc gcccatcttc
     2161 acgcggcgcg cgagctggct gaccatcttc gggttgatgc tgaagccctc gggcacgacc
     2221 gcgatcttgc gcgcgatgtc gcggatcgtc tccgccgcga ccggcgtctc gaccaccgcc
     2281 gagccgtcct cctcctcgac ggcgggcggc agcgcctcgc gcgcgggctt ctcgcgggcg
     2341 atctccttgg cgcgcgcgag cgtcgcctcg tagcgccggg tcgcctcctt gatcatcgcc
     2401 tccacgtctt ctgcgttgag cacgccctcg gcgacgaggc gcgcggcgta cttggcgcgc
     2461 acgcccggat gcgccttgac gcgcgcgtac atgaggggct gcgtgtagct cggctcgtcg
     2521 ccctcgttgt ggccgagctt gcggaagccg atcacgtcga gcaccacgtc cttgttgaag
     2581 tcctgccgga agtcgagcgc gatgcgcagg gcgcggaagg cggcctcggg gtcgtcgccg
     2641 ttgatgtgga agatggggag ctgcgtggtc ttcgccacgt cggtcgagta gatcgaggag
     2701 cgcgccgagt cgggcgaggt cgtgaagccg atctggttgt tgatgatgat gtggagggtg
     2761 ccgcccgtcc ggtagccctt gaggtccgcg agctgcaagg tctccatcac tatcccttgc
     2821 ccggcgaagg ccgcgtcgcc gtggagcagc acgggcaggg cgcggtcgat cacgtcttcg
     2881 cgcgggcggc ccgcgtcgat ctgcatcgcg tcctgtttgg cgcgcaccat gccttcgacg
     2941 acggggtcca cgaattcgag gtggctcggg ttgggcgaga tcgagatgcg caccttcttg
     3001 ccctaggcgg tggtgcgctc gccctccgcg ccctgatggt acttcacgtc gccctcgtcc
     3061 gccgggaagc tcgggtggac ggagccttcg aaggccgtga agatgcgctc ggcgaagtcg
     3121 ccgacgacgt tggccaggac gttcagcctg ccgcggtgcg ccatgccgag cgtgatgtct
     3181 tcgacgccct gatcgggcgc gcgctcgacc agttggtcga ggagcgcgat gatcgtctcg
     3241 cagccttcga gcgagaagcg tttctgcccg aggtacttcg tgtggaggaa gcgctcgaac
     3301 tgctcggcgt aggtgaggcg cgagagcagt tgcttgcggg tctccacggg gagcggctgc
     3361 ggctcgacga actcggcgcg gatgcggtca cgaatccacg tcttctgctc cttgctctgg
     3421 atgtgccgat actccgtgcc gaccttgccg cagtaggcgc ggcgcaggat gtcgagtatc
     3481 tctctcaagg tcgcgctctc gcggcccgcg agtccgccgg tgatgaactc gcggtcgtgg
     3541 tcccagatcg tcagccggta ggtctcgatg tcgagttcgg ggtggtactg gacggggagc
     3601 gcgttcaagg ggtcgatgtc cgcgatgagg tggccgcgca cgcggtaggc gttgacgagt
     3661 tcgaggacgc gcgcctcctt ctcgacgtgc tggcgcgcgt ggtcgccgcc gagcaaggaa
     3721 gggttgaagt ccttcgccca gcgcatcggc tggtacggga tgccgaggtc gcggaagatg
     3781 cgcgtgtaga agtcgtgctc gccgacgagc agcgcgtgga cgtgggcgag gaacgagccg
     3841 gactccgcgc cctggatgat gcggtggtcg tacgtcgaag agatgttgag cgtcttgctg
     3901 atgccgagct gcgacagagc ctgcggcgtc atcgcggcgt actcggcagg gtactcgatg
     3961 gcgccggtgg cgatgatggc cgactgaccc gtcatcaggc gcggcgtcga ggagacggtg
     4021 ccgatggtgc cggggttagt caggctgatg gtcgtgtcct gaaagtcggg gacgccgagc
     4081 ttgccggcg
//
LOCUS       DBNGOH010000507         2886 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2274045, whole genome shotgun sequence.
ACCESSION   DBNGOH010000507 DBNGOH010000000
VERSION     DBNGOH010000507.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2886)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2886)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2886
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2274045"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            183..1343
                     /locus_tag="AC159P_09955"
     CDS             183..1343
                     /locus_tag="AC159P_09955"
                     /inference="COORDINATES: protein motif:HMM:TIGR01910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HIR3695816.1"
                     /translation="MRDLIAIPSVNPMRPCTGEAVERDVADYIETVLRRAGVDCVRQT
                     VFEGRENVIGVVHAGGSGEGSRRGLMFNSHMDTVPVANMSIDPFDPVVRDGRVYGRGA
                     CDAKGPIAAMLAAVTAYAGRRERPATIVFAAMADEEFAFSGAWKLIEREWPVAACVVG
                     EPTRLALIVAHKGVARWRVKVRGRSAHGATPQLGRSAIYDGARVCLALEAYARELAAR
                     EPHTLLGAPSLNVGRVAGGQSVNIVPDLCEFEMERRLLPGEDGRAAVGDCEARVRAEA
                     GEGVDLEFEEPYLVDPALDTPPEAAVASAVRRAHLEEFGADCEVGGAHYGTDGSKLAR
                     AGIETVVCGPGDVAQAHTKDEYVEVEQLERAVRLYARVIEGWPGAAPAGGGR"
     gene            1343..1894
                     /locus_tag="AC159P_09960"
     CDS             1343..1894
                     /locus_tag="AC159P_09960"
                     /inference="COORDINATES: protein motif:HMM:NF012514.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HIR3695817.1"
                     /translation="MVRKKGPWTVEETAAVYRTPWVEVVEEKVVRPDGTRGLFSTVLM
                     RPGVSVLALDEEGFVHLTSEYRYAVERESVEVVSGGVDDGETPLDAARRELREELGIA
                     AREWTDLGPVDPFTSVVLSPARLFLARGLTHASHERETTEVIRALRVLLPDAVRMVMA
                     SEITHGPSCVLILKAHLYLSNGG"
     gene            1884..2636
                     /locus_tag="AC159P_09965"
     CDS             1884..2636
                     /locus_tag="AC159P_09965"
                     /inference="COORDINATES: protein motif:HMM:NF027338.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding family 9-like protein"
                     /protein_id="HIR3695818.1"
                     /translation="MGAEFERGLLALAAAFVCTASCAASAQESARPLPTYEVLRASSP
                     VKVDGRLDEAAWAAARGVGPFVRNRDGAAVPNGTEAKVLYDERFLYFSFRVEDENVWA
                     TMRRRDQHLWHEEVVEVFLQADTRVPNYIELEVNPLGALLDIYLLDRRKNLPYESWNS
                     AGVKWAVRVDGRVDGKPGDRGWTCEIALPLEDVATAPRLPPRPGDRWRMNLYRVESRP
                     EPLGLAWSPTLEGDFHVPPMFGEIIFGGRAVP"
     gene            2672..>2886
                     /locus_tag="AC159P_09970"
     CDS             2672..>2886
                     /locus_tag="AC159P_09970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HIR3695819.1"
                     /translation="MKNRLWLIYSLVTTAFWGVWGALIELPEKAGFPATLSYAVWALT
                     MIPPALVALKIIGWKLERDRRSIILGLL"
BASE COUNT          427 a          948 c         1081 g          430 t
ORIGIN      
        1 cgacgcctga gccttcacat ccgttcaggc gtcgctgacg cgacgcggcc atgtgtggtt
       61 cgacttcaac ccggccatga aaggccgggc taaactcaga cgccgctgac gcggcggaat
      121 cgtgcgcact tcgaacttag ttgacgggta aagaaccgat ggccgtcgaa gtcttacaga
      181 cgttgcgcga cctgatcgcg attccgagcg tcaacccgat gcggccgtgt acgggcgagg
      241 cggtcgagcg agatgtcgcc gactacatcg agacggtctt gcggcgcgcg ggtgtagact
      301 gcgtgcggca gaccgtcttc gaggggcgcg agaacgtcat cggcgtcgtc cacgcgggcg
      361 gttccggcga aggctcgcgc cgcggcctca tgttcaactc gcacatggac acggtgccgg
      421 tcgccaacat gtccatcgac ccgttcgacc ccgtcgtgcg cgacggtcgc gtctacgggc
      481 gcggcgcgtg cgacgccaaa ggccccatcg ccgcgatgct cgccgccgtc accgcctacg
      541 ccgggaggcg cgagcgtccc gccacgattg tcttcgccgc gatggccgac gaggagttcg
      601 ccttctcggg cgcgtggaag ctcatcgagc gcgaatggcc cgtggccgcg tgcgtcgttg
      661 gcgagccgac acggctcgcg ctcatcgtcg cgcacaaggg cgtggcgcgc tggcgcgtga
      721 aggtcagggg ccggagcgca cacggcgcga cgccgcagct cggtcgcagc gccatctacg
      781 atggcgcgcg cgtctgcctc gccctcgaag cctacgcgcg tgagcttgcg gcgcgcgagc
      841 cgcacacgct tctcggggca ccctcgctca acgtcgggcg cgtcgcgggc gggcagtcgg
      901 tcaacatcgt gcccgacctg tgcgagttcg agatggagcg gcgtctgctg ccgggcgagg
      961 acggtcgcgc tgccgtcggc gactgcgagg cgcgcgtgcg tgcagaggcg ggcgaagggg
     1021 tcgatcttga gttcgaggag ccttacctcg tagaccccgc gctcgacacg ccgcccgagg
     1081 ccgcggtcgc gtcggcagtg cgccgcgcac acctcgaaga gttcggcgcg gactgcgagg
     1141 tcggcggcgc acactacggg acggacggaa gcaagctggc gcgcgccggg atcgagacgg
     1201 tcgtctgtgg gccgggcgac gtggcgcagg cgcacacgaa ggacgagtac gtcgaggtcg
     1261 agcagttgga gcgcgccgtc cgcctctacg cgcgcgtcat cgaaggctgg ccgggcgccg
     1321 cgcccgcggg aggaggccgc tagtggtcag gaagaaaggc ccgtggacgg tggaggagac
     1381 cgccgcggtc taccgcacgc cgtgggtcga ggtcgtcgag gagaaggtcg tcaggcccga
     1441 cgggacgcgc ggcctctttt cgacggtgct catgaggccg ggcgtctccg tgctcgcgct
     1501 cgacgaagag ggcttcgtcc acctgacgag cgagtaccgc tacgccgtcg agcgcgagag
     1561 cgtcgaggtc gtgagcggcg gcgtcgatga cggcgagacc ccgctcgacg cggcgaggcg
     1621 agaactccgc gaggagctgg gcatcgccgc gcgggagtgg acagacctcg gcccggtcga
     1681 tccgttcacc tcggtcgtcc tctcgcccgc gcggctcttc ctcgcgcgcg ggctgacgca
     1741 cgcctcccac gaacgcgaga cgaccgaggt catcagggcg ctgcgcgtct tgctgcccga
     1801 cgccgtccgg atggtcatgg ccagcgagat cacgcacggg ccgagctgcg tgctcatcct
     1861 gaaggcgcac ctttacttgt cgaatggggg ctgagttcga acgggggctg ctggcgctgg
     1921 ccgccgcgtt cgtgtgcacg gcatcgtgcg cggcgtcggc gcaggagagc gcgcgcccgc
     1981 tgccgaccta cgaagtcctg cgcgcgtcgt cgccggtcaa ggttgacggc aggctggacg
     2041 aggcggcgtg ggcggcggcc cgcggcgtgg ggcctttcgt gcggaaccgg gacggcgcgg
     2101 cggtgccgaa cgggaccgag gcgaaggtgc tctacgacga acgcttcctc tacttctcct
     2161 tccgcgtcga ggacgagaac gtctgggcca cgatgcgccg gcgcgaccag cacctctggc
     2221 acgaggaggt cgtcgaggtc ttcctacagg ccgacacgcg cgtgcccaac tacatcgagc
     2281 tggaggtgaa cccgctgggg gcgctgctcg acatctacct gctcgaccgg cgcaagaacc
     2341 tgccctacga gagctggaac agcgccggcg tgaagtgggc cgtgcgcgtg gacgggcgcg
     2401 tggacgggaa gccgggcgac cgcggctgga cgtgcgagat cgcgctgccg ctcgaagacg
     2461 tggcgacggc gccgcgcctg ccgccgcgcc ccggcgaccg ctggcgcatg aacctctacc
     2521 gcgtcgagag caggcccgag ccgctggggc tggcctggtc gccgacgctt gagggagact
     2581 tccatgtccc gccgatgttc ggcgagataa tcttcggcgg acgggcggtg ccgtaaacgt
     2641 cgtgcggctc gcgggggtcg cgggggtgat aatgaagaac aggctgtggc tgatctactc
     2701 gctggtgacg acggcgttct ggggcgtgtg gggcgcgctc atcgagcttc cggagaaggc
     2761 gggcttcccc gccacgctca gctacgcggt gtgggcgctg acgatgattc cgcccgcgct
     2821 ggtggccttg aagatcatcg ggtggaagct cgaacgcgac cgccgctcga tcatcctcgg
     2881 cctgct
//
LOCUS       DBNGOH010000508         1712 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1176094, whole genome shotgun sequence.
ACCESSION   DBNGOH010000508 DBNGOH010000000
VERSION     DBNGOH010000508.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1712)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1712)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1712
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1176094"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..591)
                     /locus_tag="AC159P_09975"
     CDS             complement(<1..591)
                     /locus_tag="AC159P_09975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695820.1"
                     /translation="MRLKLYCNRYAHFKDALVCSVSCVYRTRCHDFALFYDGHREDVD
                     ALVADYYAGRREGERKEAERPSASSRQLPLAPVAPTVLARPEELRNLIRLEVKRKMTE
                     AYYIWVDREDRAELLDTAEVLKRAERGHKAKSIYRVSQEMELRYQLVPRKRIEKAKRV
                     AAEDAERAEGRRTRRASSRPAKQEPPPPVPFPAPVAV"
     gene            926..1444
                     /locus_tag="AC159P_09980"
     CDS             926..1444
                     /locus_tag="AC159P_09980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695821.1"
                     /translation="MRVRMYKPNFCAECGERVARERWRVWTSRRFCQLCDVRFRRRRF
                     AASLFACAALVAAGFAAGRLARPAAPPPPLFIERGGAALVPLPADSATRRLDGQPRQP
                     RPADEPRHGPDGTQDERPTEPDEVVSVCGARTQKGTPCQRRVRGTGRCWQHRGKPAML
                     PPAKLIVSSEQE"
BASE COUNT          253 a          617 c          554 g          288 t
ORIGIN      
        1 gacggcgacg ggcgcgggga aggggacggg cgggggcggc tcctgtttgg cgggtcggct
       61 gctcgcgcgg cgtgtgcggc gcccctcggc gcgctcggcg tcttcggcgg cgacgcgctt
      121 cgccttctcg atgcgcttgc gcggcacgag ctggtagcgc agctccatct cctgcgagac
      181 gcggtagatg ctcttggcct tgtggccgcg ctcggcgcgc ttcagcacct ccgccgtgtc
      241 cagcagctcg gcgcggtctt ccctatcgac ccagatgtaa tacgcttcgg tcatcttcct
      301 cttaacctcc agacgaatca gattacgcag ctcttccggc ctcgcgagca ccgtcggcgc
      361 gaccggcgcg agcggaagct gccgcgacga cgcggaaggc cgctcggcct cctttcgctc
      421 gccttcgcgc cgcccggcgt agtagtccgc gacgagcgcg tccacatcct cgcggtgccc
      481 gtcgtagaag agcgcgaagt catggcagcg cgtgcggtac acgcagctca ccgagcacac
      541 gagcgcgtcc ttaaaatgcg cgtagcggtt acagtagagt ttcagacgca tcggacttcg
      601 aggcaccgtg ggacgcaagg agcgggctgg ccgccgaggg cctgcgcttc gcgcgccccc
      661 gcacgcttcg ggagcctgcg aactatatca cgcctgttcc gcgcgtgcaa cgcgagcctg
      721 cgcatgaaac acacgccgcg tcatcgaccg cgagcgcctg tgccttgcgc gcacgtgtcg
      781 cgcgcgtata atgcgcgtgc gggcgagttg cccgaccttc cgcgtccgat tctctcaacc
      841 tacaattttc cggtcgcgcg cgccgccccg ctctcgacca agtctctccg acgacgcccg
      901 cgcgggcgtc ctcacaggga aggagatgcg cgttcggatg tacaagccca atttctgcgc
      961 cgagtgcggc gagcgcgtcg cgcgcgagcg ctggcgcgtg tggacgagcc gacgcttctg
     1021 ccaactgtgc gacgtgcgct tccgccgccg gcgcttcgcc gcgtcgctct tcgcctgcgc
     1081 ggcgctcgtc gccgcgggct tcgccgcggg acgactcgcg cgacccgccg cgccgccccc
     1141 ccctctcttc atcgagcgcg gcggcgcggc actcgtcccg ctccccgccg acagcgcgac
     1201 gcggcgactc gacggacagc ctcgacaacc gcgccccgcg gacgagccgc gccacggccc
     1261 cgacggcaca caggacgagc gcccgaccga acccgacgag gtcgtctccg tctgcggcgc
     1321 gcgcacgcag aagggcacgc cctgccagcg ccgcgtccgc ggcacgggcc gctgctggca
     1381 gcaccgcggc aagcccgcga tgctgccgcc cgcgaaactg atcgtgagca gtgagcagga
     1441 gtaggcagta ggcagtaggc ggtaggcagt caagacagga gggcgaggat gcttgaggtc
     1501 gaaagcctca agcatcctcc tcgtactgtt tgacgaacga agatgcttga gaggtaaagt
     1561 ccagagccac ttcgttattc cgaacccctg ccccggactc cagtctcgtg atgggcgcga
     1621 ccgacttctg acttctgact tctgacttct gacttctgac ctctgacttc tgtattctgt
     1681 tttaactgcc tactgcctgc tcctgctcac tg
//
LOCUS       DBNGOH010000509         7553 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3925605, whole genome shotgun sequence.
ACCESSION   DBNGOH010000509 DBNGOH010000000
VERSION     DBNGOH010000509.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7553)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7553)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7553
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3925605"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            485..3898
                     /locus_tag="AC159P_09985"
     CDS             485..3898
                     /locus_tag="AC159P_09985"
                     /inference="COORDINATES: protein motif:HMM:NF051698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR3695822.1"
                     /translation="MCCFAVGTQRVAAQSEASTGQIAGSVKDPNDAAVPNASVTVTNT
                     QTGLQRTATTSEDGLYTLVLLPPGTYTVAAQGGNFSETRLENVVVQVGRVADGNIVLG
                     VSAVQETVDVTASTEAVQVTRNESDAVLNQTAINTLPINGRRFQDFVTLTPTAQVDPQ
                     RGQISLSGQKGINSNINVDGVDYNQPFFGGIRGGERANLAFTLPQEAIKEFQVVASGY
                     SAEFGRSTGGVVNAVTKSGNNELHGSAFYLLRPSRLARGNEFTEELQEQNLSARGVDA
                     SLAPTQHQFGGSIGGPIVKNKLFYFGAYEQQKFNADRVIIFNLAANQTAANAGQQAVL
                     DFFRAQQTSYELTNDAFAGLARVDWNVNNSNQFNARFSASKNEAINAVSRGETSLDPT
                     TTQALTTNGTEENKNKIFVSQLVSTIGSGAVNEFRFQFAREDRPRLSNSELPQLLTSF
                     ATIGATAFLPTTQYDRRVQFADSFTLLTGNHNIKFGGEFSDLYANQFFGFNQFGQYVY
                     STTNIPQSLGCLSNVPATAACRSGGITPFLGRFDDNVGTGTARYLKQLGNLQADFKVK
                     ELAFFAQDSWRLDPQLTVNYGIRFEQQYNPEADTSNTALANVVRNTVFPIRGAGYNPT
                     IPDSGWQVGPRIGFAYDPSGEGKTVIRGFAGLYYARTPLIVFADSTNNYRATPANVST
                     QLPFSGFNQTTFNTFAASAAGAQYRAITGCDPAAATGTPARNACTPSTLFRQFAIAGV
                     NLNSFALDNLPILSTEQISAIAGGLGLNPNPFVGAQVTGHTEDFKNPRSAQFGFAFEH
                     ELARGFIVGVDFSHVHTDRIQRNRDLNLPAPLSAEEYINFLRANNTAANFAAMEASGT
                     IATIRQSGRSYIAVNTPAALTFPANSVTTRLRPTQAQGLLQLGAVQVRESTAVSDYDQ
                     LTFRTRLIRRRGQVNVYYTLSRSLGDDDNERDAGGVAYADAYDLSREYNFSRLDRRHQ
                     FTANPILFLPWDFEISSAIRLRSGTPLNTTVGADLNGDTVNNDRPLESPGVVFKRNAF
                     RNRAIYDIDLRVQKSVLFGETRRLSFSAEFFNLFNRSNIIFPTPNTATSSGILGQFCT
                     PTSQLCGLSGSTNPLFLQITDPNTGALLVNNTNPGSQVFQMQLGARFQF"
     gene            4015..4746
                     /locus_tag="AC159P_09990"
     CDS             4015..4746
                     /locus_tag="AC159P_09990"
                     /inference="COORDINATES: protein motif:HMM:NF018894.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HIR3695823.1"
                     /translation="MSERRRKVPRVPTAIPVEWGVTSAYAHGGRVVSLSVRGCLIRTA
                     HVEPLYGKTVHLRFPLPDDDLLALAGHVIYYLRDVGFAVEFDAPPDREHYLLARLVQD
                     YRKGDPYKAPGADALQLVARQDAPAVTFKDQRRHARAKVSINVDWGETPACEYGGDRV
                     TSISLGGCFIQTERHVREGAVAYVRLWQRPGGRGVFRGVVRYQLQLSPRHAPIGLGLE
                     FPDLDDEKTASLKEVLNFYGEALST"
     gene            5013..5225
                     /locus_tag="AC159P_09995"
     CDS             5013..5225
                     /locus_tag="AC159P_09995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019459345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HIR3695824.1"
                     /translation="MGIPNKDEVEGKFDQAKGAVKENVGRAVGDREMETEGAADRASG
                     NVQEGFGTAKRKVGDAVKDIGDAIRD"
     gene            complement(5398..5904)
                     /locus_tag="AC159P_10000"
     CDS             complement(5398..5904)
                     /locus_tag="AC159P_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017879052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695825.1"
                     /translation="MAVNKLVTGLFKTRVAAEAAVDAILKRGYTRDDISVLMSDATKS
                     KEFAIETGSRAGEGAGIGGAVGGAVGAVIAALAAVGTNIALPGLGLIVAGPLVAALAG
                     AGAGGAAGGLVGALVGAGIPEHRAKVYDASLRAGGILIGVEAKTEEEAEALEKLLTDL
                     GAEYVRQE"
     gene            6246..6941
                     /locus_tag="AC159P_10005"
     CDS             6246..6941
                     /locus_tag="AC159P_10005"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011520840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR3695826.1"
                     /translation="MIEAVIFDVDGTLVDSVDLHARAWQEAFARFGKQVPYEDVRYQI
                     GKGGDQLMPVFFSREELDDFGEEMEKLRGEHFKREYLPRVRAFPRVRELFERVRADGK
                     RIALASSAKEEELDEYKKIARIVDLVEEETSADDADKSKPHPDIFEAALAQLGSISPD
                     RVIVVGDTPYDAEAARKAGIRTIGLLSGGFPEEDLRTAGCLQIFRDAADLLARYEDSL
                     IARDPSSTATAHD"
     gene            6977..>7553
                     /locus_tag="AC159P_10010"
     CDS             6977..>7553
                     /locus_tag="AC159P_10010"
                     /inference="COORDINATES: protein motif:HMM:NF024831.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR3695827.1"
                     /translation="MRPNERNLKILHVSSARTLGGGERHLADLARGLARRGHEVYAAL
                     RTDSPLRAELLSSLPQQNIFTLPLKGALDLPSALELARLAREHEIDILHAHLARDYPP
                     AALAARRAHAARLVITRHVLFPMNRAQRLALSNAARVIAVSGGVARALRARHIFSEHK
                     IRVVPNAIDVARVEADLGGFERDSDRAETLRA"
BASE COUNT         1386 a         2836 c         2141 g         1190 t
ORIGIN      
        1 agaaatgccc gctcgacgat ttttttcgac gacggcagtc acgggaaacg gccttcgacg
       61 ccgcgaaccg gcggagggcc gccgaaagtt taccgataac aaatctgttg tttcgaaacc
      121 gcgcgcgacc tcgtcgcagc ccgcgcccgc ggcggccagg tcgggcacgc gcggaatccg
      181 ggaatgggat aaagccggac gcgactttca accttgcgcc gccggttgga aatatagtat
      241 ttaacaactt gcttacgtcg ccgtaagact cgcgcaaacg gctgcccgta tggtataagt
      301 tcggctttca aaccgagcca tacattcatt cactaccgca agctatcgaa atcatccggg
      361 ttttcaaaag ggcgcacgcg ttcctctgat cccgttacgc cacgtcaaca atctttcagc
      421 tagaaaagga agagttatgt cgaatctttc cctccgaatg tcagcctgcg tgctggtgtt
      481 cttgatgtgc tgctttgccg ttggcaccca gcgggttgcg gcccagtccg aggccagtac
      541 cgggcagatc gccgggtcgg tcaaagaccc caacgatgcc gccgtgccca atgcctccgt
      601 cacggtcacc aatactcaaa ccggacttca gcgcacggcg acgacgagcg aagacggtct
      661 ttacaccctt gttctgctcc cgccgggcac ctacacggtc gccgcccagg ggggtaactt
      721 cagcgaaact agacttgaga acgtcgtcgt tcaggtcggg cgcgtcgccg acggcaacat
      781 cgttctcggc gtcagcgccg tgcaggaaac cgtggatgtg accgcgtcca cggaagcggt
      841 gcaggtcacg cgcaacgagt cggacgccgt cctgaaccag acggcgatca acaccctgcc
      901 gatcaacggt cggcgcttcc aggacttcgt cacgctgacc ccgaccgcgc aggttgaccc
      961 gcagcgcgga cagatttccc tctccggaca gaagggcatc aactcgaaca tcaacgttga
     1021 cggcgtggac tacaaccagc ccttcttcgg cggcatccgc ggcggcgagc gcgccaatct
     1081 cgcgttcacg ctcccgcagg aagcgatcaa ggagtttcag gtcgtcgcct ccggctactc
     1141 ggctgagttc ggacgctcca cgggcggcgt cgtcaacgcc gtcacgaaat ccggcaacaa
     1201 cgagctgcac ggctcggcct tctatctgct gcgcccctcc cgcctcgcgc gcggcaacga
     1261 gttcacggag gaactccaag agcagaacct gtcggcgcgc ggcgtggacg cctcgctcgc
     1321 cccgacgcag caccagttcg gaggctctat cggcggcccc atcgtcaaga acaagctctt
     1381 ctacttcggc gcttacgagc agcagaagtt caacgccgac cgcgtgatca tcttcaacct
     1441 cgcggccaac cagaccgccg ccaacgcggg gcagcaggcc gtgctcgact tcttccgcgc
     1501 ccagcagacc agctacgagc tgacgaacga cgccttcgcc ggtctcgcgc gcgtggactg
     1561 gaacgtcaac aactcgaacc agttcaacgc gcgcttcagc gccagcaaga acgaggccat
     1621 caacgccgtc tcgcgcggcg agacctcgct cgacccgacg acgacgcaag ccctcacgac
     1681 gaacggcacg gaggagaaca agaacaagat tttcgtctcg cagctcgtca gcaccatcgg
     1741 gtcgggggcc gtcaacgagt tccgcttcca gttcgcgcgc gaagaccgcc cgcgcctgtc
     1801 gaactccgag ctaccgcagc tcctgacaag cttcgccacc atcggcgcga ccgccttcct
     1861 gccgaccacg cagtacgacc gccgcgtcca gttcgccgac agtttcaccc tcctgaccgg
     1921 caaccacaac atcaagttcg gcggcgagtt cagcgacctt tacgccaacc agtttttcgg
     1981 cttcaaccag ttcggccagt acgtctactc gaccacgaac atcccccagt cgctgggctg
     2041 cctgtcgaac gtccccgcga ccgcggcgtg ccgcagcggc ggcatcaccc ccttcctcgg
     2101 gcggttcgat gacaacgtcg gcaccggcac ggcgcgctac ctgaagcagc tcggaaacct
     2161 tcaggccgat ttcaaggtga aggagctggc cttcttcgcg caggactcgt ggcgcctcga
     2221 cccgcagctc acggtgaatt acggaatccg cttcgagcag cagtacaacc ccgaggccga
     2281 caccagcaat acggcgctcg ccaacgtcgt ccgcaacacg gtcttcccga tccgcggcgc
     2341 gggctacaac ccgacgatcc ccgacagcgg ctggcaggtc gggccgcgca tcggcttcgc
     2401 gtacgacccg agcggcgagg gcaagaccgt catccgcggc ttcgcgggtc tctactacgc
     2461 ccgcacgccg ctcatcgtct tcgccgacag caccaacaac taccgggcga cgcccgccaa
     2521 cgtcagcacg cagctaccct tcagcggctt caaccagacg accttcaaca cgttcgcggc
     2581 gtcggctgcc ggggcgcagt acagggcgat aacgggctgc gacccggcgg cggcgaccgg
     2641 cacccccgcg cgcaacgcct gcacgccgag cacgctgttc cgccagttcg ccatcgccgg
     2701 cgtcaacctc aacagcttcg cgctggacaa cctgccgatc ctctcgaccg agcagatctc
     2761 ggccatcgca gggggcctcg gcctcaaccc gaacccgttc gtcggcgcgc aggtcacggg
     2821 ccacaccgaa gacttcaaga acccgcgctc ggctcagttc ggattcgcgt tcgagcacga
     2881 actcgcgcgc ggtttcatcg tcggcgttga cttctcgcac gtccacaccg accgcataca
     2941 gcgcaaccgc gacctcaacc tgcccgcgcc gctctcagcc gaggagtaca tcaacttcct
     3001 gcgcgccaac aacacggcgg cgaacttcgc cgcgatggag gccagcggca ccatcgccac
     3061 catccgccag tcgggccgct cctacatcgc cgtcaacacg cccgccgcgc tgaccttccc
     3121 cgccaactcg gtcacgaccc gcctgcgccc gacgcaagca cagggcttgc tccagctcgg
     3181 cgccgtccag gtccgcgaat cgacggcggt gtccgactac gaccagttga ccttccgcac
     3241 ccgtttgatt cgcaggcgcg ggcaggtcaa cgtctactac acgctgtcgc gcagcctcgg
     3301 cgacgacgac aacgagcgtg acgcgggcgg cgtcgcctac gccgacgcgt acgacctgtc
     3361 gcgggagtac aacttctccc gcctcgaccg ccgccatcag ttcacggcga acccgattct
     3421 cttcctcccc tgggacttcg agatttcgag cgccatccgc ctccgctccg gcacgccgct
     3481 caatacgacc gtgggcgcgg acctgaacgg cgacacggtc aacaacgacc gcccgctcga
     3541 aagtccgggg gtggtcttca agcgaaacgc cttccgcaac cgcgccatct acgacatcga
     3601 cctgcgcgtg cagaagagcg tcttgttcgg cgagactcgt cggttgagct tctcggcgga
     3661 gttcttcaac ctcttcaacc gctcgaacat catcttcccg acgccgaata cggcgacctc
     3721 gtcgggaatc ctcgggcagt tctgcacgcc tacgagccag ctctgcggcc tgagcggttc
     3781 cacgaacccg ctcttcttgc agattaccga cccgaacacc ggcgcgctgc tggtcaacaa
     3841 caccaacccc ggcagtcagg tcttccagat gcagctcggc gcacgcttcc agttctaacc
     3901 gcaagcgccg actgctcgtg accgcttgaa acagggggcg cgcttcttct cgcaggaagc
     3961 gcgccccctc tcctggtgtt aggatgggtg tcgcgaccga ccgtttccac ctctatgagt
     4021 gagaggcgca gaaaagtccc ccgcgtgccc accgcgatcc ccgtcgagtg gggcgtgacg
     4081 agcgcttacg cgcacggcgg cagggtggtc agcctgagcg tgcgcggctg cctcatccgc
     4141 accgcgcacg tcgagccgct ctacggcaag accgtccacc tccgcttccc gctcccggac
     4201 gacgacctcc tcgcgctcgc cggccacgtc atctactacc tgcgcgacgt gggcttcgcc
     4261 gtcgagttcg acgcgccgcc cgacagggag cactacctgc tcgcgcgcct cgtgcaggac
     4321 taccgcaagg gcgaccccta caaagccccc ggcgccgacg cgctccaact cgtcgcgcgg
     4381 caagacgccc ccgccgtcac cttcaaagac cagcgccgac acgcgcgcgc caaggtctcc
     4441 atcaacgtcg attggggcga gacgccggcc tgcgagtacg gcggcgaccg cgtcaccagc
     4501 atcagcctcg gcggctgctt catccagacc gagcggcacg tcagggaagg cgccgtcgcc
     4561 tacgtccgcc tctggcagag gcccggtggc cgcggcgtct tccgcggcgt cgtccgctac
     4621 cagctccagc tcagcccccg gcacgcgccc atcggcctcg gcctcgaatt ccccgacctc
     4681 gacgacgaaa agacggccag cctcaaagaa gtcctcaact tctacggcga agccctctcc
     4741 acctgaaccg tcgaagcgcc ggacttcaca cgcgcacgcg cccgccgcct tgtcctaaga
     4801 caaggcgcga cctgtacgaa aacgctcgcg acacgacccc tctcaaacgc gcacgcgcgc
     4861 acgccccgcc gcctatcacg cgccccctca aacactttcg ccgccgcccg cgccgatggc
     4921 atgcacgttg ccgttgaagc ccaacgagaa aagtgattgc gggcgatctc gctcggcgtt
     4981 ctaacgtaac cttgagggag gaatttcgaa tcatgggcat accgaacaag gacgaggtgg
     5041 aaggcaaatt cgatcaggct aagggggccg tgaaagagaa cgtgggccgc gccgtcggcg
     5101 accgcgagat ggagaccgag ggcgccgccg accgcgccag cggcaacgtg caggaaggct
     5161 tcggcaccgc caagcgcaag gtcggcgacg ccgtcaagga catcggcgac gccatccgcg
     5221 actgaccttg actgcccctt caaccacgcg acctctcaaa cacgaaggcc gccgttcaac
     5281 ctcttcagag ttgaacggcg gccttcttac tttctctctc actccgacca tcgcaacgcg
     5341 attcgctaac cgacgctgct caccacggac tcttcactga ccaccgacca ccggccctta
     5401 ttcctgtctc acatactccg ccccgaggtc tgtcagcagc ttctccaacg cctccgcttc
     5461 ctcctccgtc ttcgcctcga cgccgatcag gatgccgccc gcgcgcaacg acgcgtcgta
     5521 gaccttcgcg cggtgctccg ggatgcccgc gccgaccagc gcgccgacca acccgcccgc
     5581 cgcgccgccc gcgcccgcgc ccgccagcgc cgccaccagc ggccccgcga cgatcagccc
     5641 cagacccggc aacgcgatgt tcgtcccgac cgccgccagc gccgcgatca ccgcgcccac
     5701 cgcgccgccc accgcgccgc cgatccccgc gccctcgccc gcgcgcgacc ccgtctcgat
     5761 ggcgaactcc ttactcttcg tcgcgtccga catcagcacg ctgatgtcgt cgcgcgtgta
     5821 gccgcgcttc aggatcgcgt ccaccgcggc ctccgcggcg acgcgcgtct tgaataaccc
     5881 tgtcacaagt ttattgactg ccataaaact tctcctcccg tccatgcgcc tcacgatacc
     5941 tctcatgaag acacgtcgcg gcactcgacc acaccggtcg ccgcccgggc gcgcctcaac
     6001 ataactcgcg ggggatgctt caatgttagt gcaacccgcg gagcgtgtcc attcgacagc
     6061 gggcagcagg cagcaggcag caggcagcgc aaggaagttc cgattgaggt ttgaaatctc
     6121 aggtcttaaa tcgtaaccac tgagttcacg attttctgtt ttctgactgc ctgctgcctg
     6181 cggcctgctg cccgctgggt tatactgtcc gccatgcgaa aacattcggg cgggggttcg
     6241 ggctgatgat cgaggccgtg attttcgacg ttgacgggac gctcgtcgat tcggttgacc
     6301 tgcacgcgcg cgcgtggcag gaggctttcg cgcgcttcgg caagcaggtt ccctacgaag
     6361 acgtgcgcta ccagatcggc aagggcggcg accaactgat gcccgtcttc ttctcgcgcg
     6421 aagagctgga cgacttcggc gaagagatgg agaagctgcg cggcgaacac ttcaagcgcg
     6481 agtacctgcc gcgcgtccgc gccttccccc gcgtgcgcga gttgttcgag cgcgtccgcg
     6541 ccgacggcaa gcgcatcgcc ctcgcctcgt ccgcgaagga agaagaactc gacgagtata
     6601 agaagatcgc ccgcatcgta gacctcgtcg aagaagagac ctcggcggac gacgccgaca
     6661 agtcgaagcc gcaccccgac atcttcgagg ccgcgctcgc ccagctcggg tctatcagcc
     6721 ccgaccgcgt gatcgtcgtc ggcgacacgc cctacgacgc cgaggctgcg cgcaaagcag
     6781 gcatacgcac catcggcctg ctctccggcg gcttccccga agaggatttg cgcaccgcgg
     6841 gctgcctcca aatcttcagg gacgccgccg acctcctcgc gcgctacgag gactcgctca
     6901 tcgcacgcga cccttcatca acggcgaccg cgcacgattg aagcgccggc gccccacgcg
     6961 gcacgcccga acgcccgtgc gcccgaacga acgcaatttg aaaatcctcc acgtcagctc
     7021 cgcgcgcacg ctcggcggcg gcgagcgtca tctcgcagac ctcgcgcgcg gcctcgcgcg
     7081 ccgcggccac gaagtctacg ccgcgctgcg cacggactcc ccgctccgcg cggagctact
     7141 ctcgtctttg ccccagcaga acatcttcac gctgccgctc aagggcgcgc tcgacctccc
     7201 gagcgcactc gaactcgcgc gactggcgcg cgagcacgag atcgacatcc tgcacgcgca
     7261 cctcgcgcgc gactacccgc ccgccgcgct cgccgcacgc cgcgcgcacg ccgcgcgact
     7321 cgtcatcacg cgccacgtcc tgttcccgat gaatcgcgcc cagcggctcg cgctctcgaa
     7381 cgccgcgcgc gtcatcgccg tctcgggcgg cgtcgcgcgc gcgctccgcg cgcgccacat
     7441 cttttccgaa cataaaatcc gcgtcgtccc caacgccatc gacgtcgcgc gcgtcgaggc
     7501 cgacctcggc ggcttcgagc gcgactctga tcgcgccgag actctgcgcg cgc
//
LOCUS       DBNGOH010000510         2735 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4656836, whole genome shotgun sequence.
ACCESSION   DBNGOH010000510 DBNGOH010000000
VERSION     DBNGOH010000510.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2735)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2735)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2735
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4656836"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..517
                     /locus_tag="AC159P_10015"
     CDS             <1..517
                     /locus_tag="AC159P_10015"
                     /inference="COORDINATES: protein motif:HMM:NF013126.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HIR3695828.1"
                     /translation="LAPYIELFQREDNMVHAVMVSHGGFPNIDIHRGIAGGRLVPASI
                     NPQIVTSLLRDELGFGGLVVTDDLEMGAIAKHCEIEEAALRAVLAGEDMLLICARTDL
                     IRRGYDALLGAAREGTLTHERIAASLRRIAAFKALTQPPHPFDEKRFAELSMQVAELN
                     TKLNYVYGGRI"
     gene            517..2121
                     /locus_tag="AC159P_10020"
     CDS             517..2121
                     /locus_tag="AC159P_10020"
                     /inference="COORDINATES: protein motif:HMM:NF012706.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR3695829.1"
                     /translation="MRTQGAVAVALTFVLAAALLAACSGGGGADPNAPFVTMLDSDPV
                     TLDPLDGTDAASERLRQLMFNSLVRKNEKFDYVGDLATDIDIAPDGLSATFTFPDNVT
                     FHDGKPLTSADARYTLESLFASNKKKKAPFFEPGADGGAAQAYITGIETPDARTLVLR
                     LRKPWNQLMPNLVSLPVIPQGSADTQKTEPVGSGPFQLVRRDESQKVVDLKAFEKYWQ
                     GASAIKNLRVRTILDANTLQTELESGGLDLVAGAANVSPDTFKYFSTHREFKVEQFPG
                     ANIVYLGFNVEQPPLDNVKVRQAISHAIDREAIVRDLMLGQARVAHSILPKDSWAYEA
                     GQVYDYDPAKAKRLLDEAGFRDSDGDGPGARFSRPVGLKISAGNAAVSQYAGVIQNAL
                     KAVGVPVEIETFETPTLIDQLSRGQYQMTTLRWVGGNQDPIFLYDLFHSGEIPSATRP
                     NGRNRQRYRNPELDRLLAEAINTIDRQKARTLYVQAQQIVSRDVPMLTLWYPDVLVIA
                     RKSVGNIKVDASNDFSFLRSVTVGNQ"
     gene            complement(2267..>2735)
                     /locus_tag="AC159P_10025"
     CDS             complement(2267..>2735)
                     /locus_tag="AC159P_10025"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:NF013468.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HIR3695830.1"
                     /translation="REGKPILVLVAERADAPRLWETAPPLFDALAARHWPGALTVVCA
                     ARRGLPEELTAGTGTVGVRLPADEEVRALVRACGGALTATSANPAGEPPARSAADVER
                     YFQHRIDLVIDGGETKVERPSTVLDIHGGTPVLVREGVVTRAEIEETLKISRR"
BASE COUNT          473 a         1040 c          823 g          399 t
ORIGIN      
        1 cctcgcgccc tacatcgagc tgttccagcg cgaggacaac atggtgcacg ccgtgatggt
       61 cagccacggc ggcttcccca acatcgacat ccaccgcggc atcgcgggcg ggcggctcgt
      121 cccggcctcg atcaacccgc agatcgtgac cagcctgctg cgcgacgagc tgggcttcgg
      181 cggcctcgtc gtcacggacg acctcgagat gggcgccatc gccaagcact gcgagatcga
      241 agaggccgcg ctgcgcgccg tcctcgcggg cgaagacatg ctcctcatct gcgcgcgcac
      301 ggacttgatc cgccgcggct acgacgcgct gctcggcgcc gcgcgcgaag gcactctgac
      361 gcacgagcgc atcgcggcct cgctccgccg catcgccgcc ttcaaagccc tgacgcagcc
      421 gccccacccc ttcgacgaga aacgcttcgc agagctgtcg atgcaggtgg ccgaactcaa
      481 caccaagctg aactacgtct acggagggag aatctgatgc gtacacaggg ggccgtcgcc
      541 gtcgccttga ctttcgtgct cgccgcggcg ctcctcgccg cctgctccgg cggcggcggc
      601 gccgacccga acgcgccgtt cgtgaccatg ctcgacagcg accccgtcac actcgacccg
      661 ctcgacggca ccgacgcggc cagcgagcgc ctccgccagc tcatgttcaa ctcgctcgtg
      721 cgcaagaacg agaagttcga ctacgtcggc gacctcgcca ccgacatcga catcgcgccc
      781 gacggcctct ccgcgacctt caccttcccc gacaacgtga ccttccacga cggcaagccc
      841 ctgacctccg cggacgccag gtacacgctc gaaagcctct tcgcctcgaa caagaaaaag
      901 aaggcgccct tcttcgaacc cggcgcggac ggcggcgccg cgcaagctta catcacaggc
      961 atcgagacgc ccgacgcgcg cacgctcgtc ctgcgcctgc gcaagccctg gaaccagctc
     1021 atgccgaacc tcgtctcgct gcccgtcatc ccgcagggca gcgccgacac gcagaagacg
     1081 gagccggtcg gctccggccc cttccagctc gtccgccgcg acgagtcgca gaaggtcgtc
     1141 gatctcaagg cgttcgagaa gtactggcag ggcgcgtcgg ccatcaagaa cctgcgcgtg
     1201 cgcaccatcc tcgacgccaa caccttgcag accgagctgg agagcggcgg cctcgacctc
     1261 gtcgcgggcg cggccaacgt ctcgcccgac accttcaagt acttctccac gcacagggag
     1321 ttcaaggtcg agcagttccc cggcgcgaac atcgtctacc tcggcttcaa cgtcgagcag
     1381 ccgccgctcg acaacgtcaa ggtgcgccag gccatctccc acgccatcga ccgcgaggcc
     1441 atcgtccgcg acctcatgct cggccaggcg cgcgtcgcgc actccatcct gccgaaagac
     1501 tcctgggcct acgaggccgg gcaggtctac gactacgacc ccgcgaaggc aaaacgactg
     1561 ctcgacgagg cgggcttccg cgactccgac ggcgacggcc ccggcgcgcg cttttccaga
     1621 cccgtcggcc tcaagatttc ggcgggcaac gccgccgtca gccagtacgc gggcgtcatc
     1681 cagaacgcgc tcaaggccgt cggcgtgccc gtcgagatcg agaccttcga gacgccgacg
     1741 ctcatcgacc agctcagccg cggccagtac cagatgacca cgctccgctg ggtcggcggc
     1801 aaccaggacc cgatcttcct ctacgacctc ttccactccg gcgagattcc cagcgccacg
     1861 cgccccaacg gtcgcaaccg ccaacgctac cgcaaccccg aactcgaccg cctcctcgcc
     1921 gaggccatca acaccatcga ccgccagaag gcgcgcaccc tctacgtgca ggcccagcag
     1981 atcgtcagcc gcgacgtgcc catgctcacc ctctggtatc cggacgtgtt ggtcatcgcg
     2041 cgcaaaagcg tcggcaacat caaagtggac gccagcaacg acttcagctt cctccgcagc
     2101 gtcacggtcg ggaatcagta ggcggtaggc agcaggcagt caagacagaa gggcggagag
     2161 gcttgggcgg tcacgtcctt agcctctctg cccttcgatc ataaaagcaa gaggcttgag
     2221 acgttacctc tcaagcctct tgcttttact gcctactgcc taccgcctac cgcctactga
     2281 tcttcaacgt ctcttctatc tcggcgcgtg tgacgacgcc ttcgcggacg aggacgggcg
     2341 tgccgccgtg tatgtcgagg acggtcgagg gccgctcgac tttcgtctcg ccgccgtcga
     2401 tgacgaggtc gatgcgatgt tggaagtagc gctccacgtc cgcggcactg cgcgcgggcg
     2461 gctcgcccgc ggggttggcg ctggtcgccg tcagcgcgcc gccgcaggcg cgcacgagcg
     2521 cgcgcacctc ttcgtccgcg ggcagccgca cgccgaccgt gcccgtgccc gccgtcaact
     2581 cctccggcag gccgcgccgc gcggcgcaga cgacggtgag cgcgccgggc cagtgcctcg
     2641 cggccagcgc gtcgaagagc ggcggggccg tctcccacag gcgcggggcg tccgcgcgct
     2701 ctgcgacgag gacgaggatg ggcttgcctt ccctg
//
LOCUS       DBNGOH010000511         2875 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2091516, whole genome shotgun sequence.
ACCESSION   DBNGOH010000511 DBNGOH010000000
VERSION     DBNGOH010000511.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2875)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2875)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2875
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2091516"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2875)
                     /locus_tag="AC159P_10030"
     CDS             complement(<1..>2875)
                     /locus_tag="AC159P_10030"
                     /inference="COORDINATES: protein motif:HMM:NF051698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HIR3695831.1"
                     /translation="CCLLLALALWPAAAAAQTIVGRVSGTVTDASGAVVPGASVTVTN
                     AATNLVRTATTDADGFYTVTNLPVGTYVIAVESQGFKRAEQRGVALTADARLTIDLAL
                     ETGQVTETVQVSTALGETVNNTSGEVARVIDSQQVQNLALNGRNYIQLLSLIPGVAQT
                     TDDQLELSTSLATNQQSINGNRGQMTNMTVDGGTNLQSGSNASQINNVGIDFIQEVKV
                     QTSNFSAEYGRNSGAQINVVTRSGGNEFHGSAFEFLRNDKLDARNFFAPVRPALRYND
                     YGYSFGGPIIKDKFFFFGGQEWKKIRRLANPVVRTVPTLAELNGDFSFRLRGADGVVG
                     TNDDGLLRDPNSTQPCTTANRAGCFGGTNVALRNIIPANRITPDGAAIASVYRTMIGL
                     STSFTNLPVGNNVTFQQPNPFNFREDIIRLDYRFNSNHSLYGRYIHDNNDLIDPFGTF
                     INSQLPTVPSNRLRPGNSIQVAYTWLITPKLINEAKVNTSWVSQHIPPAGEFWQRDTY
                     GFQYQQLFAGGGVYADSIPDTTVNGFASFAGAARSLVSPTTDIMIGDNITWVRGNHTI
                     KSGGLVYRNRIDQNGRTTYAGNLNFNTGGNTRTSNNSFADALLGNFRTYSEASLDPVG
                     FFRFTQFEAFVTDSWKVNRKLSLELGVRYQYGTPIYTQANNLTNFDPSLYDPAQAVTV
                     LANGTIDPARGGNRFNGLIRAGSGVPAEELGRVPGGDSAAVQAVPTGAPRGLYEGHHY
                     FMPRVSFAYAPFDDNKTAIRGGFGMYYDRIEGNLIFPSIGNPPFTGSAQFSNANLSNI
                     TSGSTTVAPFADIFAIDPNLDTPYTMSYSLSVQRELPLGVFAEAAYVANLGRHLIRQP
                     DINQATFDALRANAALPTAQRVDTNALRPFKGYSAIRMRLSDATSNYHGMQLFAAKRR
                     GDLLMTLSYTWSKVLTDASGFNDNPEDPFNRDFNYGPATFDRRH"
BASE COUNT          425 a          845 c         1067 g          538 t
ORIGIN      
        1 gtggcggcgg tcgaaggtgg ccgggccgta gttgaagtcg cggttgaagg ggtcttccgg
       61 gttgtcgttg aagccgctcg cgtcggtcag gactttcgac caagtgtagc tgagcgtcat
      121 caacaaatcg ccccggcgct tggccgcgaa gagctgcatc ccgtggtagt tcgaggtcgc
      181 gtccgaaaga cgcatgcgga tcgccgagta gcccttgaag ggccggagcg cgttcgtgtc
      241 cacgcgctgc gccgtcggca gcgcggcgtt cgcgcggagc gcgtcgaagg tcgcctgatt
      301 gatgtcgggc tggcggatca ggtggcgacc gaggttggcg acgtaggccg cctcggcgaa
      361 gacgcccagc ggcagctcgc gctgcacgct caggctgtag ctcatcgtgt agggcgtgtc
      421 gaggttcggg tcgatggcga agatgtctgc gaagggcgcg acggtggtcg agccgctggt
      481 gatgttcgag aggttcgcgt tgctgaactg cgcgctcccc gtgaagggcg gattgccgat
      541 ggaggggaag atgaggttgc cttcgatgcg gtcgtagtac atcccgaagc cgccccggat
      601 cgccgtcttg ttgtcgtcga acggcgcgta ggcgaaactc acgcgcggca tgaaatagtg
      661 atggccttcg tagagaccgc gcggcgcgcc cgtgggcacg gcctgcacgg cggcgctgtc
      721 gccgcccggc acgcgcccca gctcttccgc ggggacgccg ctgccggcgc ggatcaggcc
      781 gttgaagcgg ttgccgccgc gcgccgggtc gatcgtcccg ttcgccagca cggtgacggc
      841 ctgcgccggg tcgtacagcg acgggtcgaa gttggtcagg ttgttggcct gcgtgtagat
      901 gggcgtcccg tactggtagc gcacgccgag ttcgaggctg agcttgcggt tgaccttcca
      961 gctatcggtg acgaacgcct cgaactgcgt gaagcggaag aagccgacgg ggtcgagcga
     1021 cgcctcgctg taggtgcgga agttgcccag cagcgcgtcg gcgaaagagt tgttcgaggt
     1081 gcgtgtgttg ccgcccgtgt tgaagttgag gttgccggcg taggtcgtgc gcccgttctg
     1141 gtcgatgcgg ttgcgataga cgaggccgcc gctcttgatc gtgtgattgc cgcgcaccca
     1201 cgttatgttg tcgccgatca tgatgtcggt cgtcggcgag acgagcgagc gggccgcgcc
     1261 ggcgaagctg gcgaagccgt tgacggtcgt gtccgggatg ctgtcggcgt agacgccgcc
     1321 gcccgcgaag agctgctggt actggaagcc gtaggtgtcg cgctgccaga actccccggc
     1381 gggcgggatg tgctgcgaga cccaggaggt gttgaccttc gcctcgttga tgagcttcgg
     1441 cgtgatgagc cacgtgtacg cgacctgtat cgagttgccg ggccgcagac ggttgctcgg
     1501 caccgtgggg agctgcgagt tgatgaaggt gccgaacggg tcgatgaggt cgttgttgtc
     1561 gtggatgtag cgaccgtaga ggctgtggtt cgagttgaaa cggtagtcga gccggatgat
     1621 gtcctcgcgg aagttgaacg ggttcggctg ctggaaggtg acgttgttgc cgacgggcag
     1681 gttcgtgaag ctggtcgaga ggccgatcat cgtgcggtag acgctggcga tggccgcgcc
     1741 gtccggcgtg atgcggttgg cggggatgat gttcctgagc gccacgttcg tgccgccgaa
     1801 acagcccgcg cggttcgccg tcgtgcaggg ctgcgtgctg ttcgggtcgc ggagcagacc
     1861 gtcatcattg gtgcccacga cgccgtcggc cccgcgcagg cggaacgaga agtcgccgtt
     1921 cagctcggcc agcgtcggca ccgtgcggac gaccgggttg gcgaggcggc gaatcttctt
     1981 ccactcctgc ccgccgaaga agaagaactt gtccttgatg atggggccgc cgaagctgta
     2041 gccgtagtcg ttatagcgca gcgcgggccg cacgggcgcg aagaagttgc gcgcgtccag
     2101 cttgtcgttg cgcaggaact cgaacgccga gccgtgaaac tcgttgccgc cgctccgggt
     2161 cacgacgttg atctgcgcgc ccgagttgcg cccgtactcg gccgagaagt tcgaggtctg
     2221 gactttgacc tcctggatga agtcgatgcc gacgttgttg atctggctgg cgttgctgcc
     2281 ggactgcaag ttcgtcccgc cgtccacggt catgttcgtc atctgcccgc ggttgccgtt
     2341 gatcgactgc tggttggtgg cgaggctcgt cgaaagctcc aactggtcgt cggtcgtctg
     2401 cgcgacgccg gggatgagcg agagcaactg gatgtagttg cgcccgttca aagcgaggtt
     2461 ctgcacctgc tggctgtcga tgacgcgcgc cacctcgccc gaggtgttgt tgaccgtctc
     2521 gcccaaagcg gtcgagacct gcaccgtctc cgtcacctgc ccggtctcca gcgcgaggtc
     2581 gatggtcagg cgcgcatcgg ccgtcagggc cacgccgcgc tgctcggcgc gcttgaaacc
     2641 ctggctctcg acggcgatga cgtacgtccc gacgggcagg ttcgtcaccg tgtagaagcc
     2701 gtcggcgtcc gtggtcgccg tgcgcacgag gttcgtcgcg gcgttcgtca cggtgacgga
     2761 cgcgccgggc acgaccgcac ccgaggcgtc cgtcaccgtg cccgagacgc ggccgacaat
     2821 cgtctgcgcc gcagccgccg cgggccagag cgcgagggcg agcagcagac agcag
//
LOCUS       DBNGOH010000512         2228 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3558253, whole genome shotgun sequence.
ACCESSION   DBNGOH010000512 DBNGOH010000000
VERSION     DBNGOH010000512.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2228)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2228)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2228
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3558253"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(142..954)
                     /locus_tag="AC159P_10035"
     CDS             complement(142..954)
                     /locus_tag="AC159P_10035"
                     /inference="COORDINATES: protein motif:HMM:NF024743.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HIR3695832.1"
                     /translation="MKATLRSPHTREARPPHTRASVAAPARAGVVALALLLVCLAAGA
                     EGRAQRKSFDKEYPAQQPRVVVQLYNRLGTVEVETWEKNKIKVTAKMESSKASVIPEM
                     SGNSIRINVPRDNRDDVGDINFKIMVPADSTVDISTMVGNIIIRNVRGETVRAKVSSE
                     GDIELTGIRAQTVVAENTRGNILFDAELLRDGVYDLSSTRGDIQLRITASSGFRLMAT
                     SLKTRSINLGGFAGKGDFNFTNNNRQVVGKVGDGGAVLSTTNGLGSIVFMQR"
     gene            complement(1072..1800)
                     /locus_tag="AC159P_10040"
     CDS             complement(1072..1800)
                     /locus_tag="AC159P_10040"
                     /inference="COORDINATES: protein motif:HMM:NF024881.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HIR3695833.1"
                     /translation="MNCEKCQELLSDYLDGVLGHRERAALGAHLGACPACAEVREELR
                     SIVSAARATREQLSAPPDERALWLRIREAVEHEEGFVPVAASAAAAPARAREGFWTRL
                     LDRRWEFSLPQLASGVAALAVAVAFVTTLGVQYVSNERSTGGGRPRPAARDTALTGFR
                     PQTYLQPHQANLQYWQQRVEQRKATWNPRMRESFDRSVSVLDEAMTESMGELERNPHD
                     EVAEQMLNAALRDKIELLREFGEQ"
     gene            complement(2008..>2228)
                     /locus_tag="AC159P_10045"
     CDS             complement(2008..>2228)
                     /locus_tag="AC159P_10045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014263877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR3695834.1"
                     /translation="DAMPVVDRIALDIAVSQLPPGYRAVFVLHDVEGHEHEEIARLLG
                     CSVGTSKSQLHKARMKLRTLLRQQNPPR"
BASE COUNT          300 a          722 c          802 g          404 t
ORIGIN      
        1 ggcggcgttc ttcttcgacg cttcctgcct gcctttccgg cgcgcgccga ccgacgccga
       61 cggggcgttg atgaaggcga cggtgctgag caggagtgcg gagacgaaaa gcgcggaggt
      121 ggagttgttc atcgggcgcg ctcagcgctg catgaagacg atggagccga ggccgttggt
      181 cgtcgagagc acggcgcccc cgtcgccgac cttgccgacg acctgacggt tgttgttggt
      241 gaagttgaag tcgcccttgc cggcgaagcc gccgaggttg atgctgcgcg tcttgaggct
      301 ggtcgccatc aggcggaagc cggacgaggc cgtgatgcgg agctggatgt cgccgcgcgt
      361 ggagcttaga tcgtaaacgc cgtcgcgcag cagctcggcg tcgaagagga tgttgccgcg
      421 cgtgttctcg gcgaccacgg tctgcgcgcg gatgccggtc agctcgatgt cgccctcgct
      481 ggagactttg gcgcggacgg tctcgccgcg cacgttgcgg atgatgatgt tgccgaccat
      541 cgtcgagatg tccacggtcg agtccgccgg caccatgatc ttgaagttga tgtcgcccac
      601 gtcgtcgcgg ttgtcgcgcg ggacgttgat gcggatgctg ttgccgctca tctcggggat
      661 cacgctcgcc ttggacgact ccatcttggc cgtcaccttg atcttgttct tctcccacgt
      721 ctcgacctcg acggtgccga ggcggttgta gagctggacg acgacgcgcg gctgctgcgc
      781 cgggtactcc ttgtcgaagc tcttgcgctg cgcgcggcct tcggcgcccg cggcgaggca
      841 gaccaggagg agggcgaggg cgaccacgcc ggcgcgcgcc ggggcggcca cgctcgctct
      901 cgtgtgcggc gggcgcgctt cgcgcgtgtg agggcttcgg agtgtcgctt tcatcaacct
      961 tgctgtctcg gatgtcccgg ggtttctcgg tctcgccgaa gcgcccgcgc tccgtctgag
     1021 agagaggcgc gggcgcgagt attgtaaccc gttcgtgccg gtgtgcaacc gctactgctc
     1081 gccgaactcg cggagcagtt cgatcttgtc gcgcagcgcg gcgttgagca tctgctcggc
     1141 gacctcgtcg tgcgggttgc gctcaagctc gcccatcgat tcggtcatcg cctcgtcgag
     1201 cacggagaca ctgcggtcga acgactcgcg catgcgcggg ttccaggtgg ccttgcgctg
     1261 ctcgacgcgc tgctgccagt actgtaggtt cgcctgatgc ggctgaagat aggtctgcgg
     1321 gcggaagccc gtcagcgccg tgtcgcgcgc cgcgggacgt ggcctgccgc cccccgtgct
     1381 gcgttcgttc gagacgtact gcacgccgag cgtggtcacg aaggcgaccg cgacggcgag
     1441 cgccgccacg cccgaagcga gctgcggcag cgaaaactcc caacgcctgt cgagcaggcg
     1501 cgtccagaac ccttcgcgcg cgcgcgcggg cgcggcggcg gctgaagcgg cgaccgggac
     1561 gaagccctct tcgtgctcga cggcctcgcg gatgcggagc cagagggcgc gctcgtcggg
     1621 aggcgcgctc agttgctcgc gcgtggcgcg ggcggcggag acgatggagc ggagttcttc
     1681 gcgcacctcc gcgcacgcgg ggcatgcgcc gaggtgcgcg ccgagcgcgg cgcgctcgcg
     1741 gtggccgagc acgccgtcaa ggtagtcgct gagtaattcc tgacacttct cgcagttcat
     1801 ttccctaacc tctcgcgccc tgcggctcta cggcttgcct gacgccgctc gcgggcggcg
     1861 atggggagaa gcgcctccct ttcggcgggc ggcactttgg cgggcgctac ttttcaaacc
     1921 atgcgggccg gtctactggg gagacgcggt gtcttgaatt ttggcggctc gtcggtcggg
     1981 cgacttgcgg cccacacgcg ttggaagcta ccgcggcgga ttctgttgcc tcaacagcgt
     2041 gcgcagcttc atgcgggcct tgtggagctg cgacttcgag gtccccaccg agcagcccaa
     2101 gagccgcgcg atctcctcgt gctcgtgccc ctccacgtcg tgcaacacga acaccgcccg
     2161 gtagcccggc gggagctgcg agacggcgat gtcgagcgcg atgcgatcca cgacgggcat
     2221 cgcgtccg
//
LOCUS       DBNGOH010000513         1576 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1726426, whole genome shotgun sequence.
ACCESSION   DBNGOH010000513 DBNGOH010000000
VERSION     DBNGOH010000513.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1576)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1576)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1576
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1726426"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            88..1113
                     /locus_tag="AC159P_10050"
     CDS             88..1113
                     /locus_tag="AC159P_10050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA0069 family radical SAM protein"
                     /protein_id="HIR3695835.1"
                     /translation="MRYIDNPPNPWLDTTVEWIGEPPESRVEVFEETATRSIITHNKS
                     PDVGFDYSVNCYRGCTHACSYCFSRPTHEYLGYGAGTDFETKIVAKVRAPELLRAEFM
                     RPGWRGDVLMFSFTSDPYLPLEAHYKLTRRCLEVCLEFRNPVGLVTKSALVRRDVDLL
                     AALAREAEASVFFTIPFSDYETARALEPFAPSPDARFRAMAELAAAGIPVGIGVAPVI
                     PGLNDPHIPALLKRAKEAGAAEAFMTMLRLPGSVAPYFEQRLRERLPTKAERVLARVR
                     EARGGRLNNSEFGERMRGKGEYWQVVERSFRLHCARLGLNNEDGRTYADHKPSTFRRP
                     SAQGSLF"
     gene            1343..>1576
                     /locus_tag="AC159P_10055"
     CDS             1343..>1576
                     /locus_tag="AC159P_10055"
                     /inference="COORDINATES: protein motif:HMM:NF012780.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIR3695836.1"
                     /translation="MCNRESYFVRDTATAFDAADYMAERNIGAVCVLDAEDKLCGIFS
                     ERDLLKRVIVAHLDPADVPIRQVMSEPRAVIDCS"
BASE COUNT          275 a          562 c          474 g          265 t
ORIGIN      
        1 cctccgcact tctatttttg ctgcccgctg cccgctgccc gctgcccgct gtttgctgct
       61 ataatgcccc cactcccgat gtaactcatg cgctacatcg acaacccgcc caacccgtgg
      121 ctcgacacga cggtcgagtg gatcggcgag ccgccggaat cccgcgtcga ggtgttcgag
      181 gagaccgcga cgcgctccat catcacgcac aacaagagtc cggacgtggg cttcgactac
      241 tccgtcaact gctaccgcgg ctgcacgcac gcctgtagct actgcttcag ccgaccgacg
      301 cacgaatacc tcggctacgg cgcgggcacc gacttcgaga cgaagatcgt ggcgaaggtg
      361 cgcgcgcccg aactcttgcg cgccgagttc atgcggcccg gctggcgcgg cgacgtgctc
      421 atgttctcgt tcacgtccga cccctacctg ccgctcgaag cgcactacaa gctgacgcgc
      481 cgctgtctcg aagtctgcct cgaattccgc aacccggtcg ggctggtcac gaagtccgcg
      541 ctcgtgcggc gcgacgttga cctcctggcc gcgctcgcgc gcgaggcaga ggccagcgtc
      601 ttcttcacca tccctttctc cgactacgag acggcgcgcg ctctggagcc gttcgcgccc
      661 tcgcccgacg cgcgcttccg cgcgatggcc gaactcgcgg cggctgggat accggtcggc
      721 atcggcgtcg cgcccgtcat ccccggcctc aacgacccgc acatccccgc gctcctcaag
      781 cgcgcgaagg aggcgggcgc cgccgaagcc ttcatgacga tgctgcgcct gcccggcagc
      841 gtcgcgccct acttcgagca gcgcctgcgc gaacgcctgc cgacgaaggc cgagcgcgtg
      901 ctcgcgcgcg tccgcgaagc gcgcggcggt cgcctcaaca acagcgagtt cggcgagcgc
      961 atgcgcggca agggcgaata ctggcaggtc gtcgagcgca gcttccgcct gcactgcgcg
     1021 cgcctcggcc tcaacaacga ggacgggcgc acctacgccg accacaagcc gagcaccttc
     1081 cgccgcccct ccgcgcaggg aagtctcttt tgaagcagga gacagaagtc agaagacgga
     1141 agtcggaata agaaaagtgg tctccgcctt tcttctgatt tctgtctccc gacttctgtc
     1201 ttctgcttta tgagaaaaga aggacaagtg ccgccacgga aaactcttcg aatcgccgcg
     1261 cgcatgtctg tataatgtcc tggctcgaac catcacgcga gacgcggagg gatacaagcg
     1321 catgacgaca atcaggcatg tggtgtgtaa ccgtgaatcg tatttcgtgc gcgacacggc
     1381 gacggcgttc gacgccgccg attacatggc ggagcgcaac atcggggccg tctgcgtgct
     1441 cgacgccgaa gacaagctgt gcggcatctt ctccgagcgc gacctgctga agcgcgtcat
     1501 cgtcgcgcac ctcgacccgg cggacgtgcc catccggcag gtgatgagcg agccgcgcgc
     1561 cgtcatcgac tgctcc
//
LOCUS       DBNGOH010000514         3773 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1359928, whole genome shotgun sequence.
ACCESSION   DBNGOH010000514 DBNGOH010000000
VERSION     DBNGOH010000514.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3773)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3773)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3773
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1359928"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..324)
                     /locus_tag="AC159P_10060"
     CDS             complement(<1..324)
                     /locus_tag="AC159P_10060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695837.1"
                     /translation="MFSRLLLAAVALVAAASAFPALAQPPSTTTSLGDKNMKDEDTVR
                     ARSVEMERIRRDAAKPDRKPEPSPEDRFPQIKEDFERMQIVNGDVLQAPGAPDYARLA
                     EAAEEL"
     gene            619..3039
                     /locus_tag="AC159P_10065"
     CDS             619..3039
                     /locus_tag="AC159P_10065"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019399535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HIR3695838.1"
                     /translation="MNRRDFLYLPTVALLTRAAQGGPAAAAHAQTADAAPPQPRETTL
                     WYRRPAASWNEALPVGNGRLGAMVFGGVESERLQLNEDTVWAGERRDRVNPEGLRHLA
                     EVRRLLFAGKPKEAEELAERTIIATPKRMPPYQPLGDLLLRFEGQAEATDYVRELDID
                     TGIARISFQSKGARFTREVFSTAVDQVIVVRLSADRPGRISFAATLTREKDGTTRTID
                     RDRVVLEGEAIARGDRHAQERKVGVKFCGLLRVVPEGGRVRADGERVSVEGADAATLL
                     LAAATNFRERVPSSKCAAQLAAAAAKPFAQLRAAHVRDHRRLFRRVAFSLGAAAPELP
                     TDERLKRVQEGTADLALEALYFQFGRYLLIASSRPGTMAANLQGIWNEQLQPSWDSKY
                     TVNINTEMNYWPAEVTNLSELHEPLFDLIDNARPEGRRVARKLYGARGFVVHHNTDLW
                     GNAVPIDGVGSGIWPTGAAWLSLHLWDHYDYTRDRSFLARRAYPVLKEAAEFFLDYLV
                     EDGKGHLITGPSLSPENRYRAPDGAAVKLCMGPAMDTQIVHALFTRVIEAGRLLKTDA
                     EFVRRVAAARARLPPMKVGRHGQLQEWLEDYEEPDPGHRHISHLFALHPDDQITLRGT
                     PELARAARVSLERRLRAGSGHTGWSRAWIINFWARLEEGDAAHENVVALLAKSTLPNL
                     LDNHPPFQIDGNFGGTAGMAEMLLQSHGGVISFLPALPRAWPQGSVTGLRARGAVEVD
                     VSWDEGKATHATLRTRVGGEHRLRAPRGQRIASVMEGRRRIRFAADADGLVRLPLDAR
                     REYRVTFR"
     gene            3264..>3773
                     /locus_tag="AC159P_10070"
     CDS             3264..>3773
                     /locus_tag="AC159P_10070"
                     /inference="COORDINATES: protein motif:HMM:NF025174.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR3695839.1"
                     /translation="MKIALIIGAVLCALFTTPAALAAEKAVAPRWVRVAPGVWRAQVG
                     RPESLTLLGAAGAKPALEALRRMSDAAFPLDRGKIEARQWDWKTALRFPLALDEEVYG
                     LGVDFRTMRRTGSVFLLHVDHWGGQTGRTHAPVPLYVSTRGYAVLFNTARYLKVNVGH
                     GVRLAAEDKP"
BASE COUNT          621 a         1293 c         1269 g          590 t
ORIGIN      
        1 caactcctcc gccgcctccg ccagacgcgc gtagtcgggc gctcccgggg cctgcaacac
       61 gtcgccgttg acgatctgca tccgctcgaa gtcttccttg atctgcggga atctgtcttc
      121 gggactcggc tcgggcttgc ggtcgggctt ggcggcgtcg cgccggatgc gttccatctc
      181 gaccgaccgc gccctgaccg tgtcttcgtc cttcatgttc ttatccccga gactggtggt
      241 cgtgctgggc ggctgggcca gcgcggggaa agcggacgcg gcggcgacga gcgcgaccgc
      301 cgccagtaga agccgactga acatgacttg cacctccgcg ggcagtttaa acccgagcga
      361 ccgcagacgc gaaacaaaaa aaagcggcgt tgaattatcc gcccgccgcc ggcctcatcc
      421 cttccccgcg cggcgacgcg ctttgcgccg gtcgcgccgc ggaaaaaagg gttgcccgtg
      481 ttatcggtaa cagatattat cctcttcatc ttcattcggg cgtcaaggcc accgcgttac
      541 agtccactgt cgccgagcac tacgcaaact tgagaaggcg tcgggaagaa cttcgcgccc
      601 gcgccgcgcg agacgtgtat gaaccggaga gacttccttt acctgccgac cgtcgccctg
      661 ctgacacgag ccgcacaggg cggccccgcc gccgccgcgc acgcgcagac ggcggacgcg
      721 gccccgccgc agccgcgcga gacgacgctc tggtatcggc ggcccgccgc gagttggaac
      781 gaggcgctgc ccgtcggcaa cgggcggctg ggcgcgatgg tcttcggcgg cgtcgagtcc
      841 gagcggcttc agctcaacga ggacacggtc tgggccggcg agcggcgcga ccgcgtcaac
      901 cccgaaggct tgcgccacct cgccgaagtg cgtcgcctgc tcttcgctgg caagcccaaa
      961 gaggcagagg agttggccga gcggacgatc atcgccacgc ccaaacggat gccgccctac
     1021 cagccgctcg gcgacctgct gctccgcttc gaagggcagg cggaggcgac cgactacgtg
     1081 cgcgagttgg acatcgacac agggatcgcc cgcatctcct ttcaatcgaa gggcgcgcgc
     1141 ttcacccgcg aggtcttctc gaccgcggtc gatcaagtca tcgtcgtccg cctgagcgcc
     1201 gacaggccgg gccgcatctc tttcgcggcg acgctcacgc gcgagaagga cggcacgact
     1261 cgcaccatcg accgcgaccg cgtggtgctc gaaggcgaag cgattgcgcg cggcgaccgc
     1321 cacgcgcagg agcgcaaggt cggcgtcaag ttctgcggcc tgctgcgcgt cgtgcccgaa
     1381 ggcggtcgcg tccgcgccga cggcgagcgc gtctcggtcg aaggcgcgga cgccgccacg
     1441 ctgctcctcg ccgccgcgac aaatttccgc gaacgcgtgc cttcgtcgaa gtgcgccgca
     1501 caactcgccg ccgccgcggc gaagcctttc gcgcagcttc gcgccgcgca cgtccgagac
     1561 caccggcggc tgttccgccg cgtcgcgttc agcctcggcg cggcggcgcc ggaactgccg
     1621 accgacgagc gcctgaagcg cgtgcaggag ggcaccgcag acctcgcgct cgaagcgctg
     1681 tattttcagt tcggtcgtta tctgctcatc gccagctcgc ggcccggcac gatggcggcg
     1741 aaccttcagg gcatctggaa cgaacagcta cagccctctt gggacagcaa gtacacggtc
     1801 aacatcaaca ccgagatgaa ctactggccg gcggaggtga cgaacctctc ggagttgcac
     1861 gagcctctgt tcgacctcat cgacaacgcg cgccccgaag gccgccgcgt ggcgcgaaag
     1921 ctttatggcg cgcgcggctt cgtcgtccac cacaacacgg acttgtgggg caacgccgtg
     1981 ccgattgacg gagtcggctc cggaatctgg ccgacgggtg cggcgtggct gagcctgcac
     2041 ctgtgggatc actacgacta cacgcgcgac cgctcgttcc tcgcgcggcg ggcttacccg
     2101 gtgctcaagg aggccgccga gttcttcctc gattatctgg tcgaggacgg caagggtcac
     2161 ttgattacgg ggccgtcgct ctcgcccgag aatcgctacc gcgcgccgga cggcgccgcc
     2221 gtcaagctct gcatggggcc tgcgatggat acgcagatcg tccacgcgct cttcacgcgc
     2281 gtcatcgaag cgggccgcct gctcaagacg gatgccgaat tcgtgcgccg cgtcgccgcc
     2341 gcgcgcgcga ggttgccgcc gatgaaggtc ggcaggcacg ggcagttgca ggagtggctc
     2401 gaagactacg aagagccaga ccccggccac cggcacatct cgcacctctt cgcgctccac
     2461 ccggacgacc agatcaccct gcgcgggacg cctgagctgg cacgcgcggc gcgcgtctcg
     2521 ctcgaacggc ggctgcgcgc ggggagcggg cacacgggtt ggagccgcgc gtggatcatc
     2581 aacttctggg cgcggctcga agagggcgac gcggcacacg agaacgtggt cgcgctgctc
     2641 gccaaatcga cgctgccgaa cctgctcgac aaccacccgc ccttccagat cgacggcaac
     2701 ttcggcggca ccgcagggat ggccgagatg ctcttgcaga gccacggcgg cgtcatcagc
     2761 ttcctgcccg cgctcccgcg cgcgtggcct caagggtccg tcaccggcct ccgcgcgcgc
     2821 ggcgcggtcg aggtcgatgt cagttgggat gagggcaagg cgacgcacgc gactttgcgc
     2881 acgcgcgtcg ggggcgagca cagactgcgt gccccgcgcg gtcagcgaat cgcttccgtg
     2941 atggagggac gccgaagaat ccgtttcgcg gcggacgccg acgggctggt gcgtctgccg
     3001 ctcgacgcgc ggagagaata ccgcgtgacg ttccgctgag aagcgacccg cgcgttgcgt
     3061 cgccgagatg ccgggcaagt agaggacaat gatgcagaat aaccggaacg gttttcaaag
     3121 tgatgtgaga cgttcgttgt aactgtaggg gcagggcttg tccctgcccg taagattcgg
     3181 tcgattcaat cttgagcgtt agggacgctg cgcgggcaga gacaagccct gcccctacaa
     3241 cttcacccac gcgctatttt caaatgaaga ttgccttaat catcggcgca gtcctctgcg
     3301 cgttgttcac gactcccgcc gcgctcgccg ctgaaaaggc ggtcgcgccg aggtgggtgc
     3361 gcgtggcccc cggcgtgtgg agggcgcagg tcggcaggcc ggagagcttg acgctgctcg
     3421 gcgcggcggg ggcgaagcct gcgctcgaag ccctgcggcg aatgtccgac gcggcgttcc
     3481 cgctcgaccg cgggaagatc gaggcgcggc agtgggactg gaagacggcg ctacggttcc
     3541 cgctcgcgct cgacgaagaa gtgtacggcc tcggcgtgga cttcaggacg atgcggcgca
     3601 caggctcggt cttcctcctc cacgtcgatc actggggcgg gcagaccggt cgcacgcacg
     3661 cgcccgtgcc gctctatgtc tcgacgcgcg gctacgccgt cctcttcaac accgcgcgct
     3721 atctgaaggt gaatgtcggg cacggcgtgc ggctcgccgc cgaagataag ccg
//
LOCUS       DBNGOH010000515         2087 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3742144, whole genome shotgun sequence.
ACCESSION   DBNGOH010000515 DBNGOH010000000
VERSION     DBNGOH010000515.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2087)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2087)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2087
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3742144"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..431)
                     /locus_tag="AC159P_10075"
     CDS             complement(<1..431)
                     /locus_tag="AC159P_10075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolB family protein"
                     /protein_id="HIR3695840.1"
                     /translation="MNAQAGRFAVVLTIVAAAAVCLVAHVAHTENGTLRRVTETPAER
                     VSLNPSISGDGRKVAFESNALSAGPANASSFGAYVVNIDSAPAPLVEVARSRAPAPAV
                     SQDGSRIAFAGTSDLTGENRDGNSEIFYFDGAALRQLTDTRA"
     gene            complement(824..>2087)
                     /locus_tag="AC159P_10080"
     CDS             complement(824..>2087)
                     /locus_tag="AC159P_10080"
                     /EC_number="1.5.1.20"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional homocysteine
                     S-methyltransferase/methylenetetrahydrofolate reductase"
                     /protein_id="HIR3695841.1"
                     /translation="VTVLTAVEKMRLVTTKKLSAQPNAGLPRDVQGRQFYMCSPEYMA
                     KYAKRLIQAGVKFIGGCCGTTPAHIKLISDAVRAVSPRLLRSAARQTEKPARVVELTP
                     PDIQVVPLEERSNWARRIARGEFVTTVEVLPPKGCDAAKTLEQISLLKGAGVNAVNIP
                     DGPRAQTRMGAQATAVLVERELGLETVLHYCCRDRNLLGMMSDILGAAALGLRNLLLI
                     TGDPPKMGPYPDATAVFDIDSIGLTNMVNKLNHGLDLGNNPIGCPTSFCIGVGVNPGA
                     LNLDEEIKRFEWKVEAGAEFAITQPVFDVGQLRDFLKRVEHVRIPVVAGIWPLVSFRN
                     AEFLHNEVPGVRVTPEIMERMRLASDKGKEFARDEGLQIARESLLEVRELIQGVQVSA
                     PFNNVRYALEVFEALPDFAARKEAVTLN"
BASE COUNT          340 a          631 c          741 g          375 t
ORIGIN      
        1 gcgcgcgtgt cggtcaactg ccgcagggcc gcgccgtcga agtaaaagat ttcggagttg
       61 ccgtcgcggt tctcgcccgt gagatcactc gtgccggcga acgcgatcct cgaaccgtcc
      121 tgcgagacgg cgggcgcggg ggcgcgcgag cgcgcgacct cgacgagagg agcgggcgcg
      181 gagtcgatgt tcaccacata cgcgccgaac gaagaggcgt tggcagggcc tgccgaaagg
      241 gcgttggact cgaacgcgac cttgcgcccg tcgccgctga tcgaagggtt gaggctgacg
      301 cgctcggccg gcgtctcggt gacgcggcgc aacgttccgt tctcggtgtg cgcgacgtgg
      361 gcgacaaggc acaccgccgc ggcggcgacg atggtgagga cgacggcgaa ccgccccgct
      421 tgtgcgttca aggctctgct ccgttttctg ttcggggcgg ggcgacggaa gaggtcgagg
      481 ccagtggaag cgcacagagt ccggctgaat cagattcagc ggcggaataa accttactcg
      541 tcctgtgcgt tcagcttcgg cggcttttcg taggttgagg ggcgccgcgt gcgtctgttt
      601 gggcgggccg tattctacaa cttcccgcac gaatggcaac aaaaaaagta ggcagtaggc
      661 ggtaagcagt caagacagaa gtcggaattc ggaatacagg agagcgaaga agggcgaaga
      721 ggcttggggc tttacctctc aagcctcttc gtctttccga attccgactt ccgacttctg
      781 tcttgactgc ctaccgccta ctgcctactg cctactgcct acttcagttg agcgtgaccg
      841 cttctttgcg ggcggcgaaa tcggggagcg cttcgaagac ttcgagggcg tagcggacgt
      901 tgttgaaggg cgcggagacc tgaacgccct gaatcaactc cctgacttcg aggagcgatt
      961 cgcgggcgat ctggaggccc tcgtcgcggg cgaactcttt gcccttgtcg gaggcgaggc
     1021 gcatgcgctc catgatctcg ggcgtgacgc gcacccccgg cacttcgttg tggaggaact
     1081 cggcgttgcg gaaggagacg agcggccaga tgcccgcgac gacggggatg cggacgtgct
     1141 cgacgcgctt caggaagtcg cgcagttggc ccacgtcgaa gaccggctgc gtgatggcga
     1201 actcggcgcc ggcctcgacc ttccactcga agcgcttgat ctcttcgtcg aggttgagcg
     1261 cgccggggtt gacgccgacg ccgatgcaga aggaggtcgg gcagccgatg gggttgttgc
     1321 cgaggtcgag gccgtggttg agcttgttga ccatgttcgt caacccgatg gagtcgatgt
     1381 cgaacacggc ggtggcgtcc gggtacggcc ccattttcgg cgggtcgccg gtgatgagca
     1441 gcaggttccg caggccgagc gccgcagcgc cgaggatgtc cgacatcatg ccgagcagat
     1501 tgcggtcgcg gcagcagtag tgaagcaccg tttcaagacc gagttcgcgc tcgaccagca
     1561 cggcggtggc ctgcgcgccc atgcgcgtct gcgcgcgcgg gccgtcgggg atgttcaccg
     1621 cgttgacgcc cgcgcccttc aagaggctga tctgttcgag cgtcttcgcc gcgtcgcagc
     1681 ctttgggcgg gagcacctcg acggtggtga cgaactcgcc gcgcgcgatg cgccgcgccc
     1741 agttcgagcg ctcttcgagc gggacgacct gtatgtcggg cggcgtcagc tcgacgacgc
     1801 gcgcgggctt ctccgtctgc cgcgcggcgg agcgcagcag gcgcggcgag acggcgcgca
     1861 cggcgtccga gatgagcttg atgtgcgcgg gcgtcgtgcc gcagcagccg ccgatgaact
     1921 tgacgcccgc ctgtatcaaa cgcttcgcgt acttcgccat gtactcgggc gagcacatgt
     1981 agaactgtcg cccctgcacg tcgcgcggga gtcccgcgtt gggctgcgcg gagagcttct
     2041 tcgtcgtcac gagccgcatc ttttcgacgg ccgtcagcac ggtgacg
//
LOCUS       DBNGOH010000516         2846 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3009070, whole genome shotgun sequence.
ACCESSION   DBNGOH010000516 DBNGOH010000000
VERSION     DBNGOH010000516.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2846)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2846)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2846
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3009070"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            150..1469
                     /locus_tag="AC159P_10085"
     CDS             150..1469
                     /locus_tag="AC159P_10085"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HIR3695842.1"
                     /translation="MSKVIVVVGAQWGDEGKGKIVDLLAERFDIVARYQGGHNAGHSV
                     RVGEKSYALHLIPSGIIHEGKTCVLGNGMVIDPKAFFEEADRLAEQGLEVTPERVKVS
                     ARAHLILPYHRALDHTSEERLGNEKVGTTLRGIGPAYEDKAGRRGIRVADATNREVLR
                     ARIERNIEDANLIISRYRGTTLDAGQVYDEVVPLVERLGPFIADTTHFLNRAVREGQT
                     VLVEGAQAALLDVDHGTYPFVTSSSTTSGGACVGLGLAPQRVTGVLGIVRTYTTRVGE
                     GPFPTEMVEGEDETGQLIRERGREYGVSTGRPRRCGWFDAFATRYAAEINGFDCVALT
                     KLDVLDTLDEIKVCVGYQLDGRTVESLPAVSKDLRRIEPIYETLPGWQTSTEGVTELA
                     ELPPAARAYVDYLSQQIGVPVGLVSTGPDRAHTILLRDSALGHWLED"
     gene            1526..1598
                     /locus_tag="AC159P_10090"
     tRNA            1526..1598
                     /locus_tag="AC159P_10090"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1559..1561,aa:Lys,seq:ttt)
     gene            1981..2703
                     /locus_tag="AC159P_10095"
     CDS             1981..2703
                     /locus_tag="AC159P_10095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695843.1"
                     /translation="MKRLLAITLLIIGVALFILVSSTRVGLKPSAQPAGASQTPATKP
                     SEAPAAPAQEPGPAGPDKDVCSLPAAQVPVGGDLKLGMTVEKVLSLFPGSGEDSEVRS
                     SLARPAGPLGVSSLVIRPDRYRSKEKFAGVSQITFTLLDGRVSTFSVGYNGPEWPHVD
                     EFVAKIVKGTELPAADAWQAYAGLDNQLKILTCPGFEIRVHAGGEGGNLNYVLMSDRE
                     AARTLKERRDKAREKAGQEPKP"
BASE COUNT          559 a          933 c          853 g          501 t
ORIGIN      
        1 ggcgcctgca gtttctactt catcgagagg ccggtgaggc tgggaaaagc gagctgacgg
       61 tttcgagttc ggggcttcgg gttccgagtt tcgggaccgg attcccgccc gccgcgcaac
      121 tcgaaactcc gaactcggaa ctcgaaacca tgtccaaagt aatcgtcgtc gtcggcgcgc
      181 agtggggcga cgagggcaag ggcaagatcg tcgatctgct cgcggagcgc ttcgacatcg
      241 tcgcgcgcta tcagggcggg cacaacgcgg gccactcggt gcgcgtcggc gagaagtctt
      301 acgcgctgca cctgatcccc tcgggcatca tccacgaggg caagacgtgc gtcctcggca
      361 acggaatggt gatcgacccg aaggcgttct tcgaggaggc cgaccgcctc gccgagcagg
      421 gtctcgaagt cacgcccgag cgcgtcaagg tcagcgcacg cgcgcacctc atcctcccct
      481 accaccgcgc gctcgaccac acgagcgaag agcggctcgg caacgagaag gtcggcacca
      541 ccctgcgcgg catcggcccc gcctacgagg acaaggccgg aaggcgcggc atccgcgtcg
      601 ccgacgcgac gaaccgcgag gtgctgcgcg cgcgcatcga gcgcaacatc gaggacgcca
      661 acctcatcat cagccgctac cgcggcacga cgctcgacgc cgggcaggtc tacgacgagg
      721 tcgtcccgct cgtcgaacgg ctcggcccct tcatcgccga caccacgcac ttcctcaacc
      781 gcgcggtgcg cgaagggcag accgtcctcg tcgaaggcgc gcaggccgcg ctcctcgacg
      841 ttgaccatgg cacctatcct ttcgtcacct cctcttcgac cacttcgggc ggcgcctgcg
      901 tcggtctggg cctcgcgccg cagcgcgtca ccggcgtgct cggcatcgtc cgcacctaca
      961 ccacgcgcgt cggcgaaggc cccttcccca cagagatggt cgagggcgag gacgagacgg
     1021 ggcagctcat ccgcgagcgc gggcgcgagt acggcgtctc gaccggaagg ccccgccgct
     1081 gcggctggtt cgacgccttc gccacgcgct acgccgccga gatcaacggc ttcgactgcg
     1141 tcgcgctcac gaagctcgac gtgctcgaca cgctcgacga gatcaaggtc tgcgtcggct
     1201 accagctcga cgggcgcacc gtcgagagcc tccccgccgt ctccaaagac ctccgccgca
     1261 tcgaacccat ctacgagacg ctccccggct ggcagacctc caccgagggc gtcaccgaac
     1321 tcgcggaact gccccccgcg gcgcgcgcct acgtcgatta cctctcgcaa cagatcggcg
     1381 tccccgtcgg cctcgtctcg accggccccg accgcgcaca caccatcctg ctgcgcgact
     1441 ccgccctcgg ccactggctc gaagattaac ggcggaaggc gcgcttgtca ctcacccgcc
     1501 ccgaatgtaa aatgcaactc accaagggcc gatagctcag ttggtagagc aagtggcttt
     1561 taaccactgg gtcgcaggtt cgagtcctgc tcggctcatc aggtgcagta gatacagggc
     1621 ttgttggatt cctttgatta ctacttacgt cgtttgacta agtaggttgg aggttccaat
     1681 gagtccttta tcttctctgt ttgagcagtt cctgcaagga aaacgctgca tcaagaacat
     1741 tacccgccag actgcacagt tctaccgcac gtcgtggcag tcctatgtga agcggtgcgg
     1801 ggaggcccaa cttacaaaag ctcagttata gcggggttca ttcgtatgcg acttccgcca
     1861 aatgccccat cttacgctcg tttctagcga acacggcggc aaactgccag catcaggtcg
     1921 catccgaatg accaccgctg tagctggctt cggccaggcg gaaaactgaa ggggtaaggt
     1981 atgaaacgac ttctcgcaat cacgctgtta ataatcggcg tggctttgtt cattctcgta
     2041 agctcaaccc gggtcgggct aaagccttcg gcccaaccgg caggcgccag tcagactccc
     2101 gcaacaaagc cgagtgaagc gcctgccgcg ccggcacagg agcctggccc cgcaggcccc
     2161 gataaagacg tttgcagcct ccccgcggcg caggttccgg tgggaggcga cctcaaactg
     2221 gggatgaccg tggagaaagt gctgtcacta ttccccggca gcggcgaaga ttcggaagtc
     2281 cgttccagcc tcgccaggcc ggccggcccg ctcggggtgt ccagcttggt catcaggccg
     2341 gacaggtatc ggtcgaaaga gaagttcgcc ggagtcagtc agatcacatt cacccttctc
     2401 gacggtcgcg tctctacctt ctccgtcggc tacaacggcc ccgaatggcc acacgttgat
     2461 gaattcgtgg cgaagattgt caaggggacg gaacttccgg cggcggatgc gtggcaggcg
     2521 tacgccgggc tggacaatca attaaagatt ctcacgtgtc cgggcttcga gattcgtgtc
     2581 cacgccggcg gcgaaggcgg taatctcaat tatgtcctga tgagtgatcg ggaggcggcg
     2641 cggacgttaa aggagcgtcg ggataaagcc agagagaaag caggtcagga gcccaagcca
     2701 tagtcgtcca aggtctgacg cgccggctaa catcgatatg cagcggacgc ggcggtcttc
     2761 gtgccgcctc tcatcgtgtt cttgtattca taacaaaaaa ataatcgact tgtaacttgt
     2821 ttacctccga ctgtgtaata acaatg
//
LOCUS       DBNGOH010000517         2999 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4475413, whole genome shotgun sequence.
ACCESSION   DBNGOH010000517 DBNGOH010000000
VERSION     DBNGOH010000517.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2999)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2999)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2999
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4475413"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(118..1665)
                     /gene="gpmI"
                     /locus_tag="AC159P_10100"
     CDS             complement(118..1665)
                     /gene="gpmI"
                     /locus_tag="AC159P_10100"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003540614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HIR3695844.1"
                     /translation="MNSNRGPLALIILDGFGYTPEREGNAVALAATPFYDEILDKYPH
                     TLIEASGTCVGLPGGVMGNSEVGHLNMGSGRVIMTDIRRIDYAVETGAFFENEAFVTA
                     MDNARARGGALHLMGLLSDGLVHSSQQHLYALLRMAKQRGLQRVYVHCFLDGRDTPPS
                     SADRYVAALQNRITEIGCGEIASIVGRYYAMDRDKRWERTERAYNLLVRAEGERTHDP
                     IDAVKRSYERGVTDEFVEPIIVTRDDGTPVATIEDGDSVIFFNFRADRARQLTRALAV
                     PDFDGFAVLARPRTDFVCFTLYDKTFPLPIAFPQLQHNNILAEVFAAVGVRNYRLAET
                     EKYAHVTYFFNGGAEREFPHERRLLVPSPKVATYDLAPEMSAFKITDKLLRAIEEGDT
                     DVFIVNYANPDMVGHTGSLPKTIEAVQYVDTCLGWITKALKTARGTCLITADHGNCEQ
                     MLDPTTGQPHTAHTTNPVPFHLIGEHLTGVKLRDGGALEDVAPTILGLLDLDKPADMT
                     GRDLRDL"
     gene            complement(1729..1956)
                     /locus_tag="AC159P_10105"
     CDS             complement(1729..1956)
                     /locus_tag="AC159P_10105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HIR3695845.1"
                     /translation="MFFIFIIGTRFFTWGSEPGAQTTRCGQCGTVGVFTHKKGMRFVT
                     LFFIIPVLPISGVKHLLQCPNCGTRYETSSS"
     gene            complement(2094..2999)
                     /gene="eno"
                     /locus_tag="AC159P_10110"
                     /pseudo
     CDS             complement(2094..2999)
                     /gene="eno"
                     /locus_tag="AC159P_10110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014454767.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
BASE COUNT          465 a          849 c         1112 g          573 t
ORIGIN      
        1 ggcttgtccc tgcccgcgga tgcgatagat tcaacgtcga agataatgta acggcaggca
       61 gggacaagcc ctgcccctac aggattcggg atgtaccgtg ccaatgtgaa actttcttta
      121 gaggtcgcgg aggtcgcgtc cggtcatgtc ggcgggtttg tcgaggtcga ggaggccgag
      181 gatggtgggg gctacgtctt cgagggcgcc gccgtctctg agcttgacgc cggtgaggtg
      241 ttcgccgatg aggtggaagg ggacggggtt ggtggtgtgt gcggtgtggg gttggccggt
      301 ggtggggtcg agcatctgtt cgcagttgcc gtggtcggcg gtgatgaggc aagtgccgcg
      361 ggcggtcttg agcgctttgg tgatccagcc gaggcaggtg tctacgtatt ggacggcttc
      421 gatggtcttg gggaggctcc cggtgtggcc gaccatgtcg gggttggcgt agttgacgat
      481 gaagacatcc gtatcgcctt cttcgatggc gcgcagaagt ttgtcggtga tcttgaaggc
      541 ggacatctcg ggcgctaaat cataggtggc gactttgggg gaggggacga gcaggcgtcg
      601 ctcgtgcggg aactcgcgtt cggcgccgcc gttgaagaag taggtgacgt gggcgtactt
      661 ctcggtctcg gcgaggcggt agttgcgcac cccgacggcg gcgaagactt cggcgaggat
      721 gttgttgtgc tgtagctggg ggaaggcgat ggggagcggg aaggttttgt cgtagagcgt
      781 gaagcagacg aagtccgtgc gcgggcgggc gaggactgcg aagccgtcga agtcggggac
      841 ggcgagggcg cgcgtgagct ggcgggcgcg gtcggcgcgg aagttgaaga agatgacgga
      901 gtcgccgtct tcgatcgtcg cgacgggagt gccgtcgtcg cgcgtgacga tgatcggctc
      961 gacgaactcg tccgtgacgc cgcgttcgta ggagcgcttg acggcgtcga tggggtcgtg
     1021 cgtgcgctcg ccttcggcgc ggacgaggag gttgtaggcg cgctcggttc gctcccagcg
     1081 cttgtcccta tccatcgcgt agtagcgacc gacgattgaa gcgatctcgc cgcagccgat
     1141 ctccgtgatt ctgttttgca gcgcggcgac gtagcggtcg gcggaagagg gcggcgtgtc
     1201 gcgcccgtcg aggaagcagt ggacgtagac cctctggagg ccgcgctgtt tcgccatccg
     1261 cagcaacgcg tagaggtgct gctgcgagga gtggacgagg ccgtccgaga gcaggcccat
     1321 gaggtggagc gcgccgccgc gcgcgcgcgc gttgtccatc gcggtgacga aggcttcgtt
     1381 ctcgaagaag gctccggtct cgaccgcgta gtcgatgcgg cggatgtccg tcatgatgac
     1441 gcggcccgaa cccatgttga ggtggccgac ctcggagttg cccatcacgc cgccgggcag
     1501 gccgacgcag gtacccgacg cctcgatcaa agtgtgcggg tacttgtcca gaatctcgtc
     1561 gtagaaaggc gtcgcggcga gcgcgacggc gttgccctcg cgctcgggcg tgtagccgaa
     1621 gccgtcgagg atgatgagcg cgagcggtcc acggttcgag ttcaaaggaa tgcctcctcg
     1681 gaaaaaatcg cggcgcgaaa aagggcgcgg cccttcagcg cggccccttc agctcgaaga
     1741 tgtctcgtag cgcgtgccgc agttcgggca ctgtagcaga tgtttgacgc cgctgatggg
     1801 cagcacaggg atgatgaaga agagcgtgac gaagcgcatc cccttcttgt gggtgaagac
     1861 gcccaccgtc ccgcactgcc cgcagcgcgt cgtctgcgcg cccggctcgg acccccaggt
     1921 gaaaaaccgc gtcccgatga tgaagatgaa aaacaacggc gccctcttca gcgtcgcgcc
     1981 gcgcgcgcct tcttcgactt gccgccggtc ttcgtcgcgt cggtcgtctt agccgcgccg
     2041 ctcgtcttcg tcgtcgcggc cttcgccgcg gggcgcgcgg cgggcgctgc gatctggtgg
     2101 aaggccgagc ggccggggta gcgcgcggcg ccgcgcaggt cttcctcgat gcgaaggagc
     2161 tggttgtact tggcggtgcg gtcggtgcgc gagagcgagc cggtcttgat ctggccggcg
     2221 ttggtggcga cggcgaggtc tgcgatgaag gtgtcctcgg tctcgcccga gcggtgcgag
     2281 atgacggcgg tgcgcccgtt ggtgcgcgcc agctcgatgg cgtcgagcgt ctccgtgagc
     2341 gtgccgatct ggttgacctt gatgaggatg gaattggcga cgccgagtgc gatgcccttc
     2401 cgtaagaatt ccgtgttggt gacgaagagg tcgtcgccga cgagctggat gcgcgagccc
     2461 atctcgtcgg tcatcagctt ccagcccgtc cagtcgttct cggccatccc gtcctcgatg
     2521 gagatgatgg ggtactgctc cgcccacgtc ttccagtagg cgaccatctc ctcggatgag
     2581 agcctgcggc cgtccgactt cttgaagaca tagttcttgc cgtcgtagaa ctcgctggcc
     2641 gccgggtcta acgcgagcat gacgttgacg cccgcgttat agcccgccgc cgtgacggcc
     2701 tccaggatgg tctcgacggc ctcctcgttc gagcgcaggt cgggcgcgaa gccgccctcg
     2761 tcgccgacgc ccgtcgagag gccgcgcccc ttcagcacag acttcagcgc gtgaaaaatc
     2821 tcggcgcccg tgcgcagcgc ttcgcggaag ctttgggcgc cgacggggac gaccatgaac
     2881 tcctgaaagt ccacgttgtt gtcggcgtgc gcgccgccgt tgaggatgtt catcatcggc
     2941 acgggcaagg tcttcgcgtt ggcgccgccg agataacgat agagcgggat ttcgaggtg
//
LOCUS       DBNGOH010000518         2653 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4475429, whole genome shotgun sequence.
ACCESSION   DBNGOH010000518 DBNGOH010000000
VERSION     DBNGOH010000518.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2653)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2653)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2653
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4475429"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..854)
                     /locus_tag="AC159P_10115"
     CDS             complement(<1..854)
                     /locus_tag="AC159P_10115"
                     /inference="COORDINATES: protein motif:HMM:NF015891.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HIR3695846.1"
                     /translation="MKRGIMPVVKNKALLAVAAALLCAAAANAQPQQQPTPTPAPDDG
                     PVSSKSYRSRVFEIKHRDPRNLLNVLTPLGSGFKGTAMSYNEEFKTITIRDFPENIAT
                     VEEAIKRLDVPEPARPGVEFHVHILVATNRPAGATPDQYPDELGTVIKQLQSTINYKH
                     YSLLTSQVLRAKEQGPRHVSVKGVSELKLRADTEASRNPIFYEFRFDNITLDVPTSGA
                     AKIQIGLFAFSMKVPLLVSAGTGALQYQDIGFHTPVNMREGEKVVVGTTSMEDKGIVV
                     VLTGAITK"
     gene            complement(876..1610)
                     /locus_tag="AC159P_10120"
     CDS             complement(876..1610)
                     /locus_tag="AC159P_10120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor family protein"
                     /protein_id="HIR3695847.1"
                     /translation="MDCKRAEELMPRDAGGDLEDGRARAGLSAHLGDCAPCRRLAAEW
                     AESRALLRLHEPPEFDAAFFEHVRRNVMREITTPTTAAPAPTVFAALLALLPRARTLA
                     YAASLALVFAALLFGLRLSRAPQPTKVAGGGVASSVNEALAKEINPPRPTPRIVVREQ
                     AHRKTRAFKTVPRADRPAPTRAAALPDAAALSSLPDAAAELAVVRDEREMLKIELQTR
                     DPNVRIIWFSPQPSEQTSPRRSNDQR"
     gene            complement(1627..2253)
                     /locus_tag="AC159P_10125"
     CDS             complement(1627..2253)
                     /locus_tag="AC159P_10125"
                     /inference="COORDINATES: protein motif:HMM:NF019265.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR3695848.1"
                     /translation="MAENPTPGGAAAEGVVAALSALAERARAGDAAAFEQLMVCTQHK
                     VAATAWRLLGSREDARDATQESYLRAYKYLKSYRPGQDFHGWLYRITVNVCRDMMRTR
                     RGAERFTHATSHDAGRERGALEAVACEADTEEAAVLAQKRAIVRRALETLPEKERTAV
                     VLRDLEGLSTEEVARLMKTRPATVRSQVSTARTKLKLYCERFINRRRD"
BASE COUNT          377 a          876 c          916 g          484 t
ORIGIN      
        1 cacttcgtga tggcgcccgt gagcacgacg acgatgcctt tgtcctccat gctcgtcgtg
       61 ccgacgacga ccttctcgcc ctcgcgcatg ttgacgggcg tgtggaagcc gatgtcctgg
      121 tactggagcg cgcccgtgcc ggcgctgacg aggagcggca ccttcatcga gaaggcgaac
      181 agcccgatct ggatttttgc ggcgcccgac gtgggcacgt cgagcgtgat gttatcgaag
      241 cggaactcgt agaagatcgg gttcctgctc gcctccgtgt cggcgcgcag cttcagctcc
      301 gagacgccct tcacgctgac atgtcgcggc ccctgctcct tcgcgcggag cacctgcgag
      361 gtgagcagac tgtagtgctt gtaattgatc gtggactgga gctgtttgat gacggtgccc
      421 agctcgtccg ggtattggtc gggggtggcg cccgcgggcc tgttcgtcgc gacgaggatg
      481 tggacatgaa actcgacgcc gggccgcgcc ggctccggca cgtcgagccg tttgatggcc
      541 tcctccacgg tggcgatgtt ctcggggaag tcgcggatgg tgatcgtctt gaactcctcg
      601 ttgtagctca tggccgtccc cttgaagccg ctgccgagcg gcgtcagcac gttcaagagg
      661 ttgcgcgggt cgcggtgctt gatctcgaag acgcggctgc ggtagctctt gctgctgacg
      721 gggccgtcgt cgggcgcggg cgtcggggtg ggttgctgct gcggctgcgc gttcgcggcg
      781 gcggcacaga gcagcgcggc ggcgacggca agtagtgctt tgttcttcac gacgggcatg
      841 attcctcttt tcattgcgga catgattcct cctgactaac gttggtcgtt cgatctgcgg
      901 ggcgaagtct gttcgctcgg ctgcggcgag aaccagatga tgcgcacgtt cgggtcgcgc
      961 gtctgcaact cgatcttcag catctcgcgc tcgtccctga cgaccgccag ctccgcggca
     1021 gcgtcgggga gagacgacag ggccgcggcg tccggaagcg ccgccgcgcg agtcggcgcg
     1081 gggcggtctg cgcgcgggac ggttttgaac gcccgcgtct ttcggtgcgc ctgctcccgc
     1141 acgacgatgc gaggcgttgg ccgcggcggg ttgatctctt tcgctaaagc ttcgttcacg
     1201 ctcgacgcga cgccgccgcc cgcgaccttc gttggctgcg gggcgcgcga aaggcggagg
     1261 ccgaagagca acgccgcgaa gacaagggcg agcgaggccg cgtaggccag cgtgcgcgca
     1321 cgcgggagca gcgcgagcag ggcggcgaag acggtcggcg cgggcgccgc ggtcgtcggc
     1381 gtcgtgatct cgcgcatcac gttgcggcgg acgtgttcga aaaaggcggc gtcgaattcg
     1441 ggcggctcgt gcagacgcag cagcgcacgg ctctcggccc actcggcggc gaggcggcgg
     1501 cacggggcgc agtcgccgag gtgcgctgac agcccggcgc gcgcgcgccc gtcttcgagg
     1561 tcgcccccgg cgtcgcgagg catcagttct tcggcccgtt tgcagtccat cggtgttcgg
     1621 cctcctctag tcgcggcgcc tgttgatgaa gcgctcgcag tagagcttga gcttcgtgcg
     1681 cgccgtcgag acctgcgagc gcaccgtcgc gggccgcgtc ttcatcagcc gcgcgacctc
     1741 ctcggtcgaa agtccttcga ggtcccgcag cacgaccgcc gtgcgctcct tctccggcag
     1801 cgtttcgagc gcgcgccgca cgatggcgcg cttctgcgcg agcaccgccg cctcttccgt
     1861 gtccgcctcg cacgccaccg cttcgagcgc gccacgctcg cgccccgcat catgcgaggt
     1921 cgcgtgcgtg aaccgctccg ctccgcgccg cgtgcgcatc atgtcgcggc agacgttgac
     1981 cgtgatgcgg tagagccagc cgtggaagtc ctgccccggc ctgtagctct tcaggtactt
     2041 gtaggcgcgc aggtatgact cctgcgtcgc gtcgcgcgcg tcctcgcggc tgccgagcag
     2101 gcgccacgcg gtcgcggcca ccttgtgctg cgtgcagacc atgagctgct cgaaggccgc
     2161 cgcgtcccct gcgcgcgcgc gctcggccag tgccgagaga gcagccacga cgccctccgc
     2221 cgccgcgccg ccgggcgtcg gattctcggc catgaaagcg acactcgcga cctcgacggg
     2281 catactccgc gtcagacctc tcttcggctc cccacgcata caactacctt cgtcgggcgc
     2341 ataacgttga gaactttttg gcgcccgagc gccagccgcc ctcagaccta cacgcgcgcc
     2401 ctcgtcgaag gttgcaaaga gagtgtgcag ctgagaagcg gtaggcggta ggcggtaggc
     2461 agtaaaagac agaggtcgga ggtcggaggt cggagatcag caaaagcgag gggcttgaga
     2521 cgttagctct caagcccctc gcttcatcgc ttgaagaagg aggaggctcg ggaggtaaag
     2581 tcccgagcct cctcgccctt cttccgactc ctgacttccg acttctgtct tttactgcct
     2641 actgcctact gcc
//
LOCUS       DBNGOH010000519         6847 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1177914, whole genome shotgun sequence.
ACCESSION   DBNGOH010000519 DBNGOH010000000
VERSION     DBNGOH010000519.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6847)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6847)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6847
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1177914"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            447..2945
                     /locus_tag="AC159P_10130"
     CDS             447..2945
                     /locus_tag="AC159P_10130"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000225 -
                     N-acetylglucosaminylphosphatidylinositol deacetylase
                     activity [Evidence IEA];
                     GO_process: GO:0006506 - GPI anchor biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HIR3695849.1"
                     /translation="MKKLSSLAALALLLSALASASAQAPAVMDAAEIQLALKRMTVVG
                     SALYVGAHPDDENTALLAYLANERGVRAAYLSVTRGDGGQNLIGTEQGAALGLVRTQE
                     LLAARRLDGAEQFFTRAIDFGFSKSPAEALRVWGHEAALADVVWVVRRFRPDLVITRF
                     PTTGEGGHGHHTASAILAREAFEAAADPKRFPEQLAYVKPWRPKRLVWNAFVRQGERP
                     ANADKLLSVDVGAFNPLLGQSYTEIAARSRSMHKSQGMGAAERRGTALNYFAHVAGEE
                     AGDDLFEGVDMTWGRVAGGEAAGRLLEQAGRDFRPSNPRAILPLLLRVRAELRKLAAD
                     DPLVNAKRDELSKLILDSTGLWAEAVAAEAYTTPGADVRVAVTLVNRSDVPLRFEGVT
                     LSGGTPQAVGKELKNNQPLTTELKVPAPAEYSQPYWLRKEPGAGLFAVEGQENVGEPV
                     AASQLTLVVKATAGENEEIDFTLPVLFRWTDRVQGELYRPVVVVPEVAVRLEEKVLVY
                     SDRRPKLVRVKLKSNVAGDASGTLRLRLPAGWAAAPSEVPVTLKGKGTEAAATFNVTP
                     PADGSTATLSAEFETGGKKFTHGMVEIDYPHIPRQTLFPVAAARLVRVELARRGARVG
                     YVMGAGDEVPEALRQVGYDVTLLSDEDLDAADFSRFDAVVTGVRAYNTRATLRKQQQL
                     LLEYVERGGTLVVQYNTPERALEGFAFGPYPLRLSSERVSVEDAPVTLLAARDELRNE
                     LLDAPNKITSSDFDGWVQERGLSFPSEWDARYGTLFESHDPGESAKPGGTLVARHGKG
                     TYVYTSYSWFRQLPAGVPGAYRVFVNLISAGKKE"
     gene            2942..3133
                     /locus_tag="AC159P_10135"
     CDS             2942..3133
                     /locus_tag="AC159P_10135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695850.1"
                     /translation="MSGDPLRSVRARDGASRATDDERPRATDESPPFGRSWAVLYAAV
                     LLNLALLVLLFYLFTRAFR"
     gene            3130..4830
                     /locus_tag="AC159P_10140"
     CDS             3130..4830
                     /locus_tag="AC159P_10140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019987030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HIR3695851.1"
                     /translation="MRPLDWLVLAAALVGIVLYGLWKGRRTRDLDDFLVADRRMRWHH
                     VALSIMATQASAITFLSTPGQAFADGMRFVQFYLGLPVAMVILSITAVPLYHRLKVYT
                     AYEYLEGRFDLKTRTLAALLFLALRGLSTGVSIYAPSVILSVIMGWDIRVVIFLIGGM
                     VLLYAARGGARAVDNTNFQQFLIIMGGMFVAFLTIIWLLPADVSFVEALHVTGKLGRL
                     NAIDFTFDPSNRYNFWSGLVGGCFIALSYFGTDQSQVQRYLTGESVAQSRLGLLFNGM
                     VKVPMQFFILFLGAMVFAFYQFTQPPIFFNPVELEKARAGQHAEQFRALEAEHAAAFD
                     AKQRAAREYIGALRAGDTAATETAARGLLAAHDKSNEVRAGAVDVLKQYDKNPNDTNY
                     VFLNFVTHYLPAGVVGLVLAAVFFASMSSTASEWSALASTTVVDIQRRLVRRQKGDRY
                     YLVSSKLATLFWGLFAVTFAQYAGQLGSLVEAVNRLGSLFYGTLLGIFLLAFYFKRTG
                     GTAAFVGALAGELVVLYCFFRTDIAFLWYNVIGAFTVIVVALVVAVGSGQLRQKSKEA
                     "
     gene            complement(4927..5880)
                     /locus_tag="AC159P_10145"
     CDS             complement(4927..5880)
                     /locus_tag="AC159P_10145"
                     /inference="COORDINATES: protein motif:HMM:NF022588.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HIR3695852.1"
                     /translation="MKPAQTFSRRAVALSLFLLVAAPVCFAQSSLRLPRPSQKATVMQ
                     TVGVTDVSITYSRPGVKGRKIWGDPPAGTAAGEATLDDARARPKDAVIVPYGRVWRAG
                     ANEATLFKVTDDVLVNGQPLPAGSYSLHAVPGPAEWTIIFNKDDGQWGSFSYDAAKDA
                     LRVKTKPETTADTQEWLSYSIEPEKENAARVNLRWERLRVPFTVEVKDVNALTLAKAR
                     AAIAAAKPDDWQTPLQAANYSLNNKLDLDEAMRWAEHSIKAKENYNNLNLKARILAAQ
                     GKTADAIATGEKALQVGKAANANAQAMANFEKTLAEWKAKK"
     gene            6318..>6847
                     /locus_tag="AC159P_10150"
     CDS             6318..>6847
                     /locus_tag="AC159P_10150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695853.1"
                     /translation="MAKGNPGADSSSPVTDDLINTATQQFGQAPLFWGRYFTSPTASG
                     SVEYRHGSESPILAQHNIRLLPVARQTAHVGGSQQQGTSDAQLNVQDVLDTFGQDYLA
                     SQGGQFLLFLDVEGSPSTGSPSLSLDYYLGWGQTLVSYSRSRTGNAVTVLPCVYARQG
                     DNQTWNNLVSANGQGVT"
BASE COUNT         1050 a         2297 c         2264 g         1236 t
ORIGIN      
        1 cactcaaccg ccatcccctc tggaggggcg cggcgagaac gatttctcat ttcggccaca
       61 attctgtttc tgctgctcgc actatatgtg caaccccgca tcggctataa tccacccttc
      121 attatcacgc cacgctcggc ggcttaccga atccatgaga cgcgattcct cgcccttcgg
      181 gtagcgcgcc aatgcgcgcc cgaagtagtc ctgcttctgc gtccgccgac attcgaaggt
      241 tcgacctgcg aagtcgcacg gcgcggcggg catccaaagt cgagtgaagt attgcgggcg
      301 ggccgacaaa tcattcgcac cgagagcggg cgcgcccctc ggaacgactc tttttacaac
      361 taccctgttg accagccgtc cgactcttcg gaacaatggt ggctcccgat ccgtgattgc
      421 gaaactcgct ccgtgaaccg tcgaacatga agaagctgtc gtccctcgcc gccctcgcac
      481 tactgctgtc ggccctcgcg tccgcgagcg cccaggcccc cgccgtgatg gacgcggcgg
      541 agatacagct cgcgctcaag aggatgacgg tcgtcggcag cgcgctctac gtcggcgcac
      601 acccggacga cgagaacacg gcgctgctgg cgtatctggc gaacgagcgg ggcgtgcgcg
      661 cggcctacct ctcggtgacg cgcggcgacg gcgggcagaa cctgatcggc acggagcagg
      721 gcgcggcgct cggcctcgtc cgcacgcagg agcttttggc ggcgcggcgc ctcgacggcg
      781 cggagcagtt cttcacgcgc gccatcgact tcggcttctc gaagtcgccc gcggaggctt
      841 tgcgcgtgtg gggccacgag gcggcgctgg cggacgtggt gtgggtcgtc cgtcgcttcc
      901 gcccagacct cgtcatcacg cgcttcccca ctacgggtga gggcgggcac ggccaccaca
      961 ccgcctcggc catcctcgcg cgcgaggcgt tcgaggcggc tgccgacccc aagcgcttcc
     1021 ccgaacagct cgcatacgtc aagccgtggc ggcccaagcg gcttgtctgg aacgccttcg
     1081 tccggcaggg cgagcggccc gcgaacgccg acaagctgct gagcgtggac gtgggcgcct
     1141 tcaaccctct gctcggccag tcctacacgg agatcgcggc gcggagccgc tcgatgcaca
     1201 agagtcaggg gatgggggcg gcggagcggc gcggcaccgc gctcaactac ttcgcgcacg
     1261 tggcgggcga agaggcgggc gatgacctct tcgagggcgt ggacatgact tgggggcgcg
     1321 tggcgggcgg cgaggcggcg ggccggctgc tcgaacaggc ggggcgcgat ttccgcccgt
     1381 cgaacccgcg cgccatcctg ccgctgctct tgcgcgtgcg ggccgagctg cgcaagctcg
     1441 cggcggacga cccgctcgtc aacgcgaagc gcgacgagct gtcgaaactg attctcgact
     1501 ccacggggct gtgggccgag gccgtcgccg ccgaagccta cacgacgccg ggcgcggacg
     1561 tgcgcgtcgc cgtcacgctc gtcaatcgtt ccgatgtgcc gctgcgcttc gagggcgtca
     1621 cgctctccgg cggcacgccg caggcggtgg gcaaggagtt gaagaacaac cagcctttga
     1681 cgacggagct gaaggtgccg gcgcctgccg aatacagcca gccgtactgg ctgcggaaag
     1741 agccgggcgc ggggctgttc gcggtcgagg gacaggaaaa cgtcggcgag cctgtggcgg
     1801 cctcgcagct cacgctggtc gtgaaggcca cggctggcga gaacgaggag atcgatttca
     1861 cgctgcccgt cctcttccgc tggacggatc gcgtgcaggg agagctgtac cgcccggtcg
     1921 tcgtcgtccc ggaagtcgcc gtccgcctgg aagagaaggt gctcgtctat tccgaccgcc
     1981 gaccgaagct ggtgcgtgtg aagttgaaga gtaacgtggc gggcgacgcg tccggcacgc
     2041 tgcgcctgcg gctgcctgcc gggtgggccg ccgccccttc ggaagtcccc gtcacgctga
     2101 aagggaaggg cacagaggcg gcggcgacct tcaacgtcac gccgcccgca gacggttcga
     2161 ccgcgaccct ctccgccgag ttcgagacgg gcggcaagaa gttcacgcac gggatggtgg
     2221 agatcgacta cccgcacatc ccgcggcaga cgctgttccc cgtggcggcg gcgcggctcg
     2281 tgcgcgtcga gctggcgcgg cgcggcgcgc gcgtcggcta cgtcatgggc gcgggcgacg
     2341 aggtgccgga ggcgctccgg caggtcggct acgacgtgac gctcctctcg gacgaagacc
     2401 tcgacgcggc ggacttctcg cggttcgacg cggtcgtgac gggcgtgcgc gcatacaaca
     2461 cgcgcgcgac gctgcgaaag caacagcaac ttctgctcga atacgtcgag cgcggcggca
     2521 cgctcgtcgt gcagtacaac acgccggagc gcgctctcga aggtttcgcg ttcggcccct
     2581 acccgctcag gctctcgtcg gagcgcgttt cggtcgagga cgcgcccgtc acgctcctcg
     2641 cggcgcgcga cgagctgcgc aacgaattgc tcgacgcgcc gaacaaaatc acgtcgtccg
     2701 acttcgacgg ttgggtgcag gagcgcggcc tcagttttcc ctccgagtgg gacgcgcgct
     2761 acggcacgct cttcgagagc cacgaccccg gcgagtcggc caagcccggc ggcacgctcg
     2821 tcgcgcgcca cggcaagggc acctacgtct acacgtctta ttcgtggttc cgccaactgc
     2881 ccgcgggcgt gccgggcgcg taccgcgtgt tcgtcaacct catctcggcg ggaaagaaag
     2941 aatgagcggc gacccgttgc ggagtgtaag agcgcgcgac ggagcgtcgc gtgcgacgga
     3001 tgatgagagg ccgcgtgcga cggacgagtc gccgcccttc ggtcggagtt gggcggtgct
     3061 ctacgccgcg gtgttattga acctcgccct gctcgtcctg ctcttctacc tcttcacgcg
     3121 ggccttccga tgagaccact cgactggctg gtgctcgccg cggcgctcgt cggcatcgtc
     3181 ctctacggcc tgtggaaggg gcggcgcaca cgcgacctcg atgacttcct cgtcgcagac
     3241 cggcggatgc gctggcacca cgtcgcgctc tccatcatgg cgacgcaggc gtcggccatc
     3301 acgtttcttt cgacgccggg gcaagccttc gccgacggga tgcgcttcgt gcagttctac
     3361 ctcggcctgc ccgtggcgat ggtgattctt tccatcaccg ccgtcccgct ctatcaccgg
     3421 ctcaaggtct acaccgctta cgaatacctc gaaggccgct tcgatttgaa gacgcgcacg
     3481 ctcgccgcgc tgctcttcct cgcgctgcgc ggactctcga cgggggtctc gatctacgcg
     3541 ccgtcggtca tcctctcggt catcatgggc tgggacatcc gcgtggtgat tttcctgatc
     3601 ggcgggatgg tcttgctcta cgcggcgcgg ggcggcgcgc gcgccgtgga caatacgaac
     3661 ttccaacagt ttctcatcat catgggcggg atgttcgtcg ccttcctcac gatcatctgg
     3721 ctgcttccgg cggatgtgtc gttcgtcgaa gcgctgcacg tgacgggcaa gcttgggcga
     3781 ctcaacgcca tcgacttcac gttcgacccg tcgaaccgct acaacttctg gtcgggcttg
     3841 gtcggcggct gcttcatcgc gctctcctac ttcggcaccg atcagtcgca ggtgcagcgc
     3901 tacctgacgg gcgagtcggt cgcgcagagc cgtctgggcc tgctcttcaa cgggatggtc
     3961 aaggtgccga tgcagttctt catcctcttc ctcggcgcga tggtcttcgc gttctaccag
     4021 ttcacgcagc cgcccatctt cttcaacccg gtcgagcttg agaaggcgcg cgccggccaa
     4081 cacgccgaac aattccgcgc gctcgaagcc gagcacgcgg cggccttcga cgcgaagcag
     4141 cgcgcggcgc gggagtacat cggcgcgctg cgcgcgggcg atacggcggc gacagagacg
     4201 gcggcgcgcg ggctgctcgc ggcgcatgac aagtcgaacg aggtgcgcgc cggggccgtc
     4261 gatgttttga agcagtacga caagaatccc aacgacacca actacgtctt cctcaacttc
     4321 gtcacgcact acctgccggc tggcgtggtg ggtctcgtgc tggcggcggt cttcttcgcc
     4381 tcgatgtcct cgaccgcctc ggagtggagc gcgctcgcct cgacgaccgt cgtggacatc
     4441 cagcgccgcc tcgtccgccg gcagaagggc gaccgctact acctcgtctc ttcaaagctg
     4501 gcgaccctct tctggggact cttcgccgtc accttcgcgc agtacgccgg acagctcggc
     4561 tcgctcgtcg aggccgtcaa tcgcctcggc tcgctcttct acggcacgct gctgggcatc
     4621 ttcctcctcg ccttctactt caagcgcacg ggcggcaccg ccgccttcgt cggcgccctc
     4681 gcgggcgagc tggtcgtcct ctactgcttc ttccgcacag acatcgcctt cctctggtac
     4741 aacgtcatcg gcgccttcac cgtcatcgtc gtcgcgctcg ttgtagcagt gggcagcggg
     4801 cagttaagac aaaagagcaa ggaggcttga gaggtaaagt gccgggcctc cttgctcttc
     4861 aagcaataaa gcaagaggct tgggacttta cctcccaagc ctcttgcttc tactgcctgc
     4921 tgcccgctac ttcttcgctt tccactcggc gagcgtcttc tcgaagttgg ccatggcttg
     4981 cgcgttggcg ttggcggcct tgccgacttg gagcgccttc tcgccggtgg cgatggcgtc
     5041 ggcggtcttg ccctgtgcgg cgaggatgcg cgccttgagg ttcaggttgt tgtagttctc
     5101 tttggccttg atggagtgct cggcccagcg catcgcctcg tcgaggtcga gcttgttgtt
     5161 gagcgagtag ttggccgcct gcaagggcgt ctgccagtcg tcgggcttgg ccgcggcgat
     5221 ggcggcgcgg gccttcgcca gcgtcagcgc gttcacatcc ttgacctcga ccgtgaaggg
     5281 cacgcgcagg cgctcccagc gcaggttgac gcgcgcggcg ttctccttct ccggctcgat
     5341 gctgtaggag agccactcct gtgtgtcggc ggtcgtctcg ggcttggtct tgacccgcag
     5401 cgcgtccttc gcggcgtcgt aagagaagct gccccactgg ccgtcgtcct tgttgaagat
     5461 gatcgtccac tcggcggggc cggggacggc gtggaggctg tagcttccgg cgggaagcgg
     5521 ctggccgttg acgagcacgt cgtccgtgac tttgaacagc gtcgcctcgt tggcgcccgc
     5581 gcgccagacg cgaccgtaag ggacgatcac cgcgtccttc gggcgcgcgc gcgcgtcgtc
     5641 gagcgtcgcc tcgcccgcgg ctgtcccggc gggcgggtcg ccccagattt tgcgcccctt
     5701 cacgccgggg cggctgtagg tgatggaaac gtcggtgacg ccgacggtct gcatgacggt
     5761 cgccttctgg ctcggtcgcg gcagacgcag gctggattgc gcgaagcaga ccggcgcggc
     5821 gacgaggagg aagagcgaca gcgcgacggc gcgccgggag aacgtctgtg cgggtttcat
     5881 cctgatactc ctcatgtgtt gctgttcgag tgaatgccga ccgaaaatcg tcgtggctcg
     5941 cggcggtgcc gcccggcggg acggcgcgga tgactttcga tgtctttcgg cgattgcccg
     6001 atggtaacaa aaaaaggcgc gcgtgattct cacacagttg taaaaagttc gccccgcctt
     6061 cggtgcctct aagtgtgcga gtgcgagggc aagtcggcgt ggtcgcggga acagccgtta
     6121 aggtatgatg cctgccgcag gctctcttag ctttgctcgt cgggtctatt catgaatcct
     6181 cgcccctcat gcacgcggag aactttccgg cttgaagata attttcgcca cgtaagagac
     6241 cgcgcgccgg cccggcttcg acgaataacc gcgcgccttt ggcggctggc ttggtaaact
     6301 ttcctctgga ggagaaaatg gctaaaggta atcccggagc ggacagctcc tcgcccgtca
     6361 ccgacgacct catcaacacc gccacccagc agttcggaca ggcccccttg ttctgggggc
     6421 gctacttcac gagtccgacc gcttcgggga gcgtcgagta tcgccacggc tccgaaagcc
     6481 ccatcctcgc gcaacacaac atccgcctcc tcccggtcgc gcggcagacg gcgcacgtcg
     6541 gcggcagtca acagcagggc acctcggacg cgcagctcaa cgtacaggac gtgctcgaca
     6601 cgttcgggca agactacctc gcctcgcagg gcggccagtt cctcctcttc ctcgacgtgg
     6661 agggcagccc ttccacgggc agcccgtcgc tgtcgctcga ctattacctc gggtgggggc
     6721 agacgctcgt cagctacagc cggagccgga cgggcaacgc cgtcaccgtc ctgccctgcg
     6781 tctacgcgcg gcagggcgac aaccagacgt ggaacaatct ggtcagcgcg aacggtcagg
     6841 gcgtgac
//
LOCUS       DBNGOH010000520         3030 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_76773, whole genome shotgun sequence.
ACCESSION   DBNGOH010000520 DBNGOH010000000
VERSION     DBNGOH010000520.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3030)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3030)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3030
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_76773"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(127..1296)
                     /locus_tag="AC159P_10155"
     CDS             complement(127..1296)
                     /locus_tag="AC159P_10155"
                     /inference="COORDINATES: protein motif:HMM:NF042449.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase assembly
                     protein OpcA"
                     /protein_id="HIR3695854.1"
                     /translation="METQSSAVALSAAKGVDAVKLERELAAMWAEMGAGGQEAGSSSP
                     SAGVTRACALNLIVYSERQDERAEVDALLEEVVERHPCRSIVLYADRAGEEARLEAYV
                     STRCQLASRGAKRICGEQVTIEAGGPIVQTLSTAVSPLLVPDVPVFLWWKDIPHYEDK
                     LFNRLAQLSDRVVIDSASFDNPHEDMVRLAEILGKSAGRMRLSDLNWGRLTTWRSLVA
                     SFWDVADYRASLDRIERVVIEYDRPDRSPDEVSPKALLAIGWLGTQLGWEVAPEGHRV
                     GKRAAQFAMTAAGRAVVAELSGTADTKGRDGFIMSLALQCADGNEFFVRLRPIGAKLE
                     TGARTGGEHTVGRVLDFEARSEGRRLSAELDILTRDRVFEEAAGFAARLLGAAKR"
     gene            complement(1409..2953)
                     /gene="zwf"
                     /locus_tag="AC159P_10160"
     CDS             complement(1409..2953)
                     /gene="zwf"
                     /locus_tag="AC159P_10160"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HIR3695855.1"
                     /translation="MQAASTLDNPLREGVRLERTAEPCAVVIFGASGDLAKRKLIPAL
                     YRLVQERLLPAEFAIIGLGRTAMSDDEFREKMKASVIEFSEAKRVDEEVWQSFAQGIR
                     FLPSNIEKPECYQVLSEMLEQVDRERGTQGNRLFYLSVAPEFYAEAVRQLGEAGLTKQ
                     GKGWVRVIIEKPFGTSLDSARELNQQILKHLEEKQIYRIDHYLGKETVQNLLVFRFAN
                     GIFEPMWNRQYIDHVQITNAETVGVEGRGGYYEKSGVVRDMIQNHVFQVLSLVAMEPP
                     ATLGPEDVRDEKIKAMHSARAFSPERVRAECVRGQYGPGSIGGKPVPGYREEQGVAPD
                     SVTETYAMITMWFDNWRWSGVPFYIRSGKRLAKRVTEIAIQFKQAPHQLFGTGAMEFV
                     EPNQLVIRIQPDEGITMRFAAKVPGQVTRIRDVTMDFRYGASFGVQLAEAYERLILDC
                     ILGDSTLYARADMTERGWEIVEPILEEWGEHKETATFPNYEAGSWGPEEASRILEQNG
                     RRWRRP"
BASE COUNT          481 a         1057 c          965 g          527 t
ORIGIN      
        1 acgacggtgc tgccgagctg ctctccgtcc gtctcgatca gcgcgacgtt ggttttcgtg
       61 cccccgatgt ctccggcgat tatcatttcg ctcgaccgtt ctccacgacg ttgaagtacg
      121 aagtccctaa cgcttggccg ctccgagcag gcgcgcggcg aagcccgcgg cctcttcgaa
      181 gacgcggtcg cgcgtcagga tgtccagctc ggcgctgagg cggcgcccct cgctccgcgc
      241 ctcgaagtcg agcacgcgcc cgaccgtgtg ctcgccgccg gtgcgcgcgc cggtttcgag
      301 cttggcgccg atgggccgca ggcggacgaa gaactcgttg ccgtcggcgc actggagcgc
      361 gagcgacatg atgaagccgt cgcgcccctt cgtgtcggcg gtgcccgaca gttcggcgac
      421 gaccgcgcgg cccgccgccg tcatggcgaa ctgcgccgcg cgcttgccga cgcgatgccc
      481 ctccggcgcg acctcccacc cgagctgcgt gccgagccag ccgatggcca gcagcgcctt
      541 cggagagacc tcgtccggcg acctgtcggg ccggtcgtac tcgatgacga cgcgctcgat
      601 cctgtccagc gacgcgcggt agtcggccac atcccagaag ctcgcgacga gcgagcgcca
      661 cgtcgtcagc cggccccagt tcaaatcgct caggcgcatc cggcccgcgc tcttccccaa
      721 gatctcggcc agccgcacca tgtcttcgtg cgggttgtcg aacgaggccg agtcgatgac
      781 cacgcggtcg gagagctgtg ccaggcggtt gaagagcttg tcctcgtagt gcgggatgtc
      841 cttccaccag aggaagaccg gcacgtccgg cacgagcagc ggcgagaccg ccgtcgagag
      901 cgtctgcacg atcggcccgc ccgcctcgat ggtgacctgc tcgccacaga tgcgcttcgc
      961 cccgcggctc gcgagctggc accgcgtcga gacgtaggct tcgagccgcg cctcctcgcc
     1021 cgcgcgatcc gcatagagca cgatcgagcg gcacgggtgg cgctcgacca cctcttccag
     1081 gagcgcgtcc acctccgcgc gctcatcctg ccgctccgag tagacgatca gattcagcgc
     1141 gcacgcgcgc gtcacgcccg ccgacggcga cgacgacccg gcctcctgcc cgcccgcgcc
     1201 catctcggcc cacatcgccg ccagctccct ttcgagcttc acggcgtcca cgcccttcgc
     1261 cgccgacagg gcgaccgcgc tcgattgtgt ctccatagga aaaccgtaaa ccgtgaatcg
     1321 taaaccgtga acggtgaaga gccttgtcgg gcaaaccgct gaccgtgagg agcgcagaac
     1381 acttgtcacg gttcacggtt cacggctctt agggtctgcg ccagcggcgc ccgttctgtt
     1441 cgaggatgcg ggaggcttct tccggccccc acgagccggc ctcgtagttg gggaaggtgg
     1501 ccgtctcttt gtgctccccc cattcttcga gtatcggctc gacgatctcc cagccgcgct
     1561 cggtcatgtc ggcgcgcgcg tagagggtgg agtcgccgag gatgcagtcc aggatcaaac
     1621 gctcgtaggc ttcggcgagc tggacgccga acgaggcgcc gtagcggaag tccatcgtca
     1681 cgtcgcggat gcgcgtcacc tgccccggca ccttcgcggc gaagcgcatc gtgatgccct
     1741 cgtcgggctg gatgcggatg acgagctggt tcggctcgac gaactccatc gcccccgtgc
     1801 cgaagagctg gtgcggcgcc tgtttgaact ggatggcgat ctccgtgacg cgcttcgcca
     1861 gacgcttgcc cgagcggatg tagaacggca cgcccgacca ccgccagttg tcgaaccaca
     1921 tcgtgatcat ggcgtaggtc tcggtgaccg agtcgggcgc cacaccctgc tcctcgcggt
     1981 agccgggcac gggcttgccg ccgatcgagc cggggccgta ctgcccgcgc acgcactcgg
     2041 cacggacgcg ctcgggcgag aaggcacgcg ccgagtgcat ggccttgatc ttctcgtcgc
     2101 gcacgtcttc ggggccgagc gtcgcgggcg gctccatcgc cacgagcgac aagacctgaa
     2161 agacgtggtt ctggatcatg tcgcgcacca cgccggattt ttcgtagtag ccgccgcgcc
     2221 cctcgacgcc cacggtctcg gcgttcgtga tctgcacgtg gtcgatgtac tgccggttcc
     2281 acatcggctc gaagatgccg ttggcgaaac gaaagacgag gaggttctgg acggtctctt
     2341 tgccgaggta gtggtcgatg cggtaaatct gcttctcttc gaggtgcttc aggatttgct
     2401 ggttcagctc gcgcgcgctg tcgaggctcg tcccgaaagg cttctcgatg atgacgcgca
     2461 cccagccctt gccctgcttc gtcaaaccgg cttcgccgag ttgacgcaca gcctcggcgt
     2521 agaactcggg cgcgaccgaa aggtagaaga gacggttgcc ctgcgtcccg cgctcgcggt
     2581 ctacctgttc gagcatctcg gagagcacct gatagcactc cggcttctcg atgttcgacg
     2641 gcaggaacct gatcccctgc gcgaacgact gccacacctc ctcgtccacg cgcttggcct
     2701 cggagaattc gatcaccgac gccttcatct tctcgcggaa ctcgtcgtcc gacatcgccg
     2761 tgcggcccag accgatgatg gcgaactccg ccgggagcag gcgctcctgc acgagccgat
     2821 acagcgccgg gatgagcttg cgcttggcca gatcgcccga ggccccgaag atcaccaccg
     2881 cgcacggctc ggcggtgcgc tccagccgca cgccctcccg caaaggattg tccaaagtgc
     2941 tcgctgcttg catcgtcaaa cctcagtagg cagtaagcag taggcagtag gcagtagaac
     3001 tttttttgag gcggtcaggg gtaagcggta
//
LOCUS       DBNGOH010000521         4397 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4109285, whole genome shotgun sequence.
ACCESSION   DBNGOH010000521 DBNGOH010000000
VERSION     DBNGOH010000521.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4397)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4397)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4397
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4109285"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..768)
                     /locus_tag="AC159P_10165"
     CDS             complement(<1..768)
                     /locus_tag="AC159P_10165"
                     /inference="COORDINATES: protein motif:HMM:NF024753.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB
                     family protein"
                     /protein_id="HIR3695856.1"
                     /translation="MNRTTARLASHILLAFVLCGGGVAAQEWAQWRGPARDGVVKGFA
                     APKVWPKALVQKWKVAVGGGYSSPVVAGGRVYLHTRQGEQEVVSAFELATGKAVWTKS
                     YDASFEKNQYAVKMGKGPNSTPLVHAGRLYTFGVNAVLSAFDAKTGALVWRKDFGRPD
                     TSKLFCGTAMSPVIEKGLLVVHVGDDRGGKVVAFDAASGTERWSWAGDGPGYASPVVA
                     ELAGARQVVTLTDKSVVGLAADTGQLLWKMAFPDEWNE"
     gene            complement(926..2014)
                     /locus_tag="AC159P_10170"
     CDS             complement(926..2014)
                     /locus_tag="AC159P_10170"
                     /EC_number="6.2.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase family protein"
                     /protein_id="HIR3695857.1"
                     /translation="MASSETTLQSNVKASREALDVWVREVVEWHFDPETGSPFWLERA
                     QGLGFDPRREVRTYEDLGRFGPFEDEWLRGGPVRRWVPKGLAHKPVYVFETGGSTGVP
                     KSRISIEDFRIDYEMFSRTLPDEFFPPGSDWLMLGPSGPRRLRLAVEHLAQHRGGICF
                     TVDLDPRWVIKLLKSGQPRQAEDYKRHVVEQGLTVLRAHPNVKCLFTTPKLLEALCEK
                     ISLKKAGITGVFCGGTEMTPQFHRFAVEELLEGAYFAATYGNTLMGLAVHKPRAEGDP
                     FDIIYYPPAPRAMIEVVDPDQPDRVVGYGETGRVRLTTLTKEFFMPRFLERDEAEREP
                     PCDPYPWDGVRNVRPFSRLQTTVVEGVY"
     gene            complement(2295..3089)
                     /locus_tag="AC159P_10175"
     CDS             complement(2295..3089)
                     /locus_tag="AC159P_10175"
                     /inference="COORDINATES: protein motif:HMM:NF024743.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HIR3695858.1"
                     /translation="MRKQLFRIASVLCLCVASAAVASAQDFQKSYRLGADGSVSIRNV
                     SGAINITGYDGDAVTVSGFKEGRDRERVEVVDESTAGAVNISVEYPRNCDCDASIRFE
                     VRVPRSVAYDFSRIATASGDISFKGVRGRVRARTASGEVLVEDVTGDIDASTASGNVS
                     VREAAGTVNASSASGDVDVEIARLEGTGNMKFSTASGDVSVRLPSSLDADVSLSSMSG
                     KVQTSFPIEVKERTHGPGSYAEGRLGGGARTLRISSASGNVSLTGK"
     gene            3368..4279
                     /gene="xerD"
                     /locus_tag="AC159P_10180"
     CDS             3368..4279
                     /gene="xerD"
                     /locus_tag="AC159P_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HIR3695859.1"
                     /translation="MARDLIREYLSFIQVEKGLSANSLEGYRHDLGKLRRWAAQERGR
                     EPHELDKKDLAQWVMSLSRGGMGARSVARAISAARGYYRFLLLDGHTHADPTAQLVAP
                     APGQKLPRFLTKEEMSKLLEAPDTATVEGTRDRAMIELLYATGLRVTELVSLKLGEID
                     LDGGLLSCTGKGSKQRKVPVGRSALSWVEKYLGLRRKLLGESESARLFVRAGAQTLTR
                     QHVWALLKTYAARAGLDGVSPHVLRHSFATHLLEHGADTRSVQAMLGHSDLATTQIYT
                     HVTGERLRAAYERHHPRARRGAGGADE"
BASE COUNT          693 a         1554 c         1416 g          734 t
ORIGIN      
        1 ctcgttccac tcgtctggga aggccatctt ccagaggagc tggcccgtgt cggcggccag
       61 ccccacgacc gacttgtccg tcagcgtgac gacctgccgc gcgcccgcca gctccgcgac
      121 cacgggcgag gcgtagccgg ggccgtcgcc cgcccaagac caacgctccg tgcccgaggc
      181 ggcgtcgaac gcgacgacct tgccgccgcg gtcgtcgccg acgtggacga cgagcagccc
      241 cttctcgatc accggcgaca tggccgtgcc gcagaagagc ttcgacgtgt cgggccgccc
      301 gaagtctttg cgccacacga gcgcgcccgt cttcgcgtcg aacgcggaga ggacggcgtt
      361 gacgccgaac gtgtagaggc gccccgcgtg gacgagcggc gtcgagttcg gccccttgcc
      421 catcttgacg gcgtactggt tcttctcgaa cgaggcgtcg taagacttcg tccacacggc
      481 cttgcccgtc gccagctcga acgccgagac gacctcctgc tcgccctgcc gcgtgtgcag
      541 gtacacccgc ccgcccgcga cgaccggcga agagtagccg ccgccgaccg ccaccttcca
      601 cttctgcacg agcgccttcg gccacacctt cggcgccgcg aaccccttca ccacgccgtc
      661 gcgcgcaggg ccgcgccact gcgcccactc ctgcgccgcg acgccgccgc cgcacaagac
      721 gaacgccaac aggatgtgcg atgcgagccg tgctgtcgtg cggttcattg tcggtgagat
      781 ctttccagga cgacattcaa cgtttgaaat atctgtaggg gcagggcttg tccctgcccg
      841 cagtcacgga ttcattcagc attgaatcga tcggaggctg acgggccgcg gacttgccct
      901 gcccctacag ttcattcggt cgccctcagt acacgccctc gaccaccgtt gtctgaaggc
      961 gcgagaaggg gcggacgttg cgcacgccgt cccacggata tgggtcgcag ggcggctcgc
     1021 gctcggcctc gtcgcgctcc agaaagcggg gcatgaagaa ctctttggtg agcgtcgtca
     1081 ggcggactcg tccggtctcg ccgtagccga cgacgcggtc gggctggtcg gggtcaacca
     1141 cctcgatcat cgcgcgcggc gcgggcgggt agtagatgat gtcgaagggg tcgccttcag
     1201 cgcggggctt gtgcaccgcg aggcccatca gcgtgttgcc gtaggtcgcg gcgaagtagg
     1261 cgccttccaa tagctcttcg acggcgaagc ggtggaactg cggtgtcatc tccgtgccgc
     1321 cgcagaagac gccggtgatc cccgccttct tcagcgagat tttttcgcac aacgcttcca
     1381 ggagcttcgg cgtcgtgaac aggcacttga cgttcgggtg cgcgcgcagc accgtcaggc
     1441 cctgctcgac gacgtgccgc ttgtagtctt cggcctgtcg aggctgtcca gacttgagga
     1501 gcttgatgac ccagcgcggg tctagatcga cggtgaagca gatgccgccg cggtgctgcg
     1561 cgaggtgctc gacggcgagc ctgagcctgc gcggccccga agggccgagc atcagccaat
     1621 ccgagccggg cgggaagaac tcgtccggca gcgtgcggct gaacatctcg tagtcgatgc
     1681 ggaagtcttc gatgctgatg cgcgacttcg ggacgcccgt ggagccgccc gtctcgaaga
     1741 cgtagaccgg cttgtgcgcc agccccttcg gcacccagcg gcggacgggg ccgccgcgca
     1801 gccactcgtc ctcgaacggg ccgaagcgcc cgaggtcttc gtaagtccgc acctcccggc
     1861 gcgggtcgaa gcccaggccc tgcgcgcgtt cgagccagaa ggggctgccc gtctccgggt
     1921 cgaagtgcca ctccacgacc tcgcgcaccc acacgtccag cgcctcgcgc gaagccttga
     1981 cgttactctg tagtgtcgtt tcgctgctcg ccatcttgcg acgcagatta ctaattcccg
     2041 cacgccgcgc acaacccccc ggcggcgaaa aagccgcgcc gtgtttgtaa aatgttattt
     2101 acatccccgg cgaccaaggc gcgggagcaa gaaggagggc cgcatgtcgt cgtcgtggac
     2161 gcgcggcccg acgttgcgct catggttcaa gaagtacggc ggtcacgcct cgaagccgaa
     2221 gcgtgaccgc cgtacgattt gtttccgacg atgatgtcgg acgactcaag aagtcttgag
     2281 ccgccccgcc gcgctcactt cccggtcagg ctcacgttgc ccgaggcgga cgagatgcgg
     2341 agggtgcgcg cgccgccgcc gaggcgccct tcggcgtagc tgccggggcc gtgggtgcgc
     2401 tccttcacct cgatggggaa gctggtctga accttgccgc tcatgctcga caggctcacg
     2461 tcggcgtcga ggctcgaagg cagacgcacg ctcacgtcgc cgctcgcggt cgagaacttc
     2521 atgttgcccg tgccttcgag gcgcgcgatc tccacgtcca cgtcgccgct cgcgctcgac
     2581 gcgttgaccg tgcccgcggc ctcgcgcacg ctcacgttgc cgctcgccgt cgaggcgtcg
     2641 atgtcgcccg tcacgtcctc gaccagcacc tcgccgctcg ccgtgcgcgc gcgcacgcgg
     2701 ccccgcacgc ccttgaaact gatgtcgccg ctggcggtcg cgattcgcga gaagtcgtac
     2761 gcgaccgagc gcggcacgcg cacctcgaag cggatgctgg cgtcgcaatc gcaattgcgc
     2821 gggtactcca cgctgatgtt gacggcgccc gcggtgctct cgtccacgac ctcgacccgt
     2881 tcgcggtcgc ggccttcctt gaagccgctg acggtcacgg cgtcgccgtc gtagcccgtg
     2941 atgttgatgg cgcccgacac gttgcggatg ctgaccgagc cgtccgcgcc gaggcggtac
     3001 gacttctgga agtcctgcgc cgaggcgacc gccgccgacg cgacgcacag gcacaacacg
     3061 gacgcgattc tgaacagttg ctttctcatg gctgatggct gctccttgca gatggaagtg
     3121 tctctgacgt tcgcgggcac tctcgcgctc gcgccccctc gtcgctctgt tgtggaaaca
     3181 ccggcgcgcg ggaattcgtg gcagcaaatt tccaagcaag cgggcagcag gcagcaagct
     3241 gcaggcagtc gatacaaaaa cagatccgag atttcaagtt cggtaagcct ttactgcctg
     3301 ctgcccgctg cctgctgccc gcttcttttt cttgtcggct caatgctttt gtgtttaatc
     3361 tcctcgcatg gcacgtgacc tgatccgcga atacctctct ttcatacagg tcgagaaggg
     3421 gctgtcggcc aattcgctcg aaggctaccg gcacgacctc gggaagctgc ggcggtgggc
     3481 ggcgcaggag cgcgggcgcg agccgcacga gctggacaag aaagacctcg cgcagtgggt
     3541 gatgtcgctc tcgcgcggcg ggatgggggc gcgctcggtg gcgcgcgcca tcagcgccgc
     3601 gcgcggctac taccgcttcc tgctgctcga cggccacacc cacgccgacc cgaccgcgca
     3661 gctcgtcgcc cccgcgccgg ggcagaagct cccgcgcttc ctcacgaagg aggagatgag
     3721 caagctgctc gaagcgcccg acaccgcgac cgtcgaaggc acgcgcgacc gcgcgatgat
     3781 cgagctgctc tacgccacgg gcctgcgcgt caccgaactc gtctcgctga agctcggcga
     3841 gatcgacctc gacggggggc ttctgagctg caccggcaag gggagcaagc agcgcaaagt
     3901 ccccgtgggc cgcagcgcgc tctcttgggt cgagaagtac ctcggcctcc ggcgcaagct
     3961 cctcggcgaa agcgagagcg cgcgcctctt cgtccgcgcg ggcgcgcaga cgctcacgcg
     4021 ccagcacgtc tgggcgctgc tcaaaactta cgccgcgcgt gccggcctcg acggcgtctc
     4081 gccccacgtc ctgcgccaca gcttcgccac gcaccttttg gaacacggcg ccgacacgcg
     4141 ctccgtccag gccatgctcg gccacagcga cctcgccacc acgcaaatct acacgcacgt
     4201 caccggcgag cgcctgcgcg ccgcctacga gcgccaccac ccgcgcgcgc gccggggcgc
     4261 gggcggggcg gacgagtaga cgggcctaac cgctttcgct tggtcattcg actatggacg
     4321 accaccggcc agagttaaaa cgggcgctta cctggctgtg cctcttcctg cccctttgcg
     4381 cttcgtgtaa cgttccc
//
LOCUS       DBNGOH010000522         4001 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4475886, whole genome shotgun sequence.
ACCESSION   DBNGOH010000522 DBNGOH010000000
VERSION     DBNGOH010000522.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4001)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4001)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4001
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4475886"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            93..1532
                     /locus_tag="AC159P_10185"
     CDS             93..1532
                     /locus_tag="AC159P_10185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695860.1"
                     /translation="MTHLELIAGAAARERVRWAREHLYSLLILSPLVLGLTYFGVGRM
                     VSTNEDIVLTPQATAALAFVTAAGLILLSMSRASAEVYHLRSPETLFDTLAVAADTHL
                     YAALVERIARTSAVGAAAVVARTVFGGTLADAWLAPPLIVFVLLVALAEVLAALVWVH
                     WSRVRMAWQALAALAALAACAFVAGLLLVAVVKPERLTEGRRAAACVAGLILTGVLFG
                     VVRALHGSWRGADIEHAKHLGAGRRGGLGWVARARGFFKEAEPAALFERDLRLTLRAF
                     SSAVYASFALAVLVVAALAAALTSGAVPAGDAPPGVFDMTWLPRVVAVKVACVLAAVA
                     LASLVPVLVAHQAPHLWLERAVGAKGADVWRAKLWYARAVSLPAPIAAWAVGVLGGAS
                     PVAYVVPLLAEVLWLWWMVGTIAGALAYEVPEQPGLGVVMVACAGLAAGLTSAALWPL
                     GLALYGFGVGKMCLWGEHRARFYLLSEVK"
     gene            1529..2260
                     /locus_tag="AC159P_10190"
     CDS             1529..2260
                     /locus_tag="AC159P_10190"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3695861.1"
                     /translation="MSARAQEQLVVAEGLTKRYGEVLAVDGLSLSVAAGEVYALVGAN
                     GAGKSTTFRMLTGLSLPDAGAVNICGEDMRRHSARAKHHLGYLPEELYFYQRLTGREY
                     LRLVAGLKGADEEGVGCEIEFFELKAVEHKWVGGYSLGMRKKLGLAAAFAGRPRVLVL
                     DEPLNGLDVEMMRKLRLRLEEVSAEGRAVIVSSHVMSFVERVSARVGVMRAGRLVAEG
                     TPAELREAARLPDAPFEDVFFRLAK"
     gene            2348..>4001
                     /locus_tag="AC159P_10195"
     CDS             2348..>4001
                     /locus_tag="AC159P_10195"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013939362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3695862.1"
                     /translation="MSLPSEGESGHTPPADSATARTDGAQAPAGRADAAPPRRPDSDA
                     PADEERLRLILDAIPVPIAYVDAEQRYRFNNRAYDGWLGRPRRELYGKTVREMLGESA
                     YEEIRPHIEEALAGRESKYETELTYPDGSRRFISVTYAPDFDAAGRVRGFTATVSDLT
                     QRRRTEAALVEAEHRAVREYESLLTRLTQLAETLGTARDHLAVFRDLKNFAFASVPCI
                     GIFISLYDPERDVRLARYAFGDETEVDVSTLPPMPVSSEGPNSRAVRTGQVVITNDYW
                     RTKQQGKGQIGVLVGPDNGLRPQSSLVVPMQTMGRVVGTIEVQSYENEAYRAEHVTAM
                     RMAANLAAVAIENMRLLEHESAARAAAEESNRLKDEFLATLSHELRTPLTSILGWSNM
                     MLTGKLDDATARGAAESIERNARAQHQIVNDLLDVSRIITGQLRYEAQPTDVRAVVRA
                     AIETVRPAADAKNIRLQARFDPRADPVMGDPARLQQIAWNLLANAVKFTPAGGEVRVS
                     VERAGAHVRFAVSDTGEGVRADFLPFVFDRFRQADQSPTRRYG"
BASE COUNT          553 a         1319 c         1508 g          621 t
ORIGIN      
        1 gagagagcga tttgtcttct ccgtgaactc tgtgaaccct ctgtgccctc tgtgttaaat
       61 tgagcttcgg tcggactcaa gccgagaaca caatgacgca tctcgaactc atcgccggcg
      121 cggcggcgcg cgagcgtgtg cgctgggcgc gcgagcacct gtactcgctg ctcatcctgt
      181 cgccgctcgt gctcgggttg acctacttcg gcgtcgggag gatggtgagc acgaacgagg
      241 atatcgtgtt gacgccgcag gctaccgcgg cgctcgcgtt cgtgacggcg gcggggctga
      301 tcctgctcag catgtcgcgc gcgagcgccg aggtctatca cctgcgcagc cccgagacgc
      361 tattcgacac gctcgcggtg gcggcggaca cgcacctcta cgcggcgctt gtggagagaa
      421 tcgcgcgcac gtcggcggtg ggcgcggcgg cggtcgtggc gcggacggtc ttcggcggga
      481 cgctggcgga cgcgtggctg gcgccgccgt tgattgtgtt cgtcctgttg gtcgcgctcg
      541 cggaggtgtt ggcggcgctc gtgtgggttc attggtcgcg tgtgcggatg gcgtggcagg
      601 cgttggcggc gttggcggcg ctcgcggcgt gcgcgttcgt cgccgggttg ttgctcgtcg
      661 cggtggtgaa gccggagagg ctgaccgaag ggagacgcgc ggcggcgtgc gtcgcggggt
      721 tgatcttgac aggggtgctg ttcggagtcg tccgcgcgtt gcacgggagt tggcgcgggg
      781 cggacatcga gcacgcgaag catctgggcg cggggcgccg gggcgggctg ggatgggtcg
      841 cgcgcgcgcg cggatttttc aaggaggcgg agccggcggc gttgttcgag cgcgatctga
      901 ggttgacgtt gagggctttc tcttcggcgg tctacgcgtc gttcgcgctc gcggtgttgg
      961 tggtggcggc gctggcggcg gcgttgacga gcggcgcggt gcccgcgggc gacgcgccgc
     1021 cgggcgtgtt cgacatgacg tggctgccgc gcgtggtggc ggtgaaggtg gcatgcgtgc
     1081 tcgcggcggt cgcgctggct tcgctcgtgc cggtgctggt cgcgcaccag gcgccgcacc
     1141 tgtggctgga gcgcgcggtc ggcgcgaagg gcgcggacgt gtggcgcgcg aagctgtggt
     1201 acgcgcgcgc ggtgtcgctg cccgcgccga tagccgcgtg ggcggtcggc gtcctcggcg
     1261 gcgcgtcgcc ggtggcgtac gtcgtgccgc tgctcgcgga agttctgtgg ctgtggtgga
     1321 tggtcgggac gattgcgggc gcgctcgcgt acgaggtgcc ggagcagccg ggcctcggcg
     1381 tcgtgatggt ggcgtgcgcg gggctggcgg cggggctgac gtccgccgcg ctctggccgt
     1441 tggggctggc gctgtacggg ttcggcgtcg ggaagatgtg tctgtggggc gagcaccgcg
     1501 cgcgcttcta cctgctctcg gaggtgaagt gagcgcgcgc gcgcaggagc aactggtcgt
     1561 cgccgagggt ttgacgaagc gttacggcga ggttctggcg gtggacggct tgagcctgag
     1621 cgtcgcggcg ggcgaggtct acgcgctggt cggcgcgaac ggcgcgggca agtccacgac
     1681 gttccgcatg ctcacggggt tgagtctgcc ggacgcgggg gccgtgaaca tctgcggcga
     1741 ggacatgcgg cggcattcgg cgcgcgccaa gcaccacctc ggctacctgc ccgaggagct
     1801 ttacttttac caacggctga cggggcgcga gtacctgcgg ctggtcgcgg ggctgaaggg
     1861 cgccgacgag gaaggcgtcg gctgcgagat cgagttcttc gagctgaagg cggtcgagca
     1921 caagtgggtc ggcggctact cgctcgggat gcggaagaag ctggggctgg cggcggcctt
     1981 cgccggtcgc ccgcgcgtgc tcgtgctgga cgagcctttg aacgggttgg acgttgagat
     2041 gatgaggaag ctgcgattgc ggctcgaaga agtgagcgcc gaagggcgcg cggtcatcgt
     2101 ctcttcgcac gtgatgagct tcgtcgagcg cgtgagcgcg cgcgtcggcg tgatgcgcgc
     2161 gggccgcctc gtcgccgaag gcacgcccgc cgaattgcgc gaggcggcac ggctgcccga
     2221 cgccccgttc gaggacgttt tcttccggct ggcaaagtga atccggaaag acgataataa
     2281 cccgccgcgc cggcccgcgc gcgcgacctc gctccccgaa ttggccgaac cccgggttat
     2341 aagaaagatg tctctgccca gcgaaggcga gagcggccac acgcctcccg ccgattcagc
     2401 aactgcccgc acggacggcg cgcaagcgcc cgcgggccga gccgacgcgg ccccgccccg
     2461 ccgccccgac tcggacgcgc ccgccgacga agagcgtctg cgcctcatcc tcgacgccat
     2521 ccccgtcccc atcgcctacg tcgatgccga acagcgatac cgcttcaaca accgcgccta
     2581 cgacggctgg ctcgggcgcc cgcgccgcga gctgtacggc aagaccgtgc gcgagatgct
     2641 cggcgagagc gcctacgaag agattcgacc gcacatcgag gaggcgctcg cggggcgcga
     2701 gtcgaagtac gagaccgagc tgacctaccc ggacggctcg cgccgcttca tcagcgtcac
     2761 ctacgcgccg gacttcgacg ccgccgggcg cgtgcgcggc ttcaccgcca cggtcagcga
     2821 cctcacgcag cggcgccgca cagaggcggc gctggtcgag gccgagcacc gcgcggtgcg
     2881 cgagtacgag tcgctgctca cgcgcctgac gcagctcgcc gaaacgctcg gcacggcgcg
     2941 cgaccacctc gccgtcttcc gcgacctgaa aaacttcgcc ttcgcgtccg tcccgtgcat
     3001 cggcatcttc atctcgctct acgacccgga gcgcgacgtg cgcctcgcgc gctacgcctt
     3061 cggcgacgag accgaggtgg acgtgagcac gctgccgccg atgcccgtct cctccgaagg
     3121 gccgaacagc cgcgccgtgc gcacgggcca agtcgtcatc acgaacgact actggcggac
     3181 gaagcagcag gggaaggggc agatcggcgt gctggtcggg ccggacaacg gcctgcgccc
     3241 gcagtcttcg ctcgtcgtcc cgatgcagac gatggggcgc gtcgtcggca ccatcgaggt
     3301 gcagtcgtac gagaacgaag cctaccgcgc cgagcacgtg acggcgatgc ggatggcggc
     3361 caacctcgcg gcggtggcca tcgagaacat gcggctgctc gaacacgaga gcgccgcgcg
     3421 cgccgccgcc gaagagtcga accgcctgaa ggacgagttc ctcgccacgc tctcgcacga
     3481 gctgcggacg ccgctgacct cgatcctcgg ctggtcgaac atgatgctca cgggcaagct
     3541 cgacgacgcg accgcgcgcg gcgccgccga gagcatcgag cgcaacgcgc gcgcacagca
     3601 ccagatcgtc aacgacctct tggacgtgtc gcgcatcatc acgggccaac tccgctacga
     3661 ggcgcagccg accgacgtgc gcgccgtcgt gcgcgccgcc atcgagaccg tccgccccgc
     3721 ggcggacgcg aagaacatcc gtctgcaagc ccgcttcgac ccgcgcgccg accccgtcat
     3781 gggcgacccc gcgcggctgc aacagatcgc gtggaacctg ctggcgaacg ccgtcaagtt
     3841 cacgcccgcg ggcggcgagg tgcgcgtctc ggtggagcgc gcgggcgcgc acgtccgctt
     3901 cgccgtctcc gacacgggcg agggcgtccg cgcggacttc ctccccttcg tcttcgaccg
     3961 cttccggcag gccgaccagt cgccgacgcg ccgctacggc g
//
LOCUS       DBNGOH010000523         2385 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3193100, whole genome shotgun sequence.
ACCESSION   DBNGOH010000523 DBNGOH010000000
VERSION     DBNGOH010000523.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2385)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2385)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2385
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3193100"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(171..1751)
                     /locus_tag="AC159P_10200"
     CDS             complement(171..1751)
                     /locus_tag="AC159P_10200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695863.1"
                     /translation="MSEFSRREFLLAGALAALAGRGALAQSPLKIPRPGRELEVTLLG
                     RADDSKEEVVSFGVPLPPDFLSDAGRLRVLDERGEEVAATTRALEPWRLGGREGGSVR
                     SLLVQFKSDFRTAHERRVTLLFDKSPKRRGGPLVPVRETLIDGEGLKGPRVLAVLPAK
                     WLCDSWVVGPQTPASESGEYAPYEQSVERHFEGSLKHLDSREFAAWLYDRPSNYYKMY
                     ARTGDRKFLEAGYRAAHFMRLNTRMDGPDAGIFKLKGADIKYVYPRAMHLHYLLTGDE
                     RALETGRLMARYCLTHTDPVYRPARMTPSSPDKDPEKGREFWTLRNQGYGLLGVLHGW
                     EMTGERAYWEKARECVEAYDKHQRQPPDGLPADGSLRQNWALYDSGEATFEGATSAWM
                     MALLLDPLFMYWTMTGDRRVPEMVLKWCDFLDRRGMVPDGSKAYYVINCFAKPGEPGG
                     ELGPDMEMHNPEMAYTFALGIYFAKEPARRAVYRRRFERLFPLAVKLADVNKPPRAFS
                     WVFQFTSQLVYFMQRAGS"
     gene            complement(1765..>2385)
                     /locus_tag="AC159P_10205"
     CDS             complement(1765..>2385)
                     /locus_tag="AC159P_10205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR3695864.1"
                     /translation="RFDPMWEAAGGARLPVAIHTSDPEAFFLPTDKYNERYEELSAHP
                     DWSFHGHDFPSNRELHEARNRVVARHPRTHFVYLHVGNAEDLAWVSEWLDRYKNTSVE
                     IGARIGELGRQPRAARKFFDKYQDRSLFGTDAVPDGFETPQQIFGDELYEIYYRFLET
                     EDEYFDYAPAPKPPQGRWQIYGLGLPDEILRKVYWENAARLLGLKG"
BASE COUNT          366 a          802 c          824 g          393 t
ORIGIN      
        1 tggccgttga agcgctggaa cgtctggatg cccatccccg gattgacgag cacctcgtcg
       61 ctctcggtgg gccggacgac gaccgtctcg ccctgcgcgt cggcgacgcg gcagagtccg
      121 gccgagagga gcaacaggag cgcgactctc ttcagcaaca atcccatccc tcaactcccg
      181 gcgcgctgca tgaagtagac gagctggctc gtgaactgga agacccagct gaaggcgcgc
      241 gggggcttgt tgacgtcggc gagcttgacg gccagcggga agagccgttc gaagcgccgg
      301 cgatacacgg cgcggcgcgc gggctctttg gcgaagtaga tgcccagggc gaaggtgtag
      361 gccatctcgg ggttgtgcat ctccatgtcc ggccccaact cgccgcccgg ctcgcccggc
      421 ttcgcgaagc agttgatgac gtagtaagcc ttcgacccgt ccggcaccat cccgcggcgg
      481 tcgaggaagt cgcaccactt caagaccatc tcgggcacgc gccggtcgcc cgtcatcgtc
      541 caatacatga agagcgggtc caagagcagc gccatcatcc aggccgacgt ggcgccctcg
      601 aacgtggcct cgcccgaatc gtagagcgcc cagttctggc ggagtgagcc gtctgccggc
      661 agtccgtcgg gcggctgccg ctggtgcttg tcgtacgcct cgacgcactc gcgcgccttc
      721 tcccagtagg cgcgctcgcc cgtcatctcc cagccgtgga gcacgcccag caacccgtag
      781 ccctggttgc ggagcgtcca gaactcccgg cccttctccg ggtctttgtc gggcgaactc
      841 ggcgtcatgc gcgccgggcg gtagacgggg tctgtgtgcg tgaggcagta gcgcgccatg
      901 aggcggcccg tttcgagcgc gcgctcgtcg ccggtgagga ggtagtgcag atgcatcgcg
      961 cgtgggtaga cgtacttgat gtccgcaccc ttcagcttga agatgcccgc gtcggggccg
     1021 tccatccgtg tgttgagccg catgaagtgc gcggcgcggt agccggcttc gaggaacttc
     1081 cggtcgccgg tgcgcgcgta catcttgtag tagttggacg ggcggtcgta gagccacgcg
     1141 gcgaactcgc ggctgtcgag gtgcttgagc gacccctcga agtggcgctc gaccgactgt
     1201 tcgtaagggg cgtattcacc cgactcggag gcgggcgtct gcgggccgac gacccacgag
     1261 tcgcacagcc acttcgcggg cagcacggcg aggacgcgcg gccccttcag cccttcgccg
     1321 tcgatgagcg tctcgcgcac ggggacgagc gggccgccgc gcctcttggg cgacttatcg
     1381 aagagcaggg tgacacgcct ctcgtgcgcg gttcggaagt cggacttgaa ctggacgagg
     1441 agcgagcgga cggaacctcc ctctcgcccg ccgagccgcc acggttcgag agcgcgcgtc
     1501 gtcgccgcga cctcctcgcc gcgctcgtcg agcacgcgca agcgccccgc gtccgagaga
     1561 aagtcgggcg ggagcgggac gccgaagctg acgacctctt ccttcgaatc gtccgcccgg
     1621 cccaggagcg tcacctccag ctcgcgcccc ggtcttggaa tcttcagcgg cgactgcgcg
     1681 agcgccccgc gccccgcgag cgcggccaac gccccggcca gcaggaactc tctccgcgaa
     1741 aactccgaca tccttagctc cccttcagcc cttcagcccc agcaggcgcg cggcgttctc
     1801 ccagtagact ttgcgcagaa tctcgtcggg cagccccagc ccgtaaatct gccagcgccc
     1861 ctgcgggggt ttgggcgccg gggcgtagtc gaagtactcg tcctcggtct caaggaagcg
     1921 gtagtagatt tcgtacagct cgtcgccgaa gatctgctgc ggcgtctcga agccgtcggg
     1981 cacggcgtcg gtgccgaaga gactcctgtc ctgatacttg tcgaagaact tacgcgcggc
     2041 gcgcggctgg cggcccagct cgccgatgcg cgcgcctatc tcgaccgagg tgttcttgta
     2101 gcggtcgagc cactccgaga cccacgcgag gtcttcggcg ttgccgacgt gcaggtagac
     2161 gaagtgcgtg cgcgggtggc gcgcgacgac gcggttgcgc gcctcgtgca gctcgcggtt
     2221 cgaggggaag tcgtggccgt ggaaagacca gtcggggtgc gcgctcagct cctcgtagcg
     2281 ctcgttgtac ttgtcggtcg ggaggaagaa cgcctcgggg tcggaggtgt ggatggcgac
     2341 ggggaggcgc gcgccgcccg ctgcctccca catcgggtcg aagcg
//
LOCUS       DBNGOH010000524         6701 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2826734, whole genome shotgun sequence.
ACCESSION   DBNGOH010000524 DBNGOH010000000
VERSION     DBNGOH010000524.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6701)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6701)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6701
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2826734"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..296
                     /locus_tag="AC159P_10210"
     CDS             <1..296
                     /locus_tag="AC159P_10210"
                     /inference="COORDINATES: protein motif:HMM:NF012296.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HIR3695865.1"
                     /translation="YHIHRHPAFWAEPETFDPDRWLAGSGSRGHQLSFMPFGAGQRLC
                     IGNHMALMEGALLLAQIAQRYEPRLASPDPALPDVGVTMTPKNGLPMLIRSRV"
     gene            complement(577..666)
                     /locus_tag="AC159P_10215"
     tRNA            complement(577..666)
                     /locus_tag="AC159P_10215"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(630..632),aa:Ser,seq:cga)
     gene            complement(727..1257)
                     /gene="tadA"
                     /locus_tag="AC159P_10220"
     CDS             complement(727..1257)
                     /gene="tadA"
                     /locus_tag="AC159P_10220"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017300505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HIR3695866.1"
                     /translation="MPAREEIGAGASADTADERRMRMAVEAAREAARRGEVPVGAVVV
                     GSDEVVLAVAGNRTRTDCDPTAHAEVVALREAARRAGNYRLTGATVYSTVEPCAMCAG
                     ALVQARVGRLVYGAVDVRAGAVESVFRVCDSSSLNHRMELTAGVLAGECRELMQEFFR
                     ARRQKENDECGMMNDE"
     gene            1492..2619
                     /locus_tag="AC159P_10225"
     CDS             1492..2619
                     /locus_tag="AC159P_10225"
                     /inference="COORDINATES: protein motif:HMM:NF021539.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2270 domain-containing protein"
                     /protein_id="HIR3695867.1"
                     /translation="MLLSNATDETTPLPPPAGNHIDDDHRTMKTDTRPRVSGPLAPLD
                     RAKPPKPQPAPPTEPETSSIHQTISPEQAAAASVAPPRVPRKFTPAEFNTAMVHFYRG
                     EVSRANTWRNRLDTTTNWAVLTTGATLSFAFSSPTNPHFVIVINSILVSFFLIMEARR
                     YRYYEIWSSRVRVLETGYFTQVLAPDLAPDEVWVEHLINDLKTPHFTISEWEAIGRRL
                     RRNYLWIFALLAAAWNLKVYLHPVPAYDFNAFIDRATVGIVPGEIVFVIGVIFNVGVF
                     IFAMATVRLREAVGEVLPEHNFHPIQTMTDWTRAATTKRQTATVRRAKKARERTRATT
                     PPRTGSVTGEWKRPDLARAQQQQSSDDIHRTQFPHKEPAMK"
     gene            2737..4236
                     /gene="hutH"
                     /locus_tag="AC159P_10230"
     CDS             2737..4236
                     /gene="hutH"
                     /locus_tag="AC159P_10230"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - L-histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HIR3695868.1"
                     /translation="MLELDGQQFSLEQVGAVARGAEGVRLAAAARGRMEASRRTVERI
                     IEEGRVVYGVNTGFGKLSDISIPPQELRELQINLVRSHAGGVGPALSEAETRAMMLLR
                     ANVLALGYSGARASVAETLLAMLERGVHPFIPEKGSVGASGDLAPLSHLALVCVGEGE
                     ARYQGETLAGGEAMRRAGIEPLTLEAKEGLALLNGTQALTAVGALALERAERLARVAD
                     VAGAMTLEALKGTPAAFDERIHAARPHKGQRETAARLRELLSESEIRASHLTGDPRVQ
                     DAYSLRCMPQVHGAVRDALAHARGATEIETGGATDNPLVFAETGDVISGGNFHGAPLA
                     LAFDYSAIALVSLGNISERRIERLVNPDMNEGLPAFLTSHAGVASGFMVAQIAAVALL
                     NECKVLAHPASADNLPTDGGKEDHVSMGMTAATKLRSIVENVEHVLAIELMCAAEGLD
                     YRAPLRPGRGVRRAHEAVRAHVPRLTRDRALSADFRKLAEAIRRGDFDL"
     gene            complement(4349..4729)
                     /locus_tag="AC159P_10235"
     CDS             complement(4349..4729)
                     /locus_tag="AC159P_10235"
                     /inference="COORDINATES: protein motif:HMM:NF016408.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HIR3695869.1"
                     /translation="MSSQAVARREEREHPTNTPKDNWLGFVGDVLMFTGEVRFKSMLR
                     IDGNFSGRVNSSDGTLVVGTGAQVTDAVIEVAVAKINGTVEGDISASQELVLGRTASV
                     KGKLAAHSLIIEEGAQFDGSCRWI"
     gene            complement(5245..5715)
                     /locus_tag="AC159P_10240"
     CDS             complement(5245..5715)
                     /locus_tag="AC159P_10240"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002617618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0047617 - fatty acyl-CoA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HIR3695870.1"
                     /translation="MSDLSLQEKYSPQGACFGCGPANEKGLHVRSFPSEDGEQIVAVW
                     TPAAVYEAFPGVLNGGIVGALLDCHSNWAAAYHFMKRDRLPRPPCTVTADYAVKLLRP
                     TPTDGPVALSARVVESNGDRATVEAELVAGGKLCATCRGTFVAVRPGHPAYHRW"
     gene            complement(5728..>6701)
                     /locus_tag="AC159P_10245"
     CDS             complement(5728..>6701)
                     /locus_tag="AC159P_10245"
                     /inference="COORDINATES: protein motif:HMM:NF023260.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3352 domain-containing protein"
                     /protein_id="HIR3695871.1"
                     /translation="GNEIAVSTPVDWFEGRRGFITDAAREAESSGEKPAKAGPVALVS
                     LNNPEAARKVVPLLLTGMGLPVQASAATERRKGFEVHDLGALAYAFVNNFLVVGESAP
                     AVRHVIDAYSSNQTLAASNRFRDAVAWQEPQRLALAYLSDGLMRDWIDGAKKMGDGSE
                     DPVVHAALAQLDVPLSAASYAATNEGDALLHELRLPVGLVKVFAASTVIGIKEAPVLG
                     NETFATYFLQRIVSAQQQFKDGKGKGRYGSLEELYEAKLLQTSEGSDGDYAYELHASG
                     DKFTATATPRNYGKTGRRSFYTDETGIVRAANHKGEPATAADPPIDR"
BASE COUNT         1082 a         2305 c         2246 g         1068 t
ORIGIN      
        1 tctatcacat ccaccgtcac cccgctttct gggccgagcc ggagacgttc gaccccgacc
       61 gctggctggc cggaagcgga agcagagggc accagctatc tttcatgccg ttcggggcgg
      121 ggcagagact ctgcatcggc aatcacatgg cgctcatgga gggcgcgctg ctgctcgcac
      181 agatcgccca acggtatgag ccgagactcg cctcgcccga cccggccctt cctgacgtgg
      241 gcgtcaccat gacgccgaag aacggattgc cgatgctgat tcgctcaagg gtatgaattc
      301 gagcacacct tcgccacgag gctcagggcg gcgaacgtac acgagtacga catcgcagac
      361 ctcctgggcc actcgaccac gccgggcgag acccgcaaca cgaaggtcac gcgcggctac
      421 gcccacggcg ttttgcaaag gctcagggac gccgtcaaca gcttggagga ggggaagcgc
      481 ctgatttcgg gtacgccgac tctacgccaa gtggcgcggg gcgcgtgagg ggcgaaaata
      541 ggtcagcccc ctaggtggct gaccctgaaa gagaaatggc ggagagggtg ggattcgaac
      601 ccacggtggc ctttcgacca ctccggtttt cgagaccggt gcactaaacc actatgcgac
      661 ctctccgcaa agggaaatga tgaatgatga gtgcggaatg atgaatgaaa ggcacgaccc
      721 tcttcatcat tcatcgttca tcattccgca ctcatcattt tctttttggc ggcgcgcccg
      781 gaagaattcc tgcatcagtt cgcggcactc tcccgcgagc acgcccgccg tcagttccat
      841 cctgtggttc aaagagcttg agtcgcagac gcggaagacg gattcgaccg cgcccgctct
      901 gacatcgacc gcgccgtaga cgagccgccc gacccgcgcc tgcacgagcg cgcccgcgca
      961 catcgcgcac ggctcgacgg tcgagtagac ggtggcgccc gtcagccggt agttgccggc
     1021 gcggcgggcg gcctcgcgca gcgcgaccac ttcggcgtgc gctgtcgggt cgcagtcggt
     1081 gcgcgtgcgg ttgcccgcga cggcgagcac cacttcgtca ctgccgacga ccacggcgcc
     1141 cacgggcacc tcgcccctgc gggcggcctc gcgcgccgcc tcgacggcca tgcgcatccg
     1201 gcgctcgtcg gctgtgtcgg cggacgcgcc cgccccgatt tcctctcgcg ccggcatggc
     1261 tctttcgacg ggtgctttca agaagcggcg gacaaaaatc gactataggg gcggcgcgcg
     1321 gcgcgtgtca agcagcccgc tttcgacggc ccgcggcccc gcgcggccag cgtaaaaccg
     1381 ggagacaacg cggcggcggg gtgacgcttc tcaattggtg gggcggcgcg ccgccgtcct
     1441 cgaccgattc ggcgcggcgc cttactttct ctccccccac gggagccgcg catgctatta
     1501 tcgaacgcga cagacgagac gacacccctc ccgccgcccg ccggaaacca cattgacgac
     1561 gaccaccgga cgatgaagac tgacacgcgc ccgagagtga gcggcccgct cgcgccactc
     1621 gacagagcca agccgccgaa gccgcagccg gcccccccga ccgaacccga aacgtcttcg
     1681 atccaccaga cgatctcgcc cgagcaggcc gccgccgcga gcgtcgcgcc gccgcgcgtc
     1741 ccgcgcaagt tcacgcccgc cgagttcaac acggcgatgg tgcacttcta ccgcggcgag
     1801 gtctcgcgcg ccaacacctg gcgcaatcgc ctcgacacga cgaccaactg ggccgtgctg
     1861 acgaccggcg ccacgctctc gttcgccttc tcgtcgccca cgaacccgca cttcgtcatc
     1921 gtcatcaact cgatcctcgt ctccttcttc ctcatcatgg aggcgcggcg ctaccgctac
     1981 tacgaaatct ggtcgagccg cgtgagggtg ctggagaccg gctacttcac gcaagtgctg
     2041 gcgcccgacc tcgcgcccga cgaggtgtgg gtcgagcacc tcatcaacga cctcaagacg
     2101 ccgcacttca ccatcagcga gtgggaggcc atcgggcggc gcctgcgccg caactacctc
     2161 tggattttcg cgctgctcgc cgccgcgtgg aacctcaagg tctacctgca cccggtgccg
     2221 gcctacgact tcaacgcctt catcgaccgc gcgaccgtcg gcatcgtgcc cggcgagatc
     2281 gtcttcgtca tcggcgtcat cttcaacgtc ggcgtcttca tcttcgcgat ggcgaccgtg
     2341 cgcctgcgcg aggcggtcgg cgaggtgctg cccgaacaca acttccaccc gatccagacc
     2401 atgaccgact ggacgcgcgc cgccaccacc aagcgccaga ccgccaccgt ccgccgtgcc
     2461 aagaaggcgc gcgagcgcac gcgcgccacc acgccgccgc gcaccggctc cgtcaccggc
     2521 gagtggaagc gccccgacct cgcccgcgcg caacaacaac aatcgtccga cgacatccac
     2581 cgcacacagt tcccgcacaa ggagccggcg atgaaatagt aggcagtagg cagcgggcag
     2641 ttaagaaagt gcgtatctcg aatttgaatt ttcgaattcc gacattctta acttgcttcg
     2701 actgcctact gcctgctgcc tactgcctac tgttttatgc ttgaactcga cggccaacaa
     2761 ttttctctcg aacaagtcgg ggcggtggcg cgcggcgcgg agggcgtgcg gctggcggcg
     2821 gcggcgcgcg ggcggatgga ggcttcgcgc cggacggtgg agcgcatcat cgaggagggg
     2881 cgcgtcgtct atggcgtgaa cacgggcttc ggcaagctgt cggacatctc gatcccgccg
     2941 caggagctgc gcgagcttca gatcaacctc gtgcggagcc acgcgggcgg cgtcgggccg
     3001 gcgctctccg aggcggagac gcgcgcgatg atgttgctgc gcgcgaacgt gctcgcgctc
     3061 ggctacagcg gcgcgcgcgc gtcggtcgcc gagacgctgc tcgccatgct cgaacgcggc
     3121 gtgcacccgt tcatccccga gaagggttcg gtcggcgcga gcggcgatct ggcgccgctg
     3181 tcgcacctcg cgctcgtgtg cgtcggcgag ggcgaagcgc gttatcaggg cgagacgctc
     3241 gcgggcggcg aggcgatgcg gcgcgcaggg atagagccgc tgacgctgga agcaaaggaa
     3301 ggactcgcgc tgctcaacgg gacgcaagcg ctgaccgccg tcggggcgct cgcgctcgaa
     3361 cgcgccgaac ggctcgcgcg cgtggcggac gtggcgggcg cgatgacgct cgaagccctg
     3421 aagggcacgc ccgccgcctt cgacgaacgc atccacgccg cgcgcccgca caagggccag
     3481 cgcgagacgg ctgcgcgcct gcgcgagctt ttgagcgaaa gcgaaatccg cgcctcgcac
     3541 ctcaccggcg acccgcgcgt gcaggacgcc tactccctgc gctgcatgcc ccaggtgcac
     3601 ggcgcggtgc gcgacgcgct ggcgcacgcg cgcggcgcga ccgagatcga gaccggcggc
     3661 gccacggaca acccgctcgt cttcgcggag acgggcgatg tcatctccgg cggcaacttc
     3721 cacggcgcgc cgctcgccct cgccttcgac tattcggcca tcgcgctcgt ctcgctcggc
     3781 aacatcagcg agcgccgcat cgaacgcctc gtcaacccgg acatgaacga agggctgccc
     3841 gccttcctca cctcgcacgc gggcgtcgcc tccggcttca tggtggcgca gatcgcggcg
     3901 gtcgcgctcc tcaacgagtg caaggtgctc gcgcacccgg ccagcgcgga caacctgccg
     3961 accgacggcg gcaaagagga tcatgtctcg atggggatga cggcggcgac caagctccgc
     4021 tcgatagtcg agaacgtcga gcacgtcctc gccatcgagc tgatgtgcgc cgccgaaggg
     4081 ctggactacc gcgcgcctct ccgaccgggc cgcggggtgc gccgcgcgca cgaagccgtc
     4141 cgcgcacacg tcccccgcct cacgcgcgac cgcgcgctct cggcggattt ccgcaaactc
     4201 gccgaagcca tccgccgcgg cgacttcgac ctctagcccg cgtcgcgcgg cgggcgcgtc
     4261 acacacgccc gccgcacgtc gccggcggca gcaggggggt tgctgacgcg cctcgggggt
     4321 gcgtggccgg ggcttcggtc gcgaccgttc aaatccaacg acaactgccg tcgaattgcg
     4381 cgccctcctc gatgatcagg gagtgggccg cgagtttccc tttgacgctc gccgtgcgcc
     4441 cgagcacgag ttcctgactg gcgctgatgt cgccctcgac cgtcccgttg atcttcgcca
     4501 cggcgacttc gatcaccgcg tccgtcacct gcgcgccggt gcccacgacg agcgtgccgt
     4561 ccgaggagtt gacgcgcccg gagaagttcc cgtcgatgcg cagcatcgac ttgaagcgca
     4621 cctcgcccgt gaacatcagg acatccccga cgaagccgag ccagttgtcc ttcggcgtgt
     4681 tcgtcgggtg ctcccgttct tctcttctcg ccaccgcttg actactcacc tcgtcgtcct
     4741 cctttgggtg tgtcccgtcc ggcccttccg aaagatcggg tgatacttcg tcgggccggg
     4801 ccgggaatta gccgcagccg ggagaggcca acctgccttc ggcagtccgt aatcaatgag
     4861 agagttcttc gtcgcgttgg ataaagcgga acgaagtccg agaccgatat tcgagaccga
     4921 tagagggcaa gaacttcttc ggcagaagcc gcggtaagtg tccggcggcg gcacctgaaa
     4981 ccttggccgc cgcctcttcc cttcccgccg aggtgaagag aagaatcagc gtataggcgg
     5041 gcactatagc acccgtcgtg ccgccgagta ttacgattgg attgctgttt ggagacagga
     5101 tggagttgcg cgctcactcc ccggcgcggt cgcggcgtcg gcgcgaaacg gcgggcgtga
     5161 gctaaagcga attgcggttg agtttaccgg gtaaactcaa ccgcaattcg ctccaagcct
     5221 ccgggcggga ggtcgctcag tcgttcacca cctgtggtaa gcggggtggc cggggcggac
     5281 ggcgacgaag gtgccgcggc aggtggcgca gagtttgccg cccgcgacca gttcggcctc
     5341 gaccgtggcg cggtcgccgt tcgattcgac gacgcgcgcg gacagcgcga cggggccgtc
     5401 ggtgggggtc gggcgcagga gtttgacggc gtagtcggcg gtgacggtgc agggcgggcg
     5461 cgggagacgg tcgcgcttca tgaagtggta ggcggcggcc cagttcgagt ggcagtcgag
     5521 cagcgcgccg acgatgccgc cgttgagcac gccggggaaa gcctcgtaca cggcggcggg
     5581 cgtccacacg gcgacgatct gctcgccgtc ctcggacggg aagctcctga cgtgcagccc
     5641 cttctcgttc gccgggccgc agccgaagca ggcgccttgc ggcgaatact tctcttgtaa
     5701 gctcaggtcg gacatttcgg tcactcgtca gcggtcgatg ggcgggtcgg cggcggtggc
     5761 cggttcgccc ttgtggttgg cggcgcggac gatgccggtc tcgtccgtgt agaaggagcg
     5821 gcggcccgtc ttgccgtagt tgcggggggt ggcggtggcg gtgaacttgt cgcccgaggc
     5881 gtgcagctcg taggcgtagt ctccgtcgct cccttcactg gtctgtagga gcttggcctc
     5941 gtacagctct tcgaggctgc cgtagcggcc cttgcctttg ccgtccttga actgctgctg
     6001 cgccgagacg atgcgctgga ggaagtaggt ggcgaaggtc tcgttgccga ggacgggcgc
     6061 ctccttgatg ccgatgacgg tggaggcggc gaagactttg acgaggccga cgggcaggcg
     6121 cagctcgtgc agcagggcgt cgccctcgtt cgtcgcggcg taggaggcgg cgctgagggg
     6181 cacgtcgagc tgcgcgagcg cggcgtggac gaccgggtct tcgctgccgt cgcccatctt
     6241 cttcgcgccg tcgatccagt cgcgcatcag gccgtcggag aggtaggcga gcgcgaggcg
     6301 ctgcggctcc tgccacgcga cggcgtcgcg gaagcggttg ctggcggcga gcgtctggtt
     6361 cgacgagtaa gcgtcgatga cgtggcgcac tgcgggcgcg ctctcgccga cgacgaggaa
     6421 gttgttgacg aaggcgtagg cgagggcgcc gaggtcgtga acctcgaagc ccttgcgccg
     6481 ctcggtcgcc gccgacgcct gcacgggaag gcccatcccc gtgagcagga gcggcactac
     6541 cttgcgcgcg gcttccgggt tgttcagcga gacgagcgcg accggcccgg ccttcgcagg
     6601 cttctcgccc gagctttcgg cctcgcgcgc ggcgtcggta atgaagccgc gccgcccctc
     6661 gaaccaatcg accggcgtgc tcacggcgat ctcgttgccg a
//
LOCUS       DBNGOH010000525         2875 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_260576, whole genome shotgun sequence.
ACCESSION   DBNGOH010000525 DBNGOH010000000
VERSION     DBNGOH010000525.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2875)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2875)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2875
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_260576"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            232..813
                     /locus_tag="AC159P_10250"
     CDS             232..813
                     /locus_tag="AC159P_10250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695872.1"
                     /translation="MFLDPEHPRHQGRRARFPTALALLLTLCALAAACGDQTEQANKL
                     VAEMNNLVVRGEGMARQGTAKQEELEAKDIEKERDAVRAGAREQATLFKQAADSFREA
                     AAKADEAAKLKLADWYKNYLSLKAQQHRKTAELLDAALERAELLAGDQPLEELDAKLS
                     QSAERVARLSKEEEELVKQVKRVEEEHKADFTP"
     gene            904..1230
                     /locus_tag="AC159P_10255"
     CDS             904..1230
                     /locus_tag="AC159P_10255"
                     /inference="COORDINATES: protein motif:HMM:NF018894.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HIR3695873.1"
                     /translation="MGAERRKAKRFRVNLPARWEGLRARDQGTISDLSLTGCFVLTAG
                     RTERGEAIRLAMQLPGGVRISLGAEVVYYTEEIGFAVQFTEGEAADKRQLAAFLKRKQ
                     AEEGDA"
     gene            1276..2826
                     /locus_tag="AC159P_10260"
     CDS             1276..2826
                     /locus_tag="AC159P_10260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate-CoA ligase family protein"
                     /protein_id="HIR3695874.1"
                     /translation="MTINIEFPERFNMADYFLYHNVEEGREGKTCLYYGDDQYTYADA
                     VRLSNRAGNALRESGLEVEDRVLLVLPDCPEFAWAWFGASKVGGVITMVNPLLPAEDY
                     RYYLEYTRARVAVVHESLLDNFAQAARGARYLKNVLVVGEERGTFHSFAEAINAQPDE
                     LVPADTSRDDIAIWLFTSGSTGHPKGAVHLHHDLPYNTECYAKRVLGVNENDLTVSVP
                     KLFFGYATGTNLLFPFAVGGATALFSERSTPEKLFEVVERYRPTILTSVPTMINGMLN
                     VEGAAGHDLSSLRMCLSAGEALPVELYRRWVETFGVEILDGIGSAEMFHIYITNRLGD
                     VVPGSLGRVVEGYEAEVVDATGQPVAAGEMGTLRVKGDSAALCYWQAHEKSKATFAGD
                     WCTTGDQFHADEAGYYWYHGRTDDMLKVSGIYVAPAEIENCLLGHESVLEAAVVGHDA
                     GDGLTKPKAFVVLREGHAPSDPLAQALKDFVKTHLAPYKYPRWIEFAPSLPKNDRGKI
                     DRKALKAQ"
BASE COUNT          525 a         1016 c          945 g          389 t
ORIGIN      
        1 ccggcggcag cgagggcgtc ggctacctgc gcaccacgct cgacaagaag ttcttccccg
       61 agctgtggga ggcgcgcacc tacctcgaca cgcgccacgg cgccgccggg tgccccttcg
      121 ccggccacgc cgagtgagcg cgccgcgtcc ccgaaaaatc cggcgcccgc cgcctcgcct
      181 ttgtggtaag ttcttgccgg cccgaacgtc cactcgcagg agaccgcgtc gatgtttctc
      241 gaccccgaac acccccgaca tcagggacgc cgcgcgcgct tccccacggc cctcgcgctg
      301 ctgctcacgc tgtgcgccct cgccgcggct tgcggcgacc agaccgagca ggccaacaag
      361 ctggtcgcag agatgaacaa cctcgtcgtc aggggcgagg ggatggcccg gcagggcacg
      421 gccaaacagg aggagctgga ggcgaaggac atcgagaagg agcgcgacgc cgttcgggcc
      481 ggggcgcgcg agcaggcgac gctcttcaag caggccgccg actcgttccg cgaggccgcc
      541 gccaaggccg acgaggcggc caagctcaaa ctcgcggact ggtacaagaa ctacctctcg
      601 ctgaaggcgc agcagcaccg caagaccgcc gagcttctcg acgccgcgct cgaacgcgcc
      661 gagctgctgg cgggcgacca gccgctcgaa gaactcgacg cgaagttgtc gcagtccgcc
      721 gagcgcgtcg cccgcctctc caaagaggag gaggagctgg tcaagcaggt gaagcgggtc
      781 gaggaggagc acaaggccga cttcacgccg tagcctccgc gaagggggcg cggcgagtcc
      841 gcctcacgcg cgcgaaccgc gggccgcacg cacacacgcc gcggggacgc caccgacttg
      901 tacatgggcg cagaacgtcg caaggccaaa cgcttcaggg tgaacctgcc ggcgcgctgg
      961 gagggcctgc gcgcccgcga ccaggggacg atcagcgacc tcagcctgac cggctgcttc
     1021 gtgctcacgg cgggccgcac cgagcgcggc gaagcgatcc gcctcgccat gcaactgccc
     1081 ggcggcgtcc gaatcagcct cggcgccgag gtggtctact acaccgaaga gatcggcttc
     1141 gccgtccagt tcaccgaagg cgaggccgcc gacaaacgcc agctcgccgc cttcctcaag
     1201 cggaagcagg ccgaggaagg cgacgcctga ccgaggcgct tgacgaaacc aagcggcggc
     1261 cctgcccgat tagccatgac catcaacatc gagttccccg aacgcttcaa catggccgac
     1321 tacttcctct accacaacgt ggaggagggg cgcgaaggca agacgtgcct gtactacggc
     1381 gatgaccagt acacctacgc cgacgcggtg cggctctcga accgcgccgg caacgctctg
     1441 cgcgagtcag gtctggaggt ggaagaccgc gtgctgctcg ttctgccgga ctgcccggag
     1501 ttcgcgtggg cctggttcgg cgcgagcaag gtcggcggcg tcatcacgat ggtcaacccg
     1561 ctgctgcccg ccgaggacta tcgctactac ctcgaataca cgcgcgcgcg cgtggcggtc
     1621 gtccacgagt cgctgctcga caacttcgcg caggccgcgc ggggcgcgcg ttacttgaag
     1681 aacgtgctgg tcgtagggga agagcgcggc acgtttcact cgttcgcgga agctatcaac
     1741 gcacagccgg acgaactcgt gcccgccgac acttcgcgcg acgacatcgc catctggctc
     1801 ttcacgtccg gctcgaccgg ccacccgaag ggcgccgtcc acctccacca cgacctgccg
     1861 tacaacaccg agtgctacgc gaagcgcgtg ctcggcgtga atgaaaatga tttaacggtc
     1921 tcggtgccca agctcttctt cggctacgcg acggggacga acctgctgtt ccccttcgcc
     1981 gtcggcggcg cgaccgcgct cttctccgag cgctcgacgc cggagaagct gttcgaggtc
     2041 gtcgagcgct accgcccgac gatcctgacc agcgtgccga cgatgatcaa cggcatgctc
     2101 aacgtcgagg gggccgcggg gcacgacctc tcatctctgc gcatgtgcct gtcggcgggc
     2161 gaggcgctcc ccgtcgaact ctaccgccgc tgggtcgaga cgttcggcgt cgaaatcctc
     2221 gacggcatcg gctcggcgga gatgttccac atctacatca cgaaccggct cggcgacgtc
     2281 gtgccgggca gcctcgggcg cgtcgtcgag ggctacgagg ccgaggtcgt ggacgcgacc
     2341 ggccagccgg tcgccgcggg cgagatgggg accctgcgcg tcaagggcga ctcggcggcg
     2401 ctctgctact ggcaggcgca cgagaagtcc aaggcgacct tcgcgggcga ctggtgcacg
     2461 accggcgacc agttccacgc ggacgaggcg ggctactact ggtatcacgg gcgcaccgac
     2521 gacatgctca aggtctcggg catctacgtc gcgcccgccg agatcgagaa ctgcctgctc
     2581 gggcacgagt cggtcctcga agcggcggtc gtcggccacg acgcgggcga cggcctcacc
     2641 aagcccaaag ccttcgtcgt gctgcgcgaa ggccacgccc cgtccgaccc gctcgcgcaa
     2701 gccctcaagg acttcgtcaa gacgcacctc gcgccctaca agtacccccg ctggatcgag
     2761 ttcgccccct cgctccccaa gaacgaccgc ggcaagatcg accgcaaggc gctcaaagcg
     2821 cagtaggcag caggcagtag gcagtcaaag caaaaagcaa gaggcttgag atgta
//
LOCUS       DBNGOH010000526         2675 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_445264, whole genome shotgun sequence.
ACCESSION   DBNGOH010000526 DBNGOH010000000
VERSION     DBNGOH010000526.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2675)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2675)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2675
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_445264"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..628)
                     /locus_tag="AC159P_10265"
     CDS             complement(<1..628)
                     /locus_tag="AC159P_10265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695875.1"
                     /translation="MYSVFRRYSIVARAALLLACAALPPAAAAQTPTPTPTTRPTTTQ
                     EAPKKSEPAKPEKAPKRNEQAAPTAEQVAETVILVHGVRERLAQVRRSGVERGRISRT
                     TDDGRTEEITYERSFKRGENSEKDKIRLDQKRPNIEYALVYNEGRVFGVLRGTAFTPR
                     QDDVSVFLADTRHGVDTLLRYKENGATLAYVGKEKQKNIDMWLLDLTDK"
     gene            977..2239
                     /locus_tag="AC159P_10270"
     CDS             977..2239
                     /locus_tag="AC159P_10270"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HIR3695876.1"
                     /translation="MKKVGILVGREVTFPVSLIDSVNARGGGEVVAEFVKVGGVRHDG
                     GRDYDLIIDRISHQVPFYRAYLKRAALEGTVIINNPFWWSADDKFFNYSLAEKLGVAV
                     PRTVLLPQKDYMEDITGDSLRNLQFPLDWQGIVDYVGFPAFIKPFDGGGWKNVSKINS
                     LEELWEVYDTTGQLCMTLQQGIEFDQFVRCYCIGQQDVMIMPYDPRKAYLSGEQYLHD
                     PNYLTAELAERVGNDVRTLCRALGYDINTVEFAIKDGTPYAIDFMNPAPDAEFASVGE
                     FYHNWLTETVTDLVFRRLAEGQPAPRYRWDAMLNPPAAGSAASALATAASSAASNAAS
                     TVETYIPDSVREVSSDLAAAASELVGTAREAVAEIISTVTAAAATEPTTASKTAKPRR
                     SAAGAAKPKSTTTRKSASPKSKKPSSEA"
     gene            complement(2361..2558)
                     /locus_tag="AC159P_10275"
     CDS             complement(2361..2558)
                     /locus_tag="AC159P_10275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695877.1"
                     /translation="MSDDQVLANQQTILANQEEIKANQQQIRSNQDKLDRILANQEQI
                     KKNQEQILANQERILSHVKGA"
BASE COUNT          443 a          919 c          820 g          493 t
ORIGIN      
        1 ccttgtccgt gaggtcgagg agccacatgt cgatgttctt ctgcttctcc ttgccgacgt
       61 aggcgagggt cgcgccgttc tccttgtagc ggagcagcgt atcgacgccg tgccgcgtgt
      121 cggcgaggaa gaccgacacg tcgtcctgcc gcggcgtgaa ggccgtgccg cgcaagacgc
      181 cgaagacgcg cccctcgttg tagacgagcg cgtactcgat gttgggccgc ttctggtcga
      241 ggcgaatctt gtccttctcg gagttctcgc cgcgcttgaa gctgcgctcg taggtgatct
      301 cctcggtgcg accgtcgtcc gtggtgcgcg agatgcggcc ccgctcgacg ccgctgcgcc
      361 ggacttgcgc gaggcgctcg cgtacgccgt ggacgaggat gacggtctcg gccacctgct
      421 cggcggtcgg ggcggcctgc tcgtttctct tcggcgcctt ctcgggcttc gccggctcgc
      481 tcttcttggg cgcctcctgc gtggtcgtcg gcctagtcgt cggcgtgggc gtgggcgtct
      541 gcgcggcggc ggcggggggc agcgcggcgc aggcgagcag gagggccgcg cgcgcgacta
      601 tcgagtagcg tcggaacact gagtacatct ccttagcttt cttctggttt gtcctggcga
      661 ccggcgccgt tgctcctcgg cgctcgacag gagaggtcgg ccaaatcttc gcgggattgt
      721 aacacacggg cggcctgaat ccggcccccg gccaactgcc cgcgccgccg ttcataacta
      781 ttgatgcccg ccggtttggc gcggatgcct taccccgctg gtttgacaag gttccgccgc
      841 aaaagctaga gttcagcgtt ttcatagaca ccagttccgt gcggcgggcg gccccgatcc
      901 atgatcgaca taattccctt gccgccccac ggcacgcgag cctgccatca tcatccctga
      961 aaggaaatcg aagaccgtga aaaaagtagg cattctcgtc gggcgcgagg tgacattccc
     1021 cgtctcgctc atcgacagcg tcaacgcgcg cgggggcggc gaggtggtcg ccgagttcgt
     1081 caaggtcggc ggcgtgcgcc acgacggagg ccgcgactac gacctcatca tcgaccgcat
     1141 ctcgcaccag gtgcccttct accgcgccta cctcaagcgt gccgcgctcg aaggcaccgt
     1201 catcatcaac aaccccttct ggtggtctgc ggacgacaag ttcttcaact actcgctggc
     1261 cgaaaagttg ggcgtcgcgg tgccgcgcac ggtgctcctg ccgcagaagg actacatgga
     1321 ggacatcacc ggcgactctc tgcgcaacct ccaattcccg ctcgattggc agggcatcgt
     1381 ggactacgtc ggcttccccg ccttcatcaa gcccttcgac ggcggcggct ggaagaacgt
     1441 ctcgaagatc aacagcctcg aagagctgtg ggaggtctac gacaccacgg gccagctctg
     1501 catgaccttg cagcagggca tcgagttcga ccagttcgtc cgctgctact gcatcggcca
     1561 gcaggacgtg atgatcatgc cctacgaccc gcgcaaggcg tacctctcgg gcgagcagta
     1621 cctgcacgat cccaactacc tcacggcgga gctggccgag cgcgtcggca acgacgtgcg
     1681 cacgctctgc cgcgccctcg ggtacgacat caacaccgtc gagttcgcca tcaaggacgg
     1741 cacgccttac gccatcgact tcatgaaccc cgcgcccgac gccgagttcg cctcggtcgg
     1801 cgagttctac cacaactggc tcaccgaaac ggtcaccgac ctcgtcttcc gccgcctcgc
     1861 ggaaggtcag cccgcgccgc gctaccgctg ggacgcgatg ctcaacccgc ccgccgccgg
     1921 ctccgccgcg agcgcgctgg ccaccgccgc ttcgtccgcc gcgtcgaacg ccgcctcgac
     1981 ggtcgagacc tacatccccg actcggtgcg cgaagtctcc tccgacctcg ccgccgccgc
     2041 cagcgaactc gtcggcaccg cccgcgaggc cgtcgccgaa atcatcagca ccgtcaccgc
     2101 cgccgccgcg accgagccga ccacggcctc caagaccgcc aagcccaggc gctccgccgc
     2161 cggcgccgcc aaacccaaga gcacgacgac caggaagagc gcttcgccca agtcgaagaa
     2221 accttcttcc gaagcgtagc cacgcgctcc gcgtcgtaca cgaacgaaaa gggcgaccca
     2281 cggcgggtcg cccttttcgt taacggtggc cgggggcagc agcgaatcgc cgcgagcgcg
     2341 actttcaggc tcaagtctcc ttacgcgcct ttcacgtgcg agagtatccg ttcctgattg
     2401 gcaaggattt gctcctgatt tttctttatc tgctcctggt tcgcaagaat ccgatccagc
     2461 ttatcctggt tggagcggat ctgctgttgg ttggccttga tctcttcctg attggcaagg
     2521 atggtttgct ggttcgcgag cacctgatcg tcggacatga agggaccgcc tcctgcgttg
     2581 gatgtgtcga acgctttatt aatacccgaa gccgcgctat gtgaacaggg cggactatcg
     2641 aagtcgggtt acggacgagg aaagccgccg gagct
//
LOCUS       DBNGOH010000527         3112 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4659589, whole genome shotgun sequence.
ACCESSION   DBNGOH010000527 DBNGOH010000000
VERSION     DBNGOH010000527.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3112)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3112)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3112
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4659589"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..435
                     /locus_tag="AC159P_10280"
     CDS             <1..435
                     /locus_tag="AC159P_10280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695878.1"
                     /translation="GRRRAPAAQPTPTPPDSAGEEAAARPSEETPRPEEGTPESTARP
                     PAERPPASTARRRGSRATNRRRAPDPPPAEAAGGAAPAEPPPVVTITRLVILTKDGQT
                     LERDMDTVRRVTIENNQIVVVLKDGKVVRTPLAIVSKMSFEP"
     gene            603..1343
                     /locus_tag="AC159P_10285"
     CDS             603..1343
                     /locus_tag="AC159P_10285"
                     /inference="COORDINATES: protein motif:HMM:NF020893.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HIR3695879.1"
                     /translation="MPLAFSKSGGRWPRILLTVAVVCVIFAVNRWSGLWELPERLREL
                     GVGGRTAALIILVMAFMGAFALPASAFLFITPLLFPPRVSALITTAGFAVSTGVGYAV
                     ARFVGGPWVERSRDGRLRRFLARHSSFLVLFGIRLTPSSPHGFISYAAGLAAIPFVRF
                     LVATTAAMAIKSYVYAEAVHGTVGAKSLTDVVSAKTLLAMSSVALLAFVGHALGRRFA
                     RATAETGRRGDEEKGRRGEGVRSLLPGD"
     gene            1511..2596
                     /gene="bchI"
                     /locus_tag="AC159P_10290"
                     /pseudo
     CDS             1511..2596
                     /gene="bchI"
                     /locus_tag="AC159P_10290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012282386.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0010007 - magnesium chelatase complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016851 - magnesium chelatase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0015995 - chlorophyll biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase ATPase subunit I"
BASE COUNT          521 a         1139 c         1006 g          446 t
ORIGIN      
        1 ggcaggaggc gcgcgccagc cgcgcagccc acgccgacgc cgccggactc tgccggcgaa
       61 gaggccgccg cgaggccgtc cgaagaaacg ccgaggccgg aagaagggac gcccgagagc
      121 acggcgaggc cgcccgccga gaggccgccc gcgagcaccg cgcggcgccg cggctcgcgc
      181 gcgacgaaca ggaggcgggc gcccgacccg ccgcccgccg aagccgcggg cggagcggcg
      241 cccgcggagc cgccgcccgt cgtcaccatc acgcgcctcg tcatcctgac gaaggacggc
      301 cagacgcttg agcgcgacat ggacaccgtg cggcgcgtga ccatcgagaa caaccagatc
      361 gtcgtcgtct tgaaggacgg caaggtcgtc cgcacgccgc tggcgatagt ctcgaagatg
      421 tccttcgagc catgaagtaa tcgagaaggg gagacgggga gaaaaggaga aggagagaaa
      481 gggagtgagg gagaagggga gaaagagata aagcagacgt tcgacatcac agcttctttt
      541 tctccccact gcctttctcc gccactccgc cactcccttt ctccctttct gcttgataga
      601 ggatgcctct ggccttttca aagagcggcg ggcgctggcc gcgtatcctg ctgaccgtcg
      661 cggtggtgtg cgtcatcttc gcggtcaacc gttggagcgg gctgtgggag ttgccggagc
      721 gtctgcgaga actcggcgtc ggcgggcgca ccgcggcgct catcattctg gtgatggcgt
      781 tcatgggagc gttcgcgctg ccggcctcgg cgttcctctt catcactccg ctgctgttcc
      841 cgccgcgcgt gtcggcgctg attacgaccg ccggtttcgc ggtcagcacg ggggtcggct
      901 acgcggtcgc gcgcttcgtc ggcggcccgt gggtcgaacg ctcccgcgac ggtcggctgc
      961 gccgcttcct cgcgcgtcat tcgagcttcc tcgttctctt cggcatccgc ctgacgccct
     1021 ccagcccgca cggctttatc agctacgccg cagggctagc cgcgataccc ttcgtgcgct
     1081 tcctggtggc gacgaccgcc gcgatggcga tcaagtccta cgtctacgcc gaggccgtcc
     1141 acggcacggt cggcgcgaag tctttgaccg acgtggtctc ggcgaagacc ctgctcgcca
     1201 tgtcctcggt cgcgctgctc gccttcgtgg gccacgcgct cggcagacgc ttcgcgcgcg
     1261 cgacggctga gacggggaga cggggagacg aggagaaggg gagacgagga gaaggggtgc
     1321 gaagcctttt acctggcgac tgaggtcgaa tcacttgcgc gacgctcagt cactacacag
     1381 ttaatcacct cgtctcccca tctccccgtc tccctgtctc cccttctgtt cgatagtggc
     1441 tttcggcatg aagtgattta taatccggcg cgcaccaccc gctcgcgtcc gacacggact
     1501 cgcacacacg atggcagggc gtcccaacca acacccaacg cgcagcaccg gctcacgcgc
     1561 gacgcggccc gtctacccct tcacggcgat agtcgggcag gaggagatga agctcgcgct
     1621 cctcttgaac gtcatctcgc ccgcggtcgg cggcgtcctc gtcatgggcc accgcgggac
     1681 ggggaaatcg accgccgtgc gcgcgctcgc cgaactcctc ccgccgctca cctccgtccg
     1741 cgactgcact tacaactgcg acccgcgcga cgttgacgcc gcgtgcgccg actgccgccg
     1801 ccgcctcgca gcgggcgaga ggttgaagcg tgagcagagg ccggtgcccg tcgtcgagct
     1861 gccgctcggc gcgaccgaag accgcgtgtg cggcaccatc gacatcgagc gcgcgctcaa
     1921 ggaaggcgtc aaggcgttcg agccgggcct gctcgcgcgc gccaaccgcg gcttcctcta
     1981 catcgacgaa gtgaacctgc acgaagatca tcttgtcgat gtgctgctcg acgcggcggc
     2041 gaccggcacg ggccgcgtcg agcgcgaagg cgtctccgca gagcaccccg cgcgcttcgt
     2101 cctcgtcggc tccggcaatc ccgaagaggg cgagctgcga ccgcagttcc tcgaccgctt
     2161 cggcacctcg gtcgaagtcc agaccatcct tgaccccgcc gcgcgcgtct cgatagtcga
     2221 acagcgcgag gcgttcgagc gcgaccccga aggcttcatc gatgcgcgtc aggcggagct
     2281 tgaagacttg cgccgccgcc tcgtgcgcgc cgcgcgagcc gagcggcgcg tcgagacgcc
     2341 gcgcgaaatt ctgttctcga tagccgagct gtgcgtccgc ctcggcgttg acggccaccg
     2401 cggcgaactc accatcacgc gcgccgcacg cgcgctcgcg gccttcgaag gccggaagaa
     2461 aatcaccgcc gcggacgtgc gccgcgtcgc cccgatgtgc ctgcgccacc gtttgcgccg
     2521 cgacccgctc gaaatggtcg agggcggtgc gcgcatcgaa caagccgtcg aagacatcta
     2581 cggcgaagac ccggaaggcc ccgacgaagg ccacgcgcga cgcggcaacg gccctgacta
     2641 tccgccgcca cactccttcg accacacggg cgccgccccg acgcagaaca cgccgctcga
     2701 aaagaatcgc ggacacgagc gaggctttcg acaggccgac acgggttcca accaccacga
     2761 ccctcatcga ccatccgacc gcgaagcact cgacgccgca ccctcgaccg aagagcgcgt
     2821 catcccttcc gcgaccgcgg cgctacctga aagcgcgctc gactcgcatc gagcgaacac
     2881 ggcgcgcggc agcaagggcc gctcccacgc acgccgaagc gcgggaccga aacgcacctc
     2941 gcccgctccg cgcgggcgct acgcgacggc caccgccgat aaaactcacg gcgcccgcgt
     3001 cgccgttgac gcgacactgc gccgcgcctc ggtcgcacag tccgcgcgtc acgcatcagc
     3061 cacacacgcg gcgctgcccg accccgccgc gcctaagcta cgcgtgcgcg cg
//
LOCUS       DBNGOH010000528         2484 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_261177, whole genome shotgun sequence.
ACCESSION   DBNGOH010000528 DBNGOH010000000
VERSION     DBNGOH010000528.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2484)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2484)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2484
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_261177"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            61..1062
                     /locus_tag="AC159P_10295"
     CDS             61..1062
                     /locus_tag="AC159P_10295"
                     /inference="COORDINATES: protein motif:HMM:NF014893.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR3695880.1"
                     /translation="MTKVKWGLLGCGDIARRRVAPALGDLPHCELVAVSRARAELAAE
                     FAAEFGARRWYADWRALLADEEVGAVYVATPVRLHAAQTIAAAEGGKHVLCEKPMALD
                     VAECDRMVAACAANGVRLGVAYYRRFYPVVERIRAIIASGEIGRPSVAQLNAFEWFDP
                     APAHPRRWLLDKGQSGGGPLFDFGCHRVEVLINLFGAARRVGGFAANVHFDREVEDTA
                     SALFDFDGGPHAVLTVTHAAREPQDTLDIFGTEGSVRVPVLNAGQLHVKTRDGERTES
                     HPPHHNLHQPLIDDFARAVLEGRDPAVDGAVGREVSRLIEEIYQGEEGKRGKGEEGG"
     gene            complement(1189..>2484)
                     /locus_tag="AC159P_10300"
     CDS             complement(1189..>2484)
                     /locus_tag="AC159P_10300"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HIR3695881.1"
                     /translation="IRPPRFPARDFGITDHGARGDGRTDNTDAIRKAINAAHAAGGGR
                     VVVPAGTFLTGAIHLKSNVNLHVSEGATLKFSTDPAKYLPLVYTRFEGTECMNYSPLI
                     YAFEQENIAVTGKGTLDGSASDENWWGWARRGPGGQRSRAHEDIRRLREMAERGVPVK
                     ERVFGPGYHLRNSFIQPYRSRNILVEGIKIINSPFWEIHPVLSQNVTVRGVHIDTHGP
                     NNDGCDPESSRDVLIEDCVFDTGDDCIAIKSGRDADGRRVNVPSENIIIRNSTMKDGH
                     GGVVIGSEISGGARNVFVEDCKMDSPNLDRALRFKSNAIRGGVVENVFMRNVEVGRVS
                     EAVLTIDLLYDTGDKGPHKPVVRNVLIENVTSRSSPRVMWIVGFPGVRIDNIRFVNCT
                     FRGVETAEVMNHAGSFSFENVTIEPAKKGRSQNSPQSQE"
BASE COUNT          371 a          829 c          841 g          443 t
ORIGIN      
        1 gtctccccct ttcacctctt ccccttttgc ccttttcccc gtctgtcgaa ggagaccggc
       61 atgacgaaag tgaagtgggg actgctgggc tgcggcgaca tcgcgcgcag gcgcgtcgcg
      121 ccggccctcg gcgacctgcc gcattgcgag ctggtcgccg tcagccgcgc gcgcgcggaa
      181 ctcgccgcgg agttcgccgc ggagttcggc gcgcgccgct ggtacgcgga ctggcgcgcg
      241 ctgctcgcgg acgaagaggt cggcgccgtc tacgtcgcca cgcccgtccg cctgcacgcc
      301 gcacagacaa tcgccgccgc cgaaggcggc aagcacgtcc tctgcgaaaa gccgatggcg
      361 ctcgacgtgg cggagtgcga ccggatggtg gcggcctgcg ccgcgaacgg cgtgcggctc
      421 ggcgtcgcct attaccggcg cttctacccc gtggtcgaac gcatcagggc gatcatcgcg
      481 tccggcgaga tcgggcgccc gtcggtcgcg cagctcaacg ccttcgagtg gttcgacccc
      541 gcgccggcgc acccgcgccg ctggctcctc gacaagggac agtccggcgg cggcccgctg
      601 ttcgacttcg gctgtcaccg cgtcgaagtc ctgatcaacc tcttcggcgc ggcgcgtcgc
      661 gtcgggggct tcgccgccaa cgtccacttc gacagggaag tggaggacac ggcgagcgcg
      721 ctcttcgact tcgacggcgg cccgcacgcc gtcctcaccg tcacacacgc cgcgcgcgag
      781 ccgcaggaca cgctcgacat cttcggcacc gaaggctccg tccgcgtccc cgtcctcaac
      841 gccggtcagc tccacgtcaa aacccgcgac ggcgaacgca ccgaatccca ccccccgcac
      901 cacaacctcc atcaaccgct catcgacgac ttcgcccgcg ccgtgctcga aggacgcgac
      961 cccgcagttg acggcgcggt cgggcgcgag gtgtcgcggc taatcgaaga gatttatcaa
     1021 ggggaagagg ggaaaagggg aaaaggcgaa gagggaggat gagaggcacg ctgcttctcg
     1081 tcggcaaccg aacatgaaaa gcagcgtgcc tctcatcact cgttgatgag aggcacgccg
     1141 ctttttgtct tcccttttgg ccttttcgcc ttttcccctt ttcgctcttc attcttgaga
     1201 ttgcggcgag ttttggctgc ggcctttctt cgcgggttcg atggtgacgt tctcgaagga
     1261 gaaggagcct gcgtggttca tgacctctgc ggtctcgacg ccgcggaagg tgcagttgac
     1321 gaagcggatg ttgtcgatgc ggacgccggg gaagccgacg atccacatca cgcgcgggct
     1381 ggagcggctc gtcacgttct cgatcaggac gttgcggacg accggcttgt gcggcccctt
     1441 gtcgcccgtg tcgtagagca ggtcgatggt gagcacggcc tcggagacac ggcccacctc
     1501 gacgttgcgc atgaagacgt tctcgaccac gccgccgcgg atggcgttcg acttgaagcg
     1561 cagcgcgcgg tcgaggttcg ggctgtccat cttgcagtcc tcgacgaaga cgttgcgcgc
     1621 gccgcccgaa atctcgctgc cgatgacgac gcccccgtgc ccgtccttca tcgtcgagtt
     1681 gcggatgatg atgttctccg aagggacgtt gaccctgcgc ccgtcggcgt cgcggccgga
     1741 cttgatggcg atgcagtcgt cgcccgtgtc gaagacgcag tcctcgatga gcacatcgcg
     1801 cgaggactcg gggtcgcagc cgtcgttgtt cggcccgtgc gtgtcgatgt gcacgccgcg
     1861 caccgtgacg ttctgcgaga gcacggggtg aatctcccag aagggcgagt tgatgatttt
     1921 gatcccctcg acaaggatgt tgcggctgcg gtagggctgg atgaagctgt tgcgcaggtg
     1981 gtagccgggg ccgaagacgc gctccttgac gggcacgccg cgctcggcca tctcgcgcag
     2041 gcggcggatg tcttcgtgcg cgcgcgaacg ctggccgccg gggccgcgcc ttgcccagcc
     2101 ccaccagttc tcgtccgacg ccgagccgtc gagcgtgccc ttgccggtga cggcgatgtt
     2161 ctcctgctcg aaggcgtaga tgagcggcga gtagttcatg cactccgtcc cctcgaagcg
     2221 cgtgtagacg aggggcaggt acttcgccgg gtcggtgctg aacttgagcg tcgcgccttc
     2281 ggagacgtgg aggttgacgt tgctcttcag atgaatcgcg ccggtgagga acgtgcccgc
     2341 gggcacgacc acgcgtccgc cgcccgccgc gtgcgccgcg ttaatcgctt tgcggatggc
     2401 gtcggtgttg tccgtcctgc cgtcgccgcg cgcgccgtgg tcggtgatgc cgaaatcgcg
     2461 cgcggggaaa cggggcggcc tgat
//
LOCUS       DBNGOH010000529         4181 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4659639, whole genome shotgun sequence.
ACCESSION   DBNGOH010000529 DBNGOH010000000
VERSION     DBNGOH010000529.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4181)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4181)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4181
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4659639"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..477
                     /locus_tag="AC159P_10305"
     CDS             <1..477
                     /locus_tag="AC159P_10305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695882.1"
                     /translation="SFLAGLSLVATLNAVFDPLGPAPASPSAAALPARPTSEAAADKD
                     RQHAGAAVREGGASPATSDSDRDTQTAAGQAPAPVPAAAAVIGTRRAEGLPASRREYV
                     VQVLVEVKDGRVTDARVWNPRPGAGAYEAVALKLARERTYPASFNGGERLRISVKP"
     gene            549..2042
                     /locus_tag="AC159P_10310"
     CDS             549..2042
                     /locus_tag="AC159P_10310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695883.1"
                     /translation="MLFIQSNSLALSPTEGERLRLLDALAARGFVPLVHTLRRRMFQS
                     LVASDGDEAFVATAAKELERECAARHESDGEGAYTPGELFCAGDCVLSLLFREEAEED
                     GGVIAGVVYSVDTSEPLMRLERFCRDVREALDEIVDERSQRAGSRRDEERGAEPATAT
                     AAGSTDWQARGARSAHGLARFIATQPVDLVRAAALRGGRGLTRASELMEGREVRLFLR
                     RVEEMRHEGYTPRRLLREADALGGVSVETMLEAGLLEREVRVSCRKSGHALFDLPSPD
                     SLAAITISKAKCSLCAAPVADEVVEETFAPTRLAAALLEDGRWLSNRVHKIVRSLGVP
                     DSEIAIGPASAHGESHLAVEVCGSSFLFVTRDGDLTPALARRVVEVAEETEATHLVVI
                     TTGAVEDEGHLRLYEFAWRRARAGRDLDLTLVKGLGDARALIEQAFERAVCREMSRHL
                     FTLDAALGFGAGNFVLNWFKSVKAAAAPREPEVRAPLLIEFDRQAAS"
     gene            2068..4095
                     /locus_tag="AC159P_10315"
     CDS             2068..4095
                     /locus_tag="AC159P_10315"
                     /inference="COORDINATES: protein motif:HMM:NF015585.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HIR3695884.1"
                     /translation="MRADLKFFLLASSLCLFVLPQWARAEVRVPALIGDNMVLQQGQR
                     VRVWGTGGAGERVTVSVAGETARTNADEDGRWQTFLGPLKAGGPVTLTIAGDRNKLTF
                     RNVLVGEVWVCSGQSNMEWPLAQSTGGHEAAAQGPHTEMRLFTVEKRTSAEPLADVEG
                     RWVVATPEQALRFSAVGYYFGRELHQRLRVPVGLIHTSWGGTPAEAWMSREALAARPE
                     FRPIVERYEKELRNLPQLRREHAAKLAEWERQYVTPDAGNKGEAQGFAEPARDREGWR
                     KMRLPQQWEAAGMNLDGVIWFRRSVEVPTAWAGRDLTLSLGPIDDFDTTYFNGARVGE
                     TGADTPNAWTFPRRYRIPGALVRPGKNVVAVRVYDRMGGGGFGGGGASGAMSLAPVGA
                     GEAGALALEGEWDYKPESVVEAKRLDFSNYPGPRPGPDNQNNPSVLYHAMLAPLTPYA
                     IRGAVWYQGESNAGRAYQYRTLFPALIRDWRARWGQGDFPFYFVQLANWKARKQDSID
                     SEWAELREAQLLTLSSVLRTGMAVAIDIGEAEDIHPRNKLDVGLRLARWALADTYRQT
                     VVPSGPLYVSHSVEGDRIRVRFEHARGLKTSAGSAPAGFAVAGADRKFVPAEARVEGD
                     AVVVWSKDVPRPVAVRYAWADNPAANLYNADGLPASPFRTDDWPGLTAVSY"
BASE COUNT          613 a         1465 c         1528 g          575 t
ORIGIN      
        1 agcttcctcg ccggtctcag tctggtggcg acgctgaacg ccgtcttcga cccgctcggc
       61 cccgcccccg cgtcgccttc ggcggcggcg ctccccgcgc ggccaacgtc cgaggccgcc
      121 gccgacaaag accggcagca cgcgggcgcc gccgtgcgcg agggcggggc gtctccggcg
      181 acttccgact cggatcggga tacgcagacc gcggcggggc aggcgccggc gcccgtgccg
      241 gcggcggcgg cggtgatcgg cacgcggagg gccgagggac tcccggcctc gcggcgcgag
      301 tacgtcgtgc aggttctcgt ggaggtgaag gacgggcgcg tgaccgacgc gcgcgtgtgg
      361 aacccgcggc ccggcgcggg cgcttacgag gcggtcgcgc tgaagttggc gagggagcgc
      421 acctacccgg cgagcttcaa cgggggcgag cggctgagga tcagcgtgaa accgtaacgc
      481 gcgactcccc ttgcgcgcaa aggtcgcgca cggaaggggc ttgcgcgacg cgcgggggcg
      541 gcaaagagat gctattcatc cagagcaaca gtctggcgct gtcgccgacc gagggcgaac
      601 gcctccggct gctcgacgcg ctggcggcgc gcggcttcgt cccgctcgtc cacacgctgc
      661 gtcgccggat gtttcaatcg ctcgtggcaa gcgacggcga cgaagcgttc gtggcgaccg
      721 ccgcgaagga gcttgagcgc gagtgcgcgg cgcggcacga gtccgacggc gagggcgcct
      781 acacgccggg cgaactgttc tgcgcgggcg actgcgtgct ctcgctcctc ttccgcgaag
      841 aggcggagga ggacggcggc gtcatcgcgg gcgtcgtcta cagcgtggac acgtcggagc
      901 cgctgatgag gctcgaacgc ttctgccgcg acgtgcgcga ggcgctcgac gagatcgtgg
      961 acgagcgaag tcagcgggcc ggaagcagac gggacgaaga gagaggcgcg gagccggcga
     1021 cggccacggc ggcgggttcg accgactggc aggcgcgcgg cgcgaggtcg gcgcacgggc
     1081 tggcgcggtt catcgccacg cagcccgtcg atctcgtccg cgccgcggcg ctccgcgggg
     1141 gccgcggtct gacgcgcgcc tccgagctga tggaggggcg cgaggtgcgc ctcttcctgc
     1201 ggcgcgtcga ggagatgcgg cacgagggct acacgccgcg gcggctgctg agagaggcgg
     1261 acgcgctcgg cggcgtctcg gtcgagacga tgttggaggc ggggctgctt gagcgcgagg
     1321 tgcgcgtgag ctgccgcaag tcggggcacg cgctgttcga cctgccttcg cccgactcgc
     1381 tcgcggccat caccatcagc aaggcgaagt gcagcctgtg cgccgcgccc gtggccgacg
     1441 aggtggtgga agagaccttc gccccgacgc ggctcgccgc ggccctgctc gaagacggca
     1501 ggtggctgag caaccgcgtg cacaaaatcg tccgctcgct cggcgtcccc gacagcgaga
     1561 tcgccatcgg ccccgcctcg gcgcacggcg agtcgcacct cgccgtcgaa gtctgcggca
     1621 gctctttcct cttcgtcacg cgcgacggcg acctgacgcc cgcgctcgcg cgccgcgtcg
     1681 tcgaggtcgc cgaggagacg gaggcgaccc acctcgtcgt catcacgacg ggcgccgtcg
     1741 aggacgaggg gcacctgcgg ctctatgagt tcgcttggcg gcgcgcgcgc gccgggcgcg
     1801 acctcgacct gacgctcgtc aaagggctgg gcgacgcgcg cgcgctcatc gaacaggctt
     1861 tcgagagggc cgtctgccgc gagatgtccc gccacctctt caccctcgac gccgcgctcg
     1921 gcttcggcgc cggcaacttc gtgctcaact ggttcaagtc cgtgaaggcc gccgctgcgc
     1981 cacgggagcc ggaggtgcgc gcgcctctgc tcatcgagtt cgaccgtcag gccgcgtcct
     2041 gatccggagt tgtgcgcgtg aggtgtgatg agagcagacc ttaaattctt ccttctcgct
     2101 tcgagcctct gcctcttcgt cctgccgcag tgggcgcgtg cggaggtgcg cgtgcccgcg
     2161 ctcatcggcg acaacatggt cttgcagcag gggcagaggg ttcgcgtctg gggcacgggc
     2221 ggcgcgggcg agcgcgtgac ggtgagcgtc gccggcgaga ccgcgcgcac gaacgcggac
     2281 gaagatggac gctggcaaac tttcctcggc ccgctcaagg cgggcggccc cgtcacgctg
     2341 accatcgcgg gcgacaggaa caagctcact ttcaggaacg tgctcgtcgg cgaggtctgg
     2401 gtctgctcgg gccagtcgaa catggagtgg ccgctcgcgc aatccaccgg cgggcacgag
     2461 gccgcggcgc aaggcccgca cacggagatg cgcctcttca ccgtcgagaa gaggacttcc
     2521 gccgagccgc tcgcggacgt agagggccgt tgggtcgtcg ccacgccgga gcaggcgctc
     2581 aggttctcgg cggtcggcta ctacttcggg cgcgagcttc atcagcgcct ccgcgtcccg
     2641 gtcggcctga ttcacacctc ctggggcggc acgcccgcag aggcctggat gagccgcgag
     2701 gcgctcgccg cgcgccccga gttcaggccc atcgtcgagc gttacgagaa ggagctgcgg
     2761 aaccttccgc aactgcggcg cgagcacgcc gcgaagctgg ccgagtggga gcggcaatat
     2821 gtcacgccgg acgcgggcaa caagggcgag gcgcagggct tcgcggagcc tgcgcgcgac
     2881 cgagagggct ggcggaagat gaggctcccg cagcagtggg aggcggcggg gatgaacctc
     2941 gacggcgtca tctggttccg ccgaagcgta gaggtgccga ccgcgtgggc gggccgcgac
     3001 ctgacgctct cgctcggccc catcgacgac ttcgacacga cctacttcaa cggcgcgcgc
     3061 gtcggcgaga ccggcgccga cacgcccaac gcgtggacgt tcccgcgccg ctaccgaatc
     3121 cccggcgcgc tcgtccgtcc cggcaagaac gtcgtcgcgg tgagggtcta cgaccgcatg
     3181 ggcggcggcg gcttcggcgg cgggggcgcg tccggggcga tgtcgctcgc gcccgtcggc
     3241 gcgggcgagg cgggcgcgct cgcgctcgaa ggcgagtggg attacaagcc ggaatcggtc
     3301 gtcgaggcga agcggctcga cttctcgaac taccccggcc cgcggcccgg ccccgacaac
     3361 cagaacaacc cctccgtcct ctaccacgcg atgctcgcac cgctcacgcc ctacgccatc
     3421 cgcggcgccg tctggtatca gggcgagtcg aacgcgggcc gcgcctacca gtaccgcacc
     3481 ctcttccctg cgctgatccg cgactggcgc gcgcgctggg ggcagggcga cttccccttc
     3541 tacttcgtcc agctcgccaa ctggaaggcg cgcaagcagg actcgataga cagcgagtgg
     3601 gcggagctgc gcgaggcgca gttgttgacg ttgagcagcg tgctgcgcac ggggatggcc
     3661 gtcgccatcg acatcggcga ggccgaagac atccacccgc gcaataagct cgacgtgggc
     3721 ctgcgcctcg cacgctgggc gctcgcagac acctaccggc aaacggtcgt gccctccggg
     3781 ccgctctacg tttcgcactc ggtcgagggc gaccgcatcc gcgtgcgctt cgaacacgcc
     3841 cgcggcctca agacctccgc ggggagcgcg cccgcgggct tcgccgtcgc cggggccgac
     3901 cgcaagttcg tccccgccga ggcgcgcgtc gaaggcgacg ccgtcgtcgt ctggagcaag
     3961 gacgtgccgc gccccgtcgc cgtccgctac gcgtgggccg acaaccccgc ggccaacctc
     4021 tacaacgccg acggcctccc cgcctcgccc ttccgcacgg acgactggcc gggcctgacc
     4081 gctgttagct attagccgga agcaaaaagc gaaggcaggt ggtcgccacg atcggacggc
     4141 ctcctgcctt cttcttgcta acagctaacg gctaacagct a
//
LOCUS       DBNGOH010000530         3917 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2094083, whole genome shotgun sequence.
ACCESSION   DBNGOH010000530 DBNGOH010000000
VERSION     DBNGOH010000530.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3917)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3917)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3917
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2094083"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="AC159P_10320"
     CDS             <1..200
                     /locus_tag="AC159P_10320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695885.1"
                     /translation="VGVTDSPERLGELEQREMLYEKGGVARDQARGDDAAPQLGRHLG
                     GVRPPDEPRLVRWDRRMFVHT"
     gene            complement(595..1422)
                     /locus_tag="AC159P_10325"
     CDS             complement(595..1422)
                     /locus_tag="AC159P_10325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695886.1"
                     /translation="MQKIVALALASLFLFHLAPVALASAPRAPQDGALHQEIPLTNKE
                     VYAMVKAKIAPEVIVAKIESSRCHFETAPNVLAEMRYNGVPDSVLMAMVKAPYGRPAP
                     APATAPKTRPAPQTADEPETFEGPTALLAEGTELTVTPTEEINSKTATEGQVVTFRVV
                     NDVVIDGHTVIPEGAIARGTVVNVEGRGMLGKAGKLAVRVDSVKAVDGQKVRLRAQQA
                     KQGGSNTGKVIALSLLVTPLFLLMRGNTARIKPGQHILVYTDEGKRVGVRDRAVEAK"
     gene            1988..2485
                     /locus_tag="AC159P_10330"
     CDS             1988..2485
                     /locus_tag="AC159P_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HIR3695887.1"
                     /translation="MGLFDRMFGRKATEVEKQPDAQQRFDQLKQKYQSVLNTADQQQI
                     QFHNLHVEGDKLYIKGVAPSDDAKNKFWDQIKLVNPNADDISADISVSAQSAQGAAVG
                     GGQGAGGQSYTVKSGDTLSKISKQFYGDSNEYMRIFYANRGQLSDPDKIQVGQELTIP
                     PDDNA"
     gene            complement(2776..>3917)
                     /locus_tag="AC159P_10335"
     CDS             complement(2776..>3917)
                     /locus_tag="AC159P_10335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695888.1"
                     /translation="TPAFANKSFSADGTVVAATLTYDPNATFTGTSTLIADPSRPGYD
                     TFIIKLTLHEMGHTMGLDHPVVPNGDICAQPDGATVMNYICNINDGANNMPLKLTECD
                     RNAINSETKYPPFTQVPNPIDGSDFFVRQHYLDFLNRGADAGGLAFWVGGIESCGADL
                     QCRAVKRVDTSAAFFLSIEFQETGYFVYRVYQAAFGDLAGKPVPVTIQEFLPEAQTVG
                     RGVVVGVGDWQAQLEANKNAFVGDLSARTRFASRYPATLSPAEFVDALNANAGGALSQ
                     AERDQIVNSLATGALTRAQVLRRVAEDADLARNELNPAFVLMQYFGYLRRNPDDPDFQ
                     GTPDPNFEGFNFWLTKLNDFNGNYVEAELVRAFIESIEYRRRFGQ"
BASE COUNT          703 a         1170 c         1372 g          672 t
ORIGIN      
        1 tggtgggcgt caccgattcc cccgaacgcc tcggcgagct ggagcagcgt gagatgctct
       61 acgagaaggg cggcgtcgcc cgtgatcagg cacggggcga cgatgctgcc ccgcaactcg
      121 ggcggcacct cggcggcgtc cgcccgccgg acgagccacg cctcgtccgc tgggatagaa
      181 gaatgttcgt acacacgtaa ttcgagaaag ctcattcgcg tctcctcgcg gaggccggat
      241 cgttagaacg taagagaggg gcaagagagc ggcggcggcg cgggtggcgt gcgccgtgcg
      301 gtggatatcg ccagccgcgt cctgaggcta atcaactgcc gtgccgtgcg gcggctacgc
      361 ggcgacagcg cggaatgacc gggggccatg aaccgagggc ggcggagagt tcattctgca
      421 cgcgtcagat tgatgccggt ggtcagtttg cgagagtgcc gcgggcatga acgcatgagg
      481 ggttcaaagc ggagagggga gagggaacgc gagggcggcg gcaaaagctg ccggaatctt
      541 cttcatgcgg gcctccagcg gagtgagatg agggcgagcg cggcggcgca ctccttactt
      601 cgcttcgacc gccctatcac gcaccccgac ccgcttgccc tcgtccgtgt agacaaggat
      661 atgctgcccc ggcttgatgc gcgcggtgtt gccgcgcatc aggagaaaca gcggcgtgac
      721 gagcagcgag agggcgatca ccttgcccgt gttgctgccg ccctgcttgg cttgctgcgc
      781 tcgcaggcgc accttctggc cgtccacggc cttcacgctg tccacgcgca cggcgagctt
      841 cccggccttg ccgagcatcc cccgcccttc gacattaacg accgtgccgc gcgcaatggc
      901 gccttccggg atgaccgtgt gcccgtcaat caccacgtcg ttgacgacgc ggaacgtgac
      961 gacctgcccc tcggtggccg ttttgctgtt gatctcctcg gtcggcgtca cggtcaattc
     1021 cgtcccctcg gcgagcaggg cggtcggccc ctcgaacgtt tccggttcgt ccgcggtctg
     1081 cggcgcgggc ctcgttttgg gcgcggtcgc gggggctggc gccggcctac catacggggc
     1141 cttgaccatc gccatcaaca ctgagtcggg cacgccgttg tagcgcatct cggcgaggac
     1201 gttcggcgcc gtctcgaagt ggcagcgcga ggactcgatc ttggcgacga tcacctcggg
     1261 cgcgatcttg gccttgacca tcgcgtaaac ttccttgttg gtcaggggga tttcctgatg
     1321 caaagcgccg tcctgcggag cgcgcggagc ggaagctagg gccacggggg cgaggtggaa
     1381 caggaagagg gaagcgagag caagagcgac tatcttctgc atggggattg ccttcctttg
     1441 ggacgtgagt atcgggacgt gtcgggcaag gcagggtaca gcggagatgg gccgcgcaca
     1501 tcggacaaaa caacaattct cgcgcggaaa atccacctaa gccgcggggc acaattgtgc
     1561 cccaaacctc gcccggcgcg gaggcccttc gtgagatgca acaggtgttg caacccgggt
     1621 cttcatgggg tatggctccg cccagcactt cggccagacg gcggtatccg acaagtgtgt
     1681 caacggcggg ggcttccacg gcttcggagg tggggggcaa ggtctttcgc ggccttgccc
     1741 ttcgtctttg agcggggata tgatgccggg cctcaagtga atcccacgct ttaacggatc
     1801 gcggtcgagg aggagcatcg aattctttct ctccccgtgt ccgcgcgcga ccgtctcgtg
     1861 cgtggtcgtg gtttcgcggg aggagggccg ggggggtgcg ttggaatgcg aattgcgcta
     1921 cccttccggg gacatttcct cgtggccgaa atcgttacgc atcagcggaa gaaggaggag
     1981 taaatccatg ggtctgttcg acaggatgtt cggtcgcaaa gcgaccgagg tggagaagca
     2041 gccggacgcg cagcagcgct tcgaccagct caaacagaaa taccagtcgg tgttgaacac
     2101 cgccgaccaa caacaaatcc agttccacaa cctgcacgtc gagggcgaca agctctacat
     2161 caagggcgtc gcgccctcgg acgacgcgaa gaacaagttc tgggatcaga tcaagctcgt
     2221 caacccgaac gccgacgaca tttcggccga catcagcgtc tcggcgcaga gcgcgcaggg
     2281 cgctgcggtg ggcggcgggc agggcgcggg cggccagagc tacacggtca agtcgggcga
     2341 cacgctctcg aagatcagca aacagttcta cggcgactcg aacgagtaca tgcgcatctt
     2401 ctacgccaac cgcggccagt tgagcgaccc cgacaaaata caggtcgggc aggagttgac
     2461 catcccgccg gacgacaacg cgtaaggcgc cctcgggcgc ggcggcacag tcgagtggtt
     2521 gcgaaaacga agggcggcgg cgagcttgca agctcaccgc cgccctttcg cgtccgcgtc
     2581 ttcccgcccg cacggcaaag aaaatcggaa ggggccgtgc gactccgctt cgtcggcgac
     2641 ggccccttcc cgcgcagcca cagtgaggga actcaaaggc gggtttgaga cggagagtaa
     2701 agcgccgagg ctagcgaagg tttacgcgcg acttaaatcc gagttaaaaa acgcgcgcac
     2761 gtttccggcc ccgcctcact gtccgaagcg gcggcggtac tcgatggatt cgatgaaggc
     2821 ccgcaccagc tcggcctcga cgtagttgcc gttgaagtcg ttgagcttcg taagccagaa
     2881 gttgaagcct tcgaagttcg ggtcgggggt gccctgaaag tccgggtcgt ccgggttgcg
     2941 cctgagataa ccgaagtatt gcatcaggac gaaggccggg ttcaactcgt tgcgggcgag
     3001 gtctgcgtct tcggccacgc ggcgcaagac ctgcgcgcgc gtcaacgcgc cggttgccag
     3061 agagttgacg atttggtcac gctcggcttg cgagagcgcg ccgcccgcgt tcgcgttcag
     3121 ggcatcgacg aactcggcgg gcgagagcgt cgcggggtaa cgcgaggcga agcgcgtgcg
     3181 cgcggagagg tcgccgacga aggcgttctt gttggcttcg agctgcgcct gccagtcgcc
     3241 gacgccgacc acgacgccgc gcccgaccgt ttgcgcctcg ggcaggaact cctgaatcgt
     3301 gacgggcacg ggcttgcccg cgaggtcgcc gaaggccgcc tgatagacgc ggtagacgaa
     3361 gtagcccgtc tcctgaaact cgatggagag gaaaaaggcc gcggaagtgt ccacacgttt
     3421 gaccgcgcgg cactgaaggt ctgcgccgca cgactcgatg cctcccaccc agaaggcgag
     3481 gccgcccgcg tccgcgccgc ggttcaggaa atcgaggtag tgctggcgga cgaagaagtc
     3541 cgagccgtcg atggggttcg gaacttgcgt gaagggcggg tacttggtct cggagttgat
     3601 ggcgttccgg tcgcactcgg tcagcttgag cggcatgttg ttcgcgccgt cgttgatgtt
     3661 gcagatgtag ttcatgacgg tcgcgccgtc gggctgggcg cagatgtcgc cgttcgggac
     3721 gaccgggtgg tcgaggccca tcgtgtggcc catctcgtgg agcgtgagct tgatgatgaa
     3781 ggtgtcgtag ccgggccgcg aagggtcggc gatgagcgtc gaggtgcccg tgaaggtggc
     3841 gttcgggtcg taggtgagcg tggcggcgac gaccgtgccg tcggcgctga agctcttgtt
     3901 ggcgaaggcc ggcgtgc
//
LOCUS       DBNGOH010000531         4806 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4477324, whole genome shotgun sequence.
ACCESSION   DBNGOH010000531 DBNGOH010000000
VERSION     DBNGOH010000531.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4806)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4806)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4806
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4477324"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1833)
                     /locus_tag="AC159P_10340"
     CDS             complement(<1..1833)
                     /locus_tag="AC159P_10340"
                     /inference="COORDINATES: protein motif:HMM:NF009381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HIR3695889.1"
                     /translation="MKSYPTNQIRNLAVIGHGDAGKTQLTSSLLYVAGATPRWGKVDE
                     GTTTTDYDEDSIARKVTLNTALAHLEWRDHKVNLIDTPGYAAFVSHARPALRAADCAL
                     VVVHAVKGVEVQTEKTWAYANEFITPRIMVVNQMDKEHADFGTALDSARHTFSRAIVP
                     FTLPIGKEHHFRGVVDIVHMKAYEFDANGKAKEIDIPAEGREIVDKERERLVEVIAES
                     DDKLMEKYFELGTLPEEDVVPNISRAIAASRLCPVFAVSSATLVGVSTLLDGILDYAP
                     APDTHEAEHGKVSADGDGGEAVTRGYSPEEPFSAYVFRTIADPFAGRINVMKVVSGKI
                     KSDAAVQNTTRGVAERLGALHSMQGKQLDKIDEASAGDIIACVKLKETQTGDTLSDKA
                     APVVYDPIEYPEAAISFAIEPKSRQDEEKISFALHKILEEDPALRFTRDSQTKEFLLS
                     GSGQLHVETVVEKLRNRYHVEVALHPPKVPYKETITARAEVQGRHKKQTGGRGQFGDC
                     KVVFEPLPRGEGFVFEDKIVGGSVPQQFRPAIEKGIVEAASHGAIAGYPLVDFKATLI
                     DGSFHSVDSDEHSFRAAGRKAFRNAVEKVKPTLLEPVMEVEVVAP"
     gene            2231..2569
                     /locus_tag="AC159P_10345"
     CDS             2231..2569
                     /locus_tag="AC159P_10345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695890.1"
                     /translation="MRFDIRTLMRTISALAIAALLLVGSLAPVMAQGRGRGRGRDNDR
                     RHYGWERGRHRGWSHSARRGRMWRDDDNNNRRWRRRALLRRNRREARRDRWRDGRTFR
                     RTTYRRDRRF"
     gene            2781..3614
                     /locus_tag="AC159P_10350"
     CDS             2781..3614
                     /locus_tag="AC159P_10350"
                     /inference="COORDINATES: protein motif:HMM:NF013324.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HIR3695891.1"
                     /translation="MSNGKYGIAGAALLAVVALIAVGVVYANINYTQEGTVKVVTYFG
                     RIEKVYGPQDGWFTTLAPGREAYEVNIKSFTEMAPVRVTSRDNAALQVEIAVTAFTDA
                     GKIEEYVRKYGFVEQQRHERRNQILHGLIQTEARNAFSEYGAYEIYANQENIQKRIFE
                     ALKPQLANQLQLTTESVQIGNPDFLDDRIEAAASGVVANEKQKQAEEARLEAAKIAAQ
                     TKQIEALTYANPALLDIKKLELQLEIEKARADGIKNHQGALTIMYGGNPGVQVQVPGG
                     R"
     gene            3621..3773
                     /locus_tag="AC159P_10355"
     CDS             3621..3773
                     /locus_tag="AC159P_10355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695892.1"
                     /translation="MRFLVFVFVAVELLQVAVIAMLVVGYRNLQRKVEGDFRFAERPP
                     RSVTED"
     gene            complement(3826..3996)
                     /locus_tag="AC159P_10360"
     CDS             complement(3826..3996)
                     /locus_tag="AC159P_10360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695893.1"
                     /translation="MPVPTRPARRAPFRLHRERRPTEAEGFGGFALTKYDGTTAAVRP
                     EAINHRDTEAQL"
     gene            4183..>4806
                     /locus_tag="AC159P_10365"
     CDS             4183..>4806
                     /locus_tag="AC159P_10365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vanadium-dependent haloperoxidase"
                     /protein_id="HIR3695894.1"
                     /translation="MNKQPSRGLVVPALILLVLGLAVPAWPQGAARRAEKVWGPRYEA
                     SSAQPDRPRHAPPHDTLAQIVHWNEVAVNSSGLDHTPVPVGDPRIFGEQLGPGRSARA
                     VAIVHIAIFEAVNAIKGGYQSYVGVPRANPSASVDAAISQAAHDTLNSMFPSQAAACT
                     ALLVEDLAHIKDGKQKEAGIAVGQQAAAATLVLRAGDGALHAEPLYGI"
BASE COUNT          833 a         1521 c         1610 g          842 t
ORIGIN      
        1 cggggcgacg acttcgactt ccatgacggg ttcgaggagc gtcggcttga ccttctcgac
       61 ggcgttgcgg aaggccttgc gtccggcggc gcggaaacta tgctcgtcgg aatcgaccga
      121 atggaaagag ccgtcgatga gcgtggcttt gaaatcgacg agcgggtagc cggcgatggc
      181 gccgtgcgag gcggcctcga cgatgccctt ctcgatggcg gggcggaact gctgggggac
      241 ggagccgccg acgattttgt cctcgaagac gaagccctcg ccgcgtggca gaggctcgaa
      301 gacgaccttg cagtcgccga actggccgcg cccgccggtc tgcttcttgt ggcgcccctg
      361 cacctccgcg cgcgccgtga tggtctcctt gtaggggact ttcggcgggt ggagcgcgac
      421 ctcgacgtgg tagcggttcc tgagtttctc gaccacggtc tcgacgtgga gctggccgga
      481 gccggagagc aggaactctt tggtctgcga gtcgcgcgtg aagcgcaagg cggggtcttc
      541 ttcgagaatc ttgtggagcg cgaagctgat cttctcctcg tcctgccgcg acttcggctc
      601 gatggcgaag gagatggcgg cctccgggta ttcgatgggg tcgtagacga cgggcgcggc
      661 cttgtccgag agcgtgtcgc cggtctgcgt ctccttgagc ttgacgcagg cgatgatgtc
      721 tccggcgctc gcctcgtcga tcttgtcgag ctgcttgccc tgcatcgagt gcaaggcgcc
      781 gagccgttcg gcgacgccgc gcgtggtgtt ctggacggcg gcgtcggact tgatcttgcc
      841 gctgacgacc ttcatcacgt tgatgcgacc ggcgaacggg tcggcgatgg tgcggaagac
      901 gtaggccgag aaaggctcct cggggctgta gccgcgcgtg acggcctcgc cgccgtcgcc
      961 gtccgcgctg accttgccgt gctcggcctc gtgcgtgtcg ggcgcgggcg cgtagtcgag
     1021 gatgccgtcg agcagagtgg agacgccgac gagcgtcgcc gacgagacgg cgaagaccgg
     1081 gcacaggcgg ctcgccgcaa tcgcgcggct gatgttgggc accacgtcct cctcgggcag
     1141 cgtgcccagc tcgaagtatt tctccatcag cttgtcgtcg gactcggcga tgacttcgac
     1201 cagccgctcg cgctccttat cgacgatctc gcgcccctcg gcggggatgt ctatctcttt
     1261 ggccttgccg ttcgcgtcga actcgtaagc cttcatgtgg acgatgtcca cgacgccgcg
     1321 gaagtggtgc tccttgccga tgggcagggt gaaggggacg atggcgcggc tgaaggtgtg
     1381 gcgggccgag tcgagcgccg tgccgaagtc ggcgtgctcc ttgtccatct ggttgacgac
     1441 catgatgcgc ggcgtgatga actcgttggc gtaggcccag gtcttctcgg tctgcacctc
     1501 gacgcccttg acggcgtgga cgacgacgag cgcgcagtcg gcagcgcgaa gggcggggcg
     1561 cgcgtgcgag acgaaggcgg cgtagcccgg cgtgtcgatg agattcacct tgtggtcgcg
     1621 ccattcgagg tgcgcgagcg ccgtgttgag cgtgaccttg cgcgcgatgg agtcctcgtc
     1681 gtagtcggtc gtggtcgtgc cctcgtccac cttgccccag cgcggcgtgg cccccgcgac
     1741 gtagaggagc gaactggtga gctgcgtctt gcccgcgtcg ccgtgaccga tgacggcgag
     1801 gttgcgaatc tggttcgtgg gataggattt catggcttca ggtcagacat cctttcgaga
     1861 ggtcggcgcg tgcgcgcggc atgacagaca gggaaagacg gcccgcgtcg gcctgagttt
     1921 gaaatttgct gcgggaaata acttcaaggt tgagcgaata atttacgcga ccgaaaggcg
     1981 gaaggcaagc cgccgcttcg cggaaggcaa atggcaaaag gcaagaggca agaggcaaaa
     2041 tcggaaggct aaaaacgtga atccgcgcgc agcgcgtccc cacttttgcc ctttgccttt
     2101 tgccttttgc cttttgcctt ccgtcctccg gcaccacgtt tgctgaaaaa cgggcagaaa
     2161 gtttcggcca cgcgcgaaaa gtcgtggtaa ctctctccct tggcaaactt aaacgtcctg
     2221 gaggctggag atgagattcg atattcgtac actgatgcgg acgataagcg cactcgccat
     2281 cgcggccctg ctgctcgtcg gctcgctcgc cccggtgatg gcgcaggggc gcggcagggg
     2341 gaggggaaga gataacgacc gccgccacta cggctgggag cgtggccgtc atcgcggctg
     2401 gtcgcatagc gcgcgccgcg ggcgcatgtg gcgcgacgac gacaacaaca accggcgctg
     2461 gcgccgccgc gcgctgctcc gccgcaaccg gcgcgaggcg cggcgcgacc gttggcgcga
     2521 tggtcggact ttccgccgca ccacctaccg gcgcgaccgg cggttctaac agctcaaccc
     2581 cagcaggtga aggcgggcgc gacctcattc gcgcccgcct cgattttcag gcccacgcct
     2641 cccgcgcccc gcttcccacg aacaaataaa ctctgccgcg acttcctgcg tataatgtcg
     2701 tgaatatgaa gaagaataaa tggctcgaga tgaagaaaga taaatggccc gaagtattga
     2761 ttccgaataa aggagaatcc atgtcgaatg gcaagtacgg cattgcgggc gcggcgctgt
     2821 tggccgtcgt cgcgctgatc gcggtcgggg tggtctacgc caacatcaac tacacgcagg
     2881 agggcacggt caaggtcgtc acctatttcg gtcgcatcga gaaggtctac gggccgcagg
     2941 acggctggtt cacgacgctg gcgccggggc gcgaggcata cgaggtgaac atcaagtcgt
     3001 tcaccgagat ggcgccggtg cgcgtgacga gcagggacaa cgccgcgctt caggtcgaga
     3061 tcgccgtgac ggccttcacc gacgcgggca agatcgagga gtacgtgcgc aagtacggct
     3121 tcgtcgagca gcagcggcac gagcgccgca accaaatcct ccacgggctg atccagaccg
     3181 aggcgcgcaa cgccttctcc gagtacggcg cctacgagat ttacgccaac caggagaaca
     3241 tccagaagcg catcttcgag gccctcaagc cgcagctcgc gaaccagctc cagctcacca
     3301 ccgagtccgt gcagatcggc aacccggact tcctcgacga ccgcatcgag gccgcggcct
     3361 cgggcgtcgt cgccaacgag aagcagaagc aggccgagga ggcgcggctc gaagccgcca
     3421 agatcgctgc gcagaccaag cagatcgagg cgctgaccta tgccaacccg gcgctgctcg
     3481 acatcaagaa gctcgaactg caactcgaaa tcgagaaggc gcgcgccgac ggcatcaaga
     3541 accatcaggg cgcgctgacc atcatgtacg gcggcaaccc cggcgtgcag gtgcaggttc
     3601 ccggggggag gtgaacgacg ttgagattcc tcgtgttcgt gttcgtcgca gtagagctgt
     3661 tgcaggtcgc cgtgatcgcg atgctcgtcg tcggctaccg caacttgcag cgcaaggtcg
     3721 aaggcgactt ccgtttcgcc gagcgcccgc cgcgctccgt gacggaggat tgaaggactt
     3781 caagaatcaa ccacagagac acggaggcgc agctttgaag accgattaaa gctgcgcctc
     3841 cgtgtctctg tggttgattg cttccggtcg gacggcggcg gtcgtgccgt cgtacttcgt
     3901 gagcgcgaag ccgccgaagc cttcggcttc ggtcgggcgc cgctccctgt gcagtcggaa
     3961 aggcgcccgc ctcgccgggc gtgtcggaac cggcaccgca ttgcgcgtct attgacttgg
     4021 cggcgggttt tggcatagct tctactccct tgtcagtggg ctggaatccc ttgcggccgt
     4081 gctccccacc gcgccggcag tttcagtctc tctaccgcac ctatgtttga aacaccacgg
     4141 taggttctta ccgagccgtc aacccccttg agaggagtac ctatgaacaa gcaaccgagt
     4201 cgtgggctgg tcgtcccggc gttgatctta ctcgtcctcg gcctggccgt ccccgcctgg
     4261 ccgcagggag ccgcacgccg cgccgaaaag gtgtgggggc cgcgttacga ggcttcgtcg
     4321 gctcagcctg accgcccgag acacgccccg ccgcatgaca cgctggccca gatcgtccac
     4381 tggaacgaag tcgcggtcaa ctccagcggc ctcgaccaca cccccgtgcc cgtcggcgac
     4441 ccgcgcatct tcggcgaaca gctcgggccg ggccgctcgg cccgcgccgt cgccatcgtc
     4501 catatcgcca tcttcgaggc cgtcaacgcg atcaagggtg gctaccagag ttacgtcggc
     4561 gtcccgcgcg ccaacccgag cgcctcggtg gacgccgcca tctcgcaggc ggcgcacgac
     4621 acgttgaact cgatgttccc ctcgcaggcc gcggcctgca ccgccctgct cgtcgaagac
     4681 ctcgcccaca tcaaggacgg gaagcagaag gaggccggca tcgccgtcgg ccagcaggcg
     4741 gccgcggcga ccctcgtgct gcgggccggc gacggcgcgc tgcacgccga gccactctac
     4801 ggcatt
//
LOCUS       DBNGOH010000532         3952 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1729284, whole genome shotgun sequence.
ACCESSION   DBNGOH010000532 DBNGOH010000000
VERSION     DBNGOH010000532.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3952)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3952)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3952
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1729284"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..971)
                     /locus_tag="AC159P_10370"
     CDS             complement(<1..971)
                     /locus_tag="AC159P_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008462048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HIR3695895.1"
                     /translation="MGFSNLFRTKDVAQIKRDAELGYGDHGELEVEDRHLRKTLGVVD
                     LTAFGIAAIIGAGIFSTIGTAAFNGGPAVAFLFVFTAIACAFSALCYAEFAAQIPVAG
                     SAYTYAYASFGELIAWIIGWDLLIEYAIGNIAVAISWSDYFTGLLASYNINIPAYLTM
                     DFLSASRGFEALNALTAEGKLADITGSMKTAHEAWTTAPRVAGIPVVCDLPALFIVLV
                     ITVLVYIGIRESKRASNIMVAVKVAVVLMVIVVGATYVNTENWSPFAPNGIGGVLKGV
                     SAVFFAYIGFDAITTTAEECKNPQRDLPLGMIFSLIICTVLYIAIALV"
     gene            complement(1318..1695)
                     /locus_tag="AC159P_10375"
     CDS             complement(1318..1695)
                     /locus_tag="AC159P_10375"
                     /inference="COORDINATES: protein motif:HMM:NF012790.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HIR3695896.1"
                     /translation="MWRKFNHWLALILCAALASLAAACKAGDGAGASRAGAGAASTTT
                     STTTAGATPHPTSTPNDGVRRVTTDEARALVERGEALIVDVRVKGDYDKGHIKGSICV
                     PRTEIDKRLAELPKDKLLIFYCA"
     gene            2026..3342
                     /locus_tag="AC159P_10380"
     CDS             2026..3342
                     /locus_tag="AC159P_10380"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3695897.1"
                     /translation="MRAKEEVTKVSIEGEGVPVAAPPDGRAPRSEPNGEAAAGVRGIS
                     KSFKGTRVLEDVSFDVAEGEALVLLGASGSGKTTILRIIAGLEQPDGGSVVLHGKDVT
                     ALPARERGVGVIFQSYALFPRMTVEKNIAYGLRIRKRPRKEIRETVERLLKLVRLEEH
                     RKKRPAQLSGGQQQRVAIARTLAYNPQVLLFDEPFGALDAQTRVRLRREIRALLRKVN
                     VPAVFITHDQEEALELGDRIAVLHAGRLEQIGTPDEVYNKPETEYVATFLGAANLLVG
                     VYSRGHVHVGGAVIPADGESARFREGQSVKLVFRPEDVHLGLGGALPEGCSPLASGVV
                     EEKEFAGAYERLTVRLDLAARRAEEGDSDASPLYAGANSTAPANASATATSTGTPVFV
                     TRPKPEASAAPLRPGDRVAVGLTAFRVLPNYALASERASRVVEVEK"
     gene            complement(3519..>3952)
                     /locus_tag="AC159P_10385"
     CDS             complement(3519..>3952)
                     /locus_tag="AC159P_10385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695898.1"
                     /translation="AITEYTEDDLPHKTNEDRFADFASRWAIRFTSRATNTLLGGGVY
                     PVLFKQDPRYRPSESKNIFKRALHAASRVFVTDDDDGNLEPNVSRWAGSLTSSAIANL
                     YEQSTPGRDRIGTDATFRRFGSSFTSGMVNNIIREFLGDLF"
BASE COUNT          687 a         1247 c         1385 g          633 t
ORIGIN      
        1 acgagcgcga tggcgatgta gagcaccgtg cagatgatga gcgagaagat catcccgagc
       61 ggcaggtcgc gctgcgggtt cttgcactcc tcggcggtgg tcgtgatggc gtcgaagccg
      121 atgtaggcga agaagaccgc cgagacgcct ttcaagacgc cgccgatgcc gttcggcgcg
      181 aacggcgacc agttctcggt gttgacgtag gtggcgccga ccacgatcac catcagcacg
      241 acggcgacct tcaccgcgac catgatgttc gacgcgcgct tcgactcgcg gatgccgatg
      301 tagacgagca cggtgatgac gaggacgatg aagagcgcgg gcaggtcgca gacgaccggg
      361 atgccggcga cgcgcggcgc cgtcgtccac gcctcgtggg ccgtcttcat gctgccggtg
      421 atgtcggcga gcttgccctc ggcggtgagc gcgttcagcg cctcgaagcc gcgcgaggcc
      481 gaaagaaagt ccatcgtcag gtaggccggg atgttgatgt tgtagctggc gagcagcccc
      541 gtgaagtagt cgctccaaga gatggcgacg gcgatgttgc cgatggcgta ctcgatgagc
      601 aagtcccagc cgatgatcca cgcgatcagc tcgccgaacg aggcgtaggc gtaggtgtag
      661 gcggagccgg cgaccggtat ctgcgcggcg aactcggcgt agcagagcgc ggagaaggcg
      721 caggcgatgg ccgtgaagac gaagaggaag gcgaccgccg ggccgccgtt gaaggcggcg
      781 gtgccgatgg tcgagaagat gccggcgccg atgatggcgg cgatgccgaa cgccgtcaga
      841 tcgacgacgc cgagcgtctt ccggaggtgc ctgtcttcga cctccagctc gccgtggtcg
      901 ccgtagccga gttcggcgtc gcgcttgatc tgggcgacat ccttggtgcg gaagaggttc
      961 gagaagccca tcctgataga aagtctccgc tgcctatatt gttagagctt gggactgcct
     1021 gaacgaggtc gcgccggttt gtcttcgcgg tcggcggagt ttgtcatagg cgaaggcggg
     1081 aaggcaacaa aaaaggggcg aaaaggggaa gagggaaaaa ggcgaaaagg gagaagacgg
     1141 tcttcctcct tttcgccctt tcccctttta accttttccc ctcagttctt atttcgcggg
     1201 ttcggtcggc agccccctgc ccgtccaggc ggcccagccg ccgagcagtg ccgccgtgtt
     1261 ttcgtagccg cgcttgcgga tgtcgagcgc cgccccggcg ctctggtgtt cggtcggtca
     1321 agcacagtag aagatgagga gcttgtcctt cggcagctcc gcgagccgct tgtcgatctc
     1381 tgtgcgcggg acgcagatcg aacctttgat gtggcccttg tcgtagtcgc ctttcacgcg
     1441 cacgtccacg ataagggcct cgccgcgctc gaccagcgcg cgcgcctcgt cggtggtgac
     1501 gcggcgcacg ccgtcgttcg gcgtcgaggt cgggtgcggg gtcgcgcccg ccgtcgtcgt
     1561 cgaggtcgtc gtcgaggccg cgcccgcgcc cgcgcgcgag gcgcccgcgc cgtcgccggc
     1621 cttgcacgcc gcggcgagcg acgcgagggc cgcgcagaga atcaacgcca gccagtgatt
     1681 gaacttccgc cacatctcga acggcctccg cttgaagcta aggatagaga gttgaggtgc
     1741 cgaccgggga cacggcggct agcatcgcac acaacgcggc ccgaatggaa gcgcggcggc
     1801 aaggcttccc gaaacggcgc cgcctgtgct atagaagcgc catcatttcg taatccgggg
     1861 ccggaagtcg cgggcgccga cgggcgcggc atggagaaga ctacggaaaa gccgtgaacc
     1921 gtgaaccgta aaccgtgaca agtgaagaac tgtttttctc ctgatcacgg tttacggttc
     1981 acggttcacg gttcacggct tttcacggtt cacggcttta tcacgatgcg ggcgaaggaa
     2041 gaagtgacga aagtttcgat tgagggcgag ggcgtcccgg tcgccgcgcc gccggacggg
     2101 cgcgcgccgc ggagcgagcc gaacggcgag gcggcggcgg gcgtgcgagg gataagcaag
     2161 agcttcaagg gcacgcgcgt gctggaggac gtgagcttcg acgtggccga gggcgaggcg
     2221 ctggtgctgc tgggcgcttc ggggagcggg aagaccacca tcctgcgcat catcgcgggg
     2281 ctggagcagc ccgacggcgg cagcgtcgtc ctccacggca aggacgtgac ggcgttgccc
     2341 gcgcgcgagc gcggcgtcgg cgtcatcttc cagtcctacg cgctcttccc gcgcatgacg
     2401 gtcgagaaga acatcgccta cggcctgcgc atccgcaagc gcccgcgcaa ggagatcaga
     2461 gagaccgtcg agcggctgct gaagctcgtc cgcctcgaag agcaccgcaa gaagcggccc
     2521 gcgcagctct cgggcgggca gcagcagcgc gtggcgattg cgcgcacgct cgcctacaac
     2581 ccgcaggtct tgctcttcga cgagccgttc ggcgctctgg acgcgcagac gcgcgtgcgg
     2641 ctgcggcgcg agatacgcgc gctcctgcgc aaggtcaacg tccccgccgt cttcatcacg
     2701 cacgaccagg aggaggcgct cgaactcggc gaccgcatcg ccgtgctgca cgccgggcgg
     2761 ctcgaacaga tcggcacgcc cgacgaggtc tacaacaagc cggagaccga gtacgtcgcc
     2821 accttcctcg gcgcggcgaa tctgctcgtc ggcgtctaca gtcgcggcca cgtgcacgtc
     2881 ggcggcgccg tcatccccgc cgacggcgag tcggcgcgct tccgcgaagg ccagtccgtc
     2941 aaactcgtct tccgccccga agacgtgcac ctcggcttgg gcggcgcgct ccccgaaggg
     3001 tgcagcccgc tcgcctccgg cgtcgtcgaa gagaaagagt tcgccggcgc ctacgaacgc
     3061 ctcaccgtcc gcctcgacct cgccgcccgc cgcgccgaag agggcgacag cgacgcctcg
     3121 cccctctacg cgggcgcgaa ctcgaccgcg cccgccaacg cgagcgcgac ggcgacgagc
     3181 accggcacgc ccgtcttcgt cacgcgcccg aagcccgaag ccagcgccgc gccgctccgc
     3241 cccggcgacc gcgtcgccgt cggcctgacg gccttccgcg tgctccccaa ctacgccctc
     3301 gcctccgaac gcgcgagccg cgtcgtcgag gtggaaaaat agtaggcagc gggcagcagg
     3361 cagcaggcag ttaaagtcaa aagcaggagg cttgggacgt ggcgtctcaa gcctcctgct
     3421 ttgttgcttg aagagagaag aagcttggga ggtaaagtcc caagcctctt cgtcattccg
     3481 tcttaactgc ctactgccta ccgcctactg cctacttctc agaacaggtc gcccaaaaat
     3541 tcgcggatga tgttgttgac catgccggag gtgaaggagg agccgaagcg gcggaaggtg
     3601 gcgtcggtgc cgatgcggtc gcggcccggc gtgctctgct cgtagaggtt ggcgatggcg
     3661 ctcgacgtga gcgagccagc ccagcgcgag acgttgggtt cgaggttgcc gtcgtcgtcg
     3721 tccgtgacga agacgcgcga ggcggcgtgg agcgcgcgct tgaagatgtt cttgctctcc
     3781 gaagggcggt agcgcgggtc ttgtttgaag aggacggggt agacgccgcc gcccaagagc
     3841 gtgttcgtcg cgcgcgaggt gaaacggatg gcccagcgcg aggcgaagtc cgcgaagcgg
     3901 tcttcgttcg tcttgtgcgg cagatcgtct tcggtgtact cggtgatggc gg
//
LOCUS       DBNGOH010000533         2088 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4111229, whole genome shotgun sequence.
ACCESSION   DBNGOH010000533 DBNGOH010000000
VERSION     DBNGOH010000533.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2088)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4111229"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..482
                     /locus_tag="AC159P_10390"
     CDS             <1..482
                     /locus_tag="AC159P_10390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3695899.1"
                     /translation="GARLKWAQRARALAEGRAFIMWTFEPLRARNAHYNLVRLGVEVR
                     TYEVNYYGTDNATQSAEQGAHQGLDSDRLVAEWDLRAARVEALARRADAPAPGEPAAT
                     VVIPPDWSALVREDAAAARDDLFRVRTEFRQHLSRGLVCAGFERDATRPRYLFYREP"
     gene            588..2000
                     /locus_tag="AC159P_10395"
     CDS             588..2000
                     /locus_tag="AC159P_10395"
                     /inference="COORDINATES: protein motif:HMM:NF013696.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HIR3695900.1"
                     /translation="MPFTRYDRYVDRHARTFTERLQALCRMPSVAARGTGMRAVAEMV
                     EQMLQRTGAGTRIFRVGSGYPIVYGECGAGERRYLVYGHYDVQPVGQLTEWSSGPFAA
                     AIVDGRLYARGASNSKGDLVARVAAIEAYQKTFGKLPVCFRFLVEGEDGLASPSLYRF
                     AAENAALLVADGCIWDDGYRDTGGRLVINLGFKGIVFLELRAYGARTDLHSKWGGIVP
                     NPAWRLVQALTTIVSPKGVITIDGFSSHVAPITNEDKQYLKTVELDEAGLRREFRIGG
                     WVRGMTGRELTKELIFGPTCTICGIRTGHTDAGAKTVLPGTALARLDFRLVPDLTPEL
                     VVRLLRKHLDTRGFQDIEIVELGGEPYAKSSPRSLVARAAIETAEGLYGQPPVVYPLD
                     PASGPVGIVCGVQDPPTPVVSFGVSHSGSNPHGPDENIRIEDFVHGIKYFGRVIHRLA
                     EIETEVAAPPESEAVARSNR"
BASE COUNT          327 a          746 c          694 g          321 t
ORIGIN      
        1 tcggcgcgcg tctgaagtgg gcgcaaagag cgcgcgcgct ggccgagggc cgcgccttca
       61 tcatgtggac gttcgagccg ctgcgcgcgc gcaacgcgca ctacaacctc gtccgcctcg
      121 gcgtcgaggt gcgcacctac gaagtgaact actacggcac cgacaacgcc acgcagtccg
      181 ccgagcaggg cgcgcatcag ggtctcgaca gcgaccgcct cgtggccgaa tgggacttgc
      241 gcgcggcgcg cgtcgaagcg ctcgcgcgcc gcgcggacgc gcccgccccc ggcgaacccg
      301 cggccacggt cgtcatcccc cccgactggt ctgcgctcgt ccgcgaagac gccgccgccg
      361 cgcgcgacga cctgttccgc gtccgcaccg agttccggca acacctctcg cgcggactcg
      421 tctgcgcggg cttcgagcgc gacgcgacgc gcccgcgata ccttttttat cgtgaaccgt
      481 gaaccgtgaa ccgtgacaag ttaagagcag tatttcgtca cgttcatggt tcccgcttca
      541 cgggttttct tatcacggtt cacggtttac ggcttacggc tttcgacatg cctttcaccc
      601 gctacgaccg ctacgttgac cgccacgcgc gcaccttcac ggagcgtttg caggcgttgt
      661 gccggatgcc gtcggtggcg gcgcgcggga cggggatgcg ggcggtggcc gagatggtcg
      721 agcagatgtt gcagcgcacg ggcgcgggca cgcgcatctt ccgcgtcggg agcggctacc
      781 ccatcgtgta cggcgagtgc ggggcgggcg agcggcgcta cctcgtctac ggccactacg
      841 acgtgcagcc cgtggggcag ttgaccgagt ggagttcggg gccgttcgcg gcggccatcg
      901 tggacggcag gctctacgcg cgcggcgcgt cgaacagcaa gggcgatctg gtggcgcgcg
      961 tcgccgccat cgaggcttat cagaagacgt tcggcaagct cccggtctgc ttccgcttcc
     1021 tcgtcgaggg cgaggacggg ctggccagtc cgagcctcta ccgcttcgcc gccgagaacg
     1081 ccgcgctgct cgtcgcagac ggctgcatct gggacgacgg ctaccgcgac acgggcgggc
     1141 ggctcgtcat caacctcggc ttcaagggca tcgtcttcct tgagctgcgc gcgtacggcg
     1201 cgcgcaccga cctgcattcg aagtggggcg gcatcgtccc gaacccggcg tggcggctcg
     1261 tgcaggcgct cacaacaatc gtctcgccca aaggggtcat caccatcgac ggcttctctt
     1321 cgcacgtcgc gccgatcacg aacgaggaca agcagtacct gaagacggtc gagctggacg
     1381 aggcgggcct gcgccgcgag ttccgcatcg gcggctgggt gcgcggcatg acgggccgcg
     1441 agctgacgaa ggagctgatc ttcggcccga cctgtaccat ctgcggcatc cgtacgggcc
     1501 acaccgacgc gggcgccaag acggtactgc ccggcacggc gctcgcgcgc ctcgacttcc
     1561 gcctcgtgcc cgacctcacg cccgagctgg tcgtccggct gctgcgcaag cacctcgaca
     1621 cgcgcggctt tcaggacatc gagatcgtcg agctgggcgg cgagccgtac gccaagtcgt
     1681 cgccgcgctc gctggtcgcg cgcgccgcca tcgagaccgc cgagggcctc tacggccagc
     1741 cgcccgtcgt ctacccgctc gacccggcga gcggccccgt cggcatcgtc tgcggcgtgc
     1801 aggacccgcc cacgcccgtc gtctcgttcg gcgtcagcca ctccggctcg aacccccacg
     1861 ggccggacga gaacatccgc atcgaagact tcgtccacgg catcaagtac ttcggtcgcg
     1921 tcatccaccg cctcgccgag atcgagaccg aggtggccgc gccgcccgag agcgaggccg
     1981 tcgcccgctc gaacaggtaa catcaaagga ctgaggactg aggactgagg aaaagccgtg
     2041 aaccgtgaac cgtaaaccgt gacgagtccg accagcaccg ctctcatc
//
LOCUS       DBNGOH010000534         4970 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3744641, whole genome shotgun sequence.
ACCESSION   DBNGOH010000534 DBNGOH010000000
VERSION     DBNGOH010000534.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4970)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4970)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4970
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3744641"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..798)
                     /locus_tag="AC159P_10400"
     CDS             complement(<1..798)
                     /locus_tag="AC159P_10400"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015231628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HIR3695901.1"
                     /translation="MATRHDEDGAALELVADGFSFPTSLTFDDEGGTYVAESGLPFGG
                     ARPGGRVWRLGAGARRMLLVDDLRPPVNGLTFHDGQLYVSEGGHPARISHFRLDGSGQ
                     TVILDNLPGPGNYHTNMVAFAPDGKLYFSQGAMTNTGVIGLDAYELGWLRRLPHLHDL
                     PGYDIVLTGVNVETPNPLADEDNARTQTGAFVPFGQRTEPGRRFAAELPCTAAVMRCN
                     ADGSELELVAWGLRNAYGLGFLPDGRLLAVDQGADDRGSRPVADVPDM"
     gene            complement(832..1368)
                     /locus_tag="AC159P_10405"
     CDS             complement(832..1368)
                     /locus_tag="AC159P_10405"
                     /inference="COORDINATES: protein motif:HMM:NF014689.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0071421 - manganese ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HIR3695902.1"
                     /translation="MTTLLLLGLALGFDSFRVSLGLGGLRLSRARRRLIALSFGVCDG
                     LAPLVGLLLGQSLVATVGRWTEYIAPFALGGYGLYVIYVACRRGESEQEAEGAWVVLG
                     LPLALSLDNFAAGVGLGLLQYPALLSAAIIGSVSGLMSLAGLQLGSALARRLPFDADL
                     AGGVALVVLALAVGLDVL"
     gene            complement(1379..>4970)
                     /locus_tag="AC159P_10410"
     CDS             complement(1379..>4970)
                     /locus_tag="AC159P_10410"
                     /inference="COORDINATES: protein motif:HMM:NF015140.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta strand repeat-containing protein"
                     /protein_id="HIR3695903.1"
                     /translation="GPFLVADFNNDNKPDVFAGSQSFSSSKVMLGNGAGGFASSGTLL
                     IPVHSFADAGDFNGDGKVDLVLTGFGGTFVALVPGDGAGNFAKETFFTVPTAGPVAAA
                     DVNNDGKLDALTAGTNSVVVQLGDGAGGLGPATAFASGHDGAAISVGDVNNDGKKDVA
                     VAGNSSGQRRGTVSVLAGDGAGAFGPPASFRVGLGSSSAVLIGDFNGDGRGDLAGSGV
                     TSDFVVGTAPGAVTIFLGDAAGGLSAPLTFSTGPSPFAIASGDLNGDGRPDLAVTSSQ
                     NNVYVALGNSQSLFGAFTPVGVGARPIGVTLADVNNDGKLDLLSANQDFSTVSVLLGD
                     GAGGFGAATVINSVGSSPNSVKAGDYNLDGRVDLAVTNNFGDVEILRGDGAGGFAPPT
                     RIRLGNFAYNSAAGDFNSDGLPDLAVGFSGGIALLEGTPAGVFAPPVVVPTGASSASA
                     VVAGDFNGDGKTDLAAAHEVTSLIAVLIGDGAGGFAAPKSYTAGTGGRSLVAADFNGD
                     GDQDLATANIRGTVSLLAGDGAGDFAPAVSFLSGGPAPRALTTGDFDGDGSVDIAVAN
                     FGSNAGGGETLNGNVTAVLNNCDALPPALPRLSVVGVTTTLTEGDAGTTGATFNVHLS
                     STSTKPVTVSYYPFSQTAERGADFAGASGRIVFNPGETFKAVNVPVVGDTADEFDETF
                     GLALALPLNAVIDGASASATILDDDPEPSLSVADVAQAEGSGATGAILFNVTLSAASG
                     KPITVAYAAADGTAQAGADYQNASGVVTFNPGETSKAVAVNVFADSAIEADESFFLNL
                     SNPTNAGLADAQGAATLRNDDGTVQFAAATFNATEGVEPSVFVTVARTGETSGVSIIG
                     YATFDGTASERRDYNAALGRLRFAPGETTKTFEVVITDDRFAEGAETLTVSLSGAVGA
                     EIGTQSSATVNINSDDAAGGPSPVKDASFDTEFFVRQHYADFLSRVPDAGGLAFWVGG
                     IESCGADQQCREVKKIDTSAAFFLSIEFQEAGFLAYRAHKAAFGSLPNTPGVPLRLRD
                     FLADSRRIADGVVVGAPGWPEQLEANKQAYFAELVTRAGFVALNPPTLNAVQYVNGLF
                     ARGGVLPTFAERQAAIAAFGAGGTAGRAAALRSVAESQTLKQAETNRAFVLMQYFGYL
                     RRNPDDPQDTNFDGFNFWLTKLNQFGGNYVDAEMVKAFIQSIEYGDRFGH"
BASE COUNT          736 a         1665 c         1861 g          708 t
ORIGIN      
        1 catgtcgggc acgtccgcga cggggcggct gccgcggtcg tccgcgccct gatcgaccgc
       61 gagcagccgc ccgtccggca ggaagcccag cccgtacgcg ttccgcaaac cccacgcgac
      121 cagctccagt tcgctcccgt ccgcgttgca gcgcatgacg gcggcggtgc agggcaactc
      181 cgcggcgaag cgtcggcccg gctccgtgcg ctggccgaag gggacgaagg cgccggtctg
      241 tgtgcgcgca ttgtcttcat cggcgagcgg gttcggcgtc tcgacgttga cgcccgtgag
      301 cacgatgtcg tagcccggca ggtcgtgcag gtgcggcagg cggcgcagcc agccgagttc
      361 gtacgcgtcg aggccgatga cgcccgtgtt ggtcatcgcg ccctgactga agtagagctt
      421 gccgtcgggc gcgaaggcga ccatgttcgt gtggtagttg ccggggccgg gcaggttgtc
      481 gaggatgacg gtctgcccgc tcccgtcaag acggaagtgg ctgatgcgcg cggggtgccc
      541 gccctcggag acgtaaagtt gaccgtcgtg gaacgtgagg ccgttgacgg gcgggcgcag
      601 gtcatcgacg agcagcatgc gccgcgcgcc ggcgccgagc cgccacacgc gaccgcccgg
      661 cctcgcgccg ccgaagggca gccccgactc cgccacgtaa gtcccgcctt cgtcgtcgaa
      721 cgtcaagctg gtcgggaacg agaacccgtc ggcgaccaac tcaagcgccg cgccgtcttc
      781 gtcatgtcga gttgccatcg ggaaagttta gcaggtggtc gagaggtgtc gtcaaagaac
      841 gtccagcccg acggcgagcg cgaggacgac gagcgcgacg ccgccggcga ggtctgcgtc
      901 gaaggggagg cggcgcgcga gcgcgctgcc gagttggagg ccggcgagcg acatgaggcc
      961 gctcacggag ccgatgatcg ccgccgagag cagcgccggg tactgtagca gcccgaggcc
     1021 gacgcccgcg gcgaagttgt cgaggctcag cgcgagcggg aggcccaaca cgacccacgc
     1081 gccctcggcc tcctgctccg actcgccgcg gcggcacgcc acgtagatga cgtagaggcc
     1141 gtagccgccg agcgcgaacg gcgcgatgta ttccgtccag cgcccgacgg tcgcgacgag
     1201 cgactgtccg aggagcaggc cgacgagcgg cgccagccca tcgcacacgc cgaaggagag
     1261 cgcgatcagc cgccgccgcg cacggctcag gcgcaggccg cccaagccga gcgagacgcg
     1321 gaagctgtcg aacccgagcg ccagtccgag tagcagcagt gtggtcatcg cgacaagtct
     1381 agtggccgaa gcggtcgccg tactcgatgg actggatgaa cgccttgacc atctcggcgt
     1441 ccacgtagtt gccgccgaac tggttgagct tcgtgagcca gaagttgaag ccgtcgaagt
     1501 tcgtgtcctg cgggtcgtcc gggttacgcc ttaagtaacc gaagtactgc atgagcacga
     1561 acgccctgtt ggtctccgcc tgtttcaggg tctgcgactc ggcgaccgag cggagcgccg
     1621 ccgcgcggcc cgccgtgccg cccgcgccga aggccgcgat ggccgcctgc cgctccgcga
     1681 acgtcggcag gacgcccccg cgcgcgaaca gcccgttcac gtactgcacg gcgttaagcg
     1741 tcggcgggtt gagcgcgacg aacccggcgc gcgtgaccaa ctcggcgaag tacgcctgct
     1801 tgttggcttc gagttgttca ggccagccgg gcgcgccgac gacgacgccg tccgcgatgc
     1861 gccgcgagtc cgcgaggaag tcgcgcagcc gcagcggcac gcccggcgtg ttcggcaggc
     1921 tgccgaacgc ggccttgtgc gcgcggtagg cgaggaagcc cgcctcctgg aactcgatcg
     1981 agaggaagaa ggcggcgctc gtgtcgatct tcttcacctc gcggcactgc tggtcggcgc
     2041 cgcaggactc gatgccgccc acccagaagg cgagtccccc ggcgtccggc acgcggctga
     2101 ggaagtcggc gtagtgctgg cggacgaaga actcggtgtc gaaggaggcg tccttgacgg
     2161 ggctggggcc gcccgccgcg tcgtcgctgt tgatgttgac ggtcgcggag gactgcgtac
     2221 cgatctcggc gccgaccgcg ccggagagcg agacggtgag ggtctccgcg ccttcggcga
     2281 agcggtcgtc ggtgatgacg acctcgaagg tcttcgtcgt ctcgccgggc gcgaagcgga
     2341 ggcggccgag cgcggcgttg tagtccctgc gctcggaggc cgtgccgtcg aaggtggcgt
     2401 agccgatgat ggagacgccc gaagtctcgc cggtgcgcgc gacggtgacg aagacgctcg
     2461 gttctacgcc ctcggtcgcg ttgaaagtcg cggcggcgaa ctgaacggtg ccgtcgtcgt
     2521 tgcgcagcgt cgccgcgccc tgcgcgtcgg ccaggccggc gttcgtcggg ttcgagaggt
     2581 tgaggaagaa ggactcgtcc gcttcgatgg ccgagtccgc gaagacgttg acggcgacgg
     2641 ccttgctcgt ctcgccgggg ttgaaggtga cgacgcccga ggcgttctgg tagtcggcgc
     2701 ccgcctgcgc ggtgccgtcg gcggcggcgt aggcgacggt gatcggcttg ccgctcgcgg
     2761 cggagagcgt gacgttgaag agaatcgcgc ccgtcgcgcc gctgccctct gcctgcgcca
     2821 cgtcggcgac ggagagcgaa ggctccgggt cgtcgtcgag gatggtggcg gacgcggagg
     2881 cgccgtcgat gacggcgttg agcggcagcg cgagcgcaag cccgaacgtc tcgtcgaact
     2941 cgtccgcggt gtcgccgacg acgggcacgt tgacggcctt gaacgtctcg ccggggttga
     3001 agacgatcct gccggaagcg cccgcgaagt cggcgcccct ctcggcggtc tgcgagaaag
     3061 ggtaataact gacggtcacg ggcttcgtgc tcgtcgaaga gaggtggacg ttgaaggtcg
     3121 cgccggtcgt gcccgcgtcg ccttcggtga gcgtcgtcgt cacgcccacg acggagaggc
     3181 gcgggagcgc gggcggcagc gcgtcgcagt tgttgaggac ggcggtgacg ttgccgttca
     3241 gcgtctcgcc gccccccgcg ttgctgccga agttggcgac cgcgatgtca accgagccgt
     3301 cgccgtcgaa gtcgcccgtg gtgagcgcgc gcggcgcggg gccgccgctc aggaagctga
     3361 cggcgggcgc gaagtcgccc gccccgtcgc ccgcgagcag agagacggtg ccgcgtatgt
     3421 tggcggtggc gaggtcttgg tcgccgtcgc cgttgaagtc cgcggcgacg agcgaccgcc
     3481 cgccggtgcc cgcagtgtaa gacttcggcg cggcgaagcc gccggccccg tcgccgatga
     3541 ggacggcgat gagggacgtg acctcgtgcg ccgcggcgag gtcggtcttg ccgtcgccgt
     3601 tgaaatcgcc cgcgaccacc gcggaggcgg acgacgcgcc ggtgggcacg acgacgggcg
     3661 gggcgaagac gcccgccgga gtcccctcta gcagcgcgat gccgccgctg aagcccacgg
     3721 cgaggtcggg gaggccgtcc gagttgaagt cgcccgccgc cgagttgtac gcgaagttgc
     3781 cgagcctgat gcgggtcggc ggggcgaagc cgcccgcgcc gtcgccgcgc aggatttcga
     3841 cgtcgccgaa gttgttggtg acggcgaggt ctacccgccc gtcgaggttg tagtcgcccg
     3901 ccttgacgga gttcggcgac gagccgacgg agttgatgac ggtcgccgcg ccgaagccgc
     3961 ccgcgccgtc gcctaagagg acggagacgg tgctgaagtc ttggttggcg ctcagcaggt
     4021 cgagcttgcc gtcgttgttc acgtccgcga gcgtgacgcc gatgggccgc gcgccgacgc
     4081 cgacgggcgt gaaggcgccg aagagactct gcgagttgcc gagcgcgacg tagacgttgt
     4141 tctgcgagct ggtgacggcg aggtcgggcc gcccgtcgcc gttgaggtcg cccgacgcta
     4201 tcgcgaaggg ggacggcccg gtgctgaacg tgaggggcgc ggacagcccg ccggcggcgt
     4261 cgccgaggaa gatcgtgacc gcgccgggcg ccgtgccgac gacgaagtca gaagtcacgc
     4321 cggaccctgc caggtcgccc cgcccgtcgc cgttgaagtc gccgatcaag accgcgctcg
     4381 acgaacccag gccgacgcgg aacgaggcgg gcgggccgaa agcccccgcg ccgtcgcccg
     4441 ccagcacgga caccgtgccg cgcctctgcc cgctgctgtt gcccgccacc gccacgtcct
     4501 tcttgccgtc gttgttcacg tcgccgacgc tgatcgcggc cccgtcgtgg cccgacgcga
     4561 aggcggtcgc cggcccgagg ccgcccgcgc cgtcgccgag ctgcacgacg acagagtttg
     4621 tgcccgccgt caacgcgtcg agcttgccgt cgttgttcac gtccgccgcc gcgaccgggc
     4681 cggcggtcgg gacggtgaag aaagtctcct tcgcgaagtt ccccgcgccg tcgcccggca
     4741 cgagcgcgac gaaggtgccg ccgaagcccg tcagcacgag gtcaaccttg ccgtcgccgt
     4801 tgaagtcgcc cgcgtcggcg aagctgtgca cggggatgag cagcgtgccc gacgaggcga
     4861 agccgcccgc gccgttgccg agcatcacct tcgacgagct gaagctctgg ctgccggcga
     4921 acacgtcggg cttgttgtcg ttgttgaagt ccgcgacgag gaacgggccg
//
LOCUS       DBNGOH010000535         8130 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_996671, whole genome shotgun sequence.
ACCESSION   DBNGOH010000535 DBNGOH010000000
VERSION     DBNGOH010000535.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8130)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8130)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8130
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_996671"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..392)
                     /locus_tag="AC159P_10415"
     CDS             complement(<1..392)
                     /locus_tag="AC159P_10415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HIR3695904.1"
                     /translation="MKQTSKSRAGHARNSSGNSRRSFEERLAQKRARAVEDAKLRAKQ
                     AQGKFRRFVNIRFRKEEVIEALSLRRGECNRCGACCELLFKCPVLKKHEDGSTTCGIY
                     EDRPNQCRLFPIEQRDIEEVRGQCSFYF"
     gene            complement(550..897)
                     /gene="trxA"
                     /locus_tag="AC159P_10420"
     CDS             complement(550..897)
                     /gene="trxA"
                     /locus_tag="AC159P_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HIR3695905.1"
                     /translation="MSEHVKDVSDTSFEADVLKSDRPVLVDFWAQWCAPCRMLAPTVE
                     AVAEKYAGSATVVKLNVDDNPAISQRYGIKGIPTLILFKNGKEEERVVGATSKEAISR
                     MIDKYVGTAASAS"
     gene            complement(1033..2457)
                     /locus_tag="AC159P_10425"
     CDS             complement(1033..2457)
                     /locus_tag="AC159P_10425"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR02095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033840 - alpha-1,4-glucan
                     glucosyltransferase (NDP-glucose donor) activity [Evidence
                     IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase"
                     /protein_id="HIR3695906.1"
                     /translation="MRVAILSSEAVPFAKTGGLGDVAGALPKALQNEGADVVLIHPLH
                     GQTKREHLQEQVFDNLEVEWLGGVRRASVWYSEATGAPSFLINAPEYFMRPDVYGYRD
                     DHERFAFFCRAALALLGRLGPAPDVVHGNDWPCGFAAVWLKAGRPYGGFLSQTRTLFS
                     IHNLAYQGLFDPDDLWRLGFKDGDEQNVFLKDRVASVLKAGLMTSDALSTVSRRYSFE
                     IQTPEHGHGLDWLLRLRRDRLIGITNGVDYDVWNPATDPHLAANYSAEDLTGKRACKL
                     DLLRSFGLPEEPERPVVACVSRLTPQKGFDLVKQAAGRILEAGVFFVALGSGASEYED
                     FLQALHDHAPSRVGIYKGFSEPLAHRIEAGADIFLMPSQYEPCGLNQMYSMRYGTVPV
                     VRATGGLDDTVENFDRARAAGSGFKFGPYSAHAMLEKIYEALYCYRQPEVWRTIQLNG
                     MREDNSWAAAARKYIEVYRAVARM"
     gene            complement(2653..3876)
                     /gene="rho"
                     /locus_tag="AC159P_10430"
     CDS             complement(2653..3876)
                     /gene="rho"
                     /locus_tag="AC159P_10430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020712094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HIR3695907.1"
                     /translation="MSISKLTGIAKDLDVPGATGMRKQELIFKVLAAQTEKSGLIFSE
                     GVLETLPDGFGFLRAPEYNYLPGPDDIYVSPSQIRKFDLRTGDTVSGQIRPPKEGERY
                     FALIKVEAINFEAPGETRDKVFFDNLTPLYPEEQLRLETASDSLSARVMDLVTPLGKG
                     QRALIVAPPRTGKTMLLQTIANSISQNHPEVTLIVLLIDERPEEVTDMQRSVNGEVIS
                     STFDEPPTRHVQVADMVIEKAKRLVEHKRDVVILLDSLTRLARAHNAVVPPSGKILSG
                     GIDANALQRPKRFFGAARNIEEGGSLTIIATALIDTGSRMDDVIFEEFKGTGNSEIHL
                     DRRLSDKRLFPAIDLQRSGTRKEELLISKEDLNRIWVMRRVLNPLSPVEQMEVVLERL
                     GKTKTNADFLASMQS"
     gene            complement(4432..5046)
                     /gene="yihA"
                     /locus_tag="AC159P_10435"
     CDS             complement(4432..5046)
                     /gene="yihA"
                     /locus_tag="AC159P_10435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013841055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HIR3695908.1"
                     /translation="MKISSAEFVLSAFAESQWPRGGLPEVAFLGRSNVGKSSLINSLL
                     GVRGLARTSNTPGRTQALNFFLINRRFHFVDLPGYGYARVPRDVRESWGQLVTGYLAK
                     REPLVLSIQIVDSRHEPTSLDLQLREWLLAEGKPFLTVATKSDKLSQNELSRNLARAR
                     QVLRAAGDGSGDEMVAYSAVTRRGRENVWRAIEEALAASPQPPS"
     gene            complement(5057..5344)
                     /locus_tag="AC159P_10440"
     CDS             complement(5057..5344)
                     /locus_tag="AC159P_10440"
                     /inference="COORDINATES: protein motif:HMM:NF018404.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClpX C4-type zinc finger protein"
                     /protein_id="HIR3695909.1"
                     /translation="MTAETKDLLRCSFCGKSQREVEQLVNGPNVRICNNCVDICIEII
                     ADARAAAGAADERAGESARPRTPEVVHVGTFTCPRCRVEFALHTEAPEDHS"
     gene            complement(5455..7896)
                     /gene="lon"
                     /locus_tag="AC159P_10445"
     CDS             complement(5455..7896)
                     /gene="lon"
                     /locus_tag="AC159P_10445"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014785221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HIR3695910.1"
                     /translation="MEEYSDRTPHDIARLPMVPIRDVVIFPFTKVAFKIGRPGSVRAL
                     EDALSTDRTIFLAPQHDASIDEPVPEQIYTVGTIARILQAQRQENGQTKVVVEGRERA
                     TMIRVENNNGAFTATIRRAPSAKEDGPRIEALMQRIHQLVEQHVRLAPETQTDALQTA
                     LRNQDPSHLADALASQLKISVEDKQGLLEIFSAHGRLQRLVELMEVEVDKRQLDRTIQ
                     ARVKRQMEKAQKEYYLNEQIKAIHKELGRKDEKAEVEDLKKRIEEAGMTEEAKEKALQ
                     ELHRLEAMPPMSAEGTVSRTYIEWLLSVPWKQKSKEIKDITKAEEVLNEDHYGLEKIK
                     DRILEHLAVRQLVKNPKGSILCFVGPPGVGKTSLGKSIARATGRKFVRLSLGGVRDEA
                     EVRGHRRTYIGALPGQIIQMMKKAGTVNPCILLDEVDKLGADFRGDPSAALLEVLDPE
                     QNNTFQDHYLDVEYDLSKVFFIATANVLHTIPPALQDRLEILRLSGYTEREKLEIAKR
                     HLVRKQAEGSGLKPEQVEFTDEGILEIIRHYTREAGVRNLEREIGSCCRKVARKLVAD
                     AAAEEFKVVLAPELVREMLGPMKFRQQPVAASSEVGLAVGLAWTEVGGEVLQIEATLT
                     NGRGGVQLTGKLGEVMQESAQAALTCIKARAERLGMNLEFIRKRDLHIHIPEGAIPKD
                     GPSAGVTLATAMASALTRVPVRKDLAMTGEITLRGRVLPVGGIKEKLLAAHRLGQTTV
                     LIPKDNEKDLQEVPEEVLADMTIQLVETIDEVLSLALEDACPTDAGATDEEASHPPVW
                     TTDKPSAGTPTGMAD"
BASE COUNT         1274 a         2585 c         2720 g         1551 t
ORIGIN      
        1 atgaagtaga agctgcattg gccgcgcacc tcttcgatgt cgcgctgctc gatggggaag
       61 aggcggcact ggttggggcg gtcttcgtag atgccgcagg tggtcgagcc gtcctcgtgc
      121 ttcttgagga cagggcactt gaacagcagc tcgcagcagg cgccgcagcg gttgcactcg
      181 ccgcgccgga gcgagagcgc ctcgatcacc tcctccttgc ggaagcggat gttgacgaag
      241 cggcggaact tgccctgcgc ctgcttggcg cgcagcttcg cgtcctcgac ggcgcgggcg
      301 cgtttctgcg ccagccgctc ctcgaacgag cgccggctgt tgccgctgct gttgcgcgcg
      361 tggcccgcgc ggcttttgga ggtctgtttc atcgcaagga accgccgacg tgaccgtcgc
      421 cgctcggtcg ggcggcgcgg gccgcgggaa gagtgggtac agcgacgaac cgcctcgcac
      481 acacgagggc ggcgcgagcg cgcgccccga cgcgccaacg cccgcgccgc caagctgtca
      541 acgagtccgt caggaggcgc tcgcggctgt cccgacgtac ttgtcgatca tgcgcgagat
      601 ggcctccttc gaggtggcgc cgacgacccg ctcctcctcc ttgccgttct tgaagaggat
      661 cagcgtcggg atgcccttga tgccgtagcg ctgcgagatg gcggggttgt catcgacgtt
      721 gagcttgacg accgtcgcgc tcccggcgta cttctcggcc acggcctcga ccgtcggcgc
      781 gagcatccgg cagggcgcgc accactgcgc ccagaagtcc acgaggacag ggcgatccga
      841 cttcaaaacg tccgcctcga aactggtgtc gctgacatct ttcacgtgtt cactcatgtt
      901 cgaactctcc ttgatcaaaa ctttccgcgg gcggggttaa gatttcgact ctccgaaaaa
      961 cccgcccgat gtttggatgt aaatcggttg cccgtcgttc cggcagccct tcgtgtcact
     1021 gtcacgccgc ggtcacatgc gcgccaccgc gcggtaaacc tcgatgtact tgcgcgccgc
     1081 cgccgcccac gagttgtctt cgcgcatgcc gttcaactgg atcgtgcgcc agacctctgg
     1141 ctggcggtag cagtagagcg cctcgtagat tttttcgagc atcgcgtgcg ccgagtaggg
     1201 gccgaacttg aagccgctgc ccgcggcgcg cgcgcggtcg aagttctcca ccgtgtcgtc
     1261 gaggccgccc gtcgcgcgca ccaccggcac cgtgccgtag cgcatgctgt acatctggtt
     1321 cagcccgcac ggctcgtact gcgagggcat caggaagatg tcggcgcccg cctcgatccg
     1381 gtgcgcgagc ggctcgctga agcccttgta aatcccgacg cggctgggcg cgtggtcgtg
     1441 cagcgcctgc aaaaaatcct cgtactccga ggcgcccgag ccgagcgcga cgaagaagac
     1501 gcccgcttcg agaatgcgcc cggcggcctg cttgacgagg tcgaagccct tctgcggcgt
     1561 cagccgcgag acgcacgcca cgacggggcg ttccggctcc tccggcagcc cgaagctgcg
     1621 cagcaggtcg agcttgcacg cgcgcttgcc cgtcaggtct tccgccgaat agttcgccgc
     1681 cagatgcggg tcggtcgcgg ggttccacac gtcgtaatcg acgccgttcg tgatgccgat
     1741 gaggcggtcg cggcgcaagc gcaaaagcca gtcgaggccg tgcccgtgct cgggcgtctg
     1801 tatctcgaac gagtagcggc ggctgacggt cgagagcgcg tccgaggtca tcagccccgc
     1861 tttgagcacc gacgccacgc ggtctttcag gaagacgttc tgctcgtcgc cgtccttgaa
     1921 gccgaggcgc cacaagtcgt ccgggtcgaa caggccttga taggcgaggt tgtggatcga
     1981 gaagagcgtg cgcgtctgcg agaggaagcc gccgtagggg cggcccgcct tgagccacac
     2041 ggcggcgaag ccgcagggcc agtcgttgcc gtgtaccacg tcgggcgccg ggccgaggcg
     2101 gcccaggagg gcgagcgcgg cgcggcagaa gaaggcgaag cgctcgtggt cgtcgcggta
     2161 gccgtacacg tccgggcgca tgaagtactc gggcgcgttg atgaggaacg aaggcgcgcc
     2221 cgtcgcctcg ctgtaccaca cggaggcgcg gcgcaccccg cccagccact cgacttcgag
     2281 gttgtcaaag acctgctcct gaaggtgctc gcgcttcgtc tgcccgtgca aggggtggat
     2341 gagcaccaca tccgccccct cgttctgtag cgccttcggc agcgcgcccg cgacatcgcc
     2401 gaggccgccg gtcttggcga agggcaccgc ctcggatgaa agaatcgcga cgcgcaagct
     2461 cttttccgac accgcgcccc cacaggagaa acgaaaaaca caggactgga ttgctaagac
     2521 taggaagccc cactatacag gaaaagcccc gattcgtgaa ccggtaaaaa gccgcgaaac
     2581 gtgaaccgta aaccgtgata agtcagaagc ttttcctact tatcacggtt tacgattcac
     2641 gtttcgcggc ctttagctct gcatcgaggc gaggaagtcg gcgttggtct tggtcttgcc
     2701 gagacgttcg aggacgacct ccatctgctc gacgggcgag aggggattga ggacgcggcg
     2761 catgacccag atgcggttga ggtcttcctt cgagatgagc agctcctctt tgcgcgtccc
     2821 cgaccgctgg aggtcgatgg ccgggaagag gcgcttgtcg gagaggcgcc ggtcgaggtg
     2881 aatctcggag ttgccggtgc ccttgaactc ttcgaagatc acgtcgtcca tgcgcgagcc
     2941 ggtgtcgatg agcgccgtgg cgatgatcgt caaagagccg ccctcctcga tgttgcgcgc
     3001 cgcgccgaag aagcgcttgg ggcgctggag ggcgttggcg tcgataccgc cggagaggat
     3061 tttgcccgac ggcgggacga ccgcgttgtg cgcgcgcgcc agacgggtca agctgtcgag
     3121 caggatgacc acgtcgcgct tgtgctcgac gaggcgcttg gccttctcga tgaccatgtc
     3181 ggcgacctgt acgtggcgcg tcggcggctc gtcgaaggtc gaggagatga cctcgccgtt
     3241 gaccgagcgc tgcatgtcgg tgacttcctc agggcgctcg tcgatgagga gcacgatgag
     3301 cgtcacctcg gggtggttct gcgagatcga gttggcgatg gtttgcagca gcatcgtctt
     3361 gccggtgcgc ggcggcgcga cgatgagcgc gcgctgcccc ttgccgagcg gcgtcaccaa
     3421 atccatcacg cgcgccgaga ggctgtcgga cgcggtctcc agccggagct gctcttccgg
     3481 gtagagcggc gtcaggttgt cgaagaagac cttgtcgcgc gtctcgccgg gcgcctcgaa
     3541 gttgatggcc tcgaccttga tgagcgcgaa gtagcgctcg ccctctttgg gcgggcgaat
     3601 ctgcccggac acggtgtcgc cggtgcgcag gtcgaacttg cggatctgcg aggggctgac
     3661 gtagatgtcg tcggggccgg gcaggtagtt gtactcgggt gcgcgcagga agccgaaccc
     3721 gtcgggcagg gtttccagca cgccctcgga gaagatcagc ccgctcttct cggtctgcgc
     3781 ggcgaggact ttgaagatca gctcctgctt gcgcatcccg gtggcgccgg gcacgtccag
     3841 gtccttggcg atgccggtca gcttggagat ggacatctcc ttgagattcg ccaggttgaa
     3901 cggctcgccg tcgaagggcg agacgccggc ggcagcctcg gcagcctcaa gctccgcgtc
     3961 agcgtccata tccacatcca catccacgtc gggcggcggc gcctcgacgg tcgcggtctt
     4021 cgcgttgccg tcgctgagcg aggcgtccgc ggcgcggtct ttattaatgc gtgttcttgt
     4081 tcgtgacatt gtctttcttc tctggcggcg cctccacccg aaggtgagcg cccgttgttg
     4141 actgcggagc gcgcgccgcg tctctacttg cgtgcaacgc gggcggcgcc ggtctgcctt
     4201 tcctttagga tttgtatggt tgccgattgt acgtcaggaa agatgcctct tggtgcgccc
     4261 gccgtcggcg cgcttcgtgg agctgcggcc cctctcgtgt ggcgcgggcc tggtctcaag
     4321 gcggcccgcc tcttctcccg aactcaaaaa tttaccggcg gattattggg ctggaggtta
     4381 gatgccccaa acgcccaccc ggtagccctg cctttcgcgc ttcgtctttt ttcaagaggg
     4441 gggttgtggt gaggcggcca gtgcctcttc gatggcgcgc cagacgttct ctcgaccgcg
     4501 ccgcgtcacg gcggaatagg cgaccatttc gtcgcccgag ccgtcccccg ccgcgcgcaa
     4561 aacttggcgg gcacgtgcga ggttcctgct cagctcgttt tgcgagagct tgtcggactt
     4621 agtcgctacc gtcagaaacg gcttcccttc ggcgaggagc cattcgcgga gctgtaaatc
     4681 caatgacgtg ggttcgtggc gcgaatctac gatttgaatt gatagcacaa gcggctcccg
     4741 cttggcaagg tagccggtga cgagctgccc ccacgactcg cgcacgtcgc gcggcacgcg
     4801 cgcgtaaccg taccccggca gatcgacgaa atggaagcgc cggttgatca aaaaaaagtt
     4861 gagcgcctgc gtgcggcccg gcgtgttcga cgtgcgcgcc agcccccgca cgcccagcag
     4921 cgagtttatc aaactggact tgccgacgtt cgaccgcccg aggaaggcca cctccggcag
     4981 ccccccgcgc ggccactggc tctcggcgaa cgcactcagg acgaactcgg cgctgctgat
     5041 tttcatgcct gacgggttac gaatgatctt cgggcgcttc cgtgtgcagc gcgaactcga
     5101 cgcggcacct ggggcacgtg aacgtcccga cgtgcacgac ctccggcgtc cgggggcgtg
     5161 cgctctcgcc cgctcgttcg tccgcggcgc ccgccgcggc ccgcgcgtcc gcgatgattt
     5221 cgatgcagat gtccacgcag ttgttacaaa tcctgacgtt cgggccgttg acgagctgct
     5281 cgacctcgcg ttgggatttc ccacagaacg aacagcggag gaggtctttg gtctcggcgg
     5341 tcatggctct tagaggcgcg ggctgacgag ggcgggggaa agggacgggt tgaaggcgac
     5401 ggcgccgacg gcgacaggca agtttccgcc ggtcgccgtc ggccccttcg aagctcagtc
     5461 ggccatgccc gtcggcgtgc cggcggaggg cttgtcggtc gtccagacag gcgggtggct
     5521 cgcctcctcg tcggtcgcgc ccgcgtccgt ggggcaggcg tcttcgagcg cgagcgagag
     5581 cacctcgtca atcgtctcga cgagctggat ggtcatgtcg gccagaactt cttcgggcac
     5641 ttcctgcaag tccttctcgt tgtccttcgg gatgagcacg gtcgtctgcc cgagcctgtg
     5701 cgcggccagc agcttctcct tgatgccgcc cacgggaagc acgcgaccgc gcagcgtgat
     5761 ctcgcccgtc atcgccaggt ctttacggac ggggacgcgc gtcagcgccg aggccatcgc
     5821 ggtcgccagc gtgacgcccg ccgaggggcc gtccttcggg atggcgccct cggggatgtg
     5881 gatgtgcagg tcgcgcttgc ggatgaattc gaggttcatg ccgagccgtt cggcgcgggc
     5941 cttgatgcag gtcaaagcgg cctgcgccga ctcctgcatc acctcgccga gcttgccggt
     6001 gagctggacg ccgccgcgac cgttcgtcaa ggtcgcctcg atttgcaaga cctcgccgcc
     6061 cacctccgtc cacgcgagtc cgaccgcaag gccgacctcg ctcgaagcgg ccacgggctg
     6121 ctggcggaac ttcatcggcc ccagcatctc gcggacgagt tcgggcgcga gcacgacctt
     6181 gaactcttcg gcggcggcgt cggcgacgag cttgcgcgcc accttgcggc agcacgagcc
     6241 gatctcgcgc tccaggttgc gcacgcccgc ctcgcgcgtg tagtggcgga tgatttcgag
     6301 aattccctcg tcggtgaact cgacctgctc gggcttcaga ccgctccctt cggcctgctt
     6361 gcggacgagg tggcgcttgg cgatctccag cttctcgcgc tcggtgtagc ccgagagccg
     6421 caggatttcc aggcggtctt gcagcgcggg cgggatcgtg tggagcacgt tcgccgtggc
     6481 gatgaagaag accttcgaga ggtcgtactc gacatcgagg tagtgatcct gaaacgtgtt
     6541 gttctgctcg gggtctaaga cttcgaggag cgccgccgac gggtcgccgc gaaaatccgc
     6601 gccgagcttg tccacttcgt cgagcaggat gcacgggttg accgtgccgg ccttcttcat
     6661 catctggatg atctggccgg gcagcgcgcc gatgtaggtg cggcggtggc cgcgaacctc
     6721 cgcctcgtcg cgcacgccgc ccagagagag gcgcacgaac tttctgccgg tggcgcgggc
     6781 gatggacttg cccagagagg tcttgccgac gcccggcggg ccgacgaagc agaggatcga
     6841 gcctttcggg ttcttgacga gctggcgcac cgcgaggtgc tcaagaatcc ggtctttgat
     6901 cttctccagg ccgtagtggt cttcgttcaa gacctcctcg gccttcgtga tgtccttgat
     6961 ctctttcgac ttctgcttcc atggcacgct cagcagccac tcgatatagg tgcgcgagac
     7021 ggtgccctcg gcgctcatcg gcggcatggc ttcgaggcgg tgcaattcct ggagggcttt
     7081 ctccttcgcc tcctcggtca tgcctgcctc ttcgatgcgc ttcttcaggt cttcgacctc
     7141 ggccttctcg tccttgcggc ccagctcctt gtggatggcc ttgatctgct cgttgaggta
     7201 gtactccttc tgcgccttct ccatctgccg cttgacgcgc gcctgaatcg tgcggtcgag
     7261 ctggcgctta tcgacttcga cctccatcag ctcgacgagc ctttgcaggc gaccgtgcgc
     7321 ggagaagatt tccaagaggc cctgcttgtc ctcgacggag attttgagct gcgaggcgag
     7381 cgcgtcggcg aggtgcgaag ggtcttgatt gcggagcgcg gtctggaggg cgtcggtctg
     7441 cgtctcgggc gcgaggcgga cgtgctgctc gacgagctgg tgaatgcgct gcatgagcgc
     7501 ctcgatgcgc gggccgtcct ctttggccga gggcgcgcgc ctgatggtgg cggtgaaggc
     7561 gccgttgttg ttctcgacgc ggatcatggt cgcgcgctcg cgcccctcga ccacgacctt
     7621 cgtctgcccg ttctcctgcc gctgcgcttg caggatgcgg gcgatggtgc cgacggtgta
     7681 aatctgctcg ggcaccggct cgtcgatcga ggcgtcgtgc tgcggggcga ggaagatcgt
     7741 gcggtcggtc gagagcgcgt cttcgagcgc gcgcaccgag ccgggccgac cgatcttgaa
     7801 ggcgaccttc gtgaaaggga agatgacgac atcacgaatc ggcaccatcg gcaggcgcgc
     7861 gatgtcgtgc ggggtgcgat cagaatattc ttccataaat cgaatctact tgccttggga
     7921 cagccggatt accgcgcatg gtgcaaaggg cgagcctctc ggaagtcgct cgccccggca
     7981 cttactgaaa gcaattctaa accatttcga gaccgcgcgc cacagggcaa attaaaagta
     8041 aaaaggtaaa aggtaaaagg caaaagctgg gaagcctcca cacttttgcc ttttaccttt
     8101 tgctttaact ggcttacgcg gcttcgtcga
//
LOCUS       DBNGOH010000536         2414 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_628381, whole genome shotgun sequence.
ACCESSION   DBNGOH010000536 DBNGOH010000000
VERSION     DBNGOH010000536.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2414)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2414)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2414
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_628381"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1654
                     /locus_tag="AC159P_10450"
     CDS             <1..1654
                     /locus_tag="AC159P_10450"
                     /inference="COORDINATES: protein motif:HMM:NF018884.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HIR3695911.1"
                     /translation="PFASRVHAEVRREGDQYFLQDLGSANGTYYDGARAEGPLPLTPG
                     GRIQIGETEIVFRDSAAAGELTAATVEEEGSAPVPEATIAFDSAHRTTSGLLEAIEGA
                     RTQTSGRHSAPVPHIMPHHAPPSGGAAAAAADQSDLLALISKVGVTLLASATLDETLR
                     QVARLVFEAVPAERCLIMLRDAGEKELKVRAAEMRSHKPDVGEIRVSRTIVEEVVGQG
                     RSVLTSDAQHDPRFMSSTVTFQGIRSVLAVPLGVGAQIFGMIYADSPLTEARFTEDHL
                     KVLTTLGSVAAIRVEKAELLEEHLEQERINRELQLAREIQQRFQPTSPPAVKGYEFQG
                     ISFPSYEIGGDYYDFIKRRDGRYVVALGDVSGKGTSAALLMSSLHAAVHAQVASNQSL
                     TDTIAAVNRYLADNTPPNRFVTLFYGELDPLTGSLSFINAGHNPPLIFHDAGTVEQLG
                     ASGLPLGIMPDFAYREGRTQLQPGDVLVAYSDGVTETVNPKGEEFGPARLQEIISRTL
                     HSSAAGIRDKIEAALSSFAQGTPAVDDITLVIVKRAREEKKS"
     gene            1926..2345
                     /locus_tag="AC159P_10455"
     CDS             1926..2345
                     /locus_tag="AC159P_10455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HIR3695912.1"
                     /translation="MPLVPRFEFFRRHREYGALFVRLVVGLILVHQSQDNVFSHARML
                     EFRDFLAARQVPWPLVAAHVSVYAQFICGILYLLGLFVRPAALLMIVNFIAALVIAHR
                     NQPIEGAFLALVMLFCSLFLLFNGAGKPSIDDRMERR"
BASE COUNT          407 a          918 c          717 g          372 t
ORIGIN      
        1 cccgttcgca tcgcgcgtcc acgccgaggt gcggcgcgag ggcgaccagt atttcctgca
       61 agacctcggc tcggccaacg gcacctacta cgacggcgcg cgcgcggagg ggccgctgcc
      121 cctgacgccc ggcgggcgca tccagatcgg cgagaccgag atcgtcttcc gcgacagcgc
      181 cgccgcgggc gagctgaccg ccgccaccgt cgaagaggag ggcagtgcgc ccgtccccga
      241 agcgaccatc gcgttcgact ccgcgcaccg caccacctcg ggcctgctcg aagccatcga
      301 gggcgcgcgc acgcagacct cgggccgcca ctcggcgccc gtcccgcaca tcatgccgca
      361 ccacgccccg ccctccggcg gcgccgcggc ggcggcggca gaccagagcg acctgctcgc
      421 gctcatcagc aaagtgggcg tcacgctcct cgcctcggcc acgctcgacg agaccttgcg
      481 gcaggtggcg cggctcgtct tcgaggccgt gccggcggag cgctgcctca tcatgctgcg
      541 cgacgcgggc gagaaggagc tgaaggtgcg cgccgcggag atgcggagcc acaagcccga
      601 cgtgggcgag attcgcgtca gccgcaccat cgtcgaagaa gtggtcgggc aggggcgctc
      661 ggtcttgacc tcggacgcgc agcacgaccc gcgcttcatg tcgagcaccg tcaccttcca
      721 gggcatccgc tcggtgctgg ccgtgccgct cggcgtcggc gcgcaaatct tcggcatgat
      781 ctacgccgac tcgccgctca ccgaggcgcg cttcaccgaa gaccacctca aggtgctgac
      841 gacgctcggc tcggtcgccg ccatccgcgt cgagaaggcg gaactgctcg aagagcacct
      901 cgaacaggag cgcatcaacc gcgagctgca actggcgcgc gagatacagc agcgcttcca
      961 gccgacctcg ccgcccgccg tcaagggcta cgagttccag ggcatcagct tcccgtccta
     1021 cgagatcggc ggtgactact acgacttcat caagcggcgc gacgggcgct acgtcgtcgc
     1081 gctcggcgat gtgtcgggca agggcacctc cgccgcgctc ctcatgtctt ccttgcacgc
     1141 ggcggtgcac gcgcaggtcg cctcgaacca gtcgctcacg gacaccatcg cggcggtcaa
     1201 ccgctacctc gccgacaaca cgccgcccaa ccgcttcgtc acgctcttct acggcgaact
     1261 cgacccgctg accggctcgc tctccttcat caacgccggc cacaacccgc cgctcatctt
     1321 ccacgacgcg ggcaccgtcg aacagctcgg cgcgagcggc ctgccgctcg gcatcatgcc
     1381 cgacttcgcc taccgcgagg ggcgcacgca gctccagccc ggcgacgtgc tcgtcgccta
     1441 ctccgacggc gtcaccgaga ccgtcaaccc gaagggcgaa gagttcggcc ccgcgcgcct
     1501 acaggagatc atcagccgca ccctccactc ctccgccgcg ggcatccgcg acaagatcga
     1561 ggccgccctc tcctccttcg cgcagggcac gcccgccgtc gatgacatca ccctcgtcat
     1621 cgtcaagcgc gcgcgcgagg agaagaagag ctgagccgat tgcggaatgc ggatttcgga
     1681 ttgcggattt gcagaagcag gaagatcgac ggaagcatca ctctcatcat cgatgaaggg
     1741 ggcacgctct tcattgaaga cagcggctcc ttcatcaacc acgaatcgca atccgcattc
     1801 cgcaatcgaa taatccgcat tccgcaatcg gttccttccg tctcgtcaca aaccgcttcc
     1861 gcgcggcgtc cgcgtcgtag tacaacaccc gcgcagtatc atcccgcccc ccggagccat
     1921 cgccgatgcc gctcgtcccc cggttcgaat ttttcaggag gcaccgcgag tacggcgcgc
     1981 tcttcgtccg tctcgtcgtc ggactcatcc tcgtccacca gagccaggac aacgtcttca
     2041 gccacgcgcg catgctcgaa ttcagagact tcctcgccgc gcgccaggtg ccgtggccgc
     2101 tcgtcgcggc gcacgtctcg gtctacgcgc agtttatttg cggcatcctc tacctcctcg
     2161 gcctcttcgt ccgccccgcc gccttgctga tgatcgtcaa cttcatcgcc gccctcgtca
     2221 tcgcgcaccg caaccaaccc atcgaaggcg ccttcctcgc gctcgtcatg ctcttctgct
     2281 cgctcttcct cctcttcaac ggcgcgggca agccgtccat cgacgaccgg atggagagac
     2341 gctgaaagat gacggggcgg caaggggaag gggcgacggg gagaagccgt acaccgaacg
     2401 ccgcttctct cccc
//
LOCUS       DBNGOH010000537         1686 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4660506, whole genome shotgun sequence.
ACCESSION   DBNGOH010000537 DBNGOH010000000
VERSION     DBNGOH010000537.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1686)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1686)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1686
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4660506"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1384)
                     /locus_tag="AC159P_10460"
     CDS             complement(<1..1384)
                     /locus_tag="AC159P_10460"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014425.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HIR3695913.1"
                     /translation="MTSPPRTAEPAHGASRAAPHASLLSQWRWWLPVALLSLALSFIF
                     QDPFAGDWDSLDYTVLAVNGWEPSSMLLGRMLYIFTNRVAYLITHALFGLPAEKAYLL
                     FKYMVIAQVPLATAALWALARELSGSARAATVAALMLALSPYYVVYGGQAMTEIPSIL
                     LLCAALTIHLRALRQRELGKMLLGAALLGLGVNLREGVGLFGLWLVLAPFACGWRLRA
                     RDLMITAAACAVFFVLALGPFAVWYAWDVNGYQGKWWGWVESTKMESALHPVALENFR
                     PLFRWFLIASPLSLVLLPFAMFREWRARGFSPLFALGAVGLFANLSLISHYSTVINGR
                     YLLTGLPALVPLVADFLTRVLDPRKLFGFFDGRRTTIDVGQPTTTGDEPEATTDDDEF
                     EATTDDGRRRGFVPALVVVLVVGLWMGWRIYPEAWPTIQHHATWKDYRARLEEIPRDN
                     SVVIAGGQTVA"
     gene            complement(1359..>1686)
                     /locus_tag="AC159P_10465"
     CDS             complement(1359..>1686)
                     /locus_tag="AC159P_10465"
                     /inference="COORDINATES: protein motif:HMM:NF024970.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR3695914.1"
                     /translation="NAVKHGNEGDPKKLVRITADLSTKEARFTIEDEGAGFNVKEIPD
                     PLDPSNLFKTSGRGVLLIYNIMDVVEYNERGNRLTMVKYAESSLDKELIDTLAPTGND
                     IPAPNG"
BASE COUNT          316 a          564 c          556 g          250 t
ORIGIN      
        1 cggcgaccgt ctgcccgccc gcgatgacca ccgagttgtc gcgcggtatc tcttcgagcc
       61 gcgcgcggta atccttccac gtcgcgtgat gctgaatcgt cggccacgcc tccgggtaga
      121 tgcgccagcc catccacagg ccgacgacca agaccacgac gagcgcggga acgaagccgc
      181 gtcgccttcc gtcatcggtc gtcgcttcga actcgtcgtc gtcggtcgtc gcttccggtt
      241 cgtcgcctgt cgtcgtcggc tgtccgacat caatcgtcgt ccgccgcccg tcgaaaaatc
      301 cgaagagctt ccgcgggtcg aggacgcgcg tcaggaagtc cgcgacgagg gggacgagcg
      361 cgggcaggcc cgtgagcagg tagcgcccgt tgatgaccgt gctgtagtgg gagatgagcg
      421 agaggttggc gaacaggccg accgcgccca acgcgaagag cggcgagaag ccgcgcgcgc
      481 gccactccct gaacatggcg aagggcagca gcacgagcga gagcggcgac gcgatgagga
      541 accagcggaa gagcgggcgg aagttttcca aagcgaccgg gtgcagcgcc gactccatct
      601 tcgtcgattc gacccagccc caccacttgc cttgatagcc gttcacgtcc catgcgtacc
      661 acaccgcgaa cggcccgagc gccagcacga agaagaccgc gcacgccgcc gccgtaatca
      721 tcaaatcacg cgcccgcaac ctccaaccgc acgcgaacgg cgcgagcacc agccacaacc
      781 cgaacagccc gacgccctcg cgcaggttca cgcccaggcc cagcagcgcc gccccgagca
      841 gcatctttcc caactccctc tgcctgagcg cgcgcaggtg aatcgtgagc gccgcgcaca
      901 agagcaggat cgacggaatc tccgtcatcg cctgcccgcc gtagacgacg tagtagggcg
      961 acagagcgag catcagcgcc gccaccgtcg ccgcccgcgc cgaacccgac aactcgcgcg
     1021 cgagcgccca cagcgccgcg gtcgccagcg gcacctgcgc gatgaccatg tacttgaaca
     1081 ggaggtacgc cttctctgcc ggcagcccga agagcgcgtg cgtgatgaga taagcgacgc
     1141 ggttcgtgaa gatgtagagc atccgcccga ggagcatcga ggacggctcc cagccgttga
     1201 cggcgagcac cgtgtagtcg agcgagtccc agtcgcccgc gaacgggtct tgaaagatga
     1261 aggataacgc gagcgacagg agcgcgacgg gcagccacca gcgccattgc gaaagaaggg
     1321 atgcgtgcgg ggcggcgcgc gaggcgccgt gcgcgggttc agccgttcgg ggcggggatg
     1381 tcattgccgg tgggcgcgag cgtgtcgatc agttccttgt cgagagagct ttccgcgtac
     1441 ttcaccatcg tcaggcggtt gccgcgctca ttgtactcga ccacgtccat gatgttgtag
     1501 atgagcagca cgccgcggcc cgaagtcttg aagaggttcg aggggtcgag cgggtcggga
     1561 atctccttga cgttgaagcc cgccccctcg tcctcgatgg tgaagcgcgc ctctttggtc
     1621 gaaagatcgg cggtgatgcg gacgagcttc ttggggtcgc cctcgttgcc gtgcttgacg
     1681 gcgttg
//
LOCUS       DBNGOH010000538         6739 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1180485, whole genome shotgun sequence.
ACCESSION   DBNGOH010000538 DBNGOH010000000
VERSION     DBNGOH010000538.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6739)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6739)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6739
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1180485"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..494
                     /locus_tag="AC159P_10470"
     CDS             <1..494
                     /locus_tag="AC159P_10470"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016846516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3695915.1"
                     /translation="MAEAYPASDFVGYDYHDGSIEMARKRAEEAGLSDRVKFEVARAK
                     DYPGNGYDFVAFFDCLHDMGDPRGAAAHVRETLAPDGTWMIVEPFAGDRAEDNLNPVG
                     RIYYSASTLLCTPASLSQEVGLALGAQAGEARLREVVTGGGFSRFRRATETPFNIVYE
                     AKP"
     gene            complement(610..2028)
                     /locus_tag="AC159P_10475"
     CDS             complement(610..2028)
                     /locus_tag="AC159P_10475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007356366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HIR3695916.1"
                     /translation="MKVKMILPALTEAKSPFFRPVKYSLFPPLGLATLAGYLDDGTQV
                     SLQDEHVETLDTEDAPELVVIQVYITSARRSYELADHYRRCGAYVVLGGLHVTSLPDE
                     AAAHADTIFLGPGEDTWPAFLSDYRAGRPQKVYRSTVRTLDRLPRIRRDLIKRHLYLV
                     PNSIVVSRGCPHSCDFCYKEAFFEGGRGFYTQKVDDALAEIERLPGRHLYFLDDHLFG
                     DTRFAEALFDGMRGMNRLWQAAGTVQSVLRPRLLEKAVACGLRSLFVGFETLSADNLR
                     EQRKPHNLGRDYGAAIRRLHDLGVMVNGSFVFGMDGDDEGVFERTVEWAVGQGIETAT
                     FHILTPYPGTALYERMLKAGRMLHDDWDLYDTRHAVYRPARMTPEKLEQGYRRAYRDF
                     YRWRSILDGAATKPTLLEAARHFAYAAGWKKFEPLWDMVIRAKRVSHTLPILEAILKG
                     FSRHPSDEQFGAAQELAGSVYR"
     gene            2254..3447
                     /locus_tag="AC159P_10480"
     CDS             2254..3447
                     /locus_tag="AC159P_10480"
                     /inference="COORDINATES: protein motif:HMM:NF025030.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HIR3695917.1"
                     /translation="MISNIRVSLRGLLLACCASAACSPLLHTAFAQEGADQQTQSLVR
                     RLQEGDTQSRRAAAEEIKGRGAAAKAAVPALALALKDPDDELRKTAAEALGRMGPEAR
                     SAVPALTEALRDGNGSVRSNAAFALGQVGAEAESAVPALTGLLKDPSPWARAGAARAL
                     GRIGGQAKASVPAVVELLKDRNGGVRGDAAEALGRFGGDAGAAVPAVIVLLKDESASV
                     RKAAAEALGRIGPGARGAVPALAESMKDREFVVRMNAAQALGRIGMEPAEAVPVWMGS
                     LGDPSEHVRRYAAEALAEIGPGARAAVPALVGALKDQDRFVRLSAAKGLGNIAGEARA
                     AVPDLVGLLRDEEPLVRANAATALGKLGGEARTALPTLNELLKDSATVVRLAAAEAIN
                     KIASQ"
     gene            3662..4357
                     /locus_tag="AC159P_10485"
     CDS             3662..4357
                     /locus_tag="AC159P_10485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009718449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695918.1"
                     /translation="MSPEWQEETADSASLREQLRHVYWIGGGSGAGKSTVARRIAARH
                     SLRVYATDDVMADHASRSTSEDSPLLHRFMAMGMDERWVNRPPDTMLDTFHWFRGEGF
                     DLIVEDLLSLPRETGVIAEGFRLLPRLVAPLLSAPARAVWLLPTPTIRQAVVESRGGA
                     ASGFLARTTDPERALRNLLERDRMFTDKLREEAARLGLSVIDVDGAMTEDDLAGRVTE
                     VFGLYSGASFQHE"
     gene            4604..6682
                     /locus_tag="AC159P_10490"
     CDS             4604..6682
                     /locus_tag="AC159P_10490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008104648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HIR3695919.1"
                     /translation="MTFSAHPLGGIVNRLSVSSGGEPAMLSKIIRSVFVGLILSAFIA
                     PAGHAQKVTLRIQSDVSGRPISPDLFGIFFEDLNYAADGGLYAELVQNRSFEYQATEQ
                     PTWNSLSFWELTKRGGGEGKWAVEYARPLHTNNPHYLALEVTKAADGVGLANPGFDGI
                     ALKAGETYDLSLFARQLFMNRRWPPDNSIEGKPMPLAVRLESKDGEVLSEAPLQVTGR
                     EWTRLTATLTPKRDEDAARLVLLAKANGGIALDEVSLFPRKTFRDRPNGLRADLAQTI
                     ADLRPKFIRFPGGCLVHGNGTNNFYRWKDTIGPTEQRRGQANLWGYHQSVGLGYFEYF
                     QFAEDIGAKPLPVVAAGVSCQNSDHTGEVGQQCLAIADMPAYIRDILDLIEWANGPAT
                     SRWGAKRAAAGHPKPFNFEYLGVGNEDVISEEFRVRFKMIRDAVRAKHPEIRVVGTVG
                     PFPEGPDYEAGWKFANEQRLEMVDEHGYKPPEWYWQNLSRFDAYDRTKSKVYLGEFAA
                     HETNRANTLRSALAEAAYMTSLERNGDVVRLASYAPLLARQGHTQWRPDLIYFDGVKV
                     LRTANYYVQQLFSLNQGDAYLTNALTGVPRPDQFAVSSVRDGLTGDIIIKLVNGSPAP
                     LPLLVELTGAKKISRRALKTVLAGEAAAVNGFDAPSRVAPVTSGIQVDRQFEYEAPAH
                     SLTVIRIETR"
BASE COUNT         1210 a         2149 c         2251 g         1129 t
ORIGIN      
        1 tgatggccga ggcctacccg gcctcggact tcgtcggcta cgactatcac gacggctcca
       61 tcgagatggc gcgcaagagg gcggaggagg ccggcctgtc cgaccgcgtg aagttcgagg
      121 tcgcgcgcgc gaaagactac cccggaaacg gctacgactt cgtggccttc ttcgactgcc
      181 tgcacgacat gggcgacccg cggggcgcgg cggcgcacgt gcgggagacg ctcgcgcccg
      241 acggcacgtg gatgatcgtc gagccgttcg ccggcgaccg agccgaggat aacctgaacc
      301 ccgtggggcg catctactac tcggcctcga cgctgctgtg cacgcctgcg tcgctgtcgc
      361 aggaggtggg cctcgcgctc ggggcgcagg ccggcgaggc gcggctgcgc gaggtggtca
      421 cgggcggcgg gttcagccgg ttccggcggg cgacggagac gcccttcaac atcgtctacg
      481 aggccaagcc gtagggggcc gcgcccgcgt atcgtgaccg cacgcacccg agcacccggt
      541 agtgatactc cgcggtgccg tcgatgcgcg cgccctgccc ggacacacct gagcctgagc
      601 ggtaacggat tatctgtaga cactcccggc aagctcctga gctgcgccga actgttcgtc
      661 cgaaggatga cggctgaacc ctttgagaat cgcctctaaa atcggaagcg tgtgcgagac
      721 gcgcttcgcg cggatgacca tgtcccacag cggctcgaac ttcttccacc cggcggcgta
      781 tgcgaagtgc cgcgccgcct cgagaagggt cggcttcgtc gccgcgccgt cgaggatcga
      841 gcgccaacgg tagaagtcac ggtaagcgcg ccggtagcct tgctccaact tctccggcgt
      901 catgcgggcc gggcggtaga cggcgtggcg cgtgtcgtat aagtcccagt cgtcgtgcaa
      961 catccgcccg gccttcagca tccgttcgta gagcgcggtg ccggggtaag gcgtcaggat
     1021 gtggaacgtc gccgtctcga ttccctgccc gacggcccac tcgaccgtgc gttcgaacac
     1081 gccttcgtcg tcgccgtcca tcccgaagac gaaactgccg ttgaccatca cgccgaggtc
     1141 gtgcagccga cggatggccg ccccgtaatc gcgcccgagg ttgtgcggct tgcgctgctc
     1201 gcgcaggtta tcggcgctca gcgtctcgaa gccgacgaag aggctgcgca gcccgcaagc
     1261 gaccgctttc tccaacaggc ggggacggag caccgactgc accgtcccgg cggcctgcca
     1321 cagtcggttc atcccgcgca tcccgtcgaa gagcgcctcg gcgaaacgcg tgtcgccgaa
     1381 gaggtggtcg tccagaaagt aaagatgacg accgggcagt cgctcgatct cggccaacgc
     1441 gtcgtccacc ttctgcgtgt agaagccgcg cccgccctcg aagaacgctt ccttgtagca
     1501 gaagtcgcac gagtgcgggc acccgcgcga gacgacgatg gagttcggca ccaggtaaag
     1561 gtggcgcttg ataaggtcgc gtcggatgcg cggcagccgg tcgagcgtac gcacggtcga
     1621 gcggtagact ttttgcggac gcccggcgcg gtagtcggag aggaaggcag gccaagtgtc
     1681 ctcgcccggc ccgaggaaga tcgtgtcggc gtgcgccgcc gcttcatcgg gcagcgaggt
     1741 gacgtgcagg ccgccgagca cgacgtaggc gccgcaccgc cggtagtgat cggcgagttc
     1801 gtacgagcgg cgggccgagg tgatgtagac ctgtatcacg accaactcgg gcgcgtcttc
     1861 ggtgtcgagc gtttcgacgt gctcgtcctg aagcgagacc tgtgtgccgt cgtcgagata
     1921 accggccagt gtggcgagac cgagcggcgg gaacagagaa tacttgaccg ggcggaagaa
     1981 ggggcttttc gcttccgtca gtgcgggcag aatcatcttc actttcatcg ccggaacgtc
     2041 actccacctc aaggctactc aggccgccga ttatgcgccc ggacttccgc tttatcaagt
     2101 tctttacacc gtaaagcaac tgcacgaaca atgtggattt ggggtagact gcgcaaggct
     2161 atagaaagtt atcggcagac agtcctggaa atagtgctac ggcgagaacg tcgcaaccac
     2221 ggcgtgtcca aggatgggcg atgagggacg gcaatgattt ccaacatcag ggtttcgctc
     2281 cgcggcttac tgctcgcctg ctgcgcttcg gcggcctgct cgcctctact ccacaccgcg
     2341 tttgcgcagg agggcgccga ccagcagacc cagtcactcg tccggcggct gcaagagggg
     2401 gacacgcagt cccgccgcgc ggctgccgag gagatcaagg ggcgtggggc tgcggcgaaa
     2461 gccgcggtgc ccgcgctggc gctggcgctc aaggacccgg atgatgagtt gcgcaagact
     2521 gcggcggagg ccctcggcag gatggggccg gaggcccgct ccgccgtgcc cgccctgacc
     2581 gaggctctga gagacggcaa cggctcggtg agaagcaacg ccgccttcgc gctcggccaa
     2641 gtcggcgcgg aggccgagtc tgccgtcccg gcactgacag ggctgctgaa agacccgagc
     2701 ccctgggcac gagccggggc ggcccgtgcg ctggggagga tcgggggtca ggcgaaagct
     2761 tccgttcccg cggtagtcga gttgctgaaa gatcggaacg gcggcgtccg cggggacgcc
     2821 gccgaggcgc tcgggcgatt cggcggggac gcgggggccg ccgtccccgc ggtgattgtg
     2881 ttgttgaagg acgagagtgc gtctgtgcgc aaagccgccg ccgaggccct gggaaggatc
     2941 gggccgggcg ccaggggcgc ggtgccggcc ctggccgagt cgatgaagga ccgggagttc
     3001 gtcgtgcgaa tgaacgcggc tcaggcgcta gggcggatcg ggatggaacc ggctgaagcc
     3061 gtcccggtgt ggatgggttc tttaggcgac ccgagcgagc acgtccgcag gtatgccgcc
     3121 gaggcgctgg ccgagatcgg ccccggtgcg agggcggcgg tgccggcgct ggtcggggcg
     3181 ctgaaggatc aggacaggtt cgtccgcctg agcgccgcga aggggctggg gaatatcgcg
     3241 ggcgaggcga gggcagcggt gccggatttg gtcggcttgt tgagggacga ggagccgctg
     3301 gtgcgcgcga acgcggccac ggctttaggc aagctgggcg gcgaggcgag gacggccctg
     3361 cccacgctga acgaactgct caaggattcg gcgacagtcg tgaggctcgc cgcggctgag
     3421 gccatcaaca aaatcgcgtc tcagtgaaca tagccacatt ttgcttctgt ccggcccccc
     3481 aataagggcg cggtggcgta ggtcggaccg tcgatttttt aagctcgttg aagacgaccg
     3541 gtatcgccga ctcaccatcc gtcacgagcg cctgttggcg atgcaccgcg cctccctcca
     3601 cgtcggctgc gtcttgatcc gtcggaacta cctcttgcgg tcgtgaaacc gccactacag
     3661 tatgtcacct gaatggcagg aagagactgc cgacagtgca tctcttcggg aacaactccg
     3721 acatgtctac tggatcggcg gcgggagcgg cgccggcaaa tcgaccgtcg cccgtcgcat
     3781 cgcggcccgg cactccttgc gtgtttacgc gaccgacgac gtgatggcgg atcacgccag
     3841 ccggagcacg agcgaagact cgccgctgct gcacaggttc atggccatgg gcatggacga
     3901 gcggtgggtg aaccgacctc cggacacgat gctcgacacg ttccactggt tccggggcga
     3961 gggcttcgac ctgatcgtcg aagacctcct atccctcccg cgtgagacag gcgtcatcgc
     4021 cgagggtttc cgactgctgc cgcgcctggt cgcgcctctc ctctccgcac ctgcccgtgc
     4081 cgtctggctc ctgccgacgc ccaccatccg tcaggcagtg gtcgagagtc gggggggagc
     4141 ggcctcgggg tttctagcca ggaccaccga cccggaaagg gccttgcgca acctgctcga
     4201 acgcgaccgg atgttcacgg acaagctccg tgaggaagca gcgcgtctgg gcctgagcgt
     4261 cattgatgtg gacggcgcga tgactgagga cgatctcgcc gggcgtgtga cggaagtgtt
     4321 cggcctctat tcaggggctt cattccagca tgagtaatct ccacctctaa caacatgacg
     4381 tcatcgatac caatagcccc cctgggttcg aaaacggctt cactgtggat cacggcgaat
     4441 acgtaggcac aagcttcgaa atgagatgtt tatcaggctg tcttgagagc ggatgtgagc
     4501 ggcgtgaatt gatgtatcct aacgcccttc atgacacacc gattcttagg gaccgtacgt
     4561 agtatattat tctgccctca gcccggctcg gtattattcg tcggtgacct tttccgccca
     4621 ccctttggga gggattgtta accggttgtc cgtttcaagc ggcggggagc cagccatgct
     4681 ttcaaagatc attcgatccg tcttcgtcgg cctaattctg tcagccttca tcgctccagc
     4741 cggtcacgca caaaaagtga cgttaaggat tcagtcggac gtgtccggca ggccgatcag
     4801 tcccgacctt ttcggcatct tcttcgaaga cctcaactac gccgccgacg gcggccttta
     4861 cgcggaactc gtccagaacc gctcgttcga atatcaggct acggaacagc cgacgtggaa
     4921 ttcgctcagc ttttgggagc tgacgaaacg cggagggggc gagggtaagt gggccgtcga
     4981 gtacgcccga ccgctgcaca cgaataaccc gcactacttg gcgctggaag tcactaaagc
     5041 cgccgacggc gtcgggctgg ctaatcccgg cttcgacggc atcgcgctca aagccggcga
     5101 gacgtacgat ctctcgcttt tcgcgcgcca gctcttcatg aaccgtcgct ggccccccga
     5161 caattccatc gaaggcaagc cgatgccgct ggccgtccgg ctggagagta aagacggcga
     5221 agtgttgtcc gaagccccgc tgcaagtcac cggacgcgag tggacgcggc tgaccgctac
     5281 gctgaccccc aaacgggatg aagacgccgc acgcctggtg ctgctggcga aggcgaacgg
     5341 tgggattgcg ttggatgagg tctcactttt cccgcgcaag acgttccgcg accggcccaa
     5401 cgggctgcgc gccgacctcg cgcagacaat cgccgacctg cgcccgaagt tcatccgctt
     5461 ccccggcggc tgcctggtgc acggcaatgg gaccaataac ttctaccgct ggaaggacac
     5521 catcggcccc accgaacagc gtcgcgggca ggcgaacctc tgggggtacc accagagcgt
     5581 cggcctgggt tacttcgagt atttccagtt cgccgaagac atcggggcca agccgctgcc
     5641 cgtcgtggcc gccggcgtct cgtgccagaa ttcggaccac accggcgagg tgggccagca
     5701 atgcctggcc attgcggaca tgcccgccta cattcgagac atcctcgatc tgatcgaatg
     5761 ggcgaacggg ccggccactt ccaggtgggg cgcgaagcgc gcggcggccg gccaccccaa
     5821 gcctttcaac ttcgaatacc tcggcgtcgg caacgaggac gtgatcagcg aggagttccg
     5881 cgtgcgcttc aagatgatcc gcgacgcggt gcgggcgaag catcccgaga tcagggtcgt
     5941 cggcacggtc ggcccgttcc ccgaaggccc ggactacgag gccgggtgga agttcgccaa
     6001 cgagcagcgg ctggagatgg tggacgagca cggctacaag ccgcccgagt ggtactggca
     6061 gaacctctcg cgattcgacg cctacgaccg cacgaagtcg aaggtctacc tcggcgagtt
     6121 cgcggcgcac gaaacgaacc gggcaaacac gctgcggtcg gcactggccg aggcggctta
     6181 catgacctcg ctcgaacgca acggcgatgt cgtccggctg gcctcctacg ctccgctgct
     6241 cgcccgccag gggcacacgc agtggcgccc tgacctgatc tacttcgacg gcgtcaaggt
     6301 tttgcgcacg gcgaactact acgtgcaaca gttgttcagc ctgaaccagg gcgacgccta
     6361 cctcacgaac gcattgacgg gcgtcccccg cccggaccag ttcgcggtgt cgtcggtacg
     6421 cgacggcctg acgggcgaca tcatcatcaa gctggtcaac ggcagccccg cccccctgcc
     6481 gctcctcgtg gaactcaccg gcgcgaaaaa aatctcccgc cgggcgctca agaccgtgct
     6541 ggccggcgag gcggcggcgg tgaacggctt cgacgccccc tcccgcgtag cgccggtcac
     6601 gtccggcatc caagtggaca ggcagttcga atacgaagcg cccgcgcact cgctgacggt
     6661 gatccgcatc gagactcgct gaccgaagtt agtcagcgtc acgttggagg ttcgagtcgc
     6721 ggtcgctcac acgaagaaa
//
LOCUS       DBNGOH010000539         1842 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3929534, whole genome shotgun sequence.
ACCESSION   DBNGOH010000539 DBNGOH010000000
VERSION     DBNGOH010000539.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1842)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1842)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1842
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3929534"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..379
                     /locus_tag="AC159P_10495"
     CDS             <1..379
                     /locus_tag="AC159P_10495"
                     /inference="COORDINATES: protein motif:HMM:NF025910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3695920.1"
                     /translation="VAARARFALAQAREADGKLDEAAALYQELLKDQGKHIPESTVNF
                     RLASIYEKQGKQNEAVEILFRIVEDSRKAAQAKEAKPEREPALVSAAAENLQKLSPER
                     YAKLPAAPVPPRRGFGGGGFPGL"
     gene            469..1599
                     /locus_tag="AC159P_10500"
     CDS             469..1599
                     /locus_tag="AC159P_10500"
                     /inference="COORDINATES: protein motif:HMM:NF016214.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HIR3695921.1"
                     /translation="MDNFTHSLVGLAAAKAGLERVSPYATAVCVLAANLPDADLVTLF
                     AGRYVYIEEHRGVSHSIAGTLALALVFPLIFYGIERLLARVRGREPRAKLKGLLLCSL
                     ALGASHPLLDWTNNYGVRPLLPWDARWLYGDLVFILDPWIWLLLGGAGFLLTAKTARR
                     TSVWAVLGLVLTAAILFLPGQRGMAIPIAARAVWVAGMLLLVIAHRFDVARRWGGAVA
                     AVALLLVVCYWSALSLAHSRAVVRAERVAAQYAAADGARVLKVAAMPMPADPLTWRCA
                     AETGRATYRFDVSLLDADAGARRNERRYVKPGDATAEAVRRAAEDPRARVFLGFSRFP
                     VATARRGAGGETVIQFVDIRFTEPSDRARSDSFTVEIPVNEK"
BASE COUNT          267 a          653 c          609 g          313 t
ORIGIN      
        1 ggtcgcggcg cgcgccaggt tcgcgctcgc gcaggcgcgc gaggccgacg gcaagctcga
       61 cgaggccgcc gcgctctacc aggagctgct caaggatcag ggcaagcaca tccccgagag
      121 caccgtcaac ttccgcctcg cgtccatcta cgagaagcag ggcaagcaga acgaggcggt
      181 cgaaatcctg ttccgcatcg tcgaagactc gcgcaaggcg gcgcaggcca aagaggccaa
      241 gcccgagcgc gagccggccc tcgtcagcgc cgcggccgag aaccttcaga agctcagccc
      301 cgagcgctac gcgaaactcc ccgctgcgcc cgtcccgccg cgccgcggct tcggcggcgg
      361 cggcttcccc ggactctaag aagtcagaag tcaggagtca gaagtcggaa tgaagaaagg
      421 tttattctga cttctgactt ccgacttctg acttcatcat ttctctccgt ggacaacttc
      481 acgcattcgc tcgtcgggct tgccgccgcg aaggcagggc ttgagcgcgt gtcgccctac
      541 gcgaccgccg tctgcgtgct cgccgccaac ctgccggacg cggacttggt cacgctcttc
      601 gccggtcgct atgtctacat cgaagagcac cgcggcgtct cgcactccat cgccggcacg
      661 ctcgcgctcg cgcttgtctt cccgctcatc ttctacggca tcgaacgact gctcgcgcgc
      721 gtgcgcgggc gcgagccgcg ggcgaagttg aaggggctgc tgctctgctc gctcgcgctc
      781 ggcgcgtcgc acccgctgct cgactggacg aacaactacg gcgtgcgccc gctcctgccg
      841 tgggacgcgc gctggctcta cggcgacctc gtcttcatcc tcgacccgtg gatatggctg
      901 ctgctcggcg gcgcagggtt tctgctgacg gcgaagacgg cgcggaggac ttccgtctgg
      961 gccgtcctcg gattggtgct gacggcggcg atcctcttcc tgccagggca gaggggcatg
     1021 gcgatcccga tagccgcgcg cgccgtgtgg gtggcgggca tgctgcttct cgtcatcgcg
     1081 caccgtttcg acgtggcgcg gcgttggggc ggggccgtcg ccgcggtcgc gctgctgctc
     1141 gtggtctgct actggagcgc gctctcgctc gcgcactcgc gcgccgtcgt gcgagcggag
     1201 cgcgtcgccg cgcagtacgc cgccgccgac ggcgcgcgag tgctcaaggt cgcggcgatg
     1261 ccgatgccgg cagaccctct gacgtggcgc tgcgcggcgg agaccgggcg cgcgacctac
     1321 cgcttcgacg tgtcgctgct cgatgcggac gcgggcgcgc ggcgcaacga gcggcgctac
     1381 gtcaagccgg gggatgcgac ggcggaagcc gtccgccgcg ccgccgaaga cccgcgcgcg
     1441 cgcgtcttcc tcggtttctc gcgctttccg gtggcgaccg cccgccgcgg cgcgggcggc
     1501 gaaaccgtca tccagttcgt ggacatccgc ttcaccgaac cgagcgaccg cgcgcgcagc
     1561 gacagcttca cggtcgagat tcccgtcaac gaaaagtaag cccggagtcc gacgctcgga
     1621 gtgggaagcc tcgctcttga tgtcgaacct tgagccgcga actttgaact atccctgtcc
     1681 gcgccccttc tgttatcttc ttccgcgcgt tctcgccgac cgggttttcg cacggcgcgt
     1741 tttggctcac ctcgaaagga ctgaacggaa ccacatgaaa agaaccttcc ttacctcgct
     1801 ggcgctcgcc gcgtgcatgg tcttcgcgct cgcgccgcag gc
//
LOCUS       DBNGOH010000540         2248 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4844737, whole genome shotgun sequence.
ACCESSION   DBNGOH010000540 DBNGOH010000000
VERSION     DBNGOH010000540.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2248)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2248)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2248
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4844737"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..920)
                     /locus_tag="AC159P_10505"
     CDS             complement(<1..920)
                     /locus_tag="AC159P_10505"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HIR3695922.1"
                     /translation="MRKIVSAAEMREIDRLTTERYATPSLLLMEAAANAAARAVASKF
                     SSDLTNKTVQVLCGRGNNGGDGAALARALWMMGAHVDVLLFGKVEDARGDARTNFEIV
                     RRLASFEAGSKERPSRVSFVECDRIDAWEKIASARRGYDVIVDALFGTGLKRPLEGVF
                     TQVVAHLELLRAAREHAGLERPLFVSLDLPSGLDADSPAPVGDAVRADLTVTFTAPKQ
                     ANVLPPASHFGGTLVVADIGSPPALLDAAPSRLFLTEAADARAWLEATRYAPDSYKNT
                     HGHALVVAGSRGMTGAAALCGNAAMGAGAGL"
     gene            complement(1271..1984)
                     /locus_tag="AC159P_10510"
     CDS             complement(1271..1984)
                     /locus_tag="AC159P_10510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_352431723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HIR3695923.1"
                     /translation="MTNRIFAMAKPFALLAFAFAIFTLGQPQARADEVFIAGFTNGCF
                     GAGCTPGASASIPGLTYSNSTFSGTTAGGFRGLGGNPNPGANVNNLGSFTLSTAPNTF
                     NTSFSLQVTFTAPQGINGSNTAIFAATVTGMVRAADDGGVFIDFDNTLILFTFVDTNC
                     GATTVPGQQTTCGTGSFFFSVNDVAIDPGQTASVTGQITSAQQQSAVPEPATMLLLGT
                     GIAGIAARVRRRRDKTAQQ"
BASE COUNT          388 a          685 c          792 g          383 t
ORIGIN      
        1 agtcccgcgc ccgcgcccat cgcggcgttg ccgcacagcg ccgccgcgcc cgtcatgccg
       61 cgcgagccgg cgacgacgag cgcgtggccg tgcgtgttct tgtaggagtc gggcgcgtag
      121 cgcgtcgctt cgagccacgc gcgcgcgtcc gcggcttcgg tcagaaaaag tctggacggc
      181 gcggcgtcga gcagcgcggg cggcgagccg atgtccgcga cgacgagcgt gccgccgaag
      241 tgcgaggcgg gcggcaggac gttggcttgc ttcggggccg tgaaggtgac ggtgaggtct
      301 gcgcgcacgg cgtcgccgac gggcgcgggc gagtcggcgt cgagacccga ggggaggtcg
      361 agcgagacga agagcggtcg ctcaagcccc gcgtgctcgc gcgccgcgcg caggagttcg
      421 aggtgcgcga cgacctgcgt gaagacgcct tcgagcggtc gcttgagtcc cgtgccgaag
      481 agggcgtcca cgatcacgtc gtagccgcgc cgcgcgctcg cgatcttctc ccacgcgtct
      541 atccggtcgc actcgacgaa ggagacccgc gagggccgct ctttcgaccc cgcctcgaaa
      601 ctcgcgaggc ggcggacgat ctcgaagttg gtgcgggcgt cgccccgtgc gtcctcgacc
      661 ttcccgaaga ggagcacgtc cacgtgtgcc cccatcatcc agagcgcgcg cgcgagcgcc
      721 gcgccgtcgc cgccgttgtt gccgcgcccg cagagcacct gcacggtctt gttcgtcaaa
      781 tcggaagaaa actttgaggc gacggcgcgg gccgcggcgt tggcggcggc ctccatcagc
      841 agcagcgagg gcgtggcgta ccgctccgtg gtgagacggt cgatctctcg catctcggcg
      901 gcggaaacga ttttgcgcac gggtgcgatg ataaagagag acccttcggc ggtcaaaccc
      961 ggtttcgtgg aatcggcgcg ggagagttcc cggaatgggt gcgaagcgaa gacggaaaag
     1021 tctgtcgggg cggcaataaa aaaaggcgcc gtttcagcgc ccctctcgcc acttgcctgc
     1081 cgctacgccc gcaccgtccg tcgtttgtat accacaggaa atgtttaatc aggttgtccc
     1141 tccagaaaaa aagagcggcg cgaaaaatat ttcgacgatg agggaagagc cgcgccgaaa
     1201 ctcgtccggc gcggcccttc tttcgagtcg gataggtttg cgggaaggag gaacctcaac
     1261 ccgctagccg ttattgctgt gcggttttgt cgcggcggcg gcggacgcga gcggcgatgc
     1321 ccgcgatgcc tgtgccgagt aggagcatcg tcgccggctc ggggacggcg gactgctgct
     1381 gcgcgctcgt gatctgcccg gtgaccgaag cggtctgtcc ggggtcgatc gccacatcgt
     1441 tcaccgagaa gaagaacgag ccggtcccgc aggtcgtctg ctgccccggt acggtcgtcg
     1501 ccccgcagtt cgtgtccacg aaggtgaaca ggatcagcgt gttgtcgaag tcgatgaaca
     1561 cgccgccgtc gtccgccgcc ctgaccatgc ccgtgacggt cgcggcgaag atcgccgtgt
     1621 tggacccgtt gatgccctgc ggggccgtga acgtcacttg cagcgagaac gacgtgttga
     1681 aggtattcgg ggcggtgctg agcgtgaacg agccgaggtt attgacgttc gcgcccgggt
     1741 tcgggttgcc gccgaggccg cggaagccgc cggcggtggt gcccgagaag gtcgagttgc
     1801 tgtaagtgag accggggatc gaggccgagg cgccgggcgt gcagcccgcg ccgaaacagc
     1861 cgttggtgaa cccggcgatg aagacctcgt cggcgcgcgc ttgcggctgt cctaaagtga
     1921 agatggcgaa ggcaaaggcg agtagggcaa aaggcttagc catggcgaag atgcggttgg
     1981 tcatcgatgt tcctccgtcg ttcattgtga atctttgggg ttgtacttat cacgcactcc
     2041 gcatccctcc ccgctttagc ctttaaaggg agaggtgcaa ggctcttcag tccgatgccc
     2101 tcggaggtgg cggtgaaccg cacgaacacc tgctcctctt caagttgaaa agacggaaag
     2161 ccttgtcttt agccgattgc ggcagccgac aggggcaact gagggccgcc taaggatttc
     2221 aactctctgg actcgtagag ccagtaaa
//
LOCUS       DBNGOH010000541         5605 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1729797, whole genome shotgun sequence.
ACCESSION   DBNGOH010000541 DBNGOH010000000
VERSION     DBNGOH010000541.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5605)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5605)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5605
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1729797"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..572
                     /locus_tag="AC159P_10515"
     CDS             <1..572
                     /locus_tag="AC159P_10515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695924.1"
                     /translation="SLLALVYVFSEASRARAEADVAEQQTMRLRAEKAALEDRAKQIT
                     QEIPEDQRATLRAAHEIVDRKTFSWSQLFSDLEESLPSSVRVSRINVRDVTQRGGLTR
                     AELDMALVGRTPTDVTGMIAEMARGGTFSAIPLTENQKSGKGESGYEWTLRVSYTQRA
                     RGKSNEGAGANSVAAAARAEETDDDGGRR"
     gene            576..1460
                     /locus_tag="AC159P_10520"
     CDS             576..1460
                     /locus_tag="AC159P_10520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695925.1"
                     /translation="MKDETTVISKRAGAGGARRMGGRLPQLNRGARATGPLGLPEIIA
                     LAAAALLLVTAIASYLLLLRPQRTRLQAMNEERALLQKQLQDMESTVGKNQDAQASAR
                     TILDSLVGFETEHLGDGGQGSTRVIEELNRLIRKNGLRISGGLAFTQLQETAPGEAQR
                     RQQRGGTGEQSAARVVESVFPGIAVTLTVEGTYPKLRQFIRDVESDRQSFIVVNAVEL
                     EGIADSGGPSAAAADAPVVSTGEEMPGAAPRPTAAPSGVRGTVVSLRLEMAAYFRRAN
                     VTAPDEQPAPTRPDGPAR"
     gene            1481..2404
                     /locus_tag="AC159P_10525"
     CDS             1481..2404
                     /locus_tag="AC159P_10525"
                     /inference="COORDINATES: protein motif:HMM:NF013955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HIR3695926.1"
                     /translation="MALFEGKTPAERNKMIAAAVLPLLALIFVVRMFFSSPAPSRPAS
                     NSNNKANPNRNAPRANPNATDLPPSVAEVPTPVVYNPFPFGGANAGRNIFAYYVKPEG
                     TATVKETPTPPPPTPMPTPPLLLSSLSPASVYARTSGFTMQVSGDKFTPESRVYVEGQ
                     EVPTQFRSPQQLTATVGAAALSAPGARPVVVRTPDNILFSNGATLNVMQPPAPTHTYV
                     GMIGRQRVATGLLKNQKGELVSVQPGDIVEGRFRVAEISERAVDLVDKDLNIRHTLPY
                     VESRTTGQTGRIPGSIQPPPPPKPDDGDEEP"
     gene            2433..3116
                     /locus_tag="AC159P_10530"
     CDS             2433..3116
                     /locus_tag="AC159P_10530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly FimT family protein"
                     /protein_id="HIR3695927.1"
                     /translation="MNGQRASIHRQHTTTRTWSRQRRARERGYALVALLALMTVLAIS
                     MMAVAPSIRQQSRRELEKEAIARGEEVAEAIRKWIHYKGTPPTSMDDLVEGVNPPGLP
                     KKIQLLRASAARDPLTKDGTWRTIKPNDQELRDFVVAITRYANSQTPPTNDRHPAMTT
                     IAATLPREIRGLVNLDEEEEDAPCDEKASDTSSGPFVGVASRSRCASVINYYGIGRHD
                     KWVFTPFYR"
     gene            3730..4209
                     /locus_tag="AC159P_10535"
     CDS             3730..4209
                     /locus_tag="AC159P_10535"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0141098 - tRNA
                     (cytidine(34)-2'-O-ribose)-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HIR3695928.1"
                     /translation="MISVALVEPEIPPNTGNVARLCAATHTPLHIVGATGFRLDDRAV
                     RRAGLDYWPEVRLARHRDMDALRAALAPETRFVYLTTKAEVSYLDRAFGADDCLVFGR
                     ETRGLPEELLRANWPDCVTIPMPNPKVRSLNLATAVAVVLYEALRQTGALKAVNRQS"
     gene            4308..5576
                     /locus_tag="AC159P_10540"
                     /pseudo
     CDS             4308..5576
                     /locus_tag="AC159P_10540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012458948.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
BASE COUNT         1066 a         1963 c         1772 g          804 t
ORIGIN      
        1 cctcgctgct cgcgctcgtc tacgtcttct ccgaggccag ccgcgcgcgc gccgaggccg
       61 acgtggccga gcagcagacg atgcgcctgc gcgccgaaaa ggccgcgctc gaagacaggg
      121 ccaagcagat cacgcaggag attcccgagg atcagcgcgc gacgctcagg gccgcgcacg
      181 agatcgttga ccgcaagacc ttctcgtggt cgcagctctt ctcagacctc gaagagtccc
      241 tgccgtcgag cgtccgcgtc tcgcgcatca acgtgcgcga cgtgacgcag cgcggcgggc
      301 tgacgcgcgc cgaactcgac atggccctcg tcggcaggac gccgaccgac gtgaccggca
      361 tgatcgccga gatggcgcgc ggcggaacct tctccgccat cccgctcacg gagaaccaga
      421 agtcgggcaa gggcgagagc ggctacgagt ggacgctgcg cgtgagctac acgcagcgcg
      481 cgcgggggaa gtcgaacgag ggcgcgggcg ccaacagcgt cgccgccgcg gcgcgcgccg
      541 aagagacgga cgacgacggg gggcggcggt aaatcgtgaa ggacgagacg acggtcatca
      601 gtaagcgcgc gggcgcgggc ggcgcgcggc gcatgggcgg gcggctcccg cagctcaacc
      661 gcggcgcgcg ggccacgggg ccgctgggcc tgcccgagat catcgcgctc gccgccgccg
      721 cgctgctgct cgtcacggcc atcgcctcct acctcctcct gctccgcccg cagcgcacgc
      781 gcctccaggc gatgaacgaa gagcgcgcgc tcctccagaa gcagttgcag gacatggaat
      841 cgaccgtcgg caagaatcag gacgcgcagg cgagcgcgcg caccatcctc gacagcctcg
      901 tcggcttcga gaccgagcac ctcggcgacg gcggacaggg gagcacgcgc gtcatcgaag
      961 agctgaaccg cctgatccgc aagaacggcc tgcgcatcag cggcggcctc gccttcacgc
     1021 agttgcagga gaccgcgccc ggcgaggcgc agcggcggca gcagcgcggc ggcaccggcg
     1081 agcagtcggc ggcgcgcgtc gtcgagagcg tcttccccgg catcgccgtc acgctcacgg
     1141 tcgaaggcac ctacccgaag ttgcgccagt tcatccgcga cgtggagtcc gaccgccagt
     1201 cgttcatcgt cgtcaacgcc gtcgagctcg aaggcatcgc cgactcgggc ggcccttcgg
     1261 cggcggcggc ggacgcgccc gtcgtctcga ccggcgaaga gatgcccggc gccgcgcccc
     1321 gccccacagc ggcgccgagc ggcgtgcgcg gcacggtcgt cagcctgcgg ctcgaaatgg
     1381 ccgcctactt ccgtcgcgcg aacgtcacgg cgcccgacga gcagcccgcg ccgacgcggc
     1441 ccgacggccc ggcgcgctga agcagtaaag cacgaagcac atggctctat tcgagggtaa
     1501 gacccctgcg gaacgcaaca agatgatcgc ggcggcggtg ctcccgctcc tcgcgctcat
     1561 cttcgtcgtc cgcatgttct tcagctcgcc cgcgccgagc cgcccggcct ccaacagcaa
     1621 caacaaggcg aacccgaacc gcaacgcgcc gcgcgccaac ccgaacgcga cagacctgcc
     1681 gccgtcggtc gccgaagtgc cgacgccggt cgtctacaac cccttcccct tcggcggcgc
     1741 caacgcgggc cgcaacatct tcgcctacta cgtcaagccg gaagggacgg cgacggtcaa
     1801 ggagacgccg accccgccgc cgcccacgcc catgccgacg ccgccgctgc tgctgagcag
     1861 cctctcgccc gcgagcgtct acgcgcgcac cagcggcttc acgatgcagg tctccggcga
     1921 caagttcacg cccgagtcgc gcgtctacgt cgaagggcag gaggtgccga cccagttccg
     1981 cagcccgcag caactcaccg cgaccgtggg cgccgccgcg ctctcggcgc cgggcgcgcg
     2041 ccccgtcgtc gtccgcacgc ccgacaacat cctcttctcg aacggcgcca cgctcaacgt
     2101 catgcagccg cccgcgccga cccacaccta cgtcggcatg atcgggcgcc agcgcgtcgc
     2161 caccgggctg ctcaagaacc agaagggcga actcgtcagc gtccagccgg gcgacatcgt
     2221 cgaggggcgc ttccgcgtcg ccgaaatctc cgagcgcgcc gtcgatctcg tcgataagga
     2281 cttgaacatc cgccacacgc tgccctacgt cgagtcgcgc acgaccggcc agacgggacg
     2341 catccccggc tccatccagc ccccgccccc gcccaagccc gacgacggcg acgaggagcc
     2401 gtaacagcac ctccttcatc atcgcaccag ccatgaacgg acagcgcgcg agtattcatc
     2461 ggcaacacac gacgactcga acgtggtcgc ggcagagacg cgcgcgcgaa cgcggctacg
     2521 cgctcgtcgc gctgctcgcg ctgatgacgg tgctcgccat ctcgatgatg gccgtcgcgc
     2581 cgagcattcg ccagcagagc cgccgcgagc ttgagaagga ggccatcgcg cgcggcgagg
     2641 aggtcgccga ggccatccgc aagtggattc actacaaggg cacgccgccg acctcgatgg
     2701 acgacctcgt cgaaggcgtc aacccgccgg gcctgccgaa gaagattcaa ctcctgcgcg
     2761 ccagcgccgc gcgcgacccg ctcacgaagg acggcacgtg gcgcaccatc aagcccaacg
     2821 accaggagct gcgcgacttc gtcgtcgcca tcacgcgcta cgccaacagc cagacgccgc
     2881 cgaccaacga ccgtcacccc gccatgacga ccatcgccgc cacgctcccg cgcgagatac
     2941 gcggactcgt caacctcgac gaagaagagg aggacgcgcc gtgcgacgag aaggcgtccg
     3001 acacgagcag cggccccttc gtcggcgtcg ccagccgcag ccgttgcgcg tccgtcatca
     3061 actactacgg catcggtcgc cacgacaagt gggtcttcac tcccttctac cgttagtccg
     3121 catccgcaac ccccgtcacg cctcgatcga catcgcacgc ctccgcgaaa gtcttgcgcg
     3181 cgcatcatgc gatgcacacg cgcgaaattt ttttcgcatt acttaattaa gttgttgaca
     3241 tccatgttta ttgtgtttat atactcaaca tgttgtgcgc cattcactct agaccacaag
     3301 aaaaaaactg acacagtatc ttgtggtcag tcacaactca aaacgaaaag gagcagctac
     3361 agcttatggc aactaagaaa gcagctaagg gcggcgcgaa aaaaggcgca gcgaaaaagg
     3421 gcggcggcgc gaaaaaagct tcgaagaagt ccggcgccaa gaagcggtaa ctcagcagca
     3481 gccaggggta gtagtcacgt caaccgaaac cggcgcacac aactttctgc gaccctagtg
     3541 gtcttagcca aggacggcga ttgcatcacg atgcgacgcc gtccttcttc tttcggctct
     3601 tagcggttgg ctcttggctt tcaacttttg tcggtcggat gagtcgcgcg ttcacatgcg
     3661 acctctcggc ccgcggaaga atgtgagctg ccagcaaaga gctaacagct aaaggctaac
     3721 agcttcccca tgatttccgt cgcgctcgtc gagccggaga taccgcccaa cacgggcaac
     3781 gtcgcgcgcc tgtgcgcggc gacgcacacg cctttgcaca tcgtcggcgc gacgggtttc
     3841 cgcctcgacg accgcgccgt gcgccgcgcg gggttagact actggcccga agtccggctc
     3901 gcgcgacacc gcgacatgga cgcgctgcgc gcggcgctcg cgcccgagac gcgcttcgtc
     3961 tacctcacga cgaaggccga ggtctcctac ctcgaccggg ccttcggcgc ggacgattgc
     4021 ctcgtttttg gccgggaaac gcgcggtttg ccggaggagc tgctgcgtgc caactggccc
     4081 gactgcgtga ccatcccgat gcccaacccg aaggtgcgga gcctcaacct cgccaccgcc
     4141 gtcgccgtcg tgctctacga agcgctgcgc cagacggggg ccttgaaggc cgtcaaccgt
     4201 caatcgtaaa tcgtgaccgg tggcaagtct ccggcgcatg ctttaagctg ttgccgattg
     4261 atgactcacg gttcacggtt cacggcttac ggttcacggt tttttttatg aacagagaag
     4321 acatccgcaa catcgccatc atcgcgcacg tcgatcacgg gaagaccacg ctggtcgatg
     4381 ccatgctcaa ccagtcgggc acgtggcgcg acaacgagca gcacgtcgag cgcgtgatgg
     4441 actcgatgga cctggagcgc gagcgcggca tcacgatcat ggcgaagaac acctcggtgc
     4501 gcttcggcga cgtgaagatc aacatcgtgg acacgccggg ccacgccgac ttcggcggcg
     4561 aggtcgaacg cgtgctgaag atggtggacg gcgtgatgct tttggtggac gcggcggaag
     4621 ggccgctgcc gcagacgcgc ttcgttttgc gcaaggcgct cgaagcgcac ctgccggcca
     4681 tcatcgtcgt caacaagatc gaccggcatg acgcgcgacc cgaggaggtg gtcaacgaaa
     4741 tctacgacct cttcatcgac ctcgacgcga ccgacgagca gatcgacttc cccatcctct
     4801 acaccgtctc gcgcgacggc gtggccaaga agaacctcga agacggctcg caaagcttgc
     4861 gtcctctctt cgagcagatc gtcgagacca tcccgccgcc gaaggagtta aggcaggacg
     4921 cgttgcaact gctggtcgcc aacctcgact acaacgagta cgtgggccgg ctcgccatcg
     4981 gcagaatctt ttcgggcgag gtggcggcgg gcgacacggt cgccgtcgtc aggcgcgacc
     5041 gctcggtcac gaagacgcgc gtcaaggagc tgtacgcctt cgaggggctg aagcgcgagt
     5101 cgataacgag cgcgcgcttc ggcgagatcg tcgcgctcgc gggcgtcgag ggcatcgaga
     5161 tcggcgagac gttgacgagt gccgacaacc cgaaagggct tccgaccatc gccgtggacg
     5221 agccgacgat ctcgatggtc ttcggcgtca acaactcccc gttcgcgggc accgacggga
     5281 agttcgtcac gtcgcgccag atcaaggagc ggctcgaccg cgagctgctg agcaacgtcg
     5341 ccatccgcgt cgaggcgacg gactcgcccg accagttcaa ggtctcgggc cgcggcgagt
     5401 tgcagctcgc catcctcatc gagatgatgc ggcgcgaggg ctacgagcta caggtctcga
     5461 agccggaggt catcacgcgc gcggagaacg gcgtgacgct cgaacccatc gagcaggtcg
     5521 tcgtcgattg cccggacgaa ttcatcggtg tcgtgacgga agctttgggc cgccgctagg
     5581 ggcagatgac gaagatggtc atcca
//
LOCUS       DBNGOH010000542         4578 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1180772, whole genome shotgun sequence.
ACCESSION   DBNGOH010000542 DBNGOH010000000
VERSION     DBNGOH010000542.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4578)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4578)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4578
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1180772"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(418..1566)
                     /locus_tag="AC159P_10545"
     CDS             complement(418..1566)
                     /locus_tag="AC159P_10545"
                     /inference="COORDINATES: protein motif:HMM:NF012318.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HIR3695929.1"
                     /translation="MLKRKVPLLLALVLFPLLTARAAAAEESLPELVRRVKPSVVSVI
                     TYDQKGEVKITGSGFFVRPGQILTNLHVIEGASRAEVRTFEGKGRTYAVEGLLAVDAE
                     GDLALLDVSSAQERSRAVETAAALPEEGEKIFVIGNPLRLEGSVSDGIVSAVREVPNL
                     GKIIQITAPISHGNSGSPVFNMKGQVVGIVTIRVMNGQNINLALGAGRAAELGAGERL
                     LSFEEVAKTKSTLKAESLADWSYLNGLNSLWLGNYDSALGHFENAVNKNPERADAWIQ
                     VGFCKVKQGKNQDAIRAFQQALRLRPNSYEAHNKLGDAYYFAGNYRKAADSYRQAVAL
                     RPDLAEGYYNLGMAQFELGDRAAAAAQAQRLKTLDAELHKKLAGELAR"
     gene            1872..3212
                     /locus_tag="AC159P_10550"
     CDS             1872..3212
                     /locus_tag="AC159P_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008097416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BNR repeat-containing protein"
                     /protein_id="HIR3695930.1"
                     /translation="MKWTHPVALFGVAATVTALSLTSATLSAQSPTRDGARTFPIAEG
                     WAANQVNAVIFRRSSVTTHGDTQFVAFYDAEARVVLAKRKPGTARWEVRRTEYEGDAR
                     DAHKSISIAVDGDGFLHAAWNMHDTPLLYRRSLGPGSLELARETPMRGDREGRVTYPE
                     FYNLPGGDLLFLYRDGASGGGNLVLNRYDVKAKRWSRLQDNLLGGEGRRNAYWQLAVD
                     ARGAIHLSWVWRETPDVLTNHDLCYAKSADGGRTWRKSSGEKYRLPITAASAEYVLRI
                     PQGSELINQTSMAADAAGRPYIASYWRPPGTKVPQYHLVYHDGRRWLTSQVTRRTTAF
                     SLSGRGTRRIPVSRPQVLVDARRDKARVVVVFRDIERGERVSVAVCEDVRRGAWAIKD
                     LTEESVGMWEPTYDAAMWDRGKELHLFVQRVGQGQGEGVEDIPPQMVSVLEWKP"
     gene            3355..>4578
                     /locus_tag="AC159P_10555"
     CDS             3355..>4578
                     /locus_tag="AC159P_10555"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013469.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 35 protein"
                     /protein_id="HIR3695931.1"
                     /translation="MLKTKLFMSAALVVLALFAPAYARRSDAASARAAHTFGWRGEHF
                     LLDGKPFKIIAGDMHYARVPREYWRDRMRKMKAMGLNTLTTYVFWNLHEPQPGRFDFG
                     GNLDIAAYVRTAQEEGLWVIVRPGPYVCSEWEFGGFPSWLLKEPDMKVRSADPRFVKA
                     AANYMKEVGRRLAPLQITRGGPVIMVQVENEYGSFGSDEVYMNAVRRMIVDAGFEVTL
                     FTSDGDAGKLAEGTLPDVLAVINFGARESPEKKFAVFDKFRQNVPRMCGEFWVGWFDS
                     WGDRHQTVPAERAAEGLDWMLARGISVSIYMFHGGSSFGFMNGANRNNEGYKPIINGY
                     DYDSPLDEAGRPTPKFFALRDVIKKHLPAGTVLPELPAPLPLVEVPRFELKESASLFS
                     AAGRAARAERPLPMEA"
BASE COUNT          768 a         1444 c         1551 g          815 t
ORIGIN      
        1 tcgcttgcgg cggcgccgcc tttgagaatc accaccccac agtttccgaa attcaacact
       61 tccgttccgt gtgggccgga tggctgtgtg agaccaaacg gaagcaaaag ggcgtgtcag
      121 gtgacacgtc cgccgactac gttccgaaga cttgaagcat gccgcaaagc gctcccaagc
      181 ctaatcctac ggctgtgcta tgtcaataaa aattgtggcg ggtgcgtagt caaggaaagc
      241 gggcagcggg cagcgggcag caggcggtag gcagtaaaag caggaggctt gagacgatga
      301 atctcaagcc tcctgctctg atcgctcgaa gagagaagag gctcgggact ttacctccca
      361 agcctcttcg cctttctatc ttgaactgcc taccgcctac tgcctactgc tttcttctca
      421 ccgcgccagc tcgcccgcga gcttcttgtg cagctcggcg tcgagcgtct tgaggcgctg
      481 ggcctgcgcg gcggcggcgg cgcggtcgcc gagttcgaac tgcgccatgc cgaggttgta
      541 gtagccctcg gcgaggtctg ggcgcagggc gacggcctgc cggtaggagt cggcggcctt
      601 gcggtagttg cccgcgaagt agtaggcgtc gccgagcttg ttgtgcgcct cgtaggagtt
      661 agggcgcagg cggagcgcct gctggaaggc gcggatggcg tcctgattct tgccttgctt
      721 gaccttgcag aagccgactt gaatccacgc gtcggcgcgc tcagggttct tgttgaccgc
      781 gttctcgaag tggccgagcg cgctgtcgta gttgccgagc cagagcgagt tcagcccgtt
      841 caggtacgac cagtcggcga gcgattcggc cttcaaagtg ctcttggtct tcgccacctc
      901 ctcgaagctc agcagccgct cgcccgcgcc cagctccgcc gcgcgcccgg cgccgagcgc
      961 gaggttgatg ttctgcccgt tcatcacgcg gatggtgacg atgccgacca cctgcccctt
     1021 catgttgaag acggggctgc ccgagttgcc gtgcgagatg ggcgccgtga tctggatgat
     1081 cttgccgagg ttcggcacct cgcgcaccgc cgagacgatg ccgtccgaga ccgagccttc
     1141 gagcctcaag gggttgccga tgacgaaaat cttctcgccc tcttccggca gcgccgccgc
     1201 ggtctcgacc gcgcgcgagc gctcctgcgc gctgctcacg tcgaggagcg cgaggtcgcc
     1261 ctcggcgtcc accgccagca gaccctcgac cgcgtaagtg cggcccttgc cctcgaacgt
     1321 cctgacctcg gcgcggctcg cgccttcgat cacgtgcagg ttcgtcagga tttgtccggg
     1381 gcggacgaag aagccgctgc ccgtgatctt gacctcgccc ttctggtcgt aggtgatgac
     1441 ggagacgacc gacggcttga cccgccggac gagttcgggc agagactctt ctgccgccgc
     1501 ggcgcgcgct gtcaaaaggg ggaacagcac gagcgctagc aagaggggga ccttgcgttt
     1561 caacatctcg acttctcttt caagtgattg agccgctttg tggggcggcc ccgcggggtg
     1621 ggacaaaccc tctgacaacc tcgacgcccg gtcgggagac agcgtcccga cgattatgct
     1681 tcaaaaaagc cggaagtcaa tcattttgtt tcgaaattgt gtcgggcggt gaagagggtg
     1741 ctaatatgcc cgtaaatcga gggatttcgg gttcgcgcct ttttcagtgc gaggcgatga
     1801 aatccgccgg gggtttcttc ggcggccttc gtgtgtcatc gtgtggcgac ggacgaggtg
     1861 gaagaggatt gatgaagtgg acgcaccctg tggctctctt cggcgtggcc gcgacggtca
     1921 ccgcgctttc gctgacgagc gcgaccctct cggcgcagtc gccgacgcga gacggcgccc
     1981 gcaccttccc cattgccgaa gggtgggccg cgaatcaggt caacgccgtc atcttccggc
     2041 gcagctccgt gaccacgcac ggcgacacgc agttcgtcgc cttctacgac gcggaggcgc
     2101 gcgtcgtgct ggcgaagcgt aagccgggca cggcccgttg ggaggttcgg cgcaccgagt
     2161 acgagggcga cgcgcgcgac gcgcacaagt cgatcagcat cgcggtggac ggcgacggct
     2221 ttctgcacgc cgcctggaac atgcacgaca cgccgctgct ctaccgccgc tcgctcggcc
     2281 ccggctcgct cgaactcgcg cgcgagacgc cgatgcgcgg cgacagggaa gggcgcgtga
     2341 cctacccgga gttttacaac ctgcccggcg gcgacctgct gttcctctac cgcgacggcg
     2401 cctcgggcgg cggcaacctc gtgctcaacc gctacgacgt gaaggcgaag aggtggtcgc
     2461 ggcttcagga caacctcctc ggcggcgagg ggcgccgcaa cgcctactgg cagttggcgg
     2521 ttgacgcacg gggcgcgatc catctttcgt gggtgtggcg cgagacgccc gacgtgttga
     2581 ccaaccacga cctctgctac gcgaagtccg cggacggcgg caggacgtgg cggaagtctt
     2641 cgggcgagaa gtatcggctg cccatcaccg cggcgtccgc agagtacgtg ctccgcatcc
     2701 cgcagggcag cgagctgatc aaccagacct cgatggcggc ggacgccgcg ggccgtccct
     2761 acatcgcctc ttactggcga ccgccgggga cgaaggtgcc gcagtatcat ctcgtctacc
     2821 acgacggcag gcgttggctc acgtctcagg tcacgcgccg cacgaccgcc ttcagcctgt
     2881 cgggtcgcgg cacgcgccgc atccccgttt cgaggccgca agtcctcgtc gatgcgaggc
     2941 gggacaaggc gcgggtcgtc gtcgtcttcc gcgacatcga gcgcggcgag cgcgtctcgg
     3001 tcgccgtctg cgaggacgtt cggcgtgggg cgtgggcgat caaggatttg accgaagagt
     3061 ccgtcgggat gtgggagccg acatacgacg cggcgatgtg ggacagaggg aaggagctgc
     3121 acctcttcgt gcagagggtc gggcaggggc agggcgaagg cgtcgaggac atcccgccgc
     3181 agatggtctc cgtccttgaa tggaagccgt gacaatgaag catctgggga aaaggtcaaa
     3241 agggggagag gcgaaaaggg agaagaagcg acttgctttc tacctctccg ccttttgccc
     3301 ttttaacctt ttcccctctt ccccttttcc cctttcttcg agaagatgaa tctcatgctc
     3361 aagacaaaac tcttcatgtc ggcggcgttg gttgtgctgg ctctcttcgc gccggcttac
     3421 gcgcgaagga gcgacgcggc gagcgcgagg gccgcgcaca cgttcgggtg gcggggcgag
     3481 cacttcctgc tcgacggcaa gccgttcaag atcatcgcgg gcgacatgca ctacgcgcgc
     3541 gtgccgcgcg agtactggcg cgaccggatg cggaagatga aggcgatggg gctgaacacg
     3601 ctgaccacct acgtcttctg gaacctgcac gagccgcagc cgggccgctt cgatttcggc
     3661 ggcaacctcg acatcgcggc ttacgtccgc acggcgcagg aggagggctt gtgggtcatc
     3721 gtgcggcccg gcccgtacgt ctgctcggag tgggagttcg gcggcttccc ctcttggttg
     3781 ttgaaggagc cggacatgaa agtccgcagc gccgacccgc gcttcgttaa ggctgcggcg
     3841 aactacatga aggaggtcgg gcgcaggctc gcgccgctgc aaatcacgcg cggcggcccg
     3901 gtcatcatgg tgcaggtcga gaacgagtac ggctcgttcg gcagcgacga agtctatatg
     3961 aacgccgtgc gccggatgat cgtggacgcc ggtttcgagg tgacgctgtt cacgtcggac
     4021 ggcgacgcgg gcaagctcgc cgaagggact ctgccggacg tgctcgcggt catcaacttc
     4081 ggcgcgcgcg agtcgccgga gaagaagttc gccgtcttcg acaagttccg ccagaacgtc
     4141 ccccgcatgt gcggcgagtt ctgggtcggg tggttcgact cgtggggcga ccggcaccag
     4201 accgtcccgg cggagagggc tgccgagggg ctggactgga tgctcgcgcg cggcatctcg
     4261 gtcagcatct acatgttcca cggcggctct tcgttcggct tcatgaacgg ggcgaaccgg
     4321 aacaacgaag gctacaagcc gatcatcaac ggctacgact acgactcgcc gctcgacgag
     4381 gcgggccgcc cgaccccgaa gttcttcgcg ctgcgcgacg tgatcaaaaa acacctgccc
     4441 gcgggaacgg tgctgccgga gctgcccgcg ccgctgccgt tggtcgaggt gccgcgcttc
     4501 gagctgaagg agtcggcgag cctcttctcc gcggcgggac gcgccgcccg agccgagcgc
     4561 ccgctgccga tggaggcg
//
LOCUS       DBNGOH010000543         2375 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_629241, whole genome shotgun sequence.
ACCESSION   DBNGOH010000543 DBNGOH010000000
VERSION     DBNGOH010000543.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2375)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2375)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2375
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_629241"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..402
                     /locus_tag="AC159P_10560"
     CDS             <1..402
                     /locus_tag="AC159P_10560"
                     /inference="COORDINATES: protein motif:HMM:NF024934.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HIR3695932.1"
                     /translation="ALRMKTEDARDAFRRHEQFQRALLRHMRALLVMTAQTAACNALH
                     TIEERLARWLLLCWQRSGSAVLPLTHEMLSNMLGVRRSGVTVAAGILQRAGLIRYSRK
                     DIVILDREGLEAASCECFRSMSANVPRDDAG"
     gene            595..1029
                     /locus_tag="AC159P_10565"
     CDS             595..1029
                     /locus_tag="AC159P_10565"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3695933.1"
                     /translation="MNPSTMHRRVPRAETAPALSPSTPPPLVLVADDDDDIRLMMKTL
                     LEMKGYRTAMAANGQEAVETAERERPDLILLDLQLPRLNGFAVTRFVRQHDELRCVPI
                     VVVSGHDPARHLNLALAAGCNAYIQKPIDFDQLDDLLVGLLQ"
     gene            complement(1154..2134)
                     /locus_tag="AC159P_10570"
     CDS             complement(1154..2134)
                     /locus_tag="AC159P_10570"
                     /inference="COORDINATES: protein motif:HMM:NF017085.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HIR3695934.1"
                     /translation="MAKAREIAALECDGDGVEGIRRVLLVRLDEVCEFREQALDFSDI
                     KGVHAMRVASRRLRSASRDFAPYLRKGRLDGAVEELRELADLLGGVRDLDVELSALEK
                     LSDEVPPGLAEGVREIVGEREARRERARARLAEDMTAGAVEDLRRKFARAVEDASRAR
                     RRSRKNDDGDATDGPSFRAVGREVSLRLWDELSARSRSLRRPLKSGPLHRMRISAKRL
                     RYALELFAQSHGETLKGFAEEIAEMQGSLGNLHDCDVWVETFGDQLREYADAADSSGD
                     ADHARRRDAAVWLLGHFAEKRTRHYRDALDRWHEWQRSDFAARLAACLEE"
BASE COUNT          386 a          890 c          736 g          363 t
ORIGIN      
        1 gcgctgcgga tgaagaccga ggacgcgcgc gacgccttcc gccgccacga gcagttccag
       61 cgtgccctcc tgcgccacat gcgcgcgctg ctcgtgatga cggcgcagac cgcggcctgc
      121 aacgcgctcc acaccatcga ggagcggctg gcgcgctggc tgctgctgtg ctggcagcgc
      181 tccggctccg ccgtgctgcc gctgacgcac gagatgctct cgaacatgct cggggtgcgc
      241 cgctccggcg tgacggtcgc cgcgggcatc ctgcaaaggg ccgggctgat ccgctacagc
      301 cgcaaggaca tcgtcatcct cgaccgcgag gggctggagg cggcctcctg cgagtgcttc
      361 cggagcatga gcgccaacgt gccgcgcgac gacgccgggt gaatttattc aggcaactgt
      421 ccgtatcagg acagactccg ccgacggcgc ggcatacact tgcgcccacc agaacaaggg
      481 ggaaagggcg aggggcggaa catcctgcaa cgaatgtccg cccttcggca caccacaccg
      541 gccccgacac cgtctacaca tcccggaagc cgacccctcg gctgagagaa cagcatgaac
      601 ccctcgacca tgcatcgccg cgtcccccgc gccgaaacgg cgcccgctct ttccccttcg
      661 acgccgccgc ccctcgtcct cgtcgcggac gacgacgacg acatccgcct gatgatgaag
      721 acgctgctcg agatgaaggg ctaccgcacc gccatggccg ccaacggcca ggaggccgtc
      781 gagaccgccg agcgcgagcg ccccgacctc atcctcctcg acctgcaact gccccgcctc
      841 aacggcttcg ccgtgacccg cttcgtgcgg cagcacgacg agctgcgctg tgtccccatc
      901 gtcgtcgtct ccgggcacga cccggccagg cacctcaacc tcgcgctcgc cgccggctgt
      961 aacgcctaca tccaaaagcc catcgacttc gaccaactag acgacctgct cgtcggcctg
     1021 ctgcaataag caagcaggca gcaggcagta aaagacacag agcgagccgc gaaagaagag
     1081 gcttgagacg ttcagtccca agcctctttg ctcgtgtgat ttttactgcc cgcggcccgc
     1141 tgcctgctgc ctgctactct tccagacacg cggcgaggcg ggcggcgaag tcgctgcgct
     1201 gccactcgtg ccagcggtcg agggcgtcgc ggtagtggcg cgtgcgcttc tcggcgaagt
     1261 ggccgaggag ccagacggcg gcgtcgcggc gtcgcgcgtg gtcggcgtcg ccggaagagt
     1321 ccgcggcgtc cgcgtactcg cgcagttggt cgccgaaggt ctcgacccac acgtcgcagt
     1381 cgtggaggtt gccgagcgaa ccctgcatct cggcgatctc ctcggcgaag cccttcagcg
     1441 tctcgccgtg gctctgggcg aagagttcga gcgcgtaccg cagccgcttc gccgagatgc
     1501 gcatgcggtg gagcggcccc gacttgagcg ggcggcgcag gctccggctc cgcgcgctca
     1561 gctcgtccca cagccgcaga gagacctcgc gccccaccgc gcggaagctc ggcccgtccg
     1621 tcgcgtcgcc gtcgtcgttc ttccggctcc tcctgcgcgc gcgcgacgcg tcctcgaccg
     1681 cgcgcgcgaa cttccgccgc aagtcctcga ccgcgcccgc tgtcatgtcc tccgcgagcc
     1741 gcgcgcgcgc tcgctcgcgc cgcgcctcgc gctcgcccac gatctcgcgc acgccctcgg
     1801 ccagccccgg cggcacctcg tccgacagct tctccagcgc gctcagctcc acatccagat
     1861 cgcgcacgcc gccgagcagg tcggccaact ccctcaactc ctcgaccgcg ccgtccagac
     1921 gacctttgcg caggtacggc gcgaagtcgc gcgacgccga acgcagccgc cgcgacgcca
     1981 cgcgcatcgc gtgcacgccc ttgatgtccg agaagtcgag cgcctgctcg cggaactcgc
     2041 acacctcgtc gagccgcacg agtagcacgc gcctgatgcc ctcgaccccg tcgccgtcgc
     2101 attcgagcgc ggctatctct ctcgcttttg ccataaggtg cagtgttcag tggtcggtga
     2161 aagaagtggt tggcagtagg cagtcggtcg ccgtcgatgg aagtcgtcgt gcaagtagtc
     2221 agtctaataa actcgaactt gaaacttcaa actcgaaccg gcggcaacgt gtctctcact
     2281 gaccgccggc caccgaccac tggccgctgt tgctgacgct gaactccaag cggcagccga
     2341 agacctgttc gaagaggtcg cgcttctgct cggcg
//
LOCUS       DBNGOH010000544         2483 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3195801, whole genome shotgun sequence.
ACCESSION   DBNGOH010000544 DBNGOH010000000
VERSION     DBNGOH010000544.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2483)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2483)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2483
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3195801"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..574
                     /locus_tag="AC159P_10575"
     CDS             <1..574
                     /locus_tag="AC159P_10575"
                     /inference="COORDINATES: protein motif:HMM:NF012488.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HIR3695935.1"
                     /translation="TVAQTGCDFFVAGTHKWLFGPRGTGLDWAKAATWKQLRPTFPAF
                     DAGPFAAWLRGEAPRGTEAAWISPGGFHSFEYSWALSAAFDFHGLIGRPRVAQRIHSL
                     NEQCKEGLARMRHVKLHTRRGSRLSAGLVCFDVEGMKPQEVVARLLARGIIASTAPYA
                     VPCVRLAPSLLNSPEEIETALREVRALAAV"
     gene            702..1871
                     /locus_tag="AC159P_10580"
     CDS             702..1871
                     /locus_tag="AC159P_10580"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015347986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004130 - cytochrome-c peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c peroxidase"
                     /protein_id="HIR3695936.1"
                     /translation="MILLTAAFALSGRAESEQERSSPARGGDFEGGDAEGGSGHGRDG
                     KRLFERETFGGNGRTCLTCHSPETGTLSPQDAQRRFAEDPRDPLFLHDGSDDGQGNGV
                     SRMLGDATVLVEVPLPPNVSLADDPTARTVTLRRGIPSTLNTPALDRVLMLDGREPDL
                     QTQALGAIRSHAQSTEEPSAGDLRRIAEFERSKDFFSSDALRRFAAGGPAPVLPEGST
                     PSERRGRRFFEDAADPADPKVGSCAACHSGPMLNETNRFLPLPVPPGTRFQNILVSEF
                     NAAGNPIRTFVFTNPDGTQTFVHSPDPGRALVTGDPRQFPFDSLNAFKIPSLWGTSRT
                     APYFHDNSAETLEDVAAHYARFFAVVTTPPGGGVPGILLTDEDRADIVAYLKLLE"
     gene            2047..>2483
                     /locus_tag="AC159P_10585"
     CDS             2047..>2483
                     /locus_tag="AC159P_10585"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012018821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HIR3695937.1"
                     /translation="MQATGGVYARSESVHITPGEMRRAFPLPENVPNFIAHGRRVIRD
                     ILDGADQRLFVVVGPCSIHDPAAALEYARRLKGLADKVSDTLHLVMRVYFEKPRTTTG
                     WKGLINDPHLDGSLRINEGLTTARRLLINIAELGLPAATEALDP"
BASE COUNT          435 a          847 c          841 g          360 t
ORIGIN      
        1 gaccgtcgcg cagacgggct gcgacttctt cgtcgccgga actcacaagt ggctcttcgg
       61 cccgcgcggg acgggcctcg actgggcgaa ggcggccacc tggaaacagt tgcgcccgac
      121 cttcccggcc ttcgatgccg gccccttcgc ggcgtggctg cgaggagagg cgccgcgcgg
      181 gacggaggcg gcgtggatct cgccgggcgg gttccattcc ttcgaatatt cctgggcgtt
      241 gtcggcggcg ttcgacttcc acggtctgat cggtaggccg agagtcgcgc agcgaattca
      301 tagtttgaac gaacaatgca aggaggggct ggcgcggatg cgccacgtca agctccacac
      361 gcggagggga agcaggctgt cggcgggtct ggtctgcttc gatgtcgaag ggatgaaacc
      421 gcaagaggtc gtggcgcggc tgctcgcacg gggcatcatc gcctcgacgg caccctacgc
      481 cgtcccttgc gtaagactgg ccccgagtct gctgaactcg ccggaagaga tcgagacggc
      541 cctccgcgag gtccgcgccc tcgccgcggt ctgagcgcga cctgcgaggc gaacgccgac
      601 gcgccgggca ttcttcggcg cggaacataa ggagctatct gaaaagaaag gtctacgtcg
      661 tgaacagcct gagaaccgcg aaattagtaa tcaccgtagc aatgatcctc ttgaccgcgg
      721 cgttcgccct gtccggtcgc gccgagtccg aacaggagcg gagcagtccg gcccggggcg
      781 gagactttga agggggcgat gccgagggag gctccggcca cgggcgcgac ggcaagcgtc
      841 tgttcgagcg ggagaccttc ggcggcaacg ggcgcacctg cctgacctgc cacagccccg
      901 agaccggaac cctctcgccc caggacgcgc agcggcgctt cgccgaggac ccgcgcgacc
      961 cgctcttcct ccacgacggc agcgacgacg gccagggcaa cggcgtctcg cgcatgctcg
     1021 gcgacgccac cgtcctcgtc gaagtgcccc tgccgcccaa cgtgtccctc gccgacgacc
     1081 cgaccgcgcg cacggtgacg ctcaggcgcg gcatcccgtc cacgctcaac accccggccc
     1141 tcgaccgggt cctcatgctc gacgggcgcg agccagacct ccagacgcag gcgctcggcg
     1201 ccatccgcag ccacgcgcag agcacggagg agccgagcgc gggagacctc cggcgaatcg
     1261 ccgagttcga gcggtcgaag gacttcttca gctcggacgc gctgcggcgt ttcgccgccg
     1321 gcggccccgc gcccgtgctg cccgagggga gcacgccctc cgagcggcgc gggcggcgct
     1381 tcttcgagga cgcggcggac cccgcagacc cgaaggtcgg ctcctgcgcc gcctgccaca
     1441 gcggcccgat gctgaacgag acgaaccgct tcctgccgct gccggtcccg cccgggacgc
     1501 gcttccaaaa catcctcgtc tcggaattca acgctgccgg aaacccgatc cgcacgttcg
     1561 tcttcacgaa tcccgacggc acacagacgt tcgtgcattc gcccgacccg ggccgcgcgc
     1621 tggtgacggg cgacccgcgg cagttcccat tcgacagcct gaacgcattc aaaatcccgt
     1681 cgctgtgggg aacgagccgg acggccccgt attttcacga caactcggcg gagacgctcg
     1741 aagatgtcgc ggcgcactac gcgcgtttct tcgcggtggt cacgacgccg ccgggcggcg
     1801 gcgtcccggg catcctcctg actgatgagg atcgggccga catcgtggct tacctgaagc
     1861 tcctcgaatg aagaaggagc gcgcgcgaag tggccgccga accgcggcca gatgccgagg
     1921 cggcgcggag ggtagggcgc acactcaaga cacgaaggaa gactcaacat gtcccaacaa
     1981 atggtcgtca ccgacacgcg ggccgggcgc gcttggagca ggcgcgcgcg ggggggccgg
     2041 cgcccgatgc aagccaccgg cggcgtatac gccaggtccg agagtgttca catcacgccg
     2101 ggggagatga ggcgggcgtt tccgctaccg gagaacgtcc cgaatttcat cgcacacggg
     2161 agacgggtca tcagggacat tcttgatggc gccgaccaac ggttattcgt cgtggtggga
     2221 ccctgttcaa tccatgaccc cgcggcggcg ctcgagtacg cgcgccggct gaaggggttg
     2281 gccgacaagg tcagcgacac cctccatctg gtgatgcgcg tgtatttcga gaagccgcgc
     2341 acgacgaccg gctggaaggg gttgattaac gacccccatt tagacggcag cttacgcatc
     2401 aacgaaggtt tgacgaccgc gcggcggctc ctgatcaaca tcgcggagtt agggctgccg
     2461 gcggcgaccg aagccttaga ccc
//
LOCUS       DBNGOH010000545         6928 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3930509, whole genome shotgun sequence.
ACCESSION   DBNGOH010000545 DBNGOH010000000
VERSION     DBNGOH010000545.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6928)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6928)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6928
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3930509"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1025)
                     /locus_tag="AC159P_10590"
     CDS             complement(<1..1025)
                     /locus_tag="AC159P_10590"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HIR3695938.1"
                     /translation="MNFFEFVQQNWRDILALTGEHLFLVLVSTALAVAVGLPVGLLLT
                     RREALKRPVLGVANVLQTVPSLALFAFLIPIKWVGIGWRPAIIALFLYALLPIIRNTV
                     AGVEGVDRSVRETAVAMGMTGGQILRQVELPLAAPVVLAGVRVAAVISVGVATIAAFI
                     GAGGLGVYIQRGLRQYDQNLLLAGAVPAALLALAADASIGLLESRLDARRRGALAPRK
                     WGAISRRLAWAALALVVVIGAASAAWRGTRKADVVVGSKDFTESVILGEMLAQTLEAE
                     GLKVTRRFELGGTIAHDSLVAGEVDVYPEYTGTSLIDILKHPPATDARAVYERVKADY
                     ASRFDVEV"
     gene            complement(1098..1904)
                     /locus_tag="AC159P_10595"
     CDS             complement(1098..1904)
                     /locus_tag="AC159P_10595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012409202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR3695939.1"
                     /translation="MSRPETQNTTAAERDDPVVRFSRATYAVPAAPAPIIDDLDLDIR
                     RGETLVLLGESGCGKTTTLRLVNRLLVPTAGAVSVEGKDTRGWDAIELRRRTGYVIQE
                     AGLFPHFTVERNVGLVPALEGWDAARIGERVRELLQLVGLEPEKFAARFPAELSGGQR
                     QRVGVARALAADPPLLLMDEPFGALDPLTRAGLQREFAALQKRLAKTVVFVTHDVREA
                     LLLGTRVGLMKAGRLVLLETPEGFMRSDEPLARAYRETLRMPEGVSAALT"
     gene            complement(1901..3301)
                     /locus_tag="AC159P_10600"
     CDS             complement(1901..3301)
                     /locus_tag="AC159P_10600"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF016032.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HIR3695940.1"
                     /translation="MSHDDTDGAGDVRRVETARESFPLLTEPVSKESAPLFAENLRRG
                     AKPPAGWTCGVEFELFGYDRRRDFRRLDPAQVQRVLAGFAPNSGDLVYEGGTLVEVDA
                     GQMNRLTIEPGGQIEFSGAPQRSLKDVEREVRRFLARLHEIAEGHGFAFVAAGFDPVR
                     NLAEQQWFPKMRYEVMRPYLARRGARAWDMMARTCAVQCNLDYGDEADLAKKFLVGNR
                     LAPVVTAIFANSPFEDGRPSGYKSTRYAAWLETDPDRSGIPPCALADDFTPEAFVAYA
                     LEVPMLFAQRDGLYLDAPTGMRFGEFLEHGAGTVRPVFGDWADHLTTIFTDARLKQHI
                     ELRGADCNDLGMTLALQALWKGLFYDRAALDEAHRLAPRLGREDSVLLRERAARDGLG
                     ARHAGVDVSGLAREVVTLAAEGLGRTAPDEQSYLDILLERVVAEGVSPADILLRNYEG
                     RWHHSIDRALEHLRIA"
     gene            complement(3464..4507)
                     /gene="egtD"
                     /locus_tag="AC159P_10605"
     CDS             complement(3464..4507)
                     /gene="egtD"
                     /locus_tag="AC159P_10605"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015877793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052706 - L-histidine
                     N(alpha)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HIR3695941.1"
                     /translation="MKAARTADKESGGRLRVHALARGEAGEGLAGDVRRGLTATRKFL
                     LPKYFYDELGSRLFDAICLLPEYYLTRAEDEIFARHAADIVEAAAGGDGDREGPRRLS
                     LLELGSGSATKTRRIIEALVGRQGRLVYVPVDISPAALEESARLLLQSYPALRVEAYA
                     GDYAAALPRLREHVEEDARALVLFLGSNIGNYDPEEARSLLRALRLALRAGDRVLVGA
                     DLKKDKATLEAAYDDALGVTAAFNLNLLARINRELGADFDPRRFRHVAVYDERAGRVE
                     MHLESTRAQTVRVGALDLAVTFAEGERVHTENSYKYDLRELLSLAHATGFRSTNTWLD
                     EAERFSSNLFVAV"
     gene            4706..6031
                     /locus_tag="AC159P_10610"
     CDS             4706..6031
                     /locus_tag="AC159P_10610"
                     /inference="COORDINATES: protein motif:HMM:NF015722.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HIR3695942.1"
                     /translation="MKEQAEEIAREMAEVRAATIELFDTAREEDLRESPGFGFRPVVW
                     HLAHIGVFEAYWLLQKLRGEPPPDEHYERVFDPIQTPREESKNLPSRREMEGYLARVR
                     ERSLRSLEEADFDSPDPLLRKGYVFRLVLEHERQHQETLAYLLQLLDPSRKTRPAARG
                     LVHTSDAFPPTAETSPNTLARDMLDVPAGPFEMGAVWDSFAYDNEYPAHTVSIPAFKI
                     ARRPVTNEEFAEFVAERGYERREFWSEEGRQHRERENWTCPLYWRCEGAGVWHARTMF
                     DSGALKLDHPVAGISWHEAEAYARFRGKRLPTEAEWEKAASWDEGAGRKRRYAWGDRE
                     PSDALANYGARRWGTTPVGSFPAGASAYGCLDMTGNVWEWTSTPFDAFHGFRPFPYPE
                     YSETWFDGDHRVLKGGSWATSAPVLRTSFRNFFRRHFRIAFAGLRLAED"
     gene            6053..6856
                     /locus_tag="AC159P_10615"
     CDS             6053..6856
                     /locus_tag="AC159P_10615"
                     /EC_number="3.5.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF014670.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HIR3695943.1"
                     /translation="MRPLLLLLLLTVVLPVAATGQTKSKGILLEDLTWTEAEKVLTEK
                     TVVVSPLGAAAKEHGPHLKLNNDWLISEYLKRRVLEGSEVVVAPTVNYHFYPPFLEYP
                     GSTSLRLETARDLVIDICRSLARYGPRRFYVLNTGVSTLRALQPAADALAKEGLALRY
                     TDLLKITGPVEKAVGQQEGGTHADEIETSMMLYMAPATVDMKKAAKDYHPSRERGLTR
                     NPQGKGVYSPTGIYGDATLATRAKGEKITKALVAGILTEIEELRRSPLP"
BASE COUNT         1109 a         2429 c         2317 g         1073 t
ORIGIN      
        1 acctccacgt cgaagcgcga ggcgtagtcc gccttgacgc gttcgtagac ggcgcgcgca
       61 tcggtcgccg gcgggtgctt gaggatgtct atcagcgacg tgcccgtgta ctcggggtac
      121 acgtcaacct cgcccgcgac gagcgagtcg tgcgcgatgg tgccgcccag ctcgaagcgc
      181 cgcgtcacct tcagcccttc ggcttcgagc gtctgcgcca gcatctcgcc gaggatgacc
      241 gactccgtga agtccttcga gccgacgacc acgtccgcct tgcgcgtgcc ccgccacgcg
      301 gcggacgccg cgccgatgac gacgacgagc gcgagcgccg cccatgccaa ccgtctcgat
      361 atcgcgcccc acttccgcgg cgcgagcgcg ccgcgccgcc gcgcgtcgag gcgcgattcg
      421 agcaggccga tggaggcgtc cgccgcgagc gcgagcagcg ccgccggcac cgcgcccgcg
      481 agcagaaggt tctggtcgta ctggcgcagg ccccgctgga tgtacacgcc gaggccgccc
      541 gcgccgatga acgccgcgat ggtcgccacg cccaccgaga tgaccgccgc cacgcgcacg
      601 cccgcgagga cgaccggcgc cgccagcggc agctccacct gtctgagaat ttgcccgccc
      661 gtcatcccca tcgccaccgc cgtctcgcgc acgctgcggt caaccccttc gacgcccgcg
      721 accgtgttgc ggatgatggg cagcagcgcg tagaggaaga gcgcgatgat ggcgggccgc
      781 cacccgatgc cgacccattt gatcgggatg aggaaggcga agagcgcgag tgaaggcacg
      841 gtctgcaaca cgttggcgac gccgagcacg ggccgcttca gcgcctcgcg gcgcgtcagc
      901 agcagcccga cgggcagccc gacggcgacg gccagcgccg tcgagacgag caccagaaac
      961 aggtgctcgc ccgtcaacgc gaggatgtcg cgccagttct gctggacgaa ctcgaagaag
     1021 ttcaacgcct gcgctccgtg gtcatctctc ctcgatgaag tcgagggtcg cgggagtcgg
     1081 acactcgaca acggctctca agtcaacgcc gccgacacgc cttcgggcat gcgcagcgtc
     1141 tcgcggtagg cgcgcgccag cggctcgtcc gagcgcatga agccctccgg cgtttcgagc
     1201 aggacgaggc ggcccgcctt catcaagccg acgcgcgtcc ctaacagcag cgcttcgcgc
     1261 acgtcgtgcg tgacgaagac gaccgtcttg gccagacgct tttgcagcgc cgcgaactcg
     1321 cgctgaaggc cggcgcgcgt caaggggtcg agcgcgccga acggctcgtc catcaacagg
     1381 agcggcgggt ctgcggcgag cgcgcgcgcg acgcccacgc gctgccgctg cccgcccgac
     1441 agctctgcgg ggaaacgcgc ggcgaacttc tccggctcaa gcccgacgag ctgtaacaac
     1501 tcgcgcacgc gctcgccgat gcgcgccgcg tcccagcctt cgagcgcggg caccagcccg
     1561 acgttgcgct cgaccgtgaa gtgcgggaac agcccggcct cctgtatgac gtagcccgtc
     1621 cgccgcctca gctctatcgc gtcccacccg cgcgtgtctt tgccttcgac cgagaccgcg
     1681 cccgccgtcg gcacgagcag gcggttgacg agccgcagcg tcgtcgtctt cccgcacccg
     1741 gactcgccga gcagcaccag cgtctcgccg cgacgaatgt cgaggtcgag gtcgtcgatg
     1801 atcggcgccg gagccgccgg cacggcgtag gtcgcgcggc tgaagcggac gaccgggtcg
     1861 tctcgttcgg cggcggtcgt gttctgcgtc tcggggcggc tcatgcgatg cgcaggtgtt
     1921 cgagggcgcg gtcgatggaa tggtgccaac ggccctcgta gttgcgtaag agaatgtcgg
     1981 cgggcgagac gccttcggcg acgacgcgct caaggaggat gtcgaggtaa gactgctcgt
     2041 ccggcgccgt cctcccgaga ccttccgccg cgagcgtgac cacttcccgc gccagccccg
     2101 acacgtccac gcccgcgtgg cgcgcgccga ggccgtcgcg cgccgcccgc tcgcgcagga
     2161 ggacggaatc ttcgcggccc aggcgcggcg cgaggcggtg tgcttcgtcg agcgcggccc
     2221 ggtcgtagaa gagtcccttc cagagcgctt gcagcgcgag cgtcatcccg aggtcgttgc
     2281 agtccgcgcc gcgcagctcg atgtgctgtt tgaggcgcgc gtccgtgaag atggtcgtca
     2341 ggtggtcggc ccagtcgccg aagacggggc ggacggtgcc tgcgccgtgc tcaaggaact
     2401 cgccgaagcg catccccgtc ggcgcgtcga ggtagaggcc gtcgcgctgc gcgaagagca
     2461 tcggcacttc caatgcgtag gcgacgaagg cttcgggcgt gaagtcgtcc gcgagcgcgc
     2521 agggcgggat gccggagcgg tcggggtcgg tctcgagcca cgcggcgtag cgcgtggact
     2581 tgtagcccga agggcgcccg tcctcgaagg gcgagttggc gaagatcgcc gtcacgacgg
     2641 gcgcgaggcg gttgccgacc agaaactttt tggcgaggtc tgcttcgtcg ccgtagtcga
     2701 ggttgcactg cacggcgcac gtgcgcgcca tcatgtccca cgcgcgcgcg ccgcgccggg
     2761 cgaggtaggg gcgcatcact tcgtagcgca tcttcgggaa ccactgctgc tccgcgaggt
     2821 tgcgcaccgg gtcgaagccc gcggcgacga aggcgaagcc gtgcccctcg gcgatctcgt
     2881 gcaggcgcgc gaggaagcgc cgcacctcgc gctcgacatc cttcaggctg cgctgcggcg
     2941 cccccgaaaa ctcgatctgc ccgcccggct cgatggtcag gcggttcatc tgccccgcgt
     3001 ccacctcgac gagcgtgccg ccctcgtaga ccaggtcgcc cgagttcggc gcgaagcccg
     3061 ccagcacgcg ctgcacctgc gccgggtcga gccgccggaa gtcgcgccgg cggtcgtagc
     3121 cgaacagctc gaactcgacg ccgcacgtcc accccgcggg cggcttcgcc ccgcgccgca
     3181 aattctccgc gaagagcggc gccgactcct tcgacaccgg ctcggtcagc agcgggaagc
     3241 tctcgcgcgc cgtctcgacg cgtcgcacgt cgccggcacc gtctgtgtcg tcgtgagaca
     3301 taagaaagaa gtgatgaatg cagagtgcgg aatgatgaat ggaagaccgt tatcagttat
     3361 cggttttcgg ttttcggttg tcagttaaag acattcgctc ttgctgaaaa ccgaaaactg
     3421 atgactgttt catcattctg cactctgcat tcatcatttt atttcaaacc gcgacgaaga
     3481 ggttgctgct gaagcgctcg gcctcgtcga gccacgtgtt ggtgctgcgg aagccggtcg
     3541 cgtgggcgag gctcaagagt tcgcggaggt cgtacttgta agagttctcc gtgtgcacgc
     3601 gctcgccctc cgcgaaggtg acggcgaggt cgagggcgcc gacgcgcacc gtctgcgcgc
     3661 gcgtgctttc gaggtgcatc tcgacgcggc ccgcgcgttc gtcgtagacg gcgacgtggc
     3721 ggaagcggcg cgggtcgaag tccgcgccga gttcgcggtt gatgcgcgcg aggaggttga
     3781 ggttgaaggc ggcggtgacg ccgagcgcgt cgtcgtaggc ggcttcgagc gtcgccttat
     3841 ccttcttcaa gtcggcgccg acgagcacgc ggtcgcccgc gcgcaaggcg aggcgcaaag
     3901 ctcgcagcag cgagcgcgcc tcctcggggt cgtagttgcc gatgttcgag ccgaggaaga
     3961 ggacgagggc gcgcgcgtcc tcttcgacgt gctcgcgcag acgcgggagc gccgccgcgt
     4021 agtcgcccgc gtaagcctcg acgcgcaaag ccgggtagga ttgcaggagc aggcgcgcgc
     4081 tctcttcgag cgcggcgggc gagatgtcca cggggacgta aacgagccgg ccttgtcttc
     4141 cgacgagcgc ttcgatgatg cggcgcgtct tggtcgcgct gcccgacccg agttcgagca
     4201 gcgagaggcg gcgcgggcct tcgcggtcgc cgtcgccgcc cgccgcggcc tcgacgatgt
     4261 cggcggcgtg gcgcgcgaag atttcgtcct cggcgcgcgt caggtagtat tcgggcagca
     4321 ggcagatggc gtcgaagagg cgcgagccga gttcgtcgta gaagtatttc ggcaggagga
     4381 atttccgcgt ggcggtcagc ccgcgccgca cgtctccggc cagcccctcg cccgcctcgc
     4441 cgcgcgcgag ggcgtggacg cgcagccgcc cgcccgactc tttgtccgca gttcgcgccg
     4501 ccttcacgtc tctttcgtca cctccgattt cttcgacccc gcgacggcga tgccgctccc
     4561 tgtgtacacg tcgaagcgcc ccgactcgcc gcgtccgcct ttcgttctcc gagcttgcca
     4621 tgatagcgca tcgtgcctca ctatccacgt gcggcggcgt cgctttttcg tcacgcgcgc
     4681 cgccgaccgg agcggagtcc gtttgatgaa agaacaggcc gaagagatcg cgcgcgagat
     4741 ggcggaggtg cgcgccgcga ccatcgagct tttcgacacg gcgcgcgaag aggacttgcg
     4801 cgaaagcccc ggcttcggct tccggcccgt cgtctggcac ctcgcgcaca tcggcgtctt
     4861 cgaggcttac tggctgctgc aaaaactgcg cggcgagcct ccgcccgacg aacactacga
     4921 gcgcgtcttc gaccccatcc aaacgccgcg cgaagagtcc aagaaccttc cctcgcggcg
     4981 cgagatggaa ggctatctcg cgcgcgtgcg cgagcgctcg ctccgcagtc tcgaagaggc
     5041 ggacttcgac tcgccggacc ctctgctccg caagggctac gtcttccgcc tcgtcctcga
     5101 acacgagcgg cagcatcagg agacgctcgc ctacctcctc caactgctcg acccctcgcg
     5161 gaagacgcgg cccgccgcgc gcggactcgt ccacacttcc gacgcgtttc cgcccaccgc
     5221 cgaaacctct ccgaacactc tcgcgcgcga catgctcgac gtgcccgcag ggcctttcga
     5281 gatgggcgcc gtgtgggact cgttcgccta cgacaacgaa tatcccgcgc acaccgtttc
     5341 tattcccgcc ttcaagatcg cgcgccggcc cgtgacgaac gaggagttcg cggagttcgt
     5401 cgccgagcgc ggctacgagc ggcgcgagtt ctggagcgaa gagggccggc aacaccgcga
     5461 gcgcgagaac tggacgtgcc cgctctactg gcgatgcgaa ggcgcgggcg tctggcacgc
     5521 gcggacgatg ttcgacagcg gcgctctcaa gctcgaccac ccggtcgcgg gcatcagttg
     5581 gcacgaggcc gaggcgtacg cgcgctttcg cggcaagcgc ctgccgaccg aggccgagtg
     5641 ggagaaggcc gcctcgtggg atgaaggcgc aggccgcaaa cgccgctacg cctggggcga
     5701 ccgggagcct tcggacgcgc tcgcgaacta cggcgcgcgc cgctggggca cgacgcccgt
     5761 cggtagtttc cccgcgggcg cctctgccta cgggtgcctc gacatgaccg gcaacgtctg
     5821 ggagtggaca tccacgccct tcgacgcctt ccacggcttc cgccccttcc cctaccccga
     5881 atactccgag acgtggttcg acggggatca ccgcgtcctc aaaggcggct cgtgggccac
     5941 cagcgccccc gtcctgcgca cctcgttccg caacttcttc cgccgccact tccgcatcgc
     6001 cttcgccggc ctgcgcctcg cggaggactg acgcgatcag ggtgaatgaa cgatgagacc
     6061 gctgcttctg ctcctcctgt taaccgtcgt gcttcccgtc gcggccacgg gccagacgaa
     6121 aagcaagggc attctgctgg aagacctcac gtggactgag gcggaaaagg tgctgaccga
     6181 gaagacggtc gtggtcagcc cgctcggcgc ggcggcgaaa gaacacggcc cccacctcaa
     6241 gctcaataac gactggctca tctccgagta tctcaagcgg cgcgtgctcg aaggctcgga
     6301 ggtcgtggtc gcgccgaccg tcaactacca tttttacccc ccctttctgg aataccccgg
     6361 ctccacctcg ctgcgtctgg agacggcgcg cgacctcgtc atcgacatct gccggagcct
     6421 cgcccgctac ggcccgcgcc gcttctacgt cctcaacaca ggcgtctcca ccctgcgtgc
     6481 tctgcaaccc gccgccgacg ctctggcgaa agagggtctg gcgctccgct acaccgacct
     6541 gctgaaaatc accgggccgg tcgagaaggc cgtcggccag caggagggcg gcacgcacgc
     6601 cgacgaaatc gagacttcga tgatgctcta catggcgccc gcgaccgtcg atatgaagaa
     6661 ggcggcgaag gactatcacc cgagccggga gcgcgggctg acccgcaacc cgcaggggaa
     6721 aggggtctat tcgccgaccg ggatttacgg ggacgccaca ctcgccaccc gtgcgaaggg
     6781 cgaaaagata acgaaagctc tggtggccgg cattctcacg gagatcgaag aactccgccg
     6841 gagtcctttg ccgtagggcg ccacacagtc ttcgcgctaa cgattttccc gccccgcccg
     6901 tctaaatctc cgtaaacatt gcgcgcgg
//
LOCUS       DBNGOH010000546         1500 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3562438, whole genome shotgun sequence.
ACCESSION   DBNGOH010000546 DBNGOH010000000
VERSION     DBNGOH010000546.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1500)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1500)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1500
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3562438"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1154)
                     /locus_tag="AC159P_10620"
     CDS             complement(<1..1154)
                     /locus_tag="AC159P_10620"
                     /inference="COORDINATES: protein motif:HMM:NF051698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR3695944.1"
                     /translation="MKVPQRIVGRAFGFVLLFLLAVSNVNAQTTGTLRGQIKDELGAV
                     IVGATVSAVDANGLAKNTTSDADGNFALGGLVPGNYIVRVVAPGFAFYENAEVVVAAG
                     RASAPLVITLGVSLEKEEVTVAAEGGISTDSTSNADAIVLRGKDLEALPDDPDDLAAA
                     LSALAGPSAGPNGGQIYIDGFTGGRLPPKDAIREVRVNQSPMNAENDRPGFGRIDILT
                     RPGMDRLRGSANTSFADEAFNSRNPFSPTRADYQSRIYGFSLSGPIVAKKSSFFVDFQ
                     RREEDDNDVIAARVLDPLTEADTGFNLAVLTPRRFTTFSPRFDYAFNQNHTLIARYSY
                     TRSSNLGGIGAFNLLSRAVDFASTDHNVQLTETAVISPTMINETRFQFTDS"
BASE COUNT          219 a          444 c          579 g          258 t
ORIGIN      
        1 gagtccgtga actggaagcg cgtctcgttg atcatcgtcg ggctgatgac ggcggtctcc
       61 gtgagctgga cgttgtggtc ggtgctcgcg aagtccaccg cgcgcgagag caggttgaag
      121 gcgccgatgc cgccgaggtt gctcgaacgc gtgtaggagt agcgcgcgat gagcgtgtgg
      181 ttctggttga aggcgtagtc gaagcgcggg ctgaaggtgg tgaagcggcg gggcgtgagc
      241 accgccagat tgaagcccgt gtcggcctcg gtcaaggggt cgagcacgcg cgcggcgatg
      301 acgtcgttgt cgtcctcttc gcggcgctgg aagtcaacga agaaggagga cttcttggcg
      361 acgatggggc cgctcaggct gaagccgtag atgcgcgact ggtagtctgc gcgcgtgggc
      421 gagaaggggt tgcgcgagtt gaaagcctcg tcggcgaagg aggtgttggc gctgccgcgc
      481 aggcgatcca tgccggggcg cgtgaggatg tcgatgcgcc cgaagccggg gcggtcgttc
      541 tcggcgttca tcgggctttg gttgacgcgc acctcgcgga tggcgtcctt cggcggcagc
      601 cgcccgcccg tgaagccgtc gatgtaaatc tgcccgccgt tggggcctgc ggaggggccg
      661 gccagcgccg acagggcggc ggccagatcg tccgggtcgt ccggtagcgc ttcgaggtct
      721 ttgccgcgca agacgatggc gtcggcgttg ctcgtcgagt cggtgctgat gccgccttcg
      781 gcggcgaccg tcacctcttc tttttcgagc gagacgccga gcgtgatgac gagcggcgcc
      841 gaggcgcgcc cggcggcgac cacgacttcc gcgttctcgt agaaggcgaa gccgggcgcg
      901 acgacgcgca cgatgtagtt gccgggcacg aggccgccga gcgcgaagtt gccgtcggcg
      961 tcgctggtcg tgttcttggc gaggccgttc gcgtccacgg cgctgacggt cgcgccgacg
     1021 atcacggcgc cgagttcgtc tttgatctgg ccgcgcaggg tgccggtcgt ttgtgcgttg
     1081 acgttcgaga cggcgagcag gaagaggagg acgaagccga aggcccgccc gacgattctc
     1141 tggggtactt tcatgatctt taccttcagg aggtctgggg ttaaaacctg ccgcgcgagg
     1201 ggcgcgtccg aacagcgaac cgtgcgacga gagaaactgt gcgagggtgc tgcggtatta
     1261 actgacggtc gatggttgcc cccggcgcgc gatcgctacg gttagtgacc gcgcgccgcg
     1321 gtgtgatgca agtttgttac ggctggccga tgccgaagcc gaagttggcg ccgccgccgc
     1381 cgagtgcgcc gccgccaccg ccgccgccgc ccggcccgcc gccggggccg cccgcgcgcc
     1441 gcgtcatcat ggcgaccaga ggctcgacgc ccgaaaggag ctggatggcc gtgacgcgcg
//
LOCUS       DBNGOH010000547         3894 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3196070, whole genome shotgun sequence.
ACCESSION   DBNGOH010000547 DBNGOH010000000
VERSION     DBNGOH010000547.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3894)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3894)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3894
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3196070"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..650)
                     /locus_tag="AC159P_10625"
     CDS             complement(<1..650)
                     /locus_tag="AC159P_10625"
                     /inference="COORDINATES: protein motif:HMM:NF048579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695945.1"
                     /translation="MFSDQFRRGETERPRFSGVRSSKLISGLSDAAWKVFQSINTRLP
                     EGEAVRPKWAPGPLLKSYERTAPPLGFPRETDSLCPTCVKEVRTAVIKGDVSLETLMH
                     AHPGEIKAQIVEEGGKVYMTKTCPKHGEFKDVMATDARFLERIESLFFGRDFRAADDK
                     HVHHHGTSNIKFGRGAVLTVDLTNRCNMMCNPCFMDANQVGYVHEPTFQDTKDILDR"
     gene            1020..1856
                     /gene="lpxA"
                     /locus_tag="AC159P_10630"
     CDS             1020..1856
                     /gene="lpxA"
                     /locus_tag="AC159P_10630"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HIR3695946.1"
                     /translation="MPRLRSLRMDIHPTAVVSPRARLARGVRVGPYAVVEDEVSVGEG
                     CEIGAHAVVKRFTALGRRNRVFEHATLGGEPQDVKFGGEPSALVVGDDNLIREYVTIH
                     RASGEGDVTRVGSRNFLMVGVHVAHNCEVGDDNIFANGAALAGHIRVEDHAFLSNNVG
                     CHQFVRIGRYAMVGGKSKIVQDVLPFFTTDGNPPRARGLNAVGLRRAGFSAQSRGALK
                     RAYRLLFRDALPLEEALMELEAMGDEHVLHLARFIRGSRRGFTRAARGAGAAEELESA
                     AD"
     gene            1922..2299
                     /locus_tag="AC159P_10635"
     CDS             1922..2299
                     /locus_tag="AC159P_10635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695947.1"
                     /translation="MSGYGPEKPRRKKLKIVLVVLVFVVALAGVGVVRGWRAFVRFGV
                     SGDLTKYQASISASDLDPQTKRRLVGRIDLIRESARDKSIGFFRWLSYDESFKAIFED
                     QRVTPDEALVLERELSRLEREFE"
     gene            2323..3402
                     /gene="lpxD"
                     /locus_tag="AC159P_10640"
     CDS             2323..3402
                     /gene="lpxD"
                     /locus_tag="AC159P_10640"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HIR3695948.1"
                     /translation="MNDETRRKTAAELAELVGGRVVGDGGVVLKGVAAVESARAGDVV
                     FAEDAKTVESARGCGASCVIVPEGARIEGAPCLIEVARPKLAFALVARELRPAKRREA
                     TVHPTASVAAGATLGEGVHVGACAVVGEGARVGERTQLLAGVVVGEGATVGRECVLHP
                     GVVLYENVTIGDRVVLHAGVVIGADGFGYVRDAEGRYHKFPQVGTVVIEDDVEIGAHT
                     CVDRGALGETRVGRGTKIDNLVQVAHNVQIGERVVIAAQTGISGSTVIESDAVIGGQV
                     GMGDHARVQSGAVIGSQAGVLPGKIVRAGVWWGTPVQPLEEYKRLNAMWGRLPRMREE
                     IKELRAQLKELVARLDGRGGDEGKR"
     gene            complement(3493..>3894)
                     /locus_tag="AC159P_10645"
     CDS             complement(3493..>3894)
                     /locus_tag="AC159P_10645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase DCC family
                     protein"
                     /protein_id="HIR3695949.1"
                     /translation="LLYDGVCGFCNETVRTLLRLDPRGTLRFAALQSDYGQRVVARHP
                     ALKGVDSVVFVEPATDDAAERVHVRSDAALRVAAYLGGPWKIFLLARLLPRRLRDFLY
                     DTFARNRYKIFGKYDTCLLPAPEVRSRFLDV"
BASE COUNT          633 a         1132 c         1505 g          624 t
ORIGIN      
        1 cggtctaaga tgtctttcgt atcctgaaac gtgggttcgt gtacgtaacc gacctgattc
       61 gcgtccatga aacaagggtt gcacatcatg ttgcagcggt tcgtcaggtc aaccgtgagg
      121 acggcgccgc gaccgaactt gatgttggag gtgccgtgat ggtggacgtg cttgtcgtcg
      181 gcggcgcgga agtcgcgtcc gaagaagagc gactcgatgc gttcgaggaa gcgcgcgtcc
      241 gtcgccatca cgtccttgaa ctcgccgtgc ttcgggcagg tcttcgtcat gtagaccttg
      301 ccgccctctt cgacgatctg cgccttgatc tcgccggggt gcgcgtgcat cagcgtttcg
      361 agcgacacgt cgcccttgat gacggcggtg cggacctcct tgacgcaggt cgggcacaag
      421 ctatcggttt cgcgcgggaa gccgagcggc ggggcggtgc gctcgtagga cttgaggagc
      481 ggccccggcg cccacttggg ccggacggcc tcgccctccg gcaggcgcgt gttgatggac
      541 tggaacacct tccacgcggc gtccgagagg cccgaaatca acttcgagct gcggacgccg
      601 ctgaagcgcg ggcgttctgt ctcgccccgg cggaactggt cgctgaacaa tgtctctctc
      661 ctcttagtca gggaacgcgg acgaaccaca gcgtccgaca cttgcctgat gaagaccgaa
      721 aatgaagaag aataaacggg gacggcgtct gtgcttatca acggaggagc gccgcacccg
      781 cgcgtcatga taaagacatc cgctcgtaaa tacaaggcgt tagacgcgct tagcgcgggc
      841 cgggttgcgc cggatgtcgt gcataggcga ccgaaacggg ccgcggacgg ggcgcacttc
      901 ggacgaagaa acggcgcggc gcgaagcctt tgaactcgtc ggcgcggagc cgtgaacgcc
      961 ttccgcgtcc gccctctccg ccttctattc cgttgccggg cggggcgcga ggatgtacga
     1021 tgccacgtct ccggagcctc cgtatggaca tccacccgac agccgtcgtc agcccgcgtg
     1081 cgcgcctcgc gcggggcgtg cgcgtcggcc cgtacgcggt cgtcgaggat gaagtgagcg
     1141 tcggcgaggg ctgcgagatc ggcgcgcacg cggtcgtcaa gcgcttcacc gcgctgggcc
     1201 ggcgcaaccg cgtcttcgag cacgcaacct tgggcggcga gccgcaggac gtgaagttcg
     1261 ggggcgagcc gagcgcgctc gtcgtcggcg acgacaacct gatccgcgag tacgtgacga
     1321 ttcaccgcgc gagcggcgag ggggacgtga cgcgcgtggg gagccggaat tttctgatgg
     1381 tcggcgtcca cgtcgcgcac aactgcgagg tcggcgacga caacatcttc gccaacggcg
     1441 cggcgctcgc gggccacatc cgcgtcgaag accacgcgtt tctgtcgaac aacgtcggct
     1501 gtcaccagtt cgtgcggatc gggcgctacg cgatggtcgg cggcaagtcg aagatcgtgc
     1561 aggacgtgct gcctttcttc acgacggacg gcaacccgcc gcgggcgcgc ggcctgaacg
     1621 cggtgggcct gcgccgcgcg ggcttctcgg cgcagtcgcg cggcgccctg aagcgcgcct
     1681 acaggctcct cttccgcgac gcgctgccgc tcgaagaggc gctgatggag ttggaggcga
     1741 tgggcgacga gcacgtgctg cacctcgcgc gattcatacg cggctcgcgc cgcggcttca
     1801 cgcgcgcggc gcggggcgcg ggcgcggcgg aagagcttga gagtgccgct gactgaaatg
     1861 ttcgcaaggg ccggtggccg aaacgttccg ggtgtaggcc gtcgaggacg ggggatgaat
     1921 catgagcgga tatgggcctg agaagccgcg gcggaagaag ttgaagatcg tccttgtcgt
     1981 gttggtcttc gtggtcgcgc tcgccggggt cggtgtcgtg cgcgggtgga gggcgttcgt
     2041 gcgcttcggc gtgtcgggcg acctgacgaa gtatcaggcg tcgatcagcg cgagcgacct
     2101 cgacccgcag acgaagcgga ggctggtggg gcgcatcgac ctcatccgcg agagcgcgcg
     2161 cgacaagtcc atcggcttct tccgttggct ctcttacgac gagtcgttca aggccatctt
     2221 tgaagatcaa cgcgtcacgc ccgacgaggc gttggtgctc gaaagggagt tgtcgcgtct
     2281 ggagagggag ttcgagtaag gggcgcgcgc gggcgatcga cgatgaacga cgagacgaga
     2341 cggaagacgg cggcggagct ggccgagctg gtcggcgggc gggtcgtggg cgacggcggc
     2401 gtggtcttga agggcgtggc cgcggtcgag tcggcgcgcg cgggcgatgt cgtgttcgcc
     2461 gaggacgcga agacggtcga gagcgcgagg ggctgcgggg cgtcgtgcgt gatcgtgccg
     2521 gagggcgcgc gaatcgaggg tgcgccgtgt ttgatagaag tggcgcgacc gaagctcgcg
     2581 ttcgccctcg tcgcgcgcga gctgcgaccg gcgaagagga gggaagcgac agtccacccg
     2641 acggcgagcg tggcggcggg cgcgacgctc ggcgagggcg tgcacgtcgg cgcgtgcgcg
     2701 gtcgtgggcg agggcgcgcg cgtcggcgag cggacgcagt tactggcggg cgtcgtggtc
     2761 ggcgaagggg cgacagtcgg gcgcgagtgc gtgctccatc cgggcgtggt gctttacgag
     2821 aacgtgacga ttggcgaccg cgtcgtgctg cacgcgggcg tcgtgatcgg ggcggacggg
     2881 ttcgggtacg tgcgcgatgc agaggggcgc taccacaagt ttccgcaggt ggggacggtc
     2941 gtcatcgagg acgacgtgga gatcggcgcg cacacgtgtg ttgaccgcgg cgcgctgggc
     3001 gagacgcgcg tcgggcgcgg gacgaagatc gacaacctcg tgcaggtggc gcacaacgtg
     3061 cagatcggcg agcgcgtcgt gatcgcggcg cagacgggca tctctgggag cacggtgatc
     3121 gagtcggacg cggtcatcgg cgggcaggtc gggatgggcg accacgcgcg cgtgcagtcg
     3181 ggcgcggtca tcggttcgca ggcgggcgtg ctgcccggca agatcgtgcg agcgggcgtg
     3241 tggtggggca cgcccgtgca gccgctcgaa gagtacaagc ggctgaacgc gatgtggggg
     3301 cggctgccgc ggatgcgcga ggagattaaa gagctgcgcg cgcagttgaa ggagttggtg
     3361 gcgcggctcg acgggcgggg cggagacgag gggaaaaggt gaaaaggtga aaaggggaaa
     3421 agggaagatg acaggcagcg tggctctcgt cctccctctt ccccttttcg cctttccccc
     3481 tttttcgctt tctcaaacgt cgaggaagcg cgagcggact tccggggcgg ggaggaggca
     3541 ggtgtcgtac ttgccgaaga ttttgtagcg gttgcgcgcg aaggtgtcgt agaggaagtc
     3601 gcgcagcctt cggggcagca gccgcgcgag gaggaatatc ttccaggggc cgccgaggta
     3661 agcggcgacg cgcagcgccg cgtccgagcg gacgtggaca cgctccgcgg cgtcgtccgt
     3721 cgcaggttcg acgaagacga cggaatcgac gcccttgagc gcggggtgcc tggcgacgac
     3781 gcgctggccg tagtcgcttt gcagcgcggc gaagcgcagc gtgccgcgcg ggtcgagtcg
     3841 cagcagcgtg cgcaccgtct cgttgcagaa gccgcagacg ccgtcgtaga gcaa
//
LOCUS       DBNGOH010000548         2042 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2646229, whole genome shotgun sequence.
ACCESSION   DBNGOH010000548 DBNGOH010000000
VERSION     DBNGOH010000548.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2042)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2042)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2042
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2646229"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..749)
                     /locus_tag="AC159P_10650"
     CDS             complement(<1..749)
                     /locus_tag="AC159P_10650"
                     /inference="COORDINATES: protein motif:HMM:NF024748.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HIR3695950.1"
                     /translation="MKLKNPDKAPARFLLACALALLFIPAARAQEVSRDGALRKKVEE
                     VIGASGAETVGVAFYDLETKRELLVNPDASFHAASTMKVPVMMEVFRQAHTGQLSLDA
                     SLDIRNQFKSIADGSFFSVSPEDDSEKTLYQKTGQAVAVRELVELMINASSNLATNVL
                     IQRVTPARIMVFMRKSGARNIQVLRGVEDGKAYERHMNNTTTAHDLSILLRRIAEGRA
                     VSKDASREMVEIMSRQKFNEGIPAGLPPSARV"
     gene            910..1578
                     /locus_tag="AC159P_10655"
     CDS             910..1578
                     /locus_tag="AC159P_10655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695951.1"
                     /translation="MRHLLFVSATAEAAEAAFEQARARDEQVLRLAEGGADGALQGLD
                     RRLAADGVNRVAHLGGALRARAERVPLGQHVIDEVAVMKGRARQIDRALPFEEVENLA
                     EDFFERVDDDEAEAAREATAAAAIHGERLHVFGAELPPAHRGEAAHHASLKVALRQVV
                     HDRHGDKRRKADEHRVGDDRPLAVDGGRRCVFHARSLTQRGKGRKGEKVERGKGEKEE
                     RLKG"
     gene            complement(1680..>2042)
                     /locus_tag="AC159P_10660"
     CDS             complement(1680..>2042)
                     /locus_tag="AC159P_10660"
                     /inference="COORDINATES: protein motif:HMM:NF024578.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HIR3695952.1"
                     /translation="GELPVQTSSANDTEEDGARPAATAEGTRLGVGVEPLTPEAAARL
                     KLPRDTQGLLVGTVAPDGPAAEAGVRRNDVIQEVNRQPVRTVEELRAALDRTGNRPAL
                     LLVTNEGGSGYVTVRLRR"
BASE COUNT          308 a          654 c          715 g          365 t
ORIGIN      
        1 acgcgcgccg aagggggcag gcccgccggg atgccctcgt tgaacttctg ccgcgacatg
       61 atctccacca tctcgcgcga cgcgtctttc gagacggcgc gcccctcggc gatgcggcgc
      121 agcaggatcg agaggtcgtg cgccgtcgtc gtgttgttca tgtggcgctc gtacgccttg
      181 ccgtcctcga caccgcgcag cacctgaatg ttgcgcgccc ccgacttccg catgaagacc
      241 atgatgcggg ccggcgtcac gcgctggatg aggacgttgg tggcgaggtt gctgctggcg
      301 ttgatcatca gctcgaccag ctcgcgcacg gcgaccgcct gtcccgtctt ttggtaaagc
      361 gtcttctccg agtcgtcctc gggcgagacg ctgaagaagc tgccgtcggc gatgctcttg
      421 aactggttgc ggatgtcgag cgacgcgtcg agcgagagct gccccgtgtg cgcctgccgg
      481 aaaacctcca tcatcaccgg caccttcatc gtgctcgccg cgtggaagga cgcgtccggg
      541 ttgacgagca gttccctctt cgtctccaaa tcgtagaacg cgacgccgac cgtctccgcc
      601 ccgctcgcgc cgatgacctc ttcgaccttc ttcctcaacg cgccgtcgcg gctcacctcc
      661 tgcgcgcggg cagcagggat gaagagcagc gcgagcgcgc aggcgaggag aaagcgtgcg
      721 ggagctttgt ccgggttctt caacttcatg acggcctcgg ggtcgtaggc ggcggctgct
      781 tgaggcgcgc ggcgagccgc gcgcgcaggt tgtcgaagtg gctttgcggg atgctgttga
      841 cgctgccctg gggcggttga cgacgccctc gatctcgcgc tcctcttcct cttcgaccga
      901 ctcgacggct tgcgccacct gctgttcgtc agcgcgaccg ccgaggcggc ggaagctgcc
      961 ttcgagcagg cgcgcgcccg cgacgagcag gttcttcgac tggcggaagg tggcgccgac
     1021 ggcgctctcc aagggcttga tcggcgcctc gcggcagacg gcgtcaatcg cgtcgcgcac
     1081 ctcggcggcg ctcttcgtgc ccgcgccgag cgcgtcccac tcggccagca cgtaatcgac
     1141 gaggtagccg tgatgaaagg ccgcgctcgt cagatcgacc gcgcgcttcc attcgaggaa
     1201 gtagaaaacc ttgcggaaga tttttttgag cgggtagacg atgacgaggc cgaggcagcc
     1261 cgcgaggcaa ccgccgccgc cgcgatccac ggcgagcgtc tccacgtctt cggcgccgag
     1321 ttgccgcccg cgcatcgcgg cgaggctgcg caccatgcgt cgctgaaagt agctcttcgc
     1381 caggtcgtcc acgaccggca cggggataag cggcgtaagg ccgatgagca ccgcgtgggt
     1441 gatgatcgtc ctctggctgt tgacggcggg cggcggtgcg ttttccacgc gcgcagttta
     1501 acgcagaggg gaaaaggcag aaagggcgaa aaggttgaaa ggggaaaagg cgaaaaggaa
     1561 gagaggttga aaggataaga ggttgagagg ggaagaggcg aaaagggagg atgagaggca
     1621 cgctgcttct tgtcttccct tttcgccctt tccccttttc cccttttcgc ccctcttctt
     1681 caacggcgga ggcggacggt gacatagccg ctgccgccct cgttggtgac gagcaggagc
     1741 gcggggcggt tgccggtgcg gtcgagcgcg gcgcgcagct cttcgacggt gcggacgggt
     1801 tggcggttga cctcctgaat cacgtcgttg cggcggacgc ccgcttcagc cgcggggccg
     1861 tcgggggcga cggtgccgac gagcagccct tgcgtgtcgc gcgggagctt gagccgcgcg
     1921 gcggcctcgg gcgtcagcgg ttcgacgccg acgccgagcc tcgtcccttc agcggtcgcg
     1981 gcgggtcgcg cgccgtcctc ttcggtgtcg ttcgcgctcg acgtttggac gggcagctcg
     2041 cc
//
LOCUS       DBNGOH010000549         6090 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2829980, whole genome shotgun sequence.
ACCESSION   DBNGOH010000549 DBNGOH010000000
VERSION     DBNGOH010000549.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6090)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6090)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6090
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2829980"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(29..655)
                     /gene="plsY"
                     /locus_tag="AC159P_10665"
     CDS             complement(29..655)
                     /gene="plsY"
                     /locus_tag="AC159P_10665"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HIR3695953.1"
                     /translation="MPPSLIILLAYLIGSIPFGYLIVKLRGEGDVRETGSGGTGATNV
                     TRRAGRAAGVLTLVLDAAKGAVAVLLARWLLTDDFGVNWWVAGAAVAAIAGHVFPVWL
                     GFRGGKGVATGLGVFACLAPLAVLCVAPVFVAVVWATRYVSLGSITASAALPLFVRLF
                     GGRGDATATTTTLAPLLAAAVAGAALIIFMHRANIGRLLRGTENKLKL"
     gene            complement(796..1446)
                     /locus_tag="AC159P_10670"
     CDS             complement(796..1446)
                     /locus_tag="AC159P_10670"
                     /inference="COORDINATES: protein motif:HMM:NF018446.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA];
                     GO_function: GO:0022841 - potassium ion leak channel
                     activity [Evidence IEA];
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HIR3695954.1"
                     /translation="MESNEKESGLSFERVVFFSDAVFAIVITLLVLEIKVPHIEGHTE
                     QGVLQGLFHLLPKFIGFVMSFFIVGVMWIEHHRIFRFIERYDTGLLLRNLTFLLSVSF
                     VPFPTALFSEYYWSRTAFILYAATFGVVALTKLWVWNYAARRPALLGPSSDAATVRRI
                     SRRSLAVPIACAAAIALSFVSVFLAPLGFTLIPLVARALDPGARGGGELAEEAPST"
     gene            complement(1585..2820)
                     /locus_tag="AC159P_10675"
     CDS             complement(1585..2820)
                     /locus_tag="AC159P_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013579420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HIR3695955.1"
                     /translation="MLTAEIIAVGSELLTPHRTDTNSLWLTERLNSVGIEVKLKTVVG
                     DDDARLKETISDALKRSGVVITTGGLGPTEDDITRKIAARALGRRLMPDERVLEDIRA
                     KFLHWGRKMPEINARQAMVLEGATVLDNPNGTAPGLYVEHEGRVVALLPGPPRELKPM
                     FDREVLPRLAARAGGVRVVRRVLRVTGMGESAVDERIAPVYRQYENPQTTILFTNSEI
                     EIHLTAQGATEQEAELLLDGLAGQIEERLGDAVFAFRGEKMEEVVGLRLAVAGFTLAT
                     AESCTGGLVAERLTEIPGSSHYFMEGVVTYSNESKTRLLGVPAELIEAHGAVSAEVAE
                     AMAEGVKRRAGVDFGLSVTGIAGPGGGTETKPVGLVYIALADDAHTEHRRLMLPGDRH
                     LIRWRSSQAALDLLRRRLI"
     gene            complement(2911..3675)
                     /locus_tag="AC159P_10680"
     CDS             complement(2911..3675)
                     /locus_tag="AC159P_10680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695956.1"
                     /translation="MQRLLAVVVIMAMLGVMGIPLTVIVFFATVVYFVWRAVQRSEQQ
                     DIKNIFDFYIAANEILRDDEKRWYGFEINQVVGEGERVMHLLPDPPPLVYFALGALYH
                     RAGDHESAVEQLSYVTENEQGDENYRFQPSPELRRYVEVLRRIERDPSEAPQTMAAIR
                     SLERARRTRAAAMLDQSRVRVGEGAVHASIPPAPHAHEHGRAHKAEDITLSSLAGKRH
                     QPPQHQPPPAPPTTMTPPAPIAEVLRDLYEEEKKTA"
     gene            complement(3781..4848)
                     /locus_tag="AC159P_10685"
     CDS             complement(3781..4848)
                     /locus_tag="AC159P_10685"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HIR3695957.1"
                     /translation="MTGQVKRIAVIPGDGIGPEVTAEAVRALELVGETHGVRLDFKHF
                     DWGADKFLSEGVSLPEGALDMLAREFDAILSGAFGDPRVPSNQHAEDILMGMRRGLDL
                     YINMRPVRLLDSRLTPLRDRGVRDIDFVVFRENTEGAYCGAGGFLKKGTADEVATQEE
                     FNTRRGVERIIIAAFEYARSRGRKRVTMADKSNVQRFGGDLWQRVFKEVAARYADIEA
                     NHQYADATAMFMVQNPAQYDVLVTNNLFGDILTDLGAALQGGLGLAASGNIHPGRVSL
                     FEPVHGSAPPLAGKNQANPAGAILSSALMLEYLDFSEAAAALERAVRECIAAGETTRD
                     LGGALSTTEAGARIRERIKRK"
     gene            complement(4866..5306)
                     /locus_tag="AC159P_10690"
     CDS             complement(4866..5306)
                     /locus_tag="AC159P_10690"
                     /inference="COORDINATES: protein motif:HMM:NF012791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR3695958.1"
                     /translation="MLKATDLLVPVDFSPTSINAVRVAVGIAAPGGDITLLHVIDGHF
                     IEDAVAAAFGSTEEITARLRAQAEANFTNALEGIETGEVNVERMVVVGAPFVEILKIA
                     RDLDLPMIVMGVRGRSTGAEELLFGSTAEKVLRGTRIPVLCVPF"
     gene            complement(5440..6030)
                     /locus_tag="AC159P_10695"
     CDS             complement(5440..6030)
                     /locus_tag="AC159P_10695"
                     /inference="COORDINATES: protein motif:HMM:NF012339.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HIR3695959.1"
                     /translation="MSVNVEWKTEGEETLPGGIVAEGTYLWDAAKRLGARLPAECGGK
                     GECDTCAVVVVEGATLLSSLTDAERRLLSPERLAEGERLACQAKIERGGDLVLRPVPQ
                     TERKETADESVKDFRKEVREMPLQKKLATLVEFEAITAYQTLNKIFDLPFNVVEKGLD
                     LMAVRGRALSQREREARRPTEHRAATEEPAKEGEQS"
BASE COUNT          992 a         2072 c         2039 g          987 t
ORIGIN      
        1 gctatattcc gacttctgac ttccgacttc atagtttcag tttgttctcc gtcccgcgca
       61 gcagcctgcc gatgttggcg cggtgcatga agatgatgag cgcggccccc gcgacggcag
      121 ccgcgaggag cggcgcgagc gtggttgtcg tcgccgtcgc atcgccccga ccgccgaaga
      181 gccggacgaa aagcgggagc gccgccgacg ccgtgatcga gccgagcgag acgtagcgtg
      241 tcgcccacac gacggcgacg aagacgggcg cgacgcacaa caccgcgagc ggcgcgaggc
      301 aggcgaagac gccgaggccc gtcgccacgc ccttgccgcc gcggaagccg agccacacgg
      361 ggaagacgtg accggcgatg gcggccaccg ccgcccccgc gacccaccag ttgacgccga
      421 agtcgtctgt caacagccag cgcgcgagca agaccgcaac cgcgcccttc gctgcgtcga
      481 gcacgagcgt caggacgccc gccgcgcgcc ccgcgcgccg cgtgacgttg gtcgcgcccg
      541 tcccgcccga ccccgtctcg cgcacatcgc cctcgccgcg cagcttcaca atcaagtacc
      601 cgaatggtat cgacccgatc aggtacgcga gcaggatgat gagggagggc ggcatcggga
      661 ctcaatcgca aaggctgacg ggaaggcggc ggcgcgaccc gccgaatata gcagcggcgt
      721 gagggcgcga caaggaaggg cgcggcgtcc ggcccttccc ttcgacggcg ttcgaggacg
      781 agggcgggca ccccttcacg tggaaggggc ttcctcggcg agttcgccgc cgccgcgcgc
      841 gccggggtcg agtgcgcgtg cgacgagcgg gatcagggtg aagccgaggg gcgcgaggaa
      901 gacgctgacg aacgagagcg cgatggccgc ggcgcacgcg atgggcacgg ccagcgagcg
      961 gcggctgatg cggcgcaccg tcgcggcgtc ggacgagggg ccgagcagcg cgggcctgcg
     1021 cgcggcgtag ttccagaccc agagcttcgt cagcgccacg acgccgaacg tcgcggcgta
     1081 gaggatgaac gccgtgcggc tccagtagta ttcgctgaag agcgcggtcg ggaaagggac
     1141 gaacgagacg ctgagcagga aggtgaggtt gcgcagcagc agtcccgtgt cgtaccgctc
     1201 gatgaagcgg aagatgcggt ggtgctcgat ccacatcacg ccgacgatga agaagctcat
     1261 cacgaacccg atgaacttcg gcagcaggtg gaagagacct tgcagcacgc cctgctccgt
     1321 gtgcccctcg atgtgcggca ccttgatttc cagcacgagc agcgtgatga cgatggcgaa
     1381 caccgcgtcg ctgaagaaca cgacgcgctc gaaactgagt ccggactcct tctcattcga
     1441 ttccataaag tctcatacgg gccgacgggc agaaagtttt ccgagcgacg cggcgacgaa
     1501 agcgaggagg agcgaatacg tcttgatgtg aaatgaagta cgcgaaaccc gccgcgaatc
     1561 ggcggactgc ttcgcggtcg tcgttcatat cagccgcctt cgcaacaagt cgagcgcggc
     1621 ctgcgaggag cgccagcgga tgaggtggcg gtcgccgggg agcatgaggc ggcggtgttc
     1681 ggtgtgcgcg tcgtcggcga gcgcgatgta gaccaggccg acgggcttcg tttcggtccc
     1741 gccgcccggc cccgcgatgc ccgtcacgga gagtccgaag tccacgcccg cgcgccgctt
     1801 cacgccctcg gccatcgcct ccgcgacctc ggcgctgacg gcgccgtgcg cttcgatcaa
     1861 ctccgcgggc acgccgagca gccgtgtctt cgactcgttc gagtaggtga cgacgccctc
     1921 catgaagtag tgcgaagagc cggggatttc cgtcaggcgc tcggcgacaa gaccgcccgt
     1981 gcagctctcg gcggtggcga gcgtgaagcc cgcgacggcg agacgcaagc ccacgacctc
     2041 ttccatcttc tcgccgcgga aggcgaagac ggcgtcgccg agtcgctcct cgatctgccc
     2101 ggcgaggccg tcgagcagca gctcggcctc ctgctcggtg gcaccctgcg cggtcaggtg
     2161 aatctcgatt tcgctgttgg tgaacaggat cgtggtctgc gggttctcgt actggcggta
     2221 gaccggcgcg atgcgctcgt ccaccgccga ctcgcccatg cccgtcacgc gcagcacgcg
     2281 gcgcacgacg cgcacgccgc ccgcgcgcgc ggccagacgc ggcagcacct cgcggtcgaa
     2341 catcggcttc agctcgcgcg gcggtcccgg caagagcgcg accacgcggc cttcgtgttc
     2401 gacgtagagg ccgggcgccg tcccgttcgg gttgtcgagc accgtcgcgc cttcgagcac
     2461 catcgcctgc cgcgcgttga tctccggcat cttgcggccc cagtgcagga acttcgcgcg
     2521 gatgtcttcg agcacgcgct cgtcgggcat gagcctgcgg cccaacgcgc gcgcggcgat
     2581 tttgcgcgtg atgtcgtcct ctgtcgggcc gaggccgccc gtggtgatga cgacgccgga
     2641 gcgcttgagc gcgtcgctga tggtctcctt gagccgtgcg tcgtcgtcgc cgacgaccgt
     2701 tttgagcttg acctcgatgc cgacgctgtt cagccgctcg gtcagccaca ggctgttggt
     2761 gtcggtgcgg tgcggcgtca aaagttcgga gccgacggcg atgatctcag cggtgagcat
     2821 gaatctacgg gtgtcgatgc gggcgctaca cacaaaagcg gcgaccgaag tttcacaaca
     2881 gtcgccgccc ttcgtctcga acggtctcgc tcacgccgtc ttcttctctt cctcgtacag
     2941 gtcgcgcagc acttcggcga tgggcgcggg cggcgtcatc gtcgtgggcg gcgcgggcgg
     3001 aggctgatgc tgcggcggct ggtggcgctt gcccgcgagc gagctgaggg tgatgtcctc
     3061 ggccttgtgc gcgcgcccgt gctcatgcgc gtgcggcgcg ggcgggatgg aggcgtggac
     3121 ggcgccctcg ccgacgcgga cgcggctctg gtcgagcatg gcggcggcgc gcgtgcgccg
     3181 cgcgcgctca aggctgcgaa tggcggccat cgtctgcggc gcctccgagg ggtcgcgctc
     3241 gatgcggcgc agcacctcga cgtaacgccg caactcgggc gacggctgga agcggtagtt
     3301 ctcgtcgccc tgctcgttct cggtcacgta ggagagctgc tcgaccgccg actcgtggtc
     3361 gcccgcgcgg tggtagagcg cgccgagcgc gaagtagacg agcggcggcg ggtcgggcaa
     3421 caggtgcatc acgcgctcgc cctcgccgac cacctggttg atctcgaagc cgtaccagcg
     3481 cttctcgtcg tcgcgcagga tttcgttggc cgcgatgtag aagtcgaaga tgttcttgat
     3541 gtcctgctgc tcggagcgct gcaccgcgcg ccagacgaag tagacgaccg tggcgaaaaa
     3601 gacgatcacc gtcagcggga tgcccatcac gccaagcatc gccatgatga cgaccaccgc
     3661 cagaaggcgc tgcactgttt cctgcccctt agtcataaag cacaagtgat gaatgcggaa
     3721 tgttgaacga tgaataaaat acaagctgtc ttctattcat cgttcatcat tccgcattca
     3781 tcatttcctc ttgatccgct cgcggattct cgcgcccgct tcggtcgtgg agagggcgcc
     3841 gccgaggtcg cgcgtcgtct cgccggcggc gatgcattcg cgcacggcgc gttcgagcgc
     3901 cgccgccgcc tcgctgaagt cgaggtattc tagcatcaag gccgaactga gaatagcccc
     3961 tgccgggttg gcctgatttt taccggcgag cggcggcgcg ctgccgtgca ccggctcgaa
     4021 cagggagacg cggcccggat ggatgttgcc cgaggcggcg aggccgaggc cgccctggag
     4081 cgccgcgccg aggtcggtca ggatgtcgcc gaacaggttg ttagtcacca ggacatcgta
     4141 ctgcgccggg ttctgcacca tgaacatcgc ggtcgcgtcg gcgtactggt ggttcgcctc
     4201 gatgtccgcg tagcgcgccg cgacctcctt gaagacgcgc tgccagaggt cgccgccgaa
     4261 gcgctggacg ttcgacttgt cggccatcgt cacgcgcttg cgaccgcgcg accgcgcgta
     4321 ctcgaacgcc gcgatgatga tgcgctcgac accccgccgc gtgttgaact cctcctgcgt
     4381 ggccacctcg tccgccgtcc cttttttgag gaagccgccc gcgccgcagt aggcgccctc
     4441 tgtgttctcg cggaagacga cgaagtcgat gtcacggacg ccgcggtcgc gcaaaggcgt
     4501 gagacgcgaa tcgagcaggc ggacgggccg catgttgatg taaagatcga ggccgcgccg
     4561 catccccatc aggatgtctt cggcgtgctg gttcgagggg acgcgcgggt cgccgaacgc
     4621 gcccgacagg atcgcgtcga actcgcgcgc cagcatgtcg agcgcgcctt cgggcagcga
     4681 cacgccctcg ctcaaaaact tgtccgcgcc ccagtcgaag tgcttgaagt cgagccggac
     4741 gccgtgcgtc tcgccgacga gttcgagcgc gcggacggcc tccgccgtca cctccggccc
     4801 gatgccgtcg ccggggatca ccgcgatgcg tttcacttga cccgtcatca acaaaccacc
     4861 ccgccctaga acggcacgca gagcacgggg atgcgcgtcc cgcgcaagac cttctcggcg
     4921 gtcgagccga agagcagctc ctccgcgccg gtcgagcgcc cgcgcacgcc catcacgatc
     4981 atcggcaaat cgaggtcgcg cgcgatcttc agaatctcga cgaacggcgc gccgaccacg
     5041 accatgcgct cgacgttgac ctcgcccgtc tcgatgcctt cgagcgcgtt cgtgaagttc
     5101 gcctcggcct gcgcgcgcag ccgcgccgtg atctcctcgg tcgagccgaa ggcggcggcc
     5161 accgcgtcct cgatgaagtg gccgtcgatc acgtggagca gcgtgatgtc gccgcccggc
     5221 gcggcgatgc cgaccgcgac acgcaccgcg ttgatcgagg tcggcgagaa gtccaccggg
     5281 acgagaaggt ctgtcgcttt caacatgact cacctgacga ttgcggattg cggattgcgg
     5341 attgcggatt agtcggacga tgaaagtcga actttcgaac tacggcgtcg agcctgagca
     5401 gcacgccgat ccaaaatccg caatccaaaa tccgcgatcc tacgactgct cgccttcctt
     5461 cgcaggctct tcggtcgcgg ccctgtgctc ggtggggcgg cgcgcttcgc gctcgcgctg
     5521 cgagagcgcg cgcccgcgca cggccatcag gtcgagaccc ttctcgacga cgttgaacgg
     5581 gaggtcgaag attttgttga gcgtctggta ggccgtgatc gcctcgaact cgacgagcgt
     5641 cgccagcttc ttctggagcg gcatctcgcg gacctctttg cggaaatcct tgaccgactc
     5701 gtccgccgtc tccttgcgct cggtctgcgg cacgggccgc aaaacgaggt cgccgccgcg
     5761 ctcgatcttc gcctgacacg ccagccgctc gccctccgcc agccgctccg ggctgagcag
     5821 ccgccgctcc gcgtccgtca gcgacgagag cagcgtcgcc ccctccacga ccacgaccgc
     5881 gcacgtgtcg cactcgccct tccccccgca ctccgccggc agccgcgcgc ccagacgctt
     5941 cgccgcgtcc cacaggtacg tgccctccgc gacgatgccg cccggcagcg tttcctcgcc
     6001 ttccgtcttc cactccacgt tgacgctcat ccgctatacc tcccgccacg agcttgcttg
     6061 ttgtattgat tccgttgaga aaagtatacc
//
LOCUS       DBNGOH010000550         3357 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_998280, whole genome shotgun sequence.
ACCESSION   DBNGOH010000550 DBNGOH010000000
VERSION     DBNGOH010000550.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3357)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3357)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3357
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_998280"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(269..523)
                     /locus_tag="AC159P_10700"
     CDS             complement(269..523)
                     /locus_tag="AC159P_10700"
                     /inference="COORDINATES: protein motif:HMM:NF018961.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin"
                     /protein_id="HIR3695960.1"
                     /translation="MGENVFKAVEIVGTSDQSFSHAIEVAVSRARRTLRELSWFVVEE
                     MRGGLQKGRLEYQVTLRVFFKLEGDDEVTATGAATDDAGQ"
     gene            1221..1664
                     /locus_tag="AC159P_10705"
     CDS             1221..1664
                     /locus_tag="AC159P_10705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695961.1"
                     /translation="MLKRSWAALAFVVACAVSGVAQEPPAFSIPFRELESYKKAGREW
                     RNIVVDQKIKRDELVRLARELHAAYPETPFRIFTDDGKFREFMQHDLRRSDPAYAYPE
                     EWAERHYVADINGIWRRSGHRWVLYPAGPRGRKFVPQGETAIAEL"
     gene            complement(1770..2015)
                     /locus_tag="AC159P_10710"
     CDS             complement(1770..2015)
                     /locus_tag="AC159P_10710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695962.1"
                     /translation="MGKVTRSKAASNASKALRTSTSKTVRTVAASALTQSKAPGERTT
                     RKVATAASKVLSDGRVSKAAKSAAGSVLAQARGKKKR"
     gene            complement(2212..2943)
                     /locus_tag="AC159P_10715"
     CDS             complement(2212..2943)
                     /locus_tag="AC159P_10715"
                     /inference="COORDINATES: protein motif:HMM:NF021759.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT-like domain-containing protein"
                     /protein_id="HIR3695963.1"
                     /translation="MADAQPLIETLFREAFRQLAPKRYAPEVDVRYYPYAGLNHTIRS
                     RSGRVYVRVSDIFKVAPLNVHRALAFVLVSKLLRRRTPPFYERVYRDYACTPEVLRAS
                     DIARRNRGRKMISSAQGRHYDLERMFARLNRRYFDEQIEKPTLTWSQRRTRTILGHHD
                     GVHDTIVISKTLDSADVPEWFVEYILYHEMLHIKHPARVINGRRYYHTKAFRSEEQRY
                     PRYAEAQQWLERIVREHRTLRARAA"
BASE COUNT          584 a         1008 c         1124 g          641 t
ORIGIN      
        1 aggatgacca tgccgcggcg aatctgggtt gcgggaattg ccatagaaag atttcaactc
       61 cggttcgatt gaaatttttg aactgccgtg gcgagcggag tcgtgcgcgg gcctcatagt
      121 gaaagtcgca cggcctctcg caaacctcgt agtctagcga gcggcccggc cctttgtcca
      181 gcggaagaac agacggggag aaagggagac ggggagagag aagctgcgct gtcaaatgtc
      241 tccccgtctc cccatctccc tttctcccct actgccctgc gtcgtcggtc gcggcgccgg
      301 tggcggtcac ttcgtcgtcg ccttcgagct tgaagaagac gcggagcgtg acctggtatt
      361 cgagccgccc cttttgcagt ccgccgcgca tctcctcgac gacgaaccac gacaactcgc
      421 gcagcgtgcg gcgcgcgcgg ctgaccgcga cctcgatggc gtggctgaag ctctggtcgg
      481 aagtgccgac gatctcgacg gccttgaaga cgttttcgcc catgagtcct ccgtcaagca
      541 tccgaagatg cgaagcctcg aacttaatcc gcgagcttga attcggtgcg tggctagtgg
      601 ctggccggaa gctaacacgc gccggaaaag cgagtcaaga gatatgaaag aaaggagtgt
      661 gtacgagcgt tcggataagt tcgcaaaatc aagaacgggc cggcaacttg agaggcatcg
      721 gagggattga tgcttgtctc ttgctgccgg cccgctcttt tcggaggagc ccaccttcct
      781 gaccgttaga acgccgcaag ggggtaaaag tttcagaatt attttcttcc gtccaggtgc
      841 gttcctcgca tccccctttc cggatttagt ttacgggagc ggtcagttca cccttcgctg
      901 tcggcgagtg tccgacccgt ctcgcggcgg ccatctcagc cgcgcggcgc aggccgtagg
      961 cggacggggc gagaggcggg cgcccttgaa ggtcttcgga aaaccttgtc agcccaaact
     1021 cgccggtgat aaagtccata gtggttcgtc ctcgcgcttt acgcacctct ccctgagttc
     1081 tgcccgagac cttttccgct cagagtccag tcggttctct tgggtcccgg gccaggctag
     1141 acaacgcctt cataaccgtc cccgagcggg aggggcgggc gccccgcctt cgacctcatc
     1201 gcaagcgaaa ggaagaggcc atgctcaaac ggtcatgggc cgcgctcgcc ttcgtcgtcg
     1261 cgtgcgccgt ctccggcgtc gcgcaggagc cgccggcctt ctcaatcccc ttcagggaac
     1321 tcgaaagtta caagaaggcc gggcgcgagt ggcggaacat cgtcgtcgat cagaagatca
     1381 aaagggacga actggtgaga ctcgcccgcg aactccacgc cgcctatccc gagacgccct
     1441 tccgcatctt caccgacgac gggaagttcc gggagttcat gcagcacgac ttgcgccgct
     1501 ccgacccggc ttacgcctac cccgaggaat gggcggagcg gcactacgtc gcggacatca
     1561 acggcatctg gcgtcggagc gggcatcgct gggtgctcta cccggcgggg ccgaggggga
     1621 ggaagttcgt cccgcagggc gagaccgcga tagcagagtt gtgacggcga cgccggagcg
     1681 cacccccatc cgacttccat ggaaggctcc gtggcagggc gtccggtgcg ggtcgttgtc
     1741 aggcttcggc gcgcggcctc gacggcggct taccttttct tcttgccgcg ggcttgggcc
     1801 aggacgctgc cagccgccga tttggcggcc ttgctcaccc tgccgtcact cagcaccttg
     1861 gacgccgccg tcgccacttt cctggtggtc cgctctcccg gcgccttgct ctgcgtcagc
     1921 gcgctcgcgg cgaccgtcct gacggtcttg ctggtcgagg tgcgcaacgc cttcgaggcg
     1981 ttactggctg ccttgctgcg ggtgacttta cccatctcct ctacctcctg cttcagtaat
     2041 aagtcaggtc gcgcgcgtcg tgtgtttccg acggtgtagg cagggccgat tcgtaccctt
     2101 atatgagacc gcagggatgc gcggggaata tatgacgagg agggaggggt gtcaacgggg
     2161 agggctttgg cttctgccct ttttattccc gcggacgaaa tacgaaatcc ttcatgcggc
     2221 gcgggcgcgc agggtgcggt gctcgcgcac gatgcgttcg agccactgct gcgcctcggc
     2281 gtagcggggg tagcgctgct cctcggagcg gaacgccttg gtgtggtaat agcgcctgcc
     2341 gttgatgacg cgcgccgggt gtttgatgtg cagcatctcg tggtagagga tgtactcgac
     2401 gaaccactcg ggcacgtccg ccgagtcgag cgtcttgctg atgacgatgg tgtcgtgcac
     2461 gccgtcgtgg tggccgagga tggtgcgcgt gcggcgctgc gaccaggtga gcgtcggctt
     2521 ctcgatctgt tcgtcgaagt agcggcggtt gaggcgcgcg aacatccgtt cgaggtcgta
     2581 gtggcgaccc tgcgccgagg agatcatctt gcgaccgcgg ttgcggcgcg cgatgtcgga
     2641 ggcgcgcagg acttcgggcg tgcaggcgta gtcgcggtag acgcgctcgt agaagggcgg
     2701 cgtgcgccgc cgcagcagct tcgagacgag gacgaaggcg agcgcgcggt ggacgttcag
     2761 cggcgccacc ttgaagatgt ccgaaacgcg cacgtagacg cggcccgagc gcgagcggat
     2821 ggtgtggttc agcccggcgt aagggtagta gcggacatcg acttcagggg cgtagcgctt
     2881 cggggcgagc tgccggaagg cctccctgaa gagggtctcg atgagcggct gagcgtcggc
     2941 cataaagtaa accctgcgag ggtcgtcaag gcgcttgaaa tcgggtttca ccgccggacg
     3001 gtgggtcgct tccgctgccg aaaaaagcgc tgaagtccgg cgagttacct gatattacga
     3061 gccaactgcc gggggcgtca aatcggttcc gcacgcgcgc tgtaaaatct ttccctgcaa
     3121 tcagatgcac gcacggttcg gaaccgcacg cgcggaggtc gcggcgagcg aaactcgaac
     3181 ccccacgagt ccaacggttt aggccgcacc gaacgcaacc ggggaagtcg ccgtgaaccg
     3241 aggtcagtgg cgactccgag gtggatgccg tgagcggttt tggtcatccg gcttcggagc
     3301 ggcgtgttac cgagagggag gcgcgaaatg tttacggcgc aggccccatc tgctgtt
//
LOCUS       DBNGOH010000551         3995 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4479484, whole genome shotgun sequence.
ACCESSION   DBNGOH010000551 DBNGOH010000000
VERSION     DBNGOH010000551.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3995)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3995)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3995
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4479484"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            141..1094
                     /locus_tag="AC159P_10720"
     CDS             141..1094
                     /locus_tag="AC159P_10720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HIR3695964.1"
                     /translation="MTKSTPRIVSLLPSATEIVCALGLADALVGVTHECDFPPEVADK
                     PRLTRSRISHETMSSREIDHAVRSQLDGHGSIYDLDEGLLRELEPDLVITQELCEVCA
                     VSYKTVREAARVLDAEARVVSLEPNNIRDIFGTIRTVGELTGREEAAATLVGELSVRL
                     DALAVVLTEIERRPRTLMLEWLEPPFAPGHWVPEQVAFAGGDHTFGRAGEKSVRTTAE
                     EIEAYAPEVVVLIPCGYYKEDILRALPAARLPRGWHSLPAVERGEVWAVDAAAYFSRP
                     GPRVVEGAEVLARVLHPDIFGAPEERDAVRVSPDLMLVAQL"
     gene            1179..1505
                     /locus_tag="AC159P_10725"
     CDS             1179..1505
                     /locus_tag="AC159P_10725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020711688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HIR3695965.1"
                     /translation="MAYFLLQGAYTAEVWKTLVENPANRVEAIRPAIEKLGGRVESAF
                     FAFGDYDVVLIMEMPDNVSAAAFALAVAAGGAFKSHKTTPLMSMEEGIEAMKKAGGTG
                     YRPPGS"
     gene            1672..3402
                     /locus_tag="AC159P_10730"
     CDS             1672..3402
                     /locus_tag="AC159P_10730"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0071897 - DNA biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HIR3695966.1"
                     /translation="MGRAKRAGQTRAANPAAEGAAAKRGTLRRFAEVAEQVAATTKKL
                     EKAALLGAYFDDLDDEDLARAARYFAGHQFALNDERKTNVGGALLREALGEVTGLDVE
                     NLRPRYVRLGDAGDVAFEAFTETRGANTSPALTLAETAALVELLSGTRGTKNKQTILV
                     GALASATPLEAKYLVKLLAGDLRIGLKEGLVEDAVARAFAQPLAAVAQVNMLTGDIGE
                     TAVRARRGLLEGVGLRLFHPLKFMLATAAADLSDVARTMPEEFFVEDKFDGIRAQAHA
                     QEGRVALYTRTLDEVTHRFPELVAPLKNLTTDAVVDGEIVPVRGEKILPFQELQKRLG
                     RKTVSDELLAAVPVAFVAYDLLYASGRVLTDEPLGERRRVLESLFEHDAAATVRLSEL
                     KRVNDVALLDEEFDAARARGNEGLMIKDPRSSYKPGKRGREWLKLKRALATLDVVVTA
                     VEVGHGKRRHLLSDYTFAVRRSEEDPELLNVGKAYSGLTDAELTELTEWFRAHTLQEF
                     AHGRVRVVEPKIILEVTFDRVQESRRHKSGYALRFPRILRLREDKSVAEIDTLDAVRA
                     LAADAPGREV"
     gene            3462..3932
                     /locus_tag="AC159P_10735"
     CDS             3462..3932
                     /locus_tag="AC159P_10735"
                     /inference="COORDINATES: protein motif:HMM:NF017016.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA];
                     GO_process: GO:0000921 - septin ring assembly [Evidence
                     IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HIR3695967.1"
                     /translation="MTSDDDTTPAGRVQVRIFDRVYMLRAGGGDEGYVRRVARIVDER
                     MQQVSELLTTSDVAKVAVLAALNIAGELEELRARHEQGARAAEAEGAEEPARPAAEVA
                     RDERRSWFEEIFDGEFAQKKNSDERLSSLVSSKLQRHRPAGGGDTERAEPEGEN"
BASE COUNT          702 a         1293 c         1443 g          557 t
ORIGIN      
        1 caaatatcga atccgagagt tcttcactgc ctactgccta ccgcctactg cctaccgcct
       61 tctgcattgt cttgacacgg gcgccgccga tgcgcgaacc tcgcgggcaa tcgagagagt
      121 tgacggggag agaggacttt atgacgaaga gcacgccgcg catcgtctcg ctcctgcctt
      181 cggcgaccga gatcgtgtgc gcgctcgggc tggcggacgc gctggtcggc gtcacgcacg
      241 agtgcgactt cccgccggag gtcgcggaca agccgcggct cacgcgcagc cgcatctcgc
      301 acgagacgat gtcgagccgc gagatcgacc acgccgtgcg ctcgcagctc gacgggcacg
      361 gctcgatcta cgacctcgac gaagggctgc tgcgcgagct ggagccagac ctcgtcatca
      421 cgcaggagct gtgcgaggtg tgcgccgtca gctacaagac ggtgcgcgag gcggcgcgcg
      481 tgctcgacgc cgaagcgcgc gtcgtctctc tggagccgaa caacatccgc gacatcttcg
      541 gcaccatccg caccgtcggc gagctgaccg ggagggaaga ggccgcggcc acgctggtcg
      601 gcgagctgtc cgtgcgcctg gacgcgctgg ccgtcgtctt gaccgagatc gagcgcaggc
      661 cgcgcacgct gatgctcgaa tggctggagc cgcccttcgc gcccggccac tgggtgcccg
      721 agcaggtcgc gttcgcgggc ggcgaccaca cgttcggtcg cgccggcgag aagtccgtcc
      781 ggacgaccgc cgaagagatc gaggcgtacg cgcccgaggt ggtcgtcctc atcccgtgcg
      841 gctactacaa ggaagacatc ctgcgtgcgt tgcccgcggc gcgcctgccg cgcggctggc
      901 actcgctgcc cgcggtcgag cgcggcgagg tgtgggccgt ggacgccgcc gcctacttca
      961 gccgccccgg cccgcgcgtc gtcgagggcg ccgaagtcct cgcccgcgtg ctgcacccgg
     1021 atatcttcgg cgcgcccgag gagcgagacg ccgtgcgcgt cagcccagac ctgatgctcg
     1081 tcgcccaact gtagagtcag gcgcgatgcc tgacggaacc tgctcacgca ttcagtgctg
     1141 tccctcgccg gtactccacg gaacggagga gtgaaaagat ggcgtacttc ttgttgcagg
     1201 gagcctacac ggctgaggtt tggaaaacgc tggtggaaaa tccggcgaac agggtcgagg
     1261 ccatacgccc ggccatcgaa aaattgggcg ggcgggtcga gagcgccttc ttcgccttcg
     1321 gcgactatga cgtggtgctc atcatggaga tgcccgacaa cgtgagcgcc gcggccttcg
     1381 ctctcgccgt cgccgcgggc ggagccttca agtcgcacaa gacgaccccg ctgatgagca
     1441 tggaggaggg gatcgaggcg atgaagaaag caggcgggac gggataccgc ccgccgggca
     1501 gctagaagcc gcgcgggcgc gaacgaactc gctgctcgac gaggtgccgt gaacgaaaga
     1561 tcagcggact cgaagccgcc gtcgccgccg cggaaaccga aggcgacggg tgaagagtga
     1621 tgagaagcac gctgtttctc gtcactcatc acgcgtcgtt cgaaaagcat catgggtcga
     1681 gcaaaaaggg caggacaaac gcgggcggcg aatccggcgg cggaaggcgc ggcggccaag
     1741 cgcggcacgc ttcggcgctt cgcggaggtg gccgaacagg tcgcggcgac gacgaagaag
     1801 ctggagaagg cggcgctgct cggcgcgtac ttcgatgatt tggacgacga agacctcgcg
     1861 cgcgcggcgc gctacttcgc gggccaccag ttcgccctga acgacgagcg gaagacgaac
     1921 gtcggcggcg cgctgctgcg cgaggcgctc ggcgaggtga cgggcctcga cgtggagaac
     1981 ctgcgcccgc gctacgtgcg gctcggcgac gcgggcgacg tggccttcga ggcgttcaca
     2041 gagacgcgtg gcgcgaacac ctcgcccgcg ctcacgctcg cggagaccgc ggcgctcgtc
     2101 gagctgctga gcgggacgcg cgggacgaag aacaagcaga cgatcctcgt cggcgcgctc
     2161 gcttcggcga cgccgctcga agcgaagtat ctggtcaagc tcctcgccgg cgacctgcgc
     2221 atcgggctga aggaggggct ggtcgaagac gccgtggcgc gcgccttcgc gcagccgctc
     2281 gcggcggtcg cgcaggtcaa catgctgacg ggcgacatcg gcgagacggc ggtgcgcgcc
     2341 cgccgcgggc tgctcgaagg cgtgggcctg cggctcttcc acccgctcaa gttcatgctc
     2401 gccacggcgg cggcagacct ctcggacgtg gcgcgcacga tgcccgaaga gttcttcgtc
     2461 gaagacaagt tcgacggcat ccgcgcgcag gcgcacgcgc aagaaggccg cgtcgcgctc
     2521 tacacgcgca cgctcgacga ggtgacgcac cgcttccccg aactcgtcgc gccgctgaaa
     2581 aatctaacga ccgacgcggt tgttgacggc gagatcgtgc ccgtccgcgg cgagaagatt
     2641 ctcccgtttc aggagctgca aaagcggctc ggtcgcaaga cggtctcgga cgagctgctc
     2701 gcggcggtgc ccgtcgcctt cgtcgcttac gatctgctct acgcgtcggg tcgggtgctg
     2761 acggacgaac cgctcggcga aagaaggcgc gtgctcgaaa gtctcttcga acacgacgcg
     2821 gcggcgaccg tgcggctgtc ggagttgaag cgcgtgaatg atgtcgcgct gctcgacgag
     2881 gagttcgacg cggcgcgcgc gcgcggcaac gaggggctga tgatcaaaga cccgcgctct
     2941 tcgtacaagc cgggcaagcg cgggcgcgag tggctgaagc tgaagcgcgc gctggcgacg
     3001 ctggatgtcg tcgtcaccgc ggtcgaggtc gggcacggca agcgccgtca cctgctgtcc
     3061 gactacacct tcgccgtgcg gcgctcggag gaagacccgg agctgctcaa cgtcggcaag
     3121 gcttactcgg ggttgaccga cgccgagctt acggagctga ccgagtggtt ccgcgcgcac
     3181 accttgcagg agttcgcgca cgggcgcgtg cgcgtcgtcg agccgaagat tattctggaa
     3241 gtcaccttcg accgcgtgca ggaatcccgc aggcacaaga gcggctacgc cctgcgcttc
     3301 ccgcgcatcc tgcgcctgcg cgaagacaag tcggtcgccg agatcgacac gctcgacgcg
     3361 gtgcgcgcgc tggcggcgga cgcgccgggg cgggaggtgt aagtcaagga gaagcgagga
     3421 gaccggcggc agcgcgggcc gcgcgccggg actggagaaa catgacgagt gacgacgaca
     3481 cgactcccgc cgggcgggtg caagtccgca tcttcgaccg cgtgtacatg ctccgcgcgg
     3541 gcggcggcga cgagggctac gtccgccgcg tcgcgcggat cgtcgatgag cggatgcagc
     3601 aggtctccga actgctcacg accagcgacg tggcgaaggt cgccgtgctc gccgcgctga
     3661 acatcgcggg cgagttggag gagctgagag cgcgccacga gcagggcgcg cgcgcggcgg
     3721 aagccgaagg cgcggaggaa cccgcacggc ccgccgccga ggtcgcgcgc gacgagcgcc
     3781 gttcgtggtt cgaggagata ttcgacggcg agttcgcgca gaagaagaac agcgacgagc
     3841 ggctgagcag cctcgtctct tccaagctcc aacggcaccg cccggcgggc ggcggcgaca
     3901 cagaacgggc cgagccggag ggagagaatt agcgcagtgc gcggacgagc ttaaaggaag
     3961 ttcgacttta acgcggagtg cgcggagaag cgcag
//
LOCUS       DBNGOH010000552         2089 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3013740, whole genome shotgun sequence.
ACCESSION   DBNGOH010000552 DBNGOH010000000
VERSION     DBNGOH010000552.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2089)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3013740"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(158..1960)
                     /locus_tag="AC159P_10740"
     CDS             complement(158..1960)
                     /locus_tag="AC159P_10740"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HIR3695968.1"
                     /translation="MPVFRHTPAGRVYGSGRAFCFLTALAVVLACAAPPARAQGYERE
                     IEAGETVELRVQNRLGRVTVIAAEEQQKKVSVRAVSAGAAISEKDVRVESGGGRVVIE
                     VERAGASKPDGDGRKLSVSPARAERERIDVTVRIPVRSRVVVETEAGAVDVVGNVAEA
                     EVKTGTGTIRADVPLDALRYNFKWTLARPRYYSEVALEKVREKRGGTFEISGRFGAKK
                     PEQDKAVRLDLETARGVVLFGVADEGSVPADLRERTLTEAARAVISSGDQDLTNAIRR
                     IAPKLVGEYAETLPPRSTEPTLGARRDPFNVRTPAGGALARLQASVTDRQGRAIGGLS
                     HKDFSVVEDNQPRTIRDVQPSSAPFNLVLLLDVSGSVEERLDFIRKAALNFIQTVGPR
                     DRIAIVSFRDDVQLISDFTTDRAQLAERIKDIQAGGGTALYDSIAYALVHTLKPLRGE
                     RTGIVILSDGDDNKSFIPFNSIAEAVVETGALVYPLYVPSGLIPSSSAPVANASIDPM
                     RNRYLTLTSRADAEGRRLAEVSGGVYYPVTRIDQLQRAYDDVVEQLRAAYTITYETAA
                     NVRPEARVRVRVAREGASVRLGPAVGVSATAATP"
BASE COUNT          309 a          703 c          750 g          327 t
ORIGIN      
        1 cggcgggttc cggtcaacct gcgcgccggc gccgggagac aggacagccg ccgcgagcga
       61 cgaggccgcg acgagcacca acagttcgcg cttccgcaaa aacatcttcg gcccctttga
      121 cggtcgaaat tttcggcggg ggaagttctt cgatgcccta cggcgtggcc gctgttgccg
      181 agacgccgac cgcgggaccg aggcgcacgc tcgcgccctc gcgcgcgacc cgcacacgca
      241 cacgcgcctc gggccgcacg ttcgccgccg tctcgtaggt gatggtgtag gcggcgcgca
      301 gttgctcgac cacgtcgtcg taggcgcgct gcaactggtc gatgcgcgtc acggggtagt
      361 agacgccgcc cgagacctct gcgagccggc gcccctcggc gtcggcgcgc gaggtgagcg
      421 tcagataccg gttgcgcatc gggtcgatgg aggcgttcgc cacgggcgcg ctcgaagagg
      481 ggatcagccc cgaagggaca tagagcgggt agacgagcgc gcccgtctcg acgacggcct
      541 cggcgatgga gttgaagggg atgaaagact tgttgtcgtc gccgtcggag aggatgacaa
      601 tccctgtgcg ctcgccgcgc agcggcttca gcgtgtggac gagcgcgtag gcgatggagt
      661 cgtaaagggc ggttccgccg ccggcctgga tgtccttgat gcgctcggcg agctgcgcgc
      721 ggtcggtcgt gaagtcggag atgagctgca cgtcgtcgcg gaacgagacg atggcgatgc
      781 ggtcgcgcgg cccgaccgtc tggatgaagt tgagcgcggc cttgcggatg aagtcgagcc
      841 gctcttcgac gctgcccgac acgtcgagca ggaggacgag gttgaagggc gccgacgaag
      901 gctgcacgtc tctgatcgtg cgcggctggt tgtcctcgac gacggagaag tctttgtggg
      961 agaggccgcc gatggcgcgg ccctgccggt cggtgacgct cgcctgaagg cgcgcgagcg
     1021 cgccgcccgc gggcgtgcgg acgttgaagg ggtcgcggcg cgcgccgagg gtcggctcgg
     1081 tgctgcgcgg cggcagcgtc tcggcgtact cgccgacgag cttcggcgcg atgcggcgga
     1141 tggcgttcgt caaatcctga tcgccgctgc tgatgacggc gcgcgcggcc tcggtcagcg
     1201 tgcgctcgcg caggtctgcg gggacgctgc cctcgtctgc gacgccgaag agcacgacgc
     1261 cgcgcgccgt ctcaaggtcg agccggacgg ccttgtcctg ctccggcttc ttcgcgccga
     1321 agcgccccga gatttcgaac gtgccgccgc gcttctccct gaccttctcc agcgcgacct
     1381 cgctgtagta gcgcgggcgc gcgagcgtcc acttgaagtt gtagcgcagg gcgtccaaag
     1441 gcacgtcggc gcggatggtg ccggtgcccg tcttgacctc ggcctcggcg acgttgccga
     1501 cgacatcgac cgcgcccgcc tcggtctcga ccacgacgcg cgagcgcacg gggatgcgca
     1561 ccgtcacgtc gatgcgctcg cgctcggcgc gcgcggggct gacagagagc ttcctgccgt
     1621 cgccgtccgg cttcgacgcg cccgcgcgtt cgacctcgat gacgacgcgg cccccgcccg
     1681 actcgacgcg cacgtccttc tcgctgatcg cagctcccgc cgagacggcg cgcacggaca
     1741 ccttcttctg ctgctcctcg gcggcgatca ccgtcacccg accgagccgg ttctgcacgc
     1801 gcagctcgac ggtctcgccc gcctctatct cgcgctcgta gccttgcgcg cgcgccggcg
     1861 gggccgcgca cgcgagcacg acggccaacg ccgtcaggaa gcagaacgcg cgccccgaac
     1921 cgtagacgcg ccccgcgggg gtgtgacgaa acacaggcat ggtaagactc cgaaaaagct
     1981 ttaggggaaa aggcgaaaag gggaaggcgg aagaggtttc gcttttcctt ttcccccttc
     2041 cgtcatttcc tcttgtccag gtagaaactg aaggcgtcgc gcaggcgct
//
LOCUS       DBNGOH010000553         2783 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1731452, whole genome shotgun sequence.
ACCESSION   DBNGOH010000553 DBNGOH010000000
VERSION     DBNGOH010000553.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2783)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2783)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2783
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1731452"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(86..1144)
                     /locus_tag="AC159P_10745"
     CDS             complement(86..1144)
                     /locus_tag="AC159P_10745"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF014244.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HIR3695969.1"
                     /translation="MSESIRSIPAFASGEEVAAGSRRRRGGAARPTRREWVRHTTLFL
                     LTLLTATWAGTMSAVMGMADAPQPFVAAPSTPLDYVLFVPLELFQQLAADASYALAHP
                     QLFFDGAVFALALLTILLAHEAGHYVACRLYGVDATLPFFIPMPPPFMAGTLGAFIKI
                     RSPIPSRRALFDIGVAGPLAGFVALVPVAAAGLLLSTPQTLPPDAPFLIRFNDPLFMR
                     LLAWGLGVENLSHIAPNPFYFAARIGLLVTSLNLMPVGQLDGGHAVYALFGARAHALV
                     GRAAFLFVVLLAPLGWLLHGAPTGIVYALLLFIMLRLRHPRAEDESDPPGRARTLVAL
                     ATLVVFLLSFLPFPLTVL"
     gene            complement(1394..1648)
                     /locus_tag="AC159P_10750"
                     /pseudo
     CDS             complement(1394..1648)
                     /locus_tag="AC159P_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014267048.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
     gene            2004..2684
                     /gene="lepB"
                     /locus_tag="AC159P_10755"
     CDS             2004..2684
                     /gene="lepB"
                     /locus_tag="AC159P_10755"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HIR3695970.1"
                     /translation="MFGRKRRGEPPFGDIAVTDDRDIEVRRLGDESRRHFWAEARLLL
                     RDLVFALLVIALVLVFIVQPVKVEGTSMLPRLHDGERIFVNKLVYYDIPELERGDIVV
                     FWFPDDPTKSYIKRVIGLPGEIVHVRDGRVFVNGRELQEPYLDPGRNVTRGTEQPVLV
                     KQHYFFVMGDNRDNSSDSRAWGLVPEKYIYGKALFRYWPLSEASVIRHETEYPDIPRR
                     VPIIESDE"
BASE COUNT          501 a          869 c          960 g          453 t
ORIGIN      
        1 tcaagcctct tgctgttctg ttttctgact tctgactcct gtcttctgtt tttaactgct
       61 cactgctcac tgctcaccgc tcactctaaa ggacggtcag cgggaagggg aggaacgaga
      121 gcaggaagac gacgagcgtg gcgagcgcga cgagcgtgcg cgcgcgtccg ggcgggtcgc
      181 tttcgtcctc ggcgcgcggg tggcgcaggc gcaacatgat gaagagcagc agcgcgtaga
      241 cgatgccggt cggcgcgccg tggaggagcc agccgagcgg ggcgagcagg acgacgaaga
      301 gaaaggcggc gcgcccgacc agcgcgtgcg cgcgcgcgcc gaagagcgcg tagacggcgt
      361 ggccgccgtc gagctgcccg accggcatca ggttcaggct cgtcaccagc agcccgatcc
      421 gcgccgcgaa gtagaaaggg ttcggcgcga tgtgcgacag gttctcgacg cccaaccccc
      481 acgcgagcag ccgcatgaag agcgggtcgt tgaagcgtat gaggaagggc gcgtcgggcg
      541 ggagcgtctg cggcgtgctc aagagcagac cggcggcggc aacggggacg agcgcgacga
      601 agcccgcgag aggccccgcc acgccgatgt cgaagagcgc gcggcgcgag gggatgggcg
      661 acctgatctt gatgaacgcg ccgagcgtgc ccgccatgaa gggcggcggc atcgggatga
      721 agaacgggag cgtcgcgtcc acgccgtaga ggcggcaggc gacgtagtgg cccgcctcgt
      781 gcgcgagcag gatcgtcagg agcgcgagcg cgaagaccgc gccgtcgaag aagagctgcg
      841 ggtgcgcgag ggcgtaagag gcgtccgccg ccaactgctg aaaaagctca agcgggacga
      901 agaggacgta gtcgagcggc gtcgagggcg cggcgacgaa aggctgcggc gcgtccgcca
      961 ttcccatgac cgcggacatc gtgcccgccc acgtcgccgt cagcagcgtc agcaggaaga
     1021 gcgtcgtgtg ccgcacccac tcgcgccgcg tcgggcgcgc ggcgccgccc cggcgtcggc
     1081 ggctgccggc ggccacctcc tcccccgaag cgaaagcggg gatgctgcgg atcgattcag
     1141 acatatgaaa ggccgtgaac ggtaaaccgt aaaccgtgac gagaagaaat ccgttcttca
     1201 cttgtcacgg tttgcggttt accgttcacg gctcttagta ttccgtgcgc ggctcggtgt
     1261 tgcccgcgtc attgtcggcg gcgggggcgg cggcggtgtc gtcgggcgtg gggcctccgg
     1321 tgtggaggtc ttcgtggtgc tcgtcgttgt cgtggtggtc ttcgtcgtgc tcgccggaga
     1381 agccgtgcgc ctgaagctcc ttgccgggct tgaagcggat ggtcttgccg gaggggatgg
     1441 caacctcttc gccggtgcgc gggttgcggc ccacgccgcg cttgcgcggc ttgacgacga
     1501 agacgccgaa gccgcgcaac tcgatgcgct cgccgcgcgc cagcgcctcc ttcatcgagt
     1561 ggaagagggc gtccacggcc tgctcggcct tcatcttcgg gacgcccgtc cgctcggcca
     1621 cacggttcac gatgtcgagt ttaatcacgt cgcttccaaa gcctctcttt cggcctcgcc
     1681 accttccgcg agagtgtggc ttagtctatg ggtggcaaca acttctggcc gataatagag
     1741 acgcgttgcc gaagctgtca agcatcggaa cagtaggccg taggcagtag gcagtaggca
     1801 gcgcggtgtt tcggacgaaa gatacgtgct catcctgacc atccggctgc aaaagatttt
     1861 aactgcttac tgcctactgc ctacggccta ctgatgtcgc gttgacaaag ctcgtgcggc
     1921 gcggctaacc tcgaaaatcg ggtcgggctt cggcgaggcg catccgcggg aggggcgggc
     1981 gcatcgaatc ggtcgagatg ctcatgtttg gacgcaagcg gcgcggcgag ccgccgttcg
     2041 gcgacatcgc cgtgacggac gaccgcgaca tcgaagtgcg gcgcctcggc gacgagtcgc
     2101 gccggcactt ctgggccgag gcgcggctgc tgctgcgcga tctggtcttc gcgctgctgg
     2161 tcatcgcgct cgtgctcgtc ttcatcgtcc agcccgtcaa ggtcgagggc acgtcgatgc
     2221 tcccgcgcct gcacgacggc gagcgcatct tcgtcaacaa gctcgtctac tacgacatcc
     2281 ccgagctgga gcgcggcgac atcgtcgtct tctggtttcc ggacgacccc acgaagagct
     2341 acatcaagcg cgtcatcggg ctgcccggcg agatcgtgca cgtccgcgac gggcgcgtct
     2401 tcgtcaacgg cagggagcta caggagccgt acctcgatcc gggccgcaac gtcacgcgcg
     2461 gcaccgagca gcccgtgctc gtcaagcagc actacttctt cgtgatgggc gacaaccgcg
     2521 acaactcttc ggactcgcgc gcgtggggac tggtgcccga aaaatacatc tacggcaagg
     2581 cgctcttccg ctactggccg ctctccgagg cgagcgtcat ccgccacgag accgaatacc
     2641 ccgacatccc ccgccgcgtc cccatcatag aaagtgacga atagcaggca gcgcgcagca
     2701 ggcagcagga agtgaaaacc gatggagtga tacgggtctt gagtcacatc ccagaaagtt
     2761 caaacatgaa agtgtcaata act
//
LOCUS       DBNGOH010000554         2620 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2830856, whole genome shotgun sequence.
ACCESSION   DBNGOH010000554 DBNGOH010000000
VERSION     DBNGOH010000554.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2620)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2620)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2620
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2830856"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..645)
                     /locus_tag="AC159P_10760"
     CDS             complement(<1..645)
                     /locus_tag="AC159P_10760"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3695971.1"
                     /translation="MRTKTSFTAVAASLVLLCAAAVASAQSGATRPRRVVPVQPTPDP
                     AATSRAATPAPVTRAGGAGVTGAAAQGTTQHAFALLQQKQYDAALKEAKQIAAADPKN
                     AEAWKIAGFAELELKQYAEAAADLKRALELQRAAGSPDPNTSDALIRAYMRAEQFDAA
                     LPLLVEATTRKGAAPDANLLYSRGLAEFKTNKPADAERSFAAAAKADADYLAAVR"
     gene            941..1504
                     /locus_tag="AC159P_10765"
     CDS             941..1504
                     /locus_tag="AC159P_10765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012826736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR3695972.1"
                     /translation="MPATHELTEMLQAWCEGDGEALERLTPVVHEELRRLARRYMSRE
                     RPGHTLQTTALVNEAYMRLIDWKNVRWQNRAHFFAVSAQLMRRILVDFARRRPRAEGG
                     EVRHVSLDEAMTVSHGRDPDLVALDEALDALAALDPRKSRVVELRFFGGLSVEETAEV
                     LRVAPVTVMREWNKAKLWLHREISRQP"
     gene            1533..>2620
                     /locus_tag="AC159P_10770"
     CDS             1533..>2620
                     /locus_tag="AC159P_10770"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HIR3695973.1"
                     /translation="MDELLDAALELPAAEREDYLAAACGDDEALRREVSSLLGEEQKV
                     AGGFMEHSAMKVAAQALAQQTVRLNVSPVGREVGPYRIERLIGAGGMGEVYLAQNLRL
                     SRKAALKILPAEFIADPERVLRFEREARAVSALNHPNIVTVYDVGESEGWHYIATEFV
                     EGRTLRDLAGDGLKLKEMLSYAAQAAEALSAAHGAGVTHRDIKPENIMVRADGYVKVL
                     DFGLAKLAEPRGAAGAGDARPPVETRAGVVMGTLAYMSPEQAAGDVVDSRTDIWSLGV
                     VLYEMAVGKTPFASGTRQATINAILSSEPEAAGASLPELPAEFDHVLAKALEKDRELR
                     YQTASDFRADLRRLLREIDSSASWSSDKV"
BASE COUNT          390 a          866 c          971 g          393 t
ORIGIN      
        1 gcgcaccgcg gcgaggtagt cggcgtcggc cttcgcggcg gcggcgaagg agcgctcggc
       61 gtccgcgggc ttgttcgtct tgaactcggc gaggccgcgc gagtagagca ggttcgcgtc
      121 cggcgcggcg cctttgcgcg tcgtcgcctc gacgaggagc gggagcgccg cgtcgaactg
      181 ctcggcgcgc atgtaggcgc ggatgagggc gtcgctggtg ttcgggtcgg gcgagcctgc
      241 ggcgcgctgt agttcgagcg cgcgcttgag gtcggcggcg gcctctgcgt actgcttcag
      301 ctccagctcg gcgaagccgg cgatcttcca cgcctccgcg ttcttcgggt cggcggcggc
      361 gatctgcttg gcctccttga gcgcggcgtc gtactgcttc tgctgaagga gcgcgaaggc
      421 gtgctgcgtg gtgccttgcg cggcggcgcc ggtcacgccc gcgcccccgg ctcgcgtgac
      481 gggcgcgggg gtggcggcgc ggctcgtcgc cgcggggtcg ggcgtgggct gcacggggac
      541 gacgcggcgc gggcgcgtgg cgcccgactg cgcggaggcc acggcggcgg cgcacaacag
      601 cacgagcgag gcggcgacgg cggtaaagct ggtcttcgtt ctcatcatgt cggaaagttt
      661 ttcacagcct gaagggttat tcaactcgca aggagcctcg gtttcggcgg gagagtggcc
      721 acccttcgga tgccgcggtg cggattcggg ttcgccggaa cgggcggagc gggaaagcgg
      781 ggaaaaagag gaaaagggga agaggggaaa aggggaaggc cctgtcgcct ctcccccttt
      841 catcttttcg ctattaccct cttcgcctct tccccttcgg gccgtctccg aaggtgtgct
      901 acattaccgg ccaacggaac ctgacccttc cgtacgaacc atgcccgcga cgcatgaact
      961 aacggagatg ctccaagcgt ggtgcgaagg ggacggcgaa gctttggagc ggctgacgcc
     1021 ggtcgtccac gaagagctgc gccgcctcgc gcgccgctac atgagccgcg agcgaccggg
     1081 ccacacgctg caaaccaccg ccctcgtcaa cgaagcctac atgcggctca tcgactggaa
     1141 gaatgtccgc tggcagaacc gcgcgcactt cttcgccgtc tccgcgcagc tcatgcgccg
     1201 catcctcgtg gacttcgcgc gccgccgccc gcgcgccgaa ggcggcgagg tgcgccacgt
     1261 ctcgctcgat gaagcgatga ccgtctcgca cgggcgcgac cccgacctcg tcgcgctgga
     1321 cgaggcgctc gacgcgctcg cggcgctcga cccgcgcaag agccgcgtcg tcgagctgcg
     1381 cttcttcggc ggcctctcgg tcgaggagac ggcggaggtc ttgcgggtcg cgcccgtcac
     1441 cgtcatgcgc gagtggaaca aggccaagct ctggctccac cgcgagatca gccgccaacc
     1501 ctgaaagagt ggaacccgaa cgctggaaac gtgtggacga gctgcttgac gccgcgttgg
     1561 agttgcccgc ggcggagcgc gaggactatc tcgcggcggc gtgcggcgac gacgaggcgc
     1621 tgcgccgcga ggtgtcgagt ctgctcggcg aggagcagaa ggtcgcgggc ggcttcatgg
     1681 agcactcggc gatgaaggtc gcggcgcagg cgctcgcgca gcagacggtg cgcctcaacg
     1741 tctcgcccgt gggccgcgag gtcggcccct acaggatcga gcgcctgatc ggcgcgggcg
     1801 gcatgggcga ggtctacctc gcgcaaaacc tccggctcag ccgcaaggcc gcgctgaaga
     1861 tactgcccgc cgagttcatc gccgacccgg agcgcgtgct gcgcttcgag cgcgaggcgc
     1921 gcgccgtctc ggcgctgaac cacccgaaca tcgtcaccgt ctacgacgtg ggcgagagcg
     1981 agggctggca ctacatcgcc accgagttcg tcgagggccg caccctgcgc gacctcgcgg
     2041 gcgacgggct gaagctcaag gagatgctct cctacgcggc gcaggcggcg gaggctcttt
     2101 cggcggcgca cggcgcgggc gtcacgcacc gcgacatcaa gcccgagaac atcatggtgc
     2161 gcgccgacgg ctacgtgaag gtgttggact tcggcctggc gaagctcgcg gagccgcgcg
     2221 gagccgcggg cgccggggac gcccgcccgc cggtcgagac gcgcgcgggc gtcgtgatgg
     2281 ggacgctcgc ctacatgtcg ccggagcagg cggcgggcga cgtggtggac agccgcacgg
     2341 acatctggtc gctgggggtc gtgctgtacg agatggccgt cggcaaaact ccgttcgctt
     2401 cgggcacccg gcaggcgacg atcaacgcca tcctgtcgag cgagccggag gccgcgggcg
     2461 cgtccctgcc ggagctgccc gcggagttcg accacgtgct agcgaaggct ctggagaaag
     2521 accgcgagct gcgctaccag acggcctcgg acttccgcgc cgacctccgg cggttgttgc
     2581 gcgagataga ctcttcggct tcttggtcga gcgacaaggt
//
LOCUS       DBNGOH010000555         2277 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3931874, whole genome shotgun sequence.
ACCESSION   DBNGOH010000555 DBNGOH010000000
VERSION     DBNGOH010000555.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2277)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2277)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2277
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3931874"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2123
                     /locus_tag="AC159P_10775"
     CDS             <1..2123
                     /locus_tag="AC159P_10775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695974.1"
                     /translation="TAFARDVFEGGSFTFARLSHGELLPGSETVAIEVRDRRNPERIL
                     SRESLLRGIDYNLDPVTGEIFFLRPISTFDFQLNLVQVVATYEYRAAGMSSAVYTGRA
                     FKTFERLGLKLGLSFVDQRQGEFGSFVVGGIDAEKTLPRRGRLALQWATSRGRAMVGG
                     NIFGGGTGDERHDGNAYRAELEQPLPFREATLRASFVRADEGFLNPFGQTITPGSQRA
                     EGSIEMKATKSSVVRFGLMDERNRTANVGNSRQTGSLLWTQVFGDKLRTTFGYDFRRF
                     RDELSGTGTDSNLLTVGAEWQATDKLNVSVKREQNLTEADPTYPNQTTLAAKYQWNQF
                     TNIFFTQRLASAPIVPISDAAATGFASVGSQRETAIGVETKLGKYANLVSRYQLENGI
                     NGTDSFAVIGLSNRFDLTKQLSLDLGYERGFHLAGGGESFNSGHFGFLWNPTEDFRAN
                     ARYELRDRGGLGTILSFGAAGRLGDGLTSLGRFQLSRVRFGGRDNTGLNATAAVAWRP
                     LESDRAGLLFSYTRRDFRQGAFETTAETRDRADVLSSDGYYQPTRDIELYGRFALKFG
                     DTASQELARVSTLTYMTQGRFVYHFGRQFDAAGEARLLMQPSSATRRNSFGAELGYWV
                     LPDLRLGGGYNWTGGAEPLHGAFVNGRRGFYFTISSKLSNLFDLFGTSRTNAAAAEGA
                     GGDASAKQGKKKDDDEEEDEDEEE"
BASE COUNT          370 a          842 c          721 g          344 t
ORIGIN      
        1 acacggcgtt cgcgcgcgac gtgttcgagg gcggctcgtt caccttcgcg cgcctctcgc
       61 acggggaact cctgcccggc tccgaaacgg tcgccatcga agtgcgcgac cgccgcaacc
      121 ccgagcgtat cctctcgcgc gagtcgctgc tgcgcggcat cgactacaac ctcgaccccg
      181 tgacgggcga gatattcttc ctgcgcccca tctcgacctt cgacttccag ctcaacctcg
      241 tgcaggtcgt ggcgacttac gagtaccgcg ccgccgggat gtcttcggcg gtctacacgg
      301 gccgcgcctt caagacgttc gagcggctcg gcctcaagct cggcctctcg ttcgtcgatc
      361 agcggcaggg cgagttcggc tcgttcgtcg tcggcggcat cgacgccgag aagacgctgc
      421 cgcgccgcgg gcgtctcgcc ttgcagtggg cgacctcgcg ggggcgcgcg atggtcggcg
      481 gcaacatctt cggcggcggc accggcgacg agcgccacga cggcaacgcc taccgcgccg
      541 aactcgaaca gcccttgccc ttccgcgagg ccaccctgcg cgccagcttc gtccgcgccg
      601 acgaaggctt cctgaacccc ttcggccaga ccatcactcc cggctcgcag cgcgccgaag
      661 gctcgataga gatgaaggcg acgaagtctt ccgtcgtccg cttcggcctg atggacgagc
      721 gcaaccggac ggcgaacgtc ggaaacagcc gccagaccgg ctcgctcctg tggacgcaag
      781 tcttcggcga caagctgcgg acgaccttcg gctacgactt ccgccgcttc cgcgacgagc
      841 tgtcggggac gggcaccgac tcgaacctgc tgaccgtcgg cgccgagtgg caggccacag
      901 acaagctcaa cgtctccgtc aagcgcgagc agaacctcac ggaggccgac ccgacctacc
      961 cgaaccagac gacgctcgcc gccaagtacc agtggaacca gttcaccaac atcttcttca
     1021 cgcagcgcct cgcctcggcg ccgatagtcc ccatcagcga tgcggcggcc acgggcttcg
     1081 cctcggtcgg ctcgcagcgc gagacggcca tcggcgtcga gaccaagctc ggcaaatacg
     1141 ccaacctcgt ctcgcgctac cagcttgaga acggcatcaa cggcaccgac agcttcgccg
     1201 tcatcggtct gtcgaaccgc ttcgacctga ccaaacagct ctcgctcgac ctcggctacg
     1261 agcgcggctt ccacctcgcc ggaggcggcg agagcttcaa cagcggccac ttcggtttcc
     1321 tgtggaaccc gaccgaagac ttccgcgcca acgcgcgcta cgagttgcgc gaccgcggcg
     1381 gcttgggcac aatcctctcg ttcggcgccg cgggccgcct cggcgacggg ctgacttcgc
     1441 tcgggcgctt ccaactctcg cgcgtgcgct tcggcgggcg cgacaacacg ggcctcaacg
     1501 cgaccgctgc cgtcgcgtgg cgcccgctcg aatccgaccg cgccggtctt ctcttcagct
     1561 acacgcgccg cgacttccgg cagggcgcct tcgaaacgac tgccgagacg cgcgaccgcg
     1621 ccgacgtgct ctcttcggac ggctactacc agccgacccg cgacatcgaa ctctacggtc
     1681 gcttcgcgct caagttcggc gacacggcct cgcaggagct ggcgcgcgtt tcgacgctca
     1741 cctacatgac gcaggggcgc ttcgtctacc acttcggcag acagttcgac gccgccggcg
     1801 aggcgcggct cctcatgcag ccctcttcgg ccacgcgccg caactcgttc ggcgccgaac
     1861 tcggctactg ggtgctgccc gacctccggc tcggcggcgg ctacaactgg acgggcggcg
     1921 ccgagccgct gcacggcgct ttcgtcaatg ggcggcgcgg cttctacttc accatctctt
     1981 ccaagctctc gaacctcttc gacctcttcg gcacctcacg cacgaacgcg gcggctgcgg
     2041 aaggcgcagg cggcgacgcg tcggcaaagc aggggaagaa gaaggacgac gacgaagaag
     2101 aggatgagga cgaggaggaa taggcttgtc gcgtgcggca caggcggcgg agaggagaga
     2161 ggcgcgggga ggcgcgaggc gcgacgggcg acgcggccac gcggcctttc gcacgctcgc
     2221 ccggcgcgcc ctcgcctgcg tcttcgtgct cgccgcgtgg cagaccgcgc acgcttc
//
LOCUS       DBNGOH010000556         5016 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4663306, whole genome shotgun sequence.
ACCESSION   DBNGOH010000556 DBNGOH010000000
VERSION     DBNGOH010000556.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5016)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5016)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5016
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4663306"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..641)
                     /locus_tag="AC159P_10780"
     CDS             complement(<1..641)
                     /locus_tag="AC159P_10780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HIR3695975.1"
                     /translation="MDWLMSEKVPLAGIAVISKTNLEHPQVIADYFIRRGIPFRVLDI
                     DQATDTLPQTRQAAASFAQYQEFFKNLHRLDAVQQALREGLCIDPLSTARAMLEQWRR
                     GYRSPLSGDAHGHQEWALAVNTNGDVYSPGDCYVRKFEYGSIFSQPLDEILFLSEGRR
                     RRIARSKKRCQSICRHCFLYGKGCEGVYVSHATPERVRNYRRHKGRHYGLLAG"
     gene            complement(1034..3016)
                     /locus_tag="AC159P_10785"
     CDS             complement(1034..3016)
                     /locus_tag="AC159P_10785"
                     /inference="COORDINATES: protein motif:HMM:NF012698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HIR3695976.1"
                     /translation="MSSLKETAPTCYRFDDVVVDCRNFRVVKGEQKRDLTPRAFDVLR
                     YLIERPDRVVEKQELFERVWKEKFVSDNALTRTVKEIRQALGDSADAPRYIETVPKRG
                     YRFIAPMSPQGRVASGADVSEDGRPGVVETRGAETAGGGGVAPAAGAQPLRGAAGRLK
                     PALVVLVVAAALALVAAVLYSRRSRPAAGEAVGAPVRSIAVLPLKNLSGDAVNEYFSD
                     GMTESLINALSKIEGLKVISRSSAFNFKGRDVDPREVGRRLGVAAVLEGSVRKDADSV
                     RVAARLVSAEDGRVLWASDAQDHALGDIFALQDEIARGMTAALKLKLTGEGERLLARR
                     YTDDLEAYDLYLKGRFFWNKRTDAGLRKSIEHFERAIARDPDYALAYAGLADSYAVFN
                     LYSPAQLKDASPRAKEAAAHALALDDTLAEAHATLGLLKQQYDFDWPGAEREYRRAIE
                     LDPNYATAYQYYAEHLALLGRTEESVAQIRRARDLDPLSLIINTEMGFPYLCARRWDE
                     ALEHYRHALEMDPSFHLAIYFSARAHAQQGRLDEAVAESRRAVALSGGSTLTVGGLGY
                     VYAAAGQSEEARKVLGELTALSRRRYVSPYTFATVHVGLGERDLALARLEEAYRERDY
                     QLVMLRIDPRLDSLRDDPRFTDLMRRVGLAPESSSD"
     gene            3238..4611
                     /locus_tag="AC159P_10790"
     CDS             3238..4611
                     /locus_tag="AC159P_10790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695977.1"
                     /translation="MTFGKFFLALSILALSCAHEAAAQGYEVWMTDQNNTAGFGAAAP
                     RGTHGGRLLIYEGSDLDAPGGPANSALVIDFAALYAVGGPHNATGANVVRPHMAVPSP
                     DHRFVAVAFVASGHVAILDGASKQSRALFRMSAGAGGARQAHAGFWLPDGSALVVANQ
                     NGKLLERIRYDAAADTFTHETAATLDLVACTTPSGHPCQSNTPVSDQDPAYAGPHNRP
                     DNAPICPVVSANRSAFVTLRGGGLFVVDPTASPMAIVAAYGNQFVGRDGCGGVQDKKN
                     VYLNGGTGTLLTNPEEFSLYHFRDKYPTAPAALADNDPDSLPDVFYRDPSPHRDAHGI
                     VLTIGQDYLWQFDRLANVAEVFRLPSKQHIGTVDLAASGVSTDPTPDLGAASPRGDRI
                     YVALRGPQPQTGAHAAFGLTPGLGVVTLTGGGASGSLSHLFPTSFINPFNGSQESDPH
                     AAFVRLK"
     gene            4662..>5016
                     /locus_tag="AC159P_10795"
     CDS             4662..>5016
                     /locus_tag="AC159P_10795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695978.1"
                     /translation="MNKRSRSTHHANGARPAPAKRRAMLAVLVTLALLLPPAALARLV
                     FDRDDPAFIGAILVSFAPGDAAQGATSFTLTRNGVEFTFSTTGSQGIFFCSGGNCFLQ
                     APFPQGIDVQINPPVA"
BASE COUNT          807 a         1787 c         1609 g          813 t
ORIGIN      
        1 caacccgcca gcagcccgta atgacggccc ttgtgacgac gataatttct cactctctcg
       61 ggcgtcgcgt gagagacata aacgccctcg catcccttcc cgtagagaaa acaatgacgg
      121 cagatggact ggcaacgctt tttggatcgc gcgatgcgcc ggcggcgtcc ctccgacagg
      181 aaaaggattt cgtccagcgg ttgcgagaag atgcttccgt attcgaactt ccggacgtag
      241 caatcgccgg gcgagtacac gtcgccgttg gtgttcaccg ccaaagccca ctcctgatgg
      301 ccgtgcgcgt cccccgaaag aggcgaacga tacccgcgcc gccactgttc gagcatcgcg
      361 cgggccgtcg agagcgggtc gatgcagaga ccctcccgga gcgcctgttg cactgcgtca
      421 agacggtgga ggtttttgaa gaactcttga tactgcgcga acgaggccgc ggcctgtcgc
      481 gtctgcggga gcgtgtcggt ggcctgatca atgtccagaa ccctgaacgg gatgcccctg
      541 cgaatgaaat aatccgcgat cacttgcggg tgctcaaggt tggttttgga gatgaccgcg
      601 attcccgcca ggggaacctt ctccgacatc agccagtcca cctttcgccg gacgacctcg
      661 gcggtagggc tgcctccggc atcgacgcgc aggtcgttga ttacgtcgaa ggagaacccc
      721 agctcgaaat ggcgcagcag cggaagatgg ttttctttca ggagcgtgag attcgattgg
      781 atggtgttgg tcagcgaacc gcgttgaaac tcttcgccgt agactttccg ctgcgcggcg
      841 agaatgcttt cccagtagtc aatgggttgt gcgaacggct ctccgccatg ccagatgaat
      901 tccagcttgt gggtttccgc ctcggccccc cagtcgtgca gatactcgcg gatgtgtacg
      961 aagcggcgcg aacggcagaa acagcgcgcg ccctttcgtc tctgatctgt aggcgggcgg
     1021 acggtggcgg cgttcagtcc gaagagcttt cgggggcgag gccgacgcgg cgcatgaggt
     1081 ctgtgaagcg cgggtcgtcg cgcagcgagt cgaggcgtgg gtctatcctg agcatgacga
     1141 gctgatagtc gcgctcgcgg taagcctctt cgagccgcgc gagcgcgagg tcgcgctcgc
     1201 cgagtccgac gtgcaccgtg gcgaaggtgt agggcgagac atagcgccgc cgcgagagcg
     1261 ccgtcaactc gccgagcacc ttgcgcgcct cttcgctctg gcccgccgcg gcgtagacgt
     1321 agccgaggcc gccgaccgtc agcgtgctgc cgccggagag ggcgaccgcg cggcgcgact
     1381 ccgcgacggc ctcgtcgagc cggccctgct gcgcgtgggc gcgcgcgctg aagtagatgg
     1441 cgaggtggaa gctcgggtcc atctcaagcg cgtggcggta atgctccagc gcctcgtccc
     1501 agcgccgcgc gcagaggtag gggaagccca tctccgtgtt gatgatgagc gagagcgggt
     1561 cgaggtcgcg cgcgcggcgg atctgcgcca cgctctcctc cgtgcggccc agcagcgcga
     1621 ggtgttcggc gtaatactgg taggcggtcg cgtagttcgg gtcgagttcg atcgcccgcc
     1681 ggtattcacg ctcggcgccc ggccagtcga aatcgtactg ctgtttcaac agcccgagcg
     1741 tggcatgggc ttcggcgagc gtgtcgtcga gcgcgagcgc gtgcgcggcg gcctccttcg
     1801 cgcggggcga agcgtctttg agctgcgcgg ggctgtagag gttgaagacg gcgtaggagt
     1861 cggccagccc cgcgtaagcc agcgcgtaat ccgggtcgcg tgcaatcgcc cgctcgaagt
     1921 gctcgatgct tttcctgagg cccgcgtccg tccgcttgtt ccagaagaag cgccctttca
     1981 ggtacaggtc ataggcttcg aggtcgtccg tgtagcggcg cgcgagaagg cgctcgcctt
     2041 cgcccgtgag cttcagcttc aaggccgccg tcatgccgcg cgcgatctcg tcttgcagcg
     2101 cgaagatgtc gccgagcgcg tgatcctgcg cgtcgctcgc ccacagcacg cgcccgtcct
     2161 ccgcgctcac gagccgcgcc gccacgcgca ccgagtccgc gtccttccgc acgctcccct
     2221 ccaagaccgc cgccaccccg agccgcctgc cgacctcgcg cgggtcaacg tctctgcctt
     2281 tgaagttgaa ggccgagctg cgcgagatca ctttcagccc ctcgatcttc gagagcgcgt
     2341 tgatcaggct ctcggtcatc ccgtcgctga agtactcgtt caccgcgtcg cccgacaaat
     2401 tcttcaaagg cagcacggcg atcgagcgca caggcgcgcc gacggcctcg cccgccgcgg
     2461 gccgcgacct ccggctgtag agaaccgccg cgacgagcgc gagggcggcg gcgacgacga
     2521 gcacgaccaa cgccggtttg agtcgtcccg ccgcgccgcg caaaggctgc gcgcccgccg
     2581 ccggggccac accgccgccg cccgccgtct ctgctccccg cgtctccacg acgccgggcc
     2641 gtccgtcctc ggacacgtcc gcgccgctcg ccactcgccc ctgcggactc atcggcgcga
     2701 tgaaacggta gccgcgcttg ggcacggtct cgatgtagcg cggggcgtcg gcgctgtcgc
     2761 cgagcgcctg acgtatctct ttgaccgtgc gcgtgagggc gttgtcggag acgaacttct
     2821 ccttccagac ccgctcgaac agctcctgct tttcgaccac gcggtcgggc cgctcgatga
     2881 ggtagcgcag cacgtcgaag gccctcggcg tgaggtcgcg cttctgctcc cccttgacca
     2941 cgcgaaagtt ccggcagtca accaccacgt cgtcgaagcg gtagcaggtg ggggcggtct
     3001 ctttgaggga agacatggcc gttctcagcg gaaagtcgcg gcgcggcggc gcggaaaacc
     3061 gaaaagtcac ggtgcggtca gcgttctgtc acgaccggcg aagccgccgc cgattatatt
     3121 ccccgccgct gggcaggcaa agaagttcgg agtgaaaacc ggcgccgctt cgagacgtga
     3181 ccttccgctg ccgggccgtc attactgagc gccaagccca aacaaaagga gactcatatg
     3241 acattcggaa aattttttct ggccctgagt atcctcgcgc tctcgtgcgc gcacgaggcc
     3301 gcggcccaag ggtacgaggt ctggatgacc gaccagaaca acactgccgg cttcggcgcc
     3361 gccgccccgc gcggcacgca cggcgggcgg ctgctgattt acgaaggcag cgacctcgac
     3421 gcgccgggcg gccctgcgaa cagcgccctc gtcatcgact tcgccgcgct gtacgccgtc
     3481 ggcggcccgc acaacgcgac gggcgcgaac gtcgtccgcc cgcacatggc cgtccccagc
     3541 cccgaccacc gctttgtcgc cgtcgcgttc gtcgccagcg gccacgtcgc catcctcgac
     3601 ggcgcgtcga agcagtcgcg cgcgctcttc cgcatgagcg ccggcgcggg cggcgcgcgg
     3661 caggcgcacg cgggcttctg gctgccggac ggcagcgcgc tcgtcgtcgc caaccagaac
     3721 ggcaagctgc tcgaacgcat ccgctacgac gccgccgccg acaccttcac gcacgagacg
     3781 gcggccacgc tcgacctcgt cgcctgcacg acgccctccg gccacccctg ccagtcgaac
     3841 acgcccgtga gcgaccagga cccggcctac gcggggccgc acaaccggcc cgacaacgcg
     3901 ccgatctgtc cggtcgtgag cgcgaaccgc agcgccttcg tcacgctgcg cggcggcggc
     3961 ctcttcgtcg ttgacccgac ggcttcgccg atggcgattg tcgcggccta cggcaatcag
     4021 ttcgtcgggc gcgacggctg cggcggcgtg caggacaaga agaacgtcta cctcaacggc
     4081 gggacgggga cgctcttgac gaaccccgaa gagttcagcc tctaccactt ccgcgacaaa
     4141 tacccgaccg cgcccgccgc gctcgccgac aacgaccccg actcgctgcc cgacgtgttt
     4201 taccgcgacc cctcgccgca ccgcgacgcg cacggcatcg tcctcacaat cggccaagac
     4261 tacctctggc agttcgaccg gctggccaac gtcgccgaag tcttccgcct gccgagcaag
     4321 cagcacatcg gcacggttga cctcgccgcc tccggcgtct ccacagaccc gacgcccgac
     4381 ctcggcgcgg ccagcccccg cggcgaccgt atctacgtcg ccctgcgcgg cccgcagccg
     4441 cagaccggcg cgcacgccgc gttcggcctc acgccgggac tcggcgtcgt gacgctcacc
     4501 ggcggcggcg cgtcgggcag cctctcgcac ctcttcccga cgagcttcat caaccccttc
     4561 aacggcagtc aggagagcga cccccacgcc gccttcgtcc gcctgaagtg acgcgcccgc
     4621 gcgtcacggt tttcttctcc aagcccaaag gagtttcatc catgaacaag agatcacgct
     4681 ccacccacca cgcaaacgga gcgcgaccgg cgcccgccaa acgccgcgcg atgctggccg
     4741 tgctcgtcac gctggcgctg ctgctcccac cggcggcgct cgcgcggctc gtcttcgacc
     4801 gcgacgaccc ggccttcatc ggcgcgatcc tcgtgtcttt cgcgcccggc gacgcggcgc
     4861 agggggcgac gagcttcacc ctcacgcgca acggcgtcga gttcactttc agcacgaccg
     4921 gctcgcaggg aatcttcttc tgctccggcg gcaactgctt tctgcaagcg cccttcccgc
     4981 agggcatcga cgtgcagatc aacccgccgg tcgccg
//
LOCUS       DBNGOH010000557        11613 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1548510, whole genome shotgun sequence.
ACCESSION   DBNGOH010000557 DBNGOH010000000
VERSION     DBNGOH010000557.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 11613)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11613)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11613
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1548510"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..399
                     /locus_tag="AC159P_10800"
     CDS             <1..399
                     /locus_tag="AC159P_10800"
                     /inference="COORDINATES: protein motif:HMM:NF017482.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT RNA binding domain-containing protein"
                     /protein_id="HIR3695979.1"
                     /translation="APRGGAGAIPGVRRYRSSDGLDILVGRGSRDNDHLTFRVARSQD
                     TWLHAADYSGSHVVVRNHRRDADVPHRTVIEAAQLAAHFSQARKDSKVAVNYTQRKFV
                     SKPKAAAPGLVRLSSFRTLLVEPREELERL"
     gene            654..1838
                     /locus_tag="AC159P_10805"
     CDS             654..1838
                     /locus_tag="AC159P_10805"
                     /inference="COORDINATES: protein motif:HMM:NF018805.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HIR3695980.1"
                     /translation="MSTNIIESFGKRAASVLDDIADAVVGKASTLRDGTEHTGVELPR
                     DLYAHDGTQTEWWYYTGHLQTQTGRRFGFELVFFKRRTDLDRFGVVPLRLIANPLYLA
                     HFAVTDESRGSFRYEHRKSANGPLDPAARTSAKHYYLRLGNWTVREAHGLHLLRATLG
                     DDLVFEAALKPTKAPVLNGDAGIGVSYKDRGEASRYFSFTRLEAEGDITWHGRTEHFK
                     GAAWMDREFGTWKTTEDQKGWDWFSVQLDDGAEVMVYHIRDRAGAPSAFSSGTYVDAA
                     GRWTHLTRADFDLDTTERWRSPHTGADYPSGWRLRVPRFGIDLEVKPVIKDQELDTRG
                     TTMIVYWEGACTARGTHGGHKTAGRAYVELVGYDRSHEQPSLSTFIFGGALDRRWRSI
                     FG"
     gene            2097..3176
                     /locus_tag="AC159P_10810"
     CDS             2097..3176
                     /locus_tag="AC159P_10810"
                     /inference="COORDINATES: protein motif:HMM:NF013775.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HIR3695981.1"
                     /translation="MMLPEVITSVEQVTPEWLTETLRRNGFLERGRVKDVSVEVTRTM
                     TVSIVSRLVASYAPGAAPHAPAHLFLKLSNPDFQPDAPCDRPPAEIVFYTSVVPEMPD
                     PPVVRCYDSAYSRSTGRSHLLLEDLSETHQQPSPHLPPTVAESEREVDALAAVHAHWW
                     EHPRLGKDIGRLLGASDAEEIARRNASDFARFADFLGDRLSPARRSLYERAFSVFPKP
                     WQRLTKAKGLTLTHGDAHFGNFLHPLDAEGGRTYLVDWQLWHPHIGPRDLAYMMTLFW
                     YPERRARMEETLLRRYHRGLAAGGVGDYTWEECFEDYRWSALRNIFIPILQWTRGQPG
                     WLWWSQLEKSLAAFEDLRCIELVEG"
     gene            3185..4504
                     /gene="hemL"
                     /locus_tag="AC159P_10815"
     CDS             3185..4504
                     /gene="hemL"
                     /locus_tag="AC159P_10815"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HIR3695982.1"
                     /translation="MTATRDDKQDTSARLFARAVELMPGGVNSPVRAFRGVGGNPRFI
                     KSASGATMTDVDGRTYVDYVGSWGPMILGHADAEVVEALREALVRGTSYGAPTELEIE
                     MAEEIARAVPSVEMVRMVNSGTEATMSALRLARGVTGRDRILKFEGCYHGHGDSLLVK
                     AGSGVATLGLPDSPGVPGGVAANTITAPFNDEEALERIFAEHGETIAAVIVEPVVGNM
                     GCVAPRAGYLDRMRDLTRRHGALLIFDEVMTGFRVSRGGAQELYNVLPDITTLGKIIG
                     GGLPVGAYGASRDIMNQVAPAGPVYQAGTLSGNPLAMTAGLVTLRRLRDPAVYERLER
                     AGQTLCDQLAEAAREAGVATVTNRVGSMFTTFFTSEPVHDWTTAAKSDRGLYAQFFHA
                     MLDAGVYLAPSQFEAAFLGVAHTDELIERTIEAARKAFRALAAARGD"
     gene            complement(4597..6435)
                     /locus_tag="AC159P_10820"
     CDS             complement(4597..6435)
                     /locus_tag="AC159P_10820"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     NtrB"
                     /protein_id="HIR3695983.1"
                     /translation="MSEGEQQKPEHVVYGDAGEARAREERERRAASEAAGQGTLWQAL
                     LPLLVGFALLIALVIGLGVLSRRELEEVRFKTEQEERKLSEMLRRLLDLRLALSKLDT
                     EARIRGQVEAGTKGVMLPPSDLRLRNERGAVEKMLPLFASLPLKDEAKKARVRQRIVD
                     YIEITKDPGSYSINGYPAYRDLEAELKALSEEVGGESEAIRGRRDDELKGVQGEVRFL
                     QFLAAITGLVVAAATTFEVLRRFRQLRRGYDALRQERQFSAQMLEGMVSAIAAIDRED
                     RIRSANAPFYEVFPGARPGTSIHDDFTTAEGIKLLAAATSTRVERATYRGRWNMGRDK
                     RGDERAFDVYSSPLEIEGEPGQLLTMVDVTEASEAEQVLRRQEALAAVGQSAAQIAHE
                     IKNPLGSIRLGVAMLRDMARDRESINTIDLVERGIEHLSKLTLDVTQFSRTKRLTRSE
                     VDLHALLDGSLELIFDRLNEKHTPVEKQYTPEPLRGNWDDDQLRQVFVNLVTNAVDAG
                     GEGSPVRLTTETLTANAPRAPHGGEGRPAPFARVTIADRGAGMDERTRARIFEPFFTT
                     KRKGTGLGLAIAKQIVEQHGGRIDVHSQPGEGTRFTVDLPLSANGT"
     gene            complement(6589..9324)
                     /gene="polA"
                     /locus_tag="AC159P_10825"
     CDS             complement(6589..9324)
                     /gene="polA"
                     /locus_tag="AC159P_10825"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HIR3695984.1"
                     /translation="MPPKKATPQGPTERVFLIDSFSHIFRAFFAPMGARTEPLTNSQG
                     QVTQAVFVFTNMLRKLLSDEQPHYIAAVFESGEPTFRHEMNVGYKANRAAPPDELESQ
                     IPYIVRVCEAYNIPILNAPGFEADDVIGTLAVQAAEAGLQAVVVSNDKDMTQLVRDPW
                     VVCMRQNSQNVKRKEPVPPVEWCDAAWVEARFGVPPAQMVDLLGLMGDSVDNIPGAPG
                     IGAKGAVQIVKQFGSIENALKNWEEVKHKTYRESLRDNAELILQSKDLATIRTDVNVK
                     LDLEKLRSRPPDRAAAYKLFRELEFQTLTREFADAATEVDTGGVAKNYRQVRTVAELE
                     TLVRKLWDAEHWAFAVADGTPAGDGQQSSVREQSAPPAGLAISYAPHTAHFVNFAEFE
                     GGAGRAAAMLRDVLSNGLLSKSVHDLKRATALLETLGVEVEGVTDETLLAAYLLDPTR
                     SRYDLGDLAREAVGADGWTEPRGEEWGEASWRTAEAADFTAQVADVLHGRILEQGLES
                     IYSEIEIPLAPLIHRIERAGLRVDTSVLGELSALFGAELAKLTERIYKEAGREFKINS
                     PKQVGEVLQELNISTNRKTATGQVSTNRAVLEELAQQYELPRLIIEYRELDKLKATYT
                     DALPALVGADGRIHSELNQTVTATGRLSSSNPNLQNIPIRTELGRRIRRAFVPDEGNV
                     FVAADYSQLELRLLAHITQDAHMLEAFQKGEDIHERTARLVFKAKTKEELKEKRRFAK
                     IVNFAIAYAVEPFGLSQRVGISRKEAKQVIEDYYNTYTGVRRFMDEVPVRARELGYVR
                     SLYGRIRPIPAINDRNANLRFRAEREAINMPIQGTASDIVKIAMLKVDEALRNENLKA
                     RMVMQVHDELLIEAPKDEAGRVAAVLKREMESAVELDVPLEVEVGTGGNWMDVKK"
     gene            complement(9453..9833)
                     /locus_tag="AC159P_10830"
     CDS             complement(9453..9833)
                     /locus_tag="AC159P_10830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695985.1"
                     /translation="MRESFATLLSRCLRLRCPVCGQSSIVRAPFHIRHQCPSCRALFK
                     REDGFFVGAIAVNVVTTEAVIMLVYVASMPFIGTRFDLVLTALLVIALLFPVAYYHHS
                     WSVWLAFDHLIEKLPKAGEGGGVR"
     gene            complement(9833..10495)
                     /locus_tag="AC159P_10835"
     CDS             complement(9833..10495)
                     /locus_tag="AC159P_10835"
                     /inference="COORDINATES: protein motif:HMM:NF017496.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HIR3695986.1"
                     /translation="MSTLTTSYLDIVERLPAGAMLRLQNVGWDEYEHLLSQMDTLPGR
                     RVSYDRGRLVIMSPSLEHEEYKETIYSLVRALSEELGVTLESRGQATFKRKLLKQGAE
                     PDTCFYVQNASRLIGRRTIELGEDPPPDVVVEIDTTNESLDKFHIYAALGVPEIWRYD
                     GERAFFYQLVGEEYQVMDNSLAFASLAATDLARFVELSKTEGQTAALAAFRGEVRARL
                     RP"
     gene            10819..>11613
                     /gene="lpxC"
                     /locus_tag="AC159P_10840"
     CDS             10819..>11613
                     /gene="lpxC"
                     /locus_tag="AC159P_10840"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: protein motif:HMM:TIGR00325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103117 - UDP-3-O-acyl-N-acetylglucosamine
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HIR3695987.1"
                     /translation="MIRQTTLASPVETTGLGLHTAVPVRLRLAPAPPDTGYVFLRTDL
                     DGFEIPASVECISHCSYATTLMRTGVMLNTVEHLLSALRGCGVDNAYVEVDNLELPIM
                     DGSAEVFVGLIGRAGVAEQPAARRALRVRARVEVVEGARRISVEPHEGYEIDCAIDFR
                     HPLIGVQHYRFDALDAEAFAREIAPARTFGFTEEIDALRRANLIRGGSLDNAIVLTPT
                     GMLNETPLRWRDEFVRHKILDIVGDLALLGMSLLGLVRAERSGHLLH"
BASE COUNT         1794 a         3958 c         3878 g         1983 t
ORIGIN      
        1 gcgcccaggg gcggggcggg ggcgattccg ggcgtgcgcc gctaccgctc ctcggacggc
       61 ctcgacatcc tcgtcggccg cggctcgcgc gacaacgacc acctcacctt ccgcgtcgcg
      121 cgctcgcagg acacatggct ccacgccgcg gactactccg gctcgcacgt cgtcgtccgc
      181 aaccaccggc gcgacgcaga cgtgccgcac cgcaccgtca tcgaggccgc gcagctcgcc
      241 gcgcacttca gtcaggcgcg caaagactcg aaggtcgccg tcaactacac gcagcgcaag
      301 ttcgtctcca agcccaaagc cgccgcgccc ggcctcgtcc gcctctcctc cttccgcacg
      361 ctcctcgtcg agccgcgcga agagttggag cggctgtgag gaagaacagc aagcagcagg
      421 cagcaggcag tcaaggcaaa cggagcgaag cagcttgaga gctaaagtgt cgagcatctt
      481 cgctcatcga gcagtcggag aaagacgcac ggcactttac ctctcaagct gcttcaccct
      541 tctgccttct gccttctgac ttctgactcc tgacttctgc ttttactgcc cgcggcctgc
      601 tgccgactgc ctgctgtgtt attttatttc caacattccg gggagggttt tggatgagca
      661 ccaatattat cgagtcgttc gggaagaggg cggcgagcgt gttggacgac atcgcggacg
      721 cggtcgtcgg caaggcttcg actctgcgcg acgggacgga gcacacgggc gtggagctgc
      781 cgcgcgacct ctacgcgcac gacggcacgc agaccgagtg gtggtactac acgggccacc
      841 tgcaaacgca aacgggtcgc cgcttcggct tcgagctggt cttcttcaag cggcgcacgg
      901 acctcgaccg cttcggcgtc gtgccgctgc ggctcatcgc gaacccgctc tacctcgcgc
      961 acttcgccgt cacggacgag tcgcgcggga gctttcgcta cgagcaccgc aagagcgcca
     1021 acggcccact cgaccccgcg gcccgcacga gcgcgaagca ctactacctg cggctcggca
     1081 actggacggt gcgcgaggcc cacggcctgc acctcttgcg cgcgacgctc ggcgacgacc
     1141 tcgtcttcga ggcggcgctg aagccgacga aggcgccggt cctgaacggc gacgcgggca
     1201 tcggcgtcag ctacaaggat cgcggcgagg cttcgcgcta cttctccttc acgcgcttgg
     1261 aggccgaggg cgacatcacc tggcacgggc gcaccgaaca cttcaagggt gcggcgtgga
     1321 tggacaggga gttcgggacg tggaagacga ccgaagacca gaagggatgg gactggttca
     1381 gcgtccagct cgacgacggg gcggaggtga tggtctacca catccgcgac cgcgccgggg
     1441 cgccgagcgc tttttcgagc ggcacctacg tcgatgccgc cgggcgctgg acgcacctca
     1501 cgcgcgccga cttcgacctc gacacgaccg agcgctggcg cagcccgcac accggcgccg
     1561 actacccgag cggctggcgc ctgcgcgtcc cgcgcttcgg catcgacctc gaagtcaagc
     1621 ccgtcatcaa ggatcaggaa ctcgacacgc gcggcacgac gatgatcgtc tactgggagg
     1681 gcgcgtgcac cgcgcgaggc acgcacggcg gccacaagac ggcgggccgc gcctacgtcg
     1741 agttggtcgg ctacgaccgc tcgcacgagc agccctcgct ctcgaccttc atcttcggcg
     1801 gcgccctcga ccgccgctgg cgcagcatct tcggttaacg gattgcgccc ttcgcatcgg
     1861 gcagcgagac ttcgtcgccg cgtagcggcg attgaatgta gcccggcctt tcaaggccgg
     1921 gagaaacatg cgacaagatt acggcgtcgc gtcagcgacg cttgaagctt cgctcaagcg
     1981 tcgctgacgc gacgcgggcc gacagccacg actgacccgg ccttgaaagg ccgggctaca
     2041 ttcaggcgcc gctacgcggg tcgttttgcg cggcccacat ttattcaacc gtcgtcatga
     2101 tgttgcctga agtcatcacg agcgtcgagc aggtcacgcc ggagtggttg acggagacgc
     2161 tccgccgcaa cggctttctc gaacgtggcc gcgtcaagga cgtgtcggtg gaagtgacgc
     2221 gcacgatgac ggtctcgatt gtctcgcggc tcgtggcctc ttacgcgccg ggcgccgcgc
     2281 cgcacgcgcc cgcgcacctc ttcctgaagc tctccaaccc cgacttccag cccgacgctc
     2341 cgtgcgaccg cccgcccgcg gagatcgttt tttacacttc ggtcgtgccc gagatgcccg
     2401 acccgcccgt ggtgcgctgc tacgactccg cgtactcgcg ctcgacgggg cgctcgcacc
     2461 tcctgctcga agacctgtcc gagacgcacc aacagccctc gccgcatctg ccgccgaccg
     2521 tcgccgagag cgagcgggag gttgatgcgc tcgccgcggt tcacgcgcac tggtgggagc
     2581 acccgcggct cggcaaagac atcggcaggc tgctcggcgc aagtgacgcg gaagagatcg
     2641 cccgccgcaa cgcgtccgac ttcgcgcgct tcgcggactt cctcggcgac cgcctctcgc
     2701 ccgcgcggcg cagcctgtac gagcgggcct tctccgtctt ccctaagccg tggcaacgcc
     2761 tgaccaaagc gaagggcttg acactcacgc acggcgatgc gcacttcggc aacttcctcc
     2821 acccgctcga cgccgagggc ggtcgcacgt acctcgtcga ttggcagttg tggcacccgc
     2881 acatcggccc gcgcgatctg gcatacatga tgacgctctt ctggtatccc gagcgcaggg
     2941 cgcggatgga agaaacgctg ctgcggcgtt atcatcgcgg actcgccgcg ggcggcgtcg
     3001 gcgattacac ttgggaagag tgctttgaag actaccgctg gtcggcgctg agaaatatct
     3061 tcatcccgat tttgcagtgg acgaggggac agccgggttg gctctggtgg tcgcaactcg
     3121 aaaaatctct ggcggcgttc gaagacctgc gctgtatcga gttggttgaa gggtgacgga
     3181 gaacatgacg gcgactcgtg acgacaaaca agacacctcg gcgcggctct tcgcgcgcgc
     3241 cgtcgagctg atgccgggcg gggtcaactc gcccgtgcgc gcgttccgtg gcgtcggcgg
     3301 caacccgcgt tttatcaaga gcgcgagcgg cgcgacgatg acggacgtgg acggtcgcac
     3361 ctacgtcgat tacgtcggct cgtgggggcc gatgatcctc ggccacgccg acgcggaggt
     3421 ggtcgaggcc ctgcgcgaag cgctcgtgcg cgggacgagc tacggcgcgc cgaccgagct
     3481 tgagatcgag atggccgagg agatcgcccg cgccgtgccg tcggtcgaga tggtgcggat
     3541 ggtcaacagc gggacggagg cgacgatgtc cgcgctccgg ctcgcgcgcg gcgtgacggg
     3601 gcgcgaccgc atcctcaagt tcgagggctg ctaccacggc cacggcgaca gcctgctcgt
     3661 caaggccggc tcgggcgtgg cgacgctggg cctgcccgac agccccggcg tccccggagg
     3721 cgtcgccgcg aacaccatca ccgcgccctt caacgacgag gaagcgctcg aacgaatctt
     3781 cgcggagcac ggcgagacga ttgccgccgt catcgtcgag ccggtggtcg gcaacatggg
     3841 ctgcgtggcg ccgcgcgcgg gctacctcga caggatgcgc gacctcacgc ggcgccacgg
     3901 cgcgctgctc atcttcgacg aggtgatgac cggcttccgc gtctcgcgcg gcggcgcgca
     3961 ggagctttac aacgtcctgc ccgacatcac gacgctcggc aagatcatcg gcggcggcct
     4021 gcccgtcggc gcctacggcg cgagccgtga catcatgaac caagtcgcgc ccgccgggcc
     4081 ggtctatcaa gccgggacgc tttcgggaaa ccctctggcg atgaccgccg ggctggtcac
     4141 gctccgtcgc ctgcgcgacc ctgcggtcta cgaacggctc gaacgcgccg gccagacctt
     4201 gtgcgaccag ttggccgagg ccgcgcgcga ggcgggcgtc gccacggtga cgaaccgcgt
     4261 cggctcgatg ttcaccacct tcttcacgag cgaacccgtc cacgactgga cgacggctgc
     4321 gaagtccgac cgcggccttt acgcgcagtt cttccacgcc atgctcgacg ccggcgttta
     4381 cctcgcgccc tcgcagttcg aggccgcctt cctcggcgtc gcccacacgg acgaactgat
     4441 cgaacgcacc atcgaggccg cgcgcaaagc cttccgcgcg ctcgccgcgg cgcgcggcga
     4501 ctgaagcgcg ccgccgggaa gcatcccgcc cctcgtcgtc gcgggccacg aaggcggtcg
     4561 ctccggcgcc cctcgcaggc gttcccgtgt ttcgtgctaa gtgccgttgg ccgagagcgg
     4621 caggtctacg gtgaagcggg tgccttcgcc gggctggctg tggacgtcga tgcgaccgcc
     4681 gtgctgttcg acgatctgct tggcgatggc gaggccgagg cccgtgccct tgcgcttggt
     4741 ggtgaagaac ggctcgaaga tgcgcgcgcg ggtgcgctcg tccatgccgg cgccgcggtc
     4801 ggcgatggtg acgcgggcga agggcgcggg gcgcccttcg ccgccgtgcg gcgcgcgcgg
     4861 ggcgttggcc gtgagagtct cggtcgtgag gcggacgggc gagccttcgc cgcccgcgtc
     4921 cacggcgttg gtgacaaggt tgacgaagac ctgccggagc tggtcgtcgt cccagttgcc
     4981 gcgcagcggc tccggcgtgt actgcttctc gacgggcgtg tgcttctcgt tgaggcggtc
     5041 gaagatgagt tcgaggctgc cgtccaggag cgcgtgcagg tctacttcgg agcgcgtcag
     5101 gcgcttggtg cgcgagaact gcgtcacgtc gagcgtgagc ttcgagaggt gttcgatgcc
     5161 gcgctcgacg aggtcgatgg tgttgatcga ctcgcggtcg cgcgccatgt cgcggagcat
     5221 ggcgacgccg aggcggatgc tccccagcgg gttcttgatc tcgtgcgcga tctgcgccgc
     5281 cgactggccg accgccgcga gcgcctcctg ccggcgcaag acctgctcgg cctccgaagc
     5341 ctcggtcacg tccaccatcg tcaacaactg ccccggctcg ccttcgattt cgagcggcga
     5401 cgagtagacg tcgaaggcgc gctcgtcgcc gcgcttgtcg cgccccatat tccagcgccc
     5461 acggtaggtc gcgcgctcga cgcgcgtgga ggtggccgcc gccagcagct taatcccctc
     5521 ggcggtcgtg aagtcgtcgt ggatcgaggt gccgggccgc gcgccgggga agacctcgta
     5581 gaagggggca ttggcgctgc ggatgcgatc ctcgcggtcg atggcggcga tggcgctgac
     5641 catgccttcg agcatctgcg cgctgaactg ccgctcctgc cggagcgcgt cgtagccgcg
     5701 gcgcaactgc cggaagcggc gcagcacttc gaaggtggtc gcggcggcga cgacaaggcc
     5761 cgtgatggcc gcgaggaatt gcaggaaccg aacctcgccc tgcacgcctt tgagttcgtc
     5821 gtcgcgccgc cccctgatgg cctcgctctc gccgcccacc tcctcggaga gcgccttcag
     5881 ctccgcctcc aggtcgcggt aggccgggta gccgttgatc gagtagctgc cggggtcttt
     5941 cgtgatctcg atgtagtcca cgatgcgctg ccgcacgcgg gccttcttcg cctcgtcctt
     6001 cagcgggagg ctcgcgaaga gcggcagcat cttctcgacc gcgccccgct cgttgcgcag
     6061 gcgcaggtcg gagggcggca gcatgacgcc cttcgtgccc gcctcgacct gcccgcggat
     6121 gcgcgcctcc gtgtcgagct tgctcagcgc gagccgcaaa tcgagcaggc gccgcagcat
     6181 ctccgacagc ttccgctcct cctgctcggt cttgaacctg acctcctcca actcgcgccg
     6241 gctcagcacg ccgaggccga tcaccagcgc gatcagcagc gcgaagccga ccagcagcgg
     6301 cagcagcgcc tgccacaaag tcccctgccc cgccgcctcg gacgccgccc gccgctccct
     6361 ctcttcccgc gcacgcgcct cccccgcgtc gccgtagacg acgtgctcgg gcttctgctg
     6421 ttcaccttcc gacataactt aagagccgtg aaccgcgaac cgtgaaccgt gacaagtgaa
     6481 gaaccggttg cttctcgtca ctcgtcacgt gttactcgtc agcgctttgc tccttgtcac
     6541 ggttcacggt ttacggttta cgattcacgg tttacctcac ttcttcactc acttcttcac
     6601 gtccatccag ttgccgcccg tcccgacctc gacttcgagc ggcacgtcga gttcgaccgc
     6661 cgactccatc tcccgcttga ggacggcggc gacgcgcccg gcctcgtcct tcggcgcctc
     6721 gatcagcagc tcgtcgtgca cctgcatcac catccgcgcc ttcaggtttt cgttccgcag
     6781 cgcctcgtca accttgagca tggcgatctt cacgatgtcg ctcgcggttc cttgtatcgg
     6841 catgttaatc gcttcgcgct cggcgcggaa gcggaggttg gcgttgcggt cgttgatggc
     6901 ggggatgggg cggatgcgac cgtagagcga gcggacgtag cccagctcgc gggcccgcac
     6961 aggcacctcg tccatgaagc ggcgcacgcc ggtgtaggtg ttgtagtagt cctcgatgac
     7021 ctgcttggcc tccttgcgcg agatgccgac gcgctgcgag aggccgaacg gctcgacggc
     7081 gtaggcgatg gcgaagttga cgatcttggc gaagcggcgc ttctccttca gctcctcttt
     7141 ggtcttcgcc ttgaagacga ggcgggccgt gcgctcgtgg atgtcctcgc ccttctggaa
     7201 ggcttcgagc atgtgcgcgt cctgcgtgat gtgcgccagc aggcgcagct ccagttgcga
     7261 gtagtccgcg gcgacgaaga cgttcccctc gtcggggacg aacgcgcggc ggatgcgccg
     7321 gcccagctcg gtgcggatcg ggatgttttg taaattcggg ttggagcttg agagccggcc
     7381 cgtcgcggtc accgtctggt tgagttcgct gtggatgcgt ccgtccgcgc cgaccagcgc
     7441 gggcagcgcg tccgtgtagg tcgccttcag cttgtccagc tcgcggtact cgatgatgag
     7501 ccgcggcagc tcgtactgtt gcgccagctc ttcgaggacg gcgcggttgg tcgagacctg
     7561 cccggtcgcc gtcttgcggt tcgtgctgat gttcagctct tgcagcacct cgccgacctg
     7621 cttcggcgag ttgatcttga actcgcggcc cgcctccttg taaatccgct cggtgagctt
     7681 ggccagctcg gcgccgaaga gcgcggacag ctcgccgagc acggaggtat cgacgcgcaa
     7741 ccccgcgcgc tcgatgcggt ggatgagcgg ggcgagcggg atttcgatct cgctgtagat
     7801 ggattcgagg ccctgttcga ggatgcgacc gtggagcacg tcggccacct gcgcggtgaa
     7861 gtccgcggcc tcggcggtgc gccagctcgc ttcgccccac tcttcgccgc gcggctccgt
     7921 ccagccgtcg gccccgacgg cctcgcgcgc caggtcgccg aggtcgtagc ggctgcgcgt
     7981 cgggtctaag aggtaagcgg cgagcagcgt ctcgtccgtc acgccttcga cctcgacgcc
     8041 gagcgtttcc aagagcgcgg tcgcgcgctt caggtcgtgg acggacttcg agaggaggcc
     8101 gttcgagagc acgtcgcgca gcatcgcggc ggcccgcccg gccccgccct cgaactccgc
     8161 gaagttgacg aagtgcgccg tgtgcggcgc gtaggagatg gcgaggccgg cgggcggcgc
     8221 cgactgctcg cgcaccgaag attgttgacc gtcgcccgcg ggcgtcccgt cggcgacggc
     8281 gaacgcccaa tgctccgcgt cccacagctt gcgcacgagc gtttccagct ccgcgaccgt
     8341 gcgcacctgc cggtagttct tcgccacgcc gcccgtgtca acttccgtcg ccgcgtcggc
     8401 gaactcgcgc gtcaaggttt ggaattcgag ctcgcggaag agcttgtagg cggcggcacg
     8461 gtcgggcgga cgcgagcgga gcttctccag atcgagcttg acgttcacgt ccgtcctgat
     8521 ggtcgccaaa tctttcgatt gcagaatcag ctcagcgttg tcgcgcaatg attcgcgata
     8581 ggtcttgtgc ttgacctcct cccaattctt cagtgcgttc tcgatggagc cgaactgctt
     8641 gacgatctgc accgcgccct tcgcgccgat gccgggcgcg ccggggatgt tgtcaaccga
     8701 gtcgcccatc aagccgagca ggtcaaccat ctgcgccggg ggcacgccga agcgcgcctc
     8761 gacccaagcg gcgtcgcacc actcgacggg cggcaccggc tccttgcgct tcacgttctg
     8821 cgagttctgc cgcatgcaga ccacccacgg gtcgcggacg agctgcgtca tgtccttgtc
     8881 gttcgagacg acgacggcct gaaggccggc ttcggcggcc tgcacggcga gcgtgccgat
     8941 cacgtcgtcg gcctcgaagc cgggcgcgtt gaggatgggg atgttgtacg cctcgcagac
     9001 gcggacgatg tagggaatct gcgactccag ctcatcgggc ggcgcggcgc ggttggcctt
     9061 gtagccgacg ttcatctcgt ggcggaaggt cggctcgccc gactcgaaga ccgccgcgat
     9121 gtagtgcggc tgctcgtccg agagcagctt gcggagcatg ttcgtgaaga cgaagacggc
     9181 ctgcgtcacc tgcccctggc tgttcgtcaa aggctcggtg cgcgcgccca tcggcgcgaa
     9241 gaaggcgcgg aagatgtgcg agaacgagtc gatcaggaag acccgctcgg tcggcccctg
     9301 cggcgtagct tttttcggcg gcatgttgct tcgatgataa gtaatgcgtc ggcgggtgac
     9361 aagctagcgg gcggcggtcg gtggaggaaa tacgtgcgac ggacttaatc gacgagtccg
     9421 gcgcgcgcgc ctggtgtcgg ccccccgcga actcaacgca caccgccgcc ctcgcccgcc
     9481 ttcggcagct tctcgatcag gtggtcgaag gcgagccaca cgctccaact gtggtggtag
     9541 taggcgacag ggaacaggag cgcgatgacc agcagggcgg tgagcacgag gtcgaagcgc
     9601 gtgccgatga agggcatcga ggcgacgtag acgagcatga tcacggcttc ggtcgtcacc
     9661 acgttgaccg cgatggcgcc gacgaagaag ccgtcctcgc gcttgaagag cgcgcggcac
     9721 gaagggcact ggtggcggat gtggaacggc gcgcggacga tggacgactg gccgcacacg
     9781 gggcagcgca ggcgcaggca gcgcgacagc agggtggcga agctctctct catcagggcc
     9841 gcaggcgcgc gcgcacctct ccgcggaaag cggcgagcgc cgccgtctgc ccctcggtct
     9901 tgctcaactc gacgaaacgc gcgaggtctg tcgccgcgag cgacgcgaag gcgaggctgt
     9961 tatccatcac ctgatactct tcgccgacga gttgatagaa gaacgcgcgc tcgccgtcgt
    10021 agcgccagat ttcgggtacg ccgagcgccg cgtagatgtg aaacttgtcc agcgactcgt
    10081 tcgtcgtgtc gatctcgacg acgacatcgg gcggcgggtc ttcgcctagc tctatcgttc
    10141 gcctcccgat aaggcgcgaa gcattctgga cgtagaagca tgtatcaggc tccgcaccct
    10201 gcttcaacag cttgcgcttg aaggttgctt gccctctcga ttcgagggtc acgcccaact
    10261 cctctgagag cgcgcgaacc agggagtaaa tcgtttcctt gtactcttcg tgttccagcg
    10321 atggactcat gatgactagc ctcccgcggt cgtagctcac gcgacggcca ggaagcgtgt
    10381 ccatctgaga gagcaggtgc tcgtactcgt cccacccgac gttctgcagc cggagcatgg
    10441 ctcccgcggg caggcgctcg acaatgtcca gatagcttgt ggtcaacgtg ctcatggtgc
    10501 tgtgactccg aatcgacgac caaccgcaac ggggcgcggc ctcattatga accgcgtgaa
    10561 ccgaccgggc aagattcgat tcgcgacgga tgcgccgcgc gttggctgcg ccttcggctg
    10621 ttgcgcgcgg gccacgcgtg gcggcgttcg atgttgcggg ggcgccacaa tcgccgcgcg
    10681 gtgaaccggc acttcgcgcg caaaacacgg ctatcctccc ggttcgacgc gcggcacggg
    10741 cgttgccatc attattcgtg ttcgaccacc tacgggcgtg tccccgcacg ccggacgcga
    10801 aaggcatgac gcctcgtttt gattagacag actactcttg cctcgcccgt ggagaccacc
    10861 ggcctcgggc tgcacacggc ggtgcccgtc cgcctgcggc tcgcgcccgc gccgcccgac
    10921 acgggctacg tgttcctgcg cacggacctc gacggcttcg agattccggc ctcagtcgag
    10981 tgcatctcgc attgcagcta cgccacgacg ctgatgcgca cgggcgtcat gctgaacacg
    11041 gtcgagcacc tgctgtcggc gctgcgcggg tgcggcgtgg acaacgccta cgtcgaagtc
    11101 gataaccttg agctgccgat catggacggc tcggcggagg tgttcgtcgg gctgatcggg
    11161 cgcgccgggg tagccgagca gccggcggcg cggcgcgccc tgcgcgtgcg cgcgcgggtc
    11221 gaggtcgtcg agggcgcgcg ccgcatctcg gtcgagccgc acgagggcta cgagatcgac
    11281 tgcgccatcg acttccgcca cccgctcatc ggcgtgcagc actaccgctt cgacgcgctc
    11341 gacgccgaag ccttcgcgcg cgagatcgcg cccgcgcgca ccttcggctt cacggaggag
    11401 atcgacgcgc tcaggcgcgc gaacctgatc cgcggcggct cgctcgacaa cgccatcgtg
    11461 ctcacgccca ccgggatgct caacgagacg ccgctgcgct ggcgcgacga gttcgtgcgc
    11521 cacaaaatcc tcgacatcgt cggcgacctc gcgctgctgg ggatgtcgct tctcggtctc
    11581 gtcagggccg agcgcagcgg ccacctgctg cac
//
LOCUS       DBNGOH010000558         3103 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3932510, whole genome shotgun sequence.
ACCESSION   DBNGOH010000558 DBNGOH010000000
VERSION     DBNGOH010000558.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3103)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3103)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3103
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3932510"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3026
                     /locus_tag="AC159P_10845"
     CDS             <1..3026
                     /locus_tag="AC159P_10845"
                     /inference="COORDINATES: protein motif:HMM:NF051698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR3695988.1"
                     /translation="ARNTGTGQTRTTQTNEDGNFNISELTPGTYEVTVEAPNFSKALV
                     KDLELNIGAARTLNFDLKPGQLTEVVEVAGGLNEIQTTSSQIGGVVTPLEVENLPLLN
                     RTFAALSVVMPEARPVGNFDPTKTRVGNVAFNGGDGRQVDVNVDGGNNKDNVVGSLLQ
                     NFAYESIQEFQVLQHRWTAESGRAVGGVVNVITKSGGNEFHGSFFGNFRDEALRKLDF
                     FEEQRQAATPSFEKPSFNRQEIGGSFGGPIKRDRLFFFGALERFRERQFNALSAAAAA
                     QLPAIPGIGTVGDIPSPYDDWLLTAKVDHRLDDDQTMFYRYSYQKNSSPNDQIAVPAS
                     ADITGGNTNDNKLHSFVANHTWTISPTKLNQWSFHFQDFSNFILGVTETPNIVFPSVQ
                     TGRNANVPQATLIKKFQMRDDFSANAGQHSLKAGINYIHTKLDGFFFFGAQGYQIQFF
                     ADPLVIRNNTNGLYPQGFATPGAVRQITFSDGRGDHKQTIHQLAFYIQDDWKVTQRLT
                     FNLGLRWDANIRNLPRQDDNRTILLLQQLNHPLARAITGDPDKLTRTTPSWTEFQPRV
                     GFALDPTGAGRTVIRGGYGIFYDQLFQNLTLFSLTQSNPELFQTALTLINTAVGVGQL
                     STFRFGVDPLPPTPAGFSFSDLAFGGFGRINDPDAKEPYVQKFSIGFETRLWENTTLS
                     SDFVHTLGLREPRVQVINPRIRQLCDPGFPGSNPASPQCVRGANTRLLDRAFVDAGFP
                     IIGNTARVEQINMIGTTNRSLFDSWATTLRHRTRRMTLSASYVLADSRSWGGQPVASY
                     SGNGIATTPDNQFKPEEFGPSRIDERHRIVASGVFNLPHGFQLAPIVQLASARPYSLN
                     LGLDIDGDGLATNDRICEGTDPAAVFAARGNSATLAALNPLGCRQVQVNSVREGFITD
                     AAGQIIDTRSGRYFNVDLRAQKAFRIGERFQIKAYADFYNLFNTENLSLSNRLGNTAA
                     TSAGFYQQPNSLYGPGFGPPVGRPLTLQLGARLDF"
BASE COUNT          554 a         1205 c          872 g          472 t
ORIGIN      
        1 ccgcgcgcaa caccggcacc ggccagacgc gcaccaccca gaccaacgag gacggcaact
       61 tcaatatctc ggagctgacg cccggcacct acgaggtcac ggtcgaggcg cccaatttca
      121 gcaaggcgct cgtcaaagat ttggagctga acatcggcgc ggcccgcacg ctcaacttcg
      181 acctcaagcc cggccagctc acggaggtcg tcgaggtcgc gggcggcctc aacgagatac
      241 agaccaccag ctcgcagatc ggcggcgtcg tcacgccgct cgaagtcgag aacctcccgc
      301 tgctgaaccg caccttcgcc gcgctctccg tcgtcatgcc cgaggcgcgg cccgtcggta
      361 acttcgaccc gacgaagacg cgcgtcggca acgtcgcctt caacggcggc gacgggcggc
      421 aggtggacgt gaacgtggac ggcggcaaca acaaggacaa cgtcgtcggc agcctcttgc
      481 agaacttcgc ctacgagtcg attcaggagt ttcaggtctt gcagcaccgc tggacggcgg
      541 agtcggggcg cgccgtcggc ggcgtcgtca acgtcatcac caagtcgggc gggaacgagt
      601 tccacggctc gttcttcggc aacttccgcg acgaggcgct gcgcaagctc gacttcttcg
      661 aggagcagcg gcaggccgcc acccccagct tcgagaagcc ctcgttcaac cggcaggaga
      721 tcggcggctc cttcggcggc cccatcaagc gcgaccggct cttcttcttc ggcgcgctcg
      781 aacgcttccg cgagcggcag ttcaacgccc tgtcggcggc ggcagccgcg cagctcccgg
      841 ccatccccgg catcggcacc gtcggcgaca tcccctcgcc ctatgacgac tggctgctca
      901 ccgccaaggt cgatcaccgc ctcgacgacg accagacgat gttctatcgc tactcgtacc
      961 agaagaacag ctcgcccaac gaccagatcg ccgtccccgc cagcgccgac atcacgggcg
     1021 gcaacacgaa cgacaacaag ctgcacagct tcgtcgccaa ccacacgtgg acgatcagcc
     1081 cgaccaagct caaccagtgg tcgttccact tccaggactt cagcaacttc atcctcggcg
     1141 tgaccgagac gccgaacatc gtcttcccct cggtgcagac cggcaggaac gccaacgtgc
     1201 cgcaggcgac gctcatcaag aagttccaga tgcgcgacga cttctcggcc aacgcggggc
     1261 agcacagcct gaaggccggc atcaactaca tccacacgaa gctcgacggc ttcttcttct
     1321 tcggcgcgca gggctaccag attcagttct tcgccgaccc gctcgtcatc cgcaacaaca
     1381 cgaacggcct ctacccgcag ggcttcgcca cgcccggcgc cgtccgccag atcactttct
     1441 cggacgggcg cggcgaccac aagcagacca tccaccagct cgccttctac atccaggatg
     1501 actggaaggt gacgcagcgc ctcaccttca acctcggcct gcgctgggac gccaacatcc
     1561 gcaacctgcc gcggcaggac gacaaccgca ccatcctcct gctccagcag ctcaaccatc
     1621 cgctcgcgcg agccatcacg ggcgaccccg acaagctcac gcgcacgacc ccgagctgga
     1681 ccgagttcca gccgcgcgtc ggcttcgccc tcgacccgac gggcgccggg cggacggtca
     1741 tccgcggcgg ctacggcatt ttctacgacc agctcttcca gaacctcacg ctcttctcgc
     1801 tgacgcagtc gaaccccgag ctgttccaga cggcgctgac gctcatcaac acggcggtcg
     1861 gcgtcggcca gctctcgacc ttccgcttcg gcgttgaccc gctgccgccg acccccgcgg
     1921 gcttctcctt ctccgacctc gccttcggcg gcttcgggcg catcaacgac ccggacgcca
     1981 aagagcctta cgtgcagaag ttctccatcg gcttcgagac gcggctctgg gagaacacga
     2041 cgctctccag cgacttcgtc cacacgctcg gcctgcgcga gccgcgcgtc caggtcatca
     2101 acccgcgcat ccgtcagctc tgcgaccccg gcttccccgg cagcaacccc gcctcgccgc
     2161 aatgcgtgcg cggcgccaac acgcgcctgc tcgaccgcgc cttcgttgac gcgggcttcc
     2221 cgatcatcgg caacacggcg cgcgtcgagc agatcaacat gatcggcacc accaatcgct
     2281 cgctcttcga cagttgggcg acgaccctgc gccaccgcac gcgccggatg acgctctcgg
     2341 ccagctacgt gctcgccgac tcgcgctcgt ggggcgggca gcccgtcgcc tcctacagcg
     2401 gcaacggcat cgccaccacg cccgacaacc agttcaagcc cgaggagttc ggcccgtccc
     2461 gtatcgacga gcgccaccgc atcgtcgcca gcggcgtctt caacctcccg cacggcttcc
     2521 agctcgcgcc catcgtgcaa ctggcgagcg cgcgccccta ttcgctcaac ctcggcctcg
     2581 acatcgacgg cgacggcctc gccaccaacg accgcatctg cgagggcaca gatcccgcgg
     2641 cggtcttcgc cgcccgcggc aactccgcca cgcttgccgc cctgaacccg ctcggctgcc
     2701 ggcaggtgca ggtcaacagc gtgcgcgagg gtttcatcac cgacgccgcc ggccagatca
     2761 tcgacacgcg cagcggtcgc tacttcaacg tcgatctgcg tgcgcagaag gcgttccgca
     2821 tcggcgagcg cttccagatc aaggcgtacg ccgacttcta caacctcttc aacaccgaga
     2881 acctctcgtt gtccaatcgc ctcggcaaca ccgccgccac gtccgccggg ttctaccagc
     2941 agccgaactc gctctacggc cccggcttcg gcccgcccgt cgggcgcccg cttacactgc
     3001 aactcggcgc gcgcctcgac ttctaatcga ctgcgcgcga cgtgttcgat tcttcgggcg
     3061 ccggggcttg aaacccccgg cgcctttttt gggcgggctt gca
//
LOCUS       DBNGOH010000559         3716 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2281654, whole genome shotgun sequence.
ACCESSION   DBNGOH010000559 DBNGOH010000000
VERSION     DBNGOH010000559.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3716)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3716)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3716
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2281654"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..515
                     /locus_tag="AC159P_10850"
     CDS             <1..515
                     /locus_tag="AC159P_10850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695989.1"
                     /translation="PEELMKTQTEKILAEQRQNMYNAQREAEDKRIQLEKTKAHADNQ
                     RDLMAATVGVEISTRRSEQRKAEAEGEAHYILATGRAEAEKVRLMGEAQGVAYKEQVN
                     ALGPQGVALVETLKVIGEKQVRITPDILASGGAGDGAGGGIGQLLLLSLFRDRLGVET
                     PTGNGAKTLS"
     gene            828..1391
                     /locus_tag="AC159P_10855"
     CDS             828..1391
                     /locus_tag="AC159P_10855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695990.1"
                     /translation="MAELFAGISALTVFLGIAAVGLIFLVVSFVFGELLDNFGLHVGG
                     DSGLDGHGFIDSRALAVLITAFGGFGAIGIQLGLGVPASSLLGLGSGIVLGGLVTLFG
                     RFLYSQQASSSVGNAQLVGRTAQVTVMIPSGGIGQVSCRVGEERVEKLARSRESVELK
                     PGMLVRIEEIAGDSAIVSPAADAQHYK"
     gene            1444..3087
                     /locus_tag="AC159P_10860"
     CDS             1444..3087
                     /locus_tag="AC159P_10860"
                     /inference="COORDINATES: protein motif:HMM:NF013324.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0016600 - flotillin complex [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HIR3695991.1"
                     /translation="MQLDSQTLLIPIIVLVVSLAIIIAFWLLSRNYIKVPPNEAAVIS
                     GRSRKLSDGTTVGYRLVRGGATLIFPFLEKVDYLNLNVLTVPLATSRAYTGQGVPVSV
                     KAVANVKIKGDDESLRAAAERFLGMKQEEFHRLVFQTLEGHLRAILGTLTVEEINNDR
                     QSFAQKLTTEAAGDLEKMGIGLDALTIQEISDEEGYLDALGKRRTAEVQRDAEIGEAE
                     AKRDSKIKASLAMQEGEKVKLESEALIAASTRETEVKRAQYMAEIQREKAKSEQSGPL
                     ADAKARQEVVAEEVRIERIRTQEQIAVQEQEVLRREKELEATVVKKAEADRRAAVLRA
                     QGEQEAAILAAEGRKQAMIAAAEAESQKLQREGQGRASAIEAEGRAEAEKIKALGLAE
                     AEKIQAQGLAEAKSIEARGLAEAAAIQKKADAWREFNDAARLQTVLEKLPGIIEATAP
                     ALRAVAEPLANIDKVVMIDHGGADGNGHGAGGLNRYAQTGPTLIFGLLQQLQALGLNV
                     PEVLAQLGVKLDGEKAASIPATATTKTVELENGNRPDEK"
     gene            complement(3254..>3716)
                     /locus_tag="AC159P_10865"
     CDS             complement(3254..>3716)
                     /locus_tag="AC159P_10865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695992.1"
                     /translation="LIVTNNSTHNPVALLYAGSSTSTIGNPINQVLTQLSTALGGGRT
                     LSFGAGGGNFAEVFDTSSGVRPFLPEVAQMPELPEQAVEHAGQVLERHRDELMSRAAI
                     IGVGVGRSDANESEAAIIVYLDKTARGRGRVPETIDGLRVLVVETDPFVAF"
BASE COUNT          703 a         1230 c         1236 g          547 t
ORIGIN      
        1 tgccggaaga gctgatgaag acgcagaccg agaagattct cgccgagcag cgccagaaca
       61 tgtacaacgc gcagcgcgag gccgaggaca agcgcatcca gctcgaaaag accaaggccc
      121 acgccgacaa ccagcgcgac ctgatggccg ccacggtcgg cgtcgaaatc tcgacgcgcc
      181 gctccgaaca gcgcaaggcc gaggccgagg gcgaggcgca ctacatcctc gccacgggcc
      241 gcgccgaggc cgagaaggtg cggctcatgg gcgaggcgca gggcgtcgcc tacaaggagc
      301 aggtcaacgc gctcggcccg cagggcgtcg cgctcgtcga gacgctcaag gtgatcggcg
      361 aaaagcaggt gcgcatcacg cccgacatcc tcgcctccgg cggcgcgggc gacggcgcgg
      421 gcggcggcat cggccagctt ctcctgctca gcctcttccg cgaccgcctc ggcgtcgaaa
      481 ctccgacggg aaacggcgcg aagacgctca gttagagaac tgcatggtca aaggggcggt
      541 cagtccggtt cgtgactggc cgccccttcc gtattgccgg ggcgtctctc ggcgccctct
      601 gcgttaaagc tcacctcggt gacgcgcacc cgcaatttga tgtgggtcgc cctagaactt
      661 tcaaaaagat ggggcgtata atcctgtaag caccggttct gacgcgcgat gacatggacg
      721 gtgatcttcc tcctcgatgc cgcgtcagac gtgggacgca gcaacatgac gccgccctaa
      781 agcatcacga actacccgca caccgacttg ttccgaggaa aagaaaagtg gcagaactct
      841 tcgccggcat cagcgccctg accgtctttc tgggcatcgc agccgtcggg ctgatttttc
      901 tcgtcgtctc gttcgtcttc ggcgagctgc tcgacaactt cggtcttcac gtcggcggcg
      961 acagcgggct tgacgggcac ggcttcattg actcgcgcgc gcttgccgtc ctgatcaccg
     1021 ccttcggggg tttcggtgcc atcggcatcc agttgggcct cggcgtgccg gcgagttcgc
     1081 tgctcggact gggcagcggg atcgtcctcg gcgggctggt cacgttgttc gggcgcttcc
     1141 tctacagcca gcaggcttcg agttcggtcg gcaacgcaca gctcgtgggc cgcacggcgc
     1201 aggtgaccgt catgatccct tcgggcggca tcgggcaggt cagttgccgc gtcggcgagg
     1261 agcgggtcga gaagctcgcc cgctcgcgcg agagcgtcga gttgaagccc gggatgctgg
     1321 tgcgcatcga agagatcgcc ggtgactcgg ccatcgtcag tcctgccgcc gacgcacaac
     1381 actacaagta gcgcgccgcg ggtgcatccg gcttccgccg cctgcgtgcg aggagacaaa
     1441 ctcatgcaac tggatagtca gactcttctc atcccgatca tcgtcctcgt cgtctctctg
     1501 gcgatcatca tcgccttctg gctcctgagc cgcaactaca tcaaggtgcc gcccaacgag
     1561 gccgccgtca tttcgggccg ctcgcggaag ctgtccgacg gcacgaccgt cggctaccgc
     1621 ctcgtgcgcg gcggcgcgac cctcatcttc ccctttctgg agaaggtcga ttacctgaac
     1681 ctgaacgtcc tcaccgtccc gctcgcgacc tcgcgcgcct atacggggca gggcgtgccc
     1741 gtctccgtga aggcggtggc gaacgtcaag atcaagggcg acgacgagtc tctgcgcgcc
     1801 gccgccgagc gcttcctcgg catgaagcag gaggagttcc accggctcgt cttccagacc
     1861 ctcgaaggcc acctgcgcgc catcctcggc acgctgaccg tcgaggagat caacaacgac
     1921 cgccagagct tcgcgcaaaa gctcacgacc gaagcggcgg gcgacctcga aaagatgggc
     1981 atcggactcg acgcgctgac cattcaggag atcagcgacg aggaaggtta tctcgacgcg
     2041 ctcggcaagc ggcgcacggc ggaagtccag cgcgacgccg agatcggcga ggccgaggcc
     2101 aagcgcgact ccaagatcaa agcctcgctc gccatgcagg agggcgaaaa ggtcaagctc
     2161 gaatccgaag ccctgattgc cgcctcgacg cgcgagaccg aggtcaagcg ggcgcagtac
     2221 atggcggaaa tccagcgcga gaaggcgaag tccgagcagt cggggccgct cgccgacgcg
     2281 aaggcgcggc aggaagttgt cgccgaagaa gtccgcatcg agcgtatccg cacgcaggag
     2341 cagatcgccg tgcaggagca ggaagttctg cgcagggaga aggagttgga ggcgaccgtc
     2401 gtcaaaaagg ccgaggccga ccgccgcgcc gccgtcctgc gtgcgcaggg cgagcaggag
     2461 gccgccatcc tcgccgccga ggggcgcaag caggcgatga tcgccgccgc cgaggccgag
     2521 agccagaagc tccagcgcga gggtcagggc cgcgcctcgg ccatcgaggc cgaaggcaga
     2581 gcggaggccg agaagatcaa agccctcggc ctggccgagg ccgaaaaaat ccaggcgcag
     2641 ggtctcgccg aggcgaagtc catcgaggcg cggggcctcg cagaggccgc cgcgattcag
     2701 aagaaggccg acgcgtggcg cgagttcaac gacgcggcgc gtctgcaaac ggtgctcgaa
     2761 aaactgcccg gcatcatcga agccacggcc ccggcgttgc gcgccgtcgc cgaaccgctt
     2821 gccaacatcg acaaggtcgt catgatcgac cacggcggcg cggacgggaa cggccacggc
     2881 gcgggcggcc tcaaccgcta cgcccagacc gggccgacgc tgatcttcgg ccttcttcag
     2941 cagttgcagg cgctcggcct gaacgtgccc gaagtactgg cgcagctcgg cgtaaagctc
     3001 gacggcgaaa aagccgcgtc gatccccgcg accgctacca cgaagaccgt cgaattggaa
     3061 aacggcaacc gtccggacga gaaataaaac gggcaccgtg acggcctcgg aggccgggta
     3121 cgtgtcacga ccggcgttga gtggaaggca agatcgttct cgacgcagca gcgcgccgga
     3181 agcggcgggc gacagaaaaa ggcgggcctg acagtagaag tcaggcccgc ctttgtcatg
     3241 cccgcgccaa cgctcagaag gcgacgaacg ggtcggtctc gacgaccagc acgcgcaggc
     3301 cgtcgatggt ctccggcaca cgccccctcc cgcgcgccgt cttgtccagg taaacgatga
     3361 tcgccgcctc tgactcgttc gcgtccgagc ggcccacgcc gacgccgatg atggccgcgc
     3421 gtgacatcaa ctcgtcgcgg tgccgttcga gcacctggcc ggcgtgctcg accgcctgtt
     3481 cgggtagttc gggcatctgc gccacctccg gcaagaaggg ccgcactccc gaggacgtgt
     3541 cgaagacctc ggcgaaattg ccaccgcccg cgccgaagct gagcgtcctg ccgccgccga
     3601 gcgcagtgct gagctgcgtc agaacttggt tgatggggtt gccgatggtc gaggtgctgc
     3661 tccccgcgta gagcagcgcg acggggttgt gcgtgctgtt gttggtcacg atgagc
//
LOCUS       DBNGOH010000560         2684 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3015064, whole genome shotgun sequence.
ACCESSION   DBNGOH010000560 DBNGOH010000000
VERSION     DBNGOH010000560.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2684)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2684)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2684
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3015064"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="AC159P_10870"
     CDS             complement(<1..189)
                     /locus_tag="AC159P_10870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3695993.1"
                     /translation="MTNLSKARFAGLACTLGGLLFLFYFTGLNALIAALTPPLEAARV
                     PALKMLLTCVAAICLAGGP"
     gene            complement(319..822)
                     /locus_tag="AC159P_10875"
     CDS             complement(319..822)
                     /locus_tag="AC159P_10875"
                     /EC_number="5.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019013.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HIR3695994.1"
                     /translation="MIDVAAGEGRIAELNKAAVLSMIAAAGREGLTAQADFFAERVTN
                     HGIPGTREDVRMVLLDISTTFPDARFELLDVVAEADWVVVRCFLSGTHLGVGRHPFVH
                     EGLLAGVAPTGRRIRVQHVHMFRLRDALVVEHWGCRDDVEMMRQLGLPLRIAGSREAE
                     KLSHKGE"
     gene            complement(1627..>2684)
                     /locus_tag="AC159P_10880"
     CDS             complement(1627..>2684)
                     /locus_tag="AC159P_10880"
                     /inference="COORDINATES: protein motif:HMM:NF012770.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR3695995.1"
                     /translation="AVGSERAALCGVSEGGPMSALFAATYPEKTSALVMIGTYAKRTQ
                     TADYPWAPTAEERASFLEELRRDWGGAVGLEERAPSAAGDPSFRAWWAEYLRMGASPG
                     AAVALTKMNAEIDVRHVLPSIRVPALVIHRAGDACLPVGGGRYVASHIPGAKYVELPG
                     RDHLPFVGDQDAVLDEVEVFLTGARHVAEVDRVLTTVMFVLIDEGDTAASPSRRREMR
                     QLYQSYARRELAHFRGREMEIAGDRLFATFDGPARAIGCARAVVSTAARMGLLARAGL
                     HTGECDVIGEKVGGTGVTLAEGVAALASPGETLISHTVKDLVAGSGVEFKPRGARVFG
                     NLPGEWLLFAVEHVADS"
BASE COUNT          493 a          886 c          849 g          456 t
ORIGIN      
        1 cggccccccc gccagacaga tggcggcgac gcacgtcaga agcatcttca gcgccgggac
       61 ccgggccgct tctaacggcg gggtcagcgc cgcgatgagg gcgttcagac ccgtgaagta
      121 gaacagaaaa agcagcccac ccagcgtgca cgcgagcccg gcaaagcggg ccttcgataa
      181 gttcgtcacg gatgaaaaat acctttccta caacttaaat atttccggcg gggttcttat
      241 ggattacaac cggccgccga atatctccgg atgaattggc cgccccgccg aagccccgac
      301 aaggtatcgg aaactggctt actcaccctt gtgggatagt ttctcagcct ctcgtgaccc
      361 ggcaatgcga agcggtaacc cgagttgccg catcatctcg acatcatcgc ggcagcccca
      421 gtgttcgacg accagcgcgt ctctgagacg aaacatgtga acgtgttgca cgcgaatgcg
      481 cctgccggtc ggcgccacac cggcgagcag accttcatgg acgaaggggt gccggcccac
      541 gccgaggtga gtgccgctca ggaaacagcg caccaccacc caatcagcct cggccaccac
      601 gtcgagcaat tcgaacctcg cgtcaggaaa ggtggtcgag atatctagca gcaccatccg
      661 gacgtcttcg cgggtgccgg ggatgccgtg gttcgtcacg cgctcggcga agaaatcggc
      721 ctgcgccgtc aacccctccc gcccggccgc cgcgatcatg ctcaggaccg cggccttgtt
      781 caactcggcg attcggcctt cgcccgccgc tacatcgatc atcagctcac ttctccttca
      841 tcagagggcc ctcctgtggg agagccgtgt cgaggcagtc atccccgtcg cgggagaaag
      901 taagccagga gcgggccgaa aatctttaga gagttatgaa atttttctga atgcgcaaac
      961 agcttgattt gaagccgttc agggcgtatt gggaaggcgt gttggggttg gaagagtagc
     1021 ggattgatgc caaacgtaga gtgacctcaa acgatgggtg gaaggtcatg gtccaacgct
     1081 gaatcggctc gaagggagtt ccatagcata tcgaaacaca cgcctgacac ctgctcaacg
     1141 aaagcccctg gctcccggcg ggtgatgcgg gtcgtccacc cgaacggttt tgcagaaatc
     1201 ggcccgtacc cctcacggaa ggtcaagcta ggaggtgtcg gtgagcgaca ttccaaagag
     1261 gtagtcaaag cgcccgagtt ctcccgcact ttccgggtgg tgcacaaagc ggaaggcagg
     1321 cgcccccagc tccgggtctg ctcgtgcccg aatgagtacg tcacctgctg cgcctgaggt
     1381 gaccagccct cctctccgtc ctcgcggggt ggtactggtg tctggaggta gtcggcctcc
     1441 cgccgttggc acggcggatg ccggttcatg accgctactg gggtgagttg gataagctcg
     1501 actgcgggag gtctctctga tggcttggag acatgtccca gtgtgggcgg ccatttaaca
     1561 cttctacccc atcccgccct ttatagcagc gccagctggt aactctttta tgagaaggct
     1621 gtcgccttag ctatctgcca cgtgctcgac agcgaaaaga agccactcac cgggcagatt
     1681 cccgaacacg cgcgcgccgc ggggcttgaa ctcgacgccg gagccggcga cgaggtcctt
     1741 cacggtgtgt gagatcaagg tctcgccggg cgacgcgagc gcggcgacgc cctccgcgag
     1801 ggtgactccg gtgccgccca ccttctcgcc gatcacgtcg cactcgcccg tgtggaggcc
     1861 ggcgcgggcg agcaacccca tgcgcgccgc ggtgctgacg acggcgcggg cgcagccgat
     1921 ggcgcgcgcc ggcccgtcga aggtcgcgaa aaggcggtcg cccgctatct ccatctccct
     1981 tcccctgaag tgcgcaagct cacgccgggc gtaagactga tagagttgcc tcatctccct
     2041 gcgccggctg ggcgaggcgg ctgtatcccc ctcgtctatg agcacaaaca tcacggtcgt
     2101 cagcacgcgg tcaacctccg ccacgtgtcg cgcgccggtc aggaacactt cgacctcgtc
     2161 gaggacggcg tcctggtcgc cgacgaacgg caggtggtcg cggcccggca gctcgacgta
     2221 cttcgcgccc gggatgtgtg aggcgacgta gcgcccgccc ccgacgggta agcaggcgtc
     2281 gcccgcgcgg tggatgacga gcgcgggcac gcggatgctc ggcagcacgt ggcgcacgtc
     2341 gatctcggcg ttcatcttcg tcagtgcgac cgccgcgccg gggctggcgc ccatccgcag
     2401 gtactcggcc caccacgcgc ggaaagaggg gtcgcccgcc gcgctcggcg cgcgctcttc
     2461 gagtccgacc gcgccgcccc agtcccgccg caactcctcc agaaaagacg cccgctcttc
     2521 ggccgtgggt gcccaagggt agtctgcggt ctgcgtccgc ttggcgtagg tgccgatcat
     2581 caccagcgcc gaggtcttct ccgggtaggt cgcggcgaag agcgccgaca tcggcccgcc
     2641 ctcggagacg ccgcagagcg ccgcgcgctc ggagccgacc gcgt
//
LOCUS       DBNGOH010000561         2874 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2831876, whole genome shotgun sequence.
ACCESSION   DBNGOH010000561 DBNGOH010000000
VERSION     DBNGOH010000561.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2874)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2874)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2874
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2831876"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            392..1942
                     /locus_tag="AC159P_10885"
     CDS             392..1942
                     /locus_tag="AC159P_10885"
                     /inference="COORDINATES: protein motif:HMM:NF052759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HIR3695996.1"
                     /translation="MAVSRKKKIIIVVAAVALLAIIVVISIFASGDDQPEVTVVKVEV
                     RPELKSTVTASGEVRPIKYINLTSEVPGRIEEISVNPGDQVTKGQALVRVDPTQLEST
                     AAAQAASVQASISDTQSARESLLAAENRVAQAQQNLAAAEASLAQARQGVVTAQTAVD
                     REQVNLNTAQRELKRTTELIESGVASRIEYDTARDRVEQAQVALRTARAQLQSANIGI
                     EEAKARVNQQRVAVRDAQIGVQSARTSVSTSESRANQQQALLRGEADRRGKATTYSPL
                     TGVVADIPARVGTFAVAGLSTTSLMTIADMSTVNVEVNVDETEITSVELGHSVKVKVD
                     AMNEREVDGVVVQKNPLAVGKSDTSGGGISNRVNVQEAKEFKVIIELKNLPDDLRSSL
                     RPGMTATATITTKTKQNVIAVPLQAIVEKAPSPTPTPVAGQAPPSPAEKPKDIKGVYV
                     LEGNKVKFVPVETGITGESDIEIVSGLPQNVEVITGPSRVLRTLKEGTVVKKQERKPG
                     ASPEEGKS"
     gene            2058..2834
                     /locus_tag="AC159P_10890"
     CDS             2058..2834
                     /locus_tag="AC159P_10890"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012499141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3695997.1"
                     /translation="MSDATLTETRTNIEPAALDHGDLLAHDAVPASLISMRDIWRTYQ
                     MGTEELHALRGVSFDVQRGEYLAIIGPSGSGKSTLMNLIGCLDTPSRGRYWINSKLVS
                     AMEDDELARIRNKEIGFVFQTFNLLARATALHNVELPLIYAGIGAAERHEKAKRALDS
                     VELSDRMTHRPNELSGGQRQRVAIARALVNHPSILLADEPTGALDSKTSHEIMNLFER
                     LHAEGNTIIVVTHEHDIAERAHRVISIRDGQIASDEKIKK"
BASE COUNT          594 a         1001 c          896 g          383 t
ORIGIN      
        1 aaatcattgt aacgcgaaac ccgacgaggc gaaaagcggt gaatagtgaa tggtcaatgg
       61 taaatagaaa aagtagcctc atccgggagg ggttccccaa cacgcgacgc acggcggcgc
      121 tcaaccgcca tttaccattc accattcact attcaccgct tctcgggggg aacgattcgc
      181 cgtttcgcgc atctaaatgg ccgaacgcgt cgtggcccgc ctccgttttc gaaaccgtca
      241 agcgcattta aacgtagaag ggtgcgtcgc ttaaccggcg gaacgtgggc tttgactcgg
      301 cggcggactg tgcgaagctg gaaacgagtc ccggcccggc gcgtagacac acacattaca
      361 caccccttaa cggaaaagga attcgagagt tatggctgtc agccgaaaga agaagattat
      421 tatcgtggtc gcggcggtcg ccctgctcgc catcatcgtc gtcatcagca ttttcgcgag
      481 cggcgacgac cagccggagg tgacggtcgt caaggtcgag gtgcgccccg agctgaaatc
      541 gacggtgacg gcctcgggcg aagtccggcc catcaagtac atcaacctga cgagcgaggt
      601 gccgggccgc atcgaggaga tttcggtcaa ccccggcgac caggtgacga aggggcaggc
      661 gctcgtgcgc gtcgatccga cgcagctcga atccaccgcc gccgcgcagg ccgccagcgt
      721 acaggcctcg atcagcgaca cgcagagcgc gcgcgaatcc ctcctggccg ccgagaaccg
      781 cgtcgcgcag gcgcagcaga acctcgccgc cgccgaggcc agcctcgcgc aggcgcggca
      841 gggcgtcgtc accgcgcaga ccgcggttga ccgcgagcag gtcaacctca acaccgcgca
      901 gcgcgagctg aagcgcacga ccgagctgat cgagtcgggc gtcgcctcgc gcatcgagta
      961 cgacacggcg cgcgaccgcg tcgagcaggc gcaggtcgcg ctccgcacgg cgcgcgcgca
     1021 gttgcagtcg gccaacatcg gcatcgagga ggcgaaggcg cgcgtcaacc agcagcgcgt
     1081 ggccgtgcgc gacgcgcaga tcggcgtcca gtcggcgcgc accagcgtct cgacgagcga
     1141 gtcgcgcgcc aaccagcagc aggcgctgtt gcgcggcgag gccgaccggc gcggcaaggc
     1201 cacgacctac tcgccgctca cgggcgtcgt cgccgacatc ccggcccgcg tcggcacgtt
     1261 cgccgtcgcg ggcctctcga ccacctcgct gatgaccatc gccgacatgt ccaccgtcaa
     1321 cgtcgaggtc aacgtggacg agaccgagat cacctcggtc gagctgggcc attccgtcaa
     1381 ggtcaaggtg gacgcgatga acgagcgcga ggtggacggc gtcgtcgtgc agaagaaccc
     1441 gctcgccgtc ggcaagagcg acacctcggg cggcggcatc agcaaccgcg tcaacgtgca
     1501 ggaggcgaaa gagttcaagg tcatcatcga gctgaagaac ctgcccgacg acctccgcag
     1561 ctcgctgcgg cccggcatga ccgcgaccgc gaccatcacg accaagacca agcagaacgt
     1621 catcgccgtg cccttgcagg ccatcgtcga gaaggcgccc agcccgacgc cgaccccggt
     1681 agccggccag gcgcccccgt cgcccgccga gaagccgaag gacatcaagg gcgtctacgt
     1741 cctcgaaggc aacaaggtca agttcgtccc ggtcgagacc ggcatcacgg gcgagtccga
     1801 catcgagatc gtcagcggtc tgccgcagaa cgtggaggtc atcaccggcc cgagccgcgt
     1861 cctgcgaacc ttgaaggaag gcaccgtcgt caagaaacag gagcgcaagc ccggcgcgtc
     1921 ccccgaagag ggcaagtctt gagggaagcg ggatgaagca aggccaagag cttccgcttc
     1981 atccctctcc ccttcgcagc aagcctgacc gctcagggag ggcgtgaacg ccaagtggaa
     2041 gctaaaggta gaagttaatg agcgacgcga cactgacgga aacacgaacg aacatcgagc
     2101 cggcggcgct cgaccacggc gacctgctcg cgcacgacgc cgtgcccgcc tccctcatct
     2161 cgatgcgcga catctggcgc acctaccaga tgggcaccga ggagctgcac gcgctgcgcg
     2221 gcgtctcgtt cgacgtgcag cgcggcgagt atctcgccat catcggcccc tccggctccg
     2281 gcaagtccac gctgatgaac ctcatcggct gcctcgacac gccctcgcgc gggcgctact
     2341 ggatcaactc caagctcgtc tcggcgatgg aggacgacga gctggcccgc atccgcaaca
     2401 aggagatcgg cttcgtcttc cagaccttca acctcttggc gcgcgccacc gcgctgcaca
     2461 acgtcgagct gccgctcatc tacgccggca tcggcgccgc cgagcggcac gagaaggcca
     2521 agcgcgcgct cgactcggtc gaactctccg accgcatgac gcaccgcccg aacgagctat
     2581 cgggcggaca gcgccagcgc gtcgctatcg cccgcgcgct cgtcaaccac ccctcgatcc
     2641 tcctggccga cgagccgacc ggcgcgctcg acagcaagac cagccacgag atcatgaacc
     2701 tcttcgagcg cctccacgcc gagggcaaca cgatcatcgt cgtcacccac gagcacgaca
     2761 tcgccgagcg cgcccaccgc gtcatctcca tccgcgacgg ccagatcgcc tccgacgaga
     2821 agatcaagaa gtagaccgcg cttcgaagtt aaacggccca cgcgagacgc ggcg
//
LOCUS       DBNGOH010000562         3823 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_82614, whole genome shotgun sequence.
ACCESSION   DBNGOH010000562 DBNGOH010000000
VERSION     DBNGOH010000562.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3823)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3823)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3823
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_82614"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            156..1733
                     /gene="aroE"
                     /locus_tag="AC159P_10895"
     CDS             156..1733
                     /gene="aroE"
                     /locus_tag="AC159P_10895"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HIR3695998.1"
                     /translation="MPWKRTARICVPVCARSAEELSRLTARAAELADFVEVRFDSLEE
                     SQLDAALDRLVADGYRRPYVFTLRPAEEGGMRAVGGDERMRFWEGLVVRNARGELNLP
                     VWADIELGLCEKWFAARDPASVGFSVICSHHDFEGVSPGLGEVFERMLRVRAYALKVA
                     VRANTITDCIEVFKMLERARAEGREMIAVAMGEAGLLTRVLGPSRGALLTFGALDEGH
                     ATAPGQTSAAELRRLYRIDAITERTVVTGLVGSPVAHSFSPHMHNAAFAARGVDAVYI
                     PFEVADAGEFARRMVRPGSREFEWNLRGFSVTAPHKTAIMRHLDWAEPAAREIGAVNT
                     VVVEGAELHGYNMDAAASLVPLLERCELRGARVAVLGAGGAARALLWSLRERGARATV
                     YARDAARARATAEGFGARLLPLDGARFEGFDAVVNPTPLGTRGERERETPATAEQLSG
                     ARVAYDLVYNPAETRFMREAREAGCDAVGGLPMLVAQAAEQFELWTGEAAPLDVMRAA
                     AATEVVRGQRSEVSENE"
     gene            1840..2856
                     /locus_tag="AC159P_10900"
     CDS             1840..2856
                     /locus_tag="AC159P_10900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008474543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HIR3695999.1"
                     /translation="MERYSRQILFEGIGEEGQRRLRGASVLVVGCGALGSAQVETLAR
                     AGVGQLFVVDRDFVEESNLQRQTMFTEADARERVPKAVAAARRVADINTDVECVAEVV
                     DVNHANVERLVSGRDIVLDGTDNFATRFLLNDACVKHGVAWVYGAAVSSYGVTMTVRP
                     RATPCLRCVFPETPAAGSAPTCDTAGVIMPIISVVAAVQVSEALKLIAGRTESLHGSL
                     MQFDVWRNEWRRVRLGEPEPDCPTCALARYETLEAEAADFTTVMCGRDAVQVSPAVPA
                     RIDLEELADRLRAAGEVKVNPYLVRLRAGAYELTIFEDARAIIRGTDDVATARSLYAR
                     YVGT"
     gene            3101..>3823
                     /locus_tag="AC159P_10905"
     CDS             3101..>3823
                     /locus_tag="AC159P_10905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696000.1"
                     /translation="MTNTKSFLRIALLAVSLVSAALVGAWPASAGGPVVWETNTRAAL
                     LAGDARGVSVTDTGALMLAPRFAQLYDTEQAFVWSSAADSAGNVYLGTGHDGKIFRVG
                     ADGRGALLYDAAELDVTALAVGRDGALYAGTSPDGKVYRVGADGKAEVYFDPADKYIW
                     SLAVLGDGSLAVGSGDTGKLYRVRSAGAKPEASLLIDTNETHIISLAADAQGNLIAGT
                     DPGGLVLRVSPEGKAFALFDSPL"
BASE COUNT          569 a         1250 c         1370 g          634 t
ORIGIN      
        1 tcggagtttg agatttcaga tgtgcgattt gaaatctcca gcctcgaatc cgaagtcgtc
       61 tcgactgcct actgcctact gtcttctgcc ctactgtttc ctcgcgtgtc ttggcgcgtc
      121 gccgccgtgt atattaatcg gcggagctga atcgaatgcc gtggaagaga acagcacgca
      181 tctgcgtccc ggtctgcgcg cggagcgcgg aagagttgtc acggttgacg gcccgcgcgg
      241 ctgagctggc ggacttcgtc gaagtgcgtt tcgactctct ggaagagtcg cagctcgacg
      301 cggcgctcga ccggctcgtc gcggacgggt accggcggcc atacgtcttc acgctccgcc
      361 cggcggaaga gggcgggatg cgcgccgtcg gcggcgacga acgcatgcgc ttctgggagg
      421 gcctcgtcgt gcgcaacgcg cggggcgagc ttaacctgcc cgtctgggcg gacatcgaac
      481 tcgggctgtg tgagaagtgg ttcgccgcga gagacccggc ctccgtcggg ttctccgtca
      541 tctgttctca tcacgacttc gaaggcgtgt cgcccggcct cggcgaagtg ttcgagcgga
      601 tgctgcgcgt gcgcgcgtac gccctgaagg tcgcggtgcg cgcgaacacg atcacggact
      661 gcatcgaggt cttcaagatg ctcgaacgcg cgcgcgcgga ggggcgcgag atgatcgccg
      721 tggcgatggg cgaggcgggt ctgttgacgc gcgtgctcgg cccctcgcgc ggcgcgctgc
      781 tcaccttcgg cgcgctcgac gaagggcacg cgaccgcgcc ggggcagacg agcgcggcgg
      841 agctgcgacg actctaccgc atcgacgcga tcaccgagcg gacggtggtg accggcctcg
      901 tcggctcgcc cgtcgcgcac tcgttctcgc cgcacatgca caacgcggcc ttcgcggcgc
      961 gcggcgtgga cgccgtctac atccccttcg aggtcgcgga cgctggcgag ttcgcgcgcc
     1021 gcatggtgcg ccccggcagc cgcgagttcg agtggaacct gcgcggcttc agcgtcaccg
     1081 cgccgcacaa gacggcgatc atgcgccacc tcgattgggc ggagcctgcg gcgcgagaga
     1141 tcggcgcggt caacacggtg gtcgtcgaag gcgcggagct tcacggctac aacatggacg
     1201 cggcggcgtc gctcgtgccg ttgcttgagc gttgcgagct gcgcggcgcg cgtgtcgcag
     1261 tcctcggcgc gggcggcgcg gcgcgcgcgt tgctctggag tctgcgcgag cggggcgcac
     1321 gggcgaccgt ctacgcgcgc gacgccgcgc gcgcgcgcgc gaccgcggag ggcttcggcg
     1381 cgcggctgct gccgctcgac ggcgcacgct tcgaaggctt cgacgcggtc gtcaacccga
     1441 cgccgctcgg cacgcgcggc gagagggagc gagagactcc cgcgaccgcc gagcaactca
     1501 gcggcgcgcg cgtcgcatat gatctcgtct acaaccctgc ggagacgcgc ttcatgcgcg
     1561 aggcccgcga ggccggctgc gatgcggtcg gcggcctccc gatgctcgtc gcgcaggccg
     1621 ccgaacagtt cgaactctgg acgggcgaag ccgcgccgct cgacgtgatg cgcgcggcag
     1681 cagcaacaga agtggtcaga ggtcagaggt cagaggtcag tgaaaacgag tgacaactcc
     1741 cgtcgcttgg aattcgaaat ctcgaatccg aagtcaaaaa aagaccgccg accgctgtcc
     1801 gccgccttct gtctttcact gacctctgac ctctgatcta tggaacgcta cagccgtcaa
     1861 attctattcg agggcatcgg cgaggagggg cagcgtcgcc ttcgcggggc gagcgtgctc
     1921 gtcgtcgggt gcggcgctct gggttcggcg caggtcgaga cgctggcgcg cgccggggtc
     1981 ggtcaactgt ttgtggttga ccgcgatttc gtcgaggagt cgaaccttca gcggcagacg
     2041 atgttcacgg aagccgatgc gcgcgagcgt gtgccgaagg ccgtcgccgc cgcgcgccgc
     2101 gtggcggata tcaacactga tgtcgagtgc gtggctgaag tcgttgatgt caatcacgcg
     2161 aacgtcgagc ggttggtcag tgggcgcgac atcgtgctcg acgggacgga caacttcgcg
     2221 acgcggttcc tgctgaacga cgcgtgcgtg aagcacggcg tcgcgtgggt ctacggcgcg
     2281 gcggtcagct cgtacggcgt gacgatgacc gtcaggccgc gcgcgacgcc gtgcctgcgc
     2341 tgcgtctttc cggagacgcc cgccgccggg agcgcgccga cgtgcgacac ggcgggcgtc
     2401 atcatgccga tcatctcggt cgtcgccgcc gtgcaggtga gcgaggcgct gaagctcatc
     2461 gcgggccgca cggagagctt gcacggttcg ctcatgcagt tcgacgtgtg gcgcaacgag
     2521 tggcggcgcg tgaggctggg cgagccggaa cccgactgcc cgacgtgcgc gctcgcgcgc
     2581 tacgagacgc tggaggcgga ggccgcggac ttcacgaccg tcatgtgcgg gcgcgacgcc
     2641 gtgcaggtct cgcccgcggt gcccgcgcgc atcgacctcg aagagctggc cgacaggctc
     2701 cgcgccgcgg gcgaggtcaa ggtcaacccg tacctcgtgc gcctgcgcgc gggcgcgtac
     2761 gagctgacga tcttcgaaga cgcccgcgcc atcatccgcg gcaccgacga cgtggcgacg
     2821 gcgcgctcgc tctatgcgcg ctacgtcgga acgtgacgat tgcggattgg ggattgcgga
     2881 ttgcggattc gtggtcgatg aaggaaccgc tatctgccct catgcaggcc gttgacgaga
     2941 gttgtgctgt cttcaatcag gctctcctct tcttccaatc cgaaatccgc attccgcatt
     3001 ccgcaatcga cggtcgccgt cccggcgggt tatgattgat caggcgatga agtcttccca
     3061 tcggcccgcc gagactctga atcacgatcc ttttctattg atgacgaaca ccaaaagttt
     3121 tctccgcatc gcactccttg cggtctctct tgtatccgcc gcgcttgtcg gcgcgtggcc
     3181 tgcgagcgcg ggcgggccgg tcgtgtggga gacgaacacg cgggcggcgc tgctggcggg
     3241 cgacgcgcgc ggcgtctcgg tgacggacac gggcgcgctc atgctcgcgc cgcgcttcgc
     3301 gcagctctac gacacagagc aggcgttcgt ctggtcgagc gccgcggact cggcgggcaa
     3361 cgtctacctc ggcacggggc acgacgggaa aatcttccgc gtcggcgcgg acgggcgcgg
     3421 cgcgctgctg tacgacgcgg cggagctgga cgtgacggcg ctcgcggtgg ggcgcgacgg
     3481 cgcgctctac gcgggcacgt cgccggacgg gaaggtctac cgcgtcggcg ccgacggcaa
     3541 ggccgaggtc tacttcgacc cggcggacaa gtacatctgg tcgctcgccg tcttgggcga
     3601 cggctcgctc gccgtcggct cgggcgacac gggcaagctc taccgcgtgc gttcggcggg
     3661 cgcgaagccc gaggcgtcgc tcctcatcga caccaacgag acgcacatca tctcgctcgc
     3721 cgcggacgcg caggggaatc tcatcgcggg cacggacccc ggcggactcg tgctgcgcgt
     3781 ctcgccggaa gggaaggcgt tcgcgctctt cgactcgccg ctg
//
LOCUS       DBNGOH010000563         4796 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3015314, whole genome shotgun sequence.
ACCESSION   DBNGOH010000563 DBNGOH010000000
VERSION     DBNGOH010000563.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4796)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4796)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4796
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3015314"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1070
                     /locus_tag="AC159P_10910"
     CDS             <1..1070
                     /locus_tag="AC159P_10910"
                     /inference="COORDINATES: protein motif:HMM:NF012770.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HIR3696001.1"
                     /translation="AVGSERAALCGVSEGGPMSALFAATYPEKTSALVMIGTYAKRVR
                     DPDYPWGPTAAEREAFFAELREHWGGAVGLEERAPSAAGDPSFRAWWAEYLRMGASPG
                     TAVALTKMNAEIDVRHVLPSIRVPALVIHRAGDECLKVEEGRYVASKIPGAKYVELEG
                     SDHLPFVGDQDAVLDEVEEFLTGVRHAHEADRVLATVLFLDIAERSARRAAMGEARWH
                     DLIRRLRAHVGKQIEWFRGREVDVAGTGALATFDGPARAVRAACAVTEYAARLGVETR
                     AGLHTGECDLVEGTQGVGGLAVEIGRAVRDRAAPGEVLVSSTVKDLVAGSGLDFEDRG
                     THTFGGVPGEWRLFRVGREKP"
     gene            1324..2151
                     /locus_tag="AC159P_10915"
     CDS             1324..2151
                     /locus_tag="AC159P_10915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020716794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR3696002.1"
                     /translation="MSSDNAKKIPPLFAVPEKEATRELFPDDGDAAPAHDAEATREAP
                     SVCPLCYGTGLEVVPGKGARRCPCRTQNAQAKLVAAARIPRRYEHCSLQNFYNVKGNS
                     YIWRAFGEAQRLIKDYYLGMDRGLLFMGPVGVGKTHLSVAILQGLMEKGVPSMFCEFG
                     SLLKQIQDSYNPVSKSSEMKVLEPIYQAEVLVLDELGASKPTDWVRDTMMQIINTRYN
                     DKKLTVFTTNFLDAHRAPGDELLEDRIGVRLRSRLYEMCKTVVIEGEDYRKRFDKQK"
     gene            complement(2260..3081)
                     /locus_tag="AC159P_10920"
     CDS             complement(2260..3081)
                     /locus_tag="AC159P_10920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3696003.1"
                     /translation="MIKKHRHPPLLWLPLLLLSPAAFAQGVPRGAEEFYNRGVERLRQ
                     KDYAGALADLDRAVALRPDHAESYFQRSFAQDGMARQEKDIAKYGDFRKAARADIDKA
                     VALAPDRAMFYKRRAAYWLTFAVEDEEKDKIIEDLTKAISLDPRDAELLFLRASTLML
                     KDDFAGALADKDRAIALAPSNAGYYGARARLKLIFLGDYEGAVADASKALGLDDGEFA
                     YETHAVRAKALLALGRKAEAETDFRKCRSLNPRCDAKLERMSELMEEMKRKRKAK"
     gene            complement(3078..3683)
                     /locus_tag="AC159P_10925"
     CDS             complement(3078..3683)
                     /locus_tag="AC159P_10925"
                     /inference="COORDINATES: protein motif:HMM:NF017496.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HIR3696004.1"
                     /translation="MEMVANPKRRYTLEEYFELERKSEERFEFRDGEVFCMSGVSEEH
                     AEIETNLIVSLKSRLRARGCRVFPANVRIKVPSAPPYRYADLSALCGEAGFEEIGGVD
                     ALTNPALIVEVLSPSTEAYDRGDKFTHYKSITSFNEYLLVAQHRPHVTHFAKQPDDSW
                     TYHEYNGLEATFKLSSLDCELTLREVHENVSFDAPSGDASS"
     gene            3854..>4796
                     /locus_tag="AC159P_10930"
     CDS             3854..>4796
                     /locus_tag="AC159P_10930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011243131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR3696005.1"
                     /translation="MPKRTDIHKILIIGSGPIVIGQACEFDYSGTQACKALMQEGFET
                     VLVNSNPATIMTDPETATRTYIEPLTVETVSAIIERERPDALLPTVGGQTALNLAMAL
                     ADAGVLDRYGVEMIGAKREAISVAEDRELYKKAMEEVGLPMPRGGFAHSWAEAEEIVE
                     TTGYPAIIRPSFTLGGTGGGTAYNPEEFEEIVRGGLAASPVNQVLIEESILGWKEFEL
                     EVMRDLADNVVIICSIENFDPMGVHTGDSITVAPAQTLTDVEYQRLRDMAITVIRKVG
                     VETGGSNIQFAVNPENGEVRVIEMNPRVSRSSALASKA"
BASE COUNT          826 a         1580 c         1557 g          833 t
ORIGIN      
        1 acgcggtcgg ctccgagcgc gcggcgctct gcggcgtctc cgagggcggg ccgatgtcgg
       61 cgctcttcgc cgcgacctac ccggagaaga cctcggcgct ggtgatgatc ggcacctacg
      121 ccaagcgcgt gcgcgacccg gattacccgt gggggccgac cgccgccgag cgcgaggcgt
      181 ttttcgcgga gttgcgcgag cactggggcg gcgcggtcgg actcgaagag cgcgcgccga
      241 gcgcggcggg cgacccctct ttccgcgcgt ggtgggccga gtacctgcgg atgggcgcca
      301 gccccggcac ggcggtcgca ctgacgaaga tgaacgccga gatcgacgtg cgccacgtgc
      361 tgccgagcat ccgcgtgccc gcgctcgtca tccaccgcgc gggcgacgag tgcctcaagg
      421 tcgaagaggg gcgctacgtg gcctcgaaga ttccgggcgc gaagtacgtc gagctggaag
      481 gctcggatca cctgccgttc gtcggcgacc aggacgccgt cctcgacgag gtcgaagagt
      541 tcctgacggg cgtccgccac gcgcacgagg ccgaccgcgt gctcgccacc gtgctcttcc
      601 tcgacatcgc agagcgttcg gcgcggcgcg ccgcgatggg cgaggcgcgc tggcacgatc
      661 tgatccgccg cctgcgcgcg cacgtcggca agcagatcga gtggttccgc gggcgcgagg
      721 tggacgtggc gggcacgggg gcgctcgcca ccttcgacgg gcctgcgcgc gcggtgcgcg
      781 ccgcctgcgc cgtcacggag tacgcggccc gcctcggtgt cgagacgcgc gccggcctgc
      841 acacgggcga gtgtgacctc gtcgagggca cgcaaggcgt gggcggcctc gccgtcgaga
      901 tcggcagggc cgtgcgcgac cgcgcggccc ccggcgaagt cctcgtctcc agcacggtca
      961 aagacctcgt cgcaggctcc ggcctcgact tcgaggatcg cggcacgcac accttcggcg
     1021 gcgtccccgg cgagtggcgc ctcttcagag tggggcgtga aaagccgtga accgtgaacc
     1081 gtgacaagtc agaaacaggt tttttaatca tcacggttta cggttcacgg ttcacggccc
     1141 ttgagttttc cacactgtgg aaatccgtaa cggggaaagt cggtcttgct ttctcaagtc
     1201 cgagtgctat aatccgcacg tttttcaagg ggcgagtttc ttacttcaag agcaggccgc
     1261 gcaacaagca ccaccccccg cgcggctcca agcacagacc gcaagccgca gccgttgagt
     1321 ttgatgtcgt cggacaacgc gaaaaagatt ccgccgctct tcgccgtgcc cgaaaaggag
     1381 gcgacgcgtg aactcttccc cgacgacggc gacgccgcgc ccgcgcacga cgccgaagcc
     1441 acgcgcgagg cgccctcggt ctgcccgctc tgctacggaa cgggactcga agtcgtgccc
     1501 ggcaagggcg cgcgccgctg cccctgccgc acgcagaatg cgcaggccaa gctcgtcgcc
     1561 gccgcacgca tcccgcgacg ctacgagcac tgctcgctcc agaactttta caacgtcaag
     1621 ggcaacagct acatctggcg cgccttcggc gaggcgcagc gcctcatcaa ggactactac
     1681 ctcggcatgg atcgcggcct gctcttcatg gggccggtcg gcgtcggcaa gacgcacctc
     1741 tccgtcgcca tcctgcaagg cttgatggag aagggcgtgc cctccatgtt ctgcgagttc
     1801 ggctcgctcc tgaaacagat acaggactcc tacaaccctg tgtcgaagtc gtcggagatg
     1861 aaggtgctgg agccgatcta tcaggccgaa gtgttggtct tggacgagct gggcgcctcg
     1921 aagccgaccg actgggtgcg cgacacgatg atgcagatca tcaacacgcg ctacaacgac
     1981 aagaagctca ccgtcttcac caccaacttc ctcgacgcgc accgcgcgcc cggcgacgag
     2041 ctgctcgaag accgcatcgg cgtccggctc cgctcgcgcc tctacgagat gtgcaagacg
     2101 gtcgtcatcg aaggcgaaga ctaccgcaag cgcttcgaca agcagaaata atttcggcga
     2161 cgaaagagcg aagacgcgat gaaggtcaac atccttcatc gcgtcttcgc tctttcgcgg
     2221 cgaacttcat atcgcctcac ggcgcttccg tcgaagcctt cacttcgcct tccgcttccg
     2281 cttcatctcc tccatcaatt cgctcatcct ctcaagcttc gcgtcgcacc gcgggttgag
     2341 gctccggcac ttgcggaagt ctgtctccgc ctccgccttg cgcccgagcg cgagcagcgc
     2401 tttggcgcgc accgcgtggg tttcgtacgc gaactcgcca tcgtcgagtc cgagggcctt
     2461 gctcgcgtcg gcgacggcgc cttcgtaatc cccgaggaag atgagcttga ggcgagcgcg
     2521 cgcgccgtaa taaccggcgt tggaaggcgc gagcgcgatg gcccggtctt tgtcggcgag
     2581 ggcgcccgcg aagtcgtcct tgagcatcag cgtgcttgcg cgcaggaaca ggagttcggc
     2641 gtcccgcggg tcgagcgaga tggctttggt caagtcctct attatcttat ccttctcttc
     2701 atcttcgacc gcgaaggtca gccagtaggc ggcccgccgc ttatagaaca tcgccctgtc
     2761 gggggcgagc gccacggcct tgtcgatgtc cgcccgcgca gctttcctga agtcgccgta
     2821 cttggcgatg tctttctcct gccgcgccat cccgtcctgc gcgaagctgc gctggaagta
     2881 gctctctgcg tggtcgggcc gcagggcgac ggcccggtcg aggtctgcca gcgcgcccgc
     2941 gtagtccttt tgcctcagcc gctcgacgcc gcggttgtag aactcctccg cgccgcgcgg
     3001 gactccctgc gcgaaagccg cgggcgagag caggagcaac ggcagccaca gcagtggggg
     3061 gtgtcggtgt ttctttatca tgaggatgcg tcgccggacg gcgcgtcgaa ggagacgttt
     3121 tcgtgaacct cgcgcagggt caactcgcag tcgagcgacg acagcttgaa ggtcgcttcg
     3181 aggccattgt actcgtgata agtccatgag tcgtccggct gctttgcgaa atgcgtgacg
     3241 tgcgggcggt gctgcgcgac cagtaggtat tcgttgaaac tcgtgatcga tttgtagtgc
     3301 gtgaacttgt cgccgcggtc gtaagcctcg gtcgaaggcg ataagacttc gacgatcagc
     3361 gcggggttgg tcaaagcgtc aacgccgccg atctcctcga acccggcctc gccgcacagg
     3421 gccgacaagt cggcatagcg atacggaggg gcgctcggca cttttatccg aacgtttgcc
     3481 gggaagactc gacagccgcg tgccctgagc cgcgatttca agctcacgat cagatttgtt
     3541 tctatttcgg cgtgctcctc gctgacgccg ctcatgcaga agacttcacc gtcccggaac
     3601 tcgaacctct cctcggactt ccgctccagc tcgaagtatt cttcgagcgt gtagcgtctt
     3661 ttcgggttgg cgaccatttc catcgtttct ctccgggcgg gacggcttaa aagtacacca
     3721 accggcggca agctcccaac gccgcggcgg gcggcggggc ggcggttcgt gctcggtcgg
     3781 ggcgttatgt ataatgccgc gaatccgttt gtaaacaatt cggcggcggt tttatccggt
     3841 gaaccaatcg taaatgccca aacgcacgga catccacaag atactcatca tcggctccgg
     3901 cccgatcgtc atcgggcagg cgtgcgagtt cgactattcg ggcacgcagg cgtgcaaggc
     3961 gctcatgcag gagggcttcg agacggtgct cgtcaactcg aacccggcga ccatcatgac
     4021 cgacccggag acggccacgc gcacctacat cgagccgctc acggtcgaga ccgtctcggc
     4081 catcatcgag cgcgaaaggc ccgacgcgct gctgccgacc gtcggcgggc agacggctct
     4141 gaatctggcg atggcactcg ccgacgcggg cgtgctcgac cggtacggcg tggaaatgat
     4201 cggcgccaag cgcgaggcga tcagcgtcgc cgaagaccgc gagctgtaca agaaggcgat
     4261 ggaggaagtg ggcttgccga tgccgcgcgg cggcttcgca cactcttggg ccgaggccga
     4321 ggagatcgtc gagacgaccg gctacccggc catcatccgc ccctcgttca cgctcggcgg
     4381 gacgggcggc ggcaccgcct acaaccccga ggagttcgag gagatcgtgc gcggcggcct
     4441 cgcggcctcg cccgtcaacc aagtcctcat cgaagagtcc atcctcggct ggaaggagtt
     4501 cgagctggag gtgatgcgcg atctggcgga caacgtcgtc atcatctgct cgatcgagaa
     4561 cttcgacccg atgggcgtcc acacgggcga ctcgatcacg gtcgccccgg cgcagacctt
     4621 gaccgacgtc gagtaccagc gcttgcgcga catggcgatc acggtgattc gcaaggtcgg
     4681 cgtcgagacc ggcggctcga acatacagtt cgccgtcaac cccgagaacg gcgaggtgcg
     4741 cgtcatcgag atgaacccgc gcgtgtcgcg ctcgtccgct ctggcgtcga aggcga
//
LOCUS       DBNGOH010000564         4551 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3383079, whole genome shotgun sequence.
ACCESSION   DBNGOH010000564 DBNGOH010000000
VERSION     DBNGOH010000564.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4551)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4551)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4551
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3383079"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..527)
                     /locus_tag="AC159P_10935"
     CDS             complement(<1..527)
                     /locus_tag="AC159P_10935"
                     /inference="COORDINATES: protein motif:HMM:NF044507.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family domain"
                     /protein_id="HIR3696006.1"
                     /translation="MSIYDDVRPLSLDGVRTYPLDERPSKVSVADFARPVEADASLRA
                     FLKGLPDILAVQSLRRLAAEVRRARALGKPIVWGVGGHVVKVGLAPLLIDLMRRGYVS
                     AVAANGSVLVHDAEVALVGSTSEDVDATLGAGAFGAADETGRLLNSAAREGADAGIGL
                     GEALGRALTSLHPKH"
     gene            626..928
                     /locus_tag="AC159P_10940"
     CDS             626..928
                     /locus_tag="AC159P_10940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696007.1"
                     /translation="MKTRAAVAAVIILLSCAPQAALAQRRPSTNEQLRELREEVRRLR
                     EEQDGLRKELQEIRRLLEQAREPARPAMPEFVSVDDDPFQGSADARVVIIDFSDYQ"
     gene            941..1432
                     /locus_tag="AC159P_10945"
                     /pseudo
     CDS             941..1432
                     /locus_tag="AC159P_10945"
                     /inference="COORDINATES: protein motif:HMM:NF024853.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
     gene            complement(1637..4495)
                     /gene="uvrA"
                     /locus_tag="AC159P_10950"
     CDS             complement(1637..4495)
                     /gene="uvrA"
                     /locus_tag="AC159P_10950"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020715019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HIR3696008.1"
                     /translation="MAIDRITVRGARQHNLKNVSVEIPRDRLTVITGLSGSGKSSLAF
                     DTIYAEGQRRYVESLSAYARQFLDQLERPDVDSVDGLSPAISIEQKTVSRSPRSTVGT
                     VTEVYDYLRLLFSSVGQPHCHVCGAQITRQSIEQIVLNVLALPEGERVMVLAPIVRGR
                     KGEFKKELEKLAKDGFLRARVDGELVSLDEEIKLDKRRNHTIEAVVDRLLIKPGVNER
                     LTESVRTALKLTGGAVLISVVDGEEKLYSERMACVNCGINVPPLEPRSFSFNSVYGAC
                     KRCHGLGTVLQVDPAKLIPEGSERADKLLFLGAADKQGSAYLKSALLAVVEHFGASPQ
                     TPFAELPKQARDAFFHGVEGQLTFRQGLYKYQNDWKGAVRWLTERMKDAPSEKVRGAL
                     EDLVSPVGCPECLGQRLQPESLAVRVGGRGIAEYTALPIEESVAAFDGLKLNDRESQI
                     AGLVLREIRNRLRFLSTVGLGYLTLDRPSATLSGGEGQRIRLATQIGSQLRGVLYVLD
                     EPSIGLHPRDNQKLLETLAALRDLGNTVLVVEHDEETIRRADYVVDLGPAAGAHGGEI
                     VASGTPEEVACNPDSVTGLYICGKRAIEVPGVRRTANGTAITIRGARHNNLKGVDAVF
                     PLGLLTVVTGVSGSGKSTLVDDILYRALAQHLYGSLEEPGAYESIEGLEHLDKVIEID
                     QSPIGRTPRSNPATYTGLFTPIRELYAMLPESRERGYKPGRFSFNVKGGRCEACEGDG
                     MKRIEMNFLPDVYVLCDVCRGARYNRETLSVKYKGKSIADLLDTTIEEAMPLLENIPQ
                     IKQKLQTLLDVGLGYIKLGQSATTLSGGEAQRIKLARELSKRATGRTVYILDEPTTGL
                     HFADVHKLLDVLQRLVSLGNTVIVTEHNLDVIKSADHIIDLGPEGGAGGGRLVAAGTP
                     EEVARTRRSHTGHALRPLLNGHTHNGNGRRKNADKKVA"
BASE COUNT          749 a         1430 c         1605 g          767 t
ORIGIN      
        1 tcgtgcttcg ggtgcagcga ggtgagcgcg cgcccgagtg cttcgccgag gccgatccct
       61 gcgtcggcgc cttcgcgcgc cgcggaattg agaagccgcc ccgtctcgtc cgccgcgccg
      121 aaggccccgg cgccgagcgt cgcgtcaacg tcctcggacg tggagccgac cagcgccacc
      181 tccgcgtcgt ggacgaggac ggagccgttg gcggcgacgg cggagacgta gccgcgccgc
      241 atgaggtcga tcaggagcgg cgcgagtccg accttgacga cgtggccgcc gacgccccag
      301 acgatgggct tgcccagggc gcgcgcgcgc cgcacctctg cggcgagccg gcggagcgac
      361 tggacggcga ggatgtcggg cagccccttg aggaacgcgc ggagcgaggc gtcggcttcg
      421 acgggccgcg cgaagtcggc gacggagact ttgctcggtc gctcgtcgag cgggtaggtg
      481 cgcacgccgt cgaggctgag cggtctcacg tcgtcgtaga tggacacttg atcttcgcgg
      541 ctccgcaaat tgactttgag ttgaccggaa ttcatcatag cgtctcgacc gagaaccggg
      601 caaacaaatc caaaggagag ttcggatgaa aacaagagca gccgtcgcgg ctgtcatcat
      661 tctactgtct tgcgccccgc aggcggcgct cgcgcagcgg cggccttcca cgaacgagca
      721 gttgcgcgag ctgcgcgagg aagtgcgccg gctgagggaa gagcaggatg ggctgcggaa
      781 ggagttgcag gagatcaggc ggctgctgga gcaggcgcgg gagcctgcga ggcccgcgat
      841 gcccgagttc gtgagcgtgg acgacgaccc gttccagggg agcgccgacg cgcgcgtcgt
      901 catcatcgac ttctcggact accagtgacc gttctgcgga agattcgtcc gtgagacgaa
      961 accacaactc gaaaaggaat acttcaacac gggcaaggtg cggtacgtct tccgcgacct
     1021 gccgctcgaa gccatccacc cgcacgcctt caaggcggcg gaggcagcca actgcgccgg
     1081 cgagcagggc aagttctggg agatgtacga acacctcttc cgcaaccagc gcgcgctcgc
     1141 gcccccgagc ctcgcgcagt acgcgcaggc gctcgcgctc gacgcggcga agttcaccgc
     1201 ctgcttcgac tcgggcaagt acgcccccga aatccgcaag gacatggccg acgcaggggc
     1261 cgccaacatc gaaggcacgc ccaacttcgt catcggcctc gccaacccga agaacccccg
     1321 cgacccacgc atcaaggtct tgaggatcat caccggcgcg cacccctacc agaccttcaa
     1381 ggccgtgctc gacgccgcgc tggccgtcgc cgccgccgcc gagcagaagt aagacggaag
     1441 gggaaaaggg aaaaaggtta ataaggggaa aagggaaaga gggaagataa gaaacagcgt
     1501 gcctctcatc ttctttaact gaagatgaga ggcacgctgt ttcttatcac tcgaaggagg
     1561 atgagaagca cgctgcttct catcctcctt cttccctttt cgtctcttcc ccttttaacc
     1621 ttttcgccct tcttcctcag gcgactttct tgtcggcgtt cttgcggcgc ccgttgccgt
     1681 tgtgtgtgtg gccgttgagg agggggcgca gcgcgtggcc ggtgtgcgag cggcgtgtgc
     1741 gggcgacctc ttcgggggtg ccggcggcga cgaggcgacc gccacccgcg ccgccttcgg
     1801 ggccgaggtc gatgatgtgg tcggcggact tgatgacatc gaggttgtgt tcggtgacga
     1861 tgacggtgtt gccgaggctg acgaggcgtt gcagcacgtc gagcagcttg tgcacgtctg
     1921 cgaagtgcag gcccgtggtc ggctcgtcga ggatgtagac ggtgcggccc gtggcgcgct
     1981 tcgagagttc gcgcgcgagc ttgatgcgct gcgcctcgcc gcccgagagc gtcgtggccg
     2041 actggccgag cttgatatag ccgagtccca cgtcgagtaa ggtttggagc ttctgcttga
     2101 tctgcgggat gttctctaag agcggcatcg cctcctcgat ggtcgtgtcg aggaggtcgg
     2161 cgatggactt gcccttgtac ttgacggaga gcgtttcgcg gttgtagcgc gcgccgcggc
     2221 acacgtcgca gaggacgtac acgtcgggga ggaagttcat ctcgatgcgc ttcatgccgt
     2281 cgccctcgca cgcctcgcag cgcccgccct tgacgttgaa ggagaagcgg cccggcttgt
     2341 agccgcgctc gcgcgattcg gggagcatcg cgtacagctc gcggatgggc gtgaagaggc
     2401 cggtgtaggt cgccgggttc gagcgcggcg tgcgaccgat gggcgactgg tcgatctcga
     2461 tgaccttgtc gaggtgttcg agtccttcga tggattcgta ggcgcccggc tcttccagcg
     2521 agccgtagag gtgctgcgcg agcgcgcgat agaggatgtc gtccacgagc gtcgatttgc
     2581 cggagccgga gacgccggtg acgaccgtga gcagcccgag cgggaagacg gcgtccacgc
     2641 ccttgaggtt gttgtggcgt gcgccgcgga tggtgatggc cgtgccgttg gcggtgcgcc
     2701 gcacgcctgg gacttcgatg gcgcgcttgc cgcagatgta gaggccggtg acggagtcgg
     2761 ggttgcaggc gacttcttcg ggcgttcccg aagcgacgat ctcgccgccg tgcgcgcccg
     2821 ccgcggggcc gaggtcaacg acgtagtcgg cgcggcggat cgtctcctcg tcgtgctcga
     2881 cgacgagcac ggtattgccg aggtcgcgta gcgccgccag cgtttcgagc agcttctggt
     2941 tgtcgcgcgg gtgcagaccg atggacggct cgtccagcac gtagagcacg ccgcgcagtt
     3001 gcgagccgat ctgcgtcgcc agacggatgc gctggccttc gccgccggag agcgtggccg
     3061 aggggcggtc gagcgtgagg tagccgaggc cgaccgtcga gaggaagcgc aggcggttgc
     3121 gtatctcgcg caggacgagg ccggcgatct gcgactcgcg gtcgttgagc ttcagcccgt
     3181 cgaaggcggc gaccgattct tcgatgggga gcgccgtgta ctccgcgatg ccgcgcccgc
     3241 cgacgcgcac cgcgagcgat tcgggttgca ggcgctggcc gaggcactcg gggcacccga
     3301 ccggcgagac gaggtcttcc agcgccccgc gcaccttctc gctcggcgcg tccttcatgc
     3361 gctcggtgag ccagcgcacg gcacccttcc agtcgttctg gtatttgtag agaccctggc
     3421 ggaaagtgag ttgaccctcg acgccgtgga agaaggcgtc gcgcgcctgc ttcggcagct
     3481 cggcgaaggg cgtctgcggc gaggcgccga agtgctcgac gacggccagc agcgcgctct
     3541 tcaaataggc cgaaccctgc ttgtccgccg cgccgaggaa gagcagcttg tccgcgcgtt
     3601 ccgagccttc cgggatgagc ttcgccggat cgacctgcaa gactgtgccg agtccgtggc
     3661 agcgcttgca cgcgccgtag accgagttga aggagaacga gcgcggctcc aagggcggga
     3721 cgttgatgcc gcagttcacg caggccatgc gctcggaata gagcttctcc tcgccgtcca
     3781 cgaccgagat gagcactgcg ccgcccgtca gcttcagcgc cgtgcggacg gactcggtca
     3841 aacgctcgtt gacgccgggc ttgatgagca ggcggtcaac caccgcttcg atggtgtggt
     3901 tgcgccgctt gtcgagcttg atctcctcgt cgagcgagac cagctcgccg tccacgcgcg
     3961 cgcgcaggaa gccgtccttc gccagcttct caagctcctt cttgaactcg cccttccggc
     4021 cccgcacgat gggcgcgagc accatcacgc gctcgccctc ggggagcgcg agcacgttca
     4081 gcacgatctg ctcgatggac tggcgcgtga tctgcgcgcc gcagacgtgg cagtgcggct
     4141 ggccgaccga agagaacagg aggcgcaggt agtcgtagac ttccgtcacc gtccccacgg
     4201 tcgagcgcgg gctgcgcgag acggtcttct gctcgatgga gatggccggg gagaggccgt
     4261 ccacagagtc cacgtccggg cgctccaact ggtcgaggaa ctggcgcgcg taggccgaca
     4321 gagactcgac gtagcggcgc tggccctcgg cgtagatcgt gtcgaaggcg agcgaagact
     4381 tgcccgagcc ggagagtccg gtgatgaccg tcaggcggtc gcgggggatt tcgacgctga
     4441 cgttcttcag gttgtgctgg cgcgcgccgc gcaccgtgat gcggtcaata gccatcgact
     4501 aaaacaaccc gtgcccctca ttgtgagctc ccgcgcatgg tgcggaaacg g
//
LOCUS       DBNGOH010000565         3118 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4115616, whole genome shotgun sequence.
ACCESSION   DBNGOH010000565 DBNGOH010000000
VERSION     DBNGOH010000565.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3118)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3118)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3118
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4115616"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="AC159P_10955"
     CDS             <1..222
                     /locus_tag="AC159P_10955"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HIR3696009.1"
                     /translation="VHLECRFLRIIELDAPFTLRFTVGKLGRTSIRYDYQVFDAENQL
                     AIEGTMTVVVLHDGKPTEIPPALRAALGG"
     gene            complement(401..1510)
                     /locus_tag="AC159P_10960"
     CDS             complement(401..1510)
                     /locus_tag="AC159P_10960"
                     /inference="COORDINATES: protein motif:HMM:NF012335.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent alcohol dehydrogenase"
                     /protein_id="HIR3696010.1"
                     /translation="MNIGAAILWEAGAPLSVERAELGAPRAGEVLVELKAAGVCHSDL
                     HPARGEWPVRTPLVLGHEGAGVVREVGAGVERVREGDHVVFCWAPPCGSCPPCMEGRP
                     VLCDRLERTTYRNRLPAGDTRLSARGQALAHYNGTACFADFAVVAEEGVIPVPRDVPF
                     EALATLGCAVVTGVGAVLNAARAEAGAKVLVIGVGGVGLNVVQGARLAGCERIIALDT
                     RSEPLRLACEFGATDRIAASDENVAEQVKELTDGRGADYVFDTVGAPATLAQALACAR
                     KGGTVVVTGLSRLDARADIKLFPFVMQEKRLVGSLYGSGQPARDVPRLVELYREGRLK
                     LAELATRTYRLEAVNDALAALASGEGARGIIRWEQ"
     gene            complement(1532..2038)
                     /locus_tag="AC159P_10965"
     CDS             complement(1532..2038)
                     /locus_tag="AC159P_10965"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015350983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0047617 - fatty acyl-CoA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HIR3696011.1"
                     /translation="MSSTDERTERTHTVSWEDPRALAEAARGLTGLEFLRKIVAGELP
                     RPPISALLSFGVAEVGEGRAVFTAEPAEYHYNPLGVVHGGLAATLLDSAMGCAVHTTL
                     PAGAGYTTLEIKVNYVRPLTAETGGVRCEARVIHVGGRTATAEGRVVDGDGKLYAHGS
                     TTCMVFRP"
     gene            complement(2077..2550)
                     /locus_tag="AC159P_10970"
     CDS             complement(2077..2550)
                     /locus_tag="AC159P_10970"
                     /inference="COORDINATES: protein motif:HMM:NF024273.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HIR3696012.1"
                     /translation="MREVVRIGSQLKRAFEGGAWHGSSLRELLADVSAVQAAARPLDG
                     AHSIWEIVLHIAAWQGFVTRALGGEPMPSNLPEEENWPPIGNASEAAWRDTARSLGEG
                     NKRLRDAVRKLVEEDLDRIVAAREYSVHFMLHGVLQHSLYHAGQIALLKKATGQR"
     gene            complement(2580..3035)
                     /locus_tag="AC159P_10975"
     CDS             complement(2580..3035)
                     /locus_tag="AC159P_10975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HIR3696013.1"
                     /translation="MKEIESVAKLWELVGQEVGAGEWVEVSQARIDRFAAATEDRQWI
                     HTDAERAARESPFKGTIAHGFLTLSLLSELLRTAVSVGGVRMGVNYGLNRVRFVAPVP
                     AGSRLRGRFVLTALEEIRGGVEAAWGVTVEREGRDKPCCVAEWLVRYYV"
BASE COUNT          504 a         1185 c          935 g          494 t
ORIGIN      
        1 gtccacctcg aatgccgctt cctccgcatc atcgaactcg acgccccctt cacgctgcgc
       61 ttcaccgtcg gcaaactcgg gcgcacctcc atccgctacg actatcaggt cttcgacgcc
      121 gaaaaccaat tggcgattga aggcaccatg accgtcgtcg tcctccacga cggcaagccg
      181 accgagatac cgcccgccct gcgcgccgcg ctcggcggct gaagcagcgg cagtaggcag
      241 taggcagtag gcggtaggca gtaagacaaa agccggaggc ttgagattca acatctcatg
      301 cctccggctt tcctgcttga agagcgagag agctttgcac ttcaacgctc aatcgtcttc
      361 gctcttctgt cttgactgcc taccgcctac tgcctactgc ttactgctcc cagcggatga
      421 tgccgcgcgc gccttcgccg cttgcgagtg cggcgagggc gtcattgacc gcttcgaggc
      481 ggtaggtgcg cgtggcgagt tcggcgagct tcagacggcc ctccctgtag agttcgacga
      541 gacgcggaac gtctctggcg ggttggcccg agccgtagag cgagccgacc agacgcttct
      601 cctgcatgac gaacgggaag agcttgatgt cggcgcgcgc gtcgaggcgc gagaggccgg
      661 tcacgaccac cgtcccccct ttgcgcgcgc aggcgagcgc ctgtgcgagt gtcgcggggg
      721 cgcccaccgt gtcgaagacg taatccgccc cgcgcccgtc cgtcaactct ttcacctgct
      781 ccgccacgtt ctcgtcggac gccgctatcc ggtcggtcgc gccgaactcg cacgcgagcc
      841 gcagcggctc actccgcgtg tccaacgcga tgatcctttc gcacccggcg agccgcgcgc
      901 cctgcacgac gttcaggccg acgcccccta cgcctatcac caatactttc gcgcccgcct
      961 ccgcgcgcgc cgcgttcagc accgcgccga cgcccgtcac gaccgcgcac ccgagcgtcg
     1021 ccagcgcctc gaacggcaca tcccgcggga cgggtatcac gccctcttcg gccaccaccg
     1081 cgaagtccgc gaagcaggcc gtgccgttgt agtgggccaa cgcctgcccc cgcgcgctca
     1141 gccgcgtgtc gcccgccggc aagcggtttc gataagtcgt ccgttccagc ctgtcgcaga
     1201 ggacggggcg gccctccatg cacggcgggc acgagccgca cggcggcgcc caacagaaaa
     1261 ctacgtgatc gccctcgcgc actcgctcca cgcccgcgcc gacctcgcgc acgacgcccg
     1321 cgccctcgtg cccgagcacg agcggcgtcc gcacgggcca ctcgccgcgc gcagggtgca
     1381 ggtcgctgtg acagacgccc gcggccttca gctcgaccag cacttcgccc gcgcgcggcg
     1441 cacccagttc cgcccgctcg accgagagcg gcgcgcccgc ctcccacaaa atcgccgcgc
     1501 cgatgttcat ccgggtagat accctccggg ttcacggacg aaagaccatg caggtcgtgg
     1561 agccgtgcgc gtagagcttc ccgtcgccgt ccacgacgcg cccctcggcg gtcgccgtgc
     1621 gcccgccgac gtgaatcaca cgcgcctcgc agcgcacccc gcccgtctcc gccgtcagtg
     1681 gacgcacgta gttgaccttg atctcaagcg tcgtgtagcc cgcgcccgcg ggcaacgtcg
     1741 tgtggacggc gcagcccatc gccgagtcga gcagcgtcgc cgccagcccg ccgtgcacga
     1801 cgccgagggg gttgtagtga tactccgccg gctcagccgt gaagaccgcg cggccctcgc
     1861 ccacctccgc gacgccgaag ctcagcagcg cgctgatggg cgggcgcggc aactcgcccg
     1921 cgactatctt gcgcaggaat tcgagacccg tgagaccgcg cgcggcctcc gcgagagcgc
     1981 gcgggtcttc ccaactgacc gtgtgcgtgc gctccgttcg ttcgtcggtg ctgctcataa
     2041 atgtgctgtc gtccagccgc gcgctttgtc ggcggcttaa cgttgacccg tcgctttctt
     2101 cagcaaggct atctggcccg cgtgatagag gctgtgttga aggacgccgt ggagcatgaa
     2161 gtggacggaa tactcgcgcg ccgccacgat cctgtccaaa tcctcttcaa cgagtttgcg
     2221 caccgcatcg cgcagacgct tgttgccttc accgagactc cgcgccgtgt cgcgccacgc
     2281 cgcctcggac gcgttcccga tgggcggcca gttctcttcc tcggggaggt tcgaaggcat
     2341 cggctcgcct ccaagcgcgc gcgtcacgaa gccctgccac gccgcgatgt ggaggacgat
     2401 ttcccaaatg ctgtgcgcgc cgtcgagcgg cctcgcagcg gcctgcaccg ccgacacgtc
     2461 cgcgagaagc tctcgcagcg acgagccgtg ccacgccccg ccctcgaagg cgcgcttcag
     2521 ttggctcccg attctgacca cctctcgcat acgacctccg aaaacttttc gcgccgctct
     2581 cagacgtaat aacgcaccag ccactcggcc acgcagcaag gcttgtcgcg gccctcgcgc
     2641 tcgaccgtca cgccccacgc ggcctcgacg ccgcccctta tctcttccaa agcggtgagc
     2701 acgaagcgac cgcgcaggcg cgacccggcg ggcaccggcg cgacgaagcg cacgcggttc
     2761 aaaccgtagt tgacgcccat gcgcacgccg cccacggaca cagccgtccg caacaactcg
     2821 ctcaacagag agagcgtcaa aaagccgtgc gctatcgtcc ctttgaaggg cgactcgcgg
     2881 gcggcgcgct ccgcgtccgt gtgtatccac tgccggtctt cggtcgccgc cgcgaaccgg
     2941 tctattcgcg cctgcgacac ctcgacccac tcgcccgcgc ccacctcctg cccgacgagt
     3001 tcccacagct tcgccacgct ttcgatctcc ttcaaggcgt ctttcctcac acaccgacgt
     3061 tagctgacca aaacgaataa cacagcgggc gcagaggcgc ggagggcgcg gagaggaa
//
LOCUS       DBNGOH010000566         2318 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4482217, whole genome shotgun sequence.
ACCESSION   DBNGOH010000566 DBNGOH010000000
VERSION     DBNGOH010000566.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2318)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2318)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2318
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4482217"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..466
                     /locus_tag="AC159P_10980"
     CDS             <1..466
                     /locus_tag="AC159P_10980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HIR3696014.1"
                     /translation="VEVPEACEFSAGLKRRALEVYEAGPVRWDEDEMRLRRYRLTDWV
                     DDIRHPRAAEELVATGASLYQDVADFFLLSRGLWAAHSKTIPRRLREVDAEFAERFRR
                     AFEALFAEKRQAPAIALVEEMLGPHGGLLFDGFKRNARPEERKSPNAEDATS"
     gene            612..1829
                     /locus_tag="AC159P_10985"
     CDS             612..1829
                     /locus_tag="AC159P_10985"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF015418.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HIR3696015.1"
                     /translation="MISVAEAVRIVLEQSSPLDAERVPLGEALGRVLAEDVLADSDLP
                     PFDRAQMDGYAVRSEDLREVPARLRVVGEAAAGRGWRGSLGAGEAVRIMTGAPLPVGA
                     DSVQQVEVTRELEGGTLVEVARATEPGQFFVPRASEIRKGETVLRAGDEVNAARMAAL
                     ASFGYASVAVRRRPRVAVLATGTELVPVEEQPGRDQIRDSNSYSLAAYAALAGAHVER
                     LPFAGDDEGLLMRSISEAAERADVIVLSGGVSMGAYDFTKAALRSLGAEVFFERVSLR
                     PGKPTVFARLRGARPVLAFGLPGNPVSVRVTFNLFARTALRAMQGDTRPALAEERAVL
                     ARDAKGSAERASYLPATLSTDDAGRLVAKPLKWGGSSDFVAFARATALVVVPAGVGVA
                     EAGSVVGVVRLPG"
     gene            1836..2318
                     /gene="moaC"
                     /locus_tag="AC159P_10990"
     CDS             1836..2318
                     /gene="moaC"
                     /locus_tag="AC159P_10990"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011646563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061799 - cyclic pyranopterin
                     monophosphate synthase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HIR3696016.1"
                     /translation="MDKLSHVDEEGRAAMVDVGAKQPSARRAVASARVLMSRETVEAV
                     RAHRTPKGDPLEVARLAGLMAAKRTADLIPLCHPLALTHIEVRAELTDDGVRLEAEAA
                     TTAQTGVEMEALTAASVAALTVYDMCKAIEKGIVITDLRLEEKTGGKSGDWKRESREP
                     "
BASE COUNT          324 a          781 c          869 g          344 t
ORIGIN      
        1 cgtcgaggtg ccggaggcgt gcgagttctc tgcggggttg aaacggcgcg cgctggaagt
       61 ctacgaggcg gggccggtgc gttgggacga ggacgagatg cgtctgaggc gctaccgttt
      121 gacggactgg gtggacgaca tcaggcaccc gcgcgcggcg gaagaactcg tcgccacggg
      181 cgcgtctctg tatcaggacg tggccgactt cttcctgctc tcgcgcggcc tgtgggcggc
      241 gcacagcaag acgatcccgc ggcggctgcg cgaagtggac gccgagttcg cggagcgttt
      301 ccgccgcgcg ttcgaggcgc tgttcgcgga gaagcggcag gcgccggcca tcgcgctcgt
      361 cgaggagatg ctcgggccgc acggcggcct gctcttcgac ggcttcaaaa gaaacgcgcg
      421 gcccgaagaa cgcaaatcgc cgaacgcgga agacgcgacc tcctgaacac gccgccgagg
      481 gaagatgaga ggcacgctcc ttccccgtcc gtaagtgctt cgtctgcgtc gctcccgtcg
      541 tgctccgtcc gcgttgctcc cgcttcatcc gtgcgctttg aactttgagt ttcgaacttt
      601 aaactcgacg catgatttcg gtcgccgaag ccgtccgcat cgtcctcgaa caatcgtcgc
      661 cgctcgacgc ggagcgcgtc ccgctcggcg aggcgctcgg gcgcgtgctc gcggaggacg
      721 tgttggccga cagcgacctg ccgccgttcg accgcgcgca gatggacggg tacgcggtgc
      781 gttcggagga cttgcgcgag gtgcccgcgc ggctgcgcgt ggtgggcgag gcggcggcgg
      841 ggcgcggctg gcgcggctcg ctcggggcgg gcgaggccgt gcggatcatg acgggggcgc
      901 ctttgcctgt gggcgcggac tcggtgcagc aggtcgaggt gacgcgcgag cttgagggcg
      961 gcacgttggt cgaagtcgcg cgagcgacgg agccgggcca gttcttcgtc ccgcgcgcga
     1021 gcgagatcag gaagggcgag accgtgttgc gcgcgggcga cgaggtgaac gcggcgcgca
     1081 tggcggcgct cgcgtcgttc ggctacgcgt cggtcgccgt gcgccgtcgc ccgcgcgtgg
     1141 ccgtgctggc gacgggcacc gagcttgtgc ccgtcgaaga gcagccggga cgcgaccaga
     1201 tacgcgactc gaacagctac tcgctcgcgg cctacgccgc gctcgcgggc gcgcacgtcg
     1261 agcggctgcc cttcgcgggc gacgacgagg gactgctgat gcgttcgatc tcggaagcgg
     1321 cggagcgcgc ggacgtgatt gtgctgtcgg gcggcgtctc gatgggcgct tacgatttca
     1381 cgaaggccgc gctgcgctcg ctcggcgcgg aggtcttctt cgagcgcgtc tctctgaggc
     1441 cgggcaagcc gaccgtcttc gcgcgtctca ggggcgcgcg gcccgtgctc gccttcggcc
     1501 ttccgggcaa ccccgtctcc gtccgcgtca cgttcaacct gttcgcgcgc accgcccttc
     1561 gcgcgatgca gggcgacacg cggcccgcgc tcgcggagga gcgcgccgtg ctcgcgcgcg
     1621 acgcgaaagg ctccgccgag agggcgagct acctgcccgc gaccttgagc acggacgacg
     1681 cgggccgcct cgtcgcgaag cccctgaagt ggggcggctc ttcggacttc gtcgccttcg
     1741 cgcgcgccac cgcgctcgtc gtggtgcccg cgggtgtggg cgtggcagag gccgggagcg
     1801 tggtcggcgt cgtccgtctg ccggggtgag aacttatgga caagctgtcg cacgttgacg
     1861 aagagggccg cgccgcgatg gtcgatgtgg gcgcgaagca gccgagcgcg cggcgcgccg
     1921 tcgcctccgc gcgcgtcctg atgtcgcggg agacggtcga ggccgtgcgc gcgcaccgca
     1981 cgccgaaggg cgacccgctc gaagtcgcgc gcctcgcggg gctgatggcc gccaagcgca
     2041 ccgccgacct gatcccgctc tgccacccgc tcgcgctcac gcacatcgag gtgcgcgccg
     2101 aactcaccga cgacggcgtc cgcctcgaag ccgaggccgc gaccaccgcg cagaccggcg
     2161 tcgagatgga agcgctcacc gccgcctcgg tcgccgcgct caccgtctac gacatgtgca
     2221 aggccatcga gaaaggcatc gtcatcaccg acctgcgact cgaagagaag acgggcggca
     2281 agtccggcga ctggaaacgc gaaagccgtg aaccgtga
//
LOCUS       DBNGOH010000567         2806 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1367802, whole genome shotgun sequence.
ACCESSION   DBNGOH010000567 DBNGOH010000000
VERSION     DBNGOH010000567.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2806)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2806)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2806
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1367802"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            160..>2806
                     /locus_tag="AC159P_10995"
     CDS             160..>2806
                     /locus_tag="AC159P_10995"
                     /inference="COORDINATES: protein motif:HMM:NF025141.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HIR3696017.1"
                     /translation="MPQRSLFSLPLLFVVGATLVALAAASGKGVSPPPPANDGATAGS
                     LQIIGAGGTAGAFCPLKHTDVKAEVSGFLSRVTVTQRFENSSAEKIEAVYAFPLPRAA
                     AVDDMTLKVGERTVRGKVMRREEAQAVYAGARAAGQVAGLLDQERPNVFTQSVANIMP
                     GEKVTVTISYVETLKYEDGTYEFSFPAVVGPRYVPGVPTEQQPKGNGFAPDTDKVPDA
                     SRITPHPAPAGVRAGHDLSIEVALDAGLPVESLQSPTHEVDVERAGGERARVRLKNRA
                     TIPNKDFVFRYDVAGRRIGDALLTHRGVRGGYFTLVLQPPERVAPEDVTPKELVFVLD
                     TSGSMQGFPLEKAKEAMGHALDGLNPQDTFNLITFAGDTHVLFPQPVPATKENLRRAQ
                     AFLASRSGAGGTEMMKAVRAALAPSGAQAHVRVVCFMTDGYVGNEFEIINEVQKHPNA
                     RVFSFGIGSSVNRFLLDKMAEEGRGEVEYVSLGDDGSAAARRFHERVRNPLLTDISVD
                     WGGLPVADVYPRRIPDLFGAKPVVLSGRFEGGGRGLVRLKGRAGVNHFEREITVELPE
                     SQPEHDVLATLWARRRVEDLMSMDYAGAQRGNMVDDVREAVTRLGIDYRLMTQFTSFV
                     AVEEMTVTDGGGPPRRIDVPVELPEGVSREGVFGRSNDPRGFGFQFYAGRRHYAGGGG
                     AGPMPALGLIGPGPVVSGGVLNGKAVSKPAPQYPAVAKAARATGTVSVLVTVDEGGKV
                     VAASPVSGHALLTKAAVDAARQARFAPTLLSGKPVKTTGIITYNFALDGATGAATVTT
                     GSDSVEMTLPETEEQKLRRALDKLHPSLAAIVERLKDKSAPPTADEARLVRDGKAEVH
                     VWLTEQSPEVVGQLKRLGFEVVLEPK"
BASE COUNT          441 a          957 c         1014 g          394 t
ORIGIN      
        1 gaaccggggg cggaaaaaat ggaaccgcaa gcagcaccca cggtgtctat gtcaacgacc
       61 ttcaagcagc ccgtgtcgcg cctcgcgttc gcgcacgtct ctcgccgtgt gcagaggaga
      121 aaatcgatga tgcgtcggaa cccgcctgtg tctttcctgt tgcctcagag atcgttgttc
      181 tcgctgccgc tgttgttcgt cgtcggcgcg actctggtgg cgctcgccgc cgcgtccggg
      241 aagggcgtgt cgccgccgcc gcccgcgaac gacggcgcga ccgcaggctc gctacagatc
      301 atcggcgcag ggggcacggc gggcgctttc tgcccgctca agcacacgga cgtgaaagcg
      361 gaggtgagcg gctttctgtc gcgcgtcacc gtgacgcagc ggttcgagaa ttcttcggcg
      421 gagaagatcg aggccgtcta cgccttcccg ctgccgcggg ccgccgccgt cgatgacatg
      481 acgctgaagg tcggcgagcg gacggtgcgc ggcaaggtga tgcggcgcga agaggcgcag
      541 gcggtctacg cgggcgcgcg cgcggcgggg caggtcgcgg gcctgctgga tcaggagcgc
      601 ccgaacgtct tcacgcagtc ggtcgccaac atcatgccgg gcgagaaggt gacggtgacg
      661 atcagctacg tcgagacgct gaagtacgaa gacggcacct acgagttctc gttcccggcg
      721 gtggtcggcc cgcgctacgt gccgggcgtg cccaccgaac agcagccgaa aggcaacggc
      781 ttcgcgcccg acacggacaa ggtgccggac gcctcgcgca tcacgccgca ccccgcgccc
      841 gcgggcgtgc gcgccggcca cgacctctcc atcgaggtcg cgctcgacgc ggggctgccc
      901 gtcgagtcgt tgcagtcgcc gacgcacgag gtggacgtgg agcgcgcggg cggcgagcgt
      961 gcgcgcgtgc ggctgaagaa ccgcgcgacg ataccgaaca aggacttcgt cttccgctac
     1021 gacgtggcgg gccgccgcat cggcgacgcg ctgctcacgc accgcggcgt gcgcggcggc
     1081 tacttcacgc tcgtcctgca accgcccgag cgcgtcgcgc cggaggacgt gacgccgaag
     1141 gagctggtct tcgtgctcga cacctccggc tcgatgcagg gcttccccct cgagaaggcg
     1201 aaggaggcga tgggccacgc gctcgacggc ctcaatccgc aggacacgtt caatctcatc
     1261 accttcgcgg gcgacacgca cgtcctcttc ccgcagcccg tgcccgcgac gaaagagaac
     1321 ctccgcaggg cgcaggcgtt cctcgcgtcg cgaagcggcg cgggcggcac cgagatgatg
     1381 aaggcggtgc gggcggcgct cgcgccttcc ggcgcgcagg cgcacgtccg cgtcgtctgc
     1441 ttcatgaccg acggctacgt cggcaacgag ttcgagatca tcaacgaggt gcagaagcac
     1501 ccgaacgcgc gcgtcttctc gttcggcatc ggctcttcgg tcaaccgctt cctgctggac
     1561 aagatggccg aggaggggcg cggcgaggtc gagtacgtct cgctcggcga cgacggctcg
     1621 gcggcggcgc gccgcttcca cgagcgcgtg cgcaaccctt tgctcacgga catctccgtc
     1681 gactggggcg ggctgccagt cgccgacgtg tacccgcggc gcatccccga cctgttcggc
     1741 gccaagccgg tcgtgctgtc gggccgcttc gagggcggcg ggcgcggtct agtccgcctg
     1801 aaggggcgcg cgggcgtcaa ccacttcgag cgcgagatca cggtcgagct gccggagtcg
     1861 cagcccgagc acgacgtgct cgccacgctc tgggcgcggc gccgcgtcga agacctgatg
     1921 agcatggact acgcaggggc gcaacgcggc aacatggttg atgacgtgcg cgaggccgtg
     1981 acgcggctcg gcattgatta ccgactgatg acgcagttca cttcgttcgt cgccgtcgag
     2041 gagatgaccg tcacggacgg cggcggcccg ccgcgccgca tcgacgttcc cgtagagctg
     2101 cccgaaggcg tcagccgcga aggggtgttc ggcaggtcca acgacccgcg cggcttcggc
     2161 tttcagttct acgccggtag gcggcactac gccggggggg gcggcgcggg gccgatgccc
     2221 gcgctggggc tgatcggccc cggcccggtc gtgtccggcg gcgtgctcaa tggcaaggcc
     2281 gtcagcaagc ccgcgccgca gtaccccgcg gtggcgaagg ccgcgcgcgc gaccgggacg
     2341 gtgagcgtgc tggtgacggt cgacgagggc gggaaggtcg tcgcggccag ccccgtctcg
     2401 gggcacgcgc tcctgacgaa ggccgccgtc gacgcggccc ggcaggcgcg gttcgcgccg
     2461 acgctgctct ccggcaagcc cgtgaagacg acgggcatca tcacctacaa cttcgcgctc
     2521 gacggcgcga cgggcgccgc cacggtcacg accggcagcg acagcgtcga gatgacgctg
     2581 cccgagaccg aagagcagaa gctccggcgg gcgctcgata agctgcaccc gtcgctcgcc
     2641 gccatcgtcg agcgcttgaa ggacaagagc gcgccgccga cagccgacga ggcgaggctc
     2701 gtccgcgacg gcaaggcgga agtccacgtg tggctgacgg agcaatcgcc ggaggtcgtc
     2761 gggcagctca agcggctcgg cttcgaagtc gtcctcgaac cgaaga
//
LOCUS       DBNGOH010000568         3037 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1185133, whole genome shotgun sequence.
ACCESSION   DBNGOH010000568 DBNGOH010000000
VERSION     DBNGOH010000568.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3037)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3037)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3037
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1185133"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..263)
                     /locus_tag="AC159P_11000"
     CDS             complement(<1..263)
                     /locus_tag="AC159P_11000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HIR3696018.1"
                     /translation="MNKSFWRVGLLYVAAIVVAGAGPLDASAAANRGSTRRPYGVITG
                     TVLDGRGQPLAGALVKVLRDGAGQIVSESRSAADGSFIARVAPG"
     gene            703..1995
                     /locus_tag="AC159P_11005"
     CDS             703..1995
                     /locus_tag="AC159P_11005"
                     /inference="COORDINATES: protein motif:HMM:NF017044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HIR3696019.1"
                     /translation="MTELVRATRLPTGLTVLTEFMPSLRSVTVGVWVRRGSRHERPEM
                     NGICHFIEHALFKGTRRRTALDIAVESDRLGGHFDAYTTHELTGFAMKVVDTALPQAF
                     DLLSDMLARPRFDEEDLERERRVIIEEMKMVEDTPDEYLIELFSAEYFPGHSLGRPIE
                     GTEETVSSFDRERTARYHASAYAPRNLVVAAAGNVEHDELVELAARVFDERLAEGARA
                     ESEADEGEPEVPPVHAAPILIRRKKELEQAHLILASPWPSSRDEDRYAASLLGSVIGG
                     GTSSRLWQRVREERGLAYSVGAAASQFTDAGVFQIYAGTSPEHLDEVLDLSMEELRRV
                     ARERVGEDELRLVKDQAVSSILLSLESTSARAGALARQEIVHGRRIAPDEVIARYEAV
                     TPDDLLRLARQFFRGDALALGALGDLNGFRVERSRLEI"
     gene            2123..2944
                     /locus_tag="AC159P_11010"
     CDS             2123..2944
                     /locus_tag="AC159P_11010"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024118.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3696020.1"
                     /translation="MRLTVLGSGSTGNAVLICAGETRVLVDAGMSARETARRLALVGE
                     DAARLDGVIITHEHGDHAGGLRVLLKSLECPVYISGATRDAYIREKSRHNDEPRRRAE
                     VLRDRTEEISSSEDFRIGEIDFHPFTIPHDAADNFGFVATHAGVRVATLMDFGHVTTL
                     LAERLRGCAAIVIESNHSRDMLKACDVYPWELKQRILSRTGHLSNEDVAAWLRDGFDG
                     AADHVVLAHLSQRANNPHVAKLTAEMALRERGGLFRTTTEVTLSHHKEPTSWIEL"
BASE COUNT          507 a         1065 c          978 g          487 t
ORIGIN      
        1 cccggcgcga cgcgcgcgat gaagctgccg tccgccgcgc tcctcgactc ggagacgatc
       61 tgcccggcgc cgtcccgcaa gaccttcacc agcgcgcccg cgagcggctg gccgcgcccg
      121 tcgagcacgg tgcccgtgat gacgccgtag gggcggcggg tgctgccgcg gttggcggcg
      181 gctgaggcgt cgagcggccc ggccccggcc acgacgatag ccgcgacgta gagcagaccg
      241 acgcgccaga aagatttatt catggaagtt gaaacctccg ggtgtgacac cgggctgaaa
      301 ggcccctccg cgctcagctt tcctgtgttt cggacgcccc ggactttcct tcccacttct
      361 tcgctcgaag gtcggggcgg cgcgtgcggg gcgagggggc ggggccgctt ttccgtggaa
      421 cccttcatgc cagcggcctc cgtcagcccg acggtcatca acgagtgaca cacacttgtc
      481 ggcaagggta tgtccaaccc gcttccggcg gagagggcat tttccgccat cggcgcaaac
      541 agtgtcaagc acttgcacac ctggttagcc gctatgatgc ggcgacggcg cgcattggtt
      601 tgccccctca ttttcggcgt gttacgatgc cgcgtgccgc aaaatacagc aattcctcgg
      661 ttcaccggcc ataattttcc cgaacgaagc tctacagaaa taatgaccga actggttcga
      721 gcgacgagat taccgacggg ccttaccgtc ctgacagagt tcatgccgtc cctgcgctcg
      781 gtgacggtcg gcgtctgggt caggcgcggc tcgcgccacg agcgccccga gatgaacggc
      841 atctgccact tcatcgagca cgcgctcttc aagggcacgc ggcggcgcac ggccctcgac
      901 atcgccgtcg aatccgaccg cctcggcggc cacttcgacg cctacacgac gcacgagctg
      961 accggcttcg ccatgaaggt cgtggacacg gccctgccgc aggcgttcga cctcctctcc
     1021 gacatgctcg cgcggccccg cttcgacgag gaagacctgg agcgcgagcg gcgcgtcatc
     1081 atcgaagaga tgaagatggt cgaggacacg cccgacgaat acctgatcga gctgttctcc
     1141 gccgaatact tccccggcca ctcgctcggg cggcccatcg aagggacgga agagaccgtc
     1201 tcctccttcg accgcgagcg caccgcccgc tatcacgcct cggcctacgc gccgcgcaac
     1261 ctcgtcgtcg ccgccgccgg caacgtcgag cacgacgaac ttgtcgaact cgccgcgcgg
     1321 gtgtttgatg agaggctcgc tgaaggggcg cgggcagagt cggaggcgga cgagggcgag
     1381 ccggaagtgc cgcccgtaca cgcggcgccg attctgattc gacgcaagaa ggaactcgaa
     1441 caggcgcacc tgatactcgc gtcgccgtgg ccctcgtcgc gcgacgagga tcgctacgcg
     1501 gccagcctgc tcggctcggt catcggcggc ggcacctctt cgcgcctctg gcagcgggtg
     1561 cgcgaggagc gcggcctcgc ctactcggtc ggcgcggcgg cgagccagtt caccgacgcg
     1621 ggcgtcttcc agatttacgc gggcacctcg cccgaacacc tcgacgaagt gctcgacctc
     1681 tcgatggagg agctgcggcg cgtcgcgcgc gagcgcgtcg gcgaggacga gctgcggctc
     1741 gtcaaggatc aggcggtctc gtccatcctg ctctctctgg aatcgacgag cgcgcgcgcg
     1801 ggcgcactcg cccgtcagga gatcgtccac ggtcgccgca tcgcgcccga cgaggtcatc
     1861 gcgcgctacg aggccgtcac gcccgacgac ctgctgcgcc tcgcgcggca gttcttccgc
     1921 ggggacgcgc tcgccctcgg cgccctcggc gacctcaacg gcttccgtgt cgagcgctcg
     1981 cggctggaaa tctgaaaggc cgtaagccgt gaaccgtaaa ccgtgacagg ttagaatccg
     2041 gttcttcact cgtcacgctt cgcggttcac gcttcactgc ttttcacttg tcacggttca
     2101 cggttttcgg tttacggctt tcatgaggct caccgtctta ggcagcggct cgacgggcaa
     2161 cgccgtgctg atttgcgcgg gcgagacgcg cgtgctggtg gacgcgggga tgagcgcgag
     2221 ggagacggcg cggcggctcg cgctcgtcgg cgaggacgcg gcgcggctcg acggcgtcat
     2281 catcacgcac gagcacggcg accacgcggg cggcctgcgc gtcctgctga agagtctgga
     2341 gtgtcccgtc tacatctcgg gcgcgacgcg cgacgcctac atccgcgaga agtcgcgcca
     2401 caacgacgag ccgcgccgcc gcgccgaagt cctgcgcgac cgcaccgagg agatttcgtc
     2461 gagcgaggac ttccgcatcg gcgagatcga cttccacccc ttcaccatcc cgcacgacgc
     2521 ggcggacaac ttcggcttcg tcgccactca cgcgggcgtg cgcgtcgcca cgctgatgga
     2581 cttcggccac gtcacgacgc tcctcgccga acgcctgcgc ggctgcgcgg ccatcgtcat
     2641 cgagtcgaac cacagccgcg acatgctcaa ggcgtgcgac gtgtacccct gggagctgaa
     2701 gcagcggata ctttcgcgca ccggccacct ctcgaacgag gacgtggccg cgtggctccg
     2761 cgacggcttc gacggcgcgg cagaccacgt cgtcctcgcg cacctctcgc agcgcgccaa
     2821 caacccgcac gtcgccaagc tcaccgccga gatggcgctc cgcgagcgcg gcggactctt
     2881 ccggacgacg accgaagtca cgctctcgca ccacaaagag ccgacgtcgt ggatagagct
     2941 atgaagaagg cacgaggcaa gaggcacaag gcacaaggca aaaccgaaag gcgcaacccg
     3001 ctctttattt ttgccttttg ccttgtgcct cttgcct
//
LOCUS       DBNGOH010000569         5327 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1734186, whole genome shotgun sequence.
ACCESSION   DBNGOH010000569 DBNGOH010000000
VERSION     DBNGOH010000569.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5327)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5327)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5327
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1734186"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1777
                     /locus_tag="AC159P_11015"
     CDS             <1..1777
                     /locus_tag="AC159P_11015"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TPR end-of-group domain-containing protein"
                     /protein_id="HIR3696021.1"
                     /translation="CVHTLRKALGEGRGEHPYIETVPKSGYRFVAEVKAERDAGGVPD
                     ARRDNGAGNIAGEKRDLMAPEAEPSVAHPEDEARVRDIAPPRAAPRRRPATATVLNSL
                     SALVLTTAAVAFFLITRDRQVVTDETPIDSVAVLPFVNVSGDPGAEYLSDGISDSVIS
                     SLSRIRKLRVISLNSVLRYKGKQVDPQAVGRELNVRAVLAGRLVQRGDVLTISTELVD
                     VRDGRRLWGGQYIRGPSDILAVQGEIAREISSGLRLRLSGEENKKLAKQYTEKAEAYQ
                     LYLLGRHHFRKQTKEGFEQSIDYYERAIKADPNYALAYAGLARAYHWMGTRGFWPAKE
                     VEQKTEWAALKALQLDDTLAEGHVRLGVLKLNHFDWAGAEKELTRAMELDPNSAEANS
                     AYFQFLAGLGRTDEALRYVIRAQELDPTVTPGNLALAYLLARQYDKAIESYRQEIEKR
                     PDDAHMHVLLGEAYVAKGMAEEGVAAVQKGVGLDPSLAKTPERWDRYPMLAYAYAVAG
                     RRAEALKILDEQKRLTRQRHVSPYNFAIIYTGLGDKDQAFDWLGRSIEQRTPLVYRLK
                     VRPMFDPLRSDPRYAELLRRMNLEP"
     gene            1879..4965
                     /locus_tag="AC159P_11020"
     CDS             1879..4965
                     /locus_tag="AC159P_11020"
                     /inference="COORDINATES: protein motif:HMM:NF051698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR3696022.1"
                     /translation="MLLAVVPAHAQAPTGTLTGVVVDSAGTPVAGARLRLTHRDTGLT
                     RNLTSSAEGDYSAAALPPGTYHIMAEADGFRTLERTTAVETGMTTTLNHTLQVGELSE
                     TVTVNAAAPLMQYEHHQVAGLVGRGQIDGLPLNGRNFLELAKLEPGVQPPTRAASNRT
                     FVPALGQPGGQNSGRGTRVTVDGGSIMAVGNGGSAMGFSQEVVHEFQVTTVNFDLTTG
                     LTNGAAINVTTRSGGNELHGTAFYFFRDHTLSAYPALRRDPADPDPFFQRRQFGFALG
                     GPLRRDRFFFFGTYERNEQRGVVATTLQTPEFAHLSRITPSPYFGTQLSLRLDGRLSD
                     AHTAFARYSHDGVRAFGPPTTQPNAYPSAWSRQPAWADQSILGLTSVFGDRVVNDLRF
                     SFFYVSSSQAAPTEQECPGCLGLGAPSITVTQTGLSLGRSLIQQTLGRRYHLNDYVTW
                     QRAAHRTRFGADWEFNRGGTLAVRDEPATLTLFSPQRARQAGIPLPTAFRTLDDILQL
                     PLRSVTVGVGEPRVPQENGSLVRSWHTLRLFLQDTWRLHPRLTVNYGLGWNIDRYQNY
                     DLSKPALLAPLLGADGLGPTRRQWKNFSPVLGAAWSPGRDEKTVVRGGAGFYYDFFFG
                     LTLDNERALLGPPGVGRQNISGDRLLNCLPGIPGVPGGVPLNFPNTPTRFTGAHLLAC
                     LPAIRAALARDLANADQSIQAIQLTKQGAFNPVDVPSMSALHVNLGIQREVARDFVVS
                     ADFAYRQFDHVGLDQLDLNRFDSARGPVIPKCLNEAQRNDPHALCSTGPIIVQQAVGR
                     ATYKGLLLRADKRFPSGLQLLSSWAYSSNTGTNTGNGFNLDDRLGSRGPLANDVTHIL
                     NLAGVVELPWSFRLGFNFSYASAPPFSAFVGGSDFNGDGTTDDLLPGTTVNAFNRRLG
                     REELGRLVVQFNQTNAGKVDAKGAPIPRLVLPQRYWFGDNFHSLDLRLSRAFDIEKRW
                     RVTLIGEVFNVYNAANLSGHSGNLNSVGFGQPGARFTQVFGSGGPRAFQLGARFSF"
BASE COUNT          944 a         1760 c         1684 g          939 t
ORIGIN      
        1 ctgcgtccac acgctgcgca aggcgctggg agaagggcgc ggcgagcacc cgtacatcga
       61 gacggtgccg aaaagcggct accgctttgt ggccgaggtc aaggcggaaa gggatgctgg
      121 cggggtgccc gacgcgcggc gagacaacgg cgcgggtaac attgccggag agaagcgcga
      181 cctaatggcg cctgaggccg aaccgtcagt ggcccatcca gaggacgagg cgagagtgcg
      241 cgacattgca ccgccgaggg cggccccccg gcgtcggcct gccaccgcga ccgtcctcaa
      301 ctctctgtcg gcgctcgttc tgacgaccgc cgccgtcgcc ttcttcctga tcacgcggga
      361 tcgccaagtc gtcaccgacg agactccgat tgactccgtc gccgttctgc ccttcgtgaa
      421 cgtgagcggc gaccccggcg ccgagtacct ctcggacggt attagcgaca gcgtcatcag
      481 cagcctctcg cgcatccgga aactgcgggt catctcgctc aactcggtcc tgcgttacaa
      541 gggcaagcag gttgacccgc aggcggtcgg gcgggaactg aacgtgcggg cggtgctggc
      601 gggcagattg gtgcaacggg gggacgtgct gactatcagc accgagctag tggacgtgag
      661 ggacggccgc cggctgtggg gcgggcaata catccgcggg ccgtcggaca tcctcgccgt
      721 acagggcgag atcgcgcggg agatttcatc agggctgcgg ctgcgcctgt cgggcgaaga
      781 gaacaagaaa ttggccaaac agtacacgga gaaggccgag gcttaccagc tttacctctt
      841 gggcaggcat cacttccgta agcaaacgaa agaggggttt gagcaaagca tcgactatta
      901 cgagcgggcg atcaaagcag acccgaacta cgcgctggct tatgccgggt tggcccgcgc
      961 ctaccactgg atgggcacgc gcggcttctg gcccgcgaag gaggtcgagc aaaagaccga
     1021 atgggcggcg ctcaaggcgc tccagcttga cgacacgctg gccgaaggtc atgtccgtct
     1081 gggggtgctt aagttaaatc atttcgattg ggcgggggcg gagaaagagc tcacacgggc
     1141 catggaactc gacccgaact ccgctgaggc taactcggcg tatttccagt tcctggcggg
     1201 tctcgggcga acggacgagg cccttcggta tgtgatacgc gctcaggaac tcgacccgac
     1261 ggttacaccc gggaacctcg ccctcgctta cctccttgcg cgccagtatg acaaggcgat
     1321 tgagtcgtac cggcaggaga tagagaagag gccggatgac gcccacatgc acgtcctact
     1381 cggggaagcg tatgttgcga aggggatggc tgaggagggc gtcgccgcgg tgcaaaaggg
     1441 tgtggggctc gacccgtctc tcgctaagac cccggagcgg tgggacagat acccgatgct
     1501 ggcctacgcc tacgccgtgg cgggccggcg cgccgaggcg ctgaagatac tcgacgagca
     1561 gaagcggctg acgcggcaac gccacgtctc gccctacaac ttcgccatca tctacacggg
     1621 cctcggcgac aaggatcagg ccttcgattg gctgggaaga agtatcgagc agcggacgcc
     1681 gctcgtctac cgcctcaagg tccgaccgat gttcgacccc ctgcgctcgg acccgcgtta
     1741 cgccgaactg ctccggcgca tgaacctcga accataagcg ggttggctga aaatgccgat
     1801 tcgccggggc cttcatttgc ggctctgccg cgactactca cgacgttgaa taggattcga
     1861 gtacagcttc tcctcgtgat gctcttggcg gtcgtcccgg cccacgccca ggcgccgacc
     1921 ggcaccctaa ccggcgtcgt tgtggactct gccggcacgc ccgtcgccgg ggcgcgcctg
     1981 cgtctcaccc accgcgacac cggactgacg cggaatctca cctcctctgc cgaaggtgat
     2041 tacagcgcgg cggcgttgcc cccgggtacc tatcatataa tggccgaggc cgatgggttc
     2101 cgcacgctcg aacgcaccac cgcagtcgag acgggcatga ccaccacact caaccacacg
     2161 ctccaggtcg gcgagctgag cgagacggtg acggtgaatg cggccgcccc gctgatgcaa
     2221 tacgagcacc atcaggtggc agggctagtc ggccgcgggc agatcgatgg cctgccgctc
     2281 aacggccgca acttcctcga actggccaaa ttggagccgg gcgtccagcc cccgacgcgc
     2341 gccgccagca accgcacctt cgtgccggct ctgggccagc ccgggggcca aaacagcggg
     2401 cgcggcacgc gcgtcaccgt ggacggtggc agcatcatgg ccgttggcaa cggcggctcg
     2461 gcgatgggct tctcgcaaga ggtagtccat gagtttcagg tcaccactgt caacttcgac
     2521 ctcacgacgg gccttaccaa cggcgcggcc atcaacgtga cgacgcgctc gggcggcaac
     2581 gagctgcacg gcactgcctt ttacttcttc cgtgaccaca cgctgtccgc ctaccccgcg
     2641 ctcagacgcg atcccgccga cccggacccg ttcttccagc ggcggcagtt cggctttgcc
     2701 ctcggcggcc ccctgcggcg cgaccgtttc ttcttcttcg gcacctacga gcgcaacgag
     2761 cagcggggcg tggtggcgac gacgctgcag acgccggagt tcgcgcacct gagccgcatc
     2821 actccgagcc cgtacttcgg cacgcagctc agcctgcgcc tcgacggccg cctctccgac
     2881 gcccacacgg ccttcgcccg ctactcgcac gacggagtcc gcgccttcgg gccgccgacg
     2941 actcagccga atgcttaccc gtcggcctgg tcgcgccagc cggcctgggc cgaccagagc
     3001 atcctgggcc tcacgagcgt cttcggcgac agggtggtta acgacttacg cttttcgttc
     3061 ttttacgtca gctccagcca agccgcgccg accgagcagg aatgtcccgg ctgcttgggc
     3121 ctcggtgcgc cttcaattac cgtgacacag accggcctgt ctctcggtag atccttgatt
     3181 caacagacgc tcggccggcg ttaccacctc aacgactacg tgacctggca gcgggctgcg
     3241 caccgcacgc ggttcggcgc tgactgggag ttcaaccgcg gcgggactct cgcggtcaga
     3301 gacgagccgg cgaccctaac gctcttttcg ccgcagcggg cacgccaagc gggcatcccg
     3361 ttgccgacag ccttccgcac gttggacgac atccttcagc tcccgttgcg aagcgtcacc
     3421 gtcggcgtcg gtgagccgcg cgtgccacag gagaatggga gcctggtacg gagctggcac
     3481 accctccggc tcttcctcca ggacacctgg cgactccatc cacgactgac cgtcaactac
     3541 gggctgggct ggaacattga ccgttatcaa aactacgacc tctcgaaacc cgcgttgctg
     3601 gcgccgctcc tgggcgcgga cggcttaggc ccgacgcgca ggcagtggaa gaacttctcc
     3661 ccggtgctgg gggcggcttg gtcgccgggg cgcgacgaaa agaccgtggt tcgcgggggt
     3721 gccggcttct actacgattt cttcttcggc cttacccttg ataacgagcg cgcgctgctc
     3781 ggcccacccg gggtggggcg tcagaatatc tccggcgaca ggctcctcaa ctgcctgccg
     3841 ggcatccccg gcgtccccgg cggggtgcct ctgaacttcc ccaacacgcc gacgcggttc
     3901 accggcgcgc acctgctggc ttgtctgccg gcgatccgtg ctgcgttggc acgggacctg
     3961 gccaacgcgg atcaatccat tcaggcgatc cagttgacca agcagggcgc cttcaatccc
     4021 gttgacgttc cgagcatgtc tgcgctccac gtcaacttag ggattcaacg ggaggtcgcg
     4081 cgggacttcg tcgtgagcgc cgacttcgcc taccggcaat tcgatcacgt tgggctcgac
     4141 cagctcgacc ttaatcgttt cgacagcgcg cgcggccccg taattccgaa gtgtctcaac
     4201 gaggcccagc gcaacgaccc gcacgcactc tgctcgacgg ggccgatcat agtgcaacaa
     4261 gccgtgggcc gagcgaccta caaggggctg ctcctcaggg ctgacaagcg cttcccgagc
     4321 ggccttcagc ttttaagctc gtgggcgtat tcgagcaaca ccggcaccaa cacggggaac
     4381 ggcttcaacc tcgacgaccg gctgggcagc cgtggcccgc tcgccaacga cgtcacgcac
     4441 atcctgaatc tggccggcgt cgtcgagttg ccgtggagct tccggctcgg gttcaacttc
     4501 tcttacgctt ccgccccgcc gtttagcgcc tttgtcggcg ggagcgactt caacggcgac
     4561 ggcacgacgg acgacctgct gcccggcacc accgtcaacg ccttcaaccg ccgcctgggg
     4621 cgtgaggagt tggggcgcct cgtcgtgcag ttcaaccaga ccaacgccgg caaggttgac
     4681 gccaaaggcg caccgatccc tcggttagtg ctgccgcagc gttactggtt cggcgacaat
     4741 tttcactcgc ttgatctgcg gctgagtcgg gcgttcgaca ttgagaagag gtggcgtgtg
     4801 acgctcatcg gcgaggtctt caacgtctat aatgccgcaa acctttccgg ccacagcggc
     4861 aacctcaaca gcgtcgggtt cggccaaccc ggcgcgcgct tcacgcaggt cttcggctcc
     4921 ggagggccgc gcgcttttca gctaggggcg cgctttagct tttgaggagt tttggaatga
     4981 cagggattct gccggggggc gtttcggtaa tcttcctctc gaacattgat ccgaccgccc
     5041 gccggattct caacacgagt ggttgcaggc gtgtcatctc gacgcgtaac aatccgcgga
     5101 acattcaata agcgtcgaac ttgattttct gacgcagcgg tacgttaact gccccgttta
     5161 ttcattcagt tatctcaatc ggcgggcttc ggatagctga attgttacct tgtgtcaagc
     5221 ggaggccaaa acatccgcgg cttgttttgc cgtcagcacg cggttgtaga tgggcgttcc
     5281 cggctgttgt agtctgccgc cttcgcggag ggggccggtg tcgaagg
//
LOCUS       DBNGOH010000570         2386 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4482714, whole genome shotgun sequence.
ACCESSION   DBNGOH010000570 DBNGOH010000000
VERSION     DBNGOH010000570.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2386)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2386)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2386
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4482714"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1195)
                     /locus_tag="AC159P_11025"
     CDS             complement(<1..1195)
                     /locus_tag="AC159P_11025"
                     /inference="COORDINATES: protein motif:HMM:NF013741.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HIR3696023.1"
                     /translation="MSTLYIALVIFLVLANGFFVASEFALVGVRRSRIETLAAAGNRS
                     ARRTLLLLDNLNAYISATQLGITMASLALGWIGEPVVAHMLEEPLRGRVSETVLHTIS
                     FTIAFTIITFLHIVLGELAPKTMALERAEAVSLAISWPLHTFYKIFQWPIRLLDWAGT
                     RTVRLFGLHPSQDHASVYTEEELRHLVDISRKSGHLEAQEQQLINRVFEFNDAEVREA
                     MVPRTNVYALPLTATLEETKTAFRTLGYSRLPVYRERIDEAVGVVFRRDIEPYLDGTR
                     ADEFDIEKLLHPPTFIPAAARLGATLQQMQQTRTHLAFVIDEHGGLEGIVTLEDLLEE
                     IVGEINDEYDEEAKSQIVEQPDGTYLLDGMLAVRDANRQFELGLPEDGNYTTLAGFLQ
                     ARTGRL"
     gene            complement(1368..>2386)
                     /locus_tag="AC159P_11030"
     CDS             complement(1368..>2386)
                     /locus_tag="AC159P_11030"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HIR3696024.1"
                     /translation="QLKTPPPIRQDEDPDPQGEREVGEGEVIKTDVNLVNLQVRVVDR
                     HNRPIGDLRADEFRVFENGVQQKIEFVTREEVPISYGIVVDNSGSLRNQINQVIEASK
                     TIVSSNKPGDETFVVRFISSDEIKIMQDFTADQQSVTDALDDMFVEGGQTAIIDAVYL
                     SSEHAARHKRGNPLEDKRRRALVLVTDGEDRNSFYKRDQLFESLRENDVQIYVIGFVN
                     ELDREGGFISKSKREKATDLLNRMAKETGGRAFYPNSLTELPGIAEEITKDLRTQYVL
                     SYAPAVPARAGEYRQVRVAVADAPGRDKRIAITRSGYTARGGGGGAAPPAQTGNRPAS
                     AKKP"
BASE COUNT          378 a          725 c          852 g          431 t
ORIGIN      
        1 ggaggcgccc ggtgcgcgcc tgcaggaagc cggcgagcgt cgtgtagttg ccgtcttcgg
       61 ggaggcccag ctcgaactgg cggttggcgt cgcgcacggc gagcatcccg tcgagcaggt
      121 acgtcccgtc cggctgctcg acgatctgcg acttcgcctc ctcgtcgtac tcgtcgttga
      181 tctcgccgac gatctcttcg agcaaatcct ccagcgtgac gatgccttcg aggccgccgt
      241 gctcgtcgat gacgaaggcg aggtgcgtgc gcgtctgctg catctgctgg agcgtggcgc
      301 cgagccgggc cgccgcgggg atgaaggtcg gcgggtgcag cagcttctcg atgtcgaact
      361 cgtcggcgcg tgtgccgtcg agatatggct cgatgtcgcg gcggaagacg acgccgacgg
      421 cctcgtcgat gcgctcgcgg tagacgggca ggcgcgagta gccgagcgtg cggaaggcgg
      481 tcttcgtctc ttcgagcgtg gccgtcaagg gcagcgcgta cacgttcgtg cgcggcacca
      541 tcgcctcgcg cacctctgcg tcgttgaact cgaagacgcg gttgatgagc tgctgctcct
      601 gcgcttcgag gtggccgctc ttgcgcgaga tgtccacgag gtggcgcagc tcctcctccg
      661 tgtagaccga ggcgtgatcc tgcgaggggt gcaggccgaa gaggcgcacg gtgcgcgtgc
      721 ccgcccagtc gagcaggcgg atcggccact ggaagatttt gtagaaggtg tgcagcggcc
      781 acgagatcgc cagcgagacg gcctcggcgc gttcgagcgc catcgtcttc ggcgccagct
      841 cgcccagaac gatgtgcagg aacgtgatga tcgtgaacgc gatggtgaaa gagatggtgt
      901 gcagcaccgt ctcggagacg cgtccgcgaa gcggctcctc caacatgtgc gccacgaccg
      961 gctcgccgat ccagcccagc gcgagcgacg ccatcgtgat gccgagctgc gtcgcggaga
     1021 tgtaggcgtt cagattgtcg agcagcaaga gcgtacgccg cgcgctgcgg ttgcccgcgg
     1081 cggccagcgt ctcgatgcgc gagcggcgca cgccgaccag cgcgaactcc gacgcgacga
     1141 agaagccgtt ggcgaggacg agaaaaatca cgagggcgat ataaagcgtt gacatcatgc
     1201 gattattcta ccgggaaggg ggggaaggaa gaaggggtaa aaggggaaaa ggggaaaagg
     1261 cgaaaaggga aagggcgaaa agggaaaggg cgaaaaggga aagacagcaa gccgcttgtc
     1321 tcccttttcg ccctttcccc ttttaccctt ttaccccttt gatcgtgtta cggtttcttg
     1381 gcggaggcgg gacggttgcc ggtctgggcc gggggggcgg cacctccgcc gccgccgcgc
     1441 gccgtgtagc cggagcgcgt gatggcgatg cgcttgtcgc ggcccggcgc gtcggcgacg
     1501 gcgacgcgca cctgccggta ctcgcccgcg cgcgccggca ccgcgggcgc gtaggacagg
     1561 acgtactgcg tgcggaggtc tttggtgatc tcctcggcga tgcccggcag ctccgtgagc
     1621 gagttcgggt agaaggcgcg cccgcccgtc tccttcgcca tccggttcag caggtcggtc
     1681 gccttctcgc gcttcgactt gctgatgaag ccgccctccc ggtcaagctc gttgacgaag
     1741 ccgatgacgt aaatctgcac gtcgttctct ctgagcgact cgaagagctg gtcgcgcttg
     1801 tagaagctgt tgcggtcctc gccgtccgtg acgaggacga gcgcgcgccg ccgcttgtct
     1861 tcgagcgggt tgccgcgctt gtggcgggcg gcgtgctccg acgagaggta gacggcgtca
     1921 atgatagccg tctgcccgcc ctcgacgaac atgtcgtcga gggcgtcggt gaccgactgc
     1981 tggtcggccg tgaagtcctg catgatcttg atctcgtcgc tggagatgaa gcgcacgacg
     2041 aaggtctcgt cgcccggctt gttgctcgag acgatggtct tcgaggcctc gatgacctga
     2101 ttgatctggt tgcggagcga gcccgagttg tccaccacga tgccgtagga gatgggcacc
     2161 tcctcgcgcg tcacgaactc gattttctgc tggacgccgt tctcgaaaac gcggaactcg
     2221 tcggcgcgca ggtcgccgat gggccggttg tggcggtcca cgacgcgcac ctggaggttg
     2281 acgaggttga cgtcggtctt gatgacctcg ccctcgccca cctcgcgctc gccctgcggg
     2341 tcggggtctt cgtcctggcg gatgggcggc ggcgtcttga gctgcg
//
LOCUS       DBNGOH010000571         4577 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4116366, whole genome shotgun sequence.
ACCESSION   DBNGOH010000571 DBNGOH010000000
VERSION     DBNGOH010000571.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4577)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4577)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4577
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4116366"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..460)
                     /locus_tag="AC159P_11035"
     CDS             complement(<1..460)
                     /locus_tag="AC159P_11035"
                     /inference="COORDINATES: protein motif:HMM:NF025292.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin-like domain-containing protein"
                     /protein_id="HIR3696025.1"
                     /translation="MSSKAEAKKPSAAKAKAGAKKPSAKQLQKQLAGTWSLVSAEDFD
                     QQTGKWVPYTFGNPPSGYFIYDATGHASIQIMTTPPVALATPDTPTPQESLAIFNGFI
                     AYYGTYTVDASNISVQVEGAWDPSQVGTPQVRPYQLKGDKLIIGDQKTYRR"
     gene            891..2378
                     /locus_tag="AC159P_11040"
     CDS             891..2378
                     /locus_tag="AC159P_11040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696026.1"
                     /translation="MGKTPSERARAFVLTLAAAAFALMHYGCQPQPATAPNANGATPA
                     ASPAPTAAQVVIPPWPQGFPQGFPAAAISGHLPLAVTLPDGNVTPEQARPYFDYFSWE
                     SFIALNWPASTSQRGTPDQPNNPNAFLKAPAGAPVVWGTYKDSFDLFGQKGDQRPPAW
                     DAPNTSPCPNAQGSEKTLAFITKGDTPLMQTAQAFSFPLVDQRGNYVYYDIRYDQAQY
                     NFIRGQDSDPSSWLYLLKNLAAKENVPKGLQMPMSTTTPSPVLGSIMVKAAWRIKTER
                     DDPTRFYTVNAQVYNPQTKACTEMPVLLVGLHVAHKVSPFTEWVWSTFEQVDNVPPDP
                     DITPRPTPPPAGYSFNNGTDTPKTNGGFDYKPPLQPVPSPSPVQVTRVNPIPSTPQGA
                     STRDVNAYFQQLLKGTVWQYYQLVITQWPSNPGLDAFVLMQNGGVYPRDSGQAFPANG
                     AVNTTQETYFQTPNDAAGAGGNSCMSCHYRAGQSDYSWGLNRRAH"
     gene            2378..4297
                     /locus_tag="AC159P_11045"
     CDS             2378..4297
                     /locus_tag="AC159P_11045"
                     /inference="COORDINATES: protein motif:HMM:NF024753.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB
                     family protein"
                     /protein_id="HIR3696027.1"
                     /translation="MDKKSELALLKRRNTPQPEAANATATATLPPPATAADEAAPPTS
                     AAQARTGWWMYHGDPEHTGYVSDSDLSTANVNQTSFTTLHTLQLGGPVLSVPAIADGF
                     VYVGTANYQQAEGGNGGALHKIDIQSGAVVQTFAWNLGGDAADAHHFAGMGCTPLVLN
                     GFVYFGAFNGKFYCLDQETLQQVWVTDLRNEDLAHNQPVTNINGVSDVGYPPAVIWTS
                     PVVSADGTKLYVGCGEGENPELYSFVFCLDTANGNVNWIYCTNLFCTGESNQPNVLPE
                     QAVQVLPPPAGFSVFKGEPIVMGCSVWGAIAYDKDLHRVYCPTGNQQPEPSGYWQEFF
                     GPFKPELPSPGFSNGLLSLDAETGEYKAFFQVTPESNYRPSDFDIDIGSAPVIFDLEG
                     RNCVVLTCKNGGLFILDADTLELIRWRQLLPYMNDGTWVESVDKHPKDQNALNPHVTN
                     DESNATPGENFSGAFNTAAHHPGDPSQGISPRLFIGIGGPNYHNASPGIDYETTPFVR
                     AVDLSSDTLADAWPLDDSDPRRYVNGSHTDDLTGESGLYTSAGESGISSPAVVNDVVF
                     CTTSKIGIYAFDVRDGKLLWWDDLGMQTDGYNGGYGYCLGAAVWKNYVVAGALVFGRD
                     GGVLRIYGLDPQPTS"
     gene            4325..4549
                     /locus_tag="AC159P_11050"
     CDS             4325..4549
                     /locus_tag="AC159P_11050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696028.1"
                     /translation="MAVSFSNDIMPLFNQFKGQMMWRFDLTDYEHVKANASTIYGRIN
                     GGGMPPPPFDPLTLEQIQLFQQWMEEGYAP"
BASE COUNT          829 a         1649 c         1371 g          728 t
ORIGIN      
        1 tgcggcggta ggttttctgg tcgccgatga tcaacttgtc gcccttcaac tggtagggcc
       61 gcacctgcgg cgtgccgacc tgcgacgggt cccacgcgcc ttcgacctgc acgctgatgt
      121 tggaggcatc gacggtgtaa gtgccgtagt aggcgatgaa gccgttgaag atggcgagcg
      181 actcttgcgg cgtcggggtg tcgggcgtgg cgagtgcgac gggcggcgtc gtcatgattt
      241 gaatggacgc gtggcccgtc gcgtcgtaga tgaagtagcc ggacggcgga ttgccgaacg
      301 tgtacggcac ccacttgccc gtctgctggt cgaagtcctc ggcggagacg agactccacg
      361 tgccggccag ttgtttttgc agttgcttcg cggatggctt tttcgccccg gccttcgcct
      421 tcgccgcgga aggcttcttc gcttcggctt tcgaactcat gctgtgctcc tcgtggggtg
      481 tgagcgaacg cgcgcggcgc cacgcggcga cggcgcgccg aagacaatac acaagaacgg
      541 cgggcgcgac aacgtcgatg cgccgcgcgg cgacggtcga tgcgccgcgc ggcgacgttg
      601 tcggggagcc gccacgtgtt tgacgagcgc ggcgccggcg ggccgcccgt cactcgacac
      661 attgttgttg acgcggcggc gcggcgggtc taacatgcgc ggcgtccaag cagtctcacg
      721 ttagcactta gcatccctcg ttcacgcttg agcgatctcc atcgagagtc ggtaagcctc
      781 ttcggccccg cgccggctgg ccgccggcac gcttgtcgca ggcacacgat gttcacgcga
      841 gcctctgccg aacacttcgg cctcacgtct cgtggataaa ggagcgcatc atgggcaaga
      901 ctccgtccga acgtgccaga gctttcgtcc tgacacttgc cgccgccgcc ttcgctctca
      961 tgcactacgg ttgtcaaccg cagcccgcga cggcccccaa cgcgaacggc gcgacgcccg
     1021 cggcctcgcc cgccccgacc gccgcgcagg tggtcatccc gccgtggccg caaggcttcc
     1081 cgcagggctt ccccgcggcg gccatcagcg gccatctccc gctcgccgtc acgctgcccg
     1141 acggcaacgt gacgcccgag caggcgcggc cctacttcga ctacttctct tgggaatcct
     1201 tcatcgccct gaactggccc gcgtcaacga gtcagcgcgg cacgcccgac cagccgaaca
     1261 acccgaacgc gttcctcaaa gcgcccgccg gggcgccggt cgtgtggggg acttataaag
     1321 actcgttcga cctgttcggg cagaagggcg accagaggcc gcccgcctgg gacgcgccga
     1381 acaccagccc gtgcccgaac gcgcaaggca gtgagaagac gctcgccttc atcaccaaag
     1441 gcgacacgcc gctgatgcag accgcgcagg cgttctcctt cccgctcgtc gatcaacgcg
     1501 gcaactacgt ctactacgac atccgctacg atcaggcgca gtacaacttc atccgcgggc
     1561 aggactccga cccctcttcg tggctctacc tcttgaagaa cctcgcggcg aaggagaacg
     1621 tgccgaaggg cttgcagatg ccgatgagta cgacgacgcc ctcgcccgtg ctcggctcga
     1681 tcatggtcaa ggccgcgtgg cgcatcaaga ccgaaaggga cgacccgacc cgcttctaca
     1741 ccgtcaacgc gcaggtctac aacccgcaga cgaaggcttg caccgagatg cccgtgctgc
     1801 tcgtcggtct ccacgtcgcg cacaaggtct cgcccttcac cgagtgggtc tggtcaacct
     1861 tcgagcaggt ggacaacgtg ccgcccgacc cggacatcac gccgcggcca acgccgccgc
     1921 ccgcgggcta ctccttcaac aacggcacgg acacgccgaa gaccaacggc ggcttcgact
     1981 ataagccgcc gctccagccc gtgccgagtc cttcgcccgt gcaggtgacg cgcgtcaacc
     2041 cgatcccttc gacgccgcag ggcgcctcca cgcgcgacgt gaacgcctac ttccaacaac
     2101 tgctgaaggg gacggtctgg cagtattacc aactggtcat cacgcagtgg ccttccaacc
     2161 ccggcctcga cgccttcgtc ctgatgcaga acggcggcgt ctacccgcgc gactcgggcc
     2221 aggccttccc cgcgaacggc gcggtcaaca cgacgcagga gacctacttc cagaccccga
     2281 acgacgccgc cggggcgggc ggcaacagtt gcatgagctg tcactacagg gccggccaat
     2341 ctgattacag ttggggcctg aaccggaggg cacactgatg gataagaagt ccgaactcgc
     2401 gctgctcaaa cgcaggaaca cgccgcaacc cgaagccgcc aacgcgaccg ccaccgccac
     2461 gctgccgccg cccgcgaccg ccgccgacga ggccgcgccg ccgacctccg ccgcgcaggc
     2521 gcgcaccggc tggtggatgt accacggcga cccggagcac acgggctacg tctccgacag
     2581 cgacctctcg accgcgaacg tcaaccagac ttcgttcacg acgctccaca cgctccagct
     2641 cggcggcccc gtcctctccg tgcccgcaat cgccgacggc ttcgtctacg tcggcaccgc
     2701 caactaccag caggcggagg gcggcaacgg cggcgcgctg cacaagatcg acatccagag
     2761 cggcgccgtc gtccagacct tcgcgtggaa cctcgggggc gacgccgcgg acgcgcacca
     2821 cttcgcgggc atgggctgca cgccgttggt cttgaacggc ttcgtctact tcggcgcctt
     2881 caacggcaag ttctactgcc tcgatcagga gacattgcag caggtctggg tcaccgacct
     2941 gcgcaacgaa gacctcgcgc acaaccagcc ggtgacgaac atcaacggcg tctccgacgt
     3001 gggctacccg cccgccgtca tctggacttc gccggtcgtc tcggcggacg gcaccaagct
     3061 ctacgtcggc tgcggcgagg gcgagaaccc cgagctgtac agcttcgtct tctgcctcga
     3121 caccgcgaac ggcaacgtca actggattta ctgcacgaac cttttctgca cgggcgagtc
     3181 caaccagccg aacgtgctgc ccgagcaggc cgtgcaagtg ctgccgccgc ccgccggctt
     3241 cagcgtcttc aaaggcgagc cgatcgtgat gggctgctcg gtctggggcg cgattgctta
     3301 cgataaggat ttgcaccgcg tctactgccc gaccggcaac cagcagcccg agccgagcgg
     3361 ctactggcag gaattcttcg gccccttcaa gcccgagctg cccagccccg gattctccaa
     3421 cggcctgctc tcgctcgacg ccgagaccgg agaatacaaa gccttcttcc aggtcacgcc
     3481 cgagagcaac taccgcccct ccgacttcga catcgacatc ggcagcgcgc ccgtcatctt
     3541 cgacctcgaa gggcgcaact gcgtcgtcct cacgtgcaag aacggcggcc tcttcatcct
     3601 cgacgccgac acgctcgaac tcatccgctg gcgccagctt cttccgtaca tgaacgacgg
     3661 cacgtgggtc gagtccgtag acaagcaccc caaagaccag aacgccttga acccgcacgt
     3721 caccaacgac gagtcgaacg ccacccccgg cgagaacttt tcgggcgcct tcaacaccgc
     3781 cgcacaccac cccggcgacc cttcgcaggg catctcgccg cgcctcttca tcggcatcgg
     3841 cgggccgaac taccacaacg cctcgcccgg catcgactac gaaacgacgc ccttcgtccg
     3901 cgccgtcgat ctctcgtccg acacgctcgc cgacgcgtgg ccgctcgacg actcggaccc
     3961 gcgccgctac gtcaacggct cgcacacgga cgacttgacc ggcgagagcg ggctgtacac
     4021 ctccgcgggc gagagcggca tcagctcgcc ggcggtcgtc aacgatgtgg tcttctgcac
     4081 gacgagcaag atcggcatct acgccttcga cgtgcgcgac gggaaacttc tgtggtggga
     4141 cgacctcggc atgcagaccg acggctataa cggcggctac ggctactgcc tcggcgccgc
     4201 cgtctggaag aactacgtcg tggcgggcgc gctcgtcttc gggcgcgacg gcggcgtgct
     4261 gcgcatctac ggcctcgacc cgcagccgac ttcatagaca gattgtgtga gggaggactt
     4321 ctcgatggct gtctctttca gcaacgacat catgcccctc ttcaaccagt tcaaggggca
     4381 gatgatgtgg cgcttcgacc tgaccgacta cgagcacgtc aaggccaacg cctcgaccat
     4441 ctacggtcgc atcaacggcg gcggcatgcc cccgcccccc ttcgacccgc tcacgctcga
     4501 acagattcaa ctcttccagc agtggatgga agaaggctac gccccctgag agaaggcgaa
     4561 aaggttaaaa ggggaaa
//
LOCUS       DBNGOH010000572         4638 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_451486, whole genome shotgun sequence.
ACCESSION   DBNGOH010000572 DBNGOH010000000
VERSION     DBNGOH010000572.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4638)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4638)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4638
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_451486"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..112)
                     /locus_tag="AC159P_11055"
     CDS             complement(<1..112)
                     /locus_tag="AC159P_11055"
                     /inference="COORDINATES: protein motif:HMM:TIGR01409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HIR3696029.1"
                     /translation="MTASRRDFLKTAGLTVGAAALLPQSVFEAEAAVDARL"
     gene            complement(343..1440)
                     /locus_tag="AC159P_11060"
     CDS             complement(343..1440)
                     /locus_tag="AC159P_11060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696030.1"
                     /translation="MRHRLNKMLLVAALAVLALCGGGALTGSPSSAQSSTPGTWTIYP
                     AQTTTYTTSVRPPIKANGSSVFNGSSVIPVKFKLSSAPGATKFESVFSNNENGPNGEE
                     PIPDDDFSFLSFEPDGELLFNQLTTLKATYSFIEGNCGGGSLRWQVRVDILGNNGDPT
                     PTPCDPDDPTCVPPPTPADDDRSVFIYYGDHPNFTNCTTGANDQSGVNLLTLTDLRFD
                     TSQVGGTFYDTLANANSLVGTKQVASASLVLDSGWRQDSLGNYLDQRVNLGGATVNDN
                     QFTPLSGDPTTTCSLPPATISIQKISDGGTPADPISVQRPNSDTNFTINNDCTYSYNL
                     DTASLLGPGTYQVNAVINGSTVATGATFTVR"
     gene            complement(1897..2412)
                     /locus_tag="AC159P_11065"
     CDS             complement(1897..2412)
                     /locus_tag="AC159P_11065"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3696031.1"
                     /translation="MGVEIRLADRLRDEERRQLFEWGEDLFGVGALKVRWRPKELHFV
                     VESAGRAVSHVGILRHTVAVGGRPVTVGGVGGVITVGEAQGSGYASLALERATAFMRD
                     ELDVEFGFLFCRDPLVPFYERHGWHLIAVPVTVEQGEDAPLVMPLNAMVLPFGGRRWP
                     QGPVSLESLPW"
     gene            2555..>4638
                     /locus_tag="AC159P_11070"
     CDS             2555..>4638
                     /locus_tag="AC159P_11070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018127514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40/YVTN/BNR-like repeat-containing protein"
                     /protein_id="HIR3696032.1"
                     /translation="MFGASLFSRASAAAAPLSTKLVAVVSLLLATCFASGVAVLQASA
                     RQQQPTDAPQQGASQPNPLGPLAYRQIGPFRGGRVTAVAGVTAQPFVYYFGATGGGVW
                     KTTDGGVNWEPVGDAHFKTGSVGAIGVAESDPNVVYVGMGESPIRGNVSHGDGMYKST
                     DAGKTWKRLGLEETRHIARVRVHPKNPDLVYVAALGHTFGPNEQRGVFKTTDGGKSWK
                     KSLYKSDKAGAIDLVLDPSNANVLYAGFWEVVRRPWSLESGGPGSGLFKSTDGGDTWT
                     ELTRNPGGPRGLVGKVGVTVSPANPERVWAIIEAEDGGVFRSDNGGRTWTRTNEQRNL
                     RQRAWYYTRIYADTENPEAVYVLNVGFFKSNDGGRTFSQISTPHSDNHDLWIAPNDAN
                     RMIEGNDGGANVSVNGGRSWTEQDQPTAQFYRVAVDDDFPYHIYGAQQDNSTVKIASR
                     TNGFGIGESDWHDVGGGESGWIAPHPKDSDIVFAGSYGGYLTRYDHRTGQQRAVNVWP
                     ENPMGHGAEGMKYRFQWNYPILFSPHDTSNGGVLYAAGDRLFRSTNEGQSWEIMSPDL
                     TRNDKSKQGPSGGPITKDNTSVEYYNTIFTLMESPVTKGVIWAGTDDGLVHVTRDNGK
                     TWSNVTPRGIPEWSQINSVEASPHDAGSAYVAATMYKWDDFRPYLYRTSDYGRTWKKI
                     DNGIPASAFTRVV"
BASE COUNT          778 a         1513 c         1601 g          746 t
ORIGIN      
        1 cgaggcgggc gtcaaccgcc gcctcggcct cgaagaccga ctgcggcagc agcgccgccg
       61 cccccacggt cagcccggcg gtcttcagaa agtcgcgtcg agaagctgtc atgttcttca
      121 ccctctcgcg ggccgtcgag gggccgcgcg ccgtcaggtt agttgaatgt gttttcggag
      181 agctgcgatt cggaatgggg ccactctaat acaggttgac gaaatgtcaa gaggcgagtc
      241 cgccgcgcag tgtccggacg cggggccgtt gaggcgcgaa gggccgcgcc ctcgcgcggg
      301 gcgcggccct ctggcgttcg gtgcggcgcg cgtcggcgcg cgtcagcgca cggtgaaggt
      361 ggcgccggtg gcgacggtgc tgccgttgat gacggcgttc acctgatagg tgccggggcc
      421 gagcagcgac gccgtgtcga ggttgtagga gtaggtgcag tcgttgttga tggtgaagtt
      481 ggtgtcgctg ttcgggcgct ggacgctgat cgggtcagcc ggtgtgccgc cgtcgctgat
      541 cttctggatg ctgatggtcg cgggcggcag gctgcacgtc gtcgtcgggt cgccggagag
      601 cggcgtgaac tggttgtcgt tcaccgtcgc gccgccgagg ttgacccgct ggtcgaggta
      661 gttgcccagc gagtcctgcc gccacccgct gtcgagcacg agcgaggcgc tggccacctg
      721 cttcgtgccg acgagggagt tggcgttggc cagcgtgtcg tagaacgtgc cgccgacctg
      781 cgaggtgtcg aagcggaggt cggtgagcgt aagcaggttg acgccgctct ggtcgttcgc
      841 gccggtcgtg cagttggtga agttcgggtg gtcgccgtag tagatgaaga ccgagcggtc
      901 gtcgtccgcc ggggtgggcg ggggcacgca cgtcgggtcg tcggggtcgc acggcgtcgg
      961 cgtcgggtcg ccgttgttgc cgagtatgtc aacccgcacc tgccagcgaa gcgagccgcc
     1021 gccgcagttg ccctctatga acgagtaggt ggccttgagt gtggtgagct ggttgaagag
     1081 cagttcgccg tccggctcga agctgaggaa cgagaagtcg tcgtcgggga tcggctcctc
     1141 gccattgggg ccgttttcgt tgttcgagaa gacggactcg aacttggtcg cgccgggcgc
     1201 gctcgacagc ttgaacttga cggggatgac cgacgagccg ttgaagacgc tcgacccgtt
     1261 ggccttgatg ggcggtcgca cgctggtcgt gtaggtggtc gtctgcgcgg ggtagatcgt
     1321 ccaggtgccg ggcgtggagg actgcgcgga cgaggggctg ccggtcagtg cgccgccgcc
     1381 gcagagggcg agcacggcca gcgcggccac cagcagcatc ttgttcagcc tgtgtctcat
     1441 cgttacttct ccttcgtttg gggggttggg ggttcgacgc acgacccctg tccgaagacg
     1501 acgggcagga cggtcttcgg acgaagcggg agcgcgacgg ctcggtcgtg cgacctcgac
     1561 tgacgggaag agattcggcc cttctgactg ttgcgcgacg gcggggcgcc ggcaggagtc
     1621 ctgtaagact taaaaacgtg tgcgggctta tatacccaag cccgcccgcg tgtcaacggg
     1681 cggcgggcgg cgggcagtca agacagaaga tgcttgagag gtaaagtccc aatcctcccg
     1741 gctcttccag caatacggcg agaggcttga gattcaacgt ctcaagcctc tcgcttcgac
     1801 tgcttgaagg gcgaaggtgt tcggcacttt acctcccaag tcgcttcgtc cttcttcttg
     1861 actgactgct gcccgctcct cgctgcccgc tgttcttcac cacggcaggc tttcgaggct
     1921 gaccggcccc tgcggccagc ggcgcccgcc gaaggggagc accatcgcgt tgagcggcat
     1981 gacgaggggc gcgtcttcgc cctgctcgac ggtgacgggc acggcgatga ggtgccagcc
     2041 gtggcgctcg tagaagggga cgagcgggtc gcggcagaag aggaagccga actccacgtc
     2101 cagctcgtcg cgcatgaagg ccgtggcgcg ttcgagcgcg aggctggcgt agccgctccc
     2161 ctgcgcctcg ccgaccgtga tgacgccgcc caccccgccc acggtcacgg gccgcccgcc
     2221 gacggcgacc gtgtggcgca ggatgccgac gtggctcacc gcgcggcccg cggactcgac
     2281 gacgaagtgc agctccttcg gtcgccagcg caccttgagc gcgcccacgc cgaagaggtc
     2341 ttcgccccac tcgaacagtt gccgccgctc ctcgtcgcgg agcctgtcgg ccagacggat
     2401 ttcgacgccc atcatttcac cccgcccccg tcaaagagat tgttgccccg cgaagcctgt
     2461 ggcgatatat tgtgcaacac atcgcaagct tttgcattcc gtttcggcgc tcgtcacgca
     2521 agctcgaaca cgaaaaacga aggagcctgt caacatgttc ggggcctctc tcttctcgcg
     2581 cgcatccgcc gccgccgcac cgctctcgac gaaactcgtc gccgtcgtct cgctgctgct
     2641 cgccacgtgt ttcgcgagcg gcgtcgccgt gctccaagcc tcggcgcggc agcagcagcc
     2701 gacggacgcg ccgcagcagg gcgcgtcgca gccgaacccg ctggggccgc tcgcctacag
     2761 gcagatcggc ccgttccgcg gcgggcgggt gacggcggtg gcgggcgtca ccgcgcagcc
     2821 cttcgtctac tacttcggcg cgacgggcgg cggcgtgtgg aagacgaccg acggcggcgt
     2881 gaactgggag cctgtcgggg acgcgcactt caagaccggc tcggtcggcg ccatcggcgt
     2941 cgccgagtcg gacccgaacg tggtctacgt cgggatgggc gagtcgccca tccgcggcaa
     3001 cgtctcgcac ggcgacggga tgtacaaatc gaccgacgcc ggcaagacgt ggaaaagact
     3061 cggcctcgaa gagacgcgcc acatcgcgcg cgtgcgcgtg catcccaaaa acccggacct
     3121 cgtctacgtt gccgcgctcg gccacacctt cgggccgaac gagcagcgcg gcgtcttcaa
     3181 gaccaccgac ggcggcaaga gctggaaaaa atccctctac aagagcgaca aggcgggcgc
     3241 catcgacctc gtcctcgacc cctcgaacgc gaacgttttg tacgccggct tctgggaggt
     3301 ggttcgcagg ccgtggtctc tcgaaagcgg agggccgggc agcggcctct tcaaatcgac
     3361 ggacggcggc gacacgtgga cggagctgac gcgcaatccg ggcgggccgc gcgggctggt
     3421 cggcaaagtg ggcgtcacgg tctcgcccgc caaccccgag cgcgtgtggg ccatcatcga
     3481 ggccgaggac ggcggcgtct tccgctccga caacggaggg cgcacttgga cgcgcacgaa
     3541 cgaacagcgc aacctccggc agcgcgcctg gtactacacg cgcatctacg ccgacaccga
     3601 gaacccggag gcggtctacg tcctcaacgt cggcttcttc aagtcgaacg acggcgggcg
     3661 caccttcagc cagatcagca cgccgcactc ggacaaccac gacctctgga tcgcgcccaa
     3721 cgatgccaac cggatgatcg aaggcaacga cggcggcgcc aacgtctcgg tcaacggcgg
     3781 gcgctcgtgg acggagcagg atcagccgac cgcgcagttc taccgcgtcg ccgtggacga
     3841 cgacttcccc taccacatct acggcgcgca gcaggacaac tcgaccgtca agatcgcctc
     3901 gcgcacgaac ggcttcggca tcggcgaatc ggactggcac gacgtgggcg gcggcgagtc
     3961 gggctggatc gcgccgcacc cgaaggactc cgacatcgtc tttgcaggct cctacggcgg
     4021 gtatctcacg cgctacgacc accgcacggg ccagcagcgc gccgtcaacg tctggcccga
     4081 aaacccgatg ggccacggcg cagagggcat gaagtaccgc ttccagtgga actacccgat
     4141 cctcttttcg ccgcacgaca catctaacgg aggcgtcctc tacgcggcgg gcgaccgcct
     4201 cttccgctcg accaacgaag ggcagtcgtg ggagattatg tcgcccgacc tgacgcgcaa
     4261 cgacaagtcg aagcaggggc cttcgggcgg gccgatcacg aaggacaaca cgagcgtcga
     4321 gtactacaac accatcttca cgttgatgga gtcgcccgtc acgaagggcg tcatctgggc
     4381 gggcaccgac gacggcctcg tccacgtcac gcgcgacaac gggaagacgt ggtcgaacgt
     4441 cacgccgcgc ggcatccccg agtggagtca gatcaactcc gtcgaagcct cgccgcacga
     4501 cgctgggagc gcttacgtcg ccgcgacgat gtacaagtgg gacgacttcc gcccctacct
     4561 ctacaggacg agcgactacg gcaggacgtg gaagaagatc gacaacggca tcccggcgag
     4621 cgccttcacg cgcgtcgt
//
LOCUS       DBNGOH010000573         4570 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1918182, whole genome shotgun sequence.
ACCESSION   DBNGOH010000573 DBNGOH010000000
VERSION     DBNGOH010000573.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4570)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4570)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4570
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1918182"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            339..2570
                     /locus_tag="AC159P_11075"
     CDS             339..2570
                     /locus_tag="AC159P_11075"
                     /inference="COORDINATES: protein motif:HMM:NF022113.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpqB family beta-propeller domain-containing
                     protein"
                     /protein_id="HIR3696033.1"
                     /translation="MNGQNHYIYEFGPFRLDAQKRLLRREGEVVPLKPKAFETLLVLV
                     EGCGRVVEKDELMRRVWPDTAVEEGNLTFNISSLRKALGDDPRRHEYIVTIPGEGYRF
                     VAGVRATFDELVVHESTSITVEEEEEAAPTAEGDGHPNTPPAAPAPRPHGLADAARSA
                     RRRRGLLIVVAALSVVGAATAFGTYRLVSQRRPPATPFGAIGISRLTTTGKVTHAAIS
                     PAGEYVAYVTAGADGDSLWVRHVTAPTSVLIAGPAVTEYVWVTFAPDGGSVYYLALDR
                     DKGETALYRVPVLGGPSVMTAYDVGPVGFSPDGSRTAFIRMRGDESHLVLADADGGNG
                     RTLAVRRRPEYFRMDWNAPAWSPDGRTIAAPARLSDGGGGYETVVGVSVAEGAQAQLT
                     PARWSCVNQPAWLADGSGLLLTARESESAPERVWHVTPQGGAVRRVTHDLNDYHDLSL
                     TAHAKRLAVVQVHAVSSIWVAPDGGAGRPRQVGSEAGSVEVVAWSPDGRIVYRSNAEA
                     GADIWSVGTDGSTPKQLTVGAQASRGLAVTPDGRHIIFVSDRAGHFNVWRVDADGGNL
                     TQLTFGEGEFYPHVSPDGRWVVYQRGEIRPTLWKVPTGGGEPVQLTEPRANRPAVSPD
                     GRLIAYHYLDTDVDRMRWGIGVVPSEGGGRLKRFDFAPTVTQRYVRWSPDGRSIAYPN
                     SPGGLSDIWSQPLDGGPPKRLTDLKAEHILAFDWSRDGRSLAIVSVVETSDVVLIDDS
                     VLR"
     gene            complement(2962..3102)
                     /locus_tag="AC159P_11080"
     CDS             complement(2962..3102)
                     /locus_tag="AC159P_11080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696034.1"
                     /translation="MAERLEVDCKTLAEEIGAYEGVADQLRAKLAAKERTREIARQFA
                     DY"
     gene            complement(3133..3360)
                     /locus_tag="AC159P_11085"
     CDS             complement(3133..3360)
                     /locus_tag="AC159P_11085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696035.1"
                     /translation="MTEPVECSCGRVMAEEVEKLVWDKVTSFFRLPEVVEAEVRRIME
                     DWPTDTITDDLAGVEKELAKSRKLYEKLYGK"
     gene            3711..4091
                     /locus_tag="AC159P_11090"
     CDS             3711..4091
                     /locus_tag="AC159P_11090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696036.1"
                     /translation="MSHKIEKPFIARFIDAEEKVLLELTNTTGEVLRGVEILAVFLTD
                     QGTPGGGPSRSHIKFDSLKSVRPKEQAVLSHRTWIDGKPADADHDQLERLKVVVGEVK
                     PYVLDISWEDGEGKTRYQRIPVGH"
     gene            4102..>4570
                     /locus_tag="AC159P_11095"
     CDS             4102..>4570
                     /locus_tag="AC159P_11095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696037.1"
                     /translation="MMLLAEFEERRHRARRALDGATSDNIVELCKQYLALLAEYRAEL
                     YKLPDTLDIKQWSGACIWEDVGSVRKAVRAAIENTVRERNGTEALLRSFTSLSGYEAV
                     ENLNRQKYKGHDDWELRAGGVARFRGDIAEERMIVLEAVDTAGLLRREEHIARY"
BASE COUNT          828 a         1512 c         1469 g          761 t
ORIGIN      
        1 cacgctcacc atcccgacct ccgccccgca gcccgcgcac cacccgggcc gcgcgccgcc
       61 ccggctgcgg aagacgaagg tgcgttcagt cttgacggtc actctcctga cgcgcttgga
      121 cttgtccata ttcagtgagc ggtgagcagt gagtaccaga cgcggataaa ccgctcaccg
      181 ctcccttttt tttccctgcg gggcgcgcta aaatgagcgc gctttcaagg tctccacctg
      241 ccccggactg tcttcggttc gcggcgcacg gtacagagaa agggcaggga agtcctttat
      301 tcacctcgta gaatttctta gaagttctta acgcgacggt gaacgggcag aatcactaca
      361 tctacgagtt cggccccttc cgcctcgacg cgcagaagcg cctgctccgg cgcgagggcg
      421 aggtcgtgcc gctcaagccg aaggcgttcg agacgctcct cgtgctggtt gaaggatgcg
      481 ggcgggtcgt ggagaaggac gagctgatgc ggcgcgtctg gccggacacc gcggtcgagg
      541 aggggaacct caccttcaac atctcttccc tgcggaaggc gctcggcgac gacccgcgcc
      601 gccacgagta catcgtcacc atcccgggcg agggctaccg gttcgtggcg ggcgtgcgcg
      661 ccaccttcga cgagctggtc gtgcacgaga gcacgagcat tacggtcgag gaggaggagg
      721 aggccgcccc gaccgccgaa ggtgacgggc acccgaacac gccgcccgcc gcccccgccc
      781 cgcggccgca cgggctcgcg gacgccgccc ggagcgcgcg gcgcaggcga ggcctgttaa
      841 tagtcgtcgc ggcgctgtcg gtcgtcgggg cggccaccgc tttcggaacc tatcggctcg
      901 tcagccagcg ccgcccgccc gccacgccct tcggcgcgat aggcatctcg cgcctcacca
      961 ccaccggcaa agtcacgcac gcggccatct ccccggccgg cgaatacgtc gcctacgtga
     1021 cggcaggtgc ggacggcgac agcctctggg tcaggcacgt caccgcgccg acgagcgtgc
     1081 taatcgccgg gccggccgtg acggagtacg tctgggtcac gttcgcgccg gacggcggct
     1141 cggtctacta cctggctctg gaccgcgaca agggtgagac tgcgctctac cgcgtgccgg
     1201 tcttgggcgg cccgtcggtc atgacggctt acgacgtggg accggtcggc ttctcccccg
     1261 acgggagccg gacggccttt atccggatgc gcggcgacga gagccacctc gtcctggcgg
     1321 acgccgacgg cgggaacggg cggacgctgg ccgtccgccg gcggccggag tatttccgga
     1381 tggactggaa cgcgcccgcg tggtcgcccg acgggaggac cattgccgcc cccgcccggc
     1441 tctcggacgg gggcggcggc tacgagacgg tcgtcggggt gagcgtggcg gagggggcac
     1501 aggcacagct cacccccgcg cgctggagct gcgtcaacca gccggcgtgg ctcgcggacg
     1561 ggagcggcct gctcttgacg gcgcgcgaga gcgagtccgc ccccgaacgg gtctggcacg
     1621 tcacgccgca gggcggcgcg gtcaggcgtg tgacgcacga cctgaacgac taccacgacc
     1681 tcagcctcac cgcccacgcg aaacggctgg cggtcgtgca ggtccatgcc gtctcgagca
     1741 tctgggtcgc gcccgacggg ggcgcgggcc gcccgaggca ggtcggttcc gaagccggct
     1801 cggtcgaagt ggtggcatgg tcgcccgacg gccgcatcgt ctaccgctcg aacgccgagg
     1861 ccggcgcgga catctggagc gtgggcacgg acggctcaac ccccaaacaa ctgacggtcg
     1921 gcgcgcaggc gagccgcggg ctggcggtga cgcccgacgg ccgtcacatc attttcgtct
     1981 ccgaccgcgc gggacatttc aacgtctggc gggtggacgc cgacggaggc aacctcacgc
     2041 agctgacctt cggggagggc gagttctacc cgcacgtcag ccccgacggg cggtgggtcg
     2101 tctaccagcg cggcgagatc aggccgacgc tctggaaagt gcccaccggc gggggcgagc
     2161 ccgtccagct gaccgagccg cgcgcgaacc gccccgccgt ctcgcccgac ggacgtctga
     2221 tcgcttacca ctacctcgac accgatgtgg acaggatgag gtggggcatc ggggtcgttc
     2281 cgtccgaagg gggcggccgc ctgaagcgtt tcgacttcgc gccgacggtg acgcagaggt
     2341 acgtccgctg gtcccctgac ggtcgctcga tcgcctaccc gaacagcccc ggcgggctct
     2401 ccgacatctg gtcccagccg ctcgacggcg gcccgcctaa gcgactgacc gacctgaagg
     2461 ccgagcacat cctcgccttc gactggtcgc gcgacgggcg ctcactcgca atcgtcagcg
     2521 tcgtcgagac gagcgatgtg gttctcattg acgactccgt tttgagatga tgagtgcggg
     2581 gccgcacgcc gcgtcagtcc gcggcgtcac ccgtcgagcc gccccactca cggaagttac
     2641 gggtcgtata tacatggggg acaaaatgct cctacccaac aatgccaggg cggagctgcc
     2701 caccaaagta ccttgagagt cttcggcgac taacgagaat tcaagagtca gaccggtttt
     2761 gaaatggccc gaactgcttc cagtttggct tgaaactgaa cttttgattt cagggcagat
     2821 agctaaaatc gccccgagat ttgtttgcaa cttcagcgtg ccgttcgagt gagcgagcac
     2881 agtaacgcgc aagacggtga gcatctccct cttcttctcg aaggggatgc tcaccgtctc
     2941 aaacgcgccg tccagcttcc gtcagtagtc cgcgaactgc cgcgcaatct ccctggtgcg
     3001 ctccttagcc gcgagcttcg cgcgtagctg gtcggccacg ccctcgtaag ctccgatctc
     3061 ctccgccaga gtcttgcagt ccacctccag ccgctccgcc atcatgtcgt cctcatccgt
     3121 aagcgcagcc ctctacttcc cgtacagctt ctcgtagagc ttccggctct tcgcaagctc
     3181 tttctccacg ccggcgaggt cgtccgtgat cgtgtcggtc ggccagtcct ccatgatgcg
     3241 gcgcacctcc gcctcaacga cctccggcag ccggaagaaa gaagtgacct tgtcccagac
     3301 cagtttctcc acctcttccg ccatcacgcg cccgcacgag cactcgaccg gctctgtcat
     3361 gcgaccgccc ggacgcgcgg ctgaaagatg tgcgacaggc cgatcacgaa gaaggatacg
     3421 atggcgagtt tttggacggc cacttccata catgttcccc tcaacctgaa tcaagagata
     3481 gaggactcga ttcttaacag cccccacacg aacggcccgc cggaagcgga tcataagtgt
     3541 gccatcgcac agacggtcat gcgacggcgg catataatgt ttgtccctcg gcaggtgttg
     3601 aatgtgcctg tccggcgcag gccgccttta tagcttgcgt tcaacgggtg tggaaggagg
     3661 cggagtctct actccgccgc ctttcttctc gcgaaagaaa gttcacgacc atgagccaca
     3721 agatcgagaa gcccttcatc gccaggttca tcgacgccga agagaaggtg cttttagagt
     3781 tgacgaacac gaccggcgag gtgctcaggg gcgtcgagat tctggccgtc ttcctcacag
     3841 accaggggac gccgggcggc ggcccctccc ggtctcatat caaattcgac tctctcaagt
     3901 cggtcagacc caaagagcag gcagtcctgt cccaccgcac ctggatcgac ggtaagccgg
     3961 cggacgcaga ccacgaccag ttggaacgtc tgaaagtggt ggtgggtgaa gtcaaaccgt
     4021 acgtgctgga tatttcgtgg gaggacgggg aagggaaaac gcgctaccag cggatacccg
     4081 tgggacactg accggctcat catgatgtta ctcgcggaat tcgaagagag aaggcatcgc
     4141 gcccggcgcg ctctcgacgg ggcgacgagc gataacattg tcgagctttg taaacagtat
     4201 ctcgcgctgc tcgccgagta ccgcgccgag ctttacaaac ttcccgacac gctggacatc
     4261 aagcagtggt caggagcctg catttgggaa gatgtcggca gcgtcaggaa agcagtccgc
     4321 gccgcgattg agaataccgt cagagagcgc aacgggactg aggcgctcct ccgctctttc
     4381 acttcgctaa gcggctacga ggccgtggag aacctcaaca ggcagaaata taagggacat
     4441 gacgattggg aactgagggc cggaggggtc gcccgatttc gcggtgacat cgcggaagag
     4501 aggatgatcg tcctggaagc cgtggacacc gccggcctgc tgaggcgcga ggaacacatc
     4561 gcccggtatg
//
LOCUS       DBNGOH010000574         4891 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2834309, whole genome shotgun sequence.
ACCESSION   DBNGOH010000574 DBNGOH010000000
VERSION     DBNGOH010000574.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4891)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4891)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4891
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2834309"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="AC159P_11100"
     CDS             <1..447
                     /locus_tag="AC159P_11100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006623133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HIR3696038.1"
                     /translation="DAEPFVLFELAGTTYGVRSRFVQQMEMVEHITPVPNAAPAVEGV
                     VLARGQVIPALNLRVRFGFERVPFDLRSRLMVVNKGGRTLGLIVDTAREFLRIPEAAV
                     EPPPEAITGLSGKYLEGIATIKDRMILVLDLDEVIDIGEQQPGVGG"
     gene            460..2655
                     /locus_tag="AC159P_11105"
     CDS             460..2655
                     /locus_tag="AC159P_11105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017721263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HIR3696039.1"
                     /translation="MATRMSNAAGLGASAGLVREQATRVADAAHEIARLANEVNEGTE
                     AQIGSLDAAVSLSNEMVASLGETTTQAESIATSAEELVSSVNETAASIEQVGANSASL
                     AASINETAASIEESARSIESVTAGAQEMATSAEEVTSAMNEIAASIKSVSDDTEALAA
                     SVNQTAVSMEQVARSIDSLAGNADDLTVSAEETASSINEMAASVEEVSAMSENLASTV
                     EEVSTSMEEMARSVQGVAQNTERITEAATGAATSAAEMERSLRSVAAMTKRAEEIADR
                     VSREAEQGGAAARKSIQGMGRVRASMEQSASVIREMGKRSNEIGGIVNTINVIAERTN
                     MLSLNASIEAARAGEAGRGFAVVAEEIRNLADRSAQATADIAAIIKALQDVVQEAVAA
                     SGEGVRIAEESGRLVEEGSEGLQRILSGVGETTQIVGQIARATEEQLGAGAHVVAAVE
                     TTATQAKQSTVATAEQAKAAQEIVRAAAQMRKTAKEVSQAMGEQGRAAREIIKAAQST
                     SAVAAQVRQASGEQAAAAKQVAQAVESMRRGAASTSRALEEPATAGEQVSKESERLAR
                     MIAGVSRAMGEQAAAATQITVAAKSMRQQSEQVAKAVDEQARAARDMSSATAGISKEV
                     GLITRSNRQHLSSSEQVLGTLNDIRQITERNARGVEATLSGTTGLIERARQLNAIVDG
                     AEAGQLGENGAPPAGARPRPNGRPRRPKKGAAAAEAAGTGGEGRPDGEQ"
     gene            2713..>4891
                     /locus_tag="AC159P_11110"
     CDS             2713..>4891
                     /locus_tag="AC159P_11110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017721262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sister chromatid cohesion protein PDS5"
                     /protein_id="HIR3696040.1"
                     /translation="MGSLTSSDNPIGVFTTDAGLVVRIWDAALARLTGIPADAACGKP
                     LAELLPHLKESRLLARFRRVLDEGVVEVLAPAFHHYLIPCAPSRPSKHFDKMQQRVTI
                     APLRENERTIGALVTVEDVTARIERERGLSEQLAHADEGERLSAAQALAGDETLVEPA
                     RPLIAALGDESWRVRRVAVEGLARRAAPDAIAALLASVRENHHDLGLLNSALQVLAMS
                     DVDTLSPLVEFLHDEDADLRMQAALALGEQRDVRATPALLGALGDADTNVRYHAIEAL
                     GKLRAAEAADSLASVAETRDFFLAFPALDALTRIGDARVAPRVVPLLEDELLREAAAD
                     VLGRLGDEEAVAPLAALLNRPGAPARVVAQSLAALHERYEKLYGEGAYIADLSRAAIN
                     PTGARTLLDALNESDAGGLRSLALVVGWLEGAAVERALTRLLGQRDARAEVIEALVRH
                     GSRVTDLLMEQLGSEDLEIRQSAVVALGRIGDARAAPALVEVLGEDPELLIHAADALA
                     KIGDPRAFDALLGLVGDPNAAVRQAVVGALNSLGSPEMPGRVLPLLEDEDPNVRESAV
                     KIAGYFGYPECADLLLERCRDREERVRRAAVEHLPFLEDERASPALVRALRDETPKVR
                     AAAAGAMAQAEGLEVSRCLMEALADEDSWVRYFAARSLGRRMVVESMDALAGLARDDK
                     SNHVRIAAVEALGHVGGQSAARFVAPLVKSDEPDIVRAAVKALG"
BASE COUNT          830 a         1665 c         1780 g          616 t
ORIGIN      
        1 gacgccgaac ccttcgtcct cttcgagctg gcggggacga cttacggcgt ccgcagccgc
       61 ttcgtgcagc agatggagat ggtcgagcac atcacgccgg tgccgaacgc ggcccccgcg
      121 gtcgagggcg tcgtgctcgc gcgcgggcag gtcatcccgg cgctcaacct gcgcgtgcgc
      181 ttcggcttcg aaagagttcc tttcgacctg cgctcgcgcc tgatggtggt caacaaaggg
      241 gggcgcacgc tcgggctgat cgtggacacg gcgcgggagt tcctgcgcat ccccgaggcc
      301 gccgtcgagc cgccgccgga ggccatcacg ggcctcagcg ggaagtatct ggaagggatc
      361 gcgacgatca aggacaggat gattctggtt ctcgatctgg acgaagtgat cgacatcggc
      421 gaacagcagc ccggagtcgg cggttaacag gagaggcaca tggcgacacg gatgagcaat
      481 gcggcgggcc tcggcgcgag cgccggactc gtgcgcgaac aggcgacgcg ggtggcggac
      541 gcggcccacg agatcgcgcg gctcgcgaac gaagtgaacg agggcacgga ggcgcagata
      601 ggctcgctcg acgcggcggt cagcctctcg aacgagatgg tggcctcgct cggcgagacg
      661 accacgcagg ccgagtcgat agccacctcc gccgaagaac tcgtctcctc ggtcaacgag
      721 acggcggcct ccatcgagca ggtcggcgcc aactcggcga gcctcgccgc ctcgatcaac
      781 gagacggcgg cctccatcga ggagtcggcc cgctccatcg agagcgtcac cgccggcgcg
      841 caggagatgg cgacctccgc cgaggaggtg acgagcgcga tgaacgagat cgcggcctcc
      901 atcaagagcg tcagcgacga caccgaagcc ctcgccgcgt cggtcaacca gaccgccgtc
      961 agcatggagc aggtggcgcg ctccatcgac agcctcgccg ggaacgccga cgatctgacc
     1021 gtctccgccg aagagaccgc ctcgtccatc aacgagatgg ccgcctccgt cgaggaggtc
     1081 tcggcgatgt cggagaatct cgcctcgacc gtcgaggagg tttcgacctc gatggaggag
     1141 atggcgcgct ccgtccaggg cgtggcgcag aacaccgagc ggatcaccga ggcggcgacc
     1201 ggcgcggcca cgagcgccgc cgagatggag cgctcgctgc gctcggtcgc cgcgatgacc
     1261 aagcgcgccg aagagatcgc cgaccgcgtc tcgcgcgagg cggagcaagg cggcgcggcg
     1321 gcgcggaagt ccattcaggg catgggccgc gtccgcgcct cgatggagca gtcggcgtcg
     1381 gtcatccgcg agatgggcaa gcgctcgaac gagatcggcg gcatcgtcaa caccatcaac
     1441 gtcatcgccg agcgcacgaa catgttgtcg ctgaacgcgt ccatcgaagc ggcgcgcgcc
     1501 ggcgaggcgg gtcgcggctt cgccgtcgtc gccgaagaga ttcgcaacct cgccgaccgc
     1561 tcggcgcagg ccaccgccga catcgccgcc atcatcaagg cgttgcagga cgtggtgcag
     1621 gaggccgtcg cggcgtcggg cgagggggtg cgcatcgccg aagagagcgg acgcctcgtg
     1681 gaggaagggt cggaggggct gcaaagaatc ctgtccggcg tgggcgagac cacacagatc
     1741 gtcggccaga tagcgcgcgc caccgaggag cagttgggcg cgggcgcgca cgtcgtggcg
     1801 gcagtcgaga cgaccgcaac acaggccaag cagtccacgg tcgccaccgc cgagcaggcc
     1861 aaggccgcgc aggagatcgt cagggcggcg gcgcagatgc gcaagaccgc caaggaggtc
     1921 agtcaggcga tgggcgagca ggggcgcgcc gcccgcgaga tcatcaaggc cgcgcagagc
     1981 acgtccgcgg tggccgcgca ggtgcgccaa gcctcgggcg agcaggcggc ggcggccaaa
     2041 caggtcgcgc aggcggtcga gtcgatgcgc cgcggcgcgg ccagcacctc gcgcgcgctc
     2101 gaagagccgg ccacggcggg cgaacaagtc tcgaaagagt cggaacgtct ggcgcggatg
     2161 atcgcgggcg tgagccgcgc gatgggggaa caggccgccg ccgcgacgca aatcaccgtc
     2221 gccgcaaaga gcatgcgcca gcagtccgag caggtcgcca aagccgtgga cgagcaggca
     2281 cgggccgccc gcgacatgtc ctccgccacc gccggcatct ccaaagaggt cggtctcatc
     2341 acgcgctcga accgccaaca cctatcctcc tccgagcagg tcttgggcac gcttaacgac
     2401 atccggcaga tcaccgagcg caacgcgcgg ggagtcgaag ccactttgag cggcaccacc
     2461 ggtttgatcg agcgggcacg gcaactcaac gcgatcgtgg acggcgcaga ggcgggtcaa
     2521 ctcggcgaaa acggcgcgcc gcccgcgggc gccaggccgc gccctaacgg aagaccgcgg
     2581 cgccctaaga aaggggccgc cgcggcggag gccgccggga cgggcgggga ggggcggcct
     2641 gacggagagc aatgaaatat tttttcgcgt ccgcacggat acgcgtgtgg ctgagacggt
     2701 gacgtgaccg ggatgggcag cctgacctct tcagacaatc caatcggagt ttttaccacc
     2761 gacgcgggtc tcgtcgttcg aatctgggac gcggccctcg cgcgcctgac gggcatcccc
     2821 gcggacgcgg cctgcgggaa gccgctggcc gagttgctcc cccacctgaa agagagccgg
     2881 ctgctcgcac gcttccggcg ggtgctggac gaaggggtcg tggaagtcct cgcgccggcg
     2941 ttccaccact acctgatccc ctgtgccccg tcccgcccct cgaagcattt cgacaagatg
     3001 cagcagcgcg tgaccatcgc gcccctgcgc gagaacgaac ggacaatcgg cgcgctcgtc
     3061 acggtcgaag acgtgacggc gcggatcgag cgcgagcgcg gcctctccga acaactcgcc
     3121 cacgccgacg agggcgaacg actcagcgcc gcgcaagccc tcgcgggcga cgagacgctc
     3181 gtcgaacccg cgcggccact catcgccgcg ctcggggacg agagctggcg cgtccggcgc
     3241 gtggccgtcg aggggctggc gcgccgcgcc gcgccggacg ccatcgccgc gctcctcgcc
     3301 tccgtgcgcg agaaccatca cgacctcgga ctgctcaaca gcgcgctgca agtgctcgcg
     3361 atgagcgacg tggacacgct ctcgccgctc gtcgaattcc tgcacgacga agacgcggac
     3421 ctgcgcatgc aggcggcgct cgccctcgga gagcagcgcg acgtgcgcgc cacgcccgcg
     3481 ctgctcggcg ccctcggcga cgccgatacc aacgtgcgct atcacgcgat cgaggcgctg
     3541 gggaagctgc gggcggctga ggccgcggac tcgctcgcgt ccgtcgccga gacgcgcgat
     3601 ttctttctgg ccttcccggc gctcgacgcc ctgacgcgca tcggcgacgc gcgcgtcgcc
     3661 ccgcgcgtcg tgccgctgct ggaagacgag cttttacgcg aggcggctgc cgacgtgctc
     3721 ggcaggctcg gcgacgaaga agcggtcgcg ccgctcgccg cgctgctgaa cagaccgggc
     3781 gcgcccgcgc gggtcgtcgc gcaatcgctc gccgcgctcc acgaacgtta cgagaagctg
     3841 tacggcgagg gcgcgtacat cgccgacctg agccgcgcgg cgatcaaccc gacgggcgcg
     3901 cggactctgc tcgacgcgct gaacgagtcg gatgcgggcg ggctgcgctc cctcgcgctg
     3961 gtcgtcgggt ggctggaggg cgcagcggtc gagcgcgccc tgacgcgcct gctcgggcag
     4021 agggacgcgc gcgcagaggt catcgaagcg ctggtgcgac acggctcgcg cgtgacggac
     4081 ttgctgatgg agcagctcgg gtcggaagac ctcgaaatca gacagtcggc ggtcgtcgcc
     4141 ctcgggcgca tcggcgacgc gcgcgccgcg cccgcgctgg tcgaagtcct cggcgaagac
     4201 ccggagctgt tgattcacgc ggcggacgcg ctggcgaaga tcggcgaccc gcgcgcgttc
     4261 gacgcgctgc tcggtctggt cggagacccg aacgcggcgg tcagacaggc cgtcgtcgga
     4321 gcgctcaact cgctcggctc gcccgagatg ccggggcgcg tgctgccgct gctcgaagac
     4381 gaagacccga acgtgcgcga atcggcggtc aagatcgccg ggtacttcgg ctaccccgaa
     4441 tgcgccgacc tccttctgga gcgatgccgc gaccgggaag agcgcgtgcg ccgcgcggcg
     4501 gtcgaacacc tcccgtttct cgaagacgaa cgcgcaagcc ccgcgctcgt gcgcgcgctg
     4561 cgcgacgaga cgccgaaagt gcgcgcggcg gcggcgggcg cgatggcgca ggccgagggc
     4621 ttggaagtct cgcgctgttt gatggaggcg ctggcggacg aagattcgtg ggtgcgttac
     4681 ttcgccgccc gttcgctcgg taggcgcatg gtcgtcgaga gcatggacgc gctggcgggg
     4741 ctggcgcggg acgataaatc gaatcacgtg cgcatcgcag ccgtcgaggc gctgggacac
     4801 gtgggcgggc agagcgcggc ccggttcgtc gcccccctcg tcaaatccga cgagccggac
     4861 attgtgcggg ccgccgtcaa agcgcttgga c
//
LOCUS       DBNGOH010000575         5129 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4850388, whole genome shotgun sequence.
ACCESSION   DBNGOH010000575 DBNGOH010000000
VERSION     DBNGOH010000575.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5129)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5129)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5129
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4850388"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..445)
                     /locus_tag="AC159P_11115"
     CDS             complement(<1..445)
                     /locus_tag="AC159P_11115"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3696041.1"
                     /translation="MIIEQVTVTAFQQHTRVLGCEETKRCVCVDPGDETEEIAETIER
                     RGLELQAIALTHAHLDHVGGVAALKRRMPRAEVIIHREDEPLYRALPEQPAWIGVPRA
                     QWAALGFEFEEPPPVERYWEDGETYRVGRLEFRVLHCPGHTPGHVV"
     gene            559..2967
                     /locus_tag="AC159P_11120"
     CDS             559..2967
                     /locus_tag="AC159P_11120"
                     /inference="COORDINATES: protein motif:HMM:NF015983.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HIR3696042.1"
                     /translation="MPLSARSASLAEVLDEMTAEGAAELTERAADGALRCSACGHRCL
                     IGEGKRGICKVRFNEGGRLRVPANYVAALQCDPTEKKPFFHVLPGSDTLTFGMLGCDL
                     HCSYCFTGDTPVVTDRGPLTFEEAFNSAARVERHTDAEIAYHADLRAVAASGTLRRVR
                     GIFKHAYRGRLAVIRPYYLPALRCTPDHRVYATEDPAIAPRLIQAQLLTDRHYLAVPR
                     RYAFSSAQVVDVASVLGGHRITYKVPWGVSAADREAIAEATARGETSRRIGAALGKGT
                     SYIRHVRSKAARGRAVDARSSGPLVENGLLGFPNEHRPGIPLSVALDEDFARLLGLYC
                     AEGSVCSDKTRPNSHVLNFSFSPAEEHLAEEAVRLLRERLGLKAAIVRRATTLAVAAG
                     KSSAALLFEALAGKRSGEKRVPAMIFDAHRHIVRAFLDAYVEGDGHRYGKGKVSVTTV
                     SRQLAYGVAWLALKLGHLPSVYDAEVAERGFVQGRPVKRSPHQYTVVWYEGVQPRRRA
                     LETDDFHLVPLREIAFADYDGDVYNMEVEEEHNYLAGFFAASNCQNWLTSQALRDDAA
                     GTAPRRVSAERLVELARECGAQVVGSSYNEPLITAEWAVEVFKRARPEGLRTAFISNG
                     NATPQVLDYLRPWTDCYKIDLKTMSDRGYRQLGGVVGNILDTVRMVRERGFWEEIVTL
                     VIPGFNDSEDELRRAADFIASVSPDIPWHVTAFHQDYRMTENANTTAEQLVRACETGR
                     AAGLRYVYAGNLPGRVGRWENTYCPACDALLVERHGYRILQMNVGADGLCPRCDARIP
                     GVWS"
     gene            complement(3086..3997)
                     /locus_tag="AC159P_11125"
     CDS             complement(3086..3997)
                     /locus_tag="AC159P_11125"
                     /inference="COORDINATES: protein motif:HMM:NF021640.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050135 - NADP+ nucleosidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HIR3696043.1"
                     /translation="MSIARRIYISVPGDSRLTESQQLLKWALIDKVSELGYTPEMFYS
                     ERPQSGSLAHGRNWSFAECSAVLRRCVGAIIIGLPRHQFSTDDGPVRLPTEYAHIEGA
                     LAIDLDLPTFLLAEEGVSDRGMFFPGGAHLITAIPSGADAAWTAAPKFQNALSIFVER
                     VRERRDIFLGYCGTSEGTAQNLKRFIELQIGATVLDWRTDFIEGRTIIEEIEEAARRT
                     SGGVFLFTKDDPLEGEEGLAAPRDNVVFEAGFFAHAKGRGRVLIVREDGAKMPADLGG
                     SIYVKLSDRTNIGPLETRLKRFLETAI"
     gene            complement(4118..5065)
                     /locus_tag="AC159P_11130"
     CDS             complement(4118..5065)
                     /locus_tag="AC159P_11130"
                     /inference="COORDINATES: protein motif:HMM:NF025887.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4440 domain-containing protein"
                     /protein_id="HIR3696044.1"
                     /translation="MKRTVLLAALLVVASACAAPAPSNNSTTSNRPTASTSPAPAASP
                     AASLVTEADALAREKQVYDALKAKNYDAFAAFLADDQVYVSGSGVQDKATTLKGINVF
                     EPTEVTLSEVKLLNATKNLYVLHYKAAVKGSVNGKPLPPDFAERDSTAWVNRGGKWLV
                     VYHQETSIKEPPPMPTPGAGPPPTVVASPAASPAASPAAAAATATDMEQQVWDTLKRG
                     DFEAFAAFLAEDQLEVEPTGVYNKAQTVAAVKQFDFKGTTLSDFKETKLDSDATLVTY
                     MVKSPAKGFNPAGERHTTLWVNRGGKWLAVFHQGSEVGK"
BASE COUNT          836 a         1686 c         1711 g          896 t
ORIGIN      
        1 gcacgacgtg gcccggcgtg tggccggggc agtggagcac cctgaactcc agacgcccga
       61 cgcggtacgt ctcgccgtcc tcccagtaac gctcgacggg cggcggctcc tcgaactcga
      121 agccgagcgc cgcccactgc gcgcgcggca ctccgatcca cgccggctgc tcgggcagcg
      181 cccggtagag cggctcgtcc tccctgtgga tgatgacctc cgcccgcggc atccgcctct
      241 tcaaagccgc cacgcccccg acgtgatcca gatgcgcgtg cgtcagcgcg atggcctgca
      301 actccaaacc tcttctctcg atcgtctcgg cgatctcctc cgtctcgtcg cccggatcga
      361 cgcagacgca cctcttcgtc tcctcgcagc cgaggacgcg cgtgtgctgc tgaaaggcgg
      421 tgacggtgac ctgctcgata atcattcgcg cgtggcctgt ccttttccga agggttataa
      481 tgacgagtta aaaaacggct ctgctcacga agtatagacg cccccgccgc ccccgccaaa
      541 cgaggaggtt caacgaagat gcctctgagc gcgcgctccg cttcactggc cgaggtgctg
      601 gacgagatga ccgccgaagg cgcggcggag ttgaccgaac gcgccgcgga cggcgccctg
      661 cgctgctccg cctgcggcca ccgctgcctc atcggcgaag gcaagcgcgg catctgcaaa
      721 gtccgcttca acgaaggcgg gcgcctgcgt gtgcccgcca actacgtcgc cgcgctccag
      781 tgcgacccca ccgagaagaa gcccttcttc cacgtcctgc ccggctccga caccctcacc
      841 ttcggcatgc tcggctgcga cctccactgc tcttactgct tcaccggcga caccccggtc
      901 gtcaccgacc gcggcccgct gaccttcgaa gaggctttca actcagccgc gcgcgtcgag
      961 cgccacacgg acgcggagat tgcttaccac gcagacctgc gcgccgtggc cgcctccggc
     1021 acactccgcc gcgtgcgcgg aatcttcaag cacgcgtatc gcggacggct cgccgtcatc
     1081 cgtccctact atctgcccgc gctgagatgc actcccgacc accgcgtcta cgcgaccgaa
     1141 gacccggcca tcgccccccg gctgattcaa gcacaactgc taaccgacag gcattacctg
     1201 gccgtcccgc gccgttacgc tttctcctcg gcgcaggtgg ttgacgtggc gagcgtgttg
     1261 ggcgggcacc ggataactta caaggtgccg tggggtgtgt cggctgccga ccgcgaggcg
     1321 attgccgagg cgacggcgcg cggcgagact tcgcgccgga tcggcgccgc gctgggtaaa
     1381 ggtacgtctt acatccggca cgtccgcagc aaggccgcgc gggggcgtgc ggtggacgcg
     1441 cggtcgagcg ggcctctcgt cgaaaacggc ctgctcggat ttcctaacga gcaccgcccc
     1501 ggcatccccc tttccgtcgc gctcgacgaa gacttcgcgc ggctgctcgg cctctattgc
     1561 gccgaagggt ctgtctgctc cgacaaaacg cgcccgaaca gccacgtcct caacttcagc
     1621 ttctcacccg ccgaagaaca tttggccgaa gaggccgtcc gcctgctccg cgaacgtctg
     1681 ggcctgaagg cggccatcgt gaggcgcgcg acgacgctgg cggtcgccgc gggcaaatcg
     1741 tccgcggccc tcctgttcga agcgctggcg ggaaaacgct ccggcgaaaa gcgtgttccg
     1801 gcgatgatct tcgacgcgca ccgccacatc gtccgcgcgt tcctcgacgc gtacgtggaa
     1861 ggtgacgggc accgctacgg caaaggcaaa gtcagcgtca cgaccgtctc ccgccaactg
     1921 gcctacggcg tcgcgtggct cgcgctgaag ctcggccact tgccgtccgt atacgacgcg
     1981 gaggtcgcgg agcgcggctt cgtgcagggc cgcccggtga agcgctcgcc gcaccagtac
     2041 acggtcgtct ggtacgaagg cgtgcagccg cggcgcaggg cgttggagac agacgacttc
     2101 catctcgtcc cgctccgcga aatcgccttc gccgactacg acggcgacgt ttacaacatg
     2161 gaagtcgaag aggagcacaa ctacctcgca ggcttcttcg cggcgtcgaa ctgccagaac
     2221 tggttaacgt cgcaggcgct gcgcgacgac gcggcaggga cggcgccgcg tcgtgtttca
     2281 gccgaacgtc tcgtcgagct ggcgcgcgag tgcggggcgc aggtggtggg gtcgagttat
     2341 aacgagccgt tgattacggc ggagtgggcg gtcgaagttt tcaagcgggc gcggcccgag
     2401 gggctgagga cggcgttcat ctcgaacggg aacgcgacgc cgcaggtgtt ggattacctg
     2461 cgcccgtgga cggactgcta caagatcgac ctgaagacga tgagcgaccg cggctacagg
     2521 cagttggggg gcgtggtggg caacatcctc gacaccgtcc ggatggtgcg cgagcgcggg
     2581 ttctgggagg agatcgtgac gctggtcatc cccggcttca acgactcgga ggacgagctg
     2641 cggcgggccg ccgacttcat cgcctcggtc tcgcccgaca tcccctggca cgtgacggcc
     2701 ttccaccagg actaccggat gacggagaac gcgaacacga ccgccgaaca actcgtgcgc
     2761 gcgtgcgaga cggggcgcgc tgccgggctg cgctacgtct acgcgggcaa cctgccgggc
     2821 cgcgtcgggc gctgggagaa cacctactgc ccggcctgcg acgcgctcct cgtcgagcgc
     2881 cacggctacc gcatcctgca aatgaacgtc ggcgccgacg gcctgtgccc gcgctgcgac
     2941 gcgcggattc cgggcgtctg gtcgtgagaa cgcccggcct cactcgccgc gccttcaacc
     3001 cgcaaaccac cccgcagcta acggcaggag gcaggctgaa tcgccggagg gcgcctgccg
     3061 tcgtcgggcg acgccggcct ctgtttcaga ttgccgtctc caggaaacgc ttcaaacgag
     3121 tttccagggg gccgatgttg gttcgatccg acagcttcac gtagatgctc cccccgaggt
     3181 ctgcgggcat ctttgccccg tcttctctga cgatcaaaac tcttccgcgc cctttcgcgt
     3241 gagcaaagaa acccgcctcg aagacgacgt tgtcgcgggg ggcggccagt ccttcttcgc
     3301 cttcgagggg gtcgtccttc gtgaacagaa agacgccgcc gctggtacgg cgtgccgctt
     3361 cctcgatctc ttcgatgatt gtgcggccct cgataaaatc ggtgcgccag tcaagtacgg
     3421 tggcgccgat ctgtagctcg atgaatcgtt tcaggttttg tgcggtgcct tcggacgtac
     3481 cgcagtaacc caggaatata tcgcggcgct cgcgaacgcg ctcgacgaat atcgaaaggg
     3541 cgttctggaa cttcggcgcc gccgtccaag ccgcgtccgc gccggaaggt attgcggtta
     3601 tcaggtgcgc gccgccgggg aagaacatgc cgcggtcgga gacgccctct tcagccagca
     3661 gaaaagttgg caggtcgagg tctatggcga gtgcgccttc gatgtgggcg tattcagtcg
     3721 gaaggcgcac aggcccgtcg tccgtagaga actgatggcg tgggaggccg atgatgattg
     3781 cgccgacgca gcgccgtaac acggcggagc attcggcaaa gctccagttc cttccgtgtg
     3841 cgaggctccc cgactgcggg cgctcgctgt agaacatctc cggggtgtaa ccaagttcgg
     3901 acactttatc aatcaaagcc catttcaaca gctgctgcga ctcggtaagg cggctgtctc
     3961 cagggacgct gatatagatg cggcgtgcga ttgacatttg ttccctcgcg aaggttgatc
     4021 ggggtgtcgg tttgcggcac acactcgggc gcccgaccga ggactgagga tgaagaccat
     4081 gctttcacac agtcctcagt cctcagctct tctttgttta tttcccgacc tcgctgccct
     4141 ggtgaaagac ggcgagccat ttgccgccgc ggttgaccca gagggtggtg tggcgctcgc
     4201 ccgcggggtt gaagcctttg gcgggcgact tgaccatgta ggtgacgagc gtggcgtcgg
     4261 agtcgagctt ggtctctttg aagtcgctca aggtcgtgcc cttgaagtcg aactgcttga
     4321 cggcggcgac cgtctgcgcc ttgttgtaga cgccggtggg ttcgacctca agctggtctt
     4381 cggcgaggaa ggcggcgaag gcttcgaagt cgccgcgctt gagcgtgtcc cagacctgct
     4441 gctccatgtc ggtggcggtc gcggcggcgg cggcgggcga cgcggcgggc gaagcggcgg
     4501 gcgaggcgac gaccgtgggc ggcgggccgg cgccgggggt cggcatcggc ggcggctcct
     4561 tgatcgatgt ctcctgatgg tagacgacga gccacttgcc gccgcggttg acccaggctg
     4621 tggagtcgcg ctcggcgaag tccggcggga gcggcttgcc gttgacggaa cccttcacgg
     4681 cagccttgta gtgcagcacg tacaggttct tggtcgcgtt caagagcttg acttcgctca
     4741 gcgtcacctc ggtcggctcg aagacgttga tgcccttgag cgtcgtggcc ttgtcctgca
     4801 cgccgctgcc gctgacgtag acctgatcgt cggcgaggaa ggcggcgaag gcgtcgtagt
     4861 tcttggcctt gagcgcgtcg tagacctgct tctcgcgggc gagggcgtcc gcttcggtga
     4921 cgagcgaggc cgcgggcgac gcggcgggcg cgggcgacgt gctcgcggtc ggtcggttcg
     4981 aggtggtgga gttgttggaa ggcgccgggg cggcgcaggc ggacgcgacg accagcagcg
     5041 ccgcgagcag gacggttctt ttcatgacgg acactccgcg aggaagggat acacagagcg
     5101 cgtgctttct acgctaaacg gggcggcaa
//
LOCUS       DBNGOH010000576         3251 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_5033941, whole genome shotgun sequence.
ACCESSION   DBNGOH010000576 DBNGOH010000000
VERSION     DBNGOH010000576.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3251)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3251)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3251
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_5033941"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1751
                     /locus_tag="AC159P_11135"
     CDS             <1..1751
                     /locus_tag="AC159P_11135"
                     /inference="COORDINATES: protein motif:HMM:NF015655.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase family
                     protein"
                     /protein_id="HIR3696045.1"
                     /translation="RDPGLHAPHTLISLGRRADRGLVTERERTELSDAYTFLRTLEHR
                     VQMEHGLQTHLVPDDAPRRTAVARRMHFDGADALAGFDRALALHTSNVHAAFRRVFGP
                     EADDDATPDEGAAVPTHTPAGVGASAGGATDAERLSRVLRAGADESLNAKRSLALATR
                     VVAALDKSGAGVGLDEESLSALARLCGASEFLGEMMAANPSLIPALATPAEVVVARDH
                     RALMRAAVDAERSFRAELAALRRAWSRLLVEIGARDAAGELRLADSNRLQTSLAAASV
                     NVALLVARRELARRHGQLAAGPRLAVLGLGRLASGGMDLGSDLDLVLVYDPATAPPLK
                     GFTHDEAYARLAELTVAALSNMTRDGHLYRVDLRLRPDGKNGSLTSGGTAFINYLAER
                     ADVWEWLAHVKLRAVAGDQEYGATVERRARAAVHEAAARAGHERLRAETRRVRERLER
                     ERARRGGTDIKFGPGGMLDVYFAARFLQLRDRVPDEGHDRSTASTLARLREAASLDEE
                     TFRALGEGYALLRALDHRLRLLVGRSTRLPAAEDHPLLGDLARLSGYRSSTELLRDLA
                     ARMSAVRDAYERITRA"
     gene            complement(1761..>3251)
                     /locus_tag="AC159P_11140"
     CDS             complement(1761..>3251)
                     /locus_tag="AC159P_11140"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF019334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HIR3696046.1"
                     /translation="LGEGGMGTVYRARRLHIGDEVAVKVLHPKYVADADAVERFRREA
                     RSAAIIRHANVVTIHDFGEARASGAPAYLVMELVRGTSLRDLLRREGRLAHTRAVALM
                     RDICAGVGVAHREGVVHRDLKPDNVIVVPPTDEGEREAAKVVDFGLAKLRDMTLGPNL
                     TQTGAVMGTPHYMSPEQCRGDELDARADVYSLGAMLYEMLAGGPPFRSNSLAGLISKH
                     LHDEPPALPAGSAPPALEAVCRRALAKNPDERQPDANTLSRELRAALDAPPIAAPPIV
                     AAAPRSLGAPTQHLTPRSEEPTLPHAAATREKRPRRLKFVAAGLVALLACALVAAVAL
                     RYVGDDDERDARTTGSNAAEASVTPTQEDAGVAPAVEEDGAPEEIAPDASPSAAGGGG
                     LAGSWVGTYGPLGQRTTLTIKDGKGGRLSGVLEQGGVRVAFEGSFDASSRKVTMKETR
                     LLSGSEWSLGENRGEVSADGRRMSGTGSDPVGTQLGITYTWSFSKR"
BASE COUNT          411 a         1261 c         1125 g          454 t
ORIGIN      
        1 ggcgcgaccc ggggcttcac gccccgcaca cgctcatcag cttgggccgc cgcgccgacc
       61 gcggcctcgt caccgagcgc gagcgcacgg agctttccga cgcctacacc ttcctgcgca
      121 cgctcgaaca ccgcgtgcag atggagcacg gcctgcaaac acacctcgtc cccgacgacg
      181 ccccgcgacg cacggcggtc gcgcgccgca tgcacttcga cggcgcggac gcgctcgccg
      241 gattcgaccg cgcgctcgcc cttcacacct cgaacgtcca cgccgccttc cgccgcgtct
      301 tcggccccga ggccgacgac gacgcgacgc ccgatgaagg cgccgccgtt ccgacacaca
      361 cgcccgcggg tgtaggcgcg agtgcaggcg gggcgacgga cgccgagagg ctgtcgcgcg
      421 tcctgcgcgc gggcgccgac gagtctttga acgcgaagcg ctcgctcgcg ctcgcgacgc
      481 gcgtcgtcgc cgccctcgac aagtcgggcg cgggcgtcgg gctggatgaa gagagtctgt
      541 cggcgctcgc gcggctgtgc ggcgcgtcgg aattcttggg cgagatgatg gccgccaacc
      601 cttcgctcat ccccgcgctc gcgacgcccg ccgaagtggt cgtcgcgcgc gaccaccgcg
      661 cgctgatgcg tgcggcggtg gacgcggagc ggagcttccg cgcggagctt gcggccttga
      721 ggcgcgcgtg gtcgcgcctg ctcgtcgaga tcggcgcgcg cgacgcggcg ggcgagcttc
      781 ggctcgccga ctcgaaccgc ctacagactt cgctcgccgc cgcctcggtc aacgtcgcgc
      841 tcctcgtcgc gcgccgcgag ctggcgcgcc gccacggcca gctcgccgcg gggccgcgcc
      901 tcgccgtcct cggcctcggg cggctcgcca gcggcggcat ggacttaggc tccgacctcg
      961 acctcgtgct cgtctacgac cccgccaccg cgccgccgct caagggcttc acgcacgacg
     1021 aggcttacgc gcggctcgcc gagctgacgg tcgccgcgct ctcgaacatg acgcgcgacg
     1081 gccacctcta tcgcgtcgat ctgcgcctca ggcccgacgg caagaacggc tcgctgactt
     1141 ccgggggcac tgccttcatc aactacctcg ccgagcgcgc ggacgtgtgg gagtggctcg
     1201 cgcacgtgaa gctgcgcgcg gtggcgggcg atcaggagta cggcgccacg gtcgagcgac
     1261 gcgcgcgcgc cgccgtccac gaagccgccg cgcgcgccgg gcatgaacgc ctccgcgccg
     1321 agacgcgccg cgtccgcgaa cgcctcgaac gcgagcgcgc gcggcgcggc ggcaccgaca
     1381 tcaagttcgg gccgggcggc atgctcgacg tgtacttcgc cgcccgcttc ctccagctcc
     1441 gcgaccgcgt cccggacgag ggccacgacc gctccaccgc ctccacgctc gcgcgtctgc
     1501 gcgaagccgc ctcgctcgac gaagagacct tccgcgcgct cggcgaaggc tacgcgttgc
     1561 tgcgcgcgct cgaccaccgc ctccgcctcc tcgtcggtcg ctccacgcgc ctgcccgccg
     1621 ccgaagacca cccgctgctc ggcgacctcg ccaggctctc gggttatcga tcctcgaccg
     1681 agctgctgcg cgacctcgcc gcgcggatgt ccgccgtccg cgacgcctac gaacgcatca
     1741 cgcgcgcgtg agcttggccg ctaccgtttc gagaacgacc aagtgtaggt gatgcccaac
     1801 tgcgtgccga ccgggtcgct gcccgtcccc gacatcctgc gcccgtcggc tgagacctcg
     1861 cccctgttct cgccgaggct ccactcgctg ccgctcaaga gccgcgtctc tttcatcgtc
     1921 actttccgcg aagaggcgtc gaagctgccc tcgaaggcga cgcgcacgcc gccctgctcc
     1981 aacacgccgc tgagcctgcc gcccttccca tccttgatgg tgagcgtcgt ccgctgaccc
     2041 agcgggccgt acgtccctac ccaactgcct gccaaccctc cgccgcccgc cgccgaaggc
     2101 gacgcgtccg gcgcgatctc ctccggcgcg ccgtcttctt cgacggcagg cgctacgccc
     2161 gcgtcctcct gcgtcggcgt gacggaggct tccgcggcgt tcgagccggt cgtgcgcgca
     2221 tcgcgttcgt cgtcatcgcc gacgtatctc agcgcgacgg cggcgacgag cgcgcaggcg
     2281 agcagcgcga cgagaccggc ggcgacgaac ttcagcctcc gaggcctctt ctctcgcgtg
     2341 gccgcggcgt gcggcagcgt cggctcttcg gagcgcggcg tcaagtgctg cgtaggtgcg
     2401 ccgagcgagc gcggggcggc ggcgacgatt ggcggggctg cgattggcgg cgcgtcgagg
     2461 gccgcccgca gctcgcggct gagggtgttc gcgtcgggct gacgctcgtc cggattttta
     2521 gcgagcgcgc ggcggcagac ggcttcgagc gcgggcggcg cggagcctgc gggcagcgcg
     2581 ggcggctcgt cgtgcaggtg cttcgagatg agaccggcga ggctgttgga gcggaacggc
     2641 ggcccgcccg ccagcatctc gtagagcatc gcgcccagag agtacacgtc ggcgcgcgca
     2701 tccagctcgt cgccgcggca ctgctccggc gacatgtaat gaggcgtgcc catcacggcg
     2761 cccgtctgcg tcaggttcgg ccccagcgtc atgtcgcgca gcttggcgag gccgaagtca
     2821 accaccttcg ccgcctcgcg ctcgccctcg tccgtgggcg gcacgacgat gacgttgtcc
     2881 ggcttgaggt cgcggtggac gacgccctcg cggtgcgcga cgccgacgcc cgcgcagatg
     2941 tcgcgcatca gcgcgacggc gcgcgtgtgc gcgagccgcc cctcgcggcg cagcagatcg
     3001 cgcagcgacg tgccgcgcac cagctccatg acgaggtagg cgggcgcccc cgaagcgcgc
     3061 gcctcgccga agtcgtggat cgtcaccacg ttcgcgtggc ggatgatggc ggcggagcgg
     3121 gcctcgcgcc ggaagcgctc gacggcgtcg gcgtccgcga cgtacttcgg gtgcagcacc
     3181 ttgaccgcca cctcgtcgcc gatgtgcagg cggcgcgcgc ggtagaccgt gcccatcccg
     3241 ccctcgccga g
//
LOCUS       DBNGOH010000577         3970 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_817839, whole genome shotgun sequence.
ACCESSION   DBNGOH010000577 DBNGOH010000000
VERSION     DBNGOH010000577.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3970)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3970)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3970
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_817839"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(58..651)
                     /locus_tag="AC159P_11145"
     CDS             complement(58..651)
                     /locus_tag="AC159P_11145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696047.1"
                     /translation="MQQKLTAAIIVLALAALVAACGGAADNTTTNANMTTTATVNSNA
                     AGLPATGSEIMTETADGVTTETRIFKDPNSPVEKVVVTTRGGKRTARVYYRDKTVREL
                     PDNAVEGALVSTADKLEDAGGKVVDVSKDIGSEVGDKAEDVGDKPVSGAKKVGAEAAD
                     KAEDVGDKAVSGAKKAGSATVKGAKKAGSAVKDAVTP"
     gene            complement(718..1263)
                     /locus_tag="AC159P_11150"
     CDS             complement(718..1263)
                     /locus_tag="AC159P_11150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696048.1"
                     /translation="MSKLFLSVLFAFALVAGMSTQAVARQDSGVKQDAEKAVDKTVDT
                     TKKVGQEVGDKAEDVGDKTKSGAKKAGKATAKGAKKVGHEAADKAEDAAGKAKSVGED
                     AADKTVSGAKTVGAEAADKTEDAAGATVKGAKVAGSKAEAVGEKAADTTMDAKDTTVK
                     GAKKTGNWFKRGMRKIGGIFN"
     gene            complement(1446..1517)
                     /locus_tag="AC159P_11155"
     tRNA            complement(1446..1517)
                     /locus_tag="AC159P_11155"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1483..1485),aa:Gly,seq:ccc)
     gene            complement(1489..1923)
                     /locus_tag="AC159P_11160"
     CDS             complement(1489..1923)
                     /locus_tag="AC159P_11160"
                     /inference="COORDINATES: protein motif:HMM:NF023075.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group I intron-associated PD-(D/E)XK
                     endonuclease"
                     /protein_id="HIR3696049.1"
                     /translation="MLTGEKGNLSEARTLAAFVAAGYLVSLPFGSGHKYDFVVDDSTR
                     LLRVQCKTGRIKNGVLLFNAYSQSGNGSVKMSYRGLADLFAVLNPDDGNVYLVPVDEV
                     GVTDVSLRLVPTVNGQTQGVRWAASYILKPLVLSGSGAMVAQ"
     gene            complement(1997..3148)
                     /locus_tag="AC159P_11165"
     CDS             complement(1997..3148)
                     /locus_tag="AC159P_11165"
                     /inference="COORDINATES: protein motif:HMM:NF012488.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HIR3696050.1"
                     /translation="MLGAETIERLGKADWRRVRAEFPVAEHTAYLNSAGTGPVARGTA
                     EAAAQFYRDVAESGDRRWEEWAARRERARESVARLVNAEPDEIAFTTNTSSGMNLVVD
                     ALEGRGDVVSCELEFPVSTITWMHRRARVHLVKAIEGELRTEDVLGAMNEGTSVVCLS
                     HVQYSNGFRLDPEEVGKNKGRHAFVLNASQSAGVLPIDVKRMRVDALCATGHKWMLAG
                     FGSGFVYLSRELQNGSRPRAVSWMSVENPFGMRNDAYTLRADAAARAETGCPHFAGIF
                     ALGASTDHILQLGAADIERRALALNRRLTERLTGTGWEVLSPLKSEAARSAETLVRAD
                     NARRAVAHLAARGVAVSIKPEGFRVATHFFNDEDDIERLVAALAELRGS"
     gene            3239..>3970
                     /locus_tag="AC159P_11170"
     CDS             3239..>3970
                     /locus_tag="AC159P_11170"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014803.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase family protein"
                     /protein_id="HIR3696051.1"
                     /translation="MSSTAAPTAPAWAIPGYLDARDGRLHVGGADSLALAREHDTPLF
                     VFSEQRIRANVERLKAAAARVERPVRFFYASKANSNMAVLGTVRDAGIDAEVNSGGEL
                     FKALRAGFRPDQLVFNGTSKTVREIDEAVGAGIYSVNVDSVYEIELIEARARALGKRA
                     RVALRLVPEIGTRSHLGLQTALMTSKFGITSSEVLDAFRRALRSPELLHVSGIHIHVG
                     SQTPDVEPFAAAFKTMWEHLKTIHDE"
BASE COUNT          619 a         1443 c         1151 g          757 t
ORIGIN      
        1 agggacgacc gtcgcaagtg acggtcgtcc ctcttctttc cagcgcctga tttttaacta
       61 cggagtcacc gcgtctttaa ccgcgctgcc cgccttcttc gcgcccttca cggtggcgct
      121 gcccgccttc ttcgcgccag agacggcctt gtcgccaacg tcttcggcct tatcggcggc
      181 ctcggcgccg accttcttcg cgccggagac gggcttgtct ccaacgtcct cggccttgtc
      241 gccgacctcc gagcctatgt ccttgctcac gtcaacgacc ttcccgcctg cgtcttcgag
      301 tttgtcggcg gtcgagacca gtgcgccctc gaccgcgttg tcgggcaact ccctgacggt
      361 cttgtcgcgg tagtagacac gcgccgtgcg cttgccgccg cgcgtcgtca cgacgacctt
      421 ctccacgggg ctgttcgggt ctttgaatat gcgcgtctcg gtcgtcacgc cgtcggcggt
      481 ctcggtcatg atctccgagc cggtcgccgg caggcccgcc gcgttcgagt tcacggtcgc
      541 ggtcgtagtc atgttcgcgt tcgtcgtggt gttgtcggcg gcgccgccgc acgcggcgac
      601 gagcgccgcc agcgcgagga cgatgatcgc cgccgttaat ttctgctgca cctctgaagc
      661 acctccgtta aagctgttgg gccgacccct tgttcgacgc tcgaccggct gccttcctta
      721 gttgaagatg ccgccgattt tacgcatccc gcgtttgaac cagttgccgg tcttcttcgc
      781 gcccttcacg gtcgtgtcct tcgcgtccat ggtcgtgtcc gcggccttct cgccgacggc
      841 ctcggccttc gacccggcta ccttggcgcc cttcacggtc gcgcccgccg cgtcctcggt
      901 cttgtccgcg gcctcggcgc cgacggtctt cgcaccggag acggtcttgt ccgcggcatc
      961 ctcgccgaca ctcttcgcct tgccggcggc gtcctccgcc ttatctgcgg cctcgtggcc
     1021 gaccttcttc gcgcccttcg cggtcgcctt cccggccttc ttcgcgccgg acttcgtctt
     1081 gtcgcccacg tcctcggcct tgtcgccgac ttcctgcccc accttcttcg tcgtatcgac
     1141 cgtcttatcg acggccttct ccgcgtcctg cttcaccccg ctgtcctgtc gcgcgacggc
     1201 ctgcgtactc atccccgcga ccaacgcgaa cgcgaacagt accgataaaa ataactttga
     1261 catcacccct gccctcccct tctgcaacga aatcaaacac tttccccttt gcccaatcaa
     1321 gtcggcaact ccagtgccag accagcgcct cacccatcat tccctcctcg ccgcatagaa
     1381 aaaacggtcg ccgcgaacga cgaccgtcaa cttcaccacc aaactaattc caccgactta
     1441 cacaaagcgg gagacgggac tcgaacccgc aacccatagc ttgggaagct attgcgctac
     1501 cattgcgcca ctcccgctta agaccagcgg cttaagaatg taagacgccg cccagcgtac
     1561 cccttgcgtc tgcccgttga cggtcgggac gagcctgagt gaaacgtccg tcacgcctac
     1621 ttcatcaacc ggaactaaat aaacgttacc gtcatcggga ttgaggacgg cgaacaagtc
     1681 cgccagacct ctgtaactca tcttaaccga cccgttgccc gattggctat aggcgttgaa
     1741 aagaaggacg ccgttcttga tcctgcccgt cttacattgc actcgtaaca ggcgagtcga
     1801 gtcgtcaact acgaaatcat atttatgtcc cgagccgaaa ggtaaggaaa cgagataacc
     1861 ggcagcgacg aaagccgcca gggttctggc ttcactcaga tttcctttct ctcccgtaag
     1921 catcaacgac gcgcttccgt cgagttactc ccgcgaggcc gaaaaaaata tactcgaccg
     1981 cccgcgggcc gtcaaatcaa ctgccgcgca actcggcgag cgcggcgacg aggcgttcga
     2041 tgtcgtcttc gtcgttgaag aagtgcgtgg cgacgcggaa gccttcgggc ttgatggaga
     2101 cggcgacgcc gcgcgcggcg aggtgtgcga cagcgcggcg cgcattgtcg gcccggacga
     2161 gcgtttcggc ggagcgcgcg gcttcgctct tgagcggcga gaggacttcc caaccggttc
     2221 cggtcagtcg ctcggtcagg cggcggttga gcgcgagcgc gcggcgctcg atgtcggcgg
     2281 cgccgagttg gaggatgtga tccgtgctcg cgccgagcgc gaagatgccc gcgaagtgtg
     2341 ggcagccggt ctcggcgcgg gcggcggcgt ccgcgcgcag cgtgtaggcg tcgttgcgca
     2401 tcccgaaggg gttttcgacg ctcatccaac tgacggcgcg cgggcgcgaa ccattttgca
     2461 gctcgcgcga gaggtagacg aagcccgagc cgaagccggc gagcatccac ttgtggccgg
     2521 tcgcgcacag cgcgtccacg cgcatccgct tcacgtcgat gggcagcacg cccgccgact
     2581 ggctcgcgtt caggacgaag gcgtgacggc ccttattctt tccgacctcc tccgggtcga
     2641 gccggaagcc gttcgaatac tgcacgtggc tgaggcagac gacgctcgtc ccttcgttca
     2701 tcgcgccgag cacgtcttcc gtccgcagct cgccttcgat tgctttaact aaatgaacgc
     2761 gcgcgcgcct gtgcatccac gtgatcgtcg agaccgggaa ctccagctcg cacgagacca
     2821 cgtcgccgcg cccttcgagc gcgtccacga cgaggttcat ccccgaggag gtgttggtcg
     2881 tgaaggcgat ctcgtccggc tcggcgttga cgagtcgcgc gaccgattcg cgcgcgcgct
     2941 cgcgccgcgc ggcccactcc tcccagcgcc ggtcgcccga ctccgccacg tcgcggtaaa
     3001 actgggcggc ggcctccgcc gtcccgcgcg cgaccgggcc ggtgcccgcg ctgttgaggt
     3061 aggccgtgtg ctccgcgacg ggaaattccg cgcgcacgcg ccgccagtcc gccttcccga
     3121 gtctctcgat tgtctccgcc ccgagcaccc gcgcccctcc tgcccttgac gctaaccgac
     3181 gcgcccgact atcttcgctc acgttcagcc aaatcggtct cgccggagtt ttcgatcaat
     3241 gtcttcaacc gcggccccga cggcccccgc ctgggctatc cccggctacc tcgacgcgcg
     3301 cgacgggcgc ctccacgtcg gcggcgccga ctcgctcgcc ctcgcgcgcg agcacgacac
     3361 gccgctcttc gtcttctcgg aacagcgcat ccgcgccaac gtcgaacggc tgaaggccgc
     3421 cgccgcccgc gtcgagcggc ccgtcagatt tttttacgcc tcgaaggcca actccaacat
     3481 ggccgtcctc ggcaccgtac gcgacgcagg gatagacgcc gaggtgaaca gcggcggcga
     3541 gctgttcaag gcgctgcgcg cgggcttccg ccccgaccag ctcgtcttca acggcacgag
     3601 caagaccgtc cgcgagatcg acgaggccgt cggcgcgggc atctactcgg tcaacgtcga
     3661 ttccgtctac gagatcgaac tgattgaagc acgcgcgcgc gccctcggca agcgcgcccg
     3721 cgtcgcgctc cgcctcgtcc ccgagatcgg cacgcgctcg cacctcggcc ttcagacggc
     3781 cttgatgacc tcgaagttcg gcatcacctc aagcgaagtc ctcgacgcct tccgccgcgc
     3841 gctgcgctcg cccgagctgc tccacgtctc cggcatacac atccacgtcg gctcgcagac
     3901 gcccgacgtg gagcctttcg ccgccgcctt caagacgatg tgggagcacc tgaagacgat
     3961 tcacgacgag
//
LOCUS       DBNGOH010000578         2333 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1369550, whole genome shotgun sequence.
ACCESSION   DBNGOH010000578 DBNGOH010000000
VERSION     DBNGOH010000578.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2333)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2333)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2333
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1369550"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1067
                     /locus_tag="AC159P_11175"
     CDS             <1..1067
                     /locus_tag="AC159P_11175"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF039907.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HIR3696052.1"
                     /translation="DREYVKSLERQTYQPTKGYTEGTAEISPADRARKIADIIAESEK
                     AGVVSAGFHQARGQAGGFATKNGNFGYERTSLVSLSMTARTPDGASSGYFLRNHFDVN
                     RLDTARVAREAIRRALEGRDARAMEPGTYTVILETQAVADLLGGLAFGFNARAAEEGR
                     SPYSLPGGRTRVGEKIFDERLSVYSDPWNVELPGSMAAQEGLPAQKLYIVKDGVLQNL
                     VFSRSWAARKKTEPTPGPVNTILESTAPTATAEEMIRSTERGLLVSRFWYIRSTDPRT
                     ASLTGLTRDGVWYIEGGKIEHPVKNFRFNQSIIRMLAPGNVELVGASERVSSSEGQNA
                     SLLPALKLKAFTFTSQSEAV"
     gene            1151..1426
                     /locus_tag="AC159P_11180"
     CDS             1151..1426
                     /locus_tag="AC159P_11180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696053.1"
                     /translation="MKRVIIVCGVLAIVLLTVSVPACYFGERAAQQELGKLSPAEREL
                     RQFDIEYVRLVLPGIALVLSGLLSGVVAIALWVVELVSARREAGASR"
     gene            complement(1452..2072)
                     /locus_tag="AC159P_11185"
     CDS             complement(1452..2072)
                     /locus_tag="AC159P_11185"
                     /inference="COORDINATES: protein motif:HMM:NF025426.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4252 domain-containing protein"
                     /protein_id="HIR3696054.1"
                     /translation="MNIPVRAILKIVVFFSFLAFFASAVAAQPASAEKGRIRLDSIER
                     LSGKARETVNVNVDGDLIKFAVVLLSDDDPEEKAIKEIALDLRGVHVRRLEFKAGGQY
                     AEGDLADIRAQLRAPGWSKLVDVKGGGAQDWEIEDAEIYVATQDGRIEGLVVIVAEPR
                     SLTVANVVGAIDLEKLKRLGGKLGLPKITINRRKITITTEETKKKP"
     gene            complement(2144..>2333)
                     /locus_tag="AC159P_11190"
     CDS             complement(2144..>2333)
                     /locus_tag="AC159P_11190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696055.1"
                     /translation="RAPRRARPREDLLAAVTERQQSAKEQLVYALRLTGAKLGEVKTK
                     VRGAGEAGNASEERNRIR"
BASE COUNT          366 a          828 c          751 g          388 t
ORIGIN      
        1 tcgaccgcga gtacgtcaag tcgctcgaac gtcaaaccta tcagccgacc aaaggctaca
       61 ccgaaggcac cgccgaaatc tcgcccgccg accgcgcgcg caagatcgcc gacatcatcg
      121 ccgagtccga aaaggcgggc gtcgtcagcg cgggcttcca ccaagcgcgc gggcaggccg
      181 gcggcttcgc cacgaagaac ggcaacttcg gctacgagcg cacgagcttg gtgagcctct
      241 cgatgacggc gcgcacgccg gacggcgcga gcagcggcta tttcctgcgc aaccacttcg
      301 acgtgaaccg gctcgacacc gcccgcgtcg cgcgcgaggc catccgccgc gccctcgaag
      361 gccgcgacgc ccgcgcgatg gagccgggca cctacaccgt catcctcgaa acgcaggccg
      421 tcgccgacct tttgggcggc ctcgccttcg gcttcaacgc ccgcgcggcg gaagagggcc
      481 gcagccctta ctcgctgccc ggaggccgga cgcgcgtcgg cgagaagatt ttcgacgagc
      541 ggctgagcgt ctactccgac ccgtggaacg tcgagctgcc cggctcgatg gcggcgcagg
      601 agggcctgcc cgcgcagaag ctctacatcg tcaaggacgg cgtcttgcag aacctcgtct
      661 tctcgcgctc gtgggccgcg cgcaagaaga ccgagccgac gcccggcccc gtcaacacca
      721 tcctcgaatc cacggccccg accgccaccg ccgaagagat gatccgctcg accgagcgcg
      781 gcctgctcgt cagccgcttc tggtacatcc gctcgaccga cccgcgcacg gcttctttga
      841 cgggactcac gcgcgacggc gtctggtaca tcgaaggcgg caaaatcgaa cacccggtca
      901 agaacttccg cttcaaccag agcatcatcc gcatgctcgc gcccggcaac gtcgagctgg
      961 tcggcgcctc cgagcgcgtc agcagcagcg aaggccagaa cgcctcgctc ctccccgcgc
     1021 tcaagctcaa ggccttcacc ttcacctcgc agtccgaggc cgtctgagcc ggaggcgaca
     1081 ccttccgcat cgcggcgcgg aggcggaggc ggaggcggag gcctcgtcga tttatgggag
     1141 gcgaccccgg atgaagagag tcataatcgt gtgcggggtg ctcgcgatag tgctgctcac
     1201 ggtttctgtt cccgcctgtt atttcggcga gcgtgcggcc cagcaggagt tgggcaagct
     1261 gtcgccggcg gaacgggaac tgcggcagtt cgacatcgag tacgtgcggt tggtgctgcc
     1321 cggcatagca ctggttttga gcgggcttct ctccggtgtc gtcgcgattg cgctctgggt
     1381 cgtcgagctg gtgagcgcga gacgggaggc gggcgcaagc aggtgacggc gcccacgcgc
     1441 ggcccgactc ttcacggctt cttcttcgtc tcttcggtcg tgatggtgat cttgcggcgg
     1501 ttgatggtga ttttggggag gccgagcttg ccgccgaggc gcttgagctt ttcgaggtcg
     1561 atggcgccga cgacgttggc gaccgtgagc gagcgcggct cggcgacgat gacgaccagc
     1621 ccttcgatcc tgccgtcctg cgtcgccacg taaatctccg cgtcttcgat ctcccagtcc
     1681 tgtgcgccgc cgcccttcac gtccacgagc ttcgaccagc cgggcgccct gagctgcgcg
     1741 cggatgtcgg cgaggtcgcc ctcggcgtac tgcccgcccg ccttgaactc cagccgccgc
     1801 acgtggacgc cccgcaggtc gagcgctatc tccttgatgg ccttctcttc cgggtcgtcg
     1861 tccgagagca gcacgacggc gaacttgatg aggtcgccgt ccacgttcac gttcaccgtc
     1921 tcgcgcgctt taccggagag ccgttcgatg ctgtcgaggc ggatgcgccc cttctccgcg
     1981 gacgcgggct gcgccgcgac ggcggacgcg aagaaggcga ggaaggaaaa gaagacgacg
     2041 atttttaaga ttgccctgac aggtatgttc atcaccactc ccttcgtccg gcccccgcgg
     2101 cgcgaccgcg tttgaaacgc ggcggcgggc ttcggcgcac gcctcatctg atcctgttcc
     2161 gctcctcgga cgcgttgccg gcctcgcccg cgccgcgcac cttcgtcttc acctcgccga
     2221 gcttggcgcc cgtcaggcgc agcgcgtaga ccaactgctc cttggccgac tgctgccgct
     2281 ccgtgacggc ggccaacaaa tcttcccgcg gcctcgccct gcgcggcgcg cgc
//
LOCUS       DBNGOH010000579         3718 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4667540, whole genome shotgun sequence.
ACCESSION   DBNGOH010000579 DBNGOH010000000
VERSION     DBNGOH010000579.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3718)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3718)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3718
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4667540"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..489
                     /locus_tag="AC159P_11195"
     CDS             <1..489
                     /locus_tag="AC159P_11195"
                     /inference="COORDINATES: protein motif:HMM:NF028235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HIR3696056.1"
                     /translation="LPRLWSLCDIWLSYVQGGVFRGGCFFAAASAEFDSRPGPVRDRI
                     AAIMKEWLDMLHRAVVEAQGAGQLDREVAPAQLAFEFNALEIGANWAFQLHGDRQAFA
                     RAREAILERLRRHSTPGGAALLPPNKARRRRAGGPPNSRPRTAAGVKPRSRTQDAVRS
                     RK"
     gene            486..1355
                     /locus_tag="AC159P_11200"
     CDS             486..1355
                     /locus_tag="AC159P_11200"
                     /inference="COORDINATES: protein motif:HMM:NF014376.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR3696057.1"
                     /translation="MNAAAKVRHTSPVVLERGATWDGIRLTHYRFRAGSLPEHSHPEH
                     LLTVSLGGGCKAEIRTASGFRTRDKVRGSVCVIPAGAPFTTRIEDDSEYLAIYLEPSL
                     VARAASEAGASRGPFEVVESCAASDPVVRSVGLALLAELENENLGGRLYAESLANVLA
                     VHLLRRYATAGVGAAQRFTGGLGGQRLRQVTDFIADNYGGDLSLDDLARVAGMSTFHF
                     AREFKRTTGTTPHQYLIKFRVERAKALLAEGGMPLVEVGAQAGFSHQSHFTRLFRRLT
                     GTTPQSYRLMFQS"
     gene            1455..1910
                     /locus_tag="AC159P_11205"
     CDS             1455..1910
                     /locus_tag="AC159P_11205"
                     /inference="COORDINATES: protein motif:HMM:NF027286.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4760 domain-containing protein"
                     /protein_id="HIR3696058.1"
                     /translation="MHMETKPQDALVILKLYEIRGEALMRAARAWFFAEFAPRSGRGI
                     VKLMLSGERQSAYYRMVASHWEVAAALVNHGGVDEKLFLEANSEHVVVFAKLQPFLAE
                     IRETMGEPDYLANLERLVMKVPDIEKKLENRRRLLASWAQAGATAAGES"
     gene            2084..3646
                     /locus_tag="AC159P_11210"
     CDS             2084..3646
                     /locus_tag="AC159P_11210"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HIR3696059.1"
                     /translation="MSVASTAAAPSSAARRRSGVLVVAACFAAAKLLVHFLTNGNYGY
                     FRDELYFLACGEHLDWGYVDHAPLVGAAAWFARRFVGESLFALRLLPAVAGALKVLLT
                     GLLVREFGGGKFAAALACLCVLVAPIYLGIDTLFSMNAFEPVFWMTCALAITLAVGRG
                     EPRWWLLFGVAAGLGLQNKHSMLFFGFAVLVGLLLTPARRALASKWFWMGGVLALVIF
                     LPNLIWEYRHDWATLELLSNVGKTGKNVLVSPFEFMAQQVLMLHPLTLPVWAAGLWFL
                     LFDREGRRLRPLGIAYLVLLALMIALKGKNYYTQAVYPLLFAAGGVMFERVVARRRAR
                     WLAFAYPLVLLAGGAALAPLVLPVLTVEKFLRYQDALGVKPPKTEVGHEGPLPQHFGD
                     MFGWPETVAEVARIYHSLPPAEREKAAVYAGNYGEAGAVDFYGPRHGLPKAISPHQNY
                     FLWGPRHYTGEVVILLQSEREEAERACASVEEAGRVGHPYAMAEEHYTIYICRGLRQP
                     LAELWPSLKHWN"
BASE COUNT          567 a         1226 c         1331 g          594 t
ORIGIN      
        1 ctgccgcgcc tgtggtctct gtgcgacatc tggctctcct acgtgcaggg cggggtgttc
       61 cgcggcgggt gttttttcgc cgccgcctcc gccgagttcg acagccgccc cggccctgtg
      121 cgcgaccgca tcgccgcgat catgaaggaa tggctcgaca tgctgcaccg cgccgtggtc
      181 gaggcgcagg gcgcggggca gctcgaccga gaggtcgcgc ccgcgcagct cgccttcgaa
      241 ttcaacgcgc tggagatcgg cgccaactgg gccttccaac tgcacggcga ccggcaggcc
      301 ttcgcgcgcg cgcgcgaagc catcctcgaa cgcctgcgtc gccactcgac gccgggcggc
      361 gccgcactgc tcccgcccaa caaggcgaga cgccgccgcg cgggcggccc gccgaactcc
      421 cggccacgaa ccgcagccgg agtgaagcct cgctcaagaa ctcaagacgc agtgaggtcg
      481 cggaaatgaa cgcagcagca aaggtgcggc acacgtcgcc cgtcgtcctc gaacgcgggg
      541 ccacgtggga cggcatccgt ctcacgcact accgcttccg cgccgggagc ttgccggagc
      601 acagccaccc cgaacacctc ttgacggtct cgctcggcgg cggctgcaag gccgagatac
      661 ggacggcgag cggcttccgc acgcgcgaca aggtacgcgg cagcgtgtgc gtcatccccg
      721 cgggcgcgcc cttcacgacg cgcatcgagg acgactcgga atacctcgcc atctaccttg
      781 agccgtcgct cgtcgcgcgc gccgcttcgg aggcgggcgc ttcgcgcggc cccttcgagg
      841 tcgtcgagag ttgtgcggcg agcgaccccg tggtgcgcag cgtcgggctg gcgctgctcg
      901 ccgagctgga gaacgaaaac ctgggcgggc ggctctacgc ggagtcgctg gcgaacgttc
      961 tcgcggtgca tctgctgcgg cgctacgcga cggcgggcgt cggcgcggcg cagcgcttca
     1021 cggggggtct gggcggtcaa aggctgcggc aggtgacgga cttcatcgcg gacaactacg
     1081 gcggcgacct cagcctcgac gacctggcgc gcgtggccgg gatgagcacc ttccacttcg
     1141 cgcgcgagtt caagcggacg accggcacga cgccgcacca gtacctcatc aagttcaggg
     1201 tcgagcgcgc caaggcgctg ctggccgagg gcgggatgcc gctcgtcgaa gtcggcgcgc
     1261 aggcaggctt cagccaccag agccacttca cgcgactctt ccgcaggctg acggggacga
     1321 cgccgcagtc ctaccgcctg atgttccagt cgtagccgcc ggagtagcaa gaatcgtcaa
     1381 ccccgagcaa taacgggaaa gacgcgcggc gcacgccgcc cgtaacctcg ccgcatcact
     1441 cgaagggagg acggatgcac atggagacga agccgcagga cgcgctcgtc atactgaagc
     1501 tctacgagat caggggcgag gcgctgatgc gcgcggcgcg cgcgtggttc ttcgcggagt
     1561 tcgcgccgcg cagcggcagg gggatcgtga agctgatgct gagcggcgag cggcagagcg
     1621 cctattaccg catggtcgct tcgcactggg aggtcgccgc cgcgctcgtc aaccacggcg
     1681 gcgtggacga gaagctgttc ctcgaagcca attcggaaca cgtcgtcgtc ttcgccaagc
     1741 tccagccctt cctcgccgag atcagggaga cgatgggcga gccggactac ctcgcgaacc
     1801 tcgaacggct cgtgatgaag gtgccggaca tcgagaagaa gctggagaac cgtcggcgcc
     1861 tgctcgcgag ctgggcgcag gccggcgcga cggcagcggg cgagagttaa aagaagtatc
     1921 gaaagcggga ggggccgccg gagcgtggat gacaggcgtg ctccgacggc ccctccgtct
     1981 tcggcgtcgg cggcgcgggt cgtgctcagt cgaagcggag cgaaacacgg cccgcgcggc
     2041 gcgcgttata taatccgtga ctcccgctct cgaaaccgaa accatgtccg tagcctcgac
     2101 cgcagccgct ccgtcttccg ccgcgcgccg ccgaagcggg gtgctcgtcg tcgcggcgtg
     2161 cttcgcggcg gcgaagctgc tcgtccactt tctgacgaac ggcaattacg ggtacttccg
     2221 cgacgagctg tacttcctcg cgtgcggcga gcacttggac tggggctacg tggatcacgc
     2281 gccgttggtg ggggcggcgg cgtggttcgc gcgcaggttc gtgggagagt cgctgttcgc
     2341 gctcaggttg ttgcctgcgg tggcgggcgc gttgaaggtg ttgctgacgg ggctgttggt
     2401 gcgagagttc ggcggcggca aattcgccgc ggcgctcgcg tgtttgtgcg tgctggtcgc
     2461 gccgatctat ctgggcatcg acacgttgtt ctcgatgaac gcgttcgagc cggtcttctg
     2521 gatgacgtgc gcgttggcga tcacgctcgc ggtcgggcgc ggcgagccgc gttggtggct
     2581 gctgttcggc gtcgcggcgg gactcggcct acagaacaag cactcgatgc tcttcttcgg
     2641 cttcgcggtg ctcgtcggcc tcttgctgac gcccgcgcgc cgcgcgctcg cgtcgaagtg
     2701 gttctggatg ggaggcgtgc tggccctcgt catcttcctg ccgaatctga tctgggagta
     2761 caggcacgac tgggcgacgc tcgaactcct gagcaacgtc gggaagacag gcaagaacgt
     2821 cttggtgtcg ccgttcgagt tcatggcgca gcaggtgttg atgctgcacc ctttgacgct
     2881 gccggtgtgg gcggcgggcc tctggttcct gctcttcgac cgcgagggca ggcgcctgcg
     2941 cccgctcggc atcgcctacc tggtgttgct cgcgctgatg atcgcgctca aggggaagaa
     3001 ctattacacg caggccgtct accctctgct cttcgcggcg ggcggcgtca tgttcgagcg
     3061 ggtggtcgcg cggcggcggg cgcgctggct cgcgttcgcg tacccgctcg tgctcttggc
     3121 gggcggggcg gcgctggcgc cgttggtgct gccggtgctg acggtcgaga agttcctgcg
     3181 ctatcaggac gcgctcggcg tcaagccgcc gaagacggag gtcgggcacg aggggcctct
     3241 gccgcagcac ttcggggaca tgttcggctg gcccgagacg gtcgcggagg tggcgcgcat
     3301 ctaccactcg ctgccgcccg ccgagcgcga gaaggccgcc gtctacgcgg gcaactacgg
     3361 cgaggcgggc gcggtcgatt tctacgggcc gcgccacggc ctgccgaagg ccatcagccc
     3421 gcaccagaac tactttctct gggggccgcg ccactacacg ggcgaggtcg tcatcctctt
     3481 gcagtcggag cgcgaggagg ccgagcgcgc ctgcgcgagc gtcgaggagg ccgggcgcgt
     3541 cggccacccc tacgcgatgg ccgaagagca ctacaccatc tacatctgcc gcggcctgcg
     3601 gcagccgctc gccgaactct ggccgagcct gaagcactgg aactgaaaag cagtaggcag
     3661 caggcagcgg gcagcaggca gtcaagcgag aggcttgggg cgtcgagtct caagcctc
//
LOCUS       DBNGOH010000580         4290 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_5035002, whole genome shotgun sequence.
ACCESSION   DBNGOH010000580 DBNGOH010000000
VERSION     DBNGOH010000580.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4290)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4290)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4290
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_5035002"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..168)
                     /locus_tag="AC159P_11215"
     CDS             complement(<1..168)
                     /locus_tag="AC159P_11215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002770300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-methyltransferase"
                     /protein_id="HIR3696060.1"
                     /translation="MNKKILVAERRNYLVTTVAQAKDIALAWLTEIELNQVIDFGLPE
                     VDDRYHIWRVPL"
     gene            complement(271..954)
                     /locus_tag="AC159P_11220"
     CDS             complement(271..954)
                     /locus_tag="AC159P_11220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017654065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HIR3696061.1"
                     /translation="MENNILARLPVEALERLRPHFEQMSLAHGFRVIVPDEPIRHVYF
                     PLTCLLSLVTRMEDGSAVESGTIGREGMSGIPVILKARTTTMETLVQVPGEAIRVKAE
                     VVREEYERGDVLHDLLNRYLHIIVVNGSQSAACNALHRVEARFCRWLLLSSDGIGSDE
                     VALTQEYLAVMLGVRRATVTEVAIKIQEAGLIRYHRGQIKILDRAGVERLACECYART
                     RAEYQRLFS"
     gene            1293..1688
                     /locus_tag="AC159P_11225"
     CDS             1293..1688
                     /locus_tag="AC159P_11225"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR3696062.1"
                     /translation="MTLPRCRVLCVDDNDDTCFMLRTLLGTSGYEVETADGVNEALRL
                     ARTERFDLYIIDNRYPDGTGADLCRQLREASPDTHVVFYSGAAYGSDREEGLGAGATA
                     YVVKPAIDELLKTIKRLTAAEPCAASNAA"
     gene            complement(1788..2861)
                     /locus_tag="AC159P_11230"
     CDS             complement(1788..2861)
                     /locus_tag="AC159P_11230"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR3696063.1"
                     /translation="MSRKVVLLGPLPPPYGGVSVYLSALREHLAPDGVRTWALFGGEG
                     ERDARTTFVKHRRLGLVPVVLREGRGARVLDATHFHFEYPNKILLPLWLACKRVLGFE
                     WHKHVHDGTLPTRYPGFTRAQRRLFRLAVRHVDEFVVVSEELRRWLLEEVGAEQKITV
                     VPSLLPIPRRHLERALTASIEDRLTPFLRAARRVCSVGVFTESYGFAHAAAAVERLRA
                     ETGEEIGLVLLDGTFARDEGYRDEVLRGREWVTVLENVPSPDVYQIMRRSDAFVRAVS
                     HEGYGISRVEATWCGLPVVATAAGETRGMLIYEFGDVEGLARQLRRALFDSRARDVAG
                     WAALYRGEAEENLRALRRALLTD"
     gene            2933..4012
                     /locus_tag="AC159P_11235"
     CDS             2933..4012
                     /locus_tag="AC159P_11235"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR3696064.1"
                     /translation="MSRQLPSSHRDETTRGSTVAGETPRERVVDDEKTVGRAVADEES
                     RDGAARSVSVVVPSYNHARFVERTLRSVFAQTHAPSELLVIDDGSADDSPRVVERVLR
                     DCPFPCALIARANRGLCATLNEGLSRSRGEFFAYLGSDDLWLADFLGARLALLEARPH
                     AVLAYGHAYLIDERDRVVDCTKDWASYADGDARAMLLGTVAPMSPTVVYRRDALARHG
                     WDEAARLEDYDLYLRLSVDGEFAFDPRVLAAWRRHGANASADQRMMLEEQLAAQRRVL
                     PSFGMTPGEIERLHARISFARAEDFMRVGAKREALGLIRRNLGGVTSARNLARMALRL
                     LVPHRLMLRRARGRAAERYGSMELQ"
BASE COUNT          705 a         1503 c         1393 g          689 t
ORIGIN      
        1 taaagggacg cgccaaatgt ggtaacggtc atcgacttca gggagaccga agtcgatgac
       61 ctgattgagt tcgatttccg ttagccaagc tagcgctatg tccttagcct gggcaacggt
      121 agtcaccaaa taatttcttc tctccgcaac aagtattttt ttattcacga ttggaattgc
      181 ccctctcagc taacttaggg ccggacgaag gatagtaaca cgcaggggcg gcgggcgcat
      241 gaagggcggg ggcgggggat gggcgcgtgg ttagctgaag aggcgttggt actcggctct
      301 ggtgcgggcg tagcattcgc aggccagccg ctcgacgccc gcgcggtcga gaatcttgat
      361 ctggccgcgg tggtagcgga tgaggccggc ttcctgaatc ttgatggcga cctcggtgac
      421 ggtggcgcgg cgcaccccga gcatgacggc gaggtactcc tgcgtgagcg ccacctcgtc
      481 cgagccgatg ccgtcgctgc tcagcagcag ccagcggcag aaccgcgcct cgacgcggtg
      541 cagcgcgttg caggccgccg actgcgagcc gttgacgacg atgatgtgca ggtagcggtt
      601 cagcaggtcg tgcaacacgt cgccccgttc gtactcctcg cggacgactt cggccttcac
      661 gcggatggct tcgccgggca cctgcaccag cgtctccatc gtcgtcgtcc tcgccttgag
      721 gatgacgggg atgcccgaca tgccttcgcg cccgatggtg ccggactcga ccgccgagcc
      781 gtcctccatc cgcgtcacga gcgagagcag gcaggtgagc gggaagtaga cgtggcggat
      841 gggttcgtcc ggcacgatga cgcggaagcc gtgcgcgagc gacatctgct cgaagtgggg
      901 gcgcagccgt tcgagggctt cgaccggcag tcttgcgaga atgttatttt ccatgtcgag
      961 agttcccccg acccgtcccc gagagcggcg gcgcccgcgc cgccgccgcc ggccacggcc
     1021 tcactcacac ttccgctcac gtccgtgggt gaattcagtc gccgaccaga cacacattag
     1081 actcctatcg tacagacatg tacaacccgt tgccgtacaa ttcggcatca attctttggg
     1141 gtggcggggt gtccggacga cactgtaccc acgcctcttt tcaaacttaa caccttacct
     1201 cttgtgaccg agtcggacgt tccaggggag tcttgacctt atcgatgtaa gccagccgcg
     1261 cggctgccaa cgacccgcgc cgggagacgt tcatgacact acctcgctgc cgcgtcctct
     1321 gcgtggacga caacgacgac acctgcttca tgctgcggac gctgctcggc acgtccggct
     1381 acgaggtcga gaccgccgac ggcgtcaacg aagccctgcg cctcgcccgc acggaacgat
     1441 tcgaccttta catcatcgac aaccgctacc ccgacggcac gggcgcagac ctctgccggc
     1501 agctccgcga ggcgagtccc gacacgcacg tcgtcttcta ctcgggcgcg gcctacggga
     1561 gcgaccgcga agagggcctc ggcgcgggcg cgaccgccta cgtcgtcaaa cccgccatcg
     1621 acgagctgct gaagaccatc aagcgtttga ccgccgcgga gccttgcgcc gcttcgaacg
     1681 cggcctgagc agaaccctcc gccacctccc ccctcacgat tccactcgcg ggacgggaga
     1741 cggtcgggcc gcaccggccc cttcacctct cgactcgaca ccgcccgtca atccgtcagc
     1801 aaggctctgc gcagcgcgcg caggttctct tcggcctcgc cgcggtaaag cgcggcccac
     1861 cccgccacgt cgcgcgcgcg cgaatcgaag agcgcgcggc ggagctggcg cgccagccct
     1921 tccacgtcgc cgaactcgta gatgagcatc ccgcgcgtct cgcccgccgc ggtcgccacg
     1981 acgggcaggc cgcaccacgt cgcctcgacg cgcgagatgc cgtagccttc gtggctgacg
     2041 gcgcggacga aggcgtcgct gcgtcgcatg atctggtaca cgtccgggct gggcacgttc
     2101 tccagcaccg tgacccactc gcgcccgcgc aggacttcgt cgcggtagcc ctcgtcgcgc
     2161 gcgaacgtgc cgtcgagcag gacgaggccg atctcctcgc ccgtctccgc gcgcaagcgc
     2221 tcgaccgccg ccgccgcgtg cgcgaagccg taagactcgg tgaagacgcc gaccgagcag
     2281 acgcgccgcg ccgcccgtag gaaaggcgtc agacggtctt cgatcgacgc cgtcagcgcg
     2341 cgttcgaggt ggcgccgcgg tatcgggagc aggcttggga cgaccgtgat cttctgctcg
     2401 gcgccgacct cttcgagcag ccagcgccgc aactcttcgc tgacgacgac gaactcatcg
     2461 acgtgcctga cggccagacg aaagagcctc cgctgcgcgc gcgtgaagcc cggatagcgc
     2521 gtgggcagcg tgccgtcgtg cacgtgcttg tgccactcga agccgagcac gcgcttgcag
     2581 gcgagccaca gcggcagcag aatcttgttc gggtactcga agtggaaatg cgtcgcatcc
     2641 agcacgcgcg ctccgcgccc ttcgcgcagc acgacgggca ccagcccgag cctccgatgc
     2701 ttgacgaagg tagtccgcgc gtcgcgctcg ccctctccgc cgaagagcgc ccacgtgcgc
     2761 accccgtccg gtgcgaggtg ttcgcgcagc gcgctcaggt agacggagac gccgccgtac
     2821 ggcggcggga gcgggccgag gagtacgact tttctgctca tgcggggctg ctatcatgct
     2881 atagactgcg gacgcagttc aaacagccga ccgccgacgc gagttccgat acatgagccg
     2941 tcaacttccc tcttcgcatc gagacgagac gactcgcggg agcaccgttg ccggggagac
     3001 gccgcgcgaa cgagtcgttg acgacgagaa gacggtcggg cgcgccgtcg cggatgagga
     3061 gtcgcgtgac ggggcggcgc gaagcgtctc ggtcgtcgtg ccctcttaca accacgcgcg
     3121 cttcgtcgag cgcacgctgc gctccgtctt cgcgcagacg cacgcgccct cggagctgct
     3181 cgtcatcgac gacggctcgg cggacgactc gccccgcgtc gtcgagcgcg tgctgcgcga
     3241 ctgccccttc ccctgcgcgc tgatcgcgcg cgcgaaccgt ggcctgtgcg cgacgctcaa
     3301 cgaggggctg tcgcgcagcc gcggcgagtt cttcgcctac ctcggctccg acgacctgtg
     3361 gctcgcggac ttcctcggcg cgcgcctcgc gctgctcgaa gcgcgcccgc acgccgtgct
     3421 cgcctacggc cacgcctacc tcatcgacga gcgcgaccgg gtcgtcgatt gcacgaagga
     3481 ctgggcctct tacgccgacg gcgacgcgcg cgcgatgctg ctcggcaccg tcgcgccgat
     3541 gagtccgacg gtcgtctacc gccgcgacgc gctcgcacgc cacggctggg acgaggcggc
     3601 gcggctcgaa gactacgacc tctacctgcg cctctctgtc gatggcgagt tcgccttcga
     3661 cccgcgcgtg ctcgcggcgt ggcgccggca cggcgcgaac gccagcgccg accagcggat
     3721 gatgctcgaa gaacaactcg cggcgcagcg ccgtgtgctg ccgtccttcg gcatgactcc
     3781 cggcgagatc gagcggctgc acgcgcgcat cagcttcgca cgcgccgaag acttcatgcg
     3841 cgtcggcgcg aagcgcgagg cgctcggtct catccggcgc aacctcggcg gcgtcacgtc
     3901 ggcgcgcaac ctcgcgcgca tggcgctgcg cctgttggtt ccgcaccgtc tcatgcttcg
     3961 gcgcgcccga ggtcgcgcgg cagagcggta cggctcgatg gaactgcaat agcagtccgg
     4021 agtctggagt ctgaagtcag aaccgtgacg cgccttgagg ggtaaagtca caagcctcct
     4081 cctcgtcctg actccagact ccggactccg gactccggac tgcttcttga cgcttcacgt
     4141 cgggcgtggc tagactggcc cgaaaggttg gatggatttg aacagcagcg acagagcgcg
     4201 cgccctcgaa cagacccaca acactccgca cagtgattcg agcgcagccg gagcacggca
     4261 cgagccgcag gccggtgccg cgcccgttca
//
LOCUS       DBNGOH010000581         3185 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1188110, whole genome shotgun sequence.
ACCESSION   DBNGOH010000581 DBNGOH010000000
VERSION     DBNGOH010000581.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3185)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3185)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3185
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1188110"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            121..1515
                     /gene="argH"
                     /locus_tag="AC159P_11240"
     CDS             121..1515
                     /gene="argH"
                     /locus_tag="AC159P_11240"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018233185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HIR3696065.1"
                     /translation="MGEDKKLWGGRFTGETDSGFARFNRSFGFDRRLFEADVRGSMAH
                     GEALRAAGVLGEEEAARVAEGLKTLLERAAQEGESYFADETAEDVHSFIEARLVELVG
                     DAGRRLHTGRSRNDQVATAMRLWLREEIDRVEETLREAQRALLDLAGAHADAVLPGYT
                     HLQRAQPVAWAHWCLAYFEMFARDRERLKEVRRRVNVLPLGSAALAGTSQAIDREGVA
                     RALGFDGVTRNSLDAVSDRDFCVEFAAAGSLVMAHLSRLAEDLIIYSTTEFGFIEMGD
                     AISTGSSLMPQKKNPDALELVRGKTGRVYGHAFALLTVVKGLPLAYNKDMQEDKEAVF
                     DVADTVRDSLAVTATVLRNIRLREGRARDAASVGYMNATEMADYLARRGVPFREAHEA
                     VGRMVLHALSKGLELHELELEELKEFSPLVGEDVYDALSLEQTLGTKSRTGGTAPARV
                     AEALAAARASLADG"
     gene            complement(1522..2757)
                     /locus_tag="AC159P_11245"
     CDS             complement(1522..2757)
                     /locus_tag="AC159P_11245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014098814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR3696066.1"
                     /translation="MKLASLVIACALVAGCGGGGPGAAGTGEPTPTPPDDRLRIGAVM
                     SLTGDTARYGISAMQGMQMAVEDANGRGGVRGKRVELIVQDTRSDAGETEQVVRRLVT
                     ERRVHALLGEIVSSRALAVAAVAQEARVPVLTPSSTSPDVTARGDYVFRSCYTDPFQG
                     AAIARFAAGTLGARRAAMLLDAGESYSAQLAEFIRDDFARRGGQIVVTENYAKGDSYF
                     TEQLTRIREAAPEVVFLPGYYREAGLIAREAKKLGITAPLVGGDGWDSRDLYEIGETA
                     LLGSFFSSHYSADDPDPLVQQFVADYVRLYGSLPDAFAATAYDAARIMLDAFERAEGS
                     TDRARIRDALAATRDFPGVTGRITFNAARDAVKPIVIIKIEEGGRYAVQERITPDSLA
                     LPTPTPSPTPQKRRRRRER"
     gene            complement(2783..>3185)
                     /locus_tag="AC159P_11250"
     CDS             complement(2783..>3185)
                     /locus_tag="AC159P_11250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010944002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3696067.1"
                     /translation="YLKKNKGEIAEDLERGFQMFPRLKEREAQKAGTLSGGEQQMLAM
                     ARALMSRPRLLLLDEPSLGLAPLVVHTIFEAIDEIRGKGTTILLVEQNAHAALKHSDR
                     AYVLETGKIVMEGPSSELAADPRIKEAYLGE"
BASE COUNT          472 a          993 c         1207 g          513 t
ORIGIN      
        1 tcttctctct ttttccatcg cctttgttat gcaccgtact tgcataactt gtgcatagat
       61 gcataatcta acacgaaaga tgcatattgg caaagtcttg gacgtggcgg actgaaagat
      121 atgggcgaag acaaaaaact gtggggcggg cgcttcaccg gcgagacgga cagtgggttc
      181 gcgcggttca accgctcgtt cggcttcgac cgcaggctgt tcgaggcaga cgtgcgcggg
      241 agcatggcgc acggcgaggc tttgcgcgcg gcgggcgtgc tcggcgaaga ggaggccgcc
      301 cgcgtggccg aagggttgaa gacgttgctt gagagggcgg cgcaggaggg cgagtcgtat
      361 ttcgcggacg agacggcgga ggacgtgcac tcgttcatcg aggcgcggct ggtcgaactc
      421 gtcggcgacg cgggccggcg tctgcacacg ggccggagcc gcaacgatca ggtggcgacg
      481 gcgatgcgtt tgtggctgcg cgaggagatc gaccgcgtcg aagagacctt gcgcgaggcg
      541 cagcgcgcgc tgctcgatct ggccggggcg cacgcggacg cggtgctccc cggctacacg
      601 cacttgcagc gcgcgcagcc cgtcgcgtgg gcgcactggt gcctcgcata cttcgagatg
      661 ttcgcgcgcg accgcgagcg cctgaaggag gtgaggcggc gcgtgaacgt gttgccgctc
      721 ggctcggcgg cgctcgccgg gacgagccaa gcgattgacc gcgaaggggt cgcgcgcgcg
      781 ctcggcttcg acggcgtgac gcgcaacagc ctcgacgccg tgagcgaccg cgacttctgc
      841 gtcgagttcg ccgccgccgg ctcgctcgtc atggcgcacc tgtcgcggct cgccgaagac
      901 ctgattatct actcgacgac ggaattcggc ttcatcgaga tgggcgacgc catctcgacg
      961 gggtcgagcc tgatgccgca gaagaagaac ccggacgcgc tcgaactcgt gcgcgggaag
     1021 acgggccgcg tctacggaca cgccttcgcg ctgctgaccg tcgtcaaggg gttgccgctc
     1081 gcctacaaca aggacatgca ggaggacaag gaggcggtct tcgacgtggc cgacacggtg
     1141 cgcgactcgc tcgcggtgac ggcgacggtg ctgcgcaaca tacggctgcg cgaggggcgc
     1201 gcgcgcgacg cggcgtcggt cggctacatg aacgcgacgg agatggccga ctacctcgcg
     1261 cggcgcggcg tgcccttccg cgaggcgcac gaggcggtcg ggcgcatggt gctccacgcg
     1321 ctctcgaagg ggttggagct gcacgaactc gaactcgaag agctgaagga gttctcgccg
     1381 ctcgtcggcg aagacgtgta cgacgcgctc tcgctcgaac agacgctcgg gacgaaatca
     1441 cggacgggcg gcaccgcgcc cgcgcgcgtc gcggaggcgc tggccgcggc gcgcgcttcg
     1501 ttggccgacg gttgatgaga gctatcgttc ccgccgtctc cgcctcttct gcggtgtcgg
     1561 gctgggcgtg ggggtcggga gcgcgaggct gtcgggggtg atgcgttcct ggacggcgta
     1621 gcgcccgccc tcttcgattt tgatgatgac gatgggcttg acggcgtcgc gggcggcgtt
     1681 gaaggtgatg cgcccggtga cgccggggaa atcgcgcgtg gcggcgaggg cgtcgcggat
     1741 gcgggcgcgg tcggtcgagc cttcggcgcg ctcgaaggcg tcgagcatga tgcgcgcggc
     1801 gtcgtaggcg gtggcggcga aggcgtcggg caggctgccg tagagccgga cgtagtcggc
     1861 gacgaactgc tggacgagcg ggtcggggtc gtccgccgag tagtggctgg agaagaagga
     1921 gccgagcagg gcggtctcgc cgatttcgta gaggtcgcgc gagtcccagc cgtcgccgcc
     1981 gacgagcggc gcggtgatgc cgagcttctt cgcctcgcgc gcgatgagtc ccgcctcgcg
     2041 gtagtagccg ggcaggaaga ccacttcggg cgcggcctcc ctgatcctcg tgagctgttc
     2101 ggtgaagtag ctgtcgccct tcgcgtagtt ctccgtaacg acgatctggc cgccccgccg
     2161 cgcgaagtca tcgcggatga actcggcgag ctgcgcggag tagctttcgc ccgcgtcgag
     2221 cagcatcgcg gcacggcgcg cgccgagcgt gccggcggcg aagcgcgcga tggccgcgcc
     2281 ctgaaagggg tccgtgtagc aactgcggaa gacgtagtcg ccgcgcgcgg tgacatcggg
     2341 gctggtcgag gagggcgtga gcacggggac gcgcgcctcc tgcgcgacgg cggcgacggc
     2401 gagagcgcgc gagctgacga tctcgccgag gagcgcgtgc acgcgccgct ccgtgacgag
     2461 gcggcggacg acctgttcgg tctcgcccgc gtcggagcgc gtgtcctgca cgatcagctc
     2521 gacgcgcttg ccgcgcaccc cgccgcgccc gttcgcgtcc tcgacggcca tctgcatccc
     2581 ttgcatcgcg gagatgccgt agcgcgccgt gtcgcccgtc agagacatga cggcgccgat
     2641 cctgagtctg tcgtcgggcg gcgtcggcgt cggctcgccc gtgcccgccg cgcccggccc
     2701 gccgccgccg caaccggcga cgagggcgca cgcgatgacg agcgacgcga gtttcatcaa
     2761 cgtctgtacg ggacggggaa gttcactcgc cgaggtaggc ttccttgatg cgcgggtcgg
     2821 cggcgagttc actggagggg ccttccatga cgatcttgcc ggtttcgagg acgtaggcgc
     2881 ggtcggagtg tttgagcgcg gcgtgcgcgt tctgctcgac gaggaggatg gtcgtgcctt
     2941 taccgcgaat ctcgtcgatg gcctcgaaga tggtgtggac gacgagcggg gccagtccga
     3001 gcgaaggctc gtcgaggagc agcaggcgcg ggcgcgacat cagcgcgcgc gccatcgcca
     3061 gcatctgctg ctcgccgccc gagagcgtgc ccgccttctg cgcctcgcgt tccttgaggc
     3121 gcgggaacat ctggaagccg cgctcgaggt cttcggcgat ctcgcccttg ttcttcttga
     3181 ggtag
//
LOCUS       DBNGOH010000582         3130 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_5035724, whole genome shotgun sequence.
ACCESSION   DBNGOH010000582 DBNGOH010000000
VERSION     DBNGOH010000582.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3130)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3130)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3130
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_5035724"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..474)
                     /locus_tag="AC159P_11255"
     CDS             complement(<1..474)
                     /locus_tag="AC159P_11255"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR3696068.1"
                     /translation="MAKRKKGDALMDETADVVVIGGGVVGASVAYHLTGGSCRDVLVL
                     ERGAREGAGSTAKATGGVRAQFATEINVRMSLYSIEFFKRFAEATGHDCGYEPNGYLF
                     FATSEEQLSVLRANRERQSGFGLKNVELVGAREIAEMVPPLRTDDVAGGVFCPTDG"
     gene            670..2955
                     /locus_tag="AC159P_11260"
     CDS             670..2955
                     /locus_tag="AC159P_11260"
                     /inference="COORDINATES: protein motif:HMM:NF025869.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA-like domain-containing protein"
                     /protein_id="HIR3696069.1"
                     /translation="MSTGPLVPPRLDIAHVLFMDIVSYSRLPMDRQTRVLQQLYKVVR
                     GTEEFRRAEAAQQLIRLPTGDGMALVFFGDPESALRCALEVSRALLDMPSLPLRMGVH
                     SGAVYRMDDINTSANVAGGGINTAQRVMDCGDAGHILVSKAVAEMLEQLSTWNGYLHD
                     LGEAEVKHGVRVHIYNVHAHGLGNPALPEKLRKSVAPASAGGYADASWPIHTTQEPRP
                     APPPYQPPQTETTSEQPPTYQQQPPSHQTQQPPQPQPPPPTTAMTEPLQSVTPAGEKR
                     LRVALLYKRNAKPDEQVLKFLEDELTRRGHSVFIDRHLTIGVEWAKEIERQVRTADAV
                     VPLLSPLSVVSEMLTYELQIAHEASQGAEGKPRILPVRINFEDALPDPLGGILAPIQY
                     FLWRGPDQDRQLLAQLLGSLEGPKPAEVEPSEIKQPGGAVALDSKLYIVRPTDDEFLE
                     AIRRRDSIVLVKGARQMGKTSLLARGMQQARAAGSEVVLTDFQKLNASHLDSIDAFFV
                     ALAEMLYDQLGLDCDPEDFWNAKRGASINFERYMRREVLKQIEKPLVWAMDEVDRLLT
                     CPFGSEVFGLFRSWHNERQLDPSSPWERLTLAIVYATEPHLFITDPNQSPFNVGTKLE
                     LRDFTPEQAAELNARLGSPLRDPAEVARMYALLGGHPYLVHRGLYEMKQRGLSIDDFE
                     ATADRDEGPYGDHLRRILVLLARDPELTEIVRGVLNGQPCPTMDSFYRLRSAGLLTGD
                     TAREVSMRCRLYENYLKRHLL"
BASE COUNT          550 a         1189 c          916 g          475 t
ORIGIN      
        1 cccgtcggtc gggcagaaga cgccgcccgc cacgtcgtcc gtgcgcaacg gcggaaccat
       61 ctcggcgatc tcccgcgcgc cgaccagctc gacgttcttc agcccgaagc ccgactgccg
      121 ctcgcggttc gccctgagca cggacagctg ctcctcgctc gtcgcgaaga agaggtagcc
      181 gttcggctcg tacccgcagt cgtgcccggt cgcctcggcg aagcgcttga aaaactcgat
      241 ggagtagagc gacatgcgga cgttgatctc ggtggcgaac tgcgcgcgca cgccgcccgt
      301 cgccttggcc gtagaacccg cgccctcgcg cgcgccgcgc tccagaacca gcacgtcgcg
      361 gcagctccct cccgtcaggt ggtaggccac gctcgcgccg accacgccgc cgccgatcac
      421 caccacgtcc gccgtctcgt ccatcaaagc gtcgcctttc tttctctttg ccacgtccgg
      481 cgccggtgtt gtgttaaaat ccgccgtgtc cgaccgcgcg gcgtccgtcc agcaaggaat
      541 cgatgccgac ccaaattatc agcgccacca cacattttga aagaccaacc tcgacccttc
      601 catcaaacga cccacgcggc agggcttcgg tttcaagaac aaaggtatcg ttcggagttt
      661 tagaaagaca tgtcaacagg ccccttagtt cccccccggc tcgacatcgc gcacgtcctc
      721 ttcatggaca tcgtcagcta ctcgcgcctc ccgatggaca ggcagacgcg cgtcctgcaa
      781 cagctctaca aggtggtgcg cggcaccgaa gagttccgcc gcgccgaggc tgcgcagcag
      841 ctcatccggc tcccgaccgg cgacggcatg gcgctcgtct tcttcggcga cccggagtcg
      901 gcgctccgct gcgcgctcga agtcagccgc gctcttttag acatgccctc gctccctctg
      961 cggatgggcg tccacagcgg cgccgtctac cgcatggacg acatcaacac cagcgccaac
     1021 gtcgccggcg gcggcatcaa cacggcgcag cgcgtgatgg actgcggcga cgccggccac
     1081 atcctcgtct cgaaggccgt cgccgagatg ctcgaacagt tgagcacctg gaacggctac
     1141 ctccacgacc tcggcgaggc cgaagtcaag cacggggtgc gcgtccacat ctacaacgtc
     1201 cacgcccacg gactcggcaa ccccgcgctg cccgagaaac tgcgcaagtc ggtcgcgccc
     1261 gcttcggcgg gcggatacgc ggacgcgagc tggcccatcc acaccacgca ggagccgcgc
     1321 cccgcgcctc ccccctatca accgccgcag accgagacga cctccgagca gccgcccact
     1381 tatcaacagc agccgccttc gcatcagacg cagcagcccc ctcaacctca accgccgccg
     1441 cccaccacag cgatgacaga gccgcttcag tccgtcacgc ccgcgggcga gaagcggctc
     1501 cgcgtggcgc tgctctacaa gcggaacgcg aagccggacg agcaggtctt gaagtttctc
     1561 gaagacgagt tgacgcggcg cggccacagc gtcttcatcg accgccacct gaccatcggc
     1621 gtcgagtggg cgaaggagat cgagcggcag gtgcgcacgg cggacgcagt ggtgccgctg
     1681 ctctcgcccc tctcggtcgt gagcgagatg ctgacctacg aattgcagat cgcgcacgag
     1741 gcgtcgcagg gggccgaggg caagccgcgc atcctgcccg tccgcatcaa cttcgaggac
     1801 gcgctgcccg accccctggg cggcatcctc gcgccgatcc agtacttcct ctggcgcggc
     1861 cccgaccaag accggcaact gctcgcgcaa ctgctcggct cgctcgaagg gccgaagccc
     1921 gccgaggtcg agccttccga gatcaagcag ccgggcggcg ccgtcgcgct cgactcgaag
     1981 ctctacatcg tgcggcccac cgacgacgag ttcctggagg ccatccggcg ccgcgacagc
     2041 atcgtcctcg tcaagggcgc gcggcagatg ggcaagacct ccctcttggc gcgcggcatg
     2101 cagcaggcgc gcgccgccgg ctccgaggtc gtgctcacgg acttccagaa gctgaacgcc
     2161 tcgcatctcg actccatcga cgccttcttc gtcgcgctcg cagagatgct ctacgaccag
     2221 ctcggcctcg actgcgaccc cgaggacttc tggaacgcga agcgcggcgc gagcatcaac
     2281 ttcgagcgct acatgcggcg cgaggtcttg aagcagatcg agaagccgct cgtctgggcg
     2341 atggacgaag ttgatcgcct gctgacctgc ccgttcggca gcgaggtctt cggcctcttc
     2401 cgctcgtggc acaacgagcg gcagctcgac ccctcttcgc cgtgggagcg cctgacgctc
     2461 gccatcgtct acgccaccga gccgcacctc ttcatcaccg acccgaacca gtcgcccttc
     2521 aacgtcggca ccaagctcga actgcgcgac ttcaccccgg agcaggccgc cgaactcaac
     2581 gcccgcctcg gctcgccgct ccgcgacccg gcggaggtcg cgcgcatgta cgcgcttctc
     2641 ggcggccacc cctacctcgt ccaccgcggc ctctacgaga tgaagcagcg cggcctctcc
     2701 atcgacgact tcgaggccac cgccgaccgc gacgaggggc catacggcga ccacctccgc
     2761 cgcatcctcg tcctcctcgc gcgcgacccc gaactcaccg agatcgtccg cggcgtcctc
     2821 aacggccagc cctgcccgac gatggacagc ttctaccggc tccgcagcgc cggcctcctc
     2881 accggcgaca cggcgcgcga ggtctcgatg cgctgccgcc tctacgaaaa ctacctcaag
     2941 cgtcacctgc tctgaagagg ggccggaggt cagcggtcgg gggccggtaa agatcatcac
     3001 cgtcagtcgc gtgaactact gcgcgttgca tagaactatt gcccgttgca tagtgccact
     3061 gctcgttgca tgaaccttca agatgaacct gccgacttcg attaaagtcc gaaacccggg
     3121 gccccgcccc
//
LOCUS       DBNGOH010000583         2130 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2470793, whole genome shotgun sequence.
ACCESSION   DBNGOH010000583 DBNGOH010000000
VERSION     DBNGOH010000583.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2130)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2130)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2130
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2470793"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            557..1765
                     /locus_tag="AC159P_11265"
     CDS             557..1765
                     /locus_tag="AC159P_11265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR3696070.1"
                     /translation="MIAEMEKPTTTPNTVILDPDQKSPRAQEFEEKLGTRIVGQERSV
                     RRMSGLYQIFLAGMNPPNRPVGTMLFLGPTGSGKTRVVEAAAEVLFGDPNAVIKIDCA
                     EFQHSHEIAKLIGSPPGYLGHRETSPMLTQENLDRYHTEEIKLSLVLFDEIEKASDSL
                     WQLLLGILDKATLTLGDNRRVDFSKTMVVMTSNLGAREMSELISGGMGFAPAKPSHNP
                     NDSEVDQKIYRTAVEAARRKFSPEFMNRIDKVVVFRSLKEHHLRQILDLELQAVQDRI
                     MKSAGTKFVFQCSDAAKDMLLKEGIDFKYGARHLKRAIERFLVYPLSNLVATEQVGLG
                     DLVYIDLNEANGKLTFSKRSGGVLITGVSEYEEEPEHPEAKSGGAAVPLPAQAKVARR
                     GQGRGDKTEN"
     gene            complement(1850..>2130)
                     /locus_tag="AC159P_11270"
     CDS             complement(1850..>2130)
                     /locus_tag="AC159P_11270"
                     /inference="COORDINATES: protein motif:HMM:NF025030.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HIR3696071.1"
                     /translation="KVEEDEFVRRAAAISLGQLASREAVPVLVAAHSNERAPDDIRRE
                     AARALGLIGDPAAIPALRSALTARDPHLANIAFEALRKLDPASATRPT"
BASE COUNT          390 a          708 c          673 g          359 t
ORIGIN      
        1 gcgaagacct catcacgcac ctcggcctcg aatactccga ctgagcgcga ggcccgacca
       61 cttcgacgaa aacgagggcg cgtccgcggt gcggacgcgc ccttcgcttt tccggcggcg
      121 cgccgcaaga cggcgggcac gctttgctac atccgcgcgg gcggcgccga cgtttaagtg
      181 cagcgtcggc gctgcccgcc gcctttccga aaaaatggag ggcgacaatt cttgtcagtg
      241 tttccctgac aacgtgtgtc acccgcgaag ctcgcgggcg ctttggcggc ggaaattatt
      301 tttgtaattc gcacattttg tggtgacaaa taattcttag tcgtatatat agtgctctcg
      361 tttttgttgc gccgtcgccc cgcggcttgt attcagcgaa ccacgaaaaa aaacttaaca
      421 tcaaactgca cagcggttcg ttctttgcat ggagtggatt ccggcggcgc ggtgccgagt
      481 gtgccgcagc cgaccggcgc ggcgccaaaa gcagtagggc tttccgggtc gcacgctccg
      541 gcagaggaga acgagcatga ttgccgagat ggagaaacca actacaacgc cgaacacggt
      601 cattctcgac cccgaccaga agagtccgcg cgcgcaggag ttcgaggaga agctcggcac
      661 gcgcatcgtc ggacaggagc gctccgtgcg ccgcatgtcg ggcctctatc agattttcct
      721 cgccgggatg aatccgccga accgccccgt cggcacgatg ctcttcctcg gccccactgg
      781 ctcgggcaag acgagggtcg tcgaggccgc cgccgaggtg ctcttcggcg acccgaacgc
      841 ggttatcaag atcgactgcg cggagttcca gcactcgcac gagatcgcca agctcatcgg
      901 cagcccgccg ggctacctcg gccaccgcga gacctcgccg atgctgacgc aggagaacct
      961 cgaccgctac cacaccgagg agatcaagct ctcgctcgtc ctcttcgacg agatcgagaa
     1021 ggcttccgac tcgttgtggc agctcctgct cggcatcctc gacaaggcga cgctcaccct
     1081 cggcgacaac cgtcgcgtgg acttctcgaa gacgatggtc gtgatgacct cgaacctcgg
     1141 cgcgcgcgag atgtccgagc tgatctccgg cgggatgggc tttgccccgg cgaagccttc
     1201 gcacaacccg aacgactccg aggtcgatca gaagatttac cgtacggcgg tcgaagcggc
     1261 gcggcgcaag ttctcgcccg agttcatgaa ccgcatcgac aaggtcgtcg tcttccgctc
     1321 tctgaaggag caccacctgc ggcagattct cgacctcgaa ttgcaggccg tgcaggatcg
     1381 catcatgaag agcgccggga cgaagttcgt cttccagtgc tcggacgcgg cgaaggacat
     1441 gctcctcaag gaaggcatcg acttcaagta cggcgcgcgc cacctgaagc gggccatcga
     1501 gcgcttcctc gtctacccgc tgtcgaacct ggtggcgacc gagcaggtcg gcctcggcga
     1561 cctcgtctat atcgacctca acgaggcgaa cggcaagctc accttctcga agcgttcggg
     1621 cggcgtcctc atcacgggcg tctccgagta cgaagaggag ccggagcacc ccgaagccaa
     1681 gtcgggtggc gccgccgtgc cgctccccgc gcaggcgaag gtcgcgcgcc gcgggcaggg
     1741 tcgcggcgac aagaccgaga actgaaccct caccaccgac gattcgcaca cgaaagccgg
     1801 gcggagccac actccgcccg gctttttagt cggcactctt gacgtagctc taagtcgggc
     1861 gcgtcgcgct cgcggggtcg agcttgcgca gcgcctcgaa cgcgatgttc gcgaggtgcg
     1921 ggtcgcgcgc cgtcagcgcg gagcggagcg ccgggatcgc cgcggggtct ccgatgaggc
     1981 cgagcgcgcg cgccgcctcg cgcctgatgt cgtcgggcgc gcgctcgttc gagtgcgcgg
     2041 cgacgagcac gggcacggcc tcgcggctcg ccaattgccc gagcgagatg gccgcggcgc
     2101 ggcgaacgaa ctcgtcctcc tcgaccttcc
//
LOCUS       DBNGOH010000584        10211 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2836769, whole genome shotgun sequence.
ACCESSION   DBNGOH010000584 DBNGOH010000000
VERSION     DBNGOH010000584.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 10211)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10211)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10211
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2836769"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..475
                     /locus_tag="AC159P_11275"
     CDS             <1..475
                     /locus_tag="AC159P_11275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696072.1"
                     /translation="IFNNGAVIRASLLITAAQPDISTSANGPLGRAAVLNITNPCIAP
                     PGEPFSVTTTRPVGSGTTGNCSSTETETVPTRLLIMLTGVRSATPATVTVRIKDTDLT
                     GTAIIAVRPSLTPGFDQIEVELPASLAGAGDSPIIVTVGGVSSRPADSAPRITIQ"
     gene            522..1769
                     /locus_tag="AC159P_11280"
     CDS             522..1769
                     /locus_tag="AC159P_11280"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR3696073.1"
                     /translation="MLVTIYGLRTSFMLIGLDAIPLTEPLAGVGHYTSELARALARAS
                     AADEFELAYPSTYPPLDLFVVDKVKPPSNLKAARVGVGPAGRHWWSLGLPRYVSRRGI
                     QLFHGTNYDVPLWRRCATVLTVHDLSLFTRPETHEARRVWRARRRLPLMARAATLVVT
                     PTEAVRGEVCQRLGVRPEKVCAVHEAARGVFRPLAFAATADARRRLGADDNFLLAVGT
                     IEPRKNLRALVRAFELVMRETTTITTTATTQATATNEATMKATLDGLRLVIAGGKGWL
                     SDPLFAEVERSPVKDRIVFTGYVTDEDLRALYSSCRLFAYPSLYEGFGLPVLEAMQCG
                     APVLASRIPALSEVAGDAALLVNPDSPEEIARGIVESLRSEAARRRLSDAGLERAAEF
                     SWERAARQTLEVYAEASRIFRFR"
     gene            complement(1772..3685)
                     /locus_tag="AC159P_11285"
     CDS             complement(1772..3685)
                     /locus_tag="AC159P_11285"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HIR3696074.1"
                     /translation="MADIDHEQERRRRGWLALAAVLVFGCAVYAAGVPAKPPGFHIDE
                     SSVAFNAHTIARTGRDEHGARLPLVFRAFGEYKNPVYIYLLAAVFKLTGPSITAARLL
                     SAALGVAAALALGLLARRVTGRREALPLAALAALMTPWLFELSRLVFEVALFPLALAL
                     FLLWLHRAATRARWTWLDAAGIAALLALVTYTYSIGRLLGPLLALGLALFMTRERLAK
                     ILWAWALYALALAPAFAFHYRHPGALTGRFRIVSYFEHQPTWGAAAREFLKRYAGNLD
                     PWRMLMLGDPDPNQLTQIEGCGLLLALTGALAALGLWRFVKEKRRDRWWLYVVYGLLV
                     APVPASLTRELFHTLRLSPLFVFVTALSLLGLEWFLNAAARRRKTGLVAGCIAALALA
                     QGCIFLWRFHTEATTERRAHLLDVEYASRIFEPALAAPTRPVHLADALNNPGYIQAYW
                     HATLRGVPLSEFRRLADDELPPGGGVTITTEEECVRCRVVSESEPYTLYVAASPPRPR
                     GPLPDSAMRAELSVPQPPSLLRAGAKETVTVRVRNAGDGVWLARERAARYQVMLGNHW
                     LGTDGAVLRNDDGRAPLVRDLAPGEETEMPLVVNAPTRAGDYVLEIDALQEGVSWFGL
                     RGSKTARLRVRVE"
     gene            complement(3706..5520)
                     /locus_tag="AC159P_11290"
     CDS             complement(3706..5520)
                     /locus_tag="AC159P_11290"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014425.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArnT family glycosyltransferase"
                     /protein_id="HIR3696075.1"
                     /translation="MDESLKEARWSDGEGWCSVDARRALAAARRFAVARWAEAACALL
                     LLLMSVSLLAATARKTITIDEVVLIPAAYYHFVAGDFHLVHEHPPLCKIVAGLPLLFV
                     QPNEIPRESVDKTTDAAARKWIYQDHFWTSNRAAFGRVAYWPRAAMVVLTVALGVLVF
                     AFARELFGPRAAVLAVLLYTLEPTVLAHGRVVQTDVPAAFGYLAVAYALYRYLGAPSW
                     RRAACLGAACGAALLSKFSMLIIAPLLLAVCAVLLWRAARPGGGEGWAFIPHAAAAAL
                     VTLLVIQAAYYFHSRALDGDDYRWIGAAFPSYAVVVARTVRALSYVLPTDFVLGVFWQ
                     LWHGGEGHAAGLLGMYRQKGWWYYFPVAFALKTTLPFLLLSVAALAWGGSQLIKKRDA
                     RFLILLVPFAVYTLFVMLSPINIGVRYYLPAYTFLFIAGGALLDALLRRGGGRARLAG
                     AAVAVVLVGWMAVEALRAYPDYMAYMNQLASRRERWWYLSDSNVEWGDDVKELAVYLR
                     TRGETRVRAAVLGGFLTLQFHGVEQVDLLTPAAGELPRTRYTAIGASFLNGSTVPVRE
                     VNGVPLSEEQRVNYFDSYRRRAPEKIFGGSIYLYREYE"
     gene            5508..8636
                     /locus_tag="AC159P_11295"
     CDS             5508..8636
                     /locus_tag="AC159P_11295"
                     /inference="COORDINATES: protein motif:HMM:NF021126.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HIR3696076.1"
                     /translation="MIRPSSLPLLRPTPDRLAALAVTLAPLAYFHAAVRGLIYLAPED
                     GMTFNVPLRVAAAAMMREGFLPLWNPYIFAGMPLHASAQAGVLFPLNWFYLFFAPHTA
                     TNLMVVSGYMVAALGAYLYARRAGSCIMGAVVTSLVWQWSGFLVGQLSHVNIVQTAAL
                     LPWLLWAIDGYGAGGGRKYGLLAALFVCLQACAGHQQTLAYSLLLAGAYAVCMAFASR
                     RRDARTRYLSSLVLLAAGVALAAVQILPTLELLRNSVRSEATYEFFSSFSLPPRLLWT
                     FVAPYVYGGGDKRLFLATYVGPPFYGEFIGYVGALTLALAALALILKRDARTKFWTVA
                     ALVCLALALGGHWPYGLYTVVYHVPVLNLFRVPARHLLEVDLALAFLAGRGVTHVMSG
                     ACERARLVRWSLVLGGAVFLLACLAVTVGRPAELKLGRTAPVSIMRAPELFLPVVVAA
                     LSAWALYVFARARGRGASTPLLLAVLALDLCLWGQSSGWRTHSPPRASELWNVPETVK
                     SLRAVGTKEPHRILTYTQPFDPRAPYRKATPEEETEFFLSLQPDTYMPHRFENAAGYD
                     GFGLARYSRLADDMKVWGDLVNPERSIHGAGREFDLLNVRYLVAKRPLRATTTTVSSS
                     AEADMARVSTATERVGGELFAAEDLNTPYLERGARLFFDVPRAEADRFVLLTNLSWSV
                     GVADNAPVGKVRLRAADGRVFDFDLRAGEHTSEWAYDRADIRARIRHRRAPVGTSYTV
                     ADAQQGDYEAHTYVAALTLPARAVITSGEIAVATRAEAPDLSLKVLRASFVDSRDGHA
                     LALRGDWLTKDAPPAVAATPTANVRDGEARKSKAQTPAPVRDAAAERAPAQEERGAAT
                     RWRSVAQLERFGIYENTRALPRAWLADGAAVMTEQAALDTIRTGRLPGGGAWEPRRTA
                     LLEAAASGSGADGAGTQDEGRAEVTRHEPNRVDVKTASASPSILVLGDNHYPGWRARV
                     DGREAELLRVNYNLRGVALPPGDHEVSFVYRPTSLLIGLAVSLVTLAVLLLWMSRSKS
                     RSRMVETLSTEL"
     gene            complement(8856..9806)
                     /locus_tag="AC159P_11300"
     CDS             complement(8856..9806)
                     /locus_tag="AC159P_11300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696077.1"
                     /translation="MRKFIVAALVVACAAAGALGQSQSAPTLRIIAEDGNRLPAELMY
                     GNTKVKPLRLRPGTNTPITIRDTDFFVQQQYVDFLGRFPDQGGFAFWQSNITQCGNNQ
                     GCIDAQRVNVSAAFFLSIEFKESGFLVYRTHRAAFGNLPGKPVPVLRENFIPESRSIA
                     ADVIVGTDGWPERLRNNKTAYMLAFVQRSDFRALYPSSLTPEQFVDGLNAKAGNVLDD
                     AERAALIGELTANNTDAGRASVLLKVAEDGTLHQRELNSAFVFMQYVGYLKRNPNEGP
                     DNSFDGFNFWLDKLNSFNGDFVRAEMVRAFLDSIEYNSRF"
BASE COUNT         1639 a         3656 c         3404 g         1512 t
ORIGIN      
        1 cattttcaac aacggcgcgg tcatccgcgc gtcccttctc atcaccgcgg cgcagcccga
       61 catctccact tcggccaacg ggccgctcgg gcgcgccgcc gtgctcaaca tcacgaaccc
      121 gtgtatcgcg ccgccgggcg aacccttcag cgtgacgacc acgaggcccg tcggctccgg
      181 cacgaccggc aactgctcct cgaccgagac cgagacggtg ccgacgcgcc tgctcatcat
      241 gctcacgggc gtgcgctccg ccacgcccgc gaccgtcacc gtgcgcatca aggatacgga
      301 cctgaccggc acggccatca tcgcggtccg cccgtcgctc acgcccggct tcgaccagat
      361 cgaagtcgag ctgccggcct cgctcgccgg ggccggcgac tcgcccatca tcgtcaccgt
      421 cggcggcgtc agcagccgcc ccgcggactc cgcgccgcgc atcaccattc aataacagcc
      481 gtaaaccgta caccgtaaac cgtgacgagt aagaactgtt cttgcttgtc acgatttacg
      541 gtttacgaac gtctttcatg ctcatcggtc tcgacgccat cccactgacg gagccgctgg
      601 cgggcgtcgg ccactacact tccgagctgg cgcgcgctct ggcgcgcgcc tccgccgcgg
      661 acgagttcga actcgcctac ccttcgacct acccgccgct cgacctgttc gtcgtggata
      721 aagtgaagcc gccctcgaac ctgaaggcgg cgcgcgtggg cgtgggcccc gccgggcgcc
      781 actggtggtc cctggggctg ccgcgctacg tgagccggcg cggcatccaa ctcttccacg
      841 gcaccaacta cgacgtgccg ctgtggcgcc gctgcgccac ggtgctgacc gtccacgacc
      901 tctcgctctt cacccgcccc gagacgcacg aggcccggcg cgtctggcgc gcgcgccgcc
      961 gactgccgct catggcgcgc gcggcgaccc tcgttgtcac gccgaccgag gccgtgcgcg
     1021 gcgaggtctg ccagcgcctc ggcgtgcgcc ccgagaaagt ctgcgccgtc cacgaagccg
     1081 cgcgcggggt cttccgcccg ctcgctttcg cggcgaccgc cgacgcgcgc cgccgcctcg
     1141 gcgcggacga caacttcctg ctcgccgtcg gcaccatcga gccgcgcaag aacctgcgcg
     1201 ccctcgtccg cgcctttgaa ctggtcatgc gcgagacgac gacaataacg acgaccgcaa
     1261 cgacacaagc gaccgcgacg aacgaagcga ccatgaaggc gacgctggac ggacttcgtc
     1321 tcgtcatcgc cgggggcaag ggctggctca gcgacccgct cttcgcggag gtcgaacgct
     1381 cgcccgtcaa agaccgcatc gtcttcaccg gctacgtcac ggacgaagac ctccgcgccc
     1441 tctactcctc gtgccgcctc ttcgcatacc cctcgctcta cgaaggcttc ggcctgcccg
     1501 tcctcgaagc catgcagtgc ggcgcgcccg tcctcgccag ccgcattccc gcgctctccg
     1561 aagtcgcggg cgacgccgcg ctcctcgtca accccgacag ccccgaagag atcgcgcgcg
     1621 gcatcgtcga atcgttaaga agcgaagccg cgcgccgccg actctccgat gccggcctcg
     1681 aacgcgccgc cgaattctcg tgggagcgcg cggcccgaca gacgctcgaa gtctacgccg
     1741 aagcgtcacg catcttccgc ttccgctagt cctactcgac ccgcacgcgc agccgcgccg
     1801 tcttcgaccc gcgcaagccg aaccacgaga caccctcctg caacgcgtcg atctcaagca
     1861 cgtagtcgcc cgcgcgcgtc ggcgcgttga cgacgagcgg catctcggtc tcctcgccgg
     1921 gcgcgaggtc gcgcacgaga ggcgcgcgcc cgtcgtcgtt gcgcagcacc gcgccgtctg
     1981 tgccgagcca gtggttgccg agcatcacct gataccgtgc ggcgcgctcg cgcgcgagcc
     2041 acacgccgtc gcccgcgttg cgcacgcgca ccgtgaccgt ctctttcgcc cccgcgcgca
     2101 gcaacgaagg cggctgcggc acgctcaact cggcgcgcat cgcgctgtcg ggcagcggcc
     2161 cgcgcgggcg cggcggactc gcggcgacgt agagcgtgta cggctcgctc tcggagacga
     2221 cgcggcagcg cacgcactcc tcctcggtcg tgatggtgac gccgccgccg ggcggcagct
     2281 cgtcatccgc gaggcgcctg aactccgaga gtggcacgcc gcgcagcgtc gcgtgccagt
     2341 aggcttgaat gtagccgggg ttgttcaacg cgtcggcgag gtggacgggc cgcgtcggcg
     2401 cggcgagcgc aggctcgaaa atcctgctcg cgtactccac gtccagtagg tgcgcgcgcc
     2461 gctcggtcgt cgcctccgtg tggaacctcc acaggaagat gcaaccttgc gcgagcgcga
     2521 gcgccgcgat gcagcccgcg acgagtcccg ttttgcgacg acgcgccgcg gcgttaagaa
     2581 accattcgag gccgagcagg ctcaaggcgg tgacgaagac gaagagcggc gacaggcgga
     2641 gcgtgtggaa cagctcgcgc gtgagcgagg caggcacggg cgcgaccaag aggccgtaga
     2701 cgacgtagag ccaccagcgg tcgcgccgct tctctttgac gaagcgccag aggccgagcg
     2761 cggcgagcgc gcccgtcagc gcgagcaaga ggccgcagcc ctcgatctgc gtgagctggt
     2821 tcgggtccgg gtcgccgagc atcagcatcc gccacgggtc gaggttgccc gcgtagcgct
     2881 tgaggaactc gcgcgccgcc gccccccatg tcggctgatg ttcgaagtaa gagacgatgc
     2941 ggaacctgcc ggtcagcgcg cccggatgcc ggtagtggaa cgcgaaggcg ggcgcgagcg
     3001 cgagcgcgta cagcgcccac gcccacagga ttttcgccag ccgctcccgc gtcatgaaca
     3061 gcgcgaggcc gagcgcgagg agcggcccca gcagcctccc aatcgagtac gtgtacgtga
     3121 cgagcgcgag cagcgcggct atccctgccg cgtccagcca cgtccagcgc gcgcgcgtgg
     3181 cggcgcggtg cagccacagc aaaaagagcg cgagcgcgag cgggaagagc gcgacctcga
     3241 agacgagccg gctcagctcg aagagccacg gcgtcatgag cgccgcgagc gccgcgagcg
     3301 gcagcgcctc gcgcctgccg gtgacgcgcc gcgcgagcag gcccagcgcg agcgccgccg
     3361 cgacgcccaa cgccgcgctc agcagccgcg ccgccgtgat gctcggcccc gtcagcttga
     3421 agacggcggc gagcagatag atgtagacgg ggttcttgta ctcgccgaag gcgcggaaga
     3481 cgaggggcag gcgcgcgccg tgctcatcgc gccccgtccg cgcgatcgtg tgcgcgttga
     3541 aggcgaccga cgactcgtcg atgtgaaaac cgggcggctt cgcgggcacg cccgccgcgt
     3601 agaccgcgca gccgaagacg agcacggcgg cgagcgcgag ccagccgcgc cgccgccgtt
     3661 cctgttcgtg gtcgatgtcg gccatgcggt cagtcgggaa ggggactact cgtactcacg
     3721 ataaagatag atcgagccgc cgaagatttt ctcgggcgcg cggcggcggt acgagtcgaa
     3781 gtaattgacc cgctgctctt cggagagcgg cacgccgtta acttctcgga cgggcacggt
     3841 cgagccgttg aggaagctcg cgccgatggc cgtgtagcgc gtccgcggca gctcgcccgc
     3901 ggcgggcgtg agcaagtcca cctgctcgac gccgtggaat tggagcgtga ggaagccgcc
     3961 cagcaccgcc gcgcgcacgc gcgtctcgcc gcgcgtgcgc aggtacacgg ccaactcctt
     4021 cacgtcgtcg ccccactcga cgttcgagtc cgagagatac caccagcgct cgcgccgcga
     4081 ggcgagctgg ttcatgtaag ccatgtagtc cgggtacgcg cgcaacgcct cgacggccat
     4141 ccagccgacg agcacgaccg cgaccgccgc gccggcgagc cgcgcccgcc cgccgccccg
     4201 ccgcagcaac gcgtccagca gcgccccgcc cgcgatgaag aggaacgtgt aggcagggag
     4261 gtagtacctg acgccgatgt tgatcggact cagcatcacg aagagggtgt agacggcgaa
     4321 cgggacgagc agaatcaaaa atcgcgcgtc ccgttttttt atcaactgag agccgcccca
     4381 cgcgagcgcg gcgacggaga gcaggaggaa ggggagcgtc gtcttcaaag cgaaggctac
     4441 cgggaagtag taccaccagc ccttctgccg gtacatcccg agcagcccgg cggcgtgccc
     4501 ctcgccgccg tgccagagct gccagaagac gccgagcacg aagtccgtcg gcagcacgta
     4561 ggagagcgcg cgcacggtgc gcgccacgac cacggcatag gatgggaagg ccgcgccgat
     4621 ccagcggtag tcatccccgt cgagcgcgcg gctgtggaag tagtaggccg cctggatgac
     4681 gagcagcgtg accagcgccg ccgccgccgc gtgcgggatg aacgcccacc cctcgccccc
     4741 tccgggtcgc gccgcgcgcc acagcagcac ggcgcagacc gcgaggagca gcggcgcgat
     4801 gatgagcatc gagaacttgc tcaggagcgc cgcgccgcac gccgcgccta agcaagccgc
     4861 gcgccgccac gaaggcgcac cgaggtagcg gtagagcgcg taagcgaccg ccagatagcc
     4921 gaaggccgcg ggcacgtccg tctgcacgac gcgcccgtgc gcgagcacgg tcggctccag
     4981 cgtgtagagg aggacggcga gcaccgcggc gcgcgggccg aaaagctcgc gcgcgaaggc
     5041 gaagacgagc acgccgagcg ccaccgtcaa gacgaccatc gccgcgcgcg gccagtaggc
     5101 gacgcgcccg aaggcggcgc ggttgctcgt ccagaagtga tcctgataaa tccacttgcg
     5161 cgccgccgcg tcggttgtct tatccaccga ctcgcgcgga atctcgttcg gctggacgaa
     5221 gagcagcggc agccccgcga cgattttgca gagcggcggg tgttcgtgga cgaggtggaa
     5281 gtcgcccgcg acgaagtggt agtatgccgc cggaatgagc actacttcgt cgatggtgat
     5341 ggtcttgcgc gccgtcgccg ccagcaggct cacagacatc agcaggagca gcagggcgca
     5401 cgccgcctcg gcccagcgcg cgaccgcgaa gcgacgcgcc gcggcgagcg cgcggcgcgc
     5461 gtccacagag caccaccctt cgccgtccga ccaccgcgcc tctttcagtg attcgtccat
     5521 ctagccttcc actactgcgc ccgacgcccg accggctggc cgcgctcgcg gtcacgctcg
     5581 cgccgctcgc ctacttccac gcggcggtgc gcggcctgat ctacctcgcg cccgaagacg
     5641 gcatgacctt caacgtgccg ctgcgcgtcg ccgccgccgc gatgatgcgc gaagggttcc
     5701 tgccgctgtg gaacccttac atctttgcgg ggatgccgct gcacgcgtcg gcgcaggcgg
     5761 gcgtgctctt cccgctcaac tggttctacc tcttcttcgc gccgcacacg gcgacgaacc
     5821 tgatggtcgt ctccggctac atggtggccg cgctcggcgc ctacctctac gcgcggcgcg
     5881 cggggtcttg catcatgggc gccgtggtga cgagtctcgt ctggcagtgg tcgggcttcc
     5941 tcgtcgggca actgagccac gtcaacatcg tgcagaccgc ggcgctgctg ccgtggctgc
     6001 tgtgggcgat tgacggatac ggcgcgggcg gcggcagaaa gtacggcctg ctggccgcgc
     6061 tcttcgtgtg cctgcaagcc tgcgcggggc accagcagac gctcgcctac tcgctgctgc
     6121 tggcgggcgc gtacgccgtg tgcatggcgt tcgcgtcgcg caggcgcgac gcacgcacac
     6181 gttacctctc gtcgttggtg ctgttggcgg cgggtgtcgc gttggccgcc gtgcaaatct
     6241 taccgacgct cgaattgctg cgcaacagcg tgcgctcgga agcgacttac gagttcttct
     6301 cctcgttctc gctgccgccg cggctgctgt ggacgttcgt cgcgccctac gtctacggcg
     6361 gcggcgacaa gcggctcttc ctcgccacct acgtcggccc gcccttctac ggcgagttca
     6421 tcggctacgt cggcgccctg acgctcgcgc tggccgcgct cgccttgata ctcaaacgcg
     6481 atgcgcggac gaagttctgg acagtcgcgg cgctcgtctg tctcgcgctc gcgctcggcg
     6541 gccactggcc gtacggtctc tacaccgtcg tctaccatgt gcccgtgctc aacctcttcc
     6601 gcgtgcccgc gcgccacctg ctggaggtcg atctcgcgct cgccttcctc gcgggccgcg
     6661 gcgtcacgca cgtcatgtcg ggcgcatgcg aacgcgcgcg cctcgtccgt tggtcgctcg
     6721 tcctcggcgg cgcggtcttc ctgctcgcct gtctcgcggt gacggtgggc cgccccgccg
     6781 agctcaagct cggacgcacg gcgcccgtca gcatcatgcg cgcgccggaa ctgttcctgc
     6841 ccgtcgtcgt cgcggccctg agcgcgtggg cgctctacgt cttcgcgcgc gcgcgcggcc
     6901 gcggggcttc gacgccgctg ctgctcgccg tgctcgcact cgatttgtgc ctgtggggcc
     6961 agtcgagcgg gtggcggacg cacagcccgc cgcgcgcgag cgagctgtgg aacgtgcccg
     7021 agacggtgaa gtccctgcgc gccgtcggaa cgaaagagcc gcaccgcata ctgacctaca
     7081 cgcagccctt cgacccgcgc gcgccctacc gcaaggcgac gcccgaggag gagaccgagt
     7141 tcttcctctc cttgcagccc gacacctaca tgccgcaccg cttcgagaac gccgccggct
     7201 acgacggctt cggtctcgcg cgctacagcc gcctcgccga cgacatgaag gtctggggcg
     7261 acctcgtcaa ccccgagcgc agcatccacg gcgcgggccg cgagttcgac ctgctcaacg
     7321 tccgctacct cgtcgccaaa cgaccgctgc gcgcgacgac cacgaccgta tcgtcatccg
     7381 ccgaagcgga catggcgcgc gtctcgacgg ccaccgagcg cgtcggcggc gagctgttcg
     7441 ccgccgaaga cctgaacacg ccctacctcg aacgcggcgc gcgcctcttc ttcgacgtgc
     7501 cgcgcgcgga ggccgaccgc ttcgtcctgc tcacgaacct ctcgtggtcg gtcggcgtcg
     7561 cggacaacgc gccggtcggc aaagtgcgcc tgcgcgcggc ggacggtcgc gtcttcgact
     7621 tcgacctgcg cgcgggcgag cacacctccg agtgggccta cgaccgcgcc gacatccgcg
     7681 cacggatcag acaccgccgc gcgcccgtcg gcacgagcta cacggtcgcc gacgcccagc
     7741 agggcgacta cgaggcgcac acctacgtcg ccgcgctcac gctccccgcg cgcgccgtta
     7801 tcacctccgg cgagatcgcc gtcgccacgc gcgcagaggc gcccgacctc tccctgaaag
     7861 tactccgcgc ctcgttcgtc gattcgcgcg acgggcacgc gctcgcgctc cgcggcgact
     7921 ggctgacgaa ggacgcgccg cccgccgtcg ccgcgacgcc gacagcgaac gtgcgggacg
     7981 gggaagctcg aaagtcgaag gcgcagaccc ccgcgcccgt acgcgacgcc gcggcggagc
     8041 gcgcgcccgc gcaagaggag cgcggggccg cgacgcgctg gcgcagcgtc gcgcagctcg
     8101 aacgcttcgg catttacgag aacacgcgcg cactgccgcg cgcgtggctc gccgacggcg
     8161 cggcggtcat gacggagcag gcggcgctcg acaccatacg caccggcagg ctgcccggcg
     8221 gcggcgcgtg ggagcctcgg cgcacagcgc tcctcgaagc ggcggcgagc ggcagcggcg
     8281 cggacggcgc gggcacgcaa gacgaggggc gcgccgaggt gacgcgccac gagccgaacc
     8341 gcgttgacgt gaagaccgct tccgccagcc cttcgatcct cgtcctcggc gacaaccact
     8401 accccggctg gcgcgcgcgc gtggacgggc gcgaggccga gctgctgcgc gtcaactaca
     8461 acctgcgcgg cgtcgcgctc ccgcccggcg accacgaagt gagcttcgtc taccgcccga
     8521 cctcactcct catcggcctc gccgtctcgc tcgtcacact ggcagtgctc ttgctctgga
     8581 tgagccgaag taagagccgc tctcgaatgg ttgagacgct ttcgacggaa ctgtaggggc
     8641 agggctggtc cctgcccgcg ctcacgcttg attcgacctt gaatcaaacg gaactcacgg
     8701 gcagggacaa gccctgcccc tacaatcgtc acgcgcgctc tacggcccgc cgaaacttgc
     8761 tcgggcggta acgacagaaa ggcaaagagg aagaggcttg agagtgtatc tctcaagcct
     8821 cttgctttta ctgcctactg cctgctgcct gctgtttaga accgtgagtt gtactcgatg
     8881 gagtcgagga aggccctgac catctcggcg cggacgaagt cgccgttgaa gctgttgagc
     8941 ttgtcgagcc agaagttgaa gccgtcgaag ctattgtccg gcccttcgtt cgggttgcgc
     9001 ttgaggtagc cgacgtactg catgaagacg aaggcgctgt tgagttcgcg ctggtggagc
     9061 gtgccgtcct cggccacctt gagcagcacg gaggcgcggc ccgcgtccgt gttgttagcc
     9121 gtcaactcgc ctatgagcgc ggcgcgctcg gcgtcgtcga gcacgttgcc cgccttcgcg
     9181 ttcaggccgt ccacgaactg ctcgggcgtc aagctcgaag ggtagagggc gcggaagtcc
     9241 gagcgctgga cgaaggccag catgtaggcc gtcttgttgt tccgaagcct ttccggccag
     9301 ccgtccgtgc cgacgatgac atcggcggcg atgctccgcg actcggggat gaagttctcg
     9361 cgcaggacgg gcacgggctt gccgggcagg ttgccgaagg cggccctgtg cgtgcggtag
     9421 acgaggaagc cggactcctt gaactcgatc gagaggaaga aggccgccga gacgttgacg
     9481 cgctgcgcgt cgatgcagcc ctggttgttg ccgcactgcg tgatgttgct ctgccagaag
     9541 gcgaagccgc cctgatcggg gaagcgcccg aggaagtcca cgtactgctg ctggacgaag
     9601 aagtccgtgt ccctgatcgt gatcggcgtg ttggtgccgg ggcgcaggcg caggggcttg
     9661 accttcgtgt tgccgtacat cagctcggcg ggcaggcggt tgccgtcctc ggcgatgatc
     9721 cgcaaggtcg gcgcgctctg actctgcccc agcgcgcccg cggcggcaca ggccacgacg
     9781 agcgctgcaa cgataaactt tcgcatcatg gtgctcccaa aaatcgattc aggcaggaag
     9841 tgtcccggtg tttctgtccc aagcgagatg tcggttgctg ctactggggc ggggccgctc
     9901 ccgcgcgcga ccaaaggcag tttagatacc gccccgccgg tttgtcaact aacagatcgg
     9961 gctttgtaaa ccgagagatt caaaacgctt gaggcttgct ccgggcgtcg gcgcgggccg
    10021 cgccgcgagt ctcttcgtcc gcagactttt gacgccgcgc gggccgcgaa agtttgtctt
    10081 tctgcggaaa aaagtgggga aagcattcgt gcgcgtcacc ccggattgcg gaaataaaag
    10141 aggccgcgga tgaacgcgga tgaaacgcgg atttaaaaac aaacaaacaa tcaattctta
    10201 tccgcgttta a
//
LOCUS       DBNGOH010000585         6936 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3937650, whole genome shotgun sequence.
ACCESSION   DBNGOH010000585 DBNGOH010000000
VERSION     DBNGOH010000585.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6936)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6936)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6936
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3937650"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            223..1224
                     /gene="holA"
                     /locus_tag="AC159P_11305"
     CDS             223..1224
                     /gene="holA"
                     /locus_tag="AC159P_11305"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HIR3696078.1"
                     /translation="MGTISREELWKRIKGGRVEPLYLLFGLEDYLRDAAARRIADAAL
                     EGAPLREFNESSFSLSSADVQQAIAAAEQLPMMASRRVVRVSDFARLREDGEGALARF
                     VARPAETSVVIFVADELDKRRKLSKTLLEACTSVEFAALTDGELAAWAKDRLRQLKAT
                     ADERTLRQLIALAGASVRQLANELEKLATAAGPAGYISMEMVEALVGRSRELSNFELT
                     DHLIARNRKRALETLARLLDDGAEPVMLVGLLASSYHRLALAKELMARGAPEQEVFNV
                     VKMPYHQRQDFLATARRADSAELARRLRRIAAADLALKTSLATPRLQLEMLVCELSA"
     gene            complement(1221..1658)
                     /locus_tag="AC159P_11310"
     CDS             complement(1221..1658)
                     /locus_tag="AC159P_11310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696079.1"
                     /translation="MCAEAEPYRDDEEVLKVVRGFESCECAPDDFNHRSHLVVALCYL
                     LREPEEKALARMRGGLLKFLAHHRIDPATVYHETLTVFWLKRVRAFTERAGHGRTLAA
                     LANELAESCGNSRVVFDYYSKELVGSEEARRALVEPDLKPLDF"
     gene            complement(1748..2017)
                     /gene="rpsT"
                     /locus_tag="AC159P_11315"
     CDS             complement(1748..2017)
                     /gene="rpsT"
                     /locus_tag="AC159P_11315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HIR3696080.1"
                     /translation="MPNHKSAEKRQRQNEKRRVINRNNRGRVRTGIKKLRAALNGGEA
                     GDVQNLLPVTVSLIDKAVQKGVLHRNAAARYKSRLTVRANEAAGE"
     gene            2306..3262
                     /locus_tag="AC159P_11320"
     CDS             2306..3262
                     /locus_tag="AC159P_11320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005512332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HIR3696081.1"
                     /translation="MKKIELPPRGIETLFGVHDQNIKYLESLLDVRVNARGQDLSIDG
                     DPKDVAVAERIVEEFAALFQEGKTFTDRELREAFAQIAEDRAYSLRDYFTKARFNPAG
                     KKQVAPKSANQRRYIEAIQNNDVVFGIGVAGTGKTYLAVAMAVQALMQKQVSRIVLAR
                     PAVEAGERLGFLPGDLQEKVDPYLRPLYDALFDLIDAERVTKLLEKRIIEVAPLAFMR
                     GRTLADAFIILDEAQNTTSEQMKMFLTRIGFGSKAVITGDKTQIDLPTGKRSGLIEAE
                     RVLSGIEGIEFIYFTDKDVVRHRLVQMIVRAYEEHTTRGGQM"
     gene            3422..3850
                     /gene="ybeY"
                     /locus_tag="AC159P_11325"
     CDS             3422..3850
                     /gene="ybeY"
                     /locus_tag="AC159P_11325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HIR3696082.1"
                     /translation="MIEVVNRQRKVALDRERWRAFAARALKAAPSGGAGVTVAFVSDR
                     AMRELNRRWRGKAGTTDVLSFPAGQDEFERAAEGATLGDVVVSVERAARQAAEQGHSF
                     ENEVEQLILHGVLHLCGYDHERDGGEMNRLELRLRRRLGI"
     gene            3942..5246
                     /locus_tag="AC159P_11330"
     CDS             3942..5246
                     /locus_tag="AC159P_11330"
                     /inference="COORDINATES: protein motif:HMM:NF013741.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HIR3696083.1"
                     /translation="MEIELEIAATCVLLVALTFLATVDMAFGQLSDVGLRRLIAEREG
                     RRQGDRTGLFLNVILENRPRFRFTLTAALHVLLVAVAVLVTSVSYNLFTNERRFLLIA
                     FFIGLVLTIIFRQVIPRFFSRSNPEKTLLALLPVYRPLHRVLSIFGAPWQSNADRARL
                     HQTEVGAESIETNIDDTDIQALIDVGAEEGILEEEEGELIQSIIEFGDTSADEVMTPR
                     TDIVALPITATVREARDVMIESKYSRIPVYRDQIDNVEGLIYVRDLLCYWPEGKEDDS
                     IEPLLRPAYFVPATKALDELLEEMQKAHVQLALVIDEYGGLAGLVTVEDILEEIVGEI
                     EDEDTTEEEIVEIFEGDNGYYDLPGSTEVGKVERLFDLEIEDDDFTTIAGLVISESGR
                     VPPAGTHLTFRGLDVEVIEADERRIGRLRLRRAVENGAGDEE"
     gene            5236..5658
                     /locus_tag="AC159P_11335"
     CDS             5236..5658
                     /locus_tag="AC159P_11335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696084.1"
                     /translation="MKSRMRRLHVCAGLLLCAFLVGAAGCASSPGRCGERRVESKESP
                     DRKYTVATFVRKCVGARGEETTVTHVNLHGGLGEPPPDAHGLITSGEVFVVEGARKLG
                     LVWKDAKSLSVGCEECAGQKVTKREASWNDVRISFDVK"
     gene            5690..6829
                     /locus_tag="AC159P_11340"
     CDS             5690..6829
                     /locus_tag="AC159P_11340"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homospermidine biosynthesis protein"
                     /protein_id="HIR3696085.1"
                     /translation="MTKKKRSKYLQGRRIDPRPITGDIALADLIDETFMAYNAARLRE
                     ACRLFTRKMLEPDVTVGLSITGALTPAGLGISALIPLMRAGFVDWIITTGANLYHDTH
                     FGIGLALHQGNTSVSDVVLRKEEVVRIYDIFFDYSVLLDTDAFFRRLIEGEEFQRPMS
                     TAEFHNLCGKYVLERERKLGIENKSFLAAAYEFGVPLYTSSPGDSSIGMNVAAKALQG
                     NKLAFDPSLDVNETAAIVLAAKRGAIHGRGDRGKKHGGKSAVFILGGGSPKNFMLQTE
                     PQIQEVLGIDERGHDYFLQVTDARPDTGGLSGATPGEAVSWGKVDPDRLPDAVVCYVD
                     STIALPVVTAYALARHAPRELKRLYDRRGELMELLLSEYEKSERR"
BASE COUNT         1228 a         2213 c         2332 g         1163 t
ORIGIN      
        1 gacgacccgc gctccttctt caacgaggag gaccccgcgg tcgagcgcct ctcgcgcagc
       61 ttcgccgaga gcctcgtctc gaccctcgtc aacggcttcg gcgtcaacga agaaaagccg
      121 tgaaaagccg tgaaccgtaa accgtaaacc gtgataagtg aaaaccgctt tcttcttatc
      181 acggttcacg gtttacgatt cacggtttac ggctcttgaa ttatgggcac gatttcgcgc
      241 gaggagcttt ggaagaggat caagggcggg cgggtggagc ctttgtacct gctgttcggg
      301 ctggaggatt acctgcgcga cgcggcggcg cggcgcatcg cggacgcggc gctcgaaggc
      361 gctcccttgc gcgagttcaa cgagtcgagc ttcagcctgt cgagcgcgga cgtgcagcag
      421 gcgattgccg ccgccgagca gttgccgatg atggcgtcgc gcagggtcgt gcgcgtgtcg
      481 gacttcgcgc ggctgcgcga ggacggcgag ggcgcgctcg cgcgtttcgt cgcgcgcccg
      541 gcggagacct ccgtggtgat cttcgtcgcc gacgagctgg acaagcggcg gaagctcagt
      601 aagacgctgc tcgaagcctg cacgagcgtc gagttcgcgg cgctcacgga cggggagctg
      661 gccgcgtggg cgaaggacag gctccggcag ttgaaggcga ccgccgacga gcggacgctg
      721 cggcagttga tcgcgctcgc gggcgcgagc gtgcggcaac tggcgaacga gctggagaag
      781 ctggcgaccg ccgccggccc cgccggctac atctcgatgg agatggtcga ggcgctcgtc
      841 ggtcgctcgc gcgagctgtc gaacttcgag ctgaccgacc acctcatcgc gcgcaaccgc
      901 aagcgcgcgc tcgaaacgct ggcgcggctg ctcgacgacg gcgccgagcc ggtgatgctc
      961 gtcggcctgc tggccagcag ctaccaccgc ctcgcgctcg ccaaagaact gatggcgcgc
     1021 ggcgcccccg agcaggaggt cttcaacgtc gtcaagatgc cgtaccacca gcgccaggac
     1081 ttcctcgcca ccgcgcgccg cgcagactcg gcggaactcg cgcgccgcct ccgccgcatc
     1141 gccgccgccg acctcgccct caagacctcg ctcgccacgc cgcgcctcca actcgaaatg
     1201 ctcgtctgcg agttgtcggc ttaaaagtcg agcggcttca aatcaggctc gaccaacgcg
     1261 cgccgcgcct cctccgaccc gacgagttct ttgctgtagt aatcgaacac gacgcgcgag
     1321 ttgccgcacg actcggccaa ctcgttcgcc agcgcggcga gcgtgcgccc gtgcccggcg
     1381 cgctccgtga aggcgcggac gcgcttgagc cagaagacgg tcagcgtttc gtggtagacg
     1441 gtggccgggt cgatgcggtg atgcgcgagg aacttcaaca agcctccgcg catccgcgcg
     1501 agcgccttct cttccggctc gcgcagcagg tagcacagcg cgacgacgag gtgcgaacgg
     1561 tggttgaaat cgtccggcgc gcactcgcac gactcgaacc cgcggacgac cttcaacacc
     1621 tcctcgtcgt cgcggtacgg ctcagcctcg gcacacatca cggttctcct ttccgccgtc
     1681 aataaaaaaa cgaggcagac cgaaacgctt cggtctgcct cgcgtggctg tggatgtctg
     1741 gcggctctta ctcgccggcg gcctcgttgg cgcgcacggt gaggcgcgac ttgtagcgcg
     1801 cggcggcgtt gcggtggagc acgcccttct gcaccgcctt gtcgatgagc gagacggtca
     1861 cggggagcag gttttgaacg tccccggcct ctccgccgtt cagcgcggcg cgcagcttct
     1921 tgatgcccgt gcggacgcgc ccgcggttgt tccggttaat gacgcggcgc ttctcgttct
     1981 ggcgctgccg cttttcggct gacttgtgat ttggcatttc cttatccgtg gctccgttaa
     2041 aactcactcg gtacagaacg tcttaggctt acacggtgaa cagtttaccg tccagcgcaa
     2101 agggcagagt atagggacgc cgcgcgcccg tgtcaaggcg cggcggcgcg gggcgcggcc
     2161 cttccccatc cgctttcatt gacacccgcg cggcctgtgt tgtagcttcc aaccacacca
     2221 tgaagaacgg cgaacccttt caagccctca tgcccctgcc cccgcggcgc caaccgtccc
     2281 cgcaacaagg agtgagcaac gccttttgaa gaagatcgag cttccgccac gcggcatcga
     2341 gacactgttc ggagtccacg accagaacat caaatacctg gaatcgctgc tggacgtgcg
     2401 cgtcaacgcg cggggccagg acctctccat cgacggcgac ccgaaggacg tggccgtcgc
     2461 cgagcgcatc gtcgaggagt tcgccgccct cttccaggag ggcaagacct tcaccgaccg
     2521 cgagctgcgc gaagccttcg cacagatcgc cgaagaccgc gcctattctt tgcgcgacta
     2581 cttcaccaag gcgcgcttca accccgccgg caagaaacag gtcgccccca agtccgccaa
     2641 ccagcgccgc tacatcgagg cgattcagaa caacgacgtg gtcttcggca tcggcgtcgc
     2701 cggcaccggc aagacctacc tcgccgtcgc gatggccgtg caggctttga tgcagaagca
     2761 ggtcagccgc atcgtcctcg cacgcccggc ggtcgaggcc ggcgagcgtt tgggctttct
     2821 gcccggcgac ctgcaagaga aggttgaccc ttacctgcgc cccctctacg acgcgctctt
     2881 cgatttgatc gacgccgagc gcgtcaccaa gcttctggag aagcgcatca tcgaagtcgc
     2941 cccgctggcc ttcatgcgcg gcaggaccct tgcagatgcc ttcatcatcc tcgacgaggc
     3001 gcagaacacg acgagcgagc agatgaagat gttcctgacg cgcatcgggt tcggctcgaa
     3061 ggccgtcatc acgggcgaca agacgcagat cgacttgccg acgggcaagc gcagcggcct
     3121 catcgaggcc gagcgcgtgc tgtccggcat cgagggcatc gagttcatct acttcaccga
     3181 caaggacgtg gtgcgccacc gcctcgtgca gatgatcgtc cgcgcctacg aggagcacac
     3241 cacccgcggc gggcagatgt gaaaggccgt gaaccgtcaa ccgtcaaccg cgacaggtga
     3301 aacgcggtga gcggcgaggg gtgttgagag ggatgctgtt ccggctcgtc acccgtcgcc
     3361 cgtcgccgct ctgttttatc ctgtcgcggt cacgtttcac gcttcacggt ttacggcttt
     3421 tatgatcgag gtcgtgaatc ggcagcggaa ggtggcgctc gaccgagagc gttggcgggc
     3481 gttcgcggcg agggcgttga aggcggcgcc gtcgggcggg gcgggcgtga cggtggcgtt
     3541 cgtgtcggac agggcgatgc gggaactcaa tcggcgctgg cgggggaagg cagggacgac
     3601 ggacgtgttg tcgttcccgg cggggcagga cgagttcgag cgggccgccg aaggggcgac
     3661 gctgggggac gtagttgttt cagtggagcg ggcggcgcgg caggccgcgg agcagggcca
     3721 cagtttcgag aacgaggtgg agcagttgat tctgcacggg gtgttgcacc tgtgcgggta
     3781 cgaccacgag cgggacggcg gcgaaatgaa ccggctcgaa ctgcgcctgc ggcgccgact
     3841 tggaatataa tcagcgtaaa ccgtaagccg taaaccgtga cgagtcagag caggtatttc
     3901 atcttgtcac ggttgacggt tgacggtttg acggcttttc tatggagatc gagttagaaa
     3961 tcgcggccac gtgcgtgctg ctcgtcgcgc tgacgttttt ggcgacggtg gacatggcgt
     4021 tcgggcagtt gagcgacgtg gggctgcggc ggctgatcgc ggagcgcgag gggcggcggc
     4081 agggcgaccg gacggggctg ttcctgaacg tcattctgga aaaccgcccg cggttccggt
     4141 tcacgctgac ggcggcgctg cacgtgctgc tcgtcgccgt ggccgtgctg gtcacctcgg
     4201 tctcgtacaa cctgttcacg aacgagcggc ggttcctgct catcgcgttc ttcatcgggc
     4261 tggtgctgac gatcatcttc cggcaggtca tcccgcgctt cttctcgcgg agcaacccgg
     4321 agaagacgct gctcgcgctg ctgcccgtct accgcccgct gcaccgcgtc ctgtccatct
     4381 tcggcgcgcc gtggcagagc aacgccgacc gcgcgcggct gcaccagacc gaggtcggcg
     4441 ccgagagcat cgagacgaac atcgacgaca cggacatcca ggcgctgatc gacgtgggcg
     4501 cggaggaggg catcctcgaa gaagaggagg gcgagctgat ccagtcgatc atcgagttcg
     4561 gcgacacgag cgccgacgag gtgatgacgc cgcgcacgga catcgtcgcg ctcccgatca
     4621 cggccaccgt gcgcgaggcg cgcgacgtga tgatcgagtc gaagtattcg cgcatccccg
     4681 tctatcggga tcaaatcgac aacgtcgagg ggctgatcta cgtgcgcgac ctgctgtgct
     4741 actggccgga ggggaaggag gacgactcca tcgagccgct gctccggccc gcgtacttcg
     4801 tcccggcgac gaaggcgctc gacgagctgc tggaagagat gcagaaggcg cacgtccagc
     4861 tcgcgctcgt gatcgacgag tacggcggcc tcgccggcct cgtcacggtc gaggacatcc
     4921 tcgaagagat cgtcggcgag atcgaggacg aggacacgac cgaggaagag atcgtcgaga
     4981 tcttcgaggg cgacaacggc tactacgacc tgcccggctc gaccgaggtg gggaaggtcg
     5041 agcggctgtt cgacctcgaa atcgaggacg acgacttcac gaccatcgcc ggtctggtca
     5101 tcagcgagtc ggggcgcgtg cccccggcgg ggacgcacct cacctttcgc gggctggacg
     5161 tggaggtgat cgaggccgac gagcggcgca tcggtcggct gcggctgcgg cgcgcggtcg
     5221 agaacggcgc gggggatgaa gagtaggatg cggcggctcc acgtctgcgc cggtctcttg
     5281 ctgtgcgcgt tccttgtcgg ggcggcgggg tgcgcgtctt caccgggccg gtgcggcgag
     5341 cggcgcgtgg agtcgaagga gtcgcctgac cgcaagtaca ccgtggcgac cttcgtgcga
     5401 aagtgcgtcg gcgcacgcgg cgaggagacg accgtcacgc acgtcaacct gcacggcggt
     5461 ctgggcgagc cgccgccgga cgcgcacgga ctcatcacca gcggcgaggt cttcgtcgtc
     5521 gagggcgcgc gcaaactcgg cctcgtctgg aaggacgcga agagtttgtc ggtcggttgc
     5581 gaagagtgcg cggggcagaa ggttaccaag cgagaggctt cctggaacga cgtgcgcatc
     5641 tcgttcgacg tgaagtgagc gattgaatcc gaggacacgc gggagagtga tgacgaagaa
     5701 gaagcggagc aagtacctgc aagggcggcg catcgacccg cgcccgatca cgggcgacat
     5761 cgcgctcgcc gatctgatcg acgagacctt catggcctac aacgcggctc ggctgcgcga
     5821 ggcgtgccgg ctcttcacgc ggaagatgct ggagccggac gtgacggtcg gcctttcgat
     5881 cacgggcgcg ctcacccccg cgggcctcgg catctcggcg ctcatcccgc tcatgcgcgc
     5941 gggcttcgtg gactggatca tcacgacggg cgcgaacctt taccacgaca cgcacttcgg
     6001 catcgggctg gcgctgcatc agggcaacac ctcggtctcc gacgtggtgc tcaggaagga
     6061 ggaggttgtc cgcatctacg acatcttctt cgactactcg gtcttgctcg acaccgacgc
     6121 cttcttccgt cgtctgatag agggcgagga gtttcagcgc ccgatgtcca cggcggagtt
     6181 ccacaacctg tgcggcaagt acgtgctcga gcgcgagcgc aaactcggca tcgaaaacaa
     6241 gtccttcctc gccgccgcgt acgagttcgg cgtgccgctc tacacttcga gtccgggcga
     6301 ttcgtccatc ggcatgaacg tcgcggcgaa ggcgctgcaa gggaacaagc tcgccttcga
     6361 cccctcgctc gacgtgaacg agacggcggc catcgtgctc gccgccaagc gcggcgccat
     6421 ccatgggcgc ggcgaccgcg gcaagaagca cggcggcaag tccgccgtct tcatcctcgg
     6481 cggcggctcg cccaagaact tcatgctcca gaccgagccg cagatacagg aagtcttggg
     6541 catcgacgag cgcggccacg actacttttt gcaagtcacg gacgcgcggc ccgacaccgg
     6601 cggactgtcg ggcgcgacgc cgggcgaggc cgtctcgtgg ggcaaggtcg atcccgaccg
     6661 cctgcccgac gccgtcgtgt gctatgttga ctcgaccatc gcgctcccag tggtcacggc
     6721 ctacgcgctc gcgcgccacg cgccgcgcga gctgaagagg ctctacgacc gccgcggcga
     6781 gttgatggaa ctgctgttat cggagtacga aaaatcggaa aggcgctgaa agcagtaaat
     6841 ggtgaatggt aaatagaaaa agcgtttctg ctatttacca ttcaccattc accatttacc
     6901 aagattgaga tggcgaagaa ggtcattacc gacgac
//
LOCUS       DBNGOH010000586         5540 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4852848, whole genome shotgun sequence.
ACCESSION   DBNGOH010000586 DBNGOH010000000
VERSION     DBNGOH010000586.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5540)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5540)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5540
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4852848"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            45..1511
                     /locus_tag="AC159P_11345"
     CDS             45..1511
                     /locus_tag="AC159P_11345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014101264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HIR3696086.1"
                     /translation="MKRLLSLTTTFALLGAWGTCLAQGRRQESARPPAAAAAAQGETL
                     IRNATILTVTRGTLQNSDLLVRNGKIAAVGQNLRPSANARVIDATGKYVLPGIIDAHS
                     HSMMDGSVNECTNVVTAMTRVSDILNPTDIDLYRELAGGTTTLLLLHGSCNSIGGQSA
                     TVKIKWGHPIESFIFPGAPPGIKFALGENPKRTNFNVGSGVPRRYPATRMGVEYTIRE
                     AFTRARDYKRTWDEYRAASRDNKNLLPPRRDLTLEPLVEILEGKRLVHAHCYRADEIL
                     MLIGLADEFGFKIKTFQHVLEGYKVAREIARHGAGASTFADFWGYKLEAYDAIPHNAA
                     IMTRLGVVVSVNSDSDERARRLNIEAAKMMKYGGLNEEEALKLITLNPAIQLGVQDRV
                     GSIELGKDADLAIWNAHPLSVYARVDQTFVDGELLFDREQDIARRPQLEAERKTLEQA
                     DSNKPPAQGATPARPPTQIRRAYAHDDDLHDDGGDDQR"
     gene            1508..2887
                     /locus_tag="AC159P_11350"
     CDS             1508..2887
                     /locus_tag="AC159P_11350"
                     /inference="COORDINATES: protein motif:HMM:NF014081.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR3696087.1"
                     /translation="MRSTRPVINALPRLAAPITLAAFLLVSSFIVAAAQDIVGPTSGD
                     LGPAGTYVIRNARVVTVSGPDIENGAVVISGGRITAVGQSVNAPAGATEIDARGLTVY
                     PGMIDASTNMALVEIGSGVPGSVDTSELGDMNPNIQAFVALNPHSAHIDVTRFAGVTS
                     AVALPQGGVVSGQAAFLNLNGTTQRELALVPSVALVVNYPTLGPGGGFAALLAAQQGI
                     SQDAVTQRDRRVEELRKLLRDAEAYGRAADAYQRDPRSVPRPETDIRLAALVPYVRGE
                     RPVLFRAERERDIRAAVRFADEMKLKAVIVGGAEAARAASLLKEKNVPVILDGTLNLP
                     LREDDAYDSFYEHAAKVHAAGIRFAISTGDSGAHVRDLPFHAGMSAAFGLPKAEALKA
                     VTLYPAQIMGVADRLGSIEVGKIANLVVADGDLLEARTNVRHLFIGGRQIPLVSRHTM
                     LYDQFKDRK"
     gene            3259..4293
                     /locus_tag="AC159P_11355"
     CDS             3259..4293
                     /locus_tag="AC159P_11355"
                     /inference="COORDINATES: protein motif:HMM:NF012894.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HIR3696088.1"
                     /translation="MRFDTPTALRTAVATFIVVLALAVPATFAQDTTTSGARSTVAAG
                     QKMKVKGVVKSRSGETFVVQDARTGVETTVVLNERTSVKTKGGFFGGGTNFGQTSILR
                     GLNLEVEGRGDGTNLVAEKIRFSDKDLQTARTVEMNVVPVENRVGVAEGRIDQTEQNA
                     QRLSGQIDELAAVSNAARGGAVAAQEQADRAVEGVRVANDRIGTLDEYTPEKSLAVNF
                     KLRSAVLSPEAKTTLDEVATYAKNARGFVIEVTGYAYESRNKNTNRQLSQQRAESVVR
                     YLVENHNIPLRRIITPFGYGNMNPVADNTTREGRAENRRAEIRVLVSRGLSEPGSDMN
                     PSKVSSATPE"
     gene            complement(4500..5264)
                     /locus_tag="AC159P_11360"
     CDS             complement(4500..5264)
                     /locus_tag="AC159P_11360"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018057947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HIR3696089.1"
                     /translation="MRKLKNTSLAAALLLCLTVTASVLAITYGQPDGNRHPNVGALVG
                     TFQSGTFPYCSGTLISPTVFLTAAHCNIGTTVVFVTFDSEFSSHSKLHRGTFYFDPQF
                     NQAQSDTHDIAVVVFDKPVNNITPARLPEAGQLDDLKVNQKFTVVGYGGQEPINQPGG
                     PVIGFLDTREFSVASLNSINPSWLRLSQNQATGDAGACYGDSGGPNVLGAGAGETDIV
                     AATTITGDVFCKATNVVYRTDTPAARNFLSGFVALP"
BASE COUNT         1004 a         1955 c         1751 g          830 t
ORIGIN      
        1 tgtcacggtt cacggttcac ggttcacggc ttttggagat gaacatgaaa agactgctat
       61 cgctcacgac gacgttcgcg ctgctcggcg cgtggggcac ttgtttggcg caggggaggc
      121 ggcaggagag cgcgcgcccg cccgccgcgg cggcggcggc gcagggcgag acgctgatcc
      181 gcaacgcgac gatcctgacg gtgacgcgcg ggacgcttca gaactccgac ctgctcgtgc
      241 gcaacggcaa gatcgccgcc gtcggccaga acttgaggcc gtcggcgaac gcgcgcgtca
      301 tcgacgcgac gggcaagtac gtcctgcccg gcatcatcga cgcgcactcg cactcgatga
      361 tggacggctc ggtcaacgag tgcacgaacg tcgtcaccgc gatgacgcgc gtgagcgaca
      421 tcctcaaccc gaccgacatc gacctctacc gcgagctggc gggcggcacg acgaccctcc
      481 tgctgctcca cggctcgtgc aactccatcg gcgggcagag cgcgaccgtc aagatcaagt
      541 ggggccaccc catcgagagc ttcatctttc ctggcgcgcc gccgggcatc aagttcgcgc
      601 tcggcgagaa cccgaagcgc accaacttca acgtcgggtc gggtgtgccg cgccgctacc
      661 ccgcgacgcg catgggcgtc gagtacacca tccgcgaggc gttcacgcgc gcccgcgact
      721 acaagcgcac gtgggacgag taccgcgccg cctcgcgcga caacaagaac ctcctcccgc
      781 cgcgccgcga cctgacctta gagccgctgg tcgaaatcct cgaaggcaag cggctcgtcc
      841 acgcgcactg ctaccgcgcc gacgaaatcc tcatgctcat cggtctggcc gacgagttcg
      901 gtttcaagat caagaccttc cagcacgtcc tcgaaggcta caaggtcgcc agagagatcg
      961 cgcgccacgg cgcgggcgcc tcgaccttcg ccgacttctg gggctacaag ctcgaagcct
     1021 acgatgccat cccccacaac gccgccatca tgacgcgcct cggcgtcgtc gtctccgtca
     1081 actccgactc ggacgagcgc gcgcgccgcc tcaacatcga ggccgccaag atgatgaagt
     1141 acggcggcct caacgaagaa gaagccctca agctcatcac cctcaacccg gccattcagc
     1201 tcggcgtgca ggatcgcgtc ggctccatcg aactcggcaa ggacgccgac ctcgccatct
     1261 ggaacgcgca cccgctttcg gtctacgcgc gcgtcgatca gaccttcgtt gacggcgagc
     1321 tgctcttcga ccgcgagcag gacatcgccc gccgcccgca gctcgaagcc gaacgcaaaa
     1381 cgctcgaaca ggccgactcc aacaagccgc ccgcgcaagg cgcgaccccc gcccgaccgc
     1441 ccacacagat tcgccgtgcc tacgcgcacg acgacgacct acatgacgac ggaggtgacg
     1501 accagagatg agaagcacgc gacctgtcat caacgccctc ccgcgcctcg cggcccccat
     1561 cacgttagcc gcgttcctgc tcgtctcgtc cttcatcgtg gctgcggcgc aggacatcgt
     1621 cgggccgacg agcggcgacc tcggccccgc gggcacctac gtcatccgca acgcgcgcgt
     1681 cgtcaccgtc agcggccccg acatcgagaa cggcgcggtc gtcatcagcg gcggacgcat
     1741 caccgcggtc ggccagagcg tcaacgcccc cgcgggcgcc accgagatcg acgcgcgcgg
     1801 cctcacggtc taccccggca tgatcgacgc ctcgaccaac atggcgctcg tcgagatcgg
     1861 ctcgggcgtc cccggctccg tggacacttc ggagctgggc gacatgaacc ccaacatcca
     1921 ggcgttcgtc gccctcaacc cgcacagcgc gcacatcgac gtgacgcgct tcgccggggt
     1981 gacgagcgcc gtcgcgctgc cgcagggcgg cgtcgtctcg ggccaggccg cgttcctcaa
     2041 cctgaacggc acgacgcagc gcgagctggc gctcgtgccc tccgtcgcgc tcgtcgtcaa
     2101 ctacccgacc ctcggccccg gcggcggctt cgccgcgctg ctcgccgcgc agcagggcat
     2161 ctcgcaggac gccgtcacgc agcgcgaccg ccgcgtcgaa gagctgcgca agctcctgcg
     2221 cgacgccgaa gcctacgggc gcgcggcgga cgcctaccag cgcgacccgc gctccgtgcc
     2281 gcgccccgag accgacatcc gcctcgccgc gctcgtgccc tacgtgcgcg gcgagcgccc
     2341 cgtcctcttt cgcgccgagc gcgagcgcga catccgcgcc gccgtccgct tcgccgacga
     2401 gatgaaactc aaggccgtca tcgtcggcgg cgccgaagcc gcgcgggccg cctcgctcct
     2461 caaggagaag aacgtccccg tcatcctcga cggcacgctc aacctccccc tgcgcgagga
     2521 cgacgcctac gactccttct acgagcacgc cgcgaaggtg cacgccgcgg gcatccgctt
     2581 cgccatctcc accggcgaca gcggcgcgca cgtccgcgac ctgccgttcc acgccggcat
     2641 gtccgccgcc ttcgggctgc cgaaggcgga agcgctcaag gccgtcaccc tctaccccgc
     2701 gcagatcatg ggcgtcgccg accgcctcgg ctcgattgag gtcggcaaga tcgccaacct
     2761 cgtcgtcgcc gacggcgacc tcctcgaagc ccgcaccaac gtccgccacc tcttcatcgg
     2821 cggtcgccaa atccccctcg tcagccgcca cacgatgctc tacgaccagt tcaaggacag
     2881 gaagtagcag gcggtaggcg gtaggcagta ggcagtaaaa acagaagaag ggcgttgagg
     2941 ctcgggactt cacctcccga gcctttttac tcttcaagcg ataaaggaag aggcttgaga
     3001 cattccatct caagcctctt cctttgactg cctaccgcct actgccttct gccttctgcc
     3061 tactgttccc cgcaatgtgc tcacacaata ttcgcaagct aatcgcaacc acggcggcgc
     3121 acgtttcggc gtaacttatc ctccacaatt aattcactgc cgacgtttgt cttgaagcgc
     3181 acctcgcaag gtcaagcatt gcgcgattgt cccgacggcg cagtggaact ttcggaaatt
     3241 acggaaaggg agaaaactat gagattcgat accccgacag cgctgcggac tgcggtcgca
     3301 acgtttatcg tcgtgctggc gctggccgtg ccggccacgt tcgcacagga caccaccact
     3361 tcgggcgcgc gctccacggt cgccgccggg cagaagatga aggtcaaagg cgtcgtcaag
     3421 agccggagcg gcgagacgtt cgtcgtgcag gacgcgcgca ccggcgtcga gacgaccgtc
     3481 gtcttgaacg agcggacgag cgtcaagacg aagggcggct tcttcggcgg cggcaccaac
     3541 ttcgggcaga cctcgatcct gcgcggcctg aacctcgaag tcgaggggcg cggcgacggc
     3601 accaacctcg tcgcggagaa gatcagattc tccgataaag atttgcagac ggcccgcacg
     3661 gtcgagatga acgtcgtgcc ggtcgagaac cgcgtcggcg tggccgaggg ccgcatcgac
     3721 cagaccgagc agaacgcgca gcgcctctcg ggccagatcg acgagctggc cgccgtctcg
     3781 aacgcggcgc gcggcggcgc cgtcgcggcg caggagcagg ccgaccgcgc ggtcgagggc
     3841 gtccgcgtcg ccaacgaccg catcggcacg ctcgacgagt acacgccgga gaagagcctc
     3901 gccgtcaact tcaagctccg cagcgccgtg ctctcgcccg aggcgaagac gacgctcgac
     3961 gaggtggcga cctacgccaa gaacgcgcgc ggcttcgtca tcgaggtgac gggctacgcc
     4021 tacgagtcgc gcaacaagaa cacgaaccgg cagctcagcc aacagcgcgc cgagtcggtc
     4081 gtgcgctatc tggtcgagaa ccacaacatc ccgctgcggc gcatcatcac gcccttcggc
     4141 tacggcaaca tgaacccggt cgccgacaac accacgcgcg agggccgcgc cgagaaccgc
     4201 cgcgcggaga tccgcgtcct cgtcagccgc ggactctcgg agcccggctc ggacatgaac
     4261 ccgtccaagg tctcctcggc gacgcccgaa tagaccacga cgggcgacca ccgcatcccg
     4321 ccgcgcaagc ggcggaaaca cacgggccgc atcaccctcg cgatgcggcc cgtgtgcgtc
     4381 tatgaagagg ggcgaagagg taaaaagggg aaagggcgaa aagggaaaga agggcgctcg
     4441 ataacacggc ctcttcttcc ttttcgccct ttcccctttt accctcttcc cccttcctgt
     4501 caggggaggg cgacgaagcc gctcaggaag ttgcgcgcgg cgggcgtgtc ggttctgtag
     4561 acgacgttgg tggccttgca gaacacgtcg ccggtgatgg tcgtcgcggc gacgatgtcg
     4621 gtctcgccgg ctccggcgcc gaggacgttg gggccgcccg agtcgccgta gcaggcgccc
     4681 gcgtcgccgg tcgcctgatt ctgcgacaga cgcagccacg acgggttgat cgagttcagc
     4741 gaggccacgg agaactcgcg cgtgtcgagg aagccgatga cggggccgcc cggctggttg
     4801 atcggctcct gcccgccgta gccgacgacg gtgaacttct ggttcacctt caggtcgtcc
     4861 aactggcccg cctcgggcag gcgcgcgggc gtgatgttgt tgacgggttt gtcgaagacg
     4921 acgacggcga tgtcgtgcgt gtcgctctgc gcttggttga actgcgggtc gaagtagaag
     4981 gtgccgcggt gcagcttcga gtgcgagctg aactccgagt cgaaggtgac gaagacgact
     5041 gtcgtcccga tgttgcagtg ggcggcggtc aggaagactg tgggcgagat gagcgtgccg
     5101 gagcagtagg ggaacgtgcc gctttggaac gtgccgacga gcgcgccgac gttcgggtgg
     5161 cggttgccgt cgggctgccc gtaggtgatc gcgagcacgc tcgccgtgac ggtcaggcag
     5221 agaaggagcg cggcggcgag gctcgtgttt ttcaacttgc gcatggcaat tctccagtga
     5281 aaagtttaga gttgaccgag gacacggact tcgcacgaaa tccttaatgg tgcggtgtta
     5341 ctgttgagac gggcaatggc ggcgggtttc cactggccgc cgccgtgtcg gctgtcgggg
     5401 ccgcggattc tatcagagcg cgaccgcgaa gcctaaccgt ccgcgacgcc cccggagcac
     5461 ccctttcgtg aaccttcaga gcgtgccgcc ccggtcactc gaacgactgc tcgacgaact
     5521 cgacgtgttg aagcgccccg
//
LOCUS       DBNGOH010000587         5552 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2653554, whole genome shotgun sequence.
ACCESSION   DBNGOH010000587 DBNGOH010000000
VERSION     DBNGOH010000587.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5552)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5552)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5552
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2653554"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..970)
                     /locus_tag="AC159P_11365"
     CDS             complement(<1..970)
                     /locus_tag="AC159P_11365"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014600.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0071554 - cell wall organization or
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HIR3696090.1"
                     /translation="MSQLDRRDSDFAHLHPTYRAKVTTVLDQLVAEGIPFKLFEGFRS
                     PQRQQYLYGQGRTRPGAKVTNAVAWQSLHQFGVAADLVLHINGQWSWDTSGNKKKCWR
                     RLHEVARDNGLEPLSWELPHLQLSNLKLADLQAGRYPAGGDETWADQLEESIVSWSGN
                     PPSPPPPAEVALRPPMEEIHVAALGLDAPAPGSAGWHNRNSGIEWRYDGRGVYVRGEG
                     SQPLRTGGQPQTCRRIWELYKEPIVSAASKYEVPPEIIMMTIATETAFARNYNFTGPN
                     TFRWEAHVRNTDVNPPSMGDYSAGPMQTLGTTARWVIDAQGLSYQPF"
     gene            complement(1375..3102)
                     /locus_tag="AC159P_11370"
     CDS             complement(1375..3102)
                     /locus_tag="AC159P_11370"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: protein motif:HMM:NF010588.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HIR3696091.1"
                     /translation="MRVTIGQINTTNGDYEGNTEKIIRAIEQARREGSDLIVFPEVCV
                     QGYTSLDWFLDRDIVQSSLKPLEKIIPATRGIAAVVGTVRPTGSLHGRRLFNSAAVIS
                     DGELLGFADKTLLPEYDVFDDPRYFESAKQRRLFDLGREKLGVVVCEDFWNDKTFWRE
                     RLYAHDPTDEVIEMGATVVVSPNASPFNKSKMGQRCAMVSHRSKTARVPVVYVNLVGG
                     NDGILFDGASLVTDCRGETILQAPPFEEFVGTVDLDCGVRDERCLPGDDIETVRRALV
                     LGIRDYASKNRFRRAVLGLSGGIDSAVVAALACEALGAENVLGVMMPSPFSSRGSVED
                     SVAFGNNFGMPVVEHPISAAFEVLTKELELTKPSRGGESLAAENLQSRLRGNILMTIS
                     NAEGRLLLSTGNKSELALGYCTLYGDTNGGLAVVGDVLKTEVFALARQINAGRELIPW
                     AIVNKRPSAELAPDQFDDQSLPPYDALDPILRLYFERKATPEEIIAEGHDAAMTYDIL
                     NRVESPANEFKRRQLPPTLIISRNAIGVGRRRPVTHRYRRQPPAAAEGAVTSAPPDLA
                     DEGRAREAK"
     gene            complement(3140..3349)
                     /locus_tag="AC159P_11375"
     CDS             complement(3140..3349)
                     /locus_tag="AC159P_11375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007304622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HIR3696092.1"
                     /translation="MRYAMFIEKGERNNSAYLPDLPGCLATGKTVEEIKRRMHEAVEL
                     HLRGLREDGLPVSEPTSMAEYVEAA"
     gene            complement(3429..>5552)
                     /locus_tag="AC159P_11380"
     CDS             complement(3429..>5552)
                     /locus_tag="AC159P_11380"
                     /inference="COORDINATES: protein motif:HMM:NF027787.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5050 domain-containing protein"
                     /protein_id="HIR3696093.1"
                     /translation="EPPPLSAYLADAPEALQEVVSDALTKDRDARFQTAKQMLSKLQR
                     LKRRFDAGGSLDHTLTPDSSVASGETSGRTAAASTVGGASLSWGHRTTPLHTTAETMS
                     TVPSVERERAGRKRMLAGVLVVLTLVALAGGAFAVYRFAGSKSSAVGASPFSLSSMKL
                     QKVPASGATISAAISPDAKYAARVVWEAGRSSLRLRQLSTTNERDLVPADGNISYWGQ
                     PAFSRDGGYVYYVAGKKGQPFRELFRVSLLGGEPQKLVFDVDSGVTLSPDGKRLAFRR
                     HMPKTGEDTIVVAGEDGTGEQTVASYKLPERTHYPARSPDGSRIVHYVGGTDKDGYYV
                     NLNAVSVADRKLVPVSTARWRNIGPIAWLPDGSGLVISARDRASLPSTPMQLWFIPYP
                     EGEAQKITNDVNNYVGVSLSADGRTVLAKQGWDVVNVWLAPAGETARVRQATTTGSNG
                     NSGLAWMPDGRLVYDSDASGNIDVWVMNADGTGQKQLTFDQLVDNGAVASPDGRHIIF
                     RSNRGVGWGFWRMNADGSNARELVSNVDGTFNIGGQFSPDSRWFYYVARDAATNKQTV
                     FRVAVEGGEPEKVLDDKVGYVRLSPDGKWLFSTHLEAEFGATAKIYVFPSEGGAATRV
                     IDAPADMLDARDWSPDSKSIDYVATREGVGNLWRLPLAGGKPRQLTDWKSDFIYRFAW
                     SPDGKQLAVSRGTATTDLVLIRDFR"
BASE COUNT          818 a         1829 c         1972 g          933 t
ORIGIN      
        1 cgaagggctg gtacgacaag ccctgcgcgt cgatcaccca gcgggcggtc gtccccagcg
       61 tctgcatcgg cccggcgctg tagtcgccca tgctcggcgg attcacgtcc gtgttccgga
      121 cgtgcgcttc ccagcggaac gtgttcggcc ccgtgaagtt gtaattgcgc gcgaaggccg
      181 tctcggtggc gatggtcatc atgatgatct cgggcgggac ctcgtacttg ctcgccgcgc
      241 tgacaatcgg ctccttgtac agctcccaga tgcggcggca cgtctgcggc tggccgcccg
      301 ttcgcaaagg ctggctgcct tcgccgcgca cgtagacccc gcgcccatcg tagcgccact
      361 cgatgccgga gttcctgttg tgccagcccg cggaacccgg ggcgggcgcg tcgagtccga
      421 gtgcggcgac gtgaatctct tccatcggcg ggcggagggc gacctcggcg ggcggcggcg
      481 gcgagggggg gttgccggac caggagacga tggactcttc gagctggtcg gcccacgtct
      541 cgtcgccgcc cgcggggtat ctgcccgcct gcaagtcggc gagcttgagg ttgctgagtt
      601 gcaggtgtgg cagctcccag ctcaacggct caagcccgtt atcgcgcgcc acctcgtgca
      661 ggcgccgcca gcacttcttc ttgttgccgc tggtgtccca gctccactgc ccgttgatgt
      721 ggagcacgag gtcagccgcc accccgaact ggtgaagcga ctgccaggcg acggcgttcg
      781 tcactttcgc gccgggccgc gtgcggccct gcccgtagag gtactgctgc cgctgaggcg
      841 agcgaaagcc ctcgaagagt ttgaagggta tcccttccgc cacgagctgg tcgagaacgg
      901 tggtgacctt ggcgcggtag gttgggtgca ggtgtgcgaa gtccgagtcg cgtctgtcca
      961 gttgagacat cggagagtga ttcctttcgc gagggtcgat ggggcgcgcg cgcggcgtgt
     1021 cttcgcgccg agtagggttt caggaagtga ggccggcgtg aggcttccca ccgccgataa
     1081 gagcaatgtg cgcgcactat cgcgcgcggc gcgcccctgg aatttcacgg cgcgcgccgc
     1141 acggacatcc tcttcgtggc gcggtggtca aggctgtcaa cgcgtccgca ctccggggaa
     1201 tgggtcgatt tcagacccga catggcccgc cgtgcaagcg caccgtcacg ggccgccgct
     1261 ctaaatcccc ttgaactaca actatgaatg cgggtgggag gcgaaacatt tcatgcgacg
     1321 gcgtgagcac ggatgaggcg ggttagggcc gcggatacga accgacccgc cgcctcactt
     1381 cgcctcgcgc gcgcgcccct cgtcggcgag gtcgggcggg gcggaggtga cggcgccttc
     1441 ggcggcggcg ggcggctggc ggcggtagcg gtgcgtgacg gggcggcggc gaccgacgcc
     1501 gatggcgttg cgcgagatga tgagcgtggg cggcagttgc cggcgcttga actcgttggc
     1561 gggcgactcg acgcggttga ggatgtcgta cgtcatcgcc gcgtcgtgcc cttcggcgat
     1621 gatctcctcg ggcgtggcct tgcgctcgaa gtagaggcgg aggatggggt cgagcgcgtc
     1681 gtagggcggc agggattggt cgtcgaactg gtcgggcgcg agttccgccg aggggcgttt
     1741 gttgacgatg gcccacggga tcagctctcg ccccgcgttg atctggcggg cgagcgcgaa
     1801 gacctcggtc ttgagcacgt cgccgacgac ggcgaggccg ccgttcgtgt cgccgtagag
     1861 cgtgcagtag ccgagcgcca gctccgactt gttgccggtc gagagcagca gccgcccttc
     1921 ggcgttcgag atcgtcatca ggatgttgcc gcgcagccgc gactgcaagt tctccgccgc
     1981 gaggctctcg ccgccgcgcg aaggcttggt cagctccagc tccttcgtca acacctcgaa
     2041 cgccgcggag atggggtgct cgacgacggg catgccgaaa ttattgccga acgcgacgga
     2101 gtcctcgacg ctcccgcgcg aagagaaggg cgacggcatc atcacgccga gcacgttctc
     2161 ggcgcccaaa gcttcgcacg cgagcgcggc caccaccgcc gagtcgatgc cgcccgacag
     2221 gcccaacacc gcgcgccgga aacggttctt gctcgcgtag tcgcggatgc cgaggacgag
     2281 cgcgcgccgc acggtctcga tgtcgtcgcc gggcaggcac cgctcgtcgc gcacgccgca
     2341 gtcgagatcg acggtgccga cgaactcctc gaacggcggc gcctgaagga tggtctcgcc
     2401 gcggcagtcg gtgacgagcg aggcgccgtc gaagaggatg ccgtcgttgc cgccgaccag
     2461 attgacgtag accaccggca cccgcgccgt cttcgagcgg tgcgagacca tcgcgcaccg
     2521 ctgccccatc ttcgacttgt tgaagggcga ggcgttcggc gagaccacga cggtcgcgcc
     2581 catctcgatc acctcgtcgg tcgggtcgtg cgcgtagagc cgctcgcgcc agaacgtctt
     2641 gtcgttccag aagtcctcgc acacgacgac gcccagcttc tcacgcccga ggtcgaacag
     2701 ccgccgctgc ttcgcggact cgaagtagcg cgggtcgtcg aacacgtcgt actcggggag
     2761 caaggtctta tcggcgaagc cgagcagctc gccgtcgctg atgaccgccg ccgagttgaa
     2821 gagcctgcgc ccgtgcagcg agccggtcgg gcgcaccgtg ccgacgaccg ccgcgatgcc
     2881 gcgcgtcgcg gggatgattt tctccagcgg cttcaaagac gactgcacga tgtcgcggtc
     2941 gaggaaccag tcgagcgacg tgtagccctg cacgcacact tccgggaaca caatcaagtc
     3001 cgacccctcg cgcctcgcct gctcgatggc gcggatgatc ttctccgtgt tgccctcgta
     3061 gtcgccgttc gtcgtgttga tctgcccgat ggtgacgcgc atgttgttat tttggatttt
     3121 cgatttagga ttcggtgtgt caggccgcct ccacgtactc ggccatgctt gtcggctcgg
     3181 agacgggcag gccgtcctcg cgcaggccgc gcaggtgcaa ctcgacggcc tcatgcatcc
     3241 gccgcttgat ctcttcgacc gtcttgcccg tggcgaggca gcccggcagg tcgggcaagt
     3301 acgcggaatt gttccgctcg cccttctcga taaacatggc gtagcgcacg ctcggcacct
     3361 ccgcactaca gtccgccctg tgagtaagga taacgaaaaa ggtggtggag cgaaactgcg
     3421 gggcagcgtc acctgaagtc tctgatgagc acgaggtcgg tggtggcggt gccgcgcgag
     3481 acggcgagct gcttgccgtc gggcgaccag gcgaagcggt agatgaagtc gctcttccag
     3541 tcggtgagct gccgcggttt gccgcccgcg agcgggagac gccagaggtt gccgacgcct
     3601 tcgcgggtgg cgacgtagtc gatggatttg ctgtcgggcg accagtcgcg cgcgtcgagc
     3661 atgtccgcgg gcgcgtcgat gacgcgcgtg gccgcgccgc cctccgaggg gaagacgtag
     3721 attttggcgg tcgcgccgaa ctcggcctcc aggtgcgtgg agaagagcca cttgccgtcg
     3781 ggcgagagac gcacgtagcc gaccttgtcg tcgagcacct tctcaggctc gccgccctcg
     3841 accgcgacgc ggaagaccgt ctgcttgttg gtcgccgcgt cgcgcgcgac gtagtagaac
     3901 cagcgcgagt cgggcgagaa ctggccgccg atgttgaacg tgccgtccac gttgctgacc
     3961 agctctcggg cgttgctgcc gtcggcgttc atgcgccaga agccccaccc gacgccgcgg
     4021 ttcgagcgga agatgatgtg acgcccgtcg ggcgaggcga cggcgccgtt atcgacgagc
     4081 tggtcgaagg tcaactgctt ctgcccggtg ccgtccgcgt tcatcaccca cacgtcgatg
     4141 ttgccgctcg cgtccgagtc gtagacgagg cgcccgtccg gcatccacgc gaggccgctg
     4201 ttgccgttcg agccggtggt cgtcgcctgc cgcacgcgcg ccgtctcgcc cgcgggcgcg
     4261 agccagacgt tgaccacgtc ccagccctgc ttggcgagca ccgtgcgccc gtccgccgag
     4321 aggctcacgc cgacgtagtt gttcacgtcg ttcgtgatct tctgcgcctc gccttcgggg
     4381 taggggatga accagagctg catcggcgtg ctcggcagag aggcgcggtc gcgcgcgctg
     4441 atgacgaggc cgctgccgtc gggcagccac gcgatggggc cgatgttgcg ccagcgcgcc
     4501 gtcgagaccg ggacgagctt gcggtcggcg accgagacgg cgttgaggtt gacgtagtag
     4561 ccgtccttgt ccgtgccgcc gacgtagtgg acgatgcggc tgccgtcggg cgacctcgcg
     4621 gggtagtgcg tccgctcggg cagcttgtag gaggcgaccg tctgctcgcc cgtgccgtcc
     4681 tcgcccgcga cgacgatggt gtcctcgccc gtcttcggca tgtggcggcg gaaggcgagg
     4741 cgcttgccgt cgggcgagag cgtgacgccg ctgtccacgt cgaagacgag cttctgcggc
     4801 tcgccgccga gcaaagagac gcggaacagc tcgcggaacg gctgcccctt cttgcccgcg
     4861 acgtagtaga cgtagccgcc gtcgcgcgag aaggcgggtt gcccccagta agagatgttg
     4921 ccgtccgcgg gcacgaggtc gcgctcgttg gtggtcgaga gctgccgcag cctcaaggaa
     4981 gagcggcccg cctcccacac gacgcgcgcg gcgtacttgg cgtcgggcga gatggccgcc
     5041 gagatggtcg cgccgctcgc cggcaccttc tgcaacttca tcgagctgag cgagaacggc
     5101 gacgcgccga ccgcgctact cttcgacccc gcgaagcggt agacggcgaa cgcgccgccc
     5161 gccagagcga ccagcgtgag cacgaccagc acgcccgcca gcatccgctt ccggcccgcg
     5221 cgctcgcgct cgaccgacgg caccgtggac atcgtctcgg cggtcgtgtg cagcggcgtc
     5281 gtgcggtggc cccagctcag cgacgcgccg ccgacggtcg aggcggcggc ggtgcggccc
     5341 gaagtctcgc cgctcgcgac ggaagagtcc ggcgtcagcg tgtggtcgag gctgccgccc
     5401 gcgtcgaagc gccgcttcag acgttgcagc ttcgagagca tctgcttggc ggtctggaag
     5461 cgcgcgtcgc ggtctttggt gagcgcgtcg gagacgacct cctgcaacgc ctcgggcgcg
     5521 tccgcgaggt aggccgagag cggcggcggc tc
//
LOCUS       DBNGOH010000588         5098 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_454977, whole genome shotgun sequence.
ACCESSION   DBNGOH010000588 DBNGOH010000000
VERSION     DBNGOH010000588.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5098)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5098)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5098
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_454977"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(60..941)
                     /locus_tag="AC159P_11385"
     CDS             complement(60..941)
                     /locus_tag="AC159P_11385"
                     /inference="COORDINATES: protein motif:HMM:NF015431.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR3696094.1"
                     /translation="MQIETLKVYCDLVETESFSQAAERNFITQSAVSQQVRGLEEKFE
                     RRLLERVRGRREMRLTQAGEAFYNASREVLRAYGELEESMRTLTGTVSGTVRVATVYS
                     VGLHELPTPVREFMSRYPQAKIDLEYSRTTRVVRDVLDGAVELGVVAFPEKKRGLEVV
                     PLKGDRLVLICPPGHPFARRKKLRAADLKDQDFVLFERDIPTRRAIDRILRAHGVAVR
                     RVAEFDNIETIKRAVEVGLGLAVVPRPSVLDEERSGQLAVVQLAEPEWTRTVGIIYRS
                     DRALGSAARKFIELLTK"
     gene            1049..1675
                     /locus_tag="AC159P_11390"
     CDS             1049..1675
                     /locus_tag="AC159P_11390"
                     /inference="COORDINATES: protein motif:HMM:NF016879.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Coq4 family protein"
                     /protein_id="HIR3696095.1"
                     /translation="MTAYAPDMTLRDARARYYAANGFGADGGDSERWVEVRVGRFPMW
                     FPNTRGRRYAVKFHDLHHVLTGYPTSLEGETEIGAWEVSTGLGRHYVGWLLDLLAFTA
                     GLGVNPRGVHRAFVRGRHSRNLFSREWDERFLSRTVGEVRRSLGLDAKPRRATAGDNV
                     AFVAWSLAGLAVSTAAFPLLVVPLFVVTLPLRLSRRSDRASPVGRRMK"
     gene            1860..2540
                     /locus_tag="AC159P_11395"
     CDS             1860..2540
                     /locus_tag="AC159P_11395"
                     /inference="COORDINATES: protein motif:HMM:TIGR02046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     transmembrane subunit"
                     /protein_id="HIR3696096.1"
                     /translation="MAVRLSSNFVLRKLHQLSGIVPLGAFLLEHLYTNSKAVHPQLGE
                     QHFNDAVKDLQAIPYIIFVEITFIFIPLIYHAVFGLFITWESRPNNLSYPYPRNWFYT
                     IQRVTGIVLFFFILFHVLNFRFGLIPGLNEVSVAHRPDLAYDIVSREFRMPWILAIYF
                     VGIVSTIWHFANGIWLFAVDWGIVIGERAQRYAGYACIFFGVLLLALWINVAAAFIWK
                     GGLLGELL"
     gene            2564..4333
                     /gene="sdhA"
                     /locus_tag="AC159P_11400"
                     /pseudo
     CDS             2564..4333
                     /gene="sdhA"
                     /locus_tag="AC159P_11400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013059399.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
     gene            4558..>5098
                     /gene="sdhB"
                     /locus_tag="AC159P_11405"
     CDS             4558..>5098
                     /gene="sdhB"
                     /locus_tag="AC159P_11405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010303653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HIR3696097.1"
                     /translation="MSTHTNGHGHAPANPPKDVVIRVRRRDAQDKAPYWEEFSIPYRP
                     NLNIISCLMEIRKNPVTKEGKKTTPPVWDMNCLEQVCGICTMVINGKVRQSCSALVDQ
                     LSQPITLEPMSKFPNVRDLVVDRSRMFEHLKQVHAWIEIDGSHDLGPGPKMSPEDVAE
                     RYAYSRCMTCGCCLEACPQY"
BASE COUNT          870 a         1775 c         1592 g          861 t
ORIGIN      
        1 ttgccctgcc cctacaggat tcataacgtt gacgtatgaa cacagccttg agccttgaac
       61 tacttggtta acagctcgat gaacttccgc gccgccgagc cgagcgcgcg gtcgctgcgg
      121 tagatgatgc cgacggtgcg cgtccactcg ggttcggcga gctggacgac ggcgagctgt
      181 ccgctgcgct cctcgtcgag caccgaaggg cgcgggacga cggcgaggcc gaggccgacc
      241 tcgaccgcgc gcttgatcgt ctcgatgttg tcgaactcgg cgacgcggcg caccgcgacg
      301 ccgtgcgcgc gcaggatgcg gtcaatcgcg cggcgcgtcg ggatgtcgcg ctcgaagagg
      361 acgaagtcct gatccttcaa atcggcggcg cgcagttttt tgcgccgcgc gaacgggtga
      421 cccggcgggc agatgagcac gaggcggtcg cccttgagcg gcacgacttc gaggccgcgc
      481 ttcttctccg ggaaggcgac gacgcccagc tcgaccgcgc cgtccagcac gtcgcgcacg
      541 acgcgcgtcg tgcgcgagta ttcgaggtcg atcttggcct gcgggtagcg cgacatgaac
      601 tcgcgcaccg gcgtcggcag ctcgtgcagg ccgacgctgt acacggtcgc cacgcgcacc
      661 gtcccgctca ccgtcccggt cagcgtccgc atgctctctt ccagctcgcc gtaggcgcgc
      721 agcacctcgc ggctcgcgtt gtagaaggcc tcgcccgcct gcgtcaggcg catctcgcgc
      781 cgcccgcgca cgcgctccaa caggcgccgc tcgaacttct cttccagccc gcgcacctgc
      841 tggctcaccg ctgactgcgt gatgaagttg cgctccgccg cctgcgagaa gctctcggtc
      901 tcgacgaggt cgcaatagac tttcagggtt tcgatctgca cggtctttct aattagttcc
      961 tctcagaaat cacgactata tttttcattt taccttataa gcgccgggct ataaaatcgt
     1021 cgtgagataa ttcgggaggg atatgcactt gacggcctat gcgccggaca tgacgctgcg
     1081 cgacgcgcgc gcccgctact acgccgcgaa cggcttcggc gccgacggcg gcgactccga
     1141 gcggtgggtc gaggtgcgcg tcgggcggtt cccgatgtgg tttccgaaca cgcgcgggcg
     1201 gcgctacgcg gtgaagttcc acgacctgca ccacgtcctg accggctacc ccacttcgct
     1261 cgagggcgag acggagatcg gcgcgtggga agtctcgacc ggcctcggtc gccactacgt
     1321 cggctggctg ctcgatctgc tggccttcac cgccggactc ggcgtcaacc cgcgcggcgt
     1381 ccaccgcgcg ttcgtccgcg ggcggcatag ccgcaacctt ttttcgcgcg agtgggacga
     1441 gcgattcctc tcgcgtacgg tcggagaggt gcgccgctcg ctcggcctcg acgcgaagcc
     1501 gcgccgtgcg accgcgggcg acaacgtcgc cttcgtcgcg tggtctctgg ccggcctcgc
     1561 cgtttcgacg gcggcctttc ccctgctggt cgtccccctg ttcgtcgtca cgctgccgct
     1621 ccgtctgtcg aggaggtccg accgcgcctc gcccgtcggg cggcggatga agtagccgct
     1681 tccgtgcgtg cggtcggcgg cggaaaagat tggcgggcga ttgcttttcc gcgagtggtc
     1741 gttataatca tggccgttct aacacacgtc caagccgttc agcgctccac agcgccacac
     1801 ctgcctgcga gggggccgcg gcccgcgccc gcctccggcg aaggagagtc atacccgcga
     1861 tggccgtccg tctgagtagc aatttcgtcc tgcgcaagct ccaccagctc tccggcatcg
     1921 tgccgctcgg cgcgttcctg ctggagcacc tctatacgaa ctcgaaggcc gttcacccgc
     1981 agctcggcga gcagcacttc aacgacgccg tgaaggactt gcaggcgatc ccttacatca
     2041 tcttcgtcga gatcacgttc atcttcatcc cgctcatcta ccacgcggtc ttcgggctgt
     2101 tcatcacgtg ggagtcgagg ccgaacaacc tgagctaccc ctacccgcgc aactggttct
     2161 acaccatcca gcgcgtgacg ggcatcgtcc tcttcttctt catcctcttc cacgtcctca
     2221 acttccgctt cgggctgata ccggggctga acgaagtctc tgtggcgcac cggcccgacc
     2281 tcgcctacga catcgtctcg cgcgagttcc ggatgccgtg gattctggcc atctacttcg
     2341 tcggcatcgt ctcgaccatc tggcacttcg ccaacggcat ctggctcttc gcggtggact
     2401 ggggcatcgt catcggcgag cgcgcgcagc gctacgcggg ctacgcctgc atcttcttcg
     2461 gcgtgctgct cctggcgctc tggatcaacg tcgcggcggc cttcatctgg aagggcggac
     2521 tgctcggaga acttctctaa aacttagggc aacgacgaac ggaatccgca tcgcaatcgt
     2581 cggcggcggc ctcgcgggcc tcgccgccgc catgaagatc gccgaggccg gccacagcgt
     2641 agacctcttc tcggtcgtgc ccgtcaagcg ctcgcactcg gtctgcgcgc agggcggcat
     2701 caacggcgcg gtcaacacga agggcgaggg cgactcgacg tgggagcact tcgacgacac
     2761 ggtctacggc ggcgacttcc tcgccaacca gccgcccgcg aaattgatgt gcgagatggc
     2821 gcccgccatc atttatcttt tcgaccgcat gggcgtcccc ttctcgcgca cgaaggaggg
     2881 actgctcgac ttccgccgct tcggcggcac caagcaccac cgcacggcgt tcgccggggc
     2941 ttccacgggt cagcagctcc tctacgcgct cgacgagcag gtgcgccgct acgaggtgga
     3001 cggcaaggtc aagaagtacg agcactggga gatgctctcg ctcgtgatgg acgaccacca
     3061 ggtctgccgc ggcctcgtgg cgatggagcg ttccagcctt gagctgaaag ccttccccgc
     3121 ggacgccgtc atcatcgcca cgggcgggcc gggcctcatc ttcggcaagt ccaccaactc
     3181 gatggtctgc accggctccg ccgtctccgc ctgttatcag cagggcgcgc gctacgccaa
     3241 cggcgagttc atacaggtgc acccgacctc gatcccgggc gaagacaagc tgcgtctgat
     3301 gtcggagtcg gcgcgcggcg agggcgggcg cgtgtgggtg ccgaaggcgc agggcgacaa
     3361 gcgcgctccc tcttccatcc ccgagtcgga gcgccgctac ttcctcgaag agaagtaccc
     3421 ggcctacggc aacctcgtcc cgcgcgacat cgccacgcgc gaaatcttcc aggtctgtct
     3481 cgaaggctac ggcgtcggcg gcgagaatca ggtctacctc gacctgacgc acatccccgc
     3541 cgagacctta gaccgcaagc tcggcgccat cctcgaaatc tacgagatgt tcgtcggcga
     3601 cgacccgcgc cacgtcccga tgcgcatctt ccccggcgtc cactattcga tgggcggcct
     3661 ctgggtcgat ttcaaccagc ggacgaacat tccgggcctc ttggccgccg gagagtgcga
     3721 ctactcgatt cacggcgcga accgcctcgg cgcgaactct ctggtcagtt gcgtctacgg
     3781 cggcttcgtc gccgcgcccg ccgccgtcga gtacgcgaag aacctcgaac gcaacggcgc
     3841 cgacggctcg cgcgtctacg acgcggagct caagcgccag caggagatca acgacggcct
     3901 cctgaaacag acgggcggcg agaaccagta cgtcatccac gaagagatgg gcaagtggat
     3961 gacggataac gtgacggtcg tccgctacaa cgaccgcctc aaggccaccg acgcgaagct
     4021 gctcgaactc atcgaccgct acaagcgcat ctcgctcaac gactcgaaca tgtgggcgac
     4081 gcacgcgctc ccgcacgcgc gccagctcca gaacatgctc cagctcgcgc gcgtcatcac
     4141 gctcggcgcg ctgaaccgcg acgagtcgcg cggcgcgcac tacaagccgg acttccccga
     4201 ccgcgacgac gagaacttcc tcaagacgac catcgccgag ttctccggcg aaggccccgt
     4261 cttcagttgg gagccggtcg atgtctcgct cgtcaagccg cgcaagcgcg actacagcaa
     4321 gggcaaacag gacacggcga aggctgcggc gggcggcacc gccgaaagct cgcccaccaa
     4381 aggcggcgcc gcgcaggtgc ctgcctccgg ctgggagcag aaaccggaga acctgtccgg
     4441 cccggcggtc tggggccaga acaaagaggc ggtggacggc tccgtctccg gccacgtcga
     4501 gacccgtgag cagcagggca aatccatcga agacgcaggg ggcaaatcgg acgaggtatg
     4561 agcacacaca ccaacggtca cggccacgcg cccgccaacc cgccgaagga tgtcgtcatc
     4621 cgcgtccggc ggcgcgacgc gcaggacaag gcgccctact gggaggagtt ctcgatcccc
     4681 taccgcccga acctgaacat catctcgtgc ctgatggaga tcaggaagaa cccggtcacg
     4741 aaggaaggca agaagacgac gccgcccgtg tgggacatga actgcctcga acaggtgtgc
     4801 ggcatctgca cgatggtcat caacggcaag gtgcggcagt cgtgctcggc gctcgtcgat
     4861 cagctctcgc agccgatcac gctggagccg atgtcgaagt tcccgaacgt ccgcgacctc
     4921 gtggttgacc gctcgcggat gttcgagcac ctcaagcagg ttcacgcgtg gatcgagatc
     4981 gacggctcgc acgacctcgg ccccggcccc aagatgtcgc ccgaggacgt ggccgagcgc
     5041 tacgcctact cgcgctgcat gacctgcggc tgctgcctcg aagcctgccc gcagtacg
//
LOCUS       DBNGOH010000589         3676 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_637370, whole genome shotgun sequence.
ACCESSION   DBNGOH010000589 DBNGOH010000000
VERSION     DBNGOH010000589.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3676)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3676)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3676
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_637370"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1594
                     /locus_tag="AC159P_11410"
     CDS             <1..1594
                     /locus_tag="AC159P_11410"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     NtrB"
                     /protein_id="HIR3696098.1"
                     /translation="VAGATYEGANAALCAAVLVPAQGKSAKGEAGGEQGPTLCVFDRE
                     GSAEETAALIDTARVREWVAARPSSSRRSEDRSQFFLRDGAHKIEYAAPIRFEGRALG
                     VLVAAFDSGRDCGQTEGRLIDAAAQQGALAARISSLYEAARSSSVSLAREVERRTAEA
                     EAQRRFTEAIIDSLPVSLYAVDRSYRVVAWNRNRELGGQGIPRRDVLGRNVFEVLTRQ
                     PRDVMEAEFKRAFETGEIEFIEQETYAADGTTRHWLVSKIPMRDTRGEVSHVITVGED
                     ITSRVEANRAVARSEKLAAVGRLAAGVVHEINNPLATIAACAEALESRTTEGVYGAGA
                     DVDDLGEYLQLIRSEAFRCKQITNGLLDFSRARAGQQAPVQIGEVIESAARLLLHQRR
                     GPSVKINIEVSERLPLVSADEGQLQQAVIILSENAIDAMPEGGTLTLRARRDPSEAGG
                     VLVEVSDTGHGIPPEIRERIFDPFFTTKEVGRGTGLGLAVCYGIVTEHGGRITVDSAP
                     DMGTTFTIHLPATPRPDGEETD"
     gene            1591..3033
                     /locus_tag="AC159P_11415"
     CDS             1591..3033
                     /locus_tag="AC159P_11415"
                     /inference="COORDINATES: protein motif:HMM:NF025885.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HIR3696099.1"
                     /translation="MSENLQPQSAPPTARILVAEDESNLRLVLQKELQRMGHEVRVAP
                     DGEAALRLLEESNVDVLLTDINMPRVDGLELLRRVHQRPNPPEVIMLTGHATVESAIE
                     AMKLGAYDYLSKPYRIAELDALVKQAAEKRRLRVDNQRLRTLVERQTAAPEIVFVSDV
                     MREVMRLVERVAPSDASVLITGESGTGKELIAHAIHKLSRRAEGSFIDLNCAAFQEAL
                     LESELFGYEAGAFSGAKGRKLGLWELADNGTIFLDEITELPSPLQSKLLRAIETNSFY
                     RVGAVRKVQVNVRIVAATNRDVNEVVGDGRFRADLLYRVNGFQIHLPPLRDRAEDIEP
                     ITRHLLRQMGGAHPPEVLPEALAALKAYHWPGNVRQLRNTLERAILLSNDALITAADL
                     PPELSNRAPVYTPAFAPMSPGGPLGSVMNAAGQPAGDVPAPLREVERQQILAALEQTN
                     WHRGKTAEILGISPSTLYRRLRDYNLTRRP"
     gene            complement(3210..>3676)
                     /locus_tag="AC159P_11420"
     CDS             complement(3210..>3676)
                     /locus_tag="AC159P_11420"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3696100.1"
                     /translation="IEAYERALSVKPDDAVVLANLGNSHYAAMNYGAAMKRLKQAARL
                     SPATAVIHNNLGVVQARTGKYGDAFKSFARAGGEYDAHVKLADILETVKRDREAAKHY
                     EAALRLQPGTNALLERLVAIYERTGQPTKADTARRSLGKPRNEQKTTTGGGG"
BASE COUNT          584 a         1269 c         1268 g          555 t
ORIGIN      
        1 cgtcgcgggc gcgacctacg agggcgcgaa cgccgcgctc tgcgccgccg tgctcgtgcc
       61 ggcgcaaggt aagagcgcga agggcgaggc gggcggcgag caggggccga cgctctgcgt
      121 gttcgaccgc gagggcagcg ccgaagagac ggcggcgctg atcgacacgg cgcgcgtgcg
      181 cgagtgggtc gcggcgcgcc cgtcgtcttc gcggcggagc gaagacagga gccagttctt
      241 cctccgcgac ggcgcgcaca agatcgagta cgcggcgccg atccgtttcg agggccgggc
      301 gctgggcgtt ctggtcgcgg ccttcgacag cgggcgcgac tgcgggcaga ccgagggccg
      361 cctcatcgac gccgccgcgc agcagggcgc gctcgcggca cgcatctctt cgctgtacga
      421 ggcggcgcgc agctcctccg tgagcctcgc gcgcgaggtc gagcggcgca ccgccgaggc
      481 cgaggcgcag cgccgcttca ccgaggccat catcgacagc ctgcccgtct cgctctacgc
      541 cgtggatcgc tcgtaccgcg tcgtcgcgtg gaaccgcaac cgcgaactcg gcgggcaggg
      601 catcccgcgc cgcgacgtgc tggggcgcaa cgtcttcgaa gtcctgacgc gccagccgcg
      661 cgacgtgatg gaggccgagt tcaagcgcgc cttcgagacc ggcgagatcg agttcatcga
      721 gcaagagacg tacgcggcgg acggcacgac gcgccactgg ctcgtcagca aaatcccgat
      781 gcgcgacacg cgcggcgagg tctcgcacgt catcacggtc ggcgaagaca tcacttcgcg
      841 cgtggaggcg aacagggccg tcgcgcgctc ggaaaaactc gccgccgttg gaaggctcgc
      901 ggcgggcgtc gtccacgaga tcaacaaccc tctggcgacc atcgcggcct gcgccgaggc
      961 tctggagtcg cgcacgaccg agggcgtcta cggcgcgggc gccgacgtgg atgatctcgg
     1021 cgagtacctc cagctcatcc gcagcgaggc gttccgctgc aagcagatca cgaacggcct
     1081 cctggatttc agccgcgcgc gggcgggaca gcaggcgccc gtgcagatcg gcgaggtgat
     1141 cgagagcgcc gcgcgcctgc tgcttcatca acggcgcggc ccctcggtga agatcaacat
     1201 cgaggtgtcg gagcggctcc cgctcgtctc ggcggacgag ggccagttgc agcaggccgt
     1261 catcatcctc tcggagaacg ccatcgacgc gatgcccgag ggcggcacgc tcacgcttcg
     1321 cgcgcgccgc gacccgtcgg aggcgggcgg cgtcctcgtc gaggtctcgg acacgggcca
     1381 cggcatcccg cccgaaatca gggagcgcat cttcgacccg ttcttcacga ccaaagaggt
     1441 cgggcgcggg acggggcttg gactggccgt ctgttatggt attgttacgg agcacggcgg
     1501 acgcatcacg gtggactcgg cccccgacat ggggacgacc ttcaccatcc acctgcccgc
     1561 cacaccacgg cccgacggag aagagacgga ttgagcgaga acctacagcc gcagagtgcg
     1621 ccgccgacgg cgaggatcct cgtcgccgaa gatgaatcga acctgcggct cgtcttacag
     1681 aaagagcttc agcggatggg ccacgaggtg cgcgtcgcgc ccgacggcga ggccgcgctg
     1741 cgcctgctgg aagagagcaa cgtggacgtg ctgctgacgg acatcaacat gccgcgcgtg
     1801 gacgggcttg agctgttgcg ccgcgtccac cagcgcccca acccgcccga ggtcatcatg
     1861 ctcaccggcc acgccacggt cgagagcgcc atcgaggcga tgaagctcgg cgcctacgac
     1921 tacctctcga agccctaccg catcgccgag ctggacgcgc tcgtcaagca ggccgccgag
     1981 aagcggcgtc tgcgcgtgga caaccagcgc ctgcgcacgc tcgtcgagcg gcagaccgcg
     2041 gcgcccgaga tcgtcttcgt ctccgacgtg atgcgcgagg tgatgcggct cgtcgagcgc
     2101 gtggcgccgt cggacgcctc cgtcctcatc acgggcgaga gcggcacggg caaggagctg
     2161 atcgcgcacg ccatccacaa gctctcgcgc cgcgccgaag gctcgttcat cgacctgaac
     2221 tgcgcggcgt ttcaggaggc gctcttggag tcggagctgt tcggctacga ggcgggcgcg
     2281 ttctccggcg cgaaggggcg caagctcggt ttgtgggagc tggccgacaa cggcacgatc
     2341 ttcctcgacg agatcacgga gctgccgtcg cccttgcagt cgaagctgct gcgcgccatc
     2401 gagacgaact cgttctaccg cgtcggcgcc gtgcgcaagg tgcaggtcaa cgtgcgcatc
     2461 gtcgcggcga ccaaccgcga cgtgaacgag gtggtcggcg acggtcgctt ccgcgcagac
     2521 ctgctctacc gcgtcaacgg cttccagatt cacctgccgc ccttgcgcga ccgcgccgaa
     2581 gacatcgagc cgatcacgcg ccacctgctc cgccagatgg gcggcgcgca cccgcccgag
     2641 gtcttgcccg aagccctcgc ggccctcaag gcgtaccact ggcccggcaa cgtgcggcag
     2701 ctccgcaaca cgctcgaacg cgccatcctg ctctcgaacg acgcgctcat caccgccgcc
     2761 gacctgccgc ccgaactgtc gaaccgcgcg cccgtctaca cgccggcctt cgcgcccatg
     2821 tctccgggcg gcccgctcgg ctcggtcatg aacgccgccg gccagcccgc aggcgacgtg
     2881 cccgcgccgc tacgcgaggt cgagcgccag cagattctcg ccgccctcga acagaccaac
     2941 tggcaccgcg gcaagaccgc cgaaatcctc ggcatctcgc cctccaccct ctaccgccgt
     3001 ctgcgcgact acaacctcac gcgtcgtcca tgaagaagag taggcagcag gcagtggaag
     3061 acagaagaaa gcaggaggct tgagacgcta tgtctcaagc ctcctgcttt ccagcttgaa
     3121 aagtggagag gctcgggact ttacctccca agcctcttct ttgttctgtc ttctgttttt
     3181 actgcctgct gccgactgcc gactgctctt catcccccgc cgccggtcgt ggtcttctgc
     3241 tcgttgcggg gtttgccgag ggagcggcgg gcggtgtcgg ctttggtggg ctggcctgtg
     3301 cgttcgtaga tggcgacgag gcgttcgagc agcgcgttgg tgccgggctg gaggcggagc
     3361 gcggcctcgt agtgtttggc ggcttcacgg tcgcgcttga cggtttcgag gatgtcggcg
     3421 agcttgacgt gcgcgtcgta ctcgccgccc gcgcgcgcga agctcttgaa ggcgtcgccg
     3481 tatttgccgg tgcgcgcctg cacgacgccg aggttgttgt ggatgacggc ggtcgcgggc
     3541 gacaggcgcg cagcctgctt gagccgcttc atggccgcgc cgtagttcat cgccgcgtag
     3601 tgcgagttgc cgaggttggc gagcacgacg gcgtcgtccg gcttgacgga gagggcacgc
     3661 tcgtaagcct cgatgg
//
LOCUS       DBNGOH010000590         4496 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_270776, whole genome shotgun sequence.
ACCESSION   DBNGOH010000590 DBNGOH010000000
VERSION     DBNGOH010000590.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4496)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4496)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4496
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_270776"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1363)
                     /locus_tag="AC159P_11425"
     CDS             complement(<1..1363)
                     /locus_tag="AC159P_11425"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012335.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HIR3696101.1"
                     /translation="MKQLLQNRRTGEVEVADVPAPAVARGRVLVRAAASLISAGTERS
                     AVGQNRKGLLGRALDQPELVRKTIRKALDEGVASTVGAVRAKLDSAVALGYSAAGVVA
                     EVGAGVEGFGAGDRVACAGLGYASHAEVLSVPKNLCARLPEGVSFADGAYATLGAIAL
                     QGVRLAQPTLGESVVVVGLGLLGQLAAQLLRANGCRVYGVDPDERRLELARALGADAG
                     CAPDEEAAAKIAEWTRGRGADAVLITAGTESSRPIVLAGEASRMKGRVVVVGQVGMDV
                     PRDLYYRRELSLQVSMSYGPGRYDPDYEERGHDYPLAYVRWTEARNLEAFLDMVARGS
                     VNVARLTTHRFKVEEGARAYELITGEREEPYLGVLVEYDAERETERRVELKPLAARAG
                     VEHEGAAVSTRSAGSPASVVRVGLVGAGGYAAGVLLPQFRAAGAEFETIASASGVSAL
                     NVGK"
     gene            complement(1396..1557)
                     /locus_tag="AC159P_11430"
     CDS             complement(1396..1557)
                     /locus_tag="AC159P_11430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696102.1"
                     /translation="MAKCPYCARPLRMGGIACRACRRYVLRWPHIVILVALCLAVVVG
                     VLELWFSLS"
     gene            1758..2213
                     /locus_tag="AC159P_11435"
     CDS             1758..2213
                     /locus_tag="AC159P_11435"
                     /inference="COORDINATES: protein motif:HMM:NF042704.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-phosphate processing receiver
                     domain-containing protein"
                     /protein_id="HIR3696103.1"
                     /translation="MGVLQGILNRLGLHELGQRRAPLRVMLLDDDVLRHEWFAKQFRG
                     DALDTAIDPARAVELLSANYYDLIFLDHDLLPEHYYAEGNDDERTGYAVAHWLASRPD
                     RQGAASIVVHTRNADGALRMVELLRRSGRQAEHVPFHLLTSRIKNYRGR"
     gene            2223..3482
                     /locus_tag="AC159P_11440"
     CDS             2223..3482
                     /locus_tag="AC159P_11440"
                     /inference="COORDINATES: protein motif:HMM:NF013696.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HIR3696104.1"
                     /translation="MRSRLLRITLLSLAVTIPLLASRSFAQAGAQYDAAVKAVMARAE
                     VQAAFAHVEASREEILREWVAITEVNAPSGKERERAELVERILRRQKSVDVRYDSAGN
                     LVAVRKGTGGGPAVVLDAHLDTVFPEGLKIKAELRDGRLYAPGVGDDTRNVVAMLATL
                     RALDAANVSTKGDLVFLFTVEEETTFKGVEEYVKENKAKIGHYIALDGGYEGLTYGGI
                     GINWYKHHFVGPGGHTRSRTPPYSATLPAARAIARIYALAVPDNPPSHVNIGMIGGAD
                     AVNAKAEDAWFTVDLRSTDNAVIADLERKIAAIVKEEAAREKMTVKTERLSSTPAVQV
                     PGHRESTLVKTAEAVHYAMGFKPSVTVTGSNNSNVALLAGVSAISTGAAPCGDSHSLT
                     EFCEVEPIYKGIKKVLLLAVAVAQISQ"
     gene            complement(3568..>4496)
                     /locus_tag="AC159P_11445"
     CDS             complement(3568..>4496)
                     /locus_tag="AC159P_11445"
                     /inference="COORDINATES: protein motif:HMM:NF015587.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HIR3696105.1"
                     /translation="SQLISKTVTDVTQGSGGGKLSFGILATLWAASNGMGAISETLNV
                     AYEVKETRPFWRVRLTAIGLTLALAFLIVSALVLVLYGHDLAELVAGRFGLGEAFEWA
                     WKILQWPIVLAFVLLAFALIYYFAPDLHDQEWKWVTPGSVLGVALWLLVSFGFKAYLS
                     YFNSYSATYGSLGAVIILMLWFYFTGAAILVGGEINSEIENEMAKRGHPDAKEKGQKA
                     PDGPVDKPQTAADAKPSSDPKPSPAKPPDDKPTGKATPAHARASQAPARATATRKGTG
                     ERRSLSFGKLAVVAGAWALSKLTGKKKVSSKR"
BASE COUNT          730 a         1636 c         1462 g          668 t
ORIGIN      
        1 gcttgccgac gttcaaggcc gacacgcccg acgcgctcgc aatcgtctcg aactccgcgc
       61 ccgccgccct gaactgcggc agcaacacgc ccgccgcgta accgcccgcg cccactaacc
      121 cgacgcgcac gaccgacgca ggcgaaccgg ctgaccgagt agaaacagcg gcgccttcat
      181 gctcgacgcc tgcccgcgcg gccaacggct tcagctcgac gcgcctctcc gtctcgcgct
      241 ccgcgtcgta ctcgacgagc acgccgaggt acggctcttc gcgttcgccc gtgatgagtt
      301 cgtacgcgcg cgcgccctct tcgaccttga agcggtgcgt cgtgagacgc gcgacgttga
      361 ccgagccgcg cgcgaccatg tcgaggaagg cttcgaggtt gcgcgcctcc gtccagcgca
      421 cgtaggccag cgggtagtcg tggccgcgct cttcgtagtc cgggtcgtag cggcccggcc
      481 cgtaagacat cgagacctgc aacgacagct cgcgccggta gtagaggtcg cgcggcacgt
      541 ccatcccgac ctgcccgacg accaccacgc gacccttcat ccgcgaagct tcgcccgcga
      601 gcacgattgg ccggctcgac tccgtgcccg ccgtgatcag caccgcgtcc gccccgcgcc
      661 cgcgcgtcca ctccgcaatc ttcgccgccg cctcttcgtc cggcgcacag cccgcgtccg
      721 cgccgagcgc gcgcgccagc tccagccttc tctcgtccgg atcgacgccg tagacgcggc
      781 agccgttcgc gcgcagcagt tgcgccgcga gctggccgag cagcccgagt ccgacgacga
      841 cgaccgactc gccgagcgtc ggctgcgcga gccgcacgcc ctgcaaggcg atggcgccga
      901 gcgtcgcgta cgcgccgtcc gcgaagctca cgccctcggg cagtcgcgcg cacaagttct
      961 tcgggacgga caacacctcc gcgtgcgaag cgtagcctaa cccggcgcac gccacgcggt
     1021 ctcccgcgcc gaacccttcc acgcccgcgc ccacttcggc gacgacgccc gccgcgctgt
     1081 agccgagcgc gaccgccgag tcgagcttcg cgcgcacggc gccgacggtg ctcgcgacgc
     1141 cctcgtcgag cgccttgcga atcgtcttcc tgaccagctc cggctggtcg agcgcgcggc
     1201 ccagcagccc cttgcggttc tggccgacgg cggagcgctc cgtccccgcg ctgatgagcg
     1261 aggcggcggc gcgcacgagc acgcgcccgc gcgcgaccgc cggcgcgggc acgtccgcga
     1321 cctcgacttc gcccgtccgc cggttttgta gaagctgttt catccgggag cgaaccgctc
     1381 cgcgcacgcg cgtcgtcaac tcagactgaa ccacagttcg agcaccccga ccacgactgc
     1441 gagacacagc gcgacgagga tgacgatatg cggccagcgt aggacgtagc ggcggcaggc
     1501 acggcaggcg atgccgccca tccgcaacgg cctcgcgcaa tagggacact ttgccatcgt
     1561 tcgattgcgg acgcgagggt gtcggcaaca atgccacgaa cgcttgaggc gaggcaagcc
     1621 gccgcgcccg cccccgcgcc ccccgactcg ccgcgcgccc ccgccccgtt gtgttatagt
     1681 ttttgcacgt cgcttcagct cggaatttct ttgccggcgg gtcgctgtgt cggacgccgc
     1741 cgaggaatcg aaagaccatg ggtgtcctgc aaggaatact gaacaggctc ggcctccacg
     1801 aactggggca gcggcgcgcg ccgctgcgcg tgatgctcct cgacgacgac gtgctgcgcc
     1861 acgagtggtt cgccaagcag ttccggggcg acgcgctcga cacggccatc gacccggcgc
     1921 gcgccgtcga gctgctctcc gcgaactact acgacctgat cttcctcgac cacgacctgc
     1981 tgcccgagca ctactacgcc gaaggcaacg acgacgagcg caccggctac gccgtcgccc
     2041 actggctcgc ctcgcgcccc gaccggcagg gcgcggcctc catcgtcgtc cacacgcgca
     2101 acgcggacgg cgcgctccgc atggtcgaac tgctccggcg ctccggtcgg caggccgagc
     2161 acgtcccctt ccacctgctc acgtcgcgga tcaaaaatta ccgagggcgg taacgaacac
     2221 tcatgagatc aagactcctc cgcatcaccc ttctctcact cgccgtcacc atccccttgc
     2281 tcgcgtcccg ttcgttcgcg caggcgggcg cgcagtacga cgcggcggtg aaggccgtca
     2341 tggcgcgcgc cgaggtgcag gcggcgttcg cgcacgtcga ggcgagccgc gaagagatac
     2401 tgcgcgagtg ggtggccatc acggaggtca acgcgccttc gggcaaggag cgcgagcgcg
     2461 ccgagctggt cgagcggatt ctgcggcggc agaagtccgt ggacgtgcgc tacgactcgg
     2521 cggggaacct cgtcgccgtg cgcaagggca cgggcggcgg cccggcggtc gtcctcgacg
     2581 cgcacctcga cacggtcttc cccgaaggct tgaagatcaa ggcggagctg cgcgacggga
     2641 ggctctacgc gcccggcgtc ggcgacgaca cgcgcaacgt cgtggcgatg ctggcgaccc
     2701 tgcgcgcgct cgacgccgcg aacgtcagca ccaaaggcga cctcgtcttc ctcttcacgg
     2761 tcgaggagga gacgaccttc aagggcgtcg aagagtatgt caaagagaac aaggcgaaga
     2821 tcggccacta catcgcgctc gacggcggct acgagggcct cacctacggc ggcatcggca
     2881 tcaactggta caagcaccat ttcgtcggcc ccggcggcca cacgcgctcg cgcacgccgc
     2941 cctactcggc cacgctcccg gcggcccgcg ccatcgcgcg catctatgcg ctcgccgtgc
     3001 ccgacaaccc gccctcgcac gtcaacatcg gcatgatcgg cggcgccgac gccgtcaacg
     3061 ccaaggccga ggacgcgtgg ttcaccgtcg atctgcgctc gaccgacaac gccgtcatcg
     3121 ccgacctcga aaggaagatc gcggccatcg tcaaggaaga ggcggcgcgc gagaagatga
     3181 cggtgaagac cgagcggctc tcctcgacgc ccgccgtgca ggtgcccggc caccgcgagt
     3241 ccacgctcgt caagaccgcc gaggccgtcc actacgcgat ggggttcaag ccctcggtca
     3301 ccgtcaccgg ctcgaacaac tcgaacgtcg cgctgctcgc gggcgtctcg gccatctcga
     3361 ccggcgccgc gccctgcggc gactcgcact cgctcaccga gttctgcgaa gtcgagccga
     3421 tctacaaggg catcaagaag gtcttgctgc tcgccgtcgc cgtcgcgcaa ataagtcagt
     3481 gagcggtaag cagtaaagaa gaggcttgag acgtagcgtc tcaagcctct tcagctttac
     3541 gctcttctgt ttttaactgc tcactgctta ccgcttactg ctcactttct tcttgcctgt
     3601 cagtttcgac aaggcccacg cgcccgcgac gacggcgagc ttgccgaagc tgagcgagcg
     3661 gcgttcgccc gttcccttgc gcgtcgcggt cgcgcgcgcg ggcgcttgcg aggcgcgcgc
     3721 gtgggcgggt gtcgccttgc cggtcggctt gtcgtcgggt ggtttggcgg gcgagggttt
     3781 cgggtcggag gaaggcttcg cgtcggcagc cgtttgcggc ttatcgacgg gtccgtcggg
     3841 cgccttctgg cccttctctt tggcgtccgg gtggccgcgc ttcgccatct cgttctcgat
     3901 ttcggagttg atctcgccgc cgacgaggat ggccgcgccg gtgaagtaga accagagcat
     3961 gaggatgatg acggcgccga gcgagccgta ggtcgcgctg taggagttga agtaggagag
     4021 gtacgccttg aagccgaacg agaccaggag ccacagcgcg acgccgagca cggagccggg
     4081 cgtgacccac ttccactcct gatcgtgcag gtcgggcgcg aagtagtaga tgagcgcgaa
     4141 ggcgaggagc acgaaggcga gcacgatggg ccactgcaaa atcttccacg cccactcgaa
     4201 cgcttcgccg aggccgaacc ggcccgcgac cagctccgcc aggtcgtggc cgtagagcac
     4261 gagcacgagc gccgagacga tgaggaaggc gagcgcgagc gtcagcccga tggccgtcag
     4321 gcgtacgcgc cagaaggggc gcgtctcctt gacctcgtag gcgacgttga gcgtctcgct
     4381 gatggcgccc atcccgttcg aggcggccca cagcgtggcg aggatgccga acgagagctt
     4441 gccgccgccg ctcccctgcg tcacgtccgt cacggtcttg ctgatgagct gcgagg
//
LOCUS       DBNGOH010000591         9191 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1005688, whole genome shotgun sequence.
ACCESSION   DBNGOH010000591 DBNGOH010000000
VERSION     DBNGOH010000591.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 9191)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9191)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9191
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1005688"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..467
                     /locus_tag="AC159P_11450"
     CDS             <1..467
                     /locus_tag="AC159P_11450"
                     /EC_number="5.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013353.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047661 - amino-acid racemase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HIR3696106.1"
                     /translation="DELAAHSPVPLLSIVEVCAREARRRGLRRPALLGTRFTMDAPFY
                     PAVCARYGIDVVTPNDAERAWVHERYVGQLLKGEFRDDTRREFVSLVSRLREEESIDG
                     VILGGTELPLLLPARSIADVPALDTTALHVAAIVERLRQPDAPPPEARAPAR"
     gene            551..1123
                     /locus_tag="AC159P_11455"
     CDS             551..1123
                     /locus_tag="AC159P_11455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010911674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycolipid-binding domain-containing
                     protein"
                     /protein_id="HIR3696107.1"
                     /translation="MSTDILEQTILWRRLDSPGHDACGLSSSSEGRRLAGTAVFSSEG
                     QPCRLCYEVECDTSWRTRSARVAGWVGRTALKLDIEAMQGGRWAFNGTEQAELTGLVD
                     VDLGFTPATNLIQLRRLALGVGHAADAPTVYLNFPELTLDRLEHRYHRVAPDRYDYRA
                     PRFDYAGILQVSAVGFVTHYPGLWELEALG"
     gene            1168..2355
                     /gene="tgt"
                     /locus_tag="AC159P_11460"
     CDS             1168..2355
                     /gene="tgt"
                     /locus_tag="AC159P_11460"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013696694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HIR3696108.1"
                     /translation="MSGGALGWRVLAEDGEARAGLLRTRRGVVETPVFMPVGTLGSVK
                     AVRFEELEAEDLDARIILGNTYHLWLRPGPETIRACGGLHRFIGWGRALLTDSGGFQV
                     WSLGDLRRISEEGVEFRSHVDGRQCFLSPEVSMEVQAALGSDIVMAFDECAPGDATHE
                     TARGSLELTARWARRSRSRFDRLQEEGADAGRDGAGEGDGVSGRQALFGIVQGATFKD
                     LRRESLARTQEVGFDGYAIGGLSVGEAKPLMYEVVEELAPRMPREAPRYLMGVGTPED
                     LVESVARGVDMFDCVLPTRNGRTGQAFTSRGKLNIKNARWADDPRPLDAACRCAVCRR
                     HSRAYLRHLQLTGEMLAAILLTHHNLAFFLDTMRRVRQAIRSGEFTRFRREFTEGITS
                     GVE"
     gene            2528..2851
                     /gene="yajC"
                     /locus_tag="AC159P_11465"
     CDS             2528..2851
                     /gene="yajC"
                     /locus_tag="AC159P_11465"
                     /inference="COORDINATES: protein motif:HMM:TIGR00739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HIR3696109.1"
                     /translation="MSTLAFNLLQAQGGGGLGGMLVGLAPFILIFAVFYFLIIVPQRK
                     RQRELQEMVTQLKAGDRIITTGGIIATVATVRDKSLLVRSDKSVFEITRQAVASQYSD
                     EEKGT"
     gene            2982..4685
                     /gene="secD"
                     /locus_tag="AC159P_11470"
     CDS             2982..4685
                     /gene="secD"
                     /locus_tag="AC159P_11470"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HIR3696110.1"
                     /translation="MKKNLLQRVIIIAVVTLLALWVVIGPRHRPSLSDFTLAGINKTL
                     RENIRLGLDLKGGSHLVMRVQVEDYLKRITEQNVMIGVREAAKAQGYDVKDVRSEVSG
                     DTYRIILEANDASKLGEMRDELPKRVNDFGADVWASRVEGNRIIWEMREEAKNALSRR
                     AVEDALRIIDTRINAVGVAEPTLQSHGAENSRQILLQMPGIQDPERIKALLIGESRLE
                     LMKVVGQPYPGGPTTYPTEQAAIQSLGGQVPPNRKVLQYDERDEPTAAGEQPQPTPGQ
                     PTAWAVVETPAVVDGSELRSAEAVTQQGGDDQYQISFTLKPGGAEKFGAWTGANVNQY
                     MAVVLNDRIKSAPYIKSQITDQGQIDGNFTKESADDLALTLRSGALPARLVYIEERTV
                     GPSLGEDSIRAGVTASLIGLLLIIVFMVFYYRGAGLNAVVALLLNIVLTLAALIIFDA
                     TLTLPGIAGVILSLGMAVDSNVLIFERIREELRTGKTVSSAVAAGFDRAFTTIIDTHV
                     TTIVAALFLFVFGTTLIRGFAVTLVLSLIANLFSAVYVSRTIFIWLLSRKRTRAETLS
                     I"
     gene            4788..5933
                     /gene="secF"
                     /locus_tag="AC159P_11475"
     CDS             4788..5933
                     /gene="secF"
                     /locus_tag="AC159P_11475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HIR3696111.1"
                     /translation="MIRLLEDVNVDWLGKRKMFLIFSSLLLLAGLASAVGRTLAGKEP
                     FNLGVDFRGGTLVTVDFKQRPAPEAIRDELAKSGLGDATIQPALDKDNQVLIKIAQQP
                     GAGAPPGEPQAPTPQPTANAAAPSPQASAQPGASPTLSAEAQVDPGQKKVRDALKAFG
                     REGKLAEGADWQVSGMEAVGPVAGRQLRNQAVTVTLLALVGILLYIAFRFEWTYGASA
                     VITVFHDVLVTLAFFSIFQIEVNLTVVAALLTLVGFSVNDTIVTFDRVRENLRLRRRT
                     SLYDITNEAINQTLSRTIITSGLVFLSVVALVFFGGEVLRPFSLALLIGVIFGSYSTI
                     AISNPIMVWLQQRLERGTAAPTSAKPGGRQAPRRAPAGARNARAAAR"
     gene            6430..7251
                     /locus_tag="AC159P_11480"
     CDS             6430..7251
                     /locus_tag="AC159P_11480"
                     /inference="COORDINATES: protein motif:HMM:NF015506.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HIR3696112.1"
                     /translation="MFDSLVESGSHSDDIARKGSFFLGTFIFYAVGLVAAAVGGIYYA
                     NSSIPDPSLELLTLVAPVPVQQQQEQKQEQKQEQKPAAKDEPQVNTRTEISVVAPVVK
                     SNEVAKETTKEISRAKSAVTIVGTSDRDAPAGAVAPGPISGTGMGGGGVATGPAAPVV
                     VADDAPPPPPKPTPPPAPKKTIVSGGVLNGKAISKPQPPYPAIAKAARASGTVTVQIV
                     VDESGRVISASAVGGHPLLQQAAVQAARQARFSPTLLSGQPVKVSGVITYNFVLQ"
     gene            7406..8170
                     /locus_tag="AC159P_11485"
     CDS             7406..8170
                     /locus_tag="AC159P_11485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014267546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HIR3696113.1"
                     /translation="MSILGSKALSFFVFLWQEAGAGGGEDMAQHFTITEMVKNLGTVA
                     LIVLIILLIMSMYSIAIMVERFLTYSAAKKQSREFAPRVAQALKNDRIEEAINISDKH
                     KKSHLAMVVNAGLQEFRAHSNSQDISGEEIEASKRALQRAIAIKSAEFKRGLSGLATV
                     GSTAPFVGLFGTVFGIINAFTGMKSAESAGIGAVAGGIAEALIATAIGLGVAVPAVWA
                     FNYFSGKVDGFIIEMDNSASELIDYFLKNRTRQLKS"
     gene            8277..8768
                     /locus_tag="AC159P_11490"
     CDS             8277..8768
                     /locus_tag="AC159P_11490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014783985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ExbD/TolR family protein"
                     /protein_id="HIR3696114.1"
                     /translation="MGMGAGGDSGGLQSNINVTPMVDIMLVILIIFMVITPFLQQGVS
                     VALPRDMRNPDEDPAIIKETSVVVAITADDKIFLGKKPIEMAELKTEVDAKMSSKERD
                     QRIVYVRSDINTNYGTVVNVINTIRDAGVDQIGLVADKKKGGGAAADPAGGAPAPAPA
                     PGS"
     gene            8858..>9191
                     /locus_tag="AC159P_11495"
     CDS             8858..>9191
                     /locus_tag="AC159P_11495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014267544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HIR3696115.1"
                     /translation="MGMSTPSSGGAKPDINVTPFIDILLVLLIIFMVITPLKPTRFKT
                     LVPEKSEEMSAQAKQSPRTLIVAIDKTQKVTLTRGANVIADGAVEDPTNIATRLAAEF
                     KERRDQQIW"
BASE COUNT         1563 a         3090 c         3026 g         1512 t
ORIGIN      
        1 tcgacgaact cgcggcccac tcgccggttc cgctgttgag catcgtcgag gtctgcgcgc
       61 gggaagcgcg acgacgcggg ttgcgcaggc cggcgctcct cggcacgcgg ttcacgatgg
      121 acgcgccctt ctaccccgcg gtgtgcgcgc gttacggaat cgacgtggtg acgccgaacg
      181 acgcggagcg ggcgtgggtg cacgagcggt atgtgggaca gctcctcaag ggcgagttcc
      241 gcgacgacac gcggcgcgag ttcgtgtcgc tcgtcagccg cttgcgcgaa gaggagagca
      301 tcgacggcgt cattctcggc gggacagagc tgcctctgct cttgcccgcg cggtcgatcg
      361 cggacgttcc ggcgctcgac accacggcgc tgcacgtggc cgccatcgtc gagcgtctac
      421 gacaacccga cgccccgccg ccggaggccc gcgcgcccgc gcgctgacag aggcgcggcg
      481 gccaacgggc gagacgtgga agcgcgcagg gaaggcccgc gcgcttcttt ctccatccgc
      541 ggtgcgaggg atgagcacgg acatactcga acaaacgatt ttgtggcgca ggctggactc
      601 tccgggccat gatgcgtgcg gactctcttc gtcgagtgaa ggccggcgtc tggccgggac
      661 ggcggtcttc tcgagcgaag ggcagccgtg ccgcctgtgc tacgaggtgg agtgcgacac
      721 gtcgtggcgg acgcgctcgg caagggtcgc gggttgggtg ggacggactg cgttgaagct
      781 cgacatcgag gccatgcagg gtgggcgctg ggccttcaac ggtacggagc aggcggagct
      841 gacgggtctc gtcgatgtcg atctcgggtt cacccccgcc acgaacctga ttcagctccg
      901 gcgcctcgct ctcggcgtcg gccacgcggc tgacgccccg acggtctatc tgaacttccc
      961 cgagctgact ctcgacaggc ttgagcatcg ttaccatcga gtggcacccg accggtacga
     1021 ctatcgagcg ccccgtttcg attacgcggg aatcctgcaa gtgtcagccg tcggcttcgt
     1081 cacgcactat cccggtctgt gggagttgga ggcgctcgga tgactgaaaa ataccccgag
     1141 cctgtgagcc gccgccgggc aacggccatg agcggagggg cgctcgggtg gcgggtcttg
     1201 gccgaggacg gggaggcgcg ggcggggctt ctgcggacgc ggcgcggcgt ggtcgagacg
     1261 ccggtgttca tgcccgtggg cacgctcggt tcggtgaagg ccgtgaggtt cgaggagctg
     1321 gaggcggaag acctcgacgc gcggatcatc ctcggcaaca cgtatcacct gtggctgcgg
     1381 cccggcccgg agacgatcag ggcgtgcggc ggtctgcacc gtttcatcgg ttgggggcgc
     1441 gcgttgctga cggactcggg gggctttcag gtgtggagcc tcggcgactt gcggcgcatc
     1501 tcggaggagg gcgtcgagtt ccgctcgcac gtggacggca ggcagtgctt tctgtcgccg
     1561 gaggtctcga tggaggtgca ggccgcgctc ggctccgaca tcgtgatggc cttcgacgag
     1621 tgcgcgccgg gcgacgcgac gcacgagacg gcgcgcggct cgctcgaact gacggcgcgc
     1681 tgggcgcggc gctcgcgctc gcgcttcgac cgcttgcagg aagagggcgc ggacgcgggc
     1741 cgcgacggcg cgggcgaagg ggacggcgtg agcggtcggc aggcgctctt cggcatcgtg
     1801 cagggcgcga ccttcaagga tttgcggcgg gagagtctgg cgcgcacgca ggaggtcggc
     1861 ttcgacgggt acgctatcgg gggcctgagc gtcggcgagg cgaagccctt gatgtacgag
     1921 gtggtggaag agttggcgcc gcggatgccg cgcgaggcgc cgcgctacct gatgggcgtg
     1981 gggacgccgg aggacctggt cgagtccgtg gcgcgcggcg tggacatgtt cgactgcgtg
     2041 ctgccgacgc gcaacggtcg gacggggcag gcgttcacct cgcgcggcaa gctcaacatc
     2101 aagaacgcgc ggtgggccga cgacccgcga ccgctcgacg cagcttgccg gtgcgccgtc
     2161 tgccgcagac attcgcgcgc ctacctgcgc catttgcagt tgacggggga gatgctggcc
     2221 gcgatcctcc tgacgcacca caaccttgcg ttcttccttg acaccatgcg gcgcgtgagg
     2281 caagctatcc ggtctggcga attcacacga tttcggcggg agttcaccga aggaatcacc
     2341 tccggcgtcg agtgagccgc gcctctcgtg cgaagccggc gccgccggtc tcttccaact
     2401 gataacagac atttcagttg aagtcgcgga gggcggtctc aggccgttgg aggcgcgccc
     2461 cgcgggcgtc tttttgcgtt aaaagagggg ttcgaccccg acgactccga cagtcaccaa
     2521 tcaaccaatg tccactttag ctttcaacct tttgcaagcg cagggcggcg gcggcctcgg
     2581 cgggatgctc gtcggcctcg cgccgttcat cctgatcttc gccgtcttct actttctcat
     2641 catcgtcccc cagcgcaaac gacagcgcga gctacaggag atggtcacgc agctcaaggc
     2701 gggcgaccgc atcatcacga ccggcggcat catcgccacg gtcgccacgg tgcgcgacaa
     2761 gtcgctgctc gtccgcagcg acaagtccgt cttcgagatc acgcgccagg ccgtcgccag
     2821 ccagtactcg gacgaggaga agggaacatg aatagaagtc agaagccgga agtcagaagt
     2881 cagaaggccc cgcgcgggcg cgctgacgct gacgacatcg gctctcggcc acggtagttt
     2941 tttgtgctga cttctgactt ccgacttctg acttctgctt tatgaaaaag aatcttcttc
     3001 agcgcgtcat catcatcgcg gtcgtgacgc ttctcgcgct gtgggtcgtg atcgggccgc
     3061 gccaccgccc gtcgctctcg gacttcacgc tggccggcat caacaagacg ctgcgcgaga
     3121 atatccgcct cggcctcgac ctcaagggcg gctcgcacct cgtcatgcgc gtccaggtcg
     3181 aggactacct caagcgcatc accgaacaga acgtcatgat cggcgtgcgc gaagcggcga
     3241 aggcgcaggg ctacgacgtg aaggacgtgc gcagcgaggt gagcggcgac acgtaccgca
     3301 tcatcctcga agcgaacgac gcctcgaagc tgggcgagat gcgcgacgag ctgccgaagc
     3361 gcgtcaacga cttcggcgcc gacgtgtggg cttcgcgcgt ggagggcaac cgcatcatct
     3421 gggagatgcg cgaggaggcc aagaacgccc tgagccgccg cgccgtcgag gacgcgctcc
     3481 gcatcatcga cacgcgcatc aacgccgtcg gcgtcgccga gccgaccttg cagtcgcacg
     3541 gggccgagaa ctcgcgccag attctcctcc agatgcccgg catccaggac ccggagcgca
     3601 tcaaggcgct cctcatcggc gagtcgcggc tggagctgat gaaggtcgtc gggcagcctt
     3661 accccggcgg gccgaccacc tacccgaccg agcaggcggc catccagtcg ctcggcgggc
     3721 aggtgccccc gaaccgcaag gtgctccaat acgacgagcg cgacgagccg accgccgccg
     3781 gggagcagcc ccagccgacg ccgggccagc cgaccgcgtg ggcggtcgtc gagacccccg
     3841 cggtcgtgga cggcagcgag ctgcgcagcg ccgaagccgt cacgcagcag ggcggcgacg
     3901 accagtacca gatcagcttc accctgaagc ccggtggcgc cgagaagttc ggcgcgtgga
     3961 cgggcgccaa cgtcaaccag tacatggccg tcgtcctcaa cgaccggatc aagtcggcgc
     4021 cctacatcaa gtcgcagatc accgaccagg ggcagatcga cggcaacttc accaaggagt
     4081 cggcggacga cctcgcgctc acgctgcgct cgggcgcgct cccggcgcgg ctcgtctaca
     4141 tcgaagagcg caccgtcggg ccgagcctcg gcgaggactc gatccgcgcg ggcgtcacgg
     4201 cctcgctcat cgggctgctg ctcatcatcg tcttcatggt cttctactac cgcggggcgg
     4261 ggctgaacgc ggtcgtcgcg ctgctcctca acatcgtgct cacgctcgcg gcgctcatca
     4321 tcttcgacgc gacgctgacg ctgcccggca tcgccggcgt catcctgagc ctgggcatgg
     4381 cggtggactc gaacgtgctc atcttcgagc gcatccgcga agagctgcgc accggcaaga
     4441 ccgtctcgtc ggcggtggcc gccgggttcg accgcgcgtt caccacgatc atcgacacgc
     4501 acgtgacgac catcgtcgcg gccctcttcc tcttcgtctt cgggacgacg ctgatccgcg
     4561 gcttcgccgt cacgctcgtc ctgagcctga tcgccaacct cttctcggcg gtctacgtct
     4621 cgcgcaccat cttcatctgg ctcctcagcc ggaagcggac gcgcgccgag acgctcagca
     4681 tttaaccgcc gtgaaccgta aaacgtaaac cgtgatcagg gaagaaccgg ttttcactca
     4741 tcacggttga cggtttacgg ttcacgattt acgattcacg gtttcctatg attcgcttat
     4801 tagaagacgt aaatgttgac tggctgggca agcggaagat gttcctgatc ttctcgtcgc
     4861 tgctgctgtt ggcgggcctc gcgtccgccg tcgggcggac gctggccggc aaggagcctt
     4921 tcaacctcgg cgtggacttc cgcggcggca cgctcgtcac ggtcgatttc aaacagcgcc
     4981 ccgcgcccga ggccatccgc gacgagctgg ccaagtccgg cctcggcgac gccaccatcc
     5041 agcccgcgct cgacaaggac aatcaggttc tcatcaagat cgcccagcag cccggcgccg
     5101 gcgcgccgcc gggcgagccg caggccccca cgccgcagcc gacggccaac gccgccgcgc
     5161 cctcgccgca ggcttcggcg cagccgggcg cttcgcccac actgagcgcg gaggcgcagg
     5221 ttgaccccgg ccagaagaag gtccgggatg cgctcaaagc cttcggcaga gagggcaagc
     5281 tggcggaagg ggccgactgg caggtctccg gcatggaggc ggtcggcccg gtcgcgggcc
     5341 gccagttgcg caaccaggcc gtgaccgtga cgctgctggc gctcgtcggc atcctcctct
     5401 acatcgcctt ccgcttcgag tggacgtacg gcgcctccgc cgtcatcacc gtcttccacg
     5461 acgtgctggt gacgctcgcc ttcttctcga tcttccagat cgaggtcaac ctgacggtgg
     5521 tggccgcgct cctgacgctc gtcggcttct ccgtgaacga caccatcgtc accttcgacc
     5581 gcgtccgcga gaacctgcgc ctgcgccgcc gcacctcgct ctacgacatc acgaacgagg
     5641 ccatcaacca gacgctctcg cgcacgatca tcacgtcggg gctggtcttc ctgtcggtcg
     5701 tcgcgctcgt cttcttcggc ggcgaggtct tgcgcccctt ctcgctcgcg ctgctgatcg
     5761 gcgtcatctt cggcagctac tcgaccatcg ccatctccaa cccgatcatg gtctggttgc
     5821 agcagcgtct cgaacgcggc accgccgcgc cgacctccgc caagcccggc ggccgccagg
     5881 cgccgcgccg cgcccccgcc ggcgcgcgca acgccagagc cgccgcacgg taaagacagc
     5941 gaggcgaaag ggcgaaaagg cgaagagggg aagaaggatg atgggaggca cgctgcttct
     6001 cattctccct tttcccctct tgaccttttc gccctttcgc cctatgccct ttgcctttcc
     6061 cgcttttttt ccgcttgact tcgcgcgtcc ttgcacctta gactgagcgc gttgctggcc
     6121 aatcgaatca tttgaacgcg cccgtcctac gccgaggtgt acggacggac gtttgagcgt
     6181 gagcgtttct acgtctttaa gtgattggcc gggtcattcc ctgtggcaag cgtcgggtgt
     6241 ggtgattaaa agcacgctca gaggcgcacg gatggacgct ctgccgcccc ccaatcttta
     6301 ggacaattcg cgcttacaag cgggcgtttt aattgaaaac gttttagaat cacaaccctt
     6361 tttacaaggt ttttgtgatt aactctacgc gcccctgatc cacctggcac tggaagaggg
     6421 aggaccatca tgttcgacag tctggttgag tccggttcgc actccgacga catcgcgcga
     6481 aaaggctcat tctttctcgg cacgttcatc ttctacgccg tcggcctcgt cgccgcggcg
     6541 gtcggcggca tttactacgc caactcctcg atccctgacc cgagcctcga actgctgacg
     6601 ctggtcgcgc ccgtgcccgt ccagcaacag caggagcaga agcaggagca gaagcaggag
     6661 cagaagcccg ccgccaagga cgagccgcag gtcaacacgc gcacagagat ttcggtcgtc
     6721 gcgcccgtcg tcaagagcaa cgaggtggcg aaggagacga ccaaggaaat ttcccgcgcc
     6781 aagagcgccg tgaccatcgt cggcaccagt gaccgcgacg ccccggcggg cgccgtggcc
     6841 cccggcccga ttagcggcac cggcatgggc ggcggcggcg tcgccaccgg ccccgccgcg
     6901 cccgtcgtgg tcgccgacga cgcgccgccc ccgccgccca aaccgacgcc cccgcccgcg
     6961 ccgaagaaga ccatcgtctc gggcggcgtg ctgaacggca aggcgatcag caagccgcaa
     7021 ccgccctacc cggcgattgc caaggccgcg cgcgcctccg gcaccgtcac cgtccagatc
     7081 gtcgtggacg agtcgggtcg agtcatctcg gcctcggcgg tgggtgggca cccgctcttg
     7141 cagcaggcgg cggtgcaggc cgcgcggcag gcccgcttct cgccgacgct gctttccggg
     7201 caacccgtga aggtctccgg cgtcatcaca tacaacttcg tgcttcagta aactgggcgc
     7261 ggggcgcgac ccacagcaga gcggggctgc cggtgtgcgg cggccccgcc gtcgccccaa
     7321 cgggtcgcac gcgccccttc gcgtaagcgc tctcactctc tggcgtgatt gaaaactttt
     7381 ggaggttctt accgtgacca ttctcatgag cattctcgga agcaaggcgc ttagcttctt
     7441 cgtgttcctg tggcaggagg ccggggcggg cgggggcgag gacatggccc agcacttcac
     7501 cattaccgag atggtgaaga acctcggcac ggtggccctc atcgtgctca tcatcctgct
     7561 catcatgtcg atgtactcca tcgccatcat ggtcgagcgc ttcctgacct actcggcggc
     7621 caagaagcag tcgcgcgagt tcgcaccgcg cgtcgcgcag gcgctcaaga acgaccgcat
     7681 cgaggaggcc atcaacatca gcgacaagca caagaagtcg cacctcgcga tggtcgtcaa
     7741 cgccggcctg caagagttcc gcgcgcacag caactcgcag gacatctcag gcgaggagat
     7801 cgaggcttcg aagcgcgccc tgcaacgcgc catcgccatc aagagcgccg agttcaagcg
     7861 cggcctctcg ggcctcgcca cggtcggctc gaccgccccg ttcgtcggcc tgttcggcac
     7921 ggtgttcggc atcatcaacg ccttcaccgg catgaagtcg gcggagagcg cgggcatcgg
     7981 cgcggtcgcg ggcggcatcg ccgaggcgct catcgcgacg gccatcggcc tcggcgtggc
     8041 ggtgcccgcc gtgtgggcgt tcaattactt ctcgggcaag gtcgatggct tcatcatcga
     8101 gatggacaac tcggcgtcgg agctgatcga ctacttcctg aagaaccgca cgcggcagct
     8161 caagtcgtag gccgaaggct tctcgcgggg cggcgcccgg cgtcgcgcgc taaagggttc
     8221 acggcccggc ggcgcgccgc cgggccaccc cggcacgttc gtaaggagat aaagacatgg
     8281 gcatgggagc aggcggcgac agcggcggcc ttcagtcgaa catcaacgtc acgccgatgg
     8341 tggacattat gctcgtcatc ctcatcatct tcatggtgat tacgccgttc ctccagcagg
     8401 gcgtctcggt cgcgctcccg cgcgacatgc ggaacccgga cgaggacccg gccatcatca
     8461 aggagacttc ggtcgtcgtc gccatcacgg cggacgacaa aatcttcctc ggcaagaagc
     8521 cgatcgagat ggccgagctg aagaccgagg ttgacgcgaa gatgtcgagc aaggagcgcg
     8581 accagcggat cgtctacgtc cgcagcgaca tcaacaccaa ctacggcacg gtcgtgaacg
     8641 tgatcaacac catccgcgac gccggggtcg accagatcgg gctggtggcc gacaagaaga
     8701 agggcggcgg ggcggcggcg gatccggcgg gcggcgcccc ggccccggcc ccggcgcccg
     8761 gctcgtgaac gacggaacct tttcgcgggc gccgcgcttt ttgcctgacc gcaaccgcgc
     8821 gggcgcccgc cttcacgccg aaggagtcta agtcaacatg ggaatgagca cgccgtcgtc
     8881 cgggggcgcc aagccggaca tcaacgtcac gccgttcatc gacatcctgc tggtgctgct
     8941 tatcatcttc atggtcatca cgccgctgaa gccgacgcgc ttcaagacgc tcgtgcccga
     9001 gaagagcgag gagatgagcg cgcaggccaa acagagtccg cgcacgctga tcgtcgccat
     9061 cgacaagacc cagaaggtga cgctgacacg cggggcgaac gtgatcgccg acggcgccgt
     9121 cgaagacccc accaacatcg ccacgcggct ggcggcggag ttcaaggagc gccgcgatca
     9181 gcagatctgg a
//
LOCUS       DBNGOH010000592         3400 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4853484, whole genome shotgun sequence.
ACCESSION   DBNGOH010000592 DBNGOH010000000
VERSION     DBNGOH010000592.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3400)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3400)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3400
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4853484"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1513)
                     /gene="larAH"
                     /locus_tag="AC159P_11500"
     CDS             complement(<1..1513)
                     /gene="larAH"
                     /locus_tag="AC159P_11500"
                     /EC_number="5.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011551640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NPN-dependent hydroxyacid isomerase,
                     LarAH14/15/16 family"
                     /protein_id="HIR3696116.1"
                     /translation="MSLQLRHKVHESVVYIDRDSAPRIVTSGEDFLLEDLPIGTRVIY
                     PKPPIKGLPNREAAIRYALNHPHDCEPLYAQLEPGMKVTIAVDDISLPLPPMATPDIR
                     QTVLEVVLDLLAANGVDDVHIIIANSLHRKMTAWEMKRMVGAEIYDEFYPERYYNHDA
                     EDPDGMVVLGETRHKEPLRMNRRCAESDLVIYCNINLVPMDGGHKSVAVGLCDYESLR
                     AHHEPQTIRDSDSYMDPARSELSHKVARLGKLVDEHMNVFHIETAINNRMYKGEMDFL
                     LKNEDEFSAFDRLKFEAVRRTMSALPRAARRKLLHSFPAAYEMTACHAGRTEPAHAKI
                     LEKNFDQYAIPVTGQCDILICPVPDISPYNVYSILNPLLVQVMALGYHFNFYRNKPLL
                     KKGGVLILHHPCYDEFDHNFHPSYIEFFNRLLPETRDADTLRDKYEREFANNPSYIEM
                     YRRGNAYHGAHPFFMWYWGENGRQHVGKIIAVGADNEHVPRMMGWDSAETLSEA"
     gene            complement(1589..2251)
                     /locus_tag="AC159P_11505"
     CDS             complement(1589..2251)
                     /locus_tag="AC159P_11505"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR3696117.1"
                     /translation="MAAAAFYDLEGTLVSTNLVHTLGFYARNQQGLLRSVRKSASTLV
                     SLPLFAAADQYSRIFFNDLFFKRYKGETEDRLRFFADELFEEVLKPGVFPGAFELIQK
                     SKSLGLRQVVVTGALDMSVKPLMDYLGIEDYAANHLEFVNGVATGRLLPPVLASATKA
                     SWIRAFAEREKINLNDSYAYSDSMSDLPMLSLVGHPAAVNPDMRLRQTALHHDWPILN
                     LK"
     gene            2430..3230
                     /locus_tag="AC159P_11510"
     CDS             2430..3230
                     /locus_tag="AC159P_11510"
                     /inference="COORDINATES: protein motif:HMM:NF028121.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter TatT component family protein"
                     /protein_id="HIR3696118.1"
                     /translation="MTPKRPHFHTGLIPPLTFLLLFALSCASCTRPAEPAPAPPPGPT
                     APELIAQAEALYAQRDSLERVREAVSLMRRARATDNQSYEAAWKLSKFSYYLGEYGED
                     EGVRTEAFREGIAAGQSAIALDPNKPEGHFWLGANLGGRARLQGPIYALSSVPDIRRE
                     METVVRLDEGFQGGSAYMALGQIELELGMLGGDPKRAVEYLEKGLQFGETNTMLRLRL
                     AEAYLAVNRDEDARRQLNAIIKTTPNPDYAQEHKRAVEEARKLLEKRL"
BASE COUNT          576 a          991 c         1194 g          639 t
ORIGIN      
        1 tggcctccga gagcgtctcg gccgagtccc atcccatcat gcggggcacg tgctcgttgt
       61 ccgcgccgac cgcgatgatc ttgccgacgt gctgcctccc gttctcgccc cagtaccaca
      121 tgaagaaggg gtgcgcgccg tggtaggcgt tgccgcggcg gtacatctcg atgtagctgg
      181 ggttgttggc gaactcgcgc tcgtacttgt cgcgcaacgt gtcggcgtcg cgcgtctcgg
      241 gcaggaggcg gttgaagaac tcgatgtagc tcgggtggaa gttgtggtcg aactcgtcgt
      301 agcaggggtg gtggaggatg agcacgccgc ccttcttcaa gagcggcttg ttgcggtaga
      361 agttgaagtg gtagccgagc gccatcacct gcacgaggag cgggttcagg atcgagtaga
      421 cgttgtaggg cgagatgtcg ggcacggggc agatgaggat gtcgcactgg cccgtgacgg
      481 ggatggcgta ctggtcgaag ttcttttcga gaatcttcgc gtgcgccggc tcggtgcgcc
      541 cggcgtggca ggcggtcatc tcgtaggcgg cggggaacga gtgcaggagc ttgcggcgcg
      601 cggcgcgggg cagggcgctc atggtgcggc gcaccgcctc gaacttgagg cggtcgaagg
      661 cggagaactc gtcctcgttc ttgaggagga agtccatctc gcctttgtac atccggttgt
      721 tgatcgccgt ctcgatgtgg aagacgttca tgtgctcgtc caccagcttg ccgaggcgcg
      781 cgaccttgtg gctcagctcc gagcgcgcgg ggtccatgta gctgtcggag tcgcggatgg
      841 tctgcggctc gtggtgcgcg cgcaaagatt cgtagtcgca cagcccgacg gcgaccgact
      901 tgtggccgcc gtccatcggg acgaggttga tgttgcagta gatgacgagg tcggattcgg
      961 cgcagcggcg gttcatccgc agcggctctt tgtggcgcgt ctcgccgagg acgaccatcc
     1021 cgtcggggtc ttcggcgtcg tggttgtagt agcgctcggg gtagaactcg tcgtaaatct
     1081 cggcgccgac catgcgcttc atctcccacg cggtcatctt ccggtgcagg gagttggcga
     1141 tgatgatgtg cacgtcgtcc acgccgttcg ccgccaagag gtcgaggacg acttcgagga
     1201 cggtctggcg gatgtcaggt gtcgccatcg gcgggagcgg caagctgatg tcgtccaccg
     1261 cgatggtgac cttcatgccc ggctcgagct gcgcgtagag cggctcgcag tcgtgcgggt
     1321 ggttcaaggc gtagcggatg gcggcctcgc ggttgggcaa gcccttgatg ggcggcttcg
     1381 ggtagatgac gcgcgtcccg atgggcaggt cttccaaaag gaagtcttcg cccgaggtga
     1441 cgatgcgcgg cgcgctgtcg cggtcgatgt agacgaccga ttcgtggact ttgtgtcgaa
     1501 gctgtaggct catcttttag taaatggtaa gtggtgaatg gtaaatggta agcagcgaaa
     1561 aacctattca ccatttacca tttactattc acttcagatt aagtatcggc cagtcgtggt
     1621 gcagagccgt ctgccggagg cgcatgtcgg ggttgaccgc cgccgggtgg ccgacgagcg
     1681 agagcatcgg caggtcgctc atgctgtcgc tgtaggcgta ggagtcgttc aggttgatct
     1741 tctcgcgctc ggcgaaggcg cgtatccacg aagccttcgt ggccgaggcg aggacgggcg
     1801 gcaagagtct ccccgtggcc acgccgttga cgaattcgag gtggttggcg gcgtagtctt
     1861 cgatgccgag gtagtccatc agcggcttga cggacatgtc gagcgcgccg gtgacgacga
     1921 cctgccggag gcccaagctc ttcgacttct ggatcagctc gaaggcgccg gggaagacgc
     1981 cgggcttgag gacttcctcg aacagctcgt cggcgaagaa gcggaggcgg tcttccgttt
     2041 cgcccttgta gcgcttgaag aagaggtcgt tgaagaagat gcggctgtac tggtcggcgg
     2101 cggcgaagag cggcaggctg acgagcgtcg aggcgctctt gcggacgctg cgcaagaggc
     2161 cctgctggtt gcgcgcgtag aagccgagcg tgtgcacgag gttcgtgctg acgagcgttc
     2221 cttcgaggtc gtagaatgcg gctgccgcca aagcagtcct ccgagaagcc cgacgcgggc
     2281 cgaagtccgt cgtgcgagct tgaaagtaaa atgcgatacc ggaatgcggt gcaaaactat
     2341 aacaccaaat cccacatttt tcgtagttgg gggtgctata cttcgtcgct ccccggcccg
     2401 ctccgcagtc gcagacgagg acgaaccact tgacgccgaa acgcccacac ttccacacgg
     2461 gcctgattcc gccgctcact ttcctgttac tcttcgcgct ctcctgcgcc tcctgcacgc
     2521 gccccgcgga gccggcgccc gccccgccgc ccggccctac ggcgccggag ttgatcgcgc
     2581 aggccgaggc gctctacgcg cagcgcgact ctctggagcg cgtgcgcgag gccgtgtcgc
     2641 tgatgcggcg ggcgcgcgcg acggacaacc agagttacga ggcggcgtgg aagctctcga
     2701 agttcagcta ctacctcggc gagtacggcg aggacgaggg cgtgcggacg gaggcgttcc
     2761 gcgaaggcat cgcggcgggc cagtccgcca tcgccctcga cccgaacaag cccgaagggc
     2821 acttctggct cggcgcgaac ctcggcgggc gcgcgcgttt gcaggggccg atctacgcgc
     2881 tctcgtccgt ccccgacatc cgccgcgaga tggagacggt cgtcaggctc gacgagggct
     2941 ttcagggcgg gagcgcctac atggcgctcg ggcagatcga gctggagctg gggatgctcg
     3001 gcggcgaccc gaagcgcgcc gtcgaatatc tggagaaggg tttacagttc ggcgagacga
     3061 acacgatgct gcggctccgg ctcgccgaag cctacctcgc cgtcaaccgc gacgaagacg
     3121 cgcgccgcca gttgaacgcg atcatcaaga cgacgcccaa ccccgactac gcgcaggagc
     3181 acaagcgcgc cgtcgaagag gcgcggaagc ttcttgagaa gaggctgtag agcagcgggc
     3241 agcgggcagc aggcagcggg cagtaaaagc aggaggcttg agagctaacg tctcaagcct
     3301 cctgctttgt tgctcgaagc gcggagaggc tcaggacttt acctctcaag cgtcttcgcc
     3361 cttctgtttt gactgcatac tgcccgctgc ccgctgcctg
//
LOCUS       DBNGOH010000593         7234 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2472192, whole genome shotgun sequence.
ACCESSION   DBNGOH010000593 DBNGOH010000000
VERSION     DBNGOH010000593.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7234)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7234)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7234
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2472192"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(65..1267)
                     /locus_tag="AC159P_11515"
     CDS             complement(65..1267)
                     /locus_tag="AC159P_11515"
                     /inference="COORDINATES: protein motif:HMM:NF024743.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HIR3696119.1"
                     /translation="MTKIRTIIFALLAALACHVPAPAQGDAPPVERDEEARTVRVRLE
                     RGAKVEVDNRTTGRIVVSGWDRDYVEAVATSERGREYVRAYSGRDPSGQRVSLTADYL
                     TPEKRLPNPPDEGPKSVPPAPAATPASPAQGGVEAKSQGPPAAARTNESVLAEAREKV
                     FEFLFEQRPSEVHLEVKLPRYAEIELIEVNRSEVEIEGVETPVAVNGRRSAIRLRRVG
                     AVAVRTASGEVEVEGAGGLVDVMTESGDVNVRHVRGDVRVLSLSGEIEVECVTGRVNV
                     NNTSGSVALARVGGDADATTVSGEIRFEGALRADGRYHLKSMSGAVEMWLAPNPPGFT
                     ALLSSYRGQIVDGFSLKKTLKSGPNVVNNRVLGRYGNGQAQVTLDSFDGRVKLVRAAP
                     GTTAECKQ"
     gene            complement(1324..2310)
                     /locus_tag="AC159P_11520"
     CDS             complement(1324..2310)
                     /locus_tag="AC159P_11520"
                     /inference="COORDINATES: protein motif:HMM:NF024881.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HIR3696120.1"
                     /translation="MKCEACLPLLEEYADGELDAGAAARVEAHLEDCVACARAHRKLE
                     AERAVFLAYECDAAPAARFWDDVLSKIEADANAPASSVRPASLPARSRLAAALGVFAA
                     PRLSPALTAALLLVAVASTVALMKYAAPREALPARETAEATAPRAADEGAASSSSSPS
                     SNRGGAVGSVGPARGELERAAAEIEGGEREGNKAGKNVAREGRSDNAASRQRGAERFA
                     RVNRAGGDEASEARRAAASRQEFAPDRLVREAEQKYLAAIRLLSRDVSSRRARLDEET
                     RARFEQTLAAVDRTIADTRRAAREHPRDPVAVQYMLTAYAKKVEVLREMTRY"
     gene            complement(2300..2875)
                     /locus_tag="AC159P_11525"
     CDS             complement(2300..2875)
                     /locus_tag="AC159P_11525"
                     /inference="COORDINATES: protein motif:HMM:NF019265.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR3696121.1"
                     /translation="METPAELIARARRGDGEAFRLLFERYTRPVISFIFYTVGRRALA
                     EELAQETFLRAYKNLSVLRDEAKLSTWLFGIARNVVREELRRRARGGEPQGCVELDDP
                     AAATLRDEKPSPSSRLFGKELGAAVGAALSALDEDKRTVFVLKFYHQRSYEEIAAVTG
                     FSPAKVRNDLHRARAEMRRRLKPYVGGDDEV"
     gene            complement(3126..3992)
                     /locus_tag="AC159P_11530"
     CDS             complement(3126..3992)
                     /locus_tag="AC159P_11530"
                     /inference="COORDINATES: protein motif:HMM:NF024816.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3696122.1"
                     /translation="MRPARFVWAALLVPFFVHASFAQPPRTDGARSGDDDRRGAEHYR
                     AGRFEEAVESFARAVRARPTDANAVNNLGMAYSALGRREEAEAALKRAVGLKPDFAAA
                     HYNLGSVYYEAGRREEAAAAFAEAVRLKPEHANAQHNLGVVRLELGRYAEAVESLKEA
                     VRLAPRWAQANYMLALAHYGSGRRAEAVEFLREAVRLKPAYAAAHNSLGVAHYHAGRR
                     AEALGSFRAAVRTNPSYAAARYNLGVAGLALSERDVAFEQYRALKSLDETLAARLYGG
                     LYKNMLLDVTNK"
     gene            complement(4273..4346)
                     /locus_tag="AC159P_11535"
     tRNA            complement(4273..4346)
                     /locus_tag="AC159P_11535"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4310..4312),aa:Glu,seq:ctc)
     gene            4525..5670
                     /locus_tag="AC159P_11540"
     CDS             4525..5670
                     /locus_tag="AC159P_11540"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015214884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HIR3696123.1"
                     /translation="MRIALLRETQPGEARVALVPESVKKLVALKAEVSVEAGAGREAG
                     ASDEDYRSAGAEVSEGRGALLSTADVLAAVNRPDESDIARMKRGAALVGFLRPLDEPE
                     ELRPLAAQGLTSFAMELVPRITRAQAMDALSSMATVAGYKAVLVAAGELPRMFPLLMT
                     AAGTVPPARVLVLGAGVAGLQAIATARRLGAVVEAYDVRAAAGEQVRSLGAKFLEVDL
                     GGIETEDKGGYAKELTPEALERGRQLIAEHARTADVIVTTAQVPGRPAPLLVTAEAVA
                     GMRRGSVIVDLAGASGGNCAASRPGETTDASGVRVMCPLNLPATVPVHASQLYSRNVT
                     AFLSLVVKDGQLDIDLKDDVVGPSCVTHAGEFVNERVAAALGALASR"
     gene            5715..6041
                     /locus_tag="AC159P_11545"
     CDS             5715..6041
                     /locus_tag="AC159P_11545"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006617139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha part 2"
                     /protein_id="HIR3696124.1"
                     /translation="MKPETLIAALYVFALAAFLGYQVISRVPPLLHTPLMSATNAISG
                     ISLVGSIVIAGTEGHDAVHLFGYGLSTLLGFVAVTCATINVVGGFMITDRMLRMFKRK
                     ELPAKK"
     gene            6140..>7234
                     /locus_tag="AC159P_11550"
     CDS             6140..>7234
                     /locus_tag="AC159P_11550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015934359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HIR3696125.1"
                     /translation="MARFYFIEFTYLAAGILFILGLKGLSHPESAKRGMHLAEFGMLL
                     AIVGTLLHFDIVSYELIIAGLVVGGLVGGAMAYWVPMTAMPQRTALSHAFGAFAAALV
                     GVAEYMRHGASLGDVKMAAINFEVMLGSLTTTGSLVAFAKLQGLTRGTPMTFRGQNVF
                     NMALLTAVLGLFVYLIIFPGSATAFYTMMGLAFVFGVMLVLPIGAADMPVVMSLLNSY
                     AGLAAAATGFVLSNNVLIIAGTLDGFSGFILSILMCRAMNRSIWNVLFGAFGSAAEGA
                     GEGGEQGEMREVNVEDIAVQMAYAKQVVFVPGYGLATAQAQHVVRELAAALEERGVGV
                     KYAIHPVAGRMPGHMNVLLAEANVPYSQLYE"
BASE COUNT         1040 a         2501 c         2479 g         1214 t
ORIGIN      
        1 atctttcttc aggctgttgc tttgactgcc cgctgcctgc tgcctgctgc ctgctgcccg
       61 ctgcctactg cttgcattcc gcggtcgtgc cgggggcggc tctgacgagt ttcaccctgc
      121 cgtcgaagga gtcgagcgtg acctgcgcct gtccgttgcc gtagcgcccg aggacgcggt
      181 tgttgacgac gttcgggccg gacttcaacg tcttcttcag cgagaagccg tccacgatct
      241 ggccgcggta ggaggagagc agcgcggtga agccgggcgg gttgggcgcg agccacatct
      301 cgacggcgcc cgacatcgac ttcaagtggt aacgcccgtc ggcgcgcagc gcgccctcga
      361 agcggatttc gccgctgacg gtcgtcgcgt ccgcgtcgcc gccgacccgc gcgagcgcga
      421 ccgagccgct cgtgttgttg acgttgacgc gccccgtcac gcactcgacc tcgatctcgc
      481 cgctcagcga gagcacgcgc acgtcgccgc gcacgtggcg cacgttgaca tcgccgctct
      541 cggtcatcac gtccaccagc ccgcccgcgc cctcgacctc cacctcgccg ctcgccgtgc
      601 gcaccgcgac cgcgccgacg cggcgcagcc tgatcgcgct ccgcctgccg ttgacggcga
      661 ccggcgtctc gacgccctcg atctcgacct cgctgcggtt cacctcgatc agctcgatct
      721 ccgcgtagcg cggcagcttc acttcgagat gcacctcgct tggccgctgc tcgaagagga
      781 actcgaacac cttctccctg gcttcggcca agaccgactc gttagtccgc gcggctgcgg
      841 gcggcccctg tgatttcgcc tctacgccgc cctgcgccgg cgaagcgggc gtggcggcgg
      901 gcgcgggcgg cacggacttc gggccttcgt ccggcgggtt cggcagccgc ttctccggcg
      961 tcaggtagtc tgccgtcaga gagacacgct gccccgacgg gtctcgcccc gaataggcgc
     1021 gcacgtactc cctgccgcgc tcgctcgtcg ccacggcctc gacgtagtcc ctgtcccacc
     1081 cgctgacgac gatgcgaccg gtcgtccggt tgtccacctc gaccttggcg ccgcgctcca
     1141 gcctgacgcg caccgtgcgc gcctcttcgt cgcgctcgac gggcggcgcg tcgccttgcg
     1201 ccggcgccgg gacgtggcac gcgagggcgg cgagcagcgc gaagatgatc gtgcgaatct
     1261 ttgtcatacg aagaccgggt taaagaaggg cgccgttcga gtcgaacctt tcggcgggcc
     1321 gcctcaatag cgcgtcatct cgcggagcac ctccaccttc ttggcgtagg ccgtcagcat
     1381 gtactgcacg gcgacggggt cgcgcgggtg ttcgcgcgcg gcgcggcgcg tgtccgcgat
     1441 ggtgcggtcg acggcggcga gcgtttgctc gaagcgcgcg cgcgtctctt cgtcgagccg
     1501 cgcgcggcgg ctgctcacgt cgcgcgagag cagccggatg gccgccaggt atttctgctc
     1561 ggcctcgcgg acgagccggt cgggcgcgaa ctcttggcga gaggccgccg cccgccgcgc
     1621 ctcgctcgct tcgtcgccgc ccgccctgtt cacgcgcgcg aatctctccg cgcccctctg
     1681 tcttgaggcg gcgttatcgc tccgtccctc cctcgctacg ttcttcccag ccttgttacc
     1741 ctcacgttcg cccccttcaa tttcggcagc ggctctctca agctcgcccc gcgcagggcc
     1801 gacgctcccg acggccccgc cccgattcga agaaggcgaa gacgaagaag acgctgcccc
     1861 ttcgtccgcc gcgcgcggcg cggtcgcttc ggcggtctcg cgcgccggca gcgcctcgcg
     1921 cggcgccgcg tacttcatca gcgccacggt cgaggcgacg gcgacgagca ggagcgcggc
     1981 ggtgagcgcg gggctgaggc gcggcgcggc gaacacgccg agcgccgccg cgagccgcga
     2041 gcgcgcaggc agggaagccg gtcgcacgga cgacgcgggc gcgttcgcgt cggcttcgat
     2101 cttcgagagc acgtcgtccc agaacctcgc ggcgggcgcg gcgtcgcact cgtaggcgag
     2161 gaagacggcg cgctccgctt cgagcttccg gtgtgcgcgc gcgcacgcga cgcagtcttc
     2221 cagatgcgcc tccacgcgcg ccgccgcgcc cgcgtccagc tcgccgtccg cgtactcttc
     2281 gagcagcggc aggcacgctt cacacttcat cgtctccccc cacgtaaggc ttcaggcggc
     2341 ggcgcatctc cgcgcgcgcg cggtgcaggt cgttccgcac cttcgcgggc gagaagcccg
     2401 tcaccgcggc gatctcctcg tagctccgct ggtgatagaa cttgaggacg aagaccgtcc
     2461 gcttgtcctc gtcgagcgcg gagagggccg cgcccaccgc cgcgcccagc tccttgccga
     2521 acaggcgcga agagggcgag ggcttctcgt cgcgcaaggt ggccgccgcc gggtcgtcca
     2581 gctcgacaca gccttgcggc tccccgccgc gcgcgcgccg ccgcagctcc tcgcgcacga
     2641 cgttgcgcgc gatgccgaag agccacgtcg agagcttcgc ctcgtcgcgc agcacgctca
     2701 agtttttgta ggcgcgcagg aaggtctcct gcgccagctc ctcggcgagc gcgcgccgcc
     2761 cgaccgtgta gaagatgaag ctgatgacgg ggcgcgtgta gcgctcgaag agcagacgga
     2821 aagcctcccc gtcgccgcgc cgggcgcgcg caatcagctc tgcgggagtc tccatcgaac
     2881 ccttcgcctg tgcgctgccc gactggctcg ccggagtcgc cgccggtaac tctatcgcca
     2941 gccgcctcat tatcgaatca tacgcgcgtt ccgtccctcc gttcacatct ttcggcgcgc
     3001 gccgcctcgg atggcggatt gtggatggcg gatggcggat tgttcatgtt tcggcagtcg
     3061 agcctgagaa cccggcttca agcttgggcg gcacgccaat ccgccatcca ccatccgcaa
     3121 tcggctcact tgttcgtcac gtcgagcagc atgtttttat agaggccgcc gtagagccgc
     3181 gcggccagcg tctcgtccag agacttgagc gcgcggtact gctcgaacgc cacgtcgcgc
     3241 tcgctcaggg ccaagcctgc gacgccgagg ttgtaacgcg ccgccgcgta agaagggttc
     3301 gtgcgcaccg ccgcgcggaa gctcccgagc gcctcggcgc ggcggcccgc gtggtaatgc
     3361 gcgacgccga ggctgttgtg cgccgccgcg taagcaggct tcaggcggac ggcctcgcgc
     3421 agaaactcga ccgcctcggc gcgccgcccc gacccgtagt gtgcgagcgc gagcatgtag
     3481 ttggcctgcg cccaccgcgg cgcgaggcgg acggcttcct tcagcgactc gaccgcctcc
     3541 gcgtaccgac cgagttcgag gcggacgacg ccgaggttgt gctgcgcgtt cgcgtgctcc
     3601 ggcttcagcc gcacggcctc cgcgaacgcg gcggcggcct cctcgcgccg ccccgcctcg
     3661 taatagaccg accccagatt gtagtgcgcc gcggcgaagt cgggcttcag accgacggcg
     3721 cgcttgagcg cggcttccgc ctcttcgcgg cggcccagcg ccgagtaggc catcccgaga
     3781 ttgttgaccg cgttcgcgtc cgtcggcctc gcgcgcacgg cccgcgcgaa agactcgacc
     3841 gcctcctcga agcgccccgc gcggtaatgc tccgcgcccc tccggtcgtc gtcaccgctt
     3901 cgcgccccgt ccgtccgcgg cggctgcgcg aaagaagcgt ggacgaagaa gggaacgagc
     3961 aacgccgccc agacgaaccg tgccggtctc atagacactc ctttcgcgcc gccgctttcg
     4021 cgtgttcacg cgccgcttag tgagtggccg acccggcaag aaaatctcaa caatatttcc
     4081 ccgtacgcgc cgcacatttt ttggacgcgc cgcgctccgc cgaacgaagc cgtcgtgcct
     4141 gcgacaacga accgttatgc ggcgatgatg acgcgcggct cgtcctgcgt gcgccttctg
     4201 cgttgagggg aattctagtg agtgtgacaa acgaaaaggg ccaccgggaa ggtggccctt
     4261 attctttcag agtagcgcat acgggactcg aacccgtggt ctccgccttg agagggcggc
     4321 gtgttagccg actacaccaa tgcgcctcga ccgcgaagga attataagtt tcgacgtgcg
     4381 gagttggcaa gcggtcgcag gcaccggctc cgtgccggag gccgaaaacg tgattgctat
     4441 ttcgccgatt tgaattaaac tcggcgggca ttcacaatac agctttcatc acaccgggac
     4501 ggtgcaatca gcgaggagaa gaggatgcgg atcgcgttgc tgcgggagac acagccgggg
     4561 gaggcgcgcg tcgcgctggt gccggagtcc gtgaagaagc tggtcgcgct gaaggcggag
     4621 gtctcggtgg aggcgggcgc ggggcgcgag gcgggcgctt cggacgagga ctaccggagc
     4681 gcgggcgcgg aggtctccga ggggcgcggc gcgctgctct cgacggcgga cgtgctggcc
     4741 gcggtcaacc gccccgacga aagcgacatc gcgcggatga agcgcggcgc ggcgctcgtc
     4801 ggtttcctgc gtccgctcga cgagccggag gagctgaggc cgttggccgc gcaagggttg
     4861 acttcgttcg cgatggaact cgtcccgcgc atcacgcggg cgcaggcgat ggacgcgctc
     4921 tcttcgatgg cgaccgtggc gggctacaag gccgtgctcg tggctgccgg cgagctgccg
     4981 cggatgtttc cgctgctgat gaccgccgcc gggacggtgc ctccggcgcg cgtgctcgtg
     5041 ctgggcgcgg gtgtggcggg cttgcaggcg attgcgacgg cgcggcggct cggcgcggtc
     5101 gtcgaggctt acgacgtgcg tgcggcggcg ggcgagcagg tgcgctcgct cggcgcgaag
     5161 tttctggagg tagacctcgg cggcatcgag accgaggaca agggcggcta cgcgaaagag
     5221 ctgacgcccg aagcgctcga acgcgggcga cagcttatcg ccgagcacgc gcgcacggcg
     5281 gacgtgatcg tcacgaccgc gcaggtgccg ggacggcccg cgcctctgct cgtcaccgca
     5341 gaggcggtcg cagggatgcg ccgcggctcc gtcatcgtag acctcgcggg cgcttcgggc
     5401 ggcaactgcg ccgcgagccg tccgggcgag acgacggacg caagcggcgt ccgcgtcatg
     5461 tgtccactca acctgcccgc caccgtcccc gtccacgcga gccagctcta ctcgcgcaac
     5521 gtgaccgcgt tcctcagcct cgtcgtcaaa gacggccagc tcgacatcga cctcaaagac
     5581 gacgtggtcg gcccctcctg cgtcacgcac gcgggcgagt tcgtcaacga gcgcgtggcc
     5641 gccgccctcg gggcgttggc gagccgttga agcgtcgggc gattcaccaa gtcgtcgaag
     5701 gaggtgccct ttaagtgaag ccggagacac tcatcgccgc gttgtacgtg ttcgcgctgg
     5761 cggcgttttt ggggtatcag gtgatctcgc gcgtgccgcc gctgttgcac acgcctctca
     5821 tgtcggcgac gaacgcgatc tcgggcatct cgctcgtggg gtcgattgtc atcgccggga
     5881 cggaggggca cgacgcggtg catctgttcg gatacggcct gagcacgctg ctcggcttcg
     5941 tcgccgtcac ctgcgcgacc atcaacgtcg tcggcggctt catgatcaca gaccgcatgc
     6001 tccgcatgtt caagcgcaag gagctgccgg ccaagaaata gtggtcagtg gtcggtggcc
     6061 agtgaaagac aagagcaaga ctgctccctc ttcttcgtgc ttttcactga ccaccgacca
     6121 ctgaccactg ataaaaacga tggcgcgatt ctacttcatc gagttcacgt acctcgcggc
     6181 gggcatcctg ttcatcctgg ggttgaaggg gttgagccac ccggagtcgg cgaagcgcgg
     6241 gatgcacctg gcggagttcg ggatgctgct ggcgattgtc gggacgctgc tgcacttcga
     6301 catcgtcagc tacgaactca tcatcgccgg attagtggtc ggcgggctgg tcggcggggc
     6361 gatggcctac tgggtgccga tgacggcgat gccgcaacgc acggcgctct cgcacgcgtt
     6421 cggggcattc gcggcggcgc tcgtgggcgt ggccgagtac atgcggcacg gggcgagcct
     6481 cggcgatgtg aagatggcgg cgatcaactt cgaggtgatg ctcggctctc tgacgacgac
     6541 ggggagcctc gtcgcgttcg ccaagttgca gggcctgacg cgcgggacgc cgatgacgtt
     6601 ccgcgggcag aacgtcttca acatggcgct gctgacggcg gtgctcggcc tcttcgtcta
     6661 tctcatcatc ttccccggct cggcgacggc cttctacacg atgatgggac tggcgttcgt
     6721 gttcggcgtg atgctggtct tgcccatcgg cgcggcggac atgccggtgg tgatgtcgct
     6781 cttgaactct tacgcggggc tggcggcggc ggccacgggg ttcgtgctct cgaacaacgt
     6841 gctcatcatc gcgggcacgc tcgacggctt ctcgggcttc atcctgtcga tcctgatgtg
     6901 ccgcgcgatg aaccgctcga tctggaacgt gctgttcggc gcgttcggga gcgcggcgga
     6961 aggcgcgggc gagggcggtg agcagggcga gatgcgcgag gtcaacgtcg aggacatcgc
     7021 cgtgcagatg gcctacgcca agcaggtcgt cttcgtgccc ggctacgggc tggcgacggc
     7081 gcaggcgcag cacgtcgtgc gggagctggc tgccgcgctc gaagagcgcg gcgtcggcgt
     7141 caagtacgcc atccacccgg tcgcgggccg catgcccgga cacatgaacg tgctgctcgc
     7201 cgaagccaac gtcccctact cgcagctcta cgag
//
LOCUS       DBNGOH010000594         2815 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_638011, whole genome shotgun sequence.
ACCESSION   DBNGOH010000594 DBNGOH010000000
VERSION     DBNGOH010000594.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2815)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2815)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2815
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_638011"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(143..994)
                     /locus_tag="AC159P_11555"
     CDS             complement(143..994)
                     /locus_tag="AC159P_11555"
                     /inference="COORDINATES: protein motif:HMM:NF021501.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2225 domain-containing protein"
                     /protein_id="HIR3696126.1"
                     /translation="MRQTLSRSALAAALVLALCGAHAPAHPAHAPAHPPHALAITWFP
                     QEFECPVCRTKNVFLVVGSYGSYIYQYESKFQLIFWPYTDSPTVYSCKKCRLSAFMGD
                     FEQTPKEKHAEILKRLEGVKLEPRKDGESATKYYKDAVYLDIPLSERLLAAQRVYEVM
                     GRDDEFWCHFQRVLGHRYEAEGKRPEADAARREALRLAEKLLGDRGRAGERKEFLYIS
                     GAMRHFLKDDAGALKAFREAMPLKYENKELDKEKNGNYDEFLSDLLRQYVEKIERPAP
                     KEKPKAD"
     gene            complement(1126..>2815)
                     /locus_tag="AC159P_11560"
     CDS             complement(1126..>2815)
                     /locus_tag="AC159P_11560"
                     /inference="COORDINATES: protein motif:HMM:NF025885.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HIR3696127.1"
                     /translation="HMTRTVRHWDDEEVSFLQSIARQIGVGYQYAQLYTGVQREASRT
                     RALLEIANILNARSDFGEVTSHVLDRAIALVGADYCALGVVEPSGKAINLAAFKAAPR
                     AETSGVRELIEAHGQSIPISAIPPLVELLAESKTLRVLDSTLPEPIRLAFNATLGGHA
                     ALVAPVRIGAQTFGLLGFVWSEPRETFDEHEVALVEGIAVQIGTALERDQLSAEVMRL
                     RNVLHERHAEDRIIGQAPSITRAIELALNVADTATSVLIQGESGTGKELLANLIHYNS
                     RRKDKPYVKLNCGAIPETLLESELFGHERGAFTDARQRRRGRFEEADGGTLFLDEIGE
                     MSLAAQVRLLRVLQDGEFARVGGNEILKGDVRVVAASNVDLARAVEQGTFRRDLYYRL
                     SVFPIELPPLRERRADIHPLVIHFLEHHKQKTGRFVSGISREALDALVGYDWPGNVRE
                     LENAIERAVIIASGRQIELDDLPEAVSGTVREERARTRRERFRAASEGRDITLEVSVP
                     ATIDEIERRAIEATLDYTGGDKTRAARALGIGRKTLYRKLRHYDGAGEGPPDDE"
BASE COUNT          406 a          883 c         1049 g          477 t
ORIGIN      
        1 atcccgcgcg actataacaa aagcgcacaa aacggcgcaa gaatatcgcc cgccggagcg
       61 aggcagtccg cgggcaggag agcaccggcg ccggcggcgc gggcgaagcg cggcggggcg
      121 gagaggaagt ccgcgcgcgg cgtcaatcgg ccttcggctt ctccttcggg gcggggcgct
      181 cgatcttctc gacgtactgg cgcagcaggt cggagaggaa ctcgtcgtag ttgccgttct
      241 tctccttgtc gagttctttg ttctcgtact tcagcggcat cgcctcgcgg aaagctttga
      301 gcgcgcccgc gtcgtccttc aggaagtggc gcatcgcgcc ggagatgtag aggaactctt
      361 tgcgctcgcc cgcgcggcca cggtcgccga gcagcttctc cgcgaggcgc agcgcctcgc
      421 gccgcgcggc gtcggcctcg ggccgcttgc cctccgcctc gtagcgatgg ccgagcacgc
      481 gctggaagtg gcaccagaac tcgtcgtcgc ggcccatcac ttcgtagact ctctgcgccg
      541 cgagcagccg ctccgacaaa gggatgtcga ggtagaccgc atccttgtaa tacttggtcg
      601 cgctctcgcc gtccttgcgc ggctcaagct tgacgccttc gaggcgcttc agaatctccg
      661 cgtgcttctc cttcggcgtc tgctcgaagt cccccatgaa agcgctcagg cggcatttct
      721 tacaactgta gacggtcggg ctgtcggtgt agggccagaa gatgagctgg aatttcgact
      781 cgtactgata gatgtagctg ccatagctgc cgacgacgag gaagacgttc ttcgtccggc
      841 agacggggca ctcgaactcc tgcgggaacc atgtgatggc caaagcgtgc ggcgggtggg
      901 ccggggcgtg cgcgggatgg gcgggggcgt gcgcgccgca gagcgcgagg acaagcgccg
      961 cggcgagggc ggagcggctg agagtctggc gcatgattct ttcctcctgg cgttcggggc
     1021 gagggcgggt gcctggcact cgggagactt ggatgcggag acttagacgc gcggcgcggc
     1081 gtcaggttcc ggatgttgtc gataaagggt cgcgtggacg ccaagtcact cgtcgtcggg
     1141 cggcccttcg cccgcgccgt cgtagtggcg gagtttgcgg tagagcgtct tgcgaccgat
     1201 gccgagggcg cgggcggcgc gcgtcttgtc gccgccggtg tagtcgagcg tggcctcgat
     1261 ggcgcggcgc tcgatctcgt cgatggtcgc gggcacgctg acttcgagcg tgatgtcgcg
     1321 gccttcgctg gcggcgcgga agcgttcgcg gcgcgtgcgt gcgcgctcct cgcgcaccgt
     1381 gccgctgacg gcctcgggca gatcgtcgag ttcgatctgt cttccggagg cgatgatgac
     1441 ggcgcgctcg atggcgttct ccagctcgcg gacgttgccc ggccagtcgt agccgacgag
     1501 cgcgtccagc gcctcgcgcg agatgccgga gacgaagcgc ccggtcttct gcttgtggtg
     1561 ttcgaggaag tggatgacga gcgggtggat gtcggcgcgg cgctcgcgca aggggggcag
     1621 ctcgatgggg aagaccgaga ggcggtagta gaggtcgcgg cggaaggtgc cctgctcgac
     1681 ggcgcgcgcc agatcgacgt tcgacgcggc gacgacgcgc acgtcgccct tgaggatttc
     1741 gttgccgccg acgcgcgcga actcgccgtc ctgaagcacg cgcagcagac gcacctgcgc
     1801 ggcgagactc atctcgccga tctcgtcgag gaagagcgtg ccgccgtcgg cctcttcgaa
     1861 gcgaccgcgg cggcgctggc gcgcgtcggt gaaggcgccg cgctcgtggc cgaacagctc
     1921 cgattcgagc agcgtctcgg ggatcgcgcc gcagttgagc ttgacgtacg gcttgtcctt
     1981 gcgccgcgag ttgtagtgga tgaggttggc cagcagctcc ttgcccgtgc ccgactcgcc
     2041 ttgaatcagg acggaggtcg ccgtgtcggc gacgttcagg gccagctcga tggcgcgggt
     2101 gatcgagggc gcctgtccga tgatgcggtc ttcggcgtgg cgctcgtgga gcacgttgcg
     2161 caggcgcatg acctcggcgg agagctggtc gcgctcaagc gcggtgccga tctgcacggc
     2221 gatgccttcg accagcgcga cctcgtgctc gtcgaaggtc tcgcgcggct cgctccacac
     2281 gaagcccagc agcccgaagg tctgcgcgcc gatgcgcacc ggcgcgacga gcgcggcgtg
     2341 cccgcccaag gtcgcgttga aggcgaggcg gatcggctcg ggcagggtcg agtcgagcac
     2401 gcgcagcgtc ttcgactcgg ccagcagctc gacgagcggg gggatggccg agatggggat
     2461 cgattgcccg tgcgcttcga tcagctcgcg cacgcccgaa gtctcggcgc gcggcgcggc
     2521 cttgaaggcg gcgaggttga tggccttgcc cgacggctcg acgacgccga gggcgcagta
     2581 gtcggcgccg acgagcgcga tggcccgatc cagcacgtgc gaggtgacct cgccgaagtc
     2641 ggagcgcgcg ttgaggatgt tggcgatctc gaggagcgcg cgcgtgcggc tcgcctcgcg
     2701 ctggacgccc gtgtagagct gcgcgtactg gtagccgacc ccgatctgcc gcgcgatgga
     2761 ctggaggaag gagacctcct cgtcgtccca gtggcgcacc gtgcgcgtca tgtgg
//
LOCUS       DBNGOH010000595         1846 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3754013, whole genome shotgun sequence.
ACCESSION   DBNGOH010000595 DBNGOH010000000
VERSION     DBNGOH010000595.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1846)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1846)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1846
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3754013"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..271)
                     /locus_tag="AC159P_11565"
     CDS             complement(<1..271)
                     /locus_tag="AC159P_11565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696128.1"
                     /translation="MKARLFLLLSFAVVLCAPHAHAQRRRHAAAPQSATTARAVADAP
                     TRRAGQTLPIRRVILYSNGVAYIERRGYVTGHAEVDLSFKQSQVDD"
     gene            complement(586..1845)
                     /gene="preA"
                     /locus_tag="AC159P_11570"
     CDS             complement(586..1845)
                     /gene="preA"
                     /locus_tag="AC159P_11570"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015236061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017113 - dihydropyrimidine dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0006210 - thymine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HIR3696129.1"
                     /translation="MSVNFAGIKSPNPFWLASAPPTNMGSMVERAFDAGWGGAVWKTL
                     GEPIRNVCSRLAGLDHGPQRMIGLNNIELITDRPLDVNLKEIREVKKKYPDHALIISL
                     MVESRREAWHEIMKQVQDTGADGVELNFGCPHGMSERGMGAAMGQVPEYTCMVTEWVK
                     EVATIPVIVKLTPNVTSITPPARAAVKGHADALSLINTINSVMGVNLDTLVPHPNVNG
                     MAAHGGYCGPAVKPIALNMVSELARDAEVRVPISGIGGISTWRDAAEFLLLGATTVQV
                     CTAVMHYGYRIVEDMIDGLSNYLDEKGFASVHDIIGRSVASVTDWGNLDLNYKTVARI
                     DYSKCIQCNLCHVACEDGAHQCIPLLEVEGRRLPVVDEAECVGCNLCYLVCPSPGCIT
                     MARLDDGSTPLTWRELTSREPAPTSGD"
BASE COUNT          304 a          568 c          642 g          332 t
ORIGIN      
        1 cgtcgtccac ctgcgactgc ttgaaggaga ggtcgacctc ggcatggccc gtgacgtagc
       61 cgcgccgctc gatgtaggcg acgccgttgc tgtagaggat gacgcggcgg atgggcaagg
      121 tctgccccgc gcgccgcgtg ggcgcgtcgg cgaccgcgcg ggccgtcgtc gcactctggg
      181 gcgcggcggc gtggcggcgg cgttgcgcgt gtgcgtgcgg cgcgcacagt acgaccgcga
      241 acgagagcag caggaagagt ctggctttca tgtgggcctc cgaaggtaga gcttgacagg
      301 tgtcgtgctt tgacggcgca gggagacaga cggcggcgcc agccttagtg acggcagtca
      361 ccggcacacg ttaagaacga ctggcgcgtt gctttcttga tgcggtgaag aggggaaaag
      421 gttggcgcgc gcgtatcgac tcgaacgcgc ggcgaagaag atttcaccac gaagacacga
      481 aggcacgaag aaagaaacga gaaggcgaaa acattccggc tctcttttct ttgtgtggct
      541 ccgtgccttc gtgtctttgt ggttaatgtc ttccgctgtc ggaaatcagt cgcccgaagt
      601 aggcgccggt tcgcggctcg tcagctcgcg ccacgtgagg ggcgtgctgc cgtcgtcgag
      661 gcgcgccatc gtgatgcagc cgggcgaggg gcacaccaag tagcagaggt tgcagccaac
      721 gcactcggcc tcgtccacga cgggcaggcg ccggccttcc acttcgagga gcgggatgca
      781 ctggtgcgcg ccgtcctcgc aggcgacgtg gcagaggttg cactggatgc acttggagta
      841 gtcgatgcgc gcgaccgtct tgtagttgag gtcgaggttg ccccagtccg tgacgctcgc
      901 gacgctgcga ccgatgatgt cgtgcaccga ggcgaagccc ttctcgtcca gataattcga
      961 gaggccgtcg atcatgtcct cgacgatgcg gtagccgtag tgcatgacgg cggtgcagac
     1021 ctgcacggtg gtcgcgccga gcaggaggaa ctccgccgcg tcgcgccacg tcgagatgcc
     1081 gccgatgccc gagatgggca cgcgcacctc ggcgtcgcgc gccagctccg agaccatgtt
     1141 gagcgcgatg ggcttgaccg ccgggccgca gtagccgccg tgcgcggcca tgccgttgac
     1201 gttcgggtgt ggcaccagcg tgtcgaggtt gacgcccatc acggagttga tcgtgttgat
     1261 gagcgagagc gcgtcggcgt gtcccttgac ggcggcgcgc gcgggggggg tgatgctcgt
     1321 cacgttgggc gtgagcttga cgatgaccgg gatggtcgcc acctccttga cccactccgt
     1381 caccatgcac gtgtactccg gcacctgccc catcgccgcg cccatgccgc gctcgctcat
     1441 cccgtggggg cagccgaagt tcagctccac gccgtcggcg cccgtgtcct gcacctgctt
     1501 catgatctcg tgccacgcct ctctgcggga ctcgaccatc aaagagatga tgagcgcgtg
     1561 gtccgggtac ttcttcttga cctctcgaat ctctttcagg ttaacgtcga gcgggcgatc
     1621 cgtaatcagc tcgatgttat tgaggccgat catgcgctgc ggcccgtggt cgaggcccgc
     1681 gaggcgcgaa cagacgttgc ggatcggctc gcccaaagtc ttccacaccg cgccgcccca
     1741 gccggcgtcg aacgcgcgct cgaccatcga acccatgttc gtcggcgggg ccgaggcgag
     1801 ccagaacggg ttcggcgatt tgatgccggc gaaattaaca ctcagg
//
LOCUS       DBNGOH010000596         2726 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3204654, whole genome shotgun sequence.
ACCESSION   DBNGOH010000596 DBNGOH010000000
VERSION     DBNGOH010000596.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2726)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2726)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2726
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3204654"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..1011)
                     /locus_tag="AC159P_11575"
                     /pseudo
     CDS             complement(1..1011)
                     /locus_tag="AC159P_11575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013014426.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140663 - ATP-dependent FeS chaperone
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
     gene            complement(1110..1763)
                     /locus_tag="AC159P_11580"
     CDS             complement(1110..1763)
                     /locus_tag="AC159P_11580"
                     /inference="COORDINATES: protein motif:HMM:NF019884.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR3696130.1"
                     /translation="MVATFRHALGASREGIVQQLRARGSSGADELAASLGVSKQCVRK
                     HLDVLEREGYVEHAPERGDRGRPAHVFRLTTKAEELFPRRYDLIARSLLRQIGEVWGE
                     RGLNTVFCGCAGETIGSLRPQLEGLKFDARVRRLTELLGEMGYEAEAEKLGDGSYVLT
                     EWNCPQMELAREYRQLCDQELIVYRELLGTEVFRESRIAGGATRCSYRVLRPKGRKP"
     gene            1886..2647
                     /locus_tag="AC159P_11585"
     CDS             1886..2647
                     /locus_tag="AC159P_11585"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017909984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3696131.1"
                     /translation="MAVDPTQRFSTRVENYIKYRPTYPPAIVDLLQRECGLTERAVVA
                     DVGSGTGLLAELFLKNGNRVYGVEPNREMREAGERLLAAYDNFVSIDGKAEATTLDEW
                     SVDFVAAGQAFHWFDVDATRAEFRRILKPGGRVVLVWNERRADASPFLAAYEGLFKTY
                     GTDYERVAHRYTGAEMVQPFFGANEVTLRTFANRQVFDFEGLKGRLLSASYAPEPGHP
                     NFQPMMDELRKVFDQHLTDGAVAIEYDTKVYYGQL"
BASE COUNT          468 a          984 c          826 g          448 t
ORIGIN      
        1 ggcgacgcgg cggaacgcct cggcctgcgg cgacgcgggg tggctcacga cgacgggcac
       61 gcccttgtcc ccgccctcgc gcacctcgat gccgatgggc accgcgccga ggaacggcac
      121 gctgtacgcg tccgccaatc attgcccgcc gccctcgccg aagatgttgt aacgcgtgcc
      181 cgtgtccggc gcgacgaagt agctcatgtt ctccacgacg cccaggaccg gcacgttcac
      241 cgtctcgaac atcctgagcg cgcgccgcac gtccgccagc gccacctcct gcggcgtcgt
      301 caccatcacc gcgccctgca ccggcacgag ctgcgccaag cttagttgca cgtcgcccgt
      361 ccccggcggc atgtccacga tcaggtaatc cagctcgccc cactccacgt cttgcaggaa
      421 ctgccgcacc agactgtgca ggatcggccc gcgcacgatc atcggctcgt cgcccggctt
      481 caagaccgcc atcgacatga gcctgatgcc gtgcgcctcc aaaggcacca gcttgttcac
      541 gttcacgcgc ggctgcatgt gcccgacgcc gagcatctgc ggcacgttcg gcccgtacac
      601 gtccgcgtcc atcagcccga cgcgcgcgcc ctgctgcgcc agcgagacgg cgagattcac
      661 ggcgaccgtg gacttgccga cgccgccctt gcccgaagag accgcgacga tgttgcgcac
      721 gccctcgacc gtcagcttct cgccgaggcc gcgacctttc ggcacctccg cgtccatcgt
      781 caccttgacg gaagtgacgc ccggaagctg cgacaccacc tcgcgcgcct cgccttccaa
      841 ctgctcgcgc accgggcacg ccggagtcgt cagcacgatg cggaaactca cgtcgccgcc
      901 cctgatctcc aaatccttga cgaagcccag ccgcacgatg tccttgtgca agtccgggtc
      961 acgcacctgc gtcagcgcac cgtacacgtc agcttcagta acttgtgcca tatcagtcct
     1021 tcgtgaaccg tgaatcgtca accgtgacaa gagaagagca gtgacgattg atgagaggga
     1081 tgctgtttta actcgacacg gtttccggtt cacggtttac ggcctttggg tctcaaaact
     1141 ctgtacgagc agcgcgtcgc gccgcccgcg atgcgcgact cgcggaagac ttcggtgccg
     1201 agcagctcgc ggtagacgat caactcctga tcgcagagct gccggtactc gcgcgccagc
     1261 tccatctgcg ggcagttcca ctcggtcagc acgtacgagc cgtcgccgag cttctcggcc
     1321 tcggcctcgt agcccatctc gcccaacagc tccgtcagcc gccgcacgcg cgcgtcgaac
     1381 ttcaagcctt cgagctgcgg cctcaggctc ccgatggtct cgcccgcgca cccgcagaag
     1441 accgtgttca gtccgcgctc gccccacacc tcgccgatct gccgcagcag cgagcgggcg
     1501 atgaggtcgt agcggcgcgg gaacagctcc tccgccttcg tcgtcaggcg gaacacgtgc
     1561 gcgggccgcc cgcggtcgcc gcgctccggc gcatgctcga cgtagccctc gcgctccagc
     1621 acgtcgaggt gcttcctcac gcactgcttc gacacgccga gcgacgccgc cagctcgtcg
     1681 gcgcccgaag agccgcgcgc gcgcaactgc tgcacgatcc cttcgcgcga agccccgagc
     1741 gcatgtctga atgtggcgac cacagcaagg atatgctact tttacccctg ataatttgtc
     1801 aaccacacgg ttggtttatt aagcctcgcc ggacgcgccg cgcgcgtctt tcgccgattt
     1861 atcacaatgc gggggtgaaa aattcatggc cgtcgatccg acccaacgct tctcgacgcg
     1921 ggtcgagaac tacatcaagt acaggccgac gtacccgccg gccatcgtcg atctgctgca
     1981 aagggagtgc gggctgacag agcgggcggt cgtcgccgac gtgggttcgg ggacggggct
     2041 gctcgcggag ctgttcctga agaacggcaa ccgcgtctac ggcgtcgagc cgaaccgcga
     2101 gatgcgcgag gcgggcgagc gcctgctcgc ggcttacgac aacttcgtca gcatcgacgg
     2161 caaggcggag gcgacgacgc tggatgaatg gagcgtggac ttcgtcgccg ccggtcaggc
     2221 gttccactgg ttcgacgttg acgcgacgcg cgcggagttc cgccgcatcc tgaagcccgg
     2281 agggcgcgtc gtcctcgtct ggaacgagcg gcgcgccgac gcctcgccgt tcctcgcggc
     2341 ctacgagggg ctgttcaaga cctacggcac cgactacgag cgcgtcgcgc accgctacac
     2401 cggcgccgag atggtgcagc ccttcttcgg cgcgaacgag gtcacgctgc ggacgttcgc
     2461 caaccggcag gtcttcgact tcgaaggtct caagggccgc ctgctctctg cctcctacgc
     2521 gcccgaaccc ggccacccga acttccagcc gatgatggac gagctgcgga aagtcttcga
     2581 ccaacacctg accgacggcg cggtcgccat cgagtacgac acgaaggtct actacgggca
     2641 actctgaaga gggggaaagt gaggccaggg gcttgaggct agaggcttgt ttcttgcaag
     2701 cctctagccg caagcccctg gcctca
//
LOCUS       DBNGOH010000597         6868 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2104254, whole genome shotgun sequence.
ACCESSION   DBNGOH010000597 DBNGOH010000000
VERSION     DBNGOH010000597.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6868)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6868)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6868
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2104254"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(307..1347)
                     /locus_tag="AC159P_11590"
     CDS             complement(307..1347)
                     /locus_tag="AC159P_11590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HIR3696132.1"
                     /translation="MIGNTPLLEISFNFVGRRRVVYAKAEHLNMTGSIKDRMAFHILR
                     CAYRAGAVRPGDLVAEATSGNTGIAFAAIGRALGHEVRIFMPDWMSRERVALIQSLGA
                     RVYPVNREEGGFLGSIRRAEELADREPGVFLPRQFSNAANVEAHEQTTGPEIWWQLHS
                     RSLTPDAFVAGVGTGGTVMGVGRYLRRQKPSVKIYPLEPAESPTLSTGHKTGRHRIQG
                     VSDEFIPAILDLENLDAVVSVHDGDAILMAQKLASRLGLAVGISSGANFLGALKVQDE
                     LGAGAVVATVFPDDNKKYLSTDLLGDEPPRAGYLAPEVELLSYRALKRVCHTCFEPEE
                     LGADLLNPAYRL"
     gene            complement(1501..2088)
                     /locus_tag="AC159P_11595"
     CDS             complement(1501..2088)
                     /locus_tag="AC159P_11595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696133.1"
                     /translation="MTNVLLDFVILAVALVCLYVVGAALYFSARAALRTYTRYRGTRV
                     VVCPETRGYVAVEVDARHAAATAATKGEPELVLSACTRWPERAGCDQACVWQIERAPA
                     DCLVRTMLDEWYAGKSCHFCGQTFGHVRWHEHKPALLTPDHLVVEWREVRPELVPAAL
                     RTHRPVCWNCAVAETFRTGHPELVTDRDSKVAASG"
     gene            complement(2186..2731)
                     /locus_tag="AC159P_11600"
     CDS             complement(2186..2731)
                     /locus_tag="AC159P_11600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696134.1"
                     /translation="MRTIKMLAVAVVLALTGAIYAAGAMQDAAKTKDPAKAPKSCCRK
                     HGGAQTAAHAGMKHEGCCAGGACSKDHTGGHAQTATPKTAAAGEEKAGCCGGSCCCAG
                     GSCGKEHKGAGARAAADGESCCKNGAAFCKGGGENCCKTHKPDAKHAAQQPSVEKNEG
                     CGSCACCAANKADHKAHAGGR"
     gene            complement(3313..3714)
                     /locus_tag="AC159P_11605"
     CDS             complement(3313..3714)
                     /locus_tag="AC159P_11605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696135.1"
                     /translation="MSKIAGDTPANDERDILEVLKAELDYIEKGGYGRSVRTPWKPTS
                     VFQDSLTCINFGYPYRAHPCSECFLDEMTPPERRAEAVPCHHIPLDAEGTTIETVESE
                     EDQTKLEEKVKAWLRAKIREIEDERAASVAG"
     gene            complement(3761..4759)
                     /locus_tag="AC159P_11610"
     CDS             complement(3761..4759)
                     /locus_tag="AC159P_11610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase-like protein"
                     /protein_id="HIR3696136.1"
                     /translation="MDLSTKYMGLRLKNPVVPSASPLSSTLDGIRRMEDAGASAVVMY
                     SLFEEQIYLESVSLSDHLDRGTYSTAEAFSYFPEMRSYGVGPEKYLELLHAAKLAVGV
                     PIIGSLNGTTPGGWIEYARLIEEAGADALELNVYFIPANPYVTGEVVERRYLEILRDV
                     KRTVSIPVAMKLSPYFSSIGDMSRRLVDEGADALVFFNRFYQPDFDLEKLEVEPRLVL
                     SRGHELRLPLCWVAILYGELLTDFAVTSGVHTYEDVLKSLMAGAKVAMMASELLQNGV
                     GRITEILGEVEEWMERNEYESVTQMIGSMSRTNVADPAAFERANYMKMLASYGPLR"
     gene            complement(4840..>6868)
                     /gene="nifJ"
                     /locus_tag="AC159P_11615"
     CDS             complement(4840..>6868)
                     /gene="nifJ"
                     /locus_tag="AC159P_11615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HIR3696137.1"
                     /translation="DAAEEGATFLLNSPHPQEEVWGRLPRPVQQQIIDKRLRFFSINA
                     YDVAKQAGMGTRINTIMQTCFFAISGVLPREEAIAKIKESIKKTYGKRGQAVVRQNYA
                     AVDAALAHLYEIRVPARATGALDRPPTVAPAAPDFVRHVTAEILAGRGDALPVSALPA
                     DGTYPTATAQWEKRNIALEVPAWEPDLCIECGKCVMVCPHSCIRAKVYDEEELAAAPT
                     GFKHMPSKFRELPDKLYTIQVGVEDCTGCQLCVEVCPAKDKSNVSRKALNMREQLPIR
                     EQERANWDFFLKLPDVQKNGAMKFDSVKNVQLLQPLFEFSGACAGCGETPYIRLLTQL
                     FGDRALIANATGCSSIYGGNLPTTPYTVNREGRGPAWSNSLFEDNAEFGLGMRMALDH
                     QSAYARELVHKLRPLIGEALAGGLLNTEQSTDCGVGAQRELVAALKAKLKDNFEPEVR
                     DLLSVADALVSKTVWIVGGDGWAYDIGFGGLDHVIASGQNVNILVLDTEVYSNTGGQA
                     SKATPLGAVAKFAAGGKATAKKDLGMMATKYGNVYVAQVAMGASDTQTVKALLEAEAH
                     DGPSLVIAYSQCIAHGIDMAKGMQHQKLAVDSGYWPLYRFNPARAAEGKNPFQLDSGE
                     PKISLQDYIYTEARYRMLQQSDPAVAKFLLGQAQAAVKTKWEQYKQMAERK"
BASE COUNT         1042 a         2239 c         2301 g         1286 t
ORIGIN      
        1 cgctttccgg ctggccaccg gcctcgtgtt cctggcggcg gataatccaa agcacgcgag
       61 gtcttaggcg acgccgaaac cggagcgctt cgccgatgag tgtagagcgc gagcatttca
      121 acgcggaggg cgcagaggag aaaagatttt ctctgcgccc tccgcgcctt cgcgcgctcc
      181 gcgttgaaat gtgcaggccg gtagactcaa tcgcacgccg cttcaggacg ccgcctcgtc
      241 gccaaaagcc cggcgcgccc tcggaggtga atggctccgg aagatgttgg ccgacactct
      301 cgatggttac aggcggtagg ccggattcaa caaatccgcc ccgagttctt ccggctcgaa
      361 gcaggtatgg cacacgcgct tgagcgcgcg ataactcaac agctcgactt cgggcgcgag
      421 gtagcctgcg cgcggaggct cgtcgcccag caagtctgtg ctcagatatt tcttgttgtc
      481 gtcggggaag acggtggcga cgacggcgcc cgcgccaagc tcgtcctgca ccttgagcgc
      541 gccgaggaag ttcgcgcccg aagagatgcc gacggcgagg ccgaggcgtg aagcgagctt
      601 ctgcgccatc aggatcgcgt cgccgtcgtg gactgagacg accgcgtcga ggttttcgag
      661 gtcgaggatc gcggggatga actcgtcgga gacgccttga atgcggtggc gccccgtctt
      721 gtggccggtc gagagggtcg gcgactcagc cggctccagc gggtagatct tgaccgaggg
      781 cttctgccgg cgcaggtagc gcccgacgcc catcaccgtc ccgcccgtgc cgacgccggc
      841 gacgaaggcg tccggcgtga gggagcgcga gtgaagctgc caccagattt ccggccccgt
      901 cgtctgctcg tgcgcttcga cgttggccgc gttcgagaac tgtcgcggga ggaagacgcc
      961 cggttcccgg tcggccagtt cttccgccct gcggatgctg ccgaggaagc cgccctcttc
     1021 gcggttgacg gggtagacgc gcgcgccgag gctttggatg agcgcgacgc gctcgcggct
     1081 catccagtcg ggcatgaaga tgcggacttc gtggccgagc gcgcgcccga tggcggcgaa
     1141 ggcgatgccg gtgttgccgg aggtcgcctc ggctacaagg tcgccgggcc ggacggcccc
     1201 cgcgcggtag gcgcacctca ggatgtggaa cgccatccga tccttgatgc tgccggtcat
     1261 gttgaggtgc tcggccttgg cgtagaccac gcgccgccgg cccacgaagt tgaaactaat
     1321 ctccagcagg ggcgtgttgc cgatcatgtg cttcagcccg gcgaagcggc tcgatgcggc
     1381 ggcggggacg gcggatgtct ggatcacggc taacctaccc ttcgacgtac ggggcgcaga
     1441 ccgggccgtg acggggcttg actgcgccgg acacgcgact ctccgtcccc gtccccggca
     1501 tcagccggac gccgcgactt tcgagtcgcg gtcggtgacc agctccgggt gccctgtgcg
     1561 gaacgtctcg gcgaccgcgc agttccagca gaccgggcgg tgcgtccgca gggctgcggg
     1621 caccaactcg ggccgcacct ctctccactc gacgacgaga tggtcggggg tgaggagggc
     1681 cggcttgtgt tcgtgccagc gaacatggcc gaacgtctgg ccgcagaagt ggcacgactt
     1741 gccggcgtac cactcgtcga gcatcgtgcg gacgaggcag tctgcgggcg cgcgctcgat
     1801 ctgccagacg cacgcctggt cgcagccggc gcgttcgggc cagcgcgtgc acgcgcttaa
     1861 gacgagttcc ggctcgccct tcgtggcggc ggtcgcggcg gcatgtcgcg cgtcaacctc
     1921 gacggccacg taccccctcg tctccgggca gacgacgacg cgcgtgcccc tgtaacgcgt
     1981 gtacgtgcgt agggccgcgc gcgcggaaaa gtagagcgcc gcgccgacga cgtacagaca
     2041 caccagcgcg acggccagga tcacgaaatc gagtaacaca ttagtcatga cgccacctcc
     2101 ttccggtggg ctgacgacct gatgacgacc cgaaagcgaa ggggcgcggc ctcaagctcg
     2161 cgccccttcg cttcgggttc gcttattagc gaccgccagc gtgtgccttg tggtcggcct
     2221 tgttcgcggc gcagcacgcg cacgagccgc agccctcgtt tttctcgacg gatggctgtt
     2281 gggcggcatg cttcgcatcg ggcttgtggg tcttgcagca gttctcgcca ccgcccttgc
     2341 agaaggccgc accgttcttg cagcaactct cgccgtctgc ggcggcccga gcgccggccc
     2401 ccttgtgttc cttgccgcac gagccgcccg cacagcagca cgaaccgccg cagcaaccgg
     2461 ccttctcttc gcccgccgct gcggtcttcg gcgttgccgt ctgtgcgtgg ccgcccgtgt
     2521 ggtctttact gcacgcgccg cccgcgcaac atccttcgtg cttcatgcct gcgtgcgccg
     2581 cggtctgcgc gccgccgtgt ttccggcaac agctcttcgg ggccttcgcc gggtctttcg
     2641 tcttcgcggc gtcctgcatc gcgcccgcgg cgtagatggc cccggtcagc gcgagcacga
     2701 cggcgaccgc caacatcttg atggttctca tgatcctcat ctcctgatgg ttattgcttg
     2761 ttccaaagac cgacccgcga tgcgcccacg ggcatgcctc tcgaacgcgc gttaaatcag
     2821 gaacacgcaa cagcggaggt gcgccctcgg caagtcgggc gaacggcccc gcgcctcaag
     2881 cggcccgcgc gaaggcatgg gcgcaagggc gtggaaagaa tctccgggcg accgacctgt
     2941 ggagcgctcc ggcagaggcc gaggcttgcg cgccgggtct gacgcctgcc gcgtcttctc
     3001 gcagcacgac gcttcgcggg agccggactc ccgcgccgtc ttcgatgacg tgcgagcgtg
     3061 ggcgggagcg gcctttgtct ccgtcgaacg gtgtgcgtgt gcagggcagg acgcgcgagc
     3121 gaaggccgct gcgcgccgtt gctgttcgtc gtggggctgc gtcgtatcgc ccggttgcgg
     3181 ctgtgccgcg gagacagtcg tcgcgcagca gagagcacac cccgcgccgg ccagccacag
     3241 tccgaagacg actgcgacga ggctgcgcgt gagaccgaac ggttcgcgta gagctacgtt
     3301 catcacaatc tttcagcccg cgacggaggc ggcgcgctcg tcctcgatct ctctgatctt
     3361 cgcgcggagc cacgccttga ccttctcttc gagcttcgtc tggtcttctt ccgactcgac
     3421 cgtttcgatg gtcgtgccct ctgcgtcgag cgggatgtgg tggcagggaa cggcttcggc
     3481 gcggcgctcc ggcggcgtca tctcgtcgag gaagcactcg ctgcacgggt gcgcgcggta
     3541 cggatatccg aagttgatgc aagtcagcga gtcctgaaag accgaagtcg gcttccaggg
     3601 cgtgcgcacg gagcgcccgt agccgccctt ctcgatgtaa tccagttccg ccttcagcac
     3661 ctcaagaatg tcgcgttcat catttgccgg cgtgtcgcct gctatctttg acatggcgtt
     3721 cactcctttc cgaatgtgtg cgctcgtgcg ccgcggtaat tcatcgtagt ggcccgtacg
     3781 aggccagcat cttcatgtag ttcgcgcgtt cgaacgccgc cgggtcggcg acgttcgtgc
     3841 ggctcatgct gccgatcatc tgcgtgaccg attcatactc gttgcgctcc atccattctt
     3901 cgacctcgcc gagaatctcc gtgatgcgcc ccacgccgtt ctgcaacagc tccgaggcca
     3961 tcatcgccac cttggcgccg gccatcagcg acttcagcac gtcttcgtag gtgtgcacgc
     4021 cgctcgtgac ggcgaagtcc gtcaacagct cgccgtagag aatcgccacc cagcagagcg
     4081 gcagccgcag ctcgtgccca cggctcagga cgaggcgcgg ttcgacttcc agcttttcga
     4141 gatcgaagtc gggctggtag aagcggttga agaagacgag cgcgtcggct ccttcgtcta
     4201 cgagccgccg cgacatgtcg ccgatggagc tgaagtaggg gctgagcttc atcgccacgg
     4261 ggatcgagac cgtgcgcttc acgtcgcgca ggatttcgag gtagcgtcgc tcgaccacct
     4321 cgcccgtgac gtaggggttg gccgggatga agtagacgtt gagttcgagc gcgtcggcgc
     4381 ccgcctcctc gatgaggcgc gcgtactcga tccagccgcc cggcgtcgtg ccgttgaggc
     4441 tgccgatgat gggcacgccg accgcgagct tggccgcgtg aagcaactcc agatattttt
     4501 cggggccgac gccgtaagac cgcatttcgg ggaagtagct gaaagcctcg gcggtgctgt
     4561 aagtcccgcg gtcgaggtgg tcgctcaaag acacgctctc aaggtaaatc tgttcctcga
     4621 agagcgagta catcacgacc gcggaagcgc ccgcgtcctc catccgccgg atgccgtcga
     4681 gcgtgctcga cagcggcgac gccgacggca cgaccggatt cttcagccgg aggcccatgt
     4741 actttgtgct caagtccatt tttttaggac tgaggattga ggactgagga ctgagtttaa
     4801 tgacggttct tttactcggt cctcagccct cagtcctcat cacttccttt ctgccatctg
     4861 cttgtactgc tcccacttcg tcttgacggc ggcctgcgcc tgtccgagca ggaactttgc
     4921 gaccgccggg tcgctctgct ggagcatgcg gtagcgcgcc tctgtgtaga tgtagtcctg
     4981 caaggaaatc ttcggctcgc cggagtccaa ctggaacgga ttcttaccct ctgcggcgcg
     5041 cgccgggttg aagcggtaga gcggccagta gccggaatcg acggcgagct tctggtgctg
     5101 catccccttc gccatgtcga tgccgtgcgc gatgcactgg ctgtaggcga tgacgagcga
     5161 ggggccgtcg tgcgcctcgg cttcgagcag cgccttgacg gtctgcgtgt cgctcgcgcc
     5221 catcgccacc tgcgcgacgt agacgttgcc gtacttggtc gccatcatgc cgaggtcttt
     5281 cttcgcggtg gctttgccgc ccgcggcgaa cttcgccacc gcgccgagcg gtgtcgcctt
     5341 cgacgcctga ccgcccgtgt tcgagtagac ctccgtgtcg aggacgagga tgttgacgtt
     5401 ctgccccgaa gcgatgacgt gatcgagtcc gccgaagccg atgtcgtagg cccagccgtc
     5461 gccgccgacg atccagaccg tcttgctcac gagcgcgtcc gcgaccgaca ggaggtcgcg
     5521 cacctccggc tcgaagttgt ccttgagctt cgctttgagc gcggcgacca gctcgcgctg
     5581 cgcgcccacg ccgcagtcgg tggactgctc cgtgttgagc agccctccgg cgagggcctc
     5641 gccgatcaac ggcctgagct tatgcaccag ttcgcgcgcg taagccgact ggtggtcgag
     5701 ggccatccgc atgccgaggc cgaactcggc gttgtcctcg aagagcgaat tcgaccacgc
     5761 cggcccgcgc ccttcgcggt tgacggtgta gggcgtggtc ggcaggttcc cgccgtaaat
     5821 cgaagagcac ccggtcgcgt tggcgatgag ggcgcggtcg ccgaagagtt gcgtcagcag
     5881 gcggatgtag ggcgtctcgc cgcagcccgc gcacgcgccg gagaattcga agagcggttg
     5941 cagaagctgc acgttcttca cgctgtcgaa cttcatggcg ccgttcttct gcacgtcggg
     6001 gagcttgagg aagaagtccc agttggcgcg ctcttgctcg cggatgggga gttgctcacg
     6061 catgttgagg gctttgcgcg agacgttcga cttgtctttg gccgggcaga cctcgacgca
     6121 gagctggcag cccgtgcagt cttcgacgcc gacttgaatc gtgtagagct tgtcgggcag
     6181 ctcgcggaac ttggagggca tgtgcttgaa gcctgtgggc gcggcggcga gttcctcctc
     6241 gtcgtagacc ttcgcgcgga tgcaggagtg cgggcagacc atcacgcact tgccgcactc
     6301 gatgcacagg tcaggctccc acgcgggcac ttcgagcgcg atgttgcgct tctcccattg
     6361 ggccgtggca gtggggtaag tgccgtcggc ggggagggcg ctgaccggca acgcgtcgcc
     6421 gcgcccggcc aggatttcgg cggtcacgtg ccgcacgaag tcgggcgcgg cgggggcaac
     6481 ggtcggcggt cggtcaagcg cgccggtggc ccgcgcggga acccgaatct cgtacaggtg
     6541 tgcgagggcc gcgtccacgg cggcgtagtt ctgccgcact acggcctggc cgcgcttccc
     6601 gtacgtcttc ttgatggatt cttttatctt tgctatcgcc tcctcgcgcg gcagcacgcc
     6661 gctgatggcg aagaagcagg tctgcatgat ggtgttgatg cgcgtgccca ttcccgcctg
     6721 tttcgccacg tcgtaggcgt tgatcgagaa gaagcgcagg cgtttgtcta tgatctgctg
     6781 ctgcacgggg cgcggcaggc gcccccagac ctcctcttgc gggtgcgggc tgttcagcag
     6841 gaacgtcgcg ccctcttccg ccgcgtcg
//
LOCUS       DBNGOH010000598         2039 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1372903, whole genome shotgun sequence.
ACCESSION   DBNGOH010000598 DBNGOH010000000
VERSION     DBNGOH010000598.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2039)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2039)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2039
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1372903"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..957
                     /locus_tag="AC159P_11620"
     CDS             <1..957
                     /locus_tag="AC159P_11620"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HIR3696138.1"
                     /translation="DDESTPALKVEGGVAPAPPSSAAPRDAQKGAPEEVGDDEVVRIT
                     SNLVPLPAFVTDAQGRAVGDLQLRDFELLVDGQPRDIAELTRAETPITMVMLFDNSSS
                     LRAAASRQFEKEAAAGFFRNVMRPVDRAAIVSVSSYPELAQRLTGDVGALVRTIESFA
                     RPEGATALFDSVAYAAEYLRLHRGRKVLVIVSDGTDTISELDFDETLARALALNCQIY
                     AVRTGHSDNTNLRDLAGERRLQEFAAQTGGAVYVPRNRTELRQAFAQIAEDLSRQYVL
                     SYYPTADPPDGRFRKFTLRVSKRPGLQVRTRKGYYTRKAEKP"
     gene            1078..1998
                     /gene="xerC"
                     /locus_tag="AC159P_11625"
     CDS             1078..1998
                     /gene="xerC"
                     /locus_tag="AC159P_11625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HIR3696139.1"
                     /translation="MEHLLAQFLEHLRYERNVSEHTLRNYAIDLAQFLDYLAPADPLT
                     GARREPDVKEIDHITIREWLSELHNQNKKKTSIARKLASLRTFFQFLIREGAVEANPA
                     KLVNTPRLEKRLPNHLSVEDAVRFIETPDTATDLGKRDRAILEFLYATGVRVSELTKL
                     DIRDVDFRGKTVRVQGKRRKERIVPFGDPALHALLDYLTVRGAFLDNAPVAERETQAI
                     FLNYQGTRITTRSVGRMVDKYIRICAGIHDISPHSLRHSFATHLLDSGADLRDIQELL
                     GHARLSTTQIYTHVSMEKLIEVYDKAHPKA"
BASE COUNT          395 a          771 c          581 g          292 t
ORIGIN      
        1 gatgacgaga gcacgcccgc gttgaaggtc gagggcggag tcgcgccagc gccgccttca
       61 tccgccgcgc cgcgcgacgc gcagaagggc gcccccgaag aggtcggcga cgacgaggtc
      121 gtccgcatca cttcgaacct cgtcccgctg cccgccttcg tcaccgacgc gcaaggccgc
      181 gccgtgggcg acctccagtt gagagacttc gagctgctcg tggacgggca gccgcgcgac
      241 atcgcggagc tgacgcgcgc cgagacgccc atcacgatgg tgatgctctt cgacaacagc
      301 tcgagcttgc gcgccgcggc ctcgcggcag ttcgagaagg aggccgccgc cggcttcttc
      361 cggaacgtca tgcgccccgt ggatcgcgcg gccatcgtct cggtctcgtc ctaccccgaa
      421 ctcgcgcagc gtttgacggg cgacgtgggc gcgctcgtcc gcaccatcga gagcttcgcc
      481 cggcccgaag gcgcgaccgc gctcttcgac tcggtcgcct acgccgccga gtacctccgc
      541 ctgcaccgtg gacgcaaggt gctcgtcatc gtctccgacg gcacggacac gatcagcgaa
      601 ctcgacttcg acgagacgct cgcccgcgcc ctcgcgctca actgccaaat ctacgccgtc
      661 cgcaccggcc acagcgacaa cacgaacctg cgcgacctcg ccggcgagcg ccgcctccaa
      721 gaattcgccg cgcagaccgg cggcgccgtc tacgtcccgc gcaaccgcac cgagctgcgc
      781 caagccttcg cccagatcgc cgaagacctc tcgcgccaat acgtcctcag ctactacccc
      841 accgccgacc cgcccgacgg tcgcttccgc aaattcacgc tgcgcgtctc gaagcgcccc
      901 ggcctccaag tccgcacccg caagggctat tacacgcgca aggccgaaaa gccgtgaacc
      961 gtgaaccgta aaccgcgaac cgtgaaaacc gaatcgtggt cggggtgatg agcggcgggc
     1021 tgtgagggcg gaaatttcta tttaccattc accatttacc atttaccatt caggggcatg
     1081 gagcacctac tcgcgcagtt tctcgaacac ctccgctacg agcgcaacgt gagcgagcac
     1141 acgctcagga attacgccat cgacctcgcg cagttcctcg actacctcgc gcccgcagac
     1201 cctttgacgg gcgcgcggcg cgagccggac gtgaaagaga tcgaccacat cacgatccgc
     1261 gagtggctct cggagctgca caaccagaac aagaagaaga cctcgattgc acgcaagctc
     1321 gccagccttc gaaccttctt ccagttcctc atccgcgagg gcgcggtcga agcgaacccg
     1381 gcgaagctcg tcaacacgcc gcgcctggag aagaggctgc ccaaccacct ctcggtcgag
     1441 gacgcggtgc gcttcatcga gacgcccgac accgcgaccg acctcggcaa gcgcgaccgc
     1501 gccatcctcg aattcctcta cgcgacgggc gtgcgcgtct ccgagctgac caaactcgac
     1561 atccgcgacg tggacttccg cggcaagacc gtccgcgtgc agggcaagcg gcgcaaggag
     1621 cgcatcgtgc ccttcggcga ccccgcgctc catgcgctcc tcgattacct gacggtgcgc
     1681 ggcgccttcc tcgacaacgc gcccgtcgcc gagcgcgaga cgcaggccat cttcctcaac
     1741 tatcagggca cgcgcatcac cacgcgctcg gtcgggcgca tggtcgataa gtacatccgc
     1801 atctgcgccg gcatccacga catcagcccg cactccctgc gccacagctt cgccacccac
     1861 ctcctcgaca gcggcgccga cctccgcgac atacaggaac tcctcggcca cgcacgcctc
     1921 tcgacgacgc aaatctacac acatgtctcg atggaaaaac tgatcgaggt ctacgacaag
     1981 gcgcacccca aagcctgaaa ggccgtcaac cgtcaaccgt caaccgtgac aggaggagc
//
LOCUS       DBNGOH010000599         2136 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3388853, whole genome shotgun sequence.
ACCESSION   DBNGOH010000599 DBNGOH010000000
VERSION     DBNGOH010000599.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2136)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2136)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2136
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3388853"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1138)
                     /locus_tag="AC159P_11630"
     CDS             complement(<1..1138)
                     /locus_tag="AC159P_11630"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0016116 - carotenoid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HIR3696140.1"
                     /translation="MARKYDVIVIGGGHNGLVNAAYLARAGRKVLVLERRHVLGGAAV
                     TEEVYPGFKFSVASYVVSLLRPEIIRELDLPRHGLEILPLDGTFTPMPNGDYLWRVND
                     HARTRREIARHSKLDAEAYDEYGKAMVEMGRFVKPIMAMTPPDPTSLSPKGLMDLLFL
                     GRRFRSLSTEDKYNQVQLMTMSAVDFLDRWFETDVLKATMAASGIIGTFLGVRSPGTA
                     YVLLHHYMGEIDGAFRSWGLARGGTGAISESIAGAAREAGADIQKESPIAKIIVKAGQ
                     ARGVALENGDEYEAQVVSSSVDPRLTFMKMVGEEHLPAEFVEDIKRYKFRGSSGKVNM
                     ALDALPDFKAMPGPGAHLRGAVSISPSVEYMERAYDDAKYGRFSR"
     gene            1229..1465
                     /locus_tag="AC159P_11635"
     CDS             1229..1465
                     /locus_tag="AC159P_11635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HIR3696141.1"
                     /translation="MSEQLIQSDPKIMMGKPVVAGTRITVELILEKMAAGETIEQILD
                     AHPRLTRQAVLAALEFAAHALKADVIYPVPEKAA"
     gene            1462..1815
                     /locus_tag="AC159P_11640"
     CDS             1462..1815
                     /locus_tag="AC159P_11640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HIR3696142.1"
                     /translation="MNLLADESVDRQIVERLRQEGHDVLYIAEMEPGITDDVVLGRAN
                     ERGALLVTADKDFGELVFRQGLVNSGVVLLRLAGLSPETRATLLSDVLQDREAELLRA
                     FSVISPGMVRIRRGD"
     gene            1852..>2136
                     /locus_tag="AC159P_11645"
     CDS             1852..>2136
                     /locus_tag="AC159P_11645"
                     /inference="COORDINATES: protein motif:HMM:NF013720.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl transferase family protein"
                     /protein_id="HIR3696143.1"
                     /translation="MPIGTAFHERTLALCESLNYREWSGYYAVGSYEPHFEHEYNAIR
                     NAAALIDVSPLFKYRLTGRDATRLVDRIVTRDMRKVAVGQVIYTPWCDERG"
BASE COUNT          413 a          668 c          668 g          387 t
ORIGIN      
        1 ggcgcgagaa gcgcccgtac ttggcgtcgt cgtaggcgcg ctccatgtac tcgacgctcg
       61 gcgagatgga gaccgcgccg cgcaggtgcg cgcccggccc cggcatcgcc ttgaagtcgg
      121 gcagcgcgtc gagcgccatg ttcactttgc ccgacgagcc gcggaacttg taacgcttga
      181 tgtcttcgac gaactccgcg ggcagatgct cttcgccgac catcttcatg aacgtcaggc
      241 gcggatcgac gctcgaagag acgacctgcg cctcgtactc gtcgccgttc tccagcgcga
      301 cgccgcgcgc ttggcccgcc ttcacgatga tcttcgcaat cggcgactcc ttctggatgt
      361 ccgcgcccgc ctcgcgcgcc gcgccggcga tcgattccga gatggcgccc gtaccgccgc
      421 gcgcgaggcc ccacgagcgg aacgcgccgt cgatctcgcc catgtagtgg tggagcagga
      481 cgtaggccgt gcccggcgag cgcacgccga ggaaggtgcc gatgattccg gaggcggcca
      541 tcgtcgcctt gagcacgtcg gtctcgaacc agcggtcgag aaagtccacc gcgctcatcg
      601 tcatgagctg gacttggtta tatttgtcct cggtcgagag gctgcggaaa cggcgaccga
      661 ggaagagcaa gtccatcagc cccttcggac tcaaagaagt cgggtcgggc ggcgtcatcg
      721 ccatgatcgg cttgacgaag cggcccatct cgaccatcgc cttgccgtac tcgtcgtacg
      781 cctccgcgtc gagcttcgag tggcgcgcga tctcgcgccg cgtccgcgcg tggtcgttca
      841 cgcgccacag gtaatccccg ttcggcatcg gcgtgaacgt cccgtcgagc ggcagaatct
      901 ccaacccgtg ccgcggcaaa tccagctccc ggatgatctc cggcctcagg agcgacacca
      961 cgtacgacgc caccgagaac ttgaaccccg gatacacctc ttccgtcacg gcggccccgc
     1021 cgagcacgtg cctacgctca agcacgagca ccttccggcc cgcgcgcgct aagtaagccg
     1081 cgttcacgag accgttgtgc ccgccgccga tgacgataac gtcgtacttt cgagccatgt
     1141 tttagagggg caatgcttga tgaagcccga ctaggatact tataatcgct ctccgtgaca
     1201 aacacacgat gaaggcgaag gtgcggacat gagtgagcag ttgattcaat cagaccctaa
     1261 aatcatgatg ggcaagcctg tcgtcgcggg cacacgcatc acggtcgaat tgatattgga
     1321 aaagatggct gccggagaaa ccatcgagca aattcttgat gcacacccgc gtttgacgag
     1381 gcaggccgtt ctcgccgcgc tcgaattcgc cgcccacgcg cttaaagccg atgtcatcta
     1441 tccggttccg gaaaaggctg catgaacctt ctggctgatg aaagcgttga ccggcagata
     1501 gttgaacggc tccggcagga aggtcacgac gttctttata tcgcggagat ggagccgggc
     1561 atcacggacg atgttgtcct cggcagggct aatgagagag gcgctttgct agtgacagca
     1621 gacaaggact tcggcgaact cgtcttccgt caagggctgg tcaactccgg ggtcgtactt
     1681 ctacgactgg ctggcttgtc gccggaaacg agagccacat tgttgtctga cgtgttgcaa
     1741 gaccgcgagg ccgaactgct tcgagccttc agcgtgattt caccgggaat ggtgcgaatc
     1801 cgccgcgggg attaattcgc tcgccgtgac aaacacgcgc tagagaatcc catgccaatc
     1861 ggaacagcct ttcacgagag gacgctcgcg ctgtgcgaga gcctgaacta ccgcgagtgg
     1921 tcggggtatt acgccgtcgg ctcgtacgag ccgcacttcg agcacgagta caacgcgatc
     1981 aggaacgcgg cggcgctgat cgacgtgtcg ccgctcttca agtaccgtct gacggggcgg
     2041 gacgcgacgc ggctggtcga taggatcgtg acgcgcgaca tgaggaaggt cgcggtcggg
     2101 caggtgatct acacgccgtg gtgcgacgag cgcggg
//
LOCUS       DBNGOH010000600         4823 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2655116, whole genome shotgun sequence.
ACCESSION   DBNGOH010000600 DBNGOH010000000
VERSION     DBNGOH010000600.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4823)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4823)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4823
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2655116"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(58..654)
                     /locus_tag="AC159P_11650"
     CDS             complement(58..654)
                     /locus_tag="AC159P_11650"
                     /inference="COORDINATES: protein motif:HMM:NF014668.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HIR3696144.1"
                     /translation="MRGRRRKSNARDVREDVEEKRPARPVDAEKARERTLQRAVKLLA
                     AKPRSVEELRERLLEKQWADEAAVEYALDKLKGYGYLDDESFAFGFAASRVRQRPVGR
                     QRLARDLQMKKVSRETADAALDLVFQETPEDRLIAAAIEKRVRLRGRPQTRQETKSLF
                     DHLLRRGFSYDLIIRKVREIGADDVEEDEADDDSVATR"
     gene            849..1796
                     /gene="truB"
                     /locus_tag="AC159P_11655"
     CDS             849..1796
                     /gene="truB"
                     /locus_tag="AC159P_11655"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017592444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HIR3696145.1"
                     /translation="MDGVLIIDKPAGWTSHDVVARVRRVLRERRVGHTGTLDPFATGV
                     LVVLVGRATRLAQFLAGAEKEYEATVRFGYATDTGDLTGARRDDEKYRDRECASLAEE
                     ELESALASLRGEIEQVPPMYSAKKVQGRKLYELARRGEEVERRAVRVRVGVFELMDGP
                     DGAPRLRQSDDGTCDLSTRVVCSAGTYVRVLAESLGERLGTGAHLAALRRTRAGTFGI
                     EDALTLERLQELAEADEVGAKLLSTDAALSHLPAVHLTDDEARKVRHGAAVGDARAPQ
                     GIQDGAHVRLRDAEGSLLAVAVYDATRRLLRPRVMLAAV"
     gene            2133..4067
                     /locus_tag="AC159P_11660"
     CDS             2133..4067
                     /locus_tag="AC159P_11660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrkA family serine protein kinase"
                     /protein_id="HIR3696146.1"
                     /translation="MQEQDGNKFDIGERLESLRRRHEALQWEGTFRDYFELVTQQPRL
                     AQLSHARINDMVHAAGVEKLNEGTRDELVRYNFFAEELFGIEEPIARFVEYFKSAAQR
                     LEVRKRILLLMGPVGGGKSTIVNMLKRGLEEWTRAPEGAVYAIKDCPMHEEPLHLIPH
                     ELRAEIEKHYGIYIEGDLCPQCRYALEHTYGGRHEDVKVHRVLFSEKQRVGIGTFSPS
                     DPKSQDVTELTGSIDLSTIGEVGVESDPRAYRFDGELNIANRGVMEFIEMLKCDEKFL
                     YNLLTLSQEQSIKTGRFAMIYADEVILSHTNENEYVAFAGNRKSEALQDRIILVRVPY
                     NLRVSQEERIYDKLLKQSEILRNVHIAPNTLKVAAMFAVMTRLEEPRRANVDVVKKMK
                     LYDGEDVEGYKSKDVRELKDEAVREGMDGISPRYIINRLSSALVRDGVTCINPIDALR
                     TIKSGFEQHTGINAEQRERYLNLISAARKEYDEMAKTEVQRAFVYSFEEMARTMCNNY
                     LDNVEAYCNKERMKDPITEEEIEPDEQLMRSIEEQIGVSENAKNTFRQEILIRISSYA
                     RKGKQFEYISHERLKEAIEKKIFADLKDVVKITTSAKTPDPDQLRRINEVVDRLIKEH
                     GYCPVCANELLTYVGTLLSR"
     gene            4203..4646
                     /locus_tag="AC159P_11665"
     CDS             4203..4646
                     /locus_tag="AC159P_11665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696147.1"
                     /translation="MGQEDDALESKKEFWRETPESRMLKLLEKLSERVQANEDESRRK
                     ADETQRNMDFIVQQQARFVEDIQKLSEAQARTQGIVERLAVVSLNRFEKVEGELESKM
                     SALIDSHIRLVDTQAQTDERLSTFITTVERLISEGRNGGSDAPEV"
     gene            4651..>4823
                     /locus_tag="AC159P_11670"
     CDS             4651..>4823
                     /locus_tag="AC159P_11670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006446484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YhbH"
                     /protein_id="HIR3696148.1"
                     /translation="MGDAHISGTIQRNDWSLQRKGIIDQERHKERVKEAIRKNLGSIV
                     SNEAIILSDGKRTV"
BASE COUNT          965 a         1529 c         1557 g          772 t
ORIGIN      
        1 actcaacgtc gccttcatcg aaagtcggcg ggccgcggac aagccctgcc cctacagtca
       61 tcgcgtagca accgagtcgt cgtcggcttc gtcctcttca acgtcgtcgg cgccaatctc
      121 ccgcaccttg cggatgatga gatcgtagga gaagccgcgg cgcaggaggt ggtcgaagag
      181 gctcttggtc tcctggcgcg tctgcgggcg accgcggagg cggacgcgct tttcgatggc
      241 ggcggcgatc agccggtcct cgggcgtctc ctgaaagacg aggtcgaggg cggcgtcggc
      301 ggtctcgcgc gagaccttct tcatctgtag gtcgcgcgca aggcgctggc gcccgaccgg
      361 cctctgccgg acgcgcgaag cggcgaaacc gaaggcgaag ctctcgtcgt cgaggtagcc
      421 gtagcccttg agcttgtcga gcgcgtattc gaccgccgcc tcgtccgccc actgcttttc
      481 gagcaggcgc tcgcgcaact cctcgaccga gcgcggcttg gccgccagca gcttcacggc
      541 cctttgcagc gtccgctcgc gcgccttctc cgcgtccacg ggccgcgccg gtcgcttctc
      601 ctccacgtcc tcgcgcacgt cccgcgcgtt gctctttctc cttcggccac gcatcacgaa
      661 taatctaacc cgcccacgcg gcaaagagaa gtgagcagtg gaagcgctgt cggatgttgg
      721 agcgacggtt gacggttgac ggaggcggcg ctgtcatcaa tcattgacgg gggcggcgct
      781 gtcatcgacc gtcaaccgtc ggcgcaacgg caatccgaag tcgaacatcg aaaatccgaa
      841 atattccgat ggacggcgtg ctcatcatcg acaagcccgc ggggtggact tcgcacgacg
      901 tggtggcgcg cgtcaggcgc gtgctgcggg agcggcgcgt gggccacacg gggacgctcg
      961 acccgttcgc gacgggcgtg ctggtcgtgc tcgtcgggcg cgcgacgcgg ctcgcgcagt
     1021 tcctcgcggg cgcggagaag gagtacgagg cgacagtgcg tttcggctac gcgaccgaca
     1081 cgggcgacct gacgggcgcg cggcgcgatg atgaaaagta tcgtgaccgg gaatgcgcgt
     1141 cgctcgccga ggaagagctg gagtcggcgc tcgcgtcctt gcgcggcgag atcgagcagg
     1201 tgccgccgat gtactcggcg aagaaggtgc aggggcggaa actttacgag ttggcgcggc
     1261 gcggcgaaga ggtcgagcgc agggccgtgc gcgtccgcgt cggcgtgttc gaactcatgg
     1321 acggcccgga cggcgcgccg cggctgcggc agagcgacga cgggacgtgc gacctttcga
     1381 cgcgcgtcgt ctgctcggcg ggcacgtacg tgcgcgtcct ggccgaatcg ctcggcgaac
     1441 ggctcggcac gggcgcgcac ctcgccgcct tgcgccgcac gcgcgcgggg actttcggca
     1501 tcgaggacgc gctcacgctc gaacgcttgc aggagcttgc cgaggccgac gaggtcggcg
     1561 cgaaacttct ttcgacggac gcggcgctct cgcatctgcc cgccgtgcat ctaacggatg
     1621 acgaggcgcg caaggtgcga cacggcgccg ccgtcggcga cgcgcgcgcg ccgcaaggga
     1681 tacaggacgg ggcgcacgtg cggctgcgcg acgcggaagg cagcctgctc gccgtcgcgg
     1741 tttacgacgc gacgcgccga ctcttgcgcc cgcgcgtgat gctcgccgcc gtgtaaaaaa
     1801 tagccttgac gagagaggtc aagggctgtg ttataatcat ggtcagcaag ggggacttcg
     1861 aaggagttcg cgcccgcgct cttcccgcag cgcggccagt cttttctctc ccgttgaaaa
     1921 ccgaagatac gttgaccgaa agccggagtc gcttcgaagg cgcggcgtgc actttttacg
     1981 cgcgagtcac cgcagacgcg catcgaagct cgaacgacgg aaaccgaccg cgaagcccgt
     2041 gcttgcaggt ttcgcggccc gcgagaacaa gaagggcgtg accccacgcc gtccacgcgc
     2101 gccacagcct agagaggttt aatacaaagg ctatgcagga gcaggatggc aataaattcg
     2161 acatcggcga gcggctcgaa tccctccgcc gccgccacga ggccctccag tgggagggca
     2221 cgttccgcga ctacttcgag ctggtcacgc agcagccgcg cctcgcgcaa ctctcgcacg
     2281 cgcgcatcaa cgacatggtg cacgccgcag gcgtcgagaa gctgaacgag ggcacgcgcg
     2341 acgaactcgt ccgctacaac ttcttcgccg aggagttgtt cggcatcgag gaacccatcg
     2401 cgcgcttcgt cgagtacttc aagtcggcgg cgcagcgcct cgaagtccgc aagcgcatcc
     2461 tcctcctgat gggccccgtg gggggcggca aatccaccat cgtcaacatg ctcaagcgcg
     2521 gcctcgaaga gtggacgcgc gccccggagg gcgccgtcta cgccatcaag gactgcccga
     2581 tgcacgagga gccgctgcac ctcatcccgc acgagctgcg cgccgagatc gagaagcact
     2641 acggcatcta catcgaaggc gacctctgcc cgcagtgccg ctacgcgctc gaacacacct
     2701 acggcgggcg ccacgaggac gtgaaagtcc accgcgtgct cttctccgaa aagcagcgcg
     2761 tcggcatcgg caccttcagc ccgagtgacc ccaagtcgca ggacgtgacc gagctgaccg
     2821 gctccatcga tctttcgacc atcggcgagg tcggcgtcga gagcgacccg cgcgcttatc
     2881 gtttcgacgg cgagctgaac atcgccaacc gcggcgtgat ggagttcatc gagatgctca
     2941 agtgcgacga gaagtttttg tacaaccttc tcacgctgtc gcaggagcag tcgatcaaga
     3001 cgggccgctt cgccatgatc tacgccgacg aggtcatcct ctcgcacacc aacgagaacg
     3061 agtacgtcgc cttcgccggc aaccgcaagt cggaagcctt gcaggatcgc atcatcctcg
     3121 tccgcgtgcc ctacaacctt cgcgtctcgc aggaagaaag aatctacgac aagctcctca
     3181 agcagtcgga aatcctgcgc aacgtccaca tcgcgccgaa cactttgaag gtggcggcga
     3241 tgttcgccgt catgacgcgc ctggaagagc cgcgccgcgc gaacgtcgat gtcgtcaaga
     3301 agatgaagct ctacgacggc gaggatgtcg aagggtacaa gtcgaaggac gtgcgcgaac
     3361 tcaaggacga ggccgtgcgc gaaggcatgg acggcatcag ccctcgctac atcatcaacc
     3421 gcctgtcgtc ggctttggtg cgcgacggcg tgacgtgcat caacccgatc gacgcgctgc
     3481 gcaccatcaa gagcggcttc gagcagcaca ccggcatcaa cgccgagcag cgcgagcgct
     3541 atctgaacct catctcggcg gcccgcaagg agtacgacga gatggcgaag accgaggtgc
     3601 agcgcgcctt cgtctactcg ttcgaggaga tggcgcgcac catgtgcaac aactacctcg
     3661 acaacgtcga ggcgtactgc aacaaggagc ggatgaaaga cccgatcacc gaggaggaga
     3721 tcgagccgga cgagcagctc atgcgctcca tcgaggagca gatcggcgtc agcgagaacg
     3781 ccaagaacac cttccgccag gagatactga tccgcatctc ttcctacgcg cgcaagggca
     3841 agcagttcga gtacatctcg cacgagcgcc tgaaggaggc catcgagaag aagattttcg
     3901 ccgacctcaa ggacgtggtg aagatcacca cctccgccaa gacgcccgac cccgaccagc
     3961 tccgccgcat caacgaggtc gttgaccgcc tcatcaagga gcacggctac tgccccgtct
     4021 gcgccaacga gctgctgacc tacgtcggca cgctgctctc gcgctgaccc gcgggcattc
     4081 gtcacgcgcc aaacgttccc cgcccgcgtc tctcctggca acgagaggcg cgggcgtgat
     4141 gcttcatcaa ggcggtcgtt tttgtcagtc ggtcgtacct cattgaaagg ggcgaatgaa
     4201 gaatgggtca ggaagacgac gctttagaga gtaagaaaga attctggcgg gagacgcctg
     4261 agtctcgcat gcttaaactt ctcgaaaagt taagcgagcg ggtgcaagcc aacgaagacg
     4321 agtcgaggcg caaagccgat gagacgcaac gtaacatgga ttttattgtt cagcagcagg
     4381 ctcgattcgt cgaggacatt cagaagctga gcgaggcaca ggccagaaca caggggatag
     4441 ttgaacgttt agccgtcgtt tcgctcaacc gttttgagaa ggttgaaggt gagcttgaga
     4501 gcaagatgtc ggcgctgatt gactctcaca tcaggcttgt tgacacccag gctcaaacgg
     4561 atgaaagact aagcacattc attaccaccg tcgagcggtt aatcagcgag gggcggaatg
     4621 gcgggtcgga cgcgccggag gtttgaggcc atgggcgacg cgcacatctc gggcaccatc
     4681 cagcgcaacg actggtcgtt gcagcgcaag ggcatcatcg accaggagcg gcacaaggag
     4741 cgcgtcaagg aggcgatccg caagaacctc ggctccatcg tctcgaacga ggcgatcatc
     4801 ctgtcggacg gcaagcgcac ggt
//
LOCUS       DBNGOH010000601         3141 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1006922, whole genome shotgun sequence.
ACCESSION   DBNGOH010000601 DBNGOH010000000
VERSION     DBNGOH010000601.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3141)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3141)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3141
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1006922"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /locus_tag="AC159P_11675"
     CDS             complement(<1..200)
                     /locus_tag="AC159P_11675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696149.1"
                     /translation="MRSLDKKASAGLCLPPGIVKLSGSIHTTFFDRRIMSTKTLTAFE
                     EFLDRHDEAAWRGALAEMLPHV"
     gene            317..559
                     /locus_tag="AC159P_11680"
     CDS             317..559
                     /locus_tag="AC159P_11680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696150.1"
                     /translation="MSYQEPTTRPTIETLFKIMREVRDEMRAGFVRVNEGFGRIELRL
                     EKVEEQLDRVTGVAHETRADLRELKKDLRDHFPAVK"
     gene            complement(642..992)
                     /locus_tag="AC159P_11685"
     CDS             complement(642..992)
                     /locus_tag="AC159P_11685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017546300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HIR3696151.1"
                     /translation="MSEQNCLFCKIVAGGMPAEKVYEDDGAVAFRDINPQAPVHVLVI
                     PREHIASLADASETDAAKLGQLMLAAARVARQEGLSESGYRTVVNTGAGAGQSVFHIH
                     LHVLGGRTLAWPPG"
     gene            complement(1091..2419)
                     /gene="murA"
                     /locus_tag="AC159P_11690"
     CDS             complement(1091..2419)
                     /gene="murA"
                     /locus_tag="AC159P_11690"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HIR3696152.1"
                     /translation="MYKCRITGGRPLSGRVEISGAKNSALPCMAAALLTGETVTLHNV
                     PYVRDCLTQRRLLEDVGATVLVPDLRTHKITASNIELFEAPYELVKTMRASVLVLGPL
                     LARFGKAKVSLPGGCAIGTRPIDLHLEALKLLGAQVNLEAGDVVARAPQGGRLKGAAV
                     NFNKVTVTGTENVMMAAALAEGETVINNAAREPEISDLAELLNRMGARVRGAGTPAIT
                     IEGVEQLGGAEHTIIPDRIETGTFLIAAAITGGELEVKDCRPEHSATVIAKLREAGVE
                     IEEINQSTLVVRRSPQGLVARDVETEPHPRFPTDMQAQYMALMTQAEGRAVIKETIFE
                     NRFMHASEMQRMGASIQIEGNTAIVEGPTPLAGARVQASDLRASACLVLAGLAAEGET
                     IIDRVYHIDRGYEKIEDKLRSVGARIERLRDSVTAQLPPVEAVVNAEARG"
     gene            complement(2540..>3141)
                     /gene="prmC"
                     /locus_tag="AC159P_11695"
     CDS             complement(2540..>3141)
                     /gene="prmC"
                     /locus_tag="AC159P_11695"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HIR3696153.1"
                     /translation="LIPRPETELLVETALGLLRPEESPAVCDVGTGSGCVAVAVLHER
                     AHARAVGLDISPAALRVAGRNAARHGVAARLRLLHSDCFDALRAKGRDDEDARFRMIV
                     SNPPYVAERDIEGLQREVRDHEPRLALTPGGDGLGVVRRLVAEAPDFLLPGGHLLFEF
                     GFDQHEQVAALINPRTWARRDILQDLQGIPRTALLRLAG"
BASE COUNT          487 a         1002 c         1116 g          536 t
ORIGIN      
        1 tggacgtgcg gcagcatttc cgcgagcgcg ccgcgccatg ccgcttcgtc gtggcggtcg
       61 aggaattctt cgaaagcggt caaagtcttg gtgctcatga ttcttcggtc gaaaaatgtc
      121 gtatgaatcg aaccggagag tttaacgatt ccgggcggca ggcacaaacc ggcagaagcc
      181 tttttatcga gactgcgcac gcgcgcgcgc ttcggcagcc ttccgccgcg gctacaggaa
      241 ccgggcgggc gcgtcgccga cgcgcccgcg gtgcaacact tgaccctctt gcgccggcgg
      301 gcgtaaattc taggctatga gctaccagga acctaccacc agaccgacca tcgaaaccct
      361 gttcaaaatc atgcgcgagg tgcgcgacga gatgcgcgcg gggttcgtgc gcgtcaacga
      421 ggggttcgga cgcatcgagt tgcggctcga aaaagtggag gagcagctcg accgggtgac
      481 gggcgtggcc cacgagacgc gtgcagacct gcgtgagttg aaaaaagacc tgcgcgatca
      541 cttcccggcg gtcaagtaag tttcggcggg aggttcaggg aaagaacggg cggcgagcag
      601 caagtctcgc cgcccgttct ttcgtctcac ggtcttctcg ttcagcccgg cggccacgcg
      661 agcgtgcgac cgccgaggac gtgcaggtgg atgtggaaga cggactgccc ggccccggcg
      721 cccgtgttga cgacggtgcg gtagccgctc tcggacaagc cctcctgacg ggcgacgcgc
      781 gcggcggcga gcatgagctg gccgagcttc gccgcgtccg tctcgctcgc gtcggcgagc
      841 gacgcgatgt gctcgcgcgg gatgacgagc acgtgcacgg gcgcctgcgg gttgatgtcg
      901 cggaaggcga ctgcgccgtc gtcctcgtag actttttcgg cgggcatccc gcccgcgact
      961 atcttgcaga agaggcagtt ttgttcgctc ataagaaaag gggctggagg cttgagactt
     1021 gaggcttgcg aaaggctgga ggcttgtgca agagaagaga ggctgttaac aagtctctag
     1081 cctctagcct ctagcctcta gcctccgcgt tgacgacggc ttcgaccggc ggcagttgcg
     1141 ccgtcacgga gtctctcagg cgctcgatgc gcgcgccgac ggagcggagt ttgtcttcga
     1201 tcttctcgta gccgcggtcg atgtggtaga cgcggtcgat gatggtctcg ccttcggcgg
     1261 cgaggccggc gaggacgagg caggccgagg cgcgcaggtc cgacgcctgc acgcgcgcgc
     1321 ccgcgagcgg cgtcgggcct tcgacgatgg ccgtgttgcc ctcgatctgg atggaggcgc
     1381 ccatgcgctg catctcggag gcgtgcatga agcggttctc gaagatcgtc tccttgatga
     1441 cggcgcgccc ctcggcctgc gtcatcaagg ccatgtactg cgcctgcatg tcggtgggga
     1501 agcgcgggtg cggctcggtc tccacgtcgc gcgcgaccag accctgcggc gagcggcgta
     1561 ccacgagcgt gctctggttg atctcttcga tctcgacgcc cgcctcgcgc agtttggcga
     1621 tgacggtggc cgagtgttcg gggcgacagt ctttgacttc gagctccccg cccgtaatcg
     1681 cggcggcgat gaggaaggtg ccggtctcga tgcggtcggg gatgatggtg tgctcggcgc
     1741 cgccgagctg ctcgacgccc tcgatggtga tggccggcgt gcccgcgccg cgcacgcgcg
     1801 cgcccatgcg gttcagcagc tccgccaagt cgctgatctc cggctcgcgc gcggcgttgt
     1861 tgatgacggt ctcgccttcg gcgagcgcgg cggccatcat gacgttctcc gtgcccgtca
     1921 ccgtcacctt gttgaagttg accgcggccc ctttgagccg cccgccctgc ggcgcgcgcg
     1981 cgacgacgtc gcccgcttcg agattgacct gcgcgccgag gagcttcagc gcttcgaggt
     2041 gcaggtcgat cggcctcgtc ccgatggcgc acccgcccgg caaggaaacc ttcgccttgc
     2101 cgaagcgcgc gagcagcggc ccgagcacga gcaccgacgc gcgcatcgtc ttcaccagct
     2161 cgtacggcgc ctcgaacagc tcgatgttcg aggccgtgat cttgtgcgtc cgcaggtctg
     2221 gcacgagcac cgtcgcgccc acgtcttcca aaaggcggcg ctgcgtcaga cagtcgcgca
     2281 cgtacggcac gttgtgcagc gtgacggtct cgcccgtcag cagcgcggcg gccatgcacg
     2341 gcagcgcgga gttcttcgcg ccgctgatct cgacgcgccc gctcaagggg cgcccaccgg
     2401 tgatccgaca tttatacaaa agcgtagagg tcagaggtca gaggtgagag gtcggaaaag
     2461 acagttcttc actgacctcc gacctccgac ctctgacctc tgaattttcg cggattctag
     2521 cacgcgcttc ctccggcggc tagccggcaa gccggaggag ggcggtgcgc gggatgcctt
     2581 gcaggtcttg gaggatgtcg cgccgcgccc acgtgcgggg gtttatcaac gcggcgacct
     2641 gctcgtgctg gtcgaagccg aactcgaaga ggaggtggcc gccgggcagg aggaagtcgg
     2701 gcgcctcggc gacgaggcgg cggacgacgc cgaggccgtc gccgccgggc gtgagcgcga
     2761 gccgcggctc gtggtcgcgc acctcgcgct gtagcccttc gatgtcccgc tcggcgacgt
     2821 aaggggggtt ggagacgatc atgcggaagc gcgcgtcttc gtcgtcgcgt cctttggcgc
     2881 ggagcgcgtc gaagcagtcg gagtggagga gccggagtct cgcggcgacg ccgtggcgcg
     2941 cggcgttgcg tccggccacg cgcagggctg cgggcgagat gtcgaggccg acggcgcgcg
     3001 cgtgcgcgcg ttcgtggagg acggcgaccg cgacgcagcc cgaacccgtg cccacgtcgc
     3061 agacggcggg cgactcttcc ggcctcaaaa gtccgagcgc cgtctcgacc agcagctccg
     3121 tctcgggccg cgggatgagc a
//
LOCUS       DBNGOH010000602         5582 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_456455, whole genome shotgun sequence.
ACCESSION   DBNGOH010000602 DBNGOH010000000
VERSION     DBNGOH010000602.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5582)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5582)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5582
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_456455"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..728)
                     /locus_tag="AC159P_11700"
     CDS             complement(<1..728)
                     /locus_tag="AC159P_11700"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020755762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR3696154.1"
                     /translation="MTVSVAMIVRDEERSLARCLDSFAREVDELVVVDTGSRDRTREI
                     ARRYTDRVFDFRWCDDFSAARQFAFDRATSDWVAWVDADDVVSNAAAIRPMTAAAPRE
                     VGGFQWRYVSDRDEWGNALCEFWRERCVRNDGTFRWEGRIHEVLVSSEPRQLPRSEEV
                     TIEHRRDPSRGPQKLRRNLRILERERREAGAQAGPRLFFYLANEYASAGRYEKALACY
                     RKYLRVATWDDERYVAEVRVANLNR"
     gene            complement(769..2319)
                     /locus_tag="AC159P_11705"
     CDS             complement(769..2319)
                     /locus_tag="AC159P_11705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696155.1"
                     /translation="MQDCCVSASADKALLNPNKRVNYTFGMVLGVDDFRQEQEHFEWK
                     HRLSNLLLHGTGTVCGLRVTAETVDGGADVEVRIAPGYAVSPHGRWVWVEKALCARLG
                     EWVAKNSITQSPPPGPGPQTVYVKLCYAECPTDLVPIAGQPCASEADTRAPSRVVETA
                     RAEFSWEPPDDHAEWHYREFGHLLSRVEVVEDVFSPGDAERFLEAVRALGRVDPLTTS
                     PPLSPPVTSPPAEGPQHEHHGTPHTHPQGSPPVSPDAAPFLLSEATACETIREALTIW
                     ATEICPHIHENLLRCAENDDCLLLACLHFELDFAGLLDVDSVRVEDCERPVLVSDRLK
                     QELFCISGRRGEDGATGPAGPTGASGPTGPTGATGATGATGATGLRGATGPTGPTGPT
                     GATGPTGATGATGATGTSVVSEFRTEVERIRGPFTVGLQTRVSERISHGLDERAMLSV
                     VVLDPQPIPAETRDEFPLGLNFAVTIYPDPEERGSMRIAATVLAPPNQLRVSFDSVTV
                     RWYALLPS"
     gene            complement(2338..4959)
                     /locus_tag="AC159P_11710"
     CDS             complement(2338..4959)
                     /locus_tag="AC159P_11710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696156.1"
                     /translation="MDVNGTRFHLIKGGADWRRCREEGPAGARAFRRLHFDEEAQVMR
                     LASQLVLFVSARGKAPLPLDARRGAAADPFQNWYWISNDRRRIYWRPLGSKKSRVFWS
                     QQPPPPDCKPSGDFKPVAEEAPSPVELSGLAVTSRHYLVVGNPAQRGLFVFDLHAGGP
                     PLLVRFPAGVPFEPFDMAPAPRGGVWILDRTNRAYWGLDRDFCVVSEGALHYEIAPEE
                     EEVFHVEEGTASVRPAKLFPRGFELPPDVENPVAIEGLPDGSVLLLDSPAYLDAVGSP
                     SAPSSVHHFRLGTSLGGPFELSDPEVGTVDELTGRVGTGLSVVAYDFAFAPAEEGATR
                     GTLHVVEREGNQAIAFTLDLEASPQRLAITPEFRPMHFFGSRALVQHRGRLFYDVVSG
                     DTANDRAVRWTELQGIDEQRFPVRATLLSHTLDGRERGCVWHRLFLDACIPAESRVEV
                     WSRAVDDERLLEQVEWSPEPGPYLRAAGPELPYFDAFPRYAPDELPENTGTWELLFQQ
                     ARGRFMQLRLVLSGNGRSSPHLHSLRAYYPRFSYPRNYLPNVYLEDAGSAHFLERMLA
                     NEEGFYSEIEGRIHDVSALFDARSAPPETLDWLAGWVGIVLDPLWADIQRGRQSETSA
                     LMPSRRKPFDRRRLFIRFTRLLFQRRGTPAGIHFALLLLLDPCLEVTLARLKRAAVRP
                     DDAKVAGLLEELKRYGLATPHPSMNDAEFEDLLYAYVLAPRRPSKVRIVERYRTRGGR
                     RLAEGDASVTSDTGQPVAGETPTAAEAPDAFAHQFSVLVPEALTPEEEAMVRRIAELE
                     KPAHTQFDVRRYWDFFRVGETRLGIDTVLGEESRFVEVILGRSYLAEGYLHPSHPADV
                     GERVVSDRDRLGDMPSL"
     gene            complement(5266..>5582)
                     /locus_tag="AC159P_11715"
     CDS             complement(5266..>5582)
                     /locus_tag="AC159P_11715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696157.1"
                     /translation="YLSALPPGGPDETGWPLNKHLLKKDLEAVVTRVAGVEFVNSMEL
                     GVEAELDIEDKHLKGLKLPRLDTLDVREGEAESLESILGAGAAEPQEPTVVPVPVTKS
                     KC"
BASE COUNT          839 a         1847 c         2056 g          840 t
ORIGIN      
        1 cgattgaggt tggcgacgcg cacctctgcg acgtagcgct cgtcgtccca agtggcgacg
       61 cgcaggtact tgcggtagca ggcgagcgcc ttctcgtagc ggcccgcgct cgcgtactcg
      121 ttggcgaggt agaagaagag gcgggggccg gcctgcgctc cggcctcgcg ccgttcgcgt
      181 tcgaggatgc ggaggttgcg gcgcagcttc tgcggcccgc gcgaggggtc gcggcggtgc
      241 tcgatggtga cctcttcgga gcgcgggagc tggcgcggct cggacgacac gagcacctcg
      301 tggatgcgcc cctcccagcg gaaggtgccg tcgttgcgga cgcagcgctc gcgccagaac
      361 tcgcacaggg cgttgcccca ctcgtcgcgg tcggagacgt agcgccactg gaagccgccc
      421 acttcgcgcg gggccgccgc ggtcatcggc ctgatggcgg cggcgttcga gaccacgtcg
      481 tcggcatcga cccacgcgac ccagtccgag gtcgcgcggt cgaaggcgaa ctggcgcgcg
      541 gctgagaagt cgtcgcacca tcggaagtcg aagacgcggt cggtgtagcg gcgcgcgatc
      601 tcgcgcgtgc ggtcgcgcga gcctgtgtcc acgacgacca gctcgtccac ctcgcgcgcg
      661 aagctgtcga ggcagcgcgc gagcgaacgc tcttcgtcgc ggacgatcat ggccaccgag
      721 actgtcatgg ctgctcctcc ttgagttaag ggcgcgcgct ccccgcggct acgacggcag
      781 cagggcgtac cagcgcacgg tcacggaatc gaaggaaacc cgcagttggt tcggcggcgc
      841 gaggaccgtg gcggcgatcc gcatcgagcc gcgttcttcg gggtccgggt agatggtgac
      901 ggcgaagttc agaccgaggg ggaactcgtc cctcgtctcc gccgggatgg gctgcgggtc
      961 gaggacgacg acggacagca tcgcgcgctc gtcgaggccg tgactgatcc tttcgctgac
     1021 gcgcgtctgc aaaccgacgg tgaacgggcc gcgaatcctc tcgacctcgg ttcggaactc
     1081 ggagaccact gatgtgccgg tggcgccggt cgcgccagtc gctccggtgg ggccagtcgc
     1141 ccccgtcggg ccggtggggc cggtggggcc ggtcgcgccg cgcaggccgg tggcgccggt
     1201 cgcgccggtc gcgccagtcg ctccggtcgg gccggtcggg cctgaagcgc cggtggggcc
     1261 ggcggggccg gtcgccccgt cctcccccct ccgcccgctg atgcagaaaa gctcctgctt
     1321 gaggcggtcg gagacgagca cgggtcgctc gcagtcttcg acccggacgc tgtccacgtc
     1381 gagcagaccc gcgaagtcga gttcgaagtg gaggcaggcg aggagcaggc agtcgtcgtt
     1441 ctccgcgcac cgcagcaggt tctcgtggat gtgcgggcaa atctcggtgg cccagatggt
     1501 gagcgcctcg cggatggttt cgcaggcggt cgcctcggag aggaggaagg gggccgcgtc
     1561 gggcgagacg ggcggcgagc cttgcgggtg cgtgtgcggg gtgccgtggt gttcgtgctg
     1621 cggcccttcg gcgggcggcg acgtgacggg cggcgagagc ggcggcgagg tggtcagagg
     1681 gtcaaccctg ccgagggcgc gcacggcttc gaggaagcgc tcggcgtcgc cgggcgagaa
     1741 cacgtcctcg acgacctcga cgcgcgagag caggtggccg aactcgcggt agtgccactc
     1801 ggcgtggtcg tcgggcggct cccaggagaa ctcggcgcgg gcggtctcga cgacgcgcga
     1861 gggggcgcgc gtgtcggcct cggaggcgca gggctggccg gcgatgggga cgaggtcggt
     1921 cgggcactcg gcgtagcaga gcttgacgta gacggtttgc gggccggggc cgggcggcgg
     1981 gctttgcgtg atgctgttct tggcgaccca ctcgccgagc cgcgcgcaca gcgccttctc
     2041 gacccagacc cagcgcccgt gcgggctgac ggcgtaaccg ggcgcgatgc gcacctccac
     2101 gtcggccccg ccgtccaccg tctcggcggt gacgcgcagg ccgcacaccg tccccgtccc
     2161 gtgcagcagc aggttgctca gtcggtgctt ccactcgaag tgctcctgct cctggcggaa
     2221 gtcgtccacg ccgagcacca tgccgaaggt gtagttgacc cgcttgttcg ggttcagaag
     2281 ggctttgtct gccgaagcag agacgcaaca gtcttgcatg attctctcca atcagtttta
     2341 gagcgacggc atatcgccca gacggtcacg atccgaaacc acgcgctcac ccacgtccgc
     2401 cgggtgtgag ggatgcagat agccctcggc caagtaagag cgaccgagga tgacctcgac
     2461 gaagcggctc tcctcgccga gcacggtgtc gatgccgagc cgcgtctcgc cgacgcggaa
     2521 gaagtcccag tagcggcgca cgtcgaactg ggtgtgcgcg ggcttctcaa gctccgcgat
     2581 gcgcctgacc atcgcctcct cttcgggcgt cagcgcctcg ggcacgagca ccgagaactg
     2641 gtgcgcgaag gcgtcgggcg cttccgccgc cgtcggcgtc tcgcccgcga ccggctggcc
     2701 cgtgtcgctc gtcaccgagg catcgccttc cgcgagcctg cgcccgccgc gcgtccggta
     2761 gcgctcgacg atgcgcacct tcgaggggcg gcgcggcgcg agcacgtacg cgtacaagag
     2821 gtcttcgaac tccgcgtcgt tcatcgaggg gtgcggagtc gcaaggccgt agcgcttcag
     2881 ctcttcgagc agcccggcga ccttcgcgtc gtcgggcctg acggcggcgc gcttcagccg
     2941 cgcgagcgtg acctccagac acgggtcgag cagtaggagc agcgcgaagt ggatgccggc
     3001 gggcgtgccc cgccgctgga acaggaggcg cgtgaagcgt atgaagaggc ggcggcggtc
     3061 gaagggcttg cgccgcgaag gcatgagcgc gctcgtctcc gactgccgcc cgcgctggat
     3121 gtcggcccac agagggtcga gcacgatgcc gacccagccc gcgagccagt cgagcgtctc
     3181 gggcggcgcg ctgcgcgcgt cgaagagcgc gctcacgtcg tggatgcgtc cctcgatctc
     3241 cgagtagaag ccctcctcgt tcgccagcat ccgttcgagg aagtgggccg agccggcgtc
     3301 ttcgaggtag acgttcggca ggtagttgcg cgggtacgag aagcgcgggt agtaggcgcg
     3361 aaggctgtgc aggtgcggcg acgagcgacc gttgcccgag agcacgagcc ggagctgcat
     3421 gaagcgaccg cgcgcctgct ggaacagtag ctcccacgtg cctgtgttct ccggcagctc
     3481 gtcgggcgcg taccgcggga aggcgtcgaa gtagggaagc tccggcccgg cggcgcgcag
     3541 gtacggcccc ggctcgggcg accactcgac ctgttcgagc agccgctcgt cgtccaccgc
     3601 gcgcgaccag acctcgacgc gcgactcggc ggggatgcac gcgtcgagga acagacggtg
     3661 ccagacgcag ccgcgctccc tgccgtcgag cgtgtgcgaa agaagcgtcg cgcgcacggg
     3721 gaagcgctgc tcgtcgatgc cctgtagctc cgtccagcgc acggcgcggt cgttggccgt
     3781 gtcgccgctc accacgtcgt agaagagccg cccgcggtgc tggacgagcg cgcggctgcc
     3841 gaagaagtgc atgggccgga actcgggcgt gatggcgaga cgctgcggcg acgcttcgag
     3901 gtcgagcgtg aaggcgattg cctggttgcc ctcgcgctcg acgacgtgca gcgtgccgcg
     3961 cgtcgcgccc tcttcggcgg gcgcgaaggc gaagtcgtag gcgacgaccg aaaggcccgt
     4021 gccgacgcgt cccgtcaact cgtccaccgt gcccacttcg gggtcgctca gctcgaacgg
     4081 cccgccgagg ctcgtgccca gacggaagtg atggacgctt gagggcgccg aggggctgcc
     4141 gaccgcgtcg aggtaagcgg ggctgtcgag gagaaggacg ctgccgtcgg gcagtccttc
     4201 gatggcgacc gggttctcga catccggggg aagctcgaag ccgcgcggga aaagcttggc
     4261 gggcctgacg gaggccgtgc cctcttcgac gtggaagacc tcctcctctt cgggcgcgat
     4321 ctcgtaatgg agcgcgcctt cggagacgac gcagaagtcg cggtcgagtc cccagtaggc
     4381 gcggttcgtg cggtcgagaa tccagacgcc gccgcgcggc gcgggcgcca tgtcgaacgg
     4441 ctcgaaggga acgcccgcgg ggaagcgcac gagaaggggc ggcccgcccg cgtgcaggtc
     4501 gaagacgaag aggccgcgct gcgccgggtt gccgacgacg aggtagtggc ggctcgtcac
     4561 ggccaggccg ctcagctcga cgggcgacgg cgcctcttcg gcgacgggct tgaagtcgcc
     4621 ggagggtttg cagtcgggcg gcggcggctg ctgcgaccag aagacgcgcg acttcttcga
     4681 acccagaggg cgccagtaaa tccgtcggcg gtcgttgctg atccagtacc agttctggaa
     4741 cggatctgcc gccgcgccgc gccgcgcgtc gagcgggagc ggcgccttgc cgcgcgcgct
     4801 gacgaagagc acgagctgcg aggcgagccg catcacctgc gcctcttcgt cgaagtgcag
     4861 gcggcggaac gcgcgggcgc ccgccggccc ttcctcgcgg cagcgccgcc agtccgcgcc
     4921 tcccttgatc aggtgaaagc gtgtgccgtt aacgtccacc taagaactgc tccctttcgt
     4981 cgcggttaag aatcacaacc cccggtcgtc ttcgactgcg gcttcgccgc ctgatgtgcg
     5041 gaagggctgt gcccttccgg cgactacgtt ttttaaccgg gggcgacgcc ccggaactga
     5101 ggcgcagcct cagttaaaaa caagacgacc tcaaggtgag gcgaagcctc accgcacatc
     5161 aggcggcaga gccgcaagaa cactcggagg ttggtcggac tttgtggtca aagaaagcat
     5221 gactaagaac ggccctcgtt tatccatgaa catttccgcg cgccgtcagc acttcgactt
     5281 cgtgacgggc acgggcacga ccgtcggctc ctgcggctcg gcggcgccgg cgccgaggat
     5341 ggattcgagc gactcggcct cgccctcgcg cacgtcgagc gtgtcgaggc gcggcagctt
     5401 caaccccttc aggtgcttgt cctcgatgtc gagttcggcc tcgacgccca gctccatcga
     5461 gttgacgaac tcgacgcccg cgacgcgcgt caccaccgct tcgaggtctt tcttcaagag
     5521 gtgcttgttg agcggccagc ccgtctcgtc cggcccgccc ggcggcagcg ccgaaaggta
     5581 ct
//
LOCUS       DBNGOH010000603         4749 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3389363, whole genome shotgun sequence.
ACCESSION   DBNGOH010000603 DBNGOH010000000
VERSION     DBNGOH010000603.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4749)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4749)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4749
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3389363"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..358
                     /locus_tag="AC159P_11720"
     CDS             <1..358
                     /locus_tag="AC159P_11720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696158.1"
                     /translation="NFPLMRLNVARTSDNFTLPDGRVVHGEFFTHLMYGSRGIDTFQF
                     HQTAPDRITLWVVPGEGEPEARARTLREAVAQIEALSAAPVTVEVRETDAIPLSSAGK
                     HRFTRSDVAAAASPVE"
     gene            367..2244
                     /gene="asnB"
                     /locus_tag="AC159P_11725"
     CDS             367..2244
                     /gene="asnB"
                     /locus_tag="AC159P_11725"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0070981 - L-asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HIR3696159.1"
                     /translation="MCGICGTYNPAGAAPEDESVVRRMMTLLEHRGPDGDGAWAAGQA
                     VLGHRRLAIIDLAGGHQPLFNETREVGAINNGEIYNYRELARELTAAGHRFRTASDTE
                     VIVHGYEEWGDDFVTRLRGMFAFAIWDEPRRRLLLGRDRFGVKPLYCAETKDRLLFAS
                     EIKALFADPSVPRRLNAARLAEYLAFRSVAGEETLFEGVREVAPGTVEVYEGGRRTVR
                     RYWSPEVRPTDEADAGETLGRGRELLSDAIGARLVSDVPLGTITSGGLDSSLVSAVAA
                     ELTPQPIDTFCVGFSDPAYDERPFARVVAEHIDSKHHAIEVSPADIERELDRLTWAHD
                     EPLTHPNSIPMHLIFRDAKERVGVTVLLSGEGADEVFGGYEWYAVARRRDALRGVPAL
                     SLLAAAAPPFGKLATLQKVMRRDYLFAANAVSSPAAVSALLPSGGGDYLRRRAQLWPT
                     GVEGADGMFVYDQRTYLAPLLQRQDRMSMAAGLEAREPFLDHHLAEWANALPSEVKLA
                     GGQRKALLKQLAARWLPAEIIHRRKVGFEMPLGAWLRRGGALAHRVQALRETGSPAGR
                     LVERAEVERLIAEHDRGAANHADLLWSLVALDSWAATFLGAGVRSQRLPGAATGKAVL
                     A"
     gene            2269..3453
                     /locus_tag="AC159P_11730"
     CDS             2269..3453
                     /locus_tag="AC159P_11730"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR3696160.1"
                     /translation="MKRNVLELTPSFHQGGSERQCVQLTRLLHEGGRHRVRLAALDGT
                     GPLRAEAESIGLGEVPSFPLTSFYDRNAAAQLRRFARLLREWQIDLVHAHDFYTNIFG
                     MAGAALARVPVRIASRRESAVRSARQRLVERAAYRLADAVVANCEEVRRQLVAEGVAA
                     DKTVTIHNGLDLRRVELPADFDRRETLAALGLPAEEGLRFVTILANLRIELKDHPTFL
                     RAASRVRAAVPEARFVLAGEGELASPLRALAAELGVGDSALFIGRCRRVAELLAVSDV
                     CVLSSKAEGFSNSILEYMAAGRPVVATDVGGAREAVVEGETGHLVASGDDEALAARIS
                     ALLGEPERARAMGARGRAVVEEKFSSAAQLERTLNLYERLLTEAHRGAARLLESVGRE
                     GA"
     gene            3477..4670
                     /locus_tag="AC159P_11735"
     CDS             3477..4670
                     /locus_tag="AC159P_11735"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR3696161.1"
                     /translation="MKTQQRESVEEAKGLGAAKDARRLRVLMVAPSLDMLGGQSRQCA
                     RLRGQLQHEPSLEVAFQPVNPRLPGPLRKLQSVKYVRTVVTTWLYWAQLVVRAWRSDI
                     LHVFSASYYSYMLSAMPAILVGKLYGKKVILNYRSGEAEDHLRNWRTAIPTIRLADAV
                     VVPSGYLVDVFARFGLKARAIHNIVELDRFRYRERRPLRPVFLTSRLLEPLYNVGCVL
                     RAFALIQREVPEASLTVAGEGWMRAELEQLARDLGLRHTKFIGGVPFEAMPDMYDGAD
                     IYLTATDLDNMPSSIVECLAAGLPVVTTDAGGIPYIVTHEETCLMIGRDDHEAMAAGA
                     LRLLSDGELAARIARNAREHCRKFSWPAVQREWLKLYHELVSPAAEREHRYTGGGPPS
                     AVRAE"
BASE COUNT          687 a         1718 c         1676 g          668 t
ORIGIN      
        1 caacttcccg ctgatgcgcc tgaacgtggc ccgcacgagc gacaacttca ccctgcccga
       61 cggacgggtc gtccacggcg aattcttcac gcacctgatg tacgggagcc ggggcatcga
      121 caccttccag ttccaccaga ccgcgcccga ccgcatcacc ctctgggtcg tgccgggcga
      181 gggggagccg gaggcgcgcg cgcgcacgct ccgcgaggcg gtggcgcaga tcgaggcgct
      241 ctcagccgcg cccgtcaccg tcgaggtgcg cgagacggac gcgatcccgc tctcctcagc
      301 cggcaaacac cgcttcacac gttccgacgt cgccgcggcg gcgtcgcccg tcgagtaaaa
      361 aattttatgt gtggcatctg tggcacctat aaccccgcgg gcgcggcgcc ggaagacgag
      421 tcggtcgtcc ggcggatgat gacgctgctc gaacaccgcg gccccgacgg cgacggcgcg
      481 tgggccgccg gccaggccgt gctcggccac cgccggctcg ccatcatcga cctcgcgggc
      541 gggcaccagc cgctcttcaa cgagacgcgc gaggtcggcg ccatcaacaa cggcgaaatc
      601 tacaactacc gcgagctggc ccgcgagctg acggcggcgg ggcatcgctt ccgcacggcg
      661 agcgacaccg aagtgatcgt ccacggctac gaggagtggg gcgacgactt cgtcacgcgc
      721 ctgcgcggga tgttcgcctt cgccatctgg gacgagccgc ggcgccgcct cctcctgggg
      781 cgcgaccgtt tcggcgtcaa gccgctctac tgcgccgaga cgaaagaccg cctgctcttc
      841 gcctcggaga tcaaggcgct gttcgccgac ccgtcggtgc cccggcgttt gaacgccgcg
      901 cggctcgccg aatacctcgc gttccgcagc gtcgcgggcg aggagacgct tttcgagggc
      961 gtccgggagg tggcccccgg aacggtcgag gtctacgagg gtggccggcg tacggtgcgg
     1021 cgctactggt cgccggaggt gcgtccgacc gacgaggccg acgccggcga aacgctcggg
     1081 cgcgggcgcg agctgctgtc cgacgccatc ggggcgcgcc tcgtctcgga cgtgccgctc
     1141 ggcacgatca cgagcggcgg gcttgactcc agcctcgtca gcgccgtggc cgccgagctg
     1201 accccgcaac cgatagacac cttctgtgtc gggttctccg acccggccta cgacgagcgc
     1261 ccgttcgcgc gcgtcgtggc cgagcacatc gactcgaagc accacgccat cgaggtctcg
     1321 cccgccgaca tcgagcgcga actcgaccgc ctgacgtggg cgcacgacga gccgctgacg
     1381 cacccgaact cgatcccgat gcacctcatc ttccgcgacg ccaaggagcg cgtgggcgtg
     1441 accgtgctgc tctcgggcga gggggccgac gaggtcttcg gcggctacga gtggtacgcg
     1501 gtcgcccgcc gccgcgacgc gttgcgcggg gtgccggccc tctccctgct cgccgctgcc
     1561 gcgccgccct tcggcaagct cgcgaccctc cagaaggtga tgcgccgcga ctacctcttc
     1621 gcggccaacg ccgtctcctc gccggctgcc gtctcggcgc tgctcccgtc cggcggcggc
     1681 gactacctgc gacggcgcgc gcagctgtgg ccgacgggcg tcgagggcgc cgacggcatg
     1741 ttcgtctacg atcagcgcac ctacctcgcg ccgctgctcc aacgccagga ccgaatgagc
     1801 atggcagccg ggctggaggc gcgcgagccg ttcctcgatc atcacctcgc cgagtgggcc
     1861 aacgccctgc cgtccgaggt gaagctggcc ggcgggcagc gcaaggcgct gctcaaacaa
     1921 ctcgccgcgc gctggctccc cgcggagatc atccaccgcc gcaaggtcgg cttcgagatg
     1981 ccgctcggcg cgtggctgcg gcgcgggggc gcgttggccc accgcgtgca ggctttgcgc
     2041 gaaacaggct cgcccgcggg ccggctcgtc gagcgggcag aggtcgagcg cctcatcgcc
     2101 gagcacgacc gcggggccgc taaccacgcc gacctcctct ggtcgctcgt cgcgctcgac
     2161 tcctgggcgg cgaccttcct cggcgccggc gtccggagcc agaggctgcc cggagccgcg
     2221 accgggaaag cggtgctcgc ctgagcggca tgggggagga gtgagggttt gaaacgcaac
     2281 gtcttagaac tgacccccag cttccatcag ggcggctccg agcggcagtg cgtgcagctc
     2341 acgcgcctgc tgcacgaggg cgggcgccac cgcgtccgcc tggccgccct cgacggaacc
     2401 ggcccgctgc gcgccgaggc cgaaagcatc ggcctcgggg aagtcccgtc gttcccgctg
     2461 acgagctttt acgaccgcaa cgccgccgcc caactgcgcc gcttcgcgcg cctgctgcgc
     2521 gagtggcaga tcgacctcgt ccacgcacac gacttctaca ccaacatctt cggcatggcg
     2581 ggcgcggccc tggcgcgcgt gcccgtccgc atcgcctcgc ggcgtgagtc agccgtgagg
     2641 agcgcgcgcc agcgcctcgt cgagcgcgcg gcctaccggc tggccgacgc cgtcgtcgcc
     2701 aactgcgagg aggtgcggcg gcagctcgtc gccgagggcg tcgccgccga caagacggtc
     2761 acgattcaca atgggttgga cctgcggcgg gtggagctgc cggcggactt cgaccgccgc
     2821 gagacgctgg cggcgctcgg cctgcccgcg gaagagggct tgcgcttcgt gaccatcttg
     2881 gcgaacctgc gcatcgagct gaaggatcac ccgacgtttt tgcgcgcggc gagccgcgtg
     2941 cgcgcggcgg tgcccgaggc gcgcttcgtc cttgcgggcg agggcgagct ggcgagtccc
     3001 ttgcgcgcgc tggccgccga actcggcgtg ggcgactccg ccctcttcat cggtcgctgc
     3061 cggcgcgtcg ccgaactcct ggcggtctcc gacgtgtgcg tgctcagctc gaaggccgag
     3121 ggcttctcga actcgatcct cgaatacatg gcggcggggc gtcccgtcgt ggcgacggac
     3181 gtgggcggcg cgcgcgaggc ggtcgtcgag ggcgagacgg ggcacctcgt ggcctccggc
     3241 gacgacgagg cgctggcggc gcgcatctcc gcgctgctcg gcgaaccgga gcgggcgcgc
     3301 gcgatgggcg cgcgggggcg ggcagtggtc gaagagaagt tttccagcgc ggcgcaactg
     3361 gagcgcacgc tgaacctgta cgagcgcctg ctgacggaag ctcaccgcgg cgcggcccgc
     3421 ctgctggaga gcgtggggcg cgagggcgct tgagcgccgg ggccgggaag aagacgatga
     3481 agacgcagca gagagagtcg gtcgaagagg caaaggggct gggggcggcg aaagacgcgc
     3541 ggcgcctgcg cgtcttgatg gtcgcgccgt cgctcgacat gctgggcggg cagtcgcgcc
     3601 agtgcgcgcg gctgcgcggg cagttgcagc acgagccgtc gctcgaagtc gccttccagc
     3661 ccgtcaaccc gcggctgccc ggcccgctcc gcaaactcca gtccgtcaag tacgtccgca
     3721 cggtcgtgac gacctggctc tactgggcgc agctcgtcgt gcgcgcctgg cgctcggata
     3781 tcctccacgt cttctcggcc tcctactact cctacatgct gtcggcaatg ccggccatcc
     3841 tcgtcggcaa gctctacggc aagaaggtga tcctcaacta tcgcagcggc gaggccgaag
     3901 accacctgcg gaactggcgc acggccatcc cgacgatccg gctcgccgac gccgtcgtcg
     3961 tcccctcggg ctacctcgtc gatgtcttcg cgcgcttcgg gctgaaggcg cgcgccatcc
     4021 acaacatcgt cgaactggat cggttccgct accgcgagcg ccgcccgctg cggcccgtct
     4081 tcctgacgag ccggctgctg gagccgctct acaacgtcgg ctgcgtgctg cgcgcgttcg
     4141 cgctcatcca gcgcgaggtg cccgaggcaa gcctgaccgt ggcgggcgag ggctggatgc
     4201 gcgccgagct ggaacagctc gcgcgcgacc tcgggctgcg ccacacgaag ttcatcggcg
     4261 gcgtgccgtt cgaggcgatg cccgacatgt acgacggagc ggacatctac ctgacggcga
     4321 ccgacctcga caacatgccc tcgtccatcg tcgagtgcct tgccgccggg ctgccggtgg
     4381 tcacgaccga cgcgggcggc atcccctaca tcgtcacgca cgaggagacg tgcctgatga
     4441 tcgggcgcga cgaccacgag gcgatggccg ccggcgcgct ccgcctgctc tcggacgggg
     4501 agctggccgc gcgcatcgcg cgcaacgctc gcgagcactg ccgcaaattc agttggcccg
     4561 cggtgcagcg cgagtggctg aagctctacc acgaactcgt cagccccgcc gccgagcgcg
     4621 agcaccgcta cacagggggc ggcccgccga gcgcggtgcg tgccgaatga ggcggagcgc
     4681 gccgcccgac gggccacgcc gccgtatgca gatgaacccc atcctggaga agctgaagaa
     4741 actgaaggg
//
LOCUS       DBNGOH010000604         3852 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_822078, whole genome shotgun sequence.
ACCESSION   DBNGOH010000604 DBNGOH010000000
VERSION     DBNGOH010000604.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3852)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3852)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3852
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_822078"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2781
                     /locus_tag="AC159P_11740"
     CDS             <1..2781
                     /locus_tag="AC159P_11740"
                     /inference="COORDINATES: protein motif:HMM:NF051698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HIR3696162.1"
                     /translation="LASPRTTARIIGYDRTNRTGAGAITTRAFQFGNAPVASRALFED
                     NPLVQQLNVLPSEFEQHQFTARLDGRLTSKNTLTGTFFFSNFPALDSFPDPTSLVSPY
                     VLRRADRNRTLAVSDQHIFSSSLINEARFGYFSLNNTRALDEPYLAQELTSEAVGISN
                     PALLFDNSPATRRLGHFIGRPGTNLSQFSFGGPNDSFNKRKQQTYSFSDNVTWINGAH
                     TLRFGGDFKSHRYDSSLPEEQATEFEKFDSITQLVTGNATEADTQFGITAKSFRFKDY
                     SGYVADDWKLSRKVTLNLGLRYELFMWPTEKDGRIGNFDFDAFDQCFSQTGGPLSLCD
                     NPAPGFIVPSNVRETGLGNVDGAIAATARVENDHTLRGQDTNNFAPRVGFAYSPFDSN
                     RVVVRGGFGLFYDRPSAAFINTVFSNYPFLREVEITVPSGNVPIATAFGAQPTALPLS
                     NWLPFRIVRAAGTGGSYVIRDNTGVTADARGNPTVGANLGNIAETFEFRAIDRDLKTP
                     YVQQWNLGVQYEVTKNLLFEARYVGTRGRNLLQALAFNQGYDLNDPSTPDHIYERFNQ
                     AYLAAGSPNGPLNAGSTARERGTGRAFGFANPYRVGQTATCAGGVLGGVAGAPLDLNL
                     ANPITCAGTTIGGGQIINFEARVPVLGFNVPEALLLQSNGNSIYHGAQFSLTRRFSDG
                     LQFNAAYTFSKSLDTSSTDPGSTAGSGKPDVPNTGFVVQGDQRHVESSRAVSDFDRTH
                     RFSLNFVYQLPGYASSNKFLNGWQLSGFFQAQTGTPYSIFAPEPELQTAPQYADLVRG
                     SGGLYRLGFGRPALCGSLDQLRQEGTDVTEDAFDASGTSALCTAFGQNGSLGRNVLRA
                     RAQHRFDLGLVKSTKLTETTSLELGWDVFNVFNRANFAAPDSELGSPDFGRITNTTGG
                     PRVMQFRAKLKF"
     gene            2926..3303
                     /locus_tag="AC159P_11745"
     CDS             2926..3303
                     /locus_tag="AC159P_11745"
                     /inference="COORDINATES: protein motif:HMM:NF012698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HIR3696163.1"
                     /translation="MSTELATFGRGGMDAGAQAEARVYDDGHLRVEHDNYYVSLGGQV
                     LKLARKEFLILSRLAQGAERIVASEELWRHAWAGRAPFNAESMHVHIYRLRRKLAPHG
                     LQIETMVNVGYRLVSPKVPRTED"
     gene            3497..>3852
                     /locus_tag="AC159P_11750"
     CDS             3497..>3852
                     /locus_tag="AC159P_11750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011140012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HIR3696164.1"
                     /translation="MSFRKHLKRAALSLISALALLSAGCGAPPAGDANKAGGGENFIK
                     IDGSSTVFPVTEAVAEEFQKEKGGKVKVTVGISGTGGGFKKFARGETDISDASRPILA
                     EEMAQAKQNGIEFVELP"
BASE COUNT          684 a         1460 c         1094 g          614 t
ORIGIN      
        1 ctcgcctcgc cgcgcacgac ggcgcgcatc atcggctatg accgcaccaa ccgcacgggc
       61 gcaggcgcca tcacgacgcg cgccttccag ttcggcaacg cgcccgtcgc gagccgcgcg
      121 ctcttcgaag acaacccgct cgtccagcag ctcaacgtgc tcccctccga gttcgagcag
      181 caccagttca ccgcgcgcct cgacgggcgg ctgacctcga agaacacttt gaccggcacc
      241 ttcttcttct cgaacttccc cgcgctcgac tcgttcccgg acccaacgag cctcgtctcg
      301 ccctacgtcc tcaggcgcgc cgaccgcaac cgcacgctcg ccgtctccga ccagcacatc
      361 ttcagctcgt cgctgattaa cgaggcgcgc ttcggctact tctctctgaa caacacgcgc
      421 gctctggacg agccgtacct cgcgcaggag ttgacgagcg aagccgtcgg catctcgaac
      481 ccggcgctcc tcttcgacaa cagccccgcg acgcgccgcc tcggccactt catcgggcgg
      541 cccggcacca acctctcgca gttctccttc ggcggcccga acgactcctt caacaagcgc
      601 aagcagcaga cctacagctt ctccgacaac gtgacgtgga tcaacggcgc gcacaccctc
      661 cgcttcggcg gcgacttcaa gagccaccgc tacgattcga gcctgcccga ggagcaggcc
      721 accgagttcg agaagttcga cagcatcacg cagctcgtca ccggcaacgc caccgaggcc
      781 gacacgcagt tcggcatcac cgcgaaatct ttccgcttca aggactacag cggctacgtc
      841 gccgacgact ggaagctctc gcgcaaagta acgctgaacc tcggcctgcg ctacgagctg
      901 ttcatgtggc ccaccgaaaa ggacgggcgc atcggcaact tcgacttcga cgccttcgac
      961 cagtgcttct cgcagacggg cggcccgctc tcgctgtgcg acaacccggc gcccggcttc
     1021 atcgtcccgt cgaacgtgcg cgagaccggc ctcggcaacg tggacggcgc catcgccgcg
     1081 acggcgcgcg tcgagaacga tcacacgctc cgcggtcagg acacgaacaa cttcgccccg
     1141 cgtgtcggct tcgcctactc gcccttcgac tcgaaccgcg tggtcgtgcg cggcggcttc
     1201 ggcctcttct acgaccgccc ctcggcggcc ttcatcaaca cggtcttctc caactacccc
     1261 ttcctgcgcg aggtcgagat caccgtgccc tccggcaacg tgccgattgc gacggccttc
     1321 ggcgcacagc ccacggcgct ccccttgagc aactggctcc ccttccgcat cgtgcgcgcc
     1381 gcgggcaccg gcggctccta cgtcatccgc gacaacaccg gcgtcacggc ggacgcgcgc
     1441 ggcaacccga ccgtcggcgc caacctcggc aacatcgccg agaccttcga gttccgcgcc
     1501 atcgaccgcg acctcaagac gccttacgtc cagcagtgga acctcggcgt gcagtacgaa
     1561 gtcacgaaga acctcctctt cgaggcgcgc tacgtcggca cgcgcgggcg caatctgctt
     1621 caggcgctcg ccttcaacca gggctacgac ctcaacgacc cctcgacgcc cgaccacatc
     1681 tacgagcgct tcaaccaggc gtacctcgcc gccggctcgc ccaacggccc cttgaacgcc
     1741 ggttcgaccg cgcgcgagcg cggcacgggt cgcgccttcg gcttcgccaa cccgtaccgc
     1801 gtcggccaga cggccacctg cgcgggcggc gtgctcggcg gcgtcgcggg cgctccccta
     1861 gacctcaacc tcgccaaccc gatcacctgc gcgggcacca ccatcggcgg cggccagatc
     1921 atcaacttcg aggcgcgcgt ccccgtcctc ggcttcaacg tgccggaagc gctgctgctt
     1981 cagtcgaacg gcaactcgat ctatcacggc gcccagttca gcctgacccg gcgcttctcc
     2041 gacggcttgc agttcaacgc ggcctacacc ttctcgaagt cgctcgacac cagctcgacc
     2101 gaccccggca gcaccgccgg ctcgggcaag cccgacgtgc ccaacacggg cttcgtcgtg
     2161 cagggcgacc agcgccacgt cgaaagcagc cgcgccgtct ccgacttcga ccgcacgcac
     2221 cgcttcagcc tcaacttcgt ctaccaactg ccgggctacg cttcgtcaaa taagttcctc
     2281 aacggctggc agctctccgg cttcttccag gcgcagaccg gcacgcccta cagcatcttc
     2341 gcgcccgagc ctgagctaca gaccgccccg cagtacgcag acctcgtgcg cggctccggc
     2401 ggcctctacc gcctcggctt cggcaggccc gcgctgtgcg gctcgctcga ccaactgcgc
     2461 caggagggca cggatgtcac cgaagacgcc ttcgacgcct ccggcacctc ggccctctgc
     2521 acggccttcg gccagaacgg cagcctcggg cgcaacgtcc tgcgtgcgcg cgcgcagcac
     2581 cgcttcgacc tcggcctcgt caagagcacg aagctcaccg agacgacttc gctcgaactc
     2641 ggctgggacg tcttcaacgt cttcaaccgc gccaacttcg ccgctcccga cagcgaactc
     2701 ggcagccccg acttcggtcg catcaccaac accacgggcg gcccccgcgt catgcagttc
     2761 cgggccaagc tgaagttcta agggccgtga gccgtgaacc gtaaaccgtg acgagtaaaa
     2821 acccatctcc tcttgtcacg gtttacggtt cacccggcac ggcaaccact caacgcgctt
     2881 cacggctttt cacttatcac ggttcacggt tcacggtatt catctatgag cacagagctg
     2941 gcaacgttcg ggcggggcgg catggacgcg ggcgcgcagg cggaggcgcg cgtgtacgac
     3001 gacggccacc tgcgcgtcga gcacgacaac tattacgtga gcctcggcgg gcaggtcttg
     3061 aagctcgcgc gcaaggagtt cctcatcctc tcgcgcctcg cgcagggcgc cgagcgcatc
     3121 gtcgcgtccg aagagctgtg gcgccacgcg tgggcgggtc gcgcgccctt caacgccgag
     3181 agcatgcacg tccacatcta ccgcctgcgc cgcaagctcg cgccgcacgg cctacagatc
     3241 gagacgatgg tcaacgtcgg ctaccgcctc gtctccccaa aagtaccgag gactgaggac
     3301 tgagtcgaag tgcgtgacgg atgtagagag tggtgctgta cggactcagc gcggctcatg
     3361 tctttcacag tcctcagtcc tcagtccttt cgttcggtca cataaatttc agctccagtt
     3421 cagacggatg taacacgacc ggcctaaaat tcccgattca agcttccgtc aaccccccat
     3481 tcaaggagaa gtcagcatgt cctttcggaa acatctcaaa cgtgccgcgc tgtcgctcat
     3541 ctcggcgctc gcgcttttgt cggcgggctg cggcgcgccg cccgcgggcg acgccaacaa
     3601 agcgggtggg ggcgagaact tcatcaagat cgacgggtcg agcacggtct ttcccgtcac
     3661 cgaggccgtc gccgaggagt tccagaagga gaaggggggc aaggtcaagg tgacggtcgg
     3721 catctccggc accggcggcg ggttcaagaa gttcgcccgc ggcgagaccg acatctccga
     3781 cgcctcgcgc ccgatcctcg ccgaggagat ggcgcaggcc aagcagaacg gcatcgagtt
     3841 cgtcgagctg cc
//
LOCUS       DBNGOH010000605         2475 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1373860, whole genome shotgun sequence.
ACCESSION   DBNGOH010000605 DBNGOH010000000
VERSION     DBNGOH010000605.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2475)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2475)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2475
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1373860"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            62..442
                     /locus_tag="AC159P_11755"
     CDS             62..442
                     /locus_tag="AC159P_11755"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3696165.1"
                     /translation="MKVFVAEDNELLRARLVNLLSEFPGVEIIGEARDAHEALRAIRE
                     TRPDVVTLDIHMCGGSGISVLKEIKREEASPVVLMLTNHVSPPYRKCCMEAGADFFLD
                     KANGLDRVRDIFQTLLGRSDATAT"
     gene            complement(618..1637)
                     /locus_tag="AC159P_11760"
     CDS             complement(618..1637)
                     /locus_tag="AC159P_11760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HIR3696166.1"
                     /translation="MRVVTFGEIMMRLATPGRLRFSQAKELELTYGGGEANVAVSLAN
                     FGLDASFVTRLPKNPFGDGAVNTLRGLGVDTSHVARGGERIGIYFLETGASQRASVVV
                     YDRAASAISQVGPGEFDWPAIFEGAGWLHFTGITPALGPRVAEATAEACRAAKAAGLT
                     VSCDLNYRKKLWTPAQAQETMTGLMEFVDVAIGNEEDAEKVFGIKSETANVEGGHVSA
                     EGYEQVARTMAERFGLKAVAITLRESRSADSNGWSAMLLHEGQVYQSRKYEVWIVDRV
                     GGGDSFAGGLIYGLATNKSPQEALEFAVAASCLKHSIAGDFNLVSVAEVEALVKGDAS
                     GRVQR"
     gene            complement(1696..2343)
                     /locus_tag="AC159P_11765"
     CDS             complement(1696..2343)
                     /locus_tag="AC159P_11765"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007961273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-keto-4-hydroxyglutarate
                     aldolase/2-keto-3-deoxy-6-phosphogluconate aldolase"
                     /protein_id="HIR3696167.1"
                     /translation="MIPKLDILRRITEAGLVAVVRAESSDQALRVSDAVLAGGCPAVE
                     VTFTVPRAHRVIEALAERYKPSELILGAGTVLDPETARVAILSGATYVVSPALNTETV
                     RLCNRYQVPVMPGVMTIRDVIEAMEAGADIVKLFPGEAFGPAMIKAIKGPLPQAPLMP
                     TGGVDVKNVGEWIKAGAVAVGAGSSLTGGAKSGDYDAVTRTAREFIEKIKEARGA"
BASE COUNT          406 a          888 c          765 g          416 t
ORIGIN      
        1 ccaaccttca gtgcagcgcg agccgccgaa tcagctctcc ggggcactag cgagcattac
       61 gatgaaagta ttcgtcgcgg aagacaacga gctgctgcgc gctcgactgg tgaacctcct
      121 ctccgaattc cccggcgtcg agatcatcgg cgaggcgcgc gacgcgcacg aagcgctgcg
      181 cgccatccgc gagacgcgcc ccgacgtggt cacgctcgac atacacatgt gcggcggcag
      241 cggcatcagc gtcctcaagg agatcaagcg cgaggaggcg tcgcccgtcg tgctcatgct
      301 caccaaccac gtctcgccgc cctaccgcaa gtgctgcatg gaggccggag ccgacttctt
      361 cctcgacaag gcgaacggcc tcgaccgcgt cagggacatc tttcaaaccc tcctcgggcg
      421 ctccgacgcg accgcgacct agacaggttg tcgtcgagtt taccgggacg acgaattaag
      481 gcagagaacg cggaggacac gcggagggca cagagaaagc aattggtttt ctctgcgccc
      541 tccgcgtgtc ctccgcgttc actgcgccga gatgaacccg cgccacgctc aaccgccgac
      601 cgctccggct cggcccctta ccgctggacg cggcccgacg cgtcgccctt cacgagcgcc
      661 tcgacctccg ccaccgagac caggttgaag tcgcccgcga tcgagtgctt caggcacgac
      721 gccgccaccg cgaactccaa cgcttcctgc ggcgatttgt tcgtcgccaa cccgtagatc
      781 agcccgcccg cgaacgagtc gccaccgccc acgcggtcaa cgatccacac ctcgtacttg
      841 cgcgattgat aaacttgacc ttcgtgcaac agcatcgccg accacccgtt cgagtccgcc
      901 gaacggctct cgcgcaaagt gatcgccacc gccttcaacc cgaaccgctc ggccatcgtc
      961 cgcgccacct gctcgtagcc ctccgccgag acgtgcccgc cttcgacgtt ggccgtctcc
     1021 gacttgatgc cgaacacctt ctcggcgtcc tcctcgttgc cgatcgccac gtctacaaac
     1081 tccatcagcc ccgtcatcgt ctcctgcgcc tgcgcgggcg tccacagctt cttgcggtag
     1141 ttcaggtcgc acgagaccgt gaggcccgcc gcctttgccg cacggcacgc ctcggcggtg
     1201 gcctcggcca cccgcggccc cagcgcgggc gtgatgcccg tgaagtggag ccacccggca
     1261 ccctcgaaga tcgcaggcca gtcgaactcg cccggcccga cctgcgagat cgccgaagcc
     1321 gcgcggtcgt agacgaccac ggaggcacgc tggctggcgc ccgtctccaa aaagtagatg
     1381 ccgatgcgct cgccgccgcg cgcgacgtgc gaggtatcga cgccgaggcc gcgcaaggtg
     1441 ttcaccgcgc cgtcgccgaa cgggttcttg ggaagacgcg tgacgaagct cgcgtcaagg
     1501 ccgaagttgg cgagcgagac ggcgacgttg gcctcgcccc cgccgtaggt caattcaagc
     1561 tctttagctt gggagaagcg caaacggccc ggcgtggcca gccgcatcat gatctcgccg
     1621 aacgtcacca ctcgcatctc tcttcggtct ccttcttctt tactcactcg ccgacttccg
     1681 cggcccctca cgcgctcacg cgcctctcgc ctctttgatc ttctcgatga actcgcgggc
     1741 ggtgcgcgtg acggcgtcgt agtcgcccga cttggcgccg cccgtcaggc tgctgcccgc
     1801 gccgacggcg accgcgcccg ccttgatcca ctcgccgacg ttcttcacgt ccacgccgcc
     1861 ggtcggcatc agcggggcct gcgggagcgg ccccttgatg gccttgatca tcgcggggcc
     1921 gaacgcctcg ccggggaaga gcttgacgat gtcggcgccc gcctccatcg cttcgattac
     1981 gtcgcggatg gtcatcacgc cgggcatgac gggcacctgg tagcggttgc agagccgcac
     2041 ggtctccgtg ttgagcgcgg gcgagacgac gtaggtggcg ccggagagga tggcgacgcg
     2101 ggcggtctcg gggtcgagga cggtgccggc gccgaggatc agctcggaag gcttgtagcg
     2161 ttcggccagg gcttcgatga cgcggtgcgc acgcgggacg gtgaaggtga cttcgacggc
     2221 ggggcagccg cccgcgagga cggcgtccga gacgcgcagc gcctggtctg aggactcggc
     2281 gcgcactacg gcgacgaggc cggcctccgt aatccgccgc aggatgtcca gtttcgggat
     2341 catgtcggtc gtctcttctt gaaagggatt gcggattgcg gattggcgtg ctgctctgac
     2401 tcgaagcttg atttttgacg ctcgaccttc gtcgctcgtt caatccgcaa tccgcaatcc
     2461 gcaatccgca atccg
//
LOCUS       DBNGOH010000606         4992 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2656256, whole genome shotgun sequence.
ACCESSION   DBNGOH010000606 DBNGOH010000000
VERSION     DBNGOH010000606.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4992)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4992)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4992
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2656256"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..462)
                     /locus_tag="AC159P_11770"
     CDS             complement(<1..462)
                     /locus_tag="AC159P_11770"
                     /inference="COORDINATES: protein motif:HMM:NF014119.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HIR3696168.1"
                     /translation="MPKTRTTEDRANGADATGGDATSNGGDATCDEPRGHSRSRHRLS
                     HQRSRLAFLLNDALDTLTGSPLTKAIRRRLARLTRSETEAAALAPHLDLGRRGELLAA
                     ERLRREGYLPVASNFELNVGRNRRGALVRAEIDLVAYEGRTLCFVEVKTRAS"
     gene            682..2157
                     /gene="der"
                     /locus_tag="AC159P_11775"
     CDS             682..2157
                     /gene="der"
                     /locus_tag="AC159P_11775"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008341048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HIR3696169.1"
                     /translation="MDASEEPSATAVETDEVGEQQRSEAAVPATAPQALPLVAIVGRP
                     NVGKSTLFNRLVGERRSIVGDEPGITRDRIYGETDWAGRRFSIVDTGGIVPDETEVIP
                     ANILRQAEFAITEAVALVWVVDVRAGLTPLDEELARLLRTTDKTIIVAANKADALKLE
                     AHAGEFHRFGFEDVVPISAEQGDGVAELLDVLVEHLPSFEEMGLEFAQTEAQVRSGEV
                     RLAIVGRPNVGKSSLLNRLLGEERVIVSPVAGTTRDAVDTVLQHEGKNFRLVDTAGIR
                     RKGKTEAMAEKLSVVMARRSLERADVAVVVVDAVDGVAALDANIAGYAVDAGCSVIIA
                     VNKWDALANKETGTPAAFERDLRLKMKFLEWAPVVTISALTGQRVDRLLQLALHANEA
                     RNRRIPTSRLNEFFERAVASPRAPTAPAPVKGGRSRLHVQYVTQVGVRPPRFVVFTAG
                     GRAGIHFSYERYLQNRLREEFDFFATPLRIVERHRRERRKP"
     gene            2216..4951
                     /locus_tag="AC159P_11780"
     CDS             2216..4951
                     /locus_tag="AC159P_11780"
                     /inference="COORDINATES: protein motif:HMM:NF013736.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HIR3696170.1"
                     /translation="MEALDASNLRKLFWHTSEPAYVVDAATKCVLAANPPFAELTKRT
                     PDALVGAKEDALLAFEDGDQEGAAGEWARLLAGADGGGHEGDGVRVRLESFPCVWEGK
                     AACLYFVRRADAGGDGLRVEAEGDRARALLDYLHEATDQLEVINRVVAAVNAGSTIED
                     VFRPASEQIRTLVPFDRASIALLDAGSSLRVFALSGEAGSLAVGATAPLRGSVTETAL
                     AGRQMVSIPELAEETRFNVYEDLRREGFRSAVCVPLFSMSRAVGSLNLTSRTPDAYKR
                     RHLAALERLAPPFAIAIEKVLLLEASRHRTEELRGLFEISRAFSTLTDTSEISSRLAR
                     AIAKLVGGTMCLIATYDRRRNAVRAESPGYRTPPELVREFQFTLDRGNHHAPLYQVGE
                     AFLSNDPARDARLNRRFVERWGVHNVLSVPMRIKKELIGFIYVANREGSFSERDLRLL
                     EIFAAQAAETIVNARLFLTIQAQAEREAVVNRLLLSLQRSGEPKEKVSAVIERIGEVL
                     ELDRCVAVLFADGDHDDYYGEWCADGVEPVTDWMDMRERSPIRYALRTARRPLYATDV
                     REHPLAPGLEDLIERSNLKSLLVVPIIHQGRVIGSISGHQTRTFRNWNEDDIDLLVAV
                     ATHVGATLENARLIAELREANRLKDEFLATLSHELRTPLTAITGWVEILAEAHRAHKD
                     EDVADGINAISASATSLAQLISDLLDLSRIQRGVLRLERQFFDVNQVVAGAARNVRQA
                     ALARRLDLRLELAENVPTTIADTQRVQQVLWNLLMNAIKFTPQGGAVTVRTRLVEDWP
                     QGGEGEGAERDARGLVRWVEVEVEDTGEGIPEDFMPYIWDRFRQADGSATRRHGGLGI
                     GLSLVKELIEAHGGQVNAHSQQRGARFNVRLPVVSVEGWLAHEPRFERDEYDGQR"
BASE COUNT          785 a         1666 c         1804 g          737 t
ORIGIN      
        1 ggaggcgcgc gtcttgacct cgacgaagca gagggtgcgg ccctcgtagg cgacgaggtc
       61 gatctcggcc cggacgagtg cgccgcggcg gttgcgaccg acgttgagtt cgaagttgga
      121 ggctacgggc aggtagcctt cgcgccggag gcgttcggcg gcgagcagtt cgccgcggcg
      181 cccgaggtcg aggtgtggcg cgagcgcggc ggcttcggtc tcggagcgtg tgagacgggc
      241 gaggcgtcgc cggatggctt tcgtcagagg cgagccggtg agcgtgtcga gggcgtcgtt
      301 caagaggaag gcgaggcgcg agcgttgatg ggagagccgg tggcgggaac gagagtggcc
      361 gcgcggctcg tcgcacgtcg catcgccgcc gttgctcgtt gcgtcgccgc cggttgcgtc
      421 ggcgccgttc gcgcggtctt cggtcgtgcg agtcttgggc atcgccgtgc gggaagtgtt
      481 gttgtgtggc caggtgtcgg agcggcggga cgatggcaag cgtaaacccg cgtggccgtc
      541 ctggcaagcg gaatgtgcga gggcgaacct tgccccggtc ggggccgcac gctacacttc
      601 gttcgtcccc ctcacgccct tggttcgttc cccccggaga aaaggaaggc gcgcgtgtgt
      661 gcgcacggtg cggcgatgca aatggacgcg agcgaggagc cgtcggcgac ggcggtcgag
      721 acggatgagg taggcgagca gcagcggtcg gaggcggcgg tccctgcgac agcgccgcag
      781 gcgttgccgc tggtggctat cgtcgggcga ccgaacgtgg gcaagtccac gctcttcaac
      841 cggctggtgg gcgagcgccg ctccatcgtc ggcgacgagc cgggcatcac gcgcgaccgc
      901 atctacggcg agacggactg ggccgggcgc cgcttctcga tcgtggacac gggcggcatc
      961 gtgccggacg agacggaggt catccccgcg aacattctgc ggcaggcaga gttcgccatc
     1021 acggaggcgg tcgcgctggt gtgggtggtg gacgtgcgcg cggggttgac gccgctcgac
     1081 gaggagttgg cgcggctgct gcggacgacc gacaagacga tcatcgtcgc ggcgaacaag
     1141 gcggacgcgc tcaagctcga agcgcacgcg ggcgagttcc atcgcttcgg cttcgaggac
     1201 gtggtgccca tctcggcgga gcagggcgac ggcgtggcgg agcttttgga cgtgctggtc
     1261 gagcatcttc cttcgttcga ggagatgggg cttgagttcg cacagacgga agcgcaggtg
     1321 cggagcggcg aggtgcggct cgccatcgtc gggcgaccga acgtgggcaa gtcgtctctg
     1381 ctgaaccgtc tgctcggcga ggagcgcgtc atcgtctcgc cggtcgcggg cacgacgcgc
     1441 gacgccgtcg atacagtgtt gcagcacgag gggaagaatt ttcgtctggt cgatacggcg
     1501 ggcatccggc gcaaggggaa gaccgaggcg atggcggaga agctgtcggt cgtgatggcg
     1561 cggcggagcc ttgagcgcgc ggacgtggcg gtcgtcgtgg tggacgcggt ggacggcgtc
     1621 gccgcgctcg acgcgaacat cgcgggctac gcggtggacg cgggctgctc cgtcatcatc
     1681 gccgtcaaca agtgggacgc gttggcgaac aaggagacgg gcactcccgc cgcgttcgag
     1741 cgcgacctgc gtctgaagat gaagtttctg gagtgggcgc cggtcgtcac gatctccgcg
     1801 ttgaccgggc agcgcgtgga tcgtctcctc cagctcgcgc tgcacgcgaa cgaggcgcgc
     1861 aaccgccgca tccccacttc gcgcttgaac gaattcttcg agcgcgccgt cgccagcccg
     1921 cgcgcgccga ccgcgcccgc gccggtgaag ggcgggcgct cgcgcctgca cgtgcagtac
     1981 gtgacgcagg tcggcgtcag gccgccgcgc ttcgtcgtct tcacggcggg cggtcgcgcg
     2041 gggatacact tttcctacga gcgctacctg caaaaccgtc tgcgcgagga gttcgacttt
     2101 ttcgccacgc ccctcagaat cgtagagcgt caccggcgcg agcgccggaa gccgtgaacg
     2161 tttcttcgcc gcctcatact tgaatcgact tcaaggacaa agacgaggac gcccgatgga
     2221 ggcactcgac gcctcgaatt tacggaagct gttctggcac acgagcgagc cggcctacgt
     2281 cgtggacgcg gcgacgaagt gcgtgctggc cgcgaacccg ccgttcgcgg agctgacgaa
     2341 gaggactccc gacgcgctcg tcggcgcgaa ggaagatgcg ctgctcgcgt tcgaagacgg
     2401 cgaccaggaa ggcgcggcgg gcgagtgggc gcggctgctt gcgggcgcgg acgggggcgg
     2461 gcacgaaggc gacggcgtgc gcgtgcggct cgaaagtttc ccgtgcgtgt gggaagggaa
     2521 ggcggcgtgc ctctacttcg tccggcgcgc ggacgcgggc ggcgacggcc ttcgcgtcga
     2581 agccgaaggc gaccgcgcgc gggcgttgct cgattacctg cacgaggcga ccgaccagct
     2641 cgaagtcatc aaccgcgtcg tcgccgcggt caacgcgggg agcacgatcg aagatgtctt
     2701 ccgcccggcg agcgagcaga tacgcacgct cgtcccgttc gaccgcgcgt ccatcgcgct
     2761 gctcgacgca gggagcagcc tgcgcgtctt cgcgctctcg ggagaggccg gctcgctcgc
     2821 cgtcggcgcg accgcgccct tgcgcggctc ggtcacggag acggcgctcg ccgggcggca
     2881 gatggtctcc atccccgaac tcgcggagga gacgcgcttc aacgtctacg aagacctcag
     2941 gcgcgagggc ttccgctccg ccgtctgcgt cccgctcttc tcgatgagcc gcgccgtcgg
     3001 cagcctgaac ctcacgagcc gcacgcccga cgcctacaag cgcagacacc tcgccgcgct
     3061 cgaacgcctc gcgccgccgt tcgccatcgc catcgagaag gtgctgctgc tggaagcgag
     3121 ccgccaccgc accgaggagc tgcgcgggct gttcgaaatc tcgcgcgcct tctcgacgct
     3181 caccgacact tcggagatca gcagccgtct ggcgcgcgcc atcgccaagc tcgtcggcgg
     3241 gacgatgtgc ctgatcgcga cctacgaccg ccgccgcaac gcagtgcgcg ccgagtcgcc
     3301 cggctaccgc acgccgcccg aactcgtccg cgagttccag ttcacgctcg accgaggcaa
     3361 ccaccacgcg ccgctctacc aggtcggcga ggcgttcctc tcgaacgacc ctgcgcgcga
     3421 cgcgcgcctc aaccgccggt tcgtcgagcg ctggggcgtc cacaacgtcc tcagcgtgcc
     3481 gatgcggatc aagaaggagc tgatcggctt catctacgtc gccaaccgcg aggggagctt
     3541 cagcgagcgc gacctgcggc tgctggaaat cttcgccgcg caggccgccg aaaccatcgt
     3601 caacgcgcgc ctcttcctga ccatccaggc gcaggccgag cgcgaggccg tcgtcaaccg
     3661 cctgctgctc tctttgcagc gcagcggcga gccgaaggag aaggtgagcg ccgtcatcga
     3721 gcgcatcggc gaggtcttgg aactcgaccg ctgcgtcgcg gtgctcttcg ccgacggcga
     3781 ccacgacgac tactacggcg agtggtgcgc cgacggcgtc gagccggtca cggactggat
     3841 ggacatgcgc gagcgctcgc ccatccgcta cgcgctgcgc accgcgcgcc gcccgctcta
     3901 cgccacggac gtgcgcgagc acccgctcgc ccccggcctc gaagacctca tcgagcgctc
     3961 gaatttgaag agcctgctgg tcgtgccgat catccatcag ggccgcgtca tcggctcgat
     4021 cagcggccac cagacgcgca ccttccgcaa ctggaacgag gacgacatcg acctgctcgt
     4081 ggccgtcgcc acgcacgtcg gcgcgacgct cgaaaacgcg cggctcatcg ccgagctgcg
     4141 cgaggccaac cgcctgaagg acgagtttct ggcgacgctc tcgcacgagc tgcggacgcc
     4201 tttgacggcc atcaccggct gggtcgagat tctcgccgag gcgcaccggg cgcacaaaga
     4261 cgaagacgtg gccgacggca tcaacgccat cagcgcctcg gccacctcgc tcgcgcagct
     4321 catctccgac ctgctcgacc tgtcgcgcat ccagcgcggc gtgctccgcc tcgaacggca
     4381 gttcttcgac gtgaatcagg tggtcgcggg cgccgcacgc aacgtccggc aggccgcgct
     4441 cgcgcgccgc ctcgacttgc ggctggagct ggcggagaat gtgcccacga ccatcgccga
     4501 cacgcagcgc gtgcagcagg tgttgtggaa ccttctgatg aacgccatca agttcacgcc
     4561 gcagggcggg gcggtgacgg tcaggacgcg gctcgtcgaa gactggccgc agggcggcga
     4621 aggcgaaggg gccgagcggg acgcgcgcgg cctcgtccgg tgggtcgagg tcgaggtcga
     4681 agacacgggc gaaggcatcc ccgaggactt catgccctac atctgggatc gcttccgtca
     4741 ggccgacggc tcggccacgc gccgccacgg cggactcggc atcggcctct cgctcgtcaa
     4801 ggaactcatc gaggcgcacg gcgggcaggt caacgcccac agccagcagc gcggcgcgcg
     4861 cttcaacgtc cgcctgcccg tcgtcagcgt cgaaggctgg ctcgcgcacg agccgcgctt
     4921 cgaacgcgac gagtacgacg gccagagatg aacatgaaac gtgcgcgccg acgcgccgcg
     4981 tggcggcggc tg
//
LOCUS       DBNGOH010000607         3663 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2289528, whole genome shotgun sequence.
ACCESSION   DBNGOH010000607 DBNGOH010000000
VERSION     DBNGOH010000607.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3663)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3663)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3663
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2289528"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(34..963)
                     /locus_tag="AC159P_11785"
     CDS             complement(34..963)
                     /locus_tag="AC159P_11785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010038675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HIR3696171.1"
                     /translation="MKLAQFKTKASSRRRLGILVEEMLIDVAALARASREAGGRVSDW
                     LPGAADTLDVIKRGPEALGEIAALVGDERARALFADEGVAWATEAVEFLPPVCPGKML
                     AIGRNYVDHAIEGGAEPPAAPLVFMKLPNALSAHEAPIVLPAISTQVDYEAELAVVIG
                     RRAKRVREREALEYVFGYAPFNDVSARDLQFGDGQWVRGKGLDGFAPLGPYITTRDEV
                     PDAHALKIEGRLNGAVMQSSVTSKMVFKIPYLVSYISQGITLEPGDVIATGTPEGVGI
                     FRNPPVLLKAGDVFEVVIEQLGTLRNAVVGAEG"
     gene            complement(1084..2028)
                     /locus_tag="AC159P_11790"
     CDS             complement(1084..2028)
                     /locus_tag="AC159P_11790"
                     /inference="COORDINATES: protein motif:HMM:NF014684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3696172.1"
                     /translation="MSDFVKRFLPFVSLLALCVLIAVLEPKFLSAGNLAGVARQTAVI
                     TIIAMGMTMVMVAGGIDLSVGSVMALAGVAGASVLVAGAPVVAGVAACVAAGAACGLA
                     NGAAISALRIPPFIVTLGAMGIYRGVALLWTDGKAVVGLPSSFGYLAEGQLFGVVPVP
                     LVVVVCVALATHFVLASTRPGRYCYAIGSNVEAARYAGVRVSRYQIMFYVILGALAGL
                     AGAIESARLVTGQPTAGEGYELRVIAAVVIGGGSLSGGQGTVTGTIIGALIMGVLSNG
                     ANLLGITAFTQQIVVGAVIVLAVTFDEFQRRRLETAIS"
     gene            complement(2035..3612)
                     /locus_tag="AC159P_11795"
     CDS             complement(2035..3612)
                     /locus_tag="AC159P_11795"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017982088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HIR3696173.1"
                     /translation="MSSNSFALEMRGITKSFAGNTVLAGVDLAAAAGEVHALVGENGA
                     GKSTLMKVLAGVHQPDAGEVSVDGRAVRFARPADALAHGVAMIYQELSLAPHLTVAEN
                     IFLGREPLSFAPLAVVNRREMNERAARLLAGYGFRLDARARVGRLSAADRQLVEIARA
                     TAEARRVIVMDEPTSSLTAHEVEELFRLIRDLKARGLAVIYISHRLEELERIADRLTI
                     LRDGRAVYAGKWGELSRDEIIRHMAGRELKEIFPPRSAEVGGALLRVERLTRAGKFEG
                     VSFEARAGEVLGLAGLAGAGRTELVEAVFGAQRADSGEIHLDGARVAEARPDRSVARG
                     LGLLTEDRKRTGLCLNLELGKNLTLANVRALLKNWRLERGRERAAAREYIEKLHIRPP
                     DPEKTVGRLSGGNQQKVLLARWLFAGSRVFLLDEPTRGVDVAARAEIYRAINKLAEGG
                     AAVVMVSSDLPELLGMADRILVMRRGRLVAELNAGETTQEEILRHAAVEEDERSEAET
                     PDARPRVIPEVQVVGKE"
BASE COUNT          600 a         1371 c         1139 g          553 t
ORIGIN      
        1 gccgctttca agcgcgccgc tcgaatttcg ccctcacccc tcggcgccga ccacggcgtt
       61 gcgcagcgtg ccgagctgtt cgatgacgac ctcgaacacg tcgcccgcct tcaggagcac
      121 gggcgggttg cggaagatgc cgacgccttc gggcgtgccg gtggcgatca cgtcgccggg
      181 ttcgagcgtg atgccctgcg agatgtacga gacgaggtaa ggaattttga agaccatctt
      241 cgacgtgacg gacgactgca tcaccgcgcc gttcagtcgg ccttcgatct tcagcgcgtg
      301 cgcgtccggc acctcgtcgc gcgtggtgat gtaggggccg agcggcgcga agccgtcgag
      361 gcccttgccg cgcacccact ggccgtcgcc gaactgcaaa tcgcgcgcgc tcacgtcgtt
      421 gaaaggcgcg tagccgaaaa catattcgag cgcttcccgt tcgcgcacac gcttcgcgcg
      481 ccgaccgatg acgaccgcca gctcggcctc gtaatcgacc tgcgtgctga tcgcgggcag
      541 cactatcggc gcctcgtgcg cgctcagcgc gttcggcagc ttcatgaaga cgagcggcgc
      601 ggcgggcggc tccgccccgc cctcgatggc gtgatccacg tagttgcggc caatcgccag
      661 catcttgccg gggcagaccg gcggcagaaa ctccaccgcc tccgtcgccc acgccacgcc
      721 ctcgtccgcg aagagcgcgc gcgcacgctc gtccccgacg agcgccgcga tctcgcccaa
      781 agcttcaggc ccgcgcttga taacgtcgag cgtgtccgcc gcgccgggca gccagtccga
      841 cacgcgcccc cccgcctccc tcgacgcacg cgccagcgcc gccacgtcaa tcaacatctc
      901 ttctacgaga atgccgagtc gccgcctgct cgacgctttg gttttgaact gagccagttt
      961 catccgagtt tacctctgct gcgatttcgg atttcggatt tcggattggc gtgctgctca
     1021 agctcgacgc ctgattttga acgctcaact ttcgtcactc ggataatccg aaatccgaaa
     1081 tcgctaggat attgccgttt cgagccgccg ccgctggaac tcgtcgaacg tgacggcgag
     1141 gacgatcacc gcgccgacga cgatctgctg cgtgaaggcg gtgatgccga gcaggttcgc
     1201 gccgttcgag agcacgccca tgatgagcgc gccgatgatc gtgcccgtca cggtgccctg
     1261 cccgccgctc aaagagccgc cgccgatgac gaccgccgcg atgacgcgca gctcatagcc
     1321 ctcgcccgcc gtcggctgcc ccgtcacgag ccgcgccgac tcgatggcgc ccgcgagtcc
     1381 cgccagcgcg ccgaggatca cgtagaacat gatctggtaa cgcgacacgc gcacgcccgc
     1441 gtagcgcgct gcctcgacgt tgctgccgat ggcgtagcag tagcgaccgg gccgcgtgct
     1501 cgcgagcacg aagtgcgtcg ccagcgcgac gcagacgacc acgacgagcg gcaccggcac
     1561 cacgccgaag agttgcccct cggccagata gccgaagctc gaaggcagac cgaccacggc
     1621 cttgccgtcc gtccacagca gcgccacgcc gcgataaatc cccatcgcgc cgagcgtcac
     1681 gatgaacggc ggaatccgca gcgccgagat ggccgccccg ttcgcaagtc cgcacgccgc
     1741 gcccgccgcg acgcacgccg cgacgcctgc gacgaccggc gcccccgcca ccaacacgga
     1801 cgccccggcc acccccgcca gcgccatcac cgacccgacc gacaaatcga tcccgcccgc
     1861 caccatcacc atcgtcatcc ccatcgcgat gatggtgatg acggcggtct gccgcgccac
     1921 gcccgccaga ttccccgccg acagaaactt cggctccagc accgcgatca gcacgcacag
     1981 cgcgagcagg ctgacgaagg gcagaaagcg tttgacgaaa tcggacatga ccgtctactc
     2041 cttgccgacg acttgaactt cgggtatcac gcgcgggcgc gcgtccggcg tttcggcctc
     2101 cgagcgctcg tcctcttcga ccgcggcgtg tctcaggatt tcttcctgcg tcgtctctcc
     2161 ggcgttcagc tccgcgacca gccgaccgcg gcgcatgacg aggatgcggt cggccatgcc
     2221 gagcagttcg ggcaagtccg aagagaccat gacgacggcg gcgccgcctt cggcgagctt
     2281 gttgatcgcg cggtaaatct ccgcgcgcgc ggccacgtcc acgccgcgcg tcggctcgtc
     2341 cagcaggaag acccgcgacc cggcgaagag ccaccgcgcg agcagaacct tttgctggtt
     2401 gccgccgctc aaacgcccga cggtcttctc ggggtcgggc gggcggatgt gcagcttctc
     2461 gatgtactcg cgcgccgccg cgcgctcgcg cccgcgttcg agccgccagt tcttcagcag
     2521 ggcgcgcacg ttggcgagcg tcaggttttt tccgagttcg aggttgaggc acagccccgt
     2581 gcgcttgcgg tcttcggtga gcagccccag cccgcgcgcg accgagcggt cgggccgcgc
     2641 ctccgcgacg cgcgcgccgt ccagatgaat ctcgccgctg tccgcgcgct gcgcgccgaa
     2701 gacggcctcg accagttccg tgcggcccgc gcccgcgagg cccgcgaggc ccaacacttc
     2761 gcccgcgcgc gcctcgaagc tcacgccctc gaacttgccc gcgcgcgtca gacgctcgac
     2821 gcgcagcagc gccccgccca cctccgcaga gcgcggcggg aaaatctcct tcagctcgcg
     2881 cccggccatg tggcgaatga tttcgtcgcg cgaaagctct ccccacttcc ctgcgtagac
     2941 cgcgcgcccg tcgcgcagga tggtcaggcg gtcggcgatg cgttcgagtt cttccagccg
     3001 gtgcgagatg tagatgacgg cgaggccgcg cgccttgagg tcacgaatca ggcggaacag
     3061 ctcttccact tcgtgcgccg tcagcgaaga ggtcggctcg tccatcacga tcacgcgccg
     3121 cgcctccgcg gtcgcgcgcg cgatctcgac cagttggcgg tcggcggcgc tcagacgccc
     3181 gacccgcgcc cgcgcgtcga gccgaaagcc gtagcccgcg agcagccgtg ccgcgcgctc
     3241 gttcatctcg cgccggttga cgaccgcgag cggcgcgaag ctcagaggct cgcgccccag
     3301 aaagatgttc tcggccaccg tcaggtgcgg cgccagcgaa agctcctgat agatcatcgc
     3361 gacgccgtgc gccagcgcgt ccgccggtcg cgcgaagcgc accgcccgac cgtcaaccga
     3421 cacctcgccg gcgtccggct ggtgcacgcc cgccagcacc ttcatcaacg tcgatttgcc
     3481 cgcgccgttc tcgcccacca gcgcgtgcac ctcgcccgcc gccgccgcca gatcgacccc
     3541 ggccagcacc gtgttccccg cgaacgactt cgtgatgccg cgcatctcga gcgcgaacga
     3601 gttggaagac ataagagaga aatgatgaat gcagaatgcg gaatgcggaa tgagattcac
     3661 gct
//
LOCUS       DBNGOH010000608         3504 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4489067, whole genome shotgun sequence.
ACCESSION   DBNGOH010000608 DBNGOH010000000
VERSION     DBNGOH010000608.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3504)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3504)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3504
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4489067"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="AC159P_11800"
     CDS             complement(<1..242)
                     /locus_tag="AC159P_11800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HIR3696174.1"
                     /translation="MKPFKIRNVEISPPLVLSPMAGVTDVPFRALLKRRGGIGLTVSE
                     FISVEGLTRNNPKSRRQMRFFDYERPFAVQIFGGQPL"
     gene            complement(363..878)
                     /locus_tag="AC159P_11805"
     CDS             complement(363..878)
                     /locus_tag="AC159P_11805"
                     /inference="COORDINATES: protein motif:HMM:NF027592.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4920 domain-containing protein"
                     /protein_id="HIR3696175.1"
                     /translation="MKTFLVTLLLVSALAVSAFARQTDKPAKHDAHKAHKADAAGEVV
                     KRGTPIGDKSPAVKFADVFKEPQKFAGKSVVIEGVVERVCKAEGCWMQIAPEAGAAET
                     VRVTFDHKFSVPKDADKMKFRAEGEFSVKVLSKEHVEHLVKEDGAKIKTNPDGTANEV
                     SFLATGVELWK"
     gene            1046..1546
                     /locus_tag="AC159P_11810"
     CDS             1046..1546
                     /locus_tag="AC159P_11810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696176.1"
                     /translation="MKTKLIQSVGIAWTLLYAALVVALYATAPRSFSEVATQASVVAG
                     TYEIDRARFDAARELFLREQYAAARDEWSRADPARRDARTQFYIAYAFYRQGWGRVYN
                     DDALFRQGLETANLALGLAPEGALAVEDPELKLRTAAELKAELEQGLQTTADDLNPFK
                     VFRERK"
     gene            1572..1736
                     /locus_tag="AC159P_11815"
     CDS             1572..1736
                     /locus_tag="AC159P_11815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696177.1"
                     /translation="MSGTLTNSRVPEDRVRHERRFIGLTLGFVLATVVIFVATVCGVL
                     VVWVLLFFLF"
     gene            1742..2515
                     /locus_tag="AC159P_11820"
     CDS             1742..2515
                     /locus_tag="AC159P_11820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696178.1"
                     /translation="MSHRTRNRALARFVLLPTIFLTVALLGGLRLSAETGALVFVAPP
                     LVALVLALMLMLLFARGGAIRFGRWLSADNPTLSNVSHALTLAALFFASAQSFNSVLP
                     ERGLLRWMLSCFFLWTLWQNQFATFDPRRLLRSLAALFGTAFVVKHLLLTSLYAPDGG
                     WLKRLAGVVLEGVTLGTLDGQAFAPATGYVSFFTLALYVGGLALLPPAPEDDQVEDED
                     AEEVTLVSKRLTAAERDKLAGEAGVIEGEAVEAPLRDKA"
     gene            complement(2609..>3504)
                     /locus_tag="AC159P_11825"
     CDS             complement(2609..>3504)
                     /locus_tag="AC159P_11825"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013618.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008933 - peptidoglycan lytic
                     transglycosylase activity [Evidence IEA];
                     GO_function: GO:0016837 - carbon-oxygen lyase activity,
                     acting on polysaccharides [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MltF family protein"
                     /protein_id="HIR3696179.1"
                     /translation="ELEDDISGDIDVVEWTGKIGDEALAQKVARGEVEFTVMQSNLAE
                     LKEAEFTNLKVRPIVGRVHKVSWAVRKNSPELLAHLDRWVEEKKNTALFNQLYKKYFI
                     DRQRHRERAASEYLASKTGKLCQYDELLKRYAPELEWDWRLLASQAYQESRFNPTARS
                     WAGATGLLQLMPRTAQQFGVTDPLDPEDNLRGGVRFLQWLKAYWAKRIPDEGERLKFV
                     LASYNTGQGHVEDAQRLAEKHGGDPQRWEDVSYWLLQKSKQQFSTDPVVKFGFCRGIE
                     PVTYVSLIMDRFDHYRQFVAD"
BASE COUNT          530 a         1173 c         1148 g          653 t
ORIGIN      
        1 aggggctggc cgccgaaaat ctgcacggcg aacgggcgct cgtagtcgaa gaagcgcatc
       61 tgccgcctcg acttcgggtt gttgcgcgtc agcccctcga ccgagatgaa ctccgagacg
      121 gtcaggccga tgccgccgcg ccgcttcaag agcgcgcgga acggcacgtc cgtcacgccc
      181 gccatcggcg agagcacgag cggcggactg atctcgacgt tacgaatctt gaacggcttc
      241 ataaaaacct tggggaaaag ggtaaaaggg gaaagggaga aaagggagaa aagaatcgcc
      301 ccgtccaacg tcttcggttt tccttttctc cctttcccct tttacccttt tcccctcttc
      361 ctctacttcc acagctcgac gccggtggcg aggaagctga cttcgttggc cgtgccgtcg
      421 gggttggtct tgatcttcgc gccgtcttcc ttcaccagat gctcgacgtg ctccttgctc
      481 agaaccttga cggagaactc gccctcggcg cggaacttca tcttgtccgc gtccttcggc
      541 acgctgaact tgtggtcgaa cgtgacgcgg acggtctccg ccgccccggc ctcgggcgcg
      601 atctgcatcc agcagccctc ggccttgcag acgcgctcga cgacgccctc gatcaccacc
      661 gacttgcccg cgaacttctg cggctctttg aacacgtcgg cgaacttgac ggcgggcgac
      721 ttatcgccga tgggcgtgcc gcgcttgacg acttccccgg cagcgtccgc cttgtgggct
      781 ttgtgcgcgt cgtgcttggc gggcttgtcg gtctgccgcg cgaaggccga gacggcgagc
      841 gcggagacga ggaggagcgt gacgagaaat gttttcatga cgagaatgcg ttatcctttc
      901 acacgagatt ctacgggccg gaactttttt ttccgaggcg tccggattat agcaacgcgg
      961 cgcgggccgc gccaaagagg cgaccgcgac agacgggacg gaacggcgcg cttgatgcgt
     1021 tgcagacgaa atcgattcga ctcaagtgaa gacgaaactg atccagagtg tcggcatcgc
     1081 gtggacgctg ctctatgcgg cgctcgtcgt cgcgctctac gcgaccgcgc cgcgcagttt
     1141 cagcgaggtg gcgacgcagg cgagcgtcgt cgccggcacc tacgagatcg accgcgcgcg
     1201 cttcgacgcg gcgcgcgagt tgttcctccg cgagcagtat gcggcggccc gcgacgagtg
     1261 gtcgcgcgca gacccggcgc ggcgcgacgc gcgcacgcag ttctacatcg cctacgcttt
     1321 ttaccggcag ggctggggcc gcgtctacaa cgacgacgcg ctcttccggc agggactcga
     1381 aacggcgaac ctcgcgctcg gcctcgcgcc cgagggcgca ctcgccgtcg aagacccgga
     1441 gctgaagctg cgcaccgccg ccgagctgaa ggcagagctt gagcaggggc ttcagacgac
     1501 ggcggacgac ctcaacccgt tcaaagtttt ccgcgagcgg aagtgaccgc gagcagaaag
     1561 ggcggcgacg gatgtccggc acgctgacca actcccgcgt ccccgaagac cgtgtgcgcc
     1621 acgagcgccg gttcatcggc ctgacgctcg gcttcgtcct cgcgacggta gtcatcttcg
     1681 tcgccacggt ttgcggcgtg ctcgtcgttt gggtgctcct tttcttcctc ttttgaccgg
     1741 catgagtcac cgcacacgca accgcgcgct cgcgcgtttc gtcctgctgc cgaccatctt
     1801 cctgacggtc gcgctgctcg gcggcctgcg cttgagcgca gagaccggcg cgctcgtctt
     1861 cgtcgcgccg ccgctcgtcg cgctcgtgct ggccctgatg ctcatgctgc tcttcgcgcg
     1921 cggcggcgcg atcaggttcg ggcgctggct ctcggctgac aacccgacgc tctcgaacgt
     1981 ctcgcacgcg ctcacgctcg cggcgctctt cttcgcctcg gcgcagagct tcaactcggt
     2041 gctccccgag cggggcctgc tgcgctggat gctctcgtgc ttcttcctgt ggacgctctg
     2101 gcagaaccag ttcgccacgt tcgacccgcg gcgcctgctg cgcagcctcg ccgcgctctt
     2161 cggcaccgcg ttcgtcgtca aacacctgct cctgacttcg ctctacgcgc cggacggcgg
     2221 ctggctgaag cggctggcgg gcgtcgtgct cgaaggcgtc acgctcggca cgctcgacgg
     2281 gcaggcgttc gcgcccgcca ccggctacgt ctccttcttc acgctcgcgc tctacgtcgg
     2341 cggcctcgcg ctgctgccgc ccgcgcccga agacgatcag gtggaagacg aggacgcgga
     2401 ggaagtgacg ctggtgtcga agcgtctgac ggctgccgag agagataagc tggcgggcga
     2461 agccggcgtg atcgagggcg aagccgtcga ggccccgctg cgagacaagg cgtgagcggt
     2521 cgtcgggagg tgaagcgcgg ggggccgcgt gaaaagtctt cggcgggact tttcgcgcgg
     2581 cccccggagt cttcgcgcgc gggccgcgtc agtcggcgac gaactggcgg tagtggtcga
     2641 acctgtccat gatgaggctg acgtaggtga ccggctcgat gccgcggcag aagccgaact
     2701 tgacgacggg gtcggtcgag aactgctgct tcgacttttg caggagccag taggacacgt
     2761 cctcccagcg ttgcgggtcg ccgccgtgct tttcggcgag gcgctgcgcg tcctcgacat
     2821 ggccctgccc cgtgttgtaa gaggcgagga cgaacttcag ccgctctccc tcgtcgggga
     2881 tgcgcttggc ccagtaggct ttgagccatt gcaggaacct gacgccgccg cgcaggttgt
     2941 cttccgggtc gagcgggtcg gtcacgccga actgctgcgc ggtgcggggc atcagttgca
     3001 gcagccccgt cgcgcccgcc caagagcgcg ccgtcgggtt gaagcgcgac tcctgatacg
     3061 cctgcgaggc cagcagccgc cagtcccatt ccagctcggg cgcgtaacgc ttcagcagct
     3121 cgtcgtactg gcagagcttg ccggtcttcg aagccaggta ctcgctcgcc gcgcgctcgc
     3181 ggtggcgctg gcggtcgatg aagtatttct tgtagagctg gttgaagagc gccgtgttct
     3241 tcttctcctc gacccagcgg tcgaggtgcg cgagcagctc cggcgagttc ttccgcacgg
     3301 cccaactgac cttgtgcacg cgcccgacga tgggccgcac cttgaggttg gtgaactccg
     3361 cctccttgag ttcggcgagg ttgctctgca tcacggtgaa ctcgacctcg ccgcgcgcga
     3421 ccttctgcgc cagcgcctcg tcgccgatct tccccgtcca ctcgacgacg tcgatgtcgc
     3481 ccgagatgtc gtcctcaagc tcga
//
LOCUS       DBNGOH010000609         3458 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3572959, whole genome shotgun sequence.
ACCESSION   DBNGOH010000609 DBNGOH010000000
VERSION     DBNGOH010000609.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3458)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3458)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3458
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3572959"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(109..1422)
                     /locus_tag="AC159P_11830"
     CDS             complement(109..1422)
                     /locus_tag="AC159P_11830"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HIR3696180.1"
                     /translation="MRLLITNGYVVDPSQGVNAGMDVFIEDGRVAALIGRGEAKPEGC
                     EVFDATGLVVAPGFIDLHTHLREPGQEFKETIATGAAAAVAGGWASVCAMPNTDPVND
                     NPAVTSSIIEQARLARLANVFPVGAITKGSQGAELAEMGEMKRAGIVAVTDDGRPVPS
                     TGLMRRAMEYARGFDLTVMDHCEDRSLSAGGVMHEGEWSLRLGLRGAPALAEESDAAR
                     DCALAELTGARVHLAHVSTRGAVEAVRRAKEKGINVTCEVAPHHFTLTDEACEAYDTN
                     TKMNPPLRSREHLEAVVEAVADGTIDAIATDHAPHHSDEKALEFDHAPFGITGLETAV
                     GLALDRLVHGGHLDLARLVEMCSVNPARIFRLAGRGTLRTGAHGDVTILDTDLKWTFD
                     AARSRSKSRNTPFDGWTFQGAAVATIVGGRVVYRREQAAAGGSRQ"
     gene            complement(1534..2559)
                     /locus_tag="AC159P_11835"
     CDS             complement(1534..2559)
                     /locus_tag="AC159P_11835"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HIR3696181.1"
                     /translation="MKTGLLHKHLLGIRELSAAEVVHVLDTAESFREISEREIKKVPA
                     LRGKTVINLFFEASTRTRTSFEIAGKRLSADVINISSSSSSVTKGETLLDTARNLEAM
                     APDLIVIRHPAAGAPHQLARVCRASVVNAGDGAHEHPTQALLDALTIRQRKGKIEGLK
                     VAVLGDILHSRVARSNAHLLTKLGASVALAGPGTLAPREFASLVEGGGLRVERRIEDA
                     IEGADVVMVLRIQRERQDSAFLPSMREYAVHYGLNRKRLELAAPDAIVMHPGPMNRGI
                     EIASDVADSARSLILDQVANGLAVRMAVLYLLAGGAPSGETAPPEPLTQAKRETGATP
                     ETFKATA"
     gene            complement(2740..3303)
                     /gene="pyrR"
                     /locus_tag="AC159P_11840"
     CDS             complement(2740..3303)
                     /gene="pyrR"
                     /locus_tag="AC159P_11840"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014098590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HIR3696182.1"
                     /translation="MDFVEKARIMDGMRINRALARLASEIVEENHGAGHLYLVGIQRR
                     GVPLANRLADKIADLEGERPPVGVLDITLYRDDLSQVGANPVVNRTDLPVDVEGRNVL
                     LIDDVLYTGRTIRAALDQLIDFGRPRRVQLAVLVDRGKEHRELPIQADFVGKFVPTKK
                     SEIIKVMLDDIDGEEGVAIVERPSNEA"
BASE COUNT          527 a         1211 c         1120 g          600 t
ORIGIN      
        1 aaggagcttg ggactttacc tcccaagcct cttctctctt cagtctcctg tcttatgttt
       61 taactgccta ccgcctactg cctactgcct actgcctact gccactgcct actgcctact
      121 tcctccggcg gctgcctgct ctctcctgta gacgacgcga ccgccgacga tggtggcgac
      181 cgccgcgccc tgaaacgtcc agccgtcgaa gggcgtgttg cggctcttcg accgggagcg
      241 cgcggcgtcg aacgtccact tcaagtccgt gtcgagaatc gtcacgtcgc cgtgcgcgcc
      301 cgtgcggagc gtgccgcgcc ccgcgagccg gaagatgcgc gcggggttga cggagcacat
      361 ctcgacgagg cgcgccagat cgaggtggcc gccgtgcacg aggcggtcga gcgcgaggcc
      421 gaccgccgtc tcaaggccgg tgatgccgaa gggcgcgtgg tcgaattcga gcgccttctc
      481 gtccgagtgg tgcggcgcgt ggtctgtggc gatggcgtcg atcgtcccgt cggcgacggc
      541 ctcgacgacc gcttccagat gctcgcggct ccgcagcggc gggttcatct tcgtgttcgt
      601 gtcgtaagcc tcgcacgcct cgtccgtcag cgtgaagtgg tgcggcgcga cttcgcaggt
      661 gacattgatg cccttctcct tcgcgcgccg gacggcttcg acggcgccgc gtgtcgagac
      721 gtgtgcgaga tgtacgcgcg cgcccgtcaa ctccgcgagc gcgcagtcgc gcgccgcgtc
      781 cgactcttcg gcgagcgcgg gcgcgccgcg cagacccaga cgcagagacc actcgccctc
      841 gtgcatcacg ccgcccgccg agagcgagcg gtcttcgcag tgatccatca cggtcaggtc
      901 gaagccgcgc gcgtactcca tcgcgcgccg catcagtccc gtcgaaggga cgggccgccc
      961 gtcgtccgtc acggcgacga tcccggcgcg cttcatctcg cccatctcgg ccagctccgc
     1021 gccctgcgaa cccttcgtga tcgcgccgac ggggaagacg ttggcgagac gagcgagacg
     1081 cgcctgctcg atgatcgagc tcgtcaccgc cgggttgtcg ttgacggggt ccgtgttcgg
     1141 catcgcgcac acgctcgccc acccgcccgc gacggcggca gccgcgcccg tggcaatcgt
     1201 ctccttgaac tcctgccccg gctcgcgcag gtgcgtgtgc aggtcgatga agccgggcgc
     1261 gacgacgagg cccgtcgcgt cgaagacctc gcagccctcg ggcttcgcct cgccgcgacc
     1321 gatcagcgcg gccacgcgcc cgtcttcgat gaacacgtcc atgccggcgt tgacgccctg
     1381 cgaagggtca accacgtatc cgttcgtgat gagcagtctc ataatcagtg aacagtaagc
     1441 ggtaagcagt gagcagtaaa agtcggttat tacgaaccgg cagtgagcgt tcgtgttcga
     1501 actgcttact gctcacagct cactgctcac gtgttacgcg gtggccttga aggtttccgg
     1561 cgtcgccccc gtctcccgct tcgcctgcgt caggggttcg ggcggcgccg tctcgcccga
     1621 gggtgcgccg cccgccagca ggtagagcac ggccatgcgg acggcgaggc cgttggcgac
     1681 ctgatcgagg atgagcgagc gcgcggagtc ggccacgtcc gacgcgatct cgatgccgcg
     1741 gttcatcggg ccggggtgca tgacgatggc gtcgggcgcc gccagttcga gccgcttccg
     1801 gttcagcccg tagtggacgg cgtactcgcg catcgaaggg aggaaggccg agtcctgccg
     1861 ctcgcgctgg atgcgcaaga ccatcaccac gtcggcgccc tcgatcgcgt cctcgatcct
     1921 ccgttcgacg cgaaggccgc cgccctcgac cagcgacgcg aactcgcgcg gcgcgagcgt
     1981 gcccggcccc gcgagcgcga cgctcgcgcc gagcttcgtc aacaggtgcg cgttcgagcg
     2041 cgccacgcgc gagtgcagga tgtctccgag caccgcgacc ttcagcccct cgattttgcc
     2101 tttgcgctgc cggatggtga gcgcgtcgag caaagcctgc gtcgggtgct cgtgcgcgcc
     2161 gtcgcccgcg ttgacgaccg acgcgcggca gacgcgcgcg agctggtgcg gcgcgcccgc
     2221 cgcggggtgg cggatcacga tcaggtcggg cgccatcgct tcgaggttgc gcgccgtgtc
     2281 gagcagcgtc tcgcccttcg tcaccgagga cgaagaggag ctgatgttga tgacatcagc
     2341 cgacaggcgc ttgcccgcga tctcgaagga cgtgcgcgtc cgcgtcgaag cctcgaagaa
     2401 gaggttgatg acggtcttgc cgcgcagcgc cggcaccttc ttgatctcgc gctccgagat
     2461 ttcccggaac gactccgccg tgtcgaggac gtgcacgacc tccgccgccg aaagctcgcg
     2521 gatgccgagc agatgtttat gcagcagccc tgtcttcatc catcacccaa cggaaggggc
     2581 gaaaagggga aaagaagaag aggcgaaaag gacgacggtt gaaggcgtgt gtgcccaacc
     2641 tgcttgcctt tccccttttt cccgcttccc cttttcgccc cttccttcat tcgtctcgtc
     2701 ttcacgcaaa aaaaaggcgg ccatcgctgg ccgccgcgct tacgcttcgt tcgagggtcg
     2761 ctcgacgatg gcgaccccct cctccccgtc gatgtcgtcg agcatcacct tgatgatctc
     2821 gctcttcttc gtcggcacga acttcccgac gaagtcggcc tggatgggaa gctcccggtg
     2881 ctccttgccc ctatcgacga ggacggcgag ctgcacgcgc cgcggcctgc cgaagtcgat
     2941 gagctggtcg agcgccgcgc ggatcgtccg gcccgtgtag agcacgtcgt cgatcagcag
     3001 cacgttgcgc ccctccacgt ccaccggcaa gtccgtccgg ttgaccaccg ggttcgcgcc
     3061 gacctgcgac agatcgtccc ggtacaaagt gatgtcgagc acgcccacgg ggggccgctc
     3121 gccttcgagg tcggcgatct tgtccgccag ccggttcgcc agcggcacgc cccgccgctg
     3181 gatgcccacc agatacagat gccccgcgcc gtggttctcc tcgacgatct ccgacgccag
     3241 ccgcgccagc gcacggttaa tccgcatccc atccatgatg cgagcttttt ccacgaagtc
     3301 catcgcgcgg gatactaaca cagacgcgcg gacggatgga agatgacgac tgcgaccgcc
     3361 cgcccggcga ggcagggtcg cagtaactct tcgacatctg gccgggaaaa cttgcggacg
     3421 cgcgcgcggc ggacaggggt gtattgcgcc ccgccttc
//
LOCUS       DBNGOH010000610         3259 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2474745, whole genome shotgun sequence.
ACCESSION   DBNGOH010000610 DBNGOH010000000
VERSION     DBNGOH010000610.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3259)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3259)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3259
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2474745"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..483
                     /locus_tag="AC159P_11845"
     CDS             <1..483
                     /locus_tag="AC159P_11845"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF051395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlgC family sugar phosphate
                     nucleotidyltransferase"
                     /protein_id="HIR3696183.1"
                     /translation="NYSFEYGLFPDLLRREESFHAYVPRDAYWIDIGTPARYLQVHQD
                     LLAGRVRGVQVDRPAVDAYREGGAEIDDLSLVGADCVVKPGAQIVNSVLGEGCHVEEK
                     ASVVNSVVWPHTRIAAGAHVAGAVIGRGCHVGRATHIDGAVVGDKSSLTDYTQLGGGL
                     "
     gene            483..1961
                     /locus_tag="AC159P_11850"
     CDS             483..1961
                     /locus_tag="AC159P_11850"
                     /inference="COORDINATES: protein motif:HMM:NF014877.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase/phosphomannomutase family
                     protein"
                     /protein_id="HIR3696184.1"
                     /translation="MTGRERPARIRFGTDGWRALIADEFTFDNLERVAQAYADFLLSE
                     LAMHTTVDESQHVASHLAETLRVVVGYDRRFLSEQFARRAAEVLSGNGLAVSLFERDV
                     PTPLVSWAVREGGAAGGVVITASHNPAPFNGFKIKAPWGGSATEQTTLAVEALVDAHA
                     QRRATELPASVHEGLGEAAESYRRQVASFVDLERIRAARARVVVDPMHGSGGRWVESF
                     LGGGALEVETIRAERDTLFGGVAPEPIDRNLAPLRERVSERRALVGLATDGDADRVGA
                     VGDGGATMTMHEVVPLILLHLARSRALAGGVVRTFSQSVLLKRIAAAHNLPLHETPIG
                     FKYVADLMLREDILIGAEESGGIGLRGHIPERDGILNSLLFLEAIIASNKTPTALVRE
                     MHREFGEFYFGRRDLHIEVARGQSLVGELAVNPPHNVGGDAVRSVETLDGTKLVFEDE
                     SWLLFRQSGTEPVLRIYAEATSTAKMQTLLDEGQQIVLRTED"
     gene            2063..2896
                     /gene="mutM"
                     /locus_tag="AC159P_11855"
     CDS             2063..2896
                     /gene="mutM"
                     /locus_tag="AC159P_11855"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF002211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HIR3696185.1"
                     /translation="MPELPEVELVARILDRLLRGRSVVAARLLRPGLAPDTSPRSFAL
                     RLRGASFESAARRGKHVLLHLGGARVLVTHLRMTGRFLLLPAERELPPHTHAVFLLDD
                     GRRLVFTDQRHFGFMKLVRADELDEAKELRALAPEPFAEDFTPDYLRRTLSRTRRVLK
                     EVLLDQTKVTGLGNIYAAEVLFLARVNPFLAAAELSPRRVPRLHRAIQDVLAESIAHG
                     STMNVDPENIDGSYYGGAYEGRWRVYDREGEPCLACGARVRRVSRGGRSTYFCPRCQK
                     S"
     gene            2996..>3259
                     /locus_tag="AC159P_11860"
     CDS             2996..>3259
                     /locus_tag="AC159P_11860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696186.1"
                     /translation="MKHPFKAAAVIVSLVALLCLARVAHAQRPHAPKSQALVTNARVA
                     ADGAMSGPLHVTVGGKEKKVADGAVAAWVIQGGRTVVYSGRDGA"
BASE COUNT          502 a         1219 c         1040 g          498 t
ORIGIN      
        1 aactactcgt tcgagtacgg cctcttccca gacctcctgc gccgcgaaga atccttccac
       61 gcctacgtcc cccgcgacgc ctactggatc gacatcggca cgcccgcgcg ctacctccaa
      121 gtccaccaag acctgctcgc ggggcgcgtc cgcggcgtac aggttgaccg ccccgccgtg
      181 gacgcgtacc gcgagggcgg cgcggagatc gacgacctct cgctcgtcgg cgcggactgc
      241 gtcgtcaagc ccggcgcgca gatcgtcaac tccgtcctcg gcgagggctg ccacgtcgaa
      301 gagaaggcga gcgtcgtcaa ctcggtcgtc tggccgcaca cgcgcatcgc cgcgggcgcg
      361 cacgtcgcgg gcgccgtcat cgggcgcggc tgccacgtcg ggcgcgcgac gcacatcgac
      421 ggcgccgtcg tcggcgacaa gtcttcgctc accgactaca cgcagctcgg gggcggcctg
      481 tgatgacggg gcgcgagcgt ccggcccgca tcaggttcgg caccgacggc tggcgcgcgc
      541 tcatcgccga cgagttcacc ttcgacaacc tcgaacgcgt cgcccaggcc tacgccgact
      601 tcctcctcag cgagttggcc atgcacacga cggttgacga gtcccaacac gtcgcttcac
      661 atctcgcgga gacgctgcgc gtcgtcgtcg gctacgaccg ccgcttcctc tccgagcagt
      721 tcgcgcggcg ggcggcggaa gtcctgagcg gcaacggcct cgctgtctcg ctcttcgaac
      781 gggacgtgcc gacgccgctc gtctcgtggg ccgtgcgcga aggcggcgcg gcgggcggcg
      841 tcgtcatcac cgcctcgcac aaccctgcgc ccttcaacgg cttcaagatc aaggccccgt
      901 ggggcgggag cgcgaccgag cagacgacgc tcgccgtcga agcgctcgtc gatgcgcacg
      961 cgcagcgccg cgcgacggaa cttcccgcga gcgttcacga agggttgggc gaggcggcgg
     1021 agagctaccg gcggcaggtc gcctcgttcg tcgatctcga acgcatccgg gccgcgcgcg
     1081 cgcgcgtcgt cgtcgatccg atgcacggct cgggcggtcg ctgggtcgag agcttcctcg
     1141 gcggcggcgc gctcgaagtc gagaccatcc gcgccgaacg cgacacgctc ttcggcggcg
     1201 tcgcgcccga gccgatcgac cgcaacctcg cgccgctcag agagcgcgtc tcggagaggc
     1261 gcgcgctcgt cggcctcgcg accgacggcg acgccgaccg cgtcggcgcc gtgggcgacg
     1321 gcggcgcgac gatgaccatg cacgaggtcg tgccgctcat cctccttcac ctcgcgcgct
     1381 cgcgcgcgct cgcgggcggc gtcgtccgca ccttctcgca gtccgtcctg ctcaagcgca
     1441 tcgccgcggc ccacaacctg ccgctccacg agacgcccat cggcttcaag tacgtcgcag
     1501 acctcatgct ccgcgaagac attctcatcg gcgccgaaga gtccggcggc atcggcctgc
     1561 gcggccacat ccccgagcgc gacggcatcc tgaactccct gctcttcctt gaagcgatca
     1621 tcgcctcgaa taagacgccg accgcgctcg tgcgcgagat gcaccgcgag ttcggcgagt
     1681 tctacttcgg tcgccgcgac ctccacatcg aagtcgcgcg cggccaatcg ctcgtgggcg
     1741 agcttgccgt caacccgccg cacaacgtcg gcggcgacgc ggttcgatcg gtcgaaaccc
     1801 tcgacggcac caagctcgtc ttcgaagacg aatcgtggct cctcttccgc cagtccggca
     1861 ccgagcctgt cctccgcatc tacgccgaag cgacctcgac cgccaagatg caaacgctgc
     1921 tcgacgaagg acaacaaata gtactgagga ctgaggactg agtaataatt gatgagccat
     1981 ggacgatgaa tggatgatga gaagcacgct gtctctcatc attcaacctg tcctctcact
     2041 cagtcctcag tccttgagtt ttatgcccga actacccgaa gtcgaactcg tcgcgcgcat
     2101 cctcgaccgc ctgttgcgcg ggcggagcgt ggtggcggcg cggctgctgc ggcccggtct
     2161 tgcgccggac acgtcgccgc gcagcttcgc gctccgcctg cgcggcgctt cgttcgagtc
     2221 cgccgcgcgg cgcggcaagc acgtgctgct ccacctcggc ggcgcgcgcg tcctcgtcac
     2281 gcacctgcgg atgaccggca ggttcctgct gctccccgcc gagcgcgagc tgccgccgca
     2341 cacgcacgcc gtcttcctgc tcgacgacgg acgccgcctc gtcttcaccg accagcgcca
     2401 cttcggcttc atgaagcttg tccgcgccga cgagctggac gaggccaaag agctgcgcgc
     2461 gctcgcgccc gagccgttcg cggaggactt cacgcccgac tacctgcgcc gcacgctctc
     2521 gcgcacgcgc cgcgtgctca aagaagtcct gctcgaccag acgaaggtga cgggcctcgg
     2581 caacatctac gcggcggaag tcctcttcct cgcgcgcgtc aacccgttcc tcgccgccgc
     2641 ggagttgtcg ccgcgccgcg tcccgcgcct gcaccgcgcc atccaggacg tgctcgccga
     2701 atccatcgcc cacggctcga ccatgaacgt cgatcccgag aatatcgacg gcagctacta
     2761 cggcggcgcc tacgaggggc gctggcgcgt ctacgaccgc gagggcgaac cctgcctcgc
     2821 gtgcggcgcg cgcgtccgcc gcgtctcgcg cggcggtcgc tcgacctact tctgcccgcg
     2881 ctgccagaaa agttaaggcc gacgacgacc gccgccggtg atatactacg gcccacttag
     2941 tcatcttaat caggacatct taagcagcac atccttcgaa gggggtcgct ctcgcatgaa
     3001 gcatccattc aaagccgccg ccgtcatcgt gtctctggtc gcgctcctgt gcctcgcgcg
     3061 cgtcgcgcac gcgcagaggc cgcacgcgcc caagtcgcag gcgctcgtca ccaacgcgcg
     3121 cgtcgcggcg gacggcgcga tgtccggccc gctccacgtc accgtcggcg gcaaggagaa
     3181 gaaggtcgcg gacggggcgg tcgcggcgtg ggtcatacag ggcgggcgaa cggtcgtcta
     3241 ctccgggcga gacggggcg
//
LOCUS       DBNGOH010000611         1784 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1008696, whole genome shotgun sequence.
ACCESSION   DBNGOH010000611 DBNGOH010000000
VERSION     DBNGOH010000611.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1784)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1784)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1784
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1008696"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="AC159P_11865"
     CDS             complement(<1..183)
                     /locus_tag="AC159P_11865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015755401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HIR3696187.1"
                     /translation="MATYQLTVNGQRREVTVDPSTPLLWVLREDLKMTGTKYGCGVSA
                     CGACTVHVAGVATRSCV"
     gene            complement(195..830)
                     /locus_tag="AC159P_11870"
     CDS             complement(195..830)
                     /locus_tag="AC159P_11870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696188.1"
                     /translation="MPRVRAASLLFSAIFFACGSWMIVSGTLGQSKGAKPSAAGRAQG
                     LAAWEQVYSVLTHPRCINCHTATNYPQQGDDRRRHVANVVRGGPEGKGVPGLNCAGCH
                     QESNADSTGVPGGHNWHLAPLSMKWQDLNDQPLSSAEVCRAITDRSKNHDMDGPKLLK
                     HHEEEALVLWAWNPGRRPDGTARTLPPLSHAEFVAATRRWVEAGTPCPSGK"
     gene            1203..>1784
                     /locus_tag="AC159P_11875"
     CDS             1203..>1784
                     /locus_tag="AC159P_11875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696189.1"
                     /translation="MNDKREQTEIEQAAPVFEGFVLKALDKSSKAKARQAVSRVLGQG
                     WVVRPFGDDATDFEVTRRTQKGARLSARRAWEKTYALRTQPGVTYAEPMFTVPVSGPP
                     GFRAEAVEQIGEEVAPPRPSSSAAAPFAARGLFTGDGDLPESNDPAWSIKAVKVPEAW
                     KRFPPGQEPGEKIVVGHPDTGYTQHPEIASRLIT"
BASE COUNT          307 a          610 c          590 g          277 t
ORIGIN      
        1 cacgcacgag cgcgtggcga cgccggcgac gtgcaccgtg caggcgccgc acgcgctgac
       61 gccgcagccg tacttggtgc ccgtcatctt caagtcttcg cgcagcaccc agagcagggg
      121 ggtcgaaggg tcgaccgtca cctccctgcg ctgcccgttc acggtgagct gatacgttgc
      181 catcgtcgat gctcctattt tcccgaaggg caaggcgtcc cggcctcgac ccagcggcgg
      241 gtcgcagcga cgaactccgc gtgtgaaagc ggcggcagtg tgcgcgccgt gccgtcgggg
      301 cggcggcccg gattccaagc ccacagcacg agcgcttctt cttcatgatg cttgagcaat
      361 ttcgggccgt ccatgtcgtg gttcttcgaa cggtcggtga tggcccgaca cacttcggcg
      421 ctcgacaaag gctggtcgtt caggtcttgc cacttcatcg agaggggcgc cagatgccag
      481 ttgtgcccgc cgggcacgcc cgtgctgtcg gcgttggact cctgatggca gcccgcgcag
      541 ttcagaccgg gcacgccttt gccctcgggg ccgccgcgca ccacgttggc gacgtggcgg
      601 cggcggtcgt cgccctgctg cgggtagttc gtggcggtgt gacagttgat gcagcgcggg
      661 tgggtcagga cggaatagac ctgctcccac gcggcgaggc cctgcgcgcg accggcggcg
      721 gaaggtttcg cacctttcga ctggccgagc gtgcccgaga cgatcatcca tgacccgcac
      781 gcgaagaaga tggccgagaa gagaaggctc gcggcgcgaa cacgcggcat gatgttcctc
      841 ctgctgaaca cgtcgggccg tgtacagccg tggcagaccg gtgtgcgtac tgtaagaaca
      901 cgcgcatatt aactccaaag agggcgcggt ggtgagttga atttttcgcc ccgtgacaac
      961 cgcttcattc accgcgcgtc tcggtaatca tgcgtgcgga cgacgtggct tcgaaatccg
     1021 aaatctctcg tccgccatcg caccggcggc ctccgggctg cctcggaatc gtgttgacat
     1081 cgaaccgcgg cgggcatatc gttcccactc cacgaggcgc tttcacgtca acgagcggca
     1141 agagccgtaa cggcctcacc tcgaacacaa cgcggcacgc gccgcccgcc gaggcgaaca
     1201 ccatgaacga caaacgcgag cagacggaga tcgagcaggc cgcccccgtc ttcgagggtt
     1261 tcgtcctcaa ggcgctcgac aaaagcagta aggcgaaggc gcggcaggcc gtcagccgcg
     1321 tcctcgggca gggctgggtg gtgcgcccct tcggcgacga cgcgaccgac ttcgaagtca
     1381 cgcgccgcac gcagaagggc gcgcggctct cggcccgccg cgcgtgggag aagacctacg
     1441 ccctgcgcac gcagcccggc gtgacctacg ccgagccgat gttcacggtg cccgtctccg
     1501 gcccgcccgg cttccgcgca gaagccgtcg agcagatcgg agaagaagtc gcgccgccgc
     1561 gcccgtcgtc aagcgccgcc gcgcccttcg cggccagagg tcttttcacc ggcgacggcg
     1621 acctgcccga gagcaacgac cccgcgtgga gcatcaaggc tgtgaaggtg cccgaggcct
     1681 ggaaacgctt ccctcccggg caggagccgg gcgagaaaat cgtcgtcggc caccccgaca
     1741 caggctacac gcagcacccg gagatcgcct cgcgcctgat taca
//
LOCUS       DBNGOH010000612         5705 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_823288, whole genome shotgun sequence.
ACCESSION   DBNGOH010000612 DBNGOH010000000
VERSION     DBNGOH010000612.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5705)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5705)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5705
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_823288"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..637)
                     /locus_tag="AC159P_11880"
     CDS             complement(<1..637)
                     /locus_tag="AC159P_11880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D-related
                     protein"
                     /protein_id="HIR3696190.1"
                     /translation="MSVTLPPQLEVLDQPLDTQMTISFGPQHPSTHGVLRLELVLDGE
                     MIVKCVPDIGYLHTGMEKLFEYKKYQQGIVITDRMDYLNPLGNNLGYCMAVEKLLGLE
                     IPERAQTIRVLMCELQRIGSHMVWLGTHALDIGAMTVFFYCFREREKILNLIEAASGE
                     RMTPSYFRIGGLMMDLPAGFERRVRQFADKFIKGVDEFNTLLTGNTIFQGRT"
     gene            complement(725..1267)
                     /locus_tag="AC159P_11885"
     CDS             complement(725..1267)
                     /locus_tag="AC159P_11885"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012549.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HIR3696191.1"
                     /translation="MDIEREKQIEAASKQPRATYRAPSVETLRALGEEHEGWFAETTE
                     ALGEVTIVVPRERIAEVCAHMKAAPGLEFNMLADLCGVDRGPEEEPRFEVNYHLFSTT
                     RHHRLRLKVLLNEDDPHVPTVTSVWRTANWHERETFDMFGVVFDGHPDLRRILLPDEW
                     QGHALRKDFPLRGYEPYSLT"
     gene            complement(1396..2034)
                     /locus_tag="AC159P_11890"
     CDS             complement(1396..2034)
                     /locus_tag="AC159P_11890"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015746298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045271 - respiratory chain complex I
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HIR3696192.1"
                     /translation="MGLENVIAEALPEILTTRLDSLVNWARKSSLWPATFGLACCAIE
                     MMATTDARHDLARFGSEVFRASPRQADVMIVSGRVSRKMAPVLRRIYDQMPEPKWVIS
                     MGACATSGGVFDNYAIVQGVDKVVPVDVYIPRCPPRPEMLIHAVMMLQEKIKKESLLD
                     RKDVSAEDAYEAVPPGTLPATGVSATNEAAAQEPPPPRPYTIASRHERRRSS"
     gene            complement(2174..2509)
                     /locus_tag="AC159P_11895"
                     /pseudo
     CDS             complement(2174..2509)
                     /locus_tag="AC159P_11895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015722616.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
     gene            2801..3775
                     /locus_tag="AC159P_11900"
     CDS             2801..3775
                     /locus_tag="AC159P_11900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696193.1"
                     /translation="MITGFNTDIQHGGVTYHVQTEDKGLETPLILSLVYVGGAIIASK
                     RSRYEDLIESGFDEQALTERLQRQHKLICAAIRSGRVEDLKRMTGREEPAKAEKPAKK
                     EKAPKRAAAPREEQPEAATEKQHPARTVKQREEPREPEAPHEPATAPILFGEDQYLDE
                     IYRREAAEAAAATAVETNVEAEPVADVAPPPSPAPPPPVHVPAEAVRVLEGAQGQPFA
                     EATGAEEIYLTLLEDEGEFRAGQLATIKIRVGRGQFGQHAVADAAVTIKVLGTTFRPL
                     ILSTKTGVDGVAVVRALLPRFTSGRAAIIIRASSGEEFAEMRRIIHQA"
     gene            3790..4005
                     /gene="thiS"
                     /locus_tag="AC159P_11905"
     CDS             3790..4005
                     /gene="thiS"
                     /locus_tag="AC159P_11905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010489495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HIR3696194.1"
                     /translation="MRINVNGEEHAAQEGTTVEALVAQLALRPERLAIELNGEVVRRA
                     DWPRTTLGEGDRVEIVHFVGGGRRSRQ"
     gene            4267..5058
                     /locus_tag="AC159P_11910"
     CDS             4267..5058
                     /locus_tag="AC159P_11910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005007332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HIR3696195.1"
                     /translation="MSTSIETSTESFKIGDRSFASRLIVGTGKYRSHDEMKEAHRESG
                     AEMVTVAVRRVPLDRSSESFLDHLHPELTILPNTAGCYTAEEAVRTARLAREALQTDL
                     VKLEVIGDQVTLFPDNEQTLEAARVLVREGFTVLPYFTDDLIMAKKLLDAGCAAVMPL
                     AAPIGSGLGVQNPANLRIMREQLPDALIIVDAGVGTASDAAVAMELGADAVLMNTAIA
                     EAQHPGQMARAMRLAIEAGRLAYLAGRMPKRLYASASSPLAGVVR"
     gene            complement(5112..5705)
                     /locus_tag="AC159P_11915"
                     /pseudo
     CDS             complement(5112..5705)
                     /locus_tag="AC159P_11915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008575533.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005882 - intermediate filament [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
BASE COUNT         1045 a         1834 c         1881 g          945 t
ORIGIN      
        1 tcgtgcgccc ctgaaaaatc gtgttgccgg tgagcagcgt gttgaactcg tcaacgccct
       61 tgatgaactt gtcggcgaac tgccgcacgc gccgctcgaa gcccgcgggc aagtccatca
      121 tcagcccgcc gatgcggaaa taggagggcg tcatgcgctc gcccgaggcg gcctcgatga
      181 ggttgagaat cttctcgcgc tcgcggaagc agtagaagaa gacggtcatc gcgccgatgt
      241 cgagcgcgtg cgtgccgagc cagaccatgt gcgagccgat gcgctgcaac tcgcacatca
      301 ggacgcggat cgtctgcgcg cgctcgggga tttcgaggcc gagcaatttt tcgaccgcca
      361 tgcagtagcc gaggttgttg ccgagcgggt tgagatagtc catccggtcg gtgatgacga
      421 tgccctgctg gtacttcttg tactcgaaga gcttctccat ccccgtgtgg aggtagccga
      481 tgtcgggcac gcacttgacg atcatctcgc cgtcgagcac gagttcgagc cggagcacgc
      541 cgtgcgtcga ggggtgctgc ggcccgaacg agatggtcat ctgcgtgtcg agcggctggt
      601 cgagaacctc aagctgtggc ggaagagtga cgctcatatc aattgcggat ttgggattgt
      661 ggatttcggg ttctcgggaa tgcggctgtc tttcaatccg caatccgaaa accgcattcc
      721 gcaatcaggt taggctgtac ggctcgtagc cgcgcagcgg gaagtctttg cggagcgcgt
      781 ggccctgcca ttcgtcgggc aggaggatgc ggcgcaggtc cgggtggccg tcgaagacga
      841 cgccgaacat gtcgaaggtt tcgcgctcgt gccagttggc ggtgcgccag accgaggtca
      901 cagtggggac gtgcgggtcg tcttcgttca ggaggacttt caggcgcagg cggtggtgcc
      961 gggtcgtcga gaagagatgg tagttgacct cgaagcgtgg ctcctcctcg gggccgcggt
     1021 caacgccgca gagatccgcg agcatgttga attcgaggcc gggcgccgcc ttcatgtgcg
     1081 cgcaaacttc cgcgatccgc tcgcgcggga cgacaatcgt cacctcgccc agcgcctccg
     1141 tggtctccgc gaaccagccc tcgtgctcct cgccgagcgc gcgcaacgtc tcgacggaag
     1201 gcgcacggta ggtcgcgcgc ggctgcttcg acgcggcctc gatctgcttc tctctctcga
     1261 tgtccataag tagcttttgg cgattggctg ttagcgatta gcttcgagcg ccggacagca
     1321 ttcacctttc attcaaacgt ccaagggcgg ggaaggggaa agctaacagc ttatcgctaa
     1381 cagctaacag cttcttcacg acgagcggcg gcgctcgtgg cgcgaagcga tggtgtaggg
     1441 cctcggcggc ggaggctcct gcgccgcggc ctcgttggtc gcggagacgc cggtcgcggg
     1501 gagcgtgccg ggcgggacgg cctcgtaggc gtcctcggcg gacacgtctt tgcggtcgag
     1561 cagcgattcc ttcttgatct tctcctgaag catcatcacg gcgtggatga gcatctccgg
     1621 gcgcggcggg cagcgcggga tatacacatc cacggggacg accttatcga cgccctgcac
     1681 gatggcatag ttgtcgaaga cgccgcccga cgtggcgcac gcgcccatcg agatgaccca
     1741 cttcggctcg ggcatctggt cgtagatgcg ccgcagcacg ggcgccatct tgcgcgaaac
     1801 gcggcccgag acgatcatca cgtcggcctg ccgcgggctg gcgcggaaga cctcggagcc
     1861 gaagcgcgcg aggtcgtggc gcgcgtccgt ggtggccatc atttcgatgg cgcagcaggc
     1921 gaggccgaag gtggccggcc agaggctcga cttgcgcgcc cagttcacca gcgagtcgag
     1981 ccgcgtggtc agaatttcgg gcaaagcctc ggcgattaca ttctctaggc ccatcgtaaa
     2041 acctctctct cgcggctcag gcggcgcgcg gcgcggcggt tcccggcgcc ctcttcgcgg
     2101 cgagccgccg tctctcgtcc tgcgcgtgca gcgcctgcac ctcggcctct gcctcgacgc
     2161 gtgcgcgcgc gccccactcg aacagcccct tcttccagac gtagacgtag ccgaccagca
     2221 gcagcacgac gaacagcatc atctcgacgt agatcagagc gccgagacct tccgcgatga
     2281 acttcctgaa gacgaccgcc caggggatga ggaagatgag ttcgatgtcg aagaggatga
     2341 agaccatcgc gatcaggtag aacttgaccg agaagcgttc gtgcgccgag ccgacagggt
     2401 ctttgccgca ctcgtagggc atcgtcttcg tgcgcgtcgc cttgtgctgc ccgacgaact
     2461 gggagaggat gatgttgccg acggcgaaac ccgcggcgac cccgaacatc agcaaaatcg
     2521 ggaagtaatc ggccaggttg aacacaccaa ccctccctca gcttgcgctg acgaaatggc
     2581 tctcaggcca gccattcggt agattgagaa tcatggcaca aaccaatcct agtccatgag
     2641 gtggagggtg tcaagcgcct ttcccggcgg gcgaaatgcg tttttttgaa ggagattgac
     2701 cggcttccga gtgctatgct actttccgga atggccctcg ccaaaggagc cgttccccct
     2761 gtcgcaagcc cgcacgttcg aaaactagtc agcttgacca gtgatcacag gattcaacac
     2821 agacatccag cacgggggcg tcacctacca cgtccagacg gaggacaagg ggctggagac
     2881 gccgctcatc ctctcgctcg tctacgtcgg cggcgcgatc atcgccagca agcgctcgcg
     2941 ctacgaagac ctcatcgagt ccggcttcga cgagcaggcg ctcaccgagc gactgcaacg
     3001 ccagcacaag ctgatctgcg ccgccatcag gtcgggccgc gtcgaagacc tcaagcggat
     3061 gaccgggcgc gaagagccgg cgaaggccga gaagcctgcg aagaaagaga aggcgccgaa
     3121 gcgcgccgcc gcgccgcgcg aagagcagcc cgaagcggcg accgaaaagc agcacccagc
     3181 ccggaccgta aaacagcgcg aagaaccgcg ggagccggag gcgccgcacg agccggcgac
     3241 cgcgccgatt ctgtttggcg aggatcagta cctcgacgaa atctaccggc gcgaagccgc
     3301 ggaagcggcg gcggcgacag cggtcgagac gaacgtcgag gccgagccgg tcgcggacgt
     3361 ggcgccgccg ccgagtccgg cgcctccgcc ccccgtgcac gtgccggctg aagccgtccg
     3421 cgtgctcgaa ggggcgcagg gccagccgtt cgcggaggcg acgggggccg aggagattta
     3481 cctgacgcta ctcgaagacg aaggcgagtt ccgcgcgggg cagttggcga cgatcaagat
     3541 acgcgtcggg cgcgggcagt tcgggcagca cgccgtcgcg gacgcggcgg tcacgatcaa
     3601 ggtactcggc accacgttcc gcccgctcat cctctcgacc aagacgggcg tggacggcgt
     3661 cgcggtcgtg cgcgcgctgc tgccgcgctt cacctcgggc cgcgccgcca tcatcatccg
     3721 cgccagctcc ggcgaggagt tcgcagagat gcgccgcatc atccaccagg cttaggggac
     3781 acgaaggttt tgcgcatcaa cgtcaatggg gaagagcacg cggcgcagga aggaacgacc
     3841 gtcgaggccc tggtcgcaca actcgcgctc aggcccgagc gcctcgccat cgaactcaac
     3901 ggcgaggtcg tccgccgcgc cgactggccg cgcacgacgc tcggcgaagg cgaccgcgtc
     3961 gagatcgtcc acttcgtcgg cggaggccga cggagtaggc agtaggcagt aggcagtagg
     4021 cagtcaagac agaagaagag cgaagagctt gagaggtaaa gtgcaacgcc tcttcgttca
     4081 tcaagcggtc agagagaggc tttgcacttt acctctcaag tctcttcgtg cttttgtttt
     4141 tactgcctac tgcctgctgc ttactgccta ctccgttttt ctttcgcctc gttcgagtgt
     4201 aaattatcca gtagacgttt cagggattcg gcacgcgggc atcaacaacc gtttatccga
     4261 ccaactatgt caacaagtat cgagacgagc actgaatcat tcaagattgg cgacaggagt
     4321 ttcgcgtcgc ggctgatcgt gggcacgggc aagtaccgca gccacgacga gatgaaggag
     4381 gcgcaccgcg agtcgggcgc ggagatggtg acggtcgcgg tgcgccgcgt cccgctcgac
     4441 cgcagctccg aatcgttcct cgaccacctc catccggagc tgaccatcct gccgaacacg
     4501 gcgggctgct acacggctga ggaggcggtg cgcacggcgc ggctcgcgcg cgaggcgctc
     4561 cagacagacc tcgtcaagct cgaagtcatc ggcgatcagg tgacgctttt ccccgacaac
     4621 gagcagacgc tcgaagccgc gcgcgtcctc gtcagggaag gcttcaccgt gctgccttac
     4681 ttcacggacg atctcatcat ggcgaagaag ctgctcgacg cgggctgcgc cgccgtgatg
     4741 ccgctggccg cgcccatcgg ctccggcctc ggcgtgcaga acccggcgaa cctgcgcatc
     4801 atgcgcgagc agttgccgga cgcgctgatt atcgtggacg cgggcgtcgg caccgcgagc
     4861 gacgcggcgg tggcgatgga gctgggcgcg gacgcggtgc tcatgaacac ggccatcgcg
     4921 gaggcgcagc accccggcca gatggcgcgg gcgatgcgcc tcgccatcga ggcgggccgc
     4981 ctcgcatacc tcgctggccg catgccgaaa cgcctctacg ccagcgcctc aagcccgctc
     5041 gcgggcgtcg tgcgttaggg ccgaatgaac cggctcttcg gcggccccct catcaagtaa
     5101 tcgaagaaga cgcgcgcggg gtcgtttctc ctgaactcga aaaggggaga cagcgtggcg
     5161 cccgtcgcgt tgccgcccgc gcaggtttgc gtaggcgagc tgcgattgcg cggagcgggc
     5221 gggccggtcg agaagtcggc ggagttgtcc cccgaatcga cgcagccgtc caacttcctg
     5281 agcacggcgg tcgtgttgga cggcgcgggc gccgccgcac cctccgcaca gttcgcgctc
     5341 ccgtatccga caaaatccac caacgccgca cccgacggac acgcgccgct cagcgcggtc
     5401 gtgctgttga cgagcgccac cttgcccgcg gtcgcggcca acgcgatgct gcccgtcgcg
     5461 tcgggcgtcg gcagtgaagt cgtcccgccc gtccccgccg cctcttgaat caggtagaac
     5521 cggcccggcg cgagagagcc tgaaagtgcc gtgacctgcc aactcgtgcc ggtcgccccc
     5581 gcgtactgca cagaccaccc gcttaaatca accgtcgtat cgccgcggtt gaacagctcg
     5641 ataaagtcgt tgctgaacgt ggcgccgctg ttcccgccgc cgccgtagac ctgactgatg
     5701 accac
//
LOCUS       DBNGOH010000613         2329 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_640382, whole genome shotgun sequence.
ACCESSION   DBNGOH010000613 DBNGOH010000000
VERSION     DBNGOH010000613.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2329)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2329)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2329
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_640382"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(89..>2329)
                     /locus_tag="AC159P_11920"
     CDS             complement(89..>2329)
                     /locus_tag="AC159P_11920"
                     /inference="COORDINATES: protein motif:HMM:NF049774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF7619 domain-containing protein"
                     /protein_id="HIR3696196.1"
                     /translation="ISPPVAAIGFQHTWVECPGRATFDGNAGSESFQPTNISGPRSYF
                     VGATDIGGISHVRLESGCGPPERWDDMLFVPGLAPPPPGEAELSLVKRAPQSIRPSTD
                     VVYDFDVQNSGPDRGRNVQVMDFLPPELTFAAAFPAATPADPPGRVYTMNFGDVDAAT
                     TASGLMRLAAPPFASAYNSPGLTCASKPVNVALVTATTRGDSPAGNLAVAVSSFDPAA
                     RAGFGEICGNLIDDNCDGLTDCDDSVCGCYPPLIAPPNPPSCIFLPPFSTCTSVSPAS
                     SPSARERRAQFESCGPFDDGHGGTALLPAFCCDGEPNSGFQEQQCRVPIDPNRKEAEP
                     GVNAAGFGYTEAGGLINYTLRYENIGDADAHDVAVIDVLPTDLDAATLVMSEGGVYDP
                     SNRAIIWRDAVVPPDVPRSVSFSARVRSDAPHGTRVRNTASVIFPDAVPPSRIDTNPV
                     EHVVIGPGILVEPVPKVFQCTETAPGEWRVDLVNEGYGFAYNVTASILNAPASVSVSE
                     ATATFAHPSDPDPARLSTVIPLAATASTDTVRFDTETPGDPCGALTWRITYRNSRGES
                     FTRDVQDAPDRDRDAVADTADNCPDAYNPTQADADGDGRGDVCANVAPDCGGAYPSVR
                     DASTPTFRKLAVTILGVTDPDGDPVTVTINRVMQDEPTGNGADGDACPDAYIPPNSAT
                     AYVRDERSGNGDGRVYTIYFTATDSRGASCQGSVKVAIPYDPGGGAVDDGPVYDSKVC
                     SGKANR"
BASE COUNT          322 a          715 c          924 g          368 t
ORIGIN      
        1 ggagttcttc tccgtgccct tcgcttccga cggcccctac cgtcaacgag gtgagagatg
       61 cgaggggtgt gcgtcggcaa agtccgggtc agcggttcgc ctttcccgaa cagaccttcg
      121 agtcgtacac ggggccgtcg tccacggctc cgccgcccgg atcgtatggg atggcgactt
      181 tgacgctccc ctgacagctc gcgccgcggc tgtcggtcgc cgtgaagtag atcgtgtaga
      241 cgcgaccgtc gccgttgccg ctgcgctcgt cgcgcacgta agcggtcgcg ctgttgggcg
      301 ggatgtaggc gtctgggcag gcatcgccgt cggcgccgtt gccggtcggc tcgtcctgca
      361 tgacgcggtt gatagtgacg gtgacggggt cgccgtccgg gtctgtcacg ccgaggatcg
      421 tgacggcgag cttacggaag gtgggcgtcg aggcgtcgcg caccgaaggg taggcgccgc
      481 cgcagtcggg cgcgacgttg gcgcacacgt cgccccgacc gtcgccgtcc gcgtcagcct
      541 gcgtcgggtt gtaggcgtcg gggcagttgt ccgcggtgtc cgccacggcg tcgcggtcgc
      601 ggtcgggcgc gtcctgcacg tcgcgcgtga aagattcgcc gcgcgagttc cggtaggtga
      661 tgcgccaggt gagagcgccg caggggtcgc cgggcgtctc tgtgtcgaag cggacggtgt
      721 cggtgctcgc ggtggcggcg agcgggatga cggtcgagag tcgcgccggg tcagggtcgg
      781 acgggtgcgc gaaggtggcc gtggcctcgg agacgctgac cgaagccggg gcgttgagga
      841 tggaggccgt cacgttgtag gcgaagccgt agccctcgtt gacgagatcg actcgccact
      901 cgccgggcgc ggtctctgtg cactgaaaga ccttcgggac aggctcgacg aggatgccgg
      961 ggccgatgac gacgtgctcg accgggttgg tgtcgatgcg cgagggaggg acggcgtcgg
     1021 ggaagatgac ggacgccgtg ttgcggacgc gcgtcccgtg cggggcatcg cttcgcacgc
     1081 gcgccgagaa gctgacgctg cggggcacgt cgggcggcac gaccgcgtcg cgccagatga
     1141 tggcgcggtt cgacgggtcg taaacacctc cttcactcat gacaagcgtc gcggcgtcga
     1201 ggtctgtcgg cagcacgtcg atgacggcca cgtcgtgcgc gtccgcgtcg ccgatgttct
     1261 cgtagcgcag cgtgtagttg atgaggccgc ccgcctccgt gtagccgaag ccggcggcgt
     1321 tcacgcccgg ctcagcctcc ttgcggttcg ggtcaatcgg cacgcgacat tgctgctcct
     1381 gaaagccgct gttcggctcg ccgtcgcaac agaaggcggg cagcaaagcg gtgccgccgt
     1441 gcccgtcgtc gaaggggccg cagctctcga actgcgcgcg gcgttcgcgc gcggacgggg
     1501 acgaggcggg cgacacggag gtgcaggtgc tgaagggcgg caggaagatg cacgagggcg
     1561 ggttgggcgg cgcgatgagc ggcgggtagc agccgcacac ggaatcgtcg cagtccgtca
     1621 ggccgtcgca gttgtcgtcg atcaggttgc cgcagatttc gccgaagccc gcgcgcgcgg
     1681 cggggtcgaa ggacgagacc gcgaccgcga ggttgcccgc gggcgagtcg ccgcgcgtcg
     1741 tggcggtgac gagcgcgacg ttgacgggct tcgacgcgca ggtgagtccg gggctgttgt
     1801 acgccgaggc gaagggcggg gcggcgagac gcatgaggcc gctcgcggtg gtcgcggcgt
     1861 ccacgtcgcc gaagttcatc gtgtagacgc ggcccggggg gtcggcgggc gtggcagccg
     1921 gaaacgcggc ggcgaaggtc agctcgggcg ggagaaaatc catgacctgc acgttgcgtc
     1981 cgcggtcggg gccgctgttc tgcacgtcga agtcgtagac gacatcggtg ctggggcgaa
     2041 tcgactgggg cgccctcttg acgagcgaca gctccgcctc gccgggcggg ggcggggcca
     2101 agccgggcac gaagagcatg tcgtcccagc gctccggcgg cccgcacccg ctttcgagac
     2161 ggacgtgcga gatgccgccg atgtcggttg cgccgacgaa gtagctccgc ggcccgctga
     2221 tgttcgtggg ctggaagctc tcgctcccgg cgttgccgtc gaaggtcgcg cgaccggggc
     2281 actcgaccca agtgtgctgg aagccgatgg cggcgaccgg cgggctgat
//
LOCUS       DBNGOH010000614         6613 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3941244, whole genome shotgun sequence.
ACCESSION   DBNGOH010000614 DBNGOH010000000
VERSION     DBNGOH010000614.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6613)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6613)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6613
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3941244"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(66..482)
                     /locus_tag="AC159P_11925"
     CDS             complement(66..482)
                     /locus_tag="AC159P_11925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012457012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HIR3696197.1"
                     /translation="MSQDDASPEWRRRVSTGSPYEPLIGISRAVRAGRFVAVAGTAPL
                     GPDGRTVAPGDAAAQARRCFEIVGQALEAAGASLADVVRTRILLTRIEDWQQVARVHG
                     EFFRAVRPANTVMQVSRFIDPEWLVEVEADAVVEEP"
     gene            complement(578..1003)
                     /locus_tag="AC159P_11930"
     CDS             complement(578..1003)
                     /locus_tag="AC159P_11930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017775628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0006972 - hyperosmotic response [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0033194 - response to hydroperoxide
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HIR3696198.1"
                     /translation="MRRKGSAVWRGGLRDGKGTVSTESGVLADAQYSFGTRFEEGKGT
                     NPEELIGAAHASCFSMALSKQLEDAGAKAEEISTTATVRLEKTDAGFTVTSVHLDVTV
                     KAPGLDESGFENAANNAKAGCPISRLLNAEITMDAKLAS"
     gene            complement(1153..2688)
                     /locus_tag="AC159P_11935"
     CDS             complement(1153..2688)
                     /locus_tag="AC159P_11935"
                     /inference="COORDINATES: protein motif:HMM:NF012234.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR3696199.1"
                     /translation="MTNQLCPAQQDALRVLMHGMSVGSLSVLFGGTGLGKTTVLAELH
                     HAVGGAFLNMKDFVDAMRGQHPLAVEETFERLVMDALLAHDHVIVDDLHMLNNVVCCG
                     HFYPRTGLLNAPAKTLADYAAGAGKKLVFGTEGTPPLPLYQRAYGSGIEDFGAADYAA
                     VCRAYLDPEAAARLDYRKIHRFAAKLNAHQLKSACLWLRGDEALDTERFIEYLRSQRL
                     ASNVDLGEVADVDLSDLKGVDEVLRSLEANIILPLEDDELAAELSLKPKRGVLLAGPP
                     GTGKTTIGRALARRLRSKFFLIDGTFISGTGNFYGQVHHIFEAAKQNAPSIIFIDDSD
                     AIFESGEELGLYRYLLTVLDGLESASAGRVCVMLTAMDVGHLPPALIRSGRIELWLEM
                     SLPGAEARADILAAHLGQLPPSLAAADTAPLVAATEGFTGADLKRLVEDGKTLYAYDR
                     AQRREPRPATEYFLSAVETVRANKERYAEAEARARRQRPARPVWYGMAGDAAFGTNFF
                     VQE"
     gene            complement(2776..3678)
                     /locus_tag="AC159P_11940"
     CDS             complement(2776..3678)
                     /locus_tag="AC159P_11940"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012673.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA];
                     GO_process: GO:0050427 - 3'-phosphoadenosine
                     5'-phosphosulfate metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ family
                     protein"
                     /protein_id="HIR3696200.1"
                     /translation="MKALGEETTEETTKGAYEHELRVVLELARAAGAAALGYYGGPLR
                     VEHKEEFDEPVTQADRAVNELIVRALGESFPADGILAEESVDTKARLGRERVWMVDPL
                     DGTKGFIAGTGDFAVQIGLAEAGESVLGVVYAPATDVMYWAARGRGAWVERPVETEAS
                     SSRVERLRVSGNTDLQSMRLAESRSHRGPKMDTVVRALGIREEIKSHSVGIKVGLIVE
                     RQCDVYIHLSGKTKQWDTCAPEAILREAGGLMTDLWGEPLRYNTPDVLNRHGLVSTNG
                     VVHTTVVGRIRPLLAGFGRARVEV"
     gene            complement(3675..5228)
                     /locus_tag="AC159P_11945"
     CDS             complement(3675..5228)
                     /locus_tag="AC159P_11945"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012503.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent decarboxylase
                     family protein"
                     /protein_id="HIR3696201.1"
                     /translation="MPDMTDERRASSLDIADEEARELAARAVGLALECLAPGGELPVF
                     PDTSAEEVARLFRLPLPEEGAGLDALERDFREVVNHVRLNNHPRMFGYIASPATHVGA
                     FASLVASSLNSNVTSWRSAPAATEVERTVVGWLAEMIGYAGGESRCGGLLTSGGSMAN
                     LVALYVAHRTKAREGSDALRAESAHDASQEGLWNAGAPMTLYASDQVHLSIPKAADVL
                     GLGRKQVRAVPSDENFMLDVRALRELIDADARRGLRPFCVVASAGTVSTGAVDPLADI
                     ARVARERALWFHVDGAYGVLAAADESRRHLFAGLEAADSVSLDPHKWLYTPVDCGCLL
                     LRRAELARSAFAGTEEGYIKVFESGADEAFAFWDYGVELSRPFRALKVWATLRYYGAR
                     KIAAAIAEDNALAEYLAARVRESEDFELLAPATLGICCFRYVPPAVRRELEQVVGEDR
                     ERFEARLDALNERVMLRAQRAGRAYVSNAVLRGRFALRASITNFRTTREDLDITLDAL
                     RAAASDESL"
     gene            5440..6570
                     /locus_tag="AC159P_11950"
     CDS             5440..6570
                     /locus_tag="AC159P_11950"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR3696202.1"
                     /translation="MSLPGFATTEGTARYRDRFAGEVAEQHFRFEQQLWLSSVGLGTY
                     LGGWDEETDSAYAEAVVRAVELGANVVDTAANYRFQRSERSIGAALKALTSGGGFARE
                     EIVVCTKGGYLPFDGQPPAGQAGVRAYVEETFVRTGVATFEEIAAGSHCMTPRYVEHQ
                     VAQSLRNMDLETLDVYYVHNPETQLSVVTRAEFETRLRAAFEQLERERAAGRIRHYGV
                     ATWNGFRVEPNAKGYHALERMWELAVEAGGDEHGFRFVQLPFNLAMPEALALGNQPYR
                     GARLSLLEAAKALGVTVVASASIMQGRVARDLPEHIREPLGSLATDAQTAIQFVRSTP
                     GVTTALVGMSSAAHVEENLGLARVAPARAEDYSRLFEQEGGE"
BASE COUNT         1000 a         2319 c         2271 g         1023 t
ORIGIN      
        1 gaagcgtagt ctcggacgtt caagtttcgc atcgaccacg aatccgcaat ccgaattccg
       61 caatcctacg gttcttcgac gaccgcgtcg gcctcgacct cgacgagcca ctcggggtcg
      121 atgaagcgcg agacctgcat gacggtgttc gcggggcgga cggcgcggaa gaactcgccg
      181 tggacgcgcg ccacctgctg ccagtcctcg atgcgcgtga gcaggatgcg cgtccgcacc
      241 acgtccgcga ggctcgcgcc cgcggcttcc agcgcctgcc cgacgatctc gaagcagcgc
      301 cgcgcctgcg ccgccgcgtc gcccggcgcg accgtgcgcc cgtcggggcc gagcggcgcg
      361 gtgcccgcca cggcgacgaa ccgccccgcg cgcaccgcgc gcgagatgcc gatcaaaggc
      421 tcgtacggcg acccggtcga gacgcgccgc cgccactcag ggcttgcgtc atcctgcgac
      481 atgaatctcc ctgcatgaat atgaagtctg aagtctgaag tctgaagtcc gagcaaaaag
      541 cagtcggttc agacttctga cttctgactt ctgactttca agaagcgagc ttcgcgtcca
      601 tcgtgatctc ggcgttgagc aggcgcgaga tggggcagcc ggccttggcg ttgttggcgg
      661 cgttctcgaa gccggactcg tcgaggccgg gcgccttgac cgtcacgtcg aggtggacgg
      721 aggtgacggt gaagcccgcg tcggtcttct ccagcctgac ggtggccgtc gtgctgatct
      781 cctcggcttt cgcccccgcg tcttcgagct gcttcgagag cgccatcgag aagcagctcg
      841 cgtgggccgc gccgatcagc tcctccgggt tcgtgccctt gccctcttcg aagcgcgtgc
      901 cgaacgaata ctgcgcgtcc gcgagcacgc cgctctcggt cgagaccgtg cccttgccgt
      961 ccctcagacc gccccgccac accgcagaac ctttacgtct catctcttcc tcctgaaatt
     1021 gggttgtaaa ctgccgccac tcttaactcc tctggccgaa gcctcgcgcg caaagtcacc
     1081 ccgccctggg ggcggcgcgc gcgtccggcc ccggccccgg cgcgcgccgg agggaacgtc
     1141 aacgcgcggc gctcactcct gcacgaagaa gtttgtgccg aaggccgcgt cgcccgccat
     1201 gccgtaccag acgggccgcg cggggcgctg gcggcgggcg cgggcctccg cctccgcgta
     1261 gcgctccttg ttggcgcgca ccgtctcgac cgcggagagg aagtattcgg tcgcggggcg
     1321 cggctcgcgc cgctgcgcgc ggtcgtaggc gtagagggtc ttgccgtcct cgaccagccg
     1381 cttgaggtcg gcgccggtga acccttcggt cgcggccacg agcggcgccg tgtcggcggc
     1441 ggcgagtgag ggcgggagct ggccgaggtg cgcggcgagg atgtcggcgc gcgcctcggc
     1501 gccgggcagg ctcatctcca gccacagctc gatgcgcccc gagcggatca gcgcgggcgg
     1561 caggtggccc acgtccatcg ccgtcagcat cacgcacacg cgcccggcgc tcgcgctctc
     1621 cagcccgtcg aggacggtca gcaggtagcg atagaggccc agctcctcgc cgctctcgaa
     1681 gatggcgtcg ctgtcgtcga tgaagatgat cgagggggcg ttctgcttgg cggcctcgaa
     1741 gatgtgatgc acctgtccgt agaagttccc ggtgcccgag atgaacgtgc cgtcgatgag
     1801 gaagaacttg ctgcggagcc ggcgggcgag ggcgcgcccg atggtcgtct tgcccgtgcc
     1861 gggcggcccg gccagcagca cgccgcgctt cggcttgagg ctcagctccg cggccagctc
     1921 gtcgtcctcc agcggcagga tgatgttggc ctccaggctg cggaggacct cgtccacgcc
     1981 cttcaggtcg gagaggtcaa cgtccgcgac ctcgccgaga tcgacgttgc tcgccaggcg
     2041 ctgcgagcgc aggtactcga tgaagcgctc ggtgtcgagc gcctcgtcgc ccctcagcca
     2101 caggcacgcg ctcttgagct ggtgcgcgtt gagcttcgcg gcgaagcggt gaatcttccg
     2161 gtagtcgagg cgggccgccg cctccgggtc gaggtaggcg cggcagacgg ccgcgtagtc
     2221 cgcggccccg aagtcttcga tgccggagcc gtaggcgcgt tggtagagcg gcagcggcgg
     2281 cgtgccctcg gtgccgaaga cgagtttctt gccggccccg gcggcgtagt cggcgagcgt
     2341 ctttgccggc gcgttcagca ggcccgtgcg cgggtagaag tggccgcagc agacgacgtt
     2401 gttgagcatg tgcaggtcgt ccacgatgac gtggtcgtgc gcgaggagcg cgtccatcac
     2461 cagccgctcg aaagtctcct cgacggcgag cgggtgctgc ccgcgcatcg cgtccacgaa
     2521 gtccttcatg ttgaggaagg cgccgcccac cgcgtggtgg agttcggcca gcacggtcgt
     2581 cttgccgagg cccgtccccc cgaagagcac ggacaggctg ccgacagaca tgccgtgcat
     2641 caacacccgg agggcgtctt gctgtgcggg gcagagttga ttcgtcatcg gtgtggaaat
     2701 ctctttcggt tgatctcgct cgactcgccc cgcgcctcat cccgccggcg gcggcggcga
     2761 accggggccg cgattttaca cctcgacgcg cgcgcgcccg aaccccgcga gcagcgggcg
     2821 aatgcgcccg acgaccgtcg tgtgaacgac gccgttggtg gagacgaggc cgtggcggtt
     2881 gagcacgtcc ggcgtgttgt agcgcagcgg ctcgccccag aggtcggtca tcaggccgcc
     2941 ggcctcgcgc aggatggcct cgggcgcgca cgtgtcccac tgcttcgtct tccccgagag
     3001 gtggatgtac acgtcgcact ggcgctcgac gatgaggccg accttgatgc cgaccgagtg
     3061 cgacttgatc tcctcgcgga tgccgagcgc gcgcaccacc gtgtccatct tcgggccgcg
     3121 gtgcgaacgg ctctccgcca gtctcatgct ctgcaaatcc gtgttgcccg agacgcgcaa
     3181 acgctcgacc cgcgaactcg acgcttcggt ctcgactggc cgctcgaccc acgcgccccg
     3241 cccgcgcgcg gcccagtaca tcacgtcggt cgcgggcgcg tagacgacgc cgagcacgct
     3301 ctcgcccgct tcggccagcc cgatctgcac ggcgaagtcg cccgtgcccg cgatgaagcc
     3361 cttcgtcccg tccagcggat cgaccatcca cacgcgctcg cgcccgagcc gcgccttcgt
     3421 gtccacggac tcttcggcga ggatgccgtc cgcggggaac gactcgccga gggcgcgcac
     3481 gatcaactcg ttcaccgcgc ggtcggcctg cgtcacaggc tcgtcgaact cctccttgtg
     3541 ctcgacgcgc aaaggaccgc cgtagtaccc gagcgccgcc gcgcccgccg cgcgcgccaa
     3601 ttccaacacg acgcgcagct cgtgctcgta cgcgcccttc gtcgtctctt ccgtcgtctc
     3661 ttcccccaaa gctttcataa agactcgtcg ctcgccgccg cgcgtagcgc gtcgagcgtg
     3721 atgtccaggt cctcccgcgt cgtgcggaag ttcgtgatcg aggcgcgcag cgcgaagcgc
     3781 ccgcgcaaga cggcgttcga gacgtaggcg cggcccgcgc gctgagcgcg cagcatgacg
     3841 cgctcgttca gcgcgtcgag ccgcgcctca aaccgctcgc ggtcttcgcc cacgacctgc
     3901 tccagctcgc ggcgcacggc gggcggcacg tagcggaagc agcagatgcc gagcgtcgcg
     3961 ggcgcgagca gctcgaagtc ttcgctctcg cggacgcgcg cggcgaggta ctctgcgagc
     4021 gcgttgtctt cggcaatcgc ggcggcgatc ttgcgcgcgc cgtagtagcg cagcgtcgcc
     4081 cacaccttca gcgcgcggaa cgggcgcgac agctcgacgc cgtaatccca gaaggcgaaa
     4141 gcctcgtccg cgccgctctc gaagactttg atgtagccct cttcggtgcc cgcgaacgcg
     4201 gagcgcgcca gctccgcgcg gcgcagcagc aggcagccgc aatcgaccgg cgtgtagagc
     4261 cacttgtgcg ggtcgagcga gaccgagtcg gcggcttcga gtcccgcgaa gagatgacgg
     4321 cgcgactcgt ccgccgcggc gagcacgccg taggcgccgt ccacgtggaa ccagagcgcg
     4381 cgctcgcgcg cgacgcgcgc gatgtcggcg agcggatcga ccgcgcccgt cgagacggtg
     4441 cccgcgctcg ccacgacgca gaagggccgg aggccccgcc gcgcgtccgc gtcgatcagc
     4501 tcgcggagcg cgcgcacgtc caacatgaaa ttttcatccg aaggaactgc gcgcacctgc
     4561 ttgcgcccga ggccgagcac gtccgccgcc ttcggtatcg agaggtgaac ctgatccgac
     4621 gcgtacagag tcatcggcgc gcccgcgttc cacaagcctt cttgcgaggc gtcgtgggcg
     4681 gactccgcgc gaagcgcgtc ggagccttcg cgcgccttcg tgcggtgcgc gacgtagagc
     4741 gcgacgaggt tcgccatcga gccgccgctc gtcagcagcc cgccgcagcg gctttcccct
     4801 ccggcgtagc cgatcatctc tgcaagccag ccgacgaccg tgcgctcgac ctcggtcgcc
     4861 gcgggcgccg agcgccacga agtgacgttc gagttgagcg acgaggcgac gagcgaggcg
     4921 aacgctccga cgtgcgtggc gggcgaagcg atgtagccga acatgcgcgg gtgattgttg
     4981 aggcggacgt gattgacgac ctcgcggaag tcgcgttcga gcgcgtcgag gccggctccc
     5041 tcttcgggga gcggcaggcg gaaaagacgc gcgacctctt cggcggacgt gtccgggaag
     5101 acgggaagct cgccgcccgg cgcgagacat tcgagcgcca acccgaccgc gcgcgcggcg
     5161 agttcgcgcg cctcttcgtc ggcgatgtcg agcgacgatg cgcgcctctc gtccgtcatg
     5221 tcgggcatga cggcaaccct accacagcgc cgcccctttc taaaggactg aggactgagg
     5281 actgaggact gagtatgaag acgagtcttt cactcagtcc tcggtcctca gtcctcggtc
     5341 ctttggtaaa atgcgctgac atcgcggcgc gcggcctgct acacttcgcg cgcaccgatt
     5401 tttccgcttc cgacagacac cgaaggagac gagcacgaga tgtcgctgcc aggattcgcc
     5461 acgaccgagg ggacggcgcg ctaccgcgac cgcttcgccg gggaggtcgc cgagcaacac
     5521 ttccgcttcg agcagcagct ctggctctcg tcggtcggcc tcggcacgta cctcggcggg
     5581 tgggacgagg agaccgacag cgcttacgcg gaagccgtcg tgcgcgccgt cgagctgggc
     5641 gcgaacgtcg tggacacggc ggcgaactac cgcttccagc ggagcgagcg ctcgatcggc
     5701 gcggcgctca aggctttgac ctcgggcggc ggcttcgcgc gcgaggagat cgtcgtctgc
     5761 acgaagggcg gctatcttcc tttcgacggc cagccccccg cggggcaggc gggcgtgcgc
     5821 gcctacgtcg aggagacgtt cgtgcggacg ggcgtcgcca cgttcgaaga gatcgcggcg
     5881 gggagccact gcatgacgcc gcgctatgtg gaacatcagg tcgcgcagtc gctccgcaac
     5941 atggatttgg agacgctgga cgtgtactac gtccacaacc ccgagacgca gctctcggtc
     6001 gtcacgcgcg ccgagttcga gacgcggctg cgcgcggcct tcgaacagct cgaacgcgag
     6061 cgcgccgcgg ggcgcatcag gcactacggc gtggcgacgt ggaacggctt ccgcgtcgag
     6121 ccgaacgcga agggctacca cgcgctcgaa cgcatgtggg aactcgccgt cgaggcgggc
     6181 ggcgacgaac acggcttccg cttcgtgcag ctccctttca atctggcgat gcccgaagcg
     6241 ctcgccctcg gcaaccagcc gtaccgcggc gcgcgcctct cgctgctcga agcggcgaag
     6301 gcgttgggcg tcacggtcgt tgccagcgcc tcgatcatgc aggggcgcgt ggcgcgcgac
     6361 ctgcccgaac acatccgcga gccgctcggc tcgctcgcca cggacgcgca gaccgccatc
     6421 cagttcgtcc gctcgacgcc cggcgtcacc accgcgctcg tcggcatgag cagcgccgcc
     6481 cacgtcgaag agaacctcgg gctggcgcgc gtcgcgcccg cgcgcgccga agactacagc
     6541 cgcctcttcg agcaggaggg cggcgaatag gagtcgaaaa caggatgagt gacgggaagg
     6601 ctctgttggt ggt
//
LOCUS       DBNGOH010000615         2066 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2106507, whole genome shotgun sequence.
ACCESSION   DBNGOH010000615 DBNGOH010000000
VERSION     DBNGOH010000615.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2066)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2106507"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            514..>2066
                     /locus_tag="AC159P_11955"
     CDS             514..>2066
                     /locus_tag="AC159P_11955"
                     /inference="COORDINATES: protein motif:HMM:NF052065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF7948 domain-containing protein"
                     /protein_id="HIR3696203.1"
                     /translation="MKSRQPVVTGPAVAALSVLFVIACLVYGAAGLGLARAETTSTGA
                     AAAARRTAATMPARRPADFVENRGQWDAPSKFVARKGATTAAFERTAIRLRFGKGQAT
                     ALGLVFEGASPAAALAGEGRREGRYNFFVGDSSRWRANVAAYGGVLYRGLYEGIDVRV
                     RETDERFEYDLLLAAGADLGRVVIRVDGASRVEVAEDGSLLLHTGGGTLRQTPPRTWE
                     ELPDGGRRPLECRFRKIDARRYGFAAAGRDARLPLVIDPGLEWSTYLGGGEWDEILDL
                     AAAPDGSGDVIAVGATASDDFTGRAALWTGFVARFDANGGLVYKTVLGGSDRERIYGL
                     AVNAEGEPVVVGESYSLDFPTTPGAFDTTHGIAADGRASADAFVARLNASGNQLLFST
                     YLGTSDIEWAYAVALTPTGAVVVAGETTSDNFPTTVGAFDRTRSCCTPLGQGSFSIRD
                     AFVARLKLDGNGSADLKYSTFIGGSDTDEGYAVALDPTNPNDVVLGGVTYSTVSTARF
                     QTTAGTLRP"
BASE COUNT          290 a          755 c          703 g          318 t
ORIGIN      
        1 ggggtcaacc tctgggccgc gaacttcggg agcgactcgc tctcgaaagg cgccgccacg
       61 caatgacgcc gccccgcttg acgaagcctt agcgcttcga acccgcgcga agcgccgccg
      121 cttcgcgcgc cacacggggg cggccctcgg tcggaccgcc ccctttcttg aacgcgacgg
      181 ttgacgacgg tgtcagcatc cgcggaaacg tttaaccccc gagttctatt cgagagcgct
      241 ttgcgacgtg gccgagggcc gccgtcccgc gcgggcgggc ttcggccacg tcgcgccgtg
      301 aaatttcttc gccgcgaatg tatcagcggc gccttccccc ggctctgttc tttcagacct
      361 caagtactac gaacaacgtc acgtgtcggc gcggcccctg ccctgttgac gtgaaatgga
      421 atagcgcggc gcactcgccg ccggcggcag tccacctttg tcaacggtcg tccctcatcc
      481 cgaccgggta acgagcctgt aggaggtgcc agcatgaaga gtcgtcagcc ggtcgtcacc
      541 ggccccgccg tcgccgccct ttcggttctc ttcgtcatcg catgtctggt ttacggggcc
      601 gccggtctcg gcctcgcgcg ggccgagacg acttcgaccg gcgcggctgc cgcggcgcgg
      661 cgcacggcgg cgacgatgcc ggcccgccgg cccgccgact tcgtcgagaa ccgcgggcag
      721 tgggacgcgc cctcgaagtt cgtcgcgcgc aagggcgcga ccaccgcggc cttcgagcgg
      781 acggccatca ggctccgctt cgggaagggg caggcgaccg cgctcggcct cgtcttcgag
      841 ggcgcctcgc ccgccgccgc gctcgcgggc gaaggacggc gcgaagggcg ctataacttt
      901 ttcgtcggcg attcttcgag gtggcgcgcg aacgtcgcgg cctacggcgg cgtgctctac
      961 cgcgggctgt acgaaggcat cgacgtgcgc gtgcgcgaga cggacgagcg cttcgaatac
     1021 gacctgctgc tcgccgccgg cgcagacctc ggacgggtcg tcatccgcgt tgacggcgct
     1081 tcgcgtgtgg aggtcgcgga ggacggctcg ctgctgctgc acaccggcgg cggcacgctg
     1141 cggcagacgc cgccgcgcac gtgggaagag ttgccggacg gcgggcggcg cccccttgag
     1201 tgccgcttcc ggaagatcga cgcgcggcgc tacggcttcg cggcggcggg gcgcgacgcg
     1261 cggctgccgc tcgtcatcga ccccggcctc gaatggtcta cgtacctcgg cggcggcgag
     1321 tgggacgaga tactcgacct cgccgcggcg ccggacggct cgggcgacgt gatcgcggtc
     1381 ggcgcgacgg cctccgacga cttcacgggg cgcgccgcgc tctggacggg cttcgtcgcg
     1441 cgcttcgatg cgaacggcgg cctcgtctac aagaccgtcc tcggcggctc cgaccgcgag
     1501 cgtatttacg gcctcgccgt caacgccgag ggcgaacccg tagtcgtcgg cgagagctat
     1561 tcgctcgact tcccgacgac gcccggcgcc ttcgacacga cgcacggcat cgcggcggac
     1621 gggcgcgcga gcgccgacgc cttcgtcgcg cgcctcaacg cttcgggcaa ccagttgctc
     1681 ttctcgacct acctcggcac gagcgacatc gagtgggcct acgccgtcgc gctcacgccc
     1741 acgggcgccg tcgtcgtcgc gggcgagacg acctcggaca acttcccgac gaccgtcggc
     1801 gccttcgacc gcacgcgcag ttgctgcacg cccctcggcc agggcagctt ctccatccgc
     1861 gacgccttcg tcgcgcggct caagctcgat ggcaacggct ctgccgacct caagtactcg
     1921 accttcatcg gcggcagcga cacggacgaa ggctacgcgg tcgcgctcga cccgacgaac
     1981 ccgaacgatg tcgtgttggg cggcgtcacc tactcgaccg tcagcacggc gaggttccaa
     2041 accaccgcgg gcactttgcg tccaag
//
LOCUS       DBNGOH010000616         2396 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1375154, whole genome shotgun sequence.
ACCESSION   DBNGOH010000616 DBNGOH010000000
VERSION     DBNGOH010000616.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2396)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2396)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2396
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1375154"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            133..993
                     /gene="cysT"
                     /locus_tag="AC159P_11960"
     CDS             133..993
                     /gene="cysT"
                     /locus_tag="AC159P_11960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012187818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902358 - sulfate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HIR3696204.1"
                     /translation="MRARAADLGAAVAVARRRPWGRWGLRAVAVSYLALMILLPVSAI
                     LRSGLQDGLGAFWADVTNPTATAALRLTLFAAVVTTAINVVMGTMTAYVLVRYEFPGR
                     GLLNALVDIPFAIPTLVTGVMLVVLYGPQRTLGAWLEARGVEVIFATPGIILALLLVT
                     YPFLIRAVQPVLSELDKAQEEAAYTMGASKWTTFRTIILPAITPAIITGSLLNFARAL
                     GEFGSIIVVAGNIPGKTLVSAVYVFGQVESYNQRGASAVSLVLLALSFSLMLVVERMQ
                     KRWGVHHVAE"
     gene            980..1843
                     /locus_tag="AC159P_11965"
     CDS             980..1843
                     /locus_tag="AC159P_11965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902358 - sulfate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit"
                     /protein_id="HIR3696205.1"
                     /translation="MSPNEGEVGFQPHVGAGGESRRPGAVEWVLIAAVALYAAVLLLG
                     PFAAVIWGAFSEGFGAFVEGATSPEALSALKLTLLLAVGATVINTIFGVCIAWVLVRD
                     PFRGRGFLNALVDLPFVVSPVIAGLMLILLFGRDGWLTPLADAAGVKVVFALPAMLLV
                     TTFVSLPFVVREVMPVLAEVGTEQENAAYTMGATGWQAFRHITLPAIRWGVLYGVSLT
                     FARSVGEFGAVLVVSGGVSGLTETSTLYIFRSLDDRNYVAAYATAAVLAALSFLILFA
                     MEFFRRRGEEG"
     gene            complement(2027..>2396)
                     /locus_tag="AC159P_11970"
     CDS             complement(2027..>2396)
                     /locus_tag="AC159P_11970"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3696206.1"
                     /translation="EGQYLEAQRQLEEALKLDKSNSWVYYNLGLIYLEQRNYDPAIDN
                     FKAALSGSLRPTWVEAWAHIKMGNAYDAKGDRPRAVFEYKQAVQSGIEFDNAQKVAQG
                     FIQTPYDPKAASQQASTGAN"
BASE COUNT          328 a          744 c          846 g          478 t
ORIGIN      
        1 tacacgaaga tgttcgacga gctgcgcggg cagaagtaga agctagaggc tagaggctag
       61 aggttggaga aaagtcattt cgtttttact agcctttagc ctcaagcctc tagtctctga
      121 gtggagcgac agatgagggc acgggcggcg gacttggggg cggcggtcgc ggtcgcgcgg
      181 aggcgaccgt gggggcgctg gggcctgcgg gcggtggcgg tgagctacct cgcgctgatg
      241 attttgctgc cggtctcggc gattttgcgg agcggtttgc aggacgggct gggggctttc
      301 tgggcggacg tgacgaaccc gacggccacc gccgcgctcc ggctgacgct gttcgccgcc
      361 gtcgtcacga ccgccatcaa cgtcgtgatg gggacgatga cggcctacgt gctcgtgcgt
      421 tacgagttcc cggggcgcgg cctgctgaac gcgctcgtgg acatcccttt cgccatcccg
      481 acgctggtga cgggcgtcat gctcgtcgtg ctctacgggc cgcagcgcac cttaggcgcg
      541 tggctcgaag cgcgcggcgt cgaggtcatc ttcgccacgc cgggcatcat cctcgcgctg
      601 ctgctggtga cttatccttt tctgatccgc gcggtgcagc ccgtactgtc ggagctggac
      661 aaggcgcagg aggaggcggc ctacacgatg ggcgcgtcga agtggacgac gttccgcacg
      721 atcatcctgc cggcgatcac gccggcgatc atcacgggat cgctgctgaa cttcgcgcgc
      781 gcgctcggcg agttcgggtc gatcatcgtc gtcgcgggca acatccccgg caagacgctg
      841 gtctcggcgg tctacgtctt cgggcaggtc gaatcctaca accagcgcgg ggcgagcgcc
      901 gtctcgctgg tgctgctggc gctctcgttt tcgctgatgc tcgtggtcga acggatgcag
      961 aagcgttggg gtgtacacca tgtcgccgaa tgaaggggag gtcgggttcc agccgcacgt
     1021 cggcgcgggc ggcgagtcgc ggcgaccggg cgcggtcgag tgggtgctga tcgcggcggt
     1081 cgcactgtac gcggcggtgc tgctcctggg gccgttcgcg gcggtcatct ggggcgcgtt
     1141 ctccgagggc ttcggtgcct tcgtcgaggg cgccacctcg ccggaggcgc tctcggcgct
     1201 caagctcacg ctcctgctcg cggtgggcgc gaccgtcatc aacacaatct tcggcgtctg
     1261 catcgcgtgg gtgctggtgc gcgacccgtt ccgcgggcgc ggctttttga acgcgctcgt
     1321 cgatctgccg ttcgtcgtct cgccggtcat cgcggggctg atgctgatcc tgctgttcgg
     1381 gcgcgacggg tggctgacgc cgctggcgga cgccgccggg gtgaaggtcg tcttcgcgct
     1441 cccggcgatg ctgctggtga cgacgttcgt ctcgctcccc ttcgtcgtcc gggaggtgat
     1501 gccggtcttg gcggaggtcg gcaccgagca ggagaacgcg gcctacacga tgggcgcgac
     1561 cggctggcag gcgttccgcc acatcacgct gcccgccatc cgctggggcg tgctctacgg
     1621 cgtgtcgctc accttcgcgc gctcggtcgg cgagttcggc gcggtgctcg tcgtctcggg
     1681 cggcgtgagc ggcctcactg agacctcgac gctctacatc ttccgctcgc tcgacgaccg
     1741 caactacgtc gccgcctacg cgaccgccgc cgtgctcgcc gcgctctcct tcctcatcct
     1801 cttcgcgatg gagttcttcc gcagacgcgg cgaggaaggg taagacgaca tagggcgaaa
     1861 aggggaaaag gggaagaggc gaaaagggaa gacgagaaac agcctgcctc tcatcaacca
     1921 tcgatgagag gcaggctgtt tctcgtcact caactgagga taagaagcag cgtgcttgtt
     1981 atcctccctt ttcgcctctt ccccttttcc ccttttcgcc cgcggttcag ttggcgccgg
     2041 tgctggcctg ctgcgaggcg gccttcgggt cgtagggcgt ctggatgaag ccttgcgcga
     2101 ccttctgcgc gttgtcgaac tcgatgccgg actggacggc ctgcttgtat tcgaaaacgg
     2161 cgcgggggcg gtcgcccttg gcgtcgtagg cgttgcccat cttgatgtgc gcccacgcct
     2221 cgacccaggt ggggcgcagg ctgccggaga gcgccgcctt gaagttgtcg atggccgggt
     2281 cgtagttgcg ctgttcgagg tagatgagac cgaggttgta gtagacccac gagttcgact
     2341 tgtcgagctt gagcgcctcc tccaactgcc gctgcgcttc gaggtactgc ccctcg
//
LOCUS       DBNGOH010000617         8909 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1009095, whole genome shotgun sequence.
ACCESSION   DBNGOH010000617 DBNGOH010000000
VERSION     DBNGOH010000617.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8909)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8909)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8909
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1009095"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            82..2028
                     /locus_tag="AC159P_11975"
     CDS             82..2028
                     /locus_tag="AC159P_11975"
                     /inference="COORDINATES: protein motif:HMM:NF045042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan lyase family protein"
                     /protein_id="HIR3696207.1"
                     /translation="MRNTVLLVLTLCVVAAAARAQETTPTPTPSSIPPNFSHYTLNPN
                     PKPTALGWATARIEEKLGRGVSALRTEAGGVYVGWRLLKQDPPDIAFNVYRSTAGGAA
                     VRLNAAPITKTTDFLDAKAPPDREHAWWVRAVVSGTEQEPSVRATLAAGSPARQYRSF
                     KIRDDIPVTSIDKIGVGDLDGDGEYDFVVKRPGGEIDPSFLTRHSPDTFKIEAYKSDG
                     TFLWRRDLGWSIQLGIWFSPFHVADLDGDGKAEIAIKTGEGDPRDKNGQVLKGPEYLS
                     IWDGMTGKDIDRTDWIPRGQLSDWGDYSGNRSNRHMLGVAYLDGKTPSVVIFRGTYGL
                     MRAEAFYLKDKKLHKVWSWTNERAGWRYQGQGQHAIHFADLDGDGADEVLNGSIAIES
                     DGRMMWSTGMGHGDRFYVTDIDPARPGLEIWYSYEDPHPRNGVSLWDAKTGDIIFGTD
                     EETADNQVGSALVGDIDPAYPGMEVWGEKFFYTAKGERIPGPVPPQQGLVWWDADPLR
                     ELHTFGRGAGRGPAINPATGAPATGQDTTQGTAQGGGQGARPAGQGGPNFMRGSTVSK
                     WKGSVLTSNIQGRTVLWGDIAGDWREEIVTFAQGEVRIYGTVIPATDRRVTLMQDPLY
                     RSEVGLVVQGYTHVPVTSYYLGVK"
     gene            complement(2530..3075)
                     /locus_tag="AC159P_11980"
     CDS             complement(2530..3075)
                     /locus_tag="AC159P_11980"
                     /inference="COORDINATES: protein motif:HMM:NF012311.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HIR3696208.1"
                     /translation="MSLGMAAVDSFRNDPACKAVRRMADAGVVVVVAAGNNGKDEAGQ
                     KVYGQVHSPGNEPSAITVGASNTYGTDTGSDDTVATYSSRGPTRSYWTDVSGTRYYDH
                     LIKPDLVAPGNKLISAQATGNKLITEYPQMDPGVNVGFSTNSRLMYMSGTSVSAPVVA
                     GTAALMLRMQPKLTPNLVKMI"
     gene            complement(3221..3985)
                     /locus_tag="AC159P_11985"
     CDS             complement(3221..3985)
                     /locus_tag="AC159P_11985"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3696209.1"
                     /translation="MRKASDAFGQQLLALHAGRRQGAEIIERADGYVGAGQDPARYFS
                     EYKDWSRAERRAIRLAAGRVLDIGCGAGRHSLYLQSRGLDVTGIDLSPGAVRVCRMRG
                     LKKALVRSIDEVDRFAPGSFDTVLMLGNNFGLFGSRAGARRTLRKLARVTTDGARIIA
                     QSLDPHATDDPEHLRYHRRNLSRGRLAGQLTMRVRFGRAVGPWFDYLLVSRREMEEVL
                     EGTGWLVERFLDADGPTYVAVIRKQAPDEEHPARRR"
     gene            4069..4617
                     /locus_tag="AC159P_11990"
     CDS             4069..4617
                     /locus_tag="AC159P_11990"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013122.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HIR3696210.1"
                     /translation="MLYNVRQSYTPALEAHEFNPHALIIDTETVGTGSSIEVVEVALC
                     DFEGRVLLDTLVRPVYNPPPRSTPAQRFDSTEFDAAPDWTDVWPALAALAENKLLVAY
                     NAAFDRRALAAMCARNRLPSEAGRGWRCAMQLAKRVLGAKKNLLLSEACARFGLEAGS
                     HRAAADVLATHRLLKRLVTPEL"
     gene            complement(4763..6223)
                     /gene="gatA"
                     /locus_tag="AC159P_11995"
     CDS             complement(4763..6223)
                     /gene="gatA"
                     /locus_tag="AC159P_11995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011551579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HIR3696211.1"
                     /translation="MTAPEKTIEHISSALSGGETTARAVAAAAVGAAEKLNDALNAFL
                     QIDRAGAIRRAEELDKSAPDSPLRGIPIAIKDNICVRGLQASCGSRILGDYQPPYDAT
                     VVERLKATGAVVVGKTNCDEFAMGSSNENSAFGPVRNPWDTERVPGGSSGGSAAAVAA
                     RIVPVALGSETGGSVRQPAALCGIVGAKPTYGRVSRYGLVAFGSSLDQVSVFGLTVPD
                     AAHVLGVIAGRDERDATTADVPVPAYASGLTGDIKGARVGVPRALFGEGLDAEVRAGV
                     EAAIEAYRELGTEIVDVELPHAKYSIAVYYIIATAEASSNLARYDGVRYGYRAEEAPE
                     LRQMYRRTRDEGFGAEVKRRIMLGTYVLSSGYYDAYYLQAQQVRTLIKQDFARAFEKC
                     DAILTPTSPTPAFRLGEKTDDPLAMYLSDIYTCMANLAGVPGISVPCALSSEGLPIGF
                     QLMGAHWSEATLFRLASAYERAHPLGARPRVVADAN"
     gene            complement(6330..6653)
                     /gene="gatC"
                     /locus_tag="AC159P_12000"
     CDS             complement(6330..6653)
                     /gene="gatC"
                     /locus_tag="AC159P_12000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HIR3696212.1"
                     /translation="MPITEADIRKIADLAHLEITDEELRHLGPQVAAIVAYVEQLNEL
                     DTSQVEPAIGGLTPEGGRTEAVREDRTRPSLGQELALAEAPDPSHGHFRVPKVIAGSE
                     EKEDF"
     gene            complement(6729..7196)
                     /locus_tag="AC159P_12005"
     CDS             complement(6729..7196)
                     /locus_tag="AC159P_12005"
                     /inference="COORDINATES: protein motif:HMM:NF017103.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HIR3696213.1"
                     /translation="MDELIQSLPKLLRAAGEAEEVLEAAAFVAWRRAAGEGVRAAAVP
                     FRLYRKTLVVAVPDQTWQKQLEKVSGQLLFRVNSLLGQALVTFIEFRIDARTVQAERD
                     RLRLQTGTPTDEDHALRRSGELRAAASAIRDETLRRRFLIAAGSYLNAQERKP"
     gene            7298..7945
                     /locus_tag="AC159P_12010"
     CDS             7298..7945
                     /locus_tag="AC159P_12010"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014263275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009146 - purine nucleoside triphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HIR3696214.1"
                     /translation="MEKERSLQAGGAQGAWLGALVPLVLASGSPRRAEILRAVGWPFE
                     ASAPEVDESRREGERAEDYVRRLAREKAEAGASTRLFGLVLGADTVVVVERELLGKPR
                     DEADARRMLRALSGRWHEVLTGVALVRAESGRALVGVERTRVRFAQMSDEEIDWHVST
                     GEPLDKAGAYAVQGRAALFIEAIEGDYWNVVGLPVRLVYELVRKLEEEKEEGRKG"
     gene            complement(8079..8633)
                     /locus_tag="AC159P_12015"
     CDS             complement(8079..8633)
                     /locus_tag="AC159P_12015"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF020123.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin family protein"
                     /protein_id="HIR3696215.1"
                     /translation="MLTPKRRSLLALTAALLFSLSSLALALPTGAAAQVDFRLKTADG
                     GEISSGMLRGDVVVLAFGASWLPLSKAQMQGVQELSDEFAQRNVRVYWVSTDSDKPQS
                     KNYATDEQLRTLARKNGLKVAVLRDPDGTFFKKTGADQIPSVIILDREGRVAGAPVGG
                     ISTDPKNKLVNVLSGRLNTLLGGQ"
BASE COUNT         1494 a         2900 c         3049 g         1466 t
ORIGIN      
        1 ttttccctac acaggcgacg acctccgttt tgacgagaac cgacaagccc tgtcgaaagg
       61 cgtggtatgc ttgattcaat gatgagaaac acggtgcttc tagtcctgac gctctgcgtc
      121 gtcgccgccg cggcccgcgc gcaggaaacg acgccgaccc cgacgccttc gtccatcccg
      181 ccgaacttca gccactacac gctcaacccg aacccgaagc cgaccgccct cggctgggcc
      241 acggcgcgca tcgaggagaa gctgggacgc ggcgtctccg ccctgcgcac ggaggcgggc
      301 ggcgtctacg tcggctggcg gctgctgaaa caagacccgc ccgacatcgc cttcaacgtc
      361 taccgctcga ccgcgggcgg cgcggcggtg cgcctgaacg ccgcgccgat cacgaagacc
      421 accgacttcc tcgacgcgaa ggcgccgccc gaccgcgagc acgcgtggtg ggtgcgcgcg
      481 gtcgtcagcg gcacggagca ggagccttcg gtgcgcgcga cgctcgcggc aggctcgccc
      541 gcgcggcagt accgctcgtt caaaatccgc gacgacattc cggtgacttc gatcgacaag
      601 atcggcgtcg gcgacctcga cggcgacggc gagtacgact tcgtcgtcaa gcggcccggc
      661 ggcgagatcg accccagctt cctcacgcgc cacagccccg acaccttcaa gatcgaagcc
      721 tacaagagcg acggcacttt tctctggcgc agagacctcg gctggtcgat ccagctcggc
      781 atctggttct cgcccttcca cgtcgcggac ctcgacggcg acggcaaggc cgagatcgcc
      841 atcaagaccg gcgagggcga cccgcgcgac aagaacgggc aggtcttgaa ggggccggag
      901 tatctctcca tctgggacgg gatgaccggc aaggacatcg accgcacgga ctggataccg
      961 cgcggccagc tctcggactg gggcgactat tccggcaacc gctcgaaccg gcacatgctc
     1021 ggcgtcgcct acctcgacgg gaagaccccg agcgtggtca tcttccgcgg cacctacggg
     1081 ctgatgaggg ccgaggcgtt ttacttgaaa gataagaagc tgcacaaggt ctggagctgg
     1141 acgaacgagc gcgcgggctg gcgctatcag gggcagggcc agcacgccat ccacttcgcg
     1201 gacctcgacg gcgacggcgc cgacgaagtc ctcaacggct ccatcgccat cgagagcgac
     1261 ggtcgcatga tgtggagcac ggggatgggc cacggcgacc gcttctacgt gacggacatc
     1321 gacccggcgc ggcccggcct cgaaatctgg tacagctacg aagacccgca cccgcgcaac
     1381 ggcgtcagcc tgtgggacgc caagaccggc gacatcatct tcggcacgga cgaggagacc
     1441 gccgacaatc aggtcggcag cgcgctcgtc ggcgacatcg acccggccta tcccggcatg
     1501 gaagtctggg gcgagaagtt cttctacacc gcgaagggcg agcggattcc cggccccgtc
     1561 ccgccgcagc aggggcttgt ctggtgggac gcagaccccc tgcgcgagct gcacactttc
     1621 gggcgcggcg caggccgcgg ccccgccatc aaccccgcga ccggcgcccc cgctactgga
     1681 caggacacga cacagggcac cgcacagggc gggggacagg gcgcgcgccc cgcgggtcag
     1741 ggcggcccca acttcatgcg gggcagcacg gtctcgaagt ggaaggggtc ggtgctgacc
     1801 tcgaacatac aggggcggac ggtgctctgg ggcgacatcg cgggcgactg gcgcgaggag
     1861 atcgtcacct tcgcgcaggg cgaggtgcgc atctacggca ccgtcatccc ggccacggat
     1921 cgccgcgtca cgctcatgca agacccgctc taccgcagcg aggtcggcct cgtcgtccag
     1981 ggctacacgc acgtccccgt gacgagctac tacctcggcg tgaaataaaa gcgggacggg
     2041 cgggatttag gggcgttgac ggcaaggtgt catcgctttg caacataatt gcaaccaaag
     2101 ggggcggggc cgaggctact attgcttcac gtaacggcgt tccttctgag aacgagtgaa
     2161 actttggggc aggccacgac ctagtggcca aaggcgtcag gcttggggga gtctgacgcc
     2221 ttttcttttt ttcggcggcc cagaggggaa gagcagaggg gataagggga agaggggaaa
     2281 gggcgaaaag gaaagaagac aggcgacttg cgttgtcttc tcccttttct ccctttcccc
     2341 ttttccccct ttgtcttaca tccggtaggc gtcgagttcc tgaagatggg agggcatttc
     2401 ggggatggcg aggcagcgcg gtgaggtctg tgcggacgag ctttgccagg tcgatggccg
     2461 cgcggctgtt gagctggccg gcgccctgct cgaaggtgtt gaagccgcgg agctgctgcg
     2521 ccgtgtacat cagatcatct tgacgaggtt cggcgtgagc ttgggctgca tccgcaacat
     2581 cagcgccgcc gtgccggcga cgaccggggc cgacacggac gtgccgctca tgtacatcag
     2641 gcggctgttg gtcgagaagc cgacgttgac gccggggtcc atctgcgggt actcggtgat
     2701 gagcttgttg ccggtcgcct gcgcgctgat gagcttgttg ccgggggcga cgaggtcggg
     2761 tttgatgagg tggtcgtagt agcgcgtgcc ggaaacgtcc gtccagtagg agcgcgtcgg
     2821 cccgcgcgag ctgtaggtgg cgacggtgtc gtccgagccg gtgtccgtgc cgtaggtgtt
     2881 cgacgcgccg accgtgatgg ccgaaggctc gttgcccggc gagtgcacct gcccgtagac
     2941 cttctgcccc gcctcgtcct tgccgttgtt gcccgcggcg acgacgacca ccacgccggc
     3001 gtccgccatc ctccgcacgg ccttgcacgc cgggtcgttc ctgaacgaat cgaccgccgc
     3061 catgccgagg ctcatgtgtt gtgtgctccc atctcaagtt ggtagagctt gtgcgcgcca
     3121 ccatacagac caaacattga gaattttttg agaagcgacc gtcaggtgtg gagaatgcgt
     3181 gccgccggtc ggcgggctgg aaggcgacgg cggcgggttt ttatcgacgg cgcgcggggt
     3241 gttcttcgtc gggcgcttgc ttgcggatga cggcgacgta ggtggggccg tccgcgtcga
     3301 ggaacctttc gacgagccag ccggtgcctt ccagcacctc ttccatctcg cgccgcgaga
     3361 ccagcagata gtcgaaccag gggccgacgg ccctgccgaa ccgcacgcgc atcgtaagct
     3421 gcccggccaa ccgcccgcgg ctgaggttgc ggcggtggta gcgcaggtgc tccgggtcgt
     3481 cggtggcgtg cgggtcgagg ctctgcgcga tgatgcgggc gccgtccgtg gtgacgcgcg
     3541 cgagctttcg cagcgtccgg cgcgcgcccg cgcgcgagcc gaacaggccg aagttgttgc
     3601 ccagcatcag caccgtgtcg aacgagccgg gcgcgaacct gtccacctcg tctatcgaac
     3661 ggacgagcgc cttcttcaag ccgcgcatcc ggcacacgcg caccgcgccg ggcgaaagat
     3721 cgatgccggt cacgtcgagg ccgcgcgact gtaggtagag cgagtggcgg cccgcgccgc
     3781 agccgatgtc gagcacgcgg cccgcggcga gccggatggc gcggcgctcg gcgcgcgacc
     3841 agtctttgta ttcggagaag tagcgcgcgg ggtcttggcc cgcgccgacg tagccgtcgg
     3901 cgcgctcgat gatctcggcg ccttgcctcc ggcccgcgtg cagcgccagc agttgctggc
     3961 cgaaagcgtc cgacgccttc ctcatcctgc ggcccctttg cgaagtgctt cgacccgcgc
     4021 ccttacgtta cacttgacgg gcgaaccggg agggtcttct cttaagcgat gctctacaac
     4081 gtcaggcaat cttacacccc cgcgctcgaa gcgcacgagt tcaacccgca cgcgctcatc
     4141 atcgacacgg agacggtcgg caccggctcc tccatcgagg tcgtcgaggt cgcgctctgc
     4201 gacttcgagg gccgcgtcct gctcgacacg ctcgtgcgcc ccgtctacaa cccgccgccg
     4261 cgctccacgc ccgcgcagcg cttcgacagc acggagttcg acgccgcgcc cgactggacg
     4321 gacgtgtggc ccgcgctcgc cgcgcttgcc gagaacaagc tgctcgtcgc ctacaacgcc
     4381 gccttcgacc gccgcgcgct cgccgccatg tgcgcgcgca accgtttgcc ctcggaggcg
     4441 ggccgcggct ggcgctgcgc catgcagctc gccaagcgcg ttctcggcgc gaagaagaac
     4501 ctcctgctct ccgaggcgtg cgcgcgcttc ggcctcgaag cgggcagcca ccgcgccgcc
     4561 gcggacgtgc tcgccacgca ccgtctgctc aagcgcctcg tcacgcccga actctagaga
     4621 ccgtcggaca aaattcacca cgaagacacg aagggcacaa agaagaaaga taaaaagtaa
     4681 gacttacagc aaagtcgtcc ttattgtctt tgtcttcttt tgtgcccttc gtgtcttcgt
     4741 ggttaagttc tgtttgaccg tctcagttcg cgtcggcgac gacgcgtggt cgcgcgccga
     4801 gcgggtgcgc gcgctcgtag gcggaggcca aacggaagag cgtggcctcg ctccagtgcg
     4861 cgcccatgag ttggaagccg atggggagtc cttcggaaga gagcgcgcag ggcacgctga
     4921 tgccgggcac gcccgcgagg ttagccatgc aggtgtagat gtccgagagg tacatcgcca
     4981 gcgggtcgtc cgtcttctcg ccgaggcgga aggcgggcgt cggcgaggtc ggcgtgagga
     5041 tcgcgtcgca cttttcgaag gcccgcgcga agtcctgctt gatgagcgtg cgcacctgct
     5101 gcgcctggag gtagtaggcg tcgtagtagc cggacgagag gacgtaggtg ccgagcatga
     5161 tgcggcgctt gacctctgcg ccgaagccct cgtcgcgcgt gcggcggtac atctgccgca
     5221 gctccggcgc ctcctctgca cggtagccgt agcgcacgcc gtcgtagcgc gcgaggttcg
     5281 acgaagcctc ggcggtggcg atgatgtagt agacggcgat gctgtacttc gcgtggggca
     5341 gctccacgtc aacgatctcg gtgccgagtt cgcggtacgc ctcgatcgcc gcttcgaccc
     5401 ccgcgcgcac ctccgcgtcg agtccttcgc cgaacagcgc gcgcggcacg ccgacgcgcg
     5461 cgcccttgat gtcgcccgtc aacccggacg cgtaggcggg cacgggcacg tcggcggtcg
     5521 tcgcgtcgcg ctcgtcgcgc ccggcgatga cgccgagcac gtgcgccgcg tccggcaccg
     5581 tcagcccgaa gacgctcacc tggtcgagcg acgagccgaa ggcgaccagc ccgtagcgcg
     5641 agacgcgacc gtaggtcggc ttcgcgccga cgatgccgca gagcgccgcc ggttggcgca
     5701 ccgagccgcc cgtctcggag ccgagcgcga cgggcacgat gcgcgccgcg accgccgccg
     5761 ccgagccgcc cgaagagccg cccggcacgc gctccgtgtc ccaggggttc ctgacggggc
     5821 cgaacgccga attctcgttc gacgaaccca tcgcgaactc gtcgcagttc gtcttgccga
     5881 cgacgaccgc gcccgtcgcc ttcaggcgct cgacgacggt cgcgtcgtac ggcggctggt
     5941 agtcgccgag gatgcgcgac ccgcacgacg cctgcaagcc gcgcacgcag atgttgtctt
     6001 taatggcgat gggaatgccg cgcagcggcg aatcgggcgc ggacttgtcc agctcttcgg
     6061 cccgccggat cgcgcccgca cgatcaattt gcagaaaggc gttcagcgca tcgttcagct
     6121 tttccgccgc gccgactgcc gccgccgcga cggcgcgcgc cgtcgtctcg ccgccagaga
     6181 gtgcagacga aatgtgctcg attgttttct caggagctgt catggaaatt ttgatcccgt
     6241 ataaaaagct cggcgttagc gaagactgta ggggcaggga caagccctgc ccctacagtc
     6301 ttcgctaacg cgccgagttt agtgtcagct taaaaatctt ccttctcttc ggagccggcg
     6361 atgaccttcg gcacgcggaa gtgcccgtgc gaagggtcgg gcgcctcggc gagcgcaagc
     6421 tcctggccga gcgaggggcg cgtgcggtct tcgcgcacgg cctccgtgcg cccgccctcg
     6481 ggcgtgaggc cgccgatggc cggctccact tggctcgtgt ccagctcgtt caactgttcg
     6541 acgtaggcga cgattgccgc cacctgcggc ccgagatggc gcaactcctc gtcggtgatt
     6601 tccaggtgcg cgaggtctgc gatcttcctg atgtcggctt cggtaatcgg cataagtaaa
     6661 agccgtgaac cgtgaaccgt caaccgtggc aagtaagaac cggtttttta cttgtcacgg
     6721 ttgacggttc acggtttacg ttcttgggcg ttcaagtagc tgccggcggc gatgagaaag
     6781 cggcggcgca gggtttcgtc gcggatggcg gaggccgccg cccgcagctc gcccgagcgc
     6841 cgcagcgcgt ggtcttcgtc ggtcggcgtg ccggtttgca ggcgcaggcg gtcgcgctcg
     6901 gcctggacgg tgcgcgcgtc gatgcggaac tcgatgaagg tgacgagcgc ctgaccgagc
     6961 agcgagttga cgcggaagag cagttgcccg ctgacctttt cgagctgctt ctgccacgtc
     7021 tggtcgggga cggcgacgac gagggtcttg cggtagagcc ggaagggcac cgccgccgcg
     7081 cgcacgccct cgcccgccgc gcggcgccac gcgacgaagg cggcggcctc caagacctct
     7141 tcggcctcgc ccgccgcgcg cagcagcttc ggcaaagact ggatcagctc gtccatctct
     7201 cgaatgcgga ttgcggattt cggattgcgg atttatgggt cagaatttaa tctgaaatcc
     7261 gcaatccgaa atccgcaatc gagtgtgggg gagtgtaatg gaaaaagaga ggagcctaca
     7321 agccgggggc gcgcaagggg cgtggctggg ggcgctggtg ccgctggtgc tggcttcggg
     7381 cagcccgcgg cgcgcggaga tactgcgcgc ggtcgggtgg cccttcgagg cgagcgcacc
     7441 cgaagtggac gagtcgcgcc gcgagggcga gcgggcggag gactacgtcc gcaggctcgc
     7501 gcgcgaaaag gccgaggcgg gcgcctcgac gcgcctgttc gggctggtgc tcggcgcgga
     7561 cacggtggtc gtcgtcgaga gggaactcct cggcaagccg cgtgacgagg cggacgcgcg
     7621 caggatgctg cgcgccttga gcggtcgctg gcacgaagtc ctgacgggcg tggcgctggt
     7681 gcgcgcagag tccggacggg cgctggtcgg cgtcgagcgg acgcgcgtgc gtttcgcgca
     7741 gatgtcggac gaggagatcg actggcacgt ctcgacgggc gagccgctcg acaaggcggg
     7801 cgcctacgcc gtccaagggc gcgccgcgct cttcatcgag gccatcgagg gcgactactg
     7861 gaacgtcgtc ggcctgcccg tccggctcgt ctacgaactc gtgcgaaagc tcgaagagga
     7921 aaaggaagaa gggcgaaaag ggtaaaaggg taaagggcga aaagggaaga tgagaggcac
     7981 gctgttacac gtcctcaacc gaacatgaaa agcagcgtgc ctctcatcga gttgatgatg
     8041 agaggcacgc tgcttttgtc ttcccttttc gcccttcttt actgtccgcc gagcagcgtg
     8101 ttgagcctgc cggagaggac gttgacgagc ttgttcttgg ggtctgtgga gatgccgccg
     8161 acgggcgcgc cggcgacgcg gccctcgcgg tcgaggatga tgacggaggg aatctggtcg
     8221 gcgccggtct tcttgaagaa ggtaccgtcg gggtcgcgca gcacggcgac cttgaggccg
     8281 ttcttgcgcg ccagcgtgcg cagttgttcg tccgtcgcgt agttcttcga ctgcggcttg
     8341 tccgagtccg tgctcaccca gtagacgcgc acgttccgct gcgcgaactc gtccgacaac
     8401 tcctgcacgc cctgcatctg cgccttcgag agcgggagcc aggacgcgcc gaaggcgagc
     8461 acgaccacgt cgccgcgcag catccccgaa gagatttcgc cgccgtccgc cgtcttcaga
     8521 cggaaatcga cctgcgccgc cgcgcccgtc ggcagggcca acgcgaggct cgacagagag
     8581 aacaacagcg ccgccgtcaa ggccaaaagg gagcggcgtt taggggtcaa cattttcatc
     8641 tccttcgggc cgtgggtcag ttactgacgg cccgcgccgg agcgccgaaa tgcgaggcgc
     8701 tcatgccaat ctttgatgaa ctgtcaaaaa gaatggttgg tactgatggc gtacagcggc
     8761 gggtttattt tcgaacccga accattccca gcctactcga cggccccccg cggcgcgaac
     8821 ccgaaaacct cggcgtgcag tttacagtag tcctcccatg tgtccatgtc gcgcgtgacg
     8881 aaaggcgtcg cggcggggac gcgcaagac
//
LOCUS       DBNGOH010000618         7590 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_91668, whole genome shotgun sequence.
ACCESSION   DBNGOH010000618 DBNGOH010000000
VERSION     DBNGOH010000618.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7590)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7590)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7590
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_91668"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..846
                     /locus_tag="AC159P_12020"
     CDS             <1..846
                     /locus_tag="AC159P_12020"
                     /inference="COORDINATES: protein motif:HMM:NF016800.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008754 - O-antigen ligase activity
                     [Evidence IEA];
                     GO_function: GO:0008755 - O antigen polymerase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HIR3696216.1"
                     /translation="PLGLLFSGAIDRDRIPLYAFAALLCSVALVMTNSRGGIIAMVAE
                     ILFLVVIAAGVGRRAAGEGAQDLKSRVGAAAKRVAFAFALLATLFVFALYSGGEESLT
                     RLFGTINADDPTTGRAHFWKGAVEMIKEHPLLGTGLGAFSAVYTRHDTGSGMYRLEQA
                     HNDYLQILTDGGVVGAVLALAFVVVLFRQGFRRLQSRDKFRRGVALGALTGCAGALVH
                     SAFDFTLHTTANALLFLLLAALATMNGRVEEAAPPRRKRVRRRREGLIQQPPLPRAQG
                     TYDPL"
     gene            874..1830
                     /locus_tag="AC159P_12025"
     CDS             874..1830
                     /locus_tag="AC159P_12025"
                     /EC_number="4.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015208603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048040 - UDP-glucuronate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronic acid decarboxylase family
                     protein"
                     /protein_id="HIR3696217.1"
                     /translation="MRILVTGGAGFIGSHLCERLLAEGNDVLCLDNFFTGRRENIFHL
                     LDDKRFELARHDVIEPILLEVDQIYNLACPASPIHYQYNPVKTVKTNVLGTINMLGLA
                     KRVRARIFQASTSEIYGDPEVHPQQETYWGHVNPVGIRSCYDEGKRLAETLMMDYHRQ
                     NKVDIRIARIFNTYGPRMLENDGRVVSNFIVQALRGEQLTIYGDGSQTRSFCYVDDLV
                     EGFIRLMNAEEGAHPSVNVHEPVNLGNPGEFTMKELAEEVARIVGQPLEMRFCPLPAD
                     DPRQRRPDITRAKELLGWSPAVPLREGLRRTVEYFAGRIQKQ"
     gene            1906..3738
                     /locus_tag="AC159P_12030"
     CDS             1906..3738
                     /locus_tag="AC159P_12030"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015645 - fatty acid ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-dependent synthetase/ligase"
                     /protein_id="HIR3696218.1"
                     /translation="MAEHSKLETPSTIPGMCLSALRHYAKPDAVNLKSGGEWLHISGE
                     DFIRRIRAVALGLSALGVRKGDRVALLSENRPDWSVADLAILSLGAVNVPIYTTQAPE
                     QVRYILEDSGARVLFVSGKKVYRHARPGVEPVAGLEHVVFFDTKAEGERAGAMSLMEL
                     EQQGAEIDRLSPETFERALNEVRADDLATIIYTSGTTGEPKGVMLTHSNFVSNVLTIS
                     DSLPIRSTDIALSVLPLSHIFERTVFYVFCWNGVAVHYAASFDQVGEYLREVRPTIMT
                     AVPRLFEKVYHRIVKKGTSAGGYKEKLFMWALGVGQRYAALKDKGEEVPPLLEIQQEV
                     ASRLVFSKWREGVGGRLRFFVSGGAALSPALSYAFLGAGVNILQGYGMTETCIVAANR
                     PENNRVGSVGPAFPGVEVRTDAEGEILVRGPNVMRGYYNHPEDTAAVFTDDGWFKTGD
                     VGKVDEEGRLFITDRKKELFKLSNGKYVAPQLIESLIKQSSFVSQVVVVGAGRKSPAA
                     LVVPDWETLSAALPEPQAEEHTSRVSWSRNPAAVKLVQREVAGLTSTLADYERVRRVA
                     LLPDEFSIDGGELTPTLKVKRRVIDEKYGGLIDELYDGAAVKEG"
     gene            complement(3853..5685)
                     /locus_tag="AC159P_12035"
     CDS             complement(3853..5685)
                     /locus_tag="AC159P_12035"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015645 - fatty acid ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-dependent synthetase/ligase"
                     /protein_id="HIR3696219.1"
                     /translation="MAEHSKLETPSTIPGMCLSALRHYAKPDAVNLKSGGEWLHISGE
                     DFIRRIRAVALGLSALGVRKGDRVALLSENRPDWSVADLAILSLGAVNVPIYTTQAPE
                     QVRYILEDSGARVLFVSGKKVYRHARPGVEPVAGLEHVVFFDTKAEGERAGAMSLMEL
                     EQQGAEIDRLSPETFERALNEVRADDLATIIYTSGTTGEPKGVMLTHSNFVSNVLTIS
                     DSLPIRSTDIALSVLPLSHIFERTVFYVFCWNGVAVHYAASFDQVGEYLREVRPTIMT
                     AVPRLFEKVYHRIVKKGTSAGGYKEKLFMWALGVGQRYAALKDKGEEVPPLLEIQQEV
                     ASRLVFSKWREGVGGRLRFFVSGGAALSPALSYAFLGAGVNILQGYGMTETCIVAANR
                     PENNRVGSVGPAFPGVEVRTDAEGEILVRGPNVMRGYYNHPEDTAAVFTDDGWFKTGD
                     VGKVDEEGRLFITDRKKELFKLSNGKYVAPQLIESLIKQSSFVSQVVVVGAGRKSPAA
                     LVVPDWETLSAALPEPQAEEHTSRVSWSRNPAAVKLVQREVAGLTSTLADYERVRRVA
                     LLPDEFSIDGGELTPTLKVKRRVIDEKYGGLIDELYDGAAVKEG"
     gene            complement(5761..6717)
                     /locus_tag="AC159P_12040"
     CDS             complement(5761..6717)
                     /locus_tag="AC159P_12040"
                     /EC_number="4.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015208603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048040 - UDP-glucuronate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronic acid decarboxylase family
                     protein"
                     /protein_id="HIR3696220.1"
                     /translation="MRILVTGGAGFIGSHLCERLLAEGNDVLCLDNFFTGRRENIFHL
                     LDDKRFELARHDVIEPILLEVDQIYNLACPASPIHYQYNPVKTVKTNVLGTINMLGLA
                     KRVRARIFQASTSEIYGDPEVHPQQETYWGHVNPVGIRSCYDEGKRLAETLMMDYHRQ
                     NKVDIRIARIFNTYGPRMLENDGRVVSNFIVQALRGEQLTIYGDGSQTRSFCYVDDLV
                     EGFIRLMNAEEGAHPSVNVHEPVNLGNPGEFTMKELAEEVARIVGQPLEMRFCPLPAD
                     DPRQRRPDITRAKELLGWSPAVPLREGLRRTVEYFAGRIQKQ"
     gene            complement(6745..>7590)
                     /locus_tag="AC159P_12045"
     CDS             complement(6745..>7590)
                     /locus_tag="AC159P_12045"
                     /inference="COORDINATES: protein motif:HMM:NF016800.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008754 - O-antigen ligase activity
                     [Evidence IEA];
                     GO_function: GO:0008755 - O antigen polymerase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HIR3696221.1"
                     /translation="PLGLLFSGAIDRDRIPLYAFAALLCSVALVMTNSRGGIIAMVAE
                     ILFLVVIAAGVGRRAAGEGAQDLKSRVGAAAKRVAFAFALLATLFVFALYSGGEESLT
                     RLFGTINADDPTTGRAHFWKGAVEMIKEHPLLGTGLGAFSAVYTRHDTGSGMYRLEQA
                     HNDYLQILTDGGVVGAVLALAFVVVLFRQGFRRLQSRDKFRRGVALGALTGCAGALVH
                     SAFDFTLHTTANALLFLLLAALATMNGRVEEAAPPRRKRVRRRREGLIQQPPLPRAQG
                     TYDPL"
BASE COUNT         1263 a         2532 c         2532 g         1263 t
ORIGIN      
        1 cctttgggcc tgctcttctc gggcgcgata gaccgcgacc gcatcccgct ctacgccttc
       61 gcggcgctcc tgtgctccgt cgcgctcgtg atgaccaact cgcgcggcgg catcatcgcg
      121 atggtggcgg agatactttt cctcgtcgtc atcgcggcgg gcgtcgggcg gcgcgcggcg
      181 ggcgagggcg cgcaggactt gaagtcgcgc gtcggcgcgg cggccaagcg cgtcgccttc
      241 gcgttcgcgc tgctggcgac gctcttcgtc ttcgcgctct actcgggcgg cgaggagtcg
      301 ctgacgcgcc tcttcggcac catcaacgcc gacgacccga cgacgggccg cgcgcacttc
      361 tggaaaggcg cggtcgagat gatcaaggag cacccgctgc tcggcacggg actcggcgcc
      421 ttcagcgcgg tctacacgcg ccacgatacg gggagcggca tgtaccggct cgaacaggcg
      481 cacaacgact acctgcaaat cctgacggac ggcggcgtgg tcggcgccgt gctcgcgctc
      541 gccttcgtcg tggtgctctt ccggcagggc ttccggcggc ttcagtcgcg cgacaagttc
      601 cggcgcggcg tcgctctggg cgcgctgacg ggctgcgcgg gcgcgctcgt ccacagcgcc
      661 ttcgacttca cgctccacac gacggcgaac gcgctcctct ttctgctgct cgcggcgctc
      721 gccacgatga acggtcgcgt cgaagaggcc gcgccgccgc gccgcaagcg cgtccgccgc
      781 cgccgcgaag gcttgatcca acagccgccg ctgccgcgcg cgcaagggac ttacgacccc
      841 ctataacgat tcgaggaagg tattggcgga cagatgagaa tactggtcac gggcggcgcc
      901 ggcttcatcg gctcgcacct gtgcgagcgg ctgctcgcgg aaggcaacga cgttctctgc
      961 ctcgacaact tcttcaccgg aaggcgcgag aacatcttcc acctgctcga cgacaagcgc
     1021 ttcgagctgg cgcgccacga cgtgatcgag ccgatcctgt tggaggtcga tcagatttac
     1081 aacctcgcct gccccgcctc gccgatccac taccagtaca acccggtcaa gaccgtcaag
     1141 accaacgtgc tcggcaccat caacatgctc ggcctcgcca agcgggtgcg cgcgcgcatc
     1201 ttccaggctt cgacctccga aatctacggc gacccggaag tccacccgca gcaagagacc
     1261 tactggggcc acgtcaaccc cgtcggaatt cgcagttgtt acgacgaagg gaagcggctc
     1321 gccgagacgt tgatgatgga ctaccaccgg cagaacaagg tggacatccg catcgcgcgc
     1381 atcttcaaca cgtacggccc gcgcatgctc gagaacgacg ggcgcgtggt ctcgaacttc
     1441 atcgtgcagg cgttgcgcgg cgaacagctg accatctatg gcgacggcag ccagacgcgc
     1501 tccttctgtt acgtggatga tttagtcgaa ggctttattc ggttgatgaa cgcggaagaa
     1561 ggcgcgcacc cttcggtcaa cgtccacgag ccggtcaacc tcggcaaccc cggcgagttc
     1621 acgatgaagg agctggccga ggaggtggcg cgcatcgtcg gccagccgct ggagatgcgc
     1681 ttctgcccgc tgccggcgga cgacccgcgc cagcgccgcc cagacatcac gcgcgccaaa
     1741 gagctgctcg gctggtcgcc cgccgtcccc ctgcgcgagg gcctgcggcg caccgtcgaa
     1801 tacttcgcgg gaagaatcca aaagcagtaa atggtaaatg gtgaatagtg aatagaaact
     1861 gcttctcatc catttaccaa tcactattca ccattcactt ctcttatggc tgaacactcg
     1921 aagcttgaaa ccccatcgac gataccgggg atgtgcctgt cggcgctgcg ccactacgcg
     1981 aagccggacg cggtcaacct gaagtccggc ggcgagtggc tgcacatcag cggcgaggat
     2041 ttcatcaggc gcatcagggc ggtggcgctg gggctgtcgg ccctcggcgt gcgcaagggc
     2101 gaccgcgtgg cgctgctgtc ggagaaccgg cccgactggt cggtggccga tctcgccatc
     2161 ctcagcctcg gcgcggtgaa cgtgccgatc tacacgacgc aggcgcccga gcaggtgcgg
     2221 tacatcctcg aagactcggg ggcgcgcgtg ctcttcgtct cgggcaagaa ggtctaccgc
     2281 cacgcgcggc ccggcgtcga gccggtcgcc ggtcttgagc acgtcgtctt cttcgacacg
     2341 aaggccgaag gcgagcgcgc gggcgcgatg tcgctgatgg agcttgagca gcagggcgcg
     2401 gagatcgacc ggctctcgcc cgagacgttc gagcgcgcgt tgaacgaagt ccgcgccgac
     2461 gacctcgcca cgatcatcta cacgtcgggc acgacgggcg agccgaaagg cgtgatgctg
     2521 acgcactcga acttcgtctc gaacgtgctc acgatcagcg actcgttgcc catccgctcg
     2581 accgacatcg cgctctccgt gctgccgctc tcgcacatct tcgagcgcac ggtcttctac
     2641 gtcttctgct ggaacggcgt cgccgtccac tacgccgcct cgttcgatca ggtcggcgag
     2701 tacctgcgcg aggtgaggcc gacgatcatg acggcggtgc cgcgcctgtt cgagaaggtc
     2761 taccaccgca tcgtcaagaa gggcacgagc gcgggcggct acaaggagaa gctgttcatg
     2821 tgggcgctcg gcgtcgggca gcgctacgcg gcgctgaagg acaagggcga agaggtgccg
     2881 ccgctcctcg aaatccagca ggaggtcgcc agccgcctcg tcttctcgaa gtggcgcgag
     2941 ggcgtcggcg ggcggctgcg cttcttcgtc tcgggcggcg cggcgctctc gcctgcgctc
     3001 tcctacgcct tcctcggcgc gggcgtcaac atcctgcaag gctacggcat gacggagacc
     3061 tgcatcgtcg ccgcgaaccg gcccgagaac aaccgcgtcg gctcggtcgg gccggcgttc
     3121 cccggcgtcg aagtcaggac ggacgccgag ggcgaaatcc tcgtgcgcgg gccgaacgtc
     3181 atgcgcggct actacaacca cccggaagac accgccgccg tcttcaccga cgacggctgg
     3241 ttcaagaccg gcgacgtggg caaggtggac gaagaggggc gcctcttcat caccgaccgc
     3301 aagaaggagc tgttcaagct gtcgaacggc aagtacgtcg caccgcagct catcgagagt
     3361 ttgatcaagc agagttcgtt cgtcagccag gtggtcgtcg tcggcgcggg gcgcaaatca
     3421 ccggcggcgc tcgtcgtccc cgactgggag acgctctcag ccgcgctgcc cgagccgcaa
     3481 gccgaggagc acacgagccg cgtctcgtgg agccgcaacc cggcggcggt caagctcgtc
     3541 cagcgcgagg tggcgggcct cacctcgacg ctcgccgact acgagcgcgt gcgccgcgtc
     3601 gcgctgctgc ccgacgagtt ctccatcgac ggcggcgagc tgacgcccac gctcaaggtc
     3661 aagcgccgcg tcatcgacga gaagtacggc ggcctgatcg acgagctgta cgacggcgcg
     3721 gcggtgaaag aagggtaggc ggggcgaacg gatggcggag tgcgggttga aaggccgaag
     3781 gccgatggca gcagcgctgc tgccatcggc cttcggcctt tcaacccgca ctccgccatc
     3841 cgttcgcccc gcctaccctt ctttcaccgc cgcgccgtcg tacagctcgt cgatcaggcc
     3901 gccgtacttc tcgtcgatga cgcggcgctt gaccttgagc gtgggcgtca gctcgccgcc
     3961 gtcgatggag aactcgtcgg gcagcagcgc gacgcggcgc acgcgctcgt agtcggcgag
     4021 cgtcgaggtg aggcccgcca cctcgcgctg gacgagcttg accgccgccg ggttgcggct
     4081 ccacgagacg cggctcgtgt gctcctcggc ttgcggctcg ggcagcgcgg ctgagagcgt
     4141 ctcccagtcg gggacgacga gcgccgccgg tgatttgcgc cccgcgccga cgacgaccac
     4201 ctggctgacg aacgaactct gcttgatcaa actctcgatg agctgcggtg cgacgtactt
     4261 gccgttcgac agcttgaaca gctccttctt gcggtcggtg atgaagaggc gcccctcttc
     4321 gtccaccttg cccacgtcgc cggtcttgaa ccagccgtcg tcggtgaaga cggcggcggt
     4381 gtcttccggg tggttgtagt agccgcgcat gacgttcggc ccgcgcacga ggatttcgcc
     4441 ctcggcgtcc gtcctgactt cgacgccggg gaacgccggc ccgaccgagc cgacgcggtt
     4501 gttctcgggc cggttcgcgg cgacgatgca ggtctccgtc atgccgtagc cttgcaggat
     4561 gttgacgccc gcgccgagga aggcgtagga gagcgcaggc gagagcgccg cgccgcccga
     4621 gacgaagaag cgcagccgcc cgccgacgcc ctcgcgccac ttcgagaaga cgaggcggct
     4681 ggcgacctcc tgctggattt cgaggagcgg cggcacctct tcgcccttgt ccttcagcgc
     4741 cgcgtagcgc tgcccgacgc cgagcgccca catgaacagc ttctccttgt agccgcccgc
     4801 gctcgtgccc ttcttgacga tgcggtggta gaccttctcg aacaggcgcg gcaccgccgt
     4861 catgatcgtc ggcctcacct cgcgcaggta ctcgccgacc tgatcgaacg aggcggcgta
     4921 gtggacggcg acgccgttcc agcagaagac gtagaagacc gtgcgctcga agatgtgcga
     4981 gagcggcagc acggagagcg cgatgtcggt cgagcggatg ggcaacgagt cgctgatcgt
     5041 gagcacgttc gagacgaagt tcgagtgcgt cagcatcacg cctttcggct cgcccgtcgt
     5101 gcccgacgtg tagatgatcg tggcgaggtc gtcggcgcgg acttcgttca acgcgcgctc
     5161 gaacgtctcg ggcgagagcc ggtcgatctc cgcgccctgc tgctcaagct ccatcagcga
     5221 catcgcgccc gcgcgctcgc cttcggcctt cgtgtcgaag aagacgacgt gctcaagacc
     5281 ggcgaccggc tcgacgccgg gccgcgcgtg gcggtagacc ttcttgcccg agacgaagag
     5341 cacgcgcgcc cccgagtctt cgaggatgta ccgcacctgc tcgggcgcct gcgtcgtgta
     5401 gatcggcacg ttcaccgcgc cgaggctgag gatggcgaga tcggccaccg accagtcggg
     5461 ccggttctcc gacagcagcg ccacgcggtc gcccttgcgc acgccgaggg ccgacagccc
     5521 cagcgccacc gccctgatgc gcctgatgaa atcctcgccg ctgatgtgca gccactcgcc
     5581 gccggacttc aggttgaccg cgtccggctt cgcgtagtgg cgcagcgccg acaggcacat
     5641 ccccggtatc gtcgatgggg tttcaagctt cgagtgttca gccataagag aagtgaatgg
     5701 tgaatagtga ttggtaaatg gatgagaagc agtttctatt cactattcac catttaccat
     5761 ttactgcttt tggattcttc ccgcgaagta ttcgacggtg cgccgcaggc cctcgcgcag
     5821 ggggacggcg ggcgaccagc cgagcagctc tttggcgcgc gtgatgtctg ggcggcgctg
     5881 gcgcgggtcg tccgccggca gcgggcagaa gcgcatctcc agcggctggc cgacgatgcg
     5941 cgccacctcc tcggccagct ccttcatcgt gaactcgccg gggttgccga ggttgaccgg
     6001 ctcgtggacg ttgaccgaag ggtgcgcgcc ttcttccgcg ttcatcaacc gaataaagcc
     6061 ttcgactaaa tcatccacgt aacagaagga gcgcgtctgg ctgccgtcgc catagatggt
     6121 cagctgttcg ccgcgcaacg cctgcacgat gaagttcgag accacgcgcc cgtcgttctc
     6181 gagcatgcgc gggccgtacg tgttgaagat gcgcgcgatg cggatgtcca ccttgttctg
     6241 ccggtggtag tccatcatca acgtctcggc gagccgcttc ccttcgtcgt aacaactgcg
     6301 aattccgacg gggttgacgt ggccccagta ggtctcttgc tgcgggtgga cttccgggtc
     6361 gccgtagatt tcggaggtcg aagcctggaa gatgcgcgcg cgcacccgct tggcgaggcc
     6421 gagcatgttg atggtgccga gcacgttggt cttgacggtc ttgaccgggt tgtactggta
     6481 gtggatcggc gaggcggggc aggcgaggtt gtaaatctga tcgacctcca acaggatcgg
     6541 ctcgatcacg tcgtggcgcg ccagctcgaa gcgcttgtcg tcgagcaggt ggaagatgtt
     6601 ctcgcgcctt ccggtgaaga agttgtcgag gcagagaacg tcgttgcctt ccgcgagcag
     6661 ccgctcgcac aggtgcgagc cgatgaagcc ggcgccgccc gtgaccagta ttctcatctg
     6721 tccgccaata ccttcctcga atcgttatag ggggtcgtaa gtcccttgcg cgcgcggcag
     6781 cggcggctgt tggatcaagc cttcgcggcg gcggcggacg cgcttgcggc gcggcggcgc
     6841 ggcctcttcg acgcgaccgt tcatcgtggc gagcgccgcg agcagcagaa agaggagcgc
     6901 gttcgccgtc gtgtggagcg tgaagtcgaa ggcgctgtgg acgagcgcgc ccgcgcagcc
     6961 cgtcagcgcg cccagagcga cgccgcgccg gaacttgtcg cgcgactgaa gccgccggaa
     7021 gccctgccgg aagagcacca cgacgaaggc gagcgcgagc acggcgccga ccacgccgcc
     7081 gtccgtcagg atttgcaggt agtcgttgtg cgcctgttcg agccggtaca tgccgctccc
     7141 cgtatcgtgg cgcgtgtaga ccgcgctgaa ggcgccgagt cccgtgccga gcagcgggtg
     7201 ctccttgatc atctcgaccg cgcctttcca gaagtgcgcg cggcccgtcg tcgggtcgtc
     7261 ggcgttgatg gtgccgaaga ggcgcgtcag cgactcctcg ccgcccgagt agagcgcgaa
     7321 gacgaagagc gtcgccagca gcgcgaacgc gaaggcgacg cgcttggccg ccgcgccgac
     7381 gcgcgacttc aagtcctgcg cgccctcgcc cgccgcgcgc cgcccgacgc ccgccgcgat
     7441 gacgacgagg aaaagtatct ccgccaccat cgcgatgatg ccgccgcgcg agttggtcat
     7501 cacgagcgcg acggagcaca ggagcgccgc gaaggcgtag agcgggatgc ggtcgcggtc
     7561 tatcgcgccc gagaagagca ggcccaaagg
//
LOCUS       DBNGOH010000619         2088 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1925238, whole genome shotgun sequence.
ACCESSION   DBNGOH010000619 DBNGOH010000000
VERSION     DBNGOH010000619.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2088)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1925238"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1116)
                     /locus_tag="AC159P_12050"
     CDS             complement(<1..1116)
                     /locus_tag="AC159P_12050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015940913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HIR3696222.1"
                     /translation="MSVDTVPRKQIDAEQNAAWHSLTVEDSFARLDSMPRGLSAAEAA
                     RRLDEFGPNELQAAGRVSPWAILLEQFQNVLIIILLLATALSAFLGHGVEAIAITVIV
                     LFAVVLGFVQEYRAERAIEALREMAAPTATAIRDGRERKVPARELVPGDLVLLATGDK
                     VPADVRLVEAFNMQTVEAALTGESAPVEKHTRPLEDERLPTGDRKNMAYAGTAVTYGR
                     GRAVVVATGMATEFGKIARMLESVETGKTPLQENLDRVGKSLARAAFVVVLVIVALGL
                     FRGQPFVEMLIFGVALAVAVVPEALPAVVTISLALGVKRMVRRNALVRRLPAVETLGS
                     TSVICSDKTGTLTKDEMTARRLFVGGQTLEVSGTGYEP"
     gene            complement(1142..2023)
                     /locus_tag="AC159P_12055"
     CDS             complement(1142..2023)
                     /locus_tag="AC159P_12055"
                     /inference="COORDINATES: protein motif:HMM:NF012791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR3696223.1"
                     /translation="MKESAKILIAYDGSACSDAALNDLRRAGLPAAAEAVVITVADII
                     LPPPGDQLRDAEARVHHVMESERHAQAHAEQVLEEARAVARRAGKLLKAAFPDWQVQA
                     EVRCDSPAWAVVKLADQLDPCLVVVGSHGHSVAGGRLILGSVSQRVLYEARCSVRVAR
                     CSDERRAGPVRIVVGFNGSPDAEAAVEEVASRAWPEGSEAHVIVARAALKPEAPGVAV
                     ERLRAAGLTASEVDRDGDPAHVLIREAEEWGADSIFVGTRDLHGFQHLLHGSVSSAVA
                     ASAQCSVEVVRPSRGAA"
BASE COUNT          320 a          750 c          702 g          316 t
ORIGIN      
        1 cggctcgtag cccgtgcccg agacttcgag cgtctggccg ccgacgaaca ggcggcgggc
       61 cgtcatctcg tctttggtca gcgtgcccgt cttgtccgag cagatgacag aggtgcttcc
      121 caaagtctcg accgcgggca ggcggcgcac gagcgcgttg cgccggacca tccgcttgac
      181 gccgagggcg agcgagatgg tgacgaccgc cggcagcgct tcgggcacga cggcgaccgc
      241 gagcgccacg ccgaagatga gcatctcgac gaagggctgg ccgcggaaca gacccaacgc
      301 gacgatgacg aggacgacca cgaacgcggc gcgggccagc gacttgccga cccggtcgag
      361 gttctcctgc aacggcgtct tccccgtctc gaccgattcg agcatccgcg cgatcttgcc
      421 gaactcggtc gccatgcccg tggcgacgac gaccgcgcgc ccgcgcccgt aggtcacggc
      481 ggtgccggcg taggccatgt tcttccggtc gccggtgggc agccgttcgt cttcgagcgg
      541 gcgcgtgtgt ttctcgacgg gggccgactc gcccgtcagc gcggcctcca ccgtctgcat
      601 gttgaaggct tcgaccagcc tcacgtcggc gggcaccttg tcgccggtcg ccagcaggac
      661 gaggtcgccg gggacgagtt cgcgcgccgg aacttttcgt tcgcgcccgt cgcggatggc
      721 ggtcgccgtg ggcgcggcca tctcgcgcag ggcttcgatg gcgcgctcgg cgcggtactc
      781 ctgcacgaag ccgaggacga cggcgaagag cacgatgacg gtgatggcga tggcctcgac
      841 cccatggccg aggaaggccg acagagccgt cgccagcagc aggatgatga tgaggacgtt
      901 ctggaactgt tcgagcagga tcgcccacgg cgaaacgcgc ccggcggcct gaagctcgtt
      961 cgggccgaac tcgtcaaggc gtcgcgcggc ctccgcggcg ctcaggccgc gcggcatgga
     1021 gtccagcctc gcgaaactgt cttcgaccgt cagcgaatgc cacgccgcgt tttgttcggc
     1081 gtcgatctgt tttcggggaa cggtgtcaac cgacatgtat gttgtcctct tcgatctttg
     1141 ttcaggccgc acccctcgac gggcgcacca cctcgacgga gcactgcgcg ctcgcggcca
     1201 ccgccgagga gacgctgccg tgcaacaggt gttggaagcc gtgcaggtcg cgcgtgccga
     1261 cgaagatgga gtcggcgccc cactcttccg cctccctgat gaggacgtgc gccgggtcgc
     1321 catcccgatc gacctccgac gcggtcaacc ccgcggcgcg caacctctcc accgcgacgc
     1381 ccggtgcttc cggcttgagc gcggcgcgcg cgacaatgac gtgagcctcg ctgccctccg
     1441 gccacgcgcg cgaagcgacc tcttccacgg cggcttccgc gtccggcgag ccgttgaagc
     1501 cgacgacgat ccgcaccggc cccgcgcgcc gctcgtccga acagcgcgcc acgcgcacgg
     1561 agcaccgcgc ctcgtacagc accctctgcg acacgctgcc caagatgaga cggccacccg
     1621 cgacggagtg cccgtgcgaa ccgacgacga cgaggcaggg gtcgagctgg tcggccaact
     1681 tgaccaccgc ccacgcgggc gagtcgcagc gcacctcggc ctgtacctgc caatcgggga
     1741 aggctgcctt caacagcttc cccgcgcgcc gcgcgacggc ccgcgcctct tcgagcacct
     1801 gctccgcgtg cgcctgagcg tgccgctcgc tctccatgac gtgatgaacg cgcgcttccg
     1861 cgtcacgcag ttgatcgccc ggcggaggca ggatgatgtc ggccaccgtg atgaccacgg
     1921 cctcggcagc cgcgggcagc ccggcgcgcc ttaaatcatt gagcgccgcg tcggagcacg
     1981 cggagccgtc gtaagcaatc agtatcttcg cactctcttt catcttccac ctccatgcgg
     2041 cgagcagggg tcgttcatgc cttcgcgtaa acggcgcggg cggcgcga
//
LOCUS       DBNGOH010000620         6033 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3573947, whole genome shotgun sequence.
ACCESSION   DBNGOH010000620 DBNGOH010000000
VERSION     DBNGOH010000620.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6033)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6033)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6033
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3573947"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1261
                     /locus_tag="AC159P_12060"
     CDS             <1..1261
                     /locus_tag="AC159P_12060"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HIR3696224.1"
                     /translation="ITNKGFDFVKNDSDAKDELERGLLLFPGHGTGTSSVIVSPQGAQ
                     TNFGNLGPNPLAVWGVAPGAKLIPVRVSTSVVLGGGGVLNLARAIEHAADKSVHVISI
                     SMGTGFPNSRLLKAVQYAQKRGVIVLAAAGNFVGFVVWPAAYDEVIAVAASNARNKTW
                     QHSSRGSAVDVTAPGESVWCAGVEDNGGFSVGRGSGTSFAVATVAGMAALWLSHHGRD
                     KLAKRYGAEKIPVVFNKILRDTCDPLVGAGSGFGRGLVNAERMLAAPLPDGLQTPVPP
                     PAFALRDLPAIDSGGLATFRHLFETASTARRTRAATKATTSDTELRARLSELLGVGEE
                     ELPARLREVGQELAFHLASDPALYEAFAATLSPKRPRAAAKKGAAKGANPAAAAAIAP
                     VGVGAVRGALLLKDTSAALKTKVSGAG"
     gene            1387..3006
                     /locus_tag="AC159P_12065"
     CDS             1387..3006
                     /locus_tag="AC159P_12065"
                     /inference="COORDINATES: protein motif:HMM:NF013434.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR3696225.1"
                     /translation="MAGKIVEGRNLNPMQDWTLMPDFCVVGSGAAGGVCALKLAEAGF
                     DVIVLEEGPNIPKVDGKAPGQKRAMLNEREAEMYKLLYQEGGTRLTKNGGVKVMQGRC
                     LGGGTAVNWSACLPPRLETLDEWQKRGLPFTRQNLDKYLREVVNYLPIVRNDKYNTSA
                     RKFIEGCDHLGIPHDNLPNNTHNCRECGSCGVGCPYDRKMSGFVKWLPDAIAKGATVY
                     TDTKVDKLVGTADRVTEVHSHFIDGKNRPTGGTLVVKPTKGVVLAAGSIGTPGLLLRS
                     KLDAGKRVGRWTHIHPVTITIGKYPEKTLPAYGVPDNMWSPKFAAGPTGYLVETGSFF
                     PVLSASATLQHGPELHRILGDYYPHGAISYAHHTTGFDDKEPYGTVALDDNGDPELDY
                     VIPKANIKPMQESLEVMCRIHLAAGATSVYVMRNPPLEVRSEADFAEIRKINFAEAQR
                     STIFTVHVMGGCQMHKDDARRAVNNDFTFRGKKNLWVLDASIFPTALGANPQVTIYAL
                     ALWGAEEICKQHSAPFKLNHQQGGQWPWPGY"
     gene            2991..3521
                     /locus_tag="AC159P_12070"
     CDS             2991..3521
                     /locus_tag="AC159P_12070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696226.1"
                     /translation="MAGLLKTLNALADPKKRAEVKTGPLEHLNAKTALVLFAVAEAVL
                     GENFLQKAPKFIETVDDYLNYQRPAQREQFLVALLIAETRTFNLLVGGKPKGFSHMSL
                     AERRHVLERLRVSRQELNRNLYAAFVNVSASTYYASELTWPDLGYPGVSVDHPEILNQ
                     PPPVPWRPKDPRPIEP"
     gene            3573..4970
                     /locus_tag="AC159P_12075"
     CDS             3573..4970
                     /locus_tag="AC159P_12075"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HIR3696227.1"
                     /translation="MPDFFSEPFLHLAGLTHNSALITWGAFYFRVKDAGKEFKLVDDD
                     DLKRVFPPRRESIGARSEPYGPAKVTVSDMADNVVATASTSTANHCWVSGLAPDTEYK
                     YRVEVNGEVWARDERRDWVSGGDGAGLDLQGKSYDNRFRTFPDPARPAKPFHFAVIGD
                     FGVGIRKEKENRRQRRIAEALEKAVDEHDVRLVLTTGDNIYRTGGFLKIIGGQTGDED
                     DDWYFTYYQPYRYVINRVPVYPTIGNHDSPETEGKDDRSQLVDNFYMDERAAGALDAS
                     SLIRGLFYRFRFGSGVEFICLDTSKEGGPLSERVFKDQRNRGFLQSALPEASGEPRWR
                     IPFCHHPPFCAGPDHPSDGGGMRDLVKRFEDAGVRVVLSGHEHQFQHSRSNGVNYIVS
                     GASGKVNTGTPKKFAKARTVSWAAACHFLLVKVDGNRMTIRPVGELRDGKLADIELLA
                     PDGSAVTGPIVVNLA"
     gene            5020..5679
                     /locus_tag="AC159P_12080"
     CDS             5020..5679
                     /locus_tag="AC159P_12080"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3696228.1"
                     /translation="MSEKSDRYVPALGHDVLTPLYDAVVKWTTPESTFKRRLVRQANI
                     ERGQRILDLGCGTATLTLLLKQQHPDADVKGADGDPKILRIARAKAERAGAAVAFDHS
                     MAYALPYPDGSFDRVLSSLMFHHLTREDKSRTFREVRRVLRAGGELHFADFGKPHNAL
                     MYAASLPWRLFDGRATTRDNVKGELPPLLRDAGFAEVCETARYMTMFGTLSLFKTRKP
                     A"
     gene            complement(5712..>6033)
                     /locus_tag="AC159P_12085"
     CDS             complement(5712..>6033)
                     /locus_tag="AC159P_12085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007863461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696229.1"
                     /translation="ARITALYESLAELAPSPVVELNRAVALSMAYGPATGLELVEALK
                     EEPALKTYHLLPSVRGDLLAKLGRLEEARTEFERAASLTRNARERELLLRRARACAND
                     KIAG"
BASE COUNT         1084 a         2142 c         1903 g          904 t
ORIGIN      
        1 gattaccaac aagggcttcg acttcgtcaa gaacgactcg gacgcgaagg acgaactcga
       61 acgcggcctg cttctcttcc ccggccacgg caccggcacg tccagcgtca tcgtcagccc
      121 gcaaggcgcg cagaccaact tcggcaacct cggcccgaac ccgctcgccg tgtggggcgt
      181 cgcccccggc gcgaagctga tccccgtcag ggtctcgacc tcggtcgtgc tcggcggcgg
      241 cggcgtgctc aacctcgcgc gggccatcga gcacgccgcg gacaagagcg tccacgtcat
      301 ctcgatcagt atggggacgg gcttcccgaa ctcgcggctg ctgaaggccg tgcagtacgc
      361 gcagaagcgc ggggtgatcg tgctggccgc ggcgggcaac ttcgtcgggt tcgtcgtgtg
      421 gcccgccgcc tacgacgagg tgattgccgt cgcggcgagc aacgcgcgga acaagacttg
      481 gcagcactct tcgcgcggca gcgccgtgga cgtgaccgcg ccgggcgagt ctgtctggtg
      541 tgcgggcgtc gaggacaacg gcggcttctc ggtcgggcgc gggtcgggca cgtccttcgc
      601 ggtggcgacc gtggcgggca tggccgcgct ctggctctcg caccacgggc gcgacaagct
      661 cgccaagcgc tacggcgccg agaagattcc cgtcgtcttc aacaaaatcc tgcgcgacac
      721 ctgcgaccct ctggtcggcg caggctcagg cttcgggcgc gggctggtca acgccgagcg
      781 catgctcgcc gcgccgctgc cggacggtct gcaaacgccc gtgccgccgc cggccttcgc
      841 gctgcgtgat ctgcccgcca tagactccgg cggcctcgcc accttccgcc acctcttcga
      901 aacggcttcg accgcacgcc gcacacgcgc cgcgacaaaa gcgacaacgt cggacacgga
      961 gctgcgcgcg cgtctctccg aactcctcgg cgtcggcgaa gaggagctgc ccgcgcgtct
     1021 ccgcgaggtc gggcaggagt tggcctttca tctcgcctcg gaccccgcgc tctacgaagc
     1081 gttcgccgcc acgctctcac caaagcgacc gcgagccgcc gcgaagaaag gcgctgctaa
     1141 aggcgcgaat cccgccgccg cagcagcaat cgcgccggtc ggcgtcgggg ccgtacgcgg
     1201 cgcgctttta ttgaaggaca cttcggcggc gttgaagacg aaggtgtccg gcgcgggctg
     1261 aggagaaatg atgaacgatg aaggatgaat gatgaatgga agacacgtca tctttcatcc
     1321 ttcaaggcga ttcttactca tcgttcatca tgccgcattc atcatttcgg ttaaaggaga
     1381 cgagcgatgg cgggtaagat tgtcgagggc cgcaacctga acccgatgca ggattggacg
     1441 ctgatgcccg atttctgcgt cgtcgggtcg ggcgcggcgg gcggggtgtg cgcgctcaag
     1501 ctggccgagg ccgggttcga cgtgatcgtg ctcgaagagg ggccgaacat cccgaaggtg
     1561 gacggcaagg cgccggggca gaagcgcgcg atgctcaacg agcgcgaggc cgagatgtac
     1621 aagctgctct atcaggaggg cggcacacgg ctgacgaaga acggcggcgt caaggtgatg
     1681 cagggccgct gcctcggcgg cggcaccgcc gtcaactggt cggcctgcct gccgccgcgc
     1741 ctcgaaacgc tcgacgaatg gcagaagcgc ggtttgccgt tcacgcgaca gaacctcgac
     1801 aagtatctgc gcgaggtcgt caactacctg cccatcgtcc gcaacgacaa gtacaacacc
     1861 tccgcgcgca agttcatcga gggctgcgac cacctcggca tcccgcacga caacctgccg
     1921 aacaatacgc acaactgtcg cgagtgcggg agctgcggcg tcggctgtcc ttacgaccgc
     1981 aagatgagcg gcttcgtcaa gtggctgccg gacgcgatag cgaagggcgc gaccgtctac
     2041 accgacacga aggtcgataa gctggtcggc accgccgatc gcgtcaccga agtccactcc
     2101 cacttcatcg acggcaagaa caggccgacc gggggcacgc tcgtcgtcaa gccgaccaaa
     2161 ggcgtcgtgc tcgccgccgg ctccatcggc acgcccggcc tgctgctgcg ctcgaagctc
     2221 gacgcgggca agcgcgtggg ccgctggacg cacatccacc ccgtcaccat caccatcggc
     2281 aagtaccccg agaagacgct cccggcctac ggcgtgcccg acaacatgtg gtcgcccaag
     2341 ttcgccgccg gccccacggg ctacctcgtc gagaccggct cgttcttccc ggtgctttcg
     2401 gcctctgcca ccttgcagca cggcccggag ctgcaccgca tcctcggcga ctactacccg
     2461 cacggcgcca tctcctacgc gcaccacacg accggcttcg acgacaagga gccttacggc
     2521 accgtcgcgc tcgacgataa cggcgacccc gaactcgact acgtcatccc gaaggccaat
     2581 atcaagccga tgcaggagag cctcgaagtg atgtgccgca tccacctcgc cgcgggcgcc
     2641 acatccgtct acgtcatgcg caacccgccg ctcgaagtcc gaagcgaagc ggacttcgcg
     2701 gagatcagga agatcaactt cgccgaggcg cagcgctcga ccatcttcac cgtccacgtg
     2761 atgggcggct gccagatgca caaggacgac gcgcgccgcg cggtcaacaa cgacttcacg
     2821 ttcaggggca agaagaacct ctgggtcctg gacgcctcga tcttcccgac ggcgctcggc
     2881 gccaacccgc aggtgacgat ctacgcgctc gcgctgtggg gcgccgaaga gatttgcaaa
     2941 cagcacagcg cgccgttcaa gctcaaccac cagcaaggag gacaatggcc gtggccgggt
     3001 tactgaaaac gctgaacgcg ttagccgatc cgaagaagcg cgccgaggtc aagaccggcc
     3061 ctctcgaaca cctcaacgcg aagacggcgc tcgtcctctt cgccgtcgcc gaggccgtgc
     3121 tcggcgagaa ctttctacag aaggcgccga agttcatcga gaccgtggac gattacctca
     3181 actaccagcg cccggcgcag cgcgaacagt tcctcgtcgc gctgctcatc gccgagacgc
     3241 gcaccttcaa cctcctcgtc ggcggcaagc ccaaaggctt ctcgcacatg tctctggccg
     3301 agcgtcgcca cgtcctcgaa cgcctccgcg tgagcaggca ggagctgaac cgcaacctct
     3361 acgccgcctt cgtcaacgtc tcggcctcga cctactacgc cagcgaactc acttggcccg
     3421 acctcggcta tcccggcgtc agcgtcgatc acccggagat actcaaccag ccgccgcccg
     3481 tgccgtggcg gcccaaagac ccgcgcccca tcgaaccctg aagatgcccg gcgcccgccg
     3541 ggccgccacc ggacacgaga ggaggctccg tcatgcccga ctttttttcc gaacccttcc
     3601 tgcacctcgc gggcctcacc cacaactccg cgctcatcac ctggggcgcc ttctacttcc
     3661 gggtcaagga cgcgggcaag gagttcaagc tcgtggacga cgacgacctc aaacgcgtct
     3721 tcccgccgcg ccgcgagtcg atcggcgcga ggtcggagcc gtacggcccc gcgaaggtga
     3781 cggtctcgga catggcggac aacgtcgtcg ccacggcctc cacctcgacc gccaaccact
     3841 gctgggtgtc gggcctcgcg cccgacacgg agtacaagta ccgcgtcgag gtcaacggcg
     3901 aggtgtgggc gcgcgacgag cgccgcgact gggtgagcgg cggcgacggc gccgggcttg
     3961 accttcaggg gaagagctac gacaacagat tccgcacctt ccccgacccg gcccggcccg
     4021 ccaagccctt ccacttcgcc gtcatcggcg acttcggcgt cggcatcagg aaggagaagg
     4081 agaaccgccg ccagcgccgg atcgccgagg cgctcgaaaa ggcggtggac gagcacgacg
     4141 tgcggctcgt gctgacgacc ggcgacaaca tctaccgcac cggtgggttc ctgaaaatca
     4201 tcggcggcca gaccggcgac gaagacgacg actggtactt cacctactac cagccctatc
     4261 gctacgtcat caaccgcgtg cccgtctacc cgaccatcgg caaccacgac tcgccggaga
     4321 ccgaggggaa ggacgaccgc tcgcagctcg tcgataactt ctacatggac gagcgagccg
     4381 cgggcgcgct cgacgcgtcc tcgctcatcc gcggcctctt ctaccgcttc cgcttcggct
     4441 ccggcgtgga gttcatctgc ctcgacacgt cgaaagaggg cggccccttg agcgagcgtg
     4501 tgttcaaaga ccagcgcaac cgtggattcc tgcaatcggc cctgccggaa gcgagcggcg
     4561 agccgcgctg gcgcatcccc ttctgccacc acccgccctt ctgcgccggc ccggatcacc
     4621 cgagcgacgg cggcgggatg agagaccttg tcaagcgctt cgaggacgcg ggcgtgcgcg
     4681 tcgtgctgag cggccacgag caccagttcc agcacagccg gtcgaacggg gtcaactaca
     4741 tcgtgtccgg cgcatccggc aaggtcaaca ccggcacccc caagaagttc gccaaggcgc
     4801 gcaccgtctc gtgggccgcc gcgtgccact tcctgctcgt caaggtggac ggcaaccgga
     4861 tgaccatccg gcccgtcggc gagctgcgcg acggcaagct cgccgacatc gaactgctcg
     4921 cccccgacgg ctccgccgtc accggcccca tcgtcgtcaa cctcgcgtga ggcttgtcgc
     4981 ggcggggcgt cgagcacagg ataaggttat catcggcccg tgagtgagaa gtcagataga
     5041 tacgttccgg cgctcggcca cgacgtgctg acgccgctct atgacgctgt cgtcaagtgg
     5101 acgactcccg agtccacgtt caaacgccgc ctcgtccgac aggcgaacat cgagcgcggc
     5161 caacgcatcc tcgacctcgg ttgcggcacg gccacgctca cgctcctctt gaagcagcag
     5221 caccccgacg cggacgtgaa gggggcggac ggcgacccga agatactgcg catcgcacgg
     5281 gccaaggccg agcgggccgg ggccgccgtc gccttcgacc actcgatggc ctacgcgctg
     5341 ccctaccccg acggctcttt cgaccgcgtg ctgtcgagcc ttatgttcca ccacctcacg
     5401 cgcgaggata agtcgcgcac gttccgcgag gtgcgccgcg tgctgcgcgc gggcggcgag
     5461 ctacactttg ccgatttcgg caagcctcat aacgcgctca tgtacgcggc ctcgctgccg
     5521 tggcggctct tcgacgggcg cgcgacgacg cgcgacaacg tcaagggcga gctgcccccg
     5581 ctgctgcgcg acgcgggctt cgccgaggtc tgcgagacgg cgcgctacat gacgatgttc
     5641 ggcaccctct ctctcttcaa gacgcgaaag cccgcgtgag cgaaacaccg cgcgcaagct
     5701 ccgacctcta cttatccggc aatcttatcg ttcgcacacg cgcgggcgcg cctgagaagc
     5761 agctcgcgtt cgcgcgcgtt gcgcgtgagc gaggccgcgc gctcgaactc cgtgcgcgct
     5821 tcttcaaggc gcccgagctt ggcgagcagg tcgccgcgca cgctcggcag gaggtggtaa
     5881 gtcttgagcg ccggctcttc tttcaacgcc tcgacgagtt cgagtcccgt cgcggggccg
     5941 taggccatcg agagcgcgac cgcgcgattc agctccacga cgggcgacgg cgcgagttcg
     6001 gcgagcgact cgtagagcgc cgtgatgcgc gcc
//
LOCUS       DBNGOH010000621         6454 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_824231, whole genome shotgun sequence.
ACCESSION   DBNGOH010000621 DBNGOH010000000
VERSION     DBNGOH010000621.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6454)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6454)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6454
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_824231"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            964..1791
                     /locus_tag="AC159P_12090"
     CDS             964..1791
                     /locus_tag="AC159P_12090"
                     /inference="COORDINATES: protein motif:HMM:NF024242.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR3696230.1"
                     /translation="MSGTLLAVGPWRECGKIVVSAMTNRWSVLYTLLFEDIPTALGRM
                     RVDLILLSGCSTRRGWEGVAHGAWEICRDTPVVALGSNTGQDALVSALVGTAAPWPVE
                     GRSAGADDAGGGAADAGEAALPAEMHGRFRCSAVSPTLRLYEPQAKAAYSPGISRALD
                     LIEDRYAEPITLADAARAAAYSRCHFSKIFKEQLGMCFVRDLGRVRIRRAAELLARTE
                     MPVTSVALEVGFNDLSHFERVFRAVHKRSPTKFRAESKEMPHGEKYPPSLSTVAVTS"
     gene            2027..2731
                     /locus_tag="AC159P_12095"
     CDS             2027..2731
                     /locus_tag="AC159P_12095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696231.1"
                     /translation="MSKAHPTPRSARKSEAARAASAFADRIDVGAMAAGGMLFGGDAG
                     HRVHRAIHQRLRRLPKKGVLLVDFGGVRQASNQALKEALSVVDVLRSSKFEERYVVFR
                     VDTRNRDLVDSIEMVARLGGDIIPVIDRAGRRHNFGKLTKAERDTLKAVERFEELTSR
                     ELREGLGLLPSAASNRLRRLHHLRLIRRDERVVPASGGREFVYRPLVAGAGARRRKKK
                     RSAAAKERGAGRKRGR"
     gene            2755..3345
                     /locus_tag="AC159P_12100"
     CDS             2755..3345
                     /locus_tag="AC159P_12100"
                     /inference="COORDINATES: protein motif:HMM:NF025431.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Pvc16 family protein"
                     /protein_id="HIR3696232.1"
                     /translation="MADFHAIQVTCDAVMRLLEESYQPELLGPTNPLQFEVYGTTDFQ
                     SHMTQGVSLFLYRVHVNPNQRAPLVNNPAGGVRRQLLPLDLHFFITVWAPEPSLQHAI
                     LGWTMRTLEDNPVLTAPLLNGVRDNVFRADETVELLVGQLTNEELMRIWDDLNTDYHL
                     SVPYVARVVRIESLQTVSEGEPVTRREFEYGVLKGV"
     gene            3426..4397
                     /locus_tag="AC159P_12105"
     CDS             3426..4397
                     /locus_tag="AC159P_12105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696233.1"
                     /translation="MRPIYFPLETVTRTVPLGLRCVDIATGAQVAGGLSVTATPRERP
                     ARPARAALTPSGVYALHNLPGLVELEFADEAPSPASPPASPPGSEFVVVVEDFESRYL
                     PYAMTLTLPRRSLLTTFLFSVPSRGGATGLMSIRGSLKDSTRTLADGSLRPAAFARVV
                     AEYETTSPPTIFVGLADWRGEFALFMPGPNPLQPPEGVAVTSPNAPGRQTIAQLRWPV
                     TLGFFYEPQAQQFVTADERGRVEIIEGQQDGLTDVAPDSARTRYFPVLASLLNQSDAS
                     VFEVAGASGPPAAALAAEIEYGKDLVVRTREAPDSSVWLVPPAVVSP"
     gene            4419..6380
                     /locus_tag="AC159P_12110"
     CDS             4419..6380
                     /locus_tag="AC159P_12110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007670676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath family protein"
                     /protein_id="HIR3696234.1"
                     /translation="MPEYLAPGVYVEEVSYRAKSIEGVSTTVTGFIGPTRYGPVGGEP
                     ELLTSFADFERIYGGIDSLVFEPSGTGDEETQQNFMAHAVRAFFDNGGTKLYVTRVYE
                     YDDDPDEDTGKAGAASTSPAGVIFARFPGSGGNMRITMTVKVGPNVLNDDGANNATVS
                     GLTDHDLVYIRQHNLGSPSGAGPTGTAVVAGLYDVSRDDAGVVTFDNSSVGTPVQLGD
                     FDLPANYGVHLVTVIVSVRRPGRFTFDELIGEFSLSPSSRNSLSAYFTDDPESRNRFL
                     HVPFAIRPDNIGAGSEPTGSELFDALFGPGIADVLSRQTATDAELEFPSPAEERPSAS
                     ELTATITLAGGSDGRLPTANSYRGTDTGPLMTGLFTFEGVEEISIVAAPGAAYGSEQL
                     DASEDDRRRSQITSHLLTHVEKMKYRVAVLDSPNDAALSEVRNFRGKFDSKHAALYYP
                     WVTVLDPNDPDGIREIDLPPSGFVAGIYARNDVEHGVFKAPANEVVRGAIGFETLLNK
                     AQQDVLNPEGINCFRFFEGRGFRLWGARTISSDPEWKYISVRRYFAYLEHSVDRGTQW
                     AVFENNNDALWANIRRTVEDFLFNEWRSGALLGTKPEEAFFVRCDRSTMTQNDLDNGR
                     LICLVGVAVVKPAEFVIFRIGQWTADRKV"
BASE COUNT         1059 a         2263 c         2112 g         1020 t
ORIGIN      
        1 ggcggcgacc agatcgactt tgacgtggaa cgtacacgaa gactgcgggg caggaatcgc
       61 cccgccccca caacagtttt agcgaacgtc ttccaacgtt tcgaacgtgg cgtcgaaggg
      121 ggcggcgcgt gttttcgaaa acccgcgaat aacaaaaaaa atgtagttga caactccgca
      181 cgatgagtct atcctccgcc ctgcttttaa ttcactttca tgcctcgcct tcaaaggctg
      241 ttgccccgcg ctcggtagac cgtcgacgcc tctcgacacg ttgactcacc ggttgaaccc
      301 ttacggtatt cccatgatcc tgtgtccgct tcaccgcgaa gtctaccccg cgcgggacgc
      361 ctcggcgtcg cggggccggc ggtgcgcgcg cggcgagcac gtcaggagcc tgctgaattt
      421 caccatttga aaagagaagc cccgccgcta tcggcaaata gcggcggggt tcgagacaat
      481 gtaactgccg ccgcacgaat gcgccggggt acatcgcccg cagctacgta tcctaacgta
      541 aagtgcggtg ctggctcaac ttccagtgca ggagagtcag tcccgtgacg cacttcgtct
      601 catcccgccc gaacccggtc ggcctcgctc cgcccggcct tcgccttaac cgccggatga
      661 agttcccgtg cctcacgcgc cttcgcaaac ggaggacagg cgtcgcgcct cggacgcgca
      721 cgcccctccg gacgcctgcg cgaagaacaa ccccgcttta gtttcggagg gcagccgccg
      781 aggtggccgc cgcgtctttc gacgacgcgc ggccacttcg gcgacggggg agccatcgcc
      841 ctccaacgtg tttaccaaaa cacactaaac gggtctcgca tgttgggccg acggcagttt
      901 caaacccgcc ggggttttgc acgagccctc cggccattga aaccgtgcgg aggtctattt
      961 tccatgtccg gaactctgtt ggccgtcggc ccgtggcgcg agtgcgggaa gatcgtcgtg
     1021 tcggcgatga cgaaccgctg gagcgtgctc tacacgctgc tcttcgaaga catcccgacg
     1081 gcgctcgggc ggatgcgggt tgacctgatc ctcctctccg gctgctcgac gcgccgcggc
     1141 tgggagggcg tcgcgcacgg cgcgtgggaa atctgccggg acacgccggt cgtcgcgctc
     1201 ggctcgaaca cgggacagga cgcgctcgtc agcgcgctcg tcgggacggc ggcgccgtgg
     1261 cccgtcgagg ggaggtcggc gggcgcggac gacgcgggcg ggggggcggc ggacgcgggc
     1321 gaggcggcgc tgcccgccga gatgcacggt cggttccgct gctcggcggt gtcgccgacg
     1381 ctgcggcttt acgagccgca ggcgaaagcc gcctacagcc ccggcatcag ccgcgcgctc
     1441 gatctcatcg aagaccgcta cgccgagccg atcacgctcg ccgacgcggc gcgcgccgcc
     1501 gcctacagcc gctgccactt cagcaaaata ttcaaagaac aactcgggat gtgcttcgtg
     1561 cgcgacctcg ggcgcgtgcg catccgccgc gccgccgagc tgctcgcgcg caccgagatg
     1621 cccgtgacga gcgtcgcgct cgaagtcggc ttcaacgacc tctcgcactt cgagcgcgtg
     1681 ttccgcgccg tccacaaacg ctcgcccacg aagttccgcg cggaatcgaa agaaatgccg
     1741 cacggcgaaa aatatccgcc aagtctttcc acggtggccg tgacatcata gccgccgctc
     1801 tcggtcaacg ggtggttcga cacgggtcgc gggcgccgcg gctggccggt cgaacccgtc
     1861 tcgccggctc tcggcctcga cggcaagcgg gggcgtcctc gcgtgtcagc ggtctcgcgc
     1921 gctttcaccg tccacaacac ccgacggcgt tcgatgctct gccagaggtt cgatgctctc
     1981 cgagatcaat gtcagcccga caatctccgc gaacaggccc ggcagagtgt ctaaagcgca
     2041 ccccactcct cgcagtgctc ggaagagcga ggccgcgcgc gcggcgtccg ccttcgcgga
     2101 caggatcgat gtcggggcga tggcggcggg cgggatgctc ttcggcggcg acgccgggca
     2161 cagggttcac cgcgcgatcc accagcggct ccggcgcctg ccgaagaagg gcgtgctgct
     2221 cgtcgatttc ggcggcgtgc ggcaggcgag caatcaggct ttgaaggagg cgctgtcggt
     2281 cgtggacgtg ctgcgctcct cgaagttcga ggagcgctac gtcgtcttcc gcgtggacac
     2341 gcgcaaccgc gacctcgtcg attccatcga gatggtggcg cggctcggcg gcgacatcat
     2401 cccggtcatc gaccgcgcgg ggcgccgcca caacttcggc aagctgacga aggccgagcg
     2461 cgacaccttg aaggcggtcg agcggttcga ggagttgacc tcgcgggagc tgcgcgaggg
     2521 gctggggctg ctgccgagcg cggcgagcaa ccggctgcgg cggctccacc acctgcgcct
     2581 gatccggcgc gacgagcgcg tcgtgcccgc gagcggcggg cgcgagttcg tctaccgccc
     2641 gctcgtcgcg ggcgcggggg cgcggcggcg gaagaagaag cgaagcgcgg cggcgaaaga
     2701 gcgcggcgcg ggccgtaaac gcggacggtg acgtaaggag cggggcgaaa agcgttggct
     2761 gactttcacg cgatacaggt gacgtgcgac gcggtgatgc ggctgctcga agagtcctac
     2821 cagccggagc tgctggggcc gacgaaccct ttgcagttcg aggtctacgg caccaccgac
     2881 ttccagagcc acatgacgca gggcgtgagc ctcttcctct accgcgtcca cgtcaacccg
     2941 aaccagcgcg cgccgctcgt caataacccc gcggggggcg tgcgccgcca actgctgccg
     3001 ctcgacctgc acttcttcat cacggtctgg gcgccggagc cttcgctcca gcacgcgatc
     3061 ctcggctgga caatgcggac gctggaagac aaccccgtcc tgacggcccc gctgctgaac
     3121 ggcgtgcgcg acaacgtctt ccgcgcggac gagaccgtgg agctgctcgt cggccagttg
     3181 accaacgagg agctgatgcg catctgggac gacctcaaca cggattacca tctctcggtc
     3241 ccttacgtcg cgcgcgtggt gcggatcgag tccttgcaga ccgtgtccga gggcgagccg
     3301 gtcacgcggc gcgagttcga gtacggcgtg ctgaaggggg tttgaaggct ttccaaagaa
     3361 cgcggccgcc gaaacttcgc acggcccgtg accgggcgag cagcataaag ggtgacagaa
     3421 agagcgtgag gccgatctat ttcccacttg agacggtgac gcggacggtg ccgctgggcc
     3481 tgcgctgcgt ggacatcgcg accggggcgc aggtggcggg cggcctctcc gtcaccgcga
     3541 cgccgcgcga gcggcccgcg cgccccgccc gcgccgcgct gacgccgtcg ggcgtctacg
     3601 cgctgcacaa cctgcccggc ctcgtcgagc ttgagttcgc ggacgaagcg ccgtcgcccg
     3661 ccagcccgcc cgcctcgccg cccggcagcg agttcgtcgt cgtcgtcgaa gacttcgagt
     3721 cgcgctacct gccttacgcg atgacgctca cgctgccgcg ccggagcttg ctgacgacgt
     3781 tcctcttctc tgtgccctcg cgcggcggcg cgaccgggct gatgtcgatt cgcggcagct
     3841 tgaaagacag cacgcgcaca ctcgcggacg gaagtttacg gcccgcggcc ttcgcgcgcg
     3901 tcgtcgccga gtacgagacg accagcccgc cgacgatctt cgtcgggctg gcggactggc
     3961 gcggcgagtt cgcgctcttc atgccggggc cgaacccttt gcagccgccc gagggcgtcg
     4021 ccgtcacgtc gccgaacgcg cccggcaggc agaccatcgc gcagttgcgc tggccggtga
     4081 cgctcggttt tttctacgag ccgcaggcgc agcagttcgt cacggcggac gagcgggggc
     4141 gggtcgagat catcgagggg cagcaggacg gcttgacgga cgtggcgccc gactccgcgc
     4201 gcacgcgcta cttccccgtc ctcgcctcgc tcttgaacca gtccgacgcc tcggtcttcg
     4261 aggtcgcggg cgcttcgggg ccgcccgcgg cggcgctcgc tgcggaaatc gaatacggca
     4321 aagacttggt ggtacgaacc cgtgaggcac cggattcgag cgtgtggctc gtgccgccgg
     4381 cagtcgtctc accctaacac cgaaagagag gtctacacat gccagagtat cttgcgccag
     4441 gtgtgtacgt ggaagaggtc agctaccgcg ccaaatccat cgagggcgtc agcacgaccg
     4501 tcaccggttt catcgggccg acgcgctacg gcccggtcgg gggcgagccg gaactgctca
     4561 ccagcttcgc cgactttgaa cgcatctacg gcggcatcga ctcgctcgtc ttcgagccgt
     4621 cgggcaccgg cgacgaggag acgcagcaga acttcatggc ccacgccgtg cgcgccttct
     4681 tcgacaacgg cggcaccaaa ctctacgtga cgcgcgtcta cgagtacgac gacgacccgg
     4741 acgaggacac gggcaaggcc ggcgccgcga gcacctcgcc cgcgggcgtc atcttcgcgc
     4801 gcttcccggg cagcggcggc aacatgcgca tcaccatgac cgtcaaggtc gggccgaacg
     4861 tgttgaacga cgacggcgcg aacaacgcca cggtctcggg gctgaccgac cacgacctcg
     4921 tctacatccg ccagcacaac ctcggctcgc cctcgggcgc cggcccgacc ggcaccgccg
     4981 tcgtcgccgg cctctacgac gtgagccgcg acgacgcggg cgtcgtcacc ttcgacaaca
     5041 gctctgtcgg cacgcccgtc cagctcggcg acttcgacct gccggccaac tacggcgtcc
     5101 acctcgtcac cgtcatcgtc agcgtgcgcc gaccgggccg cttcaccttc gacgagctga
     5161 tcggcgagtt ctcgctcagc ccttcgagcc gcaactcgct ctcggcctac ttcaccgacg
     5221 accccgagtc gcgcaaccgc ttcctgcacg tccccttcgc catccgcccc gacaacatcg
     5281 gcgccggctc cgagccgacc ggctccgagc tgttcgacgc gctcttcggc cccggcatcg
     5341 ccgacgtgct cagccgccag acggcgaccg acgccgagct ggaattcccc agccccgccg
     5401 aggagcggcc cagcgcctcg gagctgaccg ccaccatcac gctcgcgggc ggcagcgacg
     5461 ggcggctccc cacggccaac agctaccgcg gcacggacac cggccccttg atgaccgggc
     5521 tgttcacctt cgagggcgtc gaggaaatct ccatcgtcgc cgcgcccggc gccgcctacg
     5581 gctccgagca gttggacgcc tccgaggacg accggcgccg gagccagatc acctcgcacc
     5641 tgctgacgca cgtcgagaag atgaagtacc gcgtggcggt gctcgactcg ccgaacgacg
     5701 cggcgctctc cgaggtccgg aacttccgcg gcaagttcga ctcgaagcac gccgcgctct
     5761 actacccgtg ggtgacggtg ctcgacccga acgacccgga cggaatccgc gagatcgacc
     5821 ttccgccctc gggcttcgtt gccggcatct acgcgcgcaa cgacgtggag cacggcgtct
     5881 tcaaggcccc ggccaacgag gtggtgcgcg gcgccatcgg cttcgagacg ctgctcaaca
     5941 aagcgcagca ggacgttttg aaccccgagg gcatcaactg cttccgcttc ttcgaggggc
     6001 ggggttttcg cctctggggc gcccgcacga tcagctccga ccccgagtgg aagtacatct
     6061 cggtgcgccg ctacttcgcc tacctcgaac actccgtcga taggggcacg cagtgggccg
     6121 tcttcgagaa caacaacgac gcgctgtggg ccaacatccg ccgcaccgtc gaggacttcc
     6181 tcttcaacga gtggcggagc ggcgccttgc tggggacgaa gcccgaggag gctttcttcg
     6241 tccgctgcga ccgctcgacg atgacgcaga acgacctcga caacggtcgc ctgatctgcc
     6301 tcgtcggcgt cgccgtcgtc aagcccgccg agttcgtcat cttccgcatc ggccagtgga
     6361 cggcggatcg caaggtctaa ggagcgatta gctgttagcc attagctatt agcttttggg
     6421 tctttcagct ttcggctttc ggtcgtcggc aaga
//
LOCUS       DBNGOH010000622        10719 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4123802, whole genome shotgun sequence.
ACCESSION   DBNGOH010000622 DBNGOH010000000
VERSION     DBNGOH010000622.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 10719)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10719)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10719
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4123802"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..688
                     /locus_tag="AC159P_12115"
     CDS             <1..688
                     /locus_tag="AC159P_12115"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: protein motif:HMM:NF012998.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HIR3696235.1"
                     /translation="ALAPVENTLAGSVHRSYDLLLDSSLRIAAEVVIPIRHFLIGCPG
                     STLEQVETVESHPVALAQCERFFAEHAHVRRVVAEDTAGSVAAVVGRGDPTRAAIAGK
                     RAADIYGGQVLREGLEDHRENYTRFVLLTPSDEVAAGADKLSLVVKLPHRPGALHHAL
                     EPFARRRIDLLKIESRPLRGQPWEYHFYLDLKASHEDAEAAAALAELHERAVSVRLLG
                     CYPSARAPLA"
     gene            921..1886
                     /locus_tag="AC159P_12120"
     CDS             921..1886
                     /locus_tag="AC159P_12120"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014234.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HIR3696236.1"
                     /translation="MLTDNGWERVTVVGCGLIGASFALALRRAGACARVAGWDASPSV
                     LDEALARGIIDEVDYAIADGGVSASDLVYLATPVGAIIEFLRERASQIKPRAVVTDAG
                     STKREVCRAARESLPRDRRFVGGHPVAGSHLRGPAHARADLFEAAPYVLTIDENERDR
                     DEVGGHGSGGDEGERRTREAFAETLRRTGARVTLMTAREHDRAMALVSHLPQLVSSTL
                     AATVESQPDGAALGRVAGAGFGDMTRLAASEWAVWRDILETNPSEVAAALDTFLLKLA
                     MVRDELRDCCAGDRDGLRVTSSLFNHSRTTGSRFEQSQPTSAVKG"
     gene            1896..3032
                     /locus_tag="AC159P_12125"
     CDS             1896..3032
                     /locus_tag="AC159P_12125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HIR3696237.1"
                     /translation="MILILTPNIDTEGETYRRLMGHLSRLPNIQARVHREQGVEQCLT
                     EIYLIGNTAAVSLNDMKNLPGVERAVRVSEEYRVIGRHKGDERPTHFEYQGVRFGQDT
                     LNVFAGLCAVDEPRHVELMMKALRDNGQVCTRMGAYKPRTSPYTFQGHGRACLTYVFE
                     LAGKYGIKVIAMEITHESHIAEIRDALDLTGSPTGVMLQIGTRNTQNFELLKIVGRQH
                     EFPVLLKRGFGITLDESLHAAEYLASEGNRKVVFGLRGMKTSIGDPHRNFVDFAHVPV
                     VKRLTRMPVCIDPSHSVGTRARCTEGILDIMHVTAQGVIAGANMVLVDFHPAPELALV
                     DGPQALRLEELPLFLEDVAIAREAYLKRLALAARDRRTEELAVA"
     gene            3262..3822
                     /locus_tag="AC159P_12130"
     CDS             3262..3822
                     /locus_tag="AC159P_12130"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3696238.1"
                     /translation="MFKHDLGAGAELRVLEARHAAEFLQFVDENRVFLGRWLRWGEEI
                     MTVAEAETFLRRGVKTYVDDGLPWVGIWLEGKMVGGVLFFPVEHRIAASEVGYWLGEG
                     ATGRGLVTRAVAAMLDYAFGTLGLHRMALLAEVGNEKSLAVARRLGFTEEGLKRAGGV
                     NRGKYVDLVAFSLLAEEWAARSAPTS"
     gene            4321..5544
                     /locus_tag="AC159P_12135"
     CDS             4321..5544
                     /locus_tag="AC159P_12135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011959141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trans-sulfuration enzyme family protein"
                     /protein_id="HIR3696239.1"
                     /translation="MSRVKDGHDITTQLVHAGERLPPPVGLPVSTPVYATATFTYDSM
                     AEVDQVFSGEKQGYVYTRYGNPTTAALEEAMRVLEGGAAACAYASGMAALHAALFACE
                     LAPGATVLASQDLYGATTDLLYNIFGSFGVNTVTADFTNTEALREKAREVRPRVLVAE
                     TISNPLLKVCDIEACAEVAREAGARFVVDNTFASPYLCQPLRHGADFAVHSATKYLGG
                     HADVMGGLVVARDEVDRAALVGVMKLVGGVLGVWEAHEVMRGLKTLAVRLDRQCDNAH
                     RLAERLDGHPRLARVHYPALDPSTETREVLGRVLRAPHAGALVSIELAEDTFDAAFRF
                     MDALDLCVRATSLGDVFTGVLHPATASHREVAPARRKRLGIADGLVRISVGIEDIGDI
                     IADIEQALKAVNREP"
     gene            5606..6544
                     /locus_tag="AC159P_12140"
     CDS             5606..6544
                     /locus_tag="AC159P_12140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011404767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HIR3696240.1"
                     /translation="MTEALLEEPLVVAPERGGLLSLVGGTPLLRLRRVARDVPAGVEV
                     WAKAEHLNPGGSVKDRAALAMVLEGEREGRLTPGKTILDATSGNTGIAYAMIGAARGY
                     AVALCLPRNAGAGRKRILRAYGAELVETDPTRSTDGAQLVAREMAARAPDKYFYPDQY
                     NNPANWRAHYESTAPEIREQTGARVTHFVAGLGTTGTFVGTSRRLKEYDPRVRAVAVQ
                     PDSPLHGLEGLKHLATAIVPGIYDASLADEHATVSTEDAQEMRRRLAHEEGLLVGTSS
                     GANVFAALRLARTLPGGSVVVTVLCDGGERYLGEEF"
     gene            6576..7028
                     /locus_tag="AC159P_12145"
     CDS             6576..7028
                     /locus_tag="AC159P_12145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014101325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008235 - metalloexopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HIR3696241.1"
                     /translation="MLSLTQAHEAEMRGHGERDYPYECCGLLVGRFAEGGGKEVVETY
                     PISNAREEEAKRNRFLIRPEELLRGERHARARGLDVVGFYHSHPDDEAIPSRYDLEHA
                     WPTYSYIVVSVREGRAVALRSWEMEADRSKFNEEEIRAVNREPSTVKS"
     gene            7091..8578
                     /gene="moeB"
                     /locus_tag="AC159P_12150"
     CDS             7091..8578
                     /gene="moeB"
                     /locus_tag="AC159P_12150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014101324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HIR3696242.1"
                     /translation="MAVTVLIPTALRQYAGGSSELSVEAATAGEALERVTNEHAELRR
                     HLYNDQRALRNFVNVYVNDEDIRHTGGAATPVKDGDTLMIVPSIAGGATTEEEVEAAP
                     GVEHEAAPLPSLSNEEIARYSRHLIMPEVGVEGQRKLKAARVLMIGTGGLGAPLGMYL
                     AAAGVGTLGLVDFDVVEASNLQRQIVHGTKDVGRPKIESARDRLSDINPHIRIETHET
                     RLTSENALGLFRDFDIVVDGTDNFPTRYLVNDACVLTGKPNVYGSIFRFEGQASVFWA
                     ARGACYRCLYPEPPPPGLVPSCAEGGVLGVLPGIVGAIQANETIKLILGGGDALVNRL
                     LLFDAWKMRFREVKLRKDPACPVCGEHATIKQLIDYEEFCGLKPPPQSPDTQTPELEE
                     ITAAEFKQRLDRGDDLQIIDVREPGEYEIARIPGSKLIPLGEVAGRSNEIDETRETIV
                     HCKGGVRSAKAIDALKRAGFAGRLVNLKGGITAWSNDVDPTVPKY"
     gene            complement(8677..9687)
                     /locus_tag="AC159P_12155"
     CDS             complement(8677..9687)
                     /locus_tag="AC159P_12155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013581710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HIR3696243.1"
                     /translation="MSTLRRARTTDSTLLKNGQPMYYAIHHTTRFRYDRQISESVMEV
                     RMRPRDNGSQRCLRFDLGVSPRAEVFHYRDHLSNTVHHFDIPRPHTQLALTAHSVVEM
                     SPAEPLPEALGPGAWDELDRLAATGDHAFELLPSRFTEPTPLLRSLARELVCERRRGD
                     PLALLREINSAIHERFDYAPQTTHVDSPIDDALRARKGVCQDYAHIMLALLREHLGVP
                     CRYVSGYLFHRSEDQDRSASDATHAWVEALLPRLGWLGFDPTNNLLARERHIRVALGR
                     DYADVPPTRGVFKGEAATELKVAVKVEPCDAPPPEDLKLVMETVPPESIDQHQQQQQQ
                     QQ"
     gene            complement(9684..10649)
                     /locus_tag="AC159P_12160"
     CDS             complement(9684..10649)
                     /locus_tag="AC159P_12160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006563740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HIR3696244.1"
                     /translation="MLSRVADSLYWMSRYLERAEHTARLLDLNLHQMLDQSPEAAGPR
                     WERLLLSLRAPAIPGAAPPDAYRITDALTFDQSNGASIIACVSEARENARQVREQISS
                     EMWEQLNRLTLRVKGTGMEEIWHTEPHEFFQSVKDGAHLFLGITDATMSHGEGWQFIQ
                     LGRFIERAGATAALLDIYLPPHIEQQRGSAEQLDYLGLVGVLKSCTAFEAYCQVYTAH
                     VQPQSIAEFLLLNAECPRSVRFAVESLQAALQTISKLTGVRKAGRAERLAGRLRASLE
                     YGQVDEIIAGNLGAFLEDIQRRCNQLHAAIHQTYIAYPIDTALAS"
BASE COUNT         1786 a         3606 c         3642 g         1685 t
ORIGIN      
        1 cgcgctcgcg cccgtcgaga acacgctcgc cggctccgtc caccgctctt acgacctgct
       61 gctcgacagc tcgctccgca tcgccgcgga ggtcgtcatc cccatccgtc actttctcat
      121 cggctgcccc ggctccacgc tcgaacaggt cgagaccgtc gaatcgcacc cggtcgcgct
      181 cgcgcagtgc gagcgcttct tcgccgagca cgcgcacgtc cgccgcgtcg tcgccgaaga
      241 cacggcgggg agcgtcgccg cggtcgtcgg gcgcggcgac ccgacgcgcg ccgccatcgc
      301 gggcaagcgc gccgcggaca tctacggcgg ccaagtcctg cgcgaagggc tggaggatca
      361 ccgcgagaac tacacgcgct tcgtcctgct cacgccgtcg gacgaggtgg ccgcgggcgc
      421 ggacaagctc tcgctcgtcg tcaagctgcc gcaccggccc ggcgcgctgc accacgcgct
      481 cgaacccttc gcgcgccgcc gcatcgacct gctcaagatc gagagccgcc ccctgcgcgg
      541 ccagccctgg gagtaccact tctacctcga tttgaaagcc tcgcacgaag acgcggaggc
      601 cgccgccgcc ctcgccgagc ttcacgagag ggcggtcagc gtgcgccttc tgggttgtta
      661 cccttcggcc cgcgcgccgc tcgcctgaga tgaaatgaag aacacgtctc acagaattat
      721 ctttctgcgg ctctgccgcc tgatgtgcgg tgaggctgtg cctcaccgtg aagttcgctc
      781 ccgcaaaggc tgaggctgcg cctcagcgcc ggggcgtagc ccctacttga aaacgcgtgc
      841 ttgccggaag ggcatagccc ttccgcacat cgggcggcgg agccgcgaag actcggactt
      901 tcgcaaaagg tttaatgagc gtgttgactg acaacggttg ggagagggtg acggtcgtcg
      961 gctgcgggct gattggcgcg tcgttcgcgc tggcgctccg gcgcgcgggt gcgtgcgcgc
     1021 gggtcgcggg ctgggacgcc tcgccgtccg tcctcgacga agcgttggcg cgcggcatca
     1081 tcgacgaggt cgattacgcc atcgcggacg gcggcgtctc cgcgtcagac ctcgtctacc
     1141 tggcgacgcc cgtcggcgcg atcatcgagt tcctccgcga gcgcgcctcg cagatcaagc
     1201 cccgcgccgt cgttaccgac gcgggcagca cgaagcggga ggtgtgccgc gccgcgcgcg
     1261 aatcgctgcc gcgcgaccgc cgcttcgtcg gtgggcaccc cgtcgcgggg agccacctgc
     1321 gcggccccgc gcacgcgcgc gcagacctgt tcgaagcggc gccttacgtg ttgacgattg
     1381 acgagaacga acgtgaccgc gatgaagtcg gcggtcacgg gagcggcggc gacgagggcg
     1441 agcggcggac gcgcgaagcg ttcgcggaaa cgctgcgccg caccggcgcg cgcgtgacgc
     1501 tgatgacggc gcgcgagcac gaccgcgcga tggcgctcgt cagccatctg ccgcaactcg
     1561 tctcgagcac gctggcggcg acggtcgaga gccagccgga cggcgcggcg ctcggacggg
     1621 tcgcgggcgc gggcttcggc gacatgacgc ggctcgccgc gagcgagtgg gctgtgtggc
     1681 gcgacatcct tgagaccaac ccgtcggagg tcgctgccgc gctcgacact tttcttttga
     1741 agctcgcgat ggtgcgcgac gagttgcgcg actgctgcgc gggcgaccgc gacggactcc
     1801 gcgtgacaag ctctctgttc aaccactctc ggacgaccgg ctcgcggttc gagcaatccc
     1861 aaccgacaag cgcagtgaag ggctgaagtg aactcatgat tctcatcctg actccgaaca
     1921 tcgacacgga gggcgagacc taccggcgcc tgatggggca tctgtctcgg ctcccgaaca
     1981 tacaggcgcg cgtccaccgc gaacagggcg tggagcaatg cctgacggaa atctacctga
     2041 tcggcaacac cgccgccgtc tctctgaacg acatgaaaaa tctaccgggg gtggagcgcg
     2101 ccgtgcgcgt ctccgaagag taccgcgtca tcgggcgtca caagggagac gagcggccca
     2161 cgcacttcga gtaccagggc gtgcgcttcg ggcaggacac gctcaacgtc ttcgccggcc
     2221 tctgcgcggt ggacgaaccc cgacacgtcg aactgatgat gaaggcgctc cgcgacaacg
     2281 ggcaggtctg cacgcggatg ggcgcttaca agccgcgcac cagcccctac acctttcagg
     2341 gccacgggcg cgcgtgcctc acctacgtct tcgagctggc gggcaagtac ggcatcaagg
     2401 tcatcgcgat ggagatcacg cacgagtcgc acatcgccga gatccgcgac gcgctcgacc
     2461 tgacgggcag ccccaccggc gtcatgctcc agatcggcac gcgcaacacg cagaacttcg
     2521 agctgctgaa gatcgtcggg cggcagcacg agttccccgt cctgctgaag cgcggcttcg
     2581 gcatcacgct cgacgagtct ttgcacgccg ccgagtacct cgccagcgag ggcaaccgca
     2641 aggtcgtctt cggcctgcgc gggatgaaga cgagcatcgg cgacccgcac cgcaacttcg
     2701 tggacttcgc gcacgtcccg gtcgtcaagc gcctgacgcg gatgcccgtc tgcatcgacc
     2761 cctcgcactc ggtcggcacg cgcgcgcgct gcaccgaggg catcctcgac atcatgcacg
     2821 tcaccgcgca gggcgtcatc gccggggcca acatggtgct cgtcgatttc cacccggcgc
     2881 ccgaactcgc gctcgtggac gggccgcagg cgctcaggct cgaagagctg ccgctgttcc
     2941 tcgaagacgt ggccatcgcg cgcgaagcct acctgaagcg gctcgccctc gccgcgcgcg
     3001 accggcggac ggaggagctg gccgtggcct gatctccggg ccgagcggag cggtcggata
     3061 ggccactcaa gttttagaac taactgtcgt tgagtttacc gaaacattgg ttcaacgcag
     3121 agggcgcgga gcagacgcag agggcgcaga gaaaaccatt cttctgctct gcgccctctg
     3181 cgcgtcctcc gcgccctctg cgttgaaatg aatccgcgat aaactcaacc gcaaatcaag
     3241 tcctcgcgga ggttctgaca cttgttcaag cacgatctgg gagccggcgc cgagctgcgc
     3301 gtcctcgaag cgcggcacgc cgcggagttc cttcaattcg tggacgagaa ccgcgtcttc
     3361 ctcgggcgct ggctgcggtg gggcgaagag atcatgaccg tcgcggaggc cgaaaccttc
     3421 ctgcggcgcg gggtgaagac ttacgtcgat gatgggttgc cgtgggtggg catctggctc
     3481 gaaggcaaga tggtcggcgg cgtgctcttc ttccccgtcg agcatcgcat cgcggcgagc
     3541 gaggtcggct actggctggg cgagggcgcg acgggccgcg gcctcgtcac gcgggccgtc
     3601 gcggcgatgc tcgactacgc tttcggcacg ctcggcctgc accgcatggc gctgctggcc
     3661 gaggtcggca acgagaaatc gctcgcggtc gcgcggcggc tcggcttcac cgaagaaggg
     3721 ctgaaacgcg cgggcggcgt gaaccgcggc aagtacgtag acctcgtcgc cttctcgctg
     3781 ctggccgaag agtgggccgc gcgctcggcg cccacttctt gagaggggcg gcgcgggctt
     3841 catttatggc gacacaagtt ttatttatcg acggaaatcg tccggccttg actcgccccg
     3901 gacgcatcaa ctacattgag cacgtcatgc cgacagtaaa agcattcatc ggctgttgtt
     3961 gcccgcgcac atttccttag cgcggggcgt tcgattgtta ccgtggcgat ttcgggtgac
     4021 ttgctccacg ttaaaaacag ctaaggaacc gcatgggctt tgccgctccc gcgtttcctt
     4081 agcgcggggg tttttctttt tgggggaaag gggaagaggg aaaagggtga agaccaaaga
     4141 accttttcgc cttttcgcct cttcccctct tcgcctcagt tcttcgagcg gggcttgccc
     4201 ttcggccccc agtagtaggc gaagatgatg acgacgagcg cggcgatgat gaagaggcag
     4261 gtcaggagca gcaggacgag gtacttcatg acgcggatga cgttagcacg gtgaaggaag
     4321 atgagtcgag ttaaggacgg ccacgacatc acgacgcagc tcgtacacgc gggcgagcgg
     4381 ctgccgccgc ccgtgggcct gcccgtctcg acacccgtct acgcgacggc gaccttcacc
     4441 tacgactcga tggccgaggt cgatcaggtc ttctccggcg agaagcaggg ctacgtctac
     4501 acgcgctacg gcaacccgac gaccgccgcg ctcgaagagg cgatgcgcgt cctggaaggc
     4561 ggcgccgccg cctgcgccta cgccagcggc atggccgcgc tccacgccgc gctcttcgcc
     4621 tgcgagttgg cgccgggcgc caccgtcctc gcctcgcaag acctctacgg cgcgaccacg
     4681 gacctgctct acaacatctt cggctcgttc ggcgtgaaca ccgtcacggc tgacttcacg
     4741 aacacggaag ccctgcgcga gaaggcgcgc gaggtgcgcc cgcgcgtcct cgtcgcggag
     4801 accatctcga acccgctcct caaggtctgc gacatcgaag cctgcgccga ggtcgcgcgc
     4861 gaagcgggcg cgcgcttcgt cgtcgataac acgttcgcct cgccctacct gtgccagccg
     4921 ctccggcacg gcgcggactt cgctgtccac agcgcgacga agtacctcgg cggccacgcc
     4981 gacgtgatgg gcggtctcgt cgtcgcgcgc gacgaggtag accgcgcggc gctggtcggc
     5041 gtgatgaaac tggtcggcgg cgtgctcggc gtctgggagg cgcacgaagt catgcgcggt
     5101 ctgaagacgc tcgccgtccg gctcgaccgc cagtgcgaca acgcgcaccg cctcgccgaa
     5161 cggctcgacg gccacccgcg cctcgcgcgc gtccactacc ccgcgctcga cccttcgacc
     5221 gagacgcgcg aggttctggg ccgcgtcctg cgcgcgccgc acgcgggcgc gctcgtttcc
     5281 atcgaactcg cggaggacac cttcgacgcc gccttccgct tcatggacgc gctcgacctg
     5341 tgcgtccgcg cgacgagcct cggcgacgtg ttcacgggcg tcctgcaccc ggccaccgcc
     5401 tcgcaccgcg aggtcgcgcc cgcgcgccgc aagcgactcg gcatcgccga cggcctcgtc
     5461 cgcatctccg tcggcatcga agacatcggc gacatcatcg ccgacatcga gcaagcgttg
     5521 aaggccgtga accgtgaacc gtgacaagag aaaggcggtt tttactcgtc acggttgacg
     5581 gttcacggtt cacggcggtt gagttatgac cgaagcactt cttgaagagc cgttggtggt
     5641 cgcgccggag cgcggcggtc tgttgtcgct ggtcgggggc acgccgctgc tgcgtctgcg
     5701 gcgcgtcgcg cgcgacgtgc cggcgggcgt cgaggtctgg gcaaaggcgg agcacctgaa
     5761 ccccggcggc tcggtgaagg atcgcgcggc attggcgatg gtgctcgaag gcgagcgcga
     5821 ggggcggctc acgccgggca agaccatcct cgacgcgacg agcggcaaca cgggcatcgc
     5881 ctacgcgatg atcggcgcgg cgcgcggcta cgcggtcgcg ctctgcctgc cgcgcaacgc
     5941 gggcgcgggc cgcaagcgca tcctgcgcgc ctacggcgca gagcttgtcg agaccgaccc
     6001 gacgcgctcg accgacggcg cgcagctcgt cgcgcgcgag atggccgccc gcgcccccga
     6061 caagtatttc taccccgacc agtacaacaa ccccgccaac tggcgcgcgc actacgaaag
     6121 caccgcgccg gagattcggg agcagacggg cgcgcgcgtc acgcacttcg tcgccggact
     6181 cggcacgacg ggcaccttcg tcggcacgtc gcggcgcttg aaagagtacg acccgcgtgt
     6241 gcgcgccgtc gccgtccagc ccgactcgcc tttgcacgga ctcgaaggct tgaagcacct
     6301 cgcgacggcc atcgtgcccg gcatctacga cgcgtcgctc gccgacgagc acgcgaccgt
     6361 ctcgaccgaa gacgcgcagg agatgcgccg ccgcctcgcg cacgaagagg gcttgctcgt
     6421 cggcacgagc agcggcgcca acgtcttcgc cgcgctgcgc ctcgcgcgca cgctccccgg
     6481 cggctccgtg gtcgtcaccg tcctctgcga cggcggcgag cgctacctcg gcgaagagtt
     6541 ctgaagcgaa gagttcggaa agagggacaa cacggatgct ttcattgaca caggcgcacg
     6601 aggcggagat gcgcggccac ggcgagcgcg actatccgta cgagtgctgc ggcctgctcg
     6661 tcggtcgctt cgcggagggc ggcggcaagg aggtcgtcga gacctacccc atctcgaacg
     6721 cgcgcgagga ggaggccaag cgcaaccgct tcctcatccg ccccgaagag ttgctgcgcg
     6781 gcgaacgcca tgcccgcgcg cgcggcctcg acgtggtcgg cttctaccac tcgcaccccg
     6841 acgacgaggc catcccctcg cgctacgacc tcgaacacgc gtggcccacc tattcctaca
     6901 tcgtcgtctc cgtccgcgag gggcgcgccg tcgccctccg ctcgtgggag atggaggctg
     6961 atcgctcgaa gttcaacgaa gaagagataa gagccgtgaa ccgtgaaccg tcaaccgtga
     7021 aaagttaaaa ccggatttct cttgtcacgg tttacggttt acggcttacg tctttcgaaa
     7081 ggaaagaacg atggcagtca cagtcttgat cccgacggcg ctcaggcagt acgcgggcgg
     7141 aagctcggag ctgagcgtcg aggccgcgac ggcgggcgag gcgctcgaac gggtgacgaa
     7201 cgagcacgcg gagctgcgcc gccacctcta caacgaccag cgcgcgctgc gcaacttcgt
     7261 caacgtctac gtcaacgacg aggacatccg ccatacgggc ggcgccgcga cgcccgtcaa
     7321 ggacggcgac accttgatga tcgtcccttc catcgcgggc ggcgcgacga ccgaggaaga
     7381 ggtcgaggcc gcgccgggtg tcgaacacga agcggcgcct ttgccctcgc tctcgaacga
     7441 agagatcgcg cgctacagcc gccacctcat catgcccgag gtcggcgtcg agggtcagcg
     7501 caagttgaaa gccgcgcgcg tgctgatgat cggcaccggg ggcctgggcg ccccgctcgg
     7561 catgtatctg gcggcggcgg gcgtcggcac gctcggcctc gtcgatttcg acgtggtgga
     7621 ggcgtcgaac ttgcagcgcc agatcgtcca cggcacgaag gacgtgggcc gccccaagat
     7681 cgagtcggcg cgcgaccgcc tctccgacat caacccgcac atcaggatcg agacgcacga
     7741 gacgcgtctg acgagcgaga acgcgctcgg gctcttccgc gacttcgaca tcgtcgtgga
     7801 cggcacggac aatttcccca cgcgctacct cgtcaacgac gcgtgcgtct tgaccggcaa
     7861 gcccaacgtc tacggttcca tcttccgctt cgagggtcag gccagcgtct tctgggccgc
     7921 gcgcggcgcc tgctaccgct gcctctaccc agagcctccg ccgcccggcc tcgtgccttc
     7981 gtgcgcggag ggcggcgtgt tgggcgtcct gcccggcatc gtcggcgcga ttcaggcgaa
     8041 cgagaccatc aagctcatcc tcggcggcgg cgacgcgctc gtcaaccgcc tcctcctctt
     8101 cgacgcctgg aagatgcgct tccgcgaagt gaagctgcgc aaagacccgg cctgccccgt
     8161 ctgcggcgag cacgcgacca tcaagcagct catcgactac gaggagttct gcggcctcaa
     8221 gcccccgccg caaagcccgg acacgcagac gcccgaactc gaagagatca ccgccgccga
     8281 gttcaaacag cgcctcgacc gcggcgacga ccttcagata atcgacgtgc gcgaacccgg
     8341 cgaatacgaa atcgcgcgca tccccggctc gaagctcatc ccgctcggcg aagtcgcggg
     8401 ccgctcgaac gagattgacg aaacgcgcga gaccatcgtc cactgcaagg gcggcgtccg
     8461 cagcgccaaa gccatcgacg ccctcaaacg cgcgggcttc gccgggcgcc tcgtcaacct
     8521 caaaggcggc atcaccgcct ggtcgaacga cgtagaccca accgttccca agtactgacg
     8581 aagtgcgaca gaccggaagc cgtcaacgct gcatgctcaa cacgcgtgcg gcgctggcgg
     8641 cttccgcacg ttgaactgaa aggagcgacc agccgctcat tgctgctgtt gttgttgctg
     8701 ctgatgctgg tcgattgatt caggcgggac ggtttccatg acgagcttga ggtcttcggg
     8761 gggcggggcg tcgcagggtt cgaccttgac ggcgactttg agttcggtgg cggcctcgcc
     8821 cttgaagacg ccgcgggtgg ggggcacgtc cgcgtagtcg cgcccgaggg cgacgcggat
     8881 gtggcgctcg cgggcgagaa ggttgttggt ggggtcgaag ccgagccagc cgaggcgcgg
     8941 gagcagcgcc tcgacccatg cgtgcgtggc gtccgaggcc gagcggtctt ggtcttcgga
     9001 gcggtggaag aggtagccgc tgacgtagcg gcaggggacg ccgaggtgtt cgcgcaagag
     9061 cgcgagcatg atgtgcgcgt agtcctggca gacgcccttg cgcgcgcgga gcgcgtcgtc
     9121 gatgggcgaa tcgacgtggg tcgtctgcgg ggcgtagtcg aagcgctcgt ggatggcgga
     9181 gttgatctcg cggagcagcg cgagcgggtc gccgcgtcgc cgctcgcaaa cgagttcgcg
     9241 cgcgagcgag cgcagcagcg gggtcggctc ggtgaagcgg ctgggcaaca gctcgaaggc
     9301 gtggtcgccg gtcgcggcga gtcgatccag ctcgtcccac gcgccggggc cgagcgcctc
     9361 ggggagaggc tccgcgggcg acatctcgac gacggagtgg gcggtgaggg cgagctgcgt
     9421 gtgcgggcgc gggatgtcga agtggtggac ggtgttcgag aggtggtcgc ggtagtggaa
     9481 gacctctgcg cgcgggctga cgccgaggtc gaagcgcagg cagcgctggg agccgttatc
     9541 gcgcgggcgc atgcggactt ccatgacgct ctcgctgatc tgccggtcgt agcggaagcg
     9601 ggtcgtgtgg tggatggcgt agtacatcgg ctgtccgttc ttgagaagcg tgctgtcggt
     9661 cgtccgtgct cgtcgaagtg tgctcacgaa gcgagcgcgg tgtcaatcgg gtacgcgatg
     9721 taggtctggt ggatggccgc gtggagctgg ttgcagcggc gctggatgtc ttcgaggaaa
     9781 gcgccgaggt tgccggcgat gatctcgtcc acctgcccgt attcgagcga ggcgcgcagg
     9841 cgcccggcca gccgctcggc gcgtcccgcc ttgcgcacgc ccgtcagctt cgaaatggtt
     9901 tgcagcgcgg cctgcaagct ctcgaccgcg aagcgcaccg agcgcgggca ctcggcgttg
     9961 aggagcagaa actccgcgat ggactgcggc tggacgtgcg ccgtgtagac ctgacagtaa
    10021 gcctcgaagg cggtgcagga cttcagcacg ccgacgaggc cgagatagtc gagctgctcc
    10081 gcagagccgc gctgctgttc gatgtgcggc ggcaggtaga tgtcgaggag cgcggcggtc
    10141 gcgcccgcgc gctcgatgaa gcgcccgagc tggatgaact gccagccctc gccgtggctc
    10201 atcgtcgcgt cggtgatgcc gaggaagagg tgcgcgccgt ccttcaccga ctggaagaac
    10261 tcgtgcggct cggtgtgcca gatttcctcc atgccggttc ccttcacccg cagcgtcaga
    10321 cggttcaact gctcccacat ctccgagctg atctgctcgc gcacctgccg cgcgttctcg
    10381 cgcgcctccg agacgcacgc gatgatcgac gcgccgttcg actggtcgaa cgtcagcgcg
    10441 tcggtgatcc ggtacgcgtc gggcggcgcc gcgccgggga tcgcgggcgc gcgcaaagag
    10501 agcagcagcc gctcccagcg cggccccgcc gcctccggcg actggtcgag catctggtgc
    10561 agattcaggt cgagcagccg cgccgtgtgc tccgcgcgct caaggtagcg gctcatccag
    10621 tagaggctgt cggcgactct cgaaagcata aaagcagaag tcaggagtca gaagtcagaa
    10681 gccagaagga agacagcttt ttcattctgt cttctgact
//
LOCUS       DBNGOH010000623         2980 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_5040793, whole genome shotgun sequence.
ACCESSION   DBNGOH010000623 DBNGOH010000000
VERSION     DBNGOH010000623.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2980)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2980)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2980
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_5040793"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1624)
                     /locus_tag="AC159P_12165"
     CDS             complement(<1..1624)
                     /locus_tag="AC159P_12165"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HIR3696245.1"
                     /translation="MTRNPGKHKPDGRADAEAQGQQTPARAAGARAPEELYQEIFEQS
                     LGLISTHDLEGRLLTVNPAIAAQLGYAPAEMVGHNLRDFLAPSVRELFDDYLRRSRDQ
                     PAHSGVMRLLSKRGEERVWAYHNVRREEPGRAPYVLAHAHDITERKRAEDELRRTKEE
                     LEGRVAERTAELSEAMRRLELELAERRRAEEALRASEERYRELFENANDIVYTHDLEG
                     NYTSINKAGERVIGYARDEVLRMKMTDVLAPEYVDAARRMLARKLDGGAPTFYEVEIV
                     ARDGHRVPLEISSRLIHKDGRPVGVQGIARNLSSRRRAEEALRASEERYRELFENAND
                     ILYTHDLEGNFTAVNKAGERVTGYARRELLRMNIAHLVTSEHLELTRRMMVETLSGRG
                     RPVYEVEILARDGRKVILEVGIRVIREGRRPVAVQGTARDVTEAKTAREALRESEERY
                     ALAALGANDGLWDWDLRNHRVYFSPRWKAMLGHAEGEIETRPEEWFERIHPEDVESFR
                     RHLQEHVEGLTDHFECEHRMRHKTGTWRWMLAR"
     gene            complement(1993..2691)
                     /gene="purQ"
                     /locus_tag="AC159P_12170"
     CDS             complement(1993..2691)
                     /gene="purQ"
                     /locus_tag="AC159P_12170"
                     /EC_number="3.5.1.2"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020713915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HIR3696246.1"
                     /translation="MKFGVIVFPGSNCDHDAYHVISKHVGQPVDFIWHRDTDLSTYDA
                     VIIPGGFSYGDYLRAGALARFSPVMSSVQEFAARGGFVLGVCNGFQILCEAGLLPGAL
                     IRNRDLHFICQHVNVRVETTETPFTHGLKAGDVLSLPVAHGEGNYVCDDATLEELKRE
                     DRIVFRYADAAGQLTDEANVNGSREAIAGNCTRDRNVLGMMPHPERACEDLLGSADGR
                     DIFRSLAATLAAAK"
     gene            complement(2688..>2980)
                     /gene="purS"
                     /locus_tag="AC159P_12175"
     CDS             complement(2688..>2980)
                     /gene="purS"
                     /locus_tag="AC159P_12175"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HIR3696247.1"
                     /translation="AAIGAAAIAREDRKSMKARVFVTLKPGVLDPQGKAIHHSVELIG
                     FAAVRDVRQGKYFEIALDDSLDETRARAEAERMAKDVLSNPVIEDYRVEIER"
BASE COUNT          436 a          956 c         1058 g          530 t
ORIGIN      
        1 cgcgcgcgag catccagcgc caggtgccgg tcttgtggcg catgcggtgc tcgcactcga
       61 agtggtcggt gagaccttcg acgtgctcct gaaggtggcg gcggaagctt tccacgtctt
      121 cggggtggat gcgctcgaac cactcctcgg ggcgggtctc gatctcgcct tcggcgtggc
      181 cgagcatcgc cttccagcgc ggcgagaagt agacgcggtg gttgcgtaag tcccagtccc
      241 acagcccgtc gttggcgccg agcgcggcga gcgcgtagcg ctcttccgat tcgcgcaggg
      301 cctcgcgcgc cgtcttggcc tcggtcacgt cgcgcgcggt gccctgcacg gcgacggggc
      361 ggcggccctc gcggatgacg cggatgccga cctcgaggat gactttccga ccgtcgcgcg
      421 cgaggatttc gacctcgtag acggggcgcc cgcggcccga gagcgtctcg accatcatcc
      481 ggcgcgtcag ctcaaggtgc tcggacgtga cgaggtgcgc gatgttcatc cgcagcagct
      541 cgcggcgcgc gtagccggtg acgcgctcgc ccgccttgtt gacggcggtg aagttgcctt
      601 cgaggtcgtg cgtgtagagg atgtcgttgg cgttctcgaa cagctcgcgg tagcgctcct
      661 cgctcgcgcg cagcgcctct tcggcgcggc ggcgcgagga gaggttgcgg gcgatgccct
      721 gcacgccgac ggggcgcccg tccttgtgga tgaggcgcga gctgatttcg agcgggacgc
      781 ggtggccgtc gcgggcgacg atctcgacct cgtagaaggt cggggcgccg ccgtcgagct
      841 tgcgcgcgag catacggcgc gcagcgtcca cgtactcggg cgcgagcacg tcggtcatct
      901 tcatgcggag cacttcgtcg cgcgcgtagc cgatgacgcg ctcgcccgcc ttgttgatcg
      961 aagtgtagtt gccttcgagg tcgtgcgtat aaacgatgtc gttggcgttc tcgaacagct
     1021 cgcggtagcg ctcctcgctc gcgcgcaggg cctcttcggc gcggcgccgc tcggcgagtt
     1081 cgagttcgag gcggcgcatc gcctcgctca gctccgccgt gcgctcggcg acgcggccct
     1141 ccagctcctc cttggtgcgg cgcagctcgt cctcggcgcg cttgcgctcg gtgatgtcgt
     1201 gcgcgtgcgc gaggacgtaa ggggcgcggc ccggctcttc gcgccggacg ttgtggtagg
     1261 cccagacgcg ctcctcgccg cgcttcgaga ggaggcgcat gacgccgctg tgcgcgggct
     1321 ggtcgcgcga gcggcggagg tagtcgtcga agagttcgcg gaccgagggc gcgaggaagt
     1381 cgcgcaggtt gtggccgacc atctcggcgg gcgcgtagcc gagctgcgcg gcgatggccg
     1441 ggttgacggt cagcaggcgg ccttcgaggt cgtgggtgct gatgaggccg aggctctgct
     1501 cgaaaatttc ctgatagagt tcttcgggcg cgcgcgcgcc cgcggcgcgt gcgggcgtct
     1561 gctgcccctg cgcctcggcg tccgcacgcc cgtccggctt gtgcttacct ggattcctgg
     1621 tcatgcgcgc ctccgtgtcg ggaggagacg agaagccgcc ggctacgaac atgaggcgta
     1681 cgtaggcggc ggagacttcg gccagattcc atagtttagg cagaatgacg ggaaccgggt
     1741 cgtggcctgt aatcgaaggg atgaatgccg gggcgcgctc cccgctcgat atggcccgct
     1801 gagctttttt taccacatgc acgggccacc cgcaacgaca atttaacaaa aggcgaaaag
     1861 gttaaaaggg agaaaggggg aaaaggttaa aaggggaaaa ggcgaaaggg gaagaagcga
     1921 aaggggaaga agcagcgcac gttgcattct ctcccttttc cccctttccc cttttaacct
     1981 tttcccccct tcttatttcg cggcggcgag cgtggcggcg agcgagcgga agatgtcgcg
     2041 cccgtccgcg gagccgagca ggtcttcgca ggcgcgctcc gggtgcggca tcatgccgag
     2101 cacgttgcgg tcgcgcgtgc agttgccggc gatggcttcg cgcgagccgt tgacgttggc
     2161 ctcgtccgtg agctggccgg cggcgtccgc gtagcggaag acgatgcggt cttcgcgctt
     2221 cagctcttcg agcgtcgcgt cgtcgcacac gtagttgccc tcgccgtgcg ccacggggag
     2281 cgacagcacg tcgcccgcct tcagcccgtg cgtgaaaggc gtctcggtcg tctcgacgcg
     2341 cacgttcacg tgctggcaga tgaagtgcag gtcgcggttg cggatcagcg cgcccggcag
     2401 caagcccgcc tcgcacagaa tctggaaccc gttgcacacg ccgagcacga acccgccgcg
     2461 cgcggcgaac tcctgcaccg aactcatcac cggcgagaag cgcgcgagcg cccctgcgcg
     2521 caggtagtcg ccgtacgaga agccgccggg gatgatcacc gcgtcgtacg tcgaaagatc
     2581 cgtatcgcgg tgccagatga agtccacggg ctgccccacg tgcttcgaga tgacgtggta
     2641 cgcgtcgtgg tcgcagttcg agccggggaa cacaatcacg ccgaatttca tctttcgatc
     2701 tccacgcggt agtcctcgat gaccgggttg gacaaaacgt ccttcgccat gcgctcggcc
     2761 tcggcgcggg cgcgcgtctc gtcgagcgag tcgtcgagcg cgatctcgaa gtacttgcct
     2821 tgccgcacgt cgcgcaccgc ggcgaagccg atcagctcga cggaatggtg gatggccttg
     2881 ccctgcgggt cgagcacgcc gggcttcagg gtgacgaaaa cgcgcgcctt catgcttttc
     2941 ctgtcctcgc gggcaattgc tgccgcccct atggcggccc
//
LOCUS       DBNGOH010000624         5668 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4857545, whole genome shotgun sequence.
ACCESSION   DBNGOH010000624 DBNGOH010000000
VERSION     DBNGOH010000624.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5668)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5668)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5668
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4857545"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1427)
                     /locus_tag="AC159P_12180"
     CDS             complement(<1..1427)
                     /locus_tag="AC159P_12180"
                     /inference="COORDINATES: protein motif:HMM:NF016995.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis ResB family protein"
                     /protein_id="HIR3696248.1"
                     /translation="MSAIRDARGATLNTNEVKAATPSSTSERVMQSLSSVRLGVALLV
                     LLAAACMTGMLIMQANMEGFDKYYAGLTPSQQLLYGKLGFFDIYHTWYFNALLLVLSL
                     NIVLSSVDNFPKAWAFISRPKLDASARWLGGQEQHAALKVDGDDPQATAARFSATCRS
                     LGLKTKVTESGGKTFVFAERGAWNRLGAYAVHVALLVIFAGGFLTAQFGHTGQVAFGP
                     GESASEMNETAFGLEGPRQITVALPFEIECTDVQQKLIDKSGPTSPVNTLDWLTRIRI
                     KDPERGETEALVHMNAPFDYRGYRFFQSSFVPDGKARTIRLRVTPEQPGAQSQELDIM
                     RDSSAQLSDGTVVKFAGFFSDFVLNGTRADSKSPEYNNPAAAIQVVKVSGEADKGYAL
                     QPSSAKAGPMVGRAVAGYKLELIDFEKVGAAHVLSVQKDPGAGVVYVGFSLLILTLAA
                     VFLFSHQRLWALVEPCGEGRYEITLG"
     gene            2182..3246
                     /locus_tag="AC159P_12185"
     CDS             2182..3246
                     /locus_tag="AC159P_12185"
                     /inference="COORDINATES: protein motif:HMM:TIGR03508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DmsE family decaheme c-type cytochrome"
                     /protein_id="HIR3696249.1"
                     /translation="MKRLKTIKWLLVAAWVLGVVLLLGGTSFSNPTTASTQKKQPTPK
                     STAAQPGDYVGEDACTDCHTDQTNHYSQTAHAKLKKDSSWKGKVVGCESCHGAGRAHV
                     ETMAEAIGNGVEPSAVKDKKIVYLAGLPPKESSDTCLACHAGREEHNNYRRGEHWRND
                     VGCIDCHSAHTPTTGANKAGSNVFVSSANAEKPGFAAEKMLKASEPQLCMQCHTETKH
                     QFNKPFKHKVLEGAMKCSDCHNAHGGFESKQARLATGADAACIKCHTDKQGPFTYEHA
                     PVKTEGCASCHTPHGSANPRLLRTSSVAQLCLECHSQDHGVGAQEPAGPSHNLAAQYR
                     DCTACHVKVHGSHTSPVFMR"
     gene            3309..5585
                     /locus_tag="AC159P_12190"
     CDS             3309..5585
                     /locus_tag="AC159P_12190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696250.1"
                     /translation="MKSEVTRMLRVLPGLALCLALAAAQARAQQPSPSPQKSADAQTQ
                     STAPSKKAEDAAPYTITSSVEIGYRGLSVDGNLDKYQSDLNYKAGPRLFDSSLLMQAK
                     PGEGGPFDSLLVTTTGWGADPNGHMRISAEKSVWYRFDGSYRRFKYFRSLNNIANPNY
                     RTASCPTCRTTEAGGGQHGYDTRQGVGDFDLVILPKNERVRFNVGFSPSRYSGPAFTT
                     WHFGGDDFMLLSQTRYRSNDFRVGADWKLGPVDFSFSQGFRRFRDDTVIDENTVNLGV
                     NPTPSNAFLSSLSREQPVRGSVNYSRFSAHTLLAKRLDITGRFIYSSAKTNFDWTETV
                     TGTNFNTRITNIPGAINPPNILTLGVWDFAGSAKRPNTLGDFGVTYLVTDNLRLSNTF
                     RVETFQISGGTFYNGLFNLTRTNGTGAVSLRPAGSASELTKYRKFQNTIEGDYQFGAR
                     YSVRFGYRYGSRHIEHFFAGANLASNGAPPLAATSEEEDNHTNTIFGGVKARPVKGWT
                     LSFDAERGTSDNVFTRTGNYDYWNVRARSRYAVRRDLRFNVAFIARDNSNPSEIDHSG
                     TTVSLTDFGVDIKSRVFTSSVDWTANDKLSFSAGYNYHWQNSDAVIQYAFNSTCPPPL
                     PGSTNSCITGRSLYFVRNHYFNFDTVAQPFSRVTLYAAYRINKDLGQGDRISDPLNNR
                     LVTSYPMSYQSPEARIAVRLNRWLDWNLGYQYYNYNESRLADYNFGTFIVSPRPQNYH
                     AHLPYTSLRIYFGRNGER"
BASE COUNT         1106 a         1936 c         1712 g          914 t
ORIGIN      
        1 cccaaagtga tttcgtagcg cccttccccg cacggctcga cgagcgccca caggcgctgg
       61 tgcgagaaga ggaagacggc ggcgagcgtc aggataagca aggagaagcc gacgtagacg
      121 acgccggcgc cggggtcttt ctgcaccgac aggacgtgcg ccgcgccgac cttctcgaag
      181 tcgatcaact cgagcttgta cccggccacc gcgcgcccga ccatcggccc tgcctttgcc
      241 gatgaaggct gcaaggcgta gcccttgtcg gcctcgcccg aaactttgac gacctgtatg
      301 gcggcggcgg ggttgttgta ctcgggcgac ttcgagtcgg cacgcgtgcc gttcaaaacg
      361 aagtcggaga agaagcccgc gaacttgacg accgtgccgt ccgagagctg cgccgaagag
      421 tcgcgcatga tgtcaagctc ttgactctgc gcgcccggct gttcgggcgt gacgcgcagg
      481 cgtatcgtgc gcgccttgcc gtcggggacg aagctcgact ggaagaagcg gtagccgcga
      541 tagtcgaagg gcgcgttcat gtggacgagc gcctccgtct cgccgcgctc agggtctttg
      601 atacgaatcc gcgtgagcca gtcgagtgtg ttcacgggcg aggtcggccc cgacttgtcg
      661 atgagcttct gctgcacgtc ggtgcattcg atctcgaacg ggagcgcgac ggtgatctgc
      721 cgcgggcctt cgaggccgaa ggcggtctcg ttcatctccg aggcgctctc tccggggccg
      781 aaggccacct ggcccgtgtg accgaactgc gcggtgagaa acccgcccgc gaagatcacg
      841 agcagcgcga cgtggacggc gtaagcgccc agacggttcc acgccccgcg ctcggcgaag
      901 acgaacgtct tgccgccgct ctcggtgact tttgttttaa gaccgagaga gcggcacgtg
      961 gcgctgaagc gcgcggcggt tgcctgtgga tcgtcgccgt ctactttcag cgccgcgtgc
     1021 tgctcctgcc cgccgagcca gcgggcggag gcgtcgagct tggggcggga gatgaaggcc
     1081 cacgccttcg ggaagttatc caccgaggag aggacgatgt tgagcgagag gacgagcagg
     1141 agcgcgttga aataccatgt gtgatagatg tcgaagaagc cgagcttgcc gtagaggagc
     1201 tgctgcgaag gcgtcagccc ggcgtaatac ttgtcgaagc cctccatgtt ggcctgcatg
     1261 atgagcatcc ccgtcatgca ggccgcggcc agcagcacca gcagcgccac gccgaggcgc
     1321 accgagctga gcgactgcat cactcgctcg ctcgtgctcg acggcgtcgc ggccttgacc
     1381 tcgtttgtgt tcagtgtcgc gccgcgcgcg tcccttatcg ccgacatcgg ttttagcctt
     1441 tggatttatt caccggactg tggttgatat ttatcccgcc ctcgacacga gcgaccatgg
     1501 acggacgccg cccgaaacgg caactgatgt gccagcaccg agatgccgtc ggcgtgtccg
     1561 tctcgccgtg tgttatctcg cggcttacat cctcccgcct tccgctcgcc cttagttgcg
     1621 gcccgtcttc ggcggtaaaa ttcccacagt catgggtaat tatccgcatc cgtaatgcgg
     1681 cccaatcatc gcccttgccc gccctcgccc acgcaatcag tctcagcctc gcgcttcgta
     1741 aaaacgtgaa tccccctcgc cgtgcgacgg attctttgac cgcaacctcg atccgcccgc
     1801 cgcgctaacg tcgaatttcc atcgcgcgga acgggcgttg ctcttgacgc ctacagagag
     1861 accggcgtga ggccgctcgc ggcgacagcc tcgccagaaa ccaagccgcc ggattctccg
     1921 aagaaacagg ccggacggcg gttcctttcg ccattcgcac acgaccccgg ccttcaagca
     1981 ttcgctcttg accgcagtcc ccctcgccga ggaggcgttg atggtgggcg aacgactacg
     2041 aacccttatt cccgtgttgg ccggcacggc gcgtgtcggc tgtcgcggct ttccgtgttg
     2101 ttaggaatga tccgccggag cgcccgccgc gtcggaccga ccgaggctcc acgcagcagg
     2161 ccgaaagttt ggaggcaaga gatgaaaagg ctgaagacca tcaagtggtt gctggtcgcg
     2221 gcgtgggtct tgggcgtcgt gctgctcctc ggcggcacgt cgttctccaa cccgacgacc
     2281 gcttccacac aaaagaagca gccgacgccg aagagcaccg ccgcgcagcc cggcgactac
     2341 gtcggcgaag acgcgtgcac ggactgtcac accgaccaga ccaatcacta ctcccagacg
     2401 gcccacgcga agctcaagaa agattcgagc tggaagggca aggtcgtggg ctgcgagtcg
     2461 tgccacggcg cgggccgcgc gcacgtcgag acgatggccg aggccatcgg caacggggtc
     2521 gagccttccg ccgtgaagga caagaagatt gtttacctcg cggggctacc gccgaaagaa
     2581 tcttccgaca cctgcctcgc gtgccacgcc gggcgcgagg agcacaacaa ctaccgccgc
     2641 ggcgagcact ggcgcaacga cgtcggctgc atcgactgcc actcggcgca caccccgacg
     2701 acgggcgcga acaaggccgg ctcgaacgtc ttcgtctcgt cggccaacgc cgagaaaccc
     2761 ggcttcgccg ccgagaagat gttgaaggcg agcgagcctc agctctgcat gcagtgccac
     2821 accgagacca agcaccagtt caacaagcct ttcaagcaca aggtgctcga aggcgcgatg
     2881 aagtgctcgg actgccacaa cgcgcacggc gggttcgagt cgaaacaggc gcggctggcg
     2941 acgggggcgg acgccgcctg catcaagtgc cacacggaca agcaggggcc gttcacctac
     3001 gagcacgcac ccgtcaagac cgagggctgc gcctcgtgcc acacgccgca cggctcggcg
     3061 aacccgcggc tcttgaggac gagcagcgtc gcgcagctct gcctcgaatg ccacagccag
     3121 gatcacggcg tcggcgcgca ggagcctgcc ggcccttcgc ataacctcgc cgcgcagtac
     3181 agagactgca cggcctgtca cgtcaaggtt cacggctcgc acaccagccc cgtcttcatg
     3241 agatgaggcg gttgcattga gaaaggcatt gaaggctcac acgaatgttg agcgagagga
     3301 cggacgaaat gaaaagtgaa gtcacacgaa tgctgcgcgt gctgcccggt ctggcgctgt
     3361 gtctggcgct ggcggcggct caggcacggg cgcagcagcc atcgccaagc ccacagaagt
     3421 ccgccgatgc tcagactcaa agcaccgccc cctccaagaa ggcggaggac gccgcgccct
     3481 acacgatcac ttccagtgtg gagatcggct accgcggtct gagcgttgac ggcaatctcg
     3541 acaagtacca gagcgacctc aactacaagg ccggcccgcg ccttttcgac tcaagcctgc
     3601 tgatgcaggc gaagccgggc gagggcggcc ccttcgacag tctgctcgtc accactacgg
     3661 ggtggggcgc cgaccccaac ggccacatgc ggatcagcgc ggagaagtcc gtgtggtatc
     3721 gcttcgacgg gagctaccgc cggttcaaat acttccgctc gctgaacaac atcgccaacc
     3781 ccaattaccg gacggccagt tgcccgacgt gccgcacgac cgaggccggc ggcgggcagc
     3841 acggctacga cacgcggcag ggggtcgggg atttcgacct cgtcatcctg ccgaagaacg
     3901 agcgcgtgag gttcaacgtc ggcttctcgc cttcgcgcta cagcggcccg gccttcacca
     3961 cctggcactt cgggggcgac gacttcatgc tgctttcgca gactcgttat cgctcgaacg
     4021 acttccgcgt cggagccgac tggaagctcg gccccgtgga cttctctttc tcgcagggct
     4081 tccgccgctt ccgcgacgac accgtcatcg acgagaacac agtcaacctc ggcgtcaacc
     4141 cgacgccgtc aaacgccttc ctgtcgagcc tgagccgcga acagcccgtg cgcgggagcg
     4201 tcaactacag ccgcttcagc gcgcacacgc tgctcgccaa acgtctcgac atcactggcc
     4261 gcttcatcta cagcagcgcg aagaccaact tcgactggac tgagaccgtc accggcacca
     4321 acttcaacac gcgcatcacg aacatccccg gcgccatcaa cccgcccaac atcctgacgc
     4381 tcggcgtgtg ggacttcgcg ggcagcgcca agcggcctaa caccctcggc gacttcggcg
     4441 tgacctacct cgtgacggat aatctgaggc tctcgaacac gttccgcgtc gagaccttcc
     4501 agatcagcgg cggcaccttc tacaacggct tgttcaacct cacccgcacc aacggcacgg
     4561 gcgcggtctc gctccgcccc gccggcagcg ccagcgagct gaccaagtac cgcaaatttc
     4621 agaacaccat cgagggcgat taccagttcg gcgcgcgcta ctccgtacgc ttcggttacc
     4681 gctacggaag ccggcacatc gagcacttct tcgcgggcgc caacctcgcc agcaacgggg
     4741 cgccgccgct cgccgccacg tccgaggagg aggacaatca caccaacacg atcttcggcg
     4801 gcgtcaaggc ccgccccgtc aaaggctgga cgctctcgtt cgacgccgag cgcggcacgt
     4861 ccgacaacgt cttcacgcgc accggcaact acgactactg gaacgtgcgc gcgcgcagcc
     4921 gctacgccgt gcgccgcgac ctgcgtttca acgtcgcctt catcgcgcgc gacaactcca
     4981 acccctccga aatagaccac agcgggacga ccgtctcgct cacggacttc ggcgtggaca
     5041 tcaagtcgag ggtcttcacc tcgtccgtgg actggacggc gaacgacaag ctctcgttca
     5101 gcgcgggcta caactaccac tggcagaaca gcgacgccgt catccagtac gccttcaaca
     5161 gcacctgccc gcctcccttg ccgggcagca cgaactcgtg catcacgggc cgcagcctgt
     5221 acttcgtcag gaaccactac ttcaacttcg acacggtcgc gcagcccttc tcgcgcgtga
     5281 cgctctacgc ggcctaccga atcaacaaag acctggggca gggcgaccgc atctcggacc
     5341 cgctgaacaa ccggctggtc acctcgtacc cgatgagcta ccagtcgccc gaagcgcgca
     5401 tcgccgtccg gctcaaccgc tggcttgact ggaacctcgg ctatcagtat tacaactaca
     5461 acgaaagccg actcgccgat tacaacttcg gcacgttcat cgtcagcccg cggccccaga
     5521 actaccacgc gcatctgccc tacacgtcgc tccgcatcta cttcggacgt aacggcgagc
     5581 gataaatcga gaccgggaag gacggggcag cccgatcgaa catggattgg acgaagcaga
     5641 tagaggcgac ggatgaagca ggtcaaca
//
LOCUS       DBNGOH010000625         1691 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_641532, whole genome shotgun sequence.
ACCESSION   DBNGOH010000625 DBNGOH010000000
VERSION     DBNGOH010000625.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1691)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1691)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1691
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_641532"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..233
                     /locus_tag="AC159P_12195"
     CDS             <1..233
                     /locus_tag="AC159P_12195"
                     /inference="COORDINATES: protein motif:HMM:NF018647.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HIR3696251.1"
                     /translation="TSSSSKEVRVQLAGARGVGRGGKAFVLSSNDLKAENSLDEPTKV
                     APVERPLSVSSGDFTHTFAPRSMTVLRVGVTR"
     gene            402..>1691
                     /locus_tag="AC159P_12200"
     CDS             402..>1691
                     /locus_tag="AC159P_12200"
                     /inference="COORDINATES: protein motif:HMM:NF046388.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696252.1"
                     /translation="MKRKHNGFRPPALALRLACCVLLSCHMAFAQAARQASITVDARR
                     VENRLSPLLYGQFLEFMYEGIKGGVHAELIRNRGFEESANSIGLSRHWERYPDDRNDD
                     YALAFAQDEKHPYPPRRKTKIDTGEKETREHSLFVEAGNGVIPRHGLYQPRVPVSAGV
                     EYRGYLWLKSQDYAGGITLALEADADGGEVYAESSASVVAGDWRKYEFTLKPTKTDPL
                     ARLCVLFDGRGRAWVDQVSLMPSDAAPGGVRRDVFEKIKALKPAFLRWPGGNVAQDYR
                     WPWATGPRDERFTWANLSWKNEPEPGDFGTDEYIRFARAVGAVPTITVNVEGRGATAE
                     EAAAWVEYCNGPPSSKFGAMRAANGHPAPYDIKLWEIGNEIWGNWVRGHSDAETYARN
                     YNLYVRAMRAVDPTLRFIAVGDNDMQWNRTVLARAGAD"
BASE COUNT          293 a          573 c          552 g          273 t
ORIGIN      
        1 acacatcttc gtcttcgaag gaagtccgcg tccaattggc tggcgcgcgc ggcgtgggcc
       61 gtggcggcaa ggctttcgtc ctctcaagca acgacctgaa ggccgagaac agcctcgacg
      121 agccgacgaa ggtcgccccc gtcgagcgcc ctttgtcggt ctcctccggc gacttcacgc
      181 acacgttcgc cccgcgctcc atgacggtgc tgcgcgtcgg cgttacgaga tgagcgggca
      241 acattccgaa cgtttgtagg ggcagggctt gtcctcggcc cgcgcttccg cttgaggtca
      301 tcttgagtgg caaagaaagt ctgcgggcag ggacaagccc tgcccctaca atcatcgtgc
      361 ttgttcgtca ttctcctgat gctgtctctg tcgtgcagac aatgaaaagg aagcacaacg
      421 gctttcgtcc gcccgcgctc gccctgcggc tcgcgtgctg cgtgctgctc tcttgtcaca
      481 tggccttcgc gcaggccgcg cggcaggcga gcatcacggt tgacgcgcgc agggtcgaga
      541 accgcctcag cccgctgctc tacggccagt tcctcgaatt catgtacgag ggcatcaagg
      601 gcggggtgca cgcggaactt attcgcaacc gcggcttcga agaatcggcg aactccatcg
      661 gtctttcgcg ccactgggag cgctacccgg acgaccgcaa cgatgactac gcgctcgcct
      721 tcgcgcaaga cgaaaagcac ccttacccgc cgcgccgcaa gacgaaaatc gacaccggcg
      781 agaaggagac gcgcgagcac tcgctgttcg tcgaggcggg gaacggcgtc atcccgcgcc
      841 acggcctcta ccagccgcgc gtaccagtca gcgcgggcgt cgaataccgc ggctacctct
      901 ggctgaagtc gcaggattac gcgggcggca tcacgctcgc gctcgaagcc gacgccgacg
      961 gcggagaggt ctatgcggaa tcatccgcga gcgtcgtcgc gggcgactgg cgcaagtatg
     1021 agttcacttt gaagcctacg aagaccgacc cgcttgcacg gctctgcgtc ctcttcgacg
     1081 ggcgcggtcg cgcgtgggtc gatcaggtct cgctgatgcc gagcgacgcg gcgccgggcg
     1141 gcgtgcgccg cgatgtgttc gagaagatca aggcgttgaa gcccgccttc ctgcgctggc
     1201 cgggcggcaa cgtcgcgcag gactaccgct ggccgtgggc cacggggccg cgcgacgagc
     1261 gcttcacgtg ggcgaacctc tcttggaaga acgagcctga gccgggcgac ttcggcacgg
     1321 acgagtacat ccggttcgcg cgcgccgtcg gcgccgtgcc gaccatcacg gtcaacgtcg
     1381 aggggcgcgg cgccaccgcg gaagaggccg ccgcgtgggt cgaatactgc aacggcccgc
     1441 cctcttcgaa gttcggcgcg atgcgcgcgg cgaacggcca ccccgcgccc tacgacatca
     1501 agctctggga gatcggcaac gaaatctggg gcaactgggt gcgcggccac tcggacgccg
     1561 aaacctatgc gcgcaactac aacctttacg tccgcgcgat gcgcgccgtt gaccccacgc
     1621 tccggttcat cgccgtcggc gacaacgaca tgcagtggaa ccgcaccgtg ctcgcgcgcg
     1681 ccggggcgga c
//
LOCUS       DBNGOH010000626         4957 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4673771, whole genome shotgun sequence.
ACCESSION   DBNGOH010000626 DBNGOH010000000
VERSION     DBNGOH010000626.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4957)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4957)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4957
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4673771"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..607)
                     /locus_tag="AC159P_12205"
     CDS             complement(<1..607)
                     /locus_tag="AC159P_12205"
                     /inference="COORDINATES: protein motif:HMM:NF036516.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M61 family metallopeptidase"
                     /protein_id="HIR3696253.1"
                     /translation="MRHTRLYVALAAAFVLALFVSAPARAQDRTAPEISYTVSMSQPH
                     THLLEVEARLRWREGAAPPSRAELVMPVWTPGSYLVREFERHVQDFAAQDGAGRALQW
                     SKANKNTWRVETAGARDVRVRYSVYANELTVRTNELNDRHAFWNNSALLMYPEGHLAA
                     PSTVRVVPREGWKIATGLAAVSGERNTFRAESFDILYDSPFL"
     gene            824..1294
                     /gene="trxA"
                     /locus_tag="AC159P_12210"
     CDS             824..1294
                     /gene="trxA"
                     /locus_tag="AC159P_12210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HIR3696254.1"
                     /translation="MAIVTCEKCGAKNRVDEGAAAASQPVCGRCGAKLEAGAGGHGGG
                     ASGDGKPLTVTDESFAREVVEASAERPVLLDCWAPWCGPCRMIAPALDELAAESGGRY
                     RIAKLNVDENPRTSSTLGIRSIPALFVYKDGQIVDRIIGAQPKQVLAARLAAHV"
     gene            complement(1471..4194)
                     /locus_tag="AC159P_12215"
     CDS             complement(1471..4194)
                     /locus_tag="AC159P_12215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3696255.1"
                     /translation="MKRIQSSSLSIVLAAAVSVVACAAVVAAQPAQKPKPKPAAAKPK
                     SGAAAPKAKAAPADEEASKAELEEVVKLAPAERVARLEEFVKKNAESETRARAVEFLT
                     SARAALGDEKLRNGDRLAGAELFRAAVAGAPAAMPDKLFYEVVSQLPANLHVLGEQEA
                     ALELARRIEAKVKDNPQRLLSVAAFYLGIERATEAARVAEAAVALKPDLAAAHLALGA
                     AYRFSLRLDDAAREFARASELDPASHGARRSLADLLRATGKAEEALALYRERLAADAT
                     DTGARTGLVLSLFDAGKREEAERELQGALMEQSSNLPLLVGASYWYAAHDEGVLALEL
                     AERAVALEPRFRWVWSRVALSRALLARGRPLDAERALRPARELGRFPTLDYELASVLA
                     AAGLYEEAAEELARSFTIKGGQIETRLAGGIAARSASFTELLAPERRAGLFQFKAADT
                     ETNARVLKGLLALHQATKTGAAKEAEAAEAAAEFVAGEDPMRAFRELHAAKRLTQGGV
                     AHRVAVEATESAKRGLDAALGVPHASVALFAEDLYDVRARALASGMTTTAPDASRELM
                     SVVMRGRIEDLAGWALFVGGDAKEAAVRLRRAVSVLPENSIWWKTAQWHLGAALDASG
                     NQKEALAAYVRSYRLGAPDPVRLAVIEALYRRINGSLEGLERLLAAPAPTAVAANRTG
                     QPITAASTSTTGETATPPPGETTATDPSIVKTAAPEPTPTPAPTETPAPAETTPATDP
                     ATSPAPSPTPEPTPTPTEAPAPTPTPAPTTTEAPAPTETPTPTPEQTTPPAETTAATS
                     TTSRPAPVREAKGAGGGCALTVNEGSVSIPVGGSVTLVVRFVNHTGPRLPRLNVSTPN
                     WADITILAEPRSEADGDASRFTVSSTSKKTGAFNVAFASPCGKKQVTVNVQ"
     gene            complement(4309..>4957)
                     /locus_tag="AC159P_12220"
     CDS             complement(4309..>4957)
                     /locus_tag="AC159P_12220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HIR3696256.1"
                     /translation="VERRVGLRHTLVAFTADHGVAPVPEHAAAMKLSGGRVKPSDVTS
                     AVRGALRARFGDEAEKYLLAFDNNNLYFDHQALRRDRIGHEEVERLAGEAALAVPGIA
                     RFFTRTQLAARGVSPADPVARRVLHGFNAARSGDVVVVQEPYKYLVEYQIVATHGAPY
                     SYDTHVPVILMGPGLTAGRYANAATPADIAPTLAALLRVQPPSNSVGRVLNEAVR"
BASE COUNT          651 a         1672 c         1868 g          766 t
ORIGIN      
        1 cgaggaacgg cgagtcgtag aggatgtcga agctctcggc gcggaacgtg ttgcgctcgc
       61 ccgagacggc ggcgaggccg gtggcgatct tccagccttc gcgcggcacg acgcgcacgg
      121 tcgagggcgc ggcgaggtgc ccttcggggt acatcagcag cgccgagttg ttccagaagg
      181 cgtggcggtc gttcagctcg ttcgtccgca cggtcagctc gttcgcgtag acgctgtagc
      241 gcacgcgcac gtcgcgcgcg cccgccgtct cgacgcgcca cgtgttcttg ttcgccttgc
      301 tccattgcag cgcccggccc gcgccgtcct gcgccgcgaa gtcctgcacg tggcgctcga
      361 actcgcgcac caagtaagag ccgggcgtcc agaccggcat caccagctcg gcgcgcgaag
      421 gcggggctgc gccctcgcgc cagcgcagcc gcgcctcgac ctcaaggagg tgcgtgtgcg
      481 gctgcgacat cgagacggtg tagctgatct ccggcgctgt gcgatcttgc gcacgcgccg
      541 gggcggagac gaagagagcg agtacgaacg cagccgcgag ggcgacgtac agacgtgtgt
      601 gacgcatcgt gttcgacact tcctttgccc tgtccgtggg tgttagcggg agaaaactcg
      661 tggcgcgcat cttactacga aacggcgcgg cccgcgacag ccgccgaccg tcgctccgag
      721 tgctcgccgc cgccctgccg ccgcggtttg ccagcgcgat ttcagaagtt gtatgttcgg
      781 agccggacgg cggagcgact gttatcgagg gaggtgggcg gcgatggcga ttgtgacgtg
      841 tgagaagtgc ggcgcgaaga atcgcgtgga cgagggagcg gcggcggcga gccagccggt
      901 gtgcggcagg tgcggcgcga agctggaggc gggcgcgggc ggccacggcg gcggcgcgtc
      961 cggcgacggg aagcctctga cggtgacgga cgagagcttc gcgcgcgagg tggtcgaggc
     1021 gtccgcggag cggcccgtgc tgctcgactg ctgggcgccc tggtgcggcc cgtgccggat
     1081 gatcgcgccg gcgctcgacg agctggccgc cgaatccggc gggcgctacc gcatcgccaa
     1141 gctcaacgtg gacgagaacc cgcgcacctc ttcgacgctc ggcatccgca gcatccccgc
     1201 gctcttcgtc tacaaggacg gccagatcgt agaccgcatc atcggcgcgc agcccaagca
     1261 agtgctcgcc gcccggctcg ccgcgcacgt gtgaagcagt aggcagtagg cggtaggcag
     1321 taggcagcaa aagcaagaag ggcgaagaag cttgggaggt aaagtcccaa gcctcttcgc
     1381 ccttcaagcg ataatgccag aggcttgaga gattcactct caagcctctt gcttttactg
     1441 cctactgcct accgcctacc gcctactgct ttattgcacg ttgacggtca cctgcttctt
     1501 gccgcagggc gaggcgaagg cgacgttgaa ggcgccggtc tttttgctgg tcgaggagac
     1561 ggtgaagcgc gaggcgtcgc cgtccgcttc ggagcgcggc tcggcgagga tggtgatgtc
     1621 ggcccagttg ggcgtcgaga cgttgaggcg cgggagccgc gggccggtgt ggtttacgaa
     1681 cctcacgacg agcgtcacgg agccgccgac cgggatcgac acggagcctt cgttgacggt
     1741 caacgcgcat ccgccgcctg cgcccttcgc ttcgcgcacg ggtgccggtc gcgaggtggt
     1801 agacgtggcc gccgtcgtct cggcgggagg cgtcgtctgc tcgggcgtgg gtgtcggcgt
     1861 ctcggtcggc gcgggcgcct cggtcgtggt cggcgccggc gtgggtgtgg gcgcgggtgc
     1921 ttcggtcggc gtcggtgtcg gctccggtgt cggcgacggg gcgggggatg tcgccgggtc
     1981 tgtggccggt gttgtttcgg cgggcgcggg cgtctcggtg ggggcgggcg tcggcgtcgg
     2041 ttcgggcgcg gcggtcttca cgatggaagg gtctgtggcg gtcgtctcgc ccggtggcgg
     2101 cgtggccgtc tcgcccgtcg tcgaggtcga ggccgcggtg atcggctggc ccgttctgtt
     2161 cgcggcgacg gcggtcgggg cgggggccgc gagcagccgt tcgagtcctt cgagcgagcc
     2221 gttgatgcgg cggtagagcg cttcgatgac ggcgaggcgg acggggtcgg gcgcgccgag
     2281 gcggtagctg cggacgtagg cggcgagcgc ctccttctgg ttgccgctcg cgtcgagcgc
     2341 ggcgccgagg tgccactgcg ccgtcttcca ccagatgctg ttctcgggca gcacgctcac
     2401 cgcgcgccgc aggcgcaccg ccgcctcttt cgcgtcgccg ccgacgaaca aggcccaccc
     2461 cgcgaggtct tcgatgcgcc cgcgcatgac caccgacatc agctcgcgcg aggcgtcggg
     2521 cgcggtcgtc gtcatccccg aggcgagggc gcgcgcgcgc acgtcgtaga ggtcttcggc
     2581 gaagagcgcg accgaggcgt gcggcacgcc gagcgcagcg tcgaggccgc gcttcgccga
     2641 ctcggtcgcc tccaccgcga cgcggtgcgc gacgccgccc tgcgtcagac gtttcgccgc
     2701 gtgcagctcg cggaaggcgc gcatcgggtc ttcgcccgcg acgaactccg ccgccgcttc
     2761 cgcggcctcc gcctctttcg ccgcgcccgt cttcgtcgcc tgatgcaaag ccagcagccc
     2821 cttcaggacg cgcgcgttcg tctcggtgtc ggcggccttg aactggaaga ggccggcgcg
     2881 gcgctcgggc gcgagcagct cggtgaacga cgcggagcgc gccgcgatgc cgcccgcgag
     2941 ccgcgtctcg atctgcccgc ctttgatcgt gaaggagcgc gccagctctt ccgccgcctc
     3001 ttcgtagagg ccggcggcgg ccagcacgct cgccagctcg tagtcgagcg tcgggaagcg
     3061 gcccagctcg cgcgcgggcc tcaaagcgcg ctccgcgtcg agcgggcgcc cccgcgcgag
     3121 cagcgcgcgc gacagcgcga cgcgcgacca gacccagcgg aagcgcggct cgagggcgac
     3181 ggcccgctcg gcgagttcca gcgcgagcac gccttcgtcg tgcgccgcgt accagtacga
     3241 agcgccgacc agcagcggca ggttcgacga ctgctccatg agcgcgccct gcaactcgcg
     3301 ctcggcctcc tcgcgcttcc cggcgtcgaa gagcgagagc accagccctg tgcgcgcgcc
     3361 cgtgtctgtg gcgtccgccg ccagccgctc gcggtagagc gcgagcgctt cctcggcttt
     3421 gccggtcgcg cgcaggaggt ctgcgaggct gcgccgtgcg ccgtgcgagg cggggtcgag
     3481 ttcgctcgcg cgggcgaact cgcgggccgc gtcgtcgagg cggagcgaga agcggtaggc
     3541 cgcgccgagc gcgaggtgcg ccgccgccag atcgggcttg agggcgaccg ccgcctcggc
     3601 cacgcgcgct gcttcggtcg cgcgctcgat gcccaagtag aacgcggcca cgctcagcag
     3661 gcgctgcggg ttgtccttga ccttcgcctc gatgcggcgc gcgagttcga gcgccgcttc
     3721 ctgctcgccg aggacgtgga ggttcgcggg cagttgcgag acgacttcgt agaagagctt
     3781 gtcgggcatc gcggcgggcg cgcccgcgac cgccgcgcgg aacaactctg cgcccgccag
     3841 ccggtcgccg ttgcgcagct tctcgtcgcc gagcgccgcg cgcgcgctcg tcaaaaactc
     3901 gacggcgcgc gcacgcgtct ccgactccgc gttcttcttg acgaactctt cgagccgcgc
     3961 gacgcgctcg gcgggcgcga gcttgacgac ctcctcaagc tccgccttcg aagcctcctc
     4021 gtccgccggg gcggccttgg ctttcggcgc ggcggcgccc gacttaggct tggccgcggc
     4081 gggcttcggc ttgggtttct gcgcgggctg cgccgcgacg accgccgcgc aggcgacgac
     4141 ggaaaccgcg gcggcgagga caatggaaag gctcgacgac tggattcgct tcataacttc
     4201 tttgaccgtt cgcggaagag ggatgacgag agccgtgctg ccgtctatcg aaagatgggc
     4261 gacgagagcc gtgccggcat caatcttccc tgtttcttcg atccgccgtc acctgacggc
     4321 ttcgttcagc acgcgcccga cggagttgct cggcggctgc acgcgcagga gcgcggcgag
     4381 cgtgggggcg atgtcggcgg gcgtggccgc gttggcgtag cgcccggcgg tcagccccgg
     4441 ccccatgagg atgacgggga cgtgcgtgtc gtaagaataa ggcgcgccgt gcgtggcgac
     4501 gatctggtat tcgacgaggt acttgtaagg ctcctgcacg accaccacat cgccgctgcg
     4561 cgcggcgttg aagccgtgga gcacgcggcg cgcgacgggg tcggcgggcg agacgccgcg
     4621 cgcggcgagc tgcgtccgcg tgaagaagcg cgcgatgccg ggcacggcga gcgccgcctc
     4681 gcccgcgagc cgttcgacct cttcgtggcc gatgcggtcg cggcgcagcg cctgatggtc
     4741 gaagtagagg ttgttgttgt cgaaggcgag caggtacttc tccgcttcgt cgccgaagcg
     4801 cgcgcgcagg gcgccgcgca cggcgctcgt cacgtccgac ggcttgacgc gcccgccgct
     4861 caacttcatc gccgcggcgt gctcggggac gggcgcgacg ccgtggtcgg cggtgaaggc
     4921 gacgagcgtg tggcgcagcc ccacgcggcg ctcgacc
//
LOCUS       DBNGOH010000627         2257 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3392173, whole genome shotgun sequence.
ACCESSION   DBNGOH010000627 DBNGOH010000000
VERSION     DBNGOH010000627.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2257)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2257)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2257
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3392173"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..889)
                     /locus_tag="AC159P_12225"
     CDS             complement(<1..889)
                     /locus_tag="AC159P_12225"
                     /inference="COORDINATES: protein motif:HMM:NF012471.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HIR3696257.1"
                     /translation="MKRNARLLVAAVAAALLLASLSGADARAQGERADRFDFYTRGPY
                     RENVPRPQSILRYDVGDFHTNYAMMERVITAIAQAAPDRVRVYDIGLTNEYRMQHVVA
                     ISAPENIARLDELRANMKRLSDPRTLSGDEAQRLVSSAPVHVWLAYTIHGNESASFEA
                     MMQVVYQLAASNEPATLDILRNSVALINVCSNPDGHERFVTWYNSFGNGNPDPSAAEH
                     DEPWSVYGRVNRYRFDLNRDNVAVSQAETRNLQRAYLEWNPQVAADHHGQPSQFFFPP
                     AALPVNPNLPPEQTERWLAA"
     gene            1003..1443
                     /locus_tag="AC159P_12230"
     CDS             1003..1443
                     /locus_tag="AC159P_12230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019867848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR3696258.1"
                     /translation="MRELHTEIEIDAPPERVWQVLTDFAAYPEWNPFVTSVRGGGELK
                     EGARLEIVVSVPEGPTMTFKPRVLKVEPARELRWLGTLPVPGLFNGEHIFRLEAAGEG
                     RARFLHGEHFSGLLIPFMGGVLRKTERGYRLMNEALKRRVEAGG"
     gene            1583..1807
                     /locus_tag="AC159P_12235"
     CDS             1583..1807
                     /locus_tag="AC159P_12235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696259.1"
                     /translation="MSSNDYATSGQGDGRCPRCGEGVLRAWYELGEEEREAVRRLPAS
                     ADFTLEERAARHRWCARCWHEEVFGEPRGA"
     gene            1913..>2257
                     /locus_tag="AC159P_12240"
     CDS             1913..>2257
                     /locus_tag="AC159P_12240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014786901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696260.1"
                     /translation="MRYVRALALLCVLLLALLFVLPAATPGSLAQQSGESKPIVIKAA
                     RMFDGRSDRVVSPGVVVVVGGRIQSAGAGGQIPAGAEVIDLGDATLLPGFMDAHTHLS
                     GESSEDWKQDELD"
BASE COUNT          361 a          700 c          825 g          371 t
ORIGIN      
        1 aggcggcgag ccagcgctcg gtctgttcgg gcgggaggtt cgggttgacg ggcagggccg
       61 cgggcgggaa gaagaactgc gagggctggc cgtggtgatc ggcggcgacc tgcgggttcc
      121 attcgaggta ggcgcgttgc aggttgcgcg tctcggcctg cgagacggcg acgttgtcgc
      181 ggttgaggtc gaaacggtag cggttgacgc gaccgtagac cgaccacggc tcgtcgtgct
      241 cggcggcgga agggtccggg ttgccgttgc cgaacgagtt gtaccaagtg acgaagcgct
      301 cgtggccgtc ggggttcgag cagacgttga tgagggcgac gctgttgcgc aggatgtcga
      361 gcgtcgccgg ctcgttcgac gcggcgagct ggtagacgac ctgcatcatc gcctcgaagc
      421 tggccgactc gttgccgtgg atcgtgtagg cgagccacac gtggacgggc gcggaagaga
      481 cgagccgctg cgcctcgtcg cccgacaaag tgcgcgggtc ggagaggcgc ttcatgttcg
      541 ccctcagctc gtcgagccgc gcgatgttct cgggcgcgct gatggcgacg acatgctgca
      601 tccggtactc gttcgtcagc ccgatgtcgt agacgcggac gcggtcgggc gcggcctgcg
      661 cgatcgccgt gatgacgcgc tccatcatcg cgtagttggt gtggaagtcg cccacgtcgt
      721 agcggaggat gctctgcggg cgcgggacgt tctcgcggta agggccgcgc gtgtagaagt
      781 cgaagcggtc ggcgcgctca ccttgcgcgc gcgcgtcggc gccggagagg gaggcgagca
      841 gcagcgccgc ggcgacggcg gcgacgagca ggcgggcgtt tctcttcatg tgttggaggc
      901 gaactttcca tttccggtca ggtgtgttaa agacagcgcg atattaacgc gcgggcggca
      961 cgattctcaa attctttcac gcgccccgcg cgccgaaaga acatgagaga gcttcacacc
     1021 gagatcgaaa tcgacgcgcc gcccgaacgc gtctggcagg tgctcacgga cttcgcggct
     1081 tacccggagt ggaacccgtt cgtgacgagc gtgcggggcg gcggcgagtt gaaggaaggc
     1141 gcacggctcg aaatcgtcgt cagcgtcccc gaagggccga cgatgacctt caagccgcgc
     1201 gtgctcaagg tcgagccggc gcgcgagctg cgctggctcg gcacgctccc cgtgcccggc
     1261 ctcttcaacg gcgagcacat cttccggctc gaagccgcgg gcgaagggcg cgcgcgcttc
     1321 ctccacggcg agcacttctc gggcctcctc atccccttca tgggcggcgt gctcaggaag
     1381 accgagcgcg gctaccggct gatgaacgaa gcgctcaagc ggcgcgtcga agcgggcggc
     1441 taacaactcc tgtaggggca gggcaagtcc tcggcccgcg ttgcgtgacg actcgtcgct
     1501 tgaactaaaa cggacgccgg gccgcggaca agccctgccc ctacaggctc gttcatcgca
     1561 cttcattcaa agggaacgtt gcatgagcag taacgattac gcgacgagcg ggcagggcga
     1621 cgggcggtgc ccgcgctgcg gcgagggcgt cttgcgcgcg tggtacgagc tgggcgagga
     1681 ggagcgcgag gccgtcaggc ggcttccggc ttccgccgac ttcacgctcg aagagcgcgc
     1741 ggcgcgtcac cgctggtgcg cgcgctgctg gcacgaggaa gtgttcggcg agccgcgcgg
     1801 cgcgtgagtc gtccgtttca ggttcgaaat gcgacagcct gaatttcgca tccgcgatct
     1861 cccttgcggc gtctttcacg aagttccaac acaccacagc cggaggccac acatgagata
     1921 cgttcgtgct ctcgccctgc tctgcgtcct gctcttggca ctgctcttcg tcctgcccgc
     1981 cgcaaccccc ggaagcctcg cgcaacagag cggcgagtcg aagcccatcg tcatcaaggc
     2041 cgcgcggatg ttcgacggca ggagcgaccg cgtggtgtcg cccggagtgg tcgtcgtcgt
     2101 gggcgggcgg attcagtctg cgggcgcggg cggccaaatt cccgcgggcg cggaggtgat
     2161 cgacctcggc gacgcgacgc tgctgcccgg cttcatggac gcgcacaccc acctgtcggg
     2221 cgagtcgagc gaggactgga agcaggacga actggac
//
LOCUS       DBNGOH010000628         3179 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_641935, whole genome shotgun sequence.
ACCESSION   DBNGOH010000628 DBNGOH010000000
VERSION     DBNGOH010000628.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3179)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3179)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3179
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_641935"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..752)
                     /locus_tag="AC159P_12245"
     CDS             complement(<1..752)
                     /locus_tag="AC159P_12245"
                     /inference="COORDINATES: protein motif:HMM:NF053443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696261.1"
                     /translation="MEVRGTKGVSDSSPVKLISSTRADSGPQYSPDGRKVVFSSSHTG
                     PLEVWACEADGSNPVQVTSFGGPHVGSPRWSPDGRQIVFDSPAEGHRDIYVVGVDGGK
                     PRRLTVEASADVRPSWSRDGRFVYFGSDRGGGWQVWKAPAEGGATIQLTKGGGREAFD
                     SPDGKFVYYTKADAPGLWRVPAPVIRPTLSGPDRLMGIGCAGGHNELRCDAGCRQRVD
                     GARRKAAGKKGHVVFLTHAAGSCRFPSSSVFCR"
     gene            complement(930..1349)
                     /locus_tag="AC159P_12250"
     CDS             complement(930..1349)
                     /locus_tag="AC159P_12250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012876643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HIR3696262.1"
                     /translation="MSAQQPRQTKFGFSKTVTVPFEQALEKTRSALQTEGFGVLSEID
                     IQGKLKEKLGVDFRRYTILGACNPPLAYKALREELDLGLLLPCNVVVYEGEEAGETVV
                     AAVDAGAMLSVAGGGETLREVSGEVNERLRRVLDMID"
     gene            complement(1685..2020)
                     /locus_tag="AC159P_12255"
     CDS             complement(1685..2020)
                     /locus_tag="AC159P_12255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011143941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HIR3696263.1"
                     /translation="MAKAKKRPMTPEALALVAARFKVLAEPLRLRILQALEGGEMSVG
                     DITEAVESTQPNISKHLKMLQEAGVVSRRQEGNTVYYSIADETVFEICDVVCSSIRER
                     LQAQAGVFA"
     gene            2222..>3179
                     /locus_tag="AC159P_12260"
     CDS             2222..>3179
                     /locus_tag="AC159P_12260"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012790.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000098 - sulfur amino acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HIR3696264.1"
                     /translation="MIRKNGVGVTVRDTTRWALAVLLACGAVAGLHVAGRPRAEGQGL
                     RRARVPEAGVRAEMLVSTDWLGARLNDPRVAVLHVARERAHYEQGHVPGAHFVALGEI
                     ASSRGGVPNELPPAAELRKLFERLGVGDAGRVVLYGDNAGLSAARAYFTLDYLGHGAR
                     ASLLDGGLEKWQAERRRVSTASAETKSRPFTPRVRAEAVTDRDVVRDLSWVAANVPSS
                     ATVLIDARPAEEYTGAKPGEGVPRGGHIPGAANVFWMQHLVSRESPVMRPAAELRRLY
                     EAAGARRGVKVVTYCRTGVQASHAYFTAKYLGYDVVMYDGSFI"
BASE COUNT          522 a         1075 c         1050 g          532 t
ORIGIN      
        1 cggcagaaca cgcttgacga agggaatcga caggaacctg cagcatgagt gaggaacacg
       61 acgtgaccct ttttccccgc cgcttttctc cgagcgccat ctacgcgttg acggcagccc
      121 gcgtcacatc gcagctcgtt gtgaccgccg gcgcaaccga ttcccataag acgatcaggc
      181 ccggagagag ttggccggat gaccggggcc ggcacccgcc acagccccgg cgcgtccgcc
      241 ttcgtgtagt agacgaactt gccgtcgggg gagtcgaatg cctcgcgccc gccacctttc
      301 gtcaactgta tagttgcccc gccttccgcc ggagccttcc acacctgcca cccgccgccg
      361 cggtccgagc cgaagtagac gaacctcccg tccctcgacc aactggggcg cacatccgcc
      421 gaagcctcga ccgtcaggcg gcgcggcttg cctccgtcca caccgaccac gtaaatgtcc
      481 cggtggccct cggcgggaga gtcgaagacg atctgccgtc cgtccggaga ccagcgcggg
      541 ctgccgacgt gcgggccgcc gaaggaggtc acctggacgg ggttcgagcc gtccgcctcg
      601 cacgcccaga cctctaaagg gccggtgtgc gaagatgaga agacgacctt ccgcccgtcc
      661 ggggaatact gggggccgct atccgcccgc gtggatgaga ttaactttac cggcgaactg
      721 tcgctgacgc ccttcgtgcc ccggacctca atctaaagca tcaacacgcc gccgcgttgg
      781 aagtttccgt tggacggaga gatcgacatc acgcccggtt tgcagtgctg cctgatttgc
      841 gagggcgagc agcaccccga aagagtgcag ggtgaagaag ggcgtctcga acaggcgagg
      901 gtacacccga aaacttcgac catcttccct taatcgatca tgtcgaggac ccgccgaagg
      961 cgctcgttga cctcgcccga cacctccctg agcgtctcgc cgccgccggc gaccgagagc
     1021 atggccccgg cgtccacggc cgcgaccacg gtctcacccg cctcttcgcc ctcgtagacg
     1081 acgacgttac acgggaggag cagcccgagg tcgagctctt cgcggagggc cttgtaggcg
     1141 agcggcgggt tacacgcgcc gaggatggtg tagcggcgga aatcgacgcc cagcttctcc
     1201 ttgagcttgc cctgaatgtc tatttccgag agcacgccga aaccctcggt ttgcagcgcg
     1261 gagcgcgtct tctccagtgc ctgctcgaag ggtacggtca ccgtcttgct gaacccgaac
     1321 ttagtctgcc gaggctgctg cgcactcatc tttcaccctc cttcaaggac gtatctcttc
     1381 accgaagacc gtacggctta tggccggcgc ccgctttcgg cgcccgccga tggctcgcct
     1441 gcgggtcgtg tcccggccgg gccgtcccgc cgtgtcgaat cgcgccggaa ctcgacgcgg
     1501 catgaattcg acattcaggt gggcaccccg aaatacggga gtacccaacg catcacgacg
     1561 aagtacgccg cgatgaacag gacgggccat aaccatacgg ccattttcag aaccctcccc
     1621 cttctcgtag acatgaacaa ctgcgcggcg cgcggccgac tccgagcttc ctcccggcgg
     1681 cgcctcaggc gaagaccccg gcttgcgctt ggagccgctc gcggatgctc gagcagacga
     1741 cgtcgcagat ctcgaagacg gtctcgtcgg cgatggagta gtagacggtg ttaccctcct
     1801 gacggcggct gacgacgccg gcctcctgga gcatcttgag gtgcttgctg atgttcggct
     1861 gcgtggactc gaccgcctcg gtgatgtcgc ccacgctcat ctcgccgccc tcgagcgcct
     1921 ggaggatgcg caggcgcagc ggctcggcga gcaccttgaa gcgggcggcg accagcgcca
     1981 gcgcctcggg ggtcatcggt ctcttcttcg ccttcgccat ggcctctcct tgccggttga
     2041 caggagtgct atctagtaat atggtaaaac tcgcaatcta ttataaaagc tccgtgggcg
     2101 atccgcaagc ggtgcgtctg tgtctgaagc gtcgccgccc gcggcgcggg cggcgggcgg
     2161 cgtgagcgcc ggccccgctc ggagagctga ttgggtgcgc agcgcccgag gaggagatga
     2221 catgattcgg aaaaacggtg tgggcgtcac ggtgagggat acgacccggt gggccttggc
     2281 cgtcctgctc gcctgcgggg cggtggccgg cctgcacgtc gcggggcggc cgcgcgcgga
     2341 aggccagggt ttacggcgag cgagagttcc cgaggccggc gtgcgtgcgg agatgctcgt
     2401 ctcgaccgac tggctcggcg cccgtcttaa tgacccgcgg gtggccgtcc tgcacgtcgc
     2461 gcgcgagcgc gcacactacg agcaggggca cgtcccgggc gcgcacttcg tcgccctggg
     2521 cgaaattgct tcgtcgcgcg gcggcgtgcc gaatgagttg cctcccgccg cagaacttcg
     2581 gaaactcttc gagcggctgg gggtgggcga cgcgggccgc gtcgtgcttt acggcgataa
     2641 cgccgggctg tcggcggccc gcgcctactt cacgctcgac tacctggggc acggtgcccg
     2701 cgcgtccctg ctagacggcg gcctggagaa gtggcaggcc gaacgccgca gggtctcgac
     2761 cgcgtcggcc gaaaccaagt cgcggccctt tacgcccaga gtgcgcgcgg aagccgttac
     2821 ggatcgggac gtcgtgcgcg acctgtcgtg ggtggctgcg aacgtgccct cctcggccac
     2881 ggtgctgatc gacgcgcgcc cggccgaaga gtacacgggc gctaagccgg gcgagggcgt
     2941 gccgcgcggc ggccacatcc ccggggcggc gaacgtcttc tggatgcagc acctcgtcag
     3001 ccgtgagagt cctgtgatgc gcccggcggc cgaactgcgg aggctctacg aggcggccgg
     3061 cgcccgacgc ggcgtgaagg tcgtgacgta ctgccgcacc ggcgtccagg cctcgcacgc
     3121 atacttcacc gccaagtatt tgggctacga tgtggtcatg tacgacggct ctttcatcg
//
LOCUS       DBNGOH010000629         2996 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_459944, whole genome shotgun sequence.
ACCESSION   DBNGOH010000629 DBNGOH010000000
VERSION     DBNGOH010000629.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2996)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2996)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2996
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_459944"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2397
                     /locus_tag="AC159P_12265"
     CDS             <1..2397
                     /locus_tag="AC159P_12265"
                     /inference="COORDINATES: protein motif:HMM:TIGR00914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR3696265.1"
                     /translation="NGKEAVGGVVIARYGVSALEAIEAVKRKVEELKPGLPEGVRFVP
                     FYDRTQLINRAAGTLREALIEELVLVTLVNLIVLAHFRSVLVVTIPLPLAVLVSFLFM
                     RYFGITSNIMSLAGIAIAISDLVDAGIVVTENAYRAIERDNIDTKDKRLVRRVVLDAT
                     KKVGVPIFASMAIIILAFAPVFALTGQEGKLFHPLAFTKTFAMVGAAFMAVTLVPVLC
                     TYFLGGKLHPEEWNPLMRGLRAVYRPTLVGALRHRLITVSVALVFFAGAVYVSTRIGS
                     EFMPPLNEGDLMFMPVTDPAISMGEALRIMQRQDEILKGFPEVEWAVGKAGRAETSTD
                     PSPVNMNETIVHLRPEAEWREGVTREKLVAEMDAALRMPGVTNIWTQPIINRIEMLAT
                     GMRSQVGIKVFGNDLDTLEETSRRIADVLKSVPGAADVYPEQIGGAPYVDIKINRTAA
                     ARYGIDTGTIQDAIERGIGETNLTVTIEGRRRFPIRVRYAPEFRSSPEAIGNIPITSP
                     AGAPIPLAQLAEIRTVEGPAMIQSENGLLRGTVLLNVRGRDIGSFVEEAKHHLAAQVQ
                     MPAGYYLQWSGQYENQERARKRLLIVVPVVIVIIFGTLYLTYHSALEAAHVLLAVPFA
                     LTGGFYLLWLLDYNFSVAVWVGFIALFGAAVQTGMVMVVYLEEAVERKRNELGRLTRE
                     TLLEAVTEGALLRLRPKVMTVSTIVAGLLPIMWSTSAGSEVMKPLATPVFGGMVSSLL
                     HVLIVTPVIFYWLRLRELKKGAAAAGGEGRGRFESGPRGRVPVNGESPGAEVEEVVHR
                     "
     gene            2384..2860
                     /locus_tag="AC159P_12270"
     CDS             2384..2860
                     /locus_tag="AC159P_12270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696266.1"
                     /translation="MSTADPRRFARRFAAILFAVSFAVNWVWEMSQMSAYAPMPGHTW
                     KEVTLFCTAASVGDAVLTLLIVGAGALVARRRSWVAAFGWKAYAGAAFLGGVSAVVIE
                     WAAVGSGYWSYAGAMPRVPLLEVGLWPFAQLALLVPFSLWAAGRLVDRFTGSGSHV"
BASE COUNT          456 a          993 c         1023 g          524 t
ORIGIN      
        1 aacggtaagg aggccgtggg cggggtcgtc atcgcgcgct acggcgtgag cgcgctcgag
       61 gccatcgagg ccgtcaagag gaaggtcgag gagctgaagc ccggcctgcc cgaaggcgtc
      121 cgcttcgtcc cattctacga ccgcacgcaa ctcatcaacc gcgccgcggg cacgctccgc
      181 gaggccctca tcgaagagct ggtactggtt acgctggtca acctgatcgt cctcgcgcac
      241 ttccgctcgg tgctcgtcgt cacgatcccg ctcccgctcg ccgtgctcgt ctcgttcctc
      301 ttcatgaggt acttcgggat cacctcgaac atcatgtcgc tggcgggcat cgccatcgcc
      361 atctcggacc tggtggatgc ggggatcgtc gtcaccgaga acgcctaccg cgccatcgag
      421 cgtgacaaca tcgacacgaa ggacaagcgg ctcgtccgtc gcgtcgtgct cgacgcgacg
      481 aagaaggtcg gcgtccccat cttcgcctcg atggcgatca tcatcctcgc cttcgcgccc
      541 gtcttcgcgc tgacggggca ggaggggaaa ctctttcacc ccctcgcctt cacgaagacg
      601 ttcgccatgg tcggcgcggc cttcatggcc gtgacgctcg tccccgtgct ctgcacctac
      661 ttcctcggcg gcaagctcca ccccgaggag tggaacccgc tgatgcgcgg cttgcgggcc
      721 gtctacaggc cgacgctcgt aggcgccctg cgccaccgcc tgatcacggt gtcggtcgcg
      781 ctcgtcttct tcgccggcgc ggtctacgtc tcgacgcgca tcggcagcga gttcatgccg
      841 cccttgaacg agggcgacct gatgttcatg cccgtcaccg acccggcgat ctccatgggc
      901 gaggccctgc ggatcatgca gaggcaggac gaaatcctga agggcttccc cgaggtcgag
      961 tgggccgtcg gcaaggcggg ccgcgcggag acttccaccg acccgtcgcc ggtgaacatg
     1021 aacgagacca tcgtccacct caggcccgaa gcggagtggc gcgaaggcgt gacgcgcgag
     1081 aagctggtcg ccgagatgga cgcggctttg aggatgccgg gtgtcaccaa catctggacg
     1141 cagccgatca tcaaccgcat cgagatgctg gcgacgggga tgcgctcgca ggtcggcatc
     1201 aaggtcttcg gcaacgacct ggacacgctg gaggagactt cacggaggat cgcggacgtg
     1261 ctcaagtccg tgccgggcgc ggcggacgtt taccccgaac agatcggcgg cgcgccctac
     1321 gtggacatca agatcaaccg gacggccgcc gcccgctacg gcatcgacac gggaacaatt
     1381 caggacgcga tcgagcgcgg gatcggcgag acgaacctga cggtgacgat tgaagggcgc
     1441 cgccgcttcc cgatccgcgt gcggtacgcg ccggagttcc gcagctcgcc cgaagccatc
     1501 ggaaacattc ccatcacgtc gcccgcgggc gcccccatcc cgcttgcgca actcgccgag
     1561 attcgcacgg tcgaagggcc ggccatgatc cagagcgaga acggactttt gcgggggacg
     1621 gtgctcctga acgtgcgcgg gcgcgacatc gggagcttcg tcgaggaggc gaaacaccat
     1681 ctggccgcgc aggtgcagat gcccgccggc tattacctcc agtggagcgg gcagtacgag
     1741 aaccaggagc gcgcacgcaa gcggctgttg atcgtcgtcc ccgtcgtcat cgtcatcatc
     1801 ttcggcacgc tctacctgac ctaccactcg gccctcgaag cggcgcacgt cctcctcgcc
     1861 gtcccgttcg cgctgaccgg cgggttctac ctgctgtggc tcctcgacta caacttttcg
     1921 gtcgccgtct gggtcggctt catcgcgctc ttcggggcgg cggtgcagac cggcatggta
     1981 atggtggtct acctggagga ggcggtcgag cggaagagga atgagctcgg ccggctgacg
     2041 cgggagactt tactcgaggc cgtgacggag ggcgcgctgc tccggctccg gcccaaggtg
     2101 atgacggtct cgaccatcgt cgcgggcctg ctcccgatca tgtggagcac gagcgcgggg
     2161 tcggaggtga tgaagccgct ggcgacgccc gtcttcggcg ggatggtgtc gagcctcctg
     2221 cacgtgctca tcgtcacccc ggtcatcttc tactggctac gcctgcgcga gttgaagaag
     2281 ggggcggccg cggcgggcgg cgaggggcgc gggcggttcg agtcagggcc gcgggggcgg
     2341 gtgccggtga acggcgagtc gcccggagcg gaggtcgaag aagttgtcca ccgctgaccc
     2401 acggcgcttc gcccggcgct tcgcggcgat cctgttcgcc gtctcgttcg ccgtcaactg
     2461 ggtttgggag atgtcgcaga tgtcagccta cgcgccgatg ccggggcaca cgtggaagga
     2521 agtgaccctc ttctgcaccg ccgcttccgt cggggacgcg gtgctcacac tcctcatcgt
     2581 cggggccggc gcgctggtcg cgcgccgtcg gtcgtgggtc gcggcgttcg gttggaaggc
     2641 ttacgcgggg gcggcgttct tgggcggagt ttcagccgtg gttatcgagt gggcggcggt
     2701 cggctccggc tattggtctt acgccggcgc gatgccccgt gtccccctac tcgaagtcgg
     2761 tctctggccc ttcgcgcagc tcgcgctgct cgtacccttc tcgctgtggg ccgccgggcg
     2821 tttagtcgac cggtttaccg ggagtggcag tcatgtctaa agtcctgaga ttgagaacag
     2881 tagggttgag ggcgctcgcc gtcttcgggc tcgtcgccgt ccaggcgtta cacggcgcgt
     2941 cgtccgcgct cgcacagtcg tcgccacccg ccgcggagac gggcgtcgtc gcggcg
//
LOCUS       DBNGOH010000630         1973 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1010661, whole genome shotgun sequence.
ACCESSION   DBNGOH010000630 DBNGOH010000000
VERSION     DBNGOH010000630.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1973)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1973)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1973
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1010661"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..873)
                     /locus_tag="AC159P_12275"
     CDS             complement(<1..873)
                     /locus_tag="AC159P_12275"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HIR3696267.1"
                     /translation="MNPRKPIMLMLAAGLVVVLALTFTPYALLSRARHAVETLVRPAT
                     DSGALKEVEAERLTPPFDVAAWYAGRAVDADGHGVLVETLDGRQVFAAHNADTPFNPA
                     SLVKLATTLVVLRRLGKDYRFETRFYIEGEADKSGALRGRLIVAGSDPTFGDIAAVVV
                     REELEKRGVKSVPEVIAVSPDFTFNFSGKPEESAERLAKVMKFETKKFAVAAAPAGQP
                     TFVMRSYPLRAILLYMNAHSSNFVAERLGALVGGAAGVQDYLVGELQIPADRVTLSTT
                     SGLEHNRLTPRGLIT"
     gene            999..1886
                     /locus_tag="AC159P_12280"
     CDS             999..1886
                     /locus_tag="AC159P_12280"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: protein motif:HMM:NF013632.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HIR3696268.1"
                     /translation="MLDITAQQALSGTDATAGLPFLFVHCAVGAFGAIVGSLLNVVIH
                     RVPRDESVAFPASHCPNCNTAIKPYDNVPVLSWLALRGRCRACRAPISPRYPAVEALT
                     AALFALTFRLHSGLSLSLPFDLVFVAALVALVFIDAEHMILPDLITLPGVAFALVARV
                     VVPNLYGVGFVAAEYPQLPPWALSLIGAVLGALVGGGFLWVVGWLWERARGVEAMGLG
                     DVKMMFMVGAFLGWPLTVLTIFFGVLTGSIVGVALMLRRGERDMQMLLPFGIFLGLGA
                     LVSLLVGQPIAEWYAGQFR"
BASE COUNT          278 a          685 c          643 g          367 t
ORIGIN      
        1 cgtaatcaac ccgcgcggcg taaggcggtt gtgttcgagg cccgaggtgg tcgagagcgt
       61 cacgcggtcg gcgggaattt gcagctcgcc cacgaggtag tcctgcacgc ccgccgcgcc
      121 gccgaccaaa gcgccgagac gttcggcgac gaagttgctc gaatgcgcgt tcatgtagag
      181 caggatggcg cgcagcgggt acgagcgcat cacgaacgtc ggctgcccgg cgggcgccgc
      241 ggcgacggcg aacttcttcg tctcgaactt catcaccttc gccaggcgct cggcggactc
      301 ctcgggcttg ccgctgaagt tgaaggtgaa gtccggcgag acggcgatca cttcgggcac
      361 gctcttgacg ccgcgctttt cgagttcttc gcggacgacc acggcagcga tgtcgccgaa
      421 cgtcgggtcg ctcccggcga cgatcaagcg cccgcgtagc gcgcccgact tgtccgcctc
      481 gccctcgatg tagaagcgcg tctcgaagcg gtagtccttg ccgagcctgc ggagcacgac
      541 gagcgtggtc gccagcttga cgagcgacgc ggggttgaac ggcgtgtcgg cgttgtgcgc
      601 ggcgaagacc tgccgaccgt cgagcgtctc gacgaggacg ccgtggccgt ccgcgtccac
      661 ggcgcggccc gcgtaccacg ccgccacgtc gaacggcggc gtcaaccgct cggcctcgac
      721 ctctttgagg gcgccgctgt ctgtagcggg ccggactaag gtttcgacgg cgtgtcgcgc
      781 gcgagagagc agcgcgtagg gcgtgaaggt cagtgcgagg acgacgacga ggccggcggc
      841 cagcatcaac atgatcggct tgcgtgggtt caagctgtca ttaccttcct gctcgaaatt
      901 atcggccccg gtcttccgca aagagaccac ttggctttaa tatgcggaag gccgtccggc
      961 gcgtcaagcg agcgcgcccg ccgcggctca caccattcat gctcgacatc acagcccaac
     1021 aagcactctc gggaacggac gcgactgccg gactcccctt cctcttcgtc cactgcgcgg
     1081 tcggcgcctt cggcgccatc gtcgggagct tactaaacgt cgtcatccac cgcgtcccgc
     1141 gcgatgagtc cgtagctttc cccgcgtcgc actgccccaa ctgcaacacg gcgatcaagc
     1201 cttacgacaa cgtgcccgtc ctgagctggc tcgcgctgcg cggtcgctgc cgcgcgtgcc
     1261 gcgccccgat ctcgccgcgc tatccggcgg tcgaggcttt gaccgccgcg ctcttcgcgc
     1321 tgaccttccg gctccactca gggctgtcgc tctcgctccc cttcgacctc gtcttcgtcg
     1381 ccgccctcgt cgcgctcgtc ttcatcgacg ccgagcacat gatcctgccc gacctgatta
     1441 cgctgcccgg cgtcgccttc gcgctcgtcg cgcgcgtcgt cgttccgaac ctctacggcg
     1501 tcggcttcgt cgccgccgaa tacccgcaac tgccgccgtg ggccctctct ctgatcggcg
     1561 ctgttctggg cgcgctcgtc ggcggcggct tcctgtgggt cgtcggctgg ctctgggagc
     1621 gcgcgcgcgg cgtcgaggcg atgggcctcg gcgacgtgaa gatgatgttc atggtggggg
     1681 ccttcttggg gtggccgctg acggtgctga cgatcttctt cggcgtcctc accggctcca
     1741 tcgtcggcgt cgcgctcatg ctgcggcgcg gcgagcgcga catgcagatg ctcctcccct
     1801 tcggcatctt cctcggcctc ggcgccctcg tcagcctcct cgtcggccag cccatcgccg
     1861 aatggtacgc gggacagttc cgttgaaagt gctgatgact gagggctgag tgatgagtga
     1921 cgagtgacga gtgacgagtg atgcgagccg cgaagatcat tctctgtctc ctc
//
LOCUS       DBNGOH010000631         4157 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2842579, whole genome shotgun sequence.
ACCESSION   DBNGOH010000631 DBNGOH010000000
VERSION     DBNGOH010000631.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4157)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4157)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4157
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2842579"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..388)
                     /locus_tag="AC159P_12285"
     CDS             complement(<1..388)
                     /locus_tag="AC159P_12285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020711830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696269.1"
                     /translation="MKHPLTHDITRKRIFPLVVCCLLLLLGGCRERKAGGGGGDGPSV
                     ELIKVPMTDAGGGPELGTIEGRVRGAREGRRVVLYARSGAWYVQPFADQPFTMIQPDA
                     TWRNSTHLGTEYAALLVEPGYSPPART"
     gene            737..1372
                     /locus_tag="AC159P_12290"
     CDS             737..1372
                     /locus_tag="AC159P_12290"
                     /inference="COORDINATES: protein motif:HMM:NF025597.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4339 domain-containing protein"
                     /protein_id="HIR3696270.1"
                     /translation="MEVYVYRNNRQEGPYEEAAVAEGLRSGSLSPDDLARFVGSDRWA
                     PLGSLFPHLLRAPQPPPLPPPAHTWMDDSATGRPARPVEPLRPGAHPYAPPVQYVPPP
                     VPVPPPHTLAAQQQAARPYGGAKPGGLPAGAMSMSVVALCLMLVGLIPCLGWLNWLTI
                     LIGVIAHLLSWVAVFTEKNEDARSKAVIGLVVSFVAVFIGGIRLVLGFGCL"
     gene            1385..1735
                     /locus_tag="AC159P_12295"
     CDS             1385..1735
                     /locus_tag="AC159P_12295"
                     /inference="COORDINATES: protein motif:HMM:NF022281.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2752 domain-containing protein"
                     /protein_id="HIR3696271.1"
                     /translation="MDENLGRMFVWPRAREWWEALRWVLLVFTLLVLVSLLGSFVSYE
                     RVVAEGHPWLPRAYHCAGCPLCGMTRSFCALSSGRWHEAVRWNRGGPALYAAGWLWLI
                     TATAVVTRRLLGGR"
     gene            complement(1856..2413)
                     /locus_tag="AC159P_12300"
     CDS             complement(1856..2413)
                     /locus_tag="AC159P_12300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HIR3696272.1"
                     /translation="MEKIRNGFFALALALSASAVLAADANAQGRSVDRAQVEKKVRRE
                     LVTLPYYGVFDNLAYEVNGGTVTLYGQVVRPSTRSDAERRVARLAGVARVVNRIEVLP
                     LSGFDDSIRARTYRALFGADSPLFRYGRGTNPSIHIVVNRGHVTLEGVVANEGDSRLA
                     YIRARGVSGVFSVTNNLDVERRRGR"
     gene            2607..3176
                     /locus_tag="AC159P_12305"
     CDS             2607..3176
                     /locus_tag="AC159P_12305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR3696273.1"
                     /translation="MNGQGSDNITRLLIKLTNGDAAAVDALLPHIYDELRRHAASYLR
                     RERPDHTLQPTALVHEAYLRLVDQTQVQWQNRAHFFGVAAQMMRRLLVDHARAHNAEK
                     RGHDFQKLSIDENIDKAVERSAELLALDDALRELAEIDPQKARLVELRYFGGLSVEET
                     AEVLGVSAPTVKRHWRATRAWLFGRMRQP"
     gene            3315..>4157
                     /locus_tag="AC159P_12310"
     CDS             3315..>4157
                     /locus_tag="AC159P_12310"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HIR3696274.1"
                     /translation="MTPERWRQVEEIFQTALDLAPGERAPFLEEACGGDAELRRDVEA
                     MLAQYEEAGDFLDEPLVEQSGLQELAALMGEADKTDPLIGQRVGAYRIEREIGRGGMG
                     VVYLAQRADGAFRMRAAVKVIKRGMDTDFVLRRFRHERRILASLQHYNIARMFDAGAT
                     EAGLPYFVMEYIEGEPLYDYCDARQLPLRERLRLFAEVCDAVHYAHQKQVVHRDIKPP
                     NILVNPSGVPKLFDFGIAKLLNPELTPDTAPQTLTAMRLMTVEYASPEQVQGLPVTSL
                     SDVYS"
BASE COUNT          676 a         1418 c         1390 g          673 t
ORIGIN      
        1 ccgtcctggc cggcggactg tagcccggct cgacgagcag ggccgcgtac tccgtcccga
       61 ggtgggtcga gttccgccac gtcgcgtcgg gttgaatcat cgtgaacggc tggtcggcga
      121 aaggctgcac gtaccacgcg ccgctccgcg cgtagagcac gacccgccgg ccttcgcgcg
      181 cgccccggac gcgcccctcg atggtgccga gttccggccc gccgcccgcg tccgtcatcg
      241 gaactttgat caactcgacg gaggggccgt cgccgccgcc gccgcccgcc ttccgctcgc
      301 ggcaaccgcc gagcagtagc agcagacaac agacgacgag cgggaagatt cttttccgtg
      361 tgatgtcgtg cgtcaaagga tgcttcacct gtcgatgcgt ggcggcgaaa gaaagcgaga
      421 gccgcgcgcg cggctggtcg cgtccgtgcg cggaggttca tgggtgggac tgaacaggag
      481 tggccgcccc tcgtgtgtca aacgggatgc ccgcacgggc gtcgcgtatt gtgtccacat
      541 cggtcgataa aatcaacgcc gggcgtcccc gtcgaaacgc aaacaaagcc ttgacgccgg
      601 gcgggatcgg tgtaagctac tgcgcctgac cgcagagggc cagagagaga ccggacgcct
      661 cacgtcgaaa tcgcacacac gccaacccga atagttcaag acccttcgcc gaaggcacgg
      721 gcggcaagga gcagcgatgg aagtttacgt ctacaggaac aaccggcagg aagggccgta
      781 cgaagaggcc gccgtcgccg aagggctgcg cagcggctcg ctctcgccgg acgatttggc
      841 gcgcttcgtc ggctccgaca ggtgggcgcc gctcggctct ctgttcccgc acctgctgcg
      901 cgcgccacag ccgccgccgc tgccgccgcc cgcacacact tggatggacg actctgcgac
      961 gggacgaccc gcgaggccgg tggagccgct gcgtccgggc gcgcatcctt acgcgccgcc
     1021 cgtccagtat gtgccgccgc cggtgcccgt gcctccgccg cacacgctcg cggcgcagca
     1081 gcaggcggcg aggccgtacg gcggggcgaa gcccggcggc ctgccggcgg gcgcgatgtc
     1141 gatgagcgtc gtggcgttgt gcctgatgct cgtcgggctg attccgtgtt tgggttggct
     1201 caactggttg acgatcctca tcggcgtcat cgcgcacctg ctgtcgtggg tcgccgtctt
     1261 cacggagaag aatgaggacg cacggagcaa ggccgtcatc ggactcgtcg tcagcttcgt
     1321 cgcggtcttc atcggcggca tccggcttgt cctcggcttc gggtgcctgt aagggaggtg
     1381 tgcggtggac gaaaacctcg ggcggatgtt cgtgtggccg agggcgcgcg agtggtggga
     1441 ggcgctgcgg tgggtgctcc tcgtcttcac gctgctggtg ctcgtctcgc tcctcggctc
     1501 gttcgtctct tacgagcggg tcgtcgcaga ggggcacccg tggctgccgc gcgcgtacca
     1561 ctgcgcgggc tgcccgctgt gcggcatgac gcggagcttc tgcgcgctgt cgagcgggcg
     1621 ctggcacgag gccgtgcgct ggaaccgcgg cggccccgcg ctctacgccg ccgggtggct
     1681 ctggctgata accgcgaccg ccgtcgtcac ccggcgactt ctcggcggac gatagatgtg
     1741 ttgacgagcc gacgacgaat agtcgagagt cgcggctgct tgaaacaatg aagaggcggg
     1801 ccgtgaagca ttgcgacttc acggcccgcc tcgttcgtct gaagagtcga tgttctcagc
     1861 gacccctgcg gcgctcgaca tcgaggttgt tcgtcaccga gaagacgccg gacacgcccc
     1921 gcgcgcggat gtaggcgagg cggctgtcgc cctcgttcgc gacgacgcct tcgagcgtca
     1981 cgtggccgcg gttgacgacg atgtggatcg acgggttcgt cccgcgaccg tagcggaaga
     2041 gtgggctgtc cgcgccgaag agcgcgcggt aggtgcgcgc gcggatcgag tcgtcgaacc
     2101 cggagagagg cagcacctcg atccggttga cgacgcgcgc gacccccgcg agccgcgcca
     2161 cgcggcgctc cgcgtcactg cgcgtgctcg gtcgcacgac ctgaccgtac agcgtcaccg
     2221 tcccgccgtt gacctcgtaa gcgaggttgt cgaacacgcc gtagtaaggc agcgtgacca
     2281 gctcgcggcg aaccttcttc tccacctgcg cgcggtccac gctccggccc tgcgcgttcg
     2341 cgtccgcggc caacaccgcc gacgccgaca gggccagcgc cagcgcgaag aaaccgttgc
     2401 gtatcttttc catctctctc accccttgcg aaattacccg aaaatcactc tttttcatgc
     2461 gtcatttaga tgcccggcgc ggcgcctccg ttgtaggccg cgggcgggcg ccggggcgtc
     2521 cgccacgcac ttcgattctc cggcagccgc attttggtta tacttcccgc ggtcttcaga
     2581 aactacggaa acccggaacc tcgcgtgtga acgggcaggg aagcgacaac atcacgcggc
     2641 tgctgatcaa gctgacgaac ggcgacgccg ccgccgtcga tgcgctgctg ccgcacatct
     2701 acgacgagct gcgccgccac gccgccagct acctcaggcg cgagcgcccc gaccacacct
     2761 tgcagcccac cgcgctcgtc cacgaagcct acctgcggct cgtcgatcag acgcaggtgc
     2821 agtggcagaa ccgggcgcac ttcttcggcg tcgcggcgca gatgatgcgc cgccttctgg
     2881 tagaccacgc ccgcgcgcac aacgccgaga agcgcggcca cgatttccag aagctctcga
     2941 tagacgagaa catcgacaag gccgtcgagc gcagcgccga gctgctcgcc ctcgacgacg
     3001 cgctccgcga gctggccgag atcgacccgc agaaggcgcg gctcgtcgag ctgcgctact
     3061 tcggcggcct ctccgtcgag gagacggcgg aagtcctggg cgtcagcgcc cccaccgtca
     3121 aacgccactg gcgcgcgacc cgcgcgtggc tcttcggaag aatgcgacag ccgtgaaccg
     3181 tcaagagccg tgaaccgtaa accgtaaacc gtgatgggta ggaacagagt tcttctcatc
     3241 actcaacgct cattcactcg acacgttttt tcacttatca cggtttacgg ttcacggttc
     3301 acggctttcc tgtgatgacc cccgaacgct ggcggcaggt ggaagagatt ttccagacgg
     3361 cgctcgattt ggcgccgggc gagcgcgcgc ctttcttgga ggaggcgtgc ggcggggacg
     3421 ccgagctgcg gcgggatgtc gaggccatgc tcgcgcagta cgaagaggcg ggcgactttc
     3481 tggacgagcc gctggtcgag cagagcgggt tgcaggagct ggccgcgctg atgggcgagg
     3541 ccgacaagac cgaccctctg atcgggcagc gcgtcggcgc ctaccgcatc gagcgcgaga
     3601 tcgggcgcgg cgggatgggc gtcgtctacc tcgcgcagcg ggccgacggc gcgttccgca
     3661 tgcgcgccgc cgtcaaggtc atcaagcgcg gcatggacac cgacttcgtc ctgcgccgct
     3721 tccgccacga gcggcgcata ctggcttcgc tacagcacta caacatcgcg cgcatgttcg
     3781 acgcgggcgc gaccgaggcg gggctgccct acttcgtcat ggagtacatc gagggcgagc
     3841 cgctctacga ctactgcgac gcgcggcaac tgcctttgcg cgagcggctg cggctcttcg
     3901 cggaggtctg cgacgccgtc cactacgcgc accagaagca ggtcgtccac cgcgacatca
     3961 agcccccgaa catcctcgtc aacccttcgg gcgtgcccaa gctcttcgac ttcggcatcg
     4021 ccaagctgct caaccccgag ctgacgcccg acaccgcgcc gcagaccctg accgccatgc
     4081 gcctgatgac ggtcgagtac gccagcccag aacaggtgca gggtctgccg gtcacttccc
     4141 tgagcgacgt gtatagc
//
LOCUS       DBNGOH010000632         4555 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_460160, whole genome shotgun sequence.
ACCESSION   DBNGOH010000632 DBNGOH010000000
VERSION     DBNGOH010000632.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4555)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4555)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4555
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_460160"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..709)
                     /locus_tag="AC159P_12315"
     CDS             complement(<1..709)
                     /locus_tag="AC159P_12315"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003805856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase"
                     /protein_id="HIR3696275.1"
                     /translation="MTTSVESLTKAPVLEDYFGRSEAELHERIEAARRELGRRLLILG
                     HHYQRDDVIRHADYTGDSFKLSVLTGERPEAEFIVFCGVHFMAESADILKPGDQKVIL
                     PDLGAGCSMADMATSEQVEDAWEQLESIGVLGDFKIAPVTYMNSSAAIKAFCGRNGGV
                     VCTSSNAVPLFDKYLKEADKLFFFPDQHLGRNTGVKFGIPLDKMALWDPSKELGGNTE
                     EALRDARLILWKGHCSVH"
     gene            complement(959..2374)
                     /gene="fumC"
                     /locus_tag="AC159P_12320"
     CDS             complement(959..2374)
                     /gene="fumC"
                     /locus_tag="AC159P_12320"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014327027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045239 - tricarboxylic acid cycle
                     heteromeric enzyme complex [Evidence IEA];
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HIR3696276.1"
                     /translation="MTEEATGVGRAGATRVEKDSMGEIAVAGDRYWGAQTQRSLHHFD
                     IGFDVMPREMIRALGVLKKAAALVNEDLGKLPKDKAELIGRAADEVIEGKLDEHFPLR
                     VWQTGSGTQTNMNANEVISNRAIEMAGGVLGSKEPVHPNDHVNMSQSSNDTFPTAMHI
                     AAAEQMNALIPAVERLREAIEAKAEEFKDVVKIGRTHLQDATPLTVGQEFSGWASLVE
                     RDIERLKMVLPGLYDLAIGGTAVGTGLNSHPEFAERAAAKIAELTRLPFRAHPNKFAA
                     LSAHDEIVFASGALKTLAGSLMKIANDIRWLASGPRAGLGELVLPENEPGSSIMPGKV
                     NPTQSEAMTMVAVQVYGNDAAIGFAGSQGNFELNVFNPVMIHNLLHSIRLLKDACRGF
                     VEFCVEGIQLNRAKIDEHLRNSLMLVTALNQHIGYDNAAKIAKNAHHKGISLRESAVE
                     LGLLTADEFDAHVKPEEMTHP"
     gene            complement(2563..3618)
                     /locus_tag="AC159P_12325"
     CDS             complement(2563..3618)
                     /locus_tag="AC159P_12325"
                     /inference="COORDINATES: protein motif:HMM:NF024816.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3696277.1"
                     /translation="MRRDEAEELLEKLVAQTQDPRIFSAEVRQHIYETAEANPFLMEW
                     VVAQINEAQEPRAVLDELRHGEGDAAERVFTRSFNLPQVGDDGRAALLALSLFVPSAS
                     REALAEVAGFGDDQERLNEALRNLRALWLIKGLDENRRFTVEGLTRSLAGAHLSKDER
                     SGEFRQRFVAYFIRYAVAHENPTPEDYDAQEAEKDNLLSATDTAFTLEDWGSVVQMAY
                     ALANPVGGMLIVRGYWDEAPRVGNLALKAARASQAERLIMMMAHNVAVMYQRRGELNE
                     ARQLYGESLEINKRLGNQSGIASTLPQFGMLAEIEGDKAEAARLFREALSIFEKLRSP
                     NAEVARRSLERVEGKSS"
     gene            complement(3809..4405)
                     /locus_tag="AC159P_12330"
     CDS             complement(3809..4405)
                     /locus_tag="AC159P_12330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012165206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related family protein"
                     /protein_id="HIR3696278.1"
                     /translation="MDYPLQLSFKLLALSRQLSVRDARGQLLFYVKQKALKLKESVTV
                     FGDEGQTLPLYTIKADRVLDFNARYNFADTAGRHLGSVRRRGMRSIWKAHYEVYDEAD
                     RPDLDIKEENAWTKVFDSLLGEVPLVGMFTGYLFHPAYLVTRPTDAGAAPLLRLVKQP
                     AFFEGKFTLTKHTELSPTHETRALLALLMTILLERARG"
BASE COUNT          806 a         1532 c         1333 g          884 t
ORIGIN      
        1 cgtgcaccga gcagtggccc ttccacagaa tcaggcgcgc gtcgcgcaga gcttcctccg
       61 tgttgccgcc cagctccttc gacggatccc acagcgccat cttgtccaaa gggatgccga
      121 acttgacgcc cgtgttcctg ccaaggtgct ggtctgggaa gaagaagagc ttgtcggcct
      181 ccttcagata cttgtcgaag agcggcacgg cgttcgacga cgtgcagacc acgccgccgt
      241 tgcgcccgca gaacgccttg atcgcggcgc tggagttcat gtacgtcacc ggcgcgatct
      301 tgaaatcgcc gagcacgccg atggattcca actgctccca cgcgtcctcg acctgctcgg
      361 acgtagccat gtcggccatc gagcagccgg cgccgaggtc gggcaggatc accttctggt
      421 cgcccggctt caggatgtcg gcggattcgg ccatgaagtg gacgccgcag aagacgatga
      481 actccgcctc gggccgctcg cccgtcagca ccgagagctt gaacgagtcg cccgtgtagt
      541 ctgcgtgacg gatcacgtcg tcgcgctgat agtggtggcc gaggatcaag agtcggcggc
      601 caagctcgcg ccgcgccgcc tcgatccgct cgtgcagctc cgcctccgaa cgcccgaagt
      661 agtcttcgag caccggcgcc ttcgtcaaac tctccacgct cgtcgtcatc ctttgaaacc
      721 tcccgccgcg cctccaacgc acgactgccg cctctctgct ccgaggcgaa acgcgaaata
      781 taaaccccgc gcctgtgccg cgcaagcaac ccccgcacac aaccgccggg gcgattaatt
      841 agatgcgata cacaccccca aagttttaag gttaaaagcg gaaagggcga aaagggaagg
      901 agcaactcgc cgatcttcct tttcgccctt tcccctttta accttttccc ccttgccttc
      961 aagggtgcgt catctcttcc ggcttcacgt gcgcgtcgaa ctcgtctgcg gtgagcagtc
     1021 ccagctcgac cgccgactcg cgcaaggaga tgcccttgtg gtgcgcgttc ttggcgatct
     1081 tggcggcgtt gtcgtagccg atgtgctggt tgagcgcggt gacgagcatc agagaattac
     1141 ggaggtgctc gtcgatcttc gcgcggttga gctggatgcc ctcgacgcag aactcgacga
     1201 agccgcggca ggcgtccttc aagagccgta tcgagtggag caggttgtga atcatgacgg
     1261 ggttgaagac gttcagctcg aagttccctt gcgagcctgc gaagccgatg gccgcgtcgt
     1321 tgccgtaaac ctgcacggcg accatcgtca tcgcctcact ttgcgtcggg ttgaccttgc
     1381 ccggcatgat cgaagacccc ggctcgttct ccggcagcac cagctcgccc aggcccgccc
     1441 gcggccccga cgccagccag cggatgtcgt tcgcgatctt catcaaagac cccgccagcg
     1501 tcttcaaagc gccgctcgca aagacgattt catcatgcgc cgacagcgcc gcgaacttgt
     1561 tcgggtgcgc acggaacggc agcctcgtca gctccgctat cttcgccgcc gccctctccg
     1621 cgaactccgg gtgcgagttc agccccgtcc cgaccgctgt cccgccgatg gccagatcgt
     1681 agaggcccgg cagcaccatc ttcaaacgct cgatgtcgcg ctcgaccagc gaggcccacc
     1741 ccgaaaactc ctgccccacc gtcaaaggcg tcgcgtcctg caagtgcgtc cgcccgatct
     1801 tcaccacgtc cttgaactct tccgccttcg cctcgatggc ttcgcgcaaa cgctcgaccg
     1861 cagggatgag cgcgttcatc tgctccgccg ccgcgatgtg catcgccgtc gggaacgtgt
     1921 cgttcgaaga ctgcgacatg ttcacgtgat cgttcgggtg cacaggctcc ttgctcccca
     1981 acacgccccc cgccatctcg atcgcccggt tcgagatgac ctcgttcgca ttcatgttcg
     2041 tctgcgtccc gctccccgtc tgccacacac gcaacggaaa gtgctcgtcc aacttcccct
     2101 cgatcacctc atccgccgcc cgcccgatca actccgcttt atctttcggc agcttcccca
     2161 aatcctcatt caccagcgcc gccgcttttt taagcacgcc cagcgcccta atcatctccc
     2221 gcggcatcac atcgaatccg atgtcgaaat gatgcaacga acgctgcgtc tgcgcgcccc
     2281 aatacctatc ccccgcgacc gcgatctccc ccatcgaatc cttctccacc cgcgtcgcgc
     2341 ccgcacgccc cactcccgtc gcttcctctg tcatcgctta tcctcgtccc ctttcgccgc
     2401 gcgtcctact ctattaatac gaaaattccg tgttcgcgct ttatgattat tcgacaacgg
     2461 cggtgccccg aattctacac cagaacgccc gcctccccag ccccaccaaa accgaaggca
     2521 tccgccccaa atccacctac cgcaaatgct aaccgccaca ctttaagaag acttaccctc
     2581 aaccctctcc aaactccgac gcgcaacttc cgcattaggc gagcgcagct tctcaaaaat
     2641 actcagcgcc tcacgaaaca gccgcgccgc ctccgcttta tcgccttcga tttccgcaag
     2701 catgccaaac tggggcaggg tgctggcgat gccgctctgg ttgccgagcc gcttgttgat
     2761 ttccaagctc tctccgtaaa gctgccgcgc ttcattaagt tccccacggc gctgatacat
     2821 gacggccaca ttatgcgcca tcatcataat tagccgttca gcctgtgagg cacgcgctgc
     2881 tttgagtgcg aggttgccca ctcgcggcgc ctcatcccaa tagccacgca cgatcaacat
     2941 tccgcccaca ggatttgcca gagcgtatgc catctgcacg acgctccccc aatcttcgag
     3001 agtgaaggcc gtgtccgttg cgctcaacag attgtctttc tctgcttcct gcgcgtcgta
     3061 atcttccggc gtcggattct cgtgcgctac ggcgtagcgg ataaagtacg cgacgaaacg
     3121 ctggcggaac tcgccggagc gctcgtcttt cgataggtgc gctccggcga ggctgcgcgt
     3181 taagccttcg acggtgaagc ggcggttttc gtccagtcct ttaatcagcc acagcgcgcg
     3241 gaggttcctg agcgcttcgt tcaggcgctc ctggtcgtcg ccgaaacctg ctacctcggc
     3301 cagtgcctcg cgtgacgcgc tcggcacgaa gagcgagagg gcgagcagcg cggcccgccc
     3361 gtcgtcaccg acctgcggca ggttgaacga gcgtgtgaag acgcgctcgg cggcgtcgcc
     3421 ttcgccgtgc ctcaactcgt ccagcaccgc gcgcggctcc tgcgcctcgt tgatctgcgc
     3481 caccacccac tccatcagga acgggttggc ctccgccgtc tcgtagatat gctggcgcac
     3541 ctcggcggaa aaaatccgcg ggtcttgcgt ctgcgcgacc agcttctcca gcagctcctc
     3601 cgcctcgtcg cgccgcatgt cgagaatgac ttgcccacac cgaagctgcg acgacgccga
     3661 gcgcggcaac gatgagaccg ccgccgactt ccggacgcca ttcgatggga acctgctgcc
     3721 acatctctaa caaaagctgg cgtattgttt ccatgaggtt gtgcctcgct tttgaaaagg
     3781 ccggaacacc gcccgcgcgc gccgcgtgtc agccgcgggc gcgttcgagg aggatggtca
     3841 tgaggagggc gaggagggcg cgggtctcgt gggtggggga cagttccgtg tgtttggtga
     3901 gggtgaattt gccttcgaag aaggcgggtt gtttgacgag gcggaggagg ggggcggcgc
     3961 ctgcgtctgt ggggcgcgtg acgaggtagg cggggtggaa gaggtagccg gtgaacatgc
     4021 cgacgagggg gacttcgccg aggagggagt cgaagacttt ggtccaggcg ttctcttctt
     4081 tgatgtcgag gtcggggcgg tcggcctcgt cgtagacttc gtagtgggcc ttccagatgg
     4141 agcgcatgcc gcggcggcgc acggagccga ggtggcggcc cgcggtgtcg gcgaagttgt
     4201 agcgggcgtt gaagtcgagg acgcggtcgg ccttgatggt gtagagcggg agggtctggc
     4261 cctcgtcgcc gaagacggtg acggactctt tgagtttgag cgccttctgc ttgacgtaga
     4321 agaggagttg gccgcgcgcg tcgcggacgg agagctggcg cgagagcgcg agcagcttga
     4381 aggagagttg caaggggtag tccatcgtgt cctcgtttct cgcccgccgc cccgaaggga
     4441 ctcgcggcgg cgggcggctg gtaaagggtt cgtgaaatcg cgtcggaagc gacgccggga
     4501 gcaaagacaa tataagtaaa cgaggcgcgc gcggcaaaga ctttctcgcg gcgcg
//
LOCUS       DBNGOH010000633         4286 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3025919, whole genome shotgun sequence.
ACCESSION   DBNGOH010000633 DBNGOH010000000
VERSION     DBNGOH010000633.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4286)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4286)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4286
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3025919"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..858)
                     /locus_tag="AC159P_12335"
     CDS             complement(<1..858)
                     /locus_tag="AC159P_12335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014264112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HIR3696279.1"
                     /translation="MKQTWKAFIAAVATAGLLALAVAAPAQSTGDALARGFADPPDAA
                     SPRTWWHWTGSNVTVEGITKDLEWMKRAGIAGLQLADVNAGGGQSVEKKIVFGSPEWL
                     EAVRHAAAESDRLGLEMTIFSSAGWSETGGPWVKPEQAMKKLVWSETSLEGPSSFKGR
                     LPQPPSNNGPIRNLLTGGGRPGAAPPRDPTHYGDSAVVAYRAPPDEISAVDLKPKVTT
                     SAGSAGSVDAAALLDDDLNTTLAIPAPQGGGPAWVQFEFARPFRARAISLAGRGGIPV
                     GRVAASDDGS"
     gene            1133..2356
                     /locus_tag="AC159P_12340"
     CDS             1133..2356
                     /locus_tag="AC159P_12340"
                     /inference="COORDINATES: protein motif:HMM:NF024753.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB
                     family protein"
                     /protein_id="HIR3696280.1"
                     /translation="MAASSPDWPQWRGANRDGVSKESGLLKQWPAGGPRLLWQVNDIG
                     DGYSTPVVVGNRIYVMSNRGMENEFVQALSTQDGKPIWTTRVGNVGNPNQNPPYAKAR
                     STPTVDGNFIYALGSDGDLACLEAKTGKIRWQKNIRKEFGGVPGDWAYAESPLVDGDL
                     VVVTPGGAEATMAAVNKRTGATVWKSAIPGGDPAGYASAIAVQGGGRRQYVQLLPKGV
                     VGVDAKTGQFLWRYKECAKGPAQYFTPIGRGEHVYCGALGVGGTLVRLKPEAGGVAAE
                     QVYFTRGLPNGIGGAVLVGEHIYGTEAGGTLVAAEFATGKLNWKAESLGWASIAYADG
                     LLYVHGVNGDFALVEATPEGYREKGRFTPPAQPKKNQVGQFPEMAFAYPVIANGRLYI
                     RDLGTLWAYDIKASR"
     gene            2461..4119
                     /locus_tag="AC159P_12345"
     CDS             2461..4119
                     /locus_tag="AC159P_12345"
                     /inference="COORDINATES: protein motif:HMM:NF025690.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HIR3696281.1"
                     /translation="MAGLLCDHPLAELIHELSGGHLSGVLRVSRERAKGAVYFDEGRV
                     VGALTNLRAYRLTESLRRNGAVGAAKLDALLRPEMPDDAAGAALVAAGLLAPDELRRL
                     RERLASEVLRQLLQWEDGEWSFDARVRLDEGQHVRVEVGGLLVESARRLSPAFAAKSF
                     RDAGERLSLADATALAGGDANGGPQLQPSEGFVLSRFDAPLSLDELVAVSGLPDEETR
                     KAVYVLVLGGLLSRERPPRALPAAAAVAATTPRKTPPAAERPNATTATAETTKPEEEA
                     KPPAAEEATTEAAIAPKDPKDELAELFERAEAGSHYEVLGVGQLSKPDEIKRAYYALA
                     RRFHPDRFRQTVAEDERQRVELAFAKIAQAYDLLKDSAQRAAYDLKLSASRAPAPTAD
                     PAPPERKTETAQPRESDHAPRPDATADAPPAPPGKAGADLLRRAEELFQAGQAALGQQ
                     NYAAATKCLGEAVLLVPQQARYRAFFGRLLARDRTTRRQAEAELQAAISLDARNVSYR
                     LMLAELYRDVGLRRRAEAELEQALSIDRTSDAARRLLEELRKGG"
BASE COUNT          712 a         1449 c         1493 g          632 t
ORIGIN      
        1 cgacccgtcg tcactcgccg cgacgcggcc cacaggtatc ccgccgcgcc ccgcgagcga
       61 gatggcgcgc gccctgaacg gacgcgcgaa ctcgaactgc acccacgccg ggccgccgcc
      121 ctgcggggcg ggaatcgcga gagtcgtgtt gaggtcgtcg tccaatagcg ccgcggcatc
      181 gaccgagcca gccgatccgg ctgatgtagt cactttcggt ttcaagtcca ccgcactgat
      241 ttcgtcgggc ggggcgcggt aggcgacgac cgcgctgtcg ccgtagtggg tcgggtcgcg
      301 cggcggcgcg gcgccgggcc gaccgcctcc cgtcagcagg ttgcggatcg ggccgttgtt
      361 cgaaggcggt tgcggaagcc gacccttgaa gctgctcggc ccttccagcg aggtctcgct
      421 ccagacgagt ttcttcatcg cctgctcggg cttcacccag gggccgccgg tctcgctcca
      481 cccggcggag ctgaaaatcg tcatctccag ccccagccgg tcggactcgg cagccgcgtg
      541 gcggacggcc tccagccact ccggcgaccc gaagacgatc ttcttctcga cgctctgccc
      601 tccgcccgcg ttgacgtcgg ccagttgcag accggcgatg ccggccctct tcatccattc
      661 gaggtctttg gtaatccctt cgaccgtgac gttgctgccc gtccagtgcc accaagtcct
      721 cggactggcc gcgtcgggcg ggtctgcgaa accccgcgcg agcgcgtcgc ccgtggattg
      781 agcgggcgcg gcgaccgcca gcgccaacag gcccgccgtg gcgacagccg cgatgaacgc
      841 cttccatgtc tgtttcattt ttatcgggaa ccgcccggaa gagtctgacc ggcaaagttt
      901 atacacgaaa tgtatgcgaa cccgaacccg tgcaggctcg acgagaggcg gcggcgtgca
      961 gaccatgtgt gacgaaacat ctcgcgcgac tgatcgtatc aatgaagggc ggcgccaccc
     1021 agcaagcaat cccaacaacc ggaaggacaa gacagtgaag tcaagattac taacttccat
     1081 ggcagcgctc tgcctgtgcc tctcacccgc gttcgtcacg aggtctgccg acatggctgc
     1141 ttcgagtccc gattggcctc agtggcgcgg ggccaaccgc gacggggtgt cgaaggagag
     1201 cggcctcctc aaacaatggc cggcgggcgg cccccgactg ctctggcagg tgaacgacat
     1261 cggggacggc tattcgacgc cggtggtcgt cggcaaccgc atctacgtga tgagcaaccg
     1321 cggcatggag aacgagttcg ttcaggccct ctcgacgcag gacgggaaac ccatctggac
     1381 gacgcgcgtc ggcaacgtcg gcaacccgaa tcagaacccg ccttacgcga aggcgcgctc
     1441 caccccgacc gttgacggaa acttcatcta cgcgctcggc tcggacggcg acctcgcctg
     1501 cctcgaagcc aagacgggga agattcgctg gcagaagaat atccgcaaag agttcggcgg
     1561 tgtgccgggc gactgggcct acgccgagtc gccgctcgtt gacggcgacc tcgtcgtcgt
     1621 cacacccggc ggcgcggagg ccacgatggc ggctgtcaac aaacggacgg gcgcgacagt
     1681 ctggaagtcg gcgataccgg gcggcgaccc ggcgggttac gcatcggcca tcgccgtgca
     1741 gggcggcggg cgcaggcagt acgtacagct attacccaag ggcgtcgtcg gcgttgacgc
     1801 gaagaccggc cagttcctct ggcgctacaa ggagtgcgcc aaggggccgg cgcagtattt
     1861 cacacccatc gggcgcggcg agcatgtgta ctgcggagcg ctcggcgtcg gcggcacgct
     1921 cgtccgcctg aagccggaag ccggcggggt cgccgccgag caggtctatt tcacgcgcgg
     1981 gctgccgaac ggcatcggcg gggcggtgct ggtcggcgaa catatctacg gcacggaagc
     2041 cggggggacg ctggtcgcgg ctgagttcgc cacggggaag ttgaattgga aggccgagag
     2101 tctcggctgg gcgtcgatag cctacgccga cgggctttta tacgttcacg gcgtgaacgg
     2161 tgatttcgcg cttgtcgagg cgacccccga aggctaccgc gagaaggggc ggttcacccc
     2221 gcccgctcag ccgaagaaaa atcaggtggg gcagttcccg gagatggcgt tcgcgtaccc
     2281 ggtgatcgcc aacgggcgtc tctacatccg cgacctcggg acgttgtggg cctacgacat
     2341 caaggccagc cgctaggcgc tgacagcggc ggcggcgaat cgacggcgta gaagggtagt
     2401 cacgcccttc tacgcctttt gcgctttgcc ttttggctct cgccttctat catcatcggc
     2461 atggctgggc tgctgtgcga tcacccgctg gccgaactca ttcacgaact ttcgggcggg
     2521 cacttgtcgg gcgtgctgcg cgtgtcgcgc gagcgcgcga agggggctgt gtacttcgac
     2581 gagggccggg tcgtgggcgc gctcacgaac ctgcgcgcgt accgtctgac cgagtcgttg
     2641 cggcgcaacg gggcggtggg cgcggccaag ctcgacgcgc tgctgaggcc ggagatgccg
     2701 gacgacgcgg cgggcgcggc gctcgtcgcg gcggggctgc tcgcgccgga tgagctgcgg
     2761 cgcctgcgcg agcggctggc ttccgaggtc ttgcgccaac tgctgcaatg ggaggacggc
     2821 gagtggagtt tcgacgcgcg cgtgcgtctg gacgagggcc agcacgtgcg cgtcgaggtg
     2881 ggcgggctgc tcgtcgagag cgcgcggcgg ctgtcgccgg ccttcgccgc gaaaagtttt
     2941 cgcgacgcgg gcgagcggct gtcgctcgcg gacgcgacgg cgctcgcggg cggcgacgcg
     3001 aacggcggcc cgcagctcca gccgtcggaa gggttcgtgc tgtcgcgctt cgacgcgccg
     3061 ctcagtctcg acgagctggt cgccgtcagc gggctgcccg acgaagagac gcgcaaagcc
     3121 gtctacgtgc tggtgctcgg cgggctgctc agccgcgagc gaccgccccg cgcgttgccc
     3181 gccgcggcgg cggttgccgc gaccacgccg aggaaaacgc cgcccgccgc cgaacgcccg
     3241 aacgcgacga ccgcgacggc ggagacgacg aaaccggagg aggaggcgaa gccgcccgcg
     3301 gcggaggagg cgaccaccga ggcggccatc gcgccgaaag acccgaagga cgaactcgcg
     3361 gagttgttcg agcgcgccga agcgggttcg cactacgaag tgctcggcgt cggtcaactc
     3421 tcgaagccgg acgagatcaa gcgcgcctac tacgcgctcg cgcgccgctt ccacccagac
     3481 cgcttccggc agaccgtggc cgaggacgag cgccagcgcg tcgaactcgc cttcgccaag
     3541 atcgcgcagg catacgacct gctcaaggac tcggcgcagc gcgccgccta cgacctcaag
     3601 ctctccgcgt cgcgcgcgcc ggcgccgacc gccgacccgg cgccgcccga gcgcaaaacc
     3661 gagacggcgc agccgcgcga aagcgaccac gcgccgcgac cggacgcgac cgccgacgcg
     3721 ccgcccgcgc cgcccggaaa agccggcgcc gacctgctgc gccgcgccga agagttgttc
     3781 caggcggggc aggccgcgct cggccagcag aactacgccg ccgcgacgaa gtgcctcggc
     3841 gaggcggtgc tgctcgtccc gcagcaggcg cgctaccgcg ccttcttcgg gcgcctgctc
     3901 gcgcgcgaca ggacgacgcg gcggcaggcc gaggccgagc tacaggccgc catctcgctc
     3961 gacgcgcgca acgtctcgta ccggctcatg ctcgccgagc tgtaccgcga tgtcggcctg
     4021 cgccgccgcg ccgaggcgga gcttgagcag gcgctctcga tcgaccgcac gagcgacgcg
     4081 gcccgccgcc tgctcgaaga gttgcgcaag ggcggttgaa cataacagcg tggaagacac
     4141 tttggatttc ggatttcgga tttcggattt cggattgatg tcgattaagg gatgacgatt
     4201 gatgacagcg ccgttcccgt ccatcgtcaa cggtgggagc cggaacatcg gagaaggcat
     4261 caggctcaaa caacacgcca atccaa
//
LOCUS       DBNGOH010000634        12737 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4675385, whole genome shotgun sequence.
ACCESSION   DBNGOH010000634 DBNGOH010000000
VERSION     DBNGOH010000634.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 12737)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12737)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12737
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4675385"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            71..1582
                     /locus_tag="AC159P_12350"
     CDS             71..1582
                     /locus_tag="AC159P_12350"
                     /inference="COORDINATES: protein motif:HMM:NF017044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HIR3696282.1"
                     /translation="MKISRTFSSLLLALVLLADPASLLAQTGQQGVPAHPRELKYTTL
                     TYTPPKRDRYRHVLSNGVVVYAVEDHDLPLVNISTLVRTGSYLDPAGKEGLAALTGSQ
                     MRAGGTTSKTAEQFDEAADFLAAAISSFISGTQGSASVNMLTKDVDAGLALFFDMLRN
                     PAFQEDRLKLAKSQSLQAMERRNDRTDAIENREWTRLVRGDAHFTAKDSTKASIESIT
                     REDLAAFHKRYYQPANFIFAVSGDFETKQLISKLEAAMKDWPSTKMAVPDVPKPDFTP
                     VAGVYAVHKADVNQGRVTVGHLGSMRDNPDAFALSIMNDILGGGGFTSRITSRVRSDE
                     GLAYSAGSSYGLGTYYPGVFQASFQSKSPTTAQALDIVLEEINRIRTTKVSAEELETA
                     KNYAVEIFPRFFATAGQIAGTFAQDEFTKRQADYWDKYRERVRAVTADDVQRVAQKYL
                     APDKLVVLVVGNIDDITKGNPDRPQHSLVKVGGGTVRRIPLPDPLTLVYPATQ"
     gene            1755..2282
                     /locus_tag="AC159P_12355"
     CDS             1755..2282
                     /locus_tag="AC159P_12355"
                     /inference="COORDINATES: protein motif:HMM:NF024822.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4112 domain-containing protein"
                     /protein_id="HIR3696283.1"
                     /translation="MAMSKQLAKAEGRGVGREVRAKEDIERSLDQLSRYMDGLFRIPG
                     TGWRVGLDALVGLVPGVGDFATTAVSFYILAAGVRYRVPKVTLLRMALNIAVDYVVGS
                     VPVVGDILDAAWKSNQKNVALLRERATVGASEAREASLSDWLFVGLIMLVLLVLLLGS
                     IGFSLWLLSRIFGSF"
     gene            complement(2358..2849)
                     /locus_tag="AC159P_12360"
     CDS             complement(2358..2849)
                     /locus_tag="AC159P_12360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007202759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HIR3696284.1"
                     /translation="MPMRIGTEMPPLDGATEWFNATGAHAEVEAKGRPTLVHFWAVSC
                     GICKENMPRVAEWRDGRAAEGLRVVAVHMPRYPADTDAEAVRGAVATYNITEPCAVDN
                     EHRLRDAFKNDQGFVPAYYLFDTEGKLRCFAAGERGLAMLGPALERVLAAATKEAASA
                     GLS"
     gene            complement(2947..3492)
                     /locus_tag="AC159P_12365"
     CDS             complement(2947..3492)
                     /locus_tag="AC159P_12365"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016482398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HIR3696285.1"
                     /translation="MAEALVGKPAPDFDMPSTKNIEKLNENVKLSDYAGKWVVLLFYP
                     LDFTFVCPTELTTFSDRVEDFDGIGAEVIGVSTDSVHSHRAWLRTPREQNGVEGLKYP
                     LASDITKDVARSYGVLIEDKGIALRGLFVIDPEGILRYAVVHDLNVGRSAEETIRVIQ
                     ALQTGGLCQAEWRPGQETLKV"
     gene            complement(3597..4016)
                     /locus_tag="AC159P_12370"
     CDS             complement(3597..4016)
                     /locus_tag="AC159P_12370"
                     /inference="COORDINATES: protein motif:HMM:NF013629.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HIR3696286.1"
                     /translation="MTSGKRDQGGGGRASLTPQRRAVLQVVSESAEHLTAGEIYEAAR
                     RVLPTISFATVYNSLKYLKEAGLLGEISFGKASSRYDRETARHDHAVCTCCGKLVDFD
                     LEETPRLARAAARRSRFKAVSMHLTLVGVCPDCQKKD"
     gene            complement(4170..5249)
                     /locus_tag="AC159P_12375"
     CDS             complement(4170..5249)
                     /locus_tag="AC159P_12375"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013530.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR3696287.1"
                     /translation="MTFDETVFLTGFPGFIAERLVERLARGGARFLLLVQPAFLERAR
                     EDAARIARETGAAPENFRVVEGDITQNDLGLAAGDLEAARRETTVVFHLAAVYDLAVE
                     HEPAMRVNVEGTRRVNRFARGVRDLKRYHYVSTCYVAGLRTGLIRESELRHEAGFRNF
                     YEETKYLAELEVDELKRELPVTIHRPSVVCGDSRTGETAKYDGVYYLINYLRMRPDLL
                     SLANIGNREVRLNVVPVDFVVEAMAALAADDRALGATVQLADPAPLTTHELFDTIART
                     LAGRPSRLTVPATFVQASLNLRFSEMLTGLPRAGAPYFFIKQTYDTTRAQALLEPRGV
                     RCPRFPDYVEALIEYVERKEEGRKG"
     gene            complement(5294..6019)
                     /locus_tag="AC159P_12380"
     CDS             complement(5294..6019)
                     /locus_tag="AC159P_12380"
                     /inference="COORDINATES: protein motif:HMM:NF047539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XAC2610-related protein"
                     /protein_id="HIR3696288.1"
                     /translation="MKRLVLLTLFLAPFAHVSFAQGEEFRIERASKKYDLAVRVEECG
                     GPEQRDDPDTCGGPARVSFYRKGAKTPFQTLRLPNVEIYRETLAHNPQTSAKPRRLYA
                     EEYGFVFDDFDFDGAEDLAICNGRNSGYGGPSYSVFLFDAKTRKFVENRRLSALAEGA
                     YLGLFFPDKKKKQLVAHSKSGCCYHETEKYKVVNNRPVLVEKITEQATGRDDGAFDVV
                     TTTSRRVGHRWITRVKREVSKGY"
     gene            complement(6044..7588)
                     /locus_tag="AC159P_12385"
     CDS             complement(6044..7588)
                     /locus_tag="AC159P_12385"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF002073.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HIR3696289.1"
                     /translation="MNKPNSLSVAIGGDTLSPDTTAGAIAVGVFSDEPRVACAEVLDD
                     DLRRLCEQVAAGGEFEGEEGTALLIHAAGANGASAAGSVGTRRVLLVGLGARADFDGA
                     VLRRAASVAVRSARDAHARTLHFVMPPAGETTGGASAARALAEGAHLGLYDGDLYQNH
                     EEERPALERLSIVAPRADGDASADEVERGRIIAESVNWARTLADEPGASLPPREFARR
                     AAEMAAEFGLRVETLDAAEIRARGMGGLAGVGQGSENEPALIVLRYVPESAPEDDELY
                     AFVGKGITFDTGGISLKKPQGMEEMKSDMAGGAAALGALRAVAQLKPRRRVVAVVPSA
                     ENMPSGRALKPGDIVRTLAGYTIEVVDTDAEGRLVLVDGIAYAKQLGATRIVDLATLT
                     GSIIIALGTHRTGLFSNDDAWAEEVQRASERAGEPVWRMPVGDDYKKRIESPIADFKN
                     YGGRPDATAAALLLSKFAADTPWVHLDIAGTAWHDDPQPHAPKGSTGAGVRTLVELVC
                     GRAADV"
     gene            7764..9347
                     /locus_tag="AC159P_12390"
     CDS             7764..9347
                     /locus_tag="AC159P_12390"
                     /EC_number="3.1.-.-"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF012376.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HIR3696290.1"
                     /translation="MTHIRTHALRAALAALLVLAVSARPAPAQVAPQDCKVRVTLLQV
                     NDVYQFLPVERGAEGGLARVSTLRKEIMRGSPNTLFLLSGDTISPSVESITYKGRQMI
                     DAWNNVQLDYSVFGNHEFDFGPDVLRERVAESKFKWLGANVIDRKTGKLFADTPLFDI
                     REFDGVKVGIFGIVLPESKTTSSPGPDVDFLDSCETANRVIPRIRAAGAQTIVALTHL
                     SMAEDKQLARCAPGIDVIIGGHEHTLLQSSSAGVPIFKMTADAREMGRIDLNIDRKTG
                     KVESVDWQIIPVGRSVAEDPAFAAVTEKYAAKLTELASVVGRTEVALEARSEPSRTVE
                     TNAADFIADAFRRAASADVALLNGGSIRADTVISAGDLTLRDVLSILPFGNDLVKIEV
                     TGDVLRQALEHGVSRTGPGSEPGRFPQVSGLRYSFDATLTPGARVKTVTVGDQPLDAK
                     RKYTLVTTRYVAEGGDQYAMLKGHPNVLKEKLIDNEVLRRAIADAKVIAPRTDGRLQR
                     LDKPADEKPCKVAAAAGGK"
     gene            9416..9919
                     /locus_tag="AC159P_12395"
     CDS             9416..9919
                     /locus_tag="AC159P_12395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3696291.1"
                     /translation="MFDEFDDAPPSAKTRAIELFESAYRAQVEKDYDAAIELYRRSIE
                     TYPTAEAHTFLGWVYSFQERYQDAIDECLEAIRVDASLGNPYNDIGSYLIAQGDLWSC
                     VRWLKLALHAPRYEARAFPHFNLARVYEQRHRPLEAARHYGLALEQQPDFSQATRALR
                     RLQARLN"
     gene            complement(10014..10436)
                     /locus_tag="AC159P_12400"
     CDS             complement(10014..10436)
                     /locus_tag="AC159P_12400"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016646522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HIR3696292.1"
                     /translation="MNSYGNPAIRITLLPRDTNPQGTIFGGIILSYIDIAGAVEAHRR
                     TGIERFVTVAMREVVFHEPVYVGDLVSFYAETERIGTTSITVRVVVEAERYGGTSERI
                     KVTEAEVVYVAVDANRRKMRIDRDKVKDLPSIEQHETS"
     gene            10817..>12737
                     /locus_tag="AC159P_12405"
     CDS             10817..>12737
                     /locus_tag="AC159P_12405"
                     /inference="COORDINATES: protein motif:HMM:NF013592.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HIR3696293.1"
                     /translation="MKRLLTRSALLLALLTLSNAAPRAAAQDRRPQRRGAQAARPTPT
                     PTPQPTPAATTQLREAPLPPGGRARFDVNHYRIEAELLPGQHQLNAVGDVTFTPRDNT
                     RSVIFELNGSLKVEGIERNGKPIINFVQDAAGYDTTGPNVRVDLGEVVAAGQPVTLRF
                     KWSGSLVTPEGGPLAAKRLAFVGTDLSYLMYAARWFPFHDYAADRATSEMTITVPQGY
                     TVAGASDEAVTPRPAGAKVAYRFVHKQPALVGNFAAARYVSRTLRMGGFEMTFHVLPG
                     SERFVEAYAQLMGDAVEFYTHKYGQPASGARLVVAQIDDDSLDAYAAPGMLFLSARYF
                     SGAGGTQQQAMDERVQRELAYQWWGHTVGLKTFDDAWLSQGLAEWSAFALREQKLQGA
                     ALESAQRDMLERALMFEQSASIARAPSTLDDQSAAYQAVVFYKGAMVFRMLRETLGPQ
                     KFDELMRRFYNENRGKNASIDDFERVATTVHSDNLRYFFARWVEGTGVPEFTSDYQII
                     RTRGGKFRARGTVKQNTENLKLPIDVMLRAEGDDASATVYVADRSEDFDFESAGKPLE
                     IIIDPNNKVLRTSEELRVSVVARRGLELFREGQYLEAQRQLEEALKLDKSNSWVYYNL
                     GLIFLEQRNYQQALDN"
BASE COUNT         2091 a         4442 c         4108 g         2096 t
ORIGIN      
        1 agggcataca ggttgtgaag aagatgctgg aggatcgcct caagcagctc gaaggaggtg
       61 tcaagtagtc atgaaaatca gcagaacctt ttcgtcgctg ctcctcgcac tcgtcctgct
      121 cgccgacccg gcctcgctcc tcgcgcagac ggggcagcag ggcgtcccgg cgcacccgcg
      181 cgaattgaag tacacgacgc tcacctacac tccccccaag cgcgaccgct accgccacgt
      241 gctctcgaac ggcgtggtcg tctacgcggt cgaggaccac gacctcccgc tcgtcaacat
      301 ctcgacgctc gtccggacgg ggtcgtacct cgacccggcg ggcaaggaag gtctcgccgc
      361 tttgaccggc agccagatgc gcgccggggg caccacctcg aagaccgccg agcagttcga
      421 cgaggccgcc gacttcctcg ccgccgccat cagcagcttc atctccggca cgcaagggag
      481 tgcgagcgtc aacatgctga cgaaggacgt ggacgccggc ctcgcgctct tcttcgacat
      541 gctccgcaac cccgcttttc aggaagaccg tctgaagctc gccaagagcc agtcgcttca
      601 ggcgatggag cggcgcaacg accgcaccga cgccatcgag aaccgcgagt ggacgcggct
      661 cgtgcgcggc gacgcgcact tcaccgcgaa ggactcgacg aaagcctcca tcgaatccat
      721 cacgcgcgaa gacctcgccg ccttccacaa gcgctactac cagcccgcca acttcatctt
      781 cgccgtctcg ggcgacttcg agaccaaaca gttgatctcg aagctcgaag cggcgatgaa
      841 ggactggccc tcgacgaaga tggccgtgcc cgacgtgccg aagcccgact tcacgccggt
      901 cgcgggcgtc tacgccgtgc acaaggcgga cgtgaaccag ggccgcgtca cggtcggcca
      961 cctcggctcg atgcgcgaca acccggacgc cttcgccctc tcgatcatga acgacatcct
     1021 cggcggcggc ggcttcacct cgcgcatcac ctcgcgcgtc cgctccgacg aggggttggc
     1081 ctacagcgcc ggctcgtcct acggcctcgg cacctactac cccggcgtct tccaggccag
     1141 cttccagtcg aagtcgccga ccaccgcgca ggcgctcgac atcgtgctcg aagagatcaa
     1201 ccgcatccgc accaccaagg tctcagccga agagcttgag acggcgaaga actacgccgt
     1261 cgaaatcttc ccccgcttct tcgccaccgc cgggcagatc gccggcacct tcgcgcagga
     1321 cgagttcacg aagcggcagg ctgactactg ggacaagtac cgcgagcgcg tgcgcgccgt
     1381 caccgccgac gacgtgcagc gcgtcgcgca gaagtacctg gcgcccgaca agctcgtcgt
     1441 cctcgtcgtc ggcaacatcg acgacatcac caagggcaac cccgaccgcc cgcagcactc
     1501 gctcgtcaaa gtcggcggcg gcaccgtcag acgcatcccg ctccccgacc cgctgacgct
     1561 cgtctacccg gcgacgcagt aagccgagcg cgcgtcgaac ctcggaaccc gcgaaggccg
     1621 cgcgacgttt gtatacgtcg cgcggccttt ctctcccgcc gctcactccc ctaagatgga
     1681 cgcgagcatc cggtgctttg caagttcccg cgtacgggca cggccacgcg gcggcggacg
     1741 aggaggagta ggtcatggca atgagtaagc agttggcgaa agccgagggg cgcggcgtgg
     1801 ggcgcgaggt gcgcgcgaag gaagacatcg agcggagcct cgaccagctc agccgctaca
     1861 tggacgggct gttccgcatc cccggcacgg gctggcgggt cggcctcgac gcgctcgtcg
     1921 ggctggtgcc gggcgtcggc gacttcgcca cgaccgccgt ctccttctac atcctcgccg
     1981 cgggcgtccg ctaccgcgtc cccaaagtga cgctgctgcg gatggcgctc aacatcgccg
     2041 tcgattacgt ggtcgggtcg gtgccggtcg tcggcgacat cctcgacgcg gcgtggaagt
     2101 cgaaccagaa gaacgtcgcg ctgctgcgcg agcgcgccac ggtcggcgcg tccgaagcgc
     2161 gcgaggcgag cctctcggac tggctcttcg tcggcctcat catgctcgtc ctcctcgtcc
     2221 tcctgctcgg ctccatcggc ttcagcctgt ggctgctgag ccggattttc ggtagcttct
     2281 gacgcgcgcg atttcgaaga aaacagaaag cgcgcccttc atcatgaagg gcgcgctttc
     2341 tgttttccga catgatatta gctcagccct gccgaggccg cctctttcgt cgccgccgcg
     2401 agcacgcgtt cgagcgccgg gccgagcatg gcgaggccgc gctcgcccgc cgcgaagcag
     2461 cggagcttcc cttcggtgtc gaacaggtag taagcgggga cgaacccctg atcgttcttg
     2521 aaggcgtcgc gcaagcgatg ctcgttgtcc acggcgcacg gctccgtgat gttgtaggtg
     2581 gcgacggcgc cgcgcaccgc ctccgcgtcc gtgtccgccg ggtagcgcgg catgtgcacg
     2641 gcgaccacgc gcaagccttc ggcggcgcgc ccgtcgcgcc actccgccac gcgcggcatg
     2701 ttctccttgc agatgccgca gctcaccgcc cagaagtgca ccagcgtcgg gcgccccttc
     2761 gcctccacct ctgcgtgcgc gcccgtcgcg ttgaaccact ccgtcgcgcc gtccagcggc
     2821 ggcatctccg tcccgatcct catcggcata gcttcacctc aacaaacgta aatcgtgaac
     2881 cgtaaaccgt gataagagaa aaccgcttct tacttgtcac ggtttacggt tcacggttca
     2941 cggtcttcag actttgagcg tctcctggcc cggacgccac tcggcctggc agaggccgcc
     3001 ggtctggagc gcctggatga cgcggatggt ctcctccgcc gagcgcccga cgttcaggtc
     3061 gtgcacgacc gcgtagcgca ggatgccctc ggggtcgatc acgaacaggc cgcgcagcgc
     3121 gatgcccttg tcctcgatca gcacgccgta gctgcgcgcg acatccttgg tgatgtccga
     3181 agcgagcggg tacttcaagc cctcgacgcc gttctgctcg cgcggcgtcc gcagccacgc
     3241 gcggtgcgag tgtaccgagt cggtcgagac gccgatcacc tcggccccga tgccgtcgaa
     3301 gtcctcgacg cggtcgctga acgtggtcag ctccgtcggg cagacgaagg tgaagtcgag
     3361 cgggtagaac aagaggacaa cccacttgcc cgcgtaatcc gagagcttca cgttctcgtt
     3421 cagcttctcg atgttcttcg ttgacggcat gtcgaaatcc ggtgccggct tgccgaccaa
     3481 tgcttctgcc atgatcttga tgattcctcc gttgaagttt tagagcttat tttcgaacac
     3541 gcgacgcttc atcgaacctt aacgccgcgc gcgctaacga ccgacgtatc aaaccctcaa
     3601 tccttcttct gacagtccgg gcacacgccc acgagcgtca ggtgcatcga caccgccttg
     3661 aagcgcgagc ggcgcgcggc ggcgcgcgcg agccgcggcg tctcttccag atcgaagtcc
     3721 acgagtttgc cgcagcacgt gcagacggcg tggtcgtgcc gcgccgtctc gcggtcgtag
     3781 cggctcgacg ccttgccgaa gctgatctcg ccgagcagcc cggcctcctt caggtacttc
     3841 agcgagttgt agacggtggc gaagctgatc gtcggcagca cgcgccgcgc cgcctcgtaa
     3901 atctcgcccg ccgtcaggtg ctccgccgac tcgctcacca cctggagcac ggcccgccgc
     3961 tgcggcgtca aagacgcacg ccccccgcca ccctgatctc tcttgcccga agtcatgatt
     4021 gttttaagct ggaattagaa taattctaat tcgtggactt gtcaactgct caggatgagg
     4081 gaaaaggtta agaggagaag aggggaaaag gggaaaaggg aggcagagga cagcgggcct
     4141 cttatcctcc ctcttcgccc tttccccttt taaccttttc gcccttcttc cttgcgttcg
     4201 acgtattcga tgagcgcctc gacgtagtcg gggaagcgcg ggcagcggac gccgcggggt
     4261 tcgagcaagg cttgggcgcg ggtcgtgtcg taggtctgct tgatgaagaa gtagggggcg
     4321 ccggcgcgcg gcaggcccgt cagcatttcg gagaagcgca ggttgagcga agcctgcacg
     4381 aaggtcgcgg ggacggtgag gcgcgagggg cgcccggcga gcgtgcgggc gatagtgtcg
     4441 aacagctcgt gcgtggtgag cggcgcgggg tctgcgagct ggacggtcgc gccgagcgcg
     4501 cggtcgtccg ccgcgagcgc ggccattgcc tcgacgacga agtccacggg cacgacgttg
     4561 aggcgcacct cgcggttgcc gatgttggcg agcgagagca ggtcggggcg catccgcagg
     4621 tagttgatga ggtagtagac gccgtcgtac ttcgccgtct cgcccgtccg cgagtcaccg
     4681 cacaccaccg acgggcggtg gatcgtcacc ggcagctccc gcttcaactc gtcaacttcc
     4741 agctcggcga ggtacttcgt ctcctcgtaa aaattgcgga agcccgcctc gtgccgcagc
     4801 tcgctttcgc ggatcagccc cgtgcgcagc cccgcgacgt agcacgtcga gacgtagtga
     4861 taccgtttca aatcccgcac gccgcgcgcg aaccggttga cgcggcgcgt gccttcgacg
     4921 ttgacgcgca tcgccggctc gtgctcgacc gcgaggtcgt aaacggctgc gaggtgaaac
     4981 acgacggtcg tctcgcgccg cgccgcctcc agatcgcccg ccgccagtcc caaatcattc
     5041 tgcgtgatgt cgccttcgac gacgcggaaa ttttcgggcg cggcgcccgt ctcgcgcgcg
     5101 atgcgcgccg cgtcctcccg cgcgcgctca aggaacgcgg gctgcacgag cagcaggaag
     5161 cgcgcgcccc cgcgcgccag ccgctcgacc agccgctcgg cgatgaagcc ggggaagccg
     5221 gtcaggaata cggtctcatc gaacgtcatg cgttactgct caagccccct tgtcagaact
     5281 tcgactccga aagtcagtat cctttgctga cttctcgctt gacccgcgtg atccacctgt
     5341 ggcccacgcg tctgctggtc gtcgtcacca cgtcgaacgc gccgtcgtcg cggcccgtgg
     5401 cctgctcggt tatcttctcc accagcacgg gcctgttgtt cacgaccttg tacttctcgg
     5461 tctcgtggta gcagcagccc gatttcgagt gcgcgacgag ctgttttttc ttcttgtccg
     5521 ggaagaagag gccgaggtaa gcgccttcgg cgagtgccga aagtcgcctg ttctcgacga
     5581 acttgcgagt cttcgcgtcg aagagaaaga cgctgtacga aggcccgccg tagccgctgt
     5641 ttctcccgtt gcagatggcc aagtcctccg caccgtcgaa gtcgaagtcg tcgaagacga
     5701 acccgtactc ctcggcgtag agccggcgcg gcttcgcgct ggtctgcggg ttgtgggcca
     5761 gcgtctctct gtagatttcg acgttgggca ggcgcagagt ttggaacggc gtcttcgcgc
     5821 ctttccggta gaagctgacg cgcgccgggc cgccgcaagt gtcggggtcg tcgcgctgct
     5881 ccggcccgcc gcactcttcg acccggacgg cgagatcgta cttcttcgaa gcgcgctcga
     5941 tcctgaactc ctcgccctgt gcgaacgaca cgtgagcgaa gggggcgagg aacagcgtga
     6001 gcagaaccaa ccgcttcatg gcttcagtct ctcttgcgga gccttaaacg tccgcggcgc
     6061 gaccgcacac cagttcgacg agcgtgcgca cgcccgcgcc ggtcgagcct ttgggcgcgt
     6121 ggggctgtgg gtcgtcgtgc caggcggtgc cggcgatgtc gagatgcacc cagggcgtgt
     6181 cggcggcgaa cttgctgagc aggagcgcag cggcggtggc gtcggggcgg cctccgtagt
     6241 tcttgaagtc ggcgatgggg ctttcgatgc gcttcttgta gtcgtcgccg acgggcatgc
     6301 gccagacggg ttcgcccgcg cgctcggagg cacgttgcac ttcctcggcc cacgcgtcgt
     6361 cgttgctgaa gaggccggtg cggtgcgtgc cgagggcgat gatgatcgag cctgtgagcg
     6421 tggccagatc gacgatgcgt gtggcgccga gctgcttcgc gtaggcgatg ccgtccacga
     6481 ggacgaggcg gccttcggcg tccgtgtcca cgacttcgat ggtgtagccg gcgagcgtgc
     6541 gaacgatgtc gcccggtttc agcgcgcggc ccgaaggcat gttctcggcg gaagggacga
     6601 cggccacgac gcggcgtcgc ggtttgagct gcgcgacggc gcgcagcgcg ccgagcgcgg
     6661 cggcgccgcc cgccatgtcg gacttcatct cctccatgcc ctgcggcttc ttgagcgaga
     6721 tgccgcccgt gtcgaacgtg atgcctttgc cgacgaaggc gtacagctcg tcgtcctccg
     6781 gtgcgctctc cggcacgtaa cgcaggacga tcagcgccgg ctcgttctcc gacccctgcc
     6841 cgactccggc gaggccgccc atgccgcgcg cgcgaatctc cgcggcgtcc agagtctcta
     6901 cccgcaggcc gaactccgcg gccatctcgg cggcgcggcg cgcgaactcg cgcggcggga
     6961 gcgacgcgcc cggctcgtcc gcgagcgtgc gcgcccagtt cacggactcc gcgatgatgc
     7021 gcccgcgttc gacctcgtcg gcgctcgcgt cgccgtcggc gcgcggcgcg acgatggaga
     7081 ggcgttcgag cgcgggccgc tcctcttcgt gattctgata gaggtcgccg tcgtagagtc
     7141 cgaggtgcgc gccttccgcg agtgcgcgcg ccgccgacgc gccgcccgtc gtctcgcccg
     7201 cgggcggcat gacgaagtgg agcgtgcgag cgtgcgcatc gcgcgcggag cgcaccgcga
     7261 ccgaagccgc gcgacggagc accgcgccgt cgaagtcagc gcgcgcgccg aggccgacga
     7321 gcaggacgcg gcgcgtgccg acgctgcccg ccgccgacgc gccgttcgcg cctgccgcgt
     7381 ggatgagcag cgccgtgcct tcctcgccct cgaactcgcc gcccgccgcg acctgttcgc
     7441 agagtcgccg caagtcatcg tcgagcactt cggcgcaggc cacgcgtggc tcgtcggaga
     7501 agacgccgac ggcgattgcg cccgcggtcg tgtcgggaga aagagtgtcg ccgccgatgg
     7561 ctactgaaag gctgttcggt ttgttcatgg ggggtgattt aaagggatgt ttggtgcgtt
     7621 tcgttcatgg cgagtataat tcgaactctc gatttggcct aacttcgaag gcggacgcgg
     7681 gtcgcgtgct cttctcgtcg gcgacgcccg acgcgcacgc gaccggcaca ctcccgacac
     7741 caccaaggag gctatcttta cagatgactc acatccgcac gcacgccctg cgcgccgcgc
     7801 tcgccgcgct gctcgtgctc gccgtttccg cgcggcccgc gcccgcgcaa gtcgccccgc
     7861 aggactgcaa ggttcgcgtc acgctgcttc aggtcaacga cgtgtaccag ttcctgcccg
     7921 tcgagcgcgg cgccgaaggg ggcctcgcgc gcgtctcgac gctgcgcaag gagatcatgc
     7981 gcggctcgcc gaacacgctc ttcctcttgt cgggcgacac catctcgccg tcggtcgagt
     8041 cgatcaccta caaggggcgg cagatgatcg acgcgtggaa caacgtccag ctcgactact
     8101 cggtcttcgg caaccacgag ttcgacttcg gccccgacgt gctgcgcgag cgcgtcgccg
     8161 agtcgaagtt caagtggctc ggcgcgaacg tcatcgacag aaagaccggc aagctgttcg
     8221 ccgatacgcc gctcttcgac atccgcgagt tcgacggcgt gaaggtcggc atcttcggca
     8281 tcgtgctccc cgagtcgaag accacctcca gccccggccc cgacgttgac ttcctcgact
     8341 cctgcgagac cgccaaccgc gtcatcccgc ggatccgcgc ggcgggcgcg cagacaatcg
     8401 tcgcgctcac gcacctctcg atggccgaag acaagcagct cgcgcgttgc gcgcccggca
     8461 tcgatgtcat catcggcggg cacgagcaca cgctgctgca atcctcttcg gcgggcgtgc
     8521 ccatcttcaa gatgacggcg gacgcgcgcg agatggggcg catcgacctc aacatcgacc
     8581 ggaagaccgg caaggtcgag agcgtcgatt ggcagatcat ccccgtcggg cgctcggtcg
     8641 ccgaagaccc cgcgttcgcc gccgtcacag agaagtacgc cgcgaagctg accgagctgg
     8701 cgagcgtcgt cgggcgcacg gaggtcgcgc tcgaagcgcg cagcgagccg agccgcaccg
     8761 tcgagacgaa cgccgccgac ttcatcgccg acgccttccg ccgggccgcc tccgccgacg
     8821 tggccctcct caacggcggc tcgatccgcg ccgacaccgt catctcggcg ggcgacctca
     8881 cgctgcgcga cgtgctctcg atcctcccct tcggcaacga cctcgtgaag atcgaggtca
     8941 cgggcgacgt gctccggcag gcgctcgaac acggcgtcag ccgcaccggc cccggctccg
     9001 aaccgggccg cttcccgcag gtctccggcc tgcgctactc tttcgacgcg acgctcacgc
     9061 cgggcgcccg cgtcaagaca gtgacggtcg gcgaccagcc cctcgacgcg aagcgaaagt
     9121 acacgctcgt caccacgcgc tacgtcgcgg agggcggcga ccagtacgcg atgctcaagg
     9181 ggcacccgaa cgtgctgaag gagaagctga tcgacaacga ggtgctgcgc cgcgccatcg
     9241 ccgacgccaa agtgatcgcg ccgcgcacgg acggtcgcct ccagcgcctc gacaagcccg
     9301 ccgacgagaa gccgtgcaag gtggcggcgg cggcaggcgg caagtagtac gcgcgttatg
     9361 aagtccggct tgaaggtcgg acttcgtaac gcgcgagttg aggttttgcg gttttatgtt
     9421 tgacgaattc gacgacgccc cgcccagcgc gaagacgcgc gccatcgagc ttttcgagag
     9481 cgcctaccgc gcgcaggtcg agaaggacta cgacgccgcc atcgaactct accgccgctc
     9541 catcgagacc tacccgaccg ccgaggcgca caccttcctg ggatgggtct acagctttca
     9601 ggagcgctac caggacgcca tcgacgagtg cctcgaagcc atccgcgtgg acgccagcct
     9661 cggcaacccc tacaacgaca tcggcagcta cctcatcgcg cagggcgacc tctggtcgtg
     9721 cgtccgctgg ctcaagctcg cgctgcacgc cccgcgctac gaggcccgcg ccttccccca
     9781 cttcaacctc gcgcgcgtct acgagcagcg ccaccgcccc ctcgaagccg cccgccacta
     9841 cggcctcgcc ctcgaacagc agcccgactt ctcgcaggcc acccgcgcgc tccgccgctt
     9901 gcaggcgcgg ctgaactgat ttcggatatc ggatttcgga ttcacggttg acgattgatg
     9961 acggcaccgc tcccatcgat catccatctt caatccccga ccgctcactt tacttatgag
    10021 gtttcgtgct gctcgatgct cggcaggtct ttgactttgt ctcggtcgat ccgcatcttg
    10081 cggcggttgg cgtccacggc gacgtagacg acctcggcct ccgtgacttt gatgcgctcg
    10141 gaggtgccgc cgtagcgctc ggcctcgacg acgacgcgga cggtgatcga ggtggtgccg
    10201 atgcgctcgg tctcggcgta aaagctgacg aggtcgccga cgtagacggg ttcgtggaag
    10261 acgacctcgc gcatggcgac cgtgacgaag cgctcgatgc ctgtgcggcg gtgcgcctcg
    10321 acggcgccgg cgatgtcgat gtagctgagg atgatgccgc cgaagatggt gccctgcggg
    10381 ttggtgtcgc gcggcaggag cgtgatgcgg atggccgggt tgccgtatga gttcatggtc
    10441 ttttgtctga cggaattgga tgaaaattct agcggcgagc atgagcgccc gccgcgcgcc
    10501 gtgtcaagcc ggcccgccgc cgcaggcggt cgccgccgcg ctacggcggg tcgcaccggg
    10561 cggcgatggc gtacaacggg agccgcctcg gcgcgcatca aaaaactcgc cgtaatagga
    10621 gaaacgcggt cgtttttccc tgacacctgc ccgtcgtctt cccgcacgcc gcgggcaacg
    10681 cccgcagggc ctcgccggga acaagcgtga aaacgcagac acgccgcgcg aggaaatggc
    10741 gctataattc aggctacaat cttccgtgaa ctccgggcgc gggccgcttc gccgccgtcg
    10801 gttaggaacc ttagagatga aaagactatt gactcgctcc gcattgctgc tcgcactact
    10861 gaccctctcg aacgccgcgc ctcgggcggc ggcgcaggac aggcgccccc agcggcgcgg
    10921 cgcgcaggcc gcgcggccca cgccgacccc gacgccgcag ccgaccccgg cggccaccac
    10981 ccagctccgc gaggcgccgc tcccgcccgg cgggcgcgcg cgcttcgacg tgaaccacta
    11041 ccgcatcgag gccgagctgc tgcccggcca gcaccagctc aacgccgtcg gcgacgtgac
    11101 gttcacgccg cgcgacaaca cgcgctcggt catcttcgag ctgaacggct cgctcaaggt
    11161 cgagggcatc gagcggaacg gcaagccgat catcaacttc gtgcaggacg ccgccggcta
    11221 cgacacgacg gggccgaacg tccgcgtcga tctcggcgag gtggtcgccg ccggccagcc
    11281 cgttaccttg cgcttcaagt ggtcaggctc gctcgtcacg cccgagggcg gcccgctcgc
    11341 cgcgaagcgg ctggccttcg tcggcacaga cctctcgtac ctgatgtacg ccgcgcgctg
    11401 gttccccttc cacgactacg ccgccgaccg cgcgacctcg gagatgacca tcaccgtgcc
    11461 gcaagggtac acggtcgcgg gcgcgagcga cgaggccgtg acgccgcggc ccgcgggggc
    11521 gaaggtcgct taccgcttcg tccacaaaca gcccgcgctc gtcggcaact tcgctgccgc
    11581 gcgctacgtc tcgcgcacgc tgcgcatggg cggcttcgag atgacgttcc atgtgctgcc
    11641 cggcagcgag cgcttcgtcg aggcttacgc gcaactgatg ggcgacgcgg tcgagttcta
    11701 cacgcacaag tacggccagc ccgcctcggg cgcccgtctc gtcgtcgcgc agatcgacga
    11761 cgacagcctc gacgcctacg ccgcgcccgg catgctcttc ctctcggcgc gctacttcag
    11821 cggcgcgggc gggacgcagc agcaggcgat ggacgagcgc gtccagcgcg agctggctta
    11881 ccagtggtgg ggccacaccg tgggtctgaa gaccttcgac gacgcgtggc tctcgcaggg
    11941 gctggcggag tggagcgcct tcgcgctgcg cgagcagaag ctgcaaggcg cggcgctcga
    12001 aagcgcgcag cgcgacatgc tcgagcgcgc gctgatgttc gagcagtcgg cctcgatcgc
    12061 gcgcgcgccc tcgacgctcg acgaccagtc ggcggcctac caggcggtcg tcttctacaa
    12121 gggcgcgatg gtcttccgca tgctgcgcga gacgctgggg ccgcagaagt tcgacgagct
    12181 gatgcgccgc ttctacaacg agaaccgcgg caagaacgcc tccatcgacg acttcgagcg
    12241 ggtggcgacg accgtccact ccgacaacct gcgctacttc ttcgcgcgct gggtcgaggg
    12301 cacgggcgtc cccgagttca cctccgatta ccagatcatc cgcacgcgcg gcggcaagtt
    12361 ccgcgcgcgc ggcaccgtca agcagaacac cgagaacctc aagctcccca tcgacgtgat
    12421 gctccgcgcc gagggcgacg acgcctcggc caccgtctac gtcgccgacc gcagcgagga
    12481 cttcgacttc gagtcggcgg gcaagcccct ggagatcatc atcgacccga acaacaaggt
    12541 gctgcgcacg tcggaagagc tgcgcgtctc ggtggtcgct cggcgcggcc ttgagctgtt
    12601 ccgcgagggg cagtacctcg aagcgcagcg gcagttggag gaggcgctca agctcgacaa
    12661 gtcgaactcg tgggtctact acaacctcgg gctcatcttc ttagagcagc gcaactacca
    12721 gcaggcgctc gacaact
//
LOCUS       DBNGOH010000635         3879 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3575972, whole genome shotgun sequence.
ACCESSION   DBNGOH010000635 DBNGOH010000000
VERSION     DBNGOH010000635.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3879)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3879)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3879
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3575972"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1096)
                     /locus_tag="AC159P_12410"
     CDS             complement(<1..1096)
                     /locus_tag="AC159P_12410"
                     /inference="COORDINATES: protein motif:HMM:NF020123.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HIR3696294.1"
                     /translation="MKKRLFLFHSLFIGAALALGVGGAPARALGQQQQRQTVAPKREA
                     SKTAAGKPAPDKPKVTEIKEAGLKSLLEASVAKNRPLLVNFWATWCTPCREEFPDLVA
                     LRGLFAEDALDFVTVSLDDPSDIDTTVPAFLGEMRAHKMPAYLLNAVDQVAAITLVDQ
                     EWLGSDGKQPNKSWQGELPATYLFDRAGKVAFKHTGRIKPAELRAAVEKVLAGPQTPP
                     AKADTSAAAPPAQLLAYDASVPLDVKTVGTEREGGVSVEDITFQGLGETIAAYVVRPT
                     EGAGPFAGVLFVHWFAPPDPTSNRTQYLDEAKALARRGTVSLLVSTFWSDDARYRARR
                     WETDYQNSITQAKNLRRALDVLLSRPGVDAR"
     gene            complement(1297..1896)
                     /locus_tag="AC159P_12415"
     CDS             complement(1297..1896)
                     /locus_tag="AC159P_12415"
                     /EC_number="1.8.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF020123.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HIR3696295.1"
                     /translation="MKKLYTCALLFAFALTANAASLFAGGDTPKAAVGSTVADFKLPD
                     ADGEEHALSALKGKNGTVLIFVSTNCPVSNAYNERMAKLAEEMKARGVAVVGINANVG
                     ETAADIKQHSAEHKLSFPILKDGGNRIADLLGAAVTPEAFYLDAQSKLVYKGRIDNSR
                     NGDNITASELRDAVEATIAGKPVEKTEVRAFGCSIKRAS"
     gene            complement(2230..>3879)
                     /locus_tag="AC159P_12420"
     CDS             complement(2230..>3879)
                     /locus_tag="AC159P_12420"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF012772.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HIR3696296.1"
                     /translation="FSPRWKAMLGHAEAEIDARPEEWFERIHPEDVESFRRHLQEHVE
                     GLTDHFECEHRMRHKTGTWRWMLARGIAVRDARGRATRIAGSQTDTTGRKRVEEQLTH
                     DAFHDALTGLPNRGLFMEHLRLVIEQTKRDRDNLFAVLFLDMDRFKIVNDSLGHIAGD
                     CLLVEISWRLRQCLRGGDIVARLGGDEFTILLRDTEEPGEAALVAARVHAELARPFDI
                     GGNEVYATASIGIVLSSIGFTRPEDFLRAADTAMYRAKAHGRGHHAVFDQEMHARVML
                     TLQLENDLRRAIERGEMIVRYQPIVALDTGRVTGFEALVRWQHPERGLIPPMEFIPVA
                     EEAGLIVPLGTWVLTEACREARRWQGAAAGGPWPDVSVNLSGKQFAQPDLLERVGQVL
                     AETGLDGHALRLEITESVVMENAEATAASLQQLRELGVRLVVDDFGTGYSSLSYLQRF
                     PISTLKIDRSFVSRMCDSEENSEIVRTIIALARNLGIEVVAEGVETPEQLAQLRALGC
                     GYAQGFLFSDAVPADEAADMIARGGDAFTGRVRRPRVLQGR"
BASE COUNT          586 a         1218 c         1381 g          694 t
ORIGIN      
        1 ggcgcgcgtc aacgccgggc cgcgagagca gcacgtcgag cgcgcgccgc aggttcttcg
       61 cctgcgtgat cgagttctgg tagtcggtct cccaacggcg tgcgcggtag cgcgcgtcgt
      121 ccgaccagaa cgtcgagacg agcagagaga ccgtgccgcg gcgcgcgagc gctttggcct
      181 cgtccagata ctgcgtgcgg ttggaggtcg ggtcgggcgg cgcgaaccag tggacgaaga
      241 gcacgcccgc gaacggcccg gcgccctcgg tcggcctcac gacgtaggcc gctatcgtct
      301 cgcccaagcc ctgaaaggtg atgtcctcga cgctcacgcc gccctcccgc tccgtgccga
      361 ccgtcttcac gtcgagcgga acgctcgcgt cgtaggcgag cagttgtgcg ggcggcgcgg
      421 cggcgctcgt gtcggccttc gcgggcggcg tctgcgggcc ggcgagcacc ttctcgacgg
      481 cggctcgcag ctcggcgggt ttgatgcggc ccgtgtgttt gaaggcgacc ttgcccgcgc
      541 ggtcgaagag gtaggtggcc ggaagctcgc cctgccaact cttgttgggc tgcttgccgt
      601 cggagccgag ccattcctga tcgaccaacg tgatggcggc gacctgatcg acggcgttca
      661 gaaggtaggc gggcatcttg tgtgcgcgca tctcgccgag gaaggcgggg acggtcgtgt
      721 ctatgtccga agggtcgtcg agcgagacgg tgacgaagtc gagcgcgtct tcggcgaaga
      781 ggcccctgag cgccacgagg tcggggaact cctcgcggca gggcgtgcac cacgtcgccc
      841 agaagttgac gaggagcggg cggttcttgg ccacgctcgc ttcgagcagc gacttcaacc
      901 ccgcctcctt gatctccgtc accttcggct tgtcgggcgc cggtttgccc gccgcggtct
      961 tcgacgcttc ccttttcggc gcgaccgtct gccgctgctg ctgctgcccc agcgcgcgcg
     1021 cgggggcgcc gcccacgccg agtgcgaggg cggcgccgat gaagagggag tggaagagga
     1081 acagccgctt cttcatgctc gtttaagttc gtccttcggc cttagagcgt ctaacaagac
     1141 tgggcgatta tttgtagggg cagggcaagt ccgcggcccg ctgcaatgca gtcactcggc
     1201 gttgaatcaa tcgagactca agggcaggga caagccctgc ccctacagtt attccggtct
     1261 cgttagtcgc ttccaagact cttaagccct gcgtcgttac gacgcgcgct tgatggagca
     1321 gccgaaggcg cgcacctcgg tcttctcgac gggcttgccg gcgatggtgg cctcgacggc
     1381 gtcgcgcagc tccgaggcgg tgatgttgtc gccgttgcgc gagttgtcga tgcggccctt
     1441 gtagacgagc ttcgactgcg cgtcgaggta gaaggcttcg ggcgtgaccg ccgcgccgag
     1501 caggtcggcg atgcggttgc cgccgtcctt gaggatgggg aacgagagct tgtgctcggc
     1561 ggagtgctgt ttgatatcgg cggcggtctc gccgacgttc gcgttgatgc cgacgacggc
     1621 gacgccgcgc gccttcatct cttcggccag cttcgccatc cgctcgttgt aggcgttcga
     1681 gaccgggcag ttcgtcgaga cgaagatgag gacggtgccg ttcttgccct tcagcgcgga
     1741 gagcgcgtgc tcttcgccgt cggcgtcggg cagtttgaag tcggcgacgg tcgagccgac
     1801 ggcggccttc ggcgtgtcgc cgcccgcgaa gagcgacgcc gcgttggccg tcagcgcgaa
     1861 ggcgaacagg agcgcgcacg tgtagagttt cttcatggct ggttcaggtc aaactccttt
     1921 tcgaaatttc cttccgtgga agaagcaaaa gagtaaccaa tcgaagttca aagttcaaag
     1981 ctcgcggctc gaagttttac atcaacttta catcgacgcc cgtttatcac gatgacgggg
     2041 gaattaacca cgaaggcacg aaggacacga agaagcaggc aagaagaaga gaggaaaaga
     2101 gaaagagccg ccgcccactc tctttgtttt tttccggctt cttctttttt cttccttcgt
     2161 gtctttgtgg ttaatttttt ccggcgtgcg ttcgtggtta attcttactg ccgcgtgttc
     2221 gcgctcgcgt cagcgcccct ggaggacgcg cgggcggcgg actctgccgg tgaaggcgtc
     2281 gccgccgcgg gcgatcatgt cggcggcctc gtccgccggg acggcgtcgg agaagaggaa
     2341 cccctgcgcg tagccgcacc ccaaggcgcg cagttgggcg agctgttcgg gcgtctcgac
     2401 gccctcggcg acgacctcga tgccgaggtt gcgcgcgagc gcgatgatgg tgcggacgat
     2461 ctcggagttc tcctcgctgt cgcacatccg cgagacgaag gagcggtcga tcttcaaggt
     2521 cgagatgggg aagcgctgga ggtaggagag cgaagagtag ccggtgccga agtcgtccac
     2581 gacgagccgc acgcccagct cccgcagctg ttggagcgag gcggcggtcg cctcggcgtt
     2641 ctccatgacg accgactcgg tgatctcaag ccgcagcgcg tggccgtcga gtccggtctc
     2701 ggcgagcacc tgcccgacgc gctccaagag gtccggctgc gcgaactgct tgcccgagag
     2761 gttgacgctc acgtcgggcc aggggccgcc cgccgccgcg ccctgccagc gccgcgcctc
     2821 gcggcacgcc tccgtcagca cccacgtgcc caaagggacg atgaggcccg cctcttcggc
     2881 gacggggatg aactccatcg gcgggatgag gccgcgctcg gggtgctgcc agcgcacgag
     2941 cgcctcgaag ccggtgacgc gccccgtgtc gagcgcgacg atgggctggt agcggacgat
     3001 catctcgccg cgctcgatgg cgcgccggag gtcgttttcg agttgcagcg tgagcatcac
     3061 gcgcgcgtgc atctcctgat cgaagaccgc gtggtggccg cgcccgtgcg ccttcgcccg
     3121 gtacatggcg gtgtcggcgg cgcgcaggaa gtcttcgggg cgcgtgaagc cgatggacga
     3181 gagcacgatg ccgatgctgg cggtggcgta gacctcgttg ccgccgatgt cgaaggggcg
     3241 ggccagctcg gcgtggacgc gcgcggcgac gagcgcggcc tcgcccggct cctcggtgtc
     3301 gcgcagaagg atcgtgaact cgtcgccgcc gagccgcgcc acgatgtcgc cgccgcgcag
     3361 gcactggcgc aggcgccaac tgatctcgac gagcaggcag tcgcccgcga tgtggcccag
     3421 agagtcgttg acgatcttga agcgatccat gtcgaggaag agcacggcga agaggttgtc
     3481 gcggtcgcgc ttcgtctgct cgatgacgag ccgcaggtgc tccatgaaga ggccgcggtt
     3541 gggcagcccg gtgagcgcgt cgtggaaggc gtcgtgcgtg agctgctctt cgacgcgctt
     3601 gcggcccgtg gtgtcggtct gcgagccggc gatgcgcgtg gcgcggcccc gcgcgtcgcg
     3661 caccgcgatg ccgcgcgcga gcatccagcg ccaggtgccg gtcttgtggc gcatgcggtg
     3721 ctcgcactcg aagtggtctg tgagaccttc gacgtgctcc tgaagatggc ggcggaagct
     3781 ttccacgtct tcggggtgga tgcgctcgaa ccactcctcg gggcgcgcgt cgatctcggc
     3841 ttcggcgtgg ccgagcatcg ccttccagcg cggcgagaa
//
LOCUS       DBNGOH010000636         5717 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4859417, whole genome shotgun sequence.
ACCESSION   DBNGOH010000636 DBNGOH010000000
VERSION     DBNGOH010000636.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5717)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5717)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5717
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4859417"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            328..441
                     /gene="rpmJ"
                     /locus_tag="AC159P_12425"
     CDS             328..441
                     /gene="rpmJ"
                     /locus_tag="AC159P_12425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HIR3696297.1"
                     /translation="MKVRASVKKMCDNCKVIHRKGVVRVICTNPKHKQRQG"
     gene            576..959
                     /gene="rpsM"
                     /locus_tag="AC159P_12430"
     CDS             576..959
                     /gene="rpsM"
                     /locus_tag="AC159P_12430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006902862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HIR3696298.1"
                     /translation="MARVVGVDLPVNKRIEVGLTYIYGIGRSRSTAILREADVATDIR
                     VRDLDEAQLNRIRQVIEGQGEVEGDLRKRVQMDIKRLMDIGCYRGLRHRRGLPVRGQR
                     THTNARTRKGPRRATIAKKKAPGKK"
     gene            1089..1493
                     /gene="rpsK"
                     /locus_tag="AC159P_12435"
     CDS             1089..1493
                     /gene="rpsK"
                     /locus_tag="AC159P_12435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HIR3696299.1"
                     /translation="MAKATTGGKKKVFRKKEKKNIPVGIVHIAASFNNTLISITDTEG
                     NLIAQSSSGARGFRGSRKGTPFAAQQAAYEAANKAKDAGMTQCEVCVKGPGGGRESAI
                     RAINSAGIRVMLIRDTTPIPHNGCRPPKRRRV"
     gene            1677..2318
                     /gene="rpsD"
                     /locus_tag="AC159P_12440"
     CDS             1677..2318
                     /gene="rpsD"
                     /locus_tag="AC159P_12440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HIR3696300.1"
                     /translation="MARYRGAVCRLCRREGAKLFLKGDRCFKPSCPIEKRGTQPPGQH
                     GNSLRRGKALVGYGQQLREKQKVKRIYFVLEGQFRNYFEKAARIKGITGENLLVLLER
                     RLDNAVYRAGFATSRRQARQLVNHGHIEVNGRKVDIPSFQVKQGDTISVKEASRKNPH
                     VEGAWQTAGGRGRPSWVAPAEGAEMSARVASLPTREDIDRSINEQLIVELYSK"
     gene            2414..3469
                     /locus_tag="AC159P_12445"
     CDS             2414..3469
                     /locus_tag="AC159P_12445"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014264255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HIR3696301.1"
                     /translation="MAIDQHNLWTGFQMPRRLAVEGETLTERYGRFSAQPFERGFGTT
                     IGNSLRRALLSSIEGAAITAVKIEGVEHEFSSIRGVVEDATDVILNLKQIPFKLHGNE
                     PKTLTITKDGAGEVTSGDIETDADVEILDQNVYIATVSEGGSLRVEMRLKRGRGYVPA
                     ERNFDEDLSLGYIPIDSVHTPVKKVNYAVEAARLGQNTEFDKLTIEVWTDGSVKPDDA
                     IGLAAKLIKDHMAIFINFEEDTDEFAYQNIERPPLPRNDVLDRSVDELELSVRSYNCL
                     KNANIRSIRDLVQRSEREMLATKNFGKKSLNEIKDILHGMGLDFGMAFDEQGNPIPGS
                     GGSDIDLSEYADDEDDE"
     gene            3586..4284
                     /gene="rplQ"
                     /locus_tag="AC159P_12450"
     CDS             3586..4284
                     /gene="rplQ"
                     /locus_tag="AC159P_12450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012096720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HIR3696302.1"
                     /translation="MRHLKAHRKLGRTSEHRNSMLRNMATSLINARDERIVTTLPKAK
                     ELRSYVERVITLSRKAANLEGDDVVAQALHLRRQAAAFFHAGNRQQAPSAGKRKQPRA
                     PRTAGVAALKRLFDELGERYKDRPGGYTRILKLGRRDGDNAEIAIIELVDNPRERESA
                     EAATKRAKSATSGKKKTAKASESKAAEGGKSGGRKAAGKGKASAEEAEQDKAGSGGRR
                     SKKTESTAAEQETE"
     gene            4479..5198
                     /gene="accB"
                     /locus_tag="AC159P_12455"
     CDS             4479..5198
                     /gene="accB"
                     /locus_tag="AC159P_12455"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HIR3696303.1"
                     /translation="MSEHDQVTATGREHSNETADANSERSGGGERGGPSGRHGGGRRG
                     RRGGGHTPQQPQRQQQGQQRRADSSLNMDELRELTELFTAHGLTDFEFENAEIRVRLS
                     RNPAPQAGGGGAGAAFQNPASALTHAATAPATGGGSAAQAAPPPPTTTEAPRVEEKAE
                     DLHIITSPIVGTFYRSPSPDAEPFIRVGSAVEPDTVVCIIEAMKLMNEIQSETAGTVE
                     KIYVENGQPVEYGQPLFGVKK"
     gene            5326..>5717
                     /locus_tag="AC159P_12460"
     CDS             5326..>5717
                     /locus_tag="AC159P_12460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015351880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HIR3696304.1"
                     /translation="MRKFRKILIANRGEIACRIIWTCKEMGISTVAVHSTADRDSLHV
                     RFADEAVCIGQATSAQSYLNIPSVIAAAEITNVDAIHPGYGFLAESAYFAEICEACNI
                     KFIGPRPSVIRLMGDKVEARRTMKEAGLP"
BASE COUNT         1200 a         1813 c         1863 g          841 t
ORIGIN      
        1 ggcacgcaca agaccaagac gctcaaggac ggctggacgg tcgtcaccct ggacggcagg
       61 ccgagcgccc acgtcgagca caccatcgcc atcaccgaag aggggccgga ggtgctgccg
      121 caggctgccc ggaaagatgt cccggaggcc gaggcggcgg tcgccccctg aggaagtcgg
      181 cgccgcggct tggagcgcgg cggtaagtgt gttactattc gcaatttgcc agacacgccg
      241 ggttttcagc cgggctgtac agtgcggtgt ggcgagagaa gaagcagcag agagcgggtc
      301 gcggcgaaag acattgaggt gtgaacgatg aaggtacggg cttcagtcaa gaagatgtgc
      361 gacaactgca aggtcatcca ccgcaaaggc gtggtgcgcg tgatctgcac gaaccccaag
      421 cacaagcagc ggcaggggta gcgagcagtt ttcagtttcg agttttcagt gaaggcaaaa
      481 cgccgggcgc accggcgggc cgctgctgaa aaccgacagg ctgaaaactg aaactcaaaa
      541 ctgaaaactg tttttcgaga ttcggagtta agagcatggc tcgtgtagtt ggcgttgacc
      601 tcccggtcaa caagcggatt gaggtcggcc tgacgtacat ctacggcatc ggtcgctcgc
      661 gttcgaccgc gatcttgcgc gaggcggatg tcgccacgga catccgcgtc cgcgatctgg
      721 acgaggcgca gttgaaccgc atccgccagg tcatcgaagg gcagggcgag gtcgagggcg
      781 acctgcgcaa gcgcgtccag atggacatca agcggctgat ggacatcggc tgctaccgcg
      841 gcctcagaca ccgccgcggc ctccccgtgc gcgggcagcg gacgcacacc aacgcgcgca
      901 cccgcaaggg gccgcgccgc gccaccatcg ccaagaagaa ggcgccgggc aagaagtagg
      961 gaagccgtta gctattagct attagctatt agctttgacg tggtggcggg cggctgagga
     1021 ttaaatgaag gcggttggct tcgccattta tggctaacag ctaacagcta atggctaaga
     1081 gctaatttat ggcaaaagca acaacgggcg ggaagaagaa agtcttcagg aagaaagaga
     1141 agaagaacat ccccgtgggg atcgtgcaca tcgcggcgtc gttcaacaac acgctgatct
     1201 cgatcaccga caccgagggg aatttgatcg cgcagtcgtc gtcgggcgcg cgcggcttcc
     1261 gcggctcgcg caagggcacg ccgttcgccg cgcagcaggc cgcctacgag gccgcgaaca
     1321 aggcgaagga cgccgggatg acgcagtgcg aggtgtgcgt caaggggccg ggcggcgggc
     1381 gcgagtccgc gatccgcgcc atcaacagcg ccggcatccg cgtcatgctc atccgcgaca
     1441 cgacgcccat cccccacaac ggctgccgcc cgccgaagcg gcgccgggtg taagaatttc
     1501 ggattgcgga ttgcggattt cggattagaa agagcaggta cgcttttcta atccgaaatc
     1561 cgtaatccgc aatccacaat cgtgcgacct aaaggacgaa gggccgcgcg ccgcggtggt
     1621 ttggcgcgtg gtccgtaaac ttttccgtct gtaacttaga ctggaggcaa aaagaagtgg
     1681 ctaggtatcg tggtgcggtg tgccgtctgt gccgccgcga aggcgcgaaa ctgtttttga
     1741 agggcgaccg ctgtttcaag ccgtcgtgcc cgatcgagaa gcgcggcacg cagccgcccg
     1801 gacagcacgg caactctttg cgccgcggca aggcgctggt cggctacggc cagcagctcc
     1861 gcgagaagca gaaggtcaag cgcatctact tcgtcctcga aggccagttc cgtaactact
     1921 tcgagaaggc ggcccgcatc aagggcatca cgggcgagaa cctgctggtg ctgctcgaac
     1981 ggcgtctcga caacgccgtc taccgcgcgg gcttcgccac gtcgcgccgt caggcccgcc
     2041 agctcgtcaa ccacgggcac atcgaggtca acgggcgcaa ggtggacatc ccctcgttcc
     2101 aggtcaagca gggcgacacg atcagcgtca aggaggcgag ccgcaagaac ccgcacgtcg
     2161 agggcgcgtg gcagaccgcc ggcgggcgcg ggcgccccag ttgggtcgcc cccgccgagg
     2221 gcgccgagat gagcgcgcgc gtggcctcgc tcccgacgcg cgaggacatc gaccgctcga
     2281 tcaacgagca gttgatcgtc gagctttata gtaagtaacc cggacgcggc gcgcgcgcac
     2341 agtcactctc gattgtgcgc gcggcgccgc tcgcattgta agtggctttt cagacgggga
     2401 tacgaaaaac atcatggcaa tcgatcagca caatctctgg acaggattcc aaatgccgcg
     2461 ccgcctcgcc gtcgagggcg agacgttgac cgagcgctac gggcgcttct cggcgcagcc
     2521 gttcgagcgc ggcttcggca ccacaatcgg caactccctg cggcgcgcgc tgctctcctc
     2581 gattgagggc gcggccatca ccgccgtcaa gatcgagggc gtcgagcacg agttctcttc
     2641 gatccgcggc gtggtcgagg acgcgaccga cgtgatcctc aacctcaagc agattccgtt
     2701 caagctccac ggcaacgagc cgaagacgct gaccatcacg aaggacggcg cgggcgaagt
     2761 cacctccggc gacatcgaga ccgatgcgga cgtggagatt ctcgaccaga acgtctacat
     2821 cgccaccgtc agcgagggcg gcagccttcg cgtcgagatg cgcctcaagc gcgggcgcgg
     2881 ctacgttccc gccgagcgga atttcgacga ggacttgagc ctcggctaca tccccatcga
     2941 ctccgtccac acgccggtca agaaggtcaa ctacgcggtc gaggccgcgc gcctcggcca
     3001 gaacacagag ttcgacaagc tgaccatcga agtttggacg gatggctcgg tgaagccgga
     3061 cgacgccatc ggcctcgccg cgaagttgat caaggatcac atggcgatct tcatcaactt
     3121 cgaggaggac acggacgagt tcgcctacca gaacatcgag cgcccgccgc tgccgcgcaa
     3181 cgacgtgctc gaccgctcgg tggacgagct tgagctgtcg gtgcgctcgt acaactgcct
     3241 gaagaacgcc aacatccgct cgatccgcga cctcgtgcag cgctcggagc gcgagatgct
     3301 ggcgacgaag aacttcggca agaagtcttt gaacgagatc aaggacatcc tccacgggat
     3361 gggcctcgac ttcggcatgg ccttcgacga gcagggcaac ccgatccccg gctcgggcgg
     3421 ctcggacatc gacctcagcg aatacgcgga cgacgaagac gacgagtaag cgaagtgatg
     3481 aatgatgaat gcggaacgat gaatgagaag cacggtgcct ctcatcaaac aagccgcttc
     3541 tcattcatca ttcatcactc cgcattcatc atttaggata ttgtcatgcg acacttaaag
     3601 gcacaccgga aactcgggcg gacgagcgag caccgcaact cgatgctgcg gaacatggcg
     3661 acctcgctca tcaacgcgcg cgacgagcgc atcgtgacga cgctgccgaa ggccaaagag
     3721 ctgcgctcgt atgtcgagcg cgtcatcacc ctctcgcgca aggccgcgaa cctcgaaggc
     3781 gacgacgtgg tcgcgcaggc tttgcacctc aggcgtcagg cggcggcgtt cttccacgcg
     3841 ggcaaccgcc agcaggcgcc ttcggcgggc aagcgcaagc agccgcgcgc gccgcgcacg
     3901 gcgggcgtcg ccgcgctgaa gcgtctgttc gacgagctgg gcgagcgtta caaggatcgt
     3961 ccgggcggct acacgcgcat cctgaagctc gggcggcgcg acggcgacaa cgccgagatc
     4021 gccatcatcg agctggtcga taacccgcgc gagcgcgagt ccgcggaggc cgcgaccaag
     4081 cgcgccaagt ccgccacttc gggcaagaag aagacggcga aggcttcgga gtcgaaggcc
     4141 gcggagggcg ggaagagcgg cggcaggaag gccgccggca agggcaaagc gtccgccgag
     4201 gaggccgaac aggacaaggc gggcagcggc ggtcgccgct cgaagaaaac cgaatcaacc
     4261 gccgccgagc aggagacgga gtaacatccg ggtgacacgg gggatgggcg ctcccctgct
     4321 cgcactttga gtcccgagcg cccggcaccg cccgtacacc ctcaagcaag aaagccccaa
     4381 gcgcaccgct cgctcggcaa gcggcctggg gcttttctct tgtcggcgcg cgggcacgca
     4441 cgcacccggc gcgaacttcg gacggagacc acacgagcat gagcgaacac gatcaagtca
     4501 cggcgacggg acgcgagcac tcgaacgaaa ctgcggacgc taactcggag cggtcgggcg
     4561 gcggcgagcg cggcggcccg tcgggtcgcc acggcggcgg tcgtcgcggc aggcgcggcg
     4621 gcggtcacac gccccagcag ccgcagcgtc agcagcaggg ccagcagcgc cgcgcagact
     4681 cctcgctcaa catggacgag ctgcgcgagt tgaccgagct gttcacggcg cacggcctga
     4741 ccgacttcga gttcgagaac gcggagatac gcgtccgcct gagccgcaac cccgcgccgc
     4801 aggcaggcgg tggaggcgca ggggccgcgt tccagaatcc cgcgtcggcg ttgacgcacg
     4861 cggccaccgc gcccgcgacc ggcggcggct ccgccgccca agctgcgccg cctcccccga
     4921 cgacgaccga ggcgccccgc gtggaggaga aggccgaaga cctgcatatc atcacctcgc
     4981 ccatcgtcgg caccttctac cgctcgccct cgcccgacgc cgaacccttc atccgcgtcg
     5041 gcagcgccgt cgagccggac accgtcgtct gcatcatcga ggcgatgaaa ctgatgaacg
     5101 agatacagtc cgagaccgcc ggcaccgtcg aaaaaatcta cgtcgagaac ggccagccgg
     5161 tcgagtacgg ccagccgctc ttcggagtca aaaaataaag tgatgaggac tgatgaaaag
     5221 ccgtgctagg taaaccgtga accgtgacaa gaaaaaaccg cttctgactc gtcacggttt
     5281 agggttcacg acttacggcc tttactcagt cctcagtcct tgagtatgcg aaagttcaga
     5341 aaaatcctga tcgcgaaccg gggcgagatc gcgtgccgca tcatctggac gtgtaaggag
     5401 atgggcatct cgacggtcgc cgtgcattcg accgccgacc gcgactcgct gcacgtgcgc
     5461 ttcgccgacg aggccgtgtg catcgggcag gccacctcgg cgcagagcta tctgaacatc
     5521 ccttcggtta tcgccgccgc cgagatcacg aacgtcgatg cgatccatcc cggctacggc
     5581 ttcctcgccg agtcggccta cttcgccgaa atctgcgagg cctgcaacat caagttcatc
     5641 ggcccgcgcc cttcggtgat ccgcctgatg ggcgacaagg tcgaggcgcg gcggacgatg
     5701 aaagaggcgg ggctgcc
//
LOCUS       DBNGOH010000637         6953 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4125938, whole genome shotgun sequence.
ACCESSION   DBNGOH010000637 DBNGOH010000000
VERSION     DBNGOH010000637.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6953)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6953)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6953
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4125938"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..251)
                     /locus_tag="AC159P_12465"
     CDS             complement(<1..251)
                     /locus_tag="AC159P_12465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013164790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HIR3696305.1"
                     /translation="MRKRIAAAGLLMLLVAAAVRAEKKEKAPLRVEPTVDLSRYAGLW
                     YEVARLPNSFQEKCAGDVTAQYAPLGAGRLKVVNRCRKP"
     gene            complement(264..719)
                     /locus_tag="AC159P_12470"
     CDS             complement(264..719)
                     /locus_tag="AC159P_12470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002638149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR3696306.1"
                     /translation="MKRTVSGIERTNEPGRVSIMSEQLAEKFMKALGRLEAAGELEPI
                     VSLFAEECEVGNVVSPEKFRGREGAREFWGAKYRDTFGEVRSTFRNVFAAANRAALEW
                     TTEGTASDGRPVRYDGVSILETEGERITRFRAYFDAGALGRQLTGKAHG"
     gene            complement(716..1615)
                     /locus_tag="AC159P_12475"
     CDS             complement(716..1615)
                     /locus_tag="AC159P_12475"
                     /inference="COORDINATES: protein motif:HMM:NF014646.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing protein"
                     /protein_id="HIR3696307.1"
                     /translation="MRDAVGRKTLTSKEAARLLGVSEASVKRWADGGLLAAERTVGGH
                     RRFRPEAVAAFRRAGAGAARGHAAREEAVGAKRAARIKAAAGKALTDALFESLVEGQA
                     EEAAALFVNLHLRGHTVASIADAVLCPAMRRVGELWRAGEVTVAQEHVATRAALGALR
                     ALRDASGGREPNGLKALCCSVEEDYHELPVRVAALVLEAGGWNALTLGASTPFYALAE
                     AVSRFGPRLVCVASTVFGKLDRAAREYGELRAAARGVGASVVLGGAGFAGAEMRRRFP
                     AELHADSFTQLETFAPTLAGGGG"
     gene            1877..2458
                     /gene="idi"
                     /locus_tag="AC159P_12480"
     CDS             1877..2458
                     /gene="idi"
                     /locus_tag="AC159P_12480"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013812679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HIR3696308.1"
                     /translation="MRAQEEVILVDEQDRVVGAGEKLRAHREGALHRAFSVFVFDRGG
                     RLLLQRRARTKYHSGGLWSNTACGHPRPGEETGAAARRRLREEMNFDCELRASFRFIY
                     RAELDGALVEHEYDHVFVGEYSGQPAPDPSEVEAWLWVQVDDLRRDLRERPWRYSYWL
                     KVVVEGDDGRRLDTLLKEAGGQPISDFKFRISD"
     gene            2706..4244
                     /locus_tag="AC159P_12485"
     CDS             2706..4244
                     /locus_tag="AC159P_12485"
                     /inference="COORDINATES: protein motif:HMM:NF012242.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HIR3696309.1"
                     /translation="MLLGIDYGTTRTVVVAVDRGNYPVVSFQGEHGDPQEWYPSLIAY
                     ARGGAERAYGFEARARQHEEGWSLLRSFKRELGRLGPQAPVPLGEETAPALQLLAEFL
                     TQLRADLYERSNLQLGSEESLEVLVSVPANANSNQRYVTLEAFRLAGFQVRGTLNEPS
                     AAGVEYAHRHLKAGRGASAREHVIVYDLGGGTFDASVISMTDQRHEVLASDGIARLGG
                     DDFDEVLLDMALAEAGIGQVPAEARTALLEECREKKEGLHPNTRKVVVDLGRAIEGAG
                     EVLVGTADFYERCRPLVEQTIEAMERAVADSAEGAHLDWSSVAAVYLVGGSSDLPVVA
                     RMLRERYGRLVRRSPYPHAATAIGAAIAADSEAGYELRERFTRHFGVWREAEGGRAIA
                     LDVIFEKETPLPSNGAQKLTRVRRYRPAHNLGHYRFLECGRVDAGGHPTGDITPWDEI
                     FFPFAAELQHETQLARVPVTRTPPADQLVEEVYSCDEHGIIEVEVINHATQHQRRFML
                     HKTV"
     gene            4578..5153
                     /locus_tag="AC159P_12490"
     CDS             4578..5153
                     /locus_tag="AC159P_12490"
                     /inference="COORDINATES: protein motif:HMM:NF022273.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2760 domain-containing protein"
                     /protein_id="HIR3696310.1"
                     /translation="MIGLGKRISYATRCFFSVLSRGEVPEDIAPEVVKASASTAPALE
                     APPLAVEPSLAPEPAPPVEESTDRAVQMLALLQREGRLIDFFSEDLTPYPDAQVGAAV
                     RELHAGCRKALEQYVTLEPIIEGEEDGPVTVEAGFDPASVKLVGNVTGQPPLRGLLRH
                     RGWRATEVELPPLAPQESARAIVAPAEVEIS"
     gene            5323..>6953
                     /locus_tag="AC159P_12495"
     CDS             5323..>6953
                     /locus_tag="AC159P_12495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HIR3696311.1"
                     /translation="MDTRYIVGIDLGTTNSALAQFDTAAPEEEARIAVEGVPQLVNPN
                     EVEERTLLPSFLYVPGEMDFPKGALALPWEREPQVFAGELARKRGAENPVRLVASAKS
                     WLSYAAVNRAAPILPWGAPEEVRKLSPVEVSAQYLAHLRQAWDARVNAKKNPELALSE
                     QEVLLTVPASFDEEARELTLRAAEQAGLKRVTLLEEPQAAFYAWIDAQGESWRKRIKV
                     GDLILVCDVGGGTTDFSLITVSEENGELSLKRVAVGEHILLGGDNMDLALARLLQQRL
                     EEEGHRVDTWQLQGLWHQCRLAKEKLFAEPKSQKQPVTLLGKGSKLVGGTIKTELRRE
                     DLERVLTDGFFPVVGREEMPAKQRRIGFQELGLPYAADAAVTKHLARFLSQQTAANDA
                     EGDRIRRGPSGFACPTHVLFNGGVMKAAMLRERVVQVLDEWVRAEGFDSLGERGVLEA
                     PDLDHAVARGAAYYGKAKRGRGVRIRSGAPRTYYIGIESAMPAVPGMPAPLKALCVVP
                     FGMEEGTEGQIPGREFGLVVGEPAEFRFLSSTTRR"
BASE COUNT         1151 a         2324 c         2352 g         1126 t
ORIGIN      
        1 ttcggcttgc gacaacggtt gacgaccttc aggcggcccg cgccgagcgg ggcgtactgc
       61 gccgtcacgt cgcccgcgca cttttcctga aagctattcg ggaggcgcgc gacctcgtac
      121 cacaggcccg cgtagcgcga gagatcgacg gtcggctcca cacgcagggg cgctttctct
      181 ttcttctcgg ccctcaccgc cgccgccacc aacagcatta acagaccggc tgccgcgatt
      241 cttttacgca taatcccccc gttctaacca tgtgcctttc ccgtcaactg tcgtccgagt
      301 gcgcccgcgt cgaagtaggc gcggaagcgc gtgatgcgct ccccctccgt ttcgagaatg
      361 ctgacgccgt cgtaccgcac cggcctcccg tcgctcgccg tcccttccgt cgtccattcg
      421 agcgcggcac ggttcgccgc ggcgaagacg ttgcgaaagg tcgagcgcac ctcgccgaag
      481 gtgtcgcggt acttcgcgcc ccagaactcg cgcgcgccct cacgcccgcg aaacttctcc
      541 ggcgagacga cgttcccgac ctcgcactct tccgcgaaga gcgacacgat tggttcaagc
      601 tcgcccgcgg cttcgagcct gccgagtgcc ttcataaact tttccgccaa ctgctccgac
      661 atgatcgata ccctccccgg ttcgttcgtc cgttcgattc ccgacaccgt gcgcttcatc
      721 cgcctccgcc cgcgagcgtc ggggcgaatg tttcgagttg cgtgaagctg tccgcgtgaa
      781 gctccgcggg gaaccgccga cgcatctccg cgcccgcgaa gcccgcgccg cccagcacga
      841 ccgaagcgcc cacgccccgc gccgccgcac gcagctcccc gtactcgcgc gccgcgcggt
      901 cgagcttgcc gaagacggtc gaggcgacgc agaccagccg cggcccgaac cgcgacaccg
      961 cctccgccag cgcgtagaac ggcgtgctcg cgccgagcgt cagcgcgttc cagcccccgg
     1021 cttcgagcac gagcgcggcg acgcgcaccg gcagctcgtg gtagtcctcc tcgacgctgc
     1081 aacagagcgc cttcaggccg ttcggctctc tcccgcccga cgcgtcgcgc agcgcgcgca
     1141 gcgcgccgag ggccgcccgc gtcgccacgt gttcctgcgc caccgtcacc tcgcccgcgc
     1201 gccacagctc gccgacgcgc cgcatcgcgg ggcacagcac cgcgtccgcg atggacgcga
     1261 ccgtgtggcc gcgcaggtgc aggttgacga agagcgccgc ggcctcttcc gcctgaccct
     1321 cgacgagcga ttcgaagaga gcgtccgtca gcgccttgcc ggcagccgcc ttgatgcgcg
     1381 ccgcgcgctt cgcgccgacc gcctcttcgc gcgcggcgtg gcctcgcgcg gcgcccgcgc
     1441 ccgcgcggcg gaaggccgcg acagcctcgg gccgaaacct gcggtggccg cccaccgtcc
     1501 gctccgccgc cagcagcccg ccgtccgccc agcgcttcac cgaagcctcg ctcacgccga
     1561 gcagccgcgc cgcctcttta ctcgtcagag tctttcgacc gaccgcgtct ctcataaagg
     1621 tacgcaaccg actctatcaa aggattcgat tcctgtaaat aaaaatcgtg caaatcgccc
     1681 atcagaatta gattatgacc aattcaatct cgaaactcaa cttgacagca caaaataatt
     1741 cgtctaaatc gttcaatacg atttagacga gaacggccat gcgtttcacg tctgagcgtt
     1801 tcgggggccg ccggttagaa tgctcgtggc cgccgacgaa ccaggcaagg ggtggccatc
     1861 acgcggggag gaagagatgc gggcgcagga agaagtgata ctggtggacg agcaagatcg
     1921 cgtggtcggc gcgggcgaga agctgcgcgc gcaccgcgag ggcgcgctgc accgcgcgtt
     1981 ctcggtcttc gtcttcgacc gcggggggcg gctcctgctt cagcgccgcg cgcggacgaa
     2041 gtaccactcg ggcgggctgt ggtcgaacac ggcgtgcggc cacccgcgac cgggcgagga
     2101 gacaggggcg gcggcgcggc ggcggctgcg cgaggagatg aacttcgact gcgagctgcg
     2161 cgcgtccttc cgcttcatct accgcgcgga actcgacggc gcgctcgtcg agcacgagta
     2221 cgaccacgtc ttcgtcggcg agtattccgg ccagcccgcg cccgacccgt ccgaggtgga
     2281 ggcgtggctc tgggtgcagg tggacgacct gcgccgcgac ctgcgcgaga ggccgtggcg
     2341 ctacagctac tggctgaagg tcgtcgtcga gggcgacgac gggcggcgcc tcgacacgct
     2401 gctcaaggaa gcgggcggcc aaccgatttc ggattttaag tttcggattt cggattagcg
     2461 tgctgcccaa gctcgaagct tgatatcagg cattcatctc tcgatgcccg agcaatccga
     2521 aatccgaaat ccgaaatctt ccgaagcgtc cgcttgacac ggagggccgc ttcgcggaaa
     2581 aagtaacctg acgcgaacgg gccggggccg aatagtccaa gcgcccggac gtggtgtcgg
     2641 gtcgcgggcg gggcttgctc gcccgcgcgg cgttttgaaa ttcacgacgt atcaaggatg
     2701 acgcaatgtt acttgggatc gattacggga cgacgcggac ggtcgtcgtg gcggtggatc
     2761 ggggcaacta cccggtggtc agttttcagg gcgagcacgg cgacccgcag gagtggtatc
     2821 cgtcgctcat cgcgtacgcg cggggcggcg ccgagcgcgc ttacggcttc gaggcgcgcg
     2881 cgcgccagca cgaagagggc tggtctctgc tgcgctcgtt caagcgcgag ctgggcaggc
     2941 tcgggccgca ggcgcccgtc ccgttaggcg aagagaccgc gcccgcgctt cagttgctcg
     3001 cggagtttct cacgcagctc cgcgcggatt tgtacgagcg ctcgaacctg caactcggtt
     3061 cggaggaatc gctggaggtg ttggtctcgg tgcccgcgaa cgcgaacagc aaccagcgtt
     3121 acgtgacgct ggaggcgttc cggctcgcgg gctttcaggt gcgcggcaca ctgaacgagc
     3181 cgtcggcggc gggcgtcgag tacgcgcacc gccacctcaa ggcggggcgc ggcgcgtctg
     3241 cgcgcgagca cgtcatcgtg tacgacctcg gcggcggcac gttcgacgcc tcggtcatca
     3301 gcatgacgga tcagcggcac gaggtgctgg cgagcgacgg catcgcgcgg ctcggcggcg
     3361 acgacttcga cgaagtgctg ctcgacatgg cgctcgccga agccggcatc ggccaagtgc
     3421 cggcggaggc gcgcacggct ctcctggaag agtgccgcga gaagaaggag ggcttgcacc
     3481 cgaacacgcg caaggtggtg gtcgatctcg gtcgcgcgat agagggcgcg ggcgaggtgc
     3541 tggtcggcac cgcggacttt tacgagcgct gccgcccgct cgtcgagcag accatcgagg
     3601 cgatggagcg ggccgtcgcg gactctgcgg agggcgcgca cttggattgg agttcggtcg
     3661 cggcggtgta tctcgtcggc gggtcgagcg atctgccggt ggtcgcgcgg atgctgcgcg
     3721 agcgctacgg gcggctcgtg cgccgctcgc cgtacccgca cgcggcgacc gccatcggcg
     3781 ccgccatcgc cgccgactcc gaggcgggct acgagctgcg cgagcgcttc acgcgccact
     3841 tcggcgtctg gcgcgaggcc gagggcgggc gcgccatcgc gctcgacgtg atcttcgaga
     3901 aggagacgcc gctcccctcg aacggcgcgc agaagctcac gcgcgtgcgc cgctacaggc
     3961 ccgcgcacaa cctcggccac taccgcttcc tcgaatgcgg gcgcgtggac gcgggcggcc
     4021 acccgaccgg cgacatcacg ccgtgggacg aaatcttctt ccccttcgcg gcggagctac
     4081 agcacgagac gcagctcgcg cgcgtccccg tcacgcgcac gccgcccgcc gaccagctcg
     4141 tcgaagaggt ctactcctgc gacgagcacg gcatcatcga ggtcgaggtc atcaaccacg
     4201 ccacccaaca tcaaagacgc ttcatgcttc acaagacggt ctgacggcgg aagggggaaa
     4261 aggggaaaag gggaagaggg gaagagtgag gatgagagga gtgctgcttt cagccctcag
     4321 tgaaacatga gaggcacgct gcttctcatc atccttttcg ccccttcccc ttttcgcccc
     4381 ttcccccttt cttcctacat ttttgtccgc cccgcggtca acatgactgg caccttcttc
     4441 gctttggccg cgtacatctc gacactacca acaacaatct cacgtcgttt gctaaatccg
     4501 tcggaggtct atagcatatt cgtttatttt cccgtcgcga tttcaggcga gagagagagt
     4561 aggaggcatc agccatcatg attggactcg gtaaacgcat ttcttacgcc acgcgctgtt
     4621 tcttctccgt gctttcgcgc ggcgaggtgc cggaggacat cgcgcccgaa gtggtcaagg
     4681 cttcagcgtc aacggcgccc gcgttagaag cgccgccgct ggccgtggag ccttcgctgg
     4741 cgcctgagcc tgcgccgccc gtcgaggaat cgacagaccg cgccgtgcag atgctggcgc
     4801 tgctgcaacg cgaggggcgt ctgatcgact tcttcagcga agacctgacg ccgtacccgg
     4861 acgcgcaggt cggcgcggcg gtgcgcgagc tgcacgcggg ctgccgcaag gcgctcgaac
     4921 agtacgtcac gctcgaaccc atcatcgagg gcgaggagga cggccccgtc accgtcgagg
     4981 cgggcttcga cccggcctcg gtcaagctcg tcggcaacgt gacggggcag ccccccttgc
     5041 gcggcctgct ccgccaccgc ggctggcgcg cgacggaagt cgagctgccg ccgctcgccc
     5101 cgcaagagtc ggcccgcgcc atcgtcgcgc ccgccgaagt ggaaatatcc taagagccgt
     5161 gaaccgtaag ccgtaaaccg tgacaagtga aacgcaagtt acgggtgtcg ggtgatgagt
     5221 gatgagaggg acgctgttcg gactcatcac ggttgacggc ccttcactca tcacggttca
     5281 cggtttacgg ttcacggctg ttgactatcg aggtgaatga tcttggacac acgttacatc
     5341 gtcggtattg atttggggac gaccaattcg gcactcgcgc agttcgacac ggcggcgccg
     5401 gaagaggagg cgcgcatcgc ggtcgaaggc gtgccgcagc tcgtgaatcc taacgaggtc
     5461 gaggagcgga cgctgctgcc ttcgttcctg tatgttccgg gcgagatgga tttcccgaag
     5521 ggcgcgctgg cgctgccgtg ggagcgcgag ccgcaggtgt tcgcgggcga gctggcgcgc
     5581 aagcgcgggg cggagaaccc cgtgcggctg gtcgcgtcgg cgaagtcgtg gctctcttac
     5641 gcggcggtga atcgcgcggc gccgatcctg ccgtggggcg cgcccgaaga ggtgcgcaag
     5701 ctctcgccgg tcgaggtctc ggcgcagtac ctcgcgcacc tgcgtcaggc gtgggacgcg
     5761 cgcgtgaacg cgaagaagaa tcccgagctg gctttgagcg agcaggaagt tctgttgacc
     5821 gtgcccgcgt cgttcgacga ggaggcgcgc gagctgacct tgcgcgcggc ggagcaggcg
     5881 ggcctgaagc gcgtgacgct tttggaagag ccgcaggccg cgttctacgc gtggatcgac
     5941 gcgcagggcg aaagctggcg caagcgcatc aaggtcggcg atttgatcct cgtctgcgac
     6001 gtggggggcg gcacgaccga cttcagcctg attacggtct cggaagagaa cggcgagctg
     6061 tcgctcaagc gcgtcgcggt cggcgagcac atcctgctcg gcggcgacaa catggacttg
     6121 gcgctcgcgc ggctgctgca acagcggctc gaagaggaag gccatcgcgt tgacacttgg
     6181 cagttgcagg gcttgtggca ccagtgccgg ctggcgaaag agaaactgtt cgcggagccg
     6241 aagagccaga agcagccggt gacgctgttg ggcaaaggct cgaagctcgt cggcggcacg
     6301 atcaagaccg agctgcggcg cgaagacctc gaacgcgtgt tgacggacgg tttctttccc
     6361 gtcgtcgggc gcgaagagat gcccgcgaag caaaggcgca tcggctttca ggaactcggg
     6421 ctgccttacg ccgcggacgc cgccgtgacg aaacacctcg cgcgcttcct gtcgcagcag
     6481 acggcggcga acgacgccga gggcgaccgg attcggcgcg gcccgagcgg cttcgcctgt
     6541 ccgacgcacg tcctcttcaa cggcggcgtg atgaaggccg cgatgctgcg cgagcgcgtc
     6601 gtgcaggttc ttgacgagtg ggtgcgggcc gagggcttcg actctttggg cgagcgcggc
     6661 gttttggaag cgcccgacct cgaccacgcg gtcgcgcgcg gggcggccta ctacggcaag
     6721 gccaagcgcg ggcgcggggt gcgcatccgc agcggcgcgc cgcgcaccta ctacatcggc
     6781 atcgagagcg cgatgcccgc cgtgccgggg atgcccgcgc ccttgaaggc gctctgcgtc
     6841 gtgcccttcg gcatggagga ggggactgaa ggccagatac cgggccgtga gttcggcctc
     6901 gtcgtcggcg aacccgccga gttccgtttc ctcagctcga ccacgcgcag gca
//
LOCUS       DBNGOH010000638         5354 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3576116, whole genome shotgun sequence.
ACCESSION   DBNGOH010000638 DBNGOH010000000
VERSION     DBNGOH010000638.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5354)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5354)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5354
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3576116"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /locus_tag="AC159P_12500"
     CDS             complement(<1..257)
                     /locus_tag="AC159P_12500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009838410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HIR3696312.1"
                     /translation="MKILFLVALVLLSLPLLGLAQQGQRKLFPYDYTIDDLPNGLRLI
                     TVPTDYPNLVSVYIVVAAGSRNEVEQGKSGFAHFFEHMMFRG"
     gene            complement(345..1133)
                     /locus_tag="AC159P_12505"
     CDS             complement(345..1133)
                     /locus_tag="AC159P_12505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696313.1"
                     /translation="MKKLCLSIVLLASLSHAATAQEAAQGGARKIDECGDIQISDWLA
                     RADNFAIELQNAPAAKGYIVAYGVPNKFPGWPLRRAYQIKGYLIAGRGLQAERVEVVN
                     GGYRDAVMFQLWVVESGAQLPLPPFDLGAALSRERTPLLFDRFYPHDTQPSTDIDAGY
                     EGYLDAIKARFEPFAQALRSDPAARGCVIVYSSHRDRRGADRRLAARQKRQILTTHSL
                     DPARVAALAGGRRNSRAVELWIVPPGSPLPKPTPTARPARRRRR"
     gene            complement(1186..2637)
                     /locus_tag="AC159P_12510"
     CDS             complement(1186..2637)
                     /locus_tag="AC159P_12510"
                     /inference="COORDINATES: protein motif:HMM:NF016021.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696314.1"
                     /translation="MLNIERSHATMGDASYKEASRLRGSALSETEATFYVTQRKLAFA
                     PCLGHERLLRSLLDMRVDRPRLRFLEQDRGGLALFAKAVEDINFAGVVRAVRPGTIVF
                     AGQPFADITGAFGLTQAQEIKFEHAFDLPMTTAALAMQMRMAAGRRWLSDFSLRRNGD
                     VERAVEVATYAFIGGFNDTSNMEAAHRLDIPAVGTAAHYWQQSFIEFMDEPEIEPRTG
                     RPKHFEQVAFERWLDTNPQGTTLLLDTIDVRMGAVHAAMAATSTEARRAAFKGFRVDS
                     GDLAELGKWCLDFFEANGLTGLMPVLTGDLDAEGIRQIVAAFPEAAGFGIGTKLSGEV
                     RRIAGVIFKECVVGGRPTLKASNTPEKSTLPGRLQVFRGIDEAGRYVGDVTGLADEQL
                     DIPGAARVQPLLAPFWEGGRHEPIPSITKQKAFVEEQLARFDDINAYPRTLSDKLRTL
                     RDELTARMREDESNWRAVLRVPDALAASLEGTT"
     gene            complement(2745..3314)
                     /locus_tag="AC159P_12515"
     CDS             complement(2745..3314)
                     /locus_tag="AC159P_12515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012648504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamidase"
                     /protein_id="HIR3696315.1"
                     /translation="MSMDVDTNGGGRALIVVDVQNDFCPGGALAVERGDEVVAPLNRL
                     IEEFLGRGEPVYKTRDWHPPETKHFAAYGGTWPVHCVRNTRGAEFHPDLSDDPRIRVV
                     SKGLGDEDCYSAFDGTDLADELRAKNVGEVWVGGLATDYCVKNTVLDARREGFRVRAL
                     RDAMRAVNLQPGDDRQAVAEMRDAGAEIV"
     gene            3464..>5354
                     /locus_tag="AC159P_12520"
     CDS             3464..>5354
                     /locus_tag="AC159P_12520"
                     /inference="COORDINATES: protein motif:HMM:NF024753.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB
                     family protein"
                     /protein_id="HIR3696316.1"
                     /translation="MEKNRFRKNQLQKSAACFSLVLWCALAPQVFAGARPNAAAARDA
                     APRWSAKLDGEVRFYLPTEMGVLVAGTERSLYAVDAETGEIVWRRKDARLDESDVAPV
                     PGTDLLLLSFERGDKTRLEAVDVLTGGRVWQSEKVRGAPMHLAVDADARLLAAVFARD
                     ARGRAREGFKKRPVVHVFDLGTGEERWERKLDSEVEMLPARWPEKEDADVAYSLDNYR
                     APLFLDGRLYLFYEGITSLDAATGEERRREKFRVNEEGLALTDADPVFDEESIYASGR
                     GRVRAISRATGEEVWEAKDLGLTPELIPVGDVLYVRTGGQFTRLKDGEIDERGPYGVS
                     ALDRRTGKTLWRYKGADKGITNLALPDAATILIADRDDLISIDTRTGKRRAKIEHGVE
                     RAAFVLLNEAGLAVVGGRNAIAGLDAATGRVAWRAQHNPPGRGILRTVTAVAARAAAL
                     YFRYGGAATTVFRGAQVARGISGLRTGLRFRAALPSLQALAADTARDYVTQRVTTFGA
                     ASQLGQLGDAAAVARRAREVQNLRTPRVGVSAPSIDVEERLLDRLDPARQLERLSAYL
                     LRRRRLAALRGQWMYFYTNLPGGGGRGLAGVNVNNGRADRAIPLGDPDERFLVDEVGG
                     LLHTAR"
BASE COUNT          832 a         1727 c         1951 g          844 t
ORIGIN      
        1 ccgcggaaca tcatgtgctc gaagaagtgg gcgaagccgc tcttgccctg ctcgacctcg
       61 ttgcgcgagc cggcggcgac gacgatgtag accgagacga ggttcgggta gtcggtcggc
      121 acggtgatca gccgcaggcc gttcggcagg tcgtcaatcg tgtagtcgta cgggaagagc
      181 ttgcgctgcc cctgctgcgc gaggccgagg aggggcagcg agagcagcac gagagcgacg
      241 agaaacagga ttttcatatc gctgtggact ccgcgattag gttcgaaatt tagtttggag
      301 acgcggcgat tatacacgag cggggccgca cagctcattc gtgcctatcg cctccggcgg
      361 cgcgcggggc gcgcggtcgg ggtcggcttc ggcagcggcg agccgggcgg gacgatccac
      421 aactcgacgg ccctgctgtt gcgcctgccg cccgcgagcg ccgcgacgcg ggccgggtcg
      481 agcgagtggg tcgttaaaat ttgacgcttc tgccgcgcgg cgagccgccg gtcggcgccc
      541 cgccggtctc tgtgcgagga gtagacgatg acgcagccgc gcgccgccgg gtcgctgcgg
      601 agcgcctgcg cgaaaggctc gaagcgcgcc ttgatcgcgt cgagataacc ttcgtacccg
      661 gcatcgatgt ccgtagaggg ctgcgtatcg tgggggtaga agcggtcgaa gagcagcggc
      721 gttctttccc tcgaaagcgc cgccccgagg tcgaacggcg ggaggggcag ttgcgcccca
      781 ctctcgacca cccacaattg gaacatgacc gcgtcgcggt agccgccgtt gaccacctcg
      841 acgcgctcgg cctgaagccc cctccccgcg atcaaataac ccttgatctg gtaggcgcgg
      901 cggagcggcc agcccggaaa cttgttcggc acgccgtagg cgacgatgta gcccttcgcc
      961 gcgggcgcgt tctgcaactc gatggcgaag ttgtcggcgc gcgccagcca gtccgagatt
     1021 tggatgtcgc cgcactcgtc gatcttgcgc gcgccgccct gcgccgcttc ctgcgccgtg
     1081 gcggcgtgcg aaagcgaggc gagcaggacg atagacaggc agagtttttt catcggggat
     1141 gattccatgc cgccggagcg ggcgcgggca ttatagccga acccttcaag tcgtcccttc
     1201 gagcgaggcg gcgagcgcgt cgggcacgcg gaggactgcg cgccagttcg attcatcttc
     1261 gcgcatccgc gcggtcagct cgtcgcgcag cgttcgcagc ttatcgctca gcgtgcgcgg
     1321 gtaggcgttg atgtcgtcga agcgggcgag ctgttcttcg acgaaggctt tctgcttggt
     1381 gatggagggg atcggctcgt ggcgtccgcc ctcccagaag ggggcgagca gcggctggac
     1441 gcgcgcggca ccggggatgt cgagctgttc gtcggcgagt ccggtcacgt cgccgacgta
     1501 gcggcccgct tcgtcgatgc cgcggaagac ctgaaggcga ccggggagcg tggacttctc
     1561 cggcgtgttc gaagccttga gcgtggggcg cccgccgacg acgcactcct tgaagatgac
     1621 gcccgcgatg cggcgcacct cgcccgagag cttggtgccg atgccgaagc ccgcggcctc
     1681 ggggaacgcg gcgacgattt gccgaatccc ttcggcgtcg aggtcgccgg tgaggacggg
     1741 catgaggccc gtcagcccgt tggcctcgaa gaagtcgagg caccacttgc ccagctccgc
     1801 gaggtcgccc gaatcgacgc ggaagccttt gaaggccgcg cgccgcgcct ccgtggaggt
     1861 ggcggccatc gccgcgtgga cggcgcccat gcgcacgtcg atggtgtcca ggaggagcgt
     1921 ggtgccctgc gggttggtgt cgagccagcg ctcgaaggcg acctgctcga agtgcttggg
     1981 gcggcccgtg cgcggctcga tctccggctc gtccatgaac tcgatgaagg actgctgcca
     2041 gtagtgcgcg gcggtgccga ccgcgggaat gtcgaggcgg tgcgcggcct ccatgttcga
     2101 ggtgtcgttg aagccgccga tgaaggcgta ggtggcgacc tcgacggcgc gctccacgtc
     2161 gccgttgcgg cgcagtgaga agtccgagag ccagcgacgc ccggcggcca tccgcatctg
     2221 catggcgagc gcggcggtcg tcatcggcag gtcgaaggcg tgctcgaact tgatctcctg
     2281 cgcctgcgtc aaaccgaacg cgcccgtgat gtccgcgaac ggctggcccg cgaagacgat
     2341 ggtgccgggc cgcaccgcgc gcaccacgcc ggcgaagttg atgtcctcga ccgctttggc
     2401 gaagagcgcg aggccgccgc gatcctgttc gaggaaccgc agccgtgggc ggtcaacgcg
     2461 catgtcgagc agcgagcgca atagccgctc gtggccgagg cacggcgcga acgcgagctt
     2521 gcgctgcgtc acgtaaaagg tcgcctccgt ctcgctcagt gcagagccgc gcagccgcga
     2581 cgcctccttg tacgaggcgt cgcccatcgt ggcgtgggaa cgctcaatgt tcagcataaa
     2641 agggaatgat gaatgcagag tgatgaatga tgaataaaag accgcttgaa tgataaaagg
     2701 caacacgcct ttcaaacatc attccgcact ctgcattcat catttcagac tatttccgcc
     2761 ccggcgtcgc gcatctccgc gacggcctgc cggtcgtcgc cgggttggag gttgacggcg
     2821 cgcatggcgt cgcggagggc gcggacgcgg aagccttcgc ggcgggcgtc gaggaccgtg
     2881 ttcttgacgc agtagtccgt ggcgaggccg ccgacccaga cttcgccgac gttcttcgcg
     2941 cgcagctcgt cggccagatc ggtgccgtcg aaggccgagt agcagtcttc gtcgccgaga
     3001 cctttggaga cgacgcggat gcgcgggtca tccgagaggt cggggtggaa ctcggcgccg
     3061 cgcgtgttcc gcacgcagtg gacgggccag gtgccgccgt aggcggcgaa gtgtttggtc
     3121 tcgggcgggt gccagtcgcg cgtcttgtag acgggttcgc cgcgtccgag gaactcttcg
     3181 atcaagcggt tgagcggcgc gaccacctcg tcgccgcgct cgacggcgag cgcgccgccg
     3241 gggcagaaat cgttctgcac atcgacgacg atcagcgccc gtccgccgcc gttcgtgtct
     3301 acgtccatgc tcatcacacc ggccctcccg ggaagcgcgc gcggcgtccg ttaaaaagcc
     3361 ggcggcctgc tttgtaacga agtgtcgcag ccgttaaact ggccttagct tacctctcct
     3421 cggaaggagg cggcaacgcg cgctccccgc tttgcggctc gtgatggaaa aaaatcggtt
     3481 tcgcaaaaat caactgcaaa agtcggcggc ctgtttctcg ctcgtgctct ggtgcgcgct
     3541 cgcgccgcag gtcttcgcgg gcgcccgtcc gaacgcggcg gcggcgaggg acgcggcgcc
     3601 gcgttggtct gcgaagctcg acggcgaggt gcgtttctac ctgccgacgg agatgggcgt
     3661 gcttgtcgcg gggacggagc gttcgctgta cgcggtcgat gcggagacgg gcgagatagt
     3721 gtggcggcgc aaggacgcgc ggctcgacga gtcggacgtg gcgccggtgc ccggcacaga
     3781 ccttctgctt ttgagcttcg agcggggcga caagacgcgg ctcgaagccg tggacgtgtt
     3841 gacgggcggg cgcgtctggc agagcgagaa ggtgcgcggc gcgccgatgc acctcgcggt
     3901 cgatgcggac gcgcggctgc tcgccgcggt cttcgcgcgc gacgcgcgag ggcgtgcgcg
     3961 cgagggcttc aagaagcggc ccgtcgtgca tgtcttcgac ctcggcaccg gtgaagagcg
     4021 ttgggagcgc aagctcgaca gcgaagtcga gatgctgccc gcgcgctggc ccgagaaaga
     4081 ggacgccgat gtcgcgtact cgctcgacaa ctaccgcgcg ccgctcttcc tcgacggtcg
     4141 cctctacctg ttctacgaag gcatcacttc gctcgacgcg gcgacgggtg aggagcggcg
     4201 gcgcgagaag ttccgcgtca acgaagaggg cctcgcgctg acggacgccg accccgtgtt
     4261 cgacgaggag tcgatctacg cctccgggcg cgggcgcgtg cgcgccatct cgcgcgcgac
     4321 gggcgaagag gtgtgggagg cgaaagacct cgggctgacg ccggaactga tccccgtggg
     4381 cgacgtgctc tacgtgcgca cgggcgggca gttcacacgg ctgaaggacg gcgaaataga
     4441 cgagcgcggg ccttacggcg tgagcgcgtt agaccggcgg acgggcaaaa ccctgtggcg
     4501 ctacaagggc gcggacaaag gcatcacgaa cctcgcgctg ccggacgcgg cgacgattct
     4561 catcgccgac cgcgatgacc tcatctcgat cgacacacgg acgggcaaac ggcgcgcgaa
     4621 gatcgagcac ggcgtcgagc gcgcggcgtt cgtgctgttg aacgaggcgg gcctcgccgt
     4681 ggtcggcggt cgcaacgcga ttgcgggcct cgacgccgcc acggggcgcg tcgcgtggcg
     4741 cgcgcagcac aacccgccgg gccgcggcat cctgcgcacc gtcacggcgg tcgcggcgcg
     4801 cgcggcggcg ctctacttcc gctacggcgg cgcggcgacg accgtcttcc gcggcgcgca
     4861 ggtcgcgcgc gggataagcg gcttgcggac gggcctgcgt ttccgcgccg cgctgccttc
     4921 gctacaggcg ctcgccgccg acacggcgcg cgactacgtg acgcagcgcg tgacgacctt
     4981 cggcgcggcg tcgcagcttg ggcagctcgg cgacgcggcg gcggtcgcgc ggcgcgcgcg
     5041 cgaggtgcag aacctgcgga cgccgcgcgt cggcgtcagc gccccttcaa tcgatgtcga
     5101 ggagcgtctg ctcgaccgtc tcgacccggc gcggcagctc gaacgtttgt cggcatacct
     5161 gctgcgccgg cggaggctcg cggcgctgcg cgggcagtgg atgtacttct acacgaacct
     5221 gccgggcggg ggcgggcgcg gcctcgcggg cgtcaacgtc aacaacggtc gcgccgaccg
     5281 cgccatcccg ctcggcgacc ccgacgagcg tttcctcgtg gacgaagtcg gcggcctgct
     5341 ccacaccgcg cgcg
//
LOCUS       DBNGOH010000639         2316 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_5043173, whole genome shotgun sequence.
ACCESSION   DBNGOH010000639 DBNGOH010000000
VERSION     DBNGOH010000639.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2316)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2316)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2316
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_5043173"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="AC159P_12525"
     CDS             <1..213
                     /locus_tag="AC159P_12525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HIR3696317.1"
                     /translation="GTRREEYPRAYTRMEVRHVVRGHNVSEKAVASAIELSETKYCSV
                     AATLRAGVEIVTSYEIIEETGGAAGD"
     gene            371..1864
                     /locus_tag="AC159P_12530"
     CDS             371..1864
                     /locus_tag="AC159P_12530"
                     /inference="COORDINATES: protein motif:HMM:NF013618.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008933 - peptidoglycan lytic
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HIR3696318.1"
                     /translation="MIALAHTFSVCLTALALSLACARVENAPAANDADGAALPYNPEA
                     IVLSAEELSQDALSATRRMDSSGRGADGQLQKLPAQEHLRRAAIYHANRAFDEARAHW
                     RAVIAYYPEDSNVPNAHFLVGRSLFQEKRYEEALPIFGWLGDNYAQTQGGSEGFYYVA
                     ATLLRLNRPGEAAARYAEYIERYPQGERVEAAHLNIIDSLREAGRPNDALPWIDRTRE
                     RYRGQPADTNALFARLRLDVARKDWASALRTADELSRASLGRNVGTSQSEVAYLRAYV
                     LEHSGQKEQASRIYQGIADSLGSYYGSRATARLEKIGGASKSAAAAREARVHSEAKRL
                     AGDYPAPYREQILRAVKGRDVDPRLMLAIMRQESGFKPGAKSPAGARGLMQFTPDVAA
                     KYAPQVKLGKLSEEDLHRPEVSILLASAYLDELAKMFPGLPEAVAASYNGGEDNVARW
                     VRRAAHDDRGVFTSEIGFTESKDYAMKVAANLRAYTELYTDDLKPRR"
     gene            1966..>2316
                     /locus_tag="AC159P_12535"
     CDS             1966..>2316
                     /locus_tag="AC159P_12535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HIR3696319.1"
                     /translation="MSIIAFESLLIILLVVVNGVFAMSELAVVSSRKGRLQQLANAGD
                     VGARAALELANNPDRFLSTVQIGITLVGILAGAFGGATIAEQIGFSLGGVPALAPYGE
                     AIGLGIVVLAITYLS"
BASE COUNT          410 a          788 c          744 g          374 t
ORIGIN      
        1 ggcacgcgcc gcgaggagta cccgcgcgcc tacacgcgga tggaggtgcg ccacgtggtg
       61 cgcggccaca acgtctccga aaaggcggtc gcctcggcca tcgagctttc ggagacgaag
      121 tattgcagcg tcgcggcgac gctgcgcgcg ggcgtcgaga tcgtgacgag ctacgagatc
      181 atcgaagaga ccggcggggc ggcgggcgac tgagcaagag gtatcttcgg acgaacgggc
      241 gcgagccttc ccttcgtctt cgttaacgcg ccggacattt ttacaggcac tcgtctttct
      301 cgctccccgc atcgaacacg agagagcttc gcccttgcga acaccgaacg aaggacgaca
      361 cggattgagc atgatcgcac tcgcacacac cttctccgtc tgcctgacgg cgctcgcgct
      421 ctcgctcgcc tgcgcgcgcg tcgagaacgc cccggcggcg aacgacgccg acggcgcggc
      481 gctcccgtac aacccggagg cgattgtcct ctccgcagag gagctgtcgc aggacgcgct
      541 ctccgcgacg cggcggatgg actcttcggg ccgcggcgcc gacgggcagc tacagaagct
      601 cccggcgcag gagcacctgc gccgcgccgc catctaccac gccaatcgcg ccttcgacga
      661 agcgcgcgcg cactggcggg cggtcatcgc ttattacccc gaagactcga acgtgccgaa
      721 cgcgcatttc ctcgtcggtc gctcgctctt tcaggagaag cgctacgaag aggcgctgcc
      781 catcttcggc tggctcggag acaactacgc gcagacgcag ggcgggagcg agggcttcta
      841 ctacgtcgcc gcgacgctgc tgcggctgaa ccgaccgggc gaggccgccg cgcgctacgc
      901 cgaatacatc gagcgctacc cgcagggcga gcgcgtcgag gccgcgcacc tcaacatcat
      961 cgactctctg cgcgaggccg ggcgccccaa cgacgcgctg ccgtggatcg accgcacgcg
     1021 cgagcgctac cgcggacagc ccgccgacac caacgccttg ttcgcacgcc tgcgtttgga
     1081 tgtagcgcgc aaagactggg cgagcgcgct ccgcacggcg gacgagctga gccgcgcgag
     1141 cctcggtcgc aacgtcggca catcgcagtc ggaggtcgcg tacctgcgcg cctacgtgct
     1201 cgaacattcg gggcagaagg agcaggcgag cagaatctat caggggattg cggacagcct
     1261 cggctcttac tacgggtcgc gcgcgacggc gcggctcgaa aagattggcg gcgcgtcgaa
     1321 gagtgccgcc gcggcgcgcg aggcacgcgt ccactcggag gcgaagcggc tcgcgggcga
     1381 ctatcccgcg ccctaccgcg aacaaatcct gcgcgccgtc aaagggcgcg acgttgaccc
     1441 gcgcctgatg ctcgccatca tgcggcagga gagcggcttc aagccgggcg ccaagtcgcc
     1501 cgcgggcgcc cgcggcctga tgcagttcac gcccgacgtg gccgccaagt acgcgccgca
     1561 ggtcaagctc ggcaaactgt cggaggaaga cctccaccgc cccgaagtct ccatcctgct
     1621 ggcgagcgcg tatctagacg agctggcgaa gatgtttccg ggcctgcccg aagcggtcgc
     1681 ggcctcctac aacggcggcg aagataacgt cgcacgttgg gtgcgccgcg ccgcccacga
     1741 cgaccgcggc gtcttcacct ccgagatagg cttcacggag tccaaagact acgccatgaa
     1801 agtcgccgcc aacctgcgcg cctacacgga gctttacacg gacgacctca agccccgccg
     1861 gtaacttgta gttcggacag ctcccgcttt cggctcttga cgacaggtag ccatggttta
     1921 tccttgaagt ccgacttttt ttggtagtcg ggctgagagg aaatcatgtc catcatcgca
     1981 ttcgaatctt tacttatcat tttacttgtc gtcgtcaatg gcgttttcgc gatgtccgag
     2041 ctggcagtcg tgtcttcgcg gaaagggcgg ctgcaacaat tagccaacgc cggtgatgtg
     2101 ggagcgcgcg ccgcgctgga gctggccaac aatcccgacc gtttcctgtc aaccgttcaa
     2161 atcggtatca cgctcgtcgg cattctggcc ggcgccttcg gcggcgcgac catcgccgag
     2221 caaatcggat tcagcctcgg cggcgttccg gcgctcgcgc cgtacggcga agctatcggg
     2281 ctgggcatcg tggtcttggc catcacctac ctttcg
//
LOCUS       DBNGOH010000640         6442 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1195647, whole genome shotgun sequence.
ACCESSION   DBNGOH010000640 DBNGOH010000000
VERSION     DBNGOH010000640.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6442)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6442)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6442
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1195647"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            77..697
                     /locus_tag="AC159P_12540"
     CDS             77..697
                     /locus_tag="AC159P_12540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696320.1"
                     /translation="MAVVHPFKQHTAAEEPPALHDRAMDNLRFIRETMERATAFTAVP
                     GWGQVAIGVTAIFAALVASQQRSTEAWLAIWMAEAVVALVIAGWTMYRKARSSATPLL
                     SQPGRKLAINFSPPFFVAVLLTIVFYRAGLAGLLPGLWLLLYGTGVVTGGSFSVRPVP
                     FMGLCFMFVGAGAFFCPPAWGDALMAAGFGALHIVFGIHIARRNGG"
     gene            697..1119
                     /locus_tag="AC159P_12545"
     CDS             697..1119
                     /locus_tag="AC159P_12545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HIR3696321.1"
                     /translation="MARQSTLAKEREKAKGRGGAPHQQGPLRDVRRAKSHEPAAPPSA
                     AELDRLIHERVRLAIVSALAVNDSLTFNELKELLNTTDGNLSVHARKLEEAQYVTCNK
                     FFDGRTPKTEYRLTATGRRALESYLNHMESVIRAARGR"
     gene            1234..1971
                     /locus_tag="AC159P_12550"
     CDS             1234..1971
                     /locus_tag="AC159P_12550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696322.1"
                     /translation="MEQVYRFFVENTPPPGVIVKGGPVFVAWAYLCLRLSAHLKCARG
                     VKTGYTRKTFHVLTFLSAVCIQLVWGLPVLCLYGAAVSCVLAYAVARGAGHPLYEAIA
                     REQDAPHRTYYVVVPYFATLVGGVLSNVLFGHAAIIGYLVGGLGDAAGEPVGTRWGRH
                     RYRVPSFGGVRVTRSIEGSAGVMLFSLVAILVGVGLSPELQLTPSNAWLPLPLAACCA
                     LLEAASPHGWDNASMQLVPSFMAATLL"
     gene            2011..3165
                     /locus_tag="AC159P_12555"
     CDS             2011..3165
                     /locus_tag="AC159P_12555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012891717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR3696323.1"
                     /translation="MEQFDVVVVGAGLAGLQATRLLARRGVRVLLVDRKASLADSVHT
                     TGIFVRRTLEDFRLPESCLGRPVRHVTLYSPARRALTLRSSHDEFRVGRMARLYEHFL
                     EDALGAGAAWSPATRYAWLEDDGQRGSVVRLERGGRARDVRARFVVAADGALSRVASD
                     LGLDANSEWIVGVEDVYEGVPLEGEPRFHCFLDPRLAPGYLAWVVHDGEEAHVGVGGY
                     AARFDPLKALEVFRASLASLFDFSRSRKVERRGGRIPVGGVLKRIANARGLLVGDAAG
                     AVSPLTAGGLDPCMRLSSLAAEVAADYVKSEDAAALARYSGEQFRARFVSRLWMRRVL
                     RATDHPALVEGACALMRLPPFNAVAWQVFFGRGSFPDVKTETVCGYSTAT"
     gene            3191..3622
                     /locus_tag="AC159P_12560"
     CDS             3191..3622
                     /locus_tag="AC159P_12560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696324.1"
                     /translation="MKENARRIRRPTKHVEILAVLHVVYGGFHFLAFGPMFALSLGVL
                     LTQGQRAWENEFVSDGITACVIGIPTLVGMPFAAAYFLSQRRRCAKVFVALSAVFALA
                     LALFLAYISLAESEAGWAIYFAPCLALSGYSLWFVCRRPFL"
     gene            complement(3639..3989)
                     /locus_tag="AC159P_12565"
     CDS             complement(3639..3989)
                     /locus_tag="AC159P_12565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012647139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="avidin-fold protein"
                     /protein_id="HIR3696325.1"
                     /translation="MGAFDYDERFFAPVSNSATGEVGSETVFHYRQRGEVVWATYEGS
                     EVAFGTLTAKVLEDGSLDMRYAHVNRRGELMTGRCRSTPELLPDGRLRLHERWQWTSG
                     DHSAGESVVEELKA"
     gene            complement(4003..4584)
                     /locus_tag="AC159P_12570"
     CDS             complement(4003..4584)
                     /locus_tag="AC159P_12570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696326.1"
                     /translation="MNIFVTRPLILLLLAAVSVAAGASQAAAQVLPPLPPQLTQRQQT
                     PQQAAAAKDDGSADDNDDDDDDADNATQTRASDPAGLLRTARSVYVRSNTVFVKAAEV
                     EDSLRKRKEFRAWGMTVTRNLADADLVIEIERKSLTRRFTFTVLDPHTLAVLTSGKTR
                     SVLFGKKIHNKVAEKFANRVKTVRPYPPVSSNP"
     gene            4932..6371
                     /locus_tag="AC159P_12575"
     CDS             4932..6371
                     /locus_tag="AC159P_12575"
                     /inference="COORDINATES: protein motif:HMM:NF013594.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HIR3696327.1"
                     /translation="MSRLTIWRLLLITFLCAQLACGGLLSGDSGSDVDDAGGARPAPH
                     FEPGFNLFSPEQDIELGRMSAREIAQQVPVMTDARTTQYVQQLGAKLAAHAPGFKFPY
                     QFAVVGTREINAFALPGGPIFVNAGTIAAAKNEGELAGVIAHEITHIALRHGTNQASK
                     AYIAQKGLGILSEIVGGDGGDLGSIVSAIGGAGANMIFLKFGRTAETQADLEGARLLA
                     AAGYDPRDMATFFEKLQSQSGGQRVPEMLSDHPDPGNRIAAINQVMPSLRVSQKPVRD
                     SNEFQQVKARLTGGAVKSEAEPQRVGPRDPTDIEPGARPPAPSSEFREFQSKDGSFAL
                     QYPANWDAIGAPEGDANLIFAPKGAYGKMGDSLYVSHGIFIGVVAPQAGDLAAANEAF
                     VRQQLAANPDFRVQRNPERIESGGRQWLRTLVAGPSAVTGVIEIDVIYTSATSDGRLF
                     YLITMAPEDEFQNYQPAFERIIGSLRLSR"
BASE COUNT          943 a         2137 c         2257 g         1105 t
ORIGIN      
        1 acccgcggtt ccgccgtttg ctttgcactg taaagcactt gacaaaggcg ccgccgcgtc
       61 gtatcttgca cgcggcatgg cagtcgtcca cccgttcaaa cagcacaccg ccgccgagga
      121 gccgcccgcg ctgcacgacc gcgcgatgga caacctgcgc ttcatccgcg agacgatgga
      181 gcgcgccacg gcttttaccg ccgtgcccgg ctgggggcag gtcgccatcg gcgtgacggc
      241 catcttcgcg gcgctcgtcg cgtcgcagca gcgctccacg gaggcgtggc tcgccatctg
      301 gatggcggag gccgtggtcg cgctcgtcat cgccgggtgg acgatgtacc ggaaggcgcg
      361 cagcagcgcg acgccgctgc tctcgcagcc cggcaggaag ctggcgatca acttctcgcc
      421 gccgttcttc gtcgccgtgc tcctgacaat cgtcttctac cgcgccggtc tcgccggtct
      481 gctgcccggc ctgtggctcc tgctctacgg gacgggcgtc gtcacgggcg gctcgttttc
      541 ggtgcgcccg gttcccttca tggggctgtg cttcatgttc gtgggggcgg gcgccttctt
      601 ctgcccgccc gcctggggcg acgcgctgat ggccgcgggt ttcggcgcgc tgcacatcgt
      661 cttcggcata cacatcgcga ggaggaatgg aggatagtgg ccagacaaag cacgcttgcc
      721 aaagagaggg agaaggcgaa gggtcgcggc ggggcgccgc atcaacaggg gccgctcagg
      781 gacgtgcggc gcgcgaaatc gcacgaaccc gcggcgccgc cgtccgccgc cgaactcgac
      841 cgcctcatcc acgaacgcgt ccgcctcgcc atcgtcagcg ccctcgccgt caacgactcg
      901 ctcaccttca acgaactcaa agaactcctg aacacgaccg acggcaacct gagcgtccac
      961 gcgcggaagc tcgaagaggc gcagtacgtc acctgcaata agttcttcga cgggcggacg
     1021 ccgaagaccg aatacaggct gaccgccacg ggccgcaggg ccttggaaag ctacctcaac
     1081 cacatggagt cggtcatccg cgcggcgcgc gggagatgaa gcaggggcca cgacggcccg
     1141 atttttttgc ccgcttgctt tacggcgcaa aggtctttat tcgggccggc tgacgaaggc
     1201 ggggagaaac gcgtcggggc gttcaagcgg ggtatggagc aggtctacag gttcttcgtc
     1261 gagaacacgc cgccgccggg cgtcatcgtc aagggcgggc ctgtcttcgt cgcgtgggcg
     1321 tacctatgct tgcgcctctc ggcgcacctg aagtgcgcgc gcggcgtgaa gaccgggtac
     1381 acgcgcaaga ccttccacgt gctcaccttt ctttccgccg tctgcattca gctcgtgtgg
     1441 gggctgccgg tcttgtgcct gtacggcgcg gcggtctcgt gcgtgctcgc ctacgcggtc
     1501 gcgcgcggcg cgggccaccc gctgtacgag gcgatagcgc gcgagcagga cgcgccgcac
     1561 aggacttact acgtcgtcgt gccttacttc gccacgctcg tcggaggggt cttgagcaac
     1621 gtgctgttcg gccacgcggc gatcatcggc tatctggtgg gcgggctggg cgacgccgcg
     1681 ggcgagccgg tcgggacgcg ttgggggcgg caccgctacc gagtcccttc gttcggcggg
     1741 gtgcgtgtga cgcggagcat cgaaggctct gcgggcgtga tgctcttctc gctcgtcgcc
     1801 atcctcgtcg gcgtcgggtt gagtcccgag ctgcaactca cgccgagcaa cgcgtggctg
     1861 ccgttgccgc tggcggcgtg ctgcgcgctg cttgaggcgg cctcgccgca cggttgggac
     1921 aacgcctcga tgcagctcgt gccgtcgttc atggcggcca cgttgttgtg agctttgaat
     1981 cgtgagcgcg tgaccgcgga gaaatgtcat atggaacagt ttgacgtggt ggtggtgggc
     2041 gcggggctgg ccgggctaca ggcgacgcgg ctgttggcgc ggcgcggcgt gcgcgtcctg
     2101 ctggttgacc gcaaggcttc gctcgccgac tcggttcaca cgacgggcat cttcgtccgc
     2161 cgcacgctcg aagacttccg cctgccggag agttgtttgg ggcggcccgt gcggcacgtc
     2221 acgctctact cgcccgcgcg gcgcgcgctc acgcttcgta gctcgcacga cgagttccgc
     2281 gtcgggcgca tggcgcggct ctacgagcac ttcctcgaag acgctctcgg cgcgggcgcg
     2341 gcgtggtcgc cggccacgcg ctacgcgtgg ctcgaagacg acggccagcg cggctcggtc
     2401 gtgcgtctgg aacggggcgg tcgtgcgcgt gacgtgcgcg cgcgtttcgt cgtcgcggcg
     2461 gacggcgcgc tctcgcgcgt ggcgagcgac ctcgggttgg acgccaacag cgagtggatc
     2521 gtcggcgtcg aggacgtgta cgaaggcgtg ccgctcgaag gcgagccgcg cttccactgc
     2581 ttcctcgacc cgcgcctcgc gcccggctac ctcgcgtggg tcgtgcacga cggcgaggag
     2641 gcgcacgtcg gcgtcggcgg ctacgccgcg cgcttcgacc ctctgaaggc gttggaggtt
     2701 ttccgcgcga gcctcgcgtc gctgttcgac ttcagccgct cgcgcaaggt cgaacgccgc
     2761 ggcgggcgca tcccggtggg cggcgtgctg aagcgcatcg ccaacgcgcg cggcctgctg
     2821 gtcggcgacg cggcgggcgc cgtctcgccg ctcacggcgg gcggcctcga cccgtgcatg
     2881 cgtctgtcga gcctcgcggc ggaggtcgcc gccgactacg tgaagagcga agacgcggcg
     2941 gcgctcgcgc gctactcggg cgagcagttc cgcgcgcgct tcgtctcgcg gctctggatg
     3001 cggagggtct tgcgcgcgac cgatcacccg gcgctcgtcg aaggggcgtg cgcgctgatg
     3061 cggctgccgc ccttcaacgc cgtcgcgtgg caggtcttct tcgggcgcgg ctcgttcccg
     3121 gacgtgaaga cggagacggt gtgcggatat tcgaccgcga cttagacggc gggcgccgcg
     3181 gagggtgagg atgaaagaga acgcacgtcg aattcgcagg ccgacgaagc acgtggagat
     3241 tctggcggtg ctccacgtcg tctacggcgg ctttcacttt ctggcgttcg ggccgatgtt
     3301 cgcgttgtct ctcggcgtct tgctcacgca ggggcagcgc gcgtgggaga acgaatttgt
     3361 gagcgacggc atcacggcct gcgtgatcgg catcccgacg ctcgtcggga tgccttttgc
     3421 cgccgcctac tttctgtcgc aacggcgccg ctgcgccaaa gtcttcgtgg ccctctccgc
     3481 cgtgttcgcg ctcgcgctcg cgctttttct ggcgtacatc agcctcgcgg agtcggaggc
     3541 tggctgggcc atctacttcg cgccgtgtct ggcgttgagc ggctactctt tgtggttcgt
     3601 ctgccgcagg ccgttcctct gaagcgacgc cgcgagactc acgccttcaa ctcttcgacg
     3661 acggactcgc ccgcggagtg atcgccgctc gtccactgcc aacgttcgtg caggcggagg
     3721 cgaccgtcgg gcagcagctc cggcgtcgag cggcagcgcc ccgtcatcaa ctcgccgcgg
     3781 cggttcacgt gcgcgtagcg catgtcgagc gagccgtctt ccagcacttt agctgtcaac
     3841 gtgccgaacg cgacctcgct gccctcgtac gtcgcccaca ccacttcgcc cctctgccgg
     3901 tagtgaaaga cggtctcgga gccgacctcg cccgtcgcgg aattcgagac gggcgcgaag
     3961 aagcgttcgt cgtagtcgaa ggcgcccaac cgtcggctcc tcttaagggt tcgatgaaac
     4021 gggcgggtat ggtcgcacgg tcttgacgcg gttcgcaaac ttctcggcga ccttgttgtg
     4081 aatcttcttg ccgaagagga cggagcgcgt cttgccgctg gtgaggacgg ccaacgtgtg
     4141 cgggtcgagg acggtgaagg tgaagcggcg cgtgagcgac ttgcgctcga tctcgatgac
     4201 gaggtctgcg tccgcgaggt tgcgcgtcac cgtcatgccc cacgcgcgga actccttgcg
     4261 cttgcggagc gagtcctcga cctccgccgc cttgacgaag accgtgttcg agcggacgta
     4321 gacgctgcgc gccgtgcgca gcaagcccgc cgggtcgctc gcgcgggtct gcgtcgcgtt
     4381 gtcggcatcg tcgtcgtcat cgtcgttgtc gtcggcactg ccgtcgtctt tggctgccgc
     4441 ggcctgctgc ggtgtctgct gccgctgcgt cagttgcgga ggcagcggcg ggagaacctg
     4501 cgcggcggct tgcgaggcgc cagccgcgac ggagacggcg gcgagcagaa ggaggatgag
     4561 aggtcgcgtg acgaaaatgt tcaagggggg cgtcggtcgg gccgaagggt tgttcggaaa
     4621 agcgtgcgtc gaaagtatag ccgtcgaagc gtgtgtcgaa gaggaagtcg aagaagtgtg
     4681 tgccatgttc gttgtctccg gtaaggtgtc tcgactgcgg cgggcaaccg cacggcggag
     4741 cgcggcggtg gtcgagcgca atcgcccggc agtttaaagg cgcggggccg cgcggcgtgg
     4801 cgcgcgctgt gacactttct tgattgtcac gttggcgcga agcttttgac ggcgggcgtc
     4861 ggcggggctt accctctcgg tcagttgacg cacacgcaca agagactatc cgaatcgggg
     4921 ggacgcagaa catgtcgagg ctgactatct ggcggctgtt gcttattacc ttcctctgcg
     4981 cgcagctcgc ctgcggcggg ctgctgtcgg gcgacagcgg cagcgacgtg gacgacgcgg
     5041 gcggcgcgcg gcccgcgccg cacttcgagc cgggcttcaa cctcttcagc cccgagcagg
     5101 acatcgagtt gggccgcatg tccgcgcgcg agatcgcgca gcaggtgccc gtgatgacgg
     5161 acgcgcgcac gacgcagtac gtccagcagc tcggcgcgaa gctcgccgcg cacgcgcccg
     5221 gcttcaagtt cccctaccag ttcgccgtcg tcggcacgcg cgagatcaac gccttcgcac
     5281 tgccgggcgg ccccatcttc gtcaacgccg gcaccatcgc cgccgcgaag aacgagggcg
     5341 agctggccgg ggtcatagcg cacgagatta cgcacatcgc cttgcgccac ggcacgaacc
     5401 aggcgtcgaa ggcttacatc gcgcagaagg gcctcggcat cctcagcgag atcgtgggcg
     5461 gcgacggcgg cgacctcggc tccatcgtca gcgccatcgg cggcgcgggc gccaacatga
     5521 tcttcctcaa gttcggtcgc accgccgaga cgcaggccga cctcgaaggc gcgcgcctgc
     5581 tggccgccgc cggctacgac ccgcgcgaca tggcaacctt cttcgagaag cttcagtcgc
     5641 agagcggcgg gcagcgcgtc cccgaaatgc tcagcgacca cccggacccc ggcaaccgca
     5701 tcgccgcgat caaccaagtg atgccttcgc tgcgcgtgag ccagaagccg gtgcgcgact
     5761 cgaacgagtt ccagcaggtg aaggcgcggc tgacgggcgg cgccgtcaag tcggaggccg
     5821 agccgcagcg cgtcggcccg cgcgacccga ccgacatcga gccgggagcg cggccaccgg
     5881 cgccttcatc cgagttccgg gagtttcaat cgaaggacgg ctcgttcgcg cttcagtacc
     5941 cggcgaactg ggacgccatc ggcgccccgg agggcgacgc gaatctgatc ttcgcgccca
     6001 aaggggctta cgggaagatg ggcgacagcc tctacgtctc gcacggcatc ttcatcggcg
     6061 tcgtcgcgcc gcaggccggc gacctcgccg ccgccaacga agccttcgtc cgccagcagc
     6121 tcgccgccaa cccggacttc cgcgtgcagc gcaaccccga gcgcatcgag tcgggcgggc
     6181 gccagtggct ccgcacgctc gtcgccgggc cttcggcggt cacgggcgtc atcgagatcg
     6241 acgtcatcta cacgtcggcg acctcggacg ggcgcctctt ctacctcatc acgatggcgc
     6301 ccgaagacga gttccagaac taccagcccg ccttcgagcg gatcatcggc agtctccggc
     6361 tgtcgcgtta gagggcggca ggtgcttgag gtgtaaagtg cgttgcagtt gaacaagctg
     6421 agggaacgtt aagaggcggt ct
//
LOCUS       DBNGOH010000641         3458 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1560835, whole genome shotgun sequence.
ACCESSION   DBNGOH010000641 DBNGOH010000000
VERSION     DBNGOH010000641.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3458)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3458)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3458
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1560835"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3381
                     /locus_tag="AC159P_12580"
     CDS             <1..3381
                     /locus_tag="AC159P_12580"
                     /inference="COORDINATES: protein motif:HMM:NF024907.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HIR3696328.1"
                     /translation="RINLAIALLNVPDAVGALKEARAAAIVAPDAPQPHYVLGLLAKS
                     ENRADDALASFQRVLKIDPRDVGANVQLGQLYAQQRKYTEAVAAFRTALAEEPYNGTA
                     LYNLGTALLRSGQREEGQRLVQQFQALRQSGAATIIGQNYLEQGRYAEAVASTGAEAD
                     LVDKSTPEVSFVEATTATLPTSATSAVAERELPRASAGGPVLFDADGDGDLDLSEATG
                     AALRLYKNDAGKFVDVTAQAGALSKDAGGAVLVAGDYDNDERPDLLSIREGALALYRN
                     EGGLRFADATATARLPPYPYVSYSGAFVDYDHDGDLDIFVAGRADRSKASTPGADPRG
                     VSFLTGYAPAPHLLLRNNGDGTFADQTAAAKLSGVREGAQAVAVVPTDYDNRRDVDLL
                     VVHSDAPPALYRNLRDGTFRDVAAEVGLDFKARACSAAAAGDMNKDGFTDFFFACGPG
                     LFALSDGKGRFNTRPAPPGSAGAAQFLDYDNDGLLDLLTVNSDATRAHVRVLRNTGDG
                     WADVSERAARNLDARPHGFAAGDVDGDGDTDLFVHAAPGGLKFLRNEGGNRNASVRVR
                     LAGKVSNRGGVGAKIEARAGSLWQKSETYSANPAPAPSDVLFGLGKRPSFDAVRVLWP
                     AGIVQAETGVAPAAASPSALNMTVTELDRKPSSCPYLYTWNGERFEFITDFMGGGELG
                     YWAAPGVRAAPDPDEYVRIRADQLKPRDGRYELRVTNELEEALFIDRLQLVAVAHPAA
                     VEVHPNEGLGSPTSGPLKLYATRDARPPRAATDEHGHDVLHRITRLDRAYPDDFELHT
                     LRGYADEHTLTLDLAPDHGSDARVQTASPSSTVTRPSVLSPQSSVLLLLTGWTDYAFS
                     SDNVAASQSGRTLRAPALQVRDAAGNWRTVIDNIGIPVGRPQTVVVDLEGKFLSASRE
                     VRLVTNMRIYWDRILVAESGAGSSSLAESPLRVERLDASVADLGWRGFSAETTPDGRE
                     PYSYDYRKVSSASPWKALPGRYTRLGDVRELLASIDDIFVVSRPGDELALSFDARRLA
                     PLPQGWTRTFLLYADGFSKEMDLNSSSPDQLAPLPFHGMKSYPYAAPEAYPSTPAHRA
                     YVESYNTRLVNAPVTQLRGPGVGGRGPGKAISSLTPGP"
BASE COUNT          555 a         1391 c         1034 g          478 t
ORIGIN      
        1 cgcatcaacc tcgccatcgc gctcctcaac gtgccggacg ccgtgggcgc cttgaaggag
       61 gcgcgcgccg ccgcaatcgt cgcgcccgac gcgccgcagc cgcactacgt cctcggtctg
      121 cttgccaagt ccgagaaccg cgccgacgac gcgctcgcct ctttccagcg cgtgctgaaa
      181 atagacccgc gcgacgtggg cgccaacgtg cagctcggcc agctctacgc gcagcagcgc
      241 aagtacacgg aggccgtcgc cgccttccgc accgcgctcg ccgaagagcc ttacaacggc
      301 accgcgctct acaacctcgg caccgccttg ctgcgctcgg gtcagcgcga agaggggcag
      361 cgcctcgtcc agcagttcca ggcgctccgt cagagcggcg ccgcgaccat catcgggcag
      421 aactacctcg aacagggccg ctacgccgaa gccgtcgcct cgaccggcgc cgaagccgac
      481 ctcgttgaca agagcacgcc cgaagtctct ttcgtcgaag caacgaccgc gacgctgccg
      541 acttctgcga cgagcgccgt cgccgagagg gaactcccgc gggcgtccgc gggcggcccc
      601 gtgctcttcg acgcggacgg cgacggcgac ctcgacctct ccgaagcgac cggcgccgcg
      661 cttcggctct acaagaatga cgcgggcaag ttcgtggatg tgacggcgca ggcgggcgcg
      721 ctctcgaaag acgcgggcgg cgccgtcctc gtcgcgggcg actacgacaa cgacgagcgc
      781 cccgacctct tgtcgatacg cgaaggcgcg ctcgcgctct accgtaacga aggcggcctg
      841 cgcttcgccg acgcgaccgc gaccgcgcgc ctccctcctt acccttacgt ctcgtactcc
      901 ggcgccttcg tcgattacga ccacgacggc gacctcgaca tcttcgtggc tggccgcgcc
      961 gaccgctcga aggcgagcac gcccggcgcc gacccgcgcg gcgtcagctt cctcacgggc
     1021 tacgcgcccg cgccgcacct gctcctgcgc aacaacggcg acggcacgtt cgccgaccag
     1081 accgccgcag ccaaactctc cggcgtgcgc gagggcgcgc aggccgtcgc cgtcgtcccg
     1141 accgactacg acaaccggcg cgacgttgat ctgctcgtcg tccactccga cgcgccgccc
     1201 gcgctctacc gcaacctgcg cgacggcacc ttccgcgacg tggccgccga ggtcggactc
     1261 gacttcaagg cccgcgcgtg cagcgccgcc gccgcgggcg acatgaacaa ggacggcttc
     1321 accgacttct tcttcgcgtg cggccccggc ctcttcgcgc tcagcgacgg caagggccgc
     1381 ttcaacaccc gccccgcgcc gcccgggagc gcaggggccg cgcagtttct cgactacgac
     1441 aacgacggcc tgctcgatct cttgacggtc aacagcgacg cgacgcgcgc ccacgtccgc
     1501 gtcctgcgca acacgggcga cggctgggcg gacgtgagcg agcgcgcagc ccgcaacctc
     1561 gacgcacggc cccacggctt cgccgcgggc gacgttgacg gcgacggcga cacagatctc
     1621 ttcgtgcacg ccgcgcccgg cggcttgaag tttctgcgca acgagggcgg caacaggaac
     1681 gcctcggtgc gcgtgcgcct cgccggcaag gtgagcaacc gcggcggcgt cggcgcgaaa
     1741 atcgaggcgc gcgccgggag cctctggcag aagtcggaga cctactccgc taacccagcg
     1801 cccgcgccgt cggacgtgct cttcgggttg ggcaagcgcc cgagcttcga cgccgtgcgc
     1861 gtgctctggc ccgccggcat cgtgcaggcc gagacgggcg tcgcccccgc cgccgcgtcg
     1921 ccgagcgcct tgaacatgac ggtgacggaa ctcgaccgca agccttcttc ctgcccctac
     1981 ctctacacct ggaatggcga gcgcttcgag ttcatcacgg acttcatggg cggcggcgaa
     2041 ctcggctact gggccgcgcc cggcgtccgc gccgcgcccg accccgacga gtacgtccgc
     2101 atccgcgccg accagctcaa gccgcgcgac gggcgctacg agctgcgcgt caccaacgaa
     2161 ctcgaagagg cgctcttcat cgaccgcctc caactcgtcg ccgtcgcaca ccccgccgcc
     2221 gtcgaagtcc accccaacga aggactcggc agcccgacct ccggccccct caagctctac
     2281 gccacgcgcg acgcgcgccc cccgcgcgcc gccacggacg aacacggcca cgacgttctc
     2341 caccggatca cacgactcga ccgcgcctac cccgacgact tcgaactcca cacgctgcgc
     2401 ggctacgccg acgagcacac gctcaccctc gacctcgccc ccgaccacgg aagcgacgca
     2461 cgagtccaaa cagcgtcgcc ttcatcaacc gtcacccggc cctcagtcct cagtcctcag
     2521 tcctcagtcc ttcttctcct gaccggctgg acggactacg ccttttcgag cgacaacgtc
     2581 gccgcctcac agtcgggccg caccttgcgc gcgcccgcgc tacaggtgcg cgacgccgcg
     2641 gggaactggc gcaccgtcat cgacaacatc ggcatccccg tcggcaggcc gcagaccgtc
     2701 gtcgtcgatc tcgaaggcaa gttcctctcc gccagccgcg aggtgcgact cgtcaccaac
     2761 atgcgcatct actgggatcg gattctcgtc gccgagtcgg gcgccggttc gtcttcactc
     2821 gccgagtcgc ctttacgcgt cgaacgactc gacgcctccg tcgcagacct cggctggcgc
     2881 ggcttctccg ccgagaccac gcccgacggg cgcgagcctt actcctacga ctaccggaaa
     2941 gtctcctccg cctcgccctg gaaagccctg cccggtcgct acacacgctt gggcgacgtg
     3001 cgcgagttgc tcgcgtcgat cgacgacatc ttcgtcgtct cgcgccccgg cgacgaactc
     3061 gcgctctcct tcgacgcgcg ccggctcgcg ccgctccccc aaggctggac gcgcaccttc
     3121 ctcctctacg ccgacggctt cagcaaggag atggatttaa actcttcaag ccccgaccag
     3181 ctcgcgccgc tccccttcca cggcatgaag agctacccct acgccgcgcc cgaagcctac
     3241 ccctcgacgc ccgcgcaccg cgcctacgtc gagagctaca acacgcgcct cgtcaacgcg
     3301 ccggtaacac aactacgggg gccgggtgtc gggggccgtg ggccggggaa agcaatctct
     3361 tccctgaccc ccggcccctg acccccggcc cccttgcgag gttgtatgga acacctgaga
     3421 ttctccgccg ccgcctgcca gacagacatg ccgaaccc
//
LOCUS       DBNGOH010000642         2839 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4126224, whole genome shotgun sequence.
ACCESSION   DBNGOH010000642 DBNGOH010000000
VERSION     DBNGOH010000642.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2839)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2839)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2839
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4126224"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1537)
                     /locus_tag="AC159P_12585"
     CDS             complement(<1..1537)
                     /locus_tag="AC159P_12585"
                     /inference="COORDINATES: protein motif:HMM:NF024793.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HIR3696329.1"
                     /translation="MLGTTEISPPPTAARPRLVERMQQNLAHAVTLVTAPAGSGKTTL
                     VADFVRTHARKFVWYQLDHTDADPLVFLGYVTHGIKQVVPGFGAATASYMQEAAKELA
                     QRPERAVDVLLNEVLETVEQQFVLVLDDYHHLGAETPVHRVVDRLLAYLPDLIHVVIV
                     SRDVPPLALSRLRTQASLSVIDRSELLFTDDETRELFRNVFDLELTPEQLAEYRERTH
                     GWITALQLVRQVAQRQAIARGGASPASSSDLVEILRQSERDIFDYFAEEVFADEQEDV
                     RRFLLRVSLLERVEPETCSHLYPEAHNAAMLQTLARRNVFISLAADARGEEYRLHPLF
                     RSFLQRRFRVEAGRQRVAAEHARIAAHLLAREQWEQAVRHLLAAEDFDRAAATIAEHG
                     GAWISAGALASLAAFADALPRPALEAHPRALAHRAEVARLREEFDAAQSLFRRAAALL
                     HEQEDEEGEAEALHSLATLARRRGDCETAFNDLDRALELASEHSVVRTKCANTRGLCF
                     VALG"
     gene            complement(1743..2552)
                     /locus_tag="AC159P_12590"
     CDS             complement(1743..2552)
                     /locus_tag="AC159P_12590"
                     /inference="COORDINATES: protein motif:HMM:NF024589.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR3696330.1"
                     /translation="MARFLPDKMAAPSELPRVSRWRLLGLLKESLEGCNSTIIQGRAG
                     TGKTMLAADFARRCGRRAAWFKVDAPDAELDVFFRYLCASIAAERPGFGRGMLERVRA
                     SLGAEDVPLLVEYFVYELLESGEPLLVVIDDLHLVYDAGWVVPFFRRLLPLLPPEVHV
                     TLIGRSLPPTPLWRMRSKQTLCVLDEPTLAFTPEEARQLFASYGLDEARAESVLRQTR
                     GRAATFDAATRSLGVAEEAARLVAARDGNQDRGARALRLVKGFPKSSMGLV"
BASE COUNT          464 a          948 c          994 g          433 t
ORIGIN      
        1 gacccagagc gacgaagcag aggccgcgcg tgttggcgca cttcgtgcgg acgaccgagt
       61 gctcggacgc gagttcgagg gcgcggtcga ggtcgttgaa ggccgtctcg cagtcgccgc
      121 ggcggcgcgc gagcgtggcg agcgagtgca gcgcctcggc ctcgccctcc tcgtcctcct
      181 gctcgtgcag gagcgcggcg gcgcggcgga agagcgactg ggcggcgtcg aactcctcgc
      241 gcaggcgcgc gacctccgcg cggtgcgcga gcgcgcgcgg gtgcgcttcg agcgcggggc
      301 gcggcagcgc gtcggcgaag gcggcgagcg acgcgagggc gcccgccgaa atccacgcgc
      361 cgccgtgctc ggcgatggtc gcggcggcgc ggtcgaagtc ttccgccgcg agcaggtggc
      421 gcacggcctg ctcccactgc tcgcgcgcca gcaggtgcgc ggcgatgcgc gcgtgctcgg
      481 cggcgacccg ttggcgcccg gcctcgacgc ggaagcggcg ctggaggaag ctgcggaaga
      541 gcgggtggag gcggtactct tcgccgcgcg cgtccgccgc gagcgagatg aagacgttgc
      601 gccgcgcgag cgtctgaagc atcgccgcgt tgtgcgcctc ggggtagagg tggctgcacg
      661 tctccggctc gacgcgttcg agcagagaga cgcgcaggag gaagcggcgc acgtcctcct
      721 gctcgtcggc gaagacctct tcggcgaagt agtcgaagat gtcgcgctcg gactgtcgca
      781 gaatttcgac gaggtcggac gaagacgcgg gactcgcgcc gccgcgcgcg atggcctgtc
      841 gctgcgcgac ctgcctgacg agttgcagcg ccgtgatcca gccgtgcgtc cgctcgcggt
      901 actcggcgag ttgttccggc gtcagctcaa ggtcgaagac gttgcggaaa agctcccgcg
      961 tctcgtcgtc cgtgaagagc agctccgagc ggtcgatgac cgagagcgac gcctgcgtcc
     1021 gcaaccgcga gagcgcgagc ggcggcacgt cgcgcgagac gatgacgacg tggatcaagt
     1081 ccggcaggta ggcgagcagc cgatccacga cgcggtgcac gggcgtctcc gcgccgaggt
     1141 gatgataatc gtcgaggacg aggacgaact gctgctcgac cgtctccagc acttcgttca
     1201 acaagacatc gaccgcgcgc tcgggccgct gcgccaactc cttcgcggcc tcctgcatgt
     1261 aggaggcggt cgccgcgccg aagccgggca ccacctgctt gatgccgtgc gtgacgtagc
     1321 cgaggaagac gagcgggtcg gcgtccgtgt ggtcgagctg ataccagacg aacttgcgcg
     1381 cgtgggtgcg gacgaagtcg gcgacgagcg tggtcttgcc cgagccagcc ggggcggtga
     1441 cgagcgtgac ggcgtgcgcg aggttctgct gcatccgctc gacgaggcgc gggcgcgcgg
     1501 cggtcggcgg cggacttatc tctgttgttc cgagcacagc cgggctgcct tttacattag
     1561 cgtaaacgga cgacgcttgc gaggataaaa agaggaaatg atgaatgcgg aatgatgaat
     1621 gatgaatgag cgcacaggat gaatgatgag aagcagcaca cctctcatca ttcatcccgt
     1681 cattcatcat tcaagtcttc ttcttattca tcattcatca ttccgcactc atcatttcct
     1741 cttcagacca gccccatcga ggacttgggg aagcctttga cgaggcggag ggcgcgcgcg
     1801 cccctgtctt ggttgccgtc gcgcgcggcg acgaggcgcg ccgcctcttc ggcgacgccg
     1861 agcgagcgcg tcgccgcgtc gaacgtcgcg gcccgcccgc gcgtctgccg gaggacgctt
     1921 tcggcgcgcg cctcgtcgag gccgtagctg gcgaagagct gccgcgcctc ctccggcgtg
     1981 aaggcgagcg tcggctcgtc gagcacgcac agcgtctgct tcgagcgcat gcgccacagc
     2041 ggcgtcggcg gcaggctccg cccgatcagc gtcacgtgca cctcgggcgg cagcagcggc
     2101 agcaggcggc ggaagaacgg cacgacccac cccgcgtcgt agacgaggtg caggtcgtcg
     2161 atgacgacga gcagcggctc gcccgactcc aacagctcgt agacgaagta ctcgaccagc
     2221 agcggcacgt cctccgcccc gagtgacgcg cgcacgcgct cgagcatccc gcgcccgaag
     2281 ccgggccgct ccgccgcgat gctcgcgcac aggtagcgga agaagacgtc cagctccgcg
     2341 tccggcgcgt ccaccttgaa ccacgccgcg cgccgcccgc accgccgcgc gaagtccgcg
     2401 gcgagcatcg tcttgcccgt cccggcccgc ccctgaatga tcgtcgagtt gcagccttcg
     2461 aggctctcct tcaacagccc cagcagccgc caacgcgaca cgcgcggcag ctccgacggc
     2521 gccgccatct tgtccggaag aaatcgagcc atccgcgtca ctcttcgaat ctcttcggcg
     2581 gtgaggcttc ccgcgcgctc ctgaaaattc tggccggggc atcaggccac ttctcttcac
     2641 gaccgaacgc cgccacctcc gcggcgtccc tcacacacgc gaatttacgt ggagcacctt
     2701 gaggaattct tgagcggcga ccgaaggtca gggcgggacg gctaaatcgt tgattttggc
     2761 gggaaattcg accgggactg aaagcgggac gcgcgttcta cgcttcgctc aagagcggtt
     2821 tgcccttgag tttaacgac
//
LOCUS       DBNGOH010000643         3093 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_277422, whole genome shotgun sequence.
ACCESSION   DBNGOH010000643 DBNGOH010000000
VERSION     DBNGOH010000643.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3093)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3093)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3093
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_277422"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            129..1067
                     /locus_tag="AC159P_12595"
     CDS             129..1067
                     /locus_tag="AC159P_12595"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011141892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR3696331.1"
                     /translation="MGSNGSDVIPQRPFGRTGVRVSALGLGGYHLGMVKSEREAVRIV
                     QEAIAGGITFLDNAWEYHDGRSEELMGKAIGDRREEVFLMSKVCTHGRDRRTAMRQLE
                     QSLLRLKTDHLDLWQIHEVVYENDPDLHFAPGGAVEALEQARKEGKVRFVGFTGHKHP
                     SIHLKMLAHDFPFDACQLPLNVFDATFRSFEQLVLPELNRRGIAPLGMKSMCGEGAPV
                     RKRAVSAADALRYAMSLPVSTTFSGIDSLKVLRQNLRLARGFEPLTADEMQALRERCA
                     PDAADGRFELYKVSMQFEGPPGRRQHKFPPLEEVAA"
     gene            1103..2059
                     /locus_tag="AC159P_12600"
     CDS             1103..2059
                     /locus_tag="AC159P_12600"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HIR3696332.1"
                     /translation="MPISLTIETATVSGAVADLTGAPARSVERLPASAVNRVYKVSTE
                     AGGAFVVKVFRYADWPEEGKLSCVERKLTERGVPHPRLIHVSRDADLFPHGFSVTEHV
                     EGHDCAAAIAGGDLSIADYCRGIGSLLRKVHAVGFDRFGHLANCEGTDEDFVEAKLAY
                     EVNDRLGEIDAAALPPTDLAPRIEDKVRRLLAPFARRFAPVLVHSDAAPRNAILTDAG
                     EFVLADWDEALASIALEDYAKLTYLLFHAHDAPRLDAEARGVVRGSFFEGYGETGFDA
                     DETRAIEDALHTIQAADYLSYQFKTGNAAAFETTKELLLSLL"
     gene            2124..2429
                     /locus_tag="AC159P_12605"
     CDS             2124..2429
                     /locus_tag="AC159P_12605"
                     /inference="COORDINATES: protein motif:HMM:NF019491.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HIR3696333.1"
                     /translation="MLTHIVIWKYRADVSAERRAEHIARLRALVSVIPEIESFAVGAD
                     VLRLPRSYDTGLVAVFRDRAGLDAYTVHPEHVKVADLGREISEHVASVDFDDEPDAG"
     gene            2547..>3093
                     /locus_tag="AC159P_12610"
     CDS             2547..>3093
                     /locus_tag="AC159P_12610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HIR3696334.1"
                     /translation="MKRWKKIALALLLLLLLSQAPFVYRRYRLGRLSAAIAGLNAQRA
                     APPEDGYADHKGVFHVHSFLGGHSTGTFEEIVHAARRNDLHFVVMTEHPTAHVNTSEA
                     TLKGVHDGVLFINGSEIATKSGERLFIIPGVASDAAAATAPELVAKSKETGRLVFLAY
                     PEQVRDLAAPGAYDGIEVYNLY"
BASE COUNT          565 a         1104 c          938 g          486 t
ORIGIN      
        1 ccgatatctt cgacactcat gtcatacttt gcgaaacttt ctcggccatc aaaccggttc
       61 cgattcgcgc cgcgccgcac tcggaacgta tgcgcgcggc aaggagattg aaaggatatc
      121 aagcggtcat gggcagcaac ggttcggacg tgattccgca aaggccattc gggcgcacgg
      181 gcgtgcgcgt gtcggcgctc gggctgggcg gctaccacct cggcatggtc aagagcgagc
      241 gcgaggccgt ccgcatcgtg caggaggcaa tcgcgggcgg catcaccttc ctcgacaacg
      301 cgtgggagta ccacgacggg cgcagcgaag agttgatggg caaggccatc ggcgaccgcc
      361 gcgaagaggt cttcctgatg tcgaaggtct gcacgcacgg gcgcgaccgc cgcaccgcga
      421 tgcgccagct cgaacaatcc ttgctccgcc tgaagacgga tcacctcgac ctctggcaga
      481 ttcacgaggt cgtctacgag aacgaccccg acctgcactt cgcgcccggc ggcgccgtcg
      541 aagcgctcga acaggcccga aaggaaggca aggtgcgctt cgtcggcttc acgggccaca
      601 agcacccttc gatccacctc aagatgctcg cgcacgactt ccccttcgac gcgtgccagc
      661 tccccctgaa cgtcttcgac gccaccttcc gcagcttcga acagctcgtg ctccccgaac
      721 tgaacaggcg cggcatcgcg ccgctcggca tgaagagcat gtgcggcgaa ggcgcgcccg
      781 tccgcaagcg cgccgtctcc gccgccgacg cgctgcgcta cgcgatgagc ctgcccgtct
      841 cgacgacctt cagcggcatc gactcgctga aagtcctgcg ccagaacctc cgcctcgcgc
      901 gcggcttcga gccgctcacc gccgacgaga tgcaagcctt gcgcgagcgc tgcgcgccgg
      961 acgccgccga cgggcgcttc gaactctaca aggtgtcgat gcagttcgaa ggcccgcccg
     1021 gtagacgaca gcacaagttc ccgccgctcg aagaggtcgc ggcgtgacgc cgaggcccgc
     1081 gtcgaagtct cggccctgaa ccatgcccat cagcctgacc atcgagacgg ccaccgtctc
     1141 cggcgccgtc gcggatctga ccggagcgcc tgcgcgttcc gtcgaaaggc tcccggcgag
     1201 cgcggtcaac cgcgtctaca aggtctcgac ggaggcgggc ggcgccttcg tcgtcaaggt
     1261 cttcaggtac gcggactggc ccgaggaagg caagctctct tgcgtcgagc gcaagctcac
     1321 cgagcgcggc gtcccgcacc cgcggctcat ccacgtgtcg agggacgccg acctcttccc
     1381 gcacggcttc agcgtgaccg agcacgtcga ggggcacgac tgcgccgcgg cgattgccgg
     1441 cggcgacctc tccatcgcag actattgccg cggcatcggc tctctgctca ggaaggttca
     1501 cgcggtcggg ttcgaccgct tcggccacct cgccaactgc gagggcacgg acgaggactt
     1561 cgtcgaggcg aagctggcat acgaggtcaa cgacaggctg ggtgaaatcg acgcggcggc
     1621 gcttccaccg accgacctcg cccctcgcat cgaagacaaa gtgcgtcgtc tgctcgcccc
     1681 gttcgcgcgc cgcttcgcgc ccgtgctcgt ccactccgac gccgcgccgc gcaacgcgat
     1741 tctgacggac gcaggcgagt tcgtgctcgc cgactgggac gaggcgctcg cgagcatcgc
     1801 gctcgaagac tacgcgaagc tgacttacct gctcttccac gcgcacgacg cgccccgcct
     1861 cgacgcggag gcacgaggcg tcgtcagagg ctccttcttc gaaggctacg gcgagacggg
     1921 tttcgacgcc gacgagacgc gggcaatcga ggacgcgctg cacacgatac aggcggcgga
     1981 ctatctctct taccaattca agaccggcaa cgccgccgcc ttcgagacga cgaaggaatt
     2041 gctgctcagc cttctctgag cggttcgaca cgaagcgact tcaacagttc cactcagccc
     2101 gaccgcggag gtcttcgcaa ctgatgctca cgcacatcgt catctggaaa taccgcgccg
     2161 acgtaagcgc cgagcggcgc gccgaacaca tcgcacggct gcgcgcgctc gtcagcgtca
     2221 tccccgagat cgagagcttc gccgtcggcg cggacgtgct ccgcctgccg cgctcttacg
     2281 acaccggcct cgtcgccgtc ttccgcgacc gcgccggact cgacgcctac accgtccacc
     2341 ccgaacacgt gaaggtcgca gacctcgggc gcgagatcag cgagcacgtc gcctccgtcg
     2401 atttcgacga cgaaccggac gccggttgat tttggatttc ggatttcgga ttgacgaatg
     2461 acgatggatg acagcgccgc tcccatcaat cgtcaaccct tcttctaatc cagaatccaa
     2521 aatccaatag gctttaatag acgcggatga aacgctggaa gaaaatcgcg ctcgcgctgc
     2581 tgctcctgct gctcctctcg caggcgccgt tcgtctacag gcgctaccgt ctgggccgcc
     2641 tgagcgccgc gattgccggg ctgaacgcgc agcgcgctgc gccgcccgaa gacggctacg
     2701 ccgaccacaa aggcgtcttc cacgtccact ccttcctcgg cggccacagc acaggcactt
     2761 tcgaagagat cgtccacgct gcgcgcagga acgacctcca cttcgtcgtc atgaccgagc
     2821 accctacggc ccacgtcaac acgtcggagg cgacgctgaa gggggttcac gacggcgtcc
     2881 tcttcatcaa cggcagcgag atcgccacga agagtggcga gcgtctcttc atcatccccg
     2941 gcgtcgcgtc ggacgccgcc gccgcgaccg cgccggaact cgtcgcgaag tcgaaagaga
     3001 cggggcggct cgtcttcctc gcctaccccg aacaggtgcg cgacctcgcc gcgcccggtg
     3061 cctacgacgg catagaggtc tacaacctct aca
//
LOCUS       DBNGOH010000644         2540 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1744866, whole genome shotgun sequence.
ACCESSION   DBNGOH010000644 DBNGOH010000000
VERSION     DBNGOH010000644.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2540)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2540)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2540
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1744866"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            322..1269
                     /locus_tag="AC159P_12615"
     CDS             322..1269
                     /locus_tag="AC159P_12615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HIR3696335.1"
                     /translation="MKTTARPVQITPDVLRRLALPRVDEGGDKEERGRVLFVGGSRQM
                     PGAVVLASLAALRAGAGKLRVATATSTAPLVAAQVFEARVYPLAETKAGDIAAAAARR
                     LGECAEGARAICVGPGMMDEKGAARLVRKLLSSKTRATLVLDANALAGLRGSRRLVRG
                     LERGAVLTPNGDEVGGLLGGEDPRRRAGAGKTSAEAEDKAAAAADAARRIADEYGAVA
                     VVKGRETFIAAPGGELYVNLAGNVGLATSGSGDVLAGVVAGLAARGADALAAAVWGVH
                     GHAHAGDRLAERVGRLGYLARELLDEIPRVLEDLEAEAK"
     gene            1300..1545
                     /locus_tag="AC159P_12620"
     CDS             1300..1545
                     /locus_tag="AC159P_12620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015155713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbtH family protein"
                     /protein_id="HIR3696336.1"
                     /translation="MDRGDREDDTIYKVVVNHEEQYSIWPADRENALGWNDVGKSGTK
                     AECLAYIEEVWTAQRPSGLREKRGSAAAREDDAAVEE"
     gene            1542..>2540
                     /locus_tag="AC159P_12625"
     CDS             1542..>2540
                     /locus_tag="AC159P_12625"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025059.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3696337.1"
                     /translation="MSGEVGVDRSKSSGSVVEFVGALEESLRGGTFVKLTLGKYRGGE
                     AGLKNVYVRAVNIKGADRLSFLTRYRTKDVVKNYEVTEGVRRIESLLGASFLSGHLFT
                     TARDARIEFNKKREARLSERPPTFREAPPREHDRGKQRLIELEGNVYLRALGVTNERG
                     EVRGGMGDKFRQINKFVEIVAGLFDSSPLKDRREVGVVDMGAGKGYLTFAVYDYFSRV
                     RGMKATVTGIEARRELVARGNAIAREAGFDGLRFETGLIQDYAPEGVDILIALHACDT
                     ATDDAIFKGVAARASIVITAPCCHKEVRPQIETPEALGGVLRHGILLEREAETVTDS"
BASE COUNT          455 a          783 c          954 g          348 t
ORIGIN      
        1 ccgacatgct cgcgctcgcg aaccgtctgc ttcccggccc cggcggcata ggcaagcgcc
       61 gcgcgaaggg caaggagagc gagtcggcgc tcgcgccctc gctcctgacg gcgctcagcg
      121 acagcgcggc ggtgcggaac aacgaagtgc catagaccgt cgaacaaact taaccgcgaa
      181 ggcactgagg attttggatt ttagatttcg gattttagat taaaggcgaa tagccctttg
      241 gcattggatg gcggcgccgc tcccgccagc cgtcaatgtt tctcctaaac caaaatccaa
      301 aatctaaaat ccaaaatgtc gatgaagacc acagcgcgcc ccgttcaaat cacgccggat
      361 gtcttgaggc gcttggcgct gccgcgcgtg gacgaggggg gcgacaagga ggagcgcggg
      421 cgcgtgctgt tcgtcggcgg ctcgcggcag atgccgggcg cggtcgtgct cgcgtcgctg
      481 gccgcgctca gggcgggcgc gggtaagctg cgcgtggcga ccgcgacgag caccgccccg
      541 ctcgtcgcgg cgcaggtctt cgaggcgcgc gtctaccctc tggccgagac gaaggcgggc
      601 gacatcgcgg cggcggcggc gcgtcggctc ggcgaatgcg cggagggcgc acgcgcgatc
      661 tgcgtcgggc cggggatgat ggacgagaag ggcgcggcgc ggctcgtcag aaaacttctg
      721 tcgtcgaaga cgcgcgcgac gctggtgctc gacgcgaacg cgctcgcggg cctccgcggc
      781 tcgcggcggc tcgtgcgcgg gcttgagcgc ggggcggtgc tgacgccgaa cggcgacgag
      841 gtgggcggac tgctcggcgg cgaagaccct cggcgccgcg ccggagcagg gaagacatcc
      901 gccgaagcgg aggataaggc agccgcagcg gcggacgccg cgcgccgcat cgcggacgag
      961 tacggggcgg tcgctgtcgt caaagggcgc gagactttta tcgcggcgcc gggcggcgaa
     1021 ctctacgtga acctcgcggg aaacgtgggg ctggcgacat ccggctcggg cgacgtgctg
     1081 gcgggcgtcg tcgcgggcct cgcggcgcgc ggcgcggacg cgctcgcggc ggcggtgtgg
     1141 ggcgtccacg ggcacgctca cgcgggcgac cggctggccg agcgcgtagg gcggctcggc
     1201 tatctcgcgc gcgaactcct cgacgaaatt ccgcgcgtgc tcgaagacct cgaagcggag
     1261 gcgaagtaaa accctcgaca cccacgaagg agaacgctga tggacagagg cgatagagaa
     1321 gacgacacga tctacaaagt ggtggtcaac cacgaggagc agtattcgat ctggcccgcc
     1381 gaccgcgaga acgcgctcgg ctggaacgac gtgggcaagt cggggacgaa ggccgagtgc
     1441 ctcgcttaca tcgaggaggt ctggactgcc cagcgcccgt ccggcctgcg agagaagagg
     1501 gggagtgcgg cggcgcggga ggatgatgca gccgtagaag aatgagcggc gaagtcggcg
     1561 tcgatagaag caagagcagc ggatcggtcg tcgagttcgt cggcgcgctg gaagagagtc
     1621 tgcgcggcgg caccttcgtc aagctgacgc tcggcaagta ccgcggcggg gaggccgggc
     1681 tgaagaacgt ctacgtccgc gcggtcaaca tcaagggcgc cgaccgcctc tctttcctca
     1741 cgcggtacag gacgaaggac gtggtgaaga actacgaggt gaccgagggc gtccggcgga
     1801 tcgagtcgct gctcggcgcg tcgttcctga gcggccacct gttcacgacc gcgagggacg
     1861 cgcggatcga gttcaacaag aagagggaag cgcgtctgtc cgagcgcccg ccgactttca
     1921 gggaggcgcc gccgcgcgag cacgaccgcg gcaagcagcg gctcatcgag cttgagggga
     1981 acgtctacct gcgcgcgctc ggcgtgacga acgagcgcgg cgaggtcagg ggcgggatgg
     2041 gcgacaagtt ccggcagata aataaattcg tcgagatcgt ggccggactc ttcgactcgt
     2101 cgccgctgaa ggacaggcgc gaggtcggcg tcgtggacat gggcgcgggc aaaggttatt
     2161 tgacgttcgc ggtctacgac tacttcagcc gcgtgcgcgg gatgaaggcg accgtgaccg
     2221 gcatcgaggc gcggcgggag ctggtcgcgc gtgggaacgc gatcgcgcgc gaggcgggct
     2281 tcgacggcct gcgcttcgag accggcctca tccaggacta cgcgcccgag ggcgtggaca
     2341 tcctcatcgc gctccacgcc tgcgacacgg ccaccgacga cgccatcttc aagggagtcg
     2401 ccgcgcgcgc gtcaatcgtc atcaccgcgc cgtgctgtca caaggaggtg aggccgcaga
     2461 tcgagacgcc cgaggcgctc ggcggcgtgc tgcgccacgg catcctgctc gaacgcgagg
     2521 ccgagaccgt caccgactct
//
LOCUS       DBNGOH010000645         3234 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3944882, whole genome shotgun sequence.
ACCESSION   DBNGOH010000645 DBNGOH010000000
VERSION     DBNGOH010000645.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3234)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3234)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3234
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3944882"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..556
                     /locus_tag="AC159P_12630"
     CDS             <1..556
                     /locus_tag="AC159P_12630"
                     /inference="COORDINATES: protein motif:HMM:NF024755.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HIR3696338.1"
                     /translation="AFPLLELRRAGLFNRREHLTFYHHRLLFPFVVEGRARYLQARTT
                     AAGVEPRWHNMRGAVPALYNVDALGRLASGEVVYLVEGFTDTLTLAAHGFDAVGLVGA
                     AGLKAEWLAPLGRFQVVAALDPDAAGRRAAARYEELFAARGLKLLTVNLPSDVNDFFR
                     QRPSAALEFQLMTERAVEMMNAEC"
     gene            658..1956
                     /locus_tag="AC159P_12635"
     CDS             658..1956
                     /locus_tag="AC159P_12635"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HIR3696339.1"
                     /translation="MADETTKTTPPAAGLSVTDKLRLLLDITKKISRSLNVEEVLVLV
                     MDTLDSLLPYDAAGIYIIRRDPHISEGGTHTLFFHAEAVRGYDLDELLELRLKAGEGL
                     IGHVAATGETIVVADVRQDPRYVNARRETRSEMVAPIFSNDEVIGVFDLESDQVNTYT
                     EDDKQVLQLLASQVAIIVEKAMLHDQVVEKKRLEAQLEVARHVQLSLLPPRDPVVEGF
                     DISAYNFSTEEVSGDYYDFVKLYEDQLGLVIADVSGKGIPASLLMAFLRASLRAAIHI
                     GYAPHVAMGKVNYLLWETIEPHQYVTAFYGMLDATNRTLAFVNAGHNPPLLVNPDGSV
                     RVIERGGLPLGMFRDTRYYEYYLPIEPGQILLLYTDGATEANSPDGEEYGRERLVAAV
                     RAAQDRTARDMIDFIYNDVFEWSGGRGAGDDVTFVVIKAQ"
     gene            complement(2132..2389)
                     /locus_tag="AC159P_12640"
     CDS             complement(2132..2389)
                     /locus_tag="AC159P_12640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696340.1"
                     /translation="MATYRDPVRVFAPLEVIERLLEIRADAGYTPTIFLRSADDLASY
                     FDAYGFDVYSFDGAEAEFRYGHYRAPLAPGGDHAHESMTAH"
     gene            2571..3176
                     /locus_tag="AC159P_12645"
     CDS             2571..3176
                     /locus_tag="AC159P_12645"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF017532.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HIR3696341.1"
                     /translation="MTTPDLLRRFGLFAVENFLDARECLDLCEKMRAGRAGKATVFKK
                     ADYVYDEEVRRTKTARVTGEARLFVASRLHAIMPDAGRHFGLNLTDTEEPQFLIYQPG
                     DFFRLHRDRGPDETTTAHTRRRAVSLVLMLNDPSEEPRAGAYGGGSLVLYGLGGDPRL
                     ARIGFPVRGAAGLLVAFDSELFHEVTPVAHGERFTVVSWFN"
BASE COUNT          561 a         1120 c         1005 g          548 t
ORIGIN      
        1 cgccttcccg ctcctagagc tgcgccgtgc cggactcttc aaccgcagag agcacctgac
       61 gttctatcac caccggctgc tcttcccgtt cgtcgtcgag ggccgcgccc gctacctaca
      121 ggcgcgcacg accgccgccg gggtcgagcc gcgctggcac aacatgcggg gcgcggtgcc
      181 cgcgctctac aacgtggacg cgctcgggcg gctggcgagc ggcgaggtcg tctacctcgt
      241 cgaaggcttc accgacacgc tgacgctcgc ggcgcacggc ttcgacgccg tggggctggt
      301 cggcgccgcg ggcctcaagg ccgaatggct cgcgccgctc gggcgcttcc aagtcgtcgc
      361 cgcgctcgac ccggatgccg ccggtcgccg cgccgccgcg cgctacgaag agctgttcgc
      421 cgcccgcggc ctgaaactcc tgaccgtcaa cctcccttcg gacgtgaacg acttcttccg
      481 ccaacgcccg tccgccgcgc ttgagttcca gttgatgacc gagcgggcgg tagaaatgat
      541 gaacgcggaa tgctgaatga tgaatgagag gacaagtcgg acgatgaaag gtaaagcgct
      601 tttcgttcat cgttcatcat tccgcattcc gcattcatca tttataatcc ccgccttatg
      661 gccgacgaga ccacgaagac gaccccgccc gccgcgggct tgagcgtgac ggacaagctg
      721 cggctgctct tggacatcac gaagaagatc agccgctctt tgaacgtcga ggaggtgctg
      781 gtgctggtga tggacacgct cgactcgctg ctgccttacg acgccgcggg catctacatc
      841 atccgccgcg acccgcacat cagcgagggc ggcacgcaca ccctcttctt ccacgccgag
      901 gccgtgcgcg gctacgacct cgacgagctg ttggagctgc ggctgaaggc gggcgagggc
      961 ctcatcggcc acgtcgccgc cacgggcgag acaatcgtcg tcgcggacgt gcgccaagac
     1021 ccgcgctacg tcaatgcgcg cagggagacg cgctccgaga tggtcgcgcc catcttctcg
     1081 aacgacgagg tgatcggcgt cttcgacctc gaatccgacc aggtgaacac ctacaccgag
     1141 gacgacaagc aggtcttgca gctcctcgcc tcgcaggtgg cgatcatcgt cgagaaggcg
     1201 atgctgcacg atcaggtggt cgagaagaag cggctcgaag cgcagctcga agtcgcgcgc
     1261 cacgtccagc tctcgctgct cccgccgcgc gacccggtcg tcgagggctt cgacatcagc
     1321 gcctacaact tctcgaccga ggaggtctcg ggcgactact acgacttcgt caagctctac
     1381 gaggatcagc tcggcctcgt catcgccgat gtgtccggca agggcatccc ggcctcgctg
     1441 ctgatggcct tcctgcgcgc ctctctgcgc gccgcgatcc acatcggcta cgcgccgcac
     1501 gtggcgatgg ggaaggtcaa ctacctcctg tgggagacca tcgagccgca ccagtacgtg
     1561 acggccttct acggaatgct cgacgcgacg aaccgcacgc tggccttcgt caacgccggc
     1621 cacaaccccc cgctcctcgt caaccccgac ggcagcgtgc gcgtcatcga gcgcggcggc
     1681 ctcccgctcg gcatgttccg cgacacgcgc tactacgaat actacctgcc gatcgagccg
     1741 ggccagattc tgctgctcta cacggacggc gcgaccgagg ccaacagccc cgacggcgaa
     1801 gagtacgggc gcgagcgcct cgtcgccgcc gtgcgcgccg cgcaagaccg caccgcgcgc
     1861 gacatgatcg acttcatcta caacgacgtg ttcgagtggt cgggcgggcg cggcgcgggc
     1921 gacgacgtga cgttcgtcgt catcaaggcg cagtaggcag taggcagcgg tcagtaggca
     1981 gtcaaaacca aaagagtgaa gaagcttgag aagtaaagtg ccaagcatcc tcgctcttca
     2041 agcgacaaag caagaggctt gggactttac ctcccaagcc tcttcttttt gtcttgactg
     2101 cctaccgcct actgcctact gcctactacg ctcagtgcgc cgtcatggac tcgtgggcgt
     2161 ggtcgccgcc gggggcgagc ggggcgcggt agtggccgta gcggaactcg gcctcggcgc
     2221 cgtcgaacga gtacacgtcg aagccgtagg cgtcgaagta ggaggccaaa tcgtccgccg
     2281 agcgcaggaa gatggtgggc gtgtagcccg cgtcggcgcg gatttccagc aggcgctcga
     2341 tcacctcgag cggggcgaaa actctcacag ggtctctgta ggttgccatc ttgcttctcg
     2401 cgcctccttt cccgtccgtc agcgttcgtc gccggaattg tgttccggtg ctattcagat
     2461 gtacgagaaa gaggtcgcgg ttggttgctt tcgtcgttta atttcggtta caagattcac
     2521 gagagcgttg ccggcctcgc tcgctctgac ccggcaccgc cgccccaccg atgacgaccc
     2581 ccgaccttct ccggcgcttc ggactgttcg cggtcgagaa cttcctcgac gcgcgcgagt
     2641 gtctggattt gtgcgagaag atgcgcgccg ggcgcgcggg gaaggcgacg gttttcaaaa
     2701 aggcggatta cgtatatgat gaagaagtgc gccgcaccaa aacggcgcgc gtcaccgggg
     2761 aggcgcgcct cttcgtcgcc tcccgcctgc acgcgatcat gcccgacgcc ggacgccact
     2821 tcggcctgaa cctgaccgac acagaggagc cgcagttttt gatctaccag ccgggggact
     2881 ttttccgcct ccaccgcgac cgcggccccg acgagacgac gaccgcccac acgcgccgcc
     2941 gcgccgtctc gctcgtcctg atgctcaacg acccgtcgga agagccgcgc gcgggcgcct
     3001 acggcggagg ctctctggtg ctctacggcc tcggcggcga cccgcgcctc gcgcgcatcg
     3061 gcttccccgt ccgcggcgcg gcgggcctgc tggtcgcctt cgactccgaa ctctttcacg
     3121 aagtcacgcc cgtcgcgcac ggcgagagat tcaccgtcgt gagctggttt aactaaagag
     3181 acccgaatca tggacacacc gcagacaccc gccgcgccgc cgaccccggc cccc
//
LOCUS       DBNGOH010000646         2665 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3760320, whole genome shotgun sequence.
ACCESSION   DBNGOH010000646 DBNGOH010000000
VERSION     DBNGOH010000646.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2665)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2665)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2665
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3760320"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..500
                     /locus_tag="AC159P_12650"
     CDS             <1..500
                     /locus_tag="AC159P_12650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696342.1"
                     /translation="ALVSQNVQVGVGPWPETLEANKVAYFNAFVARAAFTTAFPTTMT
                     PAQFVDKLNQNAEGALSQAERDTLVGELTSGAKSRAQVLRAVAEDETFAQSQFRRAFV
                     FMQYVGYLRRDPDAPDFRGVPDPNFEGFNFWLGKLNQFNGSYIDAEMVKAFITSIEYR
                     QRFGQ"
     gene            840..>2665
                     /locus_tag="AC159P_12655"
     CDS             840..>2665
                     /locus_tag="AC159P_12655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696343.1"
                     /translation="MSLSVLKKVVRPVLVLALLVQAAPLARAQETAAPRKVREQIRGV
                     RAEGARAARVNLAELQRQESLNPDDSYAVPRAIPAPKPAPEEPTDPEADPLPASSSIE
                     ADAPQPLGPSPAAAGFLGSEDGVNDGFRSIPPDTHGAVGPNHLVVTHNGRVRVQAKTG
                     QTLSTVGLKTFWSRLFPAKSLSEMDVFDPKMLYDPYGGRFIITSCADRNKPESAVLVG
                     VTQTSDPTGNWFLYSVDADPADLTWADFPSMGFNKNWVVVQMNMFAVGAGTSNAGKFF
                     VFDKAQLYAGPSSMGATVITVPTSGFTMVPAATYDPNLETLYVLQRWNGASGSLRVSA
                     INNVGGIPTLTTALSFPQVTNFTWQSTGAGQSDFAPQQGSVQKIQTNDDRMHSVVYRN
                     GSLWGAHTVFTPRGAPTRSTVQWWEVDPAVATGQFAAPRQRGLVDPTGTDYYAFPSIA
                     VNRHNDVLVGYTRFGPDKFAGANYSFRAGTDPSNSLRDDAVLKAGEAHYYKTFGGTEN
                     RWGDYSATTVDPSNDSDLWTLQEYAAARAGTGAADTDSRWGTWWGKVSVAAPADTVAF
                     AAATTSVSESAGRVDLVVRRAGAAAGAVSVDYATGNASATDL"
BASE COUNT          450 a          993 c          831 g          391 t
ORIGIN      
        1 aggcgctcgt ctcgcagaac gtgcaggtcg gcgtcggccc gtggccggag acgctcgaag
       61 ccaacaaggt cgcctacttc aacgccttcg tcgctcgcgc ggccttcacg acagccttcc
      121 cgacgacgat gacgcccgcg cagttcgtgg ataagctcaa ccaaaacgcg gaaggtgcgc
      181 tctcgcaggc cgagcgcgac acgctcgtgg gcgagttgac cagcggcgcg aagtcgcggg
      241 cgcaggtgct gcgcgccgtg gccgaggatg agacgttcgc gcagtcgcag ttccgcaggg
      301 cgttcgtctt catgcagtac gtcggctacc tgcgccgcga cccggacgcg cccgacttcc
      361 gcggcgtccc cgacccgaac ttcgaaggct tcaacttctg gctcgggaag ctcaaccagt
      421 tcaacggcag ctacatcgac gccgagatgg tgaaagcctt catcacctcg atcgagtacc
      481 gccagcgctt cggccagtaa agtaggggcg aaaaggggaa gtgggaaaag gggaaaaggt
      541 aaaaggggaa gaggggaaga ggtaaagaag cgggttgctt tcttcccttt ccccccttcc
      601 tctcttccga aatgtcttga cagtgccgcg ccgcgaaacc tggattgttt gttaaggcgc
      661 ccgcggcccg tcgagtgccc ctgacccttc ggcgtcccga cctcacccgt ggaatccgaa
      721 gctccccaac aaacggtttt cgccttcgag tccgccccgc cgccaagcgg cgggcggcga
      781 acaatgtccg gcgcgtgcgt gcggcccatg cgctctatag ccggtcggca acgtcgtaga
      841 tgtccttgag cgtgctgaaa aaagtcgtcc gccccgtcct cgtgctcgcg ctcctcgtgc
      901 aggccgcgcc gctcgcgcgc gcgcaggaga ccgccgcgcc gcgcaaagtc cgcgagcaga
      961 ttcgcggcgt gcgcgcagag ggcgcccgcg cggcgcgggt gaacctcgcc gagcttcagc
     1021 gacaggagtc gctcaacccc gacgactcgt acgccgtgcc gcgcgccatc cccgcgccga
     1081 agcccgcgcc cgaagagccg accgaccccg aagcggaccc gctccccgcg tcttcttcca
     1141 tcgaggccga cgcgccgcag ccgctcggcc cctcgcccgc cgccgcgggc ttcctcggct
     1201 ccgaagacgg cgtcaacgac ggcttccgca gcatcccgcc cgacacgcac ggcgcggtcg
     1261 gccccaatca tctcgtcgtc acgcacaacg gtcgcgtccg cgtccaggcc aagaccggcc
     1321 agaccctgag caccgtcggc ctcaagacct tctggtcgcg cctcttcccc gcgaagtcgc
     1381 tctccgagat ggacgtgttc gacccgaaga tgctctacga cccctacggc ggtcgtttca
     1441 tcatcacgtc ctgcgccgac cgcaacaagc ccgagtcggc ggtgctcgtc ggcgtcacgc
     1501 agacctcgga cccgaccggc aactggttcc tctacagcgt ggacgccgac ccggcagacc
     1561 tcacctgggc cgacttcccg agcatgggct tcaacaaaaa ctgggtcgtc gtccagatga
     1621 acatgttcgc cgtgggcgcc ggcacgtcga acgcgggcaa gttcttcgtc ttcgacaagg
     1681 cgcagctcta cgccgggccg tcgtcgatgg gcgcgaccgt catcaccgtt ccgacgagcg
     1741 gcttcacgat ggtgcccgcc gcgacctacg acccgaacct cgaaacgctc tacgtcctcc
     1801 agcgctggaa cggcgcgtcg gggagcctgc gcgtcagcgc catcaacaac gtcggcggca
     1861 tcccgacgct caccaccgcg ctctcgttcc cgcaggtcac gaacttcacg tggcagagca
     1921 ccggcgcggg gcaatccgac ttcgcgccgc agcaaggctc cgtgcagaag attcagacca
     1981 acgacgaccg catgcactcg gtcgtctacc gcaacggctc gctgtggggc gcgcacaccg
     2041 tcttcacgcc gcgcggcgcc ccgacgcgca gcaccgtcca gtggtgggag gtcgatcccg
     2101 ccgtcgccac cggccagttc gccgcgccgc gccagcgcgg cctcgtcgat cctacgggca
     2161 cggactacta cgccttcccc tccatcgccg tcaaccgcca caacgacgtg ctcgtcggct
     2221 acacgcgctt cggccccgac aagttcgccg gcgccaacta ctccttccgc gcgggcacag
     2281 acccctcgaa ctctctgcgc gacgacgccg tcctcaaggc cggcgaggcg cactactaca
     2341 agaccttcgg cggcaccgag aaccgctggg gcgactacag cgctacgacc gtcgatccct
     2401 cgaacgactc agacctctgg acgctacagg agtacgccgc ggcgcgcgcg ggcacgggcg
     2461 ccgccgacac cgacagccgt tggggcactt ggtggggcaa ggtctccgtc gcggccccgg
     2521 cggacacggt cgcgttcgcc gcggcgacaa cctccgtcag cgagagcgcg ggccgggttg
     2581 acctcgtggt gcggcgggcg ggcgccgccg cgggcgcggt ctcggtggac tacgcgacgg
     2641 gcaacgcctc ggcgaccgac ctcaa
//
LOCUS       DBNGOH010000647         2069 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1196444, whole genome shotgun sequence.
ACCESSION   DBNGOH010000647 DBNGOH010000000
VERSION     DBNGOH010000647.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2069)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1196444"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2069)
                     /locus_tag="AC159P_12660"
     CDS             complement(<1..>2069)
                     /locus_tag="AC159P_12660"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014026.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 3 protein"
                     /protein_id="HIR3696344.1"
                     /translation="QQQPPPPLSAEAERRVEALLKQLTLEEKVDLLGGTDGFFVRGVE
                     RLKLPRLKMADGPGGVRNFGPATAMPSGIALAASWNTQLAERVGTEIGRDARSKGVHF
                     LLGPGVNIYRAPMNGRNFEYFGEDPYLAARTAVGYIRGVQSQQVSATIKHYMGNNSEF
                     DRHNTDSVIDERTMREIYLPAFEAAVREARVGAVMDSYNLVNGEHASQSKHLLTDVAK
                     TEWGFDGVMMSDWFATYDGVAAANAGQDLEMPGPAHMNRKNLLPAIEQGKVSVATIDD
                     KVRRILRLAARFGWLDRDQTDLSVPRLNQQGRRVALEVAREGMVLLKNEGGLLPLDKR
                     KLKTIAVIGPTAYPAVPMGGGSGQVLPFNAVSILEGLGHALGAASSVTYHRGLPTHGE
                     LAEATEFVTAETNGEPGLKAEYFDNGDLKGAPVITRTERQVNFRRAPRGIVGELSVGF
                     PDRTVAERWMGYYVPKSAGEHDIFVESTGEDGGSFRLSLDDKVVLDNWTTHPAMVNYA
                     TLALDARPHKIVLEHRGRSQWLGGRLRLGVVRRGAAVDPQAKALAAKADVVIVAAGFD
                     PETESEAADRTFRLPPGQDELIRELAAANRNTVVVLTSGGGADRAGWLERVPALVAAW
                     YPGQEGGTAVAELLLGDVNPSGRLPVTFERRWEDNPAHASYYPEGNTKRVVYKEGVFV
                     GYRGYEK"
BASE COUNT          272 a          723 c          730 g          344 t
ORIGIN      
        1 tcttctcgta gccgcggtag ccgacgaaga cgccctcttt atagacgacg cgcttcgtgt
       61 tgccttccgg gtagtagctc gcgtgcgcgg ggttgtcctc ccagcggcgc tcgaacgtga
      121 cgggcaagcg gcccgaaggg ttcacgtcgc cgagcagcag ttccgcgacg gcggtgccgc
      181 cctcctgtcc cggataccac gccgcgacga gcgcgggcac gcgctccagc caccccgccc
      241 tgtccgcgcc gccgcccgac gtgaggacga cgacggtgtt cctgttcgcg gcggccagct
      301 cgcggatcag ctcgtcctgt ccgggcggca ggcggaacgt gcggtcggcg gcctcgctct
      361 cggtctcggg gtcgaagccc gcggcgacga tgaccacgtc ggccttcgcg gcgagagctt
      421 tggcctgcgg gtcaaccgcc gcgccgcgcc gcacgacgcc gagccgcaga cgccccccga
      481 gccactgcga gcgcccgcgg tgttcgagca cgatcttgtg cgggcgcgcg tcgagcgcga
      541 gcgtggcgta gttgaccatc gccgggtggg tcgtccagtt gtcgagcacc accttgtcgt
      601 cgagcgagag gcggaacgag ccgccgtcct cgcccgtgga ttcgacgaag atgtcgtgct
      661 cgcccgccga cttcgggacg taatagccca tccagcgctc ggccaccgtc ctgtcgggga
      721 agccgaccga cagctcgccg acgatgccgc gcggcgccct cctgaagttg acctgccgct
      781 cggtgcgcgt gatgaccggc gcccccttca ggtcgccgtt gtcgaaatac tcggccttga
      841 ggcccggctc gccgttcgtc tcggcggtca cgaactcggt cgcctcggcc agctcgccgt
      901 gcgtcggcag gccgcggtgg taggtgacgc tcgacgccgc tccgagcgcg tggcccaagc
      961 cttcgaggat gctgacggcg ttgaagggca ggacctgccc gctgccgccg cccatcggca
     1021 ccgccgggta ggccgtgggg ccgatgacgg cgatggtctt gagcttccgt ttatcgagcg
     1081 gcaacagccc gccctcgttc ttcaagagca ccatcccttc gcgcgcgact tcgagcgcga
     1141 cgcggcggcc ctgctggttg aggcgcggga cggagaggtc tgtctggtcg cggtcgagcc
     1201 agccgaagcg cgccgccaga cggaggatgc ggcgcacctt gtcgtcgatg gtcgcgaccg
     1261 agaccttgcc ctgctcgatg gccggcagca gattcttccg gttcatgtgc gccggccccg
     1321 gcatctccaa gtcctgcccg gcgttggccg ccgccacgcc gtcgtaggtg gcgaaccagt
     1381 cggacatcat cacgccgtcg aagccccact cggtcttcgc cacgtcggtc agcaggtgct
     1441 tgctctgcga cgcgtgctcg ccgttgacga ggttgtacga gtccatgacg gcgccgacgc
     1501 gcgcctcgcg cacggcggcc tcgaaggcgg gcaggtagat ttcgcgcatc gtgcgctcgt
     1561 cgatgacgga gtccgtgttg tggcggtcga actccgagtt gttgcccatg tagtgcttga
     1621 tggtggccga gacctgctgc gactgcacgc cgcggatgta gccgacggcg gtgcgcgcgg
     1681 ccaggtacgg gtcttcgccg aagtactcga agttgcgccc gttcatcggc gcgcggtaga
     1741 tgttgacgcc ggggcctaag aggaagtgga cgcccttcga gcgcgcgtcg cgcccgatct
     1801 cggtgccgac gcgctcggca agctgtgtgt tccaagaggc cgccagcgcg atgccgctcg
     1861 gcatggccgt ggccgggccg aagttgcgca cgccgccggg gccgtcggcc atcttcaggc
     1921 gcggcagctt gagccgctcg acgccgcgga cgaagaagcc gtcggtgccg ccgagcaggt
     1981 ccaccttctc ttcgagcgtt aactgcttga ggagcgcctc gacgcggcgc tcggcctccg
     2041 ccgacagcgg cggcggcggc tgttgctgc
//
LOCUS       DBNGOH010000648         2410 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3211306, whole genome shotgun sequence.
ACCESSION   DBNGOH010000648 DBNGOH010000000
VERSION     DBNGOH010000648.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2410)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2410)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2410
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3211306"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..285)
                     /locus_tag="AC159P_12665"
     CDS             complement(<1..285)
                     /locus_tag="AC159P_12665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010409016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HIR3696345.1"
                     /translation="MRRPKVITLFLFLLVLLGGGPSRARAQAAALAAQPAAGDESGFE
                     SIFDGKTLKGWEGDPKYWRVEGGALVGEVTPETLLKQNSFIIWRGGTTRDF"
     gene            complement(355..>2410)
                     /locus_tag="AC159P_12670"
     CDS             complement(355..>2410)
                     /locus_tag="AC159P_12670"
                     /inference="COORDINATES: protein motif:HMM:NF028442.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HIR3696346.1"
                     /translation="PRTVLFYFSSHTGQVVKRAAVGAEGYVLDHYQRTAIEKHLREAG
                     DKQLAAAGPGGVHSIFTDSLEVYDADWTADMFAEFKKRRGYDLKPLLPFLEYDAGERS
                     EALRRDFGRTLTELYEERFLVPMREWTTRNKVLFRIQNYGEPPASLSSSRHADLIDGE
                     GWNFRALTSSRWAASASHLFGKPVTTSETWTWLRSPAFRAAPLDVKGEADQHFLSGIN
                     QLIGHGWPYSPPQAGAPGWPFYASAVLSDKNPWWPVMPDLAAYLQRLSFLLRQGEPVA
                     DVALYAPSEDAWSTFKPGTPRYTNLFVKTVDWIGPNVVPAILDAGHSFDLIDDGTLKE
                     AQARRYKAVVLPGVRWMPEATRRWLAEYARGGGTVLAVRRKPEGDWPSLEVSSEAELS
                     RRLAAALPPDVTLQPAAPEIGFVHRRLKDADIYFMANTANVARKVSARFRDATPHVEA
                     WDPLTGKSERLESRGGEVALDFEPYGTRVVVFGKEAGSAPIAKARSVVASEELRTGWV
                     VSFGGADGGTTVELPHSWAEAPATRHFSGTAVYRRTVQLPAAFREPGTRVVLDFGEAK
                     PAEREPLPGGTLRGNSYAALVAPPVREAATVYVNGRRAGSLWAPPYRIDITELLRDGS
                     NEIRVDVYNTAINQLAEGGRLPDVRPLVERYGQRFRLQDLEGLRPLPSGIVAPVRLSA
                     EK"
BASE COUNT          359 a          802 c          871 g          378 t
ORIGIN      
        1 gaagtcgcgc gtcgtgccgc cgcgccagat gatgaaggag ttctgcttga gcagcgtctc
       61 cggcgtcacc tcgccgacga gcgcgccgcc ctcgacgcgc cagtatttcg ggtcgccctc
      121 ccagcccttg agcgtcttgc cgtcgaagat cgactcgaag ccggactcgt cgcccgccgc
      181 gggttgcgcc gccagagctg ccgcctgcgc gcgtgcgcgc gacggcccgc cgccgagtag
      241 gacgagcaag aaaaggaaca gtgtgatgac cttcggacgc cgcatccgtt ctctccgccc
      301 gcatggggtt acaggctcgt cggcatctcg cacgcgggcg cgcgactgcc gacctcactt
      361 ctccgccgac aggcgcaccg gcgccacgat cccggagggc aggggccgaa gtccttccaa
      421 gtcctgaagg cggaaccgct gcccgtagcg ttcgacgagc ggacgaacgt ccggcagccg
      481 accaccctcg gcgagctggt tgatggcggt gttataaacg tcaacccgga tctcgttcga
      541 gccgtcgcgc agcagttccg tgatgtctat ccggtaaggc ggcgcccaca acgatccggc
      601 gcgccggccg ttgacgtaga ccgtggcggc ctcgcggacg ggtggcgcga cgagcgcggc
      661 gtaggagttg ccgcgcaggg tgccgcccgg cagcggctcg cgctcggcgg gtttcgcctc
      721 gccgaaatcg aggacgacgc gcgtgcccgg ctcgcggaag gcggcaggca gttgaaccgt
      781 ccggcggtag accgccgtcc cggagaagtg gcgcgtggcc ggcgcttcgg cccacgagtg
      841 cggcaactcg accgtcgtgc cgccgtccgc cccgccgaag ctcacgaccc agcccgtgcg
      901 aagttcttcc gacgcgacga cggaacgggc tttcgcgatg ggcgcggagc cggcctcttt
      961 gccgaacacg acgacccgcg tgccgtaagg ctcgaagtcg agcgcgacct cgccgccgcg
     1021 cgactctaac cgttccgact tgcccgtgag cgggtcccag gcttcgacgt gcggcgtcgc
     1081 gtcgcggaag cgcgcgctga ccttgcgcgc cacgttggcg gtgttcgcca tgaagtagat
     1141 gtccgcatcc ttgagccggc ggtggacgaa gccgatctcg ggggcggcgg gctggagcgt
     1201 cacgtccggc ggcagcgccg cggccagccg gcgggagagt tcggcctcgc tcgacacttc
     1261 gagggagggc caatcgcctt cgggcttgcg ccgtacggcg agcaccgtcc cgccgccgcg
     1321 cgcgtactcg gcgagccagc gccgcgtcgc ctccggcatc cagcgcacac ccggcagtac
     1381 gacggccttg tagcgtcgcg cctgcgcctc tttgagcgtg ccgtcgtcga tcaggtcgaa
     1441 gctgtggccc gcgtcgagga tggccgggac gacgttcggc ccgatccaat caaccgtctt
     1501 cacgaacagg ttcgtgtagc gcggcgtgcc cggtttgaag gttgaccacg cgtcctccga
     1561 gggagcgtag agcgcgacat ccgccaccgg ctcgccctgg cgcaggagga agctcaggcg
     1621 ttggaggtag gccgcgaggt cgggcatcac gggccaccat gggttcttgt cgctcaagac
     1681 tgccgacgcg tagaagggcc agccgggcgc gcctgcctgc ggcggcgagt agggccagcc
     1741 gtggccgatg agttggttga tgccggagag gaagtgctgg tcggcttcgc ccttgacgtc
     1801 gagcggcgcg gcgcggaagg ccggcgagcg gagccacgtc cacgtctcgg aggtcgtgac
     1861 gggcttgccg aagaggtgcg aggccgacgc cgcccagcgt gaggaggtga gcgcgcggaa
     1921 gttccacccc tcgccgtcga tcaggtcggc gtggcgcgag cttgagagcg aggcgggcgg
     1981 ctcgccgtag ttctggatgc ggaagagaac tttgtttctc gtcgtccact cgcgcatggg
     2041 gacgaggaag cgttcctcgt agagttcggt gagcgtgcgc ccgaagtcgc gccgcagggc
     2101 ttcggagcgt tcgcccgcgt cgtattcgag gaacgggagc agcggcttga ggtcgtagcc
     2161 acggcgcttc ttgaactcgg cgaacatgtc cgccgtccag tccgcgtcgt agacctccaa
     2221 actgtcggtg aagatcgaat ggacgccccc cggcccggcg gcagcgagtt gtttgtcgcc
     2281 ggcctcgcgg aggtgttttt cgatggcggt ccgttggtag tggtcgagca cgtagccctc
     2341 ggccccgacg gcggcacgct tgaccacctg tcccgtgtga ctggagaagt agaaaagaac
     2401 cgtgcgcggg
//
LOCUS       DBNGOH010000649         3624 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2479162, whole genome shotgun sequence.
ACCESSION   DBNGOH010000649 DBNGOH010000000
VERSION     DBNGOH010000649.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3624)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3624)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3624
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2479162"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            356..1258
                     /locus_tag="AC159P_12675"
     CDS             356..1258
                     /locus_tag="AC159P_12675"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HIR3696347.1"
                     /translation="MLAQETPPTPTVTLSVTATDKKGRVVRDLKREDFRVFEEGAEQK
                     ITHFSKDESPISYGLVVDNSGSLKNLLPVVVATGKAFVGDNRAGDETFIVRFAASDSI
                     HVTQDFTPDKAALKESLDAMYVEGGQTAVIDALYLSADKLSKHERGGPPGRRALILLS
                     DGEDRDSHYRLEDLLKLLRRSDVPVFCVGFVGMLDDEHGFIRPSRREQATALLKRLAA
                     ETGGQAYFPKNNAELQDAFNEVQLHLRERHAIGYTPAPVAGAKARRKVEVKVVEAPGR
                     GKLQAVALPRFVGPKSEGDARRND"
     gene            1339..2307
                     /locus_tag="AC159P_12680"
     CDS             1339..2307
                     /locus_tag="AC159P_12680"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HIR3696348.1"
                     /translation="MRRTRTRTLLCGLALMFIVWPAAAQDKAGKEKEKGKEKAAEQPF
                     EVRLNVTVLDAAGRRVTDLRAEDFRISEDGAAQQVKHFARKEGPPVFGLVVDNTGSLR
                     ELITAVVGFGKLIVEGSDPRGEIFVERFVSSDQITLMQDFTANKAALTDAFDRMYVEG
                     GQSAITDALYLGAEHLAKRKDEAQPPRRRALVLITDGEDRFNINKPAQLVAKLREADV
                     QVFVVALTKAAKPQVVSPDRAIAYMNRIALESGGALWLPDKKSDMVGVAREVILELNA
                     PYTLGFTPANQKRDGSTRSLTVAVPDGPDGGPRRVVVRGSYTAPKK"
     gene            2527..>3624
                     /locus_tag="AC159P_12685"
     CDS             2527..>3624
                     /locus_tag="AC159P_12685"
                     /inference="COORDINATES: protein motif:HMM:NF016289.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HIR3696349.1"
                     /translation="MWRGFIAALLTLSLVTPAALAQQGGAATAAQPAAAAAAAALTAA
                     EREALARVQVETVREVTTALSSKEMEGRGTAQPGGERAAQYLADRFKKLGLKPLGDGG
                     TYLQAVKFKSAQVLPETSLKAGDATLKHGADFVMPPPFTYDEADATGGIVFVGYGVTS
                     EELKRDDLAGLDLKGKIVVTLSGRPSNVDAATWQRANAQKVPTLFMRGIAGLVIAGVG
                     SPQQPFSVITNYLSRRQVGLASAPVPPMKLPPVLLASDAAVEKLFAGSGTTYAQALAK
                     AKTGEAVSRELGKEATLHVRVKREEGAGSNVVAVLEGSDAKLKEEAIVYSAHYDAYGI
                     NPAGVIFPGAADNALGTSMIVSIAEALSKTTP"
BASE COUNT          658 a         1324 c         1141 g          501 t
ORIGIN      
        1 gcaccgtcac gctcgacatc gacccgacga ccggcctcct cgccgccgag acctgccccg
       61 tcgtccgcac caagactttc cagatcggca ccgagccgcg ccgccgctgc ggcccgcagt
      121 tccacaccgg gcgcgacatc atcccttcgg agacgcgccc gcgccgcgtc tcgccttgaa
      181 gcccgtgcgt gaagattcta cggcccatcg ttcagccgcc gcgcccgatc tcaatcgtcc
      241 gaaaggaggc atctctcacg tggcaaatcc tcgtcgcccc cgcggactcc tgccgcgcgc
      301 ggctgtagct tgactggcgc tcctcgtcgc accccgcctc cgtcaacctt ccgtcgtgct
      361 cgcgcaggag acgccgccca cgccgacggt gacgttgagc gtcaccgcca cggacaagaa
      421 gggccgcgtc gtgcgcgact tgaagcggga ggacttccgc gtctttgagg agggcgccga
      481 gcagaagatc acgcacttct cgaaggacga gtcgcccatc agctacggcc tcgttgtgga
      541 caactccggc tcgctcaaaa acttgctccc ggtcgtcgtc gccaccggca aggctttcgt
      601 cggcgacaac cgcgcgggcg acgagacctt catcgtccgc ttcgcggcca gcgacagcat
      661 ccacgtgacg caggacttca cccccgacaa ggccgcgctg aaggagtccc tcgacgcgat
      721 gtacgtcgag ggcggccaga ccgccgtcat cgacgccctc tacctctccg ccgacaaact
      781 gtcgaagcac gaacggggcg ggccgccggg ccgccgcgcg ctgatcctcc tgtcggacgg
      841 cgaagaccgc gacagccatt accggctcga agacctcttg aagctgctcc gccggagcga
      901 cgtgccagtc ttctgcgtcg gcttcgtcgg catgctcgac gacgagcacg gtttcatcag
      961 accgagcagg cgcgaacagg ccacggcgct cctgaagcgc ctcgccgccg aaacgggcgg
     1021 gcaggcgtac ttcccgaaga acaacgccga gttgcaggac gccttcaacg aggttcagct
     1081 ccacctgcgc gagcgacacg ccatcggcta cacgcccgcg ccggtcgccg gggcgaaggc
     1141 ccgccgcaag gtcgaggtga aggtggtcga ggcgccgggc cgcggaaagc tacaggctgt
     1201 cgcgctgccg cggttcgtcg ggccgaagtc ggaaggcgac gcgcggagga atgactgacc
     1261 gcccgccctc cgttataatc ccaccgcacg ccgctcgcct cccccacaag tcaaggccaa
     1321 ggcacgaagg aagaaacgat gcgacgcaca cgaacgcgca cactgctctg cggtctcgcg
     1381 ctgatgttta tcgtctggcc cgccgccgcg caggacaagg ccgggaagga aaaagagaag
     1441 gggaaagaga aggccgccga acagccgttc gaggtgcgcc tgaacgtgac ggtgctcgac
     1501 gccgccgggc gccgcgtcac ggacttgcgc gccgaggact tccgaatctc ggaggacggc
     1561 gccgcgcagc aggtcaagca cttcgcgcgc aaagagggcc cgcccgtctt cggcctcgtc
     1621 gtcgataaca ccggctcgct ccgcgagctg ataaccgccg tcgtcggctt cggcaaactc
     1681 atcgtcgagg gcagcgaccc gcgcggcgaa atcttcgtcg agcgcttcgt cagcagcgac
     1741 cagatcacgc tgatgcagga cttcaccgcg aacaaggcgg cgctcacgga cgcgttcgac
     1801 aggatgtacg tcgagggcgg gcagagcgcc atcaccgacg ccctctacct cggggccgag
     1861 cacctggcga agcgcaaaga cgaggcgcag cccccgcgcc gccgcgcgct cgtcctcatc
     1921 acggacggcg aagaccgctt caacatcaac aagccggcac aactcgtcgc caaactgcgc
     1981 gaggcggatg tgcaggtctt cgtcgtcgcg ttgacgaagg ccgccaagcc gcaagtcgtc
     2041 tcgcccgaca gggccatcgc ttacatgaac cgcatcgcgc tcgaatccgg cggcgcgctg
     2101 tggctgcccg acaagaagtc cgacatggtg ggcgtcgcca gagaggtgat actggaactc
     2161 aacgcgccct acaccctcgg cttcacgcct gccaaccaga agcgcgacgg ctcgacccgc
     2221 agtctcacgg tcgccgtccc cgacgggccg gacggcgggc cgcgccgcgt cgtcgtgcgc
     2281 ggcagctaca ccgcgccgaa gaaatgatga gtgcggaatg atgaatgatg aataaaagac
     2341 gggccgaggg atgaaaggca ccgtgcgtct cgtccttcgt ttatcatcca tcgcttagcc
     2401 gcaaaaattt ccccgccccc gctcggggcg gcaaccattc tttcacgcgc aacgtataca
     2461 cacgtccgca taagtccgac tgaattggag atcaaccgca tgcgtccatc gtaccgaacg
     2521 gaaatcttgt ggcgtggatt tatcgccgcg ctcctgaccc tctcactcgt caccccggcg
     2581 gcgctcgcgc agcagggcgg ggcggcgacc gccgcgcaac ccgccgccgc cgccgccgcg
     2641 gcggcgctca ccgccgccga gcgcgaggcg ctcgcccgcg tccaagtcga gaccgtccgc
     2701 gaagtcacca ccgcgctttc gtccaaagag atggaggggc gcggcaccgc ccagccgggc
     2761 ggcgagcgcg ccgcgcagta cctcgccgac cgcttcaaga agctcggcct caaaccgctc
     2821 ggcgacggcg gcacatacct tcaggccgtc aagttcaagt ccgcgcaggt gctccccgag
     2881 acctcgctca aggcgggcga cgccacgctc aagcacggcg cggacttcgt catgccgccg
     2941 cccttcacct acgacgaggc ggacgccacg ggcggcatcg tcttcgtcgg ctacggcgtc
     3001 acctccgagg agctgaagcg cgacgacctc gcgggcctcg acctcaaggg caagatcgtc
     3061 gtcacgctga gcggtcgccc gtcgaacgtg gacgcggcca cctggcagag ggccaacgcg
     3121 cagaaggtgc ccacgctctt catgcgcggc atcgcgggcc tcgtcatcgc gggcgtcggc
     3181 agcccgcagc agcccttctc cgtcatcacc aactacctct cgcgccgtca ggtcgggctg
     3241 gcgagcgcgc ccgtcccgcc gatgaagctg ccgcccgtgc tgctcgcttc ggacgcggcg
     3301 gtcgagaagc tgttcgccgg ctcgggcacg acctacgcgc aggcgctcgc aaaagccaag
     3361 accggcgaag cggtctcgcg cgagttgggc aaagaggcga cgctccacgt ccgcgtcaag
     3421 cgcgaggagg gggcgggcag caacgtcgtc gccgtgctcg aaggctccga cgcgaagctc
     3481 aaggaggagg ccatcgtcta ctccgcgcac tacgacgcct acggcatcaa cccggcgggc
     3541 gtgatcttcc ccggcgccgc cgacaacgcg ctcggcacgt cgatgatcgt ctccatcgcc
     3601 gaggcgctct cgaagaccac gccg
//
LOCUS       DBNGOH010000650         3272 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4127398, whole genome shotgun sequence.
ACCESSION   DBNGOH010000650 DBNGOH010000000
VERSION     DBNGOH010000650.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3272)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3272)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3272
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4127398"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2967)
                     /locus_tag="AC159P_12690"
     CDS             complement(<1..2967)
                     /locus_tag="AC159P_12690"
                     /inference="COORDINATES: protein motif:HMM:NF040048.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6298 domain-containing protein"
                     /protein_id="HIR3696350.1"
                     /translation="MQHPNNKSTAATVRRAHVVACVLFVAATCGLPFMRATAPTVTAR
                     DDAATAIDFSHAGYGGGGVPLPSVAEVVRVRPSGGDDTALLQAALDHVASLPPRADGF
                     RGALLLTAGRFEVAGRLRMRVSGVVLRGSSGGGRRTTVVATGTGRRTLIEVGGDGAPA
                     TAQPVRVADETVPAGGRALTLESVEGLAAGDRVVVRRPSTAEWIAALGMNRAAGAFAD
                     QRVHWQPGSRDLVWDRTVVEVDAGRKRLTLDAPVTTALEQRYGGGEVAKVVSGETVKR
                     VGVEGLVLESEFERANPRDEEHAWLAVALDHVEDAWVRGVVARHFAGSAVRVGQRARR
                     VTVEDCRNEQPVSEVGGYRRQSFLVEGQQVLVRRCEAEGGLNDFAVGLLAAGPNVFLD
                     CTARGALGASGSFESWASGVLYERVRIEGAPLRLTKDEARAQGGGWTAANSVVWNSEA
                     KEIEARGPEGAVNVVRETPQPLYGTQLIKRRGKQSKIVKEAWQTAPPKKVPEFEFKKA
                     ASAKSKSAATRLPLKIVNGRFVVGGRVVWGGMLNGAWWKGQASPAVARRAGGVSITRF
                     VPGRVGPGLTEDLPALAARMVEEGMPFFDGVPGLWYDRRRDDHTVAERADADVWAPFY
                     EMPWARSGRGKAWDGLSRFDLARFNPWYFGRTREFARLAEQHGLVYYHNLYNTHNLLE
                     TTAHWVDFPWRPANNVNDTGLPEPPPVEGGNNVHVANRFYSTEHAGLRALHRAYIRYN
                     LEQLGASPNVVLILGFQFAGPLAFQRFFHDTLAEWERETGRRARVALITSKDITDAIL
                     ADPARARQVAVIDTRYWQYRPDATLWAPNGGQNLAFREMITRDFGRAGDAPPPTTPQQ
                     LYRQVREYRDRFPDKAVVAWHGGVGPLPILMAGGAQALMRNPAAGQSQGADPDTTPLD
                     AFVREHLSAVLMKMTPRDNLLADPARNWCLADERGEAVLLYSLEGDAIDFAAPLPRKS
                     YTGVWFDPRTGKTTPAA"
     gene            complement(2986..>3272)
                     /locus_tag="AC159P_12695"
     CDS             complement(2986..>3272)
                     /locus_tag="AC159P_12695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007364132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3696351.1"
                     /translation="EALGGVLRHGILLEREAETVTDSLRALLLEGEGYAVKVFEFVST
                     EHTRKNTMITAVRGEDKVDRDAAVTRFRRLKEFYGIREQRLEKLLRESPR"
BASE COUNT          447 a         1211 c         1097 g          517 t
ORIGIN      
        1 cgcggcgggc gtcgtcttgc cggtgcgcgg gtcgaaccac acgccggtgt aactctttcg
       61 cgggagcggg gcggcgaagt cgattgcgtc gccttcgagc gaatagagaa ggactgcttc
      121 gcccctctcg tccgcgaggc accagttgcg cgcggggtct gcgagtagat tgtcgcgcgg
      181 cgtcatcttc atcagcacgg cggagaggtg ttcgcgcacg aaggcgtcga gcggcgtggt
      241 gtccgggtcg gcgccctgcg actgccccgc cgcagggttg cgcatgagcg cctgcgcgcc
      301 gcccgccatc aaaatcggaa gcgggccgac gccgccgtgc cacgcgacga cggccttgtc
      361 ggggaagcgg tcgcggtact cgcgcacctg ccggtagagc tgctgcggcg tggtcggcgg
      421 gggcgcatcg cccgcgcgcc cgaagtcccg cgtgatcatc tcgcggaagg cgaggttctg
      481 gccgccgttc ggcgcccaga gcgtcgcgtc cggtcggtat tgccagtagc gcgtgtcgat
      541 gacggcgacc tgccgcgcgc gcgcggggtc tgcgaggatc gcgtcggtga tgtccttgct
      601 cgtgatgagc gcgacgcgcg cacggcgccc ggtctcgcgc tcccactctg caagggtgtc
      661 gtggaagaac cgctggaagg cgagcggccc cgcgaactgg aagccgagga tgaggacgac
      721 gttgggcgac gcgccgagct gttcgaggtt gtagcggatg taggcgcggt gcagcgcgcg
      781 caagcccgcg tgctcggtgc tgtagaaccg gttggcgaca tggacgttgt tgcccccctc
      841 gacgggcggc ggctcgggca gcccagtgtc gttgacgttg ttggcgggcc gccacgggaa
      901 gtctacccag tgagcggttg tttcgagcag gttatgcgtg ttgtagaggt tgtggtagta
      961 gacgagtccg tgctgctccg cgaggcgcgc gaactcgcgc gtgcgcccga agtaccacgg
     1021 gttgaagcgc gccagatcga agcggcttaa accgtcccac gccttgcccc tgccgctgcg
     1081 cgcccacggc atctcgtaga agggcgccca cacgtccgcg tccgcgcgct ctgcgacggt
     1141 gtggtcgtcg cgccgccggt cgtaccacag ccccggcacg ccgtcgaaga agggcatccc
     1201 ctcctcgacc atccgcgcgg ccagcgccgg caggtcttcc gtcagaccgg ggccgaccct
     1261 gccgggcacg aaccgcgtga tgctcacgcc ccccgcgcgc cgcgcaaccg caggcgatgc
     1321 ctgccccttc caccacgcgc cgttcagcat cccgccccac acgacgcgac cgcccacgac
     1381 gaaacgcccg ttcacgatct tcagcgggag acgcgtcgcc gcgctcttcg atttcgcgga
     1441 agcggctttc ttgaattcga actccggcac cttcttcggc ggcgccgtct gccacgcctc
     1501 tttgacgatc ttggactgtt tgccgcggcg cttgatgagc tgcgttccgt agagcggctg
     1561 cggcgtctcg cgcacgacgt tcacggcgcc ctccggcccg cgcgcttcga tctccttcgc
     1621 ctccgaattc cagacgacgg agttcgccgc cgtccacccg ccgccctgcg ctcgcgcttc
     1681 gtccttcgtg agccgcaggg gcgcgccttc gatgcgcacg cgctcgtaga gcacgcccga
     1741 cgcccagctc tcgaacgagc cgctcgcgcc gagcgccccg cgggccgtgc aatcgagaaa
     1801 gacgttcggc cccgccgcga gcaggccgac ggcgaagtcg ttcagcccgc cttcggcctc
     1861 gcagcggcgc acgagcacct gctgcccttc gacgaggaaa ctctgtcgcc ggtagccgcc
     1921 gacctccgac acgggctgct cgttgcggca gtcttcgacc gtcacgcggc gcgctcgctg
     1981 tccgacgcgc acggcggaac ccgcgaagtg ccgcgccacg acgccgcgca cccacgcgtc
     2041 ttccacatgg tcgagcgcaa ccgcgagcca cgcgtgctcc tcgtcgcgcg ggttcgcccg
     2101 ttcgaactcg ctttcgagca ccagcccctc gacgccgacg cgcttcaccg tctcgcccga
     2161 cacgaccttc gccacctcgc cgccgccgta gcgctgctcg agtgccgtcg tcaccggggc
     2221 gtcgagcgtg agtcgtttcc tcccggcgtc aacttcgacg acggttctgt cccacacgag
     2281 gtcacgcgag ccgggctgcc agtgcacgcg ctgatccgcg aaggcgccgg cggcgcggtt
     2341 catcccaagc gcggcgatcc actccgccgt gctcggtcgc cgcaccacga cgcggtcgcc
     2401 cgccgccagc ccttcgacgc tctccaacgt caacgccctt ccgcccgcgg gcaccgtctc
     2461 gtctgccacg cggacgggct gcgcggtcgc gggcgccccg tcaccgccga cctcgatcag
     2521 cgtgcggcgg cccgtgcccg tggcgacgac ggtcgtgcgc cttccccctc ccgaactccc
     2581 gcgcagcacg acgccgctca cgcgcatgcg gagccggccc gcgacctcga agcgaccggc
     2641 ggtcaacagc aacgccccac ggaacccgtc cgcacgcggt ggcaggctcg ccacgtgatc
     2701 gagcgcggcc tgtagcagcg ccgtgtcgtc gcccccgctc ggcctgaccc tgacgacctc
     2761 agccaccgac ggcagcggca cgccgcctcc accgtaaccc gcgtgcgaga agtcaatcgc
     2821 cgtcgccgcg tcgtcgcgcg cggttacggt cggggccgtg gcccgcatga aaggaaggcc
     2881 gcacgtcgcg gccacgaaga ggacgcacgc gacgacgtgt gcgcgccgca cggtcgccgc
     2941 agtgctctta ttgttcggat gttgcaatgg cgttctcccg tgccgtcatc gcggggactc
     3001 gcgcaagagc ttttcgagcc gctgctcgcg gatgccgtag aactctttga gccgccggaa
     3061 ccgtgttacg gcggcgtccc tgtcaacttt atcctctccg cgcaccgcgg tgatcatcgt
     3121 gttcttgcgg gtgtgctctg tggagacgaa ctcgaagacc ttgacggcgt agccttcgcc
     3181 ttcgagcagc agcgcgcgca aagagtcggt gacggtctcg gcctcgcgtt cgagcaggat
     3241 gccgtggcgc agcacgccgc cgagcgcctc gg
//
LOCUS       DBNGOH010000651         2077 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1746440, whole genome shotgun sequence.
ACCESSION   DBNGOH010000651 DBNGOH010000000
VERSION     DBNGOH010000651.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2077)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2077)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2077
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1746440"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1742)
                     /locus_tag="AC159P_12700"
     CDS             complement(<1..1742)
                     /locus_tag="AC159P_12700"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012845099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 protein"
                     /protein_id="HIR3696352.1"
                     /translation="MSTKIDRRNFIKATAAAGATALVAPTVAGASRAEPAGAQGNAAG
                     ARPLTSYDGQARELLSRMTLEEKVGQMTQPEQDALKELDDIRTYFVGSLLSGGNSDPK
                     KGGVADNSLQAWTDMYDRYQTIAMKTRLGIPILYGVDAVHGHNNVIGAVVFPHNIGLG
                     CTRSARLVEEAARVTAEEVRATGIQWTFAPCVTVPRDERWGRTYEGFGESPELAATLG
                     AAAVRGFQRGDLAHPLSVLACAKHYVGDGGTVIGTGSFGTGDGKKPLLDQGDTRLGER
                     DLRRIHMPGYPATVKAGVGSIMPSYSSWNGVKVSGSKQLLTDILKHELRFEGFLISDY
                     NAIDQITKDYKQAIAVSVNAGMDMVMVPHRYREFFDLLVANVKEGAVPMSRIDDAVVR
                     ILRVKFAMGMMDKSRSQLADRKLHNTFGSAAHREAARECVRASLVLLKNNRRALPLKK
                     TARHIHVAGKSADDIGNQCGGWTIEWQGKSGAVTTGGTTILQAIRNAVSKGTRVTHSK
                     DGTGAAGADIAVVVIGETPYAEGDGDREELSLAAEDVAAVDNLKRAGVPVVAVLVTGR
                     PLILEPVLARCDAFV"
     gene            complement(1811..>2077)
                     /locus_tag="AC159P_12705"
     CDS             complement(1811..>2077)
                     /locus_tag="AC159P_12705"
                     /inference="COORDINATES: protein motif:HMM:NF020122.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Beta-galactosidase C-terminal domain"
                     /protein_id="HIR3696353.1"
                     /translation="GLQEKVLLEVLNRAGLAGPDQQLTAPVRVKHGVGGAGKNLHYYL
                     NYSSRSQTFNYPYADGTDLLARKAIARGRPVTLAPWDLVIVEEK"
BASE COUNT          276 a          699 c          719 g          383 t
ORIGIN      
        1 acgaaggcgt cgcaacgggc gagcacgggt tcgaggatga gcgggcggcc cgtgacgagc
       61 acggccacga cgggaacgcc cgcgcgcttc agattatcga ccgccgccac gtcctccgcc
      121 gcgagcgaga gttcttcgcg gtcgccgtcg ccctcggcgt aaggcgtttc gccgatgacg
      181 acgacggcga tatcggcgcc cgccgcgccc gtcccgtcct tcgagtgtgt cacgcgcgtc
      241 cctttcgaga cggcgttccg gatggcctga aggatggtcg tcccgcccgt ggtcacggcg
      301 ccgctcttgc cctgccactc gatggtccag ccgccgcact ggttgccgat gtcgtcggcg
      361 ctcttgcccg cgacgtggat gtgccgggcc gtcttcttca gggggagcgc ccgccggttg
      421 ttcttcaaca gcaccagcga cgcgcgcacg cactcgcgtg ccgcctcgcg gtgcgccgcc
      481 gagccgaacg tgttgtggag cttgcggtcg gcgagctgcg agcggctctt gtccatcatc
      541 cccatcgcga acttgacgcg gaggatgcgc acgacggcgt cgtcgatgcg cgacatcggc
      601 accgcgccct ctttgacgtt ggcgacgagc aggtcgaaga actcacggta tctgtgcggc
      661 accatcacca tgtccatccc ggcgttgacc gagacggcga tggcctgctt gtagtccttc
      721 gtgatctggt cgatggcgtt gtagtcggag atgaggaagc cttcgaagcg gagttcgtgt
      781 ttgaggatgt cggtcagaag ctgcttgctg cccgacacct tcacgccgtt ccaactgctg
      841 tacgaaggca tgatggagcc gacgccggcc ttgacggtcg cggggtagcc gggcatgtgt
      901 atgcgccgca ggtctcgctc gccgagccgc gtgtcgccct gatcgagcag cggctttttg
      961 ccgtcgcccg tgccgaagct gcccgtgccg atgacggtgc cgccatcgcc gacgtagtgc
     1021 ttggcgcagg cgagcacgct cagagggtgc gcgaggtcgc cgcgctggaa gccgcgcacc
     1081 gccgccgcgc ccaacgtagc ggccagctcg ggcgactcgc cgaagccctc gtaggtgcgg
     1141 ccccagcgct cgtcgcgcgg gacggtgacg cagggcgcga acgtccactg gatgccggtg
     1201 gcgcgcacct cctcggcggt gacgcgcgcg gcctcttcga caagccgcgc gctgcgcgtg
     1261 caccccagcc cgatgttgtg cgggaagacg acggcgccga tgacgttgtt gtgcccgtgc
     1321 acggcgtcca cgccgtagag gatggggatg ccgagccgcg tcttcatcgc aatcgtctgg
     1381 tagcggtcgt acatgtccgt ccacgcctgg aggctgttgt cggcgacgcc ccccttcttc
     1441 gggtcggagt tgccgccgct cagcagcgag ccgacgaagt aggtgcggat gtcgtccagc
     1501 tctttgaggg cgtcctgttc gggctgcgtc atctgcccga ccttctcttc gagcgtcatc
     1561 cgcgagagca gctcgcgcgc ctgcccgtcg taagaggtga gcgggcgcgc gcccgccgcg
     1621 ttcccctgcg cgcccgcagg ctccgcgcgg ctggcgcccg cgaccgtggg cgcgacgagg
     1681 gccgtggccc cggcggctgc cgtcgccttg atgaaattgc ggcggtcgat cttcgtgctc
     1741 atatcggctg tcgctccttc gatacgtttt atgatctccg gcgtccggtt tccggcggca
     1801 cgccgcccgt tcatttctcc tcgacgatca ccaagtccca gggggcgagc gtgacgggtc
     1861 gccctcgcgc gatggctttg cgcgcgagca agtccgtgcc gtcggcgtaa gggtagttga
     1921 aggtctggct ccggctcgaa tagttgagat agtaatgcag gttcttcccc gcgcccccga
     1981 cgccgtgctt gaccctgacg ggggcggtga gttgctggtc ggggccggcg aggccggcgc
     2041 ggttcaagac ttcgagcaga accttttctt gcagccc
//
LOCUS       DBNGOH010000652         2235 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4127761, whole genome shotgun sequence.
ACCESSION   DBNGOH010000652 DBNGOH010000000
VERSION     DBNGOH010000652.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2235)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2235)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2235
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4127761"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..765)
                     /locus_tag="AC159P_12710"
     CDS             complement(<1..765)
                     /locus_tag="AC159P_12710"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016228.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR3696354.1"
                     /translation="MKILVTGGSGYLGTHVRSFFGADDFSRRSGFDVTSDYDCEKVAD
                     YDVVIHLAALLDKAEEAAESCFRTNAEGTANLLRRARPGTVFVYASTKDVYGANAEGY
                     EEVPETCPTDYRGQTALEWSKLMGERYVEYYAARLNLRACVFRLSATYARPSEGNEPG
                     FVAHYVESLKRRRPIRLPAQGAPVRDILHVEDFSRACRAFIDSRRRAGLYNLGGGRQN
                     AATLREMIETVSRAVEIEPVFDDAPPPSAPVPFHYVS"
     gene            complement(818..1090)
                     /locus_tag="AC159P_12715"
     CDS             complement(818..1090)
                     /locus_tag="AC159P_12715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017740867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3303 domain-containing protein"
                     /protein_id="HIR3696355.1"
                     /translation="MLYMVVEHFKQQDALPVYRRFRQRGRLAPEGLTYVASWVDENFH
                     RCFQLMETDDRNLLDRWLANWNDLVDFEVFPVMTSREAAERMAPYL"
     gene            complement(1151..2179)
                     /locus_tag="AC159P_12720"
     CDS             complement(1151..2179)
                     /locus_tag="AC159P_12720"
                     /inference="COORDINATES: protein motif:HMM:NF014325.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HIR3696356.1"
                     /translation="MPPVTNHLTGRLRTRLASSLAALVGVVTLGTAGFMLVEGWSLGD
                     SLYMTLMTVTTVGYGPPLPLSAAGRNFSIFFMIVGVGTAGYLLSTAVQALVQSEILAA
                     LGERRRQREMSKLKDHFIICGAGRVGQRIIRELQRAGVAFVIIESDPRKAETLADLHA
                     PLLVRDATLEETLREAGVERARALATCLPDDADNLYVVLLARDLNRKLHIVARAVEEQ
                     AEPKLIRAGASRVVAPTIIGSHRMAQALLKPAIADFMDSITAENLDLGFEQVEVAHAS
                     ALAGRKLRDTTIRSELEVVVAAIRRASGEMIFNPSGETEIMVGDLLIAIGRAESLAEL
                     GKLARGER"
BASE COUNT          358 a          718 c          794 g          365 t
ORIGIN      
        1 cgagacgtag tggaacggca cgggcgcgga cggaggcggc gcgtcgtcga agacgggttc
       61 gatctcgacg gcgcggctga ccgtttcgat catctcgcgg agcgtggccg cgttctgtct
      121 gcctccgccg agattataca acccggcgcg gcggcgcgag tcgatgaagg cgcggcaggc
      181 gcgcgaaaag tcttcgacgt ggaggatgtc gcggacggga gcgccctgcg cgggcaggcg
      241 gatggggcgg cgccgcttca gggattcgac gtagtgcgcg acgaagcccg gctcgttgcc
      301 ctcggacggg cgcgcgtaag tggccgagag gcggaagacg caggcgcgca agttcaggcg
      361 ggccgcgtag tattcgacgt agcgctcgcc catgagcttc gaccattcga gcgccgtctg
      421 cccgcggtag tcggtcgggc acgtctcggg cacctcctcg tagccctcgg cgttcgcgcc
      481 gtacacgtcc ttcgtcgagg cgtagacgaa gaccgtgccg ggccgcgcgc ggcgcaggag
      541 gttggccgtc ccttcggcgt tcgtgcggaa gcagctttcg gcggcctctt ccgccttgtc
      601 gagcagcgcg gcgaggtgga tcaccacgtc gtagtcggcg accttttcgc aatcgtagtc
      661 gctcgtcacg tcgaagccgg agcggcgcga gaaatcgtcc gccccgaaga aactccggac
      721 gtgcgtgccg agatagccgc tcccgcctgt cactagaatc ttcaattttc gaattcgggg
      781 ttcgaagtgc gtcgcgacct tcgagaccgc cccttcgtca aaggtacggg gccattcttt
      841 cggcggcttc ccgcgaggtc atgaccggga acacctcgaa gtccacgagg tcgttccagt
      901 tggcgagcca gcggtcgagc aggttccggt cgtccgtctc catcagttga aaacagcggt
      961 ggaaattctc gtcaacccaa ctcgcgacgt aggtcagccc ctccggggcg agccgcccac
     1021 gctgccggaa gcggcggtag acgggtaagg cgtcttgctg cttgaagtgt tccaccacca
     1081 tgtaaagcat ggccgagtgt agaaactctc tggcgcgctg tccaatgcgt cggcgcccgc
     1141 tgcccggcgc ctaccgctcc ccccgcgcca gcttgcccag ctcggcgaga gattcggcgc
     1201 gaccgatggc gatgagcagg tcgccgacca tgatttcggt ttcgcccgag gggttgaaga
     1261 tcatctcgcc cgaggcgcgg cggatggcgg cgacgaccac ttcgagttcg gagcggatgg
     1321 tcgtgtcgcg tagcttgcgc ccggcgagcg cagaggcgtg cgcgacctcg acctgctcga
     1381 agccgaggtc gaggttctcg gcggtgatcg agtccatgaa gtctgcgatg gccggtttga
     1441 ggagcgcctg cgccatgcgg tggctgccga tgatggtggg cgcgacgacg cggctcgcgc
     1501 ccgcgcggat gagcttcggc tcggcctgct cctcgacggc gcgcgcgacg atgtgcagct
     1561 tgcggttgag gtcgcgcgcc agcagcacga cgtagaggtt gtcggcgtcg tccggcaggc
     1621 aggtggccag cgcgcgggcg cgctcgacgc cggcctcgcg cagcgtctct tcgagcgtcg
     1681 cgtcgcgcac cagcagcggc gcgtgcaggt cggccagcgt ctcggccttg cgcgggtcgc
     1741 tctcgatgat gacgaaggcg acgcccgcgc gttgcagctc gcggatgatg cgctggccga
     1801 cgcgccccgc gccgcagatg atgaagtggt ctttgagctt gctcatctcg cgctgccgcc
     1861 tccgctcgcc gagcgcggcg agtatttcgg actggacgag cgcctgcacg gcggtcgaga
     1921 gcaaataacc ggcggtgccg acgccgacga tcatgaagaa gatcgagaag ttgcgccccg
     1981 ccgccgagag cggcagcggc ggcccgtagc cgacggtcgt caccgtcatc agagtcatgt
     2041 agaggctgtc gccgagcgac cacccctcga cgagcatgaa gcccgccgtg cccagcgtca
     2101 ccacgcccac gagcgcggcc agcgacgagg ccagccgtgt ccgcaggcga cctgtgagat
     2161 gattggttac gggaggcatc gaaccgcacg cgaccgcggg acatcaaaaa agcgtccgaa
     2221 cgttagcgaa aggtg
//
LOCUS       DBNGOH010000653         4292 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4495243, whole genome shotgun sequence.
ACCESSION   DBNGOH010000653 DBNGOH010000000
VERSION     DBNGOH010000653.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4292)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4292)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4292
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4495243"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..546
                     /locus_tag="AC159P_12725"
     CDS             <1..546
                     /locus_tag="AC159P_12725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696357.1"
                     /translation="RSLANMTREAERERLLPAPEPTVEEPRDVFDSTLPQEYLEQIRQ
                     AVGQDFKLLDLRVNEMGLSAKLSTDGESVKEYRRWKHRKNVEGPFDVKIIGDGKVGDN
                     LLEPAAVDLSLVPKLSKEAFERAAFPDSKVDGASIQYPLFRTRGQGPEWTVNLSAKRG
                     EKWEFKYVTFDAKGRFKEVRD"
     gene            735..1880
                     /locus_tag="AC159P_12730"
     CDS             735..1880
                     /locus_tag="AC159P_12730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HIR3696358.1"
                     /translation="MEFELNEEQQQVKASVREFAEGEIAPHVREWDEKQRLPMELFPK
                     MAEQGFMGVIFPEGYGGAGMGYIEYVTIIEELSRVDGSIGISVAAHNSLCSNHIYMFG
                     TEEQKRKYLVPLAQGRHLGAWGLTEPGAGSDASGTRTTAVKRGGAWVVNGSKNFITHA
                     IHGDTCVAVASTDRAMRSKGITAFIFEKGMPGFMPGKKEDKLGLRASETASVIFEDCR
                     VPDENRLGPEGLGFIQAMQILDGGRISIAALALGIAQGAYESALKYSKERKQFGQPIS
                     NFQAIQFKLADMATQIDAARLLTYRAAWLKDSNRKVTKESSMAKLFASEISVKVCEEA
                     IQIHGGYGYTKDYPPEKYWRDSKLCTIGEGTSEIQRLIIAKQILSNV"
     gene            1895..2431
                     /locus_tag="AC159P_12735"
     CDS             1895..2431
                     /locus_tag="AC159P_12735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696359.1"
                     /translation="MTNIFDDEDASSTQGDWPLLHPIEGGFAMSAETTERFNGSDSFE
                     TRVLTALAAINGQLGSINVRLTALEEKVGAIDARLTAVEEKVNAIDARLTAVEQKVGA
                     IEVRLGGLEKKVDALDQKVDALEEKVDARLRETRPIWESVLERLKVMDAKFDAFALDM
                     FEMRAEIILIKKRVPPAA"
     gene            2443..3411
                     /gene="meaB"
                     /locus_tag="AC159P_12740"
     CDS             2443..3411
                     /gene="meaB"
                     /locus_tag="AC159P_12740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HIR3696360.1"
                     /translation="MESSNLVERVLRGEARAVARAISKAEDGAEDAFALMKALFPHTG
                     RGLVVGITGAPGAGKSSLVDRLAALYRRREERVGIVAVDPSSPFSGGAILGDRIRMQT
                     LGLDPGVFIRSMATRGNLGGLARATVDAVAILDAAGFGKIIVETVGVGQDEVEIVKAA
                     DVSVVVLVPGMGDDIQAIKAGIMEIGDVFVINKADRDGVLRTEKELEALLSLATRPDG
                     WEPPIVKTVATENKGVEELAAAIESYRDFQKGAESGRARRASIARWRILELLRERLLA
                     RALGGEGAGEQLDALALEVADKRRDPYSAVEEFIAAGTLGEPRRGE"
     gene            3484..3909
                     /gene="mce"
                     /locus_tag="AC159P_12745"
     CDS             3484..3909
                     /gene="mce"
                     /locus_tag="AC159P_12745"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HIR3696361.1"
                     /translation="MEIAHIGIATPSIEDALKFWRDALGLRVAHTEEVAEQGVRVAML
                     PLGGPNVELLEPTGPDSPVAKFLEKRGPGIHHVAVRVPDIRAALARLETGGARLIDET
                     PRVGAGGCLVAFVHPSSAGGVLLELVEETEDSGGSGHER"
     gene            3916..>4292
                     /locus_tag="AC159P_12750"
     CDS             3916..>4292
                     /locus_tag="AC159P_12750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696362.1"
                     /translation="MNSIKVLLAALFVASSLCTSALAQRTARRPPPTAAAPSTLPAGG
                     INLTAADMALVVDGFGFPPEAVARLAANPEERRAFAKDLRQMLATAEEAKSKGVAARP
                     ETRLQLELARAFIIAQEYFKRREA"
BASE COUNT          788 a         1292 c         1534 g          678 t
ORIGIN      
        1 cgctcgctcg ccaacatgac gcgcgaggcc gagcgcgagc gcctgctgcc ggcgcccgag
       61 ccgaccgtgg aggagccgcg cgacgtgttc gacagcacgc tcccgcagga gtatctcgaa
      121 cagatcaggc aggcggtcgg gcaggacttc aagctgctcg acctgcgcgt gaacgaaatg
      181 ggcctgtcgg cgaagctctc gaccgacggc gagagcgtca aggagtaccg gcgctggaag
      241 catcggaaga acgtcgaggg gccgttcgac gtgaagatca tcggcgacgg caaggtggga
      301 gacaacctgc tcgaacccgc cgccgtcgat ttgtcgctcg tgccgaagtt gtcgaaggag
      361 gcgttcgagc gcgcggcctt cccggactcg aaggtggacg gcgcgtcgat ccagtacccg
      421 ctcttccgca ccagaggcca gggccccgaa tggacggtga acctctcggc caagcgcggc
      481 gagaagtggg agttcaagta cgtcaccttc gacgcgaagg gcaggttcaa agaggtgagg
      541 gattaaagcc ctcgccgcga gcgatggttg actgtcgcgc acgcccctcc acgtttcgca
      601 taagcttccg tctcaaagta gaatgaacgc gaaggcgcgc ggcgtcgtgt cggctggtgc
      661 cggaagtatc cccgctaccg cgcgcttttc cgtgtgcggc aatcttaaat catccgcgag
      721 gggcgttcac tgcaatggag ttcgaactca acgaagagca gcagcaggtc aaggcgagcg
      781 tgcgcgagtt cgcggagggc gagatcgcgc cgcacgtccg cgagtgggac gagaagcaac
      841 gtctgccgat ggagctgttc ccgaagatgg ccgagcaggg cttcatgggc gtcatcttcc
      901 ccgaagggta cggcggcgcg gggatgggct acatcgaata cgtgacgatc atcgaggagc
      961 tgtcgcgcgt ggacggctcc atcggcatct cggtcgcggc gcacaattcg ctctgctcga
     1021 accacatcta catgttcggc accgaggagc agaagcggaa gtatctggtg ccgctcgcgc
     1081 aagggcgcca tctcggcgcg tgggggctga ccgagccggg cgcggggtcg gacgcctcgg
     1141 gcacgcgcac gaccgccgtg aagcggggcg gcgcgtgggt cgtcaacggc tcgaagaact
     1201 tcatcacgca cgccatccac ggcgacacct gcgtggccgt cgcctcgacc gaccgcgcga
     1261 tgcgctcgaa gggcatcacc gccttcatct tcgagaaggg gatgcccggc ttcatgcccg
     1321 gcaagaagga ggacaagctg ggcctgcgcg cgtcggagac ggcgagcgtc atcttcgagg
     1381 actgccgcgt gccggacgag aaccgcctcg gccccgaggg cttgggcttc atccaggcga
     1441 tgcagattct cgacggcggg cgcatctcca tcgccgcgct cgcgctcggc atcgcgcagg
     1501 gcgcctacga gtcggcgctc aagtactcga aggagcgcaa gcagttcggc cagcccatct
     1561 cgaatttcca ggccatccag ttcaagctcg ccgacatggc gacgcagatc gacgccgcgc
     1621 gcctgctgac ctaccgcgcc gcgtggctca aggactcgaa ccgcaaggtg acgaaagagt
     1681 cctcgatggc gaagctcttc gcctccgaga tttcggtcaa ggtctgcgag gaggcgatcc
     1741 agattcacgg cggctacggc tacaccaaag actacccgcc cgaaaaatac tggcgcgact
     1801 ccaagctctg caccatcggc gaaggcacct ccgagattca gcgtttgatt atcgccaaac
     1861 agattctcag caacgtgtga cgacgagcga agttttgacg aacatcttcg acgacgaaga
     1921 tgcttcttca actcaaggag actggccgct cctccacccg atagaaggag gatttgcgat
     1981 gagtgcagag acgaccgaga gattcaacgg cagtgattcg ttcgagacgc gcgtgctaac
     2041 cgcgctggca gcgataaatg gccaactggg ttcgataaac gtgcgtctga ccgcgctcga
     2101 agaaaaagtc ggcgcgatag acgcccggtt gactgcggtg gaagagaaag tgaacgcgat
     2161 agacgctcgc ctcaccgcgg tcgaacagaa agtgggtgcg atagaagtac ggttgggtgg
     2221 gcttgagaag aaagtcgatg cgcttgacca gaaagttgat gcgttagaag agaaagttga
     2281 cgcgcggctg cgggagacgc gaccgatctg ggagtcggtg ctggagcgac ttaaagtgat
     2341 ggacgcgaag ttcgatgcgt tcgcactcga catgttcgag atgcgtgccg agatcatctt
     2401 gatcaagaag cgagtgccgc ccgcggcgtg agtagtgttt tgatggagag ttcgaactta
     2461 gtcgagcgcg tgttgagggg cgaggcgcgg gccgtggcgc gggccatctc gaaggcggag
     2521 gacggcgcgg aggacgcgtt cgcgttgatg aaggcgctgt tcccgcacac ggggcgcggc
     2581 ctcgtggtcg gcatcacggg tgcgccgggc gcgggcaagt cttcgctggt cgataggctc
     2641 gccgcgcttt accgccggcg cgaagagcgc gtcggcatcg tcgccgtcga tccgtcgagt
     2701 ccgttctcgg gcggcgcgat tctgggcgac cggatcagga tgcagacgct cggtctcgac
     2761 cccggcgtgt tcatccgttc gatggcgacg cgcgggaacc tcggcggcct cgcgcgcgcg
     2821 accgtggacg ccgtggcgat actggacgcc gcagggttcg gcaagatcat cgtcgagacg
     2881 gtgggggtcg ggcaggacga ggtggagatc gtgaaggcgg cggacgtgtc ggtggtcgtg
     2941 ctcgtgccgg gcatgggcga cgacatacag gccatcaagg cgggcatcat ggagatcggc
     3001 gacgtgttcg tcatcaacaa ggccgaccgc gacggcgtgc tgcgcacgga gaaggagctg
     3061 gaggcgctgc tcagtctggc gacgaggccg gacgggtggg agccgccgat tgtgaagacg
     3121 gtggcgacgg agaataaggg cgtcgaggag ttggcggcgg ccatcgagag ctatcgtgat
     3181 tttcagaagg gggccgagtc gggccgcgcg cgccgcgctt cgattgcgcg ctggcgcatc
     3241 cttgagctgc tgcgggagcg tctgctggcg cgcgcgctcg gcggcgaagg cgcgggcgag
     3301 cagctcgacg cgctcgcgct cgaagtcgcg gacaagcggc gcgaccctta ctcggcggtc
     3361 gaggagttca tcgccgccgg gacgctcggc gagccgcgcc gcggagaata gcggagagta
     3421 gttgacggca gcccgccacc gacgctcgac atccaaacat tttctggccg gagacacgga
     3481 cacatggaga tcgcgcacat cggaatcgcc acgccgagca tcgaagacgc cttgaagttc
     3541 tggcgcgacg cgctcggcct ccgggtcgcg cacacggagg aggtcgcgga gcagggcgtg
     3601 cgggtggcga tgctgccgct gggcgggccg aacgtcgagc tgttggagcc gaccgggccg
     3661 gactcgcccg tggcgaagtt tcttgagaag cgcggccccg gcatccatca cgtcgcggtg
     3721 cgcgtgccgg acatccgcgc cgcgctcgcg cgcctcgaaa cgggcggcgc gcgcctgatc
     3781 gacgagacgc cgcgcgtggg cgcgggcggg tgccttgtcg cgttcgtcca cccctcgtcc
     3841 gccggtggcg tcctgctcga actcgtcgag gagacggaag actccggcgg gtcggggcat
     3901 gaaaggtaag tagttttgaa ttcgatcaaa gtccttctcg cggctctctt cgtcgcgtct
     3961 tcactgtgca cttcagcgct cgcgcagcga accgcgcgcc gcccgccgcc gacggcggcg
     4021 gcgccgtcaa cgttgcccgc gggcggcatc aacctcacgg cggcggacat ggcgctcgtt
     4081 gtggacggct tcgggttccc gccggaggcg gtcgcgcgtc tcgccgccaa ccccgaggag
     4141 cgcagggcgt tcgccaaaga cctgcgccag atgctcgcca ccgccgagga ggcgaagtcg
     4201 aagggcgtgg cggcgcggcc cgagacccgt ttgcagttgg agctggcgcg cgcgttcatt
     4261 atcgcgcagg agtacttcaa gcggcgcgag gc
//
LOCUS       DBNGOH010000654         3751 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3029177, whole genome shotgun sequence.
ACCESSION   DBNGOH010000654 DBNGOH010000000
VERSION     DBNGOH010000654.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3751)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3751)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3751
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3029177"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1209)
                     /locus_tag="AC159P_12755"
     CDS             complement(<1..1209)
                     /locus_tag="AC159P_12755"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017742576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HIR3696363.1"
                     /translation="MKTNPHVPARLVKVRGTELVAGDSRERYREKIARITLDSMVQFV
                     GLLDAEGTVLEINQVALDAVGIKLSDVEGKPFWTTFWWQVSEEINATLREMIARAAQG
                     EFVRWDTPIYGRAGGKETIIIDASLMPVRDEHSNVVFIAAEGRDITEKKAYEREIARQ
                     REELAELDKLKTQFFANISHEFRTPLTLMMGPLEDALAEAEGLSAANRERLELARRNA
                     QRQLKLVNTLLDFSRIEAGRIEAVFEPTDLAAFTAELASVFRSAIEKAGMKLVVDCPP
                     LPEPVWVDRELWEKIVLNLLSNAFKFTFDGEIEVSLREAGGAVQLAVSDTGTGIPAEE
                     LPRLFERFHRVESSGARSGEGSGIGLALVRELVLLHEGEVSASSEPGVGTTMTVTIPF
                     GRAHRALADPP"
     gene            1456..1743
                     /locus_tag="AC159P_12760"
     CDS             1456..1743
                     /locus_tag="AC159P_12760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696364.1"
                     /translation="MTYQKQTTVGVIPNPTIDFKIEANPVTPGKPLSFTLGWTVKDVN
                     LFQVTANDGPDGTPYVLPVPFSREGTYPVTPHRLDTTYTMQVITKAAPHED"
     gene            1756..2025
                     /locus_tag="AC159P_12765"
     CDS             1756..2025
                     /locus_tag="AC159P_12765"
                     /inference="COORDINATES: protein motif:HMM:NF019126.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HIR3696365.1"
                     /translation="MSSNPSASVTATINLPLPVGTVIAYMGSPQSVDALAPLGWLDCN
                     GVAASSKTYPDLFKVIGTTYGGDGKPDFNLPQLSGAFLRGVDPGG"
     gene            complement(2057..2548)
                     /locus_tag="AC159P_12770"
     CDS             complement(2057..2548)
                     /locus_tag="AC159P_12770"
                     /inference="COORDINATES: protein motif:HMM:NF024816.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3696366.1"
                     /translation="MNAHRKSPKLLVTAALALVAAACLAAAGIRAGREGTRSRPDTTA
                     HARQSTATSGDASAALRVQSLTLRGGQLREAGRYQVALPLLQEALALAEGAFGPDSLE
                     AADVLNQLGMLGKSDRRFDEAEAAFRRALAIYERTLPPSHPKLVTCRENYERLLRELK
                     GRM"
     gene            complement(2864..>3751)
                     /locus_tag="AC159P_12775"
     CDS             complement(2864..>3751)
                     /locus_tag="AC159P_12775"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018974290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent decarboxylase
                     family protein"
                     /protein_id="HIR3696367.1"
                     /translation="AEGLFGAPEVKVVVGAEVHPTLLKALGMLGLGRKRLTTVPTDGQ
                     GRMRADALPALSGSEPTIVCIQAGNVNTGAFDPAAEICAVAHAAGAWVHIDGAFGLWA
                     AASPRRAHLAAGVAEADSWATDAHKWLNVPYDSGLAFVRRRETLRAAMAISAAYLPEG
                     EMREPIQYTPESSRRARGVEVWAALRSLGREGLADLVERNCAYAARFAEGFRAAGYEV
                     LNEVLLNQVVVSFGDAEATRRVVTGVQADGTCWCGGTVWQGQAAMRVSVSSWATTEED
                     VERSLEAILRVASAEAGKK"
BASE COUNT          570 a         1329 c         1192 g          660 t
ORIGIN      
        1 gggcgggtcg gcgagcgcgc ggtgcgcgcg gccgaacggg atcgtcaccg tcatcgtggt
       61 gccgaccccg ggctcgctgg acgcgctgac ctcgccctcg tggagcagca cgagctcccg
      121 gacgagcgcg agaccgatgc cgctgccctc gccggagcgc gcgcccgagc tctccacgcg
      181 gtggaagcgc tcgaacaggc gcggcagctc ctccgccggg atgcccgtcc ccgtgtcgct
      241 caccgcgagc tgcaccgccc cgccggcctc gcgcaggctg acttcgatct ccccgtcgaa
      301 ggtgaatttg aaagcgttcg agagcaggtt gaggacgatc ttctcccaca gctcgcggtc
      361 aacccagacg ggttcgggca gcggcgggca gtccacgacc agcttcatcc cggccttctc
      421 gatggccgag cggaagacgc tggcgagttc cgccgtgaag gcggcgaggt cggtcggctc
      481 gaagacggcc tcgatgcgcc cggcctcgat acgcgagaag tccagcagcg tgttgacgag
      541 cttgagctgg cgctgggcgt tgcggcgcgc cagttccagg cgctcgcggt tggcggcgga
      601 caaaccttcc gcctcggcca gcgcgtcttc gagcggcccc atcatcaagg tcagcggcgt
      661 gcggaactcg tggctgatgt tggcgaagaa ctgcgtcttg agcttgtcga gttcggccag
      721 ctcctcgcgc tggcgcgcga tctcgcgctc gtacgccttc ttctcggtga tgtcccgccc
      781 ctcggcggcg atgaacacga cgttgctgtg ctcgtccctg accggcatga gcgaggcgtc
      841 gatgatgatc gtctccttgc cgcccgcgcg cccgtagatg ggcgtgtccc agcgcacgaa
      901 ttcgccttgc gccgcgcgcg cgatcatctc tctcaaagtc gcgttgatct cctccgagac
      961 ctgccaccag aaggtcgtcc agaagggctt cccctcgaca tcggagagtt tgatccccac
     1021 ggcgtcgagc gcgacctgat tgatttcgag caccgtgccc tctgcgtcca gcaggccgac
     1081 gaactgaacc atcgagtcca gcgtgatgcg cgcgatcttc tcccgatacc gttcccgaga
     1141 gtcgccggcc acaagctcgg tgccgcggac tttgacgagg cgggcgggca cgtgcggatt
     1201 ggtcttcatc cgaagatggg tctccgttct tgctcttcgc gtgtggggcc gcaacctccg
     1261 acttcggcac gatccgtcgg tcgtgtcgcg gcaaccgggt ctccgattat actcgtcccg
     1321 ctctcgtccc tcatgactgc ctgaagcgct ccccgtgaag cataacacct ccccttcgag
     1381 tggcggcact gcacgcccga agtcgtcctt ttcacccttc aggcgaagtt gaccgttccg
     1441 gggcagtcgt cgccgatgac ttaccagaag cagacgaccg tcggtgtgat acccaatccg
     1501 accatcgatt tcaagatcga ggccaacccc gtcacccccg gcaagccttt gagcttcacc
     1561 ctcggttgga cggtcaagga cgtgaatctt tttcaagtga cagccaacga cggcccggac
     1621 ggcaccccct acgttctgcc ggtgccgttc tcgcgggaag gcacttaccc ggtcactccg
     1681 catcgccttg acaccaccta cacgatgcag gtcatcacga aggcagcccc gcacgaagat
     1741 taaggagaaa acgacttgtc atccaacccc agtgcttcag tcaccgccac gatcaacctg
     1801 ccccttcctg tcggaaccgt catcgcctac atgggatcgc cgcaatccgt ggatgctctg
     1861 gccccgctcg gctggctgga ctgcaacggc gtcgcggctt ccagcaagac ctatcccgac
     1921 ttgttcaagg taatcgggac cacctacggg ggcgacggca agcctgattt caacctgccg
     1981 cagttgtcgg gggccttcct gcgcggggtc gatccgggcg gctgattgcc gtagcgggcg
     2041 ccggtcttcc cggccttcac atgcgacctt tcagctccct tagcagccgt tcgtagttct
     2101 cccgacaggt cacgagcttc gggtgcgacg gcgggagcgt gcgctcgtag atggcgaggg
     2161 cgcggcggaa cgcggcttcc gcttcgtcga agcgcctgtc ggacttgccg agcatgccga
     2221 gctggttgag gacgtcggcg gcttcgagcg agtcggggcc gaaggcgcct tcggccagcg
     2281 ccagcgcttc ctggaggagc ggcaacgcca cctgataacg gcccgcttcg cgcagttggc
     2341 cgccgcgcag ggtcagagac tggacgcgaa gtgccgccga cgcgtcgccg cttgtggccg
     2401 ttgactggcg tgcgtgcgcg gtggtgtcag ggcgcgaccg cgtcccttcg cgccccgcgc
     2461 ggatgcccgc cgccgcgagg caggcggcgg cgacgagcgc gagcgcggct gttacgagca
     2521 gcttcggcga tttcctgtgg gcgttcatgt ccgtgcccct ttcgcccgac cttcgatctc
     2581 gacggattgc tccggccaac gccggcatta cgatgggcgc ggtttcgcgt cctcgaaacc
     2641 gccgagtagg cttgcttcgt cgggtgtgag gagactacct ctggggcggc gcggctgtac
     2701 ttaggaaccc actgattttt tctgaaggag tttctgaagg cggcatgtcc gggcaggaac
     2761 agcacttgta ccgacaggcg aagcacttgg agggcacgct tccgagccgg ctcggcggtg
     2821 tgtcgatgcg ccggtcgcgc gcttcgagca agctcaccgg cacttacttt tttccggcct
     2881 ccgccgacgc gacgcgcagg atcgcttcca aactccgttc cacgtcctcc tccgtcgtgg
     2941 cccaggagga gacgctcacg cgcatcgccg cttgcccctg ccagaccgtc ccgccgcacc
     3001 aacaagtgcc gtcggcctgc acccccgtga cgaccctccg cgtcgcctcc gcatccccga
     3061 acgagaccac gacctgattg agcaggacct cgttgaggac ctcgtagccc gcggccctga
     3121 agccttcggc gaagcgcgcc gcgtacgcgc agttgcgctc gacgaggtcg gccagccctt
     3181 cgcgcccgag cgagcgcagg gccgcccaca cctcgacgcc gcgcgcccgc cgcgaagact
     3241 ccggcgtgta ctgtatcggc tcgcgcatct ccccctcggg caggtaggcg gcgctgatcg
     3301 ccatcgcggc cctcagcgtc tcgcggcggc ggacgaaggc cagcccgctg tcgtacggca
     3361 cgttcagcca cttgtgcgcg tccgtcgccc acgagtccgc ctccgcgacg cccgccgcca
     3421 gatgcgcccg cctcggggaa gcagccgccc acaggccgaa ggctccgtcg atgtgcaccc
     3481 acgcgcccgc cgcgtgtgcg accgcacata tttccgcggc ggggtcgaag gcccctgtgt
     3541 tcacgttccc ggcctgaata caaacgatgg tcggttcgga gccggacaag gcggggagcg
     3601 cgtccgcccg catcctgccc tgcccgtcgg tcggaaccgt tgtcagccgt ttccgcccga
     3661 ggcccaacat cccgagggcc ttcagcagcg tgggatgtac ttcggcgccg acgacgactt
     3721 tcacctccgg cgcgccgaac agcccttcgg c
//
LOCUS       DBNGOH010000655         2607 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3029364, whole genome shotgun sequence.
ACCESSION   DBNGOH010000655 DBNGOH010000000
VERSION     DBNGOH010000655.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2607)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2607)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2607
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3029364"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..828)
                     /locus_tag="AC159P_12780"
     CDS             complement(<1..828)
                     /locus_tag="AC159P_12780"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014425.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArnT family glycosyltransferase"
                     /protein_id="HIR3696368.1"
                     /translation="MLILGLQLALSVRRESQTWDEGNHIYAGYMSWRRADFGLNPEHP
                     PLVKLLATAPLLPLPLKAPEPQDRYFKEAAFLGGKDFLYRNDADMILFRTRMAAATLT
                     LLLALLVFVATREMFGTGAAFIALALLAFEPNLLAHGALVTTDAGISCFMLATIYAFY
                     RFAKAPSVWRVALVGVSAGLALAVKHTGIFVFPMLFVLAVCEVLRHRRAAKGDGTTAT
                     KESGKQALKFAAALAAIVVIAVAVLWSFYGFRYRARPDGLQLNPPLAEYAQGLKPHQG
                     "
     gene            complement(999..1559)
                     /locus_tag="AC159P_12785"
     CDS             complement(999..1559)
                     /locus_tag="AC159P_12785"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014081.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR3696369.1"
                     /translation="MAITSTLPVFEAAGAPLTQSGIGAASALLNPRVLGVMSVDARVR
                     YLQARARVSPSQDFVALLRKAMEFERAFVQAGGLLVAGLDPTGNGGVVAGFGDLRQVE
                     LLVEAGFTPLEAIKIASFNGAKFLGEDARIGSIAVGKQADLVVVKGNPAANIGDIEKV
                     EVVFKDGVGYDSEKLIQSVQGLVGIR"
     gene            complement(1572..2441)
                     /locus_tag="AC159P_12790"
     CDS             complement(1572..2441)
                     /locus_tag="AC159P_12790"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014081.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR3696370.1"
                     /translation="MKVKLPVLLLLFCLVITSFAQAPDSRQFIRVEAPTVALAHVRVI
                     DGTGAAPLEDRTIVISGGKIQSIEASAAAKAPAGAQVLDLAGYTVLPGLVGMHNHLFF
                     PMGGSPPMYSNMGGSFPRLYLALGVTTIRTTGSVAPYTDLEIKRLIDAGRMIGPKMHV
                     TAPYLEGAGAFTPVMRQLTGPDDARRMVNYWADAGATSFKAYMNITRDELRAVVEEAH
                     KRGLKVTGHLCSVGYREAAEIGIDNLEHGLFADSEFVADKKADQCPPGGAVSASLRQL
                     DLNGPAARETIRT"
BASE COUNT          462 a          863 c          856 g          426 t
ORIGIN      
        1 gccttggtga ggtttcaagc cctgcgcgta ctccgccagc ggcggattca attgcagccc
       61 gtccgggcgc gcacggtagc ggaagccgta aaacgaccac aacaccgcga ccgcgatgac
      121 gacgatggcc gccagcgcgg cggcgaattt cagcgcctgc ttacctgatt cctttgtcgc
      181 tgttgtgccg tcccctttgg cggcgcggcg gtgacgcaac acttcgcaga cggcgagcac
      241 gaacagcatg gggaacacga aaatgcccgt gtgcttgacg gcgagcgcaa gccccgccga
      301 cacgccgacg agcgccacgc gccagaccga aggggctttg gcgaaacggt agaaggcgta
      361 gatggtcgcc agcatgaagc aggaaatccc cgcgtcggtc gtgacgagcg cgccgtgggc
      421 cagcaggttc ggctcgaagg ccagcagcgc gagcgcgatg aaagccgcgc ccgtgccgaa
      481 catctccctc gtcgccacga agacgagcag cgccagcagg agcgtcagcg tggccgccgc
      541 catgcgcgtg cggaagagga tcatgtccgc gtcgttccgg tagaggaagt ccttgccgcc
      601 gaggaaggcc gcctctttga aatagcgatc ctgcggctcg ggggctttca gcggcagagg
      661 gaggagcgga gcggtggcca gcagtttcac cagcggcggg tgctccgggt taagtccgaa
      721 gtccgcgcgc cgccacgaca tgtagcccgc gtagatgtgg ttgccttcgt cccatgtctg
      781 ggattcccgt cgcaccgaga gcgccaactg aagaccgagt atcagcaaaa gggcgacgac
      841 ccccgcggcc accgccccac cccgccggct tacagagctt ccgaccatgc gccctccatc
      901 gtgagacgct tcgtgaagac ctttgcgaaa ggtctcttca acgaaagcta tcggactgtc
      961 cctccccgcc tcgaaagcta ttcgcgcaac acggccgttc aacgtatccc gaccagcccc
     1021 tgcaccgatt ggatcaactt ctccgagtcg tacccgacgc cgtccttgaa gacgacctcc
     1081 accttctcga tgtcgccgat gttcgccgcc gggtttccct tgacgaccac gaggtcggcc
     1141 tgcttgccga cggcgatgga accgatccgc gcgtcctcgc cgagaaactt cgcgccgttg
     1201 aagctggcga tcttgatcgc ctccaaaggc gtgaagcccg cctcgaccag cagctcgact
     1261 tggcgcagat cgccgaagcc ggcgacgacg ccgccgttgc ccgtcgggtc gagtccggcg
     1321 acgagcaacc ctcccgcctg cacgaacgcc cgctcgaact ccatcgcctt gcgcaacagc
     1381 gcgacgaaat cctgcgaggg cgaaacgcgc gcacgcgctt gcaggtagcg cacgcgcgca
     1441 tccacggaca tcacgccgag cacacgcggg ttcagcaacg cactcgcggc gccgatgccg
     1501 ctctgggtca acggagcgcc cgcggcctcg aacaccggaa gcgtcgaggt gatggccacg
     1561 ttcttggcaa cttaagtgcg gatcgtctcg cgcgcggcgg ggccgttcag gtcgagttgt
     1621 cgcaggctcg cgctgacggc gccgcccggc ggacactgat cggctttctt gtcggcgacg
     1681 aattcggaat cggcgaacag cccgtgctca aggttgtcga tgccgatctc cgcggcttcg
     1741 cggtagccga ccgagcagag atggcccgtg actttcaacc cgcgcttgtg cgcctcttcg
     1801 acgaccgcgc gcagctcgtc gcgcgtgatg ttcatgtacg ccttgaacga ggtcgcgccc
     1861 gcgtcggccc agtagttcac catgcgccgc gcgtcgtccg gcccggtcag ttgacgcatg
     1921 acgggcgtga acgcgcccgc gccttcgagg tagggcgccg tgacgtgcat cttcgggccg
     1981 atcatccggc ccgcgtcgat gagccgcttg atttcgagat cggtgtacgg tgcgacgctg
     2041 cccgtggtgc ggatggtggt gacgccgagc gcgaggtaca ggcgcgggaa gctgccgccc
     2101 atgttcgagt acatcggcgg ggagcctccc atcgggaaga acaggtggtt gtgcatcccg
     2161 acgaggccgg gcagaaccgt atagcccgcg aggtcgagca cctgcgcgcc cgccggagct
     2221 ttcgccgcgg cgctcgcctc gattgactga atcttgccgc cgctgatgac gattgtccga
     2281 tcctcaagcg gcgcggcgcc cgtcccgtcg atcacccgca cgtgcgcgag cgccaccgtc
     2341 ggcgcttcga cgcggatgaa ctgccggctg tcgggcgcct gcgcgaagga agtgatgacg
     2401 aggcagaaga gaaggagaag aacggggagt ttgaccttca tcgctttgca gtcctttacg
     2461 tgtgccgggg ttgtggcgcg caaggataat agtgccgcga ctgaatgaat gttaagggca
     2521 aaagtggcgg cgctcgtccc cgaagtcatc gacgggcgct cgaaggtctt gaccgccgcc
     2581 tgacgcgggg acgcgtcgcg gtagtta
//
LOCUS       DBNGOH010000656         2065 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_829193, whole genome shotgun sequence.
ACCESSION   DBNGOH010000656 DBNGOH010000000
VERSION     DBNGOH010000656.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2065)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_829193"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1841)
                     /locus_tag="AC159P_12795"
     CDS             complement(<1..1841)
                     /locus_tag="AC159P_12795"
                     /inference="COORDINATES: protein motif:HMM:NF012356.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HIR3696371.1"
                     /translation="MTARFLLPVLCTLVCLAAGARAQNHVPVVEKIEPPSWWAGHTIN
                     PVRLLVRGKSLNYVRMSAADNAPFEVGESRAKASGTYMLVDLYISPTARPGDYRLRFS
                     TGMGAAFHTFRINAPLDPGKNFQGITKDDVIYLVMPDRFADGDPANNAPRDAPPEAND
                     RRNARAWHGGDLRGIINRLPYLEELGVTALWLNPWHDNWNGVNRCDKPWCPNTYYHGY
                     HAVDYYGVEDRFGDLATLRELVDKAHAHGIKIIQDQVANHVVTRHPWAQDPPLDNWFH
                     GTVAGHTLNPFRGDLLLSPHAPDAARRPTLDGWFADDMPDMNQEEPEVARYEIQNALW
                     WVGVTGMDGIRQDTIQYMPRAFIRDLSRALHKQYPRMWMVGEVFDRDPVHTAFFMGGR
                     AGWDRIDTELDSVFDFHTWQTSLDVFTGKRPARHLRDALKYDAAYPDASRLTTMANNH
                     DVKRFMSLEGATLEGAMMHVAFTLSIRGTPQLYYGEEIAMEGGDDPDNRRDFPGGFPG
                     DPRDAFTRAGRTPREQRMFEWTRDWIRLRRERSSIRRGATLDLFFDDDAYAFARRDDK
                     ETTVIVFNRAAAPKEIPLQADLLGARDGARLEPLRGAQDRALFAAGAA"
BASE COUNT          293 a          632 c          774 g          366 t
ORIGIN      
        1 gccgcgccgg ccgcgaaaag tgcgcggtct tgcgccccgc gcaaaggttc gaggcgcgcg
       61 ccgtcgcgcg ctccgagcag gtcggcctga agcggaatct ccttgggggc ggcggcgcgg
      121 ttgaagacga tgacggtcgt ctccttgtcg tcgcggcgcg cgaaggcgta ggcgtcgtcg
      181 tcgaagaaca ggtcgagcgt cgcgccgcgg cggatggaag agcgctcgcg gcgcaggcgt
      241 atccagtcgc gtgtccactc gaacatgcgc tgctcgcgcg gcgtgcggcc cgctctggtg
      301 aaggcgtcgc gcgggtcgcc ggggaagccg cccggaaagt cgcggcggtt gtcggggtcg
      361 tcgccgccct ccatcgcgat ctcctcgccg tagtagagct gcggcgtgcc ccgaatcgag
      421 agcgtgaagg cgacgtgcat catcgcgcct tcgagcgtgg cgccttcgag cgacatgaag
      481 cgcttcacgt cgtggttgtt ggccatcgtc gtgaggcgcg aggcgtccgg gtaggcggcg
      541 tcgtacttca aggcgtcgcg caagtgccgc gcgggccgct tgccggtgaa cacgtcgaga
      601 gacgtttgcc acgtgtggaa gtcgaagacc gaatccagct cggtgtctat tcgatcccag
      661 cccgcgcgcc cgcccatgaa gaaggccgtg tggacggggt cgcggtcgaa gacctcgccg
      721 accatccaca tccgcgggta ctgcttgtgg agcgcgcggc tcaggtcgcg gatgaaggcg
      781 cggggcatgt actggatcgt gtcctggcgg atgccgtcca tgcctgtgac gccgacccac
      841 cagagcgcgt tctgtatctc gtagcgcgcc acctccggct cttcctgatt catgtcgggc
      901 atgtcgtcgg cgaaccagcc gtcgagcgtc gggcggcgcg cggcgtcggg cgcgtggggc
      961 gagagcagca ggtcgccgcg gaaggggttg agcgtatgac ctgcgacggt gccgtggaac
     1021 cagttgtcga gcggcgggtc ttgcgcccac gggtggcgcg tgacgacgtg gttggcgacc
     1081 tgatcttgaa taatcttgat gccgtgggcg tgggctttat cgaccagctc gcgtaaggtc
     1141 gcgaggtcgc cgaagcggtc ttcgacgccg tagtaatcga cggcgtggta gccgtgataa
     1201 taggtgttcg ggcaccacgg cttgtcgcag cggttgacgc cgttccagtt gtcgtgccag
     1261 gggttcagcc agagcgcggt gacgccgagt tcttcgaggt aaggcaggcg gttgatgatg
     1321 ccgcgcaagt cgccgccgtg ccacgcgcgt gcgttgcgac ggtcgttggc ttccgggggc
     1381 gcgtcgcgcg gcgcgttgtt cgcggggtcg ccgtcggcga agcggtcggg catgacgagg
     1441 tagatcacgt cgtccttcgt gatgccctga aagtttttgc cggggtcgag cggcgcgttg
     1501 atgcggaagg tgtggaaggc ggcgcccatg cccgtggaga agcgcagcct gtaatcgccc
     1561 ggcctcgccg tcgggctgat gtagaggtct acgagcatgt aggtgccgga cgccttcgcc
     1621 ctcgactcgc ccacctcgaa cggcgcgttg tccgcggcac tcatccgcac gtagttcagg
     1681 ctcttccccc gcacgagcag gcgcacgggg ttgatcgtgt gaccggccca ccacgaaggc
     1741 ggctcgatct tttcgacgac ggggacgtgg ttctgcgccc gcgcgcccgc ggcgaggcag
     1801 acgagcgtgc agagaacggg aagaaggaaa cgcgccgtca acttggtcgg ttgagtcctc
     1861 atgctatgcg ctaactgtcg gcagttccgg cgtcttcagt gtggcggttt cgcgccctcg
     1921 cccgagaccg aggcggggag cggagcgggc gcggccacgt cgccgtcacc catgccgggc
     1981 gccgcggtct cgccgccgag cggcgtcggg gcgtgtttac gcgcctcctc gaagcgcttg
     2041 aagatggcga gttcacggtc ggcgt
//
LOCUS       DBNGOH010000657         1596 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2846581, whole genome shotgun sequence.
ACCESSION   DBNGOH010000657 DBNGOH010000000
VERSION     DBNGOH010000657.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1596)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1596)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1596
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2846581"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            272..1549
                     /locus_tag="AC159P_12800"
     CDS             272..1549
                     /locus_tag="AC159P_12800"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008476250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val family dehydrogenase"
                     /protein_id="HIR3696372.1"
                     /translation="MSRQQAARAPQEELNPFLIAKQQFDAAADLLGIDESMRQVLKNP
                     KRQLIVSIPVKMDGGDVRVFEGYRVQHNIARGPAKGGIRFHPGVTLDEVKALASWMTW
                     KCATVGIPYGGGKGGVVCDPKSLSRGELERLTRRYAFEIAPIIGPDRDIPAPDVYTDE
                     QTMAWIMDTISMVRGHTELGVVTGKPLSLGGSRGRAEATARGCLYVLREACRVRGMSL
                     GGARVVVHGFGNAGANVARLVEADGARVIAIADSRGAVHNERGISVQAALRHKETTGA
                     VSGLADTTPVGRDEVLGIDCDILLPAGLENTITMGNVRGVRTKIIGELANGPVTPGAD
                     RVLFDQGVMLLPDILANAGGVTVSYYEWVQDLYSFFWSEKQVNDTLEQTMKASFDDVY
                     ATAEKYRTDMRTGAYILAIGRVSEATSVRGIFP"
BASE COUNT          278 a          527 c          545 g          246 t
ORIGIN      
        1 gtgccgtcgg gtcagggagt cactcacgtt agaccccgcg cgcgttccgc gggttccaaa
       61 aaaagaagcg ggcggcaggc agcaggcagc gggcagttaa cgacaaaaag cagttgcgat
      121 ttgaaaactc aagttcaagg ttcctgttcc ctcttcttct tgctgcctgc tgcccgctgc
      181 ttgctgcccg ctgtctcttg aactttgggc cttgacctca tacgatgtcg cacgcttcga
      241 ggcggttatc ccacttcaga ggagagtgcc gatgagcaga cagcaagcgg cccgcgcccc
      301 gcaggaagaa ctcaacccgt tcctcatcgc caagcagcag ttcgacgcgg cggcagactt
      361 gctcgggatc gacgaatcga tgcggcaggt cttgaagaac ccgaagcggc agttgatcgt
      421 ctcgatcccc gtcaagatgg acggcggcga cgtgcgcgtc ttcgagggct accgcgtcca
      481 gcacaacatc gcgcgcggcc ccgcgaaggg cggcatccgc tttcatccgg gcgtgacgct
      541 cgacgaggtg aaggcgctcg cgtcgtggat gacgtggaag tgcgccacgg tcggcatccc
      601 ctacggcggc ggcaaaggcg gcgttgtgtg cgacccgaag tctctgtcgc gcggcgagct
      661 tgagcgcctg acgcgccgct acgccttcga gatcgcgccc atcatcggcc ccgaccgcga
      721 catccccgcg ccggacgtgt acaccgacga gcagacgatg gcgtggatca tggacaccat
      781 ctcgatggtg cgcggccaca cggaactcgg cgtcgtgacg gggaagccct tgtcgctcgg
      841 cggctcgcgc gggcgcgcgg aggcgacggc gcgcgggtgc ctctacgtgc tgcgcgaggc
      901 gtgccgcgtg cgcggcatga gcctcggcgg cgcgcgcgtg gtcgtccacg gcttcggcaa
      961 cgcgggcgcc aacgtcgcgc ggctcgtgga ggcggacggc gcgcgcgtca tcgccatcgc
     1021 ggactcgcgg ggcgccgtcc acaacgagcg cggcatctcc gtccaggccg cgctcaggca
     1081 caaagagacg acgggcgccg tcagcggtct ggccgacacg acgccggtgg gccgcgacga
     1141 ggtgctcggc atagactgcg acatcctcct gcccgcgggg ctggagaaca cgatcacgat
     1201 gggcaacgtc cgcggcgtgc ggacgaagat catcggcgag ctggcgaacg gccccgtcac
     1261 gcccggcgcc gaccgcgtgc tcttcgacca gggcgtcatg ctgctgcccg acatcctcgc
     1321 caacgctggc ggcgtcaccg tcagctacta cgagtgggtg caagacctct actccttctt
     1381 ctggtccgaa aagcaggtca acgacacgct cgaacagacg atgaaggcgt ccttcgacga
     1441 cgtgtacgcg accgccgaga agtaccggac cgacatgcgc accggcgcct acatcctcgc
     1501 catcggtcgt gtctccgaag cgaccagcgt gcgcggcatc tttccgtaaa aagaggggtc
     1561 gggggccggg ggccagtcaa aatcgggggg gggggg
//
LOCUS       DBNGOH010000658         2522 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4678519, whole genome shotgun sequence.
ACCESSION   DBNGOH010000658 DBNGOH010000000
VERSION     DBNGOH010000658.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2522)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2522)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2522
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4678519"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..946)
                     /gene="glyA"
                     /locus_tag="AC159P_12805"
     CDS             complement(<1..946)
                     /gene="glyA"
                     /locus_tag="AC159P_12805"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006445907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0006545 - glycine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HIR3696373.1"
                     /translation="MDDRKNATLADVDPLISRAIDDEVRRQAEGLELIASENFVSEAV
                     LEAMGSVFTNKYAEGYPGKRYYGGCEFTDVVEQAAIDRAKDIFGAEHANVQPHSGSQA
                     NMAVYLAALQYGDTILGMNLSHGGHLTHGHPLNFSGFSYKVADYGVSRETETIDYDEL
                     ERIAKESKPKMIVCGASAYPRTIDFERIGAIAKAVGARMMADIAHIAGPIAAGLHPSP
                     IPHADYVTTTTHKTLRGPRGGLVLCRAEHAAEIDRKLFPGIQGGPLVHIIAAKAVAFG
                     EALREDFREYQRRILSNAKALADGLQEQGFRLVSGGTDN"
     gene            1293..2402
                     /locus_tag="AC159P_12810"
     CDS             1293..2402
                     /locus_tag="AC159P_12810"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015531.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HIR3696374.1"
                     /translation="MRFAKAFMMLALFAAAIIVDPAQGLGQMQAPEQPDLTIDAATRK
                     EVIDTLLKRLNESYVFPETAAKMEQAVRARLEKKEYDQITSAKEFARKLTEDVQAVSR
                     DKHLRVRYSHQPIPERGQRREPSAEDRERFQREMSRINFGFEKVERLPGNIGYVDFRG
                     FFAPDLGKETVASVFNFLANTDALIIDLRKNGGGDPHMVALISSYLFGPEPVHLNSLY
                     WREGNRTDDFWTAKEVAGKRYNGKDVYVLTSNRTFSGAEEFSYNLKNLKRATIVGETT
                     GGGAHPGGGNRLHANFGAFIPTGRAINPITKTNWEGTGVEPDVKVPADQALKVAHLSA
                     LKKAYEKTTDKEMKDALQRLIDSMQKEVSEVPAKK"
BASE COUNT          456 a          839 c          792 g          435 t
ORIGIN      
        1 ggttgtccgt gccgcccgag acgaggcgga agccctgctc ttgcagaccg tcggcgagcg
       61 ccttcgcgtt cgagaggatg cggcgctggt attcacggaa gtcttcgcgc agagcctcgc
      121 cgaaggcgac cgccttcgcc gcgatgatgt ggacgagcgg cccgccctgt atgcccggaa
      181 agagtttccg atcgatttcc gccgcgtgct ctgcgcggca gaggacgagg ccgccgcgcg
      241 ggccgcgcaa ggtcttgtgc gtggtggtgg tcacgtagtc tgcgtgcgga atgggcgaag
      301 ggtgcagccc cgccgcgatg gggccggcga tgtgcgcgat gtcggccatc atccgcgcgc
      361 cgaccgcctt ggcgatggcg ccgatgcgct cgaagtcgat cgtgcgcggg taggccgacg
      421 cgccgcagac gatcatcttc ggcttgctct ctttggcgat gcgctcaagc tcgtcgtagt
      481 cgatcgtctc ggtctcgcgc gagacgccgt agtccgcgac cttgtagctg aagccggaga
      541 agttcagagg gtggccgtgc gtgaggtggc cgccgtgcga gaggttcatg ccgaggatcg
      601 tgtcgccgta ctgaagggcc gcgaggtaga ccgccatgtt ggcctgcgag ccggagtgcg
      661 gctgcacgtt ggcgtgctcg gcgccgaaga tatccttcgc gcggtcgatg gccgcctgct
      721 cgaccacgtc cgtgaactcg cacccgccgt agtagcgctt gccggggtag ccttccgcgt
      781 acttgttcgt gaagaccgac cccatcgctt cgagcacggc ctcgctcacg aaattctccg
      841 aggcgatcag ctccagcccc tccgcctgcc gccgcacctc gtcgtcgatc gcgcggctaa
      901 tcaacgggtc aacatcggcg agcgtcgcgt tcttgcggtc gtccatcggc ggtctccttc
      961 tcgcgggcct gggttttcgt agcggcgttc ggattaacga gtgcctgatg ttacctcacg
     1021 actcccgcgc ccgcaacgcc cttaagagcc gtgaaccgta aaacgtgaac cgtgacaaga
     1081 aaagaccgat tctttcttgt cacggttcac ggtttacggt tcatggctct tcctcggtac
     1141 ttttatttcg aagattttgg aactcggctt gctttttgac gtaattatga ccagaatgcg
     1201 ttttcggtca gcagtcagtg cgtggccgca gactcacgag cagcaagcag gggcgcccat
     1261 agcccgccaa ccacttcaag gagattcgag agatgagatt cgccaaagct ttcatgatgc
     1321 tcgccctctt cgccgccgcg atcatcgttg acccggcgca gggcctcggc cagatgcagg
     1381 cgcccgagca gcccgacctg accatcgacg ccgccacgcg caaggaggtg atcgacaccc
     1441 tcctgaagcg cctcaacgag tcgtacgtct tccccgagac cgccgccaag atggagcagg
     1501 cggtgcgcgc gcgtcttgag aagaaggagt acgaccagat cacgagcgcc aaggagttcg
     1561 cgcgcaagct gaccgaggac gtgcaggccg taagccgcga caagcacctg cgcgtgcgct
     1621 actcgcacca gccgatcccc gagcgcggcc agcggcgcga acccagcgcc gaagaccgcg
     1681 agcggttcca acgcgagatg agccgcatca acttcggctt cgagaaggtc gagcgcctgc
     1741 ccggcaacat cggctacgtg gacttccgcg gcttcttcgc acccgacctc ggcaaggaga
     1801 ccgtggcctc ggtcttcaac ttcctcgcca acaccgacgc gctcatcatc gacctcagga
     1861 agaacggcgg cggcgacccg cacatggtcg cgctcatctc ctcgtacctc ttcggccccg
     1921 aacccgtcca cctcaactcg ctctactggc gcgagggcaa ccgcaccgac gacttctgga
     1981 cggcaaagga ggtcgcgggc aagcgctaca acggcaagga cgtgtacgtg ctcaccagca
     2041 accgcacctt ctcgggcgca gaggagttct cctacaacct caagaacctg aagcgcgcga
     2101 ccatcgtcgg cgagaccacg ggcggcggcg cgcaccccgg cggcggcaac cgcctgcacg
     2161 ccaacttcgg cgccttcatc cccacgggcc gcgccatcaa ccccatcacc aagaccaact
     2221 gggaaggcac gggcgtcgag ccggacgtga aggtccccgc cgaccaggcg ctcaaggtcg
     2281 cgcacctctc ggcgctcaag aaggcttatg agaagacgac cgacaaggag atgaaggatg
     2341 cgctccagcg tctgatcgac agcatgcaga aggaagtgag cgaggttccg gccaagaagt
     2401 agctcaagct cacacgcgag ggccgtaggg gcgcgggcac gaggtgccgc gccccttcgc
     2461 ttttctccgt ggttcaagcc gtggttgctt tcccctctcc cactgcgcga gaatggccgt
     2521 tc
//
LOCUS       DBNGOH010000659         3351 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2663664, whole genome shotgun sequence.
ACCESSION   DBNGOH010000659 DBNGOH010000000
VERSION     DBNGOH010000659.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3351)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3351)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3351
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2663664"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(3..1559)
                     /locus_tag="AC159P_12815"
     CDS             complement(3..1559)
                     /locus_tag="AC159P_12815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HIR3696375.1"
                     /translation="MKRSILLMLHAAALTLALCGAAQEAAGKTVHVHLLDGDDARGDG
                     SYAKPYKSWRKALQQAGSGDTVVAKNGDYRKAGQAARWGGIHLVLTMADKLDPGDPRQ
                     PVPDPARPETVGVYRYDPARPLTIRAETRHGVIIDSVRLHLVRGIVIDGFDINPNPYY
                     ADAAGKKINRRRNGVHGDSVYEPEDRFNKVKTNDPPGSYTAAWYDRSLWTSHVTVSNC
                     KVHYECPPAGCTPEYDPLQDDDRLYLVKFNQSHHITIEDNELFDGRNAQRKPAIDLPC
                     VDDAVVRRNLIRNSHRGVVSKGGGRRVLIEANVFVDNSGAAFSGGSTDPDLFIDGRVG
                     DPCSFARFESYEMTARDNLVVSTRPGERPVEPVSLWAAKDAVVVNNTFVGVGERGVLL
                     VRPGNEVDSPARGCGRSVRLPLTEGLTFRNNLFVLSGVVDETMLYQTSGEGVKVAGFV
                     SDHNTFYNGGRDVPVGGFADPNREPGFSKADPLLAGGRGTDYASWMATAKLGKGSPSR
                     GRGVRDASGR"
     gene            complement(1571..2908)
                     /locus_tag="AC159P_12820"
     CDS             complement(1571..2908)
                     /locus_tag="AC159P_12820"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019940860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR3696376.1"
                     /translation="MKVFFGLFSTALVLTAMCVPASAQRRAGGGERRATPARISEVNQ
                     IKGADAGRTFAIVGATLIDGRGGSPVADSVVVVRGERIVAAGARASVRVPVGAEVFEA
                     RGLTLLPGLIDAHFHIDGDDGLPALFLSHGVTSLRDPGQWIEAYDAARAAGPSVPRLF
                     LTGPHLDSPPAAYPADSFVVRDAEEARLAVKRFAEQGASAFKVYFRLPVGLIRVVCEE
                     AHRRGLPVTSHLEIVDAADAVRAGVDGVEHVTSFGTAMLPARDAEKYRQAVLADNAAR
                     REGRYEVWSKADLDGPRARAVISLLAERGTYLSPTLAIFERRAGDKDTKDIHVGGFKK
                     MLEFTGRARRAGVRVVVGSHSSVPHAERGWAYQRELELLVEAGMTPMQAIVAGTSENA
                     RFFRVEERLGSVEPGKLADLLLVEGEPLKDIRVMRNVRRVMLGGAWVAAPQGK"
     gene            complement(2957..>3351)
                     /locus_tag="AC159P_12825"
     CDS             complement(2957..>3351)
                     /locus_tag="AC159P_12825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696377.1"
                     /translation="EDAFLNVLEIGERGRMAVRKVELADGANLTGAAIEGGVLALFAA
                     ADARVTEGEATVPDVETTALLVTGLVPDAKYELQLTGGRANWRGGLFNGVPQWQQVAT
                     ADGSGVIYLPTFKGQRDGRLRVRLLSRE"
BASE COUNT          482 a         1152 c         1183 g          534 t
ORIGIN      
        1 ttctatctcc cggaggcgtc gcgcacgccc ctgccccgcg agggcgaacc cttcccgagc
       61 ttcgcggtcg ccatccatga ggcgtagtcg gtcccgcgcc cgccggccag cagcgggtcg
      121 gctttgctga agcccggctc gcggttcggg tcggcgaagc cgccgacagg cacgtcgcgc
      181 ccgccgttgt aaaaagtgtt gtggtcgctg acgaagcccg cgactttcac gccctcgccc
      241 gaggtctggt agagcatcgt ctcgtccacg acgcccgaga ggacgaagag gttgtttctg
      301 aacgtaagcc cctctgtgag cgggaggcgc acgctccgcc cgcaaccgcg cgcggggctg
      361 tccacctcgt tgccggggcg gacgagcagc acgccgcgct cgccgacgcc gacgaaggtg
      421 ttgttgacga ccacggcgtc cttcgccgcc cagagcgaga caggctcgac ggggcgctcg
      481 cccggtcggg tcgagacgac gaggttgtcg cgcgccgtca tctcgtacga ctcgaagcgc
      541 gcgaacgaac aggggtcgcc cacacgcccg tcgatgaaga ggtcggggtc ggtcgagccg
      601 cccgagaagg ccgcgcccga gttgtccacg aagacgttgg cctcgatgag cacgcggcgg
      661 cctccgccct tcgacaccac gccgcggtgg ctgttgcgga tcaggttgcg ccgcacgacg
      721 gcgtcgtcca cgcagggcag gtctatggcc ggcttgcgct gcgcgttgcg accgtcgaag
      781 agttcgttgt cctcgatggt gatgtggtgc gactggttga acttgacgag gtagagccgg
      841 tcgtcgtcct gcaaagggtc gtactcgggc gtgcacccgg caggcgggca ctcgtagtga
      901 accttgcagt tgctgacggt gacgtggctc gtccacagag agcggtcgta ccacgcggcg
      961 gtgtagctgc ccggcgggtc gttcgtcttc accttgttga accggtcttc cggctcgtag
     1021 acgctgtcgc cgtggacgcc gttgcggcgg cggttgattt tcttccccgc cgcgtcggcg
     1081 tagtagggat tcgggttgat gtcgaagccg tcgatcacga tcccgcggac gaggtggagg
     1141 cgcaccgagt cgatgatgac gccgtgcctg gtctcggcgc ggatggtgag cgggcgcgcg
     1201 gggtcgtagc ggtagacgcc gacggtctcg gggcgcgcgg ggtctgggac gggctggcgc
     1261 gggtcgccgg gatcgagttt gtcggccatc gtcaggacga ggtggatgcc gccccagcgc
     1321 gcggcctgac ccgccttgcg gtagtcgccg ttcttggcga cgacggtgtc gccgctcccc
     1381 gcctgttgca gcgccttgcg ccaggacttg taaggtttcg cgtagctgcc gtcgccgcgc
     1441 gcgtcgtcgc cgtcgagcag gtggacgtgg acggtcttgc ccgcggcctc ctgcgcggcc
     1501 ccgcacaacg cgagcgtcag ggctgccgcg tgtagcatga gaaggatgct tcgcttcaac
     1561 tctcccctcc tcacttgccc tgcggcgcgg cgacccacgc gccgccgagc atgacgcgcc
     1621 tgacgttgcg catgaccctg atgtctttga gcggttcgcc ttcgacgagc aagaggtcgg
     1681 cgagtttccc cggctcgacg ctccccagcc gctcttcgac gcggaagaag cgcgcgttct
     1741 ccgaggtgcc ggccacgatg gcctgcatcg gggtcattcc cgcctcgacc agcagctcaa
     1801 gctcgcgctg gtaggcccag ccgcgctcgg cgtgcggcac tgacgagtgc gagccgacga
     1861 cgacgcggac gcccgcgcgc cgcgcgcgcc cggtgaattc gagcatcttc ttgaagccgc
     1921 cgacgtgaat gtccttcgtg tccttgtcgc ccgcgcgccg ctcgaagatg gcgagcgtcg
     1981 gcgacagata cgtgccgcgc tcggccagca gggagatgac ggcgcgcgcg cgcgggccgt
     2041 cgaggtcggc cttgctccag acctcgtagc gcccctcgcg ccgcgcggcg ttgtcggcga
     2101 gcacggcctg tctgtacttc tccgcgtcgc gcgcagggag catcgccgtg ccgaaggagg
     2161 tgacgtgctc gacgccgtcc acgcccgcgc ggacggcgtc ggctgcgtca acgatctcca
     2221 agtgcgaggt gacgggcagc ccccgccggt gtgcctcttc gcagacgacg cggatgagac
     2281 cgacggggag gcggaagtag accttgaagg cggaggctcc ctgttcggca aaacgtttga
     2341 cggcgagtcg cgcctcctcc gcgtcgcgga cgacgaaaga gtctgccggg taggccgcgg
     2401 gcggcgagtc gaggtgcggc cccgtcagga agaggcgcgg gacggacggg ccggcggcgc
     2461 gcgcggcatc gtaagcctcg atccactggc cggggtcgcg caagctcgtg acgccatgcg
     2521 agaggaaaag cgcgggcagc ccgtcgtcgc cgtcgatatg aaagtgcgca tcgatgaggc
     2581 cgggcagcag cgtcaacccg cgcgcctcga agacctcggc acccaccggg actcgcacgg
     2641 aagcgcgcgc gcccgcggcg acgatgcgct cgccgcgcac gacgaccacg gagtcggcga
     2701 cgggcgaacc gccgcgcccg tcgatcagcg tcgcgccgac gatggcgaat gtgcgccccg
     2761 cgtcggcgcc tttgatttgg ttgacctcac tgatccgtgc aggcgtcgcg cgcctttcgc
     2821 cgccgcccgc gcgacgctgc gcggatgcgg ggacgcacat ggcggtcaga accagcgccg
     2881 tcgaaaagag tccaaagaaa actttcatca cgatctgctt ccgagggcgc ggcctgatgc
     2941 cgcgccgtgg tggtcgctac tcccttgaga gcagacggac gcgcaggcgt ccgtcccgct
     3001 gacccttgaa cgtgggcagg tagataacac ccgagccgtc ggcggtcgca acctgctgcc
     3061 actgcggcac gccgttgaag agtccgccgc gccagttggc ccggcccccc gtcaactgca
     3121 actcgtactt cgcgtcgggg acaagccccg tgacgaggag cgccgtcgtc tcaacgtcgg
     3181 gcacggttgc ctcgccctcc gtaacgcgcg cgtccgccgc cgcgaacagc gccagcacgc
     3241 cgccctcgat ggccgcgccc gtcaggttcg cgccgtcggc cagctcgacc tttcgcacag
     3301 ccatccggcc gcgttcgccg atctccagaa cgttgaggaa tgcgtcctcc t
//
LOCUS       DBNGOH010000660         2423 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3213697, whole genome shotgun sequence.
ACCESSION   DBNGOH010000660 DBNGOH010000000
VERSION     DBNGOH010000660.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2423)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2423)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2423
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3213697"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1978)
                     /locus_tag="AC159P_12830"
     CDS             complement(<1..1978)
                     /locus_tag="AC159P_12830"
                     /inference="COORDINATES: protein motif:HMM:NF013100.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HIR3696378.1"
                     /translation="MVAVFLCAWMVVVGARLAYLQTGDRHEQLAQRARAQQTDAETLS
                     PVRGLILDRQGRELARSVDADSFFAVPGEIEDFDAAAQSLARVVGTDAPSLAARLREA
                     KEAKRSFVWVARKVTEEEGAAVRELKLKGVHSLQEPRRRYPNGDLAAHVLGHVGMDDK
                     GLAGVEQFHNAALTGEAGRVVLEGDARRRMYESFGVDPRPGQTVVLTIDQVVQFRAEQ
                     AIKAAVERSRAKSGTAIVLDPRTGEILALANAPAFDPNHVGKDGELARANDALQNIYE
                     PGSTFKIVAHSAALEEGLARPDEKIDCQMGGITIAGRRIRDHTAYGTLTMTEAMAKSS
                     NVAAIKLGMRVGDPRMYDYIRRYGFGTRTGVELPGETAGLLRPVERWQKSSIGSIAIG
                     QEIGVTPLQMAAAFGALANDGVRIAPHLVREVRDAEGKTVRRTEAESRRVVSAETARA
                     VRMMLETVTLKGTAKRAQLEGYTAAGKTGTAQKIDPKTRAYSKTKHVASFAGFAPVEN
                     PSVVIVVVVDEPVGAYHGGDVAAPIFREIADQILPFLGVTPDEEPEAPAVEEEERLLA
                     QAARAAEEQRRATEAEVRVLLPEVVRGEVGEVVYAAAAERALLMPDLRGRSVRDAARV
                     CAQLGLELEARGEGRAVSQSPAAGASVGAGEVVRI"
     gene            complement(2198..>2423)
                     /locus_tag="AC159P_12835"
     CDS             complement(2198..>2423)
                     /locus_tag="AC159P_12835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696379.1"
                     /translation="EQVLDEQRRLLYALEEHSSPANLERAAREMNLELQPARSAQIDA
                     GAREREPRGEAPSARRAAVGAGVGGAVLRR"
BASE COUNT          317 a          941 c          783 g          382 t
ORIGIN      
        1 cgatccgcac gacctcgccc gcgccgacgc tcgcgcccgc ggcggggctt tgactgacgg
       61 cgcggccctc gccgcgcgct tcgagttcca gcccgagctg cgcgcagacg cgcgccgcgt
      121 cgcgcacgct tcgaccgcgc aggtcgggca tcagcagcgc ccgctccgcc gccgccgcgt
      181 agacgacctc gccgacttcg ccgcgcacga cctcgggcaa caggacgcgc acttccgctt
      241 cggtcgcgcg cctctgctct tcggcggcgc gcgcggcctg cgcgagcagg cgctcttcct
      301 cttcgaccgc gggcgcctca ggctcttcgt ccggcgtgac gccgaggaac gggagaatct
      361 ggtcggcgat ctcgcggaag atgggcgcgg ccacgtcgcc gccgtggtag gcgccgacgg
      421 gttcgtccac gacgacgacg atcacgaccg aagggttctc gaccggcgcg aagcccgcga
      481 acgaggcgac gtgcttcgtc ttcgagtagg cgcgcgtctt cgggtcgatc ttctgcgcgg
      541 tgccggtctt gcccgccgcc gtgtagcctt cgagctgcgc gcgcttcgcc gtgcccttca
      601 gcgtcaccgt ctccagcatc atccgcaccg cgcgcgccgt ctcggcgctc acgacgcggc
      661 gcgactcggc ctccgtccgg cgcaccgtct tgccctcggc gtcgcgcacc tcgcgcacga
      721 ggtgcggcgc gatgcgcacg ccgtcgttcg ccagcgcgcc gaaggccgcc gccatctgaa
      781 gcggcgtgac gccgatctcc tgcccgatgg cgatggagcc gatggagctt ttctgccagc
      841 gctcgacggg ccggagcagc ccggcggtct cgcccggcag ctcgacgccc gtgcgcgtgc
      901 cgaagccgta gcgacggatg tagtcgtaca tcctcgggtc gccgactctc atgccgagct
      961 tgatggccgc cacgttcgaa gacttcgcca tcgcctccgt catcgtcagc gtgccgtagg
     1021 ccgtgtggtc gcggatgcgg cgccccgcga tggtgatgcc gcccatctgg cagtcgatct
     1081 tctcgtccgg cctcgccagc ccctcttcga gcgccgccga gtgcgcgacg atcttgaagg
     1141 tcgagccggg ttcgtagatg ttctggagcg cgtcgttggc gcgagccagc tcgccgtctt
     1201 tgccgacgtg gttcgggtcg aaggccggcg cgttggcgag cgccagaatc tcgcccgtcc
     1261 gcgggtcgag gacgatggcc gtgcccgact tcgcgcgcga gcgctcgacc gccgccttga
     1321 tcgcctgctc ggcgcgaaac tgcacgacct gatctatcgt cagcacgacg gtctggccgg
     1381 gccgcggatc gacgccgaaa ctctcgtaca tcctgcgccg cgcgtcgcct tcgaggacga
     1441 cgcgaccggc ctcgcccgtc agcgccgcgt tgtgaaactg ctcgaccccg gccagcccct
     1501 tgtcgtccat cccgacgtgg ccgagcacgt gcgccgccaa gtcgccgttc gggtaacgcc
     1561 gcctcggctc ctgcaaggag tgcacgccct tgagcttcaa ctccctgacc gccgcgccct
     1621 cctcttccgt caccttgcgc gcgacccaca cgaaactgcg cttcgcctcc ttcgcctcgc
     1681 gcagccgcgc cgcgagcgac ggcgcgtccg tcccgaccac gcgcgccagg ctttgcgccg
     1741 ccgcgtcgaa gtcttcgatc tcgccgggca ccgcgaagaa cgagtcggcg tccacgctgc
     1801 gcgccagctc gcggccctgc cggtcgagga tcagcccgcg cacggggctt aaggtctcgg
     1861 cgtccgtctg ctgcgcgcgc gcccgctgcg caagctgttc gtgtctgtcg cccgtctgca
     1921 agtacgcgag ccgcgcgccc accacgacca tccacgcgca aagaaaaacg gcgaccacta
     1981 aagcacgccg ccgcgagaca tcgggacgca ttgtcgctct tacagcgtat cgttcaggca
     2041 tggacggcag cggccctctc gtcatactca tcgggcttct cgcgctcctt ccgacgcgcg
     2101 ccccgttttc aagaccaccg attaaaactc gcgcgcccgg cgcgtaaggg agagacggcg
     2161 ggcgcgcgtt ttactgcact cggacacctg ataaaaacta acgccgcagc acggccccgc
     2221 ccacgcccgc gcccaccgcg gcgcgccgcg ccgacggcgc ctcgccgcgc ggctcacgct
     2281 cgcgcgcgcc cgcgtcgatc tgcgccgagc gcgcgggctg caactccaga ttcatctcgc
     2341 gcgccgcgcg ctcaaggttc gccggcgacg agtgctcttc gagcgcgtag agcaggcgcc
     2401 gctgctcgtc gagcacctgc tcg
//
LOCUS       DBNGOH010000661         5464 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_280899, whole genome shotgun sequence.
ACCESSION   DBNGOH010000661 DBNGOH010000000
VERSION     DBNGOH010000661.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5464)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5464)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5464
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_280899"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..370)
                     /locus_tag="AC159P_12840"
     CDS             complement(<1..370)
                     /locus_tag="AC159P_12840"
                     /EC_number="5.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047661 - amino-acid racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HIR3696380.1"
                     /translation="MTRHIGIVAGSAEGAALCYRTICMEAPAIMGEHNHPEITMNSMP
                     MAEHMRHIRANDWEAVAEALAASARKVAQAGADFAICPDNTYHQAFKYLLPRSPIPWI
                     HIAEAVAEEAHRLGYARLGIL"
     gene            complement(536..2338)
                     /locus_tag="AC159P_12845"
     CDS             complement(536..2338)
                     /locus_tag="AC159P_12845"
                     /inference="COORDINATES: protein motif:HMM:NF014822.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HIR3696381.1"
                     /translation="MNLNERIGAWLTAHGCFFRVWAPHAKKVSVLFQPGPYWEIDDTV
                     IEHDLVREGDYWSANVPGVPAGQLYRYRIELADGGFVERLDPAARDVLSSELTRQDPS
                     SRNGSVVPGNDAFTWAPFQTPRFENFIIYQFHIGSFAGRGDHLDKPWASFEDVVSKFA
                     YIREMGFNCIQPLPVHEFAMDRSWGYNPASFFAPESSYGSPSNLMQLVDAAHRHGLAV
                     IFDVVYNHAGPGDNVLWEYDGHANEGGIYFEGGRMTDWGRGPAWWKREVRDFFYQNAR
                     MFFEEYHADGLRFDVTTQIDGEQLKLVIDRLRRDFPDKYLTAEHLPDHPWIIDTGRFC
                     AAWHADSHHECQRALAGQDPLNKVKSFLGWDGYEHSWNLVKYTLGSHDDVGDQENGDA
                     EHGLTDWDKRHRYFIDQLGGRMNWAARAKCRLAWALNVAMPGTPLLFMGSECHMAAPH
                     VAGGYWHDGGDDGGDHRFDWGIAGDPSGMEMRRLVAACNALRWQNPALRAESLSVTHE
                     DQANQVLGFLRRLDDNVVLAVVNLGERNFTDHGYGVRTGGHGGQWTQILCTQDAAFGG
                     WDGAGNAFHDPWTQGDGRLYINLPKWSVVMFRRR"
     gene            complement(2389..3246)
                     /locus_tag="AC159P_12850"
     CDS             complement(2389..3246)
                     /locus_tag="AC159P_12850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017533285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STM4011 family radical SAM protein"
                     /protein_id="HIR3696382.1"
                     /translation="MRLSILYRGPLASCNYGCAYCPFAKHAETAAERAEDASALERFV
                     GWVAARRGDRLSVLFTPWGEALTRRRYQRALVRLTGMSHVEKAAIQTNLSCRLDWVED
                     CDKSKLGLWTTYHPSEVARGRFLSKCFELSRRGVRYSVGVVGLKEHAGEIEALRRELP
                     PDVYLWINAFKRVAGYYGSEDLERFEAVDPLFHINNRHHPSRGRACLCGESVISVAGD
                     GTVRRCHFIREPIGNIYEQGFEESLRARACTDSTCGCHIGYVHMPGLDLYSFFGGGVL
                     ERAPDNFVR"
     gene            complement(3264..4583)
                     /locus_tag="AC159P_12855"
     CDS             complement(3264..4583)
                     /locus_tag="AC159P_12855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002635052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STM4012 family radical SAM protein"
                     /protein_id="HIR3696383.1"
                     /translation="MTDLERKLRRAPYESYAYSYPHKSAYRVLEPAVGLEDAWAAEPK
                     DSLFLYLHVPFCEMRCGFCNLFTTANPEEEARDAYLKAFARQAEAVGRILCGARFARM
                     AVGGGTPTYLDPASLDSLFRIAARAFGVDARDIPASCETSPLTAERDRLRVLRERGVG
                     RLSIGVQSFDEREARAAGRPQRTEDVLAALALIREAGFPMLNIDLIYGLPGQSVRSWL
                     ASLVAALRFAPEELYLYPLYVRPLTGLERRPLEWDDIRLACYREGRALLLAEGYEQVS
                     MRMFRAARAPSDEGPAYCCQDDGMVGIGCGARSYTSALHYSNEYAVGARGVREIIARY
                     AACPPEEFERAAYGFALDGAEQRRRYLVKSLLRAEGLDAGAYRERFGTEPAEDVPELC
                     ALVESGLAEACASGPRLTNAGLELSDAVGPWLYSSKVRELMRTYELH"
     gene            complement(4580..5392)
                     /locus_tag="AC159P_12860"
     CDS             complement(4580..5392)
                     /locus_tag="AC159P_12860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014396979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STM4013/SEN3800 family hydrolase"
                     /protein_id="HIR3696384.1"
                     /translation="MLDARSLIGAHDALFITLDTLRYDVARDALAAGSTPNLARALPP
                     GGWEERHTPASFTYAAHHSFFAGFLPTPTGPGPHPRLFAARFEGSETTSERTCVFDAP
                     DIVSGLAARGYHTACVGGVGFFNKQNPLGRVLPGFFDESHWEPEFGVAEPRSTEHQIR
                     RAVETLARVPRGRRVFLFLNVSAVHQPNRFYLPGAERDSLASHAAALAYVDGQLPPLF
                     EALARRGAALVVMCSDHGTAYGEGGYYGHRVAHGVVWTVPYAEFVLPPEVCA"
BASE COUNT          881 a         1839 c         1880 g          864 t
ORIGIN      
        1 ccaggatgcc gagccgcgcg tagcccagac ggtgcgcctc ttcggcgacc gcctcggcga
       61 tgtgtatcca cgggatgggc gagcgcggga gcaggtactt aaaagcctgg tggtatgtgt
      121 tgtcggggca gatggcgaag tcggccccgg cctgcgcgac cttacgcgcc gaagccgcca
      181 gagcctcggc caccgcctcc cagtcgttcg cacggatgtg gcgcatgtgt tcggccatcg
      241 gcatcgagtt catggttatc tcggggtggt tgtgctcgcc catgatggcg ggcgcctcca
      301 tacagatcgt gcgataacac agggcggcgc cctcggcgct gccggccacg ataccgatgt
      361 gtcgcgtcat gatctcttcg cctccgccgc tccgtcggct cttcggaaat accaggggga
      421 aacgctcagg cccgccgggc cggggcaagc ccgccggatg acgccaaaga tacccacggc
      481 ggcggccccg acgcaaccgc cggttcggcg ccgccgcgta gccgaacggc atcgctcacc
      541 ttcggcggaa catgacgacg ctccacttcg ggaggttgat gtagaggcgc ccgtcgccct
      601 gcgtccacgg atcgtgaaag gcgttgccgg cgccgtccca gccgccgaag gccgcgtcct
      661 gcgtacacag aatctgcgtc cactggccgc cgtggccgcc cgtccggacg ccgtagccgt
      721 gatcggtgaa attccgctcg ccgaggttca cgacggccag caccacgttg tcgtcgagcc
      781 tgcggaggaa gccgagcacc tgattcgcct gatcctcgtg ggtgacgctc agagattcgg
      841 ccctcagcgc ggggttctgc cagcggagcg cgttgcacgc ggcgacgagc cggcgcatct
      901 ccatgcccga ggggtctccg gcgatgcccc agtcgaagcg atgatccccg ccatcgtcgc
      961 cgccgtcgtg ccagtagccc ccggcgacgt gcggcgcggc catgtgacac tccgacccca
     1021 tgaacagcag cggcgtgccg ggcatcgcta cattgagcgc ccacgccagc cggcacttgg
     1081 cccgcgcggc ccagttcatc cgaccgccga gctggtcgat gaagtagcgg tggcgcttgt
     1141 cccagtcggt cagcccgtgc tccgcgtcgc cgttctcctg atcgcccacg tcgtcgtgcg
     1201 agccgagcgt gtacttcaca aggttccagc tatgctcgta gccgtcccag ccgagaaagc
     1261 tctttacttt attcagcggg tcctggccgg cgagcgcccg ctggcattcg tgatgcgagt
     1321 cggcgtgcca cgcggcgcag aagcggcccg tgtcgatgat ccaggggtgg tcgggcaggt
     1381 gttcggcggt gaggtacttg tccggaaagt cgcgccgcag ccggtcgatg acgagcttca
     1441 gttgctcgcc gtcgatctgc gtcgtcacat cgaaccgcag cccgtccgcg tggtattcct
     1501 cgaagaacat gcgtgcgttc tggtagaaga agtcccgcac ttcccgcttc caccaggcgg
     1561 ggccgcggcc ccagtcggtc atccggcccc cttcgaagta gatgccgccc tcgttcgcgt
     1621 ggccgtcata ctcccagagc acgttgtcgc cggggccggc gtggttgtac accacgtcga
     1681 agatgaccgc gaggccgtgg cggtgggcgg catcgacgag ctgcatcagg ttggagggcg
     1741 agccgtagga cgactcgggc gcgaagaacg aggcggggtt gtagccccac gagcgatcca
     1801 tcgcgaactc gtgaaccggg agcggctgga tgcagttgaa gcccatctca cggatgtacg
     1861 cgaacttgct cacgacatcc tcgaaggacg cccagggctt gtcgagatgg tcgccgcgtc
     1921 ccgcgaagct gccgatgtgg aactggtaga tgatgaagtt ctcgaaccgg ggggtctgga
     1981 aaggcgccca agtgaacgcg tcgttgccgg gcacgaccga gccgttgcgg ctgctcgggt
     2041 cctgccgcgt cagctcggag ctgagaacgt cccgcgccgc cgggtcgaga cgctcgacga
     2101 agccgccgtc agccagttcg atccggtagc gatagagttg gcctgccggt acgccgggga
     2161 cgttcgcgct ccagtaatcc ccttcccgca cgaggtcgtg ttcgatcacc gtgtcgtcga
     2221 tctcccagta cggccccggc tggaacaaca ccgagacctt cttcgcgtgc ggcgcccaga
     2281 cgcggaagaa gcagccgtgc gcggtgagcc aggcgcctat tcgttcgttc aggttcatgt
     2341 ggggtcacgc ctcctagggc tgttccgaaa tcgacttcgg ccagacattc atctcacgaa
     2401 attatcgggc gcgcgctcca acaccccgcc gccgaagaaa ctgtagaggt cgaggccggg
     2461 catgtgcacg tagccgatgt ggcagccgca ggtgctgtcg gtgcaggcgc gcgcgcgcaa
     2521 agattcttcg aacccctgtt cgtagatgtt gccgattggc tcgcggatga agtggcagcg
     2581 gcgcaccgtc ccgtcgcccg cgacggagat gacggactcg ccgcacaggc acgcgcgccc
     2641 gcggctcggg tggtgccggt tgttgatgtg aaagagcgga tcgacggcct cgaaacgttc
     2701 gaggtcttcg ctgccgtagt agccggcgac gcgcttgaag gcgttgatcc acaggtagac
     2761 atcgggcggg agttcacgcc gcagggcttc gatctccccg gcgtgctcct tgagtccgac
     2821 gacgccgacg ctgtaacgaa ctccccgccg cgacaactcg aaacacttcg agaggaagcg
     2881 accgcgcgcc acttccgaag ggtgatacgt cgtccacagg ccgagcttgc tcttgtcgca
     2941 atcttccacc cagtcgagcc ggcaggagag gttcgtctgg atggccgcct tctcgacgtg
     3001 cgacatgccc gtgagccgga cgagcgcgcg ctggtagcgg cgtcgcgtca gcgcctcgcc
     3061 ccacggcgtg aagagcaccg agagacggtc gccgcgccgc gccgcgaccc agccgacgaa
     3121 gcgttccagc gcggacgcgt cttccgcgcg ctcggcggcg gtctcggcgt gtttggcgaa
     3181 agggcagtag gcgcagccat agttacagct cgccagcggg ccgcgataga ggatggacag
     3241 tctcacgttc gtcgtccccc gtctcagtgc agctcgtaag tccgcatcag ctcgcggact
     3301 ttcgatgagt aaagccacgg cccgacggcg tcggacaatt caagaccggc gtttgtcagc
     3361 ctcggccccg acgcgcacgc ttcggccaga ccgctctcga cgagcgcgca gagttccggc
     3421 acgtcctccg caggctccgt gccgaaccgc tcgcggtagg cgcccgcgtc cagcccctct
     3481 gcgcgcagca gagacttgac gaggtagcgg cggcgctgct ccgcgccgtc cagggcgaag
     3541 ccgtaggcgg cgcgctcgaa ctcctcgggc gggcaggccg cgtagcgcgc gatgatctcg
     3601 cggacgccgc gcgcccccac ggcatactcg ttcgagtagt gcagcgcgct cgtgtaggag
     3661 cgcgcgccgc agccgatgcc gaccatgccg tcgtcctgac agcagtaggc cgggccttcg
     3721 tcagacggcg cgcgcgccgc gcggaacatc cgcatcgaga cctgctcgta cccttcggcc
     3781 agaagcagcg cgcggccctc gcggtagcac gcgaggcgga tgtcgtccca ctccaacggg
     3841 cggcgttcga gtcccgtcag cgggcgcacg tagagcgggt agaggtacag ctcctccggc
     3901 gcgaagcgga gcgcggcgac gagcgacgcg agccagcttc gcacgctctg tccgggcagg
     3961 ccgtagatca ggtcgatgtt gagcatgggg aaccctgctt cgcggatcag cgcgagcgcc
     4021 gccagcacgt cttccgtcct ctgcgggcgc ccggcggcgc gcgcctccct ctcgtcgaag
     4081 ctctgcacgc cgatgctcaa acgcccgacg ccgcgttcgc gcagcacacg cagccggtcg
     4141 cgctcggcgg tcagcggcga ggtctcgcac gaggcgggga tgtctcgcgc gtcaacgccg
     4201 aaggcgcgcg cggcgatacg gaagagcgag tcgagcgagg ccgggtcgag gtaggtcggg
     4261 gtgccgccgc cgacggccat gcgcgcgaac cgcgccccgc agaggatacg accgaccgcc
     4321 tccgcctgtc gcgcgaaggc tttcaggtac gcgtcgcgcg cctcctcttc agggttggcc
     4381 gtggtgaaga ggttgcagaa cccgcaacgc atctcgcaga aagggacgtg caggtagagg
     4441 aacagagagt ccttcggctc cgcggcccac gcgtcctcaa gcccgaccgc cggctcaaga
     4501 accctgtagg cgctcttgtg cgggtacgag taggcgtagg actcgtaagg cgcacgccgc
     4561 agctttcttt caaggtctgt cacgcgcaca cctccggcgg caggacgaac tcggcgtaag
     4621 gcaccgtcca cacgacgccg tgcgcgacgc ggtgcccgta ataacctcct tcgccgtaag
     4681 ccgtgccgtg gtcggagcac atcacgacga gggccgcgcc gcgccgcgcg agcgcctcga
     4741 agagcggcgg cagttggccg tcaacgtaag cgagggccgc cgcgtgcgac gcgagcgagt
     4801 cgcgctccgc gcccggcagg taaaaacggt tcggctggtg cacggcggag acgttgagga
     4861 agaggaagac tctgcgcccg cgcggcacgc gcgcgagcgt ctccacggcc ctcctgatct
     4921 ggtgctcggt cgagcgcggc tcggcgacgc cgaactccgg ctcccagtgg ctctcgtcga
     4981 agaagccggg gaggacgcga ccgagagggt tctgcttgtt gaagaagccg acgccgccga
     5041 cgcaggcggt gtgatagccg cgcgcggcga ggccgctcac gatgtcgggc gcgtcgaaga
     5101 cgcaggtgcg ctccgaggtg gtctcgctgc cctcgaagcg ggcggcgaag aggcgcgggt
     5161 gggggccggg gccggtcggc gtcggcagga agcccgcgaa gaaggagtgg tgcgcggcgt
     5221 aggtgaagct cgcgggcgtg tgccgctctt cccagccgcc gggcggcagg gcgcgcgcca
     5281 gattcggcgt gcttcccgcg gccagagcgt cgcgcgccac gtcgtaacgc agcgtatcca
     5341 gcgtgatgaa aagcgcgtcg tgcgcaccga tcaagcttcg cgcgtcgagc attccttcaa
     5401 ctccatttcg cataatgccc gcagttcagc ggcgtatgtt tcttgtccct gccagaacac
     5461 gctg
//
LOCUS       DBNGOH010000662         2093 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1931497, whole genome shotgun sequence.
ACCESSION   DBNGOH010000662 DBNGOH010000000
VERSION     DBNGOH010000662.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2093)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2093)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2093
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1931497"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1505)
                     /locus_tag="AC159P_12865"
     CDS             complement(<1..1505)
                     /locus_tag="AC159P_12865"
                     /inference="COORDINATES: protein motif:HMM:NF015531.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HIR3696385.1"
                     /translation="MPRPATIEAFALACSLALLGISARGRQPTTTADARSAQEVAGRY
                     EGVARSRTSGDIPVTAEIRVTTGGDITGVMQTPLGDLSITNGSYAGGTLKFKIESYDD
                     DGTVTARFADGKLNGEFEGFGQSGTIELRRTGPPGPVPDTRPVINLPKEAWREDLRFL
                     ARELPLKHKNAFHLTTRAEFERAVAELDARIPSLSDSERVVHLSRIVAMIGDGHTALG
                     WRWLYPRVPLELFWFGSELRVTRAAKTHRRALGAKLVRIGGAPIADVYARSLPFIPRG
                     ESEGFVRSTHADLSTRPVFLHTLGLAPDNAHARYTFEDERGRTFTLDISAEQDVPAAA
                     WLDAAARTPLYREQPDAPLWFKHLPDSRTLYVNFKGYPRRREFAESSKKLFDFIDRTP
                     VERIVFDLRQNGGGDLTRGRDFIIKQVKERPSVNRRGRLYVVTGRWTYSAGMANAADF
                     RNDTHAILVGEPTGARPNGYQEGRSFTLPNSRLPVYYSIEFYKFQEKDTPG"
     gene            complement(1483..>2093)
                     /locus_tag="AC159P_12870"
     CDS             complement(1483..>2093)
                     /locus_tag="AC159P_12870"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070007 - obsolete glutamic-type
                     endopeptidase activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HIR3696386.1"
                     /translation="TLLSSAIIFLIGAAAEEALFRGYPLQTLMRSWPLWVAALPTSLL
                     FALVHLDNPNVGSAFAFAVVNTALAGVWLAVAYARTRSLWFALGLHWSWNWAMGALFG
                     LPVSGIRDLTPEPLLRAADHGPAWLTGGAYGPEGGAACTAALLLSTLFVWKTNIIAAA
                     EELKHFTDHENPREPDALSLTTPPRAAALIETEDAHAATRND"
BASE COUNT          298 a          623 c          842 g          330 t
ORIGIN      
        1 atgccgggcg tgtccttctc ctgaaacttg tagaactcga tggagtagta gacgggcagg
       61 cgcgagttgg ggagcgtgaa gctgcgcccc tcctggtagc cgttgggacg cgcgccggtg
      121 ggttcgccga cgaggatggc gtgggtgtcg ttgcggaagt cggcggcgtt ggccatgccg
      181 gcggagtaag tccagcggcc cgtgacgacg tagaggcgac cgcggcggtt gacggaaggg
      241 cgctctttga cctgtttgat gatgaagtcg cggccccgcg tgaggtcgcc gccgccgttc
      301 tgcctgaggt cgaagacgat gcgctcgacc ggggtgcggt cgatgaagtc gaagagcttc
      361 ttcgaacttt cggcgaactc gcggcggcgc gggtagcctt tgaagttgac gtagagcgtg
      421 cgcgagtcgg ggaggtgctt gaaccagagc ggcgcgtcgg gctgctcgcg gtagagcggc
      481 gtgcgcgcgg cggcgtcgag ccacgcggcg gcgggtacgt cctgttcggc gctgatgtcg
      541 agagtgaagg tgcggccccg ttcgtcctcg aaggtgtagc gcgcgtgcgc gttgtccggc
      601 gcgaggccga gcgtgtggag gaagacgggg cgggtcgaga ggtctgcgtg cgtcgagcgg
      661 acgaagccct cgctctcgcc gcgcgggatg aaggggaggc tgcgcgcgta aacgtccgcg
      721 atgggggcgc cgccgattcg gacgagcttc gcgccgaggg cgcggcggtg ggtcttcgcg
      781 gcgcgtgtga cgcgcagctc gctgccgaac cagaacagct caagcggcac gcgcgggtag
      841 agccagcgcc agccgagggc ggtgtgtccg tcgccgatca tggcgacgat gcggctcagg
      901 tggacgaccc gttcgctgtc cgaaagggaa gggatgcgcg cgtcgagttc ggcgacggcg
      961 cgctcgaact cggcgcgggt ggtgaggtgg aaggcgttct tgtgtttgag cggaagctcg
     1021 cgcgcgagga agcgcaggtc ttcgcgccag gcttccttcg gaaggttgat gacaggtcgc
     1081 gtgtccggga cggggccggg cgggccggtg cgacgcagct cgatggtgcc gctctggccg
     1141 aagccctcga actcgccgtt gagcttgccg tcggcgaagc gcgcggtgac ggtgccgtcg
     1201 tcgtcgtagc tctcgatctt gaacttgagc gtgccgcccg cgtagctgcc gttcgtgatg
     1261 gaaaggtcgc cgagcggcgt ctgcatcacg cccgtgatgt cgccgcccgt cgtgaccctg
     1321 atctcggcgg tgacggggat gtcgccgctc gtgcggctgc gcgcgacgcc ctcgtaacga
     1381 ccggcgacct cctgcgcgct ccgcgcgtcc gccgtcgtcg tcggctgccg gccgcgagcc
     1441 gagatgccga gcagcgcgag agagcacgcc agcgcgaaag cttcaatcgt tgcgggtcgc
     1501 ggcatgtgcg tcctccgtct cgatgagggc cgcggcgcgg ggcggcgtcg tcaggctcag
     1561 cgcgtcgggt tcgcgcgggt tttcgtggtc ggtgaagtgc ttcagctctt ccgccgcggc
     1621 gatgatgttg gtcttccaga cgaagagggt cgagaggagc agggcggcgg tgcaggcggc
     1681 gccgccttcg gggccgtagg cgccgccggt gagccaggcg gggccgtggt cggcggcgcg
     1741 cagcagcggc tcgggcgtga ggtcgcggat gccgctgacg ggcagcccga agagcgcgcc
     1801 catcgcccag ttccagctcc agtgcagccc gagcgcgaac cagaggctcc gcgtccgcgc
     1861 gtaggcgacc gcgagccaca cgcccgcgag cgccgtgttg acgaccgcga acgcgaaggc
     1921 gctgccgacg ttcgggttgt cgaggtggac gagcgcgaac aggagcgagg tcggcagcgc
     1981 ggcgacccac agcggccacg agcgcatcaa cgtctgcaaa gggtagccgc ggaagagcgc
     2041 ctcttcggcg gcggcgccga tgaggaagat gatggccgaa gagagaagcg tct
//
LOCUS       DBNGOH010000663         5616 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1015973, whole genome shotgun sequence.
ACCESSION   DBNGOH010000663 DBNGOH010000000
VERSION     DBNGOH010000663.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5616)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5616)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5616
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1015973"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(87..1205)
                     /locus_tag="AC159P_12875"
     CDS             complement(87..1205)
                     /locus_tag="AC159P_12875"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013292.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylasparaginase"
                     /protein_id="HIR3696387.1"
                     /translation="MKRRDFLRSGAAAAGVVAAAPQALRGAAPAVITQGAARPVVIAS
                     GNGNRFKNGGDATCVEKAYALMARGADVLDAVIEGVNIVELDPLDDSVGYGGLPNAEG
                     QVQLDASCMHGPKKRAGAVAALEGVRTPSLVAKAVLEQTDHHLIVGHDAQRFARSLGF
                     RVEDDLNTEHSRKLWLEWKRRTDPLHYLDPKTRAEAWHREGLRMVAEGLIDPEHYYGT
                     INCNGVNARGDICGVTTTSGLSWKIPGRVGDSPILGAGLYVDGAVGAAGSTGRGEANL
                     YNLCSFLIVEEMRRGKSPKDAGMEALRRIKANTVEKRLLNSRGTPNFGINFYVVNAKG
                     EHAGVTMYEGSRATYAVCTEAGAKTLTCDGLLPGKPQD"
     gene            complement(1255..2355)
                     /locus_tag="AC159P_12880"
     CDS             complement(1255..2355)
                     /locus_tag="AC159P_12880"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3696388.1"
                     /translation="MRKLILTPALAFALFAVPAFAAQLEAPKLVPVPETEQQIATIRE
                     GVALHDRGDYDGAIRKYEEVLKENPASVRALYELAFAYSLKKNHKKSIEIAYRGAQYK
                     WDEIGLFYQLIGNNLDATGEPKKAIEVYKAGLKHAPRMGLLHFNMAVTYRGMGNPEEA
                     RKSLKNSLALNPGHASSHLLLALTFFQTGYRAPALFAAARFLTLEPATERSGVALRIA
                     QEVMGGGATQGKNPNEINIFMNMNSKKDEGDFGAIEVLLGLSKAVALTEEGKDKNKGK
                     TEAQLLVEQYDTVLAVVGEQTEGKKQSAFVFKYYVPYFVELKQRGFVEPFVHYTHQRV
                     LEGSAQWVAENSGRVMQFLSWSKNYKWPEEAK"
     gene            complement(2368..3963)
                     /locus_tag="AC159P_12885"
     CDS             complement(2368..3963)
                     /locus_tag="AC159P_12885"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HIR3696389.1"
                     /translation="MLPRAFASAVLLLSLGLAHAAARDDYRRLTALDAAHYLIRLEIE
                     EAGEEIGAETEITFAANSDVREVPLDFGGLTIDAVTVDGSAAVSKHGGGKLVVALPRA
                     YRRGETFRVAVRYHGRPADGLFFKRNKFGDRTVFADNWPNRAHHWFPSIDHPYDKATV
                     EFVVVAPARFEVVANGALVERTSRQNGTELTHWRANVPLPVYCMVVGAAEFSVVNAGA
                     WGTTPLVYYLFPRDRDHGLRGFTRALRMLEFYSQLVGPYPYEKMSFVQSSTRFGGMEN
                     SSNVFLDEKAIKEGAAIDGLLAHETAHQWFGDSVTAADWHHLWLSEGFATYFGAHFFE
                     HADGRAAFLKAMQSNRESYLKATAEHARPIIDPAITDIFKLLNRNSYAKGAWVLHMLR
                     RELGDEKFFAGVREYYRAHRDSTALTEDFRRALELHAGRTLEPFFRQWIYEPGHPVID
                     AAWHWDESSKRLRLRLRQTQTPTTFHFPLDVEIATGDGSRREAIEVNAREQSFDFKLD
                     ARPDGLVLDPDEWLLKTLSLRQE"
     gene            complement(3981..4382)
                     /locus_tag="AC159P_12890"
     CDS             complement(3981..4382)
                     /locus_tag="AC159P_12890"
                     /inference="COORDINATES: protein motif:HMM:NF024966.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR3696390.1"
                     /translation="MSTETAHTDEREAILRVERDTMAAIRRRDVEALGRILADDFVYR
                     TPGKRDAGRAEFLNHIASIPVEILSVWGDELHVLVNGETAVLTGVQRAKYRTCEGAEG
                     VSSVAFADVLVKRGGRWLMVLAHGVELPDAT"
     gene            4647..4994
                     /locus_tag="AC159P_12895"
     CDS             4647..4994
                     /locus_tag="AC159P_12895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696391.1"
                     /translation="MLSRRSFVLPALLAGVLAFAGCPSRTTIGKINADPERYFDKEVG
                     VVGRVTDSYGVPFVGGAYEIDDGTGKIWVLTERGAPSKGSQVGVKGRVVNGPVYKGRN
                     LGTALRETDRRVK"
     gene            5050..5529
                     /locus_tag="AC159P_12900"
     CDS             5050..5529
                     /locus_tag="AC159P_12900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696392.1"
                     /translation="MLEIVRSLDEHFARLHERSAALVRGVPADKLYWQPRASSGSFPV
                     YSCGEHVLRSAAAVEQTCGGIAVNLWDDPFEWTLPEALPTGERVVEYLDEAEATRRRA
                     FALFDSDQDLLREIAAPPGEMRTLFSLLTETLVRAAHHQGRAFATFRLFSEGRLPRV"
BASE COUNT          844 a         1862 c         1904 g         1006 t
ORIGIN      
        1 acggaagaaa agagaggctt gggactttac ctcccaagcc tcttcgcctt ctgtcatcac
       61 tgcctactgc ctaccgcctg ctgccttcag tcctgcggct tgccggggag caggccgtca
      121 cacgtcagcg tcttggcgcc cgcctcggtg cagacggcgt aggtggcgcg cgagccttcg
      181 tacatggtga cgcccgcgtg ctcgcccttc gcgttgacga cgtagaagtt gatgccgaag
      241 ttgggcgtgc cgcggctgtt cagcagacgc ttctcgacgg tgttggcctt gatgcggcgc
      301 agggcttcca tgcctgcgtc tttgggcgac ttgccgcggc gcatctcctc gacgatgagg
      361 aacgagcaga ggttgtagag gttcgcctcg ccgcgcccgg tggaacccgc cgcgccgacc
      421 gcgccgtcca cgtagaggcc cgcgccgagg atgggcgagt cgcccacgcg ccccggtatc
      481 ttccaagaca gcccgctcgt cgtcgtgacg ccgcagatgt cgccccgcgc gttgacgccg
      541 ttgcagttga tcgtgccgta ataatgctcc gggtcgatca gaccttcggc gaccatccgc
      601 agcccttcgc ggtgccaagc ctcggcgcgc gtcttcgggt cgaggtagtg cagcgggtct
      661 gtgcgccgct tccattcgag ccagagcttg cgcgagtgtt cggtgttgag gtcgtcctcg
      721 acacggaagc cgagcgagcg cgcgaagcgc tgcgcgtcgt gcccgacgat gaggtggtgg
      781 tctgtctgct ccaacaccgc cttcgccacc agcgacggcg tccgcacgcc ttcgagcgcg
      841 gccaccgcgc ccgcgcgctt cttcggcccg tgcatgcacg acgcgtcgag ctgcacctgc
      901 ccttcggcgt tcggcagccc gccgtagccg acgctgtcgt ccagcgggtc gagttcgacg
      961 atgttcacgc cctcgatcac ggcgtcgagc acgtccgcgc cccgcgccat caacgcgtaa
     1021 gccttctcga cgcacgtcgc gtcgccgccg ttcttgaacc tgttcccgtt ccccgaggcg
     1081 atgacgaccg gcctcgccgc gccctgcgtg atgaccgccg gggccgcgcc ccggagcgcc
     1141 tgcggcgcgg cggcgaccac acccgcggcg gcggcgccgc tcctcagaaa atcacgccgt
     1201 ttcattcttt tgcccatgac ctgcctccga aattcaagcg ctcctcggcg ccgctcactt
     1261 cgcctcttcg ggccacttgt agtttttcga ccacgagagg aattgcatga cccgcccgct
     1321 gttctccgcg acccactggg cggagccttc gagcacgcgc tggtgcgtgt aatggacgaa
     1381 cggctcgacg aagccgcgct gcttcagctc cacgaagtac gggacgtaat acttgaagac
     1441 gaaggccgac tgcttcttcc cctccgtctg ctcgccgacg acggcgagca ccgtgtcgta
     1501 ctgctcgacg agcagttgcg cctcggtctt gcccttattc ttatccttcc cctcctccgt
     1561 caacgccacc gccttcgaca ggccgagcag gacttcaatc gcgccgaagt cgccctcgtc
     1621 cttcttggag ttcatgttca tgaagatgtt gatctcgttc gggttcttcc cctgcgtggc
     1681 gccgccgccc atgacctcct gcgcgatgcg gagggcgacc cccgagcgct ccgtcgccgg
     1741 ttcgagcgtc aggaagcggg ccgcggcgaa gagcgcgggc gcgcggtagc cggtctggaa
     1801 gaaggtcagc gccagcagca ggtggctgct ggcgtggccg ggattcaggg ccagcgagtt
     1861 cttcagactc ttccgcgcct cctcgggatt gcccatgccc ctgtacgtga cggccatgtt
     1921 gaagtggagg aggcccatgc ggggcgcgtg cttgaggccg gccttgtaga cttcgatggc
     1981 cttcttcggc tcgccggtcg cgtccaaatt gttgccgatg agctggtaga agaggccgat
     2041 ctcgtcccac ttgtattgcg cgccgcggta ggcgatttcg atactcttct tgtgattctt
     2101 cttcagcgag taggcgaagg ccagctcgta gagcgcgcgg acgctcgccg ggttctcctt
     2161 caatacctcc tcgtacttgc ggatggcgcc gtcgtagtcg cctcggtcat gcagggcgac
     2221 gccttcgcgg atcgtcgcga tctgctgctc ggtctcgggg acgggcacca gtttcggcgc
     2281 ttcgagctgg gcggcgaagg ccgggaccgc aaagagcgcg aaggccagcg cgggggtcag
     2341 gattagttta cgcatacacg ctcgctttca ttcctgcctg agtgacaggg ttttcagcag
     2401 ccactcgtcg gggtcgagca cgaggccgtc gggtcgtgcg tcgagcttga agtcgaagga
     2461 ctgttcgcgc gcgttgactt cgattgcctc gcgccgcgaa ccgtcgccgg ttgcgatttc
     2521 gacatcgagc gggaagtgga aagtggtcgg cgtctgcgtc tggcgcaggc ggaggcggag
     2581 gcgcttcgaa gattcgtccc agtgccacgc ggcgtcgatg acggggtggc ccggctcgta
     2641 aatccattgg cggaagaagg gttcgagcgt gcggcccgcg tgcagctcca gcgcgcggcg
     2701 gaagtcttcg gtgagcgccg tcgagtcgcg gtgggcgcgg tagtattcgc gcacgcccgc
     2761 gaagaacttc tcgtcgccca gctcgcgccg cagcatgtgg agcacccacg cgcccttcgc
     2821 gtagctgttc cggttgagca gcttgaagat gtccgtgatc gcggggtcga tgatggggcg
     2881 cgcgtgctcg gcggtcgcct tgaggtagct ctcgcggttc gattgcatcg ctttcaggaa
     2941 cgccgcgcgc ccgtcggcgt gctcgaagaa gtgcgcgccg aagtaggtgg cgaagccctc
     3001 gctcagccag aggtggtgcc agtcggcggc ggtcacggag tcgccgaacc actggtgcgc
     3061 ggtctcgtgc gcgagcagtc cgtcgatggc ggcgccttcc ttgatcgcct tctcgtcgag
     3121 gaagacgttg ctcgaattct ccatcccgcc gaagcgcgtg gacgactgga cgaaggacat
     3181 cttctcgtaa gggtacgggc cgacgagctg cgagtagaat tcgagcatcc ggagcgcgcg
     3241 cgtgaagccg cgcaggccgt ggtcgcggtc gcgcgggaag aggtagtaga cgagaggcgt
     3301 cgtgccccac gcgcccgcgt tgacgacgga gaactccgcg gcgccgacga ccatgcagta
     3361 gacgggcagc ggaacgttcg cgcgccagtg cgtcagctcc gtgccgttct gcctgctcgt
     3421 ccgttcgacg agcgcgccgt tggcgaccac ttcgaagcgc gcgggcgcga cgacgacgaa
     3481 ctcgaccgtg gccttgtcgt acgggtggtc gatggagggg aaccagtggt gcgcgcggtt
     3541 gggccagttg tcggcgaaga ccgtgcggtc gccgaacttg ttccgcttga agaagagtcc
     3601 gtccgcaggc cgcccgtggt agcggacggc gacgcggaag gtctcgccgc ggcggtaggc
     3661 gcgcgggagc gcgacgacga gcttgccgcc gccgtgtttc gaaacagcgg cgctcccatc
     3721 gaccgtcacc gcgtcgatgg tcagcccgcc gaagtcgagc gggacttcgc gcacgtcgga
     3781 gttggccgcg aaggtgatct cggtctcggc gccgatctct tcgcccgcct cttcgatttc
     3841 gaggcggata aggtagtgcg ccgcgtcgag cgccgtcagc cgccggtagt cgtcgcgcgc
     3901 cgccgcgtgc gcgaggccga gcgacagcag cagcacggcg gacgcgaaag ctctcggcag
     3961 catcatcaag cgaggctcct tcaagtcgcg tcgggcagct cgacgccgtg cgcgagcacc
     4021 atcagccagc gcccgccgcg cttgacgagc acgtcggcga aggcgacgga gctgacgccc
     4081 tcggcccctt cgcacgtgcg gtacttcgcg cgctgcacgc ccgtcagcac cgccgtctcg
     4141 ccgttgacga ggacgtgaag ctcgtcgccc cagaccgaaa gtatctcgac ggggatggag
     4201 gcgatgtggt tcagaaactc tgcccggccc gcgtcccgtt tgcccggcgt gcggtagacg
     4261 aagtcgtctg cgaggatgcg gcccagagcc tcgacgtccc tgcgccggat ggccgccatc
     4321 gtgtcgcgtt cgacccggag gatcgcctcg cgttcgtcgg tgtgcgctgt ctctgttgac
     4381 acctcacgcc cccggaatgg aaagttgtgt ggaacgtcgc agcgaagggg ccgcgcccgt
     4441 cgggcggcgg cgcgttaacg taccatagaa acgccgataa aaagaagtcg gaggtcagag
     4501 gtcggaggtc ggaataaaaa ctcttctctt ctgacctccg acctccgacc tctgacctct
     4561 gtctcagggt gctttttccc ccggcaactc cgcgtgctat gatggccgcg ctctcccgga
     4621 attcgattgg aaggaaggag gagcagatgc tctcaagacg atcattcgtg ttgccggcgc
     4681 tactggccgg cgtgctcgct ttcgcgggct gcccgagccg caccaccatc gggaagatca
     4741 acgcagaccc ggagcgctac ttcgacaagg aggtcggcgt cgtcgggcgt gtgacggaca
     4801 gctacggcgt gccgttcgtc ggcggcgcct acgagatcga cgacgggacg ggcaaaatct
     4861 gggtgctcac ggagcgcggc gcgccctcga aggggtcgca ggtcggcgtc aaggggcgcg
     4921 tcgtgaacgg ccccgtctac aagggccgca acctcggcac agccctgcgc gagaccgacc
     4981 gccgcgtcaa gtagcccctc agccgggcgg cgcggagggc gcgggcgatg gacgaagcgg
     5041 gtgccgccga tgcttgagat cgtccgcagc ctcgacgaac acttcgcgcg cctgcacgag
     5101 cgctcggcgg cgctcgtgcg gggcgtcccc gccgacaaac tctactggca gccgcgcgcg
     5161 tcttcgggca gcttcccggt ctactcctgc ggcgagcacg tcctcagaag cgccgccgcc
     5221 gtcgaacaga cttgcggcgg catcgcggtc aacctctggg acgacccgtt cgagtggacg
     5281 ctgcccgaag cgctgccgac gggcgagcgg gtcgtcgaat atctggacga ggccgaggcg
     5341 acgcgccgcc gcgcgttcgc gctcttcgac tccgaccaag acctcctgcg cgagatcgcc
     5401 gcgccgcccg gcgagatgcg cacgctcttt tcgctgctga ccgaaacgct cgtgcgcgcc
     5461 gcgcaccatc agggccgcgc cttcgccacc tttcgattgt tctcggaagg gcgcctgccg
     5521 cgcgtctgac ggggcggggc ggcgaacttc ggcgccgcga ctttcgtagt tgagcgcgag
     5581 gcgcataggt ttataatcgc gcgttggctt aacgac
//
LOCUS       DBNGOH010000664         5148 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2482083, whole genome shotgun sequence.
ACCESSION   DBNGOH010000664 DBNGOH010000000
VERSION     DBNGOH010000664.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5148)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5148)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5148
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2482083"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2008
                     /locus_tag="AC159P_12905"
     CDS             <1..2008
                     /locus_tag="AC159P_12905"
                     /inference="COORDINATES: protein motif:HMM:NF012801.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR3696393.1"
                     /translation="QQVRVAAGAPATHDFQLKLTGYREEVTVTATGSEQSTFESFQAV
                     TTLDSIRIGEESHTALGEVLDKEPGVAKRSFGPGTSRPVIRGFDGDRVLVLQDGVRTG
                     SLGSQSGDHGEPVDVLSLERLEVVKGPATLLYGSNAVGGVVNAVTGHDYAHEGWRGYF
                     TGLAGTTNSQAGTSGGIEYGTGRWMLWGNGSFQRTGDYDTPEGRVPNSQTRSLYGLGG
                     FGWYGDKTFFSASYSHDNRRYGVPFAASFEGEEEEEANIARAPGGGNLFADQLQEEDE
                     QIDLKMRRHDFQINTGFRNLASFVDSFRLTLDYSDYRHQELEGDAVGTTFNNKQFVYR
                     GVFSQRKTGKFSGSFGFSGFHRDYETIGAEALAPPVVQNSFAVFGLEAIEFERVGFQL
                     GARVERNSYNPDALQDRAFTGFSGAAGVRFDLWRGGAFVANYTHSYRAPALEELYNNG
                     PHVGNLSFEIGNPNLTRERNDGIDFSLRHRSPRVRAEANFYYYNLQDFVFLAPLDEDG
                     DGEIDIEDGLRVAEYRQGDSRFVGTELDLDVALHEYLWLNAGLDAVDAQLKETGTPLP
                     RIPPLRARFGLDARYKGLNLRPEAVFVKDQDDLFTTETRTPGYTVFNLVASYTIARPH
                     YAHIFSVNAFNLGDRLYRNHLSFIKDLAPEIGRGIRFTYTIRYF"
     gene            2392..3702
                     /locus_tag="AC159P_12910"
     CDS             2392..3702
                     /locus_tag="AC159P_12910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M61 metallopeptidase family protein"
                     /protein_id="HIR3696394.1"
                     /translation="MTATETKAWSLPAARVTLKGFGERVSNLTLHDAAGAEVPVRKLA
                     PGEFEAARAASKFSYDLNLDPPFVTSDAAHVSWLSGGRGIIFLSDVLPVLPAKGRATF
                     KLPAGWQIFSVEPKDREGNYEVTDLLRSLFVIGRDLRDRSGQVNSMDVSYVTAGEWAF
                     PDDELIETSKETLDDYRKVLGAMPRRRSTVVLMPFPTQVAGHVWSAETRGGTVLLLSG
                     HLPSKTAALAQLNGALTHELFHLWVPNGLALDGEYGWFYEGFSLYQALRTGTRQGRLT
                     FQDYLTAMGRAFDGYKSARGRQELSLVEASERRLTGNTALVYHKGMLVAFLYDLMLMR
                     RTEGKSSLDEVYRELYRRYGQRETRENASRAVVSALGAMPGMQGFVDNYISNPTRIEL
                     KDEIEVFGLRVEPGGIRTRVVVSDSITRQQRDLLRRLGYNGRRG"
     gene            3881..4957
                     /locus_tag="AC159P_12915"
     CDS             3881..4957
                     /locus_tag="AC159P_12915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696395.1"
                     /translation="MLGTGCSTLAGKEDTCVVMARRAQVRSSTAVVAADLKEVVRGDT
                     LDILDSITVPETGESWLRVRVRDEEGTEGWIEARNVISQQMVERSRGIAKDDESIPAQ
                     ASGQLRASTNIRFSPDRSGNDNILMKLESGARFEIVGWKRVPRPKSLDTTESDDAPKA
                     GAAEPGTVRRKRESDRPKEPEDVNEIWYKVRLPPSISPAPAGWVFGKQVELTVPSDII
                     FYRTGREFVAWQRLDAEGDDLQKASLRDKDAAKDARPGSWVILEKSSTGEANSLDSPD
                     FDRIFVLGYDKDRQEHYTAYRSPDLNGFLPLRIERNGEVKNFAVRVKVDDQFKEVKFK
                     AYLNERGLLKVEALDELPKVNKPR"
BASE COUNT          979 a         1736 c         1558 g          875 t
ORIGIN      
        1 gcaacaggtg agggtcgccg cgggcgctcc ggccacgcac gactttcagt tgaagctgac
       61 cggctaccgc gaagaggtga cggtgacggc gacggggagc gagcagtcca cgttcgaatc
      121 gtttcaggcc gtgaccacgc tcgactcgat ccgcatcggc gaggagagcc acacggcgct
      181 cggcgaagtg ctcgacaaag agccgggcgt cgccaagcgc agcttcgggc cgggcacttc
      241 gcgccccgtc atccgcggct tcgacggcga ccgcgtgctc gtcctgcaag acggcgtgcg
      301 cacaggctcg ctcggctcgc agtccggcga ccacggcgag cctgtggacg tgctctcgct
      361 cgaacgcctc gaagtcgtca aaggcccggc gacgctgctc tacggcagca acgccgtcgg
      421 cggcgtggtc aacgccgtca ccggccacga ctacgcgcac gagggctggc gcggctactt
      481 caccggcctc gccggcacga cgaacagcca ggccggcacg agcggcggca tcgagtacgg
      541 cactggccgc tggatgctgt ggggcaacgg cagcttccag cgcacgggcg actacgacac
      601 gcccgagggg cgcgtgccca actcgcagac gcgcagcctc tacggcctcg gcggcttcgg
      661 ctggtacggc gacaagactt tcttcagcgc cagctacagc cacgacaacc gccgctacgg
      721 cgtcccgttc gccgcctcgt tcgagggtga agaggaagag gaagcgaaca tcgcgcgcgc
      781 gcccggcggc ggcaacctct tcgccgatca actgcaagag gaagacgagc agatcgattt
      841 gaagatgcgg cgccacgact ttcaaatcaa caccggcttc cgcaacctcg cctcgttcgt
      901 cgattctttc cggctgacgc tcgactacag cgactaccgg catcaggagc ttgagggcga
      961 cgcggtcggc accaccttca acaacaagca gttcgtctac cgcggcgtct tctcgcagcg
     1021 caagaccggg aagttctccg gcagcttcgg cttctccggc ttccaccgcg actacgaaac
     1081 catcggcgcc gaagccctcg cgccgcccgt cgtccaaaac tctttcgccg tcttcggcct
     1141 tgaggccatc gagttcgagc gcgtcggctt ccaactcggc gcccgcgtcg agcgcaacag
     1201 ctacaacccc gacgccctgc aagaccgcgc cttcacgggc ttctccggcg ccgcgggcgt
     1261 ccgcttcgac ctctggcggg gcggcgcctt cgtcgccaac tacacgcact cgtaccgcgc
     1321 gcccgcgctc gaagagcttt acaacaacgg cccgcacgtc gggaacctct ccttcgagat
     1381 cggcaacccg aacctcacgc gcgagcgcaa cgacggcatc gacttctcgc tccgccaccg
     1441 ctcgccgcgc gtccgcgccg aggcgaattt ctactactac aaccttcagg acttcgtctt
     1501 cctcgcgccg ctcgacgagg acggcgacgg cgagatcgac atcgaagacg gcctgcgcgt
     1561 cgcagaatac cggcagggcg acagccgctt tgtcggcacc gaactcgacc tcgacgtggc
     1621 gctgcacgag tacctctggc tcaacgcggg gctggacgcc gttgacgcgc agttgaagga
     1681 gaccggcacg ccgctgccgc gcatcccgcc gctgcgcgcg cgcttcggcc tcgacgcgcg
     1741 ctacaaaggc ctgaacctgc ggcccgaggc cgtcttcgtc aaagaccagg acgacctctt
     1801 cacgaccgag acgcgcacgc ccggctacac cgtcttcaac cttgtcgcgt cgtacacgat
     1861 tgcgcgcccg cattacgcgc acatcttctc ggtcaacgcc ttcaacctcg gcgaccgcct
     1921 ctatcgcaac cacctctcct tcatcaaaga cctcgcgccc gagatcggtc gcggcatccg
     1981 cttcacctac accatccgtt acttctaaca ggcgacaccc ggagcgcacg aaacatcacc
     2041 gggcgcggcg atggttgaac catcgccgcg ccccttcttt tcacaccgcg ccagcgacag
     2101 accggcgacc gtagttccca cactccgcaa acgcccgcgc ctgtggttaa atgaatgcgc
     2161 cacaactcga ccgcggaagg agttcgcgca cacttgcctt cgaccagacg ctttcgaaga
     2221 agcacgtccg ccacggcttc ggtttttagc aaacgacctt tcatcagcgt ctccgcctgc
     2281 tgcctccttt tcgtcgcgta ccttttaacc acagccactg cccgcgccga ctctttcgac
     2341 gtaaccatca cggtcgtctc gcccgacgcg tcccgcgtcc gcgtcgaagg cgtgaccgcg
     2401 acggagacga aagcgtggtc gctccccgcc gcccgcgtca ccttgaaggg cttcggcgaa
     2461 agagtctcga acctgactct tcacgatgcc gcgggcgccg aggtgcccgt ccgcaaactc
     2521 gcccccggcg aattcgaagc ggcgcgggcc gcgtcgaagt tcagctacga cctcaacctc
     2581 gaccctccct tcgtcacgtc cgacgcggcc cacgtctcct ggctctcggg cggtcgcgga
     2641 atcatctttc tcagcgacgt gctgcctgtc ctcccggcga agggtcgggc gacgttcaag
     2701 ctgccggcgg gctggcaaat tttttccgtc gagccgaaag acagggaggg gaattacgaa
     2761 gtcaccgacc tgcttcgctc cctcttcgtc atcggtcgag acctgcgcga ccgcagcggg
     2821 caggtgaatt cgatggacgt gtcttacgtg accgcgggcg agtgggcctt ccccgacgac
     2881 gagttaatcg aaacgtcgaa ggagaccctc gacgattacc gcaaggtgtt gggcgcgatg
     2941 ccgcgccgcc gctcgacggt cgtgctcatg ccgttcccca cgcaggttgc gggtcacgtc
     3001 tggagcgccg agacgcgtgg cgggacggtg ctccttctgt ccgggcacct cccttcgaag
     3061 acagccgcgc tcgcgcaact gaacggggcg ctgactcacg agctgtttca cctctgggtg
     3121 ccgaacggcc tcgcgctcga cggcgaatac ggctggttct atgaaggctt ctcgctctat
     3181 caggcgctcc gcacggggac gcggcagggc cgtttgacct ttcaggatta tttgaccgcg
     3241 atgggccgcg cgttcgacgg gtacaagtcc gcgaggggcc ggcaggaact ctctctcgtc
     3301 gaggcgtcgg aacgaaggtt gacgggcaac acggccctcg tctatcacaa gggcatgctt
     3361 gtcgcgttcc tctacgacct gatgttgatg cgccgaaccg aaggcaagag ttcgctcgac
     3421 gaagtgtatc gtgagctgta ccggcgttac ggccagcgag agacgcgcga gaacgccagc
     3481 cgcgccgtcg tctccgccct cggcgcgatg cccggcatgc agggcttcgt cgataattac
     3541 atctcgaacc cgacacgaat cgagttgaag gatgagatcg aagtcttcgg cctgcgagtc
     3601 gagccgggcg gcatccgcac gcgcgtcgtg gtctccgatt cgatcacacg ccagcagcgc
     3661 gatttactca ggcgtttagg ctacaatggg cggcgaggct aaggggccaa gcttcgacgg
     3721 attcgggagt tgatcgttat cgcactgccg aactcgaacc gccgacaatc ggcccgagag
     3781 gtataaggcg gacaaactcc gccctccaac gtctctcggc ggaatgaaag acatctcaag
     3841 tatgaagccc gtaaggatca cctttttcat cgctctctta atgctcggaa ccggttgcag
     3901 tacgctcgcg gggaaggaag acacctgcgt cgtcatggcg cgccgcgcgc aggttcgcag
     3961 ctcgactgcg gtcgtggcgg cggacttgaa ggaggtcgtg cgcggcgaca cactggacat
     4021 cctcgattcg atcacggtgc cggaaaccgg cgagagctgg ctccgcgtcc gcgtccgcga
     4081 tgaagagggc accgagggct ggatcgaggc gcgcaacgtc atctcgcaac agatggtcga
     4141 gcgctcgcgc ggtatcgcca aagacgacga gtccatcccc gcccaagcct ccggccaact
     4201 ccgcgccagc acgaacatca ggttctcgcc cgaccgcagc ggcaacgaca acatcctcat
     4261 gaagcttgag agcggcgcgc gcttcgagat cgtcggttgg aagcgcgtgc cgcgccccaa
     4321 gtcgctggac acgactgaga gcgacgacgc gccgaaggcc ggcgccgccg aaccgggaac
     4381 cgtcaggcgc aagcgggaga gcgacaggcc gaaagagccg gaggatgtca acgagatttg
     4441 gtacaaggtg cgtctgccgc cttcgatctc gccggcgccc gccgggtggg tcttcggcaa
     4501 acaggtggag ctgaccgtcc ccagcgacat catcttctac cgcacagggc gcgagttcgt
     4561 cgcctggcag agactcgacg cggaaggcga cgacctccaa aaggcttcgc tcagagacaa
     4621 ggacgccgcg aaagacgctc gccccggaag ctgggtgatc cttgagaaga gcagcacggg
     4681 cgaggccaac agcctcgact cgcccgactt cgaccgcatc ttcgtcctcg gctatgacaa
     4741 ggacaggcag gagcactaca ccgcctaccg cagccccgat ttgaacggct tccttccgct
     4801 ccgcatcgaa cgcaacggcg aggtgaaaaa ctttgccgta agagtcaagg tcgatgatca
     4861 gttcaaagag gtgaagttca aggcgtattt gaatgagcga ggcttgttga aggtggaggc
     4921 actggacgaa ctgccgaagg ttaataaacc ccgctgaaat tgggcattgt ttacgttttg
     4981 aatttttgtc gcaagagtag ttgacaaatg aatctttatg gctataatgc ctctttgcct
     5041 gagacacggc agttgacatc ccggtgacaa agagcgcgtt tgacgagccg cttctctttc
     5101 aagtcttctg acgagaggga acggcttttt catcggcttc ggttcgag
//
LOCUS       DBNGOH010000665         3750 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3580217, whole genome shotgun sequence.
ACCESSION   DBNGOH010000665 DBNGOH010000000
VERSION     DBNGOH010000665.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3750)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3750)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3750
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3580217"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..177
                     /locus_tag="AC159P_12920"
     CDS             <1..177
                     /locus_tag="AC159P_12920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013579277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HIR3696396.1"
                     /translation="GGGALLKNLDKRLSIETGLPVSIADDPLSSVVLGAGKMLSDFDL
                     LKRVKWDNTMVAGM"
     gene            303..1202
                     /gene="mreC"
                     /locus_tag="AC159P_12925"
     CDS             303..1202
                     /gene="mreC"
                     /locus_tag="AC159P_12925"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HIR3696397.1"
                     /translation="MARSTQKEIRQRAPVLLIVLLAVNLGLMTWDARDAVTKQRMIRV
                     WAQAVASPFQRGVTGAGGAGLGFFQRLGNLWRASGENEGLRQRLAEVENELRDARQAR
                     DENERLKKLLGFKEGAAYGVIPAVVIARDPSVWFNSVHINRGTMSGVDVNMPVATPEG
                     IVGRVVAVGPVSAQVMLLTDERAAAGAVVGQLGGSNAIGSVRGYGKNGLLEMRYVSGL
                     EPVNLGDYVVTTGQDGIYPPGLNVGTVVQKTDGSATTPHTIYVKPGARLDSLEEVAVL
                     QYRPPPRTAPERTLPNVDKGKKP"
     gene            1327..1872
                     /gene="mreD"
                     /locus_tag="AC159P_12930"
     CDS             1327..1872
                     /gene="mreD"
                     /locus_tag="AC159P_12930"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HIR3696398.1"
                     /translation="MKWKIAVCVALAVVLQSSLRKFWGPLAYADLPLIVVVYFALRRD
                     AVQAVVIGTVAGLATDVMSGGLLGANGFSKTLTAYFIAAIITRIMLDNPLLRVPVLAG
                     AAAFDSLVYVLLHRLLDQPATPMAGTLAETGAFRVIWTTVAGTVIAYALDIFFSDTAR
                     QRRQFAFRRRIARRGLSRRKY"
     gene            2005..>3750
                     /gene="mrdA"
                     /locus_tag="AC159P_12935"
     CDS             2005..>3750
                     /gene="mrdA"
                     /locus_tag="AC159P_12935"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HIR3696399.1"
                     /translation="MKFEDETQNLRARMTIIQGVVLAILVVLGVRLYFLQVVNGDYYA
                     ERAENQRVRLLRIPAPRGAIFDRNGKLLVDSRSTFNVILSREDLKGKDYNALVQSLVE
                     PLSEALGVDADILRERFEQMRSQPASEPVTIKEGAAHADIAWVESHMLEYPMLSVREQ
                     PQRRYPADGVLAHVLGYVGEISPEQLKQEKYKDYAENPYHAGDIIGQEGLESYYDRLL
                     RGRDGYRKVEVDSRGRIQRELETVPPQPGQDLLTTIDLDMQMAAEKYLANTPSKRGVI
                     VAMNPTNGEILSLASYPTFDPNLFSQRIGTPEGRREANALLRDTRNPLYNRAIKGRYP
                     PGSTWKIPMAMSGLEQGAITVKNSNLACGGGITIGNKFTRCMGSHGAPPVRFAIQVSC
                     DGYFYRLGLKMKLEGIQKMVEDWEINKKTGVDLPRELVSWTPSREFKRRFNKADPEWR
                     DIDTVYASFGQVHEFVTPLAMLRAVAGIGMGGKIYVPHLLKEARAVAAVGPFAARPAV
                     GFARPEPKIVPLSVENHQMVMHGMSDVVNAGGTGGRIKMEGFEIAGKTGTAQVVSLGK
                     DVGANKDHAWFVSFAP"
BASE COUNT          647 a         1225 c         1274 g          604 t
ORIGIN      
        1 ggcgggggtg cgctgctgaa gaacctggac aagcggctgt cgatcgagac ggggctgccg
       61 gtctcgatcg cggacgaccc gctctcgtcg gtggtgctcg gggcgggcaa gatgctctcc
      121 gacttcgacc tcctcaaacg cgtcaagtgg gacaacacga tggtcgcggg aatgtaagag
      181 ccgtgaaccg tgaaccgtaa accgtgacaa gtgaagacaa ggttttcctt gccatggttc
      241 gcagttcacg gttcagggtt ttcacttatc acggtttacg gttcacggtt cacggctctt
      301 ctatggccag gagcacgcag aaagagatac ggcagcgggc gccggtgttg ctgatcgtcc
      361 tgctggcggt gaacctcggg ctgatgacgt gggacgcgcg cgacgcggtg acgaagcagc
      421 gcatgatccg cgtctgggcg caggccgtgg cctcgccgtt ccagcgcggc gtgacgggcg
      481 cgggcggcgc cggcctcggt ttcttccagc gcctcgggaa tctgtggcgc gcgtcgggcg
      541 agaacgaggg gctgcggcag cgcctcgccg aggtcgagaa cgagctgcgc gacgcgcggc
      601 aggcgcgcga cgagaacgag cggctgaaga agctcttggg cttcaaggag ggcgcggcct
      661 acggcgtcat cccggcggtc gtcatcgcgc gcgacccctc ggtgtggttc aactccgtcc
      721 acatcaaccg cgggacgatg agcggcgtgg acgtgaacat gccggtcgcc acgcccgagg
      781 gcatcgtcgg gcgcgtcgtg gcagtcggcc ccgtctcggc gcaggtcatg ctgctgacgg
      841 acgagcgcgc ggcggcgggc gctgtggtcg ggcagctcgg cgggtcgaac gccatcgggt
      901 cggtgcgcgg ctacggcaag aacgggctgc tggagatgcg ctacgtgtcg gggctggagc
      961 cggtcaacct cggcgactat gtggttacca cggggcagga cggtatctac ccacctggcc
     1021 tgaacgtcgg cacggtcgta caaaagaccg acgggtcggc caccacgccg cacacgatct
     1081 acgtcaagcc gggcgcgcgg ctcgactcgc tcgaagaggt cgccgtcctt cagtaccgcc
     1141 cgcccccgcg caccgcgccg gagcggacgc tgccgaacgt ggacaagggg aaaaagccgt
     1201 gaaccgtgaa ccgtaaatcg tgaaggctgg ttggacggca atgacgcctg gatggcaact
     1261 cgcggcggcg cgccgcacac accgcgttta cggtttacgg tttacggttt acggtttacg
     1321 gcctttatga aatggaagat tgccgtttgc gtcgcgctgg ccgtggtgtt gcagtccagc
     1381 ctgcgcaagt tctgggggcc gctcgcgtac gccgacctgc cgctcatcgt ggtggtctac
     1441 ttcgccctgc ggcgcgacgc cgtgcaggcg gtggtgatcg ggacggtggc gggtttggcg
     1501 accgacgtga tgagcggcgg cctgctcggc gccaacggct tctcgaagac gctgacggcc
     1561 tacttcatcg cggccatcat cacgcgcatc atgctcgaca acccgctgct gcgcgtgccg
     1621 gtgctggcgg gcgcggcggc cttcgactcg ctggtctacg tgctgctcca ccggctgctc
     1681 gaccagccgg cgacgccgat ggccggcacc ctcgccgaga ccggcgcctt ccgcgtcatc
     1741 tggacgaccg tcgccggcac cgtcatcgcc tacgcgctcg acatcttctt cagcgacacg
     1801 gcccgccagc ggcgccagtt cgccttccgc cgtcgcatcg cgcgaagggg cctcagccgg
     1861 aggaagtatt gaccgatttc ggattttcgg attttcggat tttggattga cggttgacgc
     1921 ggcgaacttc cagccgccgt ttcggaatct taaaaccgac ttcgtcaacc aagaatcaag
     1981 aaccgcgaat cgaagttaac gacgatgaaa ttcgaagacg agacacagaa cctgcgggcg
     2041 cggatgacga tcatccaggg ggtggtgctc gccatcctcg tcgtgctcgg cgtgcggctc
     2101 tacttcttgc aggtcgtgaa cggcgactac tacgccgagc gggccgagaa ccagcgcgtc
     2161 cggctgctgc gcatcccggc gccgcgcggc gccatcttcg accgcaacgg caagctcctc
     2221 gtcgattcgc gctcgacctt caacgtcatc ctctcgcgcg aggacttgaa gggcaaggac
     2281 tacaacgcgc tcgtccagtc gctcgtcgag cctttgagcg aggccctcgg cgtggacgcc
     2341 gacatcctgc gcgagcgctt cgagcagatg cgctcgcagc ccgcctccga gccggtcacg
     2401 atcaaggagg gcgccgcgca cgccgacatc gcctgggtcg agtcgcacat gctggagtac
     2461 ccgatgctca gcgtccgcga gcagccgcag cgccgctacc cggcggacgg cgtgctcgcg
     2521 cacgtcctcg gctacgtcgg cgagatcagc cccgagcagc tcaagcagga gaagtacaag
     2581 gactacgccg agaaccccta ccacgccggc gacatcatcg ggcaggaggg tttggaatcc
     2641 tactacgacc ggctgctgcg cgggcgcgac ggctaccgca aggtcgaggt cgatagccgc
     2701 gggcgcatcc agcgcgagct tgagaccgtg ccgccgcagc ccggccagga cctgcttacg
     2761 accatcgacc tcgacatgca gatggccgcc gagaagtacc tggcgaacac gccttcgaag
     2821 cgcggcgtga tcgtggcgat gaacccgacg aacggcgaaa tcctgtcgct cgcctcgtac
     2881 ccgaccttcg accccaacct cttctcgcag cgcatcggca cgcccgaagg gcgccgcgag
     2941 gccaacgcgc tcttgaggga cacgcgcaac ccgctctaca accgcgccat caaggggcgc
     3001 tacccgcccg gctcgacgtg gaagattccg atggcgatgt cggggttgga gcagggcgcg
     3061 atcacggtca agaactcgaa cctcgcgtgc ggcggcggca tcaccatcgg caacaagttc
     3121 acgcgctgca tgggcagcca cggcgcgccg cccgtccgct tcgccataca ggtctcgtgc
     3181 gacggctact tctaccgcct cggcctcaag atgaagctcg aaggcatcca gaagatggtt
     3241 gaagactggg agatcaacaa gaagacgggc gtcgatctgc cgcgcgagct ggtgagctgg
     3301 acgccctcgc gcgagttcaa gcgccgcttc aacaaggccg accccgagtg gcgcgacatc
     3361 gacacggtct acgcctcgtt cgggcaggtg cacgagttcg tcacgccgct ggcgatgctc
     3421 cgcgccgtcg caggcatcgg catgggcggg aagatttatg tcccgcacct gctgaaagag
     3481 gcacgggcgg tggcggcggt cggccccttc gcggcgcggc ccgcggtcgg cttcgcgcgc
     3541 cccgagccga agatcgtccc gctctcggtc gagaatcacc agatggtcat gcacgggatg
     3601 tcggacgtgg tcaacgcggg cggcacgggc gggcgcatca agatggaggg cttcgagatc
     3661 gcgggcaaga ccggcaccgc gcaggtcgtc tcgctcggca aggacgtggg cgccaacaaa
     3721 gaccacgcgt ggttcgtctc gttcgcgccc
//
LOCUS       DBNGOH010000666         8934 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_98312, whole genome shotgun sequence.
ACCESSION   DBNGOH010000666 DBNGOH010000000
VERSION     DBNGOH010000666.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8934)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8934)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8934
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_98312"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..777
                     /locus_tag="AC159P_12940"
     CDS             <1..777
                     /locus_tag="AC159P_12940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013226064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonidase"
                     /protein_id="HIR3696400.1"
                     /translation="VTGYDLGTFLDGTFKRTQELAPDRDRVTGRIKFGTESNGGFKNI
                     TISNCVFDRSRGLAIETVDGGLIEDITVTNITMRDVTTAPLFLRLGNRARGPNNPPPG
                     AIRRVEISNLTAYDADPRYASIIAGLPNHPIEDVRLSNIRIVYKGGGTKQDAAAEPEE
                     RESSYPEPSMFGPIPAYGFYVRHARGLVMRDVEVSFMKDEQRPAFFLDDVTDVVFDGA
                     RAKRTAGVPFFVLRNVTDFTVRNTNGLPDTRKEKAERETL"
     gene            879..2762
                     /locus_tag="AC159P_12945"
     CDS             879..2762
                     /locus_tag="AC159P_12945"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan lyase"
                     /protein_id="HIR3696401.1"
                     /translation="MLESGARTLLGVAFIVVALCSPEAVAQRQMEGLGRGVVAVRQAD
                     GKVFVGWRVLGTEPDEIAFNLYRATDGARAAKLNERPLVEASSFVDEKADASRSHAYF
                     VRPVLKGREQEPSAAFTLAANAPARQYLSVPLQTPQGYTPNDASPGDLDGDGEYELVL
                     HQAGRGRDNGRPGLTTEPLLEAYKLDGTLLWRINLGKNIREGAHYTQFMVYDLDGDGR
                     AEVACKTADGTTDGKGKVIGDAAADWRSTEAGTLGKIMRGPEYLTVFDGLTGAALATV
                     DYVPARGDVAAWGDATGNRVDRFLACVAYLDGKRPSLVMARGYYTRTVLAAWNWRGGK
                     LTHVWTFDSDAPGNKGYAGQGNHNLSVADVDGDGRDEIVYGQCAIDDDGRGLYTTGLG
                     HGDAMHLSDLDPARPGLEVFSIQERFDDAGAHMVDARTGQVLWKKPSVQAGEDGEGPG
                     RGLALDIDPRHRGFESWVFGAGIRGLFNAKGEKIFERQPRSCNFGVYWDGDTLSELLD
                     RNRVSKWNWTDGTETVLLTAEGCTSNNGTKATPALSADLLGDWREEVVWRTVDNKELR
                     IYTTTTRTRHRFYTFMHDPQYRLSIAWQNVAYNQPPHAGFFVGEGMAKPPRPSIVLTK
                     AGR"
     gene            2962..3852
                     /locus_tag="AC159P_12950"
     CDS             2962..3852
                     /locus_tag="AC159P_12950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009719528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HIR3696402.1"
                     /translation="MSGNGMRPRLSIFVLVLCVLVLQGCGGGGRREERAGMVFVEGGT
                     FVMGTEGDMPEEAPAHRVTVNSFWMDAREVTVAEFARFVEATGYRTEAERFGWSGVFN
                     AETGEWERVDGANWRRPDGPRSEAKADEPVCQVSWNDAAAYAKWAGKRLPTEAEWEYA
                     ARGGLAGKRYAWGDELRPGGKPVANWWQGRFPEGDTGEDGYAARRAPVGSFAPNGYGL
                     YDVGGNVWEWCADWFDAGYYARSPAANPAGPEAGEERVIRGGSWMCSENFCSNYRVSA
                     RSRATPDSGLNNLGFRCASD"
     gene            4046..5500
                     /locus_tag="AC159P_12955"
     CDS             4046..5500
                     /locus_tag="AC159P_12955"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013627887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004065 - arylsulfatase activity [Evidence
                     IEA];
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HIR3696403.1"
                     /translation="MKTTLERSVACALCLLLCLLACAPFVPPSAAQGARRKPNVIFIL
                     ADDLGYGDIGPYGQRRIRTPHLDRLAREGLRFTDFYAASPVCAPSRASLMTGQHQGRT
                     YIRGNSTKVPVPGQSGAAARRLPLRPRDVTVAEVLKGAGYATGVVGKWGLGEPGTTGV
                     PNAQGFDYFFGYLNQTHAHSHFPEYLWRNRERVELPRGTYSQDLFTEEALQFVRRERE
                     RPFFLYLAYIIPHANNELGRETGNGMEVPSDAPYTNERWPQQQKSYAAMVTRMDADIG
                     RLMSLLKELGLDDDTLVLFSSDNGPQGRDEGGFDPDFFQSHGPLRGIKRDLYEGGVRV
                     PLLARWPGRIKAGQVSRVAWANYDLLPTLAALAGARAPRDIDGVSMLSALLGRGQQRA
                     REHLYWEFHEGEFAQAVRLGRWKAVRKGFDKPTELYDLQSDVGETRDVAAVHPRVVRR
                     VEQIMKSERTESEFWPVEGGPAMLGSNAEARGNR"
     gene            5692..7077
                     /locus_tag="AC159P_12960"
     CDS             5692..7077
                     /locus_tag="AC159P_12960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013568126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HIR3696404.1"
                     /translation="MKYNVLFISSDDLRPELGAYGNRLIKTPNIDRLAARGLRFDRAY
                     AQYPLCNPSRTSLLTGRHPATTGVMDNNTYFRVKYPDWVTLPQHFKAHGYASLRSGKT
                     FHGGIDDQVSWTEGGEPTDPRITERGTNRRPAAPPRRDPEAETEPGAAPSPTQASRPQ
                     GNRSGNPQSDRIVVLEGDGESHGDYRTATRAIGYLEKYKDQPFFLSVGFVKPHSPPTA
                     PKKFFDLYDVEKMQLPPDFAERPSAPQGFPTLSVPTRNTDLFVGRDASPAEAREMMRA
                     YYASTSFMDAQVGRVLDALDRLGLRDKTIVVFWGDHGYHLGEKGKWSKAYSLFEIAAR
                     VPLVIAVPGARGRASARNVQLLDLYPTLAELCGLPAPQGVEGRSLAALLRKPDAAWNH
                     PAFTVTLYQGKLGKSVRTERWRYAEWEEGREGAMLFDHSRDPRELKNLAADPAHAKTV
                     REMKSLLTQLP"
     gene            7317..>8934
                     /locus_tag="AC159P_12965"
     CDS             7317..>8934
                     /locus_tag="AC159P_12965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HIR3696405.1"
                     /translation="MLKRIVRYGTFLTLLVVLVHAATPATSAQARVVTSFDADWRFLE
                     ADATGAEKPDFNDAAWRKLDVPHDWSIEGPFDKENPTGKDGGFLPSGVGWYRKSFTLP
                     AGYRGKRVFVEFDGVMANSDVWVNGHHLGKRPFGYISFRYELTPHLDFGAGRANVVAV
                     RADNSGQPASRWYTGAGIYRHVRLVVTDPVHVEHWGTFVTTPEVSAEHATVRVESTVA
                     NQSESPRSVSLEITLVGPDGRAGAGFETRPQAIAPGHSAVFRHDIALKNPRRWDIHQP
                     HLYQAVVKVRAGARTLDDDRVTFGIREFRFDAPTGFWLNGRNFKLLGVCLHHDASALG
                     AAVPLRAWERRLEILREFGVNAIRTAHNPPAPEFLDLTDRMGFIVMDETFDAWTVKKR
                     EHDYHRYFDEWSKIDTRDTVRRDRNHPSIVVWSTGNEIHDTPKAELAKRILSGLVEVF
                     HEHDPTRPVTQGLFRPNVSKDYDNGLADLLDVVGQNYRENEILAAHRQKPSRKILGTE
                     NTHTREAWLALRDNPPYAGQFLWSGIDYLGE"
BASE COUNT         1535 a         3029 c         3022 g         1348 t
ORIGIN      
        1 gtcacgggct acgacctcgg cacgttcctc gacggcacgt tcaagcgcac gcaggagctg
       61 gcgcccgacc gcgaccgcgt gacgggccgc atcaagttcg gaacggagtc caacggcggc
      121 ttcaagaaca tcaccatctc caactgcgtc ttcgaccgct cgcgcggcct cgccatcgag
      181 accgttgacg gcggcctcat cgaagacatc accgtcacca acatcaccat gcgcgacgtg
      241 acgaccgcgc cgctcttcct ccggctcggc aaccgcgcgc gcggccccaa caacccgccg
      301 cccggcgcca tccgccgcgt cgagatcagc aacctcacgg cctacgacgc agacccgcgc
      361 tacgcctcga tcatcgccgg cctgcccaat catccgattg aagacgtgcg cctctccaac
      421 atccgcatcg tctacaaggg cggcgggacg aagcaggacg ccgccgccga gccggaggag
      481 cgcgaaagca gctaccccga gccgagcatg ttcggcccca tcccggctta cggcttctac
      541 gtccggcacg cgcgcggact cgtcatgcgc gacgtggagg tgagcttcat gaaggacgag
      601 cagcgccccg ccttcttcct cgacgacgtg acggatgtcg tcttcgacgg cgcgcgcgcc
      661 aaacggacgg cgggtgtccc cttcttcgtc ctccgcaacg tcacggactt caccgtccgc
      721 aacaccaacg gcctccccga cacgcgcaaa gagaaggcgg agcgcgagac gctctgaacg
      781 gcgcgggggg aaaaggggaa aaggggaaaa ggcgaaaagg cgttttgtct tcccctcttc
      841 gccttttccc cttttcccct tcaagggata gagtgttaat gcttgaatcc ggcgcaagga
      901 cgttattggg cgtcgccttc atcgtcgtcg cgctgtgctc gcccgaagcg gtcgcgcagc
      961 ggcagatgga gggcttgggg cgcggcgtgg ttgcggtgcg gcaggcggac gggaaggtct
     1021 ttgtcgggtg gcgcgtgctc ggcacggagc cggacgagat cgccttcaac ctctaccgcg
     1081 cgaccgacgg cgcgcgggcc gcgaagctca acgagaggcc gcttgtcgaa gcgtcgagct
     1141 tcgttgacga gaaagccgac gcctcgcggt cgcacgcgta ctttgtccgg cccgtcctga
     1201 aggggagaga gcaggagccg agcgcggcgt tcacgctcgc cgcgaacgcg cccgcgcggc
     1261 agtacttgag cgtgccgctc cagaccccgc agggctacac gccgaacgac gcgtcgccgg
     1321 gcgacctcga cggcgacggc gagtacgagc tggtgctgca tcaggcgggc cgaggccgcg
     1381 acaacggcag gcccggcttg acgaccgagc cgctcctcga agcctacaag ctcgacggga
     1441 cgctgctgtg gcgcatcaac ctcggcaaga acatccgcga gggcgcgcac tacacgcagt
     1501 tcatggtcta cgatctggac ggcgacgggc gcgccgaggt cgcctgcaag acggcggacg
     1561 gcacgacgga cggcaagggc aaagtcatcg gcgacgccgc cgccgactgg cgcagcacgg
     1621 aggcgggcac gctcggcaaa atcatgcgcg ggccggaata tctcacggtc ttcgacgggc
     1681 tgaccggcgc ggcactcgcc accgtcgatt acgtcccagc acgcggcgac gtggccgctt
     1741 ggggcgacgc gaccggcaat cgtgttgacc gcttccttgc gtgcgtcgct tacctcgacg
     1801 gcaagcgccc gagcctcgtc atggcgcgcg gctactacac gcgcacggtg ctcgcggcat
     1861 ggaactggcg cggcggcaag ctgacgcacg tgtggacttt cgacagcgac gcgccgggca
     1921 acaagggcta tgcggggcag ggcaaccaca acctgagcgt ggcggacgtg gacggcgacg
     1981 ggagggacga gatcgtctac ggccagtgcg cgatcgacga cgacggcagg ggcctctaca
     2041 cgacgggcct cggccacggc gacgcgatgc acctctcgga cctcgacccc gcgcggcccg
     2101 gcctcgaagt cttctccatc caggagcgct tcgacgacgc gggcgcgcac atggtcgatg
     2161 cgcggacggg gcaggtgctt tggaagaagc cttcggtgca ggcgggcgag gacggcgagg
     2221 ggccgggccg cggcctcgcg ctcgacatcg acccgcgcca cagagggttc gagagctggg
     2281 tcttcggcgc ggggattcgc ggcctcttca acgcgaaggg cgaaaagata ttcgagcggc
     2341 agccgcgctc ttgtaacttc ggcgtctact gggacggcga cacgctctcg gagctgctgg
     2401 atcgcaaccg cgtctcgaag tggaactgga cggacggcac ggagaccgtg ctgctgaccg
     2461 ccgaaggctg cacgtcgaac aacgggacga aggcgacgcc cgctctgagc gccgacctcc
     2521 tcggcgactg gcgcgaggag gtcgtctggc gcaccgtcga taacaaggag ctgcgcatct
     2581 acacgaccac tacccggacg cgccaccgct tctacacgtt catgcacgac ccgcagtacc
     2641 ggctcagcat cgcgtggcag aacgtggcct acaaccagcc gccgcacgcg ggcttcttcg
     2701 tcggcgaagg gatggcgaag ccgccgcgcc cgtcaatcgt cttgacgaag gcgggcaggt
     2761 gagcgggttg attttcgaag cggagttgtt aattcggggt tgagtgtttg gggcgtcgat
     2821 tttcaacaca gagaacacgg agaagaaata gaagctttga aatcccgaat cttgaattcg
     2881 gttttctctg tgaactctgt gcgccctccg tgtcctctgt gttgaaatat agcgtgaccg
     2941 gcactcggct atgaatccgg catgagcggt aacggaatgc ggcctcgtct ctcgatcttc
     3001 gttctcgtcc tgtgcgtgct cgtgctgcaa ggctgcggcg ggggcgggcg gcgcgaggag
     3061 cgcgcaggga tggtgttcgt cgagggcggc acgttcgtga tgggcacgga gggcgacatg
     3121 cccgaagagg cgccggctca tcgtgtgacg gtcaattctt tctggatgga cgcgcgcgaa
     3181 gtgaccgtcg cggagttcgc gcggttcgtc gaggcgacgg gctaccggac ggaggccgag
     3241 cggttcggct ggtcgggcgt cttcaacgcg gagacgggcg agtgggagcg cgttgacggc
     3301 gcgaactggc gccgccccga cggcccgcgg tcggaggcga aggcggacga gccggtgtgt
     3361 caggtctctt ggaacgacgc ggcggcttac gcgaagtggg cgggcaagcg tctgccgacc
     3421 gaggccgagt gggagtacgc ggcgcgcggc gggctggcgg ggaagcgtta cgcgtggggc
     3481 gacgagctgc ggcccggcgg gaagccggtc gccaactggt ggcagggccg cttccccgaa
     3541 ggtgacacgg gcgaggacgg ctacgccgcg aggcgcgcgc ccgtggggag cttcgcgccg
     3601 aacggctacg ggctgtacga cgtgggcggc aacgtgtggg agtggtgcgc ggactggttc
     3661 gacgcggggt attacgcgcg cagtcccgcc gcgaaccccg ccgggccgga ggcgggcgaa
     3721 gagcgcgtga tccgcggcgg ctcgtggatg tgcagcgaga acttttgctc gaactaccgc
     3781 gtctcggcgc gcagccgcgc gacgcccgac agcgggctga acaatctcgg cttccgctgc
     3841 gcgagcgatt gacggctcgc cgcgcgggcg atgaatgaat ttagagaccg tcgccacact
     3901 gtaggggcag ggcaagtccg cggcccgcgg cttcgataca ttcaacattg aatcaattgg
     3961 ctgttgccgg gccgcggact tgccctgccc ctacaagcat catcaacgtt gaggtctggc
     4021 aacggcctct gagaagcttc gacgtatgaa gacgactctc gaaaggtctg tggcgtgcgc
     4081 gctttgcctc ttgttgtgtc tgctcgcgtg cgcgccgttc gtgccgccga gcgcggcgca
     4141 gggcgcgcgg cggaagccga acgtcatctt catcctcgcg gacgatctgg gctacggcga
     4201 catcggcccg tacgggcagc ggcgcatcag gacgccgcac ctcgaccgtc tggcgcgcga
     4261 ggggcttcgg ttcacggact tctacgcggc cagcccggtc tgcgcgcctt cgcgcgcgag
     4321 cctgatgacg gggcagcatc agggccgcac gtacatccgc ggcaactcga cgaaggtgcc
     4381 ggtgccggga cagagcgggg cggcggcgcg gcggctgccc ctgcgaccgc gcgacgtgac
     4441 ggtggccgag gtgctcaaag gcgcgggcta cgcgaccggc gtcgtcggca agtggggcct
     4501 cggcgagccg ggcacgaccg gcgtcccgaa cgctcagggc ttcgactact tcttcggcta
     4561 cctgaaccag acgcacgcgc acagccactt ccccgaatac ctctggcgca accgcgagcg
     4621 cgtcgagctg ccccgaggca cttactcgca agacctcttc acagaagagg cgctccaatt
     4681 tgtccgccgc gagcgcgagc gcccgttctt cctctacctc gcctacatca tcccgcacgc
     4741 gaacaacgag ctgggccgcg agaccggcaa cggcatggag gtgccttcgg acgcgcccta
     4801 cacgaacgag cgttggccgc agcagcagaa gagctacgcg gcgatggtga cgcggatgga
     4861 cgcggacatc ggcagactca tgtcgctcct caaggaactc ggcctcgacg acgacaccct
     4921 cgtcctcttc agcagcgaca acggcccgca ggggcgcgac gagggcggct tcgaccccga
     4981 cttcttccag agccacggcc ccttgcgcgg catcaagcgc gacctctacg aggggggcgt
     5041 gcgcgtcccg ctgctcgcgc gctggccggg acgcatcaag gccggccagg tcagccgcgt
     5101 ggcgtgggcc aactacgatt tgctgccgac gttggcggcg ctcgcgggcg cccgcgcgcc
     5161 gcgcgacatc gacggcgtct cgatgctgtc ggcgctgctc ggtcgcgggc agcagagagc
     5221 gcgcgaacat ctctactggg agttccacga aggcgagttc gcgcaggccg tgcggctcgg
     5281 acgctggaag gccgtgcgca agggcttcga caagcctacg gagctttacg acctacagag
     5341 tgatgtcggc gagacgcgcg atgtcgccgc cgttcatcca cgcgtcgtgc ggcgcgtcga
     5401 acagatcatg aagagcgagc ggacggagtc cgagttctgg cccgtcgagg gcgggccggc
     5461 catgctcggc tcgaacgcgg aggcgagggg gaatagatga cgagagcgtg cgtcctatcg
     5521 aacgttgacg gagtccggcc agcggctcct tcatccacga atcgccggtc gaggggttcg
     5581 cggcggcgcg gcctaagctt cgccttcgcc gcgtgcgtcg tcgcgttgct gtcttgcgcc
     5641 gtccttcggt tgcccgcggc ttcaggcgcg gcgccgccgc agagggcggc gatgaagtac
     5701 aacgtcctct tcatctcctc ggacgacctc agaccggaac tcggcgccta cggcaaccgc
     5761 cttatcaaga cgccgaacat cgaccgcctc gccgcgcgtg ggctgcgctt cgaccgcgcc
     5821 tacgcccagt accctttgtg caacccctcg cgcacgtcgc tgctgacggg ccgccaccct
     5881 gcgacgaccg gggtgatgga caacaacacc tacttccgcg tgaagtaccc ggactgggtg
     5941 acgctgccgc agcacttcaa ggcgcacggc tacgccagcc tgcgctcggg caagaccttc
     6001 cacggcggca tcgacgatca ggtctcctgg acggaaggcg gcgagccgac cgatccgcgc
     6061 atcaccgagc gcggcacgaa ccgccgcccc gccgccccgc cgcgacgcga ccccgaggcc
     6121 gagaccgaac cgggcgccgc gccctcgccg acgcaggcgt cgcgaccgca gggcaaccgc
     6181 tccggcaacc cgcagtccga ccgcatcgtc gtgctcgaag gcgacggcga gtcgcacggc
     6241 gactaccgga cggccacgcg cgccatcggc tatctcgaaa agtacaaaga ccagccgttc
     6301 tttctctccg tcggcttcgt caagccgcac agcccgccga ccgcgccgaa gaagttcttc
     6361 gacctctacg acgtggagaa gatgcaactg ccgcccgact tcgccgaacg cccgtcggcc
     6421 ccgcagggct tcccgactct ttcagtcccg acgcgcaaca ccgacctctt cgtcgggcgc
     6481 gacgcttcgc ccgcggaggc gcgcgagatg atgcgcgcct actacgcctc gacgagcttc
     6541 atggatgcgc aggtggggcg cgtgctcgac gcgctcgacc gtctgggctt gcgcgacaag
     6601 accatcgtcg tcttctgggg cgaccacggc taccacctcg gcgagaaggg caagtggtcg
     6661 aaggcgtatt cgctcttcga gatcgcggcg cgcgtgccgc tcgtcatcgc cgtgcccggc
     6721 gccagggggc gcgcgagcgc ccgcaacgtc caactgctcg acctctaccc gacgctggcg
     6781 gaactgtgcg ggctgcccgc gccgcaaggc gtcgaggggc gcagcctcgc cgcgctcctg
     6841 cgcaaaccgg acgcggcgtg gaaccacccc gccttcaccg tgacgctcta tcagggcaag
     6901 ctcggcaagt cggtgcgcac cgaacgctgg cgctacgccg aatgggagga gggccgcgag
     6961 ggcgcgatgc tcttcgacca ctcgcgagac ccgcgcgagc tgaagaactt ggccgcagac
     7021 cccgcccacg cgaagaccgt gcgcgagatg aagagtttgc tgacgcaact gccttgagga
     7081 actgcggctc tgccgcatga tgtgtggcga tgtggcggtt ccgccgcctg atgtgcggtg
     7141 aggcttcgcc tcaccggcga cgtgcttctt tcttagctga ggctacgcct cagccaaggg
     7201 cgtagcccct ggataatttt aaggcggccc tgaccggaaa ggcagagcct ttccgcacat
     7261 cgggcggcgg agccgcgacc gaaggcgcgg tgcgacgaaa atcaatcatg ggaaacatgc
     7321 tgaaaagaat cgtcaggtac ggaacgttct tgactctgct cgtcgtctta gtgcacgccg
     7381 cgacgcccgc gacttcggca caggcgcgcg tcgtcacgtc gttcgacgcg gactggcgtt
     7441 ttcttgaggc cgacgcgacg ggcgccgaga agccggactt caatgacgcg gcgtggcgta
     7501 aactcgacgt gccgcacgat tggagcatcg aggggccgtt cgacaaggag aacccgacag
     7561 gcaaggacgg cggctttctc ccgtcgggcg tcggctggta tcgcaagagt ttcacgctgc
     7621 ccgccggcta cagggggaag cgcgtcttcg tcgagttcga cggcgtgatg gcgaacagcg
     7681 acgtgtgggt caacggtcat catctgggca agcgaccgtt cggctacatc agcttccgtt
     7741 acgagctgac cccgcacctc gacttcggcg ccggtcgggc gaacgtcgtc gccgtgcgcg
     7801 cggacaactc gggccagccc gcttcgcgct ggtacaccgg cgccgggatt tatcgtcacg
     7861 tccgcctcgt cgtcacggac cccgtccacg tcgagcactg gggcacgttc gtcaccacgc
     7921 ccgaagtctc cgccgagcac gcgaccgtca gggtcgagag caccgtcgcc aatcaatcgg
     7981 agtcgccgcg cagcgtctcg ctcgaaatca cgctcgtcgg ccccgacggt cgcgcgggcg
     8041 cgggcttcga gacccgaccg caggccatcg cccccggcca ctccgctgtc ttccggcatg
     8101 acatcgccct caagaacccg cggcgctggg acatccatca gccgcacctc tatcaagcgg
     8161 tggtgaaggt tcgcgcgggc gcgcgcaccc tcgacgacga ccgcgtcacg ttcggcatcc
     8221 gcgagttccg cttcgacgcg ccgaccggct tctggctcaa cggtcgcaac ttcaagctgt
     8281 tgggtgtgtg tctgcaccac gacgcgagcg cgctcggcgc cgccgtcccc ttgcgcgcgt
     8341 gggagcggcg gctcgaaatc ctgcgcgagt tcggcgtcaa cgccatccgc accgcgcaca
     8401 acccgcccgc gcccgagttc ctcgacctca ccgaccgcat gggcttcatc gtcatggacg
     8461 agaccttcga cgcgtggacg gtgaagaagc gcgagcacga ctaccaccgc tacttcgacg
     8521 agtggtcgaa gatcgacacg cgcgacaccg tgcgccgcga ccggaatcat ccgtccatcg
     8581 tcgtctggag cacgggcaac gagattcacg acacgccgaa ggccgagctg gccaagcgca
     8641 tcctctcggg cctcgtcgaa gtcttccacg agcacgaccc gacgcgcccc gtcacgcagg
     8701 gactcttccg ccccaacgtc agcaaggact acgacaacgg actcgcagac ttgctcgacg
     8761 tggtcgggca gaactaccgc gagaacgaaa tcctcgccgc gcaccggcag aagccttcgc
     8821 gcaaaatcct cggcaccgag aacactcaca cgcgcgaggc ttggctggcg ctccgcgaca
     8881 acccgcccta cgcgggacag tttttgtgga gcggcatcga ctatctcggc gagt
//
LOCUS       DBNGOH010000667         2769 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_5047749, whole genome shotgun sequence.
ACCESSION   DBNGOH010000667 DBNGOH010000000
VERSION     DBNGOH010000667.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2769)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2769)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2769
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_5047749"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..346)
                     /locus_tag="AC159P_12970"
     CDS             complement(<1..346)
                     /locus_tag="AC159P_12970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696406.1"
                     /translation="MRRKLVASLLSLTILAPAWLGFAPAASAWVQRRRPAVPRTATAP
                     ARNSDARVARIADEYLRGHYAFNPTEATSAGLHEFDAQLETRSADAVAREARRLRGAL
                     ADLGRVAEWRLSP"
     gene            complement(569..1273)
                     /locus_tag="AC159P_12975"
     CDS             complement(569..1273)
                     /locus_tag="AC159P_12975"
                     /inference="COORDINATES: protein motif:HMM:NF021221.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnetosome magnetite crystal formation protein
                     family"
                     /protein_id="HIR3696407.1"
                     /translation="MSEKRSKYDTDPLDPDFVRRTEEVMERTRSLGREPQPEPARREP
                     ATAEEPTRRFDEQFANSYPSVFVPPVYQPPAHGQGYTSFGADAQQQQPPTAAAPGPSS
                     FYDPAGARPMQRPVQKLGIAENIACVLPYAPFYIGLIAAIVELLIVPRNETRTRFHAA
                     QGLALQLVLIAGTLAFKLIGSITDSGFGGWLFWMASFVFLIVSMFRVWEGKPHHLAPL
                     DDATRRLNEKFEPQVK"
     gene            complement(1322..1750)
                     /locus_tag="AC159P_12980"
     CDS             complement(1322..1750)
                     /locus_tag="AC159P_12980"
                     /inference="COORDINATES: protein motif:HMM:NF025819.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008235 - metalloexopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HIR3696408.1"
                     /translation="MDSPHITVRRAHVEEMIAHARECAPEECCGLVGGRGVAASSVYR
                     LRNVARDAHTAYEAAPEELFAAQREMRARGEELVGIYHSHPRAHEPAPSAADVRLAFY
                     PSAVYFIIGFAHEGEGVLRAFRISEGERSWEPVRWEAPAE"
     gene            complement(1851..>2769)
                     /locus_tag="AC159P_12985"
     CDS             complement(1851..>2769)
                     /locus_tag="AC159P_12985"
                     /inference="COORDINATES: protein motif:HMM:NF024785.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HIR3696409.1"
                     /translation="NHREVLAGVLDAAGVAAQMHGEALVALDKLDKIGREGVAREFAG
                     RGIVAESGEELLGFFENLPALEHAAEFVFDGGDGAQQTSKAEAYNRAALGRLVEFIGD
                     HERGAHGVEDLRRIIEYAAAHGTLDRIKIDPSLARGLSYYTGAIMEISVPDLAGSLGG
                     GGRYDNLVGMFSGRDVPACGFSLGLERIVVVMTEREMFPPELVDAPADVLVTLWNEEG
                     ARDALALASELRRAGLRVDVYPEADKLGKQFKYASSRGVRVVAVVGDEELKRGEVTLK
                     DMTSGEQQTVSRESVAATAREALSRRAAK"
BASE COUNT          428 a          940 c          956 g          445 t
ORIGIN      
        1 cgggcgacag ccgccactcc gcgacgcgtc cgaggtcggc cagcgcgccg cgcaaacgcc
       61 gcgcctcgcg cgccaccgcg tcggcgctgc gcgtttcgag ctgcgcgtcg aactcgtgca
      121 gccccgcgct cgtggcctcg gtcgggttga aggcgtagtg gccgcgcagg tactcgtccg
      181 cgatccgtgc gacgcgcgcg tccgagttgc gagcgggggc tgtggctgtg cggggcacgg
      241 cggggcggcg acgttgcacc cacgccgacg ccgcgggcgc gaagccgagc cacgcgggcg
      301 cgagaatcgt cagcgaaagg agcgaagcca cgagcttcct tctcatgcgg cgagccatcc
      361 ccggatgaat cttggtgaac gcgagtcagt ctgtgaaggg agacgaaccg cctgcgagat
      421 taacataaag aagtaggcag caggcagtag gcagtcacaa cataaagacg cagaggcttg
      481 ggactttacc tcccaagcct ctgcgccctg cttcgatatt ctgacctctg acttctgact
      541 tctgtcttga ctgcctactg cctaccgcct acttcacctg cggttcgaat ttctcgttga
      601 gccggcgcgt ggcgtcgtcg agcggggcga ggtggtgggg cttgccttcc cagacgcgga
      661 acatggagac gatgaggaag acgaaggagg ccatccagaa gagccagccg ccgaagccgc
      721 tgtcggtgat cgacccgatg agcttgaagg cgagggtacc ggcgatgagc acgagttgca
      781 gcgccagccc ctgcgcggcg tggaagcgcg tgcgcgtctc gttgcgcggg acgatcagga
      841 gttcgacgat ggccgcgatc agcccgatgt agaagggcgc gtaggggagg acgcaggcga
      901 tgttctccgc gatcccgagc ttctgaaccg ggcgctgcat cgggcgcgcg cccgcagggt
      961 cgtagaagga ggaggggccg ggcgccgctg ccgtcggagg ttgctgctgc tgcgcgtcgg
     1021 ctccgaaact cgtgtagccc tgcccgtgcg cgggcggctg gtagacgggc gggacgaaga
     1081 ccgaagggta cgagttggcg aactgctcgt cgaagcggcg cgtcggctct tccgccgtcg
     1141 cgggttcgcg gcgcgcaggc tccggctgcg gctcgcgccc gagcgaccgc gtccgctcca
     1201 tcacctcctc ggtgcggcgc acgaagtcgg ggtcgagcgg gtctgtgtcg tacttgctcc
     1261 tcttctcaga catacaagcc ttcttacgaa acgggcggct tgcgggtttc ggcggcgcgg
     1321 ctcactcggc gggcgcctcc cagcggacag gctcccagct ccgctccccc tccgagatgc
     1381 ggaatgcacg cagcacgcct tcgccttcgt gtgcgaagcc gattatgaag taaacggcgg
     1441 aaggataaaa ggcgagtcgc acgtccgcgg cggaaggcgc gggttcgtgc gcgcgcgggt
     1501 gcgagtggta aatcccgacc agctcctcgc cgcgcgcgcg catctccctc tgcgcggcga
     1561 acaactcttc gggcgcggcc tcgtaagccg tgtgtgcgtc gcgcgcgacg ttacgcaggc
     1621 ggtagacgct cgacgccgcg acgccgcgcc cgccgacgag gccgcagcac tcctcgggcg
     1681 cgcactcgcg cgcgtgcgca atcatctcct cgacgtgcgc gcggcggacg gtgatgtgcg
     1741 gcgagtccac ggaagtttca ggaagaaggt taaaagggga aaagggggaa aggcgaaaag
     1801 gtgttttgtt ttccccctct tcgccttttc cccttttccc ttttaacctt tcatttggct
     1861 gcccgccgcg acaaagcctc gcgggcggtc gcggcgaccg actcgcggct caccgtctgc
     1921 tgctcgccgc tcgtcatgtc cttgagcgtc acctcgccgc gcttcagctc ctcatcgccg
     1981 acgacggcga ctacccggac gccgcgcgac gaggcgtact tgaactgctt gccgagcttg
     2041 tcggcctccg ggtagacatc gacgcgcagc cccgcgcgcc ttagctccga ggcgagcgcg
     2101 agcgcgtcgc gcgcgccctc ctcgttccag agcgtcacca gcacgtcggc gggcgcgtca
     2161 accagctcgg gcgggaacat ctcgcgctcg gtcatcacga cgacgatgcg ttcgaggccg
     2221 agcgagaagc cgcaggccgg cacgtcacgc cccgagaaca tcccgacgag gttgtcgtag
     2281 cgcccgccgc cgccgaggct tccggcgagg tcgggcacgc tgatctccat gatcgcgccc
     2341 gtgtagtagg acagcccgcg cgcgagcgag gggtcgatct tgatgcgatc cagcgtgccg
     2401 tgggcggcgg cgtattcgat gatgcggcgc aagtcttcga cgccgtgcgc gccccgctcg
     2461 tggtcgccga tgaactcgac gaggcgaccg agcgcggcgc ggttgtacgc ctccgccttc
     2521 gaagtctgct gcgcgccgtc gccgccgtcg aagacgaact ccgccgcgtg ctcaagcgcc
     2581 ggaaggttct cgaagaagcc gagcagctcc tcgccgctct cggccacgat gccgcgcccg
     2641 gcgaactccc gcgccacacc ctcgcgcccg atcttgtcca gcttgtccag cgcgaccagc
     2701 gcctcgccgt gcatctgcgc ggcgacgccc gccgcgtcga gcacgcccgc gagcacctcg
     2761 cggtggttg
//
LOCUS       DBNGOH010000668         2951 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_647719, whole genome shotgun sequence.
ACCESSION   DBNGOH010000668 DBNGOH010000000
VERSION     DBNGOH010000668.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2951)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2951)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2951
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_647719"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..761)
                     /locus_tag="AC159P_12990"
     CDS             complement(<1..761)
                     /locus_tag="AC159P_12990"
                     /inference="COORDINATES: protein motif:HMM:NF015380.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clostripain-related cysteine peptidase"
                     /protein_id="HIR3696410.1"
                     /translation="MSTKKQTRPPTNGCPKPPLKKAAPRHKWTVMIFLSGDNNLSEEC
                     VYALTEMMKAEMMKDGSNKEVAVVAQLDTGIVQDQRIFINNLIEKKKNGPGSLIAFVG
                     PQRQERRRRLKAALAARKQAAARVAQQNGHAGKRVPGAKQSPPEKRPVGHFDVLLDFV
                     TESVRAYPAEHYMLVLSGHGSGTVGDFLTKQEAKRVGTLSIPSLGGLLETIRVCVLDG
                     KKLDILGMDSCLMSMIEVAYAVHNNVEVMIGAEGFA"
     gene            complement(1216..1362)
                     /locus_tag="AC159P_12995"
                     /pseudo
     CDS             complement(1216..1362)
                     /locus_tag="AC159P_12995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014101405.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type sigma factor"
     gene            1472..1966
                     /locus_tag="AC159P_13000"
     CDS             1472..1966
                     /locus_tag="AC159P_13000"
                     /inference="COORDINATES: protein motif:HMM:TIGR02246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgcJ/EcaC family oxidoreductase"
                     /protein_id="HIR3696411.1"
                     /translation="MNTHTRSVRIITLVTALAIALGASALAATARATDSSDEAAIRDN
                     VRQLETGWNRKSGALFAKPFAEDADYVVINGMHIRGREAIDKGHQHIFDTIYKETTLS
                     LSVERIRHLRPDVALVHVHARATTPHPEGPREKEGRLTLVMSKEKEGWKIAAFQNTEV
                     VKNQ"
     gene            complement(2105..>2951)
                     /locus_tag="AC159P_13005"
     CDS             complement(2105..>2951)
                     /locus_tag="AC159P_13005"
                     /inference="COORDINATES: protein motif:HMM:NF025086.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HIR3696412.1"
                     /translation="RTRVTRRRTLGVMLRVFVPFVLIASVMIPAAGQRRDPFDFTSPP
                     PPPRTRASSSPADGPGEFTFARLIYTSPYSPYNRGMGGSWTVDYPEADNHFITGIREW
                     AGTNLNVAPRPEALEISDERLFDFPFIYIVEPGFMELSDEEVPRLREYLMRGGFLFLD
                     DFWGEYEWQNVQAQFKKVLPEHEIKELPMTHPLLHSYLDIEEVIQVPNIYNAQRGITS
                     EKGGTTPYYMAIEDKNGRMMVFISRNSDLGDAWEWINDSRYPVKYGLAAYRIGINVVI
                     YAMSH"
BASE COUNT          486 a          962 c          977 g          526 t
ORIGIN      
        1 gcgaagccct cggcgccgat catcacctcg acgttgttgt ggacggcgta ggcgacctcg
       61 atcatgctca tcaggcaact gtccatgccg aggatgtcga gcttcttccc gtcgaggacg
      121 cacacgcgga tcgtttcgag cagaccgccg aggctgggga tgctcagggt gccgacgcgt
      181 ttcgcctcct gcttggtgag gaagtcgccg accgtgccgc tgccgtggcc gctgaggacg
      241 agcatgtagt gctcggcggg gtatgcccga accgactcgg tgacgaagtc gaggagcacg
      301 tcgaagtggc ccacgggccg cttctccggg ggcgactgct tggcgcccgg cactctcttc
      361 cccgcgtgac cgttctgctg cgccacgcgc gccgccgcct gcttccgcgc cgcgagcgcg
      421 gccttcagcc gccgccgtcg ctcctgccgc tgcgggccga cgaaggcgat gagcgagccg
      481 gggccgttct tcttcttctc gatgaggtta ttgatgaaga ttctttgatc ctggacgatg
      541 ccggtgtcga gctgagcgac gacggcgacc tccttgttcg agccgtcttt catcatctcc
      601 gctttcatca tctccgtgag ggcgtagacg cactcctcgc tgaggttgtt gtcgccgctg
      661 aggaagatca tcaccgtcca cttgtgccgc ggagcggcct tcttcaaggg gggcttgggg
      721 cacccgttcg tcgggggccg ggtctgtttt ttcgtgctca tcgccgagcg ctccttcgca
      781 gttatgagat ggggtggcgg cgggcgggcc gcgctccggg gcgggagccg cgagaccaaa
      841 ccgcaaggcg aaagcccggt cgtccggcga gaccgggacg tgtcccgccg accctgcgcg
      901 gcaagctgcc gtgcggatgg ggccgccttc tcccggggag ttgcttccct gttatggtat
      961 gacttggggt gcggaagtat ttcttacttc agtcacctcg gggcgaacga tgaccgcaag
     1021 ttgcatgctc tggaaggcga cggcgacggc ggttgctgcc gttctcgcgc cgggccactg
     1081 aaaggtgggc ggactctaaa ccatcgggtg cgggatggga agttttttcc gacgggcggc
     1141 gcggcggcgg caagacgaaa gccgcgcggc gcgtcagtcg tcgaggagcc tgttgccgag
     1201 gagcgggtgg ccggcgagcc tgaggtacgc ctcgttgacg agtgccgtcg cttgcagcgt
     1261 gtgccccgcg cgctcgccgc tcatctgcct gcgcgcgatg cggcgcagct cggcgcgggc
     1321 tacgctctcc tcgcgctcgc cctgaaggcg gtcgagcttc agagcctctc cgccctcgcg
     1381 cgcgacggtt tccgaagcgc cccggtggcg cgccgacggc gcttcgcccc gatcaagact
     1441 tccgacactt ccatcacaag gagaatgaac attgaacacg cacacacgca gcgtccgaat
     1501 catcaccctc gtcacggcgc tcgccatagc gctcggcgcc agcgcgctcg ccgcgaccgc
     1561 gcgcgcgacc gactcgtcgg acgaggcggc catccgcgac aacgtcaggc agttggagac
     1621 gggctggaac cggaagagcg gtgcgctctt cgccaagccc ttcgccgagg acgccgacta
     1681 cgtcgtcatc aacggcatgc acatccgcgg gcgcgaggcc atcgacaaag gccaccagca
     1741 catcttcgac accatctaca aagagaccac cctcagcctc tcggtcgagc ggattcgcca
     1801 cctccgtccc gacgtggccc tcgtccacgt ccacgcccgc gccacaacgc cgcaccccga
     1861 aggcccgcgc gaaaaggagg gccgcctcac cctcgtcatg tccaaagaga aagagggctg
     1921 gaagatcgcc gccttccaga acacggaggt ggtaaagaac caatagggga agaggggaaa
     1981 aggggaaaag gaagagaagt cgaaagggga agagggagga tgagaggcac gctgcttctc
     2041 atcctccctc ttcgcctctt aaccttttcg ccttttcccc tcttcccctt ttcgcccttc
     2101 ttccctagtg gctcatcgcg tagatgacga cgttgatgcc gatgcggtag gcggcgaggc
     2161 cgtacttgac ggggtagcgc gagtcgttga tccactccca cgcgtcgccg aggtcggagt
     2221 tgcgcgagat gaagaccatc atccgcccgt tcttgtcttc gatggccatg tagtagggcg
     2281 tcgtgccgcc cttctcggag gtgatgccgc gctgcgcgtt gtagatgttc ggcacctgta
     2341 tgacctcctc gatgtcgagg taggagtgga gcagcgggtg cgtcatgggc agctctttga
     2401 tctcgtgctc gggcagcacc ttcttgaact gcgcctgcac gttctgccac tcgtactcgc
     2461 cccagaagtc gtcgaggaag aggaagccgc cgcgcatcag gtactcgcgc aggcgcggca
     2521 cctcctcgtc cgagagttcc atgaagcccg gctcgacgat gtagatgaac gggaagtcga
     2581 acaggcgctc atccgagatt tcgagcgcct ctgggcgcgg cgcgacgttc aggttcgtcc
     2641 ccgcccactc gcggatgccc gtgatgaagt ggttgtcggc ctccgggtaa tcgaccgtcc
     2701 acgacccgcc catcccgcgg ttgtagggcg aatagggcga cgtgtagatg agccgcgcga
     2761 aggtgaactc gccggggccg tcggcgggcg acgacgaggc gcgcgtccgc ggcgggggcg
     2821 gcggcgaggt gaagtcgaac gggtcgcgcc gctgccccgc cgccggaatc ataaccgacg
     2881 cgatgagcac gaagggcacg aagaccctca acatcactcc caatgttcgc cgcctcgtca
     2941 cgcgcgtccg g
//
LOCUS       DBNGOH010000669         2543 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3398725, whole genome shotgun sequence.
ACCESSION   DBNGOH010000669 DBNGOH010000000
VERSION     DBNGOH010000669.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2543)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2543)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2543
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3398725"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..738)
                     /locus_tag="AC159P_13010"
     CDS             complement(<1..738)
                     /locus_tag="AC159P_13010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HIR3696413.1"
                     /translation="MTTAIEQTLETYGIEDWGAGYFGINRKGNLVVHPSETDQARAAD
                     VKEIIEDLRKRGISTPVLLRFPQLVTAQVRKLQRAFRNSIREYEYQGTHMCVYPMKVN
                     QNRSVVEEYLREGTRYDFGLEAGSKAELYGALAMEQSPDSLLVLNGFKDAEFIELAYT
                     GARAGKRVVIVIEKLNELDHVLRIAERYEEGQLPMVGIRVKLYSKGSGRWEKSGGEAA
                     KFGLTTTEILEVIRRLQEEGRIDMLRLL"
     gene            1153..>2543
                     /gene="glnA"
                     /locus_tag="AC159P_13015"
     CDS             1153..>2543
                     /gene="glnA"
                     /locus_tag="AC159P_13015"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012469935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:1901704 - L-glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HIR3696414.1"
                     /translation="MDAKTALKYAEDQEAKFVSVRFTDLVGAWHHLTFPIHELDEGSF
                     EDGFGFDASSLRGWASIHESDMLLVPDATRLWMDPFSEEDTLCLIANAVDPITKEGYG
                     MDPRSVAQRAENYLRFTGIADTVNFGPEAEFFIFDGVKVRNEQHAAGYEIHSDEGAWS
                     SDRGSDDFAGPNLGYRIRNKEGYVPVPPADSLSDLRSEISMTLERVGITVECHHHEVA
                     TAGQCEIDFRYSTLLGTADNLQLFKYTVRNVAHQQGKGATFMPKPLYGDNGSGMHCHQ
                     SLWKDEKPLFAGDGYAGLSDLARWYIGGLLKHAASIIAFAAPTTNSYKRLVPGFEAPV
                     NLAYSARNRSAAIRIPMFSTNPKLKRLEFRPPDPSCNPYLAFAAMLMAGLDGIQNRID
                     PGEPLDKDIYDLSPEELKAVPSLPGSLDDSLKALEADHDYLLKGDVFTPQLIDRWITY
                     KREKEIQPLRMRP"
BASE COUNT          463 a          862 c          770 g          448 t
ORIGIN      
        1 cagcaggcgc agcatgtcga tccgcccctc ctcctgcaag cggcggatga cttcgagaat
       61 ctcggtggtg gtgaggccga acttcgccgc ctcgccgccg gacttctccc agcgcccgga
      121 gcctttcgag tagagcttga cgcggatgcc gaccatcggg agctggccct cttcgtagcg
      181 ctcggcgatg cgcagcacgt ggtcgagttc gttcagcttc tcgatgacga tgacgacgcg
      241 cttgcccgcg cgcgcgccgg tgtacgccag ctcgatgaac tcggcgtcct tgaagccgtt
      301 cagcacgagc agcgagtcgg gcgactgctc catcgccagc gcgccgtaca actcggcctt
      361 cgagccggct tcgagtccga agtcgtagcg cgtgccctcg cgcaaatact cctcgacgac
      421 cgagcggttc tggttgacct tcatcgggta gacgcacatg tgcgtgccct ggtattcgta
      481 ctcgcggatc gagttgcgga aggcgcgctg gagtttgcgg acctgcgcgg tgacgagctg
      541 cgggaagcgc aggaggacgg gcgtcgagat gccgcgcttc cggaggtctt cgatgatctc
      601 cttcacgtcg gcggcgcgcg cctgatcggt ctccgaaggg tggacgacga ggttgccctt
      661 gcgattgatg ccgaagtacc cggcccccca gtcctctatg ccgtaggtct caagcgtctg
      721 ttcgatggcc gtggtcaact tctagctctc tcgcctcccc tctggttctc ttaaagtgtc
      781 gtggcaaact ttcgcgcggc agcctcctca agagtgcttg ctggttgaag attaagggat
      841 aggaaaacgt aatggcgcaa tgtaacagaa aaagggaagt cggtcaaaaa ctattttttt
      901 cgcgcgggcg cgggcttaac tggcgtgcgg caaaggttta gggcgcgacg ggacttgcgc
      961 gcgagggcgc gcccgcggac gcttccgacc gcggaacctt gacagacatg gggccgcggc
     1021 tataatgcct gctttttaca gcgtcgcccg atgcccaaag acgcacgccc tgtgataccc
     1081 ccgacgtcga gtcccgccgc agcccgaagg cggcaagcct ctgctacccc ctataatttt
     1141 ccaggagaca acatggacgc aaaaacggcc ttgaagtacg ccgaagacca ggaggcgaag
     1201 ttcgtctccg tccgtttcac agacctcgtg ggcgcctggc accacctgac ttttcccatc
     1261 cacgaactcg acgaaggctc gttcgaagac ggcttcggct tcgacgcttc gagcctcagg
     1321 ggttgggcct cgatccacga gtccgacatg ctgctcgtgc ccgacgcgac ccgcctctgg
     1381 atggacccgt tctccgaaga ggatacgctc tgcctcatcg ccaacgccgt cgatccgatc
     1441 accaaagagg gctacggcat ggacccgcgc tcggtcgcgc agcgcgccga gaattacctc
     1501 cgcttcaccg gcatcgccga caccgtcaac ttcggccccg aggccgagtt cttcatcttc
     1561 gacggcgtga aggtcaggaa cgagcagcac gccgccggtt acgagattca ctcggacgag
     1621 ggcgcgtgga gttccgaccg ggggtcggac gacttcgcag gccccaacct cggctaccgc
     1681 atccgcaaca aggagggcta cgtgcccgtc ccgcccgccg actccttgag cgacctgcgg
     1741 agcgaaatct cgatgacgct cgaacgcgtc ggcatcacgg tcgagtgtca ccaccacgaa
     1801 gtggccaccg ccggccagtg cgagatcgac ttccgctact cgaccctctt gggcaccgcc
     1861 gacaacctcc agctcttcaa atacacggtg cgcaacgtcg cgcaccagca gggcaagggc
     1921 gcgaccttca tgccgaagcc tttgtacggc gacaacggca gcgggatgca ctgccaccag
     1981 tctctgtgga aggacgagaa gcccctgttc gcgggcgacg gctacgccgg cctctccgat
     2041 ctggcccgct ggtacatcgg cggcctcctg aagcacgccg cctccatcat cgccttcgcg
     2101 gcgccgacga cgaacagcta caagcggctg gtgcccggct tcgaggcccc ggtcaacctc
     2161 gcctactcgg cgcgcaaccg ctcggcggcg atccgcatcc cgatgttctc gaccaacccg
     2221 aagctgaagc ggctggagtt ccgtccgccc gacccttcgt gcaacccgta cctcgcgttc
     2281 gcggcgatgc tcatggccgg actcgacggc atccagaacc ggatcgatcc gggcgagcct
     2341 ctggacaagg acatctacga cctctcgccc gaagagctga aggccgtgcc ctcgctcccc
     2401 ggctcgctcg acgactcgct gaaggcgctc gaagccgacc acgactacct gctcaagggc
     2461 gacgtgttca cgccgcagct catcgaccgg tggatcacct acaagcggga gaaggagata
     2521 cagcccttgc ggatgcgccc gca
//
LOCUS       DBNGOH010000670         1641 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_648319, whole genome shotgun sequence.
ACCESSION   DBNGOH010000670 DBNGOH010000000
VERSION     DBNGOH010000670.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1641)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1641)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1641
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_648319"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            200..790
                     /locus_tag="AC159P_13020"
     CDS             200..790
                     /locus_tag="AC159P_13020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696415.1"
                     /translation="MKLLARLVLCASLLAAFSAPALPQKKRKTACPVPPPSPFKHTGQ
                     IATSFDRSANGMRTTLEHPRAFNAAGAAIYLSASFTHQDPRRADTPSLELTFISVSPA
                     QKFREAHDLVLLCDGAQRSFAGATSYQTRGGSGSTLEAAKVQLSYDDVLAVTRARRVS
                     ARLGAAEFELTHNHLEALRELASLMAPSPSRWRTTE"
     gene            complement(912..>1641)
                     /locus_tag="AC159P_13025"
     CDS             complement(912..>1641)
                     /locus_tag="AC159P_13025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LolA-like protein"
                     /protein_id="HIR3696416.1"
                     /translation="VKSVVAAFAEAFRSNAADERRAGAAAEAGAAPVADARAAAVIEK
                     YVQAIGGRAAVEKISSRVSKGAFEGNFAGVQVGGTVEIFEKAPNKTVSLIAVSESGVT
                     RRGYTGKYGFEQLPFYGFRELEGAELADIRLNSELHWSIKLPSLYHKIEFAGQEKEGD
                     AELLILELTPRQGSTTRLYFDAKTGLLTRKDDVTFEDYREVEGVLMPFTYRTAVTSFK
                     LTEIEFNVAMDDDIFTEAKDCFTK"
BASE COUNT          263 a          610 c          480 g          288 t
ORIGIN      
        1 ccggacgcgc ggccctcgcc ccgcgccggc gccgcaagca agtcccatct cagcggttct
       61 ctacgtaatt caaaccaagc tttagcgacc cggactcgtg atgacgcgct gcctcgtcaa
      121 accgatctcc ccgagctttg ccgccccgcg cgacctcgct tcgtcaacgc gccgcgataa
      181 taaaattccg aggaacccca tgaagctgct cgcccggctc gtcctgtgcg cttcgctgct
      241 cgccgcgttc tcggcccccg ccctcccgca gaagaagcgg aagacggcgt gccccgtgcc
      301 gccgccctcg cccttcaagc acaccggcca gatcgccacc agcttcgaca ggtcggccaa
      361 cggcatgcgg acgacgctcg aacacccgcg cgccttcaac gcggcgggcg ccgccatcta
      421 cctctccgcc tctttcacgc accaggaccc gcggcgcgcc gacacgcctt cgctcgaact
      481 gaccttcatc tccgtctcgc ccgcgcagaa gttccgcgag gcgcacgacc tcgtcctgct
      541 ctgcgacggc gcgcagcgtt ccttcgccgg agcgacgagc taccagacgc gcggcggttc
      601 cggctccacg ctcgaagccg cgaaggtgca actctcctac gacgacgtgc tcgccgtcac
      661 gcgcgcccgc agagtctccg cgcgcctcgg cgccgccgag ttcgaactga cgcacaacca
      721 cctcgaagcc ctccgcgaac tcgccagcct catggccccc tcgccgagcc gctggcgcac
      781 gacggaatag caggcagcag gcagcgggca gtaaaaacag gagtcaggtg tcagaagaag
      841 gaagaggctt gggactttac ctcccaagcc tcttccttct tctgtcttga ctgcctgctg
      901 cctgctgccc gctatttcgt gaaacagtcc ttggcctctg tgaagatgtc gtcgtccatg
      961 gcgacgttga attcgatctc ggtgagcttg aaggaggtga cggcggtgcg gtaggtgaag
     1021 ggcatgagga cgccttcgac ctccctgtag tcctcgaagg tgacgtcgtc cttgcgggtc
     1081 agcaggccgg tcttggcgtc gaagtagagc cgggtcgtgc tcccttggcg cggggtcagt
     1141 tcgaggatga gcagttcggc gtcgccttcc ttctcctgcc ccgcgaattc gattttgtga
     1201 tagaggctcg gcagcttgat gctccagtgc agctccgaat tcaagcggat gtcggccagc
     1261 tcggcgcctt ccagctcgcg gaagccgtag aaaggcaact gctcgaagcc gtacttgccc
     1321 gtgtagccgc ggcgcgtgac gcccgactcg gagacggcga tgagcgagac ggtcttgttc
     1381 ggcgccttct cgaaaatctc gaccgtgccg cccacctgca cccctgcgaa gttcccttcg
     1441 aaggcgccct tcgagacgcg gctcgaaatc ttctcgaccg ccgccctgcc gccgatggcc
     1501 tgcacgtatt tttcgatcac cgcggcggcc cgcgcgtcag cgaccggcgc cgcgcccgcc
     1561 tccgccgccg cgcccgctcg ccgctcgtcc gccgcgttac tccggaacgc ctcggcgaac
     1621 gccgcgacga cgctcttgac c
//
LOCUS       DBNGOH010000671         3665 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2483215, whole genome shotgun sequence.
ACCESSION   DBNGOH010000671 DBNGOH010000000
VERSION     DBNGOH010000671.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3665)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3665)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3665
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2483215"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            17..1768
                     /gene="murJ"
                     /locus_tag="AC159P_13030"
     CDS             17..1768
                     /gene="murJ"
                     /locus_tag="AC159P_13030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013940155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HIR3696417.1"
                     /translation="MKKDTEDSTEQDAATTHEEAGVVAGETHVPVGAVTGEFPAAQTQ
                     SAPKPRTESTRGGAFLVGAGILLSRIVGLLRQRVFAYYLGTSDAMDAFQAAFRIPNFL
                     QNVFGEGALSASFIPVYAKLLANKEREEADRVAGAVFSILALVTSLVVLAGVFAAPVL
                     TDIIARGFEGEKRDLTVTLVRILFPGAGLLVLSAWCLGILNSHRRFFLSYSAPVIWNV
                     AIIAALVWAGTPARGAALSNLGVWGEVARYVAWGAVVGSALQFGVQLPTVFRLLEGLR
                     LSLAASNTNVRTVIRNFLPVFVSRGVVQISAFVDEMIATFLGTGAVAALNYTQSLYTL
                     PVSLFGMSVSAAELPAMSSAIGSRDEVAAQLRKRLDAGLRQISFFIVPSIVAMLTLGD
                     AMVGALYRTGNFSHGDVLYVWTILAGSAVGLLPTTLGRLYSSTFYALHDTRTPFRFAV
                     LHVVLALVLGYPAALYLPPALGVEMRWGAVGLTASAGVAGWIEFLLLRRRLNGRIGRT
                     GLPLSFAMKLWLSAALSAALAFALKLALGGPHPIILAALVLTPYGLLYFALTAAWGLP
                     EARQVINRFTRMLPGRR"
     gene            2072..2728
                     /locus_tag="AC159P_13035"
     CDS             2072..2728
                     /locus_tag="AC159P_13035"
                     /inference="COORDINATES: protein motif:HMM:NF019225.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HIR3696418.1"
                     /translation="MIRRLLLTTASAVMLMALSSVARADTVTVVGNATPTLATATVNC
                     SFNAQTNTFTFTITNTSLHDARITGIGFDLQPGGSGSGLNGFSGNVTFQPAGVDFAFS
                     DGNLGNVPQFGSAVLDFGFVTGSNFTGGDPNDGLPPGIAPNNQATFVVSGASFSGMTE
                     EQICNAIFVRFQRVGADGEGSDVGTPSDAHTPEPVTMLLLGTGLAGVAARVRRKRRKE
                     "
     gene            3088..>3665
                     /locus_tag="AC159P_13040"
     CDS             3088..>3665
                     /locus_tag="AC159P_13040"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HIR3696419.1"
                     /translation="MLVGSALTARADIIIATGNNPQPDENVLLNTGITGNPIFGTTNQ
                     TGFSVRFSSNENLTAPANGQARIEAADGTLTNLTIDIPGATFTSLILNIDAATAGSVS
                     FLVTEDNGMTTAGTFALGASGENFFTITAINGQRISSVNFTTTVAMVITNVDDVAQIR
                     IGGAQQQTGAVPEPTTMLLLGTGIAGIAAKMRR"
BASE COUNT          601 a         1315 c         1085 g          664 t
ORIGIN      
        1 cgtatcacgc cgcgagatga agaaggacac cgaagactcg accgagcagg atgcggcgac
       61 gacgcacgaa gaggcgggcg tcgtcgcggg agagacgcac gtccccgtcg gcgccgtcac
      121 gggtgagttc cccgccgctc aaacacagtc cgcgccgaag ccgcgcacag agagcacgcg
      181 cggcggcgcc ttcctcgtcg gcgcgggcat cctcctcagc cgcatcgtcg gcctgctccg
      241 ccagcgcgtc ttcgcctact acctcggcac ctccgacgcg atggacgcct tccaggcggc
      301 gttccgcatc cccaacttcc tccagaacgt tttcggcgag ggcgctcttt cggcgtcctt
      361 catccccgtc tacgcgaagc tgctggcgaa caaagagcgc gaagaggcag accgcgtcgc
      421 gggcgccgtc ttctccatcc tcgcgctcgt cacctctcta gtcgtgctgg ccggcgtctt
      481 cgccgcgccc gtgctcacgg acatcatcgc ccgcggcttc gaaggcgaga agcgcgacct
      541 cacagtcact ctcgtccgca tcctcttccc cggcgccggg ctgctcgtgc tctccgcctg
      601 gtgcctcggc atcctgaaca gccaccgccg cttcttcctc tcctactccg cgccggtcat
      661 ctggaacgtg gcgatcatcg ccgcgctcgt gtgggcgggc acgcccgccc gcggcgccgc
      721 actctcgaac ctcggcgtct ggggcgaggt cgcgcggtac gtggcgtggg gcgcggtggt
      781 cggcagcgcc ttgcagttcg gcgtccagct cccgaccgtc ttccggctgc tcgaaggctt
      841 gcgtctttcg ctcgccgcgt cgaacacgaa cgtccgcacc gttatccgca acttcctgcc
      901 cgtcttcgtc agccgcggcg tcgtgcagat cagcgccttc gtggacgaga tgatcgccac
      961 cttcctcggc acgggcgcgg tcgcggcgct caactacacg cagagcctct atacgctgcc
     1021 cgtcagtctc ttcggcatgt cggtctcggc ggcggagctg cccgcgatgt cgagcgccat
     1081 cggcagccgg gacgaggtcg ccgcccaact gcgcaaacgg ctcgacgccg gcctgcggca
     1141 aatctccttc ttcatcgtcc cttccatcgt cgccatgctc acgctcggcg acgcgatggt
     1201 cggcgccctc taccgcaccg gcaacttcag ccacggcgac gtgctctacg tctggacgat
     1261 cctcgccggc tcggcggtcg ggctgctgcc gaccacgctc gggcggctct actcctcgac
     1321 cttctacgcg ctgcacgaca cgcgcacccc cttccgcttc gccgtcctgc acgtcgtgct
     1381 cgccctcgtg ctcggctacc cggcggccct gtatcttccg cccgcgctcg gcgtcgagat
     1441 gcgctggggc gcggtcggcc tcacggcctc ggcgggcgtg gccggctgga tcgagttcct
     1501 gctgctgcgc cgccgcctca acgggcgcat cgggcggacg ggactgccgc tctctttcgc
     1561 catgaagctc tggctctcgg cggccctctc ggcggccctc gccttcgcgc tcaagctcgc
     1621 gctcggcggg ccgcacccga tcatcctcgc ggccctcgtc ctcacgccct acggcctgct
     1681 ctacttcgcg ctcaccgccg cctgggggct gcccgaggcg cgacaggtca tcaaccggtt
     1741 cacccgaatg ctgccgggcc gtcgctgacg acgcgcgaac gcaagaagcc gcgccacgaa
     1801 aacgacgcca ttacgaggac gatgcgggcg cgttcggtaa actcacggcg ctcttttccg
     1861 gaatcactcc gccgcatttc cattctggaa tcactccgcc attgccactg ttatttgcgg
     1921 acaagagttg aaaggaaact cttcatgagg gtcttacgac gagcgccctt ctggtctcgt
     1981 tgtctctcta cccgtttgcc ttccggcccc aagtcagatt ctggaaggcg cacccaaact
     2041 tggacctaca agggaaaagg aaaggcgcca gatgattcgt agacttcttc tcaccaccgc
     2101 ttccgccgtc atgctgatgg cactgagttc ggtcgcccgt gccgacaccg ttaccgtggt
     2161 cggcaacgcc acgccgacgc tggcgaccgc cactgtcaac tgttcgttca acgctcagac
     2221 caacaccttc accttcacca tcaccaacac ctcgctgcac gacgcccgga tcaccggcat
     2281 cggcttcgac ctccagcccg gcggcagcgg gtcgggcctg aacggcttca gcggcaatgt
     2341 caccttccag cccgcgggcg tcgatttcgc gttcagcgac ggcaacctcg gaaacgtccc
     2401 gcagttcggc tcggcggttc tggacttcgg cttcgtcacc ggcagcaact tcacgggcgg
     2461 ggacccgaac gacggcctgc cgccggggat cgcccccaac aatcaggcga cgttcgtggt
     2521 ctcgggcgcg tccttcagcg ggatgaccga ggagcagatc tgcaacgcca tcttcgtgcg
     2581 cttccagcgt gtgggcgcgg acggcgaggg cagcgacgtg ggcacgcctt cggatgctca
     2641 cacgcccgag ccggtgacga tgctgctgct cggcaccggc ctcgcgggag tcgcggccag
     2701 ggtgcggcgg aagagaagga aagaataacc cagccggagc ggatcatcgc gccgacgagc
     2761 cgggaaccgt ggctgaagcc gcgtttcccg gctcgttatt ttgtccggta acggctttac
     2821 aaattttcgg gaaggcttta aattcacttc cacttttctg aaaccgacgc agaattgata
     2881 gtttagtgaa tttgtcgtgc gcgagtgctt tgaaatccgc acgagctccc gcaattgccc
     2941 ctgcggatgc cacaggattc tgcttcggac tgacgcattt tcagtatcgg aaaagtgcta
     3001 cacaaggcac aacaagtagg taacaatgga ggatcactta tgcgcccctc gaaaaaactt
     3061 ctcttccctc tcttagtgtt tgcagtcatg cttgtcggca gcgccctgac ggcgcgcgcc
     3121 gacatcatca ttgcgacggg aaacaacccg cagcccgacg agaacgtgct gctcaacacc
     3181 ggtatcaccg gcaacccgat cttcggcacc accaaccaga cgggcttttc cgtccgcttc
     3241 tcctcgaacg aaaacctgac cgcgcccgcc aacgggcagg cgcgcatcga ggcggctgac
     3301 ggcacgttga ccaacctgac catcgacatc cccggcgcga ctttcacctc gttgatcctg
     3361 aacatcgacg cggcgactgc cggctcggtc tccttcctcg tcaccgagga taacgggatg
     3421 acgacggcgg gcacgttcgc cttgggggcg agcggcgaga acttcttcac catcacggcc
     3481 atcaacgggc agcggatcag cagcgtcaac ttcacgacga cggtggcgat ggtgatcacg
     3541 aacgtggacg acgtagcgca gatccgcatc ggcggggcgc agcagcagac cggcgccgtc
     3601 cccgagccga cgacgatgct tttgctcggc acaggcatcg cgggcatcgc tgccaagatg
     3661 cgtcg
//
LOCUS       DBNGOH010000672         3365 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4498102, whole genome shotgun sequence.
ACCESSION   DBNGOH010000672 DBNGOH010000000
VERSION     DBNGOH010000672.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3365)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3365)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3365
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4498102"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..348
                     /locus_tag="AC159P_13045"
     CDS             <1..348
                     /locus_tag="AC159P_13045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696420.1"
                     /translation="GKGEEEDPGPIIRDKFCVGEGDQRRCARYVFMDHEHEGFFNRAL
                     DSGWFEVAYEDDDCSILRLREEKGEPPPDNLPPGQQPSPPQDKDQQPDEETNPEEGAG
                     DEEGGAETESPQP"
     gene            367..954
                     /locus_tag="AC159P_13050"
     CDS             367..954
                     /locus_tag="AC159P_13050"
                     /inference="COORDINATES: protein motif:HMM:NF022167.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HIR3696421.1"
                     /translation="MKSHPPMPAPPSQVAALMRGFGPDWFVAGGWAIDLYLREETRPH
                     EDIEIAVFREDQRALHRHLDGWLLRKVVDGKLSVWHRDEWLARPVHEIQCSSETAEPR
                     RLEVLLNERSGDEWVFRRDERVRRPLAESCMVSDAGVRFLCPEVVLLYKSKGPRDKDE
                     HDFAAVVPRLDAERKRWLRAAIALNHPAHHWLQSL"
     gene            complement(968..1996)
                     /locus_tag="AC159P_13055"
     CDS             complement(968..1996)
                     /locus_tag="AC159P_13055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011421895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HIR3696422.1"
                     /translation="MKAVIFEEHGGPEVLRYEERPEPKVRANEVLVEVRACALNHLDV
                     WNRKGLPGVRIPLPHILGNDVAGVVAEAGEVADWVRPGDEVLLQPGVSCGHCRECLRG
                     ADNLCPEYDILGSRRDGGYAELVAVPAVNVVPKPKNLSWEEAAALPLVTLTAWHMLVT
                     RARVEPGEDVLVHAAGSGVGSIAIQVAKLRGARVIATAGSEEKLEKARALGADEVVNY
                     TNADWPKEVRHLTDKRGVDVVFEHTGAETWPGSILSLKRDGRLVTCGATSGYDARTDI
                     RQVFFRHLSLLGSMMGSKAELLDAMQFVERGAIRAVIDRTLPLAEARRAHELMEDRAQ
                     FGKLVLVP"
     gene            2241..3149
                     /locus_tag="AC159P_13060"
     CDS             2241..3149
                     /locus_tag="AC159P_13060"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3696423.1"
                     /translation="MRAEGASSTAYLIAGSTVFVARERIVGHLVPARSAELCARFIAA
                     RPRLAGRLLRSLAAGRLRRLGALVERLTVPGIQLHYALRKRFIEDEARAALAAGFTQA
                     VVLGAGFDTLAARLSGEFPRARFFEVDRHATQRLKTAALARDPTLGPNLFFLPLDLAC
                     GGLETALLSSPAFRRDQRTLFVAEGLLMYLAPTQVRALLRSLGASSARAPRFVFTFIE
                     PQQPGGRLAFRRAGRAVDAWLALRGEPFRWGATRAALAEQLAAEGFRTRAVADAAALR
                     RRYLDTASLARLPLAEGECICVAEAD"
BASE COUNT          536 a         1281 c         1047 g          501 t
ORIGIN      
        1 ggcaagggcg aggaggaaga ccccggcccc atcatccgcg acaagttctg cgtcggcgaa
       61 ggcgaccagc gccgctgcgc ccgctacgtc ttcatggatc acgagcacga aggcttcttc
      121 aaccgcgcgc tcgacagcgg ctggttcgaa gtcgcctacg aggacgacga ctgctccatc
      181 ctccggctgc gcgaagagaa gggcgagccg ccccccgaca acctcccgcc cggccaacag
      241 ccgtcgccac cgcaggacaa agaccaacag cccgacgaag agacgaaccc cgaagaaggc
      301 gcgggcgacg aagaaggcgg cgcggagacg gagagtccgc agccttgaac ggaagaaatc
      361 cttgccttga aatcgcaccc gccgatgccc gccccgccgt cacaggtcgc cgccctcatg
      421 cgcggcttcg ggccggactg gttcgtcgcg gggggctggg cgatcgacct ctacctccgc
      481 gaagagaccc gcccccacga agacatcgag atcgccgtct tccgcgaaga ccaacgcgcc
      541 ctgcaccgac acctcgacgg ctggctcctg cgaaaagtcg tggacggaaa gctctccgtc
      601 tggcaccgcg acgagtggct cgcgcggccc gtgcacgaga ttcaatgttc cagcgagacg
      661 gcggagccgc ggcggctcga agtccttttg aacgagagga gcggcgacga gtgggtgttc
      721 cgcagggacg aaagggtgag gaggccgttg gccgagtcat gcatggtctc ggacgcgggc
      781 gtgaggtttc tctgccccga ggtggtgctg ctctacaaat cgaaggggcc gcgcgacaag
      841 gacgagcacg actttgcggc ggtcgtcccg cgcctcgacg ccgaacgcaa gcgctggctg
      901 cgcgccgcca tcgccctcaa ccaccccgca caccactggc tacaaagtct ctgacccgca
      961 ccgcccttca aggcactaag acgagcttgc cgaactgcgc gcggtcttcc atcagctcgt
     1021 gcgcgcgccg cgcctcggcc agagggagcg tgcggtcgat gacggcgcga atggccccgc
     1081 gctcgacgaa ctgcatcgcg tcgagcaatt ccgccttcga acccatcatc gagccgagca
     1141 gcgagaggtg gcggaagaag acctgccgga tgtccgtgcg cgcgtcgtag ccgctcgtcg
     1201 cgccgcacgt cacgagccgc ccgtcgcgct tcagcgagag gatcgaaccc ggccacgtct
     1261 ccgcgcccgt gtgctcgaac acgacatcga cgccgcgctt gtcggtcagg tgcctgacct
     1321 ccttcggcca gtccgcgttc gtgtaattca ccacctcgtc cgcgcccaaa gcacgcgcct
     1381 tctcaagctt ctcctcggac ccggcggtgg cgatcacgcg cgcgcctctc agcttcgcca
     1441 cctggatggc gatcgagccg acgccgctgc ccgccgcgtg gacgagcacg tcctcgcccg
     1501 gctcgacgcg cgcgcgcgtc accagcatgt gccacgccgt cagcgtcacc agcggcagcg
     1561 ccgccgcctc ttcccagctc aaattcttcg gcttcggcac gacgttcacc gccggcaccg
     1621 cgaccagctc cgcgtaaccg ccgtcgcgcc gcgacccgag gatgtcgtac tccgggcaca
     1681 ggttgtcggc cccgcgcagg cactcgcggc agtggccgca gctcacgccc ggctgcaaca
     1741 gcacctcgtc gcccggcctc acccagtccg cgacctcgcc cgcctcggca acgacgcccg
     1801 ccacgtcgtt ccccagaatg tgcggcagcg gaatcctgac tcccggcagc cccttccggt
     1861 tccacacgtc cagatggttc agcgcgcacg cgcgcacctc caccagcacc tcgttcgccc
     1921 tgaccttcgg ctccggtcgc tcctcgtaac ggagcacctc cggcccgccg tgttcctcga
     1981 agatgacagc tttcatgaca gcggatttta gatttcggat cgcggattga aaagcgctca
     2041 ggcaagtgag cagtgagctg tgagcagtga gcagttaaaa gacggatgct caccgctcgt
     2101 ttgtagtttc gttcttactg cttactgctc accgctcact gcttactgtt caaggtgaca
     2161 atcgagaaag tgcaacagcg tgcgcggcgg ggttgccgtg cgcggacgta tgctccggcg
     2221 cgatgacgtt gcgaggagcc atgcgcgccg agggtgccag ttcgaccgcg tacctgatcg
     2281 cggggagcac ggtcttcgtc gcgcgcgagc gcatcgtcgg ccatctcgtg cccgcgcgct
     2341 cggcggagct gtgcgcgcgg ttcatcgccg cgcgcccgcg cctcgccggt cgcctgctgc
     2401 gctcgctcgc ggcggggcgc ctgcgccgcc tcggcgcgct cgtcgaacgc ctgacggtgc
     2461 ccggcatcca gctccactac gcgctgcgca aacgcttcat cgaagacgag gcgcgcgccg
     2521 cgctcgccgc gggcttcacg caagcggtcg tgctcggcgc gggcttcgac acgctggccg
     2581 cgcgcctctc cggcgagttc ccgcgcgcgc gcttcttcga agtagaccgc cacgccacgc
     2641 agcgactgaa gaccgccgcc ctcgcacgcg acccgaccct cggccccaac ctcttcttcc
     2701 tcccgctcga cctcgcatgc ggaggactcg aaacagcact cctctcatcc cccgccttcc
     2761 gccgcgacca gcggacgctc ttcgtcgccg aagggctgct catgtacctc gccccgacgc
     2821 aggtgcgcgc gctcctgcgt tcgctcggcg cctcctctgc gcgcgcgccg cgcttcgtct
     2881 tcaccttcat cgagccgcaa cagcccggcg gtcggctcgc cttccgccgc gcgggccgcg
     2941 ccgtggacgc gtggctcgcg ctgcgcggcg agcctttccg ctggggcgcg acccgcgccg
     3001 cgctcgccga acaactcgcg gcggagggct ttcgcacgcg cgccgtcgcc gacgccgcgg
     3061 ccctgcgccg ccgctatctc gacaccgcct cgctcgcgcg cctcccgctc gcggagggcg
     3121 agtgcatctg cgtcgccgag gccgactgaa aacttcggac attgaagaag gccggagtcg
     3181 gaaaccgaaa gagacgaaca tgagcgcgca caccacgcat ctcaagcacc gcgtccggcg
     3241 cctcgcgctg accctgcgcc accccgtccg ccgcgtgaag cgcggactgc ggacggcgcc
     3301 gccgccgctc ttcgtcaacg acatccactc gcaactgaac cgcacgcgcg tcgcgcgcct
     3361 cgccg
//
LOCUS       DBNGOH010000673         2490 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4498173, whole genome shotgun sequence.
ACCESSION   DBNGOH010000673 DBNGOH010000000
VERSION     DBNGOH010000673.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2490)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2490)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2490
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4498173"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(93..1037)
                     /locus_tag="AC159P_13065"
     CDS             complement(93..1037)
                     /locus_tag="AC159P_13065"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HIR3696424.1"
                     /translation="MPAGTRHQRTRPFIREMFRLFPSLLLILLLSLSAPARRQDDDDI
                     VRVESDLVILNVTVTDAQGRFIRKLRRTDFQVFEDGREQAINSFLVEETPFAAAILLD
                     TSSSMERRISLARAAAIRFLDGLRGDDVTAVYNFDNEVERMQDYSPSRDLPALAYDLK
                     TKGKTSLNDAIVLAAKDLSARPEKRRAIVVLSDGIDFGSRASSDKALNAALAAGASIY
                     AVNMIDKVAQPTLQMQAGAALKNFATKSGGRYVATPGGFELGEAFVGIAEELRNQYTL
                     SYRPANAARDGRWREIDLKVSRADASARTRKGYRAPKK"
     gene            complement(1048..1575)
                     /locus_tag="AC159P_13070"
     CDS             complement(1048..1575)
                     /locus_tag="AC159P_13070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696425.1"
                     /translation="MKSALFVLFMFVCAGTAQAQIRGDDSLDASNTPRNAPQQHPHPA
                     EEMRYRAAVRHEEETHREVIERAEEIDEISTRILKRYDSQKNLGPDDLKSLERIEKLA
                     RKIRGNAGGSDSEGSLDSPPSKLDAAVTRLSELSEQLNKGVKKTSRMVVSAAVIERSN
                     ELIRLVKHIKSFLRP"
     gene            complement(1760..2221)
                     /locus_tag="AC159P_13075"
     CDS             complement(1760..2221)
                     /locus_tag="AC159P_13075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696426.1"
                     /translation="MPIANVARTPEAVIYADDAMLRKPYAVLGGTVENISGSRLEEVS
                     VELELQKRSDGSRERRSVKVDPEDLGPGERGTYSLRILSDEWSDSRILSLRTQAGKNE
                     IAFRTAQGARRPPEVLPEKVTVTQTVNGPKPKKRPGDDEFINSPDNPIAVP"
BASE COUNT          435 a          748 c          814 g          493 t
ORIGIN      
        1 gtgcgctctg tgcgacccct gtgccctctc gtgttgaatc cgaagacccg caacactcaa
       61 ccgccgactg ccgacgcggg gccgcggtga ttctatttct tgggggcgcg gtagcctttg
      121 cgcgtccgcg cgctcgcgtc ggcgcgcgag actttcaggt ctatctcgcg ccagcgaccg
      181 tcgcgcgcgg cgttggcggg ccggtagctg agggtgtact ggttgcgcag ctcctccgcg
      241 attccgacga aggcttcgcc cagctcgaac ccgcccggcg tggcgacgta acggcccccg
      301 ctcttcgtgg cgaaattttt cagggccgcg cccgcctgca tttgcagcgt cggctgcgcg
      361 accttgtcga tcatgttgac ggcgtagatc gaggcgcccg cggcgagcgc ggcgttgagc
      421 gccttgtcgg aagaggcgcg actgccgaag tcgatcccgt cggagaggac gacgatggcg
      481 cgtctctttt cgggccgggc cgagaggtct ttggcggcca acacgatcgc gtcgttgagg
      541 ctcgtcttgc ccttcgtctt gaggtcgtag gcgagcgccg gcaggtctcg gctcggcgag
      601 tagtcctgca tccgctcgac ctcgttgtcg aagttgtaga cggcggtcac gtcgtcgccg
      661 cgcaggccgt ccagaaagcg gatcgcggcg gcgcgcgcga gcgagatgcg acgctccatg
      721 ctcgaagagg tgtcgagcag gatcgccgcc gcgaacggcg tctcctcgac gaggaaggag
      781 ttgatcgcct gctcgcgccc gtcctcgaag acttggaagt ccgtgcgtcg cagcttgcgg
      841 atgaaacggc cctgcgcgtc cgtcaccgtg acgttgagaa tcacgagatc ggactcgacg
      901 cggacgatat catcatcgtc ctgccgccgc gcgggggctg acagagagag cagaaggatg
      961 agcaggagtg aagggaagag gcggaacatc tcccgtataa acgggcgggt tcgttggtgg
     1021 cgcgttccgg caggcatctc gttcttgtca ggggcggagg aaagacttga tgtgcttgac
     1081 gagtcggatc aattcgttcg agcgctcgat gaccgccgcc gagacgacca tgcgtgaagt
     1141 cttcttcacg cccttgttga gctgctcgga caactcggac aggcgtgtga cagccgcgtc
     1201 gagcttggac ggcggactgt cgagcgaccc ttcggagtcc gagccgccgg cattgccgcg
     1261 aatcttgcgt gccagcttct cgatgcgctc cagactcttc aggtcgtcgg ggccgagatt
     1321 cttttgcgag tcgtagcgct tcagaatgcg tgtgctgatc tcgtcgatct cttcggcccg
     1381 ctcgatcacc tcgcggtggg tctcctcctc gtgacggacg gcggcgcggt agcgcatctc
     1441 ttcagccggg tgggggtgct gctgcggcgc gttccgcggt gtgttggacg cgtccagaga
     1501 gtcgtcacca cgaatctgcg cctgcgccgt cccggcacag acgaacatga atagtacgaa
     1561 cagggcgctc ttcaatcccg attctcctta aaacggccca ctccccggaa agactgctta
     1621 tgaaatcaac ccgttcgggc cgtgagacgg tgcgacggac ggaaaacgtt gtcttacggg
     1681 ctcagaaatt taagccggac gcctgaacat tctggtttaa gaaaagtagg cgggccgaca
     1741 tctaaaggat tacgttaaac tacggcaccg cgataggatt atccgggctg ttgatgaatt
     1801 catcgtcacc cggcctcttt ttcggcttcg gcccgttcac ggtctgcgtg acggtcactt
     1861 tctcgggcaa cacctcgggc gggcggcgcg cgccctgtgc ggtgcggaag gcgatctcgt
     1921 ttttgcccgc ctgcgtccgc aaagagagga tacgcgaatc gctccattcg tccgagagga
     1981 ttctgagtga gtaggtgccg cgctcgccgg ggccgaggtc ttcgggatcg accttgaccg
     2041 aacgcctctc acgactaccg tcggagcgtt tctgcaattc caactccacg gaaacctctt
     2101 cgagcctcga cccggagatg ttctccaccg tgccgccgag gacggcgtaa ggcttcctca
     2161 acatcgcgtc gtcggcgtag atcacggcct ccggggttcg cgcgacgttg gctatgggca
     2221 cattccgtcg cgcctgtgtg ggggcgggcg tggaagtgca gccggaaaaa agggatgtga
     2281 tggcgaccgt aacgatcaag cacagcgagg attcgaaacg catgattatt ccgaagcgac
     2341 tgaagacctc aacaaacgtc ttaagtgtgg gggcataagc tgccggtagc ggcgacttaa
     2401 ggtaaaaaca ttcccggcgc cgaaggtcac tgctgcggtc gtcccacagg aggcccaccg
     2461 gtcatccttt cgaccagcgc gatgatgaac
//
LOCUS       DBNGOH010000674         2523 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4498343, whole genome shotgun sequence.
ACCESSION   DBNGOH010000674 DBNGOH010000000
VERSION     DBNGOH010000674.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2523)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2523)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2523
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4498343"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..968)
                     /locus_tag="AC159P_13080"
     CDS             complement(<1..968)
                     /locus_tag="AC159P_13080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HIR3696427.1"
                     /translation="MPDILLVEDKESLRRVLRLTLENAGYTVAEAEDARAAAQEIARA
                     PFRLVLTDLRMPHGSGLDVLRAAKSADPEVPVVVMTAYGSIDEAVQAMKDGAHDFLQK
                     PVDSNHLLLLIERALEQARLRTENVLLREEWSRRYGFPRIIGESDAIKQAVAETQRVA
                     TTDTTVLLLGESGTGKELFARAVHHLSPRRDRAFVAINCAAIPETLIESELFGHERGA
                     FTGANERRPGKFELAAGGTIFLDEIGELPLAVQGKLLRAIEEKTVDRIGGRAPVPVDV
                     RIVAATNRDLEAAVADGQFREDLFFRLNVIPLNVPPLRERPSDIPLL"
     gene            complement(1207..2475)
                     /locus_tag="AC159P_13085"
     CDS             complement(1207..2475)
                     /locus_tag="AC159P_13085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696428.1"
                     /translation="MRERDCRLYRQRPRERGAALVMSLLISLLLLAAGGALLMATGMS
                     AANAADSTAEAQAYYAAEAGLQAALSTVRGNRPSSPAGTPATFRNLVCGTSDPCANTG
                     TLDLWLPRTNGVVPISNSPRLAYALSVQDAGLAEGAPVPPERDPRFLIITSRGFGPKG
                     ATKVLRMMVDAHPFEFTARAAVAIRSHDTDSTPMQLLDVGESDPHLWNGNDSAIPPGP
                     ALPAFAVTNTADFDAGDGFDKDSDQGSAEKAIGGDEANVLGDQQVVKLDPSGLEWWLQ
                     KAGSARIFVDIMKERATTLGRLNPGDIGTEASPKFTFIEGDLEIKGGDHGAGLLIVTG
                     KITQGGGSSFKGIILALGEGYIDRDGTPSLLGALIVAKFDRSPGATGDFLAPYVESSG
                     GGNSLVGYNSDWVRKALNSGGSRVVGIVEQ"
BASE COUNT          369 a          793 c          951 g          410 t
ORIGIN      
        1 agcaggggga tgtccgacgg ccgctcccgg agcgggggca cgttcagcgg gatcacgttc
       61 aggcggaaga acaggtcctc gcggaactgg ccgtcggcga ccgccgcctc gaggtcgcgg
      121 ttggtggcgg cgacgatgcg cacgtccacg gggacgggcg cccgcccgcc gatgcggtca
      181 acggtcttct cttcaatcgc gcgcaggagt ttgccctgca cggcgagggg cagctcgccg
      241 atctcgtcga ggaagatggt gccgcccgcg gccagctcga acttgccggg gcgccgctcg
      301 ttggcgccgg tgaaggcgcc gcgctcgtgg ccgaacagct cggactcgat gagcgtctcg
      361 gggatggcgg cgcagttgat ggcgacgaag gcgcggtcgc gccgcgggct gaggtggtgg
      421 acggcgcgcg cgaacaactc cttgcccgtg cccgactcgc cgagcagcag gacggtggtg
      481 tcggtcgtgg cgacgcgctg cgtctcggcg acggcctgtt tgatggcgtc ggactcgccg
      541 atgatgcgcg ggaagccgta gcggcgcgac cactcttcgc gcagcagcac gttctcggtg
      601 cgcagccgcg cctgttcgag cgcgcgctcg atgagcagga gcaggtggtt cgagtcaacc
      661 ggtttttgga ggaagtcgtg cgcgccgtcc ttcatcgcct gcacggcttc atcaatcgag
      721 ccgtaggcgg tcatgacgac gacgggcact tcggggtcgg cggacttcgc ggcgcgcagc
      781 acgtcgaggc cggagccgtg cggcatccgc aggtcggtga gcacgaggcg gaagggcgcg
      841 cgggcgatct cctgcgccgc ggcgcgcgcg tcttcggcct cggccacggt gtagccggcg
      901 ttctccagcg tcagccggag cacgcgccgc aggctctcct tgtcctcgac gagcaggatg
      961 tcaggcatga aagttcaatg atgaatgcgg aatgatgaac gatgaataga agacggcttg
     1021 tctttcattc atcgttcatc attccgcatt catcattccc ggtcgtggcg gatggtaacg
     1081 acgcgcgcgc gggcgtgtca agcaacggcc tcgcgcggac gaaggaggcg ggggcggaaa
     1141 caaagagggc gtggggaagc tgacgcacag cttgcccacg ccgctgcgtg ccgagggcgc
     1201 gcgcctctac tgctcgacga tgccgacgac gcgcgagccg ccggagttga gtgccttgcg
     1261 cacccaatcg gagttgtagc cgacgagcga gttgccgccg ccggaagatt cgacgtaagg
     1321 ggcgaggaag tcgcccgtgg cgccggggct gcggtcgaac ttcgccacga tcagggcgcc
     1381 gagcaggctg ggcgtgccgt cgcggtcgat gtagccctcg ccgagcgcga ggatgatgcc
     1441 cttgaagctc gaaccgccgc cctgcgtgat cttgccggtg acgatcagca acccggcgcc
     1501 gtggtcgccg cccttgattt cgaggtcgcc ttcgatgaag gtgaacttgg ggctggcctc
     1561 ggtgccgatg tcgccggggt tgagccgccc cagcgtggtg gcgcgctcct tcatgatatc
     1621 gacgaaaatg cgcgcgctgc cggctttctg gagccaccat tcgagtcccg acgggtcgag
     1681 cttgacgacc tgctgatcgc cgaggacgtt ggcctcgtcg ccgccgatgg ccttctcggc
     1741 ggagccttga tcggagtcct tgtcgaagcc gtcgcccgcg tcgaagtcgg cggtgttggt
     1801 gacggcgaag gccggcagcg cggggccggg cgggatggcg ctatcgttgc cgttccacag
     1861 gtgcgggtcg ctctcgccga catcgagcaa ctgcatcggc gtcgagtccg tgtcgtggct
     1921 gcggatggcg accgcggcgc gggccgtgaa ttcgaacggg tgggcgtcaa ccatcatccg
     1981 caagaccttg gtcgcgccct tcggcccgaa gccgcgcgag gtgatgatga ggaagcgcgg
     2041 gtcgcgctcg ggcggcaccg gcgccccttc ggcgagtccc gcgtcctgca cgctcagcgc
     2101 gtaggcgagc cgcggcgagt tcgagatcgg cacgacgccg ttcgtgcgcg gcagccagag
     2161 gtcgagcgtg ccggtgttgg cgcaggggtc ggacgtgccg cacacgaggt tgcggaaggt
     2221 ggccggggtg ccggcgggcg acgagggccg gttgccgcgg acagtcgaga gtgcggcctg
     2281 aagcccggcc tcggcggcgt agtaggcttg cgcctcggcg gtcgagtcgg cggcgttggc
     2341 ggcggacatg ccggtcgcca tcaggagcgc gccgcccgcg gccagcagca gcagcgaaat
     2401 cagcagcgac atgacgagcg ccgcgccgcg ctcgcgcggt cgctgacggt agagtcggca
     2461 gtctctctct ctcatctcgt gattcacctc tgtaactctt cggaagcggg cgccggtctt
     2521 tac
//
LOCUS       DBNGOH010000675         4358 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1017766, whole genome shotgun sequence.
ACCESSION   DBNGOH010000675 DBNGOH010000000
VERSION     DBNGOH010000675.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4358)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4358)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4358
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1017766"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1388)
                     /locus_tag="AC159P_13090"
     CDS             complement(<1..1388)
                     /locus_tag="AC159P_13090"
                     /inference="COORDINATES: protein motif:HMM:NF019350.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HIR3696429.1"
                     /translation="MTTRNDTPLSAEVPPPIESMDGRMVCLMRLVLALSALAIIYIDP
                     AEPDRMVGVTYAALVLYSVYSGLLCLLSYRRGSFPKFRAGHWIDTGWYLLLVALSSGT
                     NSIFFLFFFFSILVASFQWGFAEGLRVAFVSALLFTVTGLAAVPAGQPVELHRVLLRP
                     VYLLVLGYMIAYWGGFEIELKQRLALLKEVNTLSNPRFGVGHTVGSIMKRVRAFYDAD
                     ACLLVLRDSASGGYRLTRFARDAAAAEEARGERLPPELAAQFLALPESFAVVYDAGRR
                     LGPFGGGRYYACDLARGEQTGEGHETSAQIAVVLDADSFVSVPLFYRGQAAGRAYLAG
                     CRGDFRRPDVGFLLQIVEHVIPVLDNVRLLDRLASGAAEQERQKIARDIHDSVIQPYI
                     GLQYKVAAIRNKLAGSADVAPDLEHLFDVTVEEVTGLRRYVRGLKEEGTNRDDLLSAV
                     RRYVEQFKDHYG"
     gene            complement(1530..1853)
                     /locus_tag="AC159P_13095"
     CDS             complement(1530..1853)
                     /locus_tag="AC159P_13095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696430.1"
                     /translation="MDKEFKLRFGNDVAVGDGLKVKFSALVEDSRCPKGVDCIWEGNA
                     KIKVELVGAGKESASFELNTTVEPKSASGAGYEISLLKLDPYPNADARPKEKDYVATL
                     SVRRK"
     gene            complement(2050..3021)
                     /locus_tag="AC159P_13100"
     CDS             complement(2050..3021)
                     /locus_tag="AC159P_13100"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003872 - 6-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HIR3696431.1"
                     /translation="MKSIAVLTSGGDAPGMNAAIRAVVRKGLDLGWRVFGVRHGFSGL
                     IEGNLDPLGARDVGGIIQRGGTVLGSARCPEFKLEDGRREALRSLNERGVEGLVVIGG
                     NGSQTGSRELSRLGFPVVGVASTIDNDLYGSDITIGVDSALNVAVEAIDRLKITASSH
                     QRAVLVEVMGRDCGYIALMAAIAGGAEALVIPEVETDPETVAAELRTAYERGKAHAIV
                     VVAEGARYNAEGLSRYFKENQERLGFDLRTTILGHVQRGGAPGVFDRLLATRFGDAAV
                     RQLAAGTHGVLVGLLKGEISTTPLDEVVANKKPLDLRMLELERAMSS"
     gene            complement(3167..4303)
                     /locus_tag="AC159P_13105"
     CDS             complement(3167..4303)
                     /locus_tag="AC159P_13105"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011194646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HIR3696432.1"
                     /translation="MTEKIHIGVIYGGRSGEHEVSVRSACAVIESIDRARYDVTPLAI
                     TREGRWLSPAESAAMLPEAVRGLLPQAVNESAEALTIVGDPSHKGLLAIDGDERGRDL
                     RRRLDVVFPVLHGTYGEDGTIQGLLEMAGIPYVGCGVLASSCGMDKVTMKALFRQARL
                     PMCRHIWFLRAEWEREPSRVAARVAEEIGFPCFVKPANLGSSVGISRAAEASALAAAI
                     DLAARYDRKIIVEEALDVREIECAVMGNDEPEASLPGEYVVHDERAKFLDYTEKYSST
                     GHVDFVVPAPLDARLAKRIRRMAVKAFKAVDGAGFARVDFFLRRDTGKLLINELNTIP
                     GLTEVSGFPKMWDGSGVPFPHVIDRLVALAIERHADKTRNATSL"
BASE COUNT          720 a         1512 c         1445 g          681 t
ORIGIN      
        1 atgccgtagt gatccttgaa ctgttcgacg tagcggcgca cagccgagag caggtcgtcg
       61 cggttcgtgc cctcctcttt gaggccgcgc acgtagcggc gcaggcccgt cacctcctcg
      121 accgtcacgt cgaagaggtg ttcgaggtca ggggccacgt cggcgctgcc cgccagcttg
      181 ttgcggatgg ccgcgacctt gtactggaga ccgatgtagg gctgaatcac gctgtcgtgg
      241 atgtcgcgcg cgatcttctg ccgctcctgc tcggcagctc ccgaggcgag gcggtcgagc
      301 aggcgcacgt tgtcgagcac ggggatgacg tgctcgacga tctgcaagag gaagcccacg
      361 tcggggcggc ggaagtcgcc gcggcagccc gcgaggtacg cgcgccccgc cgcctgcccg
      421 cggtagaaca gcgggacgga gacgaacgag tccgcgtcga gcacgacggc gatctgcgcg
      481 ctcgtctcgt gcccctcgcc cgtctgctcg ccgcgcgcga ggtcgcacgc gtagtagcgc
      541 cccccgccga acggcccgag ccgccggccc gcgtcgtaga cgacggcgaa gctctcgggc
      601 agcgcgagga actgcgcggc cagctccggc ggcagccgct cgccgcgcgc ctcttccgcc
      661 gccgccgcgt cgcgcgcgaa gcgcgtcagc ctgtagccgc cgctcgcgga atcgcgcagg
      721 acgagcaggc acgcgtcggc gtcgtagaag gcgcgcacgc gcttcatgat cgagccgacg
      781 gtgtgcccca cgccgaagcg cgggttcgag agcgtgttga cctccttgag cagcgcgagc
      841 cgctgcttga gttcgatctc gaacccgccc cagtaggcga tcatgtagcc gagcacgagc
      901 aggtagacgg ggcgcaaaag gacgcggtgc agctcgacgg gctgcccggc gggcaccgcc
      961 gccagccccg tcaccgtgaa gagcagcgcc gagacgaagg ccacccgcag cccctccgca
     1021 aagccccact ggaacgaggc gacgaggatc gagaagaaga agaacaggaa gaagatgctg
     1081 ttcgtgccgc tgctgagcgc gacgagcagc agataccagc ccgtgtcgat ccagtggccc
     1141 gcgcggaatt tcgggaacga cccgcgccgg taagagagca ggcagaggag gccgctgtag
     1201 acgctgtaga gcacgagcgc cgcgtacgtg acgccgacca tgcggtcagg ctccgcgggg
     1261 tcgatataga tgatggcgag ggccgagagg gcgaggacga gcctcatcag gcagaccatc
     1321 cgaccgtcca tcgactcgat gggcggcggc acttccgcgg acagcggcgt gtcgttcctg
     1381 gtcgtcatct cgcgtcggcg acagacgtgg ccctacaccg gccaatcgat accgccggct
     1441 tcttcgcgcg gcatgagcgt agggttgtgg cgcgtgggga acgctcgggt gcagggagcg
     1501 cactcgccgg aggcgggaga aaggccgctt cacttccggc ggacagatag tgtggctacg
     1561 tagtccttct ccttggggcg cgcgtccgca ttcgggtagg ggtccagctt caacaggctg
     1621 atttcgtagc ccgcgccgga ggcgctcttc ggctcgacgg tcgtgttcag ctcgaacgaa
     1681 gcgctctctt tccccgcccc gaccagctcg actttgatct tcgcgttgcc ttcccagatg
     1741 cagtccacgc ccttcgggca gcgcgagtcc tcgacgaggg cgctgaactt gaccttcaac
     1801 ccgtcgccga cggcgacgtc gttgccgaag cgcagcttga actctttatc caaagaggcc
     1861 cctccgccgc atgcgggcgg cgacaccgcc cccgcgacga gtagtgacaa caaagccgag
     1921 gtgaataaac tcatgcgagc cgagcagcct ccacgcgccg taggttgatg cagccgccat
     1981 tataccagag gtttgccggc cagccccgcc gcgcaatcgc cgcgcgggcc tcggccccgc
     2041 ggtcgcgtgt cagctcgaca tcgcgcgttc cagttcgagc atccgcaggt cgagcggctt
     2101 cttgttggcg acgacctcgt cgagcggcgt ggtcgagatt tcgcccttga gcagtccgac
     2161 cagcacgccg tgcgtccccg cggccagttg cctcaccgcg gcgtcgccga accgcgtcgc
     2221 cagcaggcgg tcgaagacgc cgggcgcgcc gccccgctgc acgtgtccga ggatggtcgt
     2281 ccgcaaatcg aacccgagcc gctcctgatt ctccttgaag tagcgcgaca gcccctctgc
     2341 gttgtagcgc gcgccttcgg cgacgacgac gatggcgtgc gccttgccgc gctcgtaggc
     2401 cgtgcgcagc tccgcggcga ccgtctccgg gtcggtctcc acctcgggga tgaccaacgc
     2461 ctcggccccg cccgcgatgg ccgccatcag cgcgatgtaa ccgcagtcgc gccccatcac
     2521 ttcgacgagg acggcgcgct ggtgcgacga ggccgtgatc ttcaggcggt ctatcgcctc
     2581 gaccgcgacg ttgagcgccg agtccacgcc gatggtgatg tccgaaccgt agaggtcgtt
     2641 gtcgatggtc gaggcgaccc cgacgaccgg aaagccgagc cgggacagct cgcggctccc
     2701 ggtctgcgag ccgttcccgc cgatgacgac gagaccctcg acgccgcgct cgttgagcga
     2761 gcgcagggct tcgcggcgcc cgtcctccag cttgaactcc ggacagcgcg cgctgccgag
     2821 aacggtcccg ccacgctgga tgatcccgcc cacgtccctc gccccgagcg ggtcgaggtt
     2881 cccctcgatg agacccgaga agccgtggcg gacgccgaac acgcgccacc ccagatcgag
     2941 acccttccgc acgaccgccc tgatcgccgc gttcatgccg ggcgcgtcgc cgccgctcgt
     3001 cagcaccgcg atacttttca tgtcgccttc accttcaact tcaaagaagt cggaagtcag
     3061 aagtcagaag aaagaccttc ggtaacgaat cgtcagagta tcgtcgtcat ctcccgcgag
     3121 gcgaagccat gctgacttct gacttccgac ttctgacttc cgctcttcac aggctcgtcg
     3181 cgttgcgcgt cttgtcggcg tgtctttcga tggcgagtgc gacgagacgg tcgatgacgt
     3241 gcgggaacgg cacgcccgac ccgtcccaca tcttggggaa gccggagacc tccgtgaggc
     3301 cggggatggt gttcagctcg ttgatgagga gcttgcccgt gtcgcggcgc aggaagaagt
     3361 ccacgcgcgc gaagcccgcg ccgtccaccg ccttgaacgc cttgacggcc atgcgccgga
     3421 tgcgtttggc gagccgcgcg tccagcggcg cggggacgac gaaatcgacg tggcccgtgc
     3481 tcgaatactt ctccgtgtag tcgaggaact tcgcgcgctc gtcgtggacg acgtactcgc
     3541 cgggcaggct cgcctccggc tcgtcgttcc ccatcacggc gcattcgatc tccctcacgt
     3601 cgagcgcctc ttcgacgatg atcttccggt cgtagcgcgc cgcgaggtcg atggcggcgg
     3661 cgagcgcaga ggcttccgcc gcacgcgaga tgccgaccga cgagcctaag ttcgcgggct
     3721 tgacgaaaca ggggaagccg atctcctcgg cgacgcgcgc ggcgacccgt gaaggctcgc
     3781 gctcccactc cgcgcgcagg aaccagatgt ggcggcacat cggcagtcgc gcctgtcgga
     3841 agagcgcctt catcgtcacc ttgtccatgc cgcagctcga agccagcacg ccgcagccga
     3901 cgtaagggat gccggccatc tccagcaagc cctgtatcgt cccgtcctcg ccgtaggtgc
     3961 cgtgcagcac cgggaagacc acgtcgagac gcctgcgcaa gtcgcggcca cgttcgtcgc
     4021 cgtcgatggc gagcagcccc ttgtgcgaag ggtcgccgac gatggtcagc gcctcggcgg
     4081 actcgttgac cgcctgtggc agcaggccgc gcaccgcctc gggcagcatc gcggcggact
     4141 cggcgggcga cagccagcgc ccctcgcgcg tgatggcgag cggcgtcacg tcgtacctcg
     4201 cgcggtcgat gctctcgatc accgcgcagg ccgagcgcac ggacacttcg tgctcgcccg
     4261 agcgcccccc gtaaatcacc ccgatgtgaa ttttttccgt catggataaa aagtcgtgaa
     4321 ccgtgaaccg tgaaccgtga caagaaaaac cccgtgag
//
LOCUS       DBNGOH010000676         8373 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3581828, whole genome shotgun sequence.
ACCESSION   DBNGOH010000676 DBNGOH010000000
VERSION     DBNGOH010000676.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8373)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8373)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8373
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3581828"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1644)
                     /locus_tag="AC159P_13110"
     CDS             complement(<1..1644)
                     /locus_tag="AC159P_13110"
                     /inference="COORDINATES: protein motif:HMM:NF051698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR3696433.1"
                     /translation="MLLLLLSAASLCQAQTGSATLRVSVADPNKAVVPKAAVTITSER
                     TGEQRRAESNDDGDATFPSLDPGVYTVRVEVTNFKTAEQRGVTLSPNSTRGVDIQLEI
                     GVSTEVVTVVSNVPEIQTETGAKENTITAQQIDNLSIVSRSSLELLRILPGVVAPSPD
                     DPGFQSTSFNSGANANNNYNVNGLRGVNNSVSIDGSRVIDIGSNNGTIITANPDMVSE
                     VKVQTSNYAAEHGTSGVQITATTKGGGQDFHGTIYDYVRHHKLNANDRSRSSFNIERA
                     PEKYYYPGGNIGGPVCLPRFGEGGSVGKCYKDKLFFFFAFEVQRQTVDPGTSLARVPT
                     LEQRQGRGFFNRNSGNAPFNLDPALISPIGRALINLYPEPNFTESNCTGPNCRNYAFS
                     GLQPVNRTQANLRFDYKVNDKVSAYLRLARESEEQDYARGLWWSPSAYELPSHVRGTN
                     LGRSAALGITTVINPTMTNEIVLSASKLQLNNDYQDPDKVSLNSLGLSNLRGPFGQIS
                     QYAPIALITSWSGQTTGDLWEPGNLPLFAHNSSYSVYDNL"
     gene            2282..3019
                     /locus_tag="AC159P_13115"
     CDS             2282..3019
                     /locus_tag="AC159P_13115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696434.1"
                     /translation="MRRVLASAAFFGALLLLSLAASPAQPAVPSGPTQQMSSKDGKEG
                     GDDFVYADFENVQGNRAFSRRGGTVQLTSYHERTTSRFKGLADSSPPAPEVVRLKPDD
                     PNRAVAFDYELYSPNQYAGVGLEIQGQPYKDGKPVADDLSGYKNLLMQIYATGVTSLR
                     VEIMSRGQGINLSSGFPQSSFKIKPGFNTYKIPLDSLSQPSWVDTRVGAKDVLKKLTA
                     ISLTAYCERCTPVKGTVVVDNVVFQKQ"
     gene            complement(3146..4231)
                     /locus_tag="AC159P_13120"
     CDS             complement(3146..4231)
                     /locus_tag="AC159P_13120"
                     /EC_number="3.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012551.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031176 - endo-1,4-beta-xylanase activity
                     [Evidence IEA];
                     GO_process: GO:0045493 - xylan catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HIR3696435.1"
                     /translation="MNAMKALCVAAAFVSLITTGGGRCLSQPLRVHADRAGVLVGTAV
                     EPRLLSEPEYAATLAREFNMLTAENALKWGAIRPDRATLNFGPGDRLVAFAQKHNMKV
                     RGHTLLWSEYNPQWLVKGGFTPDEMSALLREHIEKVMKHYSGQVFAWDVVNEAFLADG
                     QIEPSVWYDQPGIGLKGKGTAYIEQALRWARAADPQALLFYNDYDTEGVNPKSDAVYA
                     MVKDFRARGVPIDGVGIQAHITDLEAKDLATLEANIARLAALGLQVHITEMDVGLPLD
                     AGGGLADKADLRRQADIYRRVADVCLRQPKCTAFQTWGFTDKYTWIPGFTKGVKGMPL
                     PFDRHYAAKPAYDALLDAFRQRARSAR"
     gene            complement(4302..5129)
                     /locus_tag="AC159P_13125"
     CDS             complement(4302..5129)
                     /locus_tag="AC159P_13125"
                     /inference="COORDINATES: protein motif:HMM:NF014116.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate binding domain-containing protein"
                     /protein_id="HIR3696436.1"
                     /translation="MFLLHRATLSAQLRRNVFARQACLLVAALASSLILLSPSTASAQ
                     TVVQNDFEDGTLQGWTPLGGGVILTNTTEAASTGARSLKTTGRTAGWTCSTPLVGDSG
                     PVQCTIDSMAAGAAAEFGLVVEVECPTPDGASIALSATAASATADPNPSPNNTSSASV
                     RVSNPPPVVMGLAVDKPVLEVPNHQLEDVALTYDISDNCDSALAPVITITSDQPVNGT
                     GDGDTAPDWEVVDAHHVRLRAERSPTSTAGRTYTITLTLAESAGSSTNSSVPVRVPR"
     gene            complement(5389..6120)
                     /locus_tag="AC159P_13130"
     CDS             complement(5389..6120)
                     /locus_tag="AC159P_13130"
                     /inference="COORDINATES: protein motif:HMM:NF013400.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH39 family glycosyl hydrolase"
                     /protein_id="HIR3696437.1"
                     /translation="MHAGQSAHFLEEKFATEPRQGDPVHDAYLSAAYIVSRLRGTEGH
                     AQSMSYWTFTDIIEENGPVPSPFHGGFGLLNFQGLRKPSFYAYQFLNRLGAEELVTDD
                     PGSWVTRGGGGAQVLFWDYTPPQTKESNQVYFKRDLPAQPSGNARVSLAGLPPGSYTL
                     NVYRVGYGVNDVYTDYFRMGSPPTLTREQVRLLAERNDGRPVSTALVEVKASGVFSRD
                     FPLRENDVYLITLLPRTPTKHRFRR"
     gene            complement(6159..7637)
                     /locus_tag="AC159P_13135"
     CDS             complement(6159..7637)
                     /locus_tag="AC159P_13135"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013572897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008422 - beta-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH1 family beta-glucosidase"
                     /protein_id="HIR3696438.1"
                     /translation="MRKNVSRRTFAKVAGAAALASATELPALASEVVPQGGAAVQAAA
                     LRAFPPGFLWGTATASYQVEGAVKEDGRGPSIWDTFSHTPGKVAGNANGDVATDHYHR
                     YRGDVQLMKALGVKAYRFSVAWPRVYPNGDGQPNPKGLDFYNRLVDELLANGIQPYAT
                     LYHWDLPQSLQDRFGGWEGRETSKAFADYAGYVAARLTDRVKHIFTINEFGAFVEVGY
                     RWGIHAPGLKLTGLRLNQTRHHAVLGHGLAVQAIRAAAKPGTKVGLAENITIAAPVIE
                     APAHIAAAERATREMNAQYLTVIQEGKYTDWYLKQAGADAPKFTPEDLQAISSPLDFV
                     GINVYTPQFVRADESSPVGFAHVPHPKSFPHAPSPWLFVAPEALYWGPRHVSKLWGAK
                     EIYITENGCSSADEPAPDGRVYDTDRVTYLRGYLTQLRRATAEGVPVRGYFLWSLMDN
                     FEWADGYTNRFGLHYVDYATQKRTPKLSADFYREVIARNAVV"
     gene            complement(7683..7781)
                     /locus_tag="AC159P_13140"
                     /pseudo
     CDS             complement(7683..7781)
                     /locus_tag="AC159P_13140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019385908.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            7834..8259
                     /locus_tag="AC159P_13145"
     CDS             7834..8259
                     /locus_tag="AC159P_13145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR3696439.1"
                     /translation="MFVAFMHSLPTHVREWGGPNGDQFLRMTANSNNLHPEYVSRRII
                     GLRYQQLVFDGPTGAIVSKVVFQHDLGGEFRQFRCALRKADEAALNSIYADDYAITTD
                     NGDVQTKVQRLAWVKANMARLSGLEFHVLKVRAAKRNRC"
BASE COUNT         1438 a         2640 c         2755 g         1540 t
ORIGIN      
        1 caggttgtcg tagaccgagt agctggagtt gtgcgcgaag agcgggaggt tgcccggctc
       61 ccagaggtcg cccgtcgtct ggccggacca gctcgtaatc agggcaatcg gcgcatactg
      121 actgatctgc ccgaacggcc cgcgcagatt ggagagaccg agcgaattca gactgacctt
      181 gtccggatcc tggtagtcgt tgttgagctg gagcttgctg gcgctcaaca cgatttcgtt
      241 ggtcatcgtc gggttgatga ccgtcgtgat gccgagtgcc gccgaccgac cgaggttggt
      301 gccgcggacg tgggacggca gctcgtaagc cgaaggactc caccacaggc cgcgcgcgta
      361 atcctgctcc tccgactcgc gcgccaggcg caggtacgcg ctcaccttgt cgttcacctt
      421 gtagtcgaag cgcaaattcg cctgcgtgcg gttcacgggc tggagtccgc tgaaggcgta
      481 gttccggcag ttggggccgg tgcagttcga ctcggtgaag ttcggctccg ggtacaggtt
      541 aatgagcgcc cgcccgatgg ggctgatcag cgccgggtcg aggttgaaag gcgcgttgcc
      601 gctgttccga ttgaagaagc cgcgcccctg ccgctgctcc agcgtgggga ctcgcgcgag
      661 actggttccg gggtctacgg tctgccgctg aacctcgaag gcgaaaaaga agaagagctt
      721 gtccttatag cacttcccga ccgacccgcc ctcgccgaac cgcggcaggc agaccggccc
      781 gccgatgttg ccgcccgggt aatagtattt ctccggcgcc ctctcgatgt tgaacgagct
      841 tctggaacgg tcgttcgcgt tgagcttgtg gtggcggacg tagtcgtaga tcgtgccgtg
      901 gaagtcttgc ccgccgccct tcgtggtggc cgtgatctgc acgccgctcg tcccgtgctc
      961 ggcggcgtag ttgctggtct ggactttgac ctccgacacc atgtcggggt tggccgtgat
     1021 gatcgtcccg ttgttggagc cgatgtcgat gacgcgcgag ccgtcgatgc tgacgctgtt
     1081 gttcacgccg cgcaggccgt tgacgttata gttgttgttg gcgttcgcgc ccgagttgaa
     1141 gctcgtgctc tggaaaccgg ggtcgtccgg gctgggggcg accacgccgg gcaggatgcg
     1201 caacagctcc aaggagctgc ggctcacgat cgacaggtta tcgatctgct gggccgtgat
     1261 cgtgttctcc ttcgcgcccg tctcggtctg gatttcgggc acgttcgaga ccaccgtgac
     1321 cacttcggtc gagacgccga tctccaactg gatgtccacg ccgcgcgtac tgttcgggct
     1381 taaggtgacg cccctctgct cggcggtctt gaaattcgtg acctcgaccc tgaccgtgta
     1441 gacgccgggg tcgagcgagg ggaaagtggc gtcgccgtcg tcgttgcttt cggccctgcg
     1501 ctgctcgccg gtccgctcgc tcgtgatcgt gacggcggcc ttcggcacca cggccttgtt
     1561 cgggtccgcg acggagacgc ggagcgtcgc gctgccggtc tgggcttggc agagagaggc
     1621 ggcagagagc agtagtaaga gcacgcacag cggtctcgga agtcgcgccg aagtttgccc
     1681 gccgcgccgc gccgcctgcc gacgacaccc cctattcgaa cggtctttaa aattcacggt
     1741 tagcatgcgg atgacctcct ggaagccgtt tgcaaccggc ctggcccaac tccggaaccc
     1801 ctgacgccga gcctaacaaa acctttcggc ggatttgagg cggccccaaa tcgccgacgg
     1861 ttcgacacag atgtcagagg ttgaattcgg gggacgcgtt ccctccgcag cgatgccacg
     1921 cgcgaaccct tacgactgag gagtgacggt cgaaaagggg aagaaagacg tgccggacgg
     1981 cgacatcacg gccttccccc ttagccccgg aaaacgcttg acgaacccgc gaaaagacct
     2041 gagatagata taaaagtggg ggcgacgtgc ctttgttgta cgacctaact aaagtcatta
     2101 aattacaagg cgatattctt gtcaagaact ttttatcgaa gccataatca aacttaagtt
     2161 ggagacggcg ggcggatgtt ttcacggctt gtcacggatg acttaagaga tatacattgc
     2221 gggccgcacc gttcgaccgg aggcccgccc cggcggtcaa tttctcgacg gagacgttca
     2281 catgagacgc gtactggctt ccgccgcttt cttcggcgcc ctcctgttgc tttcgctcgc
     2341 ggcgtccccg gcgcagcccg ccgtgccgtc cgggccgaca caacagatgt cttcaaagga
     2401 cggtaaggag ggaggcgacg acttcgtcta cgccgacttc gagaacgtgc agggtaaccg
     2461 cgcgttcagc cgccgcggcg gcaccgtgca gttgacctcg taccacgaaa gaactacgag
     2521 ccgcttcaag gggctggccg actcctcgcc gcccgccccc gaggtcgtcc gtctcaagcc
     2581 ggacgacccg aacagggccg tcgccttcga ttacgaactc tactcgccga accagtacgc
     2641 gggcgtcggc ctcgaaatac aaggacagcc ttacaaggac ggcaagcccg tggcggacga
     2701 cctgagcggc tacaaaaatt tgttgatgca gatctacgcc accggcgtga cctcgctgcg
     2761 ggtggagatc atgagtcggg ggcaaggcat caacctgtcg agcgggttcc cgcaatcatc
     2821 cttcaagatc aagcccggct tcaacaccta caagattccg ctcgactcgc tgtctcagcc
     2881 ctcctgggtg gacaccagag tcggcgccaa ggacgtgctc aaaaagctga cggccatcag
     2941 cctgaccgcg tactgcgagc gctgcacgcc ggtcaagggg acggtggtcg tagacaacgt
     3001 cgtcttccag aaacaataga ggcggcgggg tgtcggattt cagattggta acggtcagcc
     3061 gtcggggttc cgcattcggc aaaggcagga accaggctga gtgttgatcg ctgatcgctg
     3121 aatgctgcgt caatccgatt gaacctcagc gggcggagcg cgcccgctgg cggaaggcat
     3181 cgagcaaggc gtcgtaggcc ggcttcgcgg cgtagtgccg gtcgaacggc agcggcatcc
     3241 ccttcacccc tttcgtgaag cccggtatcc acgtgtactt gtctgtaaag ccccacgtct
     3301 ggaaggcggt gcatttgggt tgacgcaggc acacgtccgc cacgcgccgg tagatgtctg
     3361 cctgccgcct caagtccgcc ttgtccgcga gacccccgcc cgcatcgagc ggcagcccca
     3421 cgtccatctc cgtgatatgc acctgtaacc ccagcgccgc gagccgcgcg atgttcgctt
     3481 cgagcgtggc gaggtctttg gcttcgaggt ctgtgatgtg cgcctggatg ccgacgccgt
     3541 cgatgggcac gccgcgggca cggaagtcct tgaccatcgc gtagacggcg tccgacttcg
     3601 ggttcacgcc ttcggtgtcg tagtcgttgt agaagagcag cgcctgcggg tctgccgcgc
     3661 gcgcccagcg cagcgcctgt tcgatgtagg ccgtcccctt ccccttgagg ccgatgcccg
     3721 gctggtcgta ccagacggaa ggttctattt gaccgtccgc gaggaaagcc tcgttgacca
     3781 cgtcccacgc gaagacctgc ccggagtaat gcttcatcac cttctcgatg tgctcgcgca
     3841 gcagcgccga catctcgtcc ggcgtgaaac cgcccttcac gagccactgc gggttgtact
     3901 cgctccacag cagcgtgtgc ccgcgcacct tcatattatg cttctgcgcg aaagcgacga
     3961 ggcggtcgcc cggcccgaag ttgagggtgg cgcggtcggg gcggatggcg ccccacttca
     4021 acgcgttctc ggcggtcagc atgttgaact cgcgcgcgag cgtcgcggcg tactccggct
     4081 cggagagcag acgcggctcg acggcggtgc ccaccaatac gcccgcgcgg tctgcgtgca
     4141 cacgcagggg ttgtgagaga cacctgccgc cgcccgtcgt tatgagcgag acgaaggcgg
     4201 cggccacgca cagcgctttc attgcgttca tcggtcgcac cctttccgac gttatcggca
     4261 ggggcacacg ggctgctccg acccgtgtgc ccctgcggag gttaacgggg gacgcggact
     4321 ggcaccgagc tgttggtact cgacccggct gattctgcta acgtcagcgt gatggtgtac
     4381 gtgcggcccg cggtgctggt cggcgagcgc tcagcacgca ggcggacgtg gtgcgcgtca
     4441 accacctccc aatcgggggc cgtgtcgccg tcgccggtgc cgttgacggg ttggtcgctg
     4501 gtaatcgtga tgaccggggc gagggccgag tcacagttgt cgctgatgtc gtacgtgagc
     4561 gcgacatcct ccaactggtg gttggggact tcaagcacgg gcttgtccac cgcgaggccc
     4621 atgacgaccg gcggcgggtt ggagacccgg acgctcgccg acgacgtgtt gttcggcgat
     4681 gggttcgggt cggcggtggc cgaagcggcg gtggccgaaa gcgcgatcga cgcgccgtcc
     4741 ggcgtcgggc actcgacttc gaccacgagg ccgaactcag ccgcagcgcc cgcggccatc
     4801 gaatcaatcg tgcactggac ggggccggag tcgccgacca acggcgtcga acacgtccac
     4861 ccggcggtgc ggcccgtcgt cttcaggctc cgcgcgccgg tggatgccgc ctccgtcgtg
     4921 ttcgtcagga tgacgccccc gcctagcggc gtccaccctt gcagcgtccc gtcttcgaaa
     4981 tcgttctgca cgacggtctg cgcggacgcc gtcgaaggac tcagcaggat gagggaggac
     5041 gccagcgcgg cgacgagaag acacgcctgg cgggcgaaga cgttgcgccg aagctgagcc
     5101 gataaggtcg ctctgtgaag gagaaacatt ttcctgctcc ttgatattca ggaggttttg
     5161 gtatgacgga aactgtgtca gccggagccg ttagcagcgc cgcggactcc gggcgcgcga
     5221 caaatcctcc gcggctttga ttccgcacca gacctcgaca gaccgcgcgg cgcaggtgac
     5281 cttattctcc ccgtctctgg cgagaccgga atataccacc acttttgtcg ggccatgcaa
     5341 caaaagttgg acgcacgccc gcgaaaaggt gacgcgcctg cgcggcactc accgcctgaa
     5401 cctgtgttta gtgggcgtcc ggggtagtag cgtgatgagg tacacgtcgt tctcccgcag
     5461 ggggaagtcg cgggagaaaa cgccggaggc tttgacctcg acgagcgccg tggagacggg
     5521 ccgcccgtcg ttgcgttccg cgaggaggcg cacctgctcg cgcgtgagcg tcggcggcga
     5581 gcccatcctg aagtagtcgg tgtaaacgtc gttcacgccg tagccgacgc ggtagacgtt
     5641 caacgtgtag ctgccgggcg gcagccccgc gagcgagacg cgcgcgttgc ccgagggctg
     5701 cgccgggagg tcgcgcttga agtagacctg gttgctctcc ttcgtctgcg gcggcgtgta
     5761 gtcccagaag agcacctgcg cgccgccccc gccgcgcgtc acccagctac ccggatcgtc
     5821 cgtcacaagc tcctccgcgc cgagccggtt caggaactgg taggcgtaga aggagggctt
     5881 gcgtaagccc tgaaagttga gcagcccgaa gccgccgtgg aagggcgacg gcacggggcc
     5941 gttctcctcg atgatgtcgg tgaacgtcca gtaggacatc gactgggcgt ggccctccgt
     6001 tcccctgagg cgcgagacga tgtaggcggc cgacaggtag gcgtcgtgga cggggtcgcc
     6061 ctgtcggggt tccgttgcaa acttctcttc caggaagtgc gcactctgcc cggcgtgcac
     6121 gtccgggcta aaccgttcgt ctgcgtctga gacccgcctc acacgacggc gttacgcgcg
     6181 atgacctcgc ggtagaagtc cgcgctcagc ttgggcgttc gcttctgcgt cgcgtagtcc
     6241 acgtagtgga gaccgaagcg gttggtgtag ccgtcggccc actcgaagtt gtccatcagg
     6301 ctccacagga agtagccgcg cacgggcacg ccctcggcgg tcgcgcggcg cagttgcgtg
     6361 aggtagccgc gcaggtacgt cacgcggtcg gtgtcgtaga cgcgcccgtc cggcgccggc
     6421 tcgtccgcgg acgagcagcc gttctccgtg atgtaaatct ccttcgcgcc ccacagcttc
     6481 gagacgtggc gcggccccca gtagagcgcc tccggcgcga cgaagagcca cggcgacggc
     6541 gcgtgcggga aagacttcgg gtgcggcacg tgggcgaagc cgacgggcga agactcgtcg
     6601 gcgcgcacga actgcggcgt gtagacgttg atgccgacga agtcgagcgg cgacgagatc
     6661 gcctgcaaat cttccggcgt gaacttcggc gcgtcggccc cggcctgctt gagataccag
     6721 tcggtgtact tcccttcctg aatcacggtc aggtactgcg cgttcatctc gcgcgtggcg
     6781 cgctcggctg ccgcgatgtg agcgggcgcc tcgatgaccg gggcggcgat ggtgatgttc
     6841 tcggccaagc ccactttcgt ccccggcttt gccgcggcgc ggatggcctg caccgccagc
     6901 ccgtggccga gcacggcgtg gtggcgcgtc tggttcaggc gcaggcccgt cagcttcagg
     6961 cccggcgcgt ggatgcccca gcgatagccg acctcgacga aggcgccgaa ctcgttgatg
     7021 gtgaagatgt gcttgacgcg gtcggtcagg cgggccgcga cgtagcccgc gtagtcggcg
     7081 aacgccttcg acgtctcgcg cccctcccag ccgccgaagc ggtcttgcaa ggactgcggc
     7141 aggtcccagt ggtagagcgt ggcgtagggc tggatgccgt tggcgagcag ctcgtccaca
     7201 agccggttgt aaaagtcgag acccttcggg ttcggctggc cgtcgccgtt ggggtagacg
     7261 cgtggccacg cgacggagaa gcggtacgcc ttaacgccga gcgccttcat caactgcacg
     7321 tcgccccggt agcggtgata gtggtctgtc gccacgtcgc cgttggcgtt acccgcgacc
     7381 ttgccgggcg tgtgcgagaa ggtgtcccag atggaaggcc cccgtccgtc ctccttgacg
     7441 gcgccctcca cctgatagga ggcggtcgcc gtgccccaga ggaagccggg cgggaacgcg
     7501 cgcaacgcgg cggcctgcac cgccgcgccg ccctggggca cgacctcgga ggcgagcgcc
     7561 ggcagttcgg tcgcggaggc gagcgccgcg gcgcccgcca cctttgcgaa tgtgcgtctc
     7621 gaaacattct tgcgcaacag tcaatccctc ccgtcttgtg aatatctaag gggcgagagt
     7681 aattaaagcg gatattttct tagcagcttc aacaactcag cgtacacgtt gttctttctc
     7741 aagttgaacc tatattcgca caatttcaag tgtaggtaga acggctttgc ctatttattc
     7801 ggcaaacggc gcatacatcg gttgatatgc tgcatgttcg tggcctttat gcacagcctg
     7861 ccgacccacg tccgcgagtg gggcgggccg aacggcgacc agttcctgcg gatgaccgct
     7921 aacagcaaca acctccaccc cgagtacgtc agccgccgca tcatcgggct gcgctaccag
     7981 cagctcgtct tcgacgggcc gaccggcgcc atcgtctcga aggtcgtctt ccagcacgac
     8041 ctcggtggcg agttccgcca gttccggtgc gccctgcgca aggccgacga ggcggccctc
     8101 aactcgatct acgccgacga ctacgccatc acgaccgaca acggcgacgt gcagacaaag
     8161 gtgcaacggc ttgcgtgggt caaggcgaac atggcgcggc tgagcgggct tgagtttcat
     8221 gtcttgaagg tgcgagccgc aaaacgcaac cgctgttaac gttcgcacgc ccgacgagac
     8281 agacgcacac ggcgtcaagt tttcgagaac cggattcata accctgaaaa ggtaatggcg
     8341 agagtgtcgc cattaccttt tcaccattga tca
//
LOCUS       DBNGOH010000677         3073 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3950572, whole genome shotgun sequence.
ACCESSION   DBNGOH010000677 DBNGOH010000000
VERSION     DBNGOH010000677.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3073)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3073)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3073
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3950572"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            43..1407
                     /locus_tag="AC159P_13150"
     CDS             43..1407
                     /locus_tag="AC159P_13150"
                     /inference="COORDINATES: protein motif:HMM:NF039204.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5715 family protein"
                     /protein_id="HIR3696440.1"
                     /translation="MEKRETWKAWGPLCLMLAALVACASLLPGVKAPGREGVSAFLPT
                     LPALGGPAGAAGRSTWAVAAAKVKEERGEPTGRQAEVDVPEQLKHYSDRRRFLGIQTA
                     EWKEHDITTPQDFAGLARLISAGELVEVAQVSENHVLYGVGALADEGRFTHYDEAKGK
                     RVALYADEELAGEYARLEGERAGVKRELDALRAELKSLGKRERTQRKKLLSETAAKEK
                     SLKTLRERKELLDTFYGAAAGRRRLASEYEAVASLARDFAGRSYDAGEAASRKQMKVR
                     MLSHLRPEALRVLEELARSYKERFDRPLPVTSLVRPDEYQRQLGAVNANATRIETPPH
                     STGLAFDILYRYMTAEEQAHVMADIARLRDAGRVEALRENRDHFHVFAFLDGQRPGED
                     LIRASLGDAKPADARPRGEKRADAPTTKKKDTKTAKAAGRKADKKQTAKKETAKRRGR
                     GGRA"
     gene            1646..2881
                     /gene="serA"
                     /locus_tag="AC159P_13155"
     CDS             1646..2881
                     /gene="serA"
                     /locus_tag="AC159P_13155"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HIR3696441.1"
                     /translation="MKSKETSYPRDKIKILLLENISASAIGAFEGGGYVGIRKIPGAL
                     GEKELIAALKGVHILGIRSKTRVTERVLAEADRLLAVGCFCIGVNQVDLKAATRAGVA
                     VFNAPYSNTRSVAELVIGLCVMLIRRVADKNAAAHRGVWLKESSGSYELRGKTLGVVG
                     YGNIGSQVSVMAEALGMKVVYYDVVPKLPHGNAVQARDLAEVLKTADVVTLHVPGGAS
                     TRNMIDERTLAHLRPGAILINYSRGDVVDLDALKKYIDGGVVAGAAIDVFPEEPVRSG
                     DPFSTVLQNLPNVILTPHIGGSTEEAQAGIGLDVSTKLVQYLELGASTGSHTVPPVSL
                     PPQEGTHRILHIHRNVPGVLGEINSKLSARGINILGQYLKTNDEIGYVILDVDTKLSR
                     AAHEILREVKGTVKTRVVY"
     gene            2922..3073
                     /locus_tag="AC159P_13160"
                     /pseudo
     CDS             2922..3073
                     /locus_tag="AC159P_13160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013291146.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor family protein"
BASE COUNT          573 a          982 c         1069 g          449 t
ORIGIN      
        1 ctgttcgtca ggcccttttt cttttcaggg ggaacctcgc acgtggaaaa aagggagacg
       61 tggaaggcgt gggggccttt gtgcctgatg ttggccgctc tggtcgcgtg cgcgtcgctg
      121 ttgccgggcg tgaaggcgcc gggccgcgaa ggcgtctcgg cgttcctgcc gacgctgccc
      181 gcgctgggcg ggcctgccgg ggccgccggg cgcagcacgt gggccgtggc ggcggcgaag
      241 gtgaaggagg agcggggcga gccgacgggc cggcaggccg aggtcgatgt ccccgaacaa
      301 ctcaagcact acagcgaccg ccgacgcttc ctcggcatcc agaccgccga gtggaaggag
      361 cacgacatca cgacgccgca ggacttcgcg ggcctcgcgc gcctcatcag cgcgggcgag
      421 ctggtcgagg tcgcgcaggt ctcagagaac catgtgctct acggcgtcgg cgccctcgcc
      481 gacgaaggcc gcttcacgca ctacgacgag gcgaagggca agcgcgtcgc gctctacgct
      541 gacgaagagc tggcgggcga gtacgcgcgg ctcgaaggag agagggcggg cgtcaagcgc
      601 gagctggacg cgctccgcgc ggagctgaag tcgctcggca agcgcgagcg cacgcagcgc
      661 aagaagctgc tgtccgagac cgccgcgaag gagaagtctc tgaagaccct gcgcgagcgt
      721 aaagagctgc tcgacacgtt ctacggcgcc gcggcggggc gccgccgcct cgcgagcgag
      781 tacgaagccg tcgcgtcgct cgcgcgcgac ttcgcgggcc gcagctacga cgcgggcgag
      841 gccgcctcgc gcaagcagat gaaggtgcgg atgctcagcc acctgcggcc cgaggcgctg
      901 cgggtgctgg aagagctggc gcgctcgtac aaagagaggt tcgaccggcc cttgcccgtc
      961 acctcgctcg tccggcccga cgagtaccag cggcagctcg gcgcggtcaa cgcgaacgcg
     1021 acgcgcatcg agacgccgcc gcactcgacc ggcctcgcct tcgacatcct ctaccgctac
     1081 atgaccgcag aggagcaggc gcacgtcatg gccgacatcg cgcggctgcg cgacgcgggc
     1141 cgcgtcgaag cgctccgcga gaaccgcgac cacttccacg tcttcgcctt cctcgacggc
     1201 cagcgccccg gcgaagacct catccgcgcc tcgctcggcg acgccaagcc cgccgacgcc
     1261 aggccgcgcg gagagaagcg cgccgacgcg ccgacgacga agaagaaaga caccaagacc
     1321 gcgaaggccg ccggcaggaa ggccgataaa aaacagactg ctaaaaagga gaccgccaag
     1381 cgacgcggca ggggcgggcg cgcgtaggaa gtgagcagtg agcagtaagc tgtgagcagt
     1441 taaaaaacgg aagctggaag ataaaagaga gaagaggctt gggaggtaaa gtcccaagcc
     1501 tcttctctct ttcgtcttca gacttcagac tttagacctt agacttcgga cttcttctac
     1561 tgctcaccgc tcaccgctta ctgctcactt cttgtgctac tctcttcacg cacttcatct
     1621 tatatgtccg gttcggtctt cgttcatgaa gagtaaagag acatcctacc cgcgggataa
     1681 gatcaaaatc ctgctgctcg aaaacatcag cgcctcggcc atcggggcgt tcgagggtgg
     1741 cgggtacgtc gggatcagga aaatccccgg cgcgctcggc gagaaggagt tgatcgcggc
     1801 gctgaagggc gtgcacatcc tcggcatccg ctcgaagacg cgcgtcacgg agagggtcct
     1861 ggcggaggcc gaccggctgc tcgccgtcgg ctgcttctgc atcggcgtga atcaggtcga
     1921 tctgaaggcg gcgacccgcg cgggcgtcgc cgtcttcaac gcgccgtact cgaacacgcg
     1981 ttcggtggcg gagctggtga tcgggctgtg cgtgatgctg attcgcaggg tggcggacaa
     2041 gaacgcggcg gcgcacagag gggtgtggct gaaggagtcg agcgggagct acgagctgcg
     2101 cggcaagacg ctcggcgtgg tcggctacgg caacatcggc tcgcaggtct cggtcatggc
     2161 cgaggcgctg gggatgaagg tcgtctacta cgacgtggtg ccgaagctcc cgcacggcaa
     2221 cgccgtgcag gcgcgcgacc tcgcggaggt gttgaagacg gcggacgtgg tgacgctcca
     2281 cgtgccgggc ggcgcttcga cgcgcaacat gatcgacgag cgcacgctcg cgcacctgcg
     2341 gcccggcgcc atcctcatca actacagccg gggcgacgtg gtcgatctcg atgcgctcaa
     2401 aaaatacatc gacggcgggg tggtggcggg cgcggcaatc gacgtctttc cggaagagcc
     2461 ggtgcgcagc ggcgacccgt tctcgaccgt cctgcaaaac ctgccgaacg tgatcctgac
     2521 gccgcacatc ggcggctcga ccgaagaggc gcaggccggc atcgggctgg acgtttccac
     2581 gaagctcgtc cagtacctcg aactcggcgc ctcgaccggg tcgcacaccg tcccgcccgt
     2641 cagcctgccg ccgcaggagg gcacgcaccg catcctgcac atccaccgca acgtgccggg
     2701 cgtgctcggc gagatcaact cgaagctctc cgcgcgcggc atcaacatcc tcggccagta
     2761 cctcaagacg aacgacgaga tcggctacgt catcctcgac gtggacacga agctctctcg
     2821 cgccgcgcac gaaattctaa gggaagtgaa ggggacggtg aagacgaggg tggtgtactg
     2881 atgggcggtg tgtgtgcgac ggcggcgcgc gttgcgaggt ggctatgatc gtcttgcccg
     2941 aatcggacga agaccttttg cacgagtgcg aggtcgagac gttccgcgcg agcggccccg
     3001 gcgggcagca cgtgaacaag acggaaagcg ccgtgcggct caggcacctg ccgacggggc
     3061 tggtcgccac ctc
//
LOCUS       DBNGOH010000678         2957 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1018224, whole genome shotgun sequence.
ACCESSION   DBNGOH010000678 DBNGOH010000000
VERSION     DBNGOH010000678.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2957)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2957)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2957
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1018224"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2773
                     /locus_tag="AC159P_13165"
     CDS             <1..2773
                     /locus_tag="AC159P_13165"
                     /inference="COORDINATES: protein motif:HMM:NF018905.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="galactose oxidase-like domain-containing
                     protein"
                     /protein_id="HIR3696442.1"
                     /translation="ADINEPPFEHRVPGAVIYTRPGTWLRIVVRNDDDAPHSLHLHGL
                     RHGIDSDGAWPFGTQAAGAARSDEICPGQTWTYTFEVTEYLIGAWPFHDHYRDIEKSV
                     NRGLFGAVIVLPEKDFRALPRFRLPDVLLKMVRGELAAARPVVRAAPARVSHSHQPAA
                     HIRRDPTAPTRGGHKEDESHAESEARDEALEQQLVLLAELAHAPQPMPKPTEPLYVPL
                     FIHVMQGEQEEEEEEENGGGRGDVVDVRPIDLQSVDFVLRRDNVFVLRRADDLVGGLD
                     DLVVRPDVLDAGRARLSRRSLCFNGRTFVGNTPTILATAGQRIRWYVFNLDLGQQGWH
                     NFHPHGQRWRFANETIAVRSLGPAEAFVVETNAPPVLLLPPRIERYQAPRYRPRDARL
                     FKLRGDFPFHCHSADDMQQGLVGLVRSTQEVWLTPQDAALIRSRTGLPLDAGDNDCPA
                     VDFERCANAANGKWEEVPGVAGVTFMHAVLLANTRRVLFWGYNRPDQTRVWDQATGAY
                     DQPFNQPISISPDTDLWSGSHAYLNDAAGTILIHGGYSLGLTPPFTVDTERRTFLFDP
                     PSLTWVDAADTTLNRFYPSTVTLADGRVMTLFGQNTVSGVTSSAMEIFTPGGAGAWSA
                     PEPLPFNFFYYPWTFVLPDGEIFIAGPQKPARRFDPTVLPVVDDPARQFDQVFSQRGV
                     NMNGTAALLPLRPPNYEPRVLIAGGNGPDTGQSAELIDLSVPAPAWQALPNMNIARDN
                     MNCVLLPDGKLIVLGGTLGGPDGGPVETFDPDNPAAGFVAGPSMAHERGYHSAALLLA
                     DGSVLVGGDPGGDFTPHERYLPPYFFAARPTITGAPANIAYGAGFTVNTPEAAEIAEV
                     VLMRPGAVTHAFNMSQRHVGCAIVSAAGNSVQATAPPDGNVAPPGYYLLFVVNGDRVP
                     SEGVWIRLS"
BASE COUNT          480 a         1075 c          930 g          472 t
ORIGIN      
        1 ggccgacatc aacgaacctc ccttcgaaca ccgcgtgccg ggcgcggtca tctacacgcg
       61 ccccggcacc tggctccgca tcgtcgtcag gaacgacgac gatgcgccgc acagcctcca
      121 cctccacggg ctgcgccacg gcatagactc ggacggcgcg tggcccttcg gcacgcaggc
      181 cgcgggcgcc gcccgctccg acgaaatctg tcccggccag acgtggacgt acaccttcga
      241 agtcaccgag tacctgatcg gcgcatggcc gttccacgac cactaccgcg acatcgagaa
      301 gagcgtcaac cgcggcctct tcggcgccgt catcgtcctg cccgaaaaag atttcagagc
      361 tttgccgcgc ttccgtctgc cggacgtgct gctgaagatg gtgcgcggcg agctggcggc
      421 ggcgcgtccg gtcgtccgcg cggctcccgc gcgcgtgagc cacagtcatc agcccgctgc
      481 gcacatacgc cgcgacccca cggcccccac gcgcgggggc cacaaggagg acgagtcgca
      541 cgcggagagc gaggcgcgcg atgaggctct cgaacagcaa ctggtgctgc tcgctgaact
      601 cgcccacgcg ccgcagccga tgcccaaacc gaccgagccg ctctacgtgc ccctcttcat
      661 ccacgtcatg cagggagaac aggaggagga ggaagaggaa gagaacggcg gcgggcgggg
      721 cgacgttgtt gacgtgaggc ccattgactt gcagtccgtg gactttgtct tgagacgcga
      781 taacgtcttc gtcttgaggc gcgccgacga tctcgtcggg gggcttgacg atctcgtcgt
      841 gcggcccgac gtgctcgacg cggggcgcgc gcgactcagc cggcgctcgc tgtgcttcaa
      901 cgggcgcacg ttcgtcggca acacgccgac cattctggcc accgcggggc agcggattcg
      961 ctggtacgtc ttcaacctcg atctcggtca gcagggctgg cacaacttcc acccgcacgg
     1021 gcagcgttgg cggttcgcca acgagaccat cgccgtgcgc agcctcggcc ccgcggaggc
     1081 tttcgtcgtc gagaccaacg cgccgcccgt cttgctgctc ccgccccgca tcgaaaggta
     1141 tcaggccccg cgctaccgtc cgcgcgacgc caggctcttc aagctgcgcg gcgacttccc
     1201 cttccactgt cactccgcgg acgacatgca gcagggactc gtcgggctgg tgcgctcgac
     1261 gcaagaagtg tggctgacgc cgcaggacgc ggccctcatc aggtcgcgca cggggctgcc
     1321 gctcgacgcg ggcgacaacg actgccccgc cgtggacttc gaacgctgcg cgaacgccgc
     1381 gaacggcaag tgggaagagg tgccgggcgt ggcgggcgtc accttcatgc acgcggtgct
     1441 gctcgcgaac acgaggcgcg tgctgttctg gggatacaac cggcccgacc agacgcgcgt
     1501 ctgggatcag gcgaccggcg cttacgatca gccctttaac cagcccatat ccatctcgcc
     1561 ggatactgac ctctggtcgg gcagccacgc ttatttgaac gacgcggcgg gcaccatcct
     1621 catccacggc ggttactcgt tgggcctgac cccgccgttc accgttgaca cggagcggcg
     1681 cacgttcctg ttcgaccctc cgtccctgac ttgggttgac gccgccgaca ccaccctgaa
     1741 ccggttctac ccatcaaccg tcacgctggc ggacgggcgc gtgatgacgc tgttcggcca
     1801 gaacaccgtc agcggcgtca cgtcgagcgc gatggagatc ttcacgcccg gcggcgcggg
     1861 cgcttggtcc gcacccgaac cgctgccctt caactttttc tactacccgt ggacgttcgt
     1921 gctgcccgat ggcgagattt tcatcgccgg gccgcagaag cccgcgcggc gtttcgaccc
     1981 gacggtgctg cccgtcgtgg acgaccccgc ccgacagttc gaccaggtct tctcgcagcg
     2041 cggcgtcaac atgaacggga cggcggcgct gctccccttg cggcctccca actacgagcc
     2101 gcgcgtgctc atcgcgggcg gcaacggccc cgacaccggg cagtctgcgg agttgatcga
     2161 cctctccgtg ccggcgcccg cgtggcaggc gctcccgaac atgaacatcg cgcgcgacaa
     2221 catgaactgc gtgctgctgc ccgacggcaa gctgatcgtc ctcggcggga cgctcggcgg
     2281 gccggacggc ggcccggtcg agacgttcga ccccgacaac ccggctgcgg gcttcgtggc
     2341 cggcccttcg atggcgcacg agcgcggcta ccactcggcg gcgctgctgc tcgccgacgg
     2401 cagcgtactc gtcggcggcg accccggcgg cgacttcacg ccgcacgaac gctacctgcc
     2461 gccctacttc ttcgcggccc ggcccaccat caccggcgcc ccggccaaca tcgcctacgg
     2521 cgccggcttc acggtcaaca cgccggaggc cgcggagatc gccgaggtgg tgctgatgcg
     2581 gcccggcgcc gtgacgcacg ccttcaacat gtcgcagcgc catgtcggct gcgccatcgt
     2641 ctccgccgcg ggcaactccg tccaggccac cgcgcccccg gacggcaacg tggcgcctcc
     2701 ggggtattac ctgctcttcg tcgtcaacgg cgaccgggtg ccctcggaag gcgtctggat
     2761 caggctgagt tgaacgttga gtttaccgtg cgttcatttc gacacagagg tcacggagga
     2821 gacacggagt ccacagagca aaccattcgt cttctccgtg gactctgtgg aacctctgtg
     2881 ttctctgtgt tgaagtgaat ctgcccgaca cccaaccgca aacttctctt aacccacaat
     2941 ccacaatcca caatcca
//
LOCUS       DBNGOH010000679         2250 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3033149, whole genome shotgun sequence.
ACCESSION   DBNGOH010000679 DBNGOH010000000
VERSION     DBNGOH010000679.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2250)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2250)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2250
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3033149"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(83..532)
                     /locus_tag="AC159P_13170"
     CDS             complement(83..532)
                     /locus_tag="AC159P_13170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696443.1"
                     /translation="MKRLFAPPAITVLLALFLLAGAPPPAGAQDGGGDAIIIENKHIL
                     MMVAAKIAPDVILAKIRTSPCNFDTFPPVLAELKSKGVPDSILLAMVRAPHGPSSAEQ
                     DAKGDVNLHPVSEVIKYSGNYVKTRQTPRPARTRPSTRSGIGVRRRS"
     gene            872..>2250
                     /locus_tag="AC159P_13175"
     CDS             872..>2250
                     /locus_tag="AC159P_13175"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3696444.1"
                     /translation="MSQNDDITILVVNDSPDHLSLMGMALSKAGFGVLTASDGAEAFG
                     IAEREHIEIIVSDVMMPNVSGIELCRLVRGHPGLKWVPILLVSALRKDTATVVEGLRS
                     GADGYLESPYDPAHLIAAVARLVERKRAEDMIRDMNEELEVRVAERTAQLAKANRELE
                     REVAERKLVESALEKARDAALEAARLKSEFLANVSHEIRTPMHAISGMVGLLLGTPLK
                     PRQREYAEVIKEGSESLLTVINDVLDLSKIEAGKLRVEKSDFDLTDVVNGAAQLFAER
                     AGAKGLELSAAVGADVPVLLRGDPGRLRQVLTNLLSNAVKFTARGAVAVRVNRVTEQD
                     RRVTLRFEVSDTGIGVRPEAQRRIFLPFAQADGSVTRAYGGTGLGLAISRQLVELMGG
                     EIGVESEGGRGSTFWFTVRLGKQPVAEAPPHGASGLDGIRVLVVDDNPNNRETLLRHA
                     RMWDMRPAAA"
BASE COUNT          373 a          736 c          772 g          369 t
ORIGIN      
        1 aaagttttct ctgcgccctc tgcgcctctt cgcgcactcc gcgttgaaat gaacgcccgg
       61 tacgcttcaa ccgcaatccg ctctagctcc ttctccttac tccgatgccg ctccgcgtgg
      121 agggccgcgt tctggccggc ctcggggtct gccgcgtctt gacgtagttg cccgaatact
      181 tgatgacctc ggagacgggg tggaggttga catcgccctt cgcgtcctgc tcggcgctcg
      241 aagggccgtg cggcgccctg accatggcca gcaggatgga atcgggcacg cccttggatt
      301 tcaactcggc cagcacgggc gggaacgtat cgaagttgca gggcgaggtg cggattttcg
      361 cgagaatgac atcgggggcg atcttggccg cgaccatcat gagaatgtgc ttgttctcga
      421 tgatgatggc gtctccgccg ccgtcctgcg cgcccgccgg cggcggggca ccggcaagca
      481 ggaacagggc gagcaggacg gtgatggcgg gcggggcaaa gaggcgtttc atgggggtct
      541 ccttctgacc gtgatcgcgg cctataacgg acgcgacgaa aaactgttcg gtcgcacaga
      601 ataacccgga cgggcggcgc cacacatcgg actttcgtcc caattcgcgc gaggaatcgg
      661 cggcacattt gtgcctttca tcctggaagg ttttcgatta aggtatcgaa aaatcgcccg
      721 ggccggggcc gcccggcctt tctcttagga cagccgcgga gcgctataat agtgacgtcg
      781 gtattttccc ttccgccggg gccggggccg ttcaagcccc tttcgaccgg tcgccccgac
      841 gcccttgatt tctccctgag gggttcgcgc attgtcacag aatgacgaca tcaccatcct
      901 cgtcgtgaac gactcgcccg accacctgtc gctgatgggc atggcgctca gtaaggcagg
      961 cttcggggtg ctgaccgcct ccgacggggc cgaggcgttc ggcatcgccg agcgcgagca
     1021 catcgagatc atcgtcagcg acgtgatgat gccgaacgtc agcggcatcg agctgtgccg
     1081 cctcgtgcgg ggccaccccg gtctgaagtg ggttccgatc ctgctcgtga gcgcgctcag
     1141 gaaggacacg gcgaccgtcg tcgagggcct ccgctcgggc gccgacggct acctcgaaag
     1201 cccttacgac ccggcgcacc tgatcgcggc ggtcgcccgc ctcgtcgagc gcaagcgcgc
     1261 cgaagacatg atccgcgaca tgaacgagga gctggaggtg agggtcgcgg agcggacggc
     1321 gcagctcgcc aaggcgaaca gggagctgga acgcgaggtc gccgagcgga agctcgtcga
     1381 atcggcgctg gagaaggcgc gcgacgcggc gctggaagcc gcgcggctga agtccgagtt
     1441 tttggccaac gtcagccacg agatacgcac gcccatgcac gccatctcgg gcatggtcgg
     1501 gctgctgctc ggcacgccgc tcaagccccg gcagcgcgag tacgccgaag tcatcaagga
     1561 gggttcggag tcgctgctga cggtcatcaa cgacgtgctc gacctctcga agatcgaggc
     1621 cgggaaactg cgcgtcgaga agagcgactt cgacctgacg gacgtggtca acggtgcggc
     1681 gcagctcttc gccgagaggg ccggggcgaa agggctggag ctgagcgcgg cggtcggcgc
     1741 cgatgtcccc gtcctcttgc ggggcgatcc gggccggctg cgacaggtgt tgacgaacct
     1801 cctgagcaac gccgtcaagt tcaccgcccg cggcgcggtc gccgtccgcg tcaaccgggt
     1861 caccgagcaa gaccgccgcg tgaccctccg gttcgaggtg agcgacaccg gcatcggggt
     1921 caggcccgag gcgcaacgcc gcatcttcct ccccttcgcg caggccgacg gctccgtcac
     1981 gcgcgcctac gggggcaccg gtctcggact cgccatctcg cggcagctcg tcgagctgat
     2041 ggggggcgag atcggcgtgg agagcgaggg tgggcgcggc tcgaccttct ggttcaccgt
     2101 caggctcggc aaacagccgg tcgcggaggc gccgccgcac ggcgcctccg gcctcgacgg
     2161 catccgggtg ctcgtcgtgg acgacaaccc gaacaaccgc gagacgctcc tgcgccacgc
     2221 gcggatgtgg gacatgcgcc ccgccgccgc
//
LOCUS       DBNGOH010000680         3189 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4866311, whole genome shotgun sequence.
ACCESSION   DBNGOH010000680 DBNGOH010000000
VERSION     DBNGOH010000680.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3189)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3189)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3189
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4866311"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(125..376)
                     /locus_tag="AC159P_13180"
     CDS             complement(125..376)
                     /locus_tag="AC159P_13180"
                     /EC_number="1.8.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012675.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HIR3696445.1"
                     /translation="MLTVKVYGADWCGDTRHTREFLDGLGVAYEYVDVEQDADAAAWV
                     REHNDGKERKPTLDVAGQVLSVPSDHELTQALREKGMMA"
     gene            567..2009
                     /gene="purF"
                     /locus_tag="AC159P_13185"
     CDS             567..2009
                     /gene="purF"
                     /locus_tag="AC159P_13185"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HIR3696446.1"
                     /translation="MTDGAGEMERDKFRDECGVFGIFGNAEAARMTYLGLYQLQHRGQ
                     ESCGIVASDGEQLRSERAMGLVSEVFDEERLDRLPGAAAIGHTRYSTAGEVSIREAQP
                     FLVACQHGQVAVCHNGNLPFATESRKALERAGAIFSSTSDTEVILHGIARSHAATVPE
                     AIAEVLRETEGAFSMLFLTPDALVAVRDPRGFRPLALGRLGEAWVVASETCAFDLIDA
                     TYVRDVEPGEMIVITSEGLQSSFPLPARPHSFCVFEHVYFARPDSLVYNRSVNESRHK
                     MGRRLAVEQPADADIVVPVPDSGVAAAIGYAAESGIAFRFGLVRNHYIGRTFIEPKQS
                     IRSFGVRLKLNPVRDLIKGRRVVLIDDSIVRGTTSKKIVRMVREAGARAVHVRISCPP
                     TVSSCYYGVDTPDRRDLIAAQMPVAEVCRFIEADSLGYLSLEGMLAASGMGAESSCVA
                     CWTGRYPTRITREAETQYAREHEPVPSGDD"
     gene            2140..>3189
                     /locus_tag="AC159P_13190"
     CDS             2140..>3189
                     /locus_tag="AC159P_13190"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HIR3696447.1"
                     /translation="MAQSRRGCGCAVVGALALVAVFAAVAWMLFGTQLREWWQRPAPP
                     PASGKELQVRVLDVGQADSILIQAPSGKTVLVDAGEPGDGKRILEALKKYGAAQIDLL
                     VATHAHADHIGAADEVIKGATVGRVLYSGVPNTTKNYQDFLKAIDEKKLELIKAAPGQ
                     TFDLGDGVVLTVLAPSEPLFTKDELRSGGNEPNANSVVMRLDYGDFSMLLTGDAEAQT
                     EERLMRQGANVAADVLKVGHHGSKYATSENFVKRGQFKAALVSAGLDNRYGHPSQDAL
                     DRLRAAGVKLYRTDFQGEITLTSRGSDDFKITTAKEAKPEQDVWAGREALRDDSARRG
                     FIDFGDLPPAPTPKPE"
BASE COUNT          529 a         1123 c         1013 g          524 t
ORIGIN      
        1 aggccgttac tctgtctctc cgttccgtcc atcaacaagt gggtgaatgg tgaatggtga
       61 atagtaaatg gtcgaatact aaaagctgtt tctatttact attcaccatt caccattcac
      121 tacttcacgc catcatcccc ttctcgcgga gcgcctgcgt cagctcgtgg tcgctcggca
      181 ccgagagcac ctgcccggcc acgtcgagcg tgggcttgcg ctccttgccg tcgttgtgtt
      241 cgcgcaccca cgcggcggcg tccgcgtcct gctccacgtc cacgtactcg taggccacgc
      301 ccagcccgtc cagaaactcg cgcgtgtgcc gcgtgtcgcc gcaccagtcg gcgccgtaga
      361 ctttgaccgt taacacgaat gcgccccttt cccgagggag ataggctgac atcataacag
      421 aagtcagaag tcaggagtca gaagtcagaa tgaagaatcc tttatcttct gactcctgac
      481 ttcttcttca tcccctctcg cgcgggtgtt acaatgcgct ttcaagtttt ccccctttag
      541 agacacttcg ggcaccactg aatttcatga cggacggcgc aggcgagatg gagagagaca
      601 agttccgcga cgagtgcggc gtgttcggca tcttcggcaa cgcggaggcc gcgcggatga
      661 cctatctcgg cctctatcag ctccagcacc gcgggcagga gtcgtgcggc atcgtcgcct
      721 cggacggcga gcagttgcgt tccgagcgcg cgatggggct ggtctccgag gtcttcgacg
      781 aggagcggct ggatcgcttg ccgggcgcgg cggccatcgg ccacacgcgc tactcgaccg
      841 ccggggaggt ctcgatccgc gaggcgcagc ccttcctcgt cgcctgccag cacgggcagg
      901 tcgccgtctg ccacaacggc aacctgccgt tcgccaccga gtcgcgcaag gcactcgaac
      961 gcgcgggcgc catcttctcc tcgacctccg acaccgaagt catcctccac ggcatcgcgc
     1021 gctcgcacgc cgcgaccgtg cccgaggcca tcgcggaagt gctgcgcgag accgaaggcg
     1081 ccttctcgat gctcttcctc acgcccgacg ctctcgtcgc ggtgcgcgac ccgcgcggct
     1141 tcaggccgct cgcgctcggt cgtctgggcg aggcgtgggt cgtcgcctcc gagacgtgcg
     1201 cgttcgacct catcgacgcc acctatgtcc gcgacgtgga gccgggcgag atgatcgtca
     1261 tcacgagcga aggcttgcag tcttcgttcc cgctccccgc gcgcccgcac tcgttctgcg
     1321 tcttcgagca cgtctacttc gcgcgccccg actcgctcgt ctacaatcgc tcggtcaacg
     1381 agtcgcgcca caagatgggc cgccgcctcg ccgtcgagca gccggcggac gcggacatcg
     1441 tggtgcccgt gcccgactcg ggagtggccg ctgccatcgg ctacgccgcc gagagcggca
     1501 tcgccttccg cttcggcctc gtcaggaatc actacatcgg tcgcaccttc atcgagccga
     1561 agcagtcgat ccgcagcttc ggcgtgcggc tcaagctcaa ccccgtgcgc gacttgatca
     1621 agggccgccg cgtcgtcctg atcgacgact ccatcgtgcg cgggacgacc tcgaagaaga
     1681 tcgtgcggat ggtgcgcgag gcgggcgcgc gcgccgtcca cgtccgcatc agttgcccgc
     1741 ccacggtcag ttcgtgctac tacggcgtgg acacgcccga ccgccgcgac ctgatcgccg
     1801 cgcagatgcc ggtcgccgag gtctgccgct tcatcgaggc cgactcgctc ggctacctct
     1861 cgctcgaagg gatgctcgcc gcctccggca tgggagcgga atcctcctgc gtcgcctgct
     1921 ggacgggccg ttacccgacg cgcatcacgc gcgaggccga gacgcagtac gcgcgcgagc
     1981 acgaacccgt gccctccggc gacgactgag agcgccgccg cggtcgcacc tctcctcaag
     2041 ccgcaaaagt tcgcggcgta caaaccgtga ccgaggcttc atcaaactct tcaacgaagg
     2101 gcgcccgccg ggcgccgcga gaggggggga cgatttccgg tggcgcaaag tagacgcggg
     2161 tgcgggtgcg cggtggtggg ggcgttggcg ctggtcgccg tcttcgccgc ggtcgcgtgg
     2221 atgctcttcg ggacgcagct ccgcgagtgg tggcagcggc ccgcgccgcc gcccgcctcg
     2281 ggcaaggagc tgcaagtccg cgtgctcgac gtggggcagg ccgactcgat tctgattcag
     2341 gcgccgtcgg gcaagacggt gctcgtggac gcgggcgagc cgggcgacgg caagcgcatc
     2401 ctcgaagcgt tgaagaagta cggcgcggcg cagatcgacc tgctggtcgc cacgcacgcg
     2461 cacgccgacc acatcggcgc ggcggacgaa gtcatcaagg gcgcgaccgt cggcagagtc
     2521 ctctacagcg gcgtgccgaa cacgacgaag aactatcagg attttctcaa ggccatcgac
     2581 gagaagaagc tcgaactcat caaagccgcg ccgggccaga ccttcgacct cggcgacggc
     2641 gtggtgctca cggtgctcgc gccctcggag cctctgttca cgaaggacga gctgcgctcg
     2701 ggcggcaacg agccgaacgc gaactcggtc gtcatgcggc tcgactacgg cgacttctcg
     2761 atgctcctga cgggcgacgc cgaggcgcag accgaagagc ggctgatgcg gcagggcgcg
     2821 aacgtcgcgg cggacgtgct caaggtcggc caccacggct cgaagtacgc cacctcggag
     2881 aacttcgtca agcgcggcca gttcaaggcc gcgctcgtct ccgccggcct cgacaaccgc
     2941 tacggccacc cctcgcagga tgcgctcgac cgcctgcgcg ccgccggcgt caaactctac
     3001 cgcacggact ttcaaggcga gatcaccctc acctcgcgcg gctcggacga cttcaaaatc
     3061 acgaccgcga aagaggccaa gcccgaacag gacgtgtggg cgggccgcga ggcgctgcgc
     3121 gacgactcgg cccgccgcgg cttcatcgac ttcggcgacc tgccgcccgc ccccaccccg
     3181 aagcccgag
//
LOCUS       DBNGOH010000681         1595 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4315890, whole genome shotgun sequence.
ACCESSION   DBNGOH010000681 DBNGOH010000000
VERSION     DBNGOH010000681.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1595)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1595)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1595
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4315890"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(125..1387)
                     /locus_tag="AC159P_13195"
     CDS             complement(125..1387)
                     /locus_tag="AC159P_13195"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005007862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HIR3696448.1"
                     /translation="MMHTPRTAPLAVALLLFFACHATARQNGAFEFHEATFDAVGAAR
                     SPRLVAHRIKLAGGKSFDLNLPEGFGINVAAEGLRRVRFMAKSPDGRVFVTDMHSLAD
                     NKRGVVYVLDQFDAATRSFKKVTPYLKGLRNPNSLAFHRDRAGADWLYLALTDRLVRY
                     KYEAGSDAPTEKPEVLATFPDYGLNYKYGGWHLTRTVAVGGDGKIYVSVGSSCNACEE
                     KEEVRASIIEMDADGKNQRRYARGLRNAVGLRWVAGRLFATNMGADHLGDHRPADTMY
                     AVRDGANYGWPYCYQSGPKVLRDARFNARGAKLDCRKVPAAFAAFDSHSSPLGLEYFG
                     AEDGEQLGDSFLVALHGATKKNLGHGYRVARVAGDSRAPGPPEDFINGFLHAGKVHGR
                     PVDILSLGRGAFLLTDDYAGVVYYVYKK"
BASE COUNT          256 a          519 c          530 g          290 t
ORIGIN      
        1 cagagggcac ggaggaggca cggagggcac agagaagaca atccgttttc tctgtgccct
       61 ccgtgtcttc tccgtgcctt ctgtgttgaa atgaacccgc gtcacactca ggcagtatgc
      121 cggtctattt tttgtagacg taatagacga cacctgcgta gtcgtccgtg aggaggaagg
      181 cgccgcgccc gagacttaaa atatcgacgg gccgcccgtg gactttgccg gcgtgcagaa
      241 agccgttgat gaaatcttcc ggcgggccgg gcgcgcggct gtcgccggcg acgcgcgcga
      301 cgcggtagcc gtggccgagg ttcttcttcg tcgcgccgtg cagcgcgacg aggaatgaat
      361 cgcccaactg ctcgccgtct tcggcgccga aatactccag gccgaggggc gagctgtgcg
      421 agtcgaaggc cgcgaacgcc gcgggcactt tccggcagtc cagcttcgcg ccgcgcgcgt
      481 tgaaccgcgc gtcgcgcaag accttcgggc cggactggta gcagtagggc cacccgtaat
      541 tcgcgccgtc ccggacggcg tacatcgtgt ccgcgggccg gtggtcgccg aggtggtcgg
      601 cgcccatgtt ggtcgcgaac agacgccccg cgacccagcg caggcccacc gcgttgcgca
      661 ggccgcgcgc gtagcgtctt tgattcttgc cgtccgcgtc catctcgatg atcgaggccc
      721 gcacctcttc cttctcttcg caggcgttgc aactgctccc gaccgagacg taaatcttgc
      781 cgtcgccgcc gacggcgacc gtgcgcgtca ggtgccaccc gccgtacttg tagttcagcc
      841 cgtagtccgg gaaggtggcg agcacttcgg gcttctcggt cggcgcgtcg ctgcccgcct
      901 cgtatttgta gcgcacgagg cggtcggtca gcgccagata gagccagtcg gcgcccgcgc
      961 gatccctgtg gaaggcgagg ctgttcgggt tgcgcaagcc cttgaggtaa ggcgtgactt
     1021 tcttgaagct cctcgtcgcc gcgtcgaact ggtcgaggac gtagacgacg ccccgcttgt
     1081 tgtccgcgag gctgtgcatg tccgtgacga agacccgccc gtccggactc ttcgccatga
     1141 agcgcacgcg cctcagaccc tccgcggcga cgttgatgcc gaagccttcc ggcaggttga
     1201 ggtcgaacga cttgccgccc gcgagcttga tgcggtgtgc gaccagtcgc ggcgaacgcg
     1261 cggccccgac cgcatcgaag gtcgcttcat gaaactcgaa cgcgccgttc tgccgcgccg
     1321 tcgcgtggca ggcgaagaag agcaacaagg cgaccgcgag cggcgccgtg cgtggtgtgt
     1381 gcatcatgcg ggtttccaag tccgaagccg gtgtgtcatg ccgaacgcgg gcggcgttcc
     1441 gggtcatgtc gaagcagccg cgccgtccac ggcgtcggat tatatacaag cagccgcggc
     1501 gggctgcgag aatttatcgg aagcccgagt aatccccgga gcgccttcgc cccatcatag
     1561 gtgccgctgt tggattttcc gtacccgatg acacc
//
LOCUS       DBNGOH010000682         3958 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3583202, whole genome shotgun sequence.
ACCESSION   DBNGOH010000682 DBNGOH010000000
VERSION     DBNGOH010000682.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3958)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3958)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3958
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3583202"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..72
                     /locus_tag="AC159P_13200"
     CDS             <1..72
                     /locus_tag="AC159P_13200"
                     /inference="COORDINATES: protein motif:HMM:NF019265.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type sigma factor"
                     /protein_id="HIR3696449.1"
                     /translation="VSPKTVKRDWSVARAWLRREVGG"
     gene            80..2788
                     /locus_tag="AC159P_13205"
     CDS             80..2788
                     /locus_tag="AC159P_13205"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR3696450.1"
                     /translation="MTPERWQRVKELFEAALERGPAERAAFLAGACADDEEVGREVES
                     LLAAHERDSGFLNTPVVKLVADDGPMLAAGQRFGQYEAISLLGVGGMGEVYLAADTRL
                     GRKVALKLLPSSHTGDADRVRRFGQEARAASALNHPNIVTIHEIGEADSLHYIATEFV
                     EGVTLREHVAGGRMTMGEVLDVAAQVASALAAAHEVGIVHRDVKPENVMLRPDGFVKV
                     LDFGLAKLAPQQAAPAPQAATVLDVKTNPGVVMGTVAYMSPEQARGLEVDARTDIWSL
                     GVVLYEMFSGRAPFTGETPSHILVSIMERVPPPLALDAEAPAELERIISKALAKDREE
                     RYRTAGEMALELKSLKEELEVAARLKRRPPPHANGKEYAARGDGFRAAGGFGEPAART
                     GHVIPPRSTASVESVVGGIKRHKTYAAAALLVLLVGAVGLTYFAFNRYQSDPGVRGRK
                     SIAVLPLEPIDAARRDEMYELGIADSLIHRLSLMKGLIVRPLSATRKYADREQDPLAA
                     GKEQRVDYVLASNYQLADGKIRVTAQLLNVASGQIEETTLVEREAGNVFTAQDAVAGE
                     VVRLLRTRAVNTSDVPAAKRRTTSEEAYRLYLQGRNLSMKRGAENHKRAIEYFERAIR
                     LDPNYAAAYAGIARSVLYTGLGGEEVKTGGGEVREYVDKALALDNDLAEAYVARADSA
                     LLAEWDFPAVERDLLRAIELEPNNDAAHWLYALLLAYRGRFDEALAEIETAQAIDPSA
                     LVYMRDRGRILYYARRYDEAIAEYERAIELGAPDMIGGWLRFAYAMKGDEARAYEIFI
                     NAQRRNNPDLVAIYEQTYAAEGYRGVRRKLLENFKLNNNLPGAGFYLMAGVATGLGEE
                     DLAFEYLNRAVERREWRVVMMEVDPPLDPVRDDPRFAEVVRRAGLQ"
     gene            2861..>3958
                     /locus_tag="AC159P_13210"
     CDS             2861..>3958
                     /locus_tag="AC159P_13210"
                     /inference="COORDINATES: protein motif:HMM:NF025006.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HIR3696451.1"
                     /translation="MKLATILFVVLASFAAARAQAPTGTVAGVVTDSAGAPVAAARLR
                     LTHRDTGLTRSLTTSDEGDYSAAALPPGLYLLTAEAEGFRLLERAATVETGATTTLNL
                     TLEVGSIDEKVTVDAAAPLIQYEHHQVGGLVSRNQIEGLPLNGRSFLELAKLEPGVQP
                     PTRVASNRSFVPALGQPVGTNGRGTRVTVDGGSVMAVGSGGTAMNFSQEVVQEFQVAT
                     VNFDLTTGLTSGAAVNVTTRAGGNELHGTGFYFLRDHTLSAYPALGRDPVNPDPFFQR
                     RQFGFAVGGPVRRDRLFFFGAYERNEQRGVAATTILTPEFAHLNRVTPSPFYNTQLSL
                     RLDARLTQAHTAFARYAHDRISAYGPPNQPSA"
BASE COUNT          678 a         1355 c         1381 g          544 t
ORIGIN      
        1 gtctcgccga agacggtcaa gcgcgactgg agcgtggcgc gggcgtggct gcggcgcgag
       61 gtcggcgggt aagacgaaga tgactcccga acgttggcaa cgtgtgaaag agctgttcga
      121 ggcggcgctg gaacgcggcc cggcggagcg cgccgcgttt ctcgcgggcg cctgcgcgga
      181 cgacgaagag gtcgggcgcg aggtcgagtc gctgctcgcg gcgcacgagc gcgactccgg
      241 ctttctgaac acgcccgtcg tgaagctcgt cgccgacgac ggccccatgc tggcggcggg
      301 acagcgcttc gggcagtacg aagcgatctc gctgctcggc gtgggcggga tgggcgaggt
      361 gtacctggcg gcagacacac ggctggggcg caaggtcgcg ctcaagctgc tgccctcttc
      421 gcacacgggc gacgccgacc gcgtgcggcg cttcgggcag gaggcgcgcg ccgcctcggc
      481 cctgaaccac ccgaacatcg tcaccatcca cgagatcggc gaggccgact cgctccacta
      541 catcgccacc gagttcgtcg agggcgtgac gctgcgcgag cacgtggcgg gcgggcggat
      601 gacgatgggc gaagtgctcg acgtcgccgc ccaggtcgcc tccgcgctcg cggcggcgca
      661 cgaggtcggc atcgtccacc gcgacgtcaa gccggagaac gtcatgctgc ggcccgacgg
      721 cttcgtcaag gtcctggact tcggcctcgc caagctcgcg cctcagcaag cggcacccgc
      781 gccgcaggcg gcgaccgtct tggatgtcaa gaccaacccc ggcgtcgtga tgggcaccgt
      841 ggcctacatg tcgccggagc aggcgcgcgg gctggaggtg gacgcgcgca cggacatctg
      901 gtctctgggc gtggtcctct atgaaatgtt ttcggggcgc gcgccgttca cgggcgagac
      961 gccgagccac atactcgtct cgatcatgga gcgcgtgccg ccgccgctcg cgctcgacgc
     1021 cgaggcgccc gccgagctgg agcggatcat ctcgaaggcg ctcgccaaag accgcgagga
     1081 gcgttaccgg acggcgggcg agatggcgct cgagctgaag agcctgaagg aagagctgga
     1141 ggtcgcggcg cggctcaaac gccggcctcc gccgcacgcg aacgggaaag agtacgccgc
     1201 acggggcgat gggttccggg cagcgggggg cttcggcgaa ccggcggcgc gcaccggcca
     1261 cgtcatcccg ccgcgctcga cggcgagcgt cgagtccgtg gtcggcggga tcaaacggca
     1321 caagacttac gccgccgccg cactgctcgt tcttctcgtc ggggccgtcg gcctgacgta
     1381 cttcgccttc aaccggtacc agtccgaccc gggcgtgcgc ggcaggaaat caatcgccgt
     1441 ccttccgctc gaacccatcg acgcggcgcg gcgcgacgag atgtacgaac tcggcatcgc
     1501 cgattctctg attcaccggc tgagcctgat gaaagggttg atcgttcgcc cgctgagcgc
     1561 cacgcgcaaa tacgccgacc gcgaacaaga cccgctcgcc gcggggaagg aacagcgggt
     1621 cgattacgtc ctcgcctcga actaccagtt ggccgacggc aagatccgcg tcaccgcgca
     1681 gctcctgaac gtggcgagcg ggcagatcga agagacgacg ctcgtcgaga gagaggcggg
     1741 caacgtcttc acggcgcagg acgcggtcgc gggcgaggtc gtcagactgt tgcggacgcg
     1801 cgccgtcaac acctcggacg tcccggcggc gaagcgccgg acgaccagcg aagaggcgta
     1861 caggctctac ctgcaaggca gaaatctgtc gatgaagcgg ggggcggaga atcacaaaag
     1921 ggcgatcgaa tacttcgagc gggccatccg gctcgacccg aattacgccg cggcctacgc
     1981 cgggatagcc cgctccgtcc tttacacggg cctcggcggc gaggaagtca aaaccggcgg
     2041 cggggaggtc agggagtacg tcgacaaggc gctggcgctc gacaacgatc tggccgaagc
     2101 ctacgtggcg cgggccgatt cagcactttt agccgaatgg gacttccccg cggtggagag
     2161 agacctgctc cgcgccatcg agctggagcc gaacaacgac gcggcgcact ggctctacgc
     2221 ccttctcctg gcctaccgcg ggcggttcga cgaggcgctc gccgagatcg agaccgcgca
     2281 ggccatcgac cccagcgcgc tcgtctacat gagagaccgc gggcgcatcc tgtactacgc
     2341 ccgccgctac gacgaggcga tcgccgagta tgaacgggcg atcgagctgg gcgcgcccga
     2401 catgatcggc ggctggctcc ggttcgccta cgcgatgaag ggcgacgagg cgcgcgcgta
     2461 cgagatcttt atcaacgccc agagacgaaa taatcccgac ctcgtcgcca tatatgaaca
     2521 gacttacgcc gcggaaggct atcggggggt gaggcggaag ctgctggaga acttcaagct
     2581 caacaacaac ctgcccgggg cggggtttta cttgatggcg ggggtggcca ccgggctggg
     2641 agaagaggat ttggcgttcg agtacctgaa cagggccgtc gagaggcgcg agtggcgcgt
     2701 cgtcatgatg gaggttgacc cgcccctcga ccccgtgcgc gacgacccgc gcttcgccga
     2761 ggtggtcagg cgcgccgggc tgcagtaaag acgaggcgaa ggaagtttga agaggccggg
     2821 gcgcaaccgt accggtcggg gcgagcgagg cgagataccc ttgaagctgg cgacaatttt
     2881 attcgtcgtg ttggcctcgt tcgccgcggc ccgcgcgcag gcgccgaccg gcaccgtcgc
     2941 gggcgtcgtc acagactccg ccggcgcgcc cgtcgccgcc gcgcggctgc gtctcacaca
     3001 ccgcgacacc gggctgacgc gcagcctcac cacttcggac gaaggcgact acagtgccgc
     3061 ggcgctgccg cccggcctct acctcttgac cgcggaggcc gagggcttcc gcctgctcga
     3121 acgcgcggcc acggtcgaga ccggcgcgac caccacgctc aacctcacgc tcgaagtcgg
     3181 cagcatcgac gagaaggtga cggtggacgc cgccgcgccg ctcatccagt acgagcacca
     3241 tcaggtcggg ggactcgtca gccgcaacca gatcgagggc ctgccgctca acgggcgcag
     3301 ctttctggag ctggccaagc tggagccggg cgtccagccc cccacgcgcg tcgccagcaa
     3361 ccgcagcttc gtgccggcgc tcggccagcc cgtcggcacc aacgggcgcg gcacgcgcgt
     3421 caccgtggac ggcggcagcg tcatggcggt cggcagcggc ggcacggcga tgaacttttc
     3481 gcaggaggtg gtgcaggagt tccaggtcgc gaccgtcaac ttcgacctca cgaccggcct
     3541 caccagcggc gccgccgtca acgtcaccac gcgcgccggc ggcaacgagc tgcacggcac
     3601 cggcttctac ttcctgcgcg accacactct ttcggcctat cccgcgctgg gccgcgaccc
     3661 cgtaaacccg gacccgttct tccagcgccg tcagttcggg ttcgccgtgg gcggcccggt
     3721 gcggcgcgac cgcctcttct tcttcggcgc ctacgagcgc aacgagcagc gcggggtggc
     3781 ggcgacgacg atcttaacgc cggagttcgc gcacctgaac cgcgtcacac ccagcccgtt
     3841 ctacaacacc cagctcagcc tgcggctgga cgcgcggctg acgcaggcgc acaccgcctt
     3901 cgcacgctac gcgcacgacc gtatcagcgc ctacggcccg cccaaccagc cgagcgcg
//
LOCUS       DBNGOH010000683         4099 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4683030, whole genome shotgun sequence.
ACCESSION   DBNGOH010000683 DBNGOH010000000
VERSION     DBNGOH010000683.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4099)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4099)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4099
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4683030"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="AC159P_13215"
     CDS             <1..250
                     /locus_tag="AC159P_13215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HIR3696452.1"
                     /translation="NYEEGAKGKHTPVVRNYVVEKLKSGKSKHALDVQGFADAPVFNL
                     RLEDCVFENAAQPSIVKNVRGLALKNVRVNGKVWESEG"
     gene            464..718
                     /locus_tag="AC159P_13220"
     CDS             464..718
                     /locus_tag="AC159P_13220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696453.1"
                     /translation="MASTSKRSAKVICHEGNVWVSPRQFWNWVRDGVVEYVSEPPLTG
                     RFQGQREQFLVSVGHTLLNITCPEHLHAVLQARHRLRKQK"
     gene            771..1478
                     /locus_tag="AC159P_13225"
     CDS             771..1478
                     /locus_tag="AC159P_13225"
                     /inference="COORDINATES: protein motif:HMM:NF019349.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIR3696454.1"
                     /translation="MLKLIQPISKKNQVVLTIKEAILSGSIAPGAQIVESRIAQQLGT
                     GIPLVREALIELEHQGFVQKTPYKGTTVTELGPKEIRQMFQLRGELEALAVVWAKENA
                     TAADIEELRALIAKMEATASGRDSEKFCEADLAFHRKLWELARNPYLADALERVVLPL
                     FAFSVMRIRRERNGFAESAAQHARIVEALPEMSAGYLRDLMKELVGCWKDEMLEPLLS
                     KDGAGEDEGGASAGPRP"
     gene            1645..3837
                     /locus_tag="AC159P_13230"
     CDS             1645..3837
                     /locus_tag="AC159P_13230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional rhamnulose-1-phosphate
                     aldolase/short-chain dehydrogenase"
                     /protein_id="HIR3696455.1"
                     /translation="MSKEKRALSFVKYLWQDEVADQLSGLDRLVYRSNKLGEDLTLTN
                     TGGGNTSSKLVERDPLTGAEVEVLWVKGSGGDLRTAKRDGFASFYLDKIRAMKPIYTN
                     HPENGPKTEIEDAMYPMYSHCVFDLNPRAGSIDTPLHTLVPYTHVDHLHPNAVIAVAA
                     SVNQEKLTKEIYGDEIIYIPWQRPGFDIGLKIEQLIAENPQAKGILLGHHGMSSWNND
                     DKTCYETALEIIDRAARYIEEHDKGEKTFGGPKHESIEETERRRIIAEVIPFLRGQVS
                     SNKRFVGTVQDDERMLRFVNSHDGPRLAELGTSCPDHFLRTKIKPFFVDWDPATRDIE
                     ALKAAIAEGIVEYRKDYAAYYEKCKHPDSPAMRDPNPTVVLIPGLGMVAWGKNKSESR
                     VTGEFYNCAIEVMRGAEAMDEYEAMDQQEAFDIEYWLLEEAKLRRMPPEKELDRRVVV
                     IIGAGAGIGKATAHRVVKEGAHVVCVDLDEASAKETAQEIIDKYGPGIGVAGTGISNC
                     GPAIGLACDITNRESVAKMFEDVMLAYGGIDDVIVTAGIFVPPDKSGRVEDRQWALTY
                     AINVTGPYVVADEAYKIFKKQGLTGNLVLTTSANAVVAKKGSLAYDTSKSAANHLARE
                     LAIEMAPLVRVNAVAPATVVKGSTMFPRDRVIASLTKYNIEHDEDEETEALRDKLAQF
                     YAKRSLTQTPIEPDDQAEALFLLISPRLSKTTGHVIPVDGGLQDGFLR"
BASE COUNT          834 a         1302 c         1350 g          613 t
ORIGIN      
        1 caactacgag gagggcgcga agggcaagca cacgcccgtc gtgcgcaact atgtcgtcga
       61 gaagctgaag agcggcaaga gcaagcacgc gctcgacgtg cagggcttcg ccgacgcgcc
      121 cgtcttcaac ctgcgtctgg aagactgcgt cttcgagaac gcggcgcagc cgagcatcgt
      181 caagaacgtc cgcggcctcg cgctgaaaaa cgtccgcgtc aacgggaagg tctgggaaag
      241 tgagggctga aaccgcccgc gccgcgtccg caggcgtctg aaaagtgtgt gcgaaaactc
      301 gcagaaatcc ggggaaaaac gcggaaagcc gtgcattggc gtatgaagat catattataa
      361 tcttgcaatt tgttgggagt tgagctatta actttcgcat acccggccag catcagcacg
      421 cattcgaatc cgaggggttg aaagtgaggg ccttcgtctc tctatggcga gcaccagcaa
      481 acgatccgcg aaagtcatct gccacgaggg gaacgtctgg gtctcgccca ggcagttctg
      541 gaactgggtg cgcgacgggg tggtcgagta cgtgagcgag ccgccgctga cgggcaggtt
      601 ccagggtcag cgcgagcagt tcctggtcag cgtcgggcac acgctgctca acatcacctg
      661 cccagagcac ctgcacgcgg tcttgcaggc gcggcaccgc ctgcgcaagc agaagtgaga
      721 cgggcgccgc ggcggcctcg cgcgcggcgc agtcacggag cgcgataggc atgctgaaac
      781 tgattcagcc tatctcgaag aagaaccagg tcgtcctgac gatcaaggag gcgatcctgt
      841 ccggctccat cgcccccggc gcgcagatcg tcgagagccg catcgcgcag cagctcggca
      901 ccggcatccc gctcgtcaga gaggcgctga tcgaactcga acatcagggc ttcgtgcaga
      961 agacgccgta caaggggacg acggtgacgg agctggggcc gaaagagatt cggcagatgt
     1021 tccagttgcg cggcgagctg gaggcgctgg ccgtcgtgtg ggcgaaggag aacgccacgg
     1081 cggcagacat cgaagagctg cgcgcgctca tcgccaagat ggaggccaca gcgtccgggc
     1141 gcgactccga aaagttctgc gaggccgacc tcgccttcca ccgcaagctg tgggagctgg
     1201 cgcgcaaccc gtatctggcg gacgcgctcg aacgcgtggt gctgccgctc ttcgccttct
     1261 cggtgatgag gatccggcgc gagcgcaatg gctttgccga gagcgcggcg cagcacgcgc
     1321 ggatcgtcga ggcgctgccg gagatgagcg cgggctacct gcgcgacctg atgaaggagc
     1381 tggtcggctg ctggaaggac gagatgctgg agccgctcct ctccaaggac ggcgcgggcg
     1441 aagacgaagg cggcgcttca gccggtcccc gcccgtgaac gggcaagaac ttttcaggcg
     1501 gggccgcgcc gccgccccgc cgcagtttca acgaacagtc aacggacgcc gcggccccct
     1561 cgcgagtcaa cagtctggtc atccgtcagc atcacctttt gaggggatgg gcttgcagca
     1621 agccccatct gccgtggagg tctcatgagc aaagagaaaa gggctttgag tttcgtcaag
     1681 tacctgtggc aggacgaggt cgccgaccag ctttcgggac tcgaccgtct ggtctaccgc
     1741 tcgaacaaac tcggcgaaga cctgacgctc accaacaccg ggggcggcaa cacctcctcg
     1801 aagctcgtcg agcgcgaccc gctcacgggc gcggaggtcg aggtgttgtg ggtcaagggg
     1861 tcgggcggcg atttgcgcac ggccaagcgc gacggcttcg cgtcgttcta cctcgacaag
     1921 atacgcgcga tgaagccgat ctacacgaac cacccggaga acggcccgaa gaccgagatc
     1981 gaggacgcga tgtacccgat gtacagccac tgcgtcttcg acctcaaccc gcgcgccggc
     2041 tccatcgaca cgccgctcca cacgctcgtc ccctacacgc acgtcgatca cctgcacccg
     2101 aacgcggtca tcgccgtcgc cgcctcggtc aaccaggaga agctcacgaa agagatttac
     2161 ggcgacgaga tcatctacat cccgtggcag cgaccgggct tcgacatcgg gctgaagatc
     2221 gagcagctca tcgccgagaa cccgcaggcc aagggcatcc tgctcgggca ccacgggatg
     2281 agttcgtgga acaacgacga caagacctgc tacgagacgg cgctggagat catcgaccgc
     2341 gccgcgcgct acatcgagga gcacgacaag ggcgagaaga ccttcggcgg gccgaagcac
     2401 gagtcgatcg aggagaccga gcgccgccgc atcatcgccg aggtcatccc tttcttgcgc
     2461 gggcaggtct cctcgaacaa gcgcttcgtc ggtaccgtgc aggacgacga gcggatgctc
     2521 cgcttcgtca acagccacga cggcccgcgc ctcgccgagc tgggcacgtc ctgccccgac
     2581 cacttcctgc gcaccaagat caagcccttc ttcgtcgatt gggacccggc gacgcgcgac
     2641 atcgaggcgc tgaaagcagc catcgccgaa ggcatcgtcg agtaccgcaa ggactacgcc
     2701 gcctactacg agaagtgcaa gcacccggac tcgcccgcga tgcgcgaccc gaacccgacg
     2761 gtggtgctga tccccggcct cggcatggtc gcgtggggca agaacaagag cgagtcgcgc
     2821 gtgacgggcg agttctacaa ctgcgccatc gaggtgatgc gcggcgccga ggcgatggac
     2881 gagtacgagg cgatggatca gcaggaggcg ttcgacatcg agtactggct gctcgaagag
     2941 gcgaagctgc ggcggatgcc gcccgagaag gaactcgacc gccgcgtcgt cgtcatcatc
     3001 ggcgcgggcg ccggcatcgg caaggcgacc gcccaccgcg tcgtcaaaga gggcgcgcac
     3061 gtcgtctgcg ttgaccttga tgaagcgtcg gccaaagaga cggcgcagga gatcatcgac
     3121 aagtacggcc ccggcatcgg cgtcgcgggc acaggcatca gcaactgcgg cccggccatc
     3181 ggtctggcct gcgacatcac gaaccgcgag tcggtcgcca agatgttcga ggacgtgatg
     3241 ctcgcctacg gcggcatcga cgatgtcatc gtcaccgcgg gcatcttcgt cccgcccgac
     3301 aagtcggggc gcgtcgaaga ccggcagtgg gccttgacct acgccatcaa cgtcacaggc
     3361 ccctacgtcg tcgccgacga ggcgtacaag attttcaaga agcagggact gaccggcaac
     3421 ctcgtcctga cgacgagcgc caacgcggtg gtggcgaaga aagggagcct cgcctacgac
     3481 accagcaagt cggcggccaa ccacctcgcg cgcgagctgg ccatcgagat ggcgccgctc
     3541 gtgcgcgtca acgccgtcgc gcccgccacg gtcgtcaaag ggagcacgat gttcccgcgc
     3601 gaccgcgtca tcgcttcctt gacgaagtac aatatcgagc acgacgagga cgaggagacc
     3661 gaggcgctca gggacaagct ggcgcagttc tacgccaagc gctcgctgac gcagacgccc
     3721 atcgagccgg acgatcaggc cgaggcgctg ttcctgctga tctcgccgcg cctgtcgaag
     3781 acgacggggc acgtcatccc cgtggacggc gggttgcagg acgggttctt gagatagaag
     3841 gcgcggattt ccgcacacga ttcccggctc cgccgcctga tgtgcggtga ggcttcgcct
     3901 caccggcgac gtgcttcttc tgtcttgctg aggctccgcc tcagcgcggg ggcgtagccc
     3961 cggaataaaa aacgcgcgcc cgccgagagg gcatagccct cccgtacatc agacggcgga
     4021 gccgcaagaa gataatttgg ggaagcggtc tgatcttcta gtggtatcgt ccgtgggagc
     4081 aatgacattt cggaggtga
//
LOCUS       DBNGOH010000684         9521 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_833834, whole genome shotgun sequence.
ACCESSION   DBNGOH010000684 DBNGOH010000000
VERSION     DBNGOH010000684.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 9521)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9521)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9521
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_833834"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1079)
                     /locus_tag="AC159P_13235"
     CDS             complement(<1..1079)
                     /locus_tag="AC159P_13235"
                     /inference="COORDINATES: protein motif:HMM:NF051698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HIR3696456.1"
                     /translation="MSSLRNRVSALAVALVVLLLGGEALAQSVTGNVSGTVTDSSGAI
                     VPGAAVTLIGEQTGETRNLTTNDDGRFNFAAVQPGVYKVKVELQGFQTLLQERVILSA
                     NENLALGELKLQPGQVTETVTVASAGAVVETESSDLTARLTADQINLISTKGRDVTSL
                     LRLLPGTSNNDDIEAVGEGFGTDLPNISGQRGRSTVSTVDGLNASEPSGSNKLSMTIN
                     QDAVAEVKVLRNNYGAEYGNNGGALINLVSKGGGREYAGTVYYFLRNEALNASPFFNN
                     KAGLDRPLYRHRIWGGNFGGPLWIPKFGEGGSHWLKDKAFFFYSLEKPHQITPQDPRF
                     VTVPTALERDGDFSQSINSSNNAVFV"
     gene            1555..2442
                     /locus_tag="AC159P_13240"
     CDS             1555..2442
                     /locus_tag="AC159P_13240"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR3696457.1"
                     /translation="MASSRYVDRVTIITGGSSGIGKGCAHEFVRAGAKVVICCNNERE
                     GTAVAEDLHAVARKQGAGDISFVYCDVRSAEDLRRLVEATLSLHGRIDCLVNNAGWHP
                     PHKAIDDFSVAEFRELLELNLVSVFTACKLALPALRETRGNIINMSSLVGSIGQLHAT
                     TYVATKGAVTAFTKALAIDEAAHGVRVNSVSPSNIYTPLWQEAIALAPDPERCRLEGE
                     EAQLMGRMGGPEEVGRLCLFIAAEATFTTGVDHVISGGAELGYGRKSRSEQHALSAEA
                     LDEALVTASSSFSPNGRRT"
     gene            complement(2399..3469)
                     /locus_tag="AC159P_13245"
     CDS             complement(2399..3469)
                     /locus_tag="AC159P_13245"
                     /inference="COORDINATES: protein motif:HMM:NF012742.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIR3696458.1"
                     /translation="MPVTLADIARELGVSKMTVSRAINNHPSINADTRERVLEVARRL
                     NYQPNQHARALATNRSYLIGVVVPDLMHSYFAEICRGVEDFARPSGYRNLICNTDEDP
                     AKELDETGALLPRTDGLIVASSLPPSDAGFYRRLVKDGAKVVFIDRRLDGVRCPAVTT
                     DDVQVGMLATEHLVGLGHRRIGHLRGTGASTAAGRFEGYRRALEKHGIALAAGDVRDC
                     GFTEREGYEAMRGWLAEGDVPGAIFAVNDPAAIGAMQALGEAGLRVGRDVALVGGGNI
                     HYADMLRVPLTTVSWSRSEMGQQAARLLLQLIDEKEPPTKKPEHVVVPPELIVRSSSG
                     AGQAAKASVKSAARSARSSTRR"
     gene            3659..3901
                     /locus_tag="AC159P_13250"
     CDS             3659..3901
                     /locus_tag="AC159P_13250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696459.1"
                     /translation="MGQKYQVRIIKRGQRESAESVVEAATPERAERAEPSERELKTVM
                     SGWVREHRQRTEEYRRSFAGLLRQVGFNPLPAGRRS"
     gene            complement(4041..6161)
                     /gene="eutB"
                     /locus_tag="AC159P_13255"
     CDS             complement(4041..6161)
                     /gene="eutB"
                     /locus_tag="AC159P_13255"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF018461.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008851 - ethanolamine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutB"
                     /protein_id="HIR3696460.1"
                     /translation="MNGDELRQIFALAGEFKEGDLAVGGTRDEGVRGEARRSLAALRL
                     GDIARTALVEDGVSDALAGALDARLASDISGLTVGGLKGVLLGAGGAEWVGRHREGLA
                     SECIAAVAKLMTNDELSTVARSLFNPLPEANGGVCIGSRGHFGSRIQPNSPGDEEEEI
                     LFSVLEGLSYGCGDVVLGINPASDDVETIIRLEELLRRVVERLELPTRYCVLSDIVKQ
                     SEARARVKVDVGFQSLAGTSKALGGMVGLDVEGVLELARGFGGLYFETGQGSAVTNNA
                     AEGVDMLTLEARAYGVARHIRRRTGAWMIVNDVAGFIGPEVFRTGEQLERACLEDAVM
                     AKLHGLTMGLDVCSTFHMGVEPAQLARLTERIVERAAPAYLMAVAGNADPMLGYLTTS
                     FREHPALRRRTRRGVAAPMRRRLEALGVLDAGGDVLPDAPGPAALYAVYMKAGGDTRT
                     EEELRAEGEKKLADVRGRGFDLGHGHGADFAAPREVETRLDAIYRHARRALYATLDEA
                     VVREASPRALRVRTLARDREEFLAHPPSGERLLDEDAARIRDLYSTRRPQVQFVVSDG
                     LNADALNENLRGVLPRLRAALSEAGCHVGEADVVVRNGRVRAGYHVGALLGVEVVVHL
                     IGERPGTGLDTLSVYMTYGRDAAGRPRWSPDMDHAFTNAVCGVHPRGKRPEVAAEEVA
                     RYVLRMLEEKRSGVALGPAYGLRR"
     gene            6662..7573
                     /locus_tag="AC159P_13260"
     CDS             6662..7573
                     /locus_tag="AC159P_13260"
                     /inference="COORDINATES: protein motif:HMM:NF013618.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008933 - peptidoglycan lytic
                     transglycosylase activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HIR3696461.1"
                     /translation="MTKIKSILFALSAALLFIAADSPAGAQTAKKKKTAAQPQQAAAA
                     EVASDPESFKKDYIKAAEEYEASLRELAASYEGGLKKLSEKHEALKGLYTDGLIARRE
                     FEESEKALAEAGAKVEDVRREIASVEKTLEVARRPTPAVAPDTAALPRVEVAWTTGNA
                     KIDGLIRLNGQRYGVDPYLIYCIMHQESRFGAGATSYKGAMGLMQLMPGTAARFGVTN
                     PYDPAQSIMGGTRYLKELLRLFGGRVDLALAGYNAGEGAVMKYGRRVPPYRETQDYVR
                     IIGTRYSKGAAVPTAKVTERKAGGAGK"
     gene            complement(7674..9350)
                     /locus_tag="AC159P_13265"
     CDS             complement(7674..9350)
                     /locus_tag="AC159P_13265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HIR3696462.1"
                     /translation="MKRGMAVVLAFVCVWCALPASGQDAAAARQERINAEVFWKIRRE
                     GTENSQIMRTLHFLTDVYGPRLTGSPNHKAAADWAARQMETWGLRNARLEPWDFGHPG
                     WQNERLSVHLVAPVKDSLVCEALAWTPGTSGPVRARAVQIVVPERPTRQQLDFYFATV
                     KEQVRGKIVLVGKHTPLPVSFNPPFKRRDEQEARNQFDPNNPTPSPFAAAPTPSPTPA
                     PNQPQPLTNQQLAEAVDRFLVESGARVRVNDAGREHGQIRAFNNRTFDVSKAVPTAVC
                     RNEDYGRITRILADSTPVDLEFDIVNRSYPEGRTSYNVIAEIPGDEDKDEIVMLGGHL
                     DSWHAATGATDNAIGCAIMMEAARILRVVGAKPRRTIRVALWSGEEQGLLGSQAYVKK
                     YFGSFEKPQPGHAKFNGYFNIDSGTGRVRGATVFGPPEAAGILRQALAPFADLGVEGA
                     IATRSRNLGGSDHTSFNNAGLPGIGMGQDPIEYGTHTWHTNLDTYERIVEDDVKAAAI
                     VVAAAAYHLATRDEMLPRFNRERMPAPPPTPTPTPTPAPTAPPPSTTAAR"
BASE COUNT         1493 a         3203 c         3240 g         1585 t
ORIGIN      
        1 acgaagaccg cgttgttcga ggagttgatc gactgcgaga agtcgccgtc gcgttcgagc
       61 gcggtcggca ccgtcacgaa gcgcgggtct tgcggcgtga tctggtgcgg cttctccaga
      121 gagtagaaga agaacgcctt gtccttgagc cagtgcgagc cgccctcgcc gaacttcgga
      181 atccacaaag ggccgccgaa gttgccgccc cagatgcggt ggcggtagag cgggcggtcg
      241 aggcccgcct tgttgttgaa gaaggggctg gcgttcagag cctcgttgcg gaggaagtag
      301 tagacggtgc ccgcgtactc gcgcccgccg cccttcgaga cgaggttgat gagcgcgccg
      361 ccgttgttgc cgtactcggc gccgtagttg ttgcgcagca ccttgacctc ggccaccgcg
      421 tcctggttga tcgtcatgct cagtttgttc gacccgctcg gctcgctggc gttgaggccg
      481 tccaccgtgg agaccgtgga gcgcccgcgc tggcccgaga tgttcggcag gtctgtgccg
      541 aagccctcgc cgacggcctc gatgtcgtcg ttgttcgaag tgccgggcag cagccggagg
      601 agcgaggtca cgtcgcgccc cttggtcgag atgaggttga tctggtctgc ggtgagccgc
      661 gccgtcagat cgctcgactc ggtctcgacc accgccccgg cgctcgccac cgtcaccgtc
      721 tccgtcacct ggccgggctg caacttcaac tcgccgagcg cgaggttctc gttggccgag
      781 aggatgacgc gctcttgaag gagcgtctgg aagccctgta gctcgacctt gaccttgtag
      841 acgccgggct gcaccgccgc gaagttgaac ctgccgtcgt cgttcgtcgt caggttgcgc
      901 gtctcccccg tctgctcgcc gatgagcgtg acggcggcgc ccggcacgat ggcgccggac
      961 gaatccgtca ccgtgcccga gacattgccc gtcacgctct gggccaatgc ctcgccgccg
     1021 agcagcaaaa caacgagcgc caccgccagc gccgaaaccc gattcctgag ggaagacatc
     1081 ttaagctctc ctttcacgaa actccgggac ggcctcgccg ccctcgctac gaacatcgcg
     1141 tgcccgtcgc cgcttaccgc gcgtaccttc accgacgaga agcgtgcgca cgcctctggc
     1201 cgtcgcccgc ccgcgaattt tcattttggt ctcgacgaac ttccgacccg gacgtaaccg
     1261 cccaacgcac acaccgcgat ggcggcggcc cttcggtctt cgcgcgcgac cgccggcacg
     1321 ccttcgccta ccctttcgcc agattcgaac tgcgcataaa tgttgtgtgc tcgctggttc
     1381 gccgcgccgt tgaaagaagc ttcatcgcgg ttcaaggccg cggcggcccg taatagttga
     1441 aacactatac ttgattttgg taccgataac aagtattatt ttaccggtaa cagttcggag
     1501 gggcggcacg cgtccggttc gcggcaacga aggccgacca ctggaggtca tggaatggct
     1561 agctcgcggt acgttgacag ggtgacgatc atcaccggcg gcagcagcgg catcggcaag
     1621 ggctgtgcgc acgagttcgt ccgcgccggg gcgaaggtcg tcatctgctg caacaacgag
     1681 cgggagggca cggcggtcgc cgaagatttg cacgcggtcg cgcgcaagca aggcgcgggc
     1741 gacatctcct tcgtctactg cgacgtgcgg agcgcggaag acctgcggcg cctcgtcgag
     1801 gccacgctct cgctccacgg gaggatcgac tgcctcgtca acaacgcggg ctggcacccg
     1861 ccgcacaagg caatcgacga cttctccgtc gcggagttcc gcgagctgtt ggagctgaac
     1921 ctcgtcagcg tcttcaccgc ctgcaagctg gcgctgcccg ccttgcgcga gacgcgcggc
     1981 aacatcatca acatgtcgag cctcgtgggc agcatcggtc agcttcacgc gacgacgtat
     2041 gtggcgacga agggcgcggt gacggccttc acgaaggcgc tggcgatcga cgaggcggcg
     2101 cacggcgtca gggtgaactc ggtgtcgccg agcaacatct acacgccgct gtggcaggag
     2161 gcgattgcgc tggctccgga cccggagcgg tgtcgtctgg agggcgagga ggcgcagttg
     2221 atgggtcgga tgggggggcc ggaggaggtc ggtcggctgt gcctgttcat cgcggcggag
     2281 gcgacgttca cgacgggcgt cgatcacgtc atctcgggcg gggcggagtt gggctacggg
     2341 cggaagagcc gttccgagca gcacgccctg tcggctgagg cgctggacga agcgctcgtc
     2401 accgcctcgt cgagcttctc gccgaacggg cggcggactt gacggacgcc ttcgccgcct
     2461 gtcccgcgcc ggacgagctg cggacgatca gttccggcgg gacgacgacg tgctccggct
     2521 ttttcgtcgg cggctctttc tcgtcgatga gttgcagcag caggcgcgcg gcctgttgtc
     2581 ccatctcgct gcgcgaccag gagacggtcg tcagcggcac acgcagcatg tccgcgtagt
     2641 ggatgttgcc gccgcccacg agcgccacgt cgcgcccgac gcgcaggccc gcctcgccga
     2701 gcgcctgcat cgcgccgatg gccgccgggt cgttgacggc gaagatcgcg cccggcacgt
     2761 cgccctccgc cagccacccg cgcatcgcct cgtaaccctc gcgctccgtg aagccgcagt
     2821 cgcgcacgtc gcccgcggcc aacgctatcc cgtgcttttc cagcgcgcgg cggtagcctt
     2881 cgaagcggcc cgcggcggtc gaggcgccgg tgccgcgcag gtgcccgatg cggcggtggc
     2941 cgaggccgac gaggtgttcg gtcgccagca tcccgacctg cacgtcgtcg gtcgtgaccg
     3001 cggggcagcg cacgccgtcg agccgccggt cgatgaagac gaccttcgcg ccgtccttga
     3061 cgagccggcg gtagaagccc gcgtccgatg gcggcagtga cgaggcgacg atgaggccgt
     3121 ccgtgcgcgg cagcagcgcg cccgtctcgt ccaactcttt ggccgggtct tcgtccgtgt
     3181 tgcagatcag gttgcggtag cccgaagggc gcgcgaagtc ctcgacgccc ctgcaaatct
     3241 ccgcgaagta ggagtgcatc aggtcgggca cgacgacgcc gatcaggtac gagcggttgg
     3301 tcgccagcgc gcgcgcgtgc tggttgggct ggtagttgag ccgccgcgcg acttcgagca
     3361 cgcgctcgcg cgtgtcggcg ttgatggagg ggtggttgtt gatggcgcgc gagacggtca
     3421 tcttcgagac gcccagctcg cgcgcgatgt ccgcaagagt taccggcatg gtcttctagt
     3481 cgtaaggccc ttcgtccgcc cgcatcttac atcccccgcc tgttttgcgc cacgaaagaa
     3541 ttcccggccc gcgccccgcg cccgcggcca ccccgttcgt aaattccttc cctccgcacc
     3601 gcttccgctt gatccctttc ccgatgtttt tgcgcgtata cgggtggagg cgaaagtcat
     3661 gggacaaaaa tatcaggtgc ggatcatcaa acggggccag cgggagagcg cggagagcgt
     3721 cgtggaggcg gcgacgccgg agcgggcgga gcgcgcggag ccttccgagc gcgagctgaa
     3781 gacggtcatg tccggctggg tgcgcgagca caggcagcgg acggaggagt accggcggtc
     3841 gttcgcgggc ctgctgcggc aggtcggctt caaccccctg cccgcgggca ggcgttcata
     3901 aaacgggcgg cgtcccggcg cgcaccgcag agacgcgccg gcgcgggatt acgaacacca
     3961 caccgcccgt catccttcat ccgccagtag ttacccgtct ttcaacattc gccacacgcc
     4021 gttacgcatc ccttcatcgg ctaccgccga agcccgtatg ccgggccgag cgcgacgccg
     4081 gaacgcttct cttccagcat ccttagaacg tagcgcgcga cctcttcagc cgccacctcc
     4141 gggcgcttgc ccctcgggtg gacgccgcag acggcgttcg tgaacgcgtg atccatgtcc
     4201 ggactccagc gcggacgccc cgccgcgtcg cgcccgtacg tcatgtaaac cgagagcgtg
     4261 tcgaggcccg tgcccggtcg ctcgccgatg aggtggacga ccacctccac gccgagcagc
     4321 gcgccgacgt ggtagcccgc gcgcacgcgc ccgttccgca caactacgtc tgcctcgccc
     4381 acgtgacagc ccgcctccga cagcgcggcg cgcagacgcg gcagcacccc gcgcaggttc
     4441 tcgttcagcg cgtccgcgtt caggccgtcc gagacgacga actgcacctg cggtcgcctc
     4501 gtcgaataaa gatcgcggat gcgcgccgcg tcttcgtcga ggaggcgctc gcccgacggc
     4561 gggtgcgcca ggaactcttc gcggtcgcgc gcgagcgtgc ggacgcgcaa ggcgcgcggc
     4621 gacgcttcgc gcaccaccgc ttcgtcgagc gtcgcgtaga gcgcccgccg cgcgtgacgg
     4681 tagatcgcgt cgagccgcgt ctcgacttcg cgcggcgccg cgaagtccgc gccgtggccg
     4741 tggccgaggt cgaagccgcg cccgcgcacg tcggcaagct tcttctcacc ttccgcgcgc
     4801 aactcttctt ccgtgcgcgt atcgccgccg gccttcatgt agaccgcgta gagcgcggcg
     4861 gggccgggcg cgtccggaag cacgtcgccg cccgcgtcca gcacgccgag cgcttcgagg
     4921 cggcgccgca tcggcgccgc gacgccgcga cgcgtcctcc ggcgcaaggc ggggtgctcg
     4981 cggaaggagg tcgtcaggta gccaagcatc gggtccgcgt tgcccgcgac ggccatcagg
     5041 taggcgggcg ccgcacgctc cacgatccgt tcggtcagcc gcgcgagctg cgccggctct
     5101 acgcccatgt ggaacgtcga gcacacgtcg agacccatcg tcagcccgtg cagcttcgcc
     5161 atcacggcgt cttcgaggca cgcgcgctca agctgctccc ccgtgcggaa gacctccggc
     5221 ccgatgaagc ccgccacgtc gttgacgatc atccacgcgc ctgtccgcct acgaatatga
     5281 cgggcgacgc cgtaggcgcg cgcttcgagc gtcagcatgt ccacgccctc ggcggcgttg
     5341 ttggtgacgg ctgacccctg accggtctcg aagtagaggc cgccgaagcc gcgcgccagt
     5401 tcgagcacgc cctcaacgtc cagcccgacc atgcctccga gcgccttcga cgttccggcg
     5461 aggctctgaa aacctacgtc aactttcacg cgcgcgcgcg cctccgactg cttcacgatg
     5521 tccgaaagga cgcagtagcg cgtcggcagt tccagacgct cgacgacgcg gcgcagcagt
     5581 tcttctaggc ggatgatggt ctccacgtcg tcgctcgcgg ggttgatgcc gagcacgaca
     5641 tcgccgcagc cgtaagagag accttcgagg acggagaaaa gaatctcctc ctcttcatcg
     5701 ccggggctgt tcggctggat gcgcgagccg aagtgcccgc gcgagccgat gcagacgccg
     5761 ccgttcgctt cgggcagcgg gttgaagaga gagcgcgcga ccgtcgagag ttcgtcgttc
     5821 gtcatcaact tggcgaccgc cgcgatgcat tcgctcgcca gcccttcgcg gtggcggccc
     5881 acccactccg ccccgcccgc gccgagcagc acgcccttca gcccgccgac ggtcaggccg
     5941 gagatgtcgg acgcgagccg cgcgtcgagc gcgcccgcga gcgcgtcgct cacgccgtcc
     6001 tcgacgagcg cggtgcgcgc gatgtcaccc agccgcaacg cggcgagcga gcggcgcgcc
     6061 tcgccgcgca cgccctcgtc gcgcgtcccg cccacggcca ggtcgccctc cttgaactcg
     6121 cccgcgagcg cgaaaatttg tcgtagctcg tcgccgttca ttcccgttcc gaagaaaaag
     6181 ccgtgactca aagactgtag gggcagggct tgtccctgcc cgcggtcacg gtacgttcga
     6241 cgttgaattc gatgaatcgt gacgggccgc ggacttgtcc ctgcccctac agtcttcacc
     6301 gacgtcgaat tctaaaacga acaccatttc taaaacgaac accattgaaa tcaggccgcg
     6361 ctcacggtcg cgcgtgcaac caaaaaggct tctcgcgcgt caaaatagca cgatcatcaa
     6421 ccccctgcat cgacagttga cggcgaccct tcgcgggaca ttcgaattgc catttcacag
     6481 gctgtttttc ccacaaaatt tcaagtaacg gacgacgcgc aggatacgtc ttactcttgg
     6541 agcgtcacgt catttctcac ttgacggatc gagtttttcg cccgcgtgca ggaacttatc
     6601 ggcgcgcgac acgtaacaaa taactggtca gagcgttcgg tcggcacatc ggtttttcat
     6661 catgacgaag atcaaaagca ttcttttcgc cctctcggcg gccctcctgt tcattgccgc
     6721 cgactcgcct gcgggcgcgc agacagcgaa gaagaagaag accgccgcgc agccccagca
     6781 ggccgcggcg gcggaggtcg cgagcgaccc ggagagcttc aagaaggact acatcaaggc
     6841 cgcggaggag tacgaggcga gcctgcgcga gttggccgct tcgtacgagg ggggcctcaa
     6901 gaagctgtcg gagaagcacg aggcgctgaa gggcctctac accgacggtc tgatcgcgcg
     6961 ccgcgagttc gaggagagcg agaaggcgct cgcggaggcg ggcgcgaagg tcgaggacgt
     7021 gcgccgcgaa atcgcctcgg tcgagaagac gctcgaagtc gcgcgccggc ccacgcccgc
     7081 cgtcgcgcct gacaccgccg cgctgccgcg cgtcgaggtc gcgtggacga ccggcaacgc
     7141 gaagatcgac ggcctcatcc gcctgaacgg ccagcgctac ggcgtcgatc cttatctcat
     7201 ctactgcatc atgcatcagg agtcgcgctt cggcgcgggc gcgaccagct acaagggcgc
     7261 gatgggcctg atgcagctca tgcccggcac cgccgcgcgc ttcggcgtga cgaaccccta
     7321 cgaccctgcg cagagcatca tgggcggcac gcgctatctc aaggagctgc tgcggctctt
     7381 cggcggtcgc gtcgatctcg cgctggcggg ctacaacgcg ggcgagggcg ccgtcatgaa
     7441 gtacggtcgc cgcgtcccgc cctaccgcga aacgcaggac tacgtccgca tcatcggcac
     7501 gcgctactcg aagggcgccg ccgtcccgac ggcgaaggtc accgagcgca aggcgggcgg
     7561 cgcgggcaag taacggacgc agggctttat cgcttgaatt cgaaagcggc gcgtgttcac
     7621 gacggaacac gcgccgcttt ttctttgcgg ccccttcaga agcgggcacc cgctcaacgc
     7681 gcggcggtcg tcgagggcgg gggcgcggtc ggcgcgggcg tcggcgtcgg cgtcggcgtg
     7741 ggcggcggcg cgggcatgcg ctctctgttg aagcgaggga gcatctcgtc gcgcgtggcg
     7801 aggtggtagg cggcggcggc gacgacgatg gcggcggcct tcacgtcgtc ctcgacgatg
     7861 cgctcgtagg tgtcgaggtt ggtgtgccag gtgtgcgtgc cgtactcgat ggggtcctgg
     7921 cccatgccga tgccgggcag gcccgcgttg ttgaaggagg tgtgatcgct cccgccgagg
     7981 ttgcggctgc gcgtggcgat ggcgccctcg acgccgaggt ctgcgaaggg cgcgagcgcc
     8041 tgccgcagga tgcccgcggc ctcgggcggg ccgaagacgg tcgcgccgcg gacgcggccc
     8101 gtgcccgagt cgatgttgaa atagccgttg aatttggcgt ggccgggctg cggtttttcg
     8161 aacgagccga aatacttctt gacgtaagcc tgcgagccca gaaggccctg ctcctcgccc
     8221 gaccagagcg cgacgcggat ggtgcggcgc ggcttggcgc cgacgacgcg gaggatgcgc
     8281 gcggcctcca tcatgatcgc gcagccgatg gcgttgtcgg tggcgcccgt cgccgcgtgc
     8341 cacgagtcga ggtggccgcc gagcatgacg atctcgtcct tgtcttcgtc gccgggtatc
     8401 tccgcgatga cgttgtagga ggtgcgcccc tccgggtagc tgcgattgac gatgtcgaat
     8461 tcgagatcga ccggcgtcga gtcggcgagg atgcgcgtga tgcgaccgta gtcttcgtta
     8521 cggcagacgg cggtcgggac ggccttcgac acgtcgaagg tgcggttgtt gaaggcgcgt
     8581 atctgcccgt gctcgcggcc cgcgtcgttg acacggacgc gggcgccgct ctcgacgagg
     8641 aacctatcga cggcctcggc caactgctga ttggtgagcg gctgcggctg gttcggcgcg
     8701 ggcgtcggcg aaggcgtcgg cgcggcggcg aagggcgaag gggtcgggtt gttggggtcg
     8761 aactggttgc gcgcttcctg ctcgtcgcgg cgcttgaagg gcgggttgaa ggagacgggc
     8821 agcggcgtgt gtttgccgac gaggacgatc ttgcccctga cctgttcctt gacggtggcg
     8881 aagtagaagt cgagctgctg gcgcgtgggc cgctcgggga cgacgatctg gacggcgcgc
     8941 gcgcggacgg ggccgctggt gccgggcgtc cacgccagcg cctcgcagac cagagagtcc
     9001 ttgaccggcg cgacgaggtg cacgctcagg cgctcgttct gccagccggg gtggccgaag
     9061 tcccacggtt cgagccgcgc gttccgcagg ccccacgtct ccatctgccg ggcggcccag
     9121 tcggctgccg ccttgtggtt gggcgagccg gtgaggcgcg gcccgtacac gtccgtcagg
     9181 aagtgcagcg tccgcatgat ctgcgagttc tccgtcccct cgcggcgaat cttccagaag
     9241 acctcggcgt tgatgcgctc ctgcctcgcg gcggcggcgt cctgcccgct cgccggcagc
     9301 gcgcaccaga cgcaaacgaa cgccagcact acggccatgc ctctcttcat cgcgcggctc
     9361 ccgtctcgtg tgaaaatgaa aacccggacg acgataagcg cctcccgtgt gaaagtctgt
     9421 tttcgttggc tgttccggtt gttataccac gcgcggcttg cgctttgaaa acggtcgagc
     9481 gttctgtagt ccgtggtcac aactgtaggg gcagggcaag t
//
LOCUS       DBNGOH010000685         5713 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1568355, whole genome shotgun sequence.
ACCESSION   DBNGOH010000685 DBNGOH010000000
VERSION     DBNGOH010000685.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5713)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5713)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5713
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1568355"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="AC159P_13270"
     CDS             complement(<1..189)
                     /locus_tag="AC159P_13270"
                     /inference="COORDINATES: protein motif:HMM:NF025078.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="eCIS core domain-containing protein"
                     /protein_id="HIR3696463.1"
                     /translation="MSIVPASLGQPLEAGVARKLGSLLGHDFSRVRVHRDAAADHAAR
                     SVDALAYTVGSDLVFAAGE"
     gene            complement(522..1913)
                     /locus_tag="AC159P_13275"
     CDS             complement(522..1913)
                     /locus_tag="AC159P_13275"
                     /inference="COORDINATES: protein motif:HMM:NF025078.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="eCIS core domain-containing protein"
                     /protein_id="HIR3696464.1"
                     /translation="MWAAIARDINEIAAAKKQAAKPPPEIVSHVPAHPSHTGLTLQRK
                     AACACGGSCPRCAEESEHAVLQTKLRIGTPGDEYEREADRVADAVTSLPELHTATVET
                     LSAHASSATHSDGSVARRYTPCGVGDEVARVDLVPGGGEPLAPSAKALFEPRFGHDFG
                     NVRVHTDPRAADSARRMNALAYTFGRDIVFGAGMYEPGTPWGRKLLAHELAHVVQQSG
                     GGAATGAVNRPAPLHVQRQATDPDLSGPCSDDRSENIIEPAFTEARRWRRLTAAWLEA
                     HLDHIRARGALARDGYVRIGRRVFDELMLLQRHFRISDVLRVSLPYTADDLVSVPDLQ
                     RYGNASYWVRRRFDEVELTLSYLCQTNCPRGRTGSDLLGSAVAGMKEVIFYTNCFDGQ
                     HETTRAGTALHEAFHAGFSEFNHDTYYFEGGYPGDDPLTNAESFATFAAFVATGSNYR
                     ITNVPGITIHGNQ"
     gene            complement(1963..2898)
                     /locus_tag="AC159P_13280"
     CDS             complement(1963..2898)
                     /locus_tag="AC159P_13280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HIR3696465.1"
                     /translation="MSSKRTEFTLEEITRELEQKVGGADKLRADGLEQLGAVRRAREA
                     GLRREQARLTKKHGAEHPSVVAITNRLTANTGMVRDLGLEASRARVQPPRVDARGWAV
                     HGFVRDKELKPVGGLTVALYDAQSRWVEQAGFACTKADGYFLIRLNDAGALPGPLFLR
                     VLSGQAVHLFADPSPLTPTTGAVVSREIFLSEDGRACVPPVTGTSNPVAAAGAWVVRG
                     RITDAKGAGRGGLTVSIFDKDSVFTDRLGQTKTDENGDYSFTFDTENFRDLIERKPDL
                     FLKVLDPNGRTLFAAEQAAIRFQAGRVEVIDLTLS"
     gene            complement(3005..>5713)
                     /locus_tag="AC159P_13285"
     CDS             complement(3005..>5713)
                     /locus_tag="AC159P_13285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HIR3696466.1"
                     /translation="AIVNSGLQEFYLEALAFDPAPTQPTGLGHWRLVYGARVTLNSAT
                     GVFSLPDPLVANSGHLFGAGIPPSSASFFFRLWNGVEPVADFDGPAPLPLQDGILLQF
                     DPAAGANYVAGDYWTFSVRAGEIDNEPPLVDDQPPEGIHYHRVPLGILQWQAPVTPPL
                     IEDCRHVFQPLTKLATCCTYRVGDGTRSHGDFTSIQAAVDALPAAGGEVCVLPGVYVE
                     NVRIERRRNVTIKGCGPRSRVVSPPGANNLPAAPVFFVTESQDIEILSLAVEAHDTGI
                     GILLDGRRVIPAPDQEQRVRAPLFNVTLEELLVRAATRCAIEAHDANFTTVRRCHVEM
                     KDVPTSFPAVFFIGDDGLIEDNLILVITNIINRPVVAFQPQAPVQSFLLGRAEAALGG
                     LQLGGGCERVRVLDNVIARGIGIGIKLGSLVVVNSDGEPLPEQPDPDPDPCFPCRPGD
                     NTVPGGDPDGTSRTVSEGDLHEIEITGNRIYRMGLDGIGVAGFFPLGQIDEFISVHGL
                     RIISNEIRLCLYRPLLPIPDAMLDAMGYGGISLADVSDLVIRDNFIHDNSPDFSEPVC
                     GIFVLHGEALEISRNHIFDNGTRPVILRQTNAALKRGPRGGIHIRFAIAPVVPINFVL
                     GRLNFTGTAQSGIPALRVHDNVVSVPVAGRALTVTALGAVSVVGNQFSTRGVGLIGVD
                     TFDGSTVWIMNLGISNEFFLQLLIFLLILIGNNQPPGGGRPGLDDATIGRSLIGGNVL
                     FAANQCTLDLMQAGASFTDTSIFIGTLDDLGFHDNQCECNLLDDFVLAQAVLFAISVR
                     ASDNRFKENMSLDGERFTSLFSAITLGIFNTTTDNQSTRCLFIIGFPNLTVDHSNVSL
                     LMLSNPNACCNFLVNRENCVSRGAATVNPVNPPPNQAVTTVTLARG"
BASE COUNT          881 a         1783 c         2103 g          946 t
ORIGIN      
        1 ctcacccgcg gcgaagacca ggtcggagcc gacggtgtag gcgagcgcgt ccaccgaacg
       61 cgcggcgtgg tcggcggcgg cgtctcgatg cacgcggacg cggctgaaat catgaccgag
      121 gagcgaaccc agcttccgcg cgacccccgc ttcgagcggt tgcccgagcg acgcggggac
      181 gatgctcaca gccgaaggcg gagaaggtgc gcggtcagcg cggtcgccgc ctgcgcgctc
      241 gtgcgctccg tccggactgc ggaggcgcga cgcaccgtgc gggccgggcg gccccccctt
      301 gcagcgcggg caaccgccgc cgcaggcgca cgccgccttg cgcagtactc gtgggagttc
      361 cgcgtgcgag tcgtgtgtgc ccggcggaac ggcgcgggcg cgcgttcggt cgcaggcacg
      421 gagcggagct tcctgcacga ttgagcgaag ggccggtgct ttcatgaaat ctcttcgaaa
      481 ccgcttcccg ccccgcccga cggtgacggc ccggcgccgg cctactggtt gccgtgaatg
      541 gtgatgcccg gaacatttgt gatgcggtag ttgctgccgg tcgccacaaa ggcggcgaag
      601 gtggcgaacg attcggcgtt cgtcagcggg tcgtcgccgg ggtaacctcc ctcgaagtag
      661 tacgtgtcgt ggttgaactc ggaaaagccg gcatgaaacg cttcgtgcaa cgccgtaccc
      721 gcccgcgtgg tttcatgctg cccgtcgaag cagttcgtgt agaagatgac ttctttcatt
      781 cccgccacgg cgctgccgag caagtcgctg cccgtcctgc cgcgcgggca gtttgtctga
      841 cacaggtaag agagggtgag ttcgacttcg tcgaagcgac ggcgcaccca gtagctggcg
      901 ttaccgtagc gttggaggtc aggcacgctg accaggtcgt cggctgtata gggtaatgag
      961 actcgcagca cgtcgctgat ccgaaaatgc ctctgaagca acatcagctc gtcgaagacc
     1021 cgccgcccga tcctgacgta tccgtctcgg gccagcgccc ctctggcgcg aatgtggtcg
     1081 aggtgggcct caagccacgc cgccgtcagg cggcgccacc tgcgcgcctc ggtgaaggcc
     1141 ggctcgataa tgttctcgct gcggtcatcg ctgcacggcc cgctcaggtc tgggtctgtg
     1201 gcctgccgct ggacgtgcag cggcgccgga cgattcactg cgccggtcgc cgccccgccc
     1261 ccgctttgct ggacgacatg cgccagttca tgcgccagca actttcttcc ccacggcgtc
     1321 cccggctcgt acattccggc tccgaaaacg atgtcgcggc caaacgtgta ggcgagcgcg
     1381 ttcatccggc gggccgaatc ggcggcgcga gggtcggtgt gaacgcggac gttgccgaag
     1441 tcgtgaccga agcgcggctc gaagagcgcc tttgcggacg gagccagcgg ctcgcccccg
     1501 ccgggcacga gatcgacccg cgccacttca tcgccaacac cgcacggggt ataccgccgc
     1561 gcgacgctcc cgtccgagtg cgtcgcggat gaagcgtgtg cgcttagggt ttcgacggtc
     1621 gcggtgtgaa gctcgggcag gctcgtcacc gcatcggcca cacggtcggc ctctcgctca
     1681 tactcgtcgc cgggagtgcc gattcgtagc ttggtctgaa gaacggcgtg ctccgactct
     1741 tccgcgcagc gcgggcagct tccgccgcag gcgcacgccg ccttgcgctg caacgtcagg
     1801 cccgtgtgcg aggggtgggc gggtacgtgc gagacgatct cgggcggagg cttcgcggcc
     1861 tgctttttcg cggcggcgat ctcgttgata tctctggcga tggctgccca catgaattcg
     1921 gcctccgccg ctgcccatca gcttcggtgt cccgcgcccg cgtcaagaga gtgtgaggtc
     1981 gatgacctcg acgcgcccgg cctggaacct gatcgccgcc tgttccgcgg cgaagagcgt
     2041 gcgcccgttg gggtcgagca ctttgaggaa gaggtctggc ttgcgctcga tgaggtcgcg
     2101 gaagttttcc gtgtcgaagg tgaagctgta gtcgccgttc tcgtccgtct tggtctggcc
     2161 gagacggtcg gtgaagactg aatccttgtc gaagatgctg acggtcagcc cgccgcggcc
     2221 cgcccctttc gcgtcggtga tgcgtccgcg gacgacccac gcgcccgccg cggcgaccgg
     2281 attgctcgtg ccggtgacgg gcggcacgca cgcacgcccg tcttcggaga gaaaaatctc
     2341 gcgcgagacg acggcgcccg tggtcggcgt gagcggcgac gggtcggcga agaggtgcac
     2401 ggcctgcccc gacagcacgc ggaggaagag cggccccggc agcgcgccag cgtcattcag
     2461 ccggatgagg aagtagccgt ccgccttcgt gcaggcgaag cccgcctgct cgacccatct
     2521 cgactgcgcg tcgtagagcg cgaccgtgag gccgcccacc ggcttcaact ccttgtcgcg
     2581 gacgaagccg tggacggccc atccgcgcgc gtccacacgc ggcggctgga cgcgcgcgcg
     2641 cgacgcctca aggccgaggt cgcgcaccat gccggtgttc gccgtcaggc ggttcgtgat
     2701 ggcgacgacg ctcgggtgct ccgcgccgtg cttcttcgtc agcctcgcct gctcgcgcct
     2761 gaggcccgcc tcgcgcgcgc ggcgcacggc gccgagttgt tccagcccgt cggcgcgcag
     2821 cttgtcggcg ccgccgacct tctgctccaa ctcgcgcgtg atctcttcga gcgtgaactc
     2881 tgtccgttta ctactcatcg gcggacgcct cccgcgtgga ttatgaagtt cgtgtgcgca
     2941 gcctcgcgcc gaagagtcta agcccggcgg gcggcgcacg gtcaagcgcg gtcagtcggt
     3001 ctcgctaacc gcgtgcgagc gtgacggtcg tgaccgcttg gttcggcggc gggttcacgg
     3061 ggttgaccgt ggccgcgccg cgcgaaacgc aattctcccg gttgacgagg aagttgcaac
     3121 aagcgttcgg gttgctgagc atcaacaggc tgacgttcga atgatccacg gtcaggttcg
     3181 ggaagccgat aatgaagaga cagcgcgtgg actggttgtc ggtggtcgtg ttgaaaatgc
     3241 cgagggtgat ggccgagaag agagaggtga atcgctcgcc gtcgagcgac atgttctcct
     3301 tgaagcggtt gtcgctcgcg cgcaccgaga tggcgaagag cacggcctgc gccagcacga
     3361 agtcgtcgag cagattgcac tcgcactggt tgtcgtggaa gccgaggtcg tcgagggtgc
     3421 cgatgaagat ggacgtgtcg gtgaagctgg ctccggcctg catgaggtcg agcgtgcact
     3481 ggttggcggc gaagaggacg ttgccgccga tgagcgacct gccgatggtc gcgtcgtcga
     3541 ggccgggccg cccgccgccc ggcggctggt tgttcccgat gaggatcagc aggaagatga
     3601 gcaactgcaa gaagaactcg ttcgagatgc cgaggttcat gatccagacg gtcgagccgt
     3661 cgaaggtgtc cactccgatc aggcccacgc cgcgcgtgct gaactggttg ccgacgaccg
     3721 agaccgcgcc gagcgccgtc acggtgagcg cgcgaccggc cacggggacg ctcaccacgt
     3781 tatcgtgcac gcgcagcgcg gggatgccgc tctgcgccgt gcccgtgaag ttgaggcgac
     3841 cgaggacgaa gttgatgggg acgacgggcg cgatggcgaa tctgatgtgg atgccgccgc
     3901 gcggaccgcg cttgagggcc gcgttcgtct gccgcaagat gacgggacgc gtgccgttgt
     3961 cgaagatgtg gttgcgcgag atttccaacg cctcgccgtg gagcacgaag atgccgcaga
     4021 cgggttcgct gaagtcgggg ctgttgtcgt ggatgaagtt gtcgcggatg acgaggtcgc
     4081 tcacgtccgc gagcgagatg ccgccgtagc ccatcgcgtc gagcatcgcg tcggggatgg
     4141 gcagcagcgg gcggtagagg cagaggcgaa tctcgttcga gatgatgcgc aggccgtgca
     4201 cgctgatgaa ctcgtcgatc tggccgagcg ggaagaagcc cgcgacgccg atgccgtcga
     4261 ggcccatccg gtagatgcgg ttgccggtga tctctatctc gtggaggtcg ccctccgaga
     4321 cggtacgcga cgtgccgtcg gggtcgccgc cggggacggt gttgtcgccg gggcggcatg
     4381 ggaagcatgg gtcggggtcg gggtcgggct gctcgggcag tggctcgccg tcgctgttga
     4441 cgacgacgag gctgccgagc ttgatgccga tgccgatgcc gcgcgcgatg acgttgtcga
     4501 gcacgcggac gcgctcgcag ccgccgccga gttgcagccc gccgagcgcc gcctcggccc
     4561 tgcccaagag aaacgactgc accggcgcct gcggctggaa ggcgacgacg ggccggttga
     4621 tgatgttcgt gatgacgagg atcaggttgt cttcgatgag gccgtcgtcg ccgatgaaga
     4681 agacggcggg gaagctggtc ggcacgtcct tcatctcgac gtggcagcgg cggacggtcg
     4741 tgaaattggc gtcgtgcgcc tcgatggcgc agcgggtcgc ggcgcggacg agcaattctt
     4801 cgagcgtgac gttgaagagc ggcgcgcgca ccctctgctc ctggtcgggg gcggggatga
     4861 cgcgtctgcc gtcgagcagg atgccgatac ctgtgtcgtg ggcctcgacg gcgagcgaga
     4921 ggatttcgat gtcctgcgat tcggtgacga agaagacggg cgccgccggc aagttgttcg
     4981 ctccgggcgg cgagacgacg cggctgcgcg ggccgcagcc cttgatcgtg acgttgcgcc
     5041 gccgctcgat gcgcacgttc tccacgtaga cgccgggcag gacgcacacc tcgccgcccg
     5101 cggcgggcaa ggcatcgacg gcggcctgaa tgctcgtgaa gtcgccgtgg ctgcgcgtgc
     5161 cgtcgccgac gcggtaggtg cagcaggtgg cgagtttcgt gagcggctgg aagacgtggc
     5221 ggcagtcttc gatcagcggc ggcgtgaccg gggcttgcca ctggaggatg ccgagcggga
     5281 cgcggtggta gtggatgccc tcgggcggct ggtcgtccac gagcggcggc tcgttgtcga
     5341 tctcgcccgc gcggacggag aacgtccagt agtcgcccgc gacgtagttg gcgcccgccg
     5401 ccgggtcgaa ctggaggagg atgccgtcct gcaaaggcag cggcgcgggg ccgtcgaagt
     5461 ccgcgacggg ttcgacgccg ttccagaggc ggaagaagaa ggaggcggac gaaggcggga
     5521 tgcccgcgcc gaagagatgg ccggagttgg cgactaaggg gtcgggcagc gagaagacgc
     5581 cggtggcgct gttgagcgtg acgcgcgcgc cgtagacgag ccgccagtgg ccgaggcccg
     5641 tgggctgcgt gggcgcgggg tcgaaggcga gggcttcgag gtagaactct tgcaggccgg
     5701 agttgacgat ggc
//
LOCUS       DBNGOH010000686         3163 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_5050994, whole genome shotgun sequence.
ACCESSION   DBNGOH010000686 DBNGOH010000000
VERSION     DBNGOH010000686.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3163)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3163)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3163
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_5050994"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            24..170
                     /locus_tag="AC159P_13290"
     CDS             24..170
                     /locus_tag="AC159P_13290"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR3696467.1"
                     /translation="MDVLKRMSPQNRLVTPEEVAALALLLASEDGRGITGQGINVDGG
                     SVMF"
     gene            288..686
                     /locus_tag="AC159P_13295"
     CDS             288..686
                     /locus_tag="AC159P_13295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011242177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HIR3696468.1"
                     /translation="MSFEIINPASLGRPRGYANGLLTAPGARLLFVAGQIAWDEEQRI
                     VSADFVEQFERALRNVLAVVGEAGGEAGGVARLVVYVTDKEEYRARTGEIGERWRALM
                     GRHYPAMALVEVKGLLEDGAKVEIEAVAVL"
     gene            809..1600
                     /locus_tag="AC159P_13300"
     CDS             809..1600
                     /locus_tag="AC159P_13300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009026169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase family protein"
                     /protein_id="HIR3696469.1"
                     /translation="MIETKSFLYEERNGVAWITLNRPERLNALTFEVYRELTDTFAAL
                     RDEAEVRAVVITGAGRAFSSGGDVRDIIGGLQGRDMEGLLEFTRMTCELVRNIRALRK
                     PVIASLNGTTAGAGACIALASDLRIASTEARIAFLFVRVGLSGADMGAAHLLPRLVGL
                     AKATELLYTGDFIDAAEAHRIGLYNHVFAPHELEAETRALAHRLARGPAFALAVTKEM
                     LDREMDTSFDTALELEAQAQAVCMQHPDYNEAYEAFMAKREPRFK"
     gene            1704..2870
                     /locus_tag="AC159P_13305"
     CDS             1704..2870
                     /locus_tag="AC159P_13305"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018913343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR3696470.1"
                     /translation="MDAFIEQTPFFTDDHARLAAGVSAFVAREIEPRMVEEEEQGEDD
                     FFRETLALLAQADLLRFSVARGDGTPLDARALCVVREALAYSSAVADLAFVMQGLGTY
                     ALSLAAPEHVRDFWLGRARAGKAVAALALTEPNAGSDVSAIETSAVRDGESYVINGEK
                     RLISNAGVADFYTVVARTGTRRDGRPDLSAFIVGARMPGFSVRERTRLISPHPIGVVE
                     FKDLRVPAEDCVGAEGEGFRLAMQTMDMFRASVGAAACGMARRAFDEALRHAKTRRQF
                     GAALAEQQLIQAKLADMQTELDAARLLVYRAAYLKDTGGPGSQLTRAVSQAKLYATEA
                     AWRIIDEAVQIHGGEGVVHGSVVERLYRDVRALRIYEGTSEIQKLIIARELLRD"
     gene            2897..3160
                     /locus_tag="AC159P_13310"
     CDS             2897..3160
                     /locus_tag="AC159P_13310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696471.1"
                     /translation="MSQAKAYICVVLMQHENKHESRLLTDLVAAPDEEQALVKASLLA
                     HPGGHDNWTVLSKEVHEVDRDTLRRAAAEVLGWTDAAGAGTTA"
BASE COUNT          548 a         1057 c         1085 g          473 t
ORIGIN      
        1 cgggccgcac gcgcgaagag gccatcgacg tgctcaagcg gatgagtccg cagaaccgcc
       61 tcgtcacgcc agaagaggtc gccgcgctcg cgctgctgct cgcctccgaa gacgggcgcg
      121 gcatcaccgg ccagggcatc aacgtggacg gcgggagcgt catgttttga ttttggattg
      181 cggaatgcgg aatgcggatt ggtcaggcgc cgaggtttga gcgtccgaaa tcgagcttca
      241 agcatcggca acacgccaat ccgcattccg cattccgcct tcgtctcatg tctttcgaaa
      301 tcatcaaccc cgcgtcgctc gggaggccgc gtgggtacgc gaacgggctg ctgacggcgc
      361 cgggcgcgcg gctgcttttc gtcgccgggc agatcgcgtg ggacgaagag cagcgcatcg
      421 tgagcgcgga cttcgtcgag cagttcgaac gcgcgctgcg gaacgtgctg gcggtcgtcg
      481 gcgaggcggg cggcgaggcg ggcggcgtcg cgcggctcgt cgtctacgtc acggacaaag
      541 aagagtatcg cgcgcgcacc ggcgagatcg gcgagcgctg gcgcgcgctg atgggccgcc
      601 actaccccgc gatggcgctc gtcgaagtga agggcctgct cgaagacggg gcgaaggtcg
      661 agatcgaggc cgtcgccgtt ctttagtgaa tggtaaatgg tgaatagtaa atagcggtcg
      721 agcggcattc ggccatgagg agttaatcgg cggcgcggca tgagggccgt tttccattta
      781 ccattgacca ttcaccattt accaaccgat gattgagacc aaatcattcc tctacgaaga
      841 gcggaacggc gtcgcgtgga tcacgctgaa ccgcccggag cgcctgaacg cgctgacgtt
      901 cgaggtctac cgcgagctga cggacacgtt cgccgcgctg cgtgacgagg cggaggtgcg
      961 cgcggtcgtc atcacgggcg cggggcgcgc gttctcgtcg ggcggcgacg tacgcgacat
     1021 catcggcggg ttgcaggggc gcgacatgga ggggctgctg gagttcacgc gcatgacgtg
     1081 cgagctggtg cggaacatac gcgcgctaag gaagcccgtc atcgccagcc tcaacggcac
     1141 gaccgcgggc gcgggcgcct gcatcgcgct cgcctccgat ttgaggatcg cgagcaccga
     1201 ggcgcgcatc gccttcctct tcgtcagggt cggtctctcc ggcgccgaca tgggcgccgc
     1261 gcacctgctg ccgcgcctcg tcggcctcgc caaggcgacc gaactgctct acacaggcga
     1321 tttcatcgac gccgccgaag cgcaccgcat cggcctctac aaccacgtct tcgcgcccca
     1381 tgaactcgaa gcggagacgc gcgccctcgc gcacaggctc gcgcgcggcc ccgccttcgc
     1441 cctcgccgtc acgaaggaga tgctcgaccg cgagatggac acgagcttcg acaccgcgct
     1501 cgaactcgaa gcgcaggcgc aggccgtctg catgcagcac ccggactaca acgaagccta
     1561 cgaggctttc atggcgaagc gcgaaccgcg gttcaagtga gcagtgagcg gtaagcagtg
     1621 agcagtttaa aactacatgc tcaccgtccg tcgcaaccaa caaaacgact gcttaccgct
     1681 cactgctcac ggcttacttt tttatggacg cattcatcga acagactccg ttcttcaccg
     1741 acgaccacgc gcggctggcg gcgggcgtga gcgccttcgt cgcgcgcgag atcgagccgc
     1801 gcatggtgga ggaagaggag cagggcgagg acgacttctt ccgcgagacg ctcgcgctgc
     1861 tggcgcaggc cgacctgctg cgcttctcgg tggcgcgcgg cgacgggacg cccctggacg
     1921 cgcgcgcgct ctgcgtcgtc cgcgaggcgc tggcctactc gtcggcggtg gccgacctcg
     1981 ccttcgtcat gcagggcctc ggcacctacg ctttgagcct cgccgcgccc gaacacgtgc
     2041 gcgacttctg gctcgggcgc gcgcgcgcgg gcaaggcggt cgcggcgctg gcgctgacgg
     2101 agccgaacgc cggatcggac gtgtcggcca tcgagacgag cgccgtgcgc gacggcgaga
     2161 gctatgtcat caacggtgag aagcgcctca tctcgaacgc gggcgtggcg gacttctaca
     2221 cggtcgtcgc gcgcacgggc acgcggcgcg acgggcggcc agacctctcg gccttcatcg
     2281 tcggcgcgcg gatgcccggc ttcagcgtgc gcgagcgcac acgcctcatc tcgccgcacc
     2341 ccatcggcgt ggtcgagttc aaagacctgc gcgtgcctgc ggaagactgc gtcggggccg
     2401 agggcgaggg cttccgcctc gcgatgcaga cgatggacat gttccgcgcg agcgtcgggg
     2461 ccgccgcctg cggcatggcg cggcgcgcgt tcgacgaagc gctccgccac gccaaaacgc
     2521 gccgacagtt cggcgccgcg ctcgccgagc agcagctcat ccaggccaag ctcgccgaca
     2581 tgcagaccga actcgatgcc gcgcgcctgc tggtctaccg cgccgcttac ttgaaagaca
     2641 cgggcggccc cggctcgcag ctcacgcgcg ccgtcagtca ggccaagctc tacgcgaccg
     2701 aggccgcgtg gcgcatcatc gacgaggccg tgcagattca cggcggcgag ggcgtcgtcc
     2761 acggcagcgt cgtcgagcgc ctctaccgcg acgtgcgcgc gctgcgcatc tacgaaggca
     2821 cgtccgagat tcagaagctc atcatcgcgc gcgagctgct gcgggactga ccgtcaaaca
     2881 ggggggaggc atccgaatga gccaagcgaa agcgtacatc tgcgtcgtgc tcatgcagca
     2941 cgagaacaaa cacgagagcc gcctgctcac agacctcgtc gccgcgcccg acgaagagca
     3001 ggccctcgtc aaggcgtcgc tgctcgcgca ccccggcggc cacgacaact ggacggtcct
     3061 gagcaaagag gtgcacgagg tggaccgcga caccctccgg cgcgcggcgg cggaagtgct
     3121 cggctggacc gatgcggcgg gcgcgggcac gacggcctga aca
//
LOCUS       DBNGOH010000687         2276 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_651320, whole genome shotgun sequence.
ACCESSION   DBNGOH010000687 DBNGOH010000000
VERSION     DBNGOH010000687.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2276)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2276)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2276
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_651320"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(62..706)
                     /gene="ribA"
                     /locus_tag="AC159P_13315"
     CDS             complement(62..706)
                     /gene="ribA"
                     /locus_tag="AC159P_13315"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014101377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HIR3696472.1"
                     /translation="MKEEEILDCPWAKQALTVERVSSARLPTEAGDFLIAGYRSTTGS
                     NEEFVVLYKGEIEPEVPTLVRIHSQCLTGDVFGSTKCDCGRQLHRAMELIEREGRGAI
                     VYQLQEGRGIGIINKIRAYALQDEGADTVEANERLGLAVDLREYRQCAEILFDLGLCK
                     VRVMSNNPLKLRAMEEAGLRVVDRVPLEVEPGEAAVHYLRTKKEKLGHLLETIK"
     gene            903..1886
                     /locus_tag="AC159P_13320"
     CDS             903..1886
                     /locus_tag="AC159P_13320"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HIR3696473.1"
                     /translation="MSSDPEDITIVNEAPRAEAVRKHPALVFLRGDLMAAPIPLEREV
                     VTLGRALEADVRVNDARASRMHARITVEREPHTGERRYRLADLGSTNGTLLNGRAVAE
                     AFLADGDKLTIGEHLLRFDLLDDIDREFQTHIHRLLAHDELTGLLTSKSFFSELRREA
                     ARAESESRPFCVLMMDLDHFKSVNDRHGHLVGSQTLEDVGAIITRSMRAGDVAARFGG
                     EEFAAFLLNADCAQGLVAAERVRSAVEEHLFNAARRGSTADTGEVLRVTISIGVAAYP
                     DDARDPIQLIELADTALYRAKNAGRNRVCAYCEPPSDALTGEAAPLRPRED"
BASE COUNT          347 a          830 c          715 g          384 t
ORIGIN      
        1 gctggcgcct gtccgtctcg tatccttttt aaatgtggtg ccgggcggcg gggcgcgcgg
       61 gctacttgat ggtttcgagc aggtggccga gcttctcctt cttcgtgcgg aggtagtgga
      121 cggcggcctc gcccggctcg acttcgagcg ggacgcgatc cacgacgcgc aggcccgcct
      181 cctccatcgc gcgcagcttg agcgggttgt tcgacatcac gcgcaccttg cacagcccga
      241 ggtcgaacag aatctcggcg cactgcctgt actcgcgcag gtcaacggcg aggcccagcc
      301 gctcgttggc ctcgaccgtg tcggcgccct cgtcctgcaa ggcgtaggcg cgaatcttgt
      361 tgatgatgcc gatgccgcgc ccctcctgta attggtagac gatggcgccg cgcccctcgc
      421 gctcgatcag ctccatcgcg cggtggagct gccgcccgca gtcgcacttg gtcgagccga
      481 acacgtcgcc cgttagacac tgcgagtgga tgcgcaccag cgtcggcacc tccggctcga
      541 tctctccttt atataagacg acgaactcct cgttgctccc ggtggtcgag cggtagcccg
      601 cgatcaggaa atcgcccgcc tcggtcggca gccgcgccga cgacacgcgc tcgaccgtca
      661 gcgcctgctt cgcccacggg cagtctaaaa tctcttcttc tttcaacgct cgaactcctt
      721 acgatggccg ccttgaatca agtgacccga ttataagagc attcggcggc aagacccaag
      781 caagcaggcg acgggcggtc cgcctgcggc gaagccgcta ttgccgcgcc gcccgccagc
      841 gcgctaagat gcgggccttg agagggaagc cgtcttcgcc cgccggacgg gcttttatcc
      901 cgatgagcag cgaccccgaa gacatcacca tcgtcaacga ggcgccgcgc gccgaggccg
      961 tgcgaaaaca ccccgcgctc gtcttcctgc gcggcgacct gatggccgcg ccgatcccgc
     1021 tcgaacgcga ggtggtgacg ctggggcgcg cgctcgaagc cgacgtgcgc gtcaacgacg
     1081 cccgcgcctc gcgcatgcac gcgcggatca cggtcgagcg cgagccgcac accggcgagc
     1141 gccgctaccg actcgcggac ctcggctcga ccaacggcac gctcctcaac gggcgcgcgg
     1201 tcgccgaagc cttcctcgcc gacggcgaca agctcaccat cggcgaacac ctcctgcgct
     1261 tcgaccttct ggacgacatc gaccgcgagt tccagaccca catccaccgc ctgctcgcgc
     1321 acgacgagct gaccggcctc ttgaccagca agtccttctt ctccgagctg aggcgcgagg
     1381 ccgcgcgcgc cgagagcgag tcgcgcccct tctgcgtcct catgatggat ttggatcact
     1441 tcaagtcggt caacgaccgc cacggccacc tcgtcggcag ccagactttg gaagacgtgg
     1501 gcgcgatcat cacgcgctcg atgcgcgcgg gcgacgtggc ggcgcgcttc ggcggcgagg
     1561 agttcgcggc cttcctgctc aacgccgact gcgcgcaagg gttggtcgcc gccgagcgcg
     1621 ttcgctccgc cgtcgaagag cacctgttca acgccgcgcg ccgcggctcg accgccgaca
     1681 cgggcgaggt gctgcgcgtc accatcagca tcggcgtcgc cgcctacccg gacgacgcgc
     1741 gcgaccccat ccagctcatc gaactcgccg acacggccct ctaccgcgcc aagaacgcgg
     1801 gccgcaaccg cgtctgcgcc tactgcgaac cgccctcgga cgcgctcacg ggcgaagcgg
     1861 ccccgctccg cccacgcgaa gactgattga tgggaagcac ggtgcctctc gtcatcccgc
     1921 ttcgcctttt tgcaactcgg catggggcgg cgcgtgtaat ggcgcgtgtg ggggacgggc
     1981 gcgcggcggc gcgaggcgcg ggcagcgggc ggcggcttga actttcgcct tttgcctttt
     2041 tacttttgcc ttcccgccgc gttatccttt tcgggtcggg cgaagcctcc cctccggctt
     2101 tcgtgacatc tatacaacag tgatcgccaa actcaatctc gccagccgac ctttccgcaa
     2161 ccgcacgctg ccgtggacgg cggcggtcgt cgtgtcagcc gcctcgctgc tcgcgctcgt
     2221 cgtccgggta ggcggcgacg ccgatgctga tggtgacgcg cagcacctcg cccgtg
//
LOCUS       DBNGOH010000688         1805 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3584547, whole genome shotgun sequence.
ACCESSION   DBNGOH010000688 DBNGOH010000000
VERSION     DBNGOH010000688.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1805)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1805)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1805
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3584547"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..828
                     /locus_tag="AC159P_13325"
     CDS             <1..828
                     /locus_tag="AC159P_13325"
                     /inference="COORDINATES: protein motif:HMM:NF013100.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HIR3696474.1"
                     /translation="LPSALGATEVPLDQMVSAYSAFANKGVRHEKHLIRRVLDRDGNT
                     LEEWEKTTFKVTSEYVALTMVDMMRGVTSGGGTAPAASAVGVPVAGKTGTVNDHTDVW
                     FIGYTPTYVTGVWMGYPERKQSLGSGFTGGRGALPIWIDYMKDFLKDKPKEQFEKPPK
                     MPEDIRELFLQRQREMAEERSLFLSARGARSDAENRALPAIAIEPKLEQLTLPPAPGE
                     AMTGTTTAAPADTTPARPVVAPRTGGGDDDAPPPPTAKPPRDPDPPKKKGKKGSDEP"
     gene            complement(940..1668)
                     /locus_tag="AC159P_13330"
     CDS             complement(940..1668)
                     /locus_tag="AC159P_13330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HIR3696475.1"
                     /translation="MERRITSWYSPNLGMEMPLVAYGHSGYPLLMFPTAAADFLEYER
                     FYLVDSIKHHIEAGRLRAYSINSVNRYSLLNDKAPPHIKGELLTRYDRYITDEVLPLI
                     RNDAGDQRPMTTGASLGAYLAANTYFKHPDILRGTIAMSGSYDVRSWFDGYHDDNVYF
                     NNPVQYLQNLDDGYFLPRLRQADAIVILSGQGAYEAPERSRQLSGILSWKGVPHHLDI
                     WGHDVNHDWPWWRKMLPYWLDRLL"
BASE COUNT          321 a          603 c          600 g          281 t
ORIGIN      
        1 ctcccgtcgg ccctcggcgc caccgaggtg ccgctcgacc agatggtctc ggcctactcg
       61 gccttcgcca acaagggcgt gcgccacgag aagcacctca tccgccgggt gctggaccgc
      121 gacggcaaca ccctcgaaga gtgggagaag acgaccttca aggtgacgag cgagtacgtc
      181 gcgctgacga tggtggacat gatgcgcggc gtcacttcgg gcggcggcac ggcccctgcg
      241 gcctccgcgg tcggcgtgcc cgtggcgggc aagaccggca ccgtcaacga ccacacggac
      301 gtgtggttca tcggctacac gccgacctac gtgacgggcg tctggatggg ctaccccgag
      361 cgcaagcagt ccttggggag cggcttcacg ggcgggcgcg gcgcgctccc gatctggatc
      421 gactacatga aggacttcct gaaggacaag ccgaaggagc agttcgagaa gccgcccaag
      481 atgcccgaag acatccgcga gctgttcctc cagcgccagc gcgagatggc cgaagagcgc
      541 tcgctcttcc tctcggcgcg cggcgccaga tcggacgccg agaaccgcgc gctgcccgct
      601 atcgccatcg agccgaagct cgaacagctc acgctcccgc ccgcgcccgg cgaggcgatg
      661 accggcacga ccaccgccgc ccccgccgac acgacgccgg cccggcccgt ggtcgcgccc
      721 cgcaccggcg gcggcgacga cgacgcgccc ccgcccccca ccgccaaacc gccgcgcgac
      781 cccgacccgc ccaagaagaa aggcaagaag ggttcggacg aaccgtagaa gaagagtcag
      841 aagtcagaac gcagaaggca gaactgaaaa accttttctt tcagttctgc cttctgcgtt
      901 tccgctacgt tctgacttct gcgttccgac ttctgacttt tacagcagcc tatccagcca
      961 gtacggcagc atcttgcgcc accagggcca gtcgtggttg acgtcgtggc cccagatgtc
     1021 gaggtggtgc gggacgcctt tccaggagag gatgccggag agctggcggc tgcgctcggg
     1081 cgcctcgtag gcgccctgcc ccgagaggat gacgatggcg tcggcctgcc gcaggcgcgg
     1141 caggaagtag ccgtcgtcga ggttctggag gtactggacg gggttgttga agtagacgtt
     1201 gtcgtcgtgg tagccgtcga accacgagcg cacgtcgtag ctgccgctca tggcgatggt
     1261 gccgcgcagg atgtccgggt gtttgaagta ggtgttggcc gcgaggtagg cgcccaggga
     1321 ggcgccggtc gtcatcggcc tttggtcgcc cgcgtcgttg cggatgagcg gcagcacctc
     1381 gtccgtgatg tagcggtcgt agcgcgtcaa cagctcgccc ttgatgtgcg gcggcgcctt
     1441 gtcgttcaag aggctgtagc ggttcacgct gttgatcgag taggcgcgca aacgcccggc
     1501 ctcgatgtgg tgcttgatcg agtccacgag gtagaaccgc tcgtattcga ggaagtcggc
     1561 ggcggcggtc gggaacatca ggagcggata gcccgagtgg ccgtaggcca ccagcggcat
     1621 ctccatcccc agattcgggc tgtaccaaga agtgatgcgg cgctccatcg cgaaaacacc
     1681 tccccgcgcc cctccggcgc ggcacgaact ctaaaagctg ttcgggattt cgagattacg
     1741 gcgacagata atctctcaaa cccgcccccc gggcaagccg gcgccccccg ggtgaccccc
     1801 ccgcg
//
LOCUS       DBNGOH010000689         4121 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_651559, whole genome shotgun sequence.
ACCESSION   DBNGOH010000689 DBNGOH010000000
VERSION     DBNGOH010000689.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4121)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4121)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4121
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_651559"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..444
                     /locus_tag="AC159P_13335"
     CDS             <1..444
                     /locus_tag="AC159P_13335"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024304.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HIR3696476.1"
                     /translation="GIVMLEIKDMSRGEMLALLLRVGYGHLGCARDGHPYIVPMHYAF
                     DSQDLYFFTTEGTKTEFISANHEVCFQVEEVTDALHWRSVMVTGRAKRLTEWDELERA
                     MQLITERNPTLTPALNKTEIGAWKRRNSIAVYRVRPEAFYGRTTI"
     gene            507..929
                     /locus_tag="AC159P_13340"
     CDS             507..929
                     /locus_tag="AC159P_13340"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024304.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HIR3696477.1"
                     /translation="MITRLNRDESLALLRGRRMGRLGCLAEGYPYVVPVNYAFDGESA
                     VSHSLPGRKIEAMRENPRVCLQVDDIEDQLNWRSVIAYGTYQEITGASDRARAINLLL
                     SLFPELTPVESLIAGDAGAPAPIVFRLKIDRVTGVREG"
     gene            1163..2089
                     /locus_tag="AC159P_13345"
     CDS             1163..2089
                     /locus_tag="AC159P_13345"
                     /inference="COORDINATES: protein motif:HMM:NF012791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR3696478.1"
                     /translation="MEVESILCPIDLSPESGRALRYARALAGAYGARLFVFHCAGGRA
                     EGEPIGVLEEVEFAVERALGPRDGAEGAEPEIVVVGGRDPAEAITREAAARGVGLIVM
                     CSRRRPLRAALLGSTAESVYRTAPCPVLITHPDENERADAANGPLSLRRVLVAHDFSD
                     YSELALRLALSLAQAHQAELHLLHVLAPPILGEPELAWSQATVEGSYHKAARGLKQAV
                     PGEAYLWSEVKTAVRWGKPYREVLAYAKEQDIDLVCMGANGAGFGMQALVGSNVDRVL
                     RQSPCPVLVARPLKPLFVPPEAVAAAGAVKES"
     gene            complement(2325..2999)
                     /locus_tag="AC159P_13350"
     CDS             complement(2325..2999)
                     /locus_tag="AC159P_13350"
                     /inference="COORDINATES: protein motif:HMM:NF016836.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HIR3696479.1"
                     /translation="MLRSGMMNDTQVREEVFRELWLDPRLKRADIGVRAHEGTVTLSG
                     EVAGTDELLAALEAARRADGVFAVVDEIKVRPANRPRADTDIVKAVCNALERNAVIPY
                     RRIQFAVSNGWVALMGRVDLPREREAAASLVRRLEGVRGVYNLIKVMPPEARTQNVRD
                     AIGAALKRHAEREAEGIDVSLREGTANLTGKVHTWAEKQVILGALKHAPGVERVNDQL
                     SIDPYF"
     gene            complement(3197..3454)
                     /locus_tag="AC159P_13355"
     CDS             complement(3197..3454)
                     /locus_tag="AC159P_13355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013681294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696480.1"
                     /translation="MKLLAVAVVVFLINLPFGYWRSRVPARSRQWFLAIHLPVPFVIA
                     LRVFSGLGWQLASFPVLVAAFFLGQYLGGKLPRLFTRQLLR"
     gene            complement(3485..>4121)
                     /locus_tag="AC159P_13360"
     CDS             complement(3485..>4121)
                     /locus_tag="AC159P_13360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HIR3696481.1"
                     /translation="DCHNRPTHVFQPPDRSVDDSLAAGKLDASLPYLKREAVALLSKS
                     YATTDEGLQSIADGLEKFYRDNYPELSSAKSDSIRGAVAEVGRIFQTYTFPEMKTDWQ
                     THPNNIGHFYSQGCFRCHDGEHVSNTGRVIRKECTLCHTTLDQTERGIKTPAQGGAFR
                     HPVALGTQGEFECAACHKGNRGFQHPVNLGDLSQFQCTDCHAGKVWTVSSR"
BASE COUNT          704 a         1334 c         1358 g          725 t
ORIGIN      
        1 ggtatcgtga tgctggaaat caaagacatg tcgcggggcg agatgctggc gttgctcttg
       61 cgcgtgggct atgggcattt ggggtgcgcg cgcgacgggc acccttacat cgtcccgatg
      121 cactacgcct tcgactcgca agacctctac ttcttcacga ccgaagggac gaagaccgag
      181 ttcatctcgg ccaaccacga ggtctgcttt caggtcgagg aggtgacgga cgcgctccac
      241 tggcgcagcg tgatggtcac aggtcgcgcg aagcgactga cagagtggga tgagcttgag
      301 cgcgcgatgc agctcatcac ggagcgcaac ccgacgctca cgcccgcgct caacaagacg
      361 gaaatcggcg cgtggaagcg ccgtaacagc atcgccgtct accgcgtccg ccccgaagcc
      421 ttctacgggc ggacgaccat ctgaaacccg tgcgcccgtg gcgcccttcg gcgagcgggc
      481 gcggcacgag ggcgtgtggg tgagccatga taaccagact taacagagac gaatcactcg
      541 cgctcctgcg cgggcgtcgg atggggcggc tcggctgcct cgccgaaggc tacccttacg
      601 tcgtccccgt caactacgca ttcgacggcg agagcgccgt cagtcactcg ctgcccggtc
      661 gtaagatcga ggcgatgcgc gagaacccgc gcgtctgtct ccaggtcgat gacatcgaag
      721 accagctcaa ctggcggagc gtgatcgctt acgggactta ccaggagatc accggcgcgt
      781 cggatcgcgc gcgcgcgata aacctcttgc tctcgctctt cccagaactg actcccgtcg
      841 agtcgctcat cgcgggggac gcaggcgccc ccgcgcccat cgtcttccgc ttgaagattg
      901 acagggtgac gggcgttcgc gaaggctgac tcataaggcc acgggacttt aacgagaagc
      961 ccacagccct gacctaacgc gcccgcgtaa ggccgcgcac acgttgcgaa attctccgca
     1021 ctaccccaca caggttcaca caattcacac cgtaaatggg cgataaatgc acgccccccg
     1081 tcagcccgcg gcggcatacg cattgcacga acactgttcg aggagggggc cgcccgtgac
     1141 gcggcttttc acgagcaaga tgatggaagt cgaaagcatc ctgtgcccca ttgatttgtc
     1201 gccggagtcc ggcagggcgc tgcgctacgc gagggcgctg gcgggcgctt acggcgcgcg
     1261 gctcttcgtc ttccactgcg cgggggggcg tgcggagggc gagccaatcg gcgttctcga
     1321 agaagtggag ttcgccgtcg agcgcgcgct cggcccgcgc gacggcgcgg agggagccga
     1381 gccggaaatc gttgtcgtcg gcggtcgtga ccccgccgag gccatcacgc gcgaagccgc
     1441 cgcgcgcgga gtcggtctca tcgtgatgtg ctccaggcga cggcctctgc gggcggcgct
     1501 gctcggctcg accgcggagt cggtctaccg caccgccccg tgccccgtgc tcatcacgca
     1561 cccggacgaa aatgaacgag cggacgcggc aaacggcccc ttgagcctga ggcgcgtgct
     1621 cgtcgcacac gacttctccg actactcgga gctggcgctc cgtctcgcgc tctcgctcgc
     1681 gcaggcacac caggccgaat tgcacctgct gcacgtcctc gcgcccccga tcctcggcga
     1741 gcctgagctg gcctggtcgc aggcgaccgt cgaggggagc tatcacaagg ctgcgcgcgg
     1801 cctcaagcag gccgtgccgg gtgaggctta cctctggtcg gaagtcaaga ccgccgtgcg
     1861 ctgggggaag ccttaccgcg aggtactcgc ctacgccaaa gaacaggaca tcgacctcgt
     1921 ctgcatgggc gcgaacggtg cggggttcgg catgcaggcg ctcgtcggct cgaatgttga
     1981 ccgcgtgctc agacagtcgc cgtgcccagt gctggtcgcg cgcccgctca agcctctctt
     2041 cgttccgccc gaagcagtcg cggcggcggg cgcggtgaag gagtcatgac cgattgcggc
     2101 aaccggtgcg tgcgcccgtc gccgtgggtt tcgtccggct tgttctgaca gtgctgttcg
     2161 agcttggtct cgggcgtttc gttcttggtc tttactggga caggccggcg tccgacgacg
     2221 acatccggcg gggcgggcta atgctgttcg ggttaaactt actgacgctt gcgcccctga
     2281 tcgcgccaag actgcgcgga ttgaagcagg atgtcgagcc ggacttaaaa gtacggatca
     2341 atgctcagtt ggtcgttgac cctctcgacg cccggcgcgt gtttgagcgc gccgagaatt
     2401 acctgcttct cggcccacgt gtgaactttg ccggtgaggt tcgccgtccc ctctcttaac
     2461 gacacgtcga tcccttcggc ctcacgttcc gcgtggcgct tcaaggccgc cccgatggcg
     2521 tcgcgcacgt tctgcgtcct cgcctccggc ggcatgacct tgatcaggtt gtacacgccg
     2581 cgcacgcctt caaggcgccg aacgagcgac gcggcggcct cgcgctcgcg cgggagatcg
     2641 actctgccca tgagcgccac ccagccgttc gacaccgcga actgtatgcg ccggtagggg
     2701 atcaccgcgt tccgctcaag cgcgttgcag acggccttga cgatgtccgt gtccgcgcgc
     2761 ggcctgttgg ctggtctgac tttgatctcg tccaccactg cgaaaactcc gtcggcgcgc
     2821 cgcgccgcct ctaacgcggc cagcaactcg tcggtgccgg caacctcgcc ggacagagtg
     2881 acggtgcctt catgcgcgcg cacgccgatg tcggctcgct tgagtcgcgg gtcgagccac
     2941 aactccctga agacctcttc ccgaacctgt gtgtcgttca tcatgccgct gcgtagcata
     3001 gttttcacct ccgtcggatc agcggcaggc gaccggttcg aagccacggc gcgcgcccct
     3061 tccgacatca tgaagcagag caacggatgt gccggagaac cttcgtccgg gaatccggca
     3121 tgaacctgcg cgcggcagcc tggcgaatgc ggaaagttac gcagcgtgcc ggacaaaaaa
     3181 ccgcagccgc gcttcatcat cgaaggagct ggcgggtgaa cagtctcggg agtttgccgc
     3241 cgaggtactg gcccaggaag aacgcggcga cgagcacggg gaacgaggcg agctgccagc
     3301 cgaggccgct gaagactcgg agtgcgatga cgaagggcac gggcaggtgg atggcgagga
     3361 accactgccg cgagcgcgcg ggaacgcgcg agcgccagta gccgaacggg aggtttatca
     3421 gaaagacgac gacggcgacc gctaacagct tcatcactca actcctcgaa tactccggac
     3481 cgtctcaacg gctgctgacc gtccacacct ttcccgcgtg acagtcggtg cactggaact
     3541 gcgagaggtc gccgaggttg accgggtgct gaaatccgcg attgcccttg tggcaggcgg
     3601 cgcactcgaa ctcgccctgt gtgccgagcg ccacggggtg gcggaacgcg ccgccctgcg
     3661 ccggcgtttt gattccgcgc tcggtttgat cgagcgtcgt gtggcaaagg gtgcactcct
     3721 ttctgatgac tcgcccggtg ttgctcacgt gctcgccgtc gtggcagcgg aagcagccct
     3781 gcgagtaaaa gtggccgatg ttgttcgggt gcgtctgcca gtcggtcttc atctcgggga
     3841 aggtgtaggt ctggaagatg cgccccacct cggccactgc cccccttatc gagtcgctct
     3901 tggccgaaga caattccggg tagttgtcgc ggtagaactt ctccagcccg tcggcgattg
     3961 actgaagccc ttcgtcggtc gtcgcgtaag acttcgagag cagggcgacc gcttcacgct
     4021 tgaggtaggg gagcgacgcg tcgagcttgc ccgcggcgag cgagtcatcg acggagcggt
     4081 cgggcggctg gaagacgtgt gtcgggcggt tgtggcagtc g
//
LOCUS       DBNGOH010000690         3078 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4501305, whole genome shotgun sequence.
ACCESSION   DBNGOH010000690 DBNGOH010000000
VERSION     DBNGOH010000690.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3078)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3078)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3078
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4501305"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1106
                     /locus_tag="AC159P_13365"
     CDS             <1..1106
                     /locus_tag="AC159P_13365"
                     /inference="COORDINATES: protein motif:HMM:NF024948.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HIR3696482.1"
                     /translation="PSPPGRGVRAARRAASLLLLPASLLLCAHLSAARASAATPLAVY
                     RDKVREAAVLTDELVTFCENDAHSGSSGPAAEESKYLANVRALLPPSETVERGGTTTV
                     VNNSWLHAALDEYEKMPAGAPSDNLKRARRIEGVGDRLRALDARLAETEAQPSTPRDK
                     DAEKGRLATILRGKEYNREVAEGGAIQRLIKRIRDWLADLVPDMRPIKPGAGRPSRLV
                     QYLVFALALAVILYIVWRYWVRRRDRIRPVAPREARVVLGERIEADRTASDLLDEAEL
                     LAREGELRGAIRKAYVALLCELGDRKVIRLAQHKTNRDYLRAVRKDAPPLLYEQMQPL
                     TNNFERHWYGLEEADQSDWADFRTRCRQALTKV"
     gene            1181..2686
                     /locus_tag="AC159P_13370"
     CDS             1181..2686
                     /locus_tag="AC159P_13370"
                     /inference="COORDINATES: protein motif:HMM:NF025617.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4350 domain-containing protein"
                     /protein_id="HIR3696483.1"
                     /translation="MKGRLAIFITVAVMLVILVALNAASYVRVEDEVLSEFNPDRSTF
                     NADATGTRAFYTFLEQSGHQVSRWGRPPAALLAGASAPANFVVVGKTRVEVESEDAEA
                     ILKWVEGGGRLVIIDRVPPASLVPAAGGWRVSAEITEYPGPEVRPDNTETMTRGVPLI
                     TPSQPTWLVRDVAQVTRSRFAGRLHVYPFIAAPQTVGVSGPVPTPTIDDDEPWFEEEP
                     PPPPPKPAPSPVAVVSDAAEDEDEDEDAEEPSETVESPAPVEHLADGREGTGSLLIDY
                     PYGRGRVVILSDPFIVSNGGVKLADNLHLAANVVAGAGGPIAFDEYHHGHGATENPVL
                     AYFTGTPVLWIFAQAGLIVLAVLWTRGRRFARPLPAPHVDRRSKLEFVASMAELQARA
                     RAYDLAVENVYARTRRARSSPSASPPARAATRAGSKRSSPTARTPSPARPPRRARPSP
                     SSARCANSNATSASSCARAKSGSHDKEFRIPDSGLFISRLLCFDTATELSD"
     gene            2723..>3078
                     /locus_tag="AC159P_13375"
     CDS             2723..>3078
                     /locus_tag="AC159P_13375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003442736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR3696484.1"
                     /translation="MLHTVPSTVEFIRGELAKVIVGQDEVIEQVLVALLAEGHALIEG
                     VPGTAKTLMVKTLARIVGADFSRIQFTPDLMPSDITGTNVFNPATAAFTLRRGPVFTD
                     ILLADEINRTPPKTQAA"
BASE COUNT          486 a         1142 c         1009 g          441 t
ORIGIN      
        1 aaccctcgcc tccggggcgc ggtgtgcggg ccgcacgtcg cgccgcgtcg ctgctcctgc
       61 tgccggcgtc gctgttgttg tgcgcacacc tgtcggcggc gcgcgcgtcc gccgccaccc
      121 cgctcgccgt ctaccgcgat aaggtgcggg aggcggcggt gctgaccgac gaacttgtca
      181 cgttctgcga gaatgacgcg cactcgggat cgagcggccc cgccgcggag gagagcaagt
      241 acctcgcgaa cgtgcgcgcg ctgctcccgc cgtccgagac ggtcgaacgg ggcggcacga
      301 cgaccgtcgt caacaactcg tggctgcacg ccgcgctcga cgaatacgag aagatgccgg
      361 cgggcgcgcc ctccgacaac ctcaagcgcg cacggaggat cgagggcgtc ggcgaccgcc
      421 tgcgcgcgct cgacgcgcgc ctcgccgaga ccgaagcgca gccctcgacg ccgcgcgaca
      481 aggacgccga gaaggggcgg ctggcgacca tcctgcgcgg caaggagtac aaccgcgagg
      541 tggccgaggg cggcgcgatc cagcgcctca tcaagcgcat ccgcgactgg ctcgccgacc
      601 tcgtgccgga catgaggccg atcaagcccg gcgccggtcg gccctcgcgc ctcgtccagt
      661 acctcgtctt cgcgctcgcg ctcgccgtca tcctctacat cgtctggagg tactgggtgc
      721 gccgccgcga ccgcatccgg cccgtcgcgc cgagggaggc gcgcgtcgtc ctcggcgagc
      781 gcatcgaggc ggaccggacg gcctccgacc tgctcgacga ggccgagctg ctggcgcgcg
      841 agggcgagct gcgcggcgcc atccgcaaag cctacgtcgc gctgctctgc gagctgggcg
      901 accgcaaggt tatccgcctc gcgcagcaca agaccaaccg cgactacctg cgcgccgtgc
      961 gcaaagacgc gccgcccctg ctctacgaac agatgcagcc gctcaccaac aacttcgagc
     1021 gccactggta cggcctcgaa gaggccgacc agtccgactg ggccgacttc cgcacgcgct
     1081 gccgtcaagc gttgaccaaa gtctgaagtc ggaagtctga agtcggaaaa cgattcttcg
     1141 gttcagactt cagacttcag acttcagact tcagactttc atgaagggcc gcctagccat
     1201 cttcatcacc gtggcggtga tgctggtcat cctcgtcgcg ctcaacgccg cctcctacgt
     1261 gcgcgtcgag gacgaggtgc tgtcggagtt caaccccgac cgctcgacct tcaacgcgga
     1321 cgcgacgggg acgcgcgcct tctacacctt cctcgaacag tccggccatc aggtgtcgcg
     1381 ctggggtcgc ccccctgcgg cgctgctcgc gggcgcgtcg gcgcccgcga acttcgtcgt
     1441 cgtcgggaag acgcgcgtcg aggtcgagag cgaggacgcc gaggcgatcc tgaagtgggt
     1501 cgagggcggc ggtcgcctcg tcatcatcga ccgcgtgccg ccggcgagcc tcgtgcccgc
     1561 ggcgggcggc tggcgcgtgt cggcggagat cacggagtac cccggccccg aagtgcggcc
     1621 cgacaacacc gagacgatga cgcgcggcgt gccgctcatc acgccgtcgc agccgacctg
     1681 gctcgtgcgc gacgtggcgc aggtgacgcg ctcgcgcttc gccggtcgtc tccacgtcta
     1741 tccgttcatc gccgcgccgc agacggtggg cgttagcggc cccgtgccga cgcccaccat
     1801 cgatgacgac gagccgtggt tcgaggaaga gccgccgccc ccgccgccga agccggcccc
     1861 ttcgcccgtc gccgtcgtca gcgacgcggc ggaggacgag gacgaggacg aggacgccga
     1921 agagccgagc gagacggtcg agtcgcccgc gccggtcgag cacctcgcgg acgggcgcga
     1981 aggcacaggc tctctgctca tcgactaccc ttacgggcgc gggcgcgtcg tcatcctgag
     2041 cgacccgttc atcgtctcga acggcggcgt caagctcgcc gacaaccttc atctcgccgc
     2101 gaacgtcgtc gcgggcgcgg gcggccccat tgccttcgac gaataccacc acggccacgg
     2161 cgcgaccgag aaccccgtcc tcgcctactt cacaggcacg cccgtcctct ggattttcgc
     2221 gcaagccggc ctcatcgtcc tcgccgtcct ctggacgcgc gggcgcaggt tcgcgcgccc
     2281 gctcccggcg ccgcacgtgg atcgccgctc gaagctggag ttcgtcgcct cgatggccga
     2341 gctacaggcg cgcgcgcgcg cctacgacct cgccgtcgag aacgtctacg cccgcacgcg
     2401 ccgcgcgcgc agctcgccga gcgcgtcgcc gcccgctcgg gccgcgacgc gcgccggctc
     2461 gaagcgctcc tcgccgactg cgaggacgcc atcgccggcg cgcccacctc ggcgcgcaag
     2521 gccgtcgccc tcgtccgcgc gctgcgcgaa ctcgaacgcg acctcggcat cctcatgcgc
     2581 gcgcgcgaag tcaggcagtc acgataaaga atttcggatt ccggattccg gattgttcat
     2641 ttcccggctt ctgtgctttg ataccgccac tgagctaagt gattaaatcc aaaatcggaa
     2701 agccgaaatc caaaacccga acatgcttca cacagtcccc tccacggtcg agttcatccg
     2761 cggcgagttg gcgaaggtca tcgtcgggca ggacgaggtg atcgagcagg tgctcgtggc
     2821 gctcttggcc gaagggcacg cgctcatcga gggcgtgccg ggcacggcga agacgctgat
     2881 ggtgaagacg ctggcgcgca tcgtcggcgc cgacttcagc cgcatccagt tcacgcccga
     2941 cctgatgccc tcggacatca cgggcacgaa cgtcttcaac ccggcgaccg ccgccttcac
     3001 gctgcgccgc ggccccgtct tcaccgacat cctgctcgcc gacgagatca accgcacgcc
     3061 gccgaagacg caggccgc
//
LOCUS       DBNGOH010000691         2023 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3953230, whole genome shotgun sequence.
ACCESSION   DBNGOH010000691 DBNGOH010000000
VERSION     DBNGOH010000691.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2023)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2023)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2023
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3953230"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..972)
                     /locus_tag="AC159P_13380"
     CDS             complement(<1..972)
                     /locus_tag="AC159P_13380"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HIR3696485.1"
                     /translation="MTTRKQSGGGVRRAGCRRVLLKASVVAVAVLVGLLICEVFLRAV
                     GYTYPVFYAPDPELGYVLRPGAAGWYRKEGAAYVRVNSQGLRDREHAREKPPGVVRVA
                     VVGDSYAEALQVAQEAAFWSVMERDLSGCGAFDGREVEVINFGVSGYGTAQEMLTLRR
                     KVWAYSPDIVLLAVTTNNDITDNSRALKRTDEVPYFVLRDGRLVLDDSFRTSRAFVLR
                     QSALNRAGRWVRDHVRVIQAIHQSHGAIKSAIAAWRAKRDAPAAAGASKKAGDARGDE
                     AKTTVVEEPGTDNLIYREPAGEVWHEAWRVTEALIARMREETESRGAK"
     gene            996..>2023
                     /locus_tag="AC159P_13385"
     CDS             996..>2023
                     /locus_tag="AC159P_13385"
                     /inference="COORDINATES: protein motif:HMM:NF016593.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HIR3696486.1"
                     /translation="MNRTSRQIGLELTADELVAALIRLAPERGACVLDSCGARGDGAR
                     FLVAGLDPSETVEARGEELRLRARGESAARVERGSVLSLLDARLDEFRVARDASSDLL
                     PARGACVAALSYDLARQFEKPRPRAPSPRAANTDPDAVLAFFDTLVVHDYARGVTTLV
                     GVGDAAERLEEARATLLGAAAGGDAAEALEAAPSCSANEESVSAKSDSMREALVAAGS
                     NLTRAEYVAAVERIREHIYAGDIYQANLTQQISCATPGGATPERVFLRLRREHPAAFA
                     AFVRLPGGGAVVSASPERFLRVRFEVAGPDAARVVEAWPIKGTRRRGRDAAEDARLRA
                     ELLSSAKD"
BASE COUNT          284 a          733 c          716 g          290 t
ORIGIN      
        1 cttcgcgccc ctgctttcgg tctcctcgcg catccgcgcg atcaacgcct cggtcacgcg
       61 ccacgcctcg tgccagacct cgcccgcagg ctcgcggtag atgaggttgt ccgtgcccgg
      121 ctcttcgacg acggtcgtct tcgcttcgtc gccccgcgcg tcgcccgcct tcttcgacgc
      181 gcccgccgcc gcgggcgcgt cgcgcttcgc acgccacgcg gcaatcgcgg acttgatcgc
      241 gccgtgcgac tggtggatgg cctgtatgac gcggacgtgg tcgcgcaccc agcggcccgc
      301 gcggttcagc gccgactggc gcaagacgaa agcgcgcgaa gtgcggaacg agtcgtcgag
      361 cacgagccgc ccgtcgcgca ggacgaagta gggcacctcg tccgtccgct tcagcgcgcg
      421 cgagttgtcc gtgatgtcgt tgttggtcgt gacggcgagc agcacgatgt cgggcgagta
      481 ggcccagacc ttccgccgga gcgtcagcat ctcctgcgcc gtcccgtagc cggagacgcc
      541 gaagttgatg acttcgacct cgcgaccatc gaacgcgccg cagccgctga ggtcgcgctc
      601 catcacagac cagaaggccg cctcttgcgc gacctgcaaa gcctccgcgt aagagtcgcc
      661 gacgacggcg acgcggacga cgccgggcgg cttctcgcgc gcgtgctcgc ggtcgcgtaa
      721 gccctgcgag ttgacgcgga cgtaagcggc gccctccttc cgataccagc ccgcggcgcc
      781 gggtctgagc acgtagccca gctccgggtc gggcgcgtag aagaccgggt acgtgtagcc
      841 gacggcgcgc aggaagacct cgcagatcag cagcccgacc agcacggcca cggccacgac
      901 cgacgccttc aacaaaacgc ggcggcaccc cgcgcgccgc acgcccccgc cactttgctt
      961 tcgcgtcgtc actgcctgat gatgccgcct gacgtgtgaa tcggacaagc agacagatcg
     1021 gacttgagct gacggcggac gaactcgtcg ccgcgctcat ccggctcgcg ccggagcggg
     1081 gcgcctgcgt cctcgacagt tgcggggcgc gcggcgacgg cgcgcgcttc ctcgtcgcgg
     1141 ggctagaccc ctccgagact gtcgaggcgc gcggcgaaga gctgcgcctg cgtgcgcgcg
     1201 gcgagtcggc ggcgcgcgtc gagcgcgggt cggtgctgtc gctgctcgac gcgcggctgg
     1261 atgaattccg cgtcgcgcgg gatgcgtctt ccgaccttct acccgcgcgc ggcgcgtgcg
     1321 tcgcggcgct ctcttacgac ctcgcgcggc agttcgagaa accgcgcccg cgcgctccgt
     1381 ctccgcgcgc cgccaacaca gaccccgacg ccgtgctcgc cttcttcgac acgctcgtcg
     1441 tccacgatta cgcgcgcggc gtgacgacgc tggtcggcgt cggcgacgcg gcggagcggc
     1501 tcgaagaagc gcgcgcgact ctgctcggcg cggcggcggg cggcgacgcc gcggaagcac
     1561 tcgaagcggc gccttcatgt tccgcgaacg aagaaagcgt cagcgcgaag tcggactcga
     1621 tgcgagaagc gctcgtcgcc gccgggtcga acctgacgcg cgcggagtat gtcgccgccg
     1681 tcgagagaat cagggagcac atctatgcgg gcgacatcta tcaggcgaac ctcacgcagc
     1741 aaatctcctg cgcgacgccg gggggcgcga cgcccgagcg cgtcttcctg cgactgcggc
     1801 gcgagcaccc tgcggccttc gccgccttcg ttcggctgcc ggggggcggc gccgtcgtca
     1861 gcgcgtcgcc cgagaggttc ctccgcgtgc gcttcgaagt ggcggggccg gacgcggccc
     1921 gcgtcgtcga ggcgtggccg atcaagggca cgcggcggcg cgggcgcgac gcggcggagg
     1981 acgcgcggct gcgcgcggag cttttgtcga gcgcgaagga cag
//
LOCUS       DBNGOH010000692         2407 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1204106, whole genome shotgun sequence.
ACCESSION   DBNGOH010000692 DBNGOH010000000
VERSION     DBNGOH010000692.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2407)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1204106"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..902
                     /locus_tag="AC159P_13390"
     CDS             <1..902
                     /locus_tag="AC159P_13390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020706891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696487.1"
                     /translation="LKEHGSRVRGTYDILREALAEVNRDPAGLLRAVKQADEEAAALG
                     ATYDPARRVALSVRLTDRPTEKLLKGLEYRTEQSDVSGQLRVVWGTKPLDLKVPFYEE
                     AEPSVSVAPPLYYLVPPQWKQVAEVLAAHGLRLKRLARPATVEVESYRFREVKFNPSS
                     FEGRVLVTFKTEPVREKRTYPAGTVVVPVAQAASRVALHLLEPDAPDSFVTWGFFHPV
                     FEQKEAGEDYVLEKLAREMLAKDASLKREFEQRLASDAKFAASAGERLRFFYERSPYF
                     DRQMNLYPVGRVTTRLDAPLNEF"
     gene            complement(1117..1263)
                     /locus_tag="AC159P_13395"
     CDS             complement(1117..1263)
                     /locus_tag="AC159P_13395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008478596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lmo0937 family membrane protein"
                     /protein_id="HIR3696488.1"
                     /translation="MLWTILVVLLVLWLLGLIGGVGGSLIHLILVVALVILVINLLTG
                     RRTV"
     gene            1865..>2407
                     /locus_tag="AC159P_13400"
     CDS             1865..>2407
                     /locus_tag="AC159P_13400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696489.1"
                     /translation="MPPLPRLLPRTQKQNVAVAASILTLAAVIAFAGAKLFFGPTPPA
                     VETINYTRLREIAEASEAASVKIDGELVTITGKAGVVSQAIVDKAAQQHVADTFARNK
                     VPLEYSSTRPNAFERTMNYAVPLVVLLALGVIGWRVYASMGGGGGGLQLADTTSVQDV
                     TFADVAGVDEAREELSETIDF"
BASE COUNT          454 a          764 c          760 g          429 t
ORIGIN      
        1 tgctcaagga gcacggctcg cgcgtgcgcg gcacgtacga catcctgcgc gaggcgcttg
       61 ccgaggtgaa ccgcgacccc gcgggcctgc tccgcgccgt gaagcaggcg gacgaagagg
      121 cggcggcgct tggcgcgacg tacgacccgg cgcgacgcgt ggccttgagc gtgaggctga
      181 ccgacaggcc gaccgagaag ctgttgaagg gtctcgaata ccgcacggag cagagcgacg
      241 tgtccgggca gttgcgcgtc gtgtggggaa cgaagccgct cgacttgaag gtgcctttct
      301 acgaagaggc ggagccgtcc gtgtcggtcg cgccgccgct ttattatctc gtgccgccgc
      361 agtggaagca ggtcgcggaa gtgctcgcgg cgcacgggct gcggctgaag cgtctggcgc
      421 ggcccgcgac cgtggaggtc gagagctacc gcttcaggga ggtgaagttc aacccctctt
      481 cgttcgaggg gcgcgtgctg gtcactttca aaacggagcc ggtgagggag aagaggactt
      541 accccgcggg cactgtggtc gtgcccgtgg cgcaggcggc ctcgcgcgtc gcgctccacc
      601 tgcttgagcc tgacgcgccc gactcgttcg tcacgtgggg cttcttccac cccgtcttcg
      661 agcagaaaga ggcgggcgaa gattacgtgc tggaaaagct cgcgcgcgag atgctggcga
      721 aggacgcgag cctgaagcgc gagttcgagc agcgcctcgc gtccgacgcg aagttcgcgg
      781 cgagcgcggg cgagcggctg cgcttcttct acgaacgctc gccctacttc gacaggcaga
      841 tgaacctcta tccggtcgga cgcgtgacga cgagactgga cgcgccgctg aatgaatttt
      901 agaacgtccg gcaggactgt aggggcaggg cttgtccgcg gcccgtcaag atggaattga
      961 ttcaacgtcg cgtgtgacgc gagcgcgggc cgcggacttg ccctgcccct acaaacgtca
     1021 tcacgagtct taaaaacaga ccgtcggcct gctggaaaga aagggcggcg aaccgatgtc
     1081 cgccgccctc cgtgcgaaaa ttcctcaccg cttccgttag acggtgcgcc gccctgtcag
     1141 caggttgatc acgaggatca cgagggccac gacgagaatc aggtgaatca agctgccacc
     1201 aaccccgccg ataagcccga gcagccagag cactaagagc accaccagaa ttgtccacaa
     1261 catatcgcca tctccttttc gagcgtcagt agttttacca ttacggttcg cttgaacccg
     1321 actacttctc ctttgagcaa cgcccatgcc agtggccgcc cgtggccgca gggggcgggc
     1381 aagtctttga ttaagcggga tttattcgga agtccgacga agcttcggca gaaagtgttg
     1441 aaccgtcttg tatggaaaca cgccgtcaaa cttacccgcc gcgtgttttg atacaagcca
     1501 cgcccgattt ttgtaaatct ttcgacacag gtgcggcaac tgagagcctg tgtccgacat
     1561 ccgactggcg gcactgaaac tgttctccgc caagcaagca gctcattcat aaacggcaat
     1621 cgtccgctca gcttttctgc tggacttcgt cgtcggaagg ctgatagatt ggaaatgcat
     1681 cggtcgggta attcacgccc gccgaggcgc gagtgttcgg ttcacctccg gtgagtcctt
     1741 ccgccgaagc ttcagggctt ctccttcgga cacggtttct ttgcaccgct aataatcaaa
     1801 caggtcgcgg gctgccgcct tctcccccga actcgaaggc cgctcgcccg acgagaggtt
     1861 cgttatgccg ccgttaccac gtctgcttcc ccgtacgcaa aaacagaacg tcgccgtcgc
     1921 cgccagcatc ctgaccctcg ccgccgtcat cgctttcgcc ggcgccaaac tcttcttcgg
     1981 gccgacgccg cccgccgtcg agaccatcaa ctacacgcgc ctgcgcgaga tcgccgaagc
     2041 gtccgaagcc gcctcggtca agatcgacgg cgagctggtg accatcaccg gcaaggccgg
     2101 cgtcgtctcg caggccatcg tggacaaggc cgcgcagcag cacgtcgcgg acaccttcgc
     2161 gcgtaacaaa gtgccgctcg aatacagttc gacgcgcccg aacgccttcg agcggacgat
     2221 gaactacgcc gtccctctcg tcgtgcttct ggcgctcggc gtcatcggct ggcgcgtcta
     2281 cgcgagcatg ggcggcggcg gcggcgggct gcaactggcc gacacgacca gcgtgcagga
     2341 cgtgacgttc gcggacgtgg cgggcgttga cgaggcgcgc gaagaactct cggagacgat
     2401 cgatttc
//
LOCUS       DBNGOH010000693         1699 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1204174, whole genome shotgun sequence.
ACCESSION   DBNGOH010000693 DBNGOH010000000
VERSION     DBNGOH010000693.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1699)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1699)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1699
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1204174"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..349
                     /locus_tag="AC159P_13405"
     CDS             <1..349
                     /locus_tag="AC159P_13405"
                     /inference="COORDINATES: protein motif:HMM:NF014658.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MqnA/MqnD/SBP family protein"
                     /protein_id="HIR3696490.1"
                     /translation="GLRVYAMAARGREPTGLGFVFALWMTRGDAGAAALSIDFAAARD
                     EGLASVEEIVASYERELRLPREELRSYLLDNICFHLDEEMRAGLELYFRLAHKHGIIE
                     HVRPLKTVGTQAA"
     gene            495..1643
                     /gene="mqnC"
                     /locus_tag="AC159P_13410"
     CDS             495..1643
                     /gene="mqnC"
                     /locus_tag="AC159P_13410"
                     /EC_number="1.21.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009096340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic dehypoxanthinyl futalosine synthase"
                     /protein_id="HIR3696491.1"
                     /translation="MTTTDIQPILDRVLAGGRMTAEECAALLGSHDVARMGVAADEIR
                     RRKHPSGVVTYIIDRNVNYTNVCNVVCTFCAFYRRPGKPDTYVQTVEQICEKIDETIA
                     LGGTGVLMQGGLHPDFGIEWYEDLLRDLHARYPDFQLHCFSPPEIHNIHLISKLSYEE
                     IMTRLKAAGLYSLPGGGGEILDDEVRKRVSTKCTTDEWLAVMRAVHRVGLRSTATMMF
                     GIGDRVEHRVRHLERVRDLQDETGGFTAFIPWTFQRENTALGRRIMEEPTGMDYLKML
                     AVSRLFLDNVEHIQSSWLTQGLRLGQVALRFGADDMGSIMIEENVVSAAGAHNTADER
                     MLRYLIREAGFTPQQRDILYRHVHRDESAKQDASDSASLRELTVAFAD"
BASE COUNT          309 a          533 c          574 g          283 t
ORIGIN      
        1 gggcttgcgc gtctacgcca tggcggcgcg ggggcgcgag cccacgggcc tcggcttcgt
       61 cttcgcgttg tggatgacgc gcggcgacgc aggcgccgcg gcgctctcca tcgacttcgc
      121 cgccgcgcgc gacgaggggc tggcgagcgt cgaagagatc gtcgcgtcgt acgagcgcga
      181 gctgcggctg ccgcgcgagg agctgcggtc ttacctgctc gacaacatct gcttccacct
      241 cgacgaggag atgcgggcgg gcctcgaact gtacttccgc ctcgcgcaca agcacggcat
      301 catcgagcat gtgcgcccgt tgaaaacggt tggcacgcag gctgcttaga ttgagtttgc
      361 gacgcggcgc gcacgacgct ttttggtttc gcggcgcttg acgcggaaca ttttcaagac
      421 ggtcaatcgc agattgcgga ttcgaaattc cgaatctcaa aatgcgaatc gaagtttgcc
      481 cggtgaggct ttgaatgacg acgaccgaca tccaaccgat tctcgaccgc gtgctcgcgg
      541 gcgggcgcat gacggcggag gagtgcgcgg cgctgctcgg gtcgcacgac gtggcgcgga
      601 tgggcgtggc ggcggacgaa atccgcaggc gcaagcaccc gtcgggcgtc gtgacgtaca
      661 tcatcgaccg caacgtgaac tacacgaacg tgtgcaacgt ggtctgcacg ttctgcgcct
      721 tctaccggcg gcccggcaag ccggacacgt acgtccagac cgtcgagcag atttgcgaga
      781 agatcgacga gaccatcgcg ctcggcggca cgggcgtcct catgcagggc gggttgcacc
      841 ccgatttcgg catcgagtgg tacgaagacc tgctgcgcga cctgcacgcg cgctaccccg
      901 acttccagct tcactgcttc tcgccgcccg agattcataa catccacctc atctcgaagc
      961 tctcgtatga agagatcatg acgcgcctca aggcggcggg cctctattcc ttgccgggcg
     1021 gcggcggcga gattctggac gacgaggtgc gcaagcgcgt ctcgacgaag tgcacgaccg
     1081 acgagtggct ggcggtgatg cgcgcggtgc accgcgtcgg tttgcgctcg acggcgacga
     1141 tgatgttcgg catcggcgac cgcgtcgagc accgcgtccg gcacctcgaa cgcgtccgcg
     1201 atttgcagga cgagacgggc ggcttcacgg ccttcatccc gtggacgttc cagcgcgaga
     1261 acaccgcgct cgggcgccgc atcatggaag agccgaccgg catggactat ctgaagatgc
     1321 tcgcggtctc gcgcctcttc ctcgacaacg tcgagcacat acagtcttcg tggctgacgc
     1381 aagggctgcg gctcgggcag gtggctctca ggttcggcgc cgacgacatg ggttcgatca
     1441 tgatcgagga gaacgtcgtc tccgccgcgg gcgcgcacaa caccgccgac gagcggatgc
     1501 tgcgctacct catccgcgag gcgggcttca cgccgcagca gcgcgacatc ctctaccgcc
     1561 acgtccaccg cgacgagtcg gcgaagcagg acgcgagcga ttcggccagc ctgcgcgagt
     1621 tgaccgtcgc gttcgcggac tgaaggagga agggcgaaaa ggttaaaagg ggaaaaggcg
     1681 aaaagggaaa gaggttgtc
//
LOCUS       DBNGOH010000694        10279 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3585055, whole genome shotgun sequence.
ACCESSION   DBNGOH010000694 DBNGOH010000000
VERSION     DBNGOH010000694.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 10279)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10279)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10279
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3585055"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..613)
                     /locus_tag="AC159P_13415"
     CDS             complement(<1..613)
                     /locus_tag="AC159P_13415"
                     /inference="COORDINATES: protein motif:HMM:NF019042.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HIR3696492.1"
                     /translation="MERKLAEATRRIEQLASAVESLLAEVSRLKGAQPGEATASQPTT
                     AASSQNDSDDPAAVFIERIVEPELGGSVRAEKLRASPEVFVQARYSALPVRGAGAEFE
                     PNLSLTRIETRWAGRVSERLGAGVEIQFHPALDGSPEELVNDAFIEYYLNDHTTVRVG
                     QFVKPFGFDIQQSSAVRESPERAVFAGYFFPGQRDRGLLLSGDL"
     gene            complement(789..1562)
                     /locus_tag="AC159P_13420"
     CDS             complement(789..1562)
                     /locus_tag="AC159P_13420"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HIR3696493.1"
                     /translation="MKRLTRREAVGAMVAGGTGLALANFTGSETASAEEGGGAIIVAQ
                     SQTPGAAARAFAGQHQPRPLPFNPAKLNGLSEKLIRSHHENNYGGAVKALNTVEQRLA
                     AMLGEKELPPYVYGDLKREELVRTGSVVLHENYFANLGGDGKAAGKVLDLIKHWFGSY
                     EQWEAEFKKTANALAGGSGWVILTHNMHTNELHNYWAWDHMHNAPMGRPLLVLDMYEH
                     AYHMDYGAAAAKYVDAFMQNVNWEEVNRRAEAALKSGPA"
     gene            complement(1582..2349)
                     /locus_tag="AC159P_13425"
     CDS             complement(1582..2349)
                     /locus_tag="AC159P_13425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="family 16 glycoside hydrolase"
                     /protein_id="HIR3696494.1"
                     /translation="MSRFNTAASTFALALLIVVACSGPGGMTDANADKSPPEANAVEP
                     EKPAPLQEAGKSFTYNFDSDAAGQVPAKFHTALTGQGAKGEWAVKADPSAPSQPNVLA
                     QTSADKTSYRFPLAVADEGSFRDLEMSVRFKPISGREDQAAGLVWRLKDANNYYIVRA
                     NALEGNVVLYKVQNGKRSDLPLKGEGRTYGKKTSVPSGQWSELRVVATGNLFEVFLGD
                     RKLYEVEDGTFPEAGKVGLWTKADSVTYFDDLRVTAK"
     gene            complement(2346..3809)
                     /locus_tag="AC159P_13430"
     CDS             complement(2346..3809)
                     /locus_tag="AC159P_13430"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3696495.1"
                     /translation="MNTRSLRFRLAVWYAGLLATLLALFGASVYFGLGRYLEWSLRES
                     LARQARQIGETYLADVRVSGEDYVVDEIKEHYAPEINGRYVRVTRGDGSVLFASGAPE
                     DGGFDPAGLPAPPAGINEALAREEHLAGGGELLIYALPFTARDGGRFLIETGAPYDQI
                     EGVLRGLLLTLAVGLPVMVCLAVAGGYVVVRRALRPVDALTRSAVRITSRNLGERLPV
                     AHTGDELERLSVSLNLMVARLDEAFTHVRRFTADASHELRTPLTVLHGELEEMARQPQ
                     LTPELSERVGSALEETERLSKIVESLLSISRLDAGEAHVEKAHFDLAELAGTMTEFLR
                     LLAEDKNISLSSRTRGPVGVEGDPARLKQVVVNLLDNAIKYTPEGGSVEVSVEASGNR
                     AVLQVSDSGVGIPGDALPHVFERFYRVDKARSRHLGGTGLGLAIVKSICAAHGGRVSV
                     ESEEGKGSRFRVELPLARNGAGEETGRAQSSDGRSNS"
     gene            complement(3806..4486)
                     /locus_tag="AC159P_13435"
     CDS             complement(3806..4486)
                     /locus_tag="AC159P_13435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal response regulator transcription
                     factor"
                     /protein_id="HIR3696496.1"
                     /translation="MRILLVEDEEKISGFVERGLAEAAFAVDVAGDGAEGLEMATAYQ
                     YDLIILDLLLPGLSGTEVLRRLRRTNSQVPVLVLTARDAVSDKVENFEAGADDYLTKP
                     FAFAELLVRVKALLRRGPVARESVLRVADLELDRLSQQVRRAGQRIELTAKEYALLEY
                     LMANTGRVLSRTMIVEHVWDQSFDGATNIVDVYVRHLRGKVDDPFERKLIKTVRGVGY
                     SVGDEDEG"
     gene            complement(4612..5223)
                     /locus_tag="AC159P_13440"
     CDS             complement(4612..5223)
                     /locus_tag="AC159P_13440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696497.1"
                     /translation="MTRRRYFGNPVKCLAVALTVLFGTLVGSEGVQARRQEGETDALL
                     GRLEDSKISLADGIRQAEKAYGRAVSAKFEMKGDKLVLSVYTAKEGLFDDAEHNVLME
                     LIGDATRPQWTPKAEVFEDRPHVARSAMHLTAMQLSKMTLADVIEWAAARRDGTVYSV
                     IPAIREGQIVFDVLVARPGGWSDRLMLDAITGKPFVGRAGPCG"
     gene            complement(5341..5778)
                     /locus_tag="AC159P_13445"
     CDS             complement(5341..5778)
                     /locus_tag="AC159P_13445"
                     /inference="COORDINATES: protein motif:HMM:NF021357.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate resistance protein ChrB
                     domain-containing protein"
                     /protein_id="HIR3696498.1"
                     /translation="MKWVTRKEIRVNRTATCWLIRKFLDPEAKFLFVPADEVAAVEAA
                     SGATGFDAPGATYPHRDAEGRCSFAALVHERLAHDPVLVEMARVVQAADFKDQVDEHP
                     AARGLVLISRGFPLVTRDDQETAERASFLYDAMYASVRVGLGS"
     gene            complement(5799..7025)
                     /locus_tag="AC159P_13450"
     CDS             complement(5799..7025)
                     /locus_tag="AC159P_13450"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3696499.1"
                     /translation="MATSKEQNLVLPHARTRRARAADYLGLERNVAVVSAGVFLLGLG
                     EELWKKFLPKYLEHLGAGPVAIGLFGTAKDFFDAVYQYPGGWLADRAGRRRAFLVFIG
                     MASVGYLVYLFSPSWPWVFVGLGFAMAWHSMASPAIFAVIGDALPKERRAMGFTLQSI
                     LKRVPMIVSPLFGGALIAAFGVSYGVRAGLVVTLVLALITVLIVRVINIPVEVGETAN
                     IRGVWRSFHVALKRLLVSDIIIRLCEGMADIFIILYVTNVLGVGLAQYGILVAIQMTT
                     SILVYIPAGKIADRIGRKPFVIATFVCFALFPLAVVMSTGFASLVVAFVIGGLREIGE
                     PSRKAMIVDFAEAHLRARTVGLYYLIRSLAIAPAAVIGGMLWKLAPQTPFVTAGLIGI
                     IGTIVFAATVEEQYAG"
     gene            complement(7049..7834)
                     /locus_tag="AC159P_13455"
     CDS             complement(7049..7834)
                     /locus_tag="AC159P_13455"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016041.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HIR3696500.1"
                     /translation="MQFWLSEQARLSALIILCALLWTLESVVPLYRYGRDHLPHAFPN
                     VALALLLVLTNLALSAGTARLAAFSNSHRVGLFHLLELLPLLALILGVAALDFFGYLA
                     HLLLHKSRLGWRFHRVHHSEMAVDVTTAFRQHPGETVWRILWQMAAVVVFGLPLWVVV
                     IYLTLSGLNAQLEHANIRLNDRVDGLLRLFVVTPNMHKTHHSRLRKETDSNYSNIFSF
                     WDRLCGTYTPRVDFKELRYGLKGFDAADRQTLGSLLKMPFVKV"
     gene            complement(7916..8350)
                     /locus_tag="AC159P_13460"
     CDS             complement(7916..8350)
                     /locus_tag="AC159P_13460"
                     /EC_number="2.8.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF013607.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102100 - mycothiol-arsenate ligase
                     activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate-mycothiol transferase ArsC"
                     /protein_id="HIR3696501.1"
                     /translation="MKQEQIIVFVCEHGSAKSVVAAAHFNRLAGERGLELRAISRGTD
                     PDAEIAPNAKAGLRADGLVTSERPPAKLVQADAAGVTRVVTFCELPEAYVTTARVERW
                     DGVPPVSEDYEKARDEIVGLIRRLLDELESGNQPAPGRDKPR"
     gene            complement(8360..9775)
                     /gene="chrA"
                     /locus_tag="AC159P_13465"
     CDS             complement(8360..9775)
                     /gene="chrA"
                     /locus_tag="AC159P_13465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006160119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HIR3696502.1"
                     /translation="MAAESEPAVARERELSPSPGGDTRTAGRSVSFAEAFRFWVKLGF
                     ISFGGPAGQIAIMHRELVERRRWLSEERFLHALNYCMLLPGPEAQQLAIYIGWLMHRT
                     WGGIVAGAFFVVPSIFILLALSYVYAAHGNLPAVAGVLSGFKPVVVAIVVEAVLKIGG
                     RALKRRAHYLIAAAAFVAIYFLHVPFPLIILAAAAVGLAGARFWPGVFAAAPKTKEAD
                     AKTEGEGGVTGEVPLVIDDHAPPPSHTLPSHGRSLKILSVGLALWLLPLAALVAWRGW
                     GSLHAQEYRFFTQAALVTFGGAYAVLAYVTQAANSYGWLMPGQAVDGLALAETTPGPL
                     IMVLQFVGFMAGWNNPQGLDQTTSAVVGALVTTYCTFLPCFIFIFLGAPYIEVLRGNK
                     NLTGALSGVTAAVVGVVLNLALVFGAAVIWPRGLEGGADWRAAVMSVAAFVALYRYKA
                     DVLWVVLAGGALGLAWTLLGA"
     gene            complement(9711..>10279)
                     /locus_tag="AC159P_13470"
     CDS             complement(9711..>10279)
                     /locus_tag="AC159P_13470"
                     /inference="COORDINATES: protein motif:HMM:NF021357.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chromate resistance protein ChrB
                     domain-containing protein"
                     /protein_id="HIR3696503.1"
                     /translation="RGGAAAQARATKDGALDRAQYQGRRWVTRRNLHIDRLASAWLIR
                     QFIDRRPRFHFVAEGERVEGGIPFDMFGAEFTHQGEDCTFETLIKRFGFGDDLALGEI
                     AEIVHDIDLKDDKFNRLEAAGLDGVVRGLGALLNDDRKLLRQSGAVFDGLYALLGKDT
                     PKDKGEKDGGRKRTGGRAGKRVKSKSRR"
BASE COUNT         1732 a         3471 c         3329 g         1747 t
ORIGIN      
        1 ggaggtctcc ggagagcagc aggccgcggt cgcgctgccc ggggaagaag tagccggcga
       61 agacggcccg ctcgggcgac tcgcgcacgg cgctcgactg ctggatgtcg aagccgaacg
      121 gcttgacgaa ctggcccacc cggacggtcg tgtgatcgtt caggtagtat tcgatgaagg
      181 cgtcgttgac cagctcttcg gggcttccgt cgagggccgg gtggaactga atctctaccc
      241 cggcgccgag tcgttcagac acgcggcccg cccatcgcgt ctcgatgcgc gtcaggctca
      301 ggttcggctc gaactccgcc cccgcgcccc tgacgggcag tgccgagtag cgcgcctgca
      361 cgaatacctc cggactggcg cggagcttct cggcgcgtac gcttccgccc aactccggct
      421 cgacgatgcg ttcgatgaag acggctgccg gatcgtcgga atcgttttga ctcgacgcgg
      481 cggtcgtggg ctgtgaggcc gtcgcctcgc cgggttgcgc gcctttaaga cgcgacactt
      541 ccgcgagtaa agactcgacg gcgctggcca actgctctat ccggcgcgtg gcctcggcca
      601 gcttacgctc caactccgcg actctttcct ccggggcgcg ctgctgtgcg cacgctacga
      661 ccgccagaga aaagaagatc gccaaggtgg atagtgcttt catcgagttg acctgttaag
      721 agtcgcggtt cgggtcaggc gctctcgcgc ggcacccgcc gtctacgcct taaacgactg
      781 cggccgtctt acgcggggcc ggacttcaac gccgcctccg cccggcggtt gacctcctcc
      841 cagttgacgt tctgcataaa agcatccacg tacttcgccg ccgccgcgcc gtagtccatg
      901 tggtaggcgt gctcgtacat gtcgagcacg agcaggggcc gtcccatcgg cgcgttatgc
      961 atgtggtccc acgcccagta gttgtgcagc tcattggtgt gcatgttatg cgtaaggatc
     1021 acccagccgg agccgcccgc cagggcgttc gcggtcttct tgaattccgc ttcccactgc
     1081 tcgtagctcc cgaaccagtg cttgatgagg tcgagcacct tgcccgccgc tttgccgtcg
     1141 ccgccgaggt tcgcgaagta gttctcgtgc aggacgaccg agcctgtgcg caccagctcc
     1201 tcgcgcttga ggtcgccgta cacgtagggc ggcagttctt tctcgccgag catcgccgcg
     1261 agtcgctgct cgacggtgtt gagggctttg actgcgcccc cgtagttgtt ttcgtggtgc
     1321 gagcggatga gcttctcgga aagtccgttg agcttggccg ggttgaacgg gagcggcctc
     1381 ggctggtgct gcccggcgaa ggcgcgcgcc gccgcgcccg gcgtttgact ttgagccacg
     1441 ataattgccc ctcccccttc ttccgcgctc gcggtttctg aacccgtgaa gttagccagc
     1501 gccagccccg tcccgccggc gaccatcgcc ccgacggcct cacgcctcgt cagcctcttc
     1561 atattctctc cctccgcccg tttatttcgc cgtgacccgc aggtcgtcga agtatgtcac
     1621 cgaatccgct ttagtccaca gacccacctt gcccgcctcc gggaacgttc cgtcttccac
     1681 ctcgtaaagc ttccggtcgc ccagaaagac ctcgaagagg ttgcccgtgg ctaccacgcg
     1741 cagctcgctc cactgccccg aaggcacgct cgttttctta ccgtaagtgc ggccctcgcc
     1801 tttgaggggc aggtcagacc gcttgccgtt ctggaccttg tagagcacga cgttgccttc
     1861 gagggcgttg gcgcggacga tgtagtagtt gttcgcatcc ttcaaacgcc acacgaggcc
     1921 cgcggcctga tcctcccggc cgctgatagg cttgaacctg acgctcatct ccaggtcgcg
     1981 gaacgagccc tcgtcggcaa cagcaagggg gaagcggtag ctggttttgt cggctgaagt
     2041 ctgggccagc acgttcggct gcgacggcgc agaagggtcg gctttgaccg cccattcgcc
     2101 cttcgccccc tgacccgtca gggccgtgtg aaacttcgcc ggcacctgcc ccgccgcgtc
     2161 cgagtcgaag ttgtaggtga agctctttcc cgcctcctgg aggggcgcgg gcttttcagg
     2221 ctcgaccgca ttcgcttcgg gtggtgactt gtcagcatta gcatctgtca tcccgcccgg
     2281 cccgctgcac gcgacgacga tcaggagcgc gagcgcgaac gtgctcgcgg ccgtgttgaa
     2341 cctactcatg agttactcct tccgtccgat gactgggccc gacccgtctc ttcgcccgcg
     2401 ccgttccggg cgagcggcag ctcgacgcgg aagcggctgc ccttgccttc ttcgctctcg
     2461 acgctgacgc gcccgccgtg cgccgcgcag atcgacttga cgatggcgag gccgagtccc
     2521 gtcccgccca ggtggcgcga gcgcgccttg tccacgcggt agaagcgttc gaagacgtgg
     2581 ggcagcgcgt cgccgggtat ccccacgccg ctgtccgaga cttggaggac ggccctgttg
     2641 ccgctggctt cgacgctcac ctcgaccgac ccgccctccg gggtgtactt gatcgcgttg
     2701 tccaggaggt tgaccaccac ctgcttgagg cgtgcggggt cgccttcaac cccgacaggc
     2761 cccctagtac ggctgctgag cgagatgttc ttgtcttcgg cgagcagacg taagaactcg
     2821 gtcatcgtcc cggccaattc cgccaggtcg aagtgcgcct tctcgacatg ggcttcgccg
     2881 gcgtcgagcc gcgagataga gaggaggctt tcgacgatct tcgacaggcg ctcggtctct
     2941 tcgagagcgc tgccgaccct ttcgctgagt tcgggcgtga gctggggctg ccgggccatc
     3001 tcttccagct cgccgtgcag cacggtaagc ggcgtccgca gctcgtgcga ggcgtcggcc
     3061 gtgaagcgcc ggacgtgcgt gaaagcttcg tcgagccgcg cgaccatcag gttgagcgag
     3121 accgacagcc gttcgagctc gtcgccggtg tgcgccacgg gcagcctctc gcccaggttg
     3181 cgcgacgtga tccgcaccgc gctccgcgtc agggcgtcca cgggccgcag cgcccgccgc
     3241 acgacgacgt agccgcccgc gaccgcgaga cagaccatga cgggcagccc gacggcgagc
     3301 gtgagcagaa gcccgcgcag caccccttcg atctggtcgt agggcgcgcc cgtctcgatg
     3361 aggaaccggc ccccgtcgcg cgccgtgaag ggcagcgcgt agatcaggag ttcgccgccg
     3421 cctgcaaggt gttcctcgcg cgctagcgcc tcattgattc ccgccggcgg cgcgggtagc
     3481 cccgccgggt cgaagccccc gtcctcgggc gcgcccgagg cgaagagcac gctcccgtcg
     3541 ccgcgtgtca cgcgcacgta ccggccgttg atctccggcg cgtagtgctc tttgatctcg
     3601 tctacgacgt aatcctcgcc gctcacccgc acgtccgcga gatacgtctc gccgatctgc
     3661 cgcgcctgcc tggcgagcga ctccctgagc gaccactcca ggtagcgtcc gagtccgaaa
     3721 tagacggacg cgccgaagag cgcgagcaac gtggcgagca gccccgcgta ccacactgcc
     3781 agccggaatc ggagcgagcg cgtgttcatc cctcgtcctc gtcgccgacc gagtagccga
     3841 cgccgcgcac cgtcttgatc agcttccgct cgaaggggtc gtccaccttc ccgcggaggt
     3901 ggcgcacgta cacgtcgacg atgttcgtcg cgccgtcgaa gctctggtcc cacacgtgct
     3961 cgacgatcat ggtgcgcgag agcacgcgcc ccgtgttcgc catcaggtat tcgaggagcg
     4021 cgtactcctt ggccgtcagc tcgattctct gcccggcccg cctgacttgt tgggagagcc
     4081 ggtcgagctc aaggtcggcg acgcggagca cgctctcgcg cgccaccggc ccgcgccgga
     4141 gcagcgcctt gaccctcacg agcagctcgg cgaaggcgaa cggcttggtc aggtagtcgt
     4201 ccgcgcccgc ctcgaagttc tccaccttgt cggagaccgc gtcgcgcgcc gtcaggacga
     4261 gcaccggcac ctgcgagttc gtgcgccgga ggcggcgcag gacttcagtg ccgctcagcc
     4321 ccggcagcag caggtcgagg atgatcaggt cgtactggta ggcggtcgcc atctccaggc
     4381 cctcggcccc gtcacccgcg acgtcgacgg cgaacgccgc ctccgccagc ccgcgctcga
     4441 cgaagcctga gatcttctcc tcatcctcga ccaaaaggat tcgcatggac ggcgagcttc
     4501 ctgaaggtcg gcggcgagtc gaggcgtctc ctcccggtcc ccgttcgccc cggccttccg
     4561 ccagcggtga acccgcgaca acttgttagc cgtccccggc cggggcgggg cttacccgca
     4621 ggggccggcc ctccccacga acggctttcc ggtgattgcg tcgagcatca gacgatcact
     4681 ccagcccccc ggcctcgcca cgaggacatc aaaaactatc tggccttccc tgatcgccgg
     4741 gatcaccgaa tagaccgtac cgtctcgccg ggcggccgcc cattcgatga catccgcgag
     4801 cgtcatttta gacaactgca tcgccgtgag gtgcatggcc gaacgggcga cgtgcggcct
     4861 gtcttcaaac acctcggcct tcggagtcca ctgcggccgg gtcgcgtcgc cgatcaactc
     4921 catcaggacg ttgtgttccg catcatcaaa aagaccctct ttcgccgtgt agacgctcag
     4981 caccagcttg tcgcccttca tctcgaattt cgccgagacg gcacgcccgt aagccttctc
     5041 cgcctgccgg atgccgtcgg cgagagaaat cttactgtct tcgagcctgc cgagtaaggc
     5101 gtcggtctcc ccctcctgac gccgggcctg cacgccctcg gagccgacga gcgtcccgaa
     5161 aaggacggtt agggcgacgg ccaaacactt gacggggttc ccaaagtagc gtcggcgggt
     5221 catgacgagt ttctccttta ctcgtctaat gtcgtcgcgg gcatacgcat agctgcccgg
     5281 ttcgcgggag acattaacga agtttgatta acgccggatg aagccaagat taaaatccat
     5341 tcatgaaccg aggccgaccc tgacactcgc gtacatggcg tcgtagagga aagacgcgcg
     5401 ctcggccgtc tcctgatcgt cgcgcgtgac gagcgggaaa ccccggctga tgagtacgag
     5461 cccgcgcgcc gccgggtgtt catcaacttg atccttgaag tcggccgctt ggacgacgcg
     5521 cgccatctcg acgaggacgg ggtcgtgggc cagcctctcg tgcacgagcg cggcgaagga
     5581 gcaccggcct tcggcgtccc tgtgcgggta ggtggcgccg ggtgcgtcga acccggtggc
     5641 cccgctcgcg gcctcgacgg cagcgacttc atccgccggc acgaagagga acttagcctc
     5701 cgggtcgagg aacttccgta tcagccagca ggtggcggtg cggttgacgc ggatttcttt
     5761 gcgcgtgacc cacttcaccc tgacacctcc gtttccactt accccgcata ctgctcctcg
     5821 accgtagcgg cgaagacgat agttccgatg attcctatga ggcctgcggt gacgaagggc
     5881 gtctgcgggg cgagcttcca gagcatcccg ccgattaccg ccgcgggggc gatggcgagg
     5941 ctccggatga ggtaatacag cccgaccgtg cgcgcccgga ggtgggcttc ggcgaagtcg
     6001 acgatcatcg ccttgcgcga cggctcgccg atctcgcgca agcccccgat aacgaacgcc
     6061 acaaccagcg aggcgaagcc cgtggacatc accacggcca agggaaagag tgcgaagcag
     6121 acgaaggtgg cgatcacgaa cggcttacgc ccgatgcggt cggcgatctt gcccgcaggg
     6181 atatagacca ggatggacgt ggtcatctga atcgccacca gtattccgta ctgcgcgagg
     6241 ccgacgccca ggacgttcgt cacatacagg atgatgaaga tgtcggccat gccttcgcac
     6301 agcctgatga tgatgtccga gacgagcagg cgtttgaggg cgacgtggaa cgagcgccag
     6361 acgccgcgga tgttggcggt ctcgccaacc tcgacgggga tgttaatgac ccgcacgatg
     6421 agcacggtga tgagcgccag aacgagcgtg acgacgagac ccgcacgcac gccgtaggag
     6481 acgccgaatg cggcgatgag cgcgccgccg aagagaggcg agacgatcat cggcacgcgc
     6541 ttcaggatgg actgcaaggt gaagcccatc gcgcgccgct ctttcggcag cgcgtcgccg
     6601 atgacggcga agatcgccgg actcgccatg ctgtgccagg ccatcgcgaa cccgaggccg
     6661 acgaagaccc acggccacga agggctgaac agatagacga ggtagccgac ggaggccatc
     6721 ccgatgaaga cgaggaaggc gcggcgtcgg cccgcgcggt cggcgagcca cccgccgggg
     6781 tactggtaaa cggcgtcgaa gaaatctttg gccgtcccga aaagcccgat cgcgacgggg
     6841 ccggcgccga gatgctccag gtacttcggc aggaacttct tccacaactc ctcgccgagg
     6901 cccagcagga agacgcccgc cgagacgacg gcgacgttgc gctccagccc caaataatcc
     6961 gcggcacgcg cgcggcgcgt tcgcgcgtgc gggagcacca ggttttgttc tttgctcgtc
     7021 gccaagcctt cgccctcttg ccggtcaatc acactttcac gaacggcatt ttcaacagac
     7081 ttcccaaggt ctgccggtcg gcggcgtcga agcctttgag tccgtaacgt agctccttga
     7141 agtcgacccg cggcgtgtag gtgccgcaga gcctgtccca gaaagagaag atgttggagt
     7201 agttcgagtc cgtctctttc cgcaggcggg agtgatgcgt cttgtgcatg ttcggggtga
     7261 cgacgaacag ccgcagcagg ccgtccaccc tgtcgttcaa cctgatgttc gcgtgttcga
     7321 gctgcgcgtt caggccggag agagtcaggt agatgacgac gacccagagg ggcaggccga
     7381 agacgacgac ggccgccatc tgccagagta tccgccacac tgtttccccc ggatgttggc
     7441 gaaaggcggt ggtcacgtca accgccatct cggagtgatg cacgcggtga aaccgccagc
     7501 ccagccgcga tttgtgtagc aggaggtgcg ccaggtagcc gaagaagtcc agtgcggcga
     7561 cgcctaagat caatgccaac aggggcaaca attcgaggag gtggaaaagg ccgacacggt
     7621 ggctgttgct gaaggccgcg aggcgggccg tgcccgcgga gagggccaga ttcgtcagaa
     7681 ccagcagcag agccagcgcg acgttcggga acgcgtgggg caggtggtcc ctcccgtacc
     7741 ggtataaagg gacgacggat tcgagcgtcc acaggagggc gcagaggatg atgagcgcgg
     7801 agagccgggc ctgttcggaa agccagaatt gcatgatcgt ctacctccga aacttgtgtg
     7861 acagctcttg cgggcgagcc atcacgcttt cagtcattga atcccggccc ctcggtcagc
     7921 gtggcttgtc tcggccgggc gccggctgat ttcccgactc cagctcatcc aggagacgcc
     7981 tgatgagccc gactatctcg tcgcgggctt tctcgtagtc ttcgctgacc ggcggcacgc
     8041 cgtcccaccg ctcgacgcgg gcggtcgtca cgtaggcttc gggcagctcg cagaacgtca
     8101 cgacgcgggt gaccccggcg gcgtccgctt gaactagctt cgccgggggg cgctcgctgg
     8161 tcacgaggcc gtcggccctc agtccggcct tggcattcgg cgcgatctct gcgtccgggt
     8221 cggtgccccg cgagatagcc cgcagctcta agccccgttc gccggcgagt cggttgaagt
     8281 gtgccgcagc gacgacgctc ttcgccgagc cgtgctcgca cacgaacacg ataatctgtt
     8341 cctgcttcac gactgctcct cacgcgccga gcaacgtcca cgcgaggccg agcgcgccgc
     8401 ccgcgaggac cacccataag acatcggcct tgtaacggta gagtgcgacg aaggcggcga
     8461 cgctcatcac ggcggcgcgc cagtccgccc cgccctcaag gccgcgtggc cagatcacgg
     8521 ccgctccgaa gaccagtgcg aggttgagca cgacgccgac cacggcggcg gtgacgccgc
     8581 tgagcgcgcc ggtcaggttc ttgttcccgc gcagcacctc gatgtagggc gcgcccagga
     8641 agatgaagat gaagcacggc aggaaggtgc aataggtggt gacgagcgcg cccacgacgg
     8701 cgctcgtcgt ctggtctaag ccctgcgggt tgttccagcc cgccatgaag ccgacgaact
     8761 gcaagaccat gatgagcggc cccggagtcg tctccgccag cgccagcccg tccaccgcct
     8821 gaccgggcat gagccagccg taagagttcg ctgcctgggt gacgtaggcg agcacggcgt
     8881 aggcgccgcc gaaggtgacg agcgccgcct gagtgaagaa gcggtactcc tgcgcgtgca
     8941 ggctccccca cccccgccac gcgacgagcg ccgcgagcgg cagcagccag agcgcgaggc
     9001 cgaccgagag tattttcagg gagcggccgt gagacgggag cgtatgcgac gggggcggcg
     9061 cgtggtcgtc tatgaccaga ggcacctcac ccgtgacccc gccttcgccc tcggtcttgg
     9121 cgtcggcttc tttcgtcttc ggggcggcgg cgaacacccc cggccagaag cgcgcgcccg
     9181 cgaggccgac ggccgccgcg gcgagtatta tcagcgggaa agggacgtgc aggaagtaga
     9241 tggcgacgaa ggcggcggcc gcgataaggt agtgcgcccg ccgcttgagg gcgcggcccc
     9301 cgatcttcag caccgcctcg acgacgatag cgaccacgac gggcttgaag ccggagagca
     9361 cgcccgccac cgcgggcaag ttgccgtgcg ccgcgtagac gtaggagagc gcgagcagga
     9421 tgaagatgga ggggacgacg aagaacgcgc cggcgacgat cccgccccac gtgcggtgca
     9481 tcagccagcc gatgtagatg gcgagttgct gcgcctccgg gccgggcagg agcatgcagt
     9541 agttcaaggc gtgcaggaac cgctcctccg agagccagcg gcggcgctcg accagctcgc
     9601 ggtgcatgat ggcgatctgc cctgccgggc cgccgaagga gatgaagccc agcttgaccc
     9661 agaagcggaa cgcctcggcg aaagagacac tccgcccggc agtccgggtg tcacctccgg
     9721 gacttggact taactctctt tcccgcgcga ccgccggttc gctttctgcc gccatccttt
     9781 tcccctttat ccttcggagt gtccttaccg agcagcgcgt acaagccgtc gaacacggcg
     9841 cccgactgcc ggaggagctt gcggtcgtcg ttgagcagtg ccccgaggcc gcggacgacg
     9901 ccgtccagcc ccgcggcttc gagccggttg aacttgtcgt ctttaagatc aatgtcgtgg
     9961 acgatctcgg cgatctcccc cagagcaagg tcgtcaccga acccgaaccg tttaattaaa
    10021 gtctcgaagg tgcagtcctc gccctggtgg gtgaactccg cgccgaacat atcgaagggg
    10081 atgccgccct cgacccgttc cccctcggcg acgaagtgga agcgcggccg cctgtcgatg
    10141 aactgcctga tcagccaggc ggaggccagg cggtcgatgt gcaggttgcg gcgcgtgacc
    10201 cagcgccggc cctggtactg cgcgcggtcc agcgcgccgt ctttcgtcgc gcgcgcctgc
    10261 gcggcagccc ccccccgcc
//
LOCUS       DBNGOH010000695         2600 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1754132, whole genome shotgun sequence.
ACCESSION   DBNGOH010000695 DBNGOH010000000
VERSION     DBNGOH010000695.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2600)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2600)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2600
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1754132"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1026)
                     /locus_tag="AC159P_13475"
     CDS             complement(<1..1026)
                     /locus_tag="AC159P_13475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696504.1"
                     /translation="MRKQIAVIALSAAVLAGGARAFAQTPAPPQQPAQGGDPAINANG
                     VLGEVVAVDVNARQIFVKTDAGSVVIVTLAEGVSYKKVPPGETSLLKATDIALKDIAT
                     GDRVFARGKVSDDRKAVMPRQVIVMSKGDIEQKREAERAEWRRRGVVGVVSALNPETK
                     EITLQTRGPQGPQPVTIAAAGEAVKFRRYAPDSVKFADAKPSAFQDLKVGDQLRAKGE
                     KSADGARFTPEEIVSGAFRTVVGNVTAVNAEKNEVSITQMQGGAPLTVVVSRDSVLKQ
                     VPAEFAARFGGGPGGPGGDGPGGAPGAGGQGQQPAGGGQAAQGGGQGAAPRPAGGGPS
                     GGGGEQAG"
     gene            complement(1555..>2600)
                     /locus_tag="AC159P_13480"
     CDS             complement(1555..>2600)
                     /locus_tag="AC159P_13480"
                     /inference="COORDINATES: protein motif:HMM:NF024853.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HIR3696505.1"
                     /translation="FLREQRQRDLAAKLADRLRATAAVKTNVAKATPPASPADRARVF
                     ATVNGRNITSGDIEDSLRPLVFSVQEQVYNLRKGSLDLKINDILLTAEAQKRTVTTRA
                     LIEAEVKPAAITDAEAQKFYDENKERINGDFAQVKPQIVEYLQQQARQKAESDFAKRL
                     RAAATVQTFLTEPVPPTYDIATDDQPAKGAATAPVTLVEFTDYQCPSCAQQFPVIERI
                     IAEMGDRVRVVVRDYPLTMHANAFKAAEAAEAAREQGKYWEYTALLFRNQSALEVGNL
                     KQYATSLGLDRARFDAALDSGKFSDKVRRDILDGDKVGVTGTPSLFVNGRRVNDRTYE
                     GLKAAIEAALKKK"
BASE COUNT          373 a          836 c          906 g          485 t
ORIGIN      
        1 gccggcctgt tcgccgccgc cgccactcgg cccgccgcca gcgggtcgcg gcgctgcacc
       61 ctgaccaccg ccttgagcgg cttgaccgcc gcccgcgggt tgctgacctt gaccgcccgc
      121 gcccggagcg ccgccggggc catcaccgcc cggcccgccc ggcccgccgc cgaaacgcgc
      181 cgcgaactcg gcgggaacct gtttgagcac agagtcgcgg ctgacgacca cggtgagcgg
      241 ggcgccgccc tgcatctgcg tgatggagac ttcgttcttc tccgcgttga cggcggtgac
      301 gttgccgacc acggtgcgga aagcgccgct gacgatctcc tcgggggtga agcgcgcgcc
      361 gtcggcgctc ttctcgccct tggcgcggag ctggtcgccg accttcaaat cctgaaacgc
      421 ggagggcttg gcgtccgcga acttcacgga gtcgggcgcg tagcggcgga acttgaccgc
      481 ctcgccggcg gcggcgatgg tgacgggctg cggcccctgc gggccgcgcg tctgcaaggt
      541 gatctctttg gtctcggggt tcagcgccga gacgacgccg acgacgccgc ggcggcgcca
      601 ctcggcgcgc tcggcctcgc gcttctgctc gatgtcgccc ttcgacatca cgatgacctg
      661 gcgcggcatc accgccttgc ggtcgtccga gaccttgccg cgcgcgaaga cgcggtcgcc
      721 cgtcgcgatg tccttgagcg cgatgtcggt ggccttgagg agcgaggtct cgccgggcgg
      781 caccttcttg tacgagacgc cttcggcgag cgtgacgatg acgaccgagc cggcgtccgt
      841 cttgacgaaa atctgtcggg cgttgacatc gaccgcgacc acctcgccga gcacgccgtt
      901 ggcgttgatg gcggggtcgc cgccttgcgc cggctgctgc ggcggcgcgg gcgtctgcgc
      961 gaaagcgcgc gcaccacccg cgaggacggc agccgacagg gcgataacgg ctatctgttt
     1021 tctcatgcta tgaaccctcg aatgataaaa ggagaacgcc gcgtgcgcgg gcatctacaa
     1081 actcggttcg gtatgattga ttcgcccctg agactgccga gcgacaaatc ttatacgacc
     1141 gagcgagacg atagttccca cgcgttggcg caaaaagcgc acccactttc gccccgacaa
     1201 acgcacataa gtgatgacgg ttctcgggca gaacccgtta accatccttc atattctgcc
     1261 gggatgcggg gaaagttcca cactttgcgg gggtgcgcgg actcgacacg ttttagacgc
     1321 gcctgcgcgc gtcgaggttg gtacatctcg ccgtcgaaaa tctatttata tggatgggaa
     1381 gaagtaggca gtaggcggta ggcggcaggc agtcggagca gacgcaatga gagccatggg
     1441 actttacctc ccaagcctct tcccctgtcg cttgatgaac gaagaggctt gggacgctac
     1501 gtctcaagcc tcttgctctt tgctcttctg tttttactgc ctactgccta ctgcctactt
     1561 cttcttgagc gcggcctcga tggcggcctt cagaccttcg taggtgcggt cgttgacgcg
     1621 cctgccgttg acgaagagcg agggcgtgcc ggtaacgccg accttgtcgc cgtcgaggat
     1681 gtcgcgccgc accttgtcgg agaacttgcc ggagtcgagc gcggcgtcga agcgcgcgcg
     1741 gtcgaggccg aggctcgtcg cgtactgctt gaggttaccg acttcgagcg ccgactggtt
     1801 gcggaacagc agcgccgtgt actcccagta cttcccttgc tcgcgcgcgg cctcggcggc
     1861 ctcggcggcc ttgaaggcgt tggcgtgcat cgtgagcggg tagtcgcgca cgacgacgcg
     1921 gacgcggtcg cccatctcgg cgatgatgcg ttcgatgacg gggaactgct gcgcgcagct
     1981 cgggcactgg tagtcggtga actcgacgag ggtgacgggg gccgtggccg cgcccttcgc
     2041 gggctggtcg tcggtggcga tgtcgtaggt gggcggcacc ggctccgtca ggaaggtctg
     2101 aacggtcgcg gcggcgcgca gccgcttggc gaagtccgat tcggccttct gccgcgcctg
     2161 ctgctggagg tactcgacga tctgcggctt gacctgcgcg aagtcgccgt tgatgcgctc
     2221 cttgttctcg tcgtagaact tctgcgcctc ggcgtcggtg atcgccgccg gcttgacctc
     2281 tgcttcgatc agcgcgcggg tcgtgaccgt gcgcttctgc gcctcggctg tgagcaggat
     2341 gtcgttgatc ttcaagtcga gcgagccctt gcgcaggttg tagacctgct cctgcacgga
     2401 gaagacgagc gggcgcagcg agtcttcgat gtcgccggag gtgatgttcc tgccgttgac
     2461 ggtggcgaag acgcgggcgc ggtctgccgg gctggcgggc ggcgtggcct tcgcgacgtt
     2521 ggtcttgacg gcggcggtgg cgcgcaggcg gtcggcgagc ttcgccgcga ggtcgcgctg
     2581 ccgctgctcg cgcaggaagg
//
LOCUS       DBNGOH010000696         7054 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4502194, whole genome shotgun sequence.
ACCESSION   DBNGOH010000696 DBNGOH010000000
VERSION     DBNGOH010000696.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7054)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7054)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7054
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4502194"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(210..1628)
                     /locus_tag="AC159P_13485"
     CDS             complement(210..1628)
                     /locus_tag="AC159P_13485"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HIR3696506.1"
                     /translation="MTRTGIFHHSMTRTNIFRRRSIVILLSIVFFVAAEARAQPGAVN
                     FDELEKVVLDELRETKTPGAAIAIVNGGRVVYAKGFGVRDVETNTPVTADTLFRLGST
                     TKMFTGAAAVVLAEQGKIRLDAPVGDYAKGLPPKLSRVTAHQLLSNAAGVADFSPPSV
                     SHDDAVLAAMVRGWKDDALFGEPGKVYSYSSPGFWLAGYVIEEAGGKPYADMMEELLF
                     KQLGMARTTFRPHMAMTYPLALGHNTQDGKAAIIRPAFNNVAMWPAGSIFSSANDLAR
                     FVVALLNNGRLEDRQVLPASLFTKLSGEHVSMPGEPSVHYGYGLLNFKDRGARLVMHG
                     GFSRGYGSMIQMVPERGFAVIVVANRSGETLRRTTEKAKDLFLKLEPQPSQPRQAQPL
                     AEGDAAKFVGRYVNGPQTWEVMAKEGRLFLKDAGGEYELKKTGDLRLSYGDALEADAA
                     FVLGPDGKAEYIFTGLYAARRN"
     gene            complement(1866..2564)
                     /locus_tag="AC159P_13490"
     CDS             complement(1866..2564)
                     /locus_tag="AC159P_13490"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006926727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic pyrophosphatase"
                     /protein_id="HIR3696507.1"
                     /translation="MSDNDKALHELLSLMFQAHPWHGSTAGEEAPSVVNAYIEIVPTD
                     AVKYELSKRTGHLRLDRPQRYSSFPPTLYGFIPQTFCGDRVAALCEERGGGEGIRGDG
                     DPMDICVLTEKPLAHGGLFVRARPIGGLRMIDGNEADDKIVAVLDRDVAYGHIEDIGA
                     VPPGLVERLQHYFLTYKQLPQDAPRRVEIAEVYDRAEALNVIGRSFEDYRARFGAPED
                     RVGELRRLLKSTED"
     gene            2739..3827
                     /locus_tag="AC159P_13495"
     CDS             2739..3827
                     /locus_tag="AC159P_13495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696508.1"
                     /translation="MSATTTTTTAQAAGLAEERALSVRWIISARDDLVWFIGSVATSY
                     ALIALYVGGLVPLVPMVVAWAVLVDAPHVFATFSRTYFDREERRARRRLLWWSLLFFL
                     AGPVAVFAGAGFVFFFVAALWAYYHLVKQHYGFMVLYKKKNRDLAVVDNALDRAFLLL
                     AFTYPFVAFVARDREAMARVPSVLLGGIGYLEAALLAATVVVGAAWLGRQVQRALTGM
                     PLNVPKYLLLAAAIPMHWVVLLTPMPHKPIAIVAMLTVYHNLQYHRLVWFHNQKYKRD
                     EAARERHGAAEFISRRLVYYVVFGVLFGIWYQVPRQYVNHAADPASFTTQLASGFLWG
                     YAFIHYHLDAKIWRVRRDPSVGKALRME"
     gene            complement(3943..4236)
                     /locus_tag="AC159P_13500"
     CDS             complement(3943..4236)
                     /locus_tag="AC159P_13500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696509.1"
                     /translation="MAQTATAAATRADNDFTNAANGLDKDVVESTLRTFDQSTKSATG
                     VSLFHILTLGSIGASLALYFTGNKTAAIFVGLWPPTFQALKGVADTNKENRKQ"
     gene            complement(4259..4402)
                     /locus_tag="AC159P_13505"
     CDS             complement(4259..4402)
                     /locus_tag="AC159P_13505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696510.1"
                     /translation="MNDGMVAAGRGAMGEGRTRGLLIAAASLALLWALAFLKGRAKSD
                     LSN"
     gene            complement(4438..4893)
                     /locus_tag="AC159P_13510"
     CDS             complement(4438..4893)
                     /locus_tag="AC159P_13510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR3696511.1"
                     /translation="MRFVKESIIRAAPERVFAFHELPDALARLTPPWEHARVVRAAPD
                     LRVGAEAVVETRVFGLVPLKWVARHTAYDPPRMFEDVQVEGPFRRWRHRHVVAPHAEG
                     ATLRDEIEYEPPFGFLGRLAAPALVVGRLRKLFDYRHEVTRAWCEDGAD"
     gene            5094..5978
                     /locus_tag="AC159P_13515"
     CDS             5094..5978
                     /locus_tag="AC159P_13515"
                     /inference="COORDINATES: protein motif:HMM:NF014375.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing protein"
                     /protein_id="HIR3696512.1"
                     /translation="MSMTFTEFMTTRQLARLWKVSEATIKRWADAGHLRPSRTLGGHR
                     RFSLEEVARFQSERGLTQTAATKTALGRVGGAAAPAWPTGGPVGEVTEAFYESLAAGR
                     EATATAVLLEAYLAGASVAELLDEVVAGALRRVGDEWEAGRATVADEHLATRTATRAV
                     ETLRGAVKREGAARGLAVCCAAEEELHELAVLCAQTLLESEGWEVRNLGGNTPFFALC
                     DAVERHRPRLVCVSSTTRGALAGAARDYAQCLAAARAGGARVVLGGAGFRGEGVREKF
                     PADLHAESLSALLEFTRD"
     gene            6175..6756
                     /locus_tag="AC159P_13520"
     CDS             6175..6756
                     /locus_tag="AC159P_13520"
                     /EC_number="1.13.11.20"
                     /inference="COORDINATES: protein motif:HMM:NF017778.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0017172 - cysteine dioxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine dioxygenase"
                     /protein_id="HIR3696513.1"
                     /translation="MSTANTQSSSAPPPAQAEDRPACRTLAALADRLISLTAEPSLAE
                     INAWVREVRVERAELEPFVQFKDTCYSRRRVVRGEHAELLIICWRPGQRTPIHDHDGS
                     YGTVRVCEGVMWETIFGMEDARGLAYRMGREWRAGETTAGADIPDIHQIGNPDISGQN
                     LITLHLYAPPLTSLNIYRVGHTDSEFTPSVEIE"
     gene            complement(6857..>7054)
                     /locus_tag="AC159P_13525"
     CDS             complement(6857..>7054)
                     /locus_tag="AC159P_13525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein R"
                     /protein_id="HIR3696514.1"
                     /translation="ADGDYNNNRELYLKHQYEGAELDINYARRVLEHVHTLWGRPVHL
                     ETTVDDERVRLHYDGEEHDED"
BASE COUNT         1104 a         2413 c         2285 g         1252 t
ORIGIN      
        1 gggcgaggcc gaagagcgcg agccgcgcct ctcttcgacg acgctcgcgc gctggaagga
       61 cgccgcacgc cgcacgctcg tctgcccgcg ctgcggcgcc gaagaagaaa tgatgaatgc
      121 ggaatgatga atgatgaata agaagcacgc tgcacgtcat cactcgactt gtctttaatt
      181 catcattctg cattctgcat tcatcattat taattcctcc tcgccgcata cagccccgtg
      241 aagatgtact cggccttgcc gtcggggccg aggacgaagg ccgcgtccgc ttcgagcgcg
      301 tcgccgtagg agagccgcag gtcgcccgtc ttcttcagct cgtactcgcc gcccgcgtcc
      361 ttcaaaaaga gccgcccctc cttcgccatc acctcccacg tctgcggccc gttgacgtaa
      421 cgtccgacga acttcgccgc gtcgccttcg gccagcggct gcgcctgccg cggctgcgag
      481 ggctgcggtt cgagcttgag aaacagatcc ttcgccttct cggtcgtgcg ccgcagcgtc
      541 tcgccgctgc ggttggcgac gacgatgacg gcgaagccgc gctccggcac catctggatc
      601 atcgagccgt agccgcggct gaagccgccg tgcatgacga gccgcgcgcc gcggtctttg
      661 aagttgagca gcccgtagcc gtaatgcacc gaaggctcgc ccggcatcga gacgtgctcg
      721 cccgagagct tcgtgaagag cgacgcgggc aagacctgcc ggtcttcgag ccgcccgttg
      781 ttgaggagcg cgacgacgaa gcgcgccaga tcgttcgccg aagagaagat cgagccggcg
      841 ggccacatcg ccacgttgtt gaaggccggg cggatgatgg ccgccttgcc gtcctgcgtg
      901 ttgtgcccga gcgcgagcgg gtaggtcatc gccatgtgcg ggcgaaaggt cgtgcgcgcc
      961 atgccgagct gcttgaacag cagctcctcc atcatgtccg cgtagggctt gccgcccgcc
     1021 tcttcgatga cgtagcccgc cagccagaag ccggggctgg agtacgagta gaccttgccc
     1081 ggctcgccga agagcgcgtc gtccttccac ccgcgcacca tcgccgcgag caccgcgtcg
     1141 tcgtgcgaga cggacggcgg cgagaagtcc gcgacgcccg ccgcgttgct cagcagttgg
     1201 tgcgcggtga cgcgcgagag cttcggcggc agccccttcg cgtagtcgcc caccggcgcg
     1261 tcgagtctga tcttgccctg ctcggcgagc acgaccgccg ccgcgcccgt gaacatcttc
     1321 gtcgtcgagc cgagccggaa gagcgtgtcc gccgtgaccg gcgtgttcgt ctccacgtcg
     1381 cgcacgccga agcccttcgc gtagaccaca cgcccgccgt tcacgatggc gatggccgcg
     1441 cccggcgtct tcgtctcgcg cagctcgtca agaacgacct tctccaattc atcgaagtta
     1501 accgcacccg gctgcgcgcg cgcctcggcg gcgacgaaga aaacgattga cagaagaatg
     1561 acgatcgagc ggcggcggaa gatgttcgtt cgagtcatcg agtggtggaa gattcctgtt
     1621 cgagtcatgg tgagttcgcg tgtgccctgc gacgcgccgg gttcgcgcga cggcgggctt
     1681 cataagttaa accgtttcgc ttctcggcgg gaaactttgt gacgaccggg ccgcgccgcg
     1741 ggacgagcgg gagaatcaag tgatgaggac tgagtacgag ccgtgaaccg tcaaccgtca
     1801 accgtgacag gtgaagacct gttccgactc gtcacggttg acggtttacc cgtcacggct
     1861 tttactcagt cctcggtact tttcagcagc cggcgcagtt cgccgacgcg gtcttcgggc
     1921 gcgccgaagc gtgcgcgata gtcttcgaag ctgcgcccga tgacgttgag cgcttcggcg
     1981 cggtcgtaga cttcggcgat ctcgacgcgg cgcggcgcgt cctgcggcag ttgcttgtag
     2041 gtgaggaagt agtgttgcag ccgctcgacg aggcccggcg gcaccgcgcc gatgtcttcg
     2101 atgtggccgt aggccacgtc gcggtcgagg acggcgacga tcttgtcgtc ggcctcgttg
     2161 ccgtcgatca tgcgcaggcc gccgatgggc cgcgcgcgga cgaagaggcc gccgtgcgcg
     2221 agcggctttt cggtcaggac gcagatgtcc atcgggtcgc cgtcgccgcg gatgccttcg
     2281 ccgccgccgc gctcctcgca gagcgcggcc acgcggtcgc cgcagaaggt ctgcgggatg
     2341 aagccgtaga gcgtcggcgg gaacgaagag tagcgctgcg ggcggtcaag ccgcaggtgg
     2401 cccgtgcgct tgctgagttc gtacttgacg gcgtccgtgg gcacgatctc gatgtaggcg
     2461 ttgaccaccg agggcgcctc ctcgcccgcc gtcgagccgt gccacgggtg tgcctgaaac
     2521 atgagcgaga gcagttcgtg cagcgccttg tcgttgtctg acatctcggc tgagatcctc
     2581 cgctcgcgac ccggcggcgc ggcgcgggcc ggcccgcccc cttgtgataa aatcgccccg
     2641 cctcgccggt ctgaattttc tggattatga gcgccgcgcc gttcggtgtc caacacgcgc
     2701 gcggcgtgcg cgaggctctt tcagccgctt cagctttcat gtccgccacg acgacaacga
     2761 cgacggcgca ggccgcaggg ctggccgaag agcgcgccct ctccgtgcgc tggatcattt
     2821 cggcgcgcga cgacctcgtg tggttcatcg ggtcggtggc gacgagctac gcgctgatcg
     2881 cgctgtacgt cggcgggctg gtgccgctcg tgccgatggt ggtcgcgtgg gcggtgctcg
     2941 tggacgcgcc gcacgtcttc gccaccttct cgcgcaccta cttcgaccgc gaggagcggc
     3001 gcgcgcgccg caggctcctc tggtggtcgc tcctcttctt cctcgccggg ccggtcgcgg
     3061 tcttcgcggg cgcggggttc gtcttcttct tcgtcgcggc gctgtgggcc tactaccacc
     3121 tcgtcaagca gcactacggc ttcatggtgc tctacaagaa gaagaaccga gacctcgccg
     3181 tcgtcgataa cgcgctcgac cgcgcgttcc tgctgctggc gttcacgtat cctttcgtcg
     3241 ccttcgtcgc gcgcgaccgc gaggcgatgg cgcgcgtccc ctcggtcttg ctcggcggca
     3301 tcggctatct ggaagccgcg ctgctcgccg cgaccgtggt ggtgggcgcc gcgtggctcg
     3361 gcaggcaggt gcagagggct ttgacgggga tgccgctgaa cgtgccgaag tacctgctct
     3421 tggcggcggc gatcccgatg cactgggtcg tgctgctgac gccgatgccg cacaagccca
     3481 tcgccatcgt cgccatgctg accgtctatc acaacctcca gtaccaccgg ctcgtctggt
     3541 tccacaacca gaagtacaaa agggacgagg ccgcgcgcga acgccacggc gcggcggagt
     3601 tcatcagccg caggctcgtc tattatgtcg tcttcggcgt cctcttcggc atctggtatc
     3661 aggtgccgcg ccagtacgtg aaccacgccg cagaccccgc ctccttcacc acccagctcg
     3721 cctccggctt cctctggggc tacgctttca tccactacca cctcgacgcg aaaatctggc
     3781 gcgtgcgccg cgacccttcg gtcggcaagg cgctgcggat ggaataaggc ggcaggggaa
     3841 gcgttacaag gtaaggggcg aaacggagaa gaaaatcctc ttccgtttcg cccattccct
     3901 tgtaacgctt cccccttgac tccccttagg ctcgcgcgcg cgttactgct tccgattttc
     3961 cttgttcgtg tcggccacgc ccttgagtgc ctggaaggtg ggcggccaga ggccgacgaa
     4021 gatggccgcc gtcttgttgc ccgtgaagta gagcgccagc gaggcgccga tggagccgag
     4081 cgtcaggatg tggaacaggc tgacgccggt tgcgctcttg gtcgattggt caaacgtgcg
     4141 gagcgtggac tcgaccacgt ctttgtcgag accgttcgcc gcgttggtga aatcgttgtc
     4201 ggcgcgcgtc gccgccgcag tcgctgtctg tgccattgtc tgtcttctcc cgtgttcgtt
     4261 aattgctcaa atccgacttc gcgcgccctt tcagaaacgc caaagcccat agcagcgcga
     4321 gcgaggctgc cgcgatcagc agcccgcgcg tccgtccctc acccattgcc ccgcggcccg
     4381 ctgcaaccat cccgtcgttc atcacccgcc cctttctcgt tctccccgcc ttgaaactta
     4441 atcagcgcca tcctcgcacc acgcgcgcgt cacctcgtgg cggtagtcga aaagttttcg
     4501 cagccgcccg acgacgagcg cgggcgccgc gagccgaccc agaaaaccga acggcggctc
     4561 gtactcgatc tcgtcgcgca aggtcgcgcc ctcggcgtgc ggcgcgacga cgtgcctgtg
     4621 acgccagcgg cggaaaggcc cttcgacctg cacgtcctcg aacatccgcg gcgggtcgta
     4681 ggccgtgtgc cgcgcgaccc acttcagcgg caccagcccg aacacgcgcg tctcgaccac
     4741 ggcctccgcg ccgacgcgta agtccggcgc ggcccgcacc acgcgcgcgt gttcccacgg
     4801 cggcgtcagg cgcgcgaggg cgtccggcaa ttcgtggaac gcgaacacgc gctcgggcgc
     4861 ggcgcgaatg atgctctcct tgacgaatct catggtcgag cattctatcc cgaggcgtcg
     4921 tcaatcgttt tcggcgcttc aaatatcttc aaaaatcatc atacaccaat cgctcgaacc
     4981 gctcatcacg accgaattcc gaacctcata cgtttctttg tcggtcgctt tccgccgaga
     5041 aactgcttga cacgtgttgc cgagggctta tgctgtgagc gcttgaaatg ctcatgagca
     5101 tgaccttcac ggaattcatg acgacccgcc aactcgcgcg cctgtggaag gtgagcgagg
     5161 cgacgatcaa gcgctgggcc gacgccgggc acctgcgccc aagccgcacg ctcggcggcc
     5221 accggcgctt ctcgctcgaa gaggtggcgc ggttccagag cgagcgcggg ctgacgcaga
     5281 ccgcggcgac gaagaccgcg ctcggcagag tcggcggggc ggcggcgccc gcgtggccca
     5341 cgggcggccc ggtgggcgag gtcacggaag ccttctacga atcgctcgcc gccgggcgcg
     5401 aggcgacggc cacagccgtc ctgctcgaag cgtatctggc gggcgcgagc gtggcggagc
     5461 tgctggacga agtggtcgcg ggcgcgctgc gccgcgtcgg cgacgagtgg gaggcaggtc
     5521 gcgccaccgt cgccgacgag cacctcgcca cgcgcacggc gacgcgcgcc gtcgagacgc
     5581 tgcgcggcgc cgtcaagcgc gaaggcgccg cgcgcggact cgccgtctgc tgcgccgccg
     5641 aggaggagct gcacgagctg gccgtgctct gcgcgcagac cttgctcgaa agcgaagggt
     5701 gggaggtgcg gaacctcgga ggcaatacgc ccttcttcgc gctctgcgac gccgtcgagc
     5761 gccaccgccc gcgcctcgtc tgcgtctctt cgaccacgcg cggcgcgctt gccggggcgg
     5821 cgcgcgacta cgcgcaatgc ctcgccgcgg cccgcgccgg gggcgcgcgc gtcgtgctcg
     5881 gcggcgcggg cttccgcggc gaaggcgtgc gcgagaagtt ccccgcagac ctccacgccg
     5941 agagcctctc cgcgcttctg gagttcacgc gcgactgatt tcggatatcg gatttcggat
     6001 tggcgtgcga ctcggacttg atgcctgttg acggaagtcc aaccttcgcc gcccggtgaa
     6061 tccaaaatcc gatatccgaa atccaaaatt ccgcgtttga cttccccacc gttaggcgtt
     6121 agaaaagttc acgtcctgca gcagccatcg cctttttccg aggagtcgct tttcatgtcc
     6181 accgccaaca cacagtcgtc gtccgccccg ccgcccgcac aggcggaaga ccggcccgcg
     6241 tgtcgcacgc tcgccgcgct cgccgacagg ctcatctctt taaccgccga gccgtccctc
     6301 gccgaaatca acgcgtgggt gcgcgaggtg cgcgtcgagc gcgcggagct ggagccgttc
     6361 gtccagttca aggacacgtg ctactcgcgc cgccgcgtcg tccgcggcga gcacgcagag
     6421 ctgctcatca tctgctggcg ccccggacag cgcacgccca tccacgacca cgacggctcc
     6481 tacggcaccg tgcgcgtctg cgagggcgtc atgtgggaga ccatcttcgg gatggaggac
     6541 gcgcgcggcc tcgcctaccg gatggggcgc gagtggcgcg cgggcgagac gaccgccggc
     6601 gccgacatcc ccgacatcca ccagatcggc aaccccgaca tcagcggcca gaacctcatc
     6661 acgctccacc tctacgcgcc cccgctcacc agcctcaaca tctaccgcgt cggccacacc
     6721 gactccgaat tcaccccctc ggtcgaaatc gaatagaaga aggggaaaag gggaaaaggg
     6781 gaagtgggga agtggaagaa gcacgtcgct ggtcttccct tttcccctct tccccttttc
     6841 ccctttcccc cggctcttaa tcctcgtcgt gctcctcgcc gtcgtagtgg aggcggacgc
     6901 gctcgtcgtc cacggtggtc tccaggtgga cggggcggcc ccagagggtg tggacgtgtt
     6961 cgaggacgcg gcgggcatag ttgatgtcca gctccgcccc ttcgtactgg tgcttgagat
     7021 acagctcgcg gttgttgttg tagtcaccgt cggc
//
LOCUS       DBNGOH010000697         2418 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3769270, whole genome shotgun sequence.
ACCESSION   DBNGOH010000697 DBNGOH010000000
VERSION     DBNGOH010000697.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2418)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2418)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2418
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3769270"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1971)
                     /locus_tag="AC159P_13530"
     CDS             complement(<1..1971)
                     /locus_tag="AC159P_13530"
                     /inference="COORDINATES: protein motif:HMM:NF012311.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HIR3696515.1"
                     /translation="MAQLPTTARRFVALALALLLLHLPASAGVVVYRGTQGLTLTGTD
                     GVYYDNTQGLTMTGTDALLGLSVNGIGFGATGDGLTMTGTDTQSVAGADGVTYTGANS
                     YTAAHADGLTMTGTDSLNMTSADGLTMTGTDGTVYEADSVIIRQADGLTMTGTDALTL
                     TGVNGLTMTGTDTISFVWADGITAAGAEGVRLDGAAEVVGVRSDGTFFQAPTDGLVFS
                     RVDGLTMTGTDAVWMYGVEGLTMTGTDAWAMTGTDQQPQQGMVGFDPELALALNRLTL
                     ERLTDDSNVNAAFVYHRAVTDADIEELQRIGIRGGTRFRVLPVVLATGTANQIAAVSR
                     LPAVRSIYGNRTLRFDADTSRALAGLARARADADLARTGGAALDGTGVGVAVIDTGLD
                     ATHPDLAGRVAQNVKLADLQGASPLGFAPPANVENLPNTDLIGGHGTFVGGLVAGSGA
                     ASAGRYAGYAPGARLVGLGAGDLSLFHVLAGFDYLLARPDLGVRVVNCSFSANTLYSE
                     HDPVNVATRMLAERGVNVVFSAGNGGPGLRTLNPYAAAPWVISVGATDARGRLADFSA
                     RGDFGSRTFRPTLVAGGVGVVSLRAGGANQTGAGEAATGDAQLTADELPYYTTASGTS
                     FSAPQVAGTIALMLQANPQLTPAEVRDILQRTA"
     gene            complement(2247..>2418)
                     /locus_tag="AC159P_13535"
     CDS             complement(2247..>2418)
                     /locus_tag="AC159P_13535"
                     /inference="COORDINATES: protein motif:HMM:NF015649.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bacterial transcriptional activator
                     domain-containing protein"
                     /protein_id="HIR3696516.1"
                     /translation="DPFREDAHCMVMRAHAALGNRVAVREQYETLKKLLRKELGVEPA
                     ADTQKAYRQMLG"
BASE COUNT          332 a          828 c          877 g          381 t
ORIGIN      
        1 cgccgtccgc tggaggatgt cgcgcacttc ggcgggcgtg agttgcgggt tcgcctgaag
       61 catgagggcg atggtgcccg ccacctgcgg cgcgctgaag gaggtgccgg aggccgtcgt
      121 gtagtaaggc agctcgtccg ccgtgagctg cgcgtcgccc gtggcggctt cgcccgcccc
      181 tgtctggttc gcgccgcccg cgcgcaggct gaccaccccg acgccgcccg cgaccagtgt
      241 cgggcggaag gtgcggctgc cgaagtcgcc gcgcgcgcta aagtccgcga gccgcccgcg
      301 cgcgtccgtc gcgcccacgg agatgaccca cggcgccgcc gcgtaagggt tgagcgtgcg
      361 caggcccggc cccccgttgc ccgccgagaa gacgacgttg acgccgcgct cggccagcat
      421 ccgcgtggcc acgttcacgg ggtcgtgctc gctgtagagg gtgttcgcag agaagctaca
      481 gttgacgacg cgcacgccga ggtctgggcg cgcgagcaga tagtcgaagc ccgcgaggac
      541 gtggaagagc gagaggtcgc ccgcgccgag gccgacgagc cgcgcgcccg gcgcgtagcc
      601 cgcgtacctg cccgccgacg cggccccgct gcccgcgacg aggccgccga cgaacgtgcc
      661 gtgccctccg atcaaatccg tgttcggcag attctcgacg ttggccggcg gcgcgaagcc
      721 taaggggctg gcgccctgca agtccgcgag cttgacgttc tgcgcgacgc ggcccgcgag
      781 gtccgggtgc gtcgcgtcga ggccggtgtc gatgacggcg acgccgacgc ccgtgccgtc
      841 gagcgccgcg ccgcccgtgc gcgcgaggtc cgcgtccgcg cgtgcgcggg cgaggcccgc
      901 gagggcgcgg ctcgtgtccg cgtcgaagcg gagcgtccgg ttgccgtaga tggagcgcac
      961 cgcgggcagt ctggagacgg cggcgatctg gttggcggtg cccgtcgcca agaccacggg
     1021 cagcacgcgg aagcgcgtcc cgccgcggat gccgatgcgc tggagttcct cgatgtcggc
     1081 gtcggtgacg gcgcggtgat agacgaaggc cgcgttgacg ttgctgtcgt cggtcaggcg
     1141 ttcgagcgtc aaccggttga gcgcgagcgc gagttccggg tcgaagccga ccatcccctg
     1201 ctgcggctgc tggtcggtgc cggtcatcgc ccaagcgtcg gtgccggtca tcgtcagccc
     1261 ctcgacgccg tacatccaga cggcgtcggt gccggtcatc gtcaggccgt caacccgcga
     1321 gaagacgagg ccgtccgtcg gcgcctggaa gaaggtgccg tccgagcgga cgccgacaac
     1381 ctcggcggcg ccgtcgagcc gcacgccctc ggcgcccgcc gccgtgatgc cgtcggccca
     1441 gacgaacgag atcgtgtccg tgcccgtcat cgtgaggccg ttcacgcccg tcaacgtcag
     1501 cgcgtcggtg cccgtcatcg tcagcccgtc ggcctgccgg atgatgaccg agtcggcctc
     1561 gtagacagtc ccgtccgtgc cggtcatcgt gaggccgtcg gcgctcgtca tgttgaggct
     1621 gtcggtgccc gtcatcgtca ggccgtcggc gtgcgccgcc gtgtagctgt tggccccggt
     1681 gtaggtcacg ccgtcggcgc ccgccacgct ctgcgtgtcg gtgccggtca tcgtcaggcc
     1741 gtcgcccgtc gccccgaagc cgatgccgtt gaccgagagg ccgagcagcg cgtccgtgcc
     1801 cgtcatcgtc agcccctgtg tgttgtcgta gtagacgccg tcggtgcccg tcaacgtcag
     1861 cccctgcgtg ccgcggtaga cgacgacgcc cgcgctcgcc ggcaggtgca agagcaggag
     1921 cgcgagcgcg agcgcgacga aacgtctggc cgtagtcggg agttgagcca tcgtcgattg
     1981 cctgacgggc gaaaggggta aagcgattcg aagtccgggg aaaaacaggc tccgggcggc
     2041 aggcataacc cgcgtcggga gcaggctaca ggcgaaggcc gggggtctcg atttcaagcg
     2101 acgcacttat aacagttcct ctatcagcta ttgttacggt tgtgtcccat ttttcttata
     2161 gctctattgc tacaaattcg tgcgtttttc gcggcaccac agggcgagcg ccggtcggct
     2221 gcgcgccgca cgctcgttcg aaacggctag ccgagcatct ggcggtaagc cttctgcgtg
     2281 tccgccgccg gctcgacgcc gagttccttc cggaggagct tcttcagcgt ctcgtactgc
     2341 tcgcgcacgg cgacgcggtt gccgagcgcg gcgtgcgcgc gcatgaccat gcagtgcgcg
     2401 tcctcgcgga aagggtcg
//
LOCUS       DBNGOH010000698         2147 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_287177, whole genome shotgun sequence.
ACCESSION   DBNGOH010000698 DBNGOH010000000
VERSION     DBNGOH010000698.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2147)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2147)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2147
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_287177"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1827)
                     /gene="asnB"
                     /locus_tag="AC159P_13540"
     CDS             complement(<1..1827)
                     /gene="asnB"
                     /locus_tag="AC159P_13540"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0070981 - L-asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HIR3696517.1"
                     /translation="MCGIAGIVNREDRPVDAALLARMNEAIRHRGPDEDGTYLNGRVG
                     MAMRRLAIIDLKSGQQPITNEDRTIWIVYNGEIYNFRELKDRLEKLGHTFKTDCDTEA
                     VVHAYEQYGTDCPKYFRGMFAFAIWDERKQELFLARDRVGKKPLLYSYANGSFVFGSE
                     FSALLAHPSVGREVDREAIHNYLTFMCVPAPLTAYRDIRKLEPGHSLRFTRDGEIKTE
                     RYWQPDFSKKAKLTEEEAGERVLEILRDAVRVRLMSEVPLGAFLSGGVDSSTVVALMS
                     EVSSAPVKTFSIGFEEQDFSELHHARRVAEHVGAEHHEFVVRPDAMEVLPTLVEHYGE
                     PYADSSAIPTYYVSRETRRHVTVALNGDGGDECFAGYERYAAMRLSERYRKLPGLLRD
                     NVIEQAVGLLPSSELKRSRVRSLKRFLRAASLPPVERYLRWVSVLDARAKTELYTDDF
                     LRETADDDPARWLAPWFARANGAGVVDAALLADTMTYLPNDLLVKVDIASMAVSLEAR
                     SPVLDHHVIEFAASLPEGLKLRGLTTKYLLKKTLGRLLPSENLKRAKMGFGVPVGHWF
                     RGEMQGFLRENLLSEKSSRRGYLKPEAVRRMVEEHTAGRRDHT"
     gene            complement(1830..>2147)
                     /locus_tag="AC159P_13545"
     CDS             complement(1830..>2147)
                     /locus_tag="AC159P_13545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696518.1"
                     /translation="RQALPIVLFTVMLTLAYSIFQGNIGTAYRQRSQILVFYFIFVAV
                     GAVLLKEQQEDRREQAIAERRAIVEETARKNESRKRFQSWKRNQEKELEEIARGISER
                     IDF"
BASE COUNT          338 a          682 c          740 g          387 t
ORIGIN      
        1 cgtgtggtcg cgccgccccg cggtgtgctc ctcgaccatg cggcgcaccg cttcgggctt
       61 gaggtagccg cgccgcgaag acttttccga gagcaggttc tcgcgcagga acccctgcat
      121 ctcgccgcgg aaccagtggc cgacgggcac gccgaagccc atcttggcgc gcttcaggtt
      181 ctccgacggc aacagccgcc cgagcgtctt cttcaagagg tacttcgtcg tcagcccgcg
      241 cagcttcaag ccctcgggca ggctggccgc gaactcgatg acgtgatgat cgaggaccgg
      301 cgagcgcgct tccagagaga cggccatgct ggcgatgtcc actttgacga gcaggtcgtt
      361 cggcaggtag gtcatcgtgt cggcgaggag cgcggcgtcc acgacgcccg cgccgttcgc
      421 gcgcgcgaac cacggcgcga gccagcgcgc ggggtcgtcg tccgccgtct cgcgcaggaa
      481 gtcgtccgtg tacagctcgg tcttggcgcg cgcatcgagc acgctcaccc agcgcaggta
      541 gcgctcgacg ggcggcaacg aagccgcacg caggaagcgc ttgagcgagc gcacgcggct
      601 gcgcttcagc tcggacgagg ggagcagccc gaccgcctgc tcgatgacgt tgtcgcgcag
      661 caggccgggc aatttgcggt agcgctcgga caggcgcatc gcggcgtatc gttcgtagcc
      721 cgcgaagcat tcgtcgccgc cgtcgccgtt cagggcgacg gtgacgtggc ggcgcgtctc
      781 gcgcgagacg tagtaggtcg ggatggccga agagtccgcg taaggctcgc cgtagtgctc
      841 gaccagcgtc gggagcacct ccatcgcgtc cggtcggacg acgaactcgt ggtgctctgc
      901 gccgacgtgt tcggcgacgc ggcgggcgtg gtggagttcg gaaaaatcct gctcctcgaa
      961 gccgatggag aaggtcttga cgggggccga gctgacctcg ctcatcaagg cgacgacggt
     1021 gctcgaatcg acgccgcccg aaagaaacgc gccgagcggc acctcggaca tcagccgcac
     1081 gcgcacggcg tcgcgcagta tctccagcac gcgctcgccc gcttcctctt ccgtcagctt
     1141 cgccttcttc gagaagtcgg gctgccagta gcgctcggtc ttgatctcgc cgtcgcgcgt
     1201 gaagcgcaga gagtggcccg gctcaagctt gcggatgtcg cggtaggccg tgagcggcgc
     1261 gggcacgcac atgaaggtca gatagttatg gatggcctcg cggtcaacct cgcgcccgac
     1321 cgaggggtgc gcgaggagcg ccgagaactc ggagccgaag acgaacgagc cgttcgcgta
     1381 ggagtagagg agcggttttt tgccgacgcg gtcgcgcgcg aggaacagct cctgcttgcg
     1441 ctcgtcccag atggcgaagg cgaacatgcc gcgaaaatat ttcgggcagt ccgtgccgta
     1501 ctgttcgtag gcgtggacga ccgcctcggt gtcgcagtcg gtcttgaagg tgtggcccag
     1561 tttttcgagg cggtctttga gttcgcggaa gttgtagatt tcgccgttgt agacgatcca
     1621 gatcgtgcgg tcttcgttgg tgatgggctg ctggccgctc ttgaggtcga tgatggcgag
     1681 gcggcgcatg gccatgccga cgcgaccgtt cagatacgtg ccgtcctcgt ccggcccgcg
     1741 gtggcggatg gcttcgttca tccgcgccag gagcgcggcg tccactgggc ggtcttcgcg
     1801 gttgacgatc ccggcgatcc cacacatcgc tagaagtcta tcctctccga gatgccgcgc
     1861 gcgatctctt ccagttcctt ctcctggttc cgcttccagc tctggaaacg cttgcgggat
     1921 tcgttcttgc gcgcggtctc ttccacgatg gcgcgccgct ccgcgatggc ctgctcgcgc
     1981 ctgtcctcct gctgctcttt gagcagcacg gcgccgacgg cgacgaagat gaagtagaag
     2041 acgagtatct ggctgcgctg ccggtaggcc gtcccgatgt tgccctgaaa gatggagtag
     2101 gccagcgtca gcatcaccgt gaagagcacg atgggcagag cctgcct
//
LOCUS       DBNGOH010000699         4322 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_5052977, whole genome shotgun sequence.
ACCESSION   DBNGOH010000699 DBNGOH010000000
VERSION     DBNGOH010000699.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4322)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4322)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4322
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_5052977"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1275)
                     /locus_tag="AC159P_13550"
     CDS             complement(<1..1275)
                     /locus_tag="AC159P_13550"
                     /EC_number="2.7.7.-"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HIR3696519.1"
                     /translation="MAAVSLHLRRLRLRAGKARVDAESILKAEFEREALGAERLRVLV
                     LTIVITVSLLLSLIPPTVFSEGIEHVFRGEMQAFIFWRFLILFILALYVFSERILLGY
                     VFKNGRKVPGFYRYVTAFVETSFPTAEIILLASYTDDLVAALALPVFIYPVFIILSAL
                     RLNFRLCVFTGAVAALEYALVGMFYLDRAEGSTLNPLLVSAPLHAVKGVILLALGVVT
                     GLVALQIKKRILDSFKLAEERNDIVNTFGQHVSPAVVDKLLERGHDVRSEKRDVCVMF
                     LDIRNFTAFAENRHPEEVVEYLESLFEFMIEIVSRHRGIVNKFLGDGFMAVFGAPLSD
                     GRDCANAVAAAREILARVDEEVARGSVLPTRVGIGLHAGEAVTGSIGSSLRKEYTVIG
                     DVVNLASRLEQLNKQYGSKLMVSEMVTDRLGGP"
     gene            complement(1281..2930)
                     /locus_tag="AC159P_13555"
     CDS             complement(1281..2930)
                     /locus_tag="AC159P_13555"
                     /inference="COORDINATES: protein motif:HMM:NF019339.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3696520.1"
                     /translation="MKSCPTCQSVYADEALDFCRGDGALLVSSSPPLAETAAETAATW
                     TFPASRPSAETHVGAIPGTPSIAVLPFVNMSSDPENEFFCDGLAEELINALTKVEQMR
                     VVARTSAFSFKGKDAHISRIGQALSVNTVLEGSVRRVGDRLRITAQLIDVADGFHLWS
                     ERYDRRLEDVFEIQDDLSLAIVGALRIKLLGEKKAAVLKHYTADAETYQLYLRGIYHY
                     HRWTEEGYRKAIECFRQVTENEPNHAPAHARLAGAYSQQHFFGHAPPREIVSEWRSAA
                     LKALEIDASLAEAHLAVALFKFNYEWDWAGAEESFRKAIELNPNHAATHEFFPFLLLA
                     LGRFEEAVAEGRRACELDPRSLVGNLNTGWAYWPTGQYDRLIENGRGLLALDPNFFGG
                     HWQAGVGYWLQGKYEEALAEYQRALALSPIPLVRSQLGAFYGLSGRRDMARRVLGELR
                     ELRERRYVPAFDIALVHAGLGETDEAFAWLGKAYEERNGELVHLRFFPRPFSDLAADP
                     RFTQLLRSMRVPTSGAPPHARRQLTANTSLMELESVGGGRT"
     gene            complement(2988..3959)
                     /locus_tag="AC159P_13560"
     CDS             complement(2988..3959)
                     /locus_tag="AC159P_13560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR3696521.1"
                     /translation="MAANSKVTKIESISRTGLAVIYLELDENLKETGKELDDILRARN
                     ISTPGGQVNVEGKNVTIDPSGKFKSGDEIGDVLVPTNAGRAVYLRDIADVVRSYDAPA
                     RYLNYFNWRDEAGEWHRSRAITLSVQMRSGEQIDKFGHAIDETLAGLKQQLPADLIYA
                     RTSDQPLQVKESIDLFMTSLYEAIILGVISLVGVIVSHIIVLFDFIEEKHAEGEPLVE
                     ALPDAGIMRLRPVLITVGATVFALFPLASHGGPLWEPMCYAQISGLCAATFITLLLVP
                     VLYAIFVLDLKLVKWETVGGHAGEDSFDGRQPPSVVEQRDAGVETKL"
     gene            complement(4078..>4322)
                     /locus_tag="AC159P_13565"
     CDS             complement(4078..>4322)
                     /locus_tag="AC159P_13565"
                     /inference="COORDINATES: protein motif:HMM:NF014950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR3696522.1"
                     /translation="RGPELQAPLAELKSSVGPAAGAAPAAPVSRLLSIEEVERAHIEE
                     VLRHTGGAVAGRGGAAEILGLPSSTLRARMKKLGLK"
BASE COUNT          740 a         1421 c         1414 g          747 t
ORIGIN      
        1 cggcccgccc agccgatcgg tcaccatctc cgagaccatc agcttcgagc cgtactgctt
       61 gttgagctgt tccaggcgcg aggccagatt caccacgtcg ccgatgaccg tgtactcctt
      121 ccgcagcgaa gacccgatgc tccccgtcac ggcctctccg gcgtggaggc cgatgccgac
      181 ccgcgtcggc aagacgctgc cgcgggcgac ctcttcgtcc acccgcgcga ggatttcccg
      241 cgccgccgcg acggcgttcg cgcagtcgcg cccgtccgag agcggcgcgc cgaagacggc
      301 catgaaaccg tcgcctaaga atttgttgac gatgccgcgg tggcggctca cgatctcgat
      361 catgaactcg aacagcgact caaggtattc gaccacctcc tccgggtgcc tgttctcggc
      421 gaaggccgtg aagttcctga tgtccagaaa catcacgcac acgtcgcgct tctcgctgcg
      481 cacgtcgtgc ccgcgttcga gcagtttgtc cacgaccgcc ggcgagacgt gctgcccgaa
      541 cgtgttgacg atgtcgttcc gctcctccgc gagcttgaag gaatcgagaa tccgcttctt
      601 tatctgcaag gcgacgaggc cggtgacgac gccgagcgcg agcaggatca cgcctttcac
      661 cgcgtggagc ggcgcgctca ccagcagcgg gttgagcgtc gagccttcgg cccggtcgag
      721 gtagaacatc ccgacgagcg cgtattcgag cgcggcgacg gcccccgtga agacgcacag
      781 cctgaagttc agacgcagcg ccgagaggat gatgaagacc gggtagatga agacggggag
      841 ggcgagggcg gcgaccagat cgtccgtgta cgacgccagc aggatgattt cggcggtcgg
      901 gaaactggtc tcgacgaagg cggtcacgta gcggtagaag ccgggcacct tcctcccgtt
      961 cttgaagaca tagccgagca gaatccgctc cgagaagacg tacagcgcga ggatgaacag
     1021 tatgaggaag cgccagaaga tgaacgcctg catctcgccg cgaaagacgt gctcgatgcc
     1081 ctccgagaaa acggtcgggg gaatcaaaga cagcagcagg ctcaccgtga tgacgatggt
     1141 caggacgagc acgcgcagtc tttccgcgcc gagcgcctcg cgctcgaact cggccttcag
     1201 gatgctctcg gcatcgaccc gcgcttttcc ggcgcggagc ctgagcctgc gtagatgtag
     1261 tgagactgcc gccatctgga ttaagtgcgt cctccaccca cggactcaag ctccatgaga
     1321 gacgtgtttg cggtaagttg gcggcgagcg tgcggcggcg cgccggaggt cgggactctc
     1381 atgctccgca gcagttgcgt gaaccgcggg tcggcggcga ggtcggagaa aggacgcggg
     1441 aagaaccgga gatgtaccag ctcgccgttg cgctcctcgt aagccttgcc gagccacgca
     1501 aacgcttcgt ccgtttcgcc cagcccggcg tggacgaggg cgatgtcgaa ggcgggcacg
     1561 taccgccgct ctctcagctc gcgcaactcg ccgagcacgc gtcgcgccat gtcgcgccgg
     1621 cccgacagcc cgtaaaacgc gcccaactgc gaccgcacca gagggatggg gctgagcgcc
     1681 aacgccctct ggtactccgc gagcgcctcc tcgtacttcc cctgaagcca gtagccgacc
     1741 ccggcctgcc agtgcccgcc gaagaagttc gggtcgagcg cgagcagccc ccgaccgttc
     1801 tcgatcaggc ggtcgtactg gccggtgggc cagtaggccc aacccgtatt caggttgccg
     1861 acgagcgagc gcgggtcgag ttcacacgcg cgcctgcctt cggcgacggc ctcttcaaac
     1921 cttccgaggg ccagtaaaag gaaggggaag aattcgtggg tcgccgcgtg gttcgggttc
     1981 agctcgatgg ccttccggaa cgactcttcg gcccccgccc agtcccactc gtagttgaac
     2041 ttgaacaggg ccacggcgag gtgcgcctcg gcgagcgagg cgtctatttc cagcgccttc
     2101 agcgccgccg accgccactc gctcacgatc tcgcgcggcg gggcgtgacc gaaaaagtgc
     2161 tgctgggagt aggcgccggc cagtctcgcg tgagcgggag cgtggttcgg ctcgttctca
     2221 gtcacctgcc ggaagcattc gatggctttc cggtagccct cttccgtcca acggtggtag
     2281 tgataaatcc cgcgcaggta gagctgataa gtttcggcgt cggcggtgta gtgcttcaag
     2341 accgcggcct tcttctcccc gaggagtttg atcctcagcg cgccgactat cgccagcgac
     2401 agatcgtcct gaatctcgaa gacatcttcc agtcggcggt cgtagcgctc cgaccagaga
     2461 tgaaacccgt cggccacgtc gatcagttgg gccgtgatgc gcagccggtc gccgaccctc
     2521 cgcacgctcc cctccagcac ggtgttcacg ctgagcgcct gaccgatgcg gctgatgtgc
     2581 gcgtctttgc ccttgaagga gaaggcggag gtgcgcgcca cgacccgcat ctgttcgact
     2641 ttggtcagcg cgttgatcaa ctcttcggcc aacccgtcgc agaagaactc gttctccggg
     2701 tcggaactca tgttgacgaa aggcagcacc gcgatggaag gcgtgccggg aatcgcgccc
     2761 acgtgcgtct ctgcgctcgg gcgcgaggcg gggaaagtcc acgtggcagc ggtctcggca
     2821 gcggtctcgg caaggggcgg cgaagagctg acgagcaacg cgccgtcgcc gcggcagaag
     2881 tcgagcgcct cgtccgcgta aacgctctga caggtgggac agcttttcat attgctcaca
     2941 gcctcagcgt ccgcctcttc cgtcggaagg gttgctcggt cgcgccctca tagtttcgtc
     3001 tcgacgcccg cgtcccgttg ttccaccacc gagggcggct gacgcccgtc gaaggagtcc
     3061 tcgccggcgt ggccgccgac ggtctcccac ttcacgagct tcaggtcgag cacgaagatg
     3121 gcgtagagca cgggcaccag cagcagcgtg atgaaggtgg cggcgcacag gccgctgatc
     3181 tgcgcgtagc acatcggctc ccacagcggc ccgccgtgcg aggcgagcgg gaagagcgcg
     3241 aagacggtcg cgccgacggt gatgagcacg ggccgcaggc gcatgatgcc cgcgtcgggc
     3301 agcgcttcga ccagcggctc gccctcggcg tgcttctcct cgatgaagtc gaagagcacg
     3361 atgatgtggc tgacgatgac gccgacgagg ctgatgacgc cgaggatgat cgcctcgtac
     3421 aaactcgtca tgaaaaggtc gatgctctct ttgacctgca acggctggtc ggacgtgcgc
     3481 gcgtagatca agtcggcggg cagttgttgt ttgagtccgg ccagcgtctc gtcgatggcg
     3541 tgcccgaact tgtcgatctg ctcgccggaa cgcatctgca cagagagcgt gatggcgcgg
     3601 ctgcggtgcc actcgcccgc ctcgtcgcgc cagttgaagt aattgaggta gcgcgctgga
     3661 gcgtcgtagc tgcgcaccac gtcggcgatg tcgcgcaggt agacggcgcg gcccgcgttg
     3721 gtcggcacca gcacgtcgcc gatctcgtcg ccgctcttga acttgcccga agggtcgatg
     3781 gtgacgttct tcccctcgac gttgacctgc ccgccgggcg tcgagatgtt gcgcgcgcgc
     3841 aggatgtcgt cgagttcctt gcccgtctcc ttcaggttct cgtcaagctc caggtagatg
     3901 acggcgaggc cggtgcgcga gatggactcg atcttcgtca ccttcgagtt ggccgccatc
     3961 tgctcttcga ccttcttcgt cacgagccgc tcgatgcgct cggcgccgcg ccctctgcgc
     4021 ctcggcgcac tcggcgttac tttttgccct cggtaaaccc aaccacaatc cgcaaggcta
     4081 tttcaacccg agctttttca tgcgggcgcg cagggtcgag gacgggaggc cgagaatctc
     4141 cgccgcgccg ccgcgccccg cgacggcgcc gccggtgtgc cgcaacacct cttcgatgtg
     4201 cgcgcgctcg acctcttcga tggagagcag cctggacaca ggcgccgcgg gcgcggctcc
     4261 ggcggcgggg ccgaccgacg acttcagctc ggcgagcggg gcttgcagct ccggcccgcg
     4321 cg
//
LOCUS       DBNGOH010000700         2935 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3586111, whole genome shotgun sequence.
ACCESSION   DBNGOH010000700 DBNGOH010000000
VERSION     DBNGOH010000700.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2935)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2935)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2935
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3586111"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(226..618)
                     /gene="rpsH"
                     /locus_tag="AC159P_13570"
     CDS             complement(226..618)
                     /gene="rpsH"
                     /locus_tag="AC159P_13570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013581279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HIR3696523.1"
                     /translation="MTDPIADMLTRMRNAIAAKHSRVDIPASKLKLEIARILKEEGYV
                     NNFVVKGEGPRRVIRIFLRYDARGTSSISHLSRVSRPGRRVYLGATDIPKVLGGYGIN
                     IVSTSRGLMSGKRARRENVGGEVLAEIY"
     gene            complement(740..925)
                     /locus_tag="AC159P_13575"
     CDS             complement(740..925)
                     /locus_tag="AC159P_13575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HIR3696524.1"
                     /translation="MAKKSLIAKSKRTPKFSARAYTRCQRCGRPRGYLRKFKLCRICF
                     RELALQGQIPGVVKSSW"
     gene            complement(1008..1553)
                     /gene="rplE"
                     /locus_tag="AC159P_13580"
     CDS             complement(1008..1553)
                     /gene="rplE"
                     /locus_tag="AC159P_13580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HIR3696525.1"
                     /translation="MASRLKERYHGEIAPALAKEFAITNPMAVPRLEKIVINMGLGEA
                     IANAKIVDTAAEELRAVSGQKPVTTRAKKSIAAFKLRQGMPIGSMVTLRGERMYEFLD
                     RLVSIALPRVRDFRGVSPKAFDGRGNYTLGIRDQLIFPEIDFNKVDKTRGMNISIVTT
                     ARDDEQARALLRALGMPFRQS"
     gene            complement(1649..1993)
                     /gene="rplX"
                     /locus_tag="AC159P_13585"
     CDS             complement(1649..1993)
                     /gene="rplX"
                     /locus_tag="AC159P_13585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HIR3696526.1"
                     /translation="MAQKGTKRSKIRKGDQVVVIAGRDNGKRGRVLEVLPSEGKVKVE
                     GAAIVKRHQRPNPQAGRSGGIIDKEAFINISNVQLIDPQTGKPTRARYEVAEDGKKSR
                     VAAGSGHTLDKE"
     gene            complement(1997..2371)
                     /gene="rplN"
                     /locus_tag="AC159P_13590"
     CDS             complement(1997..2371)
                     /gene="rplN"
                     /locus_tag="AC159P_13590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014787185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HIR3696527.1"
                     /translation="MAIQMQTSLDVADNSGARRVEMIQTIGGSTTRTRASLGDKIKVT
                     VKEASPDGAVKKGTVHNAVIVRTRKEVRRKDGTYIRFDQNACVLIKPDDTPIGTRVFG
                     PVARELRDRNFMKIVSLAPEVI"
     gene            complement(2469..2861)
                     /gene="rpsQ"
                     /locus_tag="AC159P_13595"
     CDS             complement(2469..2861)
                     /gene="rpsQ"
                     /locus_tag="AC159P_13595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016463571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HIR3696528.1"
                     /translation="MAENQENGQAAEETAVAEEVKSGQGLAQELATGVDSQAGDATGS
                     EAPRTSRRAEKVGIVASDKMEKTVVVRVDRLVRHPKYRRYVRRTSKFMAHDEKGATIG
                     DKVRIVETRPLSARKRWRVVEIIQKAEK"
BASE COUNT          421 a          984 c          912 g          618 t
ORIGIN      
        1 ccctccggga tcggcgtccg cagcacgccc ttcggcccct tcacctccag ctcgcgctcg
       61 tggatcgtga ccgtcacgcc cttcggcccc gcgatgactt ttttaccgat acgactcatg
      121 acattaacct tcggttaata aggagacagg agacaggaga cgggagacgg aacgaagttg
      181 cggcgaccgc tttgattccg tctcccgact cctgccttcc gtctcctagt agatttccgc
      241 cagcacctcg ccgccgacgt tctcgcggcg ggcgcgcttg cccgacatca ggccgcgcga
      301 ggtcgagacg atgttgatgc cgtacccccc gagcaccttc gggatgtcgg tcgcgccgag
      361 gtagacgcgc cgtccgggcc gcgagacgcg cgagaggtgc gagatcgagg aggtgccgcg
      421 cgcgtcgtag cgcaggaaga tgcggatcac gcggcgcggg ccttcgccct tgacgacgaa
      481 gttgttgacg tagccctcct ccttgaggat gcgcgcgatc tccaacttca gcttcgacgc
      541 cgggatgtcc acccgcgagt gcttcgccgc gatggcgttc cgcatccgcg tcagcatgtc
      601 ggcaatagga tcagtcatta ctcaactcca aaaacggtga caggtgacag gtgacaggat
      661 acaaccccgt ggcgtgaacc tcggagggcg acgcggcgac gggctttttc ttgtcacctg
      721 tcacctgtca cggcttttct taccagctcg acttgacgac gccggggatc tgcccttgca
      781 gcgccagctc gcggaagcag atgcggcaca gcttgaactt gcgcaggtag ccgcgcgggc
      841 gcccgcagcg ctggcagcgc gtgtaggcgc gcgccgagaa cttcggcgtc cgcttcgact
      901 tcgcgatcag tgatttctta gccatgttct ctctcggtga atggtgaatg gtaaatggat
      961 tgaaggcggt tgctattcac cattgaccat ttactattta ctaactccta gctctgtctg
     1021 aagggcatcc cgagcgcccg cagcagcgcg cgggcctgct cgtcgtcgcg cgcggtggtg
     1081 acgatggaga tgttcatgcc gcgcgtcttg tccaccttgt tgaagtcgat ctcggggaag
     1141 atcaactggt cgcggatgcc gagcgtgtag ttgccgcgcc cgtcgaacgc cttgggcgag
     1201 acgccgcgga agtcgcgcac gcgcggcaag gcgatggaga ccagccggtc caagaactcg
     1261 tacatccgct cgcccctgag cgtgaccatc gagccgatgg gcattccctg ccgcagcttg
     1321 aaggccgcga tggatttttt ggcgcgggtc gtgacgggct tctggcccga gacggcgcgc
     1381 agctcttccg ccgccgtgtc cacgatcttg gcgttggcga tggcctcgcc gaggcccatg
     1441 ttgatgacga tcttttcgag ccgcggcacc gccatcgggt tcgtgatcgc gaactccttg
     1501 gcgagcgccg gcgcgatctc cccgtggtaa cgttctttca gtcttgaagc catctcttta
     1561 acctctacct catcttcgct cctgggttcc gggccgccgc ctgtcgatca ggccgcgcgc
     1621 gtttccaggg atgcggtatt ccctcttgct attccttgtc gagcgtatgg ccgctgccag
     1681 cggcgacgcg cgacttcttg ccgtcttccg cgacctcgta gcgcgcgcgc gtcggcttgc
     1741 ccgtctgcgg gtcgatcagt tgcacgttcg agatgttgat gaaggcttcc ttgtcgatga
     1801 tgccgcccga gcggcccgcc tgcgggttcg ggcgctggtg gcgcttcacg atcgcggcgc
     1861 cctcgacctt gaccttaccc tccgagggca gcacttcgag cacgcgcccg cgcttgccgt
     1921 tgtcgcggcc cgcgatgacg acgacctggt cgcccttcct gatcttcgaa cgcttggttc
     1981 ccttctgtgc catcgcttaa atcacctccg gcgcgagcga aacgatcttc atgaagttgc
     2041 ggtcgcgaag ctcacgcgcg acggggccga agacgcgcgt cccgatgggc gtgtcgtcgg
     2101 gcttgatgag cacgcacgcg ttctggtcga agcggatgta ggtgccgtcc ttgcggcgca
     2161 cctccttgcg cgtgcggacg atgacggcgt tgtgcaccgt ccccttcttg accgccccgt
     2221 ccgggctggc ctctttgacc gtcaccttga tcttgtcgcc gaggctcgcc ctcgtccgcg
     2281 tagtcgagcc gccgatggtc tggatcatct cgacgcgccg cgcccccgag ttgtccgcca
     2341 cgtccagcga cgtttgcatc tgaatagcca tgtgctcact ccgttcgcgt gaaccgtgaa
     2401 ccgtgaaccg taacaagtcg aaagccggtt ccgtcttgtc acggtttacg gtttaccgtt
     2461 gacggctttt acttttccgc cttctggatg atctcgacga cgcgccagcg cttgcgggcc
     2521 gagaggggcc gcgtctcgac gatccgcacc ttgtcgccga tggtcgcgcc cttctcgtcg
     2581 tgcgccatga acttcgaggt gcggcggacg tagcggcggt acttcgggtg gcgcacgagg
     2641 cgatccacgc ggacgacgac cgtcttctcc atcttgtcgg aggcgacgat gccgaccttc
     2701 tcggcgcggc ggctggtgcg cggcgcctcg ctgcccgtcg cgtcgcccgc ctgcgagtcc
     2761 acgcccgtcg ccaactcctg cgcgaggccc tgccccgact tcacctcttc ggcgaccgcc
     2821 gtctcttccg ccgcctgccc gttttcctga ttctctgcca ttgctcactc cgttcggggg
     2881 ccgggtgtcg ggggccgggg gccggtttaa agccggtctt caccggcccc ccgcc
//
LOCUS       DBNGOH010000701        11102 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2670561, whole genome shotgun sequence.
ACCESSION   DBNGOH010000701 DBNGOH010000000
VERSION     DBNGOH010000701.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 11102)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11102)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11102
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2670561"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..504)
                     /locus_tag="AC159P_13600"
     CDS             complement(<1..504)
                     /locus_tag="AC159P_13600"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010323228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase family protein"
                     /protein_id="HIR3696529.1"
                     /translation="MEDSILKAPKAELHIHIEGSMEPELMFGLAARNRLPLPFASVEE
                     ARRSYDFRDLQSFLDIYYQGARVLLHEQDFYDLTWAYLRRSAAQNVRHAEIFFDPQTH
                     TARGVPFGAVVGGINRALAEGAEQLGITSKLIMCFLRHLSADAAMATLDEALRHEGMI
                     AGVGLDSS"
     gene            752..1315
                     /locus_tag="AC159P_13605"
     CDS             752..1315
                     /locus_tag="AC159P_13605"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR3696530.1"
                     /translation="MEDSRADEELVEFVLAGQDEVFARLYERYYARTHRVAYVMTGTR
                     EEAENLTQEVFMLAYRKLRQFGGRSSFSTWFYRLTVNCCLSHRKRERKFLGARIDDVE
                     GLDLPRCGESAEAEILRQQIQRHVHTALLSLKPKLRMVVILKDVEELSYEQIAERMGC
                     STGTVGTWLNRARKLLARKLEGLKGKL"
     gene            1318..3522
                     /locus_tag="AC159P_13610"
     CDS             1318..3522
                     /locus_tag="AC159P_13610"
                     /inference="COORDINATES: protein motif:HMM:NF013507.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HIR3696531.1"
                     /translation="MKLGGIRRVFGGGCARDCRPLLSPYVDGELAPHLRARVESHLAR
                     CPRCRREYDEILFAARAVSCLSPPTTPTTLPLWLRQNGRGDSRHAGRPASTWRHARRW
                     APLGAAAAGLLLLFAGASLWYRTRTAPRAEWEVTRLAGTPSVNSRAVEKSSRFGVGDW
                     VETDDSSRALIAVGLIGHVEVDVGTRARLVEAGPSAHRLELVRGRMDAAIIAPPRLFF
                     IDTPSATAVDYGCAYTLEVDDAGASLLHVTAGWVSLAVAGGRESLVPAGAMCRAEPGK
                     GPGTPFAQDASRALREALDEFDFGHGGDAALDAVLGEARAKDALTLWHLLERAEGSAR
                     ERVYERLAALVPPPAGVTREGVLSLDRRMLDGWRESVDYASVGIDPAKVPTATGSLRP
                     TGTTNDPRFAHTATPLSDGGVLVAGGLEREGVPLSSAEIYEPERGTFARVGSMRSRRV
                     GHTATPLRDGTVLLAGGSEESFYEGASSSAELYDPQARAFVPLADMGAPRLAHRATLL
                     ADGRVLITGGQDDTGRKLSSAEIYDPATRAFTPAGRMATSRSDHTATLLKNGLVLVAG
                     GATGRYPGEEPVASAELYDPATNTFKPTGGLSMVRYKHSATLLPDGKVIVVGGSDARM
                     WGGKYDSAEIYDPAAGAFNSTGRMSTSRYKIRDAVVPLRDGRLLVAGGGAHAEVYDPA
                     TGVFGLVKGGSSVPRYYSTATPLRSGEVLIVGGYLGVNRWLQADASAWVYRP"
     gene            3633..4340
                     /locus_tag="AC159P_13615"
     CDS             3633..4340
                     /locus_tag="AC159P_13615"
                     /inference="COORDINATES: protein motif:HMM:NF012976.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dioxygenase family protein"
                     /protein_id="HIR3696532.1"
                     /translation="MNSKRREFLQRSGMSLAAVGVGGLVLRADALPTRAAPSEEAETG
                     RVLIQMEPPPKELTKASGELKKITPVVYGPFYRPGAPFRGKLCPPGEPGTTFVLTGRV
                     WAFDTKRPLPGVVLDIWHVDMKEQYSDGVKDSRNRGRLVSSETGAYEVEGIRPIPYRP
                     NPSGDFWRCAHFHLLAVLPGYKPLVTEIHFKDDPKKNDPMYRPENAIAVERHTANGKT
                     YETGVFDIVLERAADSK"
     gene            4389..5045
                     /locus_tag="AC159P_13620"
     CDS             4389..5045
                     /locus_tag="AC159P_13620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012232797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HIR3696533.1"
                     /translation="MNRLLAVVALLALAAASSACANPAADAPKAKTSEATTRPTARAA
                     DAAGERLPITNENSRIEFVGSKVTGSEGGRFEKFTGTVHLVEARPERSRVEIAIDMNS
                     VVTDSSGLAEHLKSADFFDAPKFPTATFISTDIKPGGEKGATHTVTGTLDLHGVTKAI
                     AFPATIRVDADAASLDAVFAINRKDFRITYAGRTNDLIRDEVVIKLYVRAPRAGTESN
                     "
     gene            complement(5120..10519)
                     /locus_tag="AC159P_13625"
     CDS             complement(5120..10519)
                     /locus_tag="AC159P_13625"
                     /inference="COORDINATES: protein motif:HMM:NF019298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HIR3696534.1"
                     /translation="MSTARPIRRTRAGLTLILFAIAALLYTAPPRSGVAAAGDSISAT
                     YLDGTLRLSVPYDEPVARSRSLRVEVLSPDDKLVAGATKPVAPARETGPWDVSVSLSP
                     KMPLEDLLWHRARIGTKMVSLSEILRLPVVRVLAQRTYAAGARASLRVVAAEAKTGDP
                     LRDSRVKLELVHGETAVALFEGMTDALGTAEVAFTVPPAGFGPRTLRVTAETPLGPVT
                     AHQPVQIERRNKILLTTDKPLYQPGQTMHLRALALDASTRAAAFDQPLTLEVEDAKGN
                     KVFKRRGRTDRFGIASADFELADEVNFGPYHVRAILGEDAAVYTQEKTVTVDRYVLPR
                     FKVEIGLSKDAGREQQSYYSPGETVEGEVTARYLFGKPLAGAAVTVVLKAFDVEASEI
                     GRLTGKTDAEGRFPFSSRLPDFLAGRSTQQGSAPVSVGVEIKDTADHTETKSRDILVS
                     NTPILIMAVPESGQLLPGLENRVFVLTSYPDGRPAETTVSGSLLGSPLKTDESGVAVI
                     PVRAATGAMQLKLRAADAQGRAGEATIRLEARSQSESLMLRTGRAVLKVGDKLRLETI
                     STRERGAVYVDVVKDGQTLLTRALETEGGRGRLDIDLTPEMFGAVEVRAYQFTADADP
                     ISDRKLVYVDPADDLRIAVSAARESYQPGEDARIDFRATDTAGRPVSAALGVEIVDEA
                     VFALSDKQPGFEKVFMHLQKELLTPRYEVHQFSFEQVVLDDFQGEVAGRTGRRERAAE
                     VLLAAAGTVADRDVRGLYGREPYEVKRHEYSARYTRRVAERAQALVPAMTKYYEDHPA
                     SKEGFGRDLQLFASTETGRGLTLTDPWGNPFTGEGAFGAHDSNVYFTLRSAGPDGRHG
                     TADDVGIPIHAQRDGPRERVRTTRFKGSATTEEGVVGGGVVEVVGAVKDEHGTPVSRV
                     KVLALRVSDGKAVWVYTNPRGHFAIAGLPPGNYRVTFESESHHTTTYKTLALGAGARG
                     RVETVLATRGPRGVRLSLYNSYYGIDGVAETVTVTADGAGGGGLRRRQIRDLPVNARR
                     VDMLMMEAPAAKEKAAGAADLLSDDKDARGGGAKKEDGGGAGPRVRSFFPETLYTNPA
                     LITDGDGRASVNVPLADSITTWRVTSLASTAAGALGSSTAPLRVFQDFFIDLDLPVSV
                     TQGDEVSVPVAVYNYLPSPQSVSLELRQDSWFTLEGGDTAFKQVEVGAGEVTVAHFRL
                     KASKIGEQQLQVTGRLGARTSEAGDAAGTPAGDAVARNLTVVPNGEERLVVHNERLEH
                     SAAREVSIPEEAIPDASKIFVKFYPGALSQMVEGLDSILRMPGGCFEQTSSSTYPNVL
                     VLDYLKTSKKLTPEIQAKAEGYISLGYQKLVTFEVKGGGFSWFGNDPANKILTAYGLM
                     EFSDMSRVHEVDPRVIERTQAWLASQQQPDGSFKPDTYFINEGATTRYNTGLVRITAY
                     IGWALASTGYTGAAVEKAKQYVRSHTTGEEDAYTLAVVANFAADSGTDKAWTESAVGT
                     LASRASDDGKLAFWRQEGETPTSAREDSADLETTALAVQALLKSGQGGGLAKKGLDYL
                     TSKKDALGNWQTTQATILALKAFLLSHTRGSNTDTEGTINVSVDGRPAGSVQVTRDNN
                     DLLHLVDLKQFTHTGANRIALSFVGRGSMQYQIVGRYYVPWSRVADRGGKGRGAEPLS
                     IDLAYDRTRLAQNETVTARVRVQNNTAAKAKMVMIDLGIPPGFEPLGEDFAELLDTTR
                     GKLGGKIEKYTITAKQVILYLDGLNARQITEFNFRLRAKFPLRAQTFPSRVYEYYNPG
                     SGDSTKPSTLVVTAK"
BASE COUNT         1769 a         3647 c         3822 g         1864 t
ORIGIN      
        1 ggacgagtcg aggccgaccc ccgcgatcat cccctcgtga cggagcgcct cgtcgagcgt
       61 cgccatcgcc gcatcggcgc tcaggtgccg gaggaagcac atgatgagct tcgacgtgat
      121 gccgagctgc tccgcgccct cggccagtgc gcggttgatg ccgcccacga ccgcgccgaa
      181 ggggacgccg cgggcggtgt gggtctgagg gtcgaagaag atttcggcgt ggcgcacgtt
      241 ctgcgcggcg ctgcgccgca ggtaggccca cgtcaggtcg tagaagtcct gctcgtggag
      301 cagcacgcgc gcgccctgat agtagatgtc gaggaaggac tggaggtcgc ggaagtcgta
      361 ggagcggcgc gcctcctcga ccgaggcgaa cggcagcggg aggcggttgc gggccgcgag
      421 accgaacatc agctccggct ccatcgatcc ctcgatgtgg atgtgcagct cggccttcgg
      481 ggccttgaga atcgaatctt cgatgtcgag cacggagcgc ctccccggta gagacccttg
      541 acggacgttg atgtctttag cgcgtcgggg cgacaatcat acatcagccc ggctccacga
      601 cgtgcccatc ccggtgccga aagccgcacg atcttaccgg atgaaacacg gtttcgccgc
      661 ttcccggaaa gtgtgaaaag aatgaaagaa cgagccgccg gctgctgtct aagtgagatg
      721 tgtgagtaac gtgtgcaaga aggccgccta cttggaagac agtcgggccg atgaagagct
      781 ggtggagttc gtgctcgccg ggcaggacga ggtgttcgcg cggctctacg agcgctacta
      841 cgcgcgcacg caccgcgtcg cctacgtgat gacggggacg cgcgaggagg ccgaaaacct
      901 cacgcaggaa gtcttcatgc tggcttatcg gaagctccga cagttcggtg gccgttcgag
      961 cttctcgacg tggttttacc gtctgacggt gaactgctgc ctgagccacc gcaagcgcga
     1021 gcggaagttt ctcggggcga ggatcgacga tgtcgagggc ctcgacctgc cgcgctgcgg
     1081 cgagtccgcc gaggcggaaa tcctccggca gcagatacag cggcacgtcc acacggcgct
     1141 gctgagcctg aaaccgaaac tgcggatggt ggtcatcctg aaggatgtcg aagagttgag
     1201 ctacgaacag atcgccgaac ggatgggttg ttcgacgggc acggtgggga cgtggctgaa
     1261 ccgtgcgcgg aaactgctgg cgcggaagct ggaagggttg aaggggaagc tttgacgatg
     1321 aagctcggcg ggatcaggcg agtcttcggc ggcggttgcg cgcgcgactg ccgccccctg
     1381 ctctcgccgt acgttgacgg cgaactcgcg ccgcacctgc gcgcacgcgt cgagtcacac
     1441 ctcgcgcggt gcccgcgctg ccggcgcgaa tacgacgaaa tccttttcgc cgcgcgcgcc
     1501 gtctcgtgcc tttcgccgcc caccaccccc acgacgctgc ccctctggtt aaggcagaac
     1561 gggcgcggcg acagccgcca cgccgggcgg cccgcatcga cgtggcggca cgcgcggagg
     1621 tgggcgccgc tcggggccgc ggcggcgggg ctgctgctcc tcttcgcggg cgcgagtctc
     1681 tggtatcgca cgcgcacggc gccgcgcgcc gagtgggagg tgacgcgcct cgcgggcacg
     1741 cccagcgtca actcgcgcgc ggtggaaaag tcgagtcgct tcggcgtcgg cgactgggtc
     1801 gagacggacg actcttcgcg ggcgctgatc gcggtcgggc tgatcgggca cgtcgaggtc
     1861 gatgtcggca cgcgcgcacg gctcgtcgag gcaggcccct cggcgcaccg cctcgaactg
     1921 gtgcgtggcc ggatggacgc cgccatcatc gcgccgccga gactcttctt catcgacact
     1981 ccttcggcca ccgccgtcga ttacggctgc gcttacacgc tcgaagttga cgacgcaggg
     2041 gcgagcctgc tgcacgtcac ggcggggtgg gtcagcctcg cagtggcggg cgggcgcgag
     2101 tcgctggtgc ccgccggggc gatgtgccgc gcggagccgg ggaaggggcc gggcacgcct
     2161 ttcgcgcaag acgcttcgcg cgcgctgcgc gaggcgctcg acgaattcga tttcgggcac
     2221 ggcggagacg ccgccctcga cgcggtgctc ggcgaagcgc gggccaaaga tgcgctcacg
     2281 ctctggcacc tgctggagcg cgccgaaggc tctgcgcgag agcgcgtcta cgaacggctg
     2341 gccgcgctcg tgccgccgcc cgcgggcgtc acgcgtgagg gcgtcttgag tctcgaccgc
     2401 cgtatgctcg acggttggag ggagagtgtg gattacgcga gcgtcggcat cgacccggcg
     2461 aaggtgccga ccgcgacggg gagcctgcga cccacgggga cgacgaacga cccgcgtttc
     2521 gcgcacacgg cgacgccgct ctcggacggt ggggtgctcg tcgcgggcgg tctcgaacgc
     2581 gagggcgtcc cgctgtcgag cgcggaaata tacgagccgg agcgcgggac gttcgcccgc
     2641 gtcggctcca tgcggtcgcg gcgcgtcggc cacaccgcca cgccgttgcg cgacgggacg
     2701 gtgctcctcg ccggcggttc cgaagagtcc ttctacgaag gcgcctcgtc gagcgcggaa
     2761 ctttacgacc cgcaggcccg cgccttcgta ccgctcgcgg acatgggcgc gccgcgcctc
     2821 gcacacaggg cgacgctcct cgccgacggc agggtgctca tcaccggcgg gcaggacgac
     2881 acggggcgca agctgtcgag cgccgaaatc tacgacccgg cgacgagggc tttcacgccc
     2941 gcgggccgga tggcgacctc gcgctccgac cacacggcga ccctcctgaa gaacgggctg
     3001 gtactcgtcg cgggcggggc gacgggccgc taccccggcg aggagccggt cgcgagcgcg
     3061 gagctttacg accccgccac caacacgttc aagccgacgg gcggcctgag catggtgcgc
     3121 tacaaacact ccgcgaccct gctgcccgac ggcaaggtga tcgtcgtcgg cgggtcggac
     3181 gcgcgcatgt ggggcgggaa gtatgacagc gccgaaatct acgacccggc ggcgggcgcg
     3241 ttcaactcca cgggccgcat gagcacgtcg cgctacaaaa tacgggatgc ggtcgtgccc
     3301 ttacgcgacg ggcggctcct cgtcgccggg ggcggcgcgc acgcggaagt ttacgacccg
     3361 gccacagggg tcttcggcct ggtcaagggc ggcagcagcg tcccgcgcta ctactcgacg
     3421 gcgacgccgc tccggagcgg cgaggtgctc atcgtcggcg gctacctcgg cgttaaccgg
     3481 tggttgcagg ccgacgcgag cgcctgggtt taccgtccat gaaagagaac gtaatccgcg
     3541 gaaaagtcca agaattcgtg ggggttcgcg gttaacgacc cttcacgatc caacgtcctt
     3601 gctgacgtga atcgacaacc gaggtgcaca tcatgaattc caaacgtcgt gagtttttac
     3661 agcggtcggg catgagtctg gccgcggtcg gagttggcgg tctggtcttg cgcgccgacg
     3721 cgctccccac ccgcgccgcg ccgagcgagg aggcggaaac gggtcgcgtc ctgatccaga
     3781 tggagccgcc cccgaaagag ttgacgaagg cttcgggcga gttgaaaaag attacgccgg
     3841 tggtttacgg gccgttctac aggccgggcg cgccgttccg cggaaagctc tgtccgccgg
     3901 gcgagccggg gacgactttc gtgctcacgg gtcgcgtctg ggcgttcgac acgaagcgcc
     3961 cgctgccggg cgtggtgctc gacatctggc acgtcgatat gaaggagcag tactcggacg
     4021 gcgtcaagga ttcgaggaac cgcgggcggc tggtgtcttc ggagacgggc gcctacgagg
     4081 tcgaaggcat ccgccctatc ccttaccgcc cgaacccttc gggggacttc tggcgctgcg
     4141 cgcactttca cctgctcgcc gtccttcccg gctacaagcc tctggtgacg gaaatccatt
     4201 tcaaggacga ccccaaaaaa aacgacccga tgtatcgccc cgagaacgcc atcgccgtcg
     4261 aacggcacac ggcgaacggc aagacctacg agaccggcgt cttcgacatc gtgctggagc
     4321 gcgccgccga ctcgaaatga acgcggccca agatcgtcgg tcttgagaac acattcgagg
     4381 agaatcacat gaaccgcctg cttgccgtcg tcgccctgct cgccctcgcc gcggcttcgt
     4441 ccgcctgcgc gaaccccgcc gccgacgcgc cgaaggccaa gacctccgag gcgaccaccc
     4501 gaccgacagc ccgcgccgcc gacgccgcgg gcgagcgact ccccatcacg aacgagaatt
     4561 cccggatcga gttcgtcggc tccaaagtga cgggcagcga gggcgggcgc ttcgagaaat
     4621 tcacgggcac ggtgcatctc gtcgaggccc ggcccgaacg gagccgcgtc gagattgcca
     4681 tcgacatgaa ctcggtcgtc accgactcaa gcggcctcgc ggagcacttg aaaagcgccg
     4741 acttcttcga cgcgccgaag ttccccacgg ccaccttcat ctccaccgac atcaagccgg
     4801 gcggcgagaa aggcgcgacc cacaccgtca cgggcaccct cgacctgcac ggcgtgacga
     4861 aggccatcgc cttcccggcc acgatcagag tggacgccga cgccgcctcg ctcgacgccg
     4921 tgttcgccat caaccgcaaa gacttccgta tcacgtacgc gggccgcacc aacgacctga
     4981 tccgcgacga agtggtcatc aagctatacg tccgcgcgcc gcgggccggg accgaaagca
     5041 actgattcgg agtgccgccg cgatagtagc tcttcgcgac aacatctcca gccgcgccga
     5101 agtctgtgcg gcccctcctc tacttcgcgg tcacgaccag cgttgacggc ttcgtgctgt
     5161 cgccggagcc ggggttgtag tactcgtaca cacgggacgg gaaggtttgc gcgcgcaagg
     5221 ggaatttggc acgcagacgg aagttgaatt ccgtgatctg tcgggcgttc aggccgtcga
     5281 ggtacaggat tacctgtttc gcggtgatgg tgtacttctc gattttgccg ccgagtttgc
     5341 cgcgcgtggt gtcgagcagt tccgcgaagt cttcgccgag gggttcgaag ccgggcggta
     5401 tgccgaggtc gatcatcacc atcttggctt tggccgccgt gttgttctgg acgcggacgc
     5461 gggccgtcac ggtctcgttc tgcgccagac gcgtgcggtc gtaggcgagg tcgatcgaga
     5521 gcggttccgc gccccgacct ttcccgcctc tgtcggcgac gcgcgaccac gggacgtaat
     5581 agcgtccgac gatctggtac tgcatgctgc cgcgtccgac gaacgacaac gcgatccggt
     5641 tcgcgcccgt gtgcgtgaat tgcttcaggt ctacgagatg gagaaggtcg ttgttgtcgc
     5701 gcgtgacctg aacggagccg gcgggtcgtc catcgaccga gacgttgatc gtgccttcgg
     5761 tgtcggtgtt cgagccgcgc gtgtgggaga ggaggaacgc tttcagcgcg aggatcgtcg
     5821 cctgcgtcgt ctgccagttc ccgagcgcgt ccttcttcga ggtcaggtag tcgagacctt
     5881 tcttcgccag cccgccgccc tgaccggact tcaagagcgc ctgcaccgcg agcgccgtcg
     5941 tctccagatc ggctgagtcc tcgcgcgccg aagtcggggt ctcgccctcc tgcctccaga
     6001 acgccagctt cccgtcgtcg ctcgccctcg acgcgagcgt gccgaccgcc gactccgtcc
     6061 acgccttgtc cgtcccgctg tcggcggcga agttggcgac gaccgcgagc gtgtaggcgt
     6121 cctcctcgcc ggtcgtgtgc gaccggacgt actgcttcgc cttctcgacc gctgcgcctg
     6181 tgtagccggt cgaggcgagc gcccagccga tgtaggcggt gatgcgcacg aggccggtgt
     6241 tgtagcgcgt cgtcgcgccc tcgttgatga agtacgtgtc cggtttgaag ctcccgtccg
     6301 gttgctgctg cgaggcgagc cacgcctgcg tccgctcgat cacgcgcgga tcgacttcgt
     6361 ggacgcgcga catgtcggag aactccatca gcccgtaggc cgtcaggatt ttgttcgccg
     6421 ggtcgttgcc gaaccacgag aagccgccgc ccttgacctc gaacgtgacg agcttctggt
     6481 agccgagcga gatgtaaccc tcggccttcg cctgtatctc cggcgtcagc ttcttgctgg
     6541 tcttgaggta gtcgaggacg agcacgttcg ggtacgtcga tgaggatgtc tgctcgaagc
     6601 agccgcccgg catccgcagg atcgagtcga gtccttcgac catctgcgac agcgcgccgg
     6661 ggtagaactt gacgaatatc ttcgacgcgt cggggatggc ctcttccgga atcgagacct
     6721 ctctggccgc cgagtgttcg agccgttcgt tgtggacgac cagccgctcc tcgccgttcg
     6781 ggacgaccgt caggttgcgc gccaccgcat cgcccgcggg cgtgcccgca gcgtcccccg
     6841 cctccgacgt tctcgcgccg agtcgcccgg tcacctgtaa ctgctgttcg ccgatcttcg
     6901 acgccttgag acggaagtgt gcgaccgtga cttcgcccgc gccgacctcc acctgcttga
     6961 acgcggtgtc gccgccctca agcgtgaacc acgagtcctg cctcagctca agcgacacgc
     7021 tctgcgggga aggtagatag ttatagacgg cgacgggcac cgacacctcg tcgccctgcg
     7081 tgaccgagac gggcaggtcg aggtcgatga agaagtcctg aaagacgcgc agcggcgcgg
     7141 tcgaggagcc gagcgcgccc gccgccgtcg aggcgagcga cgtgacgcgc cacgtcgtga
     7201 tcgagtccgc gagggggacg ttgaccgagg cgcgcccgtc gccgtcggtg atgagcgcgg
     7261 ggttcgtgta gagcgtctcg gggaagaagc ttcggacgcg cggccccgcg ccgccgccgt
     7321 cctccttctt cgcacccccg ccccgcgcgt ctttgtcgtc ggagagcagg tcagccgcgc
     7381 ccgccgcctt ctccttcgcc gcaggcgctt ccatcattaa catatcgacc cgccgggcat
     7441 tgaccggaag gtctctgatc tgcctcctgc gcaacccgcc gcctccggcg ccgtcggcgg
     7501 tgaccgtcac cgtctcggca actccgtcga tgccgtagta agagttgtac agcgagagcc
     7561 tgacgccgcg ggggccgcgc gtcgcgagca cggtctcgac cctgccgcgc gccccggcgc
     7621 ccagtgccag cgtcttgtac gtcgtcgtgt ggtgcgactc gctctcgaag gtgacgcgat
     7681 agttgcccgg cggaagaccg gcaatcgcga agtggccccg cgggttggtg taaacccaga
     7741 cggccttgcc gtcggagacg cgcagcgcga gcaccttgac gcgggagacg ggcgtcccgt
     7801 gttcgtcttt gaccgcgccc acgacctcga cgacgccacc cccgacgacc ccttcctccg
     7861 tcgtcgcgct ccctttgaaa cgggtcgtgc gaaccctctc gcgcgggccg tcgcgctgcg
     7921 cgtggatggg aatgccgaca tcgtcggctg tcccgtggcg cccgtcgggg ccggctgagc
     7981 ggagcgtgaa atagacgttg gagtcatgcg cgccgaacgc gccctcgccg gtgaaggggt
     8041 tgccccacgg gtctgtgagc gtgaggccgc gtcccgtttc cgtggacgcg aacaactgga
     8101 ggtcgcgtcc gaagccttcc ttcgacgccg ggtgatcttc gtagtatttc gtcatcgcgg
     8161 gcacgagggc ttgcgcccgt tcggcgacgc gccgcgtgta tcgcgcggag tactcgtgac
     8221 gcttgacctc gtaaggctcg cgtccgtaca ggccgcgcac gtccctgtcg gcgaccgtcc
     8281 cggcggcggc gaggagcacc tcggcggcgc gctcgcggcg tcccgtgcgg cccgcgacct
     8341 ctccctgaaa gtcgtcgagc acgacctgct cgaaagagaa ttgatgcacc tcgtagcggg
     8401 gcgtcaggag ttctttctga agatgcatga agaccttctc gaaccccggc tgcttgtccg
     8461 acagggcgaa gaccgcttcg tccacgatct cgaccccgag ggccgccgag acggggcgtc
     8521 ccgcggtgtc cgtcgcgcga aagtcgatgc gcgcgtcttc gcccggctgg tagctctccc
     8581 gcgccgccga cacagcgatg cgaaggtcgt ccgccggatc gacgtagacg agcttgcgat
     8641 ccgaaatcgg gtcggcgtcc gcagtgaact ggtaggcgcg aacctcgacg gcgccgaaca
     8701 tctcgggcgt caggtcaatg tcaaggcgac cgcgtccgcc ctccgtttcg agggcgcgcg
     8761 tgaggagcgt ctgcccgtct ttgaccacat cgacgtagac cgccccgcgc tcgcgcgtcg
     8821 aaatggtttc gaggcgcagc ttatcgccga ccttgagcac ggcccgtccc gtccggagca
     8881 tcaggctctc cgactgcgag cgggcttcga gcctgatggt cgcctcgccc gcgcggcctt
     8941 gcgcgtcggc ggctctgagc ttgagctgca tggccccggt cgcggcgcgg acgggaatga
     9001 cggccacgcc gctctcgtcg gtcttgagcg gcgagccgag gagactgccg ctgacggtcg
     9061 tctccgccgg cctcccgtca gggtatgagg tcaggacgaa cacgcggttt tcaagaccgg
     9121 ggagcaactg gccgctctct ggaacggcca tgatgaggat gggggtgttc gagacgagga
     9181 tgtcgcgcga cttcgtctcg gtgtggtctg ccgtgtcttt gatttcgacg ccgaccgaga
     9241 ccggcgcgct cccttgctgc gtcgagcgac cggcgaggaa gtccggcagc cgcgacgaga
     9301 acggaaagcg gccttcggcg tcggtcttcc ccgtgaggcg tccgatctcg gacgcctcta
     9361 cgtcgaaagc tttgagcacc acggtcacgg cggcgcccgc cagcggcttg ccgaacaggt
     9421 agcgggccgt gacctcgccc tcgacggttt cgcccggcga ataatagctc tgctgctcgc
     9481 ggcctgcgtc cttgctcagc ccgatctcga ctttgaacct cggcaggacg tagcgatcaa
     9541 ccgtgacggt cttctcctgc gtgtagacgg cggcgtcttc gccgaggatg gcgcgcacgt
     9601 ggtaggggcc gaagttgacc tcgtcggcca gctcgaagtc ggcggatgcg atgccgaagc
     9661 ggtcggtgcg tccccggcgc ttgaagacct tgttgccctt cgcgtcttcg acttcgagtg
     9721 tcagtggttg gtcgaaggcg gcggcgcgcg tggaagcgtc gagcgcgagc gcgcgcaggt
     9781 gcatcgtctg gcccggctga taaagcggct tgtccgtcgt caacagtatc ttgttccggc
     9841 gttcgatctg gacgggctgg tgcgcggtga cggggccgag cggcgtctcc gccgtgacgc
     9901 gcagagtgcg cgggccgaag cccgcgggcg ggacggtgaa cgcgacctca gccgtgccga
     9961 gtgcgtccgt cataccctcg aagagtgcga ccgcggtctc gccatgaacg agttcgagct
    10021 tgacgcggct gtcgcgcagc gggtcgccgg tcttcgcttc agccgcgacg acccggaggg
    10081 aagcgcgcgc gcccgccgcg tacgttcgct gggccaggac gcgcacgacc ggcagacgca
    10141 gaatctcgga aagcgaaacc atcttcgtcc cgattctcgc gcggtgccac aacaggtctt
    10201 cgagaggcat cttcggggac aggctgacgg agacatccca cggccccgtc tcgcgcgcgg
    10261 gggccacggg cttcgtggcc cccgcgacga gtttgtcgtc gggcgagagc acttcgacgc
    10321 ggaggctgcg ggagcgtgcg accggctcgt cgtaagggac gctcaaacgg agcgtgccgt
    10381 caaggtaggt cgccgagatg gaatcgccgg cagccgccac accgcttctc gggggcgcgg
    10441 tgtagagaag cgcggcgatg gcgaaaagaa tcaacgtcag ccctgcgcgc gtgcggcgta
    10501 taggtctcgc ggtactcatg ggacatctcc tgtagggtcg agtgcggacg agtccgtccc
    10561 cacgtccttc atgcgggcgt cgggtgagcg ctgtcagtcc ttgccggggt ttcagcgcct
    10621 cgggttcgtg ccttcggtag aacgtaaaaa cggggagacc ttgcgcgtga gtctgcggag
    10681 ggggctgaca tcagcgggag agacgacgcg tgccgtgggc tgcctccgta agaggagctg
    10741 gcctccgatt cagactgttc gccgctgtga tggtggccgg agggtaaaga gttgtgtgag
    10801 ggtggagggg cattcggcgg cgcgcgttcg gagtctgtcg caaaagcgcg gccccggtcg
    10861 gaagtcctcg cacgtccgtg cggacgaggc cggctcaagg tcgcactcga accggggcga
    10921 ggctttttag cagcaggtaa gcggaaggca cgccgactat aaaaaaatct gtagaaaatc
    10981 acggcccgcg cgttgtatat tcatcgatgt ggcttggtgc tcgcgctacg agcaaaaggc
    11041 cgccgggttt taacccgtaa tccgaatgcc tgcttgctcg ccccggctac catattccct
    11101 ca
//
LOCUS       DBNGOH010000702         2695 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_836924, whole genome shotgun sequence.
ACCESSION   DBNGOH010000702 DBNGOH010000000
VERSION     DBNGOH010000702.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2695)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2695)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2695
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_836924"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..522)
                     /locus_tag="AC159P_13630"
     CDS             complement(<1..522)
                     /locus_tag="AC159P_13630"
                     /inference="COORDINATES: protein motif:HMM:NF012708.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HIR3696535.1"
                     /translation="MALLHITDASGRQWECPLTPQGLCTIGRAPDNTVVLNDPRASRY
                     HAHVKFQDGAYILVDGKVGGGRSANHVFVGGEQRFEHPLADGDRVTIGASHLKFERAA
                     RPEQSDADVRYDDKPLGHTQLLVSANDVIRSALAARTTTTTSGSHRAAAAAALSEELE
                     ALRGKAKILALLYD"
     gene            815..1039
                     /locus_tag="AC159P_13635"
     CDS             815..1039
                     /locus_tag="AC159P_13635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696536.1"
                     /translation="MSIAHELSCDVATAMLANPADEQRGAAGGALTDIVLEVHTTLRR
                     LTNEARQRRAQAFRPEAPPNAAGTAASGGY"
     gene            1229..>2695
                     /locus_tag="AC159P_13640"
     CDS             1229..>2695
                     /locus_tag="AC159P_13640"
                     /inference="COORDINATES: protein motif:HMM:NF028240.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative porin"
                     /protein_id="HIR3696537.1"
                     /translation="MRQLALRALLLCTLGSALSMATAAQQQPPPQNTDAGAVKAAPDA
                     ADKTGAAVVDGNPLGAEAIERRLREQREEIESLRALVLEQTRLVKELKGRVERAEQQQ
                     RQAAQQPAQAVFRDASYAAALPSSNAGTTPAERETAGAAQDAKLEERVARVEKASEAV
                     TKQLAGITFFGDLRFRYESIYGQQNALASADTPGALGNPLTPRQRLRLRARFGARGTI
                     GKEFEWGIRFATGSFADTIASNQTLTDFYNRKSFALDNAYLTWKPSRAPGLQVQGGKF
                     ETPWVRTEMTWDNDLNVEGLHESYTRTFEKSKLKRFDLIAWQLPMLERNSAFVLNADG
                     TVDFSASERGGRDLALYGAQVRARIEPSKTVALTLSAADLYFNGTQFITPVQFFGPNI
                     QFPVNVTIPASGSSPARTVTAQVSVPRDLLVAGNTTLGVSTASNNATNRDGRLSSGYN
                     LVDLIARLDLTRSKRWPAVFLFNYVRNTQAHNVVTAGPG"
BASE COUNT          471 a          960 c          856 g          408 t
ORIGIN      
        1 gtcgtagagc agcgcgagga ttttcgcctt gccgcgcagc gcctcaagct cttcgctgag
       61 ggcggcggca gccgccgccc ggtggctgcc gctggtggtg gtggtggtgc gcgcggccag
      121 cgccgagcgg atcacgtcgt tggccgagac cagcagttgc gtgtggccga gcggcttgtc
      181 gtcgtagcgc acgtccgcgt cactctgctc gggccgcgcc gcgcgctcga acttcaggtg
      241 gctcgcgccg atggtcacgc ggtcgccgtc ggcgagcgga tgctcgaagc gctgctcgcc
      301 gccgacgaag acgtggttgg cgctgcgccc cccgccgacc ttgccatcga cgaggatgta
      361 ggcgccgtcc tgaaacttga cgtgcgcgtg gtagcgcgag gcgcgcgggt cgttcagcac
      421 caccgtgttg tcgggcgcgc gcccgatggt gcacaaaccc tgcggcgtga gcgggcactc
      481 ccactgccga ccgctggcgt ccgtgatgtg cagtagagcc ataacccgaa tccttaacac
      541 ctgtgatgag gaggctttcc gccgggattc tacatgcaac gcggggaaaa agacagaggt
      601 cagaggtcgg aggtcggagg tcagtttcag ttcagaattc aatcacgcac ggcaccggct
      661 cgggttcttc actgacctcc ggcctctgac ccctgacctc cgtttctgta acactccggt
      721 aacgtcggtg cggtaggatg cacgctccca acaaggcggt cacgcttcag ccggaaagtt
      781 ccggagttca tccgcgaagg agtcccccct tcttatgtct atcgcccacg aactgagttg
      841 cgacgttgcg accgccatgc tcgccaaccc cgcggacgaa cagaggggag ccgcgggcgg
      901 cgccctgacc gacatcgtgc tcgaagtcca cacgaccttg cggcgtctca cgaacgaagc
      961 gcggcaaagg cgcgcgcaag ccttccggcc cgaggcgccg ccgaacgccg ccggcaccgc
     1021 cgcctccggc ggctactgac cccgtcatcg aaaacccttc cgcctaagac cgcgcctccc
     1081 gcggcgcgcc gatgagtgcg cccgcccgcg aacgtgaaga gaacccgcgc cgcttgtaac
     1141 ttagagttca gatggccgta atatgtcgtt aagacaacgg acataggatt ctctgtctcc
     1201 acaccgttcg ctcgggaagg atcaacacat gcgtcaactc gctctccgcg cactcctctt
     1261 gtgcacgctc ggctccgcgc tgtcgatggc gaccgccgcg cagcaacagc cgccgccgca
     1321 gaacacggac gccggggcgg tgaaggcggc gccggacgcc gccgacaaaa cgggcgccgc
     1381 cgtcgtggac ggcaacccgc tcggggcgga agccatcgag cgccgtctgc gcgagcagcg
     1441 cgaggagatc gagagcctgc gcgcgctcgt cctggagcag acgcggctcg tcaaagaatt
     1501 gaaggggcgc gtcgagcggg ccgagcagca gcagcggcag gcggcgcaac aaccggctca
     1561 agcggtcttc agagacgcat cctacgcagc ggctctcccg tcaagcaacg ccgggacgac
     1621 tcccgccgag agagagactg cgggcgcggc gcaggacgcg aagctggaag agcgcgtcgc
     1681 gcgcgtcgag aaggcgagcg aggccgtcac caagcagctc gccggcatca ccttcttcgg
     1741 cgacctgcgc ttccgctacg agtccatcta cggccagcag aacgcgctcg ccagcgccga
     1801 cacgccgggc gcgctcggca acccgctcac gccgcgccag cgtctgcgcc tgcgcgcgcg
     1861 cttcggcgcg cgcggcacca tcggcaagga gttcgagtgg ggcatccgtt tcgccaccgg
     1921 ctcgttcgcc gacaccatcg catcgaacca gacgctcacg gacttctaca accgcaagtc
     1981 cttcgcgctc gacaacgcct acctcacgtg gaagccctcg cgggcgcccg gtctacaggt
     2041 gcagggcggc aagttcgaga caccgtgggt gcgcaccgag atgacatggg acaatgacct
     2101 caacgtcgaa ggcttgcacg agagctacac gcgcacgttc gagaagtcga agctgaagag
     2161 attcgacctc atcgcgtggc agctcccgat gctcgaacgc aactcggcct tcgtgctcaa
     2221 cgccgacggc acggttgact tctccgcgtc ggaacgcggg ggccgcgacc tcgcgctcta
     2281 cggcgcgcag gttcgcgcgc gcatcgagcc ttcgaagacg gtggcgctca cgctctcggc
     2341 ggcagacctc tacttcaacg gcacgcagtt catcacgccc gtccagttct tcggcccgaa
     2401 catccagttc cccgtcaacg tcacgatccc cgcgagcggc agcagccctg cgcgcaccgt
     2461 cacggcgcag gtctccgtcc cgcgcgacct gctcgtcgcg ggcaacacca ccctcggcgt
     2521 ctccaccgcc tcgaacaacg ccacgaaccg cgacggtcgt ctctcttccg gctacaacct
     2581 cgtggacttg atcgcgcgcc tcgacctcac gcgcagcaag cgctggcccg ccgtcttcct
     2641 cttcaactac gttcgcaaca cgcaggcgca caatgtcgtc acggcggggc cgggc
//
LOCUS       DBNGOH010000703         1829 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4503252, whole genome shotgun sequence.
ACCESSION   DBNGOH010000703 DBNGOH010000000
VERSION     DBNGOH010000703.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1829)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1829)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1829
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4503252"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            427..1506
                     /locus_tag="AC159P_13645"
     CDS             427..1506
                     /locus_tag="AC159P_13645"
                     /inference="COORDINATES: protein motif:HMM:NF015651.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HIR3696538.1"
                     /translation="MSQEVNRKSTSRRFAPAGIVFAVVGAALFAYFVWRAGPSEIWAN
                     ISKMGAGFALVLVLSGARFAVRAWAWTLCFEAPHRLSVWEAFKAHLIGDTAGNIISLG
                     MVVSEPTKAAMVRERVPLLSAVAALAVENLFYMLSVVVFLFCGAATLLLVFPLTKLLW
                     WLSVGILAGVVVAVSVASWAVRRQLRFLTAALSWANRRGLGRGSFERRRLRAAALEEK
                     VYGFHTRHRARFVPILLLQSSFHLLGVAEAYLTIHLISDVPPTLLTAFLFETVNRIIN
                     IVFKYIPLRAGVDEAGTGALAVVLKFGVNAGVTLAIVRKARMVFWMSIGIILLVSRGL
                     SLRAVAAEAERAQQAVTNESLESGV"
     gene            1616..>1829
                     /locus_tag="AC159P_13650"
     CDS             1616..>1829
                     /locus_tag="AC159P_13650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696539.1"
                     /translation="MARSIELGAEKAGALPLPRERARGRGAADVWERHAVVVLVVLAA
                     VGFSLRAGGLGQVGFAEDEINKLDAVA"
BASE COUNT          245 a          547 c          702 g          335 t
ORIGIN      
        1 cggcctctta gaagcagcgg cgccttcatc atcgtgcggc tgtcaggtgt cgtaattatc
       61 cggcgcgtgg gcagcgcgcg cgggaagatt gagagggcgc gtgcgcccga gtttccttga
      121 agaaagacgc ggcgtgcgcg tgggcgcgcg agttgcttgg gagacgtgca agccgccgcg
      181 gcgccgcgag atcgtgtggc gagagcgtgc gccgagggaa gggggcgcgt gaggacgtgg
      241 ggagactcct cgcgcgtctt ctcccggcgc ctctcgccgc gggtcgagcg acgacgcggc
      301 ggctgtgcgg tatagcacga acgctgtaca cggtttgagg cgggggcggg gatggcgtat
      361 cttgccgagt gtccgcagag ggcggcaaca agggagaggg aagcggtggc cgcccccgcg
      421 tcgtcgatga gtcaagaggt caatcgtaaa agcacgtcgc gcaggttcgc gcccgcaggg
      481 atagtcttcg ccgtcgtggg cgcggcgctg ttcgcgtact tcgtgtggcg cgcggggccg
      541 tcggagattt gggcgaacat ctcgaagatg ggcgcgggct tcgcgctcgt cctcgtgctg
      601 tcgggcgcgc gcttcgcggt gcgcgcgtgg gcgtggacgc tctgcttcga ggcgccgcac
      661 cgcctgtcgg tgtgggaggc tttcaaggcg catctgatcg gcgacacggc gggcaacatc
      721 atctcgctcg gcatggtggt gagcgagccg acgaaggcgg cgatggtgcg cgagcgcgtg
      781 ccgctgctct cggcggtggc ggcgctggcg gtcgagaatt tgttctacat gctctcggtc
      841 gtcgtcttcc tcttctgcgg ggcggcgacg ctgctgctgg tcttcccgct gacgaagctg
      901 ctgtggtggc tgagcgtcgg gatactggcg ggcgtcgtcg tggcggtcag tgtcgcgtcg
      961 tgggccgtca ggcggcagtt gcggttcctg acggcggcgc tctcgtgggc caaccggcgg
     1021 ggcctcgggc gcgggtcgtt cgagcggcgg cgcctgcgcg cggcggcgct cgaagagaag
     1081 gtttacggct tccacacgcg gcaccgcgcg cgcttcgtgc cgatcctgct gctccaaagc
     1141 tcgttccacc tgctcggcgt cgccgaggcg tacctgacca tccacctcat cagcgacgtg
     1201 ccgccgacgc tcctgaccgc cttcctcttc gagacggtca accgcatcat caacatcgtc
     1261 ttcaagtaca tccccttgcg cgcgggcgtg gacgaggcgg gcacgggcgc gctcgccgtc
     1321 gtcctcaagt tcggcgtcaa cgcgggcgtc acgctcgcca tcgtccgcaa ggcgcggatg
     1381 gtcttctgga tgagcatcgg catcatcctg ctcgtcagcc gcggcctctc cctgcgcgcc
     1441 gtcgccgccg aagccgagcg cgcgcagcag gcggtcacga acgagagtct ggagtccgga
     1501 gtctgaaagt tgaagtcgga agtcagaaga cggaagtcag aattaataag gcagcgaccg
     1561 cttcggtttt aattctgact tccggcttct gacttctgac ttccagaggt tcttgttggc
     1621 tcgctctatc gaactcggcg cggagaaggc gggggcgttg ccgctgccgc gggagcgtgc
     1681 gcgggggcgc ggcgcggcgg acgtgtggga gcggcacgcg gtcgtggtgc tggtggtgct
     1741 ggcggcggtg gggttctctc tgcgcgcggg gggcctgggg caggtcgggt tcgcggagga
     1801 cgagatcaac aagctcgacg cggtcgcgt
//
LOCUS       DBNGOH010000704         3081 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1022543, whole genome shotgun sequence.
ACCESSION   DBNGOH010000704 DBNGOH010000000
VERSION     DBNGOH010000704.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3081)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3081)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3081
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1022543"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            148..798
                     /locus_tag="AC159P_13655"
     CDS             148..798
                     /locus_tag="AC159P_13655"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HIR3696540.1"
                     /translation="MLPSTLGQTGSRQNPPATQQDEVLRISTDLVQTDVIVLDKQGRA
                     VKGLTRDRFELLVDGQPQAVSFFEGVEAGSTQEATKLAAARKQDAAPNSAQPPAAPLR
                     TLAGRSFVFFVDDLHLAPEGVSRTRELLNNFVNRMGEDDQALIISPSGKIGFLQQLTD
                     NKTALKLAASRISYQAQAVPSAGRRAMTVYEALAIDRGQRSVLDYRQRAARRDRHA"
     gene            800..1003
                     /locus_tag="AC159P_13660"
     CDS             800..1003
                     /locus_tag="AC159P_13660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696541.1"
                     /translation="MLQGGKKVIGAAPQPLPTAGVTDMARLQYGSEVPLSTLAPGRNT
                     LKVTVNDRVSKASASQQLNFIIE"
     gene            1359..>3081
                     /locus_tag="AC159P_13665"
     CDS             1359..>3081
                     /locus_tag="AC159P_13665"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3696542.1"
                     /translation="MPRRFATALAIALALFAASPSPAHARTAATAQAGNDVVLILPFE
                     NTSGQREFNWIGESFADILSELLGAHGLTVVPGEAREVAYQRLRLPLTVIPSRATAIK
                     LAREAGATLLVLGTYKVTPAADEKEKAEVQGNARVIRVNEGRLSGKVLDGRWASHEFM
                     FGDALVSLQTVQGKLAYQILYEQDDKLPFSQKAIIDLATKVPPRAFEGLVKATMTDDR
                     EKKTNYLKNAMAEYAKANGGAVYTQAAFELGHLYLNQKDYKLADEHFSMLQKKDPHYA
                     EAAFFASLSRWHLGNVQGALDALLPLTSDFQLTSVYNNAGALAAQAARAEQDAAKRES
                     LLKQSTNVLARAVESAPEDQMVRYNYAYALMLSNKYAEAVEHLREVVKQNEREPHALF
                     LFAKALEKSGQAEAAAENDNNARKYFKGYAEAQVEWEKSQTVSRIPVRLRGDIDIASH
                     RPEPPPAPSADTQGLLAKAQGLYAAGRDDEALNEVRDVMRIEPMNPEAYLLTGRIYQR
                     RGEHALAINQLKTSIFWEPKLIDAHILLGRIFLERGDRAQAIAYARNAIQIDPNNQDA
                     IALQRQVQ"
BASE COUNT          582 a         1094 c          954 g          451 t
ORIGIN      
        1 gtttccccgc tccatcgcta aagcacgatg gccctgacga tccatcccac aaccagtccg
       61 cttccagaca gagaaggtga ctcatgtaac ccccctgtca aaatcggtgc ccgtcttttc
      121 ctgtccggcg ctcttctttg ttcttccatg ctgccgtcaa ccctcggtca gacgggaagc
      181 cggcagaacc cgcccgcgac tcagcaggac gaagtgctgc gtatcagcac cgacctggtt
      241 cagacagacg tgatcgttct ggacaagcag ggccgggccg tcaagggcct gaccagagat
      301 cggttcgagt tgttggtgga cgggcagccg caggccgtct ctttcttcga gggcgtcgag
      361 gccgggagca ctcaggaggc gacaaagctc gcggcggcgc gcaagcagga cgcggcgccg
      421 aacagcgccc agcccccagc cgcccccctg cgtactctgg cgggccgctc cttcgtcttc
      481 ttcgtggacg acctgcacct cgccccggag ggcgtgagcc gcacgcgcga gctgctcaat
      541 aatttcgtga acaggatggg cgaagacgac caggcgctca tcatctcgcc cagcggcaag
      601 ataggcttcc tgcaacagtt gaccgacaac aaaacggcgc tcaagctggc cgcctcccgg
      661 atcagctacc aggcccaagc cgtacccagc gccgggcgcc gcgcgatgac cgtgtatgaa
      721 gccctcgcca tcgaccgcgg tcagcgcagc gtgctcgact accggcagcg ggccgcccga
      781 cgcgaccgtc acgcttgagg tgcttcaggg cggcaagaaa gtgatcggcg cggcgccgca
      841 gccgcttccg acggcgggcg tcacggacat ggcacggctc caatacggct cggaggttcc
      901 gctctcgacc cttgcgcccg gacgcaacac gctgaaagtc acggtcaatg accgcgtgtc
      961 gaaagccagc gcgtcgcagc agctcaactt catcatcgaa taaaacggcg gcgccggagg
     1021 cggggtcatt catcccgcgt cgcggcttga aaacggctgc taaagcatcc taaccgaggc
     1081 cgcgggcatc tatgctcatc tgcttgaaaa agcagaggat atgccggctt gtttgagttg
     1141 gtgccagcgg ttacaatacg cctcgaacgg actttcacgg cggcgacacg ggttcgactc
     1201 ccgtacggga cgccacactt cgcggaagac ggagggcgcg cgggcgtcag cgacgctcct
     1261 cgcgtgagag ggttcgagag agaggccgcc gacgccgtac ttgcagttat gggcgccgcc
     1321 ggcctctcgg cttttgacgg cgcacgagga tgctttccat gcccagacga ttcgccaccg
     1381 cgctcgccat cgcgctcgcg ctcttcgccg cttcgccttc gcccgcgcac gcgcgcacag
     1441 ccgcgaccgc gcaggccggc aacgacgtcg tgctcatcct gcccttcgag aacacttcgg
     1501 ggcagaggga gttcaactgg atcggcgaga gcttcgccga catcctctcc gaactcctcg
     1561 gcgcgcacgg cctgacggtc gtgccgggcg aggcgcgcga ggtcgcctac cagcgcctgc
     1621 gcctgccgct caccgtcatc ccctcgcgcg ccaccgccat caagctcgcg cgcgaggcgg
     1681 gtgcgacgct cctcgtgctc ggcacctaca aggtcacgcc cgcggcggac gagaaggaga
     1741 aggccgaggt gcagggcaac gcgcgcgtca tccgcgtcaa cgaggggcgc ctgtcgggca
     1801 aggtgctgga cggtcgctgg gcctcgcacg agttcatgtt cggcgacgcg ctcgtctccc
     1861 tccagaccgt gcagggcaag ctcgcctacc agattctcta cgagcaggac gacaagctgc
     1921 ccttctcgca gaaggccatc atcgatctgg cgaccaaagt tccgccccgc gccttcgagg
     1981 gtctcgtcaa ggcgacgatg accgacgatc gcgagaagaa gaccaactac ctgaagaacg
     2041 cgatggccga gtacgcgaag gcgaacggcg gcgccgtcta cacgcaggcc gccttcgagc
     2101 tgggccacct ctacctcaac cagaaggact acaagctggc cgacgagcac ttctccatgc
     2161 tccagaagaa ggacccgcac tacgccgagg cggccttctt cgcctcgctc agccgctggc
     2221 acctcggcaa cgtgcagggc gcgctcgacg cgctcctgcc gctcacctcc gacttccagt
     2281 tgacgagcgt ctacaacaac gcgggcgccc tcgccgcgca ggccgcgcgc gccgagcagg
     2341 acgccgccaa gcgcgagagc ctgctcaagc aatcgacgaa cgtgctggcg cgcgccgtcg
     2401 agtcggcgcc cgaagaccag atggtacgct acaactacgc ctacgcgctg atgctctcga
     2461 acaagtacgc cgaggccgtc gagcacctgc gcgaggtcgt caagcagaac gagcgcgagc
     2521 cgcacgcgct cttcctcttc gccaaggcgc tggagaagtc cgggcaggcc gaggccgccg
     2581 ccgagaacga caacaacgcg cgcaagtatt tcaagggcta tgccgaggcg caggtcgagt
     2641 gggagaagtc gcagaccgtc tcccgaatcc ccgtgcggct gcgcggcgac atcgacatcg
     2701 cctcgcaccg gcccgagccg ccgcccgccc cttccgccga cacgcaaggg ctgctcgcca
     2761 aagcgcaggg gctgtacgcc gcggggcgcg acgacgaggc gctcaacgag gtgcgcgacg
     2821 tgatgcgcat cgagccgatg aacccggagg cgtacctgct cacgggccgc atctaccagc
     2881 gccgcggcga gcacgcgctc gccatcaacc agttgaagac ctcgatcttc tgggaaccga
     2941 aactgatcga cgcgcacatc ctgctgggcc gcatcttcct cgaacgcggc gaccgcgcgc
     3001 aggccatcgc ctacgcccgc aacgccatcc agatcgaccc caacaatcag gacgccatcg
     3061 ccctccaacg acaggtgcag a
//
LOCUS       DBNGOH010000705         2117 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_5053843, whole genome shotgun sequence.
ACCESSION   DBNGOH010000705 DBNGOH010000000
VERSION     DBNGOH010000705.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2117)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2117)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2117
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_5053843"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1923
                     /locus_tag="AC159P_13670"
     CDS             <1..1923
                     /locus_tag="AC159P_13670"
                     /inference="COORDINATES: protein motif:HMM:NF012371.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HIR3696543.1"
                     /translation="QELRIPALSAAVVKDQRVLWAKGFGFADVENKVAATEHTPYHLA
                     SLTKTFASTVILRLVEEGKIKLDDPVSKYGVAIESEGVVRVRHLFSHTSEGTPGERYR
                     YNGNRFAELDKVVERATGEPFAAHLIRDILDPLGMTETAPNVPPAPPTKPAGAGEAKT
                     EAEVAAAVRSLYAAASAGDVAQMEKLLAPQQNAFGTDGGPPGLAVNTEQMRNAFRGGL
                     KLDLAVHDLSVTPFGDVAVATFVVGGTVKQGDGAPRREGPWRTTHVWHREGGGWKLHH
                     LHQSALTTGLISDKHRQRFETVSKTLAQAYGLDNKFQITKIKYPTYFGTSAGLIASVL
                     DMAKYDIAVDQNKFLKRETQRLAFTPTVSTKGEKLPYGLGWFTQDYKGRALVWHYGYW
                     MGNSSLILKVPDRNLTFIAMANTDNLSRPTDLGSGDVLSSPVGLAFLKTFVLPELFGE
                     PLAEIDWKAAPAGLHAQLKQAAGKPYADVYTKELLVHARKHGSVGELAEAARLYKVYG
                     DLYAKELPDGLGKRQPLAEIKAVANDADKTVEFAVAREQRLRVFATGEGLTGQMYDYG
                     WIENAETGRRVWEMKLAETTHAGGHDKNRKADALVTLPAGKYRLRYKSDDSHSHDNWN
                     SLPPDFDFWGIALWEQ"
BASE COUNT          419 a          738 c          650 g          310 t
ORIGIN      
        1 caggagttgc ggattccggc gctgtccgcc gccgtcgtta aagatcagag agtcctgtgg
       61 gcgaaaggct tcggcttcgc cgacgtggag aacaaggtcg cggccactga gcacacgccc
      121 taccacctcg cgtcgctgac caagacgttc gcctcgaccg tcatcttgcg cctcgtcgaa
      181 gagggcaaga tcaagctcga cgacccggtc tcgaaatacg gcgtggccat cgagagcgag
      241 ggcgtcgtgc gcgtgcgcca tctcttctcg cacacgtccg agggcacgcc cggcgagcgc
      301 taccgctaca acggcaaccg cttcgccgag ctggacaagg tagtcgagcg cgccaccggg
      361 gagcctttcg ccgcgcacct catcagggac atcctcgacc cgctcggcat gacggagacc
      421 gcgcccaacg tcccgcccgc gccgccgacg aaacccgcgg gcgcgggcga agcgaagacc
      481 gaggccgaag tcgccgccgc cgtccgtagt ctctacgccg cggcgagcgc gggcgacgtg
      541 gcgcagatgg aaaagctcct cgcgccgcag cagaacgcct tcgggacgga cggcggcccg
      601 ccgggcctcg ccgtgaacac cgaacaaatg cgcaacgctt ttcgtggtgg cctcaagctc
      661 gacctcgccg tgcacgacct ttcggtcacg cccttcggcg acgtggcggt ggcgaccttc
      721 gtcgtcggcg gcactgtcaa gcaaggcgac ggcgcgccgc gccgcgaagg cccctggcgt
      781 acgacccacg tctggcacag agagggcggc gggtggaagc tccaccactt acatcagtcg
      841 gcgctcacaa cgggcctcat ctccgacaag caccggcagc gcttcgagac cgtctccaaa
      901 acgctggcgc aagcctacgg cctcgacaac aaattccaaa tcacgaagat caagtacccg
      961 acctacttcg gcacgtccgc cggcctcatc gcctccgtgc tcgacatggc taagtacgac
     1021 atcgccgtcg atcagaataa attcctgaag cgcgagacgc agcggctcgc cttcacgccc
     1081 acggtctcga ccaaaggcga gaagctgccc tacggcctcg gctggttcac gcaggattac
     1141 aagggccgcg cgctcgtctg gcactacggc tactggatgg gcaactcctc gctcatcctc
     1201 aaggtgcccg accgtaacct gaccttcatc gcgatggcga acacggacaa cctcagccga
     1261 ccgaccgacc tcggctccgg cgacgtgctg agttcgcccg tcggcctcgc cttcctcaag
     1321 accttcgtcc tgcccgagct tttcggcgag ccgctggccg agatcgactg gaaggcggcg
     1381 cccgccgggc tgcacgcgca gctcaagcag gccgcgggca agccctacgc cgacgtgtac
     1441 acgaaggagc tgctcgtcca cgcgcgcaag cacgggagcg tcggcgagct tgcggaagcc
     1501 gcgcggctct ataaagtcta cggcgacctc tacgcgaagg agctgcccga cggcctcggc
     1561 aagcggcagc cgctcgcgga gatcaaggcg gtcgccaacg acgccgacaa gaccgtcgag
     1621 ttcgccgtcg cgcgcgagca gcgcctccga gtcttcgcca ccggtgaggg gctgaccggg
     1681 cagatgtacg actacggctg gatcgagaac gccgagaccg gccggcgcgt ctgggagatg
     1741 aagctcgccg agaccaccca cgcgggcggc cacgacaaga accgcaaggc ggacgccctc
     1801 gtcacgctcc ccgcgggcaa atacagactg cgctacaagt ccgacgactc gcactcgcac
     1861 gacaactgga acagcctccc gcccgacttc gacttctggg gcatcgccct ctgggagcag
     1921 taggcagcag gcagtaggca gtaggcagtc aagacaagaa gggcgaagat gcttgggagg
     1981 taaagtgaca agcatcttcg ctcgtcaagc gataaagcga cttgccaaat agtaaagcga
     2041 gaggcttgag acgtagcgtc tcaagcctct cgcttattct gactcctgtc ttctgcttta
     2101 actgcctacc gcctact
//
LOCUS       DBNGOH010000706         3025 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3586891, whole genome shotgun sequence.
ACCESSION   DBNGOH010000706 DBNGOH010000000
VERSION     DBNGOH010000706.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3025)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3025)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3025
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3586891"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..372)
                     /locus_tag="AC159P_13675"
     CDS             complement(<1..372)
                     /locus_tag="AC159P_13675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HIR3696544.1"
                     /translation="MWHPEESIRHEIPATRWPTAEEAAGAMLFRPVSVGPVTLENRTW
                     VPAMVPWRATEDGFVTRENLEWYARFAAGRPGAVVVEATGVRDIPSGPLLRIGHDRFI
                     PGLKELVACVRRASSGHTRLLI"
     gene            547..999
                     /locus_tag="AC159P_13680"
     CDS             547..999
                     /locus_tag="AC159P_13680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696545.1"
                     /translation="MSKWENLFGRLGRALLRGGARGHFEQKFVPRPRGDSFAGQAARV
                     AFRLFKDNCVTLPWIEEGAAIDAAVDGARADLLRSWNVYRSSRGSRRGAALAEGDWQQ
                     VLTSFRRHVADLNRRIAAYNLKAPSAKFRRPPVDAEREISRLKEGGKG"
     gene            1268..2467
                     /locus_tag="AC159P_13685"
     CDS             1268..2467
                     /locus_tag="AC159P_13685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HIR3696546.1"
                     /translation="MADDLLEWRREFPILEQTVYLISHSLGAMPRAAAERVQAYTESW
                     ATRGVRAWAEGWWEMPLAVGDEVGRIIGAEPGTVAMHQNVSVCQSLILSCFDTGPETK
                     RNKIVYSELEFPSVMYVYEAHARDGRFRIEKVKSDDGITAPLERMLAAIDEETLLVPL
                     SHVLFKSAFLQDAKAITERAHEVGALVVLDTYQSAGTVPFSVKELGVDFCTGGSVKWL
                     CGGPGAGYLYVAPRLRGELEPKVTGWMAHAAPFAFEDGPIRYAEGAARFLHGSPAVPA
                     LYAAESGYKIINEIGVEAIRAKSTRQTQKLIEMAVEAGFEVTSPRDPARRGGTVTIDA
                     PHAPAVTRELIRRETIVDYRPGAGVRVSPHFYTKDEELEHVVREMRDILDTRAYAAHE
                     EAAGAAF"
     gene            2548..>3025
                     /locus_tag="AC159P_13690"
     CDS             2548..>3025
                     /locus_tag="AC159P_13690"
                     /inference="COORDINATES: protein motif:HMM:NF015272.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004833 - L-tryptophan 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006569 - L-tryptophan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase family protein"
                     /protein_id="HIR3696547.1"
                     /translation="MAKEYGAGAPLSYNKYLRVPDLIALQDCLSDPAHHDELLFITIH
                     QTYELWFKQVLHELDAAVAAMSEDRAPVATRALSRVVEIEKLLVTQIHILETMTPISF
                     LGFRDELNPASGFQSMQFREIEFASGLKDRAVLRAFRDDEFASVRLGARFEAPTLAD"
BASE COUNT          511 a         1046 c          977 g          491 t
ORIGIN      
        1 tattaagagc cgcgtgtgtc cgctgcttgc gcggcgtaca cacgcgacca gctcttttaa
       61 gccggggatg aagcggtcgt gcccgatccg caggagcggc cccgaaggga tgtcgcgcac
      121 gcccgtcgcc tcgaccacga cggcgcccgg tcgccccgcc gcgaagcgcg cgtaccattc
      181 gaggttctcc cgcgtcacga acccgtcctc ggtcgcgcgc cacggcacca tcgcgggcac
      241 ccacgtccgg ttctccaatg tgacagggcc gacgctgacg ggccggaaga gcatggcgcc
      301 tgcggcctct tcagccgtcg gccagcgcgt cgcggggatt tcgtgccgga tggattcttc
      361 ggggtgccac atagcttcag ggaagccgag tgtagcagca accggcgggc gccggaaaat
      421 gcgcgcccgg aaaaagcgga aacacgccgc gcgcgcgggg cgtagagcag agggcaggag
      481 cgcccctcgg attttgagca cgcctccggc tgcccgcggc gccggggccg gaaggtcaag
      541 agaattatga gcaagtggga gaacctgttc ggcagactcg ggcgggcgtt gctgcgcggc
      601 ggcgcgcgcg gccacttcga acaaaagttt gtgccgcgcc cgcgcggcga ctcgttcgcg
      661 ggtcaggccg cgcgcgtcgc gttccgtctc ttcaaagaca actgcgtcac gctcccctgg
      721 atcgaggagg gcgcggccat cgacgccgcc gtggacggcg cccgcgcaga cctcctgcgc
      781 tcgtggaacg tctaccgttc gagccgcggc tcgcggcgcg gcgccgcgct cgccgaaggc
      841 gactggcaac aggtgctgac ctccttccgc cgccacgtcg cagacctcaa ccgccgcatc
      901 gccgcctaca acctcaaggc gccctccgcc aagttccgcc gcccccccgt tgacgccgag
      961 cgcgagataa gcaggctgaa agaagggggg aaagggtaaa aggggaagag gcgaaaagga
     1021 agacgaaaga cagcgcggct ctcatcaagt tgacgatgag agccgcgctg cttcttatcc
     1081 ttgctgcttc ttatccttga tcgaagatga gagccgcgct gcctctcatc ccccctcttc
     1141 gcctcttccc ctcttcccct tttcccctct tcccccttct tccttttcgc cctttccccg
     1201 tttccccttt tccccccaag gttttatact gagtcggcaa acctaccggc gcgaggagac
     1261 ctgacttttg gcggacgacc ttttagagtg gcggcgcgag ttccccatcc ttgagcagac
     1321 cgtttacctg atctcgcaca gcctcggcgc gatgccgcgc gccgcggcgg agcgggtgca
     1381 agcctacacg gagtcgtggg cgacgcgcgg cgtgcgcgcg tgggccgagg ggtggtggga
     1441 gatgccgctc gcggtgggcg acgaggtggg gcgcatcatc ggcgccgagc cgggcacggt
     1501 cgccatgcac cagaacgtct cggtctgcca gtcgctcatc ctctcctgct tcgacaccgg
     1561 ccccgagacg aagcggaata aaatcgtcta ctccgaactc gagttcccct cggtcatgta
     1621 cgtctacgag gcgcacgcgc gcgacgggcg ttttcggatc gagaaggtca aatcggacga
     1681 cgggatcacc gcgccgctcg agcggatgct ggcggcgatt gacgaggaga cgctgctcgt
     1741 cccgctctcg cacgtgctct tcaagagcgc gttccttcag gacgcgaagg ccatcaccga
     1801 gcgcgcgcac gaggtcggcg cgctcgtcgt gctcgacacc tatcagtcgg cgggcaccgt
     1861 cccgttcagc gtgaaggagt tgggcgttga cttctgcacg ggcggctccg tgaagtggct
     1921 ctgcggcggc cccggcgcgg gctacctcta cgtcgcgccg cggctgcgcg gtgaattgga
     1981 gccgaaggtc acgggatgga tggcgcacgc cgcgccgttc gccttcgagg acgggccgat
     2041 ccgctacgcg gagggcgccg cgcgcttcct ccacggctcg cccgccgtcc cggctctgta
     2101 cgccgccgag tccggctaca aaatcatcaa cgagatcggc gtcgaagcca tccgcgccaa
     2161 gagcacgcgg cagacgcaga agttaatcga gatggccgtc gaagcgggct tcgaagtcac
     2221 cagcccgcgc gaccccgcgc gccgcggcgg caccgtcacc atcgacgccc cgcacgcgcc
     2281 cgccgtcacg cgcgagctga tccgccgcga gaccatcgtc gattaccgcc ccggcgcggg
     2341 cgtgcgcgtc tcgccccact tctacacgaa ggacgaggaa ctcgaacacg tcgtccgcga
     2401 gatgcgcgac atcctcgaca cgcgcgccta cgccgcgcac gaggaggcgg cgggcgccgc
     2461 tttttaaagt actgcgtgct gagtgctgag gactgagtaa aaacaactcg tctttactca
     2521 gtactcagca ctcagcactg agaaattatg gctaaagaat acggcgcggg tgcgccgctc
     2581 tcatacaaca agtacctgcg agtgcccgac ctgatcgcgc ttcaggactg cctgagcgat
     2641 ccggcgcacc acgacgagct gctgttcatc acgatccacc agacgtacga gctgtggttc
     2701 aagcaagtgc tgcacgagct ggacgccgcc gttgccgcga tgtcggaaga ccgcgcgccg
     2761 gtggcgacgc gcgccctctc gcgcgtcgtc gagatcgaga agctgctcgt cacgcagatt
     2821 cacatcctcg agacgatgac gccgatcagc ttcctcggct tccgcgacga gctgaacccg
     2881 gcgagcggct tccagtcgat gcagttccgc gagatcgagt tcgcctccgg cctcaaagac
     2941 cgcgcggtgc tgcgcgcctt ccgcgacgac gagttcgcgt ccgtcaggtt gggcgcgcgc
     3001 ttcgaggcgc cgacgctcgc cgacg
//
LOCUS       DBNGOH010000707         2912 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3404901, whole genome shotgun sequence.
ACCESSION   DBNGOH010000707 DBNGOH010000000
VERSION     DBNGOH010000707.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2912)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2912)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2912
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3404901"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..692
                     /locus_tag="AC159P_13695"
     CDS             <1..692
                     /locus_tag="AC159P_13695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696548.1"
                     /translation="SRWAGWKLIFVIFFVLYGVTTVMSQIETAFFVTRLPPGMLPRLF
                     LAGAFIAALYSTLAVLLLGKRRPDRAGDESRTRLAMSAGEWAFKLSLIVIAYVVIYFT
                     FGYFIAWKSAAVRDYYGGSDPGSFIAQMRAVVRETPWLVPLQVARALLWAAIAVPVIR
                     MTRGPLWEAGLAVALVFAEVMNSQLLLPNPLMPPEVRAVHLLETASSNFLFGWLVVLV
                     LRGRRALGAEK"
     gene            complement(793..1980)
                     /locus_tag="AC159P_13700"
     CDS             complement(793..1980)
                     /locus_tag="AC159P_13700"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3696549.1"
                     /translation="MSRRSRLLAACRAALLAFLFAAAPAAGFPRPAQDAAALFKQGAE
                     ALQAGRHREALELFRQVVGVRPDFAEGHWAVGSALLGLGNYEEAMAAFRQALTLKPDL
                     SAAHNGVGSAQMALGRTQEALASFKAALRADPQDAGAHYGAGNALYNLRRFADAAAEY
                     EQTARLAPEAPDAHANLSAAYYNLDRYEEAISAAAEAVRVKPDFAAGYVSLSWYYSMV
                     DRHRESLDSARKGAALDPTNQMAFTNMCRAYNDLSLYSEALDACGKALRLKPDDPETL
                     YYIGVAHKGMKRGAEAARAFARAAPLFGQIQGAEVDPLYLQGAAYTQLGKYTEAVASY
                     RKAIALRPNFVKARYNLGVTCVLASDQGCAREEYESLKALSPSKAAKLAELMAERRPG
                     GKR"
     gene            complement(2429..2686)
                     /locus_tag="AC159P_13705"
     CDS             complement(2429..2686)
                     /locus_tag="AC159P_13705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696550.1"
                     /translation="MSGSEERERRTALIVTTLVKFYATVELSGAACPLCRGASEHDEE
                     CPLSLAWSLLSAKQKGEVRRAMRAYALSVGHAEEWPDTLSQ"
     gene            complement(2742..>2912)
                     /locus_tag="AC159P_13710"
     CDS             complement(2742..>2912)
                     /locus_tag="AC159P_13710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HIR3696551.1"
                     /translation="GGGALLKNLDKRLSIETGLPVSIADDPLSSVVLGAGKMLSDFAL
                     LKRVRWERSMAA"
BASE COUNT          435 a          998 c          940 g          539 t
ORIGIN      
        1 gttctcgctg ggcggggtgg aaacttattt tcgtgatctt tttcgtctta tacggcgtga
       61 caactgtcat gtcgcaaatc gagaccgctt tcttcgtgac acgtctgccg ccaggcatgc
      121 tgccgcgcct cttcctggcc ggggcgttca tcgcggctct ttattccacg ctcgccgtgc
      181 tgctcctcgg gaagagaagg cccgaccggg cgggggacga aagccgcacg cgactggcga
      241 tgtctgccgg cgaatgggcc tttaaactgt ccctgatcgt catcgcctac gtggtcatct
      301 atttcacctt cggctacttc atcgcctgga agagcgcggc ggtgcgtgac tattacggcg
      361 ggagcgatcc gggcagcttc atcgcacaga tgcgggccgt ggtgcgggag acgccgtggc
      421 ttgtgccgct ccaggtggcg cgcgcgctcc tgtgggcggc catcgcggtg ccggtcatcc
      481 gcatgacgag aggacctttg tgggaagcag ggctggcggt cgcgctggtg ttcgcggagg
      541 tgatgaactc ccaactgctg ttacccaacc cgctcatgcc gccagaagtg cgggcggtac
      601 atctgttgga gacggcctcc tccaactttc tgttcggctg gctggtggtg ctggtgttac
      661 gcgggcggcg cgcgctcggg gcggaaaagt agcacggcta atccgcggat ctgccacctc
      721 gaccgtgact taatccgtca actatccttg ggtgtccggg cgctgtacgc atggtcgggc
      781 gccggcgcgt gattagcgct tgccgcccgg cctcctctcg gccatcagct cggcgagttt
      841 cgccgccttc gaaggcgaca gcgctttcag cgactcgtac tcttcgcgcg cgcacccctg
      901 atccgaggcg aggacgcaag tcaccccgag gttgtagcgt gccttgacga agttaggccg
      961 cagcgcgatg gcctttctgt aagacgccac agcctctgtg tacttgccga gctgcgtgta
     1021 agccgccccc tggaggtaga gcgggtccac ctccgcgccc tggatttggc cgaacagcgg
     1081 cgcggcgcgc gcgaacgccc gcgcggcttc cgccccgcgt ttcattcctt tgtgggcgac
     1141 gccgatgtag tagagcgtct cggggtcgtc cggcttcagc ctgagcgcct tgccgcaggc
     1201 gtcgagcgct tcggagtaga gactcaggtc gttgtaggcc cggcacatgt tggtgaaggc
     1261 catctggttc gtggggtcga gcgccgcccc cttacgcgcc gaatcgagcg actcgcggtg
     1321 acgatccacc atgctgtagt accagctcag gctcacgtaa cccgcggcga aatccggttt
     1381 gacccgcacg gcttcggctg ccgccgatat cgcctcctcg tagcggtcaa ggttgtagta
     1441 ggcggcgctc aggttggcgt gagcgtccgg cgcttcgggg gcgagccgcg cggtctgctc
     1501 gtactccgcg gcggcgtccg cgaaccggcg caggttgtag agcgcgttgc cggccccgta
     1561 gtgcgcgccg gcgtcctgcg ggtctgcgcg cagcgcggcc ttgaaggagg ccagcgcctc
     1621 ctgcgtccgc ccgagggcca tctgcgcgct cccgacgccg ttgtgggccg ccgacaaatc
     1681 cggcttcagc gtgagcgctt ggcggaaagc tgccatcgcc tcttcgtaat tgccaaggcc
     1741 gaggagcgcg ctgccgacgg cccagtggcc ctcggcgaag tccggcctga cgccgactac
     1801 ctgccggaag agttccagcg cttcccggtg tcgcccggcc tgcaacgcct cggccccctg
     1861 tttgaaaagg gcggctgcgt cctgcgcggg tcgcgggaag ccagccgccg gcgccgccgc
     1921 aaagagaaac gcgaggagcg ccgcgcgaca ggcggcgagc agccgtgatc tgcgactcat
     1981 attgtttact ccgccggtgc gacccagagg ccagccggca atgggtcagc acagacccgt
     2041 atgaaactcc acaccgtctc gattgcggtg accctaccgc aacgccgccg ggagcttcgg
     2101 tcggcgaatc accgccctgc tcccgattgc gcgcggcccc gtccatccgc catgaaccat
     2161 accctccaaa ccttcgctac gttaaccgac tctcccattg gttaacgcga gaaaaaccgt
     2221 catgcgggct cacgtccgcc gaccgcggct ggctggatgc ggcgtcttct caaggcagcc
     2281 ccggcgagat acttccggcg cttcgactcg aagcttttgg tgacgatgaa atgcgaaagg
     2341 gcacgccgcc cccgtggccg gcgtgccctt tcctgatcgt gcgcggcggg agccgccccg
     2401 ccggtatttc acgcggcgcg cccgcgactt actgcgacag tgtgtcgggc cattcctcgg
     2461 cgtgcccgac cgacagggcg taggcacgca tggccctcct cacttcgccc ttctgctttg
     2521 cactcagcag tgaccaggcg agcgacagcg ggcattcttc gtcatgctcg gacgccccgc
     2581 ggcacagcgg gcaggccgcg ccggacaact cgacggtcgc gtagaacttc accagcgtgg
     2641 tgacgatcag cgcggttcgc cgctccctct cctccgaacc tgacatgctc cgcttcgtct
     2701 cgatcccctc tcgtccgcgg acgcgagggc gcggtaaacg gctacgccgc catcgaacgc
     2761 tcccacctga ccctcttcag gagcgcgaag tcggagagca tcttgcccgc cccgagcacc
     2821 accgacgaga gcgggtcgtc cgcgatcgag accggcagcc ccgtctcgat cgacagccgc
     2881 ttgtccaggt tcttcagcag cgcacccccg cc
//
LOCUS       DBNGOH010000708         3178 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4320364, whole genome shotgun sequence.
ACCESSION   DBNGOH010000708 DBNGOH010000000
VERSION     DBNGOH010000708.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3178)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3178)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3178
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4320364"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(232..2685)
                     /gene="lon"
                     /locus_tag="AC159P_13715"
     CDS             complement(232..2685)
                     /gene="lon"
                     /locus_tag="AC159P_13715"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013706698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HIR3696552.1"
                     /translation="MPSITNITEGFDAAVEAGEEQQLQIPETLPVLPLRDIVIYPFMI
                     VPLFVSREKSIRAVDVALGENRMIFLVSQRDLDKEEPAGEDLYQTGTVAVIMRMLKLP
                     DGRIRILVQGLSRARVESVEDTGEYLKARLSPMQEAASPSAPLEVEALVRNVRGSMEK
                     AANLGKNISPEVMAIVTSLDEPGRLADLAASNLELKVEDAQSVLDIADTTIRLRRVND
                     LLNKEIEVLTVQQEINTQARADIDRSQREFYLRQQMKAIQSELGEGNELAEEIAQFRE
                     KIEAAQMPKHAEEEALRQLKKLERMHPDAAETATLRNWMEIMTDLPWSKASKDNLELD
                     KAEKILNDDHYGLEKVKERIIEALAVRKLKEKPKGTIMCLVGPPGVGKTSLGRSIARA
                     LDRKIVRLSLGGLHDEAEIRGHRRTYVGAMPGRIVQALQQAGTNNPLIMLDEIDKVGA
                     DFRGDPSSALLEVLDPEQNNSFRDNYLGVTFDLSNVMFMTTANVLETVQPALRDRMEV
                     IRLAGYTEEEKLEIARRHLLPKQIEENGIAAKNVQISKKALTSIILQYTQEAGLRQLE
                     REIGKVCRKVARRIAEGRSETVRVSPKNLHEFLGTPKVFPEEILKKDQIGIATGLAWT
                     AVGGDVLFIEALRMRGKGNLVLTGQLGDVMRESAQAAFSYAKSRAKELDIPEEDFDKY
                     DMHIHIPEGAIPKDGPSAGITLATAMVSSLSQRAVRKDVAMTGEITLRGNVLPVGGVK
                     EKVLAARRARVTKIILPQLNRRDMDEVPKELFGEIQFVFVEHVRDVFREALKEKAPPA
                     VTTPRERPPRLPQAAAPLR"
     gene            complement(2769..>3178)
                     /locus_tag="AC159P_13720"
     CDS             complement(2769..>3178)
                     /locus_tag="AC159P_13720"
                     /inference="COORDINATES: protein motif:HMM:NF012241.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HIR3696553.1"
                     /translation="SRVGRLFAALQEASEMFAPMAPGEWVPPVDLRESESEVTVSVEV
                     PGVKAEHLEVALTGERLRVSGRKRKSTPRGAISHLCSERDYGRFTRLVRLHWPVRAAE
                     ATAELKDGLLIVRLPKLKERRGGEIKIQVRNDE"
BASE COUNT          470 a         1069 c         1016 g          623 t
ORIGIN      
        1 tgttccgggg acttttaaga tgccccgcgc tccccgcgcg ttgcgtccgc cgaaggcgat
       61 tttcaaggcc gaaactgccc gcaaccccta ggaaaagcac cgcccgcgcc gcccgccgcc
      121 gtacggcccg gacgacaact ttacacgccc cctggccttc ggggttgcaa aaaagccgag
      181 gacgagaggc acggtgcctt tcgtcctcgg cttttacctt acttttctgc cttacctgag
      241 cggcgcggcg gcctgcggca gacgcggcgg gcgctcgcgg ggcgtcgtga cggcgggcgg
      301 cgccttctcc ttgagcgcct cacggaacac gtcgcggacg tgctcgacga agacgaactg
      361 aatctcgccg aacagctcct tcggcacctc gtccatgtcg cgccggttga gctgcggcag
      421 gatgatcttc gtcacccgcg cgcgccgcgc cgccagcacc ttctccttga cgccgcccac
      481 cgggagcacg ttcccgcgca gggtgatctc gcccgtcatc gccacgtcct tccggacggc
      541 gcgctgcgag agcgaggaga ccatcgccgt cgccagcgtg atgccggcgg aggggccgtc
      601 cttcgggatg gcgccctcgg ggatgtggat gtgcatgtcg tacttgtcga agtcctcttc
      661 ggggatgtcg agttccttgg cgcgcgactt ggcgtaggag aacgcggcct gcgccgactc
      721 gcgcatcacg tcgccgagct ggcccgtcaa aacgaggttg cccttgccgc gcatcctgag
      781 cgcctcgatg aagagcacgt cgccgccgac ggcagtccac gccagccccg tggcgatgcc
      841 gatctgatcc ttcttcagaa tctcttcggg gaagactttg ggcgtgccca agaattcgtg
      901 caggttcttg ggcgagacgc gcacggtctc cgagcgcccc tcggcgatgc ggcgcgcgac
      961 cttgcggcag accttgccga tctcgcgttc gagctgccgg aggcccgcct cctgcgtgta
     1021 ttgcaggatg atcgaggtca gcgccttctt cgagatttgc acgttcttcg cggcgatgcc
     1081 gttctcctcg atctgcttgg gcaggagatg gcggcgcgcg atttccagtt tctcttcctc
     1141 ggtgtagccg gcgaggcgga tgacctccat gcggtcgcgc agcgcgggct ggacggtttc
     1201 gagcacgttg gccgtcgtca tgaacatcac gttcgacaag tcgaacgtga cgccgaggta
     1261 gttgtcgcgg aaagagttgt tctgctccgg gtcgaggact tcgaggagcg cggacgaagg
     1321 gtcgccgcgg aagtctgcgc cgaccttgtc gatctcgtca agcatgatga gcgggttgtt
     1381 cgtgcccgcc tgctgcaaag cctgcacgat gcggcccggc atcgcgccga cgtacgtgcg
     1441 gcggtggccg cgtatctcgg cctcgtcgtg caggccgccc agagagaggc gcacgatctt
     1501 gcggtcgagc gcgcgcgcga tggagcgacc gaggctcgtc ttgccgacgc ccggcgggcc
     1561 gaccagacac atgatcgtgc ccttcggctt ctccttgagc ttgcgcacgg ccagcgcttc
     1621 gatgatgcgc tctttgactt tttcgaggcc gtagtggtcg tcgttcagaa tcttctcggc
     1681 cttgtcgagt tcgaggttgt ccttcgaagc cttcgaccac ggcaggtcgg tcatgatctc
     1741 catccagttc ctgagcgtgg ccgtctccgc cgcgtccgga tgcatgcgtt cgagcttctt
     1801 gagctggcgg agcgcctcct cctcggcgtg cttcggcatc tgcgcggcct cgatcttctc
     1861 gcggaactgc gcgatctctt cggccagctc gttgccctcg ccaagttccg actggatggc
     1921 cttcatctgc tggcgcaggt agaactcgcg ctgcgagcgg tcgatgtcgg cgcgcgcctg
     1981 cgtgttgatc tcctgctgga cggtgaggac ttcgatctcc ttgttgagga ggtcgttgac
     2041 gcgccgcagc cggatggtcg tgtcggcgat gtccaagacc gactgcgcgt cctcgacctt
     2101 cagctcgagg ttcgacgcgg cgaggtcggc gaggcggccc ggctcgtcga ggctcgtcac
     2161 gatggccatc acctcgggcg agatgttctt gccgaggttc gccgccttct ccatcgagcc
     2221 gcgcacgttg cgcaccagcg cctcgacttc gagcggcgcc gaaggcgagg ccgcctcctg
     2281 catcggactc agacgcgctt tcagatactc gcccgtgtcc tcgaccgact cgacgcgcgc
     2341 gcgcgacaag ccctgcacga ggatgcggat gcgcccgtcc ggcagcttca gcatccgcat
     2401 gatgacggcc acggtgccgg tctggtagag gtcttcgccc gccggctcct ccttgtcgag
     2461 gtcgcgctgc gagacgagga agatcatgcg gttctcgccc aaggccacgt ccaccgcgcg
     2521 gatcgatttt tcgcgcgaga cgaacagcgg cacgatcatg aacggataga tgacgatgtc
     2581 gcgcaacggc aggacgggca gcgtctccgg tatctgcagc tgctgctcct cgccggcctc
     2641 gaccgccgcg tcgaacccct cggtgatgtt tgtgattgaa ggcataactt aattaatgat
     2701 gaatgcggat tgcggaatga tgaatgagaa gcacggtgcc tctcatcact caacctgtct
     2761 ttcattcatc attcatcatt ccgcacttgt attttgattt cccctccgcg ccgctccttg
     2821 agtttcggca ggcggacgat gagcaggccg tctttgagtt cggcggtcgc ctcggcggcg
     2881 cgcaccggcc agtggaggcg gacgaggcgc gtgaacctgc cgtaatcgcg ctccgagcag
     2941 aggtgcgaga tggcgccgcg cggcgtgctt ttccgcttcc tgccgctgac gcgcagccgc
     3001 tcgcccgtca gcgcgacttc gaggtgctcg gccttcacgc ccggcacctc caccgagacc
     3061 gtcacctccg actccgactc gcgcaggtca accggcggca cccactcgcc cggcgccatc
     3121 ggcgcgaaca tctccgacgc ctcctgcaac gccgcgaaca gccgccccac gcgcgacc
//
LOCUS       DBNGOH010000709         3712 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_105957, whole genome shotgun sequence.
ACCESSION   DBNGOH010000709 DBNGOH010000000
VERSION     DBNGOH010000709.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3712)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3712)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3712
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_105957"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="AC159P_13725"
     CDS             <1..243
                     /locus_tag="AC159P_13725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015114347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multinuclear nonheme iron-dependent oxidase"
                     /protein_id="HIR3696554.1"
                     /translation="PLERVVQLHFVGGHRHGDVLVDSHSHETPAEVWELMDEVLARAP
                     VKGAVLERDENLPPFAAVAAELRQARAIGRRHGRWS"
     gene            253..864
                     /locus_tag="AC159P_13730"
     CDS             253..864
                     /locus_tag="AC159P_13730"
                     /inference="COORDINATES: protein motif:HMM:NF053029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO6045 C-terminal-like domain-containing
                     protein"
                     /protein_id="HIR3696555.1"
                     /translation="MGLVEIQRTLARLYTDTRLRERFFGDPVGVGRELGLSAAEAREL
                     SQLSARGVDSFAESLRAKRFLEVTKLLPMTNRMIGGEFAAHFRNYAETYSPAGVKKHL
                     GDALAFASYLENKIRHEREHPRWVLDLLRYETARLKAADPARRLVARVFRHDISRLVR
                     SVARREETPAAHPRLTVAVWWRPQRRGTVRYAVLAAPELPRRK"
     gene            968..1762
                     /locus_tag="AC159P_13735"
     CDS             968..1762
                     /locus_tag="AC159P_13735"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HIR3696556.1"
                     /translation="MSTNEKLIVTLEGGVKRITFNRPERRNAIDFEMFERFAAAVGES
                     ATDGSRVIVITGAGDAFSSGLDLSSLSAGDLAALDVAASVREMINPPIMALRELPVPV
                     VARVHGPAAGIGFSYVLAADIRVASEAAQFSQTFVRVGLMPDGGSTFFLPRLVGYARA
                     FELMATGAQLDARQALSLGLVNRVVAVEDLDAAVNDIAARLASAPQPSLARIKAALGK
                     TEAAELAAALDFEADGQAACFRSPDFVEGVTAFMQKRAPVFGKTRS"
     gene            1889..2935
                     /locus_tag="AC159P_13740"
     CDS             1889..2935
                     /locus_tag="AC159P_13740"
                     /inference="COORDINATES: protein motif:HMM:NF025386.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4236 domain-containing protein"
                     /protein_id="HIR3696557.1"
                     /translation="MGFRFSKSIRLGKFIRLNVSRAGIGGSIGVGPFRIGLGPRGTRF
                     TADLPGAGTSYVKQWGGGPEAKGAGGRRGGKKSDARNVEAEMPTPQAPAEIPAPGFFA
                     PGHEKAFVRALNAEREGRTEEALENFLSAAEEEPGAALSAAILLARLEAGKERAIALL
                     EGVVQRDDEFPTPLMQKYLGEARVPVRVTPSVEATVPADALAATLLLVELYQEQGRVD
                     EAIGLLEELEELAKEPALTLSLCELYAERGLWDGVVERAKDVQAADDVTLETRIHYGR
                     AMQEKGLDEAAIAVFADALKKKKNRSPRLLREAAYRRAVSYQNLGKTSQANREFQKIY
                     AEDPDFRDVAQRVS"
     gene            3149..3664
                     /gene="def"
                     /locus_tag="AC159P_13745"
     CDS             3149..3664
                     /gene="def"
                     /locus_tag="AC159P_13745"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HIR3696558.1"
                     /translation="MALLEIVKYGDPVLETPGEVVTEFDGRLERLVADMFETMYAARG
                     VGLAAPQVCVSKRLFVMDCSGGQDPRARVALVNPSVLRVEGDQTGDEGCLSFPGIFFA
                     VKRSLRAVVRAQDVRGEEFEFDGLELEARCMLHETDHCDGIVFIDRTTTLKRELVKRK
                     IRKLEKQGRWV"
BASE COUNT          638 a         1208 c         1264 g          602 t
ORIGIN      
        1 cctttggagc gggtcgttca gttacatttc gtcggcggcc accgccacgg cgacgtgctc
       61 gtggacagcc actcgcacga gacgcccgcg gaggtctggg agttgatgga cgaagtgctg
      121 gcacgcgcgc cggtgaaggg cgcggtgctc gaacgcgacg agaacctgcc gccgttcgcg
      181 gcggtggcgg cggaactccg gcaggcgcgc gccatcgggc gccggcacgg gcgctggtct
      241 tagtcgagac gcatgggctt agtcgagata cagcggacgc tggcgcggct ctacacggac
      301 acacgcttgc gcgagcgttt cttcggcgac ccggtcggcg tggggcgcga gctgggcctg
      361 agcgccgcgg aggcgcgcga gctgtcgcag ctctctgcgc gcggggtcga ttccttcgcc
      421 gagtctctgc gcgccaagcg cttcctcgaa gtgaccaagc tcctcccgat gaccaaccga
      481 atgatcggcg gcgagttcgc ggcccacttc aggaactacg cggagaccta ctcgcccgcc
      541 ggcgtcaaga aacacctcgg cgacgcgctc gccttcgcct cttaccttga gaacaagatt
      601 cgtcacgaac gcgagcatcc gcgctgggtg ctcgacctgc tgcgctacga gacggcgcgg
      661 ctcaaggccg cagaccccgc gcgccgcctc gtcgcgcgcg tcttccgcca cgacatcagc
      721 cgcctcgtcc gcagcgtcgc gcgccgcgaa gagacgcccg ccgcgcaccc gcgcctgacc
      781 gtcgccgtct ggtggcggcc ccagcgcaga ggcaccgtcc gctacgccgt gctcgccgcg
      841 ccggaactgc cgcggaggaa gtaagcagtg agcagtaaaa acagaaacta cagacgagcg
      901 ttgagcattc atctttaact gctcactgct caccgctcac tgctgactat tcttaggaga
      961 gttcaacgtg tccacgaatg aaaaactgat cgtcacgctg gagggcggtg tcaagcgcat
     1021 caccttcaac cgaccggagc ggcgcaacgc gatcgacttc gagatgttcg agcgcttcgc
     1081 cgccgcggtc ggagagtcgg cgacggacgg cagccgcgtc atcgtcatca cgggcgcggg
     1141 cgacgcattc tcgtccggcc tcgacctgtc gagtctgagt gcgggcgacc tcgccgcgct
     1201 ggatgtcgcg gcgtccgtgc gcgagatgat taacccgccg atcatggcgc tgcgcgagtt
     1261 gcccgtgccg gtcgtcgcgc gcgtccacgg ccccgccgcc ggcatcggct tcagctacgt
     1321 gctcgccgcg gacatccgcg tcgcctccga ggcggcgcag ttcagccaga ccttcgtccg
     1381 cgtcggcctg atgcccgacg gcgggagcac cttcttcctg ccgcgcctcg tcggctacgc
     1441 gcgcgccttc gagctgatgg ccaccggcgc gcaactcgac gcgcgccaag ctctctcgct
     1501 cggactcgtc aaccgcgtcg tcgccgtcga agacctcgac gccgccgtca acgacatcgc
     1561 cgcgcgcctc gcctccgcgc cccagccctc gctcgcacgc atcaaggccg cgctcggtaa
     1621 aaccgaagcc gccgaactcg ccgccgccct cgacttcgaa gccgacggac aggccgcctg
     1681 cttccgctcc cccgacttcg tggagggcgt taccgccttc atgcaaaaac gcgcgccggt
     1741 cttcggcaag acaagaagct agaggctaga ggctggaggc ttgttcgaaa tcatcgcctg
     1801 cgaaacgtga actgtactct tcaagcttga catcacagac gttttcacaa gcctccagtc
     1861 tttaggctct aacctccgct ctaaagttat gggattccgt ttcagcaaaa gcatcaggct
     1921 cggcaagttc atccgtttga acgtgagccg tgcggggata ggcgggagta tcggtgtggg
     1981 gccgttccgc atcggcttgg ggccgcgggg gacgcgtttc acggcggact tgccgggcgc
     2041 ggggacgagc tatgtgaagc agtggggcgg cgggccggag gcgaagggcg cgggcggacg
     2101 aaggggcggg aagaagagtg acgcgcggaa cgtcgaagcg gagatgccga cgccgcaagc
     2161 ccctgccgag attcctgcgc cgggtttctt cgcgccgggg cacgagaagg ctttcgtccg
     2221 cgctttgaac gccgagcgcg aggggcggac ggaggaggcg ctggaaaact tcctctccgc
     2281 cgccgaagaa gagccgggcg cggcactctc cgccgccatc ctgttggctc ggctcgaagc
     2341 cgggaaggag cgtgccattg cgctgctcga aggggtcgtg cagagggacg acgagttccc
     2401 gacgccgctg atgcaaaagt acctcggcga ggcgcgcgtg cccgtccgcg tcacgccgag
     2461 cgtcgaggcg acagtcccgg cggacgcgct cgcggccacg ctgctcctcg tcgagctgta
     2521 tcaggagcag ggccgcgtgg acgaagccat cggcctgctg gaagagctgg aagagctggc
     2581 gaaggagcct gcgctgacgc tctcgctctg cgaactttac gccgagcggg gcttgtggga
     2641 cggcgtcgtc gagcgcgcga aggacgtgca ggccgcggac gacgtgacgc tcgaaaccag
     2701 aatccattac ggtcgcgcga tgcaggagaa gggactcgac gaggcggcaa tcgcggtgtt
     2761 cgccgacgcc ctcaagaaga aaaagaaccg cagcccgcgc ctgctccgcg aagccgccta
     2821 ccgccgcgcc gtctcctacc agaacctcgg caagaccagt caggccaacc gcgagtttca
     2881 gaaaatctac gccgaagacc ccgacttccg cgatgtcgcg cagcgcgttt catgacagta
     2941 ggagcagggc ttgtccctgc ccgtgagtta caggttcgtt caacgtcgaa tccggcgaga
     3001 gggtgcgggc cgcggacttg ctctgcccct acggttattc cgaccgcgtt acgtttactg
     3061 gtacgcgggc agtaattggc gtaaaattag catcagaact cttcctcacg ttcgcttgtc
     3121 cgagtcggca ggtaagggaa ttcaaagaat ggcattgctg gaaatcgtga agtacggcga
     3181 cccggtgttg gagacgccgg gcgaggtggt gacggagttc gacgggcgcc ttgagcggct
     3241 cgtcgcggac atgttcgaga cgatgtacgc ggcgcggggc gtcggtttgg cggcgccgca
     3301 ggtctgcgtg tcgaagcgcc tgttcgtgat ggactgctcg ggcgggcaag acccgcgcgc
     3361 gcgcgtggcg ctggtgaacc cgtcggtgct gcgcgtcgag ggcgaccaga cgggcgacga
     3421 ggggtgtctt tcgttccccg gcatcttctt cgcggtgaag cgcagtctgc gcgcggtcgt
     3481 acgcgcgcag gacgtgcgcg gcgaggagtt cgagttcgac ggcctcgaac tcgaagcgcg
     3541 ctgcatgctg cacgagaccg accactgcga cggcatcgtc ttcatcgacc gcacgaccac
     3601 gctcaagcgc gagctggtga agcgcaagat caggaagctg gagaagcaag ggcgctgggt
     3661 ttaaagcagg cgcgcggtgg tttcccgcag agcgcggtcg cggcttcgtc ga
//
LOCUS       DBNGOH010000710         8193 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3588028, whole genome shotgun sequence.
ACCESSION   DBNGOH010000710 DBNGOH010000000
VERSION     DBNGOH010000710.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8193)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8193)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8193
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3588028"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            90..563
                     /locus_tag="AC159P_13750"
     CDS             90..563
                     /locus_tag="AC159P_13750"
                     /inference="COORDINATES: protein motif:HMM:NF024273.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HIR3696559.1"
                     /translation="MNHDERQALIAQYAAGYDAVRQSLEGFPAERLGEHPLPGKWSAR
                     EIVQHLADSEMNSAIRLRKLLTEDRPTIQGYDQELYAARLDYNARDIAPALDALRGAR
                     ATTAQLLASMTDDDWAREGTHTESGRYTAEDWLRIYAAHAHNHAAQIRRLREEME"
     gene            744..2189
                     /locus_tag="AC159P_13755"
     CDS             744..2189
                     /locus_tag="AC159P_13755"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF010568.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HIR3696560.1"
                     /translation="MTGTQPVSETVIPDLKLLRRGKVRDVYEVDEDHLLVVATDRISA
                     FDCILPTQIARKGEVLTALSRFWFERLSRITPHHLVTTAVAAMPESLHQHEDLLRGRS
                     MFVRRTEVFPVECVVRGYLSGSGWKDYQRTGAVCGHRLPAGLTESAKLAEPLFTPATK
                     AEEGHDENISEARMAEIVGAEVTGRLRDTALALYREAEAYARSRGIIIADTKFEFGRD
                     RDGRILLIDEALTPDSSRFWPADRYQPGRPQASFDKQFVRDYLETLDWDKRPPAPPLP
                     PDVAAATTERYLEAFRLLTGKSLESGLFKMTFFDVFVLALVGASVVAGALRGFVRAVI
                     VGVALVVGLVVAARGYEAAGALLGNLGLVEAGGAANAGGFLLIVCAALAAGFAAGSLA
                     RAGLRQARLEWFDRALGAGFGLVRGVAVCSAFYLALTAFPVRLESVAEARTAPLLAEG
                     ARVLALCTSAEMRGRFLAEYERLFASTLLSS"
     gene            2236..2493
                     /locus_tag="AC159P_13760"
     CDS             2236..2493
                     /locus_tag="AC159P_13760"
                     /inference="COORDINATES: protein motif:HMM:NF014950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR3696561.1"
                     /translation="MQIRPRLEALIDEMLDGQIMLDEAMAEFEKLYIQKALARNKEHL
                     SRTAASLGIHRNTLSKRVSNYRTQERAARTTPRKRPARRTR"
     gene            2737..3066
                     /gene="groES"
                     /locus_tag="AC159P_13765"
     CDS             2737..3066
                     /gene="groES"
                     /locus_tag="AC159P_13765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013420430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HIR3696562.1"
                     /translation="MATNIRPLHDRVIVRRIEEGEQVRGGIIIPDTAKEKPQEGEVVA
                     VGDGKRKDDGTRQELDVKAGDRVLFGKYSGSEIKIDGEELLIMREDEILGVIERAGAA
                     SGKKGGK"
     gene            3135..4778
                     /gene="groL"
                     /locus_tag="AC159P_13770"
     CDS             3135..4778
                     /gene="groL"
                     /locus_tag="AC159P_13770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895894.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HIR3696563.1"
                     /translation="MAKQVIHGEESRAAILRGVNQLADAVKITLGPKGRNVVLDKKFG
                     SPTITKDGVTVAKEIELKDSLENMGAQMVREVASKTSDVAGDGTTTATVLAQAIFREG
                     VKTVAAGANPMALKRGIDKAVEKAVSEIQRMAKPVKGDAIAQVGTISANGDRTIGELI
                     AEAMSKVGKDGVITVEESRTMNTELEVVEGLQFDRGYLSPYFVTDPERMEAVLDNALL
                     LIHEKKISSMKDLLPLLEQIAKMGKPLVIIAEDVEGEALATLVVNKLRGTLNVAAVKA
                     PGFGDRRKAMLEDIAILTGGKVISEDLGIKLENVKIEDLGRAKKVTIDKDNTTVVEGA
                     GKPSDIEGRVKTIKAQIEDTTSDYDREKLQERLAKLVGGVAVIKVGAATETELKEKKA
                     RVEDAMHATRAAVEEGIVPGGGVALVRAAKVLEKFLANAEGDGDPDEQIGVNIVRRAL
                     EEPLRQIVQNSGKEGAVIVEKIRENKKESYGFNAATEQFEDLVESGVIDPAKVTRTAL
                     QNASSIAGLMLTTEALVSELPEDDKTPAMPGGMGGGMGM"
     gene            complement(4859..5401)
                     /locus_tag="AC159P_13775"
     CDS             complement(4859..5401)
                     /locus_tag="AC159P_13775"
                     /inference="COORDINATES: protein motif:HMM:NF014038.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class IV adenylate cyclase"
                     /protein_id="HIR3696564.1"
                     /translation="MGVEIEKKYRLPREREEELRRRLVRLAAAERGREVFEENTIYDG
                     PGLDPHRRVLRLRRAGGRAILTFKERYHSESAIKHQREDETEVSDAAALAAILETLGY
                     RRALVYEKRRSTWTVAGAEVVLDELPFGLFMEIEGTESEIVEAERLLGLEDAEAEHAP
                     YPELTARHGREADGVVEARF"
     gene            complement(5568..6911)
                     /locus_tag="AC159P_13780"
     CDS             complement(5568..6911)
                     /locus_tag="AC159P_13780"
                     /inference="COORDINATES: protein motif:HMM:NF024091.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3696565.1"
                     /translation="MRKTLAVVRREYVQGVRARAFLISTLLAPVFMLVLFLLPGLLFT
                     MKTGGATRLAVVDQEGRMYERVRESILRGEGDDPRERETAPAVGVGTSERQMQSAAEM
                     QTRFEVERAPADGPIEEVKKRLSERVLREELDAYLVLPADILARGEAEFYARNTGDVF
                     TIAVLENRVGRAVIEQRMRDESIDQRRVEELSREVRMETARVTAGGEQRETGGGGFFL
                     AVGVGSFILIAILMYGQAVLSAVVEEKTTRIVEILFSSVRAFPLMAGKLVGVSLVAMT
                     QFAVWALMFLALALYGAAAAAMWGMELTPPAIQPLHLVYALLFFALGFYVYATIYAVI
                     GAVVTTEKEASQIILPVSMLPVVGIYLAFPVIRSPNSAFSFWVSMVPLFSPITMLVRV
                     VTETPPFWQIALSLAIGFATVVGMVWLAARVYRTGMLMYGKRATIPEILRWLRQP"
     gene            complement(6932..7858)
                     /locus_tag="AC159P_13785"
     CDS             complement(6932..7858)
                     /locus_tag="AC159P_13785"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3696566.1"
                     /translation="MKPAADENAVTLRLERVTKRFDDFTAVGDLSLRVRAGRIFGLIG
                     PNGAGKTTTIRMIVGITAPDEGTIELFGEPVSAQTQERIGYLPEERGLYAKMKVGEQL
                     RFFGELKSLDRRRADEAIDRWLDKVKLADWKQKKASELSKGMQQKVQFVAAVLHDPDL
                     VILDEPFSGLDPVSAELLKGIVLELKSAGKTVIFSTHQMSVAEEVCDDICLIDRSRKV
                     LDGSLREVKRGFGRHAVALRAEGHGGVLEDESLVAGVVARGDFEEVLLADGADPQELL
                     RRLVARGAVVSKFERVEPSLHDIFIAQVGGGR"
     gene            8032..>8193
                     /locus_tag="AC159P_13790"
     CDS             8032..>8193
                     /locus_tag="AC159P_13790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020532706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HIR3696567.1"
                     /translation="MTKPQPSEQRDAAQADRLVSLDAFRGLTIAGMILVNNPGTWGAI
                     YDPLEHAAWH"
BASE COUNT         1490 a         2728 c         2690 g         1285 t
ORIGIN      
        1 aggcggaggt cggaggtcgg cattcagtga aggcaggttg ttttcactga ccactgacca
       61 ccggccactg accactgcta aggagcgtta tgaatcacga cgagagacaa gcactcatcg
      121 cacagtacgc ggcgggctac gacgcggtgc gccagagcct cgaaggcttc cccgccgaaa
      181 ggctcggcga gcacccgctg cccggcaagt ggagcgcgcg cgagatcgtc cagcacctcg
      241 ccgacagcga gatgaacagt gccatccgcc tccgcaaact cctgaccgaa gaccgcccga
      301 cgattcaagg ctacgaccag gagctttacg ccgcgcgcct cgactacaac gcacgcgaca
      361 tcgcgcccgc cctcgacgcc ctgcgcggcg cgcgcgccac caccgcccaa ctcctcgcct
      421 ccatgaccga cgacgactgg gcgcgcgaag gcacgcacac cgaaagcggt cgctacaccg
      481 ccgaagactg gctccgcatc tacgccgccc acgcccacaa ccacgccgca cagattcgcc
      541 gcctccgcga agaaatggaa tagccgtgag ccgcgaaccg taaaccgtga taagagaaga
      601 acggtttttt cttatcacgg tttacggttc gcggttcacg gcttttaatt cttgacaagc
      661 atcgcggagc gcgagcacaa tcgcggctcg ccgcttggca agcgttcttc ccaagtcagc
      721 cccaaccgcg actcgtgacc gtgatgaccg gcacacagcc cgtaagcgaa actgttatcc
      781 ccgacctgaa actactcagg cgcggcaagg tgcgcgacgt gtacgaggtg gacgaagacc
      841 acctgctcgt cgtcgccacc gaccgcatct cggccttcga ctgcatcctc ccgacgcaga
      901 tcgcgcgcaa aggcgaagtg ctcaccgcgc tctcgcgctt ctggttcgag cgcctctcgc
      961 gcatcacgcc gcaccacctc gtcacgaccg ctgtcgccgc gatgcccgag tcgcttcatc
     1021 aacacgaaga cctgctgcgt ggccgctcga tgttcgtcag gcgcacggag gtcttccccg
     1081 tcgagtgcgt cgtgcgcggc tacctctcgg gttcgggctg gaaagattac cagcgcacgg
     1141 gcgcggtctg tggccaccgg ctccccgcgg gcctgaccga atccgcgaag ctcgccgagc
     1201 cgctcttcac acccgcgacg aaggccgaag aagggcacga cgagaacatc agcgaggcgc
     1261 gcatggccga gatcgtcggc gccgaggtga cgggcagact gcgcgacacg gccctcgcgc
     1321 tctaccgcga ggccgaagcg tacgcgcgca gccgcggcat catcatcgcc gacaccaagt
     1381 tcgagttcgg gcgtgaccgc gacggacgaa tcctcctgat cgacgaagcg ctcacgcccg
     1441 actcctcgcg cttctggccc gccgaccgct accagccggg gcgcccgcaa gcctcgttcg
     1501 acaagcagtt cgtgcgcgac tacctcgaaa ccctcgactg ggacaagagg ccgcccgcgc
     1561 ccccgctccc ccccgacgtg gccgccgcca ccacggagcg ttacctcgaa gcctttcgcc
     1621 tgctcacggg gaagagtctg gagtccggac tttttaagat gacattcttc gacgtgttcg
     1681 tgctcgcgtt ggtcggggcg tcggtcgtgg ctggcgcgct gcgcgggttc gtgcgggcgg
     1741 tgattgtggg agtcgcgctc gtcgtcggcc tcgtcgtggc ggcgcgtggt tacgaggcgg
     1801 cgggcgcgct gctcggcaac ctcggcctcg tcgaagcggg cggcgcggcg aacgccgggg
     1861 gcttcctctt gatcgtgtgc gcggcgctcg cggcgggctt cgcggcgggg agtctggcgc
     1921 gcgcggggct aaggcaggcg cggctcgaat ggttcgaccg cgcgctcggc gcagggttcg
     1981 gcctcgtccg cggcgtcgcg gtctgctcgg ccttctacct cgcgctgacg gccttccccg
     2041 tgcgcctcga atcggtcgcc gaagcgcgca ccgcgccgct gctcgcggaa ggggcgcggg
     2101 tgctcgcgct ctgcacctcc gccgagatgc gcgggcgctt cctggcggag tacgaacgcc
     2161 tcttcgcttc cactttgcta tcgagttgag atcgccgtgc gattttacag tctagccttt
     2221 gcgacggagt cacccatgca gatacgccca cgcctcgaag cgctgattga cgagatgctc
     2281 gacggacaga tcatgcttga cgaggcgatg gccgagttcg aaaagctcta catccagaag
     2341 gcgctggcgc gcaacaaaga acacctctcg cgcaccgccg cctcgctcgg catccaccgc
     2401 aacacgctct ccaagcgcgt ctccaactac cgcacgcagg aacgcgccgc gcgcaccacg
     2461 ccccgcaaac gccccgcccg ccgcacacgc tagccgcccc gcctcgcccg ccgcacacgc
     2521 cccgcaatcc agccggttga caagccctcg cgggttgcta tatttttagc actccgcaaa
     2581 aaggagtgcc agaaattgac gtttttgcgt ggaaaatgaa gggtattttg cgccttgttt
     2641 ggtggcacgc ccggcgcgag agtgctaacg cgagcttttt gcaatcaaaa gaaactcacc
     2701 gacctacccg taggtgaaca cccgaaagga gcgattatgg caacgaacat cagaccgctg
     2761 catgaccgtg tgatcgtgcg ccgcattgaa gagggcgagc aggtgcgcgg cgggatcatc
     2821 atccccgaca ccgcgaagga gaagccgcag gagggcgagg tcgtcgccgt cggcgatggc
     2881 aagcgcaagg acgacggcac gcgccaggaa ctcgacgtca aggccggcga ccgcgtgctg
     2941 ttcggcaagt acagcggctc ggagatcaag atcgacggcg aggaactgct catcatgcgc
     3001 gaggacgaaa tcctcggcgt catcgagcgc gccggtgcgg ccagcggcaa gaagggcggc
     3061 aagtagtccg acggactcct ggccgctttt tctttgagag attcacaaac gtagttgaag
     3121 gagaagagac tgttatggca aagcaagtga ttcacggtga ggagtcgcgc gcggcgatcc
     3181 tgcgcggcgt caaccagctc gccgacgcgg tgaagatcac gctcggcccg aaggggcgca
     3241 acgtcgtcct cgacaagaag ttcggctcgc cgacgatcac caaggacggc gtcacggtgg
     3301 cgaaggagat cgagctgaag gactcgctcg aaaacatggg cgcgcagatg gtgcgcgaag
     3361 tcgcctcgaa gacttccgat gtcgccggtg acggcaccac cacggcgacc gtgctcgcgc
     3421 aggccatctt ccgcgagggc gtgaagacgg tcgcggcggg cgcgaacccg atggccctga
     3481 agcgcggcat cgacaaagcc gtcgagaagg ccgtctcgga gattcagcgc atggccaagc
     3541 ccgtgaaggg cgacgccatc gcgcaggtcg gcaccatctc ggccaacggc gaccgcacca
     3601 tcggcgagct gatcgccgag gcgatgtcga aggtcggcaa ggacggcgtc atcacggtcg
     3661 aagagtcgcg gacgatgaac accgagcttg aggtcgtcga gggcctccag ttcgaccgcg
     3721 gttacctgtc gccctacttc gtgaccgacc ccgagcggat ggaggcggtg ctcgacaacg
     3781 cgctgctgct catccacgag aagaagatca gctcgatgaa agacctgctc ccgctgctcg
     3841 aacagatcgc gaagatgggc aagccgcttg tcatcatcgc ggaggatgtc gagggcgagg
     3901 cgctggcgac tttggtcgtc aacaagctgc gcggcacctt gaacgtcgcg gcggtgaagg
     3961 cgccgggctt cggcgaccgc cgcaaggcga tgctcgaaga catcgccatc ctgaccggcg
     4021 gcaaggtcat ctcggaagac ctcggcatca agctggagaa cgtcaagatc gaagacctcg
     4081 ggcgcgccaa gaaggtcacg atcgacaagg acaacaccac ggtcgtcgag ggcgcgggca
     4141 agccttcgga catcgagggc cgcgtcaaga ccatcaaggc gcagatcgag gacaccacgt
     4201 ccgactacga ccgcgagaag ttgcaggagc ggctggcgaa gctcgtcggc ggcgtcgcgg
     4261 tcatcaaagt cggcgcggcg accgagaccg agctgaagga gaagaaggcg cgcgtcgagg
     4321 acgcgatgca cgccacgcgc gcggcggtcg aggaaggcat cgtgccgggc ggcggcgtcg
     4381 cgctggtgcg cgcggcgaag gtgctcgaaa agttcctcgc gaacgcggag ggcgacggcg
     4441 acccggacga gcagatcggc gtcaacatcg tccgccgcgc gctcgaagag ccgctgcgcc
     4501 agatcgtgca gaactcgggc aaagagggcg cggtcatcgt cgagaagatt cgtgagaaca
     4561 agaaggagtc ttacggcttc aacgcggcga ccgagcagtt cgaagacctc gtcgagtcgg
     4621 gcgtcatcga cccggcgaag gtcacgcgca cggccttgca gaacgcgtcc tccatcgccg
     4681 gcctgatgct gacgaccgag gcgctggtct ccgagcttcc cgaggacgac aagaccccgg
     4741 cgatgcccgg cggcatgggc ggcggcatgg gcatgtaagc ggcccgcgcc cgcgcaagat
     4801 aaatcggcca cgcttcacac agaagcgtgg ccgattttct tttcgcctac tctccttttc
     4861 aaaagcgagc ttcgacgacg ccgtccgctt ccctgccgtg ccgcgcggtg agttccgggt
     4921 agggcgcgtg ctcggcctcg gcgtcttcga gtccgagcag ccgttcggcc tcgacaatct
     4981 cactttccgt gccctcgatc tccatgaaca ggccgaacgg cagctcgtcg aggacgacct
     5041 ccgcgcccgc gaccgtccag gtgctgcgcc gcttctcgta gacgagcgcg cgcctgtagc
     5101 cgagcgtctc cagtatcgcg gccagcgccg cggcgtcgct cacctcggtc tcgtcctcgc
     5161 gctggtgctt gatggcggac tcggagtggt agcgctcctt gaacgtcagt atcgcgcgcc
     5221 cgcccgctcg ccgcagccgc agcacgcgcc ggtgcgggtc gagtccgggg ccgtcgtaaa
     5281 tcgtgttctc ctcgaacacc tcgcggcccc gctccgccgc cgcaagcctg acgaggcgcc
     5341 gcctcagctc ttcctcgcgc tcacgcggga ggcgatattt cttctcgatc tctacgccca
     5401 taaagaagca ctcagcgatt agctgttagc gattagctgt tagcgttcag cattcaagca
     5461 tgtagtctaa acgaagatcg atttaatgca accgccgccc ttccgcccga agtctgccgc
     5521 gggccatttg ttaatgctaa aagctaacag ctaatcgcta atcgctctca cggctgacga
     5581 agccagcgga ggatttcggg gatggtcgcg cgcttgccgt acatgagcat gccggtgcgg
     5641 tagacgcgcg cggcgagcca gaccatgccg acgaccgtgg cgaagccgat ggcgagcgag
     5701 agggcgatct gccagaaggg gggcgtctcg gtgacgacgc ggacgagcat cgtgatgggc
     5761 gagaagaggg gcaccatcga gacccagaag gagaaggcgg agttggggct gcggatgacg
     5821 gggaacgcga ggtagatgcc gacgacgggc agcatgctga cggggaggat gatctggctc
     5881 gcctccttct cggtggtgac gacggcgccg atgacggcgt agatcgtggc gtagacgtag
     5941 aagccgagcg cgaagaagag gagcgcgtac acgaggtgca gcggctgaat cgcggggggc
     6001 gtcagctcca tcccccacat cgccgccgcc gccgcgccgt agagcgcgag cgcgaggaac
     6061 atgagcgccc agacggcgaa ctgcgtcatc gcgacgagcg agacgccgac gagcttgccg
     6121 gccatgagcg ggaaggcgcg cacggaggag aacagaatct ccacgatgcg cgtcgtcttc
     6181 tcttcgacga ccgcggagag gacggcctgc ccgtacatga ggatggcgat gaggatgaac
     6241 gagccgacgc cgacggcgag gaagaagccg ccgccgcccg tctcgcgctg ctcgccgccc
     6301 gcggtgacgc gcgcggtctc catcctgacc tcgcgcgaca gctcctcgac gcggcgctgg
     6361 tcgatgctct cgtcgcgcat ccgctgctcg atgacggcgc ggcccacgcg attctcaagc
     6421 acggcgatgg tgaacacgtc gcccgtgttg cgcgcgtaga actcggcctc gccgcgcgcg
     6481 aggatgtcgg cgggcaggac gaggtaggcg tccagctctt cgcgcagcac gcgctcgctg
     6541 aggcgcttct tcacctcttc gatggggccg tcggcgggcg cccgctcgac ctcgaagcgc
     6601 gtctgcatct cggcggcgga ctgcatctgc cgctcgctgg tgcccacgcc gacggcgggc
     6661 gcggtctctc tctctcgcgg gtcgtcgcct tcgccgcgga ggatggactc gcgcacgcgc
     6721 tcgtacatcc tgccctcctg atcgacgacg gcgagccgtg tcgcgccgcc cgtcttcatc
     6781 gtgaagagca ggccgggcag gaggaagagg acgagcatga agacgggcgc gagcagcgtc
     6841 gagatgagaa aggcgcgcgc gcgcacgccc tgcacgtact cgcgcctgac gacggccagc
     6901 gtctttctca acgtccctcc cgcgcccttt ctcatcgccc ccccccgacc tgcgcgatga
     6961 agatgtcgtg caggctcggc tcgacgcgct cgaacttcga gacgacggcg ccgcgcgcga
     7021 ccagccggcg gagcagctcc tgcgggtccg cgccgtcggc gagcagcacc tcttcgaagt
     7081 cgccgcgcgc gacgaccccg gccacgagac tttcgtcttc gagcacgccg ccgtgcccct
     7141 ctgcgcgcag cgcgacggcg tggcgcccga agccgcgttt cacttcacgc aggctcccgt
     7201 cgaggacttt gcgcgagcgg tcgatgaggc agatgtcgtc gcacacctcc tcggcgaccg
     7261 acatctggtg cgtcgagaag atgacggtct tgcccgcgct tttgagttca agcacgatgc
     7321 ctttcaacaa ctccgcgctc acggggtcga ggccggagaa gggttcgtcg aggatgacga
     7381 ggtccgggtc gtgcagcacg gcggcgacga actgcacctt ctgctgcatg cccttcgaca
     7441 gctccgaagc cttcttctgc ttccagtcgg cgagcttcac cttgtcgagc cagcggtcga
     7501 tggcttcgtc ggcgcggcgg cggtcgaggc tcttcagctc gccgaagaag cggagctgtt
     7561 cgcccacctt catcttggcg tacagcccgc gctcttcggg gagatacccg atgcgctcct
     7621 gcgtctgcgc ggagacaggc tcgccgaaca gctcgatggt gccctcgtcg ggcgcggtga
     7681 tgccgacgat catgcgaatc gtggtggtct tgcccgcccc gttcggcccg atgagtccga
     7741 agattctgcc ggcgcggacg cgcagggaga ggtcgccgac ggcggtgaag tcgtcgaacc
     7801 tcttggtgac gcgttcgagt cggagcgtga cggcgttttc gtcagcggcg ggcttcatcg
     7861 gcctccggcg gtgaagaagt tagagcgcga gttattacga gaagcggcgg gcgaaagttt
     7921 tttgccggaa gatttgaaca taacgggtga ctcctctgcg ccctctgcgc tcctccgcgt
     7981 cctctgcgtt gaatcaccct tcgcgcgtta ctcaacgaac cggagtcgga gatgacgaaa
     8041 ccccaaccct ccgagcagag ggacgcggca caggccgacc gcctcgtctc gctggacgcg
     8101 ttccgcgggc tgaccatcgc gggcatgatc ctcgtcaaca accccggcac gtggggcgcc
     8161 atctacgacc ccttggagca cgccgcgtgg cac
//
LOCUS       DBNGOH010000711         4434 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_289488, whole genome shotgun sequence.
ACCESSION   DBNGOH010000711 DBNGOH010000000
VERSION     DBNGOH010000711.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4434)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4434)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4434
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_289488"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            166..1956
                     /locus_tag="AC159P_13795"
     CDS             166..1956
                     /locus_tag="AC159P_13795"
                     /inference="COORDINATES: protein motif:HMM:NF024821.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3696568.1"
                     /translation="MRPRNRPRAARLTLLAALLACALPHAARAQPPQQQPQQPPTHAF
                     MAHYERGVEHQQAGRFREAAEEFKSAVRLKPDFLPAHVGLGLAYGRAGEHAQAVESFR
                     HVLRLQPGVAELHYNLGTAYANWGKDEEAVKAYEEALRLKPDWAGAMAALGSTRSRLG
                     RHEEAITLLHRATVLQPDLPELHHLLATAYSAAGQRDAAVEHFRRVVALSPDSPPAHF
                     NLGHALKDAGKHQEAVAAFTSGLTLKEDAEAFFHLGLLHAEAGRQAEAATAYEHAVRL
                     APSMQGAHFNLGLAYVSLAKHEKAVEAFAASVRLKPDSTESHINLAAAHAQLGRHAAA
                     AAAMEGFVRLKPDDPEGHFQVGNEYMMAGQFDKALAALREALRLKPDHAMALDRLRIT
                     HARIEGAAEALALKQRIERDPKDEYALARLAFLYYFTGRYDESIELFKKRLRLTPNDP
                     EVIHRLAITYSEAGRNEEAIEHYKRAISLKPPHHVLPWSLARSYIELGRHEEAVAALN
                     DSLKLNPDFAFALYDLGMTHKALGKYEEAAEAFRRLLAVEPRHVNGNFELGLAYLQLG
                     DRLRAEQQYQLLRDLDPNSARDLYSRLHRK"
     gene            complement(1981..2784)
                     /locus_tag="AC159P_13800"
     CDS             complement(1981..2784)
                     /locus_tag="AC159P_13800"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3696569.1"
                     /translation="MKKSPESGVWSLKSEQADSERSASVQSSDSGLRTPDSGLVVEDL
                     RKGFRGAAGGVVEVLRGVSFAAGAGEMLAVVGASGAGKSTLLHVLGGLEAADAGSARL
                     GGFDITRAGAGALARFRRSEVGFVFQSHRLLADLSAEENVALPLLVARWKRGAALGAA
                     REMLAAVGLAGRERHAAGELSGGEQQRAGLARALVTRPRLVLADEPTGNLDARTGAEV
                     SALLFALCREARACAVVATHNERLARSCDRVLTLREGRVSEESGTKDER"
     gene            complement(2781..4073)
                     /locus_tag="AC159P_13805"
     CDS             complement(2781..4073)
                     /locus_tag="AC159P_13805"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HIR3696570.1"
                     /translation="MPFELFLALRYLRMRRGRRVVKWTAGAALVGIACGVAALVVALA
                     LANGFRDELREKILSGTAHVTVTREGRATIDETRAVVARVRAVEGVTDASPTTYEGVL
                     LSGPTGAAYAVLRGIDAESRRAREAVRRTLVAGAVEPLFEDARAGGSGAAEEARGEAD
                     EAREAPVRIIVGEELAARTGLGRVGEEGWALTGEKARNELGFEPRATRVRVAGVFRAG
                     LYDYDAAWAYVSLDEAARVAGEGLKSAVVSVEVADVYAAGKAAARVRRELGGGGWTTV
                     DWREANRPLFAALELERRTVALIIMLIMLLAALNITTALALVVVERRADIAVLNALGA
                     GARSVTTIFMIEGAALGLAGALAGVALGLAACAAANHFGLIRLPPDVYSISAVTLRPH
                     AADVVWPALAAFAVSLLATVYPALQAARVRPAEALRYE"
     gene            complement(4192..>4434)
                     /locus_tag="AC159P_13810"
     CDS             complement(4192..>4434)
                     /locus_tag="AC159P_13810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696571.1"
                     /translation="VPVIQEHLKQGRRVFLDADPRLWATDGWQVSETRAVATLERDFR
                     FRRAADRLYEIRPPEDASAQDRPNLAGLLPENRKKP"
BASE COUNT          729 a         1787 c         1345 g          573 t
ORIGIN      
        1 acaccatgcg aaactcagag agaagaaaaa tcggcgccgc ccgcgccgtt cccgaaaccc
       61 cgcgccctga ggtaagctaa cccgcgccgt ggccgcccct cgcgcgcggc tctccggcaa
      121 acgccccacg gcaaactccc gttcgagcac gaggacacac ttcacatgcg accacggaac
      181 aggccccgcg ccgcacgcct taccctcctc gccgcgctcc tcgcgtgcgc gctcccgcac
      241 gcggcgcgcg cgcagccgcc gcagcaacag ccgcaacagc cgcccacgca cgccttcatg
      301 gctcactacg agaggggcgt cgagcaccag caggccggtc gcttccgcga ggccgccgaa
      361 gagttcaaga gcgccgtccg gctcaagccc gacttcctcc ccgcgcacgt cggcctcggc
      421 ctcgcttacg ggcgggcggg cgaacacgcg caggccgtcg aatcgttccg ccacgtgctc
      481 aggctccaac ccggcgtggc cgagctgcac tacaacctcg gcacggccta cgccaactgg
      541 gggaaggacg aggaggcggt caaggcgtac gaagaggctt tgcgactcaa gcccgactgg
      601 gcgggggcga tggccgcgct cggctccacg cgcagccgcc tcggtcggca cgaagaggcc
      661 atcaccctcc tccaccgcgc gacggtgctc cagcccgact tgccggagct gcaccatctg
      721 ctcgccaccg cctacagcgc cgcgggccag cgcgacgccg ccgtcgaaca cttccgccgc
      781 gtcgtcgcgc tcagccccga ctcgccccct gcgcacttca acctcggcca cgcgctcaag
      841 gacgcgggca agcatcaaga ggccgtcgcc gctttcacca gtggactcac gctcaaagag
      901 gacgccgaag cctttttcca cctcggcctg ctccacgccg aagccggtcg gcaagccgaa
      961 gcggccacgg cctacgagca cgccgtccgc ctcgcgccct cgatgcaggg cgcgcacttc
     1021 aacctcggtc tcgcttacgt cagtctggcg aagcacgaga aagcggtgga ggcgttcgcc
     1081 gcgtccgtcc gcctcaagcc cgacagcacc gagtcgcaca tcaacctcgc cgccgcacac
     1141 gctcagttgg ggcggcacgc cgccgccgct gccgcgatgg aaggcttcgt gcggctcaag
     1201 cccgacgacc ctgaaggcca ctttcaggtc ggcaacgagt acatgatggc gggccagttc
     1261 gacaaggcgc tggccgcgct ccgcgaggct ttacgcctca agcccgacca cgcgatggcg
     1321 ctcgaccgcc tgcgcatcac ccacgccaga atcgagggcg ccgccgaagc cctcgcgctc
     1381 aaacagagga tcgagcgcga ccccaaagac gagtacgcgc tcgcccgtct cgccttcctc
     1441 tactacttca cgggccgcta cgacgaatcg atcgagctgt tcaagaagcg gctccggctc
     1501 acgcccaacg accccgaagt gatccaccgc ctcgccatca cctactccga ggcgggccgc
     1561 aacgaagagg ccatcgaaca ctacaagcgc gccatcagcc tcaagccgcc gcaccacgtc
     1621 ctcccgtgga gcctcgcccg ctcctacatc gaactcggtc ggcacgaaga ggccgtcgcc
     1681 gcgctcaacg attcgctcaa gctcaacccc gatttcgcct tcgcgctcta cgacctcggg
     1741 atgacgcaca aggcgctcgg caaatacgaa gaggcggcgg aagccttccg ccgcctgctc
     1801 gccgtcgagc cgcgccacgt caacggcaac ttcgaactcg gactcgccta cctccaactc
     1861 ggcgaccgtc tccgcgccga gcagcagtac caactcctcc gcgacctcga ccccaactcc
     1921 gcccgcgacc tctactcacg tctccaccgg aagtgacgcg cgcggacacg cccgttcact
     1981 ttacctctca tctttcgtcc cgctctcttc gctcacgcgc ccttcgcgca gcgtgagcac
     2041 gcggtcgcac gagcgcgcca gccgctcgtt gtgcgtggcg acgaccgcgc aggcgcgcgc
     2101 ctcgcggcag agcgcgaaca gcagcgccga gacctccgcg cccgtgcgcg cgtcgaggtt
     2161 gccggtcggc tcgtccgcga gcacgaggcg cgggcgcgtc acgagcgcgc gcgccagccc
     2221 cgcgcgctgc tgctctccgc ccgacagctc gcccgccgcg tgcctctctc ttccggcgag
     2281 tccgaccgcc gccagcatct cgcgcgcagc gcccaatgcc gcgccgcgct tccacctcgc
     2341 caccagcagc ggcagcgcga cgttctcctc cgccgagagg tctgcgagca gccggtgcga
     2401 ctggaagacg aagccgacct cgcttcggcg gaagcgcgcg agcgcgcccg cgcccgcccg
     2461 cgtgatgtcg aacccgccga gccgcgccga gcccgcgtcc gccgcctcaa gccccccgag
     2521 cacgtgcagc agcgtcgatt tgcccgcgcc cgaagcgccc accaccgcca gcatctcgcc
     2581 cgcccccgcc gcgaacgaca cgccccgcaa aacttcgacc acgcccccgg cggccccgcg
     2641 gaaacccttc cgcaaatctt cgaccacgag tccggagtcc ggagtccgga gtccggagtc
     2701 agaagattgc accgacgccg agcgctccga gtccgcctgc tcggacttca gactccaaac
     2761 tccggactcc ggactcttct tcattcgtac cgcaaagcct ccgcgggccg cacgcgcgcc
     2821 gcctgaagcg cggggtagac ggtcgccagc aggctcacgg cgaaggccgc gagcgcgggc
     2881 cacaccacgt ccgccgcgtg cgggcggagc gtcaccgcgc tgatcgagta aacgtcgggc
     2941 gggagccgga tcagcccgaa gtggttcgcg gcggcgcagg ccgcgaggcc gagcgcgacc
     3001 cccgcgagcg cgcccgcgag gcccagagcc gcgccctcga tcatgaagat cgtggtgacg
     3061 ctgcgcgcgc ccgcgccgag ggcattgagg acggcgatgt ccgcgcgccg ctcgacgacg
     3121 acgagcgcga gcgcggtcgt gatgttgagc gccgcgagca gcatgatgag catgatgatg
     3181 agggcgaccg tgcgccgctc aagctcaagc gccgcgaaca gcgggcggtt cgcctcgcgc
     3241 caatcgaccg tcgtccagcc cccgccccca agctcgcgcc gcacacgcgc cgccgccttg
     3301 cccgccgcgt acacgtccgc gacctcgacg ctcaccaccg ccgacttcaa cccctcgccc
     3361 gcgacgcgcg ccgcctcgtc cagagagacg taagcccacg ccgcgtcgta gtcgtacaac
     3421 cccgcgcgga acacgcctgc cacgcgcacg cgcgtcgccc gcggctcgaa gcccagctca
     3481 ttgcgcgcct tctcgcccgt caaggcccaa ccctcttcgc ccacgcgccc caaccccgtc
     3541 cgcgccgcca gctcttcgcc gacgataatt cgaacgggcg cctccctcgc ttcgtccgcc
     3601 tcgccccgcg cctcttccgc cgcgcccgac ccgcccgctc tcgcgtcttc gaacagcggc
     3661 tcgaccgcgc ccgcgacaag cgtgcgccgc acggcctctc gggcgcgccg cgactcggca
     3721 tcgatgccgc gcagcaccgc gtaggccgcg cccgtggggc cgctcagcag gacgccctcg
     3781 taggtggtcg gcgacgcgtc cgtcacgccc tcgaccgcgc gcacgcgcgc gacgacggcg
     3841 cgcgtctcgt cgatggtcgc ccggccctcg cgcgtcacgg tgacgtgcgc cgtcccgctc
     3901 agaatcttct cgcgcagctc gtcccgaaag ccgttcgcca gcgccagcgc cacgaccagc
     3961 gccgccaccc cgcacgcgat cccgacgagc gccgcacccg ccgtccactt caccacgcga
     4021 cgcccccgcc gcatccgcaa atacctgagc gcgagaaata gttcgaaagg catatcgaaa
     4081 gccgtgaacc gaaaatcgta agccgtgacg agtgaagaac cgcgagccgt cagccgcaaa
     4141 tcgtcgcatg agaagaccgg ttcttactta tcacggttca cggttttcgg ttcacggctt
     4201 tttcctgttc tccggcagga ggccggcgag gttcgggcgg tcttgcgccg aggcgtcttc
     4261 gggggggcgg atttcgtaga ggcggtcggc ggcgcggcgg aagcggaagt cgcgttcgag
     4321 ggtggcgacg gcgcgcgtct cgctgacctg ccagccgtcg gtggcccaga ggcgcgggtc
     4381 tgcgtcgagg aagacgcggc ggccttgctt caagtgctct tgaatgacag gcac
//
LOCUS       DBNGOH010000712         3653 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1389756, whole genome shotgun sequence.
ACCESSION   DBNGOH010000712 DBNGOH010000000
VERSION     DBNGOH010000712.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3653)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3653)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3653
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1389756"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            395..1156
                     /locus_tag="AC159P_13815"
     CDS             395..1156
                     /locus_tag="AC159P_13815"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3696572.1"
                     /translation="MEWHIPEAAGFAESKGLRVLEIGCGLGTDGAGFARAGAVYTGVD
                     LTEAAVALARRRFELEGLPGEFRVADGEALEFADGSFDVVYSHGVLHHTPDTAGAVRE
                     VHRVLKPGGRAVVMLYHRDSYNYRVNIGVLRRVGAHLLKTGAGLRLAHRLTGEPVESL
                     REHAARLKEDAGQYLSPDEFLSRNTDGAGNPLTRVYSRREARALFRDFAEVKFALHFL
                     NKRWLPLLGPLLPRSVEARLASRWGWHLWIYATKQ"
     gene            1271..2251
                     /locus_tag="AC159P_13820"
     CDS             1271..2251
                     /locus_tag="AC159P_13820"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013907827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HIR3696573.1"
                     /translation="MRNILVTGGAGFIGSHLVERLLGEGRWRVTVVDDFNDFYDPSIK
                     RANVRPHEGREDFRLVEADIRDRAALGRVFDGEQFDVIVHLAARAGVRPSLAEPVLYT
                     ETNIGGTVNLLELARAHGVRQFVFGSSSSVYGENEKVPFAEDDPNFNPISPYAATKAA
                     GELLCHTYSHLHGLRCVCLRFFTVYGARQRPDLAIHKFARLISAGRPVPVYGDGTTRR
                     DYTYVDDIIAGVRAAVDYEASGYEVINLGESRTVELRELIALLEKELGREAVIDRQPA
                     QPGDVPRTFADITKARRLLGYSPQTQIEDGIRKFVEWYKGNAERRVQNDE"
     gene            2318..3373
                     /locus_tag="AC159P_13825"
     CDS             2318..3373
                     /locus_tag="AC159P_13825"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR3696574.1"
                     /translation="MKIYDEEETAISRPAAVVEAEEVTVAEERVAADAAPEISVFLPV
                     LNEEPNLRPLHAKLDESLATLGHTAEIIYVDDGSTDGSLEVLKELAARDPRVRVIALR
                     RNYGQTAAMAAGIDAARGEVLIPMDADLQNDPVDIARLMAKLDEGFDVVSGWRKERQD
                     KMVTRKVPSAIANWIISFIGGVKLHDYGCSLKAYRREFLKEVKLYGEMHRFIPIYASW
                     VGARVTEIPVRHHARTMGKSKYGLSRTFKVMLDLMTIKFMASYHTKPIYVFGMFGFSS
                     FVISLIAFVWACLRKFYWDESFIRNPLLTISIVMFAVGSQFILMGLLAEMLVRTYHES
                     QAKTIYVVRERVGFRGE"
     gene            3467..>3653
                     /locus_tag="AC159P_13830"
     CDS             3467..>3653
                     /locus_tag="AC159P_13830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014018842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696575.1"
                     /translation="MDGGLERVYETAWAWSRARGHAGHDPFDALNSRLFQATPLRRSR
                     LARLAWTQAFNRSPVNLR"
BASE COUNT          587 a         1100 c         1298 g          668 t
ORIGIN      
        1 gatacctgcc ggcagtgtca tcgcgccgat cagacaactc cgcgcggcat gatcccggcg
       61 cggtcgtgat tataccgccc cgcgcgcctg tgccggacaa gacttgagcc gaaagggccg
      121 gcgcttcggg ctgcttgttg agcatgctcg cggcgtgtta ctttttcacg ttttgggagg
      181 ggtggaattt ttgctctttg cgctccgttg agaagggagc gcagcaggct ctgtccaagt
      241 gtccgaaagc tcgttggtga tggcgaacgc ggaaaacatt ttgaaggagc gtgtgcgggc
      301 gttttggcag gcgcatccgt gtggcacgaa gtttactgat gcgcaggagg ggtcgcgggc
      361 gttcttcgag cgggtggagg agcaccggta tagggtggag tggcatattc cggaggcggc
      421 agggttcgcg gagtcgaagg ggttgagggt gttggagatc gggtgcgggt tggggacgga
      481 cggggcgggg ttcgcgcggg cgggggcggt gtatacgggg gtggatttga cggaggcggc
      541 ggtggcgttg gcgcggcggc ggttcgagct ggaagggctg ccgggcgagt ttcgtgtggc
      601 ggatggggag gctttggagt tcgcggacgg gtcgttcgat gtcgtctatt cgcacggggt
      661 gttgcatcac acgccggaca cggcgggggc tgtgcgcgag gtgcatcggg tgttgaagcc
      721 gggggggcgg gcggtggtga tgttgtatca ccgcgactcg tacaactacc gcgtgaacat
      781 aggggtcttg cggcgggtgg gggcgcattt gttgaagacg ggggcgggcc tcaggctggc
      841 gcaccgtctg acgggcgagc cggtggagag tctgcgggag cacgcggcgc ggctcaagga
      901 ggacgcgggg cagtatcttt cgccggacga gttcctgagc cggaacacgg acggcgcggg
      961 caaccctctg acacgcgtct actcgcggcg cgaggcgcgc gcgctgttca gggatttcgc
     1021 ggaggtgaag ttcgcgttgc acttcctgaa caagcgctgg ctcccactgc tggggccgct
     1081 gctgccgcgc tccgtcgagg cgcggctcgc gtcgcggtgg ggttggcact tgtggattta
     1141 cgcgacaaag cagtaggcag taggcagtcg attgactgag gctcgcgttg acgctggcac
     1201 cctgactgct cgatagagat ggtttttaac tgcctaccgc ctaccgccta ctgcctactt
     1261 ggttacttga atgagaaaca tactggtcac gggcggggcg ggcttcatcg ggtcgcatct
     1321 ggtggagcgt ctgctgggcg aggggcgctg gcgtgtgacg gtcgttgacg acttcaacga
     1381 cttctacgac ccttcgatca agcgcgcgaa cgtgcgcccg cacgaggggc gcgaagactt
     1441 ccgtttggtc gaggccgaca tccgcgaccg cgcggcgctc gggcgcgtgt tcgacggcga
     1501 gcagttcgat gtgatcgtgc atctggcggc gcgggcgggc gtgcggccct cgctggccga
     1561 gccggtgctg tacacggaga cgaacatcgg cgggacggtg aacctgttgg agctggcgcg
     1621 cgcgcacggc gtcaggcagt tcgtcttcgg ctcttcgtct tccgtttacg gcgagaacga
     1681 gaaggtgccg ttcgccgagg acgacccgaa cttcaacccc atctcgccgt acgcggcgac
     1741 gaaggcggcg ggcgagctgc tgtgccacac ctattcgcac ctgcacgggc tgcgctgcgt
     1801 ctgcctgcgg ttcttcaccg tctacggcgc gcggcagcgg cccgacctcg ccatccacaa
     1861 gttcgcgcgg cttatctcgg cgggccggcc cgtccccgtc tacggcgacg gcacgacgcg
     1921 gcgcgactac acctacgtcg atgacatcat cgcgggcgtg cgcgcggcgg tcgattacga
     1981 ggcgagcggc tacgaggtca tcaacctcgg cgagtcgcgg acggtcgagc tgcgggagct
     2041 gatcgcgctg ctcgaaaaag aactcgggcg cgaggccgtc atcgaccgcc agcccgcgca
     2101 gcccggcgac gtgccgcgca ccttcgccga catcacgaag gcgcggcgcc tgctcggcta
     2161 cagcccgcag acgcagatcg aggacggcat acgcaagttc gtcgagtggt ataaaggaaa
     2221 tgcagaacgc agagtgcaga atgatgaatg agaggcagct tgttttttat tcatcattct
     2281 gcactctgcg ttcatcatta tttccgaggg ttttgtgatg aagatttacg acgaggaaga
     2341 gaccgccatc agccgaccgg cggcggtcgt cgaagctgag gaagtgacgg tcgccgaaga
     2401 gcgcgttgcc gcggacgcgg cgccggagat ttccgtcttc ctgcccgtgc tcaacgagga
     2461 gccgaacctg cgcccgctgc acgcgaagct cgacgagtcg ctggccacgc tcggccacac
     2521 cgccgagatc atctacgtgg acgacggctc gaccgacggc agcctcgaag tcctcaagga
     2581 actggcggcg cgcgacccgc gcgtgcgcgt catcgcgctc aggcgcaact acgggcagac
     2641 cgccgcgatg gccgcgggca tcgacgccgc gcgcggcgag gtgctgatcc cgatggacgc
     2701 cgacttgcag aacgacccgg tggacatcgc gcggctgatg gccaagctcg acgagggctt
     2761 cgacgtggtc tcgggctggc gcaaggagcg gcaggacaag atggtcacgc gcaaagtccc
     2821 ttcggccatc gccaactgga tcatctcgtt catcggcggc gtcaagctcc acgactacgg
     2881 gtgctctctg aaagcctacc ggcgcgagtt cctgaaggag gtcaagctct acggcgagat
     2941 gcaccgcttc atcccgatct acgccagttg ggtcggcgcg cgcgtcacgg agattcccgt
     3001 ccggcaccac gcgcgcacga tgggcaagtc gaagtacgga ttgtcgcgca ccttcaaggt
     3061 gatgctcgat ctgatgacga tcaagttcat ggcctcgtac cacacgaagc cgatctacgt
     3121 cttcgggatg ttcggcttct cctcgttcgt catctcgctg atcgccttcg tctgggcgtg
     3181 cctgcgtaag ttctattggg acgagtcctt catccgcaac ccgctcctca ccatctccat
     3241 cgtcatgttc gccgtcggct cgcagttcat cctcatgggc ctgctggccg agatgctcgt
     3301 gcgcacctac cacgagtcgc aggccaagac catctacgtc gtgcgcgagc gcgtcggctt
     3361 caggggcgag tgacgcgccg acggttcggc agggcggcgg cggcgacgac ccgccgcggc
     3421 gcacgcgtcc tccgcgcgcc tcgccgtctg ttatcatcgg gcgggaatgg acggcgggct
     3481 tgagcgcgtg tacgagacgg cctgggcgtg gagccgcgcg cgcggccacg ccgggcacga
     3541 ccctttcgac gcgctcaaca gccgcctctt ccaagccaca cctttgcgcc gctcgcgcct
     3601 cgcgcggctc gcctggacgc aagccttcaa tcgctcgccc gtcaacctgc gcc
//
LOCUS       DBNGOH010000713         3098 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1207493, whole genome shotgun sequence.
ACCESSION   DBNGOH010000713 DBNGOH010000000
VERSION     DBNGOH010000713.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3098)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3098)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3098
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1207493"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..304
                     /locus_tag="AC159P_13835"
     CDS             <1..304
                     /locus_tag="AC159P_13835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HIR3696576.1"
                     /translation="GLRPGVALDEQSLDDLTALRTLGADRESRTGTPWYELYDGERVA
                     LFGHWPAAVPRRGPRAIGLDTGCVYGYALTAYVVESDKFISVPARRAYDPPRDRVG"
     gene            483..1166
                     /locus_tag="AC159P_13840"
     CDS             483..1166
                     /locus_tag="AC159P_13840"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020147811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - dTMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HIR3696577.1"
                     /translation="MTMTAYGQHRFPGKLFVVEGTDGSGKSTQLALLYQWLKAEGHHV
                     FFSEWNSSPLVKDTTRRAKKKHLFTPTTFSLLHATDFADRTERDIIPPLKAGAIVLAD
                     RYIYTAFARDVARGCDRAWVREVYRFAVRPTVAFFFRAPLDVAIDRIVSGRPQLKHYE
                     AGLDMQWTDDPEESFTIFQGKILEEYDRMVAEFGLTVIDATRKIERQQREMRKIVMRH
                     LEGEAEEAA"
     gene            1166..1912
                     /locus_tag="AC159P_13845"
     CDS             1166..1912
                     /locus_tag="AC159P_13845"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - dTMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HIR3696578.1"
                     /translation="MREVKFYGTPLPGVEHEAYAGKLIVVEGTDGVGRSTHITLLRSW
                     LESRGHAVLDTGMTRSVLAARGIKEAKTGTTLGRLTMQLFYATDFADRLENEMLPALR
                     AGFVVLTDRYIYSAIARASVRGVDPRWVRSIYGIALVPDLILYLRIHRADELVSRVLA
                     SGRKFDYWESGMDLHLGEDFYDSFVAYQARLLKEFDAMSGEYGFRVVDASRSIRRVAA
                     DLRRAVARVIERDEAAQVSDEEEAVSASTG"
     gene            2084..>3098
                     /locus_tag="AC159P_13850"
     CDS             2084..>3098
                     /locus_tag="AC159P_13850"
                     /inference="COORDINATES: protein motif:HMM:NF014587.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HIR3696579.1"
                     /translation="MKLAAIDIGSNSIKLVVVEAAGPDSFAVLAREKEVVRLGQDTLR
                     QGYLAPAAIERACDTMKRFRSIAEARGAERVLATATASVREASNAAEFIEEVERRAGV
                     RVEVLSGIEEARLIGIAAAHGCARTQGAALVNVDIGGGSTEISLMKDGVPVELYSLKL
                     GAVGLTERHVASDPPARKELRALRGQIRAALERPARELAGARWQYATGTSGTIIAVGE
                     VLHRRALREAGRAEQGAQAPGAEIALGQLEKLNERFASMSAEERRAVPGVSHQRAEII
                     VACGQILEGVMQALSINHLRTCDYALREGVVIDTLRQLEAERLPPVPDTSDPRLRGAH
                     AVGR"
BASE COUNT          500 a         1058 c         1060 g          480 t
ORIGIN      
        1 gggcttgcga cccggggtgg ccctggacga acagtcgctc gacgacctca cggcgctgcg
       61 cacgctcggc gccgaccgcg agagccgcac gggcacgccc tggtacgaac tctacgacgg
      121 cgagcgggtc gcgctcttcg gacactggcc ggccgccgtt ccgcgccgcg gcccacgcgc
      181 catcggcctc gacaccggct gcgtctacgg ctacgcgctc acggcctacg tcgtcgagtc
      241 ggacaagttc atcagcgtcc ccgcgcgccg cgcctacgac ccgccgcgcg accgcgtcgg
      301 ctgattgcgg aatgcggaat gcggaatgcg gattgaaaag caggcatggt gtgagggatg
      361 atgagaaggc tgctgttttt cgtcacgcat tctcttgacg agaggcgcgc ttctttcaat
      421 ccgcattccg cattccgcaa tcgttggact gttacgccgt tgtaacagcc gggcattaag
      481 ctatgacgat gaccgcatac ggacagcatc gcttccccgg caagcttttc gtcgtcgagg
      541 gcacggacgg gagcggcaaa tcgacgcagc tcgcgctgct ctaccagtgg ctcaaggccg
      601 agggccacca cgtcttcttc tccgagtgga actcttcacc gctcgtcaag gacacgaccc
      661 gccgcgccaa gaagaagcac ctcttcacgc cgaccacctt ctcgctcctg cacgccaccg
      721 acttcgccga ccgcaccgag cgcgacatca tcccgcccct gaaggcgggc gcgattgtcc
      781 tcgccgaccg ctacatctac acggcgttcg cgcgcgacgt ggcgcgcggc tgcgaccgcg
      841 cgtgggtgcg cgaggtctac cgcttcgccg tccgcccgac ggtcgccttc ttcttccgcg
      901 cgcctttgga cgtggcgatc gaccgcatcg tgtcgggccg cccgcagctc aagcactacg
      961 aggccggcct cgacatgcag tggacggacg accccgagga gagcttcacc atcttccagg
     1021 ggaagattct cgaagagtac gaccggatgg tcgcggagtt cggcctcacg gtcatcgacg
     1081 cgacgcgcaa gatcgagcgg cagcagcgcg agatgcgcaa gatcgtcatg cggcacctcg
     1141 aaggcgaggc ggaggaggcg gcctgatgcg cgaggtgaag ttctacggca cgccgctgcc
     1201 gggcgtcgag cacgaggcgt acgcgggcaa gctcatcgtc gtcgagggca cggacggggt
     1261 ggggcgctcg acgcacatca cgctgctccg ctcgtggctg gagtcgcgcg gccacgccgt
     1321 tctcgacacc ggcatgacgc gctccgtgct cgccgcgcgc ggcatcaagg aggcgaagac
     1381 cggcaccacg ctcgggcgcc tgacgatgca gctcttctac gcgaccgact tcgccgaccg
     1441 gctggagaac gagatgctcc cggccttgcg cgcgggcttc gtcgtcctca ccgaccgcta
     1501 catctactcg gcgatcgcgc gcgcctcggt gcgcggcgtg gacccgcgct gggtgcgctc
     1561 gatctacggc atcgcgctcg tgcccgatct gattctgtat ctgcgcatcc accgcgccga
     1621 cgagctggtc tcgcgcgtgc tcgcctccgg cagaaagttc gactactggg agtcgggcat
     1681 ggacctgcac ctcggcgagg atttctacga cagcttcgtc gcctaccagg cgcggctcct
     1741 caaggagttc gacgcgatga gcggcgagta cggcttccgc gtcgtggacg cctcgcgctc
     1801 gatccgccgc gtcgccgccg acctgcgccg cgccgtcgcg cgcgtcatcg agcgcgacga
     1861 ggcggcgcaa gtgtctgacg aggaagaagc ggtctcggca tctacaggtt gagtgtcagg
     1921 caggatgtat ttcaacacag agggcacgga ggagacacgg agggcacaga gaaacctatt
     1981 tcttctccgt gaactctgtg catcctccgt gccctctgtg ttgaaatgaa cgttccacac
     2041 actcggctga aagttatact gtttcgcacg gagccttaga gcgttgaaac tcgcggccat
     2101 cgacatcggc tcgaactcga tcaagctcgt cgtcgtcgag gcggcagggc cggactcgtt
     2161 cgccgtgctg gcgcgcgaga aggaggtcgt gcgcctcggg caggacactc tgcggcaggg
     2221 gtatctggcg cccgccgcca tcgaacgcgc gtgcgacacg atgaagcgct tccgctccat
     2281 cgccgaggcg cgcggggccg agcgcgtgct cgccacggcg accgcttctg tgcgcgaggc
     2341 gtccaacgcc gccgagttca tcgaagaggt ggagcggcgt gcgggcgtgc gcgtcgaggt
     2401 tctgtcgggc atcgaggagg cgcggctcat cggcatcgcc gcggcgcacg gctgcgcgcg
     2461 aacgcagggc gccgcgctcg tcaacgtgga catcggcggc ggctcgaccg aaatctcgct
     2521 gatgaaggac ggcgtgccgg tcgaactcta ttcgctcaag ctcggcgcgg tcggactcac
     2581 cgagcggcac gtcgcgagcg acccgcccgc gcggaaggag ctgcgcgcgc tgcgcggcca
     2641 gatacgcgcc gcgctcgaac gaccggcgcg cgaactggcg ggcgcgcgct ggcagtacgc
     2701 gaccggaacc tccggcacca tcatcgcggt cggcgaggtc ttgcaccgcc gcgcgctgcg
     2761 cgaggccgga agggccgagc agggcgcgca ggcgccgggc gcggagatcg cgctggggca
     2821 actggaaaag ctgaacgagc gtttcgcgtc gatgagcgcg gaggagcggc gggcggtgcc
     2881 gggcgtcagc caccagcgcg cggagatcat cgtcgcgtgc gggcagattc tggaaggcgt
     2941 gatgcaggcg ctctcgatca accacctgcg gacgtgcgac tacgcgctgc gcgagggcgt
     3001 cgtcatcgac acgctgcggc agctcgaggc cgagcggctg ccgcccgtgc ccgacacctc
     3061 cgacccgcgc ctgcgcggcg cgcacgccgt cggtcgcc
//
LOCUS       DBNGOH010000714         4092 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1024248, whole genome shotgun sequence.
ACCESSION   DBNGOH010000714 DBNGOH010000000
VERSION     DBNGOH010000714.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4092)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4092)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4092
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1024248"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            169..1773
                     /gene="ccsA"
                     /locus_tag="AC159P_13855"
     CDS             169..1773
                     /gene="ccsA"
                     /locus_tag="AC159P_13855"
                     /inference="COORDINATES: protein motif:HMM:NF013725.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HIR3696580.1"
                     /translation="MSRVLKQTPVALAPRIEFEASAVASYLPAVLAVGGSALLTLWRL
                     RAGGEHFISDGALMLLALACYLTAAIFHLTDLYAPSSLFRRIGLWTATAGVFFNLASW
                     GVRWVTGYERELAVIARQGGEMPWAFRYIPFTNLYDMSLAFAFGAGLATLLIAHRQSF
                     RFLGALSLPLAALILVLARFIGGEIANLPAVLDSYWRPIHVGTASLSYGVALVCFAVA
                     VVYLLKDGVKTEAMALWTSGFALAVFAAVSRFSVFAPATFGTYSASTMFGSSKMSLPL
                     RADIPYVGWMIVLSAVLLAGVVVAFARYLAKKDQRARRWGHSLLKLSLVAQAATVAVL
                     VSQLKSITGVVAALDPRQYARFGAYLLEQDGMSAAQVSMLAPSRLESVAADFVRSRGD
                     ELFLSLRANPVELSALITAAAATLFVIIFSFRTERLRAGLPSLEKIDSLMYKTAGITF
                     AGLALLLITGAVWANESWGRYWGWDPKEVGALVAWLTYGAFLHVRISRGWTGRPAAYF
                     ALVAFLLILFTYLGVSYLLPGMHSYA"
     gene            1799..3136
                     /gene="hemA"
                     /locus_tag="AC159P_13860"
     CDS             1799..3136
                     /gene="hemA"
                     /locus_tag="AC159P_13860"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HIR3696581.1"
                     /translation="MSLVLTGVSHKTAPVEVRERLAFARNALPESLRSLVDGRVVREG
                     MILSTCNRVELLVDADPARLTEARARVSEFFSTRAEDLDEELGGHVYTKEDDEAVRHL
                     LRVASSLDSMMLGEPQILGQVRQAYTDACDAGTAGRTLHRLLHRAFHAAKRVRTETNI
                     ASSAVSVSFAAVELGRKILGTLEGRTVFLVGAGEMAELAARHLAKNGASRILFANRTH
                     ETARRLAAEFGGESVPYERFPAVLAEADIVICSTGASCHIITPRMARAALKLRRNRPS
                     FFIDISVPRNVDPKVGEVTNLFVFDVDDLEAVVASNLREREREAARAEEIVETEAAEF
                     LKALHRLDIGPTVLGLKERLRAIAREEFRRRRSSLGDLSPEQERAVEQVLAATVNKIS
                     HPLIQRLRRSFDTGDDGAVRAWRDSFNLGDESEIRASVQTALARTFNLRTVIA"
     gene            3219..3971
                     /locus_tag="AC159P_13865"
     CDS             3219..3971
                     /locus_tag="AC159P_13865"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3696582.1"
                     /translation="MKKDNILYGIIGVLVGFIVGFAFANTTNQRGYAAQSTTAGQPQQ
                     TAGLPPDHPPIDGASAGPNAAAVGAAMKLASEQPGNFDAQVKAAEVAYAAHRYDDAIK
                     IFTLANQLRPEQFDVLVGLGNTNFDAERFEEAEKWYIAALRQQPDNVNVRTDLGLTFF
                     FREPRDVERAVKEYRASLERDPAHIPTLQNLTVALTAKGDADAARAALSKLESLSPQN
                     QALPRLRADLEKLDAPGRTATNKTSAADMGGR"
BASE COUNT          684 a         1429 c         1292 g          687 t
ORIGIN      
        1 cgccgtcttc ctcttctcgc accagcgggt gtgggcgctc gtcgagccgc gcggcgaagg
       61 gcgctgtgaa atcactctgg ggggcaacac caaccgcaac cgtctcggct ttgaagaccg
      121 cttcaagcgg ctcgtcaagg agttgagcac cacggaggtc agttgattat gagtagagtc
      181 ctgaaacaaa cgcccgtcgc tctcgccccg cggatcgagt tcgaagcctc ggcggtcgcc
      241 tcatatctgc ccgccgttct cgccgtcggc ggctcggccc tgctcaccct ctggcgcctg
      301 cgcgcggggg gcgagcattt catctcggac ggcgcactca tgcttctggc gctcgcctgc
      361 tatctgacgg cggccatctt ccacctgaca gacctctacg cgccttcgag cctgttccgg
      421 cgcatcggcc tgtggacggc gaccgccggt gtcttcttca acctggcgag ctggggcgtg
      481 cgctgggtga ccggctacga gcgcgagctt gcggtgatcg cgcggcaggg cggcgagatg
      541 ccgtgggcct tccgctacat cccgttcacg aacctctacg acatgagtct ggccttcgcc
      601 ttcggcgcgg ggctggcgac gctgttgatc gcgcaccggc agtcgttccg cttcctcggc
      661 gcgctctcgc tcccgctcgc cgcgctcatc ctcgtgctcg cgcgcttcat cggcggcgag
      721 atcgccaacc tgccggcggt gctcgattca tactggcgac ccatccacgt cggcacggcc
      781 tcgctctctt acggcgtcgc gctcgtctgc ttcgccgtcg cggtcgtcta cctgctgaaa
      841 gacggcgtca agaccgaggc gatggcgctg tggacaagcg gtttcgcgct cgcggtcttc
      901 gccgccgtca gccgcttctc ggtcttcgcg cccgcgacct tcggcaccta cagcgcctcg
      961 accatgttcg gcagctcgaa aatgtcgctg cctctccggg ccgacatccc ttacgtcggc
     1021 tggatgatcg tgctgagcgc agtgctgctc gcgggcgtcg tcgtcgcctt cgcgagatac
     1081 cttgcgaaga aagaccaacg agcgcgccgc tggggacaca gcctcctcaa gctctcgctc
     1141 gtcgcgcagg ccgccaccgt cgccgtgctc gtctcgcagt tgaaaagcat cacgggcgtc
     1201 gtcgcggcgt tagacccgcg gcagtacgcg cgcttcggcg cgtatctgct tgagcaggac
     1261 ggcatgagcg cggcgcaagt ctcgatgctc gcgccgtccc gactcgaaag cgtggccgcc
     1321 gacttcgtcc ggagccgcgg cgacgagctg ttcctgagcc tgcgcgccaa cccggtcgag
     1381 ctgtcggcgc tcatcacggc tgccgccgcc acactcttcg tcatcatctt cagcttccgc
     1441 accgagcggc tgcgcgcagg gttgccgtcg ttagagaaga tcgattcgct gatgtacaag
     1501 actgccggca tcaccttcgc gggcctcgcg ctcctgctca tcacgggcgc ggtctgggcc
     1561 aacgagtctt ggggccgcta ctggggctgg gacccgaagg aggtcggcgc gctcgtcgcg
     1621 tggctcacct acggcgcctt cctgcacgtg cgaatctcgc gcggctggac gggccgcccg
     1681 gcggcctact tcgcactggt cgcgttcctg ctcatcctct tcacctacct cggcgtcagc
     1741 tacctgctgc cgggcatgca ctcttacgct tgaggctgga ggttcgagga gcttccgtat
     1801 gtcactcgtc ctgacaggtg tgagccacaa gacagcgccg gtcgaggtgc gcgagcgcct
     1861 ggccttcgcc agaaacgcgc tgcctgaatc tttacgctcg ctcgtggatg gccgagtggt
     1921 gcgcgaggga atgattcttt cgacgtgcaa ccgcgtcgaa ctgctggttg acgctgaccc
     1981 cgcgcgcttg acggaagcgc gggcgcgagt gtccgaattc ttttcgacga gggcggaaga
     2041 cttggacgaa gagttgggcg ggcacgtcta tacgaaagaa gatgacgagg ccgtgcgcca
     2101 cctcctccgc gtcgcctcgt cgctcgactc gatgatgttg ggcgagccgc aaatcctcgg
     2161 ccaggtgcgg caggcttaca cggacgcctg cgacgcgggc accgcgggcc gcacgctcca
     2221 ccgcctgctg caccgcgcct tccacgccgc caagcgcgtg cggacggaga cgaacatcgc
     2281 ctcctcagcc gtctcggtct cattcgcggc ggtcgagctg gggcgcaaaa tcctcggcac
     2341 gctcgaaggg cgcacggtct tcctcgtcgg cgcgggcgag atggccgaac tcgccgcgcg
     2401 gcatctggct aaaaacggcg cctcgcgcat cctcttcgcc aaccgcacgc acgagacggc
     2461 gcgacgactc gccgcggagt tcggcggcga gtccgtccct tacgaacgct tcccggcggt
     2521 actcgccgag gccgacatcg tgatctgctc gaccggcgcg agctgccaca tcatcacgcc
     2581 gcggatggca cgcgcggcgc tgaagctgcg ccgcaaccgc ccctccttct tcatcgacat
     2641 cagcgtgccg cgcaacgtag acccgaaggt cggcgaggtc acgaacctct tcgtcttcga
     2701 cgtggacgac ctcgaagcgg tcgtcgcctc gaacctgcgc gagcgcgaac gcgaagccgc
     2761 gcgcgccgaa gagatcgtcg agacggaggc ggcggagttc ctgaaggcac ttcaccgcct
     2821 cgacatcggc ccgaccgtgc tcggtctcaa ggagaggctg cgggcgatcg cgcgggaaga
     2881 attccgccgg cggcggagca gtctcggcga cctcagccct gagcaggagc gcgccgtcga
     2941 gcaggtgctc gcggcgaccg tcaacaagat ttcacacccg ctcatccagc gcctgcgccg
     3001 ctccttcgac acgggcgacg acggggccgt ccgcgcatgg cgcgacagct tcaacctcgg
     3061 agacgagtcg gaaatcagag catcggtgca gacggcgctc gcccgcacat tcaaccttcg
     3121 aacggtcatc gcatgacagc ccggctcgtc gaaagattcg gcaaaaacgg ggcgcgtcgc
     3181 aacgggcgaa acaaggcaaa cgaagatcga ggtgagaaat gaagaaagat aacatcctgt
     3241 acggcatcat cggcgtgctc gtcggcttca tcgtcggctt tgccttcgcc aacacgacga
     3301 accagcgcgg ctacgcggcg cagagcacga cagccggaca gccgcagcag acggcgggct
     3361 tgccgcctga ccacccgccg attgacggcg caagcgctgg cccgaacgcg gcggcggtcg
     3421 gagcggcgat gaagctcgcc tcggagcagc ccggcaactt cgacgcgcag gtgaaggcgg
     3481 cggaagtcgc ctacgcggcg caccgctacg acgacgcgat aaaaattttc acccttgcca
     3541 accagctccg ccccgaacag ttcgacgtgc tcgtcggctt gggcaacacg aacttcgacg
     3601 ccgagcggtt cgaggaggct gagaagtggt atatcgccgc gctccgtcag cagcccgaca
     3661 acgtcaacgt ccgcacagac ctcggcctca ccttcttctt ccgcgagccg cgcgacgtgg
     3721 agcgcgccgt caaagagtac cgcgcctcgc tcgaacgtga ccccgcacac atcccgacac
     3781 ttcagaatct gaccgtcgcg ctcaccgcga agggcgacgc cgacgccgcc cgcgccgccc
     3841 tctccaagct cgaatccctc agcccgcaga accaggcact accgcgcctg cgcgccgacc
     3901 tcgaaaagct cgacgcgccg ggccggacgg caaccaacaa aacctcagcc gcagacatgg
     3961 gcggcaggta ggcaggaaag gaaacggagg ccactatgaa aagcgtaaag ctcggcgccg
     4021 gcttcacagt gtttttactc ttcttcgggg tggctgcgct cgaggcgttc caatcacgca
     4081 actgggtcaa gt
//
LOCUS       DBNGOH010000715         6710 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2490398, whole genome shotgun sequence.
ACCESSION   DBNGOH010000715 DBNGOH010000000
VERSION     DBNGOH010000715.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6710)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6710)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6710
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2490398"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..449
                     /locus_tag="AC159P_13870"
     CDS             <1..449
                     /locus_tag="AC159P_13870"
                     /inference="COORDINATES: protein motif:HMM:NF013429.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HIR3696583.1"
                     /translation="RSVDLGVPLGYHNHMNSLGEAPGEVDRILDAADPRYVKLELDVA
                     HYHQGGGDSAQAIRKYRDRLLFLHIKDVESPVPAAAGEQARAYRFVELGRGKVDLPSV
                     FKALKEVKFRGWAVVELDSVPDKTRTPKESALISKKYLEEKLGMKV"
     gene            complement(553..4167)
                     /locus_tag="AC159P_13875"
     CDS             complement(553..4167)
                     /locus_tag="AC159P_13875"
                     /inference="COORDINATES: protein motif:HMM:NF051698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HIR3696584.1"
                     /translation="MNSTDRFSPQPLPKFFLFACCLLVVLASAGQAAAQEFRGSIAGT
                     VNDSSGAAVPGATVTATNRATNTPQTTTTNEEGAYTLLYLTPGTYTLLVEATGFKRHL
                     SQGVEVRVGDKLVLDLRLETGGVQETVTVTSDPTLLQTSTASAGQVIDRRRISELPLS
                     DGNPFTLARLAAGATYTGELTFSRPFDNQGTSAIRADGAPGRNEFTLDGTPNMASGGG
                     QNGVGRVAFVPPADAVQEFKVETASFDAQQGHTAGANVNVTLKNGTNDLHGTLYEFVR
                     NDKLAANDFFLNRAGRPRTALRYNRYGATVGGPVLLPRFGEGGPGVWSGRDRTFFFFA
                     FEGLKDTFPEPTQFTVPTLAQRGGDFSALLSQGIQIYDPLTAGCGAPLVPPPAGQTTC
                     ASGVRVVRAAFPGNIIPATRISQIARNYLQFYPLPNQQGDALGRNNFISGQPRTDDFH
                     SESVRIDHTYTDKHKAFVRYTHNYRQEARSNWTGVLGGINPTGNFLFRINNGATYDHV
                     WTATPTTIVNARFGFSRFWEPNIRQHQGEFDPASLGFSARTAAFFGDASHLPLLDFPD
                     NTFSEIGNDMGAASSFNIYTFQPTVTKVFAAHSVRLGYDFRSYRENSYPATHAAGRYE
                     FASNFTRERDNSPALFGQEFAAFLLGQPTGGVIDRNASRANQTLYHGLFIHDDWKATP
                     KLTLNLGLRYELEGATTERFDRNVRGFDATAASPVEAAARAAYAASPIPQIAPANFLV
                     RGGLLFAGAGNRGFWEPDRNNFQPRVGFAYQWDSKTVVRGGWGLYMVPFVLDGIQQPG
                     FSQATNIVPTANTGLTFRANFADPFPDGVADPPGASQGLATFIGRAVEFVPVERKNGQ
                     AQRWSIGVQRELPGDWLVEASYVGNLGYDLEDEIELNSIPLQFLSTRTVRDQATIDFL
                     TANVTNPFRGLAPGTNHNNATIQRQQLLRPFPQFTTVTSRSYGGGSIYHSGQFRAERR
                     FSAGYTILASYTWSKFLQDVANFRDFGVEKTRVIALDDAPHRLVASGIYELPFGKGRR
                     WGRNWGKLTNALLGGFQVQGIWQYQSGRPINWGNVFYNGDPTQLRTNIDGATVDATFD
                     TSGFYFTDAAVQTNGVIDPAKQRADQRIRLANNVRYFPLRMSGFRGQPYHESHLSMIK
                     RVYFTESVNLELRGELINAFNHPQFNNPNVDPTSSNFGRATSMANLPRNIQLGAKLVF
                     "
     gene            4564..5433
                     /locus_tag="AC159P_13880"
     CDS             4564..5433
                     /locus_tag="AC159P_13880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HIR3696585.1"
                     /translation="MATFELSVFTDEITHDFGRAVEVAAKELGLGHVELRILWGKNIM
                     ALDAKEIAEARRLLEQRRLRVTSIAGPLFKVDWPGAPVSQFSQRDKFGADFTFAQQPE
                     VLERGLELARAFGTDRLRCFDFWRLDDPRPHRAAMDEELRKAAAKAAKRGVTLVMENE
                     YACNTATGAEAARTLAAVTARNFKLNWDPGNSAFRGEVPYPDAYELLPKDRIGHVHCK
                     DVERKPDGTFAWQAVGRGFIDFAGQFRALKRDGYRGAVVLETHWRGAGTPEESTKQSM
                     AGLKDALRKAGTM"
     gene            5577..>6710
                     /locus_tag="AC159P_13885"
     CDS             5577..>6710
                     /locus_tag="AC159P_13885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013666008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR3696586.1"
                     /translation="MSNKNNSRRDFIRTAALGAAGVALGPTAASYARVQGANSRVRVG
                     IVGFSDRARSSLIPAFMMSAKELNFDFVAVSDIWGRRREEGAEFLSKLVGHPIEKARN
                     NEELYERKDVDAVIISTADFQHALHGVEAVKAGRDAYVEKPLANTMEDARAILKAVDE
                     TKKIVQIGTQRRSGANYTRADEFIRSGKFGNIVMVEMSWNVNQPGRWRRPKLVKEISQ
                     QDVDWDRYLLNRREGGKKAEWDPRKYLEYRLFWPYSSGIPDQWMVHQIDTVHWFTGLP
                     RPRSVVANGGIYQWPDGRKNFDTMTAVFDYGPLGDVSKGFQVVYSSRMTNSAGGTKEL
                     YYSNAGMLNLDTNKITPEGGLTKRSAKSMNMPANLLPEMSLADA"
BASE COUNT         1120 a         2052 c         2449 g         1089 t
ORIGIN      
        1 agcgctcggt agacctcggc gtgccgctcg gctaccacaa ccacatgaac agtctgggcg
       61 aggcgcccgg cgaggtcgat cggattctcg acgccgcaga cccgcgctac gtgaagctcg
      121 aactcgacgt ggcccactac catcagggcg gcggcgactc ggcgcaggcc atcaggaagt
      181 accgcgaccg cctgctcttc ctccacatca aggacgtgga gagtcccgtg cccgcggcgg
      241 cgggcgagca ggcgcgcgcc taccgcttcg tcgaactcgg gcggggcaag gtcgatctgc
      301 cgtcggtctt caaggcgctc aaggaggtga aattccgcgg ctgggccgtc gtcgaactcg
      361 acagcgtgcc cgacaagacg cgcacgccga aagagtcggc gctcatcagc aagaagtatc
      421 tcgaagagaa gctggggatg aaagtttaag agggggcgaa gagggtaaaa ggggaagagg
      481 cgaagaggga gaagatgcca cttgcttcct cccttttcgc ctcttcccct tttaccctct
      541 tcgccccctg tcttagaaga cgagcttggc gccgagctgg atgttgcggg ggaggttggc
      601 catgctggtg gctctgccga agttggagct ggtcgggtca acgttcgggt tgttgaactg
      661 cgggtggttg aaggcgttga tcagctcgcc gcgcagttcg aggttgacgc tctcggtgaa
      721 gtagacgcgc ttgatcatcg agaggtgcga ctcgtggtag ggctggccgc ggaagccgga
      781 catgcggagc gggaagtagc ggacgttgtt ggcgaggcgg atgcgctggt cggcgcgctg
      841 cttggcgggg tcgatgacgc cgttggtctg caccgccgcg tcggtgaagt agaagccgct
      901 cgtgtcgaag gtggcgtcca cggtcgcgcc gtcgatgttg gtgcgcagtt gcgtggggtc
      961 gccgttgtag aagacgttgc cccagttgat cgggcgcccg ctctggtact gccagatgcc
     1021 ctgcacctgg aagccgccga gaagcgcgtt cgtgagcttg ccccagttgc ggccccagcg
     1081 gcggcccttg ccgaagggca gctcgtagat gccgctggcg acgaggcggt gcggcgcgtc
     1141 gtcgagcgcg atgacgcgcg tcttctccac gccgaagtcg cggaagttgg ccacgtcctg
     1201 aaggaacttc gaccacgtgt agctcgccag gatggtgtag cccgcggaga agcggcgctc
     1261 ggcgcggaac tggccggagt ggtagatgct gccgccgccg tacgagcgcg aggtgacggt
     1321 ggtgaactgc gggaaggggc gcaggagttg ttgccgctgg atggtcgcgt tgttgtggtt
     1381 ggtgccgggc gcgaggccgc ggaacgggtt ggtgacgttg gcggtgagga agtcaatcgt
     1441 cgcctggtcg cgcaccgtgc gggtcgagag gaattggagc gggatcgagt tcagctcgat
     1501 ctcgtcctca aggtcgtagc cgaggttgcc gacgtaggag gcctcgacga gccagtcgcc
     1561 cggcagctcg cgctggacgc cgattgacca gcgctgcgcc tgcccgttct tgcgctcgac
     1621 ggggacgaac tcgacggcgc gaccgatgaa ggtcgccaga ccctgcgagg cgccgggcgg
     1681 gtcggccacg ccgtcgggga acgggtccgc gaagttggcg cggaaggtca gtcccgtgtt
     1741 cgccgtcggc acgatgttgg tcgcctgcga gaagccgggc tgctggatgc cgtcgaggac
     1801 gaacggcacc atgtagaggc cccagccgcc gcgcacgacc gtcttcgagt cccactggta
     1861 ggcgaagccg acgcgcggct ggaagttgtt ccggtccggc tcccagaagc cgcggttgcc
     1921 ggcgcctgcg aagagcaggc cgccgcgcac gaggaagttc gcgggcgcga tctgcgggat
     1981 ggggctggcg gcgtaggcgg cgcgcgccgc cgcctcgacg gggctggcgg cggtcgcgtc
     2041 gaagccgcgg acgttgcggt cgaagcgctc ggtcgtcgcg ccttcgagtt catagcggag
     2101 gccgaggttg agcgtgagct tcggggtcgc cttccagtcg tcgtggatga agaggccgtg
     2161 gtagagcgtc tggttggcgc gcgaggcgtt gcggtcgatg acgcccccgg tgggctggcc
     2221 gagcaggaag gcggcgaact cctgaccgaa gagcgcgggc gagttgtcgc gctcgcgcgt
     2281 gaagttcgag gcgaactcgt agcgcccggc ggcgtgcgtc gccgggtacg agttctcgcg
     2341 gtaggagcgg aagtcgtagc cgaggcggac ggagtgcgcg gcgaagacct tcgtcaccgt
     2401 cggctggaag gtgtagatgt tgaaggacga ggcggcgccc atgtcgttgc cgatctcgct
     2461 gaaggtgttg tccggaaagt ccaggagcgg caggtgcgag gcgtcgccga agaacgcggc
     2521 ggtgcgcgcg gagaagccca aagaagcggg gtcgaactcg ccctgatgct ggcggatgtt
     2581 cggctcccag aagcgcgaga agccgaagcg cgcgttgacg atggtggtcg gcgtggccgt
     2641 ccagacgtgg tcgtaggtcg cgccgttgtt gatgcggaag aggaagttgc cggtcgggtt
     2701 gatgccgccc agcacgcccg tccagttgct ccgcgcctcc tgccggtagt tgtgcgtgta
     2761 gcggacgaac gccttgtgct tgtcggtgta ggtgtggtcg atgcgcaccg actcggagtg
     2821 gaagtcgtcg gtgcgcggct ggccgctgat gaagttgttg cgcccgagcg cgtcgccctg
     2881 ttggttgggc agcgggtaga actgcaagta gttgcgcgcg atctggctga tgcgcgtcgc
     2941 agggatgatg ttgccgggga aggccgcgcg cacgacgcgc acgcccgagg cgcacgtcgt
     3001 ctggcccgcg ggcggcggga cgagcggcgc gccgcacccc gcggtcagcg ggtcgtaaat
     3061 ctggatgccc tgcgagagga gcgccgagaa gtcgccgccg cgctgcgcga gcgtgggcac
     3121 ggtgaactgc gtcggctcgg ggaaggtgtc cttgaggccc tcgaaggcga agaagaagaa
     3181 ggtgcggtcg cgcccgctcc acacgccggg gccgccctcg ccgaagcgcg gcagcagcac
     3241 ggggccgccg accgtcgcgc cgtagcggtt gtagcggagc gccgtgcgcg ggcggcccgc
     3301 gcggttgagg aagaagtcgt tggccgcgag cttgtcgttg cggacgaact cgtagagcgt
     3361 gccgtgcagg tcgttggtgc cgttcttgag cgtgacgttg acgttcgccc cggcggtgtg
     3421 cccctgctgc gcgtcgaagc tcgcggtctc gaccttgaac tcctgcacgg cgtccgccgg
     3481 cggcacgaag gcgacgcgcc cgacgccgtt ctgcccgccg ccgctcgcca tgttcggcgt
     3541 gccgtcgagc gtgaactcgt tgcggcccgg cgcgccgtcg gcgcggatgg ccgaggtgcc
     3601 ttgattgtcg aacgggcgcg agaacgtcag ctcgcccgtg taggtggcgc ccgccgcgag
     3661 ccgcgccagc gtgaacgggt tgccgtccga gagcggcagc tccgagatgc ggcggcggtc
     3721 gatgacctga ccggcggagg ccgtcgaggt ctgaaggagc gtcgggtcgc tcgtcacggt
     3781 gacggtctcc tgcacgccgc cggtttcgag ccggaggtcg agcacgagct tgtcgccgac
     3841 gcgcacctcg acgccctggc tgaggtgcct cttgaagccg gtcgcctcga ccagcagcgt
     3901 gtacgtgccg ggcgtcaggt agagcagcgt gtaggcaccc tcctcgttgg tcgtcgtcgt
     3961 ctgcggcgtg ttcgtcgccc ggttggtggc cgtgacggtc gcgcccggca ccgccgcgcc
     4021 cgacgagtcg ttgaccgtgc ccgcgatgga gccgcgaaac tcctgcgccg ccgcctggcc
     4081 cgccgaggcc agcacgacta acaggcagca cgcgaacagg aaaaatttgg ggagaggttg
     4141 cggcgagaat ctgtcggtcg agttcatata tcctccgagt cggctgttgg gaagggctgg
     4201 cttcgtcggg ggcggattcc gcctacgagc aggacggcga cgttggcccg aagggcgcgc
     4261 gaatcgtgtg cggttcttcg tccccgaacg gaacaggaag ctgggcggcg gaataatatt
     4321 tgttaccgat aacaaaatca agtattatgt tgccacgcac cggcgacggg gccgcggcgg
     4381 cttgccgctt cgactcttgg gaaaagaagg gtcttgactg tcgcggcccc tagtgttagg
     4441 aatctcgtcg cacccagttt tcacacggca acaagagagg gccggcgagt ccgccgcgcc
     4501 gagacgcccc ggcctacctt cgccgggcga cacgggaaag ttcatcttcg accgaatcat
     4561 tcgatggcga cattcgaact ctctgttttc acggacgaga tcacgcacga cttcgggcgc
     4621 gcggtcgagg tggccgcgaa ggagctgggc ctcggccacg tcgagctgcg catcctgtgg
     4681 ggcaagaaca tcatggcgct cgacgccaag gagatcgccg aggcgcggcg gctgctcgaa
     4741 cagcggcggc tgcgcgtgac ctcgattgcc gggccgctct tcaaagtcga ttggccgggc
     4801 gcgcccgtct cgcagttcag ccagcgcgac aagttcggcg ccgacttcac cttcgcgcag
     4861 caaccggagg tcctggagcg cgggctggag ctggcgcgcg ccttcggcac ggaccgcctg
     4921 cgctgcttcg acttctggcg gctcgacgac ccgcgaccgc accgcgcggc gatggacgaa
     4981 gagctgcgca aggcggcggc caaggcggcg aagcgcggcg tcacgctcgt catggagaac
     5041 gagtacgcct gcaacacggc gaccggcgcc gaggccgcgc gcacgctcgc cgccgtcacc
     5101 gcgcgcaact tcaaactcaa ctgggacccc ggcaactccg ccttccgcgg cgaggtgccg
     5161 tacccggacg cctacgaact gctgcccaaa gaccgcatcg gccacgtcca ctgcaaggac
     5221 gtggagcgca aacccgacgg caccttcgcg tggcaggcgg tgggtcgcgg cttcatcgac
     5281 ttcgccgggc agttccgcgc gctcaagcgc gacggctacc gcggcgccgt cgtcctcgaa
     5341 acgcactggc gcggcgcggg cacgcccgaa gagtcaacga agcagagcat ggccgggctg
     5401 aaagacgcgc tgcggaaagc gggcacgatg tgaaagaagc tgttagctat tagctgttag
     5461 ctgttagctt ccggcttaac cactgacggt tgatgggagc ggcgcttgta tcaatcgtca
     5521 gtcatccaat cagacagcta atagctaaca gctaatagct aatagcggag agcgtcatgt
     5581 cgaacaaaaa caattcgcgc agagacttta tcaggaccgc ggcgctcggc gccgcgggcg
     5641 tcgcgctcgg cccgacggcg gcgagctacg cgagggtgca gggggccaac tcgcgcgtgc
     5701 gtgtcggcat cgtcggcttc tccgaccgcg cccgttcgtc tctgattccg gccttcatga
     5761 tgagcgccaa agaactcaac ttcgacttcg tggccgtctc cgacatctgg ggccgccgcc
     5821 gcgaagaggg cgccgagttt ctgtcgaagc tcgtcggcca tccaatcgag aaggcgcgca
     5881 acaacgagga gctgtacgaa cgcaaggacg tggacgccgt catcatctcg accgccgact
     5941 tccagcacgc gctgcacggc gtcgaggcgg tgaaggccgg acgtgacgcc tacgtcgaaa
     6001 agccgctcgc caacacgatg gaggacgcgc gcgcgatcct gaaagcggtg gacgagacga
     6061 agaagatcgt ccagatcggc acgcagcgcc ggagcggcgc gaactacacg cgcgccgacg
     6121 agttcatccg ctcgggcaag ttcggcaaca tcgtgatggt cgagatgtcg tggaacgtga
     6181 accagcccgg tcgctggcgg cgcccgaaac tcgtcaaaga gatttcgcag caggacgtgg
     6241 actgggatcg ctacctgttg aaccggcgcg agggcggcaa gaaggccgag tgggacccgc
     6301 gcaagtatct tgagtaccgc ctgttctggc cctactcgtc gggcatcccc gaccagtgga
     6361 tggtgcacca gatcgacacg gtgcactggt tcacgggcct gccgcgcccg cgctcggtgg
     6421 tcgccaacgg cggcatctac cagtggcccg acgggcgcaa gaacttcgac acgatgacgg
     6481 cggtcttcga ctacggcccg ctcggcgacg tgtcgaaggg cttccaggtc gtctactctt
     6541 cgcgcatgac gaactcggcg ggcgggacga aggagctgta ctactcgaac gcagggatgc
     6601 tcaacctcga caccaacaag atcacgcccg agggcggcct gaccaagcgg tccgccaagt
     6661 cgatgaacat gccggcgaac ctgctcccgg agatgtctct cgccgacgcg
//
LOCUS       DBNGOH010000716         3242 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3222711, whole genome shotgun sequence.
ACCESSION   DBNGOH010000716 DBNGOH010000000
VERSION     DBNGOH010000716.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3242)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3242)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3242
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3222711"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(210..1586)
                     /locus_tag="AC159P_13890"
     CDS             complement(210..1586)
                     /locus_tag="AC159P_13890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696587.1"
                     /translation="MGKEQKLEHLEKVLHSRTLQNSESLKAFLRFVVEKAVDDQEAQL
                     KEYTIATEVFGRDSDYDPRIDSVVRVQAGRLRTKLQEYYAGEGKADEVVIDLPKGHYH
                     PVFNYARAAHTTQEAIHTTSPATSEAATNGHPHAAAPHEHAAVETAPAGLSERTLRLF
                     LVGIIVALCFALLALYYNNRELRQSQTVVDSSPFNAEEFKAVWGLFVSDPEPPLLILS
                     NPAVYRFLNRADPAAATARALHLTPEQVRTTTETPEFREQQLPIDETQPQLLLSVGMY
                     TGMGEAIGLYRLSTLFRSANRDILLRQSRHANAADLKYRNVILLGSVYVNEWSRKLPV
                     SESFNYTNAATIENRLPLAGEEKEYRPQFDERTGELRTDYALITVKPNVSGDHAVMTL
                     AGLYSEGTEAAAEFITTKNHLAVLAQRLKDAGGQGAPPKYYQALLKVGVENGTPTTIT
                     LVALRPIG"
     gene            2276..>3242
                     /locus_tag="AC159P_13895"
     CDS             2276..>3242
                     /locus_tag="AC159P_13895"
                     /inference="COORDINATES: protein motif:HMM:NF019335.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HIR3696588.1"
                     /translation="MRSSGLWLLSLASCLLLLLAPLAHAQKSSGQITGEVVDQNGAAL
                     PDTKVTVTQVGTNLVREVNTNQDGIYSVPDLPIGTYRVSATQGGFKETVVEGVAVNVS
                     TTTRQDFTMQIGAVGERVDIIANEVQIQQETGAVGDVVTGEQVRELPLNGRSFVQLTQ
                     LQPGVSPASNFDSKNKGLFSGVDFSVNGNTTQSNLFLIDGANNNDTGSNRTILLYPSI
                     EAIGEFKMLRNSFSPEYGQASGAVISIATRGGENEFHGSLFYFGRNDKLNAADYFANR
                     VGGTKDKLRRNDFGGSIGGPIIKDRLFFFYSQEWNKEIRGQTRFGS"
BASE COUNT          566 a          991 c         1076 g          609 t
ORIGIN      
        1 tccttctcct ggccgcgctc gatgtccgac attcccgtca acgtcggcga ccggccttac
       61 gacccgctct tcaagtacgg cttcggcctc acgtattaaa aaaaagggga aaaggttaaa
      121 aggggaagag gcgaaaaggg aagaagcgtc acgtgggccg cgctctccct tttcgcctct
      181 tcccctttta accttttccc cctccttctt tatccgatgg ggcggagcgc gacgagtgtg
      241 atggtggtgg gcgtgccgtt ctcgacgccg accttgagga gcgcctgata gtatttcggc
      301 ggcgcgccct ggccgcccgc gtccttcagt cgctgggcga ggacggcgag gtggttcttg
      361 gtggtgatga actcggcggc ggcttcggtg ccttcgctgt agaggccggc gagggtcatc
      421 acggcgtggt cgccggagac gttgggcttg acggtgatga gcgcgtagtc cgtcctaagc
      481 tcgcccgtcc gctcgtcgaa ctgcggcctg tactccttct cctcgccggc cagcggcagg
      541 cggttctcga tggtggcggc gttcgtgtag ttgaagctct cgctgacggg gagtttccgc
      601 gaccactcgt tgacgtagac gctccccagc aggatgacgt tgcggtactt gaggtcggcg
      661 gcgttggcgt ggcggctctg ccgcaagagg atgtcgcggt tcgcggagcg gaagagcgtg
      721 ctcaggcggt agaggccgat ggcctcgccc atgcccgtgt acatgccgac cgagagcagt
      781 agttgcggct gcgtctcgtc aatcgggagt tgctgctccc tgaactccgg cgtctcggtc
      841 gtggtgcgca cctgctcggg cgtgaggtgg agcgcgcgcg ccgtggcggc ggcggggtct
      901 gcgcggttga ggaagcggta gaccgccggg ttgctcagga tgagcagcgg aggctccggg
      961 tcgctgacga acaggcccca gacggccttg aactcctcgg cgttgaaggg cgaagagtca
     1021 accactgtct ggctctgtcg cagctcgcgg ttattgtagt agagcgcgag cagcgcgaag
     1081 cagagggcga cgatgatgcc gacgaggaag aggcgcagcg tcctttcgga gaggccggcg
     1141 ggcgccgtct cgaccgccgc gtgttcgtgc ggcgcggcgg cgtgcgggtg gccgttcgtc
     1201 gccgcctccg aggtcgcggg cgacgtggtg tggatcgcct cctgcgtcgt gtgcgccgcg
     1261 cgcgcgtagt tgaagacggg gtggtagtgg cctttgggca ggtcgatgac gacctcgtcg
     1321 gccttgcctt cgcccgcgta atactcctgg agcttcgtcc gtagccgtcc ggcctgcacg
     1381 cgcacgaccg agtcgatgcg cgggtcgtag tcgctgtcgc gaccgaagac ctcggtcgcg
     1441 atggtgtact ctttgagctg cgcctcctgg tcgtccaccg ccttctcgac gacgaagcgg
     1501 aggaacgcct tgaggctctc cgagttttgc agcgtgcggc tgtggagcac cttctccagg
     1561 tgctccagtt tttgttcctt gcccaactcc atacgcttac aacgaacctg ttcagagcgc
     1621 ccgagaatgt agacggcgcc gcgaggcggc gtccccttgg cacgtgcgag ttgacaatag
     1681 ttgttcggta actgcgaagg atggaaaccc ttgctgacgg cgaaccggat tataagccca
     1741 tgtcgtgctt ttcaacaaac gagactgcgc gccgaccgta aggaaagggc gcggggtgtg
     1801 ccgcgcgggc cgacgattta cgcccgtatt tcgccgatgt aacgcgttac accggggctg
     1861 taacgcgcgt ttttcccaaa cctttcctaa accgccgaca atatcacctg tccccgcgcg
     1921 gagtatctaa ccgtgtcata acgggcggta acttttaggt caatcgaaag ttggatatag
     1981 tcatcaacgg tcttaatccg cccgcgcgtc gtgctagtag cgaaaacttc aggtcgttcg
     2041 aacgtcgatg cggcgtcgcc gggacgagtt agacggccag ccctgcccac aagccagttt
     2101 gctcgaatcg ctgttgtgac gatctcgccg cccttgccaa agagcgcgcc acttggaatc
     2161 cgttgtcacg ggatcaaccc gcttcgcaga aagtcatctg gaggagacgc ccaaaatgag
     2221 taaggcagaa tcgtgccgga gtctggcacc cgagagaaaa cagacgcgac gcggcgtgag
     2281 atcatccggc ctgtggctgt tgtcactggc gtcctgcctg ctcctgctgc tggcgcctct
     2341 ggctcacgcg cagaagagtt cggggcagat caccggcgag gtcgtggatc agaacggcgc
     2401 ggcgttgccc gacacgaagg tgacggtcac ccaggtcggg accaacctgg tgcgcgaggt
     2461 gaacaccaat caggacggca tctactcggt gcccgacctg cccatcggca cgtaccgcgt
     2521 ctcggcaacg cagggcgggt tcaaggagac ggtcgtcgag ggcgtcgcgg tcaacgtctc
     2581 gaccaccacg cggcaggact tcacgatgca gatcggcgcg gtcggcgagc gcgtggacat
     2641 catcgccaac gaggtgcaga ttcagcagga gaccggcgcc gtgggcgatg tcgtcacggg
     2701 cgagcaggtg cgggaactgc cgctgaacgg gcgcagcttc gtccagctca cgcagctcca
     2761 gccgggcgtc tcgcccgcga gcaacttcga cagcaagaac aaggggctgt tctccggcgt
     2821 ggacttctcc gtcaacggca acacgacgca gagcaacctg ttcctcattg acggcgccaa
     2881 caacaacgac accggctcga accgcaccat cctgctctac ccgtccatcg aggccatcgg
     2941 cgagttcaag atgctgcgca acagcttcag ccccgagtac gggcaggcct cgggcgcggt
     3001 catctcgatt gcgacgcgcg gcggcgagaa cgagttccac ggcagcctct tctacttcgg
     3061 gcgcaacgac aagctgaacg ccgccgacta cttcgccaac cgggtgggcg gcacgaagga
     3121 caagctccgg cgcaacgact tcggcggctc gatcggcggc ccgatcatca aagaccggct
     3181 cttcttcttc tactcgcagg agtggaacaa ggagattcgc ggccagacgc gcttcggcag
     3241 cg
//
LOCUS       DBNGOH010000717         3408 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1390118, whole genome shotgun sequence.
ACCESSION   DBNGOH010000717 DBNGOH010000000
VERSION     DBNGOH010000717.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3408)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3408)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3408
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1390118"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..496
                     /locus_tag="AC159P_13900"
     CDS             <1..496
                     /locus_tag="AC159P_13900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HIR3696589.1"
                     /translation="LRGGCEMGRAVLKALNFRTGFTHMEWYKKADGEIVFGEIAARPP
                     GARSVDVMNYASDVDLFTGWAEAVVHGRFTQPVERRHNAVIIFKRAQGAGRIRRIEGL
                     ERILGHFGEHVACIDLLAPGEHRRNWVQTLISDGYLVVRHPSLPRALEMADAVGTDLQ
                     LYAG"
     gene            609..1478
                     /locus_tag="AC159P_13905"
     CDS             609..1478
                     /locus_tag="AC159P_13905"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012958.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR3696590.1"
                     /translation="MTTSTDTMPGHDGSEAITNSVTTDGMEEDRGKYELHARFHSELL
                     VKDRDVIVYLPPGYDAEGARRFPVLYLQDGQNLFDGATAFVYGQHWRVGETADALVRA
                     RAVEPLVIVGIYNAGEKRVDEYTPTHDRRRQIGGQADIYGRFLVEELKPFIDARYRTL
                     TDAANTGLGGSSLGGLVSLHLGIKHPQVFGKLAVLSPSIWWDRRVILRKVRTLEAKAA
                     LRIWIDVGTEEGGYAVKDAAMLRDALVRRGWVEDVDLKYLEAVGAQHNELAWAERVGP
                     VLEFLFPHGAQGG"
     gene            1475..2407
                     /locus_tag="AC159P_13910"
     CDS             1475..2407
                     /locus_tag="AC159P_13910"
                     /inference="COORDINATES: protein motif:HMM:NF013702.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HIR3696591.1"
                     /translation="MSGPVTSTPDARDDSPALLLEPAREELAALSRMEWFAFRLTRRM
                     NRGAWKRLWTFCQSTVGAGWIHLATYNLMRVYGLEHVERVSHERPLLLAANHRSFFDM
                     YAVSSVLFRRTRWRKKLFFPVRGRFFYDSVGGLLVNFVMGWWSMFPPFFTGGDSPQPE
                     KREFDKFTMRLLTELCREGAGNVVGFHPEGKRNKGEDPYTYLRPQPGIGKLVKDARPQ
                     VVPVFIAGLGNDLFRQVLGNWRGGQPVRIHFGPPLDLSDFYDRREHLRTYKEIGEFVM
                     SKIAELGEQDRAIRQLEAAKGAIPTSPSTFSTNS"
     gene            2473..>3408
                     /locus_tag="AC159P_13915"
     CDS             2473..>3408
                     /locus_tag="AC159P_13915"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol/lipid kinase family protein"
                     /protein_id="HIR3696592.1"
                     /translation="MSLPLIIVNPASADGATGDTWPGIAGEVRRHFGPFNVAFTETGG
                     DAARIARREAEAGRAFVIACGGDGTINEAANGVLESGADCEFGILPSGTGGDFRRTLR
                     IPARASDAALALSRAATRRMDVGRVTYAGRDGAVAARYFLNVASFGMGGEVIKRVKDN
                     RWLPRGGARLLGGKASFAAAALGAALEYENPSVVVRLDDNPERRLVVTNFCVANARYF
                     GGGMKIAPEAKIDDGLLDVVCIGDMDRLAVLWNSYRVYLGTHLGLRDVYHARAKTVTA
                     RADPNVDVELEVDGELVGKLPATFEILPRALKIRCG"
BASE COUNT          587 a         1197 c         1103 g          521 t
ORIGIN      
        1 gcttcggggc ggttgcgaga tggggcgcgc ggtgctgaag gctctgaact tccgcacggg
       61 cttcacgcac atggagtggt acaagaaggc ggacggcgag atcgtcttcg gcgagatcgc
      121 ggcccgcccg ccgggcgcgc gctcggtgga cgtgatgaac tacgcctcgg acgtagacct
      181 cttcaccggc tgggccgagg cggtcgtgca cggacggttc acgcagccgg tcgaacgacg
      241 ccacaacgcc gtcatcatct tcaagcgcgc gcaaggcgcg gggcgcatcc gccgcatcga
      301 ggggctggaa cgcatcctcg ggcacttcgg cgagcacgtc gcctgcatcg acctgctcgc
      361 gcccggcgag caccggcgca actgggtgca gacgctcatc tccgacggct acctcgtcgt
      421 ccgccacccg agcctgccgc gcgcgctgga gatggccgac gccgtcggca ccgatctgca
      481 actctacgcg ggctgaaaat cgacgctttt tcggaaggcg cggcgcgcgg attcgggtta
      541 aaatgaacgc gcgttcaggg taaaacgaac gcacgccgga ctcgatcgag gggattcaca
      601 aacgcgtaat gacgacatcg acggacacca tgcccggaca tgacggctct gaagcgatca
      661 ccaattcggt cacgaccgac gggatggaag aggatagagg gaagtacgaa cttcacgcgc
      721 gcttccactc cgagctgttg gtgaaggaca gggacgtgat cgtgtacctg ccgcccggct
      781 acgacgccga aggcgcgcgg cgcttccccg tcctctacct gcaagacggg cagaacctct
      841 tcgacggcgc gaccgccttc gtctacgggc agcactggcg cgtgggcgag accgccgacg
      901 ccctcgtccg cgcgcgcgcg gtcgagccgc tcgtcatcgt cggcatctac aacgcgggcg
      961 agaagcgcgt ggacgagtac acgccgacgc acgaccgccg ccggcagatc ggcgggcagg
     1021 cggacatcta cgggcgcttc ctcgtcgaag agctgaagcc gttcatcgac gcgcgctacc
     1081 gcacgctcac ggatgcggcg aacaccggcc tcggcggctc cagcctcggc gggctggtct
     1141 cgctccacct cggcatcaag cacccgcaag tcttcggcaa gctcgccgtg ctctcgccct
     1201 cgatctggtg ggatcggcgc gtcatcctgc gcaaggtgcg caccctcgaa gcgaaggcgg
     1261 cgctgcgcat ctggatcgac gtggggacgg aagagggcgg ctacgccgtg aaggacgcgg
     1321 cgatgctacg cgacgcgctc gtccggcgcg gctgggtcga ggacgtggac ttgaagtacc
     1381 tcgaagccgt gggcgcgcag cacaacgagc tggcgtgggc cgagcgggtc gggccggtgc
     1441 tcgaattcct gttcccgcac ggggcgcaag gcggatgagc ggacccgtca catcgacccc
     1501 ggacgcgcgc gacgactcgc cggcgctcct gcttgagccg gcgcgcgaag agctggcggc
     1561 gctctcgcgc atggagtggt tcgccttccg cctcacgcgg cggatgaatc gcggcgcgtg
     1621 gaagcgcctc tggactttct gccagagcac ggtcggcgcg ggctggatac atctggcgac
     1681 ttacaacctg atgcgcgtct acggcctcga acacgtcgag cgcgtctcgc acgaaagacc
     1741 gctcctcttg gccgccaacc accgctcgtt cttcgacatg tacgccgttt cttccgtcct
     1801 cttccggcgg acgcgctggc gcaagaaact cttcttcccc gtccgcggtc gcttcttcta
     1861 cgactctgtg ggcggtctgc tcgtcaactt cgtcatgggc tggtggtcga tgtttccgcc
     1921 cttcttcacc ggcggcgaca gcccccaacc agagaagcgc gagttcgaca agttcacgat
     1981 gcgcctgctg accgagctgt gccgcgaggg cgcgggcaac gtcgtcggct tccaccccga
     2041 aggcaagcgc aacaagggcg aagaccctta cacctacctg cgaccgcagc ccggcatcgg
     2101 caaactcgtc aaggacgcgc gcccgcaggt cgtgcccgtc ttcatcgccg gactcggcaa
     2161 cgacctcttc cggcaggtgc tcggcaactg gcgcggcggc cagcccgtcc gcatccactt
     2221 cggccccccg ctcgacctct ccgacttcta cgaccggcgc gaacacctgc gaacctacaa
     2281 agagatcggc gaattcgtca tgtccaaaat cgccgaactc ggcgagcaag accgcgccat
     2341 ccgacaactc gaagcggcca aaggagccat cccaacgtcg ccttcaacct tcagcaccaa
     2401 ctcatgaaag ccgcgaaccg taaaccgtaa accgagaacc gaacaacacg catccgcaat
     2461 ccgcaatcgc gaatgtcgct gcctctcatc atcgtcaacc ctgcctccgc cgacggcgcg
     2521 accggcgaca cgtggcccgg catcgccggc gaggtgcggc gacacttcgg ccccttcaat
     2581 gtcgccttca ctgagacggg cggcgacgcc gcacgcatcg cgcggcgcga ggccgaggcg
     2641 gggcgcgcgt ttgtcatcgc ctgcggcggc gacggcacca tcaacgaggc ggcgaacggc
     2701 gtccttgagt ccggcgccga ctgcgagttc ggcatcctgc cgagcggcac cggcggcgac
     2761 ttccgccgca cgctccgcat ccccgcacgc gcgagcgacg ccgcgctcgc actcagccgc
     2821 gccgccacgc gccgcatgga cgtgggccgc gtcacctacg cgggccgcga cggcgccgtc
     2881 gcggcgagat actttctgaa cgtcgcgtcg ttcgggatgg gcggcgaggt catcaagcgc
     2941 gtgaaggaca accgctggct cccgcgcggc ggcgccagac tgctcggcgg caaagcctct
     3001 ttcgcggcgg cggcgctcgg cgcggcgctc gaatacgaaa acccttcggt cgtcgtccgc
     3061 ctcgacgata accccgagcg ccgcctcgtc gtcaccaact tctgcgtggc caacgcgcgc
     3121 tacttcggcg gcggcatgaa gatcgcgccc gaggcgaaga tcgacgacgg cctcctcgat
     3181 gtcgtctgca tcggcgacat ggacaggctc gccgtcctct ggaactccta ccgcgtctac
     3241 ctcggcacgc acctcggcct gcgcgacgtt tatcacgcac gcgcaaagac cgtcacggct
     3301 cgcgccgatc ccaacgtcga tgtcgagctt gaggttgatg gagagttggt ggggaagctg
     3361 cccgcgactt tcgaaatcct gccgcgggcg ttgaagattc ggtgcggc
//
LOCUS       DBNGOH010000718         2827 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2856275, whole genome shotgun sequence.
ACCESSION   DBNGOH010000718 DBNGOH010000000
VERSION     DBNGOH010000718.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2827)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2827)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2827
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2856275"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            143..1723
                     /locus_tag="AC159P_13920"
     CDS             143..1723
                     /locus_tag="AC159P_13920"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF023418.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase TgpA family protein"
                     /protein_id="HIR3696593.1"
                     /translation="MAAGKASSGYVGFSDQMTLGDIGRLQESNQLVMRVRVEGAPAQG
                     RHLFWRGVALEQFDGRRWRRSPEDDDRRPVVYERNLFRLGTTEDVRRLTTQTFFIEPI
                     DTPVLFAAPRAVAVQGELPFVRRDSADGLTSRAHFEERITYRVYSDTIEPDEARLRTD
                     RGRLPVEPTPNLRRPVDHYLELPPQLDTRIASLAALVIMESGARNIYDAARAVEAHLG
                     RNQYGGQYSYTLDMRASGADPVADFLFNVRAGHCEYFATAMAVMLRTQGFPTRVVNGF
                     QSGEYNDAADAYTVRQADAHSWVEVYFPGTDAWVTFDPTPVAGRPSGAADASGLRAQV
                     RKYTDALELFWIQYVVAYDKQGQRTLARSLRERLNEYRGGAASAAGSLTATLASWWNG
                     MKTKTEQLGFVDVQTAAPVFVVAALALIVFAWRKKLFKLGGGGEKANAAPAKAADIDF
                     YKRMVKALEARGQRRAADQTPLEFASTLGTPEALIVTDAYHRVRYGGQGLNADEAREV
                     DECLRRIETSAAEVGLRK"
     gene            1912..>2827
                     /locus_tag="AC159P_13925"
     CDS             1912..>2827
                     /locus_tag="AC159P_13925"
                     /EC_number="2.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035596 - methylthiotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MiaB/RimO family radical SAM
                     methylthiotransferase"
                     /protein_id="HIR3696594.1"
                     /translation="MKKVGFISLGCPKNLVDSEVMMGQLKAKGYEITSDAAEADTLVV
                     NTCGFIDAAKKESVDAILEAARLKSEGNCRRVVVAGCLVERYRDELRAELPEVDAFIG
                     TNQINDITTVCDEKVNTRQLPVLPLGNQSATYLYDESSPRVLATPGHFAYVKIAEGCD
                     RPCAFCFIPQMRGHFRSRRFGSVIAEAQALAARGVKELILVAQDSSRYGEDLGERDAL
                     AHLMRELCRLEGVEWVRVMYTYPTHISDAFLDALAEEPKAVKYLDMPLQHASANVLKL
                     MKRGGNRASLERLIEKVRRRVPGVAVRTT"
BASE COUNT          506 a          961 c          952 g          408 t
ORIGIN      
        1 cagcccgttg ggcagcgtgt gtcgcacccg cacaacctaa caggcgggcg gcgccgcgca
       61 ccccggccgt gaaaaagagt cgttcagccg acgggaagcg agcgggctcc gcctctggcg
      121 ctcgggcagc agcgccctgt cgatggccgc gggcaaggcg tcgagcggct acgtcggctt
      181 ctccgaccag atgacgctcg gcgacatcgg gcgcttgcag gagagcaacc agctcgtgat
      241 gcgcgtccgc gtcgagggcg cgcccgcgca aggccgtcac ctcttctggc gcggcgtggc
      301 gctcgaacag ttcgacggtc gcaggtggcg acgctcgccg gaagacgacg acaggcgacc
      361 ggtcgtgtac gagcggaacc tgttccgcct cggcacgacc gaggacgtgc ggcggctgac
      421 gacgcagacc ttcttcatcg agccgataga cacgcccgtg ctcttcgccg cgccgcgcgc
      481 cgtcgccgtg cagggcgagc tgcccttcgt ccggcgcgac agcgccgacg gcctcacctc
      541 gcgcgcgcac ttcgaggagc gcatcaccta ccgcgtctac tccgacacca tcgagccgga
      601 cgaggcgcgg ctgcgcacag accgcggtcg cctgcccgtc gagccgacgc cgaacctgcg
      661 ccgccccgtc gatcactatc tcgaactccc cccgcagctc gacacgcgca tcgccagcct
      721 cgccgccctc gtcatcatgg agtccggcgc gcgcaacatc tacgacgcgg cccgcgccgt
      781 cgaggcgcac ctcggtcgca accagtacgg cggccagtac agctacacgc tcgacatgcg
      841 cgcctccggc gcggaccctg tggccgactt cctcttcaac gtgcgcgcgg gccactgcga
      901 atacttcgcc accgcgatgg ccgtcatgct ccgcacgcag ggcttcccga cgcgcgtcgt
      961 caacggcttc cagtcgggcg aatacaacga cgcggcggac gcctacaccg tccggcaggc
     1021 cgacgcccac tcgtgggtcg aggtctactt ccccggcacg gacgcgtggg tgacgttcga
     1081 cccgacgccc gtcgcgggcc gcccgagcgg cgcggcggac gcctcgggcc tgcgcgcgca
     1141 ggtgcgaaag tacaccgacg cgctggagct gttttggatt caatacgtcg tcgcctatga
     1201 taagcagggg cagcgcacgc tggcgcgctc gttgcgcgag aggctgaacg agtaccgcgg
     1261 cggcgccgcg agcgcggcgg gaagtctgac ggcgaccctc gcgtcgtggt ggaacggcat
     1321 gaagacgaag accgagcagc tgggcttcgt tgacgtgcag acggcggcgc cggtgttcgt
     1381 cgtcgcggcg ctcgcgctca tcgtcttcgc gtggcggaag aagctgttca agctaggggg
     1441 cggcggcgag aaggcgaacg ccgcgcccgc gaaagccgcg gacatcgatt tctacaaacg
     1501 catggtgaag gcgctcgaag cgcgcgggca gcgtcgcgcc gccgaccaga cgccgctcga
     1561 attcgcctcc accctcggca cgcccgaggc gctcatcgtg acggacgcct accaccgcgt
     1621 acgctacggc gggcagggtt tgaacgccga cgaagcgagg gaagttgacg agtgtctgcg
     1681 gcgcatcgaa acgagcgcgg cggaagtcgg attgcgtaaa tgatactgag agcgcggcgc
     1741 aatactgtaa aggcagggca agtccgcggc ccgcggtcgg attatcttca acgttgaatc
     1801 aaaagtgagt cggcgggcag ggacaagctc tgcccctacg gttatcacgg acggagaagg
     1861 gtttccaaaa cgaacttcaa ggtgtcggca agaaagatcg agtaattatt catgaagaaa
     1921 gtcggattca tcagtctggg ctgtccgaag aacctcgtcg atagcgaggt gatgatgggc
     1981 cagctcaaag cgaagggtta cgaaatcacg agcgacgccg cggaggccga tacgctggtg
     2041 gtgaacacct gcggcttcat cgacgcggcg aagaaagagt ccgtggacgc gattctcgaa
     2101 gcggcgcgtc tgaagtcgga ggggaactgc cggcgggtgg tcgtcgcggg ctgtctggtc
     2161 gagcgttacc gcgacgagct gcgcgcggag ctgcccgagg tggacgcctt catcggcacg
     2221 aaccagatca acgacatcac gaccgtctgc gacgagaagg tgaacacgcg gcagctcccc
     2281 gtgctcccgc tcggcaacca gagcgcgacc tatctgtacg acgagtccag cccgcgcgtg
     2341 ctcgccacgc cgggccactt cgcctacgtc aagatcgcag agggctgcga ccgcccatgc
     2401 gccttctgct tcatcccgca gatgcgcggc cacttccgca gccgccgctt cggctccgtc
     2461 atcgccgagg cgcaggcgct cgccgcgcgc ggcgtcaaag agctgatcct cgtcgcgcag
     2521 gactcttcgc gctacggcga agatctcggc gagagggacg cgctcgcgca cctcatgcgc
     2581 gagctgtgcc gcctcgaagg cgtcgagtgg gtgcgcgtca tgtacaccta cccgacgcac
     2641 atcagcgacg ccttcctcga cgcgctcgcg gaggagccga aggcggtcaa gtacctcgac
     2701 atgccgctgc aacacgcctc ggcgaacgtg ctgaagctca tgaagcgcgg cggcaaccgc
     2761 gcctcgctcg aacggctcat cgaaaaggtg agaaggcgcg tgcccggcgt cgccgtccgc
     2821 acgacgt
//
LOCUS       DBNGOH010000719         4426 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1208467, whole genome shotgun sequence.
ACCESSION   DBNGOH010000719 DBNGOH010000000
VERSION     DBNGOH010000719.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4426)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4426)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4426
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1208467"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(217..1107)
                     /locus_tag="AC159P_13930"
     CDS             complement(217..1107)
                     /locus_tag="AC159P_13930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696595.1"
                     /translation="MTSKYRFLLFLVLLVGLTCGSSNVSAQAGGDKLLVAGEKQLRQS
                     DVDAVVDFYEWAIDVKFTAGQRVEFRSLTEADFRNDPVTARKDIDELLDMRAKVKAMD
                     EDRRREVRGNFIGEFIKGLRQHTADAATRLLLSIYDASRGGAGESEVAAGDEAPGSGG
                     DVSALAGKWVWARTGATSWSPNGTYAGGNGSRFTYQFSPGGAVEYAGIMNVMMGGCSQ
                     QFFLSKKGRARLSGGTLTIDWSHATSTRDFSCDRAHNYMKTLPAQTETLEVTFKTSST
                     GQRQLCTVSGGSETCFSPAE"
     gene            complement(1616..1897)
                     /locus_tag="AC159P_13935"
     CDS             complement(1616..1897)
                     /locus_tag="AC159P_13935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696596.1"
                     /translation="MLALREVRAIYHQGVYAVAAAFRQLYEMIEVEDERVYRLVAAAN
                     AAHLRKIEQLTARIHRIEEELAGIMFAVWKDGTEFDPRLLRRGDRAAVT"
     gene            complement(1923..3818)
                     /locus_tag="AC159P_13940"
     CDS             complement(1923..3818)
                     /locus_tag="AC159P_13940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4214 domain-containing protein"
                     /protein_id="HIR3696597.1"
                     /translation="MGSLPPSGHFGNQFLVTGLAAAPLPAVLHGGEGWRLVDNGLTNL
                     AVAALAIDPVRPERVYAGTIGGGVFKSTDGGSIWTNVGLNARFGTALAINPVSPNLVY
                     AGTADAGSCNARPLFTSTNGGESWGNQSSMADCDISLLVIDPTTPSTLYAGSGIQYFE
                     NDGTHLWKSTDGGATWRGSIQLTPNLGLASYGLTINPADPQILYAPGDLYGNARVIDS
                     GLFTSADGGVNWRPTGLTNTYVQAVAIDPFTHDTLYAGSLDGSRRLTPIPFRGLFKSR
                     DGGSSWFAINNGLANVTTINNIVIDRDNTNVLYVGTDGGVFKSVDGGASWSAFNNGLT
                     NSEVFSLALAPGNPNTLYAGTRVGVFKVTDHTPGPNPIDDSQFFVRQHYLDFLSREPD
                     PAGLAFWTNEITSCGTDARCVEVKRINVSAAFFLSIEFQETGYLAYRMYKAAYGDAAS
                     PGVPGTVPAIRLGEFVPDMRKVAEGVEVGIGDWQTRLEANKSAFALEFVQRGRFLGAY
                     PATMSPADLVNGLDARAGGVLSPQERDQLVAELTADDSTAGRASVLRKIAEDDGLVRN
                     EFNRAFVLMQYFGYLRRNPDDLPDANFGGWQFWLDKLNAFDGNFVEAEMVKAFISSGE
                     YRQRFGQ"
     gene            complement(3943..>4426)
                     /locus_tag="AC159P_13945"
     CDS             complement(3943..>4426)
                     /locus_tag="AC159P_13945"
                     /inference="COORDINATES: protein motif:HMM:NF019498.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR3696598.1"
                     /translation="AVGTIPHSDLFDGPATVTVRTLTIKPGEVLAWHYHPGYAFNVVK
                     SGTLTVEDGCGGPEETLTPGQAFEELAGHVHRAKNLSATEDVVVYNTFVVPQGRPTTV
                     NIPGNERRCGPPSTAEECKDGGWMNFTQPRSFADQGDCVQYVRHKPRLVLPVPEDPLP
                     "
BASE COUNT          771 a         1395 c         1404 g          856 t
ORIGIN      
        1 gtttagccgc tcgtggaacg ggctgtggaa tacgctcaag ctcaaggtgc tgcattacaa
       61 cttggttcat gccggcgtcg tgtcagcctg aatccaactc atgactcgga ttacgttaaa
      121 tgctacctaa gccaaggggc gcgtcacgct gcttgacgcg ccccttttca agtgggaatg
      181 aattgatcag gttaggtggc agccttctgt gccgtattac tccgccgggc tgaaacaggt
      241 ctcgctgccg cccgagacgg tgcacagctg ccgctgtccg gtcgaagagg ttttgaaagt
      301 gacttcgagc gtttcggtct gggcggggag cgtcttcatg tagttgtgag cccggtcgca
      361 ggagaaatcc cgcgtggagg tggcgtgcga ccagtcgatc gtcagggtcc cgccgctcag
      421 gcgggcgcgc cccttcttgg acaggaaaaa ctgctggcta cagccaccca tcatgacgtt
      481 catgatcccc gcgtactcga cggcaccgcc cggcgaaaac tggtaggtga agcgcgagcc
      541 gttgccgccg gcgtaggtgc cgttcggact ccagctggtg gcaccggtgc gggcccagac
      601 ccacttcccg gccagcgccg agacgtcccc gccggagccc ggggcctcgt cgcccgcggc
      661 cacttccgat tcacccgcgc cgccgcgcga cgcgtcgtaa atgctcaaaa gcagcctcgt
      721 cgctgcgtct gcggtgtgct gtcgcaagcc cttgatgaat tcgccgatga aattcccgcg
      781 cacctcgcgg cggcggtcct cgtccatggc tttcactttc gcgcgcatgt cgagcagctc
      841 gtcgatgtct ttgcgcgcgg tgaccgggtc gttgcgaaaa tcggcttcgg tcagcgaccg
      901 aaattcaacg cgctgcccgg cggtgaactt cacgtcgatc gcccactcgt aaaaatcgac
      961 caccgcgtcg acgtccgact gccggagctg cttttcgccc gcgaccagca gcttgtcgcc
     1021 accggcctgt gccgaaacgt tcgacgaccc gcacgtcagg ccgacgagca ggaccagaaa
     1081 gagaagaaac cgatatttgc ttgtcatttt gtgattcccc ctgaagattc gggccgagaa
     1141 ggtcgacccc gactgttaac gcggaatatc gtgcggaaag gtgtcagccc gccctaaaat
     1201 tatcgggaag gaaaggcgag tgtggggggc gcggcggcgc gggtggcgca cgcccctcca
     1261 cgctcgaggg cgagtcggtg ctccacggag tcgacccctt cacaattacg gaatcgccgc
     1321 tagaagacag atcgatgggt tcacgtccgg ctctctgtga agcggcagta agtgtcaaga
     1381 gtttttatct caggtgcccg cctgccacga gcacaccggc accgtgcaac gagttcctct
     1441 cacctctctg tccgcgctct catgcgggcg gttactttag cccggcgcat gtttcgattc
     1501 gggggcgggc ggcgctattc cttcgtcggc gctctatggg ccaagccggt tgccgcgctc
     1561 gcccgcccgt gattcgaacc cgctgaactc agtctttcga tacctatggc gacggctagg
     1621 tcaccgccgc ccggtcaccg cgccgcagca gtcgcgggtc gaactccgtc ccgtctttcc
     1681 agaccgcgaa catgatgccg gccagttcct cttcgatgcg atggatgcga gcggtgagct
     1741 gttcgatctt ccggaggtgt gccgcattcg ccgcggcgac gagacggtac acgcgctcgt
     1801 cctccacctc gatcatctca tagagctggc ggaaggccgc cgcgaccgcg tagacgcctt
     1861 ggtggtaaat ggccctgacc tcccgcagcg ccaacataat gcaacatgcc cgcgggctga
     1921 tactactgcc cgaagcgttg tcggtactcg ccggaggaga tgaacgcctt gaccatctca
     1981 gcctcgacga agttgccgtc gaaggcgtta agcttgtcga gccagaactg ccaccccccg
     2041 aagttcgcgt ccggcagatc gtccgggttc cgccgcaggt agccgaagta ctgcatcagc
     2101 acgaacgccc ggttgaactc gttacggacg aggccgtcgt cttcggcgat ttttctgagc
     2161 acgctcgcgc gcccggccgt cgagtcgtcc gccgtcaact cggcgacgag ttggtcgcgc
     2221 tcctgtggcg acagcactcc gcccgcccgc gcgtccagcc cgttaacgag atcggcgggc
     2281 gacatcgttg ccgggtaggc gcctaggaat cgcccacgct gcacgaactc aagggcgaaa
     2341 gcgctcttgt tcgcttcaag ccgggtctgc caatcgccga tcccgacctc caccccctcc
     2401 gcgaccttcc gcatgtcggg cacgaactct ccgagccgga tggcgggcac cgtgccgggc
     2461 acgcccggcg aggcggcgtc gccgtaggcc gccttgtaca tccggtaggc gaggtaacct
     2521 gtttcctgga attcgatgga gaggaagaag gccgcggaga cattgatccg cttgacctcg
     2581 acgcagcgcg cgtctgtgcc gcaggaggtg atctcgttcg tccagaaggc gaggcccgcg
     2641 gggtcgggct cgcgcgagag gaagtcgagg tagtgctggc ggacgaagaa ctgcgagtcg
     2701 tctatcgggt tgggtcccgg cgtgtggtca gtgaccttga acacgccgac gcgagtaccg
     2761 gcgtagaggg tgttcgggtt gcccggagcc agagccaggc tgaagacttc cgagttagtc
     2821 aggccgttgt tgaaggcgct ccagctcgcg ccgccgtcaa cgctcttgaa gacaccgccg
     2881 tcggtgccta cgtacaggac gtttgtattg tcccgatcta tgacgatgtt attgatcgtc
     2941 gtgacgttgg ccaggccgtt gttgattgcg aaccagctcg acccgccgtc cctgctcttg
     3001 aacaagccac gaaaaggtat cggcgtgagc ctcctactgc cgtccaaact tccggcgtac
     3061 agtgtgtcgt gggtgaaggg gtcaatcgcc acggcttgga cgtaggtgtt ggttaggccg
     3121 gtaggccgcc agttaacacc tccgtcagcg ctggtgaaga gcccactatc aatcaccctg
     3181 gcattgccgt aaaggtcacc cggtgcgtaa agaatctgcg ggtcggccgg gttgatcgtt
     3241 aacccataag aggccaggcc gaggttgggt gtcagttgga tactacctct ccatgtcgcg
     3301 ccgccgtcgg tgctcttcca gaggtgtgtg ccatcatttt cgaaatattg aatccccgac
     3361 cccgcgtaca aagtgcttgg ggtggtaggg tctatcacga gaaggctgat gtcacagtcg
     3421 gccatgctgc tctggttgcc ccagctctcc ccgccgttgg tgctcgtgaa caaaggacgg
     3481 gcattgcagg agcccgcgtc ggcggtaccg gcataaacaa ggttggggct gacggggttg
     3541 atcgccagag cagtcccgaa acgggcgttc agcccgacgt tggtccaaat tgagccgccg
     3601 tccgtgctct tgaaaacccc acccccgatt gtgcccgcgt aaactctctc cgggcggacg
     3661 gggtcaatcg ccagtgcggc gacggcaagg ttggtcagcc cgttgtcgac aagcctccag
     3721 ccctccccgc cgtgcaggac ggcggggaga ggggccgcgg ccagacctgt cacgaggaac
     3781 tgattcccga agtggcctga cggggggaga ctgcccaaca agattgacgc cgtcagagcc
     3841 agactgaaca agcagctagt agcttttctt tggctttcca taccagtccc ctcaaacatt
     3901 gggtaggcca acactcttcc gcacaccctc taatcttgcg agttagggca gcgggtcttc
     3961 ggggacgggc aggacgagcc gcggcttgtg gcggacgtac tggacgcagt cgccctggtc
     4021 ggcgaagctg cgcggctgtg taaaattcat ccagccgccg tccttgcact cctcggcggt
     4081 gcttggcggg ccgcaccgcc gctcgttccc agggatgttg acggtcgtcg gcctcccctg
     4141 cgggacgacg aaggtgttgt agacgacgac gtcctccgtg gcgctcaggt tcttcgcccg
     4201 gtggacgtgg cctgccaact cctcgaaagc ctgccccggg gtgagcgtct cctccggccc
     4261 gccgcacccg tcctcgacgg tcagcgtgcc gcttttgacg acgttgaagg cgtacccggg
     4321 gtggtaatgc caggcgagca cctcgccggg cttgatggtg agcgtgcgca ccgtcaccgt
     4381 ggccgggccg tcgaagaggt cagagtgcgg gatggtgccg acggcg
//
LOCUS       DBNGOH010000720         2876 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1025387, whole genome shotgun sequence.
ACCESSION   DBNGOH010000720 DBNGOH010000000
VERSION     DBNGOH010000720.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2876)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2876)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2876
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1025387"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..801)
                     /locus_tag="AC159P_13950"
     CDS             complement(<1..801)
                     /locus_tag="AC159P_13950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696599.1"
                     /translation="MGDLLLFLYAAAFLRQYLWVVPDNRLAWALTALAAALVFRLHWR
                     TKDVDAAGTPAVFWPLVALPLFFFYALRAALPDMSWDVLDYRLINAERALTGWPMRAG
                     DFFPVRFPFNPAPDMVMGLSRRLLGYRLGTLVNFAVLVWTGTVLEKLLRTFVTRATLR
                     CLCVLLLLLTEHLLFEVNNYMVDLLALPLMLEATRLALSTPADACARRRLFARIGLYL
                     GAGLAFKLTNLAFAIPIVLLCAYRVATSEGKQRRFDMKATLYGVVCLLL"
     gene            1072..1281
                     /locus_tag="AC159P_13955"
     CDS             1072..1281
                     /locus_tag="AC159P_13955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696600.1"
                     /translation="MLIPIASHTASHVFAGDIQQPPDGGDDTSLEDLGHAEREYVRGR
                     VRAELNREPTDEEIDEWLREHTEGY"
     gene            complement(1390..2031)
                     /locus_tag="AC159P_13960"
     CDS             complement(1390..2031)
                     /locus_tag="AC159P_13960"
                     /inference="COORDINATES: protein motif:HMM:NF016836.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HIR3696601.1"
                     /translation="MSYDEEQHKRSRVVVNTPTERREVVHQQTTRYPERSGFSGGMVA
                     AVALTAIAATAIIFLFLMNRGDEASETNINVRTSTATQPTPLAQVPVIVQTPMTQPTP
                     IVIQQAPPVIIQAPPTTTTTTTAPPTTTTTAPAPSSSGTDDTSLQLNVERAFRDDPDI
                     AVTGIIATVLEGKATLTGEVKTPALKQKAERLVRSVKGIASVENKITVNPDAP"
     gene            complement(2177..2581)
                     /locus_tag="AC159P_13965"
     CDS             complement(2177..2581)
                     /locus_tag="AC159P_13965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696602.1"
                     /translation="MRKLFAGLMTLALLLVTLLPDVYGQTRRRSRFGRKARTAAIIAG
                     CAGVGALVGGKKGAAIGAGGAGLYAFNRRAARRNFRGRTRTAGSALSGAALGAGVGGA
                     VGGKRAATVGALAGAGGGYLYRRSRPRTRRRF"
BASE COUNT          527 a          911 c         1007 g          431 t
ORIGIN      
        1 cagcagcaga cagacgactc cgtacagcgt cgccttcatg tcgaagcgcc gctgcttccc
       61 ctcgctcgtg gcgacgcggt aagcgcagag cagaacgatt gggatggcga aggcgaggtt
      121 cgtcagcttg aacgcgagtc ccgcgccgag gtagaggccg atgcgcgcga agagtcggcg
      181 ccgcgcgcac gcgtccgcgg gcgtcgagag cgcgaggcgc gtcgcttcga gcatgagcgg
      241 cagcgccagc aggtcaacca tgtagttgtt gacctcgaac agcaggtgct cggtcagcag
      301 cagcagcagg acgcacaggc agcgcaaggt cgcgcgcgtc acgaacgtgc gcaggagctt
      361 ttcgagcacc gtgcccgtcc agacgagcac cgcgaagttg acgagcgtgc cgaggcggta
      421 gccgagcagg cggcggctca ggcccatcac catgtcgggc gccgggttga aagggaaccg
      481 gacggggaag aagtcgcccg cacgcatcgg ccagcccgtc agtgcgcgct cggcgttgat
      541 caggcggtag tcgagcacgt cccaggacat gtcgggcagc gccgcccgca gcgcgtagaa
      601 gaagaagagt gggagcgcga ccagcggcca gaagacggcg ggcgtgcccg cggcgtcaac
      661 gtccttcgtg cgccagtgaa gacggaagac gagcgcggcg gcgagcgccg taagcgccca
      721 cgcgagcctg ttgtcgggga cgacccagag gtattgacgg aggaaagccg cggcgtacag
      781 gaagagcaga aggtcgccca tctccagctt ccgccgcacg ctgtcgaacg cattccgaag
      841 cactaatgaa acggccctcc gccggactct cacccgccgc ccgaaagttt atcctcgtga
      901 agaagcgcga gtttaaagcc tcgccgccga cggcgcaacg agcgtgggcg ccgacgcacc
      961 ggcgaccggc tcgccgcccc ggcgccttgc gcggcgcgcg cgcctgtgcg tactattcgc
     1021 gcaaacgaag tcttaacaac agcgacaggc gtgacgagag ggggcaggcg aatgctcatt
     1081 ccgatagctt cacacacggc gtcccacgtc ttcgcgggcg acatacagca accgcccgac
     1141 gggggcgacg acacatcgct cgaagacctg gggcacgccg agcgcgaata cgtgcgcggg
     1201 cgtgtgcgcg cggagctgaa ccgcgagccg accgacgaag agatcgacga gtggctgcgc
     1261 gaacacacgg aaggatactg aaggaagagg gggaagaggt taaaagggga aaaggcgaaa
     1321 agggagaaaa gcaagtcgct tcttcccctt ttcgcctgtt ccccttttga ccttttcccc
     1381 ccttgttcgt taaggcgcgt cggggttgac ggtgatcttg ttctcgacgc tggcgatgcc
     1441 tttgacggag cggacgaggc gctcggcctt ctgcttgagg gcgggcgttt tgacctcgcc
     1501 ggtgagcgtg gccttgcctt cgaggacggt ggcgatgatg cccgtgacgg cgatgtccgg
     1561 gtcgtcgcgg aaagccctct cgacattcag ttggagcgag gtgtcgtcgg tgccgctcga
     1621 agagggcgcg ggcgcggtgg tcgtggtcgt gggcggtgcg gtcgtcgtgg tcgtcgtggt
     1681 cggcggcgcc tggatgatga cgggcggcgc ctgttgaatg acgatgggcg tcggctgcgt
     1741 catcggcgtc tggacgatga cgggcacctg cgcgagcggc gtcggctgcg tggccgtcga
     1801 ggtgcggacg ttgatgttgg tctcggacgc ctcgtcgccg cggttcatca ggaagaggaa
     1861 gatgatggcg gtggcggcga tggccgtcag cgcgaccgcc gcgaccatgc cgccggagaa
     1921 gcccgacctc tcagggtagc gagtggtttg ctggtgcacg acctcgcggc gctccgtcgg
     1981 cgtgttgacc acgacgcggc tgcgcttgtg ttgttcttcg tcgtatgaca tgaaagcttc
     2041 gctcctgtct ctttgagttg taaaacacgc gcgaggtcgg actggaattc aaacgccttg
     2101 cggcgggcga ggcaacacgc gcgttcccgc ccgccgccgt ccacggcaac gggcgggaac
     2161 ggcgtctcgc gtcggtttag aagcgacgac gtgtgcgcgg cctcgaacgg cgatacaggt
     2221 agccgccgcc tgcgccggcg agtgcgccca cggtggcggc acgcttgccg ccgaccgcgc
     2281 cgccgacgcc cgcgccgagc gcggcgccgc tcaaggcgga gcctgcggtg cgcgtgcgcc
     2341 cgcggaagtt acgtcgcgcg gcgcggcggt tgaaggcgta caagcctgcg ccgcccgcgc
     2401 cgatggccgc gcccttcttg ccgcccacga gcgcgccgac gcccgcgcag ccggcgatga
     2461 tcgccgccgt ccgggcctta cgaccaaagc gcgaacgccg ccgcgtctgg ccgtacacgt
     2521 ccggcaagag cgtcacgagc agcagcgcca gcgtcatcaa accagcaaac agtttgcgca
     2581 taaaaaaatc tccccactac ttgaagtgcg tcccgttccc aaagcctcgc gggagagatg
     2641 acttaacaca gcggcttttc agcaaacacc attccgcacc cgccgggccg cgaatgatga
     2701 agcgaaagcg gctgaaatgc ctcgattttc gcccatccag cgcccgcccg cccgaaccat
     2761 aaacgcgccc ggcccgtcgc cgcgcgtacg aataaagtac tgaggactgt ggactgagga
     2821 ctgagtggaa ggcgtgaccc gtaacccgcg acgagtccgc acagcacgac tctcat
//
LOCUS       DBNGOH010000721         2688 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3773108, whole genome shotgun sequence.
ACCESSION   DBNGOH010000721 DBNGOH010000000
VERSION     DBNGOH010000721.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2688)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2688)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2688
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3773108"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..613
                     /locus_tag="AC159P_13970"
     CDS             <1..613
                     /locus_tag="AC159P_13970"
                     /inference="COORDINATES: protein motif:HMM:NF025006.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HIR3696603.1"
                     /translation="VVNGNLQNVFVYVKDGRTAGASPKSITGFSFAPPSEPRTLDQKG
                     CRYVPHVLGIQKDQKLQVLNSDGTSHNVNVDAAKNPKFNQGQGPNAQPIVKEFPRPET
                     LIPVKCNQHPWMRAYIGVLAHPFYSVSGQDGKFEIKGLPPGTYTVVAWHEKLGEKTQS
                     VTVGDKEQKEQNFSFAAGGTAELHVGSWTLGPTLELPGAGGHH"
     gene            727..1647
                     /gene="cyoE"
                     /locus_tag="AC159P_13975"
     CDS             727..1647
                     /gene="cyoE"
                     /locus_tag="AC159P_13975"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HIR3696604.1"
                     /translation="MKGLAIDINSEIPVVPAAGPLALRGRLAAYAELTKPRITFLIVL
                     TAAAGFALGSKGALDYVVLFHALFGIALLSSGIATLNQYMERDLDRRMRRTASRPLPS
                     GKLEPVKALVFGVALTFAAEAYLAVFVNLLTAGLGVAVVVGYLGCYTPLKTRTSLSTV
                     VGAFPGAMPPLMGWTAATGSATAEAWALFAILFMWQFPHFLAIAWMYREDYARAGICM
                     LPVVEPEGRLTAQQIVIWTLLLVPVSLFPVALGTTGAHYFYGAFAIGLLFLASSIAAA
                     FTHSRRGARRLLLASVVYLPVLFGLMVMDK"
     gene            1778..2620
                     /gene="coxB"
                     /locus_tag="AC159P_13980"
     CDS             1778..2620
                     /gene="coxB"
                     /locus_tag="AC159P_13980"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HIR3696605.1"
                     /translation="MGRALAVFIWLMTILSVWMFVNGRWWFPASIAEHGPAYDQQFML
                     TLIVVGVAFAAAQIGLGYTIWRYRDTGSGERATYSHGNNRLEVIWTSITAVIFITVAV
                     LGQRVWAQLHFQPAPADAARVLVTAQQFQWNFHYPGPDNTFGRTDPKLISDESINYTG
                     LDQQDAAAADDTVTQTLVVQLDRAVELILTARDVTHSFWVPPLRFKQDAVPGVRIPVH
                     FKPVKPGRYEIACAELCGQQHYKMKAYMLVLPEAEYREVVALKQAQFEQRLAELFKKY
                     PETY"
BASE COUNT          462 a          965 c          806 g          455 t
ORIGIN      
        1 cgtcgtcaac ggcaacctgc aaaatgtctt cgtctacgtc aaggacggca gaaccgccgg
       61 ggccagcccc aagagcatca ccggcttttc cttcgcgccg ccatcggagc cgcgcacgct
      121 cgaccagaag ggctgccgct acgtcccgca cgtcctcggc atccagaagg atcagaagct
      181 acaggtcttg aacagcgacg gcacttcgca caacgtcaac gtggacgccg ccaagaaccc
      241 gaagttcaac caggggcagg ggccgaacgc gcagcccatc gtcaaagagt tcccgcgccc
      301 cgagacgctg atcccggtca agtgcaacca gcacccgtgg atgcgcgcct acatcggcgt
      361 cctcgcgcac ccgttctatt cggtcagcgg gcaggacggc aagttcgaga tcaagggcct
      421 gccgcccggc acttacaccg tcgtcgcgtg gcacgagaag ctcggcgaga agacgcagtc
      481 cgtcaccgtc ggcgacaagg agcagaagga gcagaacttc agcttcgcgg cgggcggcac
      541 cgccgaactc cacgtcggct cgtggacgct cggccccacg ctcgaactcc ccggcgccgg
      601 cggccaccac tgaaagagag aagtcaggag tcggaagtcg gaagtcggaa tgaaaagacc
      661 gcaaccgctt ctgttttatt ctgacttccg acttccgact cctgacttcc tatcaaggtt
      721 tacttcatga agggtctcgc aatcgacatc aactccgaaa tcccggtcgt gcccgcggcg
      781 gggccgctcg cgctgcgcgg gcggctcgcc gcctacgccg agctgaccaa gccccgcatc
      841 acgttcctga tcgtgctcac cgcagccgct ggcttcgccc tcggctcgaa gggcgcgctc
      901 gactacgtcg tgctgttcca cgccctcttc ggcatcgcgc tcctctcctc ggggatcgcg
      961 acgctcaacc agtacatgga gcgcgacctc gaccgccgca tgcgccgcac ggcgtcgcgc
     1021 ccgctcccct cggggaagct cgaaccagtt aaagcgctcg tcttcggcgt cgcgctgacg
     1081 ttcgcggcgg aagcctacct ggcagtcttc gtcaaccttc tgacggcggg cctcggcgtc
     1141 gcggtcgtcg tcggctacct cggctgctac acgcctctga agacgcgcac ctcgctctcg
     1201 acggtcgtcg gcgccttccc cggcgcgatg ccgccgctca tgggctggac cgccgccacc
     1261 ggcagtgcca ccgccgaggc gtgggcgctc ttcgccatcc tcttcatgtg gcagttcccg
     1321 cacttcctcg ccatcgcgtg gatgtaccgc gaggactacg cgcgcgcggg catctgtatg
     1381 ctgcccgtgg tcgagccgga gggccgcctg accgcgcagc agatcgtcat ctggacgctg
     1441 ctgctcgtgc ccgtctcgct cttccccgtc gctttgggca cgaccggcgc gcactacttc
     1501 tacggggcct tcgccatcgg cctgctgttc ctcgcgagca gcatcgccgc ggccttcacg
     1561 cattcgcgcc ggggcgcgcg caggctgctg ctcgcctcgg tcgtctactt gcccgtcctc
     1621 ttcggtctga tggtcatgga caaatgattg cggaatgcgg agttcggatt gcggagtggt
     1681 cgactgtcga aagtggaacc tccgaaatcc ggcttcaagc agaagcagca cgacaatccg
     1741 caatccgaaa tccgcattcc gcaattagga gaatcctatg ggccgtgcgc tggctgtctt
     1801 catctggttg atgacgatcc tctccgtctg gatgttcgtg aacggcaggt ggtggttccc
     1861 ggcctccatc gccgagcacg ggccggccta cgaccagcag ttcatgctga cgctcatcgt
     1921 cgtcggcgtg gccttcgccg ccgcgcagat cggcctcggc tacacgatct ggcgctaccg
     1981 cgacaccggc agcggcgagc gcgccaccta ctcgcacggc aacaaccggc tggaagtcat
     2041 ctggacttcg atcacggcgg tcatcttcat cacggtcgcc gtgctcggcc agcgcgtctg
     2101 ggcgcagctc cacttccagc ccgcccccgc ggacgccgcc cgcgtcctcg tcaccgccca
     2161 gcaattccag tggaacttcc attaccccgg ccccgacaac accttcgggc gcaccgaccc
     2221 gaagctcatc agcgacgagt cgatcaacta cacgggcctc gaccagcagg acgccgccgc
     2281 cgcggacgac accgtcacgc agacgctcgt cgtccagctc gaccgcgcgg tcgagctgat
     2341 cttgacggcc cgcgacgtga cgcacagctt ctgggtgccg cctctcaggt tcaagcagga
     2401 cgccgtgccc ggcgtcagga ttcccgtcca cttcaagccg gtcaagccgg gccgctacga
     2461 gatcgcctgc gccgagctgt gcggccagca gcactacaag atgaaggcgt acatgctcgt
     2521 gctgcccgag gccgagtacc gcgaggtggt ggcgctcaag caggcgcagt tcgagcagcg
     2581 cctcgccgag ctgttcaaga agtacccgga aacttactga gcgttagtta aacccccgtt
     2641 cgcgcgcggc ccccgctgag aagcgccgcg gcgagaagag gagcgaag
//
LOCUS       DBNGOH010000722         1822 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3957889, whole genome shotgun sequence.
ACCESSION   DBNGOH010000722 DBNGOH010000000
VERSION     DBNGOH010000722.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1822)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1822)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1822
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3957889"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..274)
                     /locus_tag="AC159P_13985"
     CDS             complement(<1..274)
                     /locus_tag="AC159P_13985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696606.1"
                     /translation="MSANTTSTNPFVQAIVEGRAPTHARLAAARGLLPLAQDDLVEVL
                     VSLRSDEDADIAAAAEATLGEQEAGVLAQLASSPASSPAVLSYLASR"
     gene            complement(397..1293)
                     /locus_tag="AC159P_13990"
     CDS             complement(397..1293)
                     /locus_tag="AC159P_13990"
                     /inference="COORDINATES: protein motif:HMM:NF015506.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HIR3696607.1"
                     /translation="MNTPRTGGWRRTVVALSLTLCAASPASAQQPQAGAPEREQQFLV
                     EAPSPEVQRGRQLLEQGDAEGAIKVLRPATERDKPDGEAWHLLGLAYARQGKRQDALQ
                     SFERAVAVRLDRLAFGSVPNAERFVRLTAEQRAATKERALERHREALESVEAYLQQNP
                     NGADFWRAQAETIKAHAEHIAASDTEFGDVRPDSKGTRLVVNDKPDPGYTEEARRNGT
                     GGRVRLRAIFGADGKVTHPLAIRSLPHGLTEKAFEAARRISFQPATVDGRPVSQFIIL
                     EYGFEIHHRGIRPPTAPRPRFP"
     gene            complement(1438..>1822)
                     /locus_tag="AC159P_13995"
     CDS             complement(1438..>1822)
                     /locus_tag="AC159P_13995"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HIR3696608.1"
                     /translation="LGPRDADATFVRGQIEALRFYSQPVHARTGGNVFSQTDITTKAV
                     ILSKPDPGLTQEARANNAVGFVRLRAVLDADGTVKHILVVKPLPDGLMERAVQAARSI
                     KFRPATSNGVPVSQYITLEYHFSIY"
BASE COUNT          244 a          580 c          696 g          302 t
ORIGIN      
        1 ggcggctggc gaggtaggag agcacggcgg gcgaggaggc gggcgaggag gcgagctggg
       61 cgagcacgcc cgcctcctgc tcgccgagcg tcgcctcggc ggcggcggcg atgtccgcgt
      121 cctcgtcaga ccgcagagag acgagcactt cgacgaggtc gtcctgcgcg agcggcagca
      181 gcccgcgcgc cgccgcgagc cgcgcgtgcg tcggcgcgcg gccttcgacg atggcctgca
      241 cgaacgggtt ggtggaggtt gtgttggcgc tcatccaaac gtgtccgagg ttgaccgagt
      301 ccgaggttga tcgagtgcga agtcattcgc gcgcgtcggc gaaggggcgc ttgaaatcga
      361 gcgcggcttc gtcacttgaa attcaggggc gcggcttcaa ggaaagcgcg ggcgcggcgc
      421 ggtggggggg cggatgccgc ggtggtgtat ctcgaagccg tactcaagaa tgatgaactg
      481 cgagacgggc cgaccgtcca cggtcgcggg ctgaaagctg atgcggcgag cggcttcgaa
      541 cgccttctcg gtcagcccgt gggggagcga cctgatggcg agcgggtgtg tgaccttgcc
      601 gtcggcgccg aagatggcgc ggaggcggac gcgcccgccg gtgccgttgc gccgcgcctc
      661 ttccgtgtag ccggggtcgg gcttgtcgtt gacgacgagg cgggtgccct tgctgtcggg
      721 ccgcacgtcg ccgaactcgg tgtcgctggc ggcgatgtgc tcggcgtgcg ccttgatggt
      781 ctcggcctgt gcgcgccaga agtccgcgcc gttcgggttc tgttgcaggt atgcctcgac
      841 gctctcaagc gcctcgcggt ggcgttcgag cgcgcgctct ttcgtggccg cgcgctgctc
      901 cgcggtcagc ctcacgaacc gctccgcgtt ggggacactc ccgaacgcga gcctgtcgag
      961 ccgcacggcg acggctcgct cgaaagactg aagcgcgtcc tgccgcttgc cctgccgcgc
     1021 gtaggccagc ccgagcaggt gccacgcctc gccgtcgggc ttgtcgcgct cggtggccgg
     1081 gcgtagcacc ttgattgcgc cctccgcgtc cccctgttcg agcaactgcc tgccgcgctg
     1141 cacctccggt gaaggcgctt caactaaaaa ctgctgctcg cgttcgggcg cgccggcctg
     1201 cggttgctgc gcggaggcgg gcgacgcggc gcagagcgta agcgacagcg cgacgactgt
     1261 gcgccgccat ccgccggtgc gaggtgtgtt catagaatct taatcacgtt gcgacctccg
     1321 aaggccgcga aggttcggcg cgggggcgcc gcgcgcgggg gacgcggaaa ttatattcca
     1381 gcgtgacggt ctcgtgcgaa cgcgcggacg ccctccggcg gcggagaacg tcggtcgtca
     1441 gtagatgctg aagtggtatt ccagtgtgat gtactgggac acgggcacgc cgttgctggt
     1501 cgcgggcctg aacttgatgg agcgcgcggc ctgcaccgcg cgctccatca ggccgtcggg
     1561 gagcggcttc accacgagga tgtgtttgac ggtgccgtcg gcgtcgagca cggcgcgcag
     1621 gcggacgaag ccgacggcgt tgttcgcgcg cgcctcctgc gtgaggccgg ggtcgggctt
     1681 cgagaggatg acggccttcg tcgtgatgtc cgtctggctg aacacgttcc cgcccgtccg
     1741 cgcgtgcacc ggctgcgagt agaagcggag cgcctcgatc tgtccccgca cgaacgtcgc
     1801 gtcggcgtcg cgcggcccga gg
//
LOCUS       DBNGOH010000723         4102 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1025537, whole genome shotgun sequence.
ACCESSION   DBNGOH010000723 DBNGOH010000000
VERSION     DBNGOH010000723.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4102)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4102)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4102
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1025537"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(66..770)
                     /locus_tag="AC159P_14000"
     CDS             complement(66..770)
                     /locus_tag="AC159P_14000"
                     /inference="COORDINATES: protein motif:HMM:NF015510.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LolA family protein"
                     /protein_id="HIR3696609.1"
                     /translation="MKKLTLLALAFALLIGAAFVPPGAASAAAAPQGAGLVSSIFNKM
                     ERNRRDLKSLRANVTIQKVDARFGAEMSEGSVQYVPGAGREANVRLDFSKPRQEVLAV
                     ENGQYTYYKPKMNMAYKGRSNKAPKAGSLLSFVFGASSAQLKNDFNHEVVGEGTLYEG
                     GPHVVMIKLTPKGGADFKFAEIWVDGNGMPVQTRVTERNGDSTLVRLTNIQKNARING
                     DAFKISLPSGVKIVNS"
     gene            complement(839..1186)
                     /locus_tag="AC159P_14005"
     CDS             complement(839..1186)
                     /locus_tag="AC159P_14005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696610.1"
                     /translation="MSRFWRALKRIILWSYGRTTWQYDVLCVLILAFIFLTPKTWFRE
                     GELARPLPHQSSDKAARKLLIGPGVLGQSPDAQAVERGVQQLTGRTDFRVRGWRALRA
                     EDGQIAAYEVDIE"
     gene            complement(1268..1975)
                     /locus_tag="AC159P_14010"
     CDS             complement(1268..1975)
                     /locus_tag="AC159P_14010"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3696611.1"
                     /translation="MRKILQAENLVKTYRVGSVDVQALRGVSLEVEEGEFVAIMGPSG
                     CGKSTMLHLLGGLLTPTTGRIVIDDEDLTAASDARRTDIRRRKIGFVFQRFNLFPTLT
                     AEGNLRLAERIHAGGDGGKKEDQPERRREILRLLKLEEKMHHKPLELSGGEQQRVALA
                     RAVVNRPALVLADEPTGNLDTENSAIVLNMFQEMNYRFNQTIVMITHNPEAAAMCRRI
                     IQMRDGQIVASAPRVQI"
     gene            complement(2046..3140)
                     /locus_tag="AC159P_14015"
     CDS             complement(2046..3140)
                     /locus_tag="AC159P_14015"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3696612.1"
                     /translation="MDNLIAANVRQRPVRALVSAAGVALGVCLVMLFTGLARGLSNDM
                     KRRSQNLRAEILFTKGGGDMLSAAANVDVRYAGELLKVEGVAMAVPAIRYVSQANRGL
                     GLEQIEGVDWQPFAEMNGTRLVEGREPADLREVVVDETKARNNDLRVGSEIKLFGDLP
                     YTVVGIYTPEAGPRVKMSLAAMQDAMEAPGKATWVYVKVKNPGEEERVARRIDEAEPG
                     NKIQFTRDVFRGVEASIPLLPVFLRALVGLAAVVSALVVMLAMYTTITERTREIGVLK
                     ALGASRGYIVGVIEKEAVLISVLGVAAGFAISFAAAFLIQRFTGLVFEYSWTWALTAL
                     LIGIAGGVLGALYPAVRAANLDPVSALSYE"
     gene            complement(3259..>4102)
                     /locus_tag="AC159P_14020"
     CDS             complement(3259..>4102)
                     /locus_tag="AC159P_14020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696613.1"
                     /translation="LDTSFAQCGIADLYRTAEGRLIVPRPGQIYLDISFLTYKIAEMS
                     SNGERFWAALKQGDEKYRNFVTGRNSAAYGKLSANGDAANPDCRRGGKVEQAARERAT
                     VSALSSLRPQHFSDALLVPPSVTDESVYYARGEDFQEEPDTRVGAKKSARVVRSYYVL
                     SEFTTAGPGRATLLRRFWFDRVGAIRLARVQTYDERGQLITDVVYGAQKPFSQGQLLP
                     AQLELTRPQEHYSIRIAFQDPTEVRVNQPFGPETFVLKNESNLREVDLDAMKKEGGAV
                     NRQP"
BASE COUNT          662 a         1341 c         1348 g          751 t
ORIGIN      
        1 gaagggcttc ggaaccttgg ggagcgccga aaccctttgc cgctgtcgtg aaattgtcga
       61 gagctttagc tgttgacgat cttcacgccc gaggggagcg aaatcttgaa ggcatcgccg
      121 ttgatgcgcg cgttcttctg gatattggtc aggcgcacga gcgtcgagtc gccgttgcgc
      181 tcggtgacgc gcgtctggac gggcatgccg ttgccgtcca cccagatttc ggcgaacttg
      241 aagtcggcgc cgcccttcgg cgtcagcttg atcatcacga cgtgggggcc gccctcgtag
      301 agcgtgcctt cgccgaccac ctcgtggttg aagtcgttct tcaactgcgc gctggaggcg
      361 ccgaaaacga agctgagcag gctgcccgcc ttgggcgcct tgttcgagcg gcccttgtag
      421 gccatgttca tcttcggctt gtagtaggtg tactgcccgt tctcgacggc gagcacctcc
      481 tgccgcggct tgctgaagtc gaggcggacg ttggcttcgc gcccggcgcc cggcacgtac
      541 tgcacgctgc cctcggacat ctccgcgccg aaccgcgcgt ccaccttctg gatggtgacg
      601 ttggcgcgca ggctcttgag gtcgcggcgg ttccgctcca tcttgttaaa gatcgagctg
      661 acgaggcccg cgccctgcgg cgcggcggcg gctgaggccg cgcccggcgg gacgaacgcg
      721 gctccgatca agagtgcgaa cgcgagggca agcaaagtta acttcttcat gcaaactcct
      781 tcgaaagtat cagggagact cctgcccggc cccgaatccg ggtgattata gggaataatt
      841 actctatgtc aacctcgtag gcggcgattt ggccgtcttc ggcccggagc gcccgccacc
      901 ccctgacgcg aaaatcagtc cgccccgtga gttgttgcac gccacgttcc acggcctgtg
      961 cgtcggggga ttgccccaga actccggggc cgatgagcag cttgcgggcc gctttgtccg
     1021 agctttgatg cggaagtggg cgggccagtt cgccctcgcg gaaccacgtt ttcggcgtca
     1081 ggaagatgaa ggcgaggatg aggacgcaca gcacgtcgta ctgccaggtg gtgcgaccgt
     1141 aagaccagag aatgatgcgc tttagggcgc gccagaatcg agacatgcca caaaaagatg
     1201 tcagatgtgc ggggccgggt gctggaaaaa aatggcagcc ggggcgcccg gcccgcggac
     1261 gcctgaatta tatctgtacg cggggggcgg acgcaacgat ctgcccgtcg cgcatctgta
     1321 tgatgcggcg gcacatggcg gcggcctcgg ggttgtgggt gatcatcacg atggtctggt
     1381 tgaagcggta gttcatctcc tggaacatgt tgaggacgat ggccgagttc tccgtgtcga
     1441 ggttgccggt cggctcgtcg gcgaggacga gggccgggcg gttgacgacg gcgcgggcca
     1501 gcgcgacgcg ctgctgctcg ccgcccgaca gctccagcgg cttgtggtgc atcttctctt
     1561 cgagcttgag caggcggagg atttcgcggc gccgctcggg ctggtcttcc tttttgccgc
     1621 cgtcgccgcc cgcgtggatg cgctcggcga gccgcaggtt cccttccgcc gtgagcgtcg
     1681 ggaagaggtt gaagcgctgg aagacgaagc cgatcttgcg ccgccggatg tccgtgcgcc
     1741 gcgcgtccga ggcggcggtg aggtcttcgt cgtcgatgac gatgcgcccg gtcgtcggcg
     1801 tcagcaagcc ccccaagagg tgcagcatcg tcgatttgcc gcaccccgaa ggccccatga
     1861 tcgccacgaa ctcgccctcc tcgacctcca aagacacgcc gcgcagcgcc tgcacgtcca
     1921 cgctgccgac gcggtaggtc ttgacgaggt tctctgcctg aagaattttt ctcatttgta
     1981 aatggtgaat ggtaaacggt aaatggggag aagacaaaaa ctattcacca ttcaccattc
     2041 accattcact cgtaagatag cgcgctcacc gggtccagat tggcggcgcg caccgccggg
     2101 tagagcgcgc cgaggacgcc gcccgcgatg ccgatgagga gcgccgtcag cgcccacgtc
     2161 caactgtact cgaagacgag gcccgtgaag cgctggatca ggaacgcggc ggcgaacgag
     2221 atggcgaagc ccgccgcgac gccgaggacg ctgatgagca cggcctcctt ctcgatgacg
     2281 ccgacgatgt agccgcgcga ggcgccgagc gccttcagca ccccgatctc gcgcgtccgc
     2341 tccgtgatgg tcgtgtacat cgccagcatc acgacgagcg cgctcaccac cgccgccagt
     2401 cccaccagcg cgcgcaggaa gaccggaagt agcgggatgc tcgcttcgac gcccctgaac
     2461 acgtcgcgcg tgaactggat tttgttgccc ggctccgcct cgtcgatgcg ccgcgcgacc
     2521 cgttcctcct cgccggggtt cttcaccttg acgtagaccc acgtggcctt gcccggcgcc
     2581 tccatcgcgt cctgcatcgc ggccagcgac atcttcacgc gcggcccggc ctccggcgtg
     2641 tagatgccga ccaccgtgta cggcagatcg ccgaagagct tgatctcgct cccgacgcgg
     2701 aggtcgttgt tgcgcgcctt cgtctcgtca acgacgactt ctctgaggtc tgccggctcg
     2761 cgcccctcga cgaggcgcgt cccgttcatc tcggcgaacg gctgccagtc cacgccctcg
     2821 atctgctcca gcccgaggcc gcggttggcc tgcgagacgt agcggatggc gggcacggcc
     2881 atcgcgacgc cctcgacctt gagcagctcc ccggcgtacc gcacgtccac gttcgccgcc
     2941 gcgctcagca tgtcgccgcc acccttcgtg aacagtatct ccgcgcgcag gttctgcgag
     3001 cgccgcttca tgtcgttcga cagcccgcgc gccagccccg tgaacagcat caccagacac
     3061 acgcccagcg ccacgcccgc cgcgctcacg agcgcgcgca ccggtcgttg tcggacgttg
     3121 gcggcgatca ggttatccat taaaaccgtg aactgtgaac cgtgaaccgt taaagtccgt
     3181 gaaccgtcaa ctgtgatgag tcggaaccgt ctttcactga tcacggttta cggttcacaa
     3241 ttcacggact ttaacggttc acggctgacg gttcacggcc cctccctctt tcttcatcgc
     3301 gtccagatcg acttcgcgca ggttcgactc gttcttgagg acgaaggttt cggggccgaa
     3361 gggctgattg accctgacct cggtcgggtc ctggaaggcg atgcggatcg agtagtgctc
     3421 ctgcgggcgc gtcagttcca actgcgcggg caggagctgc ccctggctga agggcttctg
     3481 cgcgccgtag accacgtcgg tgatgagctg cccgcgctca tcgtaggtct gcacgcgcgc
     3541 gaggcggatg gcgccgacgc ggtcgaacca gaagcggcgg agcagcgtcg cgcggcccgg
     3601 ccccgcggtc gtgaactcgg agagcacgta gtagctgcgc acgacgcgcg cgctcttctt
     3661 cgcgccgacg cgcgtgtccg gctcctcctg aaagtcctcg ccgcgcgcgt agtagaccga
     3721 ctcgtccgtc acggagggcg gcacgagcag cgcgtcgctg aagtgctgcg ggcgcagcga
     3781 ggagagcgcg ctcacggtgg cgcgctcacg ggccgcctgc tccaccttgc cgccccggcg
     3841 gcagtccggg ttggcggcgt cgccgttcgc cgagagcttg ccgtaggcgg cgctgttcct
     3901 cccggtcacg aagttgcggt acttctcgtc gccctgcttg agcgcggccc agaagcgctc
     3961 gccgttcgaa ctcatctcgg cgatcttgta ggtgaggaag ctgatgtcga ggtaaatctg
     4021 gccggggcgc ggcacgatga ggcgcccctc cgccgtgcgg tagaggtcgg cgatgccgca
     4081 ctgcgcgaag ctcgtgtcga gg
//
LOCUS       DBNGOH010000724         3258 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2856993, whole genome shotgun sequence.
ACCESSION   DBNGOH010000724 DBNGOH010000000
VERSION     DBNGOH010000724.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3258)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3258)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3258
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2856993"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            223..747
                     /locus_tag="AC159P_14025"
     CDS             223..747
                     /locus_tag="AC159P_14025"
                     /inference="COORDINATES: protein motif:HMM:NF016836.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HIR3696614.1"
                     /translation="MKKTRIAALVAGTALAALSVGCGDAANTNTVNANRVNSNTAVVV
                     NSNNANVANVNTSAGAYNRNYNARGDYDKDANTYKSEAKNKGESIGQGLEDGWLHFKT
                     RAALAAVDDLRDSTINVDVDNAVITLRGDVGSAAQKAAAEKAAKGIEGQKGVKNNLAV
                     KPAGSGTNTNAKGK"
     gene            1107..3152
                     /locus_tag="AC159P_14030"
     CDS             1107..3152
                     /locus_tag="AC159P_14030"
                     /inference="COORDINATES: protein motif:HMM:NF014081.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR3696615.1"
                     /translation="MRKSVNRFCAAFVAASVVAVVCARTVAPPVAAQRSTGTDAYAIT
                     GARIVTGTGATVERGTVVFRDGLIVAVGERAAAPPDARTIDGAGLTVYPGLIDASTNL
                     GIPRPTPTPATGPPSPFAGLFGPPVSPSAVSPNSTQLPGLQPEIAAADIVRPGGAEIE
                     AARSAGITAAQTAPRGNIFLGQSAIINLAGDTPQQMVVRSPVALYVGLTPLSGGQYPG
                     SLMGVFSSVRQLLLDAQRYREAQQIYERNPKGLRRPGQDKSLEALLPVLARQMPVVMQ
                     ADREREIDRALDLAQEFNLRMIIVGGTESHRLAARLKSMDVPVLLSLNFPRRTAAASP
                     DSDPESLRVMRDRVEAPKTAGRLAAAGVRFAFQSGGMTTISDFLANAARAVENGLGRD
                     DAVRALTIRPAEILGVSAQLGSIEVGKIANLTVARGDLLAKERRITHVFIDGRPVDLR
                     PPTGPGAGASGATGTWSVTVNLGGGIAEQTVTLNLQQQGERLTGSLQGALGSGQIGAG
                     NVTGSDIRFNVPVTLPAPASQTTDAQFTGTITGNTMSGTVQPVGRDAGTFTATRQGGA
                     APAGTPPAGGQPTPPAEASLAGTWSLEFDAGTRVIPATLTLRQEGARLTGTLSSELGT
                     TEITDGSVSGQSFRFSATVTIQQTFSITVEGTVTGNRMSGTAQSPNGPAPFTGTRNP"
BASE COUNT          564 a         1248 c          995 g          451 t
ORIGIN      
        1 ttcttcactg accactgacc accggccact gaccactttc ccccttgtcc tcacgcccgc
       61 cgcggcgcta atataagcgc cagttcaagc ccagacgctt cagcgagttt ttcaagtgag
      121 acgggggcgc gtgcgcgcaa cgcgagcggc gctcccgcgg ttcgactacg cgcgggggct
      181 tgcatcttgt ccggaggaca cttaaacatc acaggagatt ttatgaagaa gaccaggatc
      241 gcagctctcg tggcgggcac ggccctcgcc gcgctctcgg tcggttgcgg cgacgcggcc
      301 aacaccaaca ccgtgaacgc caaccgcgtc aactcgaaca ccgccgttgt cgtcaactcg
      361 aacaacgcga acgtcgccaa cgtcaacacc agcgcgggcg cctacaaccg caactacaac
      421 gcgcgcggcg actacgacaa ggacgcgaac acctacaagt ccgaggccaa gaacaagggc
      481 gagtccatcg gccagggtct cgaagacggt tggctccact tcaagacgcg cgccgcgctc
      541 gccgccgtgg acgacctgcg cgactcgacc atcaacgtgg acgtggacaa cgccgtcatc
      601 acgctgcgcg gcgacgtggg cagcgccgcc cagaaggccg ccgccgagaa ggccgcgaag
      661 ggcatcgagg ggcagaaggg cgtcaagaac aacctcgccg tcaagcccgc cggcagcggc
      721 accaacacca acgcgaaggg taaatagtaa gaaggggtga caaagagaag gggagacggg
      781 gtgaaagaga gacgtttgac aacgcagctt ctttcacccc gtcccccttt ctctttgtcg
      841 ccccgtctca gccgcagcgc ggctgaaact ttccccgccg ctgtcgcgta agcagcggcg
      901 cgctcggaca caggcattcc caccgttcgt tttattggat tgcgtctagg gtttcggatg
      961 cgggccgcgc gggtcgcccg cggcgtgcgc cttcgttccg gaacgtccaa acaaaattta
     1021 agaacgcgac ctcccggcgc cgactcccgc cggggccgcg cgcccgtaac gacttgtcga
     1081 ttccccgctc aggaagacca acgcccatgc gaaaaagtgt gaacagattc tgcgccgcct
     1141 tcgtcgcggc gtcggtcgtg gccgtcgtct gcgcgcgcac ggtcgcgccc ccggtcgcgg
     1201 cgcagcgcag caccggcacg gacgcctacg cgatcacggg cgcgcgcatc gtcaccggca
     1261 ccggcgccac ggtcgagcgc ggcacggtcg tcttccgcga cgggctgatc gtcgccgtcg
     1321 gcgagcgcgc cgccgccccg cccgacgcgc gcaccatcga cggcgcgggt ctcaccgtct
     1381 atcccggcct catcgacgcc agcaccaacc tcggcatccc gcgccccacg ccgacgcccg
     1441 ccaccggccc tccgagtccc ttcgccgggc tgttcggccc gcccgtcagc ccttcagccg
     1501 tctcgcccaa ctcgacgcaa ctgcccggcc tccagcccga gatcgccgcc gccgacatcg
     1561 tccgccccgg cggcgccgag atcgaggccg cgcgcagcgc cggcatcacc gccgcgcaga
     1621 ccgcgccgcg cggcaacatc ttcctcggcc agtcggccat catcaacctc gcgggcgaca
     1681 cgccgcagca gatggtcgtg cgctcgcccg tcgcgctcta cgtcggcctg acgccgctct
     1741 ccggcggcca gtaccccggc tccttgatgg gcgtcttctc ttccgtcagg caactgctct
     1801 tggacgcgca gcgctaccgc gaggcgcagc agatttacga gcgcaacccg aaaggtttaa
     1861 gacgacccgg acaggacaag tcgctggagg ctctgttgcc cgtgctcgcg cggcagatgc
     1921 cggtcgtgat gcaggccgac cgcgagcgcg agatcgaccg cgccctcgac ctcgcgcagg
     1981 agttcaacct ccggatgatc atcgtcggcg gcaccgagtc gcacaggctc gccgcgcgcc
     2041 tgaagtcgat ggacgtgccc gtgctcctct ctctgaactt cccgcgccgc accgccgccg
     2101 cctcgcccga ctccgacccc gaatctttgc gcgtcatgcg cgaccgcgtc gaggcgccga
     2161 agaccgcggg ccgtctggcc gcggcgggcg tccgcttcgc cttccagtcc ggcggcatga
     2221 cgaccatctc ggactttctc gcgaacgccg cgcgcgccgt cgagaacggt ctcgggcgcg
     2281 acgacgccgt gcgcgccttg acgattcggc ccgcagagat tctcggcgtc tccgcgcagc
     2341 tcggctccat cgaggtcggc aagatcgcca acctcacggt cgcgcgcggc gacctgctgg
     2401 ccaaagagcg ccgcatcacg cacgtcttca tcgacggtcg ccccgtcgat ctacgtccgc
     2461 cgaccggccc cggcgcgggc gcaagcggcg cgaccggcac gtggtctgtc accgtcaacc
     2521 tcggcggcgg catcgccgag cagaccgtca cgctcaacct gcaacagcag ggcgagaggc
     2581 tcaccggctc gctgcaaggc gcgctcggct caggccagat cggcgcgggc aacgtgacgg
     2641 gcagcgacat ccgcttcaac gtccccgtca cgctccccgc gcccgcctcg cagaccaccg
     2701 acgcgcagtt cacgggcacc atcaccggca acacgatgag cggcaccgtg cagcccgtcg
     2761 ggcgcgacgc gggcacgttc accgccacgc gtcagggcgg cgccgcgccc gcgggcacgc
     2821 cgcccgccgg aggccaaccg acgccgcccg cggaagcaag cctcgccggc acgtggtcgc
     2881 tcgaattcga cgccggcacg cgcgtcatcc cggcgacgct cacgctgcgg caggagggcg
     2941 cgcggctgac cggcacgctg tcgagcgaac tcggcaccac cgagatcacc gacggcagcg
     3001 tcagcggcca gagcttccgc ttctcggcca ccgtcaccat ccagcagacc ttcagcatca
     3061 cggtcgaagg caccgtcacg ggcaaccgca tgtccggcac ggcccaatcg cccaacggcc
     3121 ccgcgccctt cacggggacg cgcaatccgt aaaagccgtg aaccgtgaac cgtgaaccgt
     3181 gacgagttaa aaccatcaca tccgtcacgc ttcacggttc acccttaacg gctcactgct
     3241 tttcacctgt cacggttc
//
LOCUS       DBNGOH010000725         5582 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2857673, whole genome shotgun sequence.
ACCESSION   DBNGOH010000725 DBNGOH010000000
VERSION     DBNGOH010000725.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5582)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5582)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5582
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2857673"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..153)
                     /locus_tag="AC159P_14035"
     CDS             complement(<1..153)
                     /locus_tag="AC159P_14035"
                     /inference="COORDINATES: protein motif:HMM:NF013468.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
                     /protein_id="HIR3696616.1"
                     /translation="MVIPDGQEARARAAGVVGAGGVVGFRTDTFYGLGVDPFNRDALR
                     ALNELKG"
     gene            211..684
                     /locus_tag="AC159P_14040"
     CDS             211..684
                     /locus_tag="AC159P_14040"
                     /inference="COORDINATES: protein motif:HMM:NF033537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lasso peptide biosynthesis B2 protein"
                     /protein_id="HIR3696617.1"
                     /translation="MRGRLHSLSGRLAQAARFVAREPLSGLLIARMAAWVAATSLLLN
                     LLPFEKAMVIITPLRRRESVRDPAETAETQARLSRLLDLLLATDFWLYTPTCWKRAPV
                     LYRFLALSGIRTRVVFGVRRKESGALDGHAWLEADGEPLLERTAPDYTVTFSYPT"
     gene            complement(805..2085)
                     /locus_tag="AC159P_14045"
     CDS             complement(805..2085)
                     /locus_tag="AC159P_14045"
                     /inference="COORDINATES: protein motif:HMM:NF016056.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DCC1-like thiol-disulfide oxidoreductase family
                     protein"
                     /protein_id="HIR3696618.1"
                     /translation="MAPGTLARLVPATAGALGMLRAVVCGFFLLSILLTDFAALSRLP
                     ATVMRPTGAMQIFSWQFYERLLTPQGMPALKALLVASLLFGAVGYLTPLTTKCAAALV
                     LFYEGLLRSFGHFNHDEMLAVYVLVVLAFTPSGDAFSVDSLPDSNTERRGEVKYGYPV
                     LLMRALVAWAYFSSALVKLRVAGLEYFGPDSLPALAVSHSLDNLHDARFAYAFRLAEY
                     RGPLTTAAVALVLAWELLFPLAIFLRRARRLILGFGLVFHVATLFLMNFFFPFHLAMY
                     AVFVDWPAVARRVSQAPLIKRLARWWGEFRGVPESFPCVRVEECVARETLLWDGDCGF
                     CARWVERLRHFARRPFEAQPYQSIETELPTEVARWTKAQMHWVDGIGRVYGGSRALAR
                     VLAASGHTFLAALLESKPLRPFAWLGYRIAARNR"
     gene            complement(2067..2606)
                     /locus_tag="AC159P_14050"
     CDS             complement(2067..2606)
                     /locus_tag="AC159P_14050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696619.1"
                     /translation="MEDGSCRDEGRRYLPRYALLTVFLFAPLLWSFAALRNLYPFAAW
                     TVMTSPSEVARGRTYYVLRGETQAGTDVDLRAVELTDALGGRVWGMFGATAENGGFRI
                     RSPHPSNAAMIEAAGGVDRLPAGARLPELLGAYGGIHNSRLRADSPLRLKAVRLEAYR
                     WGGGFTDHGTFVRSWRQEL"
     gene            complement(2636..2923)
                     /locus_tag="AC159P_14055"
     CDS             complement(2636..2923)
                     /locus_tag="AC159P_14055"
                     /inference="COORDINATES: protein motif:HMM:NF017239.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HIR3696620.1"
                     /translation="MNGSNVDMDAPAPFDHVVFTEFDGGEGVLVDLNTKRYYQLNETA
                     ALVWRALEKGKNREEIVSEFTAQYEVSAEHAQASLDKILRRFQSYKLLRSS"
     gene            complement(2939..4015)
                     /locus_tag="AC159P_14060"
     CDS             complement(2939..4015)
                     /locus_tag="AC159P_14060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696621.1"
                     /translation="MKLRIYDAAPSAVCAPRAARVVAAEELKGWLPVDALVCAGEPYV
                     LWMDMRGVPLSEPFFQQTVERARGERPARTELLTDIGALVALEKISDSLPPTGFIFHA
                     SRCGSTLVSNACGAVRDTLVFSEPSAVDKLLGHFLTRADGAEFRTRLWRIFLRAAVSA
                     LGQRRAGQERGLVIKFACCGTLQLGLVKKLWPEVPRVFVYRDPSEIMVSNLNNVPDWM
                     RVAEQPEHTAAVVGVGPSEITRMGPEEFCARALGRFYDAAAEHADARTMLLAYEHINA
                     ETLAGVVRFFGAAPRPAEIEEIGRVSRLYSKDPARRRAFGGDGDAKRTGATPLIREMA
                     ERWAREPYERLRQKAPTDDGSPRP"
     gene            complement(4038..5117)
                     /locus_tag="AC159P_14065"
     CDS             complement(4038..5117)
                     /locus_tag="AC159P_14065"
                     /inference="COORDINATES: protein motif:HMM:NF015983.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HIR3696622.1"
                     /translation="MRTNTTAHDDDDYGPRRLSVELTNICNLHCSYCLRDEDALYHTP
                     ANFFPVELFRRVLGEAREAAGITHVMLTGGEPTLHPRFKEILEAAGREELKVSFVTNG
                     WHFERVWPVLQALSGVVTHVAFSIDGATREAHDRWRGEGSFVRLVRAFTRCRVGGLPF
                     AVKMGVRRDTLPQLEEVALFAARMGAESLYFDHVMPTSPSVEDESALDLEERAEAEQE
                     IATLARIFKMRIGLNVGYYYTEPTPPCSALAGVSFNVDYRGRLSLCCNMSGFRGAVGE
                     GDVVADLNEEDFSTAHARLRLVADAQLERRRAALAHHVADGTQPDLHTGSPCLFCLRS
                     FGKTPWSTDAGGGNVRRSLPLAMSV"
     gene            complement(5129..>5582)
                     /locus_tag="AC159P_14070"
     CDS             complement(5129..>5582)
                     /locus_tag="AC159P_14070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696623.1"
                     /translation="DDVMLLSESGGGVEACGLRRDFAVTARTLAASGLAASHAAARAA
                     FDPDKLRLPPADIFPAGHVETCAPTALFFPVVTGEAESRVEELSQAEAMARLIKMCPW
                     ATYDACAAPSHLGLLARLARQCAAHAVYAGTDVLRDSGLAARLIGPRL"
BASE COUNT          928 a         1975 c         1856 g          823 t
ORIGIN      
        1 gcccttcagc tcgttcagcg cgcggagcgc gtcccggttg aacgggtcaa ctccgaggcc
       61 gtagaatgtg tccgtgcgaa agccgacgac gccgcccgcg ccgacgacgc ccgccgcgcg
      121 cgcacgcgcc tcctgaccgt cagggatgac catcactttc ctccgccgcc gatgttacca
      181 aatcgcggcg gcgacgttca cacagccgag atgagaggac gactccacag tctgtcggga
      241 aggctcgcgc aggccgcgcg cttcgtcgcg cgcgagcctt tgtccggcct cctcatcgcg
      301 cgcatggccg cgtgggtcgc ggcgacttcg ctcctcctga acctgctgcc gttcgagaaa
      361 gcgatggtca tcatcacgcc cctgcggcgg cgcgagtccg tccgagaccc cgcggagacg
      421 gcggagacgc aggcccgcct ctcgcgcctc ctagacctcc tgctcgccac ggacttctgg
      481 ctctacacgc cgacctgctg gaagcgcgcg cccgtgctct accgcttcct cgcgctgagc
      541 ggcatcagga cgcgcgtcgt cttcggcgtg cgccgcaaag agtcgggcgc cctcgacggc
      601 cacgcttggc tcgaagccga cggcgagccg ctgctcgaaa gaaccgcccc cgactacacc
      661 gtcaccttca gctaccccac ctgaacaagg cagaagtcgg aaggcagaag gcggaaggcg
      721 gactggaagc agcgtgcttc tcatcatcga ctcgatgaga agcacagtgc atctagtctt
      781 cacttccgac ttctgccttg tttatcatcg gttcctcgcc gcgattcgat agccgagcca
      841 cgcgaaaggt ctcagcggct tgctctccag cagcgcggcg aggaatgtgt gccccgacgc
      901 ggcgagcacg cgggcgagcg cgcggctgcc gccgtagacc ctcccgattc cgtccaccca
      961 gtgcatctgc gccttcgtcc acctcgccac ttccgttggc aactccgtct cgattgactg
     1021 gtacggctgc gcttcgaagg gccgccgcgc gaaatgccgc agacgctcga cccagcgggc
     1081 gcagaagccg cagtcgccgt cccacaacag cgtctcgcgc gcgacgcact cctccacgcg
     1141 cacacacggg aaactttcgg gcacgccccg gaactccccc caccaccgcg cgagacgttt
     1201 tatcagcggc gcttgcgaga cgcgccgcgc gaccgcgggc cagtccacga agaccgcgta
     1261 catcgccagg tggaacggga agaagaagtt catcagaaag agcgtggcga cgtggaagac
     1321 caagccgaac ccgaggatca gccggcgcgc gcgccgcagg aaaatcgcca gcgggaacag
     1381 cagctcccac gcgaggacga gcgcgaccgc cgccgtcgtc aaggggcctc gatactccgc
     1441 gagccgaaac gcgtaggcga atcgcgcgtc gtgcaggttg tcgagcgagt ggctgacggc
     1501 gagcgcgggc aggctgtccg ggccgaagta ttcgaggccc gccacgcgca gcttgacgag
     1561 cgccgaactg aagtacgccc acgccaccag cgccctcatc aacagcaccg ggtagccgta
     1621 cttcacctcg ccgcggcgct ccgtgttcga gtccggcagg ctgtccacgg agaacgcgtc
     1681 gccggacggc gtgaaggcga ggacgacgag cacgtagacg gccagcatct cgtcatggtt
     1741 gaagtggccg aagctgcgga gcagcccctc gtaaaagagc accagcgcgg cggcgcactt
     1801 cgtcgtgagc ggtgtcaggt agccgaccgc gccgaacagc aaagacgcga ccagcagcgc
     1861 cttcagcgcc ggcatcccct gcggcgtgag cagccgctcg tagaactgcc aggagaagat
     1921 ttgcatcgcg ccggtcgggc gcatgacggt cgcgggcagg cgcgagaggg ctgcgaagtc
     1981 cgtgagcagg atcgagagca ggaaaaagcc gcacacgacg gcgcgcagca tgccgagcgc
     2041 gccggcagtc gcggggacga gacgggctag agttcctggc gccaagatcg gacgaatgtt
     2101 ccgtggtcgg tgaagccgcc gccccacctg taggcttcga gccgcacggc cttcaggcgc
     2161 agcggagaat ccgcgcgcag gcgtgaatta tgaatcccgc cgtaagcgcc gagcagctcg
     2221 ggcagccgcg cgcccgcggg cagtctgtcc acgccgcccg ccgcttcgat catcgcggcg
     2281 ttcgaggggt gcggcgagcg tatcctgaaa ccgccgttct ccgcggtcgc cccgaacatc
     2341 ccccagacgc gcccgccgag cgcgtccgtc aactcgacgg cgcgcaggtc aacgtccgtg
     2401 cccgcctgcg tctcgccgcg cagcacgtag tacgtgcgac cgcgggccac ttcggacggc
     2461 gaagtcatga ccgtccacgc ggcgaacggg tacaggttgc gcaacgcggc gaacgaccac
     2521 aggagcggcg cgaacagaaa gaccgtcagc agcgcgtagc gcggcaggta gcgccgccct
     2581 tcgtcccgac acgacccgtc ttccatcaac ggcctcgctc ccttcgcgcc cggctctagc
     2641 tcgaccgcag cagtttgtag gattggaacc gccgcaggat tttatcgagg ctcgcctgcg
     2701 cgtgctccgc cgacacctcg tactgcgccg tgaactcgga gacgatctcc tcgcggttct
     2761 tccccttttc gagcgcgcgc cagacgagcg ccgccgtctc gttcaactgg taataccgct
     2821 tcgtgttcag atcgaccagc acgccctcgc cgccgtcgaa ctccgtgaag accacgtggt
     2881 cgaaaggcgc gggcgcgtcc atgtcaacgt tgcttccgtt catcattcct ctcgcttatc
     2941 aagggcgcgg gcttccgtca tccgtcggtg ctttttgccg cagccgttcg tagggttctc
     3001 tcgcccaacg ctccgccatc tctcttatca gcggcgtggc gccggtgcgc ttggcgtcgc
     3061 cgtcgccgcc gaacgcgcgc cgccgcgcgg ggtctttgga gtatagacga gagacgcgcc
     3121 cgatctcttc gatttccgca gggcgcggcg cggcgccgaa aaagcggacg acgcccgcca
     3181 gcgtctcggc gttgatgtgc tcgtaggcga gcagcatcgt ccgcgcgtcg gcgtgctccg
     3241 ccgccgcatc gtagaatctc ccgagcgccc gcgcgcagaa ctcctccggc cccatgcgcg
     3301 tgatctcgct cggcccgacg ccgacgacag ccgccgtgtg ctccggctgc tccgcgacgc
     3361 gcatccagtc gggcacgttg ttcaggttcg agaccatgat ctccgaaggg tcgcggtaga
     3421 cgaagacccg cggcacctcg ggccacagtt ttttgacaag ccccaactgg agcgtcccgc
     3481 agcaggcgaa cttgatgacg aggccgcgct cttggcctgc gcgccgctgc ccgagcgcgg
     3541 agaccgcggc ccgcaaaaaa atccgccaca gtcgcgtccg gaattccgcg ccgtcggctc
     3601 tcgtcaaaaa gtgcccgagc agtttatcga cggcggacgg ctccgagaag acgagcgtgt
     3661 cgcgcacggc gccgcaggcg ttcgagacca gcgtcgagcc gcagcgcgac gcgtggaaga
     3721 tgaagcccgt cggcggcagg ctgtcggata ttttttcgag cgcgaccagc gcgccgatgt
     3781 cggtcaaaag ctccgtgcgc gccgggcgct ctccccttgc gcgctcgacc gtctgttgga
     3841 agaaaggctc cgacagcggg acgccccgca tgtccatcca caagacgtaa ggctccccgg
     3901 cgcacaccag cgcgtcaacc ggcagccacc ccttcaactc ttccgccgcg acgacgcgcg
     3961 cggcgcgggg cgcgcacacg gcggaaggcg cggcgtcgta aatccgcaac ttcatgcggg
     4021 gccttccgga aagtcggtca cacactcatc gccaacggca gggagcgccg cacgttgcct
     4081 ccgcccgcgt cggtgctcca gggggttttg ccgaacgacc tgaggcagaa gaggcacggc
     4141 gagcctgtgt gaaggtcggg ctgcgttcca tcggccacgt ggtgcgcgag tgccgcgcgc
     4201 cgccgctcaa gctgcgcgtc ggcgacgagg cgcaggcgcg cgtgggccgt cgagaagtct
     4261 tcctcgttga ggtccgcgac cacgtcgcct tcgccgacgg cgccgcggaa gccggacatg
     4321 ttgcagcaga gcgagagccg cccgcggtag tccacgttga agcttacgcc cgcgagcgcc
     4381 gagcagggcg gcgtcggctc cgtgtagtag tagccgacgt tgaggccgat ccgcatcttg
     4441 aagatgcgcg cgagcgtggc gatctcctgc tcggcctcgg cgcgctcttc gaggtcgagc
     4501 gccgactcgt cctcgaccga cggggaggtc ggcatcacgt ggtcgaagta gagcgactcg
     4561 gcgcccatgc gtgcggcgaa gagcgcgacc tcttcgagct gcgggagcgt gtcgcgccgc
     4621 acccccatct tgaccgcgaa cggcagaccg cccacgcggc agcgcgtgaa ggcgcgcacc
     4681 agacggacga aggagccttc gccgcgccag cggtcgtgcg cctcgcgcgt cgcgccgtcg
     4741 atgctgaagg cgacgtgcgt gacgacgccg ctcaacgctt gcaggacggg ccacacgcgc
     4801 tcgaagtgcc agccgttggt gacgaagctc accttcaact cttcgcggcc cgcggcctcc
     4861 agaatctctt tgaagcgtgg atgaagggtc ggctccccgc ccgtcagcat gacgtgcgtg
     4921 atgcctgccg cctcgcgcgc ctcgccgagg acgcggcgga acagctcgac ggggaagaag
     4981 ttcgcgggcg tgtggtagag cgcgtcctcg tcgcgcaggc agtagctaca gtgcaggttg
     5041 cagatgttcg tcagctcgac gctcaagcgg cgcgggccgt aatcgtcgtc gtcgtgcgcc
     5101 gtcgtgttgg ttcgcataag ggtctcgact acaggcgcgg gccgatgagg cgcgcggcca
     5161 gccccgagtc gcggagcacg tccgtgccgg cgtagaccgc gtgggccgcg cactgccgcg
     5221 cgagccgcgc gagcagaccg aggtgactcg gcgccgcgca cgcgtcgtac gtggcccacg
     5281 ggcacatctt gatgagacgc gccatcgcct cggcctgcga caactcttcg acgcggctct
     5341 cggcctcgcc cgtcaccacc gggaagaaga gggcggtcgg cgcgcacgtc tcgacgtggc
     5401 ccgcggggaa gatgtccgcg ggcggcagac gcagcttgtc cgggtcgaag gccgcgcgcg
     5461 cggcggcgtg actcgccgcg aggccgctcg cggcgagcgt cctcgcggtc acggcgaagt
     5521 cgcggcgcag gccgcacgcc tcgacgccgc cgccgctctc gctgaggagc atcacgtcgt
     5581 cc
//
LOCUS       DBNGOH010000726         1982 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2307697, whole genome shotgun sequence.
ACCESSION   DBNGOH010000726 DBNGOH010000000
VERSION     DBNGOH010000726.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1982)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1982)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1982
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2307697"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..501
                     /locus_tag="AC159P_14075"
     CDS             <1..501
                     /locus_tag="AC159P_14075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006098365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HIR3696624.1"
                     /translation="PLLRSGLALAGANAGQSAGGEDGILTALEASALDLWGTKLVVLS
                     ACETGVGEVLAGEGVYGLRRALVLAGSESQVMSLWQVSDEATRDLMVSYYKRLLAGEG
                     RTEALRQVQLEMLREGEGSRGKPKVLLGSAAAAEKADRSHPYYWAAFIQSGDWRPIGG
                     PAAPLQ"
     gene            749..>1982
                     /locus_tag="AC159P_14080"
     CDS             749..>1982
                     /locus_tag="AC159P_14080"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024258.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HIR3696625.1"
                     /translation="MIKFLHLSDVHLGCRRYNLEERTKDFFRSWYDVIDKYAVPNRVD
                     FVLVAGDFFDRRNIDPQTLNHALAGLQRLKDEGIPVVAIEGNHDRRDAVSEYSWMRSL
                     SRAGYIHLLEPGADEDSPISMTPWDEATREGSYIDIKGARIYGSHWYGTSAAAAMSML
                     ADALRRARSEDRFNILMLHTDVEGQLNRPIPALPVEKLKELRALVDYVALGHTHKRFE
                     IENWAFNPGSLEACSIDEYREERGVFLVEVDDRGQVTATFSRDYTQRPFQQLWFDVSG
                     AEDPEAVRAGVIEKVEREARRHDPDSEDPAPIIEISLRGHLGFKNSLLDLPAIREEAR
                     ELTGALHIMLKNQSVPVEYAVAAGLDADTSRQERERRIVEDLIARDNRFRDHAREMAE
                     LVLEAKRRALGDDTPADIL"
BASE COUNT          344 a          701 c          634 g          303 t
ORIGIN      
        1 ccgctcctgc gttcgggtct ggcgctggcc ggggcgaacg cggggcagag cgcgggcggc
       61 gaggacggca tcctgacggc gctcgaagcc tccgcgctcg acctgtgggg gacgaagctc
      121 gtcgtgctct cggcctgcga gaccggcgtc ggcgaggttc tcgccggcga gggcgtctac
      181 gggctgcggc gcgcgctcgt gctcgccggc tcggagagcc aggtgatgag tctgtggcag
      241 gtctcggacg aggcgacgcg cgacctgatg gtcagttact acaagcggct actggcgggc
      301 gaggggcgca ccgaggcgct gcggcaggtg caactggaga tgctcagaga aggggagggc
      361 agccgaggca aaccgaaagt cctcctcggc tcggcagccg cggcggagaa ggccgaccgc
      421 agtcacccct actactgggc cgccttcatc cagtcgggag actggcgccc catcggcggg
      481 ccggcggcac ctcttcagta atcatgatcc cgcgctgaag gcccacgcct gcggtgaatt
      541 accacatccc gccgaaaacg gcccggcaat ccgccgtggc ggaaaagttt gtcgcgcgac
      601 agttattcgt accgccctct tctcatcacg gtttccggtt gaacgttcac ggctttcgcc
      661 gccgatggcg tataatgtcg gcgccggagt gaagatttat gtccgcccct attcagcctt
      721 ggaacacagc tttgttaacg cgcgcgcgat gattaaattc ctgcacctgt cggacgttca
      781 cctgggctgc cgccgctaca acctcgaaga acgcacgaag gacttcttcc gcagttggta
      841 cgacgtgatt gataaatacg ccgtccccaa ccgcgtggac ttcgtgctcg tcgcgggcga
      901 cttcttcgac cgccgcaaca tcgacccgca gactttgaac cacgcgctcg ccggtctcca
      961 gcgactcaaa gacgagggca tcccggtcgt cgccatcgag ggcaaccacg accgccgcga
     1021 cgccgtctcc gagtacagtt ggatgcgctc gctctctcgc gccggctaca tccatctgct
     1081 cgaacccggc gcggacgagg acagccccat cagcatgacg ccgtgggacg aggcgacgcg
     1141 cgaaggctcc tacatcgaca tcaagggcgc gcgcatctac ggctcgcact ggtacggcac
     1201 gagcgccgcc gccgccatgt cgatgctcgc ggacgcgctg cggcgcgcgc gctcggaaga
     1261 ccgcttcaac atcctcatgc tccacaccga tgtcgagggc cagctcaacc gcccgatccc
     1321 ggcgctcccg gtcgagaagc tgaaggagct gcgcgcgctc gtcgattacg tcgcgctcgg
     1381 ccatacgcac aagcgcttcg agatcgagaa ctgggccttc aaccccggct ccctcgaagc
     1441 ctgctccatc gacgagtacc gcgaggagcg cggcgtcttc ctcgtcgagg tggacgaccg
     1501 cgggcaggtc acggccacct tctcgcgcga ctacacgcag cgccccttcc agcagctctg
     1561 gttcgacgtg tccggcgccg aagaccccga ggcggtgcgc gcgggcgtca tcgagaaggt
     1621 cgaacgcgag gcgcgccggc acgaccccga ttcggaagac cccgcgccga tcatcgagat
     1681 cagcttgcgc ggccacctcg gcttcaagaa ctcgctgctc gacctcccgg ccatccgcga
     1741 ggaggcgcgc gagctgacgg gcgcgctcca catcatgctc aagaaccaga gcgtgccggt
     1801 cgagtacgcg gtcgccgccg gcctcgacgc tgacacctcg cggcaggagc gcgagcgccg
     1861 catcgtcgaa gacctcatcg cgcgcgacaa ccgcttccgc gaccacgcgc gcgagatggc
     1921 cgaactcgtc ctcgaagcca agcgccgcgc cctcggcgac gacacccccg ccgacatcct
     1981 cg
//
LOCUS       DBNGOH010000727         2529 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3041585, whole genome shotgun sequence.
ACCESSION   DBNGOH010000727 DBNGOH010000000
VERSION     DBNGOH010000727.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2529)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2529)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2529
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3041585"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..863)
                     /locus_tag="AC159P_14085"
     CDS             complement(<1..863)
                     /locus_tag="AC159P_14085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3696626.1"
                     /translation="MPSPDKPRGGDSIADAVHSTMEPISLSENEGDQSTGASASGAAI
                     AASHEGLGGRIGRQRWVICALLFFATTVNYIDRLVFGLLGPELQKPELFGWTNKDYTD
                     IVFWFEVSYAIGLVSAGRVLDKVGTRLGYAVALIFWSIAAMLHAVAGWFAALVGISTV
                     ASFSIARFLLGLGEAGNFPAAIKTVAEWFPKKERALATGIFNAGSNVGAIGAPIIVPI
                     IYTYMGWQWAFILTGAIGLVWIIFWFALYHTPEKHPRITKEELAYIQSDPPDPAVKIA
                     WVSLLPHRQTWA"
     gene            complement(929..2050)
                     /locus_tag="AC159P_14090"
     CDS             complement(929..2050)
                     /locus_tag="AC159P_14090"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012766.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HIR3696627.1"
                     /translation="MEMKTELEEKTERVVRFLAEEGLGGVLLGAQHNFAWLTGGGRSG
                     IDLSREPGAGSLLVRADGRRFVVANNIEAARLLAEELDGYHWEPVEFDWTEEKANPSL
                     PFERATALLEDGAGLGSDLPAGGARVVEGSFARLRYQLTEQEIERLRSLGQDAGRAIG
                     EVARGLEPGLTEREVARRASDALAAVGAHSVVTLVAADERLKRYRHPVPTEARWEKVL
                     MIVVCARRGGLIASLTRIVSAGAMPDELRRRTEAAARVNAALYAATRPGAMGGELFDV
                     AARAYAAEGFAGEERLHHQGGATGYRTRDWVAHPRSEEKVQARQAFAWNPSITGTKTE
                     ETCLAFEDRVEVVTASPDWPAIPVEVAERSYVLPGVLPL"
     gene            complement(2275..>2529)
                     /locus_tag="AC159P_14095"
     CDS             complement(2275..>2529)
                     /locus_tag="AC159P_14095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HIR3696628.1"
                     /translation="VLAHSTHLRGMGEYDAATGVERPRVNVTLATRIPRERCERLSVG
                     YLDPDTVRPAEWQGREDEGILYVPKAGEMLYRVKPKAAAA"
BASE COUNT          375 a          908 c          847 g          399 t
ORIGIN      
        1 gcccatgtct gccggtgcgg cagcaggctc acccaggcga tcttcacggc ggggtcgggc
       61 gggtctgact ggatgtaggc cagctcttcc ttcgtgatgc gcgggtgctt ctccggcgtg
      121 tggtagaggg cgaaccagaa gatgatccag acgaggccga tggcgcccgt caggatgaag
      181 gcccactgcc agcccatgta ggtgtagatg atcggcacga tgatcggcgc gccgatggcg
      241 ccgacgttcg agccggcgtt gaagatgccg gtcgccagcg cgcgctcctt cttcgggaac
      301 cactcggcca ccgtcttgat ggccgccggg aagttgcccg cctcgccgag gccgagcagg
      361 aagcgcgcga tgctgaagct ggccacggtg ctgatgccga cgagggcggc gaaccagccc
      421 gccaccgcgt ggagcatcgc ggcgatgctc cagaagatga gcgccaccgc gtagccgagg
      481 cgcgtcccca ccttgtcgag cacgcggccc gcgctcacca ggccgatggc gtaggagacc
      541 tcgaaccaga agacgatgtc ggtatagtcc ttgttcgtcc agccgaagag ctccggcttt
      601 tgcagctcgg ggccgagcag gccgaacacg agccggtcga tgtagttgac cgtcgtggcg
      661 aagaagagca gcgcgcagat cacccagcgc tggcgaccga tgcgcccgcc gagaccttcg
      721 tgcgacgccg cgatggccgc gcccgaagcg ctcgcgcccg tcgattgatc gccctcgttc
      781 tccgagagcg agatcggctc catggtggaa tgaaccgcgt ccgcgattga gtccccgccg
      841 cggggcttgt ccggcgatgg cataacgggc tgttctcctt agtttagacg ttcagtcgcc
      901 gcttgctact tccggcgggc gattctcttc agagcggcaa cacgccgggc agcacgtagc
      961 tgcgctccgc gacctcgacc gggatcgcgg gccagtccgg cgaggccgtg acgacctcga
     1021 cgcggtcttc aaaggcgaga cacgtctctt ccgtcttcgt ccccgtgatc gaagggttcc
     1081 acgcgaacgc ctgccgcgcc tgcaccttct cctcgctccg cgggtgcgcg acccagtcgc
     1141 gtgtgcggta gccggtggcg ccgccctgat ggtgcagccg ctcctcgccc gcgaagccct
     1201 cggcggcgta ggcgcgcgcg gccacgtcga acaactcgcc gcccatcgcg cccggcctcg
     1261 tcgcggcgta cagcgcggcg ttcacgcgcg ccgccgcctc cgtgcgccga cgcagttcgt
     1321 ccggcatcgc gcccgccgag acgatgcgcg tcagcgacgc gatcagcccg ccgcgccgcg
     1381 cgcagacgac gatcatcaag accttctccc aacgcgcctc ggtcggcacc ggatggcggt
     1441 aacgcttgag ccgctcgtcc gcggcgacga gcgtgacgac cgagtgcgcg ccgaccgcgg
     1501 cgagcgcgtc cgaagccctg cgcgcgacct cgcgctccgt gagtcccggt tcaagtccgc
     1561 gcgcgacttc gccgatggcg cggcccgcgt cctgccccaa agagcggagg cgttcgatct
     1621 cctgctcggt cagttgatag cgcagacgtg cgaacgagcc ttcgaccacg cgcgcgccgc
     1681 ccgccggaag gtctgagcct aagccggccc cgtcttcgag cagcgccgtc gcgcgctcga
     1741 acgggagcga agggttggcc ttctcctccg tccagtcgaa ctcgaccggc tcccagtgat
     1801 acccgtccaa ctcttccgcg agcagccgcg ccgcctcgat gttgttggcg acgacgaacc
     1861 gccgcccgtc ggcgcggacg agcaacgaac ccgctcccgg ctcgcggctg aggtcgatgc
     1921 cgctgcggcc cccgcccgtc agccacgcga agttgtgctg cgcgccgagc agcacgccgc
     1981 ccaagccttc ctccgcaagg aagcgcacga cgcgctcggt cttctcttcg agttcggtct
     2041 tcatttccat ccgagatata agcggccacc gaccccgcgg cttcgccgcc tgatgtgcgg
     2101 tgaggctccg cctcaccgtt gacgtacttc tttagtccgg ctgaggctac gcctcagcgc
     2161 gggggcgtag cccctacata atcgaaggcg actgtagccg gaaaggcaga gcctttccgc
     2221 acatctggcg gcggagccgc cgaagaaccg tgcttgactt ccacgcctcg cgcgtcacgc
     2281 ggcggcggcc ttcggcttca cgcggtagag catctcgccc gccttcggga cgtagaggat
     2341 gccttcgtcc tcgcggccct gccactccgc gggacggacg gtgtcggggt cgaggtagcc
     2401 gacgctcagg cgctcgcagc gctcgcgcgg gatgcgcgtg gcgagcgtga cgttgacgcg
     2461 cgggcgctcg acgccggtcg cggcgtcgta ctcgcccatc ccgcgcaggt gcgtgctgtg
     2521 cgcgagcac
//
LOCUS       DBNGOH010000728         2805 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2123696, whole genome shotgun sequence.
ACCESSION   DBNGOH010000728 DBNGOH010000000
VERSION     DBNGOH010000728.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2805)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2805)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2805
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2123696"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..994
                     /locus_tag="AC159P_14100"
     CDS             <1..994
                     /locus_tag="AC159P_14100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3696629.1"
                     /translation="INALPTRELVSHIKRRGVDFEMTPDAESEIRGAGARPEIIEAAR
                     ANYRAPATTPAATATARPTPRPTGPSAAPVPAGPPLSKDAILELLRNGVPAERVERNV
                     EARGVNFTMTPEIAREITAAGGNRSLVGAITEKSTEAASSSGPGGGSVFDPGPNAGPT
                     YDDLIESASSAYLSRNLNGAIAYVQQAVQLDAQNPTAYQLLGLWALNEGQLPTAEQAL
                     RAALERNGAAPFKVAHDHDGSFSNYCMGSFFISKTGVSYVADDGRHTFEVLDSNIKEV
                     KKNGFVGAAIGAFHVKPVAKVRDNDGKERDNFNLAPFNGNKAVSDMIVRLVQAY"
     gene            1014..2144
                     /locus_tag="AC159P_14105"
     CDS             1014..2144
                     /locus_tag="AC159P_14105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696630.1"
                     /translation="MRRTSAAFILTRVAVGRALPVGLIVAASLLGVSCMKAGTSEVAV
                     TPNSNTANSSSSSNTANSSSSYDSGKYKELLDKGNEIEKLSLPVKLGPKAVIEGKVFF
                     VENTPARARNADYDQGVSDYRKAGNLEELESVIRIICAKGKFLGDYFREVSGTKQSVK
                     AYGIDCQVSLIDYTARAVVAQKTFSNNESGEAILERSVAGEQINPPPMGDMTSYINSF
                     SVDKVLPTMARLDEKELVRLPTTVDLNPDAAIRGKIKIVQKYEKGDLGSNLYSFIEGS
                     DYFSLPRERFADKPEELETLVKIVCVKGSPIGRVEKTAQYSSKCEVSLIDYKTLSVFA
                     RKTIENKTLDESPAAQSSTSKAWVVGPPKQEIESYLKSFPTS"
     gene            2245..>2805
                     /locus_tag="AC159P_14110"
     CDS             2245..>2805
                     /locus_tag="AC159P_14110"
                     /inference="COORDINATES: protein motif:HMM:NF024816.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3696631.1"
                     /translation="MKRAHRLSAGLLTLAVVCASLALAPAGGGQERDESDVHYTKGNE
                     LVRAGDYKNAVAAYRRALKLKPDSVHAHSGLGLAHLRLRQFKEAAEAFEKAVKLDPAY
                     AAGYNGLGDALYGLGRTADAIEQFRAAIRLSPDNPAAYAKLGRAYIDLEKYDEAVEWL
                     GKALRIRADYVDAHRYLGNVYLKQRNY"
BASE COUNT          592 a          942 c          850 g          421 t
ORIGIN      
        1 gatcaacgcc ctcccgaccc gggagttggt cagccacatc aagaggcgcg gcgttgactt
       61 cgagatgacg cccgacgccg agtccgagat acgcggcgcg ggcgcccggc ccgaaatcat
      121 cgaggcagcg cgcgccaact accgtgcccc ggccaccaca ccggcggcga ccgcaaccgc
      181 gcgcccgacg ccgcgcccga ccggcccctc tgcggcgccc gtccccgccg ggccgccgct
      241 ctcgaaggac gcgatcctcg aactgctccg caacggcgtc cccgccgagc gcgtcgagcg
      301 caacgtcgag gcgcgcggcg tcaacttcac gatgacgccc gagatcgccc gcgagatcac
      361 cgccgccggc ggcaaccgct cgctcgtcgg cgccatcacc gagaaatcga ccgaggccgc
      421 ctcttcctcc ggccccggcg gcggcagcgt cttcgacccc ggccccaacg ccgggccgac
      481 ctacgacgac ctcatcgagt cggcgagcag cgcctacctc tcgcgcaacc tcaacggcgc
      541 catcgcctac gtgcagcagg ccgtccagct cgacgcgcag aacccgaccg cctaccaact
      601 gctcggcctc tgggcgctca acgaaggcca gttgcccacc gccgagcagg ccctgcgcgc
      661 ggcgctcgaa cgcaacggcg cggccccctt caaggtcgcg cacgaccacg acggcagctt
      721 cagcaactac tgcatgggtt ccttcttcat ctcgaagacg ggcgtctctt acgtcgccga
      781 cgacgggcgg cacaccttcg aggtgctgga ctcgaacatc aaagaggtca agaagaacgg
      841 cttcgtcggc gcggccatcg gcgccttcca cgtcaagccc gtcgccaaag tccgcgacaa
      901 cgacggcaag gagcgcgaca acttcaacct cgcccccttc aacggcaaca aggccgtctc
      961 cgacatgatc gtccgcctgg tgcaggctta ctaacttgac gggagaccga accatgagaa
     1021 gaacatcggc ggcattcatt cttacgcggg ttgctgtcgg aagagcgctc ccggtgggcc
     1081 tcatcgtggc cgcatcgctc ctcggcgtct cctgcatgaa ggcggggacg agcgaagtgg
     1141 ccgtcacccc caactcgaat acggcgaact cgtcgtcctc ctcgaacacg gcgaactcgt
     1201 catcctctta cgacagcggt aagtacaagg agctactgga caaggggaat gaaatcgaga
     1261 agctgtctct gcccgtgaag ctcggcccga aagcggtgat cgaggggaaa gtctttttcg
     1321 tggaaaacac cccggcgcgg gcccgcaacg cagattacga ccagggggtc tccgattaca
     1381 ggaaggcggg gaatctggaa gagcttgagt cggtgattcg catcatctgc gcgaagggga
     1441 agtttctcgg cgactacttc agagaggtct cgggcactaa acagtcagtc aaagcctacg
     1501 gcatcgactg ccaggtttcg ctcatagatt acacggcgcg cgccgttgtg gcccaaaaga
     1561 ccttttccaa caacgagagc ggcgaagcga ttttggagag aagcgtggcg ggcgaacaaa
     1621 tcaatccacc ccctatgggc gacatgacca gctacatcaa cagcttctcc gtggataaag
     1681 tgttgccgac gatggctcgg ctggacgaaa aggagttggt caggcttccc acgaccgttg
     1741 acttgaaccc cgacgcggcc atcaggggca aaataaaaat cgtgcaaaag tacgagaagg
     1801 gtgatctcgg ctccaacctg tactccttca tcgaaggctc ggattatttc agcctccccc
     1861 gcgagaggtt cgccgacaag cccgaagagc tggagaccct tgtgaagatc gtctgcgtga
     1921 aaggcagccc gataggcaga gtcgaaaaaa ccgcgcagta ttcgagcaag tgcgaggttt
     1981 cgttgattga ttacaagact ctcagcgtgt tcgcccgaaa gaccatcgag aataaaacgt
     2041 tggacgaaag cccggcggcg caaagttcca cgagtaaagc ctgggtggtc ggcccgccca
     2101 agcaggagat agaaagttac ctgaaaagtt tcccgacctc ctgaaacgag ggcgacattg
     2161 aacgttctcc cggaatcgtt gcggctttgc ggcgcggcgg cgcgcggtcg gccccgcccg
     2221 gctcgccccg cgtgcgacaa aagtatgaag agagcacatc gactgagcgc cggcctgctg
     2281 acactggctg tggtctgcgc gtccctcgcc ctggcccccg cgggcggcgg gcaggagcgg
     2341 gacgagtcgg acgttcacta caccaaaggc aacgaactcg tccgggcggg cgactacaag
     2401 aatgccgtcg cggcctaccg gcgggcgttg aagctgaagc ccgactcggt gcacgcccac
     2461 agcggtctcg gcctcgcgca cctccgcctc aggcagttca aagaggccgc cgaggcgttc
     2521 gagaaggccg tcaagctcga ccccgcatac gcggcgggct acaacgggct gggcgacgcg
     2581 ctctacgggc tggggcgaac cgcggacgcc atcgagcagt tccgggcggc catccgcctg
     2641 tccccggaca accctgcggc ctacgcgaag ctgggccgcg cctacatcga cctcgaaaaa
     2701 tatgacgagg ccgtcgaatg gctcggcaaa gccctgcgca tcagggccga ctacgtggac
     2761 gcccaccgct atctggggaa cgtctacctg aaacaacgga actac
//
LOCUS       DBNGOH010000729         1909 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_5057880, whole genome shotgun sequence.
ACCESSION   DBNGOH010000729 DBNGOH010000000
VERSION     DBNGOH010000729.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1909)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1909)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1909
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_5057880"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..458
                     /locus_tag="AC159P_14115"
     CDS             <1..458
                     /locus_tag="AC159P_14115"
                     /inference="COORDINATES: protein motif:HMM:NF013936.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HIR3696632.1"
                     /translation="RDEMERITALTEKVVSKHGENHPELARVSALFRQLCDDLRPHML
                     KEENILFPYVKQLEQAAADGRPLPRAPFGTVGNPVRMMMFEHDTAGDLLRELRRAAAD
                     YAAPADACVSYLTLYEALEGFEQDLHQHIHLENNVLFPRAVELEKSSRR"
     gene            complement(658..1356)
                     /locus_tag="AC159P_14120"
     CDS             complement(658..1356)
                     /locus_tag="AC159P_14120"
                     /inference="COORDINATES: protein motif:HMM:NF012257.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HIR3696633.1"
                     /translation="MKTDKIAALGRTALFGELDEQSLHALAVRAIERRYARGEVLFLA
                     GEEARGLYVIVSGSVRAFRESLDGREQVIHVERAGATVAELPVFDDGPYPSTVAAEEA
                     TTVLFIDKRNVRRLSLEHPEIALAALKVLAGRLRRCAELVEALSLREVGQRLARFLLA
                     EARAGGTPAADGLQVELSHTNQQIAARVGSVREVVSRALTRLQHDGLIRLEGRLLTIP
                     DEQALASFAGEEEQ"
     gene            complement(1343..>1909)
                     /locus_tag="AC159P_14125"
     CDS             complement(1343..>1909)
                     /locus_tag="AC159P_14125"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014672639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase family protein"
                     /protein_id="HIR3696634.1"
                     /translation="VGLDSSEVGHPPVKFKAVFEKARAHGLPAVAHAGEEGPPDYIRQ
                     ALDELRVVRVDHGVRCLEDPELVARLVAEQVPLTVCPLSNVRLRVVGTMAEHPLKRML
                     EAGLCVTVNSDDPAYFGGYLTENLLAAHRALGIDGDGIRRLLRNSFRSSFLSDAEREK
                     LAVEVDSHFSAGVKTRAVRVEDRLDEDG"
BASE COUNT          315 a          667 c          625 g          302 t
ORIGIN      
        1 cgcgcgacga gatggagcgc atcaccgcgc tcacggagaa ggtcgtgtcg aaacacggcg
       61 agaaccatcc ggagctggcg cgcgtgagcg cgctcttccg gcagctctgc gacgacctgc
      121 gcccgcacat gctcaaggag gagaatattc tctttcccta cgtcaagcag ttggagcagg
      181 cggcggcgga cgggcggccc ctcccccgcg cgccgttcgg cacggtgggc aaccccgtcc
      241 gcatgatgat gttcgagcac gacacggcgg gcgacctttt gcgcgagctg cgccgcgccg
      301 ccgccgacta cgccgcgccc gccgacgcct gcgtcagcta cctgacgctc tacgaggcgc
      361 tcgaaggctt cgagcaggat ttgcaccagc acatccacct cgaaaacaac gtgctcttcc
      421 cgcgcgccgt cgagttggag aagagcagca ggcggtaggc ggtaggcagt aggcagtaaa
      481 ggcaggaagt cggaattcag aagtcagaag aaggaagagg cttgagagct aacgtctcaa
      541 gcctcttcgt tcgtcaagta tccgagatcg aggctctgga ctttacctct caagcctctt
      601 cgcccttctt gccttttact gcctactgcc taccgcctac tgcctactgc ccgctgccta
      661 ctgctcttcc tctccggcga atgacgcgag ggcttgctcg tcggggatgg tgaggaggcg
      721 gccttcgagc cggatcaggc cgtcgtgctg gaggcgcgtg agcgcgcgcg agacgacttc
      781 gcgcaccgac ccgacgcggg ccgcgatctg ttgattggtg tgggagagtt cgacctgtag
      841 gccgtccgcc gcgggcgtgc ccccggcgcg tgcctcggcg agcaggaacc gcgcgaggcg
      901 ctggccgacc tcgcgcaggg agagcgcttc gaccagttcg gcgcagcggc gcaagcgtcc
      961 ggcgagcacc ttgagcgcgg ccagagcgat ctccgggtgt tccagcgaga ggcggcgcac
     1021 gttgcgcttg tcgatgaaga gcacggtggt cgcctcctcg gcggcgacgg tcgaggggta
     1081 cgggccgtcg tcgaagacgg gcagctccgc cacggtcgcg ccggcccgct cgacgtggat
     1141 gacctgctcg cgcccgtcaa ggctttcgcg gaaggcgcgc accgagccgc tcacgatgac
     1201 gtagaggccg cgcgcctcct cgcccgccag aaagagcacc tcgccgcgcg cgtagcggcg
     1261 ctcgatggcg cggacggcca gcgcgtgcag gctctgttcg tccagctcgc cgaagagggc
     1321 cgtgcgcccg agtgccgcga tcttatccgt cttcatcgag cctatcttcg acccgcaccg
     1381 cgcgcgtttt caccccggcg ctgaaatgac tatccacttc gaccgcgagc ttctccctct
     1441 ccgcgtcact caaaaacgaa ctccggaagg agttcctgag cagccgccgt atcccgtctc
     1501 catcgatacc cagcgcacga tgcgcggcca gaaggttctc cgtcaggtag ccgccgaagt
     1561 aagccgggtc gtccgagttg accgtcacgc acagccccgc ctccagcatc cgcttgaggg
     1621 ggtgctcggc catcgtgccc acgacccgga gcctcacgtt cgagagcggg cagaccgtga
     1681 gcgggacctg ctcggcgacc agcctcgcga ccagctccgg gtcttcgaga caccgcacgc
     1741 cgtgatcgac ccgcacgacc cgcagctcgt ccagcgcctg ccggatgtag tccggcggcc
     1801 cctcctcgcc ggcatgggcc acagccggca ggccgtgcgc gcgcgccttc tcgaacaccg
     1861 ccttgaactt gacgggcggg tggccaacct cggacgagtc gaggccgac
//
LOCUS       DBNGOH010000730         3958 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4140333, whole genome shotgun sequence.
ACCESSION   DBNGOH010000730 DBNGOH010000000
VERSION     DBNGOH010000730.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3958)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3958)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3958
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4140333"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1014
                     /locus_tag="AC159P_14130"
     CDS             <1..1014
                     /locus_tag="AC159P_14130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696635.1"
                     /translation="ALSAGALHAAPFVLVLAAYVVARAAALGSLVGGYGAEHHLNFTH
                     SMIVSQLLRFPLRALFPALALRHATFLESRLLSPVLIITGVLVVILAALALSRARARK
                     TLAAFARRNTFLWTLAALFLAALLPAINLRIDVFTTHGERYLYFPSAFFCVALAHVLT
                     KSIRSGRRALVALSCILVFYAASLWLTNRHWAEAARISRAVVEDLSTQSAGNAVLVLN
                     LPDNLDGAHLFRNGLPEALRWFADGETGGDVRVLAWHGLRSPTGGAVLTSEGAGLFTL
                     RLSDEGDVFGRVNEGVAHVEVVERGARRLRLRLITNEEDGANPVDVFHFSGGRMVKVP
                     DVR"
     gene            complement(1118..1849)
                     /locus_tag="AC159P_14135"
     CDS             complement(1118..1849)
                     /locus_tag="AC159P_14135"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HIR3696636.1"
                     /translation="MVRPKRASSISTAFEDEARARGHRRVAGVDEVGRGALAGPVVAA
                     AVVLDPEVAFPAGLDDSKKLTRLQRERIAEELKRSALCYAVGVVSHEEIDAANILIAT
                     RRAMVEAVTKLDPCADFLLIDAVQLRESALPQRAIIGGDAVSASIAAASVIAKTYRDA
                     LMHDYDTRFPEYGFARHVGYGTRAHWAALAAHGCCPIHRRSFRGVLQTPPPAAVEETA
                     RVEDKHETGDADDAQGVARRNAESP"
     gene            complement(1977..2381)
                     /gene="rplS"
                     /locus_tag="AC159P_14140"
     CDS             complement(1977..2381)
                     /gene="rplS"
                     /locus_tag="AC159P_14140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HIR3696637.1"
                     /translation="MNRLDSIEKAQMREGIPEFQPGDTVRVHVRIKESETKERLQAFE
                     GVVIARKGGGIRETFTVRKTSFGVGVERVFPLHGPIVGKIELVRRGRVRRAKLYYLRN
                     LRGKAARIKELDTRQKSSGQAQGASASNKSGK"
     gene            complement(2496..3209)
                     /gene="trmD"
                     /locus_tag="AC159P_14145"
     CDS             complement(2496..3209)
                     /gene="trmD"
                     /locus_tag="AC159P_14145"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HIR3696638.1"
                     /translation="MRFDIITIFPDFFGGVFDYGIVRRARAAGLVETEAHDLRRWTHD
                     KHKIVDDRPFGGGDGMVLKAEPIFEAVESLAGAAEAEALRAAGKRVVLLSPQGRVLTQ
                     ALAAELAGESHVIVICGRYEGVDERVAERLVTDEVSIGDYVLSGGEPAAVVLVDAVVR
                     LIPGALGSETSAVNESFAEGLLDFPHYTRPPDLRGMRVPEVLLGGNHAEIARWRREAA
                     LSKTRRNRPDLLAAGEGEK"
     gene            complement(3278..3823)
                     /gene="rimM"
                     /locus_tag="AC159P_14150"
     CDS             complement(3278..3823)
                     /gene="rimM"
                     /locus_tag="AC159P_14150"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HIR3696639.1"
                     /translation="MSGREEIDLGGVELVAVAKAVRVRGLKGEVVCELLTDFPERFEW
                     VEELIGVAPSGARKMLALERHWLQKGRVVLKFVGYDTPEAAQGLVGWELTVPESEAVE
                     LEEGEYYDWQLVGCRVETVDNRSVGTVREVLHAGAAPVLVIRDERDRENLVPLAESIC
                     VEVDTEAKLIRVDAPEGLIEF"
     gene            complement(3823..3954)
                     /locus_tag="AC159P_14155"
     CDS             complement(3823..3954)
                     /locus_tag="AC159P_14155"
                     /inference="COORDINATES: protein motif:HMM:NF024483.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HIR3696640.1"
                     /translation="MRVAQEDMGKIIGRQGRTVKALRSLLHAAGQKHDRRYVLELVE"
BASE COUNT          613 a         1452 c         1205 g          688 t
ORIGIN      
        1 gccctctccg ccggggcgct acacgccgcg cccttcgtcc tcgtgctggc cgcctacgtc
       61 gtcgcgcgcg ccgccgcgct cggctcgctc gtcggcggct acggcgcgga gcaccacctc
      121 aacttcacgc actcgatgat cgtcagccag ctcctgcgct tccccttgcg cgccctcttc
      181 cccgcgctcg cgctccgcca cgccaccttc ctcgaatcgc gactgctctc gcccgtcctc
      241 atcatcacgg gcgtgctggt cgtcatcctt gccgccctcg ccctctcgcg cgcccgcgcg
      301 cggaagactc tcgcggcctt cgcgcgccgg aacactttcc tctggacact cgccgcgctc
      361 ttcctcgcgg cgctgctgcc cgcgatcaac ctgcgcatcg acgttttcac gacgcacggc
      421 gagcgttacc tctacttccc ttccgccttc ttctgcgtcg ccctcgcgca cgtgttgacg
      481 aaatcgatac gaagcggacg ccgcgcgctc gtcgccctct cgtgcatcct cgtcttctac
      541 gcggcttcgc tgtggctgac gaaccgccac tgggccgagg ccgcgcgcat ctcacgcgcc
      601 gtcgtcgaag acctgtcaac gcagtccgca ggcaacgccg tcctcgtcct caacctgccc
      661 gacaacctcg acggcgcgca cctcttccgc aacggcctgc ccgaagcgct gcgctggttc
      721 gcggacggcg agacgggcgg cgacgtgcgc gtcctcgcct ggcacggtct gcgctcccca
      781 accggcggcg ctgtgttaac cagcgaaggc gcaggactct tcacactacg actctcggac
      841 gaaggcgacg tgttcggtcg ggttaacgag ggcgtcgcac acgtcgaggt cgtcgagcgc
      901 ggcgcgcgcc gactgcgact ccggctcatt acgaatgaag aggacggcgc gaatccagtc
      961 gatgtttttc acttcagcgg cggcaggatg gtgaaagtgc cggacgttcg gtgagattgt
     1021 gggggcaggg cttgtccctg cccgtcaact ttagtttgct tcgatgtcgg atgaatacaa
     1081 agccgcgggc cacggacaag ccctgcccct acagtcatca cggagattca gcgttccttc
     1141 gcgcaacgcc ttgcgcgtcg tccgcgtcgc ccgtctcgtg tttatcttcg acgcgcgccg
     1201 tctcttcgac cgcggcgggc ggcggtgttt gcaggacgcc gcggaagctg cggcggtgga
     1261 tgggacagca gccgtgcgcc gccagcgcgg cccagtgcgc gcgcgtgccg tagccgacgt
     1321 ggcgcgcgaa gccgtactct gggaaccgcg tgtcgtagtc gtgcatcagc gcgtcgcggt
     1381 aggtcttggc gatgacggag gcggcggcga tggaggccga gacggcgtca ccgccgatga
     1441 tggcccgctg cggcagcgcg gattcgcgca actgcaccgc gtcgatcaaa agaaaatcgg
     1501 cgcacgggtc gagcttcgta acggcctcga ccatcgcgcg ccgcgtggcg atgagaatgt
     1561 tggcggcgtc gatctcttca tgcgagacga cgccgaccgc gtaacagagc gccgaccgct
     1621 tcaactcctc cgcgatgcgc tcgcgctgaa gccgcgtcag cttcttcgag tcgtcgagac
     1681 cggcggggaa agcaacctcc gggtcgagga cgaccgccgc cgcgaccacc ggcccggcca
     1741 gagcaccccg gcccacctcg tccacgcccg ccacccgtcg gtgcccgcgg gcgcgcgcct
     1801 cgtcctcgaa cgccgtcgag atcgaagatg cgcgcttcgg acgaaccata aaagccgtaa
     1861 gccgtgaacc gtaaaccgtg acaagtgaaa agccgccacg agtaaaccgc gaaccgtgat
     1921 aagcagaacg cggttctgac ttatcacggt ttacggttca cggcttacgg cttttgctac
     1981 ttgccgctct tgttcgaggc ggacgcgccc tgcgcctggc cgctgctctt ctggcgcgtg
     2041 tcgagttcct tgatgcgtgc agccttgccg cgcaggttgc gcaggtagta gagcttggcg
     2101 cggcggacgc gcccgcgccg cacgagttcg atcttgccga cgatggggcc gtgcagcggg
     2161 aagacgcgct cgacgccgac gccgaagctc gtcttgcgca cggtgaaggt ctcgcggatg
     2221 ccgccgcctt tgcgcgcgat gacgacgccc tcgaacgcct gaaggcgctc cttcgtctcc
     2281 gactccttga tgcgcacgtg gacgcgcacc gtgtcgcccg gctgaaactc cgggatgccc
     2341 tcgcgcatct gcgccttctc aatactgtcc aagcgattca tgattcacct cacgatagct
     2401 gtcagccttc agctttcagc actcagcctc ttgtcttcag cgaaagctga ccgccgaacg
     2461 ccgacagctt ccaatcctaa atcgaaaatc caaactcact tctcgccttc gcccgcggcg
     2521 agcaggtcgg ggcggttgcg ccgcgtcttc gagagcgcgg cctcgcggcg ccagcgcgcg
     2581 atctccgcgt ggttgccgcc gagcagaact tccggcacgc gcattccgcg aaggtcgggc
     2641 gggcgcgtgt agtgcgggaa gtcgagcagc ccttcggcga acgactcgtt gaccgccgag
     2701 gtctcgctcc ccaacgcgcc cggtatgagc cgcacgaccg catctaccag cacgaccgcc
     2761 gccggctcgc cgcccgacag cacgtagtcg ccgatggaga cttcgtcagt cacgagcctc
     2821 tcggcgacgc gctcgtccac gccctcgtag cgcccgcaga tgacgatgac gtgcgactcg
     2881 ccggccagct cagccgccag cgcttgcgtc agcacgcgcc cctgcggcga gagcagtacc
     2941 acgcgcttgc ccgccgcgcg caatgcctcg gcttccgcag cgcccgccag agattccacc
     3001 gcctcaaaga tcggctcggc cttcaagacc atcccgtcgc cgccgccgaa cggcctgtcg
     3061 tccacgatct tgtgcttgtc gtgcgtccag cggcgcaggt cgtgcgcctc ggtctcgacc
     3121 agccccgccg cgcgcgcgcg ccgcacgatc ccgtaatcga acaccccgcc gaagaagtcg
     3181 gggaagatcg tgatgatgtc aaaacgcatt gcggaattcg gattgcggat tgcggattta
     3241 taaagacgag acagctttta aatccgcaat ccgcaatcta aaattcaatg agtccttccg
     3301 gcgcgtccac gcggatcagt ttcgcttcgg tgtccacctc gacgcagatg ctctcggcga
     3361 gcgggacgag gttctccctg tcgcgctcgt cgcggatgac gaggacgggg gccgcgcccg
     3421 cgtgcaagac ctcgcgcacg gtgccgacgc ttcggttgtc aacggtctcg acgcggcagc
     3481 ccacgagctg ccagtcgtag tactcgccct cttccagctc gaccgcctcg gactcgggca
     3541 ccgtcagctc ccagccgacc aggccttgcg ccgcctcggg cgtgtcgtag cccacgaact
     3601 tcaggacgac gcgccccttc tgcaaccagt ggcgttcgag cgcgagcatc tttcgcgcgc
     3661 ccgacggcgc gacgccgatc aactcctcga cccactcgaa gcgctcgggg aagtccgtca
     3721 gcagctcgca gaccacttcg cccttcaggc cccgcacccg cacggccttc gccacggcga
     3781 ccagctcgac gccgccgagg tcgatctctt cgcgcccgct cattactcga cgagttcgag
     3841 gacgtagcgc cggtcgtgct tctgccccgc cgcgtgcagg agcgagcgca gggccttcac
     3901 cgtgcggccc tgccgcccga tgatcttgcc catgtcttcc tgcgccacgc gcacctcg
//
LOCUS       DBNGOH010000731         2016 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_108920, whole genome shotgun sequence.
ACCESSION   DBNGOH010000731 DBNGOH010000000
VERSION     DBNGOH010000731.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2016)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2016)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2016
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_108920"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..287
                     /locus_tag="AC159P_14160"
     CDS             <1..287
                     /locus_tag="AC159P_14160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HIR3696641.1"
                     /translation="WAKIEIAGERGSYEVPPHREDYAGIVISLARQETPDTTPYDDPE
                     IVFRLHKKHHVGLIAASRDLGRVEHLLTDYARRFSTDFLASMPSPDKPTS"
     gene            complement(448..1161)
                     /locus_tag="AC159P_14165"
     CDS             complement(448..1161)
                     /locus_tag="AC159P_14165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HIR3696642.1"
                     /translation="MKSMLLGLGLFCAALLAAACGGSGETSSRTRDGQTAPPSSTTST
                     PQDLPKIVAFGDSLTAGYGLAPAESYPSLLQKKLDADGFKYEVVNAGVSGDTSAAGLR
                     RIDWSLDAGNVRFVILELGANDFLRGQPVAETRKNLSEIIKRAKGRGAVVLLAGMYTP
                     TNAGREYQQEIHEAFRALATEHEVALIPFFLEGVAGNASLNLDDRVHPNAEGTKIVAD
                     TVYRHLKPLLEKQQQGGRG"
     gene            1160..1891
                     /locus_tag="AC159P_14170"
     CDS             1160..1891
                     /locus_tag="AC159P_14170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3696643.1"
                     /translation="MIELYKVSKTVTSGAEQLTILHSLDLTIPDGQFVAVVGPSGSGK
                     STLLGLVAGLDAPTEGVIKIDGEDITRMNEDALATLRGRKLGFVFQSFHLIPSMTAFE
                     NVLAPMEIAGRRGARERARQLLEDTGLTARAHHYPSQLSGGEQQRVAVARAFANDPSI
                     LLADEPTGNLDSRNGAHVFELMLEMNRRRGATLVLVTHERELAEMADRQIHLRDGRVV
                     AGMKKDESAGVENDPRREEMEGAAR"
BASE COUNT          344 a          674 c          655 g          343 t
ORIGIN      
        1 agtgggcgaa gatcgagatc gcgggcgagc gcggcagcta cgaagtgccg ccgcaccgcg
       61 aagactacgc cggcatcgtc atctcgctcg cgcggcagga gacgcccgac accacgcctt
      121 acgacgaccc cgagatcgtc ttccgcctcc acaagaaaca ccacgtcggc ctcatcgccg
      181 cctcgcgcga tctcgggcgc gtcgagcacc tgctcacgga ctacgcgcgc cgcttctcga
      241 cggacttcct cgcctccatg ccttcgcccg acaagccgac gagctaaagc ggtgaagggg
      301 aaaaggggaa gaggcgaaga ggcgaagagg gaggataaga agcagcgtgc gtctgatcct
      361 cagtcgatga ctgaaaacag cgtggctctc atcattcaat gatgagagcc acgctgcttc
      421 ttgtctccct tttcgcctct tcccctttta acctcttccc ccttgttgtt gcttttcgag
      481 cagcggcttc agatggcgat agaccgtgtc ggcgacgatc ttcgtccctt cggcgttggg
      541 gtgcacgcgg tcgtctaaat tcagcgaggc gttgccggcg acgccttcga ggaagaaggg
      601 gatgagcgcc acttcgtgct cggtggcgag cgcgcggaag gcttcgtgta tctcctgctg
      661 gtactcgcgc cccgcgttgg tcggcgtgta catccccgcg aggaggacga cggcgccgcg
      721 ccccttcgcg cgtttgatga tctccgagag gttcttccgc gtttcggcga cgggctggcc
      781 gcgcaggaag tcgttggcgc cgagttcgag aatcacgaag cgcacgttgc ccgcgtcgag
      841 cgaccagtcg atgcggcgca gtcccgccgc ggaggtgtcg cccgaaacac cggcgttgac
      901 gacctcgtac ttgaagccgt cggcgtcgag cttcttttgc agcagcgagg ggtagctctc
      961 ggctggcgcg aggccgtagc ccgcggttaa gctgtccccg aaggcgacga tcttcggcag
     1021 gtcctgcggc gtggaagtcg tcgatgaagg cggcgctgtc tggccgtctc ttgtgcgcga
     1081 agaggtctcg cccgagccgc cgcaggccgc cgcgagcaac gcggcgcaaa aaagaccgag
     1141 tccgagaagc attgatttca tgatcgaatt atacaaggtc tcgaagaccg tcacctccgg
     1201 cgcggagcag ttgacgatcc ttcactcgct cgacctgacg attcccgacg gccagttcgt
     1261 cgccgtcgtc ggcccctcgg gcagcggcaa atcgacgctg ctcggcctcg tcgccggtct
     1321 cgacgccccg accgagggcg tcatcaaaat cgacggcgaa gacatcacgc gcatgaacga
     1381 ggacgcgctg gccacgctgc gcgggcgcaa gctcggcttc gtcttccagt cgttccacct
     1441 catcccctcg atgacggcct tcgagaacgt gctcgcgccg atggagatcg cgggccgccg
     1501 cggcgcgcgc gaacgcgcgc ggcaactcct cgaagacacg gggctgacgg cgcgcgcgca
     1561 ccactacccc tcgcaactct cgggcggcga gcagcagcgc gtcgccgtcg cccgcgcctt
     1621 cgccaacgac ccctccatcc tgctcgcgga cgagccgacc ggcaacctcg actcgcgcaa
     1681 cggcgcgcac gtcttcgagc tgatgctgga gatgaaccgc cgccgcggcg cgacgctcgt
     1741 cctcgtcacg cacgagcgcg agctggccga gatggccgac cgccagattc acctgcgcga
     1801 cggtcgcgtc gtggccggca tgaaaaagga tgagagcgcc ggtgtcgaga acgacccgcg
     1861 gcgggaagag atggaaggag cggcgcgttg aagttcgtcc tgaacatggc gcggcgcgag
     1921 ctgcgcgcct cgtggcggcg gctgctcttc ttcttcctgt gcgtcggcat cggcgtgggg
     1981 agcatcgtcg ctttgcgctc gatcatccgc aacatc
//
LOCUS       DBNGOH010000732         2105 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3042235, whole genome shotgun sequence.
ACCESSION   DBNGOH010000732 DBNGOH010000000
VERSION     DBNGOH010000732.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2105)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2105)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2105
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3042235"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..759)
                     /locus_tag="AC159P_14175"
     CDS             complement(<1..759)
                     /locus_tag="AC159P_14175"
                     /inference="COORDINATES: protein motif:HMM:NF012787.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HIR3696644.1"
                     /translation="MFETLARTAAFFALALAAPAASLAQQTAPAQTPPTAQTTPAPQP
                     AQTPQTPPQAQASPAPQAGHEYAPLLEQTVNYKDWTFKSLKDGKDVNLREWAKGKKLV
                     LVVYFAPWCGNWKMEAPIVARLYNEYKQHGFDVVAVSEYAAPADTEKYFEGVGGAPYT
                     VVVESEGREARDKTTHYGYRQACGDSRRWGSPFNVFLEPAKLNKSGDVLTEKAWVVGG
                     ELIEKDVERFVRERLGIKEQGAAAEQCKDEPQKTA"
     gene            complement(859..>2105)
                     /locus_tag="AC159P_14180"
     CDS             complement(859..>2105)
                     /locus_tag="AC159P_14180"
                     /inference="COORDINATES: protein motif:HMM:NF014713.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HIR3696645.1"
                     /translation="ITVVLAFVAGLASFLSPCVLPLVPGYISLISGVSVEHLKGAGGS
                     RANAKRAVVMNSLAFNAGVSVVFILLGATAGWIGNVLFSNVWVRVVGGLVIIVFGLHL
                     MGVLKIAAFYRDTRKFSDEKPRGALGSLTLGMAFAAGWTPCIGPVLGGIIGLAATSGG
                     WKSGLLLSSFYAAGLAVPFLLTGLLLNQFLGFYTWFRRHLHKVEVFSGVLLIVMGLMV
                     ASNSLTWVASVAARYLPNAEGLVQSWGGGGATPTAGTASTQPAAASNASFEPAPEVEL
                     KTLDGEAFRLADARGRVVLLNFWATWCVPCRSEIPTLNAMNSELGPRGFTVVGVTTDD
                     TPELVREFQKDIVKQDYTVVFGERAAAAKFAVGVLPTTFIIDREGRIRHQIIGEKSRA
                     EFEAKIKPLLDEAPAATARRAN"
BASE COUNT          353 a          757 c          663 g          332 t
ORIGIN      
        1 cgcggttttc tgcggctcgt ccttgcactg ctcggccgcc gcgccctgct ccttgatgcc
       61 gaggcgctcg cgcacgaagc gctccacgtc cttctcgatc agctcgccgc cgacgaccca
      121 cgccttctcc gtcagcacgt cgcccgactt gttgagcttc gccggttcga ggaagacgtt
      181 gaagggcgaa ccccagcggc gcgagtcgcc acacgcctga cggtagccgt agtgcgtcgt
      241 cttgtcgcgc gcctcgcgcc cctcggactc gacgacgacc gtgtagggcg cgccgcccac
      301 gccctcgaaa tacttctcgg tgtcggcggg cgcggcgtac tcgctgacgg ccaccacgtc
      361 gaagccgtgc tgcttgtact cgttgtagag acgcgccact atcggcgcct ccatcttcca
      421 gttgccgcac cacggcgcga agtagacgac gagcacgagc ttcttcccct tcgcccactc
      481 gcgcaggttc acgtccttgc cgtccttcag gctcttgaat gtccaatcct tgtagttgac
      541 cgtctgctcc aagagcggcg cgtattcgtg gcccgcctgc ggcgccgggg aagcctgcgc
      601 ctgcggcggc gtctgcggcg tctgggcggg ctgcggcgcc ggtgtcgttt gggcggtagg
      661 cggcgtctgc gccggcgcgg tctgttgcgc gagagaagcg gcgggcgcgg cgagcgcgag
      721 ggcgaagaag gcggcggtgc gcgcgagagt ttcgaacatc ttgactgcct ctctttcgtt
      781 gacgacgaag ggcgtgcgtg aaacccgtca gtgaaaatca aacccgcctg acgacgaacg
      841 aacccgtccg tggtgaaact aattcgcccg gcgcgcggtg gccgcaggcg cctcgtcgag
      901 cagcggcttg atcttggcct cgaactcggc gcgcgacttc tcgccgatga tttgatgacg
      961 gatgcgcccc tcgcggtcga tgatgaaggt ggtcggcagc acgccgaccg cgaacttcgc
     1021 cgccgccgcg cgctcgccga agacgaccgt gtagtcctgc ttcacgatgt ccttctgaaa
     1081 ctcgcggacg agttccggcg tgtcgtcggt cgtcacgccg acgaccgtga agccgcgcgg
     1141 ccccagctcg ctgttcatcg cgttcaaggt cggaatttcg ctacggcacg ggacgcacca
     1201 cgtcgcccag aagttgagca gcaccacgcg cccgcgcgcg tccgcgagcc ggaaagcctc
     1261 gccgtcgagc gtcttcaact ccacctccgg cgccggctcg aacgaggcgt tacttgccgc
     1321 cgccggttgc gtcgaagccg tgcccgcggt cggcgtcgca ccgccgccgc cccaagattg
     1381 aacaagtccc tcggcgttcg gaaggtaacg cgccgcgacg ctcgccaccc acgtcaggct
     1441 gttgctcgcc accatcaagc ccatcacgat cagcagcacg cccgagaaga cttcgacctt
     1501 gtgcaggtgg cggcggaacc atgtgtagaa gccgaggaac tggttgagca gcagccccgt
     1561 cagcaggaac ggcacggcga gtcccgccgc gtagaacgaa gagagcagca gcccgctctt
     1621 ccacccgccg ctcgtcgcgg cgaggccgat gatgccgccg agcacggggc cgatgcacgg
     1681 cgtccaaccg gcggcgaacg ccatccccag cgtcaaagac ccgagcgcgc cgcgcggctt
     1741 ctcgtccgag aacttccgcg tgtcgcggta gaacgccgcg atcttcagca cgcccatcag
     1801 gtgcagcccg aagacgatga tgacgaggcc gccgacgacc ctcacccaca cgttcgagaa
     1861 gagcacgttg ccgatccacc cggcggtcgc gccgagcagg atgaagacca ccgacacgcc
     1921 cgcgttgaac gccagcgagt tcatcaccac cgcgcgcttc gcgttcgcgc gcgaaccgcc
     1981 cgcgcccttc aggtgctcca cgctcacgcc cgagatcagc gagatgtaac ccggcaccag
     2041 cggcaacacg cacggactca agaacgaagc cagccccgcc acgaacgcca ggaccaccgt
     2101 aatgt
//
LOCUS       DBNGOH010000733         4951 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1760008, whole genome shotgun sequence.
ACCESSION   DBNGOH010000733 DBNGOH010000000
VERSION     DBNGOH010000733.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4951)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4951)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4951
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1760008"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1415
                     /locus_tag="AC159P_14185"
     CDS             <1..1415
                     /locus_tag="AC159P_14185"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HIR3696646.1"
                     /translation="SERYPMLGPLASVTGSRALVALPLIVKGRTIGAMGLSFPREQSF
                     TEDDRAFMLALAQQCAQALERARLYETEQRLRAEAEAANRLKDEFLATVSHELRTPLT
                     AIVGWSSMLRANVLDEESKARAVEIIERNAKAQTQIVEDLLDVSRIITGKLTLDTRAA
                     ELSAVVEAALESVRPAAAKKGLTIRSEVEPGVGAVWGDPARLEQVMWNLLANAVKFTP
                     DGGTVSVRLSRAGRCVRVAVADTGQGIAPEFLPFVFHRFRQADGSTTRTHGGLGLGLA
                     IVRQLVELHGGTVSAESAGEGRGATFTVELPVMAAGPVGAEPSADSSAVGDSVDEPSA
                     ALDAATTLADLRVLLVDDEPDARLLLTTVLEQHGARVLAVASAPQALAALASFRPDII
                     VSDIGMPGEDGYSLLRKVRSHAPADGGRVPAVALTAYARAEDRIRALRAGYQVHVAKP
                     INPAEFVAVVGALAGVTARQ"
     gene            complement(1566..3638)
                     /locus_tag="AC159P_14190"
     CDS             complement(1566..3638)
                     /locus_tag="AC159P_14190"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014679817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0009311 - oligosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosidase family protein"
                     /protein_id="HIR3696647.1"
                     /translation="MNVSRRRALSRVAGLLACVLIWQPAARAQETRWRLEADGGITWN
                     VEQGQAHQDHVEMSGRRVSAIVTYGVDDKGRLFVSRHVVFPMLRFAPNKTRDHLALVF
                     ADDAAPRFLLNRAAPRNEVVTSVRQLGVVRIESVLGREREVSLTRTIFPSVDKPLVFE
                     KYTFTNRSRQDVTIEVEETEKVVRTNPARGVYGGYVATSRVLGYGERVIRPRESATFA
                     LVIGARKTGEPAPAVDVEAEERARRERVAGFLSKLQLETPDRVLDAAFAFAKIRAAES
                     IYETGGGLMHGPGGGAYYAAIWANDQAEYANPFFAFLGDATANESAINSFRHFARYMN
                     PEYRPIPSSIISEGADFWHGAKDRGDMAMIAYGAGRFALAYGKRETAAELWPLVEWSL
                     EYSRRKVNAEGVVASDSDELENRFPAGKANLNTSALYYDALNSAVMLGRELGKPEPLL
                     KDYAERARHVRAAIEKHFGANVEGFATYRYYDKRDLIGHAKFAAYAERPDVLRAWISI
                     PLTVGIYERKEATIAALFSPRLWTPDGLATEAGDKTFWDRSTLYGLRGVFAAGATEKA
                     LEYLTYYSRRRLLGDHVPYPVEAYPEGNQRHLSAESALYCRVYTEGVFGMRPTGFDSF
                     DTTPRLPDRWDSMKLKNIHAFGRVFDLSVSRAGERLKVEIVRGGKTVRSHVIEKGETL
                     RVDLARGS"
     gene            3825..3971
                     /locus_tag="AC159P_14195"
     CDS             3825..3971
                     /locus_tag="AC159P_14195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696648.1"
                     /translation="MATIGSGFGGLGGARLKPVEYVFGFPRAAEARAAESVNAATTGE
                     ANYA"
     gene            3964..>4951
                     /locus_tag="AC159P_14200"
     CDS             3964..>4951
                     /locus_tag="AC159P_14200"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019474.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR3696649.1"
                     /translation="MPEALATRSERLQGAAARALSRLPAWLQVRLSGQPPVVIDEQTL
                     DPQVQLLLAIMRWLDRGGRGPSSPAESRARLRQEMRAVSAPKTNVGAVRDFEIPGGAG
                     PLPVRHYAPPADVDTRALLVYLHGGGFVIGDLDTHDEVCRLLCRHAGTHVMSVDYRLA
                     PEHPFPAAVEDTLAALRWAQANASSLGAEAARVSVGGDSAGGNLATVAARLAAHEGSP
                     PAAQLLIYPTTDAQAHYASKELFDEGFFLSRADCDTFYRRYTGGTGFGHEDPRVSPLR
                     APSDQLRLPPVLVITAGFDVLRDEGEAYAAKLREAGTTVRARRFEALGHGFVN"
BASE COUNT          726 a         1786 c         1676 g          763 t
ORIGIN      
        1 actccgagcg ctacccgatg ctcgggccgc tcgcctccgt cacgggcagc cgcgcgctcg
       61 tcgcgctccc gctcatcgtc aagggccgca ccatcggcgc gatgggcctc agcttcccgc
      121 gggagcagag cttcacggaa gacgaccgcg ccttcatgct cgcgctcgcg cagcagtgcg
      181 cgcaggcgct tgagcgcgcg cgcctctacg agaccgagca gcgcctgcgc gccgaggccg
      241 aggccgcgaa ccgcctcaag gacgagttcc tcgcgacggt ctcgcacgag ctgcggacgc
      301 cgctgacagc catcgtcggc tggtcgagca tgctgcgcgc gaacgtgctc gacgaagagt
      361 cgaaggcgcg cgccgtcgag atcatcgagc gcaacgcgaa ggcgcagacg cagatcgtcg
      421 aagacctgct cgacgtgtcg cgcatcatca ccggcaagct cacgctcgac acgcgcgccg
      481 ccgagctgtc cgcggtcgtc gaggcggcgc tcgaatccgt ccgccccgcg gcggcgaaga
      541 agggactcac gatcaggtcg gaagtcgagc cgggcgtcgg cgccgtgtgg ggcgacccgg
      601 cgcggctcga acaggtgatg tggaacctgc tggcgaacgc cgtcaagttc acgcccgacg
      661 ggggcacggt gagcgtgcgc ctctcgcgcg cgggccggtg cgtgcgcgtc gccgtcgcgg
      721 acacggggca gggcatcgcg cccgagttcc tgcccttcgt cttccaccgc ttccggcagg
      781 ccgacggctc gaccacacgc acgcacggcg gcctcggcct cggcctcgcc atcgtccgcc
      841 agctcgtcga gctgcacggc ggcacggtca gcgccgagag cgcgggcgag gggcgcggcg
      901 cgaccttcac cgtggagctg ccggtcatgg ccgccgggcc ggtcggtgcg gaaccttcgg
      961 cggactcgtc ggcggtcggc gactccgtgg acgagccgtc ggcggcgctc gacgccgcca
     1021 cgacgctggc cgacctgcgg gttcttttag tggacgacga gccggacgcg cggctcctgc
     1081 tgacgaccgt gctggagcag cacggcgcgc gcgtgctcgc cgtcgcgtcc gccccgcagg
     1141 cgctcgccgc gctggcctcg ttccggcccg acatcatcgt cagcgacatc ggcatgcccg
     1201 gcgaagacgg ctactcgctg ctgcgcaaag tgcgctcgca cgcgcccgcg gacggcgggc
     1261 gcgtgcccgc cgtggccctg accgcctacg cccgcgcgga agaccgcata cgcgcgctcc
     1321 gcgccggcta tcaggtgcac gtggcgaagc ccatcaaccc cgccgaattc gtcgcggtgg
     1381 tcggcgcgct cgcgggcgtc accgcgcgcc agtgatgcgg tacgttgtga tgactgtagg
     1441 ggcagggctt gtccctgccc gccgtcacgc gttcatccaa cgtcgaatct atcgaagccg
     1501 cgggccgcgg acttgccctg cccctacagt cttcgccctc gttctacagc cgactgaaac
     1561 ttgctctaag acccccgcgc caaatcaacc cgcagcgtct cgcccttttc gatgacgtgt
     1621 gaccggacgg tcttccctcc cctgacgatc tccaccttca gccgctcgcc cgcgcgcgag
     1681 accgagagat cgaagaccct gccgaaggcg tggatgttct tcagcttcat gctgtcccaa
     1741 cggtcgggca ggcgcggcgt ggtgtcgaac gagtcgaagc ctgtggggcg catgccgaac
     1801 acgccctcgg tgtagacgcg gcagtagagc gcgctctcgg cggagaggtg gcgctggttg
     1861 ccttccgggt acgcctcgac cgggtacggc acgtggtcgc ccagcagacg gcggcgcgaa
     1921 tagtaagtca ggtactccag agccttctcg gtcgcgcccg cggcgaacac gccgcgcagc
     1981 ccgtagagcg tcgagcgatc ccagaaggtc ttgtcgcccg cctcggtcgc caacccgtcc
     2041 ggcgtccaca gccgcggcga gaagagcgcg gcgatggtgg cctccttgcg ctcgtagatg
     2101 ccgaccgtca gcgggatcga aatccacgcg cgcagtacgt cgggcctctc cgcgtaagcg
     2161 gcgaacttcg cgtggccgat aaggtctcgc ttatcgtaat aacggtacgt cgcgaagcct
     2221 tcgacgttgg cgccgaagtg cttctcgatg gcggcgcgga cgtggcgcgc gcgctcggcg
     2281 taatccttga gcagcggctc cggcttgccc agctctctgc cgagcatgac cgccgagttc
     2341 agcgcgtcgt agtagagcgc ggacgtgttg aggttcgcct tgcccgccgg gaagcggttc
     2401 tccagttcgt cgctgtcaga ggcgaccacg ccctccgcgt tcaccttccg gcggctgtat
     2461 tcgagagacc actcgacgag cggccacagc tcggcggcgg tctcccgctt cccgtaagcg
     2521 agcgcgaagc gccccgcgcc gtaggcgatc atcgccatgt cgccccgatc cttcgcgccg
     2581 tgccagaagt ccgcgccctc cgagatgatg gaactcggga tcggcctgta ctccgggttc
     2641 atgtagcgcg cgaagtggcg gaacgagttg atggccgact cgttcgccgt cgcgtcgccg
     2701 aggaaggcga agaaggggtt ggcgtactcg gcctgatcgt tcgcccagat ggccgcgtag
     2761 taggcgccgc cgccggggcc gtgcatcagg ccgcctccgg tttcgtagat gctctcggcg
     2821 gccctgatct tggcgaaggc aaaggcggcg tcgagcacgc ggtcgggcgt ttcgagttgc
     2881 agcttcgaga ggaagcccgc cacgcgttcc ctgcgcgcgc gctcctctgc ctcgacatcg
     2941 acggcgggcg ccggctcgcc cgtcttccgc gcgccgatga ccagcgcgaa ggtcgcgctc
     3001 tcccgcggcc tgatgacgcg ctcgccgtag ccgaggactc gcgacgtggc gacgtagccg
     3061 ccgtagacgc cgcgcgcagg gttcgtgcgg acgaccttct ccgtctcctc gacctcgatt
     3121 gtcacgtcct gccgcgagcg gttggtgaag gtgtacttct cgaagaccag cggcttatcg
     3181 accgaaggga agatggtgcg cgtcagggac acttccctct cccgccccaa cacgctctcg
     3241 atgcgcacga cgcccagttg gcggacgctc gtgacgacct cgttgcgcgg cgcggcgcgg
     3301 ttcaggagga agcgcggcgc ggcgtcgtcg gcgaagacga gcgcgaggtg gtcgcgcgtc
     3361 ttgttcggcg cgaagcgcag catgggaaag acgacatgac ggctgacgaa gagcctgccc
     3421 ttgtcgtcca cgccgtaggt gacgatggcg gagacgcgcc tgccgctcat ctcgacgtga
     3481 tcctgatgcg cctggccctg ttcgacgttc caagtgatgc cgccgtccgc ctccagccgc
     3541 cagcgcgtct cctgcgcgcg cgccgcgggc tgccagataa ggacgcacgc gagcaacccg
     3601 gcgaccctcg acaacgccct ccgccgtgag acgttcatcg tcaacgaatc ctcctcgcga
     3661 ttcttcccgg cctgtccccc gacccgacgc ggcgccgaaa accgggaacg cgttgttatc
     3721 gataacaagc ctcagaatac cacattttcc actccgcgtg ggcgaggtcg tgattcgggg
     3781 ctaggccggg gcggacgagt tggattatat ctatcggcag gcgcgtggcg accatcggca
     3841 gcggcttcgg cggactgggc ggcgcgcgcc tgaagcccgt ggagtacgtc ttcggttttc
     3901 cgcgcgcagc ggaggcgcgg gccgcggagt ccgtgaacgc ggcgacgacc ggagaagcga
     3961 actatgcctg aagcgttggc gacccgttcg gaacgtttgc agggcgcggc ggcgcgcgcg
     4021 ctcagccgtc tgcccgcgtg gctgcaagtg cgcctctccg gccagccgcc ggtcgtcatc
     4081 gacgaacaga cgctcgaccc gcaggtgcaa cttcttctag ccatcatgcg ctggctcgac
     4141 cgcggcggac gcggcccttc ctcgcccgcc gagtcgcgcg cacgtctccg gcaggagatg
     4201 cgcgccgtct ccgccccgaa gacgaacgtc ggcgccgtgc gcgacttcga gattccgggc
     4261 ggggcagggc cgctgcccgt ccgtcattac gcgccgcccg ccgacgtgga cacgcgcgca
     4321 ctgctcgtct acctgcacgg cggcggcttc gtcatcggcg acctcgacac gcacgacgaa
     4381 gtgtgccgcc tgctgtgccg ccacgcgggc acgcacgtca tgagcgtcga ttacaggctc
     4441 gcccccgaac acccgttccc ggcggcggtc gaagacacgc tcgccgcgct ccgctgggcg
     4501 caggcgaacg cctcctcgct cggcgccgaa gcggcgcgcg tctcggtcgg cggcgacagc
     4561 gcgggcggca acctcgccac cgtcgccgcg cgcctcgccg cgcacgaagg ctcgccgccc
     4621 gccgcgcagt tgctcatcta cccgaccacc gacgcgcagg cgcactacgc ctcgaaggag
     4681 cttttcgatg aaggcttctt cctcagccgc gcggactgcg acaccttcta ccggcgttac
     4741 acgggcggca ccggcttcgg ccacgaagac ccgcgcgtct ctccgttgcg cgccccgtca
     4801 gaccaactga ggctgccgcc tgtcctcgtc atcacggcgg gcttcgacgt gctgcgcgac
     4861 gaaggcgaag cctacgccgc gaagctccgc gaggccggga caaccgtccg tgcgcgccgc
     4921 ttcgaagccc tcgggcacgg cttcgtcaac a
//
LOCUS       DBNGOH010000734         2654 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_476595, whole genome shotgun sequence.
ACCESSION   DBNGOH010000734 DBNGOH010000000
VERSION     DBNGOH010000734.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2654)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2654)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2654
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_476595"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1450
                     /locus_tag="AC159P_14205"
     CDS             <1..1450
                     /locus_tag="AC159P_14205"
                     /inference="COORDINATES: protein motif:HMM:NF017044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HIR3696650.1"
                     /translation="LHACRARVALPARRRLPLFGLLLFACALLAAHLPVAGQNAPEPR
                     REQLLNGLRLLIISRPGDPEVLLRARVHSGAAFDLAGREGLTALLGDALFDANTREFV
                     REDLDGRLEVSTGYDYIDITLAGRASDFERFVDLVRNGLTNTQLSPDVVGKLREARIK
                     AAREVAPSPSAVADRAAAARLFGKHPYGRSIEGTPESLARVERADLMLTRERFLNPNN
                     TTLVIIGGVEPRTVMRLLRQNLGAWTKSDRVIPSTFRQPEAPDARTLVIDSPGAAGVE
                     VRLAVRGLARADRDAPAVHALVALARARWAAALPELGSGKFFVRHDARTESGIFLMGA
                     TLPSSASAAKALDAARKVLAELAGAAPAAAEFETARQAASASLSEGAGGGNDLIADEL
                     LAEHTYKTRAVTRAENALRLAALTPAEAQRVAARLFLHTPVASVAVGEAARLTEELAR
                     AGGVEVFGAPGAAPSTQAAPTLPPPAVRLKRP"
     gene            1511..2254
                     /gene="ubiE"
                     /locus_tag="AC159P_14210"
     CDS             1511..2254
                     /gene="ubiE"
                     /locus_tag="AC159P_14210"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013557989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052624 - 2-phytyl-1,4-naphthoquinone
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0042372 - phylloquinone biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HIR3696651.1"
                     /translation="MTRSQPPQESASRDEHARRVREMFAGIANRYDLLNHLLSGNVDR
                     RWRRLVAEKLRPALDADSTVLDVACGTGDLSLVIAEACGARVVGVDFCRPMLDIAARK
                     SAGVSRTIPFVEGDALRLPFADEKFDAATIAFGLRNLADVAGGLGELRRILKPGGRLV
                     VLEFSRPVVPGFRALFQFYFSRVLPRVGGLVSGSRGAYEYLPDSVSKFPDQARLASLM
                     KEVGFENVDYRNLTGGIAALHSGTRRHTV"
BASE COUNT          398 a          959 c          837 g          460 t
ORIGIN      
        1 actccacgcc tgccgcgcgc gcgtcgccct acctgcgcgc cgtcgcctgc cgctcttcgg
       61 cctcctgctg ttcgcctgcg cgctgctcgc tgcccacctg ccggtcgcgg gccagaacgc
      121 gcccgagccg cgccgcgagc aactgctgaa cggactccgc ctgctcatca tcagccgacc
      181 gggcgacccc gaagtgctgc tgcgggcgcg ggttcacagc ggcgccgctt tcgacctcgc
      241 gggcagagaa gggctgacgg cgctcctggg cgacgcgctc ttcgacgcga acacgcgcga
      301 gttcgtccgc gaagacctcg acgggcgcct cgaagtctcg accgggtacg actacatcga
      361 catcacactg gcgggccgcg cgagcgattt cgagcgcttc gtcgatctcg tcaggaacgg
      421 gctgacgaac acgcagctct cgcccgacgt ggtcggcaag ctgcgtgagg cacgcatcaa
      481 ggccgcgcgc gaggtcgccc ccagcccctc agcggtcgcc gaccgcgccg ccgccgcgcg
      541 cctcttcggc aaacaccctt acggcaggag catcgaaggc acgcccgagt cgctcgcccg
      601 cgtcgagcgc gccgacctga tgctcacgcg cgagcgcttc ctcaacccga acaacacgac
      661 cctcgtcatc atcggcggcg tcgagccgcg cacggtcatg cgtctgctgc gccagaacct
      721 cggcgcgtgg acgaagagcg accgcgtcat cccctcgacc ttccgccagc ccgaggcgcc
      781 cgacgcgcgc acgctcgtca tcgacagccc cggcgctgcg ggcgtcgaag tgcgtctcgc
      841 cgtgcgcggt ctcgcccgcg ccgaccgcga cgctccggca gtccacgcgc tcgtcgcgct
      901 cgcccgcgcg cgctgggccg cggccctgcc cgaactcggg agcggtaaat tcttcgtccg
      961 ccacgacgcg cgcacggagt ccggcatctt cctgatgggg gccacgctcc cctcttcggc
     1021 gtccgcggcg aaggcgctcg acgccgcgcg caaggtgctc gccgaactcg cgggcgcggc
     1081 ccccgccgcc gccgagttcg agacggcgag gcaggccgcc tccgcttcgc tgagcgaagg
     1141 cgcgggcggc ggcaacgact tgatcgcgga cgaactgctc gccgagcaca cctacaagac
     1201 gcgcgccgtc acgcgcgcag agaacgcgct gcgcctcgcc gcgctcacgc ctgccgaggc
     1261 gcagcgcgtc gcggcgcggc tcttcctcca cacgccggtc gcgtcggtcg ccgtgggcga
     1321 ggccgcgcgc ctcaccgaag agctggcgcg cgcgggcggc gtcgaggttt tcggcgcgcc
     1381 gggcgcggcc ccctcgacgc aggcggcgcc cacgttgccg ccgcccgccg tgcgactcaa
     1441 acgcccgtag ccaattcgca aacaaatcac aaatattccg caaccaaccg ttttcttaat
     1501 ccaataaagt ttgacccgca gtcaacctcc tcaagagagc gcgtcgagag acgaacacgc
     1561 gcgccgcgtg cgcgagatgt tcgcgggcat cgccaatcgc tacgacctct tgaaccacct
     1621 gctctcgggg aacgttgacc gccgctggcg ccgcctcgtc gccgagaaac ttcggcccgc
     1681 gctcgacgcg gattcgacgg tgctcgacgt ggcctgcggg acgggcgatc tgtcactcgt
     1741 catcgccgaa gcgtgcggcg cgcgcgtggt cggcgtggac ttctgccgac cgatgctcga
     1801 catcgccgcg cgcaagtccg caggcgtgtc gcgcacgata cctttcgtcg aaggcgacgc
     1861 gttgcgtctg cccttcgcgg acgagaagtt cgacgcggcc accatcgcct tcggcctgcg
     1921 gaacctcgcg gacgtggcgg gcggtctcgg ggagttaagg cggattttga aaccgggcgg
     1981 gcgcctcgtc gtcctcgaat tctcaaggcc cgtcgtgccg ggttttcgcg cgctctttca
     2041 gttctacttt tcgcgggtgc tgccgcgcgt cggcggcctc gtcagcggtt cgcgcggggc
     2101 ttacgaatat ttgcccgact cggtctcgaa gtttccggat caggcgcggc tcgcgtcgct
     2161 gatgaaggag gtcgggttcg agaacgtcga ttaccggaac ctgaccgggg gcatcgcggc
     2221 cctgcattcg ggcacgcgcc gccacacagt ttgatacaag ctcggttgct tcaattcatt
     2281 gaagcgacca aatcgtagct cgatagactc tcgcccgctt gttggttgtc ggggccgaca
     2341 ctcgggattg aaagtttgtc gggctacgtt tttttccgaa gggggaaaag ggtaaaaggg
     2401 ggagagggaa aaagggaaaa gacaaaaact tttcgcctct tcccttcttg acctcttcgc
     2461 ccttcttgct tttcgcctct tcccctttta cccttttccc ccttcctttg ttatcatcct
     2521 ccgcgtaatt caatccttca ggccgtcaac cccgttcttg atgaagcacc gtcttctcga
     2581 catcctgcgg agactgctga tcctgctgct cgtcgtgtgg acggtggtgt ctctggtgac
     2641 gctgctgatc gagc
//
LOCUS       DBNGOH010000735         4308 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_109377, whole genome shotgun sequence.
ACCESSION   DBNGOH010000735 DBNGOH010000000
VERSION     DBNGOH010000735.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4308)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4308)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4308
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_109377"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1229
                     /locus_tag="AC159P_14215"
     CDS             <1..1229
                     /locus_tag="AC159P_14215"
                     /inference="COORDINATES: protein motif:HMM:NF024180.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HIR3696652.1"
                     /translation="LRKEGEPLWVDLGAVESVDGDLARLLAALKCPQTVDDIRKCPSV
                     AEVKRLARAADERVMRPVRKLLGDTRHVFVSPDGALNLLPFAALVDENGKYLVETYSL
                     TYLTSGRDLLRLQAGAGSREPPLVVADPAYDGARAFPTPTARIQAGEESARRSGEMGA
                     MRFRPLPGTAAEAKALGAALPGVRVLTRERATEAALKQVTAPRVLHVATHGFFLPDQP
                     QQTAAEDARGITLSGGGGPPVPARVENPLLRSGLALEGANQRRGAGGEDGILTALEAA
                     ALDLWGTKLVVLSACETGVGEVKNGEGVYGLRRALVLAGSESLVMSLWQVSDEATKDL
                     MVSYYKRLLAGGGRTEALRQVQLEVLRGGGRAAGGQGRCLFCEPGKAGAPRDLSHPYY
                     WAAFIQSGDWRGMGMR"
     gene            complement(1303..1725)
                     /locus_tag="AC159P_14220"
     CDS             complement(1303..1725)
                     /locus_tag="AC159P_14220"
                     /inference="COORDINATES: protein motif:HMM:NF039194.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5681 domain-containing protein"
                     /protein_id="HIR3696653.1"
                     /translation="MQGNNREVGYGRPPVHTRFKKGRSGNPKGRPRSAAISDAVRAVL
                     AESPPGGEGADVESYADAIARALIDKTIAGDVAVIKEVCDLTEGKARHAVVLTYTERE
                     VYELAVREMMDESQCTREEAVRTLGVFRPDALALLDEC"
     gene            complement(1905..2183)
                     /locus_tag="AC159P_14225"
     CDS             complement(1905..2183)
                     /locus_tag="AC159P_14225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004387065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696654.1"
                     /translation="METTTPEVEVTVTAGAPLPPGRHRFQLVVVDDSGNQSEPATAEV
                     IVRDTQRPTAVLDAPREADPGRSFQLSGRRSSDVAPGRIVKYIWTRLD"
     gene            complement(2359..2697)
                     /locus_tag="AC159P_14230"
     CDS             complement(2359..2697)
                     /locus_tag="AC159P_14230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696655.1"
                     /translation="MPIFNGWIIRALAARLRQPGMRPQRRFLAAAGFLMALFVTGAVI
                     YLFRQSTEDAMERAALKVFAAMGVFVALLIGAAVVVRKRTGASWPQEEALRMAGERRG
                     GRRQQRGSRS"
     gene            complement(2867..3253)
                     /locus_tag="AC159P_14235"
     CDS             complement(2867..3253)
                     /locus_tag="AC159P_14235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006835736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HIR3696656.1"
                     /translation="MKKLLAALLVVLSLGVAVGSREPRKKGEKMQFVYVLKLTPGLLE
                     GKGWTEQDSQIIGRHFRRLQQLHQGGTVILAGRTLNESDPSQFGVVIFEAASEEEARK
                     IMEEDDAVKEKVMTAQLFPFKVALGG"
     gene            complement(3407..3952)
                     /locus_tag="AC159P_14240"
     CDS             complement(3407..3952)
                     /locus_tag="AC159P_14240"
                     /inference="COORDINATES: protein motif:HMM:NF024865.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR3696657.1"
                     /translation="MSARHVLFALLSLCVLAGAATAQTMAPAQPAQAASLAAAVVMVS
                     GALTGAQRSAAEQFLELEQGWMDALARKDAAKLEATLAPEFTIVGVGSRADDMVSDRA
                     GWPRLSGQRPWPRHEVKIIKVSKLDRTAVVHCVLTAEYPPRSITPKGGLITFLVTDTW
                     VERGGRWQVLTRHASLPAERD"
BASE COUNT          677 a         1444 c         1460 g          727 t
ORIGIN      
        1 tgctgcgcaa agagggcgag ccactgtggg ttgatctggg cgcagtagag agcgttgacg
       61 gcgatctcgc gcggctgctc gcggcgctga aatgcccgca gacggttgac gacatccgga
      121 agtgtccctc ggtcgccgag gtgaagcggc tggcccgcgc cgccgacgag cgggtgatgc
      181 gcccggtgcg caagctcctc ggcgacacgc ggcacgtctt cgtctcgccg gacggcgcgc
      241 tcaacctcct gcccttcgcc gcgctcgttg acgagaacgg caagtacctc gtcgagacct
      301 actcgctgac ttacctgacg agcggcagag acctgctgcg gctgcaagcc ggtgcgggga
      361 gccgcgagcc gccgctcgtc gtcgccgacc cggcttacga cggtgcgcgc gctttcccga
      421 ctccgaccgc gaggattcag gcgggagagg agtcggcgag gcgttcgggc gagatggggg
      481 cgatgcgatt caggccgctg ccgggcacag ccgcggaggc caaggcgctc ggcgcggcgc
      541 tgccgggcgt gcgggtgctg acacgggagc gggcgaccga ggcggcgctc aagcaggtca
      601 cggcgccgcg cgtgctgcac gtcgccacgc acggcttctt cctccccgac cagccgcagc
      661 agaccgcagc ggaggacgcg cgcgggatca cgctctccgg cggcggcggg cccccagtgc
      721 ccgcacgtgt tgagaacccg ttactgcgct cgggcctcgc gctcgaaggc gccaaccagc
      781 ggcgcggggc gggcggcgag gacggcatcc tgaccgcgct cgaagccgcg gcgctcgacc
      841 tttgggggac gaagctggtg gtgctctcgg cctgcgagac gggcgtcggc gaggtcaaga
      901 acggcgaggg cgtctacggc ttgaggcgcg cgctggtgct ggccgggtcg gagagtctgg
      961 tgatgagcct gtggcaggtc tcggacgagg cgacgaaaga cttgatggtc agctactaca
     1021 agcggctgct ggcgggcggg gggcgcaccg aggctctcag gcaggtgcag ttggaggtgc
     1081 tgaggggcgg cgggcgggcc gcgggcgggc aggggcgctg tctattctgt gagcccggta
     1141 aggcgggcgc gccgcgagac ctgagccacc cctattactg ggcggccttc atccagtcgg
     1201 gcgactggcg cggcatgggc atgcgctagg aaatgaagcg cgccatcagg tcgtccctcg
     1261 tcggctgcgt gccggcgacg tggggggggg acggttcctc actcagcact cgtcgagcag
     1321 cgccagcgcg tcggggcgga agacgccgag cgtcctgacc gcctcctcgc gcgtgcactg
     1381 cgactcgtcc atcatctccc gcacggccag ctcatagacc tcgcgctcgg tgtaggtcag
     1441 cacgactgca tgccgggcct tgccctccgt gaggtcacag acctccttga tgaccgccac
     1501 gtcgcccgcg atggttttgt cgatgagcgc gcgcgcgatg gcgtcggcgt agctctcgac
     1561 gtccgcgccc tccccgccgg gaggggactc ggcgagcacc gcgcgcacgg cgtcggagat
     1621 ggcggcagag cgggggcgcc ccttcgggtt gccgctccgg cccttcttga agcgggtgtg
     1681 gacgggcggc ctgccgtagc cgacttccct gttatttccc tgcacatcag ggattcctgc
     1741 cggtcgtttg tcggccttgt tctaaaaaca ttttcagctt tcctcatacc gagtttaccg
     1801 cggtggatat cgcgccttac ggcgcatagg gcgcgttaat gaaactcgcg gcgcaactac
     1861 ttccggtgca ggccgtcgct aggcgcggct tgcggcccct ccggctaatc cagcctcgtc
     1921 cagatgtact tcacgattct gcccggggcg acatcggacg agcgcctccc cgagagctgg
     1981 aagctgcgcc ccggatccgc ctcccggggc gcgtccagca ccgccgtcgg cctctgcgtg
     2041 tcccgcacga tcacctccgc ggtggccggc tccgattgat tcccgctgtc gtctaccaca
     2101 acgagttgga agcggtggcg gcccggcggg agcggggcgc ccgccgtcac cgtcacctcc
     2161 acctccggcg tggtagtctc cacaggctgc ccgggtctga actccggcat gttttctcct
     2221 cctctttcta tggggacgta ggccgcgcct aaaacccgaa ttgaccgggc ggggcgggcc
     2281 accagaaccg gcaagtcccg cgcatggagg ccgcgcaatt cgcgctccgg ttgagtgagt
     2341 tgtgaggcgg cccgagcatc atgagcggct cccccgttgc tgccgccgcc ctccgcgcct
     2401 ctcgcccgcc atccgcaggg cctcctcttg cggccaactg gcgcccgtcc ttttgcggac
     2461 gacgaccgcg gccccgatga ggagggcgac gaatacgccc atcgccgcga acaccttgag
     2521 cgcggcccgc tccatcgcgt cctccgtgga ctgccggaag aggtagatca cggccccggt
     2581 gacaaacaaa gccatcagga agccggcggc ggccagaaag cgcctctggg gccgcatgcc
     2641 gggttgccgc aggcgcgcgg ccaacgcacg tatgatccag ccgttaaaga tgggcatcac
     2701 agtccccact cgattcgact acaactgtgc cagtccgccc gtccgccgaa cgcccggggt
     2761 cgcacgccgc gacggcccat gctgaacggc aagttctcga tggcatccga cgtgattcgc
     2821 ggtcgggtgc cttccgttgt gaggcgcggc gcagcatagg ccgggttcac ccgccgagtg
     2881 ccaccttgaa ggggaagagt tgcgccgtca tgaccttctc cttcaccgcg tcatcttcct
     2941 ccatgatctt ccgtgcctct tcctccgaag ccgcctcgaa gatcaccacc ccgaattggc
     3001 tcgggtcgga ctcgttcaaa gtcctgccgg ccagaatcac ggtgcctcct tgatgtagct
     3061 gttgcagcct gcggaagtgc ctcccgatga tctggctatc ctgttccgtc caacccttcc
     3121 cttccagcag tccgggggtc agctttagca cgtagacgaa ttgcatcttc tcaccctttt
     3181 tccgcggctc tcgcgagccg accgcaacgc ccagggagag cacgacgagc agggctgcaa
     3241 gaagcttttt catgttcgcc tccgtggatt caattgtgga actagccact ggcaccgaat
     3301 gtccggcatt acccgccgtg cactcaacac cgagaccgca caggctctgg ataaaaggca
     3361 cgcggattcg cggtcgggtg caggccgtcg tcaggcagcg acacggttaa tctctctccg
     3421 cgggcaggct cgcgtggcgt gtgagcactt gccagcgccc accgcgctcg acccacgtgt
     3481 cggttacgag gaacgtaatc aagcccccct taggagtgat actccgcggg gggtactcgg
     3541 cagtgaggac gcaatgcacg accgccgtgc ggtcgagttt gctcaccttg atgatcttca
     3601 cttcgtgccg gggccacggg cgctgcccgg agagcctcgg ccatccggcg cggtcgctca
     3661 ccatgtcgtc cgccctcgat ccgacgccga cgatggtgaa ctccggcgcg agggtggctt
     3721 caagcttcgc ggcatccttc ctagctaggg cgtccatcca cccttgttcc agctcaagga
     3781 attgctccgc ggcggatcgt tgtgcgcctg tcaaggcgcc cgagaccatc accacggccg
     3841 cggcgagcga tgcggcctgc gcgggctggg ccggtgccat cgtctgcgcg gtggcggcgc
     3901 cggccagaac acagagcgag aggagggcga agaggacgtg gcgagcggac atctttgact
     3961 ccttctgcga tcaggggcaa ggtgcaaaga gcgcgagaaa agttgtaggg agcgggccgc
     4021 gtacgcggcg ccgcgcgcta aagataggac ggcgcatgat ggagcgcgag ggcgcgtgcc
     4081 gtcaagacta actggcgggg tcgcggctgg atggtgggcg tgcgctgcac gatcggcaaa
     4141 accacgacga taggtgcgct gacttgtaat agtcagaact tgatctgaca ctcatgacct
     4201 atccgacagc ctgcgcgccg gcatgctccg ccgcgcctcc ggccagcaac atatcaagca
     4261 tcttctcgtc agccaacttc ttcgacgcga ggaaggtctc catcggcg
//
LOCUS       DBNGOH010000736         2114 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3775278, whole genome shotgun sequence.
ACCESSION   DBNGOH010000736 DBNGOH010000000
VERSION     DBNGOH010000736.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2114)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2114)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2114
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3775278"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(87..1937)
                     /locus_tag="AC159P_14245"
     CDS             complement(87..1937)
                     /locus_tag="AC159P_14245"
                     /inference="COORDINATES: protein motif:HMM:NF012688.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HIR3696658.1"
                     /translation="MAEFNYKLVTTHEELSAAAEYLARQPVLGFDTETTALDPYAGRL
                     RLVQLAAPDAVYVFDLFALAPGDGDAARHEALEPLRRLLHAKSPVKVAHNAKFDAKWL
                     RLRLGVELCGEHDSRADGGDGGGAERVERGGLFDTLLASQLISAGEQEDRHSLGSVVA
                     RYLDEEVDKSQQVSDWGGALSAPQLEYAARDAAVLLPLRDKLVEQLRANALGRCAQLE
                     FECVVPVATLELNGLFLDKERWREQLAVVEKKRTQLAAELQDILAEESAQQSFFGPSR
                     VEINLDSHVQLTKALTRLGVPVPDSTRNWKLQPLAHDYPVVAKLLDYRTVQKALTSYG
                     ENILKEISPVTGRVHANFHQIGAPTGRFACTSPNVQQVPHSIEYRRCFRAPEGRKLII
                     SDYSQIELRILADFTGDRGFVDAFNSGADLHRVTAAQVFGVALDAVTKEQRDFAKRLN
                     FGVVYGIGAQRFAMMTGLSQTDAEDILRRYFATYRGLDTWLRDAANKAVRERTAPRTV
                     AGRLFRFNFDPEDRQAASLAQRNGKNSPIQGSSADIIKRALRLLHDRLKGTSACVVNV
                     IHDEVVVETDAEGAEEIARTVEEAMCAAGEEYVKKVPVKVESQIADEWVK"
BASE COUNT          306 a          662 c          782 g          364 t
ORIGIN      
        1 cgccaccgcc gcctctccgc gtcctctgcg ttaaatccga aggttcgaca agctcgaccg
       61 cgaactgttc cgtaccgcgc tcgctctcac ttcacccact cgtccgcgat ctgcgactcg
      121 accttgacgg gcaccttctt gacgtactcc tcgcccgccg cgcacatcgc ctcctcgacc
      181 gtccgcgcga tctcctcggc gccttccgcg tcggtctcga ccacgacctc gtcgtgaatc
      241 acgttgacga cgcacgcgct cgtccccttc aggcggtcgt gcaggagacg cagcgcgcgc
      301 ttgatgatgt cggcgctgct cccttgaatg ggcgagttct tgccgttgcg ctgggcgagc
      361 gaggcggcct gccggtcttc ggggtcgaag ttgaagcgga agaggcggcc cgcgacggtg
      421 cgcggggcgg tgcgctcgcg caccgccttg ttggcggcat cgcggagcca tgtgtcgagg
      481 ccgcggtagg tggcgaagta gcggcgcagg atgtcttcgg catcggtctg cgagaggccg
      541 gtcatcatgg cgaagcgctg cgcgccgatg ccgtagacga cgccgaagtt gaggcgcttg
      601 gcgaagtcgc gctgctcttt cgtcaccgcg tcgagcgcga caccgaagac ctgcgcggcg
      661 gtgacgcggt gcaggtcggc gcccgagttg aaggcgtcca cgaagccacg gtcgccggtg
      721 aagtcggcga ggatgcgcag ctcgatctgc gagtagtcgg agatgatgag cttgcgccct
      781 tcgggcgcgc ggaagcagcg gcggtactcg atggagtgcg gcacctgctg gacgttcggc
      841 gaggtgcacg cgaacctgcc ggtgggcgcg ccgatctggt gaaagttggc gtggacgcgc
      901 ccggtcacgg ggctgatctc tttgaggatg ttctcgccgt agctggtgag cgccttctgg
      961 acggtgcggt agtcgaggag cttggcgacg accggatagt cgtgcgcgag cggttgcagc
     1021 ttccagttgc gcgtggagtc tgggacgggg acgcccaaac gcgtcagagc cttcgtcagt
     1081 tggacgtgcg agtcgaggtt gatctcgacg cgcgaggggc cgaagaagga ctgctgcgcg
     1141 gactcttcgg cgaggatgtc ttgcagctcg gcggcgagct gcgtgcgctt cttctcgacg
     1201 acggcgagct gttcgcgcca gcgctccttg tcgaggaaga ggccgttgag ttcgagcgtg
     1261 gcgacgggga cgacgcactc gaattcgagc tgcgcgcagc gcccgagggc gttggcgcgc
     1321 aactgctcga cgagcttgtc gcgcaaaggg agcagcaccg ccgcgtcgcg cgcggcgtat
     1381 tcgagctgcg gcgccgacag ggcgccgccc cagtcgctca cctgctgcga cttgtcaacc
     1441 tcctcgtcga ggtagcgcgc gacgaccgag ccgaggctgt ggcggtcttc ctgctcgccg
     1501 gcggagatga gctggctggc gagcagcgtg tcgaagagtc cgccgcgctc gacgcgttcg
     1561 gcgccgccgc cgtcgccgcc gtccgcgcgc gagtcgtgct cgccgcacag ctcgacgccg
     1621 aggcgcaggc gcagccactt cgcgtcgaac ttggcgttgt gcgcgacctt gacgggcgac
     1681 ttcgcatgga gcagccggcg cagcggttcg agcgcctcgt ggcgcgccgc gtcgccgtcg
     1741 ccgggcgcga gcgcgaagag gtcgaagacg tagacggcgt cgggcgcggc gagctgcacg
     1801 aggcgcaggc gcccggcgta agggtcgagc gcggtcgtct cggtgtcgaa gccgaggacg
     1861 ggttgccgcg cgaggtactc cgccgccgcg gacagttctt cgtgcgtcgt gaccagcttg
     1921 tagttgaact ctgccatcgg attaatccga tgatgaatgt gctgctgtcg gtgaggtgta
     1981 agctaacacc aaagcggccg gatttcataa cgcgccgcag gagggttgat gaagggaatg
     2041 ctgaccgaaa gtcagtgcgg ccgtgcgtcg cgttagcggc cggcgcgcgc gcgcgcgggc
     2101 gtgggcgagc ggat
//
LOCUS       DBNGOH010000737         6342 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3042687, whole genome shotgun sequence.
ACCESSION   DBNGOH010000737 DBNGOH010000000
VERSION     DBNGOH010000737.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6342)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6342)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6342
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3042687"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2597)
                     /locus_tag="AC159P_14250"
     CDS             complement(<1..2597)
                     /locus_tag="AC159P_14250"
                     /inference="COORDINATES: protein motif:HMM:NF015140.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007154 - cell communication [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Calx-beta domain-containing protein"
                     /protein_id="HIR3696659.1"
                     /translation="MTPNTTRRLAAFAFALSLAIHFAGGTGATAPQPPGALSFSQAAY
                     EVNEGGGSAAITLTRGGGTDGAVTARVGISNVTTNAADYRFTPGALDATFTPGTGPNN
                     VVHDVLVQPDGKILIAGFFRSYAGVTRNNVARLLPSGALDTTFDPQLGPGEVFSMALQ
                     PDGKLVVGGVIFTPDGSSRNGIGRLNADGSVDATFTPGGGTGNHGIDTIALRPDGKIL
                     IGGNFVSYDGVAREGSARLNADGTLDPSFVPPAVFRVFDLLALPDNKVIVCGFFSQIA
                     GVSRPGVARLNEDGSHDLTFVPDAVLNRQSVSTLARQPDGKIIVGGVIFIDQPPSMTS
                     TPLARLNADGSFDPFFFDPGLGRLHFISAITLQPDGKVIVGGAFRTINNPLQDVGQFL
                     HRLNTNGTIDGSFNSGITAQLNSAVEEVALQPDGKLVVGGQFTQPFPGPNVFLRRLQG
                     DLFVTWPDGDAADKTVNLPIVDDLLGESAETLTLTLTPHTGGATTGTHPSATLTIIDN
                     EVAPAITSAPPPGGITRTPYTHTFTATGSPAPTFSVTSGSLPPGLFLNPATGVLSGSP
                     TAAGTFSNITVTAGNGVAPAATQTFSLNILSGGTLQFGTNALSVSEDGASATVTVTRN
                     GGSAGAAAVNFSASNGTASGSGVDFTLVSGTLGFAEGETSKTFAVQITNDDVNERDET
                     LNLFLSSVTGSASLGSPSNAVLTITNDDPLPTISIDDVVVNESDTGVRPAVFTVSRAG
                     LIDRPVSFTASPADGTARANEDYAPFTFTFQIAPNTQSTTVIVNIAGDTITEPDENFV
                     LNISAPINATIVDAQGAGEVKDNESPNTPPTVQFAAWNFNAPEADGAVNINVTRSGDI
                     SAPATVLYTT"
     gene            complement(2937..3446)
                     /locus_tag="AC159P_14255"
     CDS             complement(2937..3446)
                     /locus_tag="AC159P_14255"
                     /inference="COORDINATES: protein motif:HMM:NF014525.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HIR3696660.1"
                     /translation="MKATDIHTGASPAPDINVTPFIDILLVLLIIFMVVTPLRPARFK
                     ALIPEPPPGDAEILLSPRTLVVEVGHDLSLRLIRGRTAVAVGTVADAGGVAARLADEW
                     RERKANGKWKADAFDRADLAPDERIERTVFVRAPRSVRYGEVAKVIDNIKGAGAQPIG
                     LQTDELPHD"
     gene            3694..4851
                     /locus_tag="AC159P_14260"
     CDS             3694..4851
                     /locus_tag="AC159P_14260"
                     /inference="COORDINATES: protein motif:HMM:NF042637.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ARPP-1 family domain-containing protein"
                     /protein_id="HIR3696661.1"
                     /translation="MRTLRGLRAAWGLLCACALASAALLPSASRSAAKAMPHGGGHGG
                     NGYGLWAAQKPVSFKNLTIFPVRGRDAAGAGDYITLDEGIRAGTVVITERGAGTMARR
                     PSRRGDRTQQVNMVAGDGASVNQLALVNRSGKKLLLLSGEVIVGGKQDRIVQEDRVVP
                     PVSVPIALDVFCVEHGRWQQRASATGSMNSAPVVAPASEPRKAENFYSLGAVAHPKLR
                     AASQDKKQQSEVWKEVGENNARLGTANSTDTYQEVYSNKKIAGDMQEYIRALEREVLQ
                     PGVVGVVVARNGELVWADVFASSSLFARYWPKLLKSYAVDAIGDYRNDRVPNVLHAQR
                     YLNEREGTVAAAGQAGVYQLVKIENPHYAVFELRDISLRAPLRLHFNKMNR"
     gene            complement(5052..6188)
                     /locus_tag="AC159P_14265"
     CDS             complement(5052..6188)
                     /locus_tag="AC159P_14265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696662.1"
                     /translation="MAATKKKPTTKKKAAPAKKQKSAAKKKPPQKRAAKKTARATKKR
                     AAKKQATKKGVAKKQATAAKKVAGAAKQKTVVAKKSAAKKSARDVLDLGDVPSALVAR
                     YTTALCLACIFDLFTRRLGLTPRTAYTEIRRHEPTIEELTTREPQRPYFRDEGRNPRC
                     PYCAAAPRWHARLATYRVEGGKTTDAARRALVKQLPARDGEFQFIEKKSTARAAFFEW
                     LERLGQELDFDRDERAWMLAATRRYLGRREPRTDWAEVFDGVRQVRRSHLLAEGWERE
                     GPRLFLAPAVYNDVLLVQYLVSRSHRHGGRTFEGRLTLPELVRRMRFSGHLHAQGIND
                     RDQFDVLEKLVEQLTGGDAPLKLHYIIDRRDLLQKVKDVYARYA"
BASE COUNT          927 a         1979 c         2364 g         1072 t
ORIGIN      
        1 gtcgtgtaga ggacggtggc gggcgcagag atgtcgcccg agcgcgtgac gttgatgttg
       61 acggcgccgt cggcctcggg cgcgttgaag ttccacgcgg cgaactgcac ggtcggcggc
      121 gtgttgggcg actcgttgtc cttcacctcg ccggcgccct gcgcgtctac gatggtggcg
      181 ttgatgggcg ccgagatgtt gaggacgaag ttctcgtccg gctcggtgat ggtgtcgccg
      241 gcgatgttga cgatgacggt cgtgctctgg gtgttgggcg cgatctggaa cgtgaaggtg
      301 aagggggcgt agtcttcgtt ggcgcgcgcc gtgccgtcgg cggggctggc ggtgaagctc
      361 acggggcggt cgatgaggcc ggcgcgcgag acggtgaaga cggcgggcct gacgcccgtg
      421 tcgctctcgt tgacgaccac gtcgtcgatg gagatggtcg ggagcgggtc gtcgttcgtg
      481 atggtcagca cggcgttcga gggcgagccg agcgaggcgc tgcccgtgac ggagctgagg
      541 aagaggttga gggtctcgtc gcgctcgttc acgtcgtcgt tcgtgatctg cacggcgaag
      601 gtcttgctgg tctcgccctc ggcgaagccg agcgtgccgg agacgagcgt gaagtccacg
      661 ccgctgccgc tggccgtgcc gttggaggcg gagaagttga ccgcggcggc gcccgcgctg
      721 ccgccgttgc gcgtgacggt gacggtggcg gacgcgccgt cctcgctcac gctgagcgcg
      781 ttcgtgccga actggagcgt gccgccgctg aggatgttga gggagaaggt ctgcgtggcg
      841 gcgggggcga cgccgttgcc ggccgtgacg gtgatgttgc tgaaggtgcc cgcggcggtg
      901 ggcgagccgg aaaggacgcc cgtcgcgggg ttcaggaaga gtcccggcgg caggctcccg
      961 gaggtgacgc tgaaggtggg cgcgggcgag ccggtcgcgg tgaaggtgtg cgtgtagggc
     1021 gtgcgcgtga tgccgccggg gggcggcgcg ctggtgatgg ccggcgcgac ttcgttgtcg
     1081 atgatggtca gcgtggcgct cgggtgcgtg ccggtggtcg cgccgccggt gtgcggcgtg
     1141 agcgtgagcg tgagcgtctc ggcggactcg ccgaggaggt cgtccacgat gggcaggttg
     1201 acggtcttgt ccgcggcgtc gccgtcgggc caagtgacga agaggtcgcc ttgcagccgc
     1261 cgcaggaaga cgttggggcc ggggaagggc tgcgtgaact gaccgccgac gacgagcttg
     1321 ccgtccggct gcaacgccac ctcttcgacg gcgctgttga gctgcgccgt gatgcccgag
     1381 ttgaaggagc cgtcgatggt gccgttcgtg ttgaggcggt ggaggaactg cccgacatcc
     1441 tgcaaggggt tgttgatcgt cctgaaggcg ccgccgacga tgaccttgcc gtcgggctgg
     1501 agggtgatgg cgctgatgaa gtggaggcgt ccgaggccgg ggtcgaagaa gaacgggtcg
     1561 aagctgccgt cggcgttcag ccgggcgagc ggggtcgagg tcatgctcgg cggctggtcg
     1621 atgaagatga cgccgccgac gatgatcttg ccgtcgggct ggcgggccag cgtgctgacg
     1681 gactggcggt tcaggacggc gtccgggacg aaggtcaggt cgtggctgcc gtcctcgttc
     1741 aggcgcgcga cgccgggccg gctcacgccc gcgatctggc tgaagaagcc gcagacgatg
     1801 accttgttgt cgggcagcgc gagcaggtcg aagacgcgga agacggcggg cgggacgaac
     1861 gaggggtcga gcgtgccatc cgcgttgagg cgcgcgctgc cctcgcgcgc gacgccgtcg
     1921 taggagacga agttgccgcc gatgagaatc ttgccgtcgg gcctgagcgc gatcgtatcg
     1981 atgccgtggt tgccggtgcc gccgcccggc gtgaaggtcg cgtccacgct cccgtccgcg
     2041 ttgagccgcc cgatgccgtt gcgcgaggag ccgtcgggcg tgaagatgac gccgccgacg
     2101 acgagcttgc cgtcgggctg caaggccatc gagaagacct cgccggggcc gagctgcggg
     2161 tcgaacgtcg tgtcgagcgc tcccgaaggc agcagccgcg cgacgttgtt ccgcgtcacc
     2221 cccgcgtacg acctgaagaa accggcgatg aggattttgc cgtcgggctg cacgagcacg
     2281 tcgtgcacga cgttgttcgg ccccgtgccg ggggtgaagg tggcgtccag tgcgcccggc
     2341 gtgaagcggt agtccgccgc gttcgtcgtc acgttcgaga tgccgacgcg cgccgtcacg
     2401 gccccgtcgg tgccgccgcc gcgcgtgagc gtgatggcgg cgctgccacc gccctcgttc
     2461 acctcgtagg ccgcttgcga gaacgagagc gcgccgggcg gctgcggcgc cgtcgcgccc
     2521 gtccctcccg cgaaatggat tgccagcgac agcgcgaagg cgaaggcggc gaggcgccgc
     2581 gtggtgttgg gggtcatctt cttctccggg ggcggcaacg tgaggagtgc tctctctgcg
     2641 caaactcgcg ggcgtcggtc gcgggttaag taataaggtt ccggtcttgc gaagggggcg
     2701 ccgtggcggt ctcgcacggc gcggtgacgg aagtcgggca cgcgccggaa tcggggagac
     2761 gtttcgggcg ctaagggtga cgaaccgcgg gtcgggccga ggcggccacg acgaccggcc
     2821 tcgttcccaa aagggagcga tagtacccgc tcgggcggat ttaatcaaac cgacatcgcc
     2881 ccgccgtcgg gcgcggcgcg ggcggcgcgg gttcagtccc ggcaaaaggc gtgcgttcag
     2941 tcgtgcggga gttcgtccgt ctgcaacccg atgggctgcg cgcccgcacc cttgatgttg
     3001 tcgatgacct tggcgacttc gccgtagcgg acggagcgcg gcgccctgac gaagacggtg
     3061 cgctcgatgc gctcgtcggg cgcgaggtct gcgcggtcga aagcgtctgc cttccatttg
     3121 ccgttggcct tgcgctcgcg ccactcgtcg gcgagccgcg cggcgacgcc gcccgcgtcc
     3181 gccaccgtcc ccacggcgac cgccgtcctg ccgcgaatca gccggaggct caggtcgtgg
     3241 ccgacctcga ccacgagcgt gcgcgggctg agtagaatct ccgcatcgcc gggcggcggc
     3301 tcggggatca gcgctttgaa gcgcgcgggc ctgagcggcg tgacgaccat gaagatgatg
     3361 agcagcacca gcaggatgtc gatgaacggc gtgacgttga tgtccggcgc gggcgacgcg
     3421 ccggtgtgaa tgtccgtggc tttcatctct tcgccaaccc tcctccggtt tcgaccctca
     3481 aggcttagac agcgcggccc gcgaaaagtt cccgcgacga gagacgcacg ccgccggctc
     3541 ttcctgactt cgtccggaca aaaattctcg ggccgctcgc aagaaggccg ccgggccgcc
     3601 gttcttttcg gcagacgacg ccagcgcgcc gcgccctcgc cggaagtgtg gtcgccgttg
     3661 ttacaccggg aaattttttc gaggaggcga gtgatgagaa cactgcgggg attgagggcg
     3721 gcgtggggtc tgttgtgcgc gtgcgcgctc gcgtcggcgg cgctgctgcc ttcggcgtcg
     3781 aggagcgcag cgaaggcgat gccgcacggc ggcggccacg gcggcaacgg ctacggcctg
     3841 tgggccgcgc agaagccggt cagcttcaag aacctgacca tcttccctgt gcgcgggcgc
     3901 gacgccgcgg gcgccgggga ctacatcacg ctcgacgagg gcatcagggc cggcacggtc
     3961 gtcatcaccg agcgcggcgc gggcacgatg gcgcgcagac cgagccggcg cggcgacaga
     4021 acgcagcagg tcaacatggt tgcgggcgac ggcgcttcgg tcaaccagct cgcgctcgtc
     4081 aaccgttcgg gcaagaagct gctcctgctc tcgggcgagg tgatcgtcgg cggcaagcag
     4141 gatcgcatcg tgcaggaaga ccgcgtcgtc ccgcccgtca gcgtgcccat cgcgctcgac
     4201 gtgttctgcg tcgagcacgg tcgctggcag cagcgcgcct cggccacggg cagcatgaac
     4261 tcggcgccgg tcgtcgcgcc tgcgagcgag ccgcgcaagg ccgagaactt ctacagcctc
     4321 ggcgcggtcg cgcacccgaa gctgcgcgcc gcctcgcagg acaagaagca gcagagcgag
     4381 gtctggaagg aggtcggcga gaacaacgcg cggctcggca ccgcgaacag caccgacacc
     4441 tatcaggagg tctactcgaa caagaagatc gcgggcgaca tgcaggagta catccgcgcg
     4501 ctcgaacgcg aggtgttgca gccgggcgtc gtcggcgtcg tcgtcgcgcg caacggcgag
     4561 ctggtctggg ccgacgtgtt cgcctcctcc tcgctcttcg cgcgctactg gcccaagctc
     4621 ctcaagtcct acgccgtgga cgccatcggc gactaccgga acgaccgcgt gccgaacgtc
     4681 ctccacgcgc agcgctacct caacgagcgc gaaggcaccg tcgccgccgc cgggcaggcc
     4741 ggcgtctacc aactcgtcaa gatcgagaac ccgcactacg ccgtcttcga gctgcgcgac
     4801 atctcgcttc gcgcgcccct gcgcctgcac ttcaacaaga tgaacaggtg aagaagtagg
     4861 cagtaggcgg taggcagtag gcagtaggca gttaagacaa aagcgaagag aggcttgaga
     4921 ctggaagtct caagcctctc ctcgcttcat cgcttgtcga attactgctt gccgagcgga
     4981 gaggcttggg actttacctc ccaagcctct tcatctctgt tcttctgctt tgctgcctac
     5041 tgcctacttc ttcacgcgta gcgcgcgtac acgtccttga ccttttggag caggtcgcgg
     5101 cggtcgatga tgtagtggag cttgagcggg gcgtcgccgc cggtgagttg ttcgacgagc
     5161 ttttcgagca cgtcgaactg gtcgcggtcg ttgatgccct gggcgtggag gtggccgctg
     5221 aagcgcatcc ggcggacgag ttcgggcagg gtcaggcggc cctcgaaggt gcgcccgccg
     5281 tggcggtgcg agcggctgac gaggtactgc acgagcagca cgtcgttgta gacggcgggc
     5341 gcgaggaaga ggcgcggccc ctctcgctcc cagccttcgg cgagcaggtg cgagcgccgc
     5401 acctgcctga cgccgtcgaa cacctcggcc cagtcggtgc gcggctcgcg ccgtccgagg
     5461 tagcgccggg tggccgccag catccacgcg cgctcgtcgc ggtcgaagtc cagctcctgc
     5521 ccgagccgtt cgagccactc gaagaaggcg gcgcgggccg tggacttctt ctcgatgaac
     5581 tggaactcgc cgtcgcgcgc gggcaactgc ttgacgaggg cgcggcgcgc ggcgtccgtc
     5641 gtcttgccgc cttcgacgcg gtaggtcgcc agacgcgcgt gccagcgcgg cgcggcggcg
     5701 cagtaggggc agcgcgggtt gcggccctcg tcgcggaagt acgggcgctg cggctcgcgc
     5761 gtcgtcagct cttcgatggt cggctcgtgg cgcctgatct ccgtgtaggc ggtgcgcggc
     5821 gtcaggccga gccggcgcgt gaagaggtcg aagatgcacg cgaggcacag cgccgtcgtg
     5881 tagcgcgcga cgagggccga cggcacgtcg ccgaggtcga gcacgtcgcg cgccgacttc
     5941 ttcgccgccg acttcttcgc gacaaccgtc ttctgcttcg ccgcgcccgc taccttcttc
     6001 gcggcggtcg cctgcttctt cgcgacaccc ttcttcgtcg cctgcttctt cgcggcgcgc
     6061 ttcttcgtgg cgcgcgcggt cttcttcgcg gctctcttct gcggcggctt ctttttcgcg
     6121 gcggacttct gcttcttggc gggggcggcc ttctttttcg tggtgggttt ctttttcgtg
     6181 gctgccatct taatcaacga tcgatgagga ttgaaaagca gttttcggtt ttcggtgttc
     6241 agtcaagtca accgctcttg ctgaagaccg aagaccgaaa gccgaaagcc aggggccata
     6301 agctaaaggc tgaacgccga cgaccgaaaa ccgaaaaccg cc
//
LOCUS       DBNGOH010000738         3600 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1393427, whole genome shotgun sequence.
ACCESSION   DBNGOH010000738 DBNGOH010000000
VERSION     DBNGOH010000738.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3600)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3600)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3600
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1393427"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..939
                     /locus_tag="AC159P_14270"
     CDS             <1..939
                     /locus_tag="AC159P_14270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase type-2 family protein"
                     /protein_id="HIR3696663.1"
                     /translation="SALLTLPAAGLTLEDIGATTESLLGCGATINDINAIRKHTTRLG
                     GGGAARLAAPARTASLIISDVVGSPLDVIASGPTAPDPTTFADAWRVVERHGLQERLP
                     PRVCERLRRGLAGEIEETPKPGDELWSRVHNNIVASNVTAAEAAVVAARERGFEATLL
                     TTSLEGEAGEAGRFAASVARELRGRAAGMRPLLTVLGGETTVTLRGEGAGGRNQELAL
                     AAAEALEGVEGVLLVTLATDGGDGPTDAAGAAVTGETLARGRALGYDADDFLRRNDAY
                     NFFAPLDALLRPGPTLTNVNDLLVIVADAAAPTSRA"
     gene            996..2225
                     /locus_tag="AC159P_14275"
     CDS             996..2225
                     /locus_tag="AC159P_14275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3696664.1"
                     /translation="MDNTAATTTADAGRLRSGQIQIAATAFLALFSIVGIALYGLPYF
                     YDYWFKEFGWTRAQVTSGNTISKLLVGPAFGFLAGWMIDRFGPRRLMLAGVMMAGLAL
                     IGLGNMTALWMFYLFYLFNALGYVFAGPLPNQVLLTRWFDKSRGKAMGFAYLGIGVGG
                     FIVFQLAAFLVGRFGWRYALMALGVLIIAVALPLAYFVKESPDEAAAKTKGAAPTVPI
                     GWAFKSRAFFLLAIGSMCSIGAVGGTNQNLKFYLGPDLHYTDQQIANVGSLVLASSLF
                     GRLLMGWLADRFPKKYVMLLIYLIVAGSIPLLFFASSPTALYLFAVLFGIGLGGDYMI
                     IPLMAAELFGVKVMGRVMGLVLTADGVAEALAPMLAASLRDLTGTYATGFVFLIVLAL
                     TGALAIAMLPRKSQAPS"
     gene            2286..>3600
                     /locus_tag="AC159P_14280"
     CDS             2286..>3600
                     /locus_tag="AC159P_14280"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012523008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HIR3696665.1"
                     /translation="MTNRRDFIKTFVAGGAAALFVPAALAGDTGAALIADASAWPAHP
                     AQDAWAQVPAILKRIKPPTFPQRDFAVTRFGAVADGKTDCTDAFRKAINAAHAAGGGR
                     VVVPAGTFLTGPIHLKSNVNLHVSEGATVKFSQETKKYLPLVFSRWEGMELMNYSPFI
                     YAFEQRNIAVTGAGTLDGQGDHEHWWPWKGQERHGWKRGDPDQKKARKTLEEMTERGT
                     PVPERVFGEGHYLRPQFIQPYRCRNVLIEGVTIRNSPMWEINPVLCQNVTVRKVHIAT
                     HGPNNDGCDPESCKDVLIEDCFFDTGDDCIAIKSGRNADGRRINVPTENIIIRDCVMK
                     EGHGGITVGSEISGGVRNLFAENCRLDSPNLDHALRVKNNAMRGGLLENLYFRNIEVG
                     QVAHAVITIDFNYEEGAKGKHTPVVRNYVVENLRSGKSKHALDVQG"
BASE COUNT          582 a         1240 c         1206 g          572 t
ORIGIN      
        1 tcggcgctgc tgacgctgcc ggcggcgggg ctgacgctcg aagacatcgg cgcgacgacg
       61 gagtccttgc tcggttgcgg cgcgacgatc aacgacatca acgcgattcg taaacacacg
      121 acgcgcctcg gcgggggcgg cgcggcccgc ctcgcagcgc ccgcgcgcac ggcctctttg
      181 attatctcgg acgtggtcgg ctcgccgctc gacgtgatcg cttcggggcc gaccgcgccc
      241 gacccgacga ccttcgcgga cgcgtggcgc gtcgtcgagc gtcacggatt gcaggagcgg
      301 ctgccgccgc gcgtgtgcga gcgtttgcgg agaggactcg cgggcgagat cgaagagacg
      361 ccgaagccgg gcgacgagtt gtggtcgcgc gttcacaaca acatcgtcgc gagcaacgtg
      421 acggcggcgg aggccgcggt cgtcgcggcg cgcgaacggg gcttcgaggc gacgctgttg
      481 acgacatccc tcgaaggcga ggcgggcgag gccgggcgct tcgccgcgtc ggtcgcgcgc
      541 gagctgcgcg ggcgcgccgc gggcatgcga ccgttgctca ccgtgctcgg cggcgagacg
      601 acggtgacgc tccgcggcga aggcgccggt ggccgcaatc aggaactcgc gctcgcggcg
      661 gcggaagcgc tcgaaggggt cgaaggcgtg ctgctcgtta cgctggcgac tgacggcggc
      721 gacgggccga cggacgcggc gggcgcggcg gtcacgggcg agacgctggc gcgcgggcgc
      781 gctctcggct atgatgcgga cgacttcctc cgacgcaacg acgcatacaa ctttttcgcg
      841 ccgctcgacg cgctcctgcg acccggcccg acgctgacca acgtcaacga cctgttggtc
      901 atcgtcgccg acgccgcggc ccccacgtcg agggcataag agagaacccg tcccgactcc
      961 cgaactttct gcgaagcgcc gaagagaaaa cctgcatgga caacacagcc gcaacgacaa
     1021 cagccgacgc cgggcgcctg cgctccggcc agattcagat tgccgcgacc gccttcctcg
     1081 cgctcttctc catcgtcggc atcgcgctct acgggctgcc gtacttctac gactactggt
     1141 tcaaggagtt cggctggacg cgcgcgcaag tcacttccgg caacacgatc agcaagctcc
     1201 tcgtcgggcc tgccttcggc ttcctcgcgg gctggatgat cgaccgcttc ggcccgcggc
     1261 ggctgatgct ggcgggcgtc atgatggcgg gcctcgcgct gatcggcctc ggcaacatga
     1321 cggcgctgtg gatgttctac ctcttctacc tcttcaacgc gctcggctac gtcttcgcgg
     1381 ggccgctgcc gaatcaggtg ctcctgacgc gctggttcga caagtcgcgc ggcaaggcga
     1441 tgggcttcgc ctacctcggc atcggcgtcg gcggcttcat cgtcttccag ctcgcggcgt
     1501 ttctggtcgg gcgcttcggc tggcgctacg cgctgatggc gctcggcgtg ctcatcatcg
     1561 ccgtcgcgct gccgctggcc tacttcgtca aggagtcgcc ggacgaggcg gcggcgaaga
     1621 cgaagggcgc ggccccgacc gtgcccatcg gctgggcgtt caagagccgc gcgttcttcc
     1681 tgctcgccat cggcagcatg tgctccatcg gcgcggtcgg cgggacgaac cagaacctca
     1741 agttctacct cgggccggac ctccactaca ccgaccagca gatcgccaac gtcggctcgc
     1801 tcgtcttggc gtcgagcctc ttcgggcggc tgctgatggg ctggctcgcc gaccgcttcc
     1861 cgaagaagta cgtgatgctt ttgatctacc tcatcgtcgc cggctcgatc ccgctcctct
     1921 tcttcgcctc ctcgccgacg gcgctctacc tcttcgccgt cctcttcggc atcggcctcg
     1981 gcggcgacta catgatcatc ccgctgatgg ccgccgagct gttcggcgtc aaggtgatgg
     2041 ggcgcgtgat ggggctggtc ctgaccgccg acggcgtggc cgaggcgctc gccccgatgc
     2101 tcgcggcgtc gctgcgcgac ttgaccggca cctacgccac gggcttcgtc ttcctcatcg
     2161 tgctcgcgct gacgggcgcc ctcgccatcg cgatgctgcc gcggaagagt caggcgccga
     2221 gctaggcgct gttcggaatc ttcggcgcgc gtgacggatt cacgttttcg aatcgagagg
     2281 gaagcatgac caaccgacgc gacttcatca agaccttcgt ggccggcggc gcggcggcgc
     2341 tcttcgtgcc cgccgcgctc gccggcgaca ccggcgcggc gttgatcgcc gacgcgtcgg
     2401 cgtggcccgc gcatccagcg caagacgcgt gggcgcaggt gcccgcgatt ctgaaacgca
     2461 tcaaaccgcc cacgttcccg cagcgcgact tcgccgtgac gcgcttcggc gccgtcgcgg
     2521 acggcaagac cgattgcacc gacgcgttcc gcaaggcgat taacgcggcg cacgcggcgg
     2581 gcggcgggcg cgtggtcgtg cccgcgggca cgttcctcac cggcccgatt catctgaagt
     2641 cgaacgtcaa cctccacgtc tccgaaggcg cgaccgtcaa gttcagtcag gagacgaaga
     2701 agtatctgcc gctcgtcttc tcgcgctggg aggggatgga actgatgaac tactcgccgt
     2761 tcatctacgc cttcgagcag cggaacatcg ccgtcacggg cgcgggcact ttggacgggc
     2821 agggcgacca cgagcactgg tggccgtgga aggggcagga gcgccacggc tggaagcgcg
     2881 gcgaccccga ccagaagaag gcgcgcaaga cgctcgaaga gatgaccgag cgcggcacgc
     2941 ccgtgccaga gcgcgtcttc ggcgaaggcc attacctgag gccgcagttc atccagccct
     3001 accgctgccg gaacgtcctc atcgaaggcg tcaccatcag gaactcgccg atgtgggaga
     3061 tcaaccccgt gctctgccag aacgtcacgg tgcgcaaagt ccacatcgcc acgcacgggc
     3121 cgaacaacga cggctgcgac cccgagtcgt gcaaggacgt tttgatcgag gactgcttct
     3181 tcgacaccgg cgacgactgc atcgccatta agtcggggcg caacgccgac ggtcggcgca
     3241 tcaatgtgcc gaccgagaac atcatcatcc gcgactgcgt gatgaaggaa gggcacggcg
     3301 gcatcaccgt ggggagcgaa atctcgggcg gcgtgcgcaa cctcttcgcg gagaactgcc
     3361 gcctcgacag ccccaacctc gaccacgcgc tgcgcgtcaa gaacaacgcc atgcgcggcg
     3421 gcctgctgga aaatctttac ttccgcaaca tcgaagtcgg gcaggtggcg cacgccgtca
     3481 tcaccatcga cttcaactac gaggagggcg ccaagggcaa gcacacgccc gtcgtccgca
     3541 actacgtcgt cgagaacctg cgcagcggca agagcaagca cgcgctcgac gtgcagggct
//
LOCUS       DBNGOH010000739         3976 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4324808, whole genome shotgun sequence.
ACCESSION   DBNGOH010000739 DBNGOH010000000
VERSION     DBNGOH010000739.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3976)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3976)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3976
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4324808"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1341)
                     /locus_tag="AC159P_14285"
     CDS             complement(<1..1341)
                     /locus_tag="AC159P_14285"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HIR3696666.1"
                     /translation="MKECPVCFRCFPDQVNHCPGDGESLKNSLQGDTVLDGRYQLEKR
                     LGQGGMGIVFKARHIFLKTQHAIKVILPDLVGNDPSLATRFRQEAMAAAAIQHPNTVS
                     VTDYGIINGTMPFLVMEFVEGRSLHDVLQIEGKLPPAIALEIMLAICEGVGAAHREEI
                     VHRDLKPLNILLKVGHAPSAGVKVLDFGLAKIKSGELLGSFVAAQTQGMMGSPYYMAP
                     EQWGDEEIDRRADIYSLGVILYQMLTGEVPFKGNSVPSIMNKHLTTPPLPLHDRGVFV
                     PPEVERAVRHALGKGVEARPQTIEEFIAELREAAAAVKASEAAAAQPEVGMNTVEIPP
                     GDTMGGASVDRATALGGEQPGGVGAGPGTVADAQVAARTGVVEEPGVFGEEAPPPDGD
                     AVVAVKPTTPLSDSMLPPDGKSARGAETAHQRAGATGYHPGEADDRGVKTSHGEA"
     gene            complement(1556..2266)
                     /locus_tag="AC159P_14290"
     CDS             complement(1556..2266)
                     /locus_tag="AC159P_14290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696667.1"
                     /translation="MTSSKCMGCGLVNFAAAGACKRCGAALFGGGGGVKAFGRDGAVD
                     EAGGEPRRGLLRKVLAGFVLSFFALVGCYVSLLETSEAANLDQRLLVGRSVDIIERGG
                     FGREAFLLRRLASYRTTDHWWNRWLGHAEAYAATNFPFEVVTLYPEFFQYPTDDVERA
                     VVLLHEARHLAGQGEEEAFASVWRDKARLGWTKERYGHTRVWRNVREFTARNAPEMFQ
                     CGADRRQDCTEEVMRTED"
     gene            complement(2502..3647)
                     /locus_tag="AC159P_14295"
     CDS             complement(2502..3647)
                     /locus_tag="AC159P_14295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005034251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HIR3696668.1"
                     /translation="MPAKIDDFLRAASQLGASDLHIKAGSQPFIRISGELRPLPDTPR
                     LTQEDTLDMAFSMMSNRQKQRFKEVSEADIAYGVSGLGRFRANIFQQRGTVGAVLRVI
                     PDHVRSIRDLGLPPVIEKIAEEQRGLVLVTGTTGSGKSTTLASIIDLINATRCGHLVT
                     IEDPIEFLHRDKKCFVTQREVDVDTRSFAEALRGALRQDPDVILVGEMRDFDTIETAL
                     VAAETGHLVLSTLHTLDATETIRRIVSAFPPHLQKSVRIQLAGILRAVVSMRLVRSAH
                     GLGRVPACEVMVTTGLIRDYIINEEKTSLIRDAIAQGTSQYGMQTFDQSLFSLYQTGR
                     ITIEEALRGASNADEFRLRVAGVRSSSDQAREEMERVISVTSAQRRK"
BASE COUNT          611 a         1289 c         1400 g          676 t
ORIGIN      
        1 cgcctcgccg tggctggtct tcacgccgcg gtcgtccgcc tcgccggggt gatagcccgt
       61 cgcccccgcg cgctggtgcg ccgtctccgc gccgcgcgcc gacttgccgt ccggcggcag
      121 catggagtcc gacaagggcg tcgtgggctt caccgccacg accgcgtcgc cgtccggcgg
      181 cggcgcttcc tctccgaaga cgcccggctc ttcgaccacg cctgtgcgcg cggcgacctg
      241 cgcgtcggcg accgtgccgg ggcctgcgcc gacgccgccc ggctgctcgc cgccgagcgc
      301 cgtcgcgcgg tctacactcg cgccgcccat cgtgtcgccg ggcgggattt ccaccgtgtt
      361 catcccgact tcgggctgcg ccgcggcggc ctccgaagcc ttgacggcgg cggcggcctc
      421 gcgcaactcc gcgatgaact cttcgatcgt ctgcggtcgg gcctcgactc ccttgccgag
      481 cgcgtggcgc acggcccttt ccacctccgg cgggacgaag acgccgcggt cgtgcagcgg
      541 caggggcggc gtcgtcaggt gcttgttcat gatcgagggg acggagttcc ccttgaacgg
      601 cacttcgccc gtcagcatct ggtagaggat gacgcccaga ctgtagatgt ccgcgcggcg
      661 gtcgatctct tcgtcgcccc actgctcggg cgccatgtag tagggcgacc ccatcatgcc
      721 ttgcgtctgc gccgcgacga acgagccgag cagctcgccc gacttgatct tcgccagccc
      781 gaagtccaac accttcacgc ccgcgctcgg cgcgtggccc accttcaaga ggatgttcag
      841 gggcttcagg tcgcggtgga cgatctcctc gcggtgcgcg gcgccgacgc cctcgcagat
      901 ggcgagcatg atttcgagcg cgatcgcggg cgggagcttg ccctcgattt gcagcacgtc
      961 atggagcgag cgcccctcga cgaactccat cacgaggaag ggcatcgtcc cgttgatgat
     1021 gccgtagtcg gtgacgctga cggtgttcgg gtgctggatg gcggcggcgg ccatcgcctc
     1081 ctgacggaag cgcgtggcga gcgaggggtc gttgccgacc aggtcgggga ggatgacttt
     1141 gatcgcgtgc tgcgtcttga ggaagatgtg gcgggccttg aagacaatgc ccatcccgcc
     1201 ctgtccgagc cgcttctcga gttgataacg accgtcgagc acggtgtcgc cctgaaggct
     1261 gttcttcaac gactcgccgt cgcccggaca gtggttgacc tgatcgggga agcagcggaa
     1321 gcagactggg cactctttca tctgaaggct ctacgaagca ccgaccagca agagcggacg
     1381 acggatgtcg ataaaggggc gcggcacacg ccgacgaagt aacatgcgaa aaattctaac
     1441 aactgaacgg gaagcaggca accataagga ctgaggactg agtaagcagc gacgggtgac
     1501 ggatgattag aggcacgctg tttctcatca tccgtcaccc gtggcttctt attactcagt
     1561 cctcagtcct catcacttct tccgtgcagt cctgccgccg gtcggcgccg cactggaaca
     1621 tctctggggc gttgcgcgcg gtgaactcgc ggacgttgcg ccagacgcgg gtgtggccgt
     1681 agcgctcctt cgtccagccg agccgcgcct tgtcgcgcca caccgaggcg aaggcttcct
     1741 cctcgccctg tcccgcgagg tggcgggcct cgtgcaggag gacgacggcg cgctccacgt
     1801 cgtcggtcgg gtactggaag aattccgggt agagcgtgac gacctcgaac gggaagttgg
     1861 tcgcggcgta cgcctcggcg tggccgagcc agcggttcca ccagtggtcg gtcgtgcggt
     1921 agctggcgag gcggcgcagc aggaaagcct cgcgcccgaa gcccccgcgc tcgatgatgt
     1981 ccacggagcg cccgacgagc aggcgctgat ccaggttcgc cgcctcggaa gtctcaagca
     2041 gagagacgta gcagcccacg agcgcgaaga acgacagcac gaagcccgcc agaaccttcc
     2101 tcaacagccc gcgccgcggc tccccgcccg cctcgtccac ggccccgtca cgcccgaacg
     2161 ccttcacccc gccgccaccg ccgaacagcg ccgcgccgca gcgcttgcac gcgcccgccg
     2221 cggcgaagtt gaccagcccg cagcccatgc acttcgaact tgtcattcga tgctgaagac
     2281 tcgtgagtga ttcgccgcgt agcggcgggg tgagtttagc ccggcctttc aaggccgggg
     2341 caggcgggac gtacgttacc ggcgtcgcgt cagcgacgct tgaaccttct ctcagtcgtc
     2401 gctgacgcga cgagggctgc gttgccgcat ctcacccggc gttgaaacgc cgggctacat
     2461 tcatgacgcc gctacgcggc ggcgcggcgc acgcgatgaa cctactttct gcgctgcgcg
     2521 ctggtgacgg agatgacgcg ctccatctct tcgcgggctt ggtcggagga ggagcgcacg
     2581 cccgccacgc ggaggcggaa ctcgtcggcg ttcgaggcgc cgcgcagcgc ctcttcgatg
     2641 gtgatgcgcc cggtctggta gaggctgaag agcgactggt cgaaggtctg catgccgtac
     2701 tgcgaggtgc cctgcgcgat ggcgtcgcgg atgagcgagg tcttctcctc gttgatgatg
     2761 tagtcgcgga tgaggccggt cgtgaccatc acctcgcagg cggggacgcg gcccagcccg
     2821 tgtgcggagc gcacgaggcg catcgagacg acggcacgca ggatgccggc gagctggatg
     2881 cggacgctct tttgcaggtg cggcgggaag gccgagacga tgcggcggat ggtctcggtg
     2941 gcgtcgagcg tgtgcagcgt cgagaggacg aggtggccgg tctcggcggc gacgagcgcc
     3001 gtctcgatgg tgtcgaagtc gcgcatctcg ccgacgagga tcacgtccgg gtcttgccgc
     3061 agggcgccgc gcagggcttc ggcgaacgag cgcgtgtcca cgtccacctc gcgctgcgtg
     3121 acgaagcact tcttgtcgcg gtggagaaat tcaatcgggt cttcgatggt gacgaggtgg
     3181 ccgcagcgcg tcgcgttgat gaggtcgatg atggaggcga gcgtggtgga cttgccggag
     3241 ccggtcgtgc ccgtgacgag gacgagtccg cgctgctcct cggcgatctt ctcgatgacg
     3301 ggcggcagcc cgaggtcgcg gatcgagcgc acgtggtcgg ggatgacgcg caggactgcg
     3361 ccgacggtgc cgcgctgctg gaagatgttg gcgcggaagc gcccgaggcc cgagacgccg
     3421 taggcgatgt cggcctccga gacctctttg aagcgctgct tctgccggtt cgacatcatg
     3481 gagaaggcca tgtcgagcgt gtcttcctgc gtcaggcgcg gcgtgtcggg caggggccgc
     3541 agctcgccgc tgatgcggat gaagggctgc gagccggcct tgatgtgcaa gtcggaggcg
     3601 ccgagctgcg aggccgcgcg caggaagtcg tcaatcttgg caggcatgcg tgtgtttcga
     3661 gacctctctt cggggaaggc cggacgtgtg tgcgagtgtc tgtatcgagg agtcgggcgc
     3721 gctctatata aagagtgctt tcaaaaccgg gatggctcgc agggctttgt ctggcgggat
     3781 gtgctgccgg aaagttgaag gcccggcgag caccgaatac attctagcgt ggccgcttcc
     3841 gcgcggtcaa ccccgcggtt tttcaggggg atgcgcaagg ccgcgggtct gtccggcgtc
     3901 gcccctctgc cggtcgggct tcagtcgggg aggccgaccc gccgcacgag accctgaaag
     3961 cgccgatccg agcgca
//
LOCUS       DBNGOH010000740         2627 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3960423, whole genome shotgun sequence.
ACCESSION   DBNGOH010000740 DBNGOH010000000
VERSION     DBNGOH010000740.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2627)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2627)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2627
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3960423"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..377)
                     /locus_tag="AC159P_14300"
     CDS             complement(<1..377)
                     /locus_tag="AC159P_14300"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF015000.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HIR3696669.1"
                     /translation="MTRDARTFSSTTPLIIGHRGASALAPENNLDAFERALDDGADGV
                     EFDVRLARDRVPVVIHDATLRRTLLRDETVAALTSSELRRWDAGTWFTLRFPERARDD
                     YHLARVPTLDETLGLVGPRSAAV"
     gene            complement(374..1981)
                     /locus_tag="AC159P_14305"
     CDS             complement(374..1981)
                     /locus_tag="AC159P_14305"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012033378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
                     /protein_id="HIR3696670.1"
                     /translation="MTGYFSLILHAHLPFVRHPEHPEFLEEDWLFEAITEVYLPLVAL
                     LRKLCDERIAPRLALTVSPTLAEMLADSLLRTRYERHLENLLALATKELARTRRDAPH
                     FHATALLYHDRLTAALRLWRDTLKRDLVAAFRDLQTAGVVELITCCATHGFLPLISTE
                     EARRAQVLAALANHEKHFGHRPRGIWLPECAYAPGLEKYLAGTGLEYFVADTHAVLYG
                     EPRPRYGAHSPVLTPSGVAVFARDPETSRQVWSAEGGYPGDPFYREFYRDLGFDAPHE
                     YLQPHLHRDGARRHLGLKYHRITGRDVPLQDKQPYDPARACERAAEHARHFVHERLKQ
                     VRRLRETFGDGRAPLVVAPYDAELFGHWWYEGLQFLELVFRELRTPGLRGDIEPITPG
                     DFLDLGLPLQTQQPAQSSWGAGGYFAVWLNEKTSWMYPHQHAAEARMNALARRFRRAP
                     DPLTRRALAQAARELLLLQSSDWAFQIAHDTTAAYAARRFRTHLRRFHALADDLDRGH
                     IDEQRLAEMETRDRLFAELDYTIYGST"
     gene            complement(2072..>2627)
                     /locus_tag="AC159P_14310"
     CDS             complement(2072..>2627)
                     /locus_tag="AC159P_14310"
                     /inference="COORDINATES: protein motif:HMM:NF027583.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4912 domain-containing protein"
                     /protein_id="HIR3696671.1"
                     /translation="LRDHEPDTSADELPETVGRDSVQLFAQSPHKLFVHWGHAGDPFA
                     ALREAFGDAASRYRLVVRLSCMTDDSAQTDDASPERAQWLAALPDRDYRADIGFHADG
                     LPFITLLSSSVVHTPRAAVSPNADAEPQFHIAPDDFSRLLEHTGYDPGTLEQPATTQD
                     ARDPKPFAYCHFSSGQWSVKDSSL"
BASE COUNT          389 a          685 c         1112 g          441 t
ORIGIN      
        1 tagacggctg cggagcgcgg gccgacaagc ccaagcgttt cgtcgagcgt ggggacgcgc
       61 gcgaggtggt agtcgtcgcg ggcgcgttcg gggaagcgga gggtgaacca agtgcccgcg
      121 tcccaccgtc gaagttcgga ggaagtgagg gcggcgaccg tttcgtcgcg cagaagggtg
      181 cggcggagcg tggcgtcgtg gatgacgacg gggacgcggt cgcgcgcgag acgcacgtcg
      241 aactcgacgc cgtcggcgcc gtcgtcgagg gcgcgctcga aggcgtctag gttgttctcg
      301 ggcgcgagcg cggaggcccc gcggtggccg atgatgagag gcgtggtgct tgaaaatgtc
      361 cgtgcgtcgc gggtcatgtg gaaccgtaga tagtgtagtc gagttcggca aagagacggt
      421 cgcgggtttc catttcggcg agacgttgtt cgtcgatgtg tccgcggtcg aggtcgtcgg
      481 cgagcgcgtg gaagcggcgg aggtgcgtgc ggaagcgtcg ggcggcgtag gcggcggtgg
      541 tgtcgtgcgc gatttggaaa gcccagtcgc tcgattgcag gaggaggagt tcgcgtgcgg
      601 cctgcgcgag ggcgcggcgc gtgagcgggt cgggcgcgcg gcggaagcgt cgggcgaggg
      661 cgttcatgcg cgcttcggcg gcgtgctggt gcgggtacat ccaggaagtc ttttcgttga
      721 gccagacggc gaagtagccg cccgcgcccc aggaggattg ggcgggttgc tgcgtttgca
      781 gcgggaggcc gaggtcgagg aagtcgccgg gcgtgatggg ttcgatgtca ccgcgcagac
      841 cgggcgtccg tagctcgcgg aagacgagtt cgagaaactg taagccctcg taccaccagt
      901 ggccgaagag ttccgcgtcg tagggggcga cgacgagcgg ggcgcgaccg tcgccgaagg
      961 tctcgcgcaa gcgacggact tgcttgaggc gttcgtggac gaagtggcgc gcgtgttcgg
     1021 cggcgcgctc gcacgcgcgg gcggggtcgt agggctgctt gtcttggagc ggcacgtcgc
     1081 gtccggtgat gcggtggtac ttcaggccga ggtgtcggcg cgcgccgtcg cggtgcaggt
     1141 gcggctggag gtattcgtgc ggcgcgtcga agccgaggtc gcggtagaac tcgcggtaga
     1201 aggggtcgcc ggggtagccg ccttcggcgc tccagacttg ccggctggtt tctgggtcgc
     1261 gcgcgaagac ggcgacgccc gacggggtga gcacgggcga gtgggcgccg tagcgcggtc
     1321 gcggctcgcc gtagaggacg gcgtgcgtgt cggcgacgaa gtattcgagg cccgtgccgg
     1381 cgagatactt ttcgaggccg ggcgcgtagg cgcactcggg cagccagatg ccgcgcggtc
     1441 tgtggccgaa gtgcttctcg tggttggcga gggcggcgag gacttgcgcg cgccgcgcct
     1501 cttcggtcga gatgagcggg aggaagccgt gcgtggcgca gcaggtgatg agttcgacga
     1561 cgcccgcggt ctgcaagtcg cggaaggcgg cgacgaggtc gcgcttcagc gtgtcgcgcc
     1621 agaggcggag ggccgcggtc agtcggtcgt ggtagaggag ggccgtcgcg tgaaagtgcg
     1681 gcgcgtcgcg gcgtgtgcgc gcgagttctt tcgtggcgag cgcgagaaga ttttcaaggt
     1741 ggcgttcgta gcgcgtgcga agaagcgaat cggcgagcat ctcggcgagc gtgggcgaga
     1801 cggtcagggc gaggcgcggc gcgatgcgct cgtcgcatag cttgcgaaga agggcgacga
     1861 gcggcaggta gacttcggtg atcgcttcga agagccaatc ctcttcgagg aattcggggt
     1921 gctcggggtg gcgcacgaag gggagatgcg cgtggaggat aaggctgaag tagccggtca
     1981 aggcagtggt cggtggtcag tggtcagtgg gttgcgttcg gtggtcggcc attggtcgtc
     2041 ttacagcgtt ctgtcggcat cggcttgatg attaaagtga actgtctttc actgaccact
     2101 gcccactgct gaagtggcag taggcgaaag gcttcggatc gcgcgcgtcc tgtgtggtcg
     2161 ctggctgttc gagggtgcca gggtcgtagc ctgtgtgttc gaggaggcgc gagaagtcgt
     2221 ccggggcgat gtggaattgc ggctccgcgt cggcattcgg cgagacggcg gcgcggggcg
     2281 tgtggacgac cgatgaagag agaagagtga tgaaggggag gccgtcggcg tggaagccga
     2341 tgtcggcgcg gtagtcgcgg tcgggcagcg cggcgagcca ttgcgcgcgc tcgggcgagg
     2401 cgtcgtcggt ttgcgcggag tcgtccgtca tgcaggagag ccggacgacg aggcggtagc
     2461 gcgaggcggc gtcgccgaac gcctcgcgca gcgccgcgaa agggtcgcca gcgtggcccc
     2521 aatggacgaa gagcttgtgg ggcgactgcg cgaagagctg aaccgagtcg cgcccgacgg
     2581 tctcgggcag ctcgtctgcc gatgtgtccg gctcgtggtc ccgcagg
//
LOCUS       DBNGOH010000741         2333 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2309871, whole genome shotgun sequence.
ACCESSION   DBNGOH010000741 DBNGOH010000000
VERSION     DBNGOH010000741.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2333)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2333)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2333
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2309871"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2244
                     /locus_tag="AC159P_14315"
     CDS             <1..2244
                     /locus_tag="AC159P_14315"
                     /inference="COORDINATES: protein motif:HMM:NF012493.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR3696672.1"
                     /translation="SAEQIGRPQIVALTATATPYVRADIIEQLGLREPRAFVSGFDRP
                     NLSLYVVHTQKEREKIAHVRSLAEEEYPGQSGIIYASTRKAVEQVAQRLREAKLNAVG
                     YHAGMADEERARAQDDFMAGRAQMIVATNAFGMGIDKPDIRFVTHYQMPGSVEAYYQE
                     IGRAGRDGEPSSCVLLFNYADKRTQDYFIEGSYPPPELIAKVYEALISTGQKRIELST
                     REIAARASVRNEMAVQSALITLEKAGHIERGAAGENRAGIRLLMPPQTAREAVGARRS
                     AQMKQVLFGLLGGYEINQRADVELDLNDLAESLGMELQSVRRTLGSLVEAGVVAYQPA
                     RRTRGLIMLDEQPVSRLRIRPEDLARRAALEQRKLREVISFCYTDDCFRAFILDYFGD
                     RGHPKRCGTCGNCLLRERRERAVAGPDAFDAPAEFGDAPQDFGDAPPPEPQSALDRFV
                     MRHVPVALDLEEELEGQSRRRRQREAAERVRLDETDLGEINVGSVAVNEPRALSEDES
                     LRVRKILACAARMGGRFGKGLLAATLRGSRSAKIAQSGLDQLSTYGLLADMTQDEISV
                     YVDALVAAGCLGVTGGAYPTVTLTPLGGEVMRERASVELPLPPPYVPAPFAPRSSGSS
                     SSRPAAPSSTAAGQPAAPRPSTTEETYALYEQGLTVEEICEARGLKEITVEKHLADCI
                     LAGRPFDVSRHVSEDARVLIERAVEQLGPERLKPLREALPRHITYRMIRFVVADLQRA
                     SAEGGKG"
BASE COUNT          396 a          852 c          749 g          336 t
ORIGIN      
        1 tcggccgaac agatcggccg cccgcagatc gtcgcgctca ccgcgacggc gacgccctac
       61 gtgcgcgccg acatcatcga gcagttgggc ctgcgcgagc cgcgcgcctt cgtctccggc
      121 ttcgaccgcc cgaacctctc gctctacgtc gtccacacgc agaaggagcg cgagaagatc
      181 gcgcacgtcc gctcgctcgc cgaggaagag tatccgggcc agtccggcat catctacgcc
      241 tcgacgcgca aggcggtcga gcaggtcgca caaaggttac gggaagcgaa gctgaacgcc
      301 gtcggctacc acgcggggat ggccgacgag gagcgagcgc gcgcgcagga cgacttcatg
      361 gcgggccgcg cgcagatgat cgtcgccacc aatgccttcg gcatgggcat cgacaagccc
      421 gacatccgct tcgtcacgca ctaccagatg cccggctccg tcgaggctta ctatcaggag
      481 atcggtcgcg cgggccgcga cggcgaacct tcgtcgtgcg tgctcctctt caactacgcc
      541 gacaagcgca cgcaggacta cttcatcgaa ggctcttacc cgccgcccga actgatcgcc
      601 aaagtctacg aggctttgat ttcgaccggc cagaaacgca tcgagctttc gacgcgcgag
      661 atcgccgcgc gcgcctctgt ccgcaacgag atggcggtgc agtccgcgct catcactctg
      721 gagaaggcgg gccacatcga gcgcggcgcg gcgggcgaga accgcgcggg aatcagactg
      781 ttgatgccgc cgcagacggc gcgcgaggcg gtcggcgcgc gccgcagcgc gcagatgaag
      841 caagtgctgt tcggactcct cgggggctac gaaatcaacc agcgcgccga tgtcgaactc
      901 gatttgaacg acctcgccga atcgctcggc atggagcttc agtccgtcag gcgtactctc
      961 ggttcgctcg tcgaggcggg cgttgtcgcg tatcaaccgg cgcggcgcac ccgcggcctc
     1021 ataatgctcg acgagcagcc ggtcagccgt ctccgcatcc ggcccgaaga cctcgcgcgc
     1081 cgcgccgcgc tcgaacagcg caagctgcgc gaggtcatct ccttctgtta caccgacgac
     1141 tgcttccgcg ccttcatcct cgactacttc ggcgaccgcg gccaccccaa acgctgcggc
     1201 acctgcggca actgcctgct gcgcgagcgg cgcgagcgcg ccgtcgccgg gccggacgcg
     1261 ttcgacgccc cggctgagtt cggcgacgcc ccgcaggact tcggcgacgc gcccccgccc
     1321 gaaccgcaga gcgcgctcga ccgcttcgtg atgcggcacg tgcccgtcgc gctcgacctc
     1381 gaagaagagc tggaaggcca gtcgcgtcgc cgccgccagc gcgaggcggc ggagagggtg
     1441 cggctcgacg agacagacct cggcgagatc aacgtcggct cggtcgccgt caacgagccg
     1501 cgcgcgctct cggaggacga gtcgctgcgc gtgcgcaaga ttctcgcgtg cgccgcgcgc
     1561 atgggcgggc gcttcggcaa ggggctgctc gcggccaccc tgcgcggctc gcgctcggcg
     1621 aagatcgcgc agtcaggact cgaccagctc tcgacctacg gcctgctcgc agacatgacg
     1681 caggacgaaa tctccgtgta cgtggacgcg ctcgtcgccg ccggctgcct cggcgtcacc
     1741 ggcggcgctt atccgaccgt cacgctcacc ccgctcggcg gcgaggtgat gcgcgagcgc
     1801 gcgagcgtcg agctgccgct cccgccgccg tacgtgcccg cgcccttcgc gccgcgcagc
     1861 agcggcagta gttcgtcccg tccagcggcg ccttcatcga ccgccgcggg gcaaccggcg
     1921 gcgccgcgcc cgagcacgac cgaagagacc tacgcgctct acgaacaggg cctcaccgtc
     1981 gaagagattt gcgaggcgcg cggcctgaag gagatcacgg tcgagaagca cctcgccgac
     2041 tgcatcctcg cgggtcgccc cttcgacgta tcgcgccacg tctccgaaga cgcgcgcgtc
     2101 ttgatcgaac gcgccgtcga acagctcggc cccgaacgcc tcaagcccct gcgcgaggcg
     2161 ctcccgcgcc acattaccta ccgcatgatc cgcttcgtcg tcgccgacct ccaacgcgca
     2221 agcgcagaag ggggaaaagg ttaaaagggg aaaaggcgaa gagggaaagg cacaacactt
     2281 ttccccgcct ccccttttcc ccttataacc ttttcgcctg cgtcacgctt cgg
//
LOCUS       DBNGOH010000742         1859 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3227291, whole genome shotgun sequence.
ACCESSION   DBNGOH010000742 DBNGOH010000000
VERSION     DBNGOH010000742.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1859)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1859)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1859
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3227291"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..865)
                     /gene="hslO"
                     /locus_tag="AC159P_14320"
     CDS             complement(<1..865)
                     /gene="hslO"
                     /locus_tag="AC159P_14320"
                     /inference="COORDINATES: protein motif:HMM:NF001033.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HIR3696673.1"
                     /translation="MSETNSKNTDTLVRATAADGQIRCMAAVTTHTVAEAVRRHGASH
                     TVAAALGRTLTGTLLLGAGQKEFDRLTVQIACDGPVGGVTAETNPRGEVRGYVRNPEA
                     QAPLNANGKLDVSAVVGAGTLYVTYESGFDIGLYREPYRGSVPLVSGEIGEDFAYYLA
                     KSEQVPSAVELGVIVRAEVETGASLVEAAGGFMIQVMPGADEKIVAAIEASVPNAPRT
                     TTLIREGARPTDMLRAALGGVAFEVLEERPVSFACTCSYERVVSLITSIEPGELEAML
                     REDKGASLTCHF"
     gene            1374..>1859
                     /locus_tag="AC159P_14325"
     CDS             1374..>1859
                     /locus_tag="AC159P_14325"
                     /inference="COORDINATES: protein motif:HMM:NF012537.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide reductase N-terminal alpha
                     domain-containing protein"
                     /protein_id="HIR3696674.1"
                     /translation="MSTVIEPNSPQGSAAAAGLAGARPASSEILGLNARRVIAKRYSL
                     KDPQGNPIEEWADIVRRVVGHVGEAETEKAKRDEFYSMMLGIMLRREFVPNTPCLVNA
                     GKPAGQLAACFVLDVPDSIAGIMKTATDAAIIHQTGGGTGFTFEKIRPQGSMVRSTHG
                     VA"
BASE COUNT          325 a          654 c          582 g          298 t
ORIGIN      
        1 agaagtggca ggtgagcgag gcgcccttgt cctcgcgcag catcgcctcc aactcgcccg
       61 gctcgatgga cgtgatgagc gacaccacgc gctcgtatga gcaggtgcag gcgaagctga
      121 cggggcgctc ttcgagcacc tcgaacgcga cgccgccgag cgccgcgcgg agcatgtccg
      181 tgggccgcgc gccttcgcgg atgagcgtcg tcgtgcgcgg cgcgttcggc acggaggctt
      241 cgatcgccgc gacgatcttc tcgtccgcgc cgggcatcac ctgaatcatg aaaccgcccg
      301 ccgcctcgac gagcgacgcg cccgtctcca cctccgcccg cacgatgacg cccaactcca
      361 ccgccgaagg aacctgctcc gacttggcga ggtagtaggc gaagtcctcg ccgatctcgc
      421 ccgagacgag cggcaccgag ccgcggtaag gctcgcggta caggccgatg tcgaagcccg
      481 actcgtacgt gacgtacagc gtgcccgcgc cgaccaccgc gctcacgtcg agcttgccgt
      541 tcgcgttcaa gggcgcctgc gcctccgggt tgcggacgta gccgcgcacc tcgccgcgcg
      601 ggttcgtctc cgccgtcacg ccgcccacgg ggccgtcgca agcgatctgc accgtcagac
      661 ggtcgaactc cttctgcccc gcgccgagca ggagcgtccc ggtcagcgtg cgccccagcg
      721 ccgccgcgac cgtgtgcgag gcgccgtgcc ttcggacggc ctctgcgacc gtgtgcgtcg
      781 tgaccgcggc catgcagcga atctgcccgt cggcggcggt cgcgcgcacc agcgtgtccg
      841 tgtttttgct gttcgtctcg ctcactcgtt tcacccctct tgatactctg caaaaaggcc
      901 agcgcattag atgaaaagcg acgccttcat actgccttcg tgacactctc tacacaagag
      961 aaaaccacgc tcgaagtgtt agttcgaaaa gggccgaggg tagacgcgcg gggccgcaag
     1021 cgggggcttt cggggcgcgc gggcggctaa gcgggggctt aacgtaaaac ctcaatttat
     1081 gcgctctatt ggactgacag gaaagacaaa cgcaatatat tgtgttaggc tattgacagc
     1141 ccccaaggac gtatgtatac tgcgctccgc ttggaacgag gttgagccgc gacgcttggg
     1201 cgcccgcttc tgccccgcgc ggcacaggac tttctcgctc ccgagagcaa gccagccgca
     1261 gtaccagaaa caactcccga gcagatgacg ccgcagcctg tgcgccggcc tttttttgac
     1321 ttctattttt gagcgcctcg cgccccccac gagcgcgaga aaggcaaccg aagatgagta
     1381 ccgtgatcga gccgaacagt ccccagggaa gtgccgccgc cgccggtctc gcgggcgccc
     1441 gacccgcgtc gtccgagatt cttggcctca acgcccgccg cgtcatcgcc aagcgctaca
     1501 gcctgaaaga cccgcagggg aatcccatcg aggagtgggc cgacatcgtc cggcgcgtcg
     1561 tcggccacgt cggcgaagcc gaaaccgaga aggccaagcg cgacgagttc tactcgatga
     1621 tgctgggcat catgctccgg cgcgagttcg tgccgaacac gccgtgcctc gtcaacgcgg
     1681 gcaagcccgc ggggcagctc gccgcctgct tcgtcctgga cgttccggac tccatcgccg
     1741 gcatcatgaa gacggccacc gacgccgcca tcatccacca gacgggcggc ggcaccggct
     1801 tcaccttcga gaagattcgc ccgcaaggct cgatggtgcg ctcgacgcac ggcgtcgcc
//
LOCUS       DBNGOH010000743         2056 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3227295, whole genome shotgun sequence.
ACCESSION   DBNGOH010000743 DBNGOH010000000
VERSION     DBNGOH010000743.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2056)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3227295"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..459
                     /gene="efp"
                     /locus_tag="AC159P_14330"
     CDS             <1..459
                     /gene="efp"
                     /locus_tag="AC159P_14330"
                     /inference="COORDINATES: protein motif:HMM:NF001810.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HIR3696675.1"
                     /translation="AMVPTKLRDLRSGSSFEHRFRSADTVERASLEQHEMEYLYSDGA
                     HHHFMNTENYEQTHLNDEDLGDMAQWLTPGLKIQAEFYEGAPIGVALPPSMELTVSET
                     EPTMKGATVSNVNKPAKLENGVTITVPPFINAGDRIRVDPNEGRYIERAK"
     gene            558..1922
                     /locus_tag="AC159P_14335"
     CDS             558..1922
                     /locus_tag="AC159P_14335"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF016311.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid transferase"
                     /protein_id="HIR3696676.1"
                     /translation="MYFLYSLLLAVGVAVLLPRFLLDALRHGKYAAGLGERAGGLQRF
                     DARGRPVVWLHCVSVGETQAARPLVGALLEEFPGHALVVSTTTLTGQRVAREVFGDRA
                     ALVFYFPFDWAWSVRRALRRINPSAVLLMETELWPRFMRECRGRGVPVALVNGRISET
                     SFRRYRLIRFFIRRVVCDLSFALMQTERDAERIRALGLPRERVKVAGNIKFDADEALG
                     EQALTEDFRRRFGFDGGRPLVVAASTHAPEERVVLEAFARLAGANGAGRPRLLLAPRH
                     PERFGEVAALIESSGFAWARRSAAPKDGDAGACVILLDTIGELRAVYPLADIVFVGGS
                     ISRTGGHNVLEPAAAAKCVVTGPHTFNFTAVVNSFLERQALVQLPDLDEAEAPGALAR
                     VLGDLLANEGERRRIGERARAALEENRGATARTVGLLSTLLEQRAEVRGRGLVKSRSL
                     TSGL"
BASE COUNT          316 a          723 c          687 g          330 t
ORIGIN      
        1 gcgatggtgc cgaccaaact gcgcgacctg cgcagcggct cctcgttcga gcaccgcttc
       61 cgctcggcgg acacggtcga acgcgcctcg ctcgaacagc acgagatgga gtacctctac
      121 tccgacggcg cccaccacca cttcatgaac acggagaact acgagcagac gcacctcaac
      181 gacgaagacc tcggcgacat ggcgcagtgg ctcacgccgg gcctcaagat tcaggccgag
      241 ttctacgagg gcgcgcccat cggcgtcgcg ctcccgccgt cgatggaact gaccgtctcc
      301 gagaccgagc cgaccatgaa gggcgcgacc gtctcgaacg tcaacaagcc ggccaagctg
      361 gagaacggcg tcaccatcac cgtcccccct ttcatcaacg cgggcgaccg catccgcgtc
      421 gatcccaacg aaggccgcta catcgagcgc gcgaaataac aaaagacgta agccgtgaac
      481 cgtaaaccgt gacgggtgaa agatcacttc ttgccggcca cggtttacgg ttcacggctt
      541 acggtttacg tcttttgatg tacttcctct acagtctgct gctcgccgtg ggcgtcgccg
      601 tgctgctgcc gcggttcctg ctcgacgcgc tgaggcacgg gaagtacgcc gcagggctag
      661 gcgagcgcgc gggcggtctg caaaggttcg acgcgcgcgg gcggcccgtg gtctggctcc
      721 actgcgtgtc ggtgggcgag acgcaggcgg cgcggcctct cgtgggcgcg ctgctcgaag
      781 agttccccgg acacgcgctc gtcgtctcga cgacgacgct cacgggacag agggtcgcgc
      841 gcgaggtctt cggcgaccgg gccgcgctgg tcttctactt ccccttcgac tgggcctgga
      901 gcgtgcggcg cgcgctgcgg cgcatcaacc cgtcggcggt gctgctgatg gagacggagc
      961 tgtggccgcg cttcatgcgc gagtgccgcg ggcgcggcgt gccggtggcg ctcgtcaacg
     1021 gtcgcatctc ggagacttct ttccgccgct accgcctcat ccggtttttc atccgccgcg
     1081 tcgtttgcga tctctcgttc gcgttgatgc agaccgaacg ggacgccgag cgcattcggg
     1141 cgttgggcct cccgcgcgag cgcgtcaagg tcgcgggcaa catcaagttc gacgcggacg
     1201 aagcgttggg cgagcaggcg ttgaccgaag actttcgccg caggttcggc ttcgacggcg
     1261 ggcgcccgct cgtcgtcgcg gcgagcacgc acgcgccgga agagcgcgtc gtgctcgaag
     1321 cgttcgcgcg gctcgcgggc gcgaacggcg cggggcgccc gcgcctcctt ctcgcgccgc
     1381 gccatcccga aagattcggc gaagtcgccg cgctcatcga gtcttcgggc ttcgcgtggg
     1441 cgcgtcgctc ggcggcgccg aaagacggtg acgcgggcgc gtgcgtgatc ctgctcgaca
     1501 ccatcggcga gctgcgcgcg gtctacccgc tcgccgacat cgtcttcgtc ggcggcagca
     1561 tttcgcgcac gggcgggcac aacgtcctcg aaccggcggc ggcggcgaag tgcgtcgtca
     1621 ccggcccgca cactttcaac ttcaccgccg tcgtcaactc tttcctcgaa cggcaggcgc
     1681 tcgtccaact cccagacctc gacgaagcgg aagcgcccgg agcgctcgcg cgcgtgctcg
     1741 gcgacctgct cgcaaacgaa ggcgagcgac gccgcatcgg cgagcgcgcg cgcgcggccc
     1801 tcgaagagaa tcgcggcgcg accgcgcgca ccgtcggtct gctttccaca cttctcgaac
     1861 agcgtgcgga ggtcaggggg cgtgggctgg tcaaaagccg ctcgctgacc tccggcctct
     1921 gaccttccga gacgtcttgc ctcctatgtc ggcgtccgca ctcgcgccgc tcagccgtgt
     1981 ctccggcgcg gtggtgcgcg cgcggatggc gctctaccga agcggcagaa tcaagaccca
     2041 tcgcgccgcc gcgccg
//
LOCUS       DBNGOH010000744         4865 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3410638, whole genome shotgun sequence.
ACCESSION   DBNGOH010000744 DBNGOH010000000
VERSION     DBNGOH010000744.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4865)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4865)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4865
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3410638"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2302)
                     /locus_tag="AC159P_14340"
     CDS             complement(<1..2302)
                     /locus_tag="AC159P_14340"
                     /inference="COORDINATES: protein motif:HMM:NF016289.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HIR3696677.1"
                     /translation="MKDEVKQRTSSSSLILHPPSLSSAAALAAFLFLGAVAALSLALL
                     RPPAAVPAGAPPAEFSSGRAMKHLREIGRAPHPTGTQEHDRVREYIMRELAALGVAPE
                     VQETTAVVRAGRAAGAPFNAARVRNVVARLGGTSNTRALMLAAHYDSVPTARGATDDG
                     TGVVTLLETLRALKSGPPLRNDIIFLFTDAEELGLLGAHAFADEHPWMQDAALALNFE
                     GRGNSGPSMMFETSDGNRALVRELARAAPHTIANSLMYAVYERMPNDTDLTVFKRAGA
                     AGLNFAYTHEITHYHTMLDGPEETDERSLQHHGTYALSLARRFGDLDLGRDLPAGDGD
                     AVYFNALGSLFLHYPQSLVWPLTILSTIAFVFVIRVGLRRKLLSARGIAWGFVALLAA
                     AVAAAVATTLAWWAARRLHPGFESLPWGAPYNMGLYEVGFVLLTTALTASVYALAARR
                     ASAPNLLAGALLWWLLLLLPATVLLPSGTFIFAWPLLFSLAGLAFVLTLCEGSYAPTK
                     SLAALALCTVPCVVLVAPLVHMSFVMLGAGPAGFYMIFAVLALGLCAPLLTLLTAAKR
                     RTFPAALALAAFALVTAAILTAGFDTHRRRTSSLFYHLDAVRNEARWASTDRTLDEWT
                     SKFIAQGAIRESLADIFPWSRQQAWRGAAPALPLPAPVAEVVEDDTRGDVRRVRLRLT
                     SPRRAPLLLVHFDAETEVLSASVGGKALTGAGAGSAGASPSPLRVSFAAPPAEGIEMT
                     LEVRASRPLKLAVRDVSYELPAVPGQS"
     gene            complement(2331..2669)
                     /locus_tag="AC159P_14345"
     CDS             complement(2331..2669)
                     /locus_tag="AC159P_14345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696678.1"
                     /translation="MSDYNDFFDELIDDEAETDTQYFDDDGNEAEDDDEPAEVRTAVM
                     AKGDMLALLSLKTTDSGGQIVRYDPRETLPSARRYDDGEEALRWFRRSLSTSLKNGWH
                     VVYDGKPMFG"
     gene            2789..4426
                     /gene="glpD"
                     /locus_tag="AC159P_14350"
     CDS             2789..4426
                     /gene="glpD"
                     /locus_tag="AC159P_14350"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HIR3696679.1"
                     /translation="MARRLNGADVSACEFDAVIVGAGVNGAGLARDAAMRGLRVLLVD
                     KSDVAAGTTAWSTRLIHGGLRYLEHGELGLVRESLRERETLLRRAAPHLVKPLPLLLP
                     IYEGARRGALTVRAGMFAYDLLSFDKSLPSHRMLSRGRALRLAPGLKSEGLRGAALYH
                     DAQVEFAERLAVENALAARAHGAVVFTHARVERLVVEGGAVRGVELADLIDGGRLEAR
                     ARLVVNAAGPWVDEVLTGVADHEGQLIGGTKGSHLVVGEFEGAPRAALYAEARADGRP
                     FFIVPWDGKYLIGTTDRRYGGDLDCVEADEDEINYLLGETNRVIPSARLTRGSVLYTY
                     AGVRPLPFAREGGEAALTRRHFLREDTGGVRGLVSVVGGKLTTYRSLSEQAVGLLFKK
                     LGRPAPPCATARTPLPGAATEDFGAFAESFKASCGLAVKTCARLLKVYGVRAAEVSRL
                     AAEDERLRETVCEETGSIAAEVVFAFRDELAETLQDCLLRRTMVGLNSRAGLDVVERA
                     ARIMQQHFGWDDQRAALEIADYRQYVARFHPSPVKTP"
     gene            4423..>4865
                     /locus_tag="AC159P_14355"
     CDS             4423..>4865
                     /locus_tag="AC159P_14355"
                     /inference="COORDINATES: protein motif:HMM:NF019845.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HIR3696680.1"
                     /translation="MNNTHDSKSVPATMRALELRSYEGTEDALALVERPVPRPGKGEV
                     LVRVAASPVNPSDLMFLRGMYVRKKLPVVPGFECSGEVVSRGGGGGLLARYLVGKRVS
                     CGAPMDGDGTWAEYMVTSAALCIPLLKTTDTEQAASLIVNPFTAWA"
BASE COUNT          752 a         1491 c         1873 g          749 t
ORIGIN      
        1 ggctctggcc ggggactgcg ggaagctcgt aggaaacgtc gcgcaccgcg agtttgagcg
       61 ggcgcgaggc gcggacttca agggtcattt caatcccttc ggcgggcggc gcggcgaagg
      121 agacgcgcag ggggctcggc gaggcgcccg ccgaaccggc gccggcgccc gtgagcgcct
      181 tgccgccgac ggaggcgctc agcacttccg tctcggcgtc gaagtgaacg agcagcagcg
      241 gcgcgcggcg cggcgaggtg aggcgcaggc gcacgcggcg cacgtcgccg cgcgtatcgt
      301 cttcgacgac ttccgcgacg ggcgcgggga gcggcagcgc aggcgcggcg cccctccatg
      361 cctgctgcct cgaccagggg aagatgtccg cgaggctttc cctgatcgcc ccttgcgcga
      421 tgaacttcga tgtccactcg tcgagcgtcc ggtcggtgct cgcccagcgc gcttcgttcc
      481 taacggcgtc gaggtggtag aagaggctgc tcgtccggcg cctgtgcgtg tcgaagcccg
      541 cggtcagtat cgccgccgtg acaagggcga acgcggcgag cgcgagcgcc gcagggaatg
      601 tccgtcgttt cgccgcggta agaagcgtca ggagcggcgc gcagaggccg agggcgagca
      661 ccgcgaagat catgtagaag cccgccggcc ccgcgccgag catcacgaaa gacatgtgga
      721 cgagcggcgc gacgaggacg acgcagggca ccgtgcagag cgcgagcgcg gcgaggctct
      781 tcgtcggggc gtaactccct tcgcagagcg tgaggacgaa ggccagcccc gcgagcgaga
      841 agagcagggg ccacgcgaag atgaacgtgc cggacgggag cagcacggtc gcgggcagca
      901 gcaggaggag ccaccagagc agcgcgcccg ccaacaggtt cggcgcgctc gcgcggcgcg
      961 cggcgagtgc gtagacggag gccgtgagcg cggtcgtgag gagcacgaag ccaacctcgt
     1021 acagccccat gttgtagggg gcgccccacg ggagcgattc gaagccgggg tgcaggcgac
     1081 gcgccgccca ccacgcgagg gtcgtcgcga cggcggcggc gacggcggcg gcgaggagcg
     1141 cgacgaagcc ccacgcgatg ccccgcgccg ataaaagttt tcttcgcagc ccgacgcgaa
     1201 tgacgaagac gaaagctatc gtggagagga tggtgagcgg ccagacgaga gactgcgggt
     1261 aatgcaggaa gagcgagccg agcgcgttga agtagacggc gtcgccgtcg ccggcgggca
     1321 agtcgcgccc gaggtcgagg tcgccgaagc gtcgcgcgag cgagagcgcg taggtgccgt
     1381 ggtgttgcag gctccgctcg tcggtctctt cggggccgtc gagcatggtg tggtagtggg
     1441 tgatctcatg cgtgtaggcg aagttgagtc cggcggcgcc tgcgcgcttg aagaccgtca
     1501 ggtcggtgtc gttgggcatg cgctcgtaga cggcgtacat gagcgagttg gcgatggtat
     1561 gcggggcggc gcgggcgagt tcgcggacga gggcgcggtt gccgtcgctg gtctcgaaca
     1621 tcatggaggg gccgctgttg ccgcgtccct cgaagttgag cgcgagcgcg gcgtcttgca
     1681 tccacgggtg ctcgtcggcg aaggcgtgcg cgccgaggag gcccagctct tcggcgtcgg
     1741 tgaagaggaa gatgatgtcg ttcctgaggg gcgggccgga cttcagtgcg cgcagggttt
     1801 cgagcagcgt gacgacgccc gtgccgtcgt cggtcgcgcc gcgcgcggtc ggcacggagt
     1861 cgtagtgggc ggcgagcatc aaggctcgcg tgttcgacgt gccgccgagg cgggcgacga
     1921 cgttgcggac gcgcgcggcg ttgaagggcg cgcccgcggc tcggcccgcg cggacgacgg
     1981 cggtggtctc ctgaacctcg ggcgcgacgc cgagcgcggc cagctcgcgc atgatgtact
     2041 cgcgaacacg gtcgtgctct tgcgtacccg tggggtgcgg cgcgcgcccg atctcgcgca
     2101 ggtgtttcat cgcgcggcca gaggagaact cggcgggcgg cgcgccggcg ggcacggcgg
     2161 cgggcgggcg caggagcgcg aggctcaggg cggcgacggc gccgagaaaa agaaacgccg
     2221 ccagcgcggc ggcagaggaa agggatggag gatgaaggat gagggacgaa gaagatgtcc
     2281 tttgctttac ttcatccttc atccctccga cttcatccct tcatcgagtg tcagccgaac
     2341 atgggtttgc cgtcgtagac gacgtgccag ccgtttttga ggctggtcga gaggctgcgg
     2401 cggaaccagc gcagggcctc ttcgccgtcg tcgtagcggc gcgcggaggg cagcgtctcg
     2461 cgcgggtcgt agcggacgat ctggccgccg gagtcggtcg tcttcaggga gagcagggcg
     2521 agcatgtcgc ccttcgccat gacggcggtg cgcacctccg ccggctcgtc gtcgtcctcg
     2581 gcctcgtttc cgtcgtcgtc gaaatactgc gtgtcggtct cggcctcgtc gtcaatcagc
     2641 tcgtcgaaaa agtcgttgta gtcgctcatg ttggtggtct cgcttactcc ttgaaaaatc
     2701 gcttctctgt tcttcgactt gaggttaagg tgttaatccg catgtctcag aagcgcatga
     2761 atgtatacca gaccgcggcg aaaaccaaat ggcgcggcga ctgaacgggg ccgacgtttc
     2821 ggcgtgcgag ttcgacgcgg tgatcgtcgg cgcgggcgtg aacggcgcgg ggctggcgcg
     2881 cgacgcggcg atgcgcggcc tgcgcgtgct gctcgtcgat aagtcggacg tggcggcggg
     2941 cacgaccgcg tggtctacgc gcttgattca cggcgggctg cgctatctcg aacacggcga
     3001 gctgggcctc gtgcgcgagt ctctgcgcga gcgcgagacg ctgctccggc gcgcggcgcc
     3061 gcatctggtg aagccgctgc cgctgctgct tccgatttac gagggcgcgc ggcgcggcgc
     3121 gctcacggtg cgggcgggga tgttcgcgta cgacctgctg tcgttcgaca agtcgctgcc
     3181 gtcgcaccgc atgctctcgc gcgggcgggc gctgcggctc gcgccggggc taaagtcaga
     3241 gggcttgcgc ggcgcggcgc tctaccacga cgcgcaggtg gagttcgcgg agcggctggc
     3301 ggtcgagaac gcgctcgcgg cgcgcgcgca cggcgcggtc gtcttcacgc acgcgcgcgt
     3361 cgaacggctc gtggtcgaag gcggcgcggt ccgcggcgtc gaactcgcgg acttgatcga
     3421 cggcggaaga ctcgaagcgc gcgcacggct tgtcgtgaac gcggcggggc cgtgggtgga
     3481 cgaagtgttg accggcgtcg ccgatcatga aggacagttg atcggcggta cgaagggcag
     3541 tcacctcgtc gtcggcgagt tcgagggcgc gccgcgggcg gcgctgtacg cggaggcgcg
     3601 cgccgacggt cgcccttttt tcatcgtgcc gtgggacggg aaatatctga tcgggacgac
     3661 cgacaggcgg tacggcggcg acctcgactg cgtggaggca gacgaagacg agattaatta
     3721 ccttctcggc gagacgaacc gcgtgatacc ttccgcgcgg ctgacgcgcg gctccgtgct
     3781 ctacacctac gcgggcgtgc ggcccctgcc gttcgcgcgc gaaggcggcg aggcggcgct
     3841 gacgcgcagg cattttctga gggaggacac gggcggcgtg cgcggcctcg tctcggtcgt
     3901 cggcggcaag ctgacgacct accggagcct ctcggaacag gcggtcggtc tgctcttcaa
     3961 aaagctcggc agaccggcac cgccgtgcgc gacggcgcgc accccgctgc cgggcgccgc
     4021 cacggaagat ttcggagctt tcgcggagtc gttcaaagcg tcttgcggtc tcgcggtgaa
     4081 gacgtgtgcg cggctgctga aggtttacgg cgtgcgtgcg gctgaagtgt cgaggctggc
     4141 ggcggaagat gagcggctgc gagagactgt gtgcgaagag acgggcagca tcgccgccga
     4201 agtcgtcttc gcttttcgcg acgagctggc cgagacgttg caggactgcc tgcttcgtcg
     4261 cacgatggtc ggactgaaca gccgcgccgg gcttgacgtg gtcgagcgcg ccgcgcgcat
     4321 catgcaacag cacttcggtt gggacgatca acgcgccgcg ctcgaaatcg cggactatcg
     4381 ccaatacgtg gcacgattcc acccctcgcc ggtcaagaca ccatgaacaa cacacacgat
     4441 tccaagtccg tcccggcgac gatgcgggcg cttgagcttc gatcatacga aggcacggag
     4501 gatgcgctcg cgctggtcga gaggccggtg ccgcggcccg gcaaggggga ggtgctggtg
     4561 cgggtcgcgg cgtcgccggt gaacccttcc gacctgatgt tcctgcgcgg gatgtacgtg
     4621 cgcaagaagc tgccggtcgt gcccggtttc gagtgcagcg gcgaggtggt gtcgcggggc
     4681 ggcggcggcg ggctgctcgc gcgctacctc gtcggcaagc gcgtctcgtg cggagcgccg
     4741 atggacggcg acgggacgtg ggccgagtac atggtcacgt ccgcggccct gtgcatcccg
     4801 ctgctgaaga cgaccgacac ggagcaggcg gcctcgctca tcgtcaaccc gttcacggcg
     4861 tgggc
//
LOCUS       DBNGOH010000745         2502 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1213069, whole genome shotgun sequence.
ACCESSION   DBNGOH010000745 DBNGOH010000000
VERSION     DBNGOH010000745.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2502)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2502)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2502
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1213069"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1078)
                     /gene="xylA"
                     /locus_tag="AC159P_14360"
     CDS             complement(<1..1078)
                     /gene="xylA"
                     /locus_tag="AC159P_14360"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HIR3696681.1"
                     /translation="MATEKYQPRPEDKFTFGLWTVGNRGRDPFGDAVRPTLPPVEAAR
                     MLGEVGAYGTCLHDNDLVPIDATPQERDRIVREFKGACEENGLKVPMATVNLFYDAVF
                     RDGAFTANDAGVRAYAVQKTMRAMDIGAELGAKIFVLWGGREGVETDACRRPDEAIKR
                     LREALNYLCEYSNDQGYGYRFALEAKPNEPRADIYMPTTGAYLHFITTLDHPDMVGVN
                     PEVAHEHMPGLNMTHAVAQAWEAGKLFHIDLNDQVPGRYDQDLRFGSANPKAAFWLVK
                     FLEDVGYDGPRAFDAHAYRTEDYDGVKDFARGCMRTYLVLKEKAAQWNADAEIKALLA
                     EIAETNSGATPVGKFKKEGAQSLLS"
     gene            complement(1250..>2502)
                     /gene="galK"
                     /locus_tag="AC159P_14365"
     CDS             complement(1250..>2502)
                     /gene="galK"
                     /locus_tag="AC159P_14365"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HIR3696682.1"
                     /translation="RLLRAAGDEGCRPAARDARRAGRRGGRALSEVDKVREVFRELFG
                     GDIEPRVFRAPGRVNLIGEHTDYNDGFVLPMAIDRGTTVAAAPREDRLVRAHSVNLEE
                     TAEFDLDAAGPRRRGVWFDYVEGVAQALARRGARLRGANLVISSDVPAGAGLSSSAAL
                     EISTGLALLASSGAEVERVELALAGQEAEHTYVGTKSGIMDQFTSTFGRAGHALLIDC
                     RTLEATPIPLDTSETAVVVCDTRVKHELSTSAYNERRAECERGVELLREVLPGIKALR
                     DVSVEDFRAHEALLPEPVRRRCRHVVTEDARTRAAAEALGAGRVAELGKLMWLSHESL
                     RDDYEVSSAELDALVEIAAGLPSVAGARMTGGGFGGCTVNLVRRDALDDFRETAAREY
                     RRRFRIDLAVHVVEASDGAQEVTE"
BASE COUNT          348 a          899 c          833 g          422 t
ORIGIN      
        1 gagagagcag cgactgcgcg ccctccttct tgaacttccc gaccggcgtc gcgccgctgt
       61 tcgtctccgc gatctcggcg agcagcgcct tgatctccgc gtccgcgttc cactgcgccg
      121 ccttctcctt gaggacgaga tacgtcctca tgcatccgcg cgcgaagtcc ttcaccccgt
      181 cgtagtcttc cgtccggtac gcgtgcgcgt cgaacgcgcg gggcccgtcg taccccacgt
      241 cttcgaggaa cttcaccagc cagaacgccg ccttcgggtt cgccgagccg aaccttaaat
      301 cctgatcgta acgaccgggc acctggtcgt tgagatcgat gtggaagagc ttgcccgcct
      361 cccacgcctg cgccaccgcg tgcgtcatgt tcaagcccgg catgtgctcg tgcgcgactt
      421 cagggttgac gccgaccatg tcggggtggt cgagcgtggt gatgaagtgc aggtacgcgc
      481 ccgtcgtcgg catgtagatg tcggcgcgcg gctcgttcgg cttggcttcg agcgcgaagc
      541 ggtagccgta gccctgatcg ttggagtatt cgcagaggta gttgagcgcc tccctgagcc
      601 gcttgatggc ctcgtccggc ctgcggcacg cgtccgtctc gacgccctcg cgcccgcccc
      661 agaggacgaa gattttcgcg ccgagttcgg cgccgatgtc catggcgcgc atcgtcttct
      721 ggacggcgta ggcccggacg cccgcgtcgt tggcggtgaa ggcgccgtcg cggaagacgg
      781 cgtcgtagaa cagattgacc gtcgccatcg gcaccttgag gccgttctcc tcgcaggcgc
      841 ccttgaactc gcggacgatg cggtcgcgct cctgcggggt ggcgtcaatc gggacgaggt
      901 cgttgtcgtg caggcaggtg ccgtaggcgc cgacctcgcc gagcatgcgc gccgcctcga
      961 ccggcgggag cgtgggccgc accgcgtcgc cgaacgggtc tctgccgcgg ttgccgaccg
     1021 tccacagccc gaacgtgaac ttgtcctccg ggcggggttg atacttctct gtcgccatgt
     1081 tcttcgcctc ctcgttgtct ggtttcgtcg gatgatagca aaaagcagta ggcagtaggc
     1141 agcgggcagc aggcagtaaa aaccttctct ctcgaaggga cagggtgcaa gcgtcaacgc
     1201 cgtccgcggt cgagcgactg cctgctgcct gctgcctgct gcctgctgcc tactccgtga
     1261 cctcctgcgc gccgtcgctg gcctcgacga cgtggacggc gaggtctatc cggaagcgtc
     1321 gccggtactc gcgcgcggcg gtttcgcgga aatcgtcgag tgcgtcgcgc cgcacgaggt
     1381 tgaccgtgca gccgccgaag ccgccgcccg tcatgcgcgc gccggcgacc gacgggaggc
     1441 ccgcggcgat ctcgaccagc gcgtcaagct ccgcgctgct cacttcgtaa tcgtcgcgca
     1501 gcgattcgtg cgagagccac atcagcttcc cgagttccgc cacgcggccc gcgccgagag
     1561 cctctgcggc ggcgcgcgtg cgcgcgtcct ccgtgaccac gtggcggcac ctgcgcctga
     1621 cgggttcggg gagcagcgcc tcgtgcgcgc ggaaatcttc gacgctcacg tcgcgcagag
     1681 ctttgatgcc cggcagcact tcgcgcagga gttcgacgcc gcgctcgcac tcggcgcggc
     1741 gctcgttgta ggccgaggtg gacagctcgt gcttgacgcg cgtgtcgcag acgacgaccg
     1801 ccgtctccga agtgtcgagc gggatcggcg tcgcctccag tgtgcggcag tcgatcagga
     1861 gcgcgtggcc cgcgcgcccg aaggtggagg tgaactgatc catgatgccg ctcttcgtgc
     1921 cgacgtaggt gtgctctgcc tcctgccccg cgagcgccag ctccacgcgc tcgacctccg
     1981 cgcccgaaga cgcgagcagc gccagccccg tcgagatttc gagcgccgcc gacgaagaga
     2041 gacccgcccc cgcaggcacg tccgaagaga tgacgaggtt cgcgccccgc agccgcgcgc
     2101 cgcgtcgcgc gagcgcctgc gccacgcctt cgacgtaatc gaaccacacg ccgcgccgcc
     2161 gcggccccgc cgcgtcgagg tcgaactccg ccgtctcttc gaggttgacg gagtgcgcgc
     2221 gcaccagccg gtcttcgcgc ggcgcggcgg cgaccgtcgt gccgcggtcg atcgccatcg
     2281 gcagcacgaa cccgtcgttg taatccgtgt gctcgccgat caggttcacg cgccccggcg
     2341 cgcggaacac gcgcggctcg atgtcgccgc cgaacagctc gcgaaagact tcgcgcacct
     2401 tgtccacttc gctcaacgcc cgtccccccc gccggcccgc acggcgcgcg tcgcgcgcgg
     2461 cggggcgaca accctcgtct cccgcggccc gaagtagccg gg
//
LOCUS       DBNGOH010000746         5227 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4326865, whole genome shotgun sequence.
ACCESSION   DBNGOH010000746 DBNGOH010000000
VERSION     DBNGOH010000746.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5227)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5227)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5227
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4326865"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..647
                     /locus_tag="AC159P_14370"
     CDS             <1..647
                     /locus_tag="AC159P_14370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696683.1"
                     /translation="PSPETEEERKTKAEEAELDRVAAENGVKRPPKINTKPFEDIAQK
                     GKDLVDKGTLDLKSSTVDLSATAERNEDGTLKPETVKIEGTANNETMSTLASEFVNAL
                     SESRVLAVLEGTGQVRMSLKLDGEKVSVRIASDVASDEHASKMATGYGVLLVIARTKK
                     EGTDEGELWKNVQVGADGKQFVMSFEMSREAAAKMIADKLAKKAAAAQATPSKG"
     gene            710..1420
                     /locus_tag="AC159P_14375"
     CDS             710..1420
                     /locus_tag="AC159P_14375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696684.1"
                     /translation="MKNRSGEKMSEGLNANAARRARAGRTNSLVRFVVIGAFVFGVLP
                     APGGALLFAQPQKAGAKVNKAPRVNAAPLRAWLAQAKRQKDAGRLDLAGPRAVTIEAD
                     RAEDGTLSNAVISGPSARDPQMRKLAQDFVAALNQSRALGFLQDVSHVRMNFALDGER
                     FKTDSASDAPTAQRAEEMARGYRAMVNVARLMRRGTDEAAVLNNMKFSSNGKQLLMSL
                     DMPREQMGNLLLKQITPN"
     gene            1643..4462
                     /gene="secA"
                     /locus_tag="AC159P_14380"
     CDS             1643..4462
                     /gene="secA"
                     /locus_tag="AC159P_14380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020714251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HIR3696685.1"
                     /translation="MIDKFLTKVFGSSNQRYLKSLQPLVVEINDLEPRVKMLSDDAMR
                     ARIAELRAQVEREIAEPRDKEDLKRRTREALDKVLVEVFALTREASVRATGMRHFDVQ
                     LIGGIALHQGRIAEMRTGEGKTLVATLPATLNALTGRGVHIVTVNDYLATRDAEWMGR
                     IYKHLGLSVGAIVNDLDDWERKDAYGADITYGTNNEFGFDYLRDNMKFDLATCVQRAH
                     YYAIVDEVDSILIDEARTPLIISGASDEATDKYYKADAIIPRLKRAELNSEGKPGPGD
                     YLVDEKQHSAVLTEEGVDKAERLLAVGNLYDPSNMEMLHCVEQALKAHTLYRLDHHYV
                     VQDGQVIIVDEFTGRLMSGRRWSDGLHQAVEAKEGVKIERENQTLATITLQNYFRLYE
                     KLSGMTGTAETEAAEFDSTYKLDVATIPTNQSMIRKDNSDVIYRTLPEKWDAVVEEIK
                     ELHDKGQPILVGTVSVENSELISRRLAKVGIPHKDERGNIQIEIDGIPDHHHVLNAKH
                     HEREAEIVAQAGRKGAITIATNMAGRGTDILLGGNPEFLAREHLKRQEIDPDDPEQTT
                     PEMWAEALEFANAIVEKEHKEVVGLGGLHIIGTERHESRRIDNQLRGRSGRQGDPGSS
                     RFFLSLEDDLMRIFAGDKLKALMQRLGMEKGVALESKMVSKRIEAAQKSVEARNFESR
                     KHLLQYDDVMNRQRETIYGLRRQLMEDPDQREYILGIAQDLLSDMVKLYLGPDVGEWD
                     VENFKVRLHSVYAFDADAEGIDFEDSNPEQIEQALWEKLKANYEAKEAIVGAEVLRTH
                     ERIIMLNIIDAQWKDHLLAIDHLKQGIGLVGYGQKDPLVEYKKESFDMFQAMLDRIDT
                     TTIRTLFNLQPAVADMTPDQMRLRRLRRASRLNYTGPNQGATVAGEEAARTKTITRDQ
                     PKVGRNDPCPCGSGKKYKKCCGAAA"
     gene            4546..>5227
                     /locus_tag="AC159P_14385"
     CDS             4546..>5227
                     /locus_tag="AC159P_14385"
                     /inference="COORDINATES: protein motif:HMM:NF019339.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3696686.1"
                     /translation="MSVPHLSKYPLVFLATLLAFFAALRPSASAHTPAPLPKAATAQD
                     AQPAAQPAAPQPLLELRQAVAGERKDDPEAWFRLGVAYNVAGDAKEARKAFKQAVKLR
                     PGFIAARAGVAYTLFSEGKLKDAHKEALQATQLSPNDLASSDEAKLAFSVSVMIHTEM
                     ETRAAAAELAKAERDVSKKPNDPRRHMRKAYALLRMWPADYRVPPDLTPPERPNAASP
                     EGRAREAAL"
BASE COUNT         1032 a         1815 c         1689 g          691 t
ORIGIN      
        1 ccccttcgcc cgagaccgaa gaggagcgga agacgaaggc cgaggaggcc gaactcgacc
       61 gtgtggcggc ggagaacggc gtcaagcgcc cgccgaagat caacaccaag cccttcgagg
      121 acatcgcgca gaagggcaaa gacctggttg acaagggcac cctcgatttg aaaagctcga
      181 ccgttgacct gtcggccacc gccgagcgca acgaggacgg cacgctcaag cccgagaccg
      241 tgaagatcga gggcaccgcg aacaacgaga cgatgtccac gctcgcctcc gagttcgtca
      301 acgcgctgag cgagagcaga gtgctcgccg tgctcgaagg gacggggcaa gtccgcatgt
      361 cgctgaagct cgacggcgag aaggtctccg tccgcatcgc ctcggatgtc gcctcggacg
      421 agcacgcctc caagatggcg accggctacg gcgtgctgct cgtcatcgcg cgcaccaaga
      481 aggaagggac ggacgagggc gagctgtgga agaacgtgca ggtcggcgcc gacggcaagc
      541 agttcgtgat gtcgttcgag atgtcgcgcg aggccgccgc caagatgatc gccgacaagc
      601 tcgccaagaa ggccgccgcc gcgcaggcca cgccctcgaa gggctgaggc gggagagaag
      661 cgcgcgcacg cttgagtcaa agcccgcgcc tcgtggtaaa gttgaagaga tgaaaaaccg
      721 ctccggggaa aagatgtcgg agggcttgaa cgcgaacgcc gcccgccgcg cgcgcgcggg
      781 gcgcactaac tcgctcgtgc gcttcgtcgt catcggcgcg ttcgtgttcg gcgtgctgcc
      841 cgcgccgggc ggggcgctgc tgttcgcgca gccgcagaag gcgggcgcga aggtcaacaa
      901 ggcgccgcgc gtcaacgccg cgcccctgcg cgcgtggctc gcgcaggcca agcggcagaa
      961 ggacgcgggg cgactcgatt tggcggggcc gcgcgccgtc accatcgagg ccgaccgcgc
     1021 cgaagacggc acgctctcga acgccgtcat ctccggcccc tcggcccgcg acccgcagat
     1081 gcgcaagctc gcgcaggact tcgtcgccgc gctcaaccag agccgcgcgc tcggcttcct
     1141 gcaagacgtg tcgcacgtgc ggatgaactt cgcgctcgac ggcgagcgct tcaagaccga
     1201 ctcggcctcg gacgcgccca ccgcgcaaag ggccgaggag atggcgcgcg gctaccgcgc
     1261 gatggtcaac gtcgcgcgcc tcatgcggcg cggcacggac gaggccgccg tcctcaacaa
     1321 catgaagttc tcctcgaacg gcaagcagct cctgatgagc ctcgacatgc cgcgcgagca
     1381 gatgggcaac ctcctcctca agcagatcac ccctaactag ccatcccccg gcacgcgggc
     1441 ctgctttctt ccaagaggaa gacccgcttg cctccgcttc tgccgctcac ataaaatcgc
     1501 gcgttgcact cgtgtcgcgt cgtttcgctt gtagggtcag ggacaagtcc tcggcccgcg
     1561 ccgccgcgat agagtcaacc ttgaagggtg atgccagtcg agcgggccga ggtcatgact
     1621 cggccactac aggattactg agatgataga caaatttctg accaaagttt tcggcagcag
     1681 caaccagcgc tacctgaaga gcctccagcc gctggtcgtc gagatcaacg acctcgaacc
     1741 gcgggtcaag atgctctcgg acgacgcgat gcgcgcgcgc atcgcggagc tgcgcgcgca
     1801 ggtcgagcgc gagatcgcgg agccgcgcga caaggaagac ctcaagcgcc gcacgcgcga
     1861 ggcgctcgac aaagtcctcg tcgaggtctt cgcgctcacg cgcgaggcgt ccgtaagagc
     1921 tacggggatg cgccacttcg acgtgcagct catcggcggc atcgcgctcc atcaggggag
     1981 aatcgccgag atgcgcaccg gcgaaggcaa gacgctcgtc gccaccctgc ccgcgaccct
     2041 gaacgcgctg acgggccgcg gcgtccacat cgtcaccgtc aacgactacc tcgccacgcg
     2101 cgacgccgag tggatggggc gcatctacaa gcacctcggc ctctcggtcg gcgccatcgt
     2161 caacgacctc gacgactggg agcgcaagga cgcctacggc gccgacatca cctacggcac
     2221 caacaacgag ttcggcttcg actacctgcg cgacaacatg aagttcgacc tcgccacctg
     2281 cgtccagcgc gcgcactact acgccatcgt ggacgaggtg gactcgatcc tcatcgacga
     2341 ggcgagaacg ccgctcatca tctcgggcgc ttcggacgag gcgaccgaca agtattacaa
     2401 ggccgacgcc atcatcccgc gcctgaagcg cgcggagctg aacagcgagg gcaagcccgg
     2461 ccccggcgac tatctggtgg acgagaagca gcactcggcg gttctgaccg aggagggcgt
     2521 ggacaaggcc gagcggctct tggccgtcgg caacctctac gacccctcga acatggagat
     2581 gctgcactgc gtcgagcagg ccctgaaggc gcacacgctc taccggctcg accatcacta
     2641 cgtcgtgcag gacgggcagg tcatcatcgt ggacgagttc acgggccgcc tgatgagcgg
     2701 gcgccgctgg tcggacggcc tgcaccaggc ggtcgaggcc aaggagggcg tgaagatcga
     2761 gcgcgagaac cagacgctcg ccaccatcac cttgcagaac tacttccgcc tctacgaaaa
     2821 gctctccggc atgaccggca ccgccgagac cgaggccgcc gagttcgact cgacctacaa
     2881 gctcgacgtg gcgaccatcc cgacgaacca gtcgatgatc cgcaaggaca actcggacgt
     2941 gatctaccgc acgctgcccg agaagtggga cgccgtcgtc gaggagatca aggagctgca
     3001 cgacaagggc cagcccatcc tcgtcggcac cgtctcggtc gagaactcgg agttgatctc
     3061 gcgccgtctg gcgaaggtgg gcatcccgca caaggacgag cgcggcaaca tccagatcga
     3121 gatcgacggc atccccgacc accaccacgt cctcaacgcc aagcaccacg agcgcgaggc
     3181 cgagatcgtc gcgcaggcgg ggcgcaaagg ggccatcacg attgcgacca acatggccgg
     3241 ccgcggcacg gacatcctgc tcggcggcaa ccccgagttc ctcgcgcgcg agcacctgaa
     3301 gcggcaggag atcgacccgg acgaccccga gcagacgacg cccgagatgt gggccgaggc
     3361 gctcgagttc gccaacgcca tcgtcgagaa ggagcacaag gaggtggtgg gcctgggcgg
     3421 cctgcacatc atcggcaccg agcgccacga gtcgcgccgc atcgacaacc agctccgcgg
     3481 acgctcgggc cggcagggcg accccggctc gtcgcgcttc ttcctctctc tggaagacga
     3541 cctgatgcgc atcttcgcgg gcgacaagct gaaggcgctg atgcagcgat tggggatgga
     3601 gaagggcgtc gcgctcgaat cgaagatggt ctccaagcgc atcgaggccg cgcagaagtc
     3661 ggtcgaggcg cgcaacttcg agtcgcgcaa acacctcctc cagtacgacg acgtgatgaa
     3721 ccggcagcgc gagaccatct acggcctgcg ccgccagttg atggaagacc ccgaccagcg
     3781 cgagtacatc ctcggcatcg cgcaagacct gctctccgac atggtcaagc tgtacctcgg
     3841 ccccgacgtg ggcgagtggg acgtggagaa cttcaaagtc cggctccact ccgtctacgc
     3901 cttcgacgcc gacgccgagg ggatcgactt cgaggactcc aaccccgagc agatcgagca
     3961 ggcgctctgg gagaagctga aggccaacta cgaggcgaag gaggcgatag tcggcgccga
     4021 ggtgctgcgc acgcacgagc gcatcatcat gctcaacatc atcgacgcgc agtggaaaga
     4081 ccacctgctc gccatcgacc acctcaagca gggcatcggg ctggtcggct acgggcagaa
     4141 agacccgctc gtcgagtaca agaaggagag cttcgacatg ttccaggcga tgctcgaccg
     4201 catcgacacg acgaccatcc gcacgctctt caacctccag ccggcggtcg ccgacatgac
     4261 gcccgaccag atgcgcctgc gccgcctgcg ccgcgcctcg cgcctcaact acacggggcc
     4321 gaatcagggc gcgaccgtgg cgggcgaaga ggccgcgcgc accaagacca tcacgcgcga
     4381 ccagcccaag gtgggccgca acgacccgtg cccgtgcggc tcgggcaaga agtacaagaa
     4441 gtgctgcggc gccgccgcct gaagcggcgc ggcgcgacgc gcggggaggg gcctggcgtc
     4501 cctccccgtc aacttccctt ccgacttttt cttcgggagc ctcccatgtc cgtgccccac
     4561 ttgagtaaat acccgctcgt cttcctcgcc acgctcctgg ccttcttcgc cgcgctgcgc
     4621 ccttcggcct ccgcgcacac gcccgcgccg ctgccgaagg ccgcgaccgc gcaggacgcg
     4681 cagcccgccg cgcagcccgc cgcgccgcag cccctcctgg agttgcggca ggcggtcgcg
     4741 ggcgaaagga aggatgaccc cgaggcctgg ttccggctcg gcgtcgctta caacgtcgcg
     4801 ggcgacgcga aggaggcgcg caaagccttc aagcaggcgg tcaagctgcg ccccggcttc
     4861 atcgccgcgc gggcgggcgt ggcctacacg ctcttctcgg aagggaaact caaggacgcg
     4921 cacaaggagg cgctgcaagc cacccagctc tcgcctaatg atctcgcgtc gagcgacgaa
     4981 gccaagctcg ccttctccgt ctccgtcatg attcacacgg agatggaaac ccgcgccgcc
     5041 gccgccgagc tggcgaaggc cgagcgcgac gtgtcgaaga agcccaacga cccgcgcagg
     5101 cacatgcgca aggcttacgc gctgctacgg atgtggcccg ctgattacag ggtgccgccc
     5161 gacctcacgc cccccgaacg cccgaacgcc gcttcgccgg aaggcagggc gcgcgaggcc
     5221 gccctcg
//
LOCUS       DBNGOH010000747         3345 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2496126, whole genome shotgun sequence.
ACCESSION   DBNGOH010000747 DBNGOH010000000
VERSION     DBNGOH010000747.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3345)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3345)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3345
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2496126"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(284..853)
                     /locus_tag="AC159P_14390"
     CDS             complement(284..853)
                     /locus_tag="AC159P_14390"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component II"
                     /protein_id="HIR3696687.1"
                     /translation="MHLLVIDNYDSFTYNLVQYLGELGAGIEVRRNDEVTVEEVEARC
                     CPDKILISPGPGTPDDAGVSKALIERFAGRIPVLGVCLGHQAIGEVFGGRTVRAPAPV
                     HGKASEICHDGKTIFEGIEYRFQAGRYHSLLVERETLPDCLEISATTPDGLIMGLRHR
                     QLKVEGVQFHPESILTSEGKRLLANFLKL"
     gene            complement(990..1781)
                     /locus_tag="AC159P_14395"
     CDS             complement(990..1781)
                     /locus_tag="AC159P_14395"
                     /inference="COORDINATES: protein motif:HMM:NF024206.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HIR3696688.1"
                     /translation="MPFAFCLLISSCRDTRDERLIHPDTARRELFLRGHVYKEEDFLT
                     SAEEGDVKVVKLFLIAGMKPEVRNADAETPLMLAAKNGHVKAAQALLDKGADANARDA
                     EGHTPLTRAVIKGSAETVRLLVERGADVNAQARTGTTALMQAVANDTPKIVELLLERG
                     ADPNASDGDAGSSLAWAVYYDRPHVVELLLSKGADPNVKNNVGGTPLMIAATKGNPRI
                     VRLLLDKGADPDARDNEGKTALNWAVATGRDEVAALLQQPRPKSQ"
     gene            complement(1898..>3345)
                     /gene="trpE"
                     /locus_tag="AC159P_14400"
     CDS             complement(1898..>3345)
                     /gene="trpE"
                     /locus_tag="AC159P_14400"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HIR3696689.1"
                     /translation="VRMVLADLQTPVSAFMRVAGESPYAFLLESVEGGERVARYSFLG
                     AEPELVVRGRKDATVVERRGGGREVHEGRRAIDFMRDYFGARRLARRAGLAPMAGGAV
                     GYLAYDAVRLFEPVLEGATKVADDVDEAVFMIFRTVLAFDRVRQQIAITSVVNTDEAA
                     GDRARLEELYRAAVEETGRIEKVLAGGTEAPRADATKPEQQPEPSSFEANWTRDEFEG
                     AVRKIKEHIAAGDCYQVVLSQRLSRAVSAGPLEIYRALRATNPSPYLFFLRLGGVSVV
                     GSSPEMLVRCRGQRLDYRPIAGTRKRGATETEDWLLGEEMRADEKEVAEHTMLVDLGR
                     NDLGRVSDYGSVEVEELMAVERYSHVQHLVTSLRARLRDELDRFDALAACFPAGTVTG
                     APKVRAMQLIGELEPTPRGIYAGTVFYADYADNLDSCISIRTVVIRDRLASVQAGAGI
                     VADSVPEKEYEETLNKARALVNAVEMAERGL"
BASE COUNT          500 a         1115 c         1140 g          590 t
ORIGIN      
        1 gcgcggctca ggctctcgcc gcgcatcagc cgcaggatga aagcgcgcag cggcccgccc
       61 gcgcgcatct cctcgcgccg ctcgggcgtc ggcagcagcc acgcgggccg cgtcgtgtgc
      121 gtgttgggat cgtcggacat gatacagaag cgggcagcgg gcagcaggca tcaggcagtg
      181 caagaaagtt ccgattgagg tttgaaatct caggtcttaa gtcgtaacca ctgagttcac
      241 gatttcctgt ctttgaactg cctgctgccc gctgcctgct ctcctacagt ttcaagaaat
      301 tcgcgagcag acgtttgcct tcgctcgtca ggatcgattc gggatggaac tggacgcctt
      361 cgactttaag ctggcggtgg cgcaggccca tgatgaggcc gtcgggggtg gtggccgaga
      421 tttcgaggca gtcgggcagc gtctcgcgtt cgacgagcag cgagtggtag cgcccggcct
      481 gaaagcggta ctcgatgcct tcgaagatcg tcttgccgtc gtggcaaatc tcggacgcct
      541 tgccgtggac gggcgcgggc gcgcgcaccg tgcgcccccc gaagacctcg ccgatggcct
      601 ggtgtccgag gcacacgccg aggacgggga tgcggcccgc gaagcgctcg atgagggcct
      661 tcgagacgcc cgcgtcgtcg ggcgtgcccg gccccggcga gatcaggatt ttgtcggggc
      721 agcagcgggc ctcgacctcc tcgaccgtca cctcgtcgtt gcgccgcacc tcgatgcccg
      781 cgcccagctc gccgaggtac tggacgaggt tgtaggtgaa cgagtcgtag ttgtcgatga
      841 cgagtaggtg catgacgtta tcactcggat tgcggatttg ggattgcgga ttgcggatta
      901 atcgaccgac aaaaggtgaa tgtcagaaat caagcatcaa gttcaagcgg cattccaatc
      961 cgcaatccgc aatcccaaat ccgcaatcgt tactggctct tcgggcgcgg ctgttggagc
     1021 agggcggcca cttcgtcgcg cccggtggcg acggcccagt tgagggcggt cttgccttcg
     1081 ttgtctctgg cgtcggggtc ggcgcctttg tcgagcagca gtcgcacgat gcgcgggttg
     1141 cccttcgtcg cggcgatcat gaggggcgtg ccgccgacgt tgtttttgac gttcgggtcg
     1201 gcgcccttcg agagcagcag ctcgacgacg tgcgggcggt cgtagtagac ggcccacgcc
     1261 agcgagctgc ccgcgtcgcc gtcgctcgcg ttcgggtcgg cgccgcgttc gagcagcagc
     1321 tcgacgatct tcggcgtgtc gttggccacg gcctgcatga gggcggtcgt ccccgtcctg
     1381 gcctgcgcgt tcacgtcggc gccgcgttcg acgagcaggc ggacggtctc cgccgagcct
     1441 ttgatgacgg cgcgcgtgag cggcgtgtgg ccttcggcgt cgcgcgcgtt ggcgtcggcg
     1501 cctttgtcga gcagcgcctg cgcggccttc acgtggccgt tcttcgccgc cagcatgagc
     1561 ggcgtctcgg cgtcggcgtt cctcacctcc ggcttcatcc ccgcgatgag gaagagcttg
     1621 acgactttca cgtcgccctc ctcggcgctc gtgagaaagt cctcctcctt gtagacgtgg
     1681 ccccgcagga acagctcgcg ccgcgccgtg tccgggtgga tgagacgttc gtcgcgcgtg
     1741 tcgcggcagg aggagatgag aaggcaaaag gcaaaaggca agaggcaaag ggcaaaagtg
     1801 tggacacgcc tccggcgtga gctttcgaat acagaaagcg aagcggagct tcgtccgccc
     1861 ttttgccttt tgccttttgc cttttgcctt tcgcctttca tagcccccgc tccgccatct
     1921 cgaccgcgtt gacgagcgcg cgcgccttgt tcagcgtctc ttcgtactcc ttctcgggca
     1981 ccgagtcggc gacgatgccg gcgccggcct gcaccgaggc cagtcggtcg cggatgacga
     2041 cggtgcggat cgagatgcac gagtcgaggt tgtcggcgta gtcggcgtag aagaccgtgc
     2101 ccgcgtagat gccgcgcggc gtgggttcca gctcgccgat gagctgcatg gcgcgcacct
     2161 tgggggcacc cgtgacggtg ccggcgggga agcaggcggc gagcgcgtcg aagcggtcga
     2221 gttcgtcgcg cagccgcgcg cgcagggaag tgacgaggtg ctggacgtgc gagtagcgct
     2281 cgacggccat cagctcttcg acctcgaccg agccgtagtc cgagacgcgc cccagatcgt
     2341 tgcgcccgag atcgacgagc atcgtgtgct cggccacctc cttctcgtcg gcgcgcatct
     2401 cctcgccgag cagccagtct tcggtctcgg tcgcgccgcg cttgcgcgtc cccgcgatgg
     2461 gccggtagtc gagccgctgc ccgcgacacc tgaccagcat ctccggcgac gagccgacga
     2521 ccgagacgcc gccgaggcgc aggaagaaga ggtacggcga ggggttcgtc gcgcgcagcg
     2581 cgcgatagat ttcgagcggc ccggcggaga ccgcgcgcga caggcgttgc gagagcacga
     2641 cctgatagca gtcgcccgcc gcgatgtgct ccttgatttt gcgcaccgcg ccttcgaact
     2701 cgtcgcgcgt ccaattggcc tcgaacgacg aaggctcggg ctgctgctcc ggcttcgtcg
     2761 cgtcggcgcg cggcgcttcc gttccgcccg cgagaacctt ttcgatgcgc ccggtctcct
     2821 cgaccgccgc gcggtacagc tcttcgaggc gcgcgcggtc gcccgccgcc tcgtccgtgt
     2881 tgacgacgga agtgatggcg atctgctggc gcacgcggtc gaacgcgagc accgtgcgga
     2941 agatcatgaa gaccgcttcg tccacgtcgt ccgcgacctt cgtcgcgcct tcgagcaccg
     3001 gctcgaacaa gcgcaccgcg tcgtaggcga ggtagccgac ggcgccgccc gccatcggcg
     3061 ccagccccgc gcgccgcgcc agcctgcgcg cgccgaagta gtcgcgcatg aaatctatcg
     3121 cgcgcctccc ctcgtggact tcgcgcccgc cgccgcgccg ctcgacgacg gtggcgtcct
     3181 tccgtccgcg cacgaccagc tccggctccg cgccgaggaa cgagtagcgc gcgacgcgct
     3241 cgccgccctc gaccgattcg agcaggaacg cgtacggcga ctcgcccgcc acgcgcatga
     3301 acgcgctgac gggcgtctgc aagtcggcca gcaccatccg cacca
//
LOCUS       DBNGOH010000748         2108 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1030472, whole genome shotgun sequence.
ACCESSION   DBNGOH010000748 DBNGOH010000000
VERSION     DBNGOH010000748.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2108)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2108)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2108
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1030472"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            185..916
                     /locus_tag="AC159P_14405"
     CDS             185..916
                     /locus_tag="AC159P_14405"
                     /inference="COORDINATES: protein motif:HMM:NF024183.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HIR3696690.1"
                     /translation="MSTNKCPSCGLVNFRSEPQCLRCAAPLPGGGGQTPFLGCTFCGS
                     QLSVTAQHCPACNTPVRATVEPPRPVAAQPAAERAPRTPEGPLVAEHEPRAPQRSLAP
                     CPDCGHMVSTAAASCPSCGRPFTQAAPASPFQHMPRPVPYAPQPVQYIAPAVQYAQQV
                     IVAPAYQPALMTPPKSRGIYIVLALFLGGLGVHNFYAGYNGRGAAQLVITLVTGLSGG
                     FFLVAIWAFVECFIINTDAYGRRMT"
     gene            complement(990..1382)
                     /gene="ssrA"
                     /locus_tag="AC159P_14410"
     tmRNA           complement(990..1382)
                     /gene="ssrA"
                     /locus_tag="AC159P_14410"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            1690..>2108
                     /locus_tag="AC159P_14415"
     CDS             1690..>2108
                     /locus_tag="AC159P_14415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020264189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HIR3696691.1"
                     /translation="MAAKLTRPAANGKNRPAPKRRQLLRRFWLPAVLLLAIAAGGLTG
                     IVLAYGLNYSRAAEEVKALATYRPSVVTRVYADDEKTVIGEFALERRIPLKHEEIPPV
                     VKNAILAVEDVRFYDHMGIDPIRTVGAAWKNLTTDKV"
BASE COUNT          358 a          754 c          631 g          365 t
ORIGIN      
        1 acgaagggcg actttcggcc ccgcggcccg cggaaaatta acggttgcgc acattcgccg
       61 cacgccttac actcttaaag cgcggcccac accgccgcgc cctgcccggc ccggagcagt
      121 tttcgtcacg cggcccttac gacccgtctt caacatcaat cgaacgcgag tgagtttccg
      181 aaccatgtcc accaacaaat gcccgagctg cgggctggtc aacttccgct ccgagccgca
      241 atgcctgcgg tgcgcggcgc cgctgcccgg cggcggcggg cagacgccct tcctcggctg
      301 cacgttctgc ggctcgcagc tctccgtcac ggcccagcac tgccccgcct gcaacacgcc
      361 cgtccgcgcg accgtcgagc cgccgcggcc cgtcgcggcg cagcccgccg ccgaacgcgc
      421 gccgcgcacg ccggaggggc cgctcgtcgc cgagcacgag ccgcgcgcgc cgcagcgcag
      481 cctcgccccc tgccccgact gcgggcacat ggtttcgacc gccgccgcgt cttgcccttc
      541 gtgcggcagg ccgttcacac aggccgcgcc cgcctcgccg tttcagcaca tgccgcggcc
      601 cgtgccgtac gcgccgcagc cggttcagta catcgctccc gccgtgcaat acgcgcagca
      661 ggtgatcgtc gcgcccgcgt accagccggc gctgatgacc ccgccgaaga gccgcggcat
      721 ctacatcgtg ctggccctct tcctcggcgg cctcggcgtc cacaacttct acgcgggcta
      781 caacggcagg ggcgcggcgc agctcgtcat cacgctcgtc acgggactca gcggcggctt
      841 ttttctggtc gccatctggg ccttcgtcga gtgcttcatc atcaacacgg acgcgtacgg
      901 tcggcggatg acttgagcga caggcaagtt tttgtgagac gaaaaaaagc agagcctctg
      961 ttcacagcag gctctgcttg ttttcttgcg aatggaggtg ccgagaatcg aactcgggtc
     1021 caaaggccga cgccgtcgga atctacatgc ttagacgctt ctctgttgcc ggactcgctc
     1081 cggcgcgttc gccccgcgcg ggtcagtgaa gcgacgaaga ccccgcccgc ggctagccca
     1141 gatttttctc gcccgccttc cctgagcacg gaagccgggc cagctcaact gagtgacatc
     1201 tcgctccgcg cgcttgagca acttgcggtg agacgctcgc aagacctaat tacttaggcg
     1261 gcgagagcca tttcgttgga attggcaatt gtgtttctca ctgcttttta acgagctgca
     1321 tagagtaagc ccggcatgca tccgtccgat gttcatagcc cctgtcgaaa cctgtcaccc
     1381 ccttgtaaag gcgtgcaacc tatcggatgc acgggggggt attatagatg cctaaaccta
     1441 atagaatcaa gccatccgcc gcgctcccgc ccgcatccaa ttcgctcgac cggcgcgcat
     1501 cgaactctct ttgatcgtgg ctacccacac caccgcgacc gtgtgtgcgc ctcgcgcggg
     1561 tggcgcgcgt tgggctataa ttcgcgacgc gcgcggcaat ctcccgccgc gcactccttg
     1621 gtcgaatcgg gtctgtagag cgcaacgcgc ccgcctgacg cgcgttcctt ccggagaggt
     1681 tagaaattca tggcagcaaa acttacgcgt cccgccgcca acggcaagaa ccgtcctgcg
     1741 ccgaagcgcc gtcaactgct ccgccgcttc tggctgccgg cggtgctcct gctggcgatt
     1801 gccgccgggg gcctgacagg catcgtgctg gcctacggcc tgaactactc gcgcgcggcg
     1861 gaagaggtga aggcgctggc gacctacagg ccgagcgtcg tgacgcgcgt ctacgcggac
     1921 gacgagaaga cggtcatcgg cgagttcgcg ctcgaacggc gcatcccgct caagcacgag
     1981 gagattccgc ccgtcgtcaa gaacgccatc ctcgccgtcg aggacgtgcg cttctacgac
     2041 cacatgggca tcgacccgat ccgcacagtg ggcgcggcgt ggaagaacct gacgaccgac
     2101 aaggtcga
//
LOCUS       DBNGOH010000749        11253 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4510889, whole genome shotgun sequence.
ACCESSION   DBNGOH010000749 DBNGOH010000000
VERSION     DBNGOH010000749.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 11253)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11253)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11253
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4510889"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(79..3426)
                     /locus_tag="AC159P_14420"
     CDS             complement(79..3426)
                     /locus_tag="AC159P_14420"
                     /inference="COORDINATES: protein motif:HMM:NF028438.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3696692.1"
                     /translation="MKGTVTYGISKALRAAALCALVLASAADGRAQSDRSPRVWEAPL
                     SIPTYELGAPDPNPALLDWNRRKWRPVYPYAMMDTLTTKRVEKSYKAVYLENEYLKVT
                     VLPELGGHVYAIFDKTASRDVLYTNHVVKYAMVAIRGAWVSGGIEWNFPDGHTLTTVS
                     PIDYATRMEADGSAAVVIGDTERVQRMSWSIICRLRPGSKALETEVTLNNRRETPGRY
                     WYWSTAAAPATEDMRFVYPMRESYPHAFWPVYSFPKEKGVDIGTYREVKNELSLFARN
                     SKRDFFGVYYEKSDWGIVHVADHKELPGKKTWTWGTDESGRIWVDKLTDADGQYVEFQ
                     AGRFETQMEHEFIAPHRVERFTEYWFPVNRMGGGFDEATRDAAVNVNVEGQRATVIMN
                     ANAKFDDAGLTVEAGGRRLHTDSVDLDPAKAHTASFNIPADAAGQPLVVTLRAKDGRQ
                     LLRYSTDAPADGNADFKPATRPRPDPKAPASAEQAYVEGLAADKKSNERVARAAFQEA
                     LKRDAGFAPAHTALGLSYYRSGEYERATGHLEAALRRNKDAGDAHYYLGLVRRAEGRS
                     VEASEHLMWAVRAGHRESVARYVLGEMALAAGDTASALEHLQQAVLLDPRDLKARTVL
                     ALAERAAGRLESAQQRIDAVVWEAPLDYLALAEQSEIHRARGNDAKADGARGQLWRLL
                     SREPDSVLELAFDYLAAGRLGEGRGVLEEGIRRAGAQGARAFPMLHYALGYFHEKAGD
                     TARAREQYALGSQGDTAFVFPHRVEELAVLRAAAAANPSDGRAAYYLGNVLASKGRGE
                     EALTAWRDAVRLDPSNGVARRNLARALWLVAGKKDEAASEYERAIKAAAEDFHLYVEL
                     DRLLAEMNATPRRIRLLEGAPESVRRRSPVVQATAAAYVEAGRFGDAAALLERTQFTS
                     GEGEFAALGIFRRAHIGLAREHQRAGRHAEAAREFLRATEYPKNLGAGAPSMQSQARE
                     YVAAAGEFEAAGVRDEAERLWRRAADEPLNSPTQPTEPWSEHYYFKAVALERVGRREE
                     ARALYQRLANLNDEARMLEAEPAPPEGAIRYVLAGAGLKALGRTAEARAALERALKMD
                     PQNELAKTQLAELSAPARGRAKTGSGR"
     gene            complement(3610..4758)
                     /gene="menC"
                     /locus_tag="AC159P_14425"
     CDS             complement(3610..4758)
                     /gene="menC"
                     /locus_tag="AC159P_14425"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: protein motif:HMM:TIGR01928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HIR3696693.1"
                     /translation="MVKAKEEAGVAPLRIERVALHRVRVPLCEPFRISNGSVAEKDSV
                     LIEVTTDRGVTGWGESSPMSGSFYSDDTPETVWRALSETLVPLALGADEIDAPRFYER
                     LRAVPGEAFAKAGLEGALWDAHARTLGVPLREALGARPRPVPSGLAVGIYDTVEELVE
                     RVGRYLAEGYRRVKIKIQPGWDVVPVEAVRARFPGVPLMVDANAAYTLADADIFRRLD
                     AYELMMFEQPLARGAHEDSAELQRQLRTPVCADESADSLADVEQIIRLGAARIVNIKL
                     QRVGGLSEARLMLARVRAAGLGCWLGTMPELGVASAQGLHLAALAGFDYPTDIEASAR
                     WFVDDIIEPQIEIDREGFIIPPSGAGTGYEVSREKVRAHAVESREFTR"
     gene            complement(4781..5827)
                     /locus_tag="AC159P_14430"
     CDS             complement(4781..5827)
                     /locus_tag="AC159P_14430"
                     /inference="COORDINATES: protein motif:HMM:NF014841.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HIR3696694.1"
                     /translation="MSEERTETNEVEGVSTLARPRILVLTSDALFPHFFPAHVLARLE
                     EVGEWEHYDGREDSAELREHLSRSDALMTTWHSPFLRMEMFAQPRRVRLIAHCGGELK
                     ARMEPEVVERLTVANAPGPMAAPVAEMALAMVLTLVRQLPRYDREMRAGLVQSNEVVS
                     RGETVRGRRVGIVGFGRIGRAFARMVAPLGAELYVSDPYCSDETAAEYGAKKAETEEL
                     LRTSSVVVLAAGLTDETRGLLDARTLALMPDGAYLVNVGRGGLIEMEALLSELRTGRL
                     SAALDVTDPFEPLPPEHELRRLPNVLLTPHIAAGGIEMRHALGETAAAEVARFFRGER
                     LENVVTPEMLARMT"
     gene            complement(5863..6696)
                     /locus_tag="AC159P_14435"
     CDS             complement(5863..6696)
                     /locus_tag="AC159P_14435"
                     /inference="COORDINATES: protein motif:HMM:NF013429.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HIR3696695.1"
                     /translation="MLKLAFSTLGCPNWELEEVARAAREYGYEGVELRALGGGLDWPA
                     RPEFQPGEVARTREWLAGRGVDICCVDTSCTFDSVDESERRRQVELAGRHCELAAALG
                     APLIRVFPDLVPEGATREQTRDRISDSLREVARLAPRGVRVGLETHGDFARGHMTADI
                     IRRADHPNLCIIWDAANTFASGEEVEESSAAVAPFLAHVHLRDARPMGKEHWTPVLAG
                     RGLVPFERVVAALRKLDYRGYVSFEFEKYWHPEIEEPDVSLPDFAAAMRPLPGGQAGA
                     A"
     gene            complement(6713..8026)
                     /locus_tag="AC159P_14440"
     CDS             complement(6713..8026)
                     /locus_tag="AC159P_14440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696696.1"
                     /translation="MRYFVACLFLAACFALSGAADSVQAATYQVGPSRTYTKLQDVAP
                     LLAPGDLVEVDGNVTYPGGIIFTVPGTPTNKITIRGLRVNGQRPVLSGGTNTVEFRLS
                     NHYVFEGFDVTGGSFRGIYHHAHDITIRDTVVHDCPAHGILGADTDSGSLTLEYVEVY
                     NCGNGTGQHPVYMATDEFKYPGAVFRMQYCYLHDQRGGNNIKSRAERNEIYYNWVSGG
                     FYGEIELIGPDPKGNRNVSTDLKREDSDVVGNVFQHKATQGTRMVRVGSDGTGQTKGR
                     YRFVNNTFILRQGQVSPAFEIFEGIDSLEVHNNVFYRPGNEPIDKVVLETRVRWVAGR
                     VSVGTNNWLPFGSAMIPTDWTNTVFGTDPGFANLTTLDLRPAVGSALIGAGNNAPASP
                     AGHPFINPLWPPAFHPPLHTLPAPGTAAARPSDGLIDIGAYEYAP"
     gene            complement(8061..9890)
                     /locus_tag="AC159P_14445"
     CDS             complement(8061..9890)
                     /locus_tag="AC159P_14445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696697.1"
                     /translation="MRKLATLLSLICLFQNLALAGQPARVWAVSDGEKVERDDLKNRS
                     RASNSAWDGRRIKIFGARNEVIAFQVVVEAGADGIGALSVALPELSKRGGGESIRYAR
                     PSADPTDYAGRPIQLFSVNYMNVTETTRANWVWRPGSASAPADTLGWKPVQLVPENAK
                     AGRGGFPLKVSPRRNQAVWIEIYTARDLPAGVYEGRVEVTADGRRQTLPVELELFDFT
                     LPDENSMHAMVYYEPLQPELYQGRNLDPAYHRFAHRHRVELVHAYSVESARAARARFT
                     GEDFTPARGYEGPGESVGNRVIPRSFYGPGKDFDERESAWRNSDEWMAFLRENFPAAL
                     TFLYMPDEPGPSEYEYIRRLAENVHSNPGPGKSLPIFVTKRYTKELDGAIDIWDSGPQ
                     GFDIEQVRRERERGRRYWIYNGGRPAAGAIVIDAPATDPRATIWACFKHGIEVYFYWH
                     GVHWRHNSQKQGVREQNVWANPVTFDNRGQPRKPAGSQGWINGDGVLMYPGEEKLHPA
                     EDRGLHGPVSTVQLANFRRGLQDHQYLTLARKAGLNAEVEAAVRAVVPRVFSEVPRGG
                     AVSFAETGDRFESVRYALARSIARAPRDDSPRPRPSRGKRRGH"
     gene            complement(9995..>11253)
                     /locus_tag="AC159P_14450"
     CDS             complement(9995..>11253)
                     /locus_tag="AC159P_14450"
                     /inference="COORDINATES: protein motif:HMM:NF012686.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sodium:solute symporter family transporter"
                     /protein_id="HIR3696698.1"
                     /translation="LAYTVLGGYWAVCFTDMFQFMFLFPVALLLMILSLQDVGGVSGF
                     VAQAPEGYTNPFAGEYGWLFLVAYTISQTVGYNNFGNAQRYFCAPTERDARRIAVLCM
                     ALFTLGAFVFYTPPLVARVVMPELATAANGLKQPTEAAYISMGMRLLPPGLVGVVLAA
                     MLASSMASLSAINHVVTGILAKDLYQGYVRRDATEKHMLTASRLASLLVGVVMIVVAL
                     VFAMGTTGIFTLLFILESIFLLPLGLPLLYGMLVKRGPWWSAVFAYSVGAATSLFVNF
                     YLNRYLGWGLNETYLIGVPAITTTVGFFVPVLLIKPTGEFAGRVSAFFKKLATPISPA
                     TELGESGMSGRGQLAIVGKVTTGIGLACFLMLLANTSGRELLITAAYAVVTTVIGLAF
                     VVAGRTPQGVRGRVAAAVGAEAAAKD"
BASE COUNT         1685 a         3913 c         3871 g         1784 t
ORIGIN      
        1 ttcggcggcc ccggccttga aaggccgggc taaactcaag cgccgctccg cggcgattca
       61 tgcgcacgct tcaactaatc agcgcccgct ccccgtcttc gcgcgaccgc gcgccggcgc
      121 ggacagctcc gcaagctggg tcttcgccag ctcgttctgc ggatccatct tcagcgcgcg
      181 ttcgagcgcg gcgcgcgcct ccgccgtgcg accgagcgcc ttgagaccgg cgccggccag
      241 aacgtaccgg atcgcgccct cgggcggcgc aggctccgcc tcaagcatcc gcgcctcgtc
      301 gttcaggttc gcgagccgct ggtagagcgc ccgcgcctcc tcgcgccggc ccacgcgttc
      361 gagcgcaacc gccttgaagt agtagtgctc cgaccagggc tcggtcggct gcgtcggcga
      421 gttgagcggc tcgtcggcgg cgcggcgcca gagcctctcg gcctcgtcgc gcacgcctgc
      481 ggcctcgaac tcgccggcgg cggcgacgta ctcgcgcgcc tgcgactgca tcgaaggcgc
      541 gcccgcgccg agattcttcg ggtactcggt cgcgcgcagg aactcgcgcg ccgcctcggc
      601 gtggcgcccc gcgcgctggt gctcgcgcgc cagcccgatg tgcgccctgc ggaagatgcc
      661 gagcgcggcg aactcgccct cgcccgaggt gaactgcgtc cgctccaaaa gcgccgccgc
      721 gtcgccgaag cgcccggcct cgacgtaggc ggcggcggtc gcctgcacga ccggggagcg
      781 tcgtcgcacg gactcgggcg cgccttcgag cagacggatg cggcgcggcg tggcgttcat
      841 ctcggcgagc agacggtcaa gctcgacgta gaggtgaaag tcttccgcag cggccttgat
      901 ggcgcgctcg tactcggacg ccgcctcgtc cttcttcccc gcgacgagcc agagggcgcg
      961 cgcgaggttg cggcgggcga cgccgttcga cgggtcgagc ctcaccgcgt cgcgccacgc
     1021 ggtcagcgcc tcctcgccgc gccccttcga ggccaggacg ttgccgaggt agtaggccgc
     1081 gcgcccgtcc gaagggttcg ccgctgcagc cgcccgcagc accgccaact cctcgacgcg
     1141 gtgcgggaag acgaaggccg tgtcgccctg cgacccaagc gcgtactgct cccgcgcgcg
     1201 cgccgtgtcg cccgctttct cgtggaagta gccgagcgcg tagtggagca tggggaaggc
     1261 acgcgcccct tgcgcgcccg cgcgcctgat cccctcttcg agcacgccgc gcccttcccc
     1321 gaggcggcca gccgcaagat agtcgaaggc gagttccagc accgagtctg gctcgcgcga
     1381 gagcagccgc cacaactgcc ctcgcgcccc gtcggccttc gcgtcgttcc cgcgggcacg
     1441 gtgaatctcg gactgctcgg cgagcgccag atagtcgagc ggcgcctccc agacgaccgc
     1501 gtcgatgcgc tgctgcgccg actcgagccg ccccgccgcg cgctcggcca acgcgagcac
     1561 ggtgcgcgcc ttgaggtcgc gcgggtcgag cagcaccgcc tgctgcaagt gttcgagcgc
     1621 actggccgtg tcgcccgcgg ccagagccat ctcgccgagg acgtagcgcg cgaccgactc
     1681 gcggtggccc gcacggacgg cccacatcag gtgctcggac gcctcgacgc tgcgcccctc
     1741 ggcgcgccgc acgaggccga ggtagtagtg cgcgtcgccc gcatccttgt tgcggcgcag
     1801 cgccgcctca aggtgcccgg tcgcccgctc gtactcgccg ctgcggtagt aggaaagccc
     1861 gagcgccgtg tgcgcgggcg cgaagcccgc gtcgcgcttg agcgcctcct ggaaggccgc
     1921 gcgcgccacg cgctcgttgc tcttcttgtc ggcggccagc ccctcgacgt acgcctgctc
     1981 ggcgctcgcc ggtgctttcg ggtcgggccg cgggcgcgtg gccggcttga agtcggcgtt
     2041 gccgtccgcg ggcgcgtcgg tgctgtaacg cagcaactgc cgtccgtcct tcgcgcgcag
     2101 cgtgacgacg agcggctggc ccgcagcgtc ggcagggatg ttgaaagagg ccgtgtgcgc
     2161 cttcgccggg tcgaggtcaa ccgagtccgt gtggagacgg cgcccgccgg cctcgaccgt
     2221 cagccccgcg tcgtcgaact tcgcgttcgc gttcatgatg accgtggcgc gctggccttc
     2281 gacgttgacg ttgacggcgg cgtcgcgcgt cgcctcgtcg aagccgccgc ccatgcggtt
     2341 gacggggaac cagtattcgg tgaagcgctc gacgcggtgc ggggcgatga actcgtgctc
     2401 catctgcgtc tcgaagcgcc cggcctgaaa ctcgacgtac tggccgtccg catcggtcag
     2461 cttatcgacc cagatgcgcc cgctctcgtc cgtaccccac gtccacgtct tcttgcccgg
     2521 cagctccttg tggtcggcga cgtggacgat gccccagtcg gacttctcgt agtagacgcc
     2581 gaagaagtcg cgcttcgagt tgcgtgcgaa cagcgacaac tcgttcttca cctcgcggta
     2641 ggtgccgatg tccacgccct tctctttggg gaaggagtag acgggccaga aggcgtgcgg
     2701 gtagctctcg cgcatcgggt agacgaagcg catgtcttcg gtcgcgggcg cggcagccgt
     2761 ggaccagtac cagtagcgac cgggcgtctc gcgccggttg ttgagcgtga cctcggtttc
     2821 gagcgccttg ctgcccggcc tgaggcggca gatgatagac cacgacatgc gctgcacgcg
     2881 ctcggtgtcg ccgatgacga ccgccgcgct gccgtcggct tccatccgcg tcgcgtaatc
     2941 gatcggcgag acggtggtca gcgtgtggcc gtcggggaag ttccactcga tgccgcccga
     3001 gacccacgcg ccgcggatgg cgaccatcgc gtacttgacg acatggttcg tatagagcac
     3061 gtcgcggctc gccgtcttgt cgaagatggc gtagacgtgc ccgccgagtt cgggcagcac
     3121 cgtcaccttc aggtactcgt tctcaaggta gacggccttg tagctcttct ccacgcgctt
     3181 cgtcgtcagc gtgtccatca tcgcgtaggg gtagacggga cgccacttcc gacgattcca
     3241 gtccaggagc gccgggttcg ggtcgggcgc gcccaactcg taggtcggaa tcgagagcgg
     3301 cgcctcccag acgcgcggcg atctgtcgga ctgcgcgcgc ccgtcagccg cggaggcgag
     3361 cacgagcgcg cagagtgccg ccgcccgcag ggctttggaa attccgtaag tgactgttcc
     3421 cttcataaga cccctctgca tatcccttcg gccaaattac ttgaaagctg tgcacaaatc
     3481 cgccgcgacg cggtgatgcc tcattcatct caacccggcc ttgaaaggcc gggctaaact
     3541 cagacgccgc tccgcggcga tttacgcaca tgcttcgact tacttaacga taaatccgtc
     3601 ggcgaacctt cagcgcgtga actcgcgcga ctcgacggcg tgcgcccgaa ccttttcacg
     3661 cgagacctcg tatcccgtcc ccgcgcccga gggcggaatg atgaagccct cacggtctat
     3721 ctcaatctgc ggctcgatga tgtcgtccac gaaccagcgc gcggaggctt cgatgtcggt
     3781 cgggtagtcg aagcctgcga gcgcggcgag gtgaagcccc tgcgccgagg cgacgcccaa
     3841 ctccggcatc gtccccagcc agcagccgag tcccgccgcg cgcacccgtg cgagcatcag
     3901 gcgcgcctcg ctcaagcccc cgacgcgttg aagcttgatg ttgacgatac gcgccgcgcc
     3961 cagcctgatg atctgctcga catccgcgag cgaatcagcc gactcgtcgg cgcagacggg
     4021 cgtccgcaac tgccgctgca actccgcgga gtcttcgtgc gcgccgcgcg ccagaggctg
     4081 ctcgaacatc atcaactcgt acgcgtcgag gcggcggaag atgtccgcgt ccgcgagcgt
     4141 gtaagcggcg ttggcgtcca ccattagcgg cacgccgggg aaacgcgccc ggacggcctc
     4201 gaccggcacc acgtcccaac ccggctgtat ctttatcttc acccgccggt agccttcggc
     4261 gaggtagcgc ccgacgcgct cgaccagttc ttcgaccgtg tcgtagatgc cgacggcaag
     4321 ccccgaaggc acgggccggg gccgcgcgcc gagggcctcg cggagcggca cgcctaacgt
     4381 ccgagcgtgc gcgtcccaca gcgcgccttc gagtccggcc ttcgcgaacg cctcgcccgg
     4441 caccgcgcgc agccgctcgt agaaacgcgg ggcgtctatc tcgtccgcgc cgagcgcgag
     4501 cgggaccagc gtctccgaga gcgcgcgcca gaccgtctcg ggcgtgtcgt ccgaatagaa
     4561 cgagccggac atcggcgacg actcgcccca gcccgtcacg ccccggtcgg tcgtcacttc
     4621 gatcaggacg gaatccttct cggccaccga gccgttgctg atgcggaacg gctcgcacag
     4681 cggcacgcgc acgcggtgta gcgcgacgcg ctcgattctc agcggggcga cgcccgcctc
     4741 ttcttttgct tttaccatga tcgaatcgac gcagtgcgct tcaggtcatc cgcgccagca
     4801 tctcgggcgt gacgacgttt tcgagtcgct cgccgcggaa gaagcgcgcg acctctgcgg
     4861 cggccgtctc gccgagcgcg tggcgcatct cgatgccgcc ggctgcaatg tgcggcgtga
     4921 gcaggacgtt cggcagccgg cgcagctcgt gttcgggcgg gagcggctcg aaggggtcgg
     4981 tcacgtcgag cgccgccgaa agtctccccg tgcgcaactc ggagagcagc gcctccatct
     5041 cgatcagccc cccgcgcccg acgttgacga gataggcgcc gtccggcatc agcgcgagcg
     5101 tccgcgcgtc gagcaggccc cgcgtctcgt cggtcagccc cgccgcgagc acgacgacgc
     5161 tcgacgtgcg cagcagttct tccgtctccg ccttcttcgc gccgtactcc gccgccgtct
     5221 cgtccgagca gtatgggtcc gagacgtaca gctccgcccc gagcggggcg accatgcgcg
     5281 cgaaggcgcg cccgatgcga ccgaagccca cgatgccgac gcgtcggcca cgcacggtct
     5341 cgccgcggct cacgacctcg ttcgactgca caagcccggc cctcatctcg cggtcgtacc
     5401 gcggtagttg gcggacgagc gtcagcacca tcgccagcgc catctccgcg accggcgcgg
     5461 ccatcggccc gggcgcgttc gccaccgtca ggcgctcgac gacctccggc tccatccgcg
     5521 ccttcagttc gccgccgcag tgcgcgatga gacgcacgcg gcggggctgc gcgaacatct
     5581 ccatccgcag aaacggcgag tgccacgtcg tcatcagcgc gtccgagcga gagagatgct
     5641 cgcgcagctc cgccgagtcc tcgcgcccgt cgtagtgctc ccactcgccc acctcttcga
     5701 gccgcgcgag gacatgcgcg gggaagaagt gcgggaacag cgcgtcgctc gtcagcacga
     5761 gtatgcgcgg gcgagcaagt gtgctcacgc cctcgacttc attcgtctcg gttcgctctt
     5821 cagacatcac taacgctcta tcctctaaca ccacacgcgc actcaggccg cgcccgcctg
     5881 ccctcccgga agggggcgca tcgccgccgc gaagtccggc agagaaacgt ccggctcctc
     5941 tatctccggg tgccagtact tctcgaactc gaagctcacg tagccgcggt agtcgagttt
     6001 gcggagcgcg gccacgaccc gttcgaaggg cacgaggccg cgcccggcga gcacgggcgt
     6061 ccagtgctcc ttgcccatcg ggcgcgcgtc gcgcaggtgt acgtgcgcga ggaagggggc
     6121 gacggctgcg gacgactcct ccacctcttc acccgaagcg aacgtgttgg ccgcgtccca
     6181 gatgatgcag aggttcgggt ggtcggccct gcggatgatg tcggcggtca tgtgcccgcg
     6241 ggcgaagtcg ccgtgcgtct ccagcccgac gcggacgccg cgcggcgcga ggcgtgccac
     6301 ctcacgcagc gagtcgctga tgcggtcgcg cgtctgctca cgcgtagcgc cctcgggcac
     6361 gaggtcgggg aagacgcgga tgagcggggc gccgagcgcc gccgcgagtt cgcagtggcg
     6421 tccggcgagt tcgacctgtc tgcgccgctc cgactcgtcc accgagtcga acgtgcagga
     6481 ggtgtccacg cagcagatgt ccacgccccg tccggcgagc cattcgcgcg tgcgcgcgac
     6541 ctcgccgggt tggaactcgg ggcgggccgg ccaatccagc ccgccgccga gcgcgcgcag
     6601 ttcgacgccc tcgtagccgt actcgcgcgc cgcgcgagcc acctcttcga gttcccagtt
     6661 cgggcagccg agggtcgaga atgccagttt cagcataagc aacgagacga cttcagggcg
     6721 cgtattcgta ggcgccgatg tcgatcagcc cgtccgaggg gcgcgccgca gccgtccccg
     6781 gcgccggcag cgtgtgcagc ggcgggtgga aggcgggcgg ccagaggggg ttgataaagg
     6841 ggtggccggc cggactcgcc ggcgcattat ttccggcgcc gatgagtgcg ctgccgaccg
     6901 ccgggcgcag gtcgagcgtc gtcaggttgg cgaagcccgg gtcggtgccg aagacggtgt
     6961 tcgtccagtc ggtcgggatc atcgccgagc cgaagggcag ccagttgttc gtgcccacgc
     7021 tcacgcgccc cgccacccag cgcacgcgcg tctccagcac gaccttgtca atcggctcgt
     7081 tgcccggacg atagaagacg ttgttgtgga cttcgagact gtcgataccc tcgaagattt
     7141 cgaaggccgg gctgacctgc ccctgccgga ggatgaaggt gttgttgacg aagcggtagc
     7201 gccccttcgt ctgccccgtc ccgtcgctgc cgacgcgcac catgcgcgtc ccctgcgtcg
     7261 ccttgtgctg gaagacgttg ccgaccacgt ccgagtcctc gcgcttcagg tcggtcgaga
     7321 cgttgcggtt gcccttcggg tcggggccga tcagctcgat ctcgccgtag aagccgcccg
     7381 agacccagtt gtagtaaatc tcgttgcgct cggcgcggct cttgatgttg ttgccgccgc
     7441 gctggtcgtg caggtagcag tactgcatgc ggaagaccgc gccggggtac ttgaactcgt
     7501 cggtcgccat gtagacgggg tgctgccccg tgccgttgcc gcagttgtag acctcgacgt
     7561 attcgagcgt gagcgagccg gagtcggtgt cggcgccgag gatgccgtgc gcggggcagt
     7621 cgtggacgac cgtgtcgcgg atggtgatgt cgtgcgcgtg gtggtagatg ccgcggaagg
     7681 agccgcccgt cacgtcgaag ccctcgaaga cgtagtggtt cgagaggcgg aactcgaccg
     7741 tgttcgtccc gcccgaaagc accgggcgct gtccgttgac gcgcaggccg cggatggtga
     7801 tcttgttggt cggcgtgccc ggcacagtga agatgatgcc gccggggtag gtgacgttcc
     7861 cgtccacttc gacgaggtcg ccgggcgcga gcagcggcgc cacgtcctgt agcttcgtgt
     7921 aagtgcgcga ggggccgacc tgataggtgg ccgcctgcac gctgtcggcg gcgccggaca
     7981 gggcgaagca ggcggcgagg aacaagcagg cgacgaagta cctcatctct tatcctccct
     8041 ttcgtctcgg gtcgggattt tcagtggccc cgacgcttgc cacgtgaggg gcgcgggcgg
     8101 gggctgtcgt ccctcggcgc gcgcgcgatg gagcgggcaa gtgcgtaacg aaccgactcg
     8161 aaccggtcgc ccgtctcggc gaagctgacg gccccgccgc gcggcacctc ggagaagacc
     8221 cgcgggacga ccgcccgcac ggcggcctcg acctccgcgt tgaggcccgc cttgcgcgcg
     8281 agcgtcaggt actggtgatc ctgaaggccc cggcggaagt tggcgagctg cacggtcgag
     8341 acggggccgt gcaggccgcg gtcttccgcg gggtgcagct tctcttcgcc ggggtacatg
     8401 agcacgccgt cgccgttgat ccagccctga ctgcccgcgg gcttgcgcgg ctggccgcgg
     8461 ttgtcgaagg tgacggggtt ggcccagacg ttctgctccc gcacgccctg cttctgcgag
     8521 ttgtgccgcc agtggacgcc gtgccagtag aagtagacct cgatgccgtg cttgaagcag
     8581 gcccagatgg tcgcgcgcgg gtcggtggcc ggcgcgtcga tgacgatggc gccggcggcg
     8641 ggacgcccgc cgttataaat ccagtaccgg cggccacgct cgcgctcgcg cctgacctgc
     8701 tcgatgtcga agccctgcgg gccggagtcc cagatgtcta tcgcgccgtc cagctccttc
     8761 gtgtagcgct tggtgacgaa gatggggagc gacttgcccg gccccgggtt cgagtggacg
     8821 ttctcggcga ggcggcggat gtactcgtac tcggacgggc caggctcgtc gggcatgtag
     8881 aggaaggtga gcgccgcggg gaagttctcg cgcaggaagg ccatccactc gtcggagttg
     8941 cgccaggcgc tctcgcgctc gtcgaaatcc ttgccggggc cgtagaagct gcgcgggatg
     9001 acgcggttgc cgaccgactc gccggggcct tcatagccgc gcgcgggcgt gaagtcttcg
     9061 cccgtgaaac gcgcgcgggc ggcgcgcgcc gactcgaccg agtaggcgtg aaccagctcg
     9121 acgcggtggc ggtgcgcgaa gcggtggtac gcggggtcga ggttgcgccc ctgataaagc
     9181 tcgggctgca aaggctcgta gtagaccatc gcgtgcatgc tgttctcgtc cggcagggtg
     9241 aagtcgaaca gctccagctc gacggggagc gtctgcctgc gcccgtcagc cgtcacctcc
     9301 acgcgccctt cgtagacgcc cgcgggcagg tcgcgcgccg tgtagatttc gatccagacg
     9361 gcctggttcc ggcgcgggga gaccttcaag gggaagccgc cccggcccgc cttcgcgttc
     9421 tcggggacga gttggacggg cttccacccc agcgtgtcgg cgggcgcgga ggccgagccg
     9481 gggcgccaga cccagttggc gcgcgtcgtc tcggtcacgt tcatgtagtt gacggagaag
     9541 agctggatgg gtctgccggc gtagtcggtc gggtcggcgg acgggcgcgc gtaacggatg
     9601 ctctcgccgc cgccgcgctt gctgagttcc ggcagtgcga ccgacagcgc gccgatgccg
     9661 tcggcgccgg cctcgacgac gacctgaaag gcgatgacct cgttgcgcgc gccgaagatt
     9721 tttattctcc gcccgtccca ggccgagttc gacgcgcggc tccggttctt caggtcgtct
     9781 ctttcgacct tctcgccgtc gctgacggcc cacacgcgcg cgggctggcc ggcgagcgca
     9841 aggttctgga aaagacagat cagcgatagc agcgtcgcca gctttctcac tttttctccg
     9901 agcctcaagc tatgtcttcc ctccgacgcg cacgcccatc gcgcggcttg cggacgccgc
     9961 gaacgcgggc gcgtctgtaa agtagtccca agccctaatc cttcgccgcc gcttccgcgc
    10021 cgaccgcggc ggccaccctg ccccggacgc cctgcggcgt gcgccccgcg acgacgaagg
    10081 ccagaccgat gacggtggtg acgaccgcgt acgcggcggt gatcaacagc tcccgccccg
    10141 aagtgttggc gagcagcatg agaaagcagg caagcccgat gcccgtcgtc accttcccca
    10201 cgatggccaa ctgcccgcgc cccgacatgc cgctctctcc caactccgtc gcggggctga
    10261 tgggcgtggc cagtttcttg aagaaggcgg agacgcgccc ggcgaactcg cccgtgggct
    10321 tgatgagcag cacggggacg aagaacccga cggtcgtcgt gatcgcgggg acgccgatga
    10381 ggtacgtctc gttcaagccc cagcccagat agcggttgag gtagaagttg acgaagagcg
    10441 aggtggcggc cccgaccgag taggcgaaga cggctgacca ccaggggcct cgcttgacca
    10501 gcatgccgta gaggagcggc agccccagcg gcagcaggaa gatggattcg aggatgaaga
    10561 gcagcgtgaa gatgccggtc gtccccatcg cgaagacgag cgccaccacg atcatcacca
    10621 cgccgaccag cagcgacgcc agccggctcg ctgtcagcat gtgcttctcc gtagcgtcgc
    10681 ggcgcacgta gccctgatag aggtctttgg cgaggatgcc ggtgacgacg tggttgatgg
    10741 ccgagagcga cgccatcgag gaggcgagca tggccgcgag caccacgccg acgagccccg
    10801 gcgggagcag gcgcatcccc atcgagatgt aggcggcctc ggtcggctgc ttcagcccgt
    10861 tggccgcggt cgccagctcc ggcatcacca cgcgcgccac cagcggcggc gtgtagaaga
    10921 cgaacgcgcc cagcgtgaag agcgccatgc agaggacggc gatcctgcgc gcgtcgcgct
    10981 cggtcggcgc gcagaagtag cgctgcgcgt tcccgaagtt gttgtaaccg accgtctgcg
    11041 agatggtgta ggcgacgagg aagagccacc cgtactcgcc cgcgaacggg ttggtgtagc
    11101 cttcgggcgc ctgcgcgacg aagcccgaaa cgcctcccac gtcctgaagc gagaggatca
    11161 tcagcagcag ggcgacgggg aagaggaaca tgaactggaa catgtccgtg aagcagaccg
    11221 cccagtagcc gcccagcacc gtgtaggcga gga
//
LOCUS       DBNGOH010000750         2383 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4510963, whole genome shotgun sequence.
ACCESSION   DBNGOH010000750 DBNGOH010000000
VERSION     DBNGOH010000750.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2383)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2383)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2383
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4510963"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..513)
                     /locus_tag="AC159P_14455"
     CDS             complement(<1..513)
                     /locus_tag="AC159P_14455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3696699.1"
                     /translation="MKPLAGQVAVVAGATRGGGRGIARMLGEAGATVYCTGRSSRAQP
                     NTSDHHYAGRPETIEETAEMVTAAGGRGVAVRVDHTVADEVAALFKRVRREAKRLDIL
                     VNILTGPPVRDWQPFWKLDVGAGREMVERWVWPHVETCRHAVPLMLERKSGLVVEVVE
                     QDSIGYHGQFF"
     gene            complement(585..1109)
                     /locus_tag="AC159P_14460"
     CDS             complement(585..1109)
                     /locus_tag="AC159P_14460"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF013989.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HIR3696700.1"
                     /translation="MRKVKLFVANSLDNFIARPDGGVDWLFTDGDYGMRAFFKSVDTA
                     LLGRKTYDWALAHGGSPDVFKGMAYYVFSRSRRRRGAAPKGVRFVSGDVRSCVETLRR
                     AEGKDIWLMGGGELTESFLRERLIDEIILTVHPVLLGAGLPLFRGVYPQTELKLLKCK
                     TYKTGLVQLSYGLK"
     gene            1222..2196
                     /locus_tag="AC159P_14465"
     CDS             1222..2196
                     /locus_tag="AC159P_14465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003389622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR3696701.1"
                     /translation="MRADRLFSIVLLLQSRRLLTSRELARRLEVSARTIHRDMDALSA
                     AGIPVVAERGTGGGWSLLGEYRTNLTGLSEAEAQSLFVVNPSRLLADLKLEKAAEGAF
                     LKLLAALPGAYRQGAEHARQRIHVDVTGWRRAEEAVPLLHVVQDAVWQERRLRMTYGQ
                     AECGAVERVVDPLGLVAKGSVWYLVAAVEGDVRSYRVSRIAAAELLDYPVARPPDFDL
                     ATHWEESSKRFRAQPPHYTMKARVRPDILPVLPYAGRFARVEPPGRTDAEGWLEVSVG
                     FDAEDLARDYALSFGDRLEVLEPRELREKVVEAARRVVAFYEQKKQGA"
BASE COUNT          387 a          839 c          767 g          390 t
ORIGIN      
        1 gaagaactgc ccgtgatagc cgatgctgtc ctgctcgacg acctcgacga cgaggcccga
       61 cttcctctcc aacatgagcg gcaccgcgtg acggcacgtc tcgacgtgcg gccacaccca
      121 gcgctcgacc atctcgcgcc ccgcgccgac gtcgagcttc cagaacggct gccagtccct
      181 gacgggcggc cccgtcagga tgttcaccag aatgtccaga cgcttcgctt cgcgccggac
      241 gcgcttgaag agcgcggcca cctcgtccgc gaccgtgtgg tcgacacgaa ccgcgacgcc
      301 gcgaccaccc gccgccgtca ccatctccgc cgtctcctcg atggtctccg ggcgaccggc
      361 gtagtggtgg tcgctcgtgt tcggctgcgc gcggctgctg cgtcccgtgc aatagacggt
      421 cgcgcccgcc tcgcccagca tgcgcgcgat gccgcgcccg cccccgcgcg tcgcgcccgc
      481 gaccacggcc acctgccccg ccaatggttt catcgtctcc gacctccctt cgcgttttcc
      541 ttcgtcaaaa aaaacacgcc cgccccgaca ggggcgagcc gccttcactt caacccgtag
      601 ctcaactgca cgagtcccgt cttgtacgtc ttgcacttca gcagcttcag ctcggtctgc
      661 gggtacacgc cgcgaaagag cggcagcccc gcgccgagca ggacggggtg aaccgtcagg
      721 atgatctcgt cgatcaggcg ctctcgcagg aacgactcgg tcagctcgcc gccgcccatc
      781 agccagatgt ctttcccctc ggcgcggcgc agcgtctcga cgcaactccg cacgtcgccc
      841 gagacgaaac gcaccccttt cggcgccgcc ccgcgcctgc ggcgcgaccg cgagaagacg
      901 taataggcca tccccttgaa aacatcgggc gagccgccgt gcgcgagcgc ccagtcgtac
      961 gtcttgcgcc ccaacagcgc cgtgtccacg gacttgaaga acgcacgcat cccgtaatcg
     1021 ccgtccgtga agagccagtc cacgccgccg tcgggccgcg cgataaaatt gtcgaggctg
     1081 ttcgccacga agagcttcac cttccgcatt tgccctctcc ccccttcgac gccttcacga
     1141 tacgcgaaga ttgatgacac cctctgtcat atatccggtt taaaatcacg gtcgggcttt
     1201 ttccttgaag gagatgaaga gatgcgcgcg gatcgtctgt tctccatcgt gttgctgttg
     1261 cagtcgcggc ggctgttgac ctcgcgcgag ctggcgcggc gtctggaagt ctcggcgcgg
     1321 acgattcacc gcgacatgga cgcgctgagc gcggcgggca tcccggtcgt cgccgagcgc
     1381 gggacgggcg gcggctggtc tctcctgggc gaataccgca cgaacctgac gggcctcagc
     1441 gaggccgagg cgcagtcgct cttcgtcgtc aacccttcgc ggctgctcgc cgatttgaag
     1501 ctggagaagg ccgcggaggg cgcgttcctg aagctgctgg cggcgctgcc cggcgcctac
     1561 cggcagggcg cggagcacgc gcgccagcgc atccacgtgg acgtgacggg gtggcgccgc
     1621 gccgaagagg cggtgccgct cctgcacgtc gtgcaggacg cggtgtggca ggagcgcagg
     1681 ctgcggatga cctacgggca ggccgagtgc ggtgccgtgg agcgcgtggt cgatccgctg
     1741 gggctggtgg cgaaagggag cgtgtggtac ttggtcgcgg cggtcgaggg cgatgtgagg
     1801 agctaccgcg tctcgcgcat cgccgccgcg gagttgctgg actacccggt cgcgcgcccg
     1861 cccgacttcg acctcgccac gcactgggag gaatcgtcga agcgtttccg cgcgcagccc
     1921 ccgcactaca cgatgaaggc gcgcgtgcgc ccggacatcc tgcccgtgct cccctacgcc
     1981 ggtcgcttcg cgcgcgtcga gccgccgggc cgcacggacg ccgaaggctg gctcgaagtc
     2041 tccgtcggct tcgacgcgga agacctcgcc cgcgactacg ccttgagctt cggcgacagg
     2101 ctggaggtgc tggagccgcg cgagctgcgc gagaaggtgg tcgaggccgc gcgccgcgtc
     2161 gtcgccttct acgagcagaa gaagcagggt gcgtaaccga accgaccacg aaggcacaaa
     2221 gaagtaggca gtaggcggta ggcagtaaaa gcaggaaggg cacggaggct tgagaggtaa
     2281 agtgccaagc ctccccgctc ttctttctta actgcctacc gcctaccgcc tactgcctac
     2341 tttcttttac tgcttgcgcg agtagacgta gaacatcacg gcg
//
LOCUS       DBNGOH010000751         3739 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2311885, whole genome shotgun sequence.
ACCESSION   DBNGOH010000751 DBNGOH010000000
VERSION     DBNGOH010000751.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3739)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3739)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3739
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2311885"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            385..1404
                     /gene="gmd"
                     /locus_tag="AC159P_14470"
     CDS             385..1404
                     /gene="gmd"
                     /locus_tag="AC159P_14470"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008726057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019673 - GDP-mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HIR3696702.1"
                     /translation="MKKALITGITGQDGSYLAELLLAKGYEVHGIIRRASTFNTGRID
                     HLYQDPHVNGVSLFLHYGDIADSTNLIKLLYRIQPDEIYHLAAQSHVRVSFDIPEYTG
                     DVTGLSTVRILEAVRETGLKSRFYQASSSEMFGKVHEVPQRETTPFHPRSPYGAAKVY
                     AYWATVNYRESYGMFACNGILFNHESPRRGETFVTRKITRAVAHIKAGLQEKLYLGNL
                     DAKRDWGYAKEYVEAMWLMLQQGEPDDYVIATGETHSVGEFLDEAFSYLGLDWRAHVE
                     IDPKYYRPAEVDLLIGDAAKAAERLGWKPRTTFRELVRLMVDADVAALEGAGLKGLQR
                     DVVGR"
     gene            1388..2347
                     /locus_tag="AC159P_14475"
     CDS             1388..2347
                     /locus_tag="AC159P_14475"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase family protein"
                     /protein_id="HIR3696703.1"
                     /translation="MWWEDKRVVVTGGTGFLGSFVVEKLRERGCRQVFVPRSHACDLR
                     SHEAVERLYAETRPHVVIHLAAVVGGIGANRENPGRFFYDNAIMGIQLMEYARRFGVE
                     KFVAIGTICAYPKFTPVPFKEENLWDGYPEETNAPYGLAKKMMLVQAQAYREQYGFNA
                     IYLLPVNLYGPRDNFDLDTSHVIPAMIRKFAEAREAGAAAVSLWGDGSPTREFLYVED
                     AAEGVLLAAERYDGGAPVNLGTGHEISIRDLAQLVAREVGYDGHIAWDASKPNGQPRR
                     CLDVTRAKELFGFRAAHGLREGISKTVAWFMAHRGELREVRVG"
     gene            2566..3486
                     /locus_tag="AC159P_14480"
     CDS             2566..3486
                     /locus_tag="AC159P_14480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3696704.1"
                     /translation="MNSSTGESTGVLGPASTRTVHDAQGPPSRTPPLSEEPLVIIEPS
                     KSWTPLNLRDLWTYRELLYFLVWRDVKVRYKQTAIGVLWVVMQPLLSTLIFTIFLGVL
                     ARVPSDGVPYPVFVFAGVLVWTFFAGAVTSSGNSLVGNSHLITKIYFPRLIIPIAAVG
                     ARLVDFAIAFVILAGMMVYYGVAPTRSLLLLPALVALITLFILGVGMWTSALNVKYRD
                     VGIVLPVLIQLWMFASPVVYPLSLVPEGWRNIYALNPLVGIITGFRAALLGQEFHWTA
                     LALSTVVTLALLVWATYYFRRVERTFADIV"
     gene            3494..>3739
                     /locus_tag="AC159P_14485"
     CDS             3494..>3739
                     /locus_tag="AC159P_14485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015156744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR3696705.1"
                     /translation="MRPIITVEGLSKQYVLGAQQASYRTLRDALAGMARAPLRRLARN
                     GEAGKDLWALRDVNFEVRPGEAVGVVGRNGAGKSTLLK"
BASE COUNT          649 a         1202 c         1244 g          644 t
ORIGIN      
        1 cgcgtctttg cgtcgccgtg aagtgtgcgg tgagggcacg cgcggggcgg cggggaagcg
       61 ggcgggccaa cgtccggcgc gctctcgcaa ggctggcgtc cgcccgattc cgccgcccga
      121 attcgaggcc gcttgacgga ttaccggtgg ctgtctacat taacgtgcgt cggaatgagt
      181 gcccgtcatt agacgaaaga caagacggtt cggagaggga agtcgattcg ccgcccgtga
      241 tctcctcaaa ccggcggggc ggacgccggc ctccttgagg cgccgtcccg cggcccctga
      301 atcattaccg gcaggacagg aataatcatc ccggagcgaa ggaaaatata gtgctgtgat
      361 gaagaaggaa gacttagtgc tgtgatgaag aaggcgctca tcacaggcat cacgggtcag
      421 gacggctctt acctggcgga gctgctgctc gcgaagggct acgaggtgca cggcatcatc
      481 cgccgcgcct cgaccttcaa cacgggccgc atcgaccacc tctaccagga cccgcacgtc
      541 aacggcgtca gcctgttcct gcactacggc gacatcgcgg actcgaccaa tctcatcaag
      601 ctcctctacc gcatccagcc cgacgagatt tatcacctcg ccgcgcagag ccacgtccgc
      661 gtcagcttcg acatccccga gtacacgggc gacgtgacgg ggctgagcac cgtgcggata
      721 ctggaggccg tgcgcgagac ggggctgaag tcgagattct atcaggccag tagctccgag
      781 atgttcggga aggtgcacga ggtcccgcag cgcgagacga cgccctttca cccgcgcagc
      841 ccctacggcg cggctaaggt ctacgcctac tgggcgacgg tcaactaccg cgaatcctac
      901 ggcatgttcg cctgcaacgg gatcctgttc aaccacgaaa gcccgcggcg cggcgagacc
      961 ttcgtaacgc gcaagatcac gcgcgccgtc gcgcacatca aggccggcct tcaggagaag
     1021 ctctacctcg gcaacctcga cgcgaagcgc gactggggct acgccaagga gtatgtcgag
     1081 gcgatgtggc tgatgctcca gcagggcgag ccggacgact acgtgatcgc gacgggcgag
     1141 acgcactcgg tcggcgagtt cctcgacgag gcgttctctt acctcggact cgattggcgg
     1201 gcgcacgtcg aaatcgaccc caagtattac cgcccggcgg aggtcgatct gctgatcggc
     1261 gacgcggcca aggccgcgga gaggttgggc tggaaacccc gtaccacttt ccgcgagctg
     1321 gtgcgcctca tggtggatgc cgacgtggcg gcgctggagg gcgcggggct gaaaggactc
     1381 cagagggatg tggtgggaag ataagcgagt cgtcgtgacg ggcgggacag gatttttagg
     1441 ctcgttcgtc gtcgagaagc tgcgggagcg gggctgccgc caggtcttcg tcccgcgctc
     1501 gcatgcctgc gacctgcgca gccacgaggc cgtcgagcgc ctgtacgccg agacgcgacc
     1561 gcacgtcgtc atccacttgg cggcggtcgt cggcggcatc ggcgccaacc gcgagaaccc
     1621 gggccgcttc ttctacgaca acgccatcat gggcatccag ttgatggagt acgcgaggcg
     1681 gttcggggtc gaaaagttcg tcgccatcgg caccatctgc gcctacccca agttcacccc
     1741 ggtcccgttc aaagaggaga acctgtggga cggctaccca gaggagacga acgcgcccta
     1801 cggcctcgcc aaaaagatga tgctcgtgca ggcgcaggcc taccgcgagc agtacgggtt
     1861 caacgccatc tacctcctgc cggtcaacct ctacgggccg agggataatt tcgacctcga
     1921 cacgtcgcac gtcatccccg ccatgatccg caaattcgcc gaagcgcgcg aggccggcgc
     1981 cgctgcggtc tcgctgtggg gcgacggctc gcccacgcga gagttcctgt acgtcgagga
     2041 cgcggcggag ggcgtgctgc tcgcggcgga acgttacgac ggcggcgcgc cggtcaacct
     2101 cggcaccggg catgaaatct cgatccgcga tctggcgcag cttgtggcgc gagaggtggg
     2161 ctacgacggc cacatcgctt gggacgcttc caagccgaac ggccagccgc ggcggtgtct
     2221 ggacgtgacg cgcgccaaag aactcttcgg cttccgcgcg gcgcacggct tgcgcgaagg
     2281 catctcgaag acggtcgcat ggttcatggc ccaccgcggc gagttgcgcg aggtcagggt
     2341 tggctgaggc cggagagttt gacaggggct tgataaaact ctcacccgtg gccctgagaa
     2401 gtccgcgggc ggcccgccgg aaggccaggt tgacggtcgc taagggcgcc gttacactga
     2461 cacctcccgc acaacttgaa gggctgtctt aaagtgccgg ctcctcgccg cgggcgcgga
     2521 cgcggcgggt gtttgcacag acaagcttgg tgaaggtcgg ttttgatgaa ttcaagcact
     2581 ggcgaaagca cgggcgtact ggggccggct tcgacgcgaa ccgttcatga cgcccagggg
     2641 ccgccctccc gcacgccccc gctgtcggaa gagccactgg tcatcatcga gccgagcaag
     2701 tcgtggacgc cgctcaacct ccgggacttg tggacgtacc gcgagctgct ctactttctc
     2761 gtctggcgtg atgtcaaggt ccgctacaag cagacggcca tcggcgtgct gtgggtcgtg
     2821 atgcagccgc tcctgagcac cctcatcttc acgatctttc tgggggttct ggcgcgcgtc
     2881 ccttcggacg gcgtcccgta cccggtcttc gtcttcgccg gggtgctggt ctggacattc
     2941 ttcgcggggg ccgtgacgag cagcgggaac agcctcgtcg ggaacagcca cctcatcacc
     3001 aagatttact tcccgcgcct catcatcccg atagcagcgg tgggggcgcg gctggtcgat
     3061 ttcgccatcg cgttcgtcat cctcgccggg atgatggtct attacggcgt cgcgccgacg
     3121 cgcagcctcc tgctgctgcc tgccctcgtc gccctgatca cgctgttcat cctcggcgtg
     3181 gggatgtgga cctcggccct gaacgtgaag tacagggatg tcggtatagt gctgccggtg
     3241 ctgattcagt tgtggatgtt cgcctcgccc gtcgtgtacc cgctgagtct cgtgccggaa
     3301 ggctggcgga atatttacgc gctgaacccg ctggtcggca tcatcacggg cttccgcgcc
     3361 gcgctgctcg gccaggagtt ccactggacg gcgctggccc tgtccaccgt cgtcaccctc
     3421 gccctgctcg tctgggccac ttactacttc cggcgggtgg agaggacttt cgccgatatc
     3481 gtttagccga gcgatgcgac cgatcatcac agtcgagggg ctgagcaaac agtacgtgct
     3541 cggcgcgcag caggcgtcgt accgcacgct gcgcgacgcg ctggccggga tggcccgcgc
     3601 ccctctgcgc cgcctcgcgc gcaacggcga ggccgggaaa gacctctggg cgctgcgcga
     3661 cgtgaacttc gaagtccgtc cgggcgaggc ggtcggcgtc gtcgggcgca acggggccgg
     3721 caagagcacg ctcctgaaa
//
LOCUS       DBNGOH010000752         5288 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1946045, whole genome shotgun sequence.
ACCESSION   DBNGOH010000752 DBNGOH010000000
VERSION     DBNGOH010000752.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5288)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5288)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5288
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1946045"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..420
                     /locus_tag="AC159P_14490"
     CDS             <1..420
                     /locus_tag="AC159P_14490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696706.1"
                     /translation="HAFESQLDAHCRHPQGVIDQSILLWNMYYTVARGFRAAHPSWLF
                     VKHEELSADPVAQFRELYRQFNLSWSEKVKEGIEEYTRPGNLKEDSPREVWSVKRDSR
                     ATRNLWAERLTPEEIARIREGVAEVAQGYYTEADWQP"
     gene            complement(538..1662)
                     /locus_tag="AC159P_14495"
     CDS             complement(538..1662)
                     /locus_tag="AC159P_14495"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR3696707.1"
                     /translation="MRVLLTNHDLATRGGTQVYVRDLATELLRRGHTPIVYSLTHGDV
                     ARELRSRTVPVVSDLDEVPPPDLIHGQEHLGTMTALLRYPRAPAIFFCHSFFGWGSAP
                     PRFPRIVRYVAVDEACRDRLVLEHGIPAERVRTLLNYVDLGRFRARDPLPSAPRRAVL
                     FSNYRMKPPEVEVLRGVCAETGLTLDVIGEAGGAAASSPEELLGGYDLVFAKGRSALE
                     ALAVGAAVVVCGVERMGPLVTAAEFERLRDLNFGVRTLHEPISAAALRREIRRYDARD
                     AAEVARRTRLAAGAAAAFDETIELYEEVIAEHASAPCDADEEARAAASYLRRLQVELD
                     THTAASIRIQKRLLKVPVIGKPVHRLLRRVAGRVRPRSEF"
     gene            complement(1691..2548)
                     /locus_tag="AC159P_14500"
     CDS             complement(1691..2548)
                     /locus_tag="AC159P_14500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696708.1"
                     /translation="MHTGEPFEASARAALVTAHPGHELCVLGWLKRARPRVFIITDGS
                     GRGGEPKLEATTDLLARAGASRGSFYGRWTDAHFYRAMLCGDTDFFVRLAAEFADIFA
                     RERFDYVACEAIEGYNPTHDLCRLLTFAALARANRAGGHRTAGYEFALIDRGRARHET
                     PRADSIWLRLDDDALGEKLAEMRAHPHLADEVGAGLDGSALAALRDFPEIAAEVQALV
                     RGMGPEAFRVEHLRAARPGVDEPTPVAPFYERYGQCLVRRGHYAQAIGFSRHVAPIAE
                     RLRRFAEGD"
     gene            complement(2862..3314)
                     /locus_tag="AC159P_14505"
     CDS             complement(2862..3314)
                     /locus_tag="AC159P_14505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696709.1"
                     /translation="MRSNGLLLKGASALALGLAMLVPMVSVSAQDWRTQQRRENRRER
                     REERREERQERREERREARRGRDWDGYGNYGGSSQLRQTALNAGYNEGIEEGRNDRRR
                     GERFNFADEGDYRNASKDYNSRFGNRALYQRYFRAGFENGYRDGWNGY"
     gene            complement(3578..4120)
                     /locus_tag="AC159P_14510"
     CDS             complement(3578..4120)
                     /locus_tag="AC159P_14510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696710.1"
                     /translation="MGAFGQWLMHEDQRDVWDSIYASALAVIFFALSALLLWPLGRLM
                     MVWSLFKGYWLFCTVLGVTALLLFLFRRVLRIDLETRYDAYVLSAFAVSGFLLAGWSA
                     FAALAVRGHVEGASGWVAAALFVIGFLSCYVAFAVACVYYTGQMYRLVNLPLAWLSFI
                     IFSVWPAAARVVYGWFFDLF"
     gene            complement(4342..>5288)
                     /locus_tag="AC159P_14515"
     CDS             complement(4342..>5288)
                     /locus_tag="AC159P_14515"
                     /inference="COORDINATES: protein motif:HMM:NF015863.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HIR3696711.1"
                     /translation="AEIGYGREGVVFVNPYTGEVLGEGSREVRSFFRVVTNWHRWLGA
                     SGEGQSRTVGRAITGACNLAFLFLVVSGFYLWWPRTWTRKSLRSVTWFKRGLEGRARN
                     FNWHNTAGFWTAVPLFVVVLSAVVISYTWASNLVYRLMGETPPAPARGAGQPAGNAAP
                     QRGGAERRGEDAAAAQESADVDLLWSRAEQQMHGWRSISLRLPSPADSTVTFTLDQGD
                     GGQPQKRAQLTLDRRTGEVVRWEPFSSLTPGRRLRSLLRFAHTGEVAGGVGQAVAALA
                     SAAGALLVWSGLALALKRFRNWLARRSGGASGPDPIPY"
BASE COUNT          944 a         1802 c         1755 g          787 t
ORIGIN      
        1 cacgcgttcg agtcgcagct cgacgcacac tgccgacacc cgcagggtgt catcgaccaa
       61 tccatactgc tctggaacat gtactacacc gtcgcgcgag gattccgcgc ggcacaccct
      121 tcgtggctct tcgtcaaaca cgaagaactg tcggctgacc cggtcgcaca gttccgcgag
      181 ctttaccggc aattcaatct cagttggagc gagaaggtga aagagggcat cgaggaatac
      241 acgcggcccg gcaacctcaa agaggactcg ccgcgagagg tctggtcggt gaagcgcgac
      301 agccgcgcga cgcgaaacct ttgggccgaa cgcctcacgc ccgaagagat cgcgcgcatc
      361 cgcgagggcg tcgccgaagt cgcgcaaggc tactacacgg aagccgactg gcaaccataa
      421 gaaggggcga aggggcgaag gggcgaagag gggaagatgg gaaaagggag aagggcgaag
      481 agggttttgg cttctccttt tcgcctcttc cccttttccc ctcttcgccc ttggttttta
      541 gaactcgctg cgcgggcgga cgcgaccggc gacgcggcgc aggagtctgt ggacgggctt
      601 gccgatgacg gggactttga gcagtcgctt ctgtatgcgg atcgaggcgg cagtgtgtgt
      661 gtcgagttcg acttgcaggc ggcgcaggta gctggccgcc gcgcgggctt cctcgtccgc
      721 atcgcacggc gccgacgcgt gctcggcgat aacctcctcg taaagctcga tggtttcgtc
      781 gaacgccgcc gccgcccccg cggcgagccg cgtgcgtcgc gcgacctccg cggcgtcgcg
      841 cgcgtcgtag cgacgtatct cgcgccgcag cgcggcggcg ctgatcggct cgtgcagcgt
      901 gcggacgccg aagttgaggt cgcggagccg ttcgaactcc gccgccgtga cgagcggccc
      961 catccgctcg acgccgcaga cgacgaccgc cgccccgacc gcgagcgctt ccagcgccga
     1021 acgccccttc gcgaagacga ggtcgtaacc gccgagcaat tcttcgggcg agctggccgc
     1081 ggcgccgccc gcctcgccga tcacgtcgag cgtcagcccc gtctccgcgc agacgccgcg
     1141 cagcacctcg acctcgggcg gcttcatcct gtagttgctg aagaggacgg cgcggcgcgg
     1201 cgcagagggc agcgggtcgc gcgcgcggaa gcgaccgaga tcgacgtagt tgaggagcgt
     1261 gcgcacccgc tccgcgggga tgccgtgttc gaggacgagg cgatcacgac aggcttcgtc
     1321 cacggcgaca tagcgcacga tacgagggaa gcgcggcggg gcgctgcccc aaccgaagaa
     1381 gctgtgacag aagaagatgg cgggcgcgcg tgggtagcgc agcagcgccg tcatcgtgcc
     1441 gaggtgttcc tgcccgtgaa tgaggtcggg cggcggcact tcgtcgaggt cgctgacgac
     1501 gggcacggtg cgcgaacgca gctcccgcgc gacatcgccg tgcgtgagac tgtagacgat
     1561 tggcgtgtgc ccgcgccgga gcagctccgt ggcgaggtcg cgcacgtaga cctgcgtgcc
     1621 gccgcgtgtc gcgaggtcgt ggttggtgag cagaacgcgc aaatgctaat cacccggcgg
     1681 cggagcgccg tcaatcacct tcggcgaagc gccgcagccg ctccgcgatg ggcgcgacgt
     1741 gacggctgaa gccgatggcc tgcgcgtagt ggccgcgccg gacgaggcac tggccgtaac
     1801 gctcgtagaa gggcgccacc ggagtcggct catcgactcc gggcctagcg gcgcgcaggt
     1861 gttcgacgcg gaaggcttcc ggccccatcc cgcgcacgag cgcttgaacc tcggcggcga
     1921 tctcgggaaa gtcgcggagc gccgccagcg cgctgccgtc aagccccgcg ccgacctcgt
     1981 ccgcgaggtg cgggtgcgcg cgcatctccg ccagcttctc gccgagcgcg tcgtcgtcga
     2041 gccgcagcca gatcgagtcg gcgcgcggcg tctcgtgccg cgcgcgcccg cggtcgatga
     2101 gcgcgaactc gtagccggcg gtgcggtgtc cgcccgcgcg gttcgcgcgc gcgagcgcgg
     2161 cgaaggtcag caggcggcag aggtcgtgcg tcgggttgta gccttcgatg gcctcgcagg
     2221 cgacgtagtc gaatcgctcg cgcgcgaaga tgtcggcgaa ctcggcggcg aggcggacga
     2281 agaagtccgt gtccccgcag agcatcgcgc ggtagaagtg cgcgtccgtc cagcgcccgt
     2341 agaagctccc gcgcgatgcg cccgcgcgcg cgagcaggtc ggtcgtcgcc tcaagcttcg
     2401 gctcgccgcc ccgccccgac ccgtccgtga tgatgaacac gcgcggtcgc gccctcttca
     2461 gccagccgag cacgcagagt tcgtgacccg gatgcgccgt gacgagcgcc gcccgcgcgc
     2521 ttgcctcgaa tggttcccct gtgtgcatgg ttcttttcgg gcgggcggcg aaaccacggc
     2581 cccgtgcgtc gctcggttga agtcgagacg agcgagggct gacgcaggca aacaagccgc
     2641 accgccggat gaaggatcgc aggattcaga agcctaaagg ttccgtcgag ccaagaggta
     2701 gctttatgct ggatggagtt tggtttgtca agaaagggtc agcgcgggcg tcacgggcgc
     2761 cgacaacaaa cggcgggccg gaagtctcca cttatcaaaa aggaaaggcg tcaggctccc
     2821 cctgagcctg acgcctctcg tccgtgcagc cgtcgcggtt cttagtagcc gttccagccg
     2881 tcgcggtagc cgttctcgaa gccggcgcgg aagtagcgct ggtagagcgc gcggttgccg
     2941 aagcgcgagt tgtagtcctt cgacgcgttg cgatagtcgc cctcgtccgc gaagttgaag
     3001 cgctcgccgc ggcggcggtc gttgcgcccc tcttcgatgc cctcgttgta gcccgcgttg
     3061 agcgcggtct gacggagttg cgaagagccg ccgtagttgc cgtagccgtc ccagtcgcgt
     3121 ccgcgccggg cctcgcggcg ctcctcgcgc ctctcctgcc gctcttcgcg gcgctcctcg
     3181 cggcgctcgc ggcgattctc acgccgctgc tgagtccgcc agtcctgcgc cgagacgctc
     3241 accatcggca ccaacatcgc cagaccgagc gccagcgccg acgcaccctt caataacaaa
     3301 ccattgcttc tcatcttgta tgtttccccc taaaatttcg acgcttcacg gcgacaccat
     3361 gtcggcggag cgtttcactc gcgcttaact actacaagcc ccgtgccacg ggcgcgtttc
     3421 ggccacaatg cctattaaac cccatctaat gcttataaag atgcggcctg ttgtgccgcc
     3481 ggcattgcgc cctacacgat aaggtgtccg ttctcaccac aacgtgagcg cgacggtgta
     3541 cgccgatcgt cccgtttacg aaactttaca tggcctctca aaacaggtcg aagaaccatc
     3601 cgtagacgac gcgcgccgcg gcgggccaca cgctgaagat gatgaacgag agccacgcga
     3661 gcggcaggtt gaccagtctg tacatctgcc ccgtgtagta cacgcacgcg acggcgaagg
     3721 cgacgtaaca agacagaaac ccgatgacga acagcgccgc cgcgacccac ccggacgcgc
     3781 cctcgacatg accgcggacg gcgagcgccg cgaaggcaga ccagcccgcc agcagaaagc
     3841 cgctgacggc gaacgccgaa agcacgtagg cgtcgtaacg ggtctcaagg tcgatgcgga
     3901 ggacacgccg gaagaggaac agcagcagcg cggtcacgcc gaggacggtg cagaagagcc
     3961 agtaaccctt gaacaggctc cacaccatca tcaacctgcc cagcggccag agcagcagcg
     4021 ccgagagcgc gaagaagatg acggcgaggg cggacgcgta gatggagtcc cacacgtccc
     4081 tctggtcttc gtgcatcagc cactgaccga acgctcccat caagtcactc tcctgcctcg
     4141 caagccatga agcgagggct gcgcggcggg ctgctcggtg ctacagccct gaccgcacag
     4201 gctcaggcag aagaccaaca aggctggacg agcacgcaac atctgctttc atcttcgcat
     4261 actccgtccg ccacgtcagg tgagcgcccc gaagacgcac agcaccacgc gcgccgccgc
     4321 aggaacaact gtagctctca gttaataagg gatcgggtcg gggccgctcg ctccgccgga
     4381 acgcctcgcc agccagttgc ggaagcgctt cagggcgagc gccagcccgg accagacgag
     4441 taatgcgcca gccgccgacg cgagcgcggc gacggcctgc ccgacgccgc cggccacctc
     4501 gcctgtgtgc gcgaaccgca acagcgaacg gagccgccgc cccggcgtga ggcttgagaa
     4561 cggctcccag cgcacgacct cgcccgtcct cctgtccagc gtgagttgcg cgcgcttttg
     4621 gggctggccg ccatcgccct ggtcgagcgt gaacgtcacg gtcgagtccg cgggtgaagg
     4681 cagacgcaga ctgatgctcc gccagccgtg catctgttgc tcggcgcgag accacagaag
     4741 atcaacgtcg gcggactcct gcgcggctgc cgcgtcttca ccacggcgct ccgctccgcc
     4801 gcgttgaggc gcggcattcc ctgcgggctg tcccgcgcca cgcgcaggcg cgggcggcgt
     4861 ctcgcccatc aagcgataga cgaggttgct cgcccacgtg taagagataa cgaccgcgga
     4921 gagtacgacg acgaagagcg gcacggcagt ccagaagccc gccgtgttat gccagttgaa
     4981 attccgcgcc ctcccttcaa gcccgcgctt gaaccacgtc acgctccgca gggacttgcg
     5041 cgtccaagtg cgcggccacc acaggtaaaa gccgctgacc acgaggaaca ggaaggcgag
     5101 gttgcacgcg cccgtaatcg cacgcccgac ggtgcggctc tgcccctcgc ccgacgcgcc
     5161 gagccagcgg tgccagttcg tcaccacgcg gaagaacgaa cgtacctccc gcgacccctc
     5221 gccgaggact tcgcccgtgt aagggttgac gaagacgacc ccctcgcgcc cgtagccgat
     5281 ctccgcag
//
LOCUS       DBNGOH010000753         3874 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4694609, whole genome shotgun sequence.
ACCESSION   DBNGOH010000753 DBNGOH010000000
VERSION     DBNGOH010000753.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3874)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3874)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3874
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4694609"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            336..920
                     /locus_tag="AC159P_14520"
     CDS             336..920
                     /locus_tag="AC159P_14520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR3696712.1"
                     /translation="MQKPSSEEITGLLIAWGGGDRTALDKLVPLVYQELRRLARRQMR
                     RERAGDTLQTTALVNEAYLRLVDYERVQARDRTHFLALAAQAMRRILIERARSRRSGK
                     RGSNPQRLSLEDVGEVADERAAVLLALDDALRALAAIDPRKARLVELRYFGGLTIEET
                     AEVLGISTPTVERGWRTARIWLHREISKAVTSDG"
     gene            971..3619
                     /locus_tag="AC159P_14525"
     CDS             971..3619
                     /locus_tag="AC159P_14525"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR3696713.1"
                     /translation="MTPERWQRIEQLLEAALERGPAERAALLDRECAGDPGLREEVES
                     LLASAQPAESILASNALEDAAALLGDSESGSLVGRSVGPYSIEKQIGSGGMGEVYLAE
                     DVRLGRKVALKLLDPGLAGDPQSRGRFLREARLASALDHPNICTVHEVGQARGRPFIA
                     MQYVEGETLRPLVGGRPLKLDSLLSIALQVADALSAAHERGVIHRDIKAGNIIVTPQG
                     QAKVLDFGLAKLLERAEGEAETHLTMTGQVMGTPASMSPEQARGERADHRSDIFSFGC
                     VLYEMATGQIPFKGKTRADVIGALLHKPHTPAVELNGEIPARLSAAIDRALAKEPADR
                     YQTVREMIADLRQVVAEAGGLDHLFSSSGVPRGVLPLVPTRRRGLFGVLGRGAHSRRA
                     AVALVVGILLFAGLALAIFFSRPARPRPSAPLKSIAVLPFKPLVAEGRDEALELGMAD
                     TLIARLSSIREIDVRPVSAVHKYTGLGQDALAAGREQRVDAVLDGHIQKAGEKVRVTV
                     RLLRVGDGAQLWADKFDAEMTDIFAVQDSIAERVTAALAVRLAGADRERLTKHHTENA
                     EAYQLYLKGRYHLNRLTDDGFRRGRDYFQQAIDLDPTYALAYAGLADAYQMLSGYNAL
                     APNEGFPKAKAAATEALRLDEGLAEAHTALGTVKFLHDWDFEGAEKDYRRAVEINQSY
                     SDAHLMYGLYLSAMERFDEAHAEVRRARELDPLSIAKIAGIGDVFYQQRQYDRAIEHY
                     RQALEMDPNSGFAHWALGNAYVNKGVHEEAIAEYRKAIPLSGDSPDELASLGYAYALS
                     GRRREAQAVIAELQERSRRRYISPTIIAFVFAGLGENDQAFAWLEKAFDGRDFILVFL
                     KVDPTFDRLRTDPRFADLMRRVGLPP"
BASE COUNT          669 a         1275 c         1288 g          642 t
ORIGIN      
        1 ccctccgggc cgaagttcag accgcgcggg ttgttcaagc cggatgcgac cagctctgtc
       61 gtctcagggc ttgcggcggc cgccgtcctg acgcggtcgc cgcagatgag ggctgccgtc
      121 gccgcgccga agagtgcagc gagggtgagc gagaccgaga ttctgtttct ggtattcata
      181 actttcctcc cgtcagtagt tttgtcggag caccgtcagt tgcctccaac tggttcggcg
      241 aaaacagaat cgaaatccca tcacgcgccc aggattattt accgccgcga gggtgctata
      301 atccgcgccg ctcccgacca actctgccga atccgatgca gaagccttcc tccgaagaga
      361 tcaccggcct gctcatcgcg tggggcggcg gcgaccgtac ggcgctcgac aaactggtgc
      421 cgctggtcta tcaggagctg cggcggctgg cccgtcggca gatgcggcgc gagcgagccg
      481 gcgacacgct ccagacgacg gcgctcgtca acgaggcgta cctccggctc gtggactacg
      541 agcgcgtgca ggcgcgcgac cgcacgcact tcctcgccct cgccgcgcag gccatgcgcc
      601 gcatcctcat cgagcgcgcg cgcagccgtc gctccggtaa gcgcgggagc aatccgcaga
      661 ggctctctct ggaagacgtg ggcgaagtgg ccgacgagcg cgccgcggtc ctcctcgcgc
      721 tcgacgacgc gctccgcgcc ctcgcggcga ttgacccgcg caaagctcgc ctcgtcgaac
      781 tacgttactt cggcgggctg accatcgaag agaccgccga ggtgctcggc atctcgacgc
      841 cgacggtcga gcgcggctgg cgcacggccc ggatttggtt gcacagggaa atctcgaagg
      901 cggtgacgag tgacgggtga caagttgaaa acttgccctt gctcgtcacc cgtcactcgt
      961 cactgctgtt atgactcccg aacgctggca acggatcgag cagttgcttg aggctgcgct
     1021 cgaacgcggg ccggcggagc gcgccgccct gctcgacagg gagtgcgcgg gcgatcccgg
     1081 cttgcgcgag gaggtggagt cgctgctcgc gtcggcgcag cccgcggaga gcattctcgc
     1141 gtcgaatgcc cttgaggacg cggccgcgct actcggtgac tcggaatcag gctcgctcgt
     1201 cgggcgcagc gtcggtccgt attccatcga gaagcagatc gggtcgggcg ggatgggcga
     1261 ggtctatctc gccgaggacg tgaggctggg ccgcaaggtc gcgctgaagc tgctcgaccc
     1321 cggactcgcg ggtgaccccc agtcgcgggg gcgcttcctc cgggaagcgc ggctcgcctc
     1381 cgcgctcgac catccgaaca tctgcaccgt ccacgaggtc ggccaggcgc ggggccgccc
     1441 cttcatcgcc atgcagtacg tcgagggcga gacgctgcgg ccgctcgtcg gcgggcgtcc
     1501 gctcaagctc gacagcctgc tctcgatcgc gctgcaggtg gccgacgcgc tctcggcagc
     1561 gcacgaacgg ggcgtcatcc accgcgacat caaggccggc aacatcatcg tcacgccgca
     1621 gggtcaggcg aaggtgctcg acttcggctt ggcgaagctg ctcgagcgcg ccgagggcga
     1681 ggccgaaacc cacctgacca tgacggggca ggtgatgggc acgcccgcct ccatgtcgcc
     1741 ggagcaggcg cgcggtgagc gtgcggatca ccgcagcgac atcttctcgt tcggctgcgt
     1801 cctctacgag atggcaacgg ggcagattcc cttcaagggt aagacgcgcg ccgacgtgat
     1861 cggcgcgctg ctccacaagc cccacacgcc cgccgtggaa ctgaacggag agatacccgc
     1921 gcggctgtcg gcagcgatcg accgcgcgtt agctaaagag ccggcggacc gctaccagac
     1981 ggtgcgggag atgattgccg atctgcgtca ggtcgtcgcg gaggcggggg ggctggatca
     2041 cctcttcagc tcgtcgggag tgccccgcgg cgtgttgccg ctggtcccga cgagacgccg
     2101 ggggctgttc ggggtgttgg gccgcggggc tcacagtcgc agggcggcgg tcgcgttggt
     2161 cgttggtata ctgttattcg ccggccttgc gctcgcaatc ttcttctcgc gccccgcgcg
     2221 gccgcgacca tccgcgccgc tcaagtccat cgcggtgctg cccttcaagc cgctcgtggc
     2281 ggaaggccgc gacgaggctt tagagctggg catggccgac actttgatcg cgcgcctcag
     2341 cagcatacgt gagattgacg tgcgccccgt cagcgccgtc cataagtaca cggggctggg
     2401 gcaggatgcg ctcgccgccg ggcgagagca gcgtgtggac gcggtgctcg atggacacat
     2461 ccagaaggcg ggcgagaagg tcagggtcac ggtgcggctg ctcagggtcg gggacggcgc
     2521 gcaactctgg gccgacaagt tcgacgcgga gatgactgac atcttcgccg tgcaggattc
     2581 gatcgcggag cgtgtgacag cggcgctggc cgtccggctc gcgggcgccg atagggagcg
     2641 gctcaccaaa caccacaccg aaaacgccga agcttaccag ctctacctga aagggcgtta
     2701 ccacctgaac aggctgacgg acgacggctt ccggagaggc cgcgactact tccagcaggc
     2761 gatagacctc gaccccactt acgcgctcgc ctacgccggg ctggccgacg cctaccagat
     2821 gctcagcggc tacaacgcgc tcgcgccgaa cgaaggcttc cccaaagcaa aggcggcggc
     2881 gacggaggcg ctccggcttg acgaagggct ggccgaagcg cacacggcgc tcgggaccgt
     2941 caaattccta cacgactggg actttgaggg tgccgagaag gactaccgac gcgccgtcga
     3001 gatcaaccag agctattcgg atgcccacct catgtacggc ctctacttgt cggccatgga
     3061 gaggttcgac gaagcccacg ccgaggtcag acgcgcgcgg gagctcgacc cgctctcgat
     3121 cgcgaagatc gcgggcatcg gagacgtttt ttaccagcaa cgccaatacg accgtgcgat
     3181 tgagcattac cggcaggcgc tcgagatgga cccgaactcc ggcttcgccc actgggcgct
     3241 cgggaatgcc tacgtgaaca aaggggtaca cgaggaagcc atcgcggaat atcggaaggc
     3301 aatacccctt tcgggcgaca gccccgatga gttggcttcg ctcggatatg cctacgcgct
     3361 gtcgggcagg aggcgcgagg cgcaggccgt gatcgccgag ttgcaggaac gctcaaggcg
     3421 acgctacatc tcgccgacca tcatcgcgtt cgtcttcgcc ggactcgggg agaatgacca
     3481 ggccttcgcg tggctggaga aggctttcga cgggcgggac ttcatccttg tcttcctcaa
     3541 ggtcgatccg accttcgacc gtcttcgcac agaccctcga ttcgcagatc tcatgcggcg
     3601 cgtcggcctt ccgccttagc cgaagacttt tgccatacgc acgttagccg agaggcctcc
     3661 acccggccgt tcgataactc cccggaaggg tttcccgtac tttcgatact gcctgtgaag
     3721 tcgattgacg ccgaattccg gctcgtaata tggggaagcg ttcaatgatt cttcccccgt
     3781 ttagtagaca ccgagataag actgccttcg ggcctcacgc cgcctctttc aactgcgccc
     3841 ggtagcgcgc ctcgtactcc gccggcgcga agta
//
LOCUS       DBNGOH010000754         1801 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3229369, whole genome shotgun sequence.
ACCESSION   DBNGOH010000754 DBNGOH010000000
VERSION     DBNGOH010000754.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1801)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1801)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1801
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3229369"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            131..1117
                     /locus_tag="AC159P_14530"
     CDS             131..1117
                     /locus_tag="AC159P_14530"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018904844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HIR3696714.1"
                     /translation="MRPFKYTRAVDAAGATRAVAANPQAKFLAGGTNILDLMKEDVER
                     PNEIIDLTRLKLSEIKAAGGGVSIGALAKNTDTANHQLIRKNYPLLTQAIVAGASGQL
                     RNMATNGGNLLQRTRCQYFYDTAMPCNKREPGTGCGALEGLNRIHAVFGWSEKCVATY
                     PGDMANALYALDAVVRVRGTDGRERTIPVGEFHRLPGDTPEKDNNLAHGELITAIELP
                     KSSFAKNSYYLKVRDRASYAFALVAVAAALELDGGTIKQARLVLGGVAHKPWRSPEAE
                     AALVGKSASEETFRRAGEAALAGAKPLAHNAYKVELGKRAVVRALMRASRLV"
     gene            1136..>1801
                     /locus_tag="AC159P_14535"
     CDS             1136..>1801
                     /locus_tag="AC159P_14535"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016863646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HIR3696715.1"
                     /translation="MARYIGKEMSRVDGVAKVTGRAKYAAEFQAPNLAYGFIVLGTTA
                     KGTIRTIDTREAELAPGVVRVFTHLNAPKLGPKASTEDSPPRATEERDKSFRALQSDR
                     IYFNMQPVALVVAETYEQARYAARLVKVSYNAEKHQTDTESVRDRARAPSQGPPPKPR
                     GNPDAALRSAPVKVEAEYRIAVEHHNPMEPHAAVAAWEGDKLTVFDKTQEVYSVRTHL
                     ASSF"
BASE COUNT          345 a          594 c          624 g          238 t
ORIGIN      
        1 atctgccgct gcggcgccta cccggggatc gtcgccgcca tccaggaggt tcattcgggc
       61 aaagagacgg cgcggacgtg gcgcttcgtc gaagagcaag agctggcgcg ggtcaggaag
      121 gagtggaccg atgagaccgt ttaagtacac acgcgccgtg gacgcggcgg gggccacccg
      181 cgcagtcgcg gcgaacccgc aggcgaagtt ccttgccgga ggaaccaaca tcctcgattt
      241 gatgaaagag gacgtcgagc gcccgaacga gatcatagac ttgacccgct tgaagctctc
      301 ggagatcaag gccgcgggcg gcggcgtctc catcggcgcg ctggcgaaaa acaccgacac
      361 cgccaaccac cagctaatcc gcaagaatta cccgctgctg acgcaggcca tcgtcgcggg
      421 cgcgtcgggg cagttgcgca acatggcgac caacggcggg aacctgttgc agcgcacgcg
      481 ctgccagtac ttctacgaca ccgcgatgcc ctgcaataag cgcgagccgg gcacgggctg
      541 cggcgcactc gaagggttga accgcatcca cgccgtcttc gggtggagcg agaagtgcgt
      601 ggcgacttac cccggcgaca tggcgaacgc gctctacgcg ctcgacgccg tcgtgcgtgt
      661 caggggcacg gacgggcgcg agcgcacgat tcccgtcggc gagttccatc gcctgcccgg
      721 cgacacgccg gagaaggata acaacctcgc gcacggcgaa ctgatcacgg ccatcgagct
      781 gccgaagtcg agcttcgcga agaactccta ctacctgaag gtgcgcgacc gcgcctctta
      841 cgccttcgcg ctcgtcgcgg tggccgcggc gctcgaactc gacggcggga cgattaagca
      901 agcgcgtctc gtgctggggg gcgtggcgca caagccctgg cggagtccgg aggccgaggc
      961 cgcgctggtc ggcaagtccg cgtcggagga gaccttccgg cgtgcgggcg aggcggcgct
     1021 cgcgggcgcg aagccgctgg cgcacaacgc ctacaaagtc gaattgggga agcgcgcggt
     1081 cgtccgcgct ctgatgcgcg cgagcaggct tgtctgacgg gacggaggcg acgagatggc
     1141 aaggtacatc gggaaagaga tgagccgcgt cgacggcgtg gcgaaggtga cgggcagggc
     1201 gaagtacgcg gccgagttcc aggcgccgaa cctcgcgtat gggttcatcg tgctggggac
     1261 gacggctaaa ggcacgatca ggaccatcga cacgcgcgag gccgagctcg cgccgggcgt
     1321 cgtccgcgtc ttcacgcatc tgaacgcgcc gaagctcggg ccgaaggctt ctacggagga
     1381 ctcaccgccg cgcgccaccg aagaacgcga caagtctttc cgcgcgctcc aatcggacag
     1441 aatctacttc aacatgcagc ccgtcgcgct cgtcgtggcc gagacgtatg agcaggcgcg
     1501 ctacgccgcg cgcctcgtta aagtctccta caacgcggag aagcaccaga ccgacacgga
     1561 atcggtgcgc gaccgcgcgc gcgctccctc gcaggggccg ccaccgaagc cccgcggcaa
     1621 ccccgacgcg gcgctgcgct cggccccggt gaaggtcgag gccgagtacc gcatcgccgt
     1681 cgagcaccac aacccgatgg agccgcacgc ggccgtcgcc gcatgggagg gagacaagct
     1741 caccgtcttc gacaagacgc aggaggtcta cagcgtgcgg acgcacctcg cgtcgagctt
     1801 c
//
LOCUS       DBNGOH010000755         3075 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3412704, whole genome shotgun sequence.
ACCESSION   DBNGOH010000755 DBNGOH010000000
VERSION     DBNGOH010000755.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3075)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3075)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3075
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3412704"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..947)
                     /gene="kduI"
                     /locus_tag="AC159P_14540"
     CDS             complement(<1..947)
                     /gene="kduI"
                     /locus_tag="AC159P_14540"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013533712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HIR3696716.1"
                     /translation="MAETCLEFEPTHFTQMAGLVCLYNTRMHFYARATHDEGAGKVLG
                     VVVTDGGVYDELAVGPVLDASKLSDDTDWREGYMYRKTYYATHPASVQGAANDELRER
                     YLLSELFAEGELRLNYLHYERFVIGGAAPVKEPVSLPLQQEPASAKDQPFLERRELGA
                     VNVGAGRGAVTVDGQRFVLQPKDGLYIPMGSAEVSFESEDEGSPAKYYLASTPAHARF
                     EVVHVSVEKAVPLRLGSAETSNERTIYQYVVPAVCKSSQLLLGLTMLKPGSVWNTCPP
                     HLHDRRSEVYFYFDLAKGQRVMHFMGELEEMRLITMGGD"
     gene            1100..1537
                     /locus_tag="AC159P_14545"
     CDS             1100..1537
                     /locus_tag="AC159P_14545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiD C-terminal domain-containing protein"
                     /protein_id="HIR3696717.1"
                     /translation="MNTARQLEELFYAKIPVTRALGVRVEEYDGRRLVLTAPLRENVN
                     HLGTAFGGSINALAVLSGYGLLWLELRDTQCHIVIRESAIHYDRPVRGDLRAVCVRPG
                     EEAVEAFKHQYRQRGKARIVLEATVEDGGATAVRFRGTFVAMR"
     gene            2317..2919
                     /locus_tag="AC159P_14550"
     CDS             2317..2919
                     /locus_tag="AC159P_14550"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3696718.1"
                     /translation="MASKNPRSYEDYMASLTSRYRKAARQIEKEVAGAGCTVEELPDA
                     GRHAAELHELYMQTHRNARLRLVTLPEGFIPTLAARLGDEMRCTVVRRGEELLGFVTT
                     VRDGETAVGYYIGFDRGANAELPVYFRLLQSVVDDAIRLGCRRLSLGRTALEPKARLG
                     ARPAPMRVWIRHRIPALNVVVRALLHTVPHDEAPERSPFK"
BASE COUNT          580 a          950 c         1006 g          539 t
ORIGIN      
        1 tcgtcgccgc ccatcgtgat gaggcgcatc tcctccagct cgcccatgaa gtgcatcacc
       61 cgctgcccct tcgcgaggtc gaagtagaag tagacctccg agcgccggtc gtgcaggtgc
      121 ggggggcagg tgttccagac gctgcccggc ttgagcatgg tgaggccgag gaggagttgt
      181 gaggacttgc agacggcggg cacgacgtac tgatagatcg tccgctcgtt cgaggtctcc
      241 gcggagccga gccggagcgg cacggccttc tccacagaga cgtggacgac ctcgaagcgc
      301 gcgtgcgcgg gcgtcgaggc aaggtagtac ttcgcggggc tgccctcgtc ttcgctctcg
      361 aacgagacct cggcgctccc catggggatg tagagaccgt ccttcggctg caacacgaag
      421 cgttggccgt ccacggtcac ggcgccgcgc cccgcgccga cgttgacggc gcccagctcg
      481 cgccgttcga ggaagggctg gtctttggcc gaggctggct cctgctgcaa cgggagcgaa
      541 acaggctctt tgacgggcgc cgcgccgccg atgacgaagc gctcgtagtg caggtagttc
      601 agcctcaact cgccttcggc gaacaactcg ctgaggagat aacgctcgcg cagttcgtcg
      661 ttcgcagcgc cttgcaccga agccgggtgc gtggcgtagt aggtcttgcg gtacatgtat
      721 ccttccctcc aatccgtgtc gtcggaaagt ttcgaggcgt cgagcaccgg cccgacggcc
      781 agttcgtcgt aaaccccgcc atctgtgacc acgacgccga gcactttgcc cgcgccttcg
      841 tcgtgcgtgg cgcgagcata gaaatgcatt cgcgtgttgt aaaggcagac gaggccggcc
      901 atctgcgtga agtgcgtcgg ctcgaactca agacaggtct cggccactca gcggaagccc
      961 gcaattctcg ccggcgacac ggagaagccc cgcgggctat gtgccgggcg gcgaggcgta
     1021 gaacacggca agttactctg accgagccgc aaaggcggac gggatacaca tgtcaggtca
     1081 actcccgtaa catgtccgca tgaacaccgc acggcagttg gaagagctgt tctatgccaa
     1141 gatacccgtc acgcgcgcgc tcggcgtgag ggtcgaggag tacgacgggc ggcgactggt
     1201 gctgacggct ccgctgcgcg agaacgtcaa ccacctgggc acggccttcg gcgggagcat
     1261 caacgcgctg gcggtgctga gcggctacgg gctgctgtgg ctggagctgc gcgacacgca
     1321 gtgccacatc gtcatccgcg aaagcgccat ccactacgac cggcccgtgc ggggagacct
     1381 gcgcgccgtc tgcgtccgcc ccggcgagga ggcggtggag gcgttcaaac atcaataccg
     1441 acagaggggc aaggcgcgca tcgtcctcga agctacggtc gaggacgggg gcgcgacggc
     1501 ggtgcgtttc cgcgggacgt tcgtcgccat gcgctagcgc gcatgaccgg gctggagatg
     1561 attaacgaac aagcgggcac caaattctac tacgacctcg ctttgcccga cagaacggca
     1621 gccgacccaa gatgatgagg ccgcacctgc tgccgcactc gatatcgagt accgacttcg
     1681 attcgttggg caaagcgagg acgcgctcta tttcgacggg tgtgtctcgc ggatgtacct
     1741 tcccgcgcac ggcgctcata tccccgagcg gcgacaatgt gataaagagt tggtatggtt
     1801 gttaggcgcg gcgaggcggg agtaaggagg tcatccccga gttatattct gcctgccgct
     1861 cgttatttag gctcaagcag ttcccttcca aaacttgtga gtcggtgaat gatcttgcgt
     1921 acattaatag catttacgat cggagtcctc ctacaggtgc aaggtggccc cggttacgtc
     1981 tttgcccaaa caggtaacat gacccgccag gaaatgcttc gaggctcggt aacacccgag
     2041 cgcgagtggt gggacgtgct gcattatcac ctgaaggtcg agtttttccc tgagaccaga
     2101 aggctgaaag ggtcgaacgc gataactttc aagtctctga aagcgggaga caaaatgcag
     2161 atcgatttgc agccgccgct tgcgatcacg aaagtcactc acggcgactc tcagctcgaa
     2221 ttcgagcgcg aagggactgt ctattgggta actttcgaga aggacattcc gaaaggcgtg
     2281 gaagataagg tcgaagtatt ttacgaaggc gtcccggtgg cgagtaagaa tccgcggagt
     2341 tatgaagact acatggcgag ccttacttcc aggtatcgca aggcggcgcg gcagatcgag
     2401 aaggaggtgg cgggggccgg gtgcacggtc gaagagctgc cggacgcggg gcggcacgcc
     2461 gcggagttgc acgagctgta catgcagacg caccgcaacg cgcggctgcg tctggtgacg
     2521 ctccccgaag ggttcatccc gacgctcgcc gcgcggctcg gcgacgagat gcgctgcacg
     2581 gtcgtccgtc gcggcgagga actgctgggc ttcgtgacga cggtgcgcga cggcgagacg
     2641 gctgtcggct actacatcgg cttcgaccgc ggggccaacg ccgagctgcc cgtctacttc
     2701 cgcctgctcc aatcggtcgt ggacgatgcc atccggctcg gctgccggag gctctctctg
     2761 ggccgcaccg cgctcgaacc gaaggcgcgg ctcggcgcgc gccccgcgcc catgcgcgtc
     2821 tggattcgcc accgcatccc cgccctcaac gtcgtcgtgc gcgcgctcct ccacaccgtc
     2881 ccgcacgacg aagcgcccga gcgcagccct ttcaaataaa gaggcaaagc tgacgcggct
     2941 gcttgtgtat aatccgacgc cgtggacggt gcggctgctt cgtgataacc cgccttcgtt
     3001 cggcatgaca caccggcttc ggacttggaa acccgcatga tgcacacacc acgcacggcg
     3061 ctgcccgcgg tcgcc
//
LOCUS       DBNGOH010000756         7133 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_845947, whole genome shotgun sequence.
ACCESSION   DBNGOH010000756 DBNGOH010000000
VERSION     DBNGOH010000756.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7133)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7133)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7133
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_845947"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="AC159P_14555"
     CDS             <1..192
                     /locus_tag="AC159P_14555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3696719.1"
                     /translation="IEEAAQLADRVVVLSERPAVINRQLRVTLPRPRELTHPEVVETV
                     RRTLAALGLEQEEMMNAER"
     gene            269..1291
                     /locus_tag="AC159P_14560"
     CDS             269..1291
                     /locus_tag="AC159P_14560"
                     /inference="COORDINATES: protein motif:HMM:NF024772.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR3696720.1"
                     /translation="MLRSSVARWAVGLVVVILVLGLMRFKPWQRGGAPAAREQLQVGF
                     LPVTCHLTCPVTDYATRTSTATRFESQRFTDFPTIVESIKGGRLEATFMIAPLAMKLR
                     EQGVPVKIAYLGHRDGSTVMVRSDLTEVKDLRGLRGRTFAIPSKYSNQYLVIRKLMDD
                     QGIGPDEVRFVELPPPDMPGALASKAIDAYFVGEPHPAKAELDGTGRILYHAKDIWPQ
                     FISCVLVVHERLIRERPEVVRDLVRGIAESGEWAERNRLDAAKVASPYFRQDERVVRH
                     VLTQPPDRVSYRMLTPTDEDLQKIHDMALKAGILERPISMKDLIDRRFIPPDIKPAPI
                     TVDGGQ"
     gene            1388..2494
                     /locus_tag="AC159P_14565"
     CDS             1388..2494
                     /locus_tag="AC159P_14565"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013334561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HIR3696721.1"
                     /translation="MNKPEIRALPRRRFIERLTTGAVLAVAGALRPNLLPSAQAAAPA
                     KTYTNPVYTGSMPDPFVLRHRGVYYAFGTTGGERKTDGRIYTLLRSTNLVDWRELGGA
                     LVAPSADTAYQYWAPEVAYHGGTFYLYYSMGGKEEEKFELRVATSRRPEGPYKDAGTK
                     LLDCEDNRFTIDAHPFRDTDGRWYMFYARNFIDTEGGAHPGTALVVDRLVGMTRLAGE
                     CRTVLRARHPWTLYEANRRMEVYGKTFDWHTIEGAFVRKHGGRYYCFYSGSNWQTPNY
                     GVDYAVADRVTGPYAGQGAEARVLKGVPGKVRGPGHHSIVTGPDGKTEYFVYHAWDDA
                     MKVRQMCIDRLVWTPAGPRLDGPTYTPQPRPALR"
     gene            3049..3882
                     /locus_tag="AC159P_14570"
     CDS             3049..3882
                     /locus_tag="AC159P_14570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HIR3696722.1"
                     /translation="MNRRSFFSQSFGALCLAGRGAAKLNASERRLTNIGLQLYTVRRE
                     LATDFEGTLAKVAALGFRAVEFAGYHGRAPGEVKAALVRQRLTAPSAHVTTSALRGQL
                     RETIEAAKIIGHEYLVCGHVPAEERRSLDDYERLADLLNRSGEQTKKAGIQFCYHNHD
                     FEFERVGGETPYDLLLAQTDARLVKLELDLYWITKAGHSPLDYFAKHRGRFPLVHVKD
                     MDNTPRRFFTEAGRGVIDFKRIFAAASGVKHYFVEQDETPASPFESIRVGMDYLKRLR
                     F"
     gene            3989..4252
                     /locus_tag="AC159P_14575"
     CDS             3989..4252
                     /locus_tag="AC159P_14575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696723.1"
                     /translation="MTKQLPTGQTPLGEREYEVWADDSYPPYILVVIREEAEPPTFKV
                     LGPSERNHVVFSSTDYDEVWWWLQEDEFHRVEGRAKILEWWEQ"
     gene            4509..6359
                     /locus_tag="AC159P_14580"
     CDS             4509..6359
                     /locus_tag="AC159P_14580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009579342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HIR3696724.1"
                     /translation="MRLGTLDVAVLIVYFVAMIGVGVYVTRRAAKNLDSYFLGGKSMP
                     WWLLGVSNASAMWDITGTMWFVYILYAYGMKAVFLPWLWPIFNQVFEAVYLSKWVRRS
                     NARTGAEWITTRFGAGRGGELARAVIVLFALIGVVGFISYDFQGMGKFCKVFLPWDLS
                     PATYAVIIMSITAVYVVLGGMLSVVLTDFVQFILMALSSVVIAVVAMTNVGPDALARA
                     TPAGWRGLAFGYDITLDWSGLLPAMNDRVAAEGIASAFGLFFVVMVFKGILASMAGMA
                     PNYDMQRVLAARSPREASLMSAIVSVCLVPRWFLIGGITLLGLVYVTPEFRTMGDRID
                     FELVLPYVINNFLPAGLVGILLAGLLSAFMSTFSATVNAGAAYLVNDVYKRYLKPEAG
                     DRHLVRASYVGSVLILVVGMLVGSMVSSIHDITQWIVSGLYGGYTAANVLKWYWWRLN
                     GHGYFWGMVTGIVCAMLVPVVAPALNPLYAFPFILLLSAAASVAGSLLTRPVDEETLK
                     SFYRDVRPWGFWGPVHALAAQDDPSIRPNRDAARDLTNCGVGIVWQLTLVVIPLYVVF
                     RDWRGVLISLAVLAATTVFLKRFWYDRLEPGEGQAEGAGRLELEAERVPS"
     gene            6356..>7133
                     /locus_tag="AC159P_14585"
     CDS             6356..>7133
                     /locus_tag="AC159P_14585"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF053331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase 5 family protein"
                     /protein_id="HIR3696725.1"
                     /translation="MKRASRLLTLVSALAVFSSAAFAQDDMRHFVKRSGDRLSEGGKE
                     YRFISFNIPNLHLVEDNMAFEATNEWRFPDEFEITDALLSVRQMGGRVVRTYVISVCR
                     KEGANPIPCHVKRPGEFNEEAFRALDKVLEVANRVGVRVVIPLVDNWKWWGGVEQYAE
                     HRGLPAEAFWTDRRLVEDFKQTVSFLLKRRNTYTGTLYKDDRAVLAWETGNELYSPYP
                     WTKEIAAHIKSLDPNHLVWDGFYIGNREIQPEALADPNIDI"
BASE COUNT         1239 a         2373 c         2291 g         1230 t
ORIGIN      
        1 atcgaggagg cggcccaact cgccgaccgc gtcgtggtgc tctccgagcg cccggcggtc
       61 atcaaccgcc agctccgcgt cacgctcccg cgcccgcgcg agctgacgca ccccgaggtg
      121 gtcgagaccg tgcgccgcac cctcgccgcg ctcggtctgg aacaggaaga aatgatgaat
      181 gcagaacgat gaatgatgaa tcaaaagcag tttgtctttc attcatcatt ccgcactctg
      241 cattcatcga ttgacttgag gagggttcat gcttcgttct tcggtggcgc gctgggcggt
      301 cggcctcgtc gtggtgatct tggtgctggg gctgatgcgg ttcaagccgt ggcagcgggg
      361 cggcgcgccc gccgcgcgcg agcagttgca ggtcgggttc ctgccggtga cgtgccacct
      421 gacgtgccct gtgacggact acgcgacgcg gacgagtacg gcgacgcggt tcgagtcgca
      481 gcgcttcacg gactttccga ccatcgtcga gtcgatcaag ggcgggcggc tcgaagcgac
      541 cttcatgatc gcgccgctgg cgatgaagct cagggagcag ggcgtccccg tcaagatcgc
      601 ctacctcggc caccgcgacg gctcgaccgt catggtgcgc tccgacctca ccgaagtgaa
      661 agacctgcgg ggcttgcgcg ggcgaacctt cgccatcccg tcgaagtaca gcaaccagta
      721 tctcgtcatc cgcaagctga tggatgatca gggcatcggc cccgacgagg tgcgcttcgt
      781 cgagctgccg ccgcccgaca tgcccggcgc gctcgcctcg aaggcgattg acgcctactt
      841 cgtcggcgag ccgcacccgg ccaaggccga actcgacggc acgggccgca tcctttacca
      901 cgccaaagac atctggccgc agttcatctc ctgcgtgctg gtcgtccacg aacggctcat
      961 ccgcgagcgg cccgaggtgg tgcgcgacct ggtgcgcggc atcgccgaga gcggcgagtg
     1021 ggccgagcgc aaccgcctcg acgccgcgaa ggtggcctcg ccctacttcc ggcaggacga
     1081 gcgcgtcgtg cgccacgtgc tcacgcagcc gcccgaccgc gtcagctacc ggatgctcac
     1141 gcccacggac gaagacctcc agaagattca cgacatggcg ctcaaggccg gcatcctcga
     1201 acgccccatc tcgatgaaag acctgatcga ccgccggttc atcccgcccg acatcaagcc
     1261 cgcgccgatc accgtggacg gcggtcaatg agagagcctt gacgggaggc gccgtggtcg
     1321 aacgctcgaa cgaaggcggc gcgcccgtat cgacgccgcg cgaagaacca ttcatggagc
     1381 cgtcgagatg aacaagccgg agattcgcgc gctgccgcgc cgtagattta tcgaacgact
     1441 gaccacgggc gcggtgctcg ccgtcgccgg ggcgctacgt ccgaacctcc tgccgtccgc
     1501 acaggccgcc gcgcccgcga agacctacac gaacccggtc tacacaggct cgatgccaga
     1561 cccgttcgtg ctgcgtcacc gcggcgtcta ctacgccttc ggcacgacgg gcggcgagcg
     1621 caaaacagac ggtcgcatct acaccctgct ccgctcgacg aacctcgtgg actggcgcga
     1681 gctgggcggc gcgctcgtgg ccccctccgc cgacacggct taccaatact gggcacccga
     1741 ggtcgcttac cacggcggca cgttctacct ctactactcg atgggcggga aggaggagga
     1801 gaagttcgag ctgcgcgtcg ccacgagccg ccggcccgaa ggcccgtaca aagacgcggg
     1861 caccaaactc ctcgactgcg aggacaaccg cttcaccatc gacgcccacc cgttcaggga
     1921 cacggacgga cgttggtaca tgttctacgc gcgcaacttc atcgacaccg agggcggcgc
     1981 gcatccgggc acggcgctcg tcgtggacag gctcgtcggg atgacgcggc tcgcgggcga
     2041 gtgccgcacg gtcttgcgcg cgcgccaccc ctggacgctc tacgaggcca accgccggat
     2101 ggaggtctac ggcaagacgt tcgactggca caccatcgag ggcgcgttcg tacgcaagca
     2161 cggcggtcgc tactactgtt tctacagcgg ctcgaactgg cagaccccga actacggcgt
     2221 ggactacgcc gtggccgacc gcgtgaccgg gccttacgcg ggtcagggcg cggaggcgcg
     2281 cgtgctgaag ggcgtccccg gcaaggtgcg cggccccggc caccacagca tcgtcacagg
     2341 gccggacggc aagaccgaat acttcgtcta ccacgcctgg gacgacgcga tgaaggtgcg
     2401 gcagatgtgc atcgaccggc tggtgtggac gcccgcgggg ccgcgcctcg acggcccgac
     2461 ctacacgccg cagccgcgcc ctgcgctcag gtagtcccgt tcatcccatg ccacgccggg
     2521 gcaggtcatc ggcgaccagg acggcacgtg ggacaagacc gcatggaaga ccgacggcat
     2581 ctacggcaac tccctgaccg gcttcacggc cttgccataa acgcgcgggc ggcgtcttga
     2641 cttttcaagt caagacgccg cagtaaccag tcgcgcaatt cgcctgagcg ccaaatagaa
     2701 ccgccctaag gtctgtaacg actgacgggc ggttctgctg ttatcactca gccccacgaa
     2761 cggagataga ggacggggtg acggcccgcc ccggccccgt gatcgtctca acgcgtcccg
     2821 aagcggttga tccgccactt gacgcgaccg cggacagcag acccgtcaac agcgaggcga
     2881 agacccgcag ccgggccgtc tcccgcacgc tttcaatcat ctcgtgtttc aactctcttc
     2941 cctccgtccg cgccccgcgg ctgtacaccc ggcgcatcct ggctaaaata cacaccccgc
     3001 gggaaacttt catcattatc gtcgagccgg gaggcacgtg ctgattccat gaatcgtcgc
     3061 tcatttttca gtcagtcatt cggcgcgctg tgcctcgcgg ggaggggcgc ggcgaagctc
     3121 aacgcttccg agcgcaggct cacgaacatc ggccttcagc tttacaccgt gcggcgggag
     3181 ttggcgaccg acttcgaagg cacgctggcg aaggtcgcgg ccctcgggtt tcgcgcggtc
     3241 gagttcgcgg gctatcacgg acgcgcgccg ggcgaggtca aagccgcgct cgttcgccaa
     3301 cggctcacgg ccccttccgc gcacgtcacg acctcggcgc tgcgcggcca actgcgcgag
     3361 accatcgagg ccgccaagat aatcggccac gagtatctcg tgtgcggcca tgtgcccgcg
     3421 gaggagcgcc gctcgctcga cgactacgag aggctggccg accttctcaa ccgctccggc
     3481 gagcagacga agaaggcggg catccagttc tgctaccaca accacgactt cgagttcgag
     3541 cgcgtgggcg gcgaaacgcc ttacgacctc ttgctcgcgc aaacggacgc gcggctcgtg
     3601 aagctggagc ttgatctgta ctggatcacg aaggcggggc acagtccgct cgactacttc
     3661 gcgaagcaca gggggcgctt cccgctcgtg cacgtcaaag acatggacaa cacgccgcgg
     3721 cgcttcttca cggaggcggg gcgcggcgtg atagatttca agcgcatctt cgccgccgcc
     3781 agcggcgtca agcactactt cgtcgagcag gacgagacgc ccgcctcgcc cttcgagagc
     3841 atacgggtcg gcatggacta cctgaagcgg ctcaggttct gaacgccgcg ccgggtcggc
     3901 ccgcgaaggc tgctcgcaaa aaggcggacg gcgtctctcg gaagctttgg gagattgaag
     3961 atattgtagc ttcgccggat gagtgattat gacaaagcaa cttccgaccg ggcagacgcc
     4021 gttaggcgaa agggagtatg aagtgtgggc tgatgactcc tacccgccat atattctcgt
     4081 cgtcatcagg gaggaagctg agccgccgac gttcaaggtt cttggcccat cggagcgaaa
     4141 tcatgtggtg ttctcttcga ccgattatga tgaggtatgg tggtggttac aagaggacga
     4201 gttccaccgg gtcgagggtc gcgctaaaat cttggaatgg tgggagcagt aaaagccctt
     4261 tggttcggaa tccgaactga accactaccc gcaaaaaact gtcattgcaa atccgcggca
     4321 tctcggatat agaatcgcca cgcgttgtac ggccctcacc gtttccgttt cgagaagcac
     4381 aaacgaaccc tcggctcgta agaccgagcg accagtgtgt ctcgcggcac cccgtcccga
     4441 gttgcggcga gcattgaccg gacacccgac aaaagccggc gcgcggcatt aagggaggag
     4501 gagatgacat gcggttgggc acgctcgatg tcgccgtcct gatcgtctat ttcgtcgcca
     4561 tgatcggcgt cggcgtctac gtcacgcgcc gggcggcgaa gaatctggac tcctacttcc
     4621 tcggcggcaa gagcatgccg tggtggctgc tcggcgtctc gaacgcctcg gcgatgtggg
     4681 acatcacggg cacgatgtgg ttcgtctaca tcctctacgc ctacgggatg aaggccgtct
     4741 tcctgccgtg gctgtggcct atcttcaatc aggtcttcga ggcggtctac ctgtcgaagt
     4801 gggtgcggcg ctcgaacgcg cgcaccggcg ccgagtggat cacgacgcgc ttcggcgcgg
     4861 gccgcggcgg cgagttggcg cgcgccgtca tcgtgctctt cgcgctgatc ggcgtggtcg
     4921 gcttcatcag ctacgacttt caggggatgg gcaagttctg caaggtcttc ctgccgtggg
     4981 atctgtcgcc cgcgacttac gccgtcatca tcatgtcgat cacggcggtc tacgtcgtcc
     5041 tcggcgggat gctgagcgtg gtgctgacgg acttcgtgca gttcatcctg atggccctct
     5101 ccagcgtcgt catcgccgtc gtcgcgatga cgaacgtcgg ccccgacgcg ctcgcgcggg
     5161 cgacgcccgc gggctggcgc ggactcgcgt tcggctacga catcacgctc gactggtcgg
     5221 gactgttgcc cgcgatgaac gaccgcgtcg cggcggaggg catcgcctct gccttcggcc
     5281 tcttcttcgt ggtgatggtc ttcaaaggca tcctcgccag catggcgggc atggccccca
     5341 actacgacat gcagcgcgtg ctggcggcgc gcagcccgcg cgaggccagt ctgatgagcg
     5401 ccatcgtctc ggtctgcctc gtcccgcgct ggttcctcat cggcggcatc acgctgctcg
     5461 ggctggtcta cgtcacgccc gagttcagga cgatgggcga ccggatcgac ttcgagctgg
     5521 tgctccctta cgtcatcaac aacttcctgc ccgcgggtct cgtcgggata ctgctggcgg
     5581 ggctgctctc ggccttcatg tccaccttca gcgcgacggt caacgcgggg gccgcctacc
     5641 tcgtcaacga tgtatacaag cgttacctca agcccgaggc cggagaccgc cacctcgttc
     5701 gcgcgagcta cgtcgggtcg gtgctgatcc tcgtcgtcgg catgctcgtc ggctcgatgg
     5761 tctcttcgat ccacgacatc acgcagtgga tcgtgtcggg gctgtacggc ggctacacgg
     5821 cggcgaacgt gctgaagtgg tactggtggc ggctgaacgg ccacggctac ttctggggga
     5881 tggtgacggg catcgtctgc gccatgctcg tgccggtcgt cgcccctgcg ctgaacccgc
     5941 tctacgcctt tcccttcatc ctcctgctct cggcggcggc ctcggtcgcg ggcagcctgc
     6001 tgacacggcc cgtggacgaa gagacgctga agagcttcta ccgcgacgtg cggccctggg
     6061 gcttctgggg gccggtgcac gcgctggccg cgcaggacga cccctcgatc cgcccgaacc
     6121 gcgacgccgc gcgcgacctc accaactgcg gcgtcggcat cgtctggcag ttgacgctcg
     6181 tcgtcattcc gctctacgtg gtcttccgcg actggcgggg cgtgctcatc tcgctcgcgg
     6241 tgctggcggc gaccacagtc ttcctcaagc ggttctggta cgaccgcctc gaaccgggcg
     6301 aaggacaggc ggagggcgcg ggccgtcttg agcttgaagc ggagcgggtg ccgtcatgaa
     6361 gcgtgcgagc cgattgctga cgttggtctc ggcgctggcg gttttctctt ccgccgcttt
     6421 cgcgcaggac gacatgcggc acttcgtcaa acgctccggc gacaggctgt cggagggcgg
     6481 caaggagtac cgcttcatct ccttcaacat cccgaacctg cacctcgtcg aagacaacat
     6541 ggccttcgag gcgacgaacg agtggcgctt tccggacgag ttcgagatca cggacgcgct
     6601 cttgagcgtc cgccaaatgg gcgggcgggt cgtgcggact tacgtcatct cggtctgccg
     6661 gaaggagggc gcgaacccga tcccctgcca tgtgaagagg ccgggcgagt tcaacgagga
     6721 ggctttccgc gcgctcgaca aggtgcttga ggtggcgaac cgcgtcggcg tccgcgtggt
     6781 catcccgctc gtcgataact ggaagtggtg gggcggcgtc gagcagtacg cggagcaccg
     6841 cgggttgccc gccgaagcct tctggaccga ccgccggctc gtcgaagatt tcaagcagac
     6901 cgtcagcttc ctcctcaaac ggcgcaacac ctacacgggc acgctctaca aagacgaccg
     6961 ggccgtcctc gcgtgggaga cgggcaacga gctgtacagc ccttacccgt ggacgaagga
     7021 gatcgccgcg cacatcaagt ctctcgaccc gaaccacctc gtctgggacg gcttctacat
     7081 cggcaaccgt gagattcagc ccgaagccct cgccgacccg aacatcgaca tcg
//
LOCUS       DBNGOH010000757         2160 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4328545, whole genome shotgun sequence.
ACCESSION   DBNGOH010000757 DBNGOH010000000
VERSION     DBNGOH010000757.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2160)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2160)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2160
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4328545"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..412)
                     /locus_tag="AC159P_14590"
     CDS             complement(<1..412)
                     /locus_tag="AC159P_14590"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007799919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HIR3696726.1"
                     /translation="MIPFVDLKAQYRSIREEVGEAIERVLEGTQFILGEEVEAFESEF
                     AAYCGARHAVGVNSGTSALHLALLAAGVREGDEVVTVSHTFVATAAAVRYCGATPVFV
                     DIDPRTCLIDPDRIEAAITPRTKAIMPVHLYGQCA"
     gene            complement(490..942)
                     /locus_tag="AC159P_14595"
     CDS             complement(490..942)
                     /locus_tag="AC159P_14595"
                     /inference="COORDINATES: protein motif:HMM:NF026766.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm26 family immunity protein"
                     /protein_id="HIR3696727.1"
                     /translation="MARKFQKYSVGGVLRIPLGGEWHTYGQMTGDAEIAFFDAKTRTD
                     LPVRDVITRPVLFRVAVDSYAVTKLVWLKVGKAPLPDALKVPQPQYIQDALHPERFEL
                     YLGGKIRPATREECVGLECCAVWSPEQVVDRLNDHYNGVPCKWLRRAI"
     gene            complement(984..2015)
                     /locus_tag="AC159P_14600"
     CDS             complement(984..2015)
                     /locus_tag="AC159P_14600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015111071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR3696728.1"
                     /translation="MLRVGVIGYGYWGPNLVRNFMEAQGATVAAVCDLSVGRLAPLGT
                     RYPTVRLTTDYRELLADSRVDAILIATPVASHFELGMAALRAGKHVLVEKPLAANSEQ
                     ALRLVEEAERRGLVLMVDHTFIYTGAVRKIRELIAGGALGEIYYYDSVRINLGLFQHD
                     VNVVWDLAVHDLSIMDYVLPARPVAVSATGISHLPGQPENVAYITLFFAGSLIAHVHV
                     NWLSPVKVRRTLIGGSEKMIVYDDLEPSEKLKVYDKGVTVGQHPEDVYELLVSYRSGD
                     MWAPRFDTTEALRTEAHHFVECVEKATRPETDGLEGLRIVRIMEAAEQSLRDRGKLVE
                     LPADVGALV"
BASE COUNT          374 a          701 c          699 g          386 t
ORIGIN      
        1 cggcgcactg gccgtagagg tggacgggca tgatggcctt cgtgcgcggc gtgatcgccg
       61 cctcgattct gtcggggtcg atcagacagg tgcgcgggtc gatgtccacg aagacgggcg
      121 tggcgccgca gtagcgcacc gccgcggcgg tggcgacgaa cgtgtggctg acggtgacga
      181 cctcgtcgcc ctctcggacg cccgccgcga gcagcgccag gtgcagcgcg ctcgttcccg
      241 agttgacgcc gaccgcgtgg cgcgcgccgc agtaggccgc gaactccgac tcgaacgcct
      301 cgacctcctc gccgaggatg aactgcgtgc cttccagcac gcgctcgatg gcctcgccga
      361 cctcttccct gatgctccgg tactgtgctt ttaaatcgac gaaaggaatc ataattcaag
      421 agcggttagc tgccggctgt gtctcgcgcg gcaagtcaac gccggttcgc ggtcgggtgc
      481 gtgccgttgt cagatggcgc gtcggagcca cttgcaaggt acgccattgt aatggtcgtt
      541 gaggcggtca accacttgct ccggcgacca cactgcgcaa cactccagcc cgacgcactc
      601 ctctctggtc gccgggcgaa tcttgcctcc gaggtatagc tcgaagcgct cgggatggag
      661 cgcgtcttga atatactgcg gctgcgggac tttcaaggcg tccggcaaag gcgccttccc
      721 caccttcagc cacacgagct tggtgacggc ataactatcc acagccaccc tgaacaagac
      781 ggggcgcgtg ataacgtcac gcacgggtaa atccgttcgg gttttagcgt cgaagaaggc
      841 gatctcggcg tcgccggtca tctgcccgta ggtatgccat tcgcccccga gaggtatcct
      901 gaggacgccg cctacggaat acttctggaa ctttcgagcc accgtcgaaa ccaagaagcc
      961 ggttaacggc taacagccct tcctcacacc agtgcgccca cgtccgcggg cagctcgacg
     1021 agcttgccgc ggtcgcgcag cgattgttcg gcggcctcca tgatgcggac gatgcgcaga
     1081 ccttcgaggc cgtcggtctc ggggcgcgtg gccttctcga cgcactcgac gaagtggtgc
     1141 gcctcggtgc ggagcgcctc ggtcgtgtcg aagcgcggcg cccacatgtc gccggagcgg
     1201 tagctgacca aaagctcgta aacgtcctcg gggtgctggc cgacggtgac gcccttgtcg
     1261 tagaccttga gcttctcgct cggctcaagg tcgtcgtaga cgatcatctt ctcgctgccg
     1321 ccgatgagcg tgcggcgcac cttgacgggc gagagccagt tgacgtggac gtgcgcgatc
     1381 aggctgcccg cgaagaagag cgtgatgtag gcgacgttct cgggctggcc cggcaggtgg
     1441 ctgatgccgg tcgcggagac ggcgacgggc cgggccggca gcacgtagtc catgatcgag
     1501 aggtcgtgca cggccaaatc ccagaccacg ttcacgtcgt gctggaacag cccgaggttg
     1561 atgcgcaccg agtcgtaata gtaaatctcg cccagagcgc cgcccgcgat cagctcgcgg
     1621 attttacgca ccgcgcccgt gtagatgaac gtgtgatcga ccatcagcac gaggccgcgc
     1681 cgctcggcct cctcgaccag ccggagcgcc tgctcggagt tcgccgccag aggcttctcg
     1741 acgagcacgt gcttgcccgc gcgcagcgcc gccatcccca gctcgaagtg cgaggcgacg
     1801 ggcgtggcga tcagtatcgc gtccacgcgc gagtccgcca gcagctccct gtagtccgtc
     1861 gtcaggcgca cggtcgggta gcgcgtcccg agaggcgcca gccgccccac cgacaggtcg
     1921 cacaccgcgg ccacggtcgc cccctgcgcc tccatgaagt tgcgcacgag gttcggcccc
     1981 cagtagccgt atccgatgac gccgacgcga agcataaaaa tcagtaggca gtaggcagta
     2041 ggcagtaggc agcgggcagc gggtagttgg ccattgactc ttcagttggt tgaacgttaa
     2101 tggtcatgct cacagtgctc cctttaaggc gaaagttttt tgtctttact gcctactgcc
//
LOCUS       DBNGOH010000758         3293 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3413232, whole genome shotgun sequence.
ACCESSION   DBNGOH010000758 DBNGOH010000000
VERSION     DBNGOH010000758.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3293)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3293)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3293
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3413232"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..701
                     /locus_tag="AC159P_14605"
     CDS             <1..701
                     /locus_tag="AC159P_14605"
                     /inference="COORDINATES: protein motif:HMM:NF019339.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3696729.1"
                     /translation="IINEILRAVAREYAWDEFLPPAVETITLAPEKLESYAGRYLINP
                     DRVFKVTREGARLFAETTQSPRFELLPITETTFIRTDANVRYTFAPAAEGKPATFSLR
                     QPDGSTPEAPRMAADAVIPYELLTGGKSAEAVEAYRKLKKEQPNNQAVSEQRLNQLGY
                     ELLRAGKKAESVAVFALNAELYPQSSNVYDSLGEAYMESGDKELAVKNYRRSLELDPK
                     NTNAVEMLKKLEQK"
     gene            865..1635
                     /locus_tag="AC159P_14610"
     CDS             865..1635
                     /locus_tag="AC159P_14610"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010046062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3696730.1"
                     /translation="MDVREYNRRGWDGEVERGNPWTVPVGPEVIEAARRGEWRVHLTE
                     QRYAPREWFPADMKGVRVLCLASGGGQQAPILAAAGARVTLLDNSPRQLDGDRMVAAR
                     DRLDIDIREGDMRDLSAFADESFDLVFHPVSNLFVPDVRPVWREAFRVLRRGGHLLSG
                     FLNPALYVFGSETEEHDKLEVRFTLPYSDLTSLTAEERARLLPNDEPVEFGHTLEDQI
                     GGQLDTGFLLDGFFEDRHVELRIAHYMPTYIATRAFKP"
     gene            1704..2291
                     /locus_tag="AC159P_14615"
     CDS             1704..2291
                     /locus_tag="AC159P_14615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018633285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2585 family protein"
                     /protein_id="HIR3696731.1"
                     /translation="MTNTNTPAGRRRRAWPWLAVAALLAATALVLRAQGRLWSCACGR
                     LLPWVSDAWGADTSQHFADPYTFTHVLHGVAFCGLLAWALPRLDWTRRFLLAVAAEAA
                     WEIVENTDFVIDRYREATAALGYTGDTVLNSLGDIAACALGFLLARKLGLWRSLIFFV
                     AVELLLVVWIRDSLLLNILMLLYPLDAVREWQAGK"
     gene            complement(2440..>3293)
                     /locus_tag="AC159P_14620"
     CDS             complement(2440..>3293)
                     /locus_tag="AC159P_14620"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR3696732.1"
                     /translation="FLVTYVNKVDLGQRPFTLWTDEGHEIKAETLIVASGASAKWLGI
                     PGEAPAPAGLGGLGVSACATCDGFFFKGKPIVVVGGGDTAMEEATFLTRYASQVTIVH
                     RRDHLRASKIMQDKAFNNPKIDFIWNTDVREIKGTPETGVTGVRLYNNRTGEESLYEC
                     EGVFVAIGHRPNTDAFKGWLEMDEVGYLKTAGRSMATNIPGVFACGDAQDSVYRQAVT
                     AAGTGCMAAIDAERFLDSLPVITPTGEEVTIEGERVTPDHQLVIKADGEVMPNHPDES
                     GGGAAVQ"
BASE COUNT          540 a         1185 c         1033 g          535 t
ORIGIN      
        1 agatcatcaa cgaaatcctg cgcgccgtcg cgcgcgagta cgcgtgggac gagttcctgc
       61 cgcccgcggt cgagaccatc acgctcgcgc ccgaaaagct cgaatcttac gcgggccgct
      121 acctcatcaa ccccgaccgc gtcttcaagg tcacgcgcga gggcgcgcgc ctcttcgccg
      181 agacgacgca gtcgccccgg ttcgaactcc tgcccatcac cgaaacgacc ttcatccgca
      241 ccgacgccaa cgtgcgctac accttcgcgc ccgccgccga aggcaagccc gcgaccttct
      301 cgctccggca gcccgacggc tcgactcccg aagccccgcg catggccgcc gacgccgtga
      361 tcccttacga gctgctgacg ggcggcaagt ccgccgaggc cgtcgaggct taccgcaagc
      421 tcaagaagga gcagccgaac aatcaggccg tctccgagca gcgtctgaac cagctcggct
      481 acgagctgct gcgcgccggg aagaaggccg aatcggtcgc cgtcttcgcc ctcaacgcgg
      541 agctgtaccc gcagtcgtcc aacgtctacg acagcctcgg cgaagcctac atggagagcg
      601 gcgacaaaga actcgccgtc aagaactacc gccgctcgct cgaactcgac ccgaagaaca
      661 cgaacgccgt cgagatgctg aagaagctcg aacagaagtg aggcgagtat gagaggcgaa
      721 aaggggaaga ggggaaaggg cgaaaaggga gaatgacgag cacggtgctt ctcatcatcg
      781 attcgatgac aggcacggtg cttcttgtcc ttccttttcc cctcttcccc ttttaacctt
      841 ttccccttcc gccgaggaga gtgcatggac gttcgtgagt acaaccggcg cgggtgggac
      901 ggcgaggtcg agcgcggcaa cccgtggacg gtgccggtcg gccccgaggt gatcgaggcc
      961 gcgcgcaggg gcgagtggcg cgtccacctg accgagcaaa ggtacgcgcc gcgcgagtgg
     1021 ttccccgcgg acatgaaggg cgtccgcgtg ctctgcctcg cctcgggcgg cgggcagcag
     1081 gcgccgatcc tcgccgccgc gggcgcgcgc gtgacgctgc tcgacaactc gccgcggcag
     1141 ctcgacggcg accgcatggt ggccgcgcgc gaccggctcg acatcgacat ccgcgaaggc
     1201 gacatgcgcg acctctccgc cttcgcggac gagagcttcg acctcgtctt ccaccccgtc
     1261 tcgaacctct tcgtgccgga cgtgcgcccc gtctggcgcg aggcgttccg cgtgctgcgg
     1321 cgcgggggcc acctgctctc cggcttcctc aacccggcgc tctacgtctt cggctccgaa
     1381 acggaagagc acgacaagct cgaagtccgc ttcacgctcc cctactccga cctcacgagc
     1441 ctcaccgccg aagagcgcgc gcggctcctg ccgaacgacg agccggtcga attcggccac
     1501 acgctcgaag accagatcgg cggtcagctc gacaccggct tcctcctcga cggcttcttc
     1561 gaagaccgcc acgtcgagct tcgcatcgcg cactacatgc cgacctacat cgccactcgc
     1621 gccttcaaac cctgaccgcc ccgaccgcag acgcatgacg ccgaaggcac gccacatccc
     1681 acacgccccg cgcggctgaa ccgatgacga acaccaacac tcccgccggg cgacggcgac
     1741 gcgcgtggcc gtggctcgcg gtcgccgccc tgctcgcggc gaccgcgctc gtgctccgcg
     1801 cccaggggcg cctctggtcg tgcgcctgcg ggcgcctgct cccgtgggtc tccgacgcct
     1861 ggggcgccga cacctcgcag cacttcgcag acccctacac cttcacgcac gtgctgcacg
     1921 gcgtcgcctt ctgcggcctg ctcgcgtggg cgctgccccg cctcgactgg acacggcgct
     1981 tcctgctcgc ggtcgccgcc gaggccgcgt gggagatcgt cgagaacacc gacttcgtca
     2041 tcgaccgcta ccgcgaggcg accgccgcgc tcggctacac cggcgacacc gtcctgaact
     2101 cgctcggcga catcgcggcc tgcgccctcg gcttcctcct cgcgcgcaag ctcggactct
     2161 ggcgctccct catcttcttc gtcgcggtcg agctgctgct cgtcgtctgg atcagggaca
     2221 gcctgctcct caacatcctc atgctcctct acccgctcga cgccgtcagg gagtggcagg
     2281 cggggaagta agcagtaggc agtaggcggt aggcagtagg cagtcaagac agaaggcagt
     2341 aaaagaaaga agaaggcagc cgtggacgcc aacgcgttac ggctgccttc ttcaattctt
     2401 cggctctgtt ttaactgcct actgcctgct gcctactgcc tactgtaccg cggcgccgcc
     2461 gccgctttcg tccgggtggt tgggcatgac ttcgccgtcg gctttgatga cgagctggtg
     2521 gtcgggcgtg acgcgttcgc cttcgatggt gacttcttcg ccggtggggg tgatgacagg
     2581 taaagagtcg aggaagcgct cggcgtcgat ggcggccatg cagccggtgc cggcggcggt
     2641 gacggcctgt cggtagacgg agtcctgcgc gtcgccgcag gcgaagacgc cggggatgtt
     2701 ggtcgccatc gagcgccccg cggtcttcag atacccgacc tcgtccatct ccagccagcc
     2761 cttgaaggcg tccgtgttcg gcctgtggcc gatggcgacg aagacgccct cgcactcgta
     2821 cagagactcc tcgcccgtcc ggttgttgta gagccgcacg cccgtcacgc ccgtctccgg
     2881 cgtccccttg atctccctca cgtcggtgtt ccagatgaag tcgatcttcg ggttgttgaa
     2941 cgccttgtcc tgcatgatct tcgaggcgcg caggtggtcg cggcggtgga cgatcgtgac
     3001 ctgcgaggcg tagcgcgtca ggaacgtcgc ctcctccatc gccgtgtcgc cgccgccgac
     3061 caccacgatc ggcttccctt tgaagaagaa cccgtcgcat gtcgcgcacg cagagacgcc
     3121 gaggcccccg aggcccgcgg gcgcgggcgc ctcgcccggg atgccgagcc acttggccga
     3181 ggcgcccgag gcgacgatca gggtctcggc cttgatctcg tgcccctcgt cggtccagag
     3241 cgtaaagggg cgctggccca ggtccacctt gttgacgtag gtgacgagga act
//
LOCUS       DBNGOH010000759         3940 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3413252, whole genome shotgun sequence.
ACCESSION   DBNGOH010000759 DBNGOH010000000
VERSION     DBNGOH010000759.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3940)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3940)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3940
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3413252"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(70..903)
                     /locus_tag="AC159P_14625"
     CDS             complement(70..903)
                     /locus_tag="AC159P_14625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HIR3696733.1"
                     /translation="MSSNPKPTSGALIKHKVKPGDTLTSIAKQHGVTLQALQSANPQI
                     ENINKIKIGQVVNVPASLVTEPAATNPTETGTGTQPTTPTVTTVTTVIPTVISTESEA
                     FKAMDKRGKTKKLNPVFRERLVLLAEALARRGMQTLITDGLRTFAEQDALFAQGRTKP
                     GNIVTKARGGQSNHNYGLAVDMYPVIGGKVFTDVPKGSSVEFRRTFNAIQDAAGEEAE
                     GLGLFWGARFSGIVDTPHIQLLPQTDMAPSECLKIFKRNGNSLDAVWAEAEKRVKPLT
                     A"
     gene            1314..1928
                     /locus_tag="AC159P_14630"
     CDS             1314..1928
                     /locus_tag="AC159P_14630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HIR3696734.1"
                     /translation="MKPLAAAFFALAVLAAAHVPPAEAATHRPLDPSADFQKRTARRK
                     SARRAPRRPAAPADAKQRPAPTSATTEKAETTAPQHQVVTGDSSTIGMTPQQAMAVAI
                     REALRKPREGETRVRGVLTLIECGPRNAAIFHVRAGAETLKLDGGDLRGISFTAFTPR
                     EAGSEIGCGPRKLDTPVVATYRPTAEGGDLVAVEFVPASFTLEK"
     gene            2092..2565
                     /locus_tag="AC159P_14635"
     CDS             2092..2565
                     /locus_tag="AC159P_14635"
                     /inference="COORDINATES: protein motif:HMM:NF015898.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HIR3696735.1"
                     /translation="MPKEPQVRLTRFELEVMEALWALGSASVREVQERLPARKRPAYT
                     TVQTIIYRLEEKGALRRVKKVGNAHVFEPVVSRKAAARRLVDELLDFFGGSPRTLMAQ
                     MVEGGRLTLEDLREAEGALERIEAERGTAGAAEEKGVGTPASGARRDAARERRKR"
     gene            2562..>3940
                     /locus_tag="AC159P_14640"
     CDS             2562..>3940
                     /locus_tag="AC159P_14640"
                     /inference="COORDINATES: protein motif:HMM:NF017390.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HIR3696736.1"
                     /translation="MSGPHEFLARASAWLWPALVDHLWQSALFAALVFAATLLVRRGP
                     ARARHALWLVALVKFAVPPALFAFLAARAGLDASWLFENQSAAATEYAPLVMQLTEPV
                     ATLSNSAGATLAGGAAHGEIHCALTLAWLAGCASLLFMRLRRRREFVRALGAGSEVYA
                     GREFDAMERARRRLGLERDARLMLSSHGAEPGVWRTRTPVVVLPETLSAHLDEEELES
                     LMLHELSHVERRDNLIGSLQTTLTCVFWFCPPVWLVSRRLLEERERACDERVLEAGGA
                     SAPYASSILKVVRFCSGWKVAGVSGAAAGPNLRRRIEDIMEARTTPERTAWQRTLPLA
                     AAVAALALSAGAGVYSYGQSGGGVRGAAARQQAQAGGGAKGGARVRVVPLNEAEGVAA
                     QEIEQTPEASVRFDNAGDAPLVITEARMRMVTREQLKRGADEGGEGHGFFLTMPSVTV
                     ANFTAKTVK"
BASE COUNT          579 a         1284 c         1443 g          634 t
ORIGIN      
        1 aagggggcgg ggcgccaccg ggcggggcgc gcccctcccc ttttcagcgg cggaaaccgg
       61 cgtcccgctt caggccgtga gcggcttgac gcgcttttcc gcctcggccc atacggcgtc
      121 gaggctattg ccgttcctct tgaaaatctt gagacactcg gagggggcca tgtcggtctg
      181 cgggagaagc tggatgtgcg gcgtgtcgac gatgccgctg aagcgcgcgc cccagaagag
      241 gccgagaccc tcggcctctt ctccggcggc gtcctgtatg gcgttgaagg tgcgccggaa
      301 ctccaccgac gaacccttcg gcacgtccgt gaagaccttg ccgccgatga ccgggtacat
      361 atccacggcg aggccgtagt tgtggttcga ctgacccccg cgcgccttcg tgactatgtt
      421 gccgggcttg gtgcgaccct gcgcgaagag cgcgtcctgc tcggcgaacg tgcgcaggcc
      481 gtcggtgatg agcgtctgca tcccgcgccg cgccagcgcc tccgcgagca gtacgagccg
      541 ctcccggaag acggggttga gcttcttcgt cttgccgcgc ttgtccatcg ccttaaaggc
      601 ttccgattct gtagagatga ccgtggggat gacggtcgtc acggtcgtca cggtcggcgt
      661 cgtgggctgg gtgccggtgc ccgtctccgt cgggttcgtg gcggcgggct ccgtcacgag
      721 cgatgcgggc acgttgacca cctgcccgat cttgatcttg tttatgtttt cgatctgcgg
      781 gttggcgctc tggagggcct ggagggtgac gccgtgctgc ttggcgatgc tggtcagggt
      841 gtcgccgggt ttgaccttgt gcttgatgag ggcgccgctc gtgggcttgg ggttgctgct
      901 catttcctta cggctcctta ttgtctaggg gatgtgaaag ctgctggtat ttgtaaagcc
      961 ggtcggtcgg ggacggcgcg cgggcttcct ctgttgcgcg cctgctggag ttgaaagttt
     1021 tagccgccgc ggacgtggcc ttccgcgagc gccgaagagg cgagggggtt tcgctcccct
     1081 ggggacagtt cacggagcac gcattatgag acaccggtca tggcaggtca acacttttag
     1141 cgacccagcg ttcggccaaa cgcggattga gacaacctcg tcggcgagac tataatctcg
     1201 gatgtttccg ggcgcggctg ttggcttcga cgctcatccc gatcacgaac ccaggtcgcg
     1261 cgtcgcacgg ccattccatt cggacggaac gaactgctgt gaaacggtca aacatgaagc
     1321 cactcgccgc ggccttcttc gcgctcgccg tgctcgccgc cgcgcacgtc ccgcccgccg
     1381 aggccgccac gcatcgccca ctcgatccgt ccgcagactt ccagaagcgc acggcgcggc
     1441 gcaagtccgc gcgccgcgcg ccccgccgcc ccgccgcccc cgccgacgcg aagcagcggc
     1501 ccgcgccgac gagcgcgacg accgagaagg ccgagacaac ggcgccgcag caccaggtcg
     1561 ttacgggcga ttcgtccacc atcggcatga cgccgcagca ggcgatggcc gtcgccatcc
     1621 gcgaggcgct gcgcaagccg cgcgagggag agacgcgcgt gcgcggcgtc ctcacgctca
     1681 tcgagtgcgg cccgaggaac gccgccatct tccacgtccg cgccggcgcc gagaccctca
     1741 agctcgacgg cggcgacctg cgcggcatca gcttcacggc cttcacgccg cgcgaggcgg
     1801 gcagcgagat cggctgcggc ccgcgcaagc tcgacacccc tgtcgtcgcc acctaccgcc
     1861 ccacggcgga gggcggcgac ctcgtcgccg tcgaattcgt gcccgccagc ttcacgcttg
     1921 agaagtaaca ggcgccgcct cgctcggcca agcgactgac cgaccgccga ccgccgggta
     1981 gcctcgaata attcccctcc gacggcgccc cgaaaattcc tcttgacctt cctccgacag
     2041 ttgtagtaga ttcaacctca gtcgtagtat ttcgccgttc gggaggccgg gatgccgaag
     2101 gagccgcagg tcaggctgac gaggttcgag ctggaggtga tggaggcgct gtgggcgctg
     2161 gggagcgcgt ccgtgcggga ggtgcaggag cgcctcccgg cgcgcaagcg cccggcctac
     2221 acgaccgtcc agacgatcat ctaccggctc gaagagaagg gcgcgctgcg ccgcgtgaag
     2281 aaggtcggca acgcgcacgt cttcgagccg gtggtctcgc gcaaggcggc ggcgcggcgc
     2341 ctcgtggacg agctgctcga cttcttcggc ggcagcccgc gcacgctgat ggcgcagatg
     2401 gtcgaggggg gccgcctgac gctcgaagac ctgcgcgagg ccgaaggcgc gctcgaacgg
     2461 atcgaggccg agcgcggcac ggcgggcgcg gcggaagaga aaggcgtcgg cacccctgcg
     2521 agcggggcgc gcagagatgc tgcgcgagag aggagaaaga gatgagcggg ccgcatgaat
     2581 ttctggcgcg cgcctccgcg tggctgtggc ctgcgctggt tgaccacctg tggcagtcgg
     2641 cgttgttcgc ggcgctcgtc ttcgcggcga cgctgctcgt gcggcgcggc ccggcgcgcg
     2701 cgcgccacgc gctgtggctg gtcgcgctcg tgaagttcgc ggtgccgccc gcgctcttcg
     2761 ccttcctcgc ggcgcgcgcg gggttggacg cgtcgtggct gttcgaaaac caatcggcgg
     2821 cggcgacaga gtacgcgccg ctcgtcatgc agttgacaga gccggtggcg acgctctcga
     2881 actctgccgg cgcgacgttg gccggcggag cggcgcacgg cgaaatccat tgcgcgttga
     2941 cgctggcatg gctcgcgggc tgcgcgtctc tgctgttcat gcgtctgcgg cggcggcgcg
     3001 agttcgtgcg cgcgctcggc gcaggaagcg aggtgtacgc gggccgcgag ttcgacgcga
     3061 tggagagagc gcgccgcagg ctcggcctcg aacgggacgc gcgtttgatg ctctcctcgc
     3121 acggcgcgga gccgggcgtg tggcgcacgc gcacgcccgt cgtcgtgctg cccgagacgc
     3181 tgtcggcgca cctcgacgaa gaggagcttg agtcgctgat gctccacgag ctgtcgcacg
     3241 tcgagcggcg cgacaacctg atcgggagcc tgcaaacgac gctgacgtgc gtcttctggt
     3301 tctgcccgcc cgtgtggctc gtcagccgaa ggctgctcga agagcgcgag cgcgcgtgcg
     3361 acgagcgcgt gctcgaagcg ggcggcgcgt ccgcgccata cgcgtcgagc atcttgaagg
     3421 tcgtgcgctt ctgctccggc tggaaggtgg ccggggtgtc gggcgcggct gccggcccta
     3481 acctgcgaag gaggattgaa gacatcatgg aggcacggac aacacctgaa cggacggcgt
     3541 ggcagcgcac gctgccgctc gcggcggcag tggctgcgct cgcgctctcc gcgggcgcgg
     3601 gggtctacag ttacgggcag agcggcggag gcgtgcgcgg cgcggcggcg cggcagcagg
     3661 cgcaggccgg cggcggcgcg aagggcgggg cgcgcgtgcg cgtcgtgccg ttgaacgaag
     3721 cggaaggggt cgccgcccaa gagatcgagc agacgccgga ggcatcggtt cgcttcgaca
     3781 acgcgggcga cgcgccgttg gtcatcaccg aggcgaggat gcggatggtg acgcgcgagc
     3841 agttgaagcg cggggccgac gagggcgggg aggggcacgg cttcttcctc acgatgccga
     3901 gcgtgacggt ggcgaacttc accgcgaaga cggtgaagga
//
LOCUS       DBNGOH010000760         3372 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2313088, whole genome shotgun sequence.
ACCESSION   DBNGOH010000760 DBNGOH010000000
VERSION     DBNGOH010000760.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3372)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3372)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3372
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2313088"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1146
                     /locus_tag="AC159P_14645"
     CDS             <1..1146
                     /locus_tag="AC159P_14645"
                     /inference="COORDINATES: protein motif:HMM:NF016289.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HIR3696737.1"
                     /translation="ASHGGRPLTVASYTWTPSTAGAVEGEVVDVGQGRPEDLARVRAR
                     LKDSVALVVFEGETLDALIYKFYRTPVLVGELKQAGAVAVLIASDKQHAMLYTSPVDF
                     TATGRMAALPTLSLGREDTALVRRLTGQGTAPRVRLDVRNRTGPAFESTNVVGEIRGR
                     ERPEEIVVVGAHLDSNDLGPGALDNAAGAAAVVEAARAIKALGLQPRRTIRFVLFMGE
                     EEGMVGSVAYVRRHRDELDRTVAALIMDIGAGRPLGWFSMGRTDLDTRIDELMLPLQQ
                     FGAGLIEHAAFAATDNAPFMAEGVPNLIMLQDESAYLPVHHTIGDTPDKVEPRDYAAA
                     VATLAVAAYSIADHPQRFGRRLTPEEVKKMADETKVADQWRAAGIWQ"
     gene            complement(1246..1536)
                     /locus_tag="AC159P_14650"
     CDS             complement(1246..1536)
                     /locus_tag="AC159P_14650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696738.1"
                     /translation="MSFWESVTRIIEKQFVDRAFNLSGQHKDFYCETCRRITDHVSIS
                     HAASMKRINGKQSRPLRMFEHVVGKLSDLNPAENILVGRPYRCGSCGTEITD"
     gene            complement(1581..1796)
                     /locus_tag="AC159P_14655"
     CDS             complement(1581..1796)
                     /locus_tag="AC159P_14655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696739.1"
                     /translation="MPVIGRLGGQVEEVLITPLERLRRQDAGEPPPEDEPARGDATDS
                     RGGHGERAARDEVGNDDVRDELPVWLL"
     gene            1877..3166
                     /locus_tag="AC159P_14660"
     CDS             1877..3166
                     /locus_tag="AC159P_14660"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HIR3696740.1"
                     /translation="MSNIPASLRRASQRTLSLVIFLPALLAHGLQPATGQPTRAAATA
                     AQRQEQQRPRRVAPTPTPESGAGRGERPASAPQKSPGGGEAVDEDDVVRIDTQLVSVP
                     AVVTDGRGKPVSGLRADNFTLLEDGRPQKIANFATTEAPFEVALLLDTSGSTRAEVGL
                     IRRAAGVFVESLRPGDRVAILAFSTREEGDTKLAEVEVKSPLTDDREQLREAIEGIGA
                     SYGTPFYDGLERVAAEVFRDAPRAELRGRRALVALTDGVDSTSESDYEEARARFKRAG
                     VVAYFVQVNTEAFVEERLMRDCQDDGALRLSRSQLQRYRRLFAARADAADYADFCRMG
                     QFERMDISRRLYQLARAEMNELARDSGGKTFPVGDLREARGAFRQIAEEIGTQYSLGY
                     YPTNKARDGSFRTIRVQVRGVRDAQVRAREGYQAPRS"
     gene            3224..>3372
                     /locus_tag="AC159P_14665"
     CDS             3224..>3372
                     /locus_tag="AC159P_14665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011316800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5996 family protein"
                     /protein_id="HIR3696741.1"
                     /translation="MAQSNSTQDAAEVWPSLPLAEWRETYATLHMWTQIVGKVRLAQT
                     PLVNH"
BASE COUNT          547 a         1181 c         1145 g          499 t
ORIGIN      
        1 gcctcgcacg gcggacgccc gctcacggtc gcttcgtaca cgtggacgcc ttcgacggcg
       61 ggcgccgtcg aaggcgaggt cgtggacgtg gggcagggaa ggccggaaga cctcgcacgc
      121 gtccgcgcgc gattgaagga cagcgtcgcg ctcgtcgtct tcgaaggcga gacgctcgac
      181 gctctcatct acaagttcta ccggacgccc gtgctcgtcg gcgagttgaa gcaggcgggt
      241 gcggtggccg tgctcatcgc gtcggacaag cagcacgcga tgctctacac gtcgccggtg
      301 gacttcacgg cgaccggaag gatggcggcg ctgccgaccc tctcgctcgg gcgcgaggac
      361 acggcgctcg tgcggcggct cacagggcag ggcaccgcgc cgcgcgtgcg gctcgacgtg
      421 cggaacagga cggggcctgc gttcgagagc acgaacgtgg tcggcgaaat ccgcgggcgc
      481 gagcggcccg aagagatcgt cgtcgtgggc gcgcacctcg actcgaacga cctcgggccg
      541 ggcgcgctcg acaacgcggc gggcgccgcg gcggtcgtcg aagccgcgcg cgccatcaag
      601 gcgctcggcc ttcagccgcg gcgcacgatc cgcttcgtgc tcttcatggg cgaagaggaa
      661 gggatggtcg gctcggtggc ctacgtccgc cgccaccgcg acgagctgga cagaacggtc
      721 gcggccctca tcatggacat cggcgccggg cgcccgctcg gctggttctc gatgggccgc
      781 accgacctcg acacgcggat cgacgaactg atgctgccgc tccaacagtt cggcgctggt
      841 ctcatcgagc acgcggcctt cgccgccacc gacaacgcgc ccttcatggc cgagggcgtc
      901 ccgaacctca tcatgcttca ggacgagtcg gcctacctgc ccgtccacca caccatcggc
      961 gacacgcccg acaaggtcga gccgcgcgac tacgccgccg cggtcgccac gctcgccgtc
     1021 gccgcctact ccatcgccga tcacccccaa cgcttcggtc gccgcctgac gccggaagaa
     1081 gtcaaaaaga tggccgacga gacgaaggtc gcagaccagt ggcgagccgc gggcatctgg
     1141 caataagaag aaggggaaaa ggggaagagg ggaaagggcg aaaaggggaa gtcatgattt
     1201 tcccttttcg ccctttcccc ttttaacctt ttcgcccttc ttcccctagt cggtgatttc
     1261 ggtgccgcaa ctgccgcagc ggtaggggcg accgacgagg atgttctcgg cggggttgag
     1321 gtcgctgagc ttgccgacga cgtgttcgaa catgcggagg ggccgcgact gcttgccgtt
     1381 gatgcgcttc atgctggcgg catggctgat ggagacgtgg tccgtgatgc ggcggcaggt
     1441 ctcgcagtag aagtccttgt gctggccgct gaggttgaag gcgcggtcaa cgaactgctt
     1501 ttcgatgatg cgcgtcacgc tctcccaaaa actcatgatc gcagaccctt gagggtgtag
     1561 aaacgcggga gcgccgtgct tcaaagaagc cacaccggca gctcgtcccg cacgtcgtcg
     1621 ttcccgacct cgtcccgcgc ggcacgctcc ccgtggccgc cgcgactgtc ggtcgcatcg
     1681 ccgcgcgccg gctcgtcttc gggcggcggt tcgcccgcgt cctgccgccg aagcctttcg
     1741 agcggcgtga taagcacctc ttcgacctga ccgccgagtc tcccgatgac gggcatgact
     1801 tgtcgtccgc ccctttccgt ccgcgcgcgt ttgcggctat atttgaccgt gaatttcctg
     1861 cggagagtgt aagcccgtgt cgaatattcc ggcaagcttg aggcgcgcgt cgcaacgcac
     1921 gctgtccctc gtcatttttc tcccggcgct gctcgcgcac ggtctacagc ccgcgaccgg
     1981 ccagccgacg cgggccgcag cgaccgccgc gcaacgacaa gagcagcagc gcccgcgccg
     2041 cgtcgcgccc acgccgacgc ctgaaagcgg cgcgggcagg ggcgagcgcc ccgcgtcggc
     2101 gccgcagaag agtccgggcg gcggcgaggc cgtggacgaa gacgatgtgg tgcgcatcga
     2161 cacgcagctc gtctccgtcc cggcggtcgt gacggacggg cgcgggaagc ccgtctcggg
     2221 cctgcgcgcc gacaacttca cgctcctcga agacgggcgc ccgcagaaga tcgccaactt
     2281 cgccacgacc gaggcgccgt tcgaggtcgc gctgctgctc gacacctccg gctcgacgcg
     2341 cgccgaggtc ggcctcatcc gccgcgccgc gggcgtcttc gtcgaatcgc tccgccccgg
     2401 cgaccgcgtc gccatcctcg ccttcagcac gcgggaggag ggcgacacga agctcgccga
     2461 ggtcgaggtc aagtcgccgc tcacggacga ccgcgagcag ttgcgcgagg ccatcgaagg
     2521 catcggcgcc agctacggca cgcccttcta cgacggcctt gagcgcgtcg cggcggaggt
     2581 cttccgcgac gccccgcgcg ccgagctgcg gggccgccgc gccctcgtcg ccctcacgga
     2641 cggcgtggac tcgacgagcg agagcgacta cgaagaggcg cgcgcgcgct tcaagcgcgc
     2701 cggggtggtc gcttacttcg tgcaggtcaa cacggaggcg ttcgtcgaag agcgactgat
     2761 gcgcgactgc caggacgacg gtgcgctgcg cctctcgcgc tcgcagctcc agcgctaccg
     2821 ccgcctcttc gccgcgcgcg ccgacgccgc cgactacgcc gacttctgcc gcatgggcca
     2881 gttcgagcgc atggacatca gccgccgcct ctaccagctc gcgcgcgcgg agatgaacga
     2941 gctggcgcgc gactccggcg gcaaaacctt ccccgtcggc gacctgcgcg aggcccgcgg
     3001 cgccttccgc cagatcgccg aagagatcgg cacgcagtac agcctcggct actacccgac
     3061 caacaaggcg cgcgacggct ccttccgcac catccgcgtt caggtgcgcg gcgtccgcga
     3121 cgcgcaggtg cgcgcccgcg aaggctacca ggcgccgcga agttagaggg cgacgaaaag
     3181 tagacgctgc aagtttgcag gacgaggggt gagccgcaac gagatggccc aatcgaactc
     3241 aacgcaagac gccgccgaag tttggccgag cctgccgctg gccgagtggc gcgagactta
     3301 tgcgacgctg cacatgtgga cgcagatcgt cggtaaagtc cggctcgcgc agacgccgct
     3361 ggtcaaccat tg
//
LOCUS       DBNGOH010000761         3936 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4145519, whole genome shotgun sequence.
ACCESSION   DBNGOH010000761 DBNGOH010000000
VERSION     DBNGOH010000761.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3936)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3936)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3936
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4145519"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            103..1512
                     /gene="cysS"
                     /locus_tag="AC159P_14670"
     CDS             103..1512
                     /gene="cysS"
                     /locus_tag="AC159P_14670"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HIR3696742.1"
                     /translation="MLKFFNTLTNRLEEFRPMREGEVRLYLCGPTVWDYAHIGNFRTF
                     AAFGDTLRRYLKYKGYRVTHVMNVTDIEDRIINFSREQRVTIDEYTARYTEAFFDDLA
                     ALGAERPEETPRATRHIPEMVEIIKRLVERGHAYESEGSYYYRISSFPEYGKLSKINF
                     AGNIAGASERVDTDKYDKEDARDFALWKAKDSPDEPSWQTELGDGRPGWHIECSAMSM
                     KYLGETFDIHAGGIDLVFPHHENEIAQSEGATGRKFANYWLHAEHLKVEGESMSKSKG
                     NYYTFRDLRDKGFHPSAIRYLLLSVPYRKQLNFTLEGLRGAAKTVESLRDFRARLKNA
                     RTEPGANPELKAAAARALEEFEAGMDDDLNTSVALAAVHNLSREVNRALDQHAVREDD
                     RRDILDLVARIDSVLNIFGCDEEVRLEEEIQSLINERQQARHRRDFARADEIRDCLAE
                     RGIVLEDTRDGVRWKRRGT"
     gene            1799..3154
                     /locus_tag="AC159P_14675"
     CDS             1799..3154
                     /locus_tag="AC159P_14675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014098758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl ornithine aminotransferase family
                     protein"
                     /protein_id="HIR3696743.1"
                     /translation="MSATAITKRPEIKTATLPGPKGQLIVEADAQWVTPSYPRPSFKL
                     VAERGEGVWVEDVDGNVFLDCNAGVAVCSTGHCHPEVVKAIQEQAAQLIHMCGTDYYY
                     RHMPELAEVLDRIVPVPSPTRTHFANSGTEAVETALKLAIHATGREKFIAFFNSFHGR
                     TLGSLSLTSSKAAQRRGFKRQALDVVHVPYPNQFRHPFTGEKSDAETVSRDCLNWIEQ
                     RLFKTTTPPEEVAGIVVEVVQGEGGYVPAPKEFLQGLRRICDREGILLIVDEVQSGMG
                     RTGKMFACDHYDLKPDIITLAKGIASGLPMGACVARADLMDWKPGAHASTFGGNPVAL
                     ASALKTIELLERELVANSAEVGAYLQAGLRKLMAKHDCVGDVRGLGLMVGVEFVEAGT
                     GAPAPALRDRVEIECFNRGLIILGAGFNTIRWSPPLVLSKENVDVALELFDEAITAAR
                     A"
     gene            complement(3270..3752)
                     /locus_tag="AC159P_14680"
     CDS             complement(3270..3752)
                     /locus_tag="AC159P_14680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006748740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding protein"
                     /protein_id="HIR3696744.1"
                     /translation="MRKRIISTTTRPAPEAGADWLPVEELATIEVTSEDPSHPIEHAL
                     LRGGEADEPGWRAAVPGEQTVRLVFDRPQQLKRVRLLFVERERERSQEFVLRWSPDGG
                     HTFRDIVRQQWNFSPHGSTRETEDYRAELSGVTQLELQIVPDRTGGDARAALAQLRLQ
                     "
BASE COUNT          676 a         1369 c         1243 g          648 t
ORIGIN      
        1 atgctcagtc cggcgtgatg tttgactcgc cgccgctcct tgcctagact cttcggcacg
       61 cgccgtccct cgcctgagag agaggccggg cggttgttca cgatgctcaa atttttcaac
      121 acgctcacga accggctcga agagttccgc ccgatgcgcg agggcgaggt caggctctat
      181 ctctgcggcc cgaccgtgtg ggactacgcg cacatcggca acttccgcac cttcgccgcc
      241 ttcggcgaca cgctgcgccg ctacctcaag tacaagggct accgcgtgac gcacgtgatg
      301 aacgtcacgg acatcgagga tcgcatcatc aacttctcgc gcgagcagcg cgtgacgatt
      361 gacgagtaca cggcccgcta caccgaagcc ttcttcgacg acctcgccgc cctcggcgcc
      421 gagcggcccg aagagacgcc ccgcgccacg cgccacatcc cggagatggt cgagatcatc
      481 aagcggctcg tcgagcgcgg ccacgcctac gagtccgaag gctcgtacta ctaccgcatc
      541 agctcgttcc ccgagtacgg caagctctcg aagatcaatt tcgcgggcaa catcgccggc
      601 gcgagcgagc gcgtggacac ggacaagtac gacaaggagg acgcgcgcga cttcgcgctg
      661 tggaaggcga aggactcgcc ggacgagcct tcgtggcaga cggagctggg cgacgggcgg
      721 cccggctggc acatcgagtg ctcggcgatg tcgatgaagt acctcggcga gacgttcgac
      781 atccacgcgg gcggcatcga cctcgtcttc ccgcaccacg agaacgagat cgcgcagagc
      841 gagggcgcga cgggtcggaa gttcgccaac tactggctcc acgccgagca cctcaaggtc
      901 gaaggcgaga gcatgtcgaa gtcgaagggc aactactaca ccttccgcga cctgcgcgac
      961 aagggcttcc acccgtcggc catccgctat ctgctgctga gcgtgcccta ccgcaagcag
     1021 ctcaacttca cgctcgaagg cttgcgcggc gcggccaaga cggtcgagtc cctgcgcgac
     1081 ttccgcgcgc gcctgaagaa cgcgcgcacc gagccgggcg cgaaccccga gctgaaggcc
     1141 gccgccgcgc gcgcgctcga agagttcgag gccgggatgg acgacgacct caacacctcg
     1201 gtcgcgctcg ccgccgtcca taacctctcg cgcgaggtca accgcgcgct cgaccagcac
     1261 gccgtccgcg aggacgaccg gcgcgacatc ctcgatctcg tggcgcgcat cgactccgtg
     1321 ctcaacatct tcggctgcga cgaagaggtg cggctcgaag aggagataca gtcgctcatc
     1381 aacgagcgcc agcaggcacg ccaccgccgc gacttcgccc gcgccgacga gatccgcgac
     1441 tgcctcgccg agcgcggcat cgtcctcgaa gacacgcgcg acggcgtccg ctggaagcgg
     1501 cgcggaacct aatgctaaag taacgaggac tgaggactga ggactgagtg gaagcattaa
     1561 cgattgatga gaggcgtgct ggacggactc gtcgcgggtt acgggtcacg ccttccactc
     1621 agtcctcagt cctcagtcct cagtactgag ttatgtttat tccttcgacc ctccactttc
     1681 gaccgctcga cagccgcgcc gatctcttcg cggactagcc acgcgtctct ccaacccgcg
     1741 cgccttcaat tcctcgtcgg aagactcaac ccctcacttt caccaaagga gagagatcat
     1801 gtctgctacc gcgataacga agaggccgga gatcaagacc gcgacgctgc ccggcccgaa
     1861 gggccagctc atcgtcgaag ccgacgcgca gtgggtcacc ccctcttacc cgcgcccgtc
     1921 ctttaagctc gtcgccgagc gcggcgaggg cgtctgggtc gaggacgtgg acggcaacgt
     1981 cttcctcgac tgcaacgcgg gcgtcgccgt ctgctcgacg ggccactgcc acccggaggt
     2041 ggtcaaggcc attcaggagc aggccgcgca gctcatccac atgtgcggca ccgattatta
     2101 ctaccgccac atgccggagc tggccgaggt cttggatcgc atcgtgcccg tccccagccc
     2161 gacgcggacg cacttcgcca acagcgggac ggaggccgtc gagacggccc tcaagctcgc
     2221 gattcacgcc accgggcgcg agaagttcat cgccttcttc aacagcttcc acgggcgcac
     2281 gctcggctcg ctctcgctca cctcttcgaa ggccgcgcag cgccgcggct tcaagcgtca
     2341 ggcgctcgac gtggttcacg tcccttaccc gaaccagttc cgccacccgt tcaccggcga
     2401 gaaatccgac gccgagacgg tcagccgcga ctgtctgaac tggatcgagc agcggctctt
     2461 caagacgacc acgccgcccg aagaggtcgc cggcatcgtc gtcgaggtgg tgcagggcga
     2521 gggcggctac gtccctgcgc cgaaagagtt cctacagggc ttgcgccgca tctgcgaccg
     2581 ggaaggcatc cttttgattg ttgacgaggt gcagtcgggg atgggccgca cgggcaagat
     2641 gttcgcgtgc gaccactacg atttgaagcc cgacatcatc acgctggcga agggcatcgc
     2701 gtcgggcctc ccgatgggcg cgtgcgtggc gcgcgccgat ttgatggact ggaagccggg
     2761 cgcgcacgcc tctacgttcg gcggcaaccc ggtcgccctc gcctctgcgc tgaagaccat
     2821 cgagctgctc gaacgcgagc tggtcgccaa cagcgcggag gtcggcgcct atctacaggc
     2881 gggcctgcgg aagctgatgg cgaagcacga ctgcgtcggc gacgtacgcg gcctcggcct
     2941 catggtcggc gtcgagttcg tcgaggccgg cacgggcgcg cctgcgcctg ccttgcgcga
     3001 ccgcgtcgag atcgagtgct tcaaccgcgg cctcatcatc ctcggcgccg gcttcaacac
     3061 catccgctgg tcgccgccgc tcgtcctctc gaaagagaac gttgacgtgg cgcttgagct
     3121 tttcgacgag gccatcaccg ccgcacgggc ctgagcagta gcagtaggca gtcggcggtg
     3181 ggccttcaag acaagagcaa gaggcttggg agattcactc ccaagcctct tgcttttgtc
     3241 ttgactacct accgcttact gcctaccgcc tactgtaatc gcaattgcgc gagcgcggcg
     3301 cgggcgtcgc cgccggtgcg gtcggggacg atttgcagtt cgagctgggt gacgccggag
     3361 agttcggcgc ggtaatcttc cgtctcgcgc gtggagccgt gggggctgaa gttccactgc
     3421 tggcggacga tgtctcggaa ggtgtgcccg ccgtcgggcg accagcgcag gacgaattcc
     3481 tgcgagcgct ctcgctcgcg ctcgacgaag agcagccgca cgcgcttgag ctgttgcggg
     3541 cggtcgaaga cgaggcgcac ggtctgttcg cccggcaccg ccgcgcgcca gcccggctcg
     3601 tccgcttcgc cgccgcggag cagcgcgtgc tctatcgggt gcgaagggtc ttcggaggtg
     3661 acttcgatgg tcgccaactc ttcgaccggc agccagtcgg cgcccgcttc cggcgcgggc
     3721 cgcgtggtcg tgctgatgat gcgtttacgc atggcccgcc ctccgtctcg tcgtgtgccg
     3781 acaagctccc tgccccgccc cgtccggcgg cccgctcagc ccctgcactg cgggcactga
     3841 cattcgtcac tcctgttgcg tcccactccc atccgcagtt cgccgcacgc cgcacgcgcc
     3901 ggcgagagcg tctctcccct gcgccgtctc ggtgcg
//
LOCUS       DBNGOH010000762         3262 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4512987, whole genome shotgun sequence.
ACCESSION   DBNGOH010000762 DBNGOH010000000
VERSION     DBNGOH010000762.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3262)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3262)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3262
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4512987"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1944)
                     /locus_tag="AC159P_14685"
     CDS             complement(<1..1944)
                     /locus_tag="AC159P_14685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TolB-like translocation protein"
                     /protein_id="HIR3696745.1"
                     /translation="MKHAFRPSRLGAAALMLAFLVGAFVSSSRVPASRAQTSALSPTP
                     DHFVAQITRTTIPFIPASTPTPTATPTPTPTPTATPTPGAPINLSSFATSMSGDGRLV
                     VIESEGDIATPPPGVPKEEMVPNNRDGNLEIFLFDYAQRRIFQLTDTRHALKDAAATP
                     IERQNIDVFVSNHRATISRDGRYVVFASNAYSDAAPGVTPKSFNGQNHVAALKADANS
                     EIFYYPIPVLPAADLSSGEEVPFTNLANETPVRLTFTPATALARPGTANAAPFFADDN
                     RPNDINDDGSYIAFTTEAKSGLTGVANADGNPEVVIVRRTTGAFTQITETADLPPTPT
                     SPLGTLVFNDNPTISGSNTTARVAFLSNADINSDAAEADRRNGEIYVAAYDGTARASQ
                     TCRNVMTTDVCPVTKTPPERRIGFEGVSVNVFNPGRRISRDGNYLVFESTAVLNRDGT
                     LNGALATTYGIYLYHRAANQFTQVANRVPDTESADLPNRFPTFTLDSTRVVWSSTINY
                     RADGTVAAAGSNEGLNPLRGTQLFTVPVEQLIAPTPVNQVSRLTNQNIISPSQVHPSN
                     TFRRIAFNLFGAFQYGEGNPDVLLSEALYLVVPAVTSETPAPSPSPAASPAPVSFFTG
                     ASERPVVAASPAPSPSPGPAVLSL"
     gene            complement(2289..>3262)
                     /locus_tag="AC159P_14690"
     CDS             complement(2289..>3262)
                     /locus_tag="AC159P_14690"
                     /inference="COORDINATES: protein motif:HMM:NF015140.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4214 domain-containing protein"
                     /protein_id="HIR3696746.1"
                     /translation="LKDFNAALGTLRFAPGETEKIVTVFVTNDRYVESNEDFRVTLSN
                     PVGCALGGATGATVTIMSDDVVNGPNPVDWSASFDPAFFVRQQYIDFLGREPDADGLA
                     FWSAGIINCGADAACAEPQRVNVSAAFYLSIEFQETGFLAYRMHKAAYGNLPGASVPV
                     RIGEFLPDQRRIGEGVQVGIGPWQQQLEANKAAYAADFAARAPFTSRYPSTISPAQFV
                     DALNLNAGGALSQAERDQLVNSLTGGTMTRAEVLRRVAEDADFAAAEKSRAFVMMQYM
                     GYLRRDPDEGGYLHWLQKLNDFGGDFNRAEMVKAFITSIEYKERFGP"
BASE COUNT          488 a          954 c         1292 g          528 t
ORIGIN      
        1 gagactcaag accgccgggc cgggcgaggg cgagggtgcc gggctggcgg cgaccacggg
       61 ccgctcgctc gcgcccgtga agaacgagac gggcgcgggg ctggccgcgg gcgacgggct
      121 gggcgcgggc gtctcgctcg tcaccgcggg cacgacgagg tagagcgcct cggagaggag
      181 cacgtccggg ttgccctcgc cgtactggaa ggcgccgaag aggttgaagg cgatgcgccg
      241 gaacgtgttg ctcggatgca cttgcgacgg cgagatgatg ttctggttcg tcaggcgcga
      301 gacctgattg acgggcgtcg gggcgatgag ctgctcgacg ggcacggtga aaagctgcgt
      361 gccgcgcaag gggttcagcc cctcgttcga gccggcggcg gcgaccgtgc cgtcggccct
      421 gtagttgatg gtcgaagacc agacgacgcg cgtgctgtcg agcgtgaagg tcgggaagcg
      481 gttcggcaaa tccgcgctct cggtgtcggg cacgcggttg gcgacctgcg tgaactggtt
      541 cgcggcgcgg tggtagaggt agatgccgta ggtcgtcgcc agcgcgccgt tgagcgtgcc
      601 gtcgcggttg aggacggcgg tgctctcgaa gacgaggtag ttgccgtcgc ggctgatgcg
      661 ccggcccggg ttgaagacgt tgacgctgac gccctcgaag ccgatgcggc gctcgggcgg
      721 ggtcttcgtg acggggcaca cgtcggtggt catgacgttc cggcacgtct gcgaggcgcg
      781 cgccgtgccg tcgtaggcgg cgacgtagat ttcgccgttg cggcggtcgg cctcggccgc
      841 gtccgagttg atgtcggcgt tcgagaggaa ggcgacgcgg gccgtcgtgt tgctgccgga
      901 gatggtcggg ttgtcgttga agacgagcgt gccgagcggg ctggtcggcg tcgggggcag
      961 gtcagccgtc tcggtgatct gcgtgaaggc gccggtggtg cgtctgacga tgacgacctc
     1021 ggggttgccg tcggcgttcg ccacgcccgt caggcccgac ttggcctcgg tggtgaaggc
     1081 gatgtaggag ccgtcgtcgt tgatgtcgtt ggggcggttg tcgtcggcga agaagggcgc
     1141 ggcgttggcc gtgccggggc gcgcgagcgc ggtcgcgggc gtgaaggtga gccgcacggg
     1201 cgtctcgttg gcgaggttcg tgaacggcac ctcttcgccc gacgagaggt ctgccgcggg
     1261 cagcacgggg atcgggtagt agaagatttc ggagttcgcg tcggccttga gcgcggcgac
     1321 gtggttctgg ccgttgaagc tcttcggcgt cacgccgggg gcggcgtcgc tgtaggcgtt
     1381 cgaggcgaag acgacgtagc gcccgtcgcg cgagatcgtg gcgcggtggt tcgagacgaa
     1441 cacgtcgatg ttctgccgct cgatgggcgt cgcggcggcg tccttgagcg cgtgccgggt
     1501 gtcggtgagc tggaagatgc ggcgctgcgc gtagtcgaag aggaagattt ccaggttgcc
     1561 gtcgcggttg ttgggcacca tctcctcttt gggcacgccg ggcgggggcg tggcgatgtc
     1621 gccctcggac tcgatgacca cgaggcgccc gtcgccgctc atgctggtgg cgaaggagct
     1681 gaggttgatg ggcgcgccgg gcgtcggggt cgcggtcggc gtcggcgtcg gggtcggggt
     1741 ggcggtcggc gtgggcgtcg aggccgggat gaacgggatc gtcgtccggg tgatctgcgc
     1801 gacgaagtgg tcgggcgtgg gcgagagcgc cgaggtctgc gcgcgcgacg cggggacgcg
     1861 cgacgaggag acgaaggcgc cgacgaggaa cgccagcatc agcgcagccg cgccgaggcg
     1921 ggacggacga aaagcgtgct tcacaaaaaa ccctccgaac gggtgcataa gaaccgttga
     1981 cgaataaagc ccacgggaga cactgaaaag cgggcggtaa tcaggcgcgc gcccgccgcc
     2041 gctctgggaa gggcgtcggg cgcgccgatg gagtcaaaga aaaggcattt taagcgtaag
     2101 gctcgcgcga gcgcaagcag agcgccgggc cggcggaagg cgtcccgcgg cgagcctgcc
     2161 tgttcggatg actgagatgg ttttgccgcc gccccgcgct gccgtggccg gaagcccccg
     2221 cgcgcggggc gcgaagaaaa tacgagtcgc gcacgtttac gattaacgtc gcggggcaaa
     2281 gcgttgcctc aaggcccgaa acgctctttg tattcgatgg acgtgatgaa cgccttgacc
     2341 atctcggcgc ggttgaagtc gccgccgaag tcgttgagct tctggagcca gtgcaggtag
     2401 ccgccctcgt ccgggtcgcg ccggaggtag cccatgtact gcatcatcac gaaggcgcgc
     2461 gacttctccg ccgccgcgaa gtccgcgtcc tcggccacgc ggcgcagaac ctctgcgcgc
     2521 gtcatcgtgc cgcccgtcag agagttgacg agctggtcgc gctcggcctg cgagagcgcg
     2581 ccgcccgcgt tcaggttcag cgcgtccacg aactgcgcgg ggctgatggt cgaagggtag
     2641 cgcgaggtga agggcgcgcg cgccgcgaag tccgcggcgt aggccgcctt gttcgcttcg
     2701 agctgctgct gccacgggcc gatgccgacc tgcacgccct cgccgatgcg ccgctggtcg
     2761 ggcaggaact cgccgatgcg cacaggcacg gaggcaccgg gcaggttgcc gtaggcggcc
     2821 ttgtgcatgc ggtaggccag aaagcccgtc tcctggaatt cgatggaaag atagaaggcc
     2881 gccgagacgt tgacgcgttg cggctcggcg caggcggcgt cggcgccgca gttgatgatg
     2941 cccgccgacc agaaggcgag gccgtcggcg tcaggctcgc gcccgaggaa gtcgatgtac
     3001 tgctggcgga cgaagaaggc ggggtcgaag ctcgcactcc aatcgacggg gttcgggccg
     3061 ttcacaacgt cgtcgctcat gatcgtcacc gtcgcgcccg tcgccccgcc gagcgcgcag
     3121 cccacggggt tgctgagcgt gacccggaag tcttcgttcg attcgacgta gcggtcgttg
     3181 gtgacgaaga cggtgacaat cttctcggtc tcgccgggcg cgaagcgcaa cgtgccgagc
     3241 gccgcgttga aatccttgag gt
//
LOCUS       DBNGOH010000763         9146 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_298132, whole genome shotgun sequence.
ACCESSION   DBNGOH010000763 DBNGOH010000000
VERSION     DBNGOH010000763.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 9146)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9146)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9146
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_298132"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..623
                     /locus_tag="AC159P_14695"
     CDS             <1..623
                     /locus_tag="AC159P_14695"
                     /inference="COORDINATES: protein motif:HMM:NF014646.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HIR3696747.1"
                     /translation="LDGSAEKASALLTDAHASGRGLAALFDEVLCPAMRRVGDLWHEG
                     ELTVAQEHVATRAALSALQTLGGALAEAEPFAPLAVCCGAEDDFHELPVNCSELVLHE
                     GGWAVLNLGANTPFYALAEAVARRGPRLVAVAATVLADLDRAVSEFKDLRAAAARAGA
                     AVVLGGAGFADAAVRRRLPADLHADSFMQLSELSEKKKNEVMRTED"
     gene            complement(741..1661)
                     /locus_tag="AC159P_14700"
     CDS             complement(741..1661)
                     /locus_tag="AC159P_14700"
                     /inference="COORDINATES: protein motif:HMM:NF013100.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HIR3696748.1"
                     /translation="MRHTKTILTAAILSAALLSAQACARADETSHVAAPQTPPSPRAV
                     TPSTTATPSTVVDKDATQPDLARHFEQFPGTFVLYDLKRDRYVRHDPARAAARFSPFS
                     TFKIPNSLIGLETGVIKDADFLIKWDAEKYPREGQFDFWWRDHTLRSAFRNSVVWYYR
                     ELASRVGAEKMREFVNKFRYGTQDISGGVDRFWLGSSLKISADEQVEFLKALYREELP
                     VSKRTYAVFKNIFVNEEGAGYKLSGKTGGGPFAEGRFLGWFVGYLETKDNAYVFATQI
                     EGPTFASIRDERLRITKSILTDLGHLPGKK"
     gene            complement(1943..2353)
                     /locus_tag="AC159P_14705"
     CDS             complement(1943..2353)
                     /locus_tag="AC159P_14705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003242464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696749.1"
                     /translation="MQSFPTSTFLRRVLLADAATCLAAGLLMTLGAGLLEQFFGLPSG
                     LSRYAGVGLLPFAAFLVYLATRETLPTAAVWAVIVLNVLWTLDSLLLLLSGWVAPTPP
                     GYAFVIAQALGVAMFAALEYVGLKKATTATTAAV"
     gene            2538..3344
                     /locus_tag="AC159P_14710"
     CDS             2538..3344
                     /locus_tag="AC159P_14710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014199358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmyB family transcriptional regulator"
                     /protein_id="HIR3696750.1"
                     /translation="MGEVSTRPVGTLLRDWRRRRRLSQLDLALEADISSRHLSFLETG
                     RSQPSREMVLRVAARLEVPLRGRNALLVAAGYAPVFKERPLEDPALGAARRAVDLVLA
                     GHEPFPALAIDRHWTLVAANRAVPLMLAGIDPAVLAPPVNVLRLSMHPRGLAPRIVNL
                     AEWRTHLLARLRRQIEATADAVLAELFEELSRYPVPDDGGHDAGAATDYAGVAIPLQL
                     ATEGGVLSFLSITTVFGTPVDITLSELALESFFPADAATSDALNRIMKRD"
     gene            complement(3369..4058)
                     /locus_tag="AC159P_14715"
     CDS             complement(3369..4058)
                     /locus_tag="AC159P_14715"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR3696751.1"
                     /translation="MTAHEPSTLRPTRAVLWDVDGTLIDSSEYHWLSWRDALAAENFP
                     LTREQFASIFGRRNDEILREYFGPGFPAAEVARVGDAKETLYRALVRERGITLLPGVR
                     RWLDKLRGEGWRQAMASSAPRANLEAITGALGIGDYFASIVSAEDVTRGKPDPQVFLT
                     AAARLEVAPSSCVVVEDAPSGVEAARRAGMRAVGVLTAHADLGADIVVRTLEELPDNA
                     FDELLGPRESP"
     gene            4208..4699
                     /locus_tag="AC159P_14720"
     CDS             4208..4699
                     /locus_tag="AC159P_14720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012567717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6789 family protein"
                     /protein_id="HIR3696752.1"
                     /translation="MNTVLLGALAGFAATAPMSLAMKLMHEQLPTHEQYPLPPREVAM
                     EVAEKAGIKHELDEPEREGLTWASHFAYGAASGAVYAPLARAVDLPPPLTGAAFGLAV
                     WAGSYLGWLPAAGVLRPATEHPPRRTALMIAAHVVWGVAAGVIIDQLSDGRRGPQGGR
                     RSR"
     gene            4696..6018
                     /locus_tag="AC159P_14725"
     CDS             4696..6018
                     /locus_tag="AC159P_14725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3696753.1"
                     /translation="MSDAVAGCLTSGAVAGDVAGGAMKTEDGAAKAEPSPVEAPPAGR
                     WRALVLLACAELLCMSLWFSASAVVPALQAEWRLTEAGASWLTLAVQLGFVAGTLLSA
                     LLNLPDIISTRRLVVAAAVCAAVVNAAFGLFAHDLTTGVVLRFLVGLFLAGVYPPGMK
                     IMATWFRRGRGAALGVLVGALTLGKAAPYLVNAVGSDEWRVNVLFASLLAAVGAFVVL
                     LFVGDGPYGTPPARFDIRQAARVFGNRGVRLANFGYFGHMWELYAMWTWLPVMMRAAL
                     AERGEPAALAEVASFLVIGAGALGCVAAGLVADRAGRTLVTSAAMVVSGACCLLVGFT
                     FEAGSTALLLAVAVVWGASVVADSAQFSACVTELGDPRYVGTALTIQTCCGFLLTTVS
                     IHLVPLLVRHIGWRYAFAALAPGPVFGVVSMLRLRALPEASRIAGGKR"
     gene            6089..6634
                     /locus_tag="AC159P_14730"
     CDS             6089..6634
                     /locus_tag="AC159P_14730"
                     /inference="COORDINATES: protein motif:HMM:NF012514.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HIR3696754.1"
                     /translation="MKKHGPWTVKSSERLYQDKYVEFRVDEVVKPSGEPSRYATGRML
                     PGVSVLALDAEGFVHLTKQFRYAVGRESIEVVSGGLDEGEEEGRAALRELREELGIEA
                     GELVDLGLVDAVTSQVSAPARIYLARELKFGEPDRGGSELMETLRVKLEEAVRMVMAG
                     DITHAISCVLILKTKEVLRTE"
     gene            complement(6714..7175)
                     /locus_tag="AC159P_14735"
     CDS             complement(6714..7175)
                     /locus_tag="AC159P_14735"
                     /inference="COORDINATES: protein motif:HMM:NF013936.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HIR3696755.1"
                     /translation="MNAFQLLKEDHKKVSAIFQQLEPTTERAVKTRSELFAELKTNLD
                     IHAHIEETIFYPSIKREAETREIVLEGFEEHHVIKMLLQELEGMPVDTEQWAAKLKVL
                     QENVEHHVEEEEGEMFQKARDVLSEEQIEQLGARMEEEKKRQEGQTKTAKA"
     gene            7425..>9146
                     /locus_tag="AC159P_14740"
     CDS             7425..>9146
                     /locus_tag="AC159P_14740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002638629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FdhF/YdeP family oxidoreductase"
                     /protein_id="HIR3696756.1"
                     /translation="MTNEEKVSEETASAAQPSHYNVSKGGAGGDGATHGEGASHVGER
                     AAPHPVRASPPEEFTGRFETGPRKGAAGGLSAVVESVKHAWREMGAARSLKTLLKVNQ
                     KDGFDCPGCAWPDPEGERSHFEYCENGAKAVAEEATVRRVTADFFREWSVADLSCKSD
                     YWLGKRGRITGPVILRRGATHYEPLSWDEAFATIAAELNALGSPDEAAFYTSGRTSNE
                     AAFLYQLFVRQFGTNNLPDCSNMCHESSGTALTATIGVGKGTVTLADFDLARCIFVIG
                     QNPGTNHPRMLTALQRAKKRGCKIVHVNPLPETGLQRFKHPQHPLDMLGKGTRLSDLF
                     LQVRVNGDVALLKGIMKEVLEEEERRPGQVLDARFIAEKTAGFAEFAAALKRTGWDDI
                     VEQSGVARESLREAAHLFIESERTIICWAMGLTQHKNAVANIQEIVNLLLMRGQIGKP
                     GAGACPVRGHSNVQGDRTMGVWERPTEVFLDELARVFRFEPPRRHGLDTVGTIQAMHA
                     AAVKVFFALGGNFLSATPDTEYTAEALRNCALTVQVSTKLNRAHLVTGRTAVILPCLG
                     RSERDVQP"
BASE COUNT         1436 a         2951 c         3243 g         1516 t
ORIGIN      
        1 tgctcgacgg gagcgcggag aaggcgtccg cgcttttgac ggacgcgcac gcgtcggggc
       61 gcggactcgc cgcgctgttc gacgaggtgc tgtgccccgc gatgcggagg gtcggagacc
      121 tctggcacga gggcgagttg acggtcgcgc aggagcacgt cgccacgcgc gccgcgctct
      181 cggccctgca aaccctcggc ggcgcactcg cggaggcgga gccgttcgcg ccgctcgccg
      241 tctgctgcgg cgcggaagat gacttccacg agctgccggt caactgctcc gaactcgtct
      301 tgcacgaggg cggctgggcc gtcctgaacc tcggcgcgaa cacgcctttc tacgcgctcg
      361 ccgaagcggt cgcgcggcgc gggccgcggc tcgtcgcggt ggcggcgacc gtcctcgcag
      421 acctcgaccg cgccgtcagc gagttcaagg acttgcgcgc cgccgccgcg cgcgcgggcg
      481 cggcggtcgt gctcggcggc gcgggcttcg ccgacgcggc ggtgcgccgc cggctccccg
      541 cagacctcca cgccgacagc ttcatgcaac tgtcggaact ctccgagaag aagaagaatg
      601 aagtgatgag gactgaggac tgaaaaccga gtaaagacgg gcgacgagtg acgagtgata
      661 agagggatgt agttcggact cgtcacggct gacggttcac ggttcaccgc tcacggctct
      721 tactcagtcc tcagtcctca tcacttcttc cccggcaggt ggccgaggtc tgtgaggatg
      781 ctcttggtga tgcggaggcg ctcgtcgcgg atggaggcga aggtggggcc ttcgatctgc
      841 gtggcgaaga cgtaggcgtt gtctttggtt tcgaggtagc cgacgaacca gccgaggaag
      901 cggccctcgg cgaaggggcc gccgccggtc ttgccgctca gcttgtagcc cgcgccctct
      961 tcgttcacga agatgttttt gaagacggcg tacgtgcgct tcgagacggg cagctcttcc
     1021 ctgtagagcg ccttcaggaa ctcgacctgc tcgtcggcgg aaatcttcag cgagctgccc
     1081 agccagaagc gatccacgcc gcccgagatg tcctgggtgc cgtagcggaa cttgttgacg
     1141 aactcgcgca tcttctctgc gccgacgcgc gaggccagct cgcggtagta ccagacgacc
     1201 gagttgcgga aggcggagcg cagcgtgtga tcccgccacc aaaagtcgaa ctgtccctcg
     1261 cgcgggtact tctcggcgtc ccacttgatg aggaagtcgg cgtccttgat gacgccggtt
     1321 tccagcccga tgagcgagtt cggaatcttg aaggtcgaga agggcgagaa gcgcgcggcg
     1381 gcgcgcgccg ggtcgtggcg cacgtagcgg tcgcgcttca agtcgtagag cacgaacgtg
     1441 ccggggaact gttcgaagtg ccgggcgagg tcgggctgcg tcgcgtcttt gtcaaccacc
     1501 gtcgagggcg tggccgtggt cgaaggcgtg acggcccgcg gcgacggcgg cgtctgaggg
     1561 gcggcgacgt ggctcgtctc gtcggccctc gcacaagcct gcgccgagag cagggcggcg
     1621 gacaggatgg cggcggtcaa gatggtcttc gtgtgtctca tcctgccgct aagataacaa
     1681 ttcgcttgac gagaatacag ttcgggtcgg gcgatgtggt ctacatcatt cttgcatcgg
     1741 cggcggcttg aacttcaacg cagagggcgc ggaggaggcg cagagggcgc ggagaaaatc
     1801 aaagacggat tcgaagcttc gaatcccgaa attcttctct gcgctctctg cgtgttctct
     1861 gtgaactctg tgtcgaaagc cgacgcgcgg cgtcactcag gatgaatgaa acccgaggcg
     1921 tgcggaagtc tcgggcgagc ggtcagaccg ctgccgtcgt cgccgtcgtc gccttcttca
     1981 agccgacgta ttcgagcgcg gcgaacatcg cgacgccgag cgcctgtgcg atgacgaagg
     2041 cgtagccggg cggcgtgggc gcgacccagc cggagagcag caggagcagg ctgtccaacg
     2101 tccagagcac gttgagcacg atgacggccc agaccgccgc cgtcgggagc gtctcgcgcg
     2161 tggcgaggta gacgaggaac gcggcgaagg gcagcaggcc gacgccggcg tagcgcgaca
     2221 gccccgacgg caggccgaag aactgttcga gcagcccggc gccgagcgtc atcagcagcc
     2281 ccgcggcgag gcacgtcgcc gcatcggcga gcagcacgcg gcgcaagaat gtcgaagtgg
     2341 gaaatgactg catggcgaat cctccaaacc tctttcgagg ctttcgttga cttcggctcg
     2401 ggcgacgggc ttctctctaa ggtcacgcga ccgtcccgtc cgccatgcgc gcatcatggc
     2461 agccttcgcc atgcgaaaac aattacttcg gaggtaatgg ttggacgcgc cgaagttgtg
     2521 ataacttcgg ggccatgatg ggcgaagtca gcacccggcc cgtgggcacc ctgctgcgcg
     2581 actggcgccg ccgccgccgc ctcagccagc tcgacctcgc gctcgaagcg gacatctctt
     2641 cgcgccacct gagctttctc gaaaccggtc gctcgcagcc gagccgcgag atggtcctgc
     2701 gcgtcgccgc gcggctcgaa gtccccttgc gcgggcgcaa cgcactgctc gtcgccgcgg
     2761 gctacgcgcc ggtcttcaaa gagaggccgc tcgaagaccc ggcgctcggc gccgcgcgca
     2821 gggcggtcga tctcgtgctc gcggggcacg agccgttccc cgcgctcgcc atcgaccgcc
     2881 actggacgct cgtcgccgcg aaccgcgccg tcccgctcat gctcgcgggc atcgaccccg
     2941 ctgtactcgc gccgcccgtc aacgtgctgc gcctcagcat gcacccgcgc ggactcgcgc
     3001 cgcgcatcgt caacctcgcc gagtggcgca cgcacctgct cgcgcgtctg cggcggcaga
     3061 tcgaagcgac cgccgacgcc gtgctcgccg agctgttcga agagctgagc cgctaccccg
     3121 tgccggacga cggcgggcac gacgcgggcg ccgcgaccga ctacgcgggc gtcgccatcc
     3181 cgctccaact tgcgaccgaa ggcggcgtcc tctcgttcct cagcatcacg accgtcttcg
     3241 gcacgcccgt cgatatcacc ctctccgaac tcgcgctcga atccttcttc cccgccgacg
     3301 ccgccacctc cgacgcgctc aatcgaatca tgaagcggga ctgacggcgg ggttcccgtc
     3361 gaactccttc acggactttc gcgcgggccg agcagttcgt cgaaggcgtt gtcgggcagc
     3421 tcttcgagcg tgcggacgac gatgtccgcg ccgaggtcgg cgtgggcggt gaggacgccg
     3481 acggcgcgca tgcccgcgcg gcgggcggcc tcgacgccgg agggcgcgtc ttcgacgacg
     3541 acgcaggaag agggcgcgac ttcgagccgt gcggcggcgg tgaggaagac ctgcgggtcg
     3601 ggcttgccgc gcgtcacgtc ttcggcggag acgattgatg cgaagtagtc tccgatgccg
     3661 agcgcgccgg tgatcgcttc gaggttggcg cgcggcgcgg acgaggccat cgcctgccgc
     3721 cagccttcgc ccctcaactt gtcgagccag cggcggacgc cgggcaggag cgtgatgccg
     3781 cgctcgcgga cgagcgcgcg gtagagcgtc tccttcgcgt cgccgacgcg cgcgacctcc
     3841 gcggcgggga atcccgggcc gaaatactcg cgcaggattt cgtcgttgcg ccgaccgaag
     3901 atggaggcga actgctcgcg cgtgagcggg aagttttcgg cggcgagcgc gtcgcgccag
     3961 gagagccagt ggtactcgct cgaatcgatg agggtgccgt ccacgtccca gaggacggcg
     4021 cgcgtcggtc gcagggtcga aggttcgtgc gcggtcatgg gcgacgaatg taaagcatgg
     4081 ggcggcggtt gccaaacgcg cgggccgggt cgttcggtcg gcgtgcgtgc ggacaaaggg
     4141 ggcggcgcgc ggtatgatga gcggcacgga gcgaactcga aatctttgtg aaggcgggag
     4201 gtgaagtgtg aacacagtcc tgctcggcgc gctcgcgggt ttcgcggcca ccgcgccgat
     4261 gtcgctggcg atgaagctga tgcacgagca actgcccacg cacgagcagt accctttgcc
     4321 gccgcgcgag gtggcgatgg aggtcgccga aaaagcgggc atcaagcacg aactcgacga
     4381 gccggagcgc gaggggctga cgtgggcctc gcacttcgcc tacggcgcgg cgagcggcgc
     4441 ggtctacgcc ccgctcgcgc gcgcggtcga tctgccgccg ccgctgacgg gcgcggcgtt
     4501 cggcctcgcc gtgtgggccg ggagttatct cggctggctc cccgcggctg gcgtcttgcg
     4561 gcccgcgacc gagcacccgc cgcgccgcac ggcgttgatg atcgccgcgc acgtcgtgtg
     4621 gggcgtcgcg gcgggcgtga ttattgacca actgtccgac gggcggcgcg ggccgcaggg
     4681 cgggcggcga agcaggtgag cgacgcggtc gccggatgtt tgacgagcgg cgcggtcgcc
     4741 ggggatgtgg cgggcggcgc gatgaagacg gaggacggag cggcgaaggc cgagccgtcg
     4801 cccgtcgagg cgccgcccgc gggtcgctgg cgcgcgctcg tgctgctggc ctgcgcggag
     4861 ctgctgtgca tgtcgctgtg gttctccgcg tcggcggtcg tgcccgcgct gcaagcggag
     4921 tggcgactga cggaggcggg cgcgagctgg ctgacgctcg ccgtgcagct cggcttcgtc
     4981 gcgggcacgc tcctctccgc gctgctcaac ctcccggaca tcatcagcac gcgccgcctg
     5041 gtggtcgccg ccgccgtctg cgccgcggtc gtcaacgccg cgttcggact gttcgcacat
     5101 gatttgacga cgggcgtggt gctgcgcttc ctcgtcggtc ttttcctcgc gggggtgtac
     5161 ccgccgggga tgaagatcat ggcgacgtgg ttccggcggg ggcgcggggc ggcgctgggc
     5221 gtgctggtgg gcgcgctgac gctgggcaag gcggcgccgt atctggtcaa cgcggtgggg
     5281 agcgacgagt ggcgcgtgaa cgtgctgttc gcgtcgctgc tggcggcggt gggcgcgttc
     5341 gtcgtgctgc tgttcgtggg cgacgggccg tacgggacgc cgcccgcgcg gttcgacata
     5401 aggcaggcgg cgcgcgtctt cgggaaccgc ggcgtgcggc tcgccaactt cggctacttc
     5461 ggccacatgt gggagctgta cgcgatgtgg acgtggctgc ccgtgatgat gcgcgcggcg
     5521 ctggccgaga ggggcgagcc ggcggcgctg gccgaggtgg cttcgttcct tgtcatcggc
     5581 gcgggcgcgt tgggctgcgt ggcggcgggg ctggtggccg accgcgcggg ccggacgctc
     5641 gtcacgtcgg cggcgatggt ggtgagcggc gcgtgctgtc tgctcgtcgg gttcacgttc
     5701 gaggcgggga gcacggcgct gctgctggcg gtcgcggtgg tgtggggcgc gagcgtcgtg
     5761 gcggactcgg cgcagttctc ggcgtgcgtg acggaactcg gcgacccgcg ctacgtgggc
     5821 acggcgctca cgatacagac gtgctgcggg ttcctgctga cgaccgtctc gatccacctc
     5881 gtcccgctgc tcgtccgaca catcggctgg cgctacgcct tcgccgcgct cgcgcccggc
     5941 cccgtcttcg gcgtcgtctc gatgctgcgc ctgcgcgcgc tgcccgaagc gtcacgaatc
     6001 gcgggcggca aacgttagaa gggtgttggg tgttggtgaa aagcagatgt cttccccaac
     6061 accccgtctt taatcaagga gggttcgcat gaaaaagcac gggccgtgga cggtcaagtc
     6121 ttcggagcgt ttgtatcagg ataagtacgt cgagttccgt gtggacgagg tggtgaagcc
     6181 gagcggcgag ccgagccgtt acgcgaccgg gcggatgctg ccgggcgtgt cggtgctggc
     6241 gctggacgcg gaggggttcg tccacctgac gaagcagttc cgctacgcgg tcggcaggga
     6301 gtcgatagag gtggtgagcg gcgggctgga cgagggcgag gaggaggggc gggcggccct
     6361 gcgcgagctg cgcgaagaac tcggcatcga ggccggcgag ctggtcgatc tcgggctggt
     6421 ggacgccgtc acgtcgcagg tctcggcgcc cgcgcggatt tacctcgcgc gcgagctgaa
     6481 gttcggcgaa cccgaccgcg gcggctcgga gttgatggag acgctcaggg tgaagctcga
     6541 agaggccgtg cggatggtga tggcgggcga catcacgcac gcgataagct gcgtgctgat
     6601 actcaagacc aaagaagtac tgaggactga gtgaaagcaa agaaggactg aaggactgag
     6661 tagaagcgtt gccttcatcc tcagtcctca gtcctcagtc ctcaatcctt cttttacgct
     6721 ttggccgttt tggtctggcc ttcctgacgc ttcttctcct cttccatccg ggcgccgagt
     6781 tgttcgatct gctcttcgga gagcacgtcg cgcgccttct ggaacatctc gccctcttcc
     6841 tcttcgacgt ggtgctcgac gttctcctgc aagaccttga gcttggccgc ccactgctcc
     6901 gtgtccacgg gcatcccttc gagttcctgc aagagcatct tgatgacgtg gtgctcctcg
     6961 aagccttcga gcacgatctc gcgcgtctcc gcctcgcgct tgatggaggg gtagaagatc
     7021 gtctcctcga tgtgcgcgtg gatgtcgaga ttcgtcttca gctcggcgaa caactcgctg
     7081 cgcgtcttga cggcgcgctc ggtggtcggt tcgagctgct ggaaaatcgc cgacaccttc
     7141 ttgtggtctt ccttcagcag ttgaaaagcg ttcatatcaa tccccctgtt gtattcctct
     7201 ttgcacacgc ttgcacggta acgatacgcg ccaagcgccg tttctataaa ctcactcaag
     7261 cacagtgcca ctgggacaaa tgatgaatgc ggaatgcgga atgatgaata aaaacaacgt
     7321 cttcctattc atcattcatc attccgcatt catcagttct gcacgcgctc cccggcggtc
     7381 ggacttctgc cgttgaagtg tgatgaagta aacttgagcg cgtcatgacg aacgaggaga
     7441 aagtgagcga ggagacggcg tccgccgcgc agccgtcgca ttacaacgtg agcaagggcg
     7501 gggcgggcgg cgacggcgcg acgcacggcg agggcgcatc gcacgtcggc gagcgggccg
     7561 cgccgcaccc cgtgcgcgcg tcgccgcccg aagagttcac ggggcgcttc gagacggggc
     7621 cgcgcaaagg tgccgcgggc ggtctctcgg cggtcgtcga gagcgtgaag cacgcgtggc
     7681 gcgagatggg cgcggcgcgc tccttgaaga cgctgctgaa ggtgaaccag aaggacggct
     7741 tcgactgtcc gggctgcgcg tggcccgacc ccgaaggcga gcgctcgcac ttcgaatact
     7801 gcgagaacgg cgcgaaggcc gtcgccgaag aagcgaccgt gaggcgcgtc accgcggact
     7861 tcttccgcga gtggagcgtc gccgatctct cgtgcaaatc cgactactgg ctcggcaaac
     7921 ggggccgcat caccgggcct gtcatcctcc gccgcggcgc gacgcactac gaaccactga
     7981 gctgggacga agccttcgca acaatcgccg ccgagctgaa cgcgctcggc tcgcccgacg
     8041 aggccgcctt ctacacttcg ggccgcacgt cgaacgaggc cgcgttcctc tatcaacttt
     8101 tcgtccgcca gttcggcacc aacaacctgc ccgactgctc gaacatgtgc cacgagtcct
     8161 cgggcacggc gctcacggcg accatcggcg tcggcaaggg caccgtcacg ctcgcagact
     8221 tcgacctcgc ccggtgcatc ttcgtcatcg ggcagaaccc cggcaccaac cacccgcgca
     8281 tgctcaccgc cttgcagcgc gcgaagaagc gcggctgcaa gatcgtccac gtcaacccgc
     8341 tccccgagac cggcctccaa cgcttcaagc acccgcagca cccgctcgac atgctgggca
     8401 aaggcacgcg gctctccgac ctcttcttgc aggtgcgcgt caacggcgac gtggcgctgc
     8461 tgaagggcat catgaaggag gtgcttgagg aggaagagcg gcggcccggc caggtgctcg
     8521 acgcgcgatt catcgcggag aagaccgcgg gcttcgcgga gttcgccgcc gcgctgaagc
     8581 ggacgggctg ggacgacatc gtcgagcagt cgggcgtcgc gcgcgaaagc ttgcgcgagg
     8641 cggcgcacct cttcatcgag tccgagcgca ccatcatctg ctgggcgatg ggcctgacgc
     8701 agcacaagaa cgcggtcgcc aacatacagg agatcgtcaa cctgcttctc atgcgcgggc
     8761 agatcggcaa gccgggcgcg ggcgcatgcc ccgtgcgcgg ccactcgaac gtgcagggcg
     8821 accgcacgat gggcgtctgg gagcgaccga ccgaagtgtt cctcgacgag ctggcgcgcg
     8881 tcttccgctt cgagccgccg cgccgccacg gcctcgacac cgtcggcacg attcaagcca
     8941 tgcacgccgc cgcggtcaaa gtcttcttcg cgctcggcgg caacttcctc tcggccacgc
     9001 ccgacacgga gtacacggcg gaagcgctgc ggaattgcgc actcaccgtg caggtttcga
     9061 cgaagctcaa ccgggcccac ttggtgaccg gccgcacggc cgtgatccta ccttgtcttg
     9121 gccggagcga gcgcgatgtg cagccg
//
LOCUS       DBNGOH010000764         1506 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4146045, whole genome shotgun sequence.
ACCESSION   DBNGOH010000764 DBNGOH010000000
VERSION     DBNGOH010000764.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1506)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1506)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1506
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4146045"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..280)
                     /locus_tag="AC159P_14745"
     CDS             complement(<1..280)
                     /locus_tag="AC159P_14745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016329157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HIR3696757.1"
                     /translation="MSTAAPISETTNNIPRINDPAPEFEAKSTQGQLKLSDYTGKWVV
                     LFSHPADFTPVCTTEFVEFARRSEEFKERGAQLVGLSVDSVPAHIAWIR"
     gene            737..1456
                     /locus_tag="AC159P_14750"
     CDS             737..1456
                     /locus_tag="AC159P_14750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/TrbI/VirB10 family type IV secretion system
                     protein"
                     /protein_id="HIR3696758.1"
                     /translation="MTIKSNNTARRAASVLVMFALILVTVIPASAQRRRARTRRAPAR
                     VTRPAVRFFTVPADTVIRVRMDSQMSSRSARVGDRFSTTVTEPVYGGDSGAEVIPVGS
                     KIWGRVTSVKQAGRRSPGNISVSFYQIATPSRATYPINGSLSSLQADDVNADNEGTVA
                     GKGNRKRDAVFIGGGAATGALIGAIAGGGKGAAIGAILGGAAGTGARVYEREEEAEVK
                     SGTEFGVILNRGVSLPEYRAQ"
BASE COUNT          264 a          491 c          511 g          240 t
ORIGIN      
        1 tcctgatcca ggcgatgtgc gccgggacgg agtccacgga gaggccgacg agctgcgcgc
       61 cgcgctcctt gaactcttcg gagcggcgcg cgaactcgac gaactcggtc gtgcagacgg
      121 gcgtgaagtc cgccgggtgc gagaagagta ccacccattt gcccgtgtag tcggacagct
      181 tcagttgccc ctgcgtggac ttggcctcga actcgggggc ggggtcgttg atgcggggga
      241 tgttgttcgt ggtctcggat atcggtgccg ctgtgctcat gttctgctca ctcctctgcg
      301 cgatgtgcgt tgtcaaccga attgataatt ccgcctcaag ggaagaaggc gcgagcctgc
      361 ccgcgcgtgc gcacgccgac cgaaatatcc cgtgagtctt tggaggcgca agcttacaac
      421 atttcaccgg tttttcaaca cccgccgacg cctccgccgc cggagagcac ggacgggaga
      481 gaagcactcc ctccgtcttc ggacttatac gatatagtgc gcgggcggcc cctcatcacg
      541 cacttcgtcg agacgcgggc gccccgccgc cgctggcgtt tagctcgaaa atccgataaa
      601 tacacgtcaa ccggcgtttt ccaaaacact gctttccgtg gcacgcacat tgccaaatta
      661 cgggcggcgg tcgctgatgt gcgacccggc gaaggacgcg gggcgaccga gccgactgag
      721 gagggactga aaagtgatga cgataaaatc aaacaacacc gcgcgacgtg ccgcgagtgt
      781 gctggtcatg ttcgcactca tcctcgtgac cgtgatcccg gcctccgcgc agcgccgcag
      841 ggcgcgcacg cgccgcgccc ccgcgcgcgt gacgaggccc gccgtccgtt tcttcacggt
      901 gccggcggac acggtcatcc gcgtccgcat ggactcgcag atgagttcgc gctcggcgcg
      961 cgtcggcgac cgcttctcga cgaccgtgac cgagccggtc tacggcggcg acagcggcgc
     1021 cgaagtgatt ccggtcggca gcaaaatctg gggccgcgtg acctcggtca aacaggctgg
     1081 gcgccgcagt ccgggcaaca tctccgtgag cttttatcag atcgccacgc cgagccgcgc
     1141 cacgtacccg attaacggct cgctgtcgag ccttcaggcc gacgacgtga acgccgacaa
     1201 cgagggcacg gtcgcgggca agggcaaccg caagcgcgac gcggtcttca tcggcggcgg
     1261 cgcggcgacc ggtgcgctca tcggcgccat cgcgggcggc ggcaagggcg cggccatcgg
     1321 cgccatcctc ggcggggcgg cgggcacggg cgcgcgcgtc tacgagagag aagaggaggc
     1381 cgaggtgaag tcgggcaccg agttcggcgt catcctcaac cgcggggtct cgctgcccga
     1441 gtaccgggcg cagtaagact cagacgagat cggcggcccc acggggcggc cgtatcggag
     1501 gaagcc
//
LOCUS       DBNGOH010000765         4658 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3964913, whole genome shotgun sequence.
ACCESSION   DBNGOH010000765 DBNGOH010000000
VERSION     DBNGOH010000765.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4658)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4658)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4658
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3964913"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2272
                     /locus_tag="AC159P_14755"
     CDS             <1..2272
                     /locus_tag="AC159P_14755"
                     /inference="COORDINATES: protein motif:HMM:NF051698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HIR3696759.1"
                     /translation="TYDFDFIFAAGSIDFNTTNAAVGFTAANLQLLSRTGAGDAPTID
                     TNDQTTARGLVNNLVGAIGAVDMRFNVSSLDSGFVQGEPTRRTYRNREFDLFFQDTWA
                     LKKNLTLNLGLRWEYSTVPVETNGLLLIPDGGFDAVFGVSGREGFFNPGVLNGTPCSA
                     LQGLPRARNLSNAVALIETCSTRYVPGGANNGRPLWDDDLDNFGPVVSFAYDPFKDGK
                     TSIRAGFRLSYFQDVFGIIEGNVDDNEGLQVTQSCIPTDGTCTNNPTLLRNLAATGAP
                     LPRLPVFRLPAFRSILDTAAQDFRTYQPDLGTSYYREWTVGIQRELFKNASVEARYVG
                     NRGRALRRIADFNEINIFARDAVTGQSFLDAFLIAQQNLACNNASTVAANQGRFDTLN
                     LPCSRPNPLMDALIAGEPARLRNRPTLLEALAFNAPGEFAYRLMHSETSTAGTGLPAA
                     DRIRGGSFWGAVLAGRLPVNFFQVNPFIASSRALVNDGRSAYDALQVEFQQRLSKGVT
                     FQVNYTFGKSLSDYDGDANEYLNDTRPSSVRNLDYTRQLTMPRHQVNANWVAELPFGR
                     GKRFLSGTKGLWRRLVSGWQTGGIVGFRTGRPLTITSGIGTFHRSAISGDNTVNLSQP
                     LTAPELRALTGRRDIAGGIFWLDPCTSSFTGAACQSADAIQGLFRLPNPGELGQLPQT
                     VIYGPTRFTADFNLSKRTRLGEGHHIEFRWEVFNVFNNVNFNVPVTDIFSPSFGQITR
                     TVTNPRLMQFALKYNF"
     gene            2315..4177
                     /locus_tag="AC159P_14760"
     CDS             2315..4177
                     /locus_tag="AC159P_14760"
                     /inference="COORDINATES: protein motif:HMM:NF013206.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HIR3696760.1"
                     /translation="MRLETRLFRQLAASVLAAAALMQAPLPASSADTFRPVVRGRRGV
                     VAGGHPLAVEAGLRLLQRGGNAVDAGVATVLAASVIEFSHFSFGGEVPMLIKLRGKEV
                     AVVEGMGAAPLKATREFFVSRAGGANPAGGAATMPGAKSGAIPSTGPLSATVPAVLDA
                     CVVALDQFGTKTLGEVLQPAIELADGHPIDELRVQYIRTRASIFAQWPDAKRVFMPGG
                     QVPKVGDIFAQPDLARTLREIVDAEKRASAGGRRAALAAARDHFYKGPIGKRIGDYMQ
                     QNGGLLAAEDLARFRAKTGAPARGQYRGYEVYKAGFWTQGPVMVEALNVLSGFDLQKM
                     GHNSADYVHTLTEALKLGFADRDRYYGDPDFVKVPERELLSEEYAALRRSLINRERAS
                     QEQQPGDPARMKPLAETSRAKLDTGESTVHPLERANDTTCVNVIDKDGNLFSATPSGA
                     WLPAVVAGDTGVLMTQRLQSALLEEGHPNVVAPGKRPRITLSPTLVTRGGEPFMVLST
                     PGGDNQDQALLQVMLNVVEFGMNPQEAVEAPRFDTQHLVSSFDDHRFEAGSLNVESRL
                     GEEITGQLKSRGHRVRVQGAWGTLSAPTVIVYDPRTGVAAAGADPRRGRYAVAW"
     gene            4280..4585
                     /locus_tag="AC159P_14765"
     CDS             4280..4585
                     /locus_tag="AC159P_14765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696761.1"
                     /translation="MNDSDEKFALEYYTADDPDPQGPPFKYRSHYLDDAVAEAWRLRD
                     AGNRPLRVTRGDAVTLDEGALESTLARVSELGKGELEQPLREIAARALNEDAKAHGA"
BASE COUNT          780 a         1790 c         1407 g          681 t
ORIGIN      
        1 cacctacgac ttcgacttca tcttcgccgc cggctccatc gacttcaaca ccaccaacgc
       61 cgcggtcggc ttcacggcgg ccaacctgca gctcctctcg cgcacgggcg cgggcgacgc
      121 gcccaccatc gacaccaacg accagaccac cgcgcgcggc ctcgtcaaca acctcgtcgg
      181 cgccatcggc gccgtggaca tgcgcttcaa cgtctcttcg ctcgactccg gcttcgtcca
      241 gggcgagccg acgcgccgca cctaccgcaa ccgcgagttc gacctcttct tccaggacac
      301 gtgggcgctc aaaaagaacc tcaccctgaa cctcggcctg cgctgggaat actcgaccgt
      361 gcccgtcgag accaacggcc tgctgctcat ccccgacggc ggcttcgatg cggtcttcgg
      421 cgtctcgggc cgcgaaggct tcttcaaccc cggcgtgctg aacggcaccc cctgctccgc
      481 gctgcaaggg ctaccccgcg cgcgcaacct gtcgaacgcc gtcgcgctca tcgaaacctg
      541 ctccacgcgc tacgtgcccg gcggcgcgaa caacgggcgc cccctctggg acgacgacct
      601 cgacaacttc ggccccgtcg tgagcttcgc ctacgacccc ttcaaggacg gcaagacctc
      661 gattcgcgcc ggcttccgcc tcagctactt ccaggacgtg ttcggcatca tcgagggcaa
      721 cgtggacgac aacgaaggct tgcaggtcac gcagagctgc atcccgaccg acggcacctg
      781 cacgaacaac ccgacgctgc tgcgcaacct cgccgcgacg ggcgccccgc tgccgcgcct
      841 gcccgtgttc cggctccccg ccttccgcag catcctcgac accgccgcgc aggacttccg
      901 cacctaccag ccggacctcg gcacttctta ctacagggag tggaccgtcg gcatccagcg
      961 cgagctgttc aagaacgcct cggtcgaggc gcgctacgtc ggcaaccgcg gacgggcctt
     1021 gcggcgcatc gccgacttca acgagatcaa catcttcgcg cgcgacgcgg tgacgggcca
     1081 gtccttcctc gacgcgttcc tcatcgcgca gcagaacctc gcctgcaaca acgcgagcac
     1141 ggtcgccgcc aaccaggggc gcttcgacac gctcaacctc ccgtgcagcc gcccgaaccc
     1201 cctgatggac gccctcatcg cgggcgagcc ggcgcgcctg cgcaaccgcc cgacgctcct
     1261 cgaagcgctc gccttcaacg cgcccggcga gttcgcctac cgcctcatgc acagcgagac
     1321 ctcgaccgcc ggcacgggcc tgcccgccgc cgaccgcatc cgcggcggca gcttctgggg
     1381 cgcggtgctc gcgggccgcc tccccgtcaa cttcttccag gtgaacccgt tcatcgccag
     1441 ctcgcgcgcg ctcgtcaacg acgggcgctc ggcctacgac gcgctccagg tcgagttcca
     1501 gcagcgcctc tcgaagggcg tcaccttcca ggtcaactac accttcggca agtcgctctc
     1561 ggactacgac ggcgacgcca acgaatacct caacgatacg cggcccagct cggtgcgcaa
     1621 cctcgattac acgcgccaac tgaccatgcc gcgccaccag gtcaacgcca actgggtcgc
     1681 cgagcttccg ttcgggcgcg gcaagcgttt cctctcgggg acgaagggct tatggcgccg
     1741 cctcgtcagc gggtggcaga ccggcggcat cgtcggcttc cgcacgggcc gcccgctcac
     1801 gatcacctct ggcatcggca ccttccaccg ctccgccatc tccggcgaca acaccgtcaa
     1861 cctctcgcag ccgctcaccg ccccggagct gcgcgcgctc acaggccgcc gcgacatcgc
     1921 gggcggcatc ttctggctcg acccctgcac gtcgagcttc accggcgccg cctgccagag
     1981 cgcggacgcc atccagggac tcttccggct gcccaacccc ggcgaactcg gccagctccc
     2041 ccagaccgtc atctacgggc cgacgcgctt caccgccgac ttcaacctct ccaagcgcac
     2101 gcgcctcggc gagggccacc acatcgaatt ccgctgggag gtcttcaacg tcttcaacaa
     2161 cgtcaacttc aacgtccccg tcacggacat cttcagcccc agcttcggcc agatcacgcg
     2221 caccgtcacc aacccgcgcc tgatgcagtt cgcgctgaag tacaacttct gaaccgggac
     2281 cgccgacata cgccaccgag gggtgctcca cgttatgcgt cttgaaactc gactcttcag
     2341 acagctcgcg gcctccgtcc tcgccgccgc cgccttgatg caggcgccgc ttccggcgtc
     2401 ttccgccgac acgttccgcc cggtcgtgcg cgggcggcgc ggcgtggtgg cgggcgggca
     2461 cccgctcgcg gtcgaggccg ggctgcgcct cttgcagcgc ggcggcaacg ccgtggacgc
     2521 gggcgtggcg accgtgctcg cggcctccgt catcgagttc tcgcacttct ccttcggcgg
     2581 cgaggtgccg atgctgatca agctgcgcgg caaggaggtc gcggtcgtcg aaggcatggg
     2641 cgcggcgcct ctgaaggcca cgcgcgagtt cttcgtgagc cgcgcggggg gcgcgaaccc
     2701 cgcgggcggc gcggcgacca tgccgggcgc gaagtccggc gccatccctt cgacggggcc
     2761 gctctctgcg accgtgcccg ccgtcttgga cgcctgcgtc gtcgcgctcg accagttcgg
     2821 caccaagacg ctcggcgaag tcttgcagcc cgccatcgaa ctcgccgacg gccaccccat
     2881 cgacgagctg cgcgtccagt acatccgcac gcgcgcctcc atcttcgcgc agtggcccga
     2941 cgccaagcgc gtcttcatgc ccggcgggca ggtgcccaaa gtcggcgaca tcttcgcgca
     3001 gcccgacctc gcgcgcacct tgcgcgagat agttgacgcc gagaagcgcg cctcggccgg
     3061 cggtcgccgc gcggcgctcg ccgccgcccg cgaccatttt tataaaggcc ccatcggcaa
     3121 acgcatcggc gactacatgc agcagaacgg cggcctgctc gcggcggaag acctcgcgcg
     3181 cttccgtgcg aagaccggcg ctccagcgcg cggccagtat cgcggctacg aggtctacaa
     3241 ggccggcttc tggacgcagg ggccggtcat ggtcgaagcg ctgaacgtcc tctccggctt
     3301 cgacctgcaa aagatggggc acaactccgc cgactacgtc cacaccttga cggaggcgct
     3361 caagctcggc ttcgccgacc gcgaccgcta ctacggcgac ccggacttcg tcaaagtccc
     3421 cgagcgcgaa ctgctctccg aagagtacgc ggccctgcgc cgctctctga tcaaccgcga
     3481 gcgcgcctcg caggagcaac agcccggcga ccccgcgcgc atgaagccgc tcgccgaaac
     3541 ttcgcgcgca aaactcgaca ccggcgagtc aacagttcat ccgctcgaac gcgcgaacga
     3601 tacgacgtgc gtcaacgtca tcgacaaaga cggcaacctc ttcagcgcga cgccctcggg
     3661 cgcgtggctc ccggcggtgg tcgcgggcga cacgggcgtg ctgatgacgc agcgcctcca
     3721 atccgcgctg ctcgaagagg gccaccccaa cgtcgtcgcg cccggcaagc gaccgcgcat
     3781 cacgctctcg cccacgctcg tcacgcgcgg cggcgagccg ttcatggttt tgagcacgcc
     3841 cggcggcgac aaccaagacc aagccttgct gcaagtgatg ctcaacgtcg tcgagttcgg
     3901 catgaacccg caggaggcgg tcgaggcgcc gcgcttcgac acgcagcacc tcgtcagttc
     3961 cttcgacgac caccgcttcg aggccggctc gctcaacgtc gaatcgcgcc tcggcgaaga
     4021 gatcaccggc cagctcaagt cgcgcggcca ccgcgtccgc gtgcagggcg cgtggggcac
     4081 gctctcggcc cccaccgtca tcgtctacga cccgcgcacg ggcgtcgccg ccgccggggc
     4141 cgacccgcgc cgcggtcgct acgccgtcgc gtggtgaaga ggagtctgga gtctgaagtc
     4201 tggagtctga agtcagaacc aacggcagtc ggctcggact ccagactcca gactgttttt
     4261 aagcgaaggg taggatgata tgaacgacag cgacgagaag ttcgcgctcg aatactacac
     4321 ggcggacgac ccggatccgc aggggccgcc cttcaagtac cgctcgcact acctcgacga
     4381 cgccgtcgca gaggcgtggc ggctccgcga cgcgggcaac cgccccctgc gcgtcacgcg
     4441 cggcgacgcc gtcacgctcg acgagggagc gctcgaaagc accctcgcgc gcgtctccga
     4501 actcggcaaa ggtgagctgg aacaacccct gcgagaaatc gccgcccgcg cgctcaacga
     4561 agacgcaaag gcgcacgggg catgacgagt gagcggtaag cggtgggcag ttgaaatcaa
     4621 gatggtctgc ggttgagtgt ggcgcgcgat tggtcgta
//
LOCUS       DBNGOH010000766         5707 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2314180, whole genome shotgun sequence.
ACCESSION   DBNGOH010000766 DBNGOH010000000
VERSION     DBNGOH010000766.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5707)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5707)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5707
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2314180"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..383
                     /locus_tag="AC159P_14770"
     CDS             <1..383
                     /locus_tag="AC159P_14770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015152245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696762.1"
                     /translation="AKVEKNWLEWIVFGVGLVLVAGVLGFLVYDGARMGQAPPDIELK
                     LGAPERRGAGFVVPVAATNRGDETAEGVQIEVVLEAGGGEPERGEFAIAFLPRRATRH
                     GWVTFRTDPGTGQLKARVLGYEKP"
     gene            471..686
                     /locus_tag="AC159P_14775"
     CDS             471..686
                     /locus_tag="AC159P_14775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696763.1"
                     /translation="MDIKSWSQAKAELAVDALVDAGLVRAEDFERAVAIVAEEILVRL
                     LLGDYPPPEESQTPDPTARQRIQPERE"
     gene            803..1219
                     /locus_tag="AC159P_14780"
     CDS             803..1219
                     /locus_tag="AC159P_14780"
                     /inference="COORDINATES: protein motif:HMM:NF039957.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6010 family protein"
                     /protein_id="HIR3696764.1"
                     /translation="MIALLLGAVLSLALVGAARMFAPGRERLVYAVGLVLTALVYVVF
                     AVAGGASARWAAIESLGVLLYGAAAWGGLRGLLRLLAAGWAAHVAWDVLLHLSGAGAE
                     YTPHWYPWLCVSFDLIMAGAVLASSRRGAADLRGIA"
     gene            1442..2326
                     /locus_tag="AC159P_14785"
     CDS             1442..2326
                     /locus_tag="AC159P_14785"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006636 - unsaturated fatty acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HIR3696765.1"
                     /translation="MADKAVLNLSRTGVNWATAITMVVFHAASVAALFMFTWEGLGAF
                     LLLTWVAGSLGIGMGYHRLLTHRGYKTPKAVEYFLTLCGTLALEAGAINWVATHRIHH
                     SHTDAPGDPHTPRDGTWWAHMGWILTGTAQQYDEETLRRYAPDLMKDPVHVFLNRFFY
                     LPVVALGLLLFWFGGWSVLLWGLFLRTTLGLHATWLVNSATHLWGTRRFETTDDSTNT
                     WLVALITFGEGWHNNHHAHPRAARHGVAWYELDINWIGIRTLQLVGLAKDIKLIKAAA
                     EAPRAAEEQPPVALREAA"
     gene            2477..3391
                     /locus_tag="AC159P_14790"
     CDS             2477..3391
                     /locus_tag="AC159P_14790"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3696766.1"
                     /translation="MRISSRRKSIAFFITLGAVLVAVAVALNVTWILVSWQKVALLIL
                     GIILFVVIITGLVLNTIFLVREIRRNEQHDSFINAVTHELKTPITSIKLYLETLKTRD
                     VDEEQRRQFYDVMLADSDRLLHTVEQVLRAGRTGHRRRQIQNSLIDIGQMTREEVELA
                     RSRYHLDERALRYAEAVAGDRAFRVRGDADELRAAVSNLLDNAVKYSHKEVDVQVSVE
                     AAEGARGVAVRVRDRGVGIGRGQAKRIFNRFYRAPGRVMARVKGTGLGLFIVRSVVKK
                     HGGQVFAESDGPGRGSTFTILLPRGDQR"
     gene            3388..4149
                     /locus_tag="AC159P_14795"
     CDS             3388..4149
                     /locus_tag="AC159P_14795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR3696767.1"
                     /translation="MSAAVLIVEDEKHLADGLRFNLEAEGYRVETVADGEAALDVLLR
                     GRQGFDAVVLDVMLPGVDGFEVASQLRRAGQFVPVLMLTARGRPEDVLRGFESGADDY
                     LPKPFELTILTARLRGLIRRREWFTRDLETATPAASDAAPVAGGTADVFTFGGKTIDF
                     GALELRAKGRTVRLTLMEADLLRYLVRHAGRAVSRRGVLEDVWNLDEETDTRAIDNFV
                     VRLRKYIEDEPSQPRHLLTVRGVGYRFVPEPADAA"
     gene            4372..>5707
                     /locus_tag="AC159P_14800"
     CDS             4372..>5707
                     /locus_tag="AC159P_14800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HIR3696768.1"
                     /translation="MTAGTFRRNGKPKDAPRAADEGAARPEPPADGGRLIVLTAPLTE
                     AIDHAGYFIQMAMASLPVWMEGVINRKYPKWREVERNADGTARYMPAGVRLVEKSLQR
                     AYDAGDIVACYPEDLNRFVGPRTRVVAVSTHNPLGVTFAAGVYTSIFGSSRMPINSHY
                     SRELFAAIKSSPYRQNFKVIVGGSGGWQIIQTDTFEELSVDCVVEGRSESAETLELFR
                     KAVEGEALPRTVEVKHPNDPAQILFPDARTTFGVVEMTTGCGRRCKFCVPDLNPQISL
                     PKDRIMEAVRANVRQGNKQISLATEDMFIWGQVHTDTPFYFPNREALLDLYGEIVNTP
                     GVEQHVLSHSTIAPAVVDPILIKELSRLLLPKSPIHFPYLSSHPEKKALAPLIGLETG
                     SIRTAKQIMSSKGVPFHVEDWPSVVLEGLRVLNENNWFPAMTLIVGNPGERDE"
BASE COUNT          951 a         2013 c         1780 g          963 t
ORIGIN      
        1 aggcgaaggt cgagaagaac tggctcgaat ggatcgtctt cggcgtcggc ctcgtgctcg
       61 tcgcgggcgt gctcggcttc ctcgtctacg acggcgcgcg catgggccag gcgcctcccg
      121 acatcgagtt gaagctcggc gcgcccgagc ggcgcggcgc ggggttcgtc gtccccgtcg
      181 ccgccaccaa ccgcggcgac gagaccgccg aaggcgtcca gatcgaagtc gtgctggagg
      241 ccggcggcgg cgaacccgag cgcggcgaat tcgccatcgc cttcctcccc cgccgcgcca
      301 cccgccacgg ctgggtcacc ttccgcaccg accccggcac aggccaactc aaggcccgcg
      361 tcctcggcta cgaaaaacct tagcggcacg aacccgcgcg gcattgggcc gcagcggtct
      421 ctaccattca gcgcgacggc ggcctatata tcagtcaggc gatccacatt atggatataa
      481 aatcttggag ccaagccaaa gctgaacttg cggtagatgc tttggttgat gccgggctgg
      541 tcagagcaga ggatttcgaa cgagcggtag ccatcgtggc ggaggagatt cttgtgcggc
      601 tgcttctcgg tgattatccg cctcccgaag agtcgcagac accagaccct acagcccgac
      661 aacgcattca accggagcgc gaatagcgtt gttttcatcc gcgagacttg gatgctcctg
      721 cgtcgaatgc gcggcggatg acgccgtgcg tcatgctttt ttccatccgt aagcagtggg
      781 aggattcatg gatttgactc caatgatcgc gcttcttctc ggcgccgtcc tctcgcttgc
      841 cctcgttggc gcggcacgca tgttcgcgcc gggaagggaa aggctcgtct atgccgtcgg
      901 cctcgtgctc acggccctcg tctacgtcgt cttcgcggtg gccggcgggg cgagcgcgcg
      961 gtgggcggcg attgaaagtc tcggcgtcct tctctacgga gccgcggctt ggggcgggct
     1021 tcggggcctg ctccggttac tcgcggcggg ctgggccgcg cacgtcgcct gggatgtact
     1081 attgcacctg agcggcgccg gagccgagta cacgccgcat tggtatccgt ggctctgtgt
     1141 cagcttcgat ctgatcatgg cgggcgccgt gctggcgtcg agcaggcggg gggccgcgga
     1201 cttgcgcggc atagcatgac aacggcccgc ccgaccccgc ccgccccgca gtaacacccc
     1261 gcccccgaga gttcgcgtga tgttttcgtc accgaaacat cacgaacggt caggcccgcc
     1321 acgccgcccc ccacgttctt acataactat tacaatccgc ccgcgcgtct cctgctagga
     1381 ttcttctcac ccccgcattt catactttcg aacgttttct gttggactac ggaggagcac
     1441 gatggctgac aaggcggtgt tgaacctgag ccggacgggc gtcaactggg cgacggcgat
     1501 cacgatggtt gttttccacg cggcgtccgt cgccgcgctg ttcatgttca cgtgggaggg
     1561 cttgggcgcc ttcctcctgc tgacgtgggt cgcgggcagc ctcggcatcg gcatgggcta
     1621 ccaccgcctg ctgacgcacc gcggctacaa gacgccgaag gccgtcgagt atttcctcac
     1681 cctctgcggc acgctcgcgc tcgaagccgg cgccatcaac tgggtcgcca cgcaccgcat
     1741 ccaccactcg cacaccgacg cgcccggcga cccgcacacg ccgcgcgacg gcacgtggtg
     1801 ggcgcacatg ggctggattc tgacgggcac cgcgcagcag tacgacgaag agaccttgcg
     1861 ccgctacgcg cccgatctga tgaaagaccc cgtgcacgtc ttcctcaacc gcttcttcta
     1921 cctgccggtc gtcgccctcg gcctgctgct cttctggttc ggcggctggt cggtgctgtt
     1981 gtggggcctc ttcctgcgca cgacgctcgg cctgcacgcg acgtggctcg tcaactccgc
     2041 gacgcacctg tggggcaccc gccgcttcga gaccacggac gactcgacca acacctggct
     2101 cgtcgcgctc atcaccttcg gcgagggctg gcacaacaac caccacgcgc acccgcgcgc
     2161 cgcgcgccac ggcgtcgcct ggtacgagct ggacatcaac tggatcggca tccgcacgct
     2221 gcaactcgtc ggcctcgcca aagacatcaa actcatcaag gccgccgccg aagcgccccg
     2281 cgccgccgaa gagcagccgc cggtcgcgct ccgcgaagca gcctaagacc gaaggggaaa
     2341 aggttaaaag gggaaaaggc gaaaagggaa agacaaacat cttccttttc gcctttcgcc
     2401 ttttcccctc ttccccttct cccttttcgc cttttcccct tttaaccttt tcccccttcc
     2461 ctcgtacaat gtgctcatgc gcatcagcag ccgccgcaaa tccatcgcgt tcttcatcac
     2521 gctcggcgcg gtgctggtgg cggtcgccgt cgcgctcaac gtgacgtgga ttctggtgag
     2581 ctggcagaag gtcgcgctgc tcatcctcgg catcatcctc ttcgtcgtca tcatcacggg
     2641 actggtgctc aacaccatct tcctggtgcg cgaaatccgc cgcaacgagc agcacgactc
     2701 cttcatcaat gccgtcacgc acgagctgaa gacgcccatc acctcgatca agctctacct
     2761 cgaaacgctg aagacgcgcg acgtggacga ggagcagcgc cgccagttct acgacgtgat
     2821 gctcgccgac agcgaccgcc tcctgcacac cgtcgagcaa gtcctgcgcg cggggcgcac
     2881 gggccaccgc cgccgccaga tacagaattc gctcatcgac atcggccaga tgacccgcga
     2941 ggaggtcgag ctggcgcgct cgcgctacca cctcgacgag cgcgcgctcc gctacgccga
     3001 ggccgtggcg ggcgaccgtg ccttccgcgt gcgcggcgac gcggacgagc tgcgcgcggc
     3061 ggtctcgaac ctgctggaca acgcggtcaa gtattcgcac aaggaggtgg acgtgcaggt
     3121 ctccgtcgag gccgccgagg gcgcgcgcgg cgtcgcggtg cgcgtccgcg accgcggcgt
     3181 cggcatcggg cgcgggcagg ccaagcgcat cttcaaccgc ttctaccgcg cgccgggccg
     3241 cgtcatggcg cgggtgaagg gcacgggcct cggcctcttc atcgtgcgct cggtggtgaa
     3301 gaagcacggc gggcaggtct tcgccgagag cgacgggccg gggcgcggct cgacgttcac
     3361 gatcctgctg ccgaggggcg accagagatg agcgcggcgg tgctgatagt cgaggacgag
     3421 aagcacctgg cggacggcct gcgcttcaac ctcgaagccg agggctaccg cgtcgagacg
     3481 gtcgccgacg gcgaggcggc gctcgacgtt ctcctgcgcg gacggcaagg cttcgacgcc
     3541 gtcgtgctcg acgtgatgct gccgggcgtt gacggcttcg aggtcgcctc gcagctccgc
     3601 cgcgcgggcc agttcgtgcc ggtgctgatg ctgacggcgc gcggtcgccc cgaagacgtg
     3661 ctgcgcggct tcgagtcggg cgccgacgac tacctgccga agcccttcga gctgacgatc
     3721 ctgacggccc gcctgcgcgg cctcatccgc cgccgcgagt ggttcacgcg cgacctcgaa
     3781 accgcgacgc ccgccgcgag cgacgccgcg cccgtcgcgg gcggcaccgc agacgtgttc
     3841 accttcggcg gcaagaccat cgacttcggc gcgctggagc tgcgcgcgaa ggggcgcacc
     3901 gtccgcctca cgctgatgga ggccgacctg ctgcgctacc tcgtccgcca cgcgggtcgc
     3961 gccgtctcgc gccgcggcgt cctcgaggac gtgtggaacc tcgacgagga gaccgacacg
     4021 cgcgccatcg acaacttcgt cgtccgcctg cgcaagtaca tcgaggacga gccttcgcag
     4081 ccgcgccacc tcttgaccgt ccgcggcgtc ggctaccgct tcgtccccga acccgccgac
     4141 gccgcttaga gcggttccgc acacttcttc cttgacttcg atttcccgaa agcattattt
     4201 tgccgtccat cttgccggtc gctctaacct ttgaatcgcc ggagaccttc ggccacgcgg
     4261 caagccatcc cgctcgacga tcccggccac aagctcttac cctcgcggga gacagtccag
     4321 agcacttttt tgccgcggca gcgacgcggc ctcgtggagg attaattaca gatgaccgca
     4381 gggacattcc gccggaacgg aaaaccgaaa gacgcgccgc gcgccgctga cgagggcgcc
     4441 gcgcgtccgg agccgcccgc cgacggcggc aggctcatcg tgctcacggc cccgctcacc
     4501 gaagcaatcg accacgcggg ctacttcatt cagatggcga tggcctcgct gcccgtctgg
     4561 atggagggcg tgatcaaccg caagtacccg aagtggcgcg aggtcgagcg caacgcggac
     4621 ggcacggcgc gctacatgcc cgcgggcgtg cggctcgtcg agaagtcttt gcagcgcgcc
     4681 tacgacgcgg gcgacatcgt cgcctgctac ccggaagact tgaacagatt cgtcggcccg
     4741 cgcacgcgcg tcgtcgccgt ctcgacgcac aacccgctcg gcgtcacctt cgccgcaggg
     4801 gtttacactt ccatcttcgg ctcgtcgcgc atgcccatca actcgcacta ctcgcgcgag
     4861 ctgttcgccg ccatcaagtc gagtccgtac agacagaact tcaaggtcat cgtcggcggc
     4921 tccggcggct ggcagatcat ccagaccgac acgttcgaag agctgagcgt cgattgcgtg
     4981 gtcgagggcc ggagcgagtc cgccgagacg ctggagctgt tccgcaaggc cgtcgaaggc
     5041 gaagccttgc cgcgcaccgt cgaggtcaag cacccgaacg accccgcgca gattctcttc
     5101 ccggacgcgc gcaccacctt cggcgtcgtc gagatgacga cgggctgcgg tcgccgctgc
     5161 aagttctgcg tccccgactt gaacccgcag atatcgctcc ccaaggacag gatcatggag
     5221 gccgtgcgcg ccaacgtccg gcagggcaac aagcagattt cgctcgccac cgaggacatg
     5281 ttcatctggg ggcaggtgca caccgacacg cccttctact tccccaaccg ggaagccctg
     5341 ctcgacctct acggcgagat cgtcaacacg cccggcgtcg agcagcacgt cctctcgcac
     5401 tcgaccatcg ccccggcggt cgtcgatccg atactcatca aagagctgtc gcggctgctc
     5461 ctgccgaaaa gcccgatcca cttcccctac ctcagcagcc accctgagaa gaaggcgctc
     5521 gcgccgctca tcggactcga aaccggctcc atcaggacgg ccaagcagat catgtcttcg
     5581 aagggcgtgc cgttccacgt cgaggactgg ccgagcgtcg tcctcgaagg cttgcgcgtg
     5641 ctgaacgaga acaactggtt ccccgcgatg acgctcatcg tcggcaaccc cggcgaacgg
     5701 gatgagg
//
LOCUS       DBNGOH010000767         2999 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_5064403, whole genome shotgun sequence.
ACCESSION   DBNGOH010000767 DBNGOH010000000
VERSION     DBNGOH010000767.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2999)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2999)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2999
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_5064403"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..660)
                     /locus_tag="AC159P_14805"
     CDS             complement(<1..660)
                     /locus_tag="AC159P_14805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696769.1"
                     /translation="MCNRKDGRAGPSAGFSLIELIIALGVTLVIMVISAQLLAASLNV
                     RTRENARAEAIADVQRGLQTMSREIGNAGLGLVVNGQAVNGLHPTDSDEAQIRVRANL
                     NAFEGDADTLDTLDAGSEDVVFAIINNFTGGEPEGQRLITRQDVNTQRISSLANRVDS
                     LRFDYLNADGTVAAAPAQAARVRITVGVTLRAVGTPGRPGYQPQSRTQVVSDVSLRNR
                     LL"
     gene            complement(693..1163)
                     /locus_tag="AC159P_14810"
     CDS             complement(693..1163)
                     /locus_tag="AC159P_14810"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus modification PilV family protein"
                     /protein_id="HIR3696770.1"
                     /translation="MNSMQHTDARARRPAPEGSRGFTLLETVIALAVMLVVGFGAVSL
                     FLFSVNYNSGASDRARALAIAQQRIEALRSAPFTGLANGDTTANVNAGSTAAGESDRR
                     TFAVRTVIETTGGVNNSRQKTITVTVTPNATGGRMSIGGVMLRTYRSSTEFVVP"
     gene            complement(1179..2294)
                     /locus_tag="AC159P_14815"
     CDS             complement(1179..2294)
                     /locus_tag="AC159P_14815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly FimT family protein"
                     /protein_id="HIR3696771.1"
                     /translation="MAVRSTKRKGEAGFSLVQMVVTLAVVAIVTTFATMRVVVARDHM
                     RLNNAAREFAAVAEKARIDSVRRHENGGQATLFISSPTTYLVFMDHNYSGNPAWRTYS
                     LPDGVQFANLKVTQANGTVENSAAFPVTVSFSWRGHSSTANYITFQNTHGHSSFVSIT
                     GAGEVSLDRGEISLLAGIYAAVAGDASALGPGVNPGGGTGIEGGAPPPPPTPPPSPTP
                     SPSPEPTASPTPQPSASPTPQPSATPSAEPSATPSASPTPQPSASPTPTPEATPVPTP
                     PCTMSLSPTRLDLHNSGNNQPQTGVVTVTFANPTANPTFTVKEVSSTEHLTISFTANK
                     ITIGAKNGGGNRGAFLVRVTPSTGCGSAQDINVNVAQ"
BASE COUNT          424 a          941 c         1155 g          479 t
ORIGIN      
        1 gaggaggcgg ttgcgcagcg acacgtccga gacgacctgc gtccgcgact gcggctggta
       61 gccggggcgg cccggcgtgc cgaccgcgcg caacgtgacg ccgacggtga tgcggacgcg
      121 cgcggcctgc gcgggcgcgg cggcgaccgt gccgtccgca ttcaggtagt cgaaccggag
      181 gctgtccacg cggttggcga gcgaggagat ccgttgcgtg ttcacgtcct gccgcgtgat
      241 gagccgctgc ccctccggct cgccgccggt gaagttgttg atgatggcga agaccacgtc
      301 ctcggagccg gcgtcgaggg tgtcgagcgt gtcggcgtcg ccctcgaagg cgttgaggtt
      361 ggcgcggacg cgaatctgcg cctcgtccga gtcggtgggg tgcagcccgt tgaccgcctg
      421 cccgttgacc accagcccga ggccggcgtt gccgatctcg cgcgacatcg tctggagacc
      481 gcgctgcacg tcggcgatgg cctcggcgcg cgcgttctcg cgcgtgcgga cgttgagcga
      541 ggcggccaag agctgggccg agatgaccat gatgaccagc gtcacgccga gcgcgattat
      601 cagctcgatg agcgagaagc ccgcgctcgg gccggcccgc ccgtccttcc tgttacacat
      661 cggcaagtct ccttcgaaag gcgcgggccg cgctagggca cgacgaactc ggtcgaagag
      721 cggtaggttc tgagcatgac gccgccgatg gacatgcgcc cgccggtggc gttgggcgtc
      781 acggtgacgg tgatcgtctt ctgccgcgag ttgttgacgc ccccggtggt ctcgatcacg
      841 gtcctgacgg cgaaggtgcg gcggtcggac tcgcccgccg ccgtgctgcc ggcgttgacg
      901 ttcgccgtcg tgtcgccgtt ggccagcccg gtgaagggcg cgctgcgcag cgcctcgatc
      961 cgctgctgcg cgatggcgag ggcgcgcgcg cggtcgctcg ccccggagtt gtagttgacc
     1021 gagaagagga agagcgagac ggcgccgaag ccgacgacca gcatgaccgc cagcgcgatg
     1081 accgtctcca gcagggtgaa gccgcggctg ccttccgggg cggggcgccg cgcgcgcgcg
     1141 tccgtgtgtt gcatcgagtt cattcgcttc acacccctct actgcgcgac gttgacgttg
     1201 atgtcctgcg cgctgccgca gcccgtgctc ggcgtgacgc gcacgaggaa ggcgccgcgg
     1261 ttgccgccgc cgttcttggc gccgatggtg atcttgttgg ccgtgaagga gatcgtcagg
     1321 tgctcggtgc tgctgacttc tttgacggtg aacgtggggt tcgccgtggg gttggcgaag
     1381 gtcacggtca cgacgccggt ctgcggctgg ttgttcccgc tgttgtgcag gtcgagcctc
     1441 gtcggcgaca gcgacatggt gcacgggggc gtcggcaccg gcgtcgcctc cggggtcggc
     1501 gtcggggacg cggacggctg cggcgtcggg gacgcggacg gggtcgcgga cggctccgcg
     1561 gatggggtcg cggacggctg cggcgtgggc gaggcgctcg gctgcggcgt gggcgaggcg
     1621 gtcggttcgg gcgacggcga gggcgtgggt gacggcgggg gcgtgggcgg gggcggcggg
     1681 gcgccgcctt cgatgccggt gccgccgccg gggttgacgc cggggccgag ggccgaggcg
     1741 tcgccggcga cggcggcgta gatgcccgcg agcagcgaaa tctcgccgcg gtcgagcgag
     1801 acctcgcccg cgccggtgat gctgacgaag ctggagtggc cgtgcgtgtt ctggaaggtg
     1861 atgtagttgg ccgtgctgct gtggccgcgc caactgaagg agaccgtgac cgggaaggcg
     1921 gcgctgttct cgaccgtccc gttggcctgc gtgaccttga ggttggcgaa ctggacgccg
     1981 tccggcagcg agtaggtgcg ccaggccggg ttgcccgagt agttgtgatc catgaagacg
     2041 aggtaggtcg tcgggctgct gatgaacagc gtcgcctgcc cgccgttctc gtgccggcgc
     2101 acggagtcga tgcgcgcctt ctcggcgacg gcggcgaact cacgcgcggc gttgttgagc
     2161 cgcatgtggt cgcgcgcgac gacgacgcgc atggtggcga acgtcgtcac gatagccacc
     2221 acggccaacg tgacgaccat ctgcaccaac gagaatcccg cctcgccctt cctcttcgta
     2281 cttctcacgg ccatcgtctg cctcccacct cgacttccgc ctgacggtcg cccgagcgcg
     2341 atgaaaaccg cacgcgcctc cctcggcagg gcaacgcggg tgccacgacg agtgcgggcc
     2401 gaaatgctgg acgttcgcgc cgacgtgcga cggggcgccg cggcgtgagc gtcactttag
     2461 gtgacgagtt gagaagccgc cttcacagcc gcggaggccg tcgatccgtc gcgtgagctg
     2521 ccccgcgccg cgcccggtat agtcgatgcg gcggacgcac cgccgttaca caacttgtta
     2581 ccgacagaaa caagtggcgc gtgccggcga agccgactgc ggcttcggtc gcgtgggccg
     2641 tgggcgacgc aagctgtaag cgagaaagag gcttgcggtg tttcgcggat gaagggcggg
     2701 ggcgcggtgt gcgagttgct gtagagggag gcggcctttc gtggccgatc aaacttgcct
     2761 tcggcgggac gcggtgcggc gtacttcaca ggcaaagacc ccggaagcaa cacgtcaacc
     2821 cccacaagat cgtcccctcg cgtgcggctc cgcacagccg cgtgcgtgcg ggacgaaaag
     2881 cgccccggcg gcttcacggc tgccggggcg cgaattttta gaagggggga aagggagaag
     2941 gggagaaagg gagacgggga gtgggggaga tgaagacacg ccgtctcatc tcccccact
//
LOCUS       DBNGOH010000768         2101 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1216944, whole genome shotgun sequence.
ACCESSION   DBNGOH010000768 DBNGOH010000000
VERSION     DBNGOH010000768.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2101)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1216944"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(265..2019)
                     /locus_tag="AC159P_14820"
     CDS             complement(265..2019)
                     /locus_tag="AC159P_14820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019119300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HIR3696772.1"
                     /translation="MRNLREFLDLLRREGDLVEVGAEVDPDLELAEVHRRVIEREGPA
                     LLFNRVKGSRYPVVTNLFGSERRIGWAFGPKPEALVRQAVHVAESLMPPRPSELWRQK
                     SLALEALRLGTRRVRRAPVLEVSDRPARLDELPILTTWQEDGGPFVTLPLVYTEHPAT
                     GKHNLGMYRLQRYDAATTGMHWQIHKGGGFHYHEAEKEGRALPVTVFLGGPPALILSA
                     IAPLPEDVPELVLASVLAGEKIRVADNPLEGHTHRLVAEAEFALAGRVPPRERRPEGP
                     FGDHYGYYSLQHDYPVFRVEAVFHRKDAIYPATVVGKPRQEDFFIGDYLQKLLSPLFP
                     LVMPSVRDLWTYGETGFHSLCAAVVRERYAREALVSGFRILGEGQLSLTKFLILTDTP
                     QDLKDFKSLFEHVLARVRFESDLFVFSNVSMDTLDYTSGKVNEGSKAIMLGLGDAVRE
                     LPREFAGTLPAGVARAESFCGGCLVVEGVPYEQEPAQAARLARDAAFSDWPLVVLHDD
                     ARVARSAPDFLWATWTRFEPAADIHAAATQVNRHHISYSAPLVIDARMKPGYPDELVV
                     RPDIAELVDRRWREYFPK"
BASE COUNT          348 a          660 c          748 g          345 t
ORIGIN      
        1 tgctcaagag aaagaacacg cggcggcgca cacgacggat gcctgaagta agactcgcta
       61 tggtgacggc ttagggcgga ctgcggttga gtttacccga ggcagggttt aacgcagagg
      121 acgcggaggg acgcagaggg cgcagagaaa gagatcgctt cttctctgcg ccctctgcgc
      181 ccctccgcga accctgcgtt gaaccggacg cgcgtttcgc tcaactgctc atcctgtatt
      241 tcgaatcgtt gtagccctca tctcttactt cggaaaatac tcacgccacc ttcggtcaac
      301 cagttccgcg atgtcggggc ggacgaccaa ctcgtccggg tagccgggtt tcatgcgcgc
      361 gtcgatgacg agaggcgcgc tgtaggagat gtggtggcgg ttgacttggg tggcggcggc
      421 gtggatgtcc gccgcaggct cgaagcgcgt ccaggtcgcc cagaggaagt ccggcgcgga
      481 gcgggcgacg cgcgcgtcgt cgtggagcac gacgagcggc cagtcggaga acgccgcgtc
      541 gcgtgcgagg cgtgccgcct gcgcaggctc ctgctcgtaa ggcacgccct cgacgacgag
      601 gcagccgccg cagaagcttt cggcccgcgc gaccccggcg ggcagcgtcc ccgcgaactc
      661 gcgcggcagc tcgcgcaccg cgtcgccgag gccgagcatg atcgccttgc tgccttcgtt
      721 caccttcccg ctcgtgtagt cgagcgtgtc catcgagacg ttggagaaga cgaagaggtc
      781 gctttcgaaa cggacgcgcg cgagcacgtg ctcgaagaga ctcttgaaat ctttcaagtc
      841 ctgcggcgtg tcggtgagga tcaggaactt ggtgagcgac agttggcctt cgccgaggat
      901 gcggaagccg gagaccagcg cctcgcgcgc atagcgctcg cgcacgaccg ccgcgcagag
      961 cgagtggaag ccggtctcgc cgtacgtcca gaggtcgcgc acgctcggca tcacgagcgg
     1021 gaagagcggc gacaggagtt tctgcaagta gtcgccgatg aagaagtcct cctgccgcgg
     1081 cttgccgacg acggtcgccg ggtagatcgc gtccttgcga tggaagaccg cctcgacgcg
     1141 gaagaccggg taatcgtgtt gcagcgaata atatccgtag tgatcgccga aagggccttc
     1201 gggtctgcgc tcgcgcggcg ggacgcggcc cgcgagcgcg aactccgctt ccgcgacgag
     1261 gcggtgcgtg tggccttcga gcgggttgtc ggcgacgcgt atcttctcgc ccgcaaggac
     1321 ggaggcgagg actagctcgg gcacgtcctc gggcagcggc gcgatggccg agaggatgag
     1381 cgcgggcggg ccgccgagga agaccgtgac gggcagcgcc cgtccttcct tctccgcctc
     1441 gtgatagtgg aagccgccgc ccttgtgaat ctgccagtgc atgcccgtgg tcgcggcgtc
     1501 gtagcgctgc aagcggtaca tgccgaggtt gtgtttgccg gtcgcggggt gctccgtgta
     1561 gacgagcggc agcgtgacga aggggccgcc gtcctcctgc cacgtcgtca ggatcggcag
     1621 ctcgtccaga cgcgcgggcc tgtccgagac ttcgagcacg ggcgcgcggc gcacgcggcg
     1681 cgtgccgagc cgcagcgctt cgagcgccag agacttctgc cgccacagct ccgaggggcg
     1741 cggcggcatg agcgactcgg cgacgtggac ggcctgtcgg acgagcgcct cgggcttcgg
     1801 gccgaaggcc cagccgatgc gacgctcgga gccgaagagg ttcgtgacga cggggtagcg
     1861 gctccccttc actctgttga agaggagcgc cggcccctcg cgctcgatga cgcggcggtg
     1921 gacttcggca agttcgaggt cggggtcgac ttccgccccg acctcgacga ggtcgccctc
     1981 gcggcgcagc aggtcgagaa actctctcag gttacgcacg cgaggaattc tatccacgaa
     2041 gggccacgaa ggaccacgaa gcttcgtgtc cttcgtggcc cttcgtggct acgctttttg
     2101 c
//
LOCUS       DBNGOH010000769         2278 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4880634, whole genome shotgun sequence.
ACCESSION   DBNGOH010000769 DBNGOH010000000
VERSION     DBNGOH010000769.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2278)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2278)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2278
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4880634"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            313..1383
                     /gene="mreB"
                     /locus_tag="AC159P_14825"
     CDS             313..1383
                     /gene="mreB"
                     /locus_tag="AC159P_14825"
                     /inference="COORDINATES: protein motif:HMM:NF010539.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HIR3696773.1"
                     /translation="MNILGSIFTDDLAIDLGTVNTLIYAPDSGIVLQEPTAVAIHKYT
                     GEVLSVGAEAYKMLGREPKDTEVFRPIRGGTIDNFEAAEKMLKAFITRVTGSQMRRSH
                     MVVGVPGSSTPIEQRSVRDAARDAKATRVDLVDEGLCAALGAGLSHEDERAHLVVDIG
                     GGTTNIAIIASGAVVSSASLLAAGNAMDEAIRDYVRSKYEMQLGERAAEQLKKELSAG
                     GDADQGAERRMEVVGKQVTDGLAKAVEISAAEVREALEPVISEIIAGARRAIEESKPD
                     VTADIYQTGLILTGGGSLLEGMAARLQKELRLHVAHAEDPLAAVARGAGGLLTNKAKL
                     NRVAIREDVPVWQAQEELVVNW"
     gene            1626..>2278
                     /locus_tag="AC159P_14830"
     CDS             1626..>2278
                     /locus_tag="AC159P_14830"
                     /inference="COORDINATES: protein motif:HMM:NF012707.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HIR3696774.1"
                     /translation="MRGHARLLAPVLLLLSLLVLPDCKRKPPEEAAVDPIIRAADVPM
                     PEPVARDLAELRERGTLTVLAPYNSTTYFLYRGEPLGYEYELLREFAREHGLALKMVV
                     VADRKSLYPLLNGGDGDVAAGRLIPTPEDERHVAFTRELYRTEPALVQQAEPPQEANL
                     PGDAKKALAPGPAEPAPEADIQARLVTRPAQLAGKRVDLPEQHPYRRTLVELEDDISG
                     "
BASE COUNT          393 a          789 c          757 g          339 t
ORIGIN      
        1 agcgcgcggc ggtcgggggt ttcagtcgaa acagaggttg ctttttgact gacccctgac
       61 cgcccgccgc cgccgacttt tccacagccg ttcgcctttc gcgcggcgcg agtaattttc
      121 cacgccggtt tgtcttgaat cgagacgcga cgcggcggcg gcgaaaatgg aagaccgggg
      181 gaaatcctgc tttcctttac aggaacgaca cacgagccac actggaacga tgtttgcccc
      241 ttgagtgacg aacatctcag cccggccctc cccgcgcgag actttctgga aggcagaggg
      301 gaaaggggtt ttatgaacat ccttggtagc atctttacgg acgatctcgc catcgacctc
      361 ggcaccgtca acacactcat ctacgcgccc gacagcggca tcgtcctgca agagccgacg
      421 gcggtcgcca tccacaagta cacgggcgaa gtgctctcgg tcggggccga agcctacaag
      481 atgctgggtc gagagccgaa ggacaccgaa gtcttccgcc ccatccgcgg cggcaccatc
      541 gacaacttcg aggccgccga gaagatgctc aaggccttca tcacgcgcgt caccgggagc
      601 cagatgcggc gcagccacat ggtggtgggc gtgcccggct cttcgacccc catcgagcag
      661 cgctccgtga gggacgcggc gcgcgacgcg aaggccacgc gcgtcgatct ggttgacgaa
      721 ggcttgtgcg ccgcgctcgg cgccggcctc tctcacgaag acgagcgcgc ccacctcgtc
      781 gtggacatcg gcggcggcac caccaacatc gccatcatcg cctcgggcgc ggtcgtttcc
      841 tcggcgagcc tgctcgcggc gggcaacgcg atggacgagg ccatccgcga ctacgtgcgc
      901 tcgaagtacg agatgcagct cggcgagcgc gccgccgagc agttgaagaa ggagctgagc
      961 gcgggcggcg acgccgacca aggggcggag cgccggatgg aggtcgtcgg caagcaggtg
     1021 acggacgggc tggcgaaggc cgtcgagatt tccgccgcgg aggtgcgcga ggcgctggag
     1081 ccggtcatct cggagatcat cgcgggcgcg cgccgcgcca tcgaggagtc gaagcccgac
     1141 gtgaccgccg acatctacca gacgggcctc atcctgacgg gcggcggctc gctcctcgaa
     1201 ggcatggccg cgcgcttgca gaaggagctg cggctgcacg tcgcgcacgc cgaagacccg
     1261 ctcgcggcgg tcgcgcgcgg cgccggcggt ctcctgacca acaaggcgaa gctcaaccgc
     1321 gtcgccatcc gcgaggacgt gcccgtctgg caggcgcagg aggagttggt cgtcaactgg
     1381 taacacgcgg gtcatcgtcg agccatcgtt tgaaactacg cgccgtggca aaactgtagg
     1441 ggcagggctt gtccctgcct gccgcgtccg accgattcaa cgtcgcgtgt gattgtgact
     1501 gcgggccgcg gacttgccct gcccctacaa gcatcacgcg cgtcgagagt atcaacccaa
     1561 gccgcagtct ttccgccgac ccccacacaa gcagtccttc ctccccgcgt tcggagttct
     1621 ctgccatgag aggccacgca cgtctgcttg cgcccgtcct gctgctgctc tctcttctcg
     1681 tcctgcccga ctgcaaacga aagccgcccg aagaggcggc ggtggacccg atcatccgcg
     1741 cggctgacgt gccgatgccg gagccggtcg cgcgcgacct cgccgagcta cgcgagcggg
     1801 ggacgctgac cgtgctcgcg ccgtacaact cgaccaccta ttttctctac cgcggcgagc
     1861 cgctcggcta cgaatacgag ctgctgcgcg agttcgccag ggagcacggg ctggcgttga
     1921 agatggtggt ggtggcggac aggaagagtc tctacccgct gctgaacggc ggcgacggcg
     1981 acgtggccgc gggccgcctg atcccgacgc cggaggacga gcgccacgtc gccttcacgc
     2041 gcgagctgta ccgcaccgaa cccgcgctcg tgcagcaggc cgagccgccg caggaggcga
     2101 acctgcccgg cgacgcgaag aaggcgctcg cgcccggccc cgccgagcct gcgcccgagg
     2161 ccgacatcca ggcccgcctc gtcacgcggc ccgcgcagct cgcgggcaag cgcgtcgatc
     2221 tgcccgagca gcacccctac cgccgcacgc tcgtcgagct tgaggacgac atctcggg
//
LOCUS       DBNGOH010000770         2733 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_299696, whole genome shotgun sequence.
ACCESSION   DBNGOH010000770 DBNGOH010000000
VERSION     DBNGOH010000770.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2733)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2733)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2733
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_299696"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1343)
                     /locus_tag="AC159P_14835"
     CDS             complement(<1..1343)
                     /locus_tag="AC159P_14835"
                     /inference="COORDINATES: protein motif:HMM:NF042664.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing protein"
                     /protein_id="HIR3696775.1"
                     /translation="MQETATPYALEASRILDHVAKSRNGGQNGNGDGCVQAKPLDGAH
                     LKKVEQGASREGRGELAREMRRLRIEADGKQAERILGGIAATPAEMLKLAKRLAEYKK
                     FGLARRVLRRARLQSNLTPSADIYVEVYQKSALYTYKDPDLPLEWRLQRALEILSALE
                     DLSKTRNQETLGLAGAIHTRMWQVGGQRKHLWRALGFYLRGYEQGAPTEAHGQLFTYL
                     RRNPECVFEPNDDRGYTGINTAFVLDMLAREEEEDADSVGLVSEAAKDKREFARLIRR
                     EIVRSVSPLAGNPGGEWLAGEWWYYATVGEAHFGLGQYEEAVEWLTAQPGAAGLKIAV
                     GETTPAGLEVPEWEFETTARQLGALARLTRPRREPAMSDEEFCRHTADEFRKAQAALK
                     VILGGDEKAVASAFRGKFGLGLSGGGFRASLYHIGVLAKLAELDVLRHVEVLSCVS"
     gene            complement(1518..2465)
                     /locus_tag="AC159P_14840"
     CDS             complement(1518..2465)
                     /locus_tag="AC159P_14840"
                     /EC_number="4.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012904.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0071704 - obsolete organic substance
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HIR3696776.1"
                     /translation="METHDQTSRRTAPTARPFAEKLRGILLPFPTPFGADGEVDARAV
                     RSNIRRWNETGVSGYVALGSTGERAHLDERERAAVVEAARREIAEGLAFVVGAGEQST
                     RATVAEARRVAAQGADALLVITPHFYRAAMTAEALFRHFSEVADASPVPVVLYSIPQN
                     AGGLGVAPELAARLAAHGNVVGIKDSSGDLLNLAETLRLAGERDDFAVTIGHAGALYA
                     ALAAGARGAILAAACAVPELCVEIYARVAAGDHARALALQKLLTPFARAVTTQHGIGG
                     LKAALDLRGYTGGPVRAPLAPPDDPARREIARLLEAAAH"
BASE COUNT          369 a          982 c          962 g          420 t
ORIGIN      
        1 gagacgcacg acaggacttc gacgtggcgg agcacgtcca gctcggcgag cttggccagc
       61 acgccgatgt ggtagagcga ggcgcggaag ccgccgcccg acaggcccag cccgaacttg
      121 ccgcggaagg cgctggcgac ggccttctcg tcgccgccca ggatgacctt gagcgccgcc
      181 tgggctttac ggaactcgtc ggcggtgtgg cggcagaact cttcgtcgga catcgccggc
      241 tcgcggcgcg ggcgcgtgag acgcgcgagg gcgccgagct gccgcgcggt cgtctcgaac
      301 tcccactcgg gcacctccag cccggcgggc gtcgtctcgc cgacggcgat cttcaacccc
      361 gcggcccccg gctgcgccgt cagccactcg accgcctctt cgtactggcc gaggccgaag
      421 tgcgcctcgc cgaccgtggc gtagtaccac cactcgccgg ccagccactc gccgccgggg
      481 ttcccggcga gcggcgagac ggagcgcacg atctctctgc ggatgaggcg cgcgaactcg
      541 cgcttatcct tcgcggcctc cgagacgagg ccgacgctgt cggcgtcctc ctcctcctcg
      601 cgcgccagca tgtcgagcac gaaggccgtg ttgatgccgg tgtagccacg gtcgtcgtta
      661 ggctcgaaca cgcactcggg gttccggcgc aggtaggtga acaactggcc gtgcgcttcg
      721 gtcggcgcgc cctgctcgta gccgcgcagg tagaagccga gtgcgcgcca caggtgtttg
      781 cgctgcccgc ccacctgcca catgcgcgtg tggatcgcgc cggccagccc gagcgtctcc
      841 tggttccgtg tcttcgacag gtcttcgagc gcggagagga tttcgagcgc ccgctggagc
      901 cgccattcga gcggcaggtc ggggtctttg taggtgtaga gcgcgctctt ctggtagacc
      961 tcgacgtaga tgtccgcgct cggcgtcaga ttcgactgga ggcgcgcgcg ccggaggacg
     1021 cggcgggcca gcccgaactt cttgtactcg gcgagccgct tcgccagctt cagcatctcg
     1081 gcgggcgtcg cggcgatgcc gccgaggatg cgctcggcct gcttcccgtc ggcctcgatg
     1141 cggaggcggc gcatctcgcg cgccagctcg ccccgccctt ctctggacgc gccctgctcg
     1201 accttcttca gatgcgcgcc gtccagcggc ttcgcctgga cgcacccgtc gccgttcccg
     1261 ttctgtccgc cgttccgtga cttggccacg tggtcgagaa tccggctggc ctcaagcgcg
     1321 taaggggtgg cggtttcctg catctggacc ctccgatctg tcgtgtgggg gccggcggtg
     1381 tggccgactc gtcggcggct ggcggtcggt ctattcgcgg aaagagcgag gacgatgact
     1441 ggtaaacgac gacgcttata ccccgtcgtc ccgttgctgt caacgaaaaa tacgtgccgg
     1501 acggcgggtc gttcgggtca gtgcgcggcg gcttcgagga ggcgcgcgat ctctcggcgc
     1561 gcgggatcgt cgggcggggc gagcggggcg cgcacggggc cgccggtgta gccccgcagg
     1621 tcgagcgcgg ccttgaggcc gccgatgccg tgctgcgtcg tgacggcgcg cgcgaacggc
     1681 gtgaggagct tttgcagcgc gagcgcgcgc gcgtggtcgc ccgccgcgac gcgcgcgtag
     1741 atttcgacgc acaactccgg gacggcgcag gcagccgcga ggatcgcgcc gcgcgcgccc
     1801 gcagcgagcg ccgcgtagag cgcgcccgcg tgcccgattg tcacggcgaa gtcgtcgcgc
     1861 tcccccgcga gtctgagcgt ctccgcgagg ttgagcaggt cgcccgagct gtccttgatg
     1921 ccgacgacgt tgccgtgcgc ggcgagccgc gcggccagct cgggcgccac gcccagcccg
     1981 cccgcgttct gcgggatgct gtagaggacg accggcacgg gcgaggcgtc ggccacctcc
     2041 gaaaagtgtc tgaagagcgc ctccgccgtc atcgccgcgc ggtagaagtg cggcgtgatg
     2101 acgagcagcg cgtcggcccc ctgcgccgcc acgcgccgcg cctcggcgac ggtcgcgcgc
     2161 gtgctctgct cgcccgcgcc gacgacgaac gcgaggccct ctgcaatctc acggcgcgcc
     2221 gcctcgacca ccgccgcccg ctcccgctcg tccaggtgcg cgcgctcgcc cgtcgagccg
     2281 agcgcgacgt aaccgctgac gcccgtctcg ttccaacgtc tgatgttcga gcgaacggcg
     2341 cgcgcgtcaa cctcgccgtc cgcaccgaac ggcgtcggga acgggagcag gatgccgcgc
     2401 agcttctcgg cgaacgggcg cgcggtcggg gccgtgcgtc tgctggtttg atcgtgtgtc
     2461 tccaaagccg cggcattata gcgacccgcc gaaagcgcgc gcaaacgctt tggcgcggca
     2521 ccggcacgcc gcctcggacg taaggccacg ccggacgaca tcccggcgtc gtgcgatgat
     2581 aggcgcgtca gcgcgctcga ctcttccgcc tgcccgaaac gctttgcgct cccggcgcat
     2641 ataccttcgg aaagccgcac cgagttctcg ctgccccgac ccggacacca cccggtgccg
     2701 gtccgaccgc tgcaaaagag acggagctaa aaa
//
LOCUS       DBNGOH010000771         1771 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_115709, whole genome shotgun sequence.
ACCESSION   DBNGOH010000771 DBNGOH010000000
VERSION     DBNGOH010000771.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1771)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1771)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1771
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_115709"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="AC159P_14845"
     CDS             <1..265
                     /locus_tag="AC159P_14845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HIR3696777.1"
                     /translation="VNHVIDAESGADFMEQALSYARQFTPPDKAALAAGRIKRSVQTG
                     AEIPFESALALERELQQQLFQSDDAREGLAAYVEKRKPNFKGA"
     gene            347..1138
                     /locus_tag="AC159P_14850"
     CDS             347..1138
                     /locus_tag="AC159P_14850"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR3696778.1"
                     /translation="MYDNIKVTFDSGIATVTLDRPEKLNAFAGHMRRDLAEALEHAGG
                     ERAVRVVVITGAGRGFCAGADVKFMDELMRRDDVEEFTRLLGAGRRVLTAIRQMTKPV
                     IAAVNGPAYGAGFNLALACDLRVAAESASFSQSFVKVGLHPDWGGTYFLPRVVPSNIA
                     CEMFFLGETFDAAQALRFGIVNRVVADAELAAETRRLAERLRDAPHASIAAAKHAVYM
                     SEGSSLERMLQYETEAQIQCFQSKEARERVRAFLERREPRAAKQA"
     gene            1154..>1771
                     /locus_tag="AC159P_14855"
     CDS             1154..>1771
                     /locus_tag="AC159P_14855"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF048554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3696779.1"
                     /translation="MKLTDRIALVTGGGRGIGRAVALLFAREGARVAVVARTAGEVEQ
                     VAKEIKNAGGPDAMHATCDVSDSESVRAAFAACEGAFGRGPDILVNTAGIAESAPLVR
                     TDDELWQRLIGVNLSGVFYCTRAALPTMLGRGWGRVINVASVAAKTGAPYISAYTASK
                     HGVLGLTRAVALEVAEKGVTVNAICPGYVETDMTWGAVENITARTG"
BASE COUNT          296 a          611 c          607 g          257 t
ORIGIN      
        1 cgtcaaccac gtcatcgacg ccgagagcgg cgccgacttc atggagcagg cgctctctta
       61 cgcgcgccag ttcacgccgc cggacaaagc cgcgctcgcc gccggtcgca tcaagcgcag
      121 cgtccagacc ggcgccgaaa tccccttcga gtccgccctc gccctcgagc gcgaactcca
      181 acaacaactc ttccaatccg acgacgcgcg cgagggcctc gccgcctacg tcgagaaacg
      241 caaaccgaac ttcaaaggag cgtaaaccgt gaaccgtaaa tcgtgacaag taagaagcgg
      301 ttttctctta tcacggttca cggttcacgg ttcacggctt tcgcccatgt acgacaacat
      361 caaagtcacg ttcgacagcg gcatcgcgac ggtgacgctc gacaggcccg agaagttgaa
      421 cgcgttcgcg ggacacatgc ggcgcgatct ggcggaggct ttggagcacg cggggggcga
      481 gcgcgcggtg cgggtcgtcg tcatcacggg cgcggggcgc ggcttttgcg cgggcgccga
      541 cgtgaagttc atggacgagc tgatgcggcg cgacgacgtg gaggagttca cgcggctcct
      601 cggcgcgggc cgccgcgtgc tgacggcgat caggcagatg accaagccgg tcatcgccgc
      661 ggtcaacggc ccggcctacg gcgcgggctt caacctcgcg ctcgcgtgcg atttgcgcgt
      721 cgccgccgag tcggcctcgt tcagccagtc cttcgtcaag gtcggcctgc acccggactg
      781 gggcggcacc tactttttgc cgcgcgtcgt cccctcgaac atcgcctgcg agatgttctt
      841 cctcggcgag acgttcgacg ccgcgcaggc gctacgcttc ggcatcgtca accgcgtcgt
      901 cgcggacgcg gagctggccg ccgagacgcg gcggctcgcc gagcggctgc gcgacgcgcc
      961 gcacgcctcc atcgccgccg ccaagcacgc cgtctacatg agcgaggggt cgagcctcga
     1021 acgcatgctg caatacgaga ccgaggcgca gattcagtgc ttccagtcga aggaggcgcg
     1081 cgagcgcgtc cgcgcgtttc tggaacggcg cgagccgcgc gccgccaagc aggcttgaag
     1141 acattcgaga ccaatgaagc tgactgacag aatcgcactc gtcacgggcg gcggacgcgg
     1201 catcggtcgc gccgtcgcgc ttctcttcgc gcgcgagggc gcgcgcgtcg cggtcgtcgc
     1261 gcgcaccgcc ggcgaagtcg agcaggtggc gaaggaaatc aagaacgcgg gcgggccgga
     1321 cgcgatgcac gccacgtgcg acgtgtcgga ctcggagagc gtgcgcgccg cgttcgccgc
     1381 gtgcgaaggg gcgttcgggc gcggcccgga catcctcgtc aacaccgccg gcatcgccga
     1441 gagcgcgccg ctcgtcagga cggacgacga gctgtggcag cgcctcatcg gcgtcaacct
     1501 ctcgggcgtc ttctactgca cgcgcgcggc gctgcccacg atgctcgggc gcggctgggg
     1561 ccgcgtcatc aacgtcgctt cggtcgcggc gaagacgggc gcgccctaca tctcggccta
     1621 cacggcgtcg aagcacggcg tgctcggcct gacgcgggcc gtcgcgctcg aagtcgcgga
     1681 gaagggcgtc acggtcaacg ccatctgccc tggctacgtc gagacggaca tgacgtgggg
     1741 cgccgtcgag aacatcaccg cgcgcacggg c
//
LOCUS       DBNGOH010000772         1805 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3781757, whole genome shotgun sequence.
ACCESSION   DBNGOH010000772 DBNGOH010000000
VERSION     DBNGOH010000772.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1805)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1805)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1805
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3781757"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            183..1697
                     /locus_tag="AC159P_14860"
     CDS             183..1697
                     /locus_tag="AC159P_14860"
                     /inference="COORDINATES: protein motif:HMM:NF017044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HIR3696780.1"
                     /translation="MKRTLLQRRLSVFALALATLAAPGLCIVRTHAAPPQKAGAATRQ
                     AKPLPKPSPQAGRPASKPLAEPAPLVNKVLANGLEIIVLEDHSVPLVTVEMAVRNGSF
                     TETPELNGLSHLYEHMFFKANRALANGEEYMKTIDHLGIISNGETREEIVNYYFTTTS
                     PNFPVAMRFMRDAARYPLFHPGQFETEREAVIAELDRHESNPYGAIGQEMNKRLFYKY
                     PTRKSPGGTKETVRSATTDMMRLIQSRYYVPNNSAIVVTGDVTPAEVFRHVEDLFGDW
                     ERAEDPFKKFPLVEHPALPKSEGAVLTSPIQNVVIMVGWHGPSIGKDDAATYAADVFS
                     FILRQPGSRFQRALVDTGLVTGVGISYYTQRNVGPIQIIAQTTPDKARAALKAIYEEV
                     AHFNDRDYFTDEQLESAKTLLEADDLYQREKLSEYAHTISFWWSSTGLDYFRGYLPRL
                     RATSRADNSRYVTDYIQGKPHVGLALMSEAAAKESGVTPDDLIGAGTRNSTAGR"
BASE COUNT          337 a          654 c          531 g          283 t
ORIGIN      
        1 tactgcctac tgcctactgc ctactatttc tgcctttctt cgagagcaac ccggcgtggt
       61 atcatcgagt caaagatgtg aatcgaagtt cgggtcggta tccgcggtaa gtcccctgtt
      121 gctcctgccg acgccccgcc cgcgcgggcg attcccgagt ggtgacaaac gaagaaggtt
      181 ttatgaagcg aactctcttg caacgccgcc tttctgtctt cgcgctcgcg ctcgccaccc
      241 tggccgcgcc gggcctctgc atcgtccgga cgcacgccgc gccgccgcag aaggcgggcg
      301 cggcgacgcg gcaggcgaag cctctgccga agccctcgcc gcaggccggg aggcccgcct
      361 cgaagccgct ggccgagccg gcgccgctcg tcaacaaggt cttggcgaac gggcttgaga
      421 tcatcgtgct cgaagaccat tcggtgccgc tcgtgacggt cgagatggcc gtgcgcaacg
      481 gctcgttcac cgagacgccg gagctgaacg gcctgtcgca cctctacgag cacatgttct
      541 tcaaggccaa ccgcgcgctc gccaacggcg aggagtacat gaagaccatc gaccacctcg
      601 gcatcatctc gaatggcgag acgcgcgagg agatcgtcaa ctactacttc accacgacca
      661 gcccgaactt ccccgtcgcg atgcgcttca tgcgcgacgc cgcgcgctac ccgctcttcc
      721 accccggcca gttcgagacc gagcgcgagg ccgtcatcgc cgaactcgac cgccacgagt
      781 cgaaccccta cggcgccatc ggtcaggaga tgaacaaacg cctcttctac aagtacccga
      841 cgcgcaagag tccgggcggc acgaaggaga ccgtgcgctc ggcgacgacc gacatgatgc
      901 gcctcatcca gagccgctac tacgtgccga acaactcggc catcgtcgtc accggcgacg
      961 tgacgcccgc cgaggtcttc cgccacgtcg aagacctgtt cggcgactgg gagcgcgccg
     1021 aagacccgtt caagaagttc ccgttggtcg agcacccggc gctccccaag agcgagggcg
     1081 cggtcttgac cagccccatc cagaacgtcg tcatcatggt cggctggcac ggcccctcca
     1141 tcggcaagga cgacgcggcg acctacgcgg cggacgtctt ctcgttcatc ctccgccagc
     1201 ccggctcgcg cttccagcgc gcgctcgtcg atacgggcct cgtcaccggc gtcggcatca
     1261 gctactacac gcagcgcaac gtcggcccca tccagatcat cgcgcagacc actcccgaca
     1321 aggcgcgcgc cgccttgaag gccatctacg aagaggtcgc gcacttcaac gaccgcgact
     1381 acttcaccga cgagcagctc gaaagcgcca agacactcct cgaagccgac gacctctacc
     1441 agcgcgagaa gctctcggag tacgcgcaca cgatcagctt ctggtggtct tcgaccggcc
     1501 tcgactattt ccgcggctac ctgccgcgcc tgcgcgccac ctcgcgcgca gacaacagcc
     1561 gctacgtcac cgactacatc cagggcaagc cgcacgtcgg cctcgcgctg atgagcgagg
     1621 ccgcggcgaa ggagtcgggc gtgacgcccg acgatttgat cggcgccggc acgcgcaaca
     1681 gcaccgcggg caggtgaagg cgcgaccgca cgccgcacgc tcgtcccggc cttgaaaggc
     1741 cgagctaaat tcaaacgccg ctacgcggcg cgttgcgggt tcgggttatc tttcggaatt
     1801 tatcg
//
LOCUS       DBNGOH010000773         3453 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3415862, whole genome shotgun sequence.
ACCESSION   DBNGOH010000773 DBNGOH010000000
VERSION     DBNGOH010000773.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3453)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3453)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3453
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3415862"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..338)
                     /locus_tag="AC159P_14865"
     CDS             complement(<1..338)
                     /locus_tag="AC159P_14865"
                     /inference="COORDINATES: protein motif:HMM:NF019265.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type sigma factor"
                     /protein_id="HIR3696781.1"
                     /translation="MTRSSTHDVTELLLAWGNGDEAALEQLAPLIHAELHRLAHLYMS
                     RERPDHTLQTSALINEAYVRLIDWKAARFQNRAHFFGVAAQLMRRILVDVARSRPKAE
                     GGAPAQHIAL"
     gene            628..1167
                     /locus_tag="AC159P_14870"
     CDS             628..1167
                     /locus_tag="AC159P_14870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696782.1"
                     /translation="MKAMRSITRLTATALVAASLFQLPTRAHPTVINAGAARSSHVLY
                     QDIFIPAGLAPGQTLRCTWANLGDLDPLKRELEPLRIEVKLLTGEGSVIAQAGAAAVG
                     AGRFQSFDFDRDALPPAGEPGTGRLQVQVRVEVSFHATLPLPDAPKLFPASLELVDNV
                     TGQTLRSFALLDRSRAVGF"
     gene            1279..1824
                     /locus_tag="AC159P_14875"
     CDS             1279..1824
                     /locus_tag="AC159P_14875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696783.1"
                     /translation="MKIGHYFLLLLALFTGAAPAQAAAPTRPQTTEGHQCLVFFLGGM
                     PGLTRGQTLRYTWANLGDPDPLKREFEPLRIRATLFAADGSVIAQEEAAAVGAGRFQS
                     FDFVRDRINLPGDAGTGRLQVRLEAVVIGRLTNPDLTLDREILESFDAAVEVIDTLTG
                     RTTVSLKPKEIVVVASKPPSQ"
     gene            1897..2143
                     /locus_tag="AC159P_14880"
                     /pseudo
     CDS             1897..2143
                     /locus_tag="AC159P_14880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247312.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
     gene            2587..3135
                     /locus_tag="AC159P_14885"
     CDS             2587..3135
                     /locus_tag="AC159P_14885"
                     /inference="COORDINATES: protein motif:HMM:NF019325.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CARDB domain-containing protein"
                     /protein_id="HIR3696784.1"
                     /translation="MNRMKLIFAALLVASAAAPAFSQGEAKRSAPRSTRRDAATFALV
                     ATRFKNAGGNYSQRQSQFPAPTDRLKARPGQPDLLVEQFVFAGEKALRVRVANVGSAP
                     SGPCVLRLTVRKINGVAVGRTTEVKLLPIAAGKDRWVAINAKSILPNNIALDSTTFRL
                     NADATAVVAETNEDNNEVWHNL"
BASE COUNT          610 a         1150 c         1106 g          587 t
ORIGIN      
        1 tcgagcgcga tgtgttgcgc gggggcgccg ccctctgcct tcgggcggct gcgcgcgaca
       61 tccaccagga tgcggcgcat caactgcgcg gccacgccga agaagtgcgc gcggttctgg
      121 aagcgcgcgg ccttccagtc gatcaggcgc acgtaggctt cgttgatgag cgcggaggtc
      181 tggagcgtgt ggtcggggcg ctcgcggctc atgtagaggt gggccaggcg gtgcagctcg
      241 gcgtgaatga gcggggcaag ctgttcgagc gccgcttcgt cgccgtttcc ccaggcgaga
      301 agcaactccg tcacgtcgtg tgtcgaagaa cgtgtcatgg cagccgtcct cccagcttgt
      361 gcggggcaat ttatcacacg cgaggcgccc gctcatacct cattccgacg ggccgcgtca
      421 ccgccgaagc gcgctgacgc ctcggcccgt cggtagcaat atacggcgcg cacatgccgc
      481 cccggttttc tttccccgca ccgcgccttc gccccgctcc ccgaaaaatt ctgcgcgccc
      541 gaggacagat ttcgcgcacg cctttcgcat tacctagtga ggcggctttg aagcgggcga
      601 tgttcagccc ggaaggagtt cgaagggatg aaagcgatga ggagtatcac acggttgact
      661 gcaacggctc tggtcgccgc cagtttgttc cagcttccaa cccgcgcaca tccgaccgtg
      721 atcaacgccg gcgcggctcg atcgtcccac gtgctgtacc aagacatctt tatccccgcc
      781 gggttggcgc cggggcaaac cttgcgttgc acgtgggcga atctcggcga cctcgaccca
      841 ctgaagcgcg agcttgagcc gttgcgcatc gaggtcaagc tgctcaccgg cgaggggagc
      901 gtgatcgcgc aggccggggc ggcggcggtc ggcgcgggcc gatttcaatc cttcgacttc
      961 gatcgcgatg ctctgccgcc ggcgggcgaa cccggcacgg ggcggttgca ggtgcaagta
     1021 cgagtagagg tcagcttcca cgccacgctt cccttgcctg acgcgccgaa gcttttccct
     1081 gcctcgctcg aactcgtgga taacgtcacc ggccagacgt tgcgctcctt cgcactcctg
     1141 gatcgttccc gcgccgtcgg gttctagcgg gtatcgacgg cgaccggacg gcgggaccgc
     1201 cgcttccggc gtttcggacg ttgcgatgtc cttgtcggcg agccgatgaa ggttccgatt
     1261 aagtgaacgg agaggcagat gaaaatcggt cactacttct tgttgctgtt ggccctgttt
     1321 accggcgcag ccccggcgca agcagccgcg cccacgcgcc cgcaaaccac ggaaggccat
     1381 caatgcctcg tctttttcct gggcgggatg cccggactca cgcgcgggca gacgctgcgt
     1441 tacacctggg cgaacctcgg cgaccccgac ccgctgaagc gcgagttcga accactgcgc
     1501 atccgagcca cgctgttcgc cgccgacggg agcgtgatcg cgcaggagga agcggcggcg
     1561 gtcggcgcgg gccgatttca atccttcgat ttcgtccgcg accggatcaa cttgccgggc
     1621 gatgcgggca ccggtcgtct gcaagttcga ctggaggcgg tagtgatcgg acgtctcact
     1681 aatcccgatc tcacgctcga ccgtgaaatc ctcgaatcct tcgacgccgc ggtggaggtc
     1741 attgacacgc tcaccgggcg caccaccgtc agcctcaaac cgaaggagat tgtcgtcgtc
     1801 gcctcgaagc cgcccagtca atagaccgcg ccgcgccccg tgcgtgcccg accaggcatg
     1861 gaagttgaac cataactttt cgagaggagg cttctcatga tcatccgacc gcatacgtcg
     1921 ctcgcaccgg ccagtcatcc cgtccacgac gcgaaagagc gtccgatctc gcggtttgat
     1981 ccgaggcgcc aggcggggtt caccctgatc gagctgctcg tcgtgatcgc gatcatcgcg
     2041 gtccttatcg ggatgctcct gcccgccgtc caggccgcgc gggaggcggc gaacaaggcg
     2101 cgttgcacga acaacctcaa acagctcggg cttgcccaga tcgattattt ccgggcgcac
     2161 cgtttgtacg cctcctcgtt cgaggaactc gggctgtcgc agcccgcgga cggggaaagg
     2221 ggcgcgggcc gtcaagtact caacggcgtc gtcatcgcac agacggggat cggtcgcttc
     2281 gccgggacgc ccggcaccgg gcgcgtgacg tttgactgga gggacggttt cgacgggccg
     2341 ttcgatgcga cgttcaacgt gaacccgttt gcgaagcgcc gcagagagta agtgtgtggc
     2401 aggggcatcg gcctgtcaca cttcgaccag gcggcgggac tcgaacctcc gggttggacg
     2461 gtgcatggtc ggcttctcta acgacgtggc ctgaaaaatt ccgcacaccg gatgccagtt
     2521 atcacgcacc cttttcgcat taacaagtga gggcagccag agacgctgcc gccaaaggag
     2581 aacatgatga accgtatgaa actgatcttc gccgccctgc tcgtcgcttc cgccgccgcg
     2641 cccgccttct cgcaaggcga ggcgaaacgc agcgcgccgc gcagcacacg acgcgacgcc
     2701 gcgaccttcg ccctcgtcgc aaccagattc aagaacgcgg ggggcaacta ctcgcagagg
     2761 cagtcgcagt tccccgcccc gaccgacagg ctcaaggccc gaccgggcca acccgacctg
     2821 ctcgtcgagc agttcgtctt cgcgggcgag aaggctttgc gcgtccgcgt cgccaacgtc
     2881 ggcagcgcgc cctccggccc ctgcgtcctg cgcctgaccg tccgcaagat caacggcgtc
     2941 gccgtgggcc gcacgaccga ggtcaagctc ctgccgatag cggcgggcaa agacaggtgg
     3001 gtcgcgatca acgccaagag catcctgccg aacaacatcg cgctcgactc cacgaccttc
     3061 aggctcaacg cggacgccac cgccgtcgtc gccgagacga acgaggataa caacgaagtc
     3121 tggcacaacc tgtaatgcag gggccgacca gaggcgaacc gagcgtcgcc gctcggttcg
     3181 ccgggtcacg gcgaaagggg ctgccgcaga gtcgatggtc gaagagggag acggcagccg
     3241 ctttccctcc gtcgaaaaat aaatccgcgg cggctgctag ttttcgcgtc cgccctgcgc
     3301 attaacgaag tgagggccgc cgggtgcctc cccgagaagc atagaaatga ggtctcccat
     3361 gggcaacaaa cgcaacacgc cggccgttct ggccgtagcc ctgatcttcg tctgcttgag
     3421 cctacgggcc ggcgcgggcg cgacacgcgc ggc
//
LOCUS       DBNGOH010000774         2998 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3598201, whole genome shotgun sequence.
ACCESSION   DBNGOH010000774 DBNGOH010000000
VERSION     DBNGOH010000774.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2998)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2998)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2998
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3598201"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2198
                     /locus_tag="AC159P_14890"
     CDS             <1..2198
                     /locus_tag="AC159P_14890"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018476780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HIR3696785.1"
                     /translation="ESNPHFTLDQKRVYFTRQNNLYVMSLDGGSLEQLTDIRVPPPAG
                     AAGAQLAQGAGRGAGGENVEVLRKEERALLEAVRERTEFREEQEKKRRERERRRPLNL
                     TQGQTVAALSLSPDGARVVVAVNEQAPGARNTIVPNYVTDTAYTEDINSRTKVGDQQG
                     RSRMGLVSTETGEVKWVEHGQRVEVAPTVRPRTEANATEAAQREQGVQTGTTQQAQQQ
                     TQQQSGQTQSQQQPPPTQAQQQQTEGAQTQQARDRDVQLFQLQWSEDGARAVLMARAA
                     DNKDRWVLALDAATGKTRVLARVHDDAWVGGPGSFTLGWLADNRRVYFVSERDGFAHL
                     YTVNADGGEPTQLTSGRFEVSDVRLSRDKTKFYFTSSEGSFFERHLYTMPVEGGARTR
                     LTTMPGNNQSDISPDEQLLAVVNSYSNRPPELFIQANRPAPPTDAKQPMPVRVTSSPT
                     EEWLSHNWIDPPVVSFKARDGATVHARLYKPANFQRGGPAVLFVHGAGYLQNAHKWWS
                     TYYREYMFHHLLMERGYAVLDIDYRGSAGYGRDWRTGIYRHMGGKDLTDHVDGVRYLV
                     AEHGVDPKRVGLYGGSYGGFITLMAMFTEPEVFAAGAALRPVTDWAHYNHPYTANILN
                     TPQGDLEAYRRSSPIYFAEGLKGALLICHGMVDVNVHFQDTVRLTQRLIELRKENWEL
                     AAYPVEDHSFERASSWADEYKRILKLFTEHLHPDAGLSSGGNNGNARKR"
     gene            2237..2788
                     /locus_tag="AC159P_14895"
     CDS             2237..2788
                     /locus_tag="AC159P_14895"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3696786.1"
                     /translation="MFSYKIDGQTELRLLEERHAEKVFSLLEQNRSHLEAELTWLDDQ
                     FSLKDAKEYIRAGLERFAANNGLWAGIWLRDDLAGIVSLHNLVRADRKASLGYWLGAS
                     FQGRGLVTKACRILVDHAFGELKLNRLEIQCDPENVRSRKVAGRLGFTQEGVLRQSWW
                     SRDRFVDQMVYGLLASEWQGAQP"
BASE COUNT          567 a         1023 c          950 g          458 t
ORIGIN      
        1 ccgagtcgaa cccgcatttt acgttggacc agaagcgcgt ctatttcacg cgccagaata
       61 atctctacgt gatgtcgctc gacggcgggt cgctcgaaca gttgacggac atccgcgtgc
      121 cgccgcccgc gggtgcggcg ggcgcgcagc tcgcgcaggg cgcggggcgc ggcgccgggg
      181 gcgagaacgt ggaggtgttg cgcaaagagg agcgcgcgct gctcgaagcc gtgcgcgagc
      241 ggacggagtt ccgcgaggag caggagaaga agcggcgcga gcgcgagcgg cgccggcccc
      301 tgaaccttac gcaggggcag acggtggccg cgctctcgct gtcgccggac ggcgcgcgcg
      361 tcgtcgtcgc ggtgaacgag caggcgcccg gtgcgcggaa caccatcgtc cccaactacg
      421 tcacggacac ggcgtacacc gaagacatca acagccgcac gaaggtcggc gaccagcagg
      481 gccgctcgcg gatgggtctc gtctcgaccg agacgggcga ggtcaagtgg gtcgagcacg
      541 ggcagcgcgt cgaagtcgcc cccaccgtgc gcccgcgcac cgaagcgaac gcgaccgaag
      601 ccgcgcagcg cgagcagggc gtccagaccg gcaccacgca gcaggcgcag cagcagacgc
      661 agcaacagtc cgggcagacg cagagccagc aacagccgcc gcccacgcag gcgcagcagc
      721 agcagaccga aggcgcgcag acgcagcagg cgcgcgaccg cgacgtgcag ctcttccagc
      781 ttcagtggtc ggaggacggc gcgcgcgccg tcctgatggc gcgcgccgcg gacaacaaag
      841 accgctgggt gttggcgctc gacgcggcga ccggcaagac gcgcgtgctc gcgcgcgtcc
      901 acgacgacgc gtgggtcggc ggccccggct cgttcacgct cggctggctc gcggacaacc
      961 gccgcgtcta cttcgtctcc gagcgcgacg gcttcgcgca cctctacacg gtgaacgcgg
     1021 acggcggcga gccgacgcag ctcacctcgg ggcggttcga agtctcggac gtgcggctct
     1081 cgcgggacaa gacgaagttc tacttcactt cgagcgaggg cagcttcttc gagcgccacc
     1141 tctacacgat gccggtcgaa ggcggcgcgc gcacgcgcct caccacgatg cccggcaaca
     1201 accagtcgga catctcgccc gacgaacaac tgctcgccgt cgtcaactcc tacagcaacc
     1261 gcccgcccga gctgttcata caggcgaacc gccccgcgcc gcccacggac gccaaacagc
     1321 ccatgcccgt ccgcgtgacc agctcgccca cggaggagtg gctctcgcac aactggatcg
     1381 acccgcccgt cgtcagcttc aaggcgcgcg acggcgcgac cgtccacgcg cgtctctaca
     1441 agcccgccaa cttccagcgc ggcggccccg ccgtcctctt cgtccacggc gccggctact
     1501 tgcagaacgc gcacaagtgg tggagcacct actaccgcga atacatgttc caccaccttt
     1561 tgatggagcg cggctacgcc gtcctcgaca tcgactaccg cggcagcgca ggctacgggc
     1621 gcgactggcg cacgggcatc taccgccaca tgggcggcaa ggatttgacc gaccacgtgg
     1681 acggcgtccg ctacctcgtc gccgagcacg gcgtcgatcc caagcgcgtc ggcctctacg
     1741 gcggctccta cggcggcttc atcacgctga tggcgatgtt caccgaaccc gaagtgttcg
     1801 ccgccggggc ggcgctccgc cccgtcaccg actgggcgca ctacaaccac ccctacaccg
     1861 ccaacatcct caacaccccg cagggcgacc tcgaagccta ccgccgcagc tcgcccatct
     1921 acttcgccga aggactcaag ggcgcgctcc tcatctgcca cggcatggtc gatgtcaacg
     1981 tccactttca agacacggtg cgcctcacgc agcgcctcat cgagctgcgc aaagagaact
     2041 gggaactcgc cgcctacccc gtcgaagacc actccttcga gcgcgcgtct tcctgggccg
     2101 acgagtacaa gcgcatcctc aaactcttta cagaacacct acaccccgac gccggcttat
     2161 catccggcgg aaacaacggc aatgcgcgca agcgctagat gaccagaccg caacggcttc
     2221 gcggggttat cgaatcatgt tcagctacaa aattgacggc cagacagagc ttcgcttgct
     2281 tgaagagcgg catgcggaaa aagtcttttc cttactggag cagaaccgaa gccatttaga
     2341 ggcggaactc acgtggcttg atgaccagtt ctctcttaag gacgccaagg aatacattcg
     2401 agccgggctt gagcggttcg cggccaacaa cggcctgtgg gcaggcatct ggctgcgaga
     2461 cgacctggcc ggaatagtca gtctgcataa cctcgtccgg gctgatcgga aggcgagtct
     2521 cggttactgg ttgggcgcct cttttcaagg ccgggggctg gtgacgaagg cttgccgtat
     2581 tttagttgac cacgccttcg gcgagctgaa gctgaatcgt ctggagatac aatgcgaccc
     2641 cgaaaatgtt cggagccgga aggtcgccgg gcgattgggt ttcacacagg aaggtgtgtt
     2701 gcgtcagtcc tggtggagta gagaccgttt cgtggatcag atggtttatg gcttgcttgc
     2761 ctccgaatgg caaggcgcgc aaccttgaat tcgaatacca tgcgaggaga tggatgttat
     2821 tgcctgtcat tcaactcgac cccgcgacgg caagcccctc atcctcgtct tctcggcaac
     2881 gccgaaggcc gctcgcgcgg ggcgggtgat gccggggtgg cggcccatga tcgggtgtgc
     2941 ccggacaccc gctgctcccg gttcggctcc tttatttctc tccggacagt aagcgcgt
//
LOCUS       DBNGOH010000775         3400 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1399995, whole genome shotgun sequence.
ACCESSION   DBNGOH010000775 DBNGOH010000000
VERSION     DBNGOH010000775.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3400)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3400)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3400
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1399995"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1152
                     /locus_tag="AC159P_14900"
     CDS             <1..1152
                     /locus_tag="AC159P_14900"
                     /inference="COORDINATES: protein motif:HMM:NF013126.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HIR3696787.1"
                     /translation="VTQLDRHWRELLREVRPGGVILFGRNVESAEQVALLNAQVRDAC
                     GGRVLVGVDQEGGLVDRFRQVCEPSPSAKAVRYARSAALADKFGWLTARLLRLTGFNI
                     NFAPVLDLTGDNEENGLRARTFGETPELVTLLAGAYLDGLQRGNVTGCGKHFPGLGGS
                     TVDSHGGLPVITHTWEEIFERDLVPFMDLMFHRPGERLKSVMVSHAAFPGVSEFLQAW
                     FRRTGDPPTIEGLHRFPATISGNVLMRLLRVILKYDGLVITDDMEMGAVVQTLSVAEA
                     SLRAIEAGSDMVLICEHESNFVAARDRLAEAMREERLSVRAVNAALRRMDRALDMAGE
                     PEQFDAAEFLAVSQRIGELNRELKAASESGEYAPLYGTGEGGTRHSSNF"
     gene            1441..2895
                     /locus_tag="AC159P_14905"
     CDS             1441..2895
                     /locus_tag="AC159P_14905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002631058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HIR3696788.1"
                     /translation="MRRTLSFAVALALVFVHSTAQQQQPAPQRDPATPPATAALPPPQ
                     GPLPAPRLRAETYADEAARIIGAALTDDTAYRRLAYLGDRIGHRLSGSAQLERAIEWA
                     VAEMKKDRLDNVRTEMVMVPHWVRGAESLELLEPASTTRELAMLGLGGSVGTAPEGLT
                     AEAVVVRTFDELDALGERVRGKIVLYNAPFTTYGATVQYRGSGPSRAARYGAVAALVR
                     SVGPVSLQTPHTGALRYDEAQPRIPAAAVTIEAAEMMQRMQERGETIRLRLKMEAKFL
                     PDAESANVVAELKGRERPEEIVLVGGHFDSWDVGQGAHDDGGGCMVAWETVRLLKKLN
                     LRPRRTIRAVLFTNEENGLRGANAYRDAHRTEIANHLLAIESDSGVFRPTGFGLAQAA
                     PPQARADLREIARLLRGLRADRIDADGGGADIGPLMREGVIGASLDVDGPYFNYHHTR
                     ADTVDKVDPPELAACVAAMAVMAYVVADMPPRLR"
     gene            3145..>3400
                     /locus_tag="AC159P_14910"
     CDS             3145..>3400
                     /locus_tag="AC159P_14910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696789.1"
                     /translation="MENSGLGYTEFDIRRVIPGEAEAVRVRIAAVLEGFNYRVLNEQP
                     LQARRSQQKNILAANSLDLSTKLTVGLKNLSQHATLATFDY"
BASE COUNT          570 a         1207 c         1127 g          496 t
ORIGIN      
        1 gtgacgcagc ttgaccgcca ctggcgcgag ctgctgcgcg aggtgcggcc cggcggcgtg
       61 attctcttcg ggcgcaacgt cgagtcggcg gagcaggtgg cgctcctcaa cgcgcaggtg
      121 cgcgacgcgt gcggcgggcg cgtgctggtc ggcgtcgatc aggagggcgg gctggttgat
      181 cgcttccggc aggtgtgcga gccgtcgcct tcggcgaagg ccgtgcgcta tgcgcgcagc
      241 gccgcgctcg cggacaagtt cggctggctc accgcgcggc tgctgcgcct gacgggcttc
      301 aacatcaact tcgcgcccgt gctcgacctc acgggcgaca acgaggagaa cggcctgcgc
      361 gcgcgcacct tcggcgagac gcccgagctg gtgacgctgc tggcgggcgc ctacctcgac
      421 ggcttgcagc gcggcaacgt gacgggctgc ggcaaacact tcccgggcct cggcggctcg
      481 accgtcgatt cgcacggcgg cctgcccgtc atcacgcaca cgtgggagga gattttcgag
      541 cgcgacctcg tgcccttcat ggacttgatg ttccaccgac cgggcgagcg cctgaagtcg
      601 gtgatggtga gccacgcggc gttccccggc gtgtccgaat tcttgcaggc gtggttccgc
      661 cgcaccggcg acccgccgac catcgagggg ctgcaccgct tccccgcgac catcagcggc
      721 aacgtcctca tgcgcctctt gcgcgtcatc ctcaagtacg acggcctcgt catcacggac
      781 gacatggaga tgggcgccgt cgtgcaaact ctgtcggtcg ccgaagccag cttgcgcgcc
      841 atcgaggccg gctccgacat ggtgttgatc tgcgagcacg agtcgaactt cgtcgcggcg
      901 cgcgaccgac tggccgaggc catgcgcgaa gagcgtttga gcgtgcgcgc cgtcaacgcc
      961 gcgctccggc gcatggatcg cgcgctcgac atggcgggcg agccggagca gttcgacgcc
     1021 gcggagttcc ttgcggtctc gcaacgcatc ggcgaactca accgcgagct gaaggccgcc
     1081 tcggagtcgg gcgagtacgc gccgctctac ggcacgggag aaggcggcac gcgccattcg
     1141 tcgaacttct gatggtcgaa tcatcaatct gccggagctg ttttgcggtt gagtgtcggg
     1201 cgggttcgtt tcaacacaga gggcacggag aaggcgcaga ggacacagag aagaagattg
     1261 tctttctctg tgccctctgt ggaatctctg tgaactctgt gttgaaacga accctcggta
     1321 aactcaacga cgaagcgctc cgaatgctcg gcggcatcgt tcccgacagc atcactctca
     1381 acgagcatcg aaccccgcgc ggtgccccga ccgaacagca caggaggctt cagaaccgtc
     1441 ttgcgtcgca cactctcgtt cgccgtcgcc ctcgcgctcg tcttcgtcca ctcaaccgcg
     1501 caacagcagc agcccgcccc gcagagagac ccggccacgc cgcccgcgac cgccgcgctg
     1561 ccgccgccgc aaggcccgct gcccgcgccg cgcctgcgcg ccgaaacgta cgccgacgag
     1621 gccgcgcgca tcatcggcgc ggcgctcacg gacgacacgg cctaccgccg gctcgcctac
     1681 ctcggcgacc gcatcggcca ccgcctcagc ggctccgcgc agcttgagcg cgccatcgag
     1741 tgggccgtcg ccgagatgaa gaaagacagg ctcgacaacg tccgcaccga gatggtgatg
     1801 gtgccgcact gggtgcgcgg cgcggagagc ctcgaactgc tggaaccggc gagcacgacg
     1861 cgcgagctgg cgatgctcgg cctcggaggc agcgtcggca ccgcgcccga ggggctgacg
     1921 gcggaggccg tcgtcgtccg caccttcgac gagctggacg cgctcggcga gagagtccgc
     1981 ggcaagatcg tcctctacaa cgcgcccttc accacttacg gcgcgaccgt gcagtaccgc
     2041 ggctcaggcc cctcgcgcgc ggcgcgctac ggcgcggtcg cggcgctcgt ccgctcggtc
     2101 ggccccgtca gcctccagac gccgcacacc ggcgcgctcc gctacgacga ggcgcagccg
     2161 cgcatccctg ccgccgccgt caccatcgag gccgccgaga tgatgcagcg catgcaggag
     2221 cgcggcgaga cgatccgcct gcgcctcaag atggaggcta agttcctgcc cgacgccgag
     2281 tcggccaacg tcgtcgccga gttgaaaggc cgcgagcggc ccgaggagat cgtcctcgtc
     2341 ggcggccact tcgactcgtg ggacgtgggg cagggcgcgc acgacgacgg cggcggctgc
     2401 atggtcgcgt gggagaccgt aaggctgctc aagaaactga acctccgccc gcgccgcacc
     2461 atccgcgccg tgctcttcac caacgaggag aacggcctgc gcggcgccaa cgcctaccgc
     2521 gacgcgcaca ggacggagat cgccaaccac ctgctcgcca tcgagtccga ctccggcgtc
     2581 ttccgcccca ccggcttcgg cctcgcgcag gcggccccgc cgcaggcgcg cgccgacctg
     2641 cgcgagatcg cccgcctgct ccgagggcta cgcgccgacc gcatcgacgc cgacggcggc
     2701 ggcgcggaca tcggcccgct catgcgcgag ggcgtcatcg gcgccagcct cgacgtggac
     2761 ggcccctact tcaactacca ccacacgcgc gccgacaccg tcgataaagt tgacccgccg
     2821 gaactcgccg cctgcgtcgc cgcgatggcc gtcatggcct acgtcgtcgc cgacatgccc
     2881 ccgcgcctgc ggtgagcagt aggcagcggg cagcaggcag tgaaagaagc cgggttaaac
     2941 tattggcctt gagagttgag gtttgaaagt cgcgatttca ggcatcgact ctgaagctca
     3001 gaactcgaaa ccactcttgt attaactgcc agctgcctac tgcctactgc ctactgctcg
     3061 ctaaacatcg ggcgggcgtc cgacgtaata acaacagtcg gacgcacatc gggggccttg
     3121 cacgcggcgc ggaggcaatt cgtgatggag aacagcggac tcgggtacac ggagttcgac
     3181 atcaggcggg tcatccccgg cgaggcggag gccgtgcgcg tgcgcatcgc cgccgtgctc
     3241 gaaggcttca actaccgcgt gctgaacgag cagccgcttc aggcgcggcg gagccagcag
     3301 aagaacatcc tcgcggccaa cagcctcgac ctatccacga agctcacggt cggcctcaag
     3361 aacctgagcc agcacgcgac gctcgcgacg ttcgactacg
//
LOCUS       DBNGOH010000776         3088 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_5065878, whole genome shotgun sequence.
ACCESSION   DBNGOH010000776 DBNGOH010000000
VERSION     DBNGOH010000776.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3088)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3088)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3088
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_5065878"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2698
                     /locus_tag="AC159P_14915"
     CDS             <1..2698
                     /locus_tag="AC159P_14915"
                     /inference="COORDINATES: protein motif:HMM:NF012264.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR3696790.1"
                     /translation="GHGRHKYWLHPLFEKENMEAGCNQCHTSDRVLQGAPNLTLGKDL
                     FSERGCVGCHRYEGFDRETDALTNTRQLIKQYDDEITANERAAKAARAESAATTSDEV
                     AQQLLARSEGLVVTNSQIEARRDELETQARYLMQDQKKVGPNLKDIRLKLRKEWLPVW
                     LKDPQAFRPGTKMPTYWYLSGDEKMAKGNIVPASRQEEERKAIAAYLWQSAFEGAVPT
                     QPRGDGARGKELFEVTGCMACHSLGEGEQKVGGDFAANLQRLGEKANYDYIVRWVHNP
                     RERWAPYCPKHKRDLTPEDYAKHNKPYVFDARNSRCPEDGAELQVQNMTVMPNFRLTD
                     QDARDIATYLVSLTSGASYPDASFMDDPKLKDTGRDLIKQYGCAGCHEIRGFEEEQRI
                     GKELTAEGATPLERLDFAMLTHKAELGIDPFTDKKTATRTRDTTKPGWYDHKGFFDRK
                     LEDPSVYDMGKEKEPRDHLRMPKPYLKPEWKVALTTFLLGSVGNEGANIPTSLFYQPN
                     EQQKAIQDGWWVIKKYNCMGCHNVQVGQQSDLSTLPFYQEGGALGKEQLPPGLTTEGA
                     RVDPDWLLRFLTDPSLSGVSLDARSLARPGQISGANPALAARGQQPANGTPVGNPPGA
                     QPNAAAQPNAISNAHAALKPQPGAPRNGVRTYLRARMPTFNFSPNELRTLVRFFQAVS
                     AQHEPFIKEQAEPLTDAERDLARALFTSQAAPCLKCHVTSEGEQGKTAPNFLIAGERL
                     KSDWTYRWLLDPQRIMPGTAMPSELFKREGERWVFNGPLPPSAADYQGDHARLLVRYL
                     LQLTPEEQARSARSAASAPAVTPAAPAAAQPATPVASHRRAKTPRAAAARPTENAGRR
                     VRRARRQQASMRPAPARRARRGSRGQAAAATARPRRAPDFSR"
     gene            2750..>3088
                     /locus_tag="AC159P_14920"
     CDS             2750..>3088
                     /locus_tag="AC159P_14920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696791.1"
                     /translation="MQSKRARFLAALIAALCLLAFTAACGRDEDAEDPEGPEGQTEET
                     DVVLTPFKPTGNEGAIAGTVSFAGTPPTPPDISMLADAFCASANQNPKAEDTVVNNGT
                     LQNVFVYVKDG"
BASE COUNT          581 a         1167 c          972 g          368 t
ORIGIN      
        1 gggccacggc aggcacaaat actggctcca cccgctcttc gagaaggaga acatggaggc
       61 cggctgcaac cagtgccaca cgagcgaccg cgtcctgcaa ggcgccccca acctcacact
      121 cggcaaagac ctcttcagcg agcgcggctg cgtcggctgc caccgctacg agggcttcga
      181 ccgcgagacg gacgcgctga cgaacacgcg ccagctcatc aagcagtacg acgacgagat
      241 caccgccaac gagcgcgccg cgaaggccgc gcgcgccgag agcgccgcca cgacgagcga
      301 cgaggtggcg cagcagcttc tggcgcgctc cgagggtctg gtcgtcacca acagccagat
      361 cgaagcgcgg cgcgacgagc ttgagacgca ggcgcgctac ctcatgcagg atcagaagaa
      421 ggtcggcccg aacctcaagg acatccgcct caagctccgc aaggagtggc tgccggtctg
      481 gctcaaagac ccgcaggcgt tccgccccgg cacgaagatg ccgacctact ggtatctctc
      541 gggcgacgag aagatggcga agggcaacat cgtccccgcg agccggcagg aggaggagcg
      601 caaggccatc gccgcctacc tctggcagtc cgccttcgag ggcgcggtgc ccacgcagcc
      661 gcgcggcgac ggcgcgcggg gcaaggagtt gttcgaggtg acgggctgca tggcctgcca
      721 ctcgctgggc gagggcgagc agaaggtcgg cggcgacttc gcggccaacc tccagcggct
      781 cggcgagaag gccaactacg actacatcgt ccgctgggtg cacaacccgc gcgagcgctg
      841 ggcgccctac tgcccgaagc acaagcgcga cctcacgccc gaagactacg ccaagcacaa
      901 caagccctac gtcttcgacg cgcgcaactc gcgctgccca gaggacgggg ccgagcttca
      961 ggtgcagaac atgacggtga tgcccaactt ccgcctcacc gatcaggacg cgcgcgacat
     1021 cgccacctac ctcgtctcgc tcacgtccgg cgcctcgtac ccggacgcct ccttcatgga
     1081 cgacccgaag ctgaaggaca cgggccgcga cctcatcaag cagtacggct gcgccgggtg
     1141 ccacgagata cgcggcttcg aggaggagca gcgcatcggc aaggagttga ccgccgaggg
     1201 cgccaccccg ctcgaacgcc tcgacttcgc catgctcacg cacaaggccg aactcggcat
     1261 cgaccccttc accgacaaga agacggcgac gcgcacccgc gacaccacga agccgggctg
     1321 gtacgaccac aagggcttct tcgaccgcaa gctcgaagac ccctcggtct acgacatggg
     1381 caaggagaag gagccgcgcg accacctccg catgccgaag ccctacctca agcccgagtg
     1441 gaaggtcgcg ctcacgacgt tcctcttggg gagcgtcggc aacgagggcg cgaacatccc
     1501 cacctcgctc ttctaccagc cgaacgagca gcagaaggcg atccaggacg gctggtgggt
     1561 catcaagaag tacaactgca tgggctgcca caacgtgcag gtcggccagc agtccgacct
     1621 ctcgacgctg cccttctatc aggagggcgg cgcgctcggc aaagaacaac tgccgcccgg
     1681 cctgacgacc gaaggcgcgc gcgtcgatcc cgactggctc ctccgcttcc tgaccgaccc
     1741 ttcgctgagc ggcgtctcgc tcgacgcgcg cagcctcgcc cgtccggggc agatcagcgg
     1801 cgccaacccc gcgctcgccg cgcgtggaca gcagcccgcg aacggaaccc ccgtcggcaa
     1861 cccgccgggc gcacaaccga acgccgcggc gcagcccaac gcgatctcga acgcgcacgc
     1921 cgcgctcaag ccgcagccgg gcgcgccgcg caacggcgtc cgcacgtacc tgcgcgcgcg
     1981 gatgccgacc ttcaacttct cgccgaacga gctgcgcacg ctcgtccgct tcttccaggc
     2041 cgtctccgcg cagcacgagc ctttcatcaa ggagcaggcc gagccgctca cggacgcgga
     2101 gagggacctg gcccgcgcgc tcttcacctc gcaggccgcg ccctgcctga agtgccacgt
     2161 cacgagcgag ggcgagcaag gcaagaccgc gccgaacttc ctgatcgcgg gcgagcgcct
     2221 caagtccgac tggacatacc gctggctgct cgacccgcaa cgcatcatgc ccggcacggc
     2281 gatgccttcg gagctgttca aacgcgaggg cgagcgctgg gtcttcaacg gcccgctgcc
     2341 gccctccgcc gcggactatc agggagacca cgcacggctg ctcgtccgct acctgctcca
     2401 actgacgccc gaagagcagg cgcgctcggc ccgctcggcg gcctccgcgc ccgccgtcac
     2461 ccccgccgcg cccgccgcgg cacagcccgc gacgcccgtc gcctcgcacc gccgtgcgaa
     2521 gacgccgcgc gccgcagccg cgcgcccgac cgagaacgcc ggacgccgcg tgcgccgcgc
     2581 gcgcaggcag caagcgtcca tgcgccccgc gcccgcgcgc cgcgcgcgca gaggctcaag
     2641 aggtcaggcc gccgcggcga ccgcgcgtcc gcggcgcgcg cccgatttct cgcgctgaag
     2701 gtttagagtt atcatagcgt cacatcattc gcaggaggtt ttcccaaaga tgcaatccaa
     2761 acgcgcgcgt tttctcgcgg cgctcatcgc ggccctctgc ctcctcgcct tcaccgccgc
     2821 gtgcggcagg gacgaggacg ccgaagaccc ggaagggccg gagggacaga cggaagagac
     2881 tgatgtcgtg ctgacgccct tcaagcccac gggcaacgag ggcgccatcg ccggcaccgt
     2941 cagcttcgcg ggcacgcccc cgacgccgcc cgacatctcc atgctcgccg acgccttctg
     3001 cgcctcggcc aaccagaacc cgaaggcgga ggacacggtc gtcaacaacg gcaccctgca
     3061 aaacgtcttc gtctacgtca aggacggc
//
LOCUS       DBNGOH010000777         5374 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4698720, whole genome shotgun sequence.
ACCESSION   DBNGOH010000777 DBNGOH010000000
VERSION     DBNGOH010000777.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5374)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5374)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5374
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4698720"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1172
                     /gene="hslU"
                     /locus_tag="AC159P_14925"
     CDS             <1..1172
                     /gene="hslU"
                     /locus_tag="AC159P_14925"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014098676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HIR3696792.1"
                     /translation="VKVEASKFTEVGYVGRDVESMIRDLTEIAVDMVRAEKIDEIADK
                     AELNAEERILDLLLPPRAASHFNFDAPEEDPAPYAGSQGAAQGTAQGRDDQSQRTREK
                     LRAQLREGKLDNRLVEIEVREKSFPTIEVAGPQGVEEMGINMKDMLGNLFQGRTKRQR
                     MRVDEAMEYLLQEEEEQLVDMDAVARAALERVESSAIIFLDEIDKIAGRESASGGPDV
                     SREGVQRDILPIVEGTTVNTRYGMVKTDHILFIAAGAFHVSKPSDLIPELQGRFPIRV
                     ELESLTLEDFKRILTEPKNSLVKQYKALLETEGIELDFTDDAVDAVARFAVRVNETTE
                     NIGARRLHTIMEKVLEEISFAGPDLEPKRQVVDADYVTRELADIVKDQDLSRFVL"
     gene            1249..2337
                     /locus_tag="AC159P_14930"
     CDS             1249..2337
                     /locus_tag="AC159P_14930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HIR3696793.1"
                     /translation="MTFAFCLLPFAFCLPGCGKRRPPQPPVEGVPQRTELLSGVQRGN
                     QIILDWPAPLRNAPDESVQSIRRVDVYRLAESSDDPLALTEEEFSSRATLIGSVPFET
                     IRAAAPGGAVSYTDELTLSDPVRLRYAVRYVNASNQRASFSNFLIIEPATGVSRPPTL
                     TAPAEVTQNAIEFVWDAPAANMDGTRPANVLGYNVYRFARAEKQPAREPLNGARPLSA
                     TRFVDQSFSFGEEYVYVVRAVSLGTGGLPVESLNSNAISVAPRDTFPPAPPGAPTAAA
                     PQDGGRISIFFPANTESDVVGYNIFRATDEGLPRSLWTKLNRAPLTRTTFQDETVQSG
                     VKYFYYVTAIDNAGNESRPSEVDSETAP"
     gene            2334..3143
                     /locus_tag="AC159P_14935"
     CDS             2334..3143
                     /locus_tag="AC159P_14935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013377384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HIR3696794.1"
                     /translation="MNHTRICRYLDEETARPRFGLVQEEEIKPLPLHFSYGTADPHEA
                     VHADAAARPFGMEGARLLAPVTPSKIVCVGRNYREHAAELGNPLPTEPLLFLKPPSAI
                     IGPCETIELPFVSERIEHEGELAVVIGRKVRRLADAEDPLSYVYGYTCLNDVTARDLQ
                     RRDVQFTRAKSFDTFCPVGPHVVKGLDPTNLSVRTRVNGETRQDGRTSAMAFPCAALV
                     RYISHVMTLEPGDLIATGTPAGVGPLSDGDTVEVEVEGVGVLRNPVRRTPR"
     gene            3156..3677
                     /locus_tag="AC159P_14940"
     CDS             3156..3677
                     /locus_tag="AC159P_14940"
                     /inference="COORDINATES: protein motif:HMM:NF016085.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HIR3696795.1"
                     /translation="MSRRLLTVRSLKYDGRVHREWRAELLRAEGMLLVVEGEFEEEVS
                     HPLLGTIRAGTVSTEFYWADRWYSVFRFREPSGELRYYYCNINTPARLHEATLTFVDL
                     DIDVLVAPDYSCRVLDEDEFDAHEKLYGYPPEFRRRAHRALEEVCALVRARRFPFGEG
                     PDKAAARPGGPVA"
     gene            3726..5294
                     /locus_tag="AC159P_14945"
     CDS             3726..5294
                     /locus_tag="AC159P_14945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011525493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HIR3696796.1"
                     /translation="MKPEPIEVQQSKLDELRERKRLAAAGGGPSRVARQHEAGKWTAR
                     ERVEFFLDEGTFEEFDELVVHRTHDFGLDRQRFPGDGVVTGHGLCEGRRVFVFAQDFT
                     VFGGSLSETHAAKICKVMDLAMRVGAPVVGLNDSGGARIQEGVVSLGGYADIFLRNTL
                     ASGVVPQISCIMGPCAGGAVYSPAITDFNVMVKNTSYMFITGPDIIRTVTHEEVSKEE
                     LGGADTHNRVSGVAHFAAETDEHALLLVRELLSFLPSNNMEDPPRAATADPPDRRDAA
                     LNEIIPESSNQPYDIRDVIHTVVDERHFFEVHEHYAPNIVCGFARLDGRSVGVVANQP
                     AFLAGVLDIDASVKAARFVRFCDCFNIPLVVFEDVPGFLPGVRQEHLGIIRHGAKLLY
                     AFAEATVPKITVITRKAYGGAYCVMASKHIRTDINFAWPTAEIAVMGAEGAVGLLYRR
                     EIGEAVDKDEARRARVAEFEEKFANPYVAAERGFIDEIIEPAETRPKLIRALRLIENK
                     RDSNPPKKHGNIPL"
BASE COUNT          981 a         2032 c         1580 g          781 t
ORIGIN      
        1 tcgtcaaggt cgaggcctcg aagttcacgg aggtcggcta cgtcggcagg gacgtggaga
       61 gcatgatccg cgacctcacg gaaatcgcgg tggacatggt gcgcgccgag aagatcgacg
      121 agatcgcgga caaggccgaa ctgaacgccg aagagcgcat cctcgacctc ctgctcccgc
      181 cgcgcgccgc ctcccacttc aacttcgacg cgccggaaga agaccccgcc ccgtacgcag
      241 ggtcgcaggg cgccgcacag ggcaccgcgc aggggcgcga cgaccagtcg cagcgcacgc
      301 gcgagaaact gcgcgcccaa ctgcgcgagg gcaagctcga caaccgcctc gtcgagatcg
      361 aagtccgcga gaagagcttc ccgaccatcg aggtcgcggg cccgcagggc gtcgaggaga
      421 tgggcatcaa catgaaggac atgctcggca acctcttcca gggccgcacc aagcgccagc
      481 gcatgcgcgt ggacgaggcc atggagtacc tcttgcagga ggaggaggag caactggtgg
      541 acatggacgc cgtcgcccgc gccgcgctcg aacgcgtcga gtcctcggcc atcatcttcc
      601 tcgacgagat cgacaagatc gcgggccggg agtcggcttc gggcgggccg gacgtctcgc
      661 gcgagggcgt ccagcgcgac atcctcccca tcgtcgaagg caccaccgtc aacacccgct
      721 acggcatggt caagacagac cacatcctct tcatcgccgc cggcgccttc cacgtctcca
      781 agccctcgga tttgatcccg gagctacagg gccgcttccc catccgcgtc gaactcgaat
      841 ccctcaccct cgaagacttc aaacgcatcc tcacagagcc gaagaactcg ctcgtcaaac
      901 agtacaaggc gctgctcgaa accgagggca tcgaactcga cttcacggac gacgccgtgg
      961 acgccgtcgc gcgcttcgcc gtgcgcgtca acgagacgac cgagaacatc ggcgcgcgcc
     1021 gcctgcacac gatcatggag aaggtcttgg aagagatcag cttcgccggc cccgacctcg
     1081 aacccaagcg gcaggtcgtg gacgcggact acgtcacgcg cgaactcgcg gacatcgtca
     1141 aagaccagga cctcagccgc ttcgtcctct aacgaattga aggcacacgg cacacggcac
     1201 acggcggcag gcaaaagtgt ggacgcgctc cgcgcgaggt tcgtcttttt gacttttgcc
     1261 ttctgccttc tgccttttgc cttttgcctt cccggctgcg ggaagcgtcg cccgccgcag
     1321 ccgcccgtcg aaggcgtccc gcaacgcacg gagctgctct ccggcgtcca gcgcggcaac
     1381 cagatcatcc tcgactggcc cgcgcccctg cggaacgcgc ccgacgagag cgtccagagc
     1441 atccgccgcg tggacgtcta ccgcctcgcc gagagcagcg acgacccgct cgcgctcacc
     1501 gaagaggagt tctcttcgcg cgccacgctc atcggctccg tccccttcga gacgatccgc
     1561 gccgcggccc ccggcggcgc cgtcagttac acggacgagc tgacgctctc cgaccccgtc
     1621 cgcctgcgct acgccgtccg ctacgtcaac gcctcgaacc agcgcgcctc cttctccaac
     1681 ttcctcatca tcgaacccgc gaccggcgtc agccggccgc ccacgctcac agcccccgcc
     1741 gaggtcacgc aaaacgccat cgagttcgtc tgggacgcgc ccgccgcgaa catggacggc
     1801 acgcggcccg cgaacgtcct cggctataac gtctaccgct ttgcgcgcgc ggagaaacag
     1861 cccgcacgcg agccgctcaa cggcgcgcgg cccctctccg cgacgcgttt cgtggaccag
     1921 tccttcagct tcggcgaaga gtacgtctac gtcgtccgcg ccgtctcgct cggcaccggc
     1981 gggctgcccg tcgagagcct gaattccaac gccatctcgg tcgcccccag agacaccttc
     2041 ccgcccgccc cccccggcgc gccgaccgcc gccgccccgc aagacggcgg gcgcatctcc
     2101 atcttcttcc ccgcgaacac ggagagcgac gtggtcggct ataacatctt ccgcgcgacc
     2161 gacgaggggt tgcccaggag cctgtggacc aagctcaacc gcgcccccct cacgcgcacc
     2221 acctttcagg acgagacggt gcaatccggc gtcaaatatt tttactacgt gaccgccatc
     2281 gacaacgccg gcaacgagag ccggccgtcc gaagtggact cggagaccgc cccatgaacc
     2341 acacgcgcat ctgccgttac ctcgacgaag agacggcacg gccccgcttc ggcctcgtcc
     2401 aagaggagga gatcaagccc ctccccttgc acttctcgta cgggacagcc gacccacacg
     2461 aggctgtcca tgcggacgcc gccgcgcgcc cgttcggcat ggagggcgcg cggctgctcg
     2521 cgcccgtcac cccttcgaag atcgtctgcg tcggtcgcaa ctacagggag cacgcggcgg
     2581 aactcggcaa ccccttgccc acagagcccc tcctcttcct caaaccgccc tccgccatca
     2641 tcggcccctg cgagacgatc gagctgccct ttgtctccga aagaatcgaa cacgaaggag
     2701 aactcgccgt cgttatcgga cgcaaagtcc ggcgcctcgc ggacgccgaa gaccctctct
     2761 cctacgtcta cggctacacc tgcctgaacg acgtgacggc gcgcgaccta cagcggcgcg
     2821 acgtgcagtt cacgcgcgcc aagtctttcg acaccttctg ccctgtcggc cctcacgtgg
     2881 tcaagggcct cgaccccaca aacctctccg tacggacgcg cgtcaacggc gagacacggc
     2941 aggacgggcg gacgagcgcg atggccttcc cctgcgccgc gcttgtccgc tacatctcgc
     3001 acgtcatgac gctcgaaccc ggcgacctca tcgccacggg cacgcccgcg ggcgtcgggc
     3061 ctctctcgga cggcgacacg gtcgaggtcg aagtcgaggg cgtcggggtg ctccgcaacc
     3121 ccgtgcgccg cacgccgcgc tgacccttta ctcctttgag ccgccgcctg ctcacagtcc
     3181 gatcgctgaa gtacgacggg cgcgtccacc gcgagtggcg cgccgagttg ctgcgcgcgg
     3241 aagggatgct cctcgtcgtc gagggcgagt tcgaagagga ggtcagccac ccactgctcg
     3301 gcaccatccg cgccggcaca gtgagtacgg agttctactg ggccgaccgc tggtacagcg
     3361 tcttccgctt ccgcgaaccc tcgggcgagc tgcgctacta ctactgcaac atcaacacgc
     3421 ccgcgcgcct ccacgaagcg acgctgacct tcgtcgatct cgacatcgac gtgctcgtcg
     3481 cgccggatta ctcctgccgc gtcctcgacg aggacgagtt cgacgcgcac gaaaagctct
     3541 acggctaccc gcccgagttc cgccgccgcg cacaccgcgc gctcgaagag gtctgcgccc
     3601 tcgtccgcgc ccgccgcttc cctttcggag agggaccgga caaagccgcc gcacgcccag
     3661 gcggccccgt tgcgtaaacc cgcctgcatc cttcataatt cgccgcgcca atcaacaact
     3721 tgaacatgaa gccggaaccc atcgaagtcc aacagtcgaa gctcgacgag ttgcgcgagc
     3781 gcaagcggct cgccgccgcg gggggcggcc cttcgcgcgt cgcgcgccag cacgaggcgg
     3841 gcaagtggac tgcgcgcgag cgcgtcgagt tcttcctcga cgagggcacg ttcgaggagt
     3901 tcgacgagct ggtcgtccac cgcacgcacg acttcggcct cgaccgacag cgcttccccg
     3961 gcgacggcgt cgtcacaggc cacggcctct gcgaaggccg ccgcgtcttc gtcttcgcgc
     4021 aggacttcac ggtcttcggc ggctcgctct ccgagaccca cgccgccaaa atctgcaagg
     4081 tgatggactt ggcgatgcgc gtcggcgcgc ccgtcgtcgg cctcaacgac tcgggcggcg
     4141 cgcgcataca ggagggcgtc gtctctctgg gcggctacgc ggacatcttc ctccggaaca
     4201 cgctcgcctc gggcgtcgtc ccgcagatca gttgcatcat ggggccttgc gcgggcggcg
     4261 ccgtctacag cccggccatc acggacttca acgtcatggt gaagaacacc tcctacatgt
     4321 tcatcaccgg ccccgacatc atccgcaccg tcacgcacga agaggtctcg aaggaagaac
     4381 tcggcggcgc ggacacgcac aatcgcgtct ccggcgtcgc gcacttcgcc gccgagacgg
     4441 acgagcacgc gctcctgctc gtccgcgagc tgctctcctt cctcccctcg aacaacatgg
     4501 aagacccgcc gcgcgccgcc accgccgacc cgcccgaccg ccgggacgcg gcgctcaacg
     4561 agataatccc ggaatcctcg aaccagccct acgacatccg cgacgtgatc cacacggtcg
     4621 tggacgagcg ccacttcttc gaagtccacg agcactacgc gcccaacatc gtctgcggct
     4681 tcgcgcggct cgacgggcgc agcgtcggcg tcgtcgccaa ccagccagcc ttcctcgcag
     4741 gggtgctcga catcgacgcc tccgtcaagg ccgcgcgctt cgtacgcttc tgcgactgct
     4801 tcaacatccc cctcgtcgtc ttcgaggacg tgcccggctt cctgcccggc gtacggcagg
     4861 agcacctcgg catcatccgc cacggcgcca agctcctcta cgccttcgca gaggcgaccg
     4921 tccccaagat caccgtcatc acgcgcaagg cctacggcgg cgcctactgc gtcatggcct
     4981 cgaagcacat ccgcacagac atcaacttcg cctggcccac cgcagagatc gccgtcatgg
     5041 gcgcagaagg ggccgtcggc ctcctctacc gccgcgagat cggcgaggcc gtggacaagg
     5101 acgaagcgcg ccgcgcacgc gtcgcggagt tcgaggagaa gttcgccaac ccctacgtcg
     5161 ccgccgagcg cggcttcatc gacgagatca tcgaacccgc cgagacgcgc cccaaactca
     5221 tccgcgccct ccgcctcatc gaaaacaaac gcgactccaa cccgcccaaa aagcacggca
     5281 acatcccgct ctgaagcagc aggcagtagg cagcgggcag taggcagtca agacggaaga
     5341 gcgaagaggc tcgaaaggta aagtcccgag cctc
//
LOCUS       DBNGOH010000778         6832 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1035111, whole genome shotgun sequence.
ACCESSION   DBNGOH010000778 DBNGOH010000000
VERSION     DBNGOH010000778.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6832)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6832)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6832
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1035111"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(160..909)
                     /locus_tag="AC159P_14950"
     CDS             complement(160..909)
                     /locus_tag="AC159P_14950"
                     /inference="COORDINATES: protein motif:HMM:NF018129.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031411 - gas vesicle [Evidence IEA];
                     GO_process: GO:0031412 - gas vesicle organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GvpL/GvpF family gas vesicle protein"
                     /protein_id="HIR3696797.1"
                     /translation="MKLYAYCVCDGVEAAAFEGERGVSGAAAKLVECGGGLAAFVSES
                     EEGQPVAVTPENVRAHNRVNARLLAHTTPLPFRFGTLITEVRLKTYVTANVEALREAL
                     ARVGGCVEMGVKIMSVAGAVVDEAAVDDEVNEAETPPSLTVANRAGRGTAFLLAKRRE
                     ILGGEEARSRAEETAALLAARVGEVVRDSRVRVLPSRALLVRAAHLVERARLEESRAR
                     VRPLEAERAGGLSFLTSGAWPPYSFSEIRST"
     gene            complement(913..1179)
                     /locus_tag="AC159P_14955"
     CDS             complement(913..1179)
                     /locus_tag="AC159P_14955"
                     /inference="COORDINATES: protein motif:HMM:NF012944.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0012506 - vesicle membrane [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gas vesicle protein"
                     /protein_id="HIR3696798.1"
                     /translation="MADEVFELGEDEELSLLETLDHVLDHGLVVAGEVTIAVADVDLI
                     FVGLNVLLGSTETINRVLGGRERRAELLSEGATAAGEVRETPER"
     gene            complement(1261..2163)
                     /locus_tag="AC159P_14960"
     CDS             complement(1261..2163)
                     /locus_tag="AC159P_14960"
                     /inference="COORDINATES: protein motif:HMM:NF018129.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031411 - gas vesicle [Evidence IEA];
                     GO_process: GO:0031412 - gas vesicle organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GvpL/GvpF family gas vesicle protein"
                     /protein_id="HIR3696799.1"
                     /translation="MSARSPNAKAARKPGRERRPAALYVYCVGAREELAPLVEGAGVA
                     PIETGAGVELVEGGGLAAVVSDVPLADYGEDALRERLQDPAWMATRAMRHEAAVEHFA
                     RRAAVVPLRFGTIYLSREAVEKMLAERGAQLRSIISRVEGREEWGVNVYADRLKTREA
                     VARVSPRLLEMDEQAARSTPGQAYLLRKKIDALRADEARAETKRAAASIEDALRAASE
                     GAARLRVLKDEASEHGDLAAKLAFLVPRDAFEGFRAEAERLAQQFAPLGLRLELTGPW
                     PAYNFVVFDDEEGKRQKAKGKNQQ"
     gene            complement(2229..2702)
                     /locus_tag="AC159P_14965"
     CDS             complement(2229..2702)
                     /locus_tag="AC159P_14965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HIR3696800.1"
                     /translation="MSIEARVVVNSDPRPLYGETLYLLDADPILLAMAEVNESDPVQV
                     RTHREHPRLRMLAGLMNARRLAAYRLGTDIAIVLEQSKPLWETHVVRTAQTDERGRAV
                     FDRLAPGHYWLMCLSERDGKLAFWNPRVTIESARTEAVVLDQSNALTFGDSPARG"
     gene            complement(2846..5149)
                     /locus_tag="AC159P_14970"
     CDS             complement(2846..5149)
                     /locus_tag="AC159P_14970"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014433.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA family ATPase"
                     /protein_id="HIR3696801.1"
                     /translation="MTKKGAKRGDRDGKAARAPKGSKKNAARPSSKKSASPRSPERAP
                     RRQTKRAARPPKVSDEEAKTSAVAKVSKASKVPKSSAASEASEAASASRAADTLPDTR
                     YLFFGGKGGVGKTTAACAAALLLLDRAAKGERILLFSTDPAHSLSDSLGTEVGDRLVE
                     VARRGKSADAPLLFAREMDAARALDAFKTEHRAVLAEIADRGTMLDEADINDLLDLSL
                     PGMDEVMALFELSEVERADVYARVVVDTAPTGHTARMLRLPAVFTRWIGALDRMSEKH
                     RYMVAHFVRGRAAREDEVDRFLRDLEARVRAARAMLFDAKHASFTLVTIPEALAVEES
                     ARYLRTLREEGAPVTDLVINRVEHKRGACPFCRARAAAQTRWLARLESDFKPLRLRRV
                     PLFPGEVRGPEVLRRFARAVWDGEMGGRGDGEKKKTAASSSSLRIATPSTRLRASSSP
                     RLPVSPSPRLLVFGGKGGVGKTTAAAAAALALARSDKKARVLCFSIDPAHSLSDSFDE
                     EIGELKRGVAGLKNLDAMEIDPARRFEELKERYRAWTDELFESLTGGSRFEVQFDREA
                     MRELVSLAPPGIDEIAALSTISDLVEDGSYTTIVLDTAPTGHLLRFLELPEVALAWVR
                     TFIKLLLKYKNVVRWGGVAEELVALSKSIKRVAALLADPANTEFVGVAIPERMSLEET
                     ARLAESLTRLRVPLKRLLINNVVTEEAAAGCDFCDARRRSQTGVVKEFKRKFSRVAEL
                     YAAPQQPHEVRGPELLREHFERWVALD"
     gene            complement(5146..5484)
                     /locus_tag="AC159P_14975"
     CDS             complement(5146..5484)
                     /locus_tag="AC159P_14975"
                     /inference="COORDINATES: protein motif:HMM:NF016976.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gas vesicle protein GvpG"
                     /protein_id="HIR3696802.1"
                     /translation="MFLIDDILLAPVNGFKFIMKQIQQLADKELNDESLIKEQLLELQ
                     MRLELEEISDEEYVEAERELFARLRAIKQRQLEAMGEVHTADKSSVVIETGGDDAGGA
                     FWEPGGGDER"
     gene            complement(5504..5671)
                     /locus_tag="AC159P_14980"
     CDS             complement(5504..5671)
                     /locus_tag="AC159P_14980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696803.1"
                     /translation="MWFLLMPLDYLWDGTLDSLNVEGVARRVTVAISVWFFFGWAIAS
                     SAGEGSPRDNC"
     gene            complement(5821..6633)
                     /locus_tag="AC159P_14985"
     CDS             complement(5821..6633)
                     /locus_tag="AC159P_14985"
                     /inference="COORDINATES: protein motif:HMM:NF018129.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031411 - gas vesicle [Evidence IEA];
                     GO_process: GO:0031412 - gas vesicle organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GvpL/GvpF family gas vesicle protein"
                     /protein_id="HIR3696804.1"
                     /translation="MSAETDQISAAAATPPPEGKYVYCIIESKEPRSFGPHGIGGRGD
                     DVYTVHYDGLAAVVSNTPIMVYDPTRENVFAHEQVNETVMREFTVLPMAFGVLFRTEE
                     DILELLRGTYDALRDVLAKMAGKVEFGLKVNWDRERVIAEIEEESEEIGNLKEQITSR
                     ASGSTYFARMQLGRLIEGALTEKSDGYVRDIYDALRDTAVASRANKPIGDKMIMNAAF
                     LIERDREAEFDNKVKDIAAKYEGKLAFKYTGPWPPYNFVHIRLKLERGEGAA"
BASE COUNT         1058 a         2345 c         2258 g         1171 t
ORIGIN      
        1 ccggaacggg ggaatcaggg gggtgctgca tgctcagagt aacccgcgcg ccgctcccgt
       61 gtccacaact ttcacgaacg cgcgccgggc cgcgggcggc gaagaagaga agggcgcgtg
      121 aatcctgaac gcgcgcttcg gactcgaccg cgggccggcc taagttgaac ggatttcgct
      181 gaaactgtac gggggccacg cgccgctcgt caagaagctg aggccgcccg cgcgctccgc
      241 ttcgagcgga cgcacccgcg cgcgagactc ttcgagccgc gcccgctcga ccagatgcgc
      301 cgcgcgcacc agcagcgcgc gcgaaggcag cacgcgcacg cgcgagtcgc gcaccacttc
      361 gcccacgcgc gccgcgagca gcgccgccgt ctcctcggcg cgcgagcgcg cctcttcacc
      421 gcccagaatc tcgcgccgct ttgccagcag gaacgccgtg ccccgcccgg cccgattcgc
      481 aaccgtcaac gacggcggcg tctctgcttc attcacttca tcatcgacag ccgcctcatc
      541 cacgaccgcg cccgcgaccg acatgatctt gacgcccatc tcgacgcagc cgccgacgcg
      601 cgcgagcgcc tcgcgcagag cctccacgtt cgccgtcacg taagtcttga gccgcacctc
      661 cgtgatgagc gtgccgaagc ggaaggggag cggcgtcgtg tgcgccagca ggcgcgcgtt
      721 gacgcggttg tgcgcgcgga cgttttcggg cgtgacggcg accggctggc cctcttccga
      781 ctcgctgacg aacgccgcga gaccgccgcc gcactcgacc agcttcgccg ccgcgccgct
      841 caccccgcgc tcgccctcga acgccgcggc ctcgacgccg tcgcacacgc aataggcgta
      901 caacttcatc gctcaacgct ccggcgtctc gcgcacctcg cccgcggcgg tcgcgccctc
      961 cgacagcagc tccgcgcgtc gctcgcgccc gccgagcacg cggttgatcg tctccgtcga
     1021 gccgagcagc acgttcagcc ccacgaagat gaggtcaacg tcggccaccg caatcgtcac
     1081 ctcgcccgcg accaccagcc cgtgatccag cacgtggtcg agcgtctcca acagcgacag
     1141 ctcttcgtcc tcgccaagct cgaacacttc gtcagccata aactcttcga gaaggcaaaa
     1201 ggcaaaaggc aaaaggcaaa gggcaaaagg gcgggcgcgc tgcgcgcgaa ctcaatgctt
     1261 tcattgttga tttttgcctt ttgccttttg ccttttgcct tcttcgtcgt cgaagacgac
     1321 gaagttgtac gcgggccacg ggccggtcag ctccaaccgc aggccgagcg gcgcgaactg
     1381 ttgcgcgagc ctttcggcct cggcgcggaa cccttcgaag gcgtcgcgcg ggacgaggaa
     1441 ggcgagcttg gcggcgaggt cgccgtgctc cgacgcctca tccttgagca cgcgcaggcg
     1501 cgcggcgccc tcgctcgcgg cccggagcgc gtcttcgatc gaggcggcgg cgcgcttcgt
     1561 ctcggcacgc gcctcgtcgg cgcgcagcgc gtcgatcttc ttgcgcagca gatacgcctg
     1621 gccgggcgtg gagcgcgcgg cctgctcgtc catctccagc aggcgcgggc tgacgcgcgc
     1681 caccgcctcg cgcgtcttca agcggtcggc gtagacgttg acgccccact cctcgcgccc
     1741 ctcgacgcgg ctgatgatgg agcggagctg cgcgccgcgc tcggcgagca tcttctcgac
     1801 cgcctcgcgg ctgaggtaga tcgtgccgaa gcgcagcggc acgaccgccg cgcgccgcgc
     1861 gaagtgctcg accgcagcct cgtgccgcat cgcgcgcgtc gccatccacg cggggtcttg
     1921 cagccgctcg cgcagggcgt cttcgccgta gtccgcgagc ggcacgtccg acacgaccgc
     1981 ggccagcccg ccgccctcga ccagctcgac gcccgcgccc gtctcgatcg gcgctacgcc
     2041 ggcgccttcg acgagcggcg ccagctcttc gcgcgcgccg acgcagtaga cgtagagggc
     2101 cgcgggccgc cgctcgcgcc ccggctttcg cgccgccttc gcgttcggac ttcgcgcgct
     2161 cattgttttt ttcgccgcgg acgaatcatg acaggcacgc tgcttttcat ccatcgccac
     2221 gcgcttcttc atccgcgcgc gggcgagtcg ccgaacgtca gcgcgttgga ctggtcgagc
     2281 acgaccgcct ccgtccgcgc gctctcgatt gtgacgcgcg ggttccagaa cgcgagcttg
     2341 ccgtctcttt cggagaggca catcagccag tagtggcccg gcgcgagccg gtcgaagacg
     2401 gcgcggcccc gctcgtccgt ttgcgccgtc cgcacgacgt gagtttccca gagcggcttc
     2461 gactgttcaa gcacgatggc gatgtccgtc ccgagccggt aggccgcgag gcgccgcgcg
     2521 ttcatcagcc ccgccagcat gcgcagccgc gggtgctcgc ggtgcgtccg cacctggacg
     2581 gggtcgcttt cgttgacctc ggccatcgcc aacagtatcg ggtctgcgtc gagcaggtac
     2641 agcgtctcgc cgtagagggg ccgcgggtcg ctattgacga cgacgcgcgc ctcgattgac
     2701 aacgagccgc cttcgtctct attcgttgca ccgcacgccg cgcacagtaa cgtgcaggcg
     2761 agtgcgagca tgagaggtaa agtgtttgag cggttgtacg ccgcctgact cagcatgacg
     2821 gacacgcgtg tgatgggttc ggtcttcagt cgagagcgac ccagcgctcg aagtgttcgc
     2881 gcagcaactc cggcccacgg acttcgtgcg gctgctgcgg cgcggcgtag agttcggcca
     2941 cgcggctgaa cttgcgcttg aactctttga cgacgcccgt ctgcgaccgc cgccgcgcgt
     3001 cgcagaagtc gcacccggcg gcggcctctt ccgtcacgac gttgttgatg aggagtcgct
     3061 tcagcggcac tctcaaacgc gtcaaacttt cggcgagccg cgccgtctcc tcgaggctca
     3121 tgcgctcggg gatggcgacg ccgacgaact cggtgttcgc cgggtcggcc aagagcgccg
     3181 cgacgcgctt gatgctcttc gagagcgcga ccagctcttc ggccacgccg ccccagcgta
     3241 ccacgttctt gtacttgagg agcagcttga tgaaagtccg cacccatgcg agcgccactt
     3301 cgggcagctc cagaaagcgc aggaggtggc ccgtcggcgc cgtgtcgagc acgatggtcg
     3361 tgtagctccc gtcttcgacg aggtcgctga tggtcgagag cgcggcgatc tcgtcgatgc
     3421 ccggcggcgc gagcgagacc agctcgcgca tcgcctcgcg gtcgaactgc acctcgaagc
     3481 gcgagccgcc cgtcagagac tcgaacagct cgtccgtcca agcgcggtag cgctccttca
     3541 gctcctcgaa ccgacgcgcc gggtcgatct ccatcgcgtc cagattcttc aagccggcga
     3601 cgccgcgctt cagctcgccg atctcctcgt cgaaactgtc cgagagcgaa tgcgccgggt
     3661 cgatggagaa acagaggacg cgcgccttct tatcgcttcg cgcgagcgcg agcgccgccg
     3721 ccgccgccgc ggtcgtcttc ccgacgccgc ccttgccacc gaacacgagc agacggggag
     3781 acggggagac ggggagacgg ggagacgaag aagcacgtaa gcgagttgaa ggagtggcga
     3841 tgcgcagact cgaagacgaa gcggctgttt ttttcttctc cccgtctccc cgtcccccca
     3901 tctccccgtc ccagactgcg cgcgcgaagc ggcggagcac ctcggggccg cgcacctctc
     3961 cggggaagag ggggacgcgg cgcaggcgca gaggcttgaa gtcgctttcg agtcgcgcga
     4021 gccagcgcgt ttgcgcggcg gcgcgggcgc ggcagaaggg gcaggctccg cgcttgtgct
     4081 cgacgcggtt gatgacaaga tcggtgacgg gcgctccctc ttcgcggagc gtgcggaggt
     4141 agcgcgcgct ctcttcgacg gccagcgctt cggggatggt gacgagcgtg aagctcgcgt
     4201 gcttcgcgtc gaagagcatc gcgcgcgcgg cgcggacgcg ggcttcgagg tcgcgcagga
     4261 agcggtcaac ctcgtcctcg cgcgcggcgc gcccgcggac gaagtgcgcg accatgtagc
     4321 ggtgcttctc gctcatgcgg tcgagcgccc cgatccagcg cgtgaagact gcgggcagcc
     4381 gcagcatgcg ggcggtgtgc cccgtgggcg cggtgtccac gacgacgcgg gcgtaaacgt
     4441 cggcgcgctc gacctcgctc agctcgaaga gcgccatcac ctcgtccatg ccgggcaggc
     4501 tgaggtcgag caagtcgttg atgtctgcct cgtcgagcat tgtgccgcgg tcggcgatct
     4561 ccgcgagcac ggcgcggtgc tcggtcttga acgcgtcgag cgcgcgcgcg gcgtccatct
     4621 cgcgcgcgaa gaggagcggg gcgtccgcag acttgccgcg ccgcgcgact tcgacgaggc
     4681 ggtcgccgac ctctgtgccg aggctgtccg agagagaatg cgcggggtct gtcgagaaga
     4741 ggaggatgcg ctcgcctttg gcggcgcggt cgagcaatag gagcgcggcg gcgcaggccg
     4801 cggtcgtctt gccgacgccg cccttgccgc cgaagaaaag ataacgcgtg tcggggagtg
     4861 tatccgcggc gcgcgacgcg ctcgcggcct ctgatgcttc ggaggccgct gaagatttag
     4921 ggactttgga tgctttcgaa actttagcga ctgcggaggt tttggcttct tcgtcggaga
     4981 ctttgggcgg gcgcgcggcg cgtttcgtct gacgacgcgg ggcgcgctcg ggcgaacgag
     5041 gcgaggcgct cttcttgctc gaaggtcgcg cggcgttctt cttcgagcct ttgggcgcgc
     5101 gcgcggcctt gccgtctcgg tcgccacgtt tcgcgccctt cttcgtcaac gctcgtcgcc
     5161 tccgcccggc tcccagaacg cgccgcccgc gtcgtctccg cccgtctcga tcaccaccga
     5221 ggatttgtcc gccgtgtgca cctcgcccat cgcttcgagt tgccgctgct tgatggcgcg
     5281 cagccgcgcg aacagctcgc gctcggcctc gacgtactcc tcgtccgaaa tctcttccag
     5341 ctccagccgc atctgcaact cgagcaactg ctccttgatc agagactcgt cgttcagctc
     5401 cttgtcggcg agctgctgta tctgcttcat gatgaacttg aagccgttga cgggcgcgag
     5461 caggatgtcg tcaattaaaa acatggcggg cggatgcgat ccctcagcag ttatcccgcg
     5521 ggctaccttc accggcggaa gacgctatgg cccacccaaa gaagaaccag accgagatgg
     5581 cgaccgtcac gcggcgggca acgccttcga cgttaaggct gtcgagggtg ccgtcccaca
     5641 gataatccag cggcatgagg agaaaccaca tcacgactcc cattaaggcc cctgccaggc
     5701 tgcaatggcg aaacaggggc caccgcccct tagctttaag gcgcagcagt ttccttgcgt
     5761 tccactccgc gagagtcgtt aacggatttc gcatactccc tccgccggct tcgacgaacc
     5821 ttacgccgcg ccctcgccgc gctccaattt gaggcggatg tggacgaagt tgtagggggg
     5881 ccaggggccg gtgtacttga aggccagctt gccttcgtac ttggcggcga tgtctttgac
     5941 cttgttgtcg aactcggcct cgcggtcgcg ctcgatgagg aaggcggcgt tcatgatcat
     6001 cttgtcgccg atgggcttgt tggcgcgcga ggcgaccgcc gtgtcgcgca gggcgtcgta
     6061 gatgtcgcgg acgtagccgt cggacttttc ggtgagcgcg ccctcgatca ggcgcccgag
     6121 ctgcatgcgc gcgaagtagg tcgagccgga agcgcgcgag gtgatctgct ccttgaggtt
     6181 gccgatctcc tcgctctcct cctcgatctc ggcgatgacg cgttcgcgat cccagttgac
     6241 cttgaggccg aactcgacct tgccggccat cttggcgagc acgtccctga gcgcgtcgta
     6301 ggtgccgcgc agcagctcca gaatgtcctc ctcggtgcgg aacaggacgc cgaaggccat
     6361 cgggagcacg gtgaactcgc gcatcacggt ctcgttgacc tgctcgtgcg cgaagacgtt
     6421 ctcgcgcgtc gggtcgtaga ccatgatcgg cgtgttcgag acgaccgccg cgaggccgtc
     6481 gtagtggacg gtgtacacgt cgtcgccgcg cccgccgatg ccgtgggggc cgaaactgcg
     6541 cggctccttc gactcgatga tgcagtagac gtacttgccc tccggcggag gggtcgcggc
     6601 ggcggcggaa atctggtcgg tttcagcact cataagcttt cggggggttc tgctccgtga
     6661 cttctgtctc tggggcggtg ttcgagcgac cacgagtata ataagattga gcgcacgggc
     6721 cacgcaacta ttatgcgccg aagcccccgg cgggttagaa ttaaccaact gaacccgacg
     6781 gggaagagaa gcgtaataca gttttcagtt ttcagtcttc agttttcaga aa
//
LOCUS       DBNGOH010000779         2973 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4148942, whole genome shotgun sequence.
ACCESSION   DBNGOH010000779 DBNGOH010000000
VERSION     DBNGOH010000779.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2973)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2973)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2973
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4148942"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(187..1983)
                     /locus_tag="AC159P_14990"
     CDS             complement(187..1983)
                     /locus_tag="AC159P_14990"
                     /inference="COORDINATES: protein motif:HMM:NF025006.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MSCRAMM family protein"
                     /protein_id="HIR3696805.1"
                     /translation="MRKTKAMPNACARATFVLAALLLLLPCAAPSHAQSEQSKAAPAT
                     VTGRVTDGKRGLAGINIVVTSMEVGMFSPATASAKTDAEGRYRLNGLAPGRYNVAPVA
                     PAHVMPEEVISFIGRQQGRSLVLSPGENIENVDFRLARGGVITGRVTDADGKPVVGEF
                     VQIAPEEGNRPAHQFFRPDRGEMQTDDRGVYRVYGLPAGRYRVSVGWTGPPRGPRNTV
                     ASGEHTFYERTYYPGTTDLAQAKIVEVSEGGEATDIDISVSKTTRQTYSAAGRLVFAE
                     TGQPAANVRVGYGAQGRDGRVMGAAFGGLGGGPNSVVTDARGEFKLEGLASGRYAAFG
                     VREQGSDWYSDQSAFEIAEANFEGIEVKLRRGSSLSGVVLIEGVSDRAAAQRLLSRAR
                     VFVMPAAPGGPPMPGAVVSETVAPDGSFRVGGLQPGRVRLGFSAERGLNLSMSRVEHN
                     GVPLGREGSVEVAEGAQVTGVRVIVAHGSASVRGKINIVGGTLAPGYSLRVFARRVGD
                     ENQFGGRAVEADSRGSFVMEGVAPGDYEFYAAAFFLVFQANASPPGPRSYNGPRQRFS
                     VAQDGDHNITLVLNVGDGNTPNVRPVSPPNTP"
     gene            2359..>2973
                     /locus_tag="AC159P_14995"
     CDS             2359..>2973
                     /locus_tag="AC159P_14995"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: protein motif:HMM:NF014896.7"
                     /note="PtrB; oligopeptidase that cleaves peptide bonds
                     following arginine and lysine residues; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HIR3696806.1"
                     /translation="MKRLSLRLALPALCLSLLNLNLIAQTNNAMTTGTLPPPPAAKKT
                     PKITEIHGEKLSDDYFWMREKTNPDVTAHLEAENAYTDAVMKPTADLQARLYKEMLGR
                     IKQTDTQVPYRDNGYLYYSRTVEGQQYPIYARRKGSMTAPEEVTLDLNELNKGHNYTA
                     MATYAVSDDSHLLAYSIDHNGYREYILKIKDLRTGQDLADEFGKV"
BASE COUNT          477 a         1055 c         1004 g          437 t
ORIGIN      
        1 ccgtgatcga gccggcgccg cccttcgtcg ctttcgcgtc ggggcggctc gtcgtctcgg
       61 gcgtcggcgc gggactgggc tgagaggcgg cgggcgactc ctgcgcgcgc gccggcggcg
      121 cgagcgcgaa gacgagcgcg aagacgaggg cggcgatgtt ggacgaggtg ctcaagggtt
      181 tctgcctcag ggcgtgttcg gagggctgac ggggcggacg ttcggcgtgt tgccgtcgcc
      241 cacgttgagg acgagcgtga tgttgtggtc gccgtcctgc gcgaccgaga agcgttgccg
      301 cgggccgttg tagctgcgcg ggccaggggg cgacgcgttc gcttgaaaga cgaggaagaa
      361 cgcggcggcg taaaactcgt agtcgccggg cgccacgcct tccatgacga aggagccgcg
      421 cgagtcggcc tcgaccgccc gcccgccgaa ctgattctcg tcgccgacgc gccgcgcgaa
      481 gacgcgcagc gagtagcccg gcgcgagcgt cccgccgacg atgttgatct tcccgcgcac
      541 ggacgcgctg ccgtgggcga cgatgacgcg cacgcccgtc acctgcgcgc cttccgcgac
      601 ctcgacgctg ccctcgcgcc cgaggggcac gccgttgtgc tcgacgcgcg acatcgagag
      661 gttgaggccc cgctcggcgg agaagccgag gcggacgcgg cccggttgca gcccgccgac
      721 gcggaagctg ccgtccgggg cgaccgtctc gctgacgacg gcgcccggca tcggggggcc
      781 gcccggcgcc gcgggcatga cgaagacgcg tgcgcgactc aacagcctct gcgccgccgc
      841 gcggtcgctc acgccctcga tgaggacgac gccgctcaaa gaagagccgc gcctgagctt
      901 gacctcgatg ccttcgaagt tcgcctccgc gatctcgaac gcggactggt cgctgtacca
      961 gtcggagcct tgctccctca cgccgaacgc ggcgtagcgt cccgacgcca gaccttccag
     1021 cttgaactcg ccgcgcgcgt ccgtcacgac cgagttcggg ccgcccccta acccgccgaa
     1081 cgccgcgccc atgaccctgc cgtcgcgccc ctgcgcgccg tagccgacgc ggacgttcgc
     1141 cgcgggctgt cccgtctcgg cgaagaccag ccggcccgcc gcgctgtagg tctgccgcgt
     1201 cgtcttcgac acggagatgt ctatgtccgt ggcttcgccg ccctccgaga cctcgacgat
     1261 cttggcctgc gccaagtcgg tggtgccggg gtagtaggtg cgctcgtaaa aggtgtgctc
     1321 gccgctggcg acggtgttgc gcgggccgcg cggcgggccg gtccagccga cgctgacgcg
     1381 gtagcgaccc gcgggcaggc cgtagacgcg gtagacgccg cggtcgtccg tctgcatctc
     1441 gccgcggtcg ggccggaaga actggtgcgc cgggcggttg ccctcttcgg gcgcgatctg
     1501 cacgaactcg ccgacgaccg gcttgccgtc ggcgtccgtc acgcgccccg tgatgacgcc
     1561 gccgcgcgcg aggcggaagt caacgttctc gatgttctcg ccggggctga ggacgagcga
     1621 gcgcccctgc tggcgcccga tgaagctgat aacctcttcc ggcatgacgt gggccggggc
     1681 gaccggcgcg acgttgtagc ggcccggcgc gagaccgttc aggcggtagc gcccttcggc
     1741 gtcggtcttc gcgctcgcgg tggcgggcga gaacatcccc acctccatgg acgtgacgac
     1801 gatgttgatg cccgccagcc cgcgcttgcc gtccgtcacg cgcccggtga cggtcgcggg
     1861 cgccgccttc gactgttccg actgcgcgtg agacggcgcg gcgcacggca gcagcagcag
     1921 cagcgccgcg aggacgaacg tggcgcgcgc gcacgcgttc ggcatggctt ttgtctttct
     1981 catgattcac ctgagcggca gacggcgggt gccggtcggc tgcctccgaa tttaaacgcg
     2041 gcgcgagcgc gccggttcga ctttctctcg acgcccgcca gcgtacaacc cgcgccgcgc
     2101 gccgcgcaag ccacaggcgg aaaggggaaa gggggaaaag gggaagaggc gaaaagggga
     2161 aaagtgtatc ctcccctttt cgcctcttcc ccttttcccc ctttcccctg tggcttgcag
     2221 cccttccgag cggcgcacta taatccggcg catcacggcc ttctcccgcg cgcgctgact
     2281 gtgagggccg cccttcgtac cgcaaacggc gacgacatca agacattcgc tccaaggaag
     2341 acacgcacgg aggttgcgat gaaaagatta tccctgcggc tggcgctgcc cgccctctgc
     2401 ctctccctct tgaacctgaa cctgattgca cagaccaaca acgcgatgac gacaggcacg
     2461 ctcccgccgc cgcccgcggc gaagaagaca ccgaagatca ccgagattca cggcgagaag
     2521 ctctcggacg actacttctg gatgcgcgag aagaccaacc cggacgtgac cgcgcacctc
     2581 gaagccgaga acgcctacac cgacgccgtg atgaagccga ccgcggacct tcaggcccgg
     2641 ctctacaaag agatgctcgg tcgcatcaag cagaccgaca cgcaggtgcc ctaccgcgac
     2701 aacggctacc tctactactc gcgcaccgtc gagggccagc agtacccgat ctacgcgcgc
     2761 cgcaaaggct cgatgaccgc gcccgaggag gtcacgctcg acctcaacga gctgaacaag
     2821 gggcacaact acaccgcgat ggcgacctac gccgtctcgg acgactcgca cctgctggcc
     2881 tactccatcg accacaacgg ctaccgcgag tacatcctca agatcaaaga cctgcgcacg
     2941 ggtcaggacc tcgcggacga gttcggcaag gtc
//
LOCUS       DBNGOH010000780         3735 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2316497, whole genome shotgun sequence.
ACCESSION   DBNGOH010000780 DBNGOH010000000
VERSION     DBNGOH010000780.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3735)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3735)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3735
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2316497"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1107)
                     /locus_tag="AC159P_15000"
     CDS             complement(<1..1107)
                     /locus_tag="AC159P_15000"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002613195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HIR3696807.1"
                     /translation="MPTTTPERAARARPLEQGHELVERAYLRATGAPLVAGNRVRLLR
                     DAAENYPAWLDSIKGAQRTIHFESYIIHEDEAGREFAAALAAKAREGVRVRIIYDWMG
                     AFGKTSNRFWRGLKEAGAEVRCYNPPSFFSPFGWVSRDHRKMIAVDGRVGYVTGLCVG
                     QDWIGRPEKKQEPWRDTGVEVRGPAVADIERAFAQVWAATGAPLPEEEMPEREEIEEQ
                     GETSLRVVAGEPQSAEMYRLDQIVAAGARRTLWLTDAYFVGTTTYVQALRAAARDGVD
                     VRLLVPSSSDIPVVNALSRANYRALLEGGVRVFEWNGSMLHAKTAVADGRWARVGSTN
                     LNVASWIGNYELDVAVEDERFAREMEEMYEQDLGH"
     gene            1310..2002
                     /gene="rpiA"
                     /locus_tag="AC159P_15005"
     CDS             1310..2002
                     /gene="rpiA"
                     /locus_tag="AC159P_15005"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015844279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HIR3696808.1"
                     /translation="MSEPLNAKQLAGARAAEYVEDGMAVGLGTGSTAYWMVEALGVRV
                     KHGLRVRGVPTSRRTEEQARALGIPLVTFAEVQQLDLTIDGADEIDPSLALTKGGGGA
                     LLREKLVAAASGRMIVVADSSKLVPVLGKFPLPVEVVPFAWEVTARRVEALGCNPRLR
                     HAGDGEDAYVTDNGNYILDCDCGVIADPARLERELKLLPGVVECGLFVGMAETVVVAD
                     EAGGVRLIERAG"
     gene            2009..2632
                     /locus_tag="AC159P_15010"
     CDS             2009..2632
                     /locus_tag="AC159P_15010"
                     /inference="COORDINATES: protein motif:HMM:NF020893.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HIR3696809.1"
                     /translation="MRPALTLLFAASAARRIIAFFVRLGLFGPFLLEALSTSFFYVPL
                     ANEFLLFALVSREGGGAWWPVYALMAAAGAVAGAFVLDLVVRRAGEKGAERFVRPRTY
                     ARLKSKLEAHAGRVLFVAAALPPPFPFRVTLMAASALQTPRARLLTAIFFGRALRFTA
                     ESLLILYLGRRFLKYMNSDAVEYAVYAFIVVALVGSVFTIYKLFGRR"
     gene            complement(2697..>3735)
                     /locus_tag="AC159P_15015"
     CDS             complement(2697..>3735)
                     /locus_tag="AC159P_15015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HIR3696810.1"
                     /translation="GYGPGRDYGADAYGGRVEERDYVRDYGRDYDQDYGRGYGRGFER
                     DDDARYGRERYYGESTGTRGGRGYGFGYARDYGGDYGRGYDRGYESGYEGEDDRDYGR
                     DAERDRARGTQTDYGRTSSRFFGRGGGEYEGRYARGYDEDDDEERGARRYDDDDERRE
                     GRGGRGWLDRVSDEVASWFGDREAVRRRRLDEKRARRRGRGPKGYRRSDARIGEDIND
                     RLSDQDYLDASEVVVVVNGGEVTLDGTVETRYDKRLAEDIAESVYGVTHVQNNLRVEY
                     RPAPARADETTIVASRRPATTTNAPTIETATETPTPTPGAATGATTGATTNVPDTGGM
                     SSSAASGTGRG"
BASE COUNT          480 a         1397 c         1229 g          629 t
ORIGIN      
        1 gtgtccgagg tcttgctcgt acatctcttc catctcgcgc gcgaaccgct cgtcctcgac
       61 cgccacgtcg agttcgtagt tgccgatcca gctcgccacg ttgaggttcg tcgagccgac
      121 gcgcgcccaa cgcccgtccg ccaccgccgt cttcgcgtgg agcatcgagc cgttccactc
      181 gaagacgcgc acgccgcctt ccagcagcgc gcggtagttg gcccgagaga gagcgttgac
      241 gacggggatg tcgctcgaac tcgggacgag caggcgcacg tccacgccgt cgcgcgccgc
      301 cgcgcgcaaa gcctgcacgt aggtcgtggt gccgacgaag taggcgtccg tcagccacag
      361 cgtgcggcgc gcgcccgcgg cgacgatctg gtcgagccgg tacatctccg ccgactgcgg
      421 ctcgccggcg acgacgcgca gagaggtctc gccctgctct tcgatctcct cgcgttcggg
      481 catctcttct tcgggcagag gggcgccggt cgctgcccac acctgcgcga aggcgcgctc
      541 gatgtcggcg accgcagggc cgcgcacctc gacgcccgtg tcgcgccacg gctcctgctt
      601 cttctcgggc cgcccgatcc agtcctgccc gacgcacagg cccgtcacgt agccgacgcg
      661 cccgtccacc gcgatcatct tccggtggtc gcggctcacc caaccgaagg ggctgaagaa
      721 gctcggcggg ttgtagcagc gcacctctgc gcccgcctcc ttcagcccgc gccagaagcg
      781 gttcgaagtc ttgccgaacg cgcccatcca gtcgtagatg atgcggacgc ggacgccctc
      841 gcgcgccttc gccgccagcg ccgcggcgaa ctcgcgcccc gcctcgtcct cgtggatgat
      901 gtagctctcg aagtggatgg tgcgctgcgc gcccttgatg gagtcgagcc acgcggggta
      961 gttctccgcg gcgtcgcgca gcaggcgcac gcggttgccc gcgacgagcg gcgcgcccgt
     1021 cgcgcgcagg tacgcgcgct cgaccagctc gtgcccctgc tccaaaggcc gcgcgcgcgc
     1081 ggctctctcc ggtgtagttg tcggcatgaa gggtcggccc tctaaaaagt tctagcgaca
     1141 cgggcacgga ggcggcggga tggaagtggc ttcgtcgtgg cgggggagcc gaacccgtcg
     1201 gctcccccgc cacgtcgctc gtctttcctc ttcatgtccc tacacctgcg gtgccggcgg
     1261 cggcaatttc atcttgcgcg agagagtcgt cgaaaacata atagccgaaa tgagcgaacc
     1321 ccttaacgcg aaacagctgg ccggcgcgcg cgccgccgaa tatgtagagg acggcatggc
     1381 cgtcggactc ggcaccggct cgaccgccta ctggatggtc gaggctctgg gcgtgcgcgt
     1441 caaacatggc ttgcgcgtgc gcggcgtgcc gacctccagg cgcacggagg agcaggcccg
     1501 cgcgctcggc atcccgctcg tcaccttcgc cgaagtgcag cagctcgacc tgaccatcga
     1561 cggcgccgac gagatcgacc cgagcctcgc gctcaccaaa ggcggcggcg gcgcgctcct
     1621 gcgcgagaag ctggtcgcgg cggcctcggg ccgcatgatc gtcgtcgccg actcgtcgaa
     1681 gctcgtcccc gtgctcggca agttccctct gcccgtcgag gtcgtgccct tcgcgtggga
     1741 ggtgacggcg cgccgcgtcg aagccctcgg ctgcaacccg cggctcaggc acgcgggcga
     1801 cggcgaagac gcttacgtca ccgacaacgg caactacatc ctcgactgcg actgcggcgt
     1861 catcgcagac ccggcgcggc tcgaacgcga gctgaagctg ttgccgggcg tggtcgagtg
     1921 cggactcttc gtcgggatgg ccgagacggt cgtcgtggcc gacgaagcgg gcggcgtccg
     1981 cctcatcgaa agggccggtt aagccgcggt gcgtcccgcc ctcacccttc tcttcgcggc
     2041 ctcggcggcg cgccggatca tcgccttctt cgtgcggctc gggctgttcg gcccgttcct
     2101 gctcgaagcg ctctccacct ccttcttcta cgtgccgctg gcgaacgagt ttctgctctt
     2161 cgcgctcgtc agccgcgagg gcggcggcgc gtggtggccc gtctacgcct tgatggccgc
     2221 cgccggcgcg gtcgcgggcg ccttcgtctt agacctcgtc gtgcgccgcg cgggcgagaa
     2281 gggcgccgag cggttcgtgc gcccgcgcac gtatgcgcgc ctcaagtcga agctcgaagc
     2341 gcacgcgggc cgcgtcctct tcgtcgccgc cgcgctcccg ccgcccttcc ctttccgcgt
     2401 cacgctgatg gcggcctcgg cgctccagac cccgcgcgcg cgcctgctca cggccatctt
     2461 cttcgggcgc gcgctgcgct tcacggcgga gtctctgctg atcctctacc tgggccgccg
     2521 cttcctcaag tacatgaact ccgacgcggt cgagtacgcc gtctacgcct tcatcgtcgt
     2581 cgcgctcgtc ggcagcgtct tcaccatcta caagctgttc ggcagacgtt gaagacgcac
     2641 cgacgaacga cgttcatcaa cgaacgttac ggcgcgacga gccgcgccgc gcgctctcaa
     2701 ccacggcccg tgcccgacgc ggcactcgaa ctcatgcctc cggtgtccgg cacattggtc
     2761 gtcgcgccgg tcgtcgcgcc ggtcgccgcg ccgggggtgg gcgtcggggt ctcggtcgcc
     2821 gtctctatcg tcggtgcgtt ggtcgtcgtc gcgggacggc ggctcgccac gatggtcgtc
     2881 tcgtcggcgc gcgcgggggc ggggcgatac tccacgcgca ggttgttctg gacgtgcgtg
     2941 acgccgtaga cggactccgc gatgtcttcg gcgaggcgct tgtcgtagcg cgtctcgacc
     3001 gtgccgtcga gcgtgacctc gccgccgttc acgaccacca cgacctccga ggcgtcgagg
     3061 tagtcctgat cggacagccg gtcgttgatg tcctcgccga tgcgcgcgtc cgagcggcgg
     3121 tagcctttcg gcccgcgccc gcgccggcgc gcgcgcttct cgtccagacg ccgccgccgc
     3181 acggcctccc tgtcgccgaa ccaggaggcc acctcgtccg acacgcgatc cagccagccg
     3241 cgcccgccgc gcccttcacg acgttcgtcg tcgtcgtcgt aacgacgcgc gccgcgctcc
     3301 tcgtcgtcgt cttcatcgta gccgcgcgcg tagcggccct cgtattcgcc gccgccgcga
     3361 ccgaagaagc gactcgaagt gcgcccgtag tcggtctgcg tgccgcgcgc gcggtcacgc
     3421 tctgcgtcgc gtccgtaatc gcggtcgtcc tcgccttcgt agccgctctc gtagccgcgg
     3481 tcataaccgc gcccgtagtc gcccccgtaa tcacgcgcgt aaccgaaacc gtagccgcgc
     3541 ccgccccgcg tgccggtcga ttcgccgtag tatctctcgc gcccgtagcg cgcgtcgtcg
     3601 tcgcgctcga agccgcgccc gtagccgcgt ccgtagtcct ggtcgtagtc gcgaccgtaa
     3661 tcacgaacgt agtcgcgctc ttcgacgcgc ccgccgtacg cgtcggcgcc gtagtcgcgg
     3721 cccggcccgt agccc
//
LOCUS       DBNGOH010000781         7523 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_667250, whole genome shotgun sequence.
ACCESSION   DBNGOH010000781 DBNGOH010000000
VERSION     DBNGOH010000781.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7523)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7523)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7523
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_667250"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            229..1605
                     /gene="mgtE"
                     /locus_tag="AC159P_15020"
     CDS             229..1605
                     /gene="mgtE"
                     /locus_tag="AC159P_15020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HIR3696811.1"
                     /translation="MVAELQPDIERMIREREFFALRRELAGWPPPDLARLISELETDE
                     QVIVFRILPRKLATDVFEYLDLEVQEQLLKTMAQTDVTRILNDMAPDDRTELLEELPA
                     AITRQMLALLTPDERAVAVSLLGYPEGSIGRLMTPDYVRVKREWTVQHALDHIRRHGR
                     DSETLSIIYVVDERGALIDDIRIREFLLAEPESLISELMDERFVALKATDDQEAAVAV
                     FRDEDRSALPVTDSTGVLIGIVTFDDVLDVAEEEATEDIQKFGGTEALEDPYMQTTFA
                     SMIRKRAGWLVLLFFGEMLTATAMGYFEDEIQRAVVLALFVPLIISSGGNSGSQAATL
                     VIRALALGEVTLRDWWRVMRREILSGLTLGAILGLIGFLRIELWSQFSDFYGPHATEV
                     ALTVGFTLVGIVMWGTLAGSMLPFALRRLGFDPATSSAPFIATLVDVTGLVIYFTVAA
                     LILRGTLL"
     gene            1722..3158
                     /locus_tag="AC159P_15025"
     CDS             1722..3158
                     /locus_tag="AC159P_15025"
                     /inference="COORDINATES: protein motif:HMM:NF012257.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HIR3696812.1"
                     /translation="MKRPDLRKQLSLPVLFVALFGLAFYVLVYFAPEPPPDAASDERR
                     MVFAAYEWARALVSIALWISAAFVAVRALNELIFFVFRKRKGYEAPSLMRDIFSLAAY
                     VTAVALILKLHFANLSFGALLSGSALLGIILGLALQDTLGNLFAGISLQADKPFQVGD
                     VIAVGAATGVVESVTWRAVKIKTFQNHIILLGNSQIAKEHIEVCPRGNANARIVFFNA
                     VYTDSPTKVIHVVREAVRDCENVLRYMTPVVRIRGMGADGVDYEVKYWLSDYARYNDT
                     DALVRQRIWYAFRRNGLTFAYPTRTVYFQRAVSADGVGHETLTERLSAIDIFSPLTDE
                     ELRALAENSSGHVYAPGELIIRAGDQGASMFVIHRGSVDVKIDTNGEARTINTLREGD
                     FFGEMALFTGEPRTANVIAAEETEVLEIGKRAMQDLFENNADLAESLSHTINERRAML
                     AARTAAPSSEEDSPAGLLFKIKRFFRLD"
     gene            complement(3330..4313)
                     /locus_tag="AC159P_15030"
     CDS             complement(3330..4313)
                     /locus_tag="AC159P_15030"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HIR3696813.1"
                     /translation="MANREKWAAYGAWAAVCFFWGTTYLAIRVGLETLTPMLFAGLRF
                     LIAGGVLFFVMGRQRNARLPKGREWLHLAFVGLLLLGVGNGAVVWAEQWVPSGMASLL
                     VATSPFWVVALERFRKDGERVGVRGVVGMLVGFAGLGLLLAPQLFDASVSAYLLLGAL
                     AIQIGCFTWTVGSMYAKHRPVGVAPLMGAAVQMLIAGAVLTLVGTFLGEWSEISFSAR
                     SAGALAYLVVFGSIVAYSAYTYALQKLPLSLVSTYSYINPVIAVLLGWLVLAEPLDWR
                     VGAATAIILLGVALLKTSPKELAALWARLRAWRERRAAARAADDSLGVACE"
     gene            complement(4313..4792)
                     /locus_tag="AC159P_15035"
     CDS             complement(4313..4792)
                     /locus_tag="AC159P_15035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HIR3696814.1"
                     /translation="MDEKDARILEILQRDGRVTNVELARAVELTPSATLERVRKLEER
                     GLIRGYTAVLDPQALGLGLMAFIFVRVDDRDDIVPKAEGTAEALAELPSVQELHHLAG
                     EDCFLLKVRAKDTDDLYRILRDELGQFKGIRGTRTTIVLKTVKESAVLPIKRESEGG"
     gene            5051..5590
                     /locus_tag="AC159P_15040"
     CDS             5051..5590
                     /locus_tag="AC159P_15040"
                     /inference="COORDINATES: protein motif:HMM:NF012241.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HIR3696815.1"
                     /translation="MSITRYDPFRDLRTLQDEVNRLFSSNFSRSFGEEGFARGAWAPS
                     VDIYENKDQIVLEAELPGMTREDFELTVENNVLTLRGERRFEKKEESDNYHRVERAYG
                     SFSRSFTLPQTVSSENAIAEYKNGVLRVVLQKREEVKARRIEIGGESADANTPRTIEA
                     KAEQTGETGGESKTKAANR"
     gene            5745..>7523
                     /locus_tag="AC159P_15045"
     CDS             5745..>7523
                     /locus_tag="AC159P_15045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HIR3696816.1"
                     /translation="MRLDRFTLRGQEAIQSAIEAAEKNQNQQVEPEHVLLAMLEQPEG
                     VVRPILGKLGANVQVVLNDVQAAVARFPKVQGGQQYFSPRLTQVFNASQKHAERMQDE
                     YISTEHLLLAISDEKDGAAGRVLRQHGVNSADLAKVIEQMRGGARVTDQNAEANYQAL
                     TKYAKDLTELARQGKLDPVIGRDDEIRRTVQVLSRRTKNNPVLIGEPGVGKTAIVEGL
                     AQRIVSGDVPETLRNKRLVSLDLGSMLAGAKYRGEFEDRLKAVLKEIENSQGHIVLFI
                     DELHTLVGAGAAEGAIDASNMLKPALARGELRCVGATTLNEYKKHIEKDAALERRFQP
                     VVVGEPTVEDTIAILRGLKERYEVHHGVRIKDSAIVAAATLSNRYITDRFLPDKAIDL
                     IDEAASRLRIEIDSLPAEIDVLEREILQLEIERQALQREEDERSKSRLKDIEQRIADL
                     NEKSSGKKAKWQSEKEEIERMREDKNELEQLRLQLDQARNAGDLTRAAEIQYGRIPEL
                     EQKFAAEREKLAELQRDGVMLKEEVDEEDVAQVVAKWTGVPVSKMLEGEMQKLVTMED
                     RLSQRVIGQEEALTAVANAVRRARAGL"
BASE COUNT         1437 a         2608 c         2278 g         1200 t
ORIGIN      
        1 ctgaggactg aggactgagg acggaggtaa aacgttaatg aggtgaagca gaagaccgag
       61 ctaagaagat gatgctcttc ctcagtcctc ggtcctcagt cctttggttc gtgctatgat
      121 gccgtcctgc tttcggggct tttaagtttg ccgtcaaaat acgttccgtg agagacggtg
      181 cgcgccgtgg cctgccgggg cggcgtgtgc ttcgaggcga gtcccgcgat ggtggccgag
      241 ctgcaaccgg atatcgagcg gatgatccgc gagcgcgagt tcttcgcgct ccggcgcgag
      301 ctggccggct ggccgccgcc cgacctcgcg cgcctcatct ccgagctgga gaccgacgag
      361 caggtcatcg tcttccgcat cctcccgcgc aagctcgcca cggacgtctt cgaatacctc
      421 gacctcgaag tgcaggagca gctcctcaag acgatggcgc agacggacgt gacgcgcatc
      481 ctcaacgaca tggcgccgga cgaccgcacc gagctgctgg aagagctgcc cgccgccatc
      541 acccggcaga tgctcgcgct cctgacgccc gacgagcgcg ccgtcgccgt ctcgctcctc
      601 ggctacccgg agggcagcat cgggcggctg atgacgcccg actacgtgcg cgtcaagcgc
      661 gagtggacgg tgcagcacgc gctcgaccac atccgccgcc acgggcgcga cagcgagacg
      721 ctctcgatca tctacgtcgt ggacgagcgc ggcgcgctca tcgacgacat ccgcatccgc
      781 gagttcctgc tcgccgaacc cgagagcctc atctccgagc tgatggacga gcgcttcgtc
      841 gcgctcaagg cgaccgacga ccaggaggcc gccgtcgccg tcttccgcga cgaagaccgc
      901 agcgcgctgc ccgtcaccga ctcgaccggc gtgctcatcg gcatcgtcac cttcgacgac
      961 gttctggacg tggccgagga ggaggcgacc gaggacatcc agaagttcgg cggcaccgag
     1021 gcgctcgaag acccctacat gcagaccacg ttcgcgagca tgatccgcaa gcgcgcgggc
     1081 tggctcgtcc tgctcttctt cggcgagatg ctgacggcga ccgcgatggg ctacttcgag
     1141 gacgagatac agcgcgccgt cgtgctcgcg ctcttcgtcc cgctcatcat ctcctcgggc
     1201 ggcaactccg gctcgcaggc cgcgacgctc gtcatccgcg cgctcgcgct cggcgaagtc
     1261 accctgcgcg actggtggcg cgtcatgcgg cgcgagattc tgtcgggcct cacgctcggc
     1321 gcgatcctcg gcctcatcgg cttcctgcgc atcgagctgt ggtcgcagtt ctccgacttc
     1381 tacggcccgc acgcgaccga ggtcgcgctc accgtcggct tcacgctcgt cggcatcgtc
     1441 atgtggggca cgctcgccgg ctcgatgctc ccgttcgcgc tgcgccgcct cggcttcgac
     1501 ccggccacct cctccgcccc cttcatcgcc acgctcgtcg atgtgaccgg cctcgtcatc
     1561 tacttcaccg tcgccgcgct catcctgcgc ggcacgctcc tttagtgaaa gttcgcggcc
     1621 cgaagtcggg aagcctctgg taaactttgg gcctccgacc gcgagccgcg ggctgaccgc
     1681 tcggacttcg gacttcgaac ttcggacatt gagctgacgc cgtgaaacgc cccgacctga
     1741 gaaaacaact ttccctcccg gtgctcttcg tcgccctctt cgggctggcc ttctacgtcc
     1801 tcgtctactt cgcgcccgag ccgccgcccg acgccgcgtc cgacgagcgg cggatggtgt
     1861 tcgccgccta cgagtgggcg cgcgcgctcg tcagcatcgc gctctggatc agcgccgcct
     1921 tcgtcgccgt gcgcgcgctg aacgagctga tcttcttcgt cttccgcaag cgcaagggct
     1981 acgaggcgcc gagcctgatg cgcgacatct tctcgctcgc cgcctacgtc acggcggtcg
     2041 ccctcatcct caagctccac ttcgccaacc tctcgttcgg cgcgctgctc tccggctcgg
     2101 cgctcctcgg catcatcctc ggcctcgcct tgcaggacac gctcggcaac ctcttcgcgg
     2161 gcatctcgct ccaggccgac aagcccttcc aggtcggcga cgtgatcgcg gtcggcgccg
     2221 cgaccggcgt cgtcgagagc gtgacgtggc gcgccgtcaa gatcaagacc ttccagaacc
     2281 acatcatcct gctcggcaac agccagatcg ccaaagagca catcgaggtc tgcccgcgcg
     2341 gcaacgccaa cgcccgcatc gtcttcttca acgccgtcta cacggactcg ccgaccaagg
     2401 tcatccacgt cgtccgcgag gccgtgcgcg actgcgagaa cgtgctgcgt tacatgacgc
     2461 ccgtcgtccg catccgcggc atgggggccg acggcgtgga ctacgaggtc aagtactggc
     2521 tctccgacta cgcgcgctac aacgacacgg acgcgctcgt ccgccagcgc atctggtacg
     2581 ccttccgccg caacgggctg acgttcgcct acccgacgcg caccgtctac ttccagcgcg
     2641 ccgtctctgc cgacggcgtc gggcacgaga cgctgaccga gcgcctctcg gccatcgaca
     2701 tcttctcgcc gctcacggac gaagagctgc gggcgctcgc cgagaactcc agcggccacg
     2761 tctacgcgcc cggcgaactc atcatccgcg ccggggatca gggcgcctcg atgttcgtca
     2821 tccaccgcgg cagcgtggac gtgaagatcg acaccaacgg cgaggcgcgc accatcaaca
     2881 cgctgcgcga gggcgacttc ttcggcgaga tggcgctctt cacgggcgag ccgcgcaccg
     2941 ccaacgtcat cgccgccgaa gagacggagg tgctggagat cggcaagcgg gcgatgcaag
     3001 acctcttcga aaacaacgcc gacctcgccg aatcgctcag ccacaccatc aacgagcgcc
     3061 gcgccatgct cgccgcccgc accgcggccc cctccagcga agaggactcg cccgccggcc
     3121 tcctcttcaa gatcaaacgc ttcttccgcc tcgactaaag aagaagaggg gaaaaggcga
     3181 aaaggcgaaa aggaaggacg aaaagcagcg tgcctctcat caacttgatg agaggcacgc
     3241 tgcttttcat gttcggttga ggatgagaag cagcgtgctt ctcatcctcc ctcttcgcct
     3301 tttaccctct gccccttttc ccctctgttt tattcacagg ccacgccgag cgaatcgtcc
     3361 gcggcccgcg ccgcggcgcg ccgctccctc cacgcccgca gccgcgccca cagcgccgcc
     3421 aactccttcg gcgaagtctt gagcagcgcc acgcccaaaa ggatgatcgc cgtcgccgcg
     3481 cccacgcgcc agtcgagcgg ctcggccagc acgagccagc cgagcagcac cgcgatcacc
     3541 gggttgatgt acgagtaggt cgagacgagc gacagcggca gcttttgcag cgcgtacgtg
     3601 taagcgctgt aggccacgat cgagccgaac acgacgaggt acgcgagcgc ccccgcgctc
     3661 cgcgcgctga aactgatctc gctccactcg cccaggaacg tgccgaccag cgtcagcacc
     3721 gcgcccgcga tcagcatctg caccgctgcc cccatcaacg gcgccacccc caccggcctg
     3781 tgcttcgcgt acatagaccc gacagtccac gtaaaacacc cgatctgaat cgccagcgcg
     3841 ccgagcaaca ggtaggcgct caccgaagcg tcgaagagtt gcggcgccag cagcagcccc
     3901 agccccgcga acccgaccag catcccgacg acgccgcgca cgcccacgcg ctcgccgtcc
     3961 ttcctgaacc gctccaacgc caccacccag aacggcgaag tggccaccag cagcgaagcc
     4021 atcccgctcg gcacccactg ctctgcccac accaccgcgc cgttcccgac gccgagcaac
     4081 agcagcccga cgaacgccag atgcagccac tcccgcccct tcggcagacg cgcgttccgc
     4141 tggcggccca tcacgaagaa gagcacgccg cccgcgatca gaaaccgcag ccccgcgaac
     4201 agcatcggcg tgagtgtctc aagcccgacg cggatcgcta agtaagtcgt cccccagaaa
     4261 aaacagaccg ccgcccaagc cccgtacgcc gcccacttct ccctgttcgc catcacccgc
     4321 cctcgctttc cctcttgatc ggcaacaccg ccgactcctt caccgtcttg agcacaatcg
     4381 tcgtccgcgt cccgcggatg cctttgaact gccccagctc gtcacgcagg atgcggtaca
     4441 aatcatccgt gtccttcgcc ctcaccttca gcaggaagca gtcctcgccc gccaggtggt
     4501 gtagctcctg caccgacggc aactctgcca gcgcctccgc cgtcccttcg gctttcggca
     4561 cgatgtcgtc gcggtcgtcc acgcggacga agatgaaggc catcaggccg aggccgagcg
     4621 cctgcgggtc gagcaccgcc gtgtagcctc tgatcagccc gcgctcttcg agcttccgca
     4681 cgcgttcgag cgtcgccgaa ggcgtcaact cgacggcgcg ggccaactcg acgttggtca
     4741 cccgcccgtc gcgctgcaaa atttccaaaa tccgtgcgtc tttctcgtcc atcatgctct
     4801 tgacacttat ttcaatgatt ccgtcgtctt gaacgaaaat atacatctga aggttctatt
     4861 tgccaaatat aatttggcga cgagaggggg tcagcgaaga aaagcaacgt cacaatgcga
     4921 ttacaattga ctttagagga cttgacaacg tcccactcaa ggtttattat gctcctgcgc
     4981 tcaaacaccc aaaactttga cgggtagcca gaaaagcgca taattctctt tgaatttaag
     5041 gaggataacc atgagcatca ctcgctacga ccctttccgt gacctgcgga ctcttcagga
     5101 cgaggtcaac cgcctgttct ccagcaactt ctcgcgctcg ttcggcgaag agggcttcgc
     5161 gcgcggcgcc tgggcgccga gcgtggacat ctacgagaac aaagaccaga tcgtgctcga
     5221 agccgaactg cccggcatga cccgcgaaga cttcgagctg acggtcgaga acaacgtctt
     5281 gaccctgcgc ggcgagcgcc gcttcgagaa gaaggaagag tcggacaact accaccgcgt
     5341 cgagcgcgcc tacggcagct tctcgcgctc cttcacgctc ccgcagaccg tctcttccga
     5401 gaacgcgatc gccgagtaca agaacggcgt gctccgcgtc gtgctccaga agcgcgagga
     5461 agtgaaggcg cgtcgcatcg agatcggcgg cgagagcgcc gacgcgaaca cgccgcgcac
     5521 catcgaggcg aaggccgaac agacgggcga gaccggcggc gaatcgaaga cgaaagccgc
     5581 caatcgctaa accgaagtcg gaaggatgaa cgcggaacga tgaacgagga gtcaggagtc
     5641 ggaagtcagg agtcagaata aaaggtcttt ccttctgact tctgaattct gtcttccgac
     5701 ttcttatcgc cgttccgcgt tccacgttcg agcggtatcg aatcatgcgt ttagatcgtt
     5761 tcacactgcg cggccaggag gccatccagt cggccatcga ggccgccgag aagaatcaga
     5821 accagcaggt cgagccggag cacgtgctgc tcgcgatgct cgaacagccg gagggggtcg
     5881 tgcgcccgat cctgggcaag ctcggcgcga acgtgcaggt cgtcctgaac gacgtgcagg
     5941 cggcggtcgc gcgcttcccc aaagtgcagg gcgggcagca gtacttcagc ccgcggctga
     6001 cgcaggtttt caacgcctcg cagaagcacg ccgagcggat gcaggacgag tacatctcga
     6061 ccgagcacct gctgctcgcc atctcggacg agaaggacgg cgccgcggga cgcgtgctgc
     6121 gtcagcacgg cgtcaacagc gccgacctcg ccaaggtcat cgagcagatg cgcggcggcg
     6181 cgcgcgtcac cgaccagaac gccgaggcca actaccaggc gctcacgaag tacgcgaaag
     6241 acctgacgga gctggcgcgg cagggcaagc tcgaccccgt gatcgggcgc gacgacgaga
     6301 tacggcgcac cgtgcaggtg ctctcgcgcc ggacgaagaa caaccccgtg ctgatcggcg
     6361 agccgggcgt cggcaagacc gccatcgtcg agggtctcgc gcagcgcatc gtctcgggcg
     6421 acgtgccgga gaccttgcgc aacaagcgtc tcgtctcgct cgacctcggc tcgatgctcg
     6481 cgggcgcgaa gtaccgcggc gagttcgagg acagactcaa ggccgtcctc aaagagattg
     6541 aaaattcgca ggggcacatc gtgctcttca tcgacgagct gcacacgctg gtgggcgcgg
     6601 gcgcggctga aggggcgatt gacgcctcga acatgctgaa gccggcgctg gcgcgcggcg
     6661 agctgcggtg cgtcggcgcg acgaccttga acgagtacaa gaagcatatc gagaaggacg
     6721 cggcgctcga acggcgtttc cagcccgtcg tcgtcggcga gccgaccgtg gaggacacca
     6781 tcgccatcct gcgcggcttg aaggagcgct acgaggtgca ccacggcgtc cgcatcaagg
     6841 actcggccat cgtcgcggcg gcgacgctct cgaaccgcta catcacggac aggttcctgc
     6901 cggacaaggc gattgacctg atcgacgagg cggcctcgcg gctccgcatc gagatcgatt
     6961 cgctgcccgc ggagatcgac gtgctcgaac gcgaaatcct ccaactggag attgagcgtc
     7021 aagcgttgca gcgcgaagag gacgagcgct cgaagtcccg cctgaaggat atcgagcagc
     7081 gcatcgccga tctcaatgag aaatcgtcgg ggaagaaggc caagtggcag tccgagaagg
     7141 aagagatcga gcggatgcgc gaggacaaga atgagcttga acaactgcgc ttgcagttgg
     7201 atcaggcgcg caacgcgggc gaccttacgc gggctgccga gattcaatac ggaaggattc
     7261 ccgaactcga acaaaagttc gcggcggaga gagagaaact ggccgagctt cagcgcgacg
     7321 gcgtgatgct caaggaagaa gttgacgaag aggacgtggc gcaggtcgtc gcgaagtgga
     7381 cgggagtgcc ggtgtcgaag atgctcgaag gcgagatgca gaagctcgtc acgatggaag
     7441 accgcctgtc gcagcgcgtg atcgggcagg aggaggcgtt gacggcggtc gccaacgcgg
     7501 tgcgccgcgc gcgcgcgggg ctg
//
LOCUS       DBNGOH010000782         7619 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3783861, whole genome shotgun sequence.
ACCESSION   DBNGOH010000782 DBNGOH010000000
VERSION     DBNGOH010000782.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7619)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7619)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7619
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3783861"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            294..626
                     /locus_tag="AC159P_15050"
     CDS             294..626
                     /locus_tag="AC159P_15050"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HIR3696817.1"
                     /translation="MEDLASRHCVPCHGGVPRLGGDEVAELLKQLDEWEVVEEQHLSK
                     DYKFPNFAAALAFVNRVGAVAEAEGHHPDIFFGWGYAHVKIWTHAIDGLSESDFILAA
                     KIDKSREP"
     gene            745..1485
                     /locus_tag="AC159P_15055"
     CDS             745..1485
                     /locus_tag="AC159P_15055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696818.1"
                     /translation="MEIENILPRGRVEARGALCVECLSAVAPAGAVLDRTGAALCRAC
                     AAEFYTACAACAGLVPRDEALARDDVGGGDESKLYCAECFRAAAFAAEEAEPVSDEEV
                     TALVAEFVALHAEKKRLDARVEEIKERLKVAARARPRVAGAVVLRAGEDAGVRCSYSV
                     RTTYDAGKLEAVEAMLGGAEFAALFERKVTYSPVRNSLEEFLASSDDEHGAAREAIRA
                     AAQHTETATLNVVAQKKQKKAAKADEPQ"
     gene            1561..5766
                     /locus_tag="AC159P_15060"
     CDS             1561..5766
                     /locus_tag="AC159P_15060"
                     /inference="COORDINATES: protein motif:HMM:NF024991.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional RecB family nuclease/DEAD/DEAH box
                     helicase"
                     /protein_id="HIR3696819.1"
                     /translation="MTTRTPEPLSPFDTERPLTAQRLAQYVARGRCERHLRFALFKSE
                     AQSLMRRYGLEAEPLSPLLSGAGQRFEEERVAELAEGAEVRDLRGQSCENFIAAVKSQ
                     GAGRVFYYQPTLVGRIGGWACEGRADLIEVTREGARVEATVVDFKASARESVGFRLQV
                     AFYARLLAGALAAAGFEGFTVRGAVAARDSRQTPEGGWETFDLALYLDEIESLVAAPD
                     SDVARAARAVGGTARYHLRASCDGCPYNALCFADTAEREDLSLVPLLTPSEKRALEAE
                     GVTTARALAALMRYGAGAMEPAPGAEEEARRVSARWPLTGRLPTLVQRARAAVNRFDK
                     TVNTRRYLVGADYGSLPDEAQHPGLVRVFVDAQRDYLKDKLFLVAARVAGPRGTFELV
                     EMTDAPPDADTERAMLLAWVRRLLPAIADAADSQRAPLHFYTFAPRGERALLDALARH
                     FDALCAVPAFYDLLTSHPALSQPMVSLLSEEVRARLNLAPVCHNLYEVARALGFEWRD
                     RDTDFRQTFRARVFDNRRAFERDRATGRFKTEGKGGKGEEAKRLKGEEGKREDDKHAA
                     SHQVDDDRHAASHRQLDERHRASHQVDEKHGASHHPSSPFDLFPFSPSDDEDAPPPFV
                     YVESAARFGAEIPLEYAYAAWGALREPNEATGRERSQVKGFLGVSGEQLRAFAAHRTR
                     AMQHVEESFAYKNRRVEKEPLDLSRLHEVSVAPEEVPLHRSLEDFLLLEHHASFQERL
                     LHLSQPPRERAATGRAVVLRCESYEKDEERVERASLCFADAEGREQTVKDLGLLRLRA
                     GDWTALNPLADAEGRALPAWRLVRGRLAVVEELTDAGLSLRLMPMSFKNSPFRYPHGL
                     FAPTSGALYTLDEMADDLNADKFLEACRHAPTNHLYRWLEDAEEGRRPRALRPKRLRD
                     ALEVAALAARTQQPHGLTAAQTEIVGSHLEDRVLVVQGPPGTGKSHTLGFAVLARALA
                     QSTPARPFRVAVLARTHAAVNVALSSIAKRTRELLEVDEEHGASHQSSALQLAPLARL
                     RVVKVCNDAGEAVPEGVGRVLPGGDEEASAAEQWEQLLGEELLVVGGTPGGLYRLVRA
                     GAARGRKVNWAEKYFDLVVVDEASQMGLAEALTAAAFLRDDGQFIAVGDHRQMPPILA
                     HAWDREARRDIRRARPHLSVFEYLLEAGFARAALDESFRIPAEVADFLSRHVYAADGI
                     DFRSSNRKRLAAAEGIREDWLRAALAPEHALVVVEHDEAGSQQSNEFEASLVEAIARA
                     AAEQLSLDARKGLGVVVPHRAQKALLAARLPALADSIDTVERFQGGERDLIVVSATVS
                     DREFAAGESGFLLEPRRLTVAVSRPRRKLIVVASRAVFDLIPPDLDDYERGALWKHLR
                     REASARLLWQGEVAGHRVTVRAI"
     gene            6015..7466
                     /locus_tag="AC159P_15065"
     CDS             6015..7466
                     /locus_tag="AC159P_15065"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HIR3696820.1"
                     /translation="MAEKTRITVAHGDGIGPEIMGATLRILEGAGARLEIEEIEIGEK
                     VYLAGNPAGIAPEAFESLRRTKVFLKAPITTPQGGGYKSLNVTIRKTFGLYANVRPCV
                     SYHTFIETKHPVMDVVIVRENEEDVYGGIEYRQTKQVTQCLKLISRPGTEKIVRYAFE
                     YARANNRRKVTCFTKDNIMKITDGLFHKVFDEVAAGYTDIENEHWIVDIGSAKLADTP
                     EAFDVVVLPNLYGDVLSDVAAQIAGSVGLAGSANIGESIAMFEAIHGSAPRRAGQNVA
                     NPSGLLLGAVMMLVHIGQGDIAERVHNAWLRTVEEGIHTYDIYKEGVSREKVGTREFA
                     EAVVARMGQRPETLKAVSYKAGAPASLTGGKTSAASSETKALAGVDVYLDFSSGTSDE
                     LGAAMENLAGDGLRISAISNRGVKVWPGGNPETFCSDHWRCRFVADADGAAVTHAQVA
                     ALLKRAAEAGHDFIKTENLYNFDGKPGYSHAQE"
BASE COUNT         1260 a         2587 c         2596 g         1176 t
ORIGIN      
        1 cggaaacgaa ttgtgcggaa ctttaagccg ttagcttacc gactcgcccc gccgtttgcc
       61 aagtcgcggg gaaggggaag gggaaagagg ggaaaagggg aaagggcgaa aaaggaggat
      121 gagaagcacg ctgcctctcg tcaagtcgat gacgagaggc agcgtgcgtc tcatcctccc
      181 tcttcccctt ttcgcctctt gacctcttcg cccctcctgc tcccttttcg ccctttcccc
      241 ttttcccctc ttccccttct ccttttcccc tcttcccctt agaatcctgc ggcatggaag
      301 acctggcatc gcggcactgc gtgccgtgtc atggcggcgt gccgagactg ggcggcgacg
      361 aggtcgcgga gttgctgaag cagctcgacg agtgggaggt cgtcgaagag caacatctct
      421 cgaaggatta caagttcccg aacttcgccg cggcgctcgc gttcgtcaac cgcgtcggcg
      481 ccgtcgccga ggccgaaggc caccacccgg acatcttctt cggctggggc tacgcgcacg
      541 tcaaaatctg gacgcacgcc atcgacggcc tcagcgagag cgacttcatc ctcgccgcga
      601 aaatagataa aagccgtgag ccgtgaaccg taaaccgtga acggtgaaga gccggaacag
      661 catacctctc gtcactcgtc actcgtcacg gcttttcact tatcacggtt tacggttcac
      721 ggttcacggc ttttgaggtt gtcgatggaa atcgaaaaca tcttgccgcg ggggcgtgtg
      781 gaggcgcgtg gagcgctgtg cgtggagtgc ctgtcggcgg tggcgcccgc cggggccgtg
      841 ctcgaccgca cgggcgcggc gctgtgccgc gcgtgcgccg cggagttcta cacggcgtgc
      901 gccgcgtgcg cggggctggt gccgcgcgac gaggcgctgg cgcgcgacga cgtgggcggc
      961 ggcgacgaaa gtaagcttta ctgcgcggag tgcttccgcg cggcggcctt cgcggcggag
     1021 gaggccgagc cggtgagcga cgaggaggtg acggcgctgg tcgcggagtt cgtcgcgctc
     1081 cacgcggaga agaagcgtct ggacgcgcgc gtcgaagaga tcaaggagcg gctgaaggtg
     1141 gcggcgcgcg cgcgcccgcg cgtggccggg gcggtggtct tgcgcgcggg cgaggacgcg
     1201 ggcgtgcgtt gcagctacag cgtgcggacg acgtacgacg ccgggaagct cgaagcggtc
     1261 gaggccatgc tcggcggcgc ggagttcgcc gcgctcttcg agcgcaaggt cacgtacagc
     1321 cctgtcagga attctctcga agagttcctc gcgtcgtccg acgacgagca cggcgccgcg
     1381 cgcgaggcca tccgcgccgc cgcgcagcac acggagacgg cgacgctcaa cgtcgtcgcg
     1441 cagaagaagc agaagaaggc cgccaaggcg gacgagccgc agtaatcgca agtcaatcgc
     1501 agtgacctca tcgaacaacc ccaacgcgcg gcggcccgcg tccggtcgcc gcattctcgt
     1561 atgacgactc gaactcccga acccctctcg cccttcgaca cggagcggcc cctgacggcg
     1621 cagcgtctcg cgcagtacgt cgcgcgcggg cggtgcgagc gccacctgcg gttcgcgctc
     1681 ttcaagtcgg aggcgcaaag cctgatgcgg cgctacggcc ttgaggccga gccgctgtcg
     1741 ccgctgctct cgggcgcggg ccagcgcttc gaggaggagc gcgtcgcgga actggccgaa
     1801 ggcgccgagg tgcgcgacct gcgcggccag tcgtgcgaaa actttatcgc cgcggtgaag
     1861 tcgcagggcg cggggcgcgt cttctactac cagccgacgc tcgtgggccg catcggcggc
     1921 tgggcgtgcg aggggcgcgc ggacttgatc gaggtcacgc gcgagggcgc gcgcgtcgag
     1981 gcgacagtcg tcgatttcaa ggcgagcgcg cgcgagtcgg tcggcttccg tttgcaggtc
     2041 gcgttctacg cgcggctgct cgcgggcgcg ctcgccgcgg cgggcttcga gggcttcacc
     2101 gtgcgcggcg ccgtcgccgc gcgcgacagc cgccagacgc ccgaaggcgg ctgggagacc
     2161 ttcgacctcg cgctctacct cgatgagatc gagagcctcg tggccgcgcc cgactcggac
     2221 gtggcacgcg cggcgcgcgc cgtcggcggc acggcgcgtt atcacctgcg cgcctcgtgc
     2281 gacggctgcc cctacaacgc gctctgcttc gcggacaccg ccgagcgcga agacctctcg
     2341 ctcgtccctc tgctcacgcc gtcggagaag cgcgcgctcg aagccgaggg cgtcaccacg
     2401 gcccgcgcgc tcgccgcgct gatgcgctac ggcgcgggcg cgatggagcc ggcgcccggc
     2461 gccgaagagg aggcgcgcag ggtctccgcc cgctggccgc tcacggggcg gctccccacc
     2521 ctcgtccagc gcgcgcgcgc cgccgtcaac cgcttcgaca agaccgtcaa cacgcgccgc
     2581 tacctcgtcg gcgcggacta cggctcgctg cccgacgagg cgcaacaccc cggcctcgtg
     2641 cgcgtcttcg tggacgcgca gcgcgactac ctcaaggaca aactcttcct cgtcgcggcg
     2701 cgcgtcgccg ggccgcgcgg cacgttcgag ctggtcgaga tgacggacgc cccgcccgac
     2761 gccgacacgg agcgcgcgat gctcctcgcg tgggtgcgac gccttctgcc cgccatcgcc
     2821 gacgcggcag actcgcagcg cgcgccgctc cacttctaca ccttcgcgcc gcgcggcgag
     2881 cgcgcgctcc tcgacgcgct cgcgcgccac ttcgacgcgc tctgcgccgt ccccgccttc
     2941 tacgacctct tgacttcgca ccccgcgctc tcgcagccga tggtctcgct cctctccgaa
     3001 gaggtgcgcg cgcgcctgaa cctcgcgccc gtctgccaca acctctacga agtcgcccgc
     3061 gccctcggct tcgagtggcg cgaccgcgac acggacttcc gccagacctt ccgcgcccgc
     3121 gtcttcgaca accgccgcgc cttcgagcgc gaccgcgcca cgggacgatt caagaccgag
     3181 gggaaagggg gaaaagggga agaggcgaaa aggttgaaag gggaagaggg gaaaagggaa
     3241 gacgacaagc acgctgcctc tcatcaagtc gatgacgaca ggcacgctgc ttctcatcgt
     3301 caacttgatg agaggcaccg tgcttctcat caagttgacg agaagcacgg tgcttctcat
     3361 catccctctt cgcctttcga cctcttcccc ttttctccct ccgacgacga agacgcgccg
     3421 ccgcccttcg tctacgtcga gtcggcggcg cgcttcggcg cggagattcc gctcgaatac
     3481 gcttacgccg cgtggggcgc tctgcgggaa ccgaacgagg cgaccgggcg cgagcgttcg
     3541 caagtaaaag gctttctcgg cgtgagcggc gagcagcttc gcgccttcgc cgcgcaccgc
     3601 acgcgcgcca tgcagcacgt cgaagagagc ttcgcgtaca agaaccgccg cgtcgagaaa
     3661 gagccgctcg acctctcgcg cctgcacgaa gtttcggtcg cgcccgaaga ggtgccgctc
     3721 caccgctcgc tcgaagactt cctcctgctc gaacaccacg cctcttttca ggagcggctc
     3781 ctgcacctct cgcagccgcc gcgcgagcgc gccgcgacgg gccgtgccgt cgtcctgcgt
     3841 tgcgagagct atgagaagga cgaagagcga gtcgagcgcg cttccctctg cttcgccgac
     3901 gccgaaggcc gcgagcagac cgtcaaagac ctcggcctgc tgcgcctgcg cgcgggcgac
     3961 tggacggcct tgaacccgct cgccgacgcc gaagggcgcg cgctccccgc gtggcgcctg
     4021 gtgcgcgggc gcctcgccgt cgtcgaagag ttgacggacg cgggactctc tctgcgcctc
     4081 atgccgatga gcttcaagaa ctcgcccttc cgctacccgc acggcctctt cgcgccgacg
     4141 tcgggcgcgc tctacacgct cgacgagatg gccgacgacc tcaacgccga caagttcctc
     4201 gaagcctgcc gccacgcgcc cacgaaccac ctctaccgct ggctcgaaga cgcggaagaa
     4261 ggcaggcgtc cccgcgcgct gcggcccaag cggctccgcg acgcgctcga agtcgctgcg
     4321 ctcgccgcac gcacgcaaca gccgcacggc ctcaccgcag cgcagaccga gatcgtcggc
     4381 tcacatctcg aagaccgcgt cctcgtcgtg caaggcccgc ccggcacagg caagagccac
     4441 acgctcggct tcgccgtcct cgcccgcgcc ctcgcgcaga gcacccccgc gcgccccttc
     4501 cgcgtcgccg tcctcgcgcg cacgcacgcc gccgtcaatg tcgccctctc ttcgatcgcc
     4561 aaacgcaccc gcgagctttt agaagttgat gaagagcacg gtgcctctca tcaatcgtca
     4621 gcgctacagc tcgcgccgct cgcgcggctg cgggtcgtga aggtgtgcaa tgacgcgggc
     4681 gaggcggtgc ccgaaggagt cgggcgcgtg ttgcccggcg gcgacgaaga ggcgagcgcc
     4741 gcggagcagt gggagcaact tctcggcgaa gaactgctcg tcgtcggcgg cacgcccggc
     4801 ggtctctatc gtctggtgcg cgcgggcgcg gcccgcgggc gcaaggtgaa ctgggcggaa
     4861 aaatacttcg acctcgtcgt cgtggacgag gcttcgcaga tgggactcgc ggaggcgctg
     4921 accgccgccg ccttcctgcg cgacgacggg cagttcatcg ccgtcggcga ccaccggcag
     4981 atgccgccca tcctcgcgca cgcgtgggac agggaagcgc ggcgcgacat ccggcgcgcg
     5041 cgcccgcacc tctccgtctt cgaatacctt ctggaggcgg gcttcgctcg cgccgcgctc
     5101 gacgagtcgt tccgcatccc ggcggaggtc gccgactttc tctcgcgcca cgtctacgcc
     5161 gcggacggca tcgacttccg ctcctcgaac cgcaagcggc tcgccgccgc cgaaggcatc
     5221 cgcgaagact ggctgcgcgc cgcgctcgcg cccgaacacg cgctggtcgt cgtcgagcac
     5281 gacgaggccg gctcgcagca gtcgaacgag ttcgaggcat cgctcgtcga agcaatcgcg
     5341 cgcgccgctg cggagcagtt aagcctcgac gcgcgtaaag gtctcggcgt cgtcgtcccg
     5401 caccgcgcgc agaaggctct gctcgccgcg cgcctgcccg cgctcgccga ctccatcgac
     5461 acggtcgagc gctttcaggg cggcgagcgc gacctcatcg tcgtctcggc cacggtctcc
     5521 gaccgcgagt tcgccgcggg cgagagcggc ttcctgctcg aaccgcgccg cctcaccgtc
     5581 gccgtctcgc gcccgcggcg caagctcatc gtcgtcgcct cgcgcgccgt cttcgacctc
     5641 atccccccgg acctcgacga ctacgaacgc ggcgccctct ggaaacacct ccgccgcgaa
     5701 gccagcgcgc gcctgctctg gcagggcgaa gtcgccggcc accgcgtcac cgtgcgcgcc
     5761 atttagggcg ggtttcgtct gaacctgccg agagatggaa gaccgtaggg gcagggcttg
     5821 tccctgcccg cgatgagtta acacccaggt gagtgtggag cacgcggcgg gtgattgtca
     5881 ttttcgggcg ggggatgtta tatctccact tcatccagct tggaatagtt ttgctgccca
     5941 acgtccggcg ggtagaggcg cgcggcggtc cttctttgaa ttcaactgaa actcggaaag
     6001 gtgtgggagt catcatggct gagaagacgc ggattacggt ggcgcatggg gacgggatcg
     6061 ggccggagat tatgggggcg acgctgagga ttttggaggg ggcgggcgcg cggctcgaaa
     6121 tcgaggagat cgagatcggg gagaaggttt atctggcggg gaacccggcg gggatcgcgc
     6181 cggaggcgtt cgagagtttg cggcggacga aggtgtttct gaaggcgccg atcacgacgc
     6241 cgcagggcgg tgggtataag agcctgaacg tgacgatcag gaagacgttc gggctgtacg
     6301 cgaacgtgag gccgtgcgtg tcttatcaca cgttcatcga gacgaagcat ccggtgatgg
     6361 acgtggtgat cgtgcgcgag aacgaggagg acgtgtacgg gggcatcgag taccggcaga
     6421 cgaagcaggt gacgcagtgt ctgaagctga tctcgcggcc cggcacggag aagatcgtgc
     6481 ggtacgcgtt cgagtacgcg cgggcgaaca accgtcggaa ggtgacgtgc ttcacgaagg
     6541 acaacatcat gaagatcacg gacggcctct tccacaaggt gttcgacgag gtcgccgccg
     6601 ggtacacgga catcgagaac gagcactgga tcgtggacat cggctcggcg aagctggcgg
     6661 acacgcccga ggctttcgac gtggtggtgc tgccgaacct gtacggcgac gtgctgtcgg
     6721 acgtggcggc gcagatcgcg gggtcggtcg gactcgcggg gtcggccaac atcggcgagt
     6781 cgattgcgat gttcgaggcc atccacgggt cggctccgcg gcgcgcgggg cagaacgtgg
     6841 cgaacccttc ggggctgctg ctcggcgcgg tgatgatgct cgtccacatc gggcaggggg
     6901 acatcgccga gcgcgtgcac aacgcctggc tgcgcacggt cgaggagggc atccacacgt
     6961 acgacattta taaagagggc gtcagccgcg agaaggtcgg gacgcgcgag ttcgccgaag
     7021 cggtcgtcgc gcgcatgggg cagaggccgg agacgctgaa ggccgtgagc tacaaggcgg
     7081 gcgcgccggc ttcattgacg ggcggcaaga cgagcgccgc ctcttcggag acgaaggcgc
     7141 tggcgggcgt ggacgtgtac ctcgacttct cgtccggcac gtcggacgag ctgggcgcgg
     7201 cgatggagaa tctggcgggc gacggcctgc gcatctcggc catctcgaac cgcggcgtga
     7261 aggtctggcc gggcggcaac cccgagacgt tctgctccga ccactggcgc tgccgcttcg
     7321 tcgccgacgc ggacggcgcc gccgtgacgc acgcgcaggt cgccgcgctc ctcaagcgcg
     7381 cggcggaagc gggccacgac ttcatcaaga ccgagaacct ctacaacttc gacggcaagc
     7441 ccggctactc gcacgcgcag gagtaggaaa gaaatgatga atgcggaatg atgaatgatg
     7501 aataagaaac agcttgagtg atgaaaagca acgtgccttt cattcatcat tcatcattcc
     7561 gcattcatca ttcatcattc aaaggagaga cagagatgtc tgcaacggcc cccgcgcgt
//
LOCUS       DBNGOH010000783         3785 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4883259, whole genome shotgun sequence.
ACCESSION   DBNGOH010000783 DBNGOH010000000
VERSION     DBNGOH010000783.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3785)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3785)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3785
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4883259"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..736)
                     /gene="era"
                     /locus_tag="AC159P_15070"
     CDS             complement(<1..736)
                     /gene="era"
                     /locus_tag="AC159P_15070"
                     /inference="COORDINATES: protein motif:HMM:NF000908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HIR3696821.1"
                     /translation="MTEETEQGERQESDYRSGYVALVGRPNAGKSTLLNRLVGEKIAA
                     VSNKPQTTRHRIQGVITRPEGQIVLVDTPGVHQPGYLLNRRMMAAVHDALDGVDLLLL
                     IRDATAPTGNGDRFVLDLVKQSAKPALLLLNKTDKLEDKSKLLALIEWYTKEHEWRGV
                     VPLSARKGDQVDVLMREVIKNLPEGEPLFEGDELTDQPVRVLVAELVREKILRVTGEE
                     IPYVTAVVTERYEEISAEMARIYCAIF"
     gene            complement(753..2318)
                     /locus_tag="AC159P_15075"
     CDS             complement(753..2318)
                     /locus_tag="AC159P_15075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019947723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HIR3696822.1"
                     /translation="MRRFISLSIVLVFALAPVARAQERVDHQVIARIKTEAFQNSKVM
                     ETLFYMTDVHGPRLTGSPQLKAASEWARGRLAGWGLADARLEPWGTFGRGWSIKRYSV
                     EMTAPQYMNVTAMPKAFTPGTNGPVSGTPVIVEIRTKPDLDKYRGKLRGKIVMNGRPT
                     NNNVRFEPYAKRMTDDELKKSAETMNPGEPKSFLDEDKQWQESLKWQDEVNKFYHDEQ
                     IAVLLQPSPQSSGVINITAQSYTPNDPMQTFPAVVLAREHYGRIMRLVEKNIPVTLSV
                     NVETQFHTEDQTGYNVLADLPGTDPRLKDELVMLGGHYDSWHAATGATDNAAGCAVML
                     EAMRILKTLGVRPRRTIRVALWTGEEQDYYGSISYVKKHFGDPQTLKLLPGHEKLSAY
                     FNLDNGAGKIRGVNLQGNEAVRPIFDAYLQPFHYLGATTLTTANTGGTDHMAFEALGL
                     PGFQFIQDPLEYNTRTHHTNLDFYEAVVEDDLKQAAAVIASFVYHTAMRDERLPREAL
                     PKLPPPDTKASGQ"
     gene            complement(2412..3587)
                     /locus_tag="AC159P_15080"
     CDS             complement(2412..3587)
                     /locus_tag="AC159P_15080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004616623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HIR3696823.1"
                     /translation="MINQERIKNLLLELVRIDSISRKERDVAERVKQLCEEMGAEVET
                     DDAGRKVGGNTGNVIARFPGTIPHAEAVMMSAHMDTVVPGEGVKPVVEGDIIRTDGTT
                     VLGGDDKSGIAVIIETVRCLQEQAIPHAPIDAVFSICEEVGLLGAKHLDLAKVRARYG
                     IVFDSDDPGFLFTKGPSANHFEFRVHGLESHAGVAPEYGISAIKIAAEGIAAMKLGRI
                     DDETTANIGVISGGEATNIITNLVTLRGEARSRSEEKLEAQTEHIRRCLEEAAARYEV
                     TVEGVTTRARVESHVNREYYAMDVGEDSRVVRLVKEAGARLGLAVETMASGGGCDANI
                     FNRRGIECANLGTGMRAIHTVKEWLDVKDMYASAEMALEIMRLNGEQAMKTDGGKGR"
BASE COUNT          542 a         1234 c         1252 g          757 t
ORIGIN      
        1 cgaagatggc gcagtagatg cgcgccatct ccgcgctgat ctcctcgtac ctttcggtca
       61 cgaccgccgt cacgtacgga atctcctcgc ccgtcacgcg caggattttc tcgcgcacca
      121 gctcggcgac cagcacgcgc accggctggt cggtcaactc gtccccctcg aacagcggct
      181 cgccctccgg caggttcttg atgacctcgc gcatcagcac gtccacctga tcgcccttgc
      241 gcgccgagag cgggacgacg ccccgccact cgtgctcctt cgtgtaccac tcgatgagcg
      301 ccagcagctt cgacttgtct tccagcttgt ccgtcttgtt cagcagcagc agcgccggct
      361 tcgccgactg cttcacgagg tcgagcacga agcggtcgcc gttccccgtc ggcgccgtcg
      421 catcgcggat cagcagcagc aaatcgacgc cgtccagcgc gtcgtgcacc gccgccatca
      481 tccgccggtt caaaaggtat cccggctggt gcacccccgg cgtgtcaacc aacacgatct
      541 gcccctcggg ccgcgtaatc acgccctgaa tccggtgccg cgtcgtctgc ggcttgttcg
      601 acaccgccgc gatcttctcg cccaccagcc gattcagcaa agtggacttc cccgcgttcg
      661 gtcgccccac cagcgcgacg taaccgctcc ggtaatcgct ttcctgacgt tcgccttgtt
      721 cagtctcttc cgtcataacg caacttcgtt tctcattgcc cgctggcctt cgtgtcgggc
      781 gggggcagtt tggggagggc ttcgcggggg aggcgttcgt cgcgcatggc ggtgtggtag
      841 acgaaggagg cgatgacggc ggcggcctgt ttgaggtcgt cttcgacgac cgcctcgtag
      901 aagtcgaggt tggtgtggtg cgtgcgcgtg ttgtattcga gggggtcctg gatgaactgg
      961 aagccgggca gaccgagggc ttcgaaggcc atgtggtcgg tgccgccggt gttggcggtc
     1021 gtgagggtcg tcgcgccgag gtagtggaag ggctggaggt aggcgtcgaa gatggggcgc
     1081 acggcctcgt tgccttggag gttgacgccg cgaatcttcc cggcgccgtt gtcgaggttg
     1141 aagtaggccg agagcttttc gtggccgggc aggagcttga gcgtctgcgg gtcgccgaag
     1201 tgctttttga cgtagctgat ggagccgtag tagtcctgct cttcgcccgt ccagagggcg
     1261 acgcggatgg tgcggcgggg ccgcacgccg agcgtcttca aaatccgcat cgcttccaac
     1321 atgacggcgc agccggcggc gttgtcggtg gcgccggtgg cggcgtgcca cgagtcgtag
     1381 tggccgccga gcatgaccag ctcgtccttg aggcgcgggt cggtgccggg gaggtcggcg
     1441 aggacgttgt agccggtctg gtcttcggtg tggaactgtg tctcgacgtt gaccgaaaga
     1501 gtgaccggga tattcttctc gacgagacgc atgatgcgcc cgtagtgctc gcgcgcgagg
     1561 acgaccgcgg ggaacgtctg catggggtcg ttcggcgtgt aggactgcgc cgtgatgttg
     1621 atgactccgc tcgattgcgg gctgggctgg agcaggacgg cgatctgctc gtcgtggtag
     1681 aacttgttga cctcgtcctg ccacttcaga gactcctgcc actgcttgtc ttcgtcgagg
     1741 aacgacttcg gctcgccggg gttcatcgtt tcggcggact tcttcagctc gtcgtccgtc
     1801 atccgcttgg cgtaaggctc gaagcggacg ttgttgttgg tcggcctgcc gttcatgacg
     1861 atcttgccgc gcagcttgcc gcggtacttg tcgaggtcgg gcttcgtcct gatctcgacg
     1921 atgacgggcg tgcccgagac ggggccgttc gtgcccggcg tgaaggcctt cggcatggcg
     1981 gtgacgttca tgtactgcgg cgccgtcatc tcgaccgagt agcgcttgat cgaccacccg
     2041 cgcccgaacg tcccccacgg ttcgagccgc gcgtccgcca gaccccaccc ggcgagccgc
     2101 ccgcgcgccc attcgctcgc cgccttcagt tgcggcgagc cggtcaggcg cggcccgtgc
     2161 acgtccgtca tgtagaagag cgtctccatc accttcgagt tctggaacgc ctccgtcttg
     2221 atccgcgcga tgacttgatg gtcaacgcgc tcctgcgccc gcgcgaccgg cgccaaagcg
     2281 aaaacgagga cgatcgaaag agagataaaa cggcgcactt gggaagcctc cttaaaatga
     2341 gacgggggaa ggggagacag ggagaagggg agacgaagac aacatccatc cgtttttgtc
     2401 tccccttctc cctatctccc cttccccccg tctgtcttca tcgcctgctc gccgttgagg
     2461 cgcatgattt cgagcgccat ctcggcggag gcgtacatgt ccttcacgtc gagccattcc
     2521 ttgacggtgt ggatggcgcg catgccggtg ccgaggttgg cgcactcgat gccgcggcgg
     2581 ttgaagatgt tggcgtcgca gccgccgccc gaggccatcg tctcgaccgc gaggccgagc
     2641 cgcgcgcccg cctccttgac gaggcgcacg acgcgcgagt cttcgcccac gtccatcgcg
     2701 taatactcgc ggttgacgtg cgactcgacg cgcgcgcgcg tggtcacgcc ctcgaccgtg
     2761 acttcgtagc gggccgcggc ttcttcgagg cagcgccgga tgtgctcggt ctgcgcttcg
     2821 agtttctcct cgctgcgcga gcgcgcttcg cccctgaggg tgacgaggtt ggtgatgatg
     2881 ttcgtggcct cgccgccgct gatgacgccg atgttggcgg tcgtctcgtc gtcgatgcgc
     2941 ccgagcttca tcgcggcgat cccttccgcc gcgatcttga tggccgagat gccgtactcg
     3001 ggcgcgaccc cggcgtgcga ctcgaggccg tggacgcgga actcgaagtg gttcgccgaa
     3061 ggccccttcg tgaacaggaa gccggggtcg tccgagtcga agacgatgcc gtaacgggcg
     3121 cgcaccttcg cgaggtcgag gtgcttggcg ccgagcaggc cgacctcctc gcagatggag
     3181 aagacggcgt cgatcggcgc gtgcgggatt gcttgttctt gcaggcagcg gacggtctcg
     3241 atgatgacgg cgatgcccga cttgtcgtcg ccgccgagga cggtcgtgcc gtcggtgcgg
     3301 atgatgtctc cctcgacgac gggcttcacg ccctcgccgg gcaccaccgt gtccatgtgg
     3361 gcgctcatca tgacggcctc ggcatgggga atcgtgcccg gaaagcgcgc gatgacgttg
     3421 cccgtgttgc cgcccacctt gcgccccgcg tcgtccgtct cgacctccgc gcccatctct
     3481 tcgcagagct gtttgacgcg ctcggccacg tccctttctt tccgcgagat gctgtcgatc
     3541 cggacgagtt cgagcagcag gttcttgatg cgttcctgat tgatcatctg gcctccgtga
     3601 cccgtggggc gggcggcgcg gcgaatcccc tgtccccgcc tcgcgtcgcc ccacgcccgc
     3661 cacctgtcat gaattttcgc catcctacta caggccgtcg ggccgttgcc acacaaagcg
     3721 ccgccgcgtt cggcgctttg tgtggcaaga cagagaagtc cgacacccga ccgctcggcg
     3781 cggtc
//
LOCUS       DBNGOH010000784         3796 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_667483, whole genome shotgun sequence.
ACCESSION   DBNGOH010000784 DBNGOH010000000
VERSION     DBNGOH010000784.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3796)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3796)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3796
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_667483"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(77..1234)
                     /gene="ftsZ"
                     /locus_tag="AC159P_15085"
     CDS             complement(77..1234)
                     /gene="ftsZ"
                     /locus_tag="AC159P_15085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005811376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HIR3696824.1"
                     /translation="MTPEGRLPGSAINIIIDDEPPITGARIKVIGVGGGGGNAVNRMI
                     EAGIEGIEFLVANTDLQALKRSRAPIKIQLGGKLTKGLGAGANPDIGRNAALEDTEKI
                     IEALEGADMVFVTTGLGGGTGTGAAPIIASLATELNALTVAVVTKPFHFEGKRRMAQG
                     DQGLRELRECVDTVITIPNERLLHTVDRNVSLQDAFKLADDVLRQAVQGISDLIIVPG
                     LINLDFADVKTIMAGMGLALMGAGRATGENRALEATQQAISSPLLEEATIEGAKGVLI
                     NITGGMDLTLYEVNEASSIIREAADDDANIIFGAVIDETLRDEMKITVIATGFDKEIA
                     ELGTTPHTSAAAPAPSSRYAATSAADVAPRGAAPARPDDLDVPTFIRKKAD"
     gene            complement(1376..2719)
                     /gene="ftsA"
                     /locus_tag="AC159P_15090"
     CDS             complement(1376..2719)
                     /gene="ftsA"
                     /locus_tag="AC159P_15090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005987962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HIR3696825.1"
                     /translation="MTKLVSHTVGLDIGTSRVTCIIGEPAEGGTVNIVGIGEAESRGL
                     RKGVVVDPEAAVDSVTRAVEEAERMSGLEAEEVAINLSGSHIMSFNGQAIVAVSGRER
                     EPSHQNGHGNGTRRGHHTMTVVAGRDREITHDDVRRAIESASAIQLPAGREIVDRLPQ
                     EFIVDDQDGINDPVGMIGARLAVKLHIVTSPVTARQNVINGVNRAGLVVGEMVLEQLA
                     AAEATLTDDDKEFGAALVDIGAETTGLVIYQRGAVQHTAVFALGGSHFTNDIAFGLRT
                     PIPEAEKIKRTVGCASSMSLTEMERGELIEVPSVGGRPPRQLSRQILCDILQPRAEEV
                     LSHVADEIRDSGWAGQISSGVVLTGGGSLLDGMVEIAEQVFDAPVRLGYPERDRFGGL
                     VEDVQSPAWAAAAGLCLMAQRAKSAEMRASLARSNGAGGFGALVNKFRNRFGGIF"
     gene            complement(2756..3742)
                     /locus_tag="AC159P_15095"
     CDS             complement(2756..3742)
                     /locus_tag="AC159P_15095"
                     /inference="COORDINATES: protein motif:HMM:NF015739.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HIR3696826.1"
                     /translation="MEVISVGLLFLLLLGLLAVVVGVVAFVGYRTAASASFFKVRTVD
                     VEGNARSSRKDIEEVVTGIAGRTGVWRADLETISEEIERLPWVRSAVVTRVLPSGVRV
                     RVTERAPAVIARTQAGRLVWVDDEGVVLGAASPGEDEFFMRGLDEGRGDDARQKNRER
                     VRVATGLAREWEAASLSRRVSEINLEDLRDVRVQLAGADAHVEVRLGKEDFARRFRQA
                     LEVLDAQRGTARGPFVTYVDVSQGKRAVVGTGTSAHVPIESSEQQAPAAEPPSAEGAR
                     AAESPARPEATRAAAKRADERKGVKKKTETGRDRRGAAERRAEDATRPRRVG"
BASE COUNT          533 a         1357 c         1248 g          658 t
ORIGIN      
        1 gggtaagaac cacctcttac ccggcgcccg gcgcctgaca cccgaaaccc tctgctctgc
       61 tgtcagtcgc ctttcctcaa tcagcttttt tgcggatgaa ggtcggcacg tcgaggtcgt
      121 cggggcgggc cggcgcggcg ccgcgcgggg cgacgtccgc agccgaggtc gcggcgtagc
      181 gcgaggaggg cgcgggcgcg gcggccgagg tgtgcggcgt cgtcccgagt tcggcgatct
      241 ccttgtcgaa gccggtggcg atgacggtga tcttcatctc gtcgcgcagc gtctcgtcaa
      301 tgaccgcgcc gaagatgatg ttggcgtcgt cgtcggccgc ctcgcggatg atcgagctgg
      361 cctcgttgac ctcgtagagc gtgaggtcca tcccgcccgt gatgttgatg agcacgccct
      421 tcgcgccctc gatggtcgcc tcttcgagga gcgggctgga gatggcctgc tgcgtggctt
      481 ccagcgcgcg gttctcgccc gtcgcgcgcc ccgcgcccat cagggcgagc cccatcccgg
      541 ccatgatggt cttcacgtcg gcgaagtcga ggttgatgag gccgggcacg atgatcaagt
      601 ccgagatgcc ctgcacggcc tggcgcagca cgtcgtcggc gagcttgaac gcgtcctgca
      661 aagagacgtt gcggtcaacc gtgtggagca gccgctcgtt cgggatcgtg atgaccgtgt
      721 ccacgcactc gcgcagctcg cgcaagccct gatcgccctg cgccatgcgg cgcttgcctt
      781 cgaagtggaa cggcttggtg acgacggcga ccgtcagcgc gttcagctcc gtcgccaacg
      841 aggcgatgat cggggcggcg ccggtgcccg tgccgccgcc gaggcccgtc gtgacgaaga
      901 ccatgtcggc gccttccaaa gcctcgatga tcttctccgt gtcttcgagc gcggcattgc
      961 gaccgatgtc ggggttggcg cccgcgccca agcctttggt gagtttgccg ccgagctgaa
     1021 tcttgatcgg cgcacgcgaa cgtttcagag cttgcaggtc ggtgttggcg acgaggaact
     1081 cgatgccctc gataccggcc tcgatcatgc ggttgacggc gttgcccccg ccgcctccga
     1141 cgccgatgac cttgatgcgc gcgcccgtga tgggcggctc gtcgtcaatg ataatgttga
     1201 tcgcgctgcc cggcaggcgg ccttccggtg tcataattgc agtcctctct ccttcggaag
     1261 cttcatgcgg cctcgaaagg gctgtggcgg ttatcttggg ctgtgcgcgc cggcctcaac
     1321 aatatattga gggctagata ttgtgccggc tggcgcggca gcatacaaca tcttctcaga
     1381 aaataccacc gaaacgattg cgaaatttgt tgacgagcgc gccgaaaccg cccgccccgt
     1441 tggagcgcgc cagcgacgcg cgcatttccg cggatttggc gcgctgcgcc atcagacaga
     1501 ggccggcggc ggcggcccac gctggactct gtacgtcttc gaccagcccg ccgaagcggt
     1561 cgcgctccgg atagccgagg cgcacgggcg cgtcgaagac ctgctccgcg atctcgacca
     1621 tgccgtccaa gagagagccg ccgcccgtca gcacgacgcc gctcgaaatc tgaccggccc
     1681 aacccgaatc cctgatctcg tcggcgacgt gcgacaagac ttcttccgcg cgcggctgaa
     1741 ggatgtcgca caaaatttgc cgcgagagtt gtcgcggcgg gcgcccgccc accgaaggca
     1801 cttcgatcaa ctcgccgcgc tccatctccg tcaagctcat cgacgacgcg cagcctaccg
     1861 tgcgcttgat cttttccgcc tccggtatcg gcgtgcgcag gccgaaggcg atgtcgttcg
     1921 tgaagtgcga gccgccgagc gcgaagaccg ccgtgtgctg cacggcgccg cgctggtaga
     1981 tgacgaggcc cgtcgtctct gcgccgatgt ccacgagcgc cgcgccgaac tccttgtcgt
     2041 cgtcggtcaa cgtcgcttcc gccgccgcga gttgttcgag aaccatctcg ccgacgacga
     2101 ggccggcgcg gttcacgccg ttgatgacgt tctgccgcgc ggtgacgggg ctggtcacga
     2161 tgtgcagctt gacggcgagc cgcgcgccga tcatgcccac ggggtcgttg atgccgtcct
     2221 ggtcatcgac gatgaactcc tgcggcaggc ggtcaacgat ctcgcggccc gcgggcagtt
     2281 ggatggcgct cgcggactct atggcgcggc gcacgtcgtc gtgcgtgatc tcgcggtcgc
     2341 gcccggcgac gacggtcatc gtgtggtggc cgcgccgcgt gccgttgccg tggccgttct
     2401 ggtgcgaagg ctcgcgctcg cggcccgaga cggcgacgat ggcctgcccg ttgaagctca
     2461 tgatgtgcga gccggagagg ttgatggcga cctcttcggc ttcgagtccg ctcatccgct
     2521 cggcctcttc gacggcgcgc gtgaccgaat cgaccgccgc ctcgggatcg acgacgacgc
     2581 ccttgcgcag gccgcgcgac tccgcctcgc cgatgccgac gatgttgacc gtcccgccct
     2641 ctgccggctc gccgatgatg caggtcacgc ggctcgtccc gatgtcgagt ccgacggtgt
     2701 gcgacactag tttcgtcatt cggtttcagt ccctccgcaa acgagtgctc cgccttcagc
     2761 cgacgcggcg cgggcgggtc gcgtcttccg cgcggcgctc ggcggcgccc ctcctgtcac
     2821 gccccgtctc cgtcttcttc ttcaccccct tgcgttcgtc cgccctcttc gccgccgccc
     2881 tcgtcgcctc gggtcgcgcc ggactctccg ccgcgcgcgc gccttccgcc gacgggggtt
     2941 cggcggcggg cgcctgctgt tcggaactct cgattggcac gtgcgcgctc gtgcccgtgc
     3001 cgacgacggc gcgcttgcct tgcgacacgt caacgtaggt gacgaacggg ccacgcgccg
     3061 tgccgcgctg ggcgtcgagg acttcgagcg cctgccggaa gcgccgcgcg aagtcctcct
     3121 tgccgagccg cacctcgacg tgcgcgtcgg cgcccgcgag ctgcacgcgc acgtcgcgca
     3181 ggtcttcgag gttgatctcg ctgacgcggc ggctcaggct cgccgcctcc cactcacgcg
     3241 ccagcccggt cgctacgcgg acgcgctcgc ggttcttctg ccgcgcgtcg tcgccgcgcc
     3301 cctcgtccag tccgcgcatg aagaactcgt cctcgccggg cgaggccgcg ccgagcacga
     3361 cgccctcgtc gtcaacccac acgaggcggc ccgcctgcgt gcgcgcgatg acggcgggcg
     3421 cacgctccgt cacgcgcacg cgcacgcccg aaggcaggac gcgcgtgacg accgccgagc
     3481 gcacccacgg cagccgctcg atctcttcgc tgatcgtctc aaggtctgcg cgccacacgc
     3541 ccgtccggcc cgcgatgccc gtcacgacct cctcgatgtc cttgcgcgaa gagcgcgcgt
     3601 tgccttccac gtcaaccgtc ctcaccttga agaacgacgc cgaagcggcg gtgcgataac
     3661 cgacgaaggc gacgacgccg accacgaccg ccagcaggcc gagcagcaga aggaacagca
     3721 acccgacgga gatcacttcc atctcccctc tcccgcgtta cccgccggcg ccgaccaccg
     3781 cgccgggggc gaccac
//
LOCUS       DBNGOH010000785         1890 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3968800, whole genome shotgun sequence.
ACCESSION   DBNGOH010000785 DBNGOH010000000
VERSION     DBNGOH010000785.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1890)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1890)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1890
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3968800"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1706)
                     /locus_tag="AC159P_15100"
     CDS             complement(<1..1706)
                     /locus_tag="AC159P_15100"
                     /inference="COORDINATES: protein motif:HMM:NF042645.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6600 domain-containing protein"
                     /protein_id="HIR3696827.1"
                     /translation="MRPRTRLHAALLFLLSTIILFPSHTFAAEDEYEDEYEEQARVVR
                     VSMMKGEVSLRRAGNIEWERAQLNLPLVEGDVLATGRDSHVEVQIDAKNFVRVGDESV
                     LKVVTLRDEGVALSLSEGTASLRLARFDRDKEYFEIDAPGTTVAAEKTGLYRLDVASD
                     GSVRVTVRDDGRARLYSRDSGFVLRNNKTAKFVFDQKGEADWEFAEAAALDEWDFWND
                     ERERHLAALLRFEERDRYYDREVWGAEELDSYGDWMHTREYGYLWRPHVTVINNYYNW
                     APYRYGHWRWCPPYGWTWIPDEPWGWAPYHHGRWVYYNNNWCWAPRGYGYHYRRAWWR
                     PALVAFIYIPTSHGEHIAWYPLRHGQRDPRGRHWGRNRDGFSPGGRPRESATVRGFDP
                     GILRGVSSVPARDFGVAAVRAKPASNEIAQRALNGEPVPGHLPVTPPDLTRIVSPDGG
                     VRTKGSASRATGAGERPTLDIKRAAPTGPPRALPERTTGAAARTPGVPLDGELRRTRV
                     FGNREPRVAAPNVGTVDRGDEGTGAVTRPAPGARRKPVEGAGDSRDDGGANTFPRTRP
                     ARP"
BASE COUNT          252 a          614 c          707 g          317 t
ORIGIN      
        1 gggcgcgcgg gacgcgtgcg cgggaaggtg ttggcgcccc cgtcgtcgcg gctgtctccg
       61 gcgccttcga cgggcttgcg gcgggcgccc ggcgcgggcc tcgtcacggc gcccgtgcct
      121 tcgtcgcccc tgtccacggt gccgacgttg ggcgcggcga cgcgcggctc gcggttgccg
      181 aagacgcgcg tgcgtcgcag ctcgccgtcg agcgggacgc cgggcgtgcg cgcggcggcg
      241 ccggtcgtgc gttcgggcag cgcgcgcggc gggccggtgg gagcggcccg tttgatgtcg
      301 agcgtcggtc gctcgcccgc gccggtcgcg cgcgacgcgg aacccttcgt gcgaacgccg
      361 ccgtccggtg aaacgatgcg cgtcaggtcg ggcggcgtca cgggcagatg accgggcaca
      421 ggctcgccgt tcaaggcgcg ctgcgcgatc tcgttcgacg cgggcttcgc gcgcacggcg
      481 gcgacgccga agtcgcgcgc gggcacgctc gacacgccgc gcaggatgcc ggggtcgaag
      541 cctctcacgg tcgcggactc gcgcggtcgc ccgccgggcg aaaagccgtc gcggttgcgt
      601 ccccagtgtc gcccgcgcgg gtcgcgttga ccgtgcctga gcggatacca ggcgatgtgc
      661 tcgccgtgcg aggtcgggat gtagatgaag gcgacgagcg ccggtcgcca ccacgcgcga
      721 cggtagtggt agccgtagcc gcgcggcgcc cagcaccaat tgttgttgta gtaaacccag
      781 cgaccgtggt gataaggcgc ccagccccac ggctcgtccg gtatccacgt ccagccgtag
      841 ggcgggcacc agcgccagtg gccgtagcgg tagggcgccc agttgtaata gttgttgatg
      901 acggtgacgt gcgggcgcca gaggtagccg tactcgcgcg tgtgcatcca gtcgccgtag
      961 ctgtcgagtt cttcggcgcc ccacacctct cggtcgtagt agcggtcgcg ctcctcgaag
     1021 cgcaagagcg cggcgaggtg tcgctcgcgc tcgtcgttcc agaagtccca ctcgtcgagc
     1081 gccgccgcct ctgcgaactc ccagtcggcc tcgcccttct ggtcgaagac gaacttcgcg
     1141 gtcttgttgt tgcgcaggac gaagccggag tcccgcgagt agaggcgcgc gcgcccgtcg
     1201 tcgcggacgg tgacgcggac gctcccgtcg ctcgccacgt ccagacggta gaggccggtc
     1261 ttctcggcgg caacggtcgt gcccggcgcg tcgatctcga aatactcttt gtcgcggtcg
     1321 aagcgcgcga gacgcagact ggcggtgcct tcggagaggc tgagcgcgac gccttcgtcg
     1381 cgcagggtga cgaccttcag cacggactcg tcgccgacgc ggacgaagtt cttcgcgtcg
     1441 atctgcacct cgacgtgcga gtcgcgcccg gtcgccagca cgtcgccttc gacgagcggc
     1501 aggttcagtt gcgcgcgttc ccattcgatg ttgcccgcgc ggcgcaggct gacctcgccc
     1561 ttcatcatgc tgacgcgcac gacgcgggcc tgctcttcgt actcgtcttc gtattcgtct
     1621 tcggcggcga aggtgtgtga agggaagagg atgatcgtgg agaggagaaa cagcagtgcg
     1681 gcgtggaggc gtgttcgtgg acgcataggg cggtgctcct taactgtttt cgagtcggac
     1741 gggagagcgg gccgcttggg ggcatcggga cgcagttcga caaagcgaag ctctacggga
     1801 ctcagcgctt gcgaggggct ttgtacctgc gtcggggcga gtgcaaaggc tgtgcctgtc
     1861 ggaagcggct cggtcgggtg gcttcagata
//
LOCUS       DBNGOH010000786         4229 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_302317, whole genome shotgun sequence.
ACCESSION   DBNGOH010000786 DBNGOH010000000
VERSION     DBNGOH010000786.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4229)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4229)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4229
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_302317"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..763)
                     /locus_tag="AC159P_15105"
     CDS             complement(<1..763)
                     /locus_tag="AC159P_15105"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013617419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004348 - glucosylceramidase activity
                     [Evidence IEA];
                     GO_process: GO:0006665 - sphingolipid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 30 protein"
                     /protein_id="HIR3696828.1"
                     /translation="MSKYLQSLVAPGLILTALLVTAATATAQVVELRGDAGGKRFDGI
                     GVVNGGGATSVLLKDYPEPQRGQILDLVFKPKFGASVSALLVEIPGDGNSTQGSMPSH
                     MHTRHDLDYTRGYTWWVLREAKKRNPNLTLDGTAWSAPGWIGKGGTWAEGEFWSQDAA
                     DYYVKWLRGLRDVYGLEFDAIGCRNEKGYSLAFAKVLRRTLDAGGFGRVKLHAFDHWP
                     DHRFDFVKELLTDKEARDAIDIIGAHVMYERKPASA"
     gene            complement(827..1159)
                     /locus_tag="AC159P_15110"
     CDS             complement(827..1159)
                     /locus_tag="AC159P_15110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696829.1"
                     /translation="MCSWSESISNGSVGRKDSGRLYNFPWVARFHLAMYRATGDSSQL
                     DRFVRVVRSYYKRDGKKHLSMEIPITEGLKTLEEAGRAQEKAELLLRAPLPAGDQRAA
                     RREERPVG"
     gene            complement(1258..>4229)
                     /locus_tag="AC159P_15115"
     CDS             complement(1258..>4229)
                     /locus_tag="AC159P_15115"
                     /inference="COORDINATES: protein motif:HMM:NF051698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HIR3696830.1"
                     /translation="IGLQLAAIRELPGEYFLDNGLIRNLFTQNRVNQNETRYTYKLDH
                     NFTNNFKTSFRYTITPAIGLRGSDIDFEQSGIYSDTKQMLLTFNNILTPTLVNDLRLN
                     YTRANFSEDFAPEFSIVSGRSFSRELGLPALTAGGVPLFLLSGQDGNSYTGANVGNGA
                     STNNFNVEERFNINDIVYWNRGDKTWKMGVDLNLAKLNVIPFFAASGGRWSFRALNNS
                     NNRANNPNGNGGNSYASLLLGVPNAVDIRPLLLDYNYYWKSGAAFVQNDWKLRPNFTL
                     NLGFRYALQYPRAEKNNLQGVFRPDLARTVTLTAAQRQQIATGLAIPITDPFIPTTVS
                     VPPFAFAGRGGRSKYITPVDYFGLEPRFGFAWQPKMKFFGIDTEQHALVIRGGYGISH
                     VPITGNNRSPNPDFGGFTTVGTNLTTGAAPTTGCPTLPIVCASTGTANINQPARLSGN
                     PPLQSNSASLDAILGTDADGLVFDNSIGIPAFADTGFAGSGGKVPYTQTWNLTAQFEA
                     FWGTVVELSYTGNKGTNLYMPLVNINRRNLDFVETLEALSIDATGTFSDPLGRRNLLG
                     AVVAINRASVVTPFFGFDSLNRFFDPSANSIRHAGYIDVRRRVARGVTFTANYTYAKS
                     IDDASDASPDTRVLTTGASRGHVTHGAPRSVDRALSTFDVKNNFASTFIWDLPIGRRR
                     GSTGWLLGNVPGPINTVIGGWTLSGVFRMPGGTPYLPFITDANKLGGVGFNRSVRLDI
                     VEGVPLRNPLWDRDCPTGTNPTTNRPCEPFINPAAFMRPVKGQLGNAPRTVDIRAPRQ
                     EYIDLSLSKDFPWPFANKDGKRRINFRIDAINVFNHPNFRFVGTGNTPPGFGVAPTEL
                     TGETFNGVTQPITIADYNTWANAWNATHPNDTVPLQSTAAGAPINATLQAVRNNVNAV
                     RLPPRAGTVSGALPLDFFHVRIPEGFATSTPESYDIRSLDGFKLYRIRQVYDTSFGTL
                     TPVSNPRYIQFGIRVFF"
BASE COUNT          637 a         1269 c         1554 g          769 t
ORIGIN      
        1 ccgccgaagc gggcttgcgc tcgtacatga cgtgggcgcc gatgatgtct atcgcgtcgc
       61 gcgcctcttt gtcggtgagc agctccttga cgaagtcgaa cctgtggtcg ggccagtgat
      121 cgaaggcgtg aagcttgact cgcccgaagc cgcccgcgtc gagcgtccgg cggagcacct
      181 tcgcgaaggc gaggctgtag cccttctcgt tgcggcagcc gatggcgtcg aactccaggc
      241 cgtacacgtc gcgcaagccc cgcagccact tgacgtagta gtcggcggcg tcctgcgacc
      301 agaactcgcc ctcggcccaa gtcccccctt tcccgatcca gcccggcgcg ctccacgccg
      361 tgccgtcgag ggtgaggttc gggttccgct ttttggcttc gcgcaagacc caccacgtgt
      421 agccgcgcgt gtagtcgagg tcgtggcggg tgtgcatgtg gctgggcatc gagccttgcg
      481 tcgagttgcc gtcgccggga atctcgacga gcagcgcgct gaccgaagcg ccgaacttgg
      541 gcttgaagac gaggtcgaga atctggccgc gctgcggctc ggggtagtcc ttgagcagca
      601 ccgaggtggc gccgccgccg ttgacgacgc cgatgccgtc gaagcgtttg ccgccggcgt
      661 cgcccctcag ttccacgacc tgagcggtcg cggtcgcagc cgtcaccaac agtgccgtga
      721 gaataagacc gggcgcgacg agcgattgaa gatacttgga catggtcgct aaggcttggt
      781 aatagcgcgg acggtgagca gatcgaccaa ggcccagtcc tggtcgttag cccacgggtc
      841 gttcttcgcg gcgggctgcc cgttggtcgc cagcgggtag aggtgcgcgc aggaggagtt
      901 cggccttctc ctgcgcgcgt cccgcttctt ccagggtttt cagcccctcc gtgatcggga
      961 tttccatgct tagatgcttc ttcccgtcgc gcttgtaata actccgcacg acgcgcacga
     1021 agcgatccag ttgcgaggag tcgcccgtcg cccgatacat ggccagatgg aagcgggcca
     1081 cccacgggaa gttgtagagc cgcccggagt cctttcgccc gacggagccg ttcgagatgc
     1141 tttccgacca tgaacacacg ggagacctaa agcggaaggg cggcggattt tcgatccgtc
     1201 cgcccttcca gcgccgcgcc cgagggggcg cggcggctta cggcgcaccg ctcccgttca
     1261 gaagaagacg cggatgccga actggatgta gcgcgggttg ctgaccggcg tcagcgtccc
     1321 gaagctcgtg tcgtagacct gccggatgcg gtagagcttg aacccatcca gcgagcggat
     1381 gtcgtacgac tccggcgtgg acgtggcgaa gccctccggg atgcggacgt ggaagaagtc
     1441 gagcggcagc gccccgctca ccgtccccgc gcgcggcggc agccgcaccg cgttgacgtt
     1501 gttgcgtacc gcctgcaagg tcgcgttgat gggcgcgccc gccgccgtgg actggagcgg
     1561 cacggtgtcg ttcgggtgcg tcgcgttcca ggcgttcgcc caagtgttgt aatccgcgat
     1621 ggtgatcggt tgcgtcacgc cgttgaaggt ctcgcccgtc aactcggtcg gggcgacccc
     1681 gaagcccggc ggcgtgttgc ccgtcccgac gaagcggaag ttcgggtggt tgaagacgtt
     1741 gatcgcgtcg atgcggaagt tgatccggcg cttgccgtcc ttgttggcga acggccacgg
     1801 gaagtccttc gacaaagaga ggtcgatgta ctcctgccgc ggggcgcgga tgtcaacagt
     1861 gcgcggcgcg ttgccgagct gccccttgac cgggcgcatg aaggccgccg ggttgatgaa
     1921 cggctcgcac gggcggttcg tggtcgggtt ggtgcccgtc gggcagtcgc gatcccagag
     1981 cgggttgcgc aacggcacgc cctcgacgat gtcgagcctc accgaacggt tgaagccgac
     2041 gccgccgagc ttgttcgcgt cggtgatgaa cggcaggtag ggcgtcccgc ccggcatgcg
     2101 gaagacgccc gacagcgtcc agccgccgat gaccgtgttg atggggccgg gcacgttgcc
     2161 gagcagccag cccgtgctcc cgcgcctgcg cccgatgggc aggtcccaga tgaaggtcga
     2221 ggcgaagttg ttcttcacgt cgaaggtcga gagcgcgcgg tcaacgctgc gcggcgcgcc
     2281 gtgcgtgacg tggccgcggc tggcgcccgt ggtcagcacg cgcgtgtcgg gcgaggcgtc
     2341 cgaggcgtcg tcgatggact tggcgtaggt gtagttggcc gtgaacgtga ccccgcgcgc
     2401 gacgcgccgt cgcacgtcga tgtagccggc gtggcggatc gagttggccg acgggtcgaa
     2461 gaagcggttg agcgagtcga agccgaagaa cggcgtgacc acgctggcgc ggttgatcgc
     2521 gaccacggcc ccgagcaggt tgcggcgccc gagcgggtcg gaaaaggtcc cggtcgcgtc
     2581 gatgctgagc gcctccaggg tctcgacgaa atcgaggttg cgccggttga tgttgacgag
     2641 cggcatgtag aggttcgtcc ccttgttgcc ggtgtaggag agttcgacga ccgtgcccca
     2701 gaacgcctcg aactgcgccg tcaggttcca ggtctgcgtg tagggcacct tcccgccgct
     2761 gcccgcgaac cccgtgtcgg cgaaggccgg gatgccgatg ctgttgtcga agaccagccc
     2821 gtcggcgtcg gtgccgagga tggcgtcgag cgaagccgag ttgctctgga gcggcgggtt
     2881 gccgctgagc cgcgcgggct ggttgatgtt cgccgtgccg gtcgaggcgc agacgatggg
     2941 cagcgtcggg cagccggtcg tcggcgcggc gccggtcgtc aggttggtgc cgacggtggt
     3001 gaagccgccg aagtccgggt tcgggctgcg gttgttgccg gtgatcggca cgtgcgagat
     3061 gccgtagccg ccgcggataa cgagcgcgtg ctgctcggtg tcgatgccga agaacttcat
     3121 cttcggttgc cacgcgaagc cgaaccgcgg ctcaaggccg aagtagtcca cgggcgtgat
     3181 gtatttcgag cgaccgccgc gccccgcgaa ggcgaacggc gggacgctca ccgtggtcgg
     3241 gatgaacggg tcggtgatcg ggatggcgag gcccgtcgcg atctgctggc gctgggcggc
     3301 ggtcaaggtc accgtccgcg cgaggtcggg gcggaagacg ccttgcaggt tgttcttctc
     3361 ggcgcgcggg tactggagcg cgtagcggaa gccgaggttg agcgtgaagt tgggccggag
     3421 cttccagtcg ttctggacga aggcggcgcc cgacttccag tagtaattgt agtcgagcag
     3481 cagcgggcgg atgtccaccg cgttcgggac gccgaggagc aggctcgcgt acgagttccc
     3541 gccgttgccg ttggggttgt tcgcgcggtt gttgctgtta ttgagggcgc ggaacgacca
     3601 gcgcccgccg gaggcggcga agaacgggat cacgttgagc ttcgcgaggt tgaggtcaac
     3661 gcccatcttc cacgtcttgt cgccgcggtt ccagtagacg atgtcgttga tgttgaaccg
     3721 ctcctcgacg ttgaagttgt tggtcgaggc gccgttgccc acgttggccc ccgtgtagct
     3781 gttcccgtcc tgcccgctga gcaggaagag cgggacgccg cccgcggtca gcgcgggcag
     3841 ccccagctcg cgcgagaagc tgcgcccgct gacgatcgag aactccggcg cgaagtcctc
     3901 gctgaagttg gcgcgggtgt agttgagccg gaggtcgttg acgagggtcg gcgtgaggat
     3961 gttgttgaag gtcaggagca tctgcttggt gtcgctgtag atgccgctct gctcgaaatc
     4021 gatgtcgctg ccgcgcaggc cgatggccgg cgtgatcgtg tagcggaagc tggtcttgaa
     4081 gttgttggtg aagttgtggt cgagcttgta ggtgtagcgc gtctcgttct ggttgacgcg
     4141 gttttgcgtg aagaggttcc ggatcagccc gttgtcgagg aagtactcgc cgggcagctc
     4201 gcggatggcg gcgagttgca gcccgatgg
//
LOCUS       DBNGOH010000787         3894 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_850961, whole genome shotgun sequence.
ACCESSION   DBNGOH010000787 DBNGOH010000000
VERSION     DBNGOH010000787.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3894)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3894)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3894
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_850961"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(29..2395)
                     /locus_tag="AC159P_15120"
     CDS             complement(29..2395)
                     /locus_tag="AC159P_15120"
                     /inference="COORDINATES: protein motif:HMM:NF014675.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HIR3696831.1"
                     /translation="MLRCMYLRAARRLLLRACLFAVSLVMCSALAAAQGQRAEYRAFW
                     VDTFNTTLNNHTDVATVVSRAKAANANAIFAQVRRRGDAWYITPLEPKFANIAAGFDP
                     LADLVNEAHANGIEVHAFVIMGAIWTSAAAPPNVTLPTSPLHAFNQHGGYDPVTKTIM
                     PGPDNWLTRTLLADGGGITFQGHRFGNDFWLDFGHPDAAAFTVDVMMHLVRNYNIDGL
                     HLDRIRYPEFAAAGQTPANGTNIGYNPTNVERFNARHDRTGTPATGDPLWMQWRRDQV
                     SNVVRRVYLNAVAEKPHLKISGALIAFGGGPACAPGPNCQTDLDAWRNAEAYWRVYQD
                     WRAWTEEGILDLAVPMNYKREHLAAQATQFAQWSNWLKDHQYDRAGLIGLGAFINPIE
                     GTLRQVRSSIFQPSPQGNHAQGVVFFSMANSNSAATNGVTATPIANPFSIPPNQVTGV
                     RVYNEFASALATGKSAGGLTLYEDPATNPEAVFSQPANIPVHPWKAAPTRGHLMGFAR
                     RADATTLDTAIVTITNLDTNDTRTTATDGGGFYGGVDLAPGPYLVKAELGADVLYTCT
                     ANVSAGSVTTADAAPETTAPASVAALAPSTPNGLGGWYTTDVTVTLDAEDDCSGVAST
                     EYSLDGGATWTPYTGAFIIDTEGTTNVLFRSSDRAGNAETPQTVTVKLDKTAPTLALS
                     ATPNVIWPPHGQTVNVTLGGDGADAVSGLASVTYLVADEYGTPLSVPFRSLSGASASW
                     ADALAVEARREGDDLDGRLYHVTATLTDAAGHTATATVDIVVPHDQRQ"
     gene            complement(2647..3579)
                     /locus_tag="AC159P_15125"
     CDS             complement(2647..3579)
                     /locus_tag="AC159P_15125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3696832.1"
                     /translation="MTPAIEIDDLTKDYEVGFLRKRKVRALDKLSLSVGRGEIFGFLG
                     ANGAGKTTTIKLLMRLMYPTSGRASILGHGIDDISMHAAIGYLPEHPYFYDHLKAREL
                     LEYCAELFGYARTERRARAADMLARVRLDEKAWDKQLRKFSKGMLQRVGLAQALVNDP
                     EVVFLDEPMSGLDPIGRREVRDLIASLRGRGTTVFLCSHILADIEVLCDRAAVMQRGR
                     LAHVGHLDELRGTGTERAMELVVAAADIARLEASLAGVEGARLTETPGGARVEVPSER
                     HVDDALAALRDAGARLVSVQPVGNPLEELFVSDK"
     gene            complement(3690..>3894)
                     /locus_tag="AC159P_15130"
     CDS             complement(3690..>3894)
                     /locus_tag="AC159P_15130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696833.1"
                     /translation="IRAALTDFKTRSSRCPANWRELTTQLRAAGLKFDAANAPLDPTG
                     VPYVLDAEACDVKLDERSEIPKK"
BASE COUNT          581 a         1147 c         1482 g          684 t
ORIGIN      
        1 ctactgccta ctgcctactg cccgctgcct actgcctctg gtcatgcggg acgacgatat
       61 cgacggtggc ggtggcggtg tgtccggcgg cgtcggtgag tgtggcggtg acgtggtaga
      121 gacgcccgtc gaggtcgtcg ccttcgcggc gggcctcgac ggcgagcgcg tcggcccacg
      181 aggcggaggc gcccgaaaga gagcggaagg ggacggagag cggcgtgccg tactcgtccg
      241 ccacgaggta ggtgacggag gcgaggccgg agacggcgtc ggcgccgtcg ccgccgagcg
      301 tgacgttgac ggtctggccg tggggtggcc agataacgtt cggggtggcc gagagtgcga
      361 gcgtgggcgc ggtcttgtcg agcttgaccg tgaccgtctg cggcgtctcg gcgttgccgg
      421 cgcggtcgga cgagcggaag aggacgttgg tcgtgccctc cgtgtcgatg atgaaggcgc
      481 cggtgtaagg agtccacgtc gcgccgccgt caagcgagta ttcggtcgag gcgacgccgg
      541 agcagtcgtc ctccgcgtcg agcgtgacgg tcacgtcggt cgtgtaccag ccgcccagtc
      601 cgttcggcgt cgagggcgcg agcgcggcga cgctcgcggg cgcggtcgtc tcgggcgcgg
      661 cgtcggcggt cgtgacgctg cccgccgaga cgttggccgt gcaggtgtag agcacgtccg
      721 cgccgagttc ggccttgaca aggtacgggc cgggggcgag atcgacgccg ccgtagaagc
      781 cgccgccgtc ggtcgccgtc gtgcgcgtgt cattggtgtc gaggttggtg atggtgacaa
      841 tcgccgtgtc gagcgtggtc gcgtcggcgc ggcgcgcgaa gcccatcagg tgcccgcgcg
      901 tcggcgcggc cttccacggg tggacgggga tgttcgcggg ttgcgagaag actgcttcgg
      961 ggttcgtcgc agggtcttcg tagagcgtca ggccgcccgc cgacttgccg gtggcgaggg
     1021 ccgaggcgaa ctcgttgtag acgcgcacgc cggtcacttg attcgggggg atggagaagg
     1081 ggttggcgat gggcgtcgcg gtgacgccgt tggtcgccgc cgagttgctg ttcgccatcg
     1141 agaagaagac gacgccctgc gcatggttgc cctgcggcga gggctggaag atggagcttc
     1201 gcacctgccg gagcgtgcct tcaatcgggt tgatgaaggc gccgaggccg atcagccctg
     1261 cgcggtcgta ctggtgatcc ttgagccagt tgctccactg cgcgaactgc gtggcctgcg
     1321 cggcgaggtg ctcgcgcttg tagttcatcg ggacggcgag gtcgagaatg ccttcttcag
     1381 tccacgcgcg ccagtcctga tagacgcgcc agtaggcttc ggcgttgcgc cacgcgtcga
     1441 ggtcggtctg gcagttcggc cccggcgcgc acgccgggcc gccgccgaag gcgatcagcg
     1501 cgccggagat tttcaggtgc ggcttctcgg cgacggcgtt cagataaacg cggcgcacga
     1561 cgttcgagac ctgatcgcgc cgccactgca tccacagcgg gtcgccggtc gcgggcgtgc
     1621 ccgtccggtc gtggcgcgcg ttgaagcgct cgacgttcgt cgggttgtag ccgatgttgg
     1681 tgccgttggc gggcgtctgc ccggcggcgg cgaactctgg atagcgtatg cggtcgaggt
     1741 gcaggccgtc gatgttgtag ttgcggacga ggtgcatcat gacatcgacg gtgaaggcgg
     1801 cggcatcggg gtggccgaag tcgagccaga agtcgttgcc gaagcggtgg ccctgaaagg
     1861 tgatgccgcc gccgtccgcg agcagagtgc gcgtgagcca gttgtccggc cccggcatga
     1921 tggttttggt gacggggtcg tagccgccgt gctggttgaa ggcgtggagc gggctggtgg
     1981 ggagcgtgac gttgggcggc gcggcggcgc tcgtccagat ggcgcccatg atgacgaagg
     2041 cgtgcacctc gatgccgttg gcgtgcgcct cgttgacgag gtcggcgagc gggtcgaagc
     2101 cggcggcgat gttggcgaac ttgggttcga gcggcgtgat gtaccacgcg tcgccgcgac
     2161 ggcgcacctg cgcgaagatg gcgttggcgt tcgcggcctt cgcgcggctg acgaccgtcg
     2221 ccacgtccgt gtggttgttg agcgtggtgt tgaaggtgtc cacccagaag gcgcggtact
     2281 cggcccgctg cccttgcgcc gccgcgaggg cggagcacat cacgagtgac acggcgaaga
     2341 gacaggcgcg cagcaagagg cggcgcgcgg cacgaagata catgcagcga agcatcgagg
     2401 attctccctg aagtaatgga aaccggcttg agctttaagg ggcagcggga cgatacgcca
     2461 aaaaccgtga ggcagtcaaa ccggacttcc ggcgccgcag aaatccttga ccgatgtttg
     2521 tgtcgaggac gagcttcaaa cacaaagaca cgaaggcaca gcttttatct tcaagctgtg
     2581 ccttcgcgtc tttgtggtta atgccttgtt gctacgcgct caactggtaa tcgaggagcg
     2641 cgcgcgtcac ttgtcgctga cgaagagttc ttcgagcggg ttgccgacgg gctggacgga
     2701 gacgaggcgc gcgcccgcgt cgcgcagggc ggcgagcgcg tcatccacgt ggcgctcgga
     2761 ggggacttcg acgcgggcgc cgccgggcgt ctccgtgagg cgcgcgcctt cgactccggc
     2821 gagcgaggct tcgaggcgcg cgatgtcggc ggcggcgacg accagttcca tcgcgcgctc
     2881 ggttccggtc ccgcgcagct cgtccagatg gccgacgtgc gcgagccgcc cgcgttgcat
     2941 gacggcggcg cggtcgcaga gcacttcgat gtcggcgagg atgtgcgagc agaggaacac
     3001 ggtcgtcccg cgcccgcgca aggaagcgat gaggtcgcgc acctcgcgcc gcccgatggg
     3061 gtcgaggccg ctcatcggct cgtcgaggaa gacgacctcg gggtcgttga cgagcgcctg
     3121 cgcgaggccg acgcgctgga gcatcccctt cgagaacttg cggagctgct tgtcccaagc
     3181 tttctcgtcg agccgcacgc gcgcgagcat gtccgccgcc cgcgctcgtc gctccgtgcg
     3241 cgcgtagccg aacagctcgg cgcagtattc cagcagctcg cgcgccttca ggtggtcgta
     3301 gaagtagggg tgctcgggca ggtagcctat cgccgcgtgc atcgagatgt cgtcgatgcc
     3361 gtggccgagg atcgaggcgc ggcccgaggt cgggtacatc agacgcatga gcagcttgat
     3421 ggtcgtcgtc ttgcccgcgc cgttcgcacc gaggaagccg aaaatctcgc cgcgcccgac
     3481 cgacaaagag agcttgtcga gcgcgcgcac cttgcgcttg cgcaggaaac cgacctcgta
     3541 gtctttcgtc aggtcgtcga tttcgatggc gggtgtcatg gcgttatctc aggaaatgtt
     3601 gaatgcggaa tgatgaatga tgaataagcg gcagcttgga tgatgagagg caccgtgcgt
     3661 ttgattcatc attccgcatt catcgttcat catttcttcg gtatctccga gcgctcgtcc
     3721 agcttcacgt cgcacgcctc ggcgtcgagc acgtagggca cgccggtcgg gtcgagcggg
     3781 gcgttcgcgg cgtcgaactt cagaccggcg gcgcggagct gggtggtcag ttcgcgccag
     3841 ttggcagggc agcgggagga gcgcgtcttg aagtctgtga gcgcggcgcg gatg
//
LOCUS       DBNGOH010000788         5542 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2868189, whole genome shotgun sequence.
ACCESSION   DBNGOH010000788 DBNGOH010000000
VERSION     DBNGOH010000788.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5542)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5542)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5542
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2868189"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(183..1196)
                     /locus_tag="AC159P_15135"
     CDS             complement(183..1196)
                     /locus_tag="AC159P_15135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696834.1"
                     /translation="MADYKDKLEEWREAARRKARELDEKYSIRERVEDSARAAGEAAR
                     RGAEKAEKVAERARAEAERFGEDFDLGERAEEVREHTKEAREKAERARAEATRAARET
                     GARVRDAGRRAGEKAGEAFGEAKRYYARAARVADVGARATRLTGASTVGLFRVYDWAR
                     ENPKQAIVVTLSLAVGVRAGAALPGLDAVFLGSHPHWFTHSALPVYALRKAGEKFDGY
                     LRERERLLEEGELETAERERVEFERKIVRYVGAPLLGAFSCAAGVAMWAQILQPGTVV
                     GAPLEWLIGGNPLLSGVYLFANGVVCFHQGYKFFMIALKDQEDAERIVREIKGLLPAV
                     ATA"
     gene            1687..2403
                     /locus_tag="AC159P_15140"
     CDS             1687..2403
                     /locus_tag="AC159P_15140"
                     /inference="COORDINATES: protein motif:HMM:NF012257.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HIR3696835.1"
                     /translation="MSHRRACDLTDRDRAEIIRRSEIGPSLDAEVLALLAAASTAVDY
                     RRRRLVYRTGDTADALFLIAEGRVKICLTEEATGREAVIDLLEEGSVFGESALYSAGA
                     AREKCAVAYENVRLVRIPVGAYQEGMAASRPLYDYTFRMVGQRLARAERRVADFALDA
                     IPARLDKLLVELSERYGVPDPDGVLIDIALPHREIASIVGSTRESITVRLNAMRRAGI
                     IDFVDRKILIKSPAAISAAV"
     gene            2500..2706
                     /locus_tag="AC159P_15145"
     CDS             2500..2706
                     /locus_tag="AC159P_15145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004581620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HIR3696836.1"
                     /translation="MKKCPTCDKPVHWQDNPFRPFCSERCKLIDFGAWVDGNYAVPAE
                     EAMPSLDELGQNQPAPGDREQREQ"
     gene            2877..3533
                     /locus_tag="AC159P_15150"
     CDS             2877..3533
                     /locus_tag="AC159P_15150"
                     /inference="COORDINATES: protein motif:HMM:NF020123.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HIR3696837.1"
                     /translation="MRLAIPARSRARALAAAALAVLLPALFFAACELDGASKKPRAPD
                     GGKSDAQLRQRAPSTSRAMPPAESAHATNSGKQGWTMLDGRRAVLEDFRGQAVVLDFY
                     ATYCPPCLEEIPHLVELQKRYGPQGLKVIGLNVGGEEDQAKVPDFVRRLDIQYQLGNP
                     DQLFVDAFFGGNSAIPQTLVFDRQGRLIKHLTGYDKEIAAQLEEAIQTALETKAEETE
                     "
     gene            3624..5063
                     /gene="pyk"
                     /locus_tag="AC159P_15155"
     CDS             3624..5063
                     /gene="pyk"
                     /locus_tag="AC159P_15155"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF004491.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HIR3696838.1"
                     /translation="MRRAKILATLGPASREPGVLESLLAAGANAVRINMSHGVQEEHA
                     ETIKRARAAAERLKRPLSVLVDLSGPKLRTGVLKGGQSVQLETSALFTITTRSVVGDA
                     REVSTNYAGIARDVQPGARILLDDGAIELLVESTTETDVITRVVNGGTLSERKGINLP
                     GIALPIPSLTDKDRRDLQFAVREGADYIALSFVRRAEDCLEAKSLIKAAGGKQPLVAK
                     IEKAAAIDHLDAIIEAADGVMVARGDLGVETSVELVPVYQKRIIERANLRGRVVITAT
                     QMLQSMINEPRPTRAEASDVANAVWDGTDAVMLSAETATGQYPVLAVATMARIVETAE
                     NGRPTGSSDITRWAGKQSGRISRALCEAAAFAAHEIGNKTIAVITESGLMAHRLSALR
                     PEQRIIALTPNSSVQNELALLWGTEAYVHPPSSSTKEMLKACERVLLEVGAAHAGETV
                     VMMAGRLSGLGLSSSVTIYTIGGEAPRDV"
     gene            5068..>5542
                     /locus_tag="AC159P_15160"
     CDS             5068..>5542
                     /locus_tag="AC159P_15160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696839.1"
                     /translation="MIGKLQISVGDLSEVLRPALFLLAALASAWVFRDARRRFDSYAA
                     ATACALLALLHPAVSLPLYLIARMYQRRDADDSTTETTGDDETDEQPSTVSSDGDETA
                     HGDAAGPGDETAHGVEAPALPRSTLAPTLVYLTAVLLVGGLYFYRDYMSFDAHFAR"
BASE COUNT          938 a         2015 c         1697 g          892 t
ORIGIN      
        1 aatttgcggg agagaagtct gcgcacacct tacaccgtgc gggctgccga aacaagaaga
       61 agaagatcgg ggcggaagcc ctgccggact cccgccccgt cttcaaagca agccgatcgt
      121 tctccgacca atctttctcc gacgccgcgc cgccttcggc ctcgtcgcac gcgtcctacg
      181 ccctacgccg tcgccaccgc cggcagtaaa cccttgattt ccctgacgat tctctccgcg
      241 tcctcctggt ctttgagggc aatcatgaag aacttgtagc cctggtggaa acagaccacg
      301 ccgttggcga agaggtagac gccggagagg agcgggttgc cgccgatgag ccattcgagc
      361 ggcgcgccga cgacagtgcc gggctgcaag atttgcgccc acatcgccac gcccgccgcg
      421 caactgaagg cgccgagcag cggcgccccg acgtagcgca cgatcttgcg ctcgaactcg
      481 acgcgctcgc gctccgccgt ctcaagctcg ccctcttcga gcagccgctc gcgctcgcgc
      541 aggtagccgt cgaacttctc gcccgccttc cgcagggcat acaccggcag cgccgagtgc
      601 gtgaaccagt gcgggtgcga gcctaagaaa acggcgtcga gtccgggcag ggccgcgccc
      661 gcgcgcacgc ccacggcgag cgagagcgtg accacgatgg cctgcttcgg gttctcgcgc
      721 gcccagtcgt agacgcggaa gaggccgacc gtcgaggcgc ccgtcagacg cgtggcgcgc
      781 gcgcccacgt ccgcgacgcg cgcggcccgc gcgtaatacc tcttcgcctc gccgaaggct
      841 tcgcccgcct tctcgcccgc gcggcggccc gcgtcgcgca cgcgcgcgcc cgtctcgcgc
      901 gccgcgcgcg tcgcctccgc gcgcgctcgc tcggccttct cgcgcgcctc cttcgtgtgc
      961 tcgcgcacct cctcggcgcg ctcgccgagg tcgaagtctt cgccgaagcg ttcggcttcg
     1021 gcgcgcgcgc gctcggccac cttctcggcc ttctcggccc cgcggcgcgc cgcctcgccc
     1081 gctgcccgcg cgctatcctc gacgcgctcg cggatgctgt acttctcgtc cagctcgcgc
     1141 gccttgcgcc gcgccgcctc gcgccactcc tcaagcttgt ccttgtaatc agccatttaa
     1201 aaacctttct gaaaagggag tcagaagtca ggagtcggaa tccagaagga aaaaccagat
     1261 gactttctat tctggattcc gactcctgac ttctgacttc tgctttacga tacgccgccc
     1321 gcgcccgcgc ggttcggccc acggcccggc gcgagagggt cggggcggct taccaatatt
     1381 attgcagaaa aacagcgctt gcgccccccg tttcttcgtg ttagagtgcc cgtcaacaag
     1441 gacggttgcc gttcgcgttc gttggtttct ttacttgtcc tcatcgaacg tgcacgaaaa
     1501 taaacctcgc gcgtcttcgg ccagaggttt tccgcactcg gtattgattc ccttttcgtt
     1561 tcacccagca caggaaccag ttccgctctc tctccgggcc gacgcccacg ctggggcgcg
     1621 caggccgcgc gcgttttcgt aagctttccg atcccgtccc tgacgcccag aaggaacccc
     1681 ccaacgatgt cacaccgacg cgcctgcgac ctgaccgacc gcgaccgcgc ggagatcata
     1741 cgccgctccg agatcggccc cagcctcgac gcggaggtgc tggccctgtt ggccgccgcc
     1801 tcgaccgccg tcgattaccg ccgccgccgc ctcgtctacc gcacgggcga cacggcggac
     1861 gcgctcttcc tgatcgccga gggccgcgtc aaaatctgcc tcaccgaaga ggccaccggg
     1921 cgcgaggccg tgatcgacct cctcgaagaa ggctcggtct tcggcgagtc ggcgctctac
     1981 tcggcgggcg cggcccgcga gaagtgcgcc gtcgcctacg agaacgtgcg cctcgtgcgg
     2041 attcccgtgg gggcttatca ggaggggatg gcggcgagcc gcccgcttta tgattacacc
     2101 ttccgcatgg tcggtcaacg gctcgcccgc gccgagcgcc gcgtcgccga cttcgccctc
     2161 gacgccatcc ccgcgcgcct cgacaagctc ctcgtcgaac tctccgagcg ctacggcgtg
     2221 cccgaccccg acggcgtgct catcgacatc gcgctcccgc accgcgagat cgcctccatc
     2281 gtcggctcca cacgcgagag catcaccgtc cgcctcaacg ccatgcgccg cgccggcatc
     2341 atcgacttcg ttgaccgcaa aatcctgatc aaaagccccg cggccatctc cgccgccgtc
     2401 tgaagactgg tgaatggtaa atagtgaata gtaaatggtg attgactggt cgaagccgct
     2461 tttgacgatt aaccatttac catttaccat ttaccaatca tgaagaagtg cccgacctgc
     2521 gacaagcccg tccactggca agacaacccc ttccgcccgt tctgctccga gcgctgcaag
     2581 ctgatcgact tcggcgcgtg ggttgacggc aactacgccg tccccgccga agaggccatg
     2641 ccctcgctcg acgaactcgg ccagaaccag cccgcccccg gcgacagaga gcagcgtgag
     2701 cagtagaagt ccggagtctg gagtctgaag tcaaaaagca tgtggcttag gactttaccc
     2761 ctcaggtatc ttctcacttc agactccaga ctccagactc cggacttctt ttactgctca
     2821 ccgctcactc ttcgtgtaaa atggccactc cctttagcga aggagtctcg tcgaagatgc
     2881 gattagccat ccccgcacgt tcgcgcgcgc gcgccctcgc cgcggcggcg ctcgccgtcc
     2941 tgctcccggc actgttcttc gcggcgtgcg agctggacgg cgcgtcgaag aagccgcgtg
     3001 cgccggacgg cggcaagtcg gacgcgcaac tgcggcagcg tgcgccgtcc acttcacgcg
     3061 cgatgccgcc cgccgagtcg gcgcacgcca cgaactccgg caagcagggc tggacgatgc
     3121 tcgacggtcg ccgcgccgtg ctcgaagact tccgcgggca ggccgtcgtg ctcgacttct
     3181 acgccaccta ctgcccgccg tgcctcgaag agattccgca tctcgtcgaa ctacagaagc
     3241 gctacgggcc gcaggggctg aaggtcatcg gtctcaacgt cggcggcgag gaggatcagg
     3301 ccaaggtgcc cgacttcgtc cggcgcctcg acatccaata ccagctcggc aaccccgacc
     3361 agttgttcgt ggatgccttc ttcggcggca actcggccat cccgcagacg ctcgtcttcg
     3421 accgtcaggg ccgactcatc aaacacctca ccggctacga caaagagatc gccgcccagc
     3481 tcgaagaagc catccagacc gcgctcgaaa cgaaggcgga ggagaccgaa taacaaccgt
     3541 gaaccgtgaa ccgtaaaccg tgacaaggaa gaaccgtctt tcacttatca cggttcacgg
     3601 ttcacggcct ttgaggttac aacttgagaa gagccaagat acttgccacg ctggggcctg
     3661 cgtcgcgcga gccgggggtg ttggagtcgc tgctggcggc gggcgccaac gccgtgcgca
     3721 tcaacatgtc gcacggcgtg caggaggagc acgcggagac catcaagcgg gcgcgggccg
     3781 ccgccgagcg gctgaagagg ccgctctcgg tgctcgtcga tctgtcgggg ccgaaactgc
     3841 ggacgggcgt gctgaagggc ggccagtccg tccagctcga gacgagcgcg ctcttcacga
     3901 tcacgacgcg ctcggtcgtg ggcgacgcgc gcgaggtctc gaccaactac gccggcatcg
     3961 cgcgcgacgt gcagcccggc gcgcgcatcc tcttggacga cggcgccatc gagctgctcg
     4021 tcgagagcac gaccgagacc gatgtcatca cgcgcgtcgt caacggcggc acgctctccg
     4081 agcgcaaggg catcaacctg ccgggcatcg cgctgcccat cccctctctg acggacaaag
     4141 accggcgcga cctccagttc gccgtccgcg agggcgccga ctacatcgcg ctctcgttcg
     4201 tgcggcgggc cgaggactgc ctcgaagcca agtcgctcat caaggccgcg ggcggcaagc
     4261 agccgctcgt cgccaagatc gagaaggccg cggccatcga ccacctcgac gcgatcatcg
     4321 aggccgcgga cggcgtgatg gtcgcgcgcg gcgacctcgg cgtcgagacc tcggtcgagc
     4381 tggtgcccgt ctaccagaag cgcatcatcg agcgcgcgaa cctgcgcggg cgcgtcgtca
     4441 tcaccgcgac gcagatgctc cagtccatga tcaacgagcc gcgcccgacg cgcgcagagg
     4501 cttccgacgt ggccaacgcc gtgtgggacg gcaccgacgc cgtgatgctc tcggcggaga
     4561 cggcgacggg tcagtacccc gtgctcgccg tggcgacgat ggcgcgcatc gtcgagacgg
     4621 cagagaacgg caggccgacc ggctccagcg acatcacgcg ctgggccggc aagcagtcgg
     4681 ggcgcatcag ccgcgcgctg tgcgaggccg ccgccttcgc cgcacacgag atcggcaaca
     4741 agaccatcgc cgtcatcacg gagtccggcc tgatggcgca tcgtctctcg gcgctcaggc
     4801 ccgagcagcg catcatcgcg ctcacgccga acagcagcgt gcagaacgag cttgcgctcc
     4861 tgtggggcac cgaggcttac gtccacccac cctcgtcgag cacgaaggag atgctcaagg
     4921 cgtgcgagcg ggtgctgtta gaggtcggcg ccgcgcatgc gggcgagacg gtcgtgatga
     4981 tggcgggccg cctctcgggc ctcggcctct caagctcggt cacgatctac acgataggcg
     5041 gcgaagcccc gcgcgacgtg tgaggctttg atcgggaaac tgcaaatctc ggtcggcgat
     5101 ctgtccgagg tgctgcgccc cgcgctcttc ctgctcgccg ccctcgcctc cgcctgggtg
     5161 ttccgcgacg cccgccgccg cttcgactcc tacgccgccg ccacggcctg cgcgctcctc
     5221 gcgctcctcc accccgccgt ctccctcccc ctctacctca tcgcgcgcat gtaccaacgc
     5281 cgcgacgccg acgattcgac gacggaaacg acgggcgacg acgagaccga cgaacagccg
     5341 tcaacggttt cgagcgacgg cgacgagacc gcgcacggcg acgcggcggg gccgggcgac
     5401 gagaccgcgc acggagtcga agcaccggcc ctcccaagat cgacgctcgc gccgaccctc
     5461 gtctacctga cggcggtgct gctcgtcggc ggcctctact tttacaggga ctacatgagc
     5521 ttcgacgcgc acttcgcgcg cg
//
LOCUS       DBNGOH010000789         3062 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1585405, whole genome shotgun sequence.
ACCESSION   DBNGOH010000789 DBNGOH010000000
VERSION     DBNGOH010000789.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3062)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3062)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3062
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1585405"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            244..798
                     /locus_tag="AC159P_15165"
     CDS             244..798
                     /locus_tag="AC159P_15165"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3696840.1"
                     /translation="MPELRRYDGAGEIPCELERQVRALLHAEWPAPEGETDGPLVAPQ
                     WHPAYFILAEGKTVWSYARTIWARVTYLGRGLKLYGLGDVVTAPALRGSGHGSRVVRE
                     ATDYIRSDREADAAMLLTGPGLEVFYRRCGWEAMPGLEVRTDEYDEHHRGGSLPMMLF
                     LSASAGRMRPGFHASPLVIPGDEW"
     gene            complement(901..1095)
                     /locus_tag="AC159P_15170"
     CDS             complement(901..1095)
                     /locus_tag="AC159P_15170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696841.1"
                     /translation="MGALWFAGSGLAMIFVGLLNLTLSRAAGRDRVVRVFCYAANLLT
                     LGFGLLFVTLDQEPQVIFGW"
     gene            complement(1229..1600)
                     /locus_tag="AC159P_15175"
     CDS             complement(1229..1600)
                     /locus_tag="AC159P_15175"
                     /inference="COORDINATES: protein motif:HMM:NF012621.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HIR3696842.1"
                     /translation="MSQRRVIAGAVTALVLAVCADAAPAATRTTTIYITGMTCAGCAI
                     SIEQVLKNTEGVEEVRVSFERGEAVVKYDDRKVTAARLREAINGIGYRATARPPKKGR
                     VAKPKATACCGAESCDTQPPG"
     gene            complement(1623..1979)
                     /locus_tag="AC159P_15180"
     CDS             complement(1623..1979)
                     /locus_tag="AC159P_15180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696843.1"
                     /translation="MLGRIKSDDVRVDVVWVPSLPADDESKVPAATAKIPDERASHYW
                     DEKGALVKAYGRVLRLGDGETAWDVYYVYGRDAEWGGEPPAPGFFMDQVGLPGGQPLD
                     GDKLAAEMRRLLRQGK"
     gene            complement(2252..2803)
                     /locus_tag="AC159P_15185"
     CDS             complement(2252..2803)
                     /locus_tag="AC159P_15185"
                     /inference="COORDINATES: protein motif:HMM:NF047645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative iron-sulfur cluster-binding
                     metallochaperone"
                     /protein_id="HIR3696844.1"
                     /translation="MSCCETGPPPNEAAPFNEETPPAPLDEAGRSCARCGSASRLVTR
                     RTVLLMLRPELLDRAGEGEYRFCADPDCRVVYFTEGGGPCFTTDDLRVRVGLKEREDP
                     VPLCYCFGFDERGAREEIVRTGQCSIPRRISALIKQDMCGCLERNPSGACCLGEVNRA
                     VKRLLAGMDEAPPVGVAVEEVVP"
     gene            complement(2809..>3062)
                     /locus_tag="AC159P_15190"
     CDS             complement(2809..>3062)
                     /locus_tag="AC159P_15190"
                     /inference="COORDINATES: protein motif:HMM:NF012621.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HIR3696845.1"
                     /translation="AQNAQPAATQPATARATFKVSGMTCAGCEETIKLALEQTPGVRA
                     SEVSYDRGEAVVEYDPIVTDIAKIQKAIDKTGYTCEIKR"
BASE COUNT          504 a         1029 c          961 g          568 t
ORIGIN      
        1 ataacggccc tcgatgtctt tccaccgagg tgtctcgatg tcgggccgcg aaggcgtcta
       61 ccgaggcgaa gtcgaagcgg cggaggtgga cgcggttcct ctcgccgtca agccacagcg
      121 agacggcccc gccgtagaga tcgaccgtca cgtccagtcg tagactctaa ttgggttcaa
      181 ctccggcttc ttcgggaatc caaaaccaag atgtaggcaa acacgctcca gactttcttc
      241 agcatgcccg agttacgacg ctacgacggc gcgggtgaaa tcccctgtga gctggagcga
      301 caggttcgcg ccctgttgca tgccgaatgg ccggcgcccg agggcgaaac ggatgggccc
      361 ctcgtcgcgc cgcagtggca ccccgcgtac ttcatcctcg ccgaagggaa aacagtgtgg
      421 agttacgcgc gcaccatctg ggcgcgggtc acatacctcg gacgaggact gaagctgtac
      481 gggttgggcg atgtcgtcac ggcccccgca ctccgcggct cggggcacgg cagccgagtc
      541 gtgcgggagg cgaccgatta catccgttcg gatcgcgagg cggacgccgc gatgctcctc
      601 acggggccgg ggctggaagt cttttatcgg cgctgcgggt gggaagccat gccggggttg
      661 gaagtgagga cggacgagta cgatgagcat catcgtggcg gcagccttcc catgatgctg
      721 tttctctcgg catccgccgg gcgcatgcgc cccggttttc atgcgtcgcc gctcgtcatc
      781 ccgggtgacg aatggtgaac gccgttaccg ttgagttgtt gaagactacg gtggattaac
      841 cggcgggatt attctgtgtt cccctgagcg tgaaggccgt caccgtcata agaacgatca
      901 tcaccatccg aagatcacct gcggttcctg gtccagcgtc acgaagagca atccaaaacc
      961 gagtgtcaga aggttggccg cgtagcagaa gactctgacg actcgatctc gcccggcggc
     1021 gcggctcagc gtgaggttga ggagtcccac gaagatcatc gccaggcccg agccggcgaa
     1081 ccacagcgcg cccatcgtga acctcccata gaacacaggc gtcagggcgg tgtgcacgac
     1141 cccgagggcg gcgagtaagg cggagctgat ttgtgcaggg tcctcagcat gtctctggtg
     1201 agaatagtac gcgccgaatc tccttacctt aaccgggcgg ctgcgtatcg caggactccg
     1261 cgccgcagca cgcggtggct ttcggcttcg ccacccggcc ctttttaggc ggcctcgccg
     1321 tcgcgcggta gccgatgccg ttaattgcct cgcgcaagcg ggcggcggtc actttcctgt
     1381 cgtcgtactt cacgacggcc tccccgcgct cgaagctcac acgcacctct tccacgcctt
     1441 ccgtgttctt cagcacctgc tcaatagaga tggcgcagcc ggcgcaggtc ataccagtaa
     1501 tatagatggt ggtggtgcgc gtggcggccg gcgccgcgtc cgcgcagacg gccagcacga
     1561 gggccgtcac cgcgccggcg atcacccgtc tctggctcat ctcattttca cctctcggat
     1621 aattacttcc cctgccggag cagtcgcctc atctcggcgg cgagcttgtc gccgtcgagc
     1681 ggctgcccac cggggagacc tacctgatcc atgaagaagc cgggcgcggg cggctcgccg
     1741 ccccactccg cgtcgcggcc gtagacgtaa tacacgtccc acgcggtctc gccgtcgccg
     1801 agcctcagca cgcgcccgta ggctttgacc agcgcgccct tctcgtccca gtaatggctc
     1861 gcgcgctcgt cggggatttt ggcggtcgcc gccggcacct tgctctcgtc gtccgccggc
     1921 agcgaaggca cccacacgac gtccacgcgc acgtcgtcgc tcttgatccg accgagcact
     1981 ttgttcttta cttcggaggc cccccgaatg acgcaggccg gtcaagtcgg cgagagtagc
     2041 gtcaccagcc gcaccttgcc cttatcgcgg ttgaaggcgg cgcgcagctc ctccaagttc
     2101 ttggagtaag ggcggggctg cggcgacggg ctcggagtcg gttcggacat tttcgcttga
     2161 cctccattcg cctgccggtt accgccagag gacgccgccg tacaggccgc caggctcagc
     2221 agaagcaggg agagccacac ccctttacgc ctcatggcac gacctcctcg acggctactc
     2281 ctacaggggg cgcttcatcc atcccggcca gaagtcgttt gaccgccctg ttcacctcgc
     2341 ccaggcagca cgcgcccgac ggattcctct cgaggcaccc acacatgtcc tgcttaatca
     2401 acgccgagat gcgccgcggg atcgagcact gcccggtgcg gacgatctct tcgcgcgcgc
     2461 cgcgctcgtc gaagccgaag cagtagcaga gtgggacggg gtcctccctt tccttcagtc
     2521 cgactctcac gcgcaggtcg tcggtcgtga agcacggccc cccgccctcc gtgaagtaca
     2581 cgacgcggca gtccgggtcg gcgcaaaacc ggtactcgcc ctcgcccgcg cggtcgagca
     2641 gctcgggcct cagcatcagc aggacggtcc tgcgcgtgac gaggcggctc gccgacccac
     2701 acctcgcgca gctgcggccg gcctcgtcca gaggggcagg gggcgtctcc tcgttgaacg
     2761 gggccgcttc attgggcggc gggcctgtct cgcagcagct catccgattt accgttttat
     2821 ctcgcacgtg tagccggtct tgtctatcgc tttctgaatc tttgcgatgt cggtgactat
     2881 agggtcgtac tcaacgaccg cctcgcctcg gtcgtagctc acttcggagg cgcggacgcc
     2941 cggcgtctgc tcaagcgcga gcttgatcgt ctcctcgcag ccggcgcagg tcataccgct
     3001 gactttgaaa gtcgcgcgcg ccgttgccgg ttgcgtagcc gcgggctgtg cgttctgggc
     3061 gc
//
LOCUS       DBNGOH010000790        10271 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1585597, whole genome shotgun sequence.
ACCESSION   DBNGOH010000790 DBNGOH010000000
VERSION     DBNGOH010000790.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 10271)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10271)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10271
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1585597"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..623)
                     /locus_tag="AC159P_15195"
     CDS             complement(<1..623)
                     /locus_tag="AC159P_15195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017315893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR3696846.1"
                     /translation="MTEHAGKRVALVTGANKGIGYETARQLAGRGLKVLIGARDERRG
                     QEAAAKLQGEGLDAHFVRLDADDATTHEAAREFIEGQFGRLDILVNNAAIALELAEKP
                     SATKMRTLRQTFDTNFFGVVSITQTLLPLIRKSDAGRVVNVSSGLASLTLHNDPGWDF
                     YQVKPLAYNASKTALNAFTVMLAHELKDTPVKVNAADPGFTATDMNG"
     gene            complement(800..1801)
                     /locus_tag="AC159P_15200"
     CDS             complement(800..1801)
                     /locus_tag="AC159P_15200"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008504481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR3696847.1"
                     /translation="MSSTTSMQPEKPLDVAADPRLSRGTKAFLKVLNSAGGPPLETLP
                     PTEAREVLVRGQASVPVDLSGIEESEKSIDADGYNLVLNVVRPAGAEGMLPVFVFTHG
                     GGWVLGDYPTHRRLVRDLVVLSGMAAVFVNYTRTPDARYPRAVNEVYAATKWVAEHGA
                     EINVDGKNLAVVGNSVGGNLAAVTAIKAKEEGGPEIKLQVLMWPVTDANFETESFKQF
                     GEKRFLTASLMKWMHDLYTTEAQRKEIHASPLQATVEQLKGLPPALIQVAESDILRDE
                     GEAYGRKLDEAGVEVTTVRYDGMIHDFGLLNGLAEEPATRSLFEHAASALKKYLRRE"
     gene            complement(1840..2343)
                     /locus_tag="AC159P_15205"
     CDS             complement(1840..2343)
                     /locus_tag="AC159P_15205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015460031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696848.1"
                     /translation="MSYVETLTLIHVVISLVGIGSGFVVVYGLLTGRRLQGWTTLFLA
                     TTVATSATGFLFPFERLLPSHKIGTVSLFVLALAILARYAFRMAGAWRWIYVVGAVVA
                     LYLNVFVGVVQAFLHVPFLHALAPTQSETPFLLTQLVVLLFFVALAVVAAIRFRVEPV
                     GMLPRTA"
     gene            2640..4316
                     /locus_tag="AC159P_15210"
     CDS             2640..4316
                     /locus_tag="AC159P_15210"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016877105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR3696849.1"
                     /translation="MARPTILTVDDDREVLRAVERDLRRKYGERYRVLKADSGQTALE
                     TLRELKVRGDAVALLLVDQRMPGMSGVEFIKQAGSLFPDAKRALLTAYADTTAAIQAI
                     NEANVHHYLLKPWDPPEQNLYPVLDDLLDDWLGDYRPPFEGIRVVGNRWSPRSHEIKD
                     FLARNQVPYQWFDVEAAATDAEVRRLTESLPEEDLKHLPLVIFLDGERLEEPTTAEVA
                     ERVGLRRRANAEFYDLAIVGGGPAGLAAAVYGASEGLRTVMIEREAPGGQAGMSSKIE
                     NYLGFPSGLSGADLTRRAVAQARRFGVEIISPQEAAGARAEGPYRFVRFADNAEISCH
                     SLLIASGVQWRTLDVPGIERLTGAGVYYGAAMTEALSCRDQEVYVVGGANSAGQAAMH
                     FAQYARRVVMLVRGDALEKSMSQYLVEQIRKTPNVHVELNSSVAEVHGAGHLEEISVH
                     CSETGETNKVPVNYLFIFIGAQPRTEWLAGLVERDERGFILTGPDLVRDGKPPRGWQL
                     ERDPYLLETSVPGIFAIGDVRKGSVKRVASGVGEGSVAISFVHQYLSKVV"
     gene            4319..5803
                     /locus_tag="AC159P_15215"
     CDS             4319..5803
                     /locus_tag="AC159P_15215"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR3696850.1"
                     /translation="MHDDTEAERDARAGGEPPTEKELLTRMEELRRVGVFKDLPEDQL
                     RWFASNSVERRFAAGEVLFARGDKPDWMSIYLEGEAQAVTDDSMSDGWVYVMRAGDPL
                     TEVSGALPYSRMTEYAAMGRAVVPTRVLLFPAALFPQMLERMPVLGKRLVGVMSDRVR
                     EATRLDVQEDKLMALGKLSAGLAHELNNPAAAARRAADELLEALEELRAANLRLCRHD
                     ISAEQRAFIDEFEQRAIAGQEDAQPLSALEQSDREDALAAWLEDRDVREGWSMAPHLV
                     EAGVEVEALGPVAAHVGEDALGDVLARVAAQLRTARLVRDIKAGTTRISELVGAIKEY
                     SYMDQAPVQEIDLHKGLDNTLLILKHKLKKKNIEVVRDYAEDLPHVTAFGSELNQVWT
                     NLIDNAIDAMPDGGRLKVRTKREPADVMVEVRDNGPGIPPEVRSRIFEPFFTTKPVGE
                     GTGLGLDTSMRIVRKHRGNLRFESKPGDTCFQVRLPLGTRATSP"
     gene            5878..6144
                     /locus_tag="AC159P_15220"
     CDS             5878..6144
                     /locus_tag="AC159P_15220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007915749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UBP-type zinc finger domain-containing protein"
                     /protein_id="HIR3696851.1"
                     /translation="MAEGCMHLERIKEVTPSADGCEDCLKSGGRWLHLRLCMECGHVG
                     CCDNSPHRHATKHFHQTSHPVIKSFEPGEEWGWCYVDELFVESL"
     gene            complement(6111..6500)
                     /locus_tag="AC159P_15225"
     CDS             complement(6111..6500)
                     /locus_tag="AC159P_15225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010485100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HIR3696852.1"
                     /translation="MRVAVGLSAAAQGRTYLAGDDGPALGAWVVGLPAVACCALLLVG
                     LLTPFACILYGLCNVFVALPWLSAPAPNPFAAGLPPLFLVIVSAALVLTGPGAFSLDA
                     RLYGRREIIIPHATRPIQSDSTKSSST"
     gene            complement(6707..7336)
                     /locus_tag="AC159P_15230"
     CDS             complement(6707..7336)
                     /locus_tag="AC159P_15230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020714224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3696853.1"
                     /translation="MNEQTSIRVFSVDDHPLLREGIAAVINSQPDMLMAAQASNGCEA
                     IQMFRQQHPDVTLMDLRLPDMSGIDALIAIRAEFPEARVIMLTTFEGDVEIQRALKAG
                     ARGYLLKSSPPKELVETIRHVHRGKKHIPQQIAANLAEHFSDETLTEREVDVLRHLSG
                     GNRNRDIAERLFISEETVKVHVKHIMEKLGASDRTQAVAIALRRGIIEL"
     gene            7638..8318
                     /locus_tag="AC159P_15235"
     CDS             7638..8318
                     /locus_tag="AC159P_15235"
                     /EC_number="3.2.2.-"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020711415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase family protein"
                     /protein_id="HIR3696854.1"
                     /translation="MMATREANGVKAGRDGLCYEPEEAVRHLSTADATLAALMERAGP
                     FAMQIRKLHDPFEALARNIVFQQLHGNAAAAIHKRVLDLFGGGKLRPADIIGAPEESL
                     RGAGLSGAKLAALKDLAAKTADGTVPTLARLRRMSDEEIVERLTRVRGIGRWTVEMLL
                     MFRLGRPDVLPVGDFAVRKGFALAYGLKESPKPKELAEYGERWRPYRSVASWYMWRAV
                     ELPPDKTR"
     gene            complement(8435..9460)
                     /locus_tag="AC159P_15240"
     CDS             complement(8435..9460)
                     /locus_tag="AC159P_15240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011529468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I phosphomannose isomerase catalytic
                     subunit"
                     /protein_id="HIR3696855.1"
                     /translation="MNLFRPRGVPPGVRVREGYITLAPELTQRLFRLEPQYRERVWGG
                     QRLKAQEPPVGEAWVAFGESVVGKGEHAGQTVAELAASYGAALLGDDAASRFGTRFPL
                     LVKLLDCADWLSVQVHPNDEQARRLVGPDASGKTEAWHLIEAEPGASILAGTKPGTTR
                     EALEAAIRGGNILEVVQRREVRAGETYLLPAGTLHSLGPGLLLYEVQQASDTTYRVYD
                     WGRPARAGRELHVEESVAVTDPEKCALLTAAPELEATRSATAVRCPYFVLDLLRISGP
                     PIKADTEGKSFHILTASAGAVDVTCGVERVRLGRYETALVAGLAGTYEINAVDGTASL
                     LRAAVPD"
     gene            complement(9457..10215)
                     /gene="kduD"
                     /locus_tag="AC159P_15245"
     CDS             complement(9457..10215)
                     /gene="kduD"
                     /locus_tag="AC159P_15245"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019184096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HIR3696856.1"
                     /translation="MLDTFRLDGKVALVTGASAGLGAAIAVALAEAGADVAAHGNSRS
                     PDATCESIKSAGRRCLALSADLADRETPRRLVETTLEGFGRLDILVNNAGIIRRAPAV
                     EFSEEDWASVIEVNLSAVFRLSQLVGRHMIERGSGKVVNIASLLSFQGGVTVPAYAAS
                     KGGVAQLTKALANEWAAKGINVNAVAPGYMRTDNTAALQKDETRNRQILERIPAGRWG
                     EPRDLTGAVVFLASSASDYVNGHVLVVDGGWMGR"
BASE COUNT         1757 a         3335 c         3435 g         1744 t
ORIGIN      
        1 tgcccgttca tgtcagtcgc ggtgaaaccg gggtctgccg cgttgacctt gaccggcgtg
       61 tccttcaatt cgtgcgccag catcaccgtg aaggcgttga gcgcggtctt cgaagcgttg
      121 taggcgagcg gcttcacctg ataaaagtcc cagcccggat cgttgtgcag cgtcagcgag
      181 gcgagaccgc tggagacgtt cacgacgcgc cccgcgtcgg atttgcggat aagcggcagc
      241 agggtttgcg tgatcgagac gaccccgaaa aagttggtgt cgaacgtctg ccgcagcgtc
      301 cgcattttcg tcgcgctcgg cttctccgcc agttcgagcg cgatggccgc gttgttgacg
      361 aggatgtcga gcctgccgaa ctggccttcg ataaactcgc gcgccgcctc gtgcgtcgtc
      421 gcgtcgtcgg cgtcgagacg cacgaaatgt gcgtccaagc cttcgccttg caacttcgcc
      481 gcggcctctt gcccgcgccg ctcgtcgcgc gcgccgatca gaactttcaa gccccgtccg
      541 gcgagttgtc gcgccgtctc gtaaccgatg cctttgttcg cccccgtcac gagcgcgact
      601 cttttccccg cgtgctcagt cattccatct ccctcttgaa gtattcgttc atgttcaggc
      661 gaggccgctg tcggcgcgca atgtctgccc gttgaaccag ccgccgtctt gccaccttct
      721 cttccctacc ctacgtcacg tcttcccgct ccttttcacc tgtgcgttca tgccggcggt
      781 cgccctgtcg ccctgcgcct cactcccgcc gcaagtattt cttcagcgcc gaggccgcgt
      841 gttcgaacag cgagcgggtc gcaggttctt ccgccagacc gttgagcagg ccgaagtcgt
      901 gaatcatgcc gtcgtaacgc accgtcgtca cctcgacgcc ggcctcgtcc aacttgcgcc
      961 cgtaggcttc gccctcgtcg cgcaggatgt cgctctccgc gacctgtatc agcgcggggg
     1021 gcagcccctt cagttgctcg accgtggcct gcaagggcga ggcgtggatt tccttgcgct
     1081 gggcttcggt cgtgtagagg tcgtgcatcc acttcatcag cgacgcggtg aggaaacgct
     1141 tctcgccgaa ctgcttgaag gactcggtct cgaaattcgc gtccgtcacc ggccacatca
     1201 acacctgaag cttgatctcc gggccgccct cctctttggc cttgatggcg gtgacggcgg
     1261 ccagattgcc cccgacgctg ttgcccacga cggccagatt cttcccgtca acgttaatct
     1321 ccgcgccgtg ctccgcgacc cacttcgtcg ccgcgtagac ttcgttgacc gcccgtgggt
     1381 aacgtgcgtc gggcgtgcgc gtgtagttga cgaagacggc ggccatcccc gaaaggacga
     1441 cgaggtcgcg caccagacgc ctgtgcgtcg ggtagtcgcc gagcacccag ccgccgccgt
     1501 gcgtgaagac gaagacggga agcatccctt cggcgcccgc ggggcggacg acattcagca
     1561 cgaggttgta accgtctgcg tcgatggact tctcagactc ctcgatgccg gaaagatcga
     1621 ccgggacgga agcctgcccc cggacgagca cctcgcgagc ctcggtcggc ggcagggttt
     1681 cgagcggcgg gccgcccgcc gagttgagca ctttcaaaaa cgccttcgtc ccgcgcgaca
     1741 gtcgcgggtc ggcggccacg tccaatggtt tttcgggctg catagatgtc gtgctactca
     1801 tgacctctcc ttttccatga ccttgaattt gctcgccgct caggccgtgc gcggcagcat
     1861 cccgaccggc tcgacgcgga acctgatcgc ggcgacgacg gcgagcgcga cgaagaacaa
     1921 caggacgacg agctgcgtga gcaggaacgg cgtctcggac tgcgtcgggg ccagcgcgtg
     1981 caaaaacggc acatgcagga acgcctgcac caccccgacg aaaacgttca ggtagagggc
     2041 gaccaccgcg ccgaccacgt aaatccaacg ccacgcgccg gccatacgga aggcgtagcg
     2101 cgcgaggatc gccaacgcca gcacgaacag cgagacggtg ccgatcttgt gcgacggcaa
     2161 cagccgttcg aacgggaaga gaaagcccgt cgcactcgtc gccaccgtcg tcgccagaaa
     2221 gagcgtcgtc caaccttgaa gccgtctccc ggtcaacagc ccgtacacga cgacgaaccc
     2281 cgagccgatc cccaccaggc tgatgacgac gtggatcaac gttaaagtct ctacgtagct
     2341 catgatgtgc ttcctttcgg aatgggtttg acgttcgaat cctttgcgtg gtgaccatct
     2401 tgaagcgcca tactatgcag caacggggcg cgtcgaacat cacgggatag ggtgtttttt
     2461 cgctaccacg gtgggagagt gcaagaatac caccatcgtg gtagccggtc ggcaccgagc
     2521 ctgtagtatc tttaacgcat aaaacggacg gttgaatgcg agcggctttc cgcggccccg
     2581 gcgcgtccga cgagtaggtt ggccgtgacg gctttaatcg aactgcttga gaaatcgaga
     2641 tggcgagacc aacgatcctg acggtggacg acgaccgcga ggtgttgcgc gcggtcgagc
     2701 gcgatctgcg gcgcaagtac ggcgagcgct accgcgtgct caaggccgac tccggccaga
     2761 ccgcgctcga aaccctgcgc gagctgaagg tgcgcggcga cgcggtcgcg ctcctgctgg
     2821 tcgatcagcg catgccgggg atgtccggcg tcgagttcat caagcaggcc ggctccctct
     2881 tccccgacgc caagcgcgcg ctgctgaccg cctacgcgga cacgaccgcg gcgattcagg
     2941 ccatcaacga ggccaacgtc caccactatc ttctgaagcc gtgggacccg cccgagcaga
     3001 acctctatcc cgttctggac gacctgctcg acgactggct gggggactac cgcccccctt
     3061 tcgagggcat ccgcgtggtc ggcaatcgct ggtcgccgcg ctcgcacgag atcaaagatt
     3121 ttctcgcgcg caatcaggtg ccctaccagt ggttcgacgt ggaggccgcc gcgacggacg
     3181 cagaggtgcg ccgtctgacc gagtctctgc cggaagaaga tctgaagcac ttgccgctcg
     3241 tcatcttcct cgacggcgag cggctcgaag agccgacgac cgcggaggtc gccgagcgcg
     3301 tgggtttgcg ccgccgcgcg aacgcggagt tttacgatct ggcgattgtc ggcggcggcc
     3361 cggcggggtt ggccgccgcc gtgtacggcg cgagcgaggg gctacggacg gtgatgatcg
     3421 agcgcgaggc gccgggcggt caggccggga tgagttcgaa gatcgagaac tacctcgggt
     3481 ttccttcggg gctgtcgggc gccgacctga cgcgccgcgc ggtggcgcag gcgcgtcgct
     3541 tcggcgtcga gatcatctcg ccgcaggagg ccgcgggcgc gcgcgccgaa gggccgtacc
     3601 gcttcgtgcg cttcgccgac aacgcggaga tcagttgcca ctcgctgctg atcgcgagcg
     3661 gcgtgcagtg gcggacactc gacgtgcccg gcatcgagcg cctgacgggt gcgggcgtct
     3721 actacggcgc ggcgatgacc gaggcgctgt cgtgccgcga ccaggaagtc tatgtggtgg
     3781 gcggtgcgaa ctcggcggga caggcggcga tgcactttgc gcagtacgca cggcgggtcg
     3841 tgatgctcgt gcgcggtgac gcgctcgaaa aatccatgtc gcaatacctc gtcgagcaga
     3901 tcaggaagac gcccaacgtc cacgtcgagc tgaactcgtc ggtcgcagag gttcacggcg
     3961 cgggccacct cgaagagatt tccgtccact gctccgagac aggcgagacc aacaaggtgc
     4021 ctgtaaacta cctcttcatc ttcatcggcg cgcagccgcg caccgaatgg ctcgcgggct
     4081 tggtcgaacg cgacgagcgc ggtttcatcc tgaccggccc cgacctcgtg cgcgacggca
     4141 agcccccgcg cgggtggcag cttgagcgcg acccgtactt gttggagacg agcgtgcccg
     4201 gcatcttcgc catcggcgac gtgcgcaaag gctcggtcaa gcgcgtcgcg tcgggcgtgg
     4261 gcgaaggctc ggtcgccatc tctttcgttc accagtacct gagcaaagtg gtgtgaccat
     4321 gcacgacgac acggaggcag agagggacgc gcgcgcgggc ggcgagccgc cgaccgaaaa
     4381 agagctgctt acgcggatgg aggagctgcg ccgcgtcggc gttttcaagg acctgccgga
     4441 agaccagctc cgttggttcg catcgaactc ggtggagcgg cggttcgcgg cgggcgaagt
     4501 cctcttcgcg cgcggcgaca agccggactg gatgagtatc tacctcgaag gggaggcgca
     4561 ggccgtgacc gacgacagca tgtcggacgg ctgggtctac gtcatgcgtg cgggcgaccc
     4621 cctcacggaa gtctccggcg cgctccccta ctcgcgcatg acggagtacg ccgccatggg
     4681 gcgcgccgtc gtcccgacgc gcgtgctgct cttccccgcc gcgctcttcc cgcagatgct
     4741 cgaacggatg ccggtgctcg gcaagcggct ggtcggcgtg atgagcgacc gcgtgcgcga
     4801 ggcgacgcgg ctggacgtgc aggaagacaa gctgatggcg ctcggcaaac tctcggcggg
     4861 cctcgcgcac gagctgaaca accctgcggc ggcggcccgc cgcgcggcgg acgaactgct
     4921 cgaagcgctc gaagagctgc gcgccgccaa cctgcgtttg tgccgccacg acatctccgc
     4981 cgagcagcgc gctttcatcg acgagttcga gcagcgggcc atcgcggggc aggaggacgc
     5041 gcagcccctc tccgctctgg agcagagcga cagggaggac gcgctcgccg cgtggctcga
     5101 agaccgggac gtgcgcgaag ggtggagcat ggccccgcat ctggtggaag ccggcgtgga
     5161 agtcgaagcg ctcgggccgg tcgcggctca cgtcggcgag gatgcactgg gcgacgtgct
     5221 cgcgcgcgtc gcggcgcaac tgcgcacggc gcggctggtg agagacatca aggcgggcac
     5281 cacgcgcatc tccgaattgg tcggcgcgat caaagagtat tcctacatgg atcaggctcc
     5341 cgtgcaggag atcgacctgc acaaggggtt ggataacacg ctgctcatcc tcaagcacaa
     5401 gctgaagaag aagaacatcg aggtcgtccg ggactacgcg gaagatttgc cgcacgtcac
     5461 cgccttcggc agcgagctga atcaggtgtg gacgaacctc atcgacaacg cgatcgacgc
     5521 gatgccggac ggcgggaggc tcaaagtccg caccaagcgc gagcctgcgg acgtgatggt
     5581 cgaggtgcgc gacaacggcc ccggcatccc gcccgaagtg cgctcgcgca tcttcgagcc
     5641 tttcttcacc accaagcccg tcggcgaagg cacggggctg ggcctcgaca cttccatgcg
     5701 catcgtccgc aagcaccgcg gcaacctgag gttcgagtcg aaacccggcg acacctgctt
     5761 tcaggtgcgc ctcccgctcg gcacgagggc gacgagtcct tgaagagggc gggcgaagcg
     5821 tgagagcgaa gttgacgaaa gtctttgagc gggcgcttga cttgaaggga gggctggatg
     5881 gcggaaggct gcatgcatct ggagcgaatc aaagaggtga cgccgagcgc ggacggctgc
     5941 gaagactgtc tgaagagtgg cggtcgctgg ctgcacctgc gcctctgcat ggagtgcggc
     6001 cacgtcggct gttgcgacaa ctcgccccac cgccacgcga ctaaacattt ccaccagacg
     6061 agtcaccccg tcatcaagtc gttcgaaccc ggcgaggagt ggggctggtg ctacgtcgat
     6121 gagcttttcg tcgagtcgct ctgaatcgga cgtgtggcgt gagggatgat gatttcgcgg
     6181 cgaccgtaga gacgcgcgtc gagcgaaaac gcccccggcc ccgtcagcac gagtgcggcg
     6241 gagacgatga ccaagaagag cggcggcaga cccgccgcga acggattcgg ggcgggcgcc
     6301 gacagccacg gaagcgcgac gaacacgttg cacagcccgt acaggatgca cgcgaacggg
     6361 gtcagcagcc cgaccagcag taaggcacag cacgcgaccg ccggcaggcc gacaacccac
     6421 gccccgagcg cagggccgtc gtcacctgcc agataggtcc ggccttgagc cgccgccgac
     6481 agacccaccg ccacacgcat cagcagcaac ccgacgccgg ccctgccgtc cggaaagctt
     6541 aatgaataga gtctgtgcag cccgcgcctc cctcgtcttc gccggtgctg gtgggacaca
     6601 cccgacggta gggtaacggc tgcgcccccg cggcgaatcc ctaatgcgcg gaatctttta
     6661 atgccactat cgtggtatag acggggtttc ggacggaggg cgccgctcac aactcgatga
     6721 tgccgcggcg cagggcgatg gcgaccgcct gcgtgcggtc gctggccccg agtttctcca
     6781 tgatgtgctt gacgtgaacc ttgacggtct cttccgagat gaagaggcgt tccgcgatgt
     6841 cgcggttgcg gttcccgccg gagaggtggc gaagcacgtc cacctcgcgc tcggtcaggg
     6901 tctcgtcgct gaagtgctcg gccaggttcg cggcgatctg ttgggggatg tgcttcttcc
     6961 ccctgtggac gtggcggatc gtctcgacca gctctttggg ggggctgctc ttgagcaggt
     7021 agccgcgcgc ccccgccttc agggcgcgct ggatttccac gtcgccctcg aaggtggtca
     7081 gcatgatgac gcgggcttcg gggaactcgg cgcggatggc gatcaaagcg tcgatgccgc
     7141 tcatgtccgg gagccttaaa tccatgagcg tcacgtccgg atgttgttgg cggaacatct
     7201 ggatggcctc gcagccgttg gacgcctgtg cggccatcag catgtccggc tggctgttga
     7261 tgacggcggc gatcccttcg cgcagcagcg ggtggtcgtc tacactgaag acgcggatgc
     7321 ttgtctgttc gttcattgat ctccctcgct tcctgaatgc gaggacttgc tcttccgtcg
     7381 ttcggccgag gctccactgt ccgcaggcgc acatcatagc gccgccgccg agccgtcgtc
     7441 cacgggcgtg gtttaccgcg cgggacggaa cggaagagcg cgccgccgcg aatacgccgc
     7501 atcctgtgca agcaacattg tttcaggcag gcaatttatt tagaatcggc ccgcatggaa
     7561 aagaatccgc ggctgccgat tgttgcgagc acgacggcgt gcaggtgttc ggcggcgcgc
     7621 tttgaagaag gcggtcgatg atggcgacgc gagaggcgaa cggtgtgaag gcggggcgcg
     7681 acggcttgtg ttacgagccg gaggaggcgg tgcggcatct ctctacagcc gacgcgacgc
     7741 tggcggcgtt gatggagcgg gccgggcctt tcgcgatgca gatccgcaag ctgcacgacc
     7801 ctttcgaggc gctcgcgcgg aacatcgtct tccagcagct acacggcaac gcggcggcgg
     7861 cgatccacaa gcgtgtgctc gacctcttcg gcggcggcaa gctccgcccc gcggacatca
     7921 tcggcgcgcc ggaggagagc ttgcgcggcg cgggtctttc gggcgcgaag ctggcggcgc
     7981 tgaaagacct cgcggcgaag accgcggacg gcaccgtgcc gacgcttgcg cggctgcggc
     8041 ggatgtcgga cgaggagatc gtcgagcgcc tgacgcgtgt gcgcggcatc ggtcggtgga
     8101 cggtggagat gctgctgatg ttcaggctcg gcaggcccga cgtgttgccg gtcggcgact
     8161 tcgccgtgcg caaaggtttc gcgctcgcct acgggctgaa ggagtcgccg aagccgaagg
     8221 agcttgcgga gtacggcgag cgttggcggc cctacagatc cgtggcgagc tggtacatgt
     8281 ggcgggccgt cgagctaccg cccgacaaaa cgcgttgaca cttcgaccgt ctgacaagat
     8341 taaccacgaa ggcacgaagg actcaaagaa gagttagaga tcgaacgttc gttcattctc
     8401 tgactcttct ttgagtcctt acgtgccttc gtggttaatc cggcacggcg gctcggagta
     8461 acgacgccgt tccgtctacg gcattgattt cgtatgtgcc ggcgagtccc gcgacgaggg
     8521 ccgtctcgta tctgccgagt cgcacgcgct cgacgccaca ggtgacatcg acggcacccg
     8581 cggaagccgt gaggatgtgg aaggacttcc cttccgtgtc ggccttgatg ggagggccgc
     8641 tgatccgcaa caggtcgagg acgaaatagg ggcagcgcac agcggtcgcg gatcgagtcg
     8701 cttcgagttc gggcgcggcg gtcagcagcg cgcacttctc cgggtctgtc acggcgaccg
     8761 attcttcgac gtgcaactcc ctgcccgcac gcgccggtcg cccccagtcg tagacgcggt
     8821 aggtcgtgtc gctcgcctgc tgcacctcgt acaagagcag acccggcccc agagagtgca
     8881 gcgtccccgc gggcagcagg taggtctcgc ccgcgcgcac ctcgcgccgc tgcacgacct
     8941 caaggatgtt gccccccctg atggccgcct caagtgcttc gcgcgtcgtg ccgggcttcg
     9001 tgcccgcgag gatcgacgcg cccggctcgg cctcgatgag gtgccacgcc tcggtcttcc
     9061 ccgacgcgtc cggcccgacg agtcgccgcg cctgctcgtc gttcgggtgc acctgcaccg
     9121 agagccagtc ggcgcagtcg agcagcttga cgagcagtgg gaagcgcgtg ccgaagcggg
     9181 aggccgcgtc gtcgccgagc agcgcggcgc cgtaactcgc cgcgagttcg gcgactgtct
     9241 ggccggcgtg ctcgcctttg ccgacgacgc tctcgccgaa ggcgacccac gcctcgccga
     9301 cgggcggttc ctgcgccttc agccgctggc cgccccacac gcgctcgcgg tactgcggct
     9361 caagccggaa gagtctctgc gtcaattcgg gagccaacgt gatgtaaccc tcgcgcacac
     9421 gaacgcccgg cggcacgccg cgcgggcgga atagattcaa cgccccatcc acccgccgtc
     9481 aacgacgagc acgtggccgt tgacgtagtc gctcgccgaa gaagcgagga agacgaccgc
     9541 acccgtcaag tcgcgcggct cgccccagcg ccccgccggg atgcgttcga gtatctgccg
     9601 gttgcgcgtc tcgtcttttt gcagcgcggc ggtgttgtcg gttctcatgt agccgggcgc
     9661 gacggcgttg acgttgatcc ctttcgccgc ccactcgttc gccagcgcct tcgtgagctg
     9721 ggccacgccg cccttcgagg ccgcgtaggc aggcacggtc acgccgccct gaaacgaaag
     9781 cagcgaggcg atgttgacga ccttgcctga gccgcgctcg atcatgtgtc tgcccacgag
     9841 ctgagagagg cggaagaccg ccgacaggtt gacttcgatg acggaggccc agtcttcctc
     9901 ggaaaactcg acggcggggg cgcggcggat gatgcccgca ttgttgacga ggatgtcgag
     9961 ccgaccgaac ccttcgagcg tcgtctcgac cagacggcgc ggcgtctcgc ggtcggcgag
    10021 gtcggctgaa agggcgagac atcggcgtcc cgcactcttg atcgactcgc aagtcgcatc
    10081 cgggctgcgc gagttgccgt gggccgccac gtcggcgccc gcttcggcga gcgcaacggc
    10141 gatggccgcg ccgagtccgg cggacgcgcc cgtcaccaaa gccaccttcc cgtcgagcct
    10201 gaaagtatcg agaatcaagt tacacactcc ttcacggatg ggcgaagtca gcggtattcc
    10261 gttgcaaaac c
//
LOCUS       DBNGOH010000791         2852 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2685684, whole genome shotgun sequence.
ACCESSION   DBNGOH010000791 DBNGOH010000000
VERSION     DBNGOH010000791.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2852)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2852)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2852
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2685684"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..398
                     /locus_tag="AC159P_15250"
     CDS             <1..398
                     /locus_tag="AC159P_15250"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HIR3696857.1"
                     /translation="FLLRHDAVRGESMGTVGFCMGGVLSLYAASKNARVGACVVFYGI
                     HPKVEPDLEALRAPVLGIYAEKDQFVPPAAARSLEERLQALGREAEFHVYPGTDHAFF
                     NDTRPEVHDAQAAADAWRRTLEFLRRHLG"
     gene            complement(494..1708)
                     /locus_tag="AC159P_15255"
     CDS             complement(494..1708)
                     /locus_tag="AC159P_15255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696858.1"
                     /translation="MRQLIKFCLLITAALALNVVLSATQLAEAQLIISEFRVRGPNGA
                     NDEFIELYNNSGADHTVTSAMPSTGYAVAASNGVARCVVPNGTVIPNRGHYLCVNSVG
                     YSLASYPAGNGTTATGDATYTTDIPDNAGIAIFNTSVAANFVLANRMDAVGSTSEANT
                     LYKEGTGYPALTPFSIDYSFYRDNCGKSGSITTFGACPISTPKDTNNNAADFVFVDTN
                     GTSAGAGQRLGAPGPENLSSPIQRNASFGIALLDPCVAGSSPPNRVRDFTSNPAQNST
                     FGTLDIRRTYTNATGGNVTRLRYRVIDLTTFPAPSGIADLRPRTSTAVVVTVDRPPCG
                     SGTSNVTVQGTTLEQPPSQPNGGGFNSSMSAGTVTLATPLANGASIDLRFLLGIQQNG
                     SFKFFINVEALP"
     gene            2017..>2852
                     /gene="ttcA"
                     /locus_tag="AC159P_15260"
     CDS             2017..>2852
                     /gene="ttcA"
                     /locus_tag="AC159P_15260"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014404431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HIR3696859.1"
                     /translation="MFVESKIKTKLLRQMGEAIKDFGMIAEGDRVMVCLSGGKDSYTM
                     LDLLLDVQKRAPVNFDILAVNLDQKQPNFPAHVLPEYLEARGVPFRIVERDTYSTVKR
                     LVPEGKTYCSVCARLRRGLLYQTAVEEGCTKVALGHHADDIITTFLLNIFYVGTLKAM
                     PPVLRSDDRRNTVIRPLAYCRERDIAEYAAEKEFPIIPCDLCGSQTDLKRVRMKRLVA
                     DLEREIPHVRASMLTALGNVAPSHLLDPSLFDFKNLRAATGDVEAELDSALGHTDVAD
                     APA"
BASE COUNT          485 a          928 c          973 g          466 t
ORIGIN      
        1 agtttctgct caggcacgac gcggtcagag gcgagagcat gggcacggtg ggcttctgca
       61 tgggcggggt gctgtcgctc tacgccgcct cgaagaacgc gcgggtcggc gcgtgcgtcg
      121 tcttctacgg catccacccg aaggtcgagc cggacctcga agccttgcgc gcgcccgtgc
      181 tcggcatcta cgcggagaag gatcagttcg tgccgcccgc ggcggcgcgc tcgctcgaag
      241 agaggttgca ggcgctcggc agggaggcgg agttccacgt ctaccccggc acggatcacg
      301 ccttcttcaa cgacacgcgc cccgaagtcc acgacgcgca ggccgccgcc gacgcctggc
      361 ggcgcacgct cgaattcctg cgccgtcatc tcggctgaaa taagacgacg gggaagagtg
      421 gaaaagggga aagggcgaaa agggttttca tccctcttcg ccctttcccc ttttccactc
      481 ttcccctctc cgtctatggc agcgcctcga cgttgatgaa gaacttgaac gagccgttct
      541 gctggatacc gagcaggaac cgcaggtcaa tcgacgcgcc gttcgccaaa ggcgtcgcca
      601 gcgtcaccgt ccccgccgac atgctgctgt tgaacccgcc gccgttcggc tgcgagggcg
      661 gctgctcaag ggtcgtcccc tgcaccgtca cgttcgaggt gcccgacccg cacggcgggc
      721 gatccaccgt caccaccacc gccgtcgagg tgcgcgggcg caagtccgcg atgcccgacg
      781 gcgccgggaa cgtcgtcagg tcgatgacgc ggtagcgcag gcgcgtgacg ttgccgccgg
      841 tagcgttggt gtaggtgcgg cggatgtcga gcgtcccgaa cgtcgagttc tgggccgggt
      901 tggaggtgaa gtcgcggacg cggttgggcg gcgaggagcc ggccacgcac gggtcgagca
      961 gcgcgatgcc gaaggaggcg ttgcgctgga tgggcgacga aaggttctcg gggccggggg
     1021 cgccgaggcg ctggccggcg cccgcgctgg tgccgttggt gtccacgaag acgaagtccg
     1081 ccgcgttgtt gttcgtgtcc ttcggcgtgc tgatggggca ggcgccgaag gtggtgatcg
     1141 agccgctctt gccgcagttg tcgcggtaga acgagtagtc gatggagaaa ggcgtcagcg
     1201 ccgggtagcc cgtcccttcc ttgtagagtg tattcgcctc cgaggtcgag ccgaccgcgt
     1261 ccatacggtt ggcgagcacg aagttcgcgg cgaccgaggt gttgaagata gcgatgccgg
     1321 cattatcggg gatgtcggtg gtgtaggtgg cgtcgccggt ggcggtggtg ccgttgcccg
     1381 ccgggtacga agcgagcgag tagccgaccg agttgacgca caggtagtgg ccgcggttcg
     1441 gaatcaccgt gccgttcggg acgacgcagc gcgcgacgcc gttcgaggcc gccacggcgt
     1501 agcccgtgct gggcatggcg ctcgtaaccg tgtggtcggc gcccgaattg ttgtaaagct
     1561 cgatgaactc gtcgttggcg ccgttcggcc cgcgcacgcg gaactcgctg atgatgagct
     1621 gcgcctcggc gagttgggtt gcggaaagca cgacgttgag cgccagcgcg gcggtaatca
     1681 acaagcagaa ctttatcaat tgtcgcatcc ctagtttctc ctgacggtgt ggaaaccaat
     1741 cacgaattgc tccggcggct gccggggcca gcctgaaaac gcgaggtgaa aatttgtgtt
     1801 gaagaacaag tgggcgctaa tcctagcaca attcttcgga cgcgggtgac aaaattcggc
     1861 ggcgcgcggc ggcggcccga acaccccgcc gcccggccac cgccggttgc atatccggcc
     1921 cacgtgcctt aaaacatgcg aagcgaatga cgcgcggcgc accgcccgtc gagtaaggag
     1981 tgacggcgcg cgccggcgcg gggggtagat acagggatgt tcgtcgagag caagatcaag
     2041 accaagctgc tgcgccagat gggcgaggcc atcaaggact tcgggatgat cgcggaaggc
     2101 gaccgcgtga tggtctgcct gtcggggggc aaggacagct acacgatgct cgacctgctg
     2161 ctcgatgtgc agaagcgcgc gcccgtcaat ttcgacatcc tcgcggtcaa cctcgaccag
     2221 aagcagccga acttccccgc gcacgtcctg cccgaatatc ttgaggcgcg cggcgtcccg
     2281 ttccgcatcg tcgagcgcga cacctactcg accgtcaagc ggctcgtgcc cgagggcaag
     2341 acctactgct cggtgtgcgc gcgcctgcgc cgcggcctcc tctaccagac ggcggtcgag
     2401 gagggctgca ccaaggtcgc gctcggccac cacgccgacg acatcatcac cacgttcctg
     2461 ctgaacatct tctacgtcgg gacgctcaag gcgatgccgc ccgtcctgcg ctcggacgac
     2521 cggcgcaaca cggtcatccg cccgctcgcc tactgccgcg agcgcgacat cgccgagtac
     2581 gccgccgaga aggagttccc catcatcccg tgcgacctct gcggctcgca gaccgacctc
     2641 aagcgcgtgc gcatgaagcg gctggtggcc gacctcgaac gcgagatacc gcacgtccgc
     2701 gcctcgatgc tgacggcgct cggcaacgtc gcccccagcc acctgctcga cccctcgctc
     2761 ttcgacttca agaacctgcg cgcggccacc ggcgacgtgg aggccgaact cgactcggcc
     2821 ctcggccaca ccgacgtcgc ggacgcgccc gc
//
LOCUS       DBNGOH010000792         4186 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4701376, whole genome shotgun sequence.
ACCESSION   DBNGOH010000792 DBNGOH010000000
VERSION     DBNGOH010000792.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4186)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4186)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4186
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4701376"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(110..424)
                     /locus_tag="AC159P_15265"
     CDS             complement(110..424)
                     /locus_tag="AC159P_15265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696860.1"
                     /translation="MNDRARKRAALKRRLARAGALRLAKRLLKPLPLVGTAMTVGLAG
                     HEIRKKGALRGAAHVGLDLLPVVGTAKAVVEMFTGDLIPDKKPRRVPESLRRGGKDKT
                     DK"
     gene            complement(553..1422)
                     /locus_tag="AC159P_15270"
     CDS             complement(553..1422)
                     /locus_tag="AC159P_15270"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010994942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3696861.1"
                     /translation="MSAGGETSSGGGELFELLEAGFPGIGWGARLARMEALGVEWERV
                     SKPFVRYEAGRAVSHVGVMPLPLVVAGERVNAGAIHAVCTRAEARRRGHYRATLEDAL
                     RYCDARFETLVLTTAQPELYEPFGFRHVGEHRFGLAVGSEGARGGFRELRPAEAEDLS
                     ILRRLLEGRPAVSRVVGVEGEVAVFAFNETFRPLLYADELDVIVSAEFEGATLHLFDV
                     VGRAACAVGDILERAPRLVERVVCYFSPDLLAGAEGALPEPHLLDGDDHLMVRGPFAA
                     AGRAFMLPRTARC"
     gene            complement(1419..1904)
                     /locus_tag="AC159P_15275"
     CDS             complement(1419..1904)
                     /locus_tag="AC159P_15275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696862.1"
                     /translation="MFTVRAAFNEQAEWRAAAEAARAFFAELRNFAELMPGVERVTAE
                     AGGAAARWLIRADVPVVGSIRQAFAVAQAVDLPGLIEWLPAAGERKNFLRYEVSFEER
                     GAMTRVGVRLAVEVRRENARELHPLAGLAGERRLSAELEKGITHMMRTYLQKARARLE
                     R"
     gene            complement(2036..>4186)
                     /locus_tag="AC159P_15280"
     CDS             complement(2036..>4186)
                     /locus_tag="AC159P_15280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HIR3696863.1"
                     /translation="ADHHGQPSQFFFPPAALPVNPNLPPEQTERWLAAFGRANAAQFD
                     QRQWDFYVRDIFDLFYPGYWDSWPALNGATGMTYETDSGGWKGLRWRRDDDTIVTMRS
                     GIAKHFVASLTTLATAASNREARVRDYYEFKRTAVEEGRSGKIRRFVILPGRNPGHAA
                     ELVENLVRAGIEVQQAIASFRSARAHDYSSQNSPATSRDFPAGAYVVDLAQPQKRLAK
                     ALLEMHTPLDPAFAREQLARFERNERRSSNKEGYGFYDITSWSLPLVFGVEAYWTEDA
                     AAVSGTPVSMVEFTTRVGGASFPAPGGNALVSTASVPGGVTGRAQVAYIIPYESNGAA
                     SLVYRLLREDYKLAVATRTLSAGGREWPRGTVVVRVSRNPDTLHERIARIAQEAGVTA
                     TAVNTGFTETGDVSVGAETVVSLRKPKIIVVADEPVSTTGYGAMWWTFDRMGVEFTPM
                     TIQAVKSTRLDDYNVIILPDGSAGGYFAQFGKPGVDALRAWVQRGGTLVLVKGAAVFG
                     AIKDVNLTSSRLVGSDDDDQKPSGDAAPTPTPSPTPTPADEQSAARRGRQQPAASTPE
                     QQQISSAQTEKMEGAAPDLPPIASPSARPGRVPEAVPGAIMKATLDRTTPLTYGYEAT
                     TLPVLVDSGYFFRPSKEGTNALIFSDDAKQPLYLAGFVWPTTEKLLRGTAYVIDEPTG
                     KGHVVLYAEDPNFRAIWRSTTRLFFNSFLFQPVL"
BASE COUNT          679 a         1459 c         1390 g          658 t
ORIGIN      
        1 gctcgctaaa acagaggggg aacaggttaa aaggggaaga ggcgaaaagg taaacaaagc
       61 gacttgcatt cttacctctt cccctcttct ccttttaacc ttttcccctc tatttgtctg
      121 tcttgtcctt cccgccgcgc cgcagacttt cggggacgcg gcgcggcttc ttgtcgggga
      181 tgaggtcgcc cgtgaacatc tcgacgacgg ccttcgccgt gccgacgacc ggcagcaggt
      241 cgaggccgac gtgcgccgcc ccgcgcagcg cgcccttctt cctgatctcg tgcccggcca
      301 gcccgaccgt catcgccgtc ccgaccaacg ggagcggctt caacagacgc ttcgccagac
      361 gcaacgcccc ggcgcgcgcc agcctccgct tcaacgccgc cctcttccgc gccctgtcat
      421 tcatcttctc ctcccgaatg ccgtaaacag taaaccgtaa accgtgacaa gtgaaagcag
      481 cgaccagtga cgagtgacaa gaagcacgct gttccgactc gtcacggttt acggttcacg
      541 gttcacggct tttcagcatc gcgccgtgcg cggcagcatg aacgcgcggc ccgcggcggc
      601 gaacgggccg cgcaccatca ggtggtcgtc gccgtcgagc aggtgcggct cggggagcgc
      661 gccttcggcg ccggcgagaa ggtctggcga gaagtagcag acgacgcgct cgacgaggcg
      721 cggcgcgcgt tcgaggatgt cgcccacggc gcaggccgcg cgcccgacca cgtcgaagag
      781 gtgcagcgtc gcgccctcga actccgccga gacgatcacg tccagctcgt ccgcgtagag
      841 cagcgggcgg aacgtttcgt tgaaggcgaa gacggcgacc tcgccctcca cgccgacgac
      901 ccgcgacact gcgggacgcc cctccagcag ccttctcaag atcgaaaggt cttcggcctc
      961 cgcgggccgc agctcacgga aacctccgcg cgcgccctcc gaaccgaccg ccagaccgaa
     1021 gcggtgctcg ccgacgtggc ggaagccgaa aggctcgtag agttcgggct gcgccgtcgt
     1081 cagcaccagc gtctcgaagc gcgcgtcgca gtagcggagc gcgtcctcca gcgtcgcgcg
     1141 gtagtgtccg cggcggcgcg cctctgcccg cgtgcagacg gcgtgaatcg cgcccgcgtt
     1201 cacccgctcg cccgcgacca ccagcggcaa cggcatcacc ccgacgtgcg acacggcccg
     1261 ccccgcttcg taacgcacga agggcttcga caccctctcc cactcgacgc cgagcgcctc
     1321 catcctcgcc agccgcgcgc cccagccgat accggggaag cctgcttcga gcagctcgaa
     1381 caactctccg ccgccgctcg acgtttctcc gcccgcgctc aacgttccag cctcgcccgc
     1441 gccttctgca aataagtccg catcatgtgc gtgatgccct tctccagctc ggcgctcaag
     1501 cgccgctcgc ccgcgaggcc ggccagcggg tgcagctcgc gcgcgttctc gcgccgcacc
     1561 tcgaccgcga gccggacgcc gacgcgcgtc atcgcgccgc gctcctcgaa agacacctcg
     1621 tagcgcaaaa agttcttccg ctcgcccgcc gccgggagcc actcgatgag gcccggcaga
     1681 tcgaccgcct gcgccaccgc gaacgcctgt cggatcgagc cgaccaccgg cacgtccgcg
     1741 cgaatcagcc agcgcgccgc cgcacccccg gcctccgccg tcacgcgctc gacgcccggc
     1801 atcagctccg cgaagttccg cagctcggcg aagaacgccc gcgccgcctc agccgccgcc
     1861 cgccactccg cctgctcgtt gaaagccgct ctgacagtaa acataaaagc cgtgaaccct
     1921 aaaccgtaaa ccgtgacagg tgaaaagccg ttaagggtga accgtaaagc ttgatgagag
     1981 gagagctgtt tggactcatc acggtttacg gtttacggtt cacggcttac ggctcttata
     2041 gaaccggttg gaacaggaac gagttgaaga agagcctcgt cgtggagcgc cagatggcgc
     2101 ggaagttggg gtcttcggcg tagaggacga cgtggccctt gccggtcggc tcgtcgatga
     2161 cgtaggcggt gccgcgcaag agtttttccg tcgtcggcca gacgaagccc gcgaggtaga
     2221 gcggctgctt cgcgtcgtcg ctgaagatga gcgcgttggt gccttccttc gaggggcgga
     2281 agaagtagcc ggaatcgacg agcacgggca acgtcgtcgc ctcgtagccg taggtcaaag
     2341 gcgtcgtcct gtccagcgtc gccttcatga tggcgccggg cactgcctcg ggcacgcgtc
     2401 cgggccgcgc cgagggcgag gcgataggag gcaggtcggg cgcggcgcct tccatcttct
     2461 cggtctgcgc ggaggagatt tgctgctgct cgggcgtcga ggcggcgggc tgctgccgtc
     2521 cgcggcgcgc ggcggactgc tcgtccgccg gtgtgggcgt gggcgaaggc gtcggcgtcg
     2581 gcgcggcgtc gccgctcggc ttctggtcgt cgtcgtcgct gccgaccaga cgcgacgaag
     2641 tcaggttcac gtccttgatc gcgccgaaga ccgccgcgcc cttgacgagc acgagcgtgc
     2701 cgccgcgctg cacccacgcg cgcaaagcgt ccacgcccgg cttgccgaac tgcgcgaagt
     2761 agccgcccgc cgagccgtcg ggcaggatga tgacgttgta gtcgtcgagc cgcgtggact
     2821 tcacggcctg aatcgtcatc ggcgtgaact ccacgcccat gcggtcgaac gtccaccaca
     2881 tcgcgccgta gccggtcgtg ctgacgggtt cgtccgcgac gacgatgatc ttcggcttgc
     2941 gcaaagagac gaccgtctcg gcgcccaccg acacgtcgcc cgtctccgtg aagcccgtgt
     3001 tgacggcggt ggcggtgacg cccgcttcct gcgcgatgcg cgcgatgcgc tcgtgcaacg
     3061 tgtcggggtt gcgcgagacg cgcacgacca ccgtcccgcg cggccactcg cgcccgcccg
     3121 ccgagagcgt ccgcgtcgcc acggcgagct tgtagtcttc tcgcaacaga cgatagacga
     3181 gcgaggccgc gccgttgctc tcatacggaa tgatgtaggc gacctgcgcg cgtcccgtca
     3241 cgccgccggg caccgaggcc gtggacacca gcgcgttacc gcccggcgcc gggaaagaag
     3301 ccccgccgac acgagtcgta aactccacca tgctcaccgg cgtgccgctg acagccgccg
     3361 cgtcctccgt ccagtaagcc tcgacgccga agacgagcgg cagagaccac gaagttatgt
     3421 cgtagaagcc gtagccttcc ttgttcgaag agcggcgctc gttgcgctcg aagcgcgcga
     3481 gctgttcgcg cgcgaaggcg gggtcgagcg gcgtgtgcat ttcgagcagc gccttggcca
     3541 gacgcttctg cggctgcgcg agatcgacga cgtaggcgcc cgccgggaag tcgcgcgagg
     3601 tggcgggcga gttctgcgac gaataatcat gcgcgcgcgc cgagcggaaa gaggcgatcg
     3661 cctgctgcac ttcgatgcct gcgcgcacga ggttctcgac cagctcggcg gcgtggccgg
     3721 ggttcctgcc gggcaggatg acgaagcggc ggattttgcc gctccttcct tcttcgaccg
     3781 ccgtgcgctt gaactcgtag tagtcgcgca cgcgcgcctc gcggttcgag gcggcggtgg
     3841 cgagcgtcgt caagctggcg acgaagtgct tggctatccc tgagcgcatg gtgacgatgg
     3901 tgtcgtcgtc gcgccgccag cgcaagccct tccagccgcc cgagtcggtc tcgtaggtca
     3961 tgccggtcgc gccgttcagc gcgggccacg agtcccagta gccggggtag aagaggtcga
     4021 agatgtcgcg gacgtagaag tcccattggc gctggtcgaa ctgcgcggcg ttggcgcgac
     4081 cgaaggcggc gagccagcgc tcggtctgtt cgggcgggag gttcgggttg acgggcaggg
     4141 ccgcgggcgg gaagaagaac tgcgagggct ggccgtggtg atcggc
//
LOCUS       DBNGOH010000793         3211 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2134931, whole genome shotgun sequence.
ACCESSION   DBNGOH010000793 DBNGOH010000000
VERSION     DBNGOH010000793.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3211)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3211)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3211
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2134931"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..864
                     /locus_tag="AC159P_15285"
     CDS             <1..864
                     /locus_tag="AC159P_15285"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HIR3696864.1"
                     /translation="YVFAYFMGNGEDGLHLAHSEDGLSWAALNGGKSLLRPSVGRDKL
                     MRDPHIVRGRDGLYHMVWTSSWTEPVIGYASSKDLIDWSEQRAIKPMEHEPQAKNVWA
                     PELFYDEDAKQYLIFWATTIPGRFPATDDTGDNKYNHRMYVTTTKDFRAFTPTRLFYD
                     DGFNVIDATIVKDGRRYVMFLKDETRQPVAKKNLRFAVAAKAAGPYGPASEPFTGRYW
                     AEGPSAIKVGKRWVVYFDKYRDKRYGAVASADLKTWEDISDRVKFPAGTRHGTVLRVP
                     RAVLGKLLELK"
     gene            1045..>3211
                     /locus_tag="AC159P_15290"
     CDS             1045..>3211
                     /locus_tag="AC159P_15290"
                     /inference="COORDINATES: protein motif:HMM:NF028442.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HIR3696865.1"
                     /translation="MKRRAEVKLSGLSVAALRLLVSLALVFPSFAFAQRRPARRGGGP
                     TSTPPVAGQPTAARDSRMTTARNVAELERHFRKPPDDSRVMMRWWWFGPTVTKPQLER
                     ELRLMKEGGIGGVEIQAVYPVILEDASNGIRNLPFLSDEFLDALRFANTKARELGLRV
                     DLTLGSGWPFGGPMVRVDQAAGALRSERVKLKTDTRRVPVPYIPAGEKFIGAFLARTR
                     GEEVVADSVRELTDIRDGAVHLPANLDGSHEVMFFIGGRAGMQVKRPAVGGEGYVLNH
                     LDRPATEHYLRTVGDRLMQAFAGAPPYAVFCDSLEVYNQDWTDDFLEEFRRRRGYDLK
                     PHLPALIADVGAKTAAIRHDWGRTQTELLNERFFVPMQQWSHRNRTRFRIQGYGIPAA
                     TIASNAHADLSEGEGPQWKVVRASRWASSANHIYGRNITSSETWTWLHSPSFRATPLD
                     VKAEADLHFLQGINQLIGHGWPYTAPGVEYPGWRFYASGVFNEKNPWWIVMPDLALYL
                     QRVSFLMRQGQPANDVALYLPNSDGWASFTAGKVHMIDVLRDHLGPEVMPAILEAGYN
                     LDFFDDDTLRQVGRVEGDALALGPNKYRAVVLPNVEHIPLDTLRKLEEFARGGGILLA
                     TRRWPEAAPGFMATEAEKREVRDISRRLFEGSAKPPAHFVEDEKRLGSRLAGALQPDV
                     SITPHFPEFGHIHRRMGDAEIYYVANTSNRRETVRAAFRV"
BASE COUNT          555 a         1083 c         1076 g          497 t
ORIGIN      
        1 tacgtcttcg cctacttcat gggcaacggc gaggacgggc tgcacctcgc gcacagcgag
       61 gacggcctct cgtgggcggc gctgaacggc ggcaagtcgc tgctgcgccc ctcggtcggc
      121 agggacaagc tgatgcgcga cccgcacatc gtccgcgggc gcgacggcct ctaccacatg
      181 gtctggacat cgtcgtggac ggagcctgtc attggctacg ccagctcgaa ggacttgatc
      241 gactggtcgg agcagcgcgc catcaagccg atggagcacg agccgcaggc gaagaacgtc
      301 tgggcgccgg agctgttcta cgacgaagac gcgaagcagt atctaatctt ctgggcgacg
      361 acgataccgg ggcgcttccc cgcgacggac gacacgggcg acaacaagta caaccaccgg
      421 atgtacgtga cgacgacgaa ggacttccgc gccttcacgc cgacgcgcct cttctacgac
      481 gacggcttca acgtcatcga cgcgaccatc gtcaaagacg ggcgccgcta cgtcatgttc
      541 ctgaaagacg agacgcgcca gcccgtggcg aagaagaatc tgcgcttcgc cgtcgccgcc
      601 aaagcggcgg ggccgtacgg ccccgcgtcc gagccgttca cgggccggta ctgggccgag
      661 gggccgtcgg ccatcaaggt cggcaagcgc tgggtcgtct acttcgacaa gtaccgcgac
      721 aagcgttacg gcgccgtcgc ctccgccgac ttgaagacgt gggaagacat ctccgaccgt
      781 gtgaagttcc ccgcgggcac ccgccacggc acggtgctgc gcgtgccgcg cgcggtgctc
      841 ggcaagctgc tcgaattgaa atagacgatt gaaagatagt tgtagggcag ggcttgtccc
      901 tacccgtcgt tggatattca ttcaacgtcg aagcaatcgg acattgacgg gccgcggact
      961 tgccctgtca ctacagttcc gacatcgttc gcagttatcc gtacaccgct cggagacgac
     1021 agagacttgg gaagggactt tcgagtgaag agacgcgccg aggtgaaact ttccggcctt
     1081 tcggtcgccg cgctgagact gctggtttcg ctcgcactcg tctttccgtc gttcgccttc
     1141 gcgcagcggc gacccgcgag gcgcggcggc ggcccgacca gcacgcctcc cgtcgcgggt
     1201 caaccgaccg ccgcgcgcga ctcccggatg acgaccgcgc gcaacgtcgc agagcttgag
     1261 cgccacttcc ggaagccgcc cgacgacagc cgcgtgatga tgcgctggtg gtggttcggg
     1321 ccgacggtga cgaagccgca gctcgaacgc gagctgcggc tgatgaagga gggcggcatc
     1381 ggcggcgtcg agattcaggc cgtctacccg gtcatcctcg aagacgcctc gaacggcatc
     1441 cggaacctgc cgttcctctc cgacgaattt ttggacgcct tgcgcttcgc caacacgaag
     1501 gcgcgcgagc tgggcctgcg cgtcgatctc acgctcggca gcggctggcc cttcggcggg
     1561 ccgatggtgc gcgtcgatca ggcggcgggc gccctgcgct ccgagcgcgt caagctcaag
     1621 accgacacgc gccgcgtgcc cgtgccgtac atccctgcgg gcgagaagtt catcggcgcc
     1681 ttcctcgcgc gcacgcgcgg cgaggaagtc gtcgccgaca gcgtccgcga gctgacggac
     1741 atccgcgacg gcgccgtcca cctgcccgcg aacctcgacg ggtcgcacga ggtgatgttc
     1801 ttcatcgggg gccgcgcggg gatgcaggtc aagcgaccgg cggtcggcgg cgagggttac
     1861 gtgctcaacc acttggacag gcccgcgacc gagcactacc tgcgcacggt cggcgaccgc
     1921 ctgatgcagg cgttcgcggg ggcgccgccg tacgcggtct tctgcgatag cctcgaagtc
     1981 tataaccagg attggacgga cgacttcctc gaagagttcc ggcggcggcg cggctacgac
     2041 ctcaagccgc acctgcccgc gctcatcgcg gacgtggggg cgaagaccgc ggccatccgc
     2101 cacgactggg gacgaacgca gaccgagctt cttaacgagc gcttcttcgt gccgatgcag
     2161 cagtggtcgc accgcaaccg cacgcgcttc cgcatacagg gctacggcat cccggcggcg
     2221 acgattgcga gcaacgcgca cgcagacctc tccgagggcg aggggccgca gtggaaggtc
     2281 gtgcgcgcgt cgcgctgggc ctcttcggcg aatcacatct acgggcgcaa catcacttcg
     2341 tcggagacgt ggacgtggct ccactcgccg agtttccgcg cgacgccgct cgacgtgaag
     2401 gccgaggccg acctccactt cctgcaaggc atcaaccagc tcatcggcca cggctggccg
     2461 tacacggcgc cgggcgtcga gtatccgggc tggcgcttct acgcgtcggg cgtcttcaac
     2521 gagaagaacc cgtggtggat cgtgatgccc gacctcgcgc tctacctcca gcgcgtgagc
     2581 tttttgatga ggcaggggca gcccgcgaac gacgtggcgc tgtatctgcc gaacagcgac
     2641 gggtgggcga gcttcacggc gggcaaggtg cacatgatcg acgtgctgcg cgaccacctc
     2701 ggcccggagg tgatgcccgc gatcctcgaa gccggttaca acctcgactt cttcgacgac
     2761 gacacgctgc ggcaggtagg gcgcgtcgaa ggcgacgcgc tcgcgctcgg gccgaacaaa
     2821 taccgcgccg tggtgctgcc gaacgtcgag cacatcccgc tcgacacctt gcgcaagctc
     2881 gaagagttcg cgcgcggcgg cggcatcctt ctcgccacgc ggcgctggcc cgaggcggcg
     2941 ccgggcttca tggcgaccga ggccgagaag cgcgaggtgc gcgacatctc gcgccgtctg
     3001 ttcgaaggct ccgcgaagcc gcccgcgcac ttcgtcgaag acgagaagcg gctcggcagc
     3061 agattggcgg gcgcgctcca gccggacgtt tcgatcacgc cgcacttccc ggagttcggg
     3121 cacatccacc ggcggatggg cgacgcggag atttattacg tcgccaacac ctcgaaccgg
     3181 cgcgagacgg tgcgcgccgc gttccgcgtc g
//
LOCUS       DBNGOH010000794         2983 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4151415, whole genome shotgun sequence.
ACCESSION   DBNGOH010000794 DBNGOH010000000
VERSION     DBNGOH010000794.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2983)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2983)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2983
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4151415"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..997
                     /locus_tag="AC159P_15295"
     CDS             <1..997
                     /locus_tag="AC159P_15295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015206222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HIR3696866.1"
                     /translation="AVVVYAGSSGLALASTPGDRKGDILGAIDRLEAGGSTNGGQGIH
                     LAYRVAEENFIRGGINRVLLATDGDFNVGVTSENELVRLIEEKRRGGVFLSVLGYGRG
                     NLKDSTMEALADKGNGNYAYIDTQGEARKVLGEQLGGTLHTSAKDVKIQVEFNPRTVA
                     GYRLVGYENRLLRDRDFNDDTKDAGEIGAGHTVTALYEVVPAGQKVENPGVDELKYGR
                     QEAPSGEMRGGELMTVKLRYKEPDGEESRLLSVAVADSNASHQNASENLKFSSAVAEF
                     GMLLRGSRYKGAATYDSALRLARAAAGADLRGHRAEFVNLVERAKTLSTQVSMRE"
     gene            complement(1266..2540)
                     /locus_tag="AC159P_15300"
     CDS             complement(1266..2540)
                     /locus_tag="AC159P_15300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005882 - intermediate filament [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HIR3696867.1"
                     /translation="MRINFRFPAIALSVLACGVFFATHHSSAQTQTQPGQVIISELRL
                     RGPAGPQDEFVEIYNNTDQDIIVQSSDGTPGWAVAISNGEVTGNIFVIPNGTRIPARG
                     HFLGAGAAYSLSGYPSGPAPQPSPTPFLGTTPDVSFDVDIPDGSGVALFNSTRGLVVG
                     AAARLDAFGFTSSPALFKEGSGFPFVPTGLGETTLFRDMRTTGGLPKDTNDNAADFLF
                     AAPTQSISTANSFLLGAPGPENLASPVLHNSDIAIGLLDPAAAPAAVPNRERRPNVEP
                     NADLGSLLIRRTFTNNTGQAVTRLRFRVFNITGPGTENTCGGPVCADVRALTSQDGEA
                     GSPNIGVVTVRGVRLEEPPLQPNGGGFNSSLSADFITLATPLQPGQSVNLQFKLGIMR
                     HGAFRFFFNIEALTAPITTNGPTALTTSAARR"
BASE COUNT          521 a          939 c         1060 g          463 t
ORIGIN      
        1 cgccgtggtc gtctacgcgg ggtcgagcgg gctggcgctc gcttcgacgc cgggcgaccg
       61 caagggcgat atcctcggcg cgatcgaccg gctcgaagcg ggcggctcga ccaacggcgg
      121 acagggcatc cacctcgcct accgcgtcgc cgaagagaac ttcatcaggg gcggcatcaa
      181 ccgcgtgctg ctcgccacgg acggcgactt caacgtcggc gtcacgagcg agaacgaact
      241 cgtccggctc atcgaggaga agcggcgcgg cggcgtcttc ctctccgtgc tcggctacgg
      301 gcgcggcaac ctgaaggact cgacgatgga ggctctggcc gacaagggca acggcaacta
      361 cgcctacatc gacacgcagg gcgaggcgcg caaggtgctc ggcgagcagt tgggcggcac
      421 gctccacacg agtgcgaagg acgtgaagat acaggtcgag ttcaacccgc gtacggtcgc
      481 cggctaccgc ctcgtcggct acgagaaccg cctgctccgc gaccgcgact tcaacgacga
      541 cacgaaggac gcgggcgaga tcggcgcggg ccacaccgtc acggcgctct acgaggtcgt
      601 gcccgccggg cagaaggtcg agaaccccgg cgtggacgag ttgaagtacg ggcggcagga
      661 agcgccgagc ggcgagatgc gcggcggcga gctgatgacc gtgaagctcc gctacaaaga
      721 gccggacggc gaagagagca ggctgttgag cgtcgccgtc gcggactcga acgcgagcca
      781 ccagaacgcc tccgagaacc tcaagttctc ctccgcggtc gccgagttcg gcatgctcct
      841 gcgcggctcg aggtacaagg gcgccgcgac ctacgactcg gcgctgcggc tcgcgcgcgc
      901 cgcggcgggc gccgacctga gaggccaccg cgcggagttc gtcaacctcg tcgagcgcgc
      961 caagacgctc tccacgcagg tcagcatgag ggagtagcca ccatcgcctc tgcgcgccgc
     1021 gcacgcgacg actgtagggg cagggcttgt ccgcggcccg tccgttcgat tgcttcaaca
     1081 tcgaacccat ctcggccgcg ggccgcggac aagccctgcc cctacagtcc ctccctacgt
     1141 tcggcactca ttccaagtct gctctaacgg tcgctcgcgt gaatagacaa aggcgcgcct
     1201 tcttcgacgg aagaaggcgc gcctaattct gtctctccgc gggaggtgag cgcgttggtc
     1261 tgtcgttaac ggcgggccgc gctcgtcgtc agcgcggtgg ggccgttggt agtgatgggc
     1321 gcggtcagcg cttcgatgtt gaagaagaag cggaacgcgc catggcgcat gatgccgagc
     1381 ttgaactgga ggttgacgct ctggccgggc tggagcggcg tggcgagcgt gatgaagtca
     1441 gccgagagcg aggagttgaa gccgccgccg ttgggctgca agggcggctc ttcgaggcgc
     1501 acgccgcgca ccgtgacgac gccgatgttg ggcgacccgg cctcgccgtc ctgcgaggtg
     1561 agcgcgcgca cgtcggcgca gacggggccg ccgcaggtgt tctccgtgcc cggccccgtg
     1621 atgttgaaga cgcggaagcg cagtcgcgtg acggcctgac ccgtgttgtt cgtgaaggtg
     1681 cggcggatga gcagcgagcc gaggtctgcg ttcggctcga cgttcgggcg ccgctcgcgg
     1741 ttgggcaccg ccgcgggcgc cgccgccggg tcgagcaggc cgatggcgat gtcgctgttg
     1801 tggagcaccg gactcgcgag gttttcgggg ccgggcgccc cgagcaggaa gctgttggcg
     1861 gtcgagatgc tctgtgtggg cgcggcgaac aggaagtcgg cggcgttgtc gttcgtgtcc
     1921 ttcggcagcc cgcccgtcgt ccgcatgtcg cggaagagcg tcgtctcgcc gaggccggtc
     1981 gggacgaagg ggaagccgct gccctccttg aagagcgcgg gcgaagaggt gaagccgaag
     2041 gcgtcgagcc gggccgcggc gcccacgacc agcccgcgcg tcgagttgaa gagcgcgacc
     2101 ccgctgccgt cggggatgtc aacgtcgaac gacacgtcgg gcgtagtgcc gaggaacggc
     2161 gtgggcgaag gctgcggcgc cgggccggac gggtagccgc tcaggctgta ggcggcgccg
     2221 gccccgagga agtggccgcg cgccggtatc cgcgtcccgt tcgggatgac gaagatgttg
     2281 ccggtcacct ctccgttcga gatcgccacg gcccatcccg gcgtgccgtc gctggactgg
     2341 acgatgatgt cctgatcggt gttgttgtaa atctcgacga actcgtcctg cggccccgcg
     2401 gggccgcgta ggcggagttc gctgatgatg acctggccgg gttgtgtctg tgtctgggcg
     2461 gaagagtggt gagtcgcgaa gaacacgccg caggcgagca cagacagcgc tatcgcggga
     2521 aagcgaaagt tgatacgcat ggatcctccc gtaaatggtt aacggttgaa ctcgtccgtg
     2581 tggacgggga cttgaatagg gagacagtcc tcgcgggagt gagagaaata cctcgcccgc
     2641 gaaattaatc gcgcgatgat actcagggag acttgtccta atgtggcaga aaaacgcccc
     2701 tatcaataga acattccggg ccgttcttcc gtccacaatc gtacaaaaat cgcccgcgag
     2761 acggcctccg cgttaagaat cttcttcaaa ctgttaacgc gtagtccggc gacgaattcc
     2821 cacacggcga ttatggaata tacgttccgg aattcggatt gagtacgtcc acatggacgg
     2881 gcgaagagag cacgactctc ttcgccgacc tcgcgtgacg cgggacggcg gccctctcgt
     2941 cgaccacgaa tccgcgcccg gcacgccgca cgccgccgcc gcc
//
LOCUS       DBNGOH010000795         4327 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_852121, whole genome shotgun sequence.
ACCESSION   DBNGOH010000795 DBNGOH010000000
VERSION     DBNGOH010000795.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4327)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4327)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4327
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_852121"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..568
                     /locus_tag="AC159P_15305"
     CDS             <1..568
                     /locus_tag="AC159P_15305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696868.1"
                     /translation="FRARYTQRITPWALTEYDEDGEAIVHGYCDGTETEVLFELQNLT
                     EVGPEGFRAQELYVGLCGLAYRARVSQSRTERAAWQPLEECAPSRAAHENDWSLTGRV
                     VSFKPLRNPLSGGELYWVYLDLERLKLEVLVNRRTLRGELQLGAMLSAEVWLQGHVLD
                     RRALLSRYEGADTSYTKADLWSHLRRRN"
     gene            623..2014
                     /locus_tag="AC159P_15310"
     CDS             623..2014
                     /locus_tag="AC159P_15310"
                     /inference="COORDINATES: protein motif:HMM:NF021982.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3696869.1"
                     /translation="MRNPLLLLLPLFLILPTAHASAQKITRETFLVRGKTRTYYLYVP
                     AKKQAGATMPLVLTLHGSGRDGRLLVEHWKDLAEREGFILAGPDSKTSAHWSMKDDGA
                     ELFVALAEELKSKQPVNPRRVYLFGHSAGAGFALLLALFESRYFTAIAVHAGVLLPST
                     YPLMERAPRKMPLAIYVGTQDPLFPVTAVRATRDALKQRGYAPELTEIKFHDHNYYDR
                     AKEINRSAWEFLSRHELAEAPQLDKGSAAGQGGATVDGNLPTPEQASAPLTQANGAAA
                     EVFERAARLYEAGDAGGALAAYTKGIELDPNVAGAYNNRALAHSLLGNHETAIADLTR
                     SIALDPTLAAAYVNRGAAYRALKRYEEAVADFTKATRLARSPEAFFNRGVTYEYMGDP
                     EAALKDYGNAISLDPRLAPAYVNRGIILLQQGHESEAARDFETGFELDPSLRAEVGDY
                     IEQLRASRRKKRP"
     gene            2087..2683
                     /locus_tag="AC159P_15315"
     CDS             2087..2683
                     /locus_tag="AC159P_15315"
                     /inference="COORDINATES: protein motif:HMM:NF013618.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HIR3696870.1"
                     /translation="MPLLKRIALVLLVLLTLAAFGLYFFNRYWVQRFDPLIARQAAVY
                     RLDPDLVWSIIHEETYFRPWMVGEDEEIGLMQVTPAVGREWAAETGMRELSEQMRRDP
                     ASLLRDPERNIQIGCWYLEKIWKAYRDQPGAEARVLAAYNAGPSRAADWNRLAPANQS
                     FTAEQFIERIDIPSTRAYVSNILTRYRARKEKAAGSRR"
     gene            2867..4219
                     /locus_tag="AC159P_15320"
     CDS             2867..4219
                     /locus_tag="AC159P_15320"
                     /inference="COORDINATES: protein motif:HMM:NF017760.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3696871.1"
                     /translation="MTYRQLLAGNADFRRLWTGQVVSEIGDWLNNIAVLALAIQLAGA
                     GRVGLAIAVYAIARHLPLFLFGPVAGVVVDRVDRRRVMIAADLLRALLALGFLFADRT
                     HSLAVIYAVGASLFSVSSFFNAAKRATIPNLVRDTDELLAANSLSASTTAATIAVGSA
                     LGGVAATFLGRDAVFVLNALTFLASAEMIRRVSSKQRAVSRERTLEDERAKHHAPTAH
                     RSLLTAYFMDFREGLRYVRREPTLFAVFVVAAGWGLGNGAARALYSIFGARLGEEYVA
                     GWGGAARELVERPTDFGISVLFVAMGLGGVMGAPLARRFNSAAGDSLGARMGRSLALD
                     GLGLFLFSLMPSLWAAALVLVAREMNFAIWWTAQQTLMMKRTQDAFAGRVFASYETLT
                     TLMMVGAMLASGAAADRVGIRPVASAGGLVITLSGLLWFALRSRRADDSAERATTENA
                     "
BASE COUNT          695 a         1547 c         1393 g          692 t
ORIGIN      
        1 ttttcgcgcg cgctacacgc agcgcatcac gccgtgggcc ttgaccgagt acgacgagga
       61 cggcgaggcc atcgtccacg gctactgcga cggcaccgag acggaagtcc tcttcgagtt
      121 gcagaacctg acggaggtcg gccccgaggg cttccgcgcg caggagctgt acgtcggcct
      181 gtgcgggctg gcctaccgcg cgcgcgtctc gcagtcgcgt accgagcgcg ccgcgtggca
      241 gccgctcgaa gagtgtgcgc cgtcgcgcgc cgcgcacgag aacgactgga gcctgacggg
      301 ccgcgtcgtc tccttcaagc ctctgcgcaa cccgctctcg ggcggcgaac tgtactgggt
      361 ctacctcgac ctcgaacggc tcaagctcga agtgctggtc aaccgccgca cgctgcgcgg
      421 cgagcttcag ctcggcgcga tgctctcggc ggaggtctgg ctgcaaggcc acgtgctcga
      481 ccgccgcgcg ctcctctcgc gctacgaggg cgccgacacc tcctacacga aggccgacct
      541 gtggtcgcac ctgcggcgcc ggaactagat cgtgcccgtc atcgtcaacc gccagtcggt
      601 tgtgaacccg cccgggagct tcatgagaaa cccgctgtta ctgctcctgc cgctcttcct
      661 tatcctcccc acggcgcacg cctcggcgca gaagatcacg agagagacgt tcctcgtcag
      721 agggaagacg cgcacttact atctctacgt ccccgcgaag aagcaggcgg gcgcgaccat
      781 gccgctcgtc ctgacgctgc acggctcggg gcgcgacggc aggttgctcg tcgagcactg
      841 gaaagacctc gccgagcgcg agggcttcat cctcgccggc ccggactcga agacttcggc
      901 gcactggagc atgaaggacg acggcgccga gctgttcgtc gcgctcgccg aggagctgaa
      961 gtcgaagcag ccggtcaacc cgcggcgcgt ctacctcttc ggccactcgg cgggcgcggg
     1021 cttcgcgctc ctgctcgcgc tcttcgagtc gcgctacttc acggcgattg ccgtccacgc
     1081 gggcgtgctc ctgccgagca catacccgct gatggaacgc gccccgcgca agatgccgtt
     1141 ggcgatttac gtcgggacgc aagaccctct gttccccgtg acggcggtgc gcgcgacgcg
     1201 cgacgcgctg aagcagcgcg gctacgcgcc cgagctgacc gagatcaagt tccacgacca
     1261 caactactac gaccgcgcga aggagattaa ccggagcgcg tgggagttcc tgagcaggca
     1321 cgaactcgcg gaagcgccgc aactcgataa aggctcggcg gcggggcagg gcggggcgac
     1381 ggttgacgga aacctcccga cgcccgagca ggcgagcgcg ccgctcacgc aagcgaacgg
     1441 cgccgccgcc gaagtcttcg agcgcgcggc caggctctac gaggcgggcg acgcgggcgg
     1501 cgccctcgcc gcctacacga agggcatcga gttagacccg aatgtcgcgg gcgcttacaa
     1561 caaccgcgcc ctcgcgcact cgctgctggg caaccacgag acggccatcg cagacctcac
     1621 gcgcagcatc gcgctcgacc cgacgctcgc cgccgcctac gtcaaccgcg gcgcggccta
     1681 ccgcgcgctc aagcgctacg aagaggccgt cgccgacttc accaaggcca cgcggctcgc
     1741 ccgctcgccc gaagccttct tcaaccgcgg cgtcacgtac gagtacatgg gcgaccccga
     1801 ggcggccctc aaagactacg ggaacgccat ctcgctcgac ccgcggctcg ccccggccta
     1861 cgtcaaccgc ggcatcatcc tgctgcaaca gggtcacgag tcggaggccg ccagagactt
     1921 cgagaccggt ttcgaactcg acccctcgct gcgcgcggag gtcggcgact atatcgagca
     1981 gttacgcgcg tcgcgcagga agaaacggcc ctgacccccg cgcctcccgc gtcgcccgcc
     2041 cgacgggctt gacgcgcgcg cacggtgctt gtcacgatta accttgatgc cgctgctgaa
     2101 aagaatcgcg ctcgtcctgc tcgtcctcct cacgctcgcc gccttcggcc tctacttctt
     2161 caaccgttac tgggtgcagc gcttcgaccc gctcatcgcg cggcaggccg ccgtctaccg
     2221 cctcgacccc gacctcgtgt ggagcatcat tcatgaagag acctacttcc gtccgtggat
     2281 ggtgggcgaa gacgaagaga tcgggctgat gcaggtgacg cccgcggtcg ggcgcgagtg
     2341 ggccgccgag accgggatgc gcgaactctc ggagcagatg cgccgcgacc ccgcgagcct
     2401 gctccgcgac cccgagcgca acatccagat cggctgctgg tatctggaaa aaatctggaa
     2461 ggcctatcgc gaccagcccg gcgcagaggc gcgcgtgctc gccgcctaca acgccggccc
     2521 ctcgcgcgcc gccgactgga accgcctcgc ccccgccaac cagtccttca ccgccgaaca
     2581 gttcatcgaa cgcatcgaca tcccctccac ccgcgcctac gtctcgaaca tcctcacgcg
     2641 ctaccgggcg aggaaggaga aggcagccgg cagtaggcgg taggcagtag gcagtcaaga
     2701 caagatagat ttcaaattcc aaatctccgg tttggtcatc agcgcttgcc tgcgtttcgg
     2761 ttgaagggtt aaagatttca agcatgagat ttgaaattca gcctccgcga ccgctcggac
     2821 tgcctactgc ctactgccta ctgcctactg ttgcgcggag cggcctgtga cctaccggca
     2881 actgctcgcg ggcaacgccg acttccgacg gctgtggacg gggcaggtcg tctcggagat
     2941 cggcgactgg ctgaacaaca tcgccgtgct ggcgctcgcc atccagctcg cgggcgcggg
     3001 gagggtcggg ctggcgattg ccgtctacgc catcgcgcga cacctgccgc tcttcctgtt
     3061 cggccccgtc gcgggcgtcg tggtcgatag ggttgaccgc cgccgcgtga tgatcgcggc
     3121 agacctgctg cgcgcgctgc tcgcgctcgg cttcctcttc gcggacagga cgcactcgct
     3181 cgccgtcatc tacgccgtcg gcgcgtcgct cttctcggtc tcctctttct tcaacgccgc
     3241 gaagcgcgcg acgattccga acctcgtgcg cgacacggac gagctgctgg ccgccaactc
     3301 gctgtcggcc tcgaccacgg cggcgaccat cgccgtcggc tccgcactcg gcggcgtcgc
     3361 cgcgaccttc ctcgggcgcg acgccgtctt cgtcctcaac gcgctcacct ttctcgcctc
     3421 cgccgagatg atcagaaggg tgagcagtaa gcagagagca gtgagcagag aaagaactct
     3481 cgaagacgaa cgagcgaagc atcacgcgcc gactgctcac cgctcactgc ttactgctta
     3541 ctttatggat ttccgcgagg ggctgcgcta cgtgcggcgc gagccgacgc tcttcgcggt
     3601 cttcgtcgtg gccgccgggt ggggcctcgg caacggcgcg gcgcgcgctc tctacagcat
     3661 cttcggcgcg cggctcggcg aggagtacgt ggccggttgg ggcggcgcgg cgcgcgaact
     3721 cgtcgagcgc ccgacggact tcggcatctc cgtgctcttc gtcgcgatgg ggttgggggg
     3781 cgtgatgggc gctccgctcg cgcgcaggtt caacagcgcg gcgggcgact cgctgggcgc
     3841 gcgcatgggc cgctcgctcg cgctcgacgg cctcggcctc ttcctcttca gcctgatgcc
     3901 gagcctctgg gcggcggcgc tcgtcctcgt cgcgcgcgag atgaacttcg ccatctggtg
     3961 gacggcgcag cagactttga tgatgaagcg cacgcaggac gccttcgccg gtcgcgtctt
     4021 cgcctcgtac gagacgctga cgaccctgat gatggtcggc gcgatgctcg cgtccggggc
     4081 ggcggcagac cgcgtcggca tccgcccggt tgcctccgcg ggcggccttg tgattacctt
     4141 gtccggcctg ctctggttcg ccctgcgctc gcgccgcgcc gacgattcag ccgaacgggc
     4201 gacgaccgag aatgcctgac gaagccacac gacgaccaag catgacgacc agaatctacg
     4261 ccgcgcgctg ggtgctgccg atctcttccg agacggtcga gaacggcgcg gtcgccgtca
     4321 gcggcac
//
LOCUS       DBNGOH010000796         2404 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3970210, whole genome shotgun sequence.
ACCESSION   DBNGOH010000796 DBNGOH010000000
VERSION     DBNGOH010000796.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2404)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2404)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2404
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3970210"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1375
                     /locus_tag="AC159P_15325"
     CDS             <1..1375
                     /locus_tag="AC159P_15325"
                     /inference="COORDINATES: protein motif:HMM:NF015722.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HIR3696872.1"
                     /translation="QIVSALSGYRRRLGPESNLLVYYAGHGFNDREADKAYWLPVDAE
                     RDDTSNWIIADDITTALKVIPSRHVLVVSDSCYSGTLTRGLGEALPPANEREQFLQRM
                     LAGRSRTLMASGGDEPVADGGGGGKHSVFAGALLRGLREMDKGKFTAAELFRHYVEER
                     VAGRAEQTPEYNPLRNSGHQSGDFVFVRVKTGGKTVEVTVKAPAAPAADPEAGRQPLA
                     DGAAGGPPPPNPKSGTVVRNRSGMELVYVPPGEFMMGATNGGAGERPVHRVTIREGFY
                     MGKYEVTQAQWQAVTGNTFVGCDTCPVEQVTWDDAQSFIRKLNGQDDGFHYRLPSEAE
                     WEYACRAGSTQDHAGYLDALAWYDANSGKKTQPVGQKQPNAFGLYDMHGNVWEWVQDY
                     WHANYDGAPADGSAWLSGGDTTKRVMRGGSWFNQPSALRSAQRSGQRPGLRINSVGFR
                     VAAVARQ"
     gene            1598..>2404
                     /locus_tag="AC159P_15330"
     CDS             1598..>2404
                     /locus_tag="AC159P_15330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TolB-like translocation protein"
                     /protein_id="HIR3696873.1"
                     /translation="MNRRTERDESVSARRVAASPRAEGFARKAALALLLLSACLLAGA
                     CKEAGEALSVAPRSLRDVPAERLAFRFEPDAKEEALPERLKTTEAEEPLVSVKADFEG
                     RRGNTEALIRTVLDPTGQRALALYGTSETETDFRIDLYSAEGQFIRNVLPKDLTGVFP
                     AEVAWSPDGQSIAFVGIRNPALAAPTPPPDTAPPLTDPALPNVDPNATPTPVAPLIAA
                     VPVFKTEQVYVGDRDGFKLRPLTSREGLIYFQLAWSPDGQALAALACKEDE"
BASE COUNT          382 a          808 c          868 g          346 t
ORIGIN      
        1 gcagatcgtc tcggcgctgt cgggctaccg gcgccggctg gggcccgagt cgaatctact
       61 cgtctactac gccggccacg gcttcaacga cagggaggcg gacaaggcct actggctgcc
      121 cgttgacgcc gagcgcgacg acacgtcgaa ctggatcatc gccgacgaca tcacgaccgc
      181 gctcaaagtc atcccgtcgc gccacgtcct cgtcgtctcc gatagctgct attcggggac
      241 gctgacgcgg gggctgggcg aggcgctgcc gccggccaac gagcgcgagc agtttttgca
      301 gcggatgctg gcggggcgct cgcggacgct gatggcttcg ggcggcgacg aacccgtggc
      361 cgacggcggc gggggcggta agcattcggt cttcgcgggc gcgttgctgc gcggcctgcg
      421 cgagatggac aagggaaagt tcaccgccgc cgagctgttc cgccactacg tcgaggagag
      481 ggtcgcgggc cgcgccgagc agacgcccga atacaaccct ttgagaaact ccggccacca
      541 gagcggcgac ttcgtcttcg tccgcgtcaa gacgggcggg aagacggtcg aggtgacggt
      601 caaggctccg gcggcgccgg cggctgaccc ggaggctggc cgccagccgc tggcggatgg
      661 tgcggcgggc ggcccgccgc cgccgaaccc gaagtccggg acggtggtgc gcaaccgttc
      721 ggggatggag ttggtctacg tcccgccggg cgagtttatg atgggggcga cgaacggggg
      781 cgcgggcgag cggcccgtgc accgcgtgac gatacgagaa ggcttttaca tgggcaagta
      841 tgaggtgacg caggcccaat ggcaggcggt gaccggaaac acctttgtcg gctgcgacac
      901 ctgccccgtg gagcaggtga cgtgggatga cgcgcaatct ttcatccgga agctgaacgg
      961 gcaggacgac gggtttcatt accgcctgcc gtcggaggcg gagtgggaat acgcgtgccg
     1021 cgctggctcg acccaggatc acgcgggtta tctcgacgcg ctggcgtggt acgacgccaa
     1081 ctccggcaag aaaacgcagc cggtcgggca gaagcagccg aacgcgttcg ggctgtacga
     1141 catgcacggc aacgtctggg agtgggtgca ggactactgg cacgcgaact acgacggcgc
     1201 gccggcagac ggctcggcct ggctgagtgg cggcgacaca accaaacggg tcatgcgcgg
     1261 cggctcgtgg ttcaaccaac cttccgccct ccgctccgcc cagcgcagcg ggcaaaggcc
     1321 gggcctgcgc atcaacagcg tcggcttccg ggtggcggcg gtcgcgcggc agtgattttc
     1381 tgctgacgag ctgtcctgag gctggtcaac tcgcccgcgc gcgactcgcc ggaggtgctg
     1441 cggcccgcgg cgtgacgcgc atcggggcgt cgtggcggcg gctgaaaaac cggcaaagag
     1501 accgcccgca aggtctgcgt caagacctcg cgggcggtct cctctgtggg gcgcgttgac
     1561 accgcgtcgc cctgctctct atgattttcg cttgaacatg aaccgtcgaa ccgaacggga
     1621 cgagtccgtg agcgcacgcc gcgtcgccgc atcgccgcga gcggagggct tcgcgcggaa
     1681 agcggcgttg gcgctgctgc tgttgtcggc gtgcctgctc gccggcgcgt gcaaggaggc
     1741 gggcgaggct ttgagcgtcg cgccgcgcag cctgcgcgac gtgcccgcgg agcgtctggc
     1801 gttccgcttc gagccggacg cgaaggagga ggcgctgccc gaacggctga agacgaccga
     1861 agccgaggag ccgctggtct ccgtcaaggc cgacttcgag gggcgtcgcg gcaacacgga
     1921 ggcgctgatc cgcaccgtgc tcgacccgac ggggcagcgc gcgctcgccc tctacggcac
     1981 gagcgagacg gaaacggact tccgcatcga cctctactcg gcggaggggc agttcatccg
     2041 caacgtcctg ccgaaagatt tgacgggcgt cttcccggcg gaggtcgcgt ggtcgcccga
     2101 cgggcagtcc atcgccttcg tcggcatccg caaccccgcg ctcgccgcgc ccacgccgcc
     2161 gcccgacacc gcgcccccgc tcacggaccc cgcgctgccc aacgtcgatc ccaacgccac
     2221 gccgacgccc gtcgcgccgc tcatcgccgc cgtgcccgtc ttcaagaccg agcaggtcta
     2281 cgtcggcgac cgcgacggct tcaagctgag gccgctcact tcgcgcgagg ggctgatcta
     2341 cttccagctc gcgtggtcgc ccgacggcca ggcgctcgcc gcgctcgcct gcaaggagga
     2401 cgag
//
LOCUS       DBNGOH010000797         2216 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1953227, whole genome shotgun sequence.
ACCESSION   DBNGOH010000797 DBNGOH010000000
VERSION     DBNGOH010000797.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2216)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1953227"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..652)
                     /locus_tag="AC159P_15335"
     CDS             complement(<1..652)
                     /locus_tag="AC159P_15335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017954005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR3696874.1"
                     /translation="MQTVPEAEAAAARAQASGGAEELVRVRALYKHFPVKASDDVVRA
                     VDGVSFDIFRGETLGLVGESGCGKSTVGRCLLRLIEPTGGQISFEGRDVRSLAGGDLR
                     RLRREMQIIFQDPYASLNPRMKVRDIIAEPLVIHAIGERAERRERVADLLRKVGLDPD
                     YMNRYPHEFSGGQRQRLGIARALALNPKLIVADEPVSALDVSVQAQVINLLEDLQGE"
     gene            complement(827..1864)
                     /locus_tag="AC159P_15340"
     CDS             complement(827..1864)
                     /locus_tag="AC159P_15340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005487365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3696875.1"
                     /translation="MPHLLEVKNLQTHFPTRAGLVRAVDGVSFHVDEGELLGLVGESG
                     CGKSITALSVMRLIAPPGKIAGGELSFAGENLLAASDERMREIRGDDIAMIFQDPMTS
                     LNPVYTVGEQIAEALRLHRRMNRRQAREAAVKAMHEVAIPDPARRADDYPHQLSGGMR
                     QRVMIAMALACDPRLLIADEPTTALDVTIQAQILELIDGLRRTRNLAVLLITHDLGVV
                     AEVADRVAVMYTGRIVEESPVGELFARPKHPYTEGLLRSVPKLTEAEVKKAVRLSTIE
                     GTVPKPTNLPAGCHFAPRCPHRMPRCTEGEIPLYQLEHGVSARCVLYDLAAAVAADHR
                     IEERQGGWAVE"
     gene            complement(1925..>2216)
                     /locus_tag="AC159P_15345"
     CDS             complement(1925..>2216)
                     /locus_tag="AC159P_15345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="YqhG/Tai3 family protein"
                     /protein_id="HIR3696876.1"
                     /translation="NWTPLENRREALKYLTPAWRVRAPRVSGDEGVNVFVCAQDWLAE
                     WRKTMTFAPAVVRGTRATARVTWSTGPDTQRIDVTLKKVGGAWKIDRTSCAL"
BASE COUNT          341 a          819 c          705 g          351 t
ORIGIN      
        1 actccccctg caagtcttcc aagaggttga tgacctgcgc ctgcaccgac acgtcgagcg
       61 cagagaccgg ctcgtccgcg acgatgagct tcgggttcag cgccagcgcg cgcgcgatgc
      121 cgaggcgctg ccgctgcccg cccgagaact cgtgcgggta gcggttcatg tagtcggggt
      181 cgagtccgac cttccgcagg aggtcggcca cgcgctcgcg ccgctcggcg cgctccccga
      241 tggcgtggat gacgagcggc tccgcgatga tgtcgcgcac cttcatgcgc gggttcaagg
      301 aggcgtaagg gtcttggaaa atgatctgca tctcgcgccg cagccgccgg aggtcgccgc
      361 ccgcgagcga gcgcacgtcg cgcccctcga acgagatctg ccccccggtc ggctcgatca
      421 ggcgcagcag gcaacgcccc accgtcgatt tgccgcaccc cgattcgccc accagcccca
      481 gcgtctcgcc gcggaagatg tcgaacgaca cgccgtccac cgcccgcacc acgtcgtcgc
      541 tcgccttcac cgggaagtgt ttatagagcg cgcgcacgcg caccaactct tccgcgccgc
      601 ccgacgcttg cgcgcgcgcc gccgcggcct ctgcttcggg aaccgtctgc attctctcac
      661 tcgtgctcat gactcaggac gcgcgtataa aagcgcagcg cacgctaaac tgtaggggca
      721 gggcttgtcc ctgcccgcgg cgctcgatgc gacgtaacgt aaacggcaat gaagaccgcc
      781 gggcagggac aagccctgcc cctacaaatg tactgctcgc cagtgctcat tcgaccgccc
      841 accctccctg ccgctcctcg attcgatggt cggcggccac cgccgcggcg aggtcgtaga
      901 gcacgcagcg cgcgctgacg ccgtgctcaa gttggtagag cggaatctcg ccctcggtgc
      961 agcgcggcat gcggtgcggg cagcggggcg cgaagtggca ccccgcgggc aggttcgtcg
     1021 gcttcggcac cgtgccctcg atggtcgaga gccgcacggc cttcttcacc tcggcctcgg
     1081 tcagcttcgg caccgaacgc agcagccctt ccgtgtacgg gtgcttgggc cgcgcgaaca
     1141 gctccccgac cggcgactcc tcgacgatgc gccccgtgta catcacggcc acgcgatccg
     1201 cgacctcggc cacgacgccg aggtcgtgcg tgatgagcag cacggcgagg ttgcgcgtcc
     1261 tccgcagccc gtcgatcagc tcaagtatct gcgcctgaat cgtcacgtcg agcgcggtcg
     1321 tcggctcgtc ggcgatcagg agtcgcgggt cgcacgcgag cgccatcgcg atcatcacgc
     1381 gctggcgcat cccgcccgac aactggtgcg ggtaatcgtc ggcgcgccgc gccgggtcgg
     1441 ggatggcgac ctcgtgcatc gccttcacgg cggcctcgcg cgcctgcctg cggttcatcc
     1501 ggcggtgcag cctgagcgcc tcggcgatct gctcgccgac ggtgtagaca gggttcagcg
     1561 aagtcatcgg gtcttgaaag atcatcgcga tgtcgtcgcc gcgaatctcg cgcatccgct
     1621 cgtcggacgc ggcgagcaaa ttctcgcccg cgaagctcaa ctcgccgccc gcgatcttcc
     1681 ccggcggcgc aatcagccgc atcacagaca gcgccgtgat cgacttgccg cacccggact
     1741 cgcccaccag ccccagcagc tcgccctcgt ccacatgaaa gctcacgccg tccaccgcgc
     1801 gcaccaaccc cgcgcgcgtc gggaaatgcg tctgtaggtt tttgacttcg agcaggtgcg
     1861 gcatcgtgct ccgagattaa ccgagacgtt ctcctgatgc caagacgcgg ggcgcgcggc
     1921 ggcgtcacag ggcgcacgac gtacggtcga tcttccacgc gccgccgacc ttcttgagcg
     1981 tgacgtcgat ccgctgcgtg tccgggccgg ttgaccaggt gacgcgcgcc gtggcccgcg
     2041 tgccgcgcac gaccgcgggc gcgaacgtca tggtcttgcg ccactcggcc agccagtcct
     2101 gcgcgcagac gaagacgttc acgccctcgt cgccgctcac gcgcggcgcc ctcacgcgcc
     2161 acgcgggcgt cagatacttc aaagcctcgc gccggttctc caacggcgtc caattg
//
LOCUS       DBNGOH010000798         2357 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_669243, whole genome shotgun sequence.
ACCESSION   DBNGOH010000798 DBNGOH010000000
VERSION     DBNGOH010000798.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2357)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2357)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2357
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_669243"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            822..>2357
                     /locus_tag="AC159P_15350"
     CDS             822..>2357
                     /locus_tag="AC159P_15350"
                     /inference="COORDINATES: protein motif:HMM:NF025885.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 interaction domain-containing protein"
                     /protein_id="HIR3696877.1"
                     /translation="MTGLPLLFPASAPEDGGETSFQLPLASMVRALCRAANSGSDVLY
                     DVMIKLALMVDATAPTYGLATFSQKNGERPNLKWVEGLDQEEIAVAEDRVCDALNKTD
                     GALEVKAGDLNICMILSFATWGREGAAIYGRFVRPLTERQALELRIVSDVAQLAHAHA
                     LLREELHRPAQAPSHARIAAASLPGMVFVSRAMSALAHSIERIKDSESTVLIEGESGT
                     GKELIARAIHRLSRRAEAPFIPFNCTAAPADLIESLLFGHRKGSFTGAHADHEGLVRA
                     AEGGTLFLDEIGDLPLALQPKLLRFLQEGEVHTLGERTPRRVNVRVLAATHKDLLKAV
                     DAGTFREDLYYRVASLSLNVQPLRERPEDIGALISHYLTHYARRNDRAIAGITAEAIN
                     VLHNYSWPGNVRELAAEIERLVLYTDEGSYIGVEHINPRIRPARPQDGEAGAATTAPA
                     DLENMLEDYERRVITETLRRNDYNVARAATALGLGSRQTLYKKLKRLAINVGDFLHDD
                     QQPG"
BASE COUNT          467 a          693 c          779 g          418 t
ORIGIN      
        1 gaagggaacg aaggatgcga agccgcgagg gcgcgcgccg tggggcgctg cgaagagagt
       61 cgggagtcag tccaggtaaa attcgttgcc atattcggga gcacctccaa gccttgttcg
      121 ggttcaaaat tgacgacaga aaggtcgtca acgaatctcg tctgaaagct ttcggagggg
      181 tgaaagctca agatactgtg gcctcgttcg gttgaggccc aagatgcagt gctggaaaaa
      241 tctttattcc tttatcgctc aagaagttag cgataaagtg ggcggaatgt agcatgcgta
      301 ctgtacgctg taaagagttt tcttatcaac gctttatgcg gctaaattca acatttactt
      361 gaagtaaggg catttaacct attctcctgg cgtacaggtg gcgaacggaa atccgccgag
      421 tgggcaagcc gtccggatgg ctttacgagg cagcaagata gggtcgaaat tcggccatca
      481 caggtgtgga aaaattcggt ttatccgggc gggcggacac cgggaaaggc gggttcgtct
      541 ctcggtgagg gcgtggccga aggcgttttg cgccctcgcg cgaagagggc gacgcaatta
      601 aacaagtaat tggttgacac ggttaaacgt gggagactaa actgttgaag gttccgaatc
      661 gaggctctct ccttcggact tccggcccgg acggcgagac atagacagac ccatcgttcg
      721 tcgtttaact gccccaattt catcctcaaa gtctctttgc ggcggacgtg tgtccacgcc
      781 gctgttaata atcccaaccg aacagggtga ttaggtcgtt tatgactggc ttaccgctcc
      841 tctttcccgc cagcgctccg gaagacggcg gggagacttc cttccagcta cctctggcct
      901 cgatggtgcg ggcgctgtgc cgcgcggcga attccggctc cgacgttctc tacgacgtga
      961 tgatcaagct ggcgctgatg gtcgatgcga cggcgccgac gtacggcctg gcgaccttct
     1021 cgcagaagaa cggcgagcgc ccgaatctga agtgggtcga ggggctggat caggaagaga
     1081 tcgccgtcgc ggaggacagg gtgtgcgacg ccttgaataa aaccgacggc gcgttggagg
     1141 tcaaggccgg cgacctgaac atctgcatga tcctgtcctt cgccacgtgg gggcgtgagg
     1201 gcgcggcgat ctacggtcgc ttcgtgcgcc cgctgaccga gcggcaggcg ctcgaactga
     1261 ggatcgtctc ggacgtggcg cagctcgcgc acgcgcacgc gctgctgcgc gaggagttgc
     1321 acagaccggc gcaggcgcct agccacgcgc gcatcgcggc ggcgtctttg cccggcatgg
     1381 tcttcgtcag ccgcgcgatg tccgcgctgg cgcactcgat cgagcgcatc aaggactcgg
     1441 aatcgacggt gctcatcgag ggcgagtcgg ggacgggcaa ggagctgatc gcgcgcgcga
     1501 ttcaccgtct gagccggcgc gcagaggcgc cgttcatccc cttcaactgc acggcggctc
     1561 ccgccgacct gatcgagagt ctactgttcg gccaccgcaa aggctcgttc accggcgcgc
     1621 acgctgacca cgaaggtctg gtgcgcgcgg cggaaggcgg aacgctcttc ctggacgaga
     1681 tcggcgatct gccgctcgcg ctacagccga agctgctgcg cttcctgcaa gagggcgagg
     1741 tgcacacgct cggcgagcgg acgccgcggc gcgtgaacgt gcgcgtgctg gcggcgacgc
     1801 acaaagacct gttgaaggcg gtggacgcgg ggacgttccg cgaagacctc tactaccggg
     1861 tcgcctcgct ctccctcaac gtgcagccgc tgcgagagag gccggaggac atcggcgcgc
     1921 tcatctcgca ttacctgacg cactacgcgc ggcggaacga ccgggccatc gcgggcatca
     1981 cggcggaagc gatcaacgtg ctccataatt attcgtggcc gggcaacgtg cgcgagctgg
     2041 cggcggagat cgagcggctg gtgctctaca cggacgaagg ctcctacatc ggcgtcgagc
     2101 acatcaaccc gcgcatcagg ccggcgcgtc cgcaggacgg cgaagcgggc gcggcgacga
     2161 cggccccggc tgacctcgaa aacatgctcg aagattacga gcggcgcgtc atcacggaga
     2221 cgctgcgccg caacgactac aacgtcgcgc gcgcggcgac ggcgctcgga ctcggctcgc
     2281 gccagacgct ctacaagaag ctcaagcggc tggcgatcaa cgtcggcgac ttcctacatg
     2341 acgaccagca gcccggc
//
LOCUS       DBNGOH010000799         3336 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1953536, whole genome shotgun sequence.
ACCESSION   DBNGOH010000799 DBNGOH010000000
VERSION     DBNGOH010000799.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3336)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3336)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3336
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1953536"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(345..659)
                     /gene="rhaM"
                     /locus_tag="AC159P_15355"
     CDS             complement(345..659)
                     /gene="rhaM"
                     /locus_tag="AC159P_15355"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011092059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019318 - hexose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HIR3696878.1"
                     /translation="MIRKAFLMTLRPGVQEEYERRHNPIWPELRDVLKSHGVSNYSIF
                     LDRRRDELFAYAEVESEELWQQIAATDACRRWWSHMKELMLTNPDDSPVALPLDEVFH
                     ID"
     gene            complement(727..1959)
                     /locus_tag="AC159P_15360"
     CDS             complement(727..1959)
                     /locus_tag="AC159P_15360"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HIR3696879.1"
                     /translation="MKHVSPLSLAFVITCVVGVFFFAGADAAAWSRAQEEKEEKEKPR
                     LKNFDPRLRGQDGRKAEKGGAEEEDVVRVETNLVVADVLVLDKQGRSVAGLKREDFVV
                     REDNAPQKIGTFALGGGAGVPRSIVLVIDHSGSQLPYLKTSVEAAKTLVDRLGPRDRM
                     AVVTDSVELLADFTRDKALLKAKLDSLLQSTLDGRAGRSEHYSALLATLTELFDEEDL
                     RPVVIFQADGEELLALKGAKPRLRGVRERAFSFEDLLTAAEKSRATVYAIIPGLRFIG
                     LSQDEQLRRAQADMQNRMKATGELFGGAGRWPRHLPWSDWHYRRWADMTHRFQLAMAG
                     LAKYTGGWADYLERPEQARDVYARVFAGIESRYFIGYYSTNLARDGKRRKVSIEVRGH
                     PDYVVWGRKTYFAPGPDD"
     gene            complement(1978..>3336)
                     /locus_tag="AC159P_15365"
     CDS             complement(1978..>3336)
                     /locus_tag="AC159P_15365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HIR3696880.1"
                     /translation="GTGYELMAITAVVLGGASIFGGRGTVLGTVLGLFAIVILQNGLR
                     LSAQPAELAGILTGVLLVATILLDRLSRRAQKSAPRAAHTIAVEEESEVRNSQIGILS
                     AVVLGAALIVAGSNWLSVRSLREELRGGGGGAAGPQGSSGGGGGPAAGGPKKVIALMP
                     KAKGDPYFISCRQGAEEAAKELGVELLWDGPTDLDPAKQNEVVEAWITRGVDAIAVSV
                     ENKVAISTVLRKAKEKGIKIVTWDADSEKDARDFFINQATPQGIGYTLTDEAARIMGG
                     KGEFAIITASLSAANQNEWIKYIRERLAEKYPEMKLVAIQPSEGDRDRAFSETQTVLK
                     VYPNVKLVMPIAAPAVPGAAEAVKQSGRTDVKVTGLSLPNMSKPYVKEGVAESIVLWN
                     TLDLGYLTVYAANALSTGALKAGATEMNAGRLGRIEVVGDEVRLGAPFIFNRENIDRF
                     NF"
BASE COUNT          506 a         1143 c         1090 g          597 t
ORIGIN      
        1 gctccccctg aacgcacacg tgcgcccgag cgaggaagcc acaagagagg ggcaacggac
       61 ctttggtgcg agggccggtg gtttgtagaa agcccctgta aaccctgaca ggttgaatac
      121 gctgagcgtg atgcgcgggg cacagtttcg acgcgccgag ggggaattga tgagagggcg
      181 cgcgggagaa tcaggccctg tgggcagggc tgtccattcc ggtagcacgc ggtgagccgc
      241 gccgattgtc gattgtcgat tgtcgggagc gcgagaacat actccttttc cgccgacagt
      301 caatcgatat tttggttaca cgaaaagcgc gcgccggtcg tcgctcaatc gatgtggaac
      361 acctcgtcga gcgggagggc gacggggctg tcgtcggggt tggtgagcat caactctttc
      421 atgtgcgacc accagcggcg gcaggcgtcg gtcgcggcga tttgttgcca cagctcttcg
      481 gactcgacct cggcgtaggc gaacagctcg tcgcgccggc ggtcgaggaa gatcgagtag
      541 ttggagacgc cgtgactttt cagcacgtcg cgcagctcgg gccagatggg gttgtggcgc
      601 cgctcgtact cttcttgcac gcccggcctg agcgtcatca ggaatgcttt gcgaatcatc
      661 ttgggccact cacgaatcgg tcttcgtctt tacggcgacg cggcgcgagt cgccttcggg
      721 agagagtcag tcgtccggcc ccggcgcgaa gtaagtcttg cggccccaga cgacgtaatc
      781 ggggtggccg cgcacttcga tcgagacctt gcggcgcttg ccgtcgcgcg cgaggttggt
      841 cgagtagtag ccgatgaagt agcggctctc gatgccggcg aagacgcgcg cgtaaacgtc
      901 ccgtgcctgt tcgggacgtt cgaggtagtc ggcccatccg cccgtgtact tggcgagtcc
      961 ggccatcgcc aactggaagc ggtgtgtcat gtcggcccag cgcctgtagt gccagtccga
     1021 ccacggaaga tgcctcggcc accgccccgc cccgccgaac aactcgccgg tggccttcat
     1081 cctgttctgc atgtcggcct gcgcgcgccg gagctgctcg tcctgcgaca gcccgatgaa
     1141 gcgcagtccg gggatgatgg cgtagacggt ggcgcgcgac ttctcggcgg cggtcagcag
     1201 gtcttcgaag ctgaaggcgc gctcgcgcac gccgcgcaag cggggcttcg cccccttcag
     1261 cgcgagcaac tcctcgccgt ccgcctggaa gatgacgacg gggcggaggt cttcctcgtc
     1321 gaacaactcg gtgagcgtcg ccagcaacgc gctgtagtgt tcgctgcgcc cggcgcgccc
     1381 gtcgagcgtg ctttgcagca gcgagtcgag cttcgccttc aagagcgcct tgtcgcgcgt
     1441 gaagtccgcg agcagctcga cgctgtcggt cacgaccgcc atccggtcgc gcgggccgag
     1501 cctatcgacg agcgtcttcg ccgcctcgac gctcgtcttg agatacggca gctggctgcc
     1561 gctgtggtcg atgacgagga cgatggagcg cggcacgccc gcgccgccgc cgagcgcgaa
     1621 ggtgccgatc ttctgcggcg cgttgtcttc cctgacgacg aagtcttcac gcttcagccc
     1681 cgcgaccgag cgcccctgct tgtcgagcac cagcacgtcc gcgacgacga ggttcgtctc
     1741 gacgcgaacc acgtcctctt cctcggcgcc gcccttctcc gccttccgtc cgtcctgccc
     1801 tctgagccgc gggtcgaaat tcttcagccg cggcttctcc ttctcttcct tctcctcctg
     1861 cgcgcgagac cacgccgccg cgtcggcgcc cgcgaagaag aagacgccga cgacgcacgt
     1921 gatgacgaag gccagcgaca gaggcgagac gtgtttcatc gcgcgagccg taacgagtca
     1981 gaagttgaag cggtcgatgt tctcgcggtt gaagatgaac ggggcgccga gccgtacctc
     2041 gtcgccgacg acttcgatcc tgccgagccg cccggcgttc atctcggtgg ccccggcctt
     2101 gagcgcgccg gtcgagagcg cgttggccgc gtagaccgtc aggtagccga ggtcgagcgt
     2161 gttccacagc acgatggatt cggccacgcc ctccttgacg tagggcttcg acatgttcgg
     2221 cagagagagg cccgtcacct tcacgtccgt tcggcccgac tgcttgacgg cctcggcggc
     2281 gccgggcacg gcgggcgcgg caatcggcat gacgagcttc acgttcgggt agaccttcag
     2341 gacggtctgc gtctcggaga acgcgcggtc gcggtcgcct tcggagggct ggatcgccac
     2401 gagcttcatc tccgggtact tctccgccag ccgctcgcgg atgtacttga tccactcgtt
     2461 ctggttggcc gcgctcaaac tggcggtgat gatggcgaac tcgcccttgc cgcccatgat
     2521 gcgcgcggcc tcgtccgtca gcgtgtagcc gatgccctgc ggcgtcgctt ggttgatgaa
     2581 gaagtcgcgc gcgtccttct cggagtcggc gtcccacgtg acgatcttga tgcccttctc
     2641 cttcgccttg cggagcacgg tcgagatggc gaccttgttc tcgacgctga cggcgatggc
     2701 gtccacgccg cgcgtgatcc acgcctcgac cacttcgttc tgcttcgccg ggtcgaggtc
     2761 ggtcgggccg tcccacagca gctcgacgcc cagctccttc gcggcctctt cggcgccctg
     2821 ccggcaactg atgaagtacg ggtcgccctt cgccttcggc atcagcgcga tgaccttctt
     2881 cgggccgccc gccgcggggc cgccgccgcc gcccgaagac ccttgcggcc ccgccgcccc
     2941 gccgccgccg ccgcgcaact cctcgcgcag cgagcgcacc gagagccaat tgctcccggc
     3001 gacgatcaac gcggcgccga ggacgaccgc gctcaggatg ccgatctgtg agttcctcac
     3061 ttcgctctcc tcctctacag cgattgtgtg tgcggcgcgc ggcgcgctct tctgcgcgcg
     3121 acgcgacagg cggtcgagca aaatcgtcgc cacgagcagc acgcccgtca ggatgcccgc
     3181 cagctccgcc ggctgcgcgc tcagccgcag cccgttctgc aaaatcacga tggcgaacag
     3241 cccgagcacc gtgcccagca ccgtcccgcg cccgccgaag atcgacgcgc cgcccaagac
     3301 caccgccgtg atcgccatca actcgtagcc ggtgcc
//
LOCUS       DBNGOH010000800         4210 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3970657, whole genome shotgun sequence.
ACCESSION   DBNGOH010000800 DBNGOH010000000
VERSION     DBNGOH010000800.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4210)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4210)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4210
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3970657"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /locus_tag="AC159P_15370"
     CDS             complement(<1..218)
                     /locus_tag="AC159P_15370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012596831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3696881.1"
                     /translation="MRITFLGTSAGVPTRARNVSCVALRLEQRGEVWLFDCGEGTQHQ
                     LLRSDLNISQITRILITHLHGDHLYGLMG"
     gene            complement(393..1037)
                     /locus_tag="AC159P_15375"
     CDS             complement(393..1037)
                     /locus_tag="AC159P_15375"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HIR3696882.1"
                     /translation="MSSKNSSSSAARRARGERLGRDFYTRADTLLVARELLGKRLVVP
                     APTGERVSGRVVETEAYVGIEDMASHAYGGRRTARTETMYAVGGTAYVYFVYGMHHQF
                     NAVTGERDSPHAVIVRALEPEEGVEWMRERRPSKKDRELTSGPGKLCRALGIDMTFNR
                     ADLTGPHVWLEDAGAVVAPADIASGPRVGIDYAGDYVSKPWRFWLKGNPYVSKQ"
     gene            complement(1012..1674)
                     /locus_tag="AC159P_15380"
     CDS             complement(1012..1674)
                     /locus_tag="AC159P_15380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HIR3696883.1"
                     /translation="MDATLDEVEVRVLGALVEKQLATPDYYPLTLNALVNACNQISNR
                     DPVVAYDERTVERAVDSLRGKNLAYVFYGSESRVPKYKHVMTEIFRLSPPELAAMCVL
                     MLRGPQTVGEVRGRTGRLHNFADLSEVEATLDGLARREEPLVTKLPRQAGRKEARYAQ
                     LLGGMPAVEEEAEEPRGGARESRAGGGGDRVARLEAEVEALRGELAELRQRFDEFEKQ
                     FE"
     gene            complement(1925..2935)
                     /locus_tag="AC159P_15385"
     CDS             complement(1925..2935)
                     /locus_tag="AC159P_15385"
                     /inference="COORDINATES: protein motif:HMM:NF016289.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HIR3696884.1"
                     /translation="MNKLLLIITLLTTGAAALPAVVACSQAVQKADTQAQKAAPGGQS
                     QTVTSKIIDAAQLLEDVRTLSSDAMEGRKFGTPGGAKARAYVVRRFAEVGLKPFFGAS
                     FEQPVSHKTGEQTIEGANVVGVVRGKSNPERFLVVTAHYDHLGVRDGKTYNGADDNAS
                     GVAVLLQMAAHFAKQQPANSIIFAALDAEEGSGFAGSRTLVKSLLDAKRNVALNVNLD
                     MVSHSDRGELYASGLYHYPALKPVVERVAARAPVKLLTGHDRPEQGTNDWTTQSDHMA
                     FHERKITFLYFGVEDHKDYHQPTDDFETITPDFFVRAAETILDVVKTLDAEPAGGEKK
                     GK"
     gene            complement(2962..3900)
                     /locus_tag="AC159P_15390"
     CDS             complement(2962..3900)
                     /locus_tag="AC159P_15390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017744513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apurinic/apyrimidinic endonuclease family
                     protein"
                     /protein_id="HIR3696885.1"
                     /translation="MGDKVGAGTDGVPQLGLVCITHTDAVRYRALTRKRLLQFGADEQ
                     KRVLRELYADNLRRLNGAIDFCAARGWRLYRMTSGLFPFADDEAGEDVLEEFRAELAR
                     TGRRATAFGLRLVLHPDQFVVLSSDSPQTIANSIKILDAHARVMDLLEQPRSPWALIE
                     IHGGKGGRSERLVDVIGQLPEAVRARIAFENDEYIYSAAEILDVCRRARVPMVFDAHH
                     HVVHEGLTSYDHPSVGEMVDAARETWPVPAWQLAHISNGRERFGDRQHSDLVTEMPSS
                     YRRVPWIEVEAKHKELAVEKLRAEWAAGGATKATVA"
     gene            complement(3915..>4210)
                     /locus_tag="AC159P_15395"
     CDS             complement(3915..>4210)
                     /locus_tag="AC159P_15395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696886.1"
                     /translation="VFYTASAAPRVRPDAWVRRWSSAALALLVIQLVAGMTNVALLAP
                     VWLQLVHLLLADLLWVALVLLAAATLAEATEDSKAFEQSGVTAAATTAASAEL"
BASE COUNT          622 a         1418 c         1418 g          752 t
ORIGIN      
        1 cccatcagcc cgtagaggtg gtcgccgtgc aggtgcgtga tgaggatgcg cgtgatctgg
       61 ctgatgttca ggtcgctacg cagcagttgg tgctgcgtgc cctcgccgca gtcgaagagc
      121 cacacctcgc cgcgctgctc aagccgcagc gccacgcacg acacgttgcg cgcgcgcgtc
      181 ggcacgcccg cgctggttcc gagaaaagtg attcgcatct ttgaaagtga gcagtgagct
      241 gtaagctgta agcagaagtt tgaagatgct tgagaggtaa agcgctaagc cccctccgtc
      301 tactgcctcg tctgctatta accactcacc gcttgccgct cagcactttc tgcctactgc
      361 ctactgtctt cttactgctc tctgctcacc gctcactgct tactgacgta tgggttgcct
      421 ttgagccaga agcgccaggg cttcgagacg tagtcgcccg cgtagtcgat gccgacgcgg
      481 gggccggagg cgatgtctgc gggcgcgacc acggcccccg cgtcttcgag ccagacgtgc
      541 gggccggtga ggtctgcacg gttgaaggtc atgtcgatgc cgagcgcgcg gcagagcttg
      601 ccggggccgc tcgtgagttc cctgtccttc ttcgacggtc gtcgctcgcg catccactcg
      661 acgccctctt cgggttcgag cgcgcggacg atgacggcgt gcggcgagtc gcgctcgccg
      721 gtgacggcgt tgaactggtg gtgcatgccg tagacaaagt agacgtaggc cgtgccgccg
      781 accgcgtaca tcgtctcggt gcgcgccgtg cgtcgcccgc cgtaggcgtg cgaggccatg
      841 tcttcgatgc cgacgtaggc ttcggtctcg acgacgcggc ccgacacgcg ctcgcccgtc
      901 ggcgcgggca cgacgaggcg cttgccgagc agttcgcgcg cgaccaagag cgtgtcggcg
      961 cgcgtgtaaa aatcacgccc gagcctctcg ccgcgcgcgc gccgcgccgc gctactcgaa
     1021 ctgtttttcg aactcatcga acctctgcct cagctcggcc agctcgccgc gcagcgcctc
     1081 gacctccgct tcgagccgcg ccacgcggtc gccgcccccg cccgcgcgcg actcgcgcgc
     1141 gccgccgcgc ggctcctcgg cctcctcctc caccgccggc atcccgccga gcagttgcgc
     1201 gtagcgcgcc tccttgcgcc ccgcctgtcg cgggagcttc gtgacgagcg gctcttccct
     1261 tcgcgccagc ccgtcgagcg tcgcctcgac ctcggagagg tccgcgaagt tgtggagcct
     1321 gccggtgcgc ccgcgcacct cgccaacggt ctgcggcccg cgcagcatga gcacacacat
     1381 cgccgccagc tccggcggcg agaggcggaa gatttccgtc atcacgtgct tgtacttcgg
     1441 cacgcggctc tcgctgccgt agaagacgta ggcgagattc ttcccgcgca gagaatcgac
     1501 cgcgcgctcg accgtccgct cgtcgtaagc gacgaccggg tcgcggttcg atatctggtt
     1561 gcacgcgttc accagcgcgt tcagcgtcag cgggtaataa tcgggcgtcg ccaactgctt
     1621 ctcgaccagc gcgccgagca cgcgcacctc cacttcgtcc agcgtcgcgt ccatgcgttt
     1681 aatctacttc atccggcgcc gatttgcgaa agcagcgggc agcgggcagc gggcagcagg
     1741 cagaaaggaa agtagccagt aggcggtagg cagtaggcag tcaagacaga agaagagcga
     1801 agagcttgag aggtaaagtg caaagcctct ctatggccgc ttgatgaacg aagaggcgtt
     1861 gcactttacc tctcaagctt cttcgtgctt ctgtttttac tgcctgctgc ccgctgcctg
     1921 ctgcctactt ccctttcttc tcgccgcccg cgggttcggc gtcgagcgtc ttgacgacgt
     1981 cgaggatggt ctcggcggcg cggacgaaga agtcgggggt gatggtctcg aagtcgtccg
     2041 tgggctggtg gtagtccttg tggtcttcga cgccgaagta gaggaaggtg atcttccgtt
     2101 cgtggaaggc catgtggtcg gactgcgtcg tccagtcgtt cgtgccctgc tcgggccggt
     2161 cgtggcccgt caggagtttg acgggcgcac gcgcggcgac gcgctcgacg acgggtttga
     2221 gcgcggggta gtggtagagg ccggaggcgt agagttcgcc gcggtcgctg tggctgacca
     2281 tgtcgaggtt gacgttgagg gcgacgttgc gtttggcgtc gaggagcgac ttgacgagcg
     2341 tgcgcgagcc ggcgaagccg ctcccctctt cggcgtcgag cgcggcgaag atgatggagt
     2401 tcgcgggctg ttgtttggcg aagtgcgcgg ccatctggag cagcacggcg acgcccgagg
     2461 cgttgtcgtc ggccccgttg taggtcttgc cgtccctgac gccgaggtgg tcgtagtgcg
     2521 ccgtcacgac gaggaagcgc tcggggttcg acttgccgcg gacgacgccg acgacgttcg
     2581 ccccctcgat cgtctgctcg cccgtcttgt ggctgacggg ctgctcgaac gaggcgccga
     2641 agaaaggctt cagcccgact tccgcgaacc tgcggacgac gtaggcgcgc gccttcgcgc
     2701 cgcccggcgt gccgaacttg cgcccctcca tcgcgtcgct cgacagcgtg cgcacgtctt
     2761 cgaggagctg cgcggcgtcg atgattttgg aggtgacggt ctgcgactgg ccgccgggcg
     2821 cggccttctg cgcctgcgtg tcggcctttt gcacggcctg cgagcaggcg acgaccgccg
     2881 gaagcgcggc ggcgcccgtc gtcaacagcg tgatgatgag aagtagtttg ttcatgattt
     2941 catgccgacg ccgccgggga ctcaagccac cgtcgccttc gtcgcgcctc cggcagccca
     3001 ttcggcgcgc agcttctcga cggccagctc cttgtgcttg gcctcgacct cgatccaggg
     3061 cacgcggcgg taagacgagg gcatctcggt gacgaggtcg ctgtgttggc ggtcgccgaa
     3121 gcgctcgcgc ccgttcgaga tgtgtgcgag ctgccacgcg gggacgggcc acgtctcgcg
     3181 cgcggcgtcc accatctcgc cgacgctcgg gtggtcgtaa gacgtgagac cttcgtggac
     3241 gacgtggtgg tgcgcgtcga agaccatcgg cacgcgcgcg cggcggcaca cgtcgagaat
     3301 ctccgcggcg ctgtagatgt actcgtcgtt ctcgaacgcg atgcgcgcgc gcacggcctc
     3361 gggcaactgg ccgatgacat cgacgaggcg ttcgctgcgc ccgcccttgc cgccgtggat
     3421 ttcgatcaag gcccacggcg agcgcggctg ttcgagcaag tccatcacgc gcgcgtgcgc
     3481 gtcgagaatc ttgatgctgt tggcgattgt ctgcggcgag tcggagctga ggacgacgaa
     3541 ctgatccggg tggagcacga gccgcaagcc gaacgcggtc gcgcgccttc cggtgcgcgc
     3601 gagttccgcg cggaactctt cgagcacgtc ctcgcccgcc tcgtcgtccg cgaacgggaa
     3661 cagccccgag gtcatgcggt agaggcgcca gccccgcgcc gcgcagaagt cgatcgcgcc
     3721 gttcagccgc ctgaggttgt ccgcgtacag ttcgcgcagc acgcgcttct gctcgtcggc
     3781 gccgaactgc aacaggcgct tgcgcgtcag cgcgcgatac ctcaccgcgt ccgtgtgcgt
     3841 gatgcagacc agaccgagct gcggcacgcc gtccgtcccc gcgcccacct tgtcgcccat
     3901 ctgaagcccc ctcgttataa ctccgccgac gcggcggtcg tcgccgccgc tgtcacgccc
     3961 gactgctcga acgccttcga gtcctccgtt gcctcggcca gcgtcgcggc ggcgagtagc
     4021 acgagcgcca cccacagcaa gtccgcgagc agcaggtgga cgagctggag ccagaccggc
     4081 gccagcagcg ccacgttcgt catcccggcg acgagttgaa tgacgagcag cgccagcgcc
     4141 gccgacgacc agcgcctcac ccacgcgtcg gggcgcacgc gcggcgccgc gctcgccgtg
     4201 tagaagacga
//
LOCUS       DBNGOH010000801         3597 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_120294, whole genome shotgun sequence.
ACCESSION   DBNGOH010000801 DBNGOH010000000
VERSION     DBNGOH010000801.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3597)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3597)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3597
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_120294"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3597)
                     /locus_tag="AC159P_15400"
     CDS             complement(<1..>3597)
                     /locus_tag="AC159P_15400"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3696887.1"
                     /translation="ALREVELWRHFLRRFEGGEEKFVHWSFWPSTVFTADAARALTAL
                     FASDAELQQIMRRTKIWATEEVILPTLLALLGFGILANPCSDDYLKFRVRFTPRQLDA
                     ALAREDVFWVHPVARNDRDGLRRHIRARLNQYEKPFEEGAVMPTNDAKPSAGHDHNLL
                     LTLPILERMKTVEGWLWEDEADLLIAATSRALRDLPAPHKIVEVGSYCGRSTVVFGSV
                     VKALRPDAKVYAIDPHDGCVGATDQRITKTAPTLEKFQWNIRNAGLTETVELIQKYSH
                     EVEWHDPISLLFIDGLHDYTNVARDFFHFEHWVVEGGYVAFHDYADYYPGVKTFVNEV
                     LASGRYRQVHCAMSMIVVQKLRAAESSSDAGNRQQQQLAEQHAAGTSAAAHVAEPPIA
                     AQPPAATAAAAQHAAAAQRAAVQPASASAHAAPIPKVPRGERFDRLSAFRGVHEGESV
                     IVCGCGESLNELERPEEFLTVGVNDVGRRFQPNYLVVVNPRNQFKGDRFRYVEQSRAD
                     YLFTQLSLGLPNRNVIRFRLGSYGGTDFANPDVLHYTQNSPYVALCLAAHMGARRVGL
                     IGVDFTDHHFFARTGRHALAAQLKTIDEQYRRLGAALAARGIEVFNLSRASRLTAFPK
                     LSIDEFAARSNHKPEAEESAPLKIVSYATTPVAGVPAILARCIKTKTPHAARCVWAAS
                     GYGNGVTFDGDIEWSHSPAKANEELESADVVVVHNGKVEPRHRKLVAGKAVVTMAHNY
                     LWNVDQTYVKQGFPGVVVGQYQATLAEFAGWSAVPNPVPLWEPQFQPGDKGEAVTVCY
                     TPSGKHERYAPGHRLYWHSKGYDTTMRVLGRLAARYAIRLEVIRDRQVSHAESLAMKR
                     RSHVVIDECVTGSYHRNSLEGLSAGCVVVNGVGLLPAVREVFRLCSGDDANPFVFANL
                     EGLEGALCALVERGAVALAADGAQGRLWMERRWDFARQWETFWSPVIDRALRHAGRKT
                     RSLEPAAHAATSHEARPSRAPAHEGVAAQATARPETSVQDVALARPAANAAGAEAELS
                     AQLARASTRGTVSVVIPHGGRDRLRNLTATLSNLRACPDVGEVIVVDTGAAPTAEGVA
                     RRLAEKYLFVRHDGLFNRARALNAGAALAECEHLLWQDNDLISAPDFLKKALEELAAR
                     SLDCLLPWTSIRYLSAGDSVEVCQGARRAADCRPAHAWTNRHAVGGAMLVRK"
BASE COUNT          515 a         1169 c         1313 g          600 t
ORIGIN      
        1 tctttgcgga cgagcatcgc gccgccgacc gcgtggcgat tcgtccaggc gtgtgcgggg
       61 cgacagtccg ccgcgcggcg cgcgccctga cagacctcga cgctgtcgcc cgccgaaagg
      121 tatcgaatcg aagtccacgg gagcaggcag tccagactgc gcgcggccaa ctcttccagc
      181 gccttcttca gaaagtccgg cgccgagatg aggtcgttgt cctgccagag caggtgctcg
      241 cactcggcga gggccgcgcc cgcgttcagc gcgcgcgcgc ggttgaacag cccgtcgtgg
      301 cggacgaaca gatacttctc cgccagacga cgcgcgaccc cttctgccgt cggcgcggcg
      361 cccgtatcga cgacgatcac ctcgccgaca tcagggcacg cgcgcaggtt cgagagcgtc
      421 gccgtcaggt tgcgcaggcg gtcgcgcccc ccgtgcggga tgacgacgct caccgtgcct
      481 cgcgtgctcg cgcgcgcgag ctgtgcggag agttcggcct cggcgcccgc cgcgttggcc
      541 gcgggccgtg cgagcgcaac gtcttgcacc gaggtctcgg gccgggcggt cgcctgcgcc
      601 gcgacccctt cgtgagcggg cgcgcgcgaa ggtcgagctt catgcgaagt ggccgcgtgt
      661 gcggcgggtt cgagcgaacg ggttttcctt cctgcgtgcc gcaaagctct gtcgatgacg
      721 ggcgaccaga acgtctccca ctgtcgggcg aagtcccagc ggcgctccat ccagaggcgg
      781 ccctgcgcgc cgtcggcggc gagcgccacg gcgccgcgct cgacgagcgc acagagcgcg
      841 ccctccaatc cttcgagatt cgcgaagacg aacgggttcg cgtcgtcgcc cgagcagagg
      901 cggaacactt cgcggacggc ggggagcaag cccactccgt tgacgaccac gcaccccgcg
      961 ctcaagcctt cgaggctgtt gcggtggtag ctgcccgtca cgcattcgtc gatgacgacg
     1021 tgcgaccggc gcttcatcgc cagcgactcg gcgtgcgaga cctgccggtc gcggatcacc
     1081 tccaaccgaa tcgcgtagcg cgcggccagc cgtccgagca cgcgcatcgt cgtgtcgtag
     1141 cccttcgagt gccagtagag gcggtggccc ggcgcgtagc gctcgtgctt gccggagggc
     1201 gtgtagcaga cggtgacggc ctcgcccttg tcgccgggtt ggaactgcgg ctcccacagc
     1261 ggcacggggt tggggacggc tgaccagccc gcgaactccg cgagcgtcgc ctgatactgt
     1321 ccgaccacga cgccggggaa gccctgcttc acgtacgtct gatcgacgtt ccagaggtag
     1381 ttgtgcgcca tcgtcacgac ggccttgccc gcgacgagct tgcggtggcg cggctcgacc
     1441 ttgccgttgt ggacgacgac cacgtccgcg ctctcaagct cttcattcgc cttcgcgggc
     1501 gagtgcgacc actcgatgtc gccgtcgaag gtgacgccgt tgccgtagcc gctcgcggcc
     1561 cagacgcagc gcgcggcgtg cggcgtcttc gtcttgatgc agcgcgcgag gatcgcgggg
     1621 acgcccgcca cgggcgtcgt cgcgtaggaa acgatcttga gcggcgcgct ctcttcggct
     1681 tcgggcttgt gattggagcg ggccgcgaac tcgtcgatgg agagtttggg gaacgccgtg
     1741 aggcggctcg cgcggctgag gttgaagacc tcgatgccgc gcgccgcgag cgccgcgccg
     1801 aggcggcggt actgctcgtc gatggtcttg agctgcgccg cgagggcgtg cctgccggtg
     1861 cgcgcgaaga agtggtggtc ggtgaaatcg acgccgatca ggccgacgcg ccgggcgccc
     1921 atgtgcgcgg cgaggcagag cgcgacgtag ggcgagttct gcgtgtagtg cagcacgtcc
     1981 ggattcgcga agtccgtgcc gccgtaggag ccgaggcgga agcggatgac gttgcggttc
     2041 ggcaggccga ggctcaattg cgtgaagagg tagtcggcgc gcgactgctc gacgtagcgg
     2101 aagcggtcgc ccttgaactg gttgcgcggg ttgacgacga cgaggtagtt gggttgaaag
     2161 cgccggccca cgtcgttgac gccgacggtg agaaactctt cggggcgctc aagctcgttc
     2221 agagactcgc cgcacccgca gacgatgacg ctctcgcctt cgtgaacgcc gcggaaggcc
     2281 gagagcctgt cgaagcgctc gccgcggggg actttcggga tgggcgccgc gtgtgcgctc
     2341 gcgctcgccg gttgaactgc cgcgcgttgt gcggcggctg cgtgttgtgc ggcggcggct
     2401 gttgcggcgg gtggctgcgc ggcgatgggc ggctcggcga cgtgcgccgc ggcgctcgtc
     2461 ccggcggcgt gttgttcggc gagctgttgc tgctgccggt tgcccgcgtc gctggaggat
     2521 tcggcggcgc gcagtttctg cacgacgatc atgctcatcg cgcagtggac ttggcggtag
     2581 cgaccggagg cgagcacctc gttgacgaag gtcttcacgc cggggtaata gtcggcgtag
     2641 tcgtggaagg cgacgtagcc gccctcgacg acccagtgct cgaagtggaa gaagtcgcgc
     2701 gcgacgttcg tgtagtcgtg tagcccgtcg atgaagagca ggctgatggg gtcgtgccac
     2761 tcgacctcgt gcgagtactt ctggatcagc tcgaccgtct ccgtcagccc cgcgttccgg
     2821 atgttccact gaaacttttc gagcgtcggg gcggtcttcg tgatgcgctg gtcggtcgcg
     2881 ccgacgcatc cgtcgtgcgg gtcgatggcg tagaccttcg cgtcggggcg gagcgccttg
     2941 acgacgctgc cgaagacgac cgtcgagcgc ccgcagtagc tgccgacctc gacgatcttg
     3001 tgcggcgcgg gcaaatctcg gagggcgcgc gaggtggcgg cgatcaaaag atctgcctcg
     3061 tcctcccaca gccagccctc gaccgtcttc atgcgttcga ggatgggcag cgtcagcagc
     3121 aggttgtggt cgtggccggc ggatggtttg gcgtcgttgg tcggcataac cgctccctcc
     3181 tcgaacggct tctcgtactg gttgagtctg gcgcggatgt gcctgcgcag gccgtcgcgg
     3241 tcgttgcgcg cgaccgggtg cacccagaac acgtcctcgc gcgcgagcgc cgcgtcgagc
     3301 tgccgcggcg tgaagcggac gcggaacttg aggtagtcgt cgctgcacgg gttggcgagg
     3361 atgccgaaac cgaggagcgc cagcagcgtc ggcaggatga cctcttcggt cgcccagatt
     3421 ttcgtccgcc gcatgatctg ctgaagctcc gcgtccgagg cgaagagcgc ggtcagcgcg
     3481 cgcgccgcgt ccgccgtgaa gacggtcgag ggccagaaag accagtggac gaacttctcc
     3541 tcgccgccct cgaagcggcg caggaagtgg cgccacagct cgacctccct gagcgcg
//
LOCUS       DBNGOH010000802         4698 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3053886, whole genome shotgun sequence.
ACCESSION   DBNGOH010000802 DBNGOH010000000
VERSION     DBNGOH010000802.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4698)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4698)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4698
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3053886"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3155
                     /locus_tag="AC159P_15405"
     CDS             <1..3155
                     /locus_tag="AC159P_15405"
                     /inference="COORDINATES: protein motif:HMM:NF012862.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HIR3696888.1"
                     /translation="AVRLWDAGTGALVRALDEAAKKEDVRVIAFSPDGRTLAASSDKL
                     KLWDVATGRLVRDFEEDVYAVAFSPDGKLVAGVTLGRAVKLWEAGTGRLARALEANAY
                     LSPEAISFSPDGATLAVVTYDGSVDLWDMARASVRRTLKSPDYREGMDIVRFSPDGGR
                     LFVSNAPHIGRIQRSLDLWDVAANEIVTKIDGRNIIIDSVAFSPDGKTFIAVGSGERS
                     GQHLIRLWDAASGEVVRRFGTWQGGVTSLAVNAERKSVVMAAEKAPTKFYDLGAGPIC
                     ALERTRELRSVAFGPEGRTVVLGDPSNSSLSFWDAGDCRRIGEFAERRFAFSHDGKAI
                     AEWHRSKAVNLYDAARLRVTLSLRGHAGDIEDFAFSPDGRHGASASIDGTARLWELGT
                     GQSLRAFEGHAGGATAVAFSADNRVLATAGRDGAVKLWDAATGRPLHTLAGHTRAVAA
                     LAFDSTGKLFASCDVEGGVRLWDAKSGAALRTLGSFPRGARTVFFGPDGKTVAALGLE
                     PNEYGNAPADLTVFKLWDAATGRLILDLKGLEVKSASFSRGGRAVLVAAGLPYKGTTT
                     KVYSAATGEVLADVIGFGDGSWVVVTPEGFFDGPPAAWNQIAWRFAGNRIAPVEIFFN
                     EFYRPGLLAEVLDGAPPKAPRAFAQLDRRQPRLRLSRADAAKPGDEIKTRTLKVKIDI
                     DEAPAGGRDVRLFRNGSLVRVWRGDVLKGRAAALETEITVEAGENVLTAYAFNNDDIK
                     SADVTLELKGASALARKGVARLVAVGVNAYANPRYDLKYAVADAVAFGEEFKRQQERL
                     GRYERVEVKTLTDRQATKAGILRALEELSAVAEPEDAVVVFFAGHGTARDSRFYLLPH
                     DLGYAGAPSALDRAGLEAMLAHGVSDRELERAFEKIDAARLLFVIDACNSGQALEAAE
                     RRRGPMNSKGLAQLAYEKGMYILTASQSYEAAQEASQLGHGLLTYALVEEGLKRAAAD
                     TEPKDGTVHVKEWLDFAARRVPEIQLEEMSRAATSGRDLSFAEEERGITLARRQGQRP
                     RLYYRRELEARPLVVAKTRN"
     gene            3258..3458
                     /locus_tag="AC159P_15410"
     CDS             3258..3458
                     /locus_tag="AC159P_15410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696889.1"
                     /translation="MNDGTCWNCGHPHAHHTILGGCSYEREGVRCDCPRYEDSIYHGE
                     QRKRDTYTLSDRRRLGLGRGRR"
     gene            3520..3810
                     /locus_tag="AC159P_15415"
     CDS             3520..3810
                     /locus_tag="AC159P_15415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696890.1"
                     /translation="MTGEEMERAIEFLLQNHATHEERLRQTQQQIAETNRIMQMHAET
                     QTEFIKIVTKAIGDLAEALQRTAEAQRRTDDRVDRLAATVERFISEGRDGKV"
     gene            complement(3837..4088)
                     /locus_tag="AC159P_15420"
     CDS             complement(3837..4088)
                     /locus_tag="AC159P_15420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696891.1"
                     /translation="MLGFSAHGKATFEVTRTEMEMGDRPLDDNRPPAPAPPRDRQPRG
                     GYYYDDRTGYEIYNPADEDEDEKQEGGEARRENTTGARD"
     gene            4173..>4698
                     /locus_tag="AC159P_15425"
     CDS             4173..>4698
                     /locus_tag="AC159P_15425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolB family protein"
                     /protein_id="HIR3696892.1"
                     /translation="MCVRAAAACVVVLALLSGGSVAVARRQSPQARAQRFELTVDSIM
                     RGPELVGHPPSGVVWSQDSGRVFFRWKRAGEPRLKAMDWYVVNRDGTGLRKLSEEEAR
                     RAGPPPASGELSKDKTLTVFTEDGDVFLYDHAKGERRQVTRTVDAETNAHFTRDQKRV
                     YFTRQNNLYVMSLDG"
BASE COUNT          795 a         1593 c         1630 g          680 t
ORIGIN      
        1 acgcggtcag gctgtgggac gccgggacgg gcgcgctcgt gcgcgcgctg gacgaggcgg
       61 cgaagaaaga ggacgtgcgc gtgatcgcgt tcagccccga cggcaggacg ctcgccgctt
      121 cgagcgacaa gctgaagctg tgggacgtgg cgacggggcg gctcgtgcgc gatttcgaag
      181 aagacgtgta cgcggtcgcg ttcagccccg acggcaaact cgtcgcgggc gtcacgctcg
      241 ggcgcgccgt caagctgtgg gaggcgggga cgggccgcct cgcccgcgcc ctcgaagcca
      301 acgcctacct cagccccgaa gcgatctctt tcagtcccga cggcgcgacg ctcgcggtcg
      361 tcacctacga cgggtcggtc gatctatggg acatggcgcg cgcgagcgtg aggcgcacgc
      421 tgaagtcgcc cgactaccgc gagggcatgg acatcgtccg gttcagcccc gacggcgggc
      481 ggctcttcgt ctcgaacgcg ccgcacatcg ggcgcatcca gcggagcctc gacctgtggg
      541 acgtggcggc gaacgagatc gtgacgaaga tcgacggcag gaacatcatc atcgactccg
      601 tcgccttcag ccccgacggc aagaccttca tcgcggtcgg ctcgggcgag cgctcgggcc
      661 agcacctcat tcgcctctgg gacgccgcga gcggcgaggt cgtccgcagg ttcgggacgt
      721 ggcagggcgg cgtcacctcg ctcgccgtca acgccgagcg gaagagcgtc gtgatggccg
      781 ccgagaaggc gccgacgaag ttctacgacc tcggcgcggg gccgatctgc gcgctcgaac
      841 gcacgcgcga actgcgctcc gtcgccttcg gccccgaagg gagaacggtc gtgctcggcg
      901 acccttcaaa cagctctctg agcttctggg acgcgggcga ctgccgccgg atcggcgagt
      961 tcgccgagcg gcgtttcgcc ttcagtcacg acggcaaagc aatcgccgag tggcatcgga
     1021 gcaaggccgt caacctctac gacgccgcgc ggctccgcgt gacgctcagc ctgcgcgggc
     1081 acgcgggcga catcgaagac ttcgccttca gccccgacgg caggcacggc gcgtccgcga
     1141 gcatcgacgg cacggcgcgg ctgtgggaac tcggcaccgg ccaatccttg cgcgccttcg
     1201 aaggccacgc gggcggcgcg accgccgtcg ccttctcggc ggacaaccgc gtcctcgcca
     1261 cggcgggcag ggacggcgcc gtgaaactct gggacgcggc gacgggccgc ccgctccaca
     1321 ccctcgccgg tcacacacgc gccgtcgcgg cgctcgcctt cgattcaacg gggaagctct
     1381 tcgcgtcgtg cgacgttgaa ggcggcgtgc gtctgtggga tgcgaagagc ggcgccgcct
     1441 tgcgcacgct cggcagcttc ccgcgcggcg cgcgcacggt cttcttcggc cccgacggaa
     1501 agacggtcgc cgcgctcggc cttgagccga acgagtacgg caacgcgccc gccgatctca
     1561 ccgtcttcaa actctgggac gcggcgacgg gccgcctgat tctcgacctc aaaggcttgg
     1621 aggtgaagtc cgcttccttc agccgcggcg gcagggcggt gctcgtcgcc gcgggcttgc
     1681 cttacaaggg gacgacgacg aaggtctact ccgccgcgac gggcgaagtg ctcgcggacg
     1741 tgatcggctt cggcgacggc tcgtgggtcg tcgtcacgcc cgaaggcttc ttcgacggcc
     1801 cgcccgccgc ctggaatcaa atcgcgtggc gcttcgccgg caacagaatc gcgcccgtcg
     1861 aaatcttctt caacgagttc taccgccccg gcctgctcgc ggaagttctg gacggcgcgc
     1921 ccccgaaagc gccgcgcgcc ttcgcgcagc tcgaccgccg ccagccgcgg ctgcgcctct
     1981 cgcgcgccga cgccgcgaag cccggcgacg agataaagac gcgcacgctg aaggtcaaga
     2041 tcgacatcga cgaagcgccc gcgggcgggc gcgacgtgcg gctgttccgc aacggctcgc
     2101 tcgtccgcgt ctggcgcggc gacgtgctca agggtcgcgc ggcggcgctc gaaacggaga
     2161 tcaccgtcga ggccggcgag aacgtgctca ccgcctacgc cttcaacaac gacgacatca
     2221 agagcgccga cgtgacgctc gaactcaagg gcgcgtccgc cctcgcgcgc aagggcgtcg
     2281 cgcgcctcgt cgccgtcggc gtcaacgctt acgcgaaccc gcgctacgat ctgaagtacg
     2341 ccgtggccga cgccgtcgcc ttcggcgaag agttcaagcg gcagcaggaa cgcctcgggc
     2401 gctacgagcg cgtcgaggtg aagacgctca ccgacaggca ggcgacgaag gcgggcatcc
     2461 tccgcgcgct cgaagagttg tccgcggtgg ccgagccgga agacgccgtc gtcgtcttct
     2521 tcgcgggcca cggcacggcg cgcgacagtc gcttctacct gctgccgcac gacctcggct
     2581 acgcgggcgc gccctccgca ctcgaccgcg cgggcctcga agcgatgctc gcgcacggcg
     2641 tctccgaccg cgagctggaa cgcgccttcg agaagatcga cgccgcgcgc ctcctcttcg
     2701 tcatcgatgc ctgcaactcc gggcaggcgc tcgaagcggc ggagcgccgc cgcggcccga
     2761 tgaactcgaa gggcctcgcg cagctcgcct acgagaaagg catgtacatc ctcaccgcct
     2821 cgcagagcta cgaggccgcg caggaggctt cgcagctcgg ccacggcctt ttgacttacg
     2881 cgctcgtcga agagggcctg aagcgcgccg ccgccgacac ggagccgaag gacggcaccg
     2941 tccacgtcaa agagtggctc gacttcgccg cgcgccgcgt ccccgaaatc cagctcgaag
     3001 agatgtcgcg cgccgccacc agcgggcgcg acctctcctt cgccgaggaa gagcgcggca
     3061 tcaccctcgc ccgccggcag ggtcagcgcc cgcgcctcta ctaccgccgc gaactcgaag
     3121 cccgccccct cgtcgtcgcc aagacccgca actgaaagcg gccctcgcta ctacatactt
     3181 cttgacacgc ccccccgcgt gccatacttt cccttcccac acgggacgcg acacacaatc
     3241 ctttcgaggt gaacacgatg aacgacggaa cctgctggaa ctgcggccac ccccacgcgc
     3301 accacaccat cctcggcggc tgctcctacg aacgcgaagg cgtccgctgc gactgccccc
     3361 gctacgaaga cagcatctac cacggcgaac aacgcaaacg cgacacctac accctctccg
     3421 accgccgccg gttgggcctc ggaagaggta gacggtaagc ccacaagacg ctgagtgaac
     3481 tagccgcaaa ggaggagata tcaagaaagg cttgaagcaa tgaccggcga agagatggag
     3541 cgcgcaatag agttcctgtt gcagaaccat gccacgcacg aggagcggct taggcagacg
     3601 cagcagcaga ttgcggagac gaacaggatt atgcagatgc acgccgagac gcagacggag
     3661 ttcatcaaga tcgtcacgaa ggccatcggc gatttggccg aggcgctgca gcggactgcc
     3721 gaggcgcaga gacggactga cgatagagtt gaccggctgg cggcgacggt cgagcggttc
     3781 atcagcgagg ggcgcgacgg gaaagtgtga ggcgggcgcc gaagggtcgt gcgccttcag
     3841 tctcgcgcgc ctgttgtgtt ttcgcgtcgc gcttcgccgc cctcttgctt ttcgtcttcg
     3901 tcttcgtcgg cggggttgta gatttcgtag ccggtgcggt cgtcgtaata gtagccgccg
     3961 cggggttggc ggtcgcgggg cggcgcgggt gcgggtggtc tgttatcatc gaggggtcgg
     4021 tctcccatct ccatctccgt tcgcgtgact tcgaaggtcg ccttgccgtg cgcggaaaat
     4081 cccagcatca aagcggaatg attatagcgc cgccgcgcgt ctcgcgccgc agggcgggcg
     4141 ggcaaagaaa catgagaatc atggtgacga aaatgtgtgt gagggcggcg gcggcgtgcg
     4201 tcgtcgtgtt ggcgttgctg tcgggcgggt cggtcgcggt cgcgcggcgg cagtcgccgc
     4261 aggcgcgtgc gcagaggttc gagctgacgg tggacagcat catgcgcggg cctgagctgg
     4321 tggggcatcc gccgtcgggc gtggtgtggt cgcaggacag cgggcgcgtg ttcttccgtt
     4381 ggaagcgggc gggcgagccg cgtctgaagg cgatggactg gtacgtggtg aatcgcgacg
     4441 ggacggggct gcggaagctg tcggaggaag aggcgcggcg cgcggggccg cctccggcgt
     4501 cgggcgagtt gtcgaaggac aagacgctga cggtgttcac ggaagacggc gacgtgttcc
     4561 tctacgacca cgcgaagggc gagcggcggc aggtgacgcg gacggtggac gccgagacca
     4621 acgcgcactt cacgcgcgac cagaagcgcg tctacttcac gcggcagaac aacctgtacg
     4681 tgatgtcgct cgacggcg
//
LOCUS       DBNGOH010000803         2674 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3970915, whole genome shotgun sequence.
ACCESSION   DBNGOH010000803 DBNGOH010000000
VERSION     DBNGOH010000803.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2674)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2674)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2674
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3970915"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..283)
                     /locus_tag="AC159P_15430"
     CDS             complement(<1..283)
                     /locus_tag="AC159P_15430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019119300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate decarboxylase"
                     /protein_id="HIR3696893.1"
                     /translation="MHRNLRSFLELLEREKDLAYIDAEVDPYLELAEVHRRVIAEGGP
                     ALLFRRVKGSRYPVVTNLFGTERRIALAFGSKPESLVRQAVHVAVSLMPP"
     gene            complement(413..1015)
                     /locus_tag="AC159P_15435"
     CDS             complement(413..1015)
                     /locus_tag="AC159P_15435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696894.1"
                     /translation="MKSRVTFLLAVAALASVFAGPLAAATAQNDSSALAKRLSSRLPA
                     QRREAAEELARAAAPEHRRLLEGYRVQESDARVRVAMDWALYRMGKGEALFAVVRALD
                     SKQVEQTVAYLKQIEGPDPLYIFLNRVNGNTRIRLLEVLAQTGNAGTLAEIKPFTESL
                     DPAIADAAKFAEREITIRLEETPAAEPKRPRRTGATESEP"
     gene            complement(1123..2628)
                     /locus_tag="AC159P_15440"
     CDS             complement(1123..2628)
                     /locus_tag="AC159P_15440"
                     /inference="COORDINATES: protein motif:HMM:NF017044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HIR3696895.1"
                     /translation="MTSTLNTTRRAALALLLVAASAFAARAQQSTPAPQETPPPPAAP
                     RSVTVPKPVERTLSNGLRVVVIEDHDMPLVSAQLIVKSGGEVDPAALSGVADMTASLL
                     TKGTKARTAPQIASEIESLGGTLTSAAGWDASRATLNVMSSKIEPAMAVLSDVVRNPV
                     FDAEEIERLRQQYADGLSVAMQDPGQLAGYVASRVVFGDAAYGHPVSGTPESIPAIKR
                     EDIVKIHSTYYRPDNSLLVVGGDIAAADAFKLAERLFGDWVKPAAALPAATMAKRDAG
                     AKPRIVVVDMPEAGQAAVVLARRGLSRTDPEYFRGIVSNAVLSGYSGRLNQEIRIKRG
                     LSYGARSSLEARRDTGPFIASAQTKNPSGAEVAGLLVGEIGRLANEAIVETELNPRKS
                     VLIGNFGRSLETTDGLVGQIASLALYGLSLGEINNYIGSVQAVTPAEIQKFASARLGA
                     KDANIVIVGSAKDFVEALRKQFPDVEVIPFAELDLNNATLRKTPAQKSSAN"
BASE COUNT          398 a          844 c          957 g          475 t
ORIGIN      
        1 gcggcggcat gagcgatacg gcgacgtgca cggcttggcg cacgagtgat tcgggtttgc
       61 tgccgaaggc cagcgcgatg cggcgctcgg tgccgaagag gttggtgacg acagggtagc
      121 ggctgccctt cactctgcgg aagaggagcg cggggccgcc ctccgcgatg acgcgccggt
      181 ggacttcggc gagttcgagg taagggtcaa cctccgcgtc gatgtaagcg agatctttct
      241 cgcgttcgag cagttcgaga aaagagcgaa ggtttcggtg catcggaaga taataggggg
      301 aaagggggaa aaggttaaaa ggggaaaagg cgaagaggga ggaaaagcaa gtcgcttctt
      361 ctcccttttc cccttttccc cttttaacct tttccccctt cttgtctgcc ggtcacggct
      421 cggactccgt ggcgccggtg cggcgcgggc gcttcggctc ggcggcgggg gtctcttcga
      481 ggcggatggt gatttcgcgc tcggcgaact tggcggcgtc ggcgatggcg gggtcgagcg
      541 attcggtgaa gggtttgatc tcggcgagcg tcccggcgtt gccggtctgc gcgagcactt
      601 cgagcaggcg gatgcgcgtg ttgccgttga cgcggttgag gaagatgtag agcgggtcgg
      661 ggccttcgat ctgcttgagg taggcgaccg tctgctcgac ctgcttcgag tcgagcgcgc
      721 ggacgaccgc gaacagcgcc tcgcccttgc ccatccggta gagcgcccag tccatcgcca
      781 cgcgcacgcg cgcgtccgac tcctgcaccc ggtagccttc gagcagccgg cggtgttccg
      841 gcgccgccgc gcgcgcaagc tcctccgccg cctcgcgccg ctgtgcaggc agacggctcg
      901 acagccgctt cgccagcgcc gaggaatcgt tctgcgcggt cgccgccgcg agcggcccgg
      961 cgaaaacaga cgcgagggcg gcgacggcga gcagaaaagt aactcttgat ttcaacacgc
     1021 gccttcctcc cgaaataccg tgtcgggtaa accgtcaacc gtgacgagtc ataacaggtc
     1081 ttcacccgtc acggtttacg gttcacgatt cacggctttt attcagttcg cgctcgactt
     1141 ctgcgcgggc gtcttacgca gggtcgcgtt gttgaggtcg agttcggcga agggaatgac
     1201 ctccacgtct gggaactgtt tgcgcagcgc ctcgacgaag tccttcgcgc tgccgacgat
     1261 gacgatgttc gcgtccttcg cgccgaggcg ggcggaggcg aacttctgaa tctcggcggg
     1321 cgtgacggcc tgcacgctgc cgatatagtt gttgatctcg ccgaggctca gcccgtagag
     1381 cgcgagcgag gcgatctgcc cgaccagccc gtcggtcgtc tccagtgagc gaccgaagtt
     1441 gccgatgagg acggacttgc gcgggttcaa ctcggtctcg acgatggctt cgttggcgag
     1501 cctgccgatc tcgccgacga gcagccccgc gacctccgcg cccgaagggt ttttcgtctg
     1561 cgccgaggcg atgaacgggc cggtgtcgcg gcgcgcttcg agcgagctgc gcgcgccgta
     1621 gctcagcccg cgcttgatgc gtatctcctg attgaggcgg cccgaatagc cggagagcac
     1681 cgcgttcgag actatcccgc ggaagtattc ggggtccgtg cgactcagcc ctcggcgcgc
     1741 gagcacgacc gccgcctgcc ctgcctcggg catgtcaacg acgacgatgc gcggctttgc
     1801 gcccgcgtcc cgtttcgcca tcgtcgccgc gggcagcgcc gccgccggct tgacccagtc
     1861 gccgaacagg cgctcggcga gcttgaaggc atcagccgcc gcgatgtcgc cgccgacgac
     1921 gagtaacgag ttgtcggggc ggtaataggt cgaatgaatc ttgacgatgt cctcgcgctt
     1981 gatcgcgggg atggattcgg gcgtgccgga gacgggatgg ccgtaggccg cgtcgccgaa
     2041 gacgacgcgc gaggcgacgt agccggcgag ctggccgggg tcttgcatcg caacgctcag
     2101 gccgtcggcg tattgctggc gcaggcgctc gatctcctcg gcgtcgaaca cggggttgcg
     2161 caccacgtcc gagaggacgg ccatcgccgg ctcgatcttc gaggacatca cgttgagcgt
     2221 ggcgcgcgag gcgtcccaac cggcggcgga ggtgagcgtg ccgccgagcg attcgatctc
     2281 cgaggcgatc tgcggcgcgg tgcgcgcctt cgtccccttc gtcaggagcg aggccgtcat
     2341 gtcggccacg ccgctcaggg cggcggggtc aacctcgccg ccgctcttga cgatgagctg
     2401 cgcggagacg agcggcatgt cgtggtcttc gatgacgacc acgcgcaggc cgttcgagag
     2461 cgtgcgctcg accggcttcg gcaccgtcac cgagcgcggc gcggcgggcg gcggcggcgt
     2521 ctcctgcggc gcgggcgtac tttgttgcgc gcgcgcggcg aaggccgacg cggcgacgag
     2581 cagcagcgcg agtgcggcgc ggcgggtggt gttcagcgtg cttgtcatcg gccctccccc
     2641 ttcttcgcgt cggcgtcgct ggcctgcggc ttcg
//
LOCUS       DBNGOH010000804         2171 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2135876, whole genome shotgun sequence.
ACCESSION   DBNGOH010000804 DBNGOH010000000
VERSION     DBNGOH010000804.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2171)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2171)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2171
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2135876"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..291)
                     /locus_tag="AC159P_15445"
     CDS             complement(<1..291)
                     /locus_tag="AC159P_15445"
                     /inference="COORDINATES: protein motif:HMM:NF012530.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696896.1"
                     /translation="MSVKLTTSLSQRLVLTPQLRQRIEMLQMTSLELSDLIQQQLLEN
                     PILEEVHTQEEKRELEKEILDQLAGAPGDGEPTAFDAAAPAPESANGAGLSPN"
     gene            complement(419..1213)
                     /gene="lptB"
                     /locus_tag="AC159P_15450"
     CDS             complement(419..1213)
                     /gene="lptB"
                     /locus_tag="AC159P_15450"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006933772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HIR3696897.1"
                     /translation="MQTVETNREVVLEATRAGASKSPSAEALAAFELVKHYGRRRVVD
                     GVSLHVEPGEVVGLLGANGAGKTTTFYMIIGLERPDAGRVRLAGRDITKLPMYLRARL
                     GVGYLPQEPSVFRKMTAAENVLAVLETMGLRSRERYKRLEELLEEFGIQHVRNTRGDA
                     LSGGERRRTEIARALATEPQFILLDEPFAGIDPKAVDDIQNMILYLRGRGIGILITDH
                     NVRETLGVTDRAYIMADGRIFRAGAPRELTEDAEVRRLYLGEKFRM"
     gene            complement(1327..>2171)
                     /locus_tag="AC159P_15455"
     CDS             complement(1327..>2171)
                     /locus_tag="AC159P_15455"
                     /inference="COORDINATES: protein motif:HMM:NF015901.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0001530 - lipopolysaccharide binding
                     [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LptA/OstA family protein"
                     /protein_id="HIR3696898.1"
                     /translation="NGQAATMAYTAGDATVRLRGGEPVVWDSRARLKAPEIDSDTRNK
                     VSHARGRVTTTYYSQEQTNGAAPFGKVKSPVFVAAQTAEFQHESGIGIYNGSARMWQD
                     DNFVKADRITLRREQKRMEGDGNVQSALYNAKRKEAGGAQTIVPVNANSNRMSYTETG
                     RLLRYEGNVDIRQGTERITSATADVHLLKDTYEVERTIAERNVVVTQPGKRGTGDWAQ
                     YTAADETVMLTGNPARVEDAEQGASESRRMTILLRENRVVSDGGEGRQNTGRVRTTHK
                     IRKQ"
BASE COUNT          291 a          748 c          695 g          437 t
ORIGIN      
        1 gttcggcgac agacccgcgc cgttcgcgga ctcgggcgcg ggggcggcgg cgtcgaacgc
       61 tgtcggctcc ccgtcgccgg gcgcgcccgc gagctggtcg agaatctcct tctccagctc
      121 gcgcttctcc tcctgcgtgt gtacctcttc gaggatcggg ttttcgagga gctgctgctg
      181 gatcagatcg ctcagttcga gcgaggtcat ctggagcatc tcgatgcgct ggcgcaactg
      241 cggcgtcagg acgagtcgct gcgaaagact ggtggtgagc ttaaccgaca tttacgcttc
      301 ccgctctaac gaaccgggcc ggacgatttc tctttttatg attgctgttg atgccgcgcc
      361 ctcggctgcg gctgcgggag agattataac cgatttgccg ccgccgtgca gcagccactt
      421 acatgcggaa tttttccccg aggtagagcc gccgcacttc cgcgtcctcg gtcagctcgc
      481 gcggcgcccc cgcgcggaag attctcccgt cggccatgat gtaggcgcgg tcggtcacgc
      541 ccagcgtctc gcggacgttg tgatccgtaa tcaggatgcc gatgccgcgc ccccgcaaat
      601 aaaggatcat gttctggatg tcgtccacgg ccttcgggtc gatgccggcg aagggttcgt
      661 ccaagaggat gaactgcggc tcggtggcca gcgcccgcgc gatctctgtg cgccgccgct
      721 cgccgcccga gagcgcgtcg ccgcgcgtgt tgcgcacgtg ctggatgccg aactcttcga
      781 gcagctcttc gagccgcttg tatctttcgc ggctccggag gcccatcgtc tccagcacgg
      841 ccagcacgtt ctccgccgcc gtcatcttcc ggaacacgga cggctcctgc ggcaggtaac
      901 cgacgccgag gcgggcgcgc agatacatcg gtagtttcgt gatgtcgcgc cccgcgagcc
      961 ggacgcgccc ggcgtccggg cgctcgagcc cgatgatcat gtagaaggtg gtcgtcttgc
     1021 ccgcgccgtt cgccccgagc aagcccacca cctcgcccgg ctcgacgtgc agactcaccc
     1081 cgtccaccac ccgccgcctg ccgtagtgct tcaccagctc gaacgccgcc agcgcctccg
     1141 cggacggcga cttcgacgcg cccgcccgcg tcgcctccaa gaccacttcc ctgttcgtct
     1201 cgaccgtctg cataaaaggc ggtgaatagt aaatgttgaa tggtgttcag gctatggtga
     1261 actttgtcta ctgccacaca gaccgaactg ctcgcaaccc tctattcacc attcaccatt
     1321 taccattcat tgttttctga tcttgtgcgt cgtccgcacg cgcccggtgt tctggcggcc
     1381 ctcgccgccg tcggagacga cgcggttctc gcgcaggagg atggtcatgc ggcggctctc
     1441 ggaggcgccc tgctcggcgt cctcgacgcg ggcggggttg ccggtgagca tgacggtctc
     1501 gtccgccgcg gtgtactgcg cccagtcgcc ggtgccgcgc ttgccgggct gcgtgacgac
     1561 gacgttgcgc tcggcgatgg tgcgctcgac ctcgtacgtg tccttcagca gatgcacgtc
     1621 cgccgtggcg ctcgtgatgc gctcggtgcc ttgacggata tcgacgttgc cttcgtagcg
     1681 cagcagccgc ccggtctcgg tgtacgacat gcggttcgag ttggcgttga cggggacgat
     1741 cgtctgcgcg ccgcccgcct ctttgcgctt cgcgttgtag agcgcgctct ggacgttgcc
     1801 atcgccctcc atccgcttct gctcgcggcg cagggtgatg cggtcggcct tgacgaagtt
     1861 gtcgtcctgc cacatgcgcg cgctgccgtt atagataccg atgccggact cgtgctggaa
     1921 ctcggcggtc tgcgcggcga cgaagaccgg actcttgacc ttgccgaacg gcgcggcccc
     1981 gttcgtctgc tcctgactgt agtaggtggt cgtcacccgc ccgcgcgcgt gcgagacctt
     2041 gttgcgcgtg tccgagtcga tctcgggcgc cttgagccgc gcgcgcgagt cccacacgac
     2101 cggctcgccg ccccgcaggc gcacggtcgc gtcgccggcg gtgtaggcca tcgtcgccgc
     2161 ctggccgttg c
//
LOCUS       DBNGOH010000805         8707 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1771635, whole genome shotgun sequence.
ACCESSION   DBNGOH010000805 DBNGOH010000000
VERSION     DBNGOH010000805.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8707)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8707)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8707
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1771635"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..855
                     /locus_tag="AC159P_15460"
     CDS             <1..855
                     /locus_tag="AC159P_15460"
                     /inference="COORDINATES: protein motif:HMM:NF018004.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HIR3696899.1"
                     /translation="DKENDRTRHILTLRAQTGPVFLTYRADRRIDALVEGETHGPPLY
                     DFEAEDGVRHTVWRVGSSEQLVRCFKEVPLLYIADGHHRAAGASRARATLRDQNPSHT
                     GDEGYNRFLAVIFPADQMRILPYNRVVKDLNGLSEDDFLSKVSERFDVTPDANPRGPG
                     KPGHWHMYTGGKWYGLTLEDGGAPKNDEDPTASLDVSVLQDNLLGPVLGIKDVRTDKR
                     IDFVGGIRGTEELVRLVDEGAAAVAFALYPTSIEDLIRVSDAEGIMPPKSTWFEPKLR
                     DGILIHTI"
     gene            967..1503
                     /locus_tag="AC159P_15465"
     CDS             967..1503
                     /locus_tag="AC159P_15465"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3696900.1"
                     /translation="MEIETERLLLRMFTPEDAGALYRIVSDDEFRRYVPRYSPTPEGV
                     AAGIEHMFAHWRERGYGRWALSPKNSRELLGYCGLRLLPETEEVEILYGIDRPHWGRG
                     LVTEAAKATLRFGFEEAKLERVIALAHPANKGTRRVMEKAGLRYEKQAVYFDMECAYY
                     ALDRGRYRADGSLYILRS"
     gene            1529..2737
                     /locus_tag="AC159P_15470"
     CDS             1529..2737
                     /locus_tag="AC159P_15470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011422012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3696901.1"
                     /translation="MRVLIADKFEQSGRDGLEAAGCDYSFQPDVKDETLVEAIRSYQP
                     EALVVRGTKVTEPMMDAGPLKLIVRAGAGYNTIDVAAASRRGVYVSNCPGKNAVAVAE
                     LALALILALDRRVADNVIALREGRWDKKEFSKARGLSGRTLGLIGTGQIGQEVITRAR
                     AFGMRVVAWSRSLSDERAVVLGVERLDSPTDVAREADVVSVHLALNPETRGLIGADFF
                     DAMKEGAYFVNTARGEVVDQQALVRAMREKNIRAGLDVFAAEPTSATGEFTDEVAREP
                     NLYGTHHVGASTDQAQEAIAAETVRIIRTFKETGKVPNVVNLARRTPATHALTVRHRD
                     RPGVLASVLDAVRAADINVQEMENIIFEGSEAAVARINLEAAPPAETLERLKAENADI
                     IELALLEITQ"
     gene            2771..3028
                     /locus_tag="AC159P_15475"
     CDS             2771..3028
                     /locus_tag="AC159P_15475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018467414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696902.1"
                     /translation="MMDVKQAAKLASDYFNSLYETQSLSNVLLEEVELTENGEYWLIT
                     LSYPAQPETVSSIFGTKRQYKVFKINAETGEVQSMKIRKID"
     gene            3031..3531
                     /locus_tag="AC159P_15480"
     CDS             3031..3531
                     /locus_tag="AC159P_15480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015204724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HIR3696903.1"
                     /translation="MNYLDDLLSSYRNKGVLVDTNLLLVYFVGLYDPDRIKRFNRTAA
                     FTVEDFELLCQLLAFFNKVVTTPNILTEVNSLSNKLPDDLKPTFYPVFAQRLAVLEEH
                     YVESKTASSHEYFDRFGLTDSGIISLVQGNYLVLTDDFKLAGYLQKINVDVINFNHIR
                     SINWNI"
     gene            3553..4344
                     /locus_tag="AC159P_15485"
     CDS             3553..4344
                     /locus_tag="AC159P_15485"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017659882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3696904.1"
                     /translation="MKTALITGASGGIGEVFAQKLAARGENVFLVARSGDKLAVICNA
                     LIAEHKVDAQFFALDLTEGDAPARLYEETVRRGLEVETLVNNAGYGSMGEFALLDAGN
                     ELRMIDLNVRALAELTHRYLGPMRERGRGAIVNVASTVSFQPVPFMATYAATKAFVLS
                     FSEALWEENRAHGVKVLALCPGSTDTNFFDAAGSAPPPRSVVQTPEEVVEAAFGALDA
                     GKGHVVSGWKNFLLAESNRLAPRSLSTRIAGMILKQRYKPKSREP"
     gene            4467..5552
                     /gene="serC"
                     /locus_tag="AC159P_15490"
     CDS             4467..5552
                     /gene="serC"
                     /locus_tag="AC159P_15490"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HIR3696905.1"
                     /translation="MTERIFNFSAGPAVLPVPVLEQARDELLSLPGVGMSVMEISHRS
                     KTFDEIIGGAEANLRELMGIPENYRVLFLQGGASLQFSMIPLNLLGEGRSADYIITGS
                     WGKKALKEAKKQGATSVAADMADSRYSRVPDDGELRLDPAAAYVHMTSNETIEGVEFK
                     GEPDTGSVPLVNDASSNICSRPIDVGKYALIYAGAQKNLGPSGLTLVILRDDLLQRIP
                     AGLHTMLDYRTHVENQSLYNTPNTWGIYILNLVGRWIKEQGGLAGIARVNEEKARLIY
                     DRIDATDFYRGHAATEARSNMNVTFRLPTEELEKKFTAEATAQGLDGLKGHRSVGGIR
                     ASIYNAFPRAGVEALVSFMKEFEAKNG"
     gene            5770..6864
                     /locus_tag="AC159P_15495"
     CDS             5770..6864
                     /locus_tag="AC159P_15495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolB family protein"
                     /protein_id="HIR3696906.1"
                     /translation="MKTKVLFPLVAACTALAAAFSFAQDKPGADPRPAHGGGGSSVVE
                     RADEKHLRNVRQLTNGGENAEAYFSGDGRRLIFQSKRDGRRCDQIYTMLTDGSDLRMV
                     STGGGTTTCSYIFPSGKRLLFSSTHLAGAECPPPPDRSRGYVWAIYPSFDIFTARADG
                     KDLKRLTHAPGYDAEATMSADGKKIVFTSTRDGDLDIYTMDADGRNVRRLTNELGYDG
                     GPFFSRDGKKIVYRAHHPKTPEQVERYKKLLAENVIEPNTFELWVMDADGKNKKQVTN
                     LGVASFAPYFTPDGRRIIFASNYPSRRSRNFDLYMVNLDGTGLERVTYNETFDGFPMF
                     SPDGKKLVFASNRNAAREGDTNVFIADWVE"
     gene            7010..>8707
                     /locus_tag="AC159P_15500"
     CDS             7010..>8707
                     /locus_tag="AC159P_15500"
                     /inference="COORDINATES: protein motif:HMM:NF016289.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HIR3696907.1"
                     /translation="MLYKKHLRTPLLAVLLSSVALAQQPVAIKPAAAVELERLRAHVT
                     HLASPKLEGRKTGTRGADEAADYIAAEFKKLSLAPGGNTGTGPRNEVVTREYLQRFPY
                     VAGVELGKDNELEFTVRDKSLPPVAIDFRVGEDWMPLGFSANARVHEAPVMLVGYGIT
                     ATELNHDDYAGGRAAGHIAIALSGTPDGGNPHGGFARAGEVRFKAAAARAAGAKALVI
                     VASEDDFKENKLSRLSYDNAGGDAGFPVAVISRQAVVRMLRASGASPSVVALWEKGFR
                     EQKPSPQSGGSHQIHVIQEGPVPRLTISTDIVRKEAPAANVVGVLKGSDEKLEDEVIV
                     VGAHYDHLGHGGRGSLAGREGGIHHGADDNASGTAALIELARVLSAERARARRTVVFI
                     AFGGEEEGLLGSSYYVNHPAAPLEKTVAMINLDMVGRLRGDALMIGGVGTAAEWRAMI
                     EEANNALEVKVVAASHGGGVGSPASDDKPSMPIVTGADGRVVATASTTRRFNLRLNED
                     GFGPSDHSSFYAKRIPVLFFFTGTHEDYHKPTDTADRINYDGLAAVAEFVRRILSDLQ
                     "
BASE COUNT         1694 a         2835 c         2739 g         1439 t
ORIGIN      
        1 gacaaggaga acgaccgcac gcgccacatc ctgaccctgc gcgcgcagac cggccccgtc
       61 ttcctcacct accgcgccga ccgccgcatc gacgcgctcg tcgaaggcga gacgcacggg
      121 ccgccgctct acgacttcga agccgaggac ggcgtgcgcc acaccgtctg gcgcgtcggc
      181 agttccgaac aactcgtccg ctgcttcaag gaggtgccgc tcctctacat cgccgacggc
      241 caccaccgcg ccgccggcgc cagccgcgcc cgcgcgacct tgcgcgacca gaacccgagc
      301 cacaccggcg acgaagggta caaccgcttc ctcgccgtca tcttccccgc agaccagatg
      361 cggatactcc cctacaaccg cgtcgtcaaa gacctgaacg gactctcgga agacgacttc
      421 ttatcgaagg tctcggaacg tttcgacgtg acgccggacg ccaacccgcg cggccccggc
      481 aagcccggcc actggcacat gtacacgggc ggcaagtggt acggcctgac gctcgaagac
      541 ggcggcgctc cgaagaacga cgaagacccg accgcctcgc tcgacgtgag cgtcttgcaa
      601 gacaacctgc tcggccccgt gctcggcatc aaggacgtgc gcacggacaa gcgcatcgat
      661 tttgtcggcg gcatccgcgg caccgaagaa ctcgtgcgcc tcgtggacga gggcgcggcg
      721 gcggtcgcct tcgcgctcta cccgacttcc atcgaagacc tcatccgggt ctccgacgcc
      781 gaaggcatca tgccgcccaa gtccacctgg ttcgagccga agctgcgcga cggcatattg
      841 attcatacga tatagcagtg gcggtaggcg gtaggcagtc aaagcaatcg aagaggttga
      901 agttccaaaa ttcgaattcg aaagccgacg gttcttaact gcctactgcc cgctgcctac
      961 tgccttatgg aaatcgagac cgagcgcctg ctgctcagga tgttcacgcc ggaggacgcg
     1021 ggcgcgctct accgcatcgt gagcgacgac gagttccgca ggtacgtgcc gcggtattcg
     1081 ccgacgccgg aaggggtcgc ggcgggcatc gaacacatgt tcgcgcactg gcgcgagcgc
     1141 ggttacggac ggtgggcctt gtcgcccaaa aacagccgcg aactgctcgg ctactgcggc
     1201 ctgcgcctgc tccccgaaac ggaagaggtg gaaatcctct acggcatcga caggccgcac
     1261 tggggccgcg ggctggtgac cgaggcggcc aaagcgacgc tgcgtttcgg gttcgaagag
     1321 gcgaagctgg aacgcgtcat cgcgctcgcg caccccgcca acaaagggac gcgccgcgtc
     1381 atggagaagg cgggcctgcg ctacgagaag caggccgtct acttcgacat ggagtgcgcc
     1441 tactacgcgc tcgaccgcgg gcgttaccgc gcggacggct cgctctacat cctgcgttcg
     1501 tgagtaattt cggagaggct tgaaaatcat gcgcgtgttg attgccgata aattcgaaca
     1561 gtcgggccgc gacgggcttg aggccgcggg ctgcgactac tctttccagc cggacgtgaa
     1621 ggacgagacg ctggtcgagg ccatccgctc ttaccagccc gaagcgctcg tcgtgcgcgg
     1681 gacgaaggtg accgagccga tgatggatgc agggccgttg aagctcatcg tgcgcgcggg
     1741 cgcgggctac aacacgatcg acgtggcggc ggcctcgcgc cgcggcgtct acgtctcgaa
     1801 ctgccccggc aagaacgccg tcgccgtcgc cgagttggcg ctggcgctca tcctcgcgct
     1861 cgaccgccgc gtcgccgaca acgtcatcgc gctccgcgaa gggcgctggg acaagaagga
     1921 gttctcgaag gcgcgcggcc tctccggtcg cacgctcggg ctgatcggca cgggccagat
     1981 cgggcaggag gtcatcacgc gcgcgcgcgc cttcgggatg cgcgtcgtcg cttggagccg
     2041 cagcctctcg gacgaaaggg cggtcgtgct cggcgtcgag cgactggaca gccccactga
     2101 tgtggcgcgc gaagcggacg tggtcagcgt ccacctcgcc ctgaaccccg agacgcgcgg
     2161 gctgatcggc gccgacttct tcgacgcgat gaaggagggc gcctacttcg tcaacaccgc
     2221 gcgcggcgaa gtggtcgatc aacaggcgct cgtgcgcgcg atgcgcgaga agaacatccg
     2281 cgccggcctc gacgtgttcg ccgccgagcc gacgagcgcg accggcgagt tcaccgacga
     2341 ggtcgcgcgc gagccgaacc tctacggcac gcaccacgtc ggcgcctcga ccgaccaggc
     2401 gcaggaggcc atcgccgccg agaccgtccg catcatccgc accttcaaag agacgggcaa
     2461 ggtgcccaac gtcgtcaacc tcgcgcggcg cacgcccgcg acgcacgcgc tcaccgtccg
     2521 ccaccgcgac cggcccggcg tcttggcctc cgtcctcgac gccgtgcgag ccgccgacat
     2581 caacgtgcag gagatggaga acatcatctt cgaaggctcc gaggccgcgg tcgcccgcat
     2641 caacctcgaa gccgcccctc ccgccgaaac gctcgaacgc ctgaaggccg agaacgcgga
     2701 catcatcgaa ctggccttac tggaaatcac acaatagaat actaatcaaa gctcaggagg
     2761 atagggacgt atgatggatg tcaagcaagc agccaagttg gcctctgatt attttaacag
     2821 cctgtacgag actcagtctt tgtcgaatgt cttgctggaa gaagttgaat tgacagagaa
     2881 cggggaatac tggctgatta ctcttagtta tccggcgcag ccggaaactg tgtctagcat
     2941 cttcggcact aagaggcaat ataaggtctt taaaattaat gcagaaacgg gggaggtgca
     3001 gtcaatgaaa ataaggaaaa tcgattaggc atgaactatc tcgacgacct gttatctagt
     3061 tatcgtaata aaggagtatt ggttgatacc aacctattgc tggtttattt cgtaggctta
     3121 tatgaccctg atcgaattaa gaggttcaac agaacggcgg cttttactgt tgaagatttc
     3181 gaattgctat gccaacttct ggccttcttt aataaagttg tgactactcc gaacatcctc
     3241 accgaagtaa acagcttatc gaacaagttg cctgacgatt taaagcctac attctatcct
     3301 gtattcgcac aacgcctcgc cgttttggaa gaacattacg tcgagagcaa aacggcctcc
     3361 tcgcacgaat atttcgacag gtttggtctc acggattcgg ggattattag tttggttcaa
     3421 ggaaactacc ttgtcttgac ggatgacttc aaactggcgg gctatttgca aaagattaat
     3481 gttgatgtca ttaactttaa ccatatcagg tctattaact ggaacattta gcgccgataa
     3541 ggttcctgtc tcatgaagac tgcactcatc acgggcgcgt cgggcggcat cggcgaggtg
     3601 ttcgcgcaaa agctcgcggc gcggggcgag aacgtgtttc tggtggcgcg gtcgggggac
     3661 aagctggcgg tgatctgcaa cgcgctgatc gcggagcaca aggttgacgc gcagttcttc
     3721 gcgctcgatt tgacggaagg agacgcgccc gcgcggctgt atgaagagac ggtgcggcgc
     3781 ggcctcgaag tggagacgct ggtcaacaac gcgggctacg ggtcgatggg cgagttcgcg
     3841 ctgctcgacg cggggaacga gctgcggatg atcgacctca acgtgcgtgc gctcgcggag
     3901 ctgacgcacc gctacctcgg gccgatgcgc gagagggggc gcggcgcgat cgtcaacgtc
     3961 gcctcgacgg tctcgttcca gcccgtgccc ttcatggcca cttacgcggc gacgaaggcg
     4021 ttcgtgctct ccttctccga ggcgctgtgg gaggagaacc gcgcgcacgg cgtgaaggtg
     4081 ctggcgctct gccccggctc gaccgacacg aacttcttcg acgccgcggg cagcgcgccg
     4141 ccgccgcgct ccgtcgtcca gacgcccgaa gaggtcgtcg aggcggcctt cggcgcgctc
     4201 gacgcgggca agggccacgt cgtctcgggc tggaaaaatt tcctgctcgc agagtccaac
     4261 cgcctcgccc cgcgctcgct ctcgacgcgc atcgccggga tgattttgaa gcagcggtac
     4321 aaacccaaga gccgtgaacc gtaaaccgta aaccgtgatg agcaagaatg gaattcgact
     4381 tatcactcgt cactcgtcac ctttcattgc tttcatccgt tcacggttca cggtttacgg
     4441 ttcacggcct ttttcaggag agaacaatga ccgagagaat cttcaacttc agcgcggggc
     4501 cggcggtgct gccggtgcct gtgctcgaac aggcgcggga cgagctgcta agcttgccgg
     4561 gcgtggggat gagcgtgatg gagatcagcc accgctcgaa gacgttcgac gagatcatcg
     4621 gcggcgccga ggcgaatttg cgcgagctga tgggcatccc cgagaactac cgcgtcctct
     4681 tcctgcaggg cggcgcgagc ctacagttct cgatgatccc tttgaacctc ttgggcgaag
     4741 gccggagcgc cgactacatc atcacgggga gctggggcaa gaaggcgctc aaggaggcga
     4801 agaaacaggg cgccacctcg gtcgccgcgg acatggccga cagccgctac tcgcgcgtgc
     4861 ccgacgacgg cgagctgagg ctcgaccccg cggcggctta cgtccacatg acttcgaacg
     4921 agacgatcga gggcgtcgag ttcaagggcg agccggacac gggcagcgtg ccgctcgtca
     4981 acgacgcctc ctcgaacatc tgctcgcgcc cgattgatgt cggcaagtac gcgctcatct
     5041 acgcgggcgc gcagaagaac ctcggcccgt cgggcctgac gctcgtcatc ctccgcgacg
     5101 acctcttgca gcgcatcccg gcggggctgc acacgatgct cgactaccgc acgcacgtcg
     5161 agaaccagtc gctctacaac acgccgaaca cgtggggcat ctacatcctc aacctcgtag
     5221 gccgctggat caaagagcag ggcggcctcg caggcatcgc gcgcgtgaac gaggagaagg
     5281 cgcggctcat ctacgaccgg atcgacgcca cggacttcta ccgcggccac gccgcgacgg
     5341 aggcgcgctc gaacatgaac gtcacgttcc gcctgcccac ggaggagctg gagaagaagt
     5401 tcaccgccga ggcgacggcg caggggcttg acggactgaa gggccaccgc tcggtcggcg
     5461 gcatccgcgc ctcgatctac aacgccttcc cgcgcgcggg cgtcgaggcg ctcgtctcct
     5521 tcatgaagga gttcgaggcg aagaacggat gaggggattg cggattgcgg attgcggatt
     5581 gcggattgcg gattagcaga agcgttgacg attgatgaca gcgccgctcc catcaatcat
     5641 ccgtcttcaa tccgcaatcc gcattcgccc cgccgtttgc gccgaccgtg cgtgcggttt
     5701 aaagtagtgg cgtctccgct ctgcttcatc tccggtgcgt gttcacttga ataaacgtct
     5761 gaacctttca tgaaaacaaa agtgctgttc cccctcgtcg ccgcctgcac ggcgctggcc
     5821 gcggcgttct ccttcgcgca ggacaaaccc ggcgccgacc ctcggcccgc ccacggcggc
     5881 ggcggcagct cagtcgtcga gcgcgccgac gagaagcacc tgcggaacgt gcggcagttg
     5941 accaacggcg gcgagaacgc ggaagcctac ttcagcggcg acggccggcg gctcatcttc
     6001 cagtcgaagc gcgacgggcg ccggtgcgac cagatttaca cgatgcttac ggacggctcc
     6061 gacctccgca tggtctcgac cggcggcggc accaccacct gctcctacat cttcccctcg
     6121 ggcaagcgcc tgctcttctc ctccacccat ctcgcgggcg cggagtgccc gccgcccccc
     6181 gaccgctcgc gcggctacgt gtgggcgatt tacccttcgt tcgacatctt cacggcgcgc
     6241 gccgacggca aggacttgaa gcgcttgaca catgcgcccg gctacgatgc cgaggccacg
     6301 atgtctgcgg acggcaaaaa aatcgtcttc acctcgacgc gcgacggcga cctcgacatc
     6361 tacacgatgg acgccgacgg caggaacgtc cgccgcctga cgaatgagct gggctacgac
     6421 ggcggcccct tcttctcgcg cgacggcaaa aagatcgttt atcgcgcgca ccacccgaag
     6481 acgcccgagc aggtcgagcg gtataaaaaa ctgctggccg agaacgtcat cgagccgaac
     6541 accttcgagc tgtgggtgat ggacgccgac gggaagaata aaaagcaggt gactaacttg
     6601 ggcgtggcga gcttcgcgcc gtacttcacg cccgacggcc ggcgcatcat cttcgcgtcg
     6661 aactacccga gccgccgcag ccgcaacttc gacctctaca tggtgaacct cgacggcacg
     6721 gggctggagc gcgtcaccta caacgagacc ttcgacggct tcccgatgtt ctcgcccgac
     6781 ggcaagaaac tggtcttcgc ctcgaaccgc aacgccgcca gagagggcga caccaacgtc
     6841 ttcatcgccg actgggtgga gtgaaccatc gccccccgaa ggcgcatcat agccttcgtc
     6901 aaggccgaaa gtgcatcaca acgttcggaa agtccgtcgg tttcgaagcg catccatcct
     6961 tccggaagat tttgacgggc accattgccc gcgaacggat tcgaaagcca tgctgtacaa
     7021 gaaacacctc cgcacgccgc tcctcgccgt cctgctctct tcggtcgcgc tcgcgcagca
     7081 gcccgtcgcc atcaagcccg cggcggcggt cgaactcgaa cgcctgcgcg cgcacgtcac
     7141 gcacctcgct tcgcccaaac tcgaagggcg caagaccggc acgcgcggcg ccgacgaggc
     7201 cgccgactac atcgccgccg agttcaaaaa gctgagcctc gcgccgggcg gcaacacggg
     7261 cacggggccg cgcaacgagg tcgttacgcg cgagtacctg caacggttcc cttacgtcgc
     7321 gggcgtcgaa ctcggcaagg ataacgagct ggagttcacc gtgcgcgaca agtcgttgcc
     7381 gcccgtcgct atcgacttcc gcgtcggcga agactggatg ccgctcggct tcagcgcgaa
     7441 cgcgcgcgtc cacgaagcgc cggtaatgct ggtcggctac ggcatcacgg caacggaatt
     7501 gaaccacgac gactacgcgg gcgggcgtgc cgcggggcac atcgccatcg cgctttcggg
     7561 cacgcccgac ggcggcaacc cgcacggcgg gttcgcgcgg gcgggcgagg ttcgcttcaa
     7621 ggcggcggcg gcgcgcgcgg cgggcgcgaa ggcactcgtc atcgtcgcca gcgaggatga
     7681 cttcaaggag aacaagctct cgcgcctctc gtacgacaac gcgggcggcg acgcagggtt
     7741 tcccgtcgcc gtcatctcaa ggcaggcggt ggtaaggatg ttgagggcga gcggcgccag
     7801 tccgtccgtg gtcgcgttgt gggagaaggg tttccgcgag cagaagcctt cgccgcagag
     7861 cggtggcagt catcagatac acgtgatcca ggaaggcccc gtcccccgtc tcaccatctc
     7921 gaccgacatc gtgaggaaag aggcgcccgc cgcgaacgtc gtcggcgtgc tcaaggggtc
     7981 ggacgagaag ttggaagatg aagtgatcgt cgtcggcgcg cactacgacc acctcgggca
     8041 cggcgggcgc gggagcctcg ccgggcgcga gggcgggatt catcacggcg cggacgacaa
     8101 cgcctcgggc acggcggccc tcatcgagct ggcacgcgtc ctctcggcgg agcgtgcgcg
     8161 cgcgaggcgc acggtggtct tcatcgcctt cggcggcgag gaggagggct tgctcggttc
     8221 gagttattat gtcaaccacc ctgcggcgcc gctcgaaaaa acggtggcga tgatcaacct
     8281 cgacatggtg ggccgcctgc gcggcgacgc gctgatgatc ggcggcgtcg gcaccgccgc
     8341 ggagtggcgc gcgatgatcg aggaggcgaa caacgcgctc gaagtcaaag tcgtcgccgc
     8401 gtcccacggc ggcggcgtgg gttcgcctgc ctcggacgac aaaccctcga tgccgatcgt
     8461 gacgggcgcg gacgggcgcg tcgtcgccac cgcctcgacg acgcggcgct tcaacctgcg
     8521 gctcaacgaa gacggcttcg ggccgagcga ccactcttcg ttctacgcga agagaatccc
     8581 cgtgctcttc ttcttcaccg gcacgcacga ggactaccac aagcccacgg acaccgccga
     8641 ccgcatcaac tacgacggcc tcgccgcggt cgccgagttc gtccgccgca tcctctccga
     8701 cttgcag
//
LOCUS       DBNGOH010000806         7706 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_853250, whole genome shotgun sequence.
ACCESSION   DBNGOH010000806 DBNGOH010000000
VERSION     DBNGOH010000806.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7706)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7706)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7706
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_853250"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..913
                     /locus_tag="AC159P_15505"
     CDS             <1..913
                     /locus_tag="AC159P_15505"
                     /inference="COORDINATES: protein motif:HMM:NF017984.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HIR3696908.1"
                     /translation="LDAESQGWHYSRPWVEAHRQEVELLLAFIRERGPVRAADFARTD
                     GKASGWWEWKTEKRALEMLFTAGELMIARRRNFQRVYDLRERVLPSWDDRQSLRIEEV
                     RRESALKAVQALGVTTSRWVSDYFRTGKRETVETVSALAREGALHTVKVEGWKEPALV
                     HPENRGLLKAAAAGKLGAELTTLLSPFDPLVWDRERARAAFGFDYRLECYTPAAKRRY
                     GYFTLPVLRRGALVGRLDAKAHRKEGVFEVKSIHIEPDVRAGEELMSDVAAAIRECAA
                     WHKAPEVVVRESEPREVADRLMKFIER"
     gene            complement(967..3426)
                     /locus_tag="AC159P_15510"
     CDS             complement(967..3426)
                     /locus_tag="AC159P_15510"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR3696909.1"
                     /translation="MIGRTLLHYRIEKKLGEGGQGTVYKALDTKLGRTVVLKVLPPEL
                     VAHPSGFRRFEREARLASALDHPNICTVFDLNESDGTHFIVMQYVRGKDVRQLVGGKP
                     LELRGALSVAVQVADALAAAHAEGIIHRDIKPSNVMVTEAGQVKVLDFGLAKLIDEAA
                     GAGGIHRSHTTEVGVPHGTAASAAPEQAAGKKVDHRADIFSTGVVLYEMLTGVWPFQG
                     KSVVEVRYAVIHDTPRPVAELRAGVPPRLQQILDRALAKDPEARYQRIEELRDELRAV
                     LRETAADEAATLPTDVTPVPPRRLAGTGGVAGTIRRWLRGLAGGAVSRDASPAASSSS
                     AARGSSQTGAGESSGAGAAVTKKTLAILPFRHLGNDPSVSFYEFSLADAVITELARLR
                     ELVVRPSSLVAKYQGLDTDPREIGRELGVGAVLAANYLTAGARLRVNAQLLDVERGDL
                     LWSDRIDAEAADIIAVQDQIARRIVERLSLELSAGERAELERRPTEDAAAYEEYLKGR
                     DRLARYAYHAPAPADFEAAVEHFRRAVRRDPSFALAHSGLGVCHTTRIFDKLGSEEDY
                     ERAEEAFQKALKLDPSLVEARLHMVFIYLTRGEKRKARTEIVGLLREAPNDAAVHRVA
                     ANLYRLDGDHERALEHFDRAGRLNPEERVNAHYNRARVFNSLGQTDNALLELDKASGI
                     EPDHPLIKTVRAFMLYYRGETEAAAEWVRAVLAARPEMDGVRPLLAMCLSRQGRHEEA
                     CAELTERVKVAASADPDIAYWLASAYALEGMADEAFEWLGRAITLGREDRAWFEADPD
                     WSNLRGDPRFAELMRRLGAER"
     gene            3765..4763
                     /locus_tag="AC159P_15515"
     CDS             3765..4763
                     /locus_tag="AC159P_15515"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020531352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR3696910.1"
                     /translation="MKVLFIGGTGFISTAVSRQAVAKGLELYLLNRGLRGAAPHGAHS
                     LTADIRQPEEVGAAMRGLRFDVVVNWVAYTPDDIERDLSLFEGRVGQYVFISSAAVYQ
                     KPPAHYLITEETPLRNPFWDYARDKIACEERLLRAHVEDGFPATIVRPSHTYETNFPV
                     AVGGGGSYTLADRLKRGLPVIVHGDGTSLWVLTHAEDFGRGFLGLLGNREAIGQAFHI
                     TSDEVLTWERIYETIAEALGVEANVVHIPSDFIARVAPGRAGSLLGDKQWSAVFDNSK
                     IKRFVPEFRAVIPFREGIRRTLEWFEMDERRRRVDEGMREEMERILGAYGARYRSV"
     gene            4990..5616
                     /locus_tag="AC159P_15520"
     CDS             4990..5616
                     /locus_tag="AC159P_15520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696911.1"
                     /translation="MNRMVKLAARGTTVLLLAPVFALSVLGRQSGTPPRSGVDRVLAE
                     RARVTNNRRIEEGLRKPAARGAEPQLALAQIKEDYVQIQVVTGDLARAASQGGALDPK
                     FVAKSASEIRKRAERLKHNLALPEPEQAAERPSAEAGDEEARLRASVSTLGKLIAGFV
                     RNPIFRQVGVVDARSAATARRDLEEIIGLSGRLKKESERLSKAVQKSH"
     gene            5989..>7706
                     /locus_tag="AC159P_15525"
     CDS             5989..>7706
                     /locus_tag="AC159P_15525"
                     /inference="COORDINATES: protein motif:HMM:NF051698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR3696912.1"
                     /translation="MVFLVMASASAVAQDYRGKVQGTVVDVNGAAVVGAGVTLRNDGT
                     GVAVTRQTNDDGRYIFDFVDPGTYTVTVEQTNYKKFEQQNVVVRNRGDVTADATLEAG
                     GVEDVVTVTAPAAEVEFNTSGSALTIENKIVDQLPIRGRNPYNIATLDPTVSPGTGST
                     ANENRPYHHAFANDIDAGGGTRSGNDVQLDGVPLTSSFKTSYTPNMDAVQEVTFQKNA
                     VDSEYGYSSGGIVIVNTKGGTNEFHGTAFVNGRSPRFNAVSDPTVLRIAGSDETLRRG
                     SNLKIYGGSVGGPILRNKLFFFATYEKWTDSQPATFITTVPTEAERRGDFSQSSRGVR
                     NIFDPFTSTGASGVRQQFPGNIIPASRFDPTALRLLDQIPLPTLPGNLNNLEVIKTNK
                     TTYWNFSTRVDWNHSERWKTFVRYGQFKANLLEENPTSAALLPINGSNRYGLSIAADT
                     VYTLSSTMVLNLRGRYNSLTDAYAAEPAQLGPEGFAALFPGTNFSQQILTGPNVYYPG
                     IDMQVGGTTYRLGRPGREFFQEPQGYGGSARLNIYLGDHAVKFGGELRVDKGKGARYE
                     PLNLRFT"
BASE COUNT         1273 a         2568 c         2655 g         1210 t
ORIGIN      
        1 gctcgacgcc gagtcgcagg ggtggcacta ctcgcgcccg tgggtcgagg ctcaccggca
       61 ggaagtggaa ctcctcctcg ccttcatccg cgagcggggg ccggtgcgcg ccgccgactt
      121 cgcgcgcacg gacgggaagg ccagcggctg gtgggagtgg aagacggaga agcgtgcgct
      181 ggagatgctc ttcacggcgg gcgagctgat gatcgcgcgg cggcggaact tccagagggt
      241 ctacgacctg cgcgagcgcg tgctcccctc ctgggacgac cgtcagtctt tacgaataga
      301 agaggtgcgg cgagagtcgg cgctcaaggc cgtccaagct ctgggcgtca ccacttcgcg
      361 gtgggtgagt gactacttcc gcaccggcaa gcgggagacg gtggagacgg tgtcggcgct
      421 cgcgcgcgaa ggagcgctgc acacggtcaa ggtcgaaggc tggaaggagc cggccctcgt
      481 ccatccggag aatcgcgggc tgctgaaggc ggcggctgcg ggcaagctcg gggccgagtt
      541 gaccacgctg ctctcgccgt tcgacccgct ggtgtgggat cgagagcggg cgcgcgcggc
      601 gttcggcttc gactaccggc tggagtgcta cacgcccgcg gcgaagcggc gttacgggta
      661 tttcactctg cccgtcctgc gccgcggcgc gctcgtcggg cggctcgacg cgaaggcgca
      721 ccggaaggag ggcgtcttcg aggtcaaatc catccacatc gagccggacg tgcgtgcggg
      781 cgaggagctg atgagcgacg ttgccgcggc gatacgcgag tgcgccgcct ggcacaaggc
      841 tccggaagta gtagtgcgtg agtccgagcc gcgcgaagtc gccgaccgcc tgatgaagtt
      901 catcgagcga taacagcacg aactcgggca cggatatgtt ggcgcaggac gaagctattc
      961 gtttccttat cgctccgcgc cgagccgccg catcagctcc gcaaagcgcg ggtcgccgcg
     1021 taagttcgac cagtcggggt cggcctcgaa ccacgcgcga tcctcgcggc ccagtgtgat
     1081 cgcccgcccc agccactcga acgcctcgtc cgccatccct tcgagcgcgt aggccgacgc
     1141 cagccagtag gcgatgtccg ggtcggcgga ggccgcgacc ttgacgcgct cggtcagctc
     1201 ggcgcacgcc tcttcgtgtc gcccctgccg gctcaggcac atcgcgagca gcgggcgcac
     1261 gccgtccatc tccgggcgtg cggcgagcac ggcgcgcacc cactccgcgg cggcctcggt
     1321 ctcgccgcgg tagtagagca tgaaggcgcg caccgtcttg atgagcggat ggtccggctc
     1381 gatcccgctt gccttgtcga gttcgagcag cgcattgtcc gtctggccga ggctgttgaa
     1441 gacgcgcgcg cggttgtagt gcgcgttgac gcgctcctcg gggttgagcc ggcccgcgcg
     1501 gtcgaagtgt tcgagcgcgc gctcgtggtc gccgtccaaa cggtagaggt tggccgcgac
     1561 gcggtgcacc gcggcgtcgt tcggcgcctc gcgcagcaga ccgacgatct ccgtgcgcgc
     1621 cttccgcttc tcgccgcgcg tcaagtagat gaagaccatg tggagccgcg cctcgacgag
     1681 tgacgggtcg agcttgagcg ccttctgaaa cgcctcctcg gcgcgctcgt agtcctcttc
     1741 gctgccgagc ttgtcgaaga tgcgcgtggt gtggcagacg ccgaggccgg agtgcgcgag
     1801 cgcgaacgag gggtcgcggc ggacggcgcg gcggaagtgc tcgaccgccg cctcgaagtc
     1861 ggcgggcgcg ggcgcgtggt aggcgtagcg cgcgagccgg tcgcgcccct tcaggtactc
     1921 ttcgtaggcg gcggcgtcct cggtcggtct gcgctcaagc tcggcccgct cgcccgcgct
     1981 cagctcaaga ctcagacgct cgacgatgcg ccgcgcgatc tgatcctgca cggcgatgat
     2041 gtcggcggcc tcggcgtcga tgcggtcgct ccacaagagg tcgccgcgct cgacatcgag
     2101 cagttgcgcg ttgacgcgca ggcgcgcgcc cgccgtcagg tagttggccg ccagcaccgc
     2161 gccgaccccc agctctcgac cgatctcgcg cgggtccgtg tccagcccct gatacttggc
     2221 gacgagcgag gacgggcgca cgaccagctc gcgcagccgc gccagctcgg tgatgaccgc
     2281 gtcggcgagc gagaactcgt agaagctgac cgaagggtcg ttgccgaggt ggcggaaagg
     2341 caggatggcg agagtctttt tcgtgaccgc ggcgcccgcg cccgacgact ctcccgcgcc
     2401 ggtctgcgac gagccgcgcg cggcactgct cgacgacgcg gcgggcgacg cgtcgcgcga
     2461 cacggcgccg cccgcgaggc cgcgcagcca gcggcggatc gtgcctgcca cgccccccgt
     2521 cccggcgaga cggcgcggcg gcacgggcgt cacgtcggtc ggcagcgtcg cggcttcgtc
     2581 cgccgcggtc tcgcgcagga cggcgcgcag ctcgtcgcgc agctcctcga tccgttggta
     2641 acgcgcctcc gggtctttgg cgagcgcgcg gtcgagaatc tgttggaggc gcggcggcac
     2701 gcccgcgcgc agctccgcga cggggcgcgg cgtgtcgtgg atgacggcgt agcgcacctc
     2761 gacgaccgac ttcccctgaa acggccagac gccggtcaac atctcgtaca gcaccactcc
     2821 cgtcgagaag atgtcggcgc ggtgatcgac cttctttccg gcggcctgct cgggcgcggc
     2881 ggaggccgcg gtgccgtgag gcacgccgac ctccgtcgtg tgcgagcggt ggatgccgcc
     2941 cgccccggca gcctcgtcga tcagcttggc cagcccgaag tccaagacct tcacctgccc
     3001 cgcctcggtc accatcacgt tcgagggctt gatgtcgcgg tggatgatcc cttcggcgtg
     3061 cgccgccgcg agcgcgtccg ccacctgcac ggcgaccgag agcgcgccgc gcagctcaag
     3121 gggcttgccg ccgacgagct gccgcacgtc cttgccacgg acgtactgca tgacgatgaa
     3181 gtgcgtgccg tccgactcgt tgaggtcgaa gacggtgcag atgttcgggt ggtcgagcgc
     3241 ggaggcgagc cgcgcttcgc gctcgaagcg gcggaagccg gaggggtggg caacgagctc
     3301 cggcggcagg actttgagga cgaccgtgcg cccgagcttc gtgtcgagcg ccttgtagac
     3361 cgtgccctgc ccgccctcgc cgagcttctt ctcgatgcgg taatggagca gcgtccgtcc
     3421 gatcatcgga tgcctcgcgg gttccgccct gccggcgagc cgtggcttgc ccggcgctca
     3481 cctgagccgg cagatgtcca acacgttcat gtcaaggtag ggccgcgccc gaccccgcgg
     3541 ggtcttacgt cacgagtccc ttcgattcgg agaggccatg aatgcggtca tcatacccta
     3601 atcgtcgggc gacctcacct tccgtcgggc aaagggtttc gtcgggcaaa gccgcgacga
     3661 ggcgaacggc atgttgtcaa gggggcggcg ttgtccctat gttatccccg accaagaagt
     3721 tcgggtgtca gggttcgagt gcgagccgcg caactccagc gatgatgaaa gtcttgttta
     3781 tcggcggcac cggcttcatc agcacggcgg tgagccggca ggcggtcgcc aaaggtctcg
     3841 aactgtatct gctcaaccgc gggttgcggg gcgcggcccc gcacggcgcc cacagcctga
     3901 ccgccgacat ccgtcagccg gaagaggtcg gcgcggccat gcgaggcttg cggttcgatg
     3961 tcgtcgtgaa ctgggtcgcc tacacgcccg acgacatcga gcgcgacctg tcgctcttcg
     4021 aaggaagggt cgggcagtac gtcttcatca gctccgccgc cgtctatcag aagccgcccg
     4081 cccactatct catcaccgaa gagacgccgt tacgaaaccc cttctgggac tacgcgcgcg
     4141 acaagattgc ttgtgaagaa cgtttgctgc gtgcccatgt cgaggacggc ttccccgcga
     4201 cgattgtgcg gccctcgcac acgtacgaga cgaacttccc ggtcgccgtc gggggcggtg
     4261 gtagctacac gctcgccgac cgtttgaaga gagggctgcc cgtcatcgtc cacggcgacg
     4321 gcacctctct gtgggtgttg acgcacgcgg aagacttcgg gcgcgggttt ctggggctgc
     4381 tcgggaaccg cgaggcgatc ggtcaagcct tccacatcac gtcggacgaa gtgctgacgt
     4441 gggagcggat atacgaaacc atcgccgagg cgctcggcgt ggaggcgaac gtcgtgcaca
     4501 tcccgtccga cttcatcgcg cgcgtcgcgc cgggtcgggc ggggagcttg ctcggcgata
     4561 agcagtggag cgccgtcttc gacaacagca agatcaaacg attcgtgccg gagttccgcg
     4621 cggtcatccc ctttcgggag ggcatccggc ggacgctgga gtggttcgag atggacgagc
     4681 gacggcggcg cgtggacgag gggatgcggg aagagatgga gcgcatcctc ggcgcttacg
     4741 gcgcgcgtta tcggagcgtc tgaccgagcc gccgaggctc acagagcgga gagggccatc
     4801 cctgccctct ccgcaccttc ccgctccggg ccgcatgtgg tgtaaactcc tgtcatcaac
     4861 gcttccggga cgcagggtgg tgccgactac acgaatctcc tctgccgcgc gactcacagg
     4921 ccgccccgcc gtccgaacga cccgcccgca gcggcgcagg aatcgaggta ggtaggaagg
     4981 aggcacgtga tgaaccgcat ggtcaagctg gcagcgcggg ggacgacggt gctcctactc
     5041 gcgccggtgt tcgcgctctc cgtcctgggc cggcaaagcg ggacgccccc gcggtcgggg
     5101 gtggatcgcg tgttggccga gcgcgcccga gtcaccaaca acaggaggat agaggaggga
     5161 ctccgcaagc cagccgcgcg cggcgcggag cctcagttgg ccctcgccca gatcaaggaa
     5221 gactacgtgc agattcaggt ggtcacgggc gatctcgcgc gcgcggcttc gcagggcggc
     5281 gcgctcgacc ccaagttcgt cgccaagtcc gcgtcggaga tcaggaagcg cgccgaacgg
     5341 ctcaaacata atctggcgct gcccgaaccg gaacaggccg ccgagcgccc ctcggcggag
     5401 gcgggcgatg aggaggcgcg gctcagggct tccgtctcga ccctcggcaa gttgatcgca
     5461 gggttcgtgc ggaaccccat cttcaggcag gtcggcgtgg ttgacgcccg ttcggcggcc
     5521 acggcgcggc gtgacctcga agagatcatc ggactcagcg gcaggctgaa gaaggagagc
     5581 gagaggttga gcaaggcggt tcagaagtcc cactgaccgc cgttcggcgg gcgcggcgcg
     5641 caattccggg gcgtgtggac acgagcatcc acacgccccg tcatattatt atcttgttac
     5701 attaaaaaat gatgatataa tccccgccgt aaccttcaat cagctccgca aggggattcc
     5761 cacttagggt cattcggggc ggagcctact ctaaacaacg gcagcgcgcg catgcgcccg
     5821 tgggggttca cgcccgcgcg ggcgctcgtc tttgaaggcg acccgctggc cgggccgtag
     5881 ctcacgacat gtcgtctggc cggacggttt tcagaccgcc gaccctctca gcgatgcagg
     5941 aggtgtccaa tgtttatctc gaaatgccgt ttgggtatca gtttcgttat ggtcttcctc
     6001 gtgatggcgt cggcgtcggc ggtcgcgcag gactatcgcg gcaaggtgca gggcacggtc
     6061 gtggacgtga acggcgccgc cgtcgtcggc gccggcgtca cgctgcgtaa cgacgggacg
     6121 ggggtcgcgg tgacgcgcca gacgaacgac gacgggcgct acatcttcga cttcgtcgat
     6181 ccgggtactt acaccgtcac ggtcgagcag acgaactata agaagttcga gcagcagaac
     6241 gtggtcgtcc gcaaccgcgg cgacgtgacg gcggacgcga cgctggaggc cggcggggtc
     6301 gaggacgtgg tgacggtcac ggcgccggca gccgaggtgg agttcaacac cagcggctcg
     6361 gcgctgacga tcgagaacaa gatcgtcgat caactgccga tccgggggcg caacccgtac
     6421 aacatcgcca cgctcgaccc gacggtctcg ccggggacgg gttcgacggc caacgagaac
     6481 cggccttatc accacgcctt cgccaacgac atcgacgccg gcggcgggac gcggagcggg
     6541 aacgacgtgc agctcgacgg cgtcccgctg acctccagct tcaagacctc ttacacgccc
     6601 aacatggacg ccgtgcagga ggtcacgttc cagaagaacg ccgtcgattc ggagtacggc
     6661 tacagctccg gcggcatcgt catcgtgaac acgaagggcg gcacgaacga gttccacggc
     6721 acggccttcg tcaacgggcg cagcccccgc ttcaacgccg tcagcgaccc gaccgtgttg
     6781 cgcatcgcgg ggtcggacga gacgctgcgc cgcgggtcga acctgaagat ttacggcggc
     6841 tccgtcggcg gccccatcct ccggaacaag ctcttcttct tcgccaccta cgagaagtgg
     6901 acggactcgc agcccgcgac cttcatcacg accgtcccca ccgaggccga gcgccggggc
     6961 gacttcagcc agtcgtcgcg cggggtgcgc aacatcttcg acccgttcac ctcgaccggc
     7021 gcgtcagggg tgcgccagca gttcccgggc aacatcatcc ccgcgagcag gttcgacccg
     7081 actgccctaa ggctgctgga ccaaatcccg ctgccgaccc tgcccggcaa cctcaacaac
     7141 cttgaggtca tcaagacgaa taagaccacg tactggaact tctcgacgcg cgtggactgg
     7201 aaccactcgg agcgttggaa gaccttcgtg cgctacggcc agttcaaggc caacctcctc
     7261 gaagagaacc cgaccagcgc cgcgctgctg ccgatcaacg gcagcaaccg ctacggcctc
     7321 agcatcgccg ccgacaccgt ctacacgctc tcttcgacga tggtgctcaa cctgcgcggc
     7381 aggtacaact cgctgaccga cgcctacgcc gccgagccgg cacagctcgg cccggagggg
     7441 ttcgcggcgc tgttccccgg caccaacttc tcgcaacaga tcctcaccgg gccgaacgtc
     7501 tactacccgg gcatcgacat gcaggtcggc ggtaccacct accgcctcgg gcgacccggc
     7561 agggaattct tccaggagcc gcaggggtac ggcggctctg cgcgcctgaa catctatctc
     7621 ggcgatcacg cggtcaagtt cgggggcgag ctgcgggtcg ataaaggcaa gggggcgcgc
     7681 tacgagccgc tcaacttgag attcac
//
LOCUS       DBNGOH010000807         5142 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4152803, whole genome shotgun sequence.
ACCESSION   DBNGOH010000807 DBNGOH010000000
VERSION     DBNGOH010000807.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5142)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5142)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5142
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4152803"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1571)
                     /locus_tag="AC159P_15530"
     CDS             complement(<1..1571)
                     /locus_tag="AC159P_15530"
                     /inference="COORDINATES: protein motif:HMM:NF019135.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140299 - molecular sensor activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ligand-binding sensor domain-containing protein"
                     /protein_id="HIR3696913.1"
                     /translation="MVKRTHKPPSPPSRVKLLSLVARAVLLAFALAHAAARAEQLPIR
                     RYTVADGLAHDQVQRIVRDSRGFLWFCTLDGLSRFDGHRFHNYNKRDGLPHPTVNDLV
                     ESRRDGSYWVATNGGGVARFDPAEDARRVAAARDGEAVAGPDAGGARRVFKTYRVGED
                     SQANIVDVLLEDRDGNLWAGTQGGLFRLRANDIAGGKFERVPLNVPAYAERVIEIGAL
                     AEDAEGNLWVGTIRGLVRRFPDGRTTYHPVQAAQGTDYVNALLTDKGGRLWVAHQSGL
                     YVVNAVPSVSLAAGAIVPARAPPGAVGGSVRLPEAPGEARRYTTAEGLAANYVVTLGQ
                     LSDGGVWVGTRGGVSVFDGGRLRTYAAAHGLGGRVNAVAEDRDGNVWVGTQASGAAKI
                     ARGGFVSYTEGEGLGSPEVVSMFECPNGVVCVVTTKWTVNWFDGEKFSHARLNLPAHV
                     LESSSGRWQIIRDRAGEWWAATGEGLYRFPAVERLEDLARVPPKARYTTREGLADDNI
                     SRLFEDSRGDVWIGS"
     gene            complement(1888..3078)
                     /locus_tag="AC159P_15535"
     CDS             complement(1888..3078)
                     /locus_tag="AC159P_15535"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR3696914.1"
                     /translation="MRIAILGTRGVPASYGGFETFAEQLSTRLVARGHKVTVYCRAHY
                     VSPRQIEFKGVRLKVLPTIRHKYFDTVVHAFLSSLHAAATSRRYDAALICNAANAPFV
                     PFLRAAGVPVAINVDGLEHKRKKWNWLGKNYYRLAERLAVWLPNETVTDARVIQDYYM
                     ARYRAPSTMIAYGAEVERRLDRATVRRWGVEPNRYVLYVSRLEPENNAHLVIEAFKRV
                     RTPHKLLVVGDAPYAEEYINHLRALARRDRRIVFTGFVFGDAYRALQQNAYCYVHATE
                     VGGTHPALLEAMGYGNCVLTLATPENVEAVGEAAITYADVGDLAEKLRDVLRDGAMVN
                     QYRQRAQERVRQRYDWEFVVNEYENLFARMARQPEPHPHTPEPSALGVETSDVGREAR
                     AGKA"
     gene            complement(3167..4393)
                     /locus_tag="AC159P_15540"
     CDS             complement(3167..4393)
                     /locus_tag="AC159P_15540"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR3696915.1"
                     /translation="MRYTVGVLLDPPAVSILAARTGATTSLLHIAIDAHSVGTGLAGN
                     ETYATNLIESLAEIDRENLYTLYVTKRAAVERYAGRWPNFAVRRTLPHSPLVRIPLTL
                     SAELRRRPVDLLHVQFTAPPFAPCPVVSTVHDLSFEHLPQTFKRRSWMQLRLTVRRTT
                     RNAAHIITPSEYSRRDVIETYRVAPERVTVTPEAAAPHFEPASATEIERVRNVYGIRG
                     VYILAVGSIQPRKNLARLIRAYSDLRRARGGSKLPQLVLVGKQAWLYGETLRAIEEHG
                     IRDATLLTGYVPEADLPALYSGALCFVYPSFFEGFGLPPLEAMRCATPVVTGDRTSLP
                     EVVGDAGLLVDPFDTGAIKHAIERLIDDAALRADLAARGRQRALSFDWRDTARMTLQV
                     YRRVISEGPKSVNRNP"
     gene            4453..>5142
                     /locus_tag="AC159P_15545"
     CDS             4453..>5142
                     /locus_tag="AC159P_15545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696916.1"
                     /translation="MSYTPPLRMRSTQDSNATAARLLRTLAPLLFIAAGLLAYSNVAG
                     NYFLSDDFAQIGRVLAGDLSVVWGREHGGFFRPVFILSYVFDVALWGRRPFGFHLTNV
                     LVHALNSFLVSLLARRLFDGAGVAAGRRDAAALGAGLLFLLHPSHTEAVSWISGRADV
                     LATFFGLLSLLFHLSYAQARLSDKRTPRRAPLAISFVLSLLCFALALFAKEAAAFIPL
                     AALLLGARAAAA"
BASE COUNT          739 a         1773 c         1806 g          824 t
ORIGIN      
        1 taggagccga tccacacgtc gccgcgcgag tcctcgaaga ggcgcgagat gttgtcgtcg
       61 gcgagtcctt cgcgcgtcgt gtagcgggcc ttcggtggga cgcgcgcgag gtcttccaga
      121 cgttcgaccg cggggaagcg gtacagccct tcgcccgtcg ccgcccacca ctcgcccgcg
      181 cggtcgcgga tgatctgcca gcgaccggaa ctcgattcga ggacgtgcgc cgggaggttc
      241 agacgcgcgt gggagaactt ctcgccgtcg aaccagttga ccgtccactt cgtcgtgacg
      301 acgcagacga cgccgttcgg gcactcgaac atcgagacca cctcggggct gcccaggccc
      361 tccccctcgg tgtaggagac gaagccgccg cgcgcgatct tcgccgcgcc gctggcctgc
      421 gtgccgaccc agacgttgcc gtcgcggtct tcggccacgg cgttgacgcg accgccgagg
      481 ccgtgcgccg ccgcgtaagt acgcagccgc ccgccgtcga acacgctcac gccgccgcgc
      541 gtcccgaccc acacgccccc gtccgagagc tgcccgagcg tcaccacgta gttggccgcc
      601 agcccttcgg cggtcgtgta gcggcgcgcc tcgcccggcg cctcgggcag gcggacgctc
      661 cctccgaccg cgcccggcgg cgcccgcgcg ggcacgatgg ctcccgcggc gaggctgacg
      721 gaaggcacgg cgttgacgac gtacagcccc gactggtgtg cgacccagag gcgcccgcct
      781 ttgtccgtca ggagcgcgtt cacgtagtcc gtgccctgcg cggcctgcac gggatggtag
      841 gtcgtccgcc cgtcggggaa gcgccgcacg agtccgcgga tggtgccgac ccagaggttg
      901 ccttccgcgt cctctgcgag cgcgccgatt tcgatcacgc gctcggcgta ggcggggacg
      961 ttgagcggga cgcgctcgaa cttgccgccc gcgatgtcgt tcgcgcggag gcggaacagc
     1021 ccgccctgcg tgcctgccca aaggttgccg tcgcggtctt cgagcagcac gtccacgatg
     1081 ttcgcctgcg agtcttcgcc gacgcggtag gtcttgaaga cgcggcgggc gcccccggcg
     1141 tccggcccgg cgacggcctc gccatcgcgc gcggcggcga cgcggcgcgc gtcctccgcc
     1201 gggtcgaagc gcgcgacgcc gccgccgttg gtcgccaccc agtaggagcc gtcgcggcgg
     1261 ctctcgacca gatcgttgac ggtcgggtgc ggcaggccgt cccgcttgtt gtagttgtgg
     1321 aagcggtggc catcgaagcg cgacagcccg tcgagcgtgc agaaccagag gaagccgcgc
     1381 gagtcgcgca cgatgcgctg cacctgatcg tgcgccagcc cgtcggccac cgtgtacctg
     1441 cggatcggca gttgttcggc gcgcgccgcc gcgtgcgcga gcgcgaacgc gagcagcacg
     1501 gcgcgcgcga ccagcgacaa gagcttgacc cgcgacggcg gcgagggagg cttgtgtgtt
     1561 cgcttgacca cgttggcgtg agccgcgcct cgcggcgcgg gcctgagagc ggggtcagac
     1621 ttgctcgaca gggagggagt tttgcacaac tgccgcaaag gttcaatgcc ttcggcggcg
     1681 ggcggcgggg cgccgccgat gagcccttag acgaacggca cggtgacgcg ggcgcaagga
     1741 cttgcgcgcg gcgggcgggg atcagagttt gtgcgaagcg gaattgagga ggtcgttttc
     1801 gtcgaggccg gggcggttgg ggccgccatc gggcacgggc ggcggttcgg ccttcgacct
     1861 gacgtgcgag gcgcgcggcg cgctctctca agccttcccg gcgcgcgcct cgcggcccac
     1921 gtccgaggtc tcgacgccga gggcggaagg ctccggcgtg tgcgggtgcg gctccggctg
     1981 ccgggccatg cgcgcgaaga ggttctcgta ctcgttgacg acgaactccc agtcgtagcg
     2041 ctggcggaca cgctcctgcg cgcgctgccg gtactggttg accatcgcgc cgtcgcggag
     2101 cacgtcccgc agcttctccg ccagatcgcc cacgtccgcg taggtgatcg ccgcctcgcc
     2161 gacggcctcg acgttctcgg gcgtcgccag cgtcagcacg cagttgccgt agcccatcgc
     2221 ttcgagcaac gccgggtgcg tgccgccgac ctctgtggcg tggacgtagc agtaggcgtt
     2281 ctgttgcagc gcgcggtagg cgtcgccgaa gacgaagccg gtgaagacga tgcggcggtc
     2341 gcggcgggcg agcgcgcgca ggtggttgat gtactcctct gcgtagggcg cgtcgccgac
     2401 gaccaagagc ttgtgcggcg tgcgcacgcg cttgaaggct tcgatgacga ggtgcgcgtt
     2461 gttctccggc tcaaggcgcg agacgtagag cacgtagcgg ttcggctcga cgccccagcg
     2521 cctgacggtc gcgcggtcta aacggcgctc gacctcggcg ccgtaggcga tcatcgtcga
     2581 gggcgcgcgg tagcgcgcca tgtagtagtc ctgtatgacg cgcgcgtccg tcaccgtttc
     2641 gttcggcagc cacacggcca gacgctccgc cagccggtag taattcttgc cgagccagtt
     2701 ccacttcttg cgcttgtgct ccagcccatc gacgttgatg gcgacgggga cgcccgcggc
     2761 ccggaggaaa gggacgaagg gcgcgttggc ggcgttgcag atgagcgcgg cgtcgtagcg
     2821 gcgcgaggtg gccgccgcgt gcagcgaaga gaggaaggcg tggacgaccg tgtcgaagta
     2881 cttgtggcgg atggtgggca gcaccttcag gcgcacgccc ttgaactcga tctgccgggg
     2941 gctgacgtag tgggcgcggc agtagaccgt caccttgtgg ccccgcgcga cgagcctcgt
     3001 cgaaagctgc tcggcgaacg tctcgaaccc cccgtagctc gcgggcacac cccgcgtccc
     3061 cagtatcgcg atgcgcatgt gaaccagtga cgaggcgaaa accgtgaacc gtgaaccgtg
     3121 aaccggaaac cgtgagaaga ggaaagcagg tcttccttgt cacggtttac ggattgcggt
     3181 ttacggattt cgggccttca cttatcaccc tcctgtagac ctgtagagtc atgcgggccg
     3241 tgtcccgcca atcgaatgag agcgcgcgtt gtctgccgcg cgccgcgagg tcggcgcgca
     3301 gcgccgcgtc atcgatcagg cgctcgattg cgtgcttgat ggcgcccgtg tcaaaggggt
     3361 caaccagcag gccggcgtcg ccgaccacct ccggcaggct cgtgcggtcg ccggtgacga
     3421 ccggcgtcgc gcagcgcatc gcttcgagcg ggggaagacc gaaaccctca aagaaagacg
     3481 gatagacgaa acagagtgcg cccgagtaaa gcgcgggcaa gtcggcctcc ggcacgtagc
     3541 cggtgagcag ggtcgcgtcg cggatgccgt gctcctcgat ggcccgcagc gtctcgccgt
     3601 aaagccacgc ctgcttgccg accagcacca gttgcggcag cttactcccg ccgcgcgcgc
     3661 ggcgcaagtc cgaatacgcg cggatcaaac gggccagatt cttgcgcggc tggatggagc
     3721 cgaccgccag gatgtacacg ccgcggatgc cgtaaacgtt ccggactctt tcgatttccg
     3781 tcgcggaggc cggctcgaag tgcggcgcgg cggcctcggg cgtgacggtc acgcgctcgg
     3841 gcgcgacgcg gtaggtctcg atcacgtcgc gccgcgaata ctccgaaggc gtgatgatgt
     3901 gcgcggcgtt gcgcgtcgtc cgccgcaccg tcaggcggag ctgcatccag ctccgccgct
     3961 tgaaggtctg cggcaggtgc tcgaacgaga ggtcgtggac ggtcgagacg acggggcacg
     4021 gcgcgaaggg cggggccgtg aactgcacgt gcaaaagatc gacgggcctg cggcgcagct
     4081 ccgccgagag cgtcaagggg atgcggacga gcggcgagtg cgggagcgtg cgacgcacgg
     4141 cgaagttggg ccagcggccc gcgtagcgct cgaccgccgc gcgcttcgtc acgtagagcg
     4201 tgtacaggtt ctcgcggtcg atctccgcca gcgactcgat gaggttcgtg gcgtaggtct
     4261 cgttcccggc cagcccggtg ccgaccgagt gcgcgtctat cgcaatgtgc agcagacttg
     4321 tagtcgctcc cgtgcgcgcc gcgagaatgg aaactgccgg ggggtcaagt aggacgccga
     4381 cagtgtaccg cagaggccgc ccgttcccga atctcgcgcg ccgttcattt caccgccgcg
     4441 gacgaatcga ccatgagcta tactcctccg cttcggatgc gctcgacgca agacagcaac
     4501 gcgaccgccg cgcgactctt aaggacgctc gcgcccctgc tcttcatcgc ggcggggctg
     4561 ctcgcgtatt cgaacgtcgc gggcaactac ttcctctcgg acgacttcgc gcagatcggg
     4621 cgcgtgctcg cgggcgacct ctccgtggtc tgggggcgcg agcacggcgg cttcttccgg
     4681 cccgtcttca tcctcagcta cgtcttcgac gtcgcgctct ggggccgccg ccccttcggc
     4741 tttcacctca cgaacgtcct cgtccacgcg ctcaactcct tcctcgtctc cctgctcgcg
     4801 cggcgactgt tcgacggcgc gggcgtcgcc gccgggcggc gcgacgcggc ggcgctcggc
     4861 gccggtctgc tcttcctgct ccacccctcg cacacggagg ccgtctcctg gatcagcggt
     4921 cgcgccgatg tgctggcgac gttcttcggc ctgctctcgc tcctcttcca tctctcctac
     4981 gcgcaagctc gtctctccga caaacgcacg ccgcgacgcg ccccgctcgc aatatcattc
     5041 gtcctctcgc tcctctgctt cgcgctcgcg ctgttcgcca aagaggccgc ggccttcatc
     5101 ccgctcgccg cccttctgct cggcgcgcgc gccgccgccg ca
//
LOCUS       DBNGOH010000808         3793 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3603029, whole genome shotgun sequence.
ACCESSION   DBNGOH010000808 DBNGOH010000000
VERSION     DBNGOH010000808.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3793)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3793)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3793
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3603029"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1192)
                     /locus_tag="AC159P_15550"
     CDS             complement(<1..1192)
                     /locus_tag="AC159P_15550"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HIR3696917.1"
                     /translation="MSRSIHFADKGVRARTAASEAAVLARLGEIVGAEHVLVDEERVE
                     PYAGDALKEKFPPEAVVLPASAGEISDIMRLANERRFPVTARGGGVGYTGGAVPVEGG
                     IVIGTDRMNRVKEINPDDLYAVTEPGVTTYALQQAVEELGLFYPPDPASYKNSYIGGN
                     IAENAGGIRSAKYGVTKHYVLGLEVVLPTGEIVKTGGRTSKNVVGFDLTGLMCGSEGM
                     LGIITEATLRLVPLPEATRTVRATFRTMTEACACVTHFTRARVTPVAIEVLDRNAIRA
                     VETEFAFGLGAEAGALLLVSVDGSREEVERSARVVEEVVKAGGGYDVIRSESREDEDR
                     LWDVRRALSPAIKKFGTLKFNEDVVVPRSRVPELIERVEETGRRHETFVVNFGHLGDG
                     NIHVN"
     gene            complement(1413..1568)
                     /locus_tag="AC159P_15555"
     CDS             complement(1413..1568)
                     /locus_tag="AC159P_15555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696918.1"
                     /translation="MADENQRNKCARETCGCPAAKDSKYCSEECEDASKVHMMEIGCT
                     CHHPDCG"
     gene            complement(1772..2458)
                     /locus_tag="AC159P_15560"
     CDS             complement(1772..2458)
                     /locus_tag="AC159P_15560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696919.1"
                     /translation="MKRAIVSACAAFLLAAFAAGARAQDTTTTATAPQDARPGEARVP
                     LAQAATAYDAEGREALSASLLTQDLAGAQDAPVRNTRVVVRNLSRLFYTYAQGWATFY
                     DERGVRCGEGLWKLEAFAPDEQVEVDTPGLRLVCRPATWRVVATTLLTRTTDVAKPRD
                     PNAPPPPEGGVTPPSPTTPAASPATAATSGVPPLEININGKTVPIQLGNPLEVTVGNE
                     RVRIVVQPAP"
     gene            complement(2504..>3793)
                     /locus_tag="AC159P_15565"
     CDS             complement(2504..>3793)
                     /locus_tag="AC159P_15565"
                     /EC_number="5.1.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF021387.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase family
                     protein"
                     /protein_id="HIR3696920.1"
                     /translation="LLTTVYFLMSIQLGYGRGHVTFEFEGGRFGVLEGEARDERALSD
                     AEVGAAIDDPFDSQPLEEIISPGESVLVVVSDATRATGSAQFVHILVRRLIQLGVAPY
                     DLRIIFATGIHRRVTREEKQELLSPFIVQRVRTLDHDAADESKHVSLGETERGTPIEL
                     DRALFDHSHVVLTGAVGFHYFAGFTGGRKSVCPGLASARTIEATHLQALDFERGGRRT
                     GVGAGRLDGNAVHEECERVAAEVAPSFLINTVVDEKGRVTRLYAGDWRAAHRLACAEY
                     ADAHTVTIPERRGAVVVSAGGAPHDIDFIQAHKALEMASYACAEGGTLVLVAECADGV
                     GRPDLLKWFDDADSRELETRLCEGYEVNGQTAWSLLTKTERFDVRLVSNLPEEDVRRM
                     RMKPARTIAEALAQSGAGASASGYVMPRGATFMPVVG"
BASE COUNT          560 a         1361 c         1241 g          631 t
ORIGIN      
        1 agttgacgtg gatgttgccg tcgccgaggt ggccgaagtt gacgacgaag gtctcgtggc
       61 ggcggcccgt ctcttcgacg cgctcgatca gctccggcac gcgcgagcgc ggcaccacca
      121 cgtcctcgtt gaacttgaga gtgccgaact ttttgatcgc gggcgagagc gcgcggcgca
      181 cgtcccacag gcggtcttcg tcctcgcgcg actcggagcg gatcacgtcg tagccgccgc
      241 ccgccttcac cacctcttcg acgacgcgcg ccgagcgctc gacctcttcg cgcgagccgt
      301 ccacggagac caggaggagc gcgcccgcct ctgcgccgag gccgaaggcg aactcggtct
      361 cgacggcgcg gatggcgttg cggtccaaga cctcgatggc gacgggcgtg acgcgcgcgc
      421 gcgtgaagtg cgtcacgcag gcgcacgcct ccgtcatcgt gcggaacgtg gcgcgcacgg
      481 tgcgcgtcgc ctcgggcaga gggacgaggc gcagcgtcgc ctccgtgatg atgccgagca
      541 tgccttcgga gccgcacatc aggcccgtca gatcgaaacc gacgacgttc ttgctcgtcc
      601 ggcctcccgt cttcacgatc tcgccggtcg gcagcaccac ttccagcccg agcacgtagt
      661 gcttcgtcac gccgtacttc gccgagcgga tgccgcccgc gttctccgcg atgttgccgc
      721 cgatgtacga gttcttgtac gaggcggggt cgggcgggta gaagaggccc aactcctcga
      781 ccgcctgttg cagcgcgtag gtcgtcacgc ccggctcggt cacggcgtag aggtcgtccg
      841 ggttgatctc cttcacgcgg ttcatgcggt cggtgccgat gacgatgccg ccctcgaccg
      901 gcaccgcccc gcccgtgtag ccgacgccgc cgccgcgcgc cgtgaccgga aagcgccgct
      961 cgttcgccag ccgcatgatg tcggaaatct ccccggcgga cgcgggcagc acgaccgcct
     1021 cgggcgggaa cttctccttc agcgcgtcgc ccgcgtaagg ctcgacgcgc tcctcgtcca
     1081 ccagcacgtg ctcggccccg acgatctcgc cgaggcgcgc cagcacggcg gcctcagacg
     1141 ccgccgtgcg cgcgcgcacc cccttatccg cgaagtgaat cgaacggctc atctcgacag
     1201 tgctgctcct tgccggatac tttatcgtgc gagtcgccgt cgcatcaaca ggaggttgtc
     1261 gttgacgctg atcgcggcca gacgaaggcg cgcgcagcaa gtggtttgcg ccgccgtgct
     1321 gttcatgtaa gacgggtcgc gtgaggcgcg ttcgggaaag gctcggcgcc cgcggccacc
     1381 ggaggcggct gccgcgggcg acgggtggac tcttaaccgc agtcggggtg gtggcaggtg
     1441 cagccgatct ccatcatgtg caccttggac gcgtcctcgc actcctcgct gcaatacttg
     1501 ctgtccttcg ccgcgggaca gccgcaggtc tcgcgcgcac acttgttccg ttgattctcg
     1561 tccgccatct ctgcacgtcc tccttccggc aacttgtagc tgtgggggtt tcgctgtggc
     1621 ctaagtttca acccgacacc cgcgggaagt caagacgact gcggaatgcg gatttcggat
     1681 tgcggattgg aagagcagtg atgagtgata acagcagcgc tgtcgtcact cgtcctcgct
     1741 cctgaaatcc gcaatccgca ttccgcaatt cttacggcgc cggctggacg acgatgcgga
     1801 cgcgctcgtt gccgacggtg acttcgagcg ggttgccgag ctggatgggg acggtcttgc
     1861 cgttgatgtt gatttcgagc ggcggcactc cgctcgtcgc cgccgtcgct ggtgacgcgg
     1921 cgggcgtggt cggcgagggc ggcgtgacgc cgccttccgg gggcggcggt gcgttcgggt
     1981 cgcgcggctt ggccacgtcg gtcgtgcgcg tcaggagcgt ggtcgccacg acgcgccacg
     2041 tcgcgggcct gcacacgaga cgcaggcccg gcgtgtccac ctccacctgc tcgtcgggcg
     2101 cgaacgcttc gagcttccac agcccctcgc cgcagcgcac gccgcgctcg tcgtaaaagg
     2161 tcgcccaccc ctgtgcgtac gtgtagaaga gccggctgag gttcctcacc acgacgcgcg
     2221 tgttgcgcac gggcgcgtcc tgcgcccccg cgaggtcttg cgtcagcagc gacgccgaca
     2281 gcgcctcgcg cccctccgcg tcgtaggccg tggctgcctg cgccagcggc acgcgcgcct
     2341 cgcccggtcg cgcatcctgc ggcgccgtcg cggtcgtcgt cgtatcctgc gccctcgcgc
     2401 ccgcggcgaa cgccgccaac aggaacgccg cgcacgccga gacgattgct cttttcattc
     2461 cccctcctct cgcccgcacc gaagcggcga ctttaacaca cgctcaaccc acgaccggca
     2521 tgaacgtcgc gccgcgcggc atgacgtaac cgctcgcgct tgcgcccgcg cccgactgcg
     2581 cgagcgcttc ggcgatggtg cgcgcgggct tcatccgcat ccgccgcacg tcttcttcgg
     2641 ggaggttcga gacgaggcgc acgtcgaacc gctccgtctt cgtcaggagc gaccaagcgg
     2701 tctgcccgtt cacttcataa ccttcgcaca gccgcgtctc aagctcgcgc gagtcggcgt
     2761 cgtcgaacca cttcaataag tcgggccgcc cgaccccgtc cgcgcactcc gcgacgagga
     2821 cgagcgtgcc gccttcggcg cacgcgtagg acgccatctc cagagccttg tgcgcctgaa
     2881 tgaaatcgat gtcgtgcggc gcgccgcccg ccgagacgac gaccgccccg cgcctctccg
     2941 gaatcgtaac cgtgtgcgcg tcggcgtact cggcgcaggc caaacggtgc gccgcgcgcc
     3001 agtcgcccgc gtagagacgc gtgacacgac ccttctcgtc aaccaccgtg ttgattaaaa
     3061 acgacggcgc gacctccgcc gccacgcgct cgcactcctc gtgcacggcg ttgccgtcga
     3121 ggcggcctgc gccgacgccc gttcgccgcc cgccgcgctc gaagtcgagc gcctgcaagt
     3181 gcgtcgcctc gatcgtcctc gcggaagcga ggccggggca gacgcttttg cgcccgcccg
     3241 tgaagcccgc gaagtagtgg aagccgaccg cgcccgtcag gacgacgtgc gagtggtcga
     3301 agagcgcgcg gtcgagttcg atgggcgtgc cgcgctcggt ctcgcccaga gagacgtgct
     3361 tcgactcgtc cgccgcgtcg tggtcgagcg tgcgcacgcg ctgcacgatg aaaggcgaca
     3421 gcagctcctg cttctcttcc ctcgtcaccc gccggtggat tcccgtcgcg aagatgatgc
     3481 gcaaatcata aggcgcgacg ccgagctgta tcagccgccg cacgaggatg tgcacgaact
     3541 gcgcgctccc ggtcgcgcgc gtcgcgtccg agacgacgac gagcacgctc tcgcccggac
     3601 tgatgatctc ttcgagcggc tgcgaatcga aagggtcgtc aatcgccgcc ccgacctccg
     3661 cgtccgacag cgcgcgctcg tcgcgcgcct cgccttcgag caccccgaag cgcccgccct
     3721 cgaactcgaa cgtgacatga ccgcggccgt agcctagttg gatggacata agaaagtaga
     3781 cagtagtcag tag
//
LOCUS       DBNGOH010000809         6363 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4336295, whole genome shotgun sequence.
ACCESSION   DBNGOH010000809 DBNGOH010000000
VERSION     DBNGOH010000809.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6363)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6363)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6363
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4336295"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..561
                     /locus_tag="AC159P_15570"
     CDS             <1..561
                     /locus_tag="AC159P_15570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019493009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3696921.1"
                     /translation="GLAIVRHLAELHGGTARVESRGENMGTTFSVSLPPSAVLAEERR
                     SRSQLAHAGAASNGHAALEGIRVLVVDDEQDARKVISTVLTQSGAEVHAFESAAEALA
                     EVEWWMPDVIMSDIGMPGEDGYALIKKLRDLPESRGGRIPAAALTAYARDEDRERALA
                     AGFQKHIAKPVRSAELVGVVADLARV"
     gene            905..1417
                     /locus_tag="AC159P_15575"
     CDS             905..1417
                     /locus_tag="AC159P_15575"
                     /inference="COORDINATES: protein motif:HMM:NF024622.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4032 domain-containing protein"
                     /protein_id="HIR3696922.1"
                     /translation="MIFFKLDYVIEMEQAARRDAAQCAAHAKRSGTPAEPNALRSKLV
                     SALKSLANEYLTHQSWLAHNGLFAPTPMASARAGEVVLTERHRNYARELRKLAGVKLS
                     AREADMVWPRLLEHKWYMGERMGRDVGLRVAAADFFENIQPPREHASWRTAWEPLPRL
                     PMMLPFGERA"
     gene            complement(1519..1911)
                     /locus_tag="AC159P_15580"
     CDS             complement(1519..1911)
                     /locus_tag="AC159P_15580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696923.1"
                     /translation="MYKRVLSFALAGLLIHCAGAFMPANAATKEEKRARFAEKVRAGV
                     ATLGTGEAVRVRVKLRDKTKLEGYVAEAGATDFVVVDAKTGASTRVAYAQVKGVQGNN
                     LSTGAKIAIGLGVAVAVLVILLIFENYG"
     gene            complement(2095..3528)
                     /locus_tag="AC159P_15585"
     CDS             complement(2095..3528)
                     /locus_tag="AC159P_15585"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014081.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR3696924.1"
                     /translation="MKINKYLLPLLLLAAFASAGAQPSPRVTVFENVNVIPMDRERVL
                     KNQTVIVRDGVIAEMGDARKVKAPAGASRVDGRGKYLMPGLVDMHTHLFSDDDFPDEL
                     AGDELTLMLANGVTTARLMIGTPEHLILREKIGRGEMLAPTLYVASPQLTGNARYAES
                     NGRHVATPEDARRAVRDFKAAGYDFIKITNFITRPVYDAATEEAKAVGIRIVGHVDTQ
                     VGVARALEAGQQIEHLDAYLESVLKDDSPIKTSVSDIGLFKKENWTSLDHIDERKVRE
                     IARATARAGVYSTPTLTFFKITYGTGATDEEIRAYPDYRFAPPHKRERWERVSKRLRQ
                     DPPTEARRREYVRVRNLLVKEIHAAGGKIMTGSDTPEWYLLYGWTLHRELRSLVEAGL
                     SPYAALEAATRNPAEYLKATDHIGTVARGRRADLLLLEANPLDSIANTERRAGVMSRG
                     RWLPEAELRTMLDAVAVRFEKTAAPAK"
     gene            4002..4838
                     /locus_tag="AC159P_15590"
     CDS             4002..4838
                     /locus_tag="AC159P_15590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696925.1"
                     /translation="MKVGRFARTTLAALALVLASGVVALGDVIRMKDGQVIRGQIIAF
                     RDQQFTILIGSGARGRRSQMTVYMEDVESIEFEAAGAAATTTTTAPDTSDASEPARPQ
                     PQPTNTTRPGPPTLGGRDTGRDNTSAPPDTARDTGAASRPGPSANNSSLIPVRVRVRA
                     DAASNGWTDSGLMVRRGQRLRITAAGRVSLGQGRFSTPTGLPRVVDTEKLMRDKPTGA
                     LIAVVGDDNDDFIFVGSAYEFYATRDGRLFLGVNEGNLDDNTGTYDALVEIEPAASGG
                     DE"
     gene            5038..6306
                     /locus_tag="AC159P_15595"
     CDS             5038..6306
                     /locus_tag="AC159P_15595"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HIR3696926.1"
                     /translation="MKSTTVRRLALAAAVVSLLSASLLAPGLFEAGAQSRRQPPASQS
                     SPQKKNKRPDPADPSRQEQQPEPPPGEILPTKDDEPVKVTTSLVNVEAVVYHKKSGQI
                     VTNLKQGNFQIFEDGIKQEITNFSTPEAPITVAVVLEYSKTAQIMGLYGNQGMERGEL
                     ELIRPTAIFLQQFIRPPDDYVSVVAYDMRPTPLTDFTNDPRRISEVITLLLRNQPASR
                     EANLFDALKLVLVGGRADSVVLENSKEEKSEYAGMISIQRRRRAVFLVSTGLDTFSKI
                     NYDEARKIAQNAGIPIYIVGTGELFMKRYGDDLSATDGLAGSIAPGRLSLLQARNTLR
                     TFADETGGMYLPVTFPGELPNVLQTFNAMMRSQYSLAYNPGERRDGKRHKIIVKVDVD
                     GDGTLDEKEFVVKARQFYNAPKEAKDSSKK"
BASE COUNT         1096 a         2130 c         2120 g         1017 t
ORIGIN      
        1 ggcctcgcca tcgtccgcca cctcgccgag ctgcacggcg gcacggcccg cgtcgagagc
       61 cgcggcgaga acatgggcac caccttttcc gtcagcctcc cgccgtccgc ggtgctggct
      121 gaggagagaa ggtcgcggag tcagctcgcg cacgcgggcg ccgcgtcgaa cggccacgcg
      181 gccctcgaag gcatccgcgt cctcgtcgtg gacgacgagc aggacgcgcg caaggtcatc
      241 agcacggtgc tcacgcagtc gggcgccgag gtgcacgcct tcgagtccgc cgcggaagcc
      301 ctcgcggagg tggagtggtg gatgcccgac gtgatcatga gcgacatcgg gatgcccggc
      361 gaggacggct acgcgctcat caagaagctg cgcgatctgc ccgagagccg cggggggcgc
      421 atccccgccg ccgccctcac ggcctacgcg cgcgacgaag accgcgagcg cgcgctggcc
      481 gcgggcttcc agaaacacat cgccaagccc gtccgctcgg cggaactggt cggcgtcgtc
      541 gcagacctcg cgcgggtgta agagagactc aagggcggct ctaacaaaag gatgttgatg
      601 aaggacggcg ggggttcggc tcccgccgtt ttcgtttgcg acggagcgac gttgccgaag
      661 catggcgcga acatgtccgg agtgttacat gcggggccgg ggagttctaa cgtttatgtc
      721 acaggccggt gcgtgttcct ttgccaccac cacatccttt gtaatcagca cagttacgga
      781 aagtaaaatg cgtttatagg cgggattaaa ataattaatc agcgacaccg cgctgcatcg
      841 cgtaccatct aaagagcggg gcaaagtccc gcgttttatg gatacaggat tcatggaggt
      901 tgcaatgata tttttcaagt tggattacgt catcgagatg gagcaggcgg cgcgtaggga
      961 cgcggcgcag tgtgccgcac acgcgaagcg gagcggcacg cctgcggagc cgaacgcgct
     1021 gcggagcaag ttggtcagcg cgttgaagag tctggcgaac gagtacctga ctcaccagag
     1081 ttggctggcg cataacggct tgttcgcgcc gacgccgatg gcgtccgcgc gcgcgggcga
     1141 agtggtcttg acggagcgac accgaaacta cgcgcgcgag ctgcggaagc tggcgggcgt
     1201 gaagttgtcc gcgcgcgagg ccgacatggt ctggccgcgg ctgctcgaac acaagtggta
     1261 catgggcgag cggatggggc gcgacgtggg gttgcgcgtg gcggcggcgg acttcttcga
     1321 gaacatccag ccgccgcgcg agcacgcctc ctggcgcacg gcgtgggagc cgctcccgcg
     1381 tctgccgatg atgctgccgt tcggcgagcg ggcttgaacg ttccgcacga ccggcggcaa
     1441 tcaacaacag agacgcgcag ggcgcgagga gcgaaacgta ttccgctcct cgcgccctgc
     1501 gcgtcttcgc gtttgcggtc agccgtaatt ctcgaagatg agcaggatga ccagcacggc
     1561 cacggcgacg ccgaggccga tggcgatctt ggcgccggtc gagaggttgt tgccctgcac
     1621 ccctttgacc tgcgcgtagg cgacgcgtgt gctcgcgccg gtcttcgcgt ccacgacgac
     1681 gaagtcggtc gctccagcct ccgcgacgta gccttcgagc ttcgtcttgt cccgcagctt
     1741 gacccgcacg cgcacggcct cgccggtgcc gagcgtggcg acgcccgccc tgaccttctc
     1801 tgcgaagcgc gcgcgcttct cttccttcgt cgccgcgttc gcgggcatga acgctcccgc
     1861 gcagtggatc agcaaacccg cgagcgcgaa cgacagaaca cgtttgtaca tactttcctc
     1921 ctgacgaata gtgggactgc cgacccacaa gcgttgcaac ccgcgcgccc gaggattcgg
     1981 cggcgttagt ttgaatcgag agggaggagc ctgtccacgc ctccgacgaa actacgacac
     2041 ctgaaagcag actgttgcgc gtccctcacc agacaccatt cacgacggtc ggcctcattt
     2101 cgccggcgcg gcggtcttct cgaagcggac ggcgacggcg tcgagcatcg tgcgcagttc
     2161 ggcttcgggg agccagcgcc cgcgcgacat gacgcccgcg cgccgctccg tgttggcgat
     2221 ggagtcgagc gggttggctt cgagcagcaa gaggtctgcg cgtcgcccgc gggcgacggt
     2281 gccgatgtgg tcggtcgcct tcaggtactc ggcggggttg cgggtcgcgg cttcgagcgc
     2341 ggcgtaaggc gagaggcccg cctcgacgag gctgcgcagc tcgcggtgca gcgtccagcc
     2401 gtagagcaga taccactcgg gcgtgtccga gccggtcatg atcttgccgc ccgccgcgtg
     2461 aatctctttg acgagcaggt tgcgcacgcg cacgtactcg cgccgccgcg cctcggtcgg
     2521 cgggtcctgg cgcaggcgct tgctgacgcg ctcccagcgc tcgcgcttgt gcggcggggc
     2581 gaagcgatag tcggggtacg ctcgtatctc ctcgtcggtc gcgccggtgc cgtaggtgat
     2641 cttgaagaag gtgagggtcg gcgtcgagta gacgcccgcg cgcgccgtcg cccgcgcgat
     2701 ctctctgacc ttgcgctcgt cgatgtggtc gaggctcgtc cagttctcct tcttgaagag
     2761 gccgatgtcg gagaccgacg ttttgatcgg cgagtcgtcc ttcagcaccg attcgaggta
     2821 ggcgtcgagg tgttcgatct gctggccggc ttcgagcgcg cgcgcgacgc cgacctgcgt
     2881 atcgacgtgg ccgacgatgc ggatgccgac cgctttcgcc tcttcggtcg ccgcgtcgta
     2941 gacggggcgc gtgatgaagt tcgtgatctt gataaagtcg tagccggcgg ccttgaagtc
     3001 gcgcaccgcg cggcgcgcgt cttcgggcgt cgccacgtgc cgaccgttcg actcggcgta
     3061 gcgcgcgttg ccggtgagct gcgggctggc gacgtagagc gtcggcgcga gcatctcgcc
     3121 gcgtccgatc ttctctcgga ggatgagatg ctcgggcgtg ccgatcatca ggcgcgccgt
     3181 cgtcacgccg ttcgccagca tcagcgtcag ctcgtcgccc gccagctcgt ccgggaagtc
     3241 gtcgtccgag aagaggtgcg tgtgcatatc cacgaggccc ggcatgaggt acttgccgcg
     3301 cccgtccacg cgcgaagcgc cggcgggcgc cttcaccttg cgcgcgtcgc ccatctccgc
     3361 gatcacgccg tcgcggacga taacggtctg gttcttcagc acgcgctcgc gatccatcgg
     3421 gatcacgttg acgttctcga agaccgtcac gcgcggcgac ggctgcgcgc ccgccgaggc
     3481 gaacgccgcg agcagcaaca gcggaagcag gtatttgtta atcttcatcc ggaggctccg
     3541 tggagaaaga tgcagacgca tcggcaatat tgtaggggca gggcttgtcc ctgcccgtcg
     3601 tgcgatggcg cgaagatcga attcaatgtg gccgcgggcc gcggacaagc cctgccccta
     3661 cagtcttcac gcgcgcaccg cactgacacg aaactcattt ccgtttgtga agttaaagca
     3721 gcgggaatca atctcgaacc gcaagacgca gattggcccg gcgccgtatt ttacgccgcg
     3781 cgcgcgcctt cacggaaaga gattgtgacc aacggagtgg aatcggtgac gagacggata
     3841 agcgcgggac gggggcggct gaaactttct ggcaaccaac gggtaagctc gcgccatcca
     3901 tcgcgcgaaa gcacgccgct cccccgcttg ccaaagcgcg tctctctctg ttagatgtcc
     3961 tctgcgaaga ccgattcgcc gttcgtttcc ggaggtgttt catgaaagtt ggaagattcg
     4021 cccgcacgac gctggccgcg ctcgcgctcg tgctggcctc gggcgtcgtc gcgctcggcg
     4081 acgtgatccg catgaaggac ggccaggtca tccgcggcca gatcatcgcg ttccgcgacc
     4141 agcagttcac catcctcatc ggcagcggcg cgcgcgggcg gcgcagccag atgaccgtct
     4201 acatggagga cgtggagtcc atcgagttcg aggcggcggg cgccgcggcg acgaccacga
     4261 cgacggcgcc cgacacctcg gacgcctcgg agcctgcgag gccgcagccg cagccgacga
     4321 acacgacccg gcccggcccc ccgacgctcg gcgggagaga cacgggccgc gacaacacga
     4381 gcgcgccgcc cgacaccgcg cgcgacacgg gcgccgcttc gcggcccggc ccttcggcca
     4441 acaactcttc gctcatcccg gtgagggtgc gcgtgcgcgc cgacgccgcg tcgaacgggt
     4501 ggaccgactc ggggctgatg gtgcggcgcg ggcagcgcct gcgcatcacg gcggcgggcc
     4561 gcgtctcgct cgggcagggg cgcttctcga cgcccacggg cttgccgcgc gtggtggaca
     4621 ccgagaagct gatgcgcgac aagccgacgg gcgcgctcat cgcggtggtc ggcgacgaca
     4681 acgacgattt catcttcgtc ggctcggcct acgagttcta cgcgacgcgc gacgggcggc
     4741 tcttcctcgg cgtcaacgag ggcaacctcg acgacaacac gggcacctac gacgcgctcg
     4801 tcgagatcga gccggcggcg agcggcgggg acgagtaggg cgcgggccgg agggcagcgg
     4861 ccacaagaat aacgaggccg ggggtcggat ttccggggcg ggcgatgaac tctttgcgcg
     4921 ccccggacat ctaaccctcg gcctctattt tgtttgaccg cgcgcgaggg cgggccgtat
     4981 catcggctgg aagaaaatcc gtcttcggcc tccttctcta acaaaggctc tacgtctatg
     5041 aaaagcacta ccgtccgccg tctcgccctc gccgccgccg tcgtctcgct gctttcggcc
     5101 tcgctcctcg cgcccggcct cttcgaagcc ggcgcgcagt cgcggcgcca gccgcccgcc
     5161 tcgcagtcct cgccgcagaa gaagaacaaa cggcccgacc ccgccgaccc ttcgcggcag
     5221 gagcaacagc ccgagcctcc gccgggggaa atccttccga cgaaggacga cgaacccgtc
     5281 aaggtcacga cctcgctcgt caacgtcgag gccgtggtct accacaagaa gagcgggcag
     5341 atcgtgacca acctgaagca ggggaacttc caaatcttcg aggacggcat caagcaggag
     5401 atcaccaact tctcgacgcc cgaggcgccg atcaccgtcg cggtcgtgct cgaatacagc
     5461 aagaccgcgc agatcatggg cctctacggc aatcagggga tggagcgcgg cgagctggag
     5521 ctgatccgcc cgacggccat cttcctccag cagttcatcc gcccgcccga cgactacgtc
     5581 tcggtcgtcg cctacgacat gcggcccacg cctttgaccg acttcaccaa cgacccgcgc
     5641 cgcatcagcg aggtcatcac gctgctgctc cgcaaccagc ccgcctcgcg cgaggccaac
     5701 ctcttcgacg cgctcaagct cgtgctcgtc ggcgggcgcg cagactccgt ggtgctcgaa
     5761 aactcgaagg aggagaagtc cgagtacgcc gggatgatct cgatccagcg ccgccgccgc
     5821 gccgtcttcc tcgtcagcac cggcctcgac acgttcagca agatcaacta cgacgaggcg
     5881 cgcaagatcg cgcagaacgc gggcatcccg atctacatcg tcggcacggg cgagctgttc
     5941 atgaagcgct acggcgacga cctcagcgcg accgacggcc tcgccggctc gatcgcgccg
     6001 ggccgcctct cgctgctgca agcgcgcaac accctgcgca ccttcgccga cgagaccggc
     6061 ggcatgtacc tgcccgtcac cttccccggc gagctgccca acgtcctcca gaccttcaac
     6121 gcgatgatgc gcagccagta cagcctcgcc tacaaccccg gcgagcgccg cgacggcaag
     6181 cgccacaaga tcatcgtcaa ggtggacgtg gacggcgacg gcacgctcga cgagaaggag
     6241 ttcgtcgtca aggcccgcca gttctacaac gcgccgaagg aggcgaagga cagcagcaag
     6301 aagtaagcgt ccccttgcat ccgggaaaga gagaggccgc ggtgaaccgt tcaccgcggc
     6361 ctc
//
LOCUS       DBNGOH010000810         7647 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_853909, whole genome shotgun sequence.
ACCESSION   DBNGOH010000810 DBNGOH010000000
VERSION     DBNGOH010000810.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7647)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7647)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7647
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_853909"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..378)
                     /locus_tag="AC159P_15600"
     CDS             complement(<1..378)
                     /locus_tag="AC159P_15600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696927.1"
                     /translation="MNLTESRLKELDNPSLAGNERVLLRCRLASDFIHAGQYETAREA
                     LGELWQGVGVRPETAGLEPPSAAEVLLQCGVLSGWLGSAQHLPGAQEKAKDLIFEALR
                     GFQSLRQRAKVAEAQFELGMCYFW"
     gene            499..750
                     /locus_tag="AC159P_15605"
     CDS             499..750
                     /locus_tag="AC159P_15605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696928.1"
                     /translation="MKALRALILVLALSVCVYADGNMPNGITTTPPPASATGNMPNGI
                     DGDMGSGDAVVEDSGTSTTTEPTITEAVLSLLQSVLSVF"
     gene            complement(1425..2249)
                     /locus_tag="AC159P_15610"
     CDS             complement(1425..2249)
                     /locus_tag="AC159P_15610"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3696929.1"
                     /translation="MTVSTTNPETEALKTRLKAMWMAGDYGEVAKHIETNAEEFIARL
                     ALKSGARVLDVACGSGNLALPAARAGAVVTGVDIAANLLEQARARAEAEGLSIRFDEG
                     DAESLPYPDASFDVVVSMFGAMFAPRPELVAAELVRVCRPGGLIAMANWTPEGFVGRM
                     FKLGGKYVPPPPGMPPPVKWGDEETVRERLRDGVADLRLTRRICLFDYPFPPAEVVEF
                     FRKYYGPTQRTFDALDDAGQAALRGDLERLWTEHNRATDDTTRVEGEFLEVVATRS"
     gene            2418..2705
                     /locus_tag="AC159P_15615"
     CDS             2418..2705
                     /locus_tag="AC159P_15615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696930.1"
                     /translation="MKRLLGLTLSLASICFVASPAEAKAEPSAASASAAAASTAAPQW
                     RQGRYNRRRARVYTTTRLVRRGRRVYRETYQIRYLPNGRTVTRLISRVRVR"
     gene            complement(2808..3731)
                     /locus_tag="AC159P_15620"
     CDS             complement(2808..3731)
                     /locus_tag="AC159P_15620"
                     /inference="COORDINATES: protein motif:HMM:NF018677.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrase ferredoxin"
                     /protein_id="HIR3696931.1"
                     /translation="MAETSRFYCSELSRRAGERPFGTASVGAVWLLLEYPHGWGRRAL
                     EDSALSAEVKDHFTRACEQIRHSRLLFIKTDRALRDARMNFFVVRCRERSPFVVRFKL
                     KEYDDLTRLDLAAVASASDLQGGQAGADPLFLVCTHGRRDKCCAKFGIPLYNALRDYA
                     GASVWQSSHVGGDRFAGNLLCFPHGLFYAHTTPDAGRRLLSDYREMKVSLDNFRGRAC
                     YAHHTQAAELFVRAEAGIHGVEALRHLSTTREGDASWLVKFAERDGTGVHAARVTRRP
                     STFRNFTTCHSTAEKPVQQFHLEDYRLAPKE"
     gene            complement(3738..4931)
                     /locus_tag="AC159P_15625"
     CDS             complement(3738..4931)
                     /locus_tag="AC159P_15625"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: protein motif:HMM:NF025007.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR3696932.1"
                     /translation="MDSLVKILEPRDAGEFLASAWGTNFMHVRGGAGKFARLMPWARL
                     NEILRRHRLDFPRLRLVQDGEAVPTSSYLRYISGGRRKIPVPRIKPVELARHLREGAT
                     LVLDAVDEFQEPIEELAAGLELTFREHVQVNLYAGWQTSRGFDLHWDDHDVFILQVTG
                     RKRWSVYGQTRPHPLVGDIEKAEKPTHAPVWEGTLEDGDLLYIPRGWWHVAVPLAEPT
                     LHLTVGVHNRNGLDLLRWLAERMRARETFRRDLPRLAAPDERAAHLARMREELLAELD
                     DTILERFYAERDALAEPRARLELPWAATRHAAPVAARSLVRLLAPRPLNFETADGVVE
                     FSAMKKRWRFAADALHVLRPLAERRACTVAELCEAARGRLGEETVRAFVAELSLHGLV
                     SVSEG"
     gene            complement(5086..5463)
                     /locus_tag="AC159P_15630"
     CDS             complement(5086..5463)
                     /locus_tag="AC159P_15630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696933.1"
                     /translation="MADHVQPGATGDEQEVSSSGLLLSVDPLPGTTGYKLSTDDTDAT
                     DADASDTTDVTGGDTDASDASDADASDTIGGDTNASDTSDADGTDLGDSEETESGLGG
                     QNPLGINRPERVKNGDDKDTRDT"
     gene            5794..7314
                     /locus_tag="AC159P_15635"
     CDS             5794..7314
                     /locus_tag="AC159P_15635"
                     /EC_number="3.2.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010342248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0031222 - arabinan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HIR3696934.1"
                     /translation="MTPTRTGAQSAAPFSAELTLRADQPQGTINRNIYGHFAEHLGRL
                     VYDGLWVGEGSPIPNTRGIRNDVVAALKNLSIPVIRWPGGCFADEYHWRDGVGPRAQR
                     PKRINTSWGGVVETNAFGTHEFLDFCEMIGADPYISGNVGSGTPQEMMDWVEYMTSDA
                     DTELANLRRRHGREKPWKIPYFGVGNESWGCGGNMRPEYYADEYRRFGTFVKNYSDNR
                     LQKIASGANAEDYNWTEVLMARAGRQMHGLSVHFYTLPTGDWQKKGSATQFGEAEWHS
                     TLARTLRMDELVRKHGEIMDKHDPEKRVGMMVDEWGTWYDVEPGTARSGLYQQSTIRD
                     ALVAGINLHIFHQHHARVKMANIAQTVNVLQAVILTDGGKMLLTPTYHVFEMYKAHQG
                     ATFVPVELVAPEYKHGAASVPSLHASASRDAGGKLHLSVVNLDPNRPAPVSLTVAGAA
                     AKNVEGRVLTAPAINTVNTFDKPDAVKPAPFTGVELKGGQLTLTLPSKSVVVLEIQ"
     gene            7395..>7647
                     /locus_tag="AC159P_15640"
     CDS             7395..>7647
                     /locus_tag="AC159P_15640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696935.1"
                     /translation="MKITSLVLLLCFAYAAATTVAAQEEVLVKPKLRVRVRGERLVIT
                     ERGRARTLDLGDKVSAARIEDAAVLFKSVSGGAVYLLLDI"
BASE COUNT         1313 a         2521 c         2553 g         1260 t
ORIGIN      
        1 ccagaagtag cacatgccta gctcgaactg tgcctcggca accttcgcgc gctgccgcag
       61 agactggaat ccgcggagtg cctcgaagat taagtcttta gctttctcct gagcgcccgg
      121 cagatgctgc gcactgccga gccatcccga cagcacgcca cattgcagca gcacctcggc
      181 ggcgctcgga ggctccagcc ctgccgtctc ggggcgcacg ccgacgccct gccacagctc
      241 gcccagcgct tcgcgtgccg tttcgtactg ccccgcgtgg atgaagtcgg acgccaggcg
      301 gcagcgcagc aagacgcgct cgttgccggc gagggatggg ttatctagtt ctttcaggcg
      361 gctctctgtt aaattcatgt gagggttgcc tttccgtgtg tttgccatat tctggcaaag
      421 acgccgaagc cgccgcgcct cacgaaagcg cgacggcggc actggtcgaa tcctgtcaac
      481 aacgaaagga aaagagcgat gaaggctctc agagcgttaa tacttgtgtt ggccctttcc
      541 gtctgtgtct acgccgatgg gaacatgccc aacgggatca ccactacgcc gcccccggcg
      601 tccgctaccg gaaacatgcc caacggtatc gacggggaca tgggcagcgg ggatgccgtg
      661 gtagaggaca gcggtacgtc taccacgacg gaacccacca tcaccgaggc cgtcctgagc
      721 ctactgcaaa gcgtgctgtc cgtcttctag gcggcgcaca ccaatgcaaa cgattatagc
      781 tgccgtgaac ggctcgactg gcggcgcggt ctttgtgtat ctaaggagag tgcgaaaagc
      841 ccggggcggc ggcaaggtga cactcacagc gggcaagata acgcggatgc gagaagcatt
      901 gcaacccaat tcttctgccc gcccggtagc tggtccgtgt gtgaaaagcg cgtcactcag
      961 gcgcccgacc gagtatcagc gccgggccac ggcccccgag gccggaacac acaccgctcg
     1021 tcggtattgg tcaggtaagc gacaacaagc aaatccgact ttgcaatacc tgttgcaaag
     1081 tggtctaacc cccagccggt cttagcgata cgaggcggct gattgtgttc gggttgacag
     1141 actcgccaac ctgactagaa ttacgcgcaa tcgggcggct ggtgaaccct acccgcccag
     1201 tggttgctgg ggtgaggaca gtgaccacct ttagagcggg cgcgaggctc ccaaagctga
     1261 cgcgcccgtt catatttgcc gttgccgttg ccgcccgcct tgcgcacacc gtccagcgtg
     1321 atgacatcga acccccgcga ggaggcgtct tgagagaagt gctgtcgggg cggtcagccg
     1381 ggacgacacg tcgcgggcgg ctgacaggga aaggctttag accttcaact cctcgtcgct
     1441 acgacttcga ggaactcgcc ctcaacccgc gtcgtgtcgt cggtcgcacg attgtgctcg
     1501 gtccacagcc gctcaaggtc gccgcgcagc gcggcctgcc cggcgtcgtc gagcgcatcg
     1561 aacgtgcgtt gcgtcgggcc gtaatatttg cggaagaact cgacgacctc ggcgggcggg
     1621 aacgggtagt cgaacagaca aatccgccgc gtcagtcgta agtccgcgac gccgtcgcgc
     1681 agccgttcgc gcacggtctc ctcgtcgccc cacttgacgg gcggcggcat gccgggcggc
     1741 ggcggcacat acttgccgcc gagcttgaac atccgaccga cgaacccttc gggcgtccag
     1801 ttcgccatcg cgatcagccc gccgggccgg cagacgcgca ccagctcagc cgcgaccagc
     1861 tcgggccgcg gcgcgaacat cgccccgaac atactcacga ccacgtcgaa ggacgcgtcg
     1921 gggtagggca ggctctccgc gtcgccttcg tcgaaccgaa tcgacaagcc ttcggcctcg
     1981 gcccgcgccc gcgcctgttc gagaaggttc gcggcgatgt ccacgccggt gacgacggcg
     2041 cccgcgcgcg ccgccggcag cgccagattg ccgctgccgc aagccacgtc gagcacgcgc
     2101 gcgcccgact tcagcgcgag gcgcgcgata aactcctcgg cgttcgtctc gatgtgcttc
     2161 gcgacctcgc cgtaatcgcc ggccatccac atcgctttga gtcgggtctt cagagcttcc
     2221 gtctcgggat tcgtcgtgct cacagtcagt ctcctttcga gtcgtagaga gtctcacgga
     2281 taaataaaca ctttccggaa aaaattgtaa cccctcaccg gtaacctgtg tcttacttat
     2341 taggccggac tctcccgccg ggcgttccga acgcggcgcg gagtgccggc ctgatttttt
     2401 tcgggaagga gaaggaaatg aagagattac tgggactgac actctccttg gcttcaatct
     2461 gtttcgtcgc ttcgcccgcg gaggcgaagg ctgaaccgtc cgccgcctcg gcgtccgccg
     2521 ccgcggccag cacagccgcc ccacagtggc ggcagggccg ttataacagg cggcgcgccc
     2581 gcgtctacac gacgacgcgc ctcgtgcgcc ggggccggcg agtttaccgc gagacgtacc
     2641 agatcagata cctgccgaac ggcaggactg tgacgcgact catcagccgc gtcagagtcc
     2701 gctaagcacg acgcacggat tcgatggccg gcgtgcccga gatgattctc tcgggcacgc
     2761 cggccatcgc tttttttatt ccgcgaatca tcggcgcgcg agaaggctca ctccttcggg
     2821 gcgaggcggt agtcttcgag gtggaactgt tggacgggct tctcggcggt cgagtggcag
     2881 gtcgtgaagt tgcggaacgt ggaggggcgg cgtgtgacgc gcgcggcgtg gacgccggtg
     2941 ccgtcgcgtt cggcgaactt gacgagccaa gaggcgtcgc cctcgcgcgt ggtcgagagg
     3001 tggcgcagcg cctcgacgcc gtggatgcct gcctcggcgc ggacgaacag ctcggcggcc
     3061 tgcgtgtggt gcgcgtagca ggcgcgcccg cggaagttgt cgagtgacac cttcatctcg
     3121 cggtagtccg agagcaggcg gcggcccgcg tcgggcgtcg tgtgcgcgta gaagaggccg
     3181 tggggaaagc agaggaggtt gccggcgaag cggtcgccgc cgacgtgcga cgactgccag
     3241 accgacgcgc ccgcgtagtc gcgcagggcg ttgtagagcg ggatgccgaa tttggcgcag
     3301 cacttgtcgc gcctgccgtg cgtgcagacg aggaagagcg ggtcggcgcc ggcctgtccg
     3361 ccctgaaggt cgcttgcgga ggcgaccgcc gcgaggtcga ggcgcgtgag gtcgtcgtac
     3421 tctttcagct tgaagcggac gacgaagggc gagcgctcgc ggcagcggac gacgaagaag
     3481 ttcatgcgcg cgtcgcgcag cgcgcggtcg gtcttgatga acagcaggcg cgagtggcgg
     3541 atctgttcgc acgcgcgcgt gaagtggtct ttgacctcgg cggagagggc gctgtcttcg
     3601 agcgcgcggc ggccccagcc gtgcgggtat tcgagcagca gccagaccgc gccgaccgag
     3661 gcggtgccga aaggtctctc gccggcgcgg cgcgagagtt cggagcagta gaagcgcgag
     3721 gtctctgcca aagcccctca cccttcggag acggacacga gtccgtgcaa agacaactcc
     3781 gcgacgaagg cgcgcacggt ctcttcgccg agcctgccgc gcgccgcctc gcacaactcg
     3841 gcgacggtgc acgcgcgccg ctccgcgagc ggtcgcagca cgtgcagcgc gtccgcggcg
     3901 aagcgccagc gctttttcat cgcggagaat tcgacgacgc cgtccgccgt ctcgaagttc
     3961 agcgggcgcg gcgcgagcag ccgcacgagc gagcgcgccg ctacgggcgc cgcgtgccgc
     4021 gtcgcggccc acggcagttc gaggcgcgcg cgcggctcgg ccagagcgtc gcgttcggcg
     4081 tagaagcgtt cgaggatcgt gtcgtccagc tcggcgagaa gctcttcgcg catccgcgcg
     4141 aggtgcgcgg cgcgctcgtc gggcgcggcg aggcgcggca ggtctcggcg gaaggtctcg
     4201 cgcgcgcgca tgcgctcggc gagccagcgc agcagatcga ggccgttgcg gttgtggacg
     4261 ccgacggtga ggtggagcgt cggctcggcg agcggcacgg cgacgtgcca ccagccgcgc
     4321 gggatgtaga gcaggtcgcc gtcttcgagc gtgccctccc agacgggcgc gtgcgtcggc
     4381 ttctcggcct tctcgatgtc gcccacgagc gggtgcgggc gcgtctggcc gtagacgctc
     4441 cagcgcttgc gccccgtcac ctggaggatg aacacgtcgt gatcgtccca gtgcaggtcg
     4501 aagccgcgcg aggtctgcca gcccgcgtag aggttgacct gcacgtgctc gcggaaggtc
     4561 agctcaagcc ccgcggccag ctcctcgatc ggctcctgaa actcgtccac ggcgtcgagc
     4621 acgagcgtcg cgccctctcg caggtggcgc gcgagttcga ccggcttgat gcgtgggacg
     4681 ggaatcttcc gccgcccgcc cgagatgtat cgcaggtagg aagaggtggg caccgcctcg
     4741 ccgtcctgca cgagccgcag gcgcgggaag tcgagccggt ggcggcgcag tatctcgttc
     4801 aaacgcgccc acggcatgag acgcgcgaac ttgcccgcgc cgccgcgcac gtgcatgaag
     4861 ttcgtgcccc acgccgaggc gaggaactcg cccgcgtcgc gcggctcaag gattttcacc
     4921 aggctgtcca caaaatgcgg aaggcaaaag gcaagaggca aaagttaaaa ggcaaaaggg
     4981 cgggcatgcc tgcggcgtga actcttcaaa tcgaaaatcg aagcgcagct tcgtccgccc
     5041 ttttgccttt tgcctcttgc cttttgcctc ttgccttttg ccttctcagg tatctctcgt
     5101 gtccttgtcg tcgccgttct tgacgcgctc ggggcggttg atgccgagcg ggttctgtcc
     5161 gccgaggccc gactcggtct cttcggagtc gccgaggtct gtgccgtccg cgtccgaggt
     5221 gtcggacgcg tttgtgtcgc cgccgatggt gtcggaggcg tccgcgtccg aagcgtcgga
     5281 ggcgtccgtg tcgccgcccg tcacgtccgt cgtgtcactc gcgtccgcgt ccgtggcgtc
     5341 cgtgtcgtcg gtgctcagct tgtagccggt ggtgcccggc agtggatcga ccgaaagtag
     5401 aagacccgaa gaagacactt cctgctcgtc gcccgttgcg ccgggctgca cgtgatctgc
     5461 catgcccttc ctccttaccg aattgtggaa ctgaatttcc cacagcttag tacgccgggt
     5521 gcgacgagga caagcggcga cggcgaaagt cgcgcgccgt cgaaaggaac tctctacagc
     5581 ggcgccgccg tgcgcccccg aacgtcccgc cgaaaaagat tgagttttgc cgcggatgtc
     5641 ggtaaaagtt ggcagcagcc tttaacgagc cgacacacct tgaggagttg gagaatgaag
     5701 agaacgactg ttcccgcggt ctgtacgggc cgcgcgccgc gggcggcgtt ggcggccacg
     5761 ctgtccgcct gtcttctcgt cgtggtgatg atgatgacgc cgacacgcac cggcgcgcag
     5821 agcgccgcac ccttcagcgc cgagctgacg ttgcgcgcag accagccgca gggcacgatc
     5881 aaccgcaaca tctacggcca cttcgccgag cacctcggaa ggttggtcta cgacggcctc
     5941 tgggtcggcg aaggctcgcc catcccgaac acgcgcggca tccgcaacga tgtcgtcgcg
     6001 gcgctgaaga acttgagcat ccccgtcatc cgctggccgg gcggctgctt cgccgacgag
     6061 taccactggc gcgacggcgt cggcccgcgc gcgcagagac cgaagcgcat caacacgtcg
     6121 tggggcggcg tcgtcgagac caacgccttc ggcacgcacg agttcctcga cttctgcgag
     6181 atgatcggcg ccgaccccta catcagcggc aacgtcggct cggggacgcc gcaggagatg
     6241 atggactggg tcgagtatat gacctcggac gcggacaccg aactcgccaa cctgcgccgc
     6301 cgccacgggc gcgagaagcc ctggaagatt ccctacttcg gagtgggcaa cgagtcgtgg
     6361 ggctgcggcg ggaacatgcg ccccgaatac tacgccgacg agtatcgccg cttcggcacc
     6421 ttcgtcaaga actactcgga caaccgcctc cagaagatcg cgagcggcgc caacgccgag
     6481 gactacaact ggacggaagt cctgatggcg cgagccgggc ggcagatgca cggcctctcc
     6541 gtccacttct acacgctgcc gacgggcgac tggcagaaga agggttcggc gacgcagttc
     6601 ggcgaagccg agtggcactc gacgttggcg cgcacgctcc gcatggacga actcgtccgc
     6661 aagcacggcg agatcatgga caagcacgac cccgagaagc gggtcgggat gatggtggac
     6721 gagtggggca cgtggtacga cgtggagccg ggcaccgccc gctccgggtt gtaccagcag
     6781 agcaccatcc gcgacgcgct cgtcgccggc atcaaccttc acatcttcca ccaacaccac
     6841 gcccgcgtga agatggccaa catcgcgcaa accgtgaacg tccttcaggc cgtgatcctg
     6901 accgacggcg ggaagatgct cctcacgccg acctaccacg tcttcgagat gtacaaggcg
     6961 caccagggcg cgacgttcgt cccggtcgag ctggtcgcgc cggagtacaa acacggcgcg
     7021 gcgtcggtgc cttcgctgca cgcctcggcc tcgcgcgacg ccggcggcaa gctccacctc
     7081 tcggtcgtca acctcgaccc gaaccgcccc gcgccggttt cgctgacggt cgcgggggcc
     7141 gccgccaaaa acgtcgaagg gcgcgtgctg acggcgccgg ccatcaacac cgtcaacacg
     7201 ttcgacaagc ccgacgccgt caagcccgcg ccgttcaccg gtgtcgagct gaagggcggc
     7261 caactcacac tgaccctgcc gtcgaagtcg gtcgtcgtgc tggagattca atgagccggg
     7321 ccgcgttcgc ttcgtctctc cgcgacgccg cgggttcaaa cgcggcgtcg cggaagagag
     7381 tcatgcgcag ctttatgaag atcacttcgc tcgttctcct cctttgcttc gcgtacgcgg
     7441 ctgcgacgac cgtcgccgcg caggaggagg tgctcgtcaa gccgaagctc agggtgcgcg
     7501 tgcgcggcga gcgtctcgtc atcacggagc gcgggcgggc gcgcacgctc gacctcgggg
     7561 acaaggtcag cgcggcgcgg atcgaggacg cggcagttct cttcaagtcc gtctccggcg
     7621 gcgcggtcta tctgctgctc gacatct
//
LOCUS       DBNGOH010000811         3799 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1405499, whole genome shotgun sequence.
ACCESSION   DBNGOH010000811 DBNGOH010000000
VERSION     DBNGOH010000811.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3799)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3799)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3799
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1405499"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            58..2745
                     /locus_tag="AC159P_15645"
     CDS             58..2745
                     /locus_tag="AC159P_15645"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR3696936.1"
                     /translation="MPVATGAQLGPYEILSPLGAGGMGEVYRARDTRLGREVAVKVLP
                     PSLSENAERLARFEQEACAAGALNHPNILAIHDVGAHDGKPYVVSELLEGETLREKMG
                     GGALPPRKAVDYALQVARGLAAAHEKGIVHRDLKPENLFVTKDGRVKILDFGLAKLTG
                     GGDGSRAQSDVPTRRVDTDPGAVMGTVGYMSPEQVRGRAVDHRSDIFSFGVILYEMLT
                     GRRAFRGESAADTISAILREDPPDITSSNGAVSPALERLVRRCMEKSPAERFQSARDL
                     AFALEAFSGSNARGQEAVTAVASPPARAKWRERLPWLVAGLLALVAALAFTVPYFLRP
                     PQPEEVRAVRLTIPPPEKGVISTLSVSPDGRRVVYGAPDAEGKVVLWVRPLDSLNAQP
                     LPGTDSPFFSLFWSPDSRSVGFVSSADNKLKKIDVTGGLPQTLADAPNGRGGTWNRDG
                     VIVFAPSSEGPLYRVSASGGEATPVTTLDESRREVSHRFPYFLPDGRHFLYFSRITGT
                     GSMDIYVGSLDSKETKRLVSASSGAMYAPPGHLLYLRDGILVAQGFDANKLELRGEAV
                     PVAEAVIGYDLTSNFARFSVSENGVLVYYAGSEFDKVQLAWVDREGKQTGAVGQPGNY
                     GYLRLSRDDKRLAFALRERQLDIWLMELARGNLMRFTFAPANEVSPVWSSDGTRVAFA
                     SNRSGQIDLYQKLSNGVGNDEELLKSDRPKFPNDWSPDGRFIAYHILSGHTRADIWIL
                     PLDGDRQPFPFLQTPAGEREARFSPDGRWVAYTSDETGPREVFVQSFPAGRGKWQISR
                     GGGGPPRWRSDGKELFYANGTKLMAVAVKPGAETFEAGTPTLLFEIRGASSALPGAGG
                     AAAFDASADGQRFLVALPVEEATSTPITVVLNWTADLKR"
     gene            complement(2780..>3799)
                     /locus_tag="AC159P_15650"
     CDS             complement(2780..>3799)
                     /locus_tag="AC159P_15650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR3696937.1"
                     /translation="LTHRNIVANLCQFKGAGFDWADDTLLCVLPMFHIYGLVAVLNYG
                     LYCGATIITLPRFDLEQALGVMQDYKVSIAHLVPPVVLALAKHPVVERYDLSSVKTVF
                     SGAAPLGEQLSRACCERLGCRIIQGYGMTETSPATHLTSHEHSKHGSVGLPVPYTECR
                     IVCLETGVELGTGAAGEICIRGPQVMKGYLGRPEATAETIDEEGWLRTGDIGYADDEG
                     FFFIVDRAKELIKYKGFQVAPAELEALLLTHPSVADAAVIPSPDEEAGEIPKAFVVLK
                     NGASATAEDAATAEELISYVAARVAPHKKIRLLEFVEQIPKSPSGKILRRTLVQKERE
                     RRAGN"
BASE COUNT          594 a         1289 c         1286 g          630 t
ORIGIN      
        1 ggagcgagta tattatgccc gctcgcgccg aaaactccac agcggattac ccaggtcatg
       61 cccgtcgcca ccggcgcaca actcggcccc tacgaaatcc tctcgccgct cggcgcgggc
      121 ggcatgggcg aggtctaccg cgcccgagac acgcggctcg ggcgcgaggt cgccgtcaaa
      181 gtcctccccc cttccctctc cgagaacgcc gaaaggctcg cgcgcttcga gcaggaagcc
      241 tgcgccgcgg gcgctttgaa ccaccccaac attctcgcca tccacgatgt cggcgcgcac
      301 gacggcaagc cctacgtcgt ctccgaactg ctcgaaggcg agacgctgcg cgagaagatg
      361 ggcggcggcg ccctcccgcc gcgcaaggcg gttgactatg ccctccaggt cgcgcgcggg
      421 ctggcggcgg cgcacgagaa gggcatcgtc caccgcgacc tgaagccgga gaatctcttc
      481 gtcacgaagg atggccgggt caagattctg gacttcgggc tggcgaagct cacgggcggc
      541 ggggacggca gccgtgcgca gtcggacgtg ccgacacggc gcgttgacac ggaccccggc
      601 gcggtgatgg gcaccgtcgg ttacatgtcg ccggagcagg tgcgcgggcg cgcggtggat
      661 caccgctcgg acatcttctc tttcggcgtg atcctctacg agatgctgac ggggcggcgc
      721 gccttccgcg gcgagtcggc ggcggacacc atcagcgcga ttctcagaga agacccgccc
      781 gacattacat cgtcgaacgg ggcggtttct ccggcgctcg aaagactcgt gcgccgttgc
      841 atggagaaaa gcccggcgga gcgttttcag tcggcgcgcg acctcgcctt cgcgctcgaa
      901 gccttctccg gctcgaacgc gcgcgggcag gaggcggtga ccgccgtcgc ctcgccgccc
      961 gcgcgcgcga agtggcgcga gcgcctgccg tggctcgtcg ccggtctcct cgcgctcgtc
     1021 gccgcgctcg ccttcaccgt gccttacttc ctccgcccgc cccagccgga ggaggtgcgc
     1081 gccgtgcgcc tgaccatccc gccgccggag aaaggggtga tctcgacgct cagcgtctcg
     1141 cccgacgggc ggcgcgtggt ctacggcgcg cccgacgcgg agggcaaagt cgtcctctgg
     1201 gtgcgcccgc tcgactcgct caacgcgcag ccgttgcccg gtacggacag ccccttcttc
     1261 agccttttct ggtcgccgga cagccgctcc gtcgggttcg tcagcagcgc cgacaataag
     1321 ctgaaaaaga tcgacgtgac gggcgggttg ccccagacgc tcgccgacgc gcccaacggt
     1381 cgcggcggga cctggaaccg cgacggcgtc atcgtcttcg cgccctcctc ggaggggccg
     1441 ctctatcgcg tgtccgcttc gggcggcgag gcgacgcccg tcacgacgct cgacgaatcg
     1501 cgccgcgagg tctctcaccg cttcccgtac tttctgcccg acgggcgtca cttcctctac
     1561 ttcagccgca tcacggggac gggcagcatg gacatttacg tcggctcgct cgactcgaag
     1621 gagacgaagc gcctcgtgag cgcgagttcg ggcgcgatgt acgcgccgcc cggccacctg
     1681 ctctacctgc gcgacgggat tctggtggcc caggggttcg acgcgaacaa gctcgaactc
     1741 aggggcgagg cggtgccggt cgccgaagcc gtgatcgggt acgacctgac cagcaatttc
     1801 gcgcgcttct ccgtttcgga gaacggcgtg ctggtctatt acgcgggcag cgagttcgac
     1861 aaggtccagc tcgcatgggt tgaccgcgag ggcaagcaga cgggcgcggt cgggcagccg
     1921 ggtaactacg gatacctccg gctctcgcgg gacgacaagc ggttggcgtt cgcgttgcgc
     1981 gagcgacagc ttgacatctg gctgatggaa ctcgcgcgcg gcaacctcat gcgcttcacc
     2041 ttcgcccccg ccaacgaggt ctcccccgtc tggtcgtctg acgggacgcg cgtcgccttc
     2101 gcgtcgaacc ggagtgggca aatcgatctc taccagaagc tttcgaacgg cgtgggtaat
     2161 gacgaggaac tgctcaagtc cgacagaccg aaattcccga acgactggtc gccggacggg
     2221 cgctttatcg cctaccacat cctgtccggg cacacgcgcg ccgacatctg gattctgccg
     2281 ctcgacggag accgacagcc cttccctttt ctccagactc ccgccggcga aagggaggcg
     2341 cgattctcgc ctgacggtcg ctgggtggcc tacacgtccg acgagacagg cccgcgcgag
     2401 gtgttcgtgc agagttttcc cgcggggcgt ggcaagtggc agatttcgag gggcggtggc
     2461 ggccccccgc gatggcgcag cgacggaaaa gagttgttct acgcgaacgg gacgaagctt
     2521 atggcggtcg cggtgaagcc cggcgcggag accttcgaag cgggcacgcc gacgctcctc
     2581 ttcgagattc gtggcgcgtc gtccgcgtta cccggtgcgg gcggcgcggc ggctttcgac
     2641 gccagcgccg acggacagcg cttcctcgtc gccctccccg tcgaagaggc gacctccacg
     2701 ccgatcaccg tcgtcctcaa ctggacggcg gacttgaagc gatgacgggt gacgctccgg
     2761 cacgcctccc ctgccttcgt caattccctg ctctccgctc cctctccttc tgcacgagcg
     2821 tgcggcgcag aatcttcccc gaaggggact tcggaatctg ctcgacgaat tcgagaagtc
     2881 ggattttctt gtgcggcgcg acgcgggcgg cgacgtagga gatcaactct tcggcggtcg
     2941 ccgcgtcttc agccgtcgcg gaagccccgt tcttgagcac gacgaaggct ttcggaatct
     3001 cgcccgcctc ttcgtcggga ctcgggatga cggcggcgtc cgcgaccgag gggtgcgtca
     3061 gcaggagcgc ttcgagttcg gcgggcgcga cctggaagcc cttgtacttg atgagttcct
     3121 tcgcgcggtc aacgatgaag aagaagccct cgtcgtcggc gtagccgatg tcgcccgtgc
     3181 ggagccagcc ttcctcgtcg atggtctcgg cggtcgcctc ggggcgtccc agataaccct
     3241 tcatcacctg cgggccgcgg atgcagattt cgcctgcggc gccggtgccg agttcgacgc
     3301 ccgtctcaag gcagacgatc cggcattcgg tgtagggcac gggcaggccg accgagccgt
     3361 gcttgctgtg ctcgtgcgaa gtgaggtgcg tggccgggct ggtctcggtc atcccgtagc
     3421 cctgaatgat gcggcagccg agccgctcgc agcacgcgcg cgagagctgc tcgcccaggg
     3481 gggccgcgcc ggagaagacg gtcttgaccg acgacaggtc gtagcgctcg acgaccgggt
     3541 gcttcgccag cgcgaggacg acgggcggga cgaggtgcgc tatcgacacc ttgtagtcct
     3601 gcatcacgcc gagcgcctgt tcgaggtcga agcgcggcag cgtgatgatc gtcgcgccgc
     3661 agtagaggcc gtagttgagg acggcgacca gcccgtagat gtggaacatc ggcagcacgc
     3721 acagcagcgt gtcgtcggcc caatcgaagc ccgcgccctt gaactggcac aggttggcga
     3781 cgatgttgcg gtgcgtcag
//
LOCUS       DBNGOH010000812         3485 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4154006, whole genome shotgun sequence.
ACCESSION   DBNGOH010000812 DBNGOH010000000
VERSION     DBNGOH010000812.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3485)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3485)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3485
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4154006"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..347
                     /locus_tag="AC159P_15655"
     CDS             <1..347
                     /locus_tag="AC159P_15655"
                     /inference="COORDINATES: protein motif:HMM:NF012422.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HIR3696938.1"
                     /translation="CKDEPAELVLKAVRKVYEGEIWFDRQTAFGLVAEMIRDGRLDDA
                     QAERLASLTPKEREIIAVISSDEDSTNRQVARRLGISENTLRNHLSSIYDKLGVANRL
                     ELLKFAISQKLA"
     gene            1530..3428
                     /locus_tag="AC159P_15660"
     CDS             1530..3428
                     /locus_tag="AC159P_15660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696939.1"
                     /translation="MSTSAVRSARTYKAPSGGDKAAPSQTAKAAPANTALAKPAGAAN
                     FACDPNNPATCTDVTSVFELADGNTVDAGANAIDDWDTLHAGGGSAVVQTTGTPDPDS
                     PAVNDPKGQSIFTQGGSKDDLDILQWRHTDGNVPDKDEIVNAGAALYITPMNEQILVF
                     FADRFTTNGDAQIGFWFFQDTVSRNMDGSGTFDGQHQIGDILVLSNFTNGGTQPNIRV
                     YRWDPTDPNAENGTLVLIGSAGACNAPAIPGVPKLGCAEVNSGALAGSPLWPFLSKQG
                     ATNYAQAGSFFEGGVNLTALLPAGASVCLSSFLAETRSSASTDATLKDLVGPSSFPAG
                     AEAVAGPDRVIDCTAVDGQITLHGSSSTPGVTLNWTTSDGNIVSQMNVATGPGETAAR
                     DAVVDAAGTYTLTATTGTGCTGSDDVVVTVDLEPPGVMASKSNDLDCDTTSATLSATS
                     PVMASFEWFGPSPDGGVTPGPSIGTGAMINVMAPGIYTVVATGTGNGCTSSAQVSVGQ
                     DITPPSLTIEKTAEDGTNLNVSLGLSAGSAASYQWQSCVAMDCSNSLMNANWVNVAGQ
                     MTSSLSQFSNFELDTPSAISFQVNGVGPMFVGRLWVVNLRLVGTGANGCVAPSNVITV
                     QRVNGVDP"
BASE COUNT          656 a         1180 c         1043 g          606 t
ORIGIN      
        1 tctgcaagga cgagcctgcc gaactggtgc tgaaggcggt cagaaaagtg tacgagggcg
       61 agatctggtt cgaccggcag accgccttcg gcctcgtcgc cgagatgatc agggacgggc
      121 gtctggacga cgcacaggcc gagcggctcg cgtcgctcac gccgaaggag cgcgagatca
      181 tcgccgtcat ctcctcggac gaggattcca cgaaccggca ggtggcgcgc cgcctcggca
      241 tcagcgaaaa caccctgcgc aaccacctct cgtccatcta cgacaagctg ggcgtcgcca
      301 accggctcga acttttgaag ttcgccatca gccagaaact cgcctgacct tccgcaccct
      361 cacgattcga ctgatagtct caccccgcgg tgcccgcctc tcgacggcag gcccctcgcc
      421 gcacgcacga gcgcgcggcc aacgtccaca gcccgccgca tcccctgaac cggcactttt
      481 ctcccgcccg cgccgcgcca aattgggagc tatgtgcctc tccgtttagt acctcctgct
      541 catgcgctgc gggcggcgcg gcgcgtatag tgtcgccaag ctacagctgt ccgcggaaga
      601 gtcgggcgcg tcaaagggtt gccaaggggc gcgtccggtg tgggcgttcg gattgagagt
      661 cgcagattta tttttaggcg cccatttctc aaacgcgttc ttgatcaacg aatgctaaac
      721 cgtttcgggg gcgggcggtt tgcccgccgt gaaccatgca gagagactcc gcaagatcgg
      781 cgccagggcg ccaccgcatc ctaatgtcac cccgatagcc ttcccgcctg accccgccca
      841 tctgcctgac ggtctcagga aaaggagtag atcaagtgcg cccccatgag gtaatggata
      901 atcgccttct ccattggaga tttttggaaa tgtttggtgc ggccttaagg acttttggcg
      961 cgcgcgtgga tgatgatgca cgcgccgtgc tctgcggcga ccgggccggg cggcgtcccg
     1021 tgatcgcgcg ggccggtacc ggcctcgcat aacttttcgg cccttccgcc accgctaatg
     1081 gcggaagggc tctgacgaca cgacccctgc cgcacttccg tgcggaagat ggcgcatcgc
     1141 agcaagccca tcttatcgca ggacgtggca gcggctcaaa ggaaatattt tggggcgctc
     1201 ccatttcacc tgtagctcac aagcagcggg atgtagggtc atcacccacc ctacgtcgac
     1261 ctcgccgcac tatcctctct tgccctccca aacacgcctc ttcacgacaa cccaccaacc
     1321 ggttaattac cacaagggcc agcgggccac ccccgcacca ggatgcaact cgccgggggc
     1381 gaccccccga gaggcggttc ctgccgaagc cgtcggctga ccccttttcc ccggaacatt
     1441 ccggaaggag gatcaagctc atgttatccc gtagtaagcg tcgaactctc attgccctcg
     1501 ctgcagttgc ggttttccta gtcgcaacaa tgagcacctc ggcagttcgc tctgcgcgga
     1561 catacaaagc gccgtcaggc ggcgacaaag ccgcgccgtc ccagacggcg aaggcggcac
     1621 ccgccaacac cgccttggcg aagccggcgg gcgccgccaa cttcgcttgc gaccccaata
     1681 acccggcgac atgtaccgac gtgacctccg tgttcgaact ggcggacggc aacacggtgg
     1741 acgccggcgc taacgccatc gacgactggg acacgctcca cgcgggcggc ggcagcgccg
     1801 tcgtccagac gacgggcacc cccgaccccg actcgccggc cgttaacgat ccgaagggtc
     1861 aatccatttt cacccagggc ggctcgaagg acgacctgga catcctccag tggcgacaca
     1921 ccgacggcaa cgtccccgac aaggacgaga tcgtcaacgc cggcgcggcc ctctacatca
     1981 ccccgatgaa cgaacagatc ctcgtcttct tcgccgaccg cttcaccact aacggcgacg
     2041 cccagatcgg cttctggttc ttccaggaca cggtctcgcg caacatggac ggctccggga
     2101 ccttcgacgg acagcaccag atcggtgaca tcctcgtcct gagcaacttc acgaacgggg
     2161 gcacgcagcc caacatcagg gtctaccggt gggacccgac cgacccgaac gccgagaatg
     2221 ggacgctcgt gctgatcggc tcggctgggg cttgtaacgc gccggccatc ccgggcgtac
     2281 cgaaactcgg ttgcgcggag gtgaactctg gggcgctcgc cggatcgccg ctgtggccgt
     2341 tcctgtcgaa gcaaggcgcc accaactacg cgcaagcggg ttccttcttc gagggcgggg
     2401 tcaacctgac cgcgcttctt cccgccgggg cttcggtctg cctctcgagt ttcctcgccg
     2461 agacccgctc ctcggcctcg accgacgcca ccctgaagga cttggtcggg ccgtcctcat
     2521 tcccggccgg ggccgaggcg gtggcgggac ctgaccgggt gattgactgt acggcagtgg
     2581 acggtcagat cacgctccac ggctcctcct cgacgccggg ggtcaccttg aactggacca
     2641 ccagcgacgg caatatcgtc tcgcagatga acgtcgccac aggtccgggt gagaccgcgg
     2701 cacgagacgc cgtggtagac gccgccggta cttacacctt aacggcgacg accgggacag
     2761 gctgtactgg gagcgatgat gttgtagtca cggtcgacct cgaaccgccg ggcgtcatgg
     2821 caagtaagag caacgacctg gattgcgaca ccacttcggc gacgctcagc gccacatcgc
     2881 ccgtcatggc gagctttgaa tggttcggac cgagtcccga cggcggagtg acgcccggac
     2941 cttcgattgg aaccggtgcg atgatcaatg tcatggcccc gggcatctac acggtcgtgg
     3001 ctaccgggac gggtaacggc tgcaccagct cggcccaggt gagtgtcggt caagacatta
     3061 cgccaccgtc actgaccata gagaagacag ccgaagacgg caccaacctc aacgtgtccc
     3121 tcgggctctc ggccgggtca gccgcttcct accagtggca gtcctgtgtg gcgatggatt
     3181 gctccaactc tctcatgaac gcgaactggg tcaacgttgc aggtcagatg acctcttcgc
     3241 tgtcgcagtt ctccaacttt gagctggaca ctcccagcgc aatctcgttc caggtaaacg
     3301 gagtgggtcc catgtttgtg ggacgactgt gggtcgttaa tctgcgtctg gtcggcaccg
     3361 gcgctaacgg ctgcgtcgcg ccttctaatg tgataacggt ccagcgggtg aacggggtcg
     3421 acccgtaaac ctgttccgac cgcggcgaca gaagggcgcc ggccagaaca atctggccgg
     3481 cgccc
//
LOCUS       DBNGOH010000813         5419 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_122050, whole genome shotgun sequence.
ACCESSION   DBNGOH010000813 DBNGOH010000000
VERSION     DBNGOH010000813.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5419)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5419)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5419
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_122050"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..487)
                     /locus_tag="AC159P_15665"
     CDS             complement(<1..487)
                     /locus_tag="AC159P_15665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012845053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB small GTPase domain-containing protein"
                     /protein_id="HIR3696940.1"
                     /translation="MKAILESPAPAEAAVVAREERSLLVALAGNPNAGKTSLFNALTG
                     LRQKVANYPGVTVERKEGVWQLSAEAAPARLIDLPGLYSLDAASIDERIAHDVLTGRV
                     QEVPAPDVIVAVVDATNLERNLYLVTQLLEIGRPLVVALTMIDLAEREGIEVDAGSLG
                     AA"
     gene            complement(548..805)
                     /locus_tag="AC159P_15670"
     CDS             complement(548..805)
                     /locus_tag="AC159P_15670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013110951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HIR3696941.1"
                     /translation="MNEKTSAKATLDSLPLNARARVVAVRGSGAVARRLMEMGMVPGA
                     PVCVVRSAPLGDPLEVRVRNYHLALRRSEAQTIDVMMSDED"
     gene            complement(1134..2738)
                     /locus_tag="AC159P_15675"
     CDS             complement(1134..2738)
                     /locus_tag="AC159P_15675"
                     /inference="COORDINATES: protein motif:HMM:NF022750.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HIR3696942.1"
                     /translation="MLRLIKSPAPFHALLLVLLLCASARAQQVRSDVASSPFNAAPYV
                     VGEKLSYNVSFSNFPTAAHVELLVAGRGQFSGREGVELRAHVETIGVVSAALYSINND
                     YVTFVDPLTGLPYRAQQTIREGARSEDVSNDLNVGALGSPAMPSTQTSTAPGTYDFLS
                     ALYRLRALPLAPGAVYAINVQHGAKLYNAELRVTGREMVKTNVGSANAIVTQVRVRGD
                     SAADDHRIRVYFTDDERHVPVLITLRLRSGEVRAELASSEILSATPPLAEGPTAPQPQ
                     PTPRANTLPPPGVMPGVTPPGTSAATNPPTGAAPLPALPFKVGEELNFNFFLGNGAQP
                     IGTASFRVRARARYFNRDGLLLSASMRTTGAAQELFQVNDQFNSYVDAATLLPFRTEL
                     SLLEGKRQSKWVVSLDQNGGAALFDDGTRVEMPVNTHDLISVFYALRSFDLTPSKRTA
                     VSLLVNKRQRPLYISALSRATIQLGGQQIPAVELAITTGEAQADSLALRLWVSTDQRR
                     LPLRLTAQTPLGPVRADLAIIPTTLQ"
     gene            complement(2847..3584)
                     /locus_tag="AC159P_15680"
     CDS             complement(2847..3584)
                     /locus_tag="AC159P_15680"
                     /inference="COORDINATES: protein motif:HMM:NF012770.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR3696943.1"
                     /translation="MSVEYRKQGSGPPIVYVSGIEGTGKLFYKQATDLERDHTVISYP
                     LRGEGRYGMERLVEDLAWVVRDAGLERATFVGESFGGLLTMAAALAEPRMFERVILVN
                     TFPFFAQRAKINLGVLLFSLLPYQMIKAHRTRNARHVLFTEDVSDEDRRLFREHTRTV
                     PYEGYVSRLRIIRDTDLRPRLGEIEAPVLVVAGTADRLIDSVGAARVLVERLPRARLK
                     LLEGTGHTALLSGRVSVREWLSEFEDL"
     gene            complement(3876..4655)
                     /gene="pyrF"
                     /locus_tag="AC159P_15685"
     CDS             complement(3876..4655)
                     /gene="pyrF"
                     /locus_tag="AC159P_15685"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012302395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HIR3696944.1"
                     /translation="MTTAPMMEGVTAKDKLIVALDVETAEEARRVYAALREHAGMFKV
                     GSQLFTAAGPPLVRELVAAGARIFLDLKFHDIPNTVAAAAREAVRLGVALFNVHAAGG
                     SEMLRRAAAATAEEAARLGTPRPRLIAVTVLTSADASVLAETGVAAPSIEAHAARLAR
                     LADAAGLDGVVATPHQIAPVRAAVPRRDFLLVTPGVRTASAAHDDQKRVMSPAQAVLA
                     GADFIVMGRAILNAPDPARAAREVVGEIAGSGQQAAGSESM"
     gene            complement(4801..5346)
                     /locus_tag="AC159P_15690"
     CDS             complement(4801..5346)
                     /locus_tag="AC159P_15690"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3696945.1"
                     /translation="MESAYESSPLIHTERLTLRAPRMDDAAAIFDSYARDPEVTRYLV
                     WTPHRSVAETEEYLRGVTTVQGGDPKYHWAVTERGDDTARGMIALRRNGHKADFGYVL
                     ARRLWGRGYMTEALRAVLAFAFTLPGLYRVWAVCDVENAASARVMEKAGLRFEGVLCR
                     HTVHPNIDPEPRDVRSYAITR"
BASE COUNT          846 a         1720 c         1959 g          894 t
ORIGIN      
        1 gcgccgcgcc caggcttccc gcgtcaacct cgatcccttc gcgctcggcg aggtcgatca
       61 tcgtgagcgc gacgacgagc ggacgaccga tctcaagaag ctgcgtgacg agatagaggt
      121 tgcgttcgag gttggtggcg tccacgaccg cgacgatcac gtcgggcgcg ggcacctctt
      181 ggacgcggcc cgtcaacacg tcgtgcgcga tgcgctcgtc tatcgaagcg gcgtcgaggc
      241 tgtagaggcc gggcagatcg atcagcctcg cgggcgccgc ctcggcactc agttgccaca
      301 cgccctcctt gcgctcgacc gtcacgcccg gatagttcgc caccttctgc ctgagtccgg
      361 tcagcgcgtt gaagaggctc gtcttgcccg cgttcgggtt gcccgccaga gcgaccagca
      421 ggctccgctc ctcgcgcgcc acgacggcgg cctcggcggg ggcaggggat tcgagaatag
      481 ctttcataga tacctaagtg atgagtgatg aggactgagt gaaaagctta ttcctttcac
      541 tcagtcctca gtcctcatca ctcatcatta cgtcaatcgt ctgcgcttcg ctgcgccgga
      601 gcgcgaggtg gtagttgcgg acgcggactt cgagcgggtc gccgagcggg gccgacctga
      661 cgacgcagac cggcgcgccg ggcaccatcc ccatctccat cagccgccgc gcgaccgcgc
      721 ccgaaccgcg caccgcgacc acgcgcgcgc gcgcgttcaa cggcagacta tcgagcgtcg
      781 ccttcgcgga tgttttctcg ttcaaagtcg ctcctgaagt ctcctagcgt ggcgccgagc
      841 ctttcggtcg cacacgccaa atccgacggg gacaggatat agcagttgaa attagaattc
      901 aatttcaact agggcgggtc ggagtttcag ggattgtgaa gttcattcga gtcgcgcgaa
      961 tagattggag gggcagggca agttcgcggc ccgcggccac gcgttcactc agcgttgaat
     1021 tacatcgaaa gttgaggggc cgaggacaag tcctgcccct acagtcttct tgcatgtgcc
     1081 gcgtccggcg aggcgtgcgc tgtcgggacg ggcgttgaag tcggacggct gtgtcactgt
     1141 aaggttgtcg ggatgatggc gaggtctgcg cggacggggc cgaggggcgt ctgcgctgtg
     1201 agtcgcaagg ggagtcggcg ctggtctgtg ctgacccaga ggcgcagcgc gaggctgtcg
     1261 gcctgcgcct cgccggtggt gatggcgagt tcgacggcgg ggatttgctg gccgccgagt
     1321 tggatggtgg cgcggctgag cgcgctgatg tagagcgggc gctggcgttt gttgacgagc
     1381 agggagacgg cggtgcgttt cgacggcgtg aggtcgaagg agcgcagcgc gtagaagacc
     1441 gagatgaggt cgtgcgtgtt gacgggcatc tcgacgcgcg tgccgtcgtc gaagagcgcg
     1501 gcgccgccgt tctggtcgag cgagacgacc cacttgctct gccgcttgcc ttcgagcagg
     1561 ctcagctccg tgcggaaggg gagcagcgtc gccgcgtcca cgtacgagtt gaactggtcg
     1621 ttgacctgga acagctcctg cgccgcgccg gtggtgcgca tcgaggcgga gagcagcagg
     1681 ccgtcgcggt tgaagtagcg cgcacgcgcg cggacgcgga acgaggcggt gccgatgggc
     1741 tgcgcgccgt tgccgaggaa gaagttgaag ttcagctcct cgccgacctt gaagggcagc
     1801 gccggcagcg gggccgcgcc ggtcggaggg ttggtcgcgg cggacgtgcc gggcggcgtg
     1861 acgccgggca tgacgccggg cggcggcagc gtgttggcgc gcggcgtcgg ctggggctgg
     1921 ggcgccgtag gcccttcggc gagcggcggc gtcgcggaaa gaatctcgga gctggcgagt
     1981 tcggcgcgca cctcgcccga gcgcaggcgc agggtgatga gcacgggcac gtgccgctcg
     2041 tcgtccgtga agtagacgcg gatgcggtgg tcgtccgccg ccgagtcgcc gcgcacgcgc
     2101 acctgcgtga cgatggcgtt cgcggagccg acgttggtct tcaccatctc gcgccccgtg
     2161 acgcgcagct cggcgttgta gagcttggcg ccgtgctgca cgttgatggc gtagacggcg
     2221 ccgggcgcga gcggcagcgc gcgcaggcgg tagagcgccg agaggaagtc gtaggtgccg
     2281 ggcgcggtgc tggtctgtgt ggacggcatg gcggggctgc cgagcgcgcc gacgttgagg
     2341 tcgttcgaca cgtcctcgga gcgcgcgccc tcgcggatgg tctgctgcgc gcggtagggg
     2401 agtccggtca ggggatcgac gaaggtgacg tagtcgttgt tgatcgagta gagcgcggcg
     2461 ctgacgacgc cgatggtttc gacgtgcgcg cgcagctcga cgccctcgcg cccggagaac
     2521 tgcccgcgcc cggcgaccag cagctcgacg tgcgcggcgg tcgggaagtt cgagaaggag
     2581 acgttgtagg agagcttctc gccgacgacg tagggcgcgg cgttgaaggg gcttgaggcg
     2641 acgtcgctcc tgacctgctg cgcgcgggct gaggcgcaca agagtaaaac cagcagcagc
     2701 gcgtgaaacg gcgccggcga cttgatcagt cgaagcaaaa agttcccctc ctcaaaattc
     2761 gaaacctcgc ggcccgccga ggtcaggggc gggctgtggt ttcacgtcgg cggtgcggtc
     2821 gccgttccaa aagctcccgg cgccgattat aaatcttcga actcgctcaa ccactcgcgc
     2881 acgctgacgc ggcccgacag cagcgccgtg tgccccgtcc cttccaagag cttgaggcgc
     2941 gcgcgcggca ggcgctcgac cagcacccgc gccgcgccga cgctgtcgat caggcggtcg
     3001 gcggtgcccg cgacgacaag cacgggcgct tcgatctcgc cgaggcgcgg gcgcaagtcc
     3061 gtgtcgcgga tgatgcgcag gcgcgagacg tagccttcgt agggcaccgt gcgcgtgtgc
     3121 tcgcggaaga ggcggcggtc ttcgtcgctc acgtcttcgg tgaagagcac gtggcgcgcg
     3181 ttgcgcgtgc ggtgcgcctt gatcatctgg tacggcagca gcgagaagag cagcaccccc
     3241 aggttgatct tcgcgcgctg tgcgaagaaa gggaaggtgt tgaccaggat cacgcgctcg
     3301 aacatccgcg gctcggcgag cgccgccgcc atcgtcagca gtccgccgaa cgactcgccg
     3361 acgaacgtcg cgcgctcaag ccccgcgtcg cgcaccaccc acgcgaggtc ttcgacgagc
     3421 cgctccatcc cgtagcggcc ctcgccgcgc agcgggtacg agatgaccgt gtggtcgcgc
     3481 tcaaggtcgg tcgcctgctt gtaaaagagc ttgcccgttc cctcgatccc cgacacgtag
     3541 acgatgggcg ggccgctgcc ctgtttgcga tactcgacgc tcatgcggaa aagcagtagg
     3601 cagtaggcag taagcagtag gcagcaaaaa ccagcaggca acaggcagcg ggcagcgggc
     3661 agcaggcagc ggaaacgagg tcccggcctc cgtgttcgct cgccggtttc ggctttgcga
     3721 ccgattcggg atttgcgatt cgaaatttcg aattcaaaat cataaatgtt ttgactgctc
     3781 gctgcccctc gcctgctgct tgcttgttgt tactgcctgc tgcctgctgc ccgctgccca
     3841 ctcgttgtta ctgcctactg cctaccgcct actgcctaca tgctttcact gcctgctgcc
     3901 tgctgcccgc tgcccgctat ctctccgacg acctctcgcg cggcgcgcgc ggggtcgggg
     3961 gcgttgagga tggcgcggcc catgacgatg aagtcggcgc cggcgaggac ggcctgcgcg
     4021 ggcgacatga cgcgcttctg gtcgtcgtgc gcggcggagg cggtacgcac gccgggggtg
     4081 acgaggagga agtcgcggcg cggaacggcg gcgcgcacgg gggcgatctg gtggggcgtg
     4141 gcgacgacgc cgtcgagtcc cgccgcgtcc gcgagccgcg cgagccgcgc ggcgtgcgcc
     4201 tcgattgaag gcgcggcgac gccggtctcc gcgagcaccg aggcgtcggc gctcgtgagc
     4261 acggtgacgg cgatgaggcg cgggcgaggt gtgccgaggc gcgcggcctc ttcggcggtc
     4321 gcggcggcgg cgcggcgcag catttcgctt ccgcccgcgg cgtggacgtt gaagagcgcg
     4381 acgccgaggc gcacggcctc gcgcgcggcg gcggcgacgg tgttcgggat gtcgtggaac
     4441 ttgaggtcga ggaagatgcg cgcacccgcg gcgacgagtt cgcggacgag cggcggcccc
     4501 gccgcggtga agagctgcga gccgaccttg aacatgcccg cgtgttcgcg cagggcggcg
     4561 tagacgcgcc gcgcctcctc ggctgtctcc acgtcgagcg cgacgatcag tttatctttc
     4621 gccgtgaccc cttccatcat cggtgcagtc gtcaaacgaa gtctccctcg ggaactcgat
     4681 cttgaacaca cgaacttaac gactcggact cgagcacgac cttgaaaaca tggccgcgca
     4741 cgaaggctcg atcttgaaac accgtcgcgg gacttaacgt gtcatcgcgc cgacgcatcg
     4801 tcaccgcgtt atcgcgtagc tgcgcacgtc gcgcggctcg gggtcgatgt tcgggtggac
     4861 tgtatggcgg cacagcacgc cctcgaaccg caggcccgcc ttctccatga cgcgggcgga
     4921 agccgcgttc tccacgtcgc acacggccca gacgcggtac aggccgggca gcgtgaaggc
     4981 gaaggcgagc acggcgcgca gcgcttcggt catgtagccg cggccccaaa gccggcgcgc
     5041 gagcacgtag ccgaagtcgg ccttgtgccc gttgcggcgc agcgcgatca tcccgcgcgc
     5101 ggtgtcgtcg ccccgctccg tgacggccca gtgatacttc gggtcgccgc cttgaacggt
     5161 cgtgacgccg cgcaggtact cttccgtctc cgcgacggag cggtgcggag tccagacgag
     5221 gtagcgcgtc acctccgggt cgcgcgcgta cgaatcgaag atcgccgcgg cgtcgtccat
     5281 gcgcggggcg cgcaacgtca gacgctcggt gtggatcaac ggactgctct cgtacgccga
     5341 ctccatcggt tatcacgaac gcgcgcggcg tcttatcaaa tgcgcgaggc tcaggccgcc
     5401 gcaagctctt cggcgcggc
//
LOCUS       DBNGOH010000814         3975 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3421707, whole genome shotgun sequence.
ACCESSION   DBNGOH010000814 DBNGOH010000000
VERSION     DBNGOH010000814.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3975)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3975)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3975
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3421707"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2334
                     /locus_tag="AC159P_15695"
     CDS             <1..2334
                     /locus_tag="AC159P_15695"
                     /inference="COORDINATES: protein motif:HMM:NF039587.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate binding family 9 domain-containing
                     protein"
                     /protein_id="HIR3696946.1"
                     /translation="AFKAFDEPDKVRSTVAKRDAVFDDDWVGVWLDTFNDGRRAYELI
                     FNPLGVQADAIFTEGVNEDFSVDIVHESKGSLTTDGFVVEIAIPFKSLRYTAGKDKLW
                     GLHLIRTIKRYNNEQSSWMPISRDNSSLLGQRGYITGLEGVSAERTLEIIPSLTVSET
                     GTRVNSPVPGSPIPTGRTTAPPLDRLLNKPVEFDPGVTTKFTLTPQVTLDFTYNPDFA
                     QVEADATVVTANQRFPIFFEEKRPFFLEGKEIFETLISAVHTRAIVDPDYAVKLTGKQ
                     GKHTFGIMAASDKAPGNLDDDDRDFIARTINTEDQDEALKRDSLVKLLDRNATIGVVR
                     LKRDIGKENHIGLLATTYNFVDKYNHVIGGDARWRLNKTTTFTAQVMGSVSHQPFFYP
                     EEAGRFDRKETGMVYGAYLNMNGRNWGYELGAVGRSKRFRADVGFNRRFDTNNPTLFV
                     RYQSTDKPKQMLVAWRAYNFFGSNFDWKGRHQRYVNESQFQLRMQRQTFIGLGFEKGY
                     ERVFEDEFGGTRAAISRRNREEFAPLVGAGRVSALPGCDQTGGLAPVIPDDPATEEDE
                     TRRLGPCTFFGDDNERSADRTSFYWYVETRPSKKYSIFVFSSYNHGVLDFDFGSGSPR
                     YRRVSPAALAFGQDAPLDPGAGNEFFLESTFNFQPTDELRMSLLYTKDRLSRHDTGLL
                     AFDDNIFSLRGTYQFTRFWFARARVDYTTLSSRMRMQYLLGWTPNPGTQFYVGYNDDL
                     NRNGFNPFNGDLEPGFRRNGRVFFIKMSYLIRKSFGN"
     gene            2523..>3975
                     /locus_tag="AC159P_15700"
     CDS             2523..>3975
                     /locus_tag="AC159P_15700"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009768794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HIR3696947.1"
                     /translation="MRRQVRLIVAALLLASTVAAQGGMIVPGDNLVVEGVPAIPANIA
                     EEVRRYTEFRAANLSSWHPTKREMLISTRFGDTNQVHHVKMPGGARTQLTFFPERSGG
                     AAYRPKTGDYFVFNKDIGGNEFFQFYRYDVATGDVTLLTDGKSRNTGGTWSHGGEWLA
                     YQSTRRNGKDNDIYLVNPAQPSSNKLLIEVEGGGWGVSDWSPDDKKLLVGEFISANES
                     YGYVVDVATGEKTLLTPKGGEKVAYGGAQFAKDGKGVYVTTDKESEFQRLAYIDLATK
                     QHTYLTDHIKWDVDDFELSPDGSTIALVTNEDGISRLRLLDTKTRKEKTPPELPVGLV
                     GALEWHDNGRDLGFSLSSARSTSDVYSLDVKTGKVERWTFSETGGLNTDNLSDPQLVK
                     WKSFDGREISGFLYLPPAKFTGPRPVIINIHGGPEGQARPGFIGRNNYFLNEQGVSII
                     YPNVRGSVGFGKTFVGLDNGMKREDSVKDIGALL"
BASE COUNT          755 a         1424 c         1174 g          622 t
ORIGIN      
        1 gccttcaagg cgttcgacga gccggacaag gtgcgctcca cggtggccaa gcgcgacgcc
       61 gtcttcgacg acgactgggt cggcgtctgg ctcgacacct tcaacgacgg gcgccgcgcc
      121 tacgaactga tcttcaaccc actcggcgtt caggccgacg ccatcttcac cgagggcgtg
      181 aacgaagact tcagcgtgga catcgtgcac gagtcgaagg gttcgctcac gacggacggc
      241 ttcgtcgtcg agatcgccat cccgttcaag tcgctgcgct acacggcggg caaggacaag
      301 ctctggggcc tgcacctcat ccgcaccatc aagcgctaca acaacgagca gagttcgtgg
      361 atgccgatct cgcgtgacaa ttcgagcctg ctcgggcagc gcggctacat caccggcctc
      421 gaaggcgtct ccgccgagcg caccctcgaa atcatcccga gcctgaccgt ctccgagacc
      481 ggcacgcgcg tcaactcgcc cgtccccggc tcgcccatcc cgacgggccg cacgacggcg
      541 ccgccgctcg accgcctgct caacaagccg gtcgagttcg accccggcgt caccaccaag
      601 ttcaccctca cgccgcaggt cacgctcgac ttcacctaca accccgactt cgcgcaggtc
      661 gaggccgacg ccaccgtcgt caccgccaac cagcgtttcc ccatcttctt cgaggagaag
      721 cgccccttct tcctcgaagg caaggagatt ttcgagacgc tcatcagcgc cgtgcacacg
      781 cgcgccatcg tcgatccgga ctacgccgtc aagctcacgg gcaagcaggg caagcacacc
      841 ttcggcatca tggcggcctc ggacaaggcg cccggcaacc tcgacgacga cgaccgcgac
      901 ttcatcgcgc gcaccatcaa cacggaggat caggacgagg cgctgaagcg cgactcgctc
      961 gtcaagctgc tcgacaggaa cgcgaccatc ggcgtcgtcc gcctcaagcg cgacatcggc
     1021 aaggagaacc acatcgggct gctggcgacc acctacaact tcgtcgataa gtacaaccac
     1081 gtcatcggcg gggacgcgcg ctggcgcctc aacaagacga cgaccttcac cgcgcaggtg
     1141 atgggcagcg tctcgcacca gcccttcttt tatccggagg aggccgggcg cttcgaccgc
     1201 aaagagacgg gcatggtcta cggcgcctac ctcaacatga acgggcgcaa ctggggctac
     1261 gagctgggcg ccgtcgggcg ctcgaagcgc ttccgcgccg acgtgggctt caaccgccgc
     1321 ttcgacacca acaacccgac cctcttcgtc cgctaccaat cgaccgacaa gccgaagcag
     1381 atgctcgtcg cgtggcgcgc ctacaacttc ttcggctcga acttcgactg gaaggggcgg
     1441 caccagcgct acgtcaacga gtcgcagttc cagctccgca tgcagcgcca gaccttcatc
     1501 ggcctcggct tcgagaaggg ctacgagcgc gtcttcgagg acgagttcgg cggcacccgc
     1561 gccgccatct cgcgccgcaa ccgcgaggag ttcgcgccgc tcgtcggcgc ggggcgcgtg
     1621 agcgcgctgc ccggctgcga ccagacgggc ggcctcgcgc ccgtcatccc ggacgacccg
     1681 gcgaccgaag aggacgagac gcgccgcctc ggcccgtgca ccttcttcgg ggacgacaac
     1741 gagcgctccg ccgaccgcac gagcttctac tggtacgtcg agacgcgccc ctcgaagaag
     1801 tactcgatct tcgtcttcag ctcctacaac cacggcgtcc tcgacttcga cttcggctcg
     1861 ggcagcccgc gctaccggcg cgtcagcccg gcggcgctcg ccttcggcca ggacgccccg
     1921 ctcgaccccg gcgccggcaa cgagttcttc ctcgaatcga ccttcaactt ccagccgacc
     1981 gacgagctgc ggatgtcgct cctctacacg aaagaccgcc tcagccgcca cgacacgggc
     2041 ctgctcgcct tcgacgacaa catcttctcc ctgcgcggca cctaccagtt cacgcgcttc
     2101 tggttcgcgc gcgcgcgcgt ggactacacc acgctcagct cgcgcatgag gatgcagtac
     2161 ctcctgggct ggacgccgaa ccccggcacg cagttctacg tcggctacaa cgacgacctc
     2221 aaccgcaacg gcttcaaccc cttcaacggc gacctcgaac ccggcttccg ccgcaacggt
     2281 cgcgtcttct tcatcaagat gtcgtacctc attcgcaaga gcttcggaaa ctgataagag
     2341 ccgtgaaccg gaaaccgtaa accgtgacaa gtagaaaccc ggttctgcct tgtcacggtt
     2401 cacggtttcc ggttcacggt tcttgtcacg gttcacggtt cacggttcac ggcttttgtt
     2461 tgttatagtg cctcttctcg caaaactctc cgcacaggat taatcgaaaa ggagtgagca
     2521 ccatgagaag acaggtgcgt ttgatcgtcg cggcgctgct gctggcctcg accgtcgcgg
     2581 cgcagggcgg catgatcgtg cccggcgaca acctcgtcgt cgagggcgtg cccgcgatac
     2641 cggcgaacat cgccgaggag gtgcggcgct acacggagtt ccgcgccgcc aacctctcga
     2701 gctggcaccc gacgaagcgc gagatgctca tctcgacgcg cttcggcgac accaaccagg
     2761 ttcaccacgt gaagatgccg ggcggcgcgc gcacgcagct cacgttcttc cccgagcgct
     2821 cgggcggcgc cgcctaccga ccgaagaccg gcgactactt cgtcttcaac aaggacatcg
     2881 gcggcaacga attcttccag ttctaccgct acgacgtggc gaccggcgac gtgacgctgc
     2941 tcaccgacgg caagtcgcgc aacacgggcg gcacttggtc gcacggcggc gagtggctcg
     3001 cctaccaatc gacgcggcgc aacggcaagg acaacgacat ctacctcgtc aaccccgcgc
     3061 agccttcgag caacaagctg ctcatcgagg tcgagggcgg cggctggggc gtctccgact
     3121 ggtcgcccga cgacaagaag ctgctggtcg gcgagttcat ctcggccaac gagtcttacg
     3181 gctacgtcgt ggacgtggcg accggcgaga agacgctgct gacgccgaag ggcggcgaga
     3241 aggtcgccta cggcggcgcg cagttcgcga aggacggcaa gggcgtctac gtcacgaccg
     3301 acaaggagtc cgagttccag cggctcgcct acatcgacct cgccaccaaa cagcacacct
     3361 acctcacaga ccatatcaag tgggacgtgg acgactttga gctctcgccg gacggctcga
     3421 ccatcgctct cgtgacgaac gaggacggca tcagccgcct ccgcctgctc gacacgaaga
     3481 cgcgcaagga gaagacgccg ccggagcttc ccgtcggcct cgtcggcgcg ctcgaatggc
     3541 acgacaacgg gcgcgacctc ggcttctctc tgtcttcggc gcgctcgacc tcggacgtgt
     3601 actcgctcga cgtgaagacg ggcaaggtcg agcgctggac gttcagcgag accggcggcc
     3661 tgaacacgga taacctctcc gacccgcagc tcgtcaagtg gaagagcttc gacgggcgcg
     3721 agatcagcgg cttcctctac ctgccgccgg cgaagttcac cggcccgcgc cccgtcatca
     3781 tcaacatcca cggcggcccc gaggggcagg cccgccccgg cttcatcggc cgcaacaact
     3841 acttcctcaa cgagcagggc gtctcgatca tctatccgaa cgtgcgcggc tcggtcggtt
     3901 tcggcaagac gttcgtcggt ctcgacaacg gcatgaagcg cgaggactcg gtgaaggaca
     3961 tcggcgcgct gctcg
//
LOCUS       DBNGOH010000815         6139 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_306249, whole genome shotgun sequence.
ACCESSION   DBNGOH010000815 DBNGOH010000000
VERSION     DBNGOH010000815.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6139)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6139)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6139
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_306249"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..672
                     /locus_tag="AC159P_15705"
     CDS             <1..672
                     /locus_tag="AC159P_15705"
                     /inference="COORDINATES: protein motif:HMM:NF017532.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HIR3696948.1"
                     /translation="HLDLRPGLLREESLFELVAHSATVPLVVQLLSPNIHLHSTTLIY
                     KRPESQDDPPFRRGWHRDIRIPRDLGHESLPLVGIKICYCLTDFQQSSCGMTLFARGT
                     HKRTEPLRIPKGEVDPEGVEVCDLRLNAGDALFFENRIFHTAAPNRSRRTSKVLMYGY
                     AYRWMKPEVYLEAPDRAQLERADPVERQLLGGYRDVDTPPWALQHWARRHHVLPEAVP
                     WTIEV"
     gene            846..1601
                     /locus_tag="AC159P_15710"
     CDS             846..1601
                     /locus_tag="AC159P_15710"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3696949.1"
                     /translation="MWNLISTRVTQFAYFDVLLGRPAWAGRKVLDFGGNVGTFLAGAG
                     DVVAHEDYWCLDLNREVVARGRRAYPRAHFVHYDRYSSQFNPAGVRHLPVPDCGVRFD
                     FVLAFSVFTHTHPREMVELVNQLRRQLLPGGALAFTFCDPAYDRSLSDPRLPAGSDVR
                     KNLEQQRRKNTPRDIDELVERASRSRWCVVIDEELYVEPGRELCLQERSGRPGESYCS
                     FFTVDYMRSLFPEAALAPPVSPEWQHCCILRNE"
     gene            1631..2722
                     /locus_tag="AC159P_15715"
     CDS             1631..2722
                     /locus_tag="AC159P_15715"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR3696950.1"
                     /translation="MNLRFHWMLPKGGEVAVDGRQTAREAARYRIESVREDSPAPRPD
                     MEGWLHFVRRAEEAGVESVLISFSRYEPDPFMVGCALGQETERLKYIIAYRSGLMQPT
                     TFVQQLNTLSGLIGGRVAVNIVAGSSASEQRGYGDFLKHDERYDRAEEFLAVCNAFWK
                     NDGEVDFDGRYYRVEKGKLHTPFTAPDRTAPEIYISGHSEKSEQLAYSQGTCWLRVID
                     TPEKLRPVVARARARGVGVCLRLALLCKPTRDEAIAAAMSLLPEDEGESTTTLKDDSQ
                     MYREGAAVGPGAHWLSPNLWAGLVPHYGPVWTTLLGTPRELADAFLAYKRIGVTEFII
                     SGWPETDEAVLFGKEILPLVREGERSTEY"
     gene            2799..3938
                     /locus_tag="AC159P_15720"
     CDS             2799..3938
                     /locus_tag="AC159P_15720"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR3696951.1"
                     /translation="MNLRFHWMMPKGGEVAMKTAQETSRVLTTKSTSPAALPDMEGWT
                     KFARAAEGAGIESVLLSFSRYEPDTILVACAVGRATTKLEFIVAYRSGLMQPTTFVQQ
                     LNTLSNLIGGRVALNVVAGSSTAEQRGYGDFLEHDERYDRAEEFLAVCRSFWRGAGEV
                     DFDGRYYQVEKGKLHTPFTAPDRTAPEIYVSGHSEQAQRLAMTRGSCYLRLIDTPEKL
                     APVVSRFRGQGIEVCLRLCVVCRPTRDEALAAAHALLPDEEIERQERAILSSSDSQTL
                     KHALATADNVGWMNRSLWAGLVPFYGSSAITLVGSPAELAEAFLAYKKIGVTQFIIAG
                     WPKLEEMLIFGRDVLPLVREAERRLDEESQETHDAWREQTEATGD"
     gene            3931..4749
                     /locus_tag="AC159P_15725"
     CDS             3931..4749
                     /locus_tag="AC159P_15725"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HIR3696952.1"
                     /translation="MIDEVSTIDEVSLNATPLAHFVARLGTARVVAEPYPHYYLENVF
                     PAAYYRELLARLPSSSVYQNLFEVTDYKLEHFRHRDQRDLKDGWTRALPAEIKGFWES
                     FDEWFRGPALAEAVLDSFAEPLRARFGAKEFWPQVSVEPQLIRHRAGYFLGPHSDLYT
                     KLVVLLIYLAPDESAGHLGTSLYRPKDAGFTCPKSLHHPFEDFVRVKTAPYRPNSMLA
                     FLRSDISFHGVEPLTDADVATASGRDLIQYVLHDRHAREAQLRAKREAAGGGYE"
     gene            4746..6104
                     /locus_tag="AC159P_15730"
     CDS             4746..6104
                     /locus_tag="AC159P_15730"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014805.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HIR3696953.1"
                     /translation="MSTPKTEGARSVVYVTRHATGESLRSARAVGQLEGVRLLCVSEE
                     PPRADAAEVFAGHARVADVHDVAQLVAAARSLDEKHGPLHQLLTAQETLLEAVAQAGA
                     ALGLGRGLSVGVVRRALDKSLLKRTLAKAGVNTARDCLITSDEDARRFAAEVGFPFVL
                     KPLSGSGGLATWRIGDAAQLERALALTRPAQERALLAEVHLRGQELCVDTITIAGEPR
                     FHSVCLYRPSIMEALEDERVLWSCVMPRDIAGERYREFVEQGLAAVRALRVGDAMTHM
                     EGFLLAGGGHCFTDATLRPAGARIGPMLGFAHDMNPYRAWARAAVDGCFDGPWERRYA
                     VGTVFLRGAGQGTIERVGGVEEVRRGVGGMIVDARLPRVGAAKSATYTGDGYVTVRHP
                     STRAVEDALRFVAETVEVAYSRAPSPAREEDAGGQWSEQLQHFTRQLNKPAWEDDSLP
                     SL"
BASE COUNT         1016 a         2127 c         2017 g          979 t
ORIGIN      
        1 cacctcgacc tgcggcccgg cctcctgcgc gaggaatcgc tcttcgaact cgtcgcgcat
       61 tcggcgaccg tcccgctcgt cgtccaactg ctcagcccca acatccacct ccactcgacg
      121 acgctcatct acaagcggcc cgagagccag gacgatccgc cttttcgccg cggctggcac
      181 cgcgacatac gcatcccgcg cgacctcggc cacgagagcc tgccgctcgt cggcatcaaa
      241 atctgctact gcctgacgga cttccaacag tcctcctgcg gcatgaccct gttcgcgcgc
      301 gggacgcaca agcgcaccga gccgctacgg attccgaagg gcgaagtcga ccccgagggc
      361 gtcgaggtgt gcgacctccg gttgaacgcg ggcgacgcgc tcttcttcga gaaccgcatc
      421 ttccacaccg ccgccccgaa ccggagccgc cgcacctcga aggtgttgat gtacggctac
      481 gcctaccgct ggatgaagcc cgaggtctac ctcgaagccc ccgaccgggc gcagctcgaa
      541 agggccgacc ccgtcgagcg ccagctcctc ggcggctacc gagacgtgga caccccgccg
      601 tgggccttgc agcactgggc gaggcgccac cacgtcctgc ccgaggccgt gccgtggaca
      661 atcgaggtct gaggattgcg gattttggat tgcggattgc ggatcaagcg ttgacgggcg
      721 tgagcgccgc catcgtcgtt aaccgtcaac tgccaatcag acaacaatcc gcaatccaaa
      781 atccgcaatc gctgttggcc cctttctcac tgtcgttccg aagttcgttc ttgcggtgct
      841 tgctcatgtg gaatctgatc tcgacacggg tcacgcagtt cgcctacttc gacgtgttgc
      901 tggggcgccc cgcgtgggcg gggcgcaagg tgctcgactt cggcggcaac gtcggcacgt
      961 tcctcgcggg cgcgggcgac gtggtcgcgc acgaggacta ctggtgcctc gacctcaacc
     1021 gcgaggtggt cgcgcgcggg cggcgcgcct acccgcgcgc gcacttcgtc cactacgacc
     1081 gctacagctc gcagttcaac cccgcgggcg tccgccacct gcccgtgccc gactgcggcg
     1141 tccggttcga cttcgtcctc gccttctccg tcttcacgca cacgcacccg cgcgagatgg
     1201 tcgagctggt caatcaactg cgccggcagc tcctgccggg cggcgccctc gcgttcacgt
     1261 tctgcgaccc ggcctacgac cgctcgctct ccgacccgcg gctgcccgcc ggctccgacg
     1321 tgcggaagaa ccttgagcag cagcgccgga agaacactcc gcgcgacatc gacgagctgg
     1381 tcgagcgcgc gagccgttcg cgctggtgtg tcgtcatcga cgaggagcta tacgtcgagc
     1441 cgggccgcga gctatgccta caggagcgca gcggcaggcc gggcgagtcc tactgctcct
     1501 tcttcacggt ggactacatg cgctcgctct tccccgaagc ggcgctcgcg ccgccggtca
     1561 gccccgaatg gcagcactgc tgcatcctcc gcaacgagtg agcgcgttcg acccgaaagc
     1621 tgtaagggcc atgaatctca gattccactg gatgctcccg aagggcggcg aggtcgcggt
     1681 cgatggtcgg cagacggcgc gcgaggcggc gcgctaccgc atcgagtccg tccgcgaaga
     1741 ctcgcccgcc ccgcgcccag acatggaggg ctggctccac ttcgtgcgcc gcgccgaaga
     1801 ggcgggcgtc gagtccgtgc tcatctcctt cagccgttat gaacccgacc ccttcatggt
     1861 cggttgcgcg ctcgggcagg agaccgaaag gctcaagtac atcatcgcct accgctccgg
     1921 cctgatgcag ccgacgacct tcgtccagca gctcaacacg ctgtcgggtt tgatcggcgg
     1981 gcgcgtcgcc gtcaacatcg tcgccggaag ctccgcctcc gagcagcgcg gctacggcga
     2041 cttcctcaag cacgacgagc gctacgaccg cgccgaagag ttcctggcgg tctgcaatgc
     2101 cttctggaag aacgacggcg aggtggattt cgacggcagg tattaccgtg tcgagaaggg
     2161 gaagctgcac acgccgttca cggcgcccga ccggacggcg cccgaaatct acatctccgg
     2221 ccactccgaa aagtcggagc aactggccta ctcgcaaggc acttgctggc tgcgcgtcat
     2281 cgacacgccg gagaagctgc ggcccgtcgt ggcgcgtgcg cgcgcgcgcg gcgtcggcgt
     2341 ctgcctgcgg ctcgcgctgt tgtgcaagcc gacgagggac gaggcaatcg ccgccgccat
     2401 gtctctgctg ccggaagacg agggcgagag cacgacgacg cttaaggacg attcgcagat
     2461 gtaccgcgag ggcgcggcgg tcgggccggg cgcgcactgg ctcagcccta atctctgggc
     2521 cgggctggtg ccgcactacg gccccgtctg gacgacgctg ctcggcacgc cgcgcgagct
     2581 ggccgacgcc ttcctcgcct acaagcgcat cggcgtcacc gagttcatca tctcgggctg
     2641 gcccgagacg gacgaggccg tcctgttcgg caaggaaatt ctcccgctcg tccgcgaagg
     2701 agaaagaagt actgagtact gagtgctgag tactgaggga aaagcttttt gtcttcaact
     2761 cagtactcag cactcagtac tcagtactca atcagactat gaacctcaga ttccactgga
     2821 tgatgccgaa gggcggcgag gtcgccatga agacggcgca ggagacttcg cgcgtgctga
     2881 cgacgaagag cacgtcgccc gccgcgctgc cggacatgga gggctggacg aagttcgcgc
     2941 gcgccgccga gggcgcgggc atcgagtcgg tgctgctctc cttcagccgc tacgagccgg
     3001 acaccatcct cgttgcctgc gccgtcggtc gcgccaccac gaaactcgaa ttcatcgtcg
     3061 cttaccgctc ggggctgatg cagccgacga ccttcgtcca gcagctcaac acgctgtcga
     3121 atttgatcgg cgggcgcgtc gctttgaacg tcgtcgccgg aagctcgacc gccgagcagc
     3181 gcggctacgg cgacttcctc gaacacgacg agcgctacga ccgcgcagag gagttcctcg
     3241 ccgtctgccg ctcgttctgg cgcggcgcgg gcgaggtgga cttcgacggc aggtactatc
     3301 aggtcgagaa agggaagctg cacacgccgt tcacggcgcc cgaccggacg gcgcccgaaa
     3361 tctatgtctc cggccattcg gagcaggcgc agcgcctcgc catgacgcgc ggctcctgct
     3421 acctgcgcct gatcgacacg ccggagaagc tggcgccggt ggtgagccgt ttccgcgggc
     3481 agggcatcga ggtctgtctc aggctgtgcg tggtctgtag accgacgcgc gacgaagcgc
     3541 tcgccgccgc gcacgcgctg ctgcccgacg aagagatcga aaggcaggag cgcgccatcc
     3601 tgtcgagcag cgattcgcag acgctcaagc acgcgctggc caccgccgac aacgtcggct
     3661 ggatgaaccg ttcgctgtgg gcggggctgg tgccgttcta cggctcgtcc gccatcacgc
     3721 tggtcggctc gcccgcggag ctggccgagg ctttcctcgc gtacaaaaag atcggcgtca
     3781 cgcagttcat catcgcgggc tggccgaagc tcgaagagat gctcatcttc gggcgcgatg
     3841 ttctgccgct cgtccgcgag gcggagcgac ggctcgacga agaatcgcaa gagacgcacg
     3901 atgcttggcg agaacagacg gaggccaccg gtgattgacg aagtgagcac gattgacgaa
     3961 gtgagcttga acgcgacccc tctcgcgcac ttcgtcgcgc ggctcggcac ggcccgcgtc
     4021 gtcgcggagc cttacccgca ctactacctc gaaaacgtct tccccgccgc ttactaccgc
     4081 gagctgctgg cgcgcctgcc ctcaagctcc gtctaccaga acctgttcga agtcacggac
     4141 tacaagctcg aacacttccg ccaccgcgac cagcgcgact tgaaggacgg gtggacgcgc
     4201 gcgctgcccg cggagatcaa gggcttctgg gagagcttcg acgaatggtt ccgtggcccc
     4261 gcgctggccg aggccgtgct cgactcgttc gccgaacctt tgcgcgcgcg gttcggcgcg
     4321 aaagagtttt ggccgcaggt ctcggtcgaa ccgcagctca tccgccaccg cgcgggttat
     4381 tttctcgggc cacactcgga cttgtacacg aagctcgtcg tgctgctcat ctacttggcg
     4441 ccggacgaga gcgcgggcca cctcggcacg tcgctctacc gcccgaagga cgcgggcttc
     4501 acctgcccga agagcctcca ccacccgttc gaggatttcg tccgcgtgaa aaccgcgccc
     4561 tacaggccga actcgatgct cgccttcctg cgctcggaca tctccttcca cggcgtcgag
     4621 ccgctgaccg acgcggacgt ggccacggcc tcgggccgcg acctgattca gtatgtgctc
     4681 cacgaccggc acgcgcgcga ggcgcagctc cgcgcgaagc gcgaagccgc gggcgggggc
     4741 tacgagtgag cacgccgaag acagagggag cgcggagcgt cgtctacgtc acacgccacg
     4801 cgacgggcga gagcctgcgc tcggcacgcg ccgtcgggca gctcgaaggc gtccgcctgc
     4861 tctgcgtctc ggaagagccg ccgcgcgccg acgcggcgga agtcttcgcg gggcacgcgc
     4921 gcgtcgccga cgtgcacgac gtagcgcaac tcgtcgccgc cgcgcgctcg ctcgacgaga
     4981 agcacggccc gcttcatcaa ctcctgacgg cgcaggagac gctgctcgaa gcggtggcgc
     5041 aggctggcgc agcgctcggc ctcgggcgcg gcctgagcgt gggagtcgtc aggcgagcgc
     5101 tcgacaagtc gctcctgaaa cggacgctcg caaaggccgg cgtcaacacg gcgcgcgatt
     5161 gtttgataac ctccgacgaa gacgcgcggc ggttcgccgc ggaggtcggc tttcccttcg
     5221 tgctgaaacc cttgagcggg agcggcggcc tcgccacgtg gcgcatcggc gacgccgcgc
     5281 agctcgaacg cgcgctcgcg ttgacacgtc cggcgcagga gcgcgccctg ctcgccgaag
     5341 tccacctgcg cggtcaggag ttgtgcgtgg acacgattac catcgcgggc gagccgcgct
     5401 tccactcggt ctgcctttac cgcccgtcga tcatggaggc tttggaagac gagcgcgtcc
     5461 tctggtcttg cgtgatgccg cgcgacatcg cgggcgagcg ttatcgtgag ttcgtcgagc
     5521 agggtctcgc ggcggtgcgc gcgctccggg tcggcgacgc gatgacgcac atggaaggct
     5581 tcctgctcgc aggcggcggg cactgcttca cggacgcgac gctgcggccc gcgggcgcgc
     5641 gcatcggccc gatgctcggc ttcgcccacg acatgaaccc gtatcgggcg tgggcgcggg
     5701 ccgccgtgga cggatgtttt gacgggccgt gggagcgccg gtatgctgtc ggcaccgtct
     5761 tcctgcgcgg cgcagggcaa ggcacgatcg agcgcgtcgg cggcgtcgaa gaggtcaggc
     5821 gcggcgtcgg cgggatgatc gttgacgcgc gcctgccgcg cgtcggggcc gcgaagtcgg
     5881 ccacctacac cggcgacggc tacgtcaccg tccgccaccc ctcgacgcgc gcggtcgagg
     5941 acgcgctccg cttcgtcgcc gagaccgtcg aggtcgctta cagccgcgcg ccgtcgcccg
     6001 cgcgcgaaga agacgcgggc ggccagtggt cggaacagct ccagcacttc acacggcaac
     6061 tgaacaaacc ggcgtgggag gacgactcgc tgccgagcct ttaacaatca agtactgagg
     6121 actgaggact gagtaaaag
//
LOCUS       DBNGOH010000816         8461 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1773330, whole genome shotgun sequence.
ACCESSION   DBNGOH010000816 DBNGOH010000000
VERSION     DBNGOH010000816.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8461)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8461)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8461
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1773330"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(204..392)
                     /locus_tag="AC159P_15735"
     CDS             complement(204..392)
                     /locus_tag="AC159P_15735"
                     /inference="COORDINATES: protein motif:HMM:TIGR01764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR3696954.1"
                     /translation="MDYLTTKEVAARLGVKIRMVQLLVQTGRLPATKFGRDYMIKPED
                     LKLVENRKVGRPATKKKS"
     gene            complement(568..651)
                     /locus_tag="AC159P_15740"
     tRNA            complement(568..651)
                     /locus_tag="AC159P_15740"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(615..617),aa:Leu,seq:caa)
     gene            886..2037
                     /locus_tag="AC159P_15745"
     CDS             886..2037
                     /locus_tag="AC159P_15745"
                     /inference="COORDINATES: protein motif:HMM:NF024878.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HIR3696955.1"
                     /translation="MSGTLGRRSRILIADDERDIREVLCDLLGGAYDCTAVGTAEEAL
                     ARLSAGRFDLVISDIMMGGMSGLALAPRVREMAPDTVVIVISGAQTIESAVEALRGGA
                     FDYMMKPFDLGHVEAAVSRALEHQSLRAAKRRYEIFLEEMVEQRTAELDRAVDSLEDA
                     YRVTLRALTAALAMRDHETHGHSERVVSFSLRLGRELGLGRAEMRALEFGSLLHDIGK
                     IGVPDAILRKPGKLDEAEWRKMREHPLNGQKILRGVGFLEGAARVVAEHHERWDGSGY
                     PAGLRGEQIDLNARIFAVADAFDAMTSDRVYRRGRGYEEAAAELEAFSGRQFDPQVIE
                     AFRRVPREEWAEMRRRSLEPRAETEIGAGLHDAATAALFITSRAASLIN"
     gene            complement(2219..3367)
                     /locus_tag="AC159P_15750"
     CDS             complement(2219..3367)
                     /locus_tag="AC159P_15750"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HIR3696956.1"
                     /translation="MLSRRSYLSLVAFAFAFMLAAPFVLAQSGVRPKPSPTPEEAPDE
                     EKIFTEEVRVPVFAFDEQGRFDPRLEVDDVLVNEDGVPQQVKSVRRLPASVLILMGTG
                     GEVSPFMRTNTTRAIAMNVLKTLREGDRIAALSFSGKTEVLHGWTEDKIQVARALKSK
                     LISGPGSDLSRAIVRSVELLREQPVGNRHLVLITDAVDTASSAEYSEATRRLIAAQTT
                     LHVISYTEVGKREMRKASKPKEAPGMTQSRADIATVGIDPTRPPGSRVGGGINPSSVN
                     SGIRLDPELRRRRKAYERDMKRGEGRMKTLAEETGGRILLAGTTEEMVAQGEEVAREI
                     DAQYVVTYQPKRPLRTASATEYRRIHIGARRIGLTLRARRGYVVGTMR"
     gene            complement(3469..4482)
                     /gene="pta"
                     /locus_tag="AC159P_15755"
     CDS             complement(3469..4482)
                     /gene="pta"
                     /locus_tag="AC159P_15755"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - pyruvate decarboxylation to
                     acetyl-CoA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HIR3696957.1"
                     /translation="MSILQNIRRRAAADPQHIVLPEGEDPRTVVAAAMCARDRVARVT
                     VLGDEEKIRAAAREASAGDLGGVQLIDHRRAPDFEKVAALYHELRRAKGLMPDEARLA
                     VRDPLYYGNLLVREGKADGSVAGATNTTAHTVRAALHCIGIRAGFKIVSSFFVMVMPR
                     ADIGADGAMIYADCGVVIEPTVPELAEIAIASADSCRALLETEPRVAMLSFSTKGSAA
                     HRLVDKVVEATRTVRARQPDLDIDGELQADAALVPQVGESKAPGSTVAGRANVLVFPD
                     LQAGNIAYKLTERLAGATAIGPILQGLDKPANDLSRGCKAEDIVDAVAITAVQAQARK
                     AAR"
     gene            complement(4594..5163)
                     /locus_tag="AC159P_15760"
     CDS             complement(4594..5163)
                     /locus_tag="AC159P_15760"
                     /inference="COORDINATES: protein motif:HMM:NF021577.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DICT sensory domain-containing protein"
                     /protein_id="HIR3696958.1"
                     /translation="MKDFSMYDCALKAAGASFEDLGTVPYVSRRDFDERPTFGFRARV
                     PCLEYISLLIENALLLRTHRAGRLYAGFEKLSRMEGVVDRYMRIADISERVYVFGEPD
                     WCPPRHPNMRVIKLPEGTRLAREWFVIADSPTLRCALVGVDEDGLSTPVLEERTFRAF
                     KTCNPAILARLASAAEGLIDDALATSGCR"
     gene            complement(5524..6255)
                     /locus_tag="AC159P_15765"
     CDS             complement(5524..6255)
                     /locus_tag="AC159P_15765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3696959.1"
                     /translation="MRTLDDKAVTSLYCVRAGRIVPLAGGFCRACEGPAREDDGRHQR
                     VTPGLLKRLGYVTTRLPDAAARGAAKRRVLCIDNRASRNLAVFLLKRAGFEVSTADSV
                     MSATESARGAGFDLYLVNHKLLHGAGGAELCDRLRAATPHSPILSYSTVTYPFRRREA
                     GRVRGGPAEPVSVTEVAGAVSEALGGRPPARPGPAPRATGDARGGGLSTAAKVLAGAA
                     GLAAAALLLGALAQARRGAPPPAGL"
     gene            complement(6556..7269)
                     /locus_tag="AC159P_15770"
     CDS             complement(6556..7269)
                     /locus_tag="AC159P_15770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696960.1"
                     /translation="MTRPGGAGLQFWRDQIESCGADQECRKVRRVNVSAAFFLSIEFQ
                     ETGFLAYRVHKAAYGDLPARPVPVQLREFLADARAVGEGVEVGVGDWQARLEANKASF
                     AAEFVTRGQFTSLYPPAMTPAEFVDALNANAGGALSPPASDALVNRLERRNRDAGASP
                     AGGGGGRDLRSAETNKAFVLMQYFGYLRRNPDDPQDTNFDGYFFWLDKLNRFDGNFVE
                     AEMVKAFISSIEYRQRFGQ"
     gene            complement(7385..>8461)
                     /locus_tag="AC159P_15775"
     CDS             complement(7385..>8461)
                     /locus_tag="AC159P_15775"
                     /inference="COORDINATES: protein motif:HMM:NF014874.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate ligase domain-containing protein"
                     /protein_id="HIR3696961.1"
                     /translation="STGAIPFAAACTDPCDGRSQGTPAMFDSLAYRNDAAIVFRRLIR
                     SLPTRRGVVGVATCDKGLPAMMMALAGIHDLPSVLVPGNGCLVAGWDSSSVREVVVEM
                     GDRLHTRVETFGLSEDARWQARDVDYTSGALTRFRVFRAGREWGEFETPLIGDFNVRN
                     CLAAIVAADAWGVSREAVRDALATFQSVKRRMQVRGTAGGVTVIDDFAHHPTAVRETL
                     QALRAKYTDGRLVAVFEPRSATSRLAVFQDEYRDAFGRADYVVISTVFHRERGSSYGQ
                     LLDTDALVRDIAARGTPAHCLDGADPIVTHLAPHLRPGDTVAIMSNGGFGGIHEKLLD
                     ALAESGRQAAVGGQQETRGADGKR"
BASE COUNT         1414 a         2647 c         2979 g         1421 t
ORIGIN      
        1 aggctgatat actcgcgcag gcgagtggtc agagagtgtc gagtcatcgc aaaccgatgc
       61 tctcacgagt cagtgactca tgaccactcg cttacttttt caaatgtgtc cagacatcag
      121 ctgctcaaat caaattgcaa atttcaccaa caacgccgcc cccaccctta gacaacttaa
      181 gcgactcttg agcaaatcaa gcatcaagat ttcttcttag tagctggcct accaactttc
      241 ctattttcca ccagcttcaa atcctccggc ttaatcatgt agtctcggcc aaatttagtt
      301 gccggaagcc tgccggtctg gacaagtaac tgaaccattc tgatctttac gcccagtctt
      361 gcagctactt ctttggtagt taaatagtcc ataagaagct tataaaataa atgagaatac
      421 tgcgtcaaca cgcaatattt ctattgataa atactgcgtt taggcgtaat attgggtcag
      481 tgtgttgctg agtgtgttaa cgctcagctt tgaaaattaa taactcggaa gggcagtaga
      541 aaagttgtag ttttctaagg aaagtgatgc cggaggtcgg actcgaaccg acatggagtt
      601 gcccccgcga gattttgagt ctcgtgcgta taccgatttc gccactccgg cgtggtgaga
      661 ggcatgatag ggtttggcgc gggcgggcgt caaccgcggg gaaggacaga gggcaagtcc
      721 ttgcgggcgt ggggcgcggg ggtggtgtaa aaaatgttgt acaaatgcca aagaagctgt
      781 aatatggccc ggcctctgta gacggcacac ctcacacggg taaaattcgc cgtcacctct
      841 cccactcaca gcgagttcgt acacctaaaa tttgggggca gaagaatgtc tgggactctg
      901 ggcaggagat cgcgcattct tatcgccgac gacgagcggg acatcaggga ggtgctgtgc
      961 gacctcttgg gcggcgctta cgactgcacg gcggtcggga cggcggagga ggcgctggcg
     1021 cgtctcagcg cggggcggtt cgacctcgtc atcagcgaca tcatgatggg cgggatgtcg
     1081 gggctggcgc tggcgccgcg tgtgcgcgag atggcgcccg acacggtggt catcgtgatc
     1141 agcggcgcgc agacaatcga aagcgcggtc gaggctttgc gcggcggcgc gttcgactac
     1201 atgatgaagc cgttcgacct cggccacgtc gaggcggcgg tcagccgcgc gctcgaacac
     1261 cagagtttgc gcgcggccaa gcggcgttac gaaatctttc tggaagagat ggtcgagcag
     1321 cggacggcgg aacttgaccg cgcggtcgat tcgctcgaag acgcgtaccg cgtcacgctg
     1381 cgcgcgctga cggcggcgct tgcgatgcgc gaccacgaga cgcacggcca ctcggagcgc
     1441 gtcgtctctt tctctctgcg gctgggccgc gagctgggcc tcgggcgcgc cgagatgcgc
     1501 gcgctcgagt tcggctcgct ccttcacgac atcgggaaga tcggcgtgcc cgacgccatc
     1561 ctgcgcaagc ccggcaagct cgacgaagcg gagtggcgga agatgcgcga gcaccctctg
     1621 aacgggcaga aaatcctgcg cggcgtcggg tttctggaag gcgcggcgcg cgtcgtcgcg
     1681 gagcaccacg aacgttggga cgggtcgggc tacccggcgg gcttgcgcgg cgagcagatc
     1741 gatttgaacg cgcgcatctt cgccgtcgcc gacgccttcg acgcgatgac gagcgaccgc
     1801 gtctaccgca gggggcgcgg ctacgaagag gcggcggcgg agctggaagc cttcagcggt
     1861 cggcagttcg acccgcaggt gatcgaggcg ttccgccgcg tcccgcgcga ggagtgggcc
     1921 gagatgcgcc gccgctcgct cgagccgcgc gccgagaccg agatcggcgc cggcctgcac
     1981 gacgcggcca ccgccgcgct cttcatcacc tcgcgcgcgg cgtcgctcat caactgaagt
     2041 gagcagcagg cagcgggcag caggcagcgg gcagtgaaaa caaaagaagg gagatgcttg
     2101 ggaggtaaag tcccaagcat ctcccttctc cggctcgatg agcgaagatg cttgggaggt
     2161 aaagtcccaa gcatcttcgt gcttctccct taactgcctg ctgcccgctg cctgctgctt
     2221 accgcatcgt gccgacgacg tagccgcggc gggcgcggag ggtgaggccg atgcggcgcg
     2281 cgccgatgtg gatgcggcgg tactcggtgg cggaggcggt gcgcagcggg cgcttgggtt
     2341 gataggtgac gacgtactgc gcgtcgatct cgcgggcgac ctcctcgccc tgcgcgacca
     2401 tctcttcggt ggtgccggcg agcaggatgc gcccgccggt ctcttcggcg agcgtcttca
     2461 tgcggccttc gccgcgcttc atgtcgcgct cgtacgcctt gcggcggcgg cgcagctcgg
     2521 ggtcgaggcg gatgccggag ttgacgctcg aagggttgat gccgccgccg acgcgcgagc
     2581 cgggggggcg cgtcgggtcg atgccgacgg tggcgatgtc ggcgcggctc tgcgtcatgc
     2641 cgggcgcctc tttcggcttg ctcgccttcc tcatctcgcg cttgccgacc tcggtgtagc
     2701 tgatgacgtg gagcgtggtc tgcgcggcga tgaggcggcg cgtcgcttcg gagtattcgg
     2761 cggagctggc cgtgtccacg gcgtccgtga tgagcacgag gtgacggttg ccgacgggct
     2821 gctcgcgcag cagctcgacc gagcgcacga tggcccgcga aaggtcggag ccggggccgg
     2881 agatgagctt gctcttgagc gcgcgcgcga cctgaatctt gtcttccgtc cagccgtgca
     2941 ggacttcggt cttgccgctg aagctgaggg ccgcgatccg gtctccttcg cgcaaggtct
     3001 tgaggacgtt catggcgatg gcgcgcgtcg tgttcgtccg catgaagggg ctgacctcgc
     3061 cgcccgtgcc catgaggatg agcaccgagg cgggcaggcg ccgcacgctc ttcacctgct
     3121 gcggcacgcc gtcctcgttg acgagcacgt cgtccacttc gagccgcggg tcgaagcgcc
     3181 cctgctcgtc gaaggcgaag acggggacgc gcacctcctc ggtgaaaatc ttctcctcgt
     3241 ccggcgcctc ctcgggcgtc ggcgaaggct tcgggcgcac gccgctctgc gcgagcacga
     3301 aaggggcggc gagcatgaaa gcgaaggcga aggcgacgag agaaaggtac gaccggcgcg
     3361 aaagcataag gcgctccgtg ggctgcaaaa gttcgaatga ctgcccgttt gagatgcggc
     3421 gcgggcgcgg cgcggttcgc cgcccttcga gaatgacgcc ggctcgggtc agcgcgcggc
     3481 cttgcgcgcc tgcgcctgaa ccgccgtgat ggcgacggcg tccacgatgt cttcggcctt
     3541 gcagccgcgc gagaggtcgt tggcgggttt gtccaagcct tgcaggatgg ggccgatggc
     3601 cgtcgccccg gcgaggcgtt cggtgagctt gtaggcgatg ttgccggctt gcaggtcggg
     3661 gaagacgagg acgttggcgc ggcccgcgac ggtcgagccg ggcgccttcg actcgccgac
     3721 ttgcgggacg agcgcggcgt cggcctgtaa ctcgccgtcg atgtcgaggt cgggctggcg
     3781 ggcgcggacg gtgcgcgtcg cctcgaccac tttatcgacg agcctgtgcg cggcgctgcc
     3841 cttcgtcgag aaggagagca tggcgacgcg cggctcggtt tcgagcagcg cgcggcacga
     3901 gtcggcggag gcgatggcga tctcggccaa ctccggcacc gtcggctcga tgacgacgcc
     3961 gcagtcggcg tagatcatcg cgccgtcggc gccgatgtct gcacgcggca tgaccatcac
     4021 gaagaagctc gaaacgatct tgaagccggc gcggatgccg atgcagtgga gcgcggcgcg
     4081 cacggtgtgc gcggtcgtgt tggtcgcgcc cgcgacggag ccgtcggcct tgccttcgcg
     4141 caccagcagg ttgccgtagt agagcgggtc ccggacggcg aggcgcgcct cgtcgggcat
     4201 caagcctttg gcgcggcgca gctcgtggta gagcgcggcg actttctcga agtcgggcgc
     4261 gcgtcggtgg tcgatgagct ggacgccgcc gaggtctccg gcgctggcct cgcgcgcggc
     4321 ggcgcgaatc ttctcctcgt cgccgagcac ggtgacgcgg gccacgcggt cgcgcgcgca
     4381 catcgcggcg gcgacgaccg tgcgcgggtc ttcgccctcg ggcaggacga tgtgttgcgg
     4441 gtcggcggcg gcgcggcgtc gtatgttttg cagtatggac atagaacgca gaagtcaggg
     4501 gtcggaagtc ggaagtcgat aggccgctaa tttaacatga cgcggtcgaa gaaggcaggc
     4561 gagacggatg gagagacggg cggcgagacg atttcaacgg caacccgacg tggcgagggc
     4621 gtcgtcgatc aacccttcgg cggcggaggc gaggcgcgcg aggattgcgg ggttgcaggt
     4681 cttgaaggcg cggaaggtgc gttcttcgag gacgggggtc gagaggccgt cctcgtccac
     4741 gccgaccagc gcgcagcgca gcgtcggcga gtcggcgatg acgaaccact cgcgcgcgag
     4801 ccgcgtgccc tcgggcagtt tgatgacgcg catgttgggg tggcgcggcg ggcaccagtc
     4861 cggctcgccg aagacgtaga cgcgctcgga gatgtcggcg atgcgcatgt agcggtcaac
     4921 gacgccctcc atccgcgaga gcttctcgaa gccggcgtac agtcgtcccg cccggtgagt
     4981 gcgcaacagc agcgcgttct cgatcaggag gctgatgtac tcaagacagg ggacccgcgc
     5041 gcggaagccg aatgtcggtc gctcgtcgaa gtcccggcgt gaaacgtagg gaaccgtgcc
     5101 gaggtcttcg aacgacgcgc ccgccgcctt cagcgcgcag tcgtacatcg aaaaatcttt
     5161 catggcgcgc tcttcgtgtc ccggcgcccg ccgaagcggc tcagcggcgc cgaagcaatt
     5221 tactacgcgt ccaaagccac gccagcatta cgaatgtttc gtgaaagtgt gtgcggtggg
     5281 caacttaaca gtggaaaccg cttaatgcgt tcagccgtcg aaagcccggc gcggattgcg
     5341 gtcgagttta ccgagaccgg gattcaacgc ggagggcgca gaggcgcaga gggcaccgag
     5401 aagactttcc tttctctgtg ccctccgcgt ttatccctgc tgccgtcaaa ctcaacggca
     5461 gagtgctctg gtgaaacgtt cggtcattat cctttcatct acggcgaggg gatatttacc
     5521 gaactaaaga ccggcgggag gcggcgcgcc ccgccgcgcc tgagccagcg cacccagcag
     5581 caacgccgcc gccgccaagc ccgccgcccc ggcgagcact ttcgccgcgg tcgataaccc
     5641 tccgcctcgc gcatcccccg tcgcgcgggg cgcggggccg gggcgggcgg gcgggcgccc
     5701 gcccaatgcc tccgacaccg ccccggccac ctccgtcacg gacaccggct ccgccggacc
     5761 gccgcgaacg cgtcccgcct cacgccgccg gaaagggtat gtcacggtcg agtaggagag
     5821 gatcggcgaa tggggcgtgg ccgcacgcag cctgtcgcat aactcggcgc cccccgcccc
     5881 gtgcagcagc ttgtggttca cgagatagag gtcgaagccc gcgccgcgcg ccgattccgt
     5941 ggcggacatg accgagtcgg cggtcgagac ttcgaagccg gcccgcttga ggaggaagac
     6001 ggccaggttg cgcgaggcgc ggttgtcgat gcagaggacg cggcgcttgg ccgcgccccg
     6061 cgccgccgcg tcgggcagcc gcgtcgtcac gtaccccagc cgcttgagca gccccggcgt
     6121 cacacgctgg tgcctgccgt cgtcttcccg cgccggccct tcgcacgcgc ggcagaaccc
     6181 gccggcgagc gggacgatcc tgcccgcacg cacgcaatag agtgatgtca ccgccttgtc
     6241 gtcaagtgtt cgcataagcc ctcccgccgg atatgagttc gcaagaccgc acagcccctc
     6301 ggcaaaagca ctaacggggt gccgagactt gactcggcac cccgtctgcc ctaagtgaaa
     6361 agcaatgatg tctcgattct acgcgcgtcg ggcgacgccc gtggttgtga tcgtgttgcg
     6421 ggttggtgaa tttttgcccc cccgaaggcg caacggctta ccggatgtcg gatagtgctt
     6481 cgttccagcc gtaggcgccg gcgtgtcgct ggaaacgacg cgtccggccc tgaccttcac
     6541 gtgaacatag catcgctatt ggccgaaacg ctgacggtac tcgatcgagg agatgaacgc
     6601 cttgaccatc tcggcctcga cgaagttgcc gtcgaaccgg ttcagtttgt ccagccagaa
     6661 gaagtacccg tcgaagttgg tgtcctgcgg gtcgtccggg ttgcgccgca gatacccgaa
     6721 gtactgcatc aggacgaacg ccttgttcgt ctcggcgctc cgcaggtcgc gtcctccgcc
     6781 accgcccgca ggacttgcgc ccgcgtcacg gttccgccgt tcaaggcggt tcaccagagc
     6841 gtcgctcgcg ggcggcgaga gcgcgccgcc cgcgttcgcg ttgagcgcgt ccacgaactc
     6901 ggcgggcgtc atcgccggcg ggtaaaggct cgtgaactgt ccgcgcgtca cgaactcggc
     6961 ggcgaacgaa gccttattgg cttcgagccg cgcctgccag tcgccgacgc cgacctcgac
     7021 gccctcgccg accgcacgcg cgtccgccag aaactcgcgc agttggacgg gaacgggacg
     7081 ggccgggagg tcgccgtagg cggccttgtg cacgcggtag gccagaaagc ccgtctcctg
     7141 gaattcgatg gagaggaaga aggccgcaga gacgttgacg cgcctgacct tgcggcactc
     7201 ctggtcggcg ccgcacgact cgatctgatc cctccagaac tggaggcccg cgccgccggg
     7261 ccgtgtcaaa gatgagcggg tagccgtcgg atgtcggctg aagtggggcg cggcgggcgg
     7321 gcaatggaat cgtagcgggg cgcgaataaa gcgtgcgggc ggcaggcggt tcagggaaac
     7381 gctctcaccg cttgccgtcc gcgccccttg tttcttgctg accgccgacc gcggcctgcc
     7441 gcccgctctc cgcgagcgcg tcgaggagct tttcgtggat gccgccgaag ccgccgttgg
     7501 acatgatggc gacggtgtcg ccgggtctga ggtgcggcgc gaggtgcgtg acgatggggt
     7561 ctgcgccgtc gaggcagtgg gcgggtgtgc cgcgcgcggc gatgtcgcgg acgagcgcgt
     7621 cggtgtcgag gagttggccg taggaactgc cgcgctcgcg gtggaagacg gtgctgatga
     7681 cgacgtagtc tgcgcgcccg aaggcgtcgc ggtattcgtc ctgaaagacg gcgaggcgcg
     7741 aggtggccga gcgcggctcg aagacggcga cgagacgccc gtcggtgtac ttggcgcgca
     7801 gcgcttgcag cgtctcgcgc acggcggtcg ggtggtgcgc gaagtcgtcg atgacggtga
     7861 cgccgcccgc cgtgccgcgc acctgcatcc gccgcttgac gctctggaag gtggcgagcg
     7921 cgtctcgcac ggcctcgcgc gagacgcccc acgcgtcggc ggcgacgatg gcggcgaggc
     7981 agttgcggac gttgaagtcg ccgatgagcg gcgtctcgaa ctcgccccac tcgcggcctg
     8041 cacggaagac gcggaagcgc gtcagcgcgc ccgaagtgta atccacgtcg cgcgcctgcc
     8101 agcgcgcgtc ctcgctcagg ccgaacgtct cgacgcgcgt gtggaggcgg tctcccatct
     8161 cgacgacgac ttcgcggacg gaggagctgt cccatccggc gacgaggcac ccgttgccgg
     8221 ggacgaggac cgacggcaaa tcatggatgc ccgccagggc catcatcatc gccggcaatc
     8281 ctttgtcgca ggtcgcgacg ccgaccaccc cgcggcgagt cgggagggac cgaatcagac
     8341 gccggaagac gatcgccgca tcgttgcgat aggcgaggct atcgaacatc gcgggcgtgc
     8401 cttgcgagcg gccatcgcac ggatcggtgc acgcggcggc gaagggaatc gcgccggtgc
     8461 t
//
LOCUS       DBNGOH010000817         1625 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3788159, whole genome shotgun sequence.
ACCESSION   DBNGOH010000817 DBNGOH010000000
VERSION     DBNGOH010000817.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1625)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1625)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1625
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3788159"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..112
                     /locus_tag="AC159P_15780"
     CDS             <1..112
                     /locus_tag="AC159P_15780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HIR3696962.1"
                     /translation="AGRPGHVFNLGHGILPETPVENVIALVEAVHELSKR"
     gene            173..1252
                     /locus_tag="AC159P_15785"
     CDS             173..1252
                     /locus_tag="AC159P_15785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - protoporphyrin ferrochelatase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HIR3696963.1"
                     /translation="MTHSGLQTPDSRLQTDFDALLFVSFGGPEGVEEVMPFLGNVLRG
                     RNVPPERMREVAHHYELLGGVSPINGQNRRLIAAVEKELAEHGPRLPVYWGNRNWHPL
                     LADTLRRMKEDGVRRALAFVTSAYSSYSGCRQYREDIERARAEVGEGAPVVEKLRVFY
                     NHPGFVAPNVENLRAALEEIPEERRAASRVAFTAHSIPAAMAANCDYEKQLLETSRLV
                     AEGAGHAGWRLVFQSRSGPPTQPWLAPDICDHLRELKGEGVRDVVVAPVGFISDHMEV
                     LYDLDTEARQVADAAGLNMIRAATVGTHPSFVRMIRELILERTDAAAAPLSLGTHGPR
                     ARACPADCCLPAAMRPPAAASTNSE"
     gene            1245..>1625
                     /locus_tag="AC159P_15790"
     CDS             1245..>1625
                     /locus_tag="AC159P_15790"
                     /inference="COORDINATES: protein motif:HMM:NF013739.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR3696964.1"
                     /translation="MNEVRSSTRRVVVIGGGVSGLAAAHRLAELNAREASARGGVFAR
                     VEVLLLEASGRLGGTVRTERREGFLLEGGPDSFISEKPAALELAKRVGLADRLIGTND
                     EHRRSFVVRGGRLLPTPEGFQLLAP"
BASE COUNT          264 a          599 c          533 g          229 t
ORIGIN      
        1 ggcgggccgc cccggccacg tcttcaacct cggccacggc atcctgcccg agacgccggt
       61 cgagaacgtc atcgcgctcg tcgaagccgt ccacgagctg agcaaaagat gagtccggag
      121 tcagaacaaa aggcgggaag aattcaacgc gacgaagagc taacaaacta acatgacgca
      181 ctccggactc cagactccag actcccgact ccagaccgat ttcgacgcgc ttttattcgt
      241 ctccttcggc gggccggaag gcgtggaaga ggtgatgccc ttcctcggga acgtgctgcg
      301 cgggcgcaac gtgccgcccg agaggatgcg cgaggtggcg caccactacg agctgttggg
      361 cggcgtcagc cccatcaacg ggcagaaccg caggctcatc gccgccgtcg aaaaggaact
      421 cgccgagcac ggcccccgcc tcccagtcta ctggggcaac cgcaactggc acccgctgct
      481 cgccgacacc ctgcgccgca tgaaggagga cggcgtgcgc cgcgcgctcg cgttcgtcac
      541 ctccgcctac agttcttact cgggctgccg gcagtaccgc gaggacatcg agcgtgcgcg
      601 cgcggaggtc ggcgagggcg cgcccgtcgt cgagaagctg cgcgtcttct acaaccaccc
      661 cggcttcgtc gcgccgaatg tcgagaactt gcgcgccgcg ctcgaagaga ttcccgaaga
      721 gaggcgcgcg gcctcgcgcg tcgccttcac ggcgcacagc atccccgccg cgatggccgc
      781 gaactgcgac tacgaaaagc agctccttga gacctcgcgc ctcgtcgcag agggcgcggg
      841 ccacgcgggc tggcgcctcg tcttccagag ccgcagcggg ccgcccacgc aaccgtggct
      901 cgcgcccgac atctgcgacc acctgcgcga gctgaaaggc gagggcgtcc gcgatgtcgt
      961 cgtcgcgccc gtcggcttca tctccgacca catggaagtg ctctacgacc tcgacacgga
     1021 ggcgcggcag gtcgccgacg cggcggggct gaacatgatt cgcgccgcca cggtcggcac
     1081 gcacccttcg ttcgtccgca tgatccgcga gctgatactg gagcggacgg acgccgccgc
     1141 cgcgcccctc tcgctcggca ctcacggccc gcgcgcgcgc gcgtgccctg ccgactgttg
     1201 cctgcctgcg gccatgagac cgcccgccgc cgcttctacg aacagtgaat gaagtccgga
     1261 gcagcacgcg ccgcgtcgtc gtcatcggcg gcggcgtcag cggcctcgcc gccgcgcacc
     1321 gtctcgccga gctgaacgcg cgcgaagcgt ccgcgcgggg cggcgtcttc gcgcgcgtcg
     1381 aagtcctgct gctcgaagcc tccgggcggc tcggcggcac cgtccgcaca gagcggcgcg
     1441 aaggcttcct gctcgaaggc gggccggact ctttcatctc ggaaaagccc gcggcgttgg
     1501 agttggcgaa gcgcgtcggg cttgccgacc gtctgatcgg cacgaacgac gagcaccgcc
     1561 gcagcttcgt cgtccgcggc gggcgcctgc tgccgacgcc cgaaggcttt cagctcctcg
     1621 cgccc
//
LOCUS       DBNGOH010000818         4015 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_306440, whole genome shotgun sequence.
ACCESSION   DBNGOH010000818 DBNGOH010000000
VERSION     DBNGOH010000818.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4015)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4015)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4015
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_306440"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            144..677
                     /locus_tag="AC159P_15795"
     CDS             144..677
                     /locus_tag="AC159P_15795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696965.1"
                     /translation="MKIVDRFAGQNWLITPAALAVNEPRPQNIQGQKWLMVLSGVVFA
                     EVKGNSTSQWFRETLHLSPDILSPMNFAVSRHSIPTPPGGGTKAFQVEQWSPFAAPSS
                     MFNQNQSIDSGFAVDVWRPAPFGTGTNELTSQPLNNLFNGIQVDVAVRDTDAILHRVS
                     YNITLLGKIVFLRNIIL"
     gene            complement(864..2654)
                     /locus_tag="AC159P_15800"
     CDS             complement(864..2654)
                     /locus_tag="AC159P_15800"
                     /inference="COORDINATES: protein motif:HMM:NF036516.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M61 family metallopeptidase"
                     /protein_id="HIR3696966.1"
                     /translation="MRAKNFRLRLFVLTLVILSAPATFAQGDRLGVEYTVEVKDKAER
                     LFHITTHLRNIRQPRLALSLPVWTPGWYTIENYAKNILRFTVTDARGQRLPHRMSRKQ
                     TWDVETKGLSEIKVDFDYRADVLGLNQAKIADDFAFFTGTQLFLEPVGHRAAPSTVRF
                     RVPAGWKIVSALDETRDPHVFTATDYDALVDAPTEMGHFDVTRFEVEGKPHYFVATPA
                     GAFNAEKSRQFTAMFDKFARAQGAIFGGLPYEKYVAFYFFMPAESNAAGAVEHANSYV
                     AFAPSGEVATPDMILGTAAHEFFHLWNVKRFRPAEMWPYDYSREQETPLLWVSEGLTN
                     YYTNVSLLRSGLRRPEQFLESVSNAITNNESDEARPYISPADASTSTWLGYDTPVAFG
                     ISYYTQGQNLGALLDLSIRHDTAGRASLDDVMRALYSDFYLRGRGFTTDDLLAVIKRV
                     AGRDYTDFFRRHVFGVEVPPYDRILGHAGYRLEKSARKTPHLGILNARPAQGGLELVR
                     LEPNSPATRAGLAPGDVILKIDGADFRAFSLGDSAGKTVALRYKRAGEEKETGLDIGT
                     LDETSYRIAELPNPSAEQLKIRAGWMNAGK"
     gene            complement(2859..3326)
                     /locus_tag="AC159P_15805"
     CDS             complement(2859..3326)
                     /locus_tag="AC159P_15805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017314580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696967.1"
                     /translation="MSATHIAPPHYETAEEVETLVRRFESCTLPFAEWTHPAHLTVAL
                     WYLLRHEWPEAVSLVREGIRRYNHAHGVPTTRDRGYHVTLTIFWKRHVRAFLADSFNE
                     GRSLVRLANELVEGADKHLPLRHYTRELLFSWEARLGWVEPDLEPLKAVNGKP"
     gene            complement(3406..3480)
                     /locus_tag="AC159P_15810"
     tRNA            complement(3406..3480)
                     /locus_tag="AC159P_15810"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3445..3447),aa:Gln,seq:ctg)
     gene            complement(3493..3563)
                     /locus_tag="AC159P_15815"
     tRNA            complement(3493..3563)
                     /locus_tag="AC159P_15815"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3529..3531),aa:Ile,seq:gat)
     gene            complement(3570..3645)
                     /locus_tag="AC159P_15820"
     tRNA            complement(3570..3645)
                     /locus_tag="AC159P_15820"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3610..3612),aa:Lys,seq:ttt)
     gene            complement(3662..3734)
                     /locus_tag="AC159P_15825"
     tRNA            complement(3662..3734)
                     /locus_tag="AC159P_15825"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3699..3701),aa:Asn,seq:gtt)
     gene            complement(3816..3891)
                     /locus_tag="AC159P_15830"
     tRNA            complement(3816..3891)
                     /locus_tag="AC159P_15830"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3856..3858),aa:Met,seq:cat)
     gene            complement(3902..3973)
                     /locus_tag="AC159P_15835"
     tRNA            complement(3902..3973)
                     /locus_tag="AC159P_15835"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3938..3940),aa:Ala,seq:tgc)
BASE COUNT          719 a         1170 c         1381 g          745 t
ORIGIN      
        1 tcgctcaact gccggccacc atattttcag gcttcgaccg gctgggacac gtttctctca
       61 ggcgcgcata tatttgtgag accgcttccg gtttttggtc gggggcggtg ccgaatcact
      121 tttgacgcgt ggggaggaac actatgaaga tcgttgacag attcgcgggc cagaactggc
      181 tcatcacgcc tgctgcttta gcggtgaatg aacccaggcc ccaaaacata caggggcaga
      241 agtggctgat ggtgctttcc ggcgtcgttt tcgccgaagt caaaggcaac agcacatcgc
      301 agtggtttcg tgaaactctg cacctcagcc cggacatcct cagccccatg aatttcgcgg
      361 tcagcaggca ttccataccg acaccgcccg gcggcgggac aaaggcgttt caggtcgaac
      421 agtggtcgcc cttcgcggcc ccgagttcga tgttcaacca gaaccagtcc atcgactccg
      481 ggttcgcggt cgatgtctgg cgacctgcac ctttcggcac cgggacgaac gagctgacaa
      541 gtcaaccgct gaacaacctt ttcaacggca ttcaggtgga cgtggccgtg cgcgacacgg
      601 acgccatcct tcaccgcgtc agctacaaca tcactctgct cgggaagatc gtctttctca
      661 ggaacatcat cctttgaagc tcgtcgcgtg tcgccagatg ttccgcttca ggcgagaggc
      721 ggtcggcgcg ccccgcccgg caagccgtcc gggcggggcg cgggacggcg tgatgctcaa
      781 gctgtggcct tcggtctcgg ctcatgcgtc gcgcccgcgc gtttcccgcg tccgttccgc
      841 ctcgtcgtag acccgttcga ggctcacttg cctgcgttca tccaacccgc gcgaatcttc
      901 agttgttcgg ctgaagggtt cggcagctcc gcgatgcggt aggaggtctc gtcgagggtg
      961 ccgatgtcga gtccggtctc cttttcttcg cccgcgcgtt tgtagcggag ggcgacggtc
     1021 ttgcccgcgc tgtcgcccaa gctgaaggcg cggaagtcgg cgccgtcgat cttcaagatc
     1081 acgtcgccgg gggcgagtcc ggcgcgggtg gcgggggagt tgggttcgag gcggacgagt
     1141 tcgaggccgc cctgcgcggg gcgcgcgttg aggatgccga ggtgcggggt tttgcgcgcc
     1201 gacttctcaa gcctgtagcc cgcgtggccg agtatccggt cgtagggcgg cacttcgacg
     1261 ccgaagacgt ggcggcggaa gaagtctgtg tagtcgcggc ccgcgacgcg tttgatgacc
     1321 gcgagcaggt cgtccgtggt gaagccgcgc ccgcgcaggt agaaatcact gtagagcgcg
     1381 cgcatcacgt cgtcgagcga agcgcggccc gcggtgtcgt ggcggatcga gaggtcgagc
     1441 agcgcgccga ggttctgccc ctgcgtgtag taggagatgc cgaaagcgac gggcgtgtcg
     1501 tagccgagcc acgtcgaggt cgaggcgtcg gcgggcgaga tgtaggggcg cgcctcgtcc
     1561 gactcgttgt tggtgatggc gttcgagacg gactccagaa actgttccgg cctgcgcagg
     1621 cccgagcgca ggagcgagac gttggtgtag tagttcgtga ggccctcgga gacccagagg
     1681 agcggcgtct cctgctcgcg cgagtagtcg tagggccaca tctctgcggg gcggaagcgc
     1741 ttgacgttcc agaggtggaa gaactcgtgc gcggcggtgc cgaggatcat gtcgggcgtg
     1801 gcgacttcgc cggacggggc gaaggcgacg taggagttgg cgtgctcgac cgcgcccgcg
     1861 gcgttcgact cagccggcat gaagaagtag aaggcgacgt acttctcgta aggcagcccg
     1921 ccgaagatgg cgccctgcgc ccgcgcgaac ttgtcgaaca tcgcggtgaa ctgccgcgac
     1981 ttctcggcgt tgaaggcgcc cgccggggtc gcgacgaagt agtgcggctt gccttcgacc
     2041 tcgaagcgcg tcacgtcgaa gtggcccatc tcggtcggcg cgtccacgag cgcgtcgtag
     2101 tcggtcgcgg tgaagacgtg cgggtctctg gtctcgtcga gggccgagac gatcttccag
     2161 cccgcgggga cgcggaagcg gacggtgctc ggcgcggcgc ggtggccgac gggttcgagg
     2221 aagagctgcg tgccggtgaa gaaggcgaag tcgtcggcga tcttcgcttg attgaggccg
     2281 agcacgtcgg cgcggtagtc gaagtcaacc ttgatctcgc tcagcccctt cgtctccacg
     2341 tcccacgtct gcttgcgcga catcctgtgc gggagcctct ggccgcgcgc gtccgtgacg
     2401 gtgaagcgca ggatgttctt cgcgtagttc tcgatggtgt accagccggg agtccagacc
     2461 gggagcgaca gcgcgaggcg cggctgccgt atgttccgca agtgcgtggt gatgtggaag
     2521 agccgctctg ccttgtcctt gacctcgacc gtgtactcga cgccgagccg gtcgccctgc
     2581 gcgaacgtcg ccggggccga gaggatgacg agggtgagga cgaaaagccg gagcctgaag
     2641 tttttcgcgc gcatgttttt cttccgtgaa tctcgtgtgg acgtggacga gctaggtgcg
     2701 cgcacgtgcg gcgtccacag gggcgccggg cgtgcgcgtc ggggagagca tgactgttga
     2761 tttcttacag gttggggcgg aaggtgtcaa cgcggaaaag ccgtcaaccg tgagccgtca
     2821 accgtgacaa gaagaaacgg gttcttcctt gccacggttt acggtttacc gttcacggct
     2881 tttaacggct ccaggtcagg ctcgacccag ccgaggcgcg cctcccagga gaagagcagc
     2941 tcgcgtgtgt agtggcggag cgggaggtgc ttgtcggcgc cctcgaccag ttcgttggcg
     3001 agccggacga gcgagcggcc ctcgttgaag ctgtcggcga ggaaggcgcg gacgtggcgc
     3061 ttccagaaga tcgtcagcgt cacgtggtag ccgcggtcgc gcgtcgtcgg gacgccgtgc
     3121 gcgtggttgt agcgccggat gccctcgcgc accagcgaga cggcctcggg ccattcgtgg
     3181 cgcagcagat accagagcgc gaccgtcagg tgcgcggggt gcgtccactc cgcgaagggg
     3241 agcgtgcatg actcgaagcg gcgcacgagc gtctccacct cttcggcggt ctcgtagtgc
     3301 ggcggtgcga tgtgcgtggc ggacatgtga acgacctctc gcggaatctc gatcgaagcg
     3361 cccggtgcag tcgcaggtga gaaaagggaa agagtggaag aaggttggct gcggagaaag
     3421 gattcgaacc tctacgacca gtttcagaga ctggcagcct accggttagc tgactccgca
     3481 aggtgaaaac aatcgcggcg ggtggagtcg aaccaccgta tctcgattat cggtcgaacg
     3541 ctctgccgtt gagctacgcc gcgcgaggct ggttgacccg acgaggctcg aactcgcacc
     3601 tcgcagatta aaagtctgcc gcactgccac tcgtgctccg ggtcagcaag ggccacgaaa
     3661 actccggcgg agggattcga acccccgatg actgcattaa cagtgcagcg ccttaccagc
     3721 ttggcgacac cggaagaaaa gaaaaggact gaggactgag gactgggctt gaagacagat
     3781 tgtttttaca cagtcctcag tcattttcaa aagcatcgcg ggggcgggaa tcgaacccgc
     3841 ctttaaaaac ggcttatgag accgccaagc ccaccagggc ttctccccac agcgaaacga
     3901 atagaacggg agggagtcga accctcacag aaagtttgca ggactttcat gctgccattt
     3961 acatcaccgt cccttactca gtactgagtg ctgaggactg aggactgagt aaaag
//
LOCUS       DBNGOH010000819         1792 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1224573, whole genome shotgun sequence.
ACCESSION   DBNGOH010000819 DBNGOH010000000
VERSION     DBNGOH010000819.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1792)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1792)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1792
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1224573"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            44..496
                     /gene="fabZ"
                     /locus_tag="AC159P_15840"
     CDS             44..496
                     /gene="fabZ"
                     /locus_tag="AC159P_15840"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HIR3696968.1"
                     /translation="METVLDTLAIQRILPHRYPFLLVDRIVEITEGERIVGVKQVTIN
                     EPFFQGHFPGAPVMPGVLIVEAMAQVGAVYALRRIEDRERKLVLFSGIDNARFRRPVV
                     PGDTLVLTVTPLRVGGRVQRMHGEAHVEGQLAAEADIMSVVADRGAVQ"
     gene            594..1373
                     /gene="lpxA"
                     /locus_tag="AC159P_15845"
     CDS             594..1373
                     /gene="lpxA"
                     /locus_tag="AC159P_15845"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020712817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HIR3696969.1"
                     /translation="MSVTIHPTAVVSPRAEIGRGSVVGPYTVVGEEVRLGERVRLDSH
                     CVVEGRTEIGDDTHVFPFVSLGLAPQDLKYRGEPAETRIGRRNQLREFVTVHRGTEGG
                     GMLTSIGDDCLVMAQAHVAHDCHIGDGVIMANAATLAGHVTVEDGANVGAYSGVHQFC
                     RVGREAYVGGYSVVVKDALPFALTVGNHARCYGLNTVGMKRRHYPQETIDALHHAFRL
                     LLASRLNTTQALAKIREEIQGSDEVEELVRFIEASERGVVK"
     gene            1401..>1792
                     /locus_tag="AC159P_15850"
     CDS             1401..>1792
                     /locus_tag="AC159P_15850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696970.1"
                     /translation="MLKIMSCGVFMLMLSSVALVTGQEPDVLIYKGKTYKLFSNPLES
                     FYKNENDRPAFKVAPNVLSSTGNWRGYVATWEIRNDLLYLTNIDSWICNFQEYMAKKC
                     KKADLEGLFGEKYVDGRVLAGWFSGELLV"
BASE COUNT          328 a          594 c          535 g          335 t
ORIGIN      
        1 ttttactcag cactcagcac tcagcactca gcactatatt tttatggaaa cggttctcga
       61 cacgctcgcc atccagcgca tcctgccgca ccgctacccg ttcctgctcg ttgaccgcat
      121 cgtcgagatc accgagggcg agcgcatcgt cggcgtcaag caggtgacga tcaacgagcc
      181 gttcttccag ggccacttcc ccggcgcgcc cgtcatgccc ggcgtcctga tcgtcgaggc
      241 gatggcgcag gtcggcgccg tctacgcgct gcgccggatc gaagaccgcg agcgcaagct
      301 cgtcctcttc agcggcatcg acaacgcgcg cttccgccgc cccgtagtcc ccggcgacac
      361 gctcgtcctg accgtcacgc ccctgcgcgt cgggggccgc gtccagcgca tgcacggcga
      421 ggcccacgtc gaaggccagc tcgccgccga ggccgacatc atgagcgtcg tcgccgaccg
      481 cggcgcggtg caatgaaggg ggaagagggg aagaggggaa aaggcgaaaa gggttttggt
      541 ctttctccct tttcccctct tcccctttta accttttccc ctttgagttt ttgatgtccg
      601 tcacgattca tcccaccgcc gtcgtcagcc cgcgcgccga gatcgggcgc ggctcggtcg
      661 tcggcccgta cacggtcgtc ggcgaagagg tgcggctcgg cgagcgcgtg cggctcgact
      721 cgcactgcgt cgtcgagggg cgcaccgaga tcggcgacga cacgcacgtc ttccccttcg
      781 tctcgctggg cctcgcgccg caggacttga agtaccgcgg cgagccggcg gagacgcgca
      841 tcggcaggcg caaccaactg cgcgagttcg tcaccgtcca ccgcggcacg gagggcggcg
      901 ggatgctcac ctccatcggc gacgactgcc tcgtcatggc gcaggcccac gtcgcgcacg
      961 actgccacat cggcgacggc gtcatcatgg ccaacgccgc cacgctcgcc ggccacgtca
     1021 ccgtggagga cggcgcgaac gtcggcgcct actcgggcgt ccaccagttc tgccgcgtcg
     1081 ggcgcgaggc ctacgtcggc ggctactcgg tcgtcgtcaa ggacgcgctc cccttcgccc
     1141 tcaccgtcgg caaccacgcc aggtgttacg gcctcaacac cgtcggcatg aagcgccgcc
     1201 actacccgca ggagaccatc gacgcgctgc accacgcctt ccgcctgctg ctcgcttcca
     1261 ggctcaacac cacgcaggcg ctcgccaaga ttcgcgaaga gattcaaggc tcggacgagg
     1321 tcgaggaact cgtccgcttc atcgaagcgt cggagcgcgg cgtcgtcaaa tgaaatttcc
     1381 tctcaccatc ggagggcgca atgcttaaga ttatgtcatg cggtgtcttc atgctgatgc
     1441 tctcatcagt tgcgcttgtc acgggccaag agccggacgt gctcatctat aaaggtaaaa
     1501 cctacaaact gttttctaac ccgctggagt cgttctacaa aaacgagaat gaccgccccg
     1561 ctttcaaagt cgcgccgaac gtcctttcga gtaccggcaa ttggcgcggg tacgttgcga
     1621 cttgggaaat cagaaatgac ttgttgtatc tgacgaacat agattcctgg atttgtaatt
     1681 tccaagagta catggctaag aagtgtaaga aagcagacct agaagggtta ttcggcgaga
     1741 agtatgttga cggcagggtg ctggccggtt ggttttcggg agaactgctc gt
//
LOCUS       DBNGOH010000820         4210 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4705107, whole genome shotgun sequence.
ACCESSION   DBNGOH010000820 DBNGOH010000000
VERSION     DBNGOH010000820.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4210)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4210)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4210
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4705107"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="AC159P_15855"
     CDS             complement(<1..224)
                     /locus_tag="AC159P_15855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696971.1"
                     /translation="MLIVTLKLTVTRLRQQALPLWLCLALSGGWCLMSRSVAGQTQIA
                     GAQTASTETKEAERLNRQVVELYREGRYAEA"
     gene            complement(816..1262)
                     /locus_tag="AC159P_15860"
     CDS             complement(816..1262)
                     /locus_tag="AC159P_15860"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3696972.1"
                     /translation="MILLADLNAEREEAVRREMKPKVLIIDDDVAVTQQLFWTLCEEF
                     EVMTANNLASAIRRATIYEPEIAILDLHLPPTLDAPDTGLRILEYVKAHLPTSKVFVV
                     SSSATVEQQKDCIRRGADGFLSKPLDVEHLLSTVRRTALARRLEAA"
     gene            1639..2232
                     /locus_tag="AC159P_15865"
     CDS             1639..2232
                     /locus_tag="AC159P_15865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696973.1"
                     /translation="MGTSGVKGQTDKFARLRKNRRVNMRFPVTVEVPVGGGRSRRLHA
                     HTLVVSHAGATLDLDEGIPVEAGIQVTPPFGGAILAEVTDAWVDRSSGRHRVSIRLID
                     PTSWTSPDRLASAPVVAGRDAHSLFLSPRAWQMLDDYAAYLAERDGAGLSATDTAERI
                     VEQVFLSDANFQNWFAAKIMEDLQAWEEASVVAVEKQ"
     gene            complement(2408..3832)
                     /locus_tag="AC159P_15870"
     CDS             complement(2408..3832)
                     /locus_tag="AC159P_15870"
                     /inference="COORDINATES: protein motif:HMM:NF025885.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HIR3696974.1"
                     /translation="MSEQTEQKTTVLIVDDDQLLRRQLHWALVERHRVLEAETRPQAI
                     DLLRRERVDVVLCDLHLPPDTGGIEEGLAVIDAARDARPSVPVVVITGSDKKQAALEA
                     VRRGAYGFFEKPFDEEEVSHIVVQAARLRKLEAEVLRLRSELEMFRGFGRFVGTSAAL
                     DAVLKQARAVADTNATLLITGENGTGKEVLARAVHEESARRDRAFVAVSCAALPEQLI
                     ESELFGHEKGAFTGATQARKGRFELADGGTLFLDEIGELTPAVQVKLLRVLQERSFER
                     VGGTKTLEVDIRLIAASNRDLEAEVEAGRFRRDLFYRLNVVPLCLPPLRERRDDIPTL
                     AAHFATKGAEKHNRPVPALDPALLEALQEYDWPGNIRELENLVERLVVLTRDQALGVE
                     HLPERMLLRAAPTEGATAGTASATPAAADETTLEGAMLALKRRMLVGALQAEGGNRAA
                     AAKRLHISRSYLHRLIGELGINGL"
     gene            complement(4032..4205)
                     /locus_tag="AC159P_15875"
     CDS             complement(4032..4205)
                     /locus_tag="AC159P_15875"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR3696975.1"
                     /translation="MGLGLFTCREIVEAHGGRLEVESKPGAGTCFRVVLPSRLFASGE
                     RFKSRSSEGAVGS"
BASE COUNT          688 a         1349 c         1459 g          714 t
ORIGIN      
        1 gcctccgcgt atctgccttc gcggtacaat tcgacaacct gccggttaag cctctcggct
       61 tccttcgtct ccgtggacgc agtctgcgcg ccggcgattt gagtctgccc ggccacgctg
      121 cgcgacataa ggcaccagcc cccggacagc gccaaacaca gccagagggg caaggcctgc
      181 tgacgcaacc gcgttacggt aagttttagg gtgacgatta acattgggcc accttactaa
      241 accgaagccc ggctactcgc tccggcgccg aagtagcgcc ggaagggccg aaggttgaag
      301 taagacggga ggcgcgaggg cacgcaggct cgaccgccag cctgacacgc gagcgagggc
      361 gcaaatctat cgtcgtcggg agcggcctgt caatctaatt cgagggaggg gcgtgccgga
      421 ggcggtcgtc aaccgaatcc agggccactc gaaaccccgc atgacggttc atcacctcca
      481 cgtgccggaa ggctcgacgc gcgaggccgt gggaagagcg cccactttcc gtcacaacga
      541 agtgccgaaa attgccagct tcgggcacaa aaataaagcc cccgcgactc tgggcaggag
      601 tctttttcgg agacgggggc ttttgcgctc cgcgcatcgg ttgaagggat accgttggcg
      661 cgcggcgcgg ctacggcctt cgaagaaggg gtgcgaaggc cgaagtgaat tctcaaagag
      721 cgcgcggcgc gggcggtgag ggcctcttga gtatgctttc gcgccgcgtt gtcaaagggc
      781 aacttgcaac ccgtgagggg gctttcgtga gttcgtcagg cggcttcgag ccgccgcgcg
      841 agcgccgtcc ggcgcacggt cgagagcagg tgttccacgt cgagcggttt gctcaggaag
      901 ccgtccgcgc cgcggcggat gcagtccttc tgctgctcga cggtggctga cgacgaaacg
      961 acgaagacct tcgacgtggg caggtgcgcc ttgacgtatt cgaggatgcg caggcccgtg
     1021 tccggcgcgt cgagcgtggg cggcaggtgc aggtcgagga tcgcgatctc cggctcgtag
     1081 atggtcgcgc gccggatggc cgaggcgagg ttgttggccg tcatcacctc gaactcctcg
     1141 cacagcgtcc agaacaactg ctgcgtcacc gccacgtcgt cgtcaataat caaaaccttg
     1201 ggcttcatct cgcgccgcac cgcctcttcc cgctccgcat tcaagtccgc cagtaaaatc
     1261 atgatctgtt cgaatcaccc caatctacgt cgtcttcacg accggcggat tttcaactgc
     1321 gtcgcccgtt ccggttttca actttcgcaa cacccgtgcc actcgcccgc gccgacgttt
     1381 tacgggcaaa ttcgaggaca cgcgctgtac gccccgcgcg aagtgtgaac gggagtgaac
     1441 agcgcggccc ggcgcgtgtg cacaactgtt gacagggacg cggcggacgg cggcgggggg
     1501 gcgcggttcg cggaagggtc tgacgacgag aggcatcgtg gcccgagtcc gcggggcgcg
     1561 gcgggcggca ccgcagttgc agatggtgtg aggcggggcc gttcggagtc tgaacatgga
     1621 gcaaaacagg tctttatgat gggaaccagc ggagtcaaag gtcaaactga taagttcgcg
     1681 cggctgcgga agaacaggcg cgtcaacatg cgcttcccgg tcacggtcga agtgccggtg
     1741 ggcgggggcc gctcgcgccg cctgcacgcg cacacgttgg tcgtgagcca cgctggcgcg
     1801 acgctcgacc tggacgaggg catcccggtc gaggcgggca tacaggtgac gccgccgttc
     1861 ggcggggcca tactcgccga ggtcacggac gcgtgggttg accgctccag cggtcggcac
     1921 cgcgtcagca tccgcctcat cgaccccacg tcgtggactt cgcccgacag gctcgcgtcg
     1981 gcgcccgtcg tcgccggtcg cgacgcgcac agcctctttc tcagcccgcg cgcgtggcag
     2041 atgctcgacg actacgcggc ctacttggcc gagcgcgacg gcgccgggct gtccgccacc
     2101 gacacggcgg agcgcatcgt cgagcaggtc ttcctgtccg acgcgaattt ccagaactgg
     2161 ttcgccgcga agatcatgga agacttgcag gcgtgggagg aggcgagcgt cgtcgcggtg
     2221 gaaaagcagt aggcggtagg cagtaggcgg taggcagtaa aagcagaagt cagaagagcg
     2281 aagaggcttg gtaggtaaag tcccaagcct cttcgcccga caagcagtga agtgaggagg
     2341 cttgagacgt ttaatctcaa gcctctttcc tctttttctt ttaactgcct accgcctact
     2401 tcttccttca gaggccgttg atgccgagtt cgccgatgag gcggtggagg taggagcggc
     2461 tgatgtggag ccgcttggcg gcggcggcgc ggttgccgcc ctcggcttgg agcgcgccga
     2521 cgagcatccg gcgcttgagc gcgagcatcg cgccttcgag cgtcgtctcg tcggcggcgg
     2581 cgggcgtcgc ggaagccgtg ccggcggtcg cgccctctgt cggcgcggcg cgcagcagca
     2641 tccgctcggg caggtgctcg acgccgagcg cctggtcgcg cgtcaggacg acgagccgtt
     2701 cgaccaggtt ctcaagctcg cggatgttgc ccggccagtc gtactcctgc aaggcttcga
     2761 ggagcgcggg gtcgagcgcg gggacggggc ggttgtgctt ttcggcgccc ttcgtggcga
     2821 agtgcgcggc gagggtcggg atgtcgtcgc gccgttcgcg cagcgggggc aggcacaggg
     2881 ggacgacgtt caggcggtag aagaggtcgc ggcggaagcg gcccgcctcg acttccgctt
     2941 cgaggtcgcg gttcgaggcg gcgatcagac ggatatcgac ctcgagcgtc ttcgtcccgc
     3001 cgacgcgctc gaacgagcgc tcttgcagca cgcgcaggag cttgacctgc acggcgggcg
     3061 tcagctcgcc gatctcgtcg aggaagagcg tgccgccgtc ggccagctcg aagcgcccct
     3121 tgcgggcctg cgtggcgccg gtgaaggcgc ccttctcgtg gccgaacagc tcgctctcga
     3181 tgagctgttc gggcagcgcc gcgcaggaga cggcgacgaa ggcgcggtcg cgccgcgcgc
     3241 tctcttcgtg gacggcgcgc gccaagacct ctttgcccgt cccgttctcg cccgtgatga
     3301 ggagcgtcgc gttcgtgtcg gcgacggcgc gcgcctgctt cagcaccgcg tcgagcgccg
     3361 cgctcgtccc gacgaagcga ccgaagccgc ggaacatctc cagctccgag cgcagccgca
     3421 ggacttcggc ttcgagcttg cgcaggcgcg ccgcctgcac gacgatgtgc gagacctcct
     3481 cttcgtcgaa gggcttctcg aaaaacccgt aggcgccgcg gcggacggct tcgagcgcgg
     3541 cctgcttctt gtcgctcccc gtgatgacga cgacggggac gctgggccgt gcgtcgcgcg
     3601 cggcgtcgat gacggcgaga ccttcctcga tgccgccggt gtcgggcggc aggtgcaggt
     3661 cgcagagcac cacatccacg cgctcgcgcc gcagcaggtc gatggcctgc gggcgcgtct
     3721 cggcttcgag cacgcggtgc ctctcgacga gtgcccagtg gagctgccgc cgcagcagtt
     3781 gatcgtcgtc cacgatgagc acggtcgtct tctgttcggt ctgttcgctc acggttttgc
     3841 ggcgctctcc gggcgcgcat cttcggggcc gagtcaccgc ccgcttcgcg gcagtcttcc
     3901 gaccgccggt gcaagcgacg aggtaagttg aaacgacggc ccgcagcccg tccctctcga
     3961 acggtcggcc ttgttcgtct agtgtctgga aaagctgaaa ggagttatgc gcccgccgtc
     4021 gcgatccgtc ttcaactgcc gaccgcccct tctgacgatc tcgacttgaa ccgctcgccc
     4081 gacgcaaaca ggcgggatgg taacacgacg cggaagcacg tgccagcgcc gggtttcgac
     4141 tcgacttcga gccgaccgcc gtgcgcctcg acgatctcgc ggcaggtgaa gaggccgagg
     4201 ccgatgccct
//
LOCUS       DBNGOH010000821         1831 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3239761, whole genome shotgun sequence.
ACCESSION   DBNGOH010000821 DBNGOH010000000
VERSION     DBNGOH010000821.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1831)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1831)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1831
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3239761"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..912)
                     /locus_tag="AC159P_15880"
     CDS             complement(<1..912)
                     /locus_tag="AC159P_15880"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HIR3696976.1"
                     /translation="MRIPFLDLRRQLEEIGAEVDDALRRVLRGGAYILGPEVAAFEEE
                     WARFCGARFAAGVGNGTDALALALLASGAVRPGAGDEVVTTPLTAAYTALAILNAGAV
                     PVFADVDPQTLTLSPAALDAAVTPRTRAVIPVHLYGQTADIGGIYEVAARRDLFILED
                     AAQAHGASYRGKLTGGLGFAAAFSFYPTKNLGAYGDGGAVTSNNEELIERVKTLREGG
                     HAAALGGEVAGMNSRLDEAQAAVLRVKLARLEDWNQRRRRIAALYQNAFAGTSITTPL
                     ERGPFSHVFHLYVVRHPDRDRLRAHLTA"
     gene            complement(875..>1831)
                     /locus_tag="AC159P_15885"
     CDS             complement(875..>1831)
                     /locus_tag="AC159P_15885"
                     /inference="COORDINATES: protein motif:HMM:NF027681.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR3696977.1"
                     /translation="KLVEVGDVEVTVLDALLPDQGGNPYNVRDVKDRLQVRVEDMGDD
                     WVINHLVGGVDYIFNLAGNVSHLDSMQYPQRDLELNCTAQLALLEACRNFNPHVKIVF
                     TSTRQVYGKPLYLPLDEEHRVSPVDINGINKLAAENYHLLYHRVYGTRTVCLRLTNTY
                     GPRQLLRHNRQGFIGWFVRQAVEGGAIELYGEGKQRRDLNYVDDVVEALLLAGASEAA
                     EGEVFNLGGAEPTALCDLAHELIALTGRGTARCVPFPVERQLIDIGNSYSSFGKIEAA
                     LGWRPRTPLREGLARTVEFYRQHGAHYWNKDADTLSRPSATA"
BASE COUNT          257 a          564 c          703 g          307 t
ORIGIN      
        1 ggcggtgagg tgtgcgcgca ggcggtcgcg gtcggggtgg cggacgacgt agaggtggaa
       61 gacgtgcgag aaagggccac gctctagggg cgtggtgatg gaagtaccgg cgaaggcgtt
      121 ctggtagagc gcggcgatgc ggcggcggcg ttggttccag tcttcgaggc gcgcgagttt
      181 gacgcgcaag acggcggcct gcgcttcgtc gagacgcgag ttcatgccgg ccacttcccc
      241 gccgagtgcc gccgcgtgtc cgccctctcg cagggtcttg acgcgttcga tgagttcttc
      301 gttgttcgac gtgacggcgc cgccgtcgcc gtaggcgccg aggtttttcg tcgggtagaa
      361 gctgaaggcc gcggcgaagc cgagtccgcc cgtgagtttg ccgcggtagg acgcgccgtg
      421 cgcctgcgcc gcatcttcga ggatgaagag gtcgcggcgc gcggcgactt cgtagatgcc
      481 gccgatgtcg gcggtctgcc cgtagaggtg gacggggatg acggcgcgcg tgcgcggcgt
      541 gacggcggcg tcgagcgcgg cgggcgagag cgtgagcgtt tgcgggtcta cgtcggcgaa
      601 gacggggacg gcccctgcgt tgaggatggc gagcgcggtg taggcggcgg tcagaggcgt
      661 ggtgacgact tcgtcgcccg cgccggggcg caccgcgccg gaggcgagca gcgcgagcgc
      721 gagcgcgtcg gtgccgttgc cgacgcccgc cgcgaagcgc gcgccgcaga agcgcgccca
      781 ctcctcttcg aacgctgcga cctctgggcc gaggatgtag gcgccgccgc ggagcacgcg
      841 ccggagcgcg tcgtccacct ccgcgccgat ctcttcaagc tgtcgccgaa ggtcgagaaa
      901 gggtatccgc atccttgttc cagtagtggg cgccgtgctg ccggtagaac tcgacggtgc
      961 gcgccagccc ttcgcgcaga ggggtgcgcg ggcgccaccc gagcgccgcc tcgatcttgc
     1021 cgaagctcga atagctgttg ccgatgtcga tgagctgccg ctcgacgggg aaagggacgc
     1081 agcgcgccgt gccgcgtccc gtgagcgcga tcagctcgtg cgcgaggtcg cagagcgcgg
     1141 tcggctcggc gccgccgagg ttgaagactt cgccctccgc ggcctcgctc gcgcccgcga
     1201 gcaggagcgc ctccacgaca tcgtccacgt agttcaggtc gcgccgctgc ttgccctcgc
     1261 catagagttc gatggcgccg ccctcgaccg cctgccgcac gaaccagccg atgaagccct
     1321 gccggttgtg gcgcagcagt tggcgcgggc cgtaggtgtt cgtcaaacgc aggcagacgg
     1381 tgcgcgtgcc gtagacgcgg tggtagagca ggtggtagtt ctcggcggcg agcttgttga
     1441 tgccgttgat gtccacgggc gagacgcggt gctcctcgtc gagcggcagg taaagcggct
     1501 tgccgtagac ctgccgcgtg ctggtgaaga cgatcttcac gtgcgggttg aagttgcggc
     1561 aggcttcgag gagcgcgagc tgggccgtgc agttgagttc gaggtcacgc tgcgggtact
     1621 gcatcgagtc gaggtggctg acgttgcccg caaggttgaa gatgtagtcc acgccgccga
     1681 cgaggtggtt gatgacccag tcgtcgccca tgtcctcgac gcgcacctgc aagcggtctt
     1741 tcacgtcgcg gacgttgtag gggttgccgc cctggtcggg cagcagcgcg tcgaggacgg
     1801 tcacttccac gtcgcctacc tcgacgagct t
//
LOCUS       DBNGOH010000822         2466 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1041619, whole genome shotgun sequence.
ACCESSION   DBNGOH010000822 DBNGOH010000000
VERSION     DBNGOH010000822.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2466)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2466)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2466
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1041619"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..321
                     /locus_tag="AC159P_15890"
     CDS             <1..321
                     /locus_tag="AC159P_15890"
                     /inference="COORDINATES: protein motif:HMM:NF012241.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HIR3696978.1"
                     /translation="WIPAADVYEGEGEYTLALDLPGIRREALDVSLDEGRLTIRGERA
                     PAPEGVVARRTERAAGRFVRSFILPEAVDRQSIAADYKDGVLHLRLPKRTEQAPRRVK
                     IDIN"
     gene            487..1158
                     /locus_tag="AC159P_15895"
     CDS             487..1158
                     /locus_tag="AC159P_15895"
                     /inference="COORDINATES: protein motif:HMM:NF013212.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HIR3696979.1"
                     /translation="MEETRGAADELHAEEGADPAGERVRVTDRRRVSLNDIEGGGEGR
                     AADEGPSLKPSYVEELEARTRAAESAVADVQARFDQLRAELRRETDEVRQRLTRAGEE
                     RAARERANFLASLLPVMDNLRRAVEAAEDGGSLESLLDGLRGTIGGFESLLTNAGAEP
                     IEAVGERFDPELHEAVDTTEVEPEREGEVTAQYSRGYRMGKQLLRPARVQVGRARGGA
                     QAAGE"
     gene            1247..>2466
                     /gene="groL"
                     /locus_tag="AC159P_15900"
     CDS             1247..>2466
                     /gene="groL"
                     /locus_tag="AC159P_15900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011554876.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HIR3696980.1"
                     /translation="MLEGVNVLANAVRVTLGPRGRNVVLGKKYGSPVITKDGVTVAKE
                     IELKDKYQNMGAQMVREVASKTNDMAGDGTTTATVLAQALYREGVKNVAAGANPMSLQ
                     RGIQRGVEAATAALEKMSKKVKDKSDLANVAAVSANNDREIGELVSEAMERVGRDGVV
                     TVEESRTLQTELDLVEGMQFDRGYLSPYFVTNPDRMESALEEPLILLHEKKIAAMRDL
                     LPVLEQAAQQGRALLIIAEDVEGDALATLVVNRLRGTIKVCAVKAPAFGDRRKAILED
                     LAVLTGGRVITEDLGIKLDSVTLEDLGSAKRVIVDKDNTTIVEGAGNSKEIQGRVATL
                     RRQVEETTSDYDREKLQERLAKLAGGVAVIRVGAPSESEMKARKDALDDAISSTKAAV
                     AEGIVPGGGLALIR"
BASE COUNT          449 a          743 c          944 g          330 t
ORIGIN      
        1 tggattccgg cggcggacgt gtacgagggc gagggcgagt acacgctcgc gctcgacctg
       61 ccgggcatcc ggcgcgaggc gctggacgtg agcctcgacg aggggcgtct gaccatccgc
      121 ggcgagcgcg cgcccgcgcc cgaaggcgtg gtcgcgcggc gcacggagcg cgcggcgggt
      181 cgcttcgtcc gcagcttcat tctgccggag gccgttgacc gccagtcgat cgcggcggac
      241 tacaaggacg gcgtgcttca tctgcgactg cccaagcgca cggagcaggc gccgcgccgc
      301 gtcaagatcg acatcaacta aagcggtgaa tggtgactgg tgaatagtgc atggtgttcg
      361 agagtcgttg cggtgtgagc ggtaggcggc aagtcgtgcg gcgagcgcgc atttcgattt
      421 accatttacc atttaccaat tactattcag gacatgtttt tccgtggggg caaaaagagg
      481 cgacgtatgg aagagacgag gggggctgcg gacgagcttc acgccgagga gggtgccgac
      541 cccgccggcg agcgcgtgcg cgtcacggac cggcggcggg tcagcctgaa cgacatcgag
      601 ggcggcggcg agggccgcgc cgcggacgag gggccgagcc tgaagccgag ctacgtcgaa
      661 gagcttgagg cgcgcacccg cgcggcggag tcggcggtcg cggacgtgca ggcgcgcttc
      721 gaccagttgc gcgcggagct gcggcgcgag accgacgagg tgcggcagcg tctgacgcgc
      781 gcgggcgaag agcgcgcggc gcgcgagcgg gcgaacttcc tcgcgtcgct gctgcccgtg
      841 atggacaacc tgcgacgcgc ggtcgaggcg gcggaggacg gcggctctct ggagtctctg
      901 ctcgacggcc tgcgcggcac catcggcggc ttcgagagcc tgctcacgaa cgcgggcgcc
      961 gaacccatcg aggccgtcgg cgaacggttc gacccggagc tgcacgaagc ggttgacacg
     1021 accgaggtcg agccggagcg cgagggagag gtcacggcgc agtacagccg cggctaccgc
     1081 atgggcaaac aactcttgcg gcccgcgcgc gtgcaggtcg gtcgcgcgcg cggcggggcg
     1141 caggcggcgg gcgagtagtt cggctgttag aggcggatta attcgtaagg agaaaacata
     1201 agtggcagcg aaggatttga agttcaggga agaggcgcgg cgcgcgatgc tggaaggcgt
     1261 gaacgtgctg gcgaacgcgg tgcgcgtgac gctggggccg agggggcgga acgtggtgct
     1321 cggcaagaag tacggctcgc cggtcatcac gaaggacggc gtgacggtgg cgaaagagat
     1381 cgagctgaag gacaagtatc agaacatggg cgcgcagatg gtgcgcgagg tcgcttcgaa
     1441 gacgaacgac atggcgggcg acgggacgac caccgcgacc gtgctcgcgc aggcgctcta
     1501 ccgcgagggc gtgaagaacg tcgcggcggg cgccaacccg atgtccttgc agcgcggcat
     1561 ccagcggggc gtcgaggcgg cgaccgccgc gctcgaaaag atgtcgaaga aggtgaagga
     1621 caagtccgac ctcgccaacg tcgccgccgt gtcggcgaac aacgaccgcg agatcggcga
     1681 gctggtctcc gaggcgatgg agcgggtcgg cagggacggc gtcgtgacgg tcgaagagtc
     1741 gagaacgctc cagaccgagc ttgacttggt cgagggcatg cagttcgacc gcgggtatct
     1801 gtcgccctac ttcgtcacca accccgaccg catggagtcc gcgctggaag agccgctgat
     1861 tcttctgcac gagaagaaga tcgcggcgat gcgcgacctg ctgccggtgc tcgaacaggc
     1921 ggcgcagcag gggcgcgcgc tgctcatcat cgcggaggac gtggagggcg acgcgctcgc
     1981 cacgctcgtc gtcaaccgcc tgcgcggcac gatcaaggtc tgcgccgtca aggcgcccgc
     2041 cttcggcgac cgccgcaagg ccatcctcga agacctcgcg gtgctgaccg gcgggcgcgt
     2101 catcaccgaa gacctcggca tcaagctcga cagcgtgacg ctcgaagacc tcggctccgc
     2161 gaagcgtgtc atcgtcgata aggacaacac gaccatcgtc gagggcgcgg gcaacagcaa
     2221 agagattcag gggcgcgtgg cgaccctgcg ccggcaggtg gaggagacga cctcggacta
     2281 cgaccgcgag aagttgcagg agcggctggc gaagctcgcc gggggcgtcg ccgtcatccg
     2341 cgtcggcgcg ccgtccgagt ccgagatgaa ggccaggaag gatgcgctgg acgacgccat
     2401 cagctcgacc aaggctgccg tcgccgaagg catcgtgccc ggcggcgggt tggcgctgat
     2461 ccgctg
//
LOCUS       DBNGOH010000823         2165 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4338529, whole genome shotgun sequence.
ACCESSION   DBNGOH010000823 DBNGOH010000000
VERSION     DBNGOH010000823.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2165)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2165)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2165
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4338529"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(85..306)
                     /locus_tag="AC159P_15905"
     CDS             complement(85..306)
                     /locus_tag="AC159P_15905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3696981.1"
                     /translation="MLLFRSGEHVGRWCEAWRLQSGESFSVELCWRLARAWFDRNRGE
                     PDWRRRTPDEAEGVFASLGLTSPFWRLKS"
     gene            complement(313..774)
                     /gene="merB"
                     /locus_tag="AC159P_15910"
     CDS             complement(313..774)
                     /gene="merB"
                     /locus_tag="AC159P_15910"
                     /EC_number="4.99.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF015216.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018836 - alkylmercury lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="organomercurial lyase"
                     /protein_id="HIR3696982.1"
                     /translation="MSEDQNQENFDREVRRHVYDRVINEGLPPSAAGTAAALGRPRED
                     VRASFRRLAEAHVLVLQEGGGEVLMAAPFSAVPTPFVVRSRARKWYANCVWDALGIPA
                     MLKCDARVEASCGCCGTAMRLSVRGGALEEAAGVAHFALPAARWWDDVVFT"
     gene            1035..>2165
                     /locus_tag="AC159P_15915"
     CDS             1035..>2165
                     /locus_tag="AC159P_15915"
                     /inference="COORDINATES: protein motif:HMM:NF024753.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HIR3696983.1"
                     /translation="MREGERSFGLKPLVMCAVVACVVVLALAGQTFRAARGEGGTKAK
                     KTAAGRELKIDLNRSVEVKLPEFKGDLKPVAFRTKDGREGWAVKIPGGLPIATPAYAD
                     GRLFVGGGYGSHEFYAFDAKTGRLVWQMKTSDDGPSAAVVEDNYVAFNTESCTLIVTD
                     ARTGKLVWEEWLGDPLMSQPAISKGRVFMAYPAGQRGGHGRGRILTNKKKAKQSAQDV
                     GQGSHRLLAADLRTGEHIWERAISADVISAPVISEDKVYFTTFVGDSYCLDARNGRVV
                     WKKENAGTSAPVVVAGEVVVTQKEARGNKSYEGLKRLDAARGADKDKALIASDKAGYL
                     DENKGGGVALQQEAVQALDSSVGFAQAPAAAELGAANRHLGVR"
BASE COUNT          372 a          686 c          776 g          331 t
ORIGIN      
        1 tgtcgcgccg gtgcagctct tcgatcgccg ccaaatcttc ttcgtggctc atccgttcgt
       61 cgtcttcgtc cgcggcgcgc ggcctcaact cttcaggcgc cagaacggcg aggtcaggcc
      121 gagcgaggcg aagacgcctt cggcctcgtc cggcgtgcgc ctgcgccagt ccggctcgcc
      181 gcggttacgg tcgaaccagg cgcgcgccag tcgccagcac agctcgacgg aaaaactctc
      241 gccgctctga aggcgccacg cctcgcacca gcgcccgacg tgctctcccg accggaagag
      301 gagcatcgtc tttcaagtga agaccacgtc gtcccaccag cgcgccgcgg gcagcgcgaa
      361 gtgcgcgacg cccgcggcct cttcgagcgc gccgccgcgc accgagaggc gcatcgccgt
      421 cccgcagcac ccgcacgacg cctcgacgcg ggcgtcgcac ttcagcatcg ccgggatgcc
      481 gagcgcgtcc cacacacagt tcgcgtacca cttgcgcgcg cgcgacctga cgacgaacgg
      541 cgtcggcacc gccgagaagg gcgccgccat cagcacctcg ccgccaccct cctgcaagac
      601 gagcacgtgc gcctcggcga ggcggcggaa ggaggcgcgc acgtcttcgc gcggacgccc
      661 gagcgccgcc gccgtgcccg cggcgctcgg cggcagcccc tcgttaatca cgcggtcgta
      721 gacgtggcgg cggacttcgc ggtcgaaatt ctcttggttt tgatcttcac tcatgcggca
      781 gatgcttcta tgccaaacgt cgcggaaagt caacgggaaa gcggttttcg gttttcggtt
      841 ccagttttca gcgaggacag cattcagctt ttagctgtta gctgttagct ttcggcaaga
      901 agcgtcgtct tcactgaaaa ctgaaaactg cttcccgcaa gttatcggcg ccgggcgcat
      961 tctacctgta actagcgtga gtctcccgcg cagggtcgag gggagtcgaa atcgttcgaa
     1021 ggcggaggtg tgtgatgaga gagggcgaac gttcgttcgg gttgaagccg ctcgttatgt
     1081 gcgcggtcgt cgcgtgcgtg gtcgtgttgg cgttggctgg tcagacgttt cgcgcggcgc
     1141 gcggagaggg cgggacgaag gcgaagaaga cggcggcggg cagggagttg aagatcgacc
     1201 tgaacaggtc ggtcgaggtg aagctgccgg agttcaaggg ggatttgaag ccggtcgcgt
     1261 tcaggacgaa ggacggcagg gaagggtggg ccgtcaagat tccgggcggg ctgccgattg
     1321 cgacgccggc ctacgcggac gggcgtctgt tcgtcggcgg cggctacggc tcgcacgagt
     1381 tctacgcctt cgacgcgaag acgggtcggc tcgtctggca gatgaagacc tcggacgacg
     1441 ggccgagcgc ggcggtcgtc gaggacaact acgtcgcctt caacacggag agctgcacgc
     1501 tcatcgtgac cgacgcgcgc accggcaagc tcgtgtggga ggagtggctc ggcgacccct
     1561 tgatgagcca gcccgccatc tcgaaggggc gcgtcttcat ggcctacccg gcgggccagc
     1621 gcggcggcca cgggcgcggg cgcatcctca ccaacaaaaa gaaggcgaag cagagcgcgc
     1681 aggacgtggg gcagggcagc caccgcctgc tcgcggcaga cctgcggacg ggcgagcaca
     1741 tctgggagcg cgccatctcg gcggatgtga tctcggcgcc ggtcatctcc gaagacaagg
     1801 tctacttcac gaccttcgtc ggcgactcgt actgcctcga cgcgcgcaac gggcgcgtcg
     1861 tgtggaagaa ggagaacgcg gggacgagcg cgccggtcgt ggtcgcgggc gaggtggtcg
     1921 tgacgcagaa ggaggcgcgc ggcaacaagt cttacgaagg gctgaagcgg ctggacgcgg
     1981 cgcgcggcgc cgacaaggac aaggcgctga tcgcgagcga caaggccggc tatctcgacg
     2041 agaacaaggg cggcggcgtc gccttgcagc aggaggcggt gcaggcgctc gactcgtcgg
     2101 tcggtttcgc gcaggcgccc gcggcggcgg agctgggcgc ggcgaacaga cacctcggcg
     2161 tcagg
//
LOCUS       DBNGOH010000824         3503 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2322545, whole genome shotgun sequence.
ACCESSION   DBNGOH010000824 DBNGOH010000000
VERSION     DBNGOH010000824.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3503)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3503)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3503
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2322545"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1617)
                     /locus_tag="AC159P_15920"
     CDS             complement(<1..1617)
                     /locus_tag="AC159P_15920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015460034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3696984.1"
                     /translation="MDNSLGAVVGGETRAGAGVSAWSPLRHPAFRALWVAMLAANVGT
                     WMQQVGAAWLMTSLGASATMIALVQTATSLPVFFLALPAGTFADIFDRRKLLLWSQAW
                     MLLAAALLAALTYAGATTPWVLLVFTFAVGAGTAVGLPAFQAVVPEILPKEEMAAAFT
                     VSGVAMNIARAVGPALSGMVIAAAGAEAVFALNALSFLAALYVIRRWRREQIRTELPP
                     ERVLRAMRAGLRYARHAPALQSVLVRTGAFIFGASALWALLPLRAQRELGTSAAGYGV
                     LLGAIGVGALGGAFVLAWLRRRLRLDALMAAASLVYAAATVALVLLQDFYVLCAAMMA
                     CGAAWMAIMSSLNTAAALSVPAWVKARALALYLLVFQGGLALGSFVWGFVAERAGTGA
                     ALVAAGATLAAGVLAGLRYRLSNVGDAELEPALHWDAPQVASAFDGLAQEGEGRASMV
                     TVEYYIDPRRAGEFRAAMRGVRAERMRHGATYWGLFADASDPSRWVEFFAQPSWTEHL
                     RQHERVTQADRDMEARALAFHILAEPPRATH"
     gene            complement(1635..2414)
                     /locus_tag="AC159P_15925"
     CDS             complement(1635..2414)
                     /locus_tag="AC159P_15925"
                     /EC_number="1.13.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016701 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DODA-type extradiol aromatic ring-opening family
                     dioxygenase"
                     /protein_id="HIR3696985.1"
                     /translation="MGVEVFPSVFVSHGAPTLIIERNETVEFLKGLGEELGRPRAILC
                     VSAHWETRVPTLSAAERPETIHDFGGFPEELYRMRYDAPGAPELAARACELLKAAGHS
                     CELSPGRGLDHGAWVPLSLMYPAADIPVTQLSVQPHAGPAAHVRVGRALEPLRREGVL
                     VLASGGAVHNLSTFRRATTTPDWAARFDEWLHEKLTAGAFEDVIDYRALSAEARAAHP
                     TEEHLLPLFVALGAGGAAARVRPLHRGFTHGSFSMAAYAFG"
     gene            complement(2505..2945)
                     /locus_tag="AC159P_15930"
     CDS             complement(2505..2945)
                     /locus_tag="AC159P_15930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIR3696986.1"
                     /translation="MTKPFASREEEVYLNIQRTAEALWWGVNETLKRADLTPTQYNVL
                     RILRGAGEAGASCGEISERLVTKDSDITRLLDRLEARGLISRGREAKDRRRITARITE
                     EGLRILAELDRPVAQCHRRQLSHLGEKRLAALSKLLDAARDRPE"
     gene            complement(3217..>3503)
                     /locus_tag="AC159P_15935"
     CDS             complement(3217..>3503)
                     /locus_tag="AC159P_15935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004571 - mannosyl-oligosaccharide
                     1,2-alpha-mannosidase activity [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 47 protein"
                     /protein_id="HIR3696987.1"
                     /translation="PEIVESTYYLFRYTRDPRYLRMGETLFRDFVKHCRTDEAYAALK
                     SVVTKEKSDSMQSFLFAETFKYFYLLFAPPRTLDFDAVVFNTEAHPIRKE"
BASE COUNT          580 a         1325 c         1117 g          481 t
ORIGIN      
        1 gtgcgtcgcg cgcggcggct ctgcgaggat gtggaaggcg agggcgcgcg cctccatgtc
       61 gcggtcggcc tgcgtcacgc gctcgtgctg gcgcaggtgc tccgtccacg aaggctgcgc
      121 gaagaactcg acccagcgcg aagggtccga ggcgtccgcg aacagccccc agtaggtcgc
      181 gccgtggcgc atccgctcgg cgcgcacgcc gcgcatcgcc gcgcggaact cgcccgcgcg
      241 ccgcgggtcg atgtaatact ccaccgtcac catcgaggcg cggccctcgc cctcttgcgc
      301 gaggccgtcg aacgccgaag cgacctgcgg cgcgtcccag tgcagcgccg gctcaagctc
      361 cgcgtcgccg acgttcgaca ggcggtaacg cagacccgcg agcacgcccg cggccagcgt
      421 cgcgcccgcc gcgacgagcg ccgcgcccgt gcccgcgcgc tccgcgacga agccccacac
      481 gaaactcccg agcgccagcc cgccctgaaa caccagcagg tagagcgcca gcgcgcgcgc
      541 cttgacccac gccggcacgg acagcgccgc cgccgtgttc aggctcgaca tgatcgccat
      601 ccacgccgcg ccgcacgcca tcatcgccgc gcacagcacg tagaagtctt gcaagaggac
      661 gagcgccacc gtcgccgccg cgtagacgag cgacgccgcg gccatcagcg cgtccagcct
      721 cagccgccgc cgcagccacg cgagcacgaa cgcgccgccg agcgccccga ccccgatggc
      781 gccgagcagc acgccgtaac cggcggcgct cgtccccagc tcgcgctgcg cgcgcagcgg
      841 cagcagcgcc cacagcgcgc tcgctccgaa gatgaaggcg cccgtcctca cgagcaccga
      901 ctgaagcgcg ggcgcgtggc gcgcgtagcg caatcccgcg cgcatcgcgc gcagcacgcg
      961 ctccggcggc agctccgtcc gaatctgctc gcgccgccag cgccggatga cgtaaagggc
     1021 ggcgaggaac gacagcgcgt tcagcgcgaa gaccgcctcg gcacccgccg ccgcgatgac
     1081 catgccgctc aacgccgggc cgaccgcgcg cgcgatgttc atcgccacgc ccgacaccgt
     1141 gaaggccgcc gccatctcct ccttcggcag aatctcgggg acgaccgcct ggaacgcggg
     1201 cagcccgacc gccgtccccg ccccgaccgc gaaggtgaag accagcaaga cccacggcgt
     1261 cgtcgcgccc gcgtacgtga gcgccgcgag cagcgccgcc gccaacagca tccacgcctg
     1321 cgaccagagc agcagcttgc ggcggtcgaa gatgtccgcg aacgtcccgg cgggcagcgc
     1381 gaggaagaag accggcagcg aggtggccgt ctgcacgagc gcgatcatcg tcgccgacgc
     1441 gccaagcgaa gtcatcagcc acgccgcgcc gacctgctgc atccacgtcc ccacgttcgc
     1501 cgcgagcatc gccacccaca gcgcgcggaa agcgggatgg cgcagcggac tccacgcgga
     1561 cacccccgcg cccgcacgcg tctcgccccc gaccacagca cccaacgagt tatccaaagc
     1621 agtccccaac tcgctcaccc gaacgcatag gccgccatgc tgaagctgcc gtgcgtgaag
     1681 cccctgtgca gcgggcgcac gcgcgccgcc gcgccgcccg ccccgagcgc gacgaagagc
     1741 ggcagcaaat gctcttcggt cgggtgcgcc gcccgcgctt ccgcgctcag cgcgcggtag
     1801 tcgatcacgt cctcgaacgc gcccgccgtg agcttctcgt ggagccactc gtcgaaccgc
     1861 gcggcccagt cgggcgtcgt cgtcgcgcgg cggaacgtcg agaggttgtg cacggcccct
     1921 ccgctcgcca agacgagtac gccctcgcgc cgcagcggtt cgagcgcgcg cccgacgcgg
     1981 acgtgcgccg ccgggcctgc gtgcggctgt accgaaagct gcgtcacggg gatgtccgca
     2041 gcaggataca tcagcgacag cggaacccaa gccccgtggt cgagtccgcg tccgggcgat
     2101 agctcgcaag agtgacccgc cgccttcaat agctcgcacg cgcgcgcggc cagctccggc
     2161 gcgcccggcg cgtcatacct catgcggtag agttcctccg gaaagccgcc gaagtcgtga
     2221 atcgtctccg gcctctccgc cgcgctcaag gtcggcacgc gcgtctccca atgcgccgag
     2281 acgcacagga tcgcccgcgg cctccccaac tcttcgccca gccccttcaa aaactcgacc
     2341 gtctcgttcc gctcgattat caaagtcggc gcgccgtgcg acacgaacac gctcggaaaa
     2401 acttcaacgc ccatccagcc cttcccttcc ctgcccgctc ttctcccgcc gccgcgacgg
     2461 ccccccgcgt ctgtcgttat aacgactatc aacgcaaaat tttttcactc cggcctgtcg
     2521 cgcgccgcgt cgagcagctt gctcagtgcc gcgaggcgct tctcgccgag gtgcgacagt
     2581 tggcgccggt gacactgcgc gaccgggcgg tcgagttcgg cgaggatgcg cagcccctct
     2641 tcggtgatgc gcgccgtgat gcggcgacga tccttcgcct cgcgcccgcg cgagatcagc
     2701 ccgcgcgctt cgagcctgtc cagcagccgc gtgatgtccg agtccttcgt caccagccgc
     2761 tcgctgatct cgccgcacga cgccccggcc tcgcccgccc cgcgcaggat gcgcagcacg
     2821 ttgtactgcg tcggcgtcag gtcggcccgc ttcagcgtct cgttgacgcc ccaccacagc
     2881 gcctccgccg tgcgctggat gttgaggtag acctcctcct cccggctggc gaacggcttc
     2941 gtcatcttca actctttccc cagattattc cccacgcgga taatatagtc gtcatgacga
     3001 ctatcgtcaa gacggggagg agaaaagtag gcagcaggca gcaggcagca ggcagcaggc
     3061 agcaggcagt aggcagtagg cagccaaagc aagaggcttg ggaggtaaag tccggtgcct
     3121 cctgtcttac tgcttgaagg gcggagaggc ttgggacttt acctctcaag gctcctcgcc
     3181 atccctcttg actgcctact gcctgctgcc tgctgcctac tctttacgta tcgggtgcgc
     3241 ctcggtgttg aagacgacgg cgtcgaagtc gagcgtgcgg ggcggggcga agaggaggta
     3301 gaagtatttg aaggtttcgg cgaagaggaa gctctgcatc gagtcggact tctccttcgt
     3361 gacgacgctt ttgagcgcgg cgtacgcctc gtccgtgcgg cagtgtttga cgaagtcacg
     3421 gaagagcgtc tcgcccatcc gcaggtagcg cgggtcgcgc gtgtagcgaa acaggtagta
     3481 ggtggattcg acgatctcgg gcc
//
LOCUS       DBNGOH010000825         1797 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_672813, whole genome shotgun sequence.
ACCESSION   DBNGOH010000825 DBNGOH010000000
VERSION     DBNGOH010000825.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1797)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1797)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1797
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_672813"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..373)
                     /locus_tag="AC159P_15940"
     CDS             complement(<1..373)
                     /locus_tag="AC159P_15940"
                     /inference="COORDINATES: protein motif:HMM:NF024668.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HIR3696988.1"
                     /translation="MPSRRACQPPRRPRRRGAPRRVPLSRARRRIPGARKSPLVARKP
                     SLDTGRKLRIMWARGGRPRRAPRVLKPQRAPGLCRPKLGPRGRPEEAMKIYISSTFKD
                     LKEYRAAAYSQLRQMRHDPLSM"
     gene            424..1695
                     /locus_tag="AC159P_15945"
     CDS             424..1695
                     /locus_tag="AC159P_15945"
                     /inference="COORDINATES: protein motif:HMM:NF014164.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HIR3696989.1"
                     /translation="MIRKLLPLSCVAAAAGLFASACAPAPPAANTTANTAPAANASAS
                     PSAQAAAKKAPTDLEQLAERLVTQSAAVKEGELVLISGGAQDLELLENIAVHVRKVGA
                     FPLIDIGSDRLTKRMFFDVPEKYDAQPRTLGMKIAENFNVTISVDSNLTEGLLADADP
                     KRRAARGKAGEPVAEAFLKRNVRTVEVGNGLYPTAWRAARYKLSEGELSKTFWEGVNV
                     DYASLAARAEEVRGVVAAGKEVQIKDANGTDLRVKVEGRPVLTSDGIISAEDARKGGA
                     AVSVYLPAGEVFVSPVAGTAEGKIVRPREVFEGKEVQNLTLTFAGGKLTSMTGTGPGF
                     DAMKAQYDAAGEGKDLFAVVDLGINPNVKLPADASVGTWVPAGTVTVGMGNNVWAGGD
                     NKTSYAYFVSLTGTTLTLDGKPIVENGQLKI"
BASE COUNT          315 a          628 c          594 g          260 t
ORIGIN      
        1 ccatcgagag cgggtcgtgg cgcatctggc ggagctggct gtaggcggcg gcgcggtact
       61 ccttgaggtc tttgaacgtc gaagagatgt agattttcat cgcttcctcg gggcggcctc
      121 gcgggccgag cttggggcgg caaagtcctg gagcgcgttg cggcttcaac acgcgcgggg
      181 cgcggcgcgg acgaccgccg cgcgcccaca tgatacgcag ttttcgtccc gtatcaagac
      241 tcggcttgcg tgcaacgagg ggcgacttgc gcgcgcccgg aatccgccgg cgcgcgcgag
      301 agagcggtac gcgtcggggc gcgccgcgtc gccgcggtcg ccgcggcggt tgacacgcgc
      361 gccgcgatgg catatgaagc gccctgttct tccatccccc tcgccgcgaa ggagtctcga
      421 aacatgatcc gtaaactctt gcccctctcc tgcgtcgccg ccgccgccgg actcttcgcc
      481 tcggcctgcg cgcccgcgcc gcccgccgcg aacacgaccg cgaacaccgc gcccgcggcc
      541 aacgcctcgg ccagcccgtc cgcgcaggcc gccgcaaaga aggcgccgac cgacctcgaa
      601 caactcgccg agcgcctcgt cacgcagagc gccgccgtca aggagggcga actcgtcctc
      661 atctccggcg gcgcgcaaga ccttgagctg ctggagaaca tcgccgtcca cgtccgcaag
      721 gtcggcgcct tcccgctcat cgacatcggc agcgaccggc tgacgaagcg catgttcttc
      781 gacgtgcccg aaaagtacga cgcgcagccg cgcacgctcg gcatgaagat cgccgagaac
      841 ttcaacgtca cgatcagcgt ggacagcaac ctcacagagg ggctgctcgc cgacgccgac
      901 ccgaagcggc gcgcggcgcg cggcaaggcg ggcgaacccg tcgccgaagc cttcctcaag
      961 cgcaacgtcc gcacggtcga ggtcggcaac ggtctctacc cgaccgcgtg gcgcgcggcg
     1021 cgctacaagc tgagcgaggg cgagctgtcg aagactttct gggagggcgt caacgtcgat
     1081 tacgcgagcc tcgccgcgcg cgccgaagag gtgcgcggcg tggtcgccgc cgggaaggaa
     1141 gtccagatca aggatgcgaa cggcacggac ctgcgcgtga aggtcgaggg gcggcccgtg
     1201 ctcacgagcg acggcatcat ctcggcggag gacgcgcgca agggcggcgc cgccgtcagc
     1261 gtctacctgc ccgcgggcga ggtcttcgtc tcgcccgtcg ccggcacggc ggaagggaag
     1321 atcgtccggc cccgcgaggt cttcgagggc aaggaggtac agaacctgac cctcacgttc
     1381 gcgggcggca agctcacttc gatgaccggc acggggccgg gcttcgacgc gatgaaggcg
     1441 cagtacgacg ccgcgggcga aggcaaagac ctcttcgccg tcgtcgatct cggcatcaac
     1501 ccgaacgtca agctccccgc cgacgcctcg gtcggcacgt gggttcccgc gggcaccgtc
     1561 accgtcggca tgggcaacaa cgtctgggcc ggcggcgaca acaagacctc ctacgcctac
     1621 ttcgtctcgc tcaccggcac caccctcacc ctcgacggca agcccatcgt cgagaacggc
     1681 caactcaaaa tctaaaagcc gtaaaccgta aatcgtgaac cgtgacaagt ggaaagccgg
     1741 ttcttactta tcacggttta cggtttacgg ttcacggctt ttgaggcgtt ggctctt
//
LOCUS       DBNGOH010000826         9919 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_307709, whole genome shotgun sequence.
ACCESSION   DBNGOH010000826 DBNGOH010000000
VERSION     DBNGOH010000826.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 9919)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9919)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9919
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_307709"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(45..947)
                     /locus_tag="AC159P_15950"
     CDS             complement(45..947)
                     /locus_tag="AC159P_15950"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol/lipid kinase family protein"
                     /protein_id="HIR3696990.1"
                     /translation="MTEKRSAVLISNPNAGQGGAAERAREVIRFCEGVESRGMVVEVW
                     QTAGPNDAARLAAEAVTAGAREVVVRGGDGTLHEAIQGLAGKGVRVAVWPAGTANVLA
                     RQLALPFDAERAAAVLARGRTKRISLGCATDERTGARRYFFMMAGIGLDASVVRRVRP
                     RLKRRVGEVAYWYSGLSHLAHWRPQEFTVEVGGESFPATYAAIGKAPWYGGGLAITPR
                     ARLDADEFEVCVINTHSRLRYLRLLAHAMRGGAPEGTRAVKFVRASRVRASGTADVQA
                     DGELIGELPMTFEIVNDGLELFVP"
     gene            complement(1186..2319)
                     /locus_tag="AC159P_15955"
     CDS             complement(1186..2319)
                     /locus_tag="AC159P_15955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015243843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009247 - glycolipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGDG synthase family glycosyltransferase"
                     /protein_id="HIR3696991.1"
                     /translation="MLNNVLILSASAGAGHLRAAAAVERAVEEAGAAEEVRHVDTLEY
                     TNKVFRHLYSKAYIEMVNKTPELLGWLYDALDKPWKNERRRLALDKLNTRPFIKMLKE
                     YRPDAVVCTHFLPAEIISWLRAKRRINTKQVIVVTDFDLHAMWLCHHYEHYFVALEET
                     RVHMERLGIPPEKISVTGIPIDPVFALDKDRHAMREKHGLRQDAATILLSAGGFGVGP
                     VEKIVRLLSELKNAAEVVALCGRNDELRARVERAAAEVRARAPHVTVKAVGYTTEMDE
                     YMSAANILLGKPGGLTTSEALAKGLVFCVVNPIPGQEERNSDHLLEEGVAVRCNNLPA
                     LAYKLDLLLGDDARLGAMRYNALRLARPHAARHVVEKLLSLVN"
     gene            complement(2434..3900)
                     /gene="guaB"
                     /locus_tag="AC159P_15960"
     CDS             complement(2434..3900)
                     /gene="guaB"
                     /locus_tag="AC159P_15960"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HIR3696992.1"
                     /translation="MLTPDMEEGLTFDDVLLVPARSDVLPTETDTATQFTRTIRLQIP
                     LCSAAMDTVTESRLAIAIAQQGGIGVVHKNFPIERQAEEVDRVKRSESGMIVDPVTIR
                     YDRPVRDALAVMERYKISGVPVVDEDGHLVGIITNRDLRFETRFDIPVSEVMTPQPLV
                     TVPVGTTLEEAKGVLQKHRIEKLLVIDTDKHLKGLITVKDIQKAIRYPNAAKDDLGRL
                     RVAAAIGATGDYRERADELVKARADCLVIDTAHGHSTRVIEAVRDIKRRHPETQLIAG
                     NVATGEAARELISAGVDAVKVGIGPGSICTTRVVTGAGVPQITAITSCVRAAHGSGVP
                     VIADGGVKFSGDVAKAIAAGADVVMIGSLFAGTEESPGELILFQGRSFKAYRGMGSIG
                     AMREGSRDRYAQEATEVESKLVPEGIEGRVPYKGTLADMVTQLVGGLRAGMGYTGCHN
                     IREFQDKTRFVRISSAGLKESHVHDVIITKEAPNYRLE"
     gene            complement(4047..4871)
                     /locus_tag="AC159P_15965"
     CDS             complement(4047..4871)
                     /locus_tag="AC159P_15965"
                     /inference="COORDINATES: protein motif:HMM:NF024805.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR3696993.1"
                     /translation="MNPKTSDQTLDGGTRAGEADGSLGQQLSRAREARGISLRDISEQ
                     TRITMRHLEAIESSDYKHLPGGIFNRSFIKAYARLIDFPEQRALDLYAREARERGETE
                     EVATSPRRSHVYTGDVSRSPAMTALLTAIVLGLLVLIVYAGLHWYRRTGDDANANAPA
                     ANANAATGAPAQPAAQTQPPAADEAGFRIQLKAKDRGFWLLTRADEAKEKGRILNLDK
                     PEDLEPQDRLYLRMDKASAGFLEVTVNGRPLKTPPAGAGNEVEWTITKENYRQFLP"
     gene            5107..5373
                     /locus_tag="AC159P_15970"
                     /pseudo
     CDS             5107..5373
                     /locus_tag="AC159P_15970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183618.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
     gene            5592..7511
                     /locus_tag="AC159P_15975"
     CDS             5592..7511
                     /locus_tag="AC159P_15975"
                     /inference="COORDINATES: protein motif:HMM:NF012398.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIR3696994.1"
                     /translation="MAVDKDLVSVQQARDLVEAAHRAQGLLARFDQGKIDRVCEAMAR
                     AALAEAARLGALAVEETGYGVADDKREKNRFAAEDVWNFFKSLRTVGVINESQDVLEI
                     ASPRGVVAGIIPSTNPTSTAIFKILIAIKSRNAIVLSPHPSAAKCINETARVMREAGL
                     KEGLPADAVACMTSATIEGTETLMKHKQTAVILATGGIGLVRAAYSSGKPAFGVGPGN
                     VPVFIERTADVPLAVQNILTGKCFDNGTICASEQAIVADAPVAKQVRQELEAQGAHFL
                     SASEADQLAKVVATPQRTLNPGIVGRNVEVIAKMAGITLPPGTRCMVAEVGGVGREHP
                     LSMEKLSPILAFYVEDGHERGAARCFEILSYGGMGHTAGIHTRSRDMARVFGTEMPAS
                     RVVVNTPTTHGAIGFSTALPPSMTLGCGSWGGNVTSDNISPWHLMDIKRVAFETRPVP
                     SKRPALARQGDTATRGLGAQITPSPRHPITPSPAVGVSRDEIARIVDRFLTGRQTTQP
                     APTPTAAPLHAAQSPAPVQADIDIEPQRVSPSGGVAPQPEAAPTVEAGTGNGAQSTTT
                     ATAAPPPAPQANGKQDAPQTPSPANGKRAVDFVCEDDVRRAIQKGEKIYTNARTIITP
                     AARDMGEAAEVFAKA"
     gene            7604..7942
                     /locus_tag="AC159P_15980"
     CDS             7604..7942
                     /locus_tag="AC159P_15980"
                     /inference="COORDINATES: protein motif:HMM:NF015289.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HIR3696995.1"
                     /translation="MFLGKVVGTVWSTKKTPDLEGVRFLIVHPIDLVKEPTRAVVVVA
                     DRLGAGVGEIVMCAYGKAARSAVGDQEMSIEAAVVGIVDRVDVQEDIARSDELVEEAR
                     ELLRARDNGR"
     gene            8038..8505
                     /locus_tag="AC159P_15985"
     CDS             8038..8505
                     /locus_tag="AC159P_15985"
                     /inference="COORDINATES: protein motif:HMM:NF001220.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HIR3696996.1"
                     /translation="MPNEELIYRITRAVYGRLGERADEREVENLVMDVYRAIEPVVEA
                     GGGAGVGAAAPSSMMGGGEGRADRLVVSVFGVDHPGIVAAVSQILAEAGCSIVDINQT
                     VVQGKFAMVIVADISRASETAAALKERFRREGERLGVRIYAQREDLFNAMHRI"
     gene            8635..9297
                     /locus_tag="AC159P_15990"
     CDS             8635..9297
                     /locus_tag="AC159P_15990"
                     /inference="COORDINATES: protein motif:HMM:NF024637.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HIR3696997.1"
                     /translation="MQPEISRRCLVCGASVRAGAQFCSQCGRRLGERGGAGAESSDVR
                     PDARAGEAPSADAAGGGEVERAFETWRSAEHVAAEEARRTRVAEPAAGDDVRPATADD
                     SARPAAAGGNARAPVAGVAEPPPPTREAVVPETRALEPPPPRDEGATGARLAGAVGAT
                     RKRAASVVKDAITPRVERVRDRSIVMLEEAPDDSGLRFVLIAAALFALFIFLLVLNTV
                     IK"
     gene            9325..>9919
                     /locus_tag="AC159P_15995"
     CDS             9325..>9919
                     /locus_tag="AC159P_15995"
                     /inference="COORDINATES: protein motif:HMM:NF017019.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF711 family protein"
                     /protein_id="HIR3696998.1"
                     /translation="MDYTQAEILQTVRMTEMEHLDVRTVTLGISLRDCTRDTAEATAE
                     ASRAKIVRVGGRLVPVVNEIGEEFGLRVANKRVSITPVSLVMGGAARNASDCVRVARA
                     IDDAAREVGVDFIGGYSALVHKGMTETESIFLDSIPEALATTERLCSSLNVATTRAGI
                     NMDAVRRVGLLVKDAAERTRERGGLACAKFVCFANAVE"
BASE COUNT         1613 a         3280 c         3419 g         1607 t
ORIGIN      
        1 actttcaagc acttccgcct cacgtccaca ccctcacgct cccttcaagg cacgaagagt
       61 tcgaggccgt cgttgacgat ctcgaaggtc atcgggagtt cgccgatcag ctcgccgtcg
      121 gcctgcacgt cagccgtgcc cgaggcgcgc acgcgcgagg cgcggacgaa cttgacggcg
      181 cgcgtgcctt cgggcgcgcc gccgcgcatg gcgtgggcga gcagccgcag gtagcgcagg
      241 cggctgtgcg tgttgatgac gcagacctcg aactcgtccg cgtcgaggcg cgcgcgcggc
      301 gtgatggcga ggccgccgcc gtaccacggc gccttgccga tggccgcgta ggtggcgggg
      361 aaactctcgc cgcccacctc gacggtgaac tcctgcgggc gccagtgcgc gaggtggctg
      421 aggcccgagt accagtaggc gacctcgccc acgcggcgct tcaggcgcgg acgcacgcgg
      481 cgcacgaccg acgcgtcgag gccgatgccc gccatcatga agaagtagcg ccgcgcgccc
      541 gtccgctcgt ccgtcgcgca gcccagagag atgcgcttcg tccgcccgcg cgcgagaacc
      601 gccgccgcgc gctcggcgtc gaagggcagt gccaattgcc gcgccagcac gttcgcagtc
      661 cccgcgggcc acaccgccac gcgcacgcct ttgcccgcga gaccctgtat cgcctcgtgc
      721 agagtcccgt cgccgccgcg cacgacgacc tcgcgcgcgc ccgccgtgac ggcttcggcg
      781 gccagacgcg ccgcgtcgtt cggccccgcc gtctgccaca cctcgacgac catgccgcgc
      841 gactcgacgc cctcgcagaa acgtatcacc tcgcgcgcgc gctcagccgc gccgccctgc
      901 cccgcgttcg ggttcgagat gagaaccgcc gatctttttt ccgtcatcac tgttgccgtg
      961 tcgaagcgcg cgacgaccgc ccgtcactac ggttcgcaaa ctgctctgca aaacgggtgc
     1021 cgccgccgca ggacgagaaa gcgcgcggcc cgcgtcggcg ctggggcgct cccgcgcgca
     1081 cgagcagccg gacacgtgtc ggaggaatta tgcgcggggg gcgcgcaagg gaactcgcgc
     1141 ctctcggttt accggcgggg cgcgcgaagg tttctcgcgg acgtttcagt tgaccagcga
     1201 gagaagtttc tcgacgacgt ggcgcgccgc gtgcgggcgc gcgagccgga gcgcgttgta
     1261 gcgcatcgcg cccaagcgcg cgtcgtcgcc gagcagcaaa tcaagcttgt atgcgagtgc
     1321 gggcaggttg ttgcagcgca cggcgacgcc ttcttccaaa agatgatcgg agttgcgctc
     1381 ctcctgtccg gggatcgggt tgacgacgca gaagacgaga cccttggcga gcgcctcgga
     1441 ggtcgtcagt ccgcccggct tgccgagcag gatgttggcg gcgctcatat actcgtccat
     1501 ctccgtcgtg taacccacgg ctttgaccgt gacgtgtggc gcccgcgcac gcacttcggc
     1561 ggcggcgcgc tcgacgcgcg cgcgcagctc gtcgttgcga ccgcagagcg cgacgacctc
     1621 agccgcgttc ttcaactccg agaggaggcg cacgattttc tcgaccgggc cgacgccgaa
     1681 gccgcccgcg gagagcagga tcgtggccgc gtcctgacga aggccgtgct tctcgcgcat
     1741 cgcgtgccta tccttgtcga gggcgaacac ggggtcgatg gggatgccgg tgacggaaat
     1801 cttctcgggc gggatgccga gccgctccat gtgcacgcgc gtctcttcga gcgcgacgaa
     1861 gtagtgctcg tagtggtggc acagccacat cgcgtggagg tcgaagtcgg tgacgacgat
     1921 gacctgtttg gtgttgatgc gccgcttcgc gcgcagccac gagatgatct cggcgggcag
     1981 gaagtgcgtg cagacgaccg cgtccggccg gtactccttc agcatcttga tgaagggccg
     2041 cgtgttcagc ttgtcgagcg cgagccgccg ccgctcgttc ttccacggct tatcgagcgc
     2101 gtcgtacagc caccccagca gctccggcgt cttgttcacc atctcgatgt acgccttcga
     2161 gtacaggtgc cggaacacct tgttcgtgta ctccagcgta tcgacgtgcc gcacctcctc
     2221 tgccgcgccc gcctcctcga cggcgcgctc caccgccgcc gccgcacgca ggtgccccgc
     2281 gcccgccgag gccgagagga tgagaacgtt gttaagcatc gtgaaaagtg aaaggcaaag
     2341 ctcaggatga caaagtcaga aggcagaacg cagaaggatg aatgaaagac aaattgcttt
     2401 ttattcatcc ttctgcgttc tgccttctga tttctattcc agccggtagt tcggcgcttc
     2461 cttcgtgatg atgacgtcgt ggacgtgcga ctctttgagg ccggctgagc tgatgcggac
     2521 gaagcgggtc ttgtcctgaa attcgcggat gttgtgacag ccggtgtagc ccatgcctgc
     2581 gcggaggccg ccgacgagct gcgtgaccat gtcggcgagc gtgcctttgt aggggacgcg
     2641 gccctcgatg ccttcgggga cgagcttgga ctcgacctcg gtcgcttcct gcgcgtagcg
     2701 gtcgcgcgag ccttcgcgca tggcgccgat gctgcccatg ccgcggtagg ctttgaagga
     2761 gcggccctgg aaaagaatca gctcgccggg cgactcttcg gtgcccgcga agagcgagcc
     2821 gatcatcacg acgtcggcgc ccgcggcgat ggccttcgcc acgtcgccgg agaacttcac
     2881 gccgccgtcg gcgatgaccg gcacgcccga gccgtgcgcg gcccgcacgc agctcgtgat
     2941 ggccgtgatc tgcgggacgc ccgcgccggt gacgacgcgc gtcgtgcaga tcgagccggg
     3001 gccgatgccg accttgaccg catcgacgcc cgccgagatc agctcgcgcg cggcctcgcc
     3061 ggtggcgacg ttgcccgcga tgagctgcgt ctcggggtgg cggcgcttga tgtcgcgcac
     3121 cgcctcgatg acgcgcgtgg agtggccgtg cgcggtgtcg atgacaaggc agtcggcgcg
     3181 cgccttgacc agctcgtccg cccgctcgcg gtagtcgccg gtggcgccga tggcggcggc
     3241 cacgcgcagg cgcccgaggt cgtccttcgc ggcgttcggg tagcggatgg ccttctggat
     3301 gtccttgacc gtgatgaggc ccttgaggtg cttgtccgtg tcgatgacga gcagcttctc
     3361 gatgcggtgc ttttgcagca cgcccttcgc ctcttcgagc gtggtgccca cgggcacggt
     3421 gaccaatggc tgcggcgtca tcacctcgga gacggggatg tcgaagcgcg tctcgaagcg
     3481 gaggtcgcgg ttggtgatga tgccgacgag atggccgtcc tcgtcaacga ccggcacgcc
     3541 gctgatcttg tagcgctcca tcacggcgag cgcgtcgcgg acggggcggt cgtagcggat
     3601 cgtcaccggg tctacgatca tgcccgactc ggagcgcttg acgcggtcaa cctcttcggc
     3661 ctgccgctcg atggggaagt ttttatggac gacgccgatg ccgccctgct gcgcgatggc
     3721 gatggcgagg cggctctcgg tgacggtgtc catcgccgcc gagcacaagg ggatttgcag
     3781 gcggatggtg cgcgtgaact gcgtcgcggt gtcggtctcg gtcggcagca cgtcggagcg
     3841 cgcggggacg agcagcacgt cgtcgaaggt cagaccttct tccatgtcag gggtcagcat
     3901 gattcacaac ctcggttcga tagctcgggg ttcgaagttc aaggtctcgc ggcgccgaaa
     3961 gtcgcgcgta taaaaagagg cccgaagttc agagcgagac cgcgtagtcg gactatgaac
     4021 ttcgaacctg ggactttgaa ctcgcattaa ggcagaaact gtctgtagtt ttctttcgtg
     4081 atcgtccact cgacctcgtt gccggcgccc gcgggcggcg tcttgagggg ccgaccgttg
     4141 accgtcactt cgagaaagcc cgcggaagcc ttgtccatcc gcaggtagag tctgtcctgc
     4201 ggctccaggt cttccggctt gtcgaggttg aggatgcgcc ccttctcctt cgcctcgtcg
     4261 gcgcgcgtca gcagccagaa gccccggtct ttggccttca actgaatcct gaagccggcc
     4321 tcgtccgccg cgggcggctg cgtctgcgcg gcgggctgcg ccggtgcgcc cgtggcggcg
     4381 ttcgcgttgg cggcgggcgc gttcgcgttc gcgtcgtcgc cggtgcgcct gtaccagtgc
     4441 agccccgcgt agacgatgag cacgagcagg ccgaggacga tggccgtcag cagcgcggtc
     4501 atcgcgggcg agcgcgacac gtcgccggta tagacgtgcg agcggcgcgg gctggtcgcc
     4561 acctcttccg tctcgccgcg ctcgcgcgcc tcgcgggcgt agaggtcgag cgcgcgctgt
     4621 tccggaaagt cgatcagtct ggcgtaggct ttgatgaagc tgcggttgaa gatgccgccg
     4681 ggcaggtgct tgtagtcgct cgactcgatg gcttcgaggt ggcgcatcgt gatgcgcgtc
     4741 tgctccgaga tgtcgcgcag ggagatgccg cgcgcctcgc gcgcccggct caactgctgg
     4801 ccgagagagc cgtccgcctc cccggcccgc gtaccgccgt cgagagtctg gtcggaggtc
     4861 ttagggttca cgtggcgcgt aatctcaacc gaattattga caccggctcg aaggtggggc
     4921 gggcagccgc cccgccgctt gcgcaaacta taatgaaagc ctgcgagcag tgtcaaacaa
     4981 cgtctatcgc gcgcgaggcg cgcgtcttga actttgccgc gcgcggtgct acggtgtggg
     5041 ccaaccgagg acagcagcac gatcagcttt taacttctat aaccagaaaa gcgaggagtt
     5101 ccacagatgg ctctcgaagc actcggaatg gtggagacca aggggttcgt cggcgccgtc
     5161 gaggccgcgg acgcgatggt caaagccgcc aacgtacagc tcctgggcaa ggagtacatc
     5221 ggcgccggct atgtcacgat cttcgtgcgc ggcgacgtgg gagcggtcaa ggccgcgacc
     5281 gacgccgggg ccgccgccgc acgcagagtc ggcgaactca tcagcgtcca cgtcatcccc
     5341 cgcccccacg ccgaagtcga acgcgtcctg cccgccaacg gtcgcaccgg ctacagcccc
     5401 gacgagcgcg ccattcaggg cggggcgacg cgcggcgaac tcagcgaagg cgaacagggc
     5461 cgctcgctgc aaggcgcgcg cgagaacaac aaagagcgta acaagtaaac cgtaaaccgt
     5521 gacaagtcag aaatccgttc ctctcttgtc acggttcacg gttgacggtt cacggctttc
     5581 ggaggttcgt catggcggtt gacaaagacc tcgtatcggt gcagcaggcg cgtgacctcg
     5641 tcgaggcggc gcaccgcgcg caagggcttt tggcgcggtt cgatcagggg aagatcgacc
     5701 gcgtgtgcga ggcgatggcg cgggcggcgc tggcggaggc ggcgcggctc ggcgcgctgg
     5761 cggtcgagga gacgggctac ggcgtcgccg acgacaagcg cgagaagaac cgcttcgccg
     5821 ccgaggacgt gtggaacttc ttcaaatctt tacgcacggt cggcgtcatc aacgagtcgc
     5881 aggacgtgct ggagatcgcc agcccgcgcg gcgtcgtcgc cggcatcatc ccttcgacca
     5941 acccgacctc caccgccatc ttcaaaatcc tcatcgccat caagtcgcgc aacgccatcg
     6001 tcctctcgcc gcacccctcc gccgccaagt gcatcaacga gacggcgcgc gtcatgcgtg
     6061 aagcgggatt gaaagaagga ctaccggcgg acgcggtcgc gtgcatgacc tcggcgacga
     6121 tcgaaggcac cgagacgttg atgaagcaca agcagaccgc cgtcattctg gcgacgggcg
     6181 gcatcggcct ggtgcgcgcg gcctactctt cgggcaagcc cgccttcggg gtcggccccg
     6241 gcaacgtgcc cgtcttcatc gagcggacgg cggacgtgcc gctcgccgtg cagaatattt
     6301 tgacgggcaa gtgcttcgac aacggcacca tctgcgcgtc cgagcaggcg attgtcgccg
     6361 acgccccggt ggcgaagcag gtgcggcagg aactcgaagc gcagggcgcg cacttcctct
     6421 ccgcctcgga agcggaccag ctcgccaaag tcgtcgccac gccgcagcgc actttgaacc
     6481 ccggcatcgt aggccgcaac gtcgaagtga tcgcgaagat ggcgggcatc accttgccgc
     6541 cgggcacgcg ctgcatggtc gcggaggtgg gcggcgtcgg cagagagcac ccgctctcga
     6601 tggagaagct gtcgccgatt ctcgccttct acgtcgagga cgggcacgag cgcggcgccg
     6661 cgcgctgttt cgaaatcctg agctacggcg gcatgggcca caccgccggc atccacacgc
     6721 gctcgcgcga catggcgcgc gtcttcggca ccgagatgcc cgcctcgcgc gtggtcgtca
     6781 acacgccgac cacgcacggc gccatcggct tctcgacggc gctcccgcct tcgatgactt
     6841 tggggtgcgg ctcgtggggc ggcaacgtca cctccgacaa catctcgccg tggcacctga
     6901 tggacatcaa gcgcgtcgcc ttcgagacgc gccccgtccc gagcaagcgg cccgccctcg
     6961 cgcgacaggg ggacacagcg acaaggggac tgggcgcaca aatcaccccg tcaccccgtc
     7021 accccatcac cccatcaccg gcggtcgggg tcagccgcga cgagatcgcc aggatcgttg
     7081 accgcttcct gacgggacgg cagacgactc aacccgcgcc gaccccgacc gcggcgccgc
     7141 tccacgccgc gcagtcgccc gcgcccgtgc aggccgacat agacatcgag ccgcaacgcg
     7201 tcagcccttc gggcggcgtc gccccgcagc ccgaggccgc cccgaccgtc gaagccggca
     7261 ccggcaacgg cgcgcaatcg acgacgaccg cgacagccgc gccgccgccc gcgccgcaag
     7321 cgaacggcaa gcaggacgcg ccgcaaacac cgtcgcccgc caacggcaag cgcgccgtcg
     7381 atttcgtctg cgaagacgac gtgcgccgcg ccatccagaa gggcgagaag atttacacca
     7441 acgcgcgcac catcatcact cccgccgcac gcgacatggg cgaggccgcg gaagtgttcg
     7501 ccaaagcata gctgctagct gttagcgatt agctgttagc taacaactgt cggcaaactg
     7561 tttccagcta atcgctaaca gctagcagct aatcgcttct tttatgtttc taggaaaagt
     7621 cgtcggcacg gtctggtcaa cgaagaagac gcccgacctt gagggcgtcc gttttttgat
     7681 cgtgcacccg atagatttgg tcaaggagcc gacgcgcgcc gtcgtcgtcg tcgcggatcg
     7741 actcggcgcg ggcgtgggcg aaatcgtgat gtgcgcttac ggcaaggccg cgcgctcggc
     7801 ggtcggcgat caggagatgt cgatcgaggc ggcggtcgtc ggcatcgtgg atcgcgtgga
     7861 cgtgcaggag gacatcgcgc gctcggacga actggtcgag gaggcgcgcg agctgctgcg
     7921 ggcgcgggac aacgggaggt gaaaaagaag tgatgaatga tgagtgcaga atgatgaatg
     7981 aaagacaaag tgcttttaat tcatcattct gcactcatca ttcatcactt cgtggcgatg
     8041 cctaacgaag agctgattta tcgcatcacg cgcgccgtct acgggcggtt gggcgagcgc
     8101 gcggacgagc gcgaggtgga gaacctcgtg atggacgtgt accgcgcgat cgagccggtg
     8161 gtcgaggcgg gcggcggcgc gggcgtgggc gcggcggcgc cgtcgtcgat gatggggggc
     8221 ggcgaggggc gcgcggatcg gctcgtcgtc tcggtcttcg gcgttgacca tccgggcatc
     8281 gtcgccgccg tctcgcagat tctggccgag gccgggtgca gcatcgtgga catcaaccag
     8341 acggtggtgc agggcaagtt cgcgatggtc atcgtcgccg acatctcgcg cgcgagcgag
     8401 acggcagccg cgctgaagga gcgcttccgc cgcgagggcg agcgcctcgg cgtccgcatc
     8461 tacgcgcagc gcgaagacct cttcaacgcg atgcatcgaa tctaaaagcc gtgaaccgtg
     8521 aaccgtaaac cgtgacgagt taaaagcagc atccactttg ccgctcgtcg cccgtcattc
     8581 gtcaccgctt ttcacttgtc acggttcacg gttcacggtt cacggtcttt aattatgcag
     8641 ccggaaatct cacgtcggtg tctggtgtgc ggcgcgtccg tgcgcgccgg ggcgcagttc
     8701 tgttcgcagt gcgggaggag gctgggcgag agaggcgggg cgggcgccga gagttcggac
     8761 gtgcggcccg acgcgcgtgc gggcgaagcg ccgtcggcgg acgccgcggg cgggggcgaa
     8821 gtcgaacgcg cgttcgagac gtggcggagc gcggagcacg tcgcggctga agaggcgcgg
     8881 cgcacgcgcg tcgctgagcc tgcggcgggc gacgacgtgc gccccgcgac ggctgacgac
     8941 tcagcccgcc cggcggcggc gggcggcaac gcgcgcgcac ccgtcgcggg cgtggcggag
     9001 ccgcctcctc ctacgcgcga ggcggtggtg cccgagacgc gcgcgttgga gccgcccccg
     9061 ccgcgggatg agggggcgac gggggcgagg ttggccggcg cggtcggggc gacgcgcaag
     9121 cgcgcggcgt cggtcgtgaa ggacgccatc acgccgcggg tcgagcgcgt gcgcgaccgc
     9181 tccatcgtca tgctcgaaga ggcgccggac gattcgggcc tgcgcttcgt cctcatcgcc
     9241 gccgcgctct tcgcgctctt catcttcctc ctcgtcctga acaccgtcat caagtagcgc
     9301 cccggcgggc tggcacctcc ctttatggat tacacgcagg cggaaatctt gcagaccgtg
     9361 cggatgaccg agatggagca cctcgacgtg cgcacggtca cgctcggcat ctcgctgcgc
     9421 gactgcacgc gcgacacggc ggaggcgacc gccgaggcgt cgcgcgcgaa gatcgtgcgc
     9481 gtcggcgggc ggctcgtccc cgtggtcaac gagatcggcg aggagttcgg cctgcgcgtc
     9541 gccaacaagc gcgtctcgat cacgcccgtt tcgctcgtca tgggcggcgc ggcgcgcaac
     9601 gcgagcgact gcgtgagggt cgcgcgcgcc atcgacgacg cggcgcgcga ggtcggcgtg
     9661 gacttcatcg gcggctactc ggcgctcgtc cacaagggga tgaccgagac cgaaagcatc
     9721 ttcctcgatt cgatcccgga agcattggcg acgaccgaac gcctgtgctc ctcgctcaac
     9781 gtggcgacga cgcgcgccgg catcaacatg gacgcggtgc gccgcgtcgg cctgctcgtg
     9841 aaggacgcgg cggagcgcac gcgcgaacgc ggcggcctcg cgtgcgcgaa gttcgtctgc
     9901 ttcgccaacg cggtcgaag
//
LOCUS       DBNGOH010000827         3177 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1042099, whole genome shotgun sequence.
ACCESSION   DBNGOH010000827 DBNGOH010000000
VERSION     DBNGOH010000827.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3177)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3177)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3177
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1042099"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..612)
                     /locus_tag="AC159P_16000"
     CDS             complement(<1..612)
                     /locus_tag="AC159P_16000"
                     /inference="COORDINATES: protein motif:HMM:NF012698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HIR3696999.1"
                     /translation="MFGRFRLDAGKRLLSAGAEVVGLTPKAFDTLLALVDNRGRVVSK
                     EELMSRVWAGQFVEENNLAQHIHAIRRSLGDGAEGVKYIETVPKRGYRFVAEVEVQES
                     PAAATEAGGTARALSHAAGESTVAKSPTSSRGLAYAPAFAAGSRAAPAVPKTRYAMST
                     GGVNIAYQTLGDAPLDLVFVMGWVSHLEYFWREPRFARFLRRLA"
     gene            824..1291
                     /locus_tag="AC159P_16005"
     CDS             824..1291
                     /locus_tag="AC159P_16005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697000.1"
                     /translation="MTNLSKARSAGLACMLGGLLILYTFVVLNPPTEASSGQAAPYAF
                     PAVWTLALCVAAVCLAQALAGRFALETSDNGGRKAPKLSGGTTVRPRLRTSFLDKVFI
                     DNFPERAFQQLSPPGGPVLLTHDMFWPGWAAPPFHLTQRKPMPQMPQRTPTGS"
     gene            1648..2550
                     /locus_tag="AC159P_16010"
     CDS             1648..2550
                     /locus_tag="AC159P_16010"
                     /inference="COORDINATES: protein motif:HMM:NF024627.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HIR3697001.1"
                     /translation="MFGALALLACSLAFASPARAEVTHTVVSANGLDNRLCDRANPCR
                     TFDAAITAVEEGGEISVLSSGVYEPFTINKSVQIVAPAGVHALIAAKSGNAITVSIGP
                     ADTVVLRGLYLDGLRTASGGIRAQASPTTGTLHVENCVVSNFKGFGVYFSSGGGQLYV
                     KDTTARNNLSGIFITGNVRATVDHCRMEQNQLDGLSAQLGARVTVRDSVAAGNATEGD
                     LETGGFHTIDGFLNIENCVSSGNSNGIRAEGGVVHVSNTMLTDNRFGLVNAGGFGQLI
                     SYGNNRVRGNNVSNGNFTLNVGQR"
     gene            2578..>3177
                     /locus_tag="AC159P_16015"
     CDS             2578..>3177
                     /locus_tag="AC159P_16015"
                     /inference="COORDINATES: protein motif:HMM:NF012488.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HIR3697002.1"
                     /translation="MTISRRRFMATGGISAAAAAGLLAASGPGARVTSASERTDAPPD
                     WSFVRDQFDLARDYIHLSSFFLASHPRPVREAIERHRRAIDENPFLYVERQMFEMQGK
                     VRAAAAEYLGGKPEEVALTNSTTMGLAFVYQGLPVQAGQEFLTTAHDHYVHHEAVRLA
                     AERAGATVKKIPLYDHIDSVSEEDIVRRIRNAVRRRTRAV"
BASE COUNT          568 a         1056 c          998 g          555 t
ORIGIN      
        1 ggcgagccgc cgcaggaagc gcgcgaagcg cggctcgcgc cagaagtatt cgaggtgcga
       61 gacccagccc atcacgaaga cgaggtcgag cggcgcgtcg ccgagcgttt ggtaggcgat
      121 gttcacgcca cccgtgctca tggcgtaccg ggtcttcggc acggcgggcg cggcccgact
      181 cccggcggcg aaggcaggag cgtaggcaag accccgagag gatgttgggg attttgcgac
      241 ggttgactct ccggcggcgt ggctcaatgc gcgtgccgtc ccgccggcct cggtcgccgc
      301 cgccggactc tcctgcacct cgacctcagc gacgaagcgg tagccacgtt tcggaaccgt
      361 ctcgatatac ttgacgcctt cagccccgtc gccgagcgac ctgcggatcg cgtggatgtg
      421 ttgcgccagg ttgttctcct ccacgaactg cccggcccag acgcggctca tcaactcctc
      481 tttgctcact accctcccgc ggttatcgac cagggccagg agcgtgtcga aggctttcgg
      541 cgtcaggccg acgacctcgg cgccggcgga gagcaggcgt ttgcccgcat cgagccggaa
      601 ccgcccgaac acgtaaacgc catgaccctc acttctcata gagtcattca gaaaaatttc
      661 ataactctct caagacattt ggtgaggccc cgccttattg tctggggcta cggcgggtcg
      721 gccacttcat ccggattaat cggaggccga ttatacctga tacgacaccc gccgggaaga
      781 tttcgcccgt gaggaaaaac gagggcggaa aggggattct tcaatgacga acttatcgaa
      841 ggcgcgctca gccgggctgg cgtgcatgtt gggggggctg cttattttat acactttcgt
      901 cgtgttaaac ccgccgaccg aggcctcctc cggacaggca gcaccctacg cgtttccggc
      961 ggtctggacg ctcgccttgt gcgtcgccgc cgtgtgtttg gcgcaagccc tcgcggggcg
     1021 attcgcactt gaaacgtctg acaacggcgg gcgaaaagcc cccaaacttt ccggcgggac
     1081 aaccgtgcgg ccccggctcc gcacctcctt tctggacaag gttttcatcg acaacttccc
     1141 cgaaagggct ttccagcaac tttccccgcc gggcggcccc gtgctgctca cgcacgacat
     1201 gttctggcca ggatgggccg cccctccctt ccacttaacc caacgtaaac cgatgccaca
     1261 aatgccgcag cgcactccga cggggtcgtg aaacggccct tgaggaaacc cgtcgtgcga
     1321 ttactccgct cgtccggtcg ttcgcaaact tgaacgcctt tgcgccggcg acagacgggg
     1381 gactccgcac cgttataggg gaggcgcccg cgtcccgccg tgagggcagc cggcgcccgt
     1441 tcgttagacg cggcacgggt ggtctgcgcg cgtccagggc gttgcgcgat tcgtcggcgc
     1501 gcgtgacttt acgccccatt ccgcggacga gaacgcgggc agggaacaga tacacgttga
     1561 cgtgtgttcg cgcctcggca cgcacgacga catgcgatta ataaggaaag gcaacacctg
     1621 atgctatcta caaatcctct tcgtctaatg ttcggcgcgc tggcgctcct ggcctgctcg
     1681 ctcgctttcg cttcgccggc ccgggccgag gtcacgcaca cggtcgtctc cgccaacggc
     1741 ctggacaacc ggctctgtga ccgggcaaac ccgtgccgca ctttcgacgc ggcgatcact
     1801 gccgtcgagg agggcggcga aatctcggtg ctgagttcgg gcgtgtacga gccgttcacg
     1861 atcaacaaat cggtgcagat cgtcgcgcct gcgggcgtcc acgccttgat cgcggcgaag
     1921 tccggcaacg cgattacggt aagcatcggc ccggcggata cggtcgtctt gcgcggactg
     1981 tacctcgacg gtctgcggac ggcgtccggc ggtatccggg ctcaagcctc cccgaccacc
     2041 gggacgctgc acgtcgagaa ctgtgtggtc agcaacttta agggtttcgg cgtctacttc
     2101 tcgtccggcg gcggccagtt gtacgtcaag gacacgaccg cgcgtaacaa cctcagcggg
     2161 atttttatca cgggtaacgt ccgcgccacg gttgaccact gccgcatgga gcagaaccag
     2221 ttagacggtc tgtccgcaca gctcggcgcg cgggtcacgg tgcgcgactc ggtcgcggcg
     2281 ggcaatgcca ccgaagggga cctcgaaacg ggaggcttcc acaccatcga cggctttctg
     2341 aacatcgaga actgcgtctc ctccggcaac tctaacggca tccgcgcgga aggcggcgtc
     2401 gtccacgtct ccaacacgat gttgacggac aacaggttcg ggctggtcaa cgccggcggc
     2461 ttcgggcagc tcatctcgta cggcaataac cgtgtgcgcg gcaataacgt cagcaacggc
     2521 aacttcacct taaacgtcgg gcagcggtaa ggggcggcgg gagaaatgga ggacatcatg
     2581 actatcagcc gccgccgctt catggctacg ggcggaatct ccgccgccgc cgccgccgga
     2641 ctccttgccg cgtccggccc gggtgcgcgc gtcacgtccg cgtcggagcg caccgacgcc
     2701 ccgccggact ggtcgttcgt gcgcgaccag ttcgatctcg cgcgcgacta cattcacctc
     2761 agctcattct tcctcgcctc gcacccccgc ccggtgcgcg aggcgattga gaggcaccgg
     2821 cgggcgattg acgaaaaccc gttcctgtac gtggagcggc agatgttcga gatgcagggg
     2881 aaggtgcgtg cggcagccgc cgaatacctc ggcggcaagc cggaggaggt ggccctaacc
     2941 aacagcacca cgatggggct ggccttcgtc taccagggcc tgcccgttca ggccgggcag
     3001 gagtttctga ccacggcgca cgaccactac gtgcatcacg aagcggtccg cctcgccgcc
     3061 gaacgggccg gggcgacggt caagaagatt ccgctctacg accacatcga ctctgtctcc
     3121 gaagaagaca tcgtgcggcg gatcagaaac gccgtgcgcc gcaggacccg cgccgtc
//
LOCUS       DBNGOH010000828         1850 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3240406, whole genome shotgun sequence.
ACCESSION   DBNGOH010000828 DBNGOH010000000
VERSION     DBNGOH010000828.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1850)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1850)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1850
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3240406"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1591
                     /locus_tag="AC159P_16020"
     CDS             <1..1591
                     /locus_tag="AC159P_16020"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HIR3697003.1"
                     /translation="RGYGMFVHTGAPVTFDFGKTFDSHNAIYTGDENLDLFIFFGSPK
                     EILSEYTALTGRSPVPPLWSFGFWMSRITYKSEDEVRDVAAKLRQHRLPSDVIHLDTG
                     WFETDWRSNYEFSKTRFRDPARMIADLRKQGFRVSLWQYTYFTPKNELYGEIVSKGFH
                     VRNESGGPPFEDAVLDMSNPEAVKWYQAKLAGLLKMGVGAIKVDFGEGAPPNGLYASG
                     RTGLHEHNLYPLRYNKAVADITREVTGEDIIWARSAWAGSQRYPLHWGGDAENTDSAM
                     AATLRGGLSFGLSGFTYWSHDAGGFVRRAPRDLYRRWMAWGVLTSHTRAHGEPPREPW
                     EYDEALTEDFRRALGLRYSLMPYILAQAKDSSAKGFPLLRALFFEYPEDPTSWTIDDQ
                     YLFGSDLLVAPLFEEGATARRVYLPPGAWVDYQSGKTYRGAAWHRIEAGPIPVILLAR
                     DHSVIPHVRVAQSTDGIDWGAVELRVFSTDDAAATGLFTTPQGPVQTLRLVPRGGGAY
                     ALGEDPSGGRVRWQINRFATR"
     gene            1645..>1850
                     /locus_tag="AC159P_16025"
     CDS             1645..>1850
                     /locus_tag="AC159P_16025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020702838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697004.1"
                     /translation="MNLNRSLTTARCVAALLLAFVALGPRPATTSAQPPRMDTILYGA
                     SYYHEYMPVERLDKDVQMMKDAGI"
BASE COUNT          310 a          657 c          603 g          280 t
ORIGIN      
        1 ccgcggctac ggcatgttcg tccacaccgg cgcgcccgtc accttcgact tcggcaagac
       61 cttcgacagc cacaacgcca tctacaccgg cgacgagaac ctcgacctct ttatcttctt
      121 cggcagcccg aaagagatcc tctcggagta cacggcgctg accgggcgca gcccggtgcc
      181 gccgctctgg tccttcggct tctggatgag ccgcatcacc tacaaatcgg aagacgaggt
      241 gcgcgacgtg gccgcgaagc tccgccaaca ccgcctcccc tcggacgtga ttcacctcga
      301 caccggctgg ttcgagaccg actggcggag caactacgag ttctccaaaa cgcgcttccg
      361 cgacccggcg cgcatgatcg cagacctccg caagcagggc ttccgcgtga gcctctggca
      421 gtacacctac ttcacgccga agaacgagct gtacggcgag atcgtctcga agggttttca
      481 tgtcaggaac gagtcgggcg gcccgccctt cgaggacgcc gtgctcgaca tgtcgaaccc
      541 ggaggcggtg aagtggtatc aggcaaagct cgccggactg ctgaagatgg gggtcggcgc
      601 catcaaggtg gacttcggcg aaggcgcgcc gccgaacggg ctttacgctt cggggcgcac
      661 cggcctgcac gaacacaacc tctatcccct gcgctacaac aaggccgtcg ccgacatcac
      721 gcgcgaggtg acgggcgagg acatcatctg ggccagaagc gcgtgggccg ggagccagcg
      781 ttaccctctg cactggggcg gcgacgccga gaacacggac tcggcgatgg ccgccacgct
      841 gcgcggcggc ctctcgttcg gcctctcggg cttcacctac tggagccacg acgcgggcgg
      901 cttcgtgcgc cgcgcgccgc gcgacctcta ccggcgctgg atggcgtggg gcgtcctgac
      961 ctcgcacacg cgcgcgcacg gcgagccgcc gcgcgagccg tgggagtacg acgaggctct
     1021 gacggaagac ttccgccgcg cgctcggcct gaggtactcg ctcatgccct acatcctcgc
     1081 gcaggcgaag gactcctccg cgaaggggtt ccccctgctg cgcgcgctct tcttcgaata
     1141 ccccgaagac ccgacctctt ggacgattga cgaccagtac ctgttcggct ccgacctgct
     1201 cgtcgcgccg ctgttcgagg agggagcgac cgcgcgccgc gtctacctgc cgccgggcgc
     1261 gtgggtcgat taccagtcgg gcaagaccta ccgcggcgcc gcgtggcacc gcatcgaggc
     1321 ggggccgatt cccgtcatcc tgctggcgcg cgaccactcc gtcatcccgc acgtccgtgt
     1381 ggcgcagagc acggacggca tcgactgggg cgcggtcgag ctgcgcgtct tcagcacgga
     1441 cgacgcggcg gccacgggac tcttcaccac gccgcaaggc cccgtgcaga cgctccggct
     1501 cgtcccgcgc ggggggggtg cctacgccct cggcgaagac ccctcgggcg gtcgcgtcag
     1561 gtggcagatc aaccgcttcg cgacgcgttg agacgggcgg cgtgcggcgg gtcgtaaaac
     1621 gcggaacgcg tgaggtgaat gcccatgaac ttgaacaggt cactgacaac ggcgcgctgc
     1681 gtcgcggccc tgctcctggc gttcgtcgcc ctcggcccgc gccccgccac cacatcggcg
     1741 cagccgccac ggatggacac gatcctgtac ggtgcctcgt actaccacga gtacatgccc
     1801 gtcgagcggc tggacaaaga cgtgcagatg atgaaggacg ccggcatcag
//
LOCUS       DBNGOH010000829         2217 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1042173, whole genome shotgun sequence.
ACCESSION   DBNGOH010000829 DBNGOH010000000
VERSION     DBNGOH010000829.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2217)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2217)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2217
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1042173"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..492
                     /locus_tag="AC159P_16030"
     CDS             <1..492
                     /locus_tag="AC159P_16030"
                     /inference="COORDINATES: protein motif:HMM:NF012383.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HIR3697005.1"
                     /translation="FTAARACGMYEGALRAAVLTLKREPHVSARLARLLAETRRRAPL
                     SAATRIVPVPLHAEREGERGFNQAALLGRALAASEGLPLDAWSLVRVTHTARHRAGMD
                     ARARRETVEGAFRVARPRMVEGERVLLVDDVFTTGATVSACAAALRAAGAAEVFVLTV
                     ARA"
     gene            complement(511..807)
                     /locus_tag="AC159P_16035"
     CDS             complement(511..807)
                     /locus_tag="AC159P_16035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697006.1"
                     /translation="MNFVTLISQLLPLVGPLVPGGAQATLLAQAAANILAYIQQQKGL
                     TTEQILDRAGVQLDANEVELLADLARLQGTGGGGPSGPSGPSGGGSPSAPGGGQ"
     gene            complement(875..1351)
                     /locus_tag="AC159P_16040"
     CDS             complement(875..1351)
                     /locus_tag="AC159P_16040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697007.1"
                     /translation="MKHRRFFSASLVIALALTSLAFQCGGGGSQPEPLRNAARASDTI
                     AKSIGEMITVKRELAQQGKLTADEELKLTQALLRVNTADKVLVKRLKSLKAAPDATTK
                     AELVTMFGEVTSALNELNSGGLLGVRNEEARTRLTTIFNTINASVLIIQTFIDSSS"
     gene            1559..>2217
                     /gene="pip"
                     /locus_tag="AC159P_16045"
     CDS             1559..>2217
                     /gene="pip"
                     /locus_tag="AC159P_16045"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016873549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HIR3697008.1"
                     /translation="MRELYPPIEPYDQGRLPVSPVHTLYYEQCGNPEGLPVVFLHGGP
                     GGGCPPQYRQFFDPAAYRIVLFDQRGAGRSTPHAELEENTTWHLVADIERLREHLGVE
                     SWVVFGGSWGSTLSLAYAQTHPARARALILRGIFLCRPKEIRWFYQEGASWMFPDVWE
                     KYRDAIPEAERGDFVGAYYKRLTGDDERARLEAARAWSVWEGSTSKLFYDPALVEKFD
                     EP"
BASE COUNT          302 a          731 c          833 g          351 t
ORIGIN      
        1 ttcacggcgg cgcgcgcgtg cgggatgtac gagggcgcgc tgcgcgcggc ggtgctgacg
       61 ctgaagcgcg agccgcacgt ctcggcgcgg ctcgcgcggc tgctcgcgga gacccgtcgg
      121 cgcgcgcccc tctcggcggc gacgcgtatc gtgccggtgc cgctccacgc ggagcgggag
      181 ggcgagcgcg ggttcaatca ggcggcgctg ctgggccgcg cgctggccgc gtcggagggg
      241 ttaccgctcg acgcgtggag cctggtgcgc gtgacgcaca cggcgcgaca ccgcgcgggg
      301 atggacgcgc gcgcgcggcg cgagacggtc gagggcgcct tccgtgtcgc gcgcccgcgc
      361 atggtcgaag gcgagcgcgt gctgctcgtg gacgacgtgt tcacgacggg cgcgaccgtc
      421 tccgcgtgcg ccgcggccct gcgcgcggcg ggcgccgcgg aggtcttcgt cctcacggtc
      481 gcgcgtgcgt gaacaccgac ggaggggcgc ttactgcccg ccgcccggcg ccgacggcga
      541 gccgccgccg gaggggccgg aagggccgga agggccgccg ccgcccgtgc cttgcaggcg
      601 cgcgaggtct gccagcagct cgacctcgtt ggcgtcgagc tggacgccgg cgcggtcgag
      661 aatctgttcg gtcgtgagtc ccttctgctg ctggatgtag gcgaggatgt tggccgccgc
      721 ctgcgcgagg agcgtggcct gtgccccgcc ggggacgagc ggcccgacga ggggcaggag
      781 ctgcgagata agcgttacga agttcaagat ttcacctcca ggtgatgtgc tgcgctacgg
      841 tcggacgatt cccgaaaaac gcggtgccgg gccgtcagct agaggagtcg atgaaggtct
      901 ggatgatcag gacggaggcg ttgatggtgt tgaagatggt ggtcagccgg gtgcgcgcct
      961 cttcgttcct gacgccgagc agcccgcccg agttcagttc gttgagcgcg ctcgtgacct
     1021 cgccgaacat cgtcaccagc tcggccttcg tcgtcgcgtc gggcgcggcc ttcagcgact
     1081 tgagccgctt gacgagcacc ttgtccgccg tgttcacgcg gagcagcgcc tgcgtcagct
     1141 tcagctcttc gtcggcggtg agcttgcctt gttgcgccag ttcgcgcttg accgtgatca
     1201 tctcgccgat ggacttggcg atggtgtcgg aagcgcgggc ggcgttgcgc agcggctccg
     1261 gctgtgaacc gccgccgccg cactggaacg cgagcgaggt cagcgccagt gcgatgacga
     1321 gggacgcgga aaagaatcgt ctgtgtttca tgtgggcctc ctttgtggcc gttgacgagg
     1381 tgcgcagtgg acggacagtg aggacgccca acgccttcac gtctgggcgt cggattagag
     1441 ggaagcaaga gcctttcgcg gcggcgggta ttctacgcca aacgctccgc gtgtcaagga
     1501 ctttggtcgc gcgcgcgccg ctggcccgcc cgtcgcgcgc cgctgtatga tgtcgcgcat
     1561 gagagaactc tacccgccga tcgagccgta cgatcaggga cggctgccgg tctcgccggt
     1621 gcacacgctg tactacgagc agtgcggcaa ccccgagggg ctgccggtcg tcttcctgca
     1681 cggcggcccc ggcggcggct gcccgcccca gtaccgccag ttcttcgacc ccgcggccta
     1741 ccgcatcgtc ctcttcgacc agcgcggcgc gggccggagc acgccgcacg ccgagctgga
     1801 agagaacacg acgtggcacc tcgtcgcgga catcgagcgg ttgcgcgagc acctcggcgt
     1861 cgagagctgg gtcgtcttcg gcggctcgtg gggcagcacc ctctcgctcg cctacgcgca
     1921 gacgcaccct gcgcgcgcgc gcgcactcat cctgcgcggc atcttcctct gccgcccgaa
     1981 ggagattcgc tggttctatc aggagggcgc gagctggatg ttcccggacg tgtgggagaa
     2041 gtaccgcgac gccatcccgg aggcggagcg cggcgacttc gtcggcgcct actacaagcg
     2101 gctgacgggc gacgacgagc gggcgcggct ggaagcggcg cgcgcgtgga gcgtctggga
     2161 gggcagcacc tcgaagctct tctacgaccc ggcgctggtc gagaagttcg acgagcc
//
LOCUS       DBNGOH010000830         3341 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3240609, whole genome shotgun sequence.
ACCESSION   DBNGOH010000830 DBNGOH010000000
VERSION     DBNGOH010000830.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3341)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3341)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3341
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3240609"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(55..1131)
                     /gene="prfA"
                     /locus_tag="AC159P_16050"
     CDS             complement(55..1131)
                     /gene="prfA"
                     /locus_tag="AC159P_16050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014787592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HIR3697009.1"
                     /translation="MLEKLDQIEARYEALTEELSSPEVFSDQANYAKVSKQHRALGEM
                     VSKYREWKTIREELAGAKELFDSADDEEMRELARAEMDSLTARLEKNEEDLRVLLLPR
                     DPNDEKNVILEIRAGTGGDEATLFAAEMLRMYGRYAERQGWRMEILDASESGIGGVKE
                     AIAMIEGDKVYSKMKHESGVHRVQRVPQTETSGRIHTSAVTVAVLPEAEEVDVKIDAK
                     DLRIDTFCSSGPGGQSVNTTYSAVRITHLPTNLVVSMQDEKSQIKNREKAMRVLRARL
                     QELEEQKQHEALASERRAMVGSGDRSEKIRTYNFKENRVSDHRIGLTIHQLDLVMEGA
                     LDEIVNALVTHYQAEKLKAETVAA"
     gene            complement(1255..2457)
                     /locus_tag="AC159P_16055"
     CDS             complement(1255..2457)
                     /locus_tag="AC159P_16055"
                     /inference="COORDINATES: protein motif:HMM:NF018798.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HIR3697010.1"
                     /translation="MSANERDLIVGGQAVIEGVMMRTPNAYAVAVRRGDGTIKFTGAP
                     LPKWSDRVPLLKLPVLRGGAVLVQSMALGIKALNYSAGEAYADAEKEETVEVALTPAV
                     IAGEGDFAGITGATPGLIPVPTQPRPEEEMKRGTTASAVGSIAFALLFNVFLFIIAPL
                     VLTNAIFIGAGWAVPADAPAQTQAAQATTNADGAAAANATGAATAGQPWYARAWGAVR
                     GYLRPVRPSVAFNLVDGAIRMIFFLVMIFSFSLMKDIRRVFEYHGAEHKTVFTWEAGL
                     PLTVENARTQPRQHPRCGTSFLMVVMLVSVVLFSVVKFDSLLYNMLVRVALVPVVAGI
                     SYEIIRLSARKEGGFIFKLITLPGVWLQNITTQEPDDSQLEVAIVALKESLKLEPQPE
                     EVTVTPLA"
     gene            complement(2754..3002)
                     /gene="rpmE"
                     /locus_tag="AC159P_16060"
     CDS             complement(2754..3002)
                     /gene="rpmE"
                     /locus_tag="AC159P_16060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HIR3697011.1"
                     /translation="MKQGIHPEYKEIAVHCACGNQFTTRSTRKEELHLEICSACHPFF
                     TGKQKLIDTAGRVDRFNKRYGRKTEAAPAPAAEGAPSA"
BASE COUNT          527 a         1104 c         1066 g          644 t
ORIGIN      
        1 accgcggcgg agaagacgac tgtgaggact ttggctctca tcatccctct tcggctaagc
       61 cgcgaccgtc tcggccttca gcttctcggc ctggtagtgc gtgacgaggg cgttgacgat
      121 ctcgtcgagg gcgccttcca tgacgaggtc gagctggtgg atggtgaggc cgatgcggtg
      181 atccgagacg cggttctctt tgaagttgta ggtgcgaatc ttttcggagc ggtcgccgct
      241 gccgaccatc gcgcggcgct cgctcgccag agcctcgtgc tgcttctgct cttcgagctc
      301 ttgcagccgc gcgcgcagca cgcgcatcgc cttctcgcgg ttcttgatct gcgacttctc
      361 gtcctgcatc gagacgacga ggttcgtcgg caggtgcgtg atgcgcaccg ccgagtaggt
      421 cgtgttgacg ctttggccgc ccggtcccga agagcagaag gtgtcgatgc gcaagtcctt
      481 cgcgtcgatc ttcacatcca cctcttcggc ctcgggcagc acggcgacgg tgacggcgga
      541 ggtgtggatg cgcccgctcg tctcggtctg cggcacgcgc tggacgcggt ggacgcccga
      601 ctcgtgcttc atcttcgagt agaccttgtc gccctcgatc atggcgatgg cctccttgac
      661 gccgccgatg cccgactccg acgcgtcgag gatttccatc cgccagccct gccgctcggc
      721 gtagcgcccg tacatgcgca gcatctcggc ggcgaagagc gtcgcctcgt cgccgcccgt
      781 cccggcgcgg atttcgagga tcacgttctt ctcgtcgttc gggtcgcgcg gcagcaggag
      841 cacgcgcaag tcctcttcgt tcttttcgag ccgcgccgtg agcgagtcca tctccgcgcg
      901 cgcaagctcg cgcatctcct cgtcgtccgc cgagtcgaac agctctttcg ccccggcgag
      961 ttcctccctg atggtcttcc actcgcggta cttcgagacc atctcgccca gagcgcggtg
     1021 ctgcttgctc accttcgcgt agttcgcctg atcagagaag acctcgggcg aggacagctc
     1081 ctcggtcagt gcttcgtagc gcgcttcgat ttgatccagt ttctctaaca tggcttttag
     1141 cgattggcga ttggcggtca gctttttcgt ttgcgaagca gaccgctttc aaacaatgtc
     1201 cgagccgtcg ctagcgacaa aagctgacag ccaatcgcta acggctaatc gctcttacgc
     1261 gagcggcgtc acggtgactt cctccggctg cggctcaagc ttgagcgatt ccttgagtgc
     1321 gacgatggcg acttcgagct gcgagtcgtc cggctcctgc gtcgtgatgt tttgcagcca
     1381 gacgccgggc agcgtgatga gcttgaagat gaagccgcct tccttgcgcg ccgagaggcg
     1441 gatgatctcg tacgagatgc cggcgacgac cggcacgagc gcgacgcgca ccagcatgtt
     1501 gtagaggagc gagtcgaact tgacgaccga gaagagcacg accgagacga gcatcacgac
     1561 catcaggaag ctcgtcccgc agcgcgggtg ctggcgcggc tgcgtgcgcg cgttctcgac
     1621 cgtcagcggc agaccggcct cccacgtgaa gacggtcttg tgctccgcgc cgtggtactc
     1681 gaagacgcgg cggatgtcct tcatcagaga gaagctgaag atcatgacga ggaagaagat
     1741 catccgtatc gcgccgtcca ccagattgaa agcgaccgaa gggcgcacgg gccgcaggta
     1801 gccgcgcacc gcgccccacg cgcgcgcgta ccacggctgg cccgccgtcg ccgcacccgt
     1861 cgcgttcgcc gccgcggcgc cgtccgcgtt cgtcgtcgcc tgcgcggctt gcgtctgcgc
     1921 gggggcgtcc gcggggacgg cccagcccgc gccgatgaag atggcgttgg tcaggacgag
     1981 cggcgcgatg atgaagagga agacgttgaa gagcagcgcg aaggcgatgg agccgaccgc
     2041 cgaggcggtc gtgccgcgct tcatctcctc ttcggggcgc ggctgcgtcg ggacggggat
     2101 gagtccgggc gtcgcgccgg tgatccctgc gaagtcgccc tcgcccgcga tgacggcggg
     2161 cgtcagcgcg acctcgaccg tctcctcctt ctcggcgtcg gcgtacgcct cgcccgccga
     2221 gtagttcagc gccttgatgc cgagcgccat cgactgcacg agcaccgccc cgccgcgcaa
     2281 gaccggcagc ttcaacagcg ggacgcggtc gctccacttc ggcagcggcg cgcccgtgaa
     2341 cttgatcgtg ccgtcgccgc ggcgcaccgc caccgcgtac gcgttcggcg tccgcatcat
     2401 cacgccctcg atcacggcct gcccgcccac aatcaaatct ctctcgttgg cgctcacgtc
     2461 gattcctttc gcaaaagacg taaatcgtga accgtcaacc gtgacgagta agtgcaggac
     2521 ttcccggcct ctcgtcaccg gtcacgggtt ttaaactcat cacggtttag ggttgacggt
     2581 tcacggcggt aaaaacgccg acgttcgggt tcagcggtct ctcgcggctg cgcttgttgc
     2641 ggggcgcacg ctcggcgacc gccggacgcg aaggcgtcgg cgtcaggttt tcacggctag
     2701 tagtggcggc gtcggttctc gttcatacgc gctgccgggc tgcggggctt acttcaggcg
     2761 ctcggcgccc cctctgcggc gggcgccggg gcggcctcgg tcttcctgcc gtagcgcttg
     2821 ttgaagcggt caacgcggcc cgccgtgtcg atcagcttct gcttgccggt gaagaacgga
     2881 tggcacgccg agcagatttc cagatgcagc tcttccttgc gcgtcgagcg cgtcgtgaac
     2941 tggttgccgc aggcgcagtg gacggcgatc tctttgtact cgggatgaat tccttgcttc
     3001 attttgattc ctcttccgta gggcggcggc ggcgccgcga atcgaacact gaatgatact
     3061 ttcgaggccc ggatatcgtc aagtcggaca accacttgga cgcgcgcggg cgcctccgag
     3121 ttcgccgccc cgacgcgcgc caacaacttc attgacagtc acgcaaaccg cgtgttacaa
     3181 cgctctactg tcacgccttt tcagcatcaa ggttacgcgc acaagtcaac tgcatttgtg
     3241 aagcaggacg agcggcagcc ccgcgctacc gccgcgcctc cgggtcgggc cgcaggatgc
     3301 gatgacagac gaaccgaaca acacctcccc gcctgacgaa g
//
LOCUS       DBNGOH010000831         4347 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4889864, whole genome shotgun sequence.
ACCESSION   DBNGOH010000831 DBNGOH010000000
VERSION     DBNGOH010000831.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4347)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4347)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4347
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4889864"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2774)
                     /locus_tag="AC159P_16065"
     CDS             complement(<1..2774)
                     /locus_tag="AC159P_16065"
                     /inference="COORDINATES: protein motif:HMM:NF047160.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HIR3697012.1"
                     /translation="MSPKTKRTSTPRTLDLRPTAFPARRLIAQVLAFLLLAQTIAPPA
                     LAAPAQGFSLASQGFSLASLGSRVFSSFAAVLDRVGVGARASSAPTVAVRRAAEDFGL
                     VLTPLSTAFAGHVGIEHHQPLRKLVVSANSPAGDPFNFESLDADGTHRPFSNLAGLTG
                     ALRIATARDAGLGTSLGGFQPGELFTGTGAAGVVARVAPDGATVQNPWVTLAGETGQL
                     GGLYVDRTGVFGGDLLAVTTSGGVWRVNSAGVAARVADLATPLAGVTTVIDDATKYGP
                     WAGKILVGAKEQELVYAVDAQGNSTSYQLGLAPQDIRVIPAHENFYGVDPAGGKIWGA
                     PDDAFAGIIGDILVAQGSPGVLARVLWNGTAFEVSEIAQVSKWEQITFSPAALAEVGG
                     VKDVYDEIAVVRHAPQLDSGRVEGALWQLLPETVVLDGTDVITSDLLVPGTPTVTVGG
                     GNPSFDGVIEGVENTQPTGYSVSISNNAVLRHVITRTDPIRLMPVLYPHVPAGTRDVS
                     LSNSNQTIGDPATLRHLTLSGQAGAVAVPPGTYGRFSAGGHTAFVLGVEGTTVPSVYN
                     LEELSLSGGSELRVVGPVRLTVKGNVSLVGSTVGAADDPGRLLLQIAQGALGVTGNGV
                     LYGIVRIPQGTVSIGGNGRVRGTVTCDRLDIYGNGVLQITESDVAPPPVNRPPAADAG
                     PDQTITLPTDVVSLNGVATDDGLPHGSTLTTLWTKVSGPGPVTFANAASAVTTATFTE
                     PGTYVLRLTASDSLLMDSDEMSVEVVPRNQPPIVNAGPDQSIELPNTATLAGVVSDDA
                     LPRGSTVTKTWSVLSGPGAVTFADPHDLSTVVTFGAPGDYTLRLTANDTEFTVSDDVL
                     VTVYPVNQPPVVNAGPDQTLRLPAAATLDGTATDDGWPFGSTLTVAWTKVSGPGTVTF
                     AGPTNTVRAA"
     gene            complement(2958..3437)
                     /locus_tag="AC159P_16070"
     CDS             complement(2958..3437)
                     /locus_tag="AC159P_16070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697013.1"
                     /translation="MRRSLPALFTTSLAVLVSVALAHAAATTYNGTLTGSQTASAESG
                     TVALNLNASGDLPGILNISLRHEGGNVNGGAFTLTVLPQNADASSSARGRLTGTVTGG
                     TLTLDANGIVSAAGAVTLAVQSGTDEYAGVTGGSGTLSLSADPENSTKLGGPFALDF"
     gene            complement(3512..>4347)
                     /locus_tag="AC159P_16075"
     CDS             complement(3512..>4347)
                     /locus_tag="AC159P_16075"
                     /inference="COORDINATES: protein motif:HMM:NF012801.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor domain-containing
                     protein"
                     /protein_id="HIR3697014.1"
                     /translation="ETLDNYEAGFKLHAGRVGATGSVFDAEFTNFIERRVLLLAPGAV
                     GSTVGGQPIIRQDASGAVFTSLSSAPVFVRANAGHIRLRGFEAAGLFDLTRSLSVGAH
                     AFYVRGTNTETGLPPSLENGIPPLTGFASVRWTPAGRSFWVETYTSFAAAQERFSDND
                     LQQARIGGIRTREEITQFFNHGAVARDLVRGGILLETGETLPQVLSRVLGPNLSARVP
                     LYTKNPGFATLNVRGGVRLGERMRVTLILENILDKNYRTMGSGVDAPGVNAMVSFSRS
                     F"
BASE COUNT          617 a         1407 c         1681 g          642 t
ORIGIN      
        1 taggcagctc tgacggtatt cgtggggccg gcgaaggtga cggtgccggg gccgctcact
       61 ttcgtccacg cgacggtgag cgtgctgccg aagggccagc cgtcgtcggt cgcggtgccg
      121 tcgagcgtcg ccgccgccgg caggcgcaac gtctggtcgg ggccggcgtt gacgaccggc
      181 ggctgattca cgggatagac cgtgacgagc acgtcgtcgc tgacggtgaa ctccgtgtcg
      241 tttgccgtga ggcgcaaagt gtagtcgccg ggcgcgccga aggtgacgac ggtcgagagg
      301 tcgtgcgggt ctgcgaaggt gacggcgccg gggccgctca agacgctcca cgtcttcgtc
      361 acggtcgagc cgcggggcag cgcgtcgtcg gacacgacgc ccgcgagggt cgccgtgttc
      421 ggcagctcga ttgattggtc aggcccggcg ttgacgatgg gcggctggtt gcgcgggacg
      481 acttcgacgc tcatctcgtc ggagtccatg agcaaagagt ccgaggccgt caggcgcagg
      541 acgtaggtgc cgggttcggt gaaggtggct gtggtgacgg cgctcgcggc attagcgaac
      601 gtgacggggc cggggccgga gaccttcgtc cacagggtcg tgagcgtgct gccgtggggc
      661 aggccgtcgt cggtcgcgac gccgttgagg ctcaccacgt cggtcgggag cgtgatggtc
      721 tggtcggggc cggcgtccgc tgcgggcggg cggttgacgg gcggcggcgc cacgtcgctc
      781 tcggtgatct ggaggacgcc gttgccgtag atgtcgagcc ggtcgcaagt gaccgtgccg
      841 cggacgcgac cgttgccgcc gatggagacc gtgccctgcg ggatgcggac gatgccgtag
      901 agcacgccgt tgccggtgac gccgagcgcg ccctgcgcga tttgcagcag cagccgccca
      961 gggtcgtccg ccgcgccgac cgtcgagccg acgagcgaca cgttaccctt caccgtcagc
     1021 ctgacggggc cgaccacgcg cagctcgctg ccgcccgaga gcgacagctc ttcgaggttg
     1081 tagacggagg gcacggtcgt gccctcgacg ccgaggacga aggccgtgtg gccgcccgcg
     1141 gagaacctgc cgtaggtgcc cggcgggacg gcgaccgcgc cggcctgtcc cgagagcgtc
     1201 aggtggcgca gcgtcgccgg gtcgccgatg gtctggttcg agttcgagag cgacacgtcg
     1261 cgcgtgccgg cggggacgtg cgggtagagc acgggcatga gccggatggg gtcggtgcgc
     1321 gtgatgacgt ggcgcaggac ggcgttgttg ctgatggaga cggagtagcc cgtgggctgc
     1381 gtgttctcga cgccctcgat gacgccgtcg aagctcgggt tgccgccgcc gaccgtgacg
     1441 gtcggcgtgc ccggcacgag caggtcggag gtgatgacat ccgtgccgtc gagcacgacc
     1501 gtttcgggca gcagttgcca gagcgcgccc tcgacgcgcc cgctgtcgag ttgcggcgcg
     1561 tggcgcacga cggcgatctc gtcgtagaca tccttgacgc cgccgacctc ggccagcgcc
     1621 gcgggcgaga aggtgatctg ctcccacttc gagacctgcg cgatctcgga gacctcgaac
     1681 gcggtgccgt tccagagcac gcgcgccagc acgccgggcg agccttgcgc gacgaggatg
     1741 tcgccgatga tgcccgcgaa ggcgtcgtcg ggcgcgcccc agattttgcc gcccgccggg
     1801 tcaacgccgt agaagttttc gtgcgcgggg atgacgcgga tgtcctgcgg cgcgaggccg
     1861 agctggtagc tggtggagtt gccctgcgcg tcaaccgcgt agacgagttc ttgctctttc
     1921 gcgccgacca gaatcttgcc cgcccacggg ccgtacttcg tcgcgtcgtc gatgacggtc
     1981 gtgacgcccg cgaggggcgt cgcgaggtct gcgacgcgcg cggcgacgcc cgcggagttg
     2041 acgcgccaca cgccgcccga agtggtgacg gccagcaggt cgccgccgaa gacgcccgtc
     2101 cggtcaacgt acaacccgcc gagctgcccg gtctcgcccg cgagcgtgac ccaggggttc
     2161 tgcaccgtgg ccccgtcggg ggcgacacgc gccacgacgc ccgccgcgcc cgtccccgtg
     2221 aacaactcgc ccggctggaa gcccccgagg ctcgtgccca acccggcgtc gcgcgccgtc
     2281 gctatccgca gcgcgccggt cagcccggcg aggttcgaga aggggcggtg cgtgccgtcc
     2341 gcgtccagcg actcgaagtt gaaggggtcg cccgccgggc tgttcgcgga gacgacgagc
     2401 ttgcgcagcg gctggtggtg ctcgataccg acgtgtcccg cgaaggccgt ggagagcggc
     2461 gtcagcacca gcccgaagtc ctccgccgca cgcctgacgg caacggtcgg agcggaggaa
     2521 gcgcgcgcgc cgacgcccac gcggtcgagc acggcggcga acgacgagaa gacgcggctg
     2581 ccgagcgagg cgagcgagaa gccttgcgaa gcgagcgaga agccttgcgc cggggccgcg
     2641 agggcgggcg gcgctatcgt ctgcgcgagc agcaggaacg ccaacacctg tgcgataagg
     2701 cggcgcgcgg ggaacgctgt ggggcggagg tcgagggtgc ggggcgtgct cgttcttttc
     2761 gtcttggggg acatcgtcag gctccttgaa ggtcgtgttg accgcgccgg ggtgggcgcg
     2821 cgtccgctcg cgaactgctc ggcgtatggt ggcgtgttgc ggcgaaggct cgactgatga
     2881 ggcgaggggt ctgcggtgcg cgccttcgcg ggtgcggcgc cgccttacag cagacggcgc
     2941 gcgcggtgcg gcgaggctca gaagtcgagg gcgaaggggc cgccgagctt cgtggaattc
     3001 tcggggtcgg cggagaggct gagggtgccg ctgccgcccg tcactccggc gtactcgtcc
     3061 gtgccgctct gcacggcgag agtgacggcg cccgccgccg acacgatgcc gttcgcgtcg
     3121 agcgtcaggg tgccgcccgt caccgtgccg gtcagccggc cccgcgcgct ggaggaggcg
     3181 tcggcgttct gcggcagcac ggtcagcgtg aaggcgccgc cgttgacgtt gccgccttcg
     3241 tgcctgagcg agatattgag gatgccgggc aaatcgccgc tcgcgttcag gttgagcgcg
     3301 acggtgccgc tctcggcgga agcggtctgc gagccggtca gcgtgccgtt gtaggtcgtg
     3361 gccgcggcgt gggccagcgc aaccgagacg agcaccgcga gcgatgtcgt gaagagggcg
     3421 ggaagcgaac gcctcatgat gtcgattctc ctcaaacggg ggttttcact ccgcgctccg
     3481 gcgggcagag gggccgccgc agcgctgcgc gtcagaaact tcttgagaaa ctgaccatcg
     3541 cgttgacgcc cggcgcgtca acgcccgaac ccatcgtgcg gtagttcttg tcgaggatgt
     3601 tttcgaggat cagcgtcacg cgcatccgct cgccgagccg caccccgccg cgcacgttga
     3661 gcgtggcgaa gccggggttc ttcgtgtaga gcggcacgcg cgccgagaga ttggggccga
     3721 gcacgcgact gagcacctgc ggcaaggtct cgcccgtctc aagcagaatg ccgccgcgca
     3781 cgaggtcgcg cgccaccgcg ccgtggttga agaactgcgt gatctcttcg cgcgtgcgga
     3841 tgccgccgat gcgcgcctgc tggaggtcgt tgtcggagaa gcgctcttgt gcggcggcga
     3901 acgacgtgta ggtctcgacc cagaaggagc ggcccgccgg agtccagcgc acggaggcga
     3961 agccggtcag cggcgggatg ccgttctcca gagacggcgg caggcccgtc tcggtgttcg
     4021 tcccgcgcac gtagaaggcg tgcgcgccca cggagagcga gcgcgtgagg tcgaagaggc
     4081 ccgcggcctc gaagccgcgc aggcggatgt gcccggcgtt ggcgcgcacg aagaccggcg
     4141 cgctggagag cgacgtgaag accgcgcccg aggcgtcctg ccggatgatc ggctggccgc
     4201 cgaccgtgct cccgaccgcg ccgggcgcga gcagcagcac gcgccgctcg atgaagttcg
     4261 tgaactccgc gtcgaagacc gagccggtcg cgccgacgcg cccggcgtgc agcttgaagc
     4321 cggcctcgta gttgtcgagc gtctcgg
//
LOCUS       DBNGOH010000832         9513 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1591196, whole genome shotgun sequence.
ACCESSION   DBNGOH010000832 DBNGOH010000000
VERSION     DBNGOH010000832.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 9513)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9513)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9513
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1591196"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            57..476
                     /locus_tag="AC159P_16080"
                     /pseudo
     CDS             57..476
                     /locus_tag="AC159P_16080"
                     /inference="COORDINATES: protein motif:HMM:NF012722.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
     gene            569..802
                     /locus_tag="AC159P_16085"
     CDS             569..802
                     /locus_tag="AC159P_16085"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR3697015.1"
                     /translation="MPGIGLGLHLARVLVEGMGGSIEARSRPGLGSTFTLRVPVWSDE
                     AEGVDGEAVAGVARRPALDATGAAAAREGLKHG"
     gene            912..1442
                     /locus_tag="AC159P_16090"
     CDS             912..1442
                     /locus_tag="AC159P_16090"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR3697016.1"
                     /translation="MRINEAVPDLIVSDIRMPGMDGYKLARALRANPRTNLIPVIFLT
                     AKDERRDRIEGIRAGVDAYLVKPFDPEELLAVVANILGRVERTSAELARLVNTATGDE
                     PSPPDDLAPVEDFTEAEARVARLVARGLSNKEIAAELGVSVRTVEGHVSNILSKKGWA
                     NRVEIARHVLGRGENG"
     gene            1842..2240
                     /locus_tag="AC159P_16095"
                     /pseudo
     CDS             1842..2240
                     /locus_tag="AC159P_16095"
                     /inference="COORDINATES: protein motif:HMM:NF012791.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2563..2775
                     /locus_tag="AC159P_16100"
     CDS             2563..2775
                     /locus_tag="AC159P_16100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697017.1"
                     /translation="MWVTGAAAIVAALLYWEQTAALYVLSTLAMSALLLVVAFSDLEG
                     KERVIRKRETGDEDDRRDVNQEVRRA"
     gene            2815..3315
                     /locus_tag="AC159P_16105"
     CDS             2815..3315
                     /locus_tag="AC159P_16105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007725977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter regulatory subunit"
                     /protein_id="HIR3697018.1"
                     /translation="MPTISESNLPGIGRKFQVETLCGDRLVVVIHDDGKRELYHFTRR
                     NPDRAASVLTLTDGEARQIAGIVGGLTYVPKALPSTEVVLEDLVLEWYTLEAGAACAG
                     KTIRDLQARTRSGASIVSIIEPNRVTRTNPEADTILNEGATLILAGDRRAIERLKRLL
                     LSGGDE"
     gene            3319..4173
                     /locus_tag="AC159P_16110"
                     /pseudo
     CDS             3319..4173
                     /locus_tag="AC159P_16110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017426653.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
     gene            complement(4362..6020)
                     /locus_tag="AC159P_16115"
     CDS             complement(4362..6020)
                     /locus_tag="AC159P_16115"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002632707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding response regulator"
                     /protein_id="HIR3697019.1"
                     /translation="MKKTGPKDERLLDAALLRWMNDLAGHGVLVTDAELKIRAWNRWL
                     EEHSELSARDVLGRNLLEVYTELEKRRLDRHYRWVLEGQTSILSQRLHGHLLPLPTDT
                     SMSGFSEMQQTARISPLIAEDGQIIGTLTVIEDVTERVAREAELQSQVDARTRALASE
                     KASREEAEEANRLKDEFLATVSHELRTPMTAILGWSNMLLTGRLAGEMHDRALETIHR
                     NAQAQNQLISDLLDISRIISGKLRLDLRMVDLSSVIEGAVEATRPAAEAKGIRLTTAL
                     DRRAGPINGDADRLQQVVWNLLTNAIKFTPEGGQILVKLESVNSRVEITVRDSGIGIS
                     PEFLPHVFDRFRQADPGTNRVHGGMGLGLSIVRQLVELHGGTARAESEGAGKGTTFAV
                     SLPFVTLHEETRRARRPAAAVAHLELDCPPSLRGLRALVVDDEADTRDMLRAVLEQCQ
                     VEVITAGSASEALEALVRSRPDVLISDLGMPGEDGYDLISKVRALPAESGGRIPAAAL
                     TAYVRAEDRVRVLRAGFQLHVPKPIEPAELVIVVANLAGRMGTD"
     gene            complement(6055..6678)
                     /locus_tag="AC159P_16120"
     CDS             complement(6055..6678)
                     /locus_tag="AC159P_16120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015149419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheC"
                     /protein_id="HIR3697020.1"
                     /translation="MELTDRQRDAIAELINIAFARTGAALSELTGHRVMLNAPEVSVH
                     PTAELPAALAKFIPGEVASIHQVFGGPVAGDALLLLNHDGAVHLTDLLTDGSAPPSGR
                     LDESAREVLTEVGNILLNACLGMFGNLLDVRVSFSVPRLHLETLDELLDSLIGGDSEP
                     QYALVVYTAFHIRDSSVSGFLVMVLSVTSLDRLIQEVEAWEKRQENS"
     gene            complement(6683..7048)
                     /locus_tag="AC159P_16125"
     CDS             complement(6683..7048)
                     /locus_tag="AC159P_16125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR3697021.1"
                     /translation="MRKKIMVVDDSALSRRTLRRILEMAGYSVVEADEGMAALELYFI
                     EKPDLVLLDLVMKGMYGLEVLAKLREMDAGARVVVASADIQTSTRALVEEAGAHAFIT
                     KPFASENVLGAVESALRGE"
     gene            complement(7045..8088)
                     /locus_tag="AC159P_16130"
     CDS             complement(7045..8088)
                     /locus_tag="AC159P_16130"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008055453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate
                     methylesterase/protein-glutamine glutaminase"
                     /protein_id="HIR3697022.1"
                     /translation="MDRVLKVLVVDDSAYVRKVVKQMLSRSPFLEVVGTARDGREALE
                     LAERLDPDVITCDLIMPELDGVGFVREQMRRRPVPIIIMSIANETGEAALTALDAGAV
                     DFVQKPTALATEKIFEVSEELIEKVKAAARIPMARVRVDAAAPEAAPSPTSVAGSGLA
                     DIVVIGISTGGPQALKQLIPQLPADFPVPVVMVMHMPVGYTEMYARKLNEHAPLEVRE
                     AREGDVLRAGVALLAQAGRHLSFRRRPEGEVVAHLDSRPFEMQHRPSVDVLFQSAAEV
                     YGQRVLGVVMTGMGSDGKQGAAWIKAQGGLVYTESEETCVVYGMPSSVVEAGLSDRSV
                     PLDRMAQAIREAV"
     gene            complement(8117..8974)
                     /locus_tag="AC159P_16135"
     CDS             complement(8117..8974)
                     /locus_tag="AC159P_16135"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017747759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HIR3697023.1"
                     /translation="MGVNTGSLGITGGADRLLRDLIHERTGLFFDNGKGDILADKLSP
                     LVIERGFTSLLDYYYLLKYDDAAQDEWLNVMNALSVQETYFWRELDQVRALVEWVVPR
                     WFSAPGAGPLKIWSAACATGEEPLTIAMALDEAGWLGRVPVEIHASDASTSAIAKARR
                     GVYRERSFRNLPAELRAKYFTQEAATKTWRIDAKLHARIRWGVVNLMNEAEAAPWAAA
                     NVIFCRNVFIYFSDQTIGRTVRSFAAHIRPPGYLFVAASESLLRLSADFELQEIGSAF
                     VYVKRPGRG"
     gene            complement(9017..>9513)
                     /locus_tag="AC159P_16140"
     CDS             complement(9017..>9513)
                     /locus_tag="AC159P_16140"
                     /inference="COORDINATES: protein motif:HMM:NF025030.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HIR3697024.1"
                     /translation="ISHTDAQPPLFEALHSPDAEVRAEAAAALGERGGAEAVERLRVV
                     AATDAAPSVVEAATDALERVATPEAIAGLLALAADPSRYELAVAALARTREALLEEVA
                     RGLSPQRPAEVRRAVVEALGRMKSRRAAELLRGAYDDADAAVRSAARGYGRTAFGKPP
                     GDIG"
BASE COUNT         1470 a         3369 c         3121 g         1553 t
ORIGIN      
        1 tcaaggacga gttcctcgcc gccgtctcgc acgagctgcg gacgcccctg acgaccatca
       61 aggctttgac gcgcgtgctc ttgaggaaga accccacgga ggaagagcgg cgcgagtacc
      121 tcgcggacat cgcctccgag tgcgaacggc agatcgacct cgtgcataac ctgctcgacc
      181 tctcgcgcat caacgcgggc ggcgtgcaga tcgagctgcg gcgcgtcagc gccgaagatg
      241 tcgtgcgcgc gtgcgagaag atcgagcgcg tcgaggccgc cgaacacggg catgagctga
      301 gagtcgaagt cgctccagac cttccgccca tccgggccga ccacagcgcg ctgcgccgcg
      361 ccctctgcac cgtcgtcggg aacgccatca ggtacacgcc cgacggcgga agcatcgcgc
      421 tgcgcgcaga gagcgacggc ggcacggtcg ccttcgaggt cgaggacaac gggcgcggca
      481 tccaccccgc agacctgccg cacatcttcg agagcttcta ccgcgggcgc gccgcggcga
      541 cgagtcagcg ggcacggacg agcaggaagt gcccggcatc ggcctcggcc tccatctggc
      601 gcgcgtactc gtcgagggga tgggcggctc gatcgaagcg cggagccgtc cgggcttggg
      661 gagcaccttt acgttgcgcg tccctgtctg gagcgacgaa gcggagggcg ttgacggcga
      721 agcggtcgcg ggcgtcgcgc gccgccccgc actggatgcg acgggcgcgg cggcggcgcg
      781 agaggggctg aagcatggct aagcgtttgc tcgtcgtgga cgacgaggcg aagctcttgc
      841 gggccgttgc cgtcacgctg cgcggcgaag gctacgaggt gacgaccgca cggagcggcg
      901 cgtaggcgct ggtgcgcatc aacgaggcgg tgcccgacct gatcgtcagc gacatccgca
      961 tgcccggcat ggacgggtac aagctcgcgc gcgcgctccg cgcgaacccg cgcacgaacc
     1021 tcatccccgt catcttcctc accgcgaagg acgagcgcag agaccgcatc gagggcatcc
     1081 gcgccggcgt cgatgcctac ctcgtcaagc cgttcgaccc ggaagaactg ctcgccgtcg
     1141 tggcgaacat actgggccgc gtcgagcgca ccagcgccga actcgcgcgc ctcgtcaaca
     1201 ccgccacggg cgacgaaccg tcgccgcccg acgacctcgc gcccgtcgaa gacttcacgg
     1261 aggccgaagc gcgcgtggcg cgcctcgtcg cgcgagggct gagcaacaaa gagatcgccg
     1321 ccgaactcgg cgtcagcgtg cgcaccgtcg aaggccacgt cagcaacatt ctctcgaaga
     1381 agggctgggc caaccgcgtc gagatcgcac gccacgtcct cggacgcggg gagaacggct
     1441 agacgacttc cgccgctcct cggtgcgccg accgctgacg gttcctcaac cccgcttagc
     1501 ttcttctttc gcccgacgga aatccgccgt gcctcacagc cgcttgcaat cggacgtgac
     1561 ccgtgcgtgt tgactgtagg ggcagggctt gtccctgccc gtcgtcacat ccattcgacg
     1621 ttgaatccat tgagactcgc gggcagggac aagccctgcc cctacacctg tccccacggc
     1681 ctcgctcatt tgcaaaccgc tatcagacca cgtgaaaaca cctgcgcgga gcaagtgaag
     1741 tcacggtttc tcgcacggcc cgcattcttt aagctccacg cacgtcaacc ggcgagcggt
     1801 gtgaccggag agggcgtcgc gtggggcaaa aactgttgag ggtgttgatc gcttatgacg
     1861 ggtcggagtg cgccgccgcc gcgctcgacg acctgccgcg cgcgggcttg cccgacgtgg
     1921 gccggttcct gatcgccacg gtggccggga gcctcaagtt gagtcccttt ccggcgagcc
     1981 accagctcat agagaaggcc gtcaacgcac ggagggtggt gtcggccatc gcgttggtgc
     2041 gggaagagac cacgcgagag gtggcgaagg cgcaaaggct gtcgtccgag gccggttgcc
     2101 gcgtgcaggc ttgcttcccg cggtgggacg tgctggccga ggcgttgacg ggcgagccgt
     2161 ccgtcgcgtt gctcgggagg gccgagacgt gggcggccaa cctcgtcgtc gtcggcacac
     2221 gagggcgctc cgcaatcggg cggttcttcc acggcagcgt gtcgaaaaag gtcgccgaag
     2281 aggcgcgctg ctcggtgcgt gtggggcgtc gcggtttcga taagcgtgaa gacgaagccc
     2341 ccagaatcat cgtcggcgtg gacgggtcgg agggagcggg cgaggccgtc cgggcggtgt
     2401 gcgcgcgcac gtggcccgcg ggcacggagg tacgcgtggt cgccgtccat gacgcggccc
     2461 cgcccgccag acccgcggac gctcccacgg gcacggcggc gcacatcgcc ggcccggtcg
     2521 aaacggggcc gccgcccgtg cgcctggcgg cgcggcgcgt tcgtgtgggt cacgggcgcg
     2581 gcggccatcg tcgccgcgtt gctctactgg gagcagacgg ctgcgctcta cgtgctctcg
     2641 acgctggcga tgagtgcgct gctgctcgtc gtcgcgttct cggacttgga gggcaaagag
     2701 cgggtgattc gaaagcgcga gaccggcgac gaggatgatc gccgagatgt taaccaggaa
     2761 gtgcggcggg cttagccgaa gctgttcgcg caaggcccgc gagaggtatt catcgtgccg
     2821 accatcagcg aatcgaatct ccccggcatt ggccgcaagt ttcaggtcga gaccctgtgc
     2881 ggcgaccgcc tcgtcgtcgt catccacgac gacggcaagc gcgagctgta tcacttcacg
     2941 cgccggaacc ccgaccgcgc ggcctctgtg ttgacgctga ccgacggcga ggcgcgccag
     3001 atcgcgggca tcgtcggcgg gctgacctac gtccccaaag ccctgccctc cacggaagtc
     3061 gtcctcgaag acctcgtcct cgaatggtat acgcttgagg ccggcgcggc gtgcgcgggc
     3121 aagaccatcc gcgacctaca ggcgcgcacg cgctcgggcg cgagcatcgt ctcgatcatc
     3181 gagccgaacc gcgtgacgcg caccaacccg gaggccgaca cgatactcaa cgagggcgcg
     3241 accctcatcc tcgccggcga ccggcgggcg atagagcggc tcaagcgcct gctcttgagc
     3301 gggggagacg agtagtcgat ggatcacctc gtccttgagg tcggcctcgc gctcgggctg
     3361 gtggccgccg ccgcgttgct gtccgcccgc ctgcgcttct ccgtcgtccc gttcctcatc
     3421 ctgacgggga tggcggtcgg cccgcacgcg ccgaaggtcg gccccttcga cttccgcttc
     3481 atcgagagcg cgccgctcat cgagttcatg gggcgcgtcg gcgtcctctt cctgctcttc
     3541 tacctcgggc ttgagttctc cgtcagccga ctgatcaagg cgggccgctc catcgccgtg
     3601 ggcggctcga tctacatcgc catcaacttc gccgcgggcc tgctttacgc cttcctgttg
     3661 ggctggccct tgaaggaggt gctggtcgtc gcaggcatca cgaccatctc ttcgagcgcc
     3721 atcgtcgcca aggtgctctt cgatctacgg cggacggcca accccgagac ggagatgacc
     3781 ctcggcatca tcatgttcga ggacgtgttc ctcgccgtct acctctcgct cgtctccggc
     3841 gtcgtcctga gcggcgcgac ttcggtcggc ggcgtcctga cctcggcggg gctggcgctc
     3901 gccttcatcc tcgggccgat cgtcttcggg agggcggcga cgccgctcct caaccgcgtg
     3961 ttccgcatct cttcgaacga agtcttcctg ctcgtgctct tcgcctgcct gttcctgctg
     4021 gcgggcctcg gcgagaccat ccacgtcgcc gaggccatcg gcgcgctgct gctcgggctg
     4081 gtgctggccg agaccgagca cggcgagcgc atcgaacacc tcatcgtgcc gttccgcgac
     4141 ttcttcggcg cgctcttctt cttcggcttc gggccgctgc tgacgaagga gtccgagcgc
     4201 atccacgacc tgtcggcgaa gttgtggaat cgaagtcggg cggaggacga ggcttccgcc
     4261 gaggcagggg tggtcaacag aaattagcga gcattactcg tgcgggcggc gaggagggcg
     4321 tgtcaactcg tttctcgcgg gacagccacg ttcctgcccc ttcagtccgt ccccatacgc
     4381 ccggcgaggt tcgccaccac gatcaccagc tccgccggct cgataggctt cggcacgtgc
     4441 aactggaaac cggcgcggag caccctcacg cggtcttccg ccctgacgta cgcggtcagg
     4501 gccgccgccg gaatccgccc gccgctctcc gccggcagcg ccctcacttt ggagatgagg
     4561 tcgtagccgt cctcgccggg catcccgagg tcgctgatca aaacgtcggg gcgcgagcga
     4621 acgagagcct ccagcgcctc ggaggccgac cccgccgtga ttacctcgac ctggcactgt
     4681 tcgagcaccg cgcgcagcat gtcgcgcgtg tcggcctcgt catcgacgac gagcgcccgc
     4741 aggccgcgga gagagggcgg gcagtcaagc tccaggtggg ctaccgcggc ggctggacgc
     4801 cgcgcccttc gcgtctcttc gtggagggtg acgaagggca ggctgacggc gaaggtcgtc
     4861 cccttcccgg cgccctcact ctcggccctc gccgtgccgc cgtgcagctc gaccagttgg
     4921 cggacgatgg acagccccag ccccatgccc ccgtggactc tgttggtgcc ggggtcggcc
     4981 tggcggaagc ggtcgaagac gtgcggcaaa aactcggggc tgatgccgat cccggagtcc
     5041 cgcaccgtga tctccacacg cgagttgacg ctctccaatt tgacgagtat ctgcccgccc
     5101 tcgggcgtga acttgatggc gttcgtcaaa aggttccaga ccacctgctg caaccggtcg
     5161 gcgtccccgt tgatcggccc ggcgcgccgg tcgagcgccg tcgtgaggcg gatgcctttc
     5221 gcctcggctg ccgggcgggt cgcctcgacg gccccttcga tgaccgaaga gagatcgacc
     5281 atgcgcaggt cgagccgcag cttgccgctg atgatgcgcg agatgtcgag caggtcgctg
     5341 atcaattgat tctgcgcctg cgcgttgcgg tggatcgttt cgagggcgcg gtcgtgcatc
     5401 tctccggcga gcctgccggt gagcagcatg ttcgaccagc cgaggatggc cgtcatgggc
     5461 gtccgcagct cgtgcgagac cgtcgcgagg aactcgtcct tgaggcggtt cgcctcctcc
     5521 gcctcttcgc gcgaggcctt ctcgctggcg agcgcacgcg tgcgcgcgtc cacctgcgac
     5581 tgcaattccg cctcgcgcgc cacgcgctcg gtcacgtcct cgatgacggt caaagtcccg
     5641 atgatctggc cgtcctcggc gatgagcggc gagatgcgcg ccgtctgctg catctccgag
     5701 aagccgctca tgctcgtgtc cgtcggcagc ggcaacaggt gcccgtgcag gcgctgcgaa
     5761 agaatgctcg tctgaccttc cagcacccac cggtagtgcc gatccagccg cctcttctcc
     5821 agctcggtgt agacttccag aaggttgcgc ccgagcacgt cccgcgcgct cagctcgctg
     5881 tgctcttcca gccagcggtt ccaggcgcgt atcttcaact cggcgtcggt gacgaggacg
     5941 ccgtgccccg cgagatcgtt catccaacgc agcagcgccg cgtcgagcag cctctcgtcc
     6001 ttcgggccgg ttttcttcat ggctttacga ggtgtgccac ggtcgtggct gcgtttatga
     6061 gttctcctgc cgcttctccc aggcttcgac ctcctgaatc agccggtcga gggaggtgac
     6121 gctcaagacc atcacgagga acccgctgac ggagctgtcg cggatgtgaa aggccgtgta
     6181 gaccaccagc gcgtactgcg gctcgctgtc gcccccgatg agcgagtcga gcagctcgtc
     6241 gagcgtttcc aggtgcaggc gcgggaccga gaacgagacg cgcacgtcga gcaggttgcc
     6301 gaacatcccg aggcaggcgt tcagcaggat gttccccacc tcggtcagca cttcgcgggc
     6361 cgactcgtcg agccgccccg aagggggggc gctcccgtcc gtcagcagat ccgtcagatg
     6421 caccgcgccg tcgtggttga gcagcagcag cgcgtcgccc gcgaccggcc cgccgaagac
     6481 ctggtggatc gaggcgacct cgccggggat gaacttggcg agcgccgccg gcagctcggc
     6541 ggtcgggtgg acggagacct ccggcgcgtt gagcatgacg cggtgcccgg tcagctcgga
     6601 gagcgcggcc ccggtgcgcg cgaacgcgat gttgatcagc tccgcaatcg cgtcgcgctg
     6661 gcggtcggtc agttccatcg ctctactccc ccctcaacgc ggactcgacc gcgccgagca
     6721 cgttctccga cgcgaacggc ttggtgatga acgcgtgcgc gcccgcctct tcgaccagcg
     6781 cccgcgtcga ggtctggata tcggcgctgg ccacgacgac gcgcgccccc gcgtccatct
     6841 cgcgcagctt ggccagcacc tccagcccgt acatgccctt catcaccaga tcgaggagga
     6901 cgaggtcggg cttctcgatg aaatagagtt cgagcgcggc catgccctcg tcggcctcga
     6961 cgacgctgta gccggccatc tccagaatgc gccgcagggt tctgcgcgac agcgccgagt
     7021 cgtccacgac catgattttt tttctcatac cgcctcccga atcgcctgcg ccatcctgtc
     7081 gagcggcacg ctcctgtcgc tcaggccggc ctccaccacc gaggacggca tgccgtagac
     7141 cacgcacgtc tcttcgctct cggtatacac aaggccgccc tgtgccttga tccacgccgc
     7201 gccctgcttg ccgtccgacc ccatccccgt catcaccacg cccagcaccc gctgcccgta
     7261 aacttcagcc gccgactgga agagcacgtc caccgacggg cggtgctgca tctcgaacgg
     7321 cctcgaatcg aggtgcgcga ccacctcgcc ttcggggcgg cggcgaaagc tcaggtgccg
     7381 cccggcctgc gccagcagcg ccacaccggc gcgcagcacg tcgccctcgc gcgcctcgcg
     7441 gacttcgagc ggcgcgtgtt cgttcagctt gcgcgcgtac atttcagtgt agccgaccgg
     7501 catgtgcatg accatcacga cgggcacggg gaagtccgcg ggcagttgcg ggatcaactg
     7561 tttcagcgcc tgcgggccgc cggtggaaat cccgatgacc actatgtcgg ccagcccact
     7621 gcccgcgacg gaagtcggtg acggcgccgc ctccggggcc gccgcatcga cacggacgcg
     7681 cgccatcggg atgcgcgcgg cggctttgac cttctcgatc agctcctcgc tgacctcgaa
     7741 gattttctcg gtcgccagcg ccgtcggctt ctgcacgaag tccaccgcgc ccgcgtcgag
     7801 cgcggtcagc gccgcctcgc cggtctcgtt cgcgatgctc atgatgatga tcggcacggg
     7861 ccgccgccgc atctgttcgc gcacgaaccc gaccccgtcc agctcgggca tgatgaggtc
     7921 gcaggtgatg acatcggggt cgagccgctc cgccagctcc agcgcctcgc gcccgtcgcg
     7981 cgccgtcccc acgacttcga ggaacgggct gcgcgagagc atctgcttga cgaccttccg
     8041 cacgtaggcg gagtcgtcca ccaccagaac tttgagaact cgatccatta tcgtcctcgg
     8101 agcgcgcggc ggtcggtcaa ccccggccag gccgcttcac gtacacgaat gcgctcccga
     8161 tctcctgcaa ctcgaagtcc gccgaaagtc tcaacaggga ttccgaggcg gccacgaaca
     8221 gataccccgg cgggcggatg tgggccgcga aagagcgcac cgtcctgccg atggtctggt
     8281 cggagaagta gatgaacacg ttgcggcaaa agatgacgtt cgccgccgcc cacggcgcgg
     8341 cctccgcctc gttcatcaaa ttaacgacgc cccacctgat gcgggcgtgc agcttagcgt
     8401 caatgcgcca cgtttttgtc gcggcctcct gcgtaaagta tttggcgcgc aactccgccg
     8461 gcaggttgcg gaacgagcgc tcgcggtaga ccccgcgccg ggccttggcg atggccgacg
     8521 tgctggcgtc gctggcgtga atctcaacgg gcacgcgccc gagccagccc gcctcgtcga
     8581 gcgccatcgc gatggtcaaa ggctcttcgc ccgtcgcgca cgccgcgctc caaatcttca
     8641 gcggcccggc gccgggcgcc gagaaccaac gcggcacgac ccactcgacg agcgcgcgca
     8701 cctgatccag ctcgcgccag aagtaggtct cctgcaccga gagcgcgttc atcacgttca
     8761 gccactcgtc ctgcgccgcg tcgtcgtact tcagcaggta gtaataatcc agcagcgacg
     8821 tgaacccgcg ctcgatgacc agcggcgaca gcttatccgc cagaatgtcg cccttcccgt
     8881 tatcgaaaaa cagccccgtg cgctcgtgta tcaggtcgcg cagcagcctg tcggccccgc
     8941 cggtgatgcc taagctaccc gtgttgacgc ccattgttct tcagcccgcg accgacctgc
     9001 cggaacctct ccgccgtcag ccaatgtctc ccggtggttt gccgaaggcg gtgcgcccgt
     9061 aacctctcgc cgccgaacgc accgcggcgt ccgcgtcgtc gtacgccccg cgcaacagtt
     9121 ccgcggcgcg ccggctcttc atccgcccca gcgcctcgac gaccgcgcgc cgcacctctg
     9181 ccggtcgctg gggcgacaac ccgcgcgcga cctcttcgag cagcgcctcc ctcgtccgcg
     9241 cgagcgcggc gaccgccaac tcataacggg acgggtcagc cgcgagagcc aacagcccgg
     9301 caatcgcttc cggcgtcgcc acgcgttcga gcgcgtccgt cgccgcttcg acgaccgagg
     9361 gcgcggcgtc cgtcgcggcc acgacccgca ggcgttccac ggcctccgct cccccgcgct
     9421 cgccgagggc ggcggcggcc tccgccctga cttcggcgtc cggcgaatga agcgcctcga
     9481 agagcggcgg ctgcgcgtcc gtgtgcgaga ttc
//
LOCUS       DBNGOH010000833         3423 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2874132, whole genome shotgun sequence.
ACCESSION   DBNGOH010000833 DBNGOH010000000
VERSION     DBNGOH010000833.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3423)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3423)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3423
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2874132"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..392
                     /locus_tag="AC159P_16145"
     CDS             <1..392
                     /locus_tag="AC159P_16145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697025.1"
                     /translation="ERLERAGRLARPKHWLDFAPFVMAHAPLKMTIEEARVETRRAWE
                     ASYSPERNAEAVAAIADRSFQYRASHLVARLFFRGIYFPQMTRGAWLKLIADNRRVIY
                     QLAKEATGQWRKARRKRADVSVEARAS"
     gene            479..1966
                     /locus_tag="AC159P_16150"
     CDS             479..1966
                     /locus_tag="AC159P_16150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012882328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HIR3697026.1"
                     /translation="MKVLLVYPEFPDTYWSFRHALTFEGKRSAFPPLGLLTVSAMLPE
                     KWERRLVDMNVRHLENADIEWADIVFASAMIVQKESLENVVRMCKARGKRVVVGGPYV
                     STSAEQLPEADHVFVGEAEATLPEFLHDLERGEAREVYKAAERPALTSTPIPHFRLAE
                     LDRYSAMSVQYSRGCPFSCEFCDIIEIYGRVPRTKTNEQMLAELDALCDVGWRGLVFV
                     VDDNFIGNKRNVRALLPDLIEWSERHGRPFSFITEASVNLAEDDALLEMMRRANFRRV
                     FLGIETPVEESLKEARKGQNTRRDLLESVRKIQGYGMEVMAGFIVGFDNDPEDIFDRQ
                     IEFIRESAIPLAMVGLLTALPDTQLWRRLKREGRLLKESGGNNVAIEGSLNFIPRMDT
                     ARLVEGYRRILRTIYSPAEYYQRALDCLSRLTEEPEPRRGHLAGDAAAFVRVVLALGV
                     RDGARSEFWRYMRRAASSHRRNFAHAVTLAAMGYHFRKLTEAFCE"
     gene            complement(2107..2805)
                     /locus_tag="AC159P_16155"
     CDS             complement(2107..2805)
                     /locus_tag="AC159P_16155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017319285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HIR3697027.1"
                     /translation="MPNHSRPPPIRNRLLAALPREEYERLLPHLKSVPLPFMKILYEG
                     GEPIRHVYFPGDGLISLLVVMGDGTVREIGVIGNEGMLGTAVALGMNTAPHRALIQLP
                     GSALRLKAGALRDELVRGGALPHVLRRYAHALFTQVSQSAACVSSHAVDKRLSRWLLM
                     THDHAPADEFEMKHEFMAMMLGVTRSVVTRAAGHLQKEKMIRYTRGRVTVLDRKRLEA
                     TACECYGVVKAGSL"
     gene            3007..>3423
                     /locus_tag="AC159P_16160"
     CDS             3007..>3423
                     /locus_tag="AC159P_16160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HIR3697028.1"
                     /translation="MPQSTLEPAGNRLLAKLPPEEYQRLLPHLEHVSFALGEVIYESG
                     GRQSYIYFPTTAIISLLYLMENGSSAEMGVAGHEGLVGVALFMGGNTVPNRAVVQSAG
                     TAFRMKTKVLRDEFARGGMFQRLLLRYTQALMTQMSQ"
BASE COUNT          604 a         1086 c         1139 g          594 t
ORIGIN      
        1 acgaaagatt ggagagggcg gggcggctgg cgcgccccaa acactggctc gacttcgcgc
       61 ccttcgtgat ggcgcacgcg cctttgaaga tgacgatcga ggaggcgcgg gtggagacca
      121 gacgtgcgtg ggaggccagc tacagcccgg agcgtaacgc cgaggccgtc gcggcaatcg
      181 ccgacagatc gttccagtat cgcgccagcc acctcgtcgc gcgcctcttc ttccgcggca
      241 tctacttccc gcagatgacc cgcggcgcgt ggttgaaact gatcgcggac aaccggcggg
      301 tcatttacca gctcgcgaag gaagcgaccg gccagtggcg caaggcgcgg agaaagagag
      361 ccgacgtttc ggtggaggcc cgcgcgtcgt gaagcaacgc atctgcccgg cgccgctcac
      421 cgagtcggca tgctgaccct ccgcggtcgg cgcaagactc gagcgaggag gtcgcacgat
      481 gaaggtatta ctcgtctacc cggagttccc ggacacctac tggagcttcc ggcacgcgct
      541 cacgttcgag ggcaagcgct cggccttccc gccgctcggg ctgctgaccg tctccgcgat
      601 gctgcctgag aagtgggagc ggcggctcgt ggacatgaac gtgcggcatc tggaaaacgc
      661 cgacatcgag tgggccgata ttgttttcgc cagcgcgatg atcgtgcaga aggagtcgct
      721 cgaaaacgtc gtccggatgt gcaaggcgcg cggcaagcgg gtcgtcgtcg gcgggccgta
      781 cgtctccacc agcgccgagc aattgcccga ggccgaccat gtcttcgtcg gcgaagccga
      841 ggcgacgctg ccggagttcc tgcacgacct ggagcgaggc gaggcgcgag aagtctacaa
      901 ggccgccgag cgcccggcgc tcacctcgac gcccatccca catttccgct tggccgaact
      961 cgaccgctac agcgcgatga gcgtgcagta ctcgcgcggc tgccccttca gttgcgagtt
     1021 ctgcgacatc atcgagattt acgggcgcgt cccgcgcacc aagaccaacg agcagatgct
     1081 ggccgaactc gatgccctgt gcgatgtcgg gtggcgcggc ctcgtcttcg tcgttgacga
     1141 caacttcatc gggaacaagc gcaacgtacg tgcgttgctg cccgacttga tcgaatggtc
     1201 ggagcggcac gggcggccct tctccttcat cacggaggcg agcgtgaacc tcgccgaaga
     1261 cgacgcgctg cttgagatga tgcggcgcgc gaacttccgc cgcgtcttcc tcggcatcga
     1321 gacgccggtc gaggagagcc tgaaggaagc gcggaagggg cagaacacgc ggcgcgactt
     1381 gttggagtcg gtgcggaaga ttcaagggta cgggatggag gtcatggcgg gcttcatcgt
     1441 ggggttcgac aacgaccccg aagacatctt cgaccgccag atcgagttca tccgcgagag
     1501 cgccatcccg ctggcgatgg tcgggctgct gacggcgctg ccggacactc agctctggcg
     1561 gcgtttgaag cgcgagggga ggctgctgaa ggagagcggg gggaacaacg tcgccatcga
     1621 ggggtcgctc aacttcatcc cgcggatgga cacggcccgc ctcgtcgaag ggtacaggag
     1681 gattctgcgc accatctaca gccccgcgga atactaccag cgggcgctcg attgcctgtc
     1741 gcgtttgacc gaggagccgg agccgcgccg cggacatctc gccggcgacg cggcggcgtt
     1801 cgtgcgcgtc gtcctcgcgc tcggggtgcg cgacggcgcg cggtcggaat tctggcgcta
     1861 catgaggcgc gccgcgagtt cacaccgacg caacttcgcg cacgccgtca cgctggccgc
     1921 gatgggctac cacttccgca agctgaccga agcgttctgc gagtaggcac cgccgccgct
     1981 gccgccgtgg cctgcgtgtc cgtgtgtttg gatagcttcg attaatccgg cgccttagcg
     2041 cgtccgtcgc ccatcgtcgg attacgactt tcccgaacct gtcgaaagta tgcgacccgc
     2101 ggctggttac aggctccctg ctttgactac cccgtagcac tcgcaggcgg tcgcctccag
     2161 cctcttccgg tcgaggacgg tcacccgccc acgggtgtag cggatcatct tctccttttg
     2221 gagatgcccg gcggccctcg tcaccacgga acgggtcacg ccgagcatca tcgccatgaa
     2281 ctcgtgcttc atctcgaact catccgccgg cgcgtggtcg tgcgtcatca acagccagcg
     2341 cgagaggcgc ttatccacgg cgtgcgagct gacgcaggcc gccgattgag agacctgtgt
     2401 gaagagcgcg tgggcgtagc gtcgcagcac gtgcgggagc gcgccgccgc gcacgagttc
     2461 gtctctgagc gccccggcct tcagccgcag ggcgctgccc ggcaactgga tgagcgcacg
     2521 gtgcggggcc gtattcatcc cgagcgccac cgcggtgcct aacatcccct cgttaccgat
     2581 gacgccgatc tctcttaccg tcccatcgcc cataacgact aacagcgaga tgagtccgtc
     2641 gccggggaag tagacgtgtc tgatcggctc cccgccctcg tagaggattt tcatgaacgg
     2701 cagagggacg gatttcagat gggggagcag acgctcgtac tcttcgcgcg gcagggcggc
     2761 gagaaggcgg ttccggattg gcggcggacg agaatgattg ggcatttggc gcgcgctcct
     2821 taactgtcac ccgtcgtgaa atcagcagac ggcacgagga gatgtttcgc gttcaagtcc
     2881 tgatgtcgag agatgaactg aaaggacagg tgccgcaaat atacccggtc tgagccggcg
     2941 cgtctgtgtt cttgcacaca tatcgggcgt atactcccga gtgtgaacgg cgaagggaaa
     3001 tccgttatgc cgcagagtac gcttgagccg gcggggaatc ggctgctggc caagcttccc
     3061 ccggaggaat atcagcgcct cctgcctcac ctggagcatg tctccttcgc gctcggcgag
     3121 gtcatctacg agtccggcgg gcggcagagc tacatctact tcccgaccac cgccatcatc
     3181 tctctgctct acctgatgga gaacggctcg tccgccgaga tgggcgtggc gggtcacgag
     3241 gggctggtcg gcgtcgcgct cttcatgggg ggcaataccg tccccaatcg cgccgtcgtg
     3301 cagagcgccg gcacagcctt caggatgaag acgaaggtgc ttcgggatga gttcgcgcgg
     3361 ggcggcatgt tccagcgcct gctcttgcgc tacacgcagg cgctgatgac ccagatgtca
     3421 cag
//
LOCUS       DBNGOH010000834         5018 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_308930, whole genome shotgun sequence.
ACCESSION   DBNGOH010000834 DBNGOH010000000
VERSION     DBNGOH010000834.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5018)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5018)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5018
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_308930"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..287
                     /locus_tag="AC159P_16165"
     CDS             <1..287
                     /locus_tag="AC159P_16165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697029.1"
                     /translation="DGKLFQLQDKLIPTYNTIFFQKGRNVSDPQLVALARQMGGTPVE
                     IGRDRELWAEANALAYLRERYPERGMYEPVFKEIRRTVEEYAKEHIERFR"
     gene            994..4647
                     /locus_tag="AC159P_16170"
     CDS             994..4647
                     /locus_tag="AC159P_16170"
                     /inference="COORDINATES: protein motif:HMM:NF051698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HIR3697030.1"
                     /translation="MSKNALQRGIAAMMLLLSMFVAGASAQEFRGSLAGTITDPNGAA
                     LPGVAVEIRNVETNVSNTATTNEEGGYNFPLLNPGKYTLKATAQGFNTEAREGLEVRV
                     ADKLTLDITMTVGVSETVTTVAAAETLETGTVSTGSVITSQQISELPLIDGSPYQLAT
                     LAPGVSYTGNPAFTSPTSNGNLAAFRANGATGPNQVTLDGSPNFAIDGGVGFSPPSDA
                     TQEFKVQTNAFDAQQGYSGGATVNVVIKSGTNDPHGSVYYYNRDRSRTANNFFSNRAG
                     QERPERTYHRFGGTLGGPVYIPKVYNGQDRTFFLFAYERLKNSEAEPQLFTVPTEAFR
                     RGDFSSLLTTTCTGTTTVCPTRIYDPLSAQATGTVTRAAFTGNVIPTARLNPIAVAYL
                     NLYPLPNTEGNADGTLNYFSNQIRSSNYRSWITRIDHNINDRHKIFGKYYHSFNPEDR
                     QNWTDSPLTQGFEYRTNDGGSLDYTATLSDSMVFDVRTNLSRFVQERRPAAAFDPADL
                     GFSGAALAAMNGYQYLPRFDIRTYDAGRPIRSILGASRSDYNEGLLRPFYVFSAQPSM
                     TQIWNNHTWKYGYDYRVLRENFVSNGYQGGRFFFDGTFTSVDLTTTTNSNNQTNANRN
                     RNVYGRDVAAFLLGIPSASSGQSLIDTTGINYSAQSQYHGFFFQDDWRVTPKLTLNLG
                     LRYELELGLTERYDRFISGFDDTTASPIQDAARAAYTTAFNANPSNFLLAPGDFRVTG
                     GALYADDSNRALWEADKNTWQPRVGAAYQANEKTVLRAGFGIFMSPFRIQPDDLRQTG
                     FNAQTPFIPTNDQGRTFVATLNNPFPSGFQDAFGSSRGLLTSVGQDLGASDAPLIPSD
                     RKNAKFARMIVGVQRELPGRFVVEANFVSSWGYDMAVNRNLNFVPRGFLADLSGATTL
                     AEATALDTAANTTLSATVPNPFRNLLAGTGSPFNTATTISRAQSLLRFPQFTNVWVQE
                     YDGSNRYNALQMQASKRFSQDFNLNLTYTFSKLKEKVTYLNPSDTELEDRISTDDRPH
                     RFTLAAVYQLPVGRGRLLGSDMNRFLDAVIGGWQLNGTYEWQQGQPIPLSQAFFYPGD
                     INEVRSLAGEGNGAGEKYGIRPLPVFVNPDGTPFGAGSVLRLSSSSLRTLPTTVDNLR
                     HMPFQSVNLSLTKNFRLGEGKRLQFRAEALNAFNHPYFIDLSADPTNASFGLYNTQRN
                     LPRDIQLGAKFTF"
     gene            4854..>5018
                     /locus_tag="AC159P_16175"
     CDS             4854..>5018
                     /locus_tag="AC159P_16175"
                     /inference="COORDINATES: protein motif:HMM:NF014653.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HIR3697031.1"
                     /translation="MLRVTYEELHDVLRRALLKLGFEPERAALCARLFADTDRDGVYS
                     HGLNRFPRFVR"
BASE COUNT          921 a         1901 c         1404 g          792 t
ORIGIN      
        1 ccgacggcaa gctcttccag ttgcaggaca agctcatccc cacctacaac accatcttct
       61 tccagaaggg ccgcaacgtc agcgacccgc agctcgtcgc gctcgcccgg cagatgggcg
      121 gcacccccgt cgagatcggg cgcgaccgcg agctgtgggc cgaggccaac gcgctggcct
      181 acctgcgcga gcgttacccc gaacgcggca tgtacgagcc ggtcttcaaa gagattcgcc
      241 gcaccgtcga ggagtacgcc aaagaacaca tcgagcgctt ccggtgacac cgcagtggca
      301 gtaggcagta ggcagttaaa gaaagaggct tgagacgttg ccgctcaagc ctctttcttc
      361 attgctggaa gggtgaagag gcttgggagg taaagcccca agcgcctccg ctcttctgac
      421 ttccgacttc tgacttccga cttttgtttt taccgcctac cgcctaccat ttccccttgt
      481 aatatcccca aaacggcata taattcccgc agaaaatccc gaagctgctc cgccaggaga
      541 ttcccgtcag ggcttgtccg gggagcagtt tttttacgcg gcgacggcgg cgcaggccgt
      601 cccccttctt ataccctctt cggcacctct taatcctcgc tatgtttgac gggcggcggc
      661 caaccggctc gcgggcggcg gcatctttct tcgtccgctt ttaccggaga ccggtttcgc
      721 gttcggccag cagccgaatg tgcaaataac aaagtcataa tcgtccaaga cacgaagcgc
      781 gccgccgggc gagaatgtaa ccctcggaca ccggcataca acagaggttc gcgtccggtc
      841 tccttctcag taacgcatca gacacgaatt tcagagccac ccttcgaaag gcgcgtgccg
      901 acgggcttcc ggtcgctgga cggtcgccgc cgccgccgcc gcgggtgaga accgccgggc
      961 ggccagtgtg aatatcacga aaggatcaga gtgatgagta agaacgcttt gcaacgcggc
     1021 atcgccgcca tgatgctgct gctctcgatg ttcgtcgcgg gggcgagcgc gcaggagttc
     1081 cgcggctcgc tggctggcac gatcaccgac ccgaacggcg cggcgctgcc cggcgtcgcg
     1141 gtcgagatca ggaacgtcga gaccaacgtc tcgaacaccg ccacgaccaa cgaggagggc
     1201 ggctacaact tcccgctcct gaaccccggc aagtatacgc tcaaggcgac cgctcagggc
     1261 ttcaacaccg aggcgcgcga agggcttgag gttcgcgtcg ccgacaagct caccctcgac
     1321 atcacgatga ccgtcggcgt ctccgagacg gtcaccacgg tcgccgccgc cgaaaccctg
     1381 gagaccggca ccgtcagcac cggctcggtc atcaccagcc agcagatcag cgagctgccc
     1441 ctgatcgacg gcagcccgta ccagctcgcc acgctcgcgc ccggcgtctc ctacaccggc
     1501 aacccggcgt tcaccagccc gacctcgaac ggcaacctcg cggccttccg cgccaacggc
     1561 gcgaccggcc ccaaccaggt cacgctcgac ggctcgccca acttcgccat cgacggcggc
     1621 gtcggcttct cgccgccctc ggacgcgacg caggagttca aggtgcagac caacgccttc
     1681 gacgcgcagc agggctactc gggcggcgcc accgtcaacg tcgtcatcaa gtcgggcacc
     1741 aacgacccgc acggctcggt ctactactac aaccgcgacc gcagccgcac ggccaacaac
     1801 ttcttctcga accgggcggg ccaggagcgc cccgagcgca cgtatcatcg cttcggcggc
     1861 acgctcggcg gcccggtcta catcccgaag gtctacaacg ggcaggatcg caccttcttc
     1921 ctcttcgcct acgagcgtct gaagaacagc gaggccgagc cgcagctctt caccgtgccg
     1981 accgaggcgt tccgccgcgg cgacttctct tcgctgctga cgacgacctg cacgggcacg
     2041 accaccgtct gcccgacgcg catctacgac ccgctctcgg cgcaggccac cggcaccgtc
     2101 acgcgcgccg ccttcaccgg caacgtcatc ccgaccgcgc gcctgaaccc catcgcggtc
     2161 gcgtacctga acctctaccc gctgccgaac acggagggca acgccgacgg cacgctcaac
     2221 tacttctcga accagatccg cagctcgaac taccgctcgt ggatcacgcg catcgaccac
     2281 aacatcaacg accgccacaa aatcttcggc aagtactacc acagcttcaa ccccgaagac
     2341 cgccagaact ggacggactc gcccctgacg cagggcttcg agtaccgcac caacgacggc
     2401 gggagtctcg actacacggc cacgctctcg gactcgatgg tcttcgacgt gcgcaccaac
     2461 ctcagccgct tcgtgcagga gcgccgcccc gccgccgcgt tcgaccccgc cgacctcggc
     2521 ttctcgggcg ccgcgctggc cgccatgaac ggctaccagt acctgccgcg cttcgacatc
     2581 cggacctacg acgcgggccg ccccatccgc tcgatcctgg gcgccagccg ctcggactac
     2641 aacgagggcc tcctgcgccc cttctacgtc ttctcggcgc agccctcgat gacgcagatc
     2701 tggaacaacc acacgtggaa gtacggctat gactaccgcg tgctgcgcga gaacttcgtc
     2761 agcaacggct atcagggcgg tcgcttcttc ttcgacggca cgttcaccag cgtcgatctg
     2821 acgacgacca ccaacagcaa caaccagacg aacgccaacc gcaaccgcaa cgtctacggg
     2881 cgcgacgtgg cggcgttcct cctcggcatc ccttcggcct cttcggggca gagtctgatc
     2941 gacacgaccg gcatcaacta ctcggcgcag tcgcagtacc acggcttctt cttccaggac
     3001 gactggcgcg tcaccccgaa gctcacgctc aacctcgggc tgcgctacga gctggagctg
     3061 ggcctgaccg agcgctacga ccgcttcatc agcggcttcg acgacacgac cgccagcccg
     3121 attcaggacg ccgcgcgggc cgcctacacc acggccttca acgccaaccc gtcgaacttc
     3181 ctgctcgcgc ccggcgactt ccgcgtcacg ggcggcgcgc tctacgccga cgacagcaac
     3241 cgcgcgctct gggaggcgga caagaacacc tggcagcccc gcgtcggcgc cgcctatcag
     3301 gccaacgaga agaccgtcct gcgcgccggc ttcggcatct tcatgtcgcc gttccgcatc
     3361 cagccggacg acctgcggca gaccggcttc aacgcgcaga cgcccttcat cccgaccaac
     3421 gatcaggggc gcaccttcgt cgccacgctc aacaacccgt tcccctccgg cttccaggac
     3481 gccttcggct cttcgcgcgg cctgctgacc agcgtcgggc aagacctcgg tgcctcggac
     3541 gcgccgctca tcccctcgga tcgcaagaac gcgaagttcg cgcgcatgat cgtcggcgtc
     3601 cagcgcgagc tgcccggcag gttcgtcgtc gaggccaact tcgtctcctc ttggggctac
     3661 gacatggcgg tcaaccgcaa cctcaacttc gtcccgcgcg ggtttctcgc ggacttgagc
     3721 ggcgcgacga cgctcgccga ggcgaccgcg ctcgacacgg cggccaacac cacactgtcg
     3781 gcgaccgtcc ccaacccgtt ccgcaacctg ctcgcgggca cgggcagccc cttcaacacg
     3841 gcgacgacca tctcgcgcgc gcagtcgctc ctccggttcc cgcagttcac caacgtctgg
     3901 gtgcaggagt acgacggctc gaaccgctac aacgcgctcc agatgcaggc gagcaagcgg
     3961 ttctcgcagg acttcaacct caacctgacc tacaccttct cgaagctcaa ggagaaggtg
     4021 acgtacctga acccttcgga cacggagctt gaggatcgca tcagcaccga cgaccgcccg
     4081 caccgcttca cgctggccgc cgtctaccag ttgcccgtcg ggcggggccg cctgctcggc
     4141 tccgacatga accgcttcct cgacgccgtc atcggcggct ggcagctcaa cggcacctac
     4201 gagtggcagc agggccagcc gatcccgctc tcgcaggcgt tcttctaccc cggcgacatc
     4261 aacgaggtgc ggagcctcgc cggcgagggc aacggcgcgg gcgagaagta cggcatccgg
     4321 cccctgccgg tcttcgtcaa cccggacggc accccgttcg gcgcaggctc cgtgctccgg
     4381 ctcagctcca gcagcctgcg cacgctcccg acgacggtgg acaacctgcg ccacatgccg
     4441 ttccagagcg tcaacctgtc gctcacgaag aacttccggc tcggtgaggg caagcgcctt
     4501 cagttccgcg ccgaggcgct caacgccttc aaccacccgt acttcatcga cctgagcgcc
     4561 gacccgacca acgcgagctt cggcctctac aacacgcagc gtaacctgcc gcgcgacatc
     4621 cagctcggcg ccaagttcac cttctaaaga cgacaggccg gacgccctgc cggcacggcc
     4681 ttcatcgacc aacgacaagc gagccgcccg cgccgcaagg caccgggcgg ctcgcttttt
     4741 tttacccggc cccgtgctct gttttccccg cgccccttcc gcgcgctccg cgttgaatat
     4801 tcctcacgtc acacccaacc ctcgcccgcg ccgatagcgt acaatgtcaa ctcatgctgc
     4861 gggtcactta cgaagagctg cacgacgttc tccgccgcgc gctgctcaag ctcggcttcg
     4921 agcctgagcg cgccgcactc tgcgcgcgtc tcttcgccga cacagaccgc gatggcgtct
     4981 attcgcacgg cctcaaccgc ttcccgcgct tcgtccgg
//
LOCUS       DBNGOH010000835         2190 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3606932, whole genome shotgun sequence.
ACCESSION   DBNGOH010000835 DBNGOH010000000
VERSION     DBNGOH010000835.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2190)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2190
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3606932"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2036
                     /locus_tag="AC159P_16180"
     CDS             <1..2036
                     /locus_tag="AC159P_16180"
                     /inference="COORDINATES: protein motif:HMM:NF051698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HIR3697032.1"
                     /translation="SSWRRQPSWADQSIIGLTSVFGNSLVNDLRFSYFYVNSSVLPVT
                     EQECPGCLGVGSPAITVSQTGLSLGVSVTQLTHARRYHLTDYATWQRGEHRLRFGVDW
                     EYNRGGALVANDEPATLVLHPPQRARASGTPVPSAFRTLDDILQLPLQSVTVGVGDPR
                     VPQADGGLVRNWHTLRLFFQDTWRLRPRLSVNYGLGWSIDRNLNHDLSKPALLAPILG
                     ADGLGPTRKQWKNFSPVLGVAWSPARDAKTVVRAGAGLFYDFLFPPFLDNERALLGPP
                     GPGRQTISGNRLFNCLPGIPGVPVGAALNFNTPTRFTGANLLTCLPTIRAELTRNLAN
                     ADQSIQAIQLSKQAAQGAALNPVDVPSWSALHVNLGVQREVAREFVVSADFAYRHFDH
                     IGLGQLDLNHFNSARGPVIPRCLNETQRNDPTAHCSTGPINVQVNAGRATYKGLLVRA
                     DKRFSRGFQLLGSWAYSSNTGTNSGNGGTGNGFNLDDWLANRGALDRDVTHIVNVAGV
                     APLPFGFRLGLNFSYASAPPFSAFVGGSDFNGDGTTDDLLPGTTVNAFNRSLGRAELE
                     RLVAEFNRTHAGRVDASRVGVLIPRLTLPERYWFGDNFQSLDLRLSRSFDFEKGWRLT
                     LVGEAFNLYNAANLSGHSGNLAGIGFGQPTARFTQVFGSGGPRAFQLGARVSF"
BASE COUNT          357 a          770 c          688 g          375 t
ORIGIN      
        1 cttcgtcgtg gaggcgacaa ccgtcctggg ccgaccagag catcatcggt ctcaccagcg
       61 tcttcggcaa ctcgctggtc aacgacctcc gcttctcgta cttctacgtc aactccagcg
      121 tgttgccggt caccgagcag gagtgccccg ggtgcctcgg agtcggctcg cctgcgatca
      181 ccgtgtcgca gaccggcctc tctctcggag tctccgtgac gcaactcacg cacgcccggc
      241 gctaccacct caccgactac gcgacctggc agcgtgggga gcaccgtctg cggttcggcg
      301 tggactggga atacaaccgc ggcggcgcgc tcgtcgcgaa cgacgagccg gcgacgctcg
      361 tgctccatcc gccgcagcgt gcgcgggcat ccggcacccc cgtgccctcc gcgtttcgca
      421 cgctcgacga catccttcag cttccgttgc agagcgtcac cgtcggcgtc ggcgacccgc
      481 gcgtgccgca ggcggacggc ggcctggtgc gcaactggca cacgctgcga ctcttctttc
      541 aagacacctg gcggctgcgc ccgcggctga gcgtcaacta cgggctgggc tggagcattg
      601 atcgtaatct caaccacgac ctttcgaagc ccgcgctgct cgcgcccatc ctgggcgcgg
      661 acggcctcgg gccgacgcgc aaacagtgga agaacttctc gcccgtgctg ggggtagctt
      721 ggtcaccggc ccgtgacgcg aagaccgtgg tccgtgcggg cgccggcctc ttctacgatt
      781 tcctgttccc gcccttcctc gacaacgagc gcgcgctgct cggaccgccc ggtccgggcc
      841 gtcagacgat ctccggcaac aggctcttca actgcctgcc gggcatcccg ggtgtcccgg
      901 tcggggcggc gctcaacttc aacacgccga cgcggttcac cggcgcgaac ctgctgacct
      961 gcctgccgac gattcgggcc gagctgacac gcaacctcgc caatgcggat cagtccattc
     1021 aggcgatcca gctcagcaag caggccgcgc agggcgccgc cctgaacccc gtcgatgttc
     1081 cgagttggtc ggcgctccac gtcaacctcg gcgtccaacg cgaagtcgcg cgcgagttcg
     1141 tcgtgagcgc cgacttcgct taccgccact tcgaccacat cggtctgggg cagctcgacc
     1201 tcaaccattt caacagcgcg cgcggccccg tcatcccgcg ctgcctgaac gagacacagc
     1261 gcaacgaccc cacggcgcat tgctcgacgg ggccgatcaa cgtacaggtc aacgcggggc
     1321 gcgccaccta caaggggctg ctcgtgcgcg ccgacaagcg cttctcgcgc ggcttccagt
     1381 tgctcggctc gtgggcctat tcgagcaaca ccggaaccaa cagcgggaac ggcggcaccg
     1441 ggaacggctt caacctggac gactggctcg ccaaccgtgg cgcgctcgac cgcgacgtga
     1501 cgcacatcgt caacgtggcg ggcgtcgcgc cgctgccgtt cggcttccgg ctcgggctga
     1561 acttctcgta cgcgagcgcc ccgcccttca gcgccttcgt cggcgggagc gacttcaacg
     1621 gcgacggcac gacggacgac ctgctgcccg gcaccaccgt caacgccttc aaccgctcgc
     1681 tggggcgtgc ggagttggag cggctcgtcg ccgagttcaa ccggacccac gccggcaggg
     1741 tggacgccag cagggtcggc gtgctgatcc cccggctgac gctgccggag cgttactggt
     1801 tcggcgacaa cttccagtcg ctcgacctgc ggctgagccg ttcgttcgac ttcgagaagg
     1861 ggtggcggct gacactcgtc ggcgaggcgt tcaacctcta caacgcggcg aacctgtcgg
     1921 ggcacagcgg caacttggcg ggcatcgggt tcgggcagcc gacggcgcgg ttcacgcagg
     1981 tcttcggctc gggcgggccg cgcgccttcc agttgggagc gcgggtcagt ttttgagtcg
     2041 gttaaggacg ataggtcttt tagagcctga ataattattt cagacttgcg ttaagacctg
     2101 gaaagggcct ggcaaacatc cctgcttgcc tagcatttgc aaaacttact tctaaatagc
     2161 taaaagagga atgatagaag accggtcatc
//
LOCUS       DBNGOH010000836         2284 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1227001, whole genome shotgun sequence.
ACCESSION   DBNGOH010000836 DBNGOH010000000
VERSION     DBNGOH010000836.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2284)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2284)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2284
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1227001"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..545)
                     /locus_tag="AC159P_16185"
     CDS             complement(<1..545)
                     /locus_tag="AC159P_16185"
                     /inference="COORDINATES: protein motif:HMM:NF024863.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HIR3697033.1"
                     /translation="MELPVMNVKTYALVWPLLLGLAYGSASAQSNKVERQRRFEPEIK
                     RFEEADKQQPPAEGAVLFIGSSSIRLWKSLAEDFPSVKVVNRGFGGSHIEDSVDFADR
                     IIFRHKPRLIVMYAGDNDIESGKSPAVVLADFEKFVAAVHRKLPRTRIAFISIKPSPA
                     RWHLVDKAREANRSIKDFTET"
     gene            complement(596..1669)
                     /locus_tag="AC159P_16190"
     CDS             complement(596..1669)
                     /locus_tag="AC159P_16190"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HIR3697034.1"
                     /translation="MRRTPPSVRHTPLAALCVFAFGVTLVAPPPRAVTRGAAPPSQPR
                     EQEALPRPAPRWYKGNTHTHTLNSDGDSTPDEVVRWYREHGYHFLALTDHNYLTRVDA
                     LNALHGADEKFLVIKGEEVTDRHGEKSIHINGLDVERVVAPQGGAGVADILQRNVDAI
                     RAARGVPHINHPNFTWAITAEDLKRVRGDKLFESYNGHPLVNNAGGGGRPGLEELWDE
                     ILSSGKLLYGIAVDDAHHFKRPWDRTAALPGRGWVMVRAEALTTAAILGALERGDFYA
                     STGVELADYQADAGGVAVKVKEVASSKYRVRFIGKGGRVLKEVADASATYRFTGEEGY
                     VRAKVLDSNGWAAWTQPLPVRRP"
     gene            complement(1770..>2284)
                     /locus_tag="AC159P_16195"
     CDS             complement(1770..>2284)
                     /locus_tag="AC159P_16195"
                     /inference="COORDINATES: protein motif:HMM:NF051698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HIR3697035.1"
                     /translation="RALLDGWQVNSIVTAHTGFPWTPLTRRPELIAPGGRRLSPIRPI
                     AYLGGDLRGDTSDEAFTRAQGNFPGGGERYFVITGSVQRPGIGRNSFRGPNYFNVDLS
                     LVKRTYMPFIKEGAYLDLRANFFNAFNLLNLQNFAFGGAGTIIEDANFGRTPGGTAGR
                     VVELQARFSF"
BASE COUNT          342 a          744 c          814 g          384 t
ORIGIN      
        1 tcggtctcgg taaaatcctt tatcgaacgg ttggcttcgc gcgccttgtc caccagatgc
       61 catcgcgccg ggctgggctt gatggagatg aaggcgatgc gcgtgcgggg caacttccga
      121 tgaaccgcgg cgacgaactt ttcgaaatcc gccagcacga ccgccggact cttcccgctc
      181 tcgatgtcgt tatcgcccgc gtacatgacg atcaggcggg gcttgtggcg aaagatgatg
      241 cggtcggcga aatcgaccga gtcttcgatg tgcgagccgc cgaacccgcg gttgacgact
      301 ttgaccgacg ggaaatcttc ggccaggctc ttccacagtc tgatcgagga gctgccgatg
      361 aagagtacgg cgccttcggc gggcggctgc tgcttgtcgg cctcctcgaa ccttttgatc
      421 tcaggctcga accggcgttg ccgctcgacc ttgttcgact gggcggaggc cgagccgtag
      481 gcgaggccga gcaggagcgg ccagaccagc gcgtaagtct tgacgttcat cacgggcagc
      541 tccattgtcg gggccggcgc acggagcgcg aaccgccccg gcggactcca gcgggctacg
      601 gtcggcgaac cggcaacggc tgcgtccagg ccgcccaccc gttcgagtcc agcaccttgg
      661 cgcggacgta gccctcttcg ccggtgaacc ggtaagtcgc ggaggcgtcc gccacctcct
      721 tcaggacgcg cccgcctttg ccgatgaacc ggacgcggta cttgctcgac gcgacttcct
      781 tgaccttgac cgccacgccc cctgcgtccg cctgatagtc ggccagctcg acgccggtcg
      841 aggcgtagaa gtcgccgcgt tcgagcgcgc cgaggatcgc ggcggtcgtc agcgcctcgg
      901 cccgcaccat gacccacccg cgaccgggga gcgcggcggt tctgtcccac ggcctcttga
      961 agtggtgcgc gtcgtcaacg gcgatgccgt agagcagctt gccgctcgac agaatctcgt
     1021 cccacagctc ttcgaggccg gggcggcctc ccccgcccgc gttgttgacg agcggatggc
     1081 cgttataact ctcgaacagc ttgtcgccgc gcacgcgctt caggtcttcc gccgtgatcg
     1141 cccacgtgaa gttcgggtgg ttgatgtgcg ggacgccgcg cgccgcgcgg atggcgtcaa
     1201 cgttgcgctg gaggatgtcc gccacgccgg cgcccccctg cggcgcgacg acgcgctcca
     1261 cgtcgaggcc gttgatgtgg atggacttct cgccgtgacg gtcggtcacc tcttcgccct
     1321 tgatgacgag gaacttctcg tcggcgccgt gcagcgcgtt gagcgcgtcc acacgcgtca
     1381 ggtagttgtg gtcggtgagg gcgaggaagt gatagccgtg ctcgcgatac cagcgcacga
     1441 cctcgtcggg cgtggagtcg ccgtcgctgt tcagggtgtg cgtgtgcgtg ttgcccttgt
     1501 accaacgcgg ggcgggccgc ggcagggcct cctgctcacg cggctgactc ggcggcgccg
     1561 cgccccgggt cacggcccgc ggcggcgggg cgacgagcgt cacgccgaac gcgaagacgc
     1621 acagggcggc gagcggcgtg tgccgaaccg aaggcggggt gcgacgcata ggcgatgcgg
     1681 ggcttgcggc ctctgtcgga aagcgagggg cgggcaatcg ccgacgaagt ttgcccgccc
     1741 cttcggacgc acacgatttc cggggcgcgc tagaagctga agcgcgcctg aagctcgacg
     1801 acgcgcccgg cggtgccgcc cggcgtgcgc ccgaagttcg cgtcctcgat gatggtgccc
     1861 gccccgccga acgcgaaatt ctgaaggttc agcaggttga aggcgttgaa gaagttggcc
     1921 cgcagatcga ggtacgcccc ctccttgatg aagggcatat aggtgcgctt gacgaggctc
     1981 agatcgacgt tgaagtagtt ggggccgcgg aaactattgc gcccgatgcc ggggcgctgg
     2041 acgctgccgg tgatgacgaa gtagcgctcg ccgccgccgg ggaagttccc ctgcgcgcgg
     2101 gtgaaggctt cgtccgacgt gtcgcctctg aggtcgccgc cgaggtaggc gatcgggcgg
     2161 atcggcgaga ggcggcggcc gccgggcgcg atcagctcgg gccgccgcgt cagcggggtc
     2221 caggggaagc ccgtgtgcgc ggtgacgatg ctgttgacct gccagccgtc gagcagcgcg
     2281 cggg
//
LOCUS       DBNGOH010000837         2225 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4707536, whole genome shotgun sequence.
ACCESSION   DBNGOH010000837 DBNGOH010000000
VERSION     DBNGOH010000837.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2225)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2225)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2225
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4707536"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="AC159P_16200"
     CDS             <1..193
                     /locus_tag="AC159P_16200"
                     /inference="COORDINATES: protein motif:HMM:NF013227.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HIR3697036.1"
                     /translation="VETLIHYPYLLHRQTLFTRQRAHPPLPTAERAGAQILSLPLYPH
                     LRDDEVRTVIDAVLEFAGK"
     gene            complement(214..1035)
                     /locus_tag="AC159P_16205"
     CDS             complement(214..1035)
                     /locus_tag="AC159P_16205"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3697037.1"
                     /translation="MQEEDYAYLYELEESFWWFAGMREVTAALLDPLCTEGEGARAAL
                     DAGCGTGDMLTRLSRYAPAESIVGIDVSRTALDFCRERGHARLAQASATHLPFAAESF
                     DLVTSFDVLVQIPGEGADELAVREMFRVLRPGGVAFVRVAAYEWMKSGHDAALGTQRR
                     YTLGELNAKMERAGFRVLRATYANSFLLPVAALRRLLLKRLGLADAGSDVKPLPPSLR
                     RLNGLLTGVLRTEARMLKGARTTLPFGLSAICVAQKPATEPFRTAGDGALTPNSR"
     gene            1395..>2225
                     /locus_tag="AC159P_16210"
     CDS             1395..>2225
                     /locus_tag="AC159P_16210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697038.1"
                     /translation="MVKRIIAIIFIFLCASVAWGILGATIFSRTYSADGTLKDRVVSI
                     WGAPHRQTPPTATFEKIVEKPNATTVNGQQVFGMVRERVATPLPLDSSRVDVALSLEH
                     RQKGLLWYSTYKVAFAGVYTFRNAGDKPEEVTFTLNFPTGQAIYDDLVFTVNDAPVVL
                     TSEQRATHGTALLAPGQTATLRVGYRSQGLDEWRYGFAAGEVAQVKDFRLTMKTDFRE
                     VDFPENTLSPSEKSETADGWQLVWDYKNLVSGYEIGMTMPEKLQPGPLAGRISLFAPV
                     S"
BASE COUNT          401 a          759 c          710 g          355 t
ORIGIN      
        1 cgtcgagaca ctgattcact acccctacct gctccaccga caaacactct tcacgcgcca
       61 acgcgcacac cccccgctcc ccaccgccga acgcgccggc gcccaaatcc tctcgctccc
      121 gctctacccg cacctccgcg acgacgaagt tcgaacggtc atcgacgccg tgctggaatt
      181 cgccgggaaa taaacggcgc gcgcagccac agattagcgt gagttcggcg taagcgctcc
      241 gtcgcctgct gtgcggaaag gctccgtggc gggcttttgc gcgacgcaga tggcggagag
      301 gccgaagggg agtgtcgtgc gcgcgccttt gagcatacgc gcttcggtgc ggagcacgcc
      361 ggtcaagagg ccgttgagcc gtcggaggct cggcgggagc ggcttcacgt cggagcctgc
      421 gtcggcgagg ccgagtcgct tcagcagcag tcgccgcagg gccgcgaccg ggagcagaaa
      481 gctgttggcg taggtcgcgc ggaggacgcg gaagcccgcg cgctccatct tcgcgttcag
      541 ctcgccgagc gtgtaacgcc gctgcgtgcc gagcgcggcg tcgtggccgc tcttcatcca
      601 ttcataagcg gcgacgcgga cgaacgcgac gccgccgggc cgcagcacgc ggaacatctc
      661 gcgcacggcc agctcgtccg cgccttcgcc cggtatctgc acgagcacgt cgaagctggt
      721 gacgaggtcg aaggactccg cggcgaaggg caggtgcgtc gccgaagcct gcgcgagccg
      781 cgcgtgcccg cgctcgcggc agaagtcgag cgcggtgcgc gacacgtcga tgccgacgat
      841 gctctcggcg ggcgcgtaac ggctgagtcg cgtcagcatg tcgcccgtcc cgcaccccgc
      901 gtcgagcgcg gcgcgcgcgc cctcgccctc cgtgcacaaa gggtcgagca gcgccgccgt
      961 cacttcgcgc atccccgcga accaccagaa gctctcttcc aactcgtaga ggtacgcgta
     1021 gtcttcctct tgcataatga atcggtaggc agtaggcggt aggcaagcaa aaacagaaac
     1081 agaaaacaga aaacagacag cagttagcgg tcggaggaat ctcggattcg ggattcgagt
     1141 ttcaagtcgg aaattgtatc gactgcctac cgcctaccgc ctaccgccta cttctttctc
     1201 atcttatcac cgaccgcgct cggtctcggc gtctccgaaa tgtcgtgtgg ctgcgtctgc
     1261 tcacgaccct ccactgtggg aagccacatt gccgcggttc gcctccgggc taagaatcac
     1321 accgtcacga aaccgagggg cggcgcggcc acggcggcca cggcgttctt tcaacgtaag
     1381 gagcggcacg cgtcatggtc aagcgcatca tcgccatcat cttcatcttc ctctgcgcct
     1441 ctgtggcgtg gggcatcctc ggcgcgacga tcttttcgcg cacctactct gcggacggca
     1501 cgctcaaaga ccgcgtcgtc tcgatctggg gcgcgccgca ccggcagacg ccgcccaccg
     1561 cgaccttcga gaagatcgtc gagaaaccga acgcgaccac cgtcaacggc cagcaggtct
     1621 tcgggatggt gagggagaga gtggcgacgc cgctcccgct cgacagcagc cgcgtggacg
     1681 tggcgctctc tctggagcac aggcaaaagg gtctgctgtg gtacagcacc tacaaggtcg
     1741 cgttcgcggg cgtctataca ttccgcaatg cgggcgacaa gcccgaggag gtgacgttca
     1801 cgctcaactt cccgaccggg caagccatct acgacgacct cgtcttcacg gtcaacgacg
     1861 cgcccgtcgt gctcaccagc gagcagcgcg cgacgcacgg cacggcgctg ctcgcgccgg
     1921 ggcagacggc gacgctccgt gtcggctacc gctcgcaagg gctggacgag tggcgctacg
     1981 gcttcgccgc gggcgaggtg gcgcaggtga aagacttccg gctcacgatg aagacggact
     2041 tccgtgaagt ggactttccg gagaacacgc tctcgccttc ggagaagtcg gagaccgccg
     2101 acggctggca gctcgtctgg gactacaaga acctcgtctc cggttacgag atcgggatga
     2161 cgatgcccga gaagttgcag cccggcccgc tcgccgggcg catcagcctc ttcgcgcccg
     2221 tctcg
//
LOCUS       DBNGOH010000838         3676 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3791357, whole genome shotgun sequence.
ACCESSION   DBNGOH010000838 DBNGOH010000000
VERSION     DBNGOH010000838.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3676)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3676)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3676
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3791357"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(19..936)
                     /locus_tag="AC159P_16215"
     CDS             complement(19..936)
                     /locus_tag="AC159P_16215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013787486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR3697039.1"
                     /translation="MDTFVQQLINGLTIGSIYALIALGYTMVYGILRLINFAHGDIYM
                     LGAFSGYFLARGLGFAQEPSLFGLGVTLLGAMIIAALIGMAIERFAYRPVRRYARMTT
                     LITAIGVSLLLENLGVVIFGGAPRSFPELLQNETYTLFGNAAISKSQLLIFGVSIALM
                     LLLQWIVYRTKVGTAMRAVSFNLNSAKLMGINTDFIIVFTFALGSALAAAGGVLTAQY
                     NPTIYPLMGIITGLKAFVAAVLGGIGNIPGAVLGGLLIGAAETFVVGYGQLVGIPSTY
                     RDAVAFAILILVLLFRPAGLLGTTVQEKV"
     gene            complement(983..2206)
                     /locus_tag="AC159P_16220"
     CDS             complement(983..2206)
                     /locus_tag="AC159P_16220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR3697040.1"
                     /translation="MKRALLSLVLVAAVFSSFACERRGTGGTNGGGDTGAIKVGIYGD
                     LSGQTSSFGQSTKNGALMAIDEINARGGINGRKVEYVIEDDQGQPAQAATVVSKLINQ
                     DKVHAILGEVASTNSLAAAPVAQSAKIPMITPSSTNPKVTQVGDYIFRVCFTDDFQGA
                     VAATFAGNTLKAKTAAVLGDFQSDYSKGMSQFFEAEFARLGGRIIQKQTYTQTDSDFK
                     GQLTSIRSANPDVIFVPGYYGQVGVIAKQAKELGIKAPLLGGDGWDAPQLWDLGGSAL
                     DGAYMVNHYSVDDPSPAVKKFVADYKTKYSGVAPDAIAALAYDAMNVLADAIKRAGTT
                     DGTKLRDAISQTKDFLGVTGSISINAQRDAVKPAVIFELRDKKLVYKETIQPSGGSAA
                     PSASPAAHSNTNASY"
     gene            2834..3580
                     /locus_tag="AC159P_16225"
     CDS             2834..3580
                     /locus_tag="AC159P_16225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697041.1"
                     /translation="MADYKKKDEGITEEASAFGQRVKGAAKDAAGSITGNERLEREGE
                     RENAIGDARQEANDMFPNTRAAAADATTGAATGTARSRTLSGLFRDRESAENAYRSLT
                     DRGYTRDDVNVVMSEDTRKTHFADDDSELGSKALEGMGVGGAVGGTVGALLAAIAAIG
                     TSVLLPGLGLVVAGPLAAALAGAGAGSVTGGLIGALVGSGIPEERAKYYDEGVRNGGI
                     YMGVNPRTDEDAEYFENEWKTNYRGEHVYR"
BASE COUNT          644 a         1144 c         1293 g          595 t
ORIGIN      
        1 cagcacccag cacccctttc agactttctc ctgcaccgtc gtccccagaa ggccggcggg
       61 gcggaagagc aggacgagaa tcaggatggc gaaggcgacg gcgtcgcggt acgtcgaggg
      121 gatgccgacg agctggccgt agccgacgac gaaggtctcg gccgcgccga tgagcagccc
      181 gcccaagacc gcgccgggga tgttgccgat gccgcccagc accgcggcga cgaaagcctt
      241 gaggcccgtg atgatgccca tcagcgggta gatggtcggg ttgtactgcg cggtcaggac
      301 gccgcccgcc gccgccagcg ccgaccccaa agcgaaggtg aagacgatga tgaagtcggt
      361 gttgatgccc atcagcttcg ccgagttgag gttgaacgag acggcgcgca tcgcggtgcc
      421 gaccttcgtg cggtagacga tccattgcag caggagcatc agcgcgatgg agacgccgaa
      481 gatcaggagc tgcgatttcg agatggcggc gttgccgaag agcgtgtagg tctcgttctg
      541 caaaagctcc ggaaaagagc gcggcgcgcc gccgaagatg acgacgccga ggttctccag
      601 cagcagcgag acgccgatgg ccgtgatgag cgtcgtcatc cgcgcgtagc ggcgcacggg
      661 ccggtaggcg aaacgctcga tcgccatccc gatcagcgcc gcgatgatca tcgcgcccaa
      721 gagcgtcacg cccagcccga agagcgacgg ctcctgcgcg aagccgagac cgcgcgcgag
      781 gaagtagccg ctgaaggcgc cgagcatgta gatgtcgccg tgggcgaagt tgatgagccg
      841 cagaatgccg tagaccatcg tgtatccgag cgcgatgagc gcgtagatcg agccgatggt
      901 caggccgttg atgagttgct gaacgaaggt gtccatgcga tgcgctcggt cgagtgttgg
      961 tgatgcgtgt cagtggttcg cgtcagtagc tcgcgttcgt gttcgagtgg gccgcgggcg
     1021 aggccgaagg cgccgcgctg ccccccgagg gctggatcgt ctctttgtag acgagcttct
     1081 tgtcgcgcag ctcgaagatg acggcgggct tcacggcgtc gcgctgcgcg ttgatgctga
     1141 tcgagccggt gacgccgagg aagtccttcg tctgcgagat ggcgtcgcgc agcttcgtgc
     1201 cgtcggtcgt gcccgcgcgc ttgatggcgt cggccaaaac gttcatcgcg tcgtaggcga
     1261 gcgcggcgat ggcgtcgggc gcgacgccgc tgtacttcgt cttgtagtcg gcgacgaact
     1321 tcttgacggc ggggctgggg tcgtcaaccg agtagtggtt gaccatgtag gcgccgtcga
     1381 gcgccgagcc gccgaggtcc cagagctgcg gcgcgtccca gccgtcgccg cccaggagcg
     1441 gcgccttgat gccgagttcc ttcgcctgct tggcgatgac gccgacctgc ccgtagtagc
     1501 cggggacgaa aatcacgtcc gggttggcgg agcggatcga ggtcaactgg cccttgaagt
     1561 ccgagtcggt ctgcgtgtag gtctgcttct ggatgatgcg cccgccgagg cgcgcgaact
     1621 cggcctcgaa gaactgcgac atgcccttgc tgtagtcgga ctggaagtcg cccaggacag
     1681 ccgccgtctt ggccttcagg gtgttgccgg cgaacgtcgc ggcgaccgcg ccctgaaagt
     1741 cgtccgtgaa gcagacgcgg aagatgtagt cgccgacctg cgtcaccttc gggttggtcg
     1801 aggagggcgt gatcatcgga atcttcgccg actgcgcgac cggcgcggcg gccagactgt
     1861 tggtcgaagc gacctcgccg aggatggcgt gcaccttgtc ctgattgatg agcttggaga
     1921 cgacggtcgc ggcctgcgcg ggctggccct gatcgtcctc gatgacatac tcgaccttgc
     1981 gcccgttgat gccgccacgg gcgttgatct cgtcaatcgc catcagcgcg ccgttcttcg
     2041 tcgattggcc gaagctcgaa gtctggcccg acaggtcgcc gtagatgccg accttgatcg
     2101 cgccggtgtc gccgccgccg ttcgtgccgc ccgtgccgcg acgctcgcag gcgaaagaag
     2161 agaagaccgc ggcgacgagg acgagtgata gcaaagctcg cttcatgtta aacctcacag
     2221 gatggatttt ttgaacgggg cgacaggcgg cgggagactt cggtaaaaac cgccgtcgcg
     2281 cgtgaaccga accgatggcc cttgtgtgtt cgtgccgcga cgcgcgcggc gagcgggact
     2341 tgggcgccgc cgctttcgtg cccggcgggc ggacgcaggt gcgcctgaac tgccccggcg
     2401 tcgctttcat cctcggcgct ggtgcgcgcg cgcagtccgg gatgtctcat ttcttcggct
     2461 cttggtcagt aatatggcgg cgggccgcat gccccgtcaa gcccacgccg cgcggtgtgt
     2521 aaagcggacg cgcgcattat accgttttcg cgcgacctgt cgagtgcccg cacgctgttc
     2581 gaggagttcg gacacgcagg cacgcgcgcg ggggttgagt gcggcacggt gcttgcccct
     2641 acagtctacg tggccagttt gaagacgtaa aacgatacac cccgcgcgcg acagagtacg
     2701 ctcggcgggg gctaacaagg ggtatgcgtg gcggcgggcg ggcatgtctc tcgcatctgt
     2761 ccggccacgg cggtaagcaa ggtgaagcac ggcgcttcag gttcataact aatacacgaa
     2821 agggagaact gagatggcag attacaagaa gaaggatgaa ggcatcaccg aagaggcttc
     2881 ggcgttcgga cagcgcgtga agggcgcagc gaaggacgcc gccggttcga tcaccggcaa
     2941 cgaaagactt gagcgcgaag gtgagcgcga gaacgccatc ggcgacgcac ggcaagaggc
     3001 caacgacatg ttcccgaaca cgcgcgccgc tgccgccgac gccaccacgg gcgcggccac
     3061 cggcacggcc cgcagccgca ccttgagcgg tctgttccgc gaccgcgaga gcgccgagaa
     3121 cgcctaccgc tcgctgacgg atcgcggcta cacgcgcgac gacgtgaacg tcgtcatgtc
     3181 ggaagacacg cgcaagacgc acttcgctga cgacgactcg gagctgggca gcaaggcgct
     3241 cgaaggcatg ggcgtcggcg gcgccgtcgg cggcaccgtg ggcgctttgc tcgcggccat
     3301 cgcggccatc ggcacgagcg tgctgctgcc gggcctcggc ctcgtcgtcg ccggcccgct
     3361 cgcggcggcg ctggcaggcg cgggcgcggg cagcgtgacg ggcggcctca tcggcgcgct
     3421 cgtcggctcg ggcatccccg aggagcgcgc gaagtactac gacgaaggcg tccgcaacgg
     3481 cggcatctac atgggcgtca acccgcgcac ggacgaggac gccgagtact tcgagaacga
     3541 gtggaagacc aattaccgcg gcgagcacgt ctaccgctaa acgcggcatc ctagctcgat
     3601 tcgccaaagg ggaggcaact ttcgttgcct cccctttttt ccatccccgc ccgcatcggc
     3661 agacgccgcg ccgact
//
LOCUS       DBNGOH010000839         4156 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3607374, whole genome shotgun sequence.
ACCESSION   DBNGOH010000839 DBNGOH010000000
VERSION     DBNGOH010000839.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4156)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4156)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4156
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3607374"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..945)
                     /locus_tag="AC159P_16230"
     CDS             complement(<1..945)
                     /locus_tag="AC159P_16230"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HIR3697042.1"
                     /translation="MTAVEKTGKRPERLKFEGRVLFLTEDVSLVRRQLEGGEELEFDP
                     ERALMNNISTDEITPGWVCFYYDETVGEYVYVGMREGAVKKDEVKQGGFGVVVSGLSK
                     GCGSSRETAPFAEKAAGLQLVIAKTIEKIYGQNCQNIGLLTSTDFGLIERIRNGEEIP
                     LAEFTRGLDPISQSIVEYGGLFNYNKARLAGETEPPALETSKRPLNIVEKIIARHAFV
                     RAGEVGVEAVRPGDALFAVADVRFSHEYVTPMAESLFKQALGKDARVTEAESVYAFRD
                     HLTFLGRVMPEEKRKMGLLERADGLAVTQAAFTEEQGIR"
     gene            1174..2043
                     /locus_tag="AC159P_16235"
     CDS             1174..2043
                     /locus_tag="AC159P_16235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697043.1"
                     /translation="MKIIACLLALTVLAPLAAAARQKAADATPIELCSARLVRYADWP
                     ALKDPTFSLELTAASGGRLRYFGAAHSADPADAQFAEIEKAWDALKPTVAFYEGPNRP
                     VAASAEETIRQAGESGFVRFLAKRDGVAFLTLEPSPQEEAAFVSKKYPAEQVMLFYVL
                     RETARLRERRKLPEAELKTAIAGLLEKASKMTGLAPFTTLEGLEAAYRRHWTTPANWW
                     EAPQQWFDPLKSSQETGGLFTNDINRLSSEYRNRHMYTALASAALEGQRVFAVVGRNH
                     VPMQAQALRCAVK"
     gene            2183..2353
                     /locus_tag="AC159P_16240"
     CDS             2183..2353
                     /locus_tag="AC159P_16240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697044.1"
                     /translation="MAEQLKADGGVEVETVKGGFGEFSVHLDGRKIVETSRLWYPTPR
                     KVVEQVRAALAA"
     gene            2437..3825
                     /locus_tag="AC159P_16245"
     CDS             2437..3825
                     /locus_tag="AC159P_16245"
                     /inference="COORDINATES: protein motif:HMM:NF025473.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABA4-like family protein"
                     /protein_id="HIR3697045.1"
                     /translation="MYVTLFNLAGYAIVAWLLLVFLPKWSVTRFVARTAIFPVYLSVL
                     YVVGVAAVLAELGPGLMRDFGSAEGVTRILARQDVALVAWIHILAFDQLVALHIYREN
                     MDHHYVPLPVQSVLLVLTLMFGPVGFLAYYVLRLVSRSRGGRRVGEGEAAAANDGQHD
                     PRRAETAARVSVTGRGLLSVFDEERALFRTALAGIVLGTLLFIYIGVNGRAVPPEGDL
                     FKAATFDIAVGIYVLTVTLLLPFAGFSPRGRAVWRWLTVAFMSYGYALETVQMLRGLD
                     PRFTRAGGPADQLLGGVFFLVALGLAVLFVVLALRFFSSRTAVVGTLFLLGMRYACAA
                     ALFAHGVGLWMSLNQGPRIGAGGNLLPLHAAGFHGLQAVPLVALLFAWSQTPTEAARR
                     WVHAAGLAWLGACLAIAWQTARGGAVLEPSSPTALAAALFAVWAVAFARAAFARRRAG
                     IEDYSQRAGAAA"
BASE COUNT          672 a         1436 c         1280 g          768 t
ORIGIN      
        1 ccgtatgccc tgctcctccg tgaaggcggc ttgcgtcacc gcgagtccgt cagcgcgttc
       61 cagcaggccc atcttccgct tctcttccgg catcacgcgc cccaggaaag tcaggtggtc
      121 gcggaacgcg tagaccgact cggcctccgt cacgcgcgcg tctttgccga gagcctgctt
      181 gaacagactc tcggccatcg gcgtcacgta ctcgtgcgag aagcgcacgt ccgccaccgc
      241 gaacagcgcg tcgcccggcc tgaccgcctc gacgcccacc tcgccggccc tcacgaacgc
      301 gtgccgcgcg atgatcttct ccacgatgtt caacgggcgc ttcgacgttt cgagcgcggg
      361 cggctccgtc tcgcccgcga gccgcgcctt gttgtagttg aacagcccgc cgtactcgac
      421 gattgattgc gagatagggt caagcccgcg cgtgaactcc gccagcggta tctcctcgcc
      481 gtttcggatg cgctcgatca ggccgaagtc cgtcgaagtc agcagcccta tgttctgaca
      541 gttctgcccg taaatcttct cgatcgtctt cgcgatgacg agttgcagcc ccgccgcctt
      601 ctccgcgaac ggcgccgtct ccctcgaaga cccgcacccc ttcgacaacc ccgacaccac
      661 caccccgaac ccgccctgct tcacttcatc cttcttgacc gccccctccc gcatccccac
      721 atacacatac tcccccaccg tctcgtcgta ataaaaacag acccaccccg gcgtgatctc
      781 gtccgtcgag atgttattca tcagagcgcg ctccgggtcg aactccaact cctcgccccc
      841 ttcgagctgc cgccggacga gcgacacgtc ctccgtcaaa aacaacaccc gcccctcgaa
      901 cttcaaacgc tcggggcgct tccccgtctt ctcaacagcg gtcataactt ctcctgtgtg
      961 gatatcatcg cggcttgaat taagaagaga ttataaaccg gacgggcgcc aaacgccgaa
     1021 cacggcgccg cgcgaaaagc ctgccaaacc ggcgggcgcg gcgtatgatg cgagtcatta
     1081 gtcggaaccg atgactcgat ggcggaaccg tggtggggcg gggcgcgtac atgacaccgt
     1141 aattccatcg actgtgaaga ggtgtcggaa cgcttgaaga taatcgcttg cctgttggct
     1201 ttgacagtcc tcgcgcccct cgccgccgcg gcgcggcaga aggcggcgga cgcgactccc
     1261 atcgaactct gctccgcgcg tctcgtccgc tacgcggact ggccggcgtt gaaagaccct
     1321 acgttctctc tggagttgac ggcggcctcg ggcgggcggc ttcgctactt cggcgcggcg
     1381 cattcggcag accctgcgga cgcacagttc gcggagatcg aaaaagcgtg ggacgcgctg
     1441 aaaccgaccg tcgcttttta cgaggggccg aaccgccccg tagcggcgag cgccgaggag
     1501 acgatcaggc aggccggcga gtcgggcttc gtgcgctttc tggcgaagag ggacggtgtg
     1561 gcgttcctga cgctcgagcc ttcgccgcag gaagaggccg ccttcgtctc gaagaagtac
     1621 ccggcggagc aggtcatgct cttctacgtc ctgcgcgaga cggcgcggct gcgcgagcgg
     1681 cggaagctcc ccgaggcgga gttgaagacg gccatcgcgg gtctccttga gaaggcttcg
     1741 aagatgaccg gcctcgctcc cttcaccacg ctcgaagggt tggaggccgc ctaccgccgc
     1801 cactggacaa cgcccgcgaa ctggtgggag gcgccgcagc agtggttcga cccgctcaaa
     1861 tcttcgcagg agacgggcgg ccttttcacc aacgacatca accgcctgtc gagcgagtac
     1921 cgcaaccggc acatgtacac ggcgctggcc tccgccgcgc tcgaaggcca gcgggtcttc
     1981 gccgtcgtcg gtcgcaacca cgtgccgatg caggcgcagg cgttgcgctg cgccgtcaag
     2041 tgacctacgc ttccgtcggt gacgcgacga caaccttcgc ctcgctcgta gtacatgaag
     2101 tcaagaagga ggcgcgaccg tgaaaaagat ttccatccac tactgcccgg tgtgaccgat
     2161 caagaaagcg ctggccgccc gtgtggcgga gcagttgaag gccgacggcg gcgtcgaggt
     2221 cgagacggtg aagggcggct tcggcgagtt cagcgtccat ctcgacggtc gcaaaatagt
     2281 cgagaccagc cggctctggt atcctacgcc gcgcaaagtc gtggagcagg tcagggcggc
     2341 gctcgccgct tgaaaaaacc ggcttcctga agtcgaaccc tcgccgcttc gaaacctcgc
     2401 cgccgtatct gcgcccccgc gcggaggagg ttccccatgt acgtgacgct cttcaacctc
     2461 gccggttacg ccatcgtcgc gtggctgctg ctcgtcttcc tgcccaagtg gagcgttacg
     2521 cgcttcgtcg cgcgcacggc catcttcccc gtctacctct cggtgctcta cgtcgtcggc
     2581 gtcgcggcgg tgctcgccga actcggcccc ggcctgatgc gcgacttcgg gagcgcggag
     2641 ggcgtcacgc gcatcctcgc gcgccaggac gtggcgctcg tcgcgtggat acacatcctc
     2701 gccttcgacc agctcgtcgc gctccacatc taccgcgaga acatggatca ccattacgtg
     2761 ccgcttcccg tccagtcggt gctcctcgtg ctgacgctga tgttcggccc cgtggggttc
     2821 ctcgcttact acgtgctgcg gctcgtctcg cgcagccgcg gcgggcggcg cgtcggcgaa
     2881 ggggaagcgg cggcggcgaa cgatgggcag cacgacccgc gccgggcgga gacggcggcg
     2941 cgcgtgagcg tgacgggccg cggcctgctg tcggtcttcg acgaagagcg cgcgctgttt
     3001 cggacggcgc tggcgggcat cgttctcggc acgctgctct tcatctacat cggcgtcaac
     3061 gggcgggcgg tgccgcccga aggcgacctc ttcaaagccg ccacgttcga catcgccgtc
     3121 ggcatctacg tgctgaccgt cacgctgctg ctccccttcg caggcttctc gccgcgcggg
     3181 cgcgcggtct ggcgctggct gaccgtcgcg ttcatgagtt acggctacgc gctggagacg
     3241 gtgcagatgc tgcgcggcct cgacccgcgc ttcacgcgcg cgggcggccc cgcggatcag
     3301 cttctcggcg gcgtcttctt cctcgtcgcg ctcggactcg ccgtgctctt cgtcgtgctc
     3361 gcgctgcgct tcttctcttc gcggacggcg gtagtcggga cgctcttcct gctcggcatg
     3421 cgctacgcgt gcgcggcggc gctgttcgcg cacggcgtcg gcctgtggat gagcctgaac
     3481 caggggccgc gcatcggcgc tgggggcaac ctgttgccgc tgcacgccgc aggcttccac
     3541 gggttgcagg ccgtgccgct cgtggcgctg cttttcgcgt ggtcgcagac gccgaccgag
     3601 gcggcgcgcc gctgggtgca cgccgccgga ctcgcgtggc tcggcgcgtg tcttgcgatt
     3661 gcgtggcaga ccgcacgcgg cggcgcggtg ctcgaacctt cctcgccgac cgcgcttgcc
     3721 gctgcgctct tcgccgtctg ggcggtcgcc ttcgcgcgcg ccgccttcgc acggcgccgc
     3781 gccggaatcg aagactattc acagcgcgcg ggcgcggcgg cctgaacgcc ggacacgaaa
     3841 gcgacgcccg gaaaacaagt ctcgccacaa agtcacgaag acacaaagag aaggcacgaa
     3901 gaagagacgg aaggtaaagc gacgaaagat gaaaagcacg ctgcttctca tcgacattct
     3961 cttcatcgtc gctccgctca tcgacgtttt tctcgtcgtc gtctcacgtt cttctcttca
     4021 tcttctctaa gtgtcttcgt ctccttgtgg atgatttttt tccacatgtg gataagtttc
     4081 atcctcccgt tcgccactct tttgagcctt caaatcaagg cgaataagga gaaaatgctt
     4141 tccaaaaaaa gtgctt
//
LOCUS       DBNGOH010000840         3436 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2692132, whole genome shotgun sequence.
ACCESSION   DBNGOH010000840 DBNGOH010000000
VERSION     DBNGOH010000840.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3436)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3436)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3436
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2692132"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2176)
                     /locus_tag="AC159P_16250"
     CDS             complement(<1..2176)
                     /locus_tag="AC159P_16250"
                     /inference="COORDINATES: protein motif:HMM:NF013324.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HIR3697046.1"
                     /translation="MNDTIFELAPYVAVGGILFIVLVVLLSSVLVNVGAKEIAIKERR
                     YLGAKMPPGRVVATEGEVGIQADVLKPGLHFIKWPFERVVRKVPLIEVGADELGVVEA
                     IDGEPMLAGSIFAPDRAQNAHNNFQDPIAFIKRGGVKGIQLRTLPPGLWPIHPYLFRV
                     SIAKTTVIPPGKVGIATSADGAPLDAGRLHGKAVEGHRNYQDAEAFIGNGGQKGPQVE
                     ILTPGTYRILTQSIPLEGGDPKPGLFFIKYYDATVIDENGIGLVEALDGAPLDPRDYV
                     AMQITGHDNFQDCNEFISRGGQRGPQKDILLPGTYYINPLVFKVIPESAKEVKPGEVA
                     VVVSNVGKDPGEEIRRAMANKVRERLEREEQEQVTKAAERLDKLDGEERRLAEIQEEL
                     LSGDPADQRLDQGAHEAYVVPEGYRGIQETVVGPGRYYVNTLAVTPIIIPTTNMTVEW
                     TAEEVDNTFDPFEVISKDGFTMKLEVRVVFRVKPEDAPFMVAKIGNTDRLIQNVMHPL
                     IDSIFRNQASESSAMAYLQNRHEEQERAEARVRMHLLKYHVDVVNVLICHIHLPEELM
                     KTQTEKILAEQRQNMYNAQREAEEKRIQLERTKSQADNQKDLMAATVGVEIAGKRAEQ
                     RKSEGEGEAHYISQTGKAEAEKVRLMGEAQGVAYSEQVNALGAQGVALVEVLKVIGEK
                     GVRITPDIMASGGSNDAGAGGGIGTLLLLNLFRNQMNLPATNG"
     gene            complement(2214..2912)
                     /locus_tag="AC159P_16255"
     CDS             complement(2214..2912)
                     /locus_tag="AC159P_16255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697047.1"
                     /translation="MSAVLIISIFLLALAAFAILRSKRSTSTDGAAPLPPMSPRALFE
                     VAETETEVRADADETRRVLTERVLRGDAKALREAHETGDRALYNDVLDALVSRFRSAF
                     PEAVQLIAVDIIRGGKLRASKSLARALLDVWDAAPTRDTTVDLLRIAALSDEAETFRE
                     TVEAVYRRWRAGELTSMKSEELSALFESEYWVLSSDARRSGAGFVLKQTLAEIGRALA
                     HEAQRESETSSEAV"
BASE COUNT          555 a         1050 c         1143 g          688 t
ORIGIN      
        1 caccatttgt tgcaggtaaa ttcatctggt tacgaaacag attaagcagc agtaaggtcc
       61 cgattccacc tccagcacca gcatcgttcg atccgcctga tgccataatg tctggtgtga
      121 tgcgtacacc cttctcgccg atcactttga gtacttcaac caacgccacg ccttgagcgc
      181 cgagtgcatt tacctgttca ctgtaagcaa caccctgcgc ttcacccatc agccgaacct
      241 tttcagcttc agccttacct gtctgcgaga tgtagtgggc ctctccctcg ccttcggact
      301 ttcgttgctc cgcgcgttta ccagcgattt cgacgccgac ggtggcggcc atcaagtcct
      361 tttggttgtc ggcctgcgac tttgtcctct ccagctgaat gcgtttttct tcagcttcgc
      421 gttgcgcgtt atacatgttc tggcgctgct cggcgagaat cttctcggtc tgcgtcttca
      481 tcagctcttc cggcagatga atgtggcaga tgaggacgtt gaccacatcg acgtgatact
      541 tcaagaggtg catgcggacg cgggcctcgg cgcgctcctg ctcttcgtgg cggttctgta
      601 gatacgccat cgcggacgac tcggacgcct gattgcggaa gatcgagtcg atcagcgggt
      661 gcatgacgtt ctggatgagg cggtcggtgt tgcctatctt ggcgaccatg aagggcgcgt
      721 cctcgggctt gacgcggaag acgacgcgca cttcgagctt catcgtgaag ccgtctttgg
      781 agatgacctc gaacgggtcg aaggtgttat cgacttcttc cgccgtccac tcgacggtca
      841 tgttcgtcgt ggggatgatg atcggcgtca ccgccagcgt gttgacatag tagcgaccgg
      901 ggccgaccac ggtctcctgt atgccgcggt agccttcggg gacgacgtag gcttcgtgcg
      961 cgccctgatc cagacgctgg tcggcggggt cgcccgagag cagctcttcc tgtatctcgg
     1021 cgaggcggcg ctcttcgccg tcgagcttgt ccaaacgctc ggcggccttc gtcacctgct
     1081 cctgctcttc gcgctcgagg cgctcgcgca ccttgttggc catcgcgcgg cgaatctcct
     1141 cgccggggtc tttgccgacg ttcgagacga cgacggcgac ctcgcccggc ttcacctctt
     1201 tggccgactc ggggatgacc ttgaagacga gcgggttgat gtagtaggtg ccgggcagga
     1261 ggatgtcctt ctgcgggccg cgctggccgc cgcggctgat gaactcgttg cagtcctgaa
     1321 agttgtcgtg gcccgtgatc tgcatcgcca cgtagtcgcg cgggtcgagc ggcgcgccgt
     1381 ccaaagcttc gaccagaccg atgccgttct cgtcgatgac ggtcgcgtcg tagtacttga
     1441 tgaagaagag gccgggcttc gggtcgccgc cttcgagcgg tatgctctgc gtcaggatgc
     1501 ggtaggtgcc gggcgtcagg atttcgacct gcgggccttt ctgcccgccg ttgccgatga
     1561 aggcttcggc gtcctgatag ttgcggtgcc cttcgacggc cttgccgtgc aggcgaccgg
     1621 cgtcgagcgg cgcgccgtcc gcggaggtgg cgatgccgac cttgcccggc gggatgacgg
     1681 tcgtcttcgc aatcgagacg cggaacaggt aagggtggat cggccacagg cccggcggga
     1741 gcgtgcggag ttggatgccc ttgacgccgc cgcgcttgat gaaggcgatg ggatcctgga
     1801 agttgttgtg cgcgttctgc gcgcggtcgg gggcgaagat gctcccggcg agcatcggct
     1861 cgccgtcgat ggcctcgacc acgcccagct cgtccgcgcc gacctcgatc agcggcacct
     1921 tgcggacgac gcgctcgaac ggccacttga tgaagtggag gccgggcttg agcacgtcgg
     1981 cctggatgcc gacctcgcct tcggtggcga cgacgcgacc gggcggcatc ttcgcgccga
     2041 ggtagcgccg ctctttgatc gcgatctcct tcgcgccgac gttgacgagc acgctgctca
     2101 gcaggacgac gaggacgatg aagagaatgc cgccgacggc gacgtaaggc gccagctcga
     2161 agatggtatc gttcatggtg ctcctcgaga gaagccgcgc ggggcggtgc cccctagacg
     2221 gcttcggagg atgtctcgga ctcgcgctgc gcctcatgcg cgagcgcgcg tcctatctcg
     2281 gcgagggtct gtttgagcac gaagcccgcc ccagaacgcc gcgcgtccga agacagaacc
     2341 cagtactcgc tctcgaacag cgcgctcaac tcttcggact tcatggacgt gagttcgccc
     2401 gcgcgccagc ggcggtagac ggcctcgacc gtctcgcgga aagtctctgc ctcgtcggag
     2461 agcgcggcga tgcgcaggag gtcaaccgtc gtgtcgcgcg tcggtgccgc gtcccagaca
     2521 tcgagcaacg cacgcgccag cgatttgctc gcgcggagtt taccgccgcg gatgatgtca
     2581 acggcgataa gctgcacggc ttcggggaag gcgctccgaa accggctcac gagcgcgtcc
     2641 agcacgtcgt tgtagagtgc cctgtcgccg gtctcatgcg cctcgcgcag cgccttcgcg
     2701 tcgccgcgca agacgcgttc ggtgagcaca cgccgcgtct cgtcggcgtc ggcgcgcacc
     2761 tctgtctctg tctctgctac ttcgaaaagt gcgcggggcg acatcggcgg gagcggcgcg
     2821 gcgccgtccg tagacgtgga gcgtttcgac cggaggattg cgaaggctgc gagggcgagc
     2881 aggaagatcg agatgataag gacggcgctc actttatcaa cgccttcagc gcgggcggcg
     2941 cgtggctgtt agttggcggc ctgccgctcc tttgcgctgt cagagaaagt tatacgcaag
     3001 tccgtggaga aagttctcgc gaggcgccga ggtttcgccg ggcgggaaca cgccggcacg
     3061 agtctgcgag cgacgaatca aacaacgccc cgccgcccgc gtcgcgcctt caaaggagag
     3121 tgggcagtga atggtggata gcgggagaca ggagtcaggg gaaggaagtc ggtagtcggc
     3181 aggcggaaga agagagaggc ttgagacgaa tcgtctcaag cctctcttgc ccttccgtct
     3241 tcaactgctc gctgcttacc gctcactcgg tcgcagcggc aagcggcgcg ggcacgaacg
     3301 cgagggcggg ggatgcgacg tggatttgtc tgccgggccg cgcggtcggg gcgacgggcg
     3361 cggcggggcc gatcttctcg gacgacttga ccttgagcat tttgaaaaaa tgctcgacgg
     3421 gtttcatttc gacgcc
//
LOCUS       DBNGOH010000841         6587 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_675035, whole genome shotgun sequence.
ACCESSION   DBNGOH010000841 DBNGOH010000000
VERSION     DBNGOH010000841.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6587)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6587)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6587
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_675035"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            9..743
                     /locus_tag="AC159P_16260"
     CDS             9..743
                     /locus_tag="AC159P_16260"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011421708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3697048.1"
                     /translation="MLAAAKAIDAPEAVFEARGLSKDYPMGEVEVHALRSVDLDLYRG
                     ELVVILGASGSGKSTLLNILGGLDTPSSGEVHYRDHDLTAAGDRALTRFRRDHVGFVF
                     QFYNLIPSLTALENVELVTEIAADPMPAAEALELVGLSDRMNHFPSQLSGGEQQRVAI
                     ARAVAKRPEVLLCDEPTGALDYKTGKLVLEVIEKVNRELGTTTAVITHNASIAEMADR
                     VLRMQSGRIVEVRRNETKKAPAELAW"
     gene            777..3140
                     /locus_tag="AC159P_16265"
     CDS             777..3140
                     /locus_tag="AC159P_16265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012167727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3697049.1"
                     /translation="MSTLDRKLFRDLLRMRGQVVAIALVVACGVAAFVAMRGTYRSLL
                     ATQSAYYAEYRFADVFAHLKRAPESLAARVGEIPGVGVAETRVVAEVTLDVPGVEEPA
                     SGRLVSIPVRTAPAVNALHLQSGRLPEPGRGEEVVISGAFSRANNLRPGDTLEAVVNG
                     RWQRLQIVGVALSPEFVYEIRGGDMFPDSRRFGVIWMSRGALGPAFDMEGAFNDLTVT
                     LAPGGSETEVIERLDRLLGQYGGTGAYGRADQASHHFISNEIAELRVTGTFIPAVFLG
                     VTAFLIHLVLSRLVSTEREQIGLLKAFGYTSFAVGFHYLKLALAAVAGGAALGVGVGW
                     WFGYRVTSLYAEFFRFPVLRYETDWQVVLAAALVSFGAATLGALAAVRRAVLLPPAEA
                     MRPEQPARFRPGLVERAGFQRLIPITARMIVRNLERYPLKALMSAFGVSLSVALLLVG
                     FYFFDAIERVIDVQFRIARREDAAVTFDEPRPARTRYELERLPGVMRVETFRAVPARL
                     RFGHHARRVGLLGVEPGGELFRIVDQDFNVHVPPPEGLLLTKELAEVLGVRPGESLTV
                     EVLEGARPVREVMVAGTVDELLGLSAYMDRRALNRLMREGGTISGARLMVDEREAARL
                     YSTLKRTPAVSGVVVPGAVLRNFNDTIARTVGTSTAVMIFFACVITVGVVYNGARIAL
                     SERGRELASLRVLGFTQREVGVMLLGEQALLTCAAVPLGYATGLCLVALITRAVDTEL
                     MRLPLVISAETFALAFVITACAALLSGLLVGWRLRRLDLIAVLKTRE"
     gene            3145..4080
                     /locus_tag="AC159P_16270"
     CDS             3145..4080
                     /locus_tag="AC159P_16270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017325590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR3697050.1"
                     /translation="MNGRRRKLRLAPTVAAVLALAGITAVPVLRPKPLRVETACAVRG
                     PLVVTIDAEGRTRVRERFTVAAPVTGLLRRIPLHSGDPVYAGATVAVIEPAPLSSVSM
                     PGQGGSYVPQSAIVRAPMNGRVLRVLEQSERVVTTGTPLLELSNTTQLELVVDVLSTD
                     AVKVSPGASVLVENWGGDEPLRGRVRLIEPSAFTKVSALGVEEQRVNVVADFNDSTGR
                     VGDGFRVEARVVVWEGETLKVPSGAFFRHGGAWGVFVVEGGKVRRVEVEAGRRTSSEV
                     EIRSGLNEGDMVVLHPSNELEDGAKVEPPASSNDD"
     gene            4100..4438
                     /locus_tag="AC159P_16275"
     CDS             4100..4438
                     /locus_tag="AC159P_16275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697051.1"
                     /translation="MSSTTFNVMLLLPSFALVFAVLASLYGARRAYVTLSWVPVALLV
                     MVYALQQISKFQPYPIYGPDVRMAVGWAALVQCLFGVTLAARAVWRGQSCTGLMLATC
                     LAGVPYLLRD"
     gene            4468..4971
                     /locus_tag="AC159P_16280"
     CDS             4468..4971
                     /locus_tag="AC159P_16280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697052.1"
                     /translation="MRRMLSAVVLLFVMSLAGGAVAEYVRPKMAVAPTSQKAAGAAED
                     FLLLHDGAHVRVRFREQAGPASIRFLSLSDSSRLDIINPLVGAEKYVFRKEQGWQTYP
                     HAVAGQVVTVILRETGAGAVSEWDLPGCSDVIVRIDNAAIFAPDGAEVGAPLGARCPV
                     CGAVLDW"
     gene            5030..>6587
                     /gene="ppsA"
                     /locus_tag="AC159P_16285"
     CDS             5030..>6587
                     /gene="ppsA"
                     /locus_tag="AC159P_16285"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: protein motif:HMM:NF005057.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HIR3697053.1"
                     /translation="MTGKIIYTLDFGQLTLKDVARVGGKNASLGQLFNTLRPAGVGVL
                     DGFATTADAYRRLLAENHLEYKLRSLLTDFDVEDVAELAVRGHAARAAVMETPLPEEL
                     RAAIVGAYERLCVRLGREPELAVRSSATAEDLPEASFAGQQETVLNVRGGEALLRAVH
                     MCYASLFTDRAVSYRHRHGFDHLDVALSVGVQPMVRSDKASAGVVFTLDTESGFRDAV
                     VVTAAYGLGEYVVQGVVTPDEWTVFKPTLKAGFNPVVGRRLGTKEVRLVYSDGTRPTR
                     GEPVPEAERRRFCLTDDEVLKLARWACIIEEHYSRLAGREQPMDIEWAKDGVTGELFV
                     VQARPETVHSSKSRAAAAEVYRLKGEHGRPLVKGQAVGEKVGTGKVRVVKDVSGLVRV
                     EAGDVLVAANTDPDWEPVMKRVAAVVTDRGGRTAHAAIVSRELGLPCVVGTGNATEVL
                     RDGQQVTVSCAEGTEGHVYDGRVPFEVERVDAAAVPATRTRVMLNVGDPEKAFSLAAI
                     PNAGVGLARTE"
BASE COUNT          948 a         2206 c         2342 g         1091 t
ORIGIN      
        1 aacagggaat gctcgccgcc gctaaagcca tcgacgcacc cgaggccgtc ttcgaggcgc
       61 gcggcctgtc gaaggactac ccgatgggcg aggtcgaggt gcacgcgctc cgctccgttg
      121 acctcgacct ttaccggggc gagttggtgg tgatcctcgg cgcttcgggg tcggggaagt
      181 ccacgctgct gaacatcctc ggcgggctgg acacgccttc gagcggcgag gttcattacc
      241 gcgaccacga cctgacagcg gcgggcgacc gcgcgctcac gcgcttccgc cgcgaccacg
      301 tcggcttcgt cttccagttc tacaacctca tcccctcgtt gacggcgctg gagaacgtcg
      361 aactcgtcac cgagatcgcc gcagacccga tgcccgcggc ggaggcgctt gagctggtcg
      421 gactctccga ccgcatgaac cacttcccct cgcagctctc gggcggcgag cagcagcgtg
      481 tcgccatcgc gcgcgccgtc gccaagcgac cagaggtgct gctgtgcgac gagccgacgg
      541 gcgcgctcga ctacaaaacc ggcaagctcg tgctcgaagt catcgagaag gtgaacaggg
      601 aactcggcac gacgacggcg gtgattacgc acaacgcctc catcgccgag atggccgacc
      661 gcgtgctgcg gatgcagagc gggcggatcg tcgaagtccg ccgcaacgag acgaagaaag
      721 cacccgccga gctggcgtgg tgacgaacct cgcgtcgaag cagacggatt gtttttatgt
      781 ccacgctcga ccgcaaactc ttccgcgacc tcctgcgcat gcgcgggcag gtcgtcgcca
      841 tcgcgctcgt cgtcgcctgc ggcgtcgccg cgttcgtggc gatgcgtggc acctaccgct
      901 cgctcctcgc cacgcagtcg gcctactacg ccgagtaccg cttcgccgac gtgttcgcgc
      961 acctgaagcg cgcgcccgaa tcgttggctg cgcgcgtcgg tgagattccc ggcgtcggcg
     1021 tggccgagac gcgcgtcgtg gccgaggtta cgctcgacgt gcccggcgtc gaggagcctg
     1081 cttcgggtcg cctcgtttcg attcccgtcc gcaccgcgcc cgctgtcaac gccctgcact
     1141 tacaaagcgg gcgattgccc gagccgggcc gcggcgagga ggtcgtcatc agcggcgcct
     1201 tctcccgcgc caataacttg cggcccggcg acacgctcga ggccgtcgtc aacgggcgct
     1261 ggcagcgctt gcagattgtc ggcgtcgcgc tctcgcccga gttcgtctac gagatacgcg
     1321 gcggcgacat gttccccgac agccgccgct tcggcgtcat ctggatgagc cgcggggcgc
     1381 tcggccccgc cttcgacatg gaaggcgcgt tcaacgacct caccgtcacg ctcgcgcccg
     1441 gaggcagcga gacggaagtc atcgaaaggc tggatcggct gctcgggcag tacggcggca
     1501 cgggcgctta cgggcgcgcg gatcaggcgt cgcaccactt catctcgaac gagatcgccg
     1561 agctgcgcgt gacgggcacc ttcatccccg cggtcttcct cggcgtgacg gccttcctca
     1621 tccacctcgt cctgtcgcgc ctcgtcagca cggagcgcga acagatcgga ctgctgaagg
     1681 cgttcggcta cacgagcttc gccgtcggct tccactacct caagctggcg ctcgcggcgg
     1741 tcgcgggcgg cgcggccctc ggcgtgggcg tcggctggtg gttcggctac cgcgtcacgt
     1801 cgctctatgc ggagttcttc cgcttccccg tgctgcgtta cgagaccgac tggcaggtcg
     1861 tccttgcggc ggcgctcgtc agcttcggcg cggccacgct cggggcgctc gcggcggtgc
     1921 ggcgggccgt gctgctcccg cctgccgagg cgatgcggcc cgagcagccc gcgcgcttcc
     1981 gccccggact cgtcgagcgc gcgggcttcc agcgactcat ccccatcacc gcgcggatga
     2041 tcgtacgcaa cctcgaacgc tacccgctga aggccctgat gagtgccttc ggcgtctcgc
     2101 tctcggtcgc gctcctcctc gtcggtttct acttcttcga cgccatcgag cgcgtcatcg
     2161 acgtgcagtt ccgcatcgcc cgccgcgagg acgccgccgt taccttcgac gagccgcgcc
     2221 ccgcgcgcac gcgttacgag cttgagcggc tgcccggcgt gatgcgcgtc gagacgttcc
     2281 gcgccgtgcc cgcgcgtctg cgcttcggcc accacgcgcg gcgcgtcggg ctgctcggcg
     2341 tcgagccggg cggcgagctg ttccgcatcg tcgatcagga cttcaacgtc cacgtgccgc
     2401 cgcccgaagg tctgttgttg acgaaagagc tggcggaggt gctcggcgtg cggcccggcg
     2461 agagcctgac ggtcgaggtg ctcgaagggg cgcggcccgt ccgcgaggtc atggttgccg
     2521 ggacggtgga cgagttgctc ggcctctccg cttacatgga caggcgcgct ctgaatcgcc
     2581 tgatgcgcga gggcgggacg atctcgggcg cgcgcttgat ggtggacgag cgcgaggccg
     2641 cgcgcctcta ctcgacgctc aaacgcaccc cggctgtgag cggcgtcgtc gtgcccggcg
     2701 ccgtactgcg gaacttcaac gacaccatcg cgcgcacggt cggcacttcg acggcggtga
     2761 tgatcttctt cgcctgcgtc atcacggtcg gggtggtcta caacggcgcg cgcatcgcgc
     2821 tctcggagcg cgggcgcgag ctggcttctt tgcgcgtgct cggcttcacg cagcgcgagg
     2881 tcggcgtgat gctgctcggc gagcaggctt tgctgacgtg cgcggcggtg ccgctcggct
     2941 acgcgacggg cctctgcctc gtcgcgctga ttacgcgcgc ggtggacacg gagctgatgc
     3001 gcctgccgct cgtgataagc gccgagacgt tcgcgctggc gttcgtcatc accgcgtgcg
     3061 ccgcgctcct ttccgggttg ttggtcgggt ggcgactgcg ccggctcgac ctcatcgccg
     3121 tgctgaagac gagggagtga agccatgaac gggagacgca ggaaactcag gctcgcccca
     3181 acggtcgccg cggtgctcgc gctcgccggc ataacggctg ttcccgtctt gcgcccgaag
     3241 cctttgcggg tggagacggc ctgcgccgtg cgcggcccgc tcgtggtgac gattgacgcc
     3301 gaagggcgga cgcgcgtgcg cgaacgcttc acggtggccg cgccggtgac gggcctcttg
     3361 agacgcatcc ccttgcacag cggcgacccg gtgtacgcgg gcgcgacggt ggccgtcatc
     3421 gaacccgcgc ccctgtcatc cgtgtcgatg cccggtcagg gcggctctta cgtcccgcag
     3481 tcggccatcg tccgtgcgcc catgaacggg cgggtgctgc gcgtgctcga acagagcgag
     3541 cgcgtggtca cgacggggac gccgctcctt gagctgagca acaccacgca gcttgagctg
     3601 gtggttgacg tgctctcgac ggacgccgtg aaggtctcgc ccggcgcgtc cgtcctcgtc
     3661 gagaactggg gcggtgacga accgctccgg gggcgcgtcc gcctgataga gccttcggct
     3721 ttcacgaagg tctccgcgct gggcgtcgag gagcagcgcg tcaacgtcgt cgccgacttc
     3781 aacgactcga cgggccgtgt cggcgacggc ttccgcgtcg aagcgcgcgt cgtcgtctgg
     3841 gagggcgaga cgctgaaggt gccttcgggc gcgttcttcc gccacggcgg ggcttggggc
     3901 gtgttcgtcg tcgagggagg gaaggtgcga cgtgtcgagg tcgaggcagg gcgccgcacc
     3961 tcctccgagg tcgagatcag gtcgggcctg aacgaaggcg acatggtcgt cctccacccc
     4021 tcgaacgagt tggaggatgg cgcaaaggtc gagccgccgg cgagttcgaa cgacgactga
     4081 gcgatgaagg gagtgggaaa tgtcttcgac gactttcaac gtgatgctcc tgttgccgtc
     4141 gttcgccctc gtcttcgcgg tgctggcgtc gctctacggc gcgcgtcgcg cgtacgtcac
     4201 gctctcgtgg gtgcccgtcg cgctgctcgt gatggtctac gcgctccagc aaatatcgaa
     4261 gtttcagccg tacccgatct acgggccgga tgtccgcatg gccgtcgggt gggccgctct
     4321 cgtccagtgc ctcttcggcg tgacgttggc ggcgcgggcc gtgtggcgag gacagagttg
     4381 tacggggctg atgctcgcga cgtgtctggc gggcgtcccg tacctgctcc gcgattgaat
     4441 cgaccgcgcg gagaggaggt gaacgagatg agaagaatgt tgtcggcagt ggttctgctg
     4501 tttgtcatgt cgctggcggg aggagcggtc gccgagtacg tcaggccgaa gatggccgtc
     4561 gcaccgacct cacagaaggc cgcgggcgcg gcggaagact tcctgctgct gcacgacggt
     4621 gctcacgtca gggtgcggtt ccgcgagcag gcggggccgg cgagcatccg tttcctgagc
     4681 ctcagcgaca gctcgcggct cgacatcatc aacccgctgg tcggcgccga gaagtacgtc
     4741 tttcgtaagg agcagggctg gcagacctac ccgcacgcgg tcgccgggca ggtcgtcacc
     4801 gtcatcctcc gcgagacggg cgccggcgca gtttccgagt gggatttgcc cggctgctcg
     4861 gacgtgatcg tgaggataga taacgcggcc atcttcgcgc cggacggcgc ggaggtcggc
     4921 gcgcctctcg gcgcgaggtg tccggtctgc ggcgcggtgc tcgactggtg acgagagtcg
     4981 tcggcagaag gtcaagccct cgcgtcggat gaaagtgagg tgaacggcaa tgaccggcaa
     5041 gataatttac acgctcgatt tcggacagct cacgttgaag gacgtggcgc gggtcggagg
     5101 caagaacgcc tcgctcggcc aactcttcaa cacgctccgg cccgcgggcg tcggcgtcct
     5161 cgacggcttc gcgacgaccg ccgacgccta ccgcagactt ctcgccgaga accatctgga
     5221 atacaagctg cgctcgctgc tcacggattt cgacgtggag gacgtggcgg aactcgccgt
     5281 cagggggcac gcggcacgcg ccgccgtcat ggagacgccg ctgccggaag agctgcgcgc
     5341 cgcaatcgtc ggcgcttacg agcggctgtg cgtgcggctc gggcgcgagc ctgagctggc
     5401 cgtgcgctcg tcggccacgg cggaggactt gcccgaagcg tctttcgcgg gccagcagga
     5461 gaccgtcctc aacgtccgcg gcggcgaggc gctgctgcgc gccgtgcaca tgtgctacgc
     5521 ctcgctcttc accgaccgcg ccgtcagcta ccgccaccgc cacgggttcg accatctgga
     5581 cgtggcgctc tcggtcggcg tgcagccgat ggtgcgctcg gacaaggcga gcgccggcgt
     5641 cgtcttcacg ctcgacaccg agtcggggtt ccgcgacgcg gtcgtcgtga ccgccgcata
     5701 cggcctcggc gagtacgtcg tgcagggcgt ggtcacgccc gacgagtgga cggtcttcaa
     5761 gccgacgctc aaggcaggct tcaaccccgt cgtcgggcgg cggctcggca cgaaggaggt
     5821 gcggctggtc tattcggacg gcacgcgccc gacgcgcggc gagccggtgc cggaagcaga
     5881 gcgccgccgc ttctgtctga cggacgatga ggtgctcaag ctcgcgcgat gggcgtgcat
     5941 catcgaggag cactattccc ggctcgccgg gcgcgaacag ccgatggaca tcgaatgggc
     6001 gaaggacggc gtgacgggcg aactcttcgt cgtgcaggcg cggcccgaga cggtgcattc
     6061 gtcgaagtcg cgggcggcgg cggcagaggt ttacaggctg aagggcgaac acggcaggcc
     6121 gctcgtcaag ggtcaggccg tgggcgagaa ggtcggcacg gggaaggtga gggtggtgaa
     6181 agacgtgtcg ggcctcgtac gcgtcgaggc gggcgacgtg ctcgtcgccg cgaatacaga
     6241 cccggactgg gagccggtga tgaagcgagt ggccgcggtc gtcaccgacc gcggcgggcg
     6301 cacggcccac gccgccatcg tctcgcgcga gctggggctg ccgtgcgtcg tcggcacggg
     6361 caacgcgacc gaggtcttgc gcgacgggca gcaggtgacg gtctcttgcg ccgaaggcac
     6421 cgaaggccac gtctacgacg ggcgcgtgcc gttcgaggtc gagcgcgttg acgccgccgc
     6481 cgtgcccgcg acgcgcaccc gcgtgatgct caacgtcggc gacccggaga aagccttctc
     6541 gctcgccgcg atcccgaacg cgggcgtcgg cctcgcgcgc accgagt
//
LOCUS       DBNGOH010000842         3467 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2141366, whole genome shotgun sequence.
ACCESSION   DBNGOH010000842 DBNGOH010000000
VERSION     DBNGOH010000842.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3467)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3467)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3467
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2141366"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..339
                     /locus_tag="AC159P_16290"
     CDS             <1..339
                     /locus_tag="AC159P_16290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HIR3697054.1"
                     /translation="VKTLVMETETRKALIVLMHGDREVSTKQLARRLGTKSVQPCAPE
                     TAQKHTGYVVGGTSPFGTRARLPVYVERTIFGLPKIYINGGKRGFLVSIDPRVLRDVL
                     PVEEVEVAIQ"
     gene            427..1605
                     /locus_tag="AC159P_16295"
     CDS             427..1605
                     /locus_tag="AC159P_16295"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HIR3697055.1"
                     /translation="MRDAVIVSAVRTAVGKAPKGTLRNTRPDEMGAAVIEESIKRAAG
                     LDKAEIEDVIMGCAMPEAEQGMNVARAAAIRAGLPVETSAMTINRFCSSGLQSIALAA
                     DRIRAGGADVVVAGGLETMSMIPMGGHIIRPNPYLVEHYPDFYLNMGLATENVARKYE
                     VSREEQDAFALRSHRRAAAAQDAGKFDDEIVPLSVTFEELNAKGKKERRETVFTKDEG
                     VRRDTSEEGLAKLRPAFHAQGTITAGNASQMSDGAAVAVIMSDERARALNLKPLARFV
                     AYATAGCPPEEMGIGPVYAIPKVLKLAGLKLEDIDVIELNEAFAAQSLAVIKTLGLDP
                     ERVNVNGGAIALGHPLGCTGAKLTATILGELERRNGRYGMVTMCVGGGMGAAGIFERL
                     "
     gene            1695..>3467
                     /locus_tag="AC159P_16300"
     CDS             1695..>3467
                     /locus_tag="AC159P_16300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR3697056.1"
                     /translation="MSAVAEEKKIIVGGSFLIEERTTAEVFTPEDFTEEHRMIADTAR
                     QFMDNEVRPRLDELEKTKDWNIARELVKKGAEIGLVGATVPEEYGGLGLDQVSGAIIG
                     ELLGRAASFATTMGAQSGIGLLPILYYGTEEAKQKYLPKLVTGEMITAYALTEAGSGS
                     DALAAKATARLSEDGKTYVLKGEKMWITNGGFADVFVVFAKVDGDKFSGFIVERQEGL
                     QNGAEEHKMGIKGSSTTALALDNVKTPVENLLGEVGKGHKIAFNILNIGRFKLGAMCN
                     GGMKLMAQEAVRYANERHQFGKPISSFGAIKSKLADMAIKTWIGEAMVYRTLGMIEGA
                     INANADSTDPEAKLRAIEEYAAECSIIKVALSEYCDFVADEMEQIFGGYGYSADYPAE
                     RAYRDSRINRIFEGTNEINRMLIPGMLMKRAMTGKLALLPAAQGLMEEVLSPSLPSLD
                     DEEGVLANEARLVRNAKKVGLMVLGTAAQKYMAALSEEQEILIGAADILMDVYAMESA
                     VLRAQKLAAAQGEEAAALYLDMVRVFCNDAVERVEANAKNTLAAMAEGDELRTLLAAL
                     RRFTKNTPANTVAPRRRIADAMVKA"
BASE COUNT          692 a         1069 c         1180 g          526 t
ORIGIN      
        1 gtgaagactt tggtgatgga gaccgagacg cgcaaagccc tgatcgtcct gatgcacggc
       61 gaccgcgagg tctcgacgaa gcagttggcg cggcgcctcg gcacgaagag cgtgcagccc
      121 tgcgcccccg agacggcgca gaagcacacg ggctacgtgg tcggcggcac cagccctttc
      181 ggcacgcgcg cgcggttgcc ggtctacgtc gagcgcacga tcttcggcct gccgaagatt
      241 tacatcaacg gcggcaagcg cggcttcctc gtttcgattg acccgcgcgt gctgcgcgac
      301 gtgctcccgg tcgaagaggt cgaggtggcg attcaataag ctgttagcga ttagctgtta
      361 gctgttagct aaagacaaaa agccaatagc taaccgctaa tcgctaacag ctatcatgga
      421 gtgattatga gagacgcagt catcgtttcc gccgtgcgga cggcggtcgg caaggcgccc
      481 aaagggactt tgcggaacac gcggcccgac gagatgggcg ccgccgtcat cgaggagtcg
      541 atcaagcgcg ccgcggggtt ggacaaggcc gagatcgaag acgtgatcat gggctgcgcg
      601 atgccggagg ccgagcaggg gatgaacgtg gcgcgcgcgg cggcgatacg cgcggggctg
      661 cccgtggaga cgagcgcgat gacgatcaac cgcttctgct cctcgggcct gcaatccatc
      721 gcgctggccg ccgaccgcat tcgcgcgggc ggcgccgacg tagttgtggc gggcggactc
      781 gaaacgatgt cgatgatccc gatgggcggc cacatcatcc ggcccaaccc gtatctggtc
      841 gagcactacc cggacttcta cctcaacatg ggtctggcga ccgagaacgt cgcgcgcaag
      901 tacgaggtgt cgcgcgaaga gcaggacgcc ttcgcgctcc gctcgcaccg gcgcgccgcc
      961 gccgcgcagg acgcagggaa gttcgacgac gagatcgtgc cgctgagcgt gacctttgaa
     1021 gagttgaacg cgaagggcaa aaaggagcgg cgcgagaccg ttttcacgaa agacgagggc
     1081 gtgcgccgcg acacgagcga agagggcttg gcgaagctca ggcccgcctt ccacgcgcag
     1141 ggcacgatca cggcgggcaa cgcctcgcag atgtcggacg gcgcggctgt cgccgtcatc
     1201 atgtccgacg agcgggcgcg cgcgctcaac ctgaaaccgc tggcgcgctt cgtcgcctac
     1261 gccaccgccg ggtgcccgcc cgaagagatg ggcatcggcc ccgtctacgc catcccgaaa
     1321 gttttgaagc tcgcgggtct caagctcgaa gacatcgacg tgatcgagct gaacgaagcc
     1381 ttcgccgcgc agagcctggc cgtcatcaag acgctcggcc tcgaccccga gcgcgtcaac
     1441 gtcaacggcg gcgccatcgc gctcggccac cccttaggct gcaccggcgc gaagctgacg
     1501 gccaccatcc tcggcgaact cgaacgccgc aacggtcgct acggcatggt cacgatgtgc
     1561 gtcggcggcg gcatgggcgc cgcgggaatt ttcgaaagac tgtaagcagt aagcagtgag
     1621 ccgtgagcag tagaaataac aaactgctta ctgcttaccg ctcactgctc acttattgtt
     1681 gaaaggagca aatcatgtct gctgttgcgg aagagaaaaa gatcatcgtg ggcgggagtt
     1741 ttctgatcga agagaggacg accgcggagg tcttcacgcc cgaggatttc acggaggagc
     1801 accgcatgat cgccgacacc gcgcggcagt tcatggacaa cgaggtgcgt ccgcgtctgg
     1861 acgagctgga gaagacgaag gactggaaca tcgcgcgcga gctggtgaag aagggcgcgg
     1921 agatcggtct ggtgggcgcg accgtgccgg aggagtacgg cggcctcggc ctcgaccagg
     1981 tgagcggcgc catcatcggc gagctgttgg ggcgggcggc ctcgttcgcc acgacgatgg
     2041 gcgcgcagag cggcatcggc ctgttgccga ttctctacta cggcacagag gaggcgaagc
     2101 agaagtacct gccgaagctc gtcacgggcg agatgattac ggcctacgcg ctgaccgagg
     2161 ccgggtcggg ttcggacgcg ctcgcggcga aggcgaccgc gcgcctctcg gaagacggca
     2221 aaacttacgt cctcaagggc gagaagatgt ggatcacgaa cggcggcttc gcggacgtgt
     2281 tcgtcgtctt cgccaaggtg gacggcgaca agttcagcgg cttcatcgtc gagcggcagg
     2341 aggggcttca gaacggcgcc gaagagcaca agatgggcat caagggttct tcgacgacgg
     2401 cgctcgcgct cgacaacgtg aagacgccgg tcgagaacct cttgggcgag gtcggcaagg
     2461 ggcacaagat cgccttcaac atcctcaaca tcggtcgctt caaactcggc gcgatgtgca
     2521 acggcgggat gaagctgatg gctcaggagg ccgtgcgcta tgccaacgag cgccaccagt
     2581 tcggcaagcc catctccagt ttcggcgcca tcaagtcgaa gctcgccgac atggcgatca
     2641 agacgtggat cggcgaggcg atggtctacc gcacgctcgg catgatcgag ggcgccatca
     2701 acgccaacgc cgactcgacc gacccggaag cgaagctgcg ggccatcgag gagtacgccg
     2761 ccgagtgctc gatcatcaag gtggcgctgt cggagtactg cgacttcgtc gccgacgaga
     2821 tggagcaaat cttcggcggc tatggctact ccgccgacta cccggctgaa agggcctacc
     2881 gcgactcgcg catcaaccgc atcttcgagg gcacgaacga gatcaaccgc atgctcatcc
     2941 ccggcatgct gatgaagcgc gccatgacgg gcaagctcgc gctgctcccg gcggcgcagg
     3001 gcttgatgga agaggtgctg tcgccgagtc tcccctcgct cgacgacgaa gagggcgtgc
     3061 tggcgaacga ggcgcggctc gtccggaacg cgaagaaggt cgggctgatg gtcttgggca
     3121 cggcggcgca gaagtacatg gcggctcttt cagaggagca ggagattctg atcggcgcgg
     3181 cggacatcct gatggacgtg tacgcgatgg agtccgccgt cctgcgcgcg cagaagctcg
     3241 ccgcggcgca aggtgaagaa gctgccgcgc tctacctcga catggtgcgc gtcttctgca
     3301 acgacgccgt cgagcgcgtc gaggccaacg ccaagaacac gctcgccgcg atggccgagg
     3361 gcgacgagct gcgcacgctc ttggccgcgc tccgccgctt caccaagaac acgcccgcca
     3421 acaccgtcgc cccgcgccgc cgcatcgccg acgcgatggt caaggcg
//
LOCUS       DBNGOH010000843         2774 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3977080, whole genome shotgun sequence.
ACCESSION   DBNGOH010000843 DBNGOH010000000
VERSION     DBNGOH010000843.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2774)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2774)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2774
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3977080"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1599)
                     /gene="asnB"
                     /locus_tag="AC159P_16305"
     CDS             complement(<1..1599)
                     /gene="asnB"
                     /locus_tag="AC159P_16305"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0070981 - L-asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HIR3697057.1"
                     /translation="MCGIAGLITTEAGARVGAMLRAVAHRGHDGEGVWESGVIDAEGR
                     RACLGHRRLAIIDTSAAGLQPKLSADGRFVVTFNGEIYNYRELRRELAAKGHVFRTET
                     DTEVLLAAFAEWGEGCLPRLNGMFAFALWDERERALLLVRDRLGIKPLYYSITRGVDG
                     AAAVESLVFASEVKAILASGLVGRALDVEGLHQQLTFLWTPDPHTLFRDVWKVPPGHL
                     LKWQDGRVSLREWWDVSFDEVEEGRGEEWWRERVLETLERVVGMEMVSDVPLGSFLSG
                     GVDSSSIVAMMKRHAHGRRVSTYTVGIEQEDLRYDIIPDDTRWARRVAKALDTDYHEM
                     ILQPDVAELLPLLVYHMEMPVIDMAVSSYLVSRAARETLTVMLSGMGGDEVFAGYPRQ
                     LAMQVARALDPLPLSVRRPAMRAIAGALPGGMPGRLTAPLRNAKKFARSAALDFEERY
                     LGYGTYFTDEAKHDIYSDRLREETRGMDAYRHHRRYFARCQEAAPLNRLLYVDLKTFQ
                     PCLNLDYTDKTAMAATLEVRVPLLN"
     gene            complement(1731..2588)
                     /locus_tag="AC159P_16310"
     CDS             complement(1731..2588)
                     /locus_tag="AC159P_16310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697058.1"
                     /translation="MPDDDNSPDSSNQPLSQFGGTEQLQDTSAQLAGNTVSVSPGGAT
                     FPTIAAALASITDNNLRKQYVINVGPGTFNEQVTLKPYVYLHGAGLDQTTINTPPASG
                     NNSFGRGTVIGASNSGVYHVKLTCLGGSWGDWSTALAIPACSPFYVGDVWLVSDDQGN
                     RGVNIETCSVNWNLANQQGAALVYFSSVAILANGQSSDSVPQALIVGAQAVVQLIESK
                     VVSQGAGQSFGGTTALGGQLTLDNCFVQGGTWALYNSDGGSPITANNCQFNGPVSNGV
                     TVNNFPPPQ"
BASE COUNT          434 a          950 c          937 g          453 t
ORIGIN      
        1 gttcagcagc ggcacgcgca cttccaacgt cgccgccatc gccgtcttgt ccgtgtagtc
       61 gaggttcaga cacggctgga aagtcttcag gtcaacgtac aggagccggt tgagcggcgc
      121 ggcctcctgg cagcgcgcga agtagcggcg gtggtgccgg taggcgtcca tgccgcgcgt
      181 ctcttcgcgc aggcggtcgc tgtagatgtc gtgcttcgcc tcgtccgtga agtaggtgcc
      241 gtagccgagg tagcgctcct cgaagtcgag cgcggcgctg cgcgcgaact tcttcgcgtt
      301 gcgcaagggc gccgtcagac ggccaggcat cccgccgggg agcgcgcccg caatcgcgcg
      361 catggcgggc cgccgcacgc tcaaaggcaa ggggtcgagc gcccgcgcca cctgcatcgc
      421 caactgccgc ggatagcccg cgaagacctc gtcgccgccc atccccgaca gcatcaccgt
      481 cagcgtctcg cgcgcagcgc gcgacaccag atagctcgac accgccatgt cgatgaccgg
      541 catctccatg tggtagacga gcagcggcaa cagctcggcc acgtccggct gcaagatcat
      601 ctcgtggtag tcggtgtcga gcgccttggc gacgcgtcgc gcccagcgcg tgtcgtccgg
      661 gatgatgtcg tagcgcaagt cctcctgctc gatgccgacc gtgtaggtcg agacccggcg
      721 cccgtgcgcg tggcgcttca tcatagcgac gatcgacgag gaatcgacgc cgcccgacag
      781 gaaagacccg agcggcacgt ccgataccat ctccatcccg acgacgcgct ccagcgtttc
      841 gagcacgcgc tcgcgccacc actcctcgcc ccgcccctct tcgacttcgt cgaacgacac
      901 gtcccaccac tcgcgcaggc tgacgcgccc gtcctgccat ttcaggaggt ggcccggcgg
      961 caccttccac acgtcgcgga agagcgtgtg cgggtcgggc gtccacagga acgtgagctg
     1021 ctgatggaga ccttcgacat cgagcgcgcg cccgaccagc cccgacgcga gaatcgcctt
     1081 cacctccgac gcgaagacca gcgactcgac cgccgccgcg ccgtcaacgc cgcgcgtgat
     1141 tgagtagtac aacggcttga tgccgagccg gtcgcgcacg agcaggagcg cacgctcccg
     1201 ctcgtcccac aaggcgaacg cgaacatccc gttcagacgc ggcaggcacc cctcgcccca
     1261 ctcggcgaac gccgcgagca gaacttccgt gtccgtctcc gtgcggaaga cgtggccctt
     1321 cgctgcaagc tcgcgccgca gctcgcggta gttgtaaatc tcgccgttga aggtgacgac
     1381 gaagcgcccg tccgccgaca gcttgggctg caaccccgcc gcgctcgtgt cgatgatcgc
     1441 cagccgcctg tgccccagac acgcgcgccg cccctccgca tcgatcacgc ccgactccca
     1501 cacgccctcc ccgtcgtgcc cgcggtgcgc gacggcacgc agcatcgcgc ccacgcgcgc
     1561 gcccgcttcg gtcgtgatga gtccggctat tccacacata cggatgcgtc gggatgcggc
     1621 ccgaatcgta aatcacgcgg cggcgttcgc ccaagcggcg ccgccggcga caaagaaagg
     1681 gcgagcggct gaaggctcgc ccttgcattt cgcggtgccg ctcggcccga ctactgcggc
     1741 ggcggaaagt tgttgaccgt cacgccgttc gacaccgggc cgttgaactg gcagttgttg
     1801 gccgtgatgg gcgagccgcc gtcggagttg tagagcgccc aggtgccgcc ctgcacgaaa
     1861 cagttgtcga gcgtcagttg gccgccgagc gcggtcgtgc cgccgaacga ctgccccgcg
     1921 ccctgggaga cgaccttcga ctcgatgagc tggacgacgg cctgcgcccc gacgatgagc
     1981 gcctgcggca ccgaatcgct gctttgcccg ttggcgagga tggccacgga ggagaagtag
     2041 acgagcgccg cgccctgctg gttggcgagg ttccagttga cggagcacgt ctcgatgttg
     2101 acgccccggt tgccctggtc gtcggagacg agccacacgt cgccgacgta gaaggggctg
     2161 cacgcgggga tggcgagcgc cgtgctccag tcgccccagc tcccgccgag gcaggtcagc
     2221 ttcacgtggt agacgcccga attgctcgcg ccgatgaccg tgccgcgccc gaacgagtta
     2281 ttgccggagg cgggcggggt gttgatggtc gtctgatcca gccccgcgcc gtgcaggtag
     2341 acgtaaggct tgagcgtcac ctgctcgttg aacgtgccgg ggccgacgtt gatgacgtac
     2401 tgcttccgga ggttgttgtc ggtgatgctg gcgagcgcgg cggcgatggt cgggaaggtc
     2461 gcgccgccgg ggctgacgga gacggtgttg ccggccaact gcgcggaggt gtcctggagc
     2521 tgctccgttc cgccgaactg ggagaggggt tggttgctgc tgtcgggcga gttgtcgtcg
     2581 tcaggcattt tctcttctcc ttaaggggtg gatttttttt ccgtgaaagc ggcgagaggt
     2641 ctcggagctt cgaagaatcc gccgacccgg cggggcgggg gcgttgcggc tccgagtcac
     2701 gcggcggcct tcggcgtctg tctggggaaa cgtggaatcg tcctgcgcgg tgattctagc
     2761 agacgcgcgc gccc
//
LOCUS       DBNGOH010000844         7518 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_310756, whole genome shotgun sequence.
ACCESSION   DBNGOH010000844 DBNGOH010000000
VERSION     DBNGOH010000844.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7518)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7518)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7518
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_310756"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..844)
                     /locus_tag="AC159P_16315"
     CDS             complement(<1..844)
                     /locus_tag="AC159P_16315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697059.1"
                     /translation="MTTSISNASAGSRSHADAAPSAELLAPPPAPPPPYAPSAESAAP
                     RTIWSRFIILLLCLAVVTTTLAFGTVHAWSLAAFQLSAGVVVALWMIDAWRTRSLRFS
                     KSFLQVPLLGLALLGLVQLLPLGAGGAENIGAAGLSVEPARALSFDPYATRLVLIQLS
                     ALFVYFAAALAFIDSPRRLRLVVRTVIIFGFVLALFGLMQHFLNPYVIYWVREPKQAQ
                     PFGPYINRHHFAGYMELALAMPLGLLFSGAIDRDRIPLYAFAALLCSVALVMTNSRGG
                     IIAMV"
     gene            complement(795..2096)
                     /locus_tag="AC159P_16320"
     CDS             complement(795..2096)
                     /locus_tag="AC159P_16320"
                     /inference="COORDINATES: protein motif:HMM:NF025910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3697060.1"
                     /translation="MSDQIAFINVRPLLLRVPLMLVAVLALFAAWCGVRWLVGNTMAE
                     SARDFETGESAERLAPDDPYARLQLARLRRVSLLPEELPEALRQYERAAALAPNDYLI
                     WMELGRARASAGDAEGGIRALRRATELAPSYAQPRWHLGNVLLRAGQVDEAFAELRRA
                     ADADAGLRPQVFNLAWQVYDRDVKRVVASIGDSPTARAQLAMVLVGRGLLDEALALWA
                     GLSAEEKRANRGGAGEMLVKALFGKKLYRAASQVLREAGDEGAAEGAVGNGGFEADIA
                     PAGKKYFGWQVVPPEGVQVAVDPRVKHGGARSLRLSFNVPMHVDFKGVLQLVTVEPAT
                     RYRLGFVTRAEDLKSASTPVVEVLDGASGAALGASAPLAAETSAEWRQGAVEFTTPQG
                     AQAVMIRLSRAPCPDGACPIFGKVWYDDFDLQRVGGQPVAR"
     gene            complement(2186..3988)
                     /locus_tag="AC159P_16325"
     CDS             complement(2186..3988)
                     /locus_tag="AC159P_16325"
                     /inference="COORDINATES: protein motif:HMM:NF025104.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HIR3697061.1"
                     /translation="MPFFDSQHLRPVCVSFALALVLTPLVRALARRYKMVAKPKADRW
                     HSKPTAMMGGVAIYFAVALTYVFFIPHTPAGWVVMAASTFLFFVGLVDDVLHIKPYQK
                     LIGQVMGASVVVYFGLSLPWGWAASLSMAVTIFWLIGITNAINLLDNMDGLAAGVSAI
                     AAVFIAASLAANGQTTEAVMLGVFAAALLGFLVFNSNPATIFMGDAGSMFVGFFLAST
                     ALLSAAGGRSRSFLVVVAVPVLVLFIPIFDTTLVTVVRKLSGRAASQGGRDHASHRLV
                     ALGLSERRAVWMLYGFAGASGVLAMMVRELTPTASLALIVGFTVVLTLVGVYLAGVKV
                     YDETEVQAAREKPLVSFLVDLSYKRRIFEVLLDLVLITLAYYSAYTLLYGPAWPLGLR
                     QHFMQTIPVLIFVKMSALLVAGVYRGMWRYISLDYLIVYAKAVAAGSVASVLVLLFAF
                     RFEGFSRAVFVIDALVFFMMLAGSRVAFRLFRQMMPTPTNGGRRVLIYGAGDAGELLL
                     REMRNNLQLQYTPVGFVDDDPHKKGKVIHGLRVFGGNGNLRHICDQQRVEEVLISSQK
                     IADERVKQILRDCDAAQITLKRMRIEIELVGHEY"
     gene            4184..4423
                     /locus_tag="AC159P_16330"
     CDS             4184..4423
                     /locus_tag="AC159P_16330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697062.1"
                     /translation="MRRTFAAAAAAVLFALALVLPSPHAVRTVRADPPDPCTKCLGKV
                     QHEYEKCQARFGPVLMCDEEFNQGIVNCFATVCEL"
     gene            complement(4567..5547)
                     /locus_tag="AC159P_16335"
     CDS             complement(4567..5547)
                     /locus_tag="AC159P_16335"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HIR3697063.1"
                     /translation="MRVLITGGAGFIGSHLSDAYVARGDEVFVIDDLSTGSIDNIAHL
                     KPHPRFHYTIDSVHNQPLVAELIDQCDVVFHLAAAVGVKLIVESPVRTIETNVRGTEV
                     VLAQAKKKKKKVLVASTSEVYGLSMEVPFREDGNLVMGATTKGRWSYACSKAIDEFLA
                     LAYWREKKLPSVIVRLFNTVGPRQTGQYGMVIPTFVRQALAGRPVSVYGTGEQTRCFG
                     YVGDIVGALVKLMDKDEAVGQVFNIGSNEEVSIMELARRVKEMTGSTSEIVTVPYDEA
                     YEEGFEDMPRRVPDISKINALVGFRPTMTLDGILQSVIEYYSGRQTAHGS"
     gene            complement(5551..6882)
                     /locus_tag="AC159P_16340"
     CDS             complement(5551..6882)
                     /locus_tag="AC159P_16340"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR3697064.1"
                     /translation="MKVVRIIARLNVGGPARHVAWLEAGLRRAGIESLLVTGTVPPGE
                     DDMGYFAAGLGVTPVVIPEMSREVSPKDFVTVWKLYRLLRRERPDIVHTHTAKAGTVG
                     RAAGLLYRWLTPSALAARPRPCRFVHTYHGHIFHSYYGRWKTRFFLLVERLLARLVTD
                     RLVVISPQQYREIHEEFGVGRAGQFAVVPLGLDVAAFDNWAVRRHAARVELGAGEGDI
                     LVGIVGRLTEVKNHELFLHAVARYKKMARTGEAAAERRVRFYVIGDGHRREELEMLAH
                     ALGVEEAVTFTGLRDDPENFYPALDIVALTSRNEGTPLTLIEAMANARAVAATAVGGV
                     VDLLGGPDESAFRHHRAWRVCERGVRVQPGDAESFAHALAYLVEGDELRGQLGERGRR
                     FVEENYSVGRLLADVRGLYEDLTNPPRSDAARASRTTADRVPRAAARLKGD"
     gene            complement(6949..>7518)
                     /locus_tag="AC159P_16345"
     CDS             complement(6949..>7518)
                     /locus_tag="AC159P_16345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HIR3697065.1"
                     /translation="NYTYQEGEGGIADALGLCEDFADGEPVCVVLGDNIIEKNICQAA
                     KSYRHQGGGAKILLKKVTDPQRFGVPELDGRRVVRIEEKPTEPKSDYAVIGIYMYDAE
                     VFKIIKTLVPSGRGELEITDVNNAYIDRGEMTWDELDGWWTDAGTFESLLRASNLVAE
                     TGANNMEGAPARTASAAAAEKVWSLESGV"
BASE COUNT         1244 a         2607 c         2545 g         1122 t
ORIGIN      
        1 ccaccatcgc gatgatgccg ccgcgcgagt tggtcatcac gagcgcgacg gagcacagga
       61 gcgccgcgaa ggcgtagagc gggatgcggt cgcggtctat cgcgcccgag aagagcaggc
      121 ccaaaggcat cgccagcgcc agctccatgt agcccgcgaa gtggtggcgg ttgatgtagg
      181 ggccgaacgg ctgcgcctgc ttcggctcgc gcacccagta gatgacgtac gggttgagga
      241 agtgctgcat cagcccgaag agcgcgagca cgaagccgaa gatgatgacg gtgcggacga
      301 cgagccgcag gcggcgcggg ctgtcgatga aggcgagcgc ggcggcgaag tagacgaaga
      361 gcgccgagag ctggatgagc accagccgcg tcgcgtacgg gtcgaacgag agcgcgcgcg
      421 caggctcgac cgacagcccc gccgcgccga tgttctccgc gcccccggcg ccgagcggta
      481 ggagctgcac gaggccgagc agcgcgagtc cgaggagcgg cacttgcagg aagctcttgc
      541 tgaagcgcag gctccgcgtg cgccacgcgt cgatcatcca gagcgcgacc accacccccg
      601 cgctcaactg gaaggccgcg agcgaccacg cgtggacggt gccgaaggcg agcgtcgtcg
      661 tcaccaccgc gaggcacagc aggaggatga tgaagcggct ccagatggtg cgcggcgccg
      721 cgctttccgc gctcggcgcg tacggcggcg gcggcgcggg cggcggcgcg agcagttccg
      781 ccgagggcgc cgcgtcagcg tgcgaccggc tgcccgccga cgcgttggag atcgaagtcg
      841 tcataccaga ctttaccgaa gatggggcac gcgccgtcgg ggcacggcgc gcggctcaga
      901 cgaatcatca cggcctgcgc gccctgcggc gtcgtgaact cgaccgcgcc ctgccgccac
      961 tcggcgctcg tctcggcggc gagcggcgcg gacgcgccga gcgcggcccc gctcgcgccg
     1021 tccaagactt cgacgacggg cgtgctcgcg ctcttcaaat cctccgcgcg cgtgacgaag
     1081 ccgaggcggt agcgcgtcgc cggctcgacc gtgacgagtt gcaggacgcc tttgaagtcc
     1141 acgtgcatcg gcacgttgaa ggagaggcgc aagctgcgcg cgccgccgtg cttcacgcgc
     1201 ggatcgacgg cgacctgcac gccttcgggc ggcacgacct gccagccgaa atacttcttg
     1261 cccgcgggcg cgatgtccgc ctcgaagccg ccgttgccga ccgccccttc agccgcgccc
     1321 tcgtcgcccg cctcgcgcaa gacctgcgac gccgcgcggt acagcttctt cccgaacagc
     1381 gccttcacca gcatctcgcc cgcacccccg cggttcgcac gcttctcttc cgcgctcagg
     1441 cccgcccaga gcgcgagcgc ttcgtccagc aacccgcgcc cgaccagcac catcgccaac
     1501 tgcgcgcgcg ccgtcggcga gtcgccgatg ctcgccacga cgcgcttcac gtcgcggtcg
     1561 tagacctgcc acgcgaggtt gaagacctgc ggtcgcaagc cggcgtccgc gtccgccgcg
     1621 cgccttagct ccgcgaaggc ttcatccacc tgccccgcgc gcaggagcac gttgccgaga
     1681 tgccagcgcg gctgcgcgta ggacggcgcc agctcggtcg cgcggcgtag cgcgcggatg
     1741 ccgccctcgg cgtcgcccgc cgaagcccgc gcgcgcccca gctccatcca gatcaaatag
     1801 tcgttcggcg ccagcgccgc cgcccgctcg tactgcctga gagcctccgg cagctcctcc
     1861 ggcagcaggc tcacgcggcg cagacgcgcg agctgcaaac gcgcgtacgg gtcgtccggc
     1921 gcgagcctct cggcggactc ccccgtctcg aagtcccgcg ccgactccgc catcgtgttc
     1981 cccaccagcc aacgcacccc gcaccacgcc gcgaacagcg ccagcaccgc gacgagcatc
     2041 agcggcacgc gcaagagcag gggacgaacg ttgatgaatg ctatctgatc cgacatctat
     2101 gggtagccac cagccagtat ccggtagtca gtcgaagaac agctttcatg ctgcctactg
     2161 cctactgcct accgcctact tcttttcagt attcgtgccc caccagctcg atctcgatgc
     2221 gcatgcgctt gagcgtgatc tgcgccgcgt cgcagtcgcg gaggatttgt ttgacgcgct
     2281 cgtcggcgat cttctggctg gagatgagca cctcctcgac gcgctgctgg tcgcagatgt
     2341 ggcgcaggtt gccgttgccg ccgaagacgc gcaggccgtg gatgaccttg cccttcttgt
     2401 gcgggtcgtc gtccacgaag ccgacgggcg tgtactgcaa ctgcaagttg ttccgcatct
     2461 cgcgcagcag cagctcgccc gcgtcgcccg cgccgtagat caggacgcgc cgcccgccgt
     2521 tggtcggcgt cggcatcatc tgccggaaga ggcggaaggc gacgcggctc cccgccagca
     2581 tcatgaagaa gacgagcgcg tcgatgacga agacggcgcg cgagaagccc tcgaagcgga
     2641 aggcgaagag cagcaccagg acgctggcga ccgagccggc ggcgaccgcc ttcgcgtaga
     2701 cgatcaggta gtcgaggctg atgtagcgcc acatcccgcg gtagacgccc gccaccagca
     2761 gcgccgacat cttgacgaag atgaggacgg gaatcgtctg catgaagtgc tgccgcaggc
     2821 cgagcggcca cgccgggccg tagagcagcg tgtaggcgct gtagtaggcg agcgtgatga
     2881 gcaccaggtc gagcagcacc tcgaagatgc ggcgcttgta cgacagatcg acgaggaacg
     2941 agacgagcgg cttctcgcgc gccgcctgca cctcggtctc gtcatagacc ttgacgcccg
     3001 cgaggtagac gccgaccagc gtcaggacca ccgtgaagcc gacgatgagc gcgaggctcg
     3061 ccgtcggcgt cagctcgcgc accatcatcg ccagcacgcc cgacgcgccc gcgaacccgt
     3121 agagcatcca caccgcgcgc cgctccgaga ggcccagggc gaccagacgg tgcgacgcgt
     3181 ggtcgcgccc gccctgcgag gcggcgcggc ccgagagctt gcgcaccacc gtcacgagcg
     3241 tcgtgtcgaa gatcgggatg aagaggacga ggacggggac ggcgacgacg acgaggaagc
     3301 tgcgcgagcg cccgcccgcc gcgctcagca gcgccgtgct cgccagaaag aagccgacga
     3361 acatcgaccc ggcgtcgccc atgaagatcg tggccgggtt cgagttgaag accagaaagc
     3421 ccagcagcgc cgcggcgaag acgccgagca tcacggcctc ggtggtctgc ccgttggcgg
     3481 cgaggctcgc cgcgatgaag accgccgcga tggccgagac acccgcggcc agtccgtcca
     3541 tgttgtcgag caggttgatg gcgttcgtga tgccgatgag ccagaagatc gtcaccgcca
     3601 tgctcaggga ggcggcccag ccccacggca gcgagaggcc gaagtagacc accacgctcg
     3661 cgcccatcac ctgcccgatc agcttctggt aaggcttgat gtggagcacg tcgtccacca
     3721 gcccgacgaa gaagaggaac gtgctcgccg ccatgacgac ccacccggct ggcgtgtgcg
     3781 ggatgaagaa gacgtaggtg agcgcgaccg cgaagtagat ggcgacgccg cccatcatcg
     3841 ccgtcggctt cgagtgccag cggtcggcct tcggcttggc caccatcttg tacctgcgcg
     3901 ccagcgcgcg caccagcggc gtcaggacga gcgccagcgc gaacgagacg cagaccggcc
     3961 ggaggtgttg gctgtcgaag aaaggcacgg cggtacggtt ccgtaaaacg ggtgtcttgg
     4021 gtcgcgtaga agctattaga accgggcgga gaaatcaagt gcgcccgccc gcggcgcggg
     4081 gcgcggggcg cgggcgggcg ttgactttga ccgggattaa ttatagtaaa gccccgcctt
     4141 gaaagagtcc tcgcctcgaa cccaatcccg aaaggaacct accatgagaa ggacattcgc
     4201 cgccgccgcc gcggcagtct tgttcgcgct ggcgctcgta ctcccttcac cccacgccgt
     4261 gcgcaccgtc agggccgacc cgcccgaccc gtgcacgaag tgcctcggga aagtccagca
     4321 cgaatacgag aagtgtcagg cccggttcgg gccggtcttg atgtgcgacg aggagttcaa
     4381 ccagggcatc gtcaactgct tcgccacggt ctgcgagctg taacgggccg tgcccccgcc
     4441 gcggcgcccc ggccccgcgg cacgacgaaa caaggggcga ggtctccgca cggcctcgcc
     4501 ccttcgcctt tcgtcgaacg tttccgacaa gccgaacact tcggtcgcgc gcttcgcgca
     4561 cgaaggctaa ctcccgtggg ccgtctggcg cccgctgtag tattcgatga cgctttggag
     4621 gatgccgtcg agggtcatcg tcgggcggaa gccgacgagg gcgttgatct tggagatgtc
     4681 ggggacgcgg cggggcatgt cctcgaagcc ctcctcgtag gcctcgtcgt aggggacggt
     4741 gacgatctcg gaggtcgagc cggtcatctc cttgacgcgc cgcgccagct ccatgatcga
     4801 gacctcctcg ttcgagccga tgttgaagac ctgcccgacc gcctcgtcct tgtccatcaa
     4861 tttaacgagc gcgccgacga tgtcgccgac gtagccgaag cagcgcgtct gctcgcccgt
     4921 gccgtagacg ctgacggggc gcccggcgag cgcctgccgg acgaaggtcg ggatgaccat
     4981 cccgtactga ccggtctggc gcgggccgac ggtgttgaag aggcggacga tgaccgacgg
     5041 cagtttcttc tcgcgccagt aggcgagcgc gaggaactcg tcgatcgcct tcgagcaggc
     5101 gtaagaccag cgccccttgg tcgtcgcgcc catcacgagg ttgccgtctt cacggaaggg
     5161 tacttccatc gagaggccgt agacctcgga agtcgaggcg acgagcacct tcttcttctt
     5221 ctttttggcc tgcgcgagca cgacctccgt gccgcgcacg ttggtctcga tggtgcgcac
     5281 cgggctttcg acgatgagct tgacgccgac cgccgccgcg aggtggaaga ccacgtcgca
     5341 ctggtcgatc agctcggcga cgagcggctg gttgtggacg ctgtcgatgg tgtagtggaa
     5401 gcgcgggtgc ggcttgaggt gcgcgatgtt gtcgatgctg cccgtggaga ggtcgtcgat
     5461 gacgaagacc tcgtcgccgc gcgcgacgta cgcgtccgaa agatgcgagc cgatgaagcc
     5521 cgcgccgccc gtgattagaa ctcgcaaatg tcagtcccct ttcaatcgcg ccgccgcgcg
     5581 cgggacgcgg tcggcagttg tgcgcgaagc gcgtgccgca tcactccgcg gcgggttcgt
     5641 caggtcttcg tacagcccgc gcacgtccgc gagcagccgc ccgaccgagt agttctcttc
     5701 gacgaagcgg cggccccgct cgccgagctg cccgcgcagc tcgtcgccct cgacgaggta
     5761 cgcgagcgcg tgcgcgaagc tttcggcgtc gcccggctgc acgcgcacgc cgcgctcgca
     5821 gacgcgccac gcgcggtggt ggcggaacgc ggactcgtcc ggcccgccca ggagatcgac
     5881 gacgccgccg accgccgtgg ccgcgacggc gcgcgcgttc gccatcgcct cgatgagcgt
     5941 cagcggcgtc ccctcgttgc gcgaggtgag cgcgacgatg tcgagcgccg gatagaaatt
     6001 ctcggggtcg tcgcgcaggc ccgtgaacgt gaccgcctct tcgacgccga gggcgtgcgc
     6061 cagcatctcc agctcttcgc gccggtggcc gtcgccgatg acgtagaagc gcacgcgccg
     6121 ctcggcggcg gcctcgcccg tccgcgccat cttcttgtag cgcgcgaccg cgtgcaggaa
     6181 cagctcgtgg ttcttgacct cggtcagccg cccgacgatg ccgaccagaa tatcaccttc
     6241 gcccgcgccc aactcaacgc gcgcggcgtg ccggcgcacg gcccagttgt cgaacgccgc
     6301 cacgtcgagg ccgagcggca cgaccgcgaa ctggccggcg cgccccaccc cgaactcctc
     6361 gtgaatctcg cggtactgct gcgggctgat gacgacgagg cggtcggtca cgagccgcgc
     6421 gagcagccgc tcgacgagga ggaagaagcg cgtcttccac ctgccgtagt agctgtggaa
     6481 gatgtggccg tggtaggtgt ggacgaagcg gcacgggcgc ggcctcgccg cgagcgccga
     6541 cggcgtcagc cagcggtaga gcagccccgc cgcgcgtccc accgtcccgg ccttcgccgt
     6601 gtgcgtgtgg acgatgtcgg gccgctcgcg ccgcagaaga cggtagagct tccacaccgt
     6661 cacgaaatcc ttcggcgaga cttcgcggct catctcgggg atgacgacgg gcgtgacgcc
     6721 taagcccgcg gcgaagtaac ccatgtcgtc ctcgcccggc ggcaccgtgc ccgtcacgag
     6781 cagcgactct atcccggcgc gcctgaggcc cgcctccagc cacgccacgt gccgcgccgg
     6841 cccgccgacg ttgaggcgcg cgatgatgcg aacgactttc ataagtctgg aggctggagt
     6901 ccggagtctg gagtcagaac ataaggcgcc cgtctttgtc tccggacttc agactccgga
     6961 ctccagactc cagactttct ccgcggcggc ggcgctcgcg gtgcgtgccg gcgcgccttc
     7021 catgttgttg gcgccggtct cggcgacgag gttcgaggcg cggagcagcg actcgaaggt
     7081 gccggcgtcg gtccaccagc cgtcgagttc gtcccacgtc atctcgccgc ggtcgatgta
     7141 ggcgttgttc acgtccgtga tctcaagctc gccgcgcccc gaaggcacca gcgtcttgat
     7201 gatcttgaag acctcggcgt cgtacatgta gatgccgatg acggcgtagt cggacttcgg
     7261 ctccgtgggc ttctcctcga tgcgcacgac gcggcgcccg tcgagttcgg ggacgccgaa
     7321 gcgctgcggg tccgtcacct tcttcaacag gatcttcgcg ccgccgccct gatggcggta
     7381 ggacttcgcc gcctggcaga tgttcttctc gatgatgttg tcgccgagca cgacgcagac
     7441 gggttcgccg tcggcgaagt cttcgcacag cccgagcgcg tcggcgatgc cgccctcgcc
     7501 ctcttgatac gtgtagtt
//
LOCUS       DBNGOH010000845         2633 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3243542, whole genome shotgun sequence.
ACCESSION   DBNGOH010000845 DBNGOH010000000
VERSION     DBNGOH010000845.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2633)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2633)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2633
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3243542"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1640)
                     /locus_tag="AC159P_16350"
     CDS             complement(<1..1640)
                     /locus_tag="AC159P_16350"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR3697066.1"
                     /translation="MEIKPGTRLGHYEIRSALGAGGQGEVYKAVDTTLDRPVVIKVLS
                     PEQTVRPASLARFEREARLASSLDHPNICTIHGLHESNGVRFIAMQYVEGRNVRELVA
                     GRPLELRSALSIVIQVADALAAAHARGIIHRDIKAGNVMVMESGKVKVLDFGLAKLLE
                     GGAEDTHDPNLTEMGVPYGTATYAAPEQARGQKVDHRADIFSTGVLLYEALTGTWPFK
                     GGSVVEVRYAVIYDTPKPIAEVRGEDSPVIQRLQGILDRVLSKEPDARYQRVEELRED
                     LRAVLREIDPHASQDIHFTGSIGSIPPRHRGGAGGALANRPAARVAAFVGAGVLVAAL
                     AFGAYKLFGGGGAPGGAPTINSLAVLPFTNVGDDPGAEYLSDGMTESLINSLSQLPSV
                     KVRSRNSVFQYKGRPADSQKIGKELDVRAVLTGRVEHRGEDLLVNVELIDTRDDSHIW
                     GERYARKLSDLVRLQEELSRDITEKLRLKLTGEEQRQLVKNYATNSEAYQLYLQGRYN
                     WNKRTAEGLQKGIEYFTKAIEKDPGYAPAFSGLADCYWL"
     gene            2044..>2633
                     /locus_tag="AC159P_16355"
     CDS             2044..>2633
                     /locus_tag="AC159P_16355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008511932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HIR3697067.1"
                     /translation="MRGANMKVVLVRALVSLACAVAFVAVVAGGAWAAADKASSSRGK
                     VAPKPLFRDPVYDGAADPVVVWNRRERKWFMLYTNRRAKATDAPGVEWVHGTRIGVAE
                     SADDGATWKYRGTAEIGYGPADYTHWAPDVVYHAGTYHMYLTVVPGIFKDWNATRDIV
                     HLTSGDLLKWKYESTLKLASDKVIDASVARLPDGSWR"
BASE COUNT          375 a          842 c          959 g          457 t
ORIGIN      
        1 agccagtagc agtctgcgag gcccgagaag gcgggggcgt agccggggtc tttctcgatg
       61 gctttggtga aatactcgat gcccttctgc aacccttcgg cggtgcgctt gttccagttg
      121 tagcggcctt gcaggtagag ctggtacgcc tcggagttgg tcgcgtagtt cttgacgagc
      181 tgtcgctgct cctcgcccgt caacttcaga cgcagcttct cggtgatgtc gcgcgagagt
      241 tcttcctgaa ggcgcacgag gtcggagagc ttgcgcgcgt agcgctcgcc ccagatgtgg
      301 ctgtcgtccc gcgtgtcgat cagctcgacg ttgaccagca ggtcttcgcc gcggtgctcg
      361 acgcgccccg tcaggacggc gcgcacgtcc agctccttgc cgatcttctg cgagtcggcg
      421 ggtcgcccct tgtactggaa gacggagttg cgcgagcgca ccttgaccga aggcagttgc
      481 gagaggctgt tgatgaggct ctcggtcatg ccgtccgaga ggtactcggc gccggggtcg
      541 tcgccgacgt tcgtgaaggg caggacggcg agcgagttga tcgtgggggc gccgcccggc
      601 gcgccgccgc cgccgaagag cttgtaggcg ccgaaagcga gcgcggccac cagcacgccg
      661 gcgccgacga aggcggccac gcgggccgcg ggccggttgg cgagcgcgcc gcccgcgccc
      721 ccgcggtggc gcggcgggat ggagccgatg ctgcccgtga aatgtatgtc ctggctcgcg
      781 tgcgggtcga tctcgcgcag cacggcgcgc aggtcttcgc gcagctcttc gaccctttga
      841 tagcgggcgt ccggctcttt cgagagcacg cggtcgagga ttccctgaag tcgctggatg
      901 acgggcgagt cttcgccgcg cacttcggcg atgggtttcg gcgtgtcgta gatgacggcg
      961 tagcgcacct cgaccacgct cccgcccttg aacggccacg tccccgtgag ggcttcgtag
     1021 agcagcacgc cggtcgagaa gatgtcggcg cggtgatcga ctttttgccc gcgcgcctgc
     1081 tcgggcgcgg cgtaggtcgc ggtgccgtac gggacgccca tctccgtcag gttcgggtcg
     1141 tgcgtgtcct cggccccgcc ctccaacagc ttcgccagac cgaagtccaa aaccttgacc
     1201 ttgcccgact ccatgaccat cacgttgccg gccttgatgt cgcggtggat gatgccgcgc
     1261 gcgtgggcgg cggccagcgc gtccgcgacc tgaatcacga tggagagcgc gctgcggagt
     1321 tcgagcgggc ggcccgcgac cagctcgcgc acgttgcgcc cctcgacgta ctgcatggcg
     1381 atgaagcgca cgccgttcga ttcgtgcagc ccgtggatgg tgcagatgtt cgggtggtcg
     1441 agcgaggagg cgagccgcgc ctcgcgctcg aagcgcgcga gcgaggcggg cctgacggtc
     1501 tgctcggggc tgaggacttt gatgacgacg gggcggtcga gcgtcgtgtc cacggccttg
     1561 tagacctcgc cctgcccgcc cgcgccgagc gccgagcgaa tctcgtagtg gccgagtcgc
     1621 gtgccgggtt tgatctccat gactgccgtg cgacgcgggc cagggcgcct gcctgtgtga
     1681 cgggtcggcg gtcgggttcg cgtcccctcc gggtcggttg actggccgcg ccagcccgct
     1741 catgaccgtg gttttcttcg cggcacatta tcatgacatc cccggcagat aaagaggcaa
     1801 tgttccggcg ggcggcgacg cgcgcgcgtt cggcggcagc agacccgtcg aacatcgttc
     1861 ttgcaatctg cgtggaatag gttataactt ctcccacttc cagaccgcag ccgccccgcg
     1921 cgaggggatt cccacgaggg tttcagtcag gcggagctac gcgtcttctc ggcagaagag
     1981 gagggcgcgg cctcggtcgg cgggagcgcc ggaagggcgg gccgcaggag ggtctttttc
     2041 ctgatgcgag gagcgaacat gaaggtcgtg ctggtgcggg cgctcgtgtc tttggcgtgc
     2101 gcggtcgcgt tcgtcgccgt ggttgccggc ggggcgtggg cggcggcgga caaggcttcg
     2161 tcgtcgcgcg gcaaggtcgc gccgaagcct ctgttccgcg accccgtgta cgacggcgcg
     2221 gctgacccgg tggtcgtgtg gaaccggcgc gagcgcaagt ggttcatgct ctacacgaac
     2281 cgccgcgcga aggcgacgga cgcgccgggc gtcgagtggg tgcacggcac gcgcatcggc
     2341 gtcgcggagt cggcggacga cggcgcgacg tggaagtaca ggggcacggc ggagatcggc
     2401 tacggccccg cagactacac gcactgggcg ccggacgtgg tctaccacgc gggcacctac
     2461 cacatgtacc tgacggtcgt gcccggcatc ttcaaggact ggaacgcgac gcgcgacatc
     2521 gtccacctga cgagcggcga cctgctgaag tggaagtacg aatcgacgct gaagctcgcc
     2581 tccgacaagg tcatcgacgc ctcggtcgcg cgcctgcccg acggctcgtg gcg
//
LOCUS       DBNGOH010000846         6259 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3060605, whole genome shotgun sequence.
ACCESSION   DBNGOH010000846 DBNGOH010000000
VERSION     DBNGOH010000846.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6259)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6259)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6259
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3060605"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1686)
                     /gene="nifJ"
                     /locus_tag="AC159P_16360"
     CDS             complement(<1..1686)
                     /gene="nifJ"
                     /locus_tag="AC159P_16360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HIR3697068.1"
                     /translation="MHRKQITIDGNEAASHVAYKTNEVIAIYPITPSSNMGENADAWA
                     AEGRKNIWGLTPSVREMQSEGGAAGAVHGMLQTGSLTTTFTASQGLLLMIPNMFKIAG
                     EQTATVFHVAARAVATHALSIFGDHSDVMSTRSCGWAMLFANSVQEVLDFALVAQAAT
                     LRARVPFLHVFDGFRTSHEVQKIEQLTDEDVRALIDPELVRAHRARALTPDRPVMRGT
                     AQNPDVFFQSREASNPIYLATPFIVQETFDKFAAVTGRQYRLFDYVGDPAAERVVVMM
                     GSGCETAEETVLALRARGERVGLVKVRLYRPFSAEHLLAALPKTVRAIAVLDRTKEPG
                     AAGEPLYQDVLTAVGEAVIEDASPFAAIPRITGGRYGLASKEFTPAMAKAVFDELKKP
                     APKNHFTIGIKDDVTHTSLDFDRSFSTERADEVRALFWGLGADGTVGANKNTIKIIGE
                     ETDNYAQGYFVYDSKKSGAMTVSHLRFGPRPIRSTYLVSRANFVAVHQFNFVEKYDVL
                     DAAEEGATFLLNSPHPQEEVWDRLPRPVQQQIIDKRLRFYSINAYDVAKQAGMG"
     gene            complement(1722..1931)
                     /locus_tag="AC159P_16365"
     CDS             complement(1722..1931)
                     /locus_tag="AC159P_16365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697069.1"
                     /translation="MATRDVSRKELLSSDMPGDRNKVRAILAGWGVEESSIRRLMRER
                     RTDAAGDREARDSEREPRPGKYAGN"
     gene            complement(2044..2562)
                     /locus_tag="AC159P_16370"
     CDS             complement(2044..2562)
                     /locus_tag="AC159P_16370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697070.1"
                     /translation="MFIATTDRSYLRTLAWLVLLAIAIFAIGSLLVRAQNDPTTVQLG
                     ISTSTTDEPSMREYRGVSLGMTAAESRRKLGEPADMSDQQDFYTFSETESAQIFYDAS
                     LKVMAISAHYVGDGAGTPNPKSVFGVEVQPRADGSVYKLVKYQRAGYWVSYNRTAGDV
                     PVVTVSMRKDTP"
     gene            3052..4428
                     /gene="hemN"
                     /locus_tag="AC159P_16375"
     CDS             3052..4428
                     /gene="hemN"
                     /locus_tag="AC159P_16375"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008240833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HIR3697071.1"
                     /translation="MIAIANELLKKYDTPGPRYTSYPTILHWDTTPSPVEWVESVARG
                     LDETEREGFGAAVYVHIPFCRSLCTYCGCNSRITQNASVGDPYVEAVLKEWGLYRERL
                     GSERPIPLAELHLGGGTPTFLSPSELERLIGGLLRDVRRTDDFEFSVESDPRVTTREH
                     LSTLARLGFTRLSLGIQDFDPRVQQAVNRVQGESQVRDVTADARAEGFTSVNYDLIYG
                     LPFQTARSIKLTVDAVRRLRPERIAFYAYAHVPWFKPGQRHFTEEDLPRGDEKRALYE
                     LGRAMLEEAGYREVGMDHFALEGDSLLQAARDGTMHRNFMGYTSRRVAPLIGLGVSAI
                     SDSWEAFAQNEKTVEEYQARVGLGRLPFFRGHKLTREDLVLRRHILSLMTRFRTDWSS
                     GDDYTPHLETVADRLAEPLADGLVRLRDSCCEITDAGRPFLRNICMAFDARLARNADA
                     AHQFSRTV"
     gene            4512..5513
                     /gene="hemH"
                     /locus_tag="AC159P_16380"
     CDS             4512..5513
                     /gene="hemH"
                     /locus_tag="AC159P_16380"
                     /inference="COORDINATES: protein motif:HMM:TIGR00109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - protoporphyrin ferrochelatase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HIR3697072.1"
                     /translation="MKTGIALLNFGGPWTIGDVKPFLYRLFANPSVLVGVPSPLRQLI
                     AFTIAQVKGPSSVECYRLIGGGSPQLMWTEEQAEGLRRLLNGGGKGISSGGHANGDEA
                     RVEIGMRSAEPSIESALRKLKDWGAGRLVVLPLFPQFSTTTTGTCLQEVRDSLRRLRW
                     SPLTHEVRNWPDHRAYAALLRRTVDESVAQAERERGDSNDTIHVLFSAHSLPLKIVER
                     GDPYPQDVRRTVEAVAAGLGHPWSLCYQSRNGKLPWLQPYTEDELRRLGGEGVRRIVI
                     VPLSFVSDHVETLFELDLLYRDHALSHGIKHYYRARAFNGDPEFPLVLHSILKEARV"
     gene            5510..>6259
                     /locus_tag="AC159P_16385"
     CDS             5510..>6259
                     /locus_tag="AC159P_16385"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013739.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen/coproporphyrinogen oxidase"
                     /protein_id="HIR3697073.1"
                     /translation="MIGTFDTARREATVVGAGIAGMLAAYALDRKGYRVTLIEEQERA
                     GGLIQTMHTEYGMAERAAHSLLASPEVVEFCRELGVELAEIRPDSRARFIVRDGRLRK
                     FPLGFGETLGALKRAAFARAGAGADSHDLDAWGRTHLGDAAVEYLLTPFVRGIYGTQP
                     AELGVEAAFPKLSVSPGRTLLGTMLGKALRRDANSNGDASGHVKMDGHTNGAGPKKRG
                     KERKRMVAPLRGMGDVTARLERRLEERLGGRF"
BASE COUNT         1049 a         2054 c         2091 g         1065 t
ORIGIN      
        1 gcccatgccg gcctgtttcg ccacgtcgta ggcgttgatc gagtagaagc gcaggcgctt
       61 gtctatgatc tgctgctgca cggggcgcgg caggcgatcc caaacttcct cctgcgggtg
      121 cgggctgttc agaaggaatg tcgcgccctc ttccgccgcg tcgagcacgt cgtacttctc
      181 gacgaagttg aactggtgga cggcgacgaa gttggcgcgc gagacgaggt aggtgctgcg
      241 gatgggacgg gggccgaagc gcaggtgcga gaccgtcatc gcgcccgact tcttggagtc
      301 gtagacgaag tagccctgcg cgtaattgtc ggtctcctcg ccgatgatct tgatcgtgtt
      361 cttgttggcg ccgacggtgc cgtccgcgcc gaggccccag aagagcgcgc gcacttcgtc
      421 ggcccgctcg gtcgagaatg accggtcgaa atcgagcgag gtgtgcgtca cgtcgtcctt
      481 gatgccgatg gtgaagtggt tcttcggggc gggtttcttc agctcgtcga aaacggcctt
      541 cgccatcgcg ggcgtgaact ctttggaggc gaggccgtag cgaccgcccg tgatgcgcgg
      601 gatggcggcg aagggcgaag cgtcttcgat gaccgcctcg ccgacggcgg tcagaacgtc
      661 ctggtagagc ggttcgcccg ccgcgcccgg ctccttcgtg cggtcgagca cggcgatggc
      721 ccgcaccgtc ttcggcaacg ccgccagcag gtgctcggcg gagaacgggc ggtagagccg
      781 caccttgacg aggccgacgc gctcgccccg cgcgcggagc gccagcaccg tctcctctgc
      841 ggtctcgcag cccgatccca tcatcacgac gacgcgctcg gctgcggggt cgccgacgta
      901 atcgaagagg cggtactgac ggcccgtgac ggcggcgaac ttatcgaagg tttcctgcac
      961 gatgaagggg gtcgccagat agatcgggtt cgacgcctcg cgcgactgga agaacacgtc
     1021 ggggttctgc gccgtgccgc gcatgacggg gcggtcgggc gtgagcgcac gcgcgcggtg
     1081 cgcgcggacg agttcggggt cgatgagggc gcgcacgtcc tcgtccgtga gctgctcgat
     1141 cttctggact tcgtgcgagg tgcgaaaacc gtcgaagacg tggaggaagg gaacgcgcgc
     1201 acgcaacgtc gcggcctgcg cgacgagcgc gaagtccagc acctcctgaa cgctgttggc
     1261 gaacagcatc gcccagccgc aagagcgtgt gctcatcacg tccgagtggt cgccgaagat
     1321 cgaaagcgcg tgcgtggcga cggcgcgggc ggcgacatgg aaaacggtcg cggtctgctc
     1381 gcccgcgatc ttgaacatgt tcgggatcat caggagcagc ccttgactgg ccgtgaaggt
     1441 ggtcgtcaaa gagccggtct ggagcatgcc gtggacggcc cccgcggcac cgccctccga
     1501 ctgcatctcg cgcaccgacg gcgttaagcc ccagatgttc ttgcgccctt cggcggccca
     1561 cgcgtcggcg ttctcgccca tgttcgagga gggcgtgatc gggtagatgg cgatgacctc
     1621 gttggtcttg taggcgacgt gggaggccgc ctcgttgccg tcaatggtga tctgcttgcg
     1681 gtgcatcgtg tgtactctcg actgtggttg ggccgggccg ctcagttgcc ggcgtacttt
     1741 ccgggccgcg gctcgcgctc cgagtcccgc gcctcgcggt cgcccgccgc atctgtgcgg
     1801 cgctcgcgca tcaggcgacg gatcgaggac tcttcgacgc cccacccggc gaggatcgcc
     1861 cgaaccttgt tcctgtcacc cggcatgtcg ctcgaaagca actccttccg gctcacgtct
     1921 ctcgtcgcca tgacatcctc cttccgcgga cttcacgtcg gggcggggct attggaactg
     1981 cggcgtagtg tgcgaggcgc gtccgggaat gtttcgagtc ggacgcgcct cgcgcgcggc
     2041 cagctacggc gtgtccttcc gcatcgacac ggtgacgact ggcacgtcgc ccgcggtgcg
     2101 gttgtatgag acccagtagc cggcgcgctg gtatttgacg agcttgtaca cggagccgtc
     2161 cgcccgtggc tgcacctcca cgccaaaaac gcttttcggg ttcggcgtgc cggccccgtc
     2221 gcccacgtag tgtgcggaga tggccatcac cttgagcgag gcgtcgtaga agatttgtgc
     2281 cgactcggtc tccgagaagg tgtagaagtc ctgctggtcg ctcatgtcgg caggctcgcc
     2341 caacttccgg cgggactctg cggcggtcat ccccaggctc acgcccctgt attcacgcat
     2401 ggacggctcg tccgtggtgg acgtgctgat tcccaactgc actgtggtcg ggtcgttctg
     2461 tgcgcggacg aggagtgagc cgatggcgaa gatggcgatg gcgagcagga cgagccaggc
     2521 aagcgtcctg agataactgc ggtctgtcgt cgcaatgaac atcgtttccg gcctcctttc
     2581 gggaagcgcc cgccgaggcg ggtgctccac ctcacacacg aacatcgcaa acaacgtgcc
     2641 gactttcgtt gcggggaacg aaggctgaat tgcaggtact tttgacggcg acggcgtgag
     2701 acacgcttcg tacccatccg gtaatgggga attctccacc ggcgggtcgg taaattttct
     2761 cagtcacggc gcggagcgga cgcccactcg cgcacccgct tcccagaggc acgatggttg
     2821 cacccttcgc gccgtgtcag caccgcgggc gagccgtgaa ggagaagtcg agcgacatga
     2881 gccgggtatt gacgggggaa ttggaagctg ccggaagtcg caccgcataa agcagcagac
     2941 atcgacagcc ggggcgactc aagggaagtg cggattattc cgcagccatg agaagaaaac
     3001 ttcgcagggt atggccgagt cctcggagac atgactcgaa gaaagcaact tatgatcgcg
     3061 atcgctaacg agcttctgaa gaagtacgac acgcccggcc cgcgctacac gagctacccc
     3121 accatcctcc actgggacac gacgccttcg cccgtggagt gggtggaatc ggtcgcgcgc
     3181 gggctggacg agaccgaacg ggaaggcttc ggcgccgccg tctatgtcca catccccttc
     3241 tgccgctcgc tctgcaccta ttgcggctgc aactcgcgca tcacgcagaa cgcgtcggtc
     3301 ggcgaccctt atgtcgaagc cgtgctgaag gagtgggggc tgtaccgcga acgcctcggc
     3361 agcgaaaggc cgatcccgct cgccgaactg cacctcggag gcggcacccc gaccttcctt
     3421 tcgccctcgg agcttgagcg cttgatcggc ggcttactcc gcgacgtgcg ccgcacggac
     3481 gacttcgagt tctccgtcga gtccgacccg cgcgtgacga cccgcgaaca cctctcgacg
     3541 ctcgcccgcc tcggcttcac acgcctgagc ctcggcattc aggacttcga cccccgcgtg
     3601 cagcaggccg tcaaccgcgt tcagggcgag agccaggtgc gcgacgtcac cgcggatgca
     3661 cgcgccgaag ggttcacgag cgtcaactac gaccttatct acggcctgcc gttccagacc
     3721 gcccgaagca tcaagttgac cgtggacgcc gtccggcggc tgcgccccga gcgcatcgcc
     3781 ttctacgcct acgcgcacgt cccctggttc aagccgggcc agcgccactt caccgaagaa
     3841 gacctcccgc gcggcgacga gaagcgcgcg ctctacgagc tgggccgcgc gatgctcgaa
     3901 gaggcgggct accgggaggt cggcatggat cacttcgcgc tggaagggga ttcgcttttg
     3961 caggccgcga gagacgggac gatgcaccgc aacttcatgg gctacacctc gcgccgcgtc
     4021 gcgccgctca tcggcctcgg cgtctccgcg atcagcgact cgtgggaggc gttcgcgcag
     4081 aacgagaaga cggtcgagga gtatcaggcg agagtcggac tcgggcgtct tccattcttc
     4141 cgcggtcaca agctcacgcg cgaagacctc gtgctccgcc gccacatcct gagcctgatg
     4201 acgcgctttc gcaccgactg gtcttcgggc gacgactaca cgccccacct cgaaacggtc
     4261 gccgacaggc tcgccgagcc gctcgcggac ggcctcgtca gactccgcga ctcgtgctgc
     4321 gagatcactg acgcggggcg gcccttcctc cgcaacatct gcatggcctt cgacgcgcga
     4381 ctcgcccgca acgcggacgc cgcgcaccag ttcagccgga cggtctgaag gaaacgcgga
     4441 atgatgaatg atgaatcgaa tcaggccggc ctcgttacat tccgcattca tcattccgca
     4501 ttcatcattt tatgaaaaca ggcatcgcgc ttcttaattt cggcggcccg tggacgattg
     4561 gcgacgtgaa gcctttcctc taccggctct tcgccaaccc gtcggttttg gtcggcgtgc
     4621 cttcgcccct gcgtcagctc atcgccttca ccatcgcgca ggtgaagggg ccttcgtcgg
     4681 tcgagtgcta cagactgatc gggggcggct cgccacagct catgtggacc gaagagcagg
     4741 ccgaaggctt gcggcgtttg ctgaacggcg gcgggaaggg catcagcagc ggcggccacg
     4801 cgaacggcga cgaggcgcgc gtcgagatcg ggatgcgcag cgcggagcct tccatcgaat
     4861 ccgcgctccg gaagttgaag gactggggcg cgggccgtct cgtcgtgctg ccgctcttcc
     4921 cgcagttctc gacgacgacg accgggacat gtttgcagga ggtgagggat tcgctgcggc
     4981 gcctgcgctg gtcgccgctc acccacgagg tcaggaattg gcccgaccac agggcttacg
     5041 ccgcgctgct gcgccgcacc gtggacgaat cggtcgcgca ggccgagcgc gagcgcggcg
     5101 actctaatga caccatccac gtccttttct ccgcacactc gctgccgctg aagatcgtcg
     5161 agcgcggcga cccgtatccg caggacgtgc ggcgcacggt cgaggcggtg gccgcgggcc
     5221 tcggacaccc gtggtcgctc tgctaccagt cgcgcaacgg caagctgccg tggctccagc
     5281 cctacacgga agacgaactc cggcgtctcg gcggcgaggg cgtgcggaga atcgtcatcg
     5341 tcccgctgag cttcgtcagc gatcacgtcg agacgctctt cgaactagac ctgctctacc
     5401 gagaccatgc actctcgcat ggcatcaagc actactaccg cgcgcgcgcc ttcaacggcg
     5461 accccgagtt ccctttggtt ctgcactcga tcctgaaaga ggcacgcgta tgatcggcac
     5521 attcgacacg gcgcggcgcg aggcgactgt ggtcggcgca gggatagccg gcatgctcgc
     5581 ggcctacgcg ctcgacagga agggctatcg cgtcacgctg attgaagagc aggagcgcgc
     5641 gggcgggctg attcagacca tgcacaccga gtacggcatg gccgagcggg cggcgcattc
     5701 gctgctcgcg tcgcccgagg tcgtcgagtt ctgccgcgaa ctcggcgtcg aactcgccga
     5761 aatccggccc gactcgcgcg cccgcttcat cgtgcgcgac gggaggctca gaaagttccc
     5821 gctcggcttc ggcgaaaccc tcggcgcgct gaagcgtgcg gcgttcgcac gcgccggggc
     5881 gggcgcggac tcgcacgacc tcgacgcgtg ggggcgcacg cacctcggcg acgcggcggt
     5941 cgaatacctc ctgaccccat tcgttcgcgg catctacggc acccaacccg ccgaactcgg
     6001 cgtcgaggcc gccttcccca agctctcggt ctcgccgggg cgaacgctcc tcggcacgat
     6061 gctcggcaag gcgctacgcc gcgacgcgaa cagcaacggg gatgcgagcg gccatgtgaa
     6121 gatggatggc cacacgaacg gcgccgggcc gaagaaacgc gggaaggagc gcaagcgcat
     6181 ggtcgcgccg ctccgcggga tgggcgacgt gacggcgcgg ctcgaaaggc gtctggagga
     6241 gcgactgggc gggcgcttc
//
LOCUS       DBNGOH010000847         3333 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4342450, whole genome shotgun sequence.
ACCESSION   DBNGOH010000847 DBNGOH010000000
VERSION     DBNGOH010000847.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3333)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3333)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3333
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4342450"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            197..2080
                     /locus_tag="AC159P_16390"
     CDS             197..2080
                     /locus_tag="AC159P_16390"
                     /inference="COORDINATES: protein motif:HMM:NF015506.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HIR3697074.1"
                     /translation="MKQCPTCQEEFADKFGFCPVDGTPLDGHVAAPEAFAAQQVGDES
                     VVTVAPTAATADSAAGSAADSSNGHGHVGEIPPSEATGGDDREEYHLTMLEDVGITRR
                     LVQQTRDFKEQTWPEFKRDPVGTSQRTAVAVGQATRRFFKQDYATAAIISGVLAMILT
                     IGILVFPYATVYKMFASDGKLVANIGMPLEEVQKLSTLKLAEPNGQADSTGATAISAE
                     NVDFDFELAGTGMRFDWSRSYMLQLDENRNVSKISIEVAQQNETWRELRHNMLDIAEW
                     LDVKGWRSISDDEGRSSVAYLNAAFEQSAPREGTVGEFRWMNEERGLVLTLSAVRETA
                     AKEGEDPTVAAQFVHFITVEKVPDAGQLELVTIIPKEQPTPDPGPAGTNKGTGGGSNP
                     KPEKPGGGGGGGREDPLPVSHGKVAPASMNPQIMPPSVRVPTITQPKLPVAATLKGDP
                     LLLPEDTRPIPFGDPKSTSTIPSDGPGKGGGQGTGTGTGQGPGDGAGYGPGRGENTGG
                     GDARYGGGGPGGGGGGAPPVDYNRVFNPKDVTSKAVIISKPDPGFTEEARKNNVTGVV
                     RLRAVLSAGGAVTNIGVVKGLPDGLTERAIAAARNIKFRPAQKDGRNVSQHITLEYNF
                     NIY"
     gene            2215..>3333
                     /locus_tag="AC159P_16395"
     CDS             2215..>3333
                     /locus_tag="AC159P_16395"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HIR3697075.1"
                     /translation="MADTPAAAVNVVCVASHYKGVEFLRECKRQGAHISLVTREKALG
                     EDWPRETLDDIVAVPDEAGTEVLLHAVGHLSRKRRPHAVVALEEFDVVNAALVREHLL
                     VPGMRATTARKFRDKLTMRVVADGAGIRVPEFVHALNYEEVGQFMERVPPPWVLKPRS
                     DVSAAGIRKLDEAEQVWRAIDELDARAALHDRSPYYLLERYVAGEVFHVDSLVEDGRV
                     VFAGASRYGRPPMDVMHGGGVFTSYTVEYDSKERGELMKANRKLLKSLGLESGAAHAE
                     FIKGAEDGQLYFLEVAARVGGAYIAETLEAASGVNLWREWARAEVARAAGAPPAAVEP
                     RTDYSGIALSLARQERPDTSAYDDAEIVYRITKPHHVGL"
BASE COUNT          575 a         1159 c         1150 g          449 t
ORIGIN      
        1 cggcgcaccg tttggcgcga acgcgcatcc aaattccgtt tcgagtaccc gtgcgcccca
       61 caggtacgca agcccgtgag acgtttggta taatcagttc aggcgccccc ggcgacagag
      121 ccgcgccggg gcgaggaacg aaccctcggc agagatttaa cctgctttcc cgtccggtag
      181 cagcgaggtt gtgaagatga aacagtgccc aacgtgccag gaagagtttg cagacaagtt
      241 cggtttctgt ccggttgacg ggacgccgct cgacggccat gtcgccgccc ccgaagcttt
      301 cgccgcgcag caggtcggcg acgagtcggt cgtcacggtc gctcccacgg cggccactgc
      361 ggactccgcc gcgggttccg ccgcggactc ttccaacggc cacggccacg tgggagagat
      421 tccgccctcg gaagcgaccg gcggcgacga ccgcgaagag taccacctga cgatgctgga
      481 ggacgtgggc atcacccgcc gcctcgtcca gcagacgcgc gatttcaagg agcagacctg
      541 gccggaattc aagcgcgacc ccgtcggcac ctcgcagcgc acggcggtcg ccgtcgggca
      601 ggccacgcgc cgcttcttca aacaggatta cgccaccgcc gccatcatca gcggggtgct
      661 cgcgatgatt ctcaccatcg gcatcctcgt cttcccgtac gccacggttt acaagatgtt
      721 cgcgtccgac ggcaagctgg tggcgaacat cgggatgccg ctcgaagagg tgcagaagct
      781 ctcgacgctg aagctggccg agccgaacgg gcaggccgac tcgacgggcg cgacggccat
      841 ctccgccgag aacgtggact tcgacttcga gctggccggg acggggatga ggttcgactg
      901 gagccgctcc tacatgctcc agctcgacga gaaccggaac gtctcgaaga tcagcatcga
      961 ggtcgcgcag cagaacgaga cctggcgcga gctgcggcac aacatgctcg acatcgccga
     1021 gtggctcgac gtgaagggct ggcggtcgat ttcggacgac gagggccgct cgtcggtcgc
     1081 ctacctgaac gccgcgttcg agcagagcgc gccgcgcgag ggcaccgtcg gcgagtttcg
     1141 ctggatgaac gaggagcggg ggctggtgct gacgctctcc gccgtgcgcg agacggcggc
     1201 caaagagggc gaagacccga cggtcgccgc gcagttcgtc cacttcatca cggtcgagaa
     1261 agtccctgat gccgggcagt tggaactcgt gacgatcatc ccgaaagagc agccgacgcc
     1321 cgaccccggc cccgcgggca cgaacaaggg cacgggcggc ggctccaacc cgaagcccga
     1381 gaagccgggc ggcggcggcg gcggcgggcg cgaggacccg ctgcccgtct cccacggcaa
     1441 ggtcgcgccc gccagcatga acccgcagat catgccgccg agcgtgcgcg tgccgaccat
     1501 cacgcagccg aagctgcccg tggcggcgac gctcaagggc gacccgctcc tgctccccga
     1561 ggacacgcgc ccgatcccct tcggcgaccc gaaatccact tcgaccatcc cctcggacgg
     1621 ccccggcaag ggcggcggac agggcacagg cacgggcacc gggcaggggc cgggtgacgg
     1681 cgcgggctac ggccccggta ggggcgagaa cacgggcggc ggcgacgcgc gctacggcgg
     1741 cggcgggccg ggcggcggcg gcggcggcgc gcccccggtc gattacaacc gcgtcttcaa
     1801 cccgaaggac gtgacgagca aggccgtcat catctcgaag cccgaccccg gctttaccga
     1861 ggaggcgcgc aagaacaacg tgaccggcgt cgtccggctg cgcgcggtgc tctcggcggg
     1921 cggcgcggtg acgaacatcg gcgtcgtcaa ggggctgccc gacggcctga ccgagcgcgc
     1981 catcgcggcg gcgcggaaca tcaagttccg ccccgcgcag aaggacggcc gcaacgtctc
     2041 gcagcacatc acgctcgaat acaacttcaa catctactga cgcgccgcgt ccgttgatga
     2101 tgtttcgaca gggcggctgc tcctacgcgg ggcagccgct tccttttggg ccggagccgg
     2161 tcatatactt gaccccgcaa ttccacccat cccggctgta gagaggaatc ccacgtggcc
     2221 gacacgccag ccgcagcggt caacgtcgtc tgcgtcgcca gccattacaa gggcgtcgag
     2281 ttcctgcgcg agtgcaaacg gcagggcgcg cacatctcgc tcgtgacgcg cgagaaggcg
     2341 ctcggcgaag actggccgcg cgaaacgctg gacgacatcg tcgcggtgcc ggacgaggcc
     2401 ggcaccgaag tcctgctcca cgccgtcggc cacctctcgc gcaagcgcag gccgcacgcc
     2461 gtcgtcgcgc tcgaagagtt cgacgtggtc aacgcggcgc tcgtgcgcga gcacctgctc
     2521 gtgcccggca tgcgcgcgac caccgcgcgc aagttccgcg acaagctgac gatgcgcgtc
     2581 gtggccgatg gggcgggcat ccgcgtcccc gagttcgtcc acgcgctcaa ctacgaagag
     2641 gtcggccagt tcatggagcg cgtgccgccg ccgtgggtct tgaagccgcg ctcggacgtg
     2701 tcggcggcgg gcatcaggaa gctcgacgag gccgagcagg tctggcgcgc gatcgacgag
     2761 ctggacgcgc gcgcggccct gcacgaccgc tcgccttact acctgctcga acgctacgtc
     2821 gcgggcgagg tcttccacgt cgattcgctg gtcgaagacg ggcgggtcgt cttcgcgggc
     2881 gcgagccgct acggtcgccc gccgatggac gtgatgcacg gcggcggcgt cttcacctcc
     2941 tacacggtcg agtacgactc gaaggagcgc ggcgagctta tgaaggccaa ccgcaagctg
     3001 ctgaagagcc tcgggctgga gagcggcgcg gcgcacgccg agttcatcaa gggcgcggag
     3061 gacggccagc tctacttcct cgaagtcgcg gcgcgcgtcg gcggcgcgta catcgcggag
     3121 accctggagg ccgcgagcgg cgtcaacctc tggcgcgaat gggcgcgcgc ggaagtggcc
     3181 cgcgcggcgg gcgcgccgcc tgccgccgtc gagccgcgca cggactatag cggcatcgcg
     3241 ctctcgctcg cgcggcagga gcgccccgac acctctgcct acgacgacgc cgagatcgtc
     3301 taccggatta ccaagccgca ccacgtcggc ctc
//
LOCUS       DBNGOH010000848         2716 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1960488, whole genome shotgun sequence.
ACCESSION   DBNGOH010000848 DBNGOH010000000
VERSION     DBNGOH010000848.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2716)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2716)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2716
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1960488"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2435)
                     /locus_tag="AC159P_16400"
     CDS             complement(<1..2435)
                     /locus_tag="AC159P_16400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011319522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGH1-like glycoside hydrolase domain-containing
                     protein"
                     /protein_id="HIR3697076.1"
                     /translation="MTSDGTDVLEEQASPTPDADDFEKRAGDDAGRAAATAEARRLEE
                     SRARTAHWKRWGPYLSERAWGTVREDYSPDGTAWGYLPHDHARSQAYRWNEDGLAGIC
                     DRHQLMCFALALWNGRDPLLKERLYGLTGEEGNHGEDVKEYYFYLDSTPTHSYMKYLY
                     KYPQGEFPYARLVEENRRRGKGAPEYELLETGVFDEDRYFDVTVEYAKGGVEDILVRV
                     SATNRGPEAAPLHLLPTLWFRNPWSWDEQPEGARPRLACVEGCGGEEGAESVAAVEAR
                     EEIYGTRWLYCEGTPELLFTENETNAARLYDAPNPTPFVKDGIGDYVVNDDPRAVNPA
                     QEGTKAAALYVLDIAPGETATVRLRLTDQSPASFVEVFGEEFERVFAERRREADEFYA
                     SVIPEDLTEDARNVMRQSFAGMLWSKQFYHYVVKQWLEGDPAQPAPPAERERGRNHDW
                     PHLYNADVISMPDKWEYPWYAVWDLAFHCVPLALVDPDFAKEQLILVLREWYMHPNGQ
                     IPAYEWAFGDVNPPVHAWAAWRVYKIDEKRHGKGDREFLERVFHKLLLNFTWWVNRKD
                     EDGNNVFQGGFLGMDNVGVFDRSQPLPTGGHLAQSDGTSWMAMYTLNLLAIALELAKT
                     DPVYEDVASKFWEHFLYIAHAMNNIGPDNIELWDEEDGFYYDVLHLHDQGSHALKVRS
                     MVGLIPLFAVETLEPEVLDKLPGFKKRLEWFIDNRPDLTGDVACMRTPGRGERRLLSV
                     VYRDRLRRVLRLMLDESEFLSPCGVRSVSRYHREHPYVLRVDGTEHRVDYEPAESSTG
                     LFGGNSNWRGPVW"
     gene            complement(2432..2626)
                     /locus_tag="AC159P_16405"
     CDS             complement(2432..2626)
                     /locus_tag="AC159P_16405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697077.1"
                     /translation="MPEEKRMVCPDCGVGMNHHADKLVYGGEEEESAGDGLGGVVEEA
                     HSCPKCGKTLTRRKGGDKAP"
BASE COUNT          409 a          940 c          888 g          479 t
ORIGIN      
        1 aaccagacgg ggccgcgcca gttcgagttg ccgccgaaga ggccggtcga actctcggcc
       61 ggctcgtaat cgacgcggtg ctcggtgccg tccacgcgca gcacgtacgg gtgctctcgg
      121 tggtagcgcg agacggagcg cacgccgcag ggcgagagga actccgactc gtcgagcatc
      181 agccgcagca cgcggcgcag ccggtctcga tagacgaccg agagcaggcg gcgctcgccg
      241 cggccgggcg tgcgcatgca ggccacgtcg ccggtcaggt cgggccggtt gtcgatgaac
      301 cattcgagcc gcttcttgaa gccgggcagc ttgtcgagca cttccggctc caaagtctcg
      361 accgcgaaga gcggtatcaa cccgaccatc gagcgcacct tgagcgcgtg gctgccctgg
      421 tcgtgcaggt ggagcacatc gtaataaaaa ccgtcctcct cgtcccacag ctcgatgttg
      481 tcggggccga tgttgttcat cgcgtgggcg atgtagagga agtgctccca gaacttcgag
      541 gccacgtcct cgtagaccgg gtccgtcttc gccagctcca gcgcgatggc cagcaggttg
      601 agcgtgtaca tcgccatcca gctcgtcccg tccgactgcg cgaggtggcc gcccgtgggg
      661 agcggctgcg agcggtcgaa gacgccgacg ttgtccatcc cgaggaagcc gccctggaag
      721 acgttgttcc cgtcctcgtc cttgcggttg acccaccacg tgaagttcag cagcagcttg
      781 tggaagacgc gttccagaaa ttcgcggtcg cccttgccgt gccgcttctc gtcgatcttg
      841 tagacgcgcc acgcggccca cgcgtggacg ggtgggttca cgtcgccgaa ggcccactcg
      901 tatgccggta tttgcccgtt cgggtgcatg taccactcgc gcaggacgag gatcaactgc
      961 tctttggcaa agtccggatc gacgagcgcg agcgggacgc agtggaaggc caagtcccag
     1021 acggcgtacc acgggtactc ccacttgtcg ggcatcgaga tcacgtcggc gttgtagagg
     1081 tgcggccagt cgtggttgcg gcctcgttcg cgctcggctg gcggcgcggg ctgcgccggg
     1141 tcgccttcga gccactgttt gacgacgtag tggtagaact gcttcgacca cagcatcccc
     1201 gcgaacgact gccgcatcac gttgcgcgcg tcctcggtga ggtcttcggg gatgacggag
     1261 gcgtagaact cgtcggcctc gcggcgccgc tccgcgaaga cacgctcgaa ctcttcgccg
     1321 aagacctcga cgaaggaggc gggcgactgg tcggtcagcc gcagccgcac cgtcgcggtc
     1381 tcgccgggcg cgatgtcgag gacgtagagc gccgccgcct tcgtcccttc ctgcgcgggg
     1441 ttgacggcgc gcgggtcgtc gttgaccacg tagtcgccga tgccgtcctt gacgaagggc
     1501 gtcgggttcg gtgcgtcgta gagccgcgcg gcgttcgtct cgttctccgt gaagagcagc
     1561 tcgggcgtgc cttcgcagta gagccagcgc gtgccgtaaa tctcctcgcg cgcctcgacg
     1621 gcggcgacac tctccgcccc ttcttcgccg ccgcaaccct cgacgcaggc gagacgcggg
     1681 cgcgcgccct ccggttgctc gtcccacgac caggggttgc ggaaccagag cgtcggcagc
     1741 aggtgcagcg gcgccgcttc ggggccgcgg ttcgtcgcgc tcacgcggac gaggatgtct
     1801 tccaccccgc ccttcgcgta ctcgaccgtc acgtcgaaat agcgatcctc gtcgaagacg
     1861 cccgtctcca aaagctcgta ctcgggcgct cccttgccgc gccgccggtt ctcctcgacg
     1921 agccgcgcgt aggggaactc cccttgcggg tacttgtaga ggtatttcat gtaggagtgc
     1981 gtcggcgtcg agtcgaggta gaagtagtac tccttcacgt cctcgccgtg gttgccctcc
     2041 tcgcccgtca gcccgtagag ccgctccttg aggagcgggt cgcgcccgtt ccagagcgcg
     2101 agcgcgaagc acatcagttg gtggcggtcg cagatgccgg cgaggccgtc ctcgttccag
     2161 cggtaggcct gcgagcgcgc gtggtcgtgc ggcaggtagc cccacgccgt gccgtcgggc
     2221 gagtagtcct cgcgcaccgt gccccacgcg cgctccgaga gatacggccc ccagcgcttc
     2281 cagtgcgccg tgcgcgcacg cgactcttcg agccgcctcg cctcggcagt cgccgccgcg
     2341 cgccccgcgt cgtcgcccgc gcgcttctcg aagtcgtccg cgtccggcgt cggcgacgcc
     2401 tgctcctcaa gcacgtcggt tccgtccgaa gtcatggtgc cttgtccccg cccttccgcc
     2461 gcgtcagcgt cttcccgcac ttcgggcacg agtgcgcctc ctcgacgacg ccccccagcc
     2521 cgtcgcccgc cgactcctcc tcctcgcccc cgtacaccag cttgtccgcg tggtggttca
     2581 tccccacccc gcaatcgggg cagaccattc gcttctcttc aggcataaac acctcgattg
     2641 acaaaggccg taagccgtga accgtaaacc gtgataagtg aaatgcgatg accggtgaca
     2701 agtgctaatt gacgag
//
LOCUS       DBNGOH010000849         3309 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_5076951, whole genome shotgun sequence.
ACCESSION   DBNGOH010000849 DBNGOH010000000
VERSION     DBNGOH010000849.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3309)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3309)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3309
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_5076951"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..251
                     /locus_tag="AC159P_16410"
     CDS             <1..251
                     /locus_tag="AC159P_16410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020475429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697078.1"
                     /translation="PPVTLEEMARGFAERLINLFKRNEEGLRPAHGWYAKFQHDPHWR
                     DLVLFYEYFHGDTGMGLGASHQTGWSGLVASLIDEWRK"
     gene            516..2444
                     /locus_tag="AC159P_16415"
     CDS             516..2444
                     /locus_tag="AC159P_16415"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HIR3697079.1"
                     /translation="MTLLQALAAQYPTENLALAEAAALRASLSFPKGVVHVVSDVHGE
                     YKKLRHIINNASGSLRPCVESLFEGEMTADELKQFLATIYYPHETVEHLRTSLVETGR
                     RGEWVSRTLRRQFKVARALAGNRRLEEIRALFPPERRQLFEELFFEPCTGRGESYVDE
                     MIHTLCRHDRDFSAVRAASRLVRNLSVAEIVVAGDLGDRGPRLDRVIDYLLQQPNVSF
                     TWGNHDVLWMGACLGHEALMATVLRVSLRYRRLSQLEEGYGITMQPLEKLARTVYAED
                     PAERFKTKGVGLRDDLLMARMQKAIAVIQFKLEGQVIRRHPVWELEHRNLLHRIDPAE
                     GTVEIQGQPHPLRDASLPTVNPADPYALTEEEATCVSRLRQSFVSSSRLWQHMSFVAR
                     RGAMWVRRDHALIFHGCVPVEEGGAALPLEVDGQTHSGRALFDALDLVVRRAFRKGSE
                     ATEVDKDWLWYLWTGPRSPLFGKDRMATFETYFVEDKATHKENKNPYFKLIHDPAFCA
                     RAAAEFGVTEQALIVNGHVPVRVEKGEEPVKSGGGAVTIDGAFSEAYGDRGYTLILAP
                     ERVALAEHHHFESVSDAITEGADIVPKVTTLRAYDPPRRVGDTEEGNPIRHTVAALER
                     LVEAYERGLLLEETDRPR"
     gene            2583..3074
                     /locus_tag="AC159P_16420"
     CDS             2583..3074
                     /locus_tag="AC159P_16420"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RrF2 family transcriptional regulator"
                     /protein_id="HIR3697080.1"
                     /translation="MKVSAQEEYGLRCLLQLAQLGEGEYLTLAQIAEREGISAANAGK
                     LLWILSKANLVSSIRGTKGGYRLARPSSEIHLSEIIKVLDEEDVLASHCKSYAGVLDT
                     CVHTGDCGIRPVIVGLHEIVRNALSGITLAQLIGTEAKVDQTIHRIQGLRPRVETAEG
                     RVQ"
     gene            3132..>3309
                     /locus_tag="AC159P_16425"
     CDS             3132..>3309
                     /locus_tag="AC159P_16425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HIR3697081.1"
                     /translation="MSSIEVLTNKEYKYGFVTDIESDMIPRGLTEDTVRLISEKKGEP
                     EWMLEFRLKAYRQWL"
BASE COUNT          616 a         1113 c         1042 g          538 t
ORIGIN      
        1 agccgcccgt cacgctcgaa gagatggcgc gcggcttcgc cgaacggctc atcaacctct
       61 tcaagcgcaa cgaagagggc ttgcgccccg cgcacggctg gtacgccaag ttccagcacg
      121 acccgcactg gcgcgacctc gtgctcttct acgaatactt ccacggcgac accggcatgg
      181 gcctcggcgc ctcgcaccag acaggctggt cgggcctcgt cgcatcgctg atagacgagt
      241 ggcggaagtg attctctgca cggcaaccag cccacccggt ggtcgagtgt tgtaagagcg
      301 ttgtttcaac gcagaggacg cagaggcgca gaggacgcag aggaaaacaa tctcggattc
      361 gagaatttgg gcttcgaagt ttcaaatcct cgatttcttc tctgcgttct ctgcgtccct
      421 ccgcgaactc tgcgttaaat ctcaccacgg gtaatcacgc atcctgcgaa gcaaatggga
      481 ggccgaaagt gagtgctcaa ttcgacgcct cggagatgac gctgctgcaa gcgctcgccg
      541 cgcagtatcc gacggaaaac ctcgcgctcg cggaggccgc cgccctgcgc gcctcgctct
      601 cgttcccgaa gggcgtggtg cacgtcgtca gcgacgtgca cggcgagtac aagaagctgc
      661 gccacatcat caacaacgcc tcggggagcc tgcgcccctg cgtcgaatcg ctcttcgagg
      721 gcgagatgac ggcggacgaa ctcaaacagt tcctcgccac catctactac ccgcacgaga
      781 cggtcgagca cctgcgcact tcgctcgtcg agacggggcg gcgcggcgag tgggtgagcc
      841 gcacgctccg ccgccagttc aaggtggcgc gcgcgctggc cggcaaccgc cgcctcgaag
      901 agattcgcgc gctgttcccg cccgagcggc ggcagctctt cgaagagctg ttcttcgagc
      961 cgtgtacggg gcgcggcgag tcttacgtgg acgagatgat ccacacgctc tgccggcacg
     1021 accgcgactt ctcggcggtg cgcgcggcct cgcgtctggt gcgcaacctc tcggtcgcgg
     1081 agatcgtcgt cgcgggcgac ctcggcgacc gcgggccgcg cctcgaccgc gtcatcgact
     1141 acctcttgca gcagccgaac gtctccttca cgtggggtaa ccacgacgtg ctgtggatgg
     1201 gcgcgtgcct cggccacgag gcgctgatgg cgaccgtgct ccgcgtctcg ctgcgttacc
     1261 ggcggctctc gcagctcgaa gagggctacg gcatcacgat gcagccgctc gaaaaactgg
     1321 cgcgcaccgt ctacgccgaa gaccccgcgg agcgcttcaa gacgaagggc gtcgggctgc
     1381 gcgacgacct gctgatggcg cggatgcaga aggccatcgc cgtcatccag ttcaagctcg
     1441 aagggcaggt cattcgccgt caccccgtgt gggagcttga gcacaggaac ctgctccacc
     1501 gcatcgaccc cgccgagggc acggtcgaga ttcaggggca gccccacccg ctccgcgatg
     1561 cgagcctgcc gaccgtcaac cccgcggacc cctacgcgct cacggaggaa gaggcgacct
     1621 gcgtcagccg cctccgccag tcgttcgtct caagctcgcg tctttggcag cacatgtctt
     1681 tcgtcgcgcg gcgcggcgcg atgtgggtgc gcagggatca cgcgctcatc ttccacggct
     1741 gcgtgcccgt ggaagaaggg ggcgccgcgc tcccgctcga agttgacggc cagacacact
     1801 cggggcgcgc gctcttcgac gcgctcgacc tcgtcgtccg ccgcgccttc cgcaaaggct
     1861 ccgaggccac ggaagtggac aaggactggc tctggtatct ctggacgggg ccgcgctcgc
     1921 cgctcttcgg caaagaccgg atggcgacct tcgagaccta cttcgtcgag gacaaggcga
     1981 cgcacaagga gaacaagaac ccttacttca agctcatcca cgaccccgcc ttctgcgcgc
     2041 gcgccgccgc cgagttcggc gtcaccgagc aggcgctcat cgtcaacggc cacgtccccg
     2101 tccgcgtcga gaagggcgaa gagccggtca agtccggcgg aggcgccgtc accatcgacg
     2161 gcgccttctc cgaagcctac ggcgaccgcg gctacacgct catcctcgcg cccgagcgcg
     2221 tcgccctcgc cgagcaccac cacttcgagt ccgtcagcga cgccatcacc gaaggcgccg
     2281 acatcgtccc gaaggtcacg accctgcgcg cctacgaccc gccccgccgc gttggtgaca
     2341 ccgaagaggg caaccccatc cgccacaccg tcgccgcgct cgaacgcctc gtcgaagcct
     2401 acgagcgcgg gctgctgctc gaagagaccg acagaccgcg ttaacccgta agtcccgccg
     2461 ttccagagat tgcggtcagc acccccttcg tcgaaaagcc cacccctaat cttgacaaaa
     2521 aagtaaggta tcgcctatga tggctgtgca tcgggaagtc tccggtcgga tttgagagct
     2581 ttatgaaggt ctcggcacag gaagagtacg ggctgcgatg tctgctgcaa ctcgcgcagt
     2641 tgggcgaggg cgagtacctg acgctcgcgc agatcgccga gcgcgagggc atctcggcgg
     2701 ccaacgcggg gaagctcttg tggattttga gcaaggcgaa tctcgtctct tcgatccgcg
     2761 ggacgaaggg cggctacagg ctcgcgcggc cctcgtcgga gattcacctg agcgagatca
     2821 tcaaggtgct ggacgaggag gacgtgctcg ccagccactg caagagctac gcgggcgtgc
     2881 tcgacacgtg cgtccacacg ggcgactgcg gcatccggcc cgtcatcgtc ggcctccacg
     2941 agatcgtgcg caacgcgctg tcgggcatca cgctcgcgca gctcatcggc accgaggcga
     3001 aggtcgatca gacgatccac cgcatacagg gtttgcgccc gcgcgtggaa accgccgagg
     3061 ggcgcgtgca gtaacggttt tcgattcgcg gaaacgctaa gactaacttc gcggggtttg
     3121 ggaaaagaga gatgagttca atcgaagttt taaccaacaa ggaatacaag tacggcttcg
     3181 tcaccgacat cgagtcggac atgatcccgc ggggtctgac cgaggacacc gtccgtctga
     3241 tttcggagaa gaagggcgag ccggagtgga tgcttgagtt ccgtctcaag gcttaccgcc
     3301 agtggctca
//
LOCUS       DBNGOH010000850         6128 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_127401, whole genome shotgun sequence.
ACCESSION   DBNGOH010000850 DBNGOH010000000
VERSION     DBNGOH010000850.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6128)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6128)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6128
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_127401"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /locus_tag="AC159P_16430"
     CDS             complement(<1..296)
                     /locus_tag="AC159P_16430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020715293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR3697082.1"
                     /translation="MDVLSEVLRVVRLEGALFFNAEFSAPWCVSEPSSNDLAPYLPAA
                     AGHLILYHFLTEGRAHVRLPDGRREELSAGDILILPHGDAHLLGNGSPVRPVDSV"
     gene            441..983
                     /locus_tag="AC159P_16435"
     CDS             441..983
                     /locus_tag="AC159P_16435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020715294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HIR3697083.1"
                     /translation="MSAVEKETKTTTKDKVGASASRRAESLADRIEEGAARLADFAEG
                     LSEEEWRTPMSNSDRRPVGVIVHHVASMYPIEIDAARAIASGEAVTDVTWEVVAELNA
                     KHAREQAGVTKDAALELLRRNSREVADGVRAFTDEELDRAAPFSLSYGAPVTAQFVIE
                     DHALRHSWHHLARIRTASGR"
     gene            2554..2760
                     /locus_tag="AC159P_16440"
     CDS             2554..2760
                     /locus_tag="AC159P_16440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697084.1"
                     /translation="MYLNDRRGEKYGRLKVTGLDDRGGQSRWYACLCECGEARSVIEW
                     KLLKGVVTERVRCEVKGKTEGAEP"
     gene            complement(3279..3935)
                     /locus_tag="AC159P_16445"
     CDS             complement(3279..3935)
                     /locus_tag="AC159P_16445"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HIR3697085.1"
                     /translation="MPRRLIFSLLLFLAVGICSAATARADIAFLVGNIPQTDENVLLN
                     TGITGNPIFGTTNQTGLAVRFASNELLTAPASGQARIEAASGTFTTATVSIGNAFPPT
                     GSFTSLILNLDATVNGTVDFTVTETDGTVHNFNNVALSGSGSNFFTIVASAGQRIQSV
                     TFTSDSPLEFMDAAQFRIGGASPTQQPIPEPTTMLLLGTGLVGIAARMRKLRKASQAD
                     "
     gene            complement(4496..4912)
                     /locus_tag="AC159P_16450"
     CDS             complement(4496..4912)
                     /locus_tag="AC159P_16450"
                     /inference="COORDINATES: protein motif:HMM:NF050000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0032196 - transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HIR3697086.1"
                     /translation="MAEDPANPHCPRCGGASWKLGFNTQGRQRYVCRACRRTFSGATV
                     RLLKRRRAREERRRLSRLSERLRRARTLAEFVGVIDARVGRAIARSPALGGLVRRGEY
                     ARRKGVSREYVRRLVKEDRLLTARVCGVEWVSEREP"
     gene            5183..5524
                     /locus_tag="AC159P_16455"
     CDS             5183..5524
                     /locus_tag="AC159P_16455"
                     /inference="COORDINATES: protein motif:HMM:NF012798.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HIR3697087.1"
                     /translation="MRRRGKGRGGDALVTPPLFQPHTNYRTLDFDKPLSTRMVQKIVK
                     RWADYSRLGDLSPHDLRRTVITRALETGLTYRQVQMMSKHRDPKTVMRYDHGRENPDQ
                     NAVIFLGYDEE"
     gene            complement(5663..>6128)
                     /locus_tag="AC159P_16460"
     CDS             complement(5663..>6128)
                     /locus_tag="AC159P_16460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TolB family protein"
                     /protein_id="HIR3697088.1"
                     /translation="KGSGRKGNKEIYLIDKDGGRLRRISNAEGNDTMPAISPDGKWVV
                     YATDRTGKGMKLWLQSLADPDDEGRLLEPGRAGLTGLDRHPRFSPDGRWVVFTSDRAG
                     WMDEWFLSGRFPQPYGELYALPVDGSGPAVRLTDDKWEDSLAFWGGPARAGR"
BASE COUNT         1149 a         1923 c         2031 g         1025 t
ORIGIN      
        1 acggagtcca ccggccttac cggggacccg ttgcccaaca ggtgggcgtc gccgtgcgga
       61 agaatcagga tgtcgcctgc actcaactct tcccgccgcc catccgggag cctgacatgg
      121 gcgcgcccct cagttaagaa gtgataaagg atcagatggc ccgcagccgc gggaagatag
      181 ggggccaggt cgtttgaact gggctcgctg acacaccagg gggcggagaa ctccgcgttg
      241 aagaagagcg ccccctcgag cctgacgact cttaagactt cggaaagcac atccattcga
      301 atagcctgag tgaaccgggt gcctgtgcca ggaatacggg acgatccgtc aagtattcaa
      361 gagcctgccc gacgtatatt ggaacaggac cgcgaaatgt gaagtaaacg aacaactcca
      421 ggaggaggag acgaaccgaa atgagtgctg ttgagaaaga gacaaagacg accactaagg
      481 acaaagtcgg agcctccgca agtcgtcgcg cagaatcact ggccgaccgc atagaggaag
      541 gcgcggcccg cctggccgat tttgccgaag ggctttcgga agaggagtgg cgtacgccga
      601 tgtcgaatag tgaccgccgc cccgtcggcg tcatagtgca ccacgttgcg agcatgtatc
      661 cgatcgagat cgatgccgcg cgagcaatcg ccagcggtga ggccgttacc gacgtcacgt
      721 gggaagtcgt cgccgagttg aacgccaaac atgcccggga gcaggcgggg gtcacgaagg
      781 acgcggcact cgaactcctg cgccggaaca gccgcgaggt cgccgatggg gtccgcgcgt
      841 tcacggatga ggagttagac cgggcagccc ccttctcttt gagctacggg gcgcctgtta
      901 cagcgcagtt cgtcattgag gaccacgcac tgcggcatag ctggcaccac ctagcgcgca
      961 tccggacggc ctcaggccga tgacatcagc caccgggtag cgctcgtcac taacgacgaa
     1021 gggtagaggc gatcttcagg gtcgccccta tccctttacg gtcgtcctgc taatattggg
     1081 acagccgccg gttgttattc cggactggcc cacttccttc gaggggccag ttgcggcccg
     1141 gtccatcaaa cgacgtgagt gcctgaagga agaaagtagg gaatccaata acgcaactgc
     1201 ggttttcaaa aaatcatggc tttcccacac gctatgcccc ctcgtacgac cagacgaagc
     1261 tgtaggcgca acgtctacga tagaaccgtg gtcggcatga acgccaaaag aaagctggaa
     1321 agtgtcgcag ttatgaagcc catcgtgaag tgctgtcccc ggataagtgc ccggctgccg
     1381 ccgagtcgtt gatgctgggg gagcggggct ggctgccaag tgttacatcg aggcggccag
     1441 cataggatga gctttactct cgatatcccc tttcatagaa catttgatat tgcttgcgaa
     1501 gtcggcaggc cctgaaattc atttgcgttg agaaacctgc tttcaacttt gaagtgcaac
     1561 acgcaaccgt ttgtaaacgc aggtcttgag ctcctcgctg ataccatgat ctgcgacatg
     1621 cagaagcagt caggacaggg acggtagtta gcccccgtac ttcatcgctc ttaccccgtg
     1681 ttgtacatac ttgtttaatc cgcgatcctt aacaggtcca cccgaccatc ctttacaacg
     1741 ccgtcgccga ggccatgtgc acggtcagct ccggcccggt cgcggacacg gcggagccga
     1801 tggcgctcca gttgcgcgcc tacgacggcg agaggatgac ggggcgggcg cggcggtgag
     1861 gcgtgcgcgg tgagcttaaa gtgacgttgc gcgaagccct gcccgaccgc cacgcgaacc
     1921 gccgcgcccg cccggcgtag ctcctgccgc ccgtgcgcga gtcgaggcgc gactggcggt
     1981 tagaaaccgg aggtctgatt cggtgactcg aagcgcttga tgggtgctca tttgctttac
     2041 taaccagtgt tatagaagtg tccgcaaagg agacggacgt ggatacatcc ggaatgggcc
     2101 tggtcccggc ggggcaagga aaaaggcctt acagtcctca gtccctgcaa ggcccgtcac
     2161 tcggagaaag ggcgcatcac cgtaacctac accggcaatg cgcgggcgcc tcgcgtccag
     2221 cgaaggcacc gattggccac gtactttagc accggtggag gcgcggcggg cgaacatttt
     2281 tcgttgcaac ttctgttgca atccgcgccg ctgaagctgc gcgcccgccc gtgacattcg
     2341 tactacgcgg aactattcct ttgtccgatg cgtggccgcc ccctcctcgc gtagattgtc
     2401 gggcgggggt tgtgggtgtg ctacgacgcc cgcaactaag ggagggggcg gctgtcttga
     2461 acctgccgcc ccttctttat tctcccgccg ctatagccac acgccggcct gcttcacgat
     2521 ccccaggcgc ctcgagctgc tacactcccc ctcgtgtacc tcaacgacag gcgcggcgag
     2581 aagtacggca ggctcaaagt cacgggcctc gacgaccgcg gcgggcagag ccggtggtac
     2641 gcctgcctgt gcgagtgcgg ggaggcgagg tcggtgatcg agtggaagct cctgaagggc
     2701 gtcgtcaccg agcgcgtgcg gtgcgaggtg aaggggaaga cggagggggc cgagccgtga
     2761 agctcggccc ccttcctacg tcacgaactt taggagggcc ggcggcatct aacaagcatt
     2821 cctcctgtag acttgaggcg ccggggcggg agaagccccc gacgcctttt ttgcttcgct
     2881 gccggccagg gcgccgcgta gtgtggcaga aggtgacgcg cccgtgaagc agattttaag
     2941 gatgcaagag tgtttgcatg gggaaggcgt gaagccacga gcccccggtt gaacgatcaa
     3001 cctcacgtcc gattcgtcac atggcttgta ggaaaatgga ttgtggggca tttacgcgcg
     3061 aagtttatgg ggcgagaggg gagagggcgc gcggggagta aagccgcgcg ccgccctcgc
     3121 gcacccacgc ccgccgcgca cgccatgccc cctttcgacg tgcgcgccgc acggcgcggg
     3181 acgcacccgg acgcggcccc atagcagcgg cccccgcacg cccggaaagc acgcgggggc
     3241 cgctgctatg tgccctgcct gggccggtcc ggacgtgctc agtccgcttg agaggctttg
     3301 cgcagcttgc gcattctggc ggcgatgcct accaggcccg tgcctaagag cagcatcgtc
     3361 gtcggctccg ggatcggctg ctgcgtcggc gacgccccgc cgatgcggaa ctgcgccgcg
     3421 tccatgaact ccagcggtga gtccgaggtg aacgtcacgc tctgaatcct ctggccagcc
     3481 gacgcgacga tggtgaagaa gttcgagccg ctcccggaga gggccacgtt gttgaagttg
     3541 tggacggtgc cgtccgtctc cgtcaccgtg aagtcgacgg tgccgttgac ggtcgcgtcc
     3601 aagttcagga tcagcgaggt gaacgagccg gtcggcggga aggcgttgcc gatgctcacg
     3661 gtggccgtgg tgaaggtgcc ggaggcggct tcgatcctgg cctgcccgct ggccggtgcc
     3721 gtcagcagtt cgttggacgc gaagcggacc gccagcccgg tctggttggt ggtgccgaag
     3781 atcgggttgc cggtgatgcc ggtgttgagc agcacgttct cgtccgtctg cgggatgttg
     3841 ccgacgagga acgcgatgtc ggcgcgggcc gtggcggcgg agcagatgcc gacggccagg
     3901 aagagcagca atgagaagat cagtctacgg ggcatatccc tatactcctt gatctggcag
     3961 agacactcgg gcgaggaccg cacgcggggc tcggcgaatg gccacggagg acgcacgggg
     4021 caagccggtc ctcggggccg taagttaatg tcggggcgtg aacagtactc gcccgcgccg
     4081 actcgatcaa tacgctaggg tacacaagga taaaaaactc ccgccgttcg agcggcccgg
     4141 cacattttga gaccggggcg acttctggac gggaaagcgg ttgaaaggca accggtcgcg
     4201 aactctcggg ggtggcggac atcaaaccga catccctcat atggacttag aggcgtccgg
     4261 gcaagggcgc tccggcgccc cttttttttg cgcccggcat ccggcatcta caagccacac
     4321 ctttgaagcc atacgcccgc ctgcgtgggg attcgtcagc agggcggccc gcggcgcggc
     4381 ataggccgat cccacagagg ccatttaagc gtgatgaatt tagaagggaa ggggcggcgg
     4441 agacaccaag acccgccgcc ctccaccacg atctctcgac cgcaggggct cgccctcacg
     4501 gctcacgttc cgagacccac tcgacgccgc agacgcgggc cgtcaacagg cggtcctcct
     4561 tgaccagccg ccggacgtac tcgcggctca cacccttgcg tcgcgcgtac tcgccgcgcc
     4621 gcaccagccc gccgagggcg ggcgagcgcg cgatggcccg cccgacccgc gcgtcgatga
     4681 ccccgacgaa ttcggcgagc gtgcgcgcgc ggcgcagcct ctccgagagg cggctcaggc
     4741 gccgccgctc ctcgcgcgcg cgccgccgct tcagaagccg cacggtcgcg ccggagaagg
     4801 tcctgcggca cgcgcggcag acgtatctct ggcggccttg cgtgttgaag ccgagcttcc
     4861 acgacgcgcc gccgcagcgc gggcagtgcg ggttagcggg gtcctccgcc attgtgcccc
     4921 ctgtctggtg gcgtcaccga acggacagtg ggcgggcgag ggggaaaaat accgcgcggc
     4981 gaccgcctgc ccgttacccc caggccgaca gggaggggct atgccacagc caacccccta
     5041 accaatcgcg ccgtagccgg ccaccgtcga gccggctctg gtgacgtcag gcgggcgcct
     5101 gcgcggggat gggatccgcg ccggactcat tccggcctgg cttgtagggg atagcgatcg
     5161 tggggcgaat gcgcgcgaag ttgtgcggcg ccggggaaag ggcaggggcg gcgatgccct
     5221 agtcacgccg cccctcttcc agccgcacac gaactaccgg acgctcgact tcgacaagcc
     5281 gctctcgacg aggatggtgc agaagatcgt gaagcggtgg gcggattact cacggctcgg
     5341 cgacctgtcg ccgcacgatc tgcgccgcac ggtcatcacg cgggcgctgg agacggggct
     5401 cacctaccgc caagtccaaa tgatgtcgaa gcatagagat ccgaagaccg tcatgcgcta
     5461 cgaccacggg cgggagaatc cagaccaaaa tgccgtcatc ttcctcgggt acgacgagga
     5521 gtaacatcac ccggagggag gctatcatgg caggctggga cggtgatgcg ctcgcggaga
     5581 gtccggagca ggaccggcgc accgatggtc tgactcgctg agttggcaga cgcgctctta
     5641 aacctattcg gtcatgtcgg tcctagcgac cggctcgggc cggcccgccc cagaaggcga
     5701 gggagtcctc ccacttgtcg tcggtcagcc ggacggccgg gccgctcccg tcgaccggca
     5761 gggcgtagag ctcgccgtag ggctggggga agcggcccga caggaaccac tcgtccatcc
     5821 agcccgcacg gtccgacgtg aagacgaccc accgcccgtc gggcgagaac cgtgggtgcc
     5881 tgtccaggcc ggtcagaccg gcccgccccg gctcgagcag gcggccctcg tcgtccgggt
     5941 ccgccagcga ctggagccac agcttcatcc ccttgcccgt gcggtccgtc gcatacacca
     6001 cccacttgcc gtcgggcgag atggctggca tcgtgtcgtt cccttcggcg ttagagatgc
     6061 ggcggagtcg gcctccgtcc ttgtctatca ggtagatctc tttgttcccc ttgcgaccac
     6121 tgcctttc
//
LOCUS       DBNGOH010000851        10272 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3793235, whole genome shotgun sequence.
ACCESSION   DBNGOH010000851 DBNGOH010000000
VERSION     DBNGOH010000851.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 10272)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10272)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10272
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3793235"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            467..1306
                     /locus_tag="AC159P_16465"
     CDS             467..1306
                     /locus_tag="AC159P_16465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease"
                     /protein_id="HIR3697089.1"
                     /translation="MKNALLTPSRRRAAAFGLVSLVCAVFVVAASANHNWRKFHWART
                     SNPFTVKLGNNLSSGWSSYLTTAASDWDNGSSVLDTDVVAGQSNSSCAPTMGRVEVCN
                     ANYGNTGWLGIASVWASGSHITQGTVKNNDYYFNQPRYDTPAWRLFVTCQEVGHTFGL
                     DHQDERFNNINLGSCMDYTNDPSGKAGTNGTLNNEHPNAHDYNQLATIYSHVDGSTTV
                     GQQNAAAKMPPAALDIIIEGPGQWGKLVRGGREQGWSDYELDFGGGHRVITHVIWASE
                     EHQ"
     gene            complement(1527..1904)
                     /locus_tag="AC159P_16470"
     CDS             complement(1527..1904)
                     /locus_tag="AC159P_16470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015231607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HIR3697090.1"
                     /translation="MDTTQIIVTLVGFGLIGWIVWYFWLWRGESVAAQAGVGGVQVVD
                     VTVRGGYQPASIVAEAGRPLRLNFTRRESTPCGEEVVLPEFGRRAHLPEDRTVPIEVT
                     PEEPGEYEFTCGMNMYRGKIIVR"
     gene            complement(1904..4294)
                     /locus_tag="AC159P_16475"
     CDS             complement(1904..4294)
                     /locus_tag="AC159P_16475"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0140358 - P-type transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HIR3697091.1"
                     /translation="MEATAANKEVFVDPVCGMKVDPGREAGTSERDGVRYYFCSAGCK
                     TKFDAGSATAEARAHEGCCGGGVASAPMQLTAAREVGGAAHEHHATGHADHGTAPRAA
                     AHAEARGAGADAEAAQEQSYRTMMRKFWFAAAVGLPLLLLMFAEFVPSWGHALMPYHR
                     LIGIVSAVVTLPVLAWSGRHFFESAWNGLLNHNTNMDTLVALGTGAAWLYSTVAVLAP
                     GLFPAGTAGMYFEVAVIVIALILLGQALEVRAKSRSSAAIRKLLELQAKTARVIRGGR
                     ELDVPVEEVVVGDTVLVRPGEKVPVDGTILEGESAIDESIVTGESVPVDKKPGDHVIG
                     SSVNKTGAFTFRATKVGAETALAQIVEMVQRAQSSKPPIGRLVDKISSYFVPSVMIVA
                     ILTFLAWFNFGPEPAVNHAVVTMVAVLVIACPCALGLATPISLMVGVGKAAEHGVLIR
                     NGEALETASKLTTVVLDKTGTITKGKPELTDVHPLEGFSEEELLRLAAVADRRSEHPL
                     AEAIVKGARERGVEVGEPDQFNAVPGHGVEAVVEGKRVAVGNRKLMARESVPSGEFEG
                     LAARLADEGKTPMFVAVDGRPAGLIGVADTLKEDSVAAVRALQRMGLEVVMITGDNER
                     TAKAIARQAGITRVLADVLPEEKARQVEKLQAEGLSEKERGGRGARLVGMVGDGINDA
                     PALAQADVGFAIGTGTDVAIEAADVTLVGGSLRGVVTAIEVSRETLRNIKQNLFGAFV
                     YNVLGIPVAAGVLFPFLGMLLSPIIAGAAMAASSITVVTNANRLRLFAPRPVEEVK"
     gene            complement(4305..4820)
                     /locus_tag="AC159P_16480"
     CDS             complement(4305..4820)
                     /locus_tag="AC159P_16480"
                     /inference="COORDINATES: protein motif:HMM:NF024803.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-responsive transcriptional
                     regulator"
                     /protein_id="HIR3697092.1"
                     /translation="MNETKRGMRVVGEEEGRRMLKIGEVSRRSGIGVEALRFYEKGGL
                     LDRPSRTFSGYRVYGEEVLERLAFIKRAQALGFSLDEIRRIVEDARKGESPCDEVREI
                     VRRRMAELDERLRELHRYRRELESTLEEWDEVGRAPGHICGLIEGSEVEHAAHDKTEG
                     LQRARGKARRA"
     gene            complement(5078..5377)
                     /locus_tag="AC159P_16485"
     CDS             complement(5078..5377)
                     /locus_tag="AC159P_16485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697093.1"
                     /translation="MKKYLKLSLLLAAAALLAGCSAGENTNSSANRGNVSNAAANANT
                     ASVNAANASNTGSATTNTGGANGNVHANMNASEHANMNANKKATPQPTGHANHNR"
     gene            complement(5447..6124)
                     /locus_tag="AC159P_16490"
     CDS             complement(5447..6124)
                     /locus_tag="AC159P_16490"
                     /inference="COORDINATES: protein motif:HMM:NF015658.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HIR3697094.1"
                     /translation="MTFAAALCALTAEVSVSAQEHTGHTSQQAGDMPPGLHFIDMMML
                     HHQQGIEMARLAEGKAQSAGVMAFAAKTSAAQEKHVQQLQWHRDNHYAGRPRMDHQQM
                     TAHMASMPGHAGMKMDMEGDMAKLRAAAGREFDRLFLDTMSHHHQMAVDMSKDAAKNA
                     EHADIRELARKAAQSQQGEIAEMSRLKASPGGTVVRKTAAKPARKPVAKKPAAKKPAA
                     QGHTGHH"
     gene            complement(6244..7764)
                     /locus_tag="AC159P_16495"
     CDS             complement(6244..7764)
                     /locus_tag="AC159P_16495"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014785014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase family protein"
                     /protein_id="HIR3697095.1"
                     /translation="MDKSRRNFLRRASVLGAAGAVAAGSTDARAQHEGHTAPQPQAPP
                     KTDNRSPASAGPPVPVQVPDVPRLPWKMEGGVKVFHLVPEVVKWQLIPGKEIIGWGYN
                     GSVPGPTIEINEGDRVRIHVTNRLPEGTSMHWHGLEVPPSQDGVPFLNQPLIEPGQTF
                     TYEFTINQNGTFFYHSHMAMQEMMGMIGFFIVHPKRSYAPRVDKDFGLILQEWALLPN
                     NPIPNSLAMEFNWLTINGRSGPHSTPMIVRQGERVRIRMVNLGMDHHPMHIHGNQFYV
                     TGTEGGRIPETAWYPGNTVLVGVAQARDVEFDAVNPGDWMLHCHLPHHMMNQMVSMVG
                     PMGHAGHGSQTGKGMEEGMGVVTQGHALSEDLGPGMGRGMGMTSQERNVSNAVGPQDQ
                     HQGHGQQPSPQQGKGTEVQPGQSERLGQISNPDDTARRKRVPGYPQDMVMFMDKEVAK
                     PETYGLPPGWTGSFMGMMTMVRVLPPDVYDEVMRRVREGIVEAPQGQPAGQQHKHE"
     gene            complement(7814..9331)
                     /locus_tag="AC159P_16500"
     CDS             complement(7814..9331)
                     /locus_tag="AC159P_16500"
                     /inference="COORDINATES: protein motif:HMM:NF014385.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HIR3697096.1"
                     /translation="MQQPAPTPPPAATPDPHAGHVMQPTPAPPQPAAPGQQHQGHQTM
                     APRPTPAPTVDHSTHQPAAPQGPVRRRAREGLIPEGPVVTLEVLERMAAERNPTLAQA
                     QASIRAAEGRRRQAGAFPNPVIGYFGEELAFRALSDNSEHGVFVEQTIPLGGKLGKAK
                     RVFEREREQAEALAEAQRFRVVNSVRALYFEALGAQQLVELRADLADLAREAVEITKE
                     LYNVGQADRPDQLEIEIEAERAEIEVLRARTDWEQAWRALGAMVNNPELRPARLAGSL
                     DEGGAVLDQEQLLTTLLRDSPELRAARAGVERARAEISRQKAERIPDLFVNGGVGYNN
                     EILERDGRKTGAEGRIEIGVNVPIFNRNRGGIAAAEAELAIAERELDRLQLSLRTRMA
                     SSFREYRNAQQMVERYRTQVVPRARQAYEMYLSNFRQMAAAYPQVLIAQRTLFQVEVE
                     YARSLVELRRNATSLRGFLLGGGLDAIGRPGEGGERTEGFRLRSASDASGDSDDR"
     gene            complement(9763..>10272)
                     /locus_tag="AC159P_16505"
     CDS             complement(9763..>10272)
                     /locus_tag="AC159P_16505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697097.1"
                     /translation="REITLFKKLSSFVFAVAFLASGWSAVMAGALCPHDECMGRPAAV
                     EHHGGHEVEVSADEGCSKGVELAGHESHEPAGEAITGQHSGHLFRGTGGHSGSCIHCV
                     GAPSTPVRSGFVGEPSQARRDAARQAEQPSVSEVVPSVAAFPALRPTQGSPPSTSQRR
                     HLLINVFLI"
BASE COUNT         1521 a         3612 c         3275 g         1864 t
ORIGIN      
        1 ctgtcgacca gtcttgcggt tggaagctgc gctattcgcg gtcgggtgca ggccgttgtc
       61 aggcgctgct gcggacacca ctaccatcag caagccttcc tcccttgtgc ccgcgagtga
      121 tcccggccgc cgcccccccc cgccgaccac gacaggcagc ttttcgtttc acaattccgg
      181 atctttgcct tgattacgca tgtcaaaagg tatattttgc ttcctcgccc tggtcgccag
      241 cgacgtttgg gtcccgttct cgcacgccgg aaggattagc ggagacagat cagcatcaac
      301 aataccgact tgttggccga tcacagcgcc accttccgat agatcgccgc ctttagctct
      361 agcgtgagag ccgtacgtta ctccttcggg cactattccc cgtcaacgga ggtccctcgc
      421 gtgggccgac ttactttgac ccatacctaa caacttcgga ggaaacatga agaacgcttt
      481 gctcacaccg tcccgccggc gcgcagccgc cttcggcctc gtgtccctcg tatgtgccgt
      541 gttcgtcgtc gccgcgtccg cgaaccataa ctggcgcaag ttccactggg cgcgcacgag
      601 caaccccttc accgtcaagc tgggcaacaa cctcagcagc ggctggtcct cctacctgac
      661 gaccgccgcg agcgactggg ataacggatc cagtgtgctg gacacggacg tcgtggccgg
      721 ccagtccaat tccagctgcg cgccgaccat gggccgcgtc gaggtctgca acgccaacta
      781 cggcaacacc ggctggctcg gcatcgcctc ggtctgggcc agcggctcgc acatcacgca
      841 gggcacggtc aagaacaacg actactactt caaccagccg aggtacgaca cccccgcgtg
      901 gcggctgttc gtgacctgcc aggaggtcgg gcacaccttc ggcctcgacc accaggacga
      961 gaggttcaac aacatcaacc tcggctcgtg catggactac acgaacgacc cgtcggggaa
     1021 ggcgggcacc aacggcacgc tcaacaacga gcacccgaac gcgcacgact acaaccaact
     1081 cgccaccatc tacagccacg tcgacggctc gacgaccgtc ggccagcaga acgcggcggc
     1141 caagatgccg cccgcggcgc tcgacatcat catcgagggg ccgggccagt ggggcaagct
     1201 cgtccgcggc ggcagggagc agggatggtc ggactacgag ctcgacttcg gcggagggca
     1261 cagggtgatc acccacgtca tctgggcgag cgaggagcac cagtaatcac ctccgtccgg
     1321 cggcttagcc gccggtcctg atcgcccggg ggtggtgaaa tgatgggcgg ggcacgtgcc
     1381 ccgcccatca tgcgttgggg aacgactgtt cagctcgccg accataccgt gttcgcgcgg
     1441 tcgagttgat ggtgtcgttg ccgcgatctc cgcgctgtta cttgaatggc gactacggcc
     1501 actgaccaga agcctgagac cgtccctcac cggacgatga tcttccccct gtacatgttc
     1561 atcccgcagg tgaactcgta ctcgcccggc tcttcgggcg tgacctcgat ggggaccgtc
     1621 ctgtcttcgg gcaggtgcgc ccgacggccg aactccggca gcaccacctc ctcgccgcac
     1681 ggcgtcgact cccgcctggt gaagttgagc cgtaggggcc gcccggcctc ggccacgatg
     1741 gacgcgggct ggtagcctcc cctgaccgtc acgtctacca cctgcacgcc gcccaccccc
     1801 gcctgcgccg cgaccgactc gcccctccac agccagaagt accagacgat ccagccgatg
     1861 agtccgaacc cgacgagcgt cacgatgatt tgcgtggtat ccattacttc acctcctcga
     1921 ccggcctcgg cgcgaagagc cgcagccgat tggcgttcgt caccacggtg atcgaggacg
     1981 cggccatcgc cgcgcccgcg atgatcggcg agagcagcat ccccaggaag gggaacaaca
     2041 cgccggccgc cacggggatg ccgagcacgt tgtaaacaaa cgccccgaac agattctgct
     2101 tgatgttcct gagcgtctcc cgcgagactt ctatcgccgt gacgacgccg cgcagactcc
     2161 cgccgacgag cgtcacgtcc gccgcctcga tcgccacatc cgtgcccgtc ccgatggcga
     2221 agcccacatc ggcctgcgcc agcgcgggcg cgtcgttgat gccgtcgccg accatcccga
     2281 cgaggcgcgc cccacgccct ccgcgctcct tctcggagag cccctcggct tggagttttt
     2341 cgacctgccg cgccttctcc tccggcagca cgtcggcgag cacgcgcgtg atcccggcct
     2401 ggcgtgcgat ggctttggcc gtgcgctcgt tgtcgccggt gatcatcacc acctcgaggc
     2461 ccattctttg tagcgcgcgg acggccgcga ccgagtcctc cttgagtgtg tcggcgactc
     2521 cgatcagccc cgcgggcctc ccgtccacgg cgacgaacat cggcgtcttg ccctcgtcgg
     2581 cgaggcgcgc ggccagtccc tcgaactcgc ccgacggaac actctcgcgc gccatcagtt
     2641 tccggttgcc gaccgccacc cgcttgccct cgacgaccgc ctccacgccg tgacctggga
     2701 cggcgttgaa ctggtcaggc tcgccgactt cgacccccct ctcgcgcgcg cctttgacga
     2761 tggcctcggc cagcgggtgc tcgctccggc ggtcggcgac cgcggcaagg cgcagcagct
     2821 cctcctccga gaagccttcg agggggtgca cgtccgtgag ctcgggttta cccttggtga
     2881 tcgtgccggt cttgtcgagc acgacggtcg tgagcttcga ggccgtctcc agcgcctcgc
     2941 cgttgcggat gagcacgccg tgctcggcgg ccttgcccac cccgaccatc agactgatgg
     3001 gcgtcgccag ccccagcgcg cacgggcagg cgatgacgag cacggcgacc atcgtcacca
     3061 ccgcgtggtt gaccgccggc tccggcccga agttgaacca cgcgaggaac gtcaggatcg
     3121 cgacgatcat caccgacggc acgaagtacg aggagatctt gtccacgagc cgcccgatgg
     3181 ggggcttcga gctctgcgcc cgctgcacca tctccacgat ctgcgccagc gccgtttcgg
     3241 cgcccacctt cgtcgcgcgg aacgtgaagg cgcccgtctt gttgacgctt gacccgatga
     3301 cgtgatcacc cggcttctta tccaccggga cggattctcc cgtcacgatg gattcgtcta
     3361 tcgccgactc gccttcgagg atagtgccgt cgacgggcac cttctcgccg ggccggacga
     3421 gcaccgtgtc gccgacgacc acctcctcga cgggcacgtc cagctcgcgg ccgccgcgga
     3481 tgacgcgggc cgtcttggcc tggagttcga ggagtttcct gatggcggcg ctcgaacggc
     3541 tcttggcccg cacctccagc gcctgcccga gcaggatgag cgcgatgacg atgaccgcca
     3601 cctcgaagta catcccggca gtgccggcag ggaacaaccc cggcgcgagg acggcgaccg
     3661 tcgagtagag ccacgcggca cccgtgccca aggcgaccag cgtgtccatg ttggtgttgt
     3721 ggttcaggag gccgttccac gcgctctcga agaagtggcg gcccgaccac gccagcaccg
     3781 gcagcgtcac gaccgccgag acgataccga tcagccggtg gtacggcatc agcgcgtggc
     3841 cccacgacgg cacgaactca gcgaacatca gcaggagcag cggcaggccg acggccgcgg
     3901 cgaaccagaa cttgcgcatc atcgtgcggt agctctgctc ttgcgccgcc tccgcgtccg
     3961 cgccggcgcc acgcgcttcg gcatgggccg cggcgcgtgg cgccgtaccg tgatctgcgt
     4021 ggccggtcgc gtgatgttcg tgcgcggccc cgccaacctc cctcgcggcg gtcaattgca
     4081 tcggggcgga cgccacgccc cctccgcagc agccctcgtg cgcccgcgct tcagccgtcg
     4141 cgctcccggc gtcgaacttc gtcttgcagc ccgccgagca gaagtagtag cgcaccccgt
     4201 cgcgctcgct cgtccccgcc tcccggcctg ggtccacttt catcccgcac accggatcaa
     4261 cgaaaacctc tttgtttgcc gccgtcgctt ccataaacac cccctcaggc gcgccgcgcc
     4321 ttaccccttg ccctctgaag cccttccgtc ttgtcgtgtg cggcgtgctc gacctccgag
     4381 ccctcaatca ggccgcagat gtggccgggc gcccgcccca cctcgtccca ctcttcgagc
     4441 gtcgactcca gctcccgccg gtagcggtgc agctcgcgca gccgctcgtc cagctcggcc
     4501 atccgccgcc ggacgatctc acgcacttcg tcgcaggggc tttcgccttt ccgtgcgtct
     4561 tcgacgatac gcctgatctc gtcgagcgag aacccgagcg cttgcgcccg cttgatgaag
     4621 gcgaggcgtt cgagcacctc ctctccgtag acgcggtaac cgctgaaagt ccgagagggc
     4681 ctgtcgagca ggccgccctt ctcgtagaag cggagcgcct cgacgccgat gccgctccgc
     4741 ctcgagacct caccgatctt cagcatccgg cggccctcct cctcgcccac aacccgcatc
     4801 ccccgtttcg tctcattcat cgtttcaagc cctccgagca ttcgatggct taagtataaa
     4861 gtctgtactc ggctacagag tcaagcggaa aagtttttac gcttcgagct tttttcctgg
     4921 ggtggcccgg ggggccgaat tgacggacgg gtgctacgcc ccgcacccgg ctacagagtc
     4981 atgctgcggg atcgaacggt gagcccgcgg ggcgtagggg ctaaaagggg taaacgttac
     5041 ctcatcccgt tgtcgcgccg gcggcgagac agccccctca ccggttgtgg ttcgcgtgcc
     5101 ccgtcggttg cggcgtcgcc ttcttgttgg cgttcatgtt ggcgtgctcg ctcgcgttca
     5161 tgttcgcgtg tacgttcccg ttggcgccgc cggtgttagt cgttgccgac cccgtgttgg
     5221 aggcgttcgc ggcattgacc gaggccgtgt tcgcgttcgc ggcggcgttg ctgacgttgc
     5281 cccggttagc gctcgagttc gtgttctccc ccgccgaaca gcccgccaag agcgcggcag
     5341 ctgcgagtaa gagcgaaagc ttcaaatact ttttcatcga aatccctcca gttgatttac
     5401 taaaaactcc gcgcccgcta atagacggca cgcgcccccg cgccgctcag tggtgacccg
     5461 tgtgcccctg cgccgccggc ttcttggcgg cgggcttctt cgccacgggc ttgcgcgccg
     5521 gcttggcggc cgtcttgcgc accaccgtcc cgccgggcga ggccttgagc cggctcatct
     5581 cggcgatctc gccctgctgc gactgcgcgg ccttgcgcgc gagttccctg atgtcggcgt
     5641 gctcggcgtt cttggcggcg tccttcgaca tgtcgacggc catctggtgg tggtggctca
     5701 tcgtgtcgag gaagaggcgg tcgaactctc gcccggcggc cgcgcgcagt ttcgccatgt
     5761 cgccttccat gtccatcttc atcccggcgt ggccgggcat gctcgccatg tgagccgtca
     5821 tctgctgatg gtccatcctg ggccggcccg cgtagtggtt gtcgcggtgc cactgcaact
     5881 gctggacgtg cttctcctgc gcggcggacg tcttcgcggc gaaagccatc acgccggcgc
     5941 tctgcgcctt accttccgcg agccgcgcca tctcgatccc ttgctggtgg tgcagcatca
     6001 tcatgtcgat gaagtgcagc cccggcggca tgtcgcccgc ctgctggctg gtgtggccgg
     6061 tgtgttcctg cgcgctgacg gaaacctccg ccgtgagcgc gcagagcgcc gccgcaaacg
     6121 tcatgattac cgcgagcgct cccgcccgtg ttcttcccca gtgctttctc attcttcctc
     6181 cctcttattc gttaaccgcg tctcggcggc gcggtgttgg tattgattag ttcggccttc
     6241 ggactactcg tgtttgtgtt gctggcccgc gggctgcccc tgcggcgcct cgacgatccc
     6301 ttcgcgcacc ctgcgcatga cctcgtcgta gacgtcgggc gggagtaccc tgaccatcgt
     6361 catcatcccc atgaaggagc cggtccagcc gggcggaagc ccgtaggtct ccggcttggc
     6421 gacctccttg tccatgaaca tgaccatgtc ctgcgggtag ccgggcacgc gcttgcgccg
     6481 cgccgtgtcg tccgggttgc tgatctgacc gagccgctcc gactgaccgg gttgcacctc
     6541 cgtgcccttg ccctgttgcg gcgacggctg ctgcccgtga ccttgatgct ggtcctgcgg
     6601 ccccaccgcg ttcgagacgt ttcgctcctg cgaagtcatc cccatcccgc ggcccatgcc
     6661 cggaccgagg tcttcgctga gcgcgtgccc ctgcgtgacg acgcccatgc cttcctccat
     6721 gcccttgccg gtctgcgagc cgtggccggc gtggcccatc ggccccacca tcgagaccat
     6781 ctggttcatc atgtgatgtg gcaagtggca gtgcagcatc cagtcaccgg ggttgacggc
     6841 gtcgaactcg acgtcgcgcg cttgggctac gccgacgagc acggtgttgc ccgggtacca
     6901 agccgtctca ggtatccgcc cgccctcggt gccggtgacg tagaactggt tgccgtggat
     6961 atgcatgggg tgatgatcca tgccgaggtt gaccatgcgg atgcggacgc gctcgccctg
     7021 gcggacgatc atcggcgtcg agtgcggccc cgaacggccg ttgatggtca gccagttgaa
     7081 ctccatcgcc agcgagttcg ggatcgggtt gttcggcagc aaggcccact cctggaggat
     7141 gaggccgaag tctttatcga cccgcggcgc gtacgaccgc ttcgggtgga cgatgaagaa
     7201 gccgatcatc cccatcatct cctgcatcgc catgtgcgag tggtagaaga aggtcccgtt
     7261 ctggttgatc gtgaactcgt aggtgaaggt ctgccccggc tcgatcaagg gctggttgag
     7321 gaacggcacg ccgtcctgcg aaggcggaac ttccagcccg tgccagtgca tggaggtgcc
     7381 ttccggcagg cggttcgtga cgtggatgcg gacgcggtcg ccctcgttga tctcgatggt
     7441 cggccccggc acgctcccgt tgtagcccca gccgatgatc tccttgccgg ggatgagctg
     7501 ccacttcacg acctctggga cgaggtggaa gaccttgacg ccgccctcca tcttccaggg
     7561 aaggcgtggc acgtcgggca cctgcacggg gacggggggg ccggcggacg ccggcgacct
     7621 gttgtcggtc ttgggcgggg cttgcggctg gggtgcagtg tgtccctcgt gctgcgcgcg
     7681 ggcgtccgtc gagccggccg ccactgcacc cgctgcgccg aggacggaag cgcgtctcag
     7741 aaaattacgt ctgctcttgt ccatctctta actcctgtcc ttgtaaaacc aatctcgtga
     7801 tacggccgcg gctttaacgg tcgtcggagt cgccggaggc gtcgctcgcc gatctcagcc
     7861 tgaagccttc ggtgcgctcg ccgccctcac cggggcgccc gatggcgtcg agtcctcctc
     7921 cgagcaggaa gccgcgcaag gatgtggcgt tccgccgcag ctcgacgagc gagcgcgcgt
     7981 attcgacctc gacctggaag agcgtgcgct gagcgatcaa cacctgcggg taggcggcgg
     8041 ccatctgccg gaagttagac aggtacatct cgtaagcctg ccgggcgcgc gggacgacct
     8101 gcgtgcggta acgctcgacc atctgctgcg cgttgcggta ctccctgaag gaggacgcca
     8161 tccgcgtgcg cagggagagt tggagacggt cgagttcccg ctcggcgatg gcgagttcgg
     8221 cctcggccgc cgcgatgccg ccgcggttgc ggttgaagat ggggacgttg acgccgatct
     8281 cgatccgccc ctcggcgccc gtcttccggc cgtcacgctc cagaatctcg ttgttgtagc
     8341 cgacgccccc gttgacgaag aggtcgggga tgcgctctgc cttctggcga gagatctcgg
     8401 cgcgtgcgcg ctcgacgccg gcgcgcgccg ccctgagctc ggggctgtca cgtagaaggg
     8461 tcgtgagcaa ctgctcctgg tcgagcaccg cgccgccctc gtcgaggctt ccggccaggc
     8521 gcgcgggccg tagttccggg ttgttcacca tcgcgccgag cgcgcgccac gcctgctccc
     8581 agtcggtgcg cgcgcggagc acctcgatct cggcgcgctc ggcctcgatt tcaatttcta
     8641 attggtcggg gcggtcggcc tgcccgacgt tgtacagctc cttcgtaatc tcgaccgcct
     8701 cgcgcgcgag gtccgcgagg tcggcgcgca gctcgaccag ttgctgcgcg ccgagggcct
     8761 cgaagtagag cgcgcggacg gagttgacca cgcggaagcg ctgcgcctcg gcgagggcct
     8821 cggcctgttc gcgctcgcgc tcgaagacgc gtttggcctt gccgagcttc ccgccgagcg
     8881 gaatcgtctg ctcgacgaag acgccgtgct cgctgttgtc gctcaaagcg cggaaggcca
     8941 gctcctcgcc gaagtatccg atcacaggat tagggaaggc gccggcctgc cgccgccgcc
     9001 cttcggcggc gcggatggac gcctgggcct gcgcgagggt cgggttcctc tcggccgcca
     9061 tcctctccaa cacttccagc gtcacgaccg ggccttccgg gatgagcccc tcgcgcgcgc
     9121 gccgcctcac cggcccctgc ggcgcggcgg gctggtgcgt gctgtggtcc accgtcggcg
     9181 cgggcgtggg ccgcggcgcc atcgtctggt ggccctgatg ttgttgaccg ggcgccgcgg
     9241 gctgcggcgg cgccggagtc ggctgcataa cgtggcccgc gtgcgggtcg ggcgtcgccg
     9301 ccggcggcgg ggtgggcgcg ggctgctgca tgtcatggcc ggcgtgcggg tccgcgggcc
     9361 gtgtctcgcg ggccggactc gccgtcggcc gggcggtcct gcgcggcgcg cgccggggcg
     9421 tagtcctgcg cgcgggagcc ttcctctgtg cctgcctccg cgcggtcctt ttcgctttcg
     9481 gcttgggcgg cgcgggcatg gtgtgcccct cgtgctgcgc atacacgggc gtttgagcgc
     9541 cgatcagaat aaacattaga actgccgcca acatccgcgc gcgcggggcg acttttacgg
     9601 tattcgagtt actcatgggc tcccccaagt ggatctgaag cactacgtgc gcgggtaaag
     9661 acacgcgcac gggtgtccgt ttactcgccg gattaaactt tgggattaaa aaggaaagcg
     9721 tgcttcaggc cggagacgca ctgaaacact tccgcgcggg agctaaatga ggaagacgtt
     9781 gatgaggagg tgcctgcgct gcgacgtact gggcggagag ccttgcgtgg gtcgcagggc
     9841 cgggaaggcc gcgaccgagg gtacgacctc actcaccgag ggctgctcgg cctgccgggc
     9901 cgcatcgcgg cgtgcctgag acggctcgcc cacgaaaccg cttctgaccg gggtcgacgg
     9961 ggcgcccacg cagtggatgc acgacccgct gtgaccgccc gtcccacgga acaggtgtcc
    10021 ggaatgctgc ccggtgattg cctcacccgc cggctcgtga gactcgtgcc cggcaagctc
    10081 cacgccctta gagcaaccct catccgccga cacttcgacc tcgtgcccgc cgtggtgttc
    10141 aaccgccgcg ggcctgccca tacactcatc atgggggcac agtgcgcccg ccatgacggc
    10201 gctccagccg ctcgccagga aggcgacggc gaagacaaaa ctggacagct tcttaaacaa
    10261 ggtgatttcg cg
//
LOCUS       DBNGOH010000852         3007 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4526539, whole genome shotgun sequence.
ACCESSION   DBNGOH010000852 DBNGOH010000000
VERSION     DBNGOH010000852.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3007)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3007)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3007
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4526539"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1721
                     /locus_tag="AC159P_16510"
     CDS             <1..1721
                     /locus_tag="AC159P_16510"
                     /inference="COORDINATES: protein motif:HMM:NF047558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TPR end-of-group domain-containing protein"
                     /protein_id="HIR3697098.1"
                     /translation="GLASRGPLRLALAVRCGLCGAQGADAVSAQESLLRAPTFAETLT
                     RAEAATAAKQWAEAAALWEKVVEANPVRGGFWDRLAWARYNAKDYPKAAAAFEKVYQL
                     GEGRPHNAAYNVACSLALAGKKEEALGWLEKSFAEGWRDLAHAEADTDLKSLHDDPRF
                     RKLLGAVDTSRMTREEGLRHDLSFLARELKRKHYAPFRKVSRDEFEAEWKRLHDDVPR
                     LSDTQFAVRLMQLMRLVGDGHTGLLPPTSRPELVKAVPVQFFYFPEGLHVVAAAPAHA
                     DLVGARVLRFNNHPVEKVTEALYTIVARDNELWPKMLGTHFMRLPAILNGLGLGTDTE
                     KLTLTVRGQDGREQTATLAAGLEFARPIRSYAPEGWVTVPQRAGGPLPLYLKNTTAPY
                     WFEHLPETKTVYFQYNSVRNAEGETLEKFAERLFKFVEEQDVDKLVLDMRWNNGGNTY
                     LSMPLLHGLIRSRKLSRRGHLFVVIGRGTFSAAQNTATFIERHTPAIFVGEPTGSSPN
                     FVGEDNLTLLPYSKLPVSISDLYWQTSWPTDRRPWIAPLLYAPPTFDLYRANRDPAME
                     AILAYK"
     gene            1887..>3007
                     /locus_tag="AC159P_16515"
     CDS             1887..>3007
                     /locus_tag="AC159P_16515"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HIR3697099.1"
                     /translation="MKFTALVALLVTNLLVAPLPCAAQHAAARKAAPAPNGVEARIRR
                     VEQGLLSPVLVKGVPAWSIEERMRHYKVPAVSVAVVNDFKVEWAKAYGVADAETKEPA
                     TTKTLFQAGSISKSVAATVALNKVEKGKLSLDEDINNKLTSWKLPDNEFTAKKKVTLA
                     NLLSHTAGLTVHGFPGYAEGAKIPTIQQVLDGARPANTAPVRVDLEPGTRFRYSGGGT
                     TIAQLAITDIERKPFPEIARETVLKPFGMTDSTYEQPLSPDWRKRAAAGHKPDGSVVK
                     GKIHVYPEMAAAGLWTTPTDLAKFAIETQLSLAGKSNKALSKEMTTRMVTPYLSDNVG
                     LGFFVEKKGSSIYFGHNGADEGFRALLLVHRDKGYGAAI"
BASE COUNT          553 a         1101 c          910 g          443 t
ORIGIN      
        1 gcggcctcgc gtcgcgcggg ccgctgcggc tcgcgctcgc cgtcaggtgc gggctgtgcg
       61 gggcgcaggg cgccgacgcc gtctcggcgc aggagtctct cttgcgcgcg cccacgttcg
      121 ccgagacgct gacgcgcgcc gaggccgcga ccgccgccaa acagtgggcc gaggccgccg
      181 cgctctggga gaaggtggtc gaggcgaacc ccgtgcgcgg cggcttctgg gaccgcctcg
      241 cgtgggcgcg ctacaacgcg aaggactacc cgaaggccgc cgccgccttc gagaaggtct
      301 accaactcgg tgagggccga ccgcacaacg ccgcctacaa cgtcgcctgc tcgctcgccc
      361 tcgcgggcaa gaaggaggag gctctcggct ggctcgaaaa gtctttcgcc gaagggtggc
      421 gcgacctcgc gcacgcagag gccgacacgg acttgaagtc gctccacgac gacccgcgct
      481 tccggaaact cttgggcgcc gttgacacga gcaggatgac gcgcgaagag ggcttgcgcc
      541 acgacctctc gttcctcgcg cgcgagctga agcggaagca ctacgccccg ttccgcaaag
      601 tgtcgcgcga cgagttcgag gccgaatgga agcggctcca cgacgacgtg ccgcggctct
      661 ccgacacgca gttcgccgtc cgcctgatgc agctcatgcg gctcgtcggc gacggccaca
      721 cgggcttgct gcccccgacc tcgcggcccg aactcgtcaa ggccgtcccc gtccagttct
      781 tctacttccc cgaaggtctc cacgtcgtcg ccgccgcgcc cgcgcacgcc gatctcgtcg
      841 gcgcgcgagt cttgcgcttc aacaaccacc ccgtcgagaa agtgaccgag gcgctctaca
      901 ccatcgtcgc ccgcgacaac gaactctggc cgaagatgct cggcacgcac ttcatgcgcc
      961 tgcccgcgat cctcaacggc ctcggcctcg gcaccgacac ggagaagctg accctcaccg
     1021 tccgcggcca ggacggcagg gagcagaccg ccacgctcgc cgcgggcctt gagttcgcgc
     1081 ggcccatccg cagctacgcg cccgagggct gggtcactgt cccgcaacgc gcgggcgggc
     1141 cgctcccgct ctatctcaag aacacgaccg ccccctactg gttcgagcac ctgcccgaga
     1201 cgaagacggt ctacttccag tacaacagcg tgcgcaacgc ggagggcgag acgctggaga
     1261 agttcgccga gcggctcttc aagttcgtcg aagagcagga cgtggacaaa ctcgtcctcg
     1321 acatgcgctg gaacaacggc ggcaacacct atctgagcat gccgctgctg cacgggctga
     1381 tccgcagccg caagctcagc cgccgcggcc acctcttcgt cgtcatcggg cgcggcacct
     1441 tctccgccgc gcagaacacc gcgaccttca tcgagcgcca caccccggcc atcttcgtcg
     1501 gcgagccgac cggctccagc cccaacttcg tcggcgaaga caacctgacg ctgctcccct
     1561 acagcaagct cccggtcagc atctccgacc tctactggca gacctcctgg ccgacagacc
     1621 gccgcccctg gatcgcgccg ctgctctacg cgccgccgac cttcgacctc tatcgcgcca
     1681 accgcgaccc cgcgatggaa gccatcctcg cctacaaatg aaagtgctga gtactgagtg
     1741 ctgagtactg agtactgagt aagaacagcg tgcctctcat caacagtcaa tgcttttact
     1801 cagtactcag cactcagcac ttcccttgtc cttgatttaa agtccggctc gaacataatc
     1861 tatcgcgcgc gaaaggaact accgtcatga aattcaccgc ccttgtcgca cttctcgtca
     1921 cgaatcttct cgtcgcccct ctgccttgtg ccgcgcagca cgcggcagcc aggaaggcgg
     1981 cgcccgcgcc gaacggcgtc gaggcgcgca tccgccgcgt cgagcagggc ttgctctcgc
     2041 ccgtgctcgt caagggcgtg cccgcgtgga gcatcgagga gcggatgagg cattacaaag
     2101 tcccggcggt cagcgtcgcc gtcgtcaacg atttcaaagt cgagtgggcc aaagcctacg
     2161 gcgtcgcgga cgcggagacg aaagagcctg cgacaacgaa gacgctcttc caggccggct
     2221 cgataagtaa atccgtcgcg gcgacggtcg cgctcaacaa ggtcgagaag ggcaagctct
     2281 cgctcgacga ggacatcaac aacaagctca cctcttggaa gctgcccgac aacgaattca
     2341 ccgcgaagaa gaaggtgacg ctggccaacc tgctgagcca cacggcggga ctcaccgtcc
     2401 acggcttccc cggctacgcc gagggcgcga agataccgac gattcagcag gtgctcgacg
     2461 gcgcgcggcc cgcgaacacc gcgcccgtcc gcgttgacct tgagccgggc acgcgcttcc
     2521 gctactccgg cggcggcacc accatcgcgc agctcgccat cacggacatc gagcgcaagc
     2581 ccttccccga gatcgcgcgc gagaccgtcc tcaagccctt cggcatgacg gacagcacct
     2641 acgagcagcc gctctccccg gactggcgca agcgcgccgc cgcgggccac aagcccgacg
     2701 gctcggtcgt caagggcaag attcacgtct atccggagat ggccgccgca ggcctgtgga
     2761 cgacgccgac cgacctcgcg aagttcgcca tcgagacgca gctctcgctc gcgggcaagt
     2821 cgaacaaggc gctctcgaaa gagatgacga cgcgcatggt gacgccctac ctgtcggaca
     2881 acgtgggcct cggcttcttc gtcgagaaga agggcagctc gatctacttc ggccacaacg
     2941 gcgccgacga gggcttccgc gcgctgctcc tcgtccaccg ggacaagggc tacggcgccg
     3001 ccatcat
//
LOCUS       DBNGOH010000853         2085 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1046195, whole genome shotgun sequence.
ACCESSION   DBNGOH010000853 DBNGOH010000000
VERSION     DBNGOH010000853.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2085)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2085)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2085
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1046195"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            149..223
                     /locus_tag="AC159P_16520"
     tRNA            149..223
                     /locus_tag="AC159P_16520"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:181..183,aa:Val,seq:gac)
     gene            554..>2085
                     /gene="thrS"
                     /locus_tag="AC159P_16525"
     CDS             554..>2085
                     /gene="thrS"
                     /locus_tag="AC159P_16525"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HIR3697100.1"
                     /translation="MSEINVQLNDGRSAQVAPGASVADALKQLDRDAAKQALAAKVNG
                     REVDLSRRLEELASNGGGAEGGGPVEPMEIEPVLPGTLGGLDVIRHSTAHLLAAAVLD
                     LFPGTKLGIGPALLEDPRYGFFYDVIAPRPLTDEDLPVIEKRMRELAKRNLSYRRDVV
                     PKHEALRVFGERDEPLKCELIENKAGDQVSVYYIDGSPFMDFCLGPHVPNTNRLRAFK
                     LLSLSGAYWMGDAEREQMQRIYGTAFATQDELDAWVRQREEAERRDHRRLGRELDLFS
                     IQDAYGSGLVFWHPKGAVIRKAMEDYLYDELNRRGYTFVYTPHIAKRELWQTSGHEQN
                     YGDSMFAPTKLEETEFRLKPMNCPFHIGIFKSQQRSYRDLPQRYAEMGTVYRAELSGT
                     LHGLMRVRGFTVDDAHLFCTPEQVRKEISDCVDFVAEVFRTFGFEKVKFELSVKGSDT
                     NKQWLGSDEDWAKAEAALAEALDERGVPYERIEGEAAFYGPKIDLKIEDAIGRLWQLG
                     TV"
BASE COUNT          381 a          668 c          696 g          340 t
ORIGIN      
        1 gagcggagtg cggcgacaat tctttcgccg gtgacaaagg gcttcggcgg gcgggaaaac
       61 atcccgcccg ccgcgcttga cacgaagcga agccgcttgc taacttaacg ttccgctttt
      121 ttaaggcgcg aggtgttcgg ttatttgtag gcacgtagct caggggtaga gcactacctt
      181 gacacggtag gggtccgcgg ttcaaatccg cgcgtgccta ccattcgact tttcgacagg
      241 aagctccgga agacctttgc cggaagttgt acgaagcgat gacgcgaact cgaactacgt
      301 ttcggttcta agtcaacgac acggcccgcc cccactacga gaggcgcggc cctggcggcg
      361 agggcgcttc tgtcgaatcc gatctgttgg tcaaaatccc tgcaagggac tgcataattc
      421 ggttgacgct ttctctcgtc cgcggggccg cccgtcgctt tagcgcgggg cttcgggaac
      481 ggggccgcgc gcggttattt aaaccgcgcc gacccattcc gtttcattgc ccacggcggc
      541 ggagaataaa gcgatgagtg aaatcaacgt tcagttaaac gacgggcgtt cggcgcaggt
      601 ggcgccgggc gcgagcgtgg ccgacgcgct caagcaactc gaccgcgacg ccgccaagca
      661 ggcgctcgcg gcgaaagtca acgggcgcga ggtcgatctc tcgcgccgtc tcgaagagct
      721 ggcgtcgaac ggcggcggcg ccgaaggcgg cgggccggtc gagccaatgg agatagagcc
      781 ggtgctgccc ggcacgctcg gcggcctcga cgtgatccgg cattcgacgg cgcacctgct
      841 ggccgccgcc gtgctcgacc tctttccggg gacgaagctc ggcatcggcc cggcgcttct
      901 ggaagacccg cgctacggct tcttctacga cgtgatcgcg ccgcgcccac tcacggacga
      961 agacctgccc gtcatcgaga agcggatgcg cgagctggcc aagcgcaacc tctcgtaccg
     1021 ccgcgatgtc gtgccgaagc acgaggcgct gcgcgtcttc ggcgagcggg acgagccgct
     1081 gaagtgcgag ctgatcgaga acaaggcggg cgaccaggtc agcgtctact acatcgacgg
     1141 ctcgccgttc atggacttct gcctcggccc gcacgtgccg aacacgaacc gcctgcgcgc
     1201 cttcaagctg ctctcgctct cgggcgccta ctggatgggc gacgccgagc gagagcagat
     1261 gcagcgcatc tacggcacgg ccttcgccac gcaggacgag ctggacgcgt gggtcaggca
     1321 gcgcgaagag gcggagcggc gcgaccaccg ccgcttgggc cgtgaactcg acctcttctc
     1381 gattcaggac gcgtacggct cgggcctcgt cttctggcac ccgaagggcg cggtcatccg
     1441 caaggcgatg gaggactacc tctacgacga gctgaaccgc cgcggctaca cgttcgtcta
     1501 cacgccgcac atcgccaagc gcgagctgtg gcagacgagc gggcacgagc agaactacgg
     1561 cgactcgatg ttcgcgccga cgaagctcga agagacggag tttcggctca agccgatgaa
     1621 ctgcccgttc cacatcggca tcttcaagtc gcagcagcgc tcctaccgcg acctgccgca
     1681 gcgctacgcc gagatgggca cggtctaccg cgccgaactc tccggcacgc tccacggcct
     1741 gatgcgcgtg cgcggcttca cggtcgatga cgcgcatctg ttctgcacgc ccgagcaggt
     1801 gcgcaaagag atcagcgact gcgtggactt cgtggccgag gtcttccgca cgttcggctt
     1861 cgagaaggtg aagttcgagc tgtcggtgaa gggcagcgac acgaacaagc agtggctcgg
     1921 ctcggacgag gactgggcga aggccgaggc cgcgctcgcc gaggctctcg acgagcgcgg
     1981 cgtcccttac gagcgcatcg agggcgaggc cgcgttctac gggccgaaga tcgacttgaa
     2041 gatcgaggac gccatcggtc ggctgtggca gctcggcacg gtgca
//
LOCUS       DBNGOH010000854         2366 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4160115, whole genome shotgun sequence.
ACCESSION   DBNGOH010000854 DBNGOH010000000
VERSION     DBNGOH010000854.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2366)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2366)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2366
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4160115"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(45..1589)
                     /locus_tag="AC159P_16530"
     CDS             complement(45..1589)
                     /locus_tag="AC159P_16530"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF002074.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HIR3697101.1"
                     /translation="MEILTSGGRPADADVQALAVAVFKDEKADEGILKDLDAAAGGIV
                     RSILEAEEMKGKEGESAYVHIPADGGGLKAKRLLLVGVGAREDYKAAQVSQFAGLVAR
                     TLRSRNDKSIGLVPRLEGGDAARAAKAAVEGAIIGLFEPDKYRTVEKEERSVERLTVV
                     VEGADEELLRTGAERGRVVGESVNFTRDLANEPGAYMTPTIMAERAQQIAQEFGLEVE
                     VLDQPQLEEMGMGAFLSVARGSDQPPKLMVLKYMPDGKSAVGEGEDYLALVGKGITFD
                     TGGISLKPGENMELMKYDMTGGATVLGVMRAVGQLKPPIPLLGVVPATENMPSGKATK
                     PGDVVRAMSGKTIEVINTDAEGRLILADAVSYAKKLGATKVIDMATLTGAVTIALGDV
                     NTGIMGTDQALINEVIESGREVGEKFWQLPLDKEYSKQIKSDIADIKNVGGRKAGTIT
                     AAAFIKEFADGISWAHLDIAGTAWGDEAKPFRSKGPTGIAVRTLLNFIERASRTAEGG
                     EKGDGG"
     gene            complement(1602..2066)
                     /gene="smpB"
                     /locus_tag="AC159P_16535"
     CDS             complement(1602..2066)
                     /gene="smpB"
                     /locus_tag="AC159P_16535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007503122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HIR3697102.1"
                     /translation="MADEEKVIVTNRAAFHEYHILDKYEAGIALTGTEVKSVRAGQIQ
                     LKESYVSVRDGEAWLFNAHVSHYSHGNRLNHDPTRTRKLLLHRREIDRLASEATVKGM
                     TLVVTRVYLKKGRVKFEIGVARGKKLYDKRETEMRRTVERETRAQMKERAKE"
     gene            complement(2181..>2366)
                     /locus_tag="AC159P_16540"
     CDS             complement(2181..>2366)
                     /locus_tag="AC159P_16540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020466224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HIR3697103.1"
                     /translation="GMSEVAEGVRTTRSVKQLAARAGVEMPITDEVHAVLYEGKGVRD
                     AVESLMARPPKGEFEKQ"
BASE COUNT          331 a          825 c          728 g          482 t
ORIGIN      
        1 gtgtacacac cgccgtcctg tgttttggac actgcgcggg tcgatcagcc gccgtcgccc
       61 ttctcgcctc cttcagccgt gcgcgaggcg cgctcgatga agttgaggag cgtgcgcacc
      121 gcgatgccgg tcgggccttt cgagcggaag ggtttggcct cgtcgcccca ggcggtaccc
      181 gcgatgtcga ggtgcgccca cgagatgccg tcggcgaact ccttgatgaa ggcggcggcg
      241 gtgatggtgc cggccttgcg cccgccgacg ttcttgatgt cggcgatgtc ggacttgatc
      301 tgcttggagt actccttgtc gagcggcaac tgccagaact tctcgccgac ctcgcgcccc
      361 gactcgatga cctcgttgat gagcgcctga tcggtgccca tgatgcccgt gttcacgtcg
      421 ccgagcgcga tggtgacggc gcccgtcagc gtcgccatgt cgatgacttt ggtcgcgccg
      481 agctttttgg cgtaggagac ggcgtcggcg aggatgaggc gcccctcggc gtccgtattg
      541 atgacctcga tggtcttgcc ggacatggcg cgcaccacgt cgcccggctt ggtcgccttg
      601 cccgagggca tgttctcggt cgccgggacg acgccgagca gcgggatcgg cggcttgagc
      661 tgcccgacgg cgcgcatcac gccgagcacc gtcgcgccgc ccgtcatgtc gtacttcatc
      721 agctccatgt tctcgcccgg cttgagcgag atgccgccgg tgtcgaaggt gatgcccttg
      781 ccgacgagcg cgaggtagtc ttcgccctcg cccacggcag acttgccgtc gggcatgtat
      841 ttcaagacca tcagcttcgg cggctgatcc gagccgcgcg ccacgctgag gaaggcgccc
      901 atccccatct cttcgagctg cggctggtcg agcacttcga cctcaaggcc gaactcctgc
      961 gcgatctgct gcgcgcgctc ggccatgatg gtcggcgtca tgtaggcgcc cggctcgttc
     1021 gccagatcgc gcgtgaagtt gaccgactcg ccgacgacgc gaccacgctc ggcgcccgtg
     1081 cgcaacaact cttcgtcggc gccctcgacg acgaccgtca ggcgctcgac gctccgctcc
     1141 tccttctcga cggtgcggta cttgtccggc tcgaacaggc cgatgatggc accctcgacc
     1201 gcggccttcg cggcgcgtgc ggcgtcgccg ccttcgagac gcggcacgag gccgattgac
     1261 ttgtcgttgc gcgaacgcag cgtgcgcgcg accagtccgg cgaactgcga gacctgcgcc
     1321 gccttgtagt cttcgcgcgc cccgacgccg acgagcagca gccgcttcgc cttcagcccg
     1381 ccgccgtccg cagggatgtg gacgtaggcg ctctcgccct ccttgccctt catctcttcg
     1441 gcttcgagga tggagcggac aatgccgccc gccgccgcgt cgaggtcttt caggattccc
     1501 tcgtccgcct tctcgtcttt gaacacggcg acggccagcg cctgtacgtc cgcatcggcg
     1561 gggcgcccgc cgctggtgag aatttccata gttggtttcc tttactcttt cgcgcgctcc
     1621 ttcatctgag cgcgggtctc gcgctccacg gtgcgtcgca tctcggtttc gcgcttgtca
     1681 tataattttt taccacgagc gacgcctatt tcaaatttga cgcgcccctt tttcaagtag
     1741 acgcgcgtga cgaccagcgt catccccttc acggtcgcct ccgacgccag ccggtcgatc
     1801 tcgcgccggt ggagcaggag cttgcgcgtc cgcgtcgggt cgtggttcag gcggttgccg
     1861 tgcgagtagt gcgacacgtg cgcgttgaag agccacgcct cgccgtcgcg cacggagacg
     1921 tacgactcct tcaactgaat ctgccccgcg cgcacgctct tcacttccgt cccggtcaac
     1981 gcgatccccg cctcgtactt gtcgaggatg tggtactcgt ggaacgccgc ccgattcgta
     2041 acgatcactt tctcttcatc agccataacg aacaacagta ggcagtaggc agtaggcagt
     2101 agaagaacgt tttcctactt gtccttcagg tatcagcgtc agcatcagtc gggcggagcc
     2161 tactgcctgc tgcctgctgc ctactgcttt tcgaactccc ccttcggcgg cctcgccatc
     2221 aggctctcga cggcgtcgcg cacgcccttg ccttcgtaga ggacggcgtg cacctcgtcc
     2281 gtgatcggca tctcgacgcc tgcgcgcgcg gccaactgct tgacggagcg cgtcgtacgc
     2341 acgccctcgg ccacctcgct catgcc
//
LOCUS       DBNGOH010000855         2173 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2144114, whole genome shotgun sequence.
ACCESSION   DBNGOH010000855 DBNGOH010000000
VERSION     DBNGOH010000855.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2173)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2173)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2173
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2144114"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..484)
                     /locus_tag="AC159P_16545"
     CDS             complement(<1..484)
                     /locus_tag="AC159P_16545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697104.1"
                     /translation="MPRTPCPHKNRRLRTLVATTLFAALALASLAGLDTAHAQRGAKN
                     AKKSASTKRVFVVRGSDSGAGSRQTIKSDNPLNDYSAYRSGDRFNVVIPNADANAMGK
                     GGAGRGYTDMQVQQRGNDVVLSYKLEPGAKPRVEQKFNRLDVVFETPEGGPQTAATRH
                     A"
     gene            complement(550..>2173)
                     /locus_tag="AC159P_16550"
     CDS             complement(550..>2173)
                     /locus_tag="AC159P_16550"
                     /inference="COORDINATES: protein motif:HMM:NF025030.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HIR3697105.1"
                     /translation="AGASRKPVKDSGAVAAAKARGAERVEKQASAVVSAAPSPAFEQS
                     KGDRRRESVPVERERRNAGRGADDEASRQQKKMGAAESAANGHGERRAEARAGVPSVV
                     FGSYRIDQEVTQLVRGWPHSIEVLASRAVDDRRAVETSLLKALHAAETDEDGRRRVRT
                     ACEDYGLVARQNAMLLLSSNAYERTAAARTLAEMKSAQALPFLTEALYDADAVVRTEA
                     VKALGALGLPSSIGAMLDVARRHPDIPATILGPALTACSVETVEAGEGFREEGVTLAR
                     AASAEQFVSEMRGVEPVSVRQLPEWLEDATLQDALDRLGSADVEARIMAAQSLARFQV
                     RRAVEALAAMAVRDEAAAVRAAAVTALGSIDHESVFASVLIAMADDSREVRAAAARTM
                     SRLGFERADAYMHVTETADRPTLCEAARACVKSGLAAQAVDRLTSEDRRQAYEAFSVL
                     ALVVKGGEAEVILDAVGTHRDTEVRLAAVHVLGLMDDPGLAERLEMIARRGGLPVPVH
                     AAISEVAGRVVRSRQEVFESCTPQRQVGRNAW"
BASE COUNT          292 a          846 c          700 g          335 t
ORIGIN      
        1 gcgcatgccg ggtcgccgcc gtttgcgggc cgccttcggg ggtctcgaag accacgtcga
       61 ggcggttgaa cttctgctcg acgcgcggct tcgcgcccgg ctcgagcttg tagctgagca
      121 ccacgtcgtt gccgcgctgc tgcacctgca tgtccgtgta gccgcgcccc gcgccgccct
      181 tccccatggc gttggcgtcg gcgttgggga tgacgacgtt gaagcggtcg ccgctgcggt
      241 aggccgagta gtcgttgagc gggttgtcgg acttgatggt ctggcgcgaa cccgcgcccg
      301 agtcgctgcc gcgaacgacg aagacgcgct tcgtgctcgc cgacttcttc gcgttcttcg
      361 cgccgcgctg cgcgtgcgcg gtgtcgaggc cggcgagcga ggcgagcgcg agcgccgcga
      421 acagggtcgt ggcgacgagc gtccgcaggc gacggttttt gtgcgggcaa ggtgtacggg
      481 gcattgcggt agatgtgttc gggaacggga agggggcgga cgcattatgc aacacggctc
      541 gcccgattgt caccaggcat ttcgacccac ttgtcgttgc ggagtacaac tctcgaagac
      601 ctcctgccgc gagcgcacga cgcgcccggc cacttcgctg atcgccgcgt gcacgggcac
      661 cggcagcccc ccgcgccgcg cgatcatttc gagtcgctcg gccagtccgg ggtcgtccat
      721 caggccgagc acgtgcaccg ccgcgagcct cacctccgtg tccctgtgcg tcccgacggc
      781 gtcgagtatc acctcggcct cgccgccctt caccaccagc gcgagcacag agaaagcctc
      841 gtaagcctgc cgcctgtctt cgctcgtcag gcggtcaacc gcctgcgccg ccagccccga
      901 cttgacgcac gcgcgcgccg cctcgcagag cgtcggcctg tccgccgtct ccgtgacgtg
      961 catgtacgcg tcggcgcgct cgaacccgag ccgcgacatc gtgcgcgcgg cggccgcgcg
     1021 cacctcgcgc gagtcgtcgg ccatcgcgat caggacggac gcgaagaccg actcgtggtc
     1081 gatggagccg agcgccgtca ccgccgccgc gcgcactgcc gccgcctcgt cgcgcacggc
     1141 catcgccgcc agcgcctcga ccgcgcgccg cacctggaac ctcgccagcg actgcgccgc
     1201 catgatgcgc gcctccacgt ccgcgctccc gaggcggtcg agcgcatcct gaagcgtcgc
     1261 gtcttcgagc cactccggaa gctggcgcac gctcaccggc tcgaccccgc gcatctcgct
     1321 cacgaactgc tcggcgctcg cggcccgcgc cagcgtcacg ccctcttccc gaaagccttc
     1381 gcccgcctcc accgtctcga ccgagcaagc cgtcaacgcc ggcccgagga tcgtcgccgg
     1441 gatgtccgga tgacgacgcg ccacgtcgag catcgcgccg atggacgaag gcagacccaa
     1501 agcgccgagc gccttgacgg cctccgtgcg gacgaccgcg tccgcgtcgt agagcgcttc
     1561 ggtgaggaac ggcagagcct gcgccgactt catctcggcg agcgtgcgcg ccgccgccgt
     1621 ccgctcgtac gcatttgaag acagaagcag catcgcgttc tgccgcgcca caaggccgta
     1681 atcctcgcac gccgtgcgga cgcggcgacg accgtcttcg tccgtctccg ccgcgtgcag
     1741 cgccttcaac agagatgtct cgacagcgcg cctgtcatcg accgcgcgcg aagccagcac
     1801 ctcgatgctg tgcggccagc cgcgcacgag ctgcgtcacc tcctggtcga tgcggtacga
     1861 gccgaagacg accgaaggca cgcccgccct cgcctccgcc ctgcgctcgc cgtggccgtt
     1921 cgccgcagac tccgccgcgc ccattttttt ctgctgccgc gaagcctcgt catccgcgcc
     1981 gcgccccgcg ttccggcgtt cacgctcgac cggcacggac tctctccggc ggtcgccctt
     2041 cgactgctcg aacgcgggcg acggcgccgc cgacacgacc gcgctcgcct gcttctcgac
     2101 cctctccgcg ccccgcgcct tcgccgccgc gaccgcgccc gaatctttca cgggcttccg
     2161 cgaagcgccc gct
//
LOCUS       DBNGOH010000856         3256 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2144185, whole genome shotgun sequence.
ACCESSION   DBNGOH010000856 DBNGOH010000000
VERSION     DBNGOH010000856.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3256)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3256)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3256
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2144185"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..339
                     /locus_tag="AC159P_16555"
     CDS             <1..339
                     /locus_tag="AC159P_16555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697106.1"
                     /translation="INPARTKYGTPEDWTFESYELARRIVNVTCQWGQQAAAPPKGKP
                     RRRGRSPRAAVMQTTAQGVAGTPCVADSAARPIRLGDNYYRGGYEKELDEQLLWAGVR
                     LALLLNDIFR"
     gene            501..1991
                     /locus_tag="AC159P_16560"
     CDS             501..1991
                     /locus_tag="AC159P_16560"
                     /inference="COORDINATES: protein motif:HMM:NF025056.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HIR3697107.1"
                     /translation="MSYLPSFDYDLFISYAHVDNQPGGRRGWVHRFQEELVLRLDRRL
                     GRIGAAKIWWDSQLDGSQVFGQKIRNTINRSALFLALTSEGYLRSDYCRDELNWFCEK
                     AEAEPPTLIVGDRARVFNVLLDNIPHTRWPARYGGTNGYPFYDEEVGLPLETRRKPFD
                     LQLNRMVKVICELLEAMRGAPEEEEEEEPFTVFIADTATTLRNVRSRLCNELQGKGVR
                     IAERVPPPHPLREHEEAVAEKVKSADLCVHLLDAVPGTEIIDEPSRSYLETQAELSLA
                     HARWQFVWVPSDLTAERIEVVEDEAHRNLLRRLEAGPAAAARDAGGYKFVRDLPSNIS
                     NNILERIEQIRKERAAAQPAAAGEPLAALVATHFKDQQYVMALSSFLLQQQVIPLINR
                     EEDSPKKNLEYFESQLRKASLFIVVFGGVPPEWVRERLGEALKVAVTMQHCALRACGV
                     YLAPPRKNAADVQFNRTLFPLEILDNTERFDPRTLAPLLAKLQAGA"
     gene            1991..>3256
                     /locus_tag="AC159P_16565"
     CDS             1991..>3256
                     /locus_tag="AC159P_16565"
                     /inference="COORDINATES: protein motif:HMM:NF044287.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR3697108.1"
                     /translation="MQQSALQENPFVGLRPFNVKESLLFFGRGAQTGELLERLYTRRF
                     VSVVGRSGCGKSSLILAGLIPKLKAGFLVADRDRWVVTTMKPGDRPLYNLAAAVLEAI
                     GLDPTAADFTARTDGLVEEVGAEGVRALTARLAPVLGHRDANLLLLVDQFEEIFRFGA
                     RARDDDDDAGEQDAAAVRQRDRAADFVSLMLALSEQRDVPVYVVMTMRSDFLGDCDAF
                     RGLPEAMNHSQYLVPRLSTQQREEAISGPIRLSGAAITPRLLDLVRNDVGDKLDQLPV
                     MQHAMMRTWENWRVGDGGPVDVPDYLAAGTIRNALSDEAEDVLRAMDEEGGGQRKLTM
                     LVFQALTNTDAENRRIRRPKRLRELEAITGAGGEQIMKIVSLFEGGGRSFLTVARDHA
                     RGNHLIDISHESLIRQWKTLREWVDKEADG"
BASE COUNT          547 a         1175 c         1088 g          446 t
ORIGIN      
        1 atcaacccgg cgcggacgaa gtacgggacg cccgaagact ggactttcga gtcgtacgaa
       61 ctcgcgcgcc gaatcgttaa cgtcacctgt caatggggcc agcaggcggc ggccccgccg
      121 aaggggaagc cgaggaggcg cggcaggtcg ccgcgcgccg cggtgatgca gaccaccgcg
      181 cagggtgtcg ccgggacgcc ctgcgtggcg gacagcgccg cccggcccat ccggctcggg
      241 gacaattact accgcggcgg ctacgagaaa gaactggacg agcagctcct gtgggcggga
      301 gtccgcctcg cgctcctgct caacgacatc ttcaggtgac gcggggcgcg ccccgcccgg
      361 ccccgcgccg cgacgagcag gggcggctcc gcccgcgacc gacgactcca accgtccgac
      421 cccgtccgcc gccctgtgcg ccggacggag gcaccgcttc ccggcggccc acaaccctcc
      481 gccgtttcaa ggaaggcgct atgtcttact taccctcatt cgactacgac ctgttcatca
      541 gctacgcgca cgtggacaac cagcccggcg gcaggcgggg ctgggtgcat cgctttcagg
      601 aggagcttgt cctccggctc gaccggcggc tcgggcgcat cggcgcggcc aaaatctggt
      661 gggactcgca actggacggc agtcaggtct tcggccagaa gatccggaac acgatcaacc
      721 gctcggcgct cttcctcgcg ctgacctccg agggttacct caggtcggac tactgccggg
      781 acgagctgaa ctggttctgc gagaaggccg aggccgagcc gcccaccctc atcgtcggcg
      841 accgcgcccg cgtcttcaac gtgctgctcg acaacatccc gcacacgcgc tggcccgccc
      901 gctacggcgg gacgaacggc taccccttct acgacgagga ggtgggtctc ccgctggaga
      961 cccggcgcaa gccgttcgac ctccagctca accggatggt gaaggtcatc tgcgagctgc
     1021 tggaggcgat gcggggcgcg ccggaggagg aagaggagga ggagccgttc acggtcttca
     1081 tcgccgacac ggccaccacg ctcaggaacg tgcgctcgcg cctctgcaac gagttgcagg
     1141 ggaagggcgt ccggatcgcc gagcgcgtcc cgccaccgca cccgctgcgc gagcacgaag
     1201 aggccgtcgc cgagaaggtc aagagcgccg acctctgcgt gcacctgctc gacgccgtgc
     1261 ccggcaccga gatcatcgac gagccgagca ggtcgtacct cgaaacgcag gccgagctga
     1321 gcctcgcgca cgcgcggtgg cagttcgtct gggtgccgag cgacctgacc gccgagagga
     1381 tcgaggtggt cgaagacgag gcgcaccgca acctgctccg gcgcctcgag gccggccccg
     1441 ccgccgccgc gcgcgacgcc ggcggctaca agttcgtccg cgacctgccg agcaacatct
     1501 cgaacaacat tctggagcgg atcgagcaga tcaggaagga gcgcgcggcg gcgcagccgg
     1561 cggcggcggg cgagccgctg gccgcgctcg tggccacgca cttcaaagac cagcagtacg
     1621 tgatggcgct cagcagcttc ctcctccagc agcaggtcat cccgctcatc aaccgcgagg
     1681 aggactcgcc gaagaagaac ctcgaatact tcgagagcca actgcgcaag gccagcctct
     1741 tcatcgtcgt cttcgggggc gtgccgcccg agtgggtgcg cgagcggttg ggcgaggcgc
     1801 tgaaggtcgc cgtcaccatg cagcactgtg cgctgcgcgc ctgcggcgtc tacctcgcgc
     1861 cgccgcgcaa gaacgccgcg gacgtgcagt tcaaccgcac cctcttccca ctcgaaattc
     1921 tggacaacac cgagcgcttc gacccgcgga cgctcgcccc gctgctcgcc aaacttcagg
     1981 caggtgcgtg atgcaacaga gtgctttaca agagaaccct ttcgtcggcc tgcgcccctt
     2041 caacgtcaag gagagcctgc tcttcttcgg gcgcggggcg cagaccgggg agctgctgga
     2101 gcggctctac acgcgccgct tcgtgtcggt cgtcgggcgt tcgggctgcg gcaaatcgtc
     2161 gctcatcctc gctgggctga tcccgaaact gaaggccggc ttcctcgtcg ccgaccgcga
     2221 ccgctgggtc gtcaccacga tgaagccggg cgaccgcccg ctctacaacc tcgccgccgc
     2281 ggtgctcgaa gcgatcggcc tcgacccgac cgccgccgac ttcaccgcgc gcacagacgg
     2341 gctcgtcgaa gaggtcggcg ccgagggcgt ccgcgccttg accgcgcggc tcgcgcccgt
     2401 gctcggccac cgcgacgcga acctcctgct gctcgtcgat cagttcgagg aaatcttccg
     2461 cttcggcgcg cgcgcgcgcg acgacgacga cgacgcgggc gagcaggacg cggcggcggt
     2521 gaggcagcgc gaccgggccg ccgacttcgt ctcgctcatg ctcgcgctct cggagcagag
     2581 ggacgtgccg gtctacgtcg taatgacgat gcgctcggac ttcctcggcg actgcgacgc
     2641 cttccgcggc ctgcccgagg cgatgaacca cagccagtat ctcgtcccgc gcctctcgac
     2701 gcagcagcgc gaggaggcca tcagcggccc catccgcctc tccggcgccg cgatcacgcc
     2761 gcgcctcttg gacctcgtcc gcaacgacgt gggcgacaag ctcgaccagc tccccgtcat
     2821 gcagcacgcc atgatgcgga cgtgggagaa ctggcgcgtc ggcgacggcg gccccgtgga
     2881 cgtgcccgac tacctcgccg cggggacgat ccggaacgcg ctctcggacg aggccgaaga
     2941 cgtgctgcgc gcgatggacg aggagggcgg cggccagcgc aagctgacga tgctcgtctt
     3001 tcaggcgctc accaacaccg acgccgagaa ccgccgcatc cgccgcccga agcggctgcg
     3061 cgaactcgaa gccatcacgg gggccggcgg cgagcagatc atgaagatcg tctctctctt
     3121 cgagggcggc gggcgctcgt tcctcaccgt cgcgcgcgac cacgcccgcg gcaaccacct
     3181 catcgacatc tcgcacgaga gcctcatccg ccagtggaag acgctgcgcg agtgggtgga
     3241 caaggaggcc gacggg
//
LOCUS       DBNGOH010000857         5778 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1231006, whole genome shotgun sequence.
ACCESSION   DBNGOH010000857 DBNGOH010000000
VERSION     DBNGOH010000857.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5778)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5778)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5778
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1231006"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..943)
                     /locus_tag="AC159P_16570"
     CDS             complement(<1..943)
                     /locus_tag="AC159P_16570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3697109.1"
                     /translation="MAESIPIAPTTLKIEAPWRERLFPNNEWVLIVVLVVECAIFSVT
                     GNNFLSSSNAFEITRLSVEIGLLALALTPIIITGGIDLSVGSMMGLAAVVLGALWRDA
                     GLPLPVAASIALLVGVGGGGLNALMITRLKFPPLIVTLGTFSLFRGVAEGLTGGVENY
                     SGFPQSFLFLGQGYVGGVIPTQLFILIAAIAGYWWWLQRTAAGRSLYAIGFSAEGARY
                     AGLPVGRRLGAVYVLSGLASSLAAIIYVAHLGQAKSDAGTGYELMAITAVVLGGASIF
                     GGRGTVLGTVLGLFAIVILQNGLRLSAQPAELAGILTG"
     gene            complement(951..1913)
                     /locus_tag="AC159P_16575"
     CDS             complement(951..1913)
                     /locus_tag="AC159P_16575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014784296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3697110.1"
                     /translation="MNLSRYKRELSAALAFVALLVLVAVREPTFFSGANLRDLALNNA
                     PVLLIAVGMTLVILVGEIDISVGSQFAVCSVAAGLLAKAGVPVLLLLPCVVVIGAMMG
                     ALNGVLVGHLRLPSIIVTLAMLVAWRDALRWTTEGAWVQDLPPDFQWFGLGQGAGQLL
                     IVAVALAVLVAFSWISRNLGAGRAVYAVGSDAEAARLAGIEPPRVVFGVFLLMGALVG
                     LAALLNAVRFSAVPSNAGLGLELKAVAAVVVGGTAITGGRGTLVGTLIGVALLGTIGT
                     ALTFLGINPFWERAIQGGIILAALVADVALARLERHGAKLSPAR"
     gene            complement(1931..3415)
                     /gene="glgA"
                     /locus_tag="AC159P_16580"
     CDS             complement(1931..3415)
                     /gene="glgA"
                     /locus_tag="AC159P_16580"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012121282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - alpha-1,4-glucan
                     glucosyltransferase (UDP-glucose donor) activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HIR3697111.1"
                     /translation="MEPLKIALIASECVPFAKTGGLADVAGALPAALAALGHEVIVVV
                     PKYQKVDAGKHGLRRHLHELGVWMGDALEWCAVDVADSAAGVPVYFIENDRYFQRAGL
                     YDDPASHREYADNPRRFGFFTRAALQLCRDTGFAPDIVHVHDWPTALAPAYLKTWHWN
                     DPVLGPAASVLTIHNIAYQGVYGAESYGYLGLQARNFTPDKFEDHGRINFLKGGIHFA
                     DMVNTVSPTYARETRTPAFAYGMAPQLNDKGANYVGILNGVDYADWNPAVDALIPAQY
                     TPEDLSGKAACKAELQKTFLLEADPDIPVVGVVSRLEKEQKGLDLLATAVESIARNMR
                     VQFAILGSGDKGLEGVYRNLPARYPGRVGSYIGFDNRLAHLIEAGSDFFVMPSRYEPC
                     GLNQMYSLKYGTLPIVRATGGLNDTVEQYDERTGAGTGFKFDEPSARAIYYTVGWAVS
                     TYYDRRPHIRQMIRNAMAQDFGWEASARRYERLYERAVANKKAL"
     gene            complement(3429..4970)
                     /locus_tag="AC159P_16585"
     CDS             complement(3429..4970)
                     /locus_tag="AC159P_16585"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HIR3697112.1"
                     /translation="MTESVLLRAADITKSYAGVQALKSGSIELRAGEVHALIGENGAG
                     KSTLIKIMTGAVAPDSGEIVLNGEPVVNNSPRLAKSLGIIAIYQQPALFPELTVAENI
                     AIGLEQSGMLGRVDWKERRRRAAELLARVGARIDPDTAAGDLTMPQQQLVEIARALGA
                     EAKVLILDEPTASLSEEDTQNLFRVIRELRAAGVGMIYISHRLEELPVIADRVTVLRD
                     GRTIDTRPMSEVNRQQLIQLMVGRELSAVFPKREVELGDVVLELRRVGCSESGVNTVS
                     LELRAGESLGLAGLVGAGRTELARTVFGLTPADEGEISLRGQPVKLDSPAAAIKAGIA
                     YVPEDRRRHGVILDLPISNNITLAALDQLTRGGALDFRKEREVAADYTRRLGVKTPAI
                     YVPVATLSGGNQQKVALSRWLMTKPSVLILDEPTQGIDVGAKSEIHSLMTDLAAEGVA
                     ILMISSELPEILGMSDRIAVMHGGTIFGVLDRAEATQNKILALALGHELTDTGARAAE
                     TPQTV"
     gene            complement(4963..5676)
                     /locus_tag="AC159P_16590"
     CDS             complement(4963..5676)
                     /locus_tag="AC159P_16590"
                     /inference="COORDINATES: protein motif:HMM:NF014628.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutC/YkgG family protein"
                     /protein_id="HIR3697113.1"
                     /translation="MAERTHNDDARREMMAAIRRHLAASAPFDTVREEHHAGHGGAVG
                     KGRVIEALREPEASPVERFGRALEAVAGRCVVARDVAAAAEAVSEIFAAKKARRVAVS
                     DSELARRVLALADADVELVETAAAADLFHCDVGVTGAQWAVAETGTLVLEAESERHRL
                     ASLVPPVHVAVVGAGRVRQTLAEVLASLGERGRDRLSRTVTFVTGPSRTSDIELTLAI
                     GVHGPGELYVVIIEGEGHD"
BASE COUNT          922 a         2114 c         1853 g          889 t
ORIGIN      
        1 cgcccgtcag gatgcccgcc agctccgccg gctgcgcgct caaccgcagc ccgttctgca
       61 aaatcacgat ggcgaacagc ccgagcacag tgcccagcac tgtcccgcgc ccgccgaaga
      121 tcgacgcccc gccgagcacg acggcggtga tcgccatcaa ctcgtagccg gtgcccgcgt
      181 ccgacttcgc ctgccccaaa tgcgcgacgt agatgatcgc cgcgaggctc gacgccagcc
      241 ccgacagcac gtagaccgcg ccgaggcgcc gccccacggg cagccccgcg tagcgcgcgc
      301 cctccgccga aaagccgatg gcgtacaggc tccgcccggc cgccgtgcgt tgcagccacc
      361 accagtaacc ggcgatggcc gcgatcagga tgaacaactg cgtcgggatg acgccgccga
      421 cgtacccttg ccccagaaac aggaacgact gcgggaaccc cgaatagttt tcgacgccgc
      481 ccgtcagccc ctcggcgacg ccgcggaaca aagagaacgt ccccagcgtc acgatcagcg
      541 gcgggaactt cagccgcgta atcatcaacg cgttcagccc gccgcccccg acgcccacga
      601 gcagagcgat cgacgcggcg accggcaacg gcagccccgc gtcgcgccac aacgccccga
      661 gcacgaccgc cgccagaccc atcatcgagc cgaccgacaa gtcgatcccg cccgtgatga
      721 tgatcggcgt cagcgccagc gccagcagcc cgatctcgac gctgagccgc gtgatctcga
      781 acgcgttcga cgagcttaaa aagttgttcc ccgtcacgct gaagatcgcg cactcgacca
      841 cgagcacgac gatcagcacc cactcgttat tcgggaacag gcgctcgcgc cacggcgcct
      901 cgatcttcaa agtcgtcggt gcgattggga ttgattccgc catcgtcagt tcatcttgcc
      961 ggactgagtt tcgccccgtg gcgttcgagc cgcgccagcg ccacgtccgc gaccagcgcc
     1021 gccagtatga tgccgccctg aatcgcacgt tcccagaacg ggttgatgcc taaaaaagtc
     1081 agcgccgtcc cgatggttcc caggagcgcc accccgatca gcgtcccgac gagcgtcccg
     1141 cgcccgcccg tgatggccgt cccgccgacg accaccgccg ccaccgcctt caactccaaa
     1201 cccagccccg cgttgctcgg cacggcgctg aaccgcaccg cgttcagcag cgccgccagc
     1261 cccaccagcg cccccatcaa caagaacacg ccgaacacca cgcgcggcgg ctctatcccg
     1321 gcgagccgcg ccgcctccgc gtccgacccg accgcgtaga ccgcgcgccc cgcgcccaga
     1381 tttctcgaaa tccaactgaa cgccaccagc accgccagcg cgaccgccac gatgagcagt
     1441 tgccccgcgc cctgccccag cccgaaccac tggaagtccg gcggcaagtc ctgcacccac
     1501 gcgccctcgg tcgtccaacg cagcgcgtcg cgccaagcca ccagcatcgc cagcgtcacg
     1561 atgatcgaag gcagccgcag gtgccccacc agcaccccgt tgagcgcgcc catcatcgcg
     1621 ccgatgacga cgacgcacgg cagcagcaac agcaccggca cgcccgcctt cgccagcagc
     1681 cccgccgcca cgctgcacac cgcgaactgc gaccccacag agatgtcgat ctccccgacc
     1741 agaatcacca gcgtcatccc gaccgcgatc aacaacaccg gcgcgttgtt cagcgccaga
     1801 tcgcgcaggt tcgccccgct gaagaacgtc ggctcgcgca ccgccaccag caccagcagc
     1861 gccacgaacg ccagcgccgc cgacaactca cgcttgtaac gactgagatt cacttctttc
     1921 gtgcctcgtc ctacagggcc ttcttgttgg cgacggcgcg ctcgtagagt cgctcgtacc
     1981 ggcgcgcgct cgcctcccag ccgaagtcct gcgccatcgc gttccggatc atctgccgga
     2041 tgtgtgggcg gcggtcgtag taggtgctga cggcccagcc gacggtgtag tagatggcgc
     2101 gggcgctcgg ctcgtcgaac ttgaagccgg tgcccgcgcc ggtgcgctcg tcgtactgct
     2161 cgaccgtgtc gttgaggccg cccgtggcgc ggacgatggg cagcgtgccg tacttgagcg
     2221 agtacatctg gttcagcccg cacggctcgt agcgcgaggg catgacgaag aagtcgctcc
     2281 ccgcttcgat caggtgcgcc agcctgttgt cgaagccgat gtagctgccg acgcgccccg
     2341 gatagcgcgc gggcaagttg cggtagacgc cttccagccc cttgtcgccg ctcccgagaa
     2401 tggcgaactg cacgcgcatg ttgcgggcga tgctctcgac ggctgtggcc aggaggtcga
     2461 gacctttctg ctctttctcc agacggctga cgacgccgac gacggggatg tccgggtcag
     2521 cttcgagcag gaaggtcttc tgtagctcgg ccttgcaggc ggccttgccc gacaaatctt
     2581 cgggcgtgta ttgcgcgggg atgagcgcat cgaccgcggg gttccagtcc gcgtagtcca
     2641 cgccgttgag gatgccgaca tagttcgcgc ccttgtcgtt gagctgcggc gccatgccgt
     2701 aggcgaaggc gggcgtgcgc gtctcgcgcg cgtaggtcgg gctgacggtg ttgaccatgt
     2761 ccgcgaagtg gatgccgccc ttgaggaagt tgatgcgccc gtgatcctcg aacttgtcgg
     2821 gcgtgaagtt gcgcgcttgc agaccgaggt agccgtagct ctccgcgccg tagacgccct
     2881 gataggcgat gttgtgaatc gtgagcacgc tcgccgccgg gccgagcacg gggtcgttcc
     2941 agtgccacgt tttcagatag gccggggcga gcgcggtcgg ccagtcgtgg acgtggacga
     3001 tgtcgggcgc gaagcctgtg tcgcggcaga gctggagcgc ggcccgcgtg aagaagccga
     3061 agcgccgcgg gttgtccgcg tattcgcggt gcgaggcggg gtcgtcgtac agccccgcgc
     3121 gctgaaagta gcggtcgttc tcgatgaagt agaccggcac gcccgcggcg ctgtccgcga
     3181 catcgacggc gcaccattcg agcgcgtcgc ccatccacac gcccagctcg tgcagatgac
     3241 gacgaaggcc gtgcttcccg gcatcgacct tctgatactt cgggacgacg acgatcacct
     3301 cgtggccgag cgccgcgagc gcggcgggca gcgcgcccgc cacgtccgcc aggccccccg
     3361 tcttggcgaa cggcacgcat tcggaagcga tcaacgcgat cttcagtggc tccatgctcc
     3421 ccttagactc agacggtctg cggcgtctct gcggcgcgcg cgcccgtgtc ggtcaattcg
     3481 tggccgaggg cgagcgcgag gattttgttc tgcgtcgcct ccgcgcggtc gagcacgccg
     3541 aagatggtgc cgccgtgcat gacggcgatg cggtcgctca tgccgaggat ttccggcagc
     3601 tccgaagaga tcatcaggat ggccacgcct tcggcggcga ggtcggtcat cagcgagtga
     3661 atctcggact tcgcgcccac gtcgatgcct tgcgtcggct cgtcgaggat gagcaccgac
     3721 ggcttcgtca tcagccagcg cgagagcgcg accttctgct ggttgccgcc cgagagcgtg
     3781 gcgaccggca cgtatatcgc gggcgtcttc acgccgagcc gccgcgtgta gtcggcggcc
     3841 acctcgcgct ctttgcggaa gtcgagcgcg ccgccgcgcg tgagctggtc gagcgccgcg
     3901 agcgtgatgt tgttgctgat cggcaagtcg agaatgacgc cgtggcggcg gcggtcttcg
     3961 ggcacgtagg cgatgcccgc cttgatggcc gcggcggggc tgtcgagttt gacgggttga
     4021 ccgcgcaaac tgatctcgcc ctcgtcggcg ggcgtgaggc cgaagacggt gcgcgccagt
     4081 tccgtgcgcc ccgcgccgac gaggcccgcg aggccgaggc tttcgcccgc gcgaagctca
     4141 aggctcacag tgttgacgcc gctctcggag cagcccacgc ggcgcagctc caacaccacg
     4201 tcgcccagct cgacttccct cttcgggaag accgccgaca gctcgcgccc gaccatcagt
     4261 tggatgagct gctggcggtt cacctccgac atcgggcgcg tgtcgatcgt ccgcccgtcg
     4321 cgcaacaccg tcacgcggtc ggcgatgacg ggcagctctt ccagacggtg cgagatgtag
     4381 atcatcccga cgcccgccgc gcgcagctcg cggatgacgc ggaagaggtt ctgcgtgtcc
     4441 tcttccgaaa gggacgcggt cggctcgtcg aggatcagga ctttggcctc ggcgccgagc
     4501 gcgcgcgcga tctcgacgag ctgctgctgc ggcatcgtga ggtcgcccgc cgccgtgtcc
     4561 gggtcgatcc gcgcgccgac gcgtgccagc agctccgcgg cccgccgccg ccgctccttc
     4621 caatcgacgc gcccgagcat ccccgactgt tcgaggccga tggcgatgtt ctcggcgacg
     4681 gtgagctcgg ggaagagcgc gggctgttga taaatggcga tgatgccgag cgacttggcg
     4741 aggcgcggcg agttgttcac gaccggctcg ccgttgagga cgatctcgcc ggagtcgggc
     4801 gcgaccgcgc cggtcatgat tttgatgagc gtacttttac ccgcgccgtt ctcgccgatg
     4861 agcgcgtgca cctctcccgc gcgcagctcg atggagccgc tcttcaaagc ctgcacgcct
     4921 gcgtacgact tggtgatgtc ggcggcgcgg agtagcacgc tctcagtcat gtccctcgcc
     4981 ctcgatgatg acgacgtaca gctcgcccgg cccgtgcacc ccgatggcca gcgtcagctc
     5041 gatgtcggaa gtgcgcgacg gccccgtgac gaaagtcacc gtccggctca gccggtcgcg
     5101 cccccgctcg ccgagcgatg cgagcacctc ggccagcgtc tgccgcacgc gccccgcgcc
     5161 gacgacggcg acgtggacgg gcggcaccag cgaggcgagg cggtgccgct cgctctcggc
     5221 ttcgagcacg agcgtgccgg tctcggccac ggcccactgc gcgcccgtca cgcccacgtc
     5281 gcagtggaag aggtctgccg ccgccgccgt ctcgaccagc tccacgtcgg cgtccgcaag
     5341 cgcgagcact cttcgcgcca gctccgaatc cgagaccgcg acgcgacgcg ccttcttcgc
     5401 cgcgaagatt tcactcacgg cctccgccgc cgcggccacg tcgcgcgcca ccacgcagcg
     5461 ccccgccacc gcttcgagcg cccgcccgaa acgctcgacc ggcgacgcct ccggctcgcg
     5521 caaggcttcg atgacgcgcc ccttgcccac ggcgccgccg tgccccgcgt ggtgctcttc
     5581 gcgcacggtg tcgaacggcg cgctcgccgc caggtgcctg cgaatcgccg ccatcatctc
     5641 gcgccgcgcg tcgtcgttgt gtgtgcgttc ggccaagttc ttttccggtg ccgcccgttg
     5701 acgttggaac ataaagcaag cgcgcgtcat tccgccgcgt agcggcgatt gaatgtagcc
     5761 cggcgtttca acgccggg
//
LOCUS       DBNGOH010000858         3592 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4161507, whole genome shotgun sequence.
ACCESSION   DBNGOH010000858 DBNGOH010000000
VERSION     DBNGOH010000858.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3592)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3592)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3592
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4161507"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..538)
                     /locus_tag="AC159P_16595"
     CDS             complement(<1..538)
                     /locus_tag="AC159P_16595"
                     /inference="COORDINATES: protein motif:HMM:NF012698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HIR3697114.1"
                     /translation="MSGQEKHFYQFGPFRLDPVKRRLLRDGGPVPLTPKAFDTLLALV
                     EQSGRTVEKDELMRRVWPGAVVEENNLNQNITALRKTLGDSRQESQYIATVPGLGYRF
                     VADVREVEAEGADVLGGGERTRGASGAEGEDSVAGSAEASGGAAPPAQAPLALAAATE
                     AAPPEPVRPRQPPAGKSAL"
     gene            979..2142
                     /locus_tag="AC159P_16600"
     CDS             979..2142
                     /locus_tag="AC159P_16600"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0140905 - haloperoxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vanadium-dependent haloperoxidase"
                     /protein_id="HIR3697115.1"
                     /translation="METVSTPGQHPPTIMPVRSYAMMHAAMFDAVNSIDGTYTPYLTD
                     VPGSPNASLEAAAAQAAHDVLAALYPTRREVFDAELAASLEGIEEYRAQQGIRVGQIV
                     AERLLATRADDGWNVTPPSYTLPPTPGNWQPTPPGNAAATFTHYGSVLPFAITSSTQF
                     APNPPPALTSAEYAAALNEVKEIGAVNSATRTADQTLVARLWAGVGTPTNFLFVWNNV
                     ARTVALDRGITTVEMARLFALTNISLHDALQTSFASKFQHGMWRPVTAIRRADEDDNP
                     DTTPDAAWSSLIVAPPYPTYAGNMATIGTSQSTTLAQFFGRDDIRFQHTWQGAGGATR
                     SYAGFGAMADEQARARVYGGIHFTFDNVAGQSIGQNVANYVFRNLMRPRQCVR"
     gene            2675..>3592
                     /locus_tag="AC159P_16605"
     CDS             2675..>3592
                     /locus_tag="AC159P_16605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697116.1"
                     /translation="MKRGSPQGLAHRIRATVAAFALLGLVCVLAHELSPEPVGATSPA
                     SPDSISNNRPAPNPGGAAATFSTQGAVDLTGEYFQAQGTNGRSCATCHIPEEAWGINP
                     GTLQRLFNETDGTHPVFNELDANNPDNIDQNSTVEELLDGYSMLLTRGVFRRGGAPRP
                     NSEWELIAVDDPHGFANTGRLVHWRRVMPTINFPVGSATINWDGGNSIGTNVHAGLLN
                     QAGRNISGAQAGPTPAPTPVVEDIVAFEESLFTAQLIVPGIGRLDSDGARGGPEALST
                     MPKVAGRFDLFDAWAGHENPRRAQIARGQA"
BASE COUNT          643 a         1255 c         1133 g          561 t
ORIGIN      
        1 ggagcgccga cttaccggca ggcggctgcc gggggcgaac cggctcgggc ggcgccgctt
       61 ccgtcgccgc cgcgagcgcg agcggcgcct gcgctggcgg cgccgcgccg cccgatgcct
      121 ccgccgaccc ggctacagag tcttctccct ccgcgccgct cgcgccccgc gttcgctccc
      181 cgccgccgag aacgtcggcg ccctcggcct ccacctccct cacgtccgcg acgaagcggt
      241 agccgagtcc ggggacggtg gcgatgtact ggctctcttg acggctgtct ccgagcgtct
      301 tcctcaaggc ggtgatgttc tggttgaggt tgttctcctc cacgaccgcg ccgggccaga
      361 ccctccgcat caactcgtcc ttctcgaccg tcctgccgct ctgctcgaca agcgcgagca
      421 gtgtgtcgaa cgccttcggg gtgagcggca ccggcccgcc gtcgcgcagc aggcggcgtt
      481 tgacggggtc gagacggaac ggcccgaatt gatagaagtg cttttcctgc ccagacatag
      541 ccgctttcag aaactctttc agaaaaaatc agcgggttcc taagtacatc cgcaccgctc
      601 ccgaggtagt ttccgccgca cgatgaggcc gcggcgttcg cggcgggttg ggagatgccg
      661 ccggaattct ctgcgccggc agtaatacgg cggagagttc agcaacttca tcttgcgaca
      721 ggcgctcatt gtagcgccgc caaattgacc ggagcaatcc ggtgagactg agggcggaag
      781 cggtagaaag caacgcccgt tgaaacaacc tgacagcagg aggagaaaaa gaggatgact
      841 tacaaacgga ttgtcgaatg tcttctgacg gtggcggttc tactttgcgc ccagatgccg
      901 gccctggcaa atgacagtgg cgcggcaaag ctgcttgtgc cgcaggaaga tgtgattttg
      961 cagtggaacc gtgtgttgat ggaaaccgtc agcacccccg gacagcaccc gccgaccatc
     1021 atgcccgtgc gcagctacgc gatgatgcac gcggcgatgt tcgacgccgt caattccatc
     1081 gacgggactt acacgcccta tctgacggac gtgccggggt cgcccaacgc ctcgctcgaa
     1141 gccgccgccg cgcaagccgc gcacgacgtt ttggcggcct tgtacccgac ccggcgggag
     1201 gttttcgatg cggagttggc cgcttcgctc gaagggatcg aggaataccg ggcgcagcag
     1261 ggcatcaggg tcgggcagat tgtcgccgag cgcctgttgg caactcgcgc ggacgacggc
     1321 tggaacgtga cgccgccgtc atacacgctg ccgccgacgc cgggcaactg gcaaccgacc
     1381 ccgccgggca acgccgccgc gaccttcacg cattacgggt cggtcttgcc gttcgccatc
     1441 acttcgagca cgcagttcgc ccccaacccg ccgcccgcgt tgacgagcgc ggagtacgcc
     1501 gccgccctta acgaggtcaa ggaaatcggc gcggtgaaca gcgcgacgcg caccgccgac
     1561 cagactttag tggcgcggct ctgggcgggc gtcggcacgc cgaccaactt ccttttcgtc
     1621 tggaacaacg tcgcccgcac ggtcgccctc gaccgcggca tcacgaccgt cgaaatggcg
     1681 cgcctgttcg ccctgacgaa catctcgctt catgacgcgc tgcaaacctc gttcgccagc
     1741 aaattccagc acggcatgtg gcgccccgtc acggcgattc gccgcgccga cgaggacgac
     1801 aacccggaca cgacgccgga cgccgcgtgg tcgtctctga tcgtcgcccc gccctatccg
     1861 acctacgccg gaaacatggc gaccatcggc acttcgcaat cgaccacgct cgcgcagttt
     1921 ttcgggcgcg acgacatccg cttccaacac acctggcaag gcgcgggcgg cgccacgcgc
     1981 tcgtacgcgg ggttcggggc gatggccgac gagcaggcgc gggcgcgcgt ttacggcggc
     2041 atccatttca cgttcgacaa cgtggccggg cagtccatcg gccagaatgt cgccaactac
     2101 gtcttccgga atctcatgcg accgcgccaa tgcgtccgct aggggttttg tcggtcgcac
     2161 atcgtagtga gcggctaagc tcacaacgat gtgcgaccgt tgcgtggaga acttcgctct
     2221 cctcgccacc tcgacacggt aaaggagaaa ggtcatgcac gacgtgaagt taaaacgcct
     2281 catccggcgt gtccatctca ctactcgctt ccgatgcgcg aggcggacga gctgacatcg
     2341 gcgggccgcg tccgtgttga aaaccgcggc gcggttcgcc gcgagaaaaa atcatgtcgc
     2401 aaaaaatcca tgccgccgtg atcccctgcg caccccgttt tcgcgttagc aagtgcgggc
     2461 ggcagcgggc cgtgggccgg gtcgcccgtc cgacacgccg gacacgaaga gagtccggca
     2521 cgaaccgccc caagacctcg ctcatccgca ggctcagtgc ccccgctgcc cccgcgccgc
     2581 agaccgttga gaacgccccg aatccggcag gccaacttca ctttcggtct gtcggagcca
     2641 ttccgatcag tgatgagatg aggaggacat tacgatgaag agaggtagcc cgcagggtct
     2701 cgctcaccgg atacgggcga cggtcgctgc gttcgcattg ctcggtctgg tgtgcgtgct
     2761 cgcacacgaa ctgagtcccg agccggtcgg cgcgaccagc ccggcgtcac cggacagcat
     2821 ctccaacaac cgaccggcgc cgaaccccgg cggcgccgcc gcgaccttca gcacgcaggg
     2881 cgccgtcgat ctcaccggcg aatatttcca ggcgcagggc acgaacgggc ggagctgcgc
     2941 cacctgccac atcccggagg aggcgtgggg catcaacccc ggcacgctcc aacgcctgtt
     3001 caacgagacg gacggcacgc acccggtctt caacgaactc gacgccaaca acccggacaa
     3061 catcgatcag aactccaccg tggaggaact cctcgacggc tacagcatgc tcctcacacg
     3121 cggcgtcttc cgccgtggcg gcgcgccgcg gcccaactcc gagtgggagc tgatcgccgt
     3181 ggacgacccg cacggcttcg ccaacacggg ccggctcgtc cactggcggc gcgtcatgcc
     3241 gacgatcaac ttccccgtcg gcagcgcgac catcaactgg gacggcggca acagcatcgg
     3301 caccaacgtg cacgcagggc ttttgaacca ggcggggcgt aacatctcgg gcgcacaggc
     3361 ggggccgacc ccggcaccca ctcccgtcgt cgaagacatc gtcgccttcg aggaatcgct
     3421 cttcaccgcg cagctcatcg tgccgggcat cgggcggctc gactcggacg gcgcgcgcgg
     3481 cggccccgag gcgttatcga ccatgccgaa ggtcgccggg aggttcgacc tcttcgacgc
     3541 gtgggccggc cacgagaacc cgcggcgcgc gcagatcgcg cgcgggcagg cg
//
LOCUS       DBNGOH010000859         2638 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1231261, whole genome shotgun sequence.
ACCESSION   DBNGOH010000859 DBNGOH010000000
VERSION     DBNGOH010000859.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2638)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2638)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2638
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1231261"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            315..974
                     /locus_tag="AC159P_16610"
     CDS             315..974
                     /locus_tag="AC159P_16610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697117.1"
                     /translation="MNRTFRLVVLSVVTGVVASALTHTAWGRQTGRRGAGNSAEVERL
                     ERERRDREMREADLRERQLLLRNMKPDARPAERPTPRLATAQIREDFVRLQVVNNDLA
                     KAGSRADALDLKFVSKSAAEIRRLAARLRDNLALPGAGDAPEHPVVKAAPPAQLGPAL
                     SALDGLILNFADGLASKGVYLVDAEASAKVRRELEAIIELSGWVKKASERLEKSGRRP
                     R"
     gene            complement(1217..2560)
                     /locus_tag="AC159P_16615"
     CDS             complement(1217..2560)
                     /locus_tag="AC159P_16615"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0140905 - haloperoxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vanadium-dependent haloperoxidase"
                     /protein_id="HIR3697118.1"
                     /translation="MKVKTRSTGLVIAIGLGVALLACAASVPVGGVRAVAGRSPVAAQ
                     PAALGDAVMEWNQQAATRTLTKPAPALAPVEQTRVMAIAQVAVHDAVNGITGEYATYL
                     SPGPAPASASPEAAAIAAAHHALKNLPVNQSPSLDDAFAASLAARGLSEFDPGVEYGR
                     SAASAILAARAGDGSAQAQFPYSAPGAGSPGVWVPISSAPGAQALLPGWGSVTPFVLR
                     SGSQFRPEAPPALDSEQYAKDYNEIKTIGAAGSPVRTALQTDIATFWLASPTAIWNPV
                     LRQAVAAQNLDLSAEARAVALFYLAASDAGVACWDAKYEHNFWRPQLAIRGGDTDDND
                     LTTGDGTWTPLHGTPRHPEYPSGHTTNSSAMATILGLLFGEGTGAPIVVTLGSITRQW
                     DTFDEAAAEVIDARVYSGIHFRTADEVGARLGRQVAQFTWTHALRPTPRGRSERP"
BASE COUNT          422 a          889 c          905 g          422 t
ORIGIN      
        1 cccggcgttc tgctcggcga aagcgaaaac ctgaagcagg ttcgttatct caagataggt
       61 tccgtcgagg atttatattc cgaagagacg ctccggattt tgcacgaagc aatcatcctt
      121 gatgaaatga tggctaagag gcactaataa aaataggcga ttatcccctt gacaaagtag
      181 ggcccctata ctttgtcaaa gggataatcg ccaaactctt gtcaccaatc gccgcttcac
      241 agagagttgc ttgcggaacc ttccagaacc cgcggttagc gttcatactc cgcgacggag
      301 ggaggaggta agagatgaac cgcacgttca gactcgtggt gttgtccgtt gtgaccgggg
      361 tcgtcgcctc ggccctcacc cacaccgcct ggggccgtca gacgggcagg cgcggcgccg
      421 gcaactcggc agaggtcgag cgccttgagc gcgagcgccg cgacagggag atgcgcgagg
      481 ccgacctgcg cgagcgccag ctcctgctcc ggaacatgaa acccgacgcg cggcccgcgg
      541 agcggccaac cccgcggctc gccacggcgc agatcaggga ggacttcgtc cgcctacagg
      601 tcgtcaacaa cgacctggcc aaagccggct cccgtgcgga cgcgctcgac ctcaagttcg
      661 tgtccaagtc agccgccgag atcaggagac tggcggcgcg gctcagggac aacctcgcgc
      721 tgccgggggc gggggacgct cccgagcatc ccgtggtgaa ggccgcgccg cccgcgcaac
      781 tgggcccggc gctctcggcg ctcgacgggc tgatcctcaa cttcgccgac ggcctggctt
      841 cgaaaggcgt ctacctagtc gatgccgaag cctcggccaa ggtgcggcgc gaactcgaag
      901 cgatcatcga gctgagcggg tgggtgaaga aagccagcga gagattggag aagtcgggcc
      961 gccgcccccg ctgaggccca cgcccggcac cacccgcggc gcacgcaccc acgccagggc
     1021 gcgcggtgtc cctgctcttt gcaccagaac ccttgtggat aattggagcg gtgggaatgc
     1081 gaaagcgctg cctctggggc agcgctttcg catatgcgta ttcaacaccc agtcgaacgg
     1141 gacggtttcg tgcctccgtc ccgtcagctt taccacgctc gtcttgacag tcaacgtgcc
     1201 cccagccgtg gcaatcctag gggcgttcag accttcccct cggcgtgggc cgcagcgcgt
     1261 gcgtccaggt gaactgtgcg acctgccgcc cgagcctcgc gcccacctcg tccgccgtgc
     1321 ggaagtgaat ccccgagtag acccgcgcgt cgatcacctc cgcggcggct tcatcgaatg
     1381 tgtcccactg ccgcgtgatg ctccccagcg tgacgacgat gggcgcgccc gtcccctcgc
     1441 cgaacagcag cccgagaatg gtggccatcg cgctgctgtt cgtcgtgtgc cccgacgggt
     1501 actccgggtg ccggggcgtc ccgtggagcg gcgtccatgt cccgtcgccc gtcgtcaggt
     1561 cgttgtcgtc cgtgtcgccg cctcggatgg cgagctgcgg acgccagaag ttgtgctcgt
     1621 acttcgcgtc ccagcaggcg acaccggcgt cggacgccgc caggtagaag agcgcgacgg
     1681 cgcgcgcctc tgccgagagg tcgaggttct gcgccgcgac ggcctgccgc agcaccgggt
     1741 tccagatggc ggtcggggag gccagccaga acgtcgcgat gtccgtctgg agggccgtcc
     1801 gcacggggct gcccgccgcg ccgatggttt tgatctcgtt atagtccttc gcgtactgct
     1861 cgctgtcgag cgccggcggg gcctcggggc ggaactgcga gccgctgcgc aacacgaacg
     1921 gagtgacgct gccccacccg ggcagaagcg cctgcgcgcc gggcgcgctg gagatcggca
     1981 cccagacgcc gggcgatccc gcccccgggg cagagtaagg gaactgggcc tgggccgacc
     2041 cgtcgccggc gcgcgccgcc aagatggcgg acgccgccga gcgcccgtac tcgacgccgg
     2101 ggtcgaattc cgacagcccg cgcgcggcga gcgacgccgc gaaggcgtcg tcgagcgacg
     2161 gggactggtt gaccggcagg ttcttcagcg cgtggtgcgc ggcggcgatg gccgccgcct
     2221 cgggcgaggc gctggcgggc gcggggccgg gcgagaggta ggttgcgtac tcgcccgtga
     2281 tgccgttgac ggcgtcgtgg acggcgacct gtgcgatggc catgacgcgc gtctgctcga
     2341 ccggcgcgag cgccggcgcg ggcttcgtca gggtgcgagt ggcggcctgc tggttccact
     2401 ccatgacggc gtcgcccagg gccgcgggct gcgcggcgac cggactcctt ccggccaccg
     2461 cgcgcacgcc gccgaccggc acggacgcgg cgcacgccag gagggcaacg ccgaggccga
     2521 ttgctatcac gagtcctgtg ctccgtgttt tgactttcat gttcgatctc ctttcacttc
     2581 gttggcatag tcgatttcgc gtacgtcgct cgcggatgca agaggcggat acctttcg
//
LOCUS       DBNGOH010000860         2046 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_5079667, whole genome shotgun sequence.
ACCESSION   DBNGOH010000860 DBNGOH010000000
VERSION     DBNGOH010000860.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2046)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2046)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2046
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_5079667"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..948
                     /locus_tag="AC159P_16620"
     CDS             <1..948
                     /locus_tag="AC159P_16620"
                     /inference="COORDINATES: protein motif:HMM:NF012428.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HIR3697119.1"
                     /translation="YSDVRAEAAEKLAGCAPAEMTKAFFCNSGTEANENAMRMARMKT
                     GRESVITFTGGFHGRTADAISATFLGKYREIGRPNVPGHLCAEFGSIESVAALADETV
                     AAVMLEPIQSMAGVAVAAPEFYVALRRLCEERGMLLIYDEVQTGVGRTGEWFFAGSEA
                     SGAAVPDIITLAKALGSGVPVGACLTTEAVAAAIKENDLGTTFGGGMLAMAAVNATLG
                     AIEEDGMLENARAVEAFLRESLAGVERVAALRGRGLLLGVEFMEPIASDVQRALLKRR
                     IITGTSSDPRVLRLLPPLCVSVEEARLFVEALGEVLAEG"
     gene            945..1943
                     /locus_tag="AC159P_16625"
     CDS             945..1943
                     /locus_tag="AC159P_16625"
                     /EC_number="2.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019463760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_function: GO:0043857 - N-acetylornithine
                     carbamoyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylornithine carbamoyltransferase"
                     /protein_id="HIR3697120.1"
                     /translation="MTRHFLTTADYTREELEGLLAAALRFKRGEDRSRPLAGRSVALV
                     FFNPSLRTRASMQVGVYELGGNAVILEPGGTSWTLEHRDGIVMDGDKTEHVAEFVRVL
                     GRYASLMGVRTFAQLKDWEEERLDPILNAFAKYSEVPVVNLESAMHHPCQAMADMLTV
                     REKLGEGPKRVVLTWAWHPKPLPMAVPNSFALAAAQMGHELTIAHPPGYELDEELIQD
                     INLRAFDAGAKVTVTNDIEEAFDGAEAVYAKSWGGRMFYGSAAEDIEERAQYRDRWIV
                     DAAKMRRTAGGIFMHCLPVRRNCIVTDEVIDSSASVVIDEAENRLHVQKAIITKLL"
BASE COUNT          346 a          600 c          770 g          330 t
ORIGIN      
        1 tactcggacg tgcgcgcgga ggcggccgag aagttggccg ggtgcgcgcc cgcagagatg
       61 acgaaagcct tcttctgcaa ctcgggcacg gaggcgaacg agaacgcgat gcggatggcg
      121 cgcatgaaga cggggcgcga aagcgtcatc accttcacgg gcggcttcca cgggcggacg
      181 gcggacgcca tcagcgcgac cttcctcggc aagtaccggg agatcgggcg accgaacgtg
      241 ccgggccatt tgtgcgcgga gttcggttcc atcgagtcgg tcgcggcgtt ggcggacgag
      301 acggtcgcgg cggtgatgct cgaaccgatt cagtcgatgg cgggtgtcgc cgtggccgcg
      361 ccggagttct acgtcgcgct gcggcggctg tgcgaggagc gcgggatgct cctcatttac
      421 gacgaggtgc agacgggcgt gggccgcacg ggcgagtggt tcttcgcggg gagcgaggcg
      481 tcgggcgcgg cggtgccgga catcatcacg ctggcgaagg cgctggggag cggggtgccg
      541 gtcggcgcgt gcctgacgac cgaggcggtg gccgccgcga tcaaagagaa cgatctggga
      601 acgaccttcg gcggggggat gctggcgatg gctgctgtca atgcgacgct cggtgcgatt
      661 gaagaggacg ggatgctgga gaacgcgcgc gcggtcgaag ctttcctgcg cgagagtctg
      721 gcgggcgtcg aaagggtcgc ggccctgcgc ggtcgcggcc tgctgctcgg cgtcgagttc
      781 atggagccga tagcgtcgga cgtgcagcgc gcgctgctca agcggcgcat catcaccggc
      841 acgtcgagcg acccgcgcgt gctgcgcctg ctgccgccgc tgtgcgtgag cgtcgaagaa
      901 gcgcggctgt tcgtcgaagc gctgggcgag gtgttggccg aaggatgacg agacactttc
      961 tgacgacggc ggactacacg cgcgaggagc ttgagggctt gctcgcggcg gcgctgcgtt
     1021 tcaagcgggg cgaggacagg tcgcggcctt tggcgggccg ctcggtcgcg ctcgtcttct
     1081 tcaacccttc tttgcggacg cgcgcgtcga tgcaggtcgg cgtgtacgag ctgggcggga
     1141 acgcggtcat cctggagccg ggcgggacga gctggacttt ggagcaccgc gacgggatag
     1201 tgatggacgg cgacaagacc gagcacgtcg cggagttcgt gcgcgtgctc gggcgctacg
     1261 cgtctctgat gggcgtgcgc acgttcgcgc agttgaagga ctgggaggag gagcggctcg
     1321 accccatcct caacgcgttc gcgaaatatt ccgaggtgcc ggtcgtcaac ctcgaatcgg
     1381 cgatgcacca cccgtgtcag gcgatggccg acatgctgac ggtgcgcgag aagctgggcg
     1441 aggggccgaa gcgcgtcgtg ctgacctggg cgtggcaccc gaagccgctg ccgatggccg
     1501 tgccgaacag cttcgcgctc gccgccgcgc agatgggtca cgagctgacc atcgcgcacc
     1561 cgcccggcta cgagctggac gaagagctga ttcaggacat caacctgcgc gccttcgacg
     1621 cgggcgcgaa ggtgacggtg acgaacgaca tcgaagaggc gttcgacggc gccgaggccg
     1681 tctacgcgaa gagctggggc gggcgcatgt tctacggcag cgcggcggaa gacatcgagg
     1741 agcgcgcgca gtaccgcgac cgctggatcg tggacgccgc caagatgcgg cgcaccgccg
     1801 gcggcatctt catgcactgc ctgcccgtgc gccgcaactg catcgtcacc gacgaggtga
     1861 tcgactcgtc ggcctcggtc gtcatcgacg aagcggagaa ccgtctgcac gtgcagaagg
     1921 caatcatcac aaaactactg tgaaggcaaa aggcaaaagg caaaaggcaa agggcaaaag
     1981 ttggaggcgc aacccgcttt taacttttgg tttttgcctt ttgccttttg ccttttgcct
     2041 tctttc
//
LOCUS       DBNGOH010000861         2742 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1413644, whole genome shotgun sequence.
ACCESSION   DBNGOH010000861 DBNGOH010000000
VERSION     DBNGOH010000861.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2742)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2742)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2742
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1413644"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2209
                     /gene="dnaX"
                     /locus_tag="AC159P_16630"
     CDS             <1..2209
                     /gene="dnaX"
                     /locus_tag="AC159P_16630"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR02397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HIR3697121.1"
                     /translation="LLKTLEEPPPRVLFIMATTHAHKVPETILSRSQQFEFRTIAAAK
                     IVERLHHAYLFSGARGVGKTTTARLLAKAVNCHRADKPTPSPCKTTDADQCPSCREVA
                     EGRSIDVLEIDAASNTGVDNVRDAIINTVGVRPARDRYKVFIIDEVHMLSVPAFNALL
                     KTLEEPPPRVLFIMATTHAHKVPETIISRSQQFEFRTIAAAKIAERLRLIADAEKIKV
                     GDDALREIARAGEGSMRDAQSAFDQVISFSEGEIETADVEAALGLAGTEMLARVMRSV
                     AAGAPAEALAVVDDLVVRGHDLRNFCRDLMGHLRDLLVTKVAGGASPLADGSDAERKA
                     LAAEADEFSESDLVRFFHSLTETEKNLREGAHPRYQLEIGLVKLVEMRRLAPLARVIE
                     RLGALEEALRTGKAPAATTTGGPGAAGPPGATPVAGGGNAPRRGSGAATGGGSAAPHT
                     PQAFNPPTAAAAKPSAAEVKSADSYSTPAPARTTTTTTATATARAATTTQAARPSAPV
                     ESPPPSATHAPNLKLVPAHTGNAADDLPPFPLDDEPSFTRGAPPFARDAPPEEPSYFA
                     DIPAGEDRAGPPVAFVPSVSPSSSSSSPSFGGAAEADDIVSRLKHALEARNKSLLVAA
                     LDAAQRVTFGDGVLCAEFAPEPKFMRDTLDKADSKCLLREVCAELTGRETEVRVVVHR
                     PGEVGAALTERDAALLEQKRLRERYENDPRVQPMLKTFRAEIVEVRRSDDEPR"
     gene            2392..2613
                     /locus_tag="AC159P_16635"
     CDS             2392..2613
                     /locus_tag="AC159P_16635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002619286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HIR3697122.1"
                     /translation="MRDPILAGILSLIVPGVGHLYNGQFLAGALWLMALVTIIPGFWI
                     GTGGILGLLCHVIAGYTAYNYAKEHRVRV"
BASE COUNT          441 a         1027 c          896 g          378 t
ORIGIN      
        1 gctcctcaag acgctggaag agccgccgcc gcgcgtcctc ttcatcatgg cgacgacgca
       61 cgcgcacaaa gtccccgaga cgatcctctc gcgcagccag cagttcgagt tccgcaccat
      121 cgccgccgcg aagatcgtcg agcgcctgca ccacgcctac ctcttctcgg gcgcgcgcgg
      181 cgtcggcaag acgacgaccg cgcggctgct cgccaaagcc gtcaactgcc accgcgccga
      241 caagccgacc ccttcgccgt gcaagacgac ggacgccgac caatgtcctt cgtgccgcga
      301 ggtcgccgag gggcgctcca tcgacgtgct cgaaatcgac gccgcgtcga acacgggcgt
      361 cgataatgtg cgcgacgcaa tcatcaacac cgtcggcgtc cggcccgcgc gcgaccgcta
      421 caaggtcttc atcatcgacg aagtgcacat gctgtcggtg cccgccttca acgcgctcct
      481 caagacgctc gaagagccgc cgccgcgcgt cctcttcatc atggcgacga cgcacgcgca
      541 caaagtcccg gagacgatca tctcgcgcag ccagcagttc gagttccgca ccatcgccgc
      601 cgcgaagatc gcggagcgcc tgcgcctcat cgccgacgcc gagaagataa aagtcggcga
      661 cgacgcgctc agggaaatcg cgcgcgcggg cgaaggctcg atgcgcgacg cgcagtcggc
      721 cttcgaccag gtcatcagct tcagcgaggg ggagatcgag accgccgatg tcgaggccgc
      781 gctcgggctg gccgggacgg agatgctggc gcgcgtgatg cgctcggtcg ccgcaggcgc
      841 accggcggag gcgctcgccg tcgtggacga cctcgtcgtg cgcggccacg acctgcgcaa
      901 cttctgccgc gacctgatgg gccacctgcg cgaccttttg gtgacgaagg tcgcgggcgg
      961 cgcgtcgccg ctcgccgacg gctcggacgc ggagcggaag gcgctcgccg ccgaggccga
     1021 cgaattctcc gagtccgacc tcgtgcgctt cttccacagc ctgacggaga cggagaagaa
     1081 tctgcgcgag ggcgcgcacc cgcgctacca gcttgaaatc ggcctggtga agctcgtcga
     1141 gatgcgacgg ctcgcgccgc tcgcgcgggt gatcgaacgc ctcggcgcgc tcgaagaggc
     1201 gctgcggacg ggcaaggcgc ccgccgccac gacaacgggc ggccccggcg cggcgggtcc
     1261 tccgggcgcg acgcccgtgg cgggcggcgg caacgctccg cgtcgcggca gcggcgccgc
     1321 gacgggcggc ggcagcgcgg ccccacacac cccgcaggct ttcaacccgc cgaccgccgc
     1381 ggcggccaaa ccctccgccg ccgaagtgaa gagcgcggac tcatattcga cgcccgcgcc
     1441 cgcccgcacg accacgacca cgaccgcgac cgcgaccgcg cgcgccgcga caaccactca
     1501 agccgcgcgt ccttcggcgc ccgtcgaaag ccctccgccg tccgcgacgc acgcgccgaa
     1561 ccttaaactc gtgccggcgc acacgggcaa cgcggcggac gacctgccgc cgttcccgct
     1621 cgacgacgaa ccttccttca cgcgcggcgc gccccccttc gcgcgcgacg cgccgccgga
     1681 agagccttcg tacttcgcgg acatccccgc cggcgaggat cgcgcggggc cgcccgtcgc
     1741 gttcgtcccg tcagtgtcac cttcatcttc gtcgtcttcg ccctcgttcg gcggcgcggc
     1801 tgaagcggac gacatcgtga gccgtctcaa gcacgcgctc gaagcgcgga acaagtcgct
     1861 tctcgtcgcg gcgctcgacg ccgcgcagcg cgtgacgttc ggcgacggcg tgttgtgcgc
     1921 cgagttcgcg cccgagccga agttcatgcg cgacaccctc gataaggctg acagcaaatg
     1981 cctcctgcgc gaggtctgcg ccgagctgac cgggcgcgag accgaggtgc gcgtcgtcgt
     2041 ccacaggccg ggcgaggtgg gcgccgccct cacggagcgc gacgcggcgc tgctcgaaca
     2101 gaagcgcctg cgcgagcgtt acgagaacga cccgcgggtg cagccgatgc tcaagacctt
     2161 ccgcgcggag atcgtcgagg tgcgccgctc cgacgacgag ccgcgctagg ccggaaggca
     2221 aaggctttgc ccgcgcgatt ggcggcagcc gaaagccctg ccccgcttga gggggcgcgt
     2281 ccttcgctcg gcctcggcgc gcgctccttg ctccgcttcg agtgcgcgtg ctctttcgac
     2341 gaacaaactc cgcgcgacgg gcgtataggc tcacggaggt gattcggaca catgcgcgac
     2401 cccatacttg caggcatact cagcttgatc gtccccggcg tggggcatct ctataacggc
     2461 cagtttctcg cgggcgccct ctggctgatg gccctcgtca ccatcatccc cggtttctgg
     2521 atcgggacgg gcggcatcct cggcctgctc tgccacgtca tcgcgggcta caccgcgtac
     2581 aactacgcga aagagcaccg cgtgcgagtc tgaccgcgaa cgttcccagc gcgcccgacc
     2641 cgaagcgagc ggaggcgaga ggttcgaacg acctctcgcc tctctccgct tgtagacccg
     2701 ccctcgcttc ggtcgggcat cgctcagccg taaaacattc cg
//
LOCUS       DBNGOH010000862         5392 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3063987, whole genome shotgun sequence.
ACCESSION   DBNGOH010000862 DBNGOH010000000
VERSION     DBNGOH010000862.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5392)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5392)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5392
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3063987"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2021
                     /locus_tag="AC159P_16640"
     CDS             <1..2021
                     /locus_tag="AC159P_16640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697123.1"
                     /translation="KDEDAQPSTPAGEDRSGDYTVISGIELGVRGLGVDGDHEKYRAD
                     LNYRPGFRLFDSSILVRAGDGDGPLFDNLVVNASGFGADPHGSLRVNADNGRWYKFDL
                     NYRRNRYVNRLITLARGDHYRNTTHNLGDFDLRLLPTNKRIRFNLGASFDRNGSEEPG
                     TTTYDALRDEFVVRHDYRTRAEEYRGGVEGRVGKLDLGFLVGTRGYRDDSTYFITQPE
                     AGSTAANRPAVLNTFRRDLPTHGRAWFTRLSAHTTIAERVDLTARYTYSHARTRFFLS
                     EVVTGINANRQTIERNVSEFAGESERPAHLFDFGVTWQPTDRLRISNTVRYNRFRNEG
                     AHVFSDINFLRTQAGAVVTPFPSTVTVPVARLLEYRRIHNQLKVDYQFGPRFSFHLGH
                     RVSDRDIEIDKRGNSVANNAAFAETESIETLNNSFFGGFKARPVKAWTLYFDFERGEA
                     DNTFTRVDNLDQISFRARSRVTPARGLGISLAFSTRDNSDPGLGVVDTTLVGPLDSSP
                     FSVEVDSRVFSTTVDWAPPSGRYTLNAGYTYTYLHSDAEILYFAGLTPNTIRFGRSLY
                     DVRDSFFFFNASARLHRLLSAYAGYRVHRDAGDRNETDAVGGLFARSYPLRFQSPEVR
                     LIFRLHRKVDWNVGYQYYEYKERLSDALNYRAHLPYTSLRFYWGGGER"
     gene            2053..3282
                     /locus_tag="AC159P_16645"
     CDS             2053..3282
                     /locus_tag="AC159P_16645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HIR3697124.1"
                     /translation="MRHGAVKLSIIFAVGTLFAVAARPGPAANGAAHEPVSQRRARTP
                     RRPPAPRPAYNDFSHATQEHRQSCDSCHKFPSRNWQEVRPKPDAFPDITQYPEHASCL
                     DCHRRQFFARERPVPQICSVCHVNATPRNTARHPFPTLGETYDTSTRGRTAVSDFAVN
                     FPHDKHEGLFAEHRFESRGVALFIRASFARARQDDPAKANAACATCHQTQQPQGDSAD
                     EFLTKPPKGLADGAYWPKKGTFKTTPRDHTTCFTCHSTDSGVAPLPTDCAACHKLLPP
                     ERRTLLAAAHDDFDPKLAAATGVNDRATLLKWARRGSAKFRHEWPPHDLACADCHAVA
                     TMNTADEATKKVRIVSCGGAGSGCHIEPTPDGILNTEVARKRADPSFDCTKCHVLNGR
                     RPLPDTHAAAITAATRK"
     gene            3346..4296
                     /locus_tag="AC159P_16650"
     CDS             3346..4296
                     /locus_tag="AC159P_16650"
                     /inference="COORDINATES: protein motif:HMM:NF025875.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HIR3697125.1"
                     /translation="MNETHEIDERRAAAGTAARARRRLSLVVVAAACLAFAPSLLPDR
                     GASASPTGAGWATHQLSPDGATNEVSNEPATAQGAVDYSKFRHTNPQHARLPCLLCHR
                     RESNSPRPVRSAGHTPCSGCHTQQFADASNPVCTICHANPSSGAVKPFPPLRTFNVRF
                     DHATHARPGARPAASCAACHQPTRRGVALSIPAGLAAHTTCYRCHAPRTQVAGRDLGS
                     CDNCHRVGGYRRTPESANAYRVGFSHAAHARAGASCNDCHTLRAGAAQGRQVTSPQPL
                     QHHAAPRAQSCMTCHDDRRAFGGDDFADCTRCHRGNAWHF"
     gene            4345..4917
                     /locus_tag="AC159P_16655"
     CDS             4345..4917
                     /locus_tag="AC159P_16655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HIR3697126.1"
                     /translation="MKLVITFALVGGLWLLGASSPPVPVAVGPPQGEKKPQDVYVFAA
                     ASKMGKVTFSHVDHITKNRNLDATGPVACVECHHTAQPASELAKLPPLKTAFPADRTT
                     TLTAELLAKDPNALGGVTCHKCHSPAGTKPEAWPELPEIKHETSTAVITLTNQQAFHR
                     NCAGCHDEVVKHRKDVKPPTSLKCAGCHKK"
     gene            4941..>5392
                     /locus_tag="AC159P_16660"
     CDS             4941..>5392
                     /locus_tag="AC159P_16660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697127.1"
                     /translation="MSEDKQPAPEETRPAAEETRPVQTTAGETRAAAPDAPSLFRNYV
                     SFTGAAIVAASLFSIVLLFLIEFTRSTDNPYLGIITYVVLPGFMAFGLAVVLFGMLRE
                     RRRRRHAPASLAAYPVLDFNDPRRRRNFFAFVGLAFVFIFMSAFGSYRA"
BASE COUNT          920 a         2161 c         1577 g          734 t
ORIGIN      
        1 cgaaggacga agacgcgcag ccctcgacgc ccgcgggcga agaccgttcg ggcgactaca
       61 ccgtcatctc cggcatcgag cttggcgtgc gcggcctcgg cgttgacggc gaccacgaga
      121 agtaccgcgc agacctcaac taccgccccg gcttccgcct cttcgattcg agcatcctcg
      181 tccgcgcagg cgacggcgac ggccccctct tcgacaacct cgtcgtcaac gcctcgggct
      241 tcggcgccga cccgcacggc tccttgcgcg tcaacgccga caacgggcgc tggtacaagt
      301 tcgacctcaa ctaccgccgc aaccgctacg tcaaccgcct catcacgctc gcgcgcggcg
      361 accactaccg caacacgacg cacaacctcg gcgacttcga cctgcgcctg ctccccacga
      421 acaaacggat tcgcttcaac ctcggcgctt cgttcgaccg caacggcagc gaggagccgg
      481 gcacgaccac ctacgacgcg ctgcgcgacg agttcgtcgt ccgccacgac taccgcacgc
      541 gcgcagagga gtaccgcggc ggcgtcgagg ggcgcgtcgg gaaactcgac ctcggcttcc
      601 tcgtcggcac gcgcggctac cgcgacgact cgacctactt catcacgcag cccgaagcgg
      661 gcagcacggc ggccaaccgc cccgccgtcc tcaacacctt ccgccgcgac ctgcccacgc
      721 acgggcgcgc ttggttcacg cgcctcagcg cgcacaccac catcgccgag cgcgtcgatc
      781 tcacggcccg ctacacctac tcgcacgcgc gcacgcgctt cttcctctcc gaggtcgtca
      841 cgggcatcaa cgccaaccgc cagaccatcg agcgcaacgt ctcggagttc gcgggcgagt
      901 ccgagcggcc cgcgcacctc ttcgacttcg gcgtgacgtg gcagccgacc gaccgtctgc
      961 ggatttcgaa caccgtccgc tataacaggt tccgcaacga gggcgcgcac gtcttcagcg
     1021 acatcaactt cctgcgcacg caggccgggg ccgtcgtcac gcccttcccc tcgaccgtca
     1081 ccgtgcccgt cgcgcggctc ctcgaatacc gccgcataca caaccagctc aaggtcgatt
     1141 accagttcgg cccacgcttc tctttccacc tcggccaccg cgtcagcgac cgcgacatcg
     1201 agatcgacaa gcgcggcaac agcgttgcga acaacgctgc cttcgccgaa accgagtcca
     1261 tcgagacgct caacaactct ttcttcggcg gcttcaaagc gcgccccgtc aaggcctgga
     1321 cgctctactt cgacttcgag cgcggcgagg ccgacaacac cttcacgcgc gtggacaacc
     1381 tcgaccagat atccttccgc gcgcgcagcc gcgtcacgcc cgcgcgcgga ctcggcatca
     1441 gcctcgcgtt ctcgacgagg gacaacagcg accccggcct cggcgtcgtg gacacgacgc
     1501 tcgtcggccc tctcgactcg tcgcccttca gcgtcgaagt ggacagccgc gtcttcagca
     1561 cgaccgtcga ttgggcgccg ccgagcgggc gctacaccct gaacgccggt tacacctaca
     1621 cctacctgca cagcgacgcc gagatactct acttcgccgg cctgacgccc aacaccatcc
     1681 gcttcgggcg cagcctctac gacgtgcgcg acagcttctt cttcttcaac gcgagcgcgc
     1741 gcctgcaccg gctgctctct gcctacgcgg gctaccgcgt ccaccgcgac gcgggcgacc
     1801 gaaacgagac cgacgccgtc ggcggcctct tcgcgcgctc gtacccgctg cgcttccagt
     1861 cgcccgaagt ccggctcatc ttccgcctgc accgcaaggt cgattggaac gtcggctacc
     1921 agtattacga gtacaaggaa cgtctctcgg acgcgctgaa ctaccgcgcg cacctgccct
     1981 acaccagctt gcgcttctac tggggcggcg gcgagcgtta gcgggcctcg gcccgccgga
     2041 cacgttgacg aagtgagaca cggggcagtc aagctatcga tcatcttcgc ggtcggcacg
     2101 ctcttcgccg tcgcagcgcg ccccggccct gcggcgaacg gcgcggcgca cgagcctgtc
     2161 tcgcaacgac gcgcccggac gccgcgccgc cctcccgccc cgcgccccgc ttacaacgac
     2221 ttctcgcacg ccacccaaga gcaccggcag tcctgcgact cgtgccacaa gttcccctcg
     2281 cggaactggc aggaggtgcg cccgaaaccg gacgccttcc ccgacatcac gcagtatccc
     2341 gagcacgcgt cctgcctcga ctgccaccgc cggcagttct tcgcgcgcga gcggcccgtc
     2401 ccgcaaatct gctccgtctg ccacgtcaac gcgacgcccc gcaacaccgc gcgccacccc
     2461 ttccccacgc tcggcgagac gtacgacacc tcgaccagag ggcgcaccgc cgtctcggac
     2521 ttcgccgtca acttcccaca cgacaaacac gaaggtctgt tcgccgagca ccgattcgaa
     2581 tcgagagggg tcgcgctctt cattcgcgcg tcgttcgcgc gcgcgcggca ggacgacccc
     2641 gcgaaggcca acgccgcctg cgcgacctgc caccagaccc aacagccgca gggcgattcc
     2701 gccgacgagt tcctgacgaa gccgcccaaa ggtctggccg acggcgccta ctggccgaag
     2761 aaaggcacct tcaagaccac gccgcgcgac cacacgacct gcttcacctg ccactcgacc
     2821 gactccggcg tcgcgcccct gccgacagac tgcgccgcct gccacaagct cctgccgccc
     2881 gaacggcgga cgctgctcgc cgccgcccac gacgacttcg acccgaagct cgccgccgcg
     2941 acgggcgtca acgatagggc cacgttgctc aagtgggcgc ggcgcggctc cgccaagttc
     3001 cgccacgagt ggccgccgca cgatctggcc tgcgccgact gccacgcggt cgccacgatg
     3061 aacaccgcgg acgaggcgac gaagaaagtg cgcatcgtct cctgcggcgg cgcaggctcc
     3121 ggctgccaca tcgagccgac gcccgacggc attctcaaca cggaggtcgc gcggaagcgt
     3181 gccgacccct ccttcgactg caccaagtgc catgtcctca acgggcggcg gcccttgccc
     3241 gacacgcacg ccgcggccat cacggcggcc acacggaagt gaacgcgacg aacgacacgc
     3301 ggcacaagca agcgagatga gcgacacgcg gaagcgccca acgccatgaa cgaaacgcac
     3361 gaaatcgacg agcggagagc ggcggcgggc acggcggcgc gcgcccgccg ccgtctctcg
     3421 ctcgtcgtcg tcgccgccgc ctgcctcgcc ttcgcgccgt cgctcctgcc cgaccgtggc
     3481 gcgtccgcct caccgacggg agcggggtgg gcgacccacc agttgtcgcc ggacggcgcg
     3541 acgaacgaag tctccaacga acccgcgacg gcgcagggcg cggtcgatta ctcgaagttc
     3601 cggcacacca acccgcagca cgcgcggctg ccgtgccttc tgtgccaccg ccgcgagagc
     3661 aactcgccgc gccccgtccg ctccgcaggc cacacgccct gctccggctg ccacacgcag
     3721 cagttcgcgg acgcgtcgaa ccccgtctgc acgatctgcc acgcgaaccc ttcgtcgggc
     3781 gcggtcaagc ccttcccccc tctgcgcacc ttcaacgtca ggttcgacca cgcgacgcac
     3841 gcgcgccccg gcgcgcgacc cgccgcgagc tgcgccgcct gccaccagcc gacgcggcgc
     3901 ggcgtcgcgc tctcgatccc cgcgggcctc gccgcgcaca cgacctgcta ccggtgccac
     3961 gcgccgcgca cacaggtcgc ggggcgcgac ctcggctcgt gcgacaactg ccaccgcgtc
     4021 ggcggctacc ggcgcacgcc cgagtcggcg aacgcctacc gcgtgggctt cagccacgcc
     4081 gcgcacgcgc gcgccggcgc gagctgcaac gactgtcaca cgctgcgggc cggcgcggcg
     4141 caaggccgac aggtcacttc cccgcagccg ctccagcacc acgccgcccc gcgcgcgcag
     4201 agctgcatga cctgccacga cgaccgccgc gcgttcggcg gcgacgactt cgccgactgc
     4261 accaggtgtc accgcggcaa cgcgtggcac ttctaagttc cttctcgaaa aggcaccacg
     4321 ttccacgata ggagggttac agggatgaaa ctcgttatca cgttcgcgct cgtcggtggc
     4381 ctgtggctgc tcggcgcctc ttcgcctccg gtgcccgtcg ccgtcgggcc tccgcagggc
     4441 gagaagaagc cgcaggatgt ctacgtcttc gccgcggcca gcaagatggg gaaggtcacg
     4501 ttcagccacg tcgatcacat cacgaagaac cgcaacctcg acgccacagg cccggtcgcc
     4561 tgcgtcgagt gccaccacac ggcgcagccc gcctccgagc tggcgaagct gccgccgctc
     4621 aagaccgcct tccccgccga ccgcacgacg acgctgacgg cggagctgct ggcgaaagac
     4681 ccgaacgcgc tcggcggcgt cacctgtcac aagtgccact cgcccgcggg caccaagccc
     4741 gaagcgtggc ccgagcttcc cgagatcaag cacgagacca gcaccgccgt catcacgctg
     4801 acgaaccagc aggcgttcca ccgcaactgc gccgggtgcc acgacgaggt ggtgaagcat
     4861 cgcaaggacg tgaagccgcc gacctctctg aagtgcgcgg gctgccacaa aaagtgaccg
     4921 cctgacgcga aggagcacac atgagcgagg acaaacaacc ggcacccgaa gagacgcgac
     4981 cggcagctga agagacgcga cccgtgcaga cgaccgcggg ggagacgcgc gcggcggccc
     5041 cggatgcgcc gagcctcttc cgcaactacg tcagcttcac gggcgcggcc atcgtcgccg
     5101 ccagcctctt cagcatcgtc ctgctcttcc tcatcgagtt cacgcgctcg accgacaacc
     5161 cctacctcgg catcatcacc tacgtcgtcc tccccggctt catggcgttc ggcctcgccg
     5221 tcgtcctctt cggcatgctc cgcgagcggc ggcggcgtcg ccacgcgccc gcctctctgg
     5281 cggcctaccc cgtccttgac ttcaacgacc cgcggcggcg gcgtaacttc ttcgccttcg
     5341 tcggcctcgc cttcgtcttc atcttcatga gcgccttcgg cagctaccgc gc
//
LOCUS       DBNGOH010000863         5323 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2329811, whole genome shotgun sequence.
ACCESSION   DBNGOH010000863 DBNGOH010000000
VERSION     DBNGOH010000863.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5323)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5323)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5323
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2329811"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..537)
                     /locus_tag="AC159P_16665"
     CDS             complement(<1..537)
                     /locus_tag="AC159P_16665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HIR3697128.1"
                     /translation="MDEKYFPEKIEEKWQRRWEESGAFEVEAGGAREAFYCLEMLPYP
                     SGFLHMGHVRNYSIGDALSWQRRLQGFNVLHPMGRDSFGQPAEQAAIKRGVMPRDWTE
                     ANIDHMRAQFKRMGFSFDWRREIAAHRPDYYKWDQWFFLKMLERGLAYRKKALVNWCP
                     QCATVLANEQVVDGCCWRH"
     gene            complement(857..1123)
                     /locus_tag="AC159P_16670"
     CDS             complement(857..1123)
                     /locus_tag="AC159P_16670"
                     /inference="COORDINATES: protein motif:HMM:NF012610.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIR3697129.1"
                     /translation="MARMDIVLKDRSEGPYYLQLRTEIETRIRDRRLAPGDALPSPAQ
                     LAQKLSIDKGEVQRAYFELEHAGLVARSVSKDFLGKEKVTYSVK"
     gene            complement(1123..2028)
                     /locus_tag="AC159P_16675"
     CDS             complement(1123..2028)
                     /locus_tag="AC159P_16675"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HIR3697130.1"
                     /translation="MKRRAARPSKVKAGPRPPIGFHAPIAGGLQNALLKARRLGADTV
                     QIFSRNPRGWQARPLTDEEVAAFEAVRHETAITPVVIHANYLINLAAAGEDIRAKSVA
                     SFREEVERGIRLGADYLVVHPGSARGACEADGIRTCAESLKAACDGLDLARFRVLLEN
                     TAGQGECIGHRFEHLREIIERCPELGLGVCLDTAHAFTAGYDLRDRDGFAATFKALER
                     NVGLENVRAVHFNDSRAAYNSRVDRHWHIGLGHIGAEALARVARHPGLAHAAFLLETP
                     QDELGDDERNLAALRSFVESNAAQA"
     gene            complement(2100..2561)
                     /locus_tag="AC159P_16680"
     CDS             complement(2100..2561)
                     /locus_tag="AC159P_16680"
                     /inference="COORDINATES: protein motif:HMM:NF013098.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR3697131.1"
                     /translation="MSASDFKVEQIDHVHVYVADRRAAAEWYGRVLGLVVLKEDETFD
                     EEDDGPVTVSSDGGSTSLALFRAPEGAGKFARRQTVAFRVGGAGFLEFLHRLELLDVA
                     GADGRRLGAGDVVDHDSSFSVYFKDPDGNPYELTTYDHDLVRRWLGASTGG"
     gene            complement(2661..3944)
                     /locus_tag="AC159P_16685"
     CDS             complement(2661..3944)
                     /locus_tag="AC159P_16685"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HIR3697132.1"
                     /translation="MNARKAFAAAVALAFALTLEGCRMPQNSGNSSANEPSQASMKTP
                     DTDAAPAAATDALSVHRRAVTIDLHADTVQFMVDEGADINRRLSTTHLDAVRMREGGL
                     DAQFFSIWVEPNLYGGGGARCVARADEQIAAVRALAERSPETWVLASTAADIRRAADE
                     GKLAALIGLEGGYAIDEKLENVERYYRMGVRYISPAWTYSLSWAGSSGDAAGRTRGLD
                     DFGRQVIREMNRLGIMVDVSHVSDKTFRDIVETATKPVIASHSNARAIVNHPRNLTDE
                     QIRALAKTGGVVCVVFYPAFVEPGWDESKARIDAEIAPLVRAAGEQATGRGSFRRIAR
                     DRVREREFKKRLPPVTVARIVDHIDHIVRLVGVAHVGLGSDFDGIQAVPADLSSVADF
                     PNLTEELLRRGYTEDDVLKILGGNILRVMEEVEKK"
     gene            4215..5303
                     /locus_tag="AC159P_16690"
     CDS             4215..5303
                     /locus_tag="AC159P_16690"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 -
                     branched-chain-amino-acid:2-oxoglutarate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HIR3697133.1"
                     /translation="MFNDDSRKKDVKTPAIVQEIWHNGEFIKWDDARVHVMTHVLHYG
                     SSVFEGIRCYKTERGPAVFRLHEHMQRLLNSAKIYRMEHEWTLDELCEAVVQLVARSN
                     FEKCYIRPIMFRSLDEEHPSFGVNPFPNPMACYIGAWDWGKYLGDAAIEQGVDVCVSS
                     WNRLTPNSMPAMAKAGSNYMNSQLIKMEAITNGYAEGIALDDRGYVSEGSGENIFLVH
                     EGKVITPPLASSILPGITRDSVIEICRALEIPLVESAIQRAALYVCDELFFTGTAAEV
                     TPIRSVDRIPVGAGARGPVTARIQDAFFKITSGERPAPGPWLTYVREHKAASGENNGH
                     KPEREIGKAASNAQAVDTREAIHVTLTE"
BASE COUNT          876 a         1812 c         1727 g          908 t
ORIGIN      
        1 gtggcgccag cagcacccat cgaccacctg ctcatttgcc agcaccgtcg cgcactgcgg
       61 acaccagttc accagcgcct tcttgcgata cgccagcccg cgctccaaca tcttcaggaa
      121 gaaccactga tcccacttgt aatagtcggg ccggtgcgcg gcgatctcgc gccgccagtc
      181 gaagctgaag cccatccgct tgaactgggc gcgcatgtgg tcgatgttcg cctccgtcca
      241 gtcgcggggc atcacgccgc gcttgatggc cgcctgttcg gcgggctggc cgaagctgtc
      301 cctgcccatc gggtgcagca cgttgaagcc ctgaaggcgc cgctgccacg acagcgcgtc
      361 gccgatggaa tagttacgca cgtgccccat gtgcagaaag ccggaagggt aggggagcat
      421 ctccagacaa taaaaagcct cgcgcgcgcc cccggcctcg acctcgaagg cgcccgactc
      481 ctcccagcgc cgctgccact tctcttcgat cttctcggga aaatattttt cgtccatgac
      541 tctctcgaca agcctttcct acctcgacgg taaacactac cagcccgcgc aaagctttct
      601 caagctaagc cgccgggcca tcgatgacaa gttgttcgag tgtcgctaaa ggtgtgtcgc
      661 cgcaccgccg ccgcgcgccc gatgggttca ggcgcgggcg ccgcggggcg gcaccgtaag
      721 gaggagtttc actggatggc cgaatcgttt catcttcgtc tttgacgccc tcgccttccg
      781 caaggacggc gtcatcttag cgcacgccgc gcgggcctgt ctaactttat ccgaacccgg
      841 cgcgcggccc gccccatcac ttgaccgagt aagtcacctt ctccttgccg agaaaatctt
      901 tgctcacgct gcgcgcgacc agccccgcgt gctcaagctc gaagtaggcg cgctgcactt
      961 cgcccttgtc gatggagagc ttctgcgcga gctgcgcggg cgaggggagc gcgtcgccgg
     1021 gggcgagtct gcggtcgcgg atgcgcgtct cgatctcggt gcggagctgt agatagtacg
     1081 gcccctcgga acggtctttg agcacgatgt ccatgcgcgc cattatgctt gagccgcatt
     1141 gctttcgaca aaagagcgga gcgcggcgag gttgcgctcg tcgtcgccca gctcgtcctg
     1201 cggcgtctca aggaggaagg cggcgtgcgc gagaccgggg tggcgcgcga cgcgcgctaa
     1261 ggcttcggcg ccgatgtggc cgaggccgat gtgccagtgc ctatcgacgc gcgagttgta
     1321 ggcggcgcgc gagtcgttga agtggacggc gcggacattc tccagcccga cgttgcgttc
     1381 gagcgctttg aaggtggcgg cgaagccgtc gcggtcgcgc aggtcgtagc cggcggtgaa
     1441 ggcgtgggcc gtgtcgaggc agacgccgag gccgagttcg gggcagcgct cgatgatctc
     1501 gcgcaggtgc tcgaagcggt ggccgatgca ctcgccctgc cccgcggtgt tttcgagcag
     1561 gacgcggaag cgcgcgaggt ccagcccgtc gcacgccgcc ttcaggctct cggcgcacgt
     1621 gcggatgccg tcggcctcgc acgcgccgcg cgcgctgccc ggatggacga cgaggtagtc
     1681 cgcgccgagc cgaatcccgc gctcgacctc ttcgcggaaa gaggcgaccg acttcgcgcg
     1741 gatgtcttcg ccggcggcgg cgaggttgat gaggtagttg gcgtggatga cgacgggcgt
     1801 gatggccgtc tcgtgccgga cggcttcgaa ggcggcgacc tcttcgtcgg tcaggggccg
     1861 cgcctgccag ccgcgcgggt tgcgcgagaa gatttgcact gtgtccgcgc cgaggcgacg
     1921 cgccttcagc agcgcgttct gcaacccgcc cgcgatgggc gcgtggaatc ctatcggtgg
     1981 gcgcggcccc gccttcactt tcgagggccg cgcggcgcgt cgcttcaaaa gggactccgt
     2041 tccggtcgtc gttgataaag agcgtcgggt tcagtcttcg gcatgaaagt cacttcgcgt
     2101 cagccgccgg tgctcgcgcc gagccaacgg cggacgaggt cgtggtcgta ggtcgtcagc
     2161 tcgtacgggt tgccgtccgg gtctttgaag tagacggaga aggaggagtc gtggtcaacc
     2221 acgtcgcccg cgccgaggcg ccgcccgtcg gcgcccgcga catcgagcaa ctcgaggcgg
     2281 tggaggaatt cgaggaagcc cgccccgccg acgcggaagg cgaccgtctg ccgccgcgcg
     2341 aatttgcccg cgccttccgg cgccctgaag agcgcgaggc tggtcgagcc gccgtcgctc
     2401 gaaaccgtca ccggcccatc gtcctcctcg tcgaaggtct cgtcttcctt caagaccaca
     2461 agcccgagga cgcgcccgta ccactccgcc gccgcgcgcc ggtcggcgac gtagacgtgg
     2521 acgtggtcga tctgctccac tttgaaatcg ctcgcgctca tgcccatctc ctacactcac
     2581 cgttagctgt aacgcatcaa cgcccgtgac gattgtaggg gcagggacaa gccctgcccc
     2641 tacagtcttg cggcgcgcgc ttattttttc tccacctctt ccatcacgcg caggatgttg
     2701 ccgccgagta tcttcagcac gtcgtcttcg gtgtagccgc gccgcagaag ctcctccgtc
     2761 agattcggga agtcggcgac cgacgagagg tctgcgggga cggcctgaat gccgtcgaag
     2821 tccgagccga ggccgacgtg tgcgacgccg acgagccgga cgatgtggtc gatgtgatcg
     2881 acgatgcgcg cgaccgtgac gggcggcaga cgtttcttga attcgcgctc gcgcacgcgg
     2941 tcgcgcgcga tgcgccggaa cgagccgcgg cccgtcgcct gctcgcccgc cgcgcgcacc
     3001 agcggcgcga tctccgcgtc gatgcgcgcc ttcgactcgt cccagcccgg ctcgacgaac
     3061 gccgggtaga agacgacgca cacgacgccg cccgtctttg ccaacgcgcg aatctgctcg
     3121 tccgtcaggt tgcgcgggtg gttcacgatg gcgcgcgcgt tcgagtgcga ggcgataacc
     3181 ggcttcgtcg cggtctcgac aatatcccgg aacgtcttgt cggagacgtg cgacacgtca
     3241 accatgatgc cgaggcggtt catctcgcgg atgacctgac gaccgaaatc gtcgaggccg
     3301 cgcgtgcgcc ccgccgcgtc gcccgaagag ccggcccagc tcaggctgta cgtccacgcg
     3361 gggctgatgt agcgcacgcc catccggtaa tagcgctcga cgttctccag cttctcgtct
     3421 atcgcatagc cgccttcgag gccgatgagc gccgcgagct tgccttcgtc cgccgcgcgg
     3481 cggatgtccg ctgcggtcga ggccagcacc cacgtctcgg ggctgcgctc ggcgagcgcg
     3541 cgcacggcgg cgatctgctc gtcggcccgc gcgacacagc gcgccccgcc gccgccgtag
     3601 aggttcggct cgacccagat cgagaagaac tgcgcgtcga gtccgccctc gcgcatcctg
     3661 acggcgtcga ggtgcgtcgt cgagagccgc cggttgatgt ccgctccctc gtccaccatg
     3721 aactgcacgg tgtcggcgtg gaggtcaatc gtgacggcgc ggcggtgaac cgaaagagcg
     3781 tcggtcgcgg cggcgggcgc ggcgtccgtg tccggagttt tcatcgaagc ttgcgacggc
     3841 tcgttcgcgc ttgagttccc actgttctgc ggcatgcggc atccttcgag cgtcagcgcg
     3901 aacgcgagcg cgacggcggc ggcgaacgcc ttgcgggcat tcaactgtgg cggagaaatg
     3961 gttaacaaag cgatttttta tcttgacgaa tgcgtgcacg atggtacgtc gcgcggtctg
     4021 gccgaatcaa gcgtgcggct gtagattcgc gccgcacgcg tcgaaagtgg tctgcttgcc
     4081 aagccgcgag cgtttacggt atcgtgctcc ttacttaacc agcaagcggt cgttcccgta
     4141 cctaatccgc cggagtgaat ttgccctcgc cgcccggcac cgcacaattc atcgagtaag
     4201 gagaagaggc catcatgttc aatgacgaca gcaggaagaa ggacgttaag acgccggcca
     4261 tcgtgcagga gatttggcac aacggcgagt tcatcaagtg ggacgacgcg cgcgtccacg
     4321 tgatgacgca cgtgctgcac tacggctcca gcgtcttcga gggcatccgc tgctacaaga
     4381 ccgagcgcgg ccccgccgtc ttccgcctgc acgaacacat gcagcgcctc ctcaactccg
     4441 ccaagatata ccgcatggag cacgagtgga cgctcgacga attgtgcgag gcggtcgtcc
     4501 agctcgtcgc gcgcagcaac ttcgagaagt gctacatccg ccccatcatg ttccgcagcc
     4561 tcgacgaaga gcacccgtcg ttcggcgtca acccgttccc gaacccgatg gcctgctaca
     4621 tcggcgcgtg ggactggggc aagtacctcg gcgacgcggc catcgagcag ggcgtggacg
     4681 tgtgcgtctc ctcttggaac cggctgacgc cgaactcgat gcccgcgatg gcgaaggccg
     4741 gctcgaacta catgaactcg cagctcatca agatggaggc catcaccaac ggctacgccg
     4801 agggcatcgc gctcgacgac cgcggctacg tctccgaagg cagcggcgag aacatcttcc
     4861 tcgtccacga aggcaaggtg attacgccgc cgctcgcctc ctccatcctg cccggcatca
     4921 cgcgcgacag cgtgatcgag atttgccgcg cgctggagat accgctcgtc gagtcggcca
     4981 tccagcgcgc cgcgctctac gtctgcgacg agctgttctt caccggcacc gccgccgagg
     5041 tcacgcccat ccgctcggtt gaccgcatcc cggtcggcgc tggcgcgcgc ggccccgtca
     5101 ccgcgcgcat ccaggacgcc ttcttcaaaa tcacctccgg cgagcgcccc gcgcccggcc
     5161 cctggctcac ctacgtccgc gagcacaagg ccgcctccgg cgagaacaac ggccacaagc
     5221 ccgaacgcga gatcggcaag gccgcctcca acgcgcaggc cgtggacacg cgcgaggcca
     5281 tccacgtcac gctcaccgaa taagtcgagc gtcggccacg cgg
//
LOCUS       DBNGOH010000864         4507 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2879660, whole genome shotgun sequence.
ACCESSION   DBNGOH010000864 DBNGOH010000000
VERSION     DBNGOH010000864.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4507)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4507)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4507
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2879660"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="AC159P_16695"
     CDS             <1..196
                     /locus_tag="AC159P_16695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697134.1"
                     /translation="DDGDERLDRAVRLLTAGGATLVACETERGTLLDALESYEREDQR
                     EGNGDGDDEDNAGGGQGVRS"
     gene            193..1023
                     /locus_tag="AC159P_16700"
     CDS             193..1023
                     /locus_tag="AC159P_16700"
                     /inference="COORDINATES: protein motif:HMM:NF013245.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3697135.1"
                     /translation="MRSLVLLFSRVRAFVVRDFQLAVSYRLEFFMRLVSILFVTTTLF
                     FISQIFAGRFDTPYEQWRDPFLAWITALPFLNYFMVGFSSLANAIRSEQAQGTLESVL
                     MTPINIPTLIVASSGWDFLLATFHSFLYLFFGWSLFGVQYRGNFLLALLFLVLTTLVL
                     ASIGILSASFAMVFKRGDPFGVIIGTGSALFSGVFFPTQLMSQVGGSGFGYVSRALPT
                     TYGIDGIRRVLIQGQGFAEARQPLLTLLVYLAVLLPFALWVFTRAVRRAKREGSLIQY
                     "
     gene            1218..3395
                     /locus_tag="AC159P_16705"
     CDS             1218..3395
                     /locus_tag="AC159P_16705"
                     /inference="COORDINATES: protein motif:HMM:NF025056.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HIR3697136.1"
                     /translation="MSQASLPSASKNPMAARHRKLSRLVLAAAITLAASPAPGAAPRP
                     LQTQKGQSLQAEQPKAEQPKVEPKPVEPLQQPGQTPDTGTVKQPPKYTVKITPRRAYV
                     MPCPGKPDSGKVTLRATINPKPPKPSKPTEYTWKGDKLSGTGNSITWDLSGLTPNTYT
                     ATVETPNPVPGLIVRLLSASVNVIVRPCSVPPPPKPPCPAPVVTCPEEVKAGERLICK
                     ATFTTTRDAPSYQEGSWSIFTDNGKKLFYTRLPDGQSVAVDTTGLGGRSVVATFGLRG
                     YEGICIGRGSASVVQPADNNNQPDGGGNSNQPDGTENDNQSGGGGNSNQPGETDTPVP
                     PATPVVAGTLAPFSSPSTSSTASTTPPGATSDSVYSPGVWGVSLDWRYLLLIAALLVL
                     TFFAFRWAMRDSSKPLDVRPAPKPLGSPDEPDESSDGRDKAFVAGGTKRVKDELQCTV
                     FAPFEAAPGDGFLVQAFAHLAEHARLLEGMAKEADRDAVRRGADKLGEVVRGQELFFH
                     LDMPGLAVDEPVQSVVWNGDIESVQFAVGVPDQFKPRRLNCKLTVSVEGIPIGHVKFV
                     FEVAAAPPPAGAETPLNSSGQFVRYRKGFVSYASEDLAEVLARVQVMTQLGIECFQDV
                     LSLRPGERWEKKLYLHIDESDVFFLFWSAAAHKSVWVEKEVRYALRRKSEDEDSPPEI
                     MPVILQGPPEVPPPSYLPDYHFNDPFALLIKAARTRRAEKEEG"
     gene            3518..4450
                     /locus_tag="AC159P_16710"
     CDS             3518..4450
                     /locus_tag="AC159P_16710"
                     /EC_number="3.5.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011404794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000118 - histone deacetylase complex
                     [Evidence IEA];
                     GO_function: GO:0004407 - histone deacetylase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HIR3697137.1"
                     /translation="MRVSYTPRYYADIGDGHVFPIRKFELVRAALLAEGTLAPADIFE
                     PRPAPVEDVLLVHTEDYVTRLRAGALTPREIRRLGLPWSKALVRRSFLATGGTLNAAR
                     WALEEGVGSNLAGGTHHAFPDHGEGFCVLNDVAVAVRVLRRDGLVRRAAIVDCDVHQG
                     NGTAAIFEGDPAVFTFSMHGAKNYPLFKTRSTLDVELDDGTGDEEYLCTLARHLARVL
                     EFTPDIVFYLGGADPYAGDKLGRLALSIEGLRARDEFVLAACRDRGVPVATVMSGGYA
                     KDISDTVEIHCNTVRAARSVFDGVQGSKFEVQSS"
BASE COUNT          768 a         1609 c         1384 g          746 t
ORIGIN      
        1 cgacgacggc gacgaacgtc tcgaccgcgc ggttcgctta ctgaccgccg ggggcgcgac
       61 gctcgtcgcc tgcgagaccg agcgaggcac gctgctcgac gcgctcgaaa gctacgagcg
      121 cgaagaccag cgcgaaggca acggcgacgg cgatgacgaa gacaacgcgg gcggcggtca
      181 gggggtgcga agttgagaag tctcgtgctg ctcttcagcc gcgtgcgcgc gttcgtcgtg
      241 cgcgacttcc agctcgccgt cagctaccgg ctggagttct tcatgcgcct cgtgagcatc
      301 ctcttcgtca cgacgacgct cttcttcatc tcgcagattt tcgcgggccg cttcgacacc
      361 ccttacgagc agtggcgcga ccccttcctc gcgtggatca cggcgctccc cttcctcaac
      421 tacttcatgg tcggcttctc cagcctcgcc aacgccatcc gctccgagca ggcgcagggg
      481 acactcgaaa gcgtgctgat gacgcccatc aacatcccga cgctgatcgt cgccagctcc
      541 ggttgggact tcctgctggc gacgttccac tcgttcctct acctcttctt cggctggtcg
      601 ctcttcggcg tccagtaccg cggcaacttc ctgctcgcgc tcctcttcct cgtcctgacg
      661 acgctcgtgc tcgcctccat cggcatcctc tcggcgagct tcgcgatggt cttcaagcgc
      721 ggcgatccgt tcggcgtcat catcggcaca ggctccgctc tcttctccgg ggtcttcttc
      781 ccgacgcagc tcatgtcgca ggtcggcggc tccggcttcg gctacgtctc gcgcgccctc
      841 cccaccacct acggcatcga cggcatccgc cgcgtcctca tccagggcca gggcttcgcc
      901 gaagcgcgcc agccgctgct cacgctcctc gtctacctcg ccgtcctgct ccccttcgcg
      961 ctctgggtct tcacccgcgc cgtccgccgc gccaaacgcg aaggcagcct cattcaatac
     1021 tagcggctca cgcgcgcggg cagcggacgc ggcgaaatac ttccgcccgt gacgcatcca
     1081 taattgaaca gcctcgcgcg gagattaggt ctttacgtgg ctcggggact agaatgcaat
     1141 ccgctcgccc gcatgaatcc tcatggcccc gcgtaggggt ctttgacccc gaccgagacc
     1201 acggagcgcg gcaaccgatg agccaagcct ccctcccatc agcctcgaag aacccgatgg
     1261 cggcccgcca ccgcaaactc tcacggctcg tcctcgccgc cgcgataaca ctcgccgcat
     1321 cccccgcgcc cggcgccgcc ccgcggcctc tacaaacaca gaagggacag tcgctacagg
     1381 cagagcagcc gaaggcagag cagccgaagg tggagccgaa gccggtggaa ccgctgcaac
     1441 agcccggaca gacaccggac accggcacgg taaagcagcc gccaaagtac acggtgaaga
     1501 tcacgccgcg cagggcttac gtcatgccct gccccggcaa acccgactcg gggaaggtga
     1561 cgctcagagc gacgattaac cccaaaccgc cgaagccctc gaagccgacg gagtacacgt
     1621 ggaaagggga taagctcagc ggcaccggaa attcgatcac gtgggacttg tccggcctga
     1681 cgccaaatac ttacacggcc accgtcgaga ccccgaaccc tgtgcccggc ctcatcgtca
     1741 ggttgctgag tgcctctgtt aatgtcatcg tcaggccgtg ctccgtaccg ccgccgccga
     1801 agccgccctg cccggcgcct gtcgtgacgt gcccggagga ggtcaaagcc ggagagcgtc
     1861 tgatctgcaa ggccacattc acgacgacgc gggacgcgcc gtcttatcaa gaaggctcgt
     1921 ggtctatctt caccgacaac ggcaagaaac tcttttacac acgcttgccc gacgggcaaa
     1981 gcgtcgcggt ggacacgacc ggcctgggcg gtaggtcagt cgtcgccaca ttcggtctca
     2041 gagggtatga agggatctgc atcggaaggg gcagcgcgtc cgtcgtccaa cccgcagaca
     2101 acaacaacca gcccgacggc gggggaaaca gcaaccagcc ggacggcaca gagaacgaca
     2161 accagtcggg aggaggagga aacagcaacc agccgggcga gacggacaca cccgtcccgc
     2221 ccgccacgcc cgtcgtggcc gggactctcg cccccttttc gtccccttca acttcttcga
     2281 ccgcgtcaac aacgccgccc ggcgctacgt ccgactcagt ctactcgccc ggcgtctggg
     2341 gcgtctctct cgactggcgt tacctgctcc tgatcgccgc cctcctcgtc ctgacgttct
     2401 tcgcgttccg ctgggcgatg cgcgactcgt cgaagccttt agacgtgcgc cccgccccca
     2461 aaccgttggg gtctcccgac gagccggatg agtcttccga cgggcgcgac aaagccttcg
     2521 tcgccggcgg cacgaagagg gtcaaggacg agttgcagtg caccgtcttc gcgccgttcg
     2581 aggccgcgcc cggcgacggc tttctggtgc aagccttcgc gcatctggcc gagcacgcgc
     2641 ggctgctgga agggatggcg aaggaggccg accgcgacgc cgtccgccgc ggcgcggata
     2701 agctcggcga ggtcgtgcgc ggtcaggagt tgttcttcca cctcgacatg cccggcctcg
     2761 cggtggacga acccgtgcag tccgtcgtct ggaacggcga catcgagtcg gtgcagttcg
     2821 ccgtaggcgt gcctgaccag ttcaagccgc ggcgcctcaa ctgcaagctc acggtcagcg
     2881 tcgagggcat ccccatcggg cacgtcaagt tcgtcttcga ggtcgccgcc gccccgccac
     2941 ccgcgggcgc cgagacgccc ttgaactcct ccggccaatt cgtccgctac cggaaaggct
     3001 tcgtctccta cgcctcggaa gatttggcgg aggtgctggc gcgcgtgcag gtgatgacgc
     3061 agctcggcat cgagtgcttt caggacgtgc tctcgctcag gccgggcgag cgctgggaga
     3121 agaagctcta cctgcacatc gacgagagtg acgtgttctt cctcttctgg tcggcggcgg
     3181 cgcacaagtc cgtgtgggtc gagaaggagg tgcgctacgc gctccgccgc aagagcgaag
     3241 acgaagacag cccgcccgag atcatgccgg tcatcctcca ggggccgccc gaagtgccgc
     3301 cgccgagcta cctgcccgac tatcacttca acgacccgtt cgcgctctta atcaaagcgg
     3361 ccagaacccg ccgggccgag aaggaggagg gctgaacggc cccgcgccgc tctgctacaa
     3421 tcgatttcgg attttggatt ttggatcgga gtgccgttta agccggacgc tggaggctca
     3481 gctctcgcgg cccgacaatc caaaatccga aatcgtcatg cgcgtctctt acaccccacg
     3541 ctactacgcg gacatcggcg acggccacgt cttcccgatt cggaagttcg agctggtgcg
     3601 cgcggcgctg ctcgcggaag gcacgctcgc gcccgccgac atcttcgagc cgcggcccgc
     3661 gcccgtggag gacgtgctgc tcgtgcacac ggaggactac gtgacgcgtc tgcgcgcggg
     3721 cgcgctcacg ccgcgcgaga ttcggcggct cggactgccg tggtcgaagg cgctcgtgcg
     3781 ccgttcgttt ctggcgacgg gcgggacgct gaacgccgcg cgctgggcgc tcgaagaggg
     3841 cgtcggctcg aacctcgcgg gcggcacgca ccacgccttc cccgaccacg gcgagggctt
     3901 ctgcgtgctc aacgacgtgg ccgtcgccgt ccgtgtgctc cgccgcgacg gactcgtcag
     3961 acgcgcggcc atcgtcgatt gcgacgtgca tcagggcaac ggcaccgccg ccatcttcga
     4021 gggcgacccc gcggtcttca ccttctcgat gcacggcgcg aagaactacc cgctcttcaa
     4081 gacgcgctcg acgctcgacg tggaactcga cgacgggacg ggcgacgaag aatacctctg
     4141 cacgctcgcg cgccacctcg cgcgcgtcct cgagttcacg cccgacatcg tcttctacct
     4201 cggcggcgcg gacccttacg cgggcgacaa gctcgggcgc ctcgcgctct ccatcgaggg
     4261 gctgcgcgcc cgcgacgagt tcgtgctcgc ggcctgccgc gaccgcggcg tgcccgtcgc
     4321 caccgtcatg tccggcggct acgccaaaga catctcggac accgtcgaga tacactgcaa
     4381 caccgtccgc gccgcgcgct ccgtcttcga cggggttcaa ggttcgaagt tcgaggttca
     4441 aagctcgtag gacgaatgat gaagatcgtg ttggcatcac tgtaggggca gggcaagtcc
     4501 gcggccc
//
LOCUS       DBNGOH010000865         8197 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4162220, whole genome shotgun sequence.
ACCESSION   DBNGOH010000865 DBNGOH010000000
VERSION     DBNGOH010000865.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8197)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8197)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8197
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4162220"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1698)
                     /locus_tag="AC159P_16715"
     CDS             complement(<1..1698)
                     /locus_tag="AC159P_16715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018477779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR3697138.1"
                     /translation="MRRSTARAAAPDDDFTHRGGFEMETDSGRSTSVWMATAEVPAAS
                     PLQEDARCEVCVVGAGIAGMTTAYLLAREGKSVVVIDDGPVAGGETSRTTAHLTNALD
                     DRYYELERLHGERGARLAAESHTRAIDLIEEIVEEEGIDCDFARLDGYLFVPPGDPTD
                     QLGEELRAAHRAGLTEVEYVERVPFDAYDFGAALRFPYQGQFHILKYINGLTEAFRRR
                     GGRVHTKTHAAEIVGGDAAHVKTADGFRVTASSIVVATNSPVNDLVAIHTKQAAYRSY
                     VVGARVPRGSVPKILLWDTPDPYHYVRIQSVEGAGAGDAHDVLIVGGEDHKTGQAEDF
                     EERYRRLEEWTRERFPAVESIEFRWSGQVLEPVDGLAFIGRNPLDSENVYIATGDSGN
                     GMTHGTIAGILITDLIAGRASEWATLYDPSRKTLRAGGEFLKENLNVAAQYADLVTGG
                     EVDSIEDVRPGTGAVLRRGLKKIAVYRDDEGHVRELSAVCTHLGCIVDWNDTEKSWDC
                     PCHGSRFDPRDGQIGLEECASFRDGVDSPGIVDVGPRHGPAGDERGEEPSGHHAVAHA
                     "
     gene            complement(1823..2116)
                     /locus_tag="AC159P_16720"
     CDS             complement(1823..2116)
                     /locus_tag="AC159P_16720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015104411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HIR3697139.1"
                     /translation="MLAILGWIIFGLIAGVIAKAIMPGKDPGGAIITILLGIVGAIIG
                     GFVGRTLFGYGARVNDAGDLSQPGFLMSLVLAVVGAIIVLAVYRLITRRSIRA"
     gene            2465..3034
                     /locus_tag="AC159P_16725"
     CDS             2465..3034
                     /locus_tag="AC159P_16725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020655188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697140.1"
                     /translation="MNQIGELLQQYNGADPRHAPDTVENDFDQLAQNAPPNELADGLA
                     AAFRSDQTPAFGQMVSQMFANADGQQRASLLNTLLRTVGPTILAQVLSRGGGGGGLAG
                     LLGGGGGGGGLAGLLGGGAAPEVTPEQAEQVSPEEVAQIAEQAEQQDPSIIDRVSDFY
                     SRQPTLVKTLGAAALTIALAKIADRQYRG"
     gene            complement(3242..3850)
                     /locus_tag="AC159P_16730"
     CDS             complement(3242..3850)
                     /locus_tag="AC159P_16730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697141.1"
                     /translation="MAKKGGHDESRAAEGEPSKQELQQQMEATRDSISETVAEIREVV
                     SQQYDEVKETYESVKEGVDEALDWREKFSENPVVWGAGAVSVGILIGIGLAQTFEDED
                     ARGSRRKSKAAGAAGYLVGELSGFADAVLPTISGKVKELFGLDLAAYLGEARERARLK
                     GATERGARKKSAAKRSSKGATKKRAASKKGGAAKRGASKKRA"
     gene            complement(3843..4268)
                     /locus_tag="AC159P_16735"
     CDS             complement(3843..4268)
                     /locus_tag="AC159P_16735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012525770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3037 domain-containing protein"
                     /protein_id="HIR3697142.1"
                     /translation="MPDLCPFDYAVIRVVPRVERGEFVNAGVIVSCPSKNYLKARIEL
                     DRARLAALDPLVDAATIERHLDAVAAICEGGEGAGPIGLLPPRARFHWLVAPRSTVIQ
                     TSPVHTGLCRDGEEMLEHLLDTMVRPPAGARREGAKTNG"
     gene            complement(4246..5031)
                     /locus_tag="AC159P_16740"
     CDS             complement(4246..5031)
                     /locus_tag="AC159P_16740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006633038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA family kinase"
                     /protein_id="HIR3697143.1"
                     /translation="MNLRTVHATRYVTPLREGGSLPAIVEADDDGLYVLKFRGAGQGP
                     KALVAELVVGELGRVLGLPVPEIVLVELDPELARTEPDPEIQDLIRASAGLNLALDYL
                     PGAIAFDPLVDKPDAALASAVVWLDAYTTNVDRTPRNTNLLTWHRRLYLIDHGAALYF
                     HHDWPNYTRERARRPFAQIREHVLLPLAGALGEADADLSARVTPELVREVLSSVPDGW
                     LAAEPRFADAGEHRAAYADYLLTRLAPPRDFVEEAAHARSLSV"
     gene            5207..6274
                     /locus_tag="AC159P_16745"
     CDS             5207..6274
                     /locus_tag="AC159P_16745"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008892843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR3697144.1"
                     /translation="MNVKLKNLSEQVMVITGATSGIGLTTARAAAKRGVRLVLAARNE
                     DALREIVEEIKGAGGNAVYAVADVGREDDVRRIAETAEQHFGGFDTWVNNAGVSIFGN
                     MLEVSNEDHRQLFETNFWGVVYGSQVAARQLKQRGGGALINVGSTLSDRAIPVQGMYS
                     ATKHAVKGFTDALRMELEREGAPVSVTLVKPSAINTPFTRHAKNYMEHEPSLPPPVYA
                     PEVVAEVILHCAETPVRDAFAGGGGKALSASGHYAPRVTDKLMEATMFDLQRSDRPRL
                     ADRPDSLHAPSRDGDERGDYEGHVAESSAYNKASLHPLLTGALIAGAGLALAAWWRPG
                     WRGNGHGNGGRKLRTPNEGGH"
     gene            6400..7287
                     /locus_tag="AC159P_16750"
     CDS             6400..7287
                     /locus_tag="AC159P_16750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697145.1"
                     /translation="MFPFALFIVGSKLLHKGKRLAPACALFVLLGAAVHTPRATQRGA
                     RGGPAAAARTNGVPRLRPSNILDDLRKRAEGQPRPAPRELAAHANDLLARKGFDYRFD
                     ACEMFASAKPVERDETEQPATVVYPYRLTREDGGGPILFHLVGEADGSMCGECYFPLP
                     ALRVTKREMFVVADGVRYRLRRPTGFMLDEAHLVDSTLRRVLRTWQMPFQTIPAGISP
                     DGKLLYLSFYDDTGLGDLVLEVSEGGTPRFAVRKDVIEGEGEWIEDHPKDPKNAYLSF
                     MRFRSGGRSHVVRFSGPCT"
     gene            7295..>8197
                     /locus_tag="AC159P_16755"
     CDS             7295..>8197
                     /locus_tag="AC159P_16755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HIR3697146.1"
                     /translation="MTTTYKIALASLLLLQAAMSQTSSEKTRPRARDIGMVVGVLPPG
                     RLNAITDVEGVRVGHETLMKGDAVRTGVTAIVPHGGNLFQEKVPAAVFVGNGFGKLAG
                     STQVEELGEIETPILLTNTLSVPRAADALVEWMLALPGNEQVRSVNPLVGETNDGFLN
                     DIRGRHVGREEVFRALRAARGGAVEEGAVGAGTGTVAFGFKGGIGTSSRVLPASRGGY
                     TVGVLVQTNFGGVLTMNGAPVGRELGRYYLREDLETAPRAGGAEGQDSAAPPLGSPAA
                     DRADGSVIIVVATDAPLDARNLRRL"
BASE COUNT         1298 a         2862 c         2742 g         1295 t
ORIGIN      
        1 ggcatgagca acggcgtgat ggccggacgg ctcctcaccg cgctcatcac cggccggacc
       61 atgccgtggg ccgacatcta cgatccccgg cgaatccacc ccatcgcgga agctggcaca
      121 ttcctcaagg ccaatctggc cgtcgcgcgg gtcgaagcgc gagccgtggc aggggcagtc
      181 ccaggacttc tccgtgtcgt tccaatcgac gatgcagccg aggtgcgtgc agacggcgga
      241 gagttcgcgg acgtggcctt cgtcgtcgcg gtagacggcg atcttcttga ggccgcggcg
      301 cagcacggcg ccggtgccgg ggcggacatc ttcgatcgaa tcgacctcgc cgcccgtgac
      361 gaggtctgcg tactgcgcgg cgacgttgag gttctctttc aggaactcgc cgcccgcgcg
      421 caaggtcttg cgcgaagggt cgtagagcgt ggcccactcg ctcgcgcggc ccgcgatgag
      481 gtctgtgatg aggatgccgg cgatggtgcc gtgcgtcatg ccgttgccgg agtcgccggt
      541 ggcgatgtag acgttctcgc tgtcgagcgg gttgcgaccg atgaaggcca ggccgtccac
      601 gggttcgagc acctgacccg accagcggaa ctctatcgac tcgaccgcgg ggaagcgctc
      661 gcgcgtccac tcttccagac ggcggtaacg ctcctcgaag tcttcggcct gaccggtctt
      721 gtgatcctcg ccgccgacga tcagcacgtc gtgggcgtcg cccgcccccg cgccttcgac
      781 cgactggatg cggacgtagt ggtaagggtc gggcgtgtcc cacaaaagaa tcttcggcac
      841 cgagccgcgg ggcacgcgcg cgccgacgac gtaggagcga taggccgcct gcttggtgtg
      901 gatggcgaca aggtcgttga ccggcgagtt ggtggcgacg acgattgacg aggccgtcac
      961 gcggaagccg tcggcggtct tgacgtgcgc cgcgtcgccg ccgacgatct cggcggcgtg
     1021 cgtcttcgtg tggacgcgac cgccgcgccg ccggaacgcc tcggtgagtc cgttgatgta
     1081 cttcaggatg tgaaactggc cttgataagg gaagcggagc gcggcgccga agtcgtaagc
     1141 gtcgaaaggc acgcgctcga cgtactcgac ctcggtcagc ccggcgcggt gcgcggcgcg
     1201 cagctcctcg ccgagctggt cggtcgggtc gcccggcggc acgaagaggt agccgtcgag
     1261 gcgcgcgaag tcgcagtcga tgccttcttc ctcgacgatc tcttcgatga ggtcgatggc
     1321 gcgcgtgtgg ctctctgcgg cgagacgcgc gccgcgctcg ccgtgcaggc gttcgagttc
     1381 gtagtagcgg tcgtcgagcg cgttggtcag gtgcgccgtc gtgcgcgagg tctcgccccc
     1441 tgcgacgggg ccgtcgtcga tgacgaccac cgacttgccc tcgcgcgcga gcaggtaggc
     1501 ggtcgtcatc cccgcgatgc ctgcgccgac gacgcacacc tcacagcgcg cgtcctcctg
     1561 aagcggcgag gccgcgggca cctcggcggt cgccatccac accgaagtgc ttcggcccga
     1621 atcggtctcc atctcgaacc ctcccctgtg tgtgaaatcg tcgtccggcg ccgcggcgcg
     1681 cgcggtcgag cggcgcacga cgagtgcgcg gacgagctgt cgccgccgcg ggcgagaaag
     1741 accgccgcca cacctcgcgg cacgaaagcg cgagggcggc ggcggtcttc gagttgttag
     1801 tggtcggact tgcccgcgcc tgttacgcgc gtatgctgcg gcgcgtgatg agcctgtaaa
     1861 cggcgagcac gatgatcgcg ccgacgaccg cgaggacgag gctcatcaaa aagccgggtt
     1921 ggctcaggtc gccagcgtcg ttcacacggg caccgtagcc gaacagcgtg cgcccgacga
     1981 agccgccgat gatcgcgccg actatgccga gcaggatcgt gataatcgcg ccgccggggt
     2041 ctttgcccgg catgatggcc ttcgcaatca cacccgcgat caagccgaaa ataatccatc
     2101 cgagaatcgc cagcatcgtt cagttcctcc tcaagggaat gtttgcaata ctattgccct
     2161 acacccgccg gacgacagtg cgtactgttt ccgtatcgga tgaaggccgt cccgcgaggt
     2221 aagtgcaaac ggcgggccgc ggcaaaaact tcccgtcgcc gccgaaatca gccgaaccgc
     2281 cgcgcgacgc cgccggacag acggcggaag ggcaggcggc atgaacgttg ccaacgcaca
     2341 tgacgcgtgg gccgtccctt cggtgtcagc atcggaaccg cgacggcgtg tgccgcgtct
     2401 tctatcaacg tgcgcggcga gagtcaagta tctttttttt cggaggacta aacttgtgaa
     2461 ctggatgaat cagatcggcg agctgttgca gcagtacaac ggggccgacc cgcgccacgc
     2521 gcccgacacc gtggagaacg acttcgacca actggcgcag aacgcgccgc ccaacgaact
     2581 cgccgacggc ctcgcggcgg ccttccgctc cgaccagacg cccgccttcg ggcagatggt
     2641 ctcgcagatg ttcgccaacg ccgacggcca gcagcgcgcg agcctgctca acacgctgct
     2701 ccgcacggtc ggcccgacca tcctcgcgca ggtgctctcg cgcggaggag gcgggggcgg
     2761 cctcgccggt ctcctcgggg gcggtggcgg cggcggcggt ctcgccggat tgctcggcgg
     2821 cggcgcggcg cccgaggtga cgcccgagca ggccgagcag gtctcgcccg aagaggtcgc
     2881 gcagatcgcc gaacaggccg agcagcaaga cccctcgatc atcgaccggg tcagcgactt
     2941 ctactcaaga cagcccacgc tcgtcaagac gctcggtgcg gcggcgctca ccatcgccct
     3001 cgccaagatc gccgaccgtc agtacagggg ctagagcttc cgcgccgcgg agcaagggtc
     3061 atgcgaccat gcaccgtgcg tgataattgt aggggtaggg cttgtcctca gcccgttgct
     3121 tcgacgaatt caatcttgaa tcgattcgtc ggcggcgggc agggacaagc cctgccccta
     3181 caggctcaac ggacgcctta cactcttctt gaaactgctc ggcattcccg gcggtcaatc
     3241 atcacgccct ctttttactc gcccctcgct tcgccgcgcc gcccttcttc gacgccgcgc
     3301 gcttcttcgt cgcgcccttg ctcgaacgct tcgccgcgct ctttttacgc gcgcctcttt
     3361 cagtcgcgcc cttcagccgc gcgcgttcgc gcgcctcgcc gaggtaggcc gcgaggtcaa
     3421 ggccgaacag ctctttgacc ttgccggaga tggtcggcag cactgcgtcc gcgaaacccg
     3481 acaactcgcc gaccagatac ccggcggcgc ccgccgcctt cgacttgcgg cgagagccgc
     3541 gcgcgtcttc gtcttcgaac gtctgcgcca ggccgatgcc gatgaggatg ccgacggaga
     3601 ccgcgcccgc gccccacacg accgggttct cgctgaactt ctcgcgccag tcgagcgcct
     3661 cgtccacgcc ctccttgacc gactcgtagg tctccttcac ttcatcgtac tgctgcgaga
     3721 cgacctccct gatctccgcg accgtctcgc tgatcgaatc gcgcgtcgcc tccatctgtt
     3781 gctgaagctc ctgcttcgaa ggctctccct ccgccgcgcg agattcgtcg tgtccgccct
     3841 tcttagccat tggtcttcgc tccttcgcgg cgcgcgccgg caggcgggcg caccatcgtg
     3901 tccagcaggt gttcgagcat ctcttcgccg tcgcggcaga ggcccgtgtg gacgggcgag
     3961 gtctggatga ccgtgctgcg cggggcgacc agccagtgga agcgtgcgcg cggcgggagc
     4021 agcccgatgg ggccggcccc ttcgccgccc tcgcagatcg cggccacggc gtcgaggtgc
     4081 cgctcgatcg tcgccgcgtc cacgagcggg tcgagcgcgg cgagccgggc gcgatccagc
     4141 tcgatccgcg ccttcaggta gttcttcgac gggcacgaga cgatcacgcc cgcgttgacg
     4201 aactcgcccc gctcgacgcg cgggacgacg cggatgacgg cgtaatcaaa cggacagaga
     4261 tcgggcatgg gccgcctcct ctacgaagtc gcgcgggggc gcgagccgcg tcagcaggta
     4321 gtccgcgtac gcggccctgt gctcgcccgc gtccgcgaac cgcggctcgg cggcgagcca
     4381 cccgtcgggg acagagctta agacctcgcg gacgagttcg ggcgtcacgc gcgcgctcaa
     4441 gtccgcgtcc gcctcgccga gcgcgccggc cagcgggagc aggacgtgct ctctgatctg
     4501 cgcgaacggg cggcgggcgc gctccctcgt gtaattcggc cagtcgtggt gaaagtagag
     4561 cgccgcgccg tggtcgatca ggtagagccg ccggtgccac gtcagcaggt tggtgttgcg
     4621 cggcgtgcgg tcaacgttcg tcgtgtaggc gtcgagccag acgaccgccg acgccagcgc
     4681 ggcgtcgggt ttgtccacga gcgggtcgaa agcaatcgcg cccggcagat agtcgagcgc
     4741 gaggttgagg cccgcgctcg cgcggatcaa gtcctgtatc tccgggtcgg gttccgtgcg
     4801 cgccagctcc gggtcgagtt cgaccagcac gatctccggc accggcaggc cgagcacgcg
     4861 cccgagttcg ccgacgacca gttcggcgac gagcgccttc ggcccctgcc ccgccccgcg
     4921 gaacttgagc acgtagaggc cgtcgtcgtc cgcctccacg atggccggca gcgacccgcc
     4981 ctcgcgcagc ggcgtgacgt acctcgtcgc gtggacggtt ctcaggttca tcccgagagg
     5041 atacgcgcga cacggcctcg cccgtcaagt ctgccccgcg ccgaccggcg ctttacacgt
     5101 ggcggggcag acactatgct aaggcccgtt ccccttcaca acatcccccg cggtttcgag
     5161 cctcccccgg ccatcatcca tcaatcgacg aggagggtct attttcgtga acgtcaagtt
     5221 gaagaatctg agtgaacagg tcatggtcat caccggcgcc acgtcaggca tcggcctgac
     5281 gacggcgcgc gcggcggcca agcgcggcgt acggctggtg ctcgcggcgc gcaacgaaga
     5341 cgcgctgcgc gagatagtag aagagatcaa aggcgcgggc ggcaacgccg tctacgccgt
     5401 ggccgacgtg gggcgcgaag atgacgtgcg gcgcatcgcc gagacggcgg agcagcactt
     5461 cggcggcttc gacacgtggg tcaacaacgc gggcgtctcc atcttcggca atatgctcga
     5521 agtctcgaac gaagaccacc gccagctttt cgagacgaac ttctggggcg tcgtctacgg
     5581 ctcgcaggtc gccgcgcgcc aactgaagca gcggggcggc ggcgcgctca tcaacgtcgg
     5641 cagcacgctc tccgaccgcg ccatccccgt gcagggcatg tacagcgcga cgaagcacgc
     5701 cgtcaaaggt ttcaccgacg cgctccggat ggaactcgaa cgcgagggcg cgcccgtctc
     5761 cgtcacgctc gtcaagccct ccgccatcaa cacgcccttc acgcgccacg ccaagaacta
     5821 catggagcac gaaccttcgc tcccgccgcc cgtctacgcg cccgaggtgg tggccgaggt
     5881 gatactgcac tgcgccgaga cgcccgtgcg cgacgcgttc gcgggcggcg gcggcaaggc
     5941 gctgtcggcc tccggccact acgcgccgcg cgtcaccgac aagctcatgg aggcgacgat
     6001 gttcgacctc cagcgctcgg atcgtccccg cctcgcggac aggcccgaca gcctccacgc
     6061 gccttcgcgg gacggcgacg agcgcggcga ctacgaaggc cacgtcgccg agtcgtccgc
     6121 ctacaacaag gcttcgctcc acccgctcct caccggcgcg ctcatcgcgg gcgcagggtt
     6181 agcgctcgcc gcgtggtggc gccccggctg gcgcggcaac ggccacggca acggcggtcg
     6241 caagcttcgc acgccgaacg agggcggcca ctgattcgtc tctccgttcg agcgctcgcg
     6301 tgaaaggcaa ggtcgaagaa aggggcaagc ccttcgtctt cgaccttgcc ttttaccttt
     6361 tccctttcac ctttccggcg ggtgctatat tctcacgcca tgttcccgtt cgcgctgttc
     6421 atcgtcggct cgaaactcct tcacaagggc aagagactgg cgcccgcgtg cgcgttgttc
     6481 gtgctgctcg gcgcggcggt gcacacgccg cgggccacgc agcgcggggc gcgcggcggc
     6541 cccgccgcgg ctgcacgcac gaacggcgtc ccgcgtctgc gcccatcgaa cattctggac
     6601 gacttgcgga agagggccga ggggcagccg cgccccgcgc cccgcgagct ggccgcgcac
     6661 gccaacgacc tgctcgcccg gaagggcttc gactaccgct tcgacgcgtg cgagatgttc
     6721 gcgtccgcga agcccgtcga gcgtgacgag acggagcagc cggcgacggt cgtctacccg
     6781 taccggctga cgcgcgagga cggcggcggc cccatcctct tccacctcgt cggcgaagct
     6841 gacggcagca tgtgcgggga atgttacttt ccgctcccgg ccctgcgcgt gacgaagcgc
     6901 gagatgttcg tcgtcgcgga cggcgtgcgc tatcggctgc ggcgtcccac aggtttcatg
     6961 ctcgacgagg cgcatctggt tgacagcacg ctgcggcgcg tcttgcggac atggcagatg
     7021 cccttccaga ccatccccgc cggcatctcg ccggacggca aacttttgta cctcagcttc
     7081 tacgacgaca cggggttggg cgacctcgtg ctggaagtct cggaaggcgg gacgccccgc
     7141 ttcgcggtca ggaaggacgt gatcgaaggc gaaggcgagt ggatcgaaga ccacccgaaa
     7201 gacccgaaga acgcctacct ctccttcatg cgcttccgct ccggcggcag gagccacgtc
     7261 gtccggttca gcgggccgtg cacttgaaga gaagatgacg accacttaca aaatcgccct
     7321 cgcctccctg ctcctcctac aggccgccat gagtcagaca agcagcgaga agacgcgccc
     7381 gcgggcgcgc gacatcggga tggtggtcgg cgtgctgccg cccggcaggc tcaacgcgat
     7441 cacggacgta gagggcgtgc gcgtcggcca cgagactttg atgaaaggcg acgcggtgcg
     7501 aacgggcgtg acggccatcg tgccgcacgg cggcaacctc tttcaggaga aggtgcccgc
     7561 cgccgtcttc gtcggcaacg gcttcggcaa gctcgccggc tcgacgcagg tcgaagaact
     7621 cggcgagatc gagaccccga tactgctcac caacacgctc agcgtcccgc gcgcggctga
     7681 cgcgctcgtc gagtggatgc tcgccctgcc cggcaacgag caggtgcgct ccgtcaaccc
     7741 gctcgtcggc gagacgaacg acggcttcct caacgacata cggggccgcc acgtcgggcg
     7801 cgaagaggtc ttccgcgcgc tgcgtgcggc gcggggcggc gcggtcgaag agggcgcggt
     7861 cggcgcgggc acgggcaccg tcgccttcgg cttcaagggc ggcatcggca cctcttcgcg
     7921 cgtcctgccc gcaagccgcg gcggctacac cgtcggcgtg ctcgtgcaga ccaacttcgg
     7981 cggcgtcctc acgatgaacg gcgcgcccgt ggggcgcgaa ctcggcaggt attatctgcg
     8041 ggaagacctc gaaactgcgc cgcgcgccgg gggcgccgag gggcaggact ccgccgcgcc
     8101 gcctctcggt tcacccgcgg ccgaccgcgc cgacggttcc gtcatcatcg tcgtggcgac
     8161 ggacgccccg ctcgatgcgc gcaatctgcg ccgcctc
//
LOCUS       DBNGOH010000866         5231 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3429982, whole genome shotgun sequence.
ACCESSION   DBNGOH010000866 DBNGOH010000000
VERSION     DBNGOH010000866.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5231)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5231)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5231
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3429982"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1295)
                     /locus_tag="AC159P_16760"
     CDS             complement(<1..1295)
                     /locus_tag="AC159P_16760"
                     /inference="COORDINATES: protein motif:HMM:NF024753.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HIR3697147.1"
                     /translation="MKRTLLLVTLLCLTAAARAQNWPQFRGPRAAGVADGKKTPVAWD
                     ASKPSNVAWKTPIPGLSHSSPVVWGNKIFLVTAISAEATPTYAAKNRGIGLARDEARH
                     AWKVYALDKATGKILWERTAHEGVPRAQRHVKATQANSTPATDGRTVVALLGSEGLFA
                     YDTSGKLLWKQDLGVLNPGLWSDPTSSWGHASSPVIHGQLVFVQADGHKQSFIAAFNL
                     KDGKQAWRVERGEITSWTTPTVYEGKTRTELIANGGRFIRGYDPATGKELWRFADGDT
                     QVKMQAPLVAHDLIFITGGYPAGRPMYAFRPGASGDISLKAGEETNAFLAWKTAKGSP
                     YTPTPVVYGDLLYAAADNGVFSAYDAKTGALLYQERLPSSFSASPVAADGKIYVPSED
                     GEVFVVRAGPKFELLATNPMGEPLMATPALSGGMLILRS"
     gene            complement(1501..3012)
                     /locus_tag="AC159P_16765"
     CDS             complement(1501..3012)
                     /locus_tag="AC159P_16765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HIR3697148.1"
                     /translation="MRNKRTLVTTMAAVLIAALCCGQLLTAHGSRLTASDPGSGDWPM
                     WGGTADRNMVSKAKGMPTSWDVKTKKNIKWVSELGSQTYGNIVVSGGVVLVGTNNEAK
                     RDPKITGDKGVLMAFDEKTGAFLWQMVHDKLVAGRVNDWPFQGIASSPLVEGDRVYYV
                     SNRAELMCVDLQGFRDKENDGPVTDEKAKEETHGDVVWRFDMMEEVGTLPHNLANSSP
                     VTWGDLIYVSTSNGQDESHVNVPAPKAPAIVAVNKKTGKLVWEDASPGEKILHGQWST
                     PTVGKLGGVDQVIMGQGDGWVRSYEAATGKKLWEFDTNPKDSVWPKTRNEIISTPVLY
                     EDKVYLANGQDPEHGEGVGHMYCIDPTKRGDITKTGLVWHYDKIRRSISTPAIVDGII
                     YQPDFSGFLHAIDAKTGAQIWVHDMFAAVWGSPLVTADGRLYLGDEDGDVVIMQTGKV
                     KKVLAEINMGSSVYSTPVPANGALFIANRNQLFALAEGATTTTAVPAKPAASQ"
     gene            complement(3091..4191)
                     /locus_tag="AC159P_16770"
     CDS             complement(3091..4191)
                     /locus_tag="AC159P_16770"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020706843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3697149.1"
                     /translation="MTTDDNVTTAANAHDDATAETNAPPAPETHAGQQGPNPERLFQM
                     LTAYQASAALKAAIDLDIFTAVAEGADTAAALAESRGVAERGARILCDYMTVLQLLSK
                     EDGRYRLTPESEAFLDRRSPAFLGGMAGFLLSPPLLDAFRDLTESVRKGGTTMHAQGS
                     MTPEHPMWEEFARSMTAMAGPASEAIASIVGADAMETCKVLDIAASHGVYGITLARRN
                     PRAQVYALDWQNVLPFARANAEAAGVAERFHEIPGDAFEVDFGTDYDLVLLPNFFHHF
                     DQPTCVRLMRKVHAALKDGGRAVTLEFVPDETRVAPPASATFPLVMLSSTPAGDAYTF
                     SEYDEMFREAGFSRSESHPAAAAPQHIIVSYK"
     gene            complement(4206..>5231)
                     /locus_tag="AC159P_16775"
     CDS             complement(4206..>5231)
                     /locus_tag="AC159P_16775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697150.1"
                     /translation="DQLGTEALPESIIASVAVSHGRVYLVTDTALYAIGKKAARGTSS
                     SPASIRNAGHAVEGAQGAASHVQVVPAEVTLKPGETVSFRVRLFDERGNFIREEKGAT
                     WALDKLDGKAEAGKFTAASDAVAQAGEVKATVGGVTGAARVRVIPGLPWDENFDKMEA
                     GTFPQHWTNTGMKYAVREVEGNKVLVKTIEGSSLLSRARAYAGPVEWSNYTIEVDTKA
                     FEKRRQLGDAGVLAQRYALVLFGNAQKLELSPWQPETARVAAVPFAWKQDAWYRLKLQ
                     VENLPNGQVRARGKAWPVGEPEPAAWLVERTDPIGNRQGSPGLFGAALTAEIYFDNLK
                     VYPNKQS"
BASE COUNT          731 a         1754 c         1759 g          987 t
ORIGIN      
        1 tgcgaccgca ggatcagcat cccgcccgag agggcgggcg tggccatcag cggctcgccc
       61 atcgggttcg tggccagcag ctcgaacttc ggcccggccc tgacgacgaa gacctcgccg
      121 tcctcgctcg gcacgtaaat cttcccgtcc gccgcgaccg gcgaggcgct gaacgaactc
      181 ggcagccgtt cctgataaag gagcgcgccg gtcttcgcgt cataggccga gaagacgccg
      241 ttgtcggcgg cggcgtagag caggtcgccg tagacgaccg gcgtcggcgt gtaggggctg
      301 cccttcgccg tcttccacgc gaggaacgcg ttggtctctt cgcccgcctt cagggagatg
      361 tcgccgctcg cgcccggtcg gaaggcgtac atcggtcggc ccgccgggta gccgcccgtg
      421 atgaagatca gatcgtgcgc gacgagcggc gcctgcatct tgacctgcgt gtcgccgtcg
      481 gcgaagcgcc acagctcttt gcccgtcgcg gggtcgtagc cgcgtatgaa gcgaccgccg
      541 ttggcgatca gctcggtgcg cgtcttgcct tcgtagacgg tgggcgtcgt ccacgacgtg
      601 atctcgccgc gctcgacgcg ccacgcctgc ttgccgtctt tgagattgaa ggcggcgatg
      661 aaggactgct tgtggccgtc ggcctggacg aagacgagct ggccgtggat gacgggcgag
      721 gaggcgtggc cccacgaaga ggtcgggtcg ctccacagtc cggggttgag cacgccgagg
      781 tcttgcttcc agagcagctt gcccgacgtg tcgtaggcga agagaccttc cgagccgagg
      841 agcgcgacga cggtgcgccc gtcggtcgcg ggcgtcgagt tggcctgcgt cgccttgacg
      901 tggcgctgcg cgcgcggcac gccttcgtgc gccgtgcgct cccacagaat cttgcccgtc
      961 gccttgtcga gcgcgtagac cttccacgcg tggcgcgcct cgtcgcgcgc cagaccgatg
     1021 ccgcggttct tggccgcgta ggtcggcgtc gcttcggcgc tgatggccgt gacgaggaat
     1081 atcttattgc cccacacgac cgggctggag tgcgagagtc cggggatcgg cgtcttccac
     1141 gcgacgttcg agggcttcga cgcgtcccac gcgaccggcg tcttcttccc gtcggcgacg
     1201 cccgcggcgc gcggcccgcg gaactgcggc cagttctgcg cgcgcgccgc agccgtcagg
     1261 caaagaaggg tgacgaggag gagagtgcgt ttcatgttcg tcgttcttcg gtctgcgcga
     1321 cgctcgagcg tcggcgcgag ctagttttca acgcggaggg gcgcggagca cgcagagaaa
     1381 acaaatttca gatttgaaat ttcaaaaccc gaatttcaaa tcatgtgttc ttctctgcgc
     1441 tctctgcgtt tctctgcgtc ctccgcgtta aagttaaagt cccgcgacac acgaccgcgc
     1501 tcactgcgag gcggcgggct tcgcggggac ggcggtcgtg gtggtcgcgc cctcggcgag
     1561 cgcgaagagc tggttgcggt tggcgatgaa gagcgcgccg ttggcgggga cgggcgtgct
     1621 gtagacggaa ctgcccatgt tgatttccgc cagcaccttc ttcaccttgc cggtctgcat
     1681 gatgaccacg tcgccgtcct cgtcgccgag gtagagcctg ccgtcggcgg tcaccagcgg
     1741 cgaaccccac acggcggcga acatgtcgtg cacccaaatc tgcgcgcccg tcttcgcgtc
     1801 tatcgcgtgc agaaagccgc tgaagtccgg ctggtagatg atgccgtcca cgatggcggg
     1861 cgtcgagatc gagcggcgga ttttgtcgta gtgccagacc agccccgtct tcgtgatgtc
     1921 gccgcgcttg gtcgggtcga tgcagtacat gtggccgacg ccctcgccgt gctcggggtc
     1981 ttggccgttg gcgagataaa ccttgtcttc gtagaggacg ggcgtcgaga tgatctcgtt
     2041 gcgcgtcttc ggccagacgg agtccttcgg gttggtatcg aactcccaca gcttcttgcc
     2101 cgtggcggcc tcgtaactgc gcacccagcc gtcgccctgc cccatgatga cctgatcgac
     2161 gccgccgagc ttgccgaccg tgggggttga ccactgcccg tgcagaatct tctcgccggg
     2221 gctggcgtcc tcccagacga gcttgcccgt cttcttgttg acggcgacga tggccggcgc
     2281 cttcggcgcg gggacgttga cgtggctctc gtcctgcccg ttcgaggtgg agacgtagat
     2341 cagatcgccc cacgtgacgg gcgaggagtt ggcgaggttg tgcgggagcg tgccgacctc
     2401 ctccatcatg tcgaagcgcc acacgacatc gccgtgcgtc tcttccttcg ccttctcgtc
     2461 ggtgacgggg ccgtcgttct ccttgtcgcg gaagccttgc aggtcaacgc acatcagctc
     2521 cgcccggttc gagacgtagt agacgcggtc gccctcgacc agcggcgaag aggcgatgcc
     2581 ctggaacggc cagtcgttga cgcggcccgc gaccagcttg tcgtgcacca tctgccagag
     2641 gaaggcgccg gtcttctcgt cgaaggccat cagcacgccc ttgtcgcccg tgatcttcgg
     2701 gtcgcgcttc gcctcgttgt tcgtcccgac cagcacgacg ccgcccgaga cgacgatgtt
     2761 gccgtaggtc tgcgacccca gctccgacac ccacttgatg ttcttcttcg tcttcacgtc
     2821 ccaagacgtg ggcatcccct tcgccttcga caccatgttg cgatccgccg tcccgcccca
     2881 catcggccag tcgccgctgc cggggtcgga agccgtgagc cgtgagccgt gagccgtgag
     2941 cagttgccca caacacagcg ccgcgattaa caccgccgcc atcgtcgtca ccaacgttct
     3001 tttatttctc atcatctttt tctttcattg ccccgctgac tgcttccgcc ttcaatcctc
     3061 atcgcccact cttcatttac tgctcaccgc tcacttgtaa gagacgatga tgtgttgcgg
     3121 cgccgccgcc gcggggtggc tctcgctgcg cgagaagccc gcctcgcgga acatctcgtc
     3181 gtactccgag aaggtgtagg cgtcgcccgc gggcgtgctg ctgagcatga cgagcgggaa
     3241 ggtggccgac gcgggcggcg ccacgcgggt ctcgtcgggc acgaattcga gcgtcacggc
     3301 gcggcctccg tctttcagcg ccgcgtggac tttgcgcatc agccggacgc aggtcggctg
     3361 gtcgaagtgg tggaagaagt tgggcagcag cacgaggtcg tagtcggtgc cgaagtccac
     3421 ctcgaaggcg tcgcccggta tctcgtggaa gcgctcggcg acgcccgccg cttcggcgtt
     3481 ggcgcgcgcg aacgggagca cgttctgcca atcgagggcg taaacttgcg cgcgcgggtt
     3541 gcggcgcgcg agcgtgatgc cgtagacgcc gtggctcgcg gcgatgtcga gcaccttgca
     3601 ggtctccatc gcgtcggcgc cgacgatgga ggcgatggcc tcggacgccg gccccgccat
     3661 cgcggtcatg ctgcgcgcga actcctccca catcgggtgc tcgggcgtca tcgagccttg
     3721 cgcgtgcatg gtcgtgccgc ccttgcggac gctctcggtc aggtcgcgga aggcgtcgag
     3781 caggggcggg ctgagcagaa atcccgccat gccgccgagg aacgcgggcg agcggcggtc
     3841 gagaaatgcc tccgactcgg gcgtcaggcg gtagcgcccg tcctccttgc tcaggagctg
     3901 caagacggtc atgtagtcgc agaggatgcg cgcgccgcgc tcggcgacgc cgcgactctc
     3961 cgcgagcgcg gcggcggtgt ccgcgccttc ggcgacggct gtaaagatgt cgaggtcgat
     4021 ggcggccttc agcgccgcgc tcgcctgata ggcggtcagc atttgaaaca ggcgctcagg
     4081 gttcggcccc tgctgcccgg cgtgcgtctc cggcgcgggc ggcgcgttcg tctccgcggt
     4141 cgcgtcgtcg tgtgcgttcg ctgctgtggt cacgttgtcg tctgtcgtca ctttgtcttt
     4201 gtccgtcagc tttgtttgtt ggggtagacc ttcaggttgt cgaaatagat ttccgccgtc
     4261 agcgcggcgc cgaagaggcc ggggctgccc tgccggttgc cgatggggtc ggtgcgttcg
     4321 accagccacg cggcgggttc cggctcgccc acgggccacg ccttgccgcg cgcgcgcacc
     4381 tgcccgttcg gcaggttctc gacctgcaac ttcaggcgat accaggcgtc ctgcttccac
     4441 gcgaagggga cggcggcgac gcgcgccgtc tcgggctgcc acggcgagag ttcgagcttc
     4501 tgcgcgttgc cgaacagcac gagcgcgtag cgctgcgcga gcacgcccgc gtcgccgagc
     4561 tgccgccgct tctcgaacgc cttcgtgtct acctcgatgg tgtagttcga ccactcgacc
     4621 ggccccgcgt aggcgcgcgc gcggctcagc agcgaagagc cttcgatggt cttcaccaag
     4681 accttgttgc cttccacctc gcgcaccgcg tacttcatcc ccgtgttcgt ccagtgctgc
     4741 gggaaggtgc ccgcctccat cttgtcgaag ttctcgtccc acggcaaacc ggggatgacg
     4801 cgcacgcgcg ccgcgcccgt cacgccgccg accgtcgctt taacctcgcc ggcctgcgcg
     4861 accgcatcgc ttgcggcggt gaacttaccc gcctcggcct tgccgtcgag tttgtcgagc
     4921 gcccacgtcg cgcccttttc ttcgcggatg aagttgccgc gctcgtcgaa caggcgcacg
     4981 cggaagctca ccgtctcgcc cggcttcaga gtcacttcgg cgggcacgac ctgcacgtgc
     5041 gacgccgcgc cctgtgcgcc ttcgacggcg tgcccggcgt tccggatgga cgccggcgaa
     5101 gacgacgtgc cgcgcgcggc cttcttcccg atggcgtaga gcgccgtgtc ggtgacgagg
     5161 tagacgcgcc cgtgcgagac ggcgaccgag gcgatgatcg attcgggcag cgcctctgtg
     5221 ccgagctggt c
//
LOCUS       DBNGOH010000867         1875 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3430054, whole genome shotgun sequence.
ACCESSION   DBNGOH010000867 DBNGOH010000000
VERSION     DBNGOH010000867.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1875)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1875)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1875
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3430054"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(23..481)
                     /gene="rplM"
                     /locus_tag="AC159P_16780"
     CDS             complement(23..481)
                     /gene="rplM"
                     /locus_tag="AC159P_16780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006555776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HIR3697151.1"
                     /translation="MSTYFPSGDTLAENRKWYVVDAAGQTVGRLATEIARVLSGKNNP
                     RYTPFMDTGDHVIVINAKKAVFTGAKAQQKVYRHHTLYPGGLKEVSATEQFAKHPARV
                     IESAVKGMLPKNKLGKAMAKKLKVYGGAEHPHAAQQPEPLPFAERGGIKK"
     gene            complement(726..>1875)
                     /locus_tag="AC159P_16785"
     CDS             complement(726..>1875)
                     /locus_tag="AC159P_16785"
                     /inference="COORDINATES: protein motif:HMM:NF013594.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HIR3697152.1"
                     /translation="AVVLSLLTGSLTAAAAPRRAQQQQDAQKTAQKEASGKKDDKKKE
                     QQTTPATGKSLGSKEDPRLVGKRNINTGIIGGGSVEKEVRLGQQLAAEVDRQAKFVDD
                     PMITEYVNRVGQNIVIHSDAKIPFTIKVIDSDEANAFALPGGFFYVNKGLLLAADNEA
                     ELAGVMAHEIAHVTARHAMENMRKAQIAEIGATIGSIFLGGIPGMIYQSTAGLGLTAL
                     FLKFSRGAEEEADRLAAQYLWAAGYDPGAMSTMFEKLNTKNKKKPGFISRAFSTHPQT
                     PDRLEASRALVARFPEREEYVLSTSEFQRVKARMLRLSNARASTVGDITDEEGNRRPT
                     LKRRAPAPDDPAATDGATEEKKTEEAPKLKRRTDDQTAPAEQQKGDKP"
BASE COUNT          243 a          574 c          672 g          386 t
ORIGIN      
        1 ttctcgtcct ttggtaaatc ggttacttct tgatgccgcc gcgttcggcg aacgggagcg
       61 gctcgggctg ctgcgccgcg tgcgggtgct cggcgccgcc gtagaccttg agcttcttcg
      121 ccatcgcctt gccgagcttg ttcttcggga gcatgccctt gaccgccgac tcgatgacgc
      181 gcgcggggtg cttggcgaac tgctcggtgg cggagacctc tttgaggccg ccggggtaga
      241 gcgtgtggtg gcggtagacc ttctgctggg ccttcgcgcc ggtgaagacg gccttcttgg
      301 cgttgatgac gatgacgtga tcgcccgtgt ccatgaaggg tgtgtagcgc gggttgttct
      361 tgccgctgag cacgcgcgcg atttcggtcg cgaggcgccc gacggtctgc ccggcggcgt
      421 ccaccacgta ccacttgcga ttctcggcga gcgtgtcgcc gctgggaaag tatgttgaca
      481 tagataactc tctcaattta aagcgtctaa aacgcgaacg ggtagaatac gaaacgaccg
      541 cgaaacttgt caagctgcgg aggatgcggc gtgcgtgact ttcgcttgtg ccggggggcc
      601 tatcggggcg gggcgctgcc taaatcccac ggtagcttag cctgaaacca ctgactccca
      661 caagccgaag gctctacggg ccgcggcccg tagagccttc gctgtcggtc gaaccccgcg
      721 aggggttacg gcttgtcgcc cttctgctgc tcggcgggcg cggtctggtc gtccgtgcgg
      781 cgcttgagct tgggcgcttc ctcggtcttc ttctcttcgg tcgcgccgtc ggtcgccgcc
      841 gggtcgtcgg gcgcgggcgc gcggcgcttc aaggtcgggc ggcggttgcc ctcttcgtcc
      901 gtgatatcgc cgacggtcga ggcgcgcgcg ttcgacagac gcagcatgcg cgccttgacg
      961 cgctggaact cggaggtcga gagcacgtac tcctcgcgct cggggaagcg cgcgacgagg
     1021 gcgcgcgagg cttcgaggcg gtcgggcgtc tgcgggtgcg tcgagaaggc gcgcgagatg
     1081 aagccgggct tcttcttgtt cttcgtgttc agcttctcga acatggtgga catcgcgccg
     1141 gggtcgtagc cggcggccca gaggtactgc gcggcgaggc ggtcggcctc ctcttcggcg
     1201 ccgcggctga acttcaggaa gagcgccgtc aggccgaggc cggcggtgct ctggtagatc
     1261 atgccgggga tgccgccgag gaagatcgag ccgatggtgg cgccgatctc ggcgatctgc
     1321 gccttgcgca tgttctccat cgcgtggcgc gcggtgacgt gcgcgatctc gtgcgccatc
     1381 acgccggcca actcggcctc gttgtcggcg gccaagagca ggcccttgtt gacgtagaag
     1441 aacccgcccg gcagcgcgaa ggcgttggcc tcgtccgagt cgatgacctt gatggtgaag
     1501 ggaatcttcg cgtcggagtg gatgacgatg ttctggccga cgcggttgac gtactcggtg
     1561 atcatcgggt cgtcaacgaa cttggcctgg cggtcaacct ctgcggcgag ctgctggccg
     1621 aggcggactt ccttctcgac ggagccgccg ccgatgatgc ccgtgttgat gttgcgcttg
     1681 ccgacgagcc gcgggtcttc cttggagccg agcgacttgc cggtggcggg cgtcgtctgc
     1741 tgctccttct tcttgtcgtc cttcttgccg gacgcctctt tctgcgcggt cttctgcgcg
     1801 tcctgctgct gctgcgcgcg gcgcggcgcg gcggcggcgg tcagcgagcc ggtcagcaga
     1861 ctgaggacga cagcc
//
LOCUS       DBNGOH010000868         4014 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2697524, whole genome shotgun sequence.
ACCESSION   DBNGOH010000868 DBNGOH010000000
VERSION     DBNGOH010000868.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4014)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4014)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4014
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2697524"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1102)
                     /locus_tag="AC159P_16790"
     CDS             complement(<1..1102)
                     /locus_tag="AC159P_16790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP repeat domain-containing protein"
                     /protein_id="HIR3697153.1"
                     /translation="MNRKFLFLFCLVAAACLAAAPAAPSARAAAQSSAPVTFAEVSPR
                     SSGLAWVHENARSPERYLPETIGPGCVFFDFDNDGWMDIFLVNSGAADFYTPTRPLKH
                     ALYRNNRDGTFTDVTDKAGVRGGQHFGQGAAAGDYDGDGWQDLYVTAYGSAALYRNRG
                     DGTFADVTQKAGVAAPGWSTCATWFDYDNNGTLDLFVSNFVQYNKYLSCGDKRANRNY
                     YCIPRVFKPRPSQLFRNNGDGTFTDVSKESGVVGSLGKAFSSVATDINNDGLLDLFVA
                     NDTVANFLFLNKGGGKFEEIGLEAGVAYSESGVARSGMGVDAADFDGDGWQDLFVANI
                     DMELFSLYRNQKDLTFTDEPGEIAPATRLLSGW"
     gene            complement(1184..2314)
                     /locus_tag="AC159P_16795"
     CDS             complement(1184..2314)
                     /locus_tag="AC159P_16795"
                     /inference="COORDINATES: protein motif:HMM:NF045090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3697154.1"
                     /translation="MVLALAIAPAQGDSARRESEAHFARGVEFQQKGEFEGARAEYEA
                     ALKLVPRRPEALSNLGVVYARLGRYDEAIRRYAEALALDPQQYATRLNLGIAHFQTGH
                     FEEAQRELRKVVAAESGNDQARLLLGLCHLQLNELSEAVSELETVYRRQPGNVGAAYA
                     LGTAYIGLNRLERAQELIDAVFRRLDSAEAHLLMGSLSVAAKDFTRAIDELNQARQIN
                     ARLPTLHSMLGNAYLLSGGREEAVKAFQSELEINPRDFDANSRLGVLYREDGRLDEAE
                     RLLGKALALRPDDTYTLYQMAQLRQARGANEEAVRLLERVVERLPDFTQAHVLLARLY
                     FKLKRTAEAERERAIINRLNAEQQKKQLLSKPGEAQPAPNPR"
     gene            complement(2545..3594)
                     /locus_tag="AC159P_16800"
     CDS             complement(2545..3594)
                     /locus_tag="AC159P_16800"
                     /inference="COORDINATES: protein motif:HMM:NF025006.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3697155.1"
                     /translation="MRLRPTRLRRVARAACRLSLALTLSCICPSHARAQQSDAPDESR
                     RRYTIQGQVTLPDGRPAANARVKLTGTLGVNREALANDAGRYEFAELPAGSYQLEARS
                     PTGGNPAFGSVETHTTRTATNSLTANIAMRAEAVKTSEPKPAVVSAADAGQKVPPQAR
                     KAFEEGVKFRGSNDPAKALASFTQAIELYPAYYQALAARADIHLSRRRLSEAAADFDH
                     ALKSNPRYGAALRGAGYCKLERGQFAEAAEYFDKAVVDEPESARSFLLLGMANFELDR
                     REQAKLALQQALKIDAAQSVRARLYLANIHAREGQFRRAADELRTYLEAAPSDPEAAE
                     LRKVEALWRARPDGQ"
     gene            complement(3806..>4014)
                     /locus_tag="AC159P_16805"
     CDS             complement(3806..>4014)
                     /locus_tag="AC159P_16805"
                     /inference="COORDINATES: protein motif:HMM:NF024834.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR3697156.1"
                     /translation="GRGQTALGMKLKAPNFTDSRWQKDISDSRMRESITEGSDDMPPF
                     GRKLSTQEIASLVAHIRGFAEAER"
BASE COUNT          592 a         1272 c         1415 g          735 t
ORIGIN      
        1 cccagccgga gagcaaccgc gtggcgggcg cgatctcgcc cggctcgtcc gtgaaggtta
       61 aatccttctg gttgcggtag aggctgaaca gctccatgtc gatgttggcg acgaagaggt
      121 cttgccagcc gtcgccgtcg aagtcggcgg cgtccacgcc catgccggag cgcgcgacgc
      181 cggactcgct gtaggcgacg cccgcctcaa gcccgatctc ctcgaacttg cccccgccct
      241 tgttgaggaa gaggaaattg gcgaccgtgt cgttggcgac gaagagatcg agcaggccgt
      301 cgttgttgat gtcggtcgcc accgagctga aggctttgcc gagcgagccg acgacgccgg
      361 actccttgct cacgtccgtg aaagtcccgt cgccgttgtt gcggaagagc tggctcggcc
      421 tcggcttgaa gacgcgcggg atgcagtagt agttgcggtt cgcgcgcttg tcgccgcagc
      481 tcaggtactt gttgtactgg acgaagttcg agacgaagag gtcgagcgtg ccgttgttgt
      541 cgtagtcgaa ccacgtcgcg caggtggacc agccgggcgc ggcgacgccc gccttctgcg
      601 tcacgtcggc gaaggtgccg tcgccgcggt tgcggtagag ggcggcgctg ccgtaggccg
      661 tcacgtacaa gtcctgccag ccgtcgccgt cgtagtcgcc cgcggcggcc ccctgcccga
      721 agtgctggcc tccccggacg cccgccttgt cggtcacgtc cgtgaaagtg ccgtcgcggt
      781 tgttgcggta gagggcgtgc ttgagcggcc tggtcggcgt gtagaagtcc gccgctccgc
      841 tgttgacgag gaagatgtcc atccagccgt cgttgtcgaa gtcgaagaag acgcagcccg
      901 gcccgatggt ctcgggcagg tagcgctcgg gcgagcgcgc gttctcgtgc acccaggcga
      961 ggccgctcga cctgggcgac acctccgcga aggtgacggg cgccgacgac tgcgccgcgg
     1021 cgcgcgcgga cggggctgcg ggcgccgccg ccaggcaggc cgccgccacg agacagaaga
     1081 gaaagagaaa cttacgattc aagacgctaa ccttccagac tcctagccgg gtcgaatcgg
     1141 cggcgtgtgc gccccgcacg gcgacaacgg cttccgtaac tgctcagcgg ggattcggcg
     1201 cgggctgcgc ctcgcccggc tttgagagca actgtttctt ctgctgctcg gcgttgagcc
     1261 ggttgatgat ggcgcgctcg cgctcggcct ccgctgtgcg tttcagcttg aagtagaggc
     1321 gggcgagcag gacatgcgcc tgcgtgaagt ccggcagccg ctcgaccacg cgctccagca
     1381 gcctgaccgc ctcctcgttc gcgcccctcg cctgccgcag ttgcgccatc tggtagagcg
     1441 tgtaagtgtc gtcgggccgc agcgcgagcg ctttgccgag cagccgctcg gcttcgtcca
     1501 agcgcccgtc ctccctgtag aggacgccca accgggagtt cgcgtcgaag tcgcgcgggt
     1561 tgatttccaa ctcggactgg aacgccttga cggcctcttc gcgcccgccc gaaaggaggt
     1621 aggcgttgcc gagcatggag tggagcgtgg ggaggcgggc gttgatttgt cgggcctggt
     1681 tcaactcatc gatggccctc gtgaaatctt tggcggcgac gctcagcgat cccatcagca
     1741 ggtgcgcttc cgcggaatcg agacggcgga agaccgcgtc gatcaactcc tgcgcccgct
     1801 ccaaccggtt gaggccgatg taggccgtgc cgagggcgta ggcggcgccg acgttccccg
     1861 gctgcctgcg gtagacggtt tcgagttcgg agacggcctc gctcagttcg ttcagttgta
     1921 agtggcacag cccgagcagc aggcgcgcct ggtcgttgcc cgactcggcg gcgaccacct
     1981 tccgaagttc gcgttgcgcc tcttcgaagt gtcccgtctg gaaatgtgcg atccctaagt
     2041 tgagccgtgt ggcgtactgc tgcgggtcga gcgcgagggc ttcggcgtag cgcctgatcg
     2101 cctcgtcgta ccggcccagg cgcgcgtaga cgacgccgag gttcgagagg gcttcggggc
     2161 gacggggcac gagtttgagc gccgcttcat attcggcgcg cgcgccctcg aactcgccct
     2221 tctgctgaaa ctcgacgccg cgggcgaagt gggcctccga ctcgcgccgc gccgagtcgc
     2281 cctgtgcggg ggcaatcgct aaggcgagaa ccaagactaa gccgggtgca atcatcagaa
     2341 cttcgattga tgttgcggag tgtgaatgaa cccggtcgcg cgcaagataa tggagcgtgc
     2401 cggatcgttc ggtgagctcg aagtatagca cccgccgacg gcatcggcaa acatgcggtt
     2461 cgccggtaag tggcggaact tactcgcttt gcggggtgcg ccggcttcag ccgacgagtg
     2521 agcaaaagtc ggcggatgaa atactcactg accgtcgggg cgggcgcgcc acagggcttc
     2581 caccttcctc aactccgcgg cctcggggtc tgacggcgcc gcctccaggt aagtgcggag
     2641 ttcgtccgcg gcccgccgga attgcccctc gcgcgcgtgt atgttggcga ggtagaggcg
     2701 cgcgcggacg gactgcgcgg cgtcgatctt gagcgcctgt tggagggcga gcttggcctg
     2761 ctcccgccgg tcgagttcga agttggccat cccgagcagg aggaaacttc tcgcgctctc
     2821 cggttcgtcg acgacggcct tgtcgaaata ctcggcggcc tctgcgaact gcccccgctc
     2881 cagcttgcag tacccggcgc cgcgcagcgc ggcgccgtag cgcgggttgc tcttcagggc
     2941 gtggtcgaag tccgcggcgg cctcggacag tcggcgtctg ctcaggtgta tgtcggcgcg
     3001 cgcggcgagg gcttggtagt aagcggggta gagttcgatt gcctgagtga aactggcgag
     3061 agctttggcc ggatcgttac tgcccctaaa cttgacccct tcctcgaaag ccttgcgagc
     3121 ttgcggcggc accttctgcc ccgcatctgc cgcggagacg accgccggtt taggctcgga
     3181 ggtcttcacc gcctccgcgc gcatcgctat gttggcggtc agtgaattcg tggcggtgcg
     3241 ggtcgtgtgc gtctcgaccg agccgaaggc gggattgccg cccgtcgggc tgcgggcctc
     3301 cagttggtag ctgcccgccg ggagttcggc gaactcatac ctgccggcgt cgttcgccag
     3361 cgcctcgcgg ttgacgccga gtgtgcccgt gagtttgacg cgggcgttcg ccgcgggcct
     3421 gccgtcgggc agtgtgacct ggccctgtat ggtgtagcgt cgtctgctct cgtccggcgc
     3481 gtcgctctgt tgcgcgcgtg cgtgcgacgg gcagatgcaa ctcaacgtga gtgcgagcga
     3541 gaggcggcag gcggcgcgtg cgacgcggcg gagccgagtt ggacggagcc tcatcaacct
     3601 ccctcctcgt tgaaggacaa ggagcggtag cgaccgccgc ggggatgggc ttgcgtcgcg
     3661 agggccgcgc tcgcggcggg cgggtgacgg cacgggaaac gcagggatta taacggctcg
     3721 ttcaaccccg cgccaacgcc gaggcgaggc gcggggtcgt gtgacggtct cctgtgcatt
     3781 cgggcggagg cgccagtcca tcccttcagc gctcggcctc tgcgaagccg cggatgtgcg
     3841 cgacgagcga cgcgatctct tgtgtggaga gcttcctgcc gaagggcggc atatcgtcgc
     3901 tgccctccgt gatggactcg cgcatgcgcg agtcgctgat gtctttctgc catctactgt
     3961 ctgtaaaatt cggagccttc agcttcatcc ccagcgcggt ctgtccgcgc ccgt
//
LOCUS       DBNGOH010000869         3773 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2146540, whole genome shotgun sequence.
ACCESSION   DBNGOH010000869 DBNGOH010000000
VERSION     DBNGOH010000869.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3773)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3773)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3773
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2146540"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1227)
                     /locus_tag="AC159P_16810"
     CDS             complement(<1..1227)
                     /locus_tag="AC159P_16810"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013882.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1901271 - lipooligosaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 25 protein"
                     /protein_id="HIR3697157.1"
                     /translation="MNEADRERRTRSIDEAFPHRVCINLDRRPERWRAMRAKFARHGI
                     HAVRRFAALDGRALDIPPHWVHTPGAYGCLRSHVEVVREARRLGAKSVLVFEDDVVFD
                     PELREKFAARVGELPRDWDMLYFGALHKDEPVRVTDNIFRLTRSNSTYAYALRETVFD
                     AFVELNDRAEDVLDNNGFLLQRRFNCYCFMPHLAWVETDYSDAQQRLERHWYLKESLV
                     LFGPRADALLADTTLVLAHHARVGAKGAAENLTFLVDYYDFFFSPHLAVVVVEQGARP
                     SLDPATLPPRCAYVFLRDEGGFDRARCFAAGVEKSGPGRNIFILSDSDIYLETLDIRA
                     NLRMCERHDGATGFDHVFDLSDEDSRLLRRTKITRGIDLTKSTPAGGARAGRCRFLRR
                     EAIGKAETHLRLFHSPN"
     gene            1424..2236
                     /locus_tag="AC159P_16815"
     CDS             1424..2236
                     /locus_tag="AC159P_16815"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017532.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HIR3697158.1"
                     /translation="MTEGVRSILTETQLAEYEELGFVHSIPVLSEAEVRRYRAEVERT
                     CAALGGGVTRLDGLHLFFRWAWDLCTHPRLLDCMEQLLGPDILLKSTRLFYKHGHSAS
                     FVGWHQDGITERLADGRVPAVWLGLTEATIENGCLRVAPRSHRLGLIPHAPRPHADNL
                     TTEGVTALAEVAEAYDIVMRPGEMSLHHPLILHASNPNRSDTSRIGFTATYSTAALTT
                     SRTAVAWVRGDGPRDCFEIADRPPACSLAEAVAAYRARDHQKRFAGQDSDAY"
     gene            2504..3358
                     /locus_tag="AC159P_16820"
     CDS             2504..3358
                     /locus_tag="AC159P_16820"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR3697159.1"
                     /translation="MSSPPLLSVVIPTWNRARLVCEAVESALAQRPGGVEVIVVDDGS
                     TDGTAEVLACRFGPRVRVLRQASRRGPGAARNAGVREARGHLLAFLDSDDLWLPGKLD
                     AELGVLERFPDAEVVVSDSLNFLEGRADEQSRFEQNGLLAATGGEPRWMSQCRWLWTN
                     IQNNMATCSITLRRTALAHFAGPLFAEDLTCCEDWEFEMRLHRYCRVVVLPRVLASVR
                     RFDDGARPGRAVPGKPFTREQKLGLLRDRLTVMERAHWLDGLDADLVGELLRFRDETS
                     RQLKESEQ"
     gene            3472..>3773
                     /locus_tag="AC159P_16825"
     CDS             3472..>3773
                     /locus_tag="AC159P_16825"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3697160.1"
                     /translation="MEEVHLWRGSPEQRALRAQAGRSGQFAYFDRQLGHPAWGGMKVL
                     DFGGNTGNLLASAADAIRHEDYYCLDIIRDALEVGRERFPRAHFVHYDRYNCSFNP"
BASE COUNT          608 a         1288 c         1273 g          604 t
ORIGIN      
        1 gttcggggag tggaaaagcc gcaggtgagt ttccgctttc cctatcgcct cgcgtctcaa
       61 gaagcgacag cggcccgcgc gcgcgccgcc cgcaggagtg cttttcgtga ggtcgatgcc
      121 gcgcgtgatc ttcgtgcggc gcagcagccg agagtcctcg tcgctcaggt cgaagacgtg
      181 gtcgaagccc gtggcgccgt cgtggcgttc gcacatccgc aggttggcgc ggatgtcgag
      241 ggtctctaaa tagatgtcgc tgtcggagag gatgaagatg ttccggcccg gcccggactt
      301 ttcgaccccc gccgcgaaac agcgcgcgcg gtcgaagccg ccttcgtcgc gcaggaaaac
      361 gtaggcgcac cgcggcggca gcgtcgcggg gtcgagggaa ggccgcgcgc cctgctcgac
      421 gacgacgacg gcgaggtgcg gcgagaagaa gaagtcgtag taatcgacga ggaacgtcag
      481 gttctccgcc gcgcccttcg cgcctacgcg cgcgtggtgc gcgaggacga gcgtcgtgtc
      541 cgcgagcaga gcgtccgccc gcgggccgaa gaggacgagc gattccttca gataccagtg
      601 tcgctcaagg cgctgctgcg cgtccgagta gtcggtctcg acccacgcga ggtgcggcat
      661 gaagcagtag cagttgaagc gccgctgcaa caggaagccg ttgttgtcca gcacgtcctc
      721 ggccctgtcg ttcagctcca cgaaggcgtc gaacaccgtc tcgcgcaggg cgtaggcgta
      781 ggtcgagttg gaacgcgtga ggcggaagat gttgtcggtg acccgcaccg gctcgtcctt
      841 gtggagcgcg ccgaagtaga gcatgtccca gtcgcgcggc agctccccga cgcgggccgc
      901 gaacttttcc cgcagctcgg ggtcgaagac cacgtcgtct tcgaagacga gcacgctctt
      961 cgcgccgagc cgccgggcct cgcggacgac ctcgacgtgg ctgcgcaggc acccgtaggc
     1021 gcccggcgtg tgaacccagt gcggggggat gtccaaagct ctgccgtcga gcgcggcgaa
     1081 gcggcggacg gcgtggatgc cgtgccgcgc gaacttcgca cgcatcgcgc gccagcgctc
     1141 cggcctgcgg tcgaggttga tgcacacgcg atgcgggaac gcctcgtcga tgcttcgcgt
     1201 gcgccgttct ctgtcggctt cgttcatggt tagctgttag ccattaggct gcgccacgga
     1261 ttttataggt gatacccgat agcgccaacg ccgaagacgc aggaaagctg acagccaacg
     1321 gctaacagct aacagccaac agctaatggc taaaattcgt tgacggctct ttgaccgtct
     1381 catacactgt ccgcgctcgg cgcgggaggc cgaaagaaga atgatgaccg aaggcgtgcg
     1441 ttccatcctt acagagacgc agttggcgga gtacgaggag ctggggttcg tccactcgat
     1501 ccccgtcctg tccgaagcgg aggtgcgccg ctaccgcgcc gaggtcgagc ggacgtgcgc
     1561 ggcgctcggc ggcggcgtga cgcggctcga cgggctgcac ctcttcttcc gctgggcctg
     1621 ggacttgtgc acgcacccgc gcctgctcga ctgcatggag caactgctcg gccccgacat
     1681 cctgctcaaa tcgacgcgcc tcttctacaa gcacgggcac agcgcctcct tcgtcgggtg
     1741 gcatcaggac ggcatcacgg agcgtctggc ggacgggcgc gtgcccgccg tctggctcgg
     1801 gctgaccgag gccacaatcg agaacggctg cctgcgcgtg gcgccgcgct cgcaccgcct
     1861 cggcctcatc ccgcacgcgc cgcgcccgca cgcagacaac ctgaccacgg agggcgtgac
     1921 cgcgctcgcg gaggtcgccg aagcctacga catcgtcatg cgccccggcg agatgagtct
     1981 gcaccacccg ctcatcctcc acgcctcgaa cccgaaccgc tcggacactt cgcgcatcgg
     2041 cttcacggcg acctactcga cggcggcgct gacgacgagc cgcaccgccg tcgcgtgggt
     2101 gcgcggcgac gggccgcgcg attgcttcga gatcgccgac aggccgcccg cgtgttcgct
     2161 cgcagaggcg gtcgccgcct accgcgcgcg cgaccaccag aagcgcttcg ccggccagga
     2221 ctccgacgcg tattaagtcg gtcgtaaaaa aagttcgtgc gtattgcggc tcggccattt
     2281 gcggttccgc cgcctgacgt gcggtgaggc tctgcctcac cggtgatgcg tcccttcgtg
     2341 tgctgaggct acgcctcagc acaggggcgt agcccctgaa cttacatggc actcatgccg
     2401 gaagggcaca gcccttccgc acatcaggcg gcggagccgc cggagtactc actcccattt
     2461 ttcaagagcc gccggaacta cggcgccaat tttcgaggac tcgatgtcgt cgcccccgct
     2521 cctatcggtc gtgatcccga cgtggaatcg tgcgcgcctc gtgtgcgagg cggtcgagag
     2581 cgcgctcgcg caaaggccgg gcggcgtcga ggtcatcgtc gtggacgacg gctcgaccga
     2641 cgggacggcg gaagtgctcg cgtgtcgctt cgggccgcgc gtgcgcgtgc tgcggcaggc
     2701 gagccgccgc ggccccggcg cggcgcgcaa cgccggggtg cgcgaggcgc gggggcacct
     2761 gctcgccttc ctcgactcgg acgacctctg gctgcccggc aagctcgacg ccgaactcgg
     2821 cgtgctcgaa cgcttccccg acgccgaggt ggtcgtctcc gacagcctga actttctgga
     2881 ggggcgggcg gacgagcaga gccgcttcga gcagaacggc ctgctcgcgg ccaccggcgg
     2941 cgagccgcgc tggatgagcc agtgccgctg gctgtggacc aacatccaga acaacatggc
     3001 gacgtgctcg atcacgctgc ggcgcacggc gctcgcgcac ttcgcggggc cgctcttcgc
     3061 cgaagacctc acgtgctgcg aggactggga gttcgagatg cgcctgcacc gctactgccg
     3121 cgtcgtcgtc ctgccgcgcg tgctcgcctc ggtgcgccgc ttcgacgacg gcgcgaggcc
     3181 gggccgcgcc gtccccggca agcccttcac gcgcgaacag aagctgggcc tgctccgcga
     3241 ccgcctcacc gtcatggagc gcgcccactg gctcgacggt ctcgacgccg acctcgtcgg
     3301 cgagctgctc cgcttccgcg acgagacctc gcgccaactc aaggagagtg agcagtgagc
     3361 ggtgagcagt gagcagtaac agcaaagaca ttcggcttaa cgctcacgcg ctttcttctt
     3421 ctcactgccc actgctcacc gcacactgct cactcttcag gaggttgaag gatggaagag
     3481 gttcatctgt ggcgcgggtc gccggagcag cgtgcgctgc gcgcgcaggc ggggaggtcg
     3541 gggcagttcg cctacttcga ccggcagctc ggacaccccg cgtggggcgg catgaaggtg
     3601 ctcgacttcg gcggcaacac gggcaacctg cttgcctccg ccgccgacgc gatccgccac
     3661 gaagattact actgcctcga catcatccgc gacgcgctcg aagtcgggcg cgagaggttc
     3721 ccgcgcgccc acttcgtcca ctacgaccgc tacaactgct ccttcaaccc cga
//
LOCUS       DBNGOH010000870         4801 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_131054, whole genome shotgun sequence.
ACCESSION   DBNGOH010000870 DBNGOH010000000
VERSION     DBNGOH010000870.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4801)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4801)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4801
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_131054"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1382
                     /locus_tag="AC159P_16830"
     CDS             <1..1382
                     /locus_tag="AC159P_16830"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3697161.1"
                     /translation="SPFTIAVVLTGFASVESAIAALRHGAYDYLLKPCNIEELKLTVR
                     RGLEHRRLMLAERAARASLEYINQELERRVAERTGELQRVNEELLEANRAKDVFLATL
                     SHELRTPLTPVLGWVNLLRSGHTNVDTTLLNQGLEAIERNARLQARLVDDLLDISRII
                     SGKLRIEWEPVNLCEVVTAALETVRPEATTRRVTLDVDVPGAPLVVQGAPVRLQQVAW
                     NLLSNAVKFTPDGGRVSLRVWRADGEARVIVEDTGIGIAPEFLPHVFDRFRQADGSTT
                     RQYGGLGLGLAIVRALAELHGGSVRAESEGAGAGARFIFSIPCAVAPEPESEPRAEAE
                     HAPANAPRVLVVDDSTETVQLLHMLFTRRGYPVTTAESAAEALTQAARLRPGVVISDI
                     SMPDVDGYELLTELRRLPGLERVPVIALSGYAMDEDRTRALAAGFNVHLAKPVDPEEL
                     VRVVRRLTS"
     gene            complement(1500..1982)
                     /locus_tag="AC159P_16835"
     CDS             complement(1500..1982)
                     /locus_tag="AC159P_16835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697162.1"
                     /translation="MKKQLTRAFAVFGLMLMSAAFTGAAAQTTRTIFVHVPFEFVVGE
                     KRLPAGDYTIRRILLDSDKTLLIRSIDGRATATVHTSDAGKRAAAPQAKLVFAQYGEQ
                     YFLAQVWTPGAETARALSTSRIRRSLEREWADGATRNGGDAAKVRPEEKTVTIVGGVR
                     "
     gene            2319..4751
                     /locus_tag="AC159P_16840"
     CDS             2319..4751
                     /locus_tag="AC159P_16840"
                     /inference="COORDINATES: protein motif:HMM:NF012698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HIR3697163.1"
                     /translation="MSIRVRAIYEFGPFRLDATERVLARGGETVRLTPKEFETLLALV
                     RNSGHLMTKGELLKEVWPDTFVEEATLAQNIFTLRKALGRGAPEGAGQYIETVPRRGY
                     RFVAGVRELKEETSAAGGEAPGAGAAAANADGVVAAEAEAEPSPSRNGASPAERNGHA
                     AGQAVFSEAGAVSGGAGARTADETAESAASRLRTTEGRGHPVRAAVLISLAVVLVFAG
                     VVLLVFKFVVRRQTAPPSQRQSAFESMQVTRLPVTGDVREAVISPDGKYVAYVANDPG
                     RQSILVRQVSANSNVQQLVAPAAATGYAGLAFSPDAEHLYYVAFKDNGPETALHQVPV
                     LGGAAKKVLDSLHSPITFSPDGRRIAFVRNTRRDTGGAPESSTLFVADADGANARALV
                     THALPEICGLPAWSPRGDTIAYVLGTSESYDFGNVLLGVATLNVADGKETRVTPERWM
                     GISQLAWLPDASGLLLSAAERELSPSQIWRLSLADGEVRRVTNDLNIYLGASMTADAS
                     ALVTVQTDRAPNIWVAPQGDAARAEQITSGTGKLDGIYGVSWAPDGRVVYASVASGNW
                     DIWMMDTDGRNQRQLTVDARSNYGPSVSPDGRHVVFVSNRAGGPFNVWRMEIDGSNPQ
                     QLTFGKGENFAHVTPDGKWVVYATVSFAERNLVWKVPFGGGEPVAITDKPSSWPFVSP
                     DGKWVVCTYYDAPNKPPRLAVVPIDGGPPAKLYDMETSFRANTVWLPDNRGIAYLDNR
                     TGIPNVWLQPTGGGKPVQLTDFKSNGIVSYDWSRDNRLVTTRSVESTGIVLIRNFEGQ
                     QAAGSRQQAAGS"
BASE COUNT          757 a         1715 c         1620 g          709 t
ORIGIN      
        1 cctcgccctt caccatcgcc gtcgtgctca ccggcttcgc ctcggtcgag tcggccatcg
       61 ccgcgctccg ccacggcgcc tacgactacc tcctcaagcc ctgcaacatc gaggagctga
      121 agctgacggt caggcgcggc ctcgaacacc gccgcctgat gctggccgag cgcgccgccc
      181 gcgcctcgct cgaatacatc aaccaggaac tcgaacgccg cgtcgccgag cgcaccggcg
      241 agttgcagcg cgtcaacgaa gaactgctcg aagccaaccg cgcgaaggac gtgtttctcg
      301 ccacgctctc gcacgagctg cggacgcctt tgacgcccgt cctcggctgg gtcaacctgc
      361 tgcgctcggg ccacacgaac gtggacacga cgctcctcaa tcagggcctc gaagccatcg
      421 agcgcaacgc gcgtctacag gcgcggctcg tcgatgacct cttggacatc tcgcgcatca
      481 tctcgggcaa gctccgcatc gagtgggagc cggtcaacct gtgcgaggtg gtgacggcgg
      541 cccttgagac cgtccggccc gaggcgacca cgcgccgtgt gacgctggac gtggacgtgc
      601 ccggcgcgcc gctcgtcgtg cagggcgcgc ccgtgcggct gcaacaggtc gcgtggaacc
      661 ttttgtcgaa cgcggtcaag ttcacgcccg acgggggccg cgtctccctg cgcgtgtggc
      721 gcgccgacgg cgaagcgcgc gtcatcgtgg aggacacggg catcggcatc gcgcccgagt
      781 ttctgccgca cgtcttcgac cgcttccgcc aggccgacgg ctcgaccacg cggcagtacg
      841 gcggcctcgg gttgggtctc gccatcgtgc gcgcgctcgc cgagctgcac ggcggctctg
      901 tgcgcgccga gtcggagggc gcgggcgcgg gcgcgcgctt catcttcagc atcccgtgcg
      961 ccgtcgcgcc cgagccggag tccgagccgc gcgccgaggc ggagcacgcg ccggccaacg
     1021 ccccgcgcgt gctcgtcgtg gacgactcga ccgagacggt gcagctcctg cacatgctct
     1081 tcacgcgccg cggctacccg gtcacgaccg ccgagtccgc cgccgaggcg ctcacgcagg
     1141 ccgcgcgcct gcggcccggc gtcgtcatct ccgacatcag catgcccgac gtggacggct
     1201 acgaactgct caccgagctg cggcggctgc ccggcctcga acgcgtcccc gtcatcgcgc
     1261 tctccggcta cgcgatggac gaggatcgta cccgcgccct cgccgcgggc ttcaacgtcc
     1321 acctcgccaa gcccgtcgat ccggaggaac tcgtccgcgt cgtccgccgc ctcacgagct
     1381 gaccgactcc gccgcgaaaa ccgtcgaaaa aaactttggg gccggggcga gaccgcagtc
     1441 cgccccggcc cctctcacgc gccggaggcc cactcgcggc gcatgattcg tccgaacgtt
     1501 cagcgcacgc cgccgacgat ggtgacggtt ttttcctcgg ggcgcacttt ggccgcgtcg
     1561 ccgccgttgc gggttgcgcc gtcggcccac tcgcgttcga ggctgcgccg aatgcgcgac
     1621 gtggagagcg cgcgggccgt ctcggcgccg ggcgtccaga cctgggcgag gaaatactgc
     1681 tcgccgtact gcgcgaagac cagtttcgcc tgcggggcgg cggcccgctt gccggcatcg
     1741 ctggtgtgga cggtggccgt ggcgcgtccg tcgatgctgc ggatgagcag cgtcttatcg
     1801 gagtcgagca ggatgcggcg gatggtgtag tcgcccgcgg gcagccgctt ctcgccgacg
     1861 acgaactcga acgggacgtg gacgaagatc gtgcgcgtgg tctgcgccgc cgcgcccgtg
     1921 aaggccgcgg acatcaacat gaggccgaac accgcgaacg ctctggtcag ttgctttttc
     1981 atcttccctc tccttattat ctttcccttg gccgccggtc gaaatttgcc cgccgcggct
     2041 cttgtttttt tcgcggcgac ggattccgcc gccgccggcc cccaccgacg ccccatgcat
     2101 gtctccgggc gcatcgctca aactaaaaac agcgagcggg agacaaccgg agttcgtacg
     2161 aagagcatcg gctacttcgc tggtgcaatg tacgcgcgga gccgccgggg ggtctttaaa
     2221 gttgggtcaa atgcgggcca aaaacccttg aaaaaagctt caaagagtta tcaaagacgt
     2281 gtggagggca gcccggtcgg gttgccgggc ggtcgaagat gagtattcga gtgagggcaa
     2341 tttatgagtt cggcccgttc cgtctggacg cgaccgagcg ggtgctcgcg cgcggcggcg
     2401 agacggtgcg cctgacgccg aaggagttcg agacactgct ggcgctcgtc cgcaacagcg
     2461 gccacctgat gacgaagggc gagctgctga aagaggtctg gccggacacg ttcgtcgaag
     2521 aggcgacgct cgcgcagaat atcttcacgc tgcgcaaggc gctcggcagg ggcgcgccgg
     2581 agggcgcggg gcagtacatc gagaccgtcc cccgccgcgg ctaccgcttc gtcgcgggcg
     2641 tgcgcgagct gaaggaagaa acttccgcgg cgggcggcga ggcgccgggc gcgggcgccg
     2701 ccgcagcgaa cgccgacggc gtcgtcgcgg cggaggccga ggccgagccg tcgccgtctc
     2761 gaaacggggc atcgcccgcc gagcgcaacg gccacgccgc cggtcaagcg gtgttttccg
     2821 aagcgggcgc ggtttccggc ggggcgggcg cgcgcacggc ggacgagacg gcggaatccg
     2881 cggcgtcgcg gcttcgcacg acggaagggc gcgggcatcc cgtgcgcgca gccgtgctga
     2941 tctcgctggc agtggtgctc gtgttcgcgg gtgtggtgct gctcgtcttc aagttcgtgg
     3001 tgcgcaggca gacggcgccc ccgtcgcaga ggcagtcggc cttcgagtcg atgcaggtga
     3061 cgcggctgcc cgtgacgggc gacgtgcgcg aggccgtcat ctcgcccgac ggcaagtacg
     3121 tcgcctacgt cgccaacgac cccgggcggc agagcatcct cgtccggcag gtctcggcca
     3181 acagcaacgt ccagcagctc gtcgcgcccg ccgccgcgac cggctacgcc ggcctcgcct
     3241 tctcgcccga cgccgaacac ctctactacg tcgcgttcaa ggacaacggc cccgagacgg
     3301 cgctgcatca ggtgccggtg ctcggcggcg cggcgaagaa ggtgctcgac agcctccaca
     3361 gccccatcac cttctcgccc gacggcaggc gcatcgcctt cgtccgcaac acgcggcggg
     3421 acacgggcgg cgcgcccgaa agctccacgc tcttcgtcgc cgacgccgac ggcgcgaacg
     3481 cacgcgcgct ggtgacgcac gcgctgccgg aaatctgcgg gctgcccgcg tggtcgcccc
     3541 gcggcgacac gattgcctac gtgctcggca cgtcggagag ctacgacttc ggcaacgtgc
     3601 tgctcggcgt ggcgacgctc aacgtcgcgg acggcaagga gacgcgcgtc acgcccgagc
     3661 gctggatggg catcagccag ctcgcgtggc tgccggacgc gagcggcctg ctgctgagcg
     3721 cggcggagcg cgaactcagc ccctcgcaaa tctggcggct ctctctcgcg gacggcgagg
     3781 tgcgccgcgt cacgaacgac ctcaacatct acctcggcgc gagcatgacg gcggacgcct
     3841 cggcgctcgt caccgtgcag accgaccgcg cgcccaacat ctgggtcgcg ccgcagggcg
     3901 acgccgcgcg cgccgagcag atcacctccg gcacgggcaa gctcgacggc atctacggcg
     3961 tctcgtgggc gccggacggg cgcgtcgtct acgcctcggt ggcgagcggc aactgggaca
     4021 tctggatgat ggacaccgac gggcggaacc agcggcagtt gaccgtggac gcgcgctcga
     4081 actacggccc ttcggtctcg cccgacgggc ggcacgtcgt cttcgtctcg aaccgcgcgg
     4141 gcggcccctt caatgtctgg cgcatggaga tcgacggctc gaacccgcag cagctcacct
     4201 tcggcaaggg tgagaatttc gcgcacgtca cgcccgacgg caagtgggtc gtctacgcca
     4261 ccgtcagctt cgccgagcgc aatctcgtct ggaaggtgcc gttcggcggc ggcgagccgg
     4321 tggccatcac cgacaagccc tcttcgtggc ccttcgtctc gcccgacggc aagtgggtcg
     4381 tctgcaccta ctacgacgcg ccgaacaagc cgccccggct cgcggtcgtc cccatcgacg
     4441 gcgggccgcc cgccaaactc tacgacatgg agaccagctt ccgcgccaac accgtctggc
     4501 tcccggacaa ccgcggcatc gcctatctcg acaaccgcac cggcatcccc aacgtctggc
     4561 tccaaccgac gggcggcggc aagcccgtgc agctcaccga cttcaaatcc aacggcatcg
     4621 tcagctacga ctggtcgcgc gacaaccgcc tcgtcaccac ccgcagcgtc gaatcgacgg
     4681 gcatcgtcct catccgtaac ttcgaaggac agcaggcagc aggcagcagg cagcaggcag
     4741 caggcagtta aagcaagagg cttgagaggt aaagtccaga gcctcgatct cggatacttg
     4801 a
//
LOCUS       DBNGOH010000871         2290 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3613078, whole genome shotgun sequence.
ACCESSION   DBNGOH010000871 DBNGOH010000000
VERSION     DBNGOH010000871.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2290)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2290)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2290
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3613078"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..209
                     /locus_tag="AC159P_16845"
     CDS             <1..209
                     /locus_tag="AC159P_16845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010253214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697164.1"
                     /translation="RAATVRAIVLPGNAEPLLGVIPMEDMDVVVDPKQQRLVVNPETP
                     YIVSRYVKRHAVACDAASQAVQAV"
     gene            219..1247
                     /locus_tag="AC159P_16850"
     CDS             219..1247
                     /locus_tag="AC159P_16850"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HIR3697165.1"
                     /translation="MSTATRKEHKSNRPERGGMATMVEAIRQGLWEEMERDPSVFLIG
                     EDVGAYGGAFKVTDGLLDEFGEGRVIDTPISEAAIVGAACGAALMGMRPVAEFQFIDF
                     ISPAFDMLTNYAAKCRYRWGAGLPTVFRGPCGAGVHSGPFHSLNAESFFMNTAGLKMV
                     EPSTAYDAKGLIKAAIRDPDPVLFFEHKKLYRLPRLREELPAEDYVVPIGKARVRREG
                     RDLSIITFGAQVLNALDAAEELEKEGLDVEVVDLRTLAPMDREAILSTAKKTSKVLIL
                     HEASRTGGIGGEIAATICEEAFEWLDAPVVRVAAIDTPVPYSPPLEEYHLPQVRDVLA
                     AARKLAAY"
     gene            1409..1684
                     /locus_tag="AC159P_16855"
     CDS             1409..1684
                     /locus_tag="AC159P_16855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009048626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4242 domain-containing protein"
                     /protein_id="HIR3697166.1"
                     /translation="MPKYLIERSVPGAGSLSISTLREVSQKSCNVLQEMGPQIQWVHS
                     YVTGDKLYCVYIAPDEESVREHARRGEFPADSVEEIKTMIDPTTAEL"
     gene            1887..>2290
                     /locus_tag="AC159P_16860"
     CDS             1887..>2290
                     /locus_tag="AC159P_16860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013874420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HIR3697167.1"
                     /translation="MATEVVMPQMGESIAEGTITRWLKKVGEKVERDEPLFEISTDKV
                     DAEIPSPAAGTLQEIKFKEGDTVEVNVVVALIGDGAAAAAPSGGATEAAPPPAASAPP
                     APASQAAVSAPAPPPPPAQTPAPPAPQQQAAPP"
BASE COUNT          418 a          705 c          819 g          348 t
ORIGIN      
        1 accgcgcagc gacggtgcgg gcgatagttc tccccggcaa cgccgagcct ctgctcggag
       61 tcattccgat ggaggacatg gacgtagtgg ttgaccccaa acaacagcgc ctcgtcgtca
      121 acccggagac cccgtacatc gtcagtagat acgtgaagcg acacgcggtc gcgtgcgacg
      181 cggcgtctca agcggtgcaa gcagtctgaa ttcttacaat gtcaacagcg actagaaaag
      241 agcacaaatc gaaccgcccg gagcgcggcg ggatggcgac gatggtcgag gccatcagac
      301 aggggctgtg ggaggagatg gagcgcgacc cgtcggtgtt cctcatcggc gaggacgtgg
      361 gggcgtacgg cggcgcgttc aaggtgacgg acgggctgct ggacgagttc ggcgaggggc
      421 gcgtcatcga cacgccgatc tcggaggcgg cgattgtcgg ggcggcgtgc ggtgcggcgt
      481 tgatggggat gcggcccgtc gccgagttcc agttcatcga tttcatctcg cccgccttcg
      541 acatgctgac gaactacgcg gcgaagtgcc gctaccgctg gggcgcgggg ctgccgaccg
      601 tcttccgcgg gccgtgcggc gcgggcgtcc actcggggcc gttccactct ctgaacgccg
      661 agagcttctt catgaacacc gcggggctga agatggtcga gccttcgacg gcctatgacg
      721 ccaaaggttt aatcaaggcc gccatccgcg accccgaccc cgtgctcttc ttcgagcaca
      781 agaagctcta ccgcctgccg cgcctgcgcg aggagctgcc cgcggaggac tacgtcgtgc
      841 ccatcggcaa ggcgcgcgtg cgccgcgagg ggcgcgattt gtcgatcatc acgttcggcg
      901 cgcaggtttt gaacgcgctc gacgcggcgg aggagctgga gaaggagggc ttggacgtgg
      961 aggtggtaga cctgcgcacg ctggccccga tggacaggga ggccatcctc tcgacggcga
     1021 agaagacgag caaggtgttg atcctgcacg aggcttcgcg cacgggcggc atcggcggcg
     1081 agatcgcggc gaccatctgc gaggaggcgt tcgagtggtt ggacgcgccg gtcgtgcgcg
     1141 tcgcggccat cgacacgccc gtcccttact cgccgccgct cgaagagtac cacctgccgc
     1201 aggtcaggga cgtgctcgcg gcggcgcgca agctcgcggc gtattgaaga agcaggcagc
     1261 gggcagcagg cagcaggcag caggcagtta aaaacacttt gagatttacg agttcaacat
     1321 ggcatctcga acctcaaagc cctaatttga attggtattt cttgactgcc cgctgcccgc
     1381 tgcgattgcg attgaaagga gcaaaaacat gccgaagtat ctgatcgagc ggagtgtgcc
     1441 gggcgcgggg agtttgtcca tcagcacgtt gcgcgaggtg tcgcagaagt cgtgcaacgt
     1501 gctgcaggag atggggccgc agattcagtg ggtccacagc tacgtgacgg gcgacaagct
     1561 gtactgcgtc tacatcgcgc cggacgagga gtccgtgcgc gagcacgcgc ggcggggcga
     1621 gttcccggcg gacagcgtcg aggagatcaa gacgatgatc gacccgacga ccgcggaact
     1681 ctgacggcgg cgggccgtcg gcgtgtgcgc gccgaagggg tggccgtgcg gcgcaaggcg
     1741 cggcggcgag gtgccagcga taaataattt ccggggcggc gtgtcgcctc gtctcttctg
     1801 ctgctcggac tgacggcggc gggcgccccc cccgccgcaa gtccggtaag atgaaggcca
     1861 aagaataaac ggtaggtgga gaagatatgg cgacagaagt ggtgatgccg cagatgggcg
     1921 agtccatcgc ggaggggacg attacgcggt ggctgaagaa ggtcggcgag aaggtcgagc
     1981 gcgacgagcc gctgttcgag atttcgaccg acaaggttga cgcggagatt ccttcgcccg
     2041 ccgcggggac gcttcaggag atcaagttca aggagggcga cacggtcgag gtcaacgtcg
     2101 tcgtcgcgct catcggcgac ggcgccgccg ccgccgcccc ttcgggcgga gcgaccgaag
     2161 ccgcgccgcc gcccgccgcg agtgcgccgc ccgcgcccgc gagccaggcc gccgtctccg
     2221 cgcccgcgcc cccgcctcct ccggcgcaga cgcccgcgcc ccccgcgcca caacaacagg
     2281 ccgcgccgcc
//
LOCUS       DBNGOH010000872         2328 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3064817, whole genome shotgun sequence.
ACCESSION   DBNGOH010000872 DBNGOH010000000
VERSION     DBNGOH010000872.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2328)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2328)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2328
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3064817"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1334
                     /locus_tag="AC159P_16865"
     CDS             <1..1334
                     /locus_tag="AC159P_16865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697168.1"
                     /translation="DSKFFLIRVRPLRLFFWVPFLLALGWLIYWLAKRFDLLCDRKPV
                     IWPDKRPYSLSAVQAAWWFFIILAMFIFIWLVTNQYNFSETALVLLGIGAGTALGATV
                     IDSNKQGRGQADAGAGDDDALKVLLDNKQRLEATLTERAARLAAAKRRQKGLEAQQLS
                     DALSADARQKIQAGLDETVAEIKALQPEFDDAKGKYDEVIATIKTSFPKAIGPGRESF
                     LLDILSDEGGISFHRLQMLVWSLVLGIFFVASALGVLAMPNFSATLLGLMGLSAGTYL
                     GFKIPEDPGVPSLPQSGGGGNGGDAEGAAGATGATGAGTTTGTTDTSTTTDTGTTTDT
                     TDVTDTTGATNTTGATDTTGATDTTGATDATDATGAGGGTGTTDTAADTGLGDPGTEG
                     SGEQSDAATAGETGGDSLAEGEQEESAADESAASDTGEEEASSPGGGGAAG"
     gene            complement(1390..1749)
                     /locus_tag="AC159P_16870"
     CDS             complement(1390..1749)
                     /locus_tag="AC159P_16870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004272295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mpo1-like protein"
                     /protein_id="HIR3697169.1"
                     /translation="MKEREISTFEEFWPFYVREHSRPATRALHAAGTITSTALVVALV
                     LAGRWRWLPLALVVGYAFAWAAHFFVEHNRPATFKYPLWSFMADYKMVALMLTGRMGA
                     EVARARDERAASGGSVA"
     gene            complement(1819..>2328)
                     /locus_tag="AC159P_16875"
     CDS             complement(1819..>2328)
                     /locus_tag="AC159P_16875"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007914416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HIR3697170.1"
                     /translation="DWEDPASEGEKVLGNIRRLLSKAREAGAPVVYVQHDGEEGGRLS
                     VGSKGWEIHPAIAPAEGEPVVRKRASDSFYETTLREDLEARGVRHLVVVGCRTQYCVD
                     TTCRSATAHGFDVTLASDAHTTAADVLTVPQIVAHHNSTLDDFGTDRHVVTVKDSAEI
                     DFENQKYEV"
BASE COUNT          394 a          770 c          818 g          346 t
ORIGIN      
        1 atgattcgaa gttcttcctc atccgcgtcc gccccttgcg cctgttcttc tgggttcctt
       61 tcctgctggc tctgggttgg ctcatttact ggctggcgaa gcgtttcgac ctgttgtgcg
      121 accgcaagcc ggtcatctgg ccggacaaga gaccctacag cctgagcgcc gtgcaggccg
      181 cgtggtggtt cttcatcatc ctcgccatgt tcatcttcat ctggctcgtg acgaaccagt
      241 acaacttctc cgagacggcg ctcgtgctgc tcggcatcgg cgccggcacc gcgctcggcg
      301 cgaccgtcat cgactcgaac aaacaggggc ggggccaggc cgacgccggg gccggcgacg
      361 acgacgcgct caaggtgctg ctcgacaaca agcagcggct cgaagccacg ctgaccgagc
      421 gggctgcccg gttggccgct gcgaagaggc gccagaaggg gttggaggcg caacagttgt
      481 cggacgcact gtccgccgac gcgcggcaaa agatacaggc cggcctcgac gagacggttg
      541 ccgagatcaa agcgctacag ccggagttcg acgacgcgaa gggcaagtac gacgaggtca
      601 tcgcgacgat caagaccagc ttcccgaagg ccatcgggcc ggggcgcgag agctttctgc
      661 tcgacatcct ctcggacgag gggggcatca gcttccaccg tctccagatg ctcgtctggt
      721 cgctggtgct cggcatcttc ttcgtcgcct cggcgctcgg cgtgctggcg atgcccaact
      781 tcagcgccac gctgctcggt ctgatgggcc tcagcgccgg cacctacctc ggcttcaaga
      841 tccctgaaga ccccggtgtg ccctcgctcc cgcagagcgg cggcggcggc aacggtggtg
      901 acgcggaggg cgcggcgggc gcgaccggcg cgaccggcgc gggcacgacg accggcacga
      961 ccgacacaag caccacgacc gacacaggca ccacgaccga cacgactgat gtgaccgaca
     1021 cgaccggcgc aaccaacacg accggcgcga ccgacacgac cggcgcgacc gatacgaccg
     1081 gcgcaaccga cgcgacggac gcgaccgggg ccgggggcgg aaccgggacg acggacacgg
     1141 cggctgacac cggtctcggc gatcccggca cggaaggttc gggcgagcag tcggacgcgg
     1201 ccacggcggg tgagacggga ggagactcgt tggccgaagg cgagcaggaa gagtcagcgg
     1261 cggacgaaag cgctgcgtcc gatacgggcg aagaggaagc ctccagtccg ggcggcggcg
     1321 gcgcggcggg ctaagcgagc aggggcgatc agagggcgcg ggtgcgttgg caggcggcgg
     1381 ctcggctcgc tacgcgacag agccgccgct cgccgcgcgc tcgtcgcgcg cgcgcgcgac
     1441 ctccgcgccc atccggcccg tgagcatgag ggcgaccatc ttgtagtcgg ccatgaagga
     1501 ccagagcggg tacttgaagg tggcgggccg gttgtgctcg acgaagaagt gcgccgccca
     1561 cgcgaaggcg tagccgacga cgagcgccaa aggcagccac cgccagcgcc ccgcgaggac
     1621 gagcgcgacg acgagcgccg tgctcgtgat cgtgcccgcc gcgtgcagcg cccgcgtcgc
     1681 cgggcggctg tgctcgcgca cgtagaaggg ccagaactcc tcgaacgtgc tgatctcccg
     1741 ttccttcacg acaattgccc tccgcatgaa agaaagtcag aagtcagaag tatgaacgaa
     1801 aagcggcctg cctgtcgttc atacttcata cttctgattt tcgaagtcta tctccgccga
     1861 gtccttgacg gtgacgacgt ggcggtcggt gccgaagtcg tcgagggtcg agttgtggtg
     1921 ggcgacgatc tgcgggacgg tgagcacgtc ggcggcggtc gtgtgcgcgt ccgacgcgag
     1981 cgtcacgtcg aagccgtgcg cggtcgcgct gcggcaggtc gtgtccacgc agtattgcgt
     2041 gcggcagccg acgacgacga ggtgacggac gccgcgcgct tcgaggtctt cgcgcagggt
     2101 cgtctcgtag aacgagtcgg aggcgcgctt gcggacgacg ggttcgccct cggcgggcgc
     2161 gatggccggg tgtatctccc agcccttcga gccgaccgag aggcgtccgc cctcttcgcc
     2221 gtcgtgctgg acatagacga cgggcgcgcc cgcctcgcgc gccttcgaga gcaggcggcg
     2281 gatgttcccc agcaccttct cgccttcgct cgcagggtct tcccaatc
//
LOCUS       DBNGOH010000873         5446 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4530286, whole genome shotgun sequence.
ACCESSION   DBNGOH010000873 DBNGOH010000000
VERSION     DBNGOH010000873.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5446)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5446)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5446
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4530286"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..518)
                     /locus_tag="AC159P_16880"
     CDS             complement(<1..518)
                     /locus_tag="AC159P_16880"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR3697171.1"
                     /translation="MIRVEKVEKSVGELRILKGISFEVAPQTVLGIIGGSGSGKTTLL
                     RCINGFERLTGGAVECNGVRLEAALGDSEYQKRVKLVRREVGMVFQHLYLFPHLTALA
                     NVTEAPVHVSRAPRGRAEEEALALLESVGLRAKASRYPEHLSGGEQQRVAIARALAMR
                     PDVLLFDEPTSAL"
     gene            complement(637..2154)
                     /locus_tag="AC159P_16885"
     CDS             complement(637..2154)
                     /locus_tag="AC159P_16885"
                     /inference="COORDINATES: protein motif:HMM:NF012707.7"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HIR3697172.1"
                     /translation="MKKLLSGLLKFVSTVLLTHCIVTTAAAQQRELRWGADPSGGAPY
                     VFSDPAHPDTYIGYEKEMVDALAAAMGRRPVFVPTDWQTIVSSLQRGGFDVIVNGLEP
                     TADRERQILFSKPYYIFQFQLTVRKNETRIDSLEDCRKRLVGTLGNTAASRLLEKEGI
                     PFRGYEDPVGAYRDLELGRLDAVLMDVPMEMFYARPNARLKPAGGPFHRGLYIIGLRK
                     GDDALKAEVDAAIQKIIDDGTLERILRKWSLWNEAQAELRQQAAPPNAAGAEVQPTVA
                     YDLTSTTFNWGEALLRLGRAALVTVGLSFGAMLLALSLGLALAIGQTHGPRWLQAVST
                     VYVEFFRGTPVLVQLLFLYFGLPRLGVAMPGWLTAMVGLGLNYAAYESQVYRAALGAI
                     PRGQWEAAGALGMSPVLAFRRIILPQAFRIALPPMTNDFVSLFKDTSVAFAISVWELA
                     TAYRELANASGEFLLLGVAVSLFYLAMSLPLAHLARRLENRLQRRKEEAQAAGAA"
     gene            complement(2363..3268)
                     /locus_tag="AC159P_16890"
     CDS             complement(2363..3268)
                     /locus_tag="AC159P_16890"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR3697173.1"
                     /translation="MFFTDSYRALRRGERRLALRHAALSVLLLTVIGSPVGFLMLRRI
                     EKAITFHPDRTPVRGTWAVPEGAEEVWFGVATGARLHGWFFRAKGQQPARATVVYFHG
                     NGGNVSYLDWFGDAYARRGFDVLLFDYRGYGRSEGEVSDERGIYEDADAAYDFVVREH
                     GARPERLVLHGQSLGTTAAADLAARKVCGAVILESGLSSAGDMAGVVMPWLPRFVRAR
                     TRNKFDSVGKLGGVRCPVLVIHGDLDRTIPVAQGRALYEAAPEPKRLLVVEGAGHNDL
                     SVVAGEKYMNTVAEFIESALALKSR"
     gene            complement(3472..4713)
                     /locus_tag="AC159P_16895"
     CDS             complement(3472..4713)
                     /locus_tag="AC159P_16895"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 metallopeptidase family protein"
                     /protein_id="HIR3697174.1"
                     /translation="MTVGGDAAARQQRIARAAEGMRERLVAQRRDIHMHPELSNREER
                     TARVVAERLKALGLTDIKTGVARHGVTALLVGAKPGPVVAVRADMDALPINETLDVPY
                     KSKNPGVKHACGHDVHTTVALGVAELLAGMKEEIAGTVKFIFQPAEEGAPAGEEGGAR
                     LMVKEGAMENPRPAAIFGLHTLPSIEAGQIAYHSGPAMASSDKIYVTIRGRGAHGAQP
                     HNGVDAIVVAAEAITALQHIRSRRIDPQEPLVITLGTIHGGARNNIIAEEVKMEGTMR
                     TLSEEVRQRAQTLMRETLLHVTAAYGATFELKFDEQTLVTYNEPALVEETLPTIRRVV
                     GDRNTVAIKPFMPAEDFSYFQKVSPGFYYFLGVGNRAKGITAGWHTPEFDVDEASLVV
                     GVNVMSNVLLDYLERRGAEKK"
     gene            complement(4923..>5446)
                     /locus_tag="AC159P_16900"
     CDS             complement(4923..>5446)
                     /locus_tag="AC159P_16900"
                     /inference="COORDINATES: protein motif:HMM:NF039409.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6328 family protein"
                     /protein_id="HIR3697175.1"
                     /translation="RPEGERGKKEEGGEVRDKIKHVLTEARMALPGAQALLGFQLIAV
                     LMESFERLPATSKYVHLAGLVCIALSIVLLMTPAAYHRIVEKGEETEHFHRFAGRLVV
                     AALVPLALGICGGVYVVVQKVLASTFIAVVASVVALAVFYELWFGLTVYRRIQRDHEK
                     PKTGAPGLFGASQ"
BASE COUNT          880 a         1826 c         1824 g          916 t
ORIGIN      
        1 agggcgctgg tcggctcgtc gaagaggagc acgtcggggc gcatcgccag cgcgcgcgcg
       61 atggccacgc gctgctgctc gccgcccgag aggtgttcgg ggtagcggct ggctttggcg
      121 cgcaggccga cggattcgag cagcgcgagc gcctcctctt cggcccgccc gcgcggggcg
      181 cgcgagacgt ggacgggggc ctccgtgacg ttggccagcg cggtcaggtg cgggaacagg
      241 tagaggtgct ggaagaccat ccccacctcg cgccgcacga gcttcacgcg cttctggtat
      301 tcggagtcgc cgagggccgc ttcgagcctc acgccgttgc actcgacggc gccgcccgtc
      361 agtcgctcga agccgttgat gcagcggagc agcgtcgtct tgccgctgcc cgagccgccg
      421 atgatgccga gcacggtctg cggcgcgacc tcgaagctga tgcccttcag gatgcgcagc
      481 tcgccgacgc tcttctcgac cttctcgacc cgaatcatgt cgtcacctcc acattgcgga
      541 ttgcggattg aatgaccagc gatagttgga ctcctaaaac caagcattaa ccttgagcag
      601 cacgctaatc cgcaatccgc aatccgcaat ccgcaatcaa gccgcccccg cggcctgcgc
      661 ctcttccttc cgccgttgca ggcgattctc aagccgccgc gcgagatgcg ccagcggcaa
      721 actcatcgcc agataaaaaa gcgagaccgc gacgccgagc agcagaaact cgcccgacgc
      781 gttggccagc tcgcggtacg cggtcgccag ctcccacacc gagatggcga aggcgaccga
      841 cgtgtctttg aagagcgaga cgaagtcgtt ggtcatcggc ggcagcgcga tgcggaacgc
      901 ctgcggcagg atgatgcggc gaaaggcgag caccggactc atgccgagcg cgcccgccgc
      961 ctcccactgc ccgcgcggga tggcgccgag cgcggcgcgg tagacctgcg actcgtacgc
     1021 ggcgtagttc agcccgagtc cgaccatcgc cgtcagccag cccggcatcg ccaccccgag
     1081 tcgcggcagg ccgaagtaga gaaagagcaa ctggacgagc acgggcgtgc cgcggaagaa
     1141 ctcgacgtag accgtggaca cggcttgcag ccagcgcggg ccgtgcgtct gcccgatggc
     1201 gagcgcgagt ccgagcgaca gcgccagcag catcgccccg aacgagaggc ccacggtgac
     1261 gagcgccgcg cgacccagac gcagcagcgc ctcgccccag ttgaacgtgg tcgaggtgag
     1321 gtcgtaggcg accgtcggtt ggacctccgc gcccgccgcg ttcggaggcg cggcctgttg
     1381 tctcagctcg gcctgcgcct cgttccacag gctccacttc cgcaggatgc gttcgagcgt
     1441 gccgtcgtcg atgattttct gtatcgccgc gtccacttcg gccttgagcg cgtcgtcgcc
     1501 tttgcgcagg ccgatgatgt agaggccgcg gtggaacggc ccgcccgcgg gcttgagccg
     1561 cgcgttcggg cgcgcgtaga acatctccat cggcacgtcc atcagcaccg cgtcgaggcg
     1621 cccgagttcg aggtcgcggt acgcgcccac cgggtcttcg tagccgcgaa aagggatgcc
     1681 ctccttctcc aacaggcgcg acgcggcggt gttgccgagc gtgccgacca gacgcttgcg
     1741 gcagtcttcg agcgagtcga tgcgcgtctc gttttttctg acggtgagct gaaactggaa
     1801 gatgtagtaa ggcttgctga agaggatttg gcgttcgcgg tcggcggtcg gctccagccc
     1861 gttgacgatc acgtcgaagc cgccgcgctg caacgaagag acgatggtct gccagtcggt
     1921 cggcacgaac acgggccgcc ggcccatcgc cgccgccagc gcgtccacca tctccttctc
     1981 gtagccgatg tacgtgtccg ggtgcgccgg gtcggagaag acgtaaggcg cgccgccgct
     2041 cgggtccgcg ccccagcgca gctcgcgctg ctgcgccgcg gcggtcgtga cgatgcaatg
     2101 tgtgaggaga actgtcgaga cgaatttcag aagaccgcta agaagttttt tcacccgact
     2161 ctccccgacg aagaatgaag acggacacgc tgtttgtcgt tcgagaaact taagggccgt
     2221 ttcaagacct caaaggcggc gatgatagta ggggcagggc ttgtctctgc ccgcgcctgc
     2281 atgttcactc aacgttgagt ccggcggatg ttgacgggca gggacaagcc ctgcccctac
     2341 agtcttacaa gtaaccgcac gcctaacgag acttcaacgc cagcgcgctc tcgatgaact
     2401 ccgcgaccgt gttcatgtac ttctcgccgg cgacgacgga caggtcgttg tggcccgcgc
     2461 cctcgacgac gagcagccgc ttcggctcgg gcgccgcttc gtagagcgcg cgcccctgcg
     2521 cgacgggtat cgtgcggtcg aggtcgccgt ggatgacgag gacggggcag cgcacgccgc
     2581 cgagcttgcc gacggagtcg aacttgttgc gcgtccgcgc gcggacgaag cgcggcagcc
     2641 acggcatgac gacgcccgcc atgtcgcctg ccgaactcaa gcccgattcg aggatgacgg
     2701 cgccgcacac cttgcgcgcg gcgaggtcgg cggcggcggt cgtccccaga gactgcccgt
     2761 ggagcacgag tcgttcgggc cgcgcgccgt gctctctgac cacgaaatcg taggcggcgt
     2821 cggcgtcctc gtaaatcccg cgctcgtccg agacctcgcc ctcgctacgc ccgtagccgc
     2881 ggtagtcgaa gagcagcacg tcgaagccgc gccgcgcgta agcgtcgccg aaccaatcga
     2941 ggtagctcac gttgccgccg ttgccgtgga agtagacgac ggtcgcgcgc gcgggctgct
     3001 gccccttcgc gcggaagaac cagccgtgca gccgcgcgcc cgtggcgacg ccgaaccaca
     3061 cctcctccgc gccctcgggc accgcccaag tgccgcgcac cggggtgcgg tcggggtgaa
     3121 acgtgatcgc cttctcgatc ctgcggagca taaggaagcc gaccggcgag ccgatcaccg
     3181 tcaggagcag cacggacagt gcggcgtgac ggagtgcgag ccgccgttcg ccgcgccgga
     3241 gcgcgcggta agagtccgtg aagaacatag gtttcgcggt gcgcgtctca tcgttcgtcg
     3301 agttatgacc ggaggcggct tttaacacag agggcacgga ggagacacgg agggcgcaga
     3361 gaactcatct ctctgtgtcc tccgtgtctt ctccgtgccc tctgtgttat tgatgaaccc
     3421 tcacctcact caacctacat cgagaccggt tcacacgccg ccccgcgccg ttcacttttt
     3481 ctccgcgccg cgccgttcca gatagtcgag caggacgttc gacatcacgt tgacgccgac
     3541 caccagactc gcctcgtcca cgtcgaactc gggcgtgtgc cacccggcgg tgatgccctt
     3601 cgcgcggttg ccgacgccga ggaagtagta gaagccgggc gagaccttct ggaagtagga
     3661 gaagtcctcg gcgggcatga agggcttgat cgcaaccgtg ttccggtcgc cgacgacgcg
     3721 gcggatcgtg ggcagcgtct cctcgaccag cgccggctcg ttgtaggtga cgagcgtctg
     3781 ctcgtcgaac ttaagctcga aggtcgcgcc gtaggcggcg gtgacgtgta gcagcgtctc
     3841 cctcatcaac gtctgcgcgc gctgccgcac ctcctcgctg agcgtgcgca tcgtgccttc
     3901 catcttcacc tcttcggcga tgatgttgtt gcgcgcgccg ccgtggatgg tgccgagcgt
     3961 gatgaccagc ggctcctgcg ggtcgatgcg gcggctgcgg atgtgctgca aggcggtgat
     4021 ggcttcggcg gcgacgacga tggcgtccac gccgttgtgc ggctgcgcgc cgtgcgcccc
     4081 tctgccgcgg atggtgacgt agattttgtc ggacgaggcc atcgcggggc cggagtggta
     4141 ggcgatctgc ccggcctcga tgctcggcag cgtgtgcagg ccgaagatgg ccgcgggccg
     4201 cgggttttcc atcgcgccct ctttgaccat caaacgcgcg ccgccctcct cgcccgcggg
     4261 cgcgccctcc tccgccggct ggaagatgaa cttgacggtg cccgcgatct cttccttcat
     4321 cccggcgagc agttccgcca cgccgagcgc gaccgtcgtg tgcacgtcgt gcccgcacgc
     4381 gtgcttgacg ccggggttct tcgatttgta cggcacgtcc agcgtctcgt tgatcggcag
     4441 cgcgtccatg tcggcgcgca cggcgacgac ggggccgggc ttcgcgccga ccaggagcgc
     4501 cgtcacgccg tgccgcgcga cgcccgtttt gatgtccgtg aggccgagcg ccttcagccg
     4561 ttcggcgacg acgcgcgcgg tgcgctcctc gcggttcgac agctccgggt gcatgtgtat
     4621 gtcgcggcgc tgcgcgacga gtctctcgcg catgccttcc gccgcgcgcg cgattctttg
     4681 ctggcgggcc gccgcgtcgc cgccgaccgt cataccggca acgagcttct gtccgttgac
     4741 agtcgcgggc gcggcgagca gtacgataat taagagaagc gagagtggaa aagatttacc
     4801 acgaaggcac gaaggcacga aggaacacaa aggaacgcgc gcgcgaacgt ctacgccttc
     4861 tcgtttcttc tccttcgtgc cttcgtgcct tcgtggttga tttaatcgct tgagcagatg
     4921 gttcattggc tggctccgaa aaggccgggg gcgccggtct tgggtttttc gtggtcgcgc
     4981 tggatgcggc ggtagacggt gaggccgaac cagagttcgt agaagacggc gagcgcgacg
     5041 accgaggcga cgacggcgat gaacgtcgag gcgagcacct tctgcacgac gacgtagacg
     5101 ccgccgcaga tgccgagcgc gagcggcacc agcgccgcga ccaccagcct gccggcgaag
     5161 cggtggaagt gctcggtctc ttcgcccttc tcgacgatgc ggtggtaggc ggcgggcgtc
     5221 atcagcagca cgatggagag cgcgatgcac acgaggccgg cgaggtggac gtacttcgag
     5281 gtcgccggca gccgctcgaa gctctccatc aggacggcga tgagctggaa gcccagcagc
     5341 gcctgcgcgc ccggcagcgc catccgcgcc tcggtcagca cgtgcttgat cttgtccctg
     5401 acctcgccgc cctcttcctt cttccctctc tctccctccg gcctca
//
LOCUS       DBNGOH010000874         4309 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3613481, whole genome shotgun sequence.
ACCESSION   DBNGOH010000874 DBNGOH010000000
VERSION     DBNGOH010000874.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4309)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4309)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4309
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3613481"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /locus_tag="AC159P_16905"
     CDS             <1..255
                     /locus_tag="AC159P_16905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012494251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3697176.1"
                     /translation="GHVVLFEPHERKVFVGDCLFAGSIGRTDLPGGSTEQLLDSITNK
                     ILPLGDDVVVYSGHGPETTIGHERLTNPFLTGGYKLGRYL"
     gene            complement(311..523)
                     /locus_tag="AC159P_16910"
     CDS             complement(311..523)
                     /locus_tag="AC159P_16910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697177.1"
                     /translation="MSKEKPKVPNQINEGTGEFDARFMLWRTFCAEHGVPVETLPSDL
                     QGDVKEKWEKLKNERLHKPAEEGQKP"
     gene            complement(678..2585)
                     /locus_tag="AC159P_16915"
     CDS             complement(678..2585)
                     /locus_tag="AC159P_16915"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015645 - fatty acid ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-dependent synthetase/ligase"
                     /protein_id="HIR3697178.1"
                     /translation="MTTTNTTREIQSAADASREDATTRPLSSHPPRVPVREDEPATLA
                     DVLERAVREHARPNLLNYKRDGAWRAISSEEFMARARRAALGLHSLGLGRGDRVAILS
                     ENCPEWTLTDAGAQLAGVVDVPIYSTQAPPQVCYILNDSGARVLFVQNRAAYDRVAAA
                     IGECASVEHVVFLLETEAQADIPGSLTYAEFEERASALERERPELVDELKAAVRPEEV
                     ATIIYTSGTTGEPKGVMLTHSNLVSNIVDVSELLDWEGGDIALSVLPLSHVFERMAMY
                     MYIHQGMSVYYAESIEKLGENIREVRPTVLVAVPRLFEKIYSRIKEKAEAGGKLKAGL
                     LAWAVETGKRWAALKLKGEEIPPLLSLKQEIASRLVFSKWREGLGGRIRMFVSGGAAL
                     PEEIGYIFAGAGIPIMQGYGLSETSPVIAANSLRDNRMGTVGHPIRNVEVRIAEDGEI
                     ETRGPNVMLGYYNKPEATRDVFTADGWFKTGDIGALDEEGRLRITDRKKELFKTSGGK
                     YVAPQPIEQRIKQSRFVNQVVLIGNGRKFPAALVVPDWEMLRSYAQLKNIDAKTPAEL
                     CRHPRVLDLMQRQIDAQCQELSKYERVKRVALLEKELTIDGGELTPTLKVKRRVIDDK
                     YRDVIDDLYAE"
     gene            complement(2582..3199)
                     /locus_tag="AC159P_16920"
     CDS             complement(2582..3199)
                     /locus_tag="AC159P_16920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR3697179.1"
                     /translation="MRSARTLPQQTRPAVADKREAILRAATRVFAESGYFNSKVADIA
                     RVAGVADGTVYLYFKSKEDILHSIFERNTSEAIREGREELRKIEDPREKLRRIARHHL
                     ERLGSDRDLAVVFQVELRGSTKFMEAFSAAGLAEYLHLIREVFEEGQRAGLFRADLNA
                     KLVAKILFGALDEMATNWILSKRRYKLAPMADEVLDIFLNGVSAR"
     gene            complement(3446..>4309)
                     /locus_tag="AC159P_16925"
     CDS             complement(3446..>4309)
                     /locus_tag="AC159P_16925"
                     /inference="COORDINATES: protein motif:HMM:NF044972.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HIR3697180.1"
                     /translation="TLALGLLVFCVAVLVSRYARAFLERRMTARRLDAGVQYNVLRLI
                     HYFVLVVGIISALRVAISADFTSIAVVFTALSVGIGFGLQFIAGDIASGFILLFERPV
                     RVGDYVSIPGPDGKITEGRVKSINLRAAIVATNDNIAAVVPNSKLVNQTFLNWSYREG
                     RSRNLTRVSVYVGVSYEANVEVVTETLLRAAEGVQFVLEEPKPSVQFLEFGESALNFR
                     LLVWTDRPRRHPAIKSDINYRIRRLFNEESIAIANPQRDIYLRGGSLSIDPRAPRPAE
                     EEDAEPANNKR"
BASE COUNT          677 a         1372 c         1450 g          810 t
ORIGIN      
        1 ggccacgtcg tgctcttcga gccgcacgag cggaaggtct tcgtcggcga ctgcctgttc
       61 gcgggttcga tcgggaggac ggacttgccg ggcggctcga cggagcaact gttggactcg
      121 atcaccaaca agattctccc gctcggcgac gacgtggtgg tctactccgg ccacggcccc
      181 gaaaccacca tcggccacga gcggctgacc aaccccttcc tcaccggcgg ctataagctc
      241 gggagatacc tgtaagagcc gtgaaccgtg aaccgtgata agaagaaagc ggtttttcac
      301 ttatcacggt tcacggcttt tggccttcct cggcgggctt gtggaggcgc tcgttcttga
      361 gcttctccca cttctctttg acgtcgccct ggaggtcgct cgggagcgtt tcgacaggga
      421 cgccgtgctc ggcgcagaag gtgcgccaga gcatgaagcg ggcgtcgaac tctcccgtcc
      481 cttcgttgat ttgattcgga actttgggtt tctctttaga cacgaatctc gcgctcctcg
      541 ctcccgcttg atgttcgacc gccacggccc gcgcacagtg tagcagaagc ggtggccggg
      601 gataaggatg ggtgttgggt gttgggtgtt ggggaagaca actgctgata gccaacaccc
      661 aacaccctgt cttgctgcta ttccgcgtag aggtcgtcga tcacgtcgcg gtacttgtcg
      721 tcgatgacgc ggcgcttgac cttgagcgtg ggcgtcagct cgccgccgtc gatggtcagc
      781 tccttttcga gcagcgcgac ccttttgacg cgctcgtatt tcgaaagctc ctggcactgc
      841 gcgtcgatct gccgctgcat gaggtcgagg acgcgcggat ggcggcagag ttcggcgggc
      901 gtcttggcgt cgatgttttt cagttgcgcg taggagcgga gcatctccca gtcggggacg
      961 acgagcgcgg cggggaactt cctgccgttg ccgatgagca cgacctgatt gacgaagcgc
     1021 gactgcttga tgcgctgctc gatgggttgc ggggcgacgt acttgccgcc cgaggtcttg
     1081 aacagctcct tcttgcggtc ggtgatgcgg aggcgccctt cttcgtcgag cgcgccgatg
     1141 tcgccggtct tgaaccagcc gtcggcggtg aacacgtcgc gcgtcgcctc gggcttgttg
     1201 tagtagccga gcatgacgtt ggggccgcgc gtctcgatct cgccgtcctc ggcgatgcgc
     1261 acctcgacgt tgcggatagg gtggccgacg gtgcccatgc ggttgtcgcg caggctgttg
     1321 gcggcgatga ccggcgaggt ctcgctcagc ccgtagccct gcatgatcgg gatgcccgcg
     1381 cccgcgaaga tgtagccgat ctcttcgggc agcgccgcgc cgcccgagac gaacatccgg
     1441 atgcgaccgc cgagtccttc gcgccacttc gagaagacga gccgcgacgc gatctcctgc
     1501 ttcaaagaga gcagcggcgg aatctcttcc cctttgagct tcagcgcggc ccagcgcttc
     1561 ccggtctcga cggcccacgc gagcaggccc gccttgagct tgccgcccgc ttcggccttc
     1621 tccttgatgc gcgagtaaat cttctcgaac agccgcggca cggcgacgag gacggtcggc
     1681 ctcacctcgc ggatgttctc gccgagcttc tcgatcgact cggcgtagta gacgctcatg
     1741 ccctgatgga tgtacatgta catcgccatc cgctcgaaga cgtgcgagag cggcaagacc
     1801 gagagcgcga tgtcgccgcc ctcccagtcg agcagctcgc tcacgtccac gatgttcgag
     1861 acgagattcg agtgcgtgag catcaccccc ttcggctcgc ccgtcgtgcc cgacgtgtag
     1921 atgatcgtcg ctacctcttc cggcctgacg gcggccttca gctcatcgac cagctcgggc
     1981 cgctcgcgtt cgagcgcgga ggcgcgctcc tcgaactcgg cgtaagtgag cgagccgggt
     2041 atatccgctt gcgcttccgt ttcgagcagg aagacgacgt gctcgaccga ggcgcattcc
     2101 cctatggccg cggcgacgcg gtcgtaggcc gcgcggttct ggacgaagag gacgcgcgcg
     2161 cccgagtcgt tcaggatgta gcagacctgc ggcggcgcct gcgtcgagta gatggggaca
     2221 tcgaccacgc ccgccagttg agcgcccgcg tccgtgagcg tccactccgg gcagttctcc
     2281 gaaaggatcg ccacgcggtc gccgcgcccg aggccgaggc tgtgcagccc gagcgccgcg
     2341 cggcgcgcgc gcgccatgaa ttcttccgaa gagatcgcgc gccacgcgcc gtcgcgcttg
     2401 tagttgagga ggttcgggcg cgcgtgctcg cgcacggccc gctccaacac gtcggcgagc
     2461 gtcgccggct cgtcctcgcg cacgggcacg cgcggcgggt gcgaggagag cgggcgcgtc
     2521 gtcgcgtctt cccgtgaggc gtcggcggcg gactgtattt cgcgggtcgt gttcgtcgtc
     2581 gtcatcgtgc gctcaccccg ttcaggaaaa tgtcgagcac ttcgtcggcc atcggcgcga
     2641 gcttatagcg ccgcttcgag agtatccagt tggtcgccat ctcgtccaag gcgccgaaca
     2701 gaatcttggc gacgagcttc gcgttgaggt ccgcgcggaa caaccccgcg cgctgcccct
     2761 cctcgaagac ctcgcggatg agatgcaggt actcggccag accggcggcg gagaatgcct
     2821 ccatgaactt ggtcgagccg cgcagctcga cctgaaagac gaccgccagg tcgcggtctg
     2881 agccgagccg ttcgaggtgg tggcgcgcga tccttcgcag cttctcgcga gggtcttcga
     2941 ttttgcgcag ctcctcgcgc ccctcacgaa tggcctcgct cgtgttgcgc tcgaagatgg
     3001 aatgcaggat gtcttccttg ctcttgaagt agagatagac ggtgccgtcg gccacccctg
     3061 cgacgcgcgc gatgtcggcg accttcgagt tgaagtagcc gctctcggcg aagacgcgcg
     3121 tagcggcgcg caggatggcc tcgcgcttgt cggcgactgc cgggcgcgtc tgctgcggga
     3181 gggtacgggc ggaacgcatc ggctctctct ggctccggac gttgacggac gttctgctga
     3241 atggctgttc attcagtcgc gtgcccccta gcctaacctc tcggcgcgcg tttgcgcaac
     3301 agaagcgggc tgtctctcga tttcggattc cggatttcgg attgcggatt gatgagggcg
     3361 ccgccgctca tgagcgaagc ctgacggcag cggcgctgtc atcaatcgtc aaccgtcaat
     3421 ccggaatccg gaatccggaa tcgtctcacc gcttgttgtt cgcaggctct gcgtcttcct
     3481 cctcggcggg gcgcggggcg cgcgggtcga tgctgagcga gccgccgcgc aggtagatgt
     3541 cgcgttgcgg gttcgcgatg gcgatgctct cttcgttgaa gagccggcgg atgcggtagt
     3601 tgatgtcgct cttgatggcg gggtggcggc gcggcctgtc cgtccagacg aggaggcgga
     3661 agttgagcgc cgactcgccg aactccagaa actgcaccga gggcttcggc tcttcgagga
     3721 cgaactgcac gccctctgcg gcgcgcagca gcgtctcggt cacgacctcc acgtttgcct
     3781 cgtacgagac gccgacgtag acggagacgc gcgtcaggtt gcgcgagcgc ccctcgcggt
     3841 acgaccagtt gaggaaggtc tggttgacga gcttcgagtt gggcacgacc gccgcgatgt
     3901 tgtcgttcgt cgcgacgatg gccgcgcgca ggttgatgct cttgacgcgc ccctcggtga
     3961 tcttgccgtc ggggccgggg atggacacgt agtcgccgac gcgcacaggc cgctcgaaca
     4021 gcaggatgaa gcccgaggcg atgtcgcccg cgatgaactg caggccgaag ccgatgccga
     4081 ccgagagcgc cgtgaagacg accgcgatgg acgtgaagtc ggcggagatg gcgacgcgca
     4141 gcgccgagat gatgccgacc accagcacga agtagtggat gagccgcagc acattgtact
     4201 ggacgcccgc gtcgagccgc cgcgccgtca tccgccgctc cagaaaggcg cgcgcgtagc
     4261 gcgagacgag caccgccacg cagaacacca agaggccgag cgccagtgt
//
LOCUS       DBNGOH010000875         3788 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2331146, whole genome shotgun sequence.
ACCESSION   DBNGOH010000875 DBNGOH010000000
VERSION     DBNGOH010000875.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3788)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3788)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3788
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2331146"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..469)
                     /locus_tag="AC159P_16930"
     CDS             complement(<1..469)
                     /locus_tag="AC159P_16930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697181.1"
                     /translation="MFVTAVENFVLFGTLLALGGTAFAWAASTAALRGWWWPHPLTLT
                     RVYSLALLIPPVTATWIVAAAFLPEWWLGEEAFGAAHPAPLHQLHLLSDLTAALEPRL
                     AYATLAFAAAASAFAAVSSARGYARVGLVIRRLEMGIAPPRPEQVEVVRAAVAR"
     gene            complement(469..912)
                     /locus_tag="AC159P_16935"
     CDS             complement(469..912)
                     /locus_tag="AC159P_16935"
                     /inference="COORDINATES: protein motif:HMM:NF015898.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HIR3697182.1"
                     /translation="MRDNPKITLRGFRPGSRGAGLVLGELQTAVMEILWRESGLTVNE
                     VEERLRKKRALAHTTVLTTLDRMHASGFLLREKQGKAFVYSPRYTREEFERGMAQEVL
                     GALLGQLTEPAISAFVDLVGEDDGALDRLEEVIRKKRREKGQRRK"
     gene            complement(1094..1753)
                     /locus_tag="AC159P_16940"
     CDS             complement(1094..1753)
                     /locus_tag="AC159P_16940"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3697183.1"
                     /translation="MEVTRRFGGRAAVDRVSLDVGEGEVVALLGASGCGKTTLLRLIA
                     GLEAPDEGEVWVAGRRAAAEGRNLVPPHARSVGFVFQDLALWPHLTVAGNLDFVLGAA
                     RLKKRDRRARIDEALRLVRAEAFAHKFPGQLSGGEQQRAAIARAIVARPQLLLLDEPM
                     SSLDADLKAQLRDEFISLQRRIGVTTVYVTHDLAESAAVAHRTVLMRGGRMISDPELS
                     G"
     gene            complement(1777..3420)
                     /locus_tag="AC159P_16945"
     CDS             complement(1777..3420)
                     /locus_tag="AC159P_16945"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3697184.1"
                     /translation="MGSDTYYTKAVGRVRGAMSARAAILAVAVALFVALCVLPIIYMF
                     GISLTGAGGDFSFDNYRRLMTEPRQRELLMNSTLLGAGVALLATALGTPTGLLLARTP
                     LPAKRLLRVVLVVPLVVPPYVLALAWILLSGPAGLLARAAGRDLLSGWTYSLPGTIVV
                     LGLGFYPLSMLATEAAARRVDGRLEEAALVVARPRRVLWRITLPLIAPAVAATALIIF
                     VLALSEFGVPGLLRVRVFTTEVFTAFAALYDFGAGVALATPLLAAALLAGVVVKLIMG
                     ERLLAARRGEHPGLPMPLRRWRVPVLIALASMVFTSILLPLVALAVEAGGVRRIASAV
                     RTSGEAIANSLWLAAVGATLIFALAVVLGYGRARTRTRFRGLIDLAFIVIFAVPSTAV
                     GIGIIGLWNRPGLPGDVYTSSAIIVVAYLARFVPVAALILAAGVRQIPASFEEAAEVA
                     GAGWPRTFTRIVLPNMRASVAAAWVVAFIFAFGELGATILVAPPGESTLPFRVYTLIA
                     NTPPSEVAALALVQVGILLAPLSLLGFFARGDAGRGGAR"
     gene            complement(3434..>3788)
                     /locus_tag="AC159P_16950"
     CDS             complement(3434..>3788)
                     /locus_tag="AC159P_16950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013820727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HIR3697185.1"
                     /translation="LTDTDDVNVALEDKQPVAMVLPDRDGMGAALMPNMVSLIARAPH
                     ADAGRKLIDYLLSAEVETALAQSEAVQIPLREGVEGPKNIPPLSSFKPMTLDYGRAAD
                     RVEEVTRRLQPILGL"
BASE COUNT          693 a         1288 c         1294 g          513 t
ORIGIN      
        1 gacgcgccac ggccgcgcgg acgacttcga cctgttcggg ccggggcggg gcgatcccca
       61 tctccagtcg cctgatgacg agcccgaccc gcgcgtagcc ccgcgcgctc gacacggccg
      121 cgaaggccga ggcggcggcc gcgaacgcga gcgtcgcgta tgcgagcctg ggttcgagcg
      181 cggccgtcag gtcgctgagc aggtggagct ggtgcagggg cgcggggtgc gcggccccga
      241 aagcctcctc gccgagccac cactctggca ggaacgccgc cgcgacgatc caggtcgcag
      301 taaccggggg gatgagaagc gcaagggagt agacgcgcgt gagcgtcagc gggtgcggcc
      361 accaccagcc gcgcagcgcg gccgtgctcg ccgcccacgc gaaggccgtg ccgccaagcg
      421 cgagcagcgt cccgaacaga acgaaatttt ctaccgccgt gacgaacatt acttgcgccg
      481 ctggcccttc tcgcgccgct tctttctgat cacctcttcg aggcgatcga gcgccccgtc
      541 gtcctcgccc accaggtcca cgaaggccga gatggccggt tccgtgagtt gcccgagcag
      601 cgcgcccaga acctcctgcg ccatgccgcg ctcgaactcc tcgcgcgtgt agcgaggcga
      661 gtagacgaaa gccttcccct gtttctcacg cagcaggaag ccgctcgcgt gcatccggtc
      721 gagcgtcgtg agcacggttg tgtgggcgag cgcgcgcttc ttacgtagac gctcctcgac
      781 ctcgttgacg gtgagccccg actcacgcca gagaatctcc atcacggcag tctgcaactc
      841 gccgaggacg agcccggcgc cgcgggagcc cggcctgaag ccgcgcagtg tgatcttcgg
      901 gttgtcgcgc atggttcgta aattctccgc aggtgttccg gggagaattc gcaaagtata
      961 gggaaggggc tgggaaaatt caactacacc cggtagtcgt aaagtaaccc tcaacaacat
     1021 acgccgcccg ctggctccgc atagcgttac gcgaaaatct aagactcttg ccaagttcct
     1081 tcgtcgagaa ctcctaacct gaaagctccg ggtcggagat catgcgcccc ccgcgcatca
     1141 atactgtgcg atgggcgacc gccgcagact ccgctaggtc gtgcgtgacg tagacggtcg
     1201 tcacaccgat ccggcgttgc aaagaaatga attcgtcgcg caactgcgct ttcaggtcgg
     1261 catcgaggct cgacatcggc tcgtcgagaa ggagcagctg cgggcgggcg acgatggcgc
     1321 gcgcgatggc ggcgcgttgc tgttccccgc cggagagttg gcccggaaac ttgtgggcga
     1381 aggcttcggc gcggacgagc cgcagcgcct cgtcaatccg cgcgcgccgg tctcgcttct
     1441 tgagccgggc ggccccgaga acgaaatcga ggttgcctgc gaccgtcagg tgcggccaca
     1501 gggcgaggtc ctgaaaaacg aaacccacgc ttcgcgcgtg aggcggcacg agattgcggc
     1561 cttcggccgc cgcgcgacgc cccgcgaccc agacctcccc ttcgtccggc gcctccagcc
     1621 cggcgataag gcgcaagagg gtcgtctttc cgcacccgct cgcgccgagt agcgccacga
     1681 cttcaccttc acccacgtcg agcgagacgc ggtccacggc cgcacgaccg ccgaatcgcc
     1741 tggtcacttc cacgagtcgg aggaaagggt cgccagtcac cgcgcccctc cccggcccgc
     1801 atcgccgcgg gcgaagaagc ccaacaggga gagcggggcg agaagaatac ccacttgcac
     1861 gagcgccagc gccgccacct cggagggggg cgtgttggcg atgagtgtgt agacgcgaaa
     1921 cggcagggtg gactcgcccg ggggggcgac gaggattgtg gcgccgagtt cgccgaaggc
     1981 gaagatgaaa gccacgaccc aggcggcggc gacgctcgcg cgcatgttcg ggaggacgat
     2041 gcgtgtgaac gtgcgcggcc agcccgcgcc ggcgacctcg gcggcctcct cgaacgaggc
     2101 ggggatctgg cgcacgcccg ccgcgaggat gagcgcggcg acggggacga agcgcgcgag
     2161 ataggccacg acaatgatcg cggacgacgt ataaacgtcc cccggcaatc ccggtctgtt
     2221 ccacagcccg atgatcccga tgccgaccgc ggtgctcggc acggcgaaga tgacgatgaa
     2281 ggcaagatcg atgaggccgc gaaagcgagt ccgcgtccgc gcccgcccgt aaccgagcac
     2341 taccgccaaa gcgaagatca acgtcgcgcc gacggcggcg agccagaggc tgttggcgat
     2401 ggcctcgccg gatgttcgca cggcggacgc gatgcgcctc acccctcccg cctccacggc
     2461 cagggcgacc agcggcaaaa gaatcgaggt gaaaaccatg gaagcgagtg cgatcagtac
     2521 gggcacgcgc cagcgccgga ggggcatagg caaacccgga tgctcgccgc gccgcgccgc
     2581 gagcagtcgc tcgcccatga tgagtttgac tacgacgccg gcgaggagcg ccgccgcgag
     2641 cagcggcgtg gcgagcgcca cgcccgcgcc gaagtcgtag agcgcggcga aggcggtgaa
     2701 gacctccgtg gtaaagacgc gcacgcgcag gaggcccggc acgccgaact ccgagagcgc
     2761 gagcacaaag atgatgagcg cggtcgccgc caccgccggc gcgatgagcg gcagagtgat
     2821 ccgccacaac acgcgacgcg ggcgggcgac gacgagcgca gcctcttcga gccgcccgtc
     2881 cacgcgccga gccgccgcct cggtcgccag catcgagagc gggtagaagc cgaggccgag
     2941 cacgacgatg gtgcccggca gactgtacgt ccaccctgaa aggaggtcgc ggcccgcggc
     3001 ccgcgcgagg agccccgccg gaccgctcaa cagaatccac gcgagggcca gcacgtaagg
     3061 cgggacgacg agcgggacga cgagcacgac gcgcaatagt cgtttcgccg gcaggggtgt
     3121 gcgggccagc agcagccccg tcggcgtccc cagcgcggtc gcgagcagcg cgacgcccgc
     3181 gcccagcagg gtgctgttca tcaacagttc ccgctggcgc ggctccgtca tcagacggcg
     3241 ataattgtcg aagctgaagt cgccgcccgc gcctgtgagc gagatgccga acatataaat
     3301 gatgggtagc acgcacagcg cgacgaacag ggcgacggcc acggcgagta tggcggcgcg
     3361 cgctgacatt gcaccgcgga cgcgcccgac cgccttcgtg taataggtgt cacttcccat
     3421 cactccgacg cgctcagagt ccgaggatgg gttgcagacg gcgcgtcact tcttcgacgc
     3481 ggtcggcggc cctaccgtag tcgagcgtca tcggcttgaa ggaagaaagc ggcgggatgt
     3541 tcttcggccc ctcgacgcct tcgcgcagcg ggatctgcac ggcctccgat tgcgccagcg
     3601 ccgtctcgac ttccgccgaa agcaggtagt cgatcagctt cctgcccgca tcggcgtgcg
     3661 gcgccctcgc gatcagcgaa accatgttcg gcatcaacgc cgcgcccatc ccgtcacggt
     3721 cgggcaggac cattgccacg ggctgtttgt cttcgagcgc gacgttgacg tcgtcggtgt
     3781 cggtcagc
//
LOCUS       DBNGOH010000876         8151 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3797739, whole genome shotgun sequence.
ACCESSION   DBNGOH010000876 DBNGOH010000000
VERSION     DBNGOH010000876.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8151)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8151)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8151
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3797739"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2329
                     /locus_tag="AC159P_16955"
     CDS             <1..2329
                     /locus_tag="AC159P_16955"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013939645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000428 - DNA-directed RNA polymerase
                     complex [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HIR3697186.1"
                     /translation="TKAGISIGIDDLVTPDAKKTLVAQADKEVIQIEKQYRDGVITNG
                     ERYNKVIAIWSEVTDKVAKEMFRAMDQKEKEAGEQNPILIMSDSGARGTAQQIRQLAG
                     MRGLMAKPSGEIIETPIRSNFREGLNVLQYFISTHGARKGLADTALKTADSGYLTRRL
                     VDVAQDVIISEPDCGTLKGISSTAIVEGGEEIESLRDRIVGRTALEDVIDPVEGRVIV
                     ESNGEITEDLAQEIQRSGVQRVRIRSVLTCESRRGVCIKCYGRNLATGRPVDIGEAVG
                     VIAAQSIGEPGTQLTMRTFHIGGTARLEESSKHEARMDGTIKYVELNVVKNRRGEIIA
                     MNRNGALTIIDERGREVARHQIVYGAHIHVENGSRVKEDQLLATWDPFTFAILTEVAG
                     QVKYQDLKEGVTVEEEVDEVTGQSRVVVKDSPDEKRQPRLEIRAGTKVLKTYQMPVRA
                     NLMVHDGDTVEPGDIIAKIPRETTKTKDITGGLPRVVELFEARKPRETAIMSEIDGAV
                     TLGTISKGKRKLIITGDDGEQREYDIPRGTHINVQDGDRVRAGEALMDGPLNPHDILR
                     VLGMNRLQEYLTNEIQEVYRLQGVNINDKHIEVIVRQMLRWVKVTDVGDTDFLLDEQV
                     DRFRFSDENDRVRAADGEAANADNLLLGITKASLSTDSFISAASFQETTRVLTEAAIS
                     GRVDYLRGLKENVIMGRLIPAGTGMEFYRNVKVDRDPTVDQAREEQAIESLSDLLGGE
                     EPTLPAPRERPVPAPVIADGDGDADEADTDEGDDE"
     gene            complement(2594..3292)
                     /locus_tag="AC159P_16960"
                     /pseudo
     CDS             complement(2594..3292)
                     /locus_tag="AC159P_16960"
                     /inference="COORDINATES: protein motif:HMM:NF013672.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
     gene            complement(3403..4077)
                     /locus_tag="AC159P_16965"
     CDS             complement(3403..4077)
                     /locus_tag="AC159P_16965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697187.1"
                     /translation="MAVQQGRQNVLDSYVTVAERIAAFYQSYPTGRICTQILEHHEES
                     GFILMRAEVFRSTDDAQPSATGHAFEVRGDGYVNRTSYIENAETSAVGRALALLGFEV
                     RRGIASREEMEKAARMPAERAARAPESKPPESKPPEGKPAAKPAPPPAAAESPSAEES
                     PNLDDDIIRNAQQLGYDAAKVRRWVNQKFGVTGGLESLTEHDKREVLKLFREQAQAAQ
                     AKSPRK"
     gene            4714..5121
                     /gene="rpsL"
                     /locus_tag="AC159P_16970"
     CDS             4714..5121
                     /gene="rpsL"
                     /locus_tag="AC159P_16970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012626196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HIR3697188.1"
                     /translation="MPTINQLVRKGRQAVKYKTASPALQSSPQKRGVCTRVYTQTPKK
                     PNSALRKVARVRLTNSIEVTTYIPGVGHNLQEHSIVLIRGGRVKDLPGVRYHVIRGTL
                     DSVGVADRKQGRSKYGAKRPKAGAAAGAAKGKR"
     gene            5251..5721
                     /gene="rpsG"
                     /locus_tag="AC159P_16975"
     CDS             5251..5721
                     /gene="rpsG"
                     /locus_tag="AC159P_16975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012525971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HIR3697189.1"
                     /translation="MPRRRVVDRREVLPDPVYNSPLVTKFISGIMWDGKKSVAEGIFY
                     RALQQMGEKTGEDPLKLFKKAIDNVRPTVEVKSRRVGGSTYQVPIEVPQERRGSLAIR
                     WITTAARARGEKTMIDRLAGEMLDAANNRGNAVKKKEDTHRMAEANKAFAHYKW"
     gene            5811..7913
                     /gene="fusA"
                     /locus_tag="AC159P_16980"
     CDS             5811..7913
                     /gene="fusA"
                     /locus_tag="AC159P_16980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HIR3697190.1"
                     /translation="MAKQDLSKFRNIGIMAHIDAGKTTTTERVLYYTGVSHKIGEVHE
                     GTATMDWMEQEQERGITITSAATTCFWTRSGVEHRINIIDTPGHVDFTMEVERSLRVL
                     DGAVAVFDGVAGVEPQSETVWRQADKYGVPRICFINKLDRAGASFERSFQSIIDRLGA
                     NPVALQIPIGHEDQFKGVVDLITMKGLLWKDETKGAEYETGEVPAELKAEAEAMRHKL
                     LEAVAEADEDLMEQYLEDEGASFTEEQIRAALRKGTIALKIVPVVTGSAFKNKGVQTL
                     LDAVVDYLPSPLDIPPIEGINPRTGETELRPPDAAAPFSGLVFKIMADKHLGQLSFVR
                     IYSGTTRAGSYAYNSVKDTKERVGRLMRMHANKREDIDEASAGEIIAIGGMKQVTTGD
                     TVCDENKPIILEAMEFPAPVIRVAVEPKTRADQDKLGTALSRLAQEDPSFNVSTDQET
                     GQTIIAGMGELHLEIIVDRMKREFGVEANVGKPQVAYRETITQAAPGKEVYKRQTGGR
                     GQYGHVEIEIEPAPGEGFVFENKITGGVIPREFIKPSEEGIRDAMERGFLAGYELVDV
                     RVRLVFGSYHEVDSDERSFHIAGSLAFQDAVKKAKPVLLEPIMKVEVVTPKDFVGAVT
                     GDLNRRRGRIEKMEPRPGGAEVVTAFVPLSEMFGYTTDLRSATQGRATSSMHFERYEQ
                     APKNIADEVIAKVQGVAK"
     gene            8013..>8151
                     /locus_tag="AC159P_16985"
     CDS             8013..>8151
                     /locus_tag="AC159P_16985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020715234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HIR3697191.1"
                     /translation="MSKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITKVLARHNPKIA
                     FR"
BASE COUNT         1577 a         2584 c         2716 g         1274 t
ORIGIN      
        1 gacgaaggcc ggcatctcca tcggcatcga cgacctcgtc acgcccgacg ccaagaagac
       61 gctcgtcgcg caggccgaca aggaggtcat ccagatcgag aagcagtacc gcgacggcgt
      121 catcacgaac ggcgagcgct acaacaaggt catcgccatc tggtcggaag tcaccgacaa
      181 ggtggcgaaa gagatgttcc gcgcgatgga tcagaaggag aaggaggcgg gcgagcagaa
      241 cccgatcctc atcatgtccg attcgggcgc gcgcggcacc gcgcagcaga ttcgccagct
      301 cgcgggcatg cgcggcctga tggccaagcc ctcgggcgag atcatcgaga cgcccatccg
      361 ctcgaacttc cgcgaggggc tgaacgtctt gcagtacttc atctcgacgc acggcgcgcg
      421 taaaggcttg gccgacacgg cgctcaagac cgccgactcc ggctacctga cgcgccgtct
      481 ggttgacgtg gcgcaggatg tcatcatctc ggagccggac tgcggcacgc tgaagggcat
      541 ctcttcgacg gccatcgtcg agggcggcga ggagatcgaa tctctgcgcg accgcatcgt
      601 cgggcgcacc gcgctcgaag acgtgatcga ccccgtcgag ggccgcgtca tcgtcgagtc
      661 gaacggcgag atcaccgaag acctcgcgca ggagattcag cgctccggcg tccagcgcgt
      721 ccgcatccgc tcggtgctga cctgcgagtc gcgccgcggc gtctgcatca agtgctacgg
      781 tcgtaacctc gctacgggtc ggcccgtgga catcggcgag gcggtcggcg tcatcgccgc
      841 gcagagcatc ggcgagccgg gcacgcagct cacgatgcgc accttccaca tcggcggcac
      901 cgcgcgcctc gaagagtcct cgaagcacga ggcgcgcatg gacggcacga tcaagtacgt
      961 cgagctgaac gtcgtcaaga accgccgcgg cgagatcatc gccatgaacc gcaacggcgc
     1021 gctgaccatc atcgacgagc ggggccgcga ggtcgcgcgc caccagatcg tctacggcgc
     1081 gcacatccac gtcgagaacg gctcgcgcgt caaggaagac cagctccttg ccacctggga
     1141 cccgttcacc ttcgccatcc tgacggaggt cgccgggcag gtgaagtatc aggacttgaa
     1201 ggagggcgtg acggtcgagg aggaggttga cgaggtgacg ggccagtcgc gcgtcgtcgt
     1261 caaggactcg cccgacgaga agcgccagcc gcgtctcgaa atccgcgcgg gcacgaaggt
     1321 cttgaagacc taccagatgc ctgtgcgcgc gaacctgatg gtgcacgacg gcgacacggt
     1381 cgagccgggc gacatcatcg ccaagattcc gcgcgagacg accaagacga aggacatcac
     1441 gggcggtctg ccgcgcgtgg tcgagctgtt cgaggcgcgc aagccgcgcg agacggccat
     1501 catgtccgag atcgacggcg ccgtcacgct cggcaccatc tcgaagggca agcgcaagct
     1561 gatcatcacg ggcgacgacg gcgagcagcg cgagtacgac atcccgcgcg gcacgcacat
     1621 caacgtgcag gacggcgacc gcgtccgcgc gggcgaggcg ctgatggacg gcccgctgaa
     1681 cccgcacgac atcctgcgcg tgctcggcat gaaccgcttg caggagtacc tgacgaacga
     1741 aatccaggag gtctaccgcc tccagggcgt caacatcaac gacaagcaca tcgaggtcat
     1801 cgtccgccag atgctgcgct gggtgaaggt gacggacgtg ggcgacacgg acttcctgct
     1861 cgacgagcag gttgaccgct tccgcttctc cgacgagaac gaccgcgtcc gcgccgccga
     1921 cggcgaggcg gccaacgccg acaacctcct gctcggcatc acgaaggcgt cgctctcgac
     1981 cgactccttc atctccgccg cctccttcca ggagacgacg cgcgtcctga cggaggccgc
     2041 catctcgggc cgcgtcgatt atctgcgcgg cctcaaggag aacgtcatca tgggccgcct
     2101 gattccggcg ggcaccggca tggagttcta ccgcaacgtc aaggttgacc gcgacccgac
     2161 cgtcgatcag gcgcgcgaag agcaggccat cgaaagcctc tccgacctgc tcggcggcga
     2221 ggagccgacc ctgcccgcgc cccgcgaacg ccccgtcccc gcccccgtca tcgccgacgg
     2281 tgacggcgac gcagacgaag cggacaccga cgagggcgac gacgagtaaa cccgactgcc
     2341 ttcacccgaa ggcgccggcg aatctcggca tcagcctcgg aacgaaatga ccggcgcagg
     2401 cttcgattag cctgcgccgg ttttttttat gagtgatacg aaggcgaggg agcgcacagc
     2461 tcaagctcgc cgacgcggat acgtgtggaa gtcgcgcgag gtggccgtca taactctcga
     2521 agcaaggacg cgttcgtccg agggcggggg ctggaggaag gcgaagtctc tggttcaacc
     2581 cgcccagacc gattcatgcc acgtgtcctt tgcgattccg aaaaatgtag tcggcgacct
     2641 ctttgacggg ggcgacccag accgacgcgc ctgtgaggta ggtgaggaac tggcggtgga
     2701 cgtggcggct gagctggttg ccgacggggg cgtcggtgtc gaggacatcg tggaacatga
     2761 agatggccca gtggccgcta cgggcggcgc cttcgcagta ggcccagagg tctctgaggc
     2821 ggactccgtc gggggcgtgg aagccggagg cgctgacgac ggtcagcggg cggcgtaggg
     2881 cgagcgggtc gttaggcgtg ccgaacgccg agcgtgcgac tttatggtat ctggagatgg
     2941 cgttcgcgta agagtcgctg tcgctgttcc cggtgccgat gccgagctgg ccatgcgggt
     3001 aggcgaagct gcggtcgggg tcgtggccga tgctcctttt gagccacagc gcgccgtgcc
     3061 cgacctcggc caggatgtcg cccgcgtcgt agtcggacag ctcgtccttc gaagggtggt
     3121 tgtaggagtg gttgccgatt tcgtggcccc ttgagaaggc ggcccgccag cggtcgaggc
     3181 agcagtccac ttcggagttg ttcgtcaggt agaaggtgcc gcggaagcgg aactcgtcga
     3241 ggtcggggat ggccacgtcg aggtggttga gcctgccgtc gtcgtaggtg aggctgaccg
     3301 ccgccctcgc ccgatgaggc cagcgccagt ggtcgaaggg acgtgcggcg caactggtcg
     3361 tgaggttgac tccttatcag gttttattcc gcggcgccgt cgtcatttgc gcggggactt
     3421 ggcctgcgcg gcctgcgcct gctcgcggaa aagcttcagg acttcgcgct tgtcgtgctc
     3481 ggtgaggctt tcgagtccgc ccgtgacgcc gaacttctgg ttgacccagc ggcgcacctt
     3541 ggccgcgtcg tagccgagct gctgcgcgtt gcggatgatg tcgtcgtcga gattcggact
     3601 ctcctcggcg gacgggcttt cagccgcggc gggcgggggg gcgggcttcg ccgcgggctt
     3661 accctccggc ggcttgctct ccggcggctt gctctccggc gctcgcgcgg ccctctcggc
     3721 gggcatgcgc gcggccttct ccatctcctc gcggctggcg ataccgcgcc tgacctcgaa
     3781 gccgagcagc gcgagcgcgc gaccgacggc ggaagtctcg gcgttctcaa tataggaagt
     3841 tcgattgacg tagccgtcgc cgcggacttc gaaggcgtgg ccggtggccg agggctgcgc
     3901 gtcgtcggtc gagcggaaga cctccgcgcg catgaggatg aagccgctct cctcgtggtg
     3961 ttcgagaatc tgcgtgcaga ttctccccgt cgggtacgac tggtaaaagg ccgcgatccg
     4021 ttcggccacg gtgacgtaag aatcgagcac gttctgcctg ccctgctgta cggccatctc
     4081 gggtttcctc ttcgtcgaat cggcgggcaa ttccggcccg tctcgcgcat cgaagtagtt
     4141 cgcgtaaagc ttacgggacg cactctagca gaaagccgga ggcggcggaa cagggaaaat
     4201 tccggacgga agcggaacgc ctccgtgcca acttgacagg gagcggctgc gcccgtatag
     4261 tctcacgttt ggactgctag tccatttccc aagagtgcaa cgcttgattg gtttcggccg
     4321 gtgtggaggc gttgcccggc tatgtcccga gagggaaata gtcgcttgac aattattgtg
     4381 gcgtaaactt cggcgccccg ccttcggcgc ccctacgggg gccgcgggcg gccaagcgac
     4441 cgcggcgcga aaccagaggg ggttagaggt tcgaccctcc ttcccgaaca ggcgacgcca
     4501 cagaaaagtt tcggctctgg gggagccaga atccacaccc gtctcaagta gagcgaagtc
     4561 ggaacatccc cgaagtttaa tcaatagtga agggcacgcc gctcttgcgc tgactgccgg
     4621 actccgctga gagagttccg gtaagaggca gggaatggca gccgattgcg ccttgtaagg
     4681 cattaaccga acccgaagag gacggaaaca gcagtgccga ccatcaacca gttagtccgc
     4741 aagggccgcc aggcggtgaa gtacaagacg gccagcccgg cgctccaatc cagcccgcag
     4801 aagcgcggcg tctgcacgcg cgtctacacg cagacgccca agaagccgaa ctcggctctg
     4861 cgcaaagtgg cccgcgtccg cctcacgaac tccatcgagg tgacgaccta catccccggc
     4921 gtcggccaca acttgcagga gcactccatc gtgctcatcc ggggcgggcg cgtgaaggat
     4981 ttgccgggcg tgcgctacca cgtcatccgc ggcacgctcg actcggtcgg cgtcgccgac
     5041 cgcaagcagg ggcgctcgaa gtacggcgcc aagcgcccga aggccggcgc cgccgcgggg
     5101 gccgcgaagg gcaagaggta gggaagctgt tggccgttag ctcttagctt ttagcttagg
     5161 ctctacggcg agcggctcgg attctaacga agacggttag cgcggttagg tttttgctaa
     5221 cagctaatcg ctaatagcta agagctgact atgccaagaa gaagagtagt tgacaggcgc
     5281 gaggtgctgc ccgacccggt gtacaacagc ccgctggtga cgaagttcat cagcggcatc
     5341 atgtgggacg ggaagaagtc tgtggccgag gggattttct accgggcgct tcagcagatg
     5401 ggcgagaaga cgggcgaaga ccccttgaag ctgttcaaga aggcgatcga caacgtgcgc
     5461 ccgaccgtcg aggtcaagtc gcgccgcgtc ggcggctcga cctaccaggt gcccatcgag
     5521 gtgccgcagg agcgccgcgg ttctctggcg atccgctgga tcaccaccgc cgcccgcgcc
     5581 cgcggcgaga agacgatgat cgaccgcctc gccggcgaga tgctcgacgc cgccaacaac
     5641 cgcggcaacg ccgtcaagaa gaaagaggac acgcaccgca tggcggaggc caacaaggct
     5701 ttcgcgcact ataaatggta gcaagcaggt aaatagtaaa tggtcaatgg tgaatagaag
     5761 agagctttcc accattcact atttaccatt caccatttac gggtttcgtc atggctaagc
     5821 aagacctcag caagttccgc aacatcggca tcatggcgca catcgatgcc gggaagacga
     5881 cgaccacgga gcgcgtcctc tactacacgg gcgtctcgca caagatcggc gaggtgcacg
     5941 aggggacggc gacgatggac tggatggagc aggaacagga gcgcggcatc accatcacca
     6001 gcgccgcgac gacctgcttc tggactcgct ccggggtcga gcaccgcatc aacatcatcg
     6061 acacgccggg ccacgtggac ttcacgatgg aggtcgagcg tagccttcgc gtgctcgacg
     6121 gcgcggtcgc cgtgttcgac ggcgtcgcgg gcgtcgagcc gcagtcggag accgtctggc
     6181 ggcaggccga caagtacggc gtgccccgca tctgcttcat caacaagctc gaccgcgcgg
     6241 gcgcctcctt cgaacgctcc ttccagtcga tcatcgaccg cctcggcgcc aaccccgtcg
     6301 ccctccagat ccccatcggc cacgaagacc agttcaaggg cgtcgtggat ttaatcacga
     6361 tgaaggggct gctgtggaag gacgagacga agggcgccga gtacgagacg ggcgaggttc
     6421 cggcggagct gaaggccgag gccgaggcca tgcgccacaa gcttttggaa gcggtcgccg
     6481 aggctgacga agatttgatg gagcagtacc tcgaagacga gggcgcttcg ttcaccgagg
     6541 agcagattcg cgcggcgctc cgcaagggca ccatcgcttt gaagatcgtc ccggtcgtca
     6601 ccggctcggc cttcaagaac aagggcgtgc agactttgct cgacgcggtc gtggactatc
     6661 ttccttcgcc gctcgacatc ccgccgatcg agggcatcaa cccgaggacg ggcgagaccg
     6721 aactgcgccc gcccgacgcc gcggcgccgt tctccggcct cgtcttcaag atcatggccg
     6781 acaaacacct cggccagctc tcgttcgtcc gcatctactc gggcacgacc cgcgccggct
     6841 cctacgccta caactcggtg aaggacacga aggagcgcgt cgggcgcctg atgcgaatgc
     6901 acgccaacaa gcgcgaggac atcgacgagg cgagcgccgg cgagatcatc gccatcggcg
     6961 gcatgaagca ggtcacgacc ggcgacaccg tgtgcgacga gaacaagccc atcatcctcg
     7021 aagcgatgga gttcccggcg cccgtcatcc gcgtcgccgt cgagccgaag acgcgcgccg
     7081 accaggacaa gctcggcacg gccctgtcgc gcctcgcgca ggaagacccg agcttcaacg
     7141 tctcgaccga tcaggagacc ggccagacca tcatcgccgg catgggcgag ctgcacctcg
     7201 aaatcatcgt cgatcgcatg aagcgcgagt tcggcgtcga ggccaacgtc ggcaagccgc
     7261 aggtcgcgta ccgcgagacg atcacgcagg cggcgcccgg caaggaagtc tacaagcgcc
     7321 agaccggcgg tcgcggccag tacggccacg tcgagatcga gatcgagccg gcgcccggcg
     7381 agggcttcgt cttcgagaac aagatcacgg gcggcgtcat cccgcgcgag ttcatcaagc
     7441 cctcggagga gggcatccgc gacgcgatgg agcgcggctt cctcgccggc tacgagctgg
     7501 tcgatgtgcg cgtccgtctg gttttcggct cctaccacga ggtcgattcg gacgagcgct
     7561 cgttccacat cgcaggctcc ttggcgttcc aggacgcggt gaagaaggcc aagccggtct
     7621 tgctcgaacc gatcatgaag gtcgaggtcg tgacgccgaa ggacttcgtc ggcgccgtga
     7681 cgggcgacct caaccgtcgg cgcgggcgca tcgagaagat ggagccgcgg cccggcgggg
     7741 ccgaggtcgt gacggccttc gtgccgctct ccgagatgtt cggctacacg accgacctcc
     7801 ggtcggcgac gcaagggcgg gccacctcct cgatgcactt cgagcgctac gagcaggcgc
     7861 cgaagaacat cgccgacgag gtgatcgcca aggtgcaggg agtcgctaag taagtatcaa
     7921 gggcaggggt cgcttctctc gctgcattga caggcgtggc ggccccctaa aaaacggact
     7981 gacaaaaact ttttaggagc gagagaaaac agatgagcaa ggagaaattc gaccggtcga
     8041 agccgcacgt gaacatcggc accatcgggc acgtcgatca cgggaagacg accctcacgg
     8101 cggccatcac gaaagttttg gcgcgccaca acccgaagat cgcctttcgc a
//
LOCUS       DBNGOH010000877         2396 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4714737, whole genome shotgun sequence.
ACCESSION   DBNGOH010000877 DBNGOH010000000
VERSION     DBNGOH010000877.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2396)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2396)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2396
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4714737"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1813
                     /gene="topA"
                     /locus_tag="AC159P_16990"
     CDS             <1..1813
                     /gene="topA"
                     /locus_tag="AC159P_16990"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HIR3697192.1"
                     /translation="EIVAEAERQQFVVRDVTTKERKRNPVPPFITSKLQQEAARKLGF
                     AVKKTMMLAQKLYEGVELGAEGSVGLITYMRTDSTRVSETALAEARDFINAQYGPPYL
                     PEKAIHYRSKKDAQDAHEAIRPSDVARTPDSLARYLGKDELRLYRLIWQRFVASQMMP
                     AVFDQTTIDIEAGRFLFRATGSVLKFDGFLKVYEEGRDEKTDEDEEAARTLPLVEKGE
                     QLRLNAVTPEQHFTEPPPRYTEATLVKALEEKGIGRPSTYAAIMTTIQDREYVERVEG
                     RFHPTALGFTVNDLLVASFDDLFNESYTARMEEELDEVEEGKLKWTEALHEFYGKFAK
                     DLKTAETEMRKKKVEAIPTDEVCENCGSPMVIKFGRFGQFLACSNYPECRTTREVAKP
                     QGATAAAEDGAAKPAGVAGAEGDGVEETCDLCGKPMALKRGRFGQFLGCTGYPDCRNI
                     RKISKAGKVAPAPVPLEGETCPVDGAQLVRRHGPYGEFVSCANYPTCKYIKRETTGVA
                     CAKPGCKGEIVVKKSKRGKIFYGCSEYPKCDAVYWDKPVAEPCPQCNAPFLLEKTTKK
                     GTARYCARAEECGYREEALPHAPAPKSRSEGDTRFAR"
     gene            2017..>2396
                     /locus_tag="AC159P_16995"
     CDS             2017..>2396
                     /locus_tag="AC159P_16995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M28"
                     /protein_id="HIR3697193.1"
                     /translation="MPKRRMTALLLFSLLLTDLPAAAQQPSQTAPPSTPQVRVQTQGA
                     PTPDPNDPVQRIRDEGLNRSQVMQTLTYLTDVIGPRLTGSPQMKRANEWTRDKLTSWG
                     LQNARLEPWGPFGRGWSLRRFSAEV"
BASE COUNT          447 a          783 c          826 g          340 t
ORIGIN      
        1 agagatcgtg gcggaagccg agcggcagca gttcgtcgtc agggacgtca cgaccaaaga
       61 gcgcaagcgc aaccccgtgc cgcccttcat cacgtcgaag ctgcagcagg aggccgcgcg
      121 caagctcggc ttcgcggtga agaagacgat gatgctcgcg cagaagctct acgagggcgt
      181 cgagctgggc gccgaaggct cggtcggcct catcacctac atgaggacgg actcgacgcg
      241 cgtctccgag acggcgctcg ccgaggcgcg cgacttcatc aacgcgcagt acggcccgcc
      301 ctacctgccg gagaaggcca tccactaccg ctcgaagaag gacgcgcagg acgcgcacga
      361 ggcgatccgc ccgtcggacg tggcgcgcac gcccgactct ctggcgcgct acctcggcaa
      421 ggacgagctg cgcctgtacc gtctcatctg gcagcgcttc gtcgcctcgc agatgatgcc
      481 cgcggtcttc gaccagacga ccatcgacat cgaggccggt cgcttcctgt tccgcgcgac
      541 cgggtcggtc ttgaagttcg acggcttcct gaaggtgtac gaagagggcc gcgacgagaa
      601 gacggacgag gacgaagagg ccgcgcgcac gctgccgctc gtcgagaagg gcgagcagtt
      661 gcggctcaac gccgtgacgc ccgagcagca cttcacggag ccgccgccgc gctacacgga
      721 ggcgacgctg gtgaaggcgc tcgaagagaa gggcatcggt cggccttcga cctacgcggc
      781 catcatgacg acgatacagg atcgcgagta cgtcgagcgc gtcgagggcc gcttccaccc
      841 gacggctctg ggcttcaccg tcaacgacct tttggtcgcc agcttcgacg acctcttcaa
      901 cgagtcgtac acggcgcgga tggaggagga gctggacgag gtcgaggaag ggaagctgaa
      961 gtggaccgag gcgctgcacg agttctacgg caagttcgcc aaggacttga agaccgccga
     1021 gaccgagatg cgcaagaaga aggtcgaggc catcccgacc gacgaggtgt gcgagaactg
     1081 cggctcgccg atggtcatca agttcggtcg cttcggccag ttcctcgcct gctcgaacta
     1141 ccccgagtgc cgtaccacgc gcgaggtggc caagccgcag ggcgcgaccg ccgcggctga
     1201 agacggcgcg gcgaagcccg cgggcgttgc gggcgccgaa ggcgacggcg tcgaagagac
     1261 gtgcgacctg tgcggcaagc cgatggcctt gaagcgcggt cgcttcggcc agttcctcgg
     1321 atgcacgggt tatcccgact gccgcaacat caggaagatt agtaaagcgg gcaaggtcgc
     1381 gcccgcgccc gtcccgctcg aaggcgagac gtgcccggtg gacggcgcgc agctcgtccg
     1441 gcgtcacggc ccgtacggcg agttcgtctc gtgcgccaac tacccgacgt gcaagtacat
     1501 caagcgcgag acgacgggcg ttgcctgcgc gaagccgggc tgcaagggcg agatcgtcgt
     1561 gaagaagtcg aagcgcggca aaatcttcta cggctgctcg gaatacccga agtgcgacgc
     1621 ggtctactgg gacaagcccg tggccgagcc gtgcccgcag tgcaacgcgc cgttcctgct
     1681 tgagaagacg acgaagaagg gcaccgcgcg ctactgcgcg cgcgccgaag agtgcggcta
     1741 ccgcgaagag gcgctgccgc acgcgcccgc gccgaagagc cggtcggagg gcgatacgcg
     1801 cttcgcgcgc tgaggccggg acgggcttga cgagtcgcgc gaggggcgaa cccgcttggg
     1861 ttcgcccctc gcgcattttt tgtgggcgct tgtattttag gcgtccataa tcttaaactt
     1921 ccgtccacac agaagaacgt caccgcgcgc ccgccgcgac gcagcgcgcg cggctgccgc
     1981 caaaccttag ccatagtctt caggagccac accgtgatgc cgaaacgcag gatgaccgca
     2041 ctactgctct tctcgctgct cttaaccgat ctgcccgcgg cggcgcaaca gccgtcgcag
     2101 accgcgccgc cctcgacgcc gcaggtgcgt gtgcagacgc agggcgcgcc gacgcccgac
     2161 ccgaacgacc ccgtccagcg catcagggac gaggggctga accgctcgca ggtgatgcag
     2221 accttgacgt acctgacgga cgtgatcggg ccgcgcctca cggggtcgcc gcagatgaag
     2281 cgcgcgaacg agtggacgcg cgacaagctc acgtcgtggg gcttgcagaa cgcgcggctt
     2341 gagccgtggg ggccgttcgg tcgcgggtgg tctctgcgac gcttctcggc ggaggt
//
LOCUS       DBNGOH010000878         5961 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1234104, whole genome shotgun sequence.
ACCESSION   DBNGOH010000878 DBNGOH010000000
VERSION     DBNGOH010000878.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5961)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5961)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5961
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1234104"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1074
                     /locus_tag="AC159P_17000"
     CDS             <1..1074
                     /locus_tag="AC159P_17000"
                     /inference="COORDINATES: protein motif:HMM:NF024753.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HIR3697194.1"
                     /translation="ATVGVGHSSPVLSGGKVFVFARQGEEEVLTALDPSTGKELWKSG
                     GHAVPYEMNSAARGHGKGPKSTPVVASGRVYTLGISGVLSAHDAATGKLAWRKEFSKQ
                     FPTTSPLYGTSMSPVVEKNLVIAHVGGHDRGALTAFDAATGAVKWSFEPDGPSYASPV
                     VATLGNVRQVVTFTQKEFVSVNADSGELLWKLPAKTSYDTNCNTPVVYKDTVIFALED
                     GGIVAVRPSKQGAKWTAQEVWRNADNALYMNTPVVHGNLLFGLSARNKGQLFALDAAT
                     GKTVWQGPGRAGENASILNLAGTLLVLLDDATLLVQPATPAGYAPAARYTVADSPTWA
                     HPLVVGDRLLVKDETSLRSLALK"
     gene            1264..1707
                     /locus_tag="AC159P_17005"
     CDS             1264..1707
                     /locus_tag="AC159P_17005"
                     /inference="COORDINATES: protein motif:HMM:NF024297.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR3697195.1"
                     /translation="MKKMILSVALLAAASLAVLQPFARAQNAEEAAVRAAIEHYFRAH
                     ATGLGEHHRKVFHPEAKLFWINDGKFTQRTSEEYISGSPGSPAPDEAQRKRTVEMIDI
                     TGDAAIAKLVLDYPNAKLTDYMSLLKIDGEWRIVNKIFTREMKKK"
     gene            complement(1862..2245)
                     /locus_tag="AC159P_17010"
     CDS             complement(1862..2245)
                     /locus_tag="AC159P_17010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697196.1"
                     /translation="MDTFTDQNFRKLCDAVKGIYTDPEHVAAVGWNADTPVHHIEGAV
                     IAEIHARMEEFIGERMEVAERIRARHIPPETLGESHTRRRREEVIDFMRKHRGEGLSH
                     PDAILNQTVQAVVDHWRHEFGPDRA"
     gene            complement(2274..3089)
                     /locus_tag="AC159P_17015"
     CDS             complement(2274..3089)
                     /locus_tag="AC159P_17015"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006636 - unsaturated fatty acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HIR3697197.1"
                     /translation="MVVFHIGAVAALFMFSWPALVASLLLWWVSGSLGVGMGYHRLLT
                     HRGFKTPKAVEYLLTFCGLLALEAGPIAWVVTHRIHHAHTDAPGDPHTPRDGSWWAHM
                     GWILKGTAQRYDHAVCMRYAPDLMKDPVHRWFEKWYYVPLIVLGVVLLAVGGWSMLFW
                     GVFLRVTFNLHSTWLVNSATHLWGGRRFDTKDDSRNSWWVALLTWGEGWHNNHHAHPS
                     AARHGLVWYEVDINWWGIRTLQMLGLAKDIKLIDANGQVKGTASTRAQLKTAA"
     gene            complement(3354..3584)
                     /locus_tag="AC159P_17020"
     CDS             complement(3354..3584)
                     /locus_tag="AC159P_17020"
                     /inference="COORDINATES: protein motif:HMM:NF024835.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR3697198.1"
                     /translation="MIEIRIDELLEVRGRTFYWLAKETGISHSTLWRLKTGRAVGINF
                     PTLAQICRVLSCQPGDVLKLADEKKKVKRRST"
     gene            3989..4306
                     /locus_tag="AC159P_17025"
     CDS             3989..4306
                     /locus_tag="AC159P_17025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697199.1"
                     /translation="MSSLYAMQRANGDWFALDDHGRLRMPVFRSSSAAMHARSRHPGM
                     FLFKPMIFDERALKGLALEEDASDVCFWLVDEPFVNLSRGLSLDHAQFDQLMHETPAQ
                     PRR"
     gene            4402..4623
                     /locus_tag="AC159P_17030"
     CDS             4402..4623
                     /locus_tag="AC159P_17030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697200.1"
                     /translation="MVTDNLLGAAVRIRRGWHGGRTGKVMVCVRHGGFTTSYSVIKVQ
                     LDGSGQVVNINTDEELEKLADLPGGSAVE"
     gene            4687..5448
                     /locus_tag="AC159P_17035"
     CDS             4687..5448
                     /locus_tag="AC159P_17035"
                     /inference="COORDINATES: protein motif:HMM:NF013868.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HIR3697201.1"
                     /translation="MFTRHVIMQLKPDSAAALALIFENEVIPLLRGQKGLRHDDTFIS
                     PEISEAVMNSYWDTEGYADAYVRAAYPAVLKALAGVLEGVPQVETFVISSSTFHRLTS
                     YRREAYRTNRLGRGVGTSAPLAHYAKPKPKSQGYGMVNCINERRVGSVTVLDLDGEIR
                     TGGSRIALHDAIGALLGEGRNRILLNLNGLSTIDASGLGELISSHVALHESGGQLRLL
                     HLTRALREMMTMTKILTIFDVHEDEAEALAGFGSQ"
     gene            5515..5763
                     /locus_tag="AC159P_17040"
     CDS             5515..5763
                     /locus_tag="AC159P_17040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697202.1"
                     /translation="MSFIRESLMYALLWGVGLLTAVIAVWQFYVFVGFRDAQGSLDVQ
                     GGGLHLWLAIGAAAVACACVCAGIFRRINKTEEFHITS"
BASE COUNT         1137 a         1910 c         1824 g         1090 t
ORIGIN      
        1 gcgaccgtcg gcgtcggcca ctcgtcgccg gtcttgtctg gcggcaaggt cttcgtcttc
       61 gcgcggcagg gcgaggaaga ggtgttgacg gcgctcgacc cctcgaccgg caaggaactc
      121 tggaagagcg gcggccacgc cgtcccttac gagatgaact ccgccgcgcg cggccacggc
      181 aaggggccga agtccacgcc cgtggtcgcg tcggggcgcg tctacacgct cggcatctcg
      241 ggcgtgctct cggcgcacga cgccgcgacc ggcaagctcg cgtggcgcaa agagttctcc
      301 aaacagttcc ccacgacctc gccgctctac ggcacgtcga tgtcgcccgt cgtcgagaag
      361 aacctcgtca tcgcgcacgt cggcggccac gaccgaggcg cgctcaccgc cttcgacgcc
      421 gcgaccggcg ccgtcaagtg gtctttcgag cctgacggcc cgtcctacgc ctcgcccgtc
      481 gtcgcgacgc tcggcaacgt ccggcaggtc gtcaccttca cgcagaagga gttcgtctcc
      541 gtcaacgcag actccggcga gctgctctgg aaactgcccg ccaagacctc ctacgacacc
      601 aactgcaaca cgcccgtcgt ctacaaagac acggtcatct tcgcgctcga agacggcggc
      661 atcgtggccg tccgcccgtc caagcaaggc gcgaagtgga cggcgcaaga ggtgtggcgc
      721 aacgccgaca acgcgctcta catgaacacg ccggtcgtac acggcaacct cctcttcggc
      781 ctctcggcgc gcaacaaggg gcagctcttc gccctcgacg ccgcgaccgg caagaccgtc
      841 tggcaggggc cgggccgcgc gggcgagaac gcctcgatcc tcaacctcgc gggcacgctc
      901 ctcgtcctcc tcgacgacgc gaccctcctc gtccagcccg ccacgcccgc gggctacgcg
      961 cccgccgcgc gctacaccgt cgccgacagc cccacctggg cgcaccccct cgtcgtcggc
     1021 gaccgcctgc tcgtcaaaga cgagaccagc ttgcgctcgc tcgcgctcaa gtgatcgcgg
     1081 gatgcggacg gactgcggcc cgcggatcca aaaagcgccg acggcggacg aacgttcctg
     1141 aagcgccgcc ttcatcaacc atcaaggatg ttcgaggagc agcccgctct tcaacaatca
     1201 aaggacgcat aaaaaaaggg ccgccgcccg agcgcccgtc aacacgtgga acaggagagt
     1261 tgaatgaaaa agatgattct gtccgtcgcc cttctcgccg ccgcgtcgct cgccgtccta
     1321 cagcccttcg cgcgcgcgca gaacgccgag gaggccgccg tccgggccgc catcgaacac
     1381 tacttccgcg cccacgccac cggcctcggc gagcaccacc gcaaggtctt ccacccagag
     1441 gccaaactct tctggatcaa cgacggcaag ttcacgcagc gcaccagcga agagtatatc
     1501 tccggctcgc ccggctcgcc cgcgccggac gaggcccagc gcaaacgcac cgtcgagatg
     1561 atcgacatca ccggcgacgc cgccatcgcc aaactcgtcc tcgactaccc caacgccaaa
     1621 ctcaccgact acatgtcgct cctcaagatc gacggcgagt ggaggatcgt caataaaatt
     1681 ttcacccgcg agatgaaaaa gaagtagaga tcaagaggaa gaggggaaag cagttctggt
     1741 catgaaggcg agggcggagg ttgtctgctc tcgccgggtg atgcgcgcgt ttcggggtcg
     1801 gcaagcgtgt tgagggtttg ctgcttctcg cgctcgacct gcaacgcggg gtcgggccgg
     1861 atcacgctcg atccggcccg aattcatggc gccagtgatc gaccacggcc tggactgtct
     1921 ggttaaggat ggcgtccggg tgagagagac cttcgccacg gtgtttcctc atgaaatcga
     1981 tgacctcttc cctgcgccgc ctcgtgtggg attcgccgag ggtttcgggg gggatgtgcc
     2041 gcgcacgaat cctctccgcg acctccatcc tctccccgat gaactcttcc atccgggcat
     2101 gtatctcggc gatcaccgcc ccttcgatat gatgaacggg cgtatccgcg ttccagccga
     2161 cggcggcgac gtgttcgggg tcagtgtaga tgcccttcac cgcgtcgcat aatttcctga
     2221 agttctgatc ggtaaatgtg tccatgaccg gttgagcgag tagcttcaca gtgttatgcc
     2281 gccgtcttga gttgcgcccg cgtcgaggcg gttcccttga cttgaccgtt ggcgtcgatc
     2341 agcttgatgt ccttcgccag ccccagcatc tggagcgtgc ggatgcccca ccagttgatg
     2401 tccacttcgt accacacgag accgtgcctc gcggcggacg ggtgcgcgtg atgattattg
     2461 tgccagcctt cgccccacgt gagcagcgcc acccaccaac tgttacgcga atcgtccttc
     2521 gtgtcgaacc gccgcccgcc ccagaggtgg gtcgccgagt tgacgagcca cgtcgagtgt
     2581 aagttgaacg tcacgcgcag gaagacgccc cagaacagca tcgaccagcc gccgacggcc
     2641 agcagcacca cgccgagcac gatgagcggc acgtaatacc acttctcgaa ccagcggtgc
     2701 acggggtctt tcatcaggtc gggggcgtaa cgcatgcaca ccgcgtggtc gtaacgctgc
     2761 gccgtgccct tcagaatcca gcccatgtgc gcccaccaac tcccgtcgcg cggcgtgtgc
     2821 gggtcgcccg gcgcgtccgt gtgcgcgtgg tggatgcggt gcgtcacgac ccaggcaatc
     2881 ggccccgctt cgagggccag caggccgcaa aaggtcagga gatactcgac cgctttcggc
     2941 gttttgaagc cgcggtgcgt caacagccga tggtagccca tgccgacccc gaggctcccg
     3001 ctcacccacc agagcagcag actcgccacg agcgccggcc aactgaacat gaagagcgcc
     3061 gccaccgcgc cgatatggaa aaccaccatc gtgatgtaag tcggccaatt gatgccggtc
     3121 cttcgttgaa tatcggtgat cgcttcgtga cgcattcgag ccccctgttg ttaccaagat
     3181 gtgtgcctct actttgataa cacaggaggg cggattgtgc cggttcgctt gtaatagtta
     3241 tgtaatatcg agacctgaag aacagcgcga cggctgcgcg gcccgaccaa cgacctttgt
     3301 acgtttgcgc gcacggcgcc gttatccgcc tcgcgttgcg attcgttccc gcctcacgtg
     3361 cttcgtctct taaccttctt tttctcgtcc gccagcttca acacgtcacc cggctgacaa
     3421 ctgagcaccc ggcagatctg cgccagggtg ggaaaattga tgcccaccgc cctgccggtc
     3481 ttcaaccgcc acaacgtcga gtggctgatg cccgtctcct tcgccagcca gtagaaagtc
     3541 cgcccgcgca cttccagcaa ctcgtcgatt cggatttcga tcacgatgcg ccccctcttc
     3601 ggatattcgc tgtgtattat atccgaaatg cagcgattgc agaatatatt tcacatttga
     3661 catatagagt cgcctcgcgt actgttgtac agaaccgagt ctagctcact cccttctcag
     3721 atcttctgga actacccaga ccacctgacg cccacggctc aactaccacg aagcgaaagg
     3781 cgtttacctt gcccgcccgg tctcgaacac gagccgattt ccgtatttcc gcaatacagg
     3841 aaggtgataa gcctcggggc aatccccggg ggatgggtgg cggaaggggc gcttcgtgaa
     3901 agtctgacgg ctcggcgctc cgcctccgaa acggcacaca acttcgacta cggctcttga
     3961 acgtgcgtcc tgcagtgagg agacagtaat gagcagtcta tatgccatgc agcgcgccaa
     4021 cggcgactgg ttcgcgctcg acgatcacgg tcgcctgcgc atgccggtct ttcgcagcag
     4081 cagcgccgcg atgcacgcgc gcagccgtca tccggggatg ttcctcttca agccgatgat
     4141 cttcgacgag cgtgcgctca agggactcgc gttggaagag gacgcgagcg acgtgtgctt
     4201 ctggctggtg gacgagccgt tcgtcaacct gagccggggt ctttcactgg atcacgcgca
     4261 gttcgaccag ctcatgcacg agacccctgc gcagccgcgg cgttgagacc gggggcagca
     4321 gatcgaacgg cggacagtgt gccgcgaagg cgacgggacg agggttgctc gggacgcggt
     4381 tccgttgcga ggaaggtgaa tatggtcacg gataatttgc tgggcgcggc agtcagaatc
     4441 cgtcggggct ggcacggggg cagaaccggg aaagtaatgg tctgtgtccg gcacggcggc
     4501 ttcaccactt catacagcgt gatcaaagtt cagctcgacg ggagcggtca ggtcgtgaac
     4561 ataaacaccg acgaagagtt ggagaaactc gccgacctgc cgggcggttc ggctgtcgaa
     4621 taacgcgggc cgtgttggtc gaacttacag ccgccggcgt gcgtctctat taccgaggag
     4681 tcaagaatgt tcacccgcca cgtcatcatg caactgaagc ccgattccgc cgccgcgctc
     4741 gcgctgattt tcgagaacga ggtcatcccg ctgctgcgcg ggcagaaggg cctgcgccac
     4801 gacgacacct tcatcagccc ggaaatctcg gaggccgtca tgaacagcta ctgggatacg
     4861 gaagggtacg cggacgccta cgtccgtgcg gcttacccgg cggtgctcaa ggctctggcg
     4921 ggcgtgttgg agggggtgcc gcaggtggag acgttcgtga tttccagctc gacgttccac
     4981 aggcttacgt catatcgccg cgaggcgtat cgaacgaata gattggggag gggcgtcgga
     5041 acgtccgccc cgctcgcgca ctacgccaaa ccgaaaccga aatcgcaagg gtatgggatg
     5101 gttaattgta tcaacgagcg ccgggtcgga agcgtgaccg tccttgacct ggacggggag
     5161 ataaggaccg gcgggagccg tatcgcactg cacgacgcca tcggcgcgct gctgggagag
     5221 gggcgaaatc ggattctgtt gaatctcaac gggctttcca ccatcgacgc gagcgggctg
     5281 ggcgagttga tctcaagtca cgtcgcactg cacgagagcg gcgggcagtt gagactcctt
     5341 catctgacgc gggcgctccg tgaaatgatg acgatgacga agattctcac catcttcgac
     5401 gttcatgaag acgaggcgga agccctcgcc ggcttcggta gtcaatgaat agcagcaccg
     5461 gttcatcgtg cggctgtccc ggcttaactt ggaaacaact ggcggaggtg ccacatgagt
     5521 tttatcagag agagtttgat gtatgccctg ctgtggggtg tcggtctgtt gacggcggtg
     5581 atcgcggtct ggcagttcta cgtgttcgtc gggttcaggg acgcgcaggg gtctctggac
     5641 gtgcagggcg gggggctgca cctgtggctg gcaatcggcg cagccgctgt ggcctgcgcg
     5701 tgcgtgtgcg cgggcatctt ccgccggatt aataagacgg aagagttcca catcacttcc
     5761 tgagcggcgc tcgccaagac ggatgccgtg aggcgcaggt tctcacgcgc gcgggaatta
     5821 tcgaagcgtt caccgcgccc gccggatgtc atcgatcgga gcttgtatgg atccagctaa
     5881 aggagtgtca ggatgaaccc gaacagcatg gtgtttctgc tgctcataat cttaggcggg
     5941 gtgcccatca tcatactgtt g
//
LOCUS       DBNGOH010000879         2400 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3066692, whole genome shotgun sequence.
ACCESSION   DBNGOH010000879 DBNGOH010000000
VERSION     DBNGOH010000879.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2400)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3066692"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..141)
                     /locus_tag="AC159P_17045"
     CDS             complement(<1..141)
                     /locus_tag="AC159P_17045"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003803870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HIR3697203.1"
                     /translation="MRVGILTGGGDCPGLNAVIRGAVLKGVISHDAEFVGFRKGWKGV
                     VEG"
     gene            242..1078
                     /gene="tsaB"
                     /locus_tag="AC159P_17050"
     CDS             242..1078
                     /gene="tsaB"
                     /locus_tag="AC159P_17050"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HIR3697204.1"
                     /translation="MSNESNFNPLSGAQGGTKNAREGAGEAPRPLILSIDTASAARSL
                     AVTRGAEPLSLHVCGQISANSATVLGDIDRVLCAASVGVQEVELFALAKGPGSFTGLR
                     AGLATLKALAVTLRRPAVGVPTLHAVAHAAASSSHGGSVLALMPAGRGELFAQHSSLG
                     GDGTIREHGPPSHVSPAELIARARGIEGRLTWAGVGAEKFRALIEEAARAEGVEVVEG
                     GAVLPEEKGGRVWAVAPDVGALACVIAALAATRYRPGEDFRGDKLKALYVRPADARIG
                     GA"
     gene            1141..1593
                     /locus_tag="AC159P_17055"
     CDS             1141..1593
                     /locus_tag="AC159P_17055"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3697205.1"
                     /translation="MTEHDLLEVVAIEESCGLSLWGWDAYRAELDRPESIMLVARREA
                     PTHMSEQRLRGFIAARVQAGELHVNNIGVREGERRQGVGGFLLGRALRVGAHYGAGAA
                     LLEVRVGNAAAQALYRAHGFVVTGRRRGYYKEPREDALVMARRLETEA"
     gene            1721..1963
                     /locus_tag="AC159P_17060"
     CDS             1721..1963
                     /locus_tag="AC159P_17060"
                     /inference="COORDINATES: protein motif:HMM:NF016231.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HP1242/YdcH family protein"
                     /protein_id="HIR3697206.1"
                     /translation="MDMTNTDSLKEQLMQSDPEFRELAREHGRYEERLSQLTALAYPS
                     DEEQLEEVTLKKKKLAVKDQMHAIMLRHRQPESTAH"
     gene            2115..>2400
                     /locus_tag="AC159P_17065"
     CDS             2115..>2400
                     /locus_tag="AC159P_17065"
                     /inference="COORDINATES: protein motif:HMM:NF014696.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HIR3697207.1"
                     /translation="MFVRDAIPWVLIPVLAALFLLWLGLWWLSVPLLLVAAFMAYFFR
                     DPRRVVPAGPDVVVSPADGRVTRVEKVSPADPVSATVVSIFLSPFDVHINR"
BASE COUNT          400 a          777 c          844 g          379 t
ORIGIN      
        1 gccctcgacg acgcccttcc accccttgcg gaacccgacg aactcggcgt cgtgggagat
       61 gacgcccttc aacaccgcac cgcggatgac cgcgttgagt ccggggcagt cgccgccgcc
      121 cgtaagaatg ccgacgcgca tgatcttcta acccaccttg ccgcagatgc cgagccgcgt
      181 aaagaaaacg tgatttgatt tggttacggg gacgcaccta taatcacccg gtaacagtgg
      241 catgtcaaac gagagcaact tcaatccgct ttccggcgcg caaggtggca cgaagaacgc
      301 gcgggagggc gcaggggagg cgccgcggcc cctcatcctc agcatcgaca cggcgtccgc
      361 ggcgaggagc ctcgccgtca cgcgcggcgc cgagccgctc tcgcttcacg tctgcggaca
      421 aatttcggcg aactccgcga ccgtcctcgg ggacatcgac cgcgtgctct gcgcggcctc
      481 ggtcggcgtg caagaggtcg agctgttcgc gctcgcgaag gggccgggga gcttcacggg
      541 gctgcgggcc gggctggcga cgttgaaggc gctcgcggtg acgctgcgca gacccgccgt
      601 cggcgtcccg acgctgcacg cggtcgcgca cgcggcggcg tcgtcctcgc acgggggaag
      661 cgttttggcg ctgatgccgg cgggccgcgg cgagctgttc gcgcagcatt cgagcttggg
      721 cggcgacggc acgatccgag agcacggccc gccgtcgcac gtctcgccgg cggagttgat
      781 cgcgcgcgcg cgcggcatcg aaggcagact gacctgggcc ggggtcggcg cggaaaagtt
      841 tcgcgcgttg atcgaggagg cggcgcgcgc cgaaggcgtc gaggtcgtcg aaggcggcgc
      901 ggtgcttccg gaggagaagg gcgggcgtgt gtgggcggtc gccccggatg tgggcgcact
      961 cgcctgcgtt atcgcggcgc tggcggcgac gagataccgt ccgggtgaag acttccgggg
     1021 tgataaactg aaggccctct acgtccggcc cgcggacgcg agaatcggcg gcgcgtgagt
     1081 gtgatgacca gagtgatcgc tcccttcgag caagagtgct cgactttcca tatcgactcg
     1141 atgacggaac acgacctgct ggaagtggtc gccatagagg agtcgtgcgg cctgagtctg
     1201 tggggctggg acgcgtaccg cgcggagctc gaccgccccg aatccatcat gctcgtcgcg
     1261 cggcgcgagg cgccgacgca catgtcggag cagcggcttc gcgggttcat cgcggcccgc
     1321 gtgcaggcgg gcgagctgca cgtcaacaac atcggcgtcc gggagggcga gcggcggcaa
     1381 ggggtcggag gctttctgct gggccgcgcg ctgcgggtcg gcgcgcacta cggcgcgggc
     1441 gcggccctcc tggaagtgcg cgtggggaac gccgcggcgc aggccctcta ccgggcgcac
     1501 ggcttcgtcg tcacggggcg gcggcgcggt tactacaagg agccgcgtga ggacgcgctc
     1561 gtcatggcgc ggcggctcga aacagaggct tgattaggcg gcccgagatg ggctaccatt
     1621 ttccgcacga acaaaggcgc agcccctctg gtggaaaaat gattcatccg ttgccccgtc
     1681 tttttcaaat ctttcgcgtc tgaaaggaga ccaccccgaa atggacatga ccaacacgga
     1741 cagcctgaag gagcagttga tgcagagcga cccggaattc cgcgagctgg cgagggagca
     1801 cgggcgttac gaggaacgcc tcagccagct cacagcgctc gcctacccga gcgacgaaga
     1861 gcagctcgaa gaggtcacgc tgaagaagaa aaagctggcc gtcaaagatc agatgcacgc
     1921 catcatgttg cgccaccggc agccggagag cacggcccac tgagcgaggg cgagcgggca
     1981 aggggctttc ggcaggcgcc tcaacttact agcacggaac ggggacgctt cacacacgct
     2041 gggcgccccc gcttctttta tcgtccacgg aaacgcgcac ccgtcggaat gaacgacaga
     2101 ggcacctgac ttttatgttc gtgagagacg cgatcccctg ggttctgatc cccgtcctgg
     2161 ccgcgctgtt tctgctctgg ctcggcctct ggtggctgtc ggtcccgctc ctgctcgtcg
     2221 cggccttcat ggcctacttc ttccgcgacc cgcgccgcgt cgtgcccgcg gggccggatg
     2281 tcgtcgtctc gccggcggac ggtcgcgtga cccgcgtcga gaaggtctcg ccggcagacc
     2341 ccgtgtcggc gaccgtcgtc agcatcttcc tctcgccgtt cgacgtgcac atcaaccgcg
//
LOCUS       DBNGOH010000880         6279 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4348603, whole genome shotgun sequence.
ACCESSION   DBNGOH010000880 DBNGOH010000000
VERSION     DBNGOH010000880.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6279)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6279)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6279
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4348603"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..310)
                     /locus_tag="AC159P_17070"
     CDS             complement(<1..310)
                     /locus_tag="AC159P_17070"
                     /inference="COORDINATES: protein motif:HMM:NF014375.7"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HIR3697208.1"
                     /translation="MKILLYNPDNGVTRNFMPHLWMFLLQAITPPGHEVLLIDGNARA
                     LTDEELVRYVREENIELVGIGAMTRMIAKAYRVADALRAAGVRVVMGGPHVTECPDEA
                     L"
     gene            complement(668..2008)
                     /locus_tag="AC159P_17075"
     CDS             complement(668..2008)
                     /locus_tag="AC159P_17075"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3697209.1"
                     /translation="MDSLRAELRRPATFGPSPLPFFYGWVSVALAALAMVGTLPGRTQ
                     GLGLITEPLIADLGIDRVVFARVNFWATLVGALFALGVGRLVDRFGSRVVLAVVAAAL
                     GAVVLLMSGAEGLAALAVLVTLTRGVGQSALSVVSITMVGQWFVRRLSVAMAVYTVAL
                     SIGFMVAFPLVGAVVGGYGWRAAWAGIGAALLLGLAPLAGFFGRRSPEACGLAPDGAG
                     GAGGGDAVRGADDDTAVVVHRPREAGASRTADAGEGYTLRQALSTPAFWVFGLASAVY
                     GLTASGIGLFNESILAERGFDAATYYNTLVITALTALAGNFLGGWLMGKWTMSRLMAL
                     AMGLLAGSLAGLPHVRTQAHVAAYAVVMGLAGGFVIVIFFSFWSRAFGRTHLGKIQGA
                     AQALTVLASATGPLLLAECVTRTGSYAAMFYALTLVVAALGAGAWFVPLPRARA"
     gene            complement(2037..2591)
                     /locus_tag="AC159P_17080"
     CDS             complement(2037..2591)
                     /locus_tag="AC159P_17080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697210.1"
                     /translation="MGGGEIITRVLVWFAVAGYVAGAGAFALARGRRGWDAWARAAWS
                     VGCAAMLAHVACAFHFYHGWSHAAAYRDTARQTAEVTGLVWGGGLYFNYALVAAWAAD
                     VVCWWRRGLDAYRRGPWPLTVAWHAFLIFMFFNATVVFGKGAARWAGLCACAALGGAW
                     WLGATARGVGPGGANKKGLTVVED"
     gene            complement(2591..3187)
                     /locus_tag="AC159P_17085"
     CDS             complement(2591..3187)
                     /locus_tag="AC159P_17085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697211.1"
                     /translation="MTNSSERADGRAAGSVVVPSLGRAALVGGVGFCVVSLCVFGTVA
                     FAERWMYARLGQLGAYAAWTALFIALGGGVLGALVAGRWRLPRFYVLFGAAFFAYAAG
                     WVGAYFVLRGAAGEWAGSVLGSVLMGTVFAAGFGVLREALKLSAVLFTANSFGYFLGA
                     ALNETFGGAAGMLLWGAVYGLALGAGLGAVLHLAQTKS"
     gene            complement(3218..3778)
                     /locus_tag="AC159P_17090"
     CDS             complement(3218..3778)
                     /locus_tag="AC159P_17090"
                     /inference="COORDINATES: protein motif:HMM:NF019614.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HIR3697212.1"
                     /translation="MPNYVAFLRAINVGGHTVKMQHLRRLFEAQGFSGVETFIASGNV
                     IFDSASANAKALERKIESSLREALGYEVATFLRSTGELLNVARHKPFAAAELEREGNV
                     LYVAFLADRPGEEATRKLLSCANEVDDFHVAGREVYWLRRTKVGESKFSGALLERTLG
                     AQATVRNSTTVGKLAAKYASGGGTIK"
     gene            4012..4233
                     /locus_tag="AC159P_17095"
     CDS             4012..4233
                     /locus_tag="AC159P_17095"
                     /inference="COORDINATES: protein motif:HMM:NF041212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxx-star family glutaredoxin-like
                     (seleno)protein"
                     /protein_id="HIR3697213.1"
                     /translation="MGLVLYVKPGCPYCQQARDHYRAAGEAWTEYDAQNDRARRKEMF
                     ELSGGDPTVPCVVRDGRYVQSGWGDPLRG"
     gene            4447..>6279
                     /locus_tag="AC159P_17100"
     CDS             4447..>6279
                     /locus_tag="AC159P_17100"
                     /inference="COORDINATES: protein motif:HMM:NF019334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR3697214.1"
                     /translation="MVGQTLLNYRITEKLGAGGQGTVYKAVDERLGRTVVIKILSPEL
                     TAREVNLKRFEREAQLASSLDHPNICTIYGLHELDGLHFIAMQYIRGRNVREVVNGRP
                     LALNSALSIAYQVTDALTAAHAEGIIHRDIKAGNVMVTEAGLVKVLDFGLAKLLEDEA
                     ESKRKGKGGEQIHLTELGVPYGTATYAAPEQATGQKVDHRADIFSTGVLLYEMLAGIW
                     PFQGKTSVEVRYAVLHDTPQALAEIRKDNPPPRLQEIVDRALAKRPDQRYQRMSELRE
                     DVRAAMREVAAASGVEDNSAAGMPGDLVPLSKTRHGGQRGLGRSFRRWWRSLTGSEAQ
                     PTSLPPGMKPATATSNAGGASTASSAPPPAAPAELHDTPHASKFEEQKSIAILPFRNL
                     SNDPESSFYEFSLADAVITELGRLRSLVVRPSSLIVKYQGKQLDPREVGRELKVSAIM
                     EASFLRAGEHLRVNAQLVDVYTGDILWSDRIDASAQDIIALQDLIAQRIAKGLKVEPA
                     PAEKAEAAGPATRNAAAYEEYLRGRDYFAKFLFGTLDPEECDRAIAHFQRAIQLDHSF
                     ALAHSGLGACFANRVLKGLGGTEDYELAENAFQRALELDPDVVEA"
BASE COUNT         1134 a         2320 c         1967 g          858 t
ORIGIN      
        1 cgagggcctc gtccgggcat tcggtgacgt gcgggccgcc catcaccacg cgcacgcccg
       61 cggcccgcag cgcgtcggcc acgcggtagg ctttggcgat catccgcgtc atcgccccga
      121 tgccgaccag ctcgatgttt tcttcgcgca cgtagcggac gagttcttcg tcggtcagcg
      181 cgcgcgcgtt gccgtcgatg agtaagacct cgtggccggg cggcgtgatg gcttgcagca
      241 ggaacatcca gaggtgcggc atgaagttgc gcgtcacgcc gttgtcgggg ttgtacagta
      301 aaatcttcat cgaagtcctt tacgaaaaaa atccggcgtg tctcgcccac ggggcgggaa
      361 tgtagagatt cctgatgcct tgggtttcca aaggggtgct acgccccttc gtatccgacg
      421 gaggcgggga gcgccccgcc cgcaaaggcg gagcctgcgg cactgtttag ccgccgcaga
      481 gtttactccg gtcaggcgat taaagccagc gagctaaggt tagagaccgt acaacaatgt
      541 tcggatgtga agaaagcagc cgaaaaagcg ttgccttcag gccgacagag cgcgagcaga
      601 gggctttcgc aggagtttga attttatccc ggcccgccgg gttgacgaaa gtcggcgacg
      661 cccggactca cgcccgcgcg cgcggcaacg ggacgaacca cgcgcccgcg ccgagcgccg
      721 ccacgaccag cgtcagcgcg tagaacatcg cggcgtaaga gcctgtccgc gtcacgcact
      781 cggcgaggag cagcggcccg gtcgcggagg cgagcacggt cagcgcctgc gccgcgccct
      841 gtatcttgcc gaggtgcgtg cgcccgaagg cgcggctcca gaagctgaag aagatgacga
      901 tgacgaagcc gcccgccagc cccatcacga cggcgtaggc cgcgacgtgc gcctgcgtcc
      961 gcacgtgcgg cagcccggcg agcgaccccg ccagcagccc catcgccagc gccatcaggc
     1021 ggctcatcgt ccacttcccc atcagccagc cgccgaggaa gttgcccgcg agcgccgtca
     1081 gcgcggtgat gaccagcgtg ttgtaatagg tcgcggcgtc gaagccgcgc tcggcgagga
     1141 tggattcgtt gaacagtccg atgccggaag ccgtcagccc gtagaccgcg ctcgccagac
     1201 cgaagaccca gaaggctggt gtcgagagcg cctgccgcag cgtgtaacct tcgcccgcgt
     1261 cagccgtgcg cgaggcaccc gcttcgcgcg gcctgtgaac gacgaccgcc gtgtcatcgt
     1321 ctgcgccgcg caccgcgtcg ccgccgcccg cgccgcccgc gccgtcgggc gcgaggccgc
     1381 acgcctcggg cgagcgccgc ccgaagaagc ccgcgagcgg cgcgagtccc aacagcagcg
     1441 ccgccccgat cccggcccat gccgcgcgcc agccgtagcc ccccacgacc gcgcccacca
     1501 acgggaaggc gaccatgaac cctatgctca gcgccaccgt gtagacggcc atcgccacgc
     1561 tcagccgccg cacgaaccac tgcccgacca tcgtgatgct gacgaccgac agcgcgctct
     1621 gccccacccc gcgcgtgagc gtgacgagca ccgcaagcgc ggccagcccc tcggcgccgc
     1681 tcatcaaaag gacgaccgcg ccgagcgccg ccgccacgac ggcgagcacg acgcggctgc
     1741 cgaagcgatc aaccagccgc ccgacgccga gcgcgaagag cgcgccgacg agcgtcgccc
     1801 agaagttgac gcgcgcgaag acgacgcggt cgatcccgag gtccgcgatg agaggctccg
     1861 tgatgaggcc caacccttgc gtgcgaccgg gcagcgtccc gaccatcgcc agcgccgcca
     1921 gcgccacgct cacccacccg taaaagaaag ggaggggcga cgggccgaac gtcgccggtc
     1981 ggcgcagctc cgcgcgtaga gaatccatca agctctgtct tccatcaaag tcaggttcag
     2041 tcttctacga ccgtcagccc cttcttgttc gccccgcccg gcccgacgcc gcgcgccgtc
     2101 gcgccgagcc accacgcgcc gccgagcgcc gcgcacgcgc acaaacccgc ccagcgcgcg
     2161 gcgcccttcc cgaagacgac cgtggcgttg aagaacatga agatcaggaa tgcgtgccac
     2221 gcgaccgtga gcggccacgg cccgcggcgg taagcgtcaa gcccgcgccg ccaccaacac
     2281 accacgtccg cggcccacgc ggcgacgagc gcgtagttga agtacaaccc gccgccccac
     2341 acgaggcccg tcacctccgc cgtctgccgc gccgtgtcgc ggtacgccgc cgcgtggctc
     2401 cacccgtgat aaaaatggaa cgcgcacgcc acgtgcgcca acatcgctgc gcaccccaca
     2461 ctccacgccg cccgcgccca cgcgtcccac ccgcgccgcc cgcgcgccag cgcgaacgcc
     2521 ccggcccccg ccacgtaccc cgcgaccgcg aaccacacca gaaccctcgt gatgatctcg
     2581 ccgccgccca tcaactcttc gtctgcgcca gatgcagcac cgcgccgagt cccgcgccca
     2641 gcgccagccc gtacaccgcg ccccacagca gcatccccgc cgcgccgccg aacgtttcgt
     2701 tgagcgccgc gccgaggaag tacccgaacg agttcgccgt gaacaacacc gccgacaact
     2761 tcaacgcctc acgcaacacg ccgaaccccg ccgcgaacac cgtccccatc agcaccgacc
     2821 ccagcaccga acccgcccac tcgcccgccg cgccccgcaa cacgaagtac gcgcccaccc
     2881 accccgccgc gtacgcgaag aacgccgcgc cgaacagcac gtaaaacctc ggcagccgcc
     2941 accgaccggc gacgagcgcg cccaacaccc cgccgcccaa cgcgatgaac aacgccgtcc
     3001 acgccgcgta cgcgcccaac tgtccgagcc gcgcatacat ccacctctcc gcgaacgcca
     3061 ccgtgccgaa cacgcacagg ctcaccacac agaaacccac cccgcccacc agcgccgcgc
     3121 gccccagcga agggacgaca acggaacccg ccgcccgacc gtcagccctt tcggaactat
     3181 tcgtcatcgc ttgccgcacc tccgctcatg aactgcgtca cttgatcgtg cctccgcccg
     3241 acgcgtactt cgccgcgagc tttccgaccg tggtcgagtt tctgacggtc gcctgcgcgc
     3301 cgagcgtcct ttcgagcagc gcccccgaaa acttcgattc gccgaccttc gtgcggcgga
     3361 gccagtagac ctcgcgcccc gcgacgtgaa agtcgtcaac ctcgttggcg cacgacagga
     3421 gcttgcgtgt cgcctcttcg ccgggcctgt ccgcgaggaa ggcgacgtac aggacattgc
     3481 cttcccgttc gagttccgcc gccgcgaagg gcttgtgacg cgcgacgttc agcaactcgc
     3541 ccgtcgatct caggaacgtc gccacctcgt agccgagcgc ctcgcgtagc gaactctcga
     3601 tcttccgttc gagcgccttc gcgttggcgg acgccgaatc gaagataacg ttgccgctgg
     3661 cgatgaacgt ctcgacgccg gagaacccct gcgcctcgaa caggcggcgg aggtgctgca
     3721 tcttgacggt gtggccgccg acgttgatgg cccgcaggaa ggcgacgtag ttgggcatgc
     3781 gtgtgttcgc ggtctatcgc gcgcgcgcac gtcagccggc ggcgtgcgcc cggctacgca
     3841 tcatacgccg aatgaattcg ttatcaagac gtgctatgta aaaacctctc tttacgatta
     3901 atcttgtccc gccacgtccg cggcggcccg actttcaccg cacgtgcttc ccggcggcgg
     3961 cgggcgtgcg ctacactggc gtcaaagcac acaagggagg agacaggatt agtgggactg
     4021 gtgttgtacg tgaagccggg gtgcccgtat tgccagcagg cgcgcgatca ctaccgggcg
     4081 gcgggcgagg cgtggacgga gtacgacgcg cagaacgaca gggcgcggcg caaagagatg
     4141 ttcgaacttt cagggggcga cccgacggtg ccgtgcgtcg tgcgcgacgg gcgctacgtc
     4201 cagtccgggt ggggcgaccc gctgcggggt tgaacggtgc acgattgagg cgccgtccgt
     4261 gagacggccc agacgattgc ggattttgga ttgcggattg cggattgttt tgatttggcg
     4321 cgcggcggga ctacaatgcg gcgcgcgccg gcctcgccgc gctgacgacg gaagtctacc
     4381 gtgtctggac cgttaacccg aaatccgcaa tcaaaaatcc gcgatccgca atcgaaaatt
     4441 cctttcatgg ttggtcagac gcttcttaac taccgcatca cggagaagct cggcgcgggc
     4501 gggcagggca cggtctacaa ggccgtggac gagcggctcg ggcgcacggt cgtcatcaaa
     4561 atcctctcgc ccgagctgac cgcgcgcgag gtcaacctca agcgcttcga gcgcgaggcg
     4621 cagctcgcct cctcgctcga ccacccgaac atctgcacca tctacgggct gcacgagctg
     4681 gacggcctcc acttcatcgc catgcagtac atccggggcc ggaacgtccg cgaggtcgtc
     4741 aacggtcgcc cgctcgccct caacagcgcg ctctccatcg cctatcaagt caccgacgcg
     4801 ctgacggcgg cgcacgccga gggcatcatc caccgcgaca tcaaggctgg caacgtgatg
     4861 gtgaccgagg cggggctggt caaagttctg gacttcggtc tggcgaagct gctggaggac
     4921 gaggcggaga gcaagcgcaa gggcaagggc ggcgagcaga tacatttgac ggagctggga
     4981 gtcccgtacg gcaccgcgac ctacgccgcg cccgagcagg ctacggggca gaaggtcgat
     5041 caccgcgccg atatcttctc gaccggcgtg ctgctctacg agatgctcgc gggcatctgg
     5101 ccgttccagg ggaagacgag cgtcgaggtg cgctacgccg tcctgcacga cacgccgcaa
     5161 gccctcgccg aaatcaggaa agacaacccg ccgccgcgcc tgcaagagat cgttgaccgc
     5221 gcgctggcca aaaggcccga ccagcgttac cagcggatga gcgagctgcg cgaagacgtg
     5281 cgcgcggcga tgcgcgaggt ggccgccgcg tcgggcgtcg aagacaattc ggcagcaggg
     5341 atgccgggcg atctggtgcc gctcagcaag acccgccacg gcggtcagcg cggcctcggt
     5401 cgctcgttcc gccgctggtg gcgcagcctg acgggaagcg aggcgcagcc cacgtccctg
     5461 ccgcccggca tgaagcccgc gaccgcgacc tcgaacgcgg gcggcgcctc gaccgcctcc
     5521 tcggccccgc cccccgccgc cccggcagag cttcacgaca cgccgcacgc cagcaagttc
     5581 gaagagcaga agtcgattgc catcctcccc ttccgcaacc tgtcgaacga ccccgagtcg
     5641 agcttctacg aattctcttt ggccgacgcc gtcatcaccg aactcggtcg gctgcgctcg
     5701 ctcgtcgtcc ggccctcttc gctcatcgtc aagtatcagg gcaagcagct tgacccgcgc
     5761 gaggtcgggc gcgagctgaa ggtctcggcc atcatggagg ccagcttcct gcgcgcgggc
     5821 gagcacctgc gcgtcaacgc gcagctcgtg gacgtgtaca ccggcgacat cctgtggagc
     5881 gaccgcatcg acgcctctgc gcaggacatc atcgcgctcc aagacctgat cgcgcagcgc
     5941 atcgccaagg gtctgaaggt cgagccggcg cccgccgaga aggccgaggc cgcggggcct
     6001 gcgacgcgca acgccgccgc ctacgaagag tacctgcgcg ggcgcgacta cttcgccaag
     6061 ttcctcttcg gcacgctcga ccccgaagag tgcgaccgcg ccatcgcgca cttccagcgc
     6121 gccatccagc tcgaccactc cttcgcgctc gcgcacagcg gtctcggcgc ctgcttcgcc
     6181 aaccgcgtct tgaagggtct gggcggcacg gaggactacg agctggcgga gaacgccttt
     6241 cagcgcgcgc tcgaactcga ccccgacgtg gtcgaggcg
//
LOCUS       DBNGOH010000881         3792 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2882343, whole genome shotgun sequence.
ACCESSION   DBNGOH010000881 DBNGOH010000000
VERSION     DBNGOH010000881.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3792)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3792)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3792
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2882343"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1848
                     /locus_tag="AC159P_17105"
     CDS             <1..1848
                     /locus_tag="AC159P_17105"
                     /inference="COORDINATES: protein motif:HMM:NF051698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor domain-containing
                     protein"
                     /protein_id="HIR3697215.1"
                     /translation="VFNQKVVNESRFQYERNRRRQEGEALAPTLRVNDAFIGGGAQVG
                     LSFNETDRFELQNYTSWAQGPHSLKAGARLRHLRLRDVSQQNFAGTFTFTTLEQYRQT
                     LLGLEDARPTQFSLSAGDPEARVSLTDFSPFIQDDWRVRPDLTLSAGLRYDRQTDIDS
                     GLNFAPRLAFAWAPRVGRGGGGNRQQQQTVIRGGFGVFYDRFNEFLPLQAARFDGVTQ
                     QQFLVTNATEQGRTILDLFPVVPSIATLEGFATSQTVRRLADDLRTPYTLQTALSVER
                     ALPYRTTLSVNFIAARTLHVLRTRNVNAPLTGEDGLPLRDAANNLVRPLSGQGNIFQY
                     ESSGVFNQQQLIISFNNRFGQRFSFSTSYTFNRARGDADGAFSSPVNQYDLTGEYGRS
                     QQDIRHRFALFGTVNAPWGVRLNPIVLANAGRPFNITLGRDVNADTLFNERPAFADAR
                     TPESDLRRTPLGDFDINPKPGQTVIPRNYGTGPSFFVVNLRAGKTFGFGPVAEARPPG
                     AGGGGRRGGGGGGGRGGRGGGGGGGRRGGGGFGGGGGEGGTERRYNLTLSVSVQNLLN
                     NTNEGVSVGNLRSPFFGQSVTTAGGFGRGGGSDASAGNRRVELQMRFGF"
     gene            2128..>3792
                     /locus_tag="AC159P_17110"
     CDS             2128..>3792
                     /locus_tag="AC159P_17110"
                     /inference="COORDINATES: protein motif:HMM:NF051698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR3697216.1"
                     /translation="MKVSHDKPRRPTFQCGAVARPLAALSFVLALACACATATAQDLN
                     GQLRGTVTDAQGAVVGGAAVTATHVETNTVYNATTNGEGAFVLPSVRLGRYTVAVEAG
                     GFRRTLVQDVDVEVGGIADLNVTLQVGSVEEQIEVSASATQEVINTTNAELGTVVDDR
                     RVQDLPLDGRNAAQLATLQAGVYFERSPDGQGDKLIVNGQRHRSLGVTLDGVDTQDNL
                     NRASSVMLDQPLLALAAENVQEFRVVTGIASAEFSRGGAQITAVTKSGTNELHGSVFE
                     FHRNTALNANEFFNNAATPGVPRPPLLRHQFGGRLGGPVFKDRTFFYFGYEQTRESKG
                     VPVNRTVYTAEARAGTFRYLTGLRNTPENVAANPSRVRSVNLLQCGAAVQTALNRFCV
                     DSRFNAANPASPDPFLRDNVFALIPLPNNFNIGDGLNTGGFRFNAPVKTLVHLPSFRL
                     DHRFNDKHSFAGTFNYNDRDIRGDFINDREPQFPGQRPLGTRLTHTRSFSAALTSAFT
                     PTIVQELRVGVVGGENAFLVTQPFDTPFALDLNTLTDPYDFENGDEV"
BASE COUNT          629 a         1495 c         1105 g          563 t
ORIGIN      
        1 gtcttcaacc agaaggtcgt caacgagtcg cgcttccagt acgagcgcaa ccgccgccgt
       61 caggagggcg aagcgctcgc ccccacgctg cgcgtcaacg acgccttcat cggcggcggc
      121 gcgcaggtcg gcctctcctt caacgagacc gaccgcttcg agcttcagaa ctacacctct
      181 tgggcgcaag ggccgcattc gctgaaggcg ggcgcacgcc tgcgccacct gcgtctgcgc
      241 gacgtgtcgc agcagaactt cgcgggcact ttcaccttca ccacgctcga acagtaccgg
      301 cagacgttgc tcggcctcga agatgcacgc cccacgcagt tctcactgtc cgcgggcgac
      361 cccgaagcgc gcgtctcgct gacagacttc agccccttca tacaggacga ctggcgcgtg
      421 cggcccgacc tcacgctctc cgcggggctg cgttatgacc ggcagaccga catcgactcg
      481 ggactgaact tcgcgccgcg cctcgccttc gcgtgggcgc cgcgcgtggg gcgcggcggc
      541 ggcggcaatc ggcagcaaca gcagaccgtc atccgcggcg gcttcggcgt cttctacgac
      601 cgcttcaacg agttccttcc gctgcaagcc gcgcgcttcg acggcgtcac acagcaacag
      661 ttcctcgtca ccaacgcgac cgagcagggc cgcaccatcc tcgacctctt ccccgtcgtc
      721 ccgtcaatcg ccacgctcga aggcttcgcc acctcgcaga ccgtccgccg actggccgac
      781 gatctgcgaa cgccttacac gttgcagacg gccctcagcg tcgagcgcgc gctgccctac
      841 cgcacgacgc tctcggtcaa cttcatcgcc gcgcgcacgc tccacgtcct gcgcacgcgc
      901 aacgtcaacg cgcctttgac gggcgaagac ggtctgccct tgcgagacgc cgcgaacaac
      961 ctcgtccgcc cgctctcagg gcaaggcaac atcttccagt acgaatcgtc cggcgtcttc
     1021 aaccagcagc agctcatcat cagcttcaac aaccgcttcg gccagcgctt ctccttctcg
     1081 acgagctaca ccttcaaccg cgcgcgcggc gacgccgacg gcgccttctc ctcgcccgtc
     1141 aaccagtacg atctgaccgg cgagtacggg cgctcgcagc aggacatccg ccaccgcttc
     1201 gcgctcttcg gcaccgtcaa cgcgccgtgg ggcgtgcgtc tgaacccgat agtgctcgcc
     1261 aacgcgggcc gccccttcaa catcacgctc gggcgcgacg tgaacgccga cacgctcttc
     1321 aacgagcgcc ccgccttcgc cgacgcgcga acgcccgaga gcgacctccg gcgcacgccc
     1381 ttgggcgact tcgacatcaa ccccaagccc ggccagaccg tcatccctcg caactacggc
     1441 accggcccct ccttcttcgt cgtcaacctc cgcgccggta agaccttcgg cttcggcccc
     1501 gtggccgaag cgcgcccgcc cggcgcaggc ggaggcggaa gacgcggcgg aggaggcggt
     1561 ggcgggcgcg gtggtcgcgg tggtggcggc ggtggcggtc gccgtggcgg cggcggcttc
     1621 ggcggcggcg gcggcgaagg cggcaccgag cggcgctaca acctgaccct ctccgtcagc
     1681 gtgcagaacc ttctcaacaa caccaacgag ggcgtctcgg tcggcaacct ccgctcgccc
     1741 ttcttcggcc agtcggtcac gaccgccgga ggcttcgggc gcggcggcgg cagcgacgcc
     1801 tccgccggca accgccgcgt cgaactgcaa atgcgcttcg gcttttaaga acagtaggca
     1861 gtaggctgcg ggcagcaggc agtaaaagca gaaagcagaa cacaagcgag cgaagggcgc
     1921 agaggctcgg gaggtaaagt cccgagcctc tgcgcccttc gctcttccgg cctctggcct
     1981 ctgcttctac tgctcactgc tcgccgctca ctgtcaaatt cttctcccct ctttcgccga
     2041 gttccgatat actccgcggc acccgagtta gtttccgcca ccccacgctt cggcccgaag
     2101 catacgccgt cagaaaggag ttcagccgtg aaggtttcac acgacaagcc ccgtcgcccg
     2161 acctttcagt gcggcgccgt cgcccgcccg ctggccgccc tgtccttcgt gctcgcgctc
     2221 gcgtgcgcct gcgccaccgc caccgcgcag gatttgaacg gccaactccg cggcaccgtc
     2281 acggacgcgc aaggggcggt cgtcggcggt gccgccgtga cggcgaccca cgtcgagacg
     2341 aacaccgtct acaacgcgac gacgaacggc gagggcgcct tcgtgctgcc gagcgtgcgc
     2401 ctcgggcgct acacggtcgc ggtcgaggcc gggggcttcc gccgcacgct cgtgcaggac
     2461 gtggacgtcg aggtgggcgg catcgccgac ctcaacgtca cgctccaggt cggctccgtc
     2521 gaggagcaga tcgaggtctc cgcctccgcc acgcaggagg tcatcaacac gacgaacgcc
     2581 gagctgggta cggtcgttga cgaccgccgc gtgcaagacc tgccgctcga cgggcgcaac
     2641 gccgcgcagc tcgccacgct ccaggccggc gtctacttcg agcgctcgcc ggacgggcag
     2701 ggcgacaagc tcatcgtcaa cggccagcgc caccgctcgc tcggcgtcac cctcgacggc
     2761 gtggacacgc aggacaacct caaccgcgcc tcctccgtca tgctcgacca gcctctgctc
     2821 gcgctcgccg ccgagaacgt gcaggagttc cgcgtcgtca caggcatcgc ctccgccgag
     2881 ttcagccgcg gcggcgcgca gatcaccgcc gtcaccaagt cgggcacgaa cgaactgcac
     2941 ggctccgtct tcgagttcca ccgcaacacg gccttgaacg ccaacgaatt cttcaacaac
     3001 gcggccacgc ccggcgtccc gcgcccgcca ctgctgcgcc accagttcgg cgggcggctc
     3061 ggcggccccg tcttcaaaga ccgcaccttc ttctacttcg gctacgagca gacgcgcgag
     3121 tccaaaggcg tccccgtcaa ccgcacggtc tacacggcgg aggcgcgcgc cggcaccttc
     3181 cgctacctca cggggctgcg caacacgccc gagaacgtcg ccgccaaccc ctcgcgcgtc
     3241 cgcagcgtca acctcctcca atgcggcgcc gcggtgcaga cggcgctcaa ccgcttctgc
     3301 gtggacagcc gcttcaacgc cgccaacccc gcctcgcccg accccttcct gcgcgacaac
     3361 gtcttcgcgc tcatcccgct gccgaacaac ttcaacatcg gcgacggcct caacaccggc
     3421 ggcttccgct tcaacgcgcc cgtcaagacg ctcgtccacc tcccctcgtt ccgcctcgac
     3481 caccgcttca acgacaagca ctcgttcgcc ggcaccttca actacaacga ccgcgacatc
     3541 cgcggcgact tcatcaacga ccgcgagccg cagtttccgg ggcagcgccc gctcggcacg
     3601 cgcctcacgc acacgcgctc gttcagcgcc gcgctgacct cggccttcac ccccaccatc
     3661 gtccaagagc tgcgcgtcgg cgtcgtcggc ggcgagaacg ccttcctcgt cacgcagccc
     3721 ttcgacacgc ccttcgccct cgacctcaac acgctcacag acccctacga cttcgagaac
     3781 ggcgacgagg tc
//
LOCUS       DBNGOH010000882         5420 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3249924, whole genome shotgun sequence.
ACCESSION   DBNGOH010000882 DBNGOH010000000
VERSION     DBNGOH010000882.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5420)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5420)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5420
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3249924"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1221
                     /locus_tag="AC159P_17115"
     CDS             <1..1221
                     /locus_tag="AC159P_17115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3697217.1"
                     /translation="DASRGRSAAWLDQLTVSYTGDMFANRALMLLAAAACLVVLHFRF
                     SRTERGGDEGGARRLTLLNLAPEAEAVHAGGETFDAGRDSLDHESFDRERSEDIRAAP
                     LSAPHIARKHAALPIVKTECVGARAGLRQLGAALSVECRLLREERGLVVLLPLAALLC
                     TLGVTYFGAEPVSSYSALYAIRTAEALLLFLFGVALFYTGEGLHRDRELRVEPVLWGF
                     PAPNYVFLLSKFLAVALLSLTLVALVGLSAVALQLIKGHGPLELRPYLVTYSVILLPT
                     ALFMVAAALALNVLVRDKYVAYAAGLGTGGGLFYLYGQGYKHWLYNPALYELWTYRDL
                     STAGDAQTLILTHRLYCLALTTLLLSLAHLCFARESTRGLRAHGRLTGRAWAALLALA
                     SATLALITGSLINV"
     gene            1286..1798
                     /locus_tag="AC159P_17120"
     CDS             1286..1798
                     /locus_tag="AC159P_17120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011605345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topology modulation protein"
                     /protein_id="HIR3697218.1"
                     /translation="MQKILVIGSGGAGKSTFARRLGARLRLEVIHLDAHHWRPNWVGT
                     PNDEWQEVVEELLRRDTWVMDGNYSGTLDTRLAACDTVIFLDLPRAVCLWRVLKRVIM
                     YRKRKRPDMAEGCGERFDLEFLRWVWGYRRRSRPKVVGLLREHERAKRVVWLRTSAEV
                     EVFLARAAGN"
     gene            1882..2349
                     /locus_tag="AC159P_17125"
     CDS             1882..2349
                     /locus_tag="AC159P_17125"
                     /inference="COORDINATES: protein motif:HMM:NF024375.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR3697219.1"
                     /translation="MTTHTTPAADETTIVHGDERPWIPVPGFAGVFIKVLVADEALKQ
                     VVFIFKFAPGTVLPRHKHLCHAIGYTVSGSWRYETGSLREGSLAYEPYASDHVPSSAE
                     GAEIVVFLKSENDLFLENEMPDGSTFIMDMNFFKLLHSLTAEQAAQFRIPGLG"
     gene            2431..2700
                     /locus_tag="AC159P_17130"
     CDS             2431..2700
                     /locus_tag="AC159P_17130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697220.1"
                     /translation="MSEEKTQYMPDERSFEERVFARFDAMDKRFDAVDERFDGVDRRL
                     DNLDARVQKLEEESERRAVETKPIWERALAEILKVQQALGEVKVV"
     gene            complement(2850..3593)
                     /gene="bshB1"
                     /locus_tag="AC159P_17135"
     CDS             complement(2850..3593)
                     /gene="bshB1"
                     /locus_tag="AC159P_17135"
                     /inference="COORDINATES: protein motif:HMM:TIGR04001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA];
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis deacetylase BshB1"
                     /protein_id="HIR3697221.1"
                     /translation="MNEVTRLDVLAVYSHPDDAELTAAGTLLKMKSLGYRTGVLDMTR
                     GEMGTRGTPERRAEEAREAARIMKLDVRLNLEQPDGHVWLTEESRRAMVRVIRTYGPK
                     LILTAHWDDPHPDHSNTAKIVREAARLASMRRYDEEAGQGAVAMPALAHSAYSRLVVP
                     SFIVDVSDFVEEKLDAIRAHASQFFDPHSTEPQTRISQEGFLKQLEYRMRYNGSLIGV
                     AAGEAFYVREALNVEDPVALLTRPMNIYS"
     gene            complement(3719..4264)
                     /locus_tag="AC159P_17140"
     CDS             complement(3719..4264)
                     /locus_tag="AC159P_17140"
                     /EC_number="3.1.4.58"
                     /inference="COORDINATES: protein motif:HMM:NF024952.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004113 - 2',3'-cyclic-nucleotide
                     3'-phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008664 - RNA 2',3'-cyclic
                     3'-phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HIR3697222.1"
                     /translation="MQSVVTLLDDQHYARVEAIWEELCQKFDVRGMYVTAYPHFSYQV
                     AEKYDEAACDEALRAVAARTAPFRVRTAGLGIFTVSNPILYVSIVRSPQLNGLHHDIW
                     DGIRQAAVPGAVSHYYQPEAWVPHITLAQGDIDQDKLAEIVRRLSARNFHWEITVNNL
                     TMIYDTGREQGLTCRYDFEKK"
     gene            4450..5226
                     /locus_tag="AC159P_17145"
     CDS             4450..5226
                     /locus_tag="AC159P_17145"
                     /inference="COORDINATES: protein motif:HMM:NF049367.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3697223.1"
                     /translation="MKLVVLGSGTSVPHPRRSSSAYWVEAAGRTLLLDPSAASVHRMP
                     QEGCDWAELDAVWVSHFHLDHVGGLAPFLFGTKYAPQTQKRTKPLHVYGGYGLKKLLE
                     KFDGAYDYGLFRQPFPLEVREVSPRAEFDVFDGLRAETFSTPHTRESLAVRLTETTGG
                     GASLVYTSDTGYTEALGRFARGADLFLMECSFFRSKPVETHLELADAMRLAQLSGARR
                     VVLAHLYPEWDGVDIAAEAKRLWDGETVAAEDGLRLEITK"
BASE COUNT          912 a         1827 c         1716 g          965 t
ORIGIN      
        1 gacgcctcgc gcgggcgcag cgccgcgtgg ctcgaccagc tcaccgtcag ctacaccggc
       61 gacatgttcg ccaaccgcgc gctcatgctg ctcgccgccg ccgcgtgtct ggtcgtcctc
      121 cacttccgct tctcaagaac cgagcgcggc ggggacgaag gcggcgcgcg gcggctgact
      181 ctgctcaacc tcgcgcccga agccgaagcc gtccacgcgg gcggcgagac tttcgacgcg
      241 ggccgcgaca gcctcgacca tgaaagcttc gaccgcgaac gctccgaaga tatccgcgcc
      301 gcccctctgt ctgccccgca catcgcgcga aaacacgccg cgcttccgat agttaaaacg
      361 gagtgcgtgg gcgcgcgggc cgggttgagg cagctcggcg cggccctctc ggtcgagtgt
      421 cgcctgctgc gcgaagagcg cggcctcgtc gtgctgctgc cgctggccgc gctcctctgc
      481 acgctgggcg tgacctactt cggggccgag ccggtgtcgt cttactccgc gctctacgcg
      541 atccgcacgg cggaggcgct gctgctcttc ctcttcggcg tcgccctctt ctacacgggc
      601 gagggcctgc accgcgaccg cgagctgcgc gtcgagccgg tgctgtgggg cttccctgcg
      661 ccgaactacg tcttcctgct ctcgaagttc ctcgccgtcg cgctgctctc gctcacgctc
      721 gtcgcgctcg tcggcctctc agccgtcgcg ctgcaactca tcaaaggcca cgggccgctt
      781 gagcttcgac cgtacctcgt cacctactcc gtgatcctgc tgccgaccgc cctcttcatg
      841 gtcgccgcgg ccctcgctct caacgtcctc gtccgcgaca agtacgtcgc ctacgcggcg
      901 ggcctcggca cgggcggcgg cctcttctac ctctacggtc agggctacaa acactggctc
      961 tacaacccgg ccctctacga actctggacg tacagagacc tctcgaccgc gggcgacgcg
     1021 cagactctca tcctcacgca cagactctac tgcctcgctc tcaccacgct cctcctctcg
     1081 ctcgcgcacc tctgcttcgc gcgcgagtca acgcgcggcc tccgcgcgca cgggcggctg
     1141 accggcaggg cttgggccgc cctcctcgcg ctcgcgtccg cgactctggc tctcatcacc
     1201 ggctctctca tcaacgtgtg agagaccgcc cgcgtgcgtc gcccgacttt ccaaagccat
     1261 ccgcggattt gttaacatcg gcggcgtgca gaagattctc gtcatcggct cgggcggggc
     1321 cggcaagtcc accttcgcgc ggcggctcgg cgcacgcctc cggctcgaag tcatccacct
     1381 cgacgcgcac cactggcgac cgaactgggt cgggacgccg aatgacgagt ggcaagaagt
     1441 ggtcgaagag ctgctgcggc gcgacacgtg ggtgatggac ggcaactact cgggcacgct
     1501 cgacacgcgg ctggcggcgt gcgacacggt gatcttcctc gacctgccgc gagccgtctg
     1561 cctctggcgc gtgctgaagc gggtgatcat gtaccgcaaa cggaagaggc cggacatggc
     1621 cgagggctgc ggcgaaaggt tcgacctcga attcctccgc tgggtgtggg gctaccgccg
     1681 ccgttcgagg ccgaaggtgg tcgggctgct cagggagcac gagcgggcga agcgggtcgt
     1741 ctggctgcgc acgtcggcgg aggtcgaagt atttctggcg cgtgccgcgg gcaactgacg
     1801 cgcgaatctg ttcacggcgc tcgcgccgta ttaaagtacg cctcgccatt tcagtgcgac
     1861 ccaccccgaa aggaaacccc tatgacgaca cacaccacgc ccgccgccga cgagacaacc
     1921 atcgtccacg gcgacgagcg cccatggatt cccgtccccg gcttcgcggg cgtcttcatc
     1981 aaagtcctcg tcgccgacga agcgctcaag caggtggtct tcatcttcaa gttcgcgccc
     2041 ggcacggtgc tcccgcgtca caagcacctc tgccacgcca tcggctacac cgtctccggc
     2101 tcgtggcgct acgagacggg gagcttgcgc gaaggcagcc tcgcctacga accctacgcc
     2161 agcgaccacg tcccttcgag cgccgaaggc gccgagatcg tcgtcttcct caagagcgaa
     2221 aacgacctct tcctcgaaaa cgagatgccc gacggcagca ccttcatcat ggacatgaac
     2281 ttcttcaagc tgctccactc gctcaccgcc gagcaggccg cccagttccg aatccccggc
     2341 ctcggctgag gactgcaaca tctgttgcat ccctattttt cgcttgaacg aatccaccac
     2401 cgcgcccata atcgcggctg tggattcgtt atgagcgaag aaaaaacaca gtacatgccc
     2461 gacgaacgct cttttgaaga gcgcgtcttc gcgcgcttcg acgcgatgga caagcgcttc
     2521 gacgccgtgg acgaaaggtt cgacggcgtt gaccgccgcc tcgacaacct cgacgcgcgt
     2581 gtgcagaaac tagaagaaga gtcggagcgg cgagccgtcg agacgaagcc gatttgggag
     2641 cgggcgctgg ctgagatttt gaaggttcag caggcgttgg gcgaagttaa ggtggtttga
     2701 aagacctgtc gaagaagatt gatgtcctca gcgttgataa tgttcaaatg cgggctacca
     2761 tgcgcgatca tgaactgcgg ctcgacaagc tagaggctga gccgacccgt tagagcgcaa
     2821 acgtcagttt acttccttac cttttcgcct cacgaataaa tgttcatcgg gcgcgtcagg
     2881 agcgcgacgg ggtcttcgac gttgagggct tcgcggacgt agaaggcttc gcccgcggcg
     2941 acgccgatga gcgagccgtt gtagcgcatg cggtattcga gctgcttgag gaagccctcc
     3001 tggctgatgc gcgtctgcgg ctcggtcgag tgcgggtcga agaactgcga ggcgtgggcg
     3061 cggatggcgt ccagcttctc ctcgacgaag tccgacacgt ccacgatgaa cgaggggacg
     3121 acgaggcgcg agtaggccga gtgcgcgagc gcgggcatcg cgacggcgcc ctgccccgcc
     3181 tcctcgtcgt agcggcgcat cgaggcgagg cgcgcggcct cgcggacgat cttcgcggtg
     3241 ttcgagtgat ccgggtgcgg gtcgtcccag tgcgcggtga ggatgagctt cgggccgtag
     3301 gtgcggatga cgcgcaccat cgcgcggcgc gactcctcgg tgagccagac gtggccgtcg
     3361 ggctgttcga ggttgaggcg cacgtcgagc ttcatgatgc gcgccgcctc gcgcgcctcc
     3421 tcggcccgcc gctcgggcgt cccgcgcgtc cccatctcgc cgcgcgtcat gtcgagcacg
     3481 cccgtccgat accccagaga cttcatcttc agcagcgtcc cggcggcggt cagctccgcg
     3541 tcgtccgggt gcgagtagac ggccagcacg tcgagtcggg tcacttcgtt cataatctta
     3601 aaagtaggca gcaggcagca ggcagcaggc agtcaaaacc tttttcagca aaatcatttc
     3661 agttaaagcg gcgacgtttg ccatctgtct tgactgcctg ctgcctgctg cccgctgcct
     3721 acttcttttc aaagtcgtaa cggcacgtca acccctgctc gcgcccggtg tcgtagatca
     3781 tggtcaggtt gttgacggtg atctcccagt ggaagttgcg tgcgctcaag cggcggacga
     3841 tctcggcgag cttgtcctgg tcgatgtcgc cctgcgcgag cgtgatgtgc ggcacccacg
     3901 cctccggctg gtagtagtgc gagacggcgc cggggactgc ggcctggcgg atgccgtccc
     3961 agatgtcgtg gtgcagcccg ttgagttgcg ggctgcggac gatggagacg tagaggatcg
     4021 ggttcgagac ggtgaagatg ccgaggcccg cggtgcggac gcggaaaggg gccgtgcgcg
     4081 cggcgacggc gcggagcgcc tcgtcgcacg cggcctcgtc gtacttctcc gccacctgat
     4141 aggagaagtg cgggtaggcc gtgacgtaca tgccgcgcac gtcgaacttc tggcacaact
     4201 cctcccagat cgcctcgacg cgcgcgtagt gctggtcgtc cagcagcgtg accacgctct
     4261 gcatcagtcg cgcgccctct ggccgaagga aactagggac atcacgcgta aagaattagc
     4321 ccaagccgcc aacgcggtca agcggcggcg gcgtcaatca accggttttt cacgccgcgg
     4381 gcgcggcgcg cgttgacgcc cgtcgctcgg ggttggtagc cttgcgggta aaggcgaaag
     4441 gaaagatcga tgaagctcgt cgtcctcggt tcgggcacgt ccgtgccgca cccgcggcgc
     4501 tcgtcctccg cttactgggt cgaggccgca ggccgcacgc tcctgctcga cccgagcgcc
     4561 gcttcggttc accgcatgcc gcaggaaggt tgcgactggg ccgaactcga cgccgtgtgg
     4621 gtgagccact tccacctcga ccacgtcggc ggcctcgcgc ccttcctctt cggcacgaag
     4681 tacgcgccgc agacgcagaa gcgcaccaag ccgctccacg tctacggcgg ctacggcctg
     4741 aagaagctgc tcgaaaagtt cgacggcgcg tacgactacg gcctcttccg acagccgttc
     4801 ccgctcgaag tccgcgaggt ctcgccgcgc gcggagttcg acgtcttcga cggcctgcgc
     4861 gcggagactt tctcgacgcc gcatacgcgc gagagcctcg ccgtcaggct caccgaaaca
     4921 acgggcggcg gcgcgtcgct cgtctacacc tccgacacgg gctacacgga ggcgctcggg
     4981 aggttcgcgc gcggtgccga cctcttcctg atggagtgct cgttcttccg ctcgaagccg
     5041 gtcgagacgc acctcgaact cgcggacgcg atgcggctcg cgcaactctc cggcgcgcgg
     5101 cgcgtcgtgc tcgcgcacct ctaccccgaa tgggacggcg tggacatcgc cgccgaggcg
     5161 aagaggctgt gggacggcga aacggtcgcg gcggaggacg ggctgcggct ggaaataacg
     5221 aaatgatgag cgcggaacga tgaatgatga atgagcggac aaactgaatg atgagaggca
     5281 cgctgctttt catcatcaac ttgatgacag gcacgctgtt tctcatcaat catcccgcgc
     5341 gcatcattta tcattcagtt ggacttttca ttcatcattc atcatttaac gctatgaaat
     5401 cccggctcat cgtcaacccc
//
LOCUS       DBNGOH010000883        14105 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3250053, whole genome shotgun sequence.
ACCESSION   DBNGOH010000883 DBNGOH010000000
VERSION     DBNGOH010000883.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 14105)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14105)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14105
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3250053"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3892)
                     /locus_tag="AC159P_17150"
     CDS             complement(<1..3892)
                     /locus_tag="AC159P_17150"
                     /inference="COORDINATES: protein motif:HMM:NF013867.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HIR3697224.1"
                     /translation="MSGISGSDDTFLTGEAGQHRGDDFPIVGMGASAGGITAFKEFFE
                     RVPADSGMAYVVILHLSPEHDSKLAEVLQASAAIPVAQVVESIKIEPNHVYVIPPNKS
                     LSMDDGVLTLSEIKGYEERRAPVDIFFRTLATEHTTRAVAVILSGTGANGSMGVKRVK
                     EMGGIILVQDPREAEYSDMPRNSMATGLVDYVLPVAEIPARIIAYKENLEEVRITAEV
                     PERPETDEQALRDIFTQLRVRTRHDFSNYKRATVMRRLQRRINVHDLTGLPDYARFMR
                     EHNDEAQALLKDLLISVTNFFRDPEAFRSVEQKIIPQLFEGKGADDYVRVWVAGCATG
                     EEAYSLTMLLLDYAAKLTAPPSIQVFATDIDEQAVSIARDGFYTDTDVADVPPERLRR
                     YFTKETDGYRVRREMRETVLFASHNLIKDPPFSHLDMASCRNLLIYLNRTAQERVMQV
                     IHFALNPGGFLFLGTSESVEGAGDLFVTVDKDAHVYQGRVVSPRMALPLPDMSPSVPT
                     GQRARVEHTSEPRAREERLTYSDLHHRLIEQYGAPSVVVNEEYTIVHLSERAGRYLQF
                     AGGDASLNLLKVIRPELRLELRTALYQAAQNRTNVEARGIVARLDDHTQTVNLMVRPV
                     LREDDTARGFFLVLFEESPADADAESAPAVAEVVTPVEPAMLHLEEELLRVKAQLRSN
                     VEQYETQAEEMKASNEELQAMNEELRSSTEELETSKEELQSLNEELHTVNQELKVKIE
                     ELSHANNDFRNLMFSTDIGTVFLDRSLHIKLFTPRARDIFKLIPADVGRPLSDISSNF
                     PHDGLLADIETVLDRLQSVEREVGTRDGRFYLMRVVPYRTAEDRIEGVVLNFVDITQL
                     RRAEEARFFLASIVESSQDSIITVNFDGIITSWNRAAETLYGYAAAEAMGKPLTMLTL
                     PEDLKEVLANADKVKHSETVEIYDTIRVNKDGRQMNLEVMLSPVRDAAGRVVGVSTIA
                     RDVTDRRRAEEALRESEERLRLLVESVKDYAILTLDAERRVTSWNTGAERIFGYAESE
                     IIGQSGDILFTPEDREQGIPEKEAATARVAGRAADERWHIRKDGMPFYASGVLVPLSG
                     GGRQGFAKIARDLTEQKRAEEELRHAHDALEGRVHERTAELIDLNETLLTEVKERTAA
                     EEHARQLMRQIVTAQEDERRSVARDLHDHLGQQLTGLRIKLENHKQSCGNDPSHRAEV
                     EQIQAIAERLDADVDFLSWELRPASLDELGLAAALANFVQEWSEHFDIPAEFHTMGLD
                     RQRLPPEIEINLYRIAQEALNNVSKHAGAKGVGVL"
     gene            4181..5188
                     /locus_tag="AC159P_17155"
     CDS             4181..5188
                     /locus_tag="AC159P_17155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012598585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HIR3697225.1"
                     /translation="MPTEQIVCVGASSGGIEALRTLVAGLPADFGAAILVVLHTSPES
                     PGILGSILDRAGALPAANAVDGERIQPGRIYVAPSDYHLLVEPGIVRLTRGPKENRFR
                     PAIDPLFRSAASVYGPVAVGVILTGNLDDGTAGLRAIKQLGGTTIVQDPEEALFSSMP
                     RSALEHVPVDYCLPVAEIATMLTRLASARVTDEEVCEVPEEMDIENRIAGEDNAIEAG
                     VKKLGDPSSYACPECHGVLLQLKGENPLRFRCHTGHAYSVQSLLAEVTEKMEEALWSA
                     IRSFEEGAMLLHQMAGHPHEGAKEDTTEELLHRARETKRRAELLKQAVTGNGKPSAAS
                     D"
     gene            complement(5210..7441)
                     /locus_tag="AC159P_17160"
     CDS             complement(5210..7441)
                     /locus_tag="AC159P_17160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002625717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HIR3697226.1"
                     /translation="MTFKQPSPADVPELWAGVECTVNRVGDRYFDQLERSGHARRGED
                     LDLFAALGARAVRYPVLWERTAPDGLATADWSWADARLARLRELRLRPIVGLVHHGSG
                     PRETSLVDDDFAAKLAAYARAVAERYPWVGDYTPVNEPLTTARFGCLYGHWHPHARDA
                     LSFARAVLNQCRAVVLSMRAVREVNSSARLVQTEDLGQTYSTRLLTYQADFENERRWL
                     TWDLLAGRVVRGHAMWDYLVWLGVAEGELEWFAENPCPPDIVGVNHYVTSERLLDERL
                     SRYPASTHGGNGRHVYADVEAVRARADGVAGSRSLLRAAWERYRLPVAATEAHLGCTR
                     EEQMRWLKEVWDGACDLRREGADVRAVTAWSVLGAYDWDSLLTESRGRYEPGVFDLRA
                     PAPRPTALARMLKELAAGREHDHPVLDSPGWWRRLERLWHPVHTGATRARAVRSAGVK
                     GGRPLLIVGKTGTLGQAFARACDARGLSYVTTTRGEMDIADAASVAAALGRAEPWAVV
                     NAAGYVRVDDAEREREACRRANAHGPATLAAACAARGIQLLTFSSDLVFDGAKREPYV
                     ERDAPAPLCEYGRSKAEAEALVLKALPAALVVRTSAFFGPWDEYNFVTVLLRALAEGV
                     EFAAADDVVVSPTYVPDLTHACLDLLTDGEGGVWHLSNAGALTWADFARLAAERAGLE
                     SSLVRGCPSASLGLAAPRPAFSALDSERGALLPPLEDALARYFNECELWAPSRRRVEV
                     RTG"
     gene            complement(7459..8682)
                     /gene="glf"
                     /locus_tag="AC159P_17165"
     CDS             complement(7459..8682)
                     /gene="glf"
                     /locus_tag="AC159P_17165"
                     /EC_number="5.4.99.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008767 - UDP-galactopyranose mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009273 - peptidoglycan-based cell wall
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
                     /protein_id="HIR3697227.1"
                     /translation="MFDYLIVGAGFAGSVLAERLANGSNKKVLICDRRPHIGGNAYDH
                     YNEDGLLVHKYGPHIFHTNSREVFDYLSRFTEWRQYEHRVLASVDGQLVPIPINLDTI
                     NKLYGLSLTSFEVEEFFRKVAEPVEQVRTSEDVVVSKVGRELYEKFFRNYTRKQWGLD
                     PSELDASVTSRVPTRTNRDDRYFTDTYQAMPLHGYTRMFEKMLDHPNIKIMLNCDYRE
                     LAGEIDYREMIYTGPVDAFFDYRYGKLPYRSLEFKHETHNAAQFQPAPVVNYPNEHAH
                     TRVTEFKDLTGQEHSKTSVVYEFPRAEGDPYYPVPRRENAEVYAKYKALADATSGVHF
                     VGRLATYKYYNMDQIVAQALTVYGKLRGEKRAVAAVAQAGRAASNGNNTGKVNGNGNG
                     NARLPSNVTALTGGR"
     gene            complement(8871..10118)
                     /locus_tag="AC159P_17170"
     CDS             complement(8871..10118)
                     /locus_tag="AC159P_17170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018479181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HIR3697228.1"
                     /translation="MRAEMKIAEDRSTPLSQLVSKTPDLVCLSHLRWDFVYQRPQHLL
                     SRFAGGRRVFFVEEPIITDEAEARLEVSRREGGLHVVVPHVPRDLAGEGKLDIAQRSL
                     LDRLFAEHGIDAHVLWYYTPMAVRWSRHLKPLAVVYDCMDELSAFKGAPRSMKEAEAE
                     LFARADMVFTGGQSLYEAKREQHANAYCFPSSIDAPHFAQARCVSRDPEDQQAIPRPR
                     VGYFGVIDERLDIELLDRVAALRPDWHLVMVGPVVKIDPAELPRRANIHYLGGKSYKE
                     LPAYIAGWDVAVMPFARNDSTRFISPTKTPEYLAAGRPVVSTSIRDVVRPYGEMGLVH
                     IADTPDEFVAAIEAALREDADGRRRAADEFLSQTSWDRTWGRMAELIEDAVGARRASL
                     AAGQTMTKAAAQGASAVSYASGD"
     gene            complement(10444..10794)
                     /locus_tag="AC159P_17175"
     CDS             complement(10444..10794)
                     /locus_tag="AC159P_17175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697229.1"
                     /translation="MENVKASGFPFGLLELDGAGMVIRFTPAHGEGAANGQPREVVGH
                     DFFEDIAPVEELRRLKGRFLAFMAFGDSAQRLDFRFTYKDLVVAAHIVLTRLTDGPEL
                     GGTRLALVRLTPGA"
     gene            complement(11284..13137)
                     /locus_tag="AC159P_17180"
     CDS             complement(11284..13137)
                     /locus_tag="AC159P_17180"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014851.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 protein"
                     /protein_id="HIR3697230.1"
                     /translation="METPRPEYPRPQFVRAEWLNLNGEWEFAFDDANKGRSLGWQYGL
                     ALEGRIVVPFPYQSSRSGIDDNSVHEVVWYARTFEVPAEWRGRDVLLHFGAVDYRTTL
                     WVNGQEVGHNQGGHVPFSFDIAPYLKSNEPNRLTVRVEDRQNPRQPRGKQSATGLPHD
                     IDYYCTTGIWQTVWLEPVPPVRIGEIKLTPSARTSSLDIMVYLHAPSVERRLEAEAFE
                     LDTRAVVARAEEETTAATCQLDLHIPEARLWSPDSPHLYGLRLRLFEGGELIDEVESY
                     AGLRDVELRGGRVRLNGEPVYLAMILDQGYWPESYLAAPSDEALRADVLWVKRFGFNG
                     ARKHQKIEDPRWLYWCDRLGLLVWEEMPNAREWSSQAEERLAAEWKRAVQRDYNHPCI
                     VTWVPVNESMGFPGLRKQHPGQYAFIERMVAMTRQLDPRRPVIDNDGWEHTDITDIVA
                     IHDYTPTAELLRERYRETVGGSTLPAHVWIGNKPLFVLGSRYRGQPVMLTEVGGFLMI
                     PPDVPPERRDMLYRFYGSAGTYEDLLAKYRDLMEGIASLPFIAGFCYTQLTDIEQEIN
                     GLLTYDRQPKVAPERVAEIHRRLFFDPQTGQPPDETAHGTPQPESDCLTPS"
     gene            13441..13890
                     /locus_tag="AC159P_17185"
     CDS             13441..13890
                     /locus_tag="AC159P_17185"
                     /inference="COORDINATES: protein motif:HMM:NF013936.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HIR3697231.1"
                     /translation="MNAFDLLMMDHQKVSGIFDRLEMNDGGVAGSRAELFRQLKNELD
                     VHAHVEETIFYPALKEAEETRELIAEAYNEHAAVKDLLAEMEATAPGEGGWDDLLAEL
                     RENVEHHVEEEEGDLFEQARLVLTSEQAEALGARMEREKQRRMTATA"
BASE COUNT         2314 a         4574 c         4735 g         2482 t
ORIGIN      
        1 ggaggacgcc gacgcctttg gccccggcgt gcttcgagac gttattcaac gcctcctgcg
       61 cgatgcggta gaggttgatc tcgatctccg gcgggaggcg ctgcctgtcc agacccatgg
      121 tgtgaaactc ggcggggatg tcgaagtgtt cggaccactc ctggacgaag ttggcgagcg
      181 ccgccgcgag gccgagttcg tcgagcgatg cgggccgcag ctcccacgag aggaagtcca
      241 cgtccgcgtc gagccgctcg gctatcgcct gtatctgctc gacctcggcc cggtgcgagg
      301 gatcgttgcc gcaggactgc ttatgatttt ccagcttgat gcgaagtccg gtgagttgct
      361 ggccgaggtg gtcgtggagg tcgcgcgcga cgctgcggcg ttcgtcctcc tgcgccgtga
      421 cgatttgccg catgagttgc cgcgcgtgct cctcggctgc cgttcgctcc ttgacctcag
      481 tcagcagggt ttcgttcaag tcgattaact cggcggtgcg ctcgtgcacg cgcccctcca
      541 gtgcgtcgtg cgcgtgccgc aactcttctt ccgcgcgttt ttgctcggtc aggtcgcgcg
      601 cgatcttggc gaagccctgc cgaccgccgc cgctcaaagg cacaagcacg ccggaggcgt
      661 agaagggcat gccgtccttg cggatgtgcc agcgctcgtc cgccgcgcgc ccggcgacgc
      721 gggctgtggc tgcttccttt tccgggatgc cctgctcgcg gtcttcgggc gtgaagagga
      781 tgtctcccga ttgaccgatg atctcgcttt ccgcatagcc gaagatccgt tccgccccgg
      841 tgttccaact cgtgacgcgc cgctcggcgt cgagcgttaa gatggcgtag tccttcacgc
      901 tctcgacgag caggcgcagc cgctcctcgc tttcgcgcag cgcctcttcc gcccgcctgc
      961 ggtcggtcac gtcgcgcgcg atggtcgaaa cgccgacgac ccttcccgcc gcgtccctga
     1021 cgggcgataa catcacttcg aggttcatct gccgcccgtc cttgttcacc ctgatggtgt
     1081 catagatttc caccgtctcg ctgtgcttga ccttgtcggc gttggcgagc acctctttca
     1141 ggtcttccgg gagcgtcagc atcgtcaatg gcttgcccat ggcttccgcc gccgcatagc
     1201 cgtagagcgt ttccgccgcc ctattccaac tcgtgatgat tccgtcgaaa ttaaccgtga
     1261 tgatggaatc ctgcgacgac tccacaatcg aagccagaaa gaatctggct tcttccgcgc
     1321 gcctgagctg ggtgatgtca acgaagttca gcacgacgcc ctcgatgcgg tcttccgccg
     1381 tgcggtaagg caccacccgc atcaggtaga agcgcccgtc gcgcgtcccg acttcgcgct
     1441 cgaccgattg gagccggtcg agcaccgttt ctatgtcggc gagcagcccg tcgtggggaa
     1501 agttgctcga gatgtccgag agcgggcggc caacgtccgc ggggatgagt ttgaagatgt
     1561 cgcgggcgcg cggcgtaaag agcttgatgt ggagcgagcg gtcgaggaag acggtgccga
     1621 tgtcggtcga gaacatcagg ttgcggaagt cgttgttggc gtggctcagc tcctcgatct
     1681 tgaccttcaa ctcctgattg accgtgtgca gctcctcgtt cagggactgc aactcttcct
     1741 tcgaggtctc cagctcttcg gtcgaggagc gcagctcctc gttcatcgcc tgaagctctt
     1801 cgttcgaggc tttcatctcc tcggcctgcg tctcgtactg ctcgacgttg gagcgcaact
     1861 gcgccttgac gcgcagcagc tcctcttcga ggtggagcat cgccggctcg acgggggtga
     1921 cgacctccgc caccgccggg gcgctttcgg cgtccgcgtc cgcgggagac tcctcgaaca
     1981 gcacgaggaa gaagccgcgc gccgtgtcgt cctcgcgcag cacggggcgc accatcaggt
     2041 tgactgtctg cgtgtggtcg tcaaggcgcg ccacgatgcc gcgcgcctcg acgttcgtgc
     2101 ggttctgagc ggcctgatag agcgccgtgc gcagctccag ccgcagctcg gggcggatga
     2161 ccttgagcag gttgagcgag gcatcgcccc cggcgaattg caggtagcgg cccgcgcgct
     2221 ccgacaggtg aacgatggtg tactcttcgt tgacgacgac cgaaggcgcc ccgtactgct
     2281 cgatcaggcg gtggtgcagg tcggagtagg tgagtcgttc ttcacgcgct ctcggttcgc
     2341 tagtgtgctc gactcgtgct cgttggccgg tgggcacgct cggcgacata tcgggcaggg
     2401 ggagcgccat gcgcgggctg acgacgcggc cctgatagac gtgcgcgtcc ttatcgaccg
     2461 tgacgaagag gtcgcccgcc ccctcaacgg attccgaggt gccgaggaag aggaatccgc
     2521 cggggttgag ggcgaaatgt atgacctgca tgacgcgctc ctgcgccgtg cggttgaggt
     2581 agatcaggag gttgcggcag gaggccatgt cgaggtgcga gaagggtggg tctttgatga
     2641 gattgtgaga tgcgaagagc accgtctcgc gcatctcgcg cctgacgcgg tagccgtccg
     2701 tctccttcgt gaagtagcgg cgcaggcgtt cgggcggcac gtccgccacg tccgtgtcgg
     2761 tgtaaaagcc gtcgcgcgcg atcgagacgg cctgttcgtc gatgtccgtg gcgaagacct
     2821 gtatgctcgg cggggctgtc agcttcgccg cgtagtccag gagcaacatc gtcaacgagt
     2881 aggcctcttc gcccgtcgca cagccggcca cccagacccg cacgtaatcg tccgcgcctt
     2941 tgccctcgaa gagttgcggg atgatcttct gctcgaccga ccggaacgct tcggggtcgc
     3001 ggaagaagtt cgtcacgctg atgagtaaat ccttcaagag cgcctgcgcc tcatcattgt
     3061 gctcgcgcat gaagcgcgcg tagtcgggca ggcccgtgag gtcgtggacg ttgatgcggc
     3121 gctggagtcg gcgcatcacg gtcgcgcgtt tgtagttcga gaagtcgtgg cgggtgcgca
     3181 cgcgcaactg cgtgaagatg tcgcgcagcg cctgctcgtc cgtctccggt cgctccggca
     3241 cttcggcggt gatgcgaacc tcttcgaggt tctctttgta agcgatgatc ctggccggta
     3301 tctcggcgac ggggagcacg tagtccacca gcccggtcgc catcgagttg cgcggcatgt
     3361 ccgagtactc ggcctcgcgc gggtcttgca cgaggatgat gccgcccatc tccttgacgc
     3421 gcttgacgcc catcgagccg ttcgcgcccg tgcccgagag gatgaccgcg acggcgcgcg
     3481 tcgtgtgctc cgtggcgagc gtgcggaaga agatgtcaac cggcgcgcgc cgctcctcgt
     3541 agcccttgat ctcggagagc gtgaggacac cgtcgtccat cgagaggctt ttgttcgggg
     3601 ggatgacgta gacgtggttc ggctcgatct tgatcgactc gaccacctgc gcgacgggta
     3661 tcgcggcgga ggcttgaagc acctcggcca gcttgctgtc gtgctcgggc gagaggtgca
     3721 ggatgactac gtaggccatg ccgctgtcgg cggggacgcg ctcgaagaac tccttgaacg
     3781 ccgtgatccc tccggctgag gcccccatgc cgacgatcgg aaaatcatcg cccctgtgct
     3841 gtccggcctc tccggtgagg aacgtgtcat cactgccaga aattccgctc atgccgcaac
     3901 tcccctaaag attacgaagc gctcaggctg gatggcacgc cctcggaaac ctgacacctg
     3961 tccttcataa ttttaatact tctttgttcg cggggtggcc ggggcgttac aagcaaagtc
     4021 taatccaacc cttcatttag tgtatagcga aaaacgggac gacaagcccc gccgcctccg
     4081 aagtgtggta taaagacatc agaattcatc attcccctgc accggcttat atcgatccct
     4141 gacgccgcgc tccacgcgcc gggcgagaaa gaggcttcaa ttgccgacag aacagatcgt
     4201 ctgtgtaggg gcttcctcgg gcggcatcga ggcgctcagg acgttggtgg ccggactccc
     4261 tgccgacttc ggtgcggcca tcctcgtcgt gctccacacc tcgcccgaat ctccgggaat
     4321 cctgggcagc atactcgacc gcgccggagc gcttccggcg gctaacgccg tggacggtga
     4381 gcgcatccag ccgggtcgca tttatgtcgc gccgtccgac taccatctgc tcgtcgaacc
     4441 cggcatcgtc cgcctgacgc gcgggccaaa ggagaaccgt tttcgcccgg ccatcgaccc
     4501 gctcttccgc tcggcggcct ccgtctacgg ccccgtggcc gtgggcgtca tcctgaccgg
     4561 caacctggac gacggcaccg ccggactgcg tgccatcaag caactcggcg gcaccaccat
     4621 cgttcaagac cccgaagaag ccctcttctc ttcgatgcct cgaagcgcgt tggaacacgt
     4681 gcccgtggat tactgcctac ccgttgcgga gattgcaact atgttgacgc gactggcgag
     4741 cgcgcgtgtc acggatgaag aagtgtgtga ggtgccagag gagatggaca tcgaaaacag
     4801 aatcgccggg gaagataacg ccatcgaggc cggcgtgaaa aagctcggag acccctcttc
     4861 ttacgcctgc ccggaatgtc acggcgtgct cctgcaatta aagggcgaga accctctccg
     4921 cttccgctgc cacacgggcc acgcctattc ggtgcaaagc ctcctggcgg aagttaccga
     4981 aaagatggag gaggcgctct ggagcgccat ccgcagcttc gaagaaggcg cgatgctgtt
     5041 gcaccagatg gccggccatc cgcacgaggg cgcgaaggag gacaccaccg aagaactcct
     5101 ccaccgggcg cgcgaaacca agcgccgcgc cgaactgttg aaacaggcgg tgacgggtaa
     5161 cggaaagccg agtgccgcct cggattaaat cagtcgctcc gccatgctct catcccgtcc
     5221 gcacctccac acgccgccgg ctcggcgccc atagttcgca ctcgttgaag taacgtgcga
     5281 gcgcgtcctc gagcggcggg agcagtgcgc cgcgctcgct gtcgagcgcg ctgaaggccg
     5341 gtcgcggggc cgcgaggccg agcgaggccg agggacaccc gcgcacgagc gaactctcca
     5401 ggcccgcgcg ctcggcggcg agccgcgcga agtcggccca cgtcagcgcg cccgcgttcg
     5461 agaggtgcca cacgccgccc tcgccgtcgg tgagcaggtc gaggcaggcg tgcgtgaggt
     5521 cgggcacgta agtcggcgag acgaccacgt cgtctgccgc cgcgaactcg accccttcgg
     5581 cgagggcgcg caagagcacg gtcacgaaat tatattcgtc ccacgggccg aagaacgcgc
     5641 tcgtgcggac gacaagcgcg gcgggcaatg ccttgagcac gagcgcctcc gcctcggcct
     5701 tgctccgacc gtattcgcag agcggtgcgg gtgcgtcacg ctcgacgtaa ggctcgcgct
     5761 tcgcgccgtc gaacacgagg tcggaagaga aggtgagcaa ttgaatgccg cgcgcggcac
     5821 aggctgcggc cagcgtcgcg gggccgtgcg cgttcgcgcg gcggcacgct tcgcgctcgc
     5881 gctcggcgtc gtccacgcgg acgtagcccg cagcgttcac caccgcccac ggctctgcgc
     5941 gcccgagcgc cgccgcgacc gacgccgcat cggcgatgtc catctcgccg cgcgtggtgg
     6001 tgacgtaaga gagtccgcgc gcatcgcacg cgcgcgcgaa cgcctgcccg agcgtgcctg
     6061 tcttgccgac tatcaaaagc gggcgcccgc ccttcacgcc ggcgctgcga accgcgcgcg
     6121 cgcgcgtcgc gcccgtgtgc accgggtgcc agaggcgttc gagcctgcgc caccaccccg
     6181 gcgagtcgag cacagggtgg tcgtgctcgc gccccgccgc gagttccttg agcatccgtg
     6241 cgagcgccgt cggtcgcggc gcgggggcgc gcaggtcgaa gacccccggt tcgtagcggc
     6301 cacggctctc tgtcagcaga ctgtcccaat cgtacgcgcc gagcacggac cacgcggtca
     6361 cggcgcgcac gtctgcccct tcgcgccgga ggtcgcacgc gccgtcccac acctctttga
     6421 gccagcgcat ctgctcctcg cgcgtgcagc cgaggtgcgc ttccgtcgcg gcgacgggca
     6481 ggcggtaacg ctcccacgcc gcgcgcaaga gcgagcgcga cccggccacg ccgtcggcgc
     6541 gcgcgcgcac cgcttccacg tcggcgtaca cgtgtcgccc gttgccgccg tgcgtcgagg
     6601 ccgggtagcg cgacagccgc tcgtcgagga ggcgctcgct cgtcacgtag tggttgacgc
     6661 cgacgatatc cggcgggcag gggttctcgg cgaaccactc aagctcgcct tcggcgacgc
     6721 cgagccacac gagatagtcc cacatcgcgt ggccgcgcac gacgcggccc gcgagcaagt
     6781 cccacgtcag ccaacgtcgc tcgttctcga agtcggcctg ataagtgagc aggcgcgtcg
     6841 agtaggtctg gccgaggtct tcggtctgca ccagccgcgc cgaactgttg acctcgcgca
     6901 ccgcgcgcat cgagaggacg acggcgcggc actggttgag cacggcgcgc gcgaacgaga
     6961 gcgcgtcgcg cgcgtgcggg tgccagtggc cgtagaggca accgaagcgc gccgtggtca
     7021 gcggctcgtt gaccggcgtg tagtctccga cccacgggta gcgctcggcc acggcgcgcg
     7081 cgtaagccgc aagcttcgcg gcgaagtcgt catcgacgag gctcgtctcg cgcgggccgc
     7141 tgccgtggtg gacgaggccg acgatgggtc gcaggcgcag ctcgcgcagg cgcgcgagcc
     7201 gcgcgtcggc ccatgaccaa tcggctgttg cgagtccgtc gggggcggtg cgctcccaga
     7261 ggacggggta gcggacggcg cgcgcgccga gcgcggcgaa gaggtcgagg tcttcgccgc
     7321 gccgcgcgtg gccgctccgc tccagctggt cgaagtagcg gtcgcccacg cggttgaccg
     7381 tgcactcgac gccggcccac aactcgggaa cgtcggcggg tgaaggctgc ttgaaggtca
     7441 tcccggcggc ccctctcctc acctgccgcc cgtcagggcc gtcacgttcg acggcagccg
     7501 cgcgttgccg ttcccgttgc cgttcacctt acctgtgttg ttaccgttgc tcgccgcgcg
     7561 tccggcctgc gccacggcgg ccaccgcgcg cttctcgccg cgcagcttgc cgtagaccgt
     7621 cagcgcctgc gccacgatct gatccatgtt gtaatacttg tacgtggcga gccgcccgac
     7681 gaagtggacg cccgacgtgg cgtccgcgag cgccttgtac ttcgcgtaga cttcggcgtt
     7741 ctccctgcgc ggcacggggt agtaggggtc gccttcggcg cgcgggaatt cgtagacgac
     7801 gctcgtcttc gagtgctcct gaccggtcag gtccttgaac tcggtgacgc gcgtgtgggc
     7861 gtgctcgttc gggtagttga cgacgggcgc gggctggaac tgcgcggcgt tgtgcgtctc
     7921 gtgcttgaat tcgagtgagc ggtacgggag cttgccatag cggtaatcga agaacgcgtc
     7981 caccggcccc gtgtagatca tctcgcggta gtcgatttcg cccgcgagct cgcggtagtc
     8041 acagttgagc atgatcttga tgttcgggtg gtcgagcatc ttctcgaaca tgcgcgtata
     8101 gccgtggagc ggcatcgcct gataggtgtc cgtgaagtag cggtcgtcgc ggttcgtgcg
     8161 cgtcggcacg cgcgaggtga cggaggcatc aagctccgac gggtcgaggc cccactgctt
     8221 gcgcgtgtag ttgcgaaaga acttctcgta cagctcgcga cccaccttcg agacgaccac
     8281 gtcttcgctg gtgcgcacct gctcgaccgg ctcggccacc ttcctgaaga actcctcgac
     8341 ctcgaaggag gtgagcgaga ggccgtagag cttgttgatg gtgtcgaggt tgatcgggat
     8401 ggggacgagc tgcccgtcca ccgaggcgag cacgcggtgc tcgtactgcc gccactcggt
     8461 gaagcgcgag aggtagtcga agacctcgcg cgagttggtg tggaaaatgt gggggccgta
     8521 cttgtggacg aggaggccgt cctcgttgta atggtcgtaa gcgttgccgc cgatgtgcgg
     8581 acgccggtcg cagatcaaga ccttcttgtt cgacccgttc gccagccgct cggccaaaac
     8641 gctcccggcg aaaccggccc ctacaatcag ataatcaaac atcgatttaa tctctccccc
     8701 tggttcgtta acgtgaagtc accaccgctt gaattattaa gcgtcggtca tgactggaga
     8761 ggcagggctt gtccctgccc gccgcagtcc cattgtcatt caacagcgaa tcggtcgaaa
     8821 ctcatgggcc gcggacaagc ctgcccctac aatgctgcgg cgcgtcttca tcaatcgccg
     8881 ctcgcgtagc tcacggcgga cgcgccctgc gccgccgcct tcgtcatggt ctgccccgcg
     8941 gcgagtgagg cgcggcgcgc gccgaccgca tcttcgatca actcggccat gcgcccccac
     9001 gtgcgatccc acgaagtctg cgagaggaat tcgtcggcgg cgcgccggcg cccgtcagcg
     9061 tcctcgcgga gcgccgcctc gatggccgcg acgaactcgt cgggcgtgtc ggcgatgtgc
     9121 accaagccca tctcgccgta ggggcgcacc acgtcgcgga tcgaggtcga gactacgggc
     9181 cgaccggcgg cgaggtactc gggcgtcttc gtcgggctga tgaagcgcgt cgagtcgttg
     9241 cgcgcgaagg gcatcacggc cacgtcccag ccggcgatgt aggcgggcag ctctttgtaa
     9301 gacttgccgc cgaggtagtg gatgttcgcg cggcgcggca gctccgccgg gtcgatcttg
     9361 acgacagggc cgaccatgac gagatgccag tcgggccgca gggccgcgac ccgatcgagc
     9421 agctcgatgt ccagccgctc gtcgatgacg ccgaagtatc cgacgcgagg gcgcgggatg
     9481 gcttgctggt cttcggggtc gcgcgagacg cagcgcgcct gcgcgaagtg gggcgcgtcg
     9541 atgctcgaag ggaagcagta ggcgttcgcg tgctgctcgc gcttggcctc gtagaggctc
     9601 tgcccgccgg tgaagaccat gtcggcgcgc gcgaacagtt cggcctccgc ttccttcatc
     9661 gagcgcggcg cacccttgaa ggccgacagc tcgtccatgc agtcgtagac gaccgcgagc
     9721 ggcttgagat ggcgcgacca gcggacggcc atcggcgtgt agtaccagag gacgtgagcg
     9781 tcgatgccgt gctcggcgaa gagccggtcg agcagcgagc gctgcgcgat gtcgagcttc
     9841 ccttcgcccg cgaggtcgcg cggcacgtgc ggaacgacga cgtgcaagcc cccttcgcgg
     9901 cggctgactt cgaggcgggc ttccgcctcg tccgtgatga tcggctcctc gacgaagaag
     9961 acgcggcgcc cgcccgcgaa gcggctcagg aggtgttgcg gcctctggta gacgaagtcc
    10021 cagcgcaggt gcgacaggca gacgaggtcc ggcgttttgg atacaagctg cgataagggt
    10081 gtggaacgat cctccgcgat tttcatttcg gcgcgcatag ttctctccaa agcggggaca
    10141 ccttccccgt aagttttagc tccggtgaag cgaaaagtct gttaatggtg cgggcgactc
    10201 gggaggcaca tccgcactta cagttaggcg gggattcagg tgtgcgaaga caccgacctc
    10261 cgaggaacgc accgcgggca cacggtctcg actctctgac acaagggggg aatcatgaaa
    10321 atagcctata cccacgcgcc ctgtcggtac gcgcgcgtac tcattacttt cgtgatgatt
    10381 acgtcgatga attgtcacac gccggtcgag gcgcgcggtc tcttttggag cgagggaagc
    10441 gttctacgcg ccgggcgtga ggcggacgag ggcgaggcgc gtgccgccga gttcggggcc
    10501 gtcggtgagg cgcgtgagca cgatgtgggc cgcgacgacg aggtctttgt aggtgaacct
    10561 gaagtcgagg cgctgggcgg aatcgccgaa ggccatgaag gcgaggaagc ggcccttgag
    10621 acggcgcagc tcctcgaccg gggcgatgtc ttcgaagaag tcgtgcccga cgacctcccg
    10681 cggctgaccg ttcgctgcgc cctccccgtg ggccggggtg aagcggatga ccatgcccgc
    10741 gccgtccagt tccaacagcc cgaatgggaa gcccgaagcc ttgacgttct ccaagatgaa
    10801 agcccccgcg tgaatcgttc tctctggtca accgccgcgc ggcggcgcaa aatcaaagag
    10861 ggcgagccgc cgaagacgac ccgccctcca accctcccct tgctcgtcct gcgacgcctt
    10921 gatcccttgc gaggctcaat acgaagccgc tttatgaaat gtgcgcgccg tccacgaggg
    10981 gacgcgccgg agtcaccctg cgagtcgagt atgaattgtc tgtcgcgcgg cggtgtcgca
    11041 cgccgcgcga gtcttcggcc tctcttcgtg ggggacgcgt gcgaatatat aaaacggtgc
    11101 gcgccgcgtc ggcacgcaat cgtaccgacg ttgaactcct cggacaccta atagcgcgca
    11161 cgctttaagg cagcgtagcg gaacctacaa ctcggggcgc gcgtcgctga cggcgcgggt
    11221 gccgctggcc gaggacgcgc gagcacttcg agggcgttga ccgcgagtcg tgcttcggcc
    11281 acatcaagaa ggcgtgaggc aatcgctttc gggttgaggc gtcccgtgtg ccgtctcgtc
    11341 aggcggctgg cctgtctgcg gatcgaagaa gaggcggcgg tgaatctccg cgacgcgctc
    11401 gggcgcgacc ttcggctggc ggtcgtaggt cagcaggccg ttgatctcct gctcgatgtc
    11461 cgtgagctgc gtgtagcaga agcccgcgat gaaggggagc gaggcgatgc cctccatcag
    11521 gtcgcggtac tttgcgagca ggtcttcgta cgtgcccgcc gagccgtaga agcggtagag
    11581 catgtcacgc cgctcgggcg gcacgtcggg cgggatcatg aggaagccgc cgacctccgt
    11641 gagcatcacg ggctggccgc ggtagcgcga gccgaggacg aagagcggtt tgttgccgat
    11701 ccacacgtgc gcgggcagcg tgctgccgcc cacggtctcg cggtagcgct cgcgcagcag
    11761 ctccgccgtc ggcgtgtagt cgtggatggc gacgatgtcc gtgatgtccg tgtgctccca
    11821 gccgtcgttg tcgatgacgg ggcggcgcgg gtcgagttgg cgggtcatcg ccaccatgcg
    11881 ctcgatgaag gcgtactgac cggggtgctg tttgcgcagg cccggaaagc ccatgctctc
    11941 gttgaccggc acccacgtga cgatgcacgg gtggttgtag tcgcgctgca cggcgcgctt
    12001 ccactcggcg gccagacgct cctcggcctg cgaagaccac tcgcgcgcgt tcggcatctc
    12061 ctcccacacg agcaggccca ggcggtcgca ccagtagagc cagcgcgggt cttcgatctt
    12121 ctgatgcttg cgcgcgccgt tgaagccgaa ccgcttgacc cacaacacgt ccgcgcgcaa
    12181 cgcctcgtcc gacggcgcgg ccagataact ctcgggccag tagccctgat ccaggatcat
    12241 cgccagatag accggctcgc cgttcaggcg cacgcgccca ccgcgcagct ccacgtcgcg
    12301 caggcccgcg tacgactcga cctcgtcgat cagctcgccg ccctcgaaca gccgcagccg
    12361 cagcccgtac aggtgcggcg agtcgggcga ccagagcctc gcttcgggga tgtgcaggtc
    12421 gagttgacag gtggcggcgg tcgtctcctc ttcggcgcgc gcgacgacgg cgcgggtgtc
    12481 gagttcgaac gcctctgctt cgagcctgcg ctcgacggag ggcgcgtgga ggtagaccat
    12541 gatgtcgagc gaactcgtcc gcgccgacgg cgtgagcttg atctcgccga tgcggacggg
    12601 cggaactggt tcgagccaca cggtctgcca gatgccggtc gtgcagtagt agtcgatgtc
    12661 gtgcggcagg cccgtcgcgg actgcttgcc gcgcggctgt cgcgggttct gccggtcttc
    12721 gacacgcacc gtcaggcggt tcggctcgtt cgacttcagg tacggcgcga tgtcgaagct
    12781 gaacggcacg tgcccgccct ggttgtgtcc gacctcctgc ccgttgaccc acagcgtcgt
    12841 gcggtaatcg accgcgccga agtggagcag cacgtcgcga ccgcgccact ccgccggcac
    12901 ctcgaaggtg cgcgcgtacc agacgacttc gtgaacggag ttgtcgtcga tgcccgaccg
    12961 gctcgactgg taaggaaagg gcacgacgat gcgcccttcg agagcgaggc cgtactgcca
    13021 gccgagcgag cgccccttgt tcgcgtcatc gaaggcgaac tcccattcgc cgttcaggtt
    13081 caaccactcc gcgcggacga actgcgggcg cgggtattcg ggccgaggtg tctccatcgt
    13141 tttcacaaag ccgccgtgct ttccgattgt cgaaggggag gtgcggcagg ataacaaacg
    13201 gacgaccgct cctcaaccgt cggccctccg ccgcgcgcga aacgacaacc tgtgtacacg
    13261 agcggacaga cgctcgcacg ccgccgcgtt ataagtctgc cgcgaaacgc aatcaacaac
    13321 gaaccagtta aggcgaacgg gccgcccgtc tcttgcgtga agaccgggcg gcctgtctcg
    13381 ccggacttag gcagcgagta tttccgccgc cgcgggcggc gcgtcgaacg ggggaggact
    13441 atgaacgcat tcgacttgct gatgatggat caccagaagg tgtcgggcat cttcgaccgt
    13501 ctcgaaatga acgacggcgg cgtggccggc agccgcgcgg aacttttccg gcagcttaaa
    13561 aacgaactcg acgtgcacgc gcacgtcgag gagacgattt tttacccggc cttgaaagag
    13621 gcagaggaga cgcgcgagct aatcgccgaa gcgtacaacg aacacgccgc cgtcaaagac
    13681 ctgctcgccg aaatggaggc gacggcgccg ggcgaaggcg ggtgggacga cctgctggcg
    13741 gagctgcgcg agaacgtcga gcatcacgtc gaggaggagg agggcgacct cttcgagcag
    13801 gcccgcctcg tcctcaccag cgagcaggcg gaggctctcg gcgcgcgcat ggagcgggag
    13861 aagcagcgtc gcatgaccgc caccgcctga acagcaggcg gcaggcagca ggcggcaggc
    13921 agaacgatga cgcaatttcg gttttcggct tacagtgaaa actcgcgctc ttcctgaaaa
    13981 ccgaaaactt gtttgctgcc tgctgctctg cctgccgctg gcagcgcctc gaagatcgac
    14041 ggcacctgca tcatgtcatg ctcgtaggcc gacgacgggc gccctgaatt taatcggccc
    14101 atcgg
//
LOCUS       DBNGOH010000884         2782 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1600055, whole genome shotgun sequence.
ACCESSION   DBNGOH010000884 DBNGOH010000000
VERSION     DBNGOH010000884.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2782)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2782)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2782
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1600055"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(268..2220)
                     /locus_tag="AC159P_17190"
     CDS             complement(268..2220)
                     /locus_tag="AC159P_17190"
                     /inference="COORDINATES: protein motif:HMM:NF012698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3697232.1"
                     /translation="MNVPRQHVYEFGDFRLDPGRRLLLRRDGRPVPLKPKIFETLLYL
                     VEHDGRVLEKGELMRAVWPDTAVEENNLSQNISALRRALGETPGQHRYIVTVPGRGFR
                     FVAAVSTRAETNALEGAAAVSADPAVKLPEAVSTVAGTDKPGGGPKSILRPFALACVL
                     VAGLGAAGLILWRARTAPAPAASPAPASAPVKTIAVLPFKPLVEGEQDQALELGMAET
                     LIAKLSNSREVVVRPISAVRRYGALEQDPQAAGRELNVESVLDGHIQRRGDHIRVSAR
                     LLSVGDGRQLWAGQFDEKMTDIFAVQNVISERVAAALALPLTGEERAHLTRRHTENVE
                     AYQLYLRGRYSLSKRLPGALRQAIEHFRQAITLDANYAPAYAGLSSAYAFQSSAEFGG
                     MPPREVMPKAKEAALKAVVLDDTLAEAHIAVGRAAVFERDWPTVEREYKRGLELDPDY
                     SEGRQLYALFLLRMGRLDEALVEARRAWEIEPSSANKIVIVGWTHYFRREYDEAIEQY
                     GQTLDMDPNNQPARFRLGLAYMQKGMPDRAVAELQRAANLSGGNARHVAFLGYAYALS
                     GEWGKAERLLNELQTRSRQTYVCPYDIAIIHLGLGDKERALEWLEKTDRERDDWLLQL
                     KVEPVMDSLRSEPRFRDLVRRVGLPQ"
     gene            2429..>2782
                     /locus_tag="AC159P_17195"
     CDS             2429..>2782
                     /locus_tag="AC159P_17195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697233.1"
                     /translation="MLTLVAAIAAAWPLTGFGQDSDPNGATQLNCDERPCDQIARGRA
                     AFHDRNLRQLGGNGRACSDCHLPSDGFQLSPEAARTRFDALQAARAHNPHADDPLFRP
                     VDADDFRINGESASDF"
BASE COUNT          485 a          960 c          840 g          497 t
ORIGIN      
        1 tgcgcacttc atcgagcgag tcgtgggaca ggcgctgttc ttggatctcg ccgtcgcgcg
       61 tatggcacca cttgatgagc tgttgccggg tgtgtaaatc cacgccacaa tagatggtca
      121 tggtgactct cctctcttgc attcaagccc gaacgcaaga gtctgccccg cggcagagga
      181 gagtcatctg tttcatcaca tcaggtcccc cgtcattggt gacaatgact ggaagacagg
      241 ccggtatgcg gcccgcactt cctccggtca ttgcgggagc ccgaccctcc gcacgagatc
      301 ccggaagcgc ggctccgagc gcaggctatc catgaccggc tcaactttga gttgcaggag
      361 ccagtcgtcg cgctcccggt cagtcttctc aagccactcc aaggcgcgtt ctttgtcccc
      421 cagccccaag tgaataatcg ctatgtcgta ggggcagacg taagtttgcc tcgacctcgt
      481 ctgcaactcg ttcaataacc gttccgcctt gccccactcc ccggacagtg cgtaagcgta
      541 gccgaggaag gccacgtgtc tggcgttccc gccgcttagg ttggctgccc tttgcagctc
      601 cgcgacggcc cggtccggca tgcccttctg catgtaggcc agcccgaggc gaaaccgagc
      661 cggctggtta ttcgggtcca tgtccagcgt ctgcccgtat tgttcgatgg cctcgtcata
      721 ttcgcgcctg aagtagtgcg tccacccgac gatcacgatc ttgttggccg aactgggctc
      781 tatctcccaa gcccgcctgg cctctactaa agcctcatcc agcctcccca tgcggagcag
      841 aaagagtgcg taaagctggc gaccctccga gtagtcgggg tcgagttcga ggccgcgctt
      901 atactcccgc tcgaccgtcg gccagtcccg ttcgaagacg gcggcgcgcc ccaccgcgat
      961 gtgcgcctcg gcgagcgtgt cgtctaacac cacggccttc aaggccgcct ccttcgcctt
     1021 cggcatcacc tctctgggcg gcatcccgcc gaactccgcg gacgactgaa acgcgtaagc
     1081 gctggacagg ccggcgtaag ccggcgcgta attcgcgtcc aaggttatcg cctgtctgaa
     1141 gtgttcgatt gcctgccgca aggcccccgg caacctcttg ctcagggagt agcggcctcg
     1201 caaataaagt tggtaggctt cgacgttctc cgtatggcgc cgcgtgaggt gggcgcgctc
     1261 ctcgcccgtc agcggcagcg cgagcgccgc cgccacccgc tcggagatca cgttctgcac
     1321 ggcgaagatg tccgtcatct tttcgtcgaa ctgccccgcc cacagttgcc tgccgtcgcc
     1381 gacactaagc aggcgcgccg acacgcggat gtggtcgccg cgcctttgga tgtggccgtc
     1441 caacaccgac tcgacattca gctcgcgccc cgccgcctgc gggtcttgtt ccagcgcgcc
     1501 gtacctgcgc acggcgctga tcgggcgcac gacgacctca cggctgttgc tgagtttggc
     1561 gatcaacgtc tcagccatcc ccagttccag cgcctgatcc tgttcgcctt ccaccaaggg
     1621 cttgaacggc agcacggcga tggttttaac gggcgcgctg gcgggcgcgg gagacgcggc
     1681 gggggccggc gcggtacgcg cccgccagag gatgaggccc gcggcgccca gcccggccac
     1741 gagcacacac gcgagcgcga agggtcgaag aatacttttc ggtccgcccc ccggtttgtc
     1801 cgtcccagcg actgtactca ccgcctccgg caacttcacc gccgggtcgg ccgagaccgc
     1861 ggccgcgccc tccagcgcat tggtctccgc gcgcgtgctg acggcggcga cgaagcggaa
     1921 gccgcgcccc gggacggtca cgatgtagcg gtgctggccg ggtgtttctc ccagcgcccg
     1981 ccgcagcgcg gagatgttct ggctgaggtt gttctcctcg acggcggtgt cgggccagac
     2041 cgccctcatc aactcgcctt tttcgagcac cctcccgtcg tgctcgacca ggtagagcag
     2101 ggtctcgaaa atctttggct tgaggggcac gggccgccca tcacgcctga gcagcaggcg
     2161 cctgccgggg tcgagtcgga agtcgccgaa ctcgtaaacg tgctgccggg gtacgttcaa
     2221 gactcacctt caagaagatt cagaaaaaat cagacgcatt ttaaagacat tcagaggggc
     2281 atgggaagag tatcggtgcg acccgacgaa gcgtcggtga gaatctaggg agaggaaacg
     2341 ccgcacgagg agccggaaag accctcggct cgaaaggaga caggccatgt atacacccaa
     2401 gatagccgca gtcaaggcct tcttatgtat gcttacacta gtcgccgcaa tcgccgccgc
     2461 ctggccgcta accggcttcg ggcaagactc ggacccgaac ggcgcgaccc agctcaactg
     2521 cgacgagcgg ccctgcgatc agatagcccg cgggcgcgcc gccttccacg accgcaacct
     2581 ccgacaactc ggcggcaacg ggcgcgcctg ctcggactgc cacctgccgt ccgacggctt
     2641 ccagctctcg cccgaagccg cgcgaacccg cttcgacgcg ctgcaagccg cacgcgcgca
     2701 caacccgcac gccgacgacc cgctcttccg ccccgtggac gccgacgact ttcggatcaa
     2761 cggggagagc gcgagtgatt tc
//
LOCUS       DBNGOH010000885         4532 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3250211, whole genome shotgun sequence.
ACCESSION   DBNGOH010000885 DBNGOH010000000
VERSION     DBNGOH010000885.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4532)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4532)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4532
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3250211"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..463)
                     /locus_tag="AC159P_17200"
     CDS             complement(<1..463)
                     /locus_tag="AC159P_17200"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF021988.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005534 - galactose binding [Evidence
                     IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase family protein"
                     /protein_id="HIR3697234.1"
                     /translation="MLVSDLHKLISVATYLSRAESGAFEPRGGRTSLLVSLFGGDAPE
                     LPARAAGLLSHLQETYGDDVGAAQKSLDRLGALCASFLEAYGDGHVRLIRAPARINIL
                     GEHVDYVSYLPTYSLPFGSREHDMLMMYRPSATGRVRGASRRGEFARFDFSL"
     gene            943..1806
                     /locus_tag="AC159P_17205"
     CDS             943..1806
                     /locus_tag="AC159P_17205"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF037026.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3697235.1"
                     /translation="MSPNAEELEIRLLEESDVAEAMRLKAAAGWNQTEADWLRLIRLC
                     PRGCFAATAGARLVGTTTTTVYGRELAWVGMVLVDVEFRRRGIATALVCAALEGLDRA
                     AVATVKLDATPAGAPVYEALGFETELRVERWVGAARAEASPARAAEGAPDPSHIFEFD
                     RRAFGADRSELLAALLEESCVTPALSTGADGRLRGYALARRGALADYVGPLVAEETET
                     AAALLDDLLNRLGAGRVYVDLNTTYANGARVLEARGFVKQRGLIRMRRGGGSGAGTSD
                     SVFAIAGPEVG"
     gene            1837..2643
                     /locus_tag="AC159P_17210"
     CDS             1837..2643
                     /locus_tag="AC159P_17210"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010263153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006043 - glucosamine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HIR3697236.1"
                     /translation="MESRVQRDEAEDGLVEQFQAGRLNVYVYESRAKMGAAAAAAVSA
                     EIRRLQGERGRAVGIFASAPSQNEFLAGLAEAPGVDWSKVKGFHLDEYLGMEDSAPQS
                     FRRFLLDRLVNKVDFGEFHGLRGEAEDAADECRRYAALLNEDPPDFAVLGIGENGHLA
                     FIDPPFCDFDDPEPVKVAELDEVCRVQQVHDGAFAALEDVPRDALSLTIPTIMSRPKL
                     FAIVPGPAKAQAIKGVIEGPIETACPASILRNHADAHLFIDRDSASHLSR"
     gene            2883..>4532
                     /locus_tag="AC159P_17215"
     CDS             2883..>4532
                     /locus_tag="AC159P_17215"
                     /inference="COORDINATES: protein motif:HMM:NF019552.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III domain-containing protein"
                     /protein_id="HIR3697237.1"
                     /translation="MKVNRQQTNSEGSVLRVALVALTLSSFLFLSWAAGAQVARAQAS
                     PGQTRQAPAVVRPRMLVGERDPLSGFQVLRARYDAGARPPDDLAGWALTYLLKGDESF
                     ARRAVEEMRRARTPERVGSRTYPEFVKWSLAFDWLYNYPGFDAALKDRVAGELVKAAA
                     QMLQDQSLRDPRLSMYHNYTVRYLTLTAFALTAVEGHPSVEAEAAPLRERTRRVWEHI
                     HDLTDFITPDGGYHESMDYQRITYAPMALLAELDRTALNADPALRYPVFRHYTATHLY
                     KALPDGTTSRLGDNEFPYMRSQDNVVLGYAVHRFKDPYAAWMLRESGWPDTKDWLIPI
                     TKFLWDDPVVAPRDPARTTEAELSRQHHFRGVGHLVMRDGFGPDSTWIEFVAGPYLAK
                     HQHLDQLHFAVYRKGYLATESGADYTDTESPHYLNYYRRTVAHNSVLVYDRRERFFWG
                     ENLWPAANDGGQRMDSSRYWNTARSREDFQNTRELWALARMESVGHRPGAYHYARGDA
                     TRAYSPAKVELFTRELVYTPADNVLFVFDRVRATDPAFRKTW"
BASE COUNT          772 a         1501 c         1520 g          739 t
ORIGIN      
        1 ctagcgagaa gtcgaagcgc gcgaactccc cgcgtcttga cgcgcccctg acgcgccccg
       61 tcgccgaggg cctgtacatc atgagcatgt cgtgttcacg gctgccgaac gggagtgagt
      121 acgttggcag gtaagacacg tagtctacgt gctcgccgag gatgttgatc cgcgccgggg
      181 cgcggatcag gcgaacgtgc ccatcgccgt aggcctccag aaagcttgcg catagcgcgc
      241 ccagccggtc gaggctcttc tgggcggctc ccacgtcgtc cccgtaagtc tcctggagat
      301 gtgagagcag ccccgccgcc cgcgccggca gttccggggc gtcacctcca aagagtgaca
      361 cgagcagcga cgtccgaccg cctcgcggtt cgaacgcccc cgactctgca cgggagaggt
      421 aggtggcgac ggatatgagc ttgtgtagat ctgagactag caccgttacg acttgagagg
      481 gctgaacgtt cctatcgact ccaaagcgtt ttcgagcaag aggacgggtt tgctgcgttg
      541 gggtttgtta gggactctga caaatacagc ttaccataaa ccggtggagc ggcgtccaag
      601 ccatctcgcg gcgccccgga gacgtgctta taccatcata cgctctctaa agccaaaggc
      661 tcacagcggc tcgggacgaa gcatacggtg aagacgtgtg ggggcgagag gcggacggca
      721 aagctacata aaagcaattc tctcaaggta tagacactcg tcccgacagc atgcgccttg
      781 cggcagcgct cgggttaatg gtataaaacc gttcccgaca atgtgtcgtg ctctgaagta
      841 tcgtggagcg ctccgcttcg atattcgtgt caacccccgg agtcgtttgg aaactacaga
      901 acgagagagt cttcggggtt tgtcaggaag gtaaacaaac cattgagtcc caacgccgag
      961 gagcttgaaa ttcgtctgct cgaagagagc gatgtcgccg aggcgatgag actgaaggcg
     1021 gctgccgggt ggaatcagac agaggcggac tggctcaggc tcatcaggct ctgcccgcgc
     1081 ggctgtttcg ccgccaccgc cggcgcgaga ctcgtcggga cgacgaccac caccgtgtac
     1141 gggcgggaac tcgcctgggt gggcatggtg ctcgtggacg tcgagttccg ccggaggggt
     1201 atcgcgacgg cgctcgtctg tgcggcgctc gaagggcttg acagggctgc ggtggcgacg
     1261 gtgaagctgg acgcgacgcc cgcgggtgcc cccgtctacg aggccctcgg cttcgagacc
     1321 gagcttcgcg tcgagcgctg ggtgggcgca gcccgggcag aagcctcgcc ggctcgggcg
     1381 gcggaaggcg cgcccgaccc gtcgcacatc ttcgagttcg accggcgcgc gttcggcgct
     1441 gaccgctcgg aattgctggc ggcgcttttg gaagagtcgt gcgtaacgcc ggcgttgagc
     1501 actggggcgg acgggcggct gcgcggctac gcgctggcga gacgcggggc gttggccgac
     1561 tatgtcgggc ctctggtggc ggaggagaca gaaacggctg ccgccctcct cgacgacttg
     1621 ctcaatcgtc tgggtgccgg gcgggtctac gtggacctga acacgactta cgccaatggc
     1681 gcgcgcgtgc tggaggcgcg cggcttcgtc aaacagcgcg gactgatccg catgcgtcgg
     1741 ggtgggggaa gcggtgcggg cacttccgac agcgtcttcg ccatcgccgg gccggaagtg
     1801 ggctgaggcg cggaatcttc cgcatgagga gtgaacatgg aatcacgagt gcagcgggac
     1861 gaggccgagg acggcctcgt cgagcagttt caggcggggc gcctgaacgt ctacgtctac
     1921 gagtcgcggg cgaagatggg cgccgccgcc gccgccgccg tgtcggcgga aattcgtcgc
     1981 ctgcaaggtg agcgaggccg cgccgtcggc atcttcgcct ccgccccttc gcagaacgag
     2041 ttcctggcgg gactggccga ggccccgggc gttgactggt ctaaagtcaa gggctttcac
     2101 ctcgacgagt atctggggat ggaggacagc gcgccgcagt ccttccgccg cttcctgctc
     2161 gaccgtttgg tgaacaaggt agacttcggc gagtttcacg ggctgcgcgg cgaggccgag
     2221 gacgccgcgg acgagtgccg caggtacgct gccctgctca atgaagaccc gccggacttc
     2281 gccgtgctcg gcatcggcga gaacggccac ctggccttca tcgatccgcc cttctgcgac
     2341 ttcgacgacc cggagccggt caaggtggcc gagctggacg aagtctgccg cgtgcagcag
     2401 gtgcatgatg gcgccttcgc ggcgctcgaa gacgtcccgc gtgacgcgct ctcgctgacc
     2461 atcccgacca tcatgtcgag gccgaagctg ttcgccatcg tgccggggcc ggcgaaggcg
     2521 caggcaatca agggtgtcat cgaggggccg atcgagacgg cgtgcccggc ttccattctc
     2581 aggaaccacg cggacgcgca cctcttcatt gaccgggact cggcctccca cctctcgcgc
     2641 tgaaggcgcg gacagtccgg ccaaagccgc aaagctttaa aggttgagag cggagatgat
     2701 gcttgcaaag agcgggcgcg ggttccgggg gctggtactc gtccgcgtcg cggcgttcct
     2761 ttcgacccgg cgaacgtcct caccgactac ctgcgcgcac gcacccgttc caagtcagac
     2821 gaggccgggc tgcgctcggt cgccagcctc taccagacca tcctcgaagt ggagcgggga
     2881 agatgaaggt taacaggcaa cagacaaact cggaggggag tgtgttgcgc gtcgcgctcg
     2941 tcgcgctcac gctctcctcc ttccttttcc tttcgtgggc agccggggcg caggtcgcgc
     3001 gtgcgcaggc ctcgccggga cagacgcggc aggcgccggc ggtcgtcagg ccgcgcatgc
     3061 tcgtcggcga gcgcgacccg ctgtcgggct tccaagtctt gcgcgcgcgt tacgacgcgg
     3121 gcgcgcgacc gccggacgat ctggccggct gggcgctcac ctacctgctc aagggcgacg
     3181 agtcgttcgc gcgccgcgcc gtcgaggaga tgcggcgcgc gcgcacgccc gagcgcgtcg
     3241 ggtcgcgcac ctatcccgag ttcgtcaaat ggtcgctcgc cttcgactgg ctctacaact
     3301 atccgggctt cgacgcggcg ctcaaggatc gcgtggccgg agagctggtc aaggccgccg
     3361 cgcagatgct tcaggaccag tcgctccgcg acccgcggct ctcgatgtac cacaactaca
     3421 ccgtcaggta tctcacgctc acggccttcg cgctgacggc ggtcgagggg cacccgtcgg
     3481 tcgaggcgga ggccgcgccg ctgcgcgagc gtacgcgccg cgtgtgggag cacatccatg
     3541 acctcacgga cttcatcacg cccgacggcg gctaccacga gtcgatggac taccagcgca
     3601 tcacctacgc cccgatggcg ctgctggccg aactcgaccg cacggccttg aacgcggacc
     3661 cggcgctgcg ctacccggtc ttccgccact acacggccac gcacctctac aaggcgctgc
     3721 ccgacggcac gacctcgcgt ctcggcgaca acgagttccc gtacatgcgc tcgcaggaca
     3781 acgtcgtgct cggctacgcc gtccaccgct tcaaagaccc ttacgccgcg tggatgctcc
     3841 gcgagtcggg ttggccggac acgaaagact ggctgatacc catcaccaaa tttctctggg
     3901 acgaccccgt ggttgcgccg cgcgacccgg cgcgcacgac cgaggcggag ctttcgcgcc
     3961 agcaccactt ccgcggcgtc ggccacctcg tcatgcgcga cggcttcggc cccgactcca
     4021 cctggatcga gttcgtcgcc gggccgtacc tggccaagca ccagcacctc gaccagctcc
     4081 acttcgccgt ctaccgcaag ggctacctcg ccaccgagag cggcgccgac tacaccgaca
     4141 cggaaagccc tcactacctc aactactacc gccgcaccgt cgcgcacaac tcggtgctcg
     4201 tctacgaccg gcgcgagcgt ttcttctggg gggagaacct ctggccggcg gccaacgacg
     4261 gcgggcagcg catggactct tcgcgctact ggaacacggc gcgcagccgc gaggacttcc
     4321 agaacacgcg cgagctgtgg gcgctcgcgc ggatggaatc ggtcggccac cggcccggcg
     4381 cctaccacta cgcgcgcggc gacgccacgc gcgcctacag ccccgcgaag gtcgagctgt
     4441 tcacgcgcga gctggtctac acgcctgcgg acaatgtcct cttcgtcttc gaccgcgtgc
     4501 gcgcgaccga cccggctttc aggaagacgt gg
//
LOCUS       DBNGOH010000886         4422 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3250437, whole genome shotgun sequence.
ACCESSION   DBNGOH010000886 DBNGOH010000000
VERSION     DBNGOH010000886.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4422)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4422)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4422
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3250437"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..942)
                     /locus_tag="AC159P_17220"
     CDS             complement(<1..942)
                     /locus_tag="AC159P_17220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006593794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HIR3697238.1"
                     /translation="METTLTLILGLGLTLLGLVWWRRAAAQGRALAPNARRSALAVAL
                     LLGAASFTSVSLAQRAPSDEARRPATEAAQAVTRGTPGGEANLVLPDLASVEFLGIDG
                     HTLLLIGIVVCALGLGFGMWIYTQLKNLPVHRSMREISELIYETCKTYLITQGRFIMI
                     LWLFIGAIAAIYFGFLAETVNPATGAIEHGFPALKVVVILLFSLVGIAGSYGVAWFGI
                     RVNTFANSRTAFASLGGKPYPIYAIPLKAGMSIGMMLISVELLIMLCILLFVPADYAG
                     PCFIGFAIGESLGAAALRIAGGIFTKIADIGSDLMKIV"
     gene            complement(1197..3158)
                     /locus_tag="AC159P_17225"
     CDS             complement(1197..3158)
                     /locus_tag="AC159P_17225"
                     /inference="COORDINATES: protein motif:HMM:NF012698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HIR3697239.1"
                     /translation="MIEEGRARSLYEFGPFVVDEGRRRLLRGGETVPLTKKEFETLLV
                     LVRGGGRVVEKGELMEAVWPDAHVEEGNLAVHVSKLRGKLGRREDGEPYIETETGRGY
                     RFTARVREVEDFDLVVRKRTRTHTVTEEEETDGPAAGAQAPSLPAANAPAGHRLTWRG
                     ALVVASAVAVAVAASAYFLQRRAAGRADAPADIRTLAVLPFALLDAGERDEYLGLGMA
                     DALITRLSNVRQIVVRPTSAVRGFAGPGRDAADAGRRLGVEAVLEGSIQKSGDRLRVT
                     AQLVRTADGASLWAGTFDEPLTNVFAVQDAISEQLAAALALELTSDERARLTRRYTEN
                     ADAYQLYVKGRYYWSKRDFDGVTRAESLFRQAIERDPNFAPAYVGLADMLAMGGPHHE
                     IDVLQAKALELAPDLAEAYASLGFTAMFHRWDWAGAERELHRAIELNPGYGTAHQWLA
                     TLLAITGRTEEAKAAMRRALEVDPQSPNFLADMAQMHYFAREYAEAESYSRRALEVAP
                     DFVFAHLYLTYIYLKTGRHAEALEEWCLTDRAAAPTKYDAAAAERQRREVLERHRREG
                     FIGFLRELTRDMPRDAHEAHDASLQYQLALYHAALGDRERALHWLEKAVAGRAFLAVF
                     FRADPVFDDLHAEPRYQALLRQANLPARP"
     gene            3401..>4422
                     /locus_tag="AC159P_17230"
     CDS             3401..>4422
                     /locus_tag="AC159P_17230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697240.1"
                     /translation="MSFTRRLPLTFALALALCAVAAAQTSEVSESARAEAPPVAVVAT
                     AERVRVSAPAAVVQLRLEVYDEAGRKVFDTEQRGGSVLDWHLQSGAGERVPDGTYVCV
                     VTIKSLNGRLSRRLGHVEVGEGQATARPFEAAQLSAAQAQAVGPVEESAGLSVLTPGG
                     AGAATVLAHNGDAGQLTRTRGDLSVRVGDFFAGTDREQMRLTEDGRLGIGTDKPEATL
                     DVAGEVRATEGFRFADGSKLSVDEKGALLRTSAAGAPLPGLGASVSAATANRLAKFAD
                     AAGTLIDSAITESGGNVGLGTAAPVGSLSIGGSTKPTPMTMNYQYFSPNSIVDQGLIG
                     WNYYWNG"
BASE COUNT          685 a         1490 c         1593 g          654 t
ORIGIN      
        1 gacgatcttc atcaggtcgg agccgatgtc ggcgatcttc gtgaagatgc cgcccgcgat
       61 gcggagcgcc gccgcgccca ggctctcgcc gatggcgaag ccgatgaagc aggggccggc
      121 gtagtcggcg gggacgaaga gcaggatgca gagcatgatc aaaagctcga ccgagatgag
      181 catcatgccg atggacatgc ccgccttcag ggggatggcg tagatcgggt agggcttgcc
      241 gccgagcgag gcgaaggcgg tgcgcgagtt ggcgaacgtg ttgacgcgga tgccgaacca
      301 ggcgacgccg taagaccctg cgatgccgac gagactgaag agcaggatga cgacgacctt
      361 gagcgcgggg aagccgtgct cgatggcgcc ggtcgcgggg ttgacggtct ccgcgaggaa
      421 gccgaagtag atggcggcga tggccccgat gaagagccac aggatcatga tgaagcgccc
      481 ctgcgtgatg aggtaggtct tgcaggtctc gtagatcagc tcggagattt cgcgcatcga
      541 gcggtggacg ggcaggttct tgagctgcgt gtaaatccac atcccgaagc cgaggccgag
      601 cgcgcagacg acgatgccga tgaggagcag cgtgtgcccg tcgatgccga ggaactcgac
      661 cgacgcgagg tcgggcagca cgaggttggc ctcgccgccg ggcgtgccgc gcgtgacggc
      721 ctgcgccgct tccgtcgcgg gccgccgcgc ctcgtccgag ggcgcgcgct gcgccaagct
      781 gacggacgtg aacgaggcgg cgccgagcag aagggcgacg gcgagggcgc tgcggcgcgc
      841 gttcggcgcg agcgcgcggc cctgcgcggc ggcgcggcgc caccagacga ggccgagcaa
      901 ggtcaggccg aggccgagaa tcagcgtgag cgtggtttcc acagttaaga caatcctttc
      961 agttcgtttg atgacaggcg agtcgaaagc ctttgaatta gcgggcgcga gccttccgtc
     1021 cgacaacggc gcccggacag accgacaccc cacgagagag ggttacttaa gtcctactgg
     1081 ccgttatttt ggactcgcca aaagcgggca ttatcgccga tttccaccct gactgtccac
     1141 acgggaggga acgtatcgag gcgcggaagg cggcggcgcg ggccgcgccg cgggggttaa
     1201 ggccgcgccg ggaggttcgc ctgccgcagc agcgcctggt agcgcggctc tgcgtgcagg
     1261 tcgtcgaaca ccgggtcggc gcggaagaag accgccagaa aggcgcggcc cgcgacggcc
     1321 ttctccagcc aatggagcgc ccgctcccgg tcgcccagcg ccgcgtggta gagcgcgagc
     1381 tggtattgga ggctggcgtc gtgtgcttcg tgtgcgtccc gcggcatgtc cctggtcagc
     1441 tcgcgcagaa aaccgatgaa accttcccgg cggtggcgct ccaggacttc gcgccgttga
     1501 cgctcggcgg ccgccgcgtc gtacttcgta ggcgcggcgg ccctgtccgt caggcaccac
     1561 tcttccagcg cctccgcgtg ccggcccgtc ttcaggtaga tgtacgtgag gtagaggtgc
     1621 gcgaagacga agtcgggcgc gacctccagc gcccggcggc tgtaactctc ggcctccgcg
     1681 tactcgcgcg cgaagtagtg catctgcgcc atgtcggcca ggaagttggg cgactgcggg
     1741 tctacctcca gcgcgcgccg catcgcggcc ttcgcctctt cggtgcgccc ggtgatggcc
     1801 agcagcgtcg cgagccattg gtgcgccgtc ccgtaaccgg ggttcagctc gatggcgcgg
     1861 tgcagctcgc gctcggcccc cgcccagtcc cagcggtgga acatcgctgt gaagccgagc
     1921 gaggcgtagg cttcggcgag gtcgggcgcg agttcgagcg ccttcgcctg gaggacgtct
     1981 atctcgtggt gcgggccgcc catcgccagc atgtcggcca gcccgacgta ggcaggcgcg
     2041 aagttcgggt cgcgctcgat ggcctggcgg aagagcgact cggcccgcgt cacgccgtcg
     2101 aagtcccgct tgctccagta gtagcggcct ttgacgtaga gctggtaggc gtccgcgttc
     2161 tcggtgtagc gccgggtcag ccgcgcgcgc tcgtcgctcg tgagttcgag cgcgagcgcg
     2221 gcggccaact gctccgagat cgcgtcctgc acggcgaaga cgttggtcag cggctcgtcg
     2281 aacgtgccgg cccacagcga cgcaccgtcc gccgtccgca ccagttgcgc cgtcacgcgc
     2341 aggcggtcgc ccgacttctg gatgctgccc tccagcacgg cctcgacgcc gagccgccgc
     2401 cccgcgtcgg cggcgtcgcg ccccggcccg gcgaacccgc ggacggcgct cgtcgggcgc
     2461 accacgatct gccggacgtt gctcaggcgc gtgatcagcg cgtcggccat ccccagcccc
     2521 agatactcgt ctctttcccc ggcgtcgagg agcgcgaacg ggagcacggc cagcgtcctg
     2581 atgtcggcgg gcgcgtcggc ccgccccgcg gcgcgccgct gcaaaaagta ggccgacgcg
     2641 gcgacggcga cggccacggc ggaggccacg accaacgccc cgcgccaggt gaggcggtgc
     2701 ccggcgggcg cgttcgccgc cggaagtgat ggcgcctgcg cgcccgccgc cggcccgtcc
     2761 gtctcctcct cctcagtgac agtgtgcgtc cgcgtgcgct tgcggacgac gaggtcgaag
     2821 tcctcgacct cgcggacgcg ggccgtgaag cggtagccgc gccctgtctc agtctcgatg
     2881 tagggttccc cgtcctcgcg ccgcccgagc ttgccgcgga gcttcgagac gtggacggcg
     2941 aggttgccct cctcgacgtg cgcgtcgggc cagaccgcct ccatcagctc gcccttctcg
     3001 accacgcgcc cgccgccgcg gacgagcacc agcagcgtct cgaactcctt cttcgtcagc
     3061 ggcaccgtct cgccgccgcg cagcagcctt cgccggccct cgtcaacgac gaacggcccg
     3121 aactcgtaga ggcttctcgc tcgcccttct tcgatcatgc gcgtccgccc cgtcttctta
     3181 agaaaatatt taagacgaat taatgcgaat ccaaaggact ttgcttccgg tgcgggcgca
     3241 ttatccgcgt ccgactcggg gcacgccggg agcaggtcgg tgtggcgccg attctacgcc
     3301 gggcgcgcgg gcggggcaag agtctcgcgc cgcacggtta gcccgcacga gtgctgcccg
     3361 tccgcaccgt gcgccgaaca cctctcgagg agtaaaggag atgtcattca cacgccgact
     3421 accgttgacc ttcgcactcg cgctcgcgct ctgcgccgtc gccgccgcgc agaccagcga
     3481 ggtgtcggaa agcgcgcgcg ccgaggcgcc acccgtggcc gtggtggcga ccgccgagcg
     3541 ggtcagagtc tccgcgcccg cggcggtggt gcagttgcgc ctcgaagttt atgacgaagc
     3601 gggccgcaag gtcttcgaca cggagcagcg cggagggagt gtgctggact ggcacctcca
     3661 gagcggcgcg ggcgagcgtg tgccggacgg cacttacgtc tgcgtcgtca cgatcaagag
     3721 cttgaacgga cgcctgagcc ggcggctggg tcacgtcgaa gtcggcgagg ggcaggccac
     3781 agcccgcccg ttcgaggcgg cacagctcag cgcggcgcag gcacaggccg tcgggccggt
     3841 cgaggaaagc gcggggctga gcgtgctgac gccgggcgga gcgggggcgg caacagtcct
     3901 ggcacacaat ggcgacgcgg ggcagctcac gcgcacgcgc ggcgacttgt cggtccgcgt
     3961 gggcgacttc ttcgccggca cagaccgcga gcagatgcgg ctgactgagg atgggcgcct
     4021 cggcatcggg acggacaagc cggaggcgac tctggacgtg gcgggcgagg tcagggccac
     4081 cgaaggcttc cgcttcgccg acgggagcaa gctgtccgtc gatgagaagg gggcgctctt
     4141 gcgcacctcg gcggcgggcg ccccgttgcc cggcctcggc gcgtcggtct ccgcggcgac
     4201 ggccaaccgc ctggcgaagt tcgcggatgc cgccggcacg ctcatcgact cggccatcac
     4261 ggagtctggc ggcaacgtcg gtctggggac ggcggccccc gtcggcagcc tgagcatcgg
     4321 cggctcgacc aagccgacgc cgatgacgat gaactaccag tacttctcgc ccaactccat
     4381 cgtcgatcag ggcctcatcg gctggaacta ctactggaac gg
//
LOCUS       DBNGOH010000887         3297 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_133867, whole genome shotgun sequence.
ACCESSION   DBNGOH010000887 DBNGOH010000000
VERSION     DBNGOH010000887.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3297)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3297)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3297
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_133867"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2021
                     /locus_tag="AC159P_17235"
     CDS             <1..2021
                     /locus_tag="AC159P_17235"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HIR3697241.1"
                     /translation="NRAKDGSLYWVDTTIVPFLDARGKPYQYVAIRYDITERKRAEEN
                     LHESRSRLSGVIESAMDAIITLDAGQRIVVFNRAAKQLFRCPATEALGRRLDRFIPVR
                     YRGAHGGHVSGFGETGVTTRVMAGARSVFGLRADGEEFPIEASISQTGSGDQKLYTVI
                     IRDITERKAAEERIREQTALLDAARDAIIVRDLEDRILYWNRGAERMYGWAEAEAVGA
                     GIVELLYQGNPAQYEQARRVVTEEGAWAGELRHATKGGKEITVESRWTLVRDERGEPK
                     SVLAINTDISERKRMETQFLRAQRMESIGTLAGGIAHDLNNILSPILMAVEMLRMKVT
                     DEDGQVCLALLQKNAERGGSMVKQVLSFARGVEGERVALQPQHLIKDVIKILGETLPK
                     AISIRLDLRERLEVVSADATQLHQVLMNLCINARDAMPAGGVLTIRAENKTVDENYAR
                     MQFDAQAGSYVAVTVEDTGTGMPPEVLNRIFEPFFTTKEMGKGTGLGLSTALTIVRSH
                     GGFVNVYSEVGKGTKFTFYLPVAESGEGAEAGKARPELPGGSGELILVVDDEEAIRRI
                     TKGTLETFGYKVLTAGDGTEAVALYAENSGEIKAVLTDMLMPFMDGLATIRALRRMNP
                     RVKVIAASGLTGNGQSAEAEAAGVKTFLAKPYTAEKLLKTLAELLSQP"
     gene            2268..3191
                     /locus_tag="AC159P_17240"
     CDS             2268..3191
                     /locus_tag="AC159P_17240"
                     /inference="COORDINATES: protein motif:HMM:NF012791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR3697242.1"
                     /translation="MITVERILCPVDLAHDAGGALGYAAALAQTFGARLLAYYCAKEL
                     PPERTLARHEKCEGLKSDIGRSFAAQAGASSIRSFAFESVVGEGRDAAEAIAREAAAR
                     RVDLIVMNSRRRPVAAALLGSTAERVCRMAPCPVLVTHPDERERVDGAGRRELRRILV
                     AYDFSNDSELALRHGLSLARAYETEMHLLHVMRRPERDGPEVLCRPDVLEGAYRAALQ
                     RLQKTVPEEAGLPRAVTTAVHWGKPYREILSYAREQDADLICMGASGAEFGMGTLFGS
                     NADRVLRQAPCPVLVTRPLKPAAFAPSDAQV"
BASE COUNT          609 a         1092 c         1112 g          484 t
ORIGIN      
        1 gcaaccgggc gaaggacggc tcgctctact gggtggacac gaccatcgtc ccgttcctcg
       61 acgcgcgggg caagccctac cagtacgtcg ccatccgcta cgacatcacc gaacgcaagc
      121 gcgccgaaga gaatctgcac gagagccgct cacgactctc cggcgtcatc gagtcggcga
      181 tggacgccat catcacgctc gacgcggggc agcgcatcgt cgtcttcaac cgagctgcca
      241 agcagttgtt ccgctgcccg gccacggagg cgttaggccg gcggctcgac cgcttcatcc
      301 ccgtgcgcta ccgcggcgca cacggcgggc acgtcagcgg gttcggcgag acgggcgtca
      361 cgacccgcgt gatggcaggg gcgcgctccg tcttcgggct gcgggccgac ggcgaggagt
      421 tccccatcga agcctccatc tcgcagaccg ggtcgggcga ccagaaactc tacaccgtca
      481 tcatccgcga catcacggag cgcaaggccg ccgaggagcg catccgcgag cagaccgcgc
      541 tgctcgacgc ggcgcgcgac gccatcatcg tccgcgacct cgaagaccgc atcctctact
      601 ggaaccgggg cgccgaacgc atgtacgggt gggccgaagc ggaggccgtc ggcgcgggca
      661 tcgtcgagct gctgtatcaa ggcaacccgg cgcagtacga gcaggcccgg cgcgtcgtga
      721 cggaggaagg cgcgtgggcg ggggaactgc gccacgcgac gaagggcggg aaggagatca
      781 ccgtcgagag ccgctggacg ctggtgcgcg acgagcgcgg cgaaccgaag tccgtgctcg
      841 ccatcaacac cgacattagc gagcgtaagc gcatggaaac gcagttcctc cgcgcgcagc
      901 gcatggagag catcggcaca ctcgcgggcg gcatcgcgca cgacctcaac aacatcctct
      961 cgcccatcct gatggccgtg gagatgctcc gcatgaaggt cacggacgag gatggacagg
     1021 tgtgtctcgc cctccttcag aagaacgccg agcgcggcgg aagcatggtc aagcaggtgc
     1081 tctcgttcgc gcgcggcgtc gagggagagc gcgtggcgct ccagccccag catctcatca
     1141 aagacgtgat aaaaatcctg ggcgagacgc tgccgaaggc gatcagcatc cgcctcgacc
     1201 tgcgcgaaag gttggaggtc gtctcggcgg acgccacgca gctccatcag gtgctgatga
     1261 acctgtgcat caacgcgcgc gacgcgatgc cggcgggcgg cgtcctcacg atcagggccg
     1321 agaacaagac tgtggacgag aactacgcgc ggatgcagtt cgacgcgcag gcgggcagct
     1381 acgtcgccgt caccgtcgaa gacaccggca cggggatgcc gcccgaggtc ttgaacagaa
     1441 tcttcgagcc gttcttcacg accaaggaga tgggcaaggg gacgggcctc ggactctcga
     1501 cggcgctcac catcgtgcgg agccacggcg ggttcgtcaa cgtctacagc gaggtgggca
     1561 aggggacgaa gttcaccttc tacctccccg tcgccgagtc gggcgagggc gccgaggcgg
     1621 gcaaggcgcg gcccgaactc ccgggcggga gcggcgagtt gatcctcgtc gttgacgacg
     1681 aagaggcgat ccgccggatc accaaaggca cgctcgaaac cttcggctac aaagtgctca
     1741 cggcgggcga cggcacggag gctgtcgcgc tgtacgccga gaacagcggt gagatcaagg
     1801 ccgtcctgac ggacatgctg atgccgttca tggacggcct ggcgaccatc cgggccttac
     1861 ggaggatgaa cccgcgggtg aaagtcatcg ccgccagcgg cctcaccggc aacggccagt
     1921 ccgccgaggc cgaagccgcc ggcgtcaaga cctttctggc gaagccctac accgcggaga
     1981 agctcttgaa gacgctcgcg gaactcctca gccagccttg aattccgaac ttcgacggcg
     2041 cgacacactc tgcgtccttc ccgaacttca acacgcgggg ccacaccgtc ggcgcgctca
     2101 cgtccggact gtcgtcggga gcgtgcgcat ttctacgccg cgcggtggtc atattccgca
     2161 tccgttatca cggaaccggc acgaaagcag tgctgacggc gcggcatgta tcttgcgaac
     2221 gaccgcagcg acagtgaggc tgagccgtcc gcggagccgt ttgcaagatg atcacggtcg
     2281 aacgaatttt atgtcccgtt gacctggcgc acgacgcggg gggcgcgctc ggttacgccg
     2341 cggcactggc gcagacgttc ggcgccaggc tgcttgcgta ctactgtgca aaggagttgc
     2401 cgcccgagcg cacgctcgcg cggcacgaaa agtgtgaggg gttgaagtcg gacatcgggc
     2461 gatctttcgc ggcgcaagcc ggtgcctcca gcattcgaag tttcgcgttc gagagcgtcg
     2521 tcggcgaggg acgggacgcg gcggaagcga tagcgcgcga ggccgccgcg cggcgcgtcg
     2581 atctgatcgt catgaattca cgccgccgcc ccgtggccgc ggcgctgctc ggttccaccg
     2641 cggagagggt ctgccgcatg gccccgtgcc cggtcctcgt cacgcaccct gatgagcgcg
     2701 agcgggtgga cggcgcgggc cggagagaac tccgtcgtat cctcgtcgcc tacgacttct
     2761 cgaacgactc ggagctggcg ctccgccacg gcctttcgct cgcgcgagca tacgagacgg
     2821 agatgcacct gctgcacgtc atgcgcaggc cggagcggga cggccccgag gtcttatgca
     2881 ggccggatgt gcttgagggc gcttaccgcg ccgcgctgca acgtcttcag aagacggtgc
     2941 cggaagaggc gggactgccg cgcgcggtga cgaccgcagt ccactggggg aagccctacc
     3001 gggagattct ctcctacgcg cgcgagcagg acgccgacct catctgcatg ggcgcgagcg
     3061 gggcggaatt cggcatgggc acgctcttcg gctcgaacgc cgacagagtc ctgcgacagg
     3121 cgccctgccc cgtgctcgtc acacgcccgc tcaagccggc ggccttcgcc ccgtccgacg
     3181 ctcaagtctg actttcttca tcactcgaat tagaaaactg gcgcggccaa tctcccggtc
     3241 ggaaaattcc gcccgcgacg cgggaattta cgctttcctc agcctaagcg gcaaaga
//
LOCUS       DBNGOH010000888         1575 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4899267, whole genome shotgun sequence.
ACCESSION   DBNGOH010000888 DBNGOH010000000
VERSION     DBNGOH010000888.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1575)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1575)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1575
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4899267"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1575
                     /locus_tag="AC159P_17245"
     CDS             <1..>1575
                     /locus_tag="AC159P_17245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697243.1"
                     /translation="PSEEVAFIAGPQLKSGSPLNPTKWFDQTAVEKGLQHGEAFPAEP
                     PYKPLTGTVMATAGQKRITGSGTRFLSELRPGNLDHIVISGPRGGRFQYVVSTIESDT
                     VLNVSVAVAPGAAGSGLAAGRNTGEEQAAYDNLNYYDQALVQYVNCYRTGEARFCGYA
                     RKIADSWWRSGAINEGRTPFEESFAPRSSSLAGLMLRALDDRPEMFPWITEYVRQQLG
                     TWIERPLKLWYRPLSDAQLRGEEPRPVGEQMVRRKVGFYFGVRDGGYTLLHAANLARV
                     HPDPAVRAELRAKVLDAAVNYYARLQRLQSEDGGFYFELDGMEGLTTQPFQEGILAEG
                     LIQVHRLTGDETVKQSILKLAEHLWNRAYNKTRWRAFYYFVGGKFTDGTSCANGCGAA
                     ANPWPPSDKGQVAEARQLNPTAVHTFGYAYLISGDPKFRTWGDEVFDSSYAGRDGFRS
                     LAGFRAKEYDEAYRSAGKYLAWRLAHGNTQPGAATTGASQASSVAPTDPAPAANSETS
                     AAPVAPAPGEPSALVSA"
BASE COUNT          277 a          531 c          534 g          233 t
ORIGIN      
        1 cccgtcggaa gaggtggcct tcatcgccgg gccgcagctt aaatccggga gcccgctcaa
       61 cccgaccaag tggttcgacc agaccgccgt cgagaagggc ttgcagcacg gcgaggcttt
      121 ccccgcagaa cccccctaca agccgcttac gggcaccgtg atggctacgg ccggccagaa
      181 gcggatcacc ggctccggca cccggttcct gagcgaactc aggccgggta atctggacca
      241 catcgtcatt tcggggccgc gaggggggcg gtttcagtat gtcgtctcga ccatcgagag
      301 cgacacggtc ttgaacgtct ctgtggcggt ggctccgggc gcagccggct cgggactcgc
      361 ggcgggccgc aacaccggcg aggagcaggc ggcctacgac aacctcaact actacgatca
      421 ggccctggtg caatacgtca actgctaccg gacgggggag gcgcgcttct gcggctacgc
      481 ccggaagata gccgacagtt ggtggcgctc gggcgcgatc aacgaggggc gcaccccgtt
      541 tgaagagagc ttcgcgcccc ggagttcttc cctcgccggg ctgatgctgc gggccttgga
      601 cgaccgcccc gagatgttcc cctggataac cgagtacgtc cgtcagcaac tcggcacctg
      661 gatcgagcgc ccgctcaaac tgtggtacag gccgctctcg gacgcgcaac tgcgggggga
      721 agagcctcgc cccgtaggcg agcagatggt gaggcgaaag gtcggcttct acttcggcgt
      781 gcgcgacggg ggctacacgc tgctgcacgc cgcgaactta gccagagtcc accccgaccc
      841 cgccgtccgc gccgagctgc gggcgaaggt cttggacgcg gcggtcaatt actacgcccg
      901 tctacagagg ctccagtccg aggacggcgg cttctacttc gagctggacg ggatggaagg
      961 gctgacgacc cagcccttcc aggagggcat cctcgccgag gggctgattc aggttcaccg
     1021 gctcaccggc gacgagacgg tcaaacagtc gatcctcaag ctcgccgagc acctgtggaa
     1081 tcgggcctac aacaagacgc ggtggagggc cttctactac ttcgtcggcg ggaagttcac
     1141 ggacggcacg agctgcgcga acggctgcgg ggccgcggcc aacccgtggc ccccttcgga
     1201 caaggggcag gtggccgagg cccggcagct taaccccacg gcggtgcaca cgttcggcta
     1261 cgcctatctg atttcgggcg acccgaaatt caggacgtgg ggcgacgagg tattcgactc
     1321 cagttacgcg ggccgggacg gattcagaag cctcgccggc ttcagggcta aagagtacga
     1381 cgaggcgtac cgctcggcgg gcaagtacct ggcgtggcgg ctggcacacg ggaacactca
     1441 gcccggcgcg gcgacgacgg gcgcgtcgca ggcttcgtcc gttgcgccga ccgaccccgc
     1501 gccggcagca aactccgaga cctccgccgc gcccgtcgcc cctgcgccgg gcgaaccgag
     1561 cgccctcgtc tcggc
//
LOCUS       DBNGOH010000889         2206 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4716677, whole genome shotgun sequence.
ACCESSION   DBNGOH010000889 DBNGOH010000000
VERSION     DBNGOH010000889.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2206)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2206)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2206
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4716677"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(17..1129)
                     /locus_tag="AC159P_17250"
     CDS             complement(17..1129)
                     /locus_tag="AC159P_17250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015346855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697244.1"
                     /translation="MATERLDVIAEARPRAAAREWWLFSPRVDAGVFLGSALASLALL
                     WVGARAGVLDADAPDWAWVPAVLLVDVAHVYATAFRVYFDTEELKRRAWLYALAPAVA
                     LGVGVALYSESDKLFWRALAYLAVFHFVRQQYGWVALYRARLGERGRAGWWVDAAAVY
                     MATLYPLVYWHAALPRNFWWFLPGDFAGLPSAVERIAWPLYCIALGAYVVRSAYRWVV
                     AGAGNPGKDVVVLTTAVCWYAGIVAFNSDYAFTVTNVIIHGVPYMALVWWYARRRAPA
                     AGRPYRWLTRWPIVFVATLWALAYAEELLWDRALWHERAWLFGAAWDVSAWKVFLVPL
                     LALPQMTHYVLDGFIWRRKSNPTLALFTRAPEARGD"
     gene            complement(1253..1819)
                     /locus_tag="AC159P_17255"
     CDS             complement(1253..1819)
                     /locus_tag="AC159P_17255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006077939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HIR3697245.1"
                     /translation="MAGTGEPAGRAGRRAMIGLPRGTVLVVAYRPEWKSLFEEEAARL
                     RSLMGAAALGVEHVGSTSVEGMDAKPLIDLLVGVRDLGEARGWIPELEAHGYEFRPDA
                     DIPDRLFFAKGPRAWRTHHLSLAEPSSDFYTKHLRFRDYLRAHPAAREAYRALKHGLA
                     RDFPTDRASYTEGKRAFVEHILELAQES"
     gene            complement(1841..2059)
                     /locus_tag="AC159P_17260"
     CDS             complement(1841..2059)
                     /locus_tag="AC159P_17260"
                     /inference="COORDINATES: protein motif:HMM:NF014124.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RyR domain-containing protein"
                     /protein_id="HIR3697246.1"
                     /translation="MNLPDIEIVAAKVHEAWMDSKKAQGVTTRKSETGEELMVPYEQL
                     SEQAKDLDRSSVRAVYDAINATLDDEGA"
BASE COUNT          359 a          799 c          725 g          323 t
ORIGIN      
        1 agttcaacct cccggttcaa tcgcccctcg cctcgggagc tcgtgtgaag agcgcaaggg
       61 tcgggttact cttccgccgc cagatgaaac cgtccagcac gtagtgcgtc atctgcggca
      121 gcgcgagcag cgggacgagg aagaccttcc acgcagacac gtcccacgcc gcgccgaaca
      181 gccacgcgcg ctcgtgccac agcgcccgat cccacagcag ctcttccgcg taggccagcg
      241 cccacagcgt cgcgacgaag actatcggcc agcgcgtaag ccaacggtaa ggcctacccg
      301 cggcgggcgc gcgccgccgc gcgtaccacc agacgagcgc catgtacggc acgccgtgga
      361 tgatgacgtt cgtgaccgtg aaggcgtagt cggagttgaa ggcgacgatc cctgcgtacc
      421 agcagacggc ggtcgtcaag accaccacgt ccttgccggg gttgcccgcg cccgcgacga
      481 cccaccggta ggcggagcgc acgacgtaag cgccgagcgc gatgcagtag agcggccacg
      541 cgatcctttc gaccgccgac ggcagcccgg cgaagtcgcc cggcaggaac caccagaagt
      601 tgcgcggcag cgcggcgtgc cagtagacga gcggatagag ggtcgccatg tagacggcgg
      661 cggcgtccac ccaccacccc gcgcgcccgc gctccccgag gcgcgcgcgg tagagcgcaa
      721 cccagccgta ctgctgccgc acgaagtgga agacggcgag gtaggcgagc gcgcgccaga
      781 agagcttgtc gctctccgag tagagcgcga cgccgacgcc aagcgcgacc gccggcgcga
      841 gcgcgtagag ccacgcgcgc cgcttcaact cttccgtgtc gaagtagacg cggaaggccg
      901 tcgcgtagac gtgcgccacg tccaccagca gcaccgccgg cacccacgcc cagtccggcg
      961 cgtccgcgtc gagcacgccc gcccgcgcgc ccacccacag cagcgccagc gaggccagcg
     1021 cgctccccag aaacacgccc gcgtccacgc gcggcgagaa gagccaccac tctctcgccg
     1081 cggcgcgggg ccgagcctcc gcgatcacgt ccagtcgctc ggtcgccata gggtcggact
     1141 gccgtgcact ttacctctcg tcgaacggcg ggcgcgcccc gccgctcgaa cccgagaggt
     1201 aaagtgcgcg ggattatact tcaagcgacg ggcttgtcgg ccatcgccct tcttagctct
     1261 cttgggccag ttcgagaatg tgttcgacga aggcgcgctt gccctctgtg taagacgcgc
     1321 ggtcggtcgg aaaatcgcgc gcgagtccgt gtttgagcgc gcggtacgcc tcgcgcgccg
     1381 cggggtgcgc ccgcaggtaa tcgcggaagc ggaggtgctt cgtgtagaag tccgaggacg
     1441 gctccgcgag cgagaggtgg tgcgtgcgcc acgcgcgcgg ccccttggcg aagaagagac
     1501 ggtccgggat gtccgcgtcg ggccggaact cgtagccgtg cgcttcgagt tcgggaatcc
     1561 agccgcgggc ctctccgagg tcgcggacgc cgaccagcag atcgatgagc ggcttggcgt
     1621 ccatgccttc gacggaggtg ctccccacgt gctcgacgcc gagcgccgcc gcgcccatca
     1681 gcgagcgcag ccgcgccgcc tcttcttcga agagcgactt ccactcgggc cggtacgcca
     1741 cgacgagcac cgtgcctctc ggtagtccga tcatcgcccg tcgccccgcg cggcccgcag
     1801 gctcgcccgt cccggccatc acctcgtcgc cgcccgcctc tcaggcgccc tcgtcgtcga
     1861 gggttgcgtt gatcgcgtcg tacacggcgc gaacgctcga ccggtcgagg tctttggctt
     1921 gctcggaaag ctgctcgtaa ggcaccatca gctcctcacc cgtctcgctc tttcgtgtgg
     1981 tcacgccctg tgcttttttg gaatccatcc aggcctcgtg gactttggcc gcgacgattt
     2041 cgatatccgg tagattcatg tctctcctct tgagcgcgcc cgtcgggcgc cggatgatgc
     2101 cgccgggcaa tggtctccgg cgatgcgcgc cgcccggcac gcgggcgaac gcgcgcggcc
     2161 cgactttgca acgcggggcc ggtttcaatt acccttcaga accgat
//
LOCUS       DBNGOH010000890         2923 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1235715, whole genome shotgun sequence.
ACCESSION   DBNGOH010000890 DBNGOH010000000
VERSION     DBNGOH010000890.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2923)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2923)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2923
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1235715"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="AC159P_17265"
     CDS             <1..240
                     /locus_tag="AC159P_17265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014396371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697247.1"
                     /translation="DLVSHFEGEERTARRFLVVPYPVPRRCAAAYFPEANVLVPVRSV
                     ADKSNTPASKSVVVSLSPSTEPLKFDYDEVDGKSI"
     gene            358..924
                     /gene="efp"
                     /locus_tag="AC159P_17270"
     CDS             358..924
                     /gene="efp"
                     /locus_tag="AC159P_17270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HIR3697248.1"
                     /translation="MGANELKRKMMIKVDGEPYAVVEVFYASPTARGATTMVRTRLRH
                     LLTGAVQEKSFRSGDKFEEPDVVLAPASFLYADADGFHFMDEKTYEQFMLPAAEVGDD
                     RGYLKDGTSVQVMRYEGDPVALQLPQFVELTVAATEPGIKGDTAGAGRDLKPATLETG
                     LQVRVPLFIKEGERVHVNTQTGEVAGRA"
     gene            complement(1020..1523)
                     /locus_tag="AC159P_17275"
     CDS             complement(1020..1523)
                     /locus_tag="AC159P_17275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697249.1"
                     /translation="MKTRFFALGLALALVMTQAAPALAAGKKTAAAATPPPADWSAVQ
                     AIPRGEKIVLRMKDGKRHEGRFESASDLLVNIVRDGRTMSVERERIRRVSRKGGMSRT
                     NGALWGLAIGGGSGALTGGILYDAANGDFVESFVPACALLGAGIGAGIGALFGKGKKD
                     ITIYEAP"
     gene            1877..>2923
                     /locus_tag="AC159P_17280"
     CDS             1877..>2923
                     /locus_tag="AC159P_17280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697250.1"
                     /translation="MRCKTFFRACALAACVWLAGAAASGDVGLAGAGQETPKQEQGKD
                     NKQGPKLPPDEAKAANKIITATDAAGKLAASAEFLKKYPKSEVRRQVAEHVAAHVSAV
                     QDAGQRITLGEQYTAVFNGPGEADLVAGPVLDAYITTERNADAFRAGAAYLQAHPDEV
                     GVMRSLAVAAGNAAIKGNTEYVAQGRQYAAKAAELIEADKKPAAWEAAQWAEYKTKWL
                     PAIYREAGVLAMRAGDSAASKANMEKAAALKSTDPVVYIILSDAANQEYSAAARAYQA
                     MPAGAEKTARLKEVEGLLDRVIEVSAQAVAMMEGNAQYDAARQQIRQDLEGYYKYRRG
                     STQGLQELIDKYKKP"
BASE COUNT          542 a          964 c          965 g          452 t
ORIGIN      
        1 gacctcgtca gccacttcga gggcgaggag cgcaccgcgc gccgcttcct cgtcgtgccc
       61 taccccgtcc cgcgccgctg cgccgccgcc tacttccccg aagccaacgt cctcgtcccc
      121 gtccgcagcg tcgccgacaa gagcaacacg cccgcctcca agtccgtcgt cgtctccttg
      181 agtccctcca ccgagcctct caagttcgac tacgacgagg ttgacgggaa aagtatttga
      241 agagctttta gcttttggct gttagctgtt agctttggat cagcggcgat gtcggcaatc
      301 ggctaacagc taacagctaa cagctatcat tgatggaggg ccaaacgagt ggctttgatg
      361 ggtgcgaacg agttgaagcg gaagatgatg atcaaggtgg acggcgagcc gtatgcggtg
      421 gtggaggtgt tctacgcctc gccgacggcg cgcggggcga cgacgatggt gcggacgcgg
      481 ctgcgccacc tgctgacggg cgcggtgcag gagaagagct ttcgctcggg cgacaagttc
      541 gaggagccgg acgtcgtgct cgcgcccgcg tcgttcctct acgcggacgc cgacggcttc
      601 cacttcatgg acgagaagac ctacgagcag ttcatgctgc ccgccgcgga ggtcggcgac
      661 gaccgcggct atttgaagga cgggacgagc gtgcaggtga tgaggtacga gggcgacccc
      721 gtcgcgctcc agctcccgca gttcgtcgag ctgacggtcg ccgcgaccga gccgggcatc
      781 aagggcgaca cggcgggcgc cgggcgcgac ctcaaaccgg ccacgctcga aacgggcttg
      841 caggtgcgcg tccccctctt catcaaggag ggcgagcgcg tccacgtcaa cacgcagacg
      901 ggcgaggtcg cgggccgcgc ctgaacgatc tcggatttta gaatttggat tggtgttcga
      961 cgattgccga cagcccggct cccatcaatc gtcagtcgct aatccgcaat ccgcaatcat
     1021 cagggcgctt cgtagatcgt tatatccttc ttgcccttgc cgaagagcgc gcctatcccg
     1081 gcgccgatgc ccgcgccgag cagcgcgcag gcggggacga aggactcgac gaaatcgccg
     1141 ttggccgcgt cgtagaggat cccgccggtg agtgcgccgc tgccgccgcc gatggctaaa
     1201 ccccacagcg cgccgttcgt gcgcgacatg ccgcccttgc gcgagacgcg ccggatgcgc
     1261 tcgcgctcga ccgacatcgt cctgccgtcg cggacgatgt tgacgagcag gtcgctcgcg
     1321 ctctcgaacc ttccttcgtg gcgcttgccg tccttcatcc ggagcacgat cttctcgccc
     1381 ctcgggatgg cctgcacggc tgaccagtcg gcgggcggcg gggttgccgc cgccgcggtc
     1441 ttcttacccg cggcgagcgc gggcgccgcc tgcgtcatga cgagcgccag cgcgaggccg
     1501 agcgcaaaga atcttgtctt catcttcgat ctcctaaaaa atcaagcgcg tagagcttcc
     1561 gcgccgaaac aacggcaagg gccgtgcctc tcacgaaacc ctttcgaata cgaaccctcg
     1621 ccgccaccgc gtgcggccca gccgcaacaa tgctccgaca gttgtacttg aagggcggaa
     1681 ggccgcacgg ggaaaagggg aaaaggggaa gaggcgaaag ggggaagaca aaccgttctt
     1741 cgccccttcc tcttcttcct tttcccctct gccccttttc ccctttcgcc ccttcttccc
     1801 tgcttttcga tggtgtaagc taggggcgcc gttttcgaca gccggacaaa atcgagagaa
     1861 caggagtggt gggatgatgc gctgtaagac attcttccgc gcgtgcgcgc tcgcggcgtg
     1921 cgtgtggctg gcgggcgccg cggcgtccgg ggacgttggt ctcgcgggcg cggggcagga
     1981 gacgccgaag caggagcagg ggaaggataa caagcagggg ccgaagctgc cgccggacga
     2041 ggcgaaggcc gccaacaaga tcatcacggc gaccgacgcg gcgggcaaac tcgccgcctc
     2101 cgccgagttt ctgaagaaat atccgaagag cgaagttcgc cgtcaggtcg cggagcacgt
     2161 ggccgcgcac gtcagcgccg tgcaggacgc ggggcaacgc atcacgctcg gcgagcagta
     2221 caccgccgtc ttcaacgggc cgggcgaggc cgacctcgtc gctggcccgg tgcttgacgc
     2281 ctacatcacc accgagcgca acgccgacgc cttccgcgcg ggcgccgcct accttcaggc
     2341 gcacccggac gaagtaggcg tgatgcggag cctcgccgtc gccgccggca acgcagccat
     2401 caagggcaac accgaatacg tcgcgcaggg gcggcagtac gccgccaagg ccgccgagct
     2461 gatcgaggcc gacaagaagc ccgccgcgtg ggaggccgcg cagtgggccg agtacaagac
     2521 caagtggctg cccgccatct accgcgaggc gggcgtgctc gccatgcgcg cgggcgacag
     2581 cgccgccagc aaggcgaaca tggagaaggc cgccgcgctc aagtccacag acccggtggt
     2641 ctatatcatc ctcagcgacg cggccaatca ggagtacagc gcagcggccc gggcgtatca
     2701 ggcgatgccg gccggggccg agaagacggc gcggctcaaa gaggtcgagg gtctgctcga
     2761 ccgcgtcatc gaagtctccg cacaggccgt ggccatgatg gaaggcaacg cgcagtacga
     2821 cgcggcccgc cagcagatca ggcaagacct cgaaggctac tacaagtacc gccgcggctc
     2881 gacgcagggg cttcaggagt tgatcgacaa gtacaagaag ccc
//
LOCUS       DBNGOH010000891         4021 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4166104, whole genome shotgun sequence.
ACCESSION   DBNGOH010000891 DBNGOH010000000
VERSION     DBNGOH010000891.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4021)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4021)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4021
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4166104"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..807
                     /locus_tag="AC159P_17285"
     CDS             <1..807
                     /locus_tag="AC159P_17285"
                     /inference="COORDINATES: protein motif:HMM:NF051698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HIR3697251.1"
                     /translation="ALAALQPLRPDPSRTQVEQLVAGGDSFYHGLNLSARRRFARRTN
                     GFGLSLRASYTLSRLIDDGVVNTSSAVRPGDFRSERSRSLLDRRHRLALSAVLDAPPA
                     LGALRLSSIFRLASGAPFNLSLGGADRNLDDVSNDRPDFTGDLSLIRHRRPGTPADPR
                     LVAAFSLPLVGQTGNLPRNAGRGPSLFTFDVSLTREFRFGDRARLRPFVEIDNVPNKR
                     VFTFGAEFINFDALRADATEEQRRAFADTFLVPTRTLRPRTVRLGVRFDF"
     gene            1449..1721
                     /locus_tag="AC159P_17290"
     CDS             1449..1721
                     /locus_tag="AC159P_17290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697252.1"
                     /translation="MPEDTPDSGAKVTLDADTASKMESVLRDGLSGSQAYIADDEPDD
                     QGKKKAASVELDAPTVAQFADHLKERLSASGAYIAEDDAGEGAEKK"
     gene            1789..3015
                     /locus_tag="AC159P_17295"
     CDS             1789..3015
                     /locus_tag="AC159P_17295"
                     /inference="COORDINATES: protein motif:HMM:TIGR00702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HIR3697253.1"
                     /translation="MGQTFRLSSSLRAEPLEVTLARARAVAGWLGITRVTEITRLDRV
                     GVPVFASIRPNALPGSLCVNAGKGVSVMEARVGAYMEAVEFAFAEYGRSGLEVHAAPA
                     RQVYEGPDRPDAILDFCPVMRAEVALDATVACVEAADIVSGEKFPVPAELVFLPFPGA
                     LRTPRYFSSNSNGLCSGNSVLEATVHGLAEVIERDCISFMLVDDQSRLVAAPTLPPPL
                     RDLAAQVSAAGLELYLRHQSNVFGVPFFRAVVAEQGCTDPVYISEGYGCHLSRDIAAT
                     RAVCESLQSRLSFIHGGRDDLIKRYKLFGSRAENERAAYAERLLGEVSGSAGAIDFEE
                     TADCSDVATDLASALAVLTAKLAQNGIRRVLRVIYTPADYPVHVARILAPGLECFNHT
                     TARVGLRLRDHVRSRL"
     gene            2996..3736
                     /locus_tag="AC159P_17300"
     CDS             2996..3736
                     /locus_tag="AC159P_17300"
                     /inference="COORDINATES: protein motif:HMM:NF019429.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfuA-like protein"
                     /protein_id="HIR3697254.1"
                     /translation="MSDRVSNRFMFAGPSFCGEERLTAEAGGMMRVLPPVRRGDVESL
                     VSAHPPGVLVLVDGNFHQCLSVGHAEIRAAIEAGWSVWGLSSMGAIRAYEMRHLGMKG
                     YGRVYRCFYEHEDFRDDEVALVHEPVPPYHAVSEPLVHIRFFLRELVSRRLLTEPSER
                     AILETFMSLWFGDRTLPLLRSILLETTPRHEEEVADLLANFRRFRVKCRDLADFLRER
                     PWGASRRAGYEERPVNRSSSTTRESPNR"
     gene            complement(3676..>4021)
                     /locus_tag="AC159P_17305"
     CDS             complement(3676..>4021)
                     /locus_tag="AC159P_17305"
                     /inference="COORDINATES: protein motif:HMM:NF047558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TPR end-of-group domain-containing protein"
                     /protein_id="HIR3697255.1"
                     /translation="FERADVLAGKDAGQAARRAASLKEAHASRGERGFWRKSCELLKQ
                     DRERGDGVSPYALATSYAHLGDDDSAIEWLRRAYEERERLLLQLGTEPCFDHLLGDSR
                     VVELLRLTGLSS"
BASE COUNT          666 a         1381 c         1248 g          726 t
ORIGIN      
        1 gccctcgccg ccctccaacc ccttcggcca gacccctcgc gcacgcaggt cgaacaactc
       61 gtcgcgggcg gcgacagctt ctaccacggc ctcaacctct ccgcccgccg ccgcttcgcg
      121 cgacgcacga acggtttcgg cctctcgctc cgcgcctcct acacgctctc gcgcctcatc
      181 gacgacggcg tcgtcaacac ttcgagcgcc gtgcgccccg gcgacttccg ctccgagcgc
      241 tcacgcagcc tcctcgaccg ccgccaccgc ctcgccctct ccgccgtcct cgacgcgccg
      301 cctgcattag gggcactgcg cctctcatcg atcttccgcc tcgcctccgg cgcgcccttc
      361 aacctcagcc tcggcggcgc cgaccgcaac ctcgacgacg tgagcaacga ccgacccgac
      421 ttcacaggcg acctctctct catccgccac cgccgccccg gcacgcccgc cgacccgcgc
      481 ctcgtcgcag ccttctcgct cccgctcgtc ggccagaccg gcaacctccc ccgcaacgcc
      541 gggcgcggcc cctcgctctt caccttcgac gtgagcctca cgcgcgagtt ccgcttcggc
      601 gacagagcgc gcctgcgccc cttcgtcgag atcgacaacg tgccgaacaa gcgggtcttc
      661 accttcggag cggagttcat caacttcgac gccttgcgcg ccgacgcgac cgaagagcag
      721 cgccgcgcct tcgccgacac cttcctggtg ccgacgcgca cgctgcgccc acggacggtg
      781 cgcctgggcg tcagattcga tttctgaagt agggtcggtg aaggttagat gtcaacagaa
      841 cccggactat aatcggcccc tggtgcgtct tgccaaagaa aacggattcc ggagtgactt
      901 tgggcagagt gtttcgggca aggtaaaggg gcggcaggac tgacttgccg cccctcatct
      961 cacgatcttg cgtagtgggg tcgcatcaac ggcctcactc cgccgatgca gcggcgctcg
     1021 cgttcagctc tccccggatg gccgcgcctt atatcaccgg taagtgtttc gggcaaaggt
     1081 ctcaatacgt ccgctttatc ctgcccgtta ttgttttcaa ttgacaggaa tggaaaatac
     1141 caatactctt cgcctgcttt tgccacgtct actccgccct gttcgccgca gtaccctaca
     1201 agtaacccgg cgttggttgt ctgtcacaat aaccgagtgg ccgggattgc gacggacttg
     1261 gccgcggtga cctgattgaa gtctcgccgt cttctgactc gcataccctt gatgcccaaa
     1321 caatgcgagg ctccgggcac ccgccagtac cccaacggat gtgatcttat gtacggtcag
     1381 ggtctcgcca ccaacctttt aactgggcgg ggttgacgac acaaattgat tccgaaggag
     1441 cgcacacaat gcctgaagac acaccggata gcggagccaa agtaaccctc gacgctgaca
     1501 cggccagcaa gatggagtcg gtgttgcgcg acgggttgag cgggtcgcag gcctacatcg
     1561 ctgatgacga gccggacgac caagggaaga agaaagcagc gtccgtcgag cttgacgcgc
     1621 cgaccgtcgc gcaattcgcc gaccatctca aggagaggct ctcggcgtcg ggggcgtaca
     1681 tcgccgagga cgacgcgggc gaaggcgccg agaagaagta gcaggggacg acgaaaaacg
     1741 aggggtgcgg gcgaatggcc gcgggcgccg agcgcgcgtg ggtccagtat gggtcagact
     1801 tttcgactca gttcgagtct ccgcgccgag ccgctcgaag tgacactggc gcgagcccgc
     1861 gccgtcgccg ggtggctggg cattacccgc gtcaccgaga tcactcgtct cgacagggtc
     1921 ggcgtacccg tgttcgccag tatccgaccg aacgcattgc ccggctcgct gtgcgtcaac
     1981 gcgggcaagg gcgtgtcagt catggaggct cgggtcgggg cgtacatgga agccgtcgag
     2041 ttcgccttcg ccgagtacgg gcggtcgggg cttgaagtgc acgcggcgcc ggcgcggcag
     2101 gtatacgagg gacccgaccg tcccgacgcc atccttgatt tttgtccggt gatgcgggcc
     2161 gaggtcgccc tcgacgcgac ggtggcgtgt gtagaggccg ccgacatcgt gagcggagaa
     2221 aaatttccgg tccccgccga gctggttttt ctgccgttcc ccggagcgct gcgaactccg
     2281 cgctacttca gctcgaactc gaacggtctg tgttccggca actcggtcct tgaggcgacc
     2341 gtccacgggc tggccgaggt catcgagcgc gattgcatct ccttcatgct cgtggacgat
     2401 cagtcccgtc tcgtcgcggc gcccaccctg ccgccgcccc tgcgcgacct cgcggcgcag
     2461 gtgtccgccg ccggtctcga actctacctc cgacaccaga gcaatgtctt tggcgtcccc
     2521 ttcttccggg cggtcgtcgc cgagcagggc tgcacggacc cggtctacat ctcggagggc
     2581 tacggctgcc atctctccag agacatcgcc gcgacgcgcg ccgtctgcga gtcacttcag
     2641 agccgcctgt cttttatcca cggcggacgg gacgacctga ttaagcgcta caagttgttc
     2701 ggctcgcgcg cggagaatga gcgcgcggcc tacgccgagc ggttactggg ggaagtgtcc
     2761 gggagcgcgg gggccattga tttcgaggaa acagcggatt gctcggacgt ggcgacggac
     2821 ctggcctcgg ccctggcggt gttgacggcg aagctggcgc aaaacggaat acggcgcgtg
     2881 ctgagggtca tctacacgcc ggcagattac cccgtgcacg tggcgcgcat cctggcgccg
     2941 gggcttgaat gctttaacca tacgacggcc cgcgtcggcc tgcgcctgag agatcatgtc
     3001 agatcgcgtc tctaaccgtt tcatgttcgc cgggccgagc ttctgcggcg aagagcggct
     3061 gacggcggag gccggcggaa tgatgcgcgt cctgccgccg gtgcggcgcg gcgacgtgga
     3121 gagcctcgtc tccgcgcacc cgccgggcgt gctcgtgttg gttgacggta actttcacca
     3181 gtgtttgtcc gttgggcacg cggagattcg cgcggcgatt gaggccggct ggtccgtgtg
     3241 gggtctgtcc tcgatggggg cgatacgcgc ttacgagatg cggcacctgg ggatgaaggg
     3301 atacgggagg gtctaccgtt gcttttacga gcatgaagac ttccgcgacg acgaggtcgc
     3361 gctcgtccac gagccggtgc cgccctacca cgccgtcagc gagccgctcg tccacatccg
     3421 cttcttcctc cgcgaactcg tcagccggcg cctgctcacc gaaccctccg aaagggccat
     3481 ccttgagacg ttcatgtcgc tgtggttcgg cgaccggaca ctcccccttc tccgctccat
     3541 cctgcttgaa acgaccccgc gacacgaaga ggaagtcgcg gacttgctcg ccaatttccg
     3601 acgcttccgc gtcaaatgcc gcgacctggc cgactttttg cgagagcggc cctggggggc
     3661 ctcgcggcgg gcgggttacg aggagagacc ggtcaaccgc agcagttcga cgacccgcga
     3721 atcgcccaac aggtgatcga agcagggttc cgtcccgagc tgcaggagca gccgctcacg
     3781 ttcttcgtaa gcccgccgta accattcgat ggcagagtcg tcatcgccga ggtgcgcata
     3841 tgaggtcgcc agtgcgtatg gcgagacgcc gtcgccgcgc tcccggtctt gcttcaacag
     3901 ctcgcaactc tttcgccaga agccccgctc gccgcgcgag gcgtgagcct ctttgagcga
     3961 ggcggccctg cgcgcggcct gccccgcgtc tttacctgcc agaacatccg ccctctcgaa
     4021 a
//
LOCUS       DBNGOH010000892         4728 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_318792, whole genome shotgun sequence.
ACCESSION   DBNGOH010000892 DBNGOH010000000
VERSION     DBNGOH010000892.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4728)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4728)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4728
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_318792"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..705
                     /gene="panC"
                     /locus_tag="AC159P_17310"
     CDS             <1..705
                     /gene="panC"
                     /locus_tag="AC159P_17310"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HIR3697256.1"
                     /translation="TQFGPGEDFARYPRDLTQDTTRLADYNVDYIFAPAVEEIYPKNF
                     STYITVEGLSDPMEGAARPGHFRGVATVLTVLFNTVRPDFAFFGQKDAQQTLVVKRLV
                     RDLAFDIEVVVLPTVREQTGLALSSRNAYLSADDRRAAAVLYRALSQAREVYNEGERA
                     PKRLAETVRAQIETEPRARLEYVGVVDAETMEKLDRVPEDRAVLIALAARVGGTRLID
                     NIVLQPARQKTRGTEA"
     gene            1324..3111
                     /locus_tag="AC159P_17315"
     CDS             1324..3111
                     /locus_tag="AC159P_17315"
                     /inference="COORDINATES: protein motif:HMM:NF024758.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HIR3697257.1"
                     /translation="MERIVLRHLSGSKANQVEEFPLNHVKELILGRDPSATVRYDPDR
                     DDLVGRQHARVAQDPNDPTVFTVTDLNSRNGTFVNRQRISGTTRIGPGDVVQLGPGGP
                     EFQFDLEPRPAGGVKATRMAVSGTAATLPGGGQPSAPQTRAAGTMPPTGAAPVPPTGQ
                     AASGAGAPGKVGKATVERMINENIAVTKKQQGRKFLFIGGAAFVAVLLLFGAVAGFLG
                     WRYWKQQQVITEQKQEIESKIAGVETKVAEEAASKPMTWAQVAEDYGKAVVYIECSWR
                     LINTSNQAQVYHQFIANRGANNKPLVPGAPPILAAYVAVGEGYEPLLTYKNEPGTRPI
                     GGSHAGTGFVVSSNGLILTNRHVAATWKTAYSFPADTPPGVVFALNGQLLTRDGAPVL
                     VGPPTDWVPVNTKQEGRQIQGSFNGVNDVLDITFPKKENRIAARLVQSSDRHDIATIK
                     MDSLDPLTKVELNDNYDSVKIGEPVAIMGYPGAAPRSYGKIESKDVFNRETQYKSIPN
                     PTLTPTAIGAIHREQEAGGGKDAIINQIGDAYQLSTASTGAGNSGGPVFDDKGRVVAI
                     FFAGHRAGTTDAAITYAVPIRFAKQLMSN"
     gene            3202..4572
                     /locus_tag="AC159P_17320"
     CDS             3202..4572
                     /locus_tag="AC159P_17320"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HIR3697258.1"
                     /translation="MNRPFQQSSIIGEYRVVDYLGEGGMGQVYRAVHLKINRVVAIKA
                     LTSAGHNLGSIERFLNEACIQARLQHQNIATLYDFVECEGQPCIIMEYVDGQTLAERI
                     QELGRLPLHEALYVLQKIGDALAYIHENGIVHRDIKSNNVKITSRGQVKLLDFGIARA
                     ESGQTMTQVGMVIGTPIYLSPEQLRGERADARSDIWALGVLFYEMLTGDMPFKAETFG
                     ELYERITAGGYPPPASLNRAVSRGAEAVIARCLKKNAAERYQTVDELLSAVRSLGASA
                     PTVTPDGPHDSQPPDKRPPDKRPPDNDEGAAAPPPPPSWARRNWLGLTAGGAVAAVAL
                     FVVAGFGLFIYMDTPANNNAKTPPAATRPAPTQSTFGPNAAGAPATRPVHITTTDGKP
                     AEVYEIDRGTRELRHLGQTPYNYEGRFGDKVKLVLKREGFTDKPIEFDVRDSDQPWTV
                     TLETKK"
BASE COUNT          856 a         1726 c         1501 g          645 t
ORIGIN      
        1 acgcagttcg ggccgggcga ggacttcgcg cgctacccgc gcgacctgac gcaggacacg
       61 acgcggctcg ccgactacaa cgtggactac atcttcgccc cggcggtcga ggagatttac
      121 ccgaagaact tttccaccta catcaccgtc gagggcctgt ccgacccgat ggagggcgcg
      181 gcccgccccg gccacttccg cggcgtggcg accgtgctca ccgtcctctt caacaccgtc
      241 aggcccgact tcgccttctt cggccagaag gacgcgcagc agactttggt cgtcaagcgc
      301 ctcgtgcgcg acctcgcctt cgacatcgag gtcgtcgtgc tccccacggt gcgcgaacag
      361 acgggcctcg cgctctcgtc gcgcaacgcc tatctttcgg cggacgaccg ccgcgccgcc
      421 gccgtcctct accgcgcgct ctcgcaggcg cgcgaggtct acaacgaggg cgagcgcgcc
      481 ccgaagcgcc tcgccgagac ggtgcgcgcg cagatcgaga ccgagccgcg cgcccgcctc
      541 gaatacgtcg gcgtcgtgga cgccgagacg atggagaagc tcgaccgcgt cccggaagac
      601 cgcgccgtcc tcatcgcgct cgccgcccgc gtcggcggca cccgcctcat cgacaacatc
      661 gtcctccagc ccgcccggca gaagacgcgc ggcacggagg cttgaggccg tcccgtctcc
      721 gcgcccgccg ccctccggtc gcctgtccat cccgctctcg ggcgcgccgg gctgtcccgc
      781 tctgccgcga cccggaagcg ggccgtgggc acacgcgtca aaccgtcctg cttcgccact
      841 ccgtaatgtc cgcggcctcc atggcggaat gtccgcgcgc ggacatccgg gaacgaagag
      901 cgcgcggcca ccctttggtc ttccgcgcgg cgggcctttt atgtataatc ccgcctccaa
      961 aacctgcccg aggccggagc acagccggcc cggactgagc cggcgccgag cagcccccgg
     1021 tgcaatcgag cttggcggca gatgccagag agtttcggaa gttgacgcgc gtattcgacc
     1081 cgccggggaa tataaccttg aaacaggacg cgacttttcc gccgcacacg cgctcgcgcc
     1141 taagaagcga cggcgcctcg ctttgacgag tcctgaagcc tgtaccagta acgccgggaa
     1201 gcgatctccc ctcaaagagg ttgccccgcg acgccccctt cgcgtgctgc cgacgcggcc
     1261 cgtgaccgga ggggtaggcc gcgtcggccc gccccgcttg aacctgaccc cgaggagact
     1321 ttcatggagc gcatagttct caggcacttg agcggctcga aggccaatca ggtcgaggag
     1381 ttccccctca atcacgtcaa agagctgatt ctggggcgcg acccttcggc gaccgtccgc
     1441 tacgacccgg atcgtgacga ccttgtcggg cgtcagcacg cacgcgtcgc gcaagacccg
     1501 aacgacccca cggtcttcac cgtcaccgac ctcaacagcc gcaacggcac cttcgtcaac
     1561 cgccagcgca tcagcggcac gacgcgcatc gggccgggcg acgtggtgca gctcgggccg
     1621 ggcggccccg agttccagtt cgaccttgag ccgcggcccg cgggcggcgt caaggccacg
     1681 cgcatggccg tcagcggcac cgccgccacg ctccccggcg gcgggcagcc ctccgccccg
     1741 cagacgcgcg ccgccggcac gatgcccccg accggcgccg cgcccgtccc gcccaccggg
     1801 caggccgcct ccggggcggg cgcccccggc aaggtcggca aggccacggt cgagcggatg
     1861 atcaacgaga acatcgccgt cacgaagaag cagcaggggc gcaagttcct cttcatcggc
     1921 ggggcggcgt tcgtcgccgt gctgttgctg ttcggcgcgg tcgcgggctt cctcggctgg
     1981 cgctactgga aacagcagca ggtcatcacg gagcagaaac aggagatcga gagcaagatc
     2041 gccggcgtcg agacgaaggt cgccgaggag gccgcgtcga agccgatgac gtgggcgcag
     2101 gtcgccgagg actacggcaa ggccgtggtc tacatcgagt gctcgtggcg gctcatcaac
     2161 acgtcgaatc aggcgcaggt ctaccaccag ttcatcgcca accgcggcgc gaacaacaag
     2221 ccgctcgtgc cgggcgcccc cccgatcctc gcggcctacg tcgccgtcgg cgagggctac
     2281 gagccgctgc tcacctacaa gaacgaaccc ggcacgcgcc ccatcggcgg ctcgcacgcg
     2341 ggcaccggct tcgtcgtctc cagtaacggc ctgatcctca cgaaccgcca cgtcgccgcg
     2401 acgtggaaga ccgcctacag cttccccgcg gacacgccgc ccggcgtcgt cttcgccctg
     2461 aacgggcaac tgctgacgcg cgatggcgcc cccgtgctcg tcgggccgcc cacggactgg
     2521 gtgccggtca acaccaagca ggaggggcgc cagattcagg gcagcttcaa cggcgtgaac
     2581 gacgtgctcg acatcacctt ccccaagaag gagaaccgca tcgccgcgcg cctcgtccag
     2641 tccagcgacc gccacgacat cgccaccatc aagatggact cgctcgaccc gctgacgaag
     2701 gtcgagctga acgacaacta cgactcggtc aagatcggcg agccggtcgc catcatgggc
     2761 taccccggcg ccgcgccgcg ctcctacggc aagatcgagt cgaaggatgt cttcaaccgc
     2821 gagacgcagt acaagtccat cccgaacccg acgctgacgc cgaccgccat cggcgccatc
     2881 caccgcgagc aggaggccgg cggcggcaag gacgcgatca tcaaccagat cggcgacgcc
     2941 taccagctct cgaccgcctc gaccggcgcg ggcaactcgg gcggccccgt cttcgacgac
     3001 aaggggcgcg tcgtcgccat cttcttcgcc ggccaccggg ccgggacgac cgacgcggcc
     3061 atcacctacg ccgtcccgat ccgcttcgcc aagcagttga tgtcgaactg aaaaaaggac
     3121 tgaggactga gtgctgagga ctgagttaaa aacagatggc tttcactcag tcctcagcac
     3181 tcagtcctca gtcctgttga tatgaacagg ccgttccagc aaagctccat catcggcgaa
     3241 taccgcgtgg tcgattacct cggcgagggc gggatggggc aggtgtatcg agccgttcac
     3301 ttgaagatca accgcgtggt ggcgatcaag gcgctgacga gcgcgggcca caacctcggc
     3361 tccatcgagc gcttcctcaa cgaggcgtgc atccaggcgc ggcttcagca ccagaacatc
     3421 gctacgctct acgacttcgt cgagtgcgag ggccagccct gcatcatcat ggagtacgtg
     3481 gacggccaga cgctcgccga gcgcatccaa gagctgggcc ggctcccgct ccacgaggcg
     3541 ctgtacgtgc tccagaagat cggggacgcc ctcgcctaca tccacgagaa cggcatcgtc
     3601 caccgcgaca tcaagtcgaa caacgtcaag atcacctcgc gcgggcaggt caagctgctg
     3661 gatttcggca tcgcccgcgc cgagtccggc cagaccatga cgcaggtcgg catggtcatc
     3721 ggcacgccca tctacctctc gcccgagcaa ctgcgcggcg agcgggccga cgcgcgctcg
     3781 gacatctggg cgctcggcgt cctcttctac gagatgctga cgggcgacat gccgttcaag
     3841 gccgagacct tcggcgagct gtacgagcgc atcacggcgg gcggttaccc gccgcccgcc
     3901 tcgctcaacc gtgcggtgtc gcgcggcgcc gaggccgtca tcgcgcgctg cctgaagaag
     3961 aacgcggcgg aacgttacca gaccgtggac gaactgctct ccgccgtgcg gagcctgggc
     4021 gcgtcggcgc cgaccgtcac gccggacggc ccgcacgact cccagccgcc ggacaagagg
     4081 ccgccggaca agaggccgcc ggacaacgac gaaggcgcgg cggcgccgcc gccgccgcct
     4141 tcgtgggcgc ggcgcaactg gctcgggctg acggcaggcg gcgcggtcgc cgcggtcgcc
     4201 ctcttcgtcg tcgcgggctt cggcctcttc atctacatgg acacgcccgc gaacaacaac
     4261 gcgaagacgc cgcccgccgc cacccggccc gcgcccacgc aatcgacttt cggccccaac
     4321 gccgcgggcg ccccggcgac acggcccgtc catatcacca cgaccgacgg caagcccgcc
     4381 gaggtctacg agatcgaccg cggcacgcgc gaactgcgtc acctcggcca gacgccttac
     4441 aattacgagg ggcgcttcgg cgacaaggtg aagctggtgc tcaagcgcga gggcttcacc
     4501 gacaagccca tcgagttcga cgtgcgcgac agcgaccagc cgtggaccgt cacgttggag
     4561 acgaagaagt gagatgtgaa aggccgagga tggaaggcaa aacccgaggg gataaagggg
     4621 ccgaactacc ggaggaagcg attaaattca tgatgggtct attcggaaaa aagaagggca
     4681 agccggcgga cggcgacggc accgcgcggc cctcgttcca aatcgagg
//
LOCUS       DBNGOH010000893         7253 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1601117, whole genome shotgun sequence.
ACCESSION   DBNGOH010000893 DBNGOH010000000
VERSION     DBNGOH010000893.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7253)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7253)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7253
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1601117"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            88..759
                     /locus_tag="AC159P_17325"
     CDS             88..759
                     /locus_tag="AC159P_17325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007220976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697259.1"
                     /translation="MAYLLKGALIEYGSDFLGPLPNVVLFQFNPEKLTRNIQVPPRPV
                     GGANRETSQAGEPTIEKITLQASFSAADQLNTNDKLARVMGVGPRLAALEKMVHPAGK
                     LSGLIGAAIDAVGDALGLGGGDGARQPVPREAYPRILFVWGPFRLLPVVIESMNIVEL
                     QYDFLLNPTQADVNISLNVDPIDPCSDDLLGKGALAYSNLAKEAQAIANLANTAGQVV
                     ELIPF"
     gene            782..1054
                     /locus_tag="AC159P_17330"
     CDS             782..1054
                     /locus_tag="AC159P_17330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697260.1"
                     /translation="MFADSSRYFQLKTVDVTTPSGRQVKAVVLRRLPFVAGTPTTVKG
                     NDRLDVMAERNYRDATLFWHVADANTELDARELVAEVGRVILVPDK"
     gene            1074..2243
                     /locus_tag="AC159P_17335"
     CDS             1074..2243
                     /locus_tag="AC159P_17335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697261.1"
                     /translation="MATIEYRMFFGNEPADRKRLDLFEEISVSQQVDVMWEARLKMPV
                     VMDEKGKWKNEDEPFLQPYARVRVEVKVGDAAFVPLIDGPITEVVAPRNPHPGKSDVT
                     LVVHDDSVFLDQEEKLERFEDMTDSEIAERVFSEIPQLGGPPQVEPTPPQPDNPTRVV
                     VQRGTKIQVLRALARRHHFHAYVLPGAVAGESVGAFRTFPTATDGLPALTLAGEGRNL
                     ERFDPRTDARRPADVRAATLSLRDKRITTSRSGQSDATLQGDRPAVDAGADRPSRLLP
                     PGHSDTVDLDRLTRGEAERLSLATEVTGSVRNFCYKGVLTPYRLVPVKLSDSKLSGKY
                     LVTKVTHTLNRSLYKQSFTLRGNAFTEAAGTAGAPASPTASAALAAAFNTQGSVF"
     gene            2243..2866
                     /locus_tag="AC159P_17340"
     CDS             2243..2866
                     /locus_tag="AC159P_17340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007916676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HIR3697262.1"
                     /translation="MPTEEMENLVVDLAERMRDRFYGKYRGLVTNVDDPDNLGRITAQ
                     VPEVFEEEETPWAMPVVPFAGSGHGLVLLPEVGDGVWIEFESGDPSRPLWTGCWWGDG
                     EMPDPGGTRTRVLATTAGHKLVLDDAGDEVKLLHAGGAELKMTGGDITLSIGQAELKM
                     TATGISLSIGASEIKLSPADITIKSGGAQIKLAGVVVNVNNGAITVI"
     gene            2937..3281
                     /locus_tag="AC159P_17345"
     CDS             2937..3281
                     /locus_tag="AC159P_17345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697263.1"
                     /translation="MPGSVLTAASTVLCMHGGRAILTTTNTRLLVGGVPALLQTDVHA
                     VAGCPFQIPFGVGTKPSPCIRIEWQGGSTKLAIGGVGVLVQTSIGICYSPEGAPQGVA
                     IVAATAPGTSSG"
     gene            3347..3727
                     /locus_tag="AC159P_17350"
     CDS             3347..3727
                     /locus_tag="AC159P_17350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017296923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HIR3697264.1"
                     /translation="MKPENGRHLSFPFRVGRDGRAAQISTLEEHVRDELVQLLLTNPA
                     ERLFLPDFGGGVRRLVFESADDATAGLAKAVITQSVTRYLGHRVTLEELTVVAAGATI
                     EIDLKYRIAGTEDTRVLRFQRRGG"
     gene            3808..6594
                     /locus_tag="AC159P_17355"
     CDS             3808..6594
                     /locus_tag="AC159P_17355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017296924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HIR3697265.1"
                     /translation="MSIINREALEERARKLFTVPTEISDDLFGALSLACRTGTLPPFY
                     SQTAPGDPHVLIPKPHELSDVQRNDILECLGVLNGIKSVEVRLVAGVAELDLRFFNRN
                     SLNTIFTAISGDPALAKSVFPLTGGHRLPAGQAEGQVQTVAVNAGAVEASLVLTIAPV
                     GDYSTYTLAVDTSAVAALPNPTFDPLFDRIEFKFRPGCFNTNCAPEWKKPAPPKPAPA
                     IDYLAKDYDSFRHAMIAAMMERVPGWEPSSEADLDQVLLELFSAAADELSDYQDRVMN
                     EAYLATARKRVSLARHARLVDYHIHQGNQASTWVALELIVVPPPAPADELFVREGFRF
                     WAGGEEESDASAVVFMTRARQPVHPLANRLGLYTWSNAVPSLAAGSTTADIEILVGTE
                     EAPAPLPDSAREAAAKQLRDMIRAGTISRLLVEERLNPATGGRAGRDPQRRQLLRLVG
                     GDAGAEAMFDPATGTSATARWFVRVRWREVDALRRNYCFTVECPARVEHVSFFHGNLV
                     EAHHGRPADNVFKESSALLAAPNEFHYERLLGPASPADDEPWLDAPETPAPEQPWSVI
                     CRLPAGPLAYRNTPPGGDIAPQSTLAVTVVTASDSDAWDEVPNLIHSDASDENGDHFV
                     VETDELGRSLVRFGNGINGKLLPADAVVRCAYQVGEGLEGNIGSDRLVNFDRAASAII
                     KRSLGVETQLLPAQGVLLKTWNPFDVTNGRAPEPAAEIIRRAPEAYRLRQLRAVTLRD
                     YVERAEAVEGVSRAAASYAWTGSWRTVRVTIDAAGTGVLTDALRRTVARQLEAVRLIG
                     EDLEIRPPRFVPLDIRVALCVAPDYWPEDLRFLLEQEFSDGYTPDGRRAFFHPDLWTF
                     GQPLRASQIIGRAQSVQGVEHVISVRIKRWNETGTGTDRIVSLRHNEIIRVANGPDHL
                     ERGFIFFDLKGGRQ"
     gene            6591..>7253
                     /locus_tag="AC159P_17360"
     CDS             6591..>7253
                     /locus_tag="AC159P_17360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017296925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697266.1"
                     /translation="MSEDCRNDCVAPLDFPRRIFNRPGLARIGYRVGSFADIREALLR
                     DLDHDAVLAAWTHRGADDPGIALLEGASILGDILTFYQELYANEAYLRTAQWRESISD
                     LVRLLGYRLSPGLGGNATFAFEVKGDRAVTVPAAFPVKAQVEGLDKPADFETTDELLA
                     YPWLSRFSLFRPLVWPNLTPQTTEFYIKSPDQTTAPVELEEGDRLLVGDSVAGSPGRI
                     DNA"
BASE COUNT         1240 a         2632 c         2350 g         1031 t
ORIGIN      
        1 acaagccctg cccctacagt catcacgaac gctgagcagt ttgcaacaga ctctgaggag
       61 acgaggggaa aaggagttta gcgaatcatg gcttacctac tgaaaggcgc gctcatcgaa
      121 tacggcagcg atttcctcgg gccgctgccg aacgtcgtgc tcttccagtt caaccccgag
      181 aagctgacgc gcaacatcca ggttccgccg cgcccggtcg gcggcgcgaa ccgcgaaacg
      241 tcgcaggcgg gcgagccgac catcgagaag atcaccttgc aggcgagctt cagcgccgcc
      301 gaccagctca acacgaacga caagctcgcg cgcgtgatgg gcgtcggccc gcgcctcgcc
      361 gcgctcgaaa agatggtgca cccggcgggc aagctctcgg gcctcatcgg cgcggcgata
      421 gacgcggtcg gcgacgcgct cggtctcggc ggcggcgacg gcgcgcgcca gcccgtcccg
      481 cgcgaggcgt acccgcgcat cctcttcgtc tgggggccgt tccgcctgct ccccgtcgtc
      541 atcgagtcga tgaacatcgt cgagctgcaa tacgacttcc tgctcaaccc gacgcaggcc
      601 gacgtgaaca tcagcctcaa cgtcgatccc atcgacccgt gctcggacga cctgctcggc
      661 aagggcgcgc tcgcatactc gaacctcgcc aaagaggcgc aggccatcgc caacctcgcc
      721 aacaccgccg ggcaggtggt cgagttgatc ccgttctgag agagcgaagg agacgcggaa
      781 gatgttcgca gactcgtcga gatactttca gctcaagacc gtggacgtga cgactccttc
      841 gggccgtcag gtgaaggccg tcgtgctgcg ccgtctgcct ttcgtcgcgg gcacgccgac
      901 gaccgtcaag ggcaacgacc ggctcgacgt gatggccgag cgcaactaca gggacgcgac
      961 gctcttctgg cacgtcgccg acgccaacac cgaactcgac gcgcgcgaac tcgtcgccga
     1021 ggtcgggcgc gtcatcctcg tccccgacaa gtgaagcgga gtggaaggca ccgatggcga
     1081 ccatcgagta caggatgttc ttcgggaacg agccggctga ccgcaaacgg ctcgacctct
     1141 tcgaggagat cagcgtcagc cagcaggtgg acgtgatgtg ggaggcgcgt ctgaagatgc
     1201 cggtcgtgat ggacgagaag ggcaagtgga agaacgagga cgagccgttc ttgcagccgt
     1261 acgcgcgcgt gcgcgtcgag gtcaaggtcg gcgacgcggc cttcgtgccg ctcatcgacg
     1321 gccccatcac cgaagtcgtc gccccgcgca acccgcaccc cggcaagagc gacgtgacgc
     1381 tcgtcgtcca cgacgacagc gtctttctcg atcaggagga gaagctcgaa cgcttcgaag
     1441 acatgaccga cagcgagatc gccgaacgcg tcttctcgga aatcccgcag ctcggcgggc
     1501 cgccgcaggt cgagccgacg ccgccgcagc ccgacaatcc gacgcgcgtc gtcgtccagc
     1561 gcggcacgaa gattcaagtc ctgcgcgcgc tcgcgcgccg ccaccacttc cacgcctacg
     1621 tgctgcccgg cgccgtggcg ggcgagagcg tcggcgcctt ccgcaccttc ccgaccgcga
     1681 ccgacggcct gcccgcgctc acgctcgcgg gcgagggccg caacctcgaa cgcttcgacc
     1741 cgcgcaccga cgcgcgccgc ccggcggacg tgcgcgccgc caccttgagc ctgcgcgaca
     1801 agcgcatcac gacgagccgt tcgggccagt cggacgcgac gctgcaaggc gaccgtccgg
     1861 cggtcgatgc gggtgcggac aggccgtcgc gcctgctccc gcccggccac agcgacacgg
     1921 tcgatctcga ccggctgacg cgcggcgagg ccgagcgcct gagcctcgcc accgaggtta
     1981 cgggcagcgt ccgcaacttc tgttacaagg gcgtgctcac gccctaccgg ctcgtccccg
     2041 tcaagctctc cgacagcaag ctcagcggca agtacctcgt caccaaagtc acgcacaccc
     2101 tgaaccgctc gctctacaag cagtcgttca cgctgcgcgg caacgccttt accgaggccg
     2161 cgggcaccgc gggcgccccg gcctcgccga cggctagtgc ggcgctcgcc gccgccttca
     2221 acacgcaggg gagcgtcttc taatgcctac cgaagagatg gagaacctgg tcgtcgatct
     2281 ggccgagcgg atgcgcgacc gcttctacgg gaagtaccgc ggcctcgtca ccaacgtgga
     2341 cgaccccgac aacctcgggc gcattacggc gcaggtgccg gaggtcttcg aagaagagga
     2401 gacgccgtgg gcgatgccgg tcgtgccctt cgcgggcagc ggtcacgggc tggtgctgtt
     2461 gcccgaggtg ggcgacggcg tgtggatcga gttcgagtcg ggcgaccctt cgcgcccgct
     2521 ctggacgggc tgctggtggg gcgacggcga gatgcccgac cccggcggca cgcggacgcg
     2581 cgtgctggcg acgaccgccg ggcacaagct cgtgctcgac gacgcgggcg acgaggtcaa
     2641 actcctgcac gcgggcggcg ccgagctgaa gatgacgggc ggcgacatca cgctctccat
     2701 cgggcaggcg gagctgaaga tgacggccac cggcatctcg ctctccatcg gcgcgagcga
     2761 gatcaagctg agtcccgccg acatcacgat caagagcggc ggcgcgcaaa tcaaactcgc
     2821 cggcgtggtc gtcaacgtca acaacggcgc gatcacagtg atttgacgcc gcgatgaacg
     2881 tgaggtcggc ggcgcgaccg gcgtgatgtg aacggtacga gaggcgagga ggcgagatgc
     2941 ccggaagcgt tctgacggcg gcgagcaccg tgctgtgcat gcacggcggg cgggccatcc
     3001 tgacgacgac gaatacgcgg ctgctcgtcg gcggcgtacc ggcgctgctc cagaccgacg
     3061 tgcacgcggt cgcgggctgc cccttccaga ttcctttcgg cgtcggcacg aagccctcgc
     3121 cgtgcatccg catcgagtgg cagggcggct cgacgaagct cgccatcggc ggcgtcggcg
     3181 tgctggtgca gacgagcatc ggcatctgct acagcccaga gggcgcaccg cagggcgtcg
     3241 ccatcgtcgc cgccacggcg cccggcacga gttcgggttg accggacgag aaccgtcgaa
     3301 gcgcgaccgt cttgcgaaac gatgaagacc gacgacactt gagacgatga aacctgagaa
     3361 cggcagacat ctttcgtttc ccttccgcgt cgggcgcgac ggtcgcgccg cgcaaatctc
     3421 gacgctcgaa gagcacgtgc gcgacgagct ggtgcaactg ctgttgacca atcccgccga
     3481 gcggctgttc ctgccagact tcggcggcgg cgtccggcgg ctcgtcttcg agagcgccga
     3541 cgacgcgacc gcagggctgg caaaggccgt catcacgcag tccgtcacgc gctacctcgg
     3601 ccaccgcgta acgctcgaag aactgacggt cgtcgccgcg ggcgcgacca tcgagatcga
     3661 cctcaagtat cgcatcgccg ggacggaaga cacgcgcgtc ctgaggttcc agcggcgcgg
     3721 cggttagcgt agaggagggg agtgcggcgg cgcaagagct ttgagcgacg ccgcccgagt
     3781 ctcgcgagag cggagtggag tgggcctgtg tcgatcatca accgggaagc gctcgaagag
     3841 cgagccagaa aactcttcac cgtgccgact gagatcagcg acgacctctt cggcgcgctg
     3901 agcctcgcct gccggacggg cacgctgccc cccttctact cgcagaccgc gccgggcgac
     3961 ccgcacgtgc tcatcccgaa gccgcacgag ctgtcggacg tgcagcgcaa cgacatcctc
     4021 gaatgcctcg gcgtgctcaa cggcatcaag tccgtcgagg tcaggctcgt cgccggcgtg
     4081 gccgaactcg acctccgctt cttcaaccgg aacagcctca acaccatctt cacggccatc
     4141 tccggcgacc ccgcgttggc caagagcgtc ttcccgctga cgggcggcca ccgcctgccc
     4201 gccggtcagg ccgaggggca ggtacagacg gtcgccgtca acgcgggggc cgtcgaggcg
     4261 tctctcgtcc tcaccatcgc gcccgtcggc gactactcga cctacacgct cgccgtggac
     4321 acgagcgccg tggccgcact cccgaacccg accttcgacc cgctcttcga ccggatcgag
     4381 ttcaagttcc ggcccggctg cttcaacacc aactgcgcgc ccgagtggaa aaagcccgcg
     4441 ccgcccaagc ccgcgcccgc catcgactat ctcgccaaag actacgactc gttccgccac
     4501 gcgatgatcg cggcgatgat ggagcgcgtg cccggctggg agccttcgag tgaggccgac
     4561 ctcgatcaag tcctgctcga actgttcagc gccgccgccg acgagctgag cgactatcag
     4621 gatcgcgtga tgaacgaggc gtacttggcg acggcgcgca agcgcgtctc gctcgcacgc
     4681 cacgcgcgcc tcgtggacta ccacatccat cagggcaatc aggcgagcac gtgggtcgcg
     4741 ctcgagctga tcgtcgtgcc gccgcccgcg cccgccgacg aactgttcgt gagagagggc
     4801 ttccgcttct gggcgggcgg cgaggaagag tcggacgcct ccgcggtcgt cttcatgacg
     4861 cgcgcgcggc agcccgtcca cccgctcgcc aaccgcctcg gactctacac gtggtcgaac
     4921 gccgtgccgt cgctcgcggc gggcagcacc accgccgaca tcgaaatcct cgtcggcacc
     4981 gaggaggcgc ccgcgccgct cccagactcc gcgcgcgagg cggcggccaa gcaactgcgc
     5041 gacatgattc gcgcgggcac catcagccgc ctcctcgtcg aggagcgact caaccccgcg
     5101 acgggcgggc gcgcgggccg cgacccgcag cgcagacaac tgctccgcct cgtcggcggc
     5161 gacgcagggg ccgaggcgat gttcgaccca gcgaccggca cttcggccac ggcccgctgg
     5221 ttcgtgcgtg tgcgctggcg cgaagtggac gcgctgcgcc gcaactactg cttcacggtc
     5281 gagtgccccg cgcgcgtcga gcacgtctcg ttctttcacg gcaacctcgt cgaggcgcac
     5341 cacggcaggc ccgcggacaa cgtcttcaag gagtcgagcg cgctcctcgc ggcccccaac
     5401 gagtttcact acgagcggct gctcggcccc gcttcgcccg ccgacgacga gccgtggctc
     5461 gacgcccccg agacgcccgc gcctgagcag ccgtggagcg tcatctgccg cctgcccgcg
     5521 gggccgctcg cctaccgcaa cacgccgccg ggtggcgaca tcgcgccgca atcgacgctc
     5581 gccgtcacgg tcgtcaccgc gagcgactcg gacgcgtggg acgaagtgcc gaacctcatc
     5641 cacagcgacg cgagcgacga gaacggcgac catttcgtcg tcgagaccga cgagcttggt
     5701 cgcagcctcg tccgtttcgg caacggcatc aacggcaagc tcctgcccgc ggacgccgtc
     5761 gtgcgctgcg cttatcaggt cggcgaagga ctcgaaggca acatcggctc cgaccgtctg
     5821 gttaacttcg accgtgcggc atcggccatc atcaagcgct ccctcggcgt ggagacgcag
     5881 cttttgcccg cgcaaggcgt gctgctgaaa acatggaacc ccttcgacgt gacgaacggg
     5941 cgcgcgcccg agccggcagc cgagatcatc cgccgcgcgc ccgaggctta ccgcctgcgg
     6001 cagcttcgcg ccgtcacgct gcgcgactac gtcgagcgcg cagaggccgt cgagggcgtc
     6061 tcgcgcgcgg cggccagcta cgcgtggacg gggagctggc gcaccgtccg cgtcaccatc
     6121 gacgcggcgg gcacgggcgt gctcaccgac gcgctgcgcc gcacggtcgc gcgccagctc
     6181 gaagccgtgc ggctcatcgg cgaagacctt gagattcgcc cgccgcgttt cgtcccgctc
     6241 gacatccgcg tcgcgctctg cgtcgcgccc gactactggc ccgaagacct gcgcttcctc
     6301 ctcgaacagg agttctccga cggctacacg cccgacggtc gacgcgcctt ctttcaccca
     6361 gacctctgga cgttcggcca gcccctgcgc gcgagccaga tcatcgggcg cgcgcagtct
     6421 gtgcagggcg tcgagcacgt catctccgtc aggatcaaac gttggaacga gaccggcacc
     6481 ggcaccgacc ggatcgtgag cctgcggcac aacgagatca tccgcgtcgc gaacggcccc
     6541 gaccacctcg agcgcggctt catcttcttc gacctgaagg ggggcagaca atgagcgaag
     6601 actgccgcaa cgactgcgtc gcccctttgg acttcccgcg cagaatcttc aaccgccccg
     6661 gcctcgcgcg catcggctac cgcgtcggct cgttcgccga catccgcgag gcgctgctgc
     6721 gcgacctcga ccacgacgcc gtgctcgccg cgtggacgca ccgcggcgcg gacgaccccg
     6781 gcatcgcgct cctcgaaggc gcctccatcc tcggcgacat cctgaccttc tatcaggagc
     6841 tttacgccaa cgaggcctac ctgcgcaccg cgcagtggcg cgaatcgatc tccgacctcg
     6901 tgcgcctgct aggctaccgc ctctcgcccg gcctcggcgg caacgccacc ttcgccttcg
     6961 aggtgaaggg cgaccgcgcc gtcacagtcc ccgccgcctt ccccgtcaag gcgcaggtcg
     7021 aaggactgga caagcccgcc gacttcgaga ccacggacga gctgctcgcc tacccctggc
     7081 tcagccgctt cagcctcttc cgcccgctcg tctggccgaa cctcacgccg cagaccaccg
     7141 agttctacat caagtcgccg gatcagacga cggcgcccgt cgaactcgaa gaaggcgacc
     7201 gcctgctcgt cggcgacagt gtggccggct cgcccggtcg catcgacaac gcc
//
LOCUS       DBNGOH010000894         3264 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2884074, whole genome shotgun sequence.
ACCESSION   DBNGOH010000894 DBNGOH010000000
VERSION     DBNGOH010000894.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3264)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3264)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3264
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2884074"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            39..1400
                     /locus_tag="AC159P_17365"
     CDS             39..1400
                     /locus_tag="AC159P_17365"
                     /inference="COORDINATES: protein motif:HMM:NF024753.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HIR3697267.1"
                     /translation="MKPSTRIALALVACLVTLAAVSVFNFNDAAASAADSNWPAWRGP
                     EGSGVSAEKNLPEEWAADKNVLWKTPVAGRGHSSPIVWGKRVFLTTAVEGEVVPGAKA
                     AVHMVNGKEWAHPESLGADRKHTFKVVALDRDSGKVLWETTAWEGTPYDNRHRKSSYA
                     ASTPATDGKLVFSFFGAEGLYAHDMNGKLAWKADLGKIGTGGMGTGTSPIVHENLVII
                     QADEEDGVNSYIAGFDKKTGKQVWKAPRKVQASWTTPLLVNTGKRTEMITLGNEFVIA
                     YDPANGKELWRSKGVDSNAIPSPVASREMVYVSAGYPVKTAFAIRLGGSGDLTGSKEI
                     VWTYAKGTAYVPSPIYYDGFFYLTTDRGILTCLDARTGEVKYEGGRIPVPATFTASPV
                     AYDGKLLLTSEDGDTFVIRAGPKHEVIRTNSIGEPVYASPAIADGKIFIRGEKHLYAI
                     GKR"
     gene            1613..3220
                     /locus_tag="AC159P_17370"
     CDS             1613..3220
                     /locus_tag="AC159P_17370"
                     /inference="COORDINATES: protein motif:HMM:NF024753.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HIR3697268.1"
                     /translation="MFEQVPESTAWYHSWPVVLAATVVFPPLGLVLLWTRRGTDMQLK
                     VLGSLAVVLLCAGYAYALLGPRRSANTEGHYTELERHREAQRQQPPPPEVAQATATAA
                     DPNAAAQASPGADAAAGQPPQPAAPGTEAAAASAPGQPAAPSSAAPPARRNYWTNFRG
                     PSRDGRYEEQTLKTNWGAGGPAKLWSQPVGGGYASFSVADGIAYTIEQRRGQETVAAY
                     HVDTGRELWTHGWNAEYRDSPGDGPRATPTWDAGRVYALGALGELRCLDAKTGKPVWS
                     KNILSDNGASNIQWAMAASPLIVDDKVVVMAGGRAGKSVVAYNKATGARAWGALGDEA
                     SYTSPMLVNLAGRRQILVVTANQAAGIAPEDGAVLWSYPWRNSAGINISQPIVAGPNR
                     VFISAGYGKGAALFEVASSGKAFNATTVWENVSMKNKFNSSVLHDGHVYGLDEGILSC
                     VDVNTGERKWKGGRYGYGQVVLAGGHLIITTEEGEVVLVRAAADKHTELARFKALDGK
                     TWNNPAIAGGRLLVRNAEQMACFKLTD"
BASE COUNT          601 a         1162 c         1050 g          451 t
ORIGIN      
        1 acgcacattt aatccagata acgacaaagg aaccgaacgt gaaaccgtca accagaatcg
       61 ccctcgccct cgtcgcgtgt ctcgtgacgc tcgccgccgt ctcggtcttt aactttaacg
      121 acgccgccgc gtccgccgcc gactcgaact ggcccgcgtg gcgcggcccc gaaggctcag
      181 gcgtctcggc ggagaagaac ctgccggagg agtgggccgc cgacaagaac gttctgtgga
      241 agacgcccgt cgccgggcgc ggccactctt cgcccatcgt ctggggcaag cgcgtgtttt
      301 tgacgacggc ggtcgaagga gaggtcgtcc ccggcgcgaa ggccgccgtg cacatggtca
      361 acggcaagga gtgggcgcac cccgagagcc tcggcgccga ccgcaagcac accttcaagg
      421 tcgtcgccct cgaccgcgac agcggcaagg tcttgtggga gacgacggcg tgggagggca
      481 cgccctacga caaccgccac cgcaagagtt cctacgcggc ctcgacgccc gcgaccgacg
      541 gcaagctggt cttctccttc ttcggcgccg agggtctcta cgcgcacgac atgaacggca
      601 agcttgcgtg gaaggcagac ctcggcaaga tcggcaccgg cggcatgggc acgggcacct
      661 cgcccatcgt ccacgaaaac ctcgtcatca tccaggcgga tgaagaggac ggcgtgaact
      721 cttacatcgc cggcttcgac aagaagacgg gcaagcaggt ctggaaggcg ccgcgcaagg
      781 tgcaggcgag ctggacgacg ccgctgctgg tcaacacggg caagcgcacc gagatgatca
      841 cgctcggcaa cgagttcgtt atcgcctatg acccggcgaa cggcaaagag ctgtggcgca
      901 gcaagggcgt ggacagcaac gccatcccct cgcccgtcgc ctcgcgcgag atggtctacg
      961 tctccgccgg gtatcccgtc aagacggcct tcgccatccg cctcggcggg agcggcgatt
     1021 tgaccggctc gaaggagatc gtctggacgt acgcgaaggg cacggcctac gtcccctcgc
     1081 cgatctacta cgacggcttc ttctacctga cgaccgaccg cggcatcctg acctgcctcg
     1141 acgcgcgcac cggcgaggtc aagtacgaag gcgggcgcat ccccgtcccc gccaccttca
     1201 ccgcctcgcc cgtcgcctac gacggcaagc tgctgctgac cagcgaggac ggcgacacct
     1261 tcgtcatcag ggccggaccc aaacacgagg tcatccgcac caactccatc ggcgagccgg
     1321 tctacgcctc gcccgccatc gccgacggca aaatcttcat ccgcggcgag aagcacctgt
     1381 acgccatcgg gaagcgatag gcataagggg gaaaaggtga aaaggggaag aaggtaaaag
     1441 ggaaggagca gcagagcgtg gctcacgctc taccttttcg ccccttcccc ctttaccctt
     1501 ttcccccttc cgctttcccc ttccaaaaat atggaacgcg tccgcggcct cgaacgtcta
     1561 aaatgggaag caggcttcaa ccccttcctc ttccgactgg gagcgacaga aaatgtttga
     1621 acaggttccg gagtcaaccg cgtggtatca ctcgtggccg gtcgtgctgg cggcgacggt
     1681 cgtcttcccg ccgctcggac tcgtgctgct gtggacgcgg cgcgggaccg acatgcagtt
     1741 gaaggtgctc ggctcgctcg ccgtcgtcct gctctgcgcg ggctacgcgt acgcgctctt
     1801 aggcccgcgg cgcagcgcca acaccgaggg ccactacacg gagcttgagc gccaccgcga
     1861 ggcgcagcgc cagcagcccc cgccgcccga ggtcgcacag gccacggcca ccgccgccga
     1921 cccgaacgcc gccgcgcaag cctcgcccgg cgccgacgcc gccgccgggc agccgccgca
     1981 gcccgcggcc cccggcacgg aggccgccgc cgcttcggcc cccggccaac ccgccgcgcc
     2041 ttcctccgcc gcgccgcccg cgcgccgcaa ctactggacg aacttccgcg gcccttcgcg
     2101 cgacggtcgc tacgaagagc agacgctcaa gaccaactgg ggcgcgggcg gccccgcgaa
     2161 actgtggagc cagccggtcg gcggcggcta cgcctcgttc tcggtcgcgg acggcatcgc
     2221 ctacaccatc gagcagcggc gcgggcagga gacggtcgcc gcctaccacg tcgatacggg
     2281 gcgcgagctg tggacgcacg gctggaacgc agagtacagg gactcgcccg gcgacggccc
     2341 gcgcgcgacg ccgacgtggg acgcgggacg cgtctacgcg ctcggcgcgc tcggcgagct
     2401 gcgctgcctc gacgccaaga caggcaagcc cgtctggtcg aaaaacatcc tcagcgataa
     2461 cggcgcctcg aacatccagt gggcgatggc cgcctcgccg ctgatcgttg acgacaaggt
     2521 ggtggtgatg gcgggcggtc gcgcgggcaa gtccgtggtc gcctacaaca aggcgacggg
     2581 cgcgcgcgcg tggggcgcac tcggcgacga ggcttcgtac acctcgccga tgctcgtcaa
     2641 cctcgcgggc cgccggcaga ttctcgtcgt caccgcgaac caggcggcgg gcatcgcgcc
     2701 cgaagacggc gccgtcctgt ggtcgtaccc gtggcgcaac tccgccggca tcaacatctc
     2761 gcagcccatc gtcgccggcc cgaaccgcgt cttcatctcc gccggctacg gcaagggcgc
     2821 cgcgctcttc gaagtcgcga gcagcggcaa ggcgttcaac gcgacgacgg tctgggagaa
     2881 cgtctcgatg aagaacaagt tcaacagctc ggtgctccac gacggccacg tctacggact
     2941 cgacgaaggc atcctctcgt gcgtggacgt gaacacgggc gagcgcaagt ggaagggcgg
     3001 tcgctacggc tacggccagg tcgtcttggc cggcggccac ctcatcatca cgaccgaaga
     3061 gggcgaggtc gtcctcgtcc gcgccgccgc cgacaagcac accgaactgg cgcgcttcaa
     3121 agccctcgac ggcaagacct ggaacaaccc cgccatcgcg ggcggtcgcc tcctcgtccg
     3181 caacgccgaa cagatggcct gcttcaagct caccgactga acgaagcgtg gaaggcaaaa
     3241 gtgcgagggg ccgagggagt gtca
//
LOCUS       DBNGOH010000895         7555 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_5084704, whole genome shotgun sequence.
ACCESSION   DBNGOH010000895 DBNGOH010000000
VERSION     DBNGOH010000895.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7555)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7555)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7555
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_5084704"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2218)
                     /locus_tag="AC159P_17375"
     CDS             complement(<1..2218)
                     /locus_tag="AC159P_17375"
                     /inference="COORDINATES: protein motif:HMM:NF053443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolB family protein"
                     /protein_id="HIR3697269.1"
                     /translation="MLRRFPVCLLMLSLLLNAQAWPSARNRAGGDSRAQDAPKKAEPE
                     AAKKFEPWDVEGDHGPSTLVEFDTDEGTWMSCDVSPDGQRLVFDLLGDIYVMPVAGGE
                     ARLVSGGASWEVQPRWSPDGKWIAFTSDRDGGDNIWVADAEGKNRRQVTKETTRLVNT
                     PAWTPDGQYLLARKHWTDTRSAGAGEIWMYHVNGGGKGVQLTEKPNWTANIGEPVVEP
                     KGRFMYFVASGAFDYNKNVYDSIYWIERYDLQKGRRSVFIRGAGGAIRPAISPDGKLL
                     SFIRRVGLKTVLFMREIETGREWPVYDQLTRDQQETWAIFGTYPGYAWTPDSKAVVIT
                     AQGKFVRVNVESRQATPIPFKAQVAQRVSEAVRSPVKVAAERERARLLRWAERNGDRI
                     IYSALGRIYVKEGEAAPRPLLNSKYLEYAPRFSADGRRIAYVTWRDDEKGAVWTANAD
                     GTNPVRITTAPDLYANPVFSRDGSKIAYLKGRGSVYHEENLASESSFEIHYWDGRAHS
                     YVMDLQSRGPNLRMPVLSFDPEGERIFFMEAGGGPAPTPTAPAQFITYLSSVKLGGDD
                     FKRHIEGAYVTEIIPSPDHKWIAFRELHKLYVAPFPQIGKTVKLSSTETNVPVRPASP
                     QAGNWLAWSPDSKSVQWTLGENFYEQSLENINKALGKDEKPPPPRATVIGFDFETARP
                     RGLVALLNARLVTMRGDEVIEKGTILVEDNRIKAVGARLQIPSGAKRIDMTGKTIAP"
     gene            2386..2763
                     /locus_tag="AC159P_17380"
                     /pseudo
     CDS             2386..2763
                     /locus_tag="AC159P_17380"
                     /inference="COORDINATES: protein motif:HMM:NF023075.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group I intron-associated PD-(D/E)XK
                     endonuclease"
     gene            complement(2809..2882)
                     /locus_tag="AC159P_17385"
     tRNA            complement(2809..2882)
                     /locus_tag="AC159P_17385"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2846..2848),aa:Arg,seq:ccg)
     gene            3046..4761
                     /locus_tag="AC159P_17390"
     CDS             3046..4761
                     /locus_tag="AC159P_17390"
                     /inference="COORDINATES: protein motif:HMM:NF016289.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HIR3697270.1"
                     /translation="MRNSKLYALALAVALLTLPVAPPRAQQARKATAAATKAAVPSAA
                     FAAARKDAEEITAAKMKEVLYYISSDAMAGRDTPSEGLDKTAEYIADRLAKLKLKPAG
                     DNGTYFQNIELLKTEVDRAQTRAELSGRTFSFGTDFFPAGTSSGEAEGQLVYVGHGWV
                     VKSKNVNAYEGLDVKDKIMVVSRVGNMPPGMTARDMKALPAGDWEDPDSYARRNGAKG
                     IVYVPANYERVMRSSSSMNTRPSYRVARFVDEAAGEGATKAPAAPAPVSIYPTATMLD
                     ALFAGEAATGAEVLRKTKEGAPPKGFALSPGKRLRLSVSMKADTATTRNVVAVLEGRD
                     KDLKKEYVAVGAHYDHVGVGRADARGDTIYNGADDDGSGTTAILAMAEAFAKGKRPRR
                     SILFVWHTGEEKGLWGSEYFTKYPTVPLEAIVAQLNIDMIGRSRAAGDTKPANAMLSG
                     PDEIYVIGSKMMSTELASWSEGVNGAYLNLKFNYHYDDPADTERLFYRSDHYNYAKMG
                     IPIIFYFDGIHEDYHKPSDTADKIDYQKMEKVTRTIFILASEIANNQRRPVVDKRLSS
                     DRLGR"
     gene            4928..5000
                     /locus_tag="AC159P_17395"
     tRNA            4928..5000
                     /locus_tag="AC159P_17395"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4961..4963,aa:Lys,seq:ctt)
     gene            complement(5180..5638)
                     /locus_tag="AC159P_17400"
     CDS             complement(5180..5638)
                     /locus_tag="AC159P_17400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697271.1"
                     /translation="MFCPKCGQEQTSESVRFCSRCGFKLNTVEEPLAKRLGKMTMFLV
                     LAVCAIMGWASMTAGPSYMEVRVITTLIAVFTFYLLFSRDLKRIFDRSFSRNIEHVKQ
                     LTPAVENPALPPAQSIPVSAFGPHRVNTAEMVQPPSITEQTTTLLDKGKR"
     gene            5790..6299
                     /locus_tag="AC159P_17405"
     CDS             5790..6299
                     /locus_tag="AC159P_17405"
                     /inference="COORDINATES: protein motif:HMM:TIGR01662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HIR3697272.1"
                     /translation="MLHTQPFKLYVFDADGTLRRSTVPGQPCPNLPGDWELIPGVRER
                     LAEIDWGPAGSSFGVATNQGGVGLGYLTLGMARRLLDEMVVQAFGFEPPPGSIEICPH
                     APHTGCACRKPNPLMLLRLLRRFRAKPGETLFVGDMERDEEAARRAGMHFIWAHDFFG
                     WPEQPSCDS"
     gene            6417..6731
                     /locus_tag="AC159P_17410"
     CDS             6417..6731
                     /locus_tag="AC159P_17410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697273.1"
                     /translation="MSKTAKIHDFTPAERRAARAEGVSVHEAEPYGWIARTADGDERG
                     YHLFCDPETRHLACTCADFVFRGNADSGYECKHVVATLRFIARRYLATQYDPFKQRAR
                     VA"
     gene            complement(6826..>7555)
                     /locus_tag="AC159P_17415"
     CDS             complement(6826..>7555)
                     /locus_tag="AC159P_17415"
                     /inference="COORDINATES: protein motif:HMM:NF014867.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="lactate/malate family dehydrogenase"
                     /protein_id="HIR3697274.1"
                     /translation="LAGADIAVIAAGVGSRPGESRLDLLGRNVVIIRECVRGLLSAGF
                     EGIVLMTTNPVDILAQVAQAESGLPAGRVISSGTVLDTARLRSMLGERLGVEARSIHA
                     YVVGEHGDSEVAAWSSARIAGVPLKDYCAAAGADVDFEELLRRVRGAAPEIIRHKGYT
                     STAIASCVARICEAILRDEHTVLPVSTLVTGQYGIDGVYLSLPCVVGREGVERVIEIP
                     LDEEELAGLRASASVLRQTLAQVG"
BASE COUNT         1316 a         2527 c         2417 g         1295 t
ORIGIN      
        1 cgggcgcgat ggtcttgccc gtcatgtcga tccgcttcgc gcccgaaggt atctgaagcc
       61 gcgcgccgac cgccttgatg cggttgtctt cgacgaggat ggtgcccttc tcgatgactt
      121 cgtcgccgcg catggtgacg aggcgcgcgt tcaagagggc gacgagtccg cgcgggcgcg
      181 ccgtctcgaa gtcgaagccg atgacggtcg cgcgcggggg cggcggcttc tcgtccttgc
      241 cgagcgcctt gttgatgttc tccagactct gctcgtagaa gttctcgccg agcgtccact
      301 gcaccgactt cgagtcgggc gaccacgcga gccagttgcc cgcctgcggc gaggcgggac
      361 ggacggggac gttcgtctcc gtcgagctga gcttgacggt cttgccgatc tgcgggaacg
      421 gcgcgacgta gagcttgtgc agctcgcgga aggcgatcca cttgtggtcg ggcgagggga
      481 tgatctcggt gacgtaggcg ccctcgatgt ggcgcttgaa gtcgtcgccg ccgagcttga
      541 cgctcgaaag atacgtgatg aactgcgcgg gcgcggtcgg cgtcggcgcg gggccgccgc
      601 ccgcctccat gaagaagatg cgctcgccct ccgggtcgaa gctcaagacc ggcatccgca
      661 ggttcggccc gcggctctgc aagtccatca cgtagctgtg cgcgcgcccg tcccagtagt
      721 gaatctcgaa actcgactcg ctcgccaggt tctcttcgtg gtagacgcta ccgcgcccct
      781 tgaggtaggc gatcttcgac ccgtcgcgcg agaagacggg gttggcgtag aggtctggcg
      841 ccgtcgtgat cctgacgggg ttcgtcccgt ccgcgttcgc cgtccacacg gcgcccttct
      901 cgtcgtcgcg ccaggtgacg tacgcgatgc gcctgccgtc ggcgctgaag cgcggcgcgt
      961 attcgaggta cttcgagttc aggagcgggc gcggcgcggc ctcgccctcc ttcacgtaaa
     1021 tcctcccgag cgcgctgtag atgatgcggt cgccattgcg ctcggcccag cgcaggagcc
     1081 gcgcgcgctc ccgctctgcc gcgaccttga cgggcgagcg caccgcctcg ctcacacgct
     1141 gcgcgacctg cgccttgaag gggatcggcg tcgcctgtct gctttcgacg ttgacgcgca
     1201 cgaacttgcc ctgcgccgtg atgacgaccg ccttcgagtc gggcgtccaa gcgtagccgg
     1261 ggtaggtgcc gaagatcgcc cacgtctcct gctggtcgcg cgtgagttgg tcgtagacgg
     1321 gccactcgcg ccccgtctcg atctcgcgca tgaagagcac cgtcttcaaa ccgacgcggc
     1381 ggatgaagga gaggagcttc ccgtcgggcg agatggccgg gcggatcgcg ccgcccgcgc
     1441 cgcggatgaa gaccgagcgg cgaccttttt gcaagtcgta gcgctcgatc cagtagatgc
     1501 tgtcgtagac gttcttgttg tagtcgaagg cgcccgaagc gacgaagtac atgaagcgcc
     1561 ccttcggctc gaccaccggc tcgccgatgt tcgccgtcca gttcggcttc tcggtgagct
     1621 gcacgccctt gccgccgccg ttcacgtggt acatccagat ttcgcccgcg cccgccgagc
     1681 gcgtgtccgt ccagtgcttg cgcgccagca ggtactgccc gtccggcgtc cacgccgggg
     1741 tgttcacgag acgcgtcgtc tccttcgtca cctgccgccg gttcttccct tccgcgtccg
     1801 cgacccagat gttgtcgccg ccgtcgcggt cagaggtgaa cgcgatccac ttgccgtccg
     1861 gcgaccagcg cggctgcacc tcccacgacg cgccgcccga cacgagccgc gcctcgccgc
     1921 ccgccacggg catcacgtag atgtcgccca gcaggtcgaa cacgagccgc tgcccgtccg
     1981 gcgacacgtc gcagctcatc cacgtccctt cgtccgtgtc gaactcgacc agcgtcgagg
     2041 ggccgtggtc gccctccacg tcccacggct cgaacttctt cgccgcttcc ggctcggcct
     2101 tcttcggcgc gtcctgcgcc cggctgtcac cacccgctcg gttccgcgcg gacggccacg
     2161 cctgcgcgtt caggagaagg gagagcatca acaggcagac ggggaatctt ctcaacacgt
     2221 ttcacctctc gcgcggaaag tttcgacgac ttgtgcaaag gaatggggca ggctctacag
     2281 agcgcgcaca ttctagccga catcggcggc cttgcgccag aagtgttctt gtctcggaat
     2341 ccgcgcataa ctcctggcct cccgcccacc tcttgaggag gacacatgaa caaatacgag
     2401 tacggcaaca ggagcggagg catcgtcctt tgtgcctatc tcgaagcggg tttcggcgtc
     2461 agcatcccgt tcggcgcggg tgccagctat gacttgatcg ttgactgcgg cgcgcgcctt
     2521 ctcaaagtgc aggtgaagac cgcgtggcta agcggcgggt gcgtggttta caagagccag
     2581 cgcagacaac ccggcgccga tctcactcga aggccgtaca gaaagggaga agttgactac
     2641 ttcgtcgcgt actacccggc taacgaaacg ctctacgcgg tgcccgccga aaaccacggt
     2701 aacgaaggaa gactcagaat cgagccggtg aagaacgggc aggcgaaact ggtgaagtgg
     2761 gcggcggact tcacttggga acggcatatc gagatgttga ggaatgaata cgcccgagag
     2821 gattcgaacc tctggcctcc gcctccggag ggcggcgctc tatccaactg agctacgggc
     2881 gcgcagtggt gattctcgca caagactagg ggttgtgcaa aacgatggac gtgtgatgcg
     2941 gcggttcgcg cgaccggcct ttcgcggctg gcgaacgtta gcctttccgt ctatcatcaa
     3001 aggcaaactg ctcacgcagg cgcaaactga acagtcggag gactcatgcg aaattcgaag
     3061 ttgtacgccc tggcgctggc cgtcgcgttg ctgacgctgc ccgtggcgcc gccgcgggcg
     3121 cagcaggcgc ggaaggcgac cgctgccgcg acgaaggcag cggtgccgtc cgccgccttc
     3181 gccgccgcgc gcaaggacgc cgaggagatc accgcggcga agatgaagga ggtgctgtac
     3241 tacatctctt cggacgcgat ggcggggcgc gacacgccgt cggaggggct ggacaagacg
     3301 gctgagtaca tcgccgaccg cctcgccaag ttgaagctca agccggcggg cgacaacggc
     3361 acctacttcc agaacatcga gctgctcaaa accgaggttg accgcgcgca gacgcgcgcc
     3421 gagctgtcgg gccgcacctt ctcgttcggg acggacttct tccccgccgg cacgtcgagc
     3481 ggcgaggccg agggccagct cgtctacgtc ggccacgggt gggtcgtcaa gtcgaagaac
     3541 gtcaacgcct acgaggggct ggacgtgaag gacaagatca tggtcgtctc gcgggtcggc
     3601 aacatgccgc cgggcatgac cgcgcgcgac atgaaggcgc tgcccgcggg cgactgggaa
     3661 gaccccgaca gctacgcccg ccgtaacggc gcgaagggga tcgtctacgt gccggccaac
     3721 tatgagcggg tcatgcgttc gagttcgagc atgaacacgc gcccgagcta ccgcgtcgcg
     3781 cgcttcgtgg acgaggccgc cggcgagggc gccacgaagg cgcccgccgc gcccgcgccc
     3841 gtctcgatct acccgacggc cacgatgctc gacgccctct tcgcgggcga ggcggcgacc
     3901 ggcgccgagg tgctgcggaa gacgaaggaa ggcgcgccgc cgaagggctt cgcgctctcg
     3961 cccggcaagc ggctgcggct ctccgtcagc atgaaggccg acacggcgac gacgaggaac
     4021 gtggtcgccg tgctcgaagg ccgcgacaag gacttgaaga aggagtacgt cgccgtcggc
     4081 gcgcactacg accacgtcgg cgtgggccgc gcggacgcca ggggcgacac catctacaac
     4141 ggcgcggacg acgacggctc gggcaccacg gccatcctcg cgatggccga agccttcgcc
     4201 aaaggcaagc gtccgcggcg ctcgatcctc ttcgtctggc acaccggcga ggagaagggc
     4261 ttgtggggtt ccgaatactt cacgaagtac ccgaccgtgc cgctcgaagc catcgtcgcg
     4321 cagctcaaca tcgacatgat cggtcgcagc cgcgccgccg gcgacacgaa gcccgccaac
     4381 gcgatgctct cggggccgga cgagatttac gtcatcggct cgaagatgat gagcacggag
     4441 ctggcgagct ggagcgaggg cgtcaacggc gcctacctca acctcaagtt caactaccac
     4501 tacgacgacc cggcggacac cgagcgcctc ttctaccgca gcgaccacta caactacgcc
     4561 aagatgggca tccccatcat cttctacttc gacggcatcc acgaggacta tcacaagccc
     4621 tcggacaccg ccgacaagat cgactaccag aagatggaga aggtcacgcg caccatcttc
     4681 atcctcgcct ccgagatcgc caacaaccag aggcgccccg tcgtggacaa acgactttcg
     4741 tccgaccgcc tgggtcgctg accgaggcag cggctgtaga cgaaaacccg taggcgggag
     4801 gtgaggccga ggcccgacag ccgcgccctc accctcccgc cttcgtcttc catcgaggga
     4861 cgatgcgggc cgccgcgggc cttcttgaca tcatcgcttc gccccgtata attggtttca
     4921 gtcttgcggg ccgatagctc agttggtaga gcagcggact cttaatccgc gggtcgcagg
     4981 ttcgatccct gctcggctca tcaatccgta gacgtacagg actgagctcg cttcaccgct
     5041 ccgccggaca ggtcgtttga ccggatggcc aagcagaaag ggctaagtcc ttatgttttc
     5101 gggtgagctt ttcgagcagg tcgtcggcga cggaatccat tcgagaaatg tcacgcctcg
     5161 caccgcggca ttgcctgttt caacgtttgc ctttatcgag cagcgtggtc gtttgctccg
     5221 taatactcgg cggctgaacc atctccgccg tgttcacgcg atgcgggccg aaagccgaca
     5281 cggggatact ttgtgcgggc ggcaaagcgg ggttttcaac ggcgggggta agttgcttca
     5341 catgttcgat attccgtgag aacgatctgt cgaatatgcg cttcaagtcg cgggaaaaga
     5401 gtaggtagaa ggtgaagacc gcgataagcg tggtgataac tcgtacctcc atgtagctag
     5461 ggccggctgt catcgaggcc caccccatta tggcgcacac cgccaatacg agaaacatgg
     5521 tcatcttgcc caaacgtttg gctaacggct cttcgacagt gttcaacttg aatccgcaac
     5581 gcgaacagaa gcgcacggat tcggaagttt gttcctgacc gcatttcggg caaaacatgg
     5641 aatcacctcc ctcgaaggat acgaaactat gttacagcta gtttgcgaat tatcgcatcg
     5701 gtcagcgccc agcgcccccc attgatccga accgcaacac ggtctacctt tattcgagat
     5761 tgattcgccg ggccgttgcc gcacgtggaa tgcttcacac gcagcccttc aagctctacg
     5821 tcttcgacgc cgacggcacg ctccgccgct cgaccgtgcc ggggcagccg tgcccgaacc
     5881 tgccgggcga ctgggagttg ataccgggcg tgcgcgagcg cctggcggag atcgactggg
     5941 ggccggcagg gtcgagtttc ggcgtcgcca cgaaccaggg cggcgtcggg ctgggatacc
     6001 tgacgctcgg catggcgcgc aggctcctcg acgagatggt cgtgcaggct ttcggcttcg
     6061 aacccccgcc cggctccatc gaaatctgcc cgcacgcgcc gcacacgggc tgcgcctgtc
     6121 gcaagccgaa cccgttgatg ctgctccgcc tgctgcggcg cttccgcgcg aagcccggcg
     6181 agacgctctt cgtcggcgac atggagcgcg acgaagaggc cgcccgccgc gccggcatgc
     6241 acttcatctg ggcgcacgat tttttcggct ggcccgaaca gccgtcctgc gattcctgat
     6301 caccccgaaa gcgtagagcc acgcctcgcc ccgccccgtt gatttatctc ggccccgctg
     6361 cgtttcatgc gtgcaacgcg gtttcactct cgcatatttt gtgttatatt gcccgcatgt
     6421 caaagacggc gaaaatccac gatttcaccc ccgccgagcg acgcgccgcg cgggcggaag
     6481 gcgtctccgt acacgaggcg gagccttacg gctggatcgc gcggacggcg gacggcgacg
     6541 agcgcggcta tcacctcttc tgcgacccgg agacgcggca cctcgcctgc acctgcgccg
     6601 acttcgtctt ccgcggcaac gccgactccg gctacgaatg caagcacgtg gtcgcgactt
     6661 tgaggttcat cgcccgccgc tacctcgcca cgcagtacga ccccttcaag cagcgcgccc
     6721 gcgtggcctg aaacggaagg gggaaaaggg aggatgagaa gcaccgtgct cgtcatcttc
     6781 cctcttcgcc cttcttcctc ttcccctctt aaccttttcc ccttcttaac cgacttgcgc
     6841 gagcgtctgc ctgaggactg aggccgaggc gcggaggccc gcgagttctt cttcgtcgag
     6901 tgggatttcg atgacgcgct cgacgccctc gcggcccacg acgcagggca ggctgaggta
     6961 aacgccgtcg atgccgtact ggccggtcac gagcgtcgag acgggcagca ccgtgtgctc
     7021 gtcgcgcagg atcgcctcgc agatgcgcgc gacgcacgag gcgatggccg tcgaggtgta
     7081 gcccttatgc cggatgatct cgggcgcggc gccgcgcacg cgccgcagca actcttcgaa
     7141 gtcaacgtcc gcgcccgccg ccgcgcagta gtctttgagc ggcacgcccg cgatgcgcgc
     7201 cgaactccac gcggccacct cggaatcgcc gtgctcgccg acgacgtagg cgtgaatcga
     7261 gcgcgcctcg acgccgagcc gctcgcccag catcgagcgc agccgcgccg tgtcgagcac
     7321 cgtgcccgag ctgatgacgc gcccggcggg caggcccgac tctgcttgcg ccacctgcgc
     7381 gaggatgtcc acggggttcg tcgtcatcag cacgatcccc tcgaaccccg cagagagcag
     7441 gccgcgaacg cactcgcgga tgataacgac gttgcggccc agcaggtcga gccgcgactc
     7501 gccgggtcgg ctgccgacgc ctgccgcgat gacggcgatg tccgccccgg cgagt
//
LOCUS       DBNGOH010000896         5519 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3617108, whole genome shotgun sequence.
ACCESSION   DBNGOH010000896 DBNGOH010000000
VERSION     DBNGOH010000896.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5519)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5519)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5519
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3617108"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..299)
                     /locus_tag="AC159P_17420"
     CDS             complement(<1..299)
                     /locus_tag="AC159P_17420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697275.1"
                     /translation="MDGTFEAIGLEVPSEKDFNYLAERAGDQGEPTQLPRRGAVLHGR
                     CWKLGEGLEVWTVLYESGTGEIFYADCRPGFRARYTQRITPWALTEYDEDGEAIV"
     gene            complement(367..603)
                     /locus_tag="AC159P_17425"
     CDS             complement(367..603)
                     /locus_tag="AC159P_17425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697276.1"
                     /translation="MYRKPRFLEELHAIREEMSREADYDVELFAEMVRTGARPAYGPA
                     RNIRGFRVRAPRADDMAEEVKEAKPSRRRRVGRS"
     gene            complement(804..1277)
                     /locus_tag="AC159P_17430"
     CDS             complement(804..1277)
                     /locus_tag="AC159P_17430"
                     /inference="COORDINATES: protein motif:HMM:NF013970.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HIR3697277.1"
                     /translation="MKQSIYLETSVVGAYLDNGEPFRRDLTIRWWEHEMSEYRAVVSP
                     LVTRELERVPEPHRTGYLKLVHKLEQVELTDEAAILADGYLSRGIFHRKYIGDALHVA
                     VASVHKIDYLVTWNFGHLANVRRQARIRLFNTAAGFFVPHIVTPEFLVSELTEKQ"
     gene            complement(1274..2893)
                     /locus_tag="AC159P_17435"
     CDS             complement(1274..2893)
                     /locus_tag="AC159P_17435"
                     /inference="COORDINATES: protein motif:HMM:NF025341.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl protease family protein"
                     /protein_id="HIR3697278.1"
                     /translation="MNRLFRPAVGVVAAIGFCAALVCAAPGAAVAGGDAKARARGERA
                     LRSGEFETAEKIFREVLVKDPKDVAARLGLSHALLKQRKARDAYDQAARVVAMEPVSA
                     RAHALLGAALLASGDFRLSVEEFRTALSFDGDEAMAIGGMAMVDFYENRLRASLEGLR
                     RASYLQPDEPDYVFNLGQVAARFERYTEAANAYEQFLRVAPRTDTDRRDRIRGLIDFL
                     RYLGTQKELVSVSGPSRVVVPFELVNNRPVVSVRINGSKEPLRFVVDSGAGMCVVAES
                     TANRLGLKPVARGGLARAIGGGGRFDIVYGFLQSLQMGEARLGNVPVYIRQFYNQHEQ
                     VDGYIGLSILSKYLAVIDYGAQSMTLQKRGEARPRYLNGEPDSFAPPTPHGIELPIRT
                     TSSGFWSGEVKIDSIERPFNFIIDTGATISVVSEALAVRESMDRYARTERIRVYGAAG
                     LTENIQTLLLPRLHIGTHARPNVYAAVISMEAINETAGFEQTGIVGGNVLRFYRVTFD
                     FDRAVVRLEPLTGASPTDSPKGAGQVVTPQS"
     gene            complement(3013..3675)
                     /locus_tag="AC159P_17440"
     CDS             complement(3013..3675)
                     /locus_tag="AC159P_17440"
                     /inference="COORDINATES: protein motif:HMM:NF046610.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxoguanine DNA glycosylase"
                     /protein_id="HIR3697279.1"
                     /translation="MRRDREPVTLEKIRAAHGERAAEIRARLAEFRDVWRAGTDERLW
                     EEMVYCIFTAGASARMGLGSVEAVRPLLGRGTHEELAGALASKHRYPNARAGYSVVTR
                     ESLTGDCRMRLRERLESFGDPAERRDWLARERGIKGLGYKEASHFLRNVGYSGYAILD
                     KHICRSLAELGVAASPEPPATRARYLALEDRMRRFAREVPIDFDELDLVLWSMKTGEV
                     LK"
     gene            4050..5444
                     /locus_tag="AC159P_17445"
     CDS             4050..5444
                     /locus_tag="AC159P_17445"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HIR3697280.1"
                     /translation="MSGSKIKFHFLALAVLLAPLAGARAQVSTRPAAPQQTQQQPPPV
                     APSLPTPTPTPLYIESHDAADDVLQQLAARPVVFRQGVVVERLDGETVTEQAAEQAFN
                     PASAVKLITALVALRTFGPKHRFATAMWITGTFDKATGTVTGDLVISGRDPSFHYEHA
                     VAVARELNQLGIRTVTGDLIVAPRFSMNFSPSSLRSGEAFYDTLDVTRRPAAATRAWN
                     DTRVALGDAKGLLETPSVAVMGAVYVNSVPPGARVVSTHYSPELTDVLKVLLCYSNNF
                     MADRLGDQLGGPEGLKRFLVTEVGLPAGEVRLASASGLGVNRVTPRHMMKIYRALLAE
                     LKENKLKAADIMPVAGVDPGTLDKRYLNSPGRGSVIGKTGTLGRTDGGVSALVGQMRA
                     KNGDVLLFVIFNQRGNVRAFRAAQDALVHELQFARGGPLPFTYTPQTLAMRLSASEFD
                     SAAATEEYEPESNQ"
BASE COUNT          889 a         1869 c         1821 g          940 t
ORIGIN      
        1 tggacgatgg cctcgccgtc ctcgtcgtac tcggtcaagg cccacggcgt gatgcgctgc
       61 gtgtagcgcg cgcgaaaacc cggtcggcag tcggcgtaga aaatctcgcc cgtccccgac
      121 tcgtagagca ccgtccagac ctcaagccct tcgccgagtt tccagcaccg cccgtgcagc
      181 acggcgccgc gccgcggcaa ctgcgtcggc tcgccctgat cgcccgcgcg ctccgcgaga
      241 tagttgaagt ccttctcgct cggcacttcc aacccgatgg cctcgaacgt cccgtccata
      301 aactcgcagt ggtcagtgtc agagtggccg gcggtcagcc gaaacgcgac ccgccggcgt
      361 cggtcgtcaa ctcctgccga cgcgccttct gcgcgaaggc ttcgcttcct tcacttcttc
      421 ggccatgtcg tcggcgcgcg gcgcacggac gcggaagccg cggatgttgc gcgccgggcc
      481 gtacgcgggg cgcgcgccgg tgcgcaccat ctcggcgaac agctccacgt cgtagtcggc
      541 ctcgcgcgac atctcctcgc ggatcgcgtg cagctcttcg aggaacctcg gctttcgata
      601 catgatggta aatcgtaaac ggtaaatggt aaatagtgaa tggtgaatcg gaagctgtta
      661 gctgttggcc gttggcacgc ggttcaaccg atgatgattg atgcgagcgc cgctgtcatc
      721 aatcgtcgtc gatccgtaag ccaacggcta agagctgatc gctaacaacc ccttatctcc
      781 attcactatt taccatttac tattcactgt ttctctgtca actccgaaac gaggaactcg
      841 ggcgtcacga tgtgggggac gaagaagccg gcggcggtgt tgaagaggcg gatgcgcgcc
      901 tgccgccgga cgttcgccag gtggccgaag ttccacgtca ctagataatc tattttgtgg
      961 acggaagcaa cggcgacgtg gagcgcgtcg ccgatgtact tgcggtggaa gatgccgcgc
     1021 gagagatacc cgtcggcgag gatggcggcc tcgtccgtca gctcgacctg ttcgagtttg
     1081 tggacgagct tgaggtagcc ggtgcggtgc ggctcgggca cgcgctccag ctcgcgcgtg
     1141 acgagcggcg agaccaccgc gcggtactcc gacatctcgt gctcccacca gcggatggtg
     1201 aggtcgcggc ggaacggctc gccgttgtcg agataggcgc cgacgaccga ggtctccaga
     1261 tagatgctct gcttcatgac tgcggggtga cgacctgccc cgcgcccttc gggctgtcgg
     1321 tgggcgaggc gccggtgagc ggctccagcc ggacgacggc gcggtcgaag tcgaacgtca
     1381 cgcggtagaa gcgcaggacg ttgccgccga cgatgccggt ctgctcgaac ccggcggtct
     1441 cgttgatcgc ctccatgctg atgacggcgg cgtagacgtt cgggcgcgcg tgcgtgccga
     1501 tgtgcaggcg cggcagcagc agcgtctgga tgttttcggt cagcccggcg gcgccgtaga
     1561 cgcggatgcg ctcggtgcgc gcgtagcgat ccatgctctc gcgcacggcc agcgcctcgg
     1621 agacgaccga gatggtggcg cccgtgtcga tgatgaagtt gaacgggcgc tcgatgctgt
     1681 cgatcttcac ctcgccgctc cagaagccgc tcgaagtcgt gcggatcggc agctcgatgc
     1741 cgtggggcgt gggcggcgcg aagctgtccg gctcgccgtt gaggtagcgg ggccgcgcct
     1801 cgccgcgctt ttgcagcgtc atcgactgtg cgccgtagtc gatcaccgcc agatacttcg
     1861 acaggatcga gaggccgatg tagccgtcca cctgctcgtg ctggttgtag aactggcgga
     1921 tgtagacggg cacgttgccg agccgcgcct cgcccatctg caacgactgg aggaagccgt
     1981 agacgatgtc gaagcgaccg ccgccgccga tggcgcgggc gaggcccccg cgcgcgaccg
     2041 gcttgaggcc gaggcggttc gccgtgctct cggcgacgac gcacatcccc gcgccgctat
     2101 cgacgacgaa gcgcagcggc tccttcgagc cgttgatccg cacgctgacg accgggcggt
     2161 tgttgaccag ctcgaacggc accacgacgc gcgaagggcc gctgacgctg acgagttctt
     2221 tctgcgtgcc gaggtagcgg aggaagtcga tgaggccgcg gatgcggtcg cggcggtcgg
     2281 tgtcggtgcg cggggcaacg cgcaggaact gctcgtaggc gttggccgcc tcggtgtacc
     2341 gctcgaaccg cgccgctacc tgcccgaggt tgaagacgta gtccggctcg tccggttgca
     2401 ggtacgaggc gcggcgcaag ccttcgaggc tggcgcgcag gcggttctcg taaaagtcca
     2461 ccatcgccat cccgccgatg gccatcgcct cgtcgccgtc gaagctcagc gccgtgcgga
     2521 actcctcgac cgagaggcgg aagtcgcccg aggcgaggag cgccgccccg agcagcgcgt
     2581 gcgcgcgcgc cgacaccggc tccatggcga cgacgcgcgc cgcctgatcg tacgcgtcgc
     2641 gcgccttccg ctgcttcaac agcgcgtggc tgaggccgag acgcgccgcc acgtccttcg
     2701 ggtctttcac cagcacctcg cggaaaatct tctccgccgt ctcgaactcg ccgctgcgca
     2761 acgcgcgctc gccgcgcgcc cgcgccttcg cgtcgccgcc ggccacagcc gcgcccggcg
     2821 ccgcgcacac cagcgccgcg caaaagccga tggcggccac cacgccgacc gccggacgga
     2881 agagtctgtt catggcatca actcctagtt cgattgcgga atgcggattt cggattgcgg
     2941 attgaagcag caaagatgat tgatgacagc gcggctctcg tcaatggtta atccgaaatc
     3001 cgcattccgc attcacttga gcacctcgcc cgtcttcatc gaccagagga cgaggtcgag
     3061 ttcgtcgaag tctatcggga cttcgcgggc gaagcggcgc atccgatctt cgagggccag
     3121 gtagcgggcg cgcgtcgcag gcggctcggg cgaagcggcg acgccgagtt cggcgaggct
     3181 gcggcagatg tgcttgtcga ggatcgcgta gccgctgtag ccgacgttgc gcaggaagtg
     3241 actcgcctct ttgtagccga ggcccttgat gccgcgctcg cgcgcgagcc agtcgcgccg
     3301 ctccgccggg tcgccgaaac tttcgagccg ctcgcgcagg cgcatccggc agtcgcccgt
     3361 cagggactcg cgcgtcacca cgctgtagcc cgcgcgcgcg ttcgggtagc ggtgcttcga
     3421 cgcgagcgcc ccggccagct cctcgtgcgt gccgcgcccg agcagcgggc gcacggcctc
     3481 gaccgaaccg aggcccatcc gcgccgacgc gcccgccgtg aagatgcagt agaccatctc
     3541 ctcccacagc cgctcgtcgg tgcccgcgcg ccacacgtcg cggaactccg cgaggcgtgc
     3601 gcgaatctcc gcggcgcgct cgccgtgcgc ggcgcgaatc ttttcgagtg tgacgggttc
     3661 tctgtctcgc cgcacgatgg attatcaaac agtagcgaga tggactgacc actgaatttt
     3721 attggcgcgt tccgagcaat gtcaaaagaa agtgagcagt aagctgtaag cagtaagcgg
     3781 taaagagttt cggcattcga cggccacggg catccaataa gaactgctca ctgctcacag
     3841 ctcactgctc actttccgtc ggaatgtggc ttgctttgtg cgtttcagcg gagcatggcg
     3901 cgcatgcatc tcgtacgatg cgcttgttca tctaaagtgt tggcccgcgg tgggtgcgcc
     3961 cgccgcatta ttttacaggc gcgccgggcg ccgcccgcgc gtgtgctatc atgtcgcgcc
     4021 ctcccgtgtg cgggagtcga aaataagcaa tgagtggttc gaagattaaa tttcacttcc
     4081 tcgcactggc cgtcctgctc gcgccgctcg cgggcgcgcg cgcgcaagtc tcgacgcgcc
     4141 ccgccgcgcc gcagcagacg cagcagcagc cgccgccggt cgcgccgtct ctgccgacgc
     4201 cgacgccgac gccgctctac atcgagtcgc acgacgcggc ggacgacgtg cttcagcagc
     4261 tcgccgcgcg ccccgtcgtc ttccggcagg gcgtcgtcgt cgagcggttg gacggcgaga
     4321 ccgtcacgga gcaggccgcg gagcaggcgt tcaaccccgc ctccgccgtg aagctcatca
     4381 ccgcgctggt cgcgctccgc accttcggcc cgaagcaccg tttcgccacg gccatgtgga
     4441 tcacaggcac cttcgacaag gcgaccggca ccgtcaccgg cgacctcgtc atctcgggac
     4501 gcgacccctc tttccactac gagcacgccg tcgccgtcgc gcgcgagctg aaccagctcg
     4561 gcatccgcac cgtcaccggc gacctcatcg tcgccccgcg cttctcgatg aacttcagcc
     4621 cgtcgagcct ccgctcgggc gaagccttct acgacacgct cgacgtgacg cgccggcccg
     4681 cggcggccac gcgcgcgtgg aacgacacgc gcgtcgcgct cggcgacgcg aagggcctgc
     4741 ttgagacgcc gagcgtggcg gtgatgggcg cggtctacgt caactccgtg ccgccgggcg
     4801 cgcgcgtcgt ctcgacgcac tactcgcccg agctgaccga cgtgctcaag gtgctgctct
     4861 gctactcgaa caacttcatg gccgaccgac tcggcgacca gctcggcggc cccgaagggt
     4921 tgaagcgttt tctcgtcacg gaggtgggct tgcccgcggg cgaggtgcgc ctggcgtcgg
     4981 cgagcggcct cggcgtcaac cgcgtcacgc cccgccacat gatgaagatt taccgcgcgc
     5041 tgctggccga gctgaaggag aacaagctga aggcggcgga catcatgccg gtcgcaggcg
     5101 tcgatccggg cacgctcgac aagcgctact tgaacagccc cggtcgcggc agcgtcatcg
     5161 gcaagaccgg cacgctcggg cgcacggacg gcggcgtgag cgcgctggtc gggcagatgc
     5221 gcgccaagaa cggcgacgtg ctcctcttcg tcatcttcaa ccagcgcggc aacgtccgcg
     5281 ccttccgcgc cgcgcaggac gcgctcgtcc acgagctgca attcgcgcgc ggcggcccgc
     5341 tccccttcac ctacacgccg cagacgctcg ccatgcgcct ctcggcctcc gagttcgaca
     5401 gcgccgccgc caccgaggag tacgagccgg agtcgaatca atgatgaata gaatgggtgt
     5461 tgggtgttag gtgttaggtg ttgggttctg gttttttgtc ttaacccaac acctaacac
//
LOCUS       DBNGOH010000897         3357 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1419152, whole genome shotgun sequence.
ACCESSION   DBNGOH010000897 DBNGOH010000000
VERSION     DBNGOH010000897.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3357)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3357)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3357
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1419152"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1308)
                     /locus_tag="AC159P_17450"
     CDS             complement(<1..1308)
                     /locus_tag="AC159P_17450"
                     /inference="COORDINATES: protein motif:HMM:NF047398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HIR3697281.1"
                     /translation="MGQIVTFYSYKGGVGRSMALANTAALLARWGYRTLIVDWDLEAP
                     GLENFYREYSSLQSVPDEKGVVDIISAAYPMMEPEGGDGHAGEAPRQQEEVEAPAEVE
                     LPPWRDYVVSIKIGFRDKAEGTLHLLTAGQRSSPEYFRKVRRFDVQDFYREQRGGYFI
                     ERLRREWKTEYDVVLIDSRTGVTDIGGICTIQLPDILVLIFTSTEHGLVGAADVARKA
                     TLARQSLPFDRRKLLTLPILSRFDDSVEFEISQRWLDRVEEVVKDLYADWLAADVRRR
                     DFVELTKIPYKAFFSFGEKLPVVEEGTVNRAGMAFAYETLAALLANRLEGAEQLRENR
                     DGFVRRAASAERSPAPAGPEVDRALVEQRVEGHEQWLLSNGAAGKAASFAGLYLRASD
                     LSSARLAGADFGKADLNAVNFASSVLEGADFSGASLQGANLRGV"
     gene            complement(1319..1984)
                     /locus_tag="AC159P_17455"
     CDS             complement(1319..1984)
                     /locus_tag="AC159P_17455"
                     /inference="COORDINATES: protein motif:HMM:NF025056.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HIR3697282.1"
                     /translation="MSAALNITARAEETYKYDVFISYPHEPDHHTWVHKYFLPKFRLY
                     LRQELFREPQIYVDDDRIAPGATWPLNLKKALARTRVLIPVWSIDYFISNWCKAECSV
                     MLYREKALNMRTGENDDGLILPVRLFDGKGYPEFAQKIQSLDCNDFNNIAGNFEQTPK
                     FFELVNVIKNWTPKVADRIRHSPEWSADWTTDAWLDEPIKQCGVSPDFRFPDRPFELE
                     GIG"
     gene            complement(2247..3008)
                     /locus_tag="AC159P_17460"
     CDS             complement(2247..3008)
                     /locus_tag="AC159P_17460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697283.1"
                     /translation="MKATLASLVLLTCAASAAQAQGVNADYLPFPQSRLRRFPLKRHA
                     FPPGAPMDGKPFTVSGVTMRDAQFEVSGATHGIEQVGRRLVFNGREKGGEEWRLETAS
                     WTYYDAVYEGDLDRNGVRDLVVSIGTGANGLGEPTHLIFLTFDRRGHPSLFEATGYFD
                     PRAGDIYYLADLDGDRRAELLFMTYDDGYWITNVYQPHESRWSRVNGRLAGLRFPLYT
                     RFTRRPNHTPARPAPGRSPHQPDLPEENGAARRRP"
     gene            complement(3074..>3357)
                     /locus_tag="AC159P_17465"
     CDS             complement(3074..>3357)
                     /locus_tag="AC159P_17465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697284.1"
                     /translation="IQDGVFVGDLGAGRGPGVAVWNLLWEDEAHYGAHRYEVRLAPFD
                     ARTASFGRGRQLRTKGKHANGRDALAELGLPRYANLLDEMPSIAEYRRH"
BASE COUNT          498 a         1105 c         1190 g          564 t
ORIGIN      
        1 cacgccgcgc aggttggcgc cctgaagcga agcgccggaa aagtcggcgc cctccagcac
       61 ggagctggcg aagttgaccg cgttgaggtc ggccttgccg aaatctgccc cggcaagccg
      121 cgccgaagag aggtcgctgg cgcgcaggta cagcccggcg aagctcgccg ccttgcccgc
      181 cgccccgttc gacaggagcc actgctcgtg cccctcgacc ctctgctcaa cgagcgcgcg
      241 gtctacctcc ggccccgcgg gcgcgggcga cctttcggcg ctggcggcgc ggcggacgaa
      301 gccgtcgcgg ttctcgcgca actgctcggc cccttcgagc cggttggcga gcagcgccgc
      361 cagcgtctcg taggcgaagg ccatgcccgc acggttgacc gtcccttcct cgacgaccgg
      421 cagcttctcg ccgaagctga agaaagcctt gtaggggatc ttcgtcagct cgacgaagtc
      481 gcggcggcgc acgtcagccg cgagccagtc ggcgtacagg tctttcacca cctcctcgac
      541 gcggtcgagc cagcgctggg aaatctcgaa ctcgaccgag tcgtcgaagc ggctgaggat
      601 cggcagcgtc aacagcttgc ggcggtcgaa ggggaggctc tggcgggcca gcgtcgcctt
      661 gcgcgccacg tcagccgcgc cgaccagccc gtgctcggtg gacgtgaaga tgaggacgag
      721 gatgtccggc agttggatgg tgcagatgcc gccgatgtcg gtgacgccgg tgcggctgtc
      781 gatgagcacc acgtcgtact ccgtcttcca ctcgcgccgc agccgctcga tgaagtagcc
      841 gccgcgctgc tcgcggtaga agtcctgcac gtcgaagcgg cgcacctttc ggaagtactc
      901 ggggctgctg cgctggcccg ccgtcagcag gtgcagcgtc ccctcggcct tgtccctgaa
      961 cccgatcttg atgctgacga cgtagtcgcg ccacggcggc aactcgacct cggcgggcgc
     1021 ctcgacttcc tcctgctgcc ggggcgcctc cccggcgtgg ccgtcgccgc cctccggctc
     1081 catcatgggg taggccgccg agatgatatc gacgacgccc ttctcgtcgg ggacgctctg
     1141 cagactcgaa tactcgcggt agaagttctc cagccccggc gcctccaggt cccaatcgac
     1201 gatgagcgtg cgatagcccc agcgcgccag cagcgccgcc gtgttggcga gcgccatcga
     1261 gcggcccacg ccgcccttgt acgagtagaa ggtgacgatc tgccccacgc gcgcacgcct
     1321 acccgatgcc ttccagctcg aaggggcggt cggggaatcg gaagtccggc gacacgccac
     1381 actgcttgat gggttcgtcg agccaggcgt cggtcgtcca gtccgccgac cactcgggcg
     1441 agtggcggat gcggtcggcc accttcggcg tccagttctt gatgacgttg accagctcga
     1501 agaacttcgg ggtctgctcg aagttcccgg cgatgttgtt gaagtcgttg cagtcgaggg
     1561 actgaatctt ctgcgcgaac tcggggtagc ccttcccgtc gaagagtcgc acggggagga
     1621 tgaggccgtc gtcgttctcg cccgtgcgca tgttgagcgc cttctcgcgg tacaacatga
     1681 cggagcactc cgccttgcac cagttcgaga tgaagtagtc gatcgaccag accgggatca
     1741 ggacgcgcgt gcgggcgagc gccttcttca aattgagcgg ccacgtcgcg cccggcgcga
     1801 tgcggtcgtc gtccacgtaa atctgcggct cgcggaacaa ctcctgccgc aggtagagcc
     1861 ggaacttggg caagaagtat ttatgcaccc acgtgtggtg gtcaggctcg tgcgggtagc
     1921 tgatgaagac atcgtacttg tacgtctctt cggccctggc cgtgatgttg agggctgcgc
     1981 tcatcttcgg gacttgcctc cgctgaggtt gacggtgcga cgagaccggt cacgtcgttc
     2041 gagagtcgcc gccggagtgc ggcggcatcg ggccgccttg cttccagcat ccctcgcgtg
     2101 agcaatcggc agccgacagg taaggcatgc tcttgtacag aaggtgaggg ccgcgcgtca
     2161 agcgaaaacc tttcggcgcg cgggcttcgc cgggcgggac gcgagcgggc ggcggggcgc
     2221 gtgtccgccc gccgccgcgc actccttcag ggccgtcggc gggccgcccc gttctcttcc
     2281 gggaggtcgg gctggtgcgg cgagcggccc ggcgcggggc gcgcgggagt gtggttgggg
     2341 cggcgcgtga agcgcgtgta gagcgggaat cggagtccgg cgagccttcc gttgacgcgg
     2401 ctccagcgcg actcgtgcgg ctggtagacg ttggtgatcc agtaaccgtc gtcgtaggtc
     2461 atgaagagga gttcggcgcg gcggtcgccg tcgaggtcgg cgaggtagta gatgtcgccc
     2521 gcgcgcgggt cgaagtagcc ggtggcctcg aagagcgagg ggtggccgcg gcggtcgaag
     2581 gtgaggaaga tgaggtgcgt cggttcgccc aacccgttgg cgccggtgcc gatggagacg
     2641 acgaggtcgc gcaccccgtt gcggtcgagg tcgccttcgt agacggcgtc gtagtaagtc
     2701 cagctagcgg tttcgagccg ccactcctcg ccgcccttct cgcgcccgtt gaagacgagg
     2761 cggcgaccga cctgctcgat gccgtgcgtc gcgccgctga cttcgaactg cgcgtcgcgc
     2821 atcgtgacgc ccgagacggt gaacggcttg ccgtccattg gcgcgcccgg cgggaacgcg
     2881 tgccgcttca gagggaagcg gcggagcctg ctctgcggga acggcaggta gtccgcgttg
     2941 acgccctgcg cctgcgccgc cgaagccgcg cacgtcaaca ggacgagcga ggccagcgtc
     3001 gccttcatca acaagcagtg tcgtttcatc gtcagtcgga acgcgcgggc gcgcggctct
     3061 tgcgcggggg gcgtcagtgg cgacggtact ccgcgatgga gggcatctcg tcgagcaggt
     3121 tcgcgtagcg cggcaagccc agctccgcga gcgcgtcccg tccgttcgcg tgcttgccct
     3181 tcgtgcgtag ctgccggcct cttccgaaag acgccgtcct cgcgtcgaag ggggcgaggc
     3241 gcacttcgta gcggtgcgcg ccgtagtgcg cctcgtcttc ccagaggagg ttccagacgg
     3301 cgacgccggg gccgcggccc gcgccgaggt cgccgacgaa gacgccgtcc tgtatgt
//
LOCUS       DBNGOH010000898        13797 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3252468, whole genome shotgun sequence.
ACCESSION   DBNGOH010000898 DBNGOH010000000
VERSION     DBNGOH010000898.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 13797)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13797)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13797
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3252468"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1337)
                     /locus_tag="AC159P_17470"
     CDS             complement(<1..1337)
                     /locus_tag="AC159P_17470"
                     /inference="COORDINATES: protein motif:HMM:NF012906.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HIR3697285.1"
                     /translation="MTTIHDSIRRRGARAVAYLFCLCFVFALCVPDASAQPARERASF
                     NADWRFQRRDPAGAEGALVYERIKGWINATGGEFVAGGSGRTRPEGNVGEDVAYTRRG
                     FDDGAWRRLNLPHDWGIEGPFDQALSGETGKLPWAGVGWYRKRFHVAAGERGRQLYLD
                     IDGAMAYSAVWLNGQLVGGWPYGYASFRLDLTPYVEYGAENVLAIRLDNPPESSRWYP
                     GAGLYRNVWLVKTAPVHVGHWGTYATTPEVTSEAATVDLKVTVDNRSNAAANVTLRTE
                     LYELGADGRRRAKPAATFAPAGLNVAAGQRATVGTSARVRNPKLWGVRQPNLYAAVTS
                     VEHGGKVIDRYETVLGIRTIKFDAARGFLLNGEPVRMNGVCQHHDLGALGSAINTRAL
                     ERQFEILKEMGVNAVRTSHNPPAPELLDLADRMGLLIMDEAFDAWRRSKKKND"
     gene            complement(1342..2373)
                     /locus_tag="AC159P_17475"
     CDS             complement(1342..2373)
                     /locus_tag="AC159P_17475"
                     /inference="COORDINATES: protein motif:HMM:NF016497.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HIR3697286.1"
                     /translation="MNATKIIFGLCCLMLGAAPAAQTTPASSTQGKGPVRLADVRMRD
                     VCILADEATKTYYAVSSTRVPVADARVPAVRAYKSKDLVTWEGPHIIFQTPRGLWGDA
                     DIKGIWAPELIAYKDRYYLFLTFDTDSLFPEQWRNWLPRVKRGSQVLVADSPLGPFKP
                     FRNRSTLPPDMMTLDATLWVEDGAPYMVYAHEWVQITDGTIEMIRLTDDLSETVGEPT
                     KLFAGSDAPWGRKSPQWGCWVTDAPYLYRSRTGKLLMIWSSFGEGGYTVGVARSTSGR
                     LRGPWVQQAAPLFAEDGGHGMIFKRFDGQLMLVLHQPNKERERARLFELEDAGDTLRI
                     KSPFPLART"
     gene            complement(2619..3602)
                     /locus_tag="AC159P_17480"
     CDS             complement(2619..3602)
                     /locus_tag="AC159P_17480"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR3697287.1"
                     /translation="MNYRELGRTGYSVSEIGFGAWAIGGTWGEVEDAESLAALHRAVD
                     LGVNFFDTADVYGDGRSERLLARLRRERDEEIIIATKAGRRLDPHVAAGYTAENLAGF
                     IERSLQNLETDALDLVQLHCPPTEVYYFPELFGALDRLVEQGKIRHYGVSVEKVEEAL
                     KAIEYPNVQTVQIIFNIFRQRPADLFFPEAKRRRVGILARLPLSSGMLTGKLTRETRF
                     AAEDHRAFNREGAAFDRGETFSGVDYETGLQAVEELRALVPEGATMAQFALRWILMFD
                     AVTCAIPGAKRPSQAEDNVRAADLAPLSEETMRAVRELYERRIRPLVHHYW"
     gene            complement(3643..4566)
                     /locus_tag="AC159P_17485"
     CDS             complement(3643..4566)
                     /locus_tag="AC159P_17485"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013568038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR3697288.1"
                     /translation="MSGKLVRAGVWASLLIVGFAVCATGPSAQTVGTTDQQTIVLWPE
                     GAPGARGREPQDTPTLTPYWPPKEKRTGAAVIVCPGGGYSRLAEHEGRPVAEWFNTLG
                     VTAFVLKYRLGPRYNHPAPLLDAARAIRTVRARAAEWGLDPARVGILGFSAGGHVAST
                     AGTHFDAGKSDAPDKIERVSSRPDLLMLIYPVITMRELTHAGSKKNLLGDAPKEELVA
                     LLSNEEQVTKETPPTFIVHTVNDAGVPVENSMMFASALRRAGVAFEMHLYERGRHGLG
                     LGTREGSADPVFATWPARCADWLRLYNFAAR"
     gene            complement(4759..5373)
                     /locus_tag="AC159P_17490"
     CDS             complement(4759..5373)
                     /locus_tag="AC159P_17490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697289.1"
                     /translation="MTRRFLVGCSLACALLFAVAASAAAPTNFAGTWVLDKSKSKDLP
                     QQWADNLESYTLTVSQDEKQLTVDNKINWKEGGGPGGGQASGAGGQGGPGGGGGGMGQ
                     GRGRGGMGRGMPTSLTYKLDGSETSMDWGDRGTSTQKAEWKDDGKALELTNVRKFTNR
                     DGEQRSSTSKERWELAADGKTLTINRTSETPRGEMKSTLAFNKQ"
     gene            complement(5574..8960)
                     /locus_tag="AC159P_17495"
     CDS             complement(5574..8960)
                     /locus_tag="AC159P_17495"
                     /inference="COORDINATES: protein motif:HMM:NF014851.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HIR3697290.1"
                     /translation="MSRNVHAAAARLLLLALAACWTATAAAQPAGRVPLAGEWGFALD
                     RADQGVRERWFSRGLAERIKLPGVLQSQGYGDEIGTETPWVLSLYDRQWFMREDFKAY
                     TAPGRVKVPFLSQPPRHYVGAAWYQRDIDIPAEWRGRRVVLFMERPRWESTVWVGERQ
                     VGANNSLSAPHAYDLGALAPGRHTLTVRVDSRMLLPYRPDAHSVSDSGGSSWNGIVGR
                     IDLSSTTPVFIEDAQVFPDVRKKSALIKVAIGNASGRAGRGTLSAAGASVPVTWDAQG
                     GRAELEVQLGPNAGTWDEFNPVVHRLDLRLEGEGADDRRELAFGLTEFRARGTDFLVN
                     GRKTHLRGTHHGGDFPLTGYPPTDVESWRRLIRTCKEWGLNHLRFHSWAPPEAAYVAA
                     DELGFYIQSEPGMWNEISPGTPMEKMLYEETERMIKAYGNHPSFVLLSASNEPKARWK
                     EALPRWAEHFRQRDPRRLYTTGTGHTEREIPNPTEGADFLVMQRIGPKWLRGQTAWFG
                     RDYRASLEGIDVPVASHELGQWVAYPDYDIIKKFTGYMRPGNYEIFRDSMKAKGLLDK
                     NKDFAWASGKYQLECYKEEIEANLRTPGMAGFQLLDLRDYLGQGTALVGLLDAFFESK
                     GYATPEEFRRFCNTTVPLARLTKRVFTSDETLDVEVEVAHYGPAPLEAASSSWRIVDA
                     GGRVVARGAWPARTLAHGNTRLGKVTADLSRLKAPAAYKLVVGLPGTVFENDWDFWLY
                     PSQANTAAPRDVLVTKSWDEAEARLSQGGKVLYVPAPAALDWTSPPLDRVPVFWNALM
                     GPLWGRMLGTWNDTRHPALAGFPTEAHFDWQWAEIIRNVRAVNMDRLPRELRPVVYAI
                     DDWNRNYRLGLVFEARVGRGRLLVSAFDLESALDERPVARTLRRSLLDYMASARFRPA
                     VALAPSDIRALMFDTRIMRRLGAVAQANAQASNDAANAIDGDPNTYWLVGDARNRDLK
                     HPHELTVTFPAPVAISGLVLMPRQNHREHEGDIRDYAVHASDDGATWRELLRGRLGST
                     FAPQQLRFPATVNARHLRLTALSGFGPDTTAALAELAVVYAGPRLAEDTDVTGVEYRR
                     GRTATTDIDAGTDAADRPAPPSARTTRRPAPRRRRTRARP"
     gene            9139..10077
                     /locus_tag="AC159P_17500"
     CDS             9139..10077
                     /locus_tag="AC159P_17500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697291.1"
                     /translation="MRLKRSASFVVLPSILLCLLPAGLLAAADKGAAHHVPAARERWG
                     DSPHHAGVISYHGGASAPTDARRAAAGRRDRKGGIREVVPDKYKGRYQEWKKEFLSTE
                     TGQQQWALFEKSEQLTLTIQVSRDNPKGAGTGQYKWDDEGKLVEATIVLGSSLHEGYP
                     NPIYYPVMNSLMQQEPTYAGSGHTLAATKIAHEFGHLTRTVEMDHALYELQNRLMPVY
                     NKILLSNGRNTSDPQLVELAQQMGGTPVEIWEDREYWGEANAMLFLRDRFAEEDLGCS
                     LFGKIKQSVNLYAKNYTERFLKVAQSSPSLNRCGWR"
     gene            complement(10224..12383)
                     /locus_tag="AC159P_17505"
     CDS             complement(10224..12383)
                     /locus_tag="AC159P_17505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014174529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65"
                     /protein_id="HIR3697292.1"
                     /translation="MVSRREFLGTLVASGGLYGSALSAPAAAQRGAGVIDRAALANRH
                     SPTLRKLDPLSPLSVGNGEFAFTGDVTGLQTFPGEYENAMPLCTMSQWGWHTSPRPQS
                     LEGAALRLVQYDTHGRQVGYQTSSENQAELFDWLRENPHRLHLGQLGLRLTRSDGTEA
                     RPGDITDIEQRLDLWSGTITSRFRFDGRPVTVRTSVHPRLDLLAVAVESSLIEEGRLA
                     VRLAFPYGSPKMHAADWGQPARHSSVLAARSVSRAEIRRALYEDRYSVALAWHTPATL
                     AAEKEHHFLLTPAARARRLEFVAHFAPRPAAQMLPTARATFAAAAAHWQRFWTTGGAI
                     ELAGSRDPRAHELERRVVLSQYLTAIQCAGSQPPQETGLTTNSWYGKFHLEMHWWHAA
                     HFPLWGRLPLLEKSLGWYDKVLPGARALARSQGYAGARWPKMVGPEGRDSPSPIGPLL
                     IWQQPHPIFYAELCYLAGGGRRALERYRRVVFETAEFMASFAFFDEQRGRYVLGPPVI
                     PAQENHPPRETWNPTFELAYWIYGLKTAQSWRERLGMKRDAKWDRVLSRLAPLPAREG
                     VYLAHENCPQTYTERNHDHPSMLGALGVLDGDGVDRETMRRTLRKVMKEWRWDETWGW
                     DYPMTAMTAARLGETETAIDALLLKTAKNNYSANGHNYQRPNLPCYLPGNGGLLYAVA
                     LMAAGWRGAPRTHAPGFPARSFQVRWEGLNAALLRQL"
     gene            complement(12572..>13797)
                     /locus_tag="AC159P_17510"
     CDS             complement(12572..>13797)
                     /locus_tag="AC159P_17510"
                     /inference="COORDINATES: protein motif:HMM:NF051698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HIR3697293.1"
                     /translation="YTRTTQISTITNANGAPIPVNIANFANIVVQEPVGNAQGRQTGL
                     GGAISFFNQNPEVSKQARWQIGLQRELPGGWLAEAVYVGNYGYNIEITRNINALPNQY
                     LNTDNSRTAAMNANNTFLTGTIANPFVGLFPAGSGLQAGTIARSQLLRPFPAFGDITT
                     TVNDGKSWYHSGQFRLEKRFTRNYTLQASYTWSKWLQETEYLNAGDAAPTKMISDQDV
                     PHRLSLSAMYAFPFGKNQRWLTDAGWLTNAIVGGWQIQGVYAYQSGFPIPFGSFNTGN
                     GNTAGDLFYNGGEIAIDNPTTERWFNTAAFTSLANATSTNAAPVNHLRTLPYRFSSVR
                     RDSINNVDFSLLKDIHIREGMRVQLRMELINAFNEAYFPNPVVNQTASNFGQISASNQ
                     ENYARRAQLGVKFLF"
BASE COUNT         2097 a         4463 c         4873 g         2364 t
ORIGIN      
        1 tagtcgttct tcttcttcga gcgccgccac gcgtcgaacg cctcgtccat gatgagcagg
       61 cccatgcggt ctgcgaggtc gagcagctcg ggcgcgggcg ggttgtggct ggtgcggacg
      121 gcgttgacgc ccatctcttt cagaatctcg aactggcgtt cgagcgcgcg cgtgttgatg
      181 gccgagccga gcgcgccgag gtcgtggtgc tggcagacgc cgttcatccg caccggctcg
      241 ccgttgagca ggaagccgcg cgcggcgtcg aacttgatgg tgcggatgcc gaggacggtc
      301 tcgtaacggt cgatgacctt gccgccgtgc tcgacgctgg tgacggcggc gtaaaggttg
      361 ggctgccgca cgccccacag cttcgggttg cggacgcgcg cgctcgtgcc gacggtcgcg
      421 cgctgcccgg cggcgacgtt gagaccggcg ggcgcgaagg tggccgcggg cttcgcgcgc
      481 ctccgaccgt cggcgccgag ttcgtaaagc tccgtcctca aggtcacgtt cgcggcggcg
      541 ttcgacctgt tatcaacggt cactttaaga tcgacggtcg cggcctcgct cgtgacttcg
      601 ggcgtcgtcg cgtaggtgcc ccagtggccg acgtggacgg gcgcggtctt gacgagccag
      661 acgttgcggt agaggcccgc gccgggatac cagcgcgaag actcgggcgg gttgtcgagg
      721 cgaatcgcca gcacgttctc cgcgccgtac tcgacgtagg gcgtgaggtc gaggcggaac
      781 gacgcatagc cgtagggcca gccgccgacg agctggccgt tgagccagac cgccgagtaa
      841 gccatcgcgc cgtcgatgtc gaggtagagt tgtcgcccgc gttcgcccgc cgcgacgtgg
      901 aagcgcttgc gataccagcc gacgcccgcc caggggagct tgcccgtctc gcccgacagc
      961 gcctggtcga aaggcccttc gatgccccag tcgtgcggca ggttcagacg ccgccacgcg
     1021 ccgtcgtcga agccgcgccg cgtgtaggcc acgtcctcgc cgacgttccc ctcggggcgc
     1081 gtccttcccg acccgcccgc gacgaactcg ccgccggtcg cgttgatcca gcccttgatc
     1141 ctctcgtaga caagcgcccc ttcggcgccc gccgggtcgc gcctctgaaa ccgccagtcg
     1201 gcgttgaagg atgcgcgctc gcgcgcgggc tgcgccgatg cgtcgggcac acacaaggcg
     1261 aacacgaagc agagacaaaa gaggtaggcg acggcgcgcg cgccgcgtcg gcgaatcgag
     1321 tcgtggatgg tcgtcatggc tttaggtgcg tgcgagcggg aagggcgact tgatgcggag
     1381 cgtgtcgccc gcgtcttcaa gctcgaagag gcgggcgcgt tcgcgctcct tgttgggctg
     1441 gtgcaggacg agcatgagct ggccgtcgaa gcgcttgaag atcatgccgt gcccgccgtc
     1501 ttcggcgaac agcggcgcgg cctgttggac ccacgggccg cgcaagcgtc cggaggtcga
     1561 gcgggcgacg ccgaccgtgt agccgccctc gccgaagctc gaccagatca tcaggagctt
     1621 gccggtgcgc gagcggtaaa ggtaaggcgc gtccgtcacc cagcagcccc actgcggcga
     1681 cttgcggccc cacggcgcgt cgctcccggc gaacagtttg gtcggctcgc ccaccgtctc
     1741 cgagaggtcg tctgtcagac gaatcatctc gatggtgccg tcggtgatct gcacccactc
     1801 gtgcgcgtag accatgtagg gcgcgccgtc ctcgacccac agcgtcgcgt ccagcgtcat
     1861 catgtccggg gggagcgtcg agcggttccg gaaaggcttg aaggggccga gcggcgagtc
     1921 ggcgaccagc acctgcgagc cgcgcttcac gcggggcagc cagttgcgcc actgctccgg
     1981 gaagagcgag tcggtgtcga aggtgaggaa gagataatac ctgtccttgt aggcgatcag
     2041 ctcgggcgcc cagatgccct tgatgtcggc gtcgccccag aggccgcgcg gggtctggaa
     2101 aatgatgtgc ggcccttccc acgtcacaag gtctttgctc ttgtaggcgc gcaccgcggg
     2161 gacgcgggca tcggcgaccg gcacgcgcgt ggacgacacc gcgtagtaag tcttcgtggc
     2221 ctcgtccgca aggatgcaca cgtcgcgcat ccgcacatcg gcgagacgca cagggccttt
     2281 gccttgagtg gacgaggctg gcgtcgtctg tgcggcgggg gcggcgccga gcattaagca
     2341 gcagaggccg aagatgattt tggtcgcgtt catgtgtgtc gcttgaaaga tgtcgttctg
     2401 ttgcggctcc gccgcctgct gtgcggaaag gctgtgcctt tccgatcacg gtcgcgttga
     2461 aattatgcag gggctacgcc cccgcgctga ggcgcagcct cagccaaact aaacgagtac
     2521 gtcgccggtg aggcgaagcc tcaccgcaca tcaggcggca gagccgcaac ggaaccatac
     2581 tttccggctc ttacttcgaa ctcactttac ttcactgctt accagtaatg gtgcacgagc
     2641 gggcggatgc gccgttcgta aagctctcgg acggcgcgca tcgtctcttc cgagaggggc
     2701 gcgaggtcgg cggcgcggac gttgtcttcg gcctgcgagg ggcgcttggc gccggggatg
     2761 gcgcaggtca cggcgtcgaa catcagtatc cagcgcagcg cgaactgcgc catcgtcgcg
     2821 ccttctggca cgagcgcgcg cagctcttcg acggcctgaa ggccggtctc gtaatcgacg
     2881 ccggagaagg tctcgccgcg gtcgaaggcg gcgccctcgc ggttgaaggc gcggtggtct
     2941 tccgcggcga accgcgtctc gcgcgtgagc ttgcccgtca gcatccccga agagagcggc
     3001 aggcgcgcga ggatgccgac gcggcggcgc ttcgcctccg ggaagaaaag gtcggcgggg
     3061 cgctggcgga agatgttgaa gatgatctgc accgtctgga cgttcgggta ctcgatggcc
     3121 ttcagagcct cttcgacctt ctcgacgctg acgccgtagt ggcgaatctt gccctgctcg
     3181 accagccggt cgagcgcgcc gaacagctcg gggaagtaat agacctcggt cggcgggcag
     3241 tggagctgca cgaggtcgag cgcgtcggtt tccaggtttt gcaggctgcg ctcgatgaag
     3301 cccgcgaggt tctccgccgt gtagccggcg gcgacgtgcg ggtcgaggcg gcggcccgcc
     3361 ttggtggcga tgatgatctc ttcgtcgcgc tcgcgccgga gccgcgcgag caggcgctcg
     3421 ctgcgcccgt cgccgtacac gtccgccgtg tcgaagaagt tgacgccgag gtcgaccgcc
     3481 ctgtgcagcg cggccaaaga ctccgcgtcc tcgacctcgc cccacgtgcc gccgatggcc
     3541 cacgccccga aaccgatctc cgagacgctg tagcccgtgc gcccgagttc gcgataattc
     3601 atccctctgc cctccctgtt tgacgttgac gcgccgcgag attcagcgcg cggcgaagtt
     3661 gtagaggcgg agccagtcgg cgcagcgcgc gggccacgtg gcgaagacgg ggtcggcgct
     3721 gccctcgcgc gtgccgagtc cgaggccgtg gcgcccgcgc tcgtagaggt gcatctcgaa
     3781 ggcgacgccc gcgcgcctga gcgccgacgc gaacatcatg ctgttctcga ccggcacgcc
     3841 cgcgtcgttc acggtgtgga cgatgaaggt cggcggcgtc tctttcgtca cctgctcttc
     3901 gttcgagagc agcgcgacca gctcctcttt cggcgcgtcg ccgagcaggt tctttttgga
     3961 accggcgtgc gtcagctccc tcatcgtgat gacggggtag atgagcatga gcaagtcggg
     4021 gcgcgagctg acgcgttcga tcttgtcggg ggcgtccgat ttgcccgcgt cgaagtgcgt
     4081 gccggcggtc gaggcgacgt ggcccccggc ggagaagccg aggatgccga cgcgcgcggg
     4141 gtcgaggccc cactcggcgg cgcgtgcgcg cacggtgcgg atggcgcgcg ccgcatcgag
     4201 cagcggcgcc gggtggttgt aacgcggccc gagccggtac ttgaggacga aggccgtgac
     4261 gccgagggtg ttgaaccact cggcgacggg tcggccctcg tgctcggcga gacgcgagta
     4321 gccgccgccg gggcagacga tgacggcggc gcccgtgcgc ttctccttcg gcggccagta
     4381 gggcgtcagc gtcggcgtgt cctgcggctc gcgccctcgc gcgccgggtg cgccttcggg
     4441 ccagagcacg attgtttgct ggtcggttgt ccccacggtc tgtgccgacg gccccgtcgc
     4501 gcagacggcg aagccgacga tgagaagcga cgcccacacc ccggcgcgca caagctttcc
     4561 gctcattttt gtctccgatt tacttgatgg acgagtttag cgcacgtagc gggttaacac
     4621 agagggcacg gaggattcac agagcgcaca gagaagacca tttgagattt gagatttcaa
     4681 atcttgtctt tctgctccgt gctctctgtg tctcctctgt gccctctgtg ttgaatcctc
     4741 actttcgata aactcgacct actgcttgtt gaaggccagc gtggacttca tctcgccgcg
     4801 cggcgtttcg ctcgtgcggt tgatggtgag cgtcttgccg tcggcggcca actcccagcg
     4861 ctctttgctc gtggacgagc gctgctcgcc gtcgcggttg gtgaacttgc gcacgttcgt
     4921 cagctccaaa gccttgccgt cgtccttcca ctcggccttc tgcgtcgagg tgccgcggtc
     4981 gccccaatcc atgctcgtct ccgaaccgtc gagcttgtac gtcaacgagg tcggcatccc
     5041 cctgcccatg ccgccgcgcc cgcgtccctg acccatgccg ccacccccgc cgccggggcc
     5101 gccctgtcct ccggcgccgc tcgcctgacc gccgccgggg ccgccgccct ccttccagtt
     5161 gatcttgtta tcaaccgtga gctgcttctc gtcctgcgag acggtcagcg tgtagctttc
     5221 gaggttgtcc gcccactgct gcggcaagtc cttgctcttc gacttgtcga gcacccaagt
     5281 gcccgcgaag ttggtcggcg cggcggcgct cgcggccacc gcgaacagca gcgcgcaggc
     5341 cagactgcat cccactaaaa accttcttgt catctctccc tcctcggtaa gtgaaattgg
     5401 atcggcgcgg acgccgcagg ccgtgtttgg ccgacgctcg tgcgtgccgc cgaaaccctc
     5461 tcggccccgg agcgacgtaa gaccgcctac ggaagccgtg tcgtctttac ctttaacgac
     5521 gggccgcgga cgcgcgcggt tacaactaat gcacgcgcgc gccgtcgtcc gcgtcagggg
     5581 cgcgccctcg ttcggcggcg acgtggcgcg ggccttcgcg tcgtgcgcgc ggacggcggc
     5641 gcgggcctgt cggcggcgtc ggtgcccgcg tcgatgtcgg tcgtggcggt acgcccgcgg
     5701 cggtattcga cgccggtcac gtccgtgtct tccgccagcc gcggccccgc gtagacgacg
     5761 gctagctcgg cgagcgcggc ggtcgtgtcg gggccgaagc cggagagcgc ggtgagccgg
     5821 aggtggcgcg cgttgacggt cgcggggaag cgtagctgct gcggcgcgaa ggtcgagccg
     5881 agccggccgc gcagcagctc gcgccacgtc gcgccgtcgt cgctcgcgtg gacggcgtag
     5941 tcgcggatgt cgccctcgtg ctcgcggtgg ttctgtcgcg gcatgaggac gaggccggag
     6001 atggccaccg gggcggggaa ggtgacggtc agctcgtgcg ggtgcttcaa gtctctgttg
     6061 cgcgcgtcgc cgacgagcca gtaggtgttg gggtcgccgt cgatggcgtt ggcggcgtcg
     6121 ttcgaggctt gcgcgttcgc ctgcgccacc gcgccgagcc gccgcatgat gcgcgtgtcg
     6181 aacatgagcg cacggatgtc cgagggcgcg agcgcgaccg cggggcggaa gcgcgcggag
     6241 gccatgtagt cgaggagcga gcggcgcaag gtgcgcgcga ccgggcgctc gtcgagcgcg
     6301 ctttcgaggt cgaaggcgga gacgagcagg cgaccgcgcc cgaccctcgc ttcgaagacg
     6361 aggccgagcc ggtagttgcg gttccagtcg tcgatggcgt agacgacggg gcgcagctcg
     6421 cgcggcagcc gatccatgtt caccgcgcgc acgttgcgga tgatctcggc ccactgccag
     6481 tcgaagtggg cctccgtggg gaagcccgcg agcgcggggt ggcgcgtgtc gttccaggtg
     6541 ccgagcatgc ggccccagag cggccccatc agcgcgttcc agaagacggg cacgcggtcg
     6601 agcggcggac tcgtccagtc gagcgccgcg ggcgcgggca cgtacagcac cttgccgcct
     6661 tgagagagcc gcgcctcggc ctcgtcccac gatttggtga cgagcacgtc gcgcggcgcc
     6721 gccgtgttcg cttgcgaagg gtagagccag aagtcccagt cgttctcgaa gacggtgccg
     6781 ggcaggccga cgacgagctt gtaggcggcg ggcgctttga gcctcgacaa atccgccgtg
     6841 accttgccga gccgcgtgtt gccgtgcgcc agtgtgcgcg cgggccacgc gccgcgggcg
     6901 accacgcggc cccccgcgtc cacgatcctc catgaagaag acgctgcttc gagcggcgcc
     6961 gggccgtagt gcgcgacttc gacctctacg tcgagggttt cgtcgctcgt gaagacgcgt
     7021 ttcgtgaggc gcgcgagcgg gacggtggtg ttgcagaagc ggcggaactc ctcgggcgtg
     7081 gcgtagcctt tggactcgaa gaaggcgtcg agcagcccga cgagcgcggt gccttgcccg
     7141 aggtagtcgc gcagatcgag cagttggaag ccggccatgc cgggcgtgcg caggttggcc
     7201 tcgatctcct ccttgtagca ttcgagctgg tacttgcccg aagcccacgc gaagtccttg
     7261 ttcttgtcga gcagcccctt ggccttcatc gagtcgcgga agatttcgta gttgccgggg
     7321 cgcatgtagc cggtgaactt tttgatgatg tcgtagtctg gataggcgac ccactggccg
     7381 agttcgtgcg aggccacggg aacgtctatc ccttcgagcg aggcgcggta gtcgcgtccg
     7441 aaccaggcgg tctggccgcg cagccacttc gggccgatgc gctgcatgac gaggaagtct
     7501 gcgccctcgg tcgggttcgg tatctcgcgc tcggtgtggc cggtgcccgt ggtgtagagg
     7561 cggcgcgggt ctcgctgtcg gaagtgctcg gcccagcgcg ggagcgcttc cttccagcgg
     7621 gccttcggct cgttgctcgc cgagaggagc acgaacgaag ggtggttgcc gtaggctttg
     7681 atcatccgtt cggtctcttc gtagagcatc ttttccatcg gcgtgccggg gctgatctcg
     7741 ttccacatgc ccggctcgga ctggatgtag aagcccagct cgtccgccgc gacgtaggcc
     7801 gcctcgggcg gcgcccacga gtggaagcgg aggtgattga ggccccactc cttgcaggtg
     7861 cggatgagac gccgccacga ttcaacgtcg gtcggaggat accccgtgag cgggaagtcg
     7921 ccgccgtggt gcgtgccgcg caggtgcgtt ttgcgaccgt tgacgagaaa gtcggtgccg
     7981 cgcgcgcgga actccgtgag gccgaaagcg agttcgcgcc ggtcgtcggc cccttcgcct
     8041 tcgagtcgca ggtcgaggcg gtggacgacc gggttgaact cgtcccacgt ccccgcgttc
     8101 gggccgagtt gaacttcaag ctcggccctg ccgccctgcg cgtcccaagt gacgggcacg
     8161 ctcgcgcccg cggcggagag cgtgccgcga ccggcgcgcc cgctcgcgtt gccgatggcg
     8221 actttgatca gcgcagactt cttccgtacg tcggggaaga cctgcgcgtc ttcgatgaag
     8281 accggcgtgg tcgagcttaa atcgatccgc ccgacgatgc cgttccaact gctcccgccc
     8341 gaatccgaga cgctgtgcgc gtccggtcgg taggggagca gcatccggct atcgacgcgg
     8401 acggtgagcg tgtgcctgcc gggcgcgagc gcgccgaggt cgtacgcgtg cggcgccgag
     8461 aggctgttgt tcgcgccgac ctgtctttcg ccgacccaca cggtggactc ccagcggggc
     8521 cgctccatga agagcacgac gcggcggcca cgccactcgg cggggatgtc gatgtcgcgc
     8581 tgataccacg ccgcgccgac gtagtggcgc gggggctgcg agaggaacgg caccttgacg
     8641 cggcccggcg cggtgtacgc cttgaagtcc tcgcgcatga accactgccg gtcgtagagc
     8701 gacaagaccc acggcgtctc ggtgccgatc tcgtcgccgt agccttgcga ctggagcacg
     8761 ccgggcagct tgatgcgctc ggctaaaccg cggctgaacc agcgctcgcg caccccctga
     8821 tcggcgcggt cgagcgcgaa accccactcg cccgcgagcg ggacgcgccc ggccggctgc
     8881 gccgccgcgg tcgccgtcca gcacgccgcc agcgccagca gcaggagccg cgcggcggcg
     8941 gcgtgtacgt ttctgctcat gcccttcaac ctttccgtaa gcccggcggg ttcgccacaa
     9001 aaataggtca tctggtgccg aaataaccgt tgacttggcc tgcaaagtgg catagaagta
     9061 agcaactcac cagcataggc acccacgcgc cgcccctcgc ataagcggca gcgtcaacag
     9121 ctcagaggag tcgtcaaaat gagattaaag cgtagtgcct cgttcgtcgt tctgccttcg
     9181 attctactct gcctgctccc cgccgggctt ctcgccgccg ccgacaaagg ggcggcgcat
     9241 cacgtccccg ccgcacgcga gcgctggggc gactcgccgc accacgcggg cgtcatcagc
     9301 taccacggcg gggcgagcgc cccgaccgac gcgcgccgcg ccgccgccgg tcggcgcgac
     9361 cgcaagggcg gcatccgcga ggtcgtgccc gacaagtaca agggccgtta tcaggagtgg
     9421 aagaaggagt tcctctccac cgagaccggg cagcagcagt gggcgctgtt cgagaagagc
     9481 gaacagctca cgctgacgat ccaggtctcg cgcgacaacc cgaagggcgc gggcacgggg
     9541 cagtacaagt gggacgacga aggcaagctc gtcgaggcga ccatcgtcct cggctccagc
     9601 ctccacgagg gctacccgaa cccgatctac taccccgtga tgaactcgct gatgcagcag
     9661 gagccgacct acgcgggcag cggccacacg ctcgccgcca ccaagatcgc ccacgagttc
     9721 ggccacctca cgcgcacggt cgagatggat cacgcgctct acgagctaca gaaccgcctg
     9781 atgccggtct acaacaaaat cctgctgagc aatgggcgca acacgagcga cccgcaactg
     9841 gtcgagctgg cccagcagat gggcggcacg ccggtcgaaa tctgggaaga ccgcgaatac
     9901 tggggcgagg ccaacgccat gctcttcctc cgcgaccgct tcgccgaaga agacctcggc
     9961 tgctcgctct tcggcaaaat caagcagagc gtcaacctct acgccaaaaa ctacaccgag
    10021 cgcttcctca aggtcgcgca gtcgtcgccc tcgctcaacc gctgcggctg gcgctgacga
    10081 ttgcggattt cggattgcgg aatgcggatt gaagaaccga cgacgtttga tggaagcgct
    10141 gcttccatca accgtcgtcg cgcttctagt ctgcggttat cgcttgtcga atccgcaatc
    10201 cgaaatccgc attccgcaat cgttcagagt tgtcgcagca gcgccgcgtt caagccttcc
    10261 cagcggactt ggaaggagcg cgcggggaag ccgggcgcgt gcgtgcgcgg cgcgccgcgc
    10321 cagccggcgg ccatcagcgc gacggcgtag agcagcccgc cgttgccggg caggtagcac
    10381 ggcaggttcg ggcgctggta gttgtggccg ttcgccgagt agttgttctt cgcggtcttc
    10441 aggagcagcg cgtcgatggc cgtctcggtc tcgccgagtc gcgccgccgt catcgccgtc
    10501 atcggatagt cccaccccca cgtctcgtcc cagcgccatt ccttcatcac cttgcgaagc
    10561 gtgcgccgca tcgtctcgcg gtcaacgccg tcgccgtcca agacgccgag cgcgccgagc
    10621 atcgaggggt ggtcgtggtt gcgctcggtg taggtctgcg ggcagttctc gtgcgcgagg
    10681 tagacgcctt cgcgcgccgg gagcggcgcg aggcgcgaga gcactcgatc ccacttcgcg
    10741 tcgcgtttca tcccgagacg ttcgcgccag ctctgcgccg tcttcagacc gtaaatccag
    10801 taggccagct cgaaggtcgg gttccaggtc tcgcgcggcg gatggttctc ctgcgcgggg
    10861 atgacgggcg ggccgagcac gtagcgcccg cgctgctcgt cgaagaaggc gaaggaggcc
    10921 atgaactccg ccgtctcgaa gacgacgcgc cggtagcgtt cgagcgcgcg gcgaccgccg
    10981 cctgcgaggt aacaaagctc cgcataaaat atcgggtgcg gctgctgcca gatgagcagc
    11041 ggaccgatgg gcgaggggct gtcgcgcccc tccggcccga ccatcttcgg ccagcgcgcg
    11101 cccgcgtagc cttgcgaacg cgccagagcg cgcgcccccg gcaaaacctt gtcgtaccag
    11161 ccgaggctct tctcaagcag agggagcctg ccccacagag ggaagtgcgc ggcgtgccac
    11221 caatgcatct caaggtgaaa cttgccgtac caactgttgg tcgtcagtcc tgtctcctgc
    11281 ggcggctgcg accccgcgca ctgtatcgcc gtgaggtact gcgagagcac gacgcggcgt
    11341 tcgagttcgt gcgcgcgcgg gtcgcggctg ccggccagct cgatcgcgcc gcccgtcgtc
    11401 cagaagcgct gccagtgggc ggcggcggcg gcgaacgtgg cgcgcgcggt cgggagcatt
    11461 tgggcggcgg ggcgcggcgc gaagtgtgcg acgaattcga gacggcgggc gcgcgccgcg
    11521 ggcgtcagta gaaagtggtg ctccttctct gcggcgagcg tcgcgggcgt gtgccacgcg
    11581 agcgcgaccg agtagcggtc ttcgtagagc gcgcggcgaa tctcggcgcg gctgacggag
    11641 cgcgcggcga gtacggagct gtgcctcgcg ggctgccccc agtcggcggc gtgcatcttc
    11701 ggcgagccgt aagggaaggc gaggcgcacg gcgaggcgcc cctcttcgat caaagacgat
    11761 tcgacggcga cggccagcag gtcgagccgc gggtggacgg aagtcctgac ggtgacgggc
    11821 cgcccgtcga acctgaaacg gctcgtgatg gtgcccgacc agaggtcgag gcgctgctcg
    11881 atgtccgtga tgtcgccggg gcgcgcctct gtgccgtcgc ttcgcgtcag gcgcagaccg
    11941 agctggccga ggtgcaggcg gtgcgggttc tcgcgcagcc agtcgaaaag ctcggcctga
    12001 ttttcggagc tggtctggta gccgacctgc cgcccgtgcg tgtcgtactg gacgaggcgg
    12061 agcgcggcgc cctccaggct ctgcgggcgc gggctggtgt gccagcccca ctgcgacatc
    12121 gtgcagagcg gcatcgcgtt ctcgtactcg ccggggaagg tctgtagccc cgtcacgtcg
    12181 cccgtgaagg cgaactcgcc gttgccgaca gagagcggcg agagcgggtc gagcttccgc
    12241 aaggtcgggc tgtgacggtt ggcgagcgcg gcccggtcga tcacgccggc ccctctctga
    12301 gccgcggcgg gcgcggacag ggccgagccg tagaggccgc cggaggcgac gagcgttcct
    12361 aaaaattcac gacgcgaaac cataggttca atttcgaaga aggggaaaag gttaagaggg
    12421 gaagaggcga aaaggtgttt tgccttcacc cttttcccct cttgcccttt cccccctttt
    12481 acgcgaaggc cggttaagaa agatgggatt ggatgacctg gtgcgggtca ttcaatccca
    12541 tcttggaaga gccgagctgc gttcttcagg cttagaagag gaacttcacg ccgagctgcg
    12601 cgcggcgggc gtagttctcc tggttcgagg cgctgatctg cccgaagttg gaggccgtct
    12661 ggttgacgac cgggttgggg aagtaggcct cgttgaaggc gttgatgagt tccatcctca
    12721 actggacgcg catgccctcg cggatgtgga tgtccttgag aagcgagaag tccacgttgt
    12781 tgatggagtc gcggcgcacg ctgctgaagc ggtacggcaa ggtgcgcagg tggttgaccg
    12841 gggccgcgtt cgtcgaggtg gcgttggcca gcgaggtgaa cgccgccgtg ttgaaccacc
    12901 gttcggtggt cgggttgtca atcgcgatct cgccgccgtt atagaagagg tcgccggcgg
    12961 tgttgccgtt gcccgtgttg aacgagccga aggggatcgg gaagccgctc tggtaggcgt
    13021 agacgccctg aatctgccag ccgccgacga tggcgttcgt gagccagccg gcgtcggtca
    13081 gccaccgctg attcttcccg aacgggaagg cgtacatcgc gctcagagag aggcggtgcg
    13141 gcacgtcctg gtcggagatc atcttggtcg gcgcggcgtc gcccgcgttc aggtactcgg
    13201 tctcctggag ccacttcgac cacgtgtagc tggcttgcag cgtgtagttg cgcgtgaacc
    13261 gcttttcgag gcggaactgg cccgagtgat accacgactt gccgtcgttg acggtcgtgg
    13321 tgatgtcgcc gaaggccggg aaggggcgca agagctgcga gcgcgcgatg gtcccggcct
    13381 ggagtccgga acccgccggg aacagcccca cgaacgggtt ggcaatcgtg ccggtcagga
    13441 acgtgttgtt ggcgttcatc gcggcggtgc gcgagttgtc ggtgttcagg tactggttcg
    13501 ggagcgcgtt gatgttgcgc gtgatctcga tgttgtagcc gtagttgccg acgtagacgg
    13561 cctcggcgag ccagccgccg ggcagctcgc gctggaggcc gatctgccag cgcgcctgct
    13621 tcgagacctc ggggttctgg ttgaagaacg agatggcgcc gccgaggccc gtctggcgcc
    13681 cctgggcgtt gccgaccggc tcctggacga cgatgttggc gaagttggcg atgttgaccg
    13741 ggatcggcgc gccgttggcg ttggtgatcg tgctgatctg cgtggtgcgc gtgtagc
//
LOCUS       DBNGOH010000899         3758 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2335380, whole genome shotgun sequence.
ACCESSION   DBNGOH010000899 DBNGOH010000000
VERSION     DBNGOH010000899.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3758)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3758)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3758
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2335380"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..265)
                     /locus_tag="AC159P_17515"
     CDS             complement(<1..265)
                     /locus_tag="AC159P_17515"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024304.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HIR3697294.1"
                     /translation="MRNGERWIENQLSPSRSSFRIPHSSFIFRGIVMLEIKDMPRGEM
                     LALLLRVGYGHLGCARDGHPYVVPMHYAFDSQDIYFFTTEGTKT"
     gene            complement(276..1397)
                     /locus_tag="AC159P_17520"
     CDS             complement(276..1397)
                     /locus_tag="AC159P_17520"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003954 - NADH dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006116 - obsolete NADH oxidation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR3697295.1"
                     /translation="MANILILGGGFGGVVAAEKLAKTLAPEHRITLVSRSRRFTFYPA
                     LVRLAFGKCEEEDISYDLGDAMLDRRVRFIEAEVTRVDPRARKVSLSGGDIVGDIKYD
                     YLVYALGRRLATEQVKGFFEHAHHLLGVKAARRFGEAARNFRGGHAVLGSCPGARLDV
                     PVYEAAFALARQLAEQGRDARITVISPEYPSARPGGADLVRALKPALAAHHIEHLTDF
                     PIAEVTAEAVVAGDGREVEYDLLMLVPPFEGTSALANTGLTDEEGYVRVNDTMRSLRA
                     DRMYAVGDAVYFSGPKMGHMAVRQAETAAANLAAEIEGAGTVAHYDHEITLVVDEGGK
                     DSVFLHQKLWETGEQSIRHGRFWSWAKRAHEKYFRAQHS"
     gene            complement(1448..1921)
                     /locus_tag="AC159P_17525"
     CDS             complement(1448..1921)
                     /locus_tag="AC159P_17525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010940934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR3697296.1"
                     /translation="MKILLATDGSPCSDSAVKEVARRPWPADTQVRIVSVAEQPAPLV
                     AEPYMVPSAYFEEAAKAIRVQAEQAVERAAATLREARNGGLPVSTEVLNGSPKRTIVE
                     EAEAWGADLIVVGSHGYKSWERMLLGSVSQTVAAHAECSVEIVRCAESDERRVTS"
     gene            complement(2107..>3758)
                     /locus_tag="AC159P_17530"
     CDS             complement(2107..>3758)
                     /locus_tag="AC159P_17530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015189071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3697297.1"
                     /translation="NRAKDGSLYWVDTTIVPFLDARGKPYQYVAIRYDITERKRAESR
                     IREQAALLDKARDAIVVRDTRDNKILYWNKGAERLYGWTEGEAVGRTAGELQLLNESN
                     AAQFERANQSVMKTGEWAGELRQRTKGGVEIIVESRWTLLREDGGEPRSILVINTDVT
                     ERKKVEAQLLRAQRMESIGTLAGGIAHDLNNILAPILMAVQMLRLKVDDPAALQWLEI
                     LQANSERGADMVRQVLSFARGVEGERVTLQPKHLVKEVIKILKDTLPKSIEIKYDVPA
                     DLWTVSADATQVHQVLMNLCVNARDAMPRGGELSIRAENVTLDEAYARMHLEAEPGRF
                     VSVTVADTGEGMTPDVLARVFEPFFTTKEVGKGTGLGLSTALTIVRSHGGFISPYSEP
                     RKGTRFSVYLPAVEAAEAERAERRRLDLPEGRGELVLVVDDEEAIRQITKGTLETFNY
                     RVLVASDGTEAVALYAQHREEVAVVLTDMMMPYMDGPATIRALQKMNPRVRVIAASGL
                     SVGDKAAEAAAVGAHAFLAKPYTAEKLLKALAEILRAPEKD"
BASE COUNT          566 a         1282 c         1212 g          698 t
ORIGIN      
        1 cggtcttcgt cccttcggtc gtgaagaagt agatgtcctg cgagtcgaag gcgtagtgca
       61 tcggcacgac gtaagggtgt ccgtcgcgcg cgcaccccaa atgtccgtag cccacgcgca
      121 agagcaacgc cagcatctcg ccccgcggca tatctttgat ttccagcatc acgatacccc
      181 gaaaaataaa tgatgaatgc ggaatgcgga atgatgatcg agaaggcgag agctgatttt
      241 caatccatcg ttcaccattc cgcattcatc gtttcttatg agtgttgcgc ccggaaatac
      301 ttctcgtggg cgcgcttggc ccagctccag aagcgcccgt ggcggatgct ctgctcgccc
      361 gtctcccaga gcttctggtg aaggaagacc gagtccttgc cgccctcgtc aacgacgagt
      421 gtgatctcgt ggtcgtagtg cgcgaccgtc cccgcgcctt cgatctccgc cgcgaggttg
      481 gcggctgccg tctctgcctg ccgcaccgcc atgtgcccca tcttcggccc cgaaaagtag
      541 accgcgtcgc cgacggcgta catcctgtcc gccctgagcg agcgcatcgt gtcgttcacg
      601 cggacgtagc cctcttcgtc ggtcaggccg gtattggcaa gagcactcgt tccctcgaag
      661 ggcgggacga gcatcagcag gtcgtactcg acctcgcgcc cgtcgcccgc aacgaccgct
      721 tccgccgtca cctcggcaat ggggaagtcc gtgaggtgct cgatgtggtg cgccgcgagc
      781 gcgggcttga gggcacgcac gaggtctgcg ccgccgggcc gcgcgctcgg atattcggga
      841 ctgatgacgg tgatgcgcgc gtcgcggccc tgctccgcga gttggcgggc gagggcgaag
      901 gctgcctcgt agaccggaac atcgagacgc gcgccggggc acgagccgag gacggcgtgc
      961 ccgccgcgga agttgcgcgc cgcctcgccg aacctgcgcg ccgctttgac gccgagcagg
     1021 tggtgcgcgt gctcgaagaa gcccttcacc tgttcggtgg cgagccgccg cccgagcgcg
     1081 tagacgaggt agtcgtactt aatgtcgccg acgatgtcgc ccccgctgag cgaaaccttg
     1141 cgcgcgcgcg ggtcaacgcg cgtgacctcg gcctcgatga agcggacgcg gcggtcgagc
     1201 atcgcgtcgc cgaggtcgta cgagatgtcc tcctcctcgc acttgccgaa ggcgagccgc
     1261 acgagcgccg ggtagaaggt gaagcggcgg ctgcgcgaga cgagcgtgat ccggtgctcc
     1321 ggcgcgagcg tctttgccaa cttctccgcc gccaccacgc cgccgaaccc gccgccgaga
     1381 atgagaatgt tcgccataat tactccccta gtgacgagtg atgagtgatg agtttggaac
     1441 ccctatctta gcttgttact cgtcgctcgt cactttctgc acaacgcacg atctcgaccg
     1501 agcactcggc gtgagccgcc accgtctgcg agaccgagcc gagcagcatc cgctcccaag
     1561 acttgtagcc gtgcgagccg acgacgatga ggtcggcgcc ccacgcctcg gcctcttcga
     1621 cgatggtgcg cttgggcgag ccgttcagca cctcggttga aactggcaag ccgccgttgc
     1681 gggcttcccg cagtgtcgcg gcagcccgct cgaccgcctg ttcggcttgt accctgattg
     1741 ccttcgctgc ctcctcgaaa tatgcactcg gaaccatgta cggctcggcg acgagcggcg
     1801 cgggctgctc ggcgaccgac acgatacgca cctgcgtgtc cgcgggccac ggcctgcgcg
     1861 cgacctcttt gacggcgctg tcgctgcacg gcgagccgtc agtagctagt agtatcttca
     1921 tcgcggttac tcctctttgg aagggactcg ttccctcatc ttcgcggttc gcttcggcaa
     1981 cgtgtgtgcc gcgcggtttc acgccgattt gcggcgccag aaaaggcgcg gcggcgctga
     2041 atcgcggaat attacccgaa gcggttgcgt aaattttcgt gggggggaga tgagattgga
     2101 gcggccttaa tccttttcgg gggcgcgcag gatttcggcc agcgccttga ggagtttctc
     2161 cgccgtgtac ggcttcgcca ggaaggcgtg cgcgcccacc gccgccgcct ccgcggcctt
     2221 gtcgccgacc gacagcccgc tcgccgcgat gacgcgcacc cgcgggttca tcttttgcag
     2281 cgcccggatc gtcgccggcc cgtccatgta cggcatcatc atgtccgtga gcacgaccgc
     2341 cacctcctcg cggtgctgcg cgtagagcgc gaccgcctcc gtcccgtcgc tcgccacgag
     2401 cacgcggtag ttgaaggttt ccagcgtccc cttcgtgatc tgccggatgg cctcctcgtc
     2461 gtccacgacc aacaccagct cgccgcgccc ctccggcagg tcgagccgcc tccgctcggc
     2521 gcgctccgcc tccgccgctt cgacagccgg caggtagacc gaaaaccgcg tccccttgcg
     2581 cggctcgctg tacgggctga tgaaaccgcc gtggctgcgg acgatcgtca gcgccgtcga
     2641 gagaccgagg cccgtcccct tccccacctc cttcgtcgtg aagaacggct cgaagacgcg
     2701 ggcgagcacg tcgggcgtca tcccctcgcc cgtgtcggcc accgtcaccg agacgaaccg
     2761 gcccggctcg gcttcgaggt gcatgcgcgc gtaagcttcg tcgagcgtga cgttctcggc
     2821 gcggatggaa agctcgccgc cgcgcggcat cgcgtcgcgc gcgttgacgc agaggttcat
     2881 caggacctgg tgaacctgcg tcgcgtccgc cgacaccgtc cagaggtctg cgggcacgtc
     2941 gtacttgatc tcgatggact tcggcaaggt gtccttgagg atcttgatga cctccttgac
     3001 gaggtgcttc ggctggagcg tgacgcgctc gccctcgacg ccgcgcgcga acgagagcac
     3061 ctgccgcacc atatcggcgc cgcgctcgga gttggcctgg agaatctcca gccactggag
     3121 cgcggcgggg tcgtccacct tgagccggag catctggacg gccatcagga tcggcgcgag
     3181 gatgttgttg aggtcgtgcg cgatgccgcc cgcgagcgtg ccgatggact ccatccgctg
     3241 cgcgcgcaaa agctgcgcct cgaccttctt gcgctcggtc acgtccgtgt tgatgacgag
     3301 gatgctgcgc ggctcgccgc cgtcctccct gagcagtgtc cagcgactct cgacgatgat
     3361 ctcgacgccg cccttcgtgc gctgccgcag ctcgcccgcc cactcgcccg ttttcatgac
     3421 ggactggttg gcgcgctcga actgcgccgc gttcgactcg ttgaggagct gtagctcgcc
     3481 cgccgtgcgg cccacggcct caccttccgt ccagccgtag aggcgctcgg cgcctttgtt
     3541 ccagtagagg attttgttgt cgcgcgtgtc gcgcacgacg atggcgtcgc gcgccttgtc
     3601 gagcagcgcg gcctgctcgc ggatgcgcga ctcggcgcgc ttgcgttcgg tgatgtcgta
     3661 gcggatggcg acgtactggt agggcttgcc ccgcgcgtcg aggaacggga cgatggtcgt
     3721 gtccacccag tagagcgagc cgtccttcgc ccggttgc
//
LOCUS       DBNGOH010000900         4219 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4167648, whole genome shotgun sequence.
ACCESSION   DBNGOH010000900 DBNGOH010000000
VERSION     DBNGOH010000900.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4219)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4219)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4219
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4167648"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="AC159P_17535"
     CDS             <1..225
                     /locus_tag="AC159P_17535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697298.1"
                     /translation="FHAALNDALLARVVGEGTMGLLKMLGHTDEECISHPVLSRAIRG
                     VQEKIARRARGDLPTASAEDWFKYNTGSST"
     gene            277..1677
                     /locus_tag="AC159P_17540"
     CDS             277..1677
                     /locus_tag="AC159P_17540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018382353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3697299.1"
                     /translation="MDERPEGSAEEQPTEVKTRLTPDPVEAAEAGARRERGRARRALG
                     AVAVDLTPLRVSRDFRLLFVAQSVTFFGSMMTFVTIQWQVYELTESPVVVGLLGVAEF
                     VPIMLLAFVGGALADYVDRRRMVRLTELALALGTGLLVLNALLPQPRVWVLFLCAALF
                     AALNGLQRPSLDALVPRLVPAEMLPAAVALRSLGVMVGMIGGPALGGLLAATLGPALA
                     YSIDLVTFAVSLGALWLIRAVPPPVGADRPSLRTIVEGLRYARSRPELLGTYLVDMNA
                     MFFGMPMSLFPAIAKNFGGSSVGLLYAAPAFGSLLATLTSGWTKRVHRHGLAVCIAAA
                     CWGAAIIAFGLAESLWLALACLALAGAADMVSGLFRSTIWNQTIPDHLRGRLAGIEMV
                     SYTTGPLLGNAEAGLVARLFSVRTSVVSGGVLCVIGTGLLALALPAFLSYDGREGLRR
                     KQEAEEARAEAIEQTV"
     gene            1817..3298
                     /locus_tag="AC159P_17545"
     CDS             1817..3298
                     /locus_tag="AC159P_17545"
                     /inference="COORDINATES: protein motif:HMM:NF016289.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HIR3697300.1"
                     /translation="MSNRRSSRLLSLLVLLALLSQAVARQAPQPSTEIKQAAALAGAV
                     LVSGRSMDYVKGLTDGFGGRLSGSPAYERSAEWAAEQFRAAGIKDVRLEPFTIDNGWQ
                     RGEARGRVVAPVERALNVASLGWSASTPPGGVRGEVVWVGDVSPEFFRKRGGELKGRV
                     AMLDVQSIFARGVWQGFGQLVNSHQRFKDAGALAVVFPDSEANNVLNAFSPSWGARAT
                     PLPYAQIGMEDAKLIRRLSEAPAAKPVTIELQFDNKVSGRTTTHNVVAEIRGRERPDE
                     WVIIGAHLDSWDYGTGAHDNATGCAMVLEAARAIASLPQPPRRSIRFALWGGEEQGLL
                     GSAAYVAAHSAELKDCVAALNTDNGAGHPRGWKVQGREDVSEAMGPISEALLAGLAGG
                     GLSPELTFDTDHGHFMLAGVPALDLWVEMEGYQKVHHKSSDTLDKVEAHDLAAGAAIV
                     AVTAYAVAERETRLAPHDARGAVGERLKKAGLDEFLKAIGVWK"
     gene            3419..>4219
                     /locus_tag="AC159P_17550"
     CDS             3419..>4219
                     /locus_tag="AC159P_17550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4037 domain-containing protein"
                     /protein_id="HIR3697301.1"
                     /translation="MPDFIKGLELSRLFFDEAVRPVLDADFPRLVYAAARLHTGSEVL
                     GFDDETSADHCWGPRVDLFLTDEDYAAARRAVDEALRAKLPHAFRGYPTDFTDPDPTD
                     NGTFRLEFKDAGPVNHKVEITTPRRFFQDYLAFDIGRDIEPADWLTFPEQKLRTVASG
                     AIFHDRIGLEDVRAKFSYYPHDVWLYLLASAWARVGQEEHLMGRAGMVGDELGSALIG
                     ARLVRDLMRLCFLMEKAYAPYPKWFGTAFARLACADALSPHLGAALAAA"
BASE COUNT          620 a         1432 c         1497 g          670 t
ORIGIN      
        1 ttccacgcgg cgctgaacga cgcgctgctc gcgcgcgtcg tcggcgaagg cacgatgggc
       61 ctgctgaaga tgctcggtca cacggacgaa gagtgcatct cgcaccccgt cctcagcagg
      121 gcgatacggg gcgtgcagga gaagatcgcc aggcgcgcgc gcggcgatct gccgacggcg
      181 tccgccgaag actggttcaa gtacaacacg gggtcttcga cgtgagcgac gcgcgagacg
      241 acggagggtt cggcgaacga ggggcgtgag agtccagtgg acgaacggcc agagggttcc
      301 gcggaagagc agccgacgga ggtgaagacg cggctcacgc ccgaccccgt ggaggcggcg
      361 gaggcgggcg cgcgccgcga gcgtggtcgg gcgcgcaggg cgctcggcgc cgtcgcggtg
      421 gacttgacgc cgctgcgcgt gtcgcgcgac ttccggctgc tcttcgtggc gcagtcggtg
      481 acgttcttcg gctcgatgat gacgttcgtc accatccagt ggcaggtgta cgagttgacg
      541 gaatcgccgg tcgtggtcgg gctgctgggc gtcgcggagt tcgtcccgat catgctgttg
      601 gcgttcgtcg ggggggcgct cgcggactac gtggatcggc ggcggatggt gcggctgacg
      661 gagctggcgc tggcgctggg cacggggctg ctcgtcctga acgcgctgct gccgcagccg
      721 cgcgtgtggg tcttgttcct ctgcgccgcg ctgttcgccg cgctgaacgg gttgcagcgg
      781 ccttcgctcg acgcgctcgt gccgcggctc gtcccggcgg agatgttgcc tgcggcggtg
      841 gcgctgcgct cgctcggcgt catggtgggg atgatcggcg ggcctgcgct cggcgggctg
      901 ctggcggcca ctctcggccc ggcgctcgcg tactcgatcg acctcgtgac cttcgccgtc
      961 tcgctcggcg cgctgtggct catccgcgcg gtgccgccgc ccgtcggcgc cgacaggccg
     1021 agcttgcgga caatcgtcga agggctgcgc tacgcgcgga gcaggcccga gctgctgggc
     1081 acgtatctcg tggatatgaa cgcgatgttc ttcgggatgc cgatgtcgct cttcccggcc
     1141 atcgccaaga acttcggcgg gtcttcggtc gggctgctct acgcggcgcc cgccttcggc
     1201 tcgctcctcg ccacgctgac ctcgggctgg acgaagcgcg tgcaccgcca cggcctcgcc
     1261 gtctgcatcg ccgccgcgtg ctggggcgcg gccatcatcg ccttcggcct cgccgaaagc
     1321 ctgtggctgg cgctcgcgtg cctcgcgctc gcgggcgcgg cggacatggt gagcggcctc
     1381 ttccgctcga ccatctggaa ccagaccatc ccggatcatt tgcgcgggcg tctcgcgggc
     1441 atcgagatgg tgagctacac gacggggccg ctcttgggca acgccgaggc gggtctcgtc
     1501 gcgcgtctct tcagcgtgcg cacctcggtc gtctcgggcg gcgtgctctg cgtcatcggc
     1561 accggcctgc tcgcgctcgc gctcccggcc ttcctcagct acgacgggcg cgaaggtctg
     1621 cggcgcaagc aggaagcgga agaggcgcgc gccgaagcga tagagcagac cgtttgagtc
     1681 gggcgccgaa gtcacacccg accgtcacga cgcgagtgac gaaaatccgg aaagcttttc
     1741 ttagcgcctt cgtgtctttg tgataagttc ccttcatcga ccgggaacac tgccctccga
     1801 accggagatt cttatcatgt cgaatagacg aagcagccgt cttctctctc tactcgttct
     1861 gctcgcgctc ctctcgcagg cggtggcgcg gcaggcgccg cagccctcga cggaaatcaa
     1921 gcaggcggcg gcgctagcgg gcgcggtgct ggtgtcgggc cggtcgatgg attacgtgaa
     1981 ggggttgacg gacgggttcg gcgggaggct gtcggggtcg cccgcgtacg agcgctcggc
     2041 ggagtgggcg gcggagcagt tccgcgcggc gggcatcaag gacgtgcggc tggagccgtt
     2101 caccatcgac aacggctggc agcgcggcga ggcgcggggc cgcgtcgtcg cgccggtcga
     2161 gcgcgcgctg aacgtcgcgt cgctcggctg gtctgcttcg acgccgccgg gcggcgtgcg
     2221 cggcgaggtg gtgtgggtcg gcgacgtgtc gccggagttc ttccgcaagc ggggcggcga
     2281 gctgaagggg cgcgtcgcca tgctcgacgt gcagtcgatc ttcgcgcgcg gcgtgtggca
     2341 gggcttcggg cagctcgtca actcgcacca gcggttcaaa gacgcgggcg cgctggcggt
     2401 cgtctttccc gacagcgagg cgaacaacgt gctcaacgcc ttcagcccga gctggggcgc
     2461 gcgcgcgacg ccgctccctt acgcgcagat cgggatggag gacgcgaagc tcatccgccg
     2521 cctctcggaa gcgcccgcgg cgaagcctgt gacaatcgag ttacagttcg acaacaaggt
     2581 gagcggcagg acgacgacgc acaacgtggt ggccgaaatc cgcgggcgcg aaagaccgga
     2641 cgagtgggtc atcatcggcg cgcacctgga ctcttgggac tacggcacgg gtgcgcacga
     2701 caacgcgacg ggctgcgcga tggtcttgga ggcggcgcgc gccatcgcct cgctgccgca
     2761 gccgccgcgc cgctcgatcc gcttcgcgct gtggggcggc gaagagcagg gcctcttagg
     2821 ctcggcggcc tacgtcgcgg cgcactcggc agagctgaag gactgcgtgg ccgcgctcaa
     2881 cacggacaac ggcgcgggcc acccgcgcgg ctggaaggtg caggggcgcg aagacgtgtc
     2941 cgaggcgatg gggccgatca gcgaagcgct gctcgcgggc cttgcgggcg gcggactgtc
     3001 gcccgagctg acgttcgaca cagaccacgg ccacttcatg ctcgcgggcg tccccgcgct
     3061 cgacctgtgg gtcgagatgg agggctacca gaaggtgcac cacaagtcga gcgacacgct
     3121 cgacaaggtc gaggcgcacg acctcgccgc gggcgcggcc atcgtcgccg tcacggccta
     3181 cgccgtcgcc gaaagagaga cgcgcctcgc cccgcacgac gcgcgtggcg ccgtcggcga
     3241 gcggctgaag aaggcgggcc tcgacgaatt cctgaaggcc atcggcgtct ggaagtgaaa
     3301 tggtaggcag caggcagcag gcagtaggca gtggaaaaac aatctcgcgc gtacggccag
     3361 agttatagcg tcaacgtgag taatgaggag ccttgctgcc tgctgcctac ttgaattcat
     3421 gcccgacttc atcaaaggtc ttgagctgag ccgcctcttc ttcgacgagg ccgtgaggcc
     3481 cgtgctcgac gcggacttcc cgcgcctcgt ctacgccgcc gcgcgcctcc acaccggctc
     3541 cgaagtgctc ggcttcgacg acgagacgag cgccgaccac tgctgggggc cgcgcgtcga
     3601 tctcttcctc acagatgagg actacgccgc cgcgcgccgc gccgtggacg aagccctgcg
     3661 cgcgaagctc ccccacgcct tccgcggcta cccgaccgac ttcaccgacc cagaccccac
     3721 ggacaacggc accttccgcc tcgaattcaa agacgccggc cccgtcaacc acaaggtcga
     3781 aatcacgacg ccgcgccgct tcttccaaga ctacctcgcc ttcgacatcg ggcgcgacat
     3841 cgaacccgcc gactggctca ccttccccga gcagaagctc cgcaccgtcg cctccggcgc
     3901 aatcttccac gaccgcatcg gcctcgaaga cgtgcgcgca aaattttcct actacccgca
     3961 cgacgtgtgg ctctacctgc tcgcctcggc gtgggcgcgc gtcgggcagg aggagcatct
     4021 gatgggccgc gccgggatgg tcggcgacga gctaggctcg gccctcatcg gcgcgcgcct
     4081 cgtccgcgac ctcatgcgcc tctgcttcct catggagaaa gcttacgcgc cttaccccaa
     4141 gtggttcggc acggccttcg cacgcctcgc ctgcgccgac gccctctcgc cgcacctcgg
     4201 cgccgcgctc gccgccgcc
//
LOCUS       DBNGOH010000901         7085 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1054259, whole genome shotgun sequence.
ACCESSION   DBNGOH010000901 DBNGOH010000000
VERSION     DBNGOH010000901.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7085)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7085)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7085
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1054259"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            98..1117
                     /gene="rtcA"
                     /locus_tag="AC159P_17555"
     CDS             98..1117
                     /gene="rtcA"
                     /locus_tag="AC159P_17555"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013941535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003963 - RNA-3'-phosphate cyclase
                     activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HIR3697302.1"
                     /translation="MITIDGSFGEGGGQIIRTSLALSLITGKAFRVYNVRAGRAKPGL
                     QRQHLTAVTSAATIGHAKVDGAGLGAKEFTFVPGEVVPGEYTFSIGTAGSTMLVLQAV
                     LPPLMIAGAPSLLVFEGGTHNVHAPPFEFIKKTFLPLVNRTGPNVSVELQRYGFYPPG
                     GGRFNVYVEPAAALRRLDLLERGPVISERARALVVNIPEHIGERELQVVSQQLGWTQD
                     QLELETSRNAVSGGNVLTIEIESEYLTEVFTGIGERGVRAEVIASRAVREAQRYLDIG
                     APVGEHLADQLLIPMALAGGGAYTTGPLTPHTTTNIEVIRKFLPVEIGRTQLTEDVWK
                     VEVES"
     gene            1278..1865
                     /locus_tag="AC159P_17560"
     CDS             1278..1865
                     /locus_tag="AC159P_17560"
                     /EC_number="1.8.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016876215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HIR3697303.1"
                     /translation="MVRTLSTMLDLGTEAPDFSLPDVVSGRTVSLDSFKDTKALLVMF
                     ICRHCPYVKHVQGELARIGRDYQDKDVAVVAISSNDAAEYPDDAPDSLKEQAEEQGFV
                     FPYLYDEDQSAAKAYTAACTPDFFLFDKERHLVYRGQLDDSRPKSDTPVTGRDLRAAL
                     DAVLSDAPVTEDQRASLGCNIKWKTGNEPEYFGAQ"
     gene            complement(2036..3241)
                     /locus_tag="AC159P_17565"
     CDS             complement(2036..3241)
                     /locus_tag="AC159P_17565"
                     /inference="COORDINATES: protein motif:HMM:NF017442.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF790 family protein"
                     /protein_id="HIR3697304.1"
                     /translation="MLTADLALSWQRGDRIRPRYLDTEDAKYLRVAEDFVRLFERHEG
                     ETRAELERSLTEYVGTGTDYKILRGLMKLLTDRCEFETAAGVEPAEARRALFLKARER
                     HPLVDEAARGETIAAVARELGADPAALLDGLYADLPDNQRLVSFETMAGHELLDLYNV
                     AQAQALLYRCVEMRLWVEPQDPEGYRELFGAIKAYRLIHTVRGNSREGYEISLDGPVS
                     IFQRSQKYGVQMAVFLPALLLCAGWRMRAEIEAKRRGTAFFELTSRQTQLRSHYLGAI
                     PYHNPVIEKLAEGWGRAESAWTLEKSSEVVAVGDSAFIPDFRIYSPAGDVVYLEILGF
                     WTPEHLRARVAEFEKAGLRNFIVAAWDELRGSRDPVTTVPPNTIIFKKNLDPALVELT
                     VERIVGSRQ"
     gene            complement(3342..4751)
                     /locus_tag="AC159P_17570"
     CDS             complement(3342..4751)
                     /locus_tag="AC159P_17570"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIR3697305.1"
                     /translation="MNATPLVLRFDSGTLLLEGAEASARVPSAFRWDERVLRWRAPAW
                     EYRSVVRELVRGGVAFEDAARAYHEFDFPTKLLVEPRPYQREAIEEWRRHGSRGVVVL
                     PTGAGKSLVAQMAIEQVKRSTLVVVPTIDLMNQWYDLLLSCFRAEVGLIGGGFFETGA
                     LVVSTYASAFRFMERLGNQFGLVIFDECHHLPSSVYRYAAELSIAPFRLGLSATPERA
                     DGAEAMLERLVGPFVFRRETHELAGEYLADYTVARVRVELSAEERAAYERERAIFRGF
                     LTDKGIGLGGLRGWQMFVAASARTPEGRRAMMAYRESKRIALGTDAKLRVLGELLQRH
                     RKERVLIFTAENEMVYRISRKFLIPAITHETNIKERRAWLEAFNEGRVLALTTSKVLN
                     EGVNIPEASVAVVLSGSGSTREHIQRLGRILRKVPDKEAVLYEVVTQDTTEEGISKRR
                     GSGDQFRAASRPEQGEWFA"
     gene            complement(4903..5805)
                     /locus_tag="AC159P_17575"
     CDS             complement(4903..5805)
                     /locus_tag="AC159P_17575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697306.1"
                     /translation="MGEEFSTEGAPAALERSQFMPALSIELAVERYNAVTEFVSRVLR
                     RDVDYGVIPGTEKRTLLKPGAEKLTTFFGLSTRFQLVERIEDWSGESFGGEPFFYYLY
                     RCRLHRGPVLMAEADGSCNSREQKYRWREAQRVCPECNQAAIIKGREEYGGGWLCFRK
                     KGGCGAKFGDGDAVIESQQTGRVFNAEIADQVNTMQKMAQKRSLVAAVLLAVNASEFF
                     TQDVEDMAIPTTAVVKDAGAPAAGAVEDEIRAACRGLGKTEEQLAAWVRKKYGAEGIA
                     QLTTEDKRDVLTFLQGMLTKREAA"
     gene            complement(5954..6412)
                     /locus_tag="AC159P_17580"
     CDS             complement(5954..6412)
                     /locus_tag="AC159P_17580"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HIR3697307.1"
                     /translation="MSFNKIIVVGNLGRDPELRYTPQGTAVCSFTMATNERRKGQAGE
                     PQDVTTWFRVTVWGKQAETVSKYLTKGRNVYVEGRLHVGEWSDKDGKQRYTLEVNATD
                     VRFIDGGQGVEGIPVRQAAQAAAQPSGRLAAGGTRPPAHDAGLEDDEIPF"
     gene            complement(6624..>7085)
                     /locus_tag="AC159P_17585"
     CDS             complement(6624..>7085)
                     /locus_tag="AC159P_17585"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015204831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042781 - 3'-tRNA processing
                     endoribonuclease activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HIR3697308.1"
                     /translation="REGIPAGPLYGRLKRGERVTLADGREFEGADFCGPTERGRTVVY
                     CTDTIYCESAVELARGADLLIHEATFAGDAEDLARRSLHSTAEMAARVAREAGVRKLY
                     ITHLSPRYVHAAPVTPEDLLREARAVFPDTELAHDFLSVEVPRHKEERVKG"
BASE COUNT         1202 a         2351 c         2254 g         1278 t
ORIGIN      
        1 agtaagcagt taagaactct cgactttcta ttttgcactt tcgaattctt atcctgcctt
       61 aactgcctac cgcctactgc ctactgcctg cttctttatg attaccatcg acggctcatt
      121 cggcgagggc ggcgggcaga tcatccgcac gtctctggcg ctgtcgctga ttacgggcaa
      181 agcgttccgc gtgtacaacg tgcgcgcggg gcgcgcgaag ccggggttgc agcggcagca
      241 cctgacggcg gtcacgtccg ccgcgaccat cggccacgcg aaggtggacg gcgcagggct
      301 aggggcgaag gagttcacgt tcgtgccggg cgaggtggtg ccgggcgagt acacgttcag
      361 catcggcacg gcggggagca cgatgctcgt gcttcaggcc gtcctgccgc cgctgatgat
      421 cgcgggcgcg ccctcgctgc tcgtgttcga gggcggcacg cacaacgtcc acgcgccgcc
      481 gttcgaattc atcaagaaga ccttcctccc gctcgtcaac cggacggggc cgaacgtctc
      541 ggtcgagctt cagcgctacg gcttctaccc gccgggcggc gggcgcttca acgtctacgt
      601 cgaacccgcc gccgcgctcc gtcggctcga cctcctggag cgcggccccg tcatcagtga
      661 gcgcgcccgc gcgctcgtcg tcaacatccc cgagcacatc ggcgagcgcg agctacaggt
      721 cgtcagccaa cagctcggct ggacgcagga tcagctcgaa ctcgaaacgt cgcgcaacgc
      781 cgtcagcggc ggcaacgtgc ttaccatcga gatcgagagc gagtacctga ccgaggtctt
      841 caccggcatc ggcgagcgcg gcgtgcgcgc cgaagtgatc gcgtcgcgcg ccgtccggga
      901 ggcgcagcgc tacctcgaca tcggcgcgcc ggtcggcgag cacctcgcgg atcagctcct
      961 gataccgatg gcgctcgcgg gcggcggtgc ctacacgacc ggcccgctca cgcctcacac
     1021 gacaaccaac atcgaggtca tcaggaagtt cctccccgtc gagatcgggc ggacgcagtt
     1081 gaccgaggac gtgtggaaag tcgaggtcga aagctgattc ggaacagaag tcagaagtca
     1141 gaacgatgaa cgatgaatga aaagcattct gtctttcatt catccttctg cgttctgact
     1201 tctggctttt gcataacgtc cgccatcggc taagatttcc gcacaaacat ctttcgcagc
     1261 cgtcaggagg aatcattatg gtcaggacgc tctccaccat gctcgatctc ggcacagagg
     1321 cgccggactt cagtctgccc gacgtggtct cgggccggac ggtctcgctc gattcgttca
     1381 aggacacgaa ggcgctgctc gtgatgttca tctgccgcca ttgcccctac gtcaagcacg
     1441 tgcaaggcga gctggcgcgc atcggcaggg actatcaaga caaggatgtc gccgtcgtgg
     1501 ccatcagctc gaacgacgcc gccgagtacc cggacgacgc gcccgatagt ttgaaggagc
     1561 aggccgagga gcagggcttc gtcttcccct acctctacga cgaagaccag tcggcggcga
     1621 aggcttacac cgcagcctgc acgcccgact tcttcctgtt cgacaaggag cgccacctcg
     1681 tctaccgcgg acagctcgac gacagccgcc ccaagagcga cacgcccgtc acgggccgcg
     1741 accttcgcgc cgcgctcgac gccgtgctct cggacgcgcc cgtcacggaa gaccagcgcg
     1801 ccagcctcgg ctgcaacatc aagtggaaga ccggcaacga gccggagtat ttcggcgcgc
     1861 agtagtaaac agtaggccgt aagcagtagg cggtaggcag tcaaagcaag aggcttgaga
     1921 tgaaatgtct caagcctctt cgttcgacag gcagtaaagc aaggaggctt gaggtggaac
     1981 gtctcaagcc tcttcgctct cctgtcttct gttctctatc ttgactgcct accgcctact
     2041 gcctacttcc cactattctt tcgaccgtca gctcgacgag cgcggggtcg aggttctttt
     2101 tgaagatgat ggtgttgggc gggacggtgg tgacggggtc gcggctgccg cgcagctcgt
     2161 cccacgcggc gacgatgaag ttgcgcaggc ccgctttctc gaactcggcg acgcgcgcgc
     2221 gcaggtgttc gggcgtccag aagccgagaa tttcgaggta gaccacgtcg cccgcgggac
     2281 tgtaaatacg gaagtcgggg atgaacgcgc tgtcgccgac ggccaccacc tcgctgctct
     2341 tctccagcgt ccacgcgctc tcggcgcgac cccagccttc ggcgagtttt tcgatgacgg
     2401 gattgtgata agggatcgcg ccgaggtagt gcgagcgaag ctgcgtctgc ctgctcgtca
     2461 gctcgaagaa ggcggtgccg cggcgtttgg cttcgatctc ggcgcgcatg cgccagcccg
     2521 cgcagagcag gagcgcgggg aggaagaccg ccatctgcac gccgtacttc tgcgagcgct
     2581 ggaagatcga gaccgggccg tcgaggctga tctcgtagcc ctcgcgcgag ttgccgcgca
     2641 ccgtgtggat gaggcggtac gccttgatcg cgccgaacag ctcgcggtag ccttccgggt
     2701 cttgcggctc gacccacaga cgcatctcga cgcagcggta gagcagcgcc tgcgcctgcg
     2761 cgacgttgta gaggtcgagc agctcgtggc ccgccatcgt ctcgaaggag acgagccgct
     2821 ggttgtccgg caggtctgcg tagaggccgt cgagcagcgc ggcggggtcg gcgccgagtt
     2881 cgcgcgcgac cgccgcgatt gtctcgccgc gcgccgcttc gtcaaccaac ggatgacgct
     2941 cgcgcgcctt gaggaagagc gcgcggcggg cctccgccgg ctcgacgccc gccgcggtct
     3001 cgaattcgca gcggtcggtc aaaagcttca tcagcccgcg caggattttg tagtccgtcc
     3061 ccgtcccgac gtattcggtc aaagaccgct caagctcggc gcgcgtctcg ccctcgtgcc
     3121 gctcgaagag gcgcacgaag tcctcggcca cccgtagata cttcgcgtct tcggtgtcca
     3181 gatagcgcgg cctgatgcgg tcgccgcgct gccagctcag tgcgagatcg gcggtgagca
     3241 taaaatgaaa atgatgaatg atgaatgatg aatgaaagac aagctgaagg atgagaagca
     3301 gcgtgcttct tattcatcat tcatcattcc gcgttcatcg ttcaggcgaa ccactcgccc
     3361 tgctcggggc gactcgcggc gcggaactgg tcgccgctgc cgcgccgttt actgatgccc
     3421 tcctcggtcg tgtcctgcgt gacgacttcg tagagcacgg cctccttgtc gggcaccttg
     3481 cggaggatgc gcccgagacg ctggatgtgc tcgcgcgtcg agccggagcc ggagaggacg
     3541 acggcgaccg acgcctcggg gatgttgacg ccctcgttca agaccttcga ggtggtcagc
     3601 gccagcacgc gcccctcgtt gaaggcttcg agccacgcgc gccgctcctt gatgttcgtc
     3661 tcgtgcgtga tggcggggat cagaaatttg cgcgagatgc ggtagaccat ctcgttctcg
     3721 gcggtgaaga tgaggacgcg ctctttgcgg tggcgctgca acagctcgcc gagcacgcgc
     3781 agtttggcgt ccgtgccgag cgcgatgcgc ttcgactcgc ggtaggccat catcgcgcgc
     3841 cgcccttccg gcgtgcgcgc gctcgccgcg acgaacatct gccacccgcg caggccgccg
     3901 agtccgatgc ctttatccgt caggaagccg cgaaagatgg cgcgctcgcg ctcgtaggcc
     3961 gcgcgctctt ccgccgagag ttcgacgcgg acgcgcgcca ccgtgtagtc ggccagatac
     4021 tcgcccgcca gctcgtgcgt ctcgcggcgg aagacgaagg ggccgacgag tctttcgagc
     4081 atcgcttctg ccccgtcggc gcgctcgggc gtcgccgaga gtccgaggcg gaacggcgcg
     4141 atggacagtt ccgcggcgta gcggtagacg ctcgacggaa gatgatgaca ctcgtcgaag
     4201 atgacgaggc cgaactggtt gcccagacgc tccatgaagc ggaaggccga ggcgtaggtc
     4261 gagacgacga gcgcgcccgt ctcgaagaag ccgccgccga tcaagcctac ctccgcgcgg
     4321 aagcacgaga gcagcaggtc gtaccactgg ttcatcaaat cgatcgtcgg caccacgacc
     4381 agcgtcgagc gcttcacctg ctcgatcgcc atctgcgcga cgaggctttt ccctgcgccc
     4441 gtcggcagca ccaccacgcc gcggctgccg tgccgccgcc actcctcgat cgcctcgcgc
     4501 tgataaggcc gcggctcgac cagcagcttc gtcggaaagt cgaactcgtg atacgcgcgc
     4561 gccgcgtcct cgaacgcgac cccgccgcgc accagctccc gcaccacgct ccggtactcc
     4621 caggccggag cgcgccaacg cagcacgcgc tcgtcccaac ggaacgccga cggcacgcgc
     4681 gccgacgcct ccgcgccttc gagcagcagc gtgccggagt cgaacctcaa cacaagcggc
     4741 gtcgcattca tcaagcgaga gcttacggaa gagtgaaggc aaaaggcaaa gtgcaaaagg
     4801 caaaggggcg gccacgcctg cggcgtgaga taacaaattg aaatcgaagc gaagcttcgt
     4861 ccacattttt gccttttgcc ttttgcctct tgccttcctc gcttacgcgg cctcgcgttt
     4921 cgtcaacatg ccttgcagga acgtcaacac gtcgcgcttg tcttcggtcg tcaactgcgc
     4981 gatgccctcc gcgccgtact tcttcctgac ccacgcggcg agctgctcct ccgtcttccc
     5041 taacccgcga caggcagcac gaatctcatc ctcgaccgcg cccgccgccg gtgcgcccgc
     5101 gtccttgacg acggcggtcg tcgggatggc catgtcttcc acgtcctgcg tgaagaactc
     5161 ggaggcgttg acggcgagca ggacggcggc gacgagcgat cttttttgcg ccatcttctg
     5221 catcgtgttg acctgatcgg cgatctccgc gttgaagacg cggcctgtct gctggctctc
     5281 gatgacggcg tcgccgtctc cgaacttggc gccgcacccg cccttctttc taaaacaaag
     5341 ccatcctccg ccgtattctt cgcggccctt gatgatggcc gcctggttgc attcggggca
     5401 cacgcgctgc gcctcgcgcc agcggtactt ctgttcgcgc gagttgcagg agccgtcggc
     5461 ttctgccatc aggacggggc cgcggtggag ccggcagcgg tagaggtagt agaagaaagg
     5521 ctcgccgccg aagctctccc ccgaccagtc ctcgatgcgc tcgacgagct gaaagcgcgt
     5581 cgagaggccg aagaaggtcg tcagcttctc cgcgcccggc ttcaggagcg tgcgcttctc
     5641 cgtgccgggg atgacgccgt agtccacgtc cctgcgcagc acgcggctga cgaactccgt
     5701 caccgcgtta taacgctcga ccgccagctc gatcgacagc gccggcatga actgcgaacg
     5761 ctccaacgcc gccggcgcgc cctccgtcga aaactcttcg cccatgtgtt cacccacctt
     5821 gctgcgattg cggaatgcgg atttcggatt gcggattgga agaagcgtgc ctcccgtcaa
     5881 ccgcatccgc gacgacggga gacagcggct ccttcatcga ccacgattcc gcattccgca
     5941 ttccgcgatt ccgctagaac gggatttcgt cgtcctccag ccccgcgtcg tgtgcgggcg
     6001 gtctcgtgcc tccggcggcg aggcggcccg agggctgcgc ggcggcctgt gccgcctgcc
     6061 gcacggggat gccttcgaca ccctgcccgc cgtcgatgaa gcgcacgtcc gtcgcgttga
     6121 cttccagcgt gtagcgctgc ttgccgtcct tgtccgacca ctcgccgacg tgcaggcggc
     6181 cctcgacgta gacgttgcgc cccttcgtca ggtacttcga gacggtctcg gcctgcttgc
     6241 cccagaccgt cacgcggaac caagtcgtca cgtcctgcgg ctcgcccgcc tgacctttgc
     6301 gccgctcgtt cgtcgccatc gtgaacgagc agaccgccgt gccctgcggc gtgtaccgca
     6361 actccgggtc gcggcccaga ttcccgacga cgatgatctt gttgaatgac atctctctcg
     6421 ctcctcgttt cattttttct cgcgggccgg catacgctcg cccgccgcgt ttcgggcgtg
     6481 gcacatcgta tcacacgtaa cagccctcgt gtaaagcaaa ggtgaaaagg tgaagagggg
     6541 aaagaggtaa aggggaaaag gcgaaaaggc gaagaggatg agaagcagcg cggttcgcgt
     6601 ctcccttttc accctttcgc cttttaccct tttacccttt cttccttgtg tctggggact
     6661 tcgacggaga ggaagtcgtg tgcgagttcg gtgtcgggga agacggcgcg cgcttcgcgc
     6721 aggaggtctt cgggggtgac gggcgcggcg tggacgtagc gcgggctgag gtgcgtgatg
     6781 tagagcttgc ggactccggc ctcgcgtgcg acgcgggcgg ccatctcggc ggtcgagtgg
     6841 agactgcggc gggcgaggtc ttccgcgtcg ccggcgaagg tcgcttcgtg gatgaggagg
     6901 tccgcgccgc gcgccagctc gacggcgctc tcgcagtaga tcgtgtcggt gcagtagacg
     6961 acggtgcgcc ctcgctcggt ggggccgcag aagtccgcgc cctcgaactc gcgcccgtcg
     7021 gcgagcgtca cgcgctcgcc gcgcttgagc ctgccgtaga gcggcccggc ggggatgccc
     7081 tctct
//
LOCUS       DBNGOH010000902         1695 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1969355, whole genome shotgun sequence.
ACCESSION   DBNGOH010000902 DBNGOH010000000
VERSION     DBNGOH010000902.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1695)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1695)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1695
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1969355"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1176
                     /locus_tag="AC159P_17590"
     CDS             <1..1176
                     /locus_tag="AC159P_17590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010073024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR3697309.1"
                     /translation="IKIFAARPDHVGALLCAAEASDALGDRQRAEGYRRIAGALAAPQ
                     DAKPEAGGAAEQGGELKSLDEGLEEVFGPGGGDREEKSGRKAAARTVADGETAEGYWE
                     AEPSDVKLSDVAGMDEVKRRLNLSLLAPLRNPEMMKLYGKSLRGGLMLYGPPGCGKTF
                     IARAVAGEMSARFIAVGLADVLDMYIGQSERNLREIFETARRNAPCVLFVDEIDALGR
                     KRSLQRHSGGRDVVNQLLSEMDSIGNDNRGVFVLAATNHPWDVDTALRRPGRFDRTLL
                     VLPPDPAAREAILRYHLGARPAEGVDLRWVAEKSEGYSGADLAHLVESAAELAMEESI
                     QSGKARPLRQNDFKRSMKEIRPSTRPWFEVARNYAMFSNEGGMYDELLDYLRSKRMV"
     gene            1303..>1695
                     /locus_tag="AC159P_17595"
     CDS             1303..>1695
                     /locus_tag="AC159P_17595"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3697310.1"
                     /translation="MNEEFVINNAGGHIERARVLMEQERHELAEGELRRHILASPDDA
                     TAHAMLALCLSGQMKHKAAIESAQRAVSLAPNFPYAHYALAWVSNNADRLAEAEHAID
                     EAIRLDPEEADYFAVLSSIKLQRRRWQEA"
BASE COUNT          298 a          550 c          597 g          250 t
ORIGIN      
        1 atcaagattt tcgcggcgcg gcccgaccac gtcggcgcgc tgctgtgcgc ggcggaggct
       61 tcggacgcgc tgggcgaccg gcagcgggcc gagggctacc gccgcatcgc gggtgcgctc
      121 gccgcgccgc aggacgcgaa gccggaggcc gggggcgcgg cggagcaagg gggcgaattg
      181 aagtcgctcg acgaagggtt ggaggaggtc ttcggccccg gcggcggcga ccgcgaagag
      241 aagtcgggcc gcaaggccgc ggcgcgcacg gtggccgacg gcgagaccgc ggaaggttac
      301 tgggaggcag agccttcgga cgtgaagctc tcggacgtgg cggggatgga cgaggtgaag
      361 cgccgcctca acctctctct gctcgccccc ttgcgcaacc ccgagatgat gaagctctac
      421 ggcaagagct tgcggggcgg cctgatgctc tacggcccgc cgggctgcgg caagaccttc
      481 atcgcgcgcg cggtggcggg cgagatgagc gcgcgcttca tcgccgtcgg gctggccgat
      541 gtgctcgaca tgtacatcgg ccagagcgaa aggaacctgc gcgaaatctt cgagaccgcg
      601 cgccgcaacg cgccgtgcgt gctcttcgtg gacgagatcg acgctctggg tcgcaagcgc
      661 agcctgcaaa gacattcggg cgggcgggat gtcgtcaacc aactgctctc ggagatggac
      721 agcatcggca acgacaaccg cggcgtcttc gtcctcgccg ccaccaacca cccgtgggac
      781 gtggacacgg cgctgcgccg tccgggcagg ttcgaccgca cgctgctcgt gctcccgccc
      841 gaccccgcgg cgcgcgaagc cattcttcgt tatcatctgg gggcgcgccc cgccgaaggc
      901 gttgacctgc ggtgggtcgc ggagaaatcg gaaggctact ccggcgcaga cctcgcgcac
      961 ctcgtcgagt cggcggcgga gctggcgatg gaggagtcca tccagagcgg caaggcccgc
     1021 cccctgcgcc agaacgactt caagcgctcg atgaaagaga tcaggcccag cacccgcccc
     1081 tggttcgaag tcgcgcgcaa ctacgccatg ttctccaacg aaggcggcat gtacgacgaa
     1141 ctgctcgact acctgaggtc gaagcggatg gtttaagagc cgtggaccgt aaaccgtgac
     1201 aagataaaac agggtctttc ttctcacgct tgacggctca cgctgcgcgg cctttctttt
     1261 cactcatcac ggttcacggt ttacggttca cggcttttat ctatgaacga agagttcgtc
     1321 atcaacaacg ccggggggca catcgagcgc gcgcgtgtgc tgatggagca ggagcggcac
     1381 gagctggcgg agggcgagct gcgccgccac atcctcgcgt cgccggacga cgcgacggcg
     1441 cacgcgatgc tggcgctgtg cctgtcgggg cagatgaagc acaaggcggc catcgagtcg
     1501 gcgcagcggg ccgtgagcct cgcgccgaac ttcccgtacg cgcactacgc gctggcgtgg
     1561 gtctccaaca acgccgaccg gctggccgag gcggagcatg ccatcgacga ggccatccgc
     1621 ctcgacccgg aagaggccga ctacttcgcc gtgctgagca gcatcaagtt gcagcgccgt
     1681 cgctggcagg aggcg
//
LOCUS       DBNGOH010000903         1629 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2520360, whole genome shotgun sequence.
ACCESSION   DBNGOH010000903 DBNGOH010000000
VERSION     DBNGOH010000903.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1629)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1629)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1629
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2520360"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1040)
                     /locus_tag="AC159P_17600"
     CDS             complement(<1..1040)
                     /locus_tag="AC159P_17600"
                     /EC_number="6.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015995 - chlorophyll biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase ATPase subunit I"
                     /protein_id="HIR3697311.1"
                     /translation="MALPKTLGELKRQGYRVRSVKDELRANLLGKLRAGEKIFPGIVG
                     YDETVVPQIVNAVLARHNLILLGLRGQAKSRIIRQLVQLLDERVPVIAGSEVNDDPFK
                     PISAYGRRLIEKEGDATPINWVGRDERFVEKLATPDVTVADIIGDVDPIKAARGGHLL
                     SDELTIHYGLLPRANRGLFAINELPDLAGKIQVGLFNIMQEGDVQIKGYPVRLALDVM
                     LVFSANPEDYTARGKIITPLKDRIGAEVMTHYPTDLQLGVEITRQEAWTLREGLDAAL
                     AIPDFIRELVEQIAFEARDDQRIDKRSGVSQRLPISVTESVVSNAERRALTTDETEIV
                     PRVSDVYAAVPS"
     gene            complement(1027..>1629)
                     /locus_tag="AC159P_17605"
     CDS             complement(1027..>1629)
                     /locus_tag="AC159P_17605"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3697312.1"
                     /translation="EPLAGKVRVDRFIEEGETIELGGDPPVVLRAMHTPGHARGHLSF
                     YEERTGALLTGDNIVGIGSVLIDPPEGDMRAYFATLERYRALPHLSVLFGGHGPAVGS
                     PRAKIDECVAHRQERERRILDAVREGARTPADIVARVYTDVHPKLHAMAERAVLAHLD
                     KLESDGAVARDGDLSGWRADILDPQNKASSGDNVSSDGTS"
BASE COUNT          238 a          492 c          596 g          303 t
ORIGIN      
        1 atcgagggga cggcagcgta cacgtcggag acgcgcggga cgatctccgt ttcgtcggtc
       61 gtgagcgcgc gccgctcggc gttcgagacg acggattcgg tgacggagat ggggaggcgc
      121 tgcgagacgc ccgagcgctt gtcgatgcgc tggtcgtcgc gggcctcgaa ggcgatctgc
      181 tcgacgagtt cgcggatgaa gtcggggatg gcaagcgcgg cgtccagccc ttcgcgcaga
      241 gtccacgcct cctgtcgcgt gatctcgacg ccgagctgca agtcggtcgg gtagtgcgtc
      301 atcacctctg cgccgatgcg gtctttgagc ggcgtgatga tcttgccgcg ggcggtgtag
      361 tcttcggggt tggccgagaa gacgagcatc acgtcgagcg cgaggcggac ggggtagccc
      421 ttaatctgca cgtcgccctc ctgcatgatg ttgaagaggc cgacctgtat tttgcccgcg
      481 aggtcgggca gctcgttgat ggcgaagagg ccgcggttgg cgcgcggcag caggccgtag
      541 tggatggtca gctcgtcgct caacaggtgg ccgccgcgcg cggccttgat cgggtcaacg
      601 tcgccgatga tgtcggccac agtcacgtcg ggcgtggcga gcttttcgac gaagcgctcg
      661 tcgcggccca cccagttgat gggcgtcgcg tcgccttcct tttcgatcaa ccggcgaccg
      721 taggcgctga tgggtttgaa cgggtcgtcg ttgacttcgg agccggcgat aacaggcacg
      781 cgctcgtcga ggagctggac gagctggcgg atgatgcggc tcttggcctg accgcgcaat
      841 cccaacaaaa tcaggttgtg ccgcgcgagg acggcgttga cgatctgcgg gacgaccgtc
      901 tcgtcgtagc cgacgatgcc ggggaaaatc ttttcgcccg cgcgcagctt gccgaggagg
      961 ttggcgcgca gctcgtcctt gaccgagcgc acgcggtagc cctgccgttt gagttcgccg
     1021 agtgttttag gaagtgccat cgcttgaaac attgtccccc gagcttgcct tgttctgtgg
     1081 gtcgagtata tcagctctcc acccactcaa gtcgccgtcg cgcgcgaccg ccccgtcgga
     1141 ttcgagcttg tcgaggtgcg ctagcacggc tcgttcggcc atcgcgtgca gcttcgggtg
     1201 cacgtccgtg tagacgcgcg cgacgatgtc cgcaggcgtg cgcgcgcctt cgcgcacggc
     1261 gtcgaggatg cggcgctcgc gctcctgtcg gtgcgcgacg cactcgtcga tcttggcgcg
     1321 cgggctgccg accgcggggc cgtggccgcc gaagagaacc gagaggtgcg gcaaggcccg
     1381 gtagcgttcg agcgtggcga agtaggcgcg catgtcgcct tcgggcgggt cgatgaggac
     1441 ggagccgatg ccgacgatgt tgtcgcccgt gaggagcgcg cccgtgcgct cttcgtaaaa
     1501 ggagaggtgg ccgcgcgcgt ggccgggcgt gtgcatcgcg cgcaggacga cgggcgggtc
     1561 gccgccgagt tcgatggtct cgccctcttc gataaaacgg tcaacgcgga ctttccccgc
     1621 gagcggttc
//
LOCUS       DBNGOH010000904         2018 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3069949, whole genome shotgun sequence.
ACCESSION   DBNGOH010000904 DBNGOH010000000
VERSION     DBNGOH010000904.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2018)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2018
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3069949"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            108..263
                     /locus_tag="AC159P_17610"
     CDS             108..263
                     /locus_tag="AC159P_17610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697313.1"
                     /translation="MADEREEKTCANPPCTCPVQGEKYCSVHCESTANTVQIDCDCGH
                     DTCKGDF"
     gene            422..979
                     /locus_tag="AC159P_17615"
     CDS             422..979
                     /locus_tag="AC159P_17615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HIR3697314.1"
                     /translation="MRTVALAVVLIWCVCLSAPPRARSAQTRTGTNAAAVVPCDIHAY
                     VVDKDPNGLNVRSGPAGTFDVVGNLPNQKVDGGIGVHITGSSGDWFRIDEAFEEGGEE
                     ERVFFKGVGWVYGPLLGSDGVGGGTNVYKEMSPKSRVVARVPGDSGGLTLRGCKGRWT
                     YVEYKKVKGWTAPGTLCANPLTTCP"
     gene            1028..1525
                     /locus_tag="AC159P_17620"
     CDS             1028..1525
                     /locus_tag="AC159P_17620"
                     /inference="COORDINATES: protein motif:HMM:NF025719.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsbRD N-terminal domain-containing protein"
                     /protein_id="HIR3697315.1"
                     /translation="MPSEMNALCDEMRGSIDALTLEWVDRVKSDHYIRSDNPLTLSQI
                     IDHVPQMLEELCDLLGQGGEPDFEAVRAASSHGYARALAGYSLTELLRELELLRECVF
                     DFVARAEAKRGVSRDETIRALRVVNQYFGEDTIFVVEHYLQRNARPQAAALDEAGGQE
                     EGRKG"
     gene            complement(1649..>2018)
                     /locus_tag="AC159P_17625"
     CDS             complement(1649..>2018)
                     /locus_tag="AC159P_17625"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3697316.1"
                     /translation="AVDDERDTLELVRVILERAGAVVTTATDAAAALDALRRTRPDVL
                     LTDIGMPVEDGYHLLRRVRALGADEGGQTPAIALTAYAGDADRSRTLAAGFQLHLPKP
                     VDAAQLVAVIANLAGTGERV"
BASE COUNT          341 a          681 c          668 g          328 t
ORIGIN      
        1 actgcctacc gcctactgcc taccgctctt tcccattgac tccgcgcccc tgtttggcat
       61 agaagctttc tacgcaataa atcaacttcg acggaggaga acctcagatg gccgacgaga
      121 gagaagagaa aacctgcgcg aacccgccgt gtacctgtcc cgttcagggc gagaagtatt
      181 gcagcgtcca ctgcgagagc accgcgaaca ccgtgcagat cgactgcgac tgcggtcacg
      241 acacctgcaa gggcgacttc taaacagggg ccgccagccg ccgccgcaaa cgctttttat
      301 tcggactcca aactcccggc cgggtcgaga gacgcttttc gcgccgagca cctgcggcga
      361 aatctttcgc cccgccggcc tcccttttcc aaccacccgc cagcggcttg aggaggacga
      421 gatgcgaacc gttgcgcttg ccgtcgtgct catctggtgc gtgtgcctgt ccgccccgcc
      481 acgcgcgcgg agcgcgcaga cccggacggg cacgaacgcc gccgccgtcg taccctgcga
      541 catccacgcc tacgtggtgg acaaagaccc caacggcctc aacgtgcgct ccggccccgc
      601 cggcaccttc gatgtcgtcg gcaacctgcc gaaccagaag gtggacggcg gcatcggcgt
      661 ccacatcact gggtcgagcg gcgactggtt ccgcatcgac gaagccttcg aggagggcgg
      721 cgaggaggag cgcgtcttct tcaagggcgt gggctgggtc tacgggccgc tgctcggctc
      781 cgacggggtg ggcggcggga cgaacgtgta caaggagatg tctccgaaga gccgcgtcgt
      841 ggcccgcgtg ccgggcgaca gcggcggcct caccctgcgc ggctgcaagg gccgctggac
      901 ttacgtcgag tataaaaagg tcaaaggctg gaccgccccc ggcacgctct gcgccaaccc
      961 gctgacgacc tgcccctgag ccgggtctgc ggaagaacag gccgacgacg aggataacga
     1021 aacccgtatg ccttccgaga tgaacgcgct gtgcgacgag atgcggggtt cgatcgacgc
     1081 cctcacgctg gaatgggttg accgcgtcaa gtccgaccat tacatccgct cggacaaccc
     1141 gctgacgctc tcgcagatca tcgaccacgt cccgcagatg ctcgaagagc tgtgcgacct
     1201 gctcgggcag ggcggcgagc ctgacttcga ggccgtccgc gcggctagct cgcacggcta
     1261 cgcgcgcgcg ctcgcgggct actcgctgac cgagctgctg cgcgagcttg agctgctgcg
     1321 cgagtgcgtc ttcgacttcg tcgcacgggc cgaggcgaag cgcggcgtca gccgcgacga
     1381 gacgatccgc gcgctccgcg tcgtcaatca atatttcggc gaggacacca tcttcgtcgt
     1441 cgagcactac ctccaacgca acgcgcgccc gcaggccgcg gcgctcgacg aagcgggcgg
     1501 gcaggaagaa gggcgaaaag gttaaaaggg gaagagggga aaaggaagac aagcgacgcg
     1561 cttcttcctt ttcccctctt ccccttttcc cccttcgcct ctcttccctt ttcgcctctt
     1621 ccccttttaa ccttttcgcc tctcttgttc acactcgctc gcccgtgccc gcgaggttgg
     1681 cgatgacggc gacgagttgc gccgcgtcca cgggtttggg gaggtggagt tggaagcccg
     1741 cggcgagggt gcgcgagcgg tcggcgtcgc ccgcgtaggc cgtgagcgcg atggcggggg
     1801 tctggccgcc ctcgtcggcg ccgagcgcgc gcacgcggcg gagcaggtgg tagccgtctt
     1861 cgacgggcat gccgatgtcg gtgagcagca cgtcggggcg cgtgcggcgg agcgcgtcga
     1921 gcgcggcggc ggcgtccgtc gcggtcgtca cgacggcgcc cgcgcgttcg aggatgacgc
     1981 ggacgagttc gagcgtgtcg cgctcgtcgt caaccgcg
//
LOCUS       DBNGOH010000905         3307 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2885976, whole genome shotgun sequence.
ACCESSION   DBNGOH010000905 DBNGOH010000000
VERSION     DBNGOH010000905.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3307)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3307)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3307
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2885976"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..975
                     /locus_tag="AC159P_17630"
     CDS             <1..975
                     /locus_tag="AC159P_17630"
                     /EC_number="1.17.98.2"
                     /inference="COORDINATES: protein motif:HMM:NF015983.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HIR3697317.1"
                     /translation="DLTFEEIARVSQTMPPITDLWLSGGEPTLRHDASEIIDLFVENN
                     GVRRVIIPTNGLVKTRAFEIVERDLSTHPELHLYLNVALDGYGETHDRVRGVPGHWRK
                     TLDCIEHLYPLKERFAHRFRLNVNTVVCAENYAEVERLAAFMWDNFRLDGHYFNIIRG
                     ETKAGAAIKQVPAEVLPAMYAKISALTKRYGDRMFAADDAATRFVKNAAYVGAITTHY
                     RTQHSNFERQTAWPFPCTAGETAAVIDYNGDVRACELRARFASLRDFDFDFGALWAAE
                     EHRRELASIDGGRACWCTHVCFIHDSMRHSRRALLWEVPKNHLTRERW"
     gene            1066..1326
                     /locus_tag="AC159P_17635"
     CDS             1066..1326
                     /locus_tag="AC159P_17635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697318.1"
                     /translation="MNIFKLLVALVVLLLAAFGAFALFGLLFATLKYVLVLGLVVLAA
                     GLGYRALTKKSDDPLVGAGWPERELEKAQRLLEGIKRGELTK"
     gene            1451..2107
                     /locus_tag="AC159P_17640"
     CDS             1451..2107
                     /locus_tag="AC159P_17640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697319.1"
                     /translation="MKILRSLALVLAFASVAAGQVYKEEAVKEESSRQVADMPASYFG
                     PFRVNGDKPRGFEGFDFFILGYKEAKDADKDERDVLVPDKQGNVTVRGELVTAKGTPL
                     AFETVKLTETGPVTFYYKGPTLSRITRAQPIRLSFTTVEVKGVKYAFSGEYLDEPDEE
                     AGGFTYLRGTFTKYKNGKPVAEAKTNFRRLAYQELTQEREPPPASAEEPPSGDASGQP
                     "
     gene            2150..2377
                     /locus_tag="AC159P_17645"
     CDS             2150..2377
                     /locus_tag="AC159P_17645"
                     /inference="COORDINATES: protein motif:HMM:NF021073.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HIR3697320.1"
                     /translation="MADEKNEQEENREVTRRSGIAYAAGLTIFLSVVSFMGVGWLLDR
                     WLGTGWIMIAGIVLGSVVGFYEFIRIITRIK"
     gene            2468..2908
                     /locus_tag="AC159P_17650"
     CDS             2468..2908
                     /locus_tag="AC159P_17650"
                     /inference="COORDINATES: protein motif:HMM:NF015834.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HIR3697321.1"
                     /translation="MSEMVDDAGASPTAGAGEDPSAMERRIFRGMCRAVALAVLISAP
                     FAPWRVTTGLLLGGVLALLNHHWLRTSVAAVFGAAPAGARPKMKAARYVLRYVVIAVV
                     VAAAYRLDLVSLAATLAGLCAFVAAALVEGIRQLYSAIAHREEA"
     gene            2913..>3307
                     /locus_tag="AC159P_17655"
     CDS             2913..>3307
                     /locus_tag="AC159P_17655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697322.1"
                     /translation="MFLLAESGGGGHHTPTIVEMINHYLGEPVHHFQVNNTKPLWDKF
                     LGYFGSSAEKAFGPYTPENAIPWYTIMFVLACILTMVVIWILKPRKLSEDEPGYGQLT
                     LETGVLAIRDLLSDNVGPKGMKYLPVIGT"
BASE COUNT          578 a         1123 c         1069 g          537 t
ORIGIN      
        1 gatttgacct tcgaagagat cgcgcgcgtc tcgcaaacga tgccgcccat caccgacctc
       61 tggctctcgg gcggcgagcc gacgctccgc cacgacgcct cggaaatcat cgacctcttc
      121 gtcgagaaca acggcgtccg ccgcgtcatc atcccgacca acggcctcgt caagacgcgc
      181 gccttcgaga tcgtcgagcg cgacctgtca actcatccgg agcttcacct ctacctcaac
      241 gtcgcgctcg acggctacgg cgagacgcac gaccgcgtgc gcggcgtgcc cggccactgg
      301 cgaaagacgc tcgactgcat cgagcacctc taccccttga aggagcgctt cgcgcaccgc
      361 ttccgcctca acgtcaacac cgtggtctgc gccgagaact acgcggaggt cgagcggctc
      421 gccgccttca tgtgggataa cttccgcctc gacggccact acttcaacat catccgcggc
      481 gagacgaagg cgggcgccgc catcaagcag gtgccggcgg aagtgctgcc cgcgatgtac
      541 gcgaaaatct ccgcgctcac caagcgctac ggcgaccgca tgttcgccgc ggacgacgcg
      601 gccacgcgct tcgtcaagaa cgcggcctac gtcggcgcca tcacgacgca ctaccgcaca
      661 cagcactcga acttcgagcg gcagaccgcg tggcccttcc cctgcacggc gggcgagacc
      721 gccgcggtca tcgactacaa cggcgacgtg cgcgcctgcg agttgcgcgc gcggttcgcc
      781 tcgctccgcg acttcgactt cgacttcggc gcgctgtggg ccgccgaaga gcaccgccgg
      841 gaactcgcat caatcgacgg cgggcgcgcc tgctggtgca cgcacgtctg cttcatccac
      901 gactcgatgc gccactcgcg gcgcgcgctg ctgtgggagg tgccgaagaa tcacctcacg
      961 cgcgagcgct ggtgagcgaa agtccgaacg ggccgcccgc gcgcgacgtt taaacgtgca
     1021 ggctgctccc cggcatcgga attctttcgc acggaggttt cggtcgtgaa catcttcaag
     1081 ctcctcgtcg cgctcgtcgt cctgctgctc gccgccttcg gcgcgttcgc gctcttcggc
     1141 ctgctcttcg cgacgctcaa gtacgtcctc gtcctcggcc tcgtcgtcct cgccgccgga
     1201 ctcggctaca gggcgctcac gaaaaagtcg gacgacccgc tcgtcggcgc gggctggccc
     1261 gagagggagc tggaaaaagc gcagcgcctg ctcgaaggaa tcaagcgcgg cgagttgacg
     1321 aaatagcgcg cgcgtcgcaa actcttctcc cgcgtgaccg caggcggcgg gaacggcggc
     1381 gccgaccgcc gccgccgccg aatcgacttt caccccgcgc gccgcgggag ttcgtttgcc
     1441 cgtcccgttt atgaaaattc ttcgatcgct ggcgctggtt ctcgcgttcg cctcggtggc
     1501 cgcggggcaa gtctacaaag aagaggcggt gaaggaggag tcgagccggc aggtcgccga
     1561 tatgccggcc tcgtatttcg ggccgttccg cgtcaacggc gacaagccgc gcggcttcga
     1621 gggcttcgac ttcttcatcc tcggctacaa ggaagcgaag gacgcggaca aagacgaacg
     1681 cgacgtgctc gtgccggaca agcagggcaa cgtcacggtg cgcggcgagc tggtcacggc
     1741 gaagggcacc ccgctcgcct tcgagacggt gaagctcacg gagaccgggc cggtgacctt
     1801 ttactacaag gggccgacgc tcagccgcat cacgcgcgcg cagccgatca ggctctcgtt
     1861 caccaccgtc gaggtcaagg gcgtgaagta cgccttcagc ggcgagtacc tcgacgagcc
     1921 ggacgaggag gcgggcgggt tcacgtacct gcgcggcacg ttcacgaagt acaagaacgg
     1981 caagcccgtc gccgaggcca agaccaactt ccggcggctg gcctaccagg aactgacgca
     2041 ggagcgggag ccgccgccgg catccgccga agagcctccg agcggggacg cctccgggca
     2101 gccttgagcc ttcacacgcg gatcgaactt cccgcagggc ggtgagccgt tggccgacga
     2161 gaagaacgaa caggaagaga accgagaggt cacgcgccgg agcggcatcg cctacgccgc
     2221 ggggctgacg atcttcctct ccgtggtgtc gttcatgggg gtgggctggc tgctcgaccg
     2281 ctggctcgga accggctgga tcatgatcgc cggcatcgtc ctcggctccg tcgtcggctt
     2341 ctacgaattc atccgcatca tcactcgcat caagtaagag taattcgggc gcgaaagaat
     2401 cccgccgccg gtcgcgtgca aactcgacag cccgcgcgaa agtccgctaa aattaatcgt
     2461 tccgaagatg agcgagatgg ttgacgatgc gggcgcctct ccgaccgccg gggcgggcga
     2521 agacccttcg gcgatggagc ggcgcatctt ccgcggcatg tgccgggcgg tcgcgctggc
     2581 ggtcttgatc agcgcgccct tcgcgccgtg gcgggtgacg acgggcctct tgctgggcgg
     2641 cgtgctcgcg ctcttgaacc accactggct gcggacttcg gtggcggctg tcttcggcgc
     2701 ggcgcccgcg ggcgcgaggc cgaagatgaa ggcggcgcgc tacgtgctcc gctacgtcgt
     2761 catcgccgtt gtggtcgccg ccgcctaccg gctcgatctg gtctcgctcg ccgccacgct
     2821 cgcggggctt tgcgcgttcg tcgcggcggc actcgtcgaa gggatcaggc agttatacag
     2881 cgccatcgcg caccgagagg aagcttaaaa agatgttcct gttggcagag tcagggggcg
     2941 gcgggcatca caccccgacc atcgtcgaga tgatcaacca ctatctcggc gaacccgtcc
     3001 accactttca ggtgaacaac acgaagccgc tgtgggacaa gttcctcggc tacttcggca
     3061 gcagcgccga gaaggcgttc ggcccctaca cgccggagaa cgccatcccg tggtacacga
     3121 tcatgttcgt gctggcgtgc atcctgacga tggtcgtgat ctggattctg aagccgcgca
     3181 agctctcgga ggacgagccg ggctacgggc agttgacgct tgagacgggc gtgctggcga
     3241 tacgcgacct gctgtccgac aacgtcgggc cgaaggggat gaaatacctg cccgtcatcg
     3301 gcacctt
//
LOCUS       DBNGOH010000906         2197 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_5086479, whole genome shotgun sequence.
ACCESSION   DBNGOH010000906 DBNGOH010000000
VERSION     DBNGOH010000906.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2197)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2197)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2197
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_5086479"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1001)
                     /gene="ligA"
                     /locus_tag="AC159P_17660"
     CDS             complement(<1..1001)
                     /gene="ligA"
                     /locus_tag="AC159P_17660"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HIR3697323.1"
                     /translation="MPASTKSIESEIRRLREEVLRHEELYYVRDDPEISDAEYDALVR
                     RLQTLEDEHPELRTPDSPTLRVGGRPAEGFDEYVHRRPMLSLDNSYNLEDLRAFDERC
                     RKLADGRAFEYVAELKIDGLSVSLHYENGVLVRGVTRGDGRRGEDVTPNVRTIRSVPL
                     RLKLADGGEVEVRGEAYLSRKTFERINAEREGSDEARFANPRNAAAGTIRQLDPKIVA
                     RRRLDLFAYDALRGDRKAFRTHWESLEWLVAAGFSVNEHRRKCEDIEAVIRFCNEMEA
                     ERDQLEYEIDGVVVKVNSTALQDEFGATAKAPRWAIAYKYPARQATTQVESIVVQVGR
                     "
     gene            complement(1100..2131)
                     /locus_tag="AC159P_17665"
     CDS             complement(1100..2131)
                     /locus_tag="AC159P_17665"
                     /inference="COORDINATES: protein motif:HMM:NF013003.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HIR3697324.1"
                     /translation="MRPDLSGASLSRRLDPAALRGIDLRGAGLAEVSLRGADLREANL
                     SGALLKDAHLNGADLSGADLSGALLITAVLSEAKLVGANLSGAVLGGAFFRDTDLSGA
                     NLSGANLKMARLVDSNLEGADLSDTLVYGISAWNLRLQGARQRNIVITSVGEPRITVD
                     NLEVAQFVYLLLNNEKIRDVINTVTSKAVLILGRFSPPERKSVLDAMRRKLRELNFLP
                     IVFDFERPTDRDFTETIMTLAGMSMFVIADITNPKSSPLELQSAVPNYQIPFVPIQQA
                     DEKPFAMFVNLQTKFDWVLKVRKYKTQDELIQFFERGVVRPALECHNRLMAAKTAAMG
                     EVDISSFAE"
BASE COUNT          328 a          703 c          763 g          403 t
ORIGIN      
        1 cggcccacct gcacgacgat ggactcgacc tgcgtggtcg cctgccgcgc cgggtacttg
       61 taggcgatgg cccagcgcgg cgccttcgcc gtcgcgccga actcgtcctg caaggccgtc
      121 gagttgacct tcacgacgac gccgtcgatc tcgtattcga gttggtcacg ctcggcctcc
      181 atctcgttgc agaaacggat gacggcctcg atgtcttcac actttcggcg gtgctcgttg
      241 acgctgaagc ctgcggcgac gagccattcg agcgattccc agtgcgtgcg gaaagccttg
      301 cggtcgccgc gcagcgcgtc gtaggcgaac aggtccaatc tcctgcgggc gacgatcttc
      361 gggtcgagct ggcggatggt tccggcggcg gcgttgcgcg ggttggcgaa gcgggcttcg
      421 tccgacccct cgcgctcggc gttgatgcgc tcgaaagtct ttctcgacag gtacgcctcg
      481 ccgcgcacct cgacctcgcc gccgtcggcg agcttgagac gtaaagggac ggagcggatg
      541 gtgcgcacgt tcggcgtcac gtcctcgccg cgccgcccgt cgccgcgcgt cacgccgcgc
      601 accaggacgc cgttctcgta gtggagcgag acggagaggc catcgatctt cagctcggcg
      661 acgtactcga aggcgcgacc gtcggcgagc ttgcggcagc gctcgtcgaa ggcgcgcagg
      721 tcttcgaggt tgtagctgtt gtcgagcgag agcatcgggc ggcggtggac gtactcgtcg
      781 aagccctcgg cgggccgacc gccgacgcgc agcgtggggc tgtccggcgt ccgcagctcc
      841 gggtgctcgt cctccaacgt ttgcaggcgc cggacgagcg cgtcgtactc cgcgtcggaa
      901 atctccgggt cgtcgcggac gtagtacagc tcttcgtggc ggaggacttc ctctcgcagc
      961 cggcggattt cgctctcgat gcttttcgtg ctggcaggca aggctcggtt caccaatcgg
     1021 gggcgcacag gatgtcggaa gacttccctt tcatcatagc ggaagcgtcc ggcgcgcgac
     1081 aacgcgagcg gcccccggct cattccgcga acgaagagat gtccacctcg cccatcgccg
     1141 ccgtcttcgc ggccatcagg cggttgtggc attcgagcgc gggccggacg acgccccgct
     1201 cgaagaactg tatgagttcg tcttgcgtct tgtacttccg caccttcagc acccagtcga
     1261 acttcgtctg caagttcacg aacatggcga acggtttctc gtcggcctgc tggatgggga
     1321 cgaaaggaat ctggtagttg gggacggcgg actgtagttc gagcgggctg gacttcgggt
     1381 tggtgatgtc ggcgatgacg aacatggaca tcccggcgag cgtcatgatg gtctcggtga
     1441 agtcgcggtc ggtcgggcgc tcgaagtcga agacgatggg gaggaagttc agctcgcgca
     1501 gcttgcgccg catcgcgtcg agcacgcttt tgcgctcggg cggcgagaag cgaccgagga
     1561 tgagcaccgc cttcgaggtg acggtgttga tcacgtcgcg gatcttctcg ttgttcagga
     1621 gcaggtagac gaactgcgcg acctccaggt tatcgacggt gatgcggggt tcgccgacgg
     1681 aggtgatgac gatgttgcgc tgccgcgcgc cctggagccg taggttccag gccgagatgc
     1741 cgtagacgag cgtgtcggag aggtccgcgc cttcgaggtt cgagtccacg agccgggcca
     1801 tcttcaggtt cgccccgctc aggttcgccc cgctcaggtc ggtgtcgcgg aagaaggcgc
     1861 cgccgaggac ggcgccgctc aggttcgcgc cgaccagctt cgcctcgctg agcacggcgg
     1921 tgatgagcaa agcgccgctc aagtcggccc cgctcaggtc ggcgccgttc aggtgcgcgt
     1981 ccttgagcag cgcgccgctc aggttcgctt cacgcaggtc ggcgccgcgc aggctcactt
     2041 cggccaggcc ggccccgcgt aagtcgatcc cgcgcagcgc tgcggggtcg agtcggcggc
     2101 tcagcgacgc gccgctgagg tcgggccgca cgccggggct gtccgctctc cacctgttcc
     2161 acgcctcgac gcccgattcg aaaatagcaa ggtgtta
//
LOCUS       DBNGOH010000907         2601 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3618940, whole genome shotgun sequence.
ACCESSION   DBNGOH010000907 DBNGOH010000000
VERSION     DBNGOH010000907.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2601)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2601)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2601
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3618940"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            635..1108
                     /locus_tag="AC159P_17670"
     CDS             635..1108
                     /locus_tag="AC159P_17670"
                     /inference="COORDINATES: protein motif:HMM:NF046562.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hfq-like protein"
                     /protein_id="HIR3697325.1"
                     /translation="MNRKLIRTTLAEVKGRGDSAPHRERPEGAGVRTGPPHRSGGGPP
                     SREGGDRGPREGGPSIPQGRKKVPPAETNAEIFYYKKQMDTHTQMVIVLVDGDEVEGT
                     IEWYDRNALKINRRGAPNIMLLKHNIKYMFKAEDRDGGGGDGGGGDDDDDAGDEG"
     gene            1221..2297
                     /gene="pdxA"
                     /locus_tag="AC159P_17675"
     CDS             1221..2297
                     /gene="pdxA"
                     /locus_tag="AC159P_17675"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HIR3697326.1"
                     /translation="MSEELPENTVAAAARGAERTLPRIGVTMGDPAGIGPEVVLKAVL
                     EPEVLAACVPFIVGDAQLLAHQARKLDLSCGYEIVRRGEEFPAEAGGPFIYHLDNVRS
                     DIEPGVESGEAGRAAAEYIEAAVELCAAGVVDAISTAPINKRALFLGGYSFPGHTEFL
                     AHLTRSEECAMAFVASNLRIVLISTHVPLAEAIRLVEKERIVRVVRLADRELRRWGLV
                     RPRIAVAALNPHGAEGGLFGVEESAEIIPAVEACRAGGLTEVSGPYSADTVFLRAARG
                     EFDAVISCYHDQAMIPVKCLSFGEAVNVTMGLPFIRTSVDHGTAFDIAGRGLAEHSSM
                     VAAITLAARLARADESNELVMNAE"
     gene            2417..>2601
                     /locus_tag="AC159P_17680"
     CDS             2417..>2601
                     /locus_tag="AC159P_17680"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR3697327.1"
                     /translation="MSSRKILVADDDPAILRLVSAIVEKEGYTVVAARDGREAYKHLQ
                     ESDFVACVFDVMMPHIQG"
BASE COUNT          453 a          860 c          863 g          425 t
ORIGIN      
        1 ttcgacaagg acatgtcgga agtgggcacg accagctcgg cgcacgtctc cgcgccgcgc
       61 tacacgccgt ccacagacct tcctccgccg cgcccgtcgg ggccgccgcg ctcggacgac
      121 ttggacgtgc cgaccttcat ccgcaagaag gcggactgaa ggaacagtag gcagtaggca
      181 gcgggcagca ggccgtcaag acggaaggac ggagaggctc gggactttac ctcccgagcc
      241 tcttcgttcg tcaagtagta agggaagaag cttgagaggt aaagtcccga gcctctccgt
      301 ccttgctaac cgctgctcgc tgcctgctgt ctgttgcttt tttcttctta actgcctgct
      361 gcctactgcc tgctgcccac ttttcccctc cgcgccttga actttgcatg ttcgtcgtgt
      421 agcatgtcgc gcatccctga caggattgaa attcaacaac gggtaaagac cgtttctcgt
      481 ctgtttactt ttcagaccgc agaccgtcgg caggggacgc gataggccgt cgccgtcacc
      541 tgtccgtccg gcagcccggt cgcgcatccg aagaccgggc cgaaccgcta cgcaatctat
      601 ccggctacgc tttgatggag gtgaggaggc taaggtgaac cgcaaactga ttcgaaccac
      661 gctggcagag gtcaagggcc gcggcgacag tgcgccgcac agggagcgac ccgagggcgc
      721 aggggtgcgc acggggccgc cgcaccgctc gggcggcggg ccgccgtcgc gcgagggcgg
      781 cgaccgaggg ccgcgcgagg gcgggccgtc catcccgcag gggcgcaaga aggttccgcc
      841 cgccgagacg aacgcggaaa tcttctacta caagaagcag atggacacgc acacgcagat
      901 ggtcatcgtg ctcgtggacg gcgacgaggt cgagggcacc atcgagtggt acgaccgcaa
      961 cgcgctgaag atcaaccgcc gcggcgcgcc gaacatcatg ctcttgaagc acaacatcaa
     1021 gtacatgttc aaggccgaag accgcgacgg cggcggcggc gacggcggcg gcggtgacga
     1081 cgacgacgac gcgggagacg agggctgacg caagggtcag gccctcgccg caccgttcgc
     1141 ggcgtccggt gtcgggccga gacggcggca gcagtcaacc tccctcatca tctccccctc
     1201 acagttcacg gcttcgagcg atgagcgaag agctacctga aaacacggtc gccgccgcgg
     1261 cgcgcggcgc cgagcgcacg ctgccgcgca tcggcgtcac gatgggcgac cccgcgggca
     1321 tcggccccga ggtcgtcctg aaggccgtcc ttgagccgga ggtgctcgcc gcgtgcgtgc
     1381 ccttcatcgt cggcgacgcg caactcttgg cgcatcaggc gcgcaagctc gacctcagtt
     1441 gcggctatga gatcgtgcgg cgcggcgagg agttcccggc ggaggcgggc ggccccttca
     1501 tctatcacct cgacaacgtc cgctcggaca tcgagccggg cgtcgagtcg ggcgaggcgg
     1561 gccgcgccgc cgccgagtac atcgaggcgg cggtcgaact gtgcgccgcc ggcgtcgtgg
     1621 acgccatctc gaccgcgccg atcaacaagc gcgcgctctt cctcggcggc tacagcttcc
     1681 ccggccacac cgaattcctc gcgcacctga cccgctcgga agagtgcgcg atggccttcg
     1741 tcgcgtcgaa cctgcgcatc gtcctcatct cgacgcacgt ccccttagcc gaggccattc
     1801 gcctcgtcga gaaggagcgc atcgtgcgcg tggtgcggct cgccgacagg gaactcaggc
     1861 ggtggggcct cgtcagaccg cgcatcgcgg tcgcggcgct gaacccgcac ggggccgagg
     1921 gcggtttgtt cggcgtcgag gagtcggcgg agatcatccc ggcggtcgag gcgtgccgcg
     1981 cgggcggtct gacggaggtg tcggggccgt actcggcgga cacggtcttc ctgcgggccg
     2041 cgcgcggcga gttcgacgcc gtcatttcgt gctaccatga ccaggcgatg atccccgtca
     2101 agtgcctctc gttcggcgag gccgtcaacg tcacgatggg gctgcccttc atccgcactt
     2161 ccgtagacca cggcaccgcc ttcgacatcg cgggccgcgg cctcgcggag cattcgagca
     2221 tggtcgccgc catcaccctc gccgcacgac tggcgcgcgc cgacgagagc aacgaattag
     2281 tgatgaatgc agaatgatga atgatgaatg aaagacaggt cggatgatga gaggcaccgt
     2341 gcttctcatt catcattctg cactctgcat tcatcgtttc atcacccgga ggtttccgcc
     2401 agtggccgag cagcctttgt cctcacgcaa aatcctcgtc gcggacgacg acccggcgat
     2461 cctgcgtctg gtctcggcca tcgtggagaa ggaaggctac acggtggtcg cggcgcgcga
     2521 cgggcgcgag gcgtacaagc accttcagga gtcggacttc gtggcgtgcg tcttcgacgt
     2581 gatgatgccg cacattcagg g
//
LOCUS       DBNGOH010000908         4736 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1788449, whole genome shotgun sequence.
ACCESSION   DBNGOH010000908 DBNGOH010000000
VERSION     DBNGOH010000908.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4736)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4736)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4736
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1788449"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1976)
                     /locus_tag="AC159P_17685"
     CDS             complement(<1..1976)
                     /locus_tag="AC159P_17685"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020661246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HIR3697328.1"
                     /translation="MLAVGITALAAGSTLFPGGARRAEAAQDAAAADKGISASAAAQI
                     GALAAEKRSRTPAQRKIDSQLLYASKMSRGEPIAAGVQSLSVSVGETERGRVVVDISG
                     TVGDALLQTLEANGARVLYASPAYGSLRAEVALEQLETIAASPDVRYIQPKQEAMTSR
                     RERANAGLDVPIGAGNPASALRPDFAARAARVREKLSSALAAYRTDDSPADGGVGVNS
                     VGARRAEGDVTHRADVARRRYNVDGTGVKIGVLSDGVVNLSFAQASGDLGEVTVLPGQ
                     AGSGDEGTAMLEIVHDLAPGAQLFFATAFGGITSFADNIRTLRDDYDCDIIVDDVFYF
                     AEAVFQDGQAPSIISNTNGGVVTQAVNDVTADGAEYFSSAGNEGNLNDGTSGVFEGNF
                     VNGGTIPVIGGAGAGPVHDFDPTAAVSQFNRITLGSGNPITLTWADPLGASNNDYDLY
                     ILNAAGTALVAASTGSQDGTQDPFELVGGGANVTNNRVVVAKFSGADRFLHTNTFRGR
                     YLFATAGQTHGHASAALAYAVAATPAVGPFPMPFNSTNMVETFESDGPRKLYFQPNGT
                     PYVPGDFLATGGITRQKPDITAADGGVVSGAGGFPNPFFGTSAAAPHAAAIAALVKDG
                     VPSITQAQMRAALNGSAIDIEAPGVDRDSGVGII"
     gene            complement(2257..2769)
                     /locus_tag="AC159P_17690"
     CDS             complement(2257..2769)
                     /locus_tag="AC159P_17690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697329.1"
                     /translation="MKRCEKLPLWSLVALAVVSAATPLAAAAQTTPPAPPQSQDQKVS
                     PEAARQIADLAREKRARTAAQKKIDTQLLYALKQKRGERRGVPSEPVDIKLDAHGRAL
                     VDITARVSAKLISKLGKLGAEVVSQSAKYHTVRVKVTLEKLEALAAIEEVRYISPAAQ
                     AMTHGGARTN"
     gene            complement(3125..3880)
                     /locus_tag="AC159P_17695"
     CDS             complement(3125..3880)
                     /locus_tag="AC159P_17695"
                     /inference="COORDINATES: protein motif:HMM:NF018389.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HIR3697330.1"
                     /translation="MVNIFGGAGSERMLFVARKMTKTTARSLVLLPAALFAVACAPQE
                     NAGRGNAKEAAAAKAETTTAGKPSPTPPTAARASLPPTEGKLTPADRKAWREIIRWPE
                     ECEEAFAGTAGRAGDNAGLEFFELGEGRRLLKVLCAGGAYQPSQVFAVVNEAGATPSA
                     KVLTFDVHESPEDGKLEKTSATELWGLADFDARTKQLKVHNRFRGPGDCGTLSTYSFD
                     DAGPVLKELRADTLCDGKGAENPEQWKKVTPAP"
     gene            complement(4026..4232)
                     /locus_tag="AC159P_17700"
     CDS             complement(4026..4232)
                     /locus_tag="AC159P_17700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697331.1"
                     /translation="MRDVKDLLRVVGIILLGMFVLAVVVPLVLTAAGIVVGTVVKLAV
                     LLIKLAVFLAIGYLVLVGVRAVLR"
     gene            4514..>4736
                     /locus_tag="AC159P_17705"
     CDS             4514..>4736
                     /locus_tag="AC159P_17705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HIR3697332.1"
                     /translation="MARPRRTTDKLFDDDAQDAAATATTPEAPDAAAPLAERMRPRTF
                     DEFVGQRQLVGEGRFLRRIIEGEGMLPSLI"
BASE COUNT          710 a         1553 c         1665 g          808 t
ORIGIN      
        1 atgatgatgc cgacgccgga gtcgcggtca acgccgggcg cctcgatgtc gatggccgag
       61 ccgttgagcg ccgcgcgcat ctgcgcctgc gtgatcgagg ggacgccgtc cttgacgagg
      121 gccgcgatgg ccgccgcgtg cggcgccgcc gccgaggtgc cgaagaacgg gttggggaag
      181 ccgcccgcgc cggagacgac gccgccgtca gccgccgtga tgtcgggctt ctggcgcgtg
      241 atgccgccgg tggcgaggaa gtcgccgggc acgtagggcg tgccgttggg ctggaagtag
      301 agcttgcgcg ggccgtcgga ctcgaacgtc tcgaccatgt tcgtcgagtt gaacggcatc
      361 gggaaggggc cgacggcggg cgtcgccgcg acggcgtagg ccagggccgc gctggcgtgg
      421 ccgtgggtct ggcccgcggt ggcgaagagg tagcgcccgc ggaaggtgtt ggtgtggagg
      481 aagcggtcgg cgcccgagaa cttggcgacg acgacgcggt tgttcgtcac gttggcgccg
      541 ccgccgacaa gctcgaacgg gtcttgggtg ccgtcctgcg agccggtcga ggccgcgacc
      601 agcgcggtgc ccgccgcgtt caggatgtag aggtcgtagt cgttgttgga ggcgccgagc
      661 gggtcggccc aggtgagggt gatcgggttg ccggagccga gcgtgatgcg gttgaactgc
      721 gagaccgccg ccgtggggtc gaagtcgtgg acggggccgg cgcccgcacc gccgatgacg
      781 gggatcgtgc cgccgttgac gaagttgccc tcgaagacgc ccgacgtgcc gtcgttgagg
      841 ttgccctcgt tgccggcgga cgagaagtac tcggcgccgt cggcggtcac gtcgttgacg
      901 gcctgcgtga cgacgccgcc gttggtgttc gagatgatgg acggggcctg cccgtcctgg
      961 aagaccgcct cggcgaagta gaacacgtcg tccacgatga tgtcgcagtc gtagtcgtcg
     1021 cgcagcgtgc ggatgttgtc ggcgaacgag gtgatgccgc cgaaggcggt ggcgaagaag
     1081 agctgcgcgc cgggcgcgag gtcgtggact atctcaagca tcgccgtgcc ctcgtcgccg
     1141 gagccggcct ggccgggcag cacggtcact tcccccaagt cgcccgacgc ctgcgcgaag
     1201 gagaggttga cgacgccgtc ggagagaacg ccgatcttga cgcccgtgcc gtcaacgttg
     1261 tagcgacggc gcgccacgtc ggcgcggtgc gtcacgtcgc cctcggcgcg gcgcgcgccg
     1321 actgagttga cgccgacgcc gccgtcggcg ggcgagtcgt cggtgcggta agcggcgagc
     1381 gcgctcgaca gcttctcgcg gacgcgcgcg gcgcgcgcag cgaagtcggg gcgcaaggcg
     1441 ctcgcgggat tcccggcccc gatcggcacg tcgaggccgg cgttggcgcg ctcgcggcgc
     1501 gaggtcatgg cctcctgctt cggctggatg tagcgcacgt cgggcgacgc ggcgatggtc
     1561 tccagttgtt cgagcgcgac ctccgcgcgg aggctgccgt aggcgggcga cgcgtacagc
     1621 acgcgcgcgc cgttggcttc gagcgtttgc agcaacgcgt cgccgaccgt gcccgagatg
     1681 tccacgacga cgcggccccg ctcggtctcg cccacgctca ccgagagcga ctggacgccc
     1741 gcggcgatag gctcgccgcg actcatcttc gacgcgtaga ggagctgcga gtcgatcttc
     1801 cgctgcgccg gggtgcgcga acgcttctcc gcggcgagcg ccccgatctg cgccgccgcg
     1861 ctcgcgctga tgcccttgtc tgcggcagcc gcgtcctgcg cggcctcggc gcggcgggca
     1921 ccgccgggga agagcgtgct gccggcggcg agggcagtga tgccgacggc cagcaacgat
     1981 gacagtaaga gagcgcgccg cgcgtggctc cgcgacgcgt tggcgcgacc tgtggtcggg
     2041 cgagcggcac cgtgatttct tcggcctgtg ttcattaact caactcctcc ttaaccttca
     2101 tgcgaacgag gttgaagctg gagaacggag caagtgcagc cccatcccgg aagcagtcgg
     2161 acggcgtgtg tcaagcgtca aatgtctcgg gaaaggcgcc ggcttccggg gggaaaccgg
     2221 cgacgagtcg gcgcggactt gccttgcgcc ggcccgtcag ttggttcgtg cgccgccgtg
     2281 ggtcatcgcc tgggcggcgg gcgagatgta tctgacttct tctattgccg ctagggcttc
     2341 gagcttttcg agcgtcacct tgacgcggac ggtgtgatac ttcgcggact gtgagacgac
     2401 ctccgcgccg agcttgccga gtttcgagat gagtttcgcc gacaccctcg cggtgatgtc
     2461 cacgagcgct cgaccgtgcg cgtcgagctt gatgtctacc ggctcggagg ggacgccgcg
     2521 ccgctcgccg cgcttctgct tgagcgcgta gagcagttgc gtgtcgatct tcttctgcgc
     2581 cgccgtgcgc gcccttttct cgcgcgcgag gtcggcaatc tgtcgtgcgg cttcgggact
     2641 caccttctga tcctgcgact gcggcggcgc gggcggtgtc gtctgcgccg cggcggcgag
     2701 tggcgtcgcc gcggagacga cggcgagcgc caccagagac caaagcggca acttctcaca
     2761 acgcttcatg ggttcaatcc cttgcaacgg actgaagtgg ttcgaatcca gcggcgcggc
     2821 ccgacagtgg cccttccggt gtcggcgtgt gcgaaggaaa atagttcgtt ccctccccga
     2881 agtcaatccg ttagacacct gaccgcttgt atgtttgtcg tgagtcgtga ggacgcagtg
     2941 cccggttcga taaggctatc gtgcgcgagc ggcatgaagg taaaggcggc gagacgtaag
     3001 cagcgccgca cactccttct ccgaatcaaa attcggaaga tacgtcagca ggttttgcgt
     3061 gtctccgacg ggggcacgga aggggtgaag ttcgcttcgg cgcatggctc agcccgtggt
     3121 cgattcaggg ggcgggcgtg accttcttcc actgctcggg gttctccgcg cccttgccgt
     3181 cgcagagcgt gtcggcgcgc agttccttca gcaccggccc ggcgtcatcg aagctgtagg
     3241 tcgagagcgt gccgcagtcg cccggcccgc ggaagcggtt gtggactttc aactgcttcg
     3301 tccgcgcgtc gaaatcggcg agtccccaca actccgtcgc gctggtcttt tcgagtttgc
     3361 cgtcttccgg cgattcgtga acgtcgaagg tcaggacttt cgcggagggc gtcgcccccg
     3421 cctcgttcac gacggcgaag acctgcgacg gctgatatgc gccgcccgcg cacagcacct
     3481 tcaagaggcg gcgcccttcg cccaattcga agaactcaag accggcgttg tcgcccgcac
     3541 gccccgccgt ccccgcgaaa gcctcttcgc attcctccgg ccagcgaatg atctcgcgcc
     3601 acgccttgcg gtcggcgggc gtcagcttgc cctcggtcgg cggcagcgac gcgcgcgcag
     3661 ccgtcggcgg cgtcggcgaa ggtttgcccg cggtcgtcgt ctccgcctta gccgccgcgg
     3721 cttctttcgc gttgccgcgt cccgcgttct cttgcggcgc gcacgcgacg gcgaagagcg
     3781 ccgccggcaa aagcacgagc gaccgcgccg tcgtcttcgt catctttctt gccacgaaca
     3841 gcatccgctc cgaacccgcg ccgccgaaga tgttgaccac tctcgtcgga cgacgacgcc
     3901 gggatgaacc gaccgcaaaa gaaaagcgtt gcccggacac tttatcgtcc gggcaacgcg
     3961 aatggttcaa gaaagcttta caggagtttg gccgtccgcg ccgccgcccc gtgcgtgcgt
     4021 gcggttcaac gcagcacggc gcgcacgccg acgaggacga ggtagccgat ggcgaggaac
     4081 actgccagct tgataagcag gacggcgagt ttgacgaccg tcccgacgac gatgccagcg
     4141 gcggtcagta cgagcggcac cacgaccgcc agcacgaaca tgccgaggag tatgattccg
     4201 acgactctca acaggtcttt gacgtctctc atgatttcac ctctatcatc tcgccgcctt
     4261 attcggtatt acgtggcggc gctgtctcgc ctccgtatac gccgcagcga gagggcgtgt
     4321 tccgtacaag atattacacc cgagccccgc tttttgtatc gccctttgtc cccgcctgtc
     4381 accacttttt tcgaattatg ttaagctctt cggcatcccc ttgcaactca cgcgcgcacg
     4441 cgggcgaccc gaggcaagcg cctttgagcg cgggccgcgc gcgcccggcg cggggggcga
     4501 acgggtgata cgaatggcac gcccacgacg cacgaccgac aaacttttcg acgacgacgc
     4561 gcaagacgcg gcggcgacgg ccaccacgcc cgaggcgccg gacgccgccg cgccgctcgc
     4621 cgagcgcatg cgcccgcgca cgttcgacga gttcgtcggc cagcgccagc tcgtcggcga
     4681 gggccgcttc ctgcgccgca tcatcgaggg cgaagggatg ttgccttcac tcatcc
//
LOCUS       DBNGOH010000909         1617 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_137462, whole genome shotgun sequence.
ACCESSION   DBNGOH010000909 DBNGOH010000000
VERSION     DBNGOH010000909.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1617)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1617)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1617
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_137462"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..476
                     /locus_tag="AC159P_17710"
     CDS             <1..476
                     /locus_tag="AC159P_17710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002615897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HIR3697333.1"
                     /translation="GRFHFLGFIPRKGAERRAMLEEVAQLSATLVLYESPRRVGETLP
                     DLLDAWGDRRACVARELTKLHEEFARGRLGELAAHFGGEQAARGEIVLVIDRNVIADA
                     SEQGAGAGVVAERVAAFEAEGLDQRAALKRAARELGLTRDEAYRRLTSERARAKG"
     gene            575..>1617
                     /locus_tag="AC159P_17715"
     CDS             575..>1617
                     /locus_tag="AC159P_17715"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014081.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR3697334.1"
                     /translation="MPNPLTPTPRSILRVVLILVAVSLNALLPAGARQQQPPPGADDT
                     LAIVGATVVDGTGREPFRGTVLVRGGRIAAVGREVETPAGARVVRAEGRTLVPGLFDL
                     HTHLPYATAPGITGDWPKNLKAYLYCGVTSVVEFGTYPETFEPVRRLVSRGAVEGPRI
                     HFAARMSTPGGHGVEGGRGDLFTLEVQTPREARAAVRRVLPYKPDAIKVFTDGWRYNT
                     APDMTSMNEETLAAIVEEAHKHGVEVLTHTVTLERAKVAARAGVDVIAHGVGDRVVDG
                     ELAELMRAMGTTYVSTLAVYEPRGRDILTPLLSAVLEPAVRAQVSPPLTEPPAAGENV
                     GALPPEPTAATSA"
BASE COUNT          241 a          596 c          569 g          211 t
ORIGIN      
        1 cggggcgctt ccacttcctg ggcttcattc cgcgcaaggg ggccgagcgc cgggccatgc
       61 tggaggaggt ggcccagctg tccgccacgc tggtgctcta cgagtccccg cgccgggtgg
      121 gtgaaacgct gccggacctg ctggacgcct ggggagaccg gcgcgcctgc gtggcccgcg
      181 agctgacgaa gctgcacgag gagttcgccc gcggacgcct cggcgagctg gccgcgcact
      241 tcggcggaga gcaggcggca cgcggcgaga tagttctggt catcgaccgc aacgtcatcg
      301 ccgacgcgtc ggaacagggc gcgggcgcgg gcgtcgtcgc cgagcgcgtg gccgccttcg
      361 aggccgaagg actcgaccag cgcgcggccc tcaagcgcgc cgcgcgcgaa ctcggtctta
      421 cgcgcgacga ggcttaccgc cgcctcacgt ccgagcgcgc gcgggcgaaa ggctgaaggc
      481 cgtgcttctt gtcgcccgcc gcgccttcat ctatgctcga cgctcgaccc gaaagaaatc
      541 caacaacgca tcacgactac ggtctcgaca tccgatgccc aacccgctaa cgccgacgcc
      601 gcgttcgatc ttgagggtcg tgctgatcct cgtcgccgtc tcgctcaacg cgctgctccc
      661 ggcgggcgcg cggcagcaac aaccgccgcc gggcgccgac gacacgctcg ccatcgtcgg
      721 cgcgaccgtc gttgacggca cggggcgcga gccttttcgg gggacggtgc tggtgcgcgg
      781 cgggcgcatc gcggcggtcg ggcgcgaggt cgagacgccc gcgggcgcgc gcgtcgtccg
      841 cgccgaaggg cggacgctcg tgcccggtct cttcgacctg cacacacacc tgccttacgc
      901 gaccgcgccg ggcatcacgg gcgactggcc gaagaacctc aaggcgtacc tctactgcgg
      961 cgtgacgagc gtcgtcgagt tcggcaccta cccagagacg ttcgaacccg tgcggcgcct
     1021 tgtctcgagg ggagcggtcg aagggccgcg catccacttc gccgcgcgca tgtccacgcc
     1081 cggcggccac ggcgtcgaag gcggtcgcgg cgatctcttc acgctcgaag tgcagacgcc
     1141 gcgcgaggcg cgcgccgccg tccgccgcgt gctgccctac aagcccgacg cgatcaaagt
     1201 cttcaccgac ggctggcgct acaacaccgc gcccgacatg acgagcatga acgaggagac
     1261 gctcgccgcc atcgtcgaag aggcgcacaa gcacggcgtc gaggtgctga cgcacaccgt
     1321 cacgctcgaa cgcgcgaagg tggccgcgcg cgcgggcgtg gacgtgatcg cgcacggcgt
     1381 cggcgaccgc gtggtagacg gcgaactcgc cgaactcatg cgtgcgatgg gcacgaccta
     1441 cgtctcgacg ctcgccgtct acgagccgcg cgggcgcgac atcctgacgc cgctcctctc
     1501 cgccgtgctc gaacccgccg tgcgtgcgca ggtctcgccg ccgctcaccg agccgcccgc
     1561 cgcgggcgag aacgtcggcg cgctgccgcc cgagccgacg gcggcgacga gcgcgaa
//
LOCUS       DBNGOH010000910         5717 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4168848, whole genome shotgun sequence.
ACCESSION   DBNGOH010000910 DBNGOH010000000
VERSION     DBNGOH010000910.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5717)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5717)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5717
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4168848"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1855
                     /gene="infB"
                     /locus_tag="AC159P_17720"
     CDS             <1..1855
                     /gene="infB"
                     /locus_tag="AC159P_17720"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HIR3697335.1"
                     /translation="ERIAVSLNVGAGGATATLKPVRLVEGSTVKEFAEKLGIKPKDVV
                     ALLLQRGVFATINQPLKTDVAKDLGQRFGFDVSFVDFEEMVAEEEIDEIIGGDSMGDY
                     VEEARAPVVTVMGHVDHGKTSLLDAIRTTNVAAGEAGGITQHIGAYSVQVASPDAPDQ
                     TRRVVFLDTPGHEAFTMMRARGAKATDVVVLVVAADDGVMPQTVEAIEHSRAAGVPIV
                     VAINKIDRPDANPDRVKQELAVQGLQPVDWGGDIEMVAVSAKQRQNIETLLETILLTA
                     DILNLRASPTRLASGVVLEANLDRGRGSVATVLVQQGTLRVHDPFIVGQIFGKVRAMY
                     NDRGEQITEAGPATPVEVLGLQGVPQAGEAFQVVAEITKAQQISHHRQMISRQNTLLQ
                     TTKRGIEALGEREVKELLLIVKADVQGSVEVLKSTLTKLSTDAVKVRVVRSGVGAITE
                     SDVLLAAATQTEGSGRAVSIIGFNVRPEVRAGELARQEHVDIRLHSIIYKLEEEIRAA
                     MIGMLEAIEKEKIIGTALVQEVFKVPRIGAIAGCRVTNGTIRRNARARLIRDGVVSWE
                     GNIASLRRFKDDVGEVREGFECGIGLENFSDVKANDQIEAYVIEKVAATEL"
     gene            1941..2339
                     /gene="rbfA"
                     /locus_tag="AC159P_17725"
     CDS             1941..2339
                     /gene="rbfA"
                     /locus_tag="AC159P_17725"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HIR3697336.1"
                     /translation="MRRPEQLAELIREEVSQIVGYELDDPRVASVTVTDVRVVENLRD
                     ARVFVTAEGTEEERVAALKALQKAAPYVRRQLGMLLNLKYTPEIHFVRDTVEESATRV
                     EALLSEIAQDNSQQHSPEDEAGDPREEIEP"
     gene            2414..3457
                     /locus_tag="AC159P_17730"
     CDS             2414..3457
                     /locus_tag="AC159P_17730"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013528.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HIR3697337.1"
                     /translation="MLSQVVELIESKRRFAITSHVRPDGDSLGSSLGLYWLLRALDKE
                     PEVIMRDPVPLAYQKLPGAELVRVTPRVDSPYDAVFVIECSDVSRPGLIDLEKQFVVN
                     IDHHSTTALFGTINWIDSTASAVGEMIYNLCKAIGVRVTREIAECVYTALVTDTGSFH
                     YSNTTERTFKVASELVRAGVKPAKVSQAVFGSYPWSKVELLREVLASARRDASGRVAW
                     LRQTIEMQERAGASDEDGDGFVNYPLSCGEVEAVAFLKESAPGLYRVSLRSKCDVNVA
                     RIAEKFGGGGHRNAAGCMLRGSWEQAEETIVQLLVDEVEHRGNGDRHAPLNGAAPREV
                     LPETARAERDARP"
     gene            3584..4552
                     /gene="accD"
                     /locus_tag="AC159P_17735"
     CDS             3584..4552
                     /gene="accD"
                     /locus_tag="AC159P_17735"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015258033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HIR3697338.1"
                     /translation="MSWFRRKNQRIEHVPADERRVKTEGVFEKCPECEAALYRPELEE
                     SLHVCPHCSHHFRVGARIRLAALYDEGKYEELDAEVISSDPLEFTDTKPYKERLEHAR
                     RTSGLPEAVLNARGHIGGHLVLMSAMDFAFLGGSMGTAVGEKITRTVERAISERGSVV
                     IISQSGGARMQEGTLSLMQMAKIAAALARLDAARLPYVSVLTDPTTGGVTASFAMLGD
                     INIAEPKALIGFAGPRVIEQTIRQKLPKDFQRSEFLLDHGMLDAVVDRREMRDFIIKA
                     LDFMDNPAVQFQPLRAHPPSGNGTGPARLDQPTETKAESPEPEAQS"
     gene            4684..>5717
                     /locus_tag="AC159P_17740"
     CDS             4684..>5717
                     /locus_tag="AC159P_17740"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HIR3697339.1"
                     /translation="MTFDEALRYLLGLGHETLAMKLGLDNVARLLARLGEPQRAYPSV
                     QLAGTNGKGSAAAMLEAMARAAGLRTGLYTSPHLVSITERIKIAGREIARESFAREAT
                     VVREAAEQLEREAGALPTFFEQVTAIALAAFRAEGVELAVLETGLGGRLDATTAAAAR
                     VVAVTPVALDHQEYLGHTLAEIAAEKAAIIRPGVRAVLAPQPPEAEEVILARCRECGV
                     EPRHTAKDVKVEGACGEGRLRVSLKTERDEYRGVCLALRGRHQIVNAGVAVALAETLR
                     EEGFAISREHVVAGLESAEHAGRLELDASGAPRVLYDGAHNEAGARALRAYLDEFVEA
                     PVTLVFGAMR"
BASE COUNT         1021 a         1941 c         1893 g          862 t
ORIGIN      
        1 agagcgcatc gccgtgtcgc tgaacgtcgg cgccggcggg gcgaccgcga cgctcaaacc
       61 cgtgcgactc gtcgaaggct cgaccgtcaa agagttcgcc gaaaaactgg gcatcaagcc
      121 gaaggacgtg gtggcgctgc tcttgcagcg cggcgtcttc gccaccatca accagccttt
      181 gaaaaccgac gtggcgaaag acctcggcca gcgcttcggc ttcgacgtgt cgttcgtgga
      241 cttcgaggag atggtggccg aggaggagat cgacgagatc atcggcggcg acagcatggg
      301 cgactacgtc gaagaggcgc gcgccccggt cgtcaccgtg atgggccacg tcgatcacgg
      361 caagacctct ctcctggacg ccatccgcac gaccaacgtc gcggcgggcg aggccggcgg
      421 catcacgcag cacatcggcg cgtacagcgt gcaggtggcc agccccgacg cgcccgacca
      481 gacgcgccgc gtcgtcttcc tcgacacgcc cggccacgag gcgttcacga tgatgcgcgc
      541 gcgcggcgcg aaggcgaccg atgtggtcgt gctcgtcgtc gcggcggacg acggcgtcat
      601 gccgcagacc gtcgaggcca tcgagcactc gcgcgcggcg ggcgtgccca tcgtcgtcgc
      661 catcaacaag atcgaccgcc cggacgccaa ccccgaccgc gtcaagcagg agctggccgt
      721 gcagggcttg cagccggtgg actggggcgg cgacatcgag atggttgccg tctctgccaa
      781 gcagcggcag aacatcgaga cgctgctcga aaccatcctc ctcaccgcgg acatcctgaa
      841 cctgcgggcc agcccgacgc gcctcgcctc gggcgtcgtg ctcgaagcga atctcgaccg
      901 cgggcgcggc tcggtcgcca ccgtgctcgt ccagcagggc acattgcgcg tccacgaccc
      961 gttcatcgtc ggccaaatct tcggcaaggt gcgcgccatg tacaacgacc gcggcgagca
     1021 gatcaccgaa gcaggcccgg cgactcccgt cgaagtgctc ggacttcagg gcgtgccgca
     1081 ggcgggcgag gcgttccagg tcgtcgccga gatcacgaag gcgcagcaga tttcgcacca
     1141 ccgccagatg atttcgcggc agaacacgct ccttcagacc accaagcgcg gcatcgaggc
     1201 tctgggcgag cgcgaggtca aggagctgct gctcatcgtc aaggccgacg tgcagggttc
     1261 ggtcgaggtg ttgaagagca cgctcaccaa actctcgacc gacgcggtca aggtacgcgt
     1321 cgtgcgctcg ggcgtcggag ccatcaccga gtcggacgtg cttctggccg ccgcgacgca
     1381 gaccgagggt tcgggccgcg ccgtctccat catcggcttc aacgtgaggc ccgaggtgcg
     1441 cgccggggag ctggcccggc aggagcacgt ggacatccgc ctgcactcga tcatctacaa
     1501 gctcgaagaa gagattcgcg cggcgatgat cggcatgctc gaagccatcg agaaggaaaa
     1561 gatcatcggc acggcgctcg tgcaggaggt cttcaaggtg ccgcgcatcg gcgccatcgc
     1621 cggctgccgc gtcaccaacg gcacgatccg ccgcaacgcc cgcgcccgcc tcatccgcga
     1681 cggcgtcgtc tcgtgggagg gcaacatcgc ctctttgcgc cgcttcaagg acgacgtggg
     1741 cgaagtccgc gagggcttcg agtgcggcat cggcctcgaa aacttcagcg acgtgaaggc
     1801 caacgaccag atcgaggctt acgtcatcga gaaggtggcg gcgacggaac tgtaaagagg
     1861 gtgttgggtt ttgggtgtta ggtgttggct tgatgattta accaacaccc aacacctaac
     1921 acccaaaacc cgtttttgtt atgcgaaggc cggaacaact cgccgagttg atacgcgagg
     1981 aggtcagcca gatcgtgggc tacgagctgg acgacccgcg ggtggcgtcc gtgacggtga
     2041 cggacgtgcg cgtcgtcgag aacctgcgcg acgcgcgcgt cttcgtcacc gccgagggca
     2101 ccgaagagga gcgcgtcgcc gcgctcaagg cgctccagaa ggccgcgccc tacgtgcgcc
     2161 gccagctcgg catgctcctc aacctcaagt acacgcccga gattcacttc gtccgcgaca
     2221 ccgtcgagga atcggccacg cgcgtcgaag cactgctgtc agagatcgca caggacaaca
     2281 gccagcagca ctcccctgaa gacgaggctg gcgacccgag agaggagata gaaccgtaaa
     2341 ccgtaaaccg tgacaagata agacctgctt tcctcttgtc acggtttacg gtttacggtt
     2401 cacggctttt gatatgctga gtcaagtcgt tgagctgatc gagtcgaagc gccgcttcgc
     2461 gatcacgtcg cacgtacgcc cggatggtga cagtctgggt tcctcgcttg gtttatattg
     2521 gctgctccgg gcgctcgaca aagagccgga agtcatcatg cgcgacccgg taccgctcgc
     2581 ctaccagaag ctgccgggcg ccgaactggt ccgcgtcacc ccgcgcgtcg attcgcctta
     2641 cgacgccgtc ttcgtcatcg agtgctcgga cgtttcccgc cccggcctga tcgacctcga
     2701 aaagcagttc gtcgtcaaca tcgaccacca ctcgacgacc gcgctcttcg ggacgatcaa
     2761 ctggatcgac tcgaccgcct cggcggtcgg cgagatgatc tataacctct gcaaggccat
     2821 cggggtgcgc gtcaccagag agatcgccga gtgcgtctac acggcgctcg tcaccgacac
     2881 cggctcgttc cactactcga acacgaccga gcgcacgttc aaggtcgcgt ccgagctggt
     2941 gcgcgccggg gtcaagccgg cgaaggtctc gcaggccgtg ttcggcagct acccgtggag
     3001 caaggtcgag ctgctcaggg aagtcttggc gagcgcgcgg cgcgacgcct cggggcgggt
     3061 ggcgtggctg cggcagacca tcgagatgca ggagcgcgcc ggggcttcgg acgaggacgg
     3121 cgacggcttc gtcaactacc cgctctcgtg cggcgaggtc gaggccgtcg cgttcctgaa
     3181 ggagagcgcg ccgggcctct accgcgtctc cttgcgctcg aagtgcgacg tgaacgtggc
     3241 gcgcatcgcg gagaagttcg gcggcggcgg ccaccgcaac gccgccggct gcatgctgcg
     3301 cgggagctgg gagcaggccg aggagaccat cgtccaactc ctggtggacg aggtggagca
     3361 ccgcggcaac ggcgaccgcc acgccccctt gaacggcgcg gcgccgcgcg aggtgctgcc
     3421 cgagacggcg agggccgagc gcgatgccag accttagaag cagtggtcag tggccggtgg
     3481 tcagtggtcg gtagttgacc gtccggcggc tcgacgtttc gggccgcgac ttgactgctc
     3541 acgcaaaagg ttctttactg accactgacc actgaccact gacatgtcct ggttccgcag
     3601 aaagaatcaa cggatcgaac acgtcccggc ggacgagcgc cgcgtgaaga ccgagggcgt
     3661 gttcgagaag tgccccgagt gcgaggccgc cctctaccgc ccggaactcg aagagtccct
     3721 gcacgtctgc ccgcattgca gccaccactt ccgcgtcggc gcgcgcatcc gcctcgccgc
     3781 cctctacgac gaaggcaagt acgaagagct ggacgccgag gtcatctcca gcgacccgct
     3841 cgaattcacc gacaccaagc cctacaagga gcggctcgaa cacgcgcggc gcacttcggg
     3901 cctgcccgag gccgtcctca acgcgcgcgg ccacatcggc ggccacctcg tcctgatgtc
     3961 cgcgatggac ttcgccttcc tcggcggctc gatgggcacg gcggtcggcg agaagatcac
     4021 gcgcaccgtc gagcgcgcca tcagtgagcg cggctcggtc gtcatcatca gccagtcggg
     4081 cggcgcgcgc atgcaggagg gcacgctcag cctgatgcag atggcgaaaa tcgccgcggc
     4141 gctggcgcgc ctcgacgcgg cgcggctccc ttacgtctcg gtcttgaccg acccgaccac
     4201 gggcggcgtc accgccagct tcgccatgct cggcgacatc aacatcgccg agccgaaggc
     4261 gctcatcggc ttcgccggcc cgcgcgtcat cgagcagacc atccgccaga agctccccaa
     4321 ggatttccag cgctccgagt tcctgctcga ccacggcatg ctcgacgccg tcgttgaccg
     4381 ccgcgagatg cgcgacttca tcatcaaggc gctcgacttc atggacaacc ccgccgtcca
     4441 gttccagccg ctccgcgccc atcccccttc gggcaacggc accggccccg cgcgtctcga
     4501 ccagccaacc gagacgaagg ccgaaagccc tgagccggaa gctcaaagtt agacctcctc
     4561 caagtcattt cggatttcgg atttcggatt agaagaagca ttgacggttg acgggagcgg
     4621 cgctgtcatc aatcgtcagc cgttaatccg aaatccgaaa tccgaaatcc aaaatccgca
     4681 accatgacgt tcgacgaagc gctccgctac ctgctcggtc tcgggcacga gacgctggcg
     4741 atgaagctcg gcctcgacaa cgtcgcgcgg ctgctcgcgc ggctcggcga gccgcagcgc
     4801 gcctacccct cggtgcagct cgcggggacg aacggcaagg gaagcgccgc cgcgatgctc
     4861 gaagcgatgg cgcgcgcggc gggcctgcgg acagggcttt acacttcgcc gcatctggtc
     4921 tccatcaccg agcgcatcaa aatcgcgggg cgcgagattg cgcgcgagag cttcgcgcgc
     4981 gaggcgaccg tcgtgcgcga ggccgccgaa cagctcgaac gcgaggcggg cgcgctgccg
     5041 accttcttcg agcaggtgac ggccatcgcg ctcgcggcgt tccgcgccga gggcgtcgaa
     5101 ctcgccgttc tggagaccgg cctcggcggt cggctcgacg cgacgaccgc ggctgccgcg
     5161 cgcgtcgtcg ccgtcacgcc cgtcgcgctc gatcatcagg aatacctcgg ccacacgctc
     5221 gcggagattg ccgccgagaa ggccgccatc atccgccccg gcgtgcgcgc cgttctggcg
     5281 ccgcagccgc ccgaggccga agaggtcatc ctcgcgcgct gccgcgagtg cggcgtcgag
     5341 ccgcggcaca ccgcgaagga tgtgaaggtc gaaggcgcgt gcggcgaagg ccgtctgcgc
     5401 gtgagcctga agaccgagcg cgacgagtat cgcggcgtct gtctcgcgct gcggggtcgt
     5461 catcagatcg tgaacgcagg cgtggccgtc gcgctcgccg agacgctgcg cgaagagggc
     5521 ttcgccatct cgcgcgaaca cgtcgtcgcg gggcttgaga gcgcggagca cgcgggccga
     5581 ctcgagctgg acgcgtcggg cgcgccgcgc gtgctctacg acggcgcgca caacgaggcg
     5641 ggcgcgcgcg cgctgcgcgc gtacctcgac gagttcgtcg aggcgcccgt gacgctcgtc
     5701 ttcggcgcga tgcgcga
//
LOCUS       DBNGOH010000911         2566 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4536224, whole genome shotgun sequence.
ACCESSION   DBNGOH010000911 DBNGOH010000000
VERSION     DBNGOH010000911.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2566)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2566)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2566
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4536224"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..470)
                     /locus_tag="AC159P_17745"
     CDS             complement(<1..470)
                     /locus_tag="AC159P_17745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697340.1"
                     /translation="MQRLAAALTLSAATALFLFLPASAKGKARPGPSVVAGARRLPDV
                     SFARRAKRGCDRYVSTAGDDANSGRSVRGAWRTIGMALRTLRAGQVGCVLPGTYEEGH
                     NEAGHDGTAAGPIVLRRAPGSRAMPVVKPKGVVAVFHIDRDYWVLDGFDVDVDF"
     gene            complement(501..2303)
                     /locus_tag="AC159P_17750"
     CDS             complement(501..2303)
                     /locus_tag="AC159P_17750"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HIR3697341.1"
                     /translation="MKLPKRVYAISLALALMTAGVLLDSRYPREATAQSLPAAKRFEV
                     AVENGVRVKMRDGVTLVADVYRPKGEGKFPVLVERTPYNRKGDFAMAHALAAHGYVVV
                     LQDTRGRFDSGGEFYPFRHEAEDGYDTVEWAAALEQSDGKVGMFGGSYVGATQMLAAM
                     AKPPHLAAIFPYVTASEYYDGWTYQSGALMQWFASSWTTGLTVDTLRRKTAGRSRPKE
                     WVGQLPLENYKLLDPPPVADLAPYFRDWLRHERDDEYWRRWKVSAHYREMNVKALHAG
                     GWNDLFIKGSIKNYTGLRAEGTTASVRDGQRLLVGPWAHAATSPEGKVGDVVYGRAAV
                     LDMTGTALRWFDFALKGVQNEYATAPPVSIFVMGENVWRAEREFPLARTRYTKYFLHS
                     ARGANGVEGDGRLDTLAPAAERPDAFTYDPLNPVPTIGGRLCCGDAIPPGPFDQRPNE
                     SRKDVLVFSTPPLERDLEVTGYVTAELYAATSAPDTDFTMMLVDVAPDGYARYLTDGI
                     VRARYRDTTDRADPVTPGRVYKYTLDLWATSNLFKAGHRIRLYVSSSNFPRFNRNHNT
                     GETSAAAARAAKAEQTVYHDASRPSSVMLPVIPR"
     gene            complement(2309..>2566)
                     /locus_tag="AC159P_17755"
     CDS             complement(2309..>2566)
                     /locus_tag="AC159P_17755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697342.1"
                     /translation="CFAKPGEPGGELGPDMEMHNPEMAYTFALGAFFTKDPARRAAYR
                     RRFDRLFPLAVKLDVNKPPRAFSWVFQFTSQLVYFMQRAGR"
BASE COUNT          373 a          857 c          897 g          439 t
ORIGIN      
        1 tgaaagtcga cgtccacgtc gaagccgtcc agcacccagt agtcgcggtc gatgtggaag
       61 accgccacga cgcctttcgg tttgacgacg ggcatggcgc gggagccggg cgcgcgtctg
      121 agtacgatgg gcccggcggc ggtgccgtcg tgcccggcct cattatgccc ctcttcgtat
      181 gttcccggca gcacgcaccc gacctgcccg gcacggagtg tgcgcagcgc catgcctatc
      241 gtccgccacg cgccgcggac gctgcgcccg gagttcgcgt cgtcgcccgc cgtcgagacg
      301 tagcggtcgc agccgcgttt ggcgcgccgc gcgaagctga cgtcgggaag cctgcgggcg
      361 cccgcgacca cgctcgggcc ggggcgcgcc tttcctttgg ctgaggcggg caggaagagg
      421 aacaaggccg ttgcggcgct caacgtgagg gctgcggcta atctctgcac ttgatatctc
      481 cgtagttcgc gccgcgccgg ttaacgcggg atgacgggca gcatgacgct cgatggccgc
      541 gacgcatcgt ggtagacggt ctgttcggcc ttggcggcgc gcgccgcagc cgcgctcgtc
      601 tcgcccgtgt tgtggttgcg gttgaagcgc gggaagttgc tgctcgacac gtagagccga
      661 atcctgtgtc cggccttgaa gaggttgctc gtcgcccaga ggtccagagt gtacttgtag
      721 acgcgcccgg gcgtgacggg atcagccctg tcggtagtgt cgcggtagcg cgcgcggacg
      781 atgccgtccg tgaggtagcg cgcgtagccg tcgggtgcga catcgacgag catcatcgtg
      841 aagtccgtgt cgggcgccga cgttgcggcg tagagttccg ccgtcacgta acccgtcact
      901 tccaagtccc tctccagcgg cggggtcgag aagaccagca cgtccttgcg cgactcgttc
      961 gggcgctggt cgaacggccc cggcgggatg gcgtcgccgc agcagaggcg tccgccgatg
     1021 gtcggcaccg ggttcagtgg gtcgtaggtg aaggcgtcgg ggcgctccgc ggcgggcgcg
     1081 agcgtgtcga gcctgccgtc gccctcgacg ccgttggcgc cgcgcgccga gtgcaggaag
     1141 tacttcgtgt agcgcgtgcg cgcgagcggg aactcgcgct ccgcgcgcca gacgttctcg
     1201 cccatgacga agatggagac gggtggcgcc gtggcgtact cgttctgcac gcccttgagc
     1261 gcgaagtcga accacctgag cgccgtcccg gtcatgtcga ggacggctgc cctgccgtag
     1321 acgacgtcgc cgaccttgcc ctcgggcgag gtcgccgcgt gcgcccacgg gccgacgagg
     1381 agccgctgcc cgtccctgac ggacgcggtg gtgccctcgg cgcgcaggcc cgtgtaattt
     1441 ttgatcgagc ctttgatgaa gaggtcgttc cagccgccgg cgtgcagcgc cttcacgttc
     1501 atctcgcggt agtgggccga gaccttccag cgccgccaat attcgtcgtc ccgctcgtgc
     1561 cgcagccagt cgcggaagta gggcgcgagg tctgcgacgg gcggcgggtc gagcagtttg
     1621 tagttctcca gaggcagttg cccgacccac tccttgggcc gggagcgccc cgcggtcttc
     1681 cgacgcagcg tgtccaccgt aagccccgtc gtccaggaac tcgcgaacca ctgcatcagt
     1741 gcgccgctct ggtacgtcca gccgtcgtag tactccgacg ccgtgacgta ggggaagatg
     1801 gcggcgaggt gcggcggctt cgccatggcg gccagcatct gcgtggcgcc gacgtaagag
     1861 ccgccgaaca tcccgacctt cccgtccgac tgttcgagcg cggcggccca ctcgaccgtg
     1921 tcgtaaccgt cctcggcctc gtgcctgaag gggtagaact cgccccccga gtcgaagcgt
     1981 ccgcgcgtgt cctgtaggac gacgacatag ccgtgcgcgg ccagcgcgtg cgccatcgcg
     2041 aagtcgccct tgcggttgta gggcgtgcgc tcgacgagga cggggaactt tccctccccc
     2101 ttcggcctgt agacgtcggc caccagcgtc acgccgtcgc gcatcttcac ccgcacgccg
     2161 ttctcgaccg cgacctcgaa ccgcttcgcc gcgggaaggc tctgggcggt tgcttcccta
     2221 gggtatcgcg agtcgagcag gacgcccgcg gtcatcaacg cgagcgcaag ggagattgcg
     2281 taaaccctct tcggcagttt catcattgtc acctccctgc gcgctgcatg aagtagacga
     2341 gctggctcgt gaactggaag acccaactga aggcgcgcgg gggcttgttc acgtcgagct
     2401 tgacggcaag cgggaagagc cggtcgaagc gcctgcggta cgcggcgcgg cgcgcggggt
     2461 cttttgtaaa gaacgccccg agggcgaagg tgtaggccat ctccgggttg tgcatctcca
     2521 tgtccggccc caactcgccg cccggctcgc ccggcttcgc aaaaca
//
LOCUS       DBNGOH010000912         5745 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3071424, whole genome shotgun sequence.
ACCESSION   DBNGOH010000912 DBNGOH010000000
VERSION     DBNGOH010000912.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5745)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5745)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5745
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3071424"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..388)
                     /locus_tag="AC159P_17760"
     CDS             complement(<1..388)
                     /locus_tag="AC159P_17760"
                     /inference="COORDINATES: protein motif:HMM:NF048382.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBM96 family carbohydrate-binding protein"
                     /protein_id="HIR3697343.1"
                     /translation="MTKLITRVSFVLICLSALATGAYGQAVLVSDAHTSATSAGGNFG
                     ANPTLNVSDNNTAYVKFKIARTLPAGTKADDVARATVKFYVSKVATAGRLDVFPVSTD
                     WDEKAITANNAPAVGPLALTTQQIGKD"
     gene            845..3901
                     /locus_tag="AC159P_17765"
     CDS             845..3901
                     /locus_tag="AC159P_17765"
                     /inference="COORDINATES: protein motif:HMM:NF024180.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing tetratricopeptide repeat
                     protein"
                     /protein_id="HIR3697344.1"
                     /translation="MPHTLRTALYSLALSLTLFNPARAQSQSIPEQPAPTKINYLAAA
                     LAGAASEGEQERLLAQKEDLAGGALLAALRTLAEPLVQKGDYAGALRISRLAVRVAER
                     AGDRNGQGDALHHLGLIYSRQHRAAQAVESFQQSLAIFEETGNKRETAQVLLDVGVVH
                     RLQGRFEQALDNFGRSLALGEAIGDKRLTAKALNNIGMVHRNQGRYELALEFYERGRA
                     LGEEINDKTVLRDVASSVGNVYQAQGLYEQALEHYRRGRSLSEEINDKAGVARNTYSI
                     GAVYNFQGRYAEALACFQQSIKIGEELGAAADKRDLAYNLHSLGRLYVSQGRYDQALV
                     HYHKSLKIREEVNDRFGVGQTLNNIGIVYKSQGLYEQALEWFRKRLKLAEEMGVKEGM
                     ATSLSNISDIHRQQGRSDLALEHLRKSLRLREEMSDRRGICVVLNKLARLYQDKGGYH
                     EMLAVGQRAAGLAEEINAAEELWEAREHVGRALRALGQPTEARRNYLAAIATVESLRR
                     GVAGGEQQRQSFLENRLSPWLGMISLLVSQKEGAEALSFAERSKARVLLDALQSGHAG
                     LRRTLSQTELSGEEERRLRLVSLNSLLTSEARRDRPNPARMAALRADVEKARLEYEDF
                     QTRLYVAHPELRVHRGEAPIIKPEELTALLPDASTALLEYVVAADETYLFAVTRAAGK
                     SEAELVVYTMPVGREELARQAGAFRRQLAGRDLGFRAPARKLYDLLLGPAREQLRGKT
                     SLVIAPDNVLWELPFQALLTDANRFLLEEAAIAYAPSLTVLREMTRRRQSHHSGGAPA
                     TLLALGNPLVGKETLNRAALTLRGGGLDPLPEAEQEVKELGRLYGSSRSKVYTGAEAR
                     EDRVKGEAGGSRVLHFAAHATLDDAAPMYSHLALAGGGAGEDGLLEAWELMQLDLRAD
                     LAVLSACETARGHAAAGEGVIGFSWAMFVAGVPSLVVSQWKVESAGTRDLMVNFHQAL
                     LAPPGGRKGRAGKAEALRQAALRLMRSPETGHPFYWAGFVLVGDGG"
     gene            complement(4342..4653)
                     /locus_tag="AC159P_17770"
     CDS             complement(4342..4653)
                     /locus_tag="AC159P_17770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697345.1"
                     /translation="MVELLNHGNVSLAEAVWFVCEDGDFAAAEKNRAFVLAQYFGYLR
                     RDPDAVGFDGRPDPTFEGYHFWLDKLDQFGGDFVRAESARTDSPVSLPLTAVYISYAS
                     F"
     gene            complement(4698..>5745)
                     /locus_tag="AC159P_17775"
     CDS             complement(4698..>5745)
                     /locus_tag="AC159P_17775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697346.1"
                     /translation="GVGIFNANHRLAVGAGNSSAFAPLSAVGVRGNMAVGTNFAQTAV
                     PTDGAIFEGNVGVGTDNPQARLHVAGAVNFTGLRTQENATSPHVIGGFSGNTVGANIR
                     GATIGGGAAATTVNSVQEDFGTVGGGRNNTVTAVNATVGGGSGNTASGDNGSTVGGGI
                     DNRASGLRATVGGGRLNVATGASATVGGGSENRASGNYATVPGGSSNMADGNYSFAAG
                     QRAQALHDGSFVWSDDTTIDPDADAQCVEATRVNVSAAFFLSIENHETGYLAHRAYKV
                     AYGDLPNKPVPLTLQEYLAGGRSIGDGVQVGWATGGGGSIRTGRPSSTSSSGPSASPR
                     STRRGWTTGPSWTL"
BASE COUNT          987 a         1859 c         1927 g          972 t
ORIGIN      
        1 cgtctttgcc gatctgctgc gtcgtcaggg cgagcggtcc aaccgccggg gcgttgttcg
       61 ccgtgatcgc cttctcgtcc cagtcggtcg agaccgggaa cacgtcgagc ctgccggcgg
      121 tcgccacctt gctcacgtag aacttgacgg tggccctggc gacgtcgtcg gccttggtgc
      181 ccgccggcag cgtgcgcgcg attttgaatt tcacgtaagc ggtgttgttg tccgagacgt
      241 tcagcgtcgg gttggccccg aaattcccgc ccgcggacgt ggcggaggtg tgcgcgtccg
      301 acacgagcac ggcctgcccg taagcccctg tggccagcgc tgacaggcag atcagtacga
      361 acgaaactct ggttatcaac ttagtcatac cgactccgta gttcataagt ggctcctctt
      421 cgactggcgg cgaaaacgtc gcctgtcttg tctcatcctt ctatcaactc ggcggccggt
      481 attggccgcc gagaggtgaa gttgccgaac aggctgccag attactgccc gccacaaatt
      541 tttttgcggt cggggggcgc ccgccccgcg catcagaccg accaatctag tcgtgatccc
      601 tgttgtgaac ggattccgcg ttagaggtta gcgggctaga cgcgaccccg gagccgtgca
      661 gcgttgccgg attgcggtgc ccgaaccttg gtcgccccga catcgccgaa ccagcagctt
      721 gccgccggag gcttcgccct ttttatgcag gagtgctgta tgaacgccgc aggttctcag
      781 gcccctcgtg ggtaggcgcg gcggttcaag gatgaaaagg cggggccggc cagaggagtg
      841 ggctatgccg catacgttac gcaccgcgct gtattcgctg gccctgagct tgaccctctt
      901 caaccccgct cgggcacagt cgcaatcaat ccctgaacag cccgctccga caaaaataaa
      961 ctacctggcc gcggcgctgg ccggagccgc ctcggagggg gagcaggagc ggctgctggc
     1021 gcagaaggaa gacctggcag gcggcgcgct cctggcggcg ctgaggacgt tggccgagcc
     1081 gctcgtgcag aaaggcgatt acgccggggc gctgagaatc tcccggctcg ccgtccgggt
     1141 cgcggagagg gccggggacc ggaacgggca gggcgatgcg ctgcatcatc tcgggctcat
     1201 ctactcccgc cagcatcgcg cggcgcaggc cgtagaatcc ttccagcaaa gcctcgccat
     1261 tttcgaggag accggcaaca agagagagac ggcgcaggtg ctgctcgacg tcggcgtcgt
     1321 ccacaggttg caggggcgct tcgaacaggc gctggataac ttcggccgca gcctggcgct
     1381 cggcgaggca atcggggaca aaaggctgac cgccaaggct ctgaacaaca tcggcatggt
     1441 ccacagaaat caggggcgtt acgaactcgc cctggagttc tacgaaaggg gccgggcgct
     1501 cggtgaagag atcaacgaca agacggtgct gagggatgtt gcctccagcg tcggcaacgt
     1561 ctatcaggcg caggggcttt atgaacaagc gctggagcat taccggaggg gccggtcgct
     1621 cagtgaagag atcaacgaca aggcgggtgt ggcgagaaat acctacagca tcggcgccgt
     1681 ttataatttc cagggccgct atgcggaggc gctcgcctgc tttcagcaga gcatcaagat
     1741 cggcgaagag ttgggcgcag ccgccgacaa gagggacctg gcctacaacc tgcatagcct
     1801 cggcaggctc tacgtgagcc agggtcgtta tgatcaggcg ctggtgcact accacaaaag
     1861 cctgaagatc agggaagagg tcaacgacag attcggcgtc ggccagacgc tgaacaatat
     1921 cggtatcgtt tacaaatcac aggggcttta tgagcaggcg ctggagtggt ttcggaaaag
     1981 gctgaagctg gccgaggaaa tgggcgtcaa ggagggcatg gctaccagcc tgagtaatat
     2041 cagcgacatt catcgccagc aggggcgctc cgacctcgca cttgagcacc tccggaagag
     2101 cctccgcctg cgcgaggaga tgagcgaccg gcgcggcatc tgcgtggtgc tgaataagct
     2161 cgcccggctg taccaggaca aaggcggtta ccacgagatg ctcgcggtcg gccagcgcgc
     2221 cgccggcttg gccgaggaga taaacgcggc cgaagaactc tgggaagccc gggagcacgt
     2281 cggccgagcc ctccgcgcgc tgggccagcc cacggaagcc cgccggaatt atctcgccgc
     2341 catcgccacc gtcgaatcgc tgcgccgcgg ggtcgccggc ggcgagcagc agcggcagag
     2401 cttcctcgag aacaggctct ccccctggct cggcatgatc tccctgctcg tctcgcaaaa
     2461 ggaaggcgca gaagccctga gcttcgccga gaggtcgaag gcgcgggtgc tgctcgacgc
     2521 ccttcagtcg ggtcacgccg gcctgcgccg gacgctctcc cagaccgagc tgtccggcga
     2581 ggaggagcgc cgcctccgcc tcgtctcact caactcgctg ctcacgtccg aggcgcggcg
     2641 cgaccgcccc aaccccgcgc gcatggccgc actcagggcc gacgtcgaga aggctcggct
     2701 cgagtacgag gacttccaga cgaggctgta cgtggcgcac cccgagttgc gagtccaccg
     2761 cggcgaagcc ccgatcatca aaccggaaga gttaacggcc ctgctgccgg acgcctccac
     2821 ggcgctgctc gaatacgtcg tcgccgccga cgagacctac ctcttcgccg tcacgcgcgc
     2881 ggccggaaag tcggaagccg aactcgtcgt gtacacgatg ccggtcggtc gcgaggagct
     2941 tgcccggcag gccggggcct tccgccgcca gttggccggg cgcgacctcg gcttccgcgc
     3001 cccggcgcgc aagctttacg acctactgct agggccggcc cgggagcagc tccgcggcaa
     3061 gaccagcctc gtcatcgccc cggacaacgt cctctgggag ctgccgttcc aggcgctgct
     3121 gactgacgcg aacaggttcc tgctcgagga ggcggcaatc gcatacgcgc cgtcgctgac
     3181 ggtgctgcgc gagatgacaa ggcggcgcca gagtcaccat tcgggaggcg ccccggccac
     3241 cctgcttgcg ctaggcaacc cactggtcgg gaaagagacg ctgaatcgcg cggcgctgac
     3301 gctgcgcggt gggggacttg acccgctgcc ggaggccgag caggaggtta aggagttggg
     3361 gcggctctac ggctcatcgc ggagtaaggt ctacacgggc gccgaggcgc gcgaggacag
     3421 ggtcaagggt gaggccggcg ggtcgcgtgt gctgcacttc gccgcgcacg caacgctcga
     3481 cgacgccgcg ccgatgtact cgcatctggc gctggccggg ggcggcgccg gcgaggacgg
     3541 gcttctcgag gcgtgggaac tgatgcagct cgacctgcgg gcggacctgg cggtgctctc
     3601 ggcctgcgag acggcacgag gccatgcggc cgcgggcgag ggcgtgatcg ggttctcctg
     3661 ggcgatgttc gtcgcggggg tgccgtcgct ggtcgtcagc cagtggaagg tggaatcggc
     3721 cggcacgcgc gacctgatgg tgaatttcca ccaggcactg ctggcgccgc ccggaggccg
     3781 gaaggggagg gcgggcaagg ccgaggcctt gaggcaggcg gcgctgaggc tgatgaggag
     3841 cccggagacc ggccacccgt tctactgggc ggggttcgtc ctcgtcggag acggcgggtg
     3901 agagggaccc ctttaacggc cggctcggcg taggcagtga ggaggcttga gggggagttc
     3961 gtcggcccgt cgccgctcgc ctccagcggg ggtggctccc ctccggggtt cattggctca
     4021 ctctgggcat gcctctccct ttcatgtaac gttgaatttc atcccttata gcggggttca
     4081 ttcgtatgcg acttccgcca aatgccccat cttacgctcg tttctagcga acacggcggc
     4141 aaactgccag catcaggtcg catccgaatg accaccgctg tagcggagag cttggttcat
     4201 aacctcaagg tcgggagttc aagtctcccc acgctattca atccttccag tgaattagcg
     4261 caggctgatg tccggcgctg agggtgtctc gcgaccgcag catgaattta gtcatttgaa
     4321 ttttcaaatt tcaacagcat gtcaaaatga cgcatacgaa atatatactg ccgtgagtgg
     4381 tagtgacacg ggtgagtcag ttctggccga ctcggcgcgg acgaagtcgc cgccgaactg
     4441 gtcgagcttg tcgagccaga agtggtatcc ctcgaacgtc gggtcgggcc ggccgtcgaa
     4501 gccgacggcg tcggggtcgc gcctgaggta gccgaagtac tgcgccagga cgaacgcgcg
     4561 gttcttctcc gccgccgcga aatcgccgtc ctcacacacg aaccaaactg cctccgcgag
     4621 cgatacatta ccgtggttca gcaactcgac caactcatcg cgctcggcct gcgagagggc
     4681 gcccccggcg tttcggttca aagcgtccac gaaggccccg ttgtccatcc ccggcgggta
     4741 gagcgaggtg aagcggacgg tccggacgaa ctcgttgaag aaggccgccc tgttctgatc
     4801 gagccgccgc cgccagtcgc ccaccccacc tgcacgccgt cgccgatgct ccgcccgccc
     4861 gccaagtact cctgcaacgt cagcgggacc ggcttgttcg gtaggtcgcc gtaagcaacc
     4921 ttgtatgcgc ggtgtgcgag gtagccggtc tcatggtttt cgatggagag gaagaaggcc
     4981 gcactgacgt tgacgcgcgt cgcctcgacg cactgcgcgt ccgcgtccgg gtcgatggtg
     5041 gtgtcgtcgg accagacgaa gctgccgtca tggagggcct gcgcgcgctg gcccgcggcg
     5101 aagctgtagt tgccgtcggc catgttggaa ctcccgccgg gaacggtcgc gtagttgccg
     5161 ctggccctgt tctcggaccc gccgccgacg gtcgcgctgg ccccggtcgc cacgttgaga
     5221 cgcccgccgc cgacggtcgc gcggagaccg ctggcccggt tgtcaattcc gccgccgacg
     5281 gtcgagccgt tatcgccgct ggccgtgttg ccacttccgc cgccgacggt cgcgttgacc
     5341 gcggtgaccg tgttgtttct cccgccgccg accgtcccga aatcttcctg cacactgttc
     5401 acggtggtcg cggcggcgcc gccgccgatt gtcgcacccc ttatgttcgc accgaccgtg
     5461 ttgccggaga aaccgccgat gacgtgcggg ctggtggcgt tctcctgcgt gcgcagcccc
     5521 gtgaagttga ccgccccggc gacgtggagc ctggcctgcg ggttgtccgt ccccaccccg
     5581 acgttgccct cgaagatcgc cccgtcggtc ggcaccgccg tctgcgcgaa gttcgtcccg
     5641 accgccatgt tcccccgcac acccaccgcc gacagcggcg cgaaggcgct cgaattgccc
     5701 gccccgaccg ccagccggtg gttcgcgttg aagatgccca ccccg
//
LOCUS       DBNGOH010000913         3259 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4353121, whole genome shotgun sequence.
ACCESSION   DBNGOH010000913 DBNGOH010000000
VERSION     DBNGOH010000913.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3259)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3259)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3259
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4353121"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..999)
                     /locus_tag="AC159P_17780"
     CDS             complement(<1..999)
                     /locus_tag="AC159P_17780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3697347.1"
                     /translation="MPPTLTPAEGDQPLIAIRELKVHFSLGGGTTWDKITGGSPVRKV
                     VKAVDGVSIDIFPGETLGLVGESGCGKSTLGRALLRLTEPTGGQVLFRGSDLARLSQG
                     QMRKQRRHLQMIFQDPYASLNPRMTVAQIIGEPLETFDLVQSKREGEDRVRELMETVG
                     LSPRFVKRYPHEFSGGQRQRIGIARALAANPDFIVADEPISALDVSIQAQIMNLMERL
                     QQDRELTYLFISHDLRAVRHVSDRVAVMYLGRLVEVADAKVIYDEPLMPYTKALISAV
                     PVPDPKIESSRKRIVLEGDVPSPINPPSGCRFRTRCPYAIDVCAEVEPQLVQIKPNH"
     gene            complement(1124..2215)
                     /locus_tag="AC159P_17785"
     CDS             complement(1124..2215)
                     /locus_tag="AC159P_17785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016523219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3697348.1"
                     /translation="MSSQNGNLLSVRDLRTYFDTEDGVVKAVDGITFNLERGETLGIV
                     GESGSGKSVTNLSIIRLIPSPPGRIVSGEVIFHGQDIMSLPTEEVRKIRGKRISMIFQ
                     DPMTSLNPFMRISKQLMEITQLHLGYTREQAYEHAIKMLEIVGIPEARVRVDSYPHEF
                     SGGMRQRVMIAMALSCEPELLIADEPTTALDVTIQAQILELIKSLKEKTGTSVILITH
                     DLGVVAGMTDHIIIMYAGKVFEQAPTRELFATPGNPYTKGLLRSVPDPAHEQGKALYQ
                     IPGLPPDVAHLPPDQCPFAPRCERAEDVCFRKYPPFVQLNAEHFSLCHFAEEVYAKSR
                     DEREAKAAARAAAVPKALAADSDIGSGAG"
     gene            complement(2340..>3259)
                     /locus_tag="AC159P_17790"
     CDS             complement(2340..>3259)
                     /locus_tag="AC159P_17790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020149775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3697349.1"
                     /translation="EAVKGTSLWKDAWKRLLKNRLAVFGLVVISIIIVVSIIGPPIIR
                     LATGYTYDYIPPDTALNKSFPPFTAPDGSFSWTHPMGTDISGRDILARVLIGGRISLM
                     VGIISTIVSLIIGVTYGATAGYLGGKVDNVMMRIVDVLYSLPYMMIVIVLLAFFGGRS
                     PLGQLFLLFIALGSVSWLTMARIVRGQVLSLKNQEFVLAARATGVTTSRIIFKHLVPN
                     ALGPVIIYSTLTIPSVMLQEAFLSFLGLGVQPPLASWGSLANEGIQNIAIFPWQLIFP
                     GVTMALALFSLNFLGDGLRDALDPQMRKF"
BASE COUNT          557 a          995 c         1147 g          560 t
ORIGIN      
        1 gtgattcggc ttgatctgca ctagctgagg ttcgacctcc gcacagacat cgattgcgta
       61 cggacagcgg gtgcgaaagc ggcagccgct cggaggattg atcggcgaag ggacgtcgcc
      121 ctcgagcacg atccgtttgc gggacgattc aattttcgga tcgggaacgg gaacggccga
      181 aatgagggcc ttcgtgtagg gcatcagcgg ctcgtcgtag ataactttcg cgtcggcgac
      241 ctcgacgagg cgcccgaggt acatgacggc cacccggtcg gagacgtggc gcacggcgcg
      301 caggtcgtgc gagatgaaga ggtaggtgag ctcgcgatcc tgctggagcc gctccatcag
      361 gttcatgatc tgcgcctgaa tggacacgtc gagcgccgag atcggctcgt ccgccacgat
      421 gaagtcgggg ttggcggcca gcgcgcgcgc gatgccgatg cgctggcgct gcccgcccga
      481 gaactcgtgc gggtagcgct tgacgaagcg ggggcttaag ccgacggtct ccatcagctc
      541 gcggacgcgg tcctcgccct cgcgcttgga ctggacgagg tcgaaggttt cgagcggctc
      601 gccgatgatc tgcgcgaccg tcatgcgcgg gttgagggag gcgtacgggt cctggaagat
      661 catttgcagg tggcggcgct gtttacgcat ctgcccctgc gagaggcgcg cgaggtcgct
      721 gccgcggaag agcacctgcc cgccggtcgg ctccgtcagg cgcagcagcg cgcgcccgag
      781 cgtggacttg ccgcagccgg actcgccgac cagccccagc gtctcgccgg ggaagatgtc
      841 gatggagacg ccgtccacgg ccttgacgac tttgcgcacg ggcgagccgc cggtgatctt
      901 gtcccacgtc gtgccgccgc cgagggagaa gtgcaccttc aattcgcgga tggcgatgag
      961 cggctggtcg ccttcggcgg gcgtcagggt cggcggcatc gcgccctttt gcggcgcggt
     1021 ctcggtcggc tgcgaactca tctctgactc accctcccag cgtcttcgaa aagacttcgg
     1081 cttaagttct tccgcaatcg atgacgactc gcgccgccgc gcgtcaaccg gcgccgctgc
     1141 cgatgtcgct gtccgcggcc aacgccttcg ggacggcggc ggcgcgcgcc gccgccttcg
     1201 cctcgcgctc gtcgcgcgac ttcgcgtaga cctcttcggc gaagtggcag agcgagaagt
     1261 gctcggcgtt gagctggacg aacggcgggt acttccggaa gcagacatcc tctgcgcgct
     1321 cgcagcgcgg cgcgaacggg cactggtcgg gcggcaggtg cgccacgtcg ggcggcagtc
     1381 ccggaatctg gtagagcgcc ttgccctgct cgtgcgccgg gtcggggacg gagcggagca
     1441 gccccttcgt gtaggggttg ccgggcgtgg cgaacagctc gcgcgtcggc gcctgctcga
     1501 agaccttgcc cgcgtacatg atgatgatgt ggtcggtcat cccggcgacc acgccgaggt
     1561 cgtgcgtgat gaggatgacg ctggtgcccg tcttctcctt gaggctcttg atgagttcga
     1621 gaatctgcgc ctggatggtc acgtcgagcg cggtcgtcgg ctcgtcggcg atcaggagtt
     1681 ccggctcgca ggagagcgcc atggcgatca tcacgcgctg gcgcatgccg cccgagaact
     1741 cgtgcgggta gctgtccacg cgcacgcgcg cctcggggat gccgacgatc tccagcatct
     1801 tgatggcgtg ctcgtacgcc tgctcgcgcg tgtagccgag gtggagctgc gtgatctcca
     1861 tcaactgctt cgagatgcgc atgaacgggt tgagcgaggt catcgggtcc tggaagatca
     1921 tgctgatgcg cttgccgcga atcttgcgca cctcctcggt cggcaaagac atgatgtcct
     1981 gcccgtggaa gatgacctcg cccgacacga tgcgtccggg cggcgacgga atcaggcgga
     2041 tgatcgagag gttcgtgacg gacttgcccg agccggactc gcccacgatc cccagcgtct
     2101 cgccccgttc gaggttgaac gtgatgccgt ccaccgcctt gacgacgccg tcctccgtgt
     2161 cgaagtacgt ccggaggtct ctgaccgaaa gaaggttgcc gttctggctg ctcataatat
     2221 tcgtaagccg tgaaccggaa accgtgaacc gtggccgagc tgtttctcaa cccggaaggt
     2281 gaatcgcacg tcggactgcg gcagacgatt tacggttcac ggtttccggt tcacggcttt
     2341 cagaatttcc gcatctgcgg gtcgagcgcg tcgcgcaggc cgtcgccgag gaagttcagc
     2401 gagaagagcg cgagcgccat cgtgacgccg gggaagatca gttgccacgg gaagatggcg
     2461 atgttctgga tgccctcgtt ggcgaggctg ccccacgagg cgagcggcgg ctgaacgccc
     2521 aagccgagga acgagaggaa cgcctcctgc aacatcacgg aggggatggt cagcgtcgag
     2581 tagatgatga cggggccgag cgcgttcggg acgagatgct tgaagatgat ccggctggtc
     2641 gtcacgccgg tcgcgcgcgc ggccaggacg aactcctggt tcttgagcga gagcacctgc
     2701 ccgcgcacga tgcgcgccat cgtcagccac gagaccgagc cgagcgcgat gaagagcagg
     2761 aagagctgcc cgagcggcga gcgtccgccg aagaaggcca agagcacgat gacgatcatc
     2821 atgtacggca gcgagtagag cacgtccacg atcctcatca tgacgttgtc caccttgccg
     2881 ccgaggtagc cggcggtcgc gccgtaggtg acgccgatga tgagcgagac gatggtcgag
     2941 atgatgccca ccatcagcga gatgcgcccg ccgatgagca cgcgcgcgag gatgtcgcgc
     3001 ccagagatgt cggtgcccat cgggtgcgtc cacgagaacg agccgtcggg cgcggtgaag
     3061 ggcgggaagg acttgttgag cgccgtgtcg ggcgggatgt agtcgtaggt gtaaccggtc
     3121 gccagacgga tgatcggcgg gccgatgatc gagacgacga tgatgatgga gatgaccacc
     3181 agcccgaaga ccgcgagccg gttcttcagc aaccgcttcc acgcgtcctt ccagagcgaa
     3241 gtgcccttga cggcctcgg
//
LOCUS       DBNGOH010000914         1856 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3254741, whole genome shotgun sequence.
ACCESSION   DBNGOH010000914 DBNGOH010000000
VERSION     DBNGOH010000914.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1856)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1856)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1856
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3254741"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(105..>1856)
                     /locus_tag="AC159P_17795"
     CDS             complement(105..>1856)
                     /locus_tag="AC159P_17795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697350.1"
                     /translation="DELKRPAGDPRARARLRGLLAAAARRDFEAARPLVRFHEILLFL
                     RAYPQSAALLRQTERLLASFSRRVEKLYANEAADLVAFEEAESSGIAGTSFSAVFSYD
                     IARWLAARDPSRAAIEWGGNEDKAQLAATLPLLVPFLDENAYVDIYFSREDWLRAARG
                     RTRQITWLARAFGRWELPEKAKAVVYDSLKLAVRWQLGDTAATRTRMKRPVREVFYHD
                     APLVSRRDVQLARELSDSAPLPVEKLSPAEGRRLLDTGRETMSVRYRELYGFTHGDPR
                     SVRCAEVGRGVEIFLWGVPAARRFPTIAYHSMLIFKNGVPCGYAEGLTLFERTEVGLN
                     LFYTFREGESAWVYARLLRLMRQLLGARVFSVEPYQLGFHNAEGIQSGAFWFYRKLGF
                     RPVLPPLATLVEREERKLAERPGYRSPAHVLRQLATGHMLYEAHASSTDRHAPSSSKE
                     ARTSSTVEQATTTSSGEWDNFHLRRLGLALQRRIAARHDGDPERARRTSAKRLERALG
                     ADTARWNEAERRAFRDLSLVLSLIPNLSRWTPDEQRALLQLVRAKASADETRYLRLLQ
                     QHPRLRREIIRMGSEQE"
BASE COUNT          281 a          556 c          718 g          301 t
ORIGIN      
        1 aagcatcttc gttcgtcaaa cagtacgagg aggatgcttg aggctttcga cctcaagcat
       61 cctcgccatc ctgtcttgac tgcctaccgc ctactgccta ctgcctactc ctgctcactg
      121 cccattctta tgatttcgcg gcggaggcgc gggtgttgtt ggagtagacg gaggtatcgg
      181 gtctcgtccg cggaggcctt ggcgcggacg agttggagga gggcgcgctg ttcgtcgggc
      241 gtccatcgtg agaggttcgg gatgagcgag aggacgagcg agaggtctcg gaaggcgcgg
      301 cgctcggcct cgttccagcg ggcggtgtcg gcgccgaggg cgcgctcgag ccgtttcgcg
      361 gaggtgcggc gcgcgcgttc ggggtcgccg tcgtgacgcg cggcgatgcg ccgttggagg
      421 gcgaggccga ggcggcgcag gtgaaagttg tcccactcgc cggacgatgt agtggtcgct
      481 tgctcgacgg ttgacgaagt gcgtgcttct ttcgatgaag agggcgcgtg tcggtctgtc
      541 gatgaggcgt gtgcttcgta gagcatgtgg ccggtggcga gttgccggag cacatgtgcg
      601 ggggagcggt agccggggcg ttcggcgagc ttgcgttctt cgcgctcgac gagtgtggcc
      661 agaggcggca gcacggggcg gaagccgagc ttgcggtaga accagaaggc gccggactgg
      721 atgccttcgg cgttgtggaa gccgagctgg tacggctcga ccgagaagac gcgcgcgccg
      781 aggagctgcc gcatgaggcg cagcagtcgc gcgtagaccc acgcggactc gccctcgcgg
      841 aaggtgtaga agaggttgag gccgacctcc gtgcgctcga agagcgtgag tccttcggcg
      901 tagccgcagg gcacgccgtt cttgaagatg agcatcgagt ggtaggcgat cgtcgggaag
      961 cgccgcgcgg cgggcacgcc ccacagaaag atttcgacgc cgcggccaac ctccgcgcac
     1021 cgcacgctgc gcgggtcgcc gtgggtgaag ccgtacagct cgcggtagcg caccgacatc
     1081 gtctcgcgcc ccgtgtcgag gagcctgcgg ccttcggcgg gcgagagctt ttcgaccggg
     1141 agcggcgcgg agtccgacag ctcgcgcgca agctgtacgt cgcggcgcga gacgagcggc
     1201 gcgtcgtggt agaagacctc gcgcacgggc cgcttcatcc gcgtgcgcgt cgccgccgtg
     1261 tcgccgagct gccagcgcac ggcgagcttc agagagtcgt agacgacggc cttcgccttc
     1321 tccggcaact cccagcgccc gaaggcgcgg gcgagccaag tgatctggcg cgtgcgaccg
     1381 cgcgccgcgc gcagccagtc ttcgcgcgag aagtagatgt ccacgtaggc gttctcgtcc
     1441 aagaacggca cgaggagcgg gagggtcgcc gcgagctgcg ccttgtcttc gttgccgccc
     1501 cactcgattg cggcgcgcga aggatcgcgc gcggcgagcc agcgcgcgat gtcgtagctg
     1561 aagacggcgg agaacgaagt gccggcgatg ccggacgact cggcctcctc gaaggcgacg
     1621 aggtctgcgg cttcgttcgc gtagagtttt tcgacgcggc ggctgaagga agcgagaagc
     1681 cgctcggtct gtcgcagcag cgcggcgctc tgcgggtagg cgcgcaggaa gaggagtatt
     1741 tcgtggaagc ggacgagcgg gcgggccgct tcgaagtcgc gccgcgcggc tgcggcgagc
     1801 aggccgcgca ggcgcgcgcg ggcgcgcggg tctccggcgg ggcgcttcaa ctcgtc
//
LOCUS       DBNGOH010000915         4357 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4536924, whole genome shotgun sequence.
ACCESSION   DBNGOH010000915 DBNGOH010000000
VERSION     DBNGOH010000915.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4357)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4357)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4357
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4536924"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            114..569
                     /locus_tag="AC159P_17800"
     CDS             114..569
                     /locus_tag="AC159P_17800"
                     /inference="COORDINATES: protein motif:HMM:NF017015.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HIR3697351.1"
                     /translation="MKKSELLERSRGAHGALVSALEGLTEETAARVGVNAQWSVKDVL
                     AHIAAWEAEGVRRFARAGEGGGAAKLDKEAIDSFNAAAVEERRSKSFGELREEYEEAY
                     RGLTGALEALPDELDESSREYRFAEIVAVIHPTHHAAQIEEWKRKLREP"
     gene            653..2056
                     /gene="lpdA"
                     /locus_tag="AC159P_17805"
     CDS             653..2056
                     /gene="lpdA"
                     /locus_tag="AC159P_17805"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     (NADH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HIR3697352.1"
                     /translation="MSTEQFDVTIIGSGPGGYVAAIRGAQLGLKVALVEKDKRLGGTC
                     TLRGCIPTKQLLMSAHVYEQTQHAAEFGVQASEIKLAFADVQKRKDKVVTKNSKGIEF
                     LMKKNRITVFKGTGRLALPGKVEVTGEGGEKQTIDTKNIIIATGSVVRPIPGFETDGE
                     RVVNSDHILELKQVPKSLIVLGSGAVGTEFASVYSRFGSETTLVELLPRILPLEDEEV
                     SKQAEQSFRKRGIKSLVGTKLEKVERTDAGVKVTGKDAKGGDVTLEAEMLLVAVGRMP
                     YLEGLGLEGTKIKVERGNVQVDEFCRTGEPNVYAIGDVIATPWLAHLASKEGIMVVEQ
                     IAGHKTEPINLRQVPSCTYCDPEVASVGLTEAKARELGFEVKVGKFPFSASGKARILG
                     EEEGFVKVVADKKYDEILGVHIIGPHATEIIHEAIVAMRLESTADELGRTIHAHPTVS
                     ESVMEAAEGIHGLTIHF"
     gene            2181..3392
                     /locus_tag="AC159P_17810"
     CDS             2181..3392
                     /locus_tag="AC159P_17810"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HIR3697353.1"
                     /translation="MKTTKTPARTEGKPARGKTKEAGASAAGNGTAGGGGGPFITIEP
                     RATEAAIADPLNEQGELICTSETSLDEEQLFELYRYLQLTRLVEEKLVNLYRQTKVVG
                     GVFRSLGQEATAVGTCYALETQDFIAPLIRDLGAVLVKGIRPREVFAQYMAKAWGPSE
                     GRDLNIHFGDLARGFIGPISHLGDMIPVMTGIALGARMGRRDLVAVAYIGDGGMSTGA
                     FHEGLNFAAVQRLPVVIVAEHNWYAYSTPTRLQTAVENLADKAAGYGIPGYIVDGNDV
                     VACYEVMKRAADFARGGGGAVLIEAKTYRRKGHAEHDDQRYVSASELEEWEKRDPLDR
                     YHRHLVSKGVAGEEKLEEIVADVKREIEEDAAWAESSPMPEAEKAAYNVFDNAVVPPA
                     FRPPVFGPNSE"
     gene            3400..3960
                     /locus_tag="AC159P_17815"
     CDS             3400..3960
                     /locus_tag="AC159P_17815"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007980565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HIR3697354.1"
                     /translation="MRAAFVIFEGMTALDFVGAYDPLTRLGTMKLREDFAWDVCAFTE
                     EVRDNTGLRFAPTRVGQSLGAYDLLVVPGGYAARELTQDAAFLGWLRTGAACDLKASV
                     CTGSLLLGAAGFLEGKRATTHPSALEELKRFCRVSEERIVDEGDVVTAGGVTAAIDLG
                     LHLCERLAGAGAREKIRRQMDYHPVQ"
     gene            4090..>4357
                     /locus_tag="AC159P_17820"
     CDS             4090..>4357
                     /locus_tag="AC159P_17820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pepsin/retropepsin-like aspartic protease family
                     protein"
                     /protein_id="HIR3697355.1"
                     /translation="MGLVYADIELIRGDDLALLRHGVIAEDKVRRMKVSMLVDSGSYM
                     LVINDHIKEQLDLPVLDTQTARLADESEITVDVVGPVEIRFENRA"
BASE COUNT          827 a         1349 c         1509 g          672 t
ORIGIN      
        1 tgcggatttg aaaaagcttc cacacgaatt gtcgagtccg tgcggcgcgg ggagaggacg
       61 aatccgacgc gcgcggcggg cgggtgcgag cgcctcgcgg cggaggtctc gggatgaaaa
      121 agtcggagct gcttgagcgc tcgcgcggcg cgcacggcgc gctcgtgagc gcgctcgaag
      181 ggctgacgga agagacggcg gcgcgcgtcg gcgtcaacgc gcagtggtcc gtgaaggacg
      241 tgctggcgca catcgccgcg tgggaggcag agggcgtgcg ccgcttcgcg cgggcgggcg
      301 agggcggcgg cgcggcgaag ctcgacaagg aggccatcga cagcttcaac gccgcggcgg
      361 tcgaagagcg gcggtcgaaa agtttcggcg agctgcgcga ggagtacgaa gaggcgtacc
      421 gcggcctcac gggggcgctc gaagcgctgc ccgacgagct ggacgagtcg tcgcgcgaat
      481 accgcttcgc cgaaatcgtc gccgtcatcc accccacgca ccacgcggcg cagatcgaag
      541 agtggaagag aaaactccgt gaaccgtaaa ccgtcaaccg tgacaggtaa gaaccggttt
      601 tctactcgtc acggttgacg gtttacggtt cacggccttt gaggagaaag cattgtctac
      661 cgaacaattt gacgtgacga tcatcgggtc ggggccgggc ggttacgtgg cggcgatccg
      721 cggggcgcag ttggggttga aggtggcgct ggtcgagaag gacaagcggc tgggcggcac
      781 gtgcacgctg cgcgggtgca tcccgaccaa gcagctcctg atgtcggcgc acgtctacga
      841 gcagacgcag cacgccgccg agttcggcgt gcaggcttcc gagatcaagc tcgccttcgc
      901 cgatgtgcag aagcgcaagg acaaggtggt gacgaagaac tcgaagggca tcgagttcct
      961 gatgaagaag aaccggatca cggtcttcaa gggcacgggg cggctggcgc tgcccggcaa
     1021 ggtcgaggtg acgggcgagg gcggagagaa gcagaccatc gacacgaaga acatcatcat
     1081 cgccaccggc tcggtcgtgc ggcccatccc cggcttcgag acggacggcg agcgcgtcgt
     1141 caacagcgac cacatcctcg aacttaaaca agtgccgaag tcgctcatcg tgttgggttc
     1201 gggcgcggtc ggcaccgagt tcgcgtccgt ctactcgcgc ttcggctcgg agacgacgct
     1261 cgtcgagctg ctgccgcgca tcctgccgct cgaagacgag gaggtctcga agcaggccga
     1321 gcagtctttc cgcaagcgcg gcatcaagtc gctcgtgggg acgaagctcg aaaaggtcga
     1381 gcgcacggac gcgggcgtga aggtgacggg caaggacgcg aagggcggcg acgtgacgct
     1441 cgaagcagag atgctgctgg tcgccgtcgg gcgcatgccc tacctcgaag gcttggggct
     1501 ggaaggcacg aagatcaagg tcgagcgcgg caacgtgcag gtggacgagt tctgccgcac
     1561 gggcgagccg aacgtctacg ccatcggcga cgtgatcgcc acgccgtggc tcgcgcacct
     1621 cgcgtcgaaa gagggcatca tggtcgtcga gcagatcgcc ggccacaaga ccgagccgat
     1681 taatttgcgc caagtccctt cgtgcaccta ctgcgacccg gaggtggcgt cggtcggact
     1741 caccgaagcg aaggcgcgcg agctggggtt cgaagtgaag gtcgggaagt tccccttctc
     1801 ggcctcgggc aaggcgcgca tcctcggcga ggaggagggc ttcgtcaagg tcgtcgccga
     1861 caagaagtac gacgaaatcc tcggcgtcca catcatcggc ccgcacgcca cggagatcat
     1921 ccacgaagcc atcgtcgcca tgcgtctgga atcgacggcg gacgagctgg gccgcaccat
     1981 ccacgcccac ccgaccgtct ccgagtccgt catggaagcc gccgagggca tccacggcct
     2041 gacgattcac ttttaagagc cgtgaaccgt gaaccgtgaa ccgtgatgag tcggagggcg
     2101 gttttcgatc tccacggttc agggtttacg acttgcggcc ttctcctgtc acggttcacg
     2161 gtttacggtt cacggctttt atgaagacga caaagacacc tgcgcgcacg gaaggcaagc
     2221 ctgcgcgcgg caaaacgaaa gaggcgggcg cgtccgccgc ggggaacggg acggcgggcg
     2281 gcggcggcgg ccccttcatc accatcgagc cgcgcgcgac cgaggcggcg attgcagacc
     2341 ctctgaacga gcagggcgaa ctcatctgca cgagcgagac gagtctggac gaagagcagt
     2401 tgttcgaact ctaccgttat ctgcaactga cgcggctcgt cgaagagaag ctggtcaatc
     2461 tctaccggca gacgaaggtc gtgggcggcg tgttccgctc gctcggtcag gaggcgaccg
     2521 ccgtcggcac gtgctacgcg ctcgagacgc aagacttcat cgcgcctttg atccgcgacc
     2581 tcggcgcggt gctcgtcaag ggcatccgcc cgcgcgaggt cttcgcgcag tacatggcca
     2641 aggcgtgggg gccttccgaa gggcgcgacc tcaacatcca cttcggcgac ctcgcgcgcg
     2701 gcttcatcgg ccccatctcg cacctcggcg acatgatccc cgtcatgacc ggcatcgcgc
     2761 tcggcgcgcg catgggccgc agagacctcg tcgccgtggc ctacatcggc gacggcggga
     2821 tgagcaccgg cgcctttcac gaggggctga acttcgccgc cgtgcagcgc ctgcccgtcg
     2881 tcatcgttgc cgagcacaac tggtacgcct actcgacgcc gacgcgcttg cagaccgccg
     2941 tcgagaacct cgccgacaag gccgccggct acggcatccc cggctacatc gtggacggca
     3001 acgacgtggt cgcctgttac gaggtgatga agcgcgccgc ggacttcgcg cgcggcgggg
     3061 gcggtgcggt tttgatcgag gcgaagacct accgccgcaa aggccacgcc gagcacgacg
     3121 accagcgcta cgtctcggcg agcgaactcg aagagtggga gaagcgcgac ccccttgacc
     3181 gctaccaccg ccacctcgtc tcgaagggcg tggccggcga agagaagctc gaagagatcg
     3241 tcgccgacgt gaagcgcgag atcgaagagg acgcggcgtg ggccgagtcc tcgccgatgc
     3301 ccgaggcgga gaaggccgcc tacaacgtct tcgacaacgc ggtcgtgccg cccgccttcc
     3361 gcccgccggt cttcggcccg aactcggagt gacctgactt tgcgcgcggc cttcgtcatc
     3421 ttcgaaggga tgacggcgct cgacttcgtc ggcgcctacg accccctgac gcgcctcggc
     3481 acgatgaaac tgcgcgaaga cttcgcgtgg gacgtgtgcg ccttcaccga ggaagtgcgc
     3541 gacaacaccg gcttgcgctt cgcgccgacg cgcgtcgggc aatcgctcgg cgcttatgat
     3601 ttgctcgtcg tgcccggcgg ctacgccgcg agggagctga cgcaagacgc cgccttcctc
     3661 ggctggctgc gcaccggcgc cgcgtgcgac ttgaaagcct ccgtctgcac cggctcgctc
     3721 ctgctcggcg ccgccgggtt tttggagggc aagcgcgcga ccacgcaccc gtcggcgctg
     3781 gaagagctga aaaggttctg ccgcgtctcg gaagagcgaa tcgtagacga gggcgacgtg
     3841 gtcacggcgg gcggcgtcac cgccgccatc gacctcggcc tccacctctg cgaaagactc
     3901 gcgggcgccg gggcgcgcga gaagattcgg aggcagatgg attaccatcc tgtgcaatga
     3961 aagctcggcc tacgagatgc gacgcgcgtc tcgcgtgaaa gtgtttgaga ggtgagtatg
     4021 attttacccg ctggtcaggg ccgcacaaaa ttcccgcctt tggagaccgt ttcttacggg
     4081 gagaagaaga tgggattagt gtacgcggac atcgagctaa tcaggggcga cgacctcgcg
     4141 ctgctgcgcc acggcgtcat cgccgaggac aaggtcaggc ggatgaaggt gagcatgctc
     4201 gttgacagcg ggtcgtacat gctcgtgatt aacgaccaca tcaaggagca actcgacctg
     4261 ccagtgctgg atactcagac ggcgcggctg gcggacgaga gcgagattac ggtcgatgta
     4321 gtagggccgg tcgaaatccg ttttgagaac cgcgcag
//
LOCUS       DBNGOH010000916         2214 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4537383, whole genome shotgun sequence.
ACCESSION   DBNGOH010000916 DBNGOH010000000
VERSION     DBNGOH010000916.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2214)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2214)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2214
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4537383"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2105)
                     /locus_tag="AC159P_17825"
     CDS             complement(<1..2105)
                     /locus_tag="AC159P_17825"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019922292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 protein"
                     /protein_id="HIR3697356.1"
                     /translation="MNALKRLLVRSCLALLCASTVVFAAAPPTSRRAKYNFNPGWKFF
                     VGDPGGAEQPGFDDSAWKAVTLPRAWTEDEAFRKDIKDLSNGVAWYRKRFRLPADAAG
                     KKVFLEFEGIRHGGEFYLNGKHIGRHENGVMAFGLDITGHVLPAPQENVIAARIDSSW
                     NYKEKATGSGYQWSDKNFYANYGGLNKNVYLHLADKLHQTLPLYSNLSTTGVYVYARE
                     FDIRGRSATVTAESQVRNEHATAKTFRYRAVVEDMGGRAVKTLDGGTHTLAPGETKTV
                     SASARVGGLNFWSWGYGYLYNVRTILTGVDGRAVDEVGTRTGFRKTEFGGGLVRLNDR
                     VIQLKGYAQRTTNEWPAIGVSVPAWVSDFSNRLMVESGANLVRWMHMTPWKQDVESCD
                     RVGLLQAAPAGDAERDVEGRRWEHRVELMRDAIIYLRNNPSVVFWESGNESISEEHMR
                     QMKALRDRYDPHGGRAAGSREMLDSRVAEYGGEMLYINKSARLPVWAMEYSRDEGLRK
                     YWDEHSPPFHKEGEGPLYRGQDASVYNRNQDSHAVENVVRWYDYWRERPGTGTRVNSG
                     GVNIVFSDSNTHYRGAENYRRSGEVDAVRLPKDGFYAHQVMWDGWVDVEEARTHIVGH
                     WNYAPGTKKNIYVISSAERVELFVNGKSLGAGEQSSRFLFTFKNVEWQPGEVKAVGYD
                     AGRRKINEASKKTAGPPAA"
BASE COUNT          319 a          776 c          702 g          417 t
ORIGIN      
        1 atcgcggcgg gcggccccgc ggtcttcttc gacgcctcgt tgatcttcct gcgccccgcg
       61 tcatagccga cggccttcac ctcgcccggc tgccactcga cgttcttgaa ggtgaacaga
      121 aagcggctcg actgctcgcc cgcgccgagc gacttgccgt tgacgaacag ctccacacgt
      181 tcggcgcttg agatgacgta gatgttcttc ttcgtccccg gcgcgtagtt ccagtggccg
      241 acgatgtgcg tgcgcgcctc ctccacgtca acccagccgt cccacatcac ctgatgcgcg
      301 tagaagccgt ccttcggcag ccgcacggcg tccacctcgc cgctgcgccg gtagttctcg
      361 gccccccggt agtgtgtgtt cgagtcggag aagacgatgt tgacgccgcc ggagttgacg
      421 cgcgtgcccg tccccggacg ctcgcgccag tagtcgtacc agcgcaccac gttctcgacg
      481 gcgtgcgagt cctggttgcg gttgtagacg ctcgcgtcct gcccacggta gagcggcccc
      541 tcgccctcct tgtggaaagg cggcgagtgc tcgtcccagt atttcctcaa accctcgtcg
      601 cgcgagtact ccatcgccca cacgggcagc cgcgcgctct tgttgatgta gagcatctcg
      661 ccgccgtact cggcgacgcg gctgtcgagc atctcgcggc tccccgcggc gcgcccgccg
      721 tgcgggtcgt accggtcgcg caaggctttc atctgccgca tgtgctcttc gctgatgctc
      781 tcgttgcccg actcccagaa gacgacgctc gggttgttgc gcagatagat gatggcgtcg
      841 cgcatcagct cgacgcggtg ctcccagcgg cggccctcca cgtccctttc ggcgtcgccc
      901 gcgggcgcgg cctgcaacag ccccacgcgg tcgcacgact ccacgtcctg cttccacggc
      961 gtcatgtgca tccagcggac gaggttggcg cccgactcga ccatcagacg gttgctgaag
     1021 tcgctgaccc acgccggcac cgacaccccg atggccggcc attcgttcgt ggtgcgctgg
     1081 gcgtagccct tgagctggat gacgcggtcg ttgagacgca ccagcccgcc gccgaactcg
     1141 gtcttgcgga agcccgtccg cgtgccgact tcgtcaaccg cgcgcccgtc aacccctgtg
     1201 aggatcgtgc ggacgttgta gaggtagccg tagccccagc tccagaagtt gaggccgccg
     1261 actctcgctg acgcgctcac cgtcttcgtc tcgcccggcg cgagcgtgtg cgtgccgccg
     1321 tcgagcgtct tgaccgcgcg cccgcccatg tcctcgacca cggcccggta gcggaacgtc
     1381 ttcgccgtcg cgtgctcgtt cctcacctgc gactcggcgg tgacggtcgc gctccggccc
     1441 ctgatgtcga actcgcgcgc gtagacgtag acgcccgtcg tcgagaggtt cgagtagagc
     1501 gggagcgtct ggtggagctt gtccgcgagg tgcaggtaga cgttcttgtt gaggccgccg
     1561 tagttggcgt aaaaattctt gtcgctccac tggtagccgg agccggtcgc cttctctttg
     1621 tagttccaag aactgtcgat ccgcgccgcg atcacgttct cctgcggcgc gggcagcacg
     1681 tgcccggtga tgtcgaggcc gaaggccatg acgccgttct cgtggcgccc gatgtgtttg
     1741 ccgttgaggt agaactcgcc gccgtgccgg atgccctcga actcgaggaa gaccttcttg
     1801 ccggcggcgt ccgccgggag ccggaagcgc ttgcgatacc acgcgacgcc gttcgagagg
     1861 tctttgatgt ccttccggaa cgcctcgtcc tcggtccacg cgcgcggcag cgtcacggcc
     1921 ttccacgcag agtcgtcgaa gccgggctgt tcggcgcccc ccgggtcgcc gacgaagaac
     1981 ttccagccgg ggttgaagtt gtacttcgcc cgccggctcg tgggcggggc ggctgcgaag
     2041 accacggtcg aggcgcacag caacgccagg caacttcgaa cgagtagacg tttcagcgca
     2101 ttcatataaa gcctacgaat gtgttcgttg acgagcaagc atcacaacca tcggcaagcc
     2161 ccgcgccgcg tggcggcagc cggcgcggac gcggcctgtc gtgcttgcaa aacg
//
LOCUS       DBNGOH010000917         2571 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1790010, whole genome shotgun sequence.
ACCESSION   DBNGOH010000917 DBNGOH010000000
VERSION     DBNGOH010000917.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2571)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2571)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2571
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1790010"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1073
                     /locus_tag="AC159P_17830"
     CDS             <1..1073
                     /locus_tag="AC159P_17830"
                     /inference="COORDINATES: protein motif:HMM:NF013491.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HIR3697357.1"
                     /translation="TLPDKYTIKRADLSQIDEYQKRLAARQDLMRPRADLSEKRLLSL
                     HQQRARMSPAYGAKSANLGEVSAARLRGITVPAGFGIPVFYYDQFVRENKLDEAIAEM
                     MNDQRFVHDPAYRRERLAEMRARIQRGAVNDRLRAEIARRVRAEFAGKGLFVRSSSNV
                     EDLPNFNGAGLYDTVPNVKEEEKLVEALKTVWASLWNFQAYEARERANVDHSQSFMGV
                     LVQEGVNAESAGVLITADPFNADNRDAIYISAKRGLGIKVVEGKRIPEQLLYLPRANS
                     VKVLTRSEEESLLTFDETGGVKELPITGERAVLTDEVVRRLARAASLIKNVFGGKEQD
                     IEWVYTRGEIYIVQSRPYVQGS"
     gene            1079..1591
                     /locus_tag="AC159P_17835"
     CDS             1079..1591
                     /locus_tag="AC159P_17835"
                     /inference="COORDINATES: protein motif:HMM:NF012725.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3697358.1"
                     /translation="MTNIVRGQARSVCFVAAVAGALLFASAACREGGTNESAQTNAGA
                     ASNNSAAASNTAAGGDPSKLDAEIERLEKQAEKNPGNAETLAELARAYVRRGNAYRDA
                     QRLREAMDDYRRAQRNDPDNEEAQRNAAEIAPQVEGTPTGEYGEPAPLPISPNVADDE
                     TKPTPTPKKP"
     gene            1708..2430
                     /gene="hisA"
                     /locus_tag="AC159P_17840"
     CDS             1708..2430
                     /gene="hisA"
                     /locus_tag="AC159P_17840"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HIR3697359.1"
                     /translation="MLIIPAIDLRDGRCVRLTQGRRAEVKVYDGDPVEIARGFEAAGA
                     RMLHVVDLDGAFADSNATNRKVVRRIIRAVEIPVQFGGGLRRIEDVQQLVEYGAAQVV
                     IGTLAAESPEQLERFVQLFGFRISVGIDARRGAVLTHGWEREGELTAVELARRVADVG
                     VDRIVYTDVARDGMLGGVNLEQTCAIARESGLRVTASGGVSSLADLRELKSIQPCGVD
                     SVIVGKALYEGRFTLKDALAAV"
BASE COUNT          456 a          850 c          897 g          368 t
ORIGIN      
        1 agacgctgcc cgacaagtac acgatcaagc gcgcagacct cagccagatc gacgaatacc
       61 agaagcgtct ggccgcgcgg caggacttga tgcgcccgcg cgcagacctc tccgagaagc
      121 ggttgctctc gctccaccag cagcgcgcgc ggatgtcgcc ggcctacggc gcgaagtccg
      181 ccaacctcgg cgaggtgagc gccgcgcgcc tgcgcggcat caccgttccc gctggcttcg
      241 gcatccccgt cttctactac gaccagttcg tcagggagaa caagctcgac gaggccatcg
      301 ccgagatgat gaacgaccag cgcttcgtcc acgacccggc ctaccgccgc gagcgtctgg
      361 cggagatgcg cgcgcgaatc cagcggggcg cggtcaacga caggctgcgc gcggagatcg
      421 cgcggcgcgt gcgcgcggag ttcgccggca aggggctgtt cgtccgcagc tcctcgaacg
      481 tcgaagacct gccgaacttc aacggcgccg gactctacga caccgtgccg aacgtgaagg
      541 aagaggagaa gctcgtcgag gcgttgaaga ccgtgtgggc ctcgctctgg aactttcagg
      601 cgtacgaggc gcgcgagcgc gccaacgtcg atcactcgca gtccttcatg ggcgtcctcg
      661 tgcaggaggg cgtcaacgcc gagagcgcgg gcgtgctcat caccgccgac cccttcaacg
      721 cggacaaccg cgacgccatc tacatcagcg ccaagcgcgg cctcggcatc aaggtcgtcg
      781 aaggcaagcg catccccgaa cagctcctct acctgccgcg cgccaactcc gtgaaagtgt
      841 tgacgcgctc cgaggaggag agcctgctca ccttcgacga gacgggcggc gtcaaggagt
      901 tgccgatcac gggcgagcgc gccgtcctga ccgacgaggt ggtgcggagg ctggcgcgcg
      961 ccgcctcgct catcaagaat gtcttcggcg gcaaagagca ggacatcgag tgggtctaca
     1021 cgcgcggcga gatttacatc gtgcagtcgc gcccgtacgt gcaggggagt taaggctgat
     1081 gacaaacatc gtgcggggac aggcccgctc cgtctgcttc gtcgccgccg ttgcgggcgc
     1141 gctgctcttc gcctcggcgg cgtgccgcga gggcggcacg aacgagtcgg cgcagaccaa
     1201 cgcgggcgcg gcctcgaaca attccgcggc ggcgagcaac acggcggcgg gcggcgaccc
     1261 gtcgaagctc gacgccgaga tcgagcgact cgaaaagcag gccgagaaga accccggcaa
     1321 cgccgagacg ctggccgagc tggcgcgcgc ctacgtccgc cgcggcaacg cctaccgcga
     1381 cgcccagcgc ctgcgcgaag cgatggacga ctaccgccgc gcgcagcgca acgaccccga
     1441 caacgaggag gcgcaacgca acgccgccga gatcgccccg caggtcgaag gcacgcccac
     1501 gggcgagtac ggcgagccgg ccccgctccc catctcaccc aacgtcgccg acgacgaaac
     1561 gaagccgacg ccgacgccca aaaagccgtg aaccgtaaac cgtaaaccgt gataagttga
     1621 aatagcgtct ctctcatcaa cgttccggtt cactattcgc gctttcctct tgtcacggtt
     1681 cacggtttac ggttcacggc ttttgccatg ctgattattc ccgcgatcga tctgagggac
     1741 gggcggtgcg tgcggctgac gcaggggcgg cgcgcggagg tgaaggtgta cgacggcgac
     1801 cccgtggaga tcgcgcgggg gttcgaggcg gcgggcgcgc ggatgctgca cgtggtcgat
     1861 ctggacgggg cgttcgcgga ctcgaacgcg acgaaccgga aggtggtgcg gcgcatcatc
     1921 cgcgcggtgg agattccggt gcagttcgga gggggcttgc ggaggatcga ggacgtgcag
     1981 cagctcgtcg agtacggcgc ggcgcaggtc gtcatcggga cgctggcggc ggagtcgccc
     2041 gagcagcttg aaaggttcgt ccagctcttc ggcttccgca tctcggtcgg catcgacgcg
     2101 cggcgcggcg ccgtcctgac gcacggctgg gagcgcgagg gcgagctgac ggcggtcgag
     2161 ctggcgcggc gcgtggccga cgtgggcgtc gaccgcatcg tctacacgga cgtggcgcgc
     2221 gacgggatgc tcggcggcgt caacctcgaa cagacgtgcg cgattgcgcg cgagtccggt
     2281 ctgcgcgtga cggcctcggg cggcgtctcc tcgctcgccg acctgcggga gctgaagagc
     2341 atccagccgt gcggcgtgga cagcgtcatc gtgggcaagg cgctctacga aggtcgcttc
     2401 acactgaagg acgcgctcgc cgccgtctga gcgacgcgag ttcaaagttc aaggctgagc
     2461 ggcacgtcgc cgcgccgcgg cggttgagtt tagcccggcg tttcaacgcc gggtcgatga
     2521 cgtttcatga agccggtgtc gcgtcggcga cacctgaacg gatgccccaa t
//
LOCUS       DBNGOH010000918         8303 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3989221, whole genome shotgun sequence.
ACCESSION   DBNGOH010000918 DBNGOH010000000
VERSION     DBNGOH010000918.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8303)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8303)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8303
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3989221"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..387)
                     /locus_tag="AC159P_17845"
     CDS             complement(<1..387)
                     /locus_tag="AC159P_17845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HIR3697360.1"
                     /translation="MTTTKKKLAGGALAFAAALMLGVVGLAQQDGPRGFGGHGPGGHG
                     RRGPGGPGESRGPRGFDGLGPLARDLNLTDAQKAEVKKITDAFEASTQALHEQLRQAG
                     PGGGGPFEGLKDGAFDEAAVRAAAQAR"
     gene            632..1363
                     /locus_tag="AC159P_17850"
     CDS             632..1363
                     /locus_tag="AC159P_17850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011525257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR3697361.1"
                     /translation="MEHVLIVDDDRELGELVAELLNEEGFDVEVANRSDRGLERALSG
                     EHSLVVLDVMMPGMNGFALLRRLRAEGSQVPVLMLTARGEDLDRIVGLEIGADDYLPK
                     PFNPRELIARIHAILRRLRPRAAEGAGGATAAPSAPARVAVGDVEVDTGTRQVKRGGE
                     PVELTNVEYEILVMLLGSAGRVVKREDLVRTTLGRELSVFDRSLDMHISHLRKKLGHR
                     SGEVERIKTVRGVGYIYTNTVNREP"
     gene            1462..2928
                     /locus_tag="AC159P_17855"
     CDS             1462..2928
                     /locus_tag="AC159P_17855"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR3697362.1"
                     /translation="MSLYLKVFLWFWLATALVIGALAFASYATRGERAMQPPQFVVGI
                     MSAYARHAVETFEREGPAALDAFLGRLEQEANVRGRLFDAGGAELAGRAATEEEQELA
                     ARAARAGSTVSRMSGGRVIDAFPWPGRGGRGFVFVATMSRRPSAPDGQTAPARRPFPP
                     PGPFGFLLGESTAALAARLVAALLTAGVLCYGLARYIVSPVVKLRAVTRQVAGGDLSA
                     RVGPLLGGRSDELGAMGRDFDAMAARIEALLSAQTRLLRDISHELRSPLARLSVALDL
                     ARKRAGPEAAGQLARIEREAGRLNEMIGQLLTLSRWEAGAGGSRRESVDLAALVREVA
                     EDADFEARGQNRAVELVACDACATAGTPALLRSAFENVVRNAVRHTPEGTAVRISLRC
                     ARDGEEDDGDGAGRAVVTVRDEGAGVPAEALTEIFRPFYRVDDARDRESGGTGLGLAI
                     TERAVRLHGGTVTAENVAGGGFVVEIRLPLDAHLLHTP"
     gene            3182..4024
                     /locus_tag="AC159P_17860"
     CDS             3182..4024
                     /locus_tag="AC159P_17860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3697363.1"
                     /translation="MSNLGIEVRNLHKVFRKRKGFLRGRVTETKALNDVSFEVALGET
                     YGLLGPNGSGKSTLIRVLSTLLVPDGGDVRLLGHRIPGEEDLVRQKIGRVSVDAAFYK
                     KLSARENLLYSAFLYGLDKAHAERRALEILEALGLEGEKFTDPLEEMSRGMQQKVAIT
                     RALLVNPPLLLLDEPTTGLDPKSKRDVQTFLEDLRRREGTTILLTSHDMAETERLCAR
                     IGFIAKGRLIAEGTADELKRRSSASSLDDAFIALAGEKLDEENGDKKSAPKEPEAVLT
                     AEAG"
     gene            4024..4848
                     /locus_tag="AC159P_17865"
     CDS             4024..4848
                     /locus_tag="AC159P_17865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013458739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3697364.1"
                     /translation="MEQVLRQTWAFFFRDYHLTRRYMSWVIVFTFYAVVNTATIALIG
                     VALGDRRLTLLLILGALLWNFLSVLFSEIANSITYERWEGTLEYTFMAPVSRLVHLAG
                     VSMFVVLYSVVRTAIIVASLMLFIRFDLSQANLPGLLVVLVVSSAAFIGLGLMAAVLP
                     VISPERGAEATNIFQGILLLVSGVYYPVEVLPAWLQPLSWVSPATYALSASRKLIGIG
                     ATAADPLQSEGAPLSAVVPELLTLALMGAVMIPLGLYVFGRVEMWAKRTGKLKRTG"
     gene            complement(4955..5413)
                     /gene="bcp"
                     /locus_tag="AC159P_17870"
     CDS             complement(4955..5413)
                     /gene="bcp"
                     /locus_tag="AC159P_17870"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011244597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HIR3697365.1"
                     /translation="MIKEGDTAPDFTARDAEGNEVKLSDLRGQRVVLYFYPKDDTPGC
                     TKQACSFRDAHDVYGEQGIKVLGVSLDDEASHQKFASKYDLPFTLLADTDHTISDAYG
                     VYGEKNYMGKTYMGVSRKTFLIDEEGHVKKVFDKVNVEQHADEVLAAFGG"
     gene            complement(5578..6369)
                     /locus_tag="AC159P_17875"
     CDS             complement(5578..6369)
                     /locus_tag="AC159P_17875"
                     /inference="COORDINATES: protein motif:HMM:NF012376.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HIR3697366.1"
                     /translation="MSTFVIGDVHGRREQLRRLVGMLPRDPARDTLVLLGDLIDRGDD
                     VPGVVRDALALVRDPAAGHVVVLRGNHEQMLLDFMDTGAPLWLHPACGGDDTFEQYAG
                     FALTEHLRADCAKARRKITEAVPAEHVEFFRSLPLYHEDDYAIYVHAGLDCKKHPCET
                     EARLLLWTRDSYFYKHYHGKPCVFGHTPTPLLPLLGRLGRHGIYVAHSAIGIDTGYSD
                     ASPLTCLRLPEFTLYQTYADGRADTHQFTSFIPEHLRAFQRNGQH"
     gene            complement(6393..>8303)
                     /locus_tag="AC159P_17880"
     CDS             complement(6393..>8303)
                     /locus_tag="AC159P_17880"
                     /inference="COORDINATES: protein motif:HMM:NF024821.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3697367.1"
                     /translation="DYELAENAFSRALDLDPNLVEARILMVFIYLSRGEKRKARDEVA
                     HLARKAPNEAAVYFVKGALHRLDGEYDRALRAFDRLSRLDPAARVVTSYNRARVMMYQ
                     HRFDDALLELEHGAKIQPDHPLIKTFRAVVLGRKGDVDEATRLLRQVLDEHPTMDGIR
                     PILAQMLVRQGNRDEARAQLNDRVRDAASADHDIAYWLATAYAMLGEADEAFEWLERA
                     INLGNENRTWFESDPNWGELHDDPRFRELMRHIETEAEKSIDQTIAQELSVDRTPADA
                     APSSARTTASAAAYEEYLRGRDAGGRFIYHTLSREDSDEAIRHFRRALELDPNFALAH
                     CALGGAYANRVIKGFGGPEDYERAEQAFEKALALDPKLLEARLHMVFIYLARGEKQKA
                     RAWVELMRAEAPNDVGVQFVSATLARLDGRYDDALEGFSRMLKVNPAERVVVSYNRAR
                     VMMYQSRYDDALAELDLGAQMEPNHPLIKTFRAVVLSRRGDPAAAIKLMREVLERHPG
                     MDGVRPLLAQFLVKTGDVAAARGELTERVLDAAAADHDVAYWLATAYAMLGEADEAFE
                     WLERAITLGNENQPWFESDPNWEPLRDDPRFRELMRRIESAHAGPAKSATKKDESGGE
                     EPKGGTSSAGSQ"
BASE COUNT         1217 a         2705 c         2937 g         1444 t
ORIGIN      
        1 ccgcgcctgc gccgcggcgc gcaccgccgc ctcgtcgaag gcgccgtcct tcagaccctc
       61 gaacggcccg ccgccgccgg ggccggcctg ccgcagttgc tcgtggagcg cctgcgtgct
      121 cgcctcgaag gcgtcggtga tcttcttgac ctcggccttc tgcgcgtcgg tcaggttgag
      181 gtcgcgcgcg agcggcccga ggccgtcgaa gccgcgcggc ccgcgcgact caccggggcc
      241 gccggggccg cgccgaccgt ggccgcccgg cccgtggccg ccgaagccgc gcgggccgtc
      301 ctgctgcgcc agtccgacca cgcccagcat cagtgcggcg gcgaacgcca gtgcgccgcc
      361 cgccagtttc ttcttcgtcg ttgtcatcgt ctcttccctt tcgccaaaat gaatctcgac
      421 ttcaatgaat ctcgacttcg aggagcaccg cgctcttcgc ttcacggcat tagacgacaa
      481 agcgccacgc gcggacgtaa agagtcggtc aaccccgtgt aaagaagtgt cagccgcggg
      541 ccgacacttc tttacggatg gtcgggcaag gcttaagtta gaatcagccg tttcgactgt
      601 ggcgcgccgc gccggggggt gaacgcaggt gatggaacac gtgctgatcg tggacgacga
      661 ccgcgagctg ggcgagctgg tcgccgagct gctgaacgaa gagggcttcg acgtggaggt
      721 ggcgaaccgc agcgaccgcg gactcgaacg cgcgctctcg ggcgagcact cgctcgtcgt
      781 cctcgacgtg atgatgccgg gcatgaacgg cttcgcgctg ctgcggcggc tgcgcgccga
      841 aggctcgcag gtgcccgtgc tgatgctcac cgcccgcggc gaagacctcg accgcatcgt
      901 cggcctcgag atcggggccg acgactatct gcccaagccc ttcaacccgc gcgagctgat
      961 cgcgcgcatc cacgccatcc tccggcgcct ccgtccgcgc gccgccgaag gcgcaggcgg
     1021 cgcgacggct gcgccttcgg cgcccgcgcg cgtcgcggtc ggcgacgtgg aggtggacac
     1081 cggcacgcgg caggtgaagc gcggcggcga gccggtcgag ctgacgaacg tcgagtacga
     1141 aatcctcgtc atgctgctcg ggtcggcggg ccgcgtcgtc aagcgcgaag acctcgtccg
     1201 cacgacgctc gggcgcgagc tgtcggtctt cgaccgcagc ctcgacatgc acatcagcca
     1261 cctccgcaaa aagctcggcc accgctccgg cgaagtcgag cgcatcaaga cggtgcgcgg
     1321 cgtcggctac atctatacga ataccgtgaa ccgtgagccg tgaaccgtga cgagttaaaa
     1381 cagcatgcct cttaacgttc accgttcgat ctccacgtcc tttcacttgt cacggttcac
     1441 ggttcacggc tcacggctct tatgagttta tacctcaaag tctttctctg gttctggctg
     1501 gcgacggcgc tggtcatcgg ggcgctggcg ttcgcgtcgt acgcgacgcg cggcgagcgc
     1561 gcgatgcagc cgccgcagtt cgtcgtgggg atcatgtcgg cctacgcgcg ccacgccgtc
     1621 gagaccttcg agcgcgaagg cccggcggcg ctcgacgcat tcctcgggcg gctggagcag
     1681 gaggcgaacg tccgcgggcg gctgttcgac gcggggggcg cggagctggc ggggcgcgcc
     1741 gcgaccgaag aggagcagga gttggcggcg cgcgccgcgc gtgcgggcag cacggtgtcg
     1801 aggatgtccg gcggcagggt gatagacgcg ttcccgtggc cggggcgcgg ggggcgcggg
     1861 ttcgtgttcg tggcgacgat gtcgcgcagg ccgtcggcgc cggacggcca aacggccccc
     1921 gcgcgcaggc cgttcccgcc gccggggccg ttcggcttcc tgctcggtga aagcacggcg
     1981 gcgctcgccg cgcgcctcgt cgcggcgctc ctcaccgccg gggtcctctg ctacgggctg
     2041 gcccgctaca tcgtctcgcc ggtcgtcaag ctgcgcgccg tgacgcggca ggtggcgggc
     2101 ggcgatcttt cggcccgcgt cggcccgctg ctcggcgggc ggagcgacga gctgggcgcg
     2161 atgggccgcg acttcgacgc gatggccgcg cgcatcgagg cgctgctgag cgcgcagacg
     2221 agactcctgc gcgacatctc gcacgagctg cgctcgccgc tcgcgcgcct ctcggtcgct
     2281 ttagacctcg cgcgcaaacg cgccgggccg gaggcggcgg gccagctcgc gcgcatcgag
     2341 cgcgaggccg ggcggctcaa cgagatgatc ggccaactgc tgacgctctc gcgctgggag
     2401 gcgggcgcgg gcgggtcgcg cagggaatcg gtagacctcg ccgcgctcgt gcgcgaggtg
     2461 gccgaggacg cggacttcga ggcgcgcggc cagaaccgcg ccgtcgagtt ggtcgcgtgc
     2521 gacgcgtgcg cgaccgccgg gacgcccgcc ctgctccgga gcgcgttcga gaacgtcgtc
     2581 cgcaacgccg tgcgccacac gcccgaaggc accgccgtcc ggatttcact ccgctgcgcg
     2641 cgcgacggcg aagaggacga cggcgacggc gcgggccgcg ccgtggtcac cgtccgcgac
     2701 gaaggagcgg gcgtgcccgc cgaggcgctg acggaaatct tccgcccctt ctaccgcgtg
     2761 gacgacgcgc gcgaccgcga gtcgggcggc accggtttgg gactcgccat cacggagcgc
     2821 gcggtgcgtc tgcacggcgg caccgtcacg gcggagaacg tcgcgggcgg cggcttcgtc
     2881 gtcgagattc gtctgccgct cgacgcgcac cttctccaca cgccctaagc cttcacctgc
     2941 tatcagacca aactcgattg aactttcatc actgcccgag cgtttgcgaa attaaggctt
     3001 gctaaaatcg cccgtgcggc ttatgctggc ctcacctgct aagaggacta agatcatgtt
     3061 tatgatgatt cgtcgctggc tcgcctaact ctaccgcaaa cggaaccctc cgtttcttcc
     3121 ttcatttcca acttggaaag cggttatccg accaacttct ggtcgaaagg tgtgtgggtc
     3181 gatgtcgaat ctggggatcg aagtccgcaa tcttcacaaa gtcttccgta aacgcaaagg
     3241 cttcctgcgc ggtcgtgtca ccgagacgaa agcgctgaac gacgtgtctt tcgaggtcgc
     3301 gctcggcgag acctacgggc tgctggggcc gaacgggtcg ggcaagtcca cgctcatccg
     3361 cgtgctctcg accttgctcg tgccggacgg cggagacgtg cgcctgctcg gccaccgcat
     3421 ccccggcgag gaggacctgg tgcggcagaa gatcgggcgc gtgagcgtgg acgcggcgtt
     3481 ctacaagaaa ttgtcggcgc gcgagaacct gctctactcg gccttcctct acggactcga
     3541 caaggcgcac gccgagcgca gggcgctgga gattctggag gctctgggcc tcgaaggcga
     3601 gaagttcacc gacccgctcg aagagatgag ccgcggcatg cagcagaagg tggcgatcac
     3661 gcgcgcgctg ctggtcaacc cgccgctgct gctcttggac gagccgacga cgggcctcga
     3721 cccgaagagc aagcgcgacg tgcagacgtt cctcgaagac ctgcggcggc gcgaggggac
     3781 gaccatcctc ttgacgtcgc acgacatggc cgagaccgag cggctgtgtg cgcgcatcgg
     3841 cttcatcgcc aagggccggc tcatcgccga ggggacggcg gacgagctga agcggcgctc
     3901 gtcggcctcc tcgctcgacg acgccttcat cgcgctcgcg ggcgagaagc tggacgagga
     3961 gaacggcgac aagaagagcg cgccgaaaga gccggaggcc gtgctcacgg cggaggcggg
     4021 gtgatggaac aggttttaag acagacgtgg gccttctttt tccgcgacta ccacctgacg
     4081 cggcggtaca tgtcgtgggt catcgtgttc accttctacg cggtcgtgaa cacggccacc
     4141 atcgcgctca tcggcgtggc gctcggcgac cgccgtttga cgctgctgct gattttgggc
     4201 gcgctgctgt ggaacttcct ctcggtgctc ttctcggaga tcgccaactc gatcacctac
     4261 gagcgctggg aggggacgct cgaatacacc ttcatggcac cggtctcaag gctggtgcat
     4321 ctggcgggcg tgtcgatgtt cgtcgtgctc tacagcgtcg tgcgcacggc gatcatcgtc
     4381 gcgtcgctga tgctcttcat ccgcttcgac ctctcgcagg cgaacctgcc ggggctgctc
     4441 gtggtgctgg tggtgagcag cgcggccttc atcgggctgg gcctgatggc ggcggtgctg
     4501 cccgtcatca gccccgagcg cggggccgag gcgaccaaca tcttccaggg catcctgctg
     4561 ctggtgtcgg gcgtgtatta cccggtcgag gtgctgcccg cgtggctgca accgctctcg
     4621 tgggtctcgc ccgcgaccta cgcgctgtcg gcgagccgca agctgatcgg catcggcgcc
     4681 accgccgccg accctttgca gtccgagggc gcgccgctct ctgcggtcgt gcccgagctg
     4741 ctgacgctcg cgctgatggg cgccgtcatg atcccgctcg gcctctacgt cttcgggcgc
     4801 gtcgagatgt gggcgaagcg caccggcaag ctcaagcgga caggataaga cagggggaaa
     4861 aggttaaaag gggaagaggc gaaaagggag atgaagcaac tcgcttgtct tcccttttcg
     4921 ccgcttcccc ttttaacctt ttcccccttg atctttaccc gccgaaggcg gcgaggactt
     4981 cgtcggcgtg ctgttcgacg ttgactttgt cgaagacctt tttgacgtgg ccctcttcgt
     5041 cgatcagaaa agtcttgcgg ctgacgccca tataggtttt gcccatgtag ttcttctcgc
     5101 cgtagacgcc gtaggcgtcg gagatcgtgt gatccgtgtc ggcgaggagc gtgaagggca
     5161 ggtcgtactt ggaggcgaac ttctggtgcg acgcctcgtc gtccagagag acgccgagga
     5221 ctttgatccc ctgctcgccg tacacgtcgt gcgcgtcgcg gaacgagcac gcctgcttcg
     5281 tgcagccggg cgtgtcgtcc ttcggataga agtagagcac gaccctctgc ccgcgcaagt
     5341 ccgagagctt cacctcgttg ccttccgcgt cgcgcgccgt gaagtcgggc gcggtgtcac
     5401 cttccttaat catctgttcg tcccccccgc ggtgttgttt tgtaagtcgt cgaagatgat
     5461 acagcgcgcc gctgtcgaat gcatacgcat aagaatcaac cacgaaggca caaagacacg
     5521 ggtaaagaag aaatcgcgtt tatctttacc cgtgtctttg tgccttcgtg gttgatttca
     5581 gtgttggccg ttccgctgga aggcgcggag gtgttcgggg atgaaggagg tgaactggtg
     5641 cgtgtcggcg cgcccgtcgg cgtaggtctg gtagagggtg aactcgggca ggcgcaggca
     5701 ggtgaggggc gaggcgtcgc tgtagcctgt gtcgatgccg atggcgctgt gcgcgacgta
     5761 gatgccgtgg cgcccgagcc gcccgaggag cggcaggagc ggcgtcggcg tgtggccgaa
     5821 gacgcagggc ttgccgtggt agtgcttgta gaagtacgag tcgcgcgtcc acaaaaggag
     5881 gcgcgcttcg gtctcgcagg ggtgcttctt gcagtcgagt ccggcgtgga cgtagatggc
     5941 gtagtcgtct tcgtggtaga gcggcaggct gcggaagaac tcgacgtgct cggcgggcac
     6001 ggcctctgtg atcttgcgcc gcgccttcgc gcagtcggcg cgcaggtgtt cggtgagggc
     6061 gaagcccgcg tactgctcga aggtgtcgtc gccgccgcac gcggggtgga gccagagcgg
     6121 cgcgcccgtg tccatgaagt cgaggagcat ctgctcgtgg ttgccgcgca gcacgacgac
     6181 gtggccggcg gcggggtcgc gcacgagcgc gagcgcgtcg cggacgacgc cgggcacgtc
     6241 gtcgccgcgg tcgatgaggt cgcccaggag aaccagcgtg tcgcgcgcgg ggtcgcgcgg
     6301 cagcatcccg acgaggcgcc gcaactgttc gcgccgcccg tgcacgtcgc cgatgacgaa
     6361 ggtgctcatg gccctcccgc tgttgcctcc ctttattgac tgcccgcgct gctcgtgccc
     6421 cccttcggtt cttccccccc ggattcgtct ttcttcgtgg ccgattttgc cggccccgcg
     6481 tgcgccgact cgatgcggcg catcaactcg cggaagcgcg ggtcgtcgcg cagtggttcc
     6541 cagttcgggt cggactcgaa ccacggctgg ttctcgttgc cgagcgtgat cgcgcgctcc
     6601 agccactcga aggcttcgtc ggcctcgccg agcatggcgt aggcggtggc cagccagtag
     6661 gccacgtcgt ggtcagccgc cgccgcgtcg agcacgcgct cggtcagctc gccgcgcgcc
     6721 gctgccacgt cgccggtctt gacgaggaac tgcgcgagga gcgggcgcac gccgtccatg
     6781 ccggggtggc gttcgaggac ttcgcgcatg agcttgatgg ccgccgcagg gtcgccgcgc
     6841 cgggagagga cgacggcgcg gaaggtcttg atgagcgggt ggttcggctc catctgcgcg
     6901 ccgaggtcga gttcggcgag cgcgtcgtcg tagcgcgact ggtacatcat cacgcgcgcg
     6961 cggttgtagc tcacgacgac tctttccgcc gggttcacct tgagcatccg gctgaagcct
     7021 tcgagcgcgt cgtcgtagcg cccgtcgagg cgcgcgagcg tggccgagac gaactgcacg
     7081 cccacgtcgt tcggcgcctc ggcgcgcatc agctcgaccc acgcgcgcgc cttctgcttc
     7141 tcgccgcgcg cgaggtagat gaagaccatg tggagccgcg cctccaacag cttcgggtcg
     7201 agcgcgagcg ccttctcgaa tgcctgctcg gcgcgctcgt agtcctcggg gccgccgaag
     7261 cccttgatga cgcggttggc gtaggcgccg ccgagcgcgc agtgcgcgag cgcgaagttg
     7321 gggtcgagtt cgagggcgcg ccggaagtgc cggatggcct cgtccgagtc ctcgcgcgag
     7381 agcgtgtgat agatgaagcg cccgcccgcg tcgcgcccgc gcaggtactc ttcgtaggcg
     7441 gcggcgctgg cggtggtgcg cgccgaggaa ggggcggcgt ccgccggggt gcggtcaacg
     7501 ctgagttcct gcgcgatggt ctggtcgatg gatttttcgg cctcggtctc gatgtggcgc
     7561 atcagctcgc ggaagcgcgg gtcgtcgtga agctcgcccc agttcgggtc ggactcgaac
     7621 cacgtgcggt tctcgttgcc gaggttgatg gcgcgctcca gccactcgaa ggcttcgtcg
     7681 gcctcgccga gcatggcgta ggcggtggcc agccagtagg cgatgtcgtg gtcggcgctg
     7741 gccgcgtcgc ggacgcggtc gttgagctgc gcgcgcgcct cgtcgcggtt gccctgccgg
     7801 acgagcatct gcgcgaggat ggggcggatg ccgtccatcg tcgggtgctc gtcgagcact
     7861 tggcgcaaga gtcgcgtcgc ctcgtccacg tcgcccttgc gcccgaggac gacggcgcgg
     7921 aaggtcttaa tcaaaggatg gtcgggctga atcttcgcgc cgtgttcgag ttcgagcagc
     7981 gcgtcgtcga agcggtgctg gtacatcatc acgcgcgcgc ggttgtagct ggtgacgacg
     8041 cgcgccgccg ggtcaaggcg cgacaggcgg tcgaaggcgc gcagcgcgcg gtcgtactcg
     8101 ccgtccagac ggtggagcgc gcccttgacg aagtagacgg cggcctcgtt cggggccttg
     8161 cgcgccagat gcgccacctc gtcgcgcgcc ttgcgcttct cgccgcgcga gaggtagatg
     8221 aagaccatca ggatgcgcgc ctcgacgagg ttcgggtcga ggtcgagcgc gcggctgaag
     8281 gcgttctcgg ccagctcgta gtc
//
LOCUS       DBNGOH010000919         2984 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_322908, whole genome shotgun sequence.
ACCESSION   DBNGOH010000919 DBNGOH010000000
VERSION     DBNGOH010000919.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2984)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2984)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2984
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_322908"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..364
                     /locus_tag="AC159P_17885"
     CDS             <1..364
                     /locus_tag="AC159P_17885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR3697368.1"
                     /translation="EAVVARFPANAALEGLRVPQVPRHALTFRAEYAAARRAHAFSVQ
                     GRFAGVQFDDDLNRFPLRRFFTLDALASRRLTDEVEIFAAAENLTGARYEVGRTPLTT
                     LGPPRFVRLGLRLRLGAR"
     gene            535..2940
                     /locus_tag="AC159P_17890"
     CDS             535..2940
                     /locus_tag="AC159P_17890"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HIR3697369.1"
                     /translation="MLKHVMTMTATVLALAAAVLNLSSTARAADAGFTTLDVNGEAVR
                     IYRDDFGTPHIFAETNRGLFEAYGYTVAQDRLWQLELNRRAARGRLAEVFGPGAGNSF
                     LNADRAARTTGYTGAELDAQFAMLTAEEQEIFDAYLGGINRYIGEAALNPLSRLPFEF
                     HALGFFPEPWTRRDSAAFGAFMVRRFGEIGGRELTNLSLLDSLIAAHGPANGFAIFND
                     VRWVNDPDSPVTVPSTGAFGKRQKKAGALNAAQLRDISAHRLEVSEEEAREAWASLGV
                     PTKLGSYAWAVSAARSDSGYAMLYGGPQMGFSVPEVLHEVQLKGGNGFNVAGMAFAGV
                     PPVLIGRNDHLAWTSTTATGDNLDHYIETTCNAGAGPGSGYVFNGACQPFEARVEVIN
                     VRGAAPVNHTVLRSVHGPVVGTSGANAITQKRAHWQRELESVHGFFGFDRARNIQQFE
                     AAVRLIPTSHNFIYADKGGNIAYWQAGQVPLRPAGFDARLPFPGDGSAEWPGGILPIP
                     TSINPAQGYLANWNNKPSVDYDNADNQIFGKQFRLWDIDDRLATGVISLEDMRDIPKD
                     IARVKGNGREARFLKPHLLAALNDVPPAHPLAAQAKAIVEAWDGNAFTDAVTSTSLQS
                     GEVIFSTWLSLMITNSFGDELGSRVNEASSNMLIHALDFALAGNSGVPPSRDYFNGVN
                     PKVVISATFDQTLAVLAAAQGNNPAAWTGPRGSITFTHPLVGVVASIPNSNRATYGQI
                     VVLGRPKITGESIFTLGQSGFLHLVLPNGFAFDPHFNDQLGLFRNFEYKPMRFFQNTQ
                     LKE"
BASE COUNT          519 a         1047 c          914 g          504 t
ORIGIN      
        1 cgaagcggtg gtggctcgct tccccgcgaa cgctgcgctc gaaggcttgc gcgtgccgca
       61 ggtgccgcgc cacgccttga ccttccgcgc cgagtacgcc gccgcacgac gcgcgcacgc
      121 cttctccgtg cagggacgat tcgcgggcgt gcagttcgac gacgacctca accgcttccc
      181 cctccgtcgg ttcttcacgc tcgacgcgct cgcctcgcgc aggctcacgg atgaagtgga
      241 aatcttcgcc gccgccgaga acctgacggg cgcgcgctac gaggtggggc gcacgccgct
      301 caccacgctc ggcccgcccc gcttcgtccg gctcggcctg cgcctccgcc tcggcgcgcg
      361 ctgagggcgt gtaagggact tgttaataat ccggccatct gctatttatt caccctcttt
      421 gcctgaacgg gtacgccaat tttttttcaa ctccactcga aaggagagcg ccccttctca
      481 tagagaagac gttctccctc gtgttcaacc tccagcagca taaaaggagc ggttatgctt
      541 aagcatgtca tgacgatgac cgcgacggtt ttggcccttg ccgccgccgt tctcaaccta
      601 tcctccacgg cgcgggccgc cgacgcgggt ttcacgacgt tggacgtgaa cggcgaagca
      661 gtcaggattt accgtgacga cttcggcacc ccgcacatct tcgccgagac caaccgaggg
      721 ctgttcgaag cttacggcta cacggtggcc caggatcgcc tctggcagct cgaactgaac
      781 cgccgcgccg cccgcgggcg gctcgccgaa gttttcggcc ccggcgccgg caacagcttt
      841 ttgaatgcgg atcgtgccgc gcgcacgacc ggctacacgg gcgccgaact cgacgcgcaa
      901 ttcgcgatgc tcaccgcgga ggagcaggag atcttcgacg cgtatcttgg cggcatcaac
      961 cgctacatcg gcgaggcggc gctcaacccg ctgtcgcggc tgcccttcga gtttcatgcc
     1021 ctgggtttct tcccggagcc ttggaccaga cgcgactccg ccgccttcgg ggccttcatg
     1081 gtgcgccgct tcggcgagat cggcgggcgc gagctgacca acctttcgct gctcgacagc
     1141 ctcatcgcgg cccacggccc cgcgaacggc ttcgccattt tcaacgacgt gcgctgggtc
     1201 aacgaccccg attcacccgt caccgtgccg agcacggggg cgttcggtaa acggcagaag
     1261 aaagccgggg ccttgaacgc cgcgcaactg cgcgacatct ccgcgcaccg gctggaggtg
     1321 tccgaggagg aggcgcgcga ggcctgggcg tcgctgggcg tgccgaccaa actcggcagc
     1381 tacgcctggg ccgtcagcgc ggcccgcagc gacagcggct acgccatgct ctacggcggg
     1441 ccgcagatgg gcttcagcgt gcccgaggtt ctccacgagg ttcagctcaa gggcggcaac
     1501 ggcttcaacg tcgcggggat ggccttcgcg ggcgtgcccc ccgtcttgat cgggcgcaac
     1561 gaccacctcg cgtggacttc cacgaccgcg accggagaca acctggatca ctacatcgaa
     1621 accacctgca acgcgggtgc cgggccgggt tcgggttacg tcttcaacgg cgcgtgccaa
     1681 ccgttcgagg cgcgggtcga ggtcatcaac gtgcgcggcg ccgcccccgt caaccacacc
     1741 gtcctgcgga gcgtgcacgg gccggtcgtc ggcaccagcg gcgcgaacgc catcacccag
     1801 aagcgcgcgc actggcagcg cgagttggag agcgtccacg gctttttcgg cttcgaccgc
     1861 gcgcgcaaca ttcagcagtt cgaggccgcc gtccggctga tccccacctc ccacaatttc
     1921 atctacgccg acaagggcgg gaacatcgcc tactggcagg cgggtcaggt gcccctgcgg
     1981 cccgcggggt tcgacgcgcg cctgcccttc cccggcgacg gcagcgcgga gtggcccggc
     2041 ggcatcctgc ccatcccgac ctcgatcaac ccggcgcaag ggtatctggc taattggaac
     2101 aacaaacctt cggtcgatta cgacaacgcg gacaaccaaa tcttcggcaa gcagttccgc
     2161 ctctgggaca tcgacgaccg gctggccaca ggagtcattt cgctcgaaga catgcgcgac
     2221 atccccaaag acatcgcgcg cgtgaaaggc aacgggcgcg aggcgcgctt cctgaagcct
     2281 cacctgctcg cggcgctcaa cgacgtgccg ccggcgcacc cgctcgccgc gcaggcgaag
     2341 gccatcgtcg aggcgtggga cggcaacgcc tttacggacg ccgtgaccag caccagcctc
     2401 caatccggcg aagtaatctt ttcgacgtgg ctcagcctga tgatcaccaa cagcttcggc
     2461 gacgagctgg gcagcagggt taatgaagcc agctcgaaca tgctcatcca cgctctggac
     2521 tttgccctcg cgggcaattc gggcgtcccg ccgagccggg attacttcaa cggagtgaat
     2581 ccgaaggtcg tcatctcggc cacgttcgac cagacgttgg cggtgctcgc cgcggcgcag
     2641 ggcaataacc ctgccgcgtg gacggggccg agggggtcga tcaccttcac ccacccgctc
     2701 gtcggcgttg tcgccagtat tccgaactcc aaccgcgcca cctacgggca gatcgtcgtg
     2761 ctcggacggc ccaaaataac gggcgagagc atcttcaccc tcggccagag cggcttcctg
     2821 catctggtct tgccgaacgg cttcgccttc gacccgcatt tcaacgacca actcgggctt
     2881 ttcaggaact tcgagtacaa gccgatgagg ttctttcaga acacccaact caaggagtag
     2941 gccgccgtcg gctgcttatt cttcgaggta gacaggactc ggca
//
LOCUS       DBNGOH010000920        13001 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3072727, whole genome shotgun sequence.
ACCESSION   DBNGOH010000920 DBNGOH010000000
VERSION     DBNGOH010000920.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 13001)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13001)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13001
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3072727"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..581)
                     /locus_tag="AC159P_17895"
     CDS             complement(<1..581)
                     /locus_tag="AC159P_17895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697370.1"
                     /translation="MSRMWGALACAVGLLVFAASAPPLTGAYAKARGVGSGVAQDRSV
                     VRRPIYQSAFEVEVLVNGYPLDALYGRGRRYVEAVAGEEYELRVRNPLPVRVAVALSV
                     DGLNTIDARHTASWDASKWVIAPYGTIRVGGWQMSSARARRFYFTTERDSYAARLGRK
                     SDLGVITAVFYREREYHTGPVPVTPRPRRDGEW"
     gene            complement(769..1269)
                     /locus_tag="AC159P_17900"
     CDS             complement(769..1269)
                     /locus_tag="AC159P_17900"
                     /inference="COORDINATES: protein motif:HMM:NF019499.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HIR3697371.1"
                     /translation="MARHGEALTVADEQAASGALKRAPLWDGAVALLALVGLVDAGYL
                     TTEHLAGRIGNVPCMVTTDCGGVLTSSYATLPGDIPLAALGALAYFVAFSLATLAAFG
                     YDSARKLLLPLVLTMFAASLWLLYVQAFVLEAFCSYCLLSAALSTTMMALVLVRRRLS
                     RKHDLS"
     gene            complement(1269..2012)
                     /locus_tag="AC159P_17905"
     CDS             complement(1269..2012)
                     /locus_tag="AC159P_17905"
                     /inference="COORDINATES: protein motif:HMM:NF024853.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HIR3697372.1"
                     /translation="MSRDKTSGAKRILPFVIIVGVLVAVVVGGVLALRKDEAKPPASS
                     YTAPPSSGTNATQPASSSSAPRRESPGAQPPHMKGRQSAPVVLEEFGDYQCPPCGMMH
                     PVLKKIGEDYGDRVAIVFRNFPLQKIHKNAFSASRAAEAAGMQGKFWQMNDLIYTNQK
                     AWSDSAEPRPLFESYARQLNLDIDKFKADSEKPETVSRILADFQRGDSLGVTGTPSIF
                     LNGRQLPVESALSEPRLRAEIDAALAAKK"
     gene            2235..2888
                     /locus_tag="AC159P_17910"
     CDS             2235..2888
                     /locus_tag="AC159P_17910"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3697373.1"
                     /translation="MNQIEATTQLPGGAISIYDRFTNLYDLMFRFNRYGRSIERYLRE
                     NPLPLPTDARVLDAGCGTGLLTLALLRVLKRPARVTAFDLSRRSLLTARRAARKAKAA
                     PRHRISFLQANALALPFADESFDFVVTSGVLEYLPLDEGMGELARVLKPGGYFFHLPV
                     RPSPATTMLEIMFRFKGHPPADITRHTERHFAVIEHHHFSPLEPIGWTKTAYLAQKP"
     gene            complement(2974..3960)
                     /locus_tag="AC159P_17915"
     CDS             complement(2974..3960)
                     /locus_tag="AC159P_17915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HIR3697374.1"
                     /translation="MARRISRAAPWLGLSVCLCFTVARAQQPDARRAQQRTAVSSSPR
                     LQRAVDEAARAALEKFGAKGFKETDLAVTVIDVTDPQRSEQASRRGAEPIYPASVVKI
                     FYLAAAHRWLEDGRLKETEELTRALRDMIVDSSNDATHYVVDALTGVSNGAELPPEEM
                     KQWSERRNAVNRYFASLGYPVGPGGINVNQKPWCEGPYGRERAFLGPKYENRNKLTTD
                     STARLVAEIATGRAVSPARSAKMLELMKRDFSGKSEDPDDQAHGFTAPALAPGTRYWS
                     KAGWTSTTRHDAAYLELPTGQRLVIVIYTTDHAKERDIIPTVARSILQAVVK"
     gene            4069..5421
                     /locus_tag="AC159P_17920"
     CDS             4069..5421
                     /locus_tag="AC159P_17920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015331096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HIR3697375.1"
                     /translation="MRREDARMEETTEAGLVRGIRKWDLVALIINSMVGAGIFVLPAR
                     AFGLIGPYSLLAFVVCALVVALIILCFAEVGSRFRETGGPYLYAREAFGPAAGFLVGW
                     MFWLARLTSSATNCNLLVVYLGHFWPAANDGGWRAAIITFVIVSLTAINYVGVRDATF
                     VSNLFAVGKLLPLLLFVAVGVFYIQPANFEAAALPATGSFSTAVMLLVYAFTGFENAS
                     VPTGEIRDPRRNMPVAILIAVGIVALFYISIQAVCVGTLPGLATSQRPIADAGGVFLG
                     AAGAALIVAGAVVSIIGNLNAGVLTTSRLPFAMAERGELPTALSATHRRFRTPHVSIL
                     LTGALLLALTLTSSVIYALTLSTIARLLAYAATCLALPVLRRREDTPPALFRVPGGVI
                     VSIAALLLCVWLLSQSTGREARDAAIAVAAGFVVYAVFRWKYRGEMKIKDAADHTAAP
                     "
     gene            complement(5625..7316)
                     /locus_tag="AC159P_17925"
     CDS             complement(5625..7316)
                     /locus_tag="AC159P_17925"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HIR3697376.1"
                     /translation="MKAVGKLSLFVFACVVMSNGLQRNASAQSFEADLVITNANVRTM
                     DAARPAAEAVAVHGNRIVAVGTSEEMKRHTGARTRVVDAKGALVLPGFNDAHVHFLSG
                     GFQLASVDLRDAATPREFAERIRRFAERLPPGRWITGGDWDHERWPSAPLPTKELIDS
                     FTPDRPVFVSRLDGHMALANSHALKLAGITRETPDPPGGLIVRDPSTGEPTGVLKDNA
                     MTAVYAKIPDPTFEEKLAAARAATEHAASLGVTSVQDVSAGSDVGVYQTLLERGELKT
                     RIYAVAPLGQWKRLADVGVHKAFGSDMLRIGGLKAFSDGSLGSTTALFFEPYADAPET
                     RGLGSGLAEGEMLKRVREADRAGLQVFIHAIGDRANDQILSIYEQVAREHGERDRRFR
                     IEHAQHLRAEEIREFARQKVVASMQPYHAIDDGRWAEKRIGPVRIKGTYAFRSLLDAG
                     AHLAFGSDWTVAPIDPLLGVYAAVTRRTLDGKNPGGWVPEQKITVEEAVRAYTHGSAY
                     AEFADAVKGTITPGKLADLVILSRDIFRIDPAEIEKARVTMTIVDGRVVYEKGSN"
     gene            7478..10372
                     /locus_tag="AC159P_17930"
     CDS             7478..10372
                     /locus_tag="AC159P_17930"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HIR3697377.1"
                     /translation="MDTLTQSLRAFLRRDQVITDPEELLVYECDGLTHYRARPRAVVL
                     PHSTEEVAEVLRVLAREGASFAPRGAGTGLSGGALAVNGGVQLNLARMRRVLKVDVEN
                     RLAVVETGMVNAQLSRAVARHGLHYVPDPSSQASCTIGGNIAENAGGIHCLKYGTTVD
                     HVLGARVVLSDGEVVELGGAGIESSGYDLLGVFVGSEGTFGVATEATVRLTPVAPSVR
                     TLLADFTDIDDASRAVSAIIAEGIIPAALEMVDGATIRAVEASVFAAGLPTDAEAALL
                     VELDGLDAGLDAEASRVEGICRASGARGVRHAADEVERKKLWAARKGAFGAMGRIMPD
                     MMLQDAVVPRSRLPEILADTYRIAAKYRLRLANVFHAGDGNLHPIMCYDARDRDEVER
                     VREAGREIVETCVRAGGTITGEHGVGLDKSDYLPLVFSEDTLRAMLQLRAAFDPSGRC
                     NPGKVIPVLRGCGEARAAVKQKSEVRSQKSESSSTSSATAVASAAHAAHAAATHVQTA
                     RLPSTSVARAAFHAERARAVLADIVGVANVETGAGETLFVAPSSAEEASELLLLSSRE
                     GWKVSPSGAARLPDACDAPSRADAQTSRVDETTSPVGIFISTKRMARVVEHEPADLVA
                     TAEAGVTLADFNATLAGAGQWLPLDPPGASSVTLGGVAARGLGGAQALGYGPPRSKVL
                     GMRVVLADGRVIRVGGRVVKNVAGYDLCKLFVESRGTLGLITELTFKLRPRPARESTL
                     VAHAADHRALLRAARAVVAAPLFPVAAELLSTRAAALGIPAIPDGCVLMLRFGGNAET
                     VSYQLSRAREMLDEHAAGAGVETFDDDDQHLWSGLAALTPEAARPLVFRASVLPGSLD
                     SLLAAARAYEPATGAAWHAGAGDGRLRVFADAPADGAASLSMLEELRRVSRELGGQLI
                     LERAPAEMRRAFDARAEASSSSTSLMRRVKQQLDPSGTFSAGSLFDDCGR"
     gene            10409..11839
                     /locus_tag="AC159P_17935"
     CDS             10409..11839
                     /locus_tag="AC159P_17935"
                     /EC_number="1.5.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014774.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HIR3697378.1"
                     /translation="MTDHATQLAGALKAEERKLLACVHCGLCLEACPTYAHTGDENDG
                     PRGRLYLMRAVEEERLPVTSPSFTRHINRCLGCRACESACPAGVEYGQLLEAARADIS
                     AHERSRGFTARLLGLALRHVWAHPSRLRLAFRLARALRDAGVPRLALGATMKRLLPAR
                     LVFAAALLDSSSPAPLVRDGEGATHSPAASTASTDDEASTSSAHVPATSSSVKASVSP
                     PDDVAGVGALLFEGCVTAGLFARVNRATARVLGANGCGTRAPEGQVCCGALHAHAGEL
                     EGARQLARRNVEAFGAGPPLPVVTNAGGCGAMLASYAQRLAHDAESAARARDFSARVR
                     DVSQQLAVTGLREGAGVGFGAATYDASCHLLHGQRAATEPLRVLGAVRGLRLVPLAGS
                     DVCCGGAGVYNLVEPELSARVLGEKLTHVRETDARILATGNPGCHMQLGAGARLRGLP
                     LRVCHPVELLDESYRRAGLYGPTDES"
     gene            12179..>13001
                     /locus_tag="AC159P_17940"
     CDS             12179..>13001
                     /locus_tag="AC159P_17940"
                     /inference="COORDINATES: protein motif:HMM:NF012234.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR3697379.1"
                     /translation="MSTKSISKFISEALCRPTDYISYSVSTKLAEVYPDRATVEGEST
                     FFDIEEFARAGHCELVGERAVHNQTSTAWQGADEPLLREADNGWFNVLWRGALLDVVF
                     ITWTESGYRSRFHWIVADTRELAEEFFREVCAWSAEVRGEILVFEGGSWRKSKELFRS
                     IREASFENLILPEPLRSEVERDLPRFFASREMYERHGVPWKRGVLLIGPPGNGKTHAV
                     KAMINSSQVPCLYVKSLDACDGEHASVRRVFWRARRSQPCVVVFEDVDSLINNKNR"
BASE COUNT         1943 a         4561 c         4343 g         2154 t
ORIGIN      
        1 tcccactcgc cgtcgcggcg cgggcgcggc gtgacgggca cggggcctgt gtggtattcg
       61 cgctcgcggt agaagacggc ggtgatgacg ccgaggtcgc ttttgcgccc gagccgcgcg
      121 gcgtaggagt cgcgctcggt ggtgaaatag aagcggcggg cgcgcgccga actcatctgc
      181 cacccgccga cgcggatcgt accgtacggc gcgatgaccc acttgctcgc gtcccagctc
      241 gcggtgtggc gcgcgtcaat cgtgttgagg ccgtccacag agagcgcaac ggcgacgcgc
      301 accggcagag ggttcctcac gcgcagctcg tactcctcgc ccgcgacggc ctcgacgtaa
      361 cggcgaccgc gaccgtagag cgcgtccaga gggtagccgt tgaccagcac ctcgacctcg
      421 aacgcgcttt gataaatggg ccgccgcacc acactcctgt cctgcgcgac gccggagccg
      481 acaccccgcg ccttcgcgta agcccccgtc agcggcggcg ccgacgccgc gaacaccagc
      541 agcccgaccg cgcaagccag agcgccccac atcctgctca tctcgaaaat cctccttctt
      601 cgggcagttt acacccgcac agcctttgaa cgcgcggcga aaagtattct tgcagggccg
      661 aaaggaaggg gcgaagaggg gaaatgggga aaaggaagaa ggaaccgctt tatgaagcgc
      721 tctgcttctc ccttttcgcc ctttcccctc ttcccctttt cccccttctt acgaaaggtc
      781 gtgtttccgg ctcagccgcc ggcggacgag gacgagcgcc atcatcgtcg tcgagagggc
      841 ggcggagagg aggcagtagg agcagaaagc ttcgaggacg aacgcctgca cgtagaggag
      901 ccagagcgag gcggcgaaca tggtgaggac gagcgggagc agtagcttgc gcgccgagtc
      961 gtagccgaag gcggcgagcg tcgccaggct gaaggccacg aagtaggcga gcgcaccgag
     1021 cgcggcgagc gggatgtcgc cgggcagcgt ggcgtagctg ctcgtcagca cgccgccgca
     1081 gtcggtcgtg accatgcagg ggacgttgcc gatgcgcccg gcgaggtgct cggtcgtcag
     1141 gtagcccgcg tcaaccagcc cgaccagcgc gagcagcgcg accgcgccat cccacagcgg
     1201 cgcacgtttc agcgcgccgc tcgccgcctg ttcgtccgcg accgtcaggg cttcgccgtg
     1261 ccgggccatt actttttagc cgcgagcgcg gcgtcgatct ccgcgcgcag acggggttcg
     1321 gagagcgccg actcgaccgg gagctggcga ccgttgagga agatcgaggg cgtgccggtg
     1381 acgccgagcg agtcgccgcg ctggaaatcc gcgaggatgc gcgagacggt ctcgggcttc
     1441 tccgagtcgg ccttgaactt gtcgatgtcg aggttgagct ggcgcgcgta gctctcgaag
     1501 agcgggcgcg gctcggcgga gtcgctccac gccttctggt tcgtgtagat caggtcgttc
     1561 atctgccaga acttgccctg catgccggcg gcctccgccg cgcgcgaggc cgaaaaggcg
     1621 ttcttgtgaa tcttctgcaa ggggaagttg cggaagacga tggcgacgcg gtcgccgtag
     1681 tcctcgccga tcttcttgag cacggggtgc atcatcccgc acggcgggca ctggtagtcg
     1741 ccgaactctt cgagcacgac cggcgcgctc tgccggccct tcatgtgcgg cggctgcgcg
     1801 cccggcgact cgcgccgcgg cgccgacgat gaagaggcgg gctgtgtcgc gttcgtgccc
     1861 gacgacggcg gcgccgtgta gctcgacgcc ggaggcttcg cctcgtcctt gcgcagcgcc
     1921 agcacccctc cgacgacgac ggcgaccagc acgccgacga tgatgacgaa cgggagaatc
     1981 cttttcgccc ccgaagtttt atcacgactc atcttgattt ttaccgtctc cgaaatttcg
     2041 gtctgtttcc agtaacgaac cgcgcccacg aacttttgcg cgacatgtta gcaccgccgc
     2101 gcgccgcaac ggaaacccgc gccgcttagc aaaagccgcc ggatgtgtta ctttttttcc
     2161 gcactcttgg gagccgaggc aattatcgcc cggctgtttt attatcttca ttcaacgcac
     2221 ggcgacggga actaatgaat caaatcgaag ccacaacgca actgcccggc ggggccatct
     2281 ccatctacga ccgcttcacc aacctctacg acctgatgtt ccggttcaac cgctacgggc
     2341 gctccatcga gcgctacctg cgggagaacc cgctgccgct gccgacggac gcgcgcgtcc
     2401 tcgacgcggg ctgcggcacc ggactgctga cgctcgcgct gctgcgcgtc ctcaagcgcc
     2461 cggcgcgcgt cacggccttc gacctctcgc gccgctcgct gctcaccgcg cgccgcgccg
     2521 cccgcaaggc gaaagcggcc ccgcgccacc gcatctcgtt cctacaggcc aacgccctcg
     2581 cgctcccctt cgccgacgag tcgttcgact tcgtcgtcac cagcggcgtg ctcgaatacc
     2641 tccccctcga cgaaggcatg ggcgagctgg ctcgcgtgct caagcccggc ggctacttct
     2701 tccacctccc cgtccggccc tcgcccgcga ccaccatgct cgaaatcatg ttccgcttca
     2761 aaggccaccc gcccgccgac atcacacgcc acaccgagcg ccacttcgcc gtcatcgagc
     2821 accaccactt ctcccccctc gaacccatcg gctggacgaa gacggcgtat ctcgcgcaga
     2881 agccgtaagg aagtcagaag tcagaacaaa aggcgtgctg attcggctcc gcactctcta
     2941 cgcttttcgt tctgacttct gacttctgac ttcctatttc accaccgctt gaaggatgga
     3001 acgcgcgacg gtcgggatga tgtcgcgctc tttggcgtgg tctgtggtgt agatgacgat
     3061 gacgaggcgc tgccccgtgg gcagttcgag gtaggcggcg tcgtggcgcg tggtggaagt
     3121 ccagccggcc ttcgaccagt agcgcgtgcc gggcgcgagc gccggcgcgg tgaagccgtg
     3181 cgcctgatcg tccgggtctt cggacttgcc ggagaagtcg cgcttcatca gttcgagcat
     3241 cttcgcgctg cgcgcggggc tgacggcgcg gcccgtggcg atctctgcga cgaggcgcgc
     3301 ggtcgagtcg gtcgtcagct tgttgcggtt ctcgtacttc gggccgagga aggcgcgctc
     3361 gcgcccgtac ggcccctcgc accacggctt ctggttgacg ttgatgccgc ccggcccgac
     3421 cgggtagccg agcgaggcga agtagcggtt gacggcgttg cgccgctccg accactgctt
     3481 catctcttcg ggcggaagct ccgcgccgtt cgagacgccc gtcagcgcgt ccaccacgta
     3541 atgcgtcgcg tcgttcgaag agtccacgat catgtcgcgc agcgcgcgcg tcagctcctc
     3601 cgtctccttc agccgcccgt cctccagcca gcggtgcgcc gccgcgaggt agaaaatctt
     3661 caccacgctc gccggataga tcggctccgc gccgcgccgg ctcgcctgct ccgaccgctg
     3721 cgggtcggtc acgtcgatga ccgtgacggc gaggtccgtc tccttgaaac ccttcgcgcc
     3781 gaatttttcc aacgcggcgc gcgcggcctc gtccacggcg cgttgcagtc tgggcgatga
     3841 agagacggcg gtgcgttgtt gcgcccggcg cgcgtcaggc tgctgcgccc gcgcgaccgt
     3901 gaaacacaga caaaccgaca gcccgagcca cggggccgcc ctcgaaatcc gacgtgccat
     3961 caatttccgc accctttccg aatgactggt atactcgcct cgcggggcgg cgcgcttcta
     4021 tctaaagtac gccgccccgt cacgccacag aagagaccac gaacaatctt gagacgcgag
     4081 gacgcgcgca tggaagagac gaccgaagcc gggctggtcc gaggcatccg caagtgggac
     4141 ctcgtcgcgc tcatcatcaa cagcatggtc ggcgcgggca tcttcgtgct gcccgcgcgg
     4201 gccttcggtc tgatcggccc ttacagcctg ctcgccttcg tcgtctgcgc gctggtcgtc
     4261 gcgctcatca tcctctgctt cgcggaggtc ggcagccgct tccgcgagac gggcggcccg
     4321 tatctttacg cgcgcgaggc gttcggcccc gccgccgggt ttctggtcgg ctggatgttc
     4381 tggctcgcgc ggctcacctc gtcggccacc aactgcaacc tgctcgtcgt ctacctcggc
     4441 cacttctggc cggcggcgaa cgacggcggc tggcgggcgg ccatcatcac cttcgtcatc
     4501 gtctcgctga cggcgatcaa ctacgtcggc gtgcgcgacg cgaccttcgt cagcaacctg
     4561 ttcgccgtcg ggaagctgct gccgctgctg ctcttcgtcg ccgtcggcgt cttctacatc
     4621 cagcccgcca acttcgaggc ggcggcgctg cccgcgaccg gctctttctc gaccgccgtg
     4681 atgctgctcg tctacgcctt cacgggtttc gagaacgcga gcgtgcccac cggcgagata
     4741 cgcgacccgc ggcgcaacat gcccgtcgcc atcctcatcg ccgtcggcat cgtcgcgctt
     4801 ttctacatct cgattcaggc cgtttgcgtc ggtacgctgc ccggtctggc gacctcgcag
     4861 aggccgattg ccgacgcggg cggcgtgttc ctcggcgcgg cgggcgcggc gctcatcgtg
     4921 gcgggcgccg tcgtctccat catcggcaac ctcaacgcgg gcgtgctgac cacgtcgcgg
     4981 ctcccgttcg ccatggcaga gcgcggcgag ctgcccaccg ccctctcggc cacgcaccgc
     5041 cgcttccgca cgccgcacgt ctcgatcctg ttgacgggcg cgctgctcct cgcgttgacg
     5101 ctcaccagct ccgtcatcta cgcgctgacg ctctcgacca tcgcgcgcct gctcgcctac
     5161 gccgcgacgt gcctcgcgct tcccgtcctg cgtcggcgcg aagacacgcc cccggcgttg
     5221 ttccgcgtgc ccggaggtgt cattgtttcc atcgctgcgc tgctgctctg cgtctggctg
     5281 ctctcgcaaa gcaccgggcg cgaggcgcgc gacgcggcca tcgccgtcgc cgcgggcttc
     5341 gtcgtctatg ccgtcttcag atggaagtac cgaggggaga tgaaaatcaa agacgccgcc
     5401 gaccacacag ccgctcctta gaagccgtca atgaaccttc cgcccctcgc cccgagagca
     5461 ggtttcaggt gatcgtacaa cgcgcatgaa gactgtaggg gcagggcaag tccgcgtccc
     5521 gcggtcgcat tacgtcaaac ctcgaatcca tcgcgagcag cgggtaggga caagccctgc
     5581 ccctacagtc ttcacgcgcg taccgcaacc acctgaaact tgctctagtt gcttcctttt
     5641 tcgtagacca cgcgaccgtc aacaatcgtc atcgtcacgc gcgccttctc tatctcggcg
     5701 gggtcgatcc tgaaaatgtc gcgggagagg atgacgaggt ctgcgagctt gccgggcgtg
     5761 atggtgcctt tgacggcgtc ggcgaactcg gcgtaggccg agccgtgcgt gtaggcgcgc
     5821 accgcctctt cgactgtgat cttctgttcg ggcacccacc ctcctgggtt cttgccgtcg
     5881 agcgtgcggc gcgtgactgc ggcgtagacg ccgagcagcg ggtctatcgg cgccaccgtc
     5941 cagtcggagc cgaaggcgag gtgtgcgccc gcgtcgagca gcgagcggaa ggcgtaggtg
     6001 cccttgatgc ggacggggcc gatgcgcttc tcggcccagc gaccgtcgtc gatggcgtgg
     6061 tagggctgca tcgaggcgac gaccttctgg cgcgcgaact cgcgtatctc ttccgcgcgg
     6121 aggtgctgcg cgtgctcgat gcggaagcgg cggtcgcgct cgccgtgctc gcgcgcgacc
     6181 tgctcataaa tcgaaagtat ctggtcgttg gcgcggtcgc cgatggcgtg gatgaagacc
     6241 tgtaggcccg cgcggtcggc ctcgcggacg cgcttcagca tctcgccttc ggcgagaccg
     6301 ctgccgagtc cacgcgtctc gggcgcgtcc gcgtatggct cgaagaagag cgccgtggtc
     6361 gagccgaggc tgccgtcgga gaaggctttg aggccgccga tgcggagcat gtcggagccg
     6421 aacgccttgt gcacgcccac gtccgcgagc cgcttccact ggccgagcgg cgccaccgcg
     6481 tagatgcgcg tcttcagctc accgcgctca agcagcgttt gatagacgcc gacatcgctg
     6541 cccgccgaca cgtcctgcac gctcgtcacg ccgaggctcg ccgcgtgctc ggtcgccgcg
     6601 cgcgccgccg ccagcttctc ctcgaacgtc gggtcgggga ttttcgcgta gaccgccgtc
     6661 atcgcgttgt ctttgagcac gccggtcggc tcgcccgtcg aggggtcgcg cacgatgagt
     6721 ccgcccggcg ggtcgggcgt ctcgcgcgtt atccctgcga gcttcaaggc gtggctgttg
     6781 gcgagcgcca tgtgcccgtc gaggcggctg acgaagacgg gccgatccgg cgtgaacgag
     6841 tcgatcaact ccttcgtcgg cagcggcgcc gacggccaac gctcgtgatc ccagtcgccg
     6901 cccgtaatcc aacgccccgg cggaagcctc tccgcgaaac gccggatgcg ctcggcgaac
     6961 tcgcgcggcg tggcggcgtc gcgcaagtcc acgctcgcga gctgaaatcc gcccgacaga
     7021 aaatgcacgt gcgcgtcgtt gaagcccggc agcacgagcg cgcccttcgc atcgaccacg
     7081 cgagtgcgcg cgcccgtgtg ccgtttcatc tcttccgacg tgccgaccgc gacgatgcgg
     7141 ttgccgtgaa ccgcgacggc ctcggcggcg ggacgcgcgg cgtccatcgt ccgcacgttc
     7201 gcgttcgtga tgacgaggtc ggcctcgaac gactgcgccg acgcgtttct ttgcagaccg
     7261 ttgctcatga cgacacacgc gaagacgaac agggagagtt ttccgactgc tttcatgtgg
     7321 gcgattttct gctggcgaac ggggcttccg aacaggctgc gccgcctgac tcacggggcc
     7381 gataccgggc gcgtgttact caaacggcgt ggggctattg tatcattgag cctccgccgt
     7441 tcggcagtta aaagttctcc gccgccgcca tacctttgtg gatactttaa cccagagcct
     7501 gcgcgccttc ctgcgccgag atcaggtcat cacggacccc gaagagctgc tcgtctacga
     7561 gtgcgacggg ctgacgcatt accgcgcgag gccgcgcgcc gtcgtgctcc cgcattcgac
     7621 cgaagaggtg gccgaggtgt tgcgcgtgct cgcgcgcgag ggcgcgtcgt tcgcgccgcg
     7681 cggggcgggc acgggactgt cgggcggggc gctcgccgtg aacggcggcg tgcagctcaa
     7741 cctcgcgcgg atgcggaggg tgttgaaggt cgatgtggag aatcgactcg cggtggtcga
     7801 gacggggatg gtcaacgcgc agctctcgcg cgcggtcgcg cggcacgggc tgcattacgt
     7861 gcccgaccct tcgagtcagg cgtcgtgtac catcggcggc aacatcgccg agaacgcggg
     7921 cggcatccat tgtctgaagt acggcacgac ggtcgatcac gtgctcggcg cgcgcgtcgt
     7981 gctgtcggac ggcgaggtgg tcgaactcgg cggcgcgggg attgaatcaa gcggctacga
     8041 cctgctcggc gtgttcgtcg gctccgaggg cacgttcggc gtggcgaccg aggcgaccgt
     8101 caggctcacg ccggtcgcgc cgtctgtgcg gacgctgctc gccgacttca ccgacatcga
     8161 cgacgcaagc cgcgccgtct cggccatcat cgcggaaggc atcatccccg ccgcgctgga
     8221 gatggttgac ggcgcgacca tccgcgcggt cgaggcgagc gtcttcgccg cgggcctgcc
     8281 gacggacgcg gaggccgcgc tgctcgtcga gcttgacggg ctggacgcgg gactcgacgc
     8341 ggaggcttcg cgcgtcgagg gcatctgccg cgcgtcaggc gcgcgcggcg tgcgccacgc
     8401 cgccgacgag gtcgagcgca agaagctgtg ggcggcgcgc aagggcgcgt tcggcgcgat
     8461 gggccgcatc atgcccgaca tgatgttgca ggacgcggtc gtcccgcgct cgcgtctgcc
     8521 cgagattctg gccgacacct accgcatcgc cgcgaagtac cggttgcgtc tcgccaacgt
     8581 cttccacgcg ggcgacggca acctccaccc gatcatgtgc tacgacgcgc gcgaccgcga
     8641 cgaggtcgaa cgcgtgcgcg aagcgggccg cgagatcgtc gagacgtgcg tgcgcgcggg
     8701 cggcaccatc acgggcgagc acggcgtcgg cctcgacaag agcgactacc tcccgctcgt
     8761 cttctccgaa gacactttgc gcgcgatgct gcaactgcgc gcggccttcg acccctccgg
     8821 gcgctgcaac cccggcaagg tcatccccgt cctgcgcggc tgcggcgaag cgcgcgccgc
     8881 tgtcaaacag aagtcagaag tcagaagtca gaagtcggaa tcgtcctcga cttcgtccgc
     8941 aacagccgtt gcctccgccg cgcacgccgc ccatgccgcc gcgacgcatg ttcaaacagc
     9001 acgcctccca tcaacctccg tcgcgcgcgc cgcctttcac gcggaacgtg cgcgagcggt
     9061 gctggccgat atcgtcggcg ttgcgaacgt cgagacggga gcgggcgaga cactcttcgt
     9121 cgctccctct tcggcggaag aggcgagcga actcctgctc ctgtcgtcgc gcgaagggtg
     9181 gaaggtctcg ccgtcgggcg ccgcgcgttt gcccgacgcg tgcgacgcgc cttcgcgtgc
     9241 cgacgcacag acttcgcgtg ttgacgaaac gacttcgcca gtcggtatct tcatcagcac
     9301 caagcgcatg gcccgcgtcg tcgagcacga gcctgccgac ctggtggcga ccgcggaggc
     9361 gggcgtgacg ctcgcggact tcaacgcgac gctcgcgggc gcgggccagt ggctcccgct
     9421 cgacccgccg ggcgcaagct ccgtgacgct cggcggcgtg gccgcgcgcg gactcggcgg
     9481 cgcgcaggcg ctcggctacg gcccgccgcg ctcgaaggtg ctcggcatgc gcgtcgtgct
     9541 cgcggacggg cgcgtcatcc gcgtcggcgg gcgcgtcgtg aagaacgtcg ccggctacga
     9601 cctctgcaag ctcttcgtcg agagccgcgg cacgctcggc ctcatcacgg agctgacctt
     9661 caagctgcgc ccgcgccccg cccgcgagag cacgctggtc gcgcacgccg ccgatcaccg
     9721 tgcgttgctc cgcgcagcgc gcgccgtcgt cgccgcgccg ctcttccccg tcgccgcaga
     9781 gcttctttcg acgcgcgccg ccgccctcgg catccccgcc atacccgacg gatgcgtgct
     9841 catgctccgc ttcgggggca acgccgaaac ggtctcgtac caactgagcc gcgcgcgcga
     9901 gatgctggac gagcacgcgg cgggcgccgg agtcgaaacc ttcgacgacg acgatcaaca
     9961 tctctggtcg gggctggccg cgctgacgcc ggaggcggcg cggcccctcg tctttcgcgc
    10021 gagcgtcctg cccggcagtc tcgattctct gttggcggcg gcccgcgcct acgaacctgc
    10081 gacgggcgcg gcgtggcatg cgggcgcggg cgacggtcgg ctccgcgttt tcgccgacgc
    10141 gcctgcggac ggcgcggcga gtttatcgat gctcgaagaa ctccggcgcg tgtcgcgcga
    10201 gcttggcggt caactcatcc tggagagagc gcccgccgag atgcgccgcg cgttcgacgc
    10261 gcgggccgaa gcgtcttctt cttccacgtc gctcatgcgg agagtgaaac aacagctcga
    10321 cccgtcgggc acgttctccg caggaagttt gttcgatgac tgcggacggt gaatgattcg
    10381 aaaccggcgt cgagagttga cgaacgagat gactgaccac gcgacacaac tggcgggcgc
    10441 gctgaaggcg gaggagcgga agctgctcgc ctgcgtgcac tgcggcctgt gcctcgaagc
    10501 ctgcccgacg tacgcgcaca cgggcgacga gaacgacggg ccgcgcggtc ggctctacct
    10561 gatgcgcgcc gtcgaagagg agcgcttgcc cgtcacttcc ccttcgttca cgcggcacat
    10621 caaccgctgc ctcggctgcc gcgcgtgcga gagcgcgtgc cccgcgggcg tcgagtacgg
    10681 acagttgctc gaagcggcgc gcgcggacat ctccgcgcac gagcgcagcc gcggcttcac
    10741 cgcgcggctg ctcggcctcg cgctccgcca cgtctgggcg cacccgtcgc ggctgcgcct
    10801 cgccttccgc ctcgcgcgcg cgctgcgcga cgcgggcgtc ccgagactcg cgctcggggc
    10861 gacgatgaag aggctgctgc ccgcgcgtct cgtgttcgcc gcggcgctgc tcgacagctc
    10921 ttcgcccgcc ccgctcgtcc gagacggcga aggcgcgacg cactcgcccg cagcgtcaac
    10981 ggcttcgacg gatgacgaag cctcaacgtc ttcagcgcac gtccctgcaa cttcgtcgtc
    11041 cgtcaaagca agcgtctcgc ctccggatga cgtggccggc gtcggcgcgc tgctcttcga
    11101 gggctgcgtg accgcggggc tgttcgcgcg cgtgaaccgc gcgacggcgc gcgtgctcgg
    11161 ggcgaacggg tgcgggacgc gggcgccgga ggggcaggtc tgctgcggcg cgctgcacgc
    11221 gcacgcgggc gagcttgagg gcgcgcggca gttggcgcga cgcaacgtcg aagccttcgg
    11281 cgcggggccg ccgctgcccg tcgtcacgaa cgcgggcggc tgcggggcga tgctcgcgtc
    11341 ctacgcgcaa cggctcgcgc acgacgcgga gtccgccgcg cgcgcgcgcg acttcagcgc
    11401 gcgcgtccgc gacgtgagcc agcagctcgc cgtgacgggc ctgcgcgaag gcgcgggcgt
    11461 cggcttcggc gcggccacct acgacgcctc gtgccacctg ctgcacggcc agcgcgcggc
    11521 gaccgagccg ctgcgggtgc tcggcgccgt gcgcggcctg cgcctcgtgc cgctcgcggg
    11581 cagcgacgtg tgctgcggcg gcgcgggcgt ctacaacctc gtcgagccgg aactttcggc
    11641 gcgcgtgctc ggcgaaaagc tcacgcacgt ccgagagacc gacgcgcgca tcctcgccac
    11701 cggcaacccc ggctgccaca tgcagctcgg cgcgggcgca cgcctgcgcg gtctaccctt
    11761 gcgcgtctgc caccccgtcg aactcctcga cgagtcctac aggcgcgccg gcctctacgg
    11821 cccgaccgac gagtcctgaa cccccgccac actcacgaac ccgccgcgcg atggctttga
    11881 cggaacagcg cccgcggatg attttttcgt tgacgcgcga cgaaaacggc ggtatggtgt
    11941 gtagaacttt gtaaagtaaa gtggttggcc gctatgcttg ttaccctgca tcccactcgc
    12001 tgatctccga ccgcgacccg cttgcactgg tcgcgccctc gaagtgtatc cgggcattcc
    12061 accgatattt tagcagccgc gcgcgctcga accttcgggt tcaagccctt tccagccgcg
    12121 cgcggacggc gtaactcgtg cggcgggccg cccgctcgaa gccggccttt gcgaaaagat
    12181 gtcaacgaag tcgatcagca aattcatcag cgaggcgctg tgccgcccga cggactacat
    12241 ctcttactcg gtgagcacga agctggcgga ggtgtatccg gacagagcaa ccgtcgaagg
    12301 ggagagcacc ttcttcgaca tcgaagagtt cgcgcgcgcg ggccactgcg agttggtcgg
    12361 cgagcgcgcc gtccacaacc agacctcgac cgcgtggcag ggcgcggacg agccgctcct
    12421 gcgcgaggcg gacaacggct ggttcaacgt cctctggcgg ggcgcgctgc tcgacgtggt
    12481 cttcatcacg tggacggaga gcggctaccg ctcgcgcttc cactggatcg tcgccgacac
    12541 gcgcgagctg gccgaggagt tcttccgcga ggtctgcgcg tggagcgccg aggtgcgcgg
    12601 cgagattctg gtcttcgagg gcggctcctg gcggaagagc aaggagctgt tccgctcgat
    12661 cagggaggcg agcttcgaga acctgatcct gcccgagccg ctgcgctccg aggtcgagcg
    12721 cgacctgccg cgcttcttcg cctcgcgcga gatgtacgag cggcacggcg tgccttggaa
    12781 gcgcggcgtg ctcctgatcg gcccgcccgg caacggcaag acgcacgccg tcaaggcgat
    12841 gatcaacagc tcgcaggtgc cgtgcctcta cgtgaagagc ctcgacgcct gcgacggcga
    12901 gcacgcgagc gtccgccgcg tcttctggcg cgcgcgccgc tcgcagccgt gcgtcgtcgt
    12961 cttcgaggac gtggactcgc tgatcaacaa caagaaccgc t
//
LOCUS       DBNGOH010000921         5783 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3439006, whole genome shotgun sequence.
ACCESSION   DBNGOH010000921 DBNGOH010000000
VERSION     DBNGOH010000921.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5783)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5783)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5783
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3439006"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..348
                     /locus_tag="AC159P_17945"
     CDS             <1..348
                     /locus_tag="AC159P_17945"
                     /inference="COORDINATES: protein motif:HMM:NF012618.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HIR3697380.1"
                     /translation="DVPAPHKRERLEKRKRLELLEGHKGDVQTLAFSPDGRTLATGSV
                     DQTVRLWNTFTHRLLATLEGHVTPVLSVAFAPGGGQLATADREGKVKVWRAATDEDVK
                     SQCIKCRRPAGSP"
     gene            419..1768
                     /locus_tag="AC159P_17950"
     CDS             419..1768
                     /locus_tag="AC159P_17950"
                     /inference="COORDINATES: protein motif:HMM:NF013998.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HIR3697381.1"
                     /translation="MSTLSETRIVRFVFTKTFYALLLVGFVPLSLSWGNPTLKWLAVL
                     YDVGLLLLAIVDANVSRLPQGIEVVREFGGRFHIGAETEVRINVVNNTARDFVLKVKD
                     EYPAEMALTGQREARMQVPARTTAALLYALEPPRRGLFEFGATAVRFVSRFRLVWCEA
                     NLGAATTTVKVYPNLRRAREAELKALGARSLVAAHRRAVWRGEGRDFESLREYVPGDE
                     LRHVSWTATARRGKLTTRQYQIERDQTVLVALDAGRLMTARIENETKFDSAIHAALAL
                     TSAAERGGDMAGLAVFGRRIRAFVPPAKGRERTDAVLEALHAIEPELIEPSYTRAFEF
                     VAANCKRRALVCVLTDLVDEEGSRELLQSLRILRPRHLPLVVTISDRDLRAAVRDTPD
                     TTDQLFVQSVAEEIMHQREAALRLVESQGGLALDVTTAALVPALLETYIRVKERGML"
     gene            complement(1849..2832)
                     /locus_tag="AC159P_17955"
     CDS             complement(1849..2832)
                     /locus_tag="AC159P_17955"
                     /inference="COORDINATES: protein motif:HMM:NF014052.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030435 - sporulation resulting in
                     formation of a cellular spore [Evidence IEA];
                     GO_process: GO:0042173 - regulation of sporulation
                     resulting in formation of a cellular spore [Evidence IEA];
                     GO_process: GO:1901893 - positive regulation of cell
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HIR3697382.1"
                     /translation="MSSDRFIDERKNAWQRLEDLLRMLDRSSLRRLHREEVRELGRIY
                     RRTASDLAIARAESRDPRLVNYLNSLVIRAHGRIYRADAAEGGRRIRKFFAREFPRTF
                     RRTWRFTALAFGVFMLFFAFAFVATQLDAGFSEFGGVSPVGREDILRNRPRWWEDLND
                     ANQIGASFIMVNNILVMFRAFAYGALFGFGTLYEMARFGVHVGSILSLTYLAGYGNDL
                     LTFMSAHGVIELSCIFIAGGAGMLFGSAVLMPGDLSRSDALRLRGRDAIRLVIGCVPL
                     LVVAGVIEGFISPALISPAVKFAVAGATFVALYTYLVFAGRETEAEEGGKG"
     gene            2929..3615
                     /locus_tag="AC159P_17960"
     CDS             2929..3615
                     /locus_tag="AC159P_17960"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3697383.1"
                     /translation="MKRKSVAALYDRIGGKYDENKSRAISDYTELPAVLSLAGDVRGL
                     SVLDAGCGPGRHAEKLLDRGALVTGIDVSEEMVGLARERCKGRGEFLRADFETAPFAP
                     STFDLVTASLCLMYSKDVRPAIRNFSRWLKHGGRLVFSLYHPVRFFVKIPDFDFSKSR
                     KVWIHLEGCDVTVFNYYHPLPRYFDALAESGLEVLRLVEPTLSRRHKGWPEDFYRIPR
                     SLVVAARKRS"
     gene            3673..4167
                     /locus_tag="AC159P_17965"
     CDS             3673..4167
                     /locus_tag="AC159P_17965"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF025793.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HIR3697384.1"
                     /translation="MGDELNDRDIELVRRAVARALEAERGGNLPIGSVLAVGGEVVAE
                     AGNAVLVPVYHPGRHAEKEALARVPASLWPRAREMTCYTTLEPCVMCMGALLLHGVGR
                     VVYGARDPEGGGGALLAHLPRYYAGGAGVPLWLGPALADVCDPLYERSRARFDALACG
                     RDNF"
     gene            4227..5087
                     /gene="bla"
                     /locus_tag="AC159P_17970"
     CDS             4227..5087
                     /gene="bla"
                     /locus_tag="AC159P_17970"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="subclass B3 metallo-beta-lactamase"
                     /protein_id="HIR3697385.1"
                     /translation="MKLTAALVLLLLFSSQAFAQMDETDRRRNAPVEPFRVIDNVYYV
                     GASDVTAFLVTTPRGHILIDAGYAETAPQIKANVARLGFKIEDVKILLTTQAHFDHAG
                     GLAELKRASGARLLASREDAALMANGGKGDFAFGDRFPYEAVKTDKTIADGEKIGLGG
                     VTLKAVSTPGHTKGCTAYTTTVREGGKKYEVVFFGSTTAPGYKLVGNEQYPNLVADYE
                     RTFRVSKALKPDVFLASHGSFFDLAGKAERARQGAAPNPFIDPESYRRYIGETEKTFR
                     EQLLKETRAK"
     gene            5310..>5783
                     /locus_tag="AC159P_17975"
     CDS             5310..>5783
                     /locus_tag="AC159P_17975"
                     /inference="COORDINATES: protein motif:HMM:NF025006.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HIR3697386.1"
                     /translation="MRSPSIRNLVECFRRGGLALVFIFALAPAAALAQSQITTGTVQG
                     TIEDETGASVPGANVEARNVETGLARSQTTGDDGRFVFLQLAPGRYTVTVSKSGFATI
                     EQEAFPLTVGQTVSLDLRMKVSGVAEKVTVSASDIATVDTTKTEASTTINRRSVEN"
BASE COUNT          981 a         1961 c         1965 g          876 t
ORIGIN      
        1 gacgtgcccg cgccgcataa gcgcgaacgg cttgaaaagc gcaaacggct tgaactgctc
       61 gaagggcaca agggggatgt ccagactttg gccttctcgc ccgacgggag gacgctcgcc
      121 acgggcagcg tcgatcagac cgtgcggctg tggaacacct tcacgcaccg cctgctggcg
      181 acgctcgaag gacacgtgac gcccgtgctc tccgtggcct tcgcgcccgg cggcggccag
      241 ctggcgaccg ccgaccgtga agggaaggtg aaagtctggc gcgcggccac cgacgaggac
      301 gtgaagtcgc agtgcatcaa atgccgccgg cccgccgggt ctccgtgacg gcccgcctgc
      361 cgaacgcggg aagcgcgcgc ctgcattgac ggcgcgcggc gggcggggca aactggcctt
      421 gtccacgctc agtgaaactc gcatcgtgcg cttcgtcttc accaaaactt tctacgcgct
      481 gctgctcgtc ggcttcgtgc cgctctctct gtcgtggggc aacccgacgc tgaagtggct
      541 ggccgtgctc tacgacgtgg ggctgctcct gctggcgatt gtcgatgcca acgtgagccg
      601 cctgccgcag ggcatcgagg tggtgcgcga gttcggcggt cgcttccaca tcggcgctga
      661 gaccgaggtg cgcatcaacg tcgtcaacaa cacggcgcgc gacttcgtgc tcaaggtcaa
      721 ggacgagtac ccggcggaga tggccctgac cggacagcgc gaggcgcgca tgcaggttcc
      781 ggcgcggacg accgccgcgc tcctctacgc gctcgagccg ccgcgccgcg gcctcttcga
      841 gttcggcgcg acggcggtgc gcttcgtctc gcgcttccgc ctcgtgtggt gcgaggcgaa
      901 tctgggggcg gcgacgacga cggtcaaggt ctacccgaac ctgcggcgcg cgcgcgaggc
      961 ggagctgaag gcgctcggcg cgcgctcgct ggtcgcggcg caccggcgcg cggtgtggcg
     1021 cggcgagggg cgcgacttcg aatctttgcg cgagtacgtg ccgggcgacg agctgcgcca
     1081 cgtctcgtgg acggcgacgg cgcggcgcgg caagctgacc acgcggcagt accagatcga
     1141 gcgcgaccag accgtgctcg tcgctttgga cgcgggccgc ctgatgacgg cgcgcatcga
     1201 gaacgagacc aagttcgact cggccatcca cgcggcgctc gcgctgacga gcgcggcgga
     1261 gcgcggcggc gacatggcgg gcctcgccgt cttcggtcgc agaatccgcg ccttcgtccc
     1321 gcccgccaag gggcgcgagc gcacggacgc cgtcctcgaa gccttgcacg ccatcgaacc
     1381 cgagctgatc gagccttcct acacacgcgc cttcgagttc gtcgccgcca actgcaagcg
     1441 ccgcgcgctc gtctgcgtcc tcacagacct cgtggacgag gaaggctcgc gcgagctgct
     1501 ccaatccttg cgcatcctcc ggccccgcca cctgccgctc gtcgtcacca tctccgaccg
     1561 cgacctgcgc gccgccgtgc gcgacacgcc cgacaccacc gaccagctct tcgtccaatc
     1621 cgtcgccgaa gagatcatgc accagcgcga agccgccctc cggctcgtcg agtcgcaagg
     1681 cggcctcgcc ctcgacgtga ccaccgccgc cctcgtcccc gcgctcctcg aaacctacat
     1741 ccgggtcaaa gagcgcggaa tgctttgaag ggggaaaagg ttaaaagggg aaaaggggaa
     1801 aaggaagaaa cggcaagtcg cttgtcttcc ctttcccctt ttcccctttt aaccttttcc
     1861 cccttcttcc gcctccgtct cgcgtcccgc gaagacgaga tacgtgtaga gtgcgacgaa
     1921 ggtggcgccc gcgacggcga acttgacggc gggcgagatg agggcgggcg agatgaagcc
     1981 ttcgatgacg ccggcgacga cgagcagcgg gacgcagccg atgacgaggc ggatggcgtc
     2041 gcgccctctg aggcgcagcg cgtccgagcg cgacaggtcg cccggcatca gcaccgcgct
     2101 gccgaagagc atcccggcgc cgcccgcgat gaagatgcag gacagctcga tgacgccgtg
     2161 cgcggacatg aaggtcagca ggtcgttgcc gtagccggcg aggtaggtga gcgagaggat
     2221 cgagccgacg tggacgccga agcgcgccat ctcgtagagc gtgccgaagc cgaacagggc
     2281 gccgtaggcg aaggcgcgga acatcaccag gatgttgttc accatgatga agctggcgcc
     2341 gatctggttc gcgtcgttca ggtcttccca ccagcgcggg cggttgcgga ggatgtcctc
     2401 gcgcccgacc ggcgacacgc ccccgaactc ggagaagccc gcgtcgagct gcgtggcgac
     2461 gaaggcgaag gcgaagaaga gcatgaagac gccgaaggcg agcgccgtga agcgccacgt
     2521 gcggcggaac gtgcggggga actcgcgcgc gaagaacttg cggatgcggc ggcccccttc
     2581 ggcggcgtcg gcgcggtaaa tgcgaccgtg cgcgcggatg acgaggctgt tgaggtagtt
     2641 gacgagccgc gggtcgcgcg actcggcgcg cgcaatcgcc aggtccgacg ccgtgcgccg
     2701 gtagatgcgc cccagctcgc gcacctcctc gcggtggagc cgcctgaggc tggagcggtc
     2761 gagcatccgc agcaaatctt cgaggcgctg ccacgcgttc ttgcgttcgt cgatgaagcg
     2821 gtcggatgac acggcggtcg tgaactcgct ttgagaaagt cgtcgcgctt cgtgggcgcg
     2881 cgggttagaa tcttccgtgc ctcgcggtga tcgaagcgga agccggagat gaaaagaaag
     2941 tcagtcgcgg cgctctacga ccgcatcggc ggcaagtacg acgagaacaa gtcgcgcgcg
     3001 ataagcgact atacggaact gcccgccgtc ttgtcactgg ccggcgacgt gcgcggcctc
     3061 agcgtgctcg acgcggggtg cgggccgggc cgtcacgccg agaagctgct cgaccgcggc
     3121 gcgctcgtca ccggcatcga cgtgagcgag gagatggtcg ggctggcgcg cgagcgctgc
     3181 aaggggcgcg gcgagttcct gcgcgccgac ttcgagacgg cgcccttcgc gccctcgacg
     3241 ttcgacctcg tcacggcgag cctgtgcctg atgtattcga aggacgtgcg gcccgccatc
     3301 cgcaacttca gccggtggct gaagcatggc gggcggctcg tcttctcgct ctaccacccg
     3361 gttcgcttct tcgtgaagat accggacttc gatttctcga agagccgcaa ggtgtggatt
     3421 cacctcgaag ggtgcgacgt gacggtcttc aactactacc acccgctgcc gagatacttc
     3481 gacgcgctcg cggagagcgg cctcgaagtg ctgcgcctcg tcgagccgac gctgagccgc
     3541 cgtcacaagg gctggcccga agatttctac cgcatcccgc gctcgctcgt cgtcgcggcg
     3601 cgcaagcgct cgtgagcgcg gcgggccgca gggttgaccg aggcgacgac ttgaggcggc
     3661 gcggagactt gcatgggcga cgagttgaac gacagagaca tcgagttggt gcggcgcgcg
     3721 gtcgcgcgcg cgctcgaagc ggagcgcggc gggaacctcc ccatcggctc cgtgctggcc
     3781 gtcggcggcg aagtcgtcgc cgaggcgggc aacgccgtgc tcgtccccgt ctatcatccg
     3841 ggccgccacg ccgagaaaga ggcgctcgcg cgcgtgcccg cgtccctgtg gccgcgtgcc
     3901 cgcgagatga cctgctacac gacccttgag ccgtgcgtga tgtgcatggg cgcgctgctg
     3961 ctgcacggcg tcgggcgtgt cgtctacggc gcgcgcgacc cggagggcgg cggcggcgct
     4021 ctgctcgcgc acctgccgcg ctactacgcg ggcggcgcgg gcgtgccgct ctggctcggc
     4081 cccgcgctcg cggacgtttg cgacccgctc tacgaacgct cccgcgcccg cttcgacgcg
     4141 ctggcctgcg gcagagacaa cttctaagtc taagcatgaa cttctaagtc gaacgcatga
     4201 aatcattccg cggagaatcc ccttcgatga aactgacagc cgcgctcgtt ctgctcctgc
     4261 tcttctcgtc tcaggcgttc gcgcaaatgg acgagaccga ccgacgccgc aacgcgccgg
     4321 tcgagccgtt ccgcgtcatc gacaacgtct actacgtcgg cgcttcggac gtgaccgcgt
     4381 tcctcgtcac cacgccgcgg ggccacatcc tgatcgacgc gggctacgcc gagaccgcgc
     4441 cgcagatcaa ggccaacgtc gcgcggctcg gcttcaagat cgaggacgtg aaaatcctgc
     4501 tcaccacgca ggcgcacttc gaccacgcgg gcggtctggc cgagctgaag agggcgagcg
     4561 gggcgcgcct tctggcaagc cgggaagacg cggccctgat ggcgaacggc ggcaagggcg
     4621 acttcgcgtt cggcgaccgc tttccctacg aagcggtcaa gacagataag acgatagccg
     4681 acggcgagaa gatcggcctc gggggcgtca cgctgaaagc ggtctccacg cccggccaca
     4741 cgaagggctg cacggcctat acgacgacgg tgcgcgaggg cggcaaaaaa tacgaggtcg
     4801 tcttcttcgg gagcacgacc gcgcccggct acaagctcgt cggcaacgag cagtacccga
     4861 acctcgtcgc cgactacgaa cgcacgttcc gcgtctcgaa ggctctgaag ccggacgtgt
     4921 tcctcgcctc gcacggctct ttcttcgacc tcgcggggaa ggccgagcgc gcgcggcagg
     4981 gcgccgcgcc gaaccccttc atcgaccccg agagctaccg gcgctacatc ggagagaccg
     5041 agaaaacctt ccgcgagcag ctcctgaagg agacgcgagc gaagtgaagc ggccttgcct
     5101 ttttgttaac accgcgggcg atgcgtcatg ttaagatgag ccgtctctcg ctccatcccg
     5161 cgggcagtat tctttaaagt cattaaccct cgcgcaagag tttttcagtc agtcacgccc
     5221 gagccgtgtg cccgccgcgc cccgacggcc ccgcgggccg caggctccgc agacaaccgt
     5281 gaaccccctt tgacggacag gagaaaaaca tgcgttcccc ttcgatcagg aatctcgtgg
     5341 aatgtttcag gcgcggcggt ctcgcgctcg ttttcatctt cgcgctcgcg cccgcggcgg
     5401 cgctcgcaca gtcgcagatc acgaccggca ccgtccaggg caccatcgag gacgagaccg
     5461 gggcgagcgt gccgggcgcc aacgtcgagg ccaggaacgt ggagacggga ctcgcccgct
     5521 cgcagacgac cggcgacgac gggcgcttcg tcttcctgca actcgcgccc ggtcgctaca
     5581 cggtgacggt ctcgaagtcg ggcttcgcca ccatcgagca ggaggcgttc ccgctcaccg
     5641 tggggcagac cgtctcgctc gacctgcgga tgaaggtctc gggcgtggcc gagaaagtca
     5701 ccgtcagcgc ctcggacatc gccaccgtgg acacgacgaa gaccgaggcg agcacgacca
     5761 tcaaccgccg ctcggtcgag aac
//
LOCUS       DBNGOH010000922         3780 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1422814, whole genome shotgun sequence.
ACCESSION   DBNGOH010000922 DBNGOH010000000
VERSION     DBNGOH010000922.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3780)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3780)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3780
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1422814"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(42..869)
                     /locus_tag="AC159P_17980"
     CDS             complement(42..869)
                     /locus_tag="AC159P_17980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007277503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HIR3697387.1"
                     /translation="MSDAPDTRPAASDESTRPVSLVTGAGRGIGRGIAVELGRLGHAV
                     AVNYAGNTAAAEECLRLVREAGGDGLTVRADVSSSADRASLVNEVVGAYGRIDLLVNN
                     AGVAPDVRADLLEAGEESFDRLVGVNLKGPYFLTQLVARRMIEQVQQGNVNNAFGRPR
                     VVTVTSVSAYTASVNRGDYCVSKAGLAMMTKLYAARLAEHGINVYEIRPGVIATEMTG
                     GVREKYDRLIAEGAWPIRRWGEPSDVARAVASIARGDLPFSTGEVINVDGGFHLRTL"
     gene            complement(866..2143)
                     /locus_tag="AC159P_17985"
     CDS             complement(866..2143)
                     /locus_tag="AC159P_17985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012908998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HIR3697388.1"
                     /translation="MPTTMHGRGATRETLLEAFDFAQRQVRALVEKYPRDYYPMYTVG
                     GQYGQDRKRWTHWCDGFYPGMMFVFAEATGDEEWLDEAVRLATPLEERQYDRAVHDLG
                     FLFFSTYLRWLELGGPAERIEPVLVQAGRTMAMRFMERGQYLRSFVEPASLFIDIMMN
                     VGTIFHAAMETGDEHLLDIANRHCLTTRRTLVRGDGSTAHEAIFDLATGECLRQSTHQ
                     GYRGDSAWSRGLAWSLYGFTRSFLQSGRAEYLETAMLNADYYLEHTPPDGVTPWDYDA
                     PPAGPLARTQVDTSAAAIAASGLLDLADAVPDRTKARAYRDFAMTSLVSLSQKHLGSL
                     TPGFEGILNGGVYHIHKNLGVHEAVMFGEYFFVEALDKALSALDEEVVPPDLKPYPED
                     TLPAHARRWADGPTEEDMPAPSDEAHGGDGGKP"
     gene            complement(2258..3769)
                     /locus_tag="AC159P_17990"
     CDS             complement(2258..3769)
                     /locus_tag="AC159P_17990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HIR3697389.1"
                     /translation="MSQRLYTPPGTIRSVADFRVHFEKLGLSFPCDEEVAHGAQSPLA
                     QPITLGGRQIGNRFCIHPMEGWDGTLDGRPTEHTVRRWRHFGLSGAKLIWGGEAVAVR
                     HDGRANPNQLLLNEDTQRDLAVLRETLVAAHQEACSTTDDLFVGLQLTHSGRYSRPNE
                     KTRPEPRILYHHPILDRRVGVDASYPVLTDGEVREIIERYHDAARMSHELGYDFVDIK
                     HCHGYLGHEFLSAHTRGGDYGGSFENRTRFLREIVEGVRSVAPGMEIGVRLSAFDIVP
                     FRPDPARSTPGKLGPGIPEPYDNLLPYRWGFGVNAENPVEYDLAETFRFLSLLRELGI
                     RLVNVSVGSPYYNPHVQRPALYPPSDGYQPPEDPLVGVARQLEATRVLKEHFPDLVFV
                     GSGYTYLQEYLPNVAQAAVREGWVDSVGLGRMVLSYPDLPLDVVRGNALRRKLVCRTF
                     SDCTTAPRNGMISGCYPLDEHYKQLPEAVQLKRLKTAGRDAKEAKGAKPVGAK"
BASE COUNT          577 a         1202 c         1366 g          635 t
ORIGIN      
        1 ttcaatccgc aatccgcaat ccgcaatccg caatctttgt gtcagagcgt ccgcaggtgg
       61 aagccgccgt ccacgttgat gacttcgccg gtcgagaaag ggaggtcgcc gcgggcgatt
      121 gatgcgacgg cgcgggccac gtcggaaggc tcgccccagc ggcggatcgg ccacgcgccc
      181 tccgcgatca ggcggtcgta cttctctctg acgccgccgg tcatctccgt ggcgatgacg
      241 ccggggcgta tctcgtagac gttgatgccg tgctcggcga gccgcgccgc gtagagtttt
      301 gtcatcatcg cgaggcccgc tttcgagaca cagtagtcgc cgcggttgac cgaggcggtg
      361 taggcagaga cggaggtgac ggtgacgacg cgcggacgac cgaacgcgtt attgacgttg
      421 ccctgttgca cctgctcgat catgcggcgc gcgacgagct gcgtcaggaa ataaggcccc
      481 ttgaggttga cgcccacgag gcggtcgaac gactcttcgc ccgcttcgag caggtctgcg
      541 cgcacgtcgg gcgcgacgcc cgcgttgttg acgagcaggt cgatgcggcc atacgcgccg
      601 acgacttcgt tgaccagcga cgcgcggtct gccgacgagg acacgtcggc gcggacggtc
      661 agcccgtcgc cgcccgcttc gcgcacgagc cgcaggcact cttcggcggc ggcggtgttg
      721 cccgcgtagt tgacggcgac ggcgtggccg agccttccaa gctcgaccgc gatgccgcgc
      781 ccgatgccgc gccccgcgcc cgtcaccaga gagacgggcc gcgtgctctc atcgcttgcg
      841 gcggggcgcg tgtccggggc gtcgctcacg gcttgccccc gtcgccgccg tgcgcttcgt
      901 cggagggggc gggcatgtcc tcttcggtcg ggccgtcggc ccaccggcgc gcgtgcgcgg
      961 gcagtgtgtc ttcggggtag ggcttgaggt cgggcggcac gacctcttcg tcgagcgcgc
     1021 tgagcgcctt gtcgagcgcc tcgacgaaga agtattcgcc gaacatgacg gcctcgtgga
     1081 cgccgaggtt cttgtggatg tggtagacgc cgccgttgag gatgccctcg aagccgggcg
     1141 tgaggctgcc gagatgcttc tgcgaaagcg agacgagcga ggtcatggcg aagtcgcggt
     1201 aggcgcgcgc cttcgtgcgg tcgggcacgg cgtcggcgag atcgagcagg cccgaggcgg
     1261 cgatggccgc cgccgaggtg tccacctgcg tgcgcgcgag cggccccgcc ggaggcgcgt
     1321 cgtagtccca cggcgtcacg ccgtcgggcg gcgtgtgctc aaggtagtag tcggcgttga
     1381 gcatcgcggt ctccagatac tcggcccgcc cgctttgcag gaaggagcgc gtgaagccgt
     1441 agagcgacca cgcgagtccg cggctccaag cggagtcgcc gcggtagccc tgatgcgtcg
     1501 attggcgcag gcactcgccg gtcgcaagat cgaagatcgc ctcgtgcgcg gtcgagccgt
     1561 cgccgcgcac gagcgtgcgg cgcgtcgtca ggcagtggcg gttggcgatg tccaagagat
     1621 gttcgtcgcc ggtctccatc gcggcgtgga agatcgtgcc gacgttcatc atgatgtcga
     1681 tgaagagcga ggccggctcg acgaacgagc gcaggtactg gccgcgctcc atgaagcgca
     1741 tcgccatcgt gcggcccgcc tggacgagca ccggctcgat gcgctcggcg gggccgccga
     1801 gttcgagcca gcgcaggtag gtcgagaaga agaggaaacc gaggtcgtgc acggcgcggt
     1861 cgtactgccg ctcttcgagc ggggtggcta accggacggc ctcgtcgagc cactcctcgt
     1921 cgcccgtggc ctcggcgaag acgaacatca tgccggggta gaagccgtcg caccagtgcg
     1981 tccagcgctt acggtcctgc ccgtactggc cgccgaccgt gtacatcggg tagtagtcgc
     2041 gcgggtactt ctcgacaagg gcgcgcacct gtcgctgtgc gaagtcgaag gcttcgagca
     2101 gcgtctcgcg cgtcgcgccc cgcccgtgca tcgtcgtcgg catatctgga caatcctcca
     2161 atgaaaaaat gatgaatgat gaatgatgaa tgatgaaaga gaagcagcgt gccgccaatt
     2221 catcattcat cgttcatcat tcatcatttc gagaaactca cttcgcgccg accggcttcg
     2281 cgcccttcgc ttccttcgcg tcgcgccccg ccgtcttgag ccgtttgagc tgcacggcct
     2341 cgggcagttg cttgtagtgt tcgtcgagcg ggtagcagcc cgaaatcatg ccgttgcgcg
     2401 gcgcggtcgt gcagtcgctg aaggtgcggc agacgagctt gcgccggagg gcgttgccgc
     2461 gcaccacgtc gagcggcagg tccgggtacg agagcaccat ccgcccgagg ccgaccgaat
     2521 cgacccagcc ctctctgacg gcagcctgcg ccacgttcgg caggtactcc tgcaagtagg
     2581 tgtagcccga gccgacgaag acgaggtcgg ggaaatgctc cttgagcacg cgcgtcgctt
     2641 cgagctggcg cgcgacgccc accagcgggt cttcgggcgg ctggtagccg tccgacggcg
     2701 ggtagagcgc ggggcgctgg acgtgcgggt tgtagtaggg gcttccgacc gagacgttga
     2761 cgaggcggat gccgagttcg cggagcaaag agaggaagcg gaacgtctcc gcgaggtcgt
     2821 actcgaccgg gttctccgcg ttgacgccga agccccagcg gtagggcagc aggttgtcgt
     2881 aaggctcggg gatgccgggg ccgagtttgc cgggcgtcga gcgcgcgggg tcggggcgga
     2941 agggcacgat gtcgaaggcc gacagccgca cgccgatctc catgccgggc gccacgctac
     3001 gcacgccctc gacgatctcg cggaggaagc gcgtgcggtt ctcgaagctg ccgccgtagt
     3061 cgccgccgcg cgtgtgcgcg ctcaggaact cgtggccgag gtagccgtgg cagtgcttga
     3121 tgtccacgaa gtcgtagccg agttcgtgcg acatccgcgc cgcgtcgtgg tagcgctcga
     3181 tgatctctct gacctcgccg tcggttaaga ccgggtaact cgcgtccacg cccacgcggc
     3241 ggtcgaggat cgggtggtga tagaggatgc gcggctcggg ccgggtcttc tcgttcgggc
     3301 ggctgtagcg gcccgagtgc gtgagttgca acccgacaaa gaggtcgtcc gtcgtgctgc
     3361 acgcctcttg gtgcgccgcg acgagcgtct cgcgcaagac ggcgaggtcg cgctgcgtgt
     3421 cttcgttgag gagcagttgg ttcgggttcg cgcgcccgtc gtggcgcacc gcgacggcct
     3481 cgccgcccca gatgagcttg gcgccgctca ggccgaagtg gcgccagcgg cggacggtgt
     3541 gctcggtggg ccgcccgtcg agcgtgccgt cccagccctc catcgggtgg atgcagaagc
     3601 ggttgccgat ctgcctgccg cccagggtga tgggctgcgc gagcggcgat tgggcgccgt
     3661 gggcgacctc ctcgtcgcaa gggaacgaga gtcccagctt ctcgaagtga accctgaaat
     3721 ccgcgacgct acgaatggtt ccggggggcg tgtacaatct ctggctcatc aggcgaccga
//
LOCUS       DBNGOH010000923         2233 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4170770, whole genome shotgun sequence.
ACCESSION   DBNGOH010000923 DBNGOH010000000
VERSION     DBNGOH010000923.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2233)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2233)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2233
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4170770"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(33..998)
                     /locus_tag="AC159P_17995"
     CDS             complement(33..998)
                     /locus_tag="AC159P_17995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HIR3697390.1"
                     /translation="MRVGRDRIFLLLLASFISAPCAGALAQKATTGSDGGGSRRGGVR
                     SVTIPVTIKLPEKRQEEGELQYLENFTIFEDNEQQEVLAARGAPTYPLTLVVLVQDDL
                     DPTVGTEIAKLARFIRNLPQGSRVMVGYLRAGSLQVRQKFTSDLERAAKQLRVPTGSA
                     ALAPFNPFTLTRDAVKKFESQPVGRRAVLLISDGLDVSRGVENSTPTQSFDLQRSIDE
                     AQRRGAAVYSIYAPTTGGRSRTLAGNGQSSLERLSDETGGRAFIQTVSAPVVLEPHLR
                     EIEKLLSTQFALTYLSTHSNKGFHRIRIVTDLEGGEVRYPPGYTR"
     gene            complement(1170..1994)
                     /locus_tag="AC159P_18000"
     CDS             complement(1170..1994)
                     /locus_tag="AC159P_18000"
                     /inference="COORDINATES: protein motif:HMM:NF048886.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlkZ-related protein"
                     /protein_id="HIR3697391.1"
                     /translation="MSDSIPEEVEIFRDRRWRREPESRVETAVEAEALIETAGFCAAM
                     TDSRRPGPSLYVAVCGRRDAHMPRNVQKDPESSLTWLLKDEVMRRGRVYYAKLAKGRS
                     LFVAPRLVPAFNAVWGIARRKEAEALSADARAILKVLRREWEMATCDLRVASKVGERA
                     KFTRALDELQRTMKVIPQEVLYEPWFTYIWTLGEGRFDEQLSVKIKREDALREIARAF
                     LDGAGMTFRGELAKVTGLSRPDAGLGNRALVAEGYSERVGEGVYRLGDFDERLRGR"
BASE COUNT          341 a          796 c          708 g          388 t
ORIGIN      
        1 actgcctgct gcctgctgcc cgctgcctgc tgttatcgcg tgtagccggg cgggtagcgc
       61 acttcgccgc cttcgaggtc ggtgacgatg cggatgcggt ggaagccttt gttggagtgg
      121 gtcgagaggt atgtcagcgc gaactgcgtg gagaggagtt tttcgatctc gcgcaggtgc
      181 ggttcgagga cgacgggcgc gctgacggtc tggatgaagg cgcgaccgcc cgtctcgtcc
      241 gagagccgtt cgagcgaact ctgcccgttg ccggccagtg tgcggctgcg cccaccggtc
      301 gtcggcgcgt agatgctgta gacggcggcg ccgcgacgct gcgcctcgtc gatcgagcgc
      361 tggaggtcga agctctgcgt cggcgtcgag ttctccaccc cgcgcgacac gtcgaggccg
      421 tcgctgatga gcagcaccgc gcggcgcccc acgggctgcg actcgaactt cttgaccgcg
      481 tcgcgcgtga gggtgaacgg gttgaagggg gcgagcgccg ccgagccggt ggggacgcgc
      541 agttgcttgg ccgcgcgttc gaggtcggac gtgaacttct gccgcacctg tagcgaaccg
      601 gcgcgcaggt agcccaccat cacgcgcgac ccctgcggca ggttgcggat gaaccgcgcc
      661 agcttcgcga tctccgtccc gaccgtgggg tcgaggtcgt cctgcacgag cacgacgagc
      721 gtcagcgggt aggtcggcgc gccgcgcgcg gccagcacct cctgctgctc gttgtcctcg
      781 aagatggtga agttttcgag gtactgaagc tcgccctcct cctgcctctt ctccggcagc
      841 ttgatggtca cggggatcgt cacgctgcgg acgccgcccc gccggctccc gccgccgtcg
      901 ctcccggtcg tcgccttctg cgcgagggcg cccgcgcacg gcgccgagat gaaagacgcg
      961 aggagaagta gaaagatacg atcccgtcca actctcatca tgtttcctgt ggtgggccgt
     1021 ctaaaggatg aacggcgcgg acactgcaag cttgccgtgc gggcattata gcctgccgcc
     1081 gcgcggaagc gcaccgccac gttaaccttt tcgtccggcc atccgcgccg gcagcagcgg
     1141 ggagagtttc acttcggcct taagatacct tatcgcccgc gcagccgctc gtcgaaatcg
     1201 ccgaggcggt agacgccttc gcccacgcgc tcggagtagc cctcggcgac cagcgcgcgg
     1261 ttcccgagtc cggcgtcggg gcgcgacagc cccgtcacct tcgccagctc gccgcggaag
     1321 gtcatccccg ccccgtcgag gaacgcgcgc gcgatctctc tgagcgcgtc ttccctcttg
     1381 atcttgaccg agagctgctc gtcgaaccgc ccctcgccca gcgtccagat gtacgtgaac
     1441 cacggctcgt acagcacctc ctgcgggatc accttcatcg tccgctgtag ctcgtccaga
     1501 gcgcgcgtga acttcgcgcg ctcgcccacc ttcgacgcca cgcgcaaatc gcacgtcgcc
     1561 atctcccact cgcgccgcag caccttcagg atcgcacgcg cgtccgccga taaagcctcc
     1621 gcctcttttc tgcgcgcgat gccccacacc gcgttgaagg cggggacgag ccgcggcgcg
     1681 acgaacagcg agcgcccctt cgccagcttc gcgtagtaga cgcgcccgcg ccgcatcacc
     1741 tcgtccttga gcagccacgt cagactgctc tccgggtctt tctgcacgtt gcgcggcatg
     1801 tgcgcgtcgc gccgcccgca aaccgcgacg tagagcgagg ggccgggccg ccgcgagtcc
     1861 gtcatcgccg cgcagaaccc cgccgtctcg atcaaagcct ccgcctcgac cgccgtctcc
     1921 acacgcgact caggctccct gcgccaacgc ctgtcgcgga aaatctcgac ctcttcgggg
     1981 atggaatcgc tcatttatct tcgaatctca gctttgaact taaagcggca cagtgacggg
     2041 acaggccgcc gaggaattcg ccctcgcgat ggccgccgac ccttcaaaca cgtgagtatc
     2101 agttacacta ccggcgctcg attttcaatg cctcgcagct cttcatgccg ctgagccgcc
     2161 acgcacgccg agcgcggcgc ctacgtccgc gtccgggctt gaggaggacg acatccacgt
     2221 cgtctcacgc gac
//
LOCUS       DBNGOH010000924         2438 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1057481, whole genome shotgun sequence.
ACCESSION   DBNGOH010000924 DBNGOH010000000
VERSION     DBNGOH010000924.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2438)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2438)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2438
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1057481"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(72..2207)
                     /locus_tag="AC159P_18005"
     CDS             complement(72..2207)
                     /locus_tag="AC159P_18005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016944246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046559 - alpha-glucuronidase activity
                     [Evidence IEA];
                     GO_process: GO:0045493 - xylan catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucuronidase family glycosyl hydrolase"
                     /protein_id="HIR3697392.1"
                     /translation="MLFCVAVALQLCAGAEAVAEDGYRLWLRYDPLPRQSIQAYRPRV
                     ASVVAQGRSATFGAIRDELVNGCTGLLGFPVPSVEEVDRDGAVVVGTPDSSPLIKRLG
                     WQRQLAALGPEGFRLRSVKLAGRNVTVIASSGETGALYGAFHFLRLMQTLQPISNLNL
                     NEKPRLQLRVLNHWDNLDGSIERGYAGRSLWDWGALPDKVDPRLRDYARANASVGING
                     AVLNNVNANSKSLSAEYLRKAAAIADAFRPYGVRVYLSARFSAPIELGGLKTADPLAP
                     EVAAWWRAKADEIYTLIPDFGGFLVKASSEGQPGPHTYKRSHLEGANMLAAAVAPHKG
                     VVIWRAFVYDPKPGYDRAAAAYDELQRFDGQFAPNVLLQVKNGAIDFQPREPFHPLFG
                     AMPRTPMMLEAQITQEYLGFSNHLVFLAPMWREVLDSDTHARGPGSTVTRVLDGSLFG
                     QRVTGMAGVANTGTDRNWTGHHFAQSNWYAFGRLAWNPDAPSREIADEWIKMTLTRDR
                     AAAATIARLMLESHDALVDYMTPLGLHHIMWGGHHYGPAPWENKFERADWNPVYYHRA
                     DARGLGFDRSPEGSNAVGQYHAPVRARFGNPATTPEKYLLWFHHVPWDYRMRSGRTLW
                     DELATSYQRGVDWVRAARKDWDALSGVIDPERHAAVRQKLAIQERDAVHWKDAVLSYF
                     QTFSKRPLPAGVEPPRKTLEEYKATSLEW"
     gene            complement(2287..2433)
                     /locus_tag="AC159P_18010"
     CDS             complement(2287..2433)
                     /locus_tag="AC159P_18010"
                     /inference="COORDINATES: protein motif:HMM:NF037199.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5597 domain-containing protein"
                     /protein_id="HIR3697393.1"
                     /translation="MSAQEGKYVNGQWTPGRWLNGDQTHQGRHVRLPSGRFDIQRVKL
                     YRYR"
BASE COUNT          372 a          771 c          869 g          426 t
ORIGIN      
        1 ggcgattcgc taaaggtcta ttcaacttga gtgcaacgtg cctcgccttt caagatcgaa
       61 cgaagtacgg ctcaccattc aaggctcgtc gccttgtact cttcgagcgt cttccgcggc
      121 ggctcgacgc ccgcgggcag cggtcgcttc gagaaggtct ggaagtagga gagcactgcg
      181 tccttccagt gaacagcatc gcgttcttgt atcgcaagtt tctgcctcac ggcggcgtgc
      241 cgctcggggt cgatcacgcc ggagagcgcg tcccaatcct tgcgggcggc ccgcacccaa
      301 tccacgccgc gctggtagct cgtcgccagc tcgtcccaca gcgtccgccc cgagcgcatg
      361 cggtaatccc aaggcacgtg gtggaaccag agcagatatt tttcgggggt ggtggccggg
      421 ttgccgaagc gcgcgcgcac cggcgcgtga tactggccga cggcgttgct cccttcaggc
      481 gagcggtcga agccgaggcc gcgcgcgtcc gcgcgatgat aatagacggg gttccagtcg
      541 gcgcgctcga acttgttctc ccacggcgcg gggccgtagt ggtgcccgcc ccacatgatg
      601 tggtggaggc cgagcggcgt catgtaatcg accagcgcgt cgtgcgattc gagcatgagg
      661 cgggcgatgg tggccgccgc cgcgcggtcg cgcgtgagcg tcatctttat ccactcgtcc
      721 gcgatctcgc gcgaaggcgc gtcggggttc cacgcgagcc gaccgaaggc gtaccagttg
      781 ctttgcgcga agtggtggcc cgtccagttg cgatccgtac cggtgttggc gacgcccgcc
      841 atgccggtca cacgctggcc gaagagactt ccgtccagca ccctggtcac ggtcgagccg
      901 gggccgcgcg cgtgcgtgtc cgagtcgagc acttcgcgcc acatcggggc gaggaagacg
      961 aggtggttcg agaagccgag gtactcctgc gtgatctgcg cttcgagcat catcggcgtg
     1021 cgcggcatgg cgccgaagag cgggtggaaa ggctcgcgcg gctggaagtc gatggcgccg
     1081 ttcttgactt gcagcaggac gttcggcgcg aactggccgt cgaaccgctg tagctcgtcg
     1141 taggccgccg ccgcgcggtc gtagccgggc ttcgggtcgt agacgaacgc gcgccagatg
     1201 acgacgcctt tgtgcggcgc gaccgcggcg gccagcatgt tcgctccttc gaggtggctg
     1261 cgtttgtagg tgtgcggccc cggctggcct tcgctgctgg ccttgacgag gaagccgccg
     1321 aagtccggga tgagcgtgta aatctcgtcg gccttcgcgc gccaccacgc cgcgacttcg
     1381 ggggcgagcg ggtcggcggt cttcaggccg ccgagttcga tgggcgcgga gaagcgcgcc
     1441 gagaggtaga cgcgcacgcc gtaagggcgg aaggcgtcgg cgatggccgc ggccttgcgc
     1501 aggtactcgg cgcttaagct cttggagttg gcgttgacgt tgttgagcac cgcgccgttg
     1561 atgccgaccg acgcgttggc gcgcgcgtag tcgcgcaggc gcggatcgac tttgtcgggc
     1621 agcgcgcccc agtcccacag agagcggccc gcgtagccgc gctcgatgct gccgtcgagg
     1681 ttgtcccagt ggttgagcac gcgaagctgt agccgcggct tctcgtttag gttcaagtta
     1741 ctgatgggtt gaagcgtctg catcaagcgc aggaagtgga aggcgccgta gagcgcgccg
     1801 gtctcgccgc tcgaagcgat gaccgtgacg ttgcggcccg cgagtttgac ggagcggagg
     1861 cggaagcctt cggggccgag cgcggcgagt tgtcgttgcc agccgagccg tttgatgaga
     1921 ggcgagctgt cgggagtccc gacgaccact gcgccgtcgc ggtcaacctc ttcgacggac
     1981 ggcacgggga agccgagcag gcccgtgcag ccgttgacga gttcgtcgcg gatggcgccg
     2041 aaggtcgcgg agcggccctg cgccaccacg gaggcgacgc ggggccggta cgcttgaatc
     2101 gactgccgcg gcagcgggtc gtaacgcagc cacaggcggt agccgtcctc ggccacagcc
     2161 tccgcgcccg cgcacaactg aagcgcgacg gcaacgcaga acaacatcgc cgtctcgatt
     2221 cgccgtcgct gcggacacgt ccgcttcatt tcggtcacgg tggctttcat caatcgcgta
     2281 tggccctcag cggtagcggt agagcttgac gcgctggatg tcgaacctgc cggaaggcag
     2341 gcgcacgtgg cggccctgat gcgtctggtc gccgttcagc cagcggcccg gcgtccactg
     2401 cccgttgaca tacttccctt cctgcgcgga catgatgc
//
LOCUS       DBNGOH010000925        13459 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3805194, whole genome shotgun sequence.
ACCESSION   DBNGOH010000925 DBNGOH010000000
VERSION     DBNGOH010000925.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 13459)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13459)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13459
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3805194"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(86..1321)
                     /locus_tag="AC159P_18015"
     CDS             complement(86..1321)
                     /locus_tag="AC159P_18015"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020154361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR3697394.1"
                     /translation="MIDFELTEDHKAIRQTVRDFARAEVAPRIKELDEQQRFDRSILD
                     KMAELNLLGVCIPEEYGGAGFDYISLGLVCEELEAVDTFMRVIMSVHTGLNSMTLLTW
                     GTEEQKRKYLVPQAKGEKIATYGLTEPGAGSDVVGARSTARREGDEWVLNGEKMWISL
                     AGDVADNFLFFCWTDEEKRRQRDHTGMSCFIVERSMPGFSSGTIHGKLGIRAGNTGYF
                     SLQDVRVPAENLVGEEGEGFKIAMFALEQGRYTVASGATGIIRASRDASVAYALERET
                     FGTKIANHQLVKQKIAEMESDYQMCHLLWLRAGYLKNEGKPNARATSLAKWQATVRSE
                     KAASMAIEVHGANGYSNDYPVERYLRNCKAAVIYEGTRDIHTLMQADWALGLKSEKPA
                     RKTLPPYRPTESRDTVAAD"
     gene            complement(1520..2917)
                     /locus_tag="AC159P_18020"
     CDS             complement(1520..2917)
                     /locus_tag="AC159P_18020"
                     /inference="COORDINATES: protein motif:HMM:NF014069.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0052929 - ATP:3'-cytidine-cytidine-tRNA
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HIR3697395.1"
                     /translation="MPTRVPEKIMRLARAVRDAGGRALMVGGCVRDRLMGRAAKDWDV
                     EVYGVEPARLRAILDRLGRVNVVGEAFTVYKLGHDLDVSLPRRERKTGRGHRGFVIEG
                     DPAMPVAEAARRRDFTVNAILEDPLDGDLLDPFGGRADIERGLIRAVAPETFVEDSLR
                     VLRAAQFAARFEFEIEPATVELCRSIDLTDLPPERVWGELEKLLLGARRPSIGFEWLD
                     RLGATAQLFPEVEALKGVEQEPDWHPEGDVLTHTLLVLDRARELVDDLDYARRVTVML
                     AALCHDFGKPATTEFIEGRIRSRGHEEAGVAPTEKFLDRLRVFTLEGYDVRAQVLALV
                     RDHLKPGELYKRREEVGDGAFRRLARRCELELLYRVAKADSLGRNAPWVPREKWFTAD
                     AQEWFIARARELSVATGPPPPLLMGRHLIELGLTPSPQFKKILDAVYELQLDNRVLTL
                     EEAREEARKLMMNAE"
     gene            complement(2959..3471)
                     /locus_tag="AC159P_18025"
     CDS             complement(2959..3471)
                     /locus_tag="AC159P_18025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697396.1"
                     /translation="MPVVKKRNQQTETRLLLEGDDPVELPPAEVADSAVVLQFETFRQ
                     YVPELLEELRDGTVVEPVPVMLDGSTFVVHGETRSFWVKLWHSSPERTRVERVQIISC
                     LPSMRGVQILVDDLEGYEDDEDFDDDESDFVEEAHVEDDDELEEDEDDKPGGGSTEDS
                     NGGSRGGSPR"
     gene            complement(3570..4997)
                     /locus_tag="AC159P_18030"
     CDS             complement(3570..4997)
                     /locus_tag="AC159P_18030"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020666193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HIR3697397.1"
                     /translation="MAAPNIETIVSLSKRRGFVFPSSEIYGGLGSAWDYGPLGTELTT
                     NIKRVWWRWMVYERDDMEGLDSSIILNRLVWKHCGHEATFNDMLTDCRECKSRWRADK
                     LDFPREYGSASNALAAGVVKCPNCGSKDLTEPRPFLTMLRTRLGAAADEEDYLSLAYL
                     RPETAQGIFINFLNVQQSMRRKLPFGIAQVGKSFRNEVTPGNFIFRTREFEQMEMEYF
                     VRPGEDEELHQYWIDACRDWYHGLGLSATNLRLYEQPKEELAHYAKRTVDIQYRFFPE
                     REEDERQWDELMGIANRTDFDLKAHSNKPADEEGKRVNPDSTEDLSYFDEGSKQRFYP
                     YVIEPAAGVNRTMLAVMMDAYTEKTTDKGEKRVVLKLHPELAPIKVAVLPLAKNKPEI
                     VRMARGLKSQLQPSMRAVYDDTGGIGKLYARQDEIGTPFCVTVDHQSLEDEAVTVRDR
                     DTWEQERVKVSELQEHLKRRLGIKC"
     gene            complement(5272..5403)
                     /locus_tag="AC159P_18035"
     CDS             complement(5272..5403)
                     /locus_tag="AC159P_18035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697398.1"
                     /translation="MAILVIFLVVVVLAFLAWQGGMFGGSKTTKIDVDVKAPAAPSK"
     gene            complement(5722..6789)
                     /locus_tag="AC159P_18040"
     CDS             complement(5722..6789)
                     /locus_tag="AC159P_18040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase associated/transthyretin-like
                     domain-containing protein"
                     /protein_id="HIR3697399.1"
                     /translation="MPHSPRTRRAPARLVARLVALAALFAVVFAVAPAPDSEARAKQK
                     NKKNQPTTGRIEVSTNPGGYPILIDGRPAGETTDYVRAIDLEPGTHTVEIIFPNNTRW
                     SHVFNIVAGRKDCIALNYRPRTISIPAVPTSPCPYPVNVSAPASVNDGDVITFSADVG
                     YAGPSALNYAWTVSPPTARILTGAGTPTITVDSTGLGSQRVTAVLVVDDGSGDRSCRQ
                     TAQAQTGILVQYIRQTPRRFDEFPSVAFDDDKARLDNLAVELQNNPTAVGYIIAYAGR
                     RSRPGHADRMGTRAADYLVNTRGINRNRLVVVNGGTRENNTYELWLVPQGAEPPRPTP
                     TVSADELRTAPSTARRPARRG"
     gene            complement(6793..7452)
                     /locus_tag="AC159P_18045"
     CDS             complement(6793..7452)
                     /locus_tag="AC159P_18045"
                     /inference="COORDINATES: protein motif:HMM:NF019225.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HIR3697400.1"
                     /translation="MKHQALRPAALMLSFLLLAAVPAKAGTVHFADAVAPSQGRTGAA
                     LDVRLRIAPQSGNVATAAQQTGRRGGPASSPQQTGTNTSGLPAGTAAPADTTLTQSGG
                     QVEVVEFGDVTGTVCDCGEIPPAIIPGGGFPLWPLLGLAGVPLAFLPGGNDRTPPGFN
                     PPPPVPTPPPPTPPSTPVPEPATLLLFGTSLLALGAGARRRLARRREGEGAAVAAEEV
                     F"
     gene            complement(8002..10149)
                     /gene="pnp"
                     /locus_tag="AC159P_18050"
     CDS             complement(8002..10149)
                     /gene="pnp"
                     /locus_tag="AC159P_18050"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HIR3697401.1"
                     /translation="MAVKKYLKEKIKLGDKELTVETGRIAKQADGSVVIGYGETMLLV
                     AAVGADKPREGIDFFPLTVEYREHNYAAGRIPGNYFRREGRPNEKEVLTCRLIDRPIR
                     PLFAEGYRNETQVIASVISADDENDPDVIAITGASLALYLSDIPFTTPIAGVRVGLIE
                     GRYVINPTYDQVRESSLNLIVAGTEEAIVMVEAGAKEVSEEIMVEALMLAHKEINRLC
                     RWQKELYKALEIEKRAVEPPVLNEEMMGEIERNYTDRLRASLDTSEQGKGASYAAVDA
                     LKKEVVEAYPEDQPEQRLMAKKIFDHIKEKVFRDDILNNRRRPDGRRFSEIRPIDCEV
                     SWLPRVHGSALFTRGETQAIVTTTLGTKEDEQFMDDLEAGEIKRRFLLHYNFPHYSVG
                     EVGRFGSTSRREIGHGALARRALEPILPAEADFPYSLRIVSDITESNGSSSMASVCGG
                     SLSMMDAGVPIRAAVAGVAMGLVMEGNKYAILSDIAGAEDHYGDMDFKVAGTRDGITA
                     LQMDIKIAGINAQIMAEALQQAKKGRLHILGVMEEALGEPRADLSPHAPRIITIKINP
                     DRIRDVIGPGGKMIRSIVEETGAKIDVEDDGTVFIATADGDAAQAAVARIRGLTAEAE
                     IGETYLGTVSRIVDFGAFVEIFPGTDGLLHISEIAEHRVRDVRDELKEGQQILVKCIG
                     KEGNKIKLSRKAVLRDEKKNDKPKADAAHGGDE"
     gene            complement(10535..10804)
                     /gene="rpsO"
                     /locus_tag="AC159P_18055"
     CDS             complement(10535..10804)
                     /gene="rpsO"
                     /locus_tag="AC159P_18055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HIR3697402.1"
                     /translation="MALTAESKETIIGQYRTHEGDTGSPQVQVALLTQRINELTEHFK
                     THKKDNHSRRGLLKMVSQRRSLLDYLKRRDIEGYHELIGKLGLRR"
     gene            11239..12486
                     /locus_tag="AC159P_18060"
     CDS             11239..12486
                     /locus_tag="AC159P_18060"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003954 - NADH dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006116 - obsolete NADH oxidation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR3697403.1"
                     /translation="MDASEERDPQPARIVVIGGGFGGLFTALGMARAGEVTLITNEDH
                     FLHTPMLYEYLSGEVEAWHIAPTYRELLGDDRVRFVQGAVVNVDFDAKEVEVAGRVRR
                     LAYDYLVLAVGGVTNFWGVEGAEKYAMPFRKLRHADDLRARMIESLDRVQPDAAPQDA
                     RRAATFVVVGAGASGVELATKMSDLLTDAFRRRGLRGEPRVVVVEMGDEVVPGMADDI
                     RRVVEEALRERRIELHTRARVVKVSEDGAVIEHEGERTEIEASGVIWTAGVRVNPLVE
                     KLNVERDRRGLIVVEPSLQVRGREGVFAVGDIALFTDIPPSLAGTAQLAFQESSLVAA
                     NVQALIDGGVPARGHFQELGEAVSLGTADAAVLAAGQVVTGPLARQARFALYTTRLPT
                     WQHRLKVGAAWFFGGKTPRPLGL"
     gene            complement(12583..13332)
                     /locus_tag="AC159P_18065"
     CDS             complement(12583..13332)
                     /locus_tag="AC159P_18065"
                     /inference="COORDINATES: protein motif:HMM:NF013625.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HIR3697404.1"
                     /translation="MPAIKIQKSVGLNAVNRAADVLAVKTRLIELGFDWLTADEVMGP
                     TTVKAIKLFQAIKNGRNQVNIQGNDGRIDPGGDTLKWLNAENAPRWMQMPPGSPAEGF
                     HNHELTQTNDAHDFGTSWLADTLKAAAASYKEEHLSANTNAALLTLNDFSLPQGGDTP
                     MHATHESGLACDIRLPRKNGQTGGGVVGQNAYDRKAMRAMILAFRRQPLASRVLLSDQ
                     VLVAEGLCMAAQGHANHAHFEVKPPARIDPD"
BASE COUNT         2062 a         4279 c         4647 g         2471 t
ORIGIN      
        1 ggcgaaagaa cagtagccca cggcgcgcgc cgtgggctac tgttctttcg cccgctccgc
       61 gggcttcctt ctcgggccct cggatttaat cggcggcgac cgtgtcgcgc gactccgtcg
      121 ggcggtaggg cgggagcgtc ttgcgcgcgg gcttctcgct tttcagcccg agcgcccagt
      181 cggcctgcat cagcgtgtgg atgtcgcgcg tgccctcgta gatgacggcg gccttgcagt
      241 tgcgcaggta gcgctcgacc gggtagtcgt tcgagtagcc gttcgcgccg tgcacctcga
      301 tcgccatcga cgcggccttc tcgctacgga cggtggcctg ccacttggcg agcgaggtcg
      361 cgcgcgcgtt cggcttgccc tcgttcttca ggtagcccgc gcgcagccac agcaggtgac
      421 acatctggta gtcggactcc atctcggcga tcttctgttt gacgagctgg tggttggcga
      481 tcttggtgcc gaacgtctcg cgctcgagcg cgtacgcgac ggacgcgtcg cgcgaggcgc
      541 ggatgatgcc ggtcgcgccc gaggcgacgg tgtagcgccc ctgctccagc gcgaacatgg
      601 cgatcttgaa gccctcgccc tcttcgccga ccagattctc ggcgggcacg cgcacgtcct
      661 gcaaagagaa gtagccggtg ttgcccgcgc ggatgccgag cttgccgtgg atggtgccgg
      721 aagagaaacc gggcatcgag cgctcgacga tgaagcagga catgcccgtg tggtcgcgct
      781 ggcgtcgctt ctcctcgtcc gtccagcaga agaagaggaa gttgtcggcc acgtcgcccg
      841 ccaaagatat ccacatcttc tcgccgttca gaacccactc gtcgccctcg cgccgcgcgg
      901 tcgaacgagc gcccaccacg tccgagccgg cgcccggctc ggtcaatcca taggtcgcga
      961 tcttctcgcc cttcgcctgc gggacgagat acttgcgctt ctgctcctcg gtgccccacg
     1021 tcagcagcgt catcgagttc aggcccgtgt ggacgctcat gatgacgcgc atgaaggtgt
     1081 ccacggcttc gagttcctcg cagacgaggc cgagcgagat gtagtcgaag cccgccccgc
     1141 cgtactcctc cggaatgcag acgcccagga ggttcagctc ggccatcttg tcgaggatgg
     1201 agcggtcgaa ccgctgctgc tcgtcgagtt ctttgatgcg cggcgccacc tccgcccgcg
     1261 cgaagtcgcg cacggtctgg cggatcgcct tgtggtcttc ggtcagttcg aaatcgatca
     1321 tgacgattct ccgtagttga attcaggctc gcacatggag acgaccgcgc gaggcggaag
     1381 agccgaatcc gatgcgtgtg ttcgtggtag aaagtgcgaa aaagttaagc ggcatcttac
     1441 accatgaggg ggaaaaaggg gaaaaggcga agaggcgaaa aggaggacga caagcagcgt
     1501 gcttctcatt catcgttcat cattccgcat tcatcattaa tttccgtgcc tcttcccttg
     1561 cctcttcgag cgtgagcacg cggttgtcga gttgcagctc gtagacggcg tcgagaatct
     1621 tcttgaactg cggcgagggc gtgaggccca gctcgatgag gtggcggccc atgaggaggg
     1681 gcgggggcgg gccggtcgcg accgagagtt cgcgcgcgcg cgcgatgaac cactcctgcg
     1741 cgtccgccgt gaaccacttc tcgcggggca cccacggcgc gttgcgcccc agcgagtcgg
     1801 ccttcgccac gcggtacagc agctccagct cgcagcgccg cgcgaggcgg cggaaggcgc
     1861 cgtcgccgac ctcttcgcgc cgcttgtaca gctcgcccgg cttcaggtgg tcgcgcacga
     1921 gcgcgaggac ttgcgcgcgc acgtcgtagc cttcgagcgt gaacacgcgc aggcggtcga
     1981 ggaatttttc ggtcggcgcg acgcccgcct cctcgtggcc gcgcgagcgg atgcgcccct
     2041 ctatgaactc ggtcgtcgcg ggcttgccga agtcgtgaca gagcgccgcc agcatcaccg
     2101 tcacgcgccg cgcgtagtcg aggtcgtcaa caagttcgcg cgcgcggtcg aggacgagca
     2161 aggtatgcgt taacacatcc ccctcggggt gccaatccgg ttcttgctcg acgcccttga
     2221 gggcttcgac ttcggggaag agctgcgcgg tcgcgccgag ccggtcgagc cactcgaagc
     2281 ctatggaggg gcggcgcgcg ccgagcagga gtttctccaa ctcgccccag acgcgctcgg
     2341 gcggcaagtc cgtgaggtcg atggagcggc acagctcgac cgttgcgggt tcgatctcga
     2401 actcgaagcg ggcggcgaac tgcgcggcgc gcaggacgcg caacgagtct tcgacgaagg
     2461 tctcgggggc gacggcgcgg atgagtccgc gttcgatgtc ggcgcgaccg ccgaaggggt
     2521 cgagcaggtc gccgtcgagc gggtcttcga ggatggcgtt gacggtgaag tcgcggcggc
     2581 gcgcggcctc cgcgacgggc atggcggggt cgccctcgat gacgaagccg cggtggccgc
     2641 ggcccgtctt gcgctcgcgg cgcgggagcg aaacgtcgag gtcgtggccg agcttgtaga
     2701 cggtgaacgc ctcgccgacg acgttgacgc ggcccaggcg gtcgaggatc gcgcgcagcc
     2761 gcgcaggctc gacgccgtag acttccacgt cccagtcctt cgccgcgcgc cccatcaggc
     2821 ggtcgcgcac gcagccgccg accatcagcg cgcgcccgcc cgcgtcgcgc accgcgcgtg
     2881 cgagccgcat gattttttcg ggaacgcgag tcggcatcag ccgaatctta tgtgagcggc
     2941 ggggaggaac acaaagccct atcgcggaga cccgccgcgc gacccgccgt tcgagtcctc
     3001 ggtcgagcca ccgccgggtt tgtcgtcttc gtcttcttca agctcgtcgt cgtcctcgac
     3061 gtgcgcctcc tcgacgaagt cgctttcgtc gtcgtcgaag tcttcgtcgt cctcgtagcc
     3121 ttccagatca tccacgagaa tctgcacgcc gcgcatcgag ggcaggcaac tgatgatctg
     3181 cacgcgctcg acgcgcgtgc gctcgggcga agagtgccag agcttgaccc agaagctgcg
     3241 cgtctcgccg tggacgacga aggtcgagcc gtcgagcatc accggcacag gctcgaccac
     3301 agtcccgtcg cgcagctctt cgagcaactc gggcacgtac tggcggaacg tctcgaattg
     3361 aaggacgacc gccgaatccg caacctccgc gggcggcagc tccacggggt cgtcaccttc
     3421 gagcaggaga cgcgtctcgg tctgctggtt tctttttttg acgacaggca taagttagta
     3481 aatggtcaat ggtgaatagt gaatggtgaa tggtgaacag gaagacccgt tttctccatt
     3541 caccattgac cattcactat tcacttgcct cagcacttga tgccgagcct ccgtttgagg
     3601 tgctcttgca gctcggagac tttcacgcgc tcctgctccc acgtgtcgcg gtcgcgcacg
     3661 gtgacggcct cgtcttcgag cgactggtga tcgacggtga cgcagaaggg cgtgccgatc
     3721 tcgtcctgcc gcgcgtagag cttgccgatg ccgcccgtgt cgtcgtagac ggcgcgcatc
     3781 gagggctgga gctgcgactt gaggccgcgc gccatccgga cgatctcggg cttgttcttg
     3841 gcgagcggga gcacggcgac cttgatgggc gcgagttccg ggtgcagttt caggacgacg
     3901 cgcttctcgc ccttgtcggt cgtcttctcg gtgtaggcgt ccatcatcac ggcgagcatc
     3961 gtgcggttga cgcccgccgc cggctcgatg acgtaagggt agaagcgctg cttgctgccc
     4021 tcgtcgaagt acgagaggtc ttccgtcgag tcggggttga cgcgcttccc ttcctcgtcc
     4081 gcgggcttgt tcgagtgggc cttcaagtcg aagtccgtgc ggttggcgat gcccatcagc
     4141 tcgtcccact gcctctcgtc ctcctcgcgc tcggggaaga agcggtactg aatgtccacg
     4201 gtgcgcttgg cgtagtgcgc cagctcctcc ttcggctgct cgtagagccg caggttcgtg
     4261 gccgagaggc cgaggccgtg ataccagtcg cggcaggcgt cgatccagta ctgatgaagc
     4321 tcttcgtcct cgccggggcg gacgaagtat tccatctcca tctgctcgaa ctcgcgcgtg
     4381 cggaagatga agttgccggg cgtcacctcg ttgcggaacg acttgccgac ctgcgcgatg
     4441 ccgaacggga gcttgcgccg catcgactgc tggacgttca ggaagttgat gaagatgccc
     4501 tgcgccgtct cgggccgcag gtacgcgagc gagaggtagt cctcctcgtc cgccgcggcc
     4561 cccaatctcg tgcggagcat cgtcaggaac gggcgcggct cggtcaaatc tttcgagccg
     4621 cagttggggc acttcaccac gcccgcggcg agggcgttcg aggcgctgcc gtactcgcgc
     4681 gggaagtcga gcttgtccgc ccgccagcgg ctcttgcact cgcggcagtc ggtgagcatg
     4741 tcgttgaagg tcgcctcgtg gccgcagtgc ttccacacga ggcggttgag gatgatggac
     4801 gagtccaggc cctccatgtc gtcgcgctcg tagaccatcc agcgccacca cacgcgcttg
     4861 atgttcgtcg tcagctccgt ccccagcggg ccgtagtccc acgcgctccc cagcccgccg
     4921 tagatttcgg acgaggggaa gacgaagccg cggcgcttcg agagcgagac gatggtttcg
     4981 atgttcggtg cagccacttg tactctccca aaagccgtga accgtaaacc gtaaaccgtg
     5041 acgagaagaa agcagtgacg agtaccacgt gacgagcgac gggaagaaag cagttcttca
     5101 ctcgtcacgg ttcccggctt acggtttacg tctttcgagg ataacagtcg cgcgccggaa
     5161 ttgaaaagaa gggaggggcg gcggcgcatg gtgcgtgccg tcgcccctct tcggacagga
     5221 acggactcgt tggttgttaa agccgggcgg cgaggtcgcc gtagcgcgcc gctacttgga
     5281 gggcgccgcc ggcgccttca cgtcaacgtc gatcttggtc gtcttgctgc cgccgaacat
     5341 gccgccctgc cacgccagga aggcgagcac gaccaccacc aagaagatta cgaggattgc
     5401 caccacgccg gtattgcttg agctttcgcc gtcacccatt tctttccatc ctcctagaga
     5461 ttcatacgtg tcgggtgcgc cctcttcgga cgctacgcaa attcggaagt cttaaccacc
     5521 cgccgccaaa aagaacaagc ggccccctgc tcgtttgata acagtacgcg ggattaacgt
     5581 cccgagggtg tttaagaaaa agccgtgcca cagggaaagg ggaagggtaa gtggggccga
     5641 aaaccgaaga ggacgagagg cacggtgctt ctcgtcctct tcggtttcgg agttcgtccg
     5701 ccttccgcgt ctcgtcccgt ttcagccgcg gcgggcgggg cggcgcgcgg tcgaaggcgc
     5761 cgtgcggagt tcgtccgcgc tgacggtcgg cgtcgggcgc ggcggctcgg cgccctgcgg
     5821 gacgagccac agttcgtagg tgttgttttc gcgcgtgccg ccgttgacga cgacgaggcg
     5881 gttgcggttg atgccgcgcg tgttgacgag gtagtcggcg gcgcgcgtgc ccatgcggtc
     5941 ggcgtggccg gggcggctgc ggcggcccgc gtaggcgatg atgtagccga cggcggtggg
     6001 gttgttctgg agttcgacgg cgaggttgtc gagccgcgcc ttgtcgtcgt cgaaggcgac
     6061 ggacgggaac tcgtcgaagc gccgcggggt ctgcctgatg tactgcacga ggatgccggt
     6121 ctgcgcctgc gcggtctgcc ggcaactgcg gtcgccggag ccgtcgtcca cgacgaggac
     6181 ggcggtgacg cgctggctgc cgaggccggt cgaatcgacg gtgatggtcg gcgtgcccgc
     6241 gcccgtcagg atgcgcgccg tgggcgggct gaccgtccac gcgtagttga gcgcggaggg
     6301 gccggcgtag cccacgtccg ccgagaaggt aatcacgtcg ccgtcgttga ccgaggcggg
     6361 cgccgagacg ttgacggggt acgggcaggg cgaagtcggg accgccggga tgctgatggt
     6421 gcgcgggcgg tagttgagcg cgatgcagtc cttgcgcccg gcgacgatgt tgaagacgtg
     6481 cgaccagcgc gtgttgttcg ggaagatgat ctcgacggtg tgcgtgccgg gttcgaggtc
     6541 gatggcgcgg acgtagtcgg tcgtctcgcc ggcggggcgc ccgtcgatga ggatcgggta
     6601 gccgccgggg ttggtcgaga cctcgatgcg gcccgtggtc ggctggttct tcttgttctt
     6661 ctgcttggcg cgcgcctcgg agtcgggcgc gggcgcgacg gcgaagacga cggcgaagag
     6721 ggcggcgagt gcgacgagtc gcgcgacgag cctcgcgggg gcgcggcggg tgcggggcga
     6781 gtgcggcatg ggctagaaga cctcctctgc ggcgacggcg gcgccttcgc cttcacggcg
     6841 gcgcgcgagg cggcggcgcg ctccggcgcc gagcgcgagc agactcgtgc cgaacaggag
     6901 cagcgtggcc ggctcgggaa ccggcgtgga gggcggcgtc ggcggcggcg gcgtcgggac
     6961 gggcggcggc gggttgaacc ccggcggggt tcggtcgttg ccgccgggga ggaacgccag
     7021 cgggaccccg gccagcccca gcaggggcca cagcgggaag ccgccgccgg ggatgatcgc
     7081 ggggggtatc tcgccgcagt cgcagaccgt gccggtcacg tcgccgaact cgacgacctc
     7141 gacctgcccg ccgctctgcg tgagcgtcgt gtcggcgggc gccgcggtgc ccgccgggag
     7201 gccggaagtg ttcgtgccgg tctgctgcgg ggaggacgcg gggccgcccc tccggcccgt
     7261 ctgctgcgcc gcggtcgcga cgttgccgct ctgcggcgcg atgcgcagcc gcacgtccag
     7321 agccgcgccc gtccgccctt gagagggcgc gaccgcgtcg gcgaagtgga cggtgccggc
     7381 cttcgcgggc acagccgcca ggagcaggaa gctgagcatc agagcagcgg ggcgtaacgc
     7441 ttgatgcttc attaaggggt cgtcctcctt gaattccctc gccgccggag actgtcggca
     7501 catcacgggc gaaccgtcaa aaggaatcct ggcactatcg atgaaaccaa tgcttcggag
     7561 acgcgggcgg cgacttcgaa ccgaacccgt tgcgggcgtt tcaaagtccg tgcttctcat
     7621 ccgccgtcag ggacggcgac ccctgtctct tctgatttcc cacaatctca aggactcgcg
     7681 gggaaaaccg cgatccggtt ttctgtacta agaaaagacg ctgaataacg tttgttttcc
     7741 agtttcaggt tgcacaatta aactccccgc cgcaagctgt gtcaagataa aacaggcccg
     7801 ccccccgcgg gcgaaccctt ttcctgacaa aaaattagac gcggcggacg aaaggcaaag
     7861 gcgaagcggg gaaaagggga aaaggggaag aggaagacgg ggcgaaaagg cgaaggggcg
     7921 aagagggagg atgacaagca cgctgcttct cgtcttccct cttcgcccct tcgccttttg
     7981 gccttttggc ccccgccttt cctactcgtc gccgccgtgg gcggcgtcgg ccttgggctt
     8041 gtcgttcttc ttctcgtcgc ggagcacggc cttgcgcgag agcttgatct tgttgccctc
     8101 tttgccgatg cacttgacga gaatctgctg cccctccttc agctcgtcgc gcacgtcgcg
     8161 gacgcggtgc tcggcgatct cggagatgtg cagcaggccg tccgtgccgg ggaagatttc
     8221 gacgaaggcg ccaaaatcga cgatgcgcga gacggtgccg aggtaggtct cgccgatctc
     8281 ggcctcggcg gtcaggcccc ggatgcgcgc gaccgccgcc tgcgccgcgt cgccgtcggc
     8341 ggtggcgatg aagaccgtgc cgtcgtcctc cacgtcgatc ttggcgccgg tctcctcgac
     8401 gatggagcgg atcatcttgc cgcccggccc gatcacgtcg cggatgcggt cggggttgat
     8461 cttgatggtg atgatgcgcg gcgcgtgcgg cgagaggtct gcgcgcggct cgccgagcgc
     8521 ctcctccatc acgccgagga tgtgcaggcg ccccttcttc gcctgctgca acgcctcggc
     8581 catgatctgc gcgttgatgc ccgcgatctt gatgtccatc tgcaaggcgg tgatgccgtc
     8641 gcgcgtgccc gccaccttga agtccatgtc gccgtagtgg tcttcggcgc ccgcgatgtc
     8701 ggagaggatg gcgtacttgt tgccctccat gacgaggccc atcgccacgc ccgcgaccgc
     8761 cgcccggatc ggcacgcccg cgtccatcat cgagagcgag ccgccgcaca ccgaagccat
     8821 cgaactcgac ccgttcgact ccgtgatgtc cgagacgatg cggagcgagt aggggaagtc
     8881 ggcctcggcg ggcaggatgg gttcgagcgc gcggcgcgcg agggcgccgt ggccgatctc
     8941 gcggcggctc gtggagccga agcgcccgac ctcgccgacc gagtagtgcg ggaagttgta
     9001 gtgcagcagg aagcggcgct tgatctcgcc cgcttcaagg tcgtccatga actgctcgtc
     9061 ctctttcgtg ccgagcgtcg tggtgacgat ggcctgcgtc tcgccgcgcg tgaacaaggc
     9121 ggaaccgtgg acgcgcggga gccaactgac ttcgcagtcg atggggcgaa tctctgagaa
     9181 gcggcgaccg tcggggcggc ggcggttgtt gaggatgtcg tcgcggaaaa ccttctcctt
     9241 gatgtggtcg aagattttct tcgccatcaa acgctgctcg ggctggtctt cggggtaagc
     9301 ctcgacgacc tctttcttca aggcatcgac ggcggcgtag gaagcgccct tgccctgctc
     9361 ggaagtgtcg agcgaggcgc gcaagcggtc tgtgtagttg cgctcgatct cgcccatcat
     9421 ctcctcgttc aggacgggcg gctcgaccgc gcgcttctcg atttcgagcg ccttgtacag
     9481 ctccttctgc cagcggcaga ggcggttgat ctccttgtgc gcgagcatca gggcctcgac
     9541 catgatctcc tcggagacct ccttcgcccc ggcctcgacc atgacgatgg cctcttcggt
     9601 gcccgcgaca atgaggttca ggctcgactc gcgcacctgg tcgtaggtcg ggttgatgac
     9661 gtagcggcct tcgatcaggc cgacgcgcac gcccgcaatc ggcgtcgtga acgggatgtc
     9721 ggagaggtaa agcgcgagcg aagcgccggt gatggcgatc acgtccgggt cgttctcgtc
     9781 gtccgcagag atgacggagg cgatgacctg cgtctcgttg cggtagcctt cggcgaagag
     9841 cgggcggatg ggacgatcaa tcaggcggca ggtgaggact tccttttcgt tcggtcgtcc
     9901 ctcgcggcgg aagtagttgc cggggatacg accggcggcg tagttgtgct cgcggtactc
     9961 gaccgtgagc gggaagaaat cgatgccttc gcgcggcttg tcggcgccga ccgccgccac
    10021 gagcagcatc gtctcgccgt agccgatgac gacggagccg tcggcctgct tggcgatgcg
    10081 cccggtctcg accgtcagct ccttgtcgcc gagtttgatc ttctctttca gatacttctt
    10141 gactgccatg tctttgtctc cttcggacgc tccttctttc ggggaagggg cgcggcgtct
    10201 catccgccgc gcgcgcccgg cattaggtgt gttgtcggtg cctgccgcgc cgggccgcgc
    10261 tacgtgaggc gcgcccgctc gtcaggcgtc aaccaaaaaa aacggcacca cgcttcgtcc
    10321 gtgaagccgg tgccgccttt tgcttgggta cgctacaggc tgccgcccgc cgtccgccca
    10381 tcgagagctt gagcgccgtc acgttcggcc actcccgcag gcttgttaca ggacgcgggt
    10441 gtttggttaa gaggagtagt ccctcgttca acttaagcgt tccgaaccct tcggcacgca
    10501 cgcccacgcg gggcgcgccg ccatccctcg aaggctagcg gcgcaggccg agcttgccga
    10561 tgagttcgtg gtatccctcg atgtctcgcc gcttgaggta atcgagcagg ctgcggcgtt
    10621 gcgagaccat cttgagcagt ccgcgccgcg agtggttgtc cttcttgtgc gtcttgaagt
    10681 gctccgtcag ttcgttgatg cgctgcgtga gcagagcgac ctgcacctgc ggcgagccgg
    10741 tgtcgccttc gtgcgtccgg tactggccga tgatcgtttc cttggattcc gctgttagtg
    10801 ccactgttcg agatggtcct cccagaccgc agtcgcctgc ttgtatccac aggcaaccgc
    10861 aagagttagt aagtctagcc gaaacaatct agcacgcccc tccgcgcagt gtccagcgac
    10921 cagcagtcgc ggcgaagtgt gcgccgggcg gtttgaagga agtgcttgga cgcacgctcg
    10981 atgtccgttt cgcattgtgc gaaccctctc tccctctcgc cgcaaactgc ggcgccccgc
    11041 ccgccgcgtt aaagcccgat ccatccggaa cctttcggaa gccgttgggc gaggacgcgg
    11101 cacgggtctt gatgaaagca tgagcagcgc cgcgcatggt gcggggcgta aggggcgcgc
    11161 ggtgcggtgt gcgcccccgc gccgcctctg ctaccatgcc cgccggagta atcaacatcc
    11221 acgtttggag gcaatcgcat ggacgcctcg gaagagagag acccgcagcc cgcgcggatc
    11281 gtcgtcatcg gcggcggctt cggcggactg ttcacggcgc tcggcatggc gcgcgcgggc
    11341 gaagtcactt tgatcaccaa cgaagaccac ttcctgcaca cgccgatgct ctacgaatac
    11401 ctgagcggcg aggtcgaggc gtggcacatc gcgccgacct accgcgagct gctcggcgac
    11461 gaccgcgtgc gcttcgtgca gggcgccgtc gtgaacgtgg acttcgacgc gaaagaggtc
    11521 gaggtcgcgg ggcgcgtccg gcgtttggct tacgactacc tcgtgctcgc cgtcggcggc
    11581 gtcacgaact tctggggcgt cgagggcgcg gagaagtacg cgatgccgtt ccgcaagctc
    11641 cgccacgcgg acgacctgcg cgcgcggatg atcgagtcgc tcgaccgcgt ccagcccgac
    11701 gccgcgccgc aggacgcgcg ccgcgccgcc accttcgtcg tcgtcggcgc gggcgcctcg
    11761 ggcgtcgagc tggcgaccaa gatgtcggat ttgctgacgg acgccttccg tcgccgcggc
    11821 ttgcgcggcg agccgcgcgt cgtcgtcgtc gagatgggcg acgaggtcgt gcccggcatg
    11881 gccgacgaca tccgccgcgt ggtcgaagag gcgctgcggg agcggcgcat cgagctgcac
    11941 acgcgggcgc gcgtcgtgaa ggtgtcggag gacggcgcgg tgatcgagca cgagggcgag
    12001 cggacggaga tcgaagcgtc gggcgtcatc tggacggcgg gcgtcagggt gaacccgctg
    12061 gtcgagaagc tgaacgtcga gcgcgaccgg cgggggctga tcgtcgtcga gccttctttg
    12121 caagtgcgcg ggcgcgaggg cgtcttcgcg gtcggcgaca tcgcgctctt cacggacatc
    12181 ccgccgagcc tcgcgggcac ggcgcagttg gccttccagg agtcgtcgct cgtcgccgcc
    12241 aacgtgcagg cgctcatcga cggcggcgtg cccgcgcgcg gacacttcca ggaactcggc
    12301 gaggccgtca gcctcggcac agccgacgcc gccgtcctcg ccgccgggca ggtcgtcacc
    12361 ggccccctcg cgcggcaggc ccgcttcgcg ctctacacca cgcgcctgcc gacgtggcag
    12421 caccgcctca aggtcggcgc cgcgtggttc ttcggcggca agacgccgcg cccgctcgga
    12481 ctgtaaaaga agtggtgagg actgaggact gaggactgag ttaaggcaat ttgtcttaac
    12541 tcagtcctca gtccttttct tgcccgtcac tcgtcaaggc ttttagtcag ggtcgatgcg
    12601 ggcgggcggt tttacttcga agtgcgcgtg attggcgtga ccttgcgcgg ccatgcagag
    12661 accttcggcg acgagcacct gatcgctcaa gagtacgcgg ctcgccagcg gctgcctgcg
    12721 gaaggcgagg atcatggcgc gcatggcctt gcggtcgtag gcgttctggc cgacgacgcc
    12781 gccgcccgtc tgcccgttct tgcgcgggag gcggatgtcg caggccagcc ccgactcgtg
    12841 tgtggcgtgc atcggggtgt cgccgccctg cggcaggctg aagtcgttga gcgtgagcag
    12901 cgcggcgttc gtgttggcgc tgagatgctc ctctttgtac gaggcggcgg cggccttgag
    12961 cgtgtcggcg agccagctcg tgccgaagtc gtgcgcgtcg ttggtctgcg tcagctcgtg
    13021 gttgtggaac ccctcagcgg gcgagccggg cggcatctgc atccagcgcg gcgcgttctc
    13081 ggcgttgagc cacttgagcg tgtcgccgcc ggggtcgatc cgcccgtcgt tgccctgtat
    13141 gttgacctga ttgcgcccgt tcttgatcgc ctgaaagagc ttgatggcct tgacggtggt
    13201 cggccccatc acctcgtccg ccgtcagcca gtcgaacccg agttcgatga ggcgggtctt
    13261 gaccgcgagc acgtccgccg ccctgttgac ggcgttcagc ccgacactct tctgaatttt
    13321 aatcgctggc atgttctgtt tctcctccgc ttcttcgcgt tgaccgcagg ttgatgtccc
    13381 ctttacgact actaatgtcg tcgcggggtg aaacatttca gtggacgcgc ggaagcggcg
    13441 gcggacgccg cgagtggtt
//
LOCUS       DBNGOH010000926         2498 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2523758, whole genome shotgun sequence.
ACCESSION   DBNGOH010000926 DBNGOH010000000
VERSION     DBNGOH010000926.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2498)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2498)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2498
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2523758"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(129..1355)
                     /locus_tag="AC159P_18070"
     CDS             complement(129..1355)
                     /locus_tag="AC159P_18070"
                     /inference="COORDINATES: protein motif:HMM:NF025465.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6807 domain-containing protein"
                     /protein_id="HIR3697405.1"
                     /translation="MTRTLSILCAVACLALSLGHPRAGAARKDFYVTVAAGAHDRRES
                     VVAFALPAALNKKSYGLRDEAGQVIPLQVDAKRQAVFVLPELKAGATKTFRVVESKAV
                     GNSAARGVEVAQEGKRLVVKSGGRELLGFQTAPELPAGVKPVFARAGYIHPIYTPSGK
                     LVSDDYPSDHYHHHGIWAAWTKTEFEGRHPDFWNVADGTGRIDFVGVGQKWNGSVHGG
                     FTSRQRYVDVKATPEKAALEEEWEVTVYRVGEGPKAYAVFDLVSTQQTSGSSPLKLEE
                     YRYGGMGLRGHREWKDKSKVFFLTSEGKDRASGNATRGRWCHIGGPVGGHTVGIATFD
                     HPSNLRAPQPMRLNPDDPFFNWAPSQMGPFEIKPGASLVLRYRFVVADGPPDRAELDR
                     LWNDYATPPQVTITQK"
     gene            complement(1378..2460)
                     /locus_tag="AC159P_18075"
     CDS             complement(1378..2460)
                     /locus_tag="AC159P_18075"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HIR3697406.1"
                     /translation="MNHGLNIRQEGALDFLSLGALVHRLDPGVVPFRKATSCDIHVSG
                     GEFNCAANLADCFGLSTGIATAMVRYPIGDLIAERVKAMGVRPFYKFFEHDGVTGPNM
                     AAVYSDRGHGVRAPVVFYNRANEAAARLKPGDFNWGEIFAGGLRWFHTGGIFAALSET
                     TAEVIIEAMKAAKAAGAVVSYDLNFREKLWKIGGGYEHSLATVKRIVENVDVLVGNEE
                     DLQKGLGIRGQDVTAKSKLDPTAFFLMIEEVVERHPQVKVVATTLREVHTTNRHSWSA
                     VSWVNGETYSAPTAELDVIDRVGGGDGFASGLIYGMLSGESPEESLKLGWAHGALLTT
                     FPGDTTMATVEQVRSFAKGGSARIQR"
BASE COUNT          377 a          841 c          854 g          426 t
ORIGIN      
        1 cctgtcgtgc ttgcaaaacg ccgatcttgt gcagccagtc cagcccgtct ttccacgccg
       61 accgcgtaga cgtgtgcggc cccgtgcatg ttcgggcggc gcgcggctaa ctgctcaccg
      121 cttaccgctc acttctgcgt gatcgtgacc tgcgggggag tggcgtagtc gttccagagg
      181 cggtcgagtt cggcgcggtc gggcgggccg tcggcgacga cgaagcggta gcgcaggact
      241 agcgacgcgc cgggcttgat ctcgaacggc cccatctgcg agggcgccca gttgaagaag
      301 gggtcgtccg ggttcaggcg catcggctgc ggcgcgcgca ggttcgaggg gtggtcgaag
      361 gtggcgatgc cgaccgtgtg gccgccgacg gggccgccga tgtggcacca gcgcccgcgc
      421 gtggcgttgc cgctggcgcg gtctttgccc tcgctcgtca ggaagaagac cttgctcttg
      481 tccttccatt cgcggtggcc gcgcaggccc atgccgccgt agcggtactc ttcgagcttg
      541 agagggctgc tgccggaagt ctgctgcgtc gagacgaggt cgaagacggc gtacgccttc
      601 ggcccttcgc cgacgcggta gaccgtcacc tcccactctt cttcgagcgc ggccttctcg
      661 ggcgtcgcct tcacgtccac gtaacgctgg cgcgaggtga agccgccgtg cacagagccg
      721 ttccacttct ggccgacgcc gacgaagtcg atgcggcccg tgccgtcggc cacgttccag
      781 aagtccgggt ggcggccctc gaactcggtc ttcgtccacg cggcccagat gccgtggtgg
      841 tggtagtggt cggacgggta atcgtccgac accagcttgc ccgaaggcgt gtagatcggg
      901 tggatgtaac ccgcgcgcgc gaagacgggc ttgacgcccg cgggcagctc cggcgcggtc
      961 tgaaacccga gaagctcgcg gccaccggat ttaacgacga gccgcttgcc ctcttgcgcg
     1021 acctcgacgc cccgcgccgc cgagttgccg acggccttcg actccaccac gcggaaagtc
     1081 ttcgtcgcgc ccgccttcag ctccggcagc acgaacacgg cctgccgctt cgcgtccacc
     1141 tgcaaaggga tgacctgccc cgcctcgtcg cgcaagccgt aggatttctt gttcagcgcc
     1201 gcggggagcg cgaaggcgac gacgctctcg cgccggtcgt gcgcgccggc ggcgaccgtc
     1261 acgtaaaagt ctttgcgcgc cgcgcccgcc cgcggatggc cgagcgagag cgcgagacag
     1321 gcgacggcgc aaaggatgct gagagttctc gtcatttgga tttcaattgc ctatcgtcta
     1381 acgctggatg cgggccgagc cgcctttggc gaaggagcgc acctgctcga cggtggccat
     1441 cgtggtgtcg ccggggaacg tggtgagcag cgcgccgtgc gcccagccga gcttcaggga
     1501 ctcctcgggc gactcgcccg agagcatgcc gtagatgagg ccggaggcga agccgtcgcc
     1561 gccgccgacg cggtcgatca cgtcaagctc cgcggtcggg gccgagtagg tctcgccgtt
     1621 gacccacgag accgcgctcc aactgtgccg gttcgtcgtg tgcacctcgc gcagggtcgt
     1681 cgccacgacc ttgacctgcg ggtggcgctc gacgacctct tcgatcatca ggaagaaggc
     1741 cgtggggtcg agcttggact tggcggtcac gtcctgcccg cggatgccca agcccttctg
     1801 caagtcctcc tcgttgccga cgagcacgtc cacgttctcg acgatgcgct tgacggtggc
     1861 gagcgagtgc tcgtagccgc cgccgatctt ccacagcttc tcgcggaagt tgaggtcgta
     1921 cgagacgacc gcgcccgcgg ccttcgccgc cttcatcgcc tcgatgatga cctcggcggt
     1981 ggtctccgac agggccgcga agatgccgcc cgtgtggaac caccggaggc cgcccgcgaa
     2041 aatctcgccc cagttaaagt cgccgggctt cagacgcgcc gccgcctcgt tcgcgcggtt
     2101 gtagaagacg acgggcgcgc gcacgccgtg gccgcggtcg ctgtagacgg cggccatgtt
     2161 cggccccgtc acgccgtcgt gctcgaagaa cttgtagaag gggcgcacgc ccatcgcctt
     2221 cacgcgctcg gcgatgaggt cgccaatcgg atacctgacc atcgcggtgg cgatgcctgt
     2281 cgagaggccg aagcagtcgg cgaggttggc cgcgcagttg aactcgccgc cgctgacgtg
     2341 gatgtcgcac gaggtcgcct tgcggaacgg caccacgccg gggtcgagcc ggtgcaccag
     2401 cgcgccgagg ctcaaaaagt ccagcgcgcc ctcctgacgg atgttcagac cgtgattcat
     2461 tatcaatctc ctcaatcaca gtaggcagta gggggtag
//
LOCUS       DBNGOH010000927         4837 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3805311, whole genome shotgun sequence.
ACCESSION   DBNGOH010000927 DBNGOH010000000
VERSION     DBNGOH010000927.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4837)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4837)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4837
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3805311"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..187
                     /locus_tag="AC159P_18080"
     CDS             <1..187
                     /locus_tag="AC159P_18080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697407.1"
                     /translation="AFRLSTARSASALEVKIPATVFMLGKGFEREGGSRSGRLSPATL
                     PRPAQVRGFWSLELDAF"
     gene            277..651
                     /locus_tag="AC159P_18085"
     CDS             277..651
                     /locus_tag="AC159P_18085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S ferredoxin"
                     /protein_id="HIR3697408.1"
                     /translation="MIEVIEKHWPMSEGDGKATRKKRPRILAITTDCCTGCAGSPACI
                     EYCPIEACMFWVPDEDHPPFGRIEVDPYLCIGCQKCISKGPDGAFLDGCPWDAIEMVP
                     TDDWEAMHGITLPDAPPPAPAR"
     gene            complement(679..1557)
                     /locus_tag="AC159P_18090"
     CDS             complement(679..1557)
                     /locus_tag="AC159P_18090"
                     /inference="COORDINATES: protein motif:HMM:NF025030.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HIR3697409.1"
                     /translation="MPASNSNGHAAAEARGGPGDARRRTPWGLLVVAVLFVVVPFLAW
                     YGTWFGRALTDEQIEQYLGEADKPRHVQHALSEIEKRISKGDREAKRWYPRVVALAGS
                     QEKEVRLTAAWVMGADTRAAEFRERLAALTQDAEPIVRRNAALSLVGFADARALPDLR
                     AMLRPYSVRSVIGGEALTALKEGERVRRDSLLVRVDWANDHPPYEVRSPLDGVLEKVS
                     VKEGERVEAGRELFIIAPDPVAVGDALEGLRRLGTAEDLPEVERYAGGVERMPDYIKN
                     RAAQAAEAIKRRAGQG"
     gene            complement(1582..2007)
                     /locus_tag="AC159P_18095"
     CDS             complement(1582..2007)
                     /locus_tag="AC159P_18095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF420 domain-containing protein"
                     /protein_id="HIR3697410.1"
                     /translation="MNDSVLPHLNAALNATSGALLVAGFYFIMRRKVSAHLACMVAAL
                     VVSALFLVSYVVYHYNYGSVRFTGQGVVRTVYFVILITHVFLAAAIVPLVVLTVRRAW
                     RGDYARHRRVARWTLPLWLYVSVTGVIVYLMLYQLYPPR"
     gene            complement(2041..2286)
                     /locus_tag="AC159P_18100"
     CDS             complement(2041..2286)
                     /locus_tag="AC159P_18100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697411.1"
                     /translation="MAETEELTLRVLRMFDVMGKEALDLHELFEAGGNDPEARLAVFD
                     IVERLAREGALEERGNDFYALTEEGRARAARAGAGEE"
     gene            complement(2336..2569)
                     /locus_tag="AC159P_18105"
     CDS             complement(2336..2569)
                     /locus_tag="AC159P_18105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697412.1"
                     /translation="MTEELIPAVDVEKQTIRGRSCPKCGGKIIPSNRAVYQSTTTPDE
                     VFPLWECERCGYGEISEKKAPAKPAHAPKKGAA"
     gene            complement(2637..2873)
                     /locus_tag="AC159P_18110"
     CDS             complement(2637..2873)
                     /locus_tag="AC159P_18110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697413.1"
                     /translation="MPSRARTLTLLLAAALVAWPADAFAQCALCRTAAEQAGAETANA
                     LNLGILMLLIPPVAIFCTIFAVAVRHHKEGGGEE"
     gene            complement(2880..3500)
                     /locus_tag="AC159P_18115"
     CDS             complement(2880..3500)
                     /locus_tag="AC159P_18115"
                     /inference="COORDINATES: protein motif:HMM:NF015582.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HIR3697414.1"
                     /translation="MALESIKGLMKKSDLLLMGVCVVFAAVFLVLAATNATGAGNFFT
                     IDSLFFTTVCLLLALTFLAIPAMTLHERGVIRDAFGVPEGITAARRHEGEIHFEGSNK
                     LFFYVLGALLVLTLVEVVLAYIEFTNLIIMLTILMGLSIIKAALIIAYFMHLKFERMS
                     LVLSLVPALVICICLLFIFFPDGIRSRELRATRPAAAAATPPEGAH"
     gene            complement(3626..4312)
                     /locus_tag="AC159P_18120"
     CDS             complement(3626..4312)
                     /locus_tag="AC159P_18120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HIR3697415.1"
                     /translation="MSTEAHAGQVTAAEWGGGALPYRVGHRKLGMWLFIVSDAMTFSA
                     LLIGYSYVRVSSEQWPTPFKFSPSIVFSTVMTLCLLASSFTMVMAVKASAHNRLKSAR
                     RWIGATMVGGTAFLVLHSIEWANLIGEGLRPFGVAPGSHFAGMWAERWPLAPPLFGAS
                     FFSITGLHMFHVFTGVLYLGVIAARIGKMRHEDVEISGLYWHFVDLVWMFVFPLIYIL
                     SVDMGGGGAH"
     gene            complement(4319..>4837)
                     /locus_tag="AC159P_18125"
     CDS             complement(4319..>4837)
                     /locus_tag="AC159P_18125"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF012720.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HIR3697416.1"
                     /translation="SILMMFSALASALIVRMGLPGQSALPRTPVPSFLWVSTALIVLS
                     SATIVVALRALKRDDGGAFRLWLGATLVLGLGFLSAQLLAWRQLVAQGFYLASNPHSS
                     FFYVLTGLHGIHLVGGIAGLAYLLARALRTPAGGARGEVRLRTFADVVAIYWHFMDGL
                     WVFLFLLLFLWR"
BASE COUNT          838 a         1633 c         1620 g          746 t
ORIGIN      
        1 cgcattcagg ctctcgaccg cgcgctcggc gtcggccctc gaagtgaaaa taccggcgac
       61 agttttcatg ctgggtaaag gattcgagcg cgagggcggc tcacgctctg gccgcctttc
      121 accggccaca cttccacgtc ctgctcaggt tcgggggttt tggtcgcttg aactcgatgc
      181 attttaacac gagtcaagtc gttttcattg agggccgcca cgccgtcgtg ttaaagtgca
      241 gttgccattc gagagacacg cccaacggag cgaagcatga tcgaagttat cgagaagcac
      301 tggccgatgt cggaaggcga cggcaaggcg acgcgcaaga agaggccgcg catcctcgcc
      361 atcacgaccg actgctgcac cggctgcgcc ggctcgcccg cctgcatcga gtactgccct
      421 atcgaggcgt gcatgttctg ggtgccggac gaggatcacc cgccgttcgg gcgcatcgag
      481 gttgacccat acctctgtat cggctgtcag aagtgcatca gcaaaggccc cgacggcgcc
      541 ttcctcgacg gctgcccctg ggacgccatc gagatggtgc ccaccgacga ctgggaggcc
      601 atgcacggca tcacgctgcc ggacgcgccg ccgcccgcgc ccgcgcgctg acagcatttt
      661 cgagcccagc caaatttttc agccctgacc cgcgcgtcgc ttgatggctt ccgccgcctg
      721 cgccgcgcgg ttctttatgt agtcgggcat ccgctccacg ccgcccgcgt agcgctcgac
      781 ctccggcaag tcttccgccg tccccaacct cctcagccct tcgagcgcgt cgccgaccgc
      841 gaccgggtcg ggcgcgatga taaaaagctc gcggcctgcc tcgacccgtt ccccttcttt
      901 gacggacacc ttttcgagca caccgtccag cggcgaacgc acctcgtagg gcggatggtc
      961 gttggcccag tccacgcgca cgaggagcga gtcgcgcctg accctctcgc cctccttcag
     1021 cgcggtgagc gcctcccccc cgatcacgga gcggacgctg tacggcctga gcatcgcgcg
     1081 cagatccggc aacgcgcgcg cgtccgcgaa gccgacgagc gagagcgcgg cgttgcggcg
     1141 cactatcggc tcggcgtcct gcgtgagcgc ggccagacgc tcgcggaatt ccgccgcgcg
     1201 cgtgtcggct cccatcaccc acgcggcggt gaggcgcacc tccttctctt ggctgcccgc
     1261 gagcgccacg acgcgcggat accagcgctt cgcttcccgg tcgcctttcg agatgcgctt
     1321 ctcgatttcg gagagcgcgt gttgcacgtg gcgcggcttg tccgcctcgc cgaggtactg
     1381 ctcgatctgc tcgtccgtca gcgcgcgccc gaaccacgtg ccgtaccaag caaggaacgg
     1441 cacgacgacg aagagcaccg ccacgacgag gagaccccac ggcgtacgcc tgcgggcgtc
     1501 gcccggcccg ccgcgcgctt cggctgctgc gtgtccgttc gagttgcttg cgggcatggc
     1561 cgcttatccg tccgccccgg cttatcgcgg cgggtagagt tgataaagca tgaggtagac
     1621 gatgacgccc gtcaccgaga cgtagagcca gagcgggagc gtccagcgcg cgacgcggcg
     1681 gtggcgcgcg tagtcgccgc gccacgcgcg ccgcaccgtc aggacgacga gcgggacgat
     1741 ggccgccgcg aggaagacgt gcgtgatgag gatgacgaag tagaccgtcc gcacaacgcc
     1801 ctgccccgtg aagcgcaccg acccgtagtt gtaatggtag acgacgtagg agacgaggaa
     1861 gagcgccgag acgacgagcg cggcgaccat gcaggcgagg tgcgccgaga ccttgcgccg
     1921 catgatgaag tagaagcccg cgacgagcag ggcgccgctc gtcgcgttga gcgccgcgtt
     1981 caggtgcggc aggacggagt cgttcataag cggagtggcg cgcggggtgc gggcgccgcc
     2041 tcattcctct ccggcgcccg cgcgcgccgc tcgcgcgcgc ccctcttcgg tgagcgcgta
     2101 gaagtcgttg ccccgctctt cgagcgcgcc ctctctcgcc agccgctcga cgatgtcgaa
     2161 gacggcgagc cgcgcctccg ggtcgttgcc gcccgcctcg aacagctcgt gcaggtcgag
     2221 cgcctccttc cccatcacgt cgaacatccg caggacgcgg agcgtcagct cttcggtctc
     2281 ggccaacttc tcaacctctg tgcgggacgg acttcgccgt cgtcgtcgcg cccgctcaag
     2341 cggccccctt cttcggcgcg tgcgcgggtt tcgcgggcgc cttcttctcg ctgatctcgc
     2401 cgtagccgca gcgctcgcac tcccagagcg ggaagacctc gtccggcgtg gtcgtgctct
     2461 ggtagaccgc gcggttcgag gggatgatct tgccgccgca cttcgggcac gagcgaccgc
     2521 ggatggtctg cttctctacg tccaccgccg gtatcagttc ttctgtcatc gtgttcgttc
     2581 aggagagggg cgcgggcgcg tctcggcggc gccgtgtcgc gtcgatgccg ggcgcgctac
     2641 tcttcgccgc cgccctcctt gtggtgccgg acggcgacgg cgaagatcgt gcagaagatg
     2701 gcgaccggcg ggatgagcag catcaggatg ccgaggttca gcgcattggc tgtctcggcc
     2761 ccggcctgct ccgccgccgt gcgacacagc gcgcactgcg cgaacgcgtc cgccggccac
     2821 gcgacgagcg ccgcggcgag cagaagcgtc aaggtgcgcg cgcgtgaagg cataaggttt
     2881 cagtgcgccc cctcgggcgg ggtcgcggcg gcggcggcgg gcctggtcgc gcgcagctcg
     2941 cgcgagcgga tgccgtcggg gaagaagatg aagaggaggc agatgcagat gacgagcgcc
     3001 gggacgagcg agagcacgag gctcatgcgc tcgaacttga ggtgcatgaa gtaggcgatg
     3061 atgagcgccg ccttgatgat cgacagcccc atcaggatcg tcagcatgat gatgaggttg
     3121 gtgaactcga tgtaggcgag caccacctcg acgagcgtca gcacgagcag cgcgccgagc
     3181 acgtagaaga agagcttgtt gctgccctcg aagtgtatct cgccctcgtg gcggcgcgcc
     3241 gccgttatcc cctcgggcac gccgaaggcg tcgcggatga cgccccgctc gtggagcgtc
     3301 atggcgggga tggcgaggaa cgtcagcgcg agcagcaggc agacggtggt gaagaagagg
     3361 ctgtcgatgg tgaagaagtt ccccgcgccc gtggcgttgg tcgcggcgag cacgaggaac
     3421 accgcggcga acacgacgca gacgcccatc agcagcaagt ccgacttctt catcaacccc
     3481 ttgatcgatt cgagagccat ctcaggcggc ctccttcact tgttaacgat gaatgcggaa
     3541 tgatgaacga tgaatgagaa gcacgctgcc tgtcatcctt cagcctgtca tcgattcatc
     3601 attccgcatt catcattcat cactttcagt gcgccccgcc gccgcccatg tccaccgaga
     3661 ggatgtagat gagcgggaag acgaacatcc agaccagatc gacgaagtgc cagtagaggc
     3721 cgctgatctc cacgtcctcg tggcgcatct tgccgatgcg ggcggcgatg acgccgaggt
     3781 agagaacgcc ggtgaagacg tggaacatgt ggaggcccgt gatcgagaag aagctcgcgc
     3841 cgaagagcgg cggcgccagc ggccagcgct cggcccacat cccggcgaag tggctgcccg
     3901 gcgcgacgcc gaagggacgc agcccctcgc cgatgaggtt cgcccactcg atcgagtgga
     3961 ggacgaggaa ggccgtgccg ccgaccatcg tcgcgccgat ccagcgccgc gcgctcttga
     4021 gccggttgtg cgccgacgcc ttgaccgcca tcaccatcgt gaagctcgac gccagcagac
     4081 acagcgtcat cacggtcgag aagacgatgc tcggggagaa cttgaagggc gtcggccact
     4141 gctcgctcga cacgcgcacg tagctgtagc cgatcagcag cgccgagaag gtcatcgcgt
     4201 ccgagacgat gaagagccac atcccgagct tgcggtgccc gacgcggtag gggagcgcgc
     4261 cgccgcccca ctccgccgcc gttacctgtc ctgcgtgcgc ttcggtgctc aaacccggtc
     4321 acctccagag gaaaagtagt aggaagagga agacccagag gccgtccatg aagtgccagt
     4381 agatcgccac cacgtcggcg aaggtgcgca agcgaacctc gccgcgcgcg ccgcccgcgg
     4441 gggtgcgcag cgcgcgcgcc agcaggtacg ccagccccgc gatgccgccg acgaggtgga
     4501 tgccgtgcag ccccgtcagc acgtagaaga aagagctgtg cgggttcgac gcaagataga
     4561 acccctgcgc gacgagctgc cgccacgcga gcagttgcgc cgagagaaag ccgagtccga
     4621 gcacgagcgt cgcgccgagc cagaggcgga acgcgccgcc gtcatcgcgc ttgagcgcgc
     4681 gcagcgcgac gacgatggtg gcgctgctca ggacgataag cgccgtgctc acccacagga
     4741 acgaggggac gggcgtgcgc ggcagcgcgc tctggccggg cagccccatc cgcacgatga
     4801 gcgcgctcgc cagcgccgag aacatcatca ggatcga
//
LOCUS       DBNGOH010000928         3178 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_5089528, whole genome shotgun sequence.
ACCESSION   DBNGOH010000928 DBNGOH010000000
VERSION     DBNGOH010000928.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3178)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3178)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3178
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_5089528"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1272
                     /locus_tag="AC159P_18130"
     CDS             <1..1272
                     /locus_tag="AC159P_18130"
                     /inference="COORDINATES: protein motif:HMM:NF014385.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HIR3697417.1"
                     /translation="VAALLQEDGALERYVDATAGLTVEQAVQYALEHNGELLAVRKEV
                     DAARAMVRQASLRANPQVGAAGSKQLNGGDKSVMVEGVLPLELGGRRAARVTVAEREV
                     EMREQMLADRERMLAAEVRLKFGEVLAEILKLGFNDDQLNSSRRGYRLVSARVEEGRT
                     APLERSMTLVEVNRLLSMREAAEGRVQVAMLELKNVIGMTTEEPLRRRGDFIELLGTL
                     PALAEATEQALDVRPDLRAARAAESLAEAQIEQARAEGRPDASLRAGYQRMKQGFMVN
                     GIDDMGRLAPVEMTSHSVTFGVMIDLPVRNKNQGAIEAAVAQGEAAKRRREFLELTIR
                     REVASGYARYGKAARAMEIFRVGVRGQADANLEVIRKTYELGSKTLLDYIAEQRRFYE
                     VEAEFVDAVLNTYQARVEISRASGSPGLIKR"
     gene            1281..2693
                     /locus_tag="AC159P_18135"
     CDS             1281..2693
                     /locus_tag="AC159P_18135"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR3697418.1"
                     /translation="MEEERAKTVVETSAEDNEGGAEKSATNGARVETEARSEKAPRGD
                     TPRRRNKTAVVVVAVTALAALALVLWWFSRSGQGGAGRAVPAPRSVDMPEAATGGTVT
                     TAEPTVTLAPEVAARSGIKLEQVGEALAGGEGGAGQSATGVVQANAYKTTPVVSLVGG
                     IVRRVEAELGQHVRRGQAVAVVFSDELAMAQSKYLTALAELDEHHKHHHRTGQLVEIG
                     AASREELEMATTKLKSAESEVASLKQRLLLLGLTPQRIAGLRSPSQISSEVALPAPVS
                     GTVIERAANPGEVVETNKTILRVADLSSVWVIGQVYEKDLARVRVGSGASVTSDAYPG
                     RVFRGRVTYMDPQLDQATRTAQVRVELANPGQALKIGMYVNVAFGALGGAEATAPTVP
                     ASAVQNVGGRQVVFLATNDPSVFVMRPVRLGPESNGRRPVVEGLFVGDRVVTEGSFML
                     RAEWLKLNPGGGQAQQQHRH"
     gene            2741..>3178
                     /locus_tag="AC159P_18140"
     CDS             2741..>3178
                     /locus_tag="AC159P_18140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HIR3697419.1"
                     /translation="MNFPRPASGLLLLALIFIPRAVVAQQGTVRPSPASAERPYVYDD
                     GPVIVHTDLVTLNVTVTDAQGRHVAGLDKSAFTVLDNKATQEISFFDDADAPATVAVV
                     FDTSGSMSEGKIERAKEALARFIRTSHPRVEFYLIGFDSSAQVL"
BASE COUNT          567 a         1039 c         1121 g          451 t
ORIGIN      
        1 gtcgcggcgc tgctgcagga ggacggggcg ctcgaacgct acgtcgacgc taccgcgggc
       61 ctcacggtcg agcaggccgt gcagtacgcg ctcgagcaca acggcgagct gctggcggtg
      121 cgcaaggagg tagacgccgc acgcgcgatg gtcaggcagg cctccttgcg cgccaacccg
      181 caggtcggcg ccgccggctc gaagcagttg aacggcggcg acaagtcggt gatggtcgag
      241 ggcgtgctcc cgctcgaact cggcgggcgg agggcggcgc gcgtcacggt ggccgagcgc
      301 gaggtcgaga tgcgggagca gatgctggcc gaccgcgaga ggatgctggc ggcggaagtc
      361 cgcctgaagt tcggcgaggt gctcgcggag atcctcaagc tcggcttcaa cgacgaccag
      421 ctcaacagca gccggcgcgg ctaccgcctc gtctccgcgc gcgtggaaga gggacgcacc
      481 gcccccctcg aaaggagcat gacgctcgtc gaggtgaacc gcctgctctc gatgcgggag
      541 gccgccgaag ggcgcgtgca ggtcgcgatg ctggaactca agaacgtcat cggcatgacg
      601 accgaagagc cgttgcgccg gcgcggcgac tttatcgagt tgctcggtac acttcccgcc
      661 ctggccgagg ccaccgagca ggcgctcgac gttcggcccg acctcagggc cgcgagggcg
      721 gccgagtcac tcgccgaggc gcagatcgag caggcccgcg ccgagggccg ccccgacgcc
      781 tccttgcggg ccggctacca gcgcatgaag cagggcttca tggtcaacgg catagatgac
      841 atgggccgcc tcgcgcccgt cgagatgact tcgcactcgg tcaccttcgg ggtcatgatc
      901 gacctccccg tgcggaacaa gaaccagggg gcgatcgagg cggcggtcgc gcaaggagag
      961 gccgcgaaac ggaggcgcga gttcctggag ctgacgatca ggcgggaggt ggcctcgggt
     1021 tacgcgcgct acggcaaggc ggcgcgggcg atggaaattt tccgggtcgg cgtgcgcggt
     1081 caggcggacg ccaacctcga agtgatccgc aagacgtacg agctggggtc gaagacgctc
     1141 ctggactaca tcgccgagca gcggcgcttc tacgaggtcg aggcggagtt cgtggacgcc
     1201 gtgctgaaca cgtatcaggc gcgcgtcgaa atcagccgcg cgtcgggctc gccggggttg
     1261 ataaagagat gagtgtgagt atggaagaag agcgcgcaaa aacagtcgtc gagacctcgg
     1321 cggaagataa tgagggtggg gcagaaaagt ccgcgacgaa cggggcgcgc gtcgaaactg
     1381 aagcgcggtc agagaaggcg ccccgcggcg acaccccgcg gcgcagaaac aagaccgccg
     1441 tcgtcgtcgt cgccgtgacg gcactcgccg ccctggcgct cgtgctctgg tggttctcgc
     1501 gctcgggcca gggcggcgcc ggtcgcgccg tgccggcccc gcggtcggtc gacatgccgg
     1561 aggcggcgac cggcgggaca gtcacaaccg ccgagccgac cgtcacactc gcgccggagg
     1621 tggcggcgcg ctccggcatc aagctcgagc aggtcggcga ggcgctggcg gggggcgagg
     1681 gcggcgcggg gcagtcggcg acgggcgtcg tccaggcgaa cgcttacaag accacgccgg
     1741 tagtttcgct cgtcggcggc atcgtgcgcc gcgtcgaagc cgaattaggt cagcacgtcc
     1801 ggcgcgggca ggcggtcgcc gtcgtcttca gcgatgagct ggcgatggcc caatccaagt
     1861 acctgacggc gctcgccgag ctcgacgagc accacaagca ccaccaccgc accggccagt
     1921 tagtcgagat cggcgcggcc agccgcgaag agcttgagat ggcgacgacg aagctgaagt
     1981 ccgccgagtc cgaagtcgcc tcactgaaac agcgcctcct gctcctgggc ctgacgccgc
     2041 agcgcatcgc cgggctgcgc tcgccctctc aaatcagttc ggaagtcgcc ctgcccgcgc
     2101 cggtctcggg cacggttatc gagcgcgccg ccaaccccgg cgaggtcgtc gagacgaaca
     2161 agaccatcct gcgcgtcgca gacctctcat cagtctgggt catcgggcag gtttacgaga
     2221 aagacctcgc ccgcgtgcga gtcgggagcg gcgcgtccgt cacgagcgac gcctacccgg
     2281 ggcgcgtctt ccgcggtcgg gtgacttaca tggacccgca gctagatcaa gcgacgcgca
     2341 ccgcgcaggt gcgcgtcgaa ctggcgaacc ccggacaggc gctcaagatc ggcatgtacg
     2401 tcaacgtcgc cttcggcgcg ctcggaggtg ccgaagcgac agcgccgacc gtaccggcat
     2461 cggcggtgca aaacgtcggc ggccgtcagg tggttttcct cgctacgaac gacccgagcg
     2521 tgttcgtcat gcgtcccgtc cgtctcggcc ccgaatcgaa cggcagacgc ccggtggtcg
     2581 aagggctgtt cgtcggcgac cgcgtcgtca ccgagggaag tttcatgttg cgcgccgagt
     2641 ggctgaagtt aaaccccggc ggcggacagg ctcagcagca acatcggcat tgaagttaaa
     2701 gcaccgcttt ggtcatcatg tgaggaatga aaaggcgatg atgaattttc cgagacccgc
     2761 gagcgggcta ctcctcctcg cgctcatctt catcccccgc gccgttgtcg cccaacaggg
     2821 aacggtcagg ccgtcgcccg cgtcagcaga gcgtccgtac gtttacgacg acggccccgt
     2881 gatcgtccac accgacctgg tcacactgaa cgtgaccgtc acggacgcgc agggccggca
     2941 cgtggcggga ctcgacaaga gcgccttcac cgtcctcgac aacaaggcga cgcaggagat
     3001 ttccttcttc gacgacgcgg acgcgccggc gaccgtggcc gtcgtcttcg acacctccgg
     3061 ctcgatgagc gagggaaaaa ttgagcgggc gaaggaagcc ctcgcgcgct tcatccggac
     3121 cagtcacccg cgcgtcgagt tttacctgat cggcttcgac tcctcggcgc aagtcctg
//
LOCUS       DBNGOH010000929         2675 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3256507, whole genome shotgun sequence.
ACCESSION   DBNGOH010000929 DBNGOH010000000
VERSION     DBNGOH010000929.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2675)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2675)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2675
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3256507"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..541)
                     /locus_tag="AC159P_18145"
     CDS             complement(<1..541)
                     /locus_tag="AC159P_18145"
                     /inference="COORDINATES: protein motif:HMM:NF051148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF7779 domain-containing protein"
                     /protein_id="HIR3697420.1"
                     /translation="MTTSSQSDREAAAALAEAVDNLPLALEQAAAYVRQTGVSLADYL
                     KLFREQEEAGIPDTAQYPKPLATTCRISFQKLKEESPAAADLLTLCAFLAPDDIPLET
                     IAEGAENLPESFAATAGDMEALSRAAATLQDYSLAKVRAGSFLSLHRFVQSITRDHLT
                     QDAAKIWAEAAIHLVNEAFP"
     gene            complement(543..1571)
                     /locus_tag="AC159P_18150"
     CDS             complement(543..1571)
                     /locus_tag="AC159P_18150"
                     /inference="COORDINATES: protein motif:HMM:NF013124.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HIR3697421.1"
                     /translation="MAVRDLTMQQQLQKKHFFISYNKADISWAEWIAWELEAAGYSTI
                     VQVWDFRPGFNFVIQMQRASKEAERVIAVLSPNYLSAEFTQPEWATAFARDPLGEKGL
                     LLPVRVSECELEGLLPQIVYIDVVGLPEERAKDALLRGVKTERAKPVVKPPFPGAAQT
                     LMFPGLLPPVWNVPHQRNPDFTGREDFLDELWATLTSGQPGEAVQAVHGPGGVGKSQV
                     VLEYAYRHADKYRVVWWLRAEESSTLAADYASLYAKLELSPKDITDQSVMVNAVRERL
                     TQKDDWLLIFDNARGADDVSAYIPQPCAGDVLITSRNPGWQAFARERLLQPLAADDAV
                     NFLLKRTA"
     gene            2340..>2675
                     /locus_tag="AC159P_18155"
     CDS             2340..>2675
                     /locus_tag="AC159P_18155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010586556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA];
                     GO_process: GO:0006397 - mRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA recognition motif domain-containing protein"
                     /protein_id="HIR3697422.1"
                     /translation="MSTRLYVGNLNFRTTSDDLREHFSQVGEVESATVVEDRETGRSR
                     GFGFVEMATAEGAAAAIEQFNGKDFNGRNITVNEARPREDRGGRGGGGGYGGGGGRDR
                     GGYGGGGGGG"
BASE COUNT          447 a          816 c          948 g          464 t
ORIGIN      
        1 ccgggaaagc ctcattaacc agatggatgg cagcttcagc ccaaattttc gcggcgtcct
       61 gcgtcaaatg gtcgcgcgtg atcgactgca cgaagcggtg tagtgagagg aaactaccgg
      121 cgcgcacttt cgccagcgag taatcctgga gcgtggccgc cgcgcggctc agggcttcca
      181 tatcgcctgc cgtcgcggcg aaggactcgg gcagattctc cgcgccctct gcaatagttt
      241 cgagcgggat gtcgtcgggc gcgaggaacg cgcacagcgt gagcaggtcg gctgccgccg
      301 gagactcttc cttgagcttc tggaaagaga tgcggcatgt ggtggctagg ggcttcgggt
      361 attgcgccgt gtcgggtatg ccggcctctt cctgctcgcg gaacagctta aggtagtcgg
      421 cgagcgaaac gccggtctga cggacgtagg ccgcggcctg ctccaacgcg aggggcaggt
      481 tatcgacggc ctcggccaga gccgcggcgg cttccctgtc gctctgtgaa ctggtcgtca
      541 tctcaggccg tcctcttcaa aaggaagttc accgcgtcgt ccgcggcgag cggttggagc
      601 aggcgttcgc gcgcgaacgc ctgccagccc gggttgcgcg acgtgatgag cacgtcgccc
      661 gcgcagggct gcgggatgta tgcgctcacg tcgtcggccc cgcgcgcgtt gtcgaagatg
      721 agcagccagt catccttctg cgtcagccgc tcgcgcacgg cgttgaccat gacgctttgg
      781 tcggtgatgt ccttgggcga gagttccagc ttcgcgtaga ggctcgcgta gtcagccgcg
      841 agtgtcgaag actcctccgc gcggagccac cacacgacgc ggtacttgtc tgcgtggcgg
      901 taggcgtact ccaacactac ctgcgacttg ccgacgccgc caggcccgtg gacggcctgc
      961 accgcctcgc cgggttggcc cgaagtgagc gtggcccaca gttcgtcgag gaagtcttcg
     1021 cggcccgtga agtcggggtt acgctggtgt gggacgttcc agaccggggg cagcaggccg
     1081 gggaacatca acgtctgtgc ggcgcccgga aaaggcggct tcaccaccgg cttggcgcgc
     1141 tcggtcttca ccccccggag gagcgcgtcc ttcgccctct cctcgggcag ccccacgaca
     1201 tcgatgtaga cgatctgcgg gagcagccct tcgagttcgc actcagacac gcgcacgggc
     1261 agcagcagcc ccttctcgcc gagcgggtca cgagcgaagg ccgttgccca ctcgggttgc
     1321 gtgaactcgg cgctcaggta gtttggtgag aggacagcga tgacgcgctc ggcctccttg
     1381 gacgcgcgct gcatctggat gacgaagttg aagccgggcc tgaagtccca cacctgcacg
     1441 atggtcgagt agcccgccgc ctccaattcc cacgcgatcc actcggccca cgagatgtcg
     1501 gccttgttgt agctgatgaa gaagtgtttc ttttggagtt gctgctgcat ggtgaggtcg
     1561 cgaacggcca tccacgcgat aagggatttc gaaaagctgt cgccgcctga agcgggccgg
     1621 gcgcgtgccc gaccgcgcca ttgagcaata ccttatgcgg cgccggtttt caagaaaaag
     1681 ttccgggcgg ggcgggcgcg cgtggcgtat aatcggcggc ggatggggga gtagatcgcc
     1741 cacagagggt tcggcgaagt cgtcagcacg gtcgcccgcc tgaacaggga agccgaaggc
     1801 ggcccggctc gccggtgcgt aagatcgaga cctttcgcgg ctatgatggc gtgcgggagg
     1861 tctttctctt tcggcgcgcg aggcgcaaga gaggagggcg cgggcgatgg cggaattcgt
     1921 gttggagtgt ctgtggttgg cgtgcgagcc ggcgctcgaa gtcggcgccg atgttctcga
     1981 actgtggggg gcgtccggcg ggcgcgagcg cgactgagcg cggagggcgc ggcggaaaaa
     2041 tcggcttgcg ctcgggcgga aatcccggca tacttcagcg cgtcaggttc ctgctctcgc
     2101 gcgtcagtgt tgcacgcgcc tgcccgtgac agtttcggcg acggtgttcg gctgcaagga
     2161 gagtgaggtt ggtgattgac aggtcagaag ccgctctcgc tagacgttag agcatcgggc
     2221 aacttggtcg ccgccgcttt cgccggaacg gtcgttggaa agactccccg tctcacgcac
     2281 cccgcgccgc aaacggcaag cccgcgcttc ttcctcttca gataaaggac agacaaaaga
     2341 tgagcacgag actttacgtc ggaaacctca atttccgcac caccagtgac gacctgcgcg
     2401 agcacttctc acaggtgggc gaggtcgagt ccgcgaccgt ggtcgaagac cgcgagacgg
     2461 ggcgctcgcg cggcttcggc ttcgtcgaga tggcgacggc cgagggcgcg gccgcggcca
     2521 tcgagcagtt caacggcaag gacttcaacg ggcgcaacat caccgtcaac gaagcccgcc
     2581 cgcgcgaaga ccgcggcggt cgcggcgggg gcggcggcta cgggggagga ggcgggcgtg
     2641 accgcggcgg ctacggcggc ggcggcggcg gcggg
//
LOCUS       DBNGOH010000930        10602 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4722649, whole genome shotgun sequence.
ACCESSION   DBNGOH010000930 DBNGOH010000000
VERSION     DBNGOH010000930.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 10602)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10602)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10602
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4722649"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(11..1480)
                     /locus_tag="AC159P_18160"
     CDS             complement(11..1480)
                     /locus_tag="AC159P_18160"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019532912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HIR3697423.1"
                     /translation="MTPLAGETRAGVAAPFSYARQKAGRFVAELMDFVRFPSVSAQAR
                     HDEDVRACAAWLAGHLREIGMENVSLTPTPRHPIVQADWLHAAGRPTVLLYGHYDVQP
                     PDPLEEWRTPPFEPVVRGGDLYGRGASDDKGQMFCHVKALESLLRTAGALPVNVRCLF
                     EGEEEVGSSGLQEFLRHRRAALAADLAVMSDMRMRAPGRPAITYALRGALSLELEVSG
                     PEHDLHSGNFGGAIHNPLQALCEIIAGLHDARGRVAVPGFYDTVRGASEREREYMARV
                     GPSDEDLLRDARCRAGWGEEGYSFYERVGARPALTVNGITGGYQGEGSKAVIPSRAAA
                     KLNIRLVPDQDPSDIERLFRQHVRRITPPTVRAAVRTTLAARPALIERNHPALRAAAW
                     AYRRGFGAAPVFLRSGGTIPVVNSLQELLGLPTVLMGFAWPDDRMHAPNEKFHLLNFF
                     KGIETSIHFLSAVARAPARGGHAATDAGRFGRPERVLTA"
     gene            complement(1533..2108)
                     /locus_tag="AC159P_18165"
     CDS             complement(1533..2108)
                     /locus_tag="AC159P_18165"
                     /inference="COORDINATES: protein motif:HMM:NF013625.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HIR3697424.1"
                     /translation="MSREFNHEIDAVRALAGYGGAFEPLGFEAAAGWQGEVGRGSPAY
                     VSWVQHSLNQVLGRRLAVDGQMGPQTRSAIRSFQQKKGLTTDGVVGAQTEAALVAAGA
                     PPPGTSTPAPAQTGVIDPRIDVHAQRSLQRLLKTERAGVADLARSIHGAVKGEGGQLH
                     ADGLAFASERREPCTPAVGDQGRGRRHLLTP"
     gene            complement(2141..3259)
                     /locus_tag="AC159P_18170"
     CDS             complement(2141..3259)
                     /locus_tag="AC159P_18170"
                     /inference="COORDINATES: protein motif:HMM:NF013625.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HIR3697425.1"
                     /translation="MRYGRYVYAEPFAGYTEFDELDMFEAETGAVNRTSPAYVRWVQG
                     SLNRIMGLRLAVDGQIGTQTRSAVRSFQQSRGLQADGDVGPQTERALVAAGAGPPPAA
                     GAAPAPPGPAPRSTPGLMKSETAPPSSTLYVDIALRSEGQARPMTGIFIPQDYRPQPP
                     VDLILYLHGFKYTQAWKPYPTLSIDGYWDARQFPYFTLRERLNESRKNVILVAPTLGA
                     RSEAGLLTRPGGFDAYLDQVMAALAAHGPYRNAGQPPRPGNIILACHSGGGLPMRQLA
                     LSNQRYSPQIKECWGFDSTYNRGDDTEWARWARSRSGVGVYIYYVANSRTEPLAVGLK
                     RQKAPNVFVVPSPEHRHNWVPITHWRERIAAASFLRSV"
     gene            complement(3330..6140)
                     /locus_tag="AC159P_18175"
     CDS             complement(3330..6140)
                     /locus_tag="AC159P_18175"
                     /inference="COORDINATES: protein motif:HMM:NF018597.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HIR3697426.1"
                     /translation="MSYPFDYSAEPFQGFTKFDEFASSDEFDEFGVFGEGEFDEFDEF
                     GEEEFGEPEVFGYGFETEEERGGMHRVPGRASASSGRPPRGRPPAGRPLRARPPRAGR
                     PLPSGRRPGARPGRPGTKPPGRPPGRPKPPRPRPRYPWPIYKPWPGVFPVEAYPQVGR
                     FGNWFRSGDQLILLLDEDATPLAGGAEGAGAPAPAPNGGAPGEPNGGAPAGADAGAPS
                     EPDAGAQAEPPEGAEGELYGAGRRPSWRVRRLLRRVAPRTRWAATNQEFNFEMDPLRA
                     LAEYGGAFGQFGGGGERETTAAGKPPSRAAAATPVGPFGTLFANAPGRKPFQYGFTAE
                     DVLWTARFLVGEAGGRDNLDNQAVIWAMLNRYALFTHRYYPTFHRFIRAYSTPLQPVL
                     KSSGAAKRHMHKSEFVRTGGTYEGTDIPRGQLARFLKLQQTPWGKLPASARSLAERAL
                     KGHVANPIGNASEFASTRVYFRDRYKRWPQDFEEWRRFTESFAAGKKWVWVGPIAGLD
                     PMNNTFFVQKSVANLAPGTVRVVAGSPSVTQGGGTNQEAGYGDQQFYYAGAPYELESG
                     ADWQGEVNRRSRDYIRWVQSSLNRLLSLQLAVDGQIGTQTRSAVRSFQQRAGLPADGD
                     VGPQTEAALVRAGAPSPPGAGASTQTPSGIGGASGGQASGAQPPAPTGPSSGLPGVVS
                     TPCGVRTTKPATDRCTGPVLPTCPPVPDLLCVTSVSGIPFEYVMNSGWVVKGAGGLYV
                     VKNRSARKQNFTPAVASAVARFITNMASIGMPVEAILTGGSYYCRCISHTNTLSNHSF
                     GDAIDVAGIRWRSPTGSRETIVHNYANPGERAILRRINACLRLSFANVIDYHRSDHRD
                     HFHCDTNRGKGRYPKGSTSVVFIKEALNTIMGLGIPETTAYDAATAKGLRDFSGRTAD
                     ELSNTATLNKVLDDLFARVARG"
     gene            complement(6166..6981)
                     /locus_tag="AC159P_18180"
     CDS             complement(6166..6981)
                     /locus_tag="AC159P_18180"
                     /inference="COORDINATES: protein motif:HMM:NF015376.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HIR3697427.1"
                     /translation="MRPFYNMGVGSAQGLTEFDELALDPELEALLFPEGEDESEGEIR
                     TDSAYIRWVQSSLNQIMGLRLAVDGIKGVQTTSAVRSFQQQRGLTADGVVGPQTEAAL
                     VFAGAGAPPTSTSPSPTPGTGTCTIPNVTAQLPPTGPGFYGTTSSSRRFGTQQTIQAL
                     VTVGARWAAAHPTGPRIGIGDISLRCGGSMPPHVSHTKGVDADIRIMRRDGREEGTVY
                     QSSNYSRALTQELVNLFHANGVARVQYIFFNDPAVTGVRPWPGHDNHLHVRFY"
     gene            complement(6995..8086)
                     /locus_tag="AC159P_18185"
     CDS             complement(6995..8086)
                     /locus_tag="AC159P_18185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012647308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR3697428.1"
                     /translation="MRGVSPSLDFASALYLGLRHPSRCVPAWEQLTTGAPAALTRPPG
                     AAEVSRRLALLQGCESATLAPSTLHLFWDFFGTFGGRPATVYVDEVTYPIALWGVERA
                     RALGARVYAFAHHDPDDLRRLLLRSARAGSAQLVVTDGFCPDCGRLAPLAEYLAAARS
                     FGGRLILDDTQALGIFGEGPGRASPYGGGGGGGLRWHGLRDPLVLSISSLAKAFGVPL
                     AVLAGGAEAVHGFEELSETRVHCSPPSVAVLHAARRALEINLESGDELRRRLALRVRH
                     FRRRLREIGVTCRGGPFPVQTLQVGDSAARRIHAALLRASVKTILRRAHTREGAHLTF
                     SITARHRRRDIDYATGLLARCLEPETRSA"
     gene            complement(8083..9972)
                     /locus_tag="AC159P_18190"
     CDS             complement(8083..9972)
                     /locus_tag="AC159P_18190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012647307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697429.1"
                     /translation="MSKKLAEAKAANGEYHYEVTLKALPDRITGGGVVKLVAEPGDDD
                     INAHSFRFWIQPDPASATEVELKKLTGEPNAAVWTVTDNTPRGVRARVEMFTADGEGP
                     VQARDDADITVTQELLTADDLPPVLQGPTVPVSGSISLNRAASQVTGDQVLWSVIRDR
                     TTAIGFLNYKQFIDNLLCANPLQGRAGFDLRLPFPGVAAYAVLKAATEMFLMQECGVV
                     FENTTDPLLISRQETEESARLGRPVTRTELEGLRRAYLADLKAENAQALPYFDLIRQR
                     LAELAIKPADAVPGVDCYGILRSRFTAPCMIELIWSYWHEEGMLAQTMNAISYRFQNK
                     RIVPGNRDPLAHFDIDPLRGLSNFLWGYVQDEQHRLGVLRRAYEYDHQYGLRLYGKAV
                     QNFDPVDSRSKFLEAFHNLLYRAALFYERDDDTTIIADGFEVLNALREVHLILSYGAH
                     NQFGDLPSNARQEMLIEQWLLARPEMREFLGGKPMVAYTEPWMDRVDTVKSMFGWMDS
                     SVTYFHDLAVNGEQIVLSVRYGNWTQVNDMAAATNWARYWRPEIQRYIHAYRAVTGVD
                     LGVELVDSGRADERYVQPSIHLRNRLAQQRGGGALPPGDRRALGPGVSAGQAGESLAV
                     RNRNR"
     gene            complement(9986..>10602)
                     /locus_tag="AC159P_18195"
     CDS             complement(9986..>10602)
                     /locus_tag="AC159P_18195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013219402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697430.1"
                     /translation="SVARIGVGATVGLMGVVSLPSAFVFRVNLDDPHPFPWIRVKLSC
                     AIGKALYPHRQWERMAQLWESFYPLEELDATTRSLLVALEAGIPSFVTLLVNHRPRLL
                     RGASLREAMAVHEREPARLSAYFSAWRRSPRLMRETPPSLAFAVLGQARAEGRLSPER
                     ESRMLAYLLTYWALRSTLNASAGHAQPYRCGCQSQATDRGPSLQ"
BASE COUNT         1652 a         3576 c         3616 g         1758 t
ORIGIN      
        1 acctccgact tcaggcggtc agaactcgct ccggtctccc gaaccgtccc gcgtccgtcg
       61 cggcgtggcc gcctcgggct ggcgcccgcg cgacggcgga aaggaagtgg atggaggtct
      121 cgatgccttt gaaaaagttg agcagatgaa acttctcgtt gggcgcgtgc attcggtcgt
      181 cgggccacgc gaagcccatc aggacggtcg gcaggccgag caactcctgg agggagttga
      241 cgaccgggat cgtcccgccg gagcggagaa aaacgggcgc cgccccgaag ccgcggcggt
      301 aagcccacgc cgccgcccgg agggccgggt ggtttcgttc gatgagtgcg ggccgtgccg
      361 cgagcgtcgt gcgcaccgcc gcgcggacgg tcggcggggt gatgcgcctg acgtgttggc
      421 ggaaaaggcg ttcgatgtct gaagggtctt gatcgggcac gaggcggatg ttgagcttcg
      481 ccgcggcccg cgaagggatg acggccttgc tgccttcacc ctgatagccg ccggtgatgc
      541 cgttgaccgt cagggccggg cgcgcgccga cgcgttcgta gaacgagtaa ccctcctcgc
      601 cccaccccgc acggcaccgc gcgtcacgca gcaggtcttc gtcggaaggg ccgacccgag
      661 ccatgtactc ccgctcgcgc tcgctcgccc cccgcacggt atcgtaaaag ccggggacgg
      721 ccacacgccc gcgggcgtcg tgcagcccgg cgatgatttc gcagagcgcc tgaagggggt
      781 tgtgaatcgc gccgccgaag ttgcccgagt gcaggtcgtg ctccggcccg ctgacttcca
      841 gttcaaggct gagcgccccg cgcaacgcgt atgtgatggc ggggcggccc ggcgcgcgca
      901 tccgcatgtc cgacatcacg gcgaggtcgg cggcgagcgc ggcgcggcgg tggcgcaaaa
      961 actcctggag gcccgaactg ccgacctcct cctcgccctc gaaaagacac ctgacgttga
     1021 cgggcagcgc gcccgccgtg cgtagcaaag actccagcgc cttgacgtgg cagaacatct
     1081 gccccttgtc gtcgctcgcg cctctgccgt agagatcgcc gccgcgcacc accggctcga
     1141 acggcggcgt ccgccactct tcgagcgggt cgggcggctg cacgtcgtag tgcccgtaga
     1201 gcagtacggt ggggcggccc gcggcgtgca gccagtccgc ttggacaatc gggtggcgcg
     1261 gagtaggggt gagactgacg ttctccatgc cgatctctcg caagtgcccg gcgagccaag
     1321 ccgcgcaggc gcgcacgtct tcgtcatggc gggcctgcgc gctgacgctc gggaagcgga
     1381 cgaagtccat cagctcggcg acgaaacgcc ccgccttctg ccgcgcgtac gagaaggggg
     1441 cggcgactcc ggcgcgtgtc tcacccgcga gtggcgtcat ggatgcctcc ggcctgtaag
     1501 ccgcgccgcg ccgtggcttc gcggccctct cctcacggcg ttaacaggtg acgccggccg
     1561 cgcccctgat ctcccaccgc gggagtgcaa ggctcacgac gttcagaggc gaaggcaagc
     1621 ccatcggcat ggagctgacc accctcaccc ttgacggccc cgtggatgct ccgcgccaga
     1681 tcggcgacgc cggcgcgctc ggttttgagt agcctttgaa gcgagcgctg tgcgtgcacg
     1741 tcgatgcgcg ggtcgatgac gccggtctgg gctggggccg gcgtcgaggt gcccggcggc
     1801 ggcgcccccg ccgcgacgag tgcggcctca gtctgcgcgc cgaccacgcc gtcggtcgtc
     1861 aggcccttct tttgttgaaa gctgcggata gcgctgcgcg tctgcggccc catctggccg
     1921 tctaccgcca gccgccgccc caagacctga ttgagggagt gctgcaccca actgacgtac
     1981 gcggggctgc cgcggcccac ctcgccctgc cagcccgccg ccgcctcgaa cccgagtggc
     2041 tcaaacgccc cgccgtaccc ggccaaggcg cggacagcgt cgatctcatg attgaattca
     2101 cgactcatcg cgggtactcc cctcggacgc aaccgttgcc tcaaacgctc cgtaagaagg
     2161 aggccgcagc gatgcgctca cgccagtgcg tgatcggcac ccagttgtgc ctgtgctcgg
     2221 gcgatggcac gacgaacacg ttcggggctt tttgccgctt caggccgacc gccagtggtt
     2281 cggtgcggct gttggcgacg taatagatgt agaccccgac cccggagcgc gacctggccc
     2341 agcgcgccca ctcggtatcg tcgccccggt tataggtgga gtcgaacccc cagcactcct
     2401 ttatctgtgg cgagtagcgc tggttcgaga gcgccaactg ccgcatgggc aagccgccgc
     2461 cggaatggca ggcgaggatg atgttgcccg gtctgggggg ttgtccggcg ttcctgtaag
     2521 gcccgtgggc ggccagcgcg gccatcacct gatcaagata cgcgtcgaac ccgccgggcc
     2581 tcgtcaacag ccccgcctcc gaacgcgcgc cgagcgtcgg cgcgacgaga atcacgttct
     2641 tccggctctc gttcagcctc tcgcgcaggg tgaagtaggg gaactggcgt gcgtcccagt
     2701 acccgtcaat cgacagcgtg gggtagggtt tccacgcctg cgtgtatttg aacccgtgca
     2761 ggtaaaggat gagatcaacc ggcggctgcg gtcggtagtc ctgcgggatg aagatgccgg
     2821 tcatcgggcg cgcctgacct tcactacgca gggcgatgtc aacgtagagg gtggaggacg
     2881 gcggcgccgt ctcgctcttc atcaagccgg gggtcgaacg cggcgcgggg ccgggcggcg
     2941 cgggcgcggc gccggcggcg ggaggcgggc cggcgccggc cgcgaccaac gcccgttcgg
     3001 tttgtggccc cacgtcgcca tcggcctgca agccacggct ctgctggaag ctgcggacgg
     3061 cgctgcgcgt ctgcgtcccg atctgcccgt ccaccgccaa ccgcagcccc atgatccgat
     3121 tgagcgagcc ctgtacccag cggacgtacg cgggactcgt gcgattaact gcgcccgtct
     3181 ccgcctcgaa catgtccagc tcgtcaaact ccgtgtagcc ggcgaacggt tcggcataaa
     3241 cataccttcc gtatctcata actaacctcc cggtgagtta atcaacttcg gtgaagagag
     3301 gcttacgggc gaccgttgct tctgtccgct tatccgcgcg ccacgcgtgc gaagaggtcg
     3361 tccaggacct tgttcaaggt ggccgtgttt gacaactcat ctgcggtgcg tcccgaaaaa
     3421 tccctcaagc ccttcgccgt ggccgcgtcg taggccgtgg tctccggtat ccccagaccc
     3481 atgatcgtgt tcaacgcctc tttgatgaac accaccgacg tgctgccttt cggatacctg
     3541 cctttgccgc gattggtgtc gcagtggaaa tggtctcgat gatccgaacg gtggtaatcg
     3601 atcacgttgg cgaaagacag gcgcagacag gcgttgatgc ggcggaggat cgcacgctcg
     3661 cccgggttgg cgtagttatg gacgattgtc tcgcggctgc ccgtcggact ccgccagcgt
     3721 atgccggcga catcaatcgc gtcgccgaaa gagtggttac tcagggtgtt cgtgtgcgat
     3781 atgcaacggc agtaataaga accgccggtg agaatcgctt cgacaggcat cccgatgctc
     3841 gccatgtttg tgatgaagcg ggccacggcg ctggctacag ccggggtgaa attttgctta
     3901 cgggcgctcc gattcttgac gacatacaag ccgcccgcac ccttcaccac ccagccgcta
     3961 ttcatcacgt attcgaaggg tatcccgctc acgctggtga cgcacaggag atcgggtacg
     4021 ggcgggcagg tcggcagaac cggccccgtg cagcgatccg tggccggctt ggtggtccgg
     4081 accccacacg gcgtgctgac cacgcctggc aggccggacg aagggccggt cggggcgggc
     4141 ggttgcgcgc cggacgcttg tccgcctgaa gccccgccaa tgcctgaggg tgtctgggtg
     4201 gacgcgccag cgcccggcgg ggaaggcgca ccggctctga ccagtgcggc ttcggtctgt
     4261 ggccccacgt cgccatcagc cggcagcccc gcgcgttgct ggaagctgcg gacggcgctg
     4321 cgcgtctgcg tcccgatctg cccgtctacc gccagctgca agctcagcaa ccggttgagc
     4381 gagctttgta cccagcggat gtagtcgcgg ctacgacggt ttacctcgcc ttgccagtcc
     4441 gcgccggact ctaactcata cggcgcgccg gcataataga attgttggtc gccgtagcct
     4501 gcctcctgat tagtgccgcc gccctgtgtc acgctcggcg agccggccac gacccgcacc
     4561 gtgccgggtg cgagattcgc cacgctcttc tggacgaaga aggtattatt catcgggtct
     4621 aaccccgcga tggggccgac ccagacccac tttttgccgg cggcgaaaga ctcggtgaag
     4681 cgccgccact cttcgaaatc ctgcggccac ctcttgtacc ggtctcgaaa gtaaacccgc
     4741 gtcgaagcga actcactggc gttgccgatg gggttggcga cgtgcccctt caacgcgcgc
     4801 tcggccagcg accgggcgct cgcgggcaac ttgccccacg gcgtctgttg tagcttcagg
     4861 aagcgcgcca gttggccgcg ggggatatcc gtcccctcgt acgtcccgcc ggtgcgtacg
     4921 aattccgatt tgtgcatgtg ccgcttggcg gcgccggaac ttttgagcac gggctgtaaa
     4981 ggcgtcgagt aggcgcggat gaaccgatga aaggtcgggt agtagcggtg cgtgaagagg
     5041 gcgtagcggt tgagcatcgc ccagatcacc gcctggttgt cgaggttgtc gcgcccgccg
     5101 gcttcgccga ccaggaaacg ggccgtccac aagacatcct ccgcggtgaa cccgtattgg
     5161 aacggcttgc gaccgggggc gttggcgaac agcgtgccga aagggccgac cggtgttgcc
     5221 gccgctgctc gtgacggtgg tttacccgcc gcggtagtct ccctttcgcc tccgccgccg
     5281 aactgcccga aggccccgcc gtactcggct aaggcgcgca aggggtccat ctcgaagttg
     5341 aattcttgat tcgtcgcggc ccatcgcgtt cgtggggcaa cccgccttag caggcggcgt
     5401 accctccagg agggcctgcg cccggcgccg tacagttcgc cctccgcacc ttcgggcggc
     5461 tcggcctgcg cccctgcgtc cggttcactc ggcgcgcctg cgtccgcccc ggcgggggcg
     5521 ccgccgttcg gctcgcctgg ggcgccgccg ttcggggcgg gggccggggc gccggcgccc
     5581 tcggctccgc ctgcaagggg cgtggcgtct tcgtcgagca gaaggataag ttggtcgccg
     5641 ctgcggaacc agttcccgaa gcgtccgacc tgggggtacg cctcgacggg aaagacgccg
     5701 ggccacggtt tatatatagg ccacggatac ctgggccggg gacgcggggg cttgggccga
     5761 cccgggggtc ggcccggcgg cttcgttccg ggccgacccg ggcgcgctcc cggtcgtctg
     5821 ccggagggca gcggtcggcc ggcgcgcggg gggcgtgcgc gtaaagggcg gcccgccggt
     5881 ggacggccac gcggcgggcg accggacgac gcgctcgcgc ggcccgggac gcgatgcatt
     5941 ccgccgcgtt cctcttccgt ctcgaagccg tagccgaaca cctccggctc gccgaactct
     6001 tcttcgccga actcgtcgaa ctcgtcgaac tccccctcgc cgaacacgcc gaactcgtcg
     6061 aactcgtccg acgaggcgaa ctcgtcgaac ttcgtgaacc cctggaaagg ctccgcgctg
     6121 taatcgaatg ggtagctcat gaccgatctc ccttaaacca cacgcctagt agaaacggac
     6181 atgcaggtgg ttgtcgtggc ccggccacgg cctgacgccc gtcaccgcag ggtcgttgaa
     6241 gaagatgtac tgcacgcgcg ccacgccgtt ggcgtggaac aggttgacga gttcctgcgt
     6301 gagcgcccgc gagtagttgg aggactggta gaccgtgccc tcttccctcc catcccggcg
     6361 catgatgcgg atgtcggcgt caacgccctt cgtgtgcgag acgtggggcg gcatcgaccc
     6421 gccgcacctg agactgatgt cgccgatgcc gatgcgcggg ccggtcgggt gggccgcggc
     6481 ccagcgcgcg ccgacggtca cgagggcctg gatggtctgc tgcgttccga agcggcgtga
     6541 gctgctggtg gtgccgtaaa agcccggccc ggtgggtggg agctgggcgg tgacgttcgg
     6601 gatggtgcaa gtgcccgtgc cgggcgtcgg cgagggggac gtactcgtcg gcggggcgcc
     6661 ggccccggcg aagacgagcg cggcctcggt ctgcggcccg acgacgccgt cggcggtcag
     6721 gccgcgctgc tgctggaagc tgcggacggc gctggtggtc tgcaccccct tgatcccgtc
     6781 caccgccaac cgcagcccca tgatctgatt gagcgaactc tgcacccagc ggatgtaagc
     6841 gctgtcggtg cggatttcgc cttccgactc gtcttcgcct tccgggaaga gtagggcttc
     6901 gagttcgggg tcgagcgcca actcgtcgaa ttccgtgagg ccctgggccg atcccacccc
     6961 catgttataa aaaggtcgca tgattcacct ccgctcaggc tgatcgtgtt tcaggttcca
     7021 ggcagcgcgc cagcagaccc gtcgcgtagt cgatgtcgcg ccgccgatgg cgtgcggtga
     7081 tactgaaagt caggtgcgcg ccctcccgcg tgtgcgcgcg ccgtagaatc gttttcacgc
     7141 tggcccgcag cagcgccgcg tgaatacgcc gggcggccga gtccccgact tgaagcgtct
     7201 gcaccgggaa cgggccaccc cggcaggtca cgccgatctc gcgcagcctc cggcgaaagt
     7261 gccggacgcg aagggcgagg cgccttcgca gctcgtcgcc cgactccagg ttgatctcca
     7321 gcgcgcgccg cgcggcgtgc aggacggcga cggaaggcgg gctgcaatga acacgagtct
     7381 cactcaactc ctcgaagccg tgaacggcct cggcgcctcc ggccaagacg gcgagcggga
     7441 cgccgaaagc tttggcgagg gaactgatgg acaggacgag cgggtctcgc aagccgtgcc
     7501 atcgcaaccc gccgccgccg ccgccgccgt acggcgacgc gcgccccggc ccctcgccga
     7561 agatgccgag ggcctgcgtg tcatccaaga tcaaacgtcc gccgaaagag cgcgccgcag
     7621 cgagatactc ggcgagcggc gccagccgtc cgcagtcggg acaaaacccg tcagtcacca
     7681 ccagttgggc ggagcctgca cgcgcgctcc gcagcagcag acggcgcagg tcgtcggggt
     7741 catgatgagc gaaggcgtag acgcgcgccc ccagcgcccg cgcccgctcg actccccaca
     7801 gcgctatggg gtaagtcacc tcgtccacat aaacggtcgc cgggcggccc ccgaacgtcc
     7861 cgaagaagtc ccagaacagg tgcagcgtcg agggggcgag cgtggcgctc tcgcagcctt
     7921 gcagcagcgc cagccgcctt gaaacctctg ccgcgcccgg gggccgagtg agcgccgcag
     7981 gcgccccggt cgtcagttgc tcccacgccg ggacgcaccg gctcgggtgt cgcagtccga
     8041 ggtagagggc ggaggcgaag tcgagcgagg ggctgacgcc cctcaccggt tgcggttgcg
     8101 caccgccagc gactcgcccg cctgtccggc ggacactccc gggccgagcg ccctccggtc
     8161 gcccggcggc agcgccccgc cgccacgctg ctgcgccaga cggttgcgca ggtggatcga
     8221 gggctggacg taacgctcgt cggcgcggcc tgagtccacc agctccaccc cgaggtcaac
     8281 gccggtgacg gcccggtagg cgtggatgta acgctggatt tcgggccgcc agtacctggc
     8341 ccagttggtc gccgcggcca tgtcgttcac ctgggtccag ttgccgtagc ggaccgagag
     8401 cacaatctgc tcgccgttca cggccaggtc gtggaagtac gtgaccgagg agtccatcca
     8461 cccgaacatc gacttgacgg tgtccacgcg gtccatccac ggctcggtgt aggccaccat
     8521 gggcttgccg ccgaggaact cgcgcatctc gggccgcgcc agcagccact gctcgatgag
     8581 catctcctgc cgcgcgttcg agggcaggtc gccgaactgg ttgtgcgccc cgtaagacag
     8641 aatcaggtga acctcccgca gggcgttgag cacctcgaaa ccgtcggcga tgatcgtcgt
     8701 gtcgtcgtcc cgctcgtaga agagggcggc gcggtagagc aggttgtgga acgcttcgag
     8761 gaacttcgag cggctgtcca cggggtcgaa gttctgcacc gccttgccgt agagccgcag
     8821 gccgtactgg tggtcgtact cgtaggcccg gcgcagcacg cccagccggt gctgctcgtc
     8881 ctgcacgtag ccccagagga agttgctcag cccgcgcagc gggtcgatgt cgaaatgcgc
     8941 gagcgggtcg cggttgccgg gcacgatccg cttgttctgg aagcggtagc tgatggcgtt
     9001 catcgtttgg gccagcatcc cctcttcgtg ccagtaggac cagatcagct cgatcatgca
     9061 cggcgcggtg aaccgggagc ggaggatgcc gtagcagtcc acgccgggga cggcgtccgc
     9121 cggcttgatc gccagctcgg ccagccgctg gcggatgagg tcgaagtagg ggagggcttg
     9181 cgcgttctcc gccttcagat cggcgaggta tgcccgccgc agaccttcga gttccgtgcg
     9241 cgtcaccggg cgcccgagcc gcgccgactc ctccgtctcc tgtcgagaga tcaagagcgg
     9301 gtcggtcgtg ttctcgaaga ccaccccgca ctcctgcatg aggaacatct ctgtggcggc
     9361 cttgagaacg gcgtaggccg cgacgccggg gaagggcagg cgcagatcga agccagcacg
     9421 cccttgcagc gggttggcgc acagtaagtt gtcgatgaac tgcttgtagt tcaggaagcc
     9481 gatggccgtc gtgcggtcgc ggatgaccga ccagagcacc tggtcgcccg tgacctgaga
     9541 tgccgcgcgg ttgaggctga tcgagccgct caccggcacg gtcggccctt gcaggacggg
     9601 cggcaggtca tcggcggtca gcagttcctg cgtgacggtg atgtcggcat catccctcgc
     9661 ctgcaccggc ccctctccgt cggcggtgaa catctcgacc cgcgcccgga cgccccgcgg
     9721 cgtgttgtcg gtgaccgtcc acaccgccgc gttgggttcg cccgtcagct ttttgagctc
     9781 cacctcggtc gctgaagcag ggtcgggctg aatccagaac cggaagctgt gcgcgttgat
     9841 atcatcgtcg ccaggctcgg ccaccagctt gaccactccg cctccggtga tcctgtccgg
     9901 caacgccttt agcgtgactt catagtggta ttcgccgttc gcagccttgg cctccgctaa
     9961 tttcttactc atctcgtcct cctcatcatt gtaaagatgg tcctcggtcc gtagcctgag
    10021 actggcagcc gcaacggtac ggctgtgcgt gaccggcgga cgcgttaagt gtgctgcgca
    10081 gcgcccagta ggtgagcagg taggcgagca tcctgctctc ccgctcgggg ctgagtctgc
    10141 cctcggcccg cgcttgcccg agcacggcga aggcgagcga gggcggcgtt tcgcgcatca
    10201 gccgcgggga tcgtcgccac gcgctgaagt aggccgacag cctcgccggt tcgcgctcgt
    10261 gcaccgccat tgcctcccgt agcgaggccc cccgcaacag tctcgggcga tggttgacga
    10321 gcagcgtcac gaagctgggg atgccggctt cgagcgcgac gagcagcgag cgggtcgtcg
    10381 catccaactc ctcaagcggg tagaaggact cccagagctg tgccatccgc tcccactgtc
    10441 tgtgcgggta gagggccttg cctatcgcgc agctgagctt gacgcgaatc cacgggaagg
    10501 ggtgcgggtc gtcgaggttg acgcgaaaga cgaaggcgct cggcaagctg acgacaccca
    10561 tcagcccgac cgtcgcgccg acgccgatgc gcgccacgct cc
//
LOCUS       DBNGOH010000931         2623 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_689236, whole genome shotgun sequence.
ACCESSION   DBNGOH010000931 DBNGOH010000000
VERSION     DBNGOH010000931.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2623)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2623)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2623
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_689236"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..716
                     /locus_tag="AC159P_18200"
                     /pseudo
     CDS             1..716
                     /locus_tag="AC159P_18200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006449515.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
     gene            796..>2623
                     /locus_tag="AC159P_18205"
     CDS             796..>2623
                     /locus_tag="AC159P_18205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697431.1"
                     /translation="MLRASLLLIVMLAPHLAHARAAFAQPVAKTTGTTAANGTVDAAA
                     LSAEITDFLGAEVAAHLADIKSLDPPPARVVGAGATGDYTWGTFMRAVAAYAELSGKR
                     THAGRDLAREVGRIGLLEHRLRSTRFSQLYAVLALRHFGRDLDTNAVWQSLDEGERAQ
                     WRKLLDVSAFYDPKKQQVINLPENYLGVAARIASISYQLGLLKDRALLDGVITRAAHP
                     FTDSGGSLYADDAPPTGRFDRYSNEYARFVWDAAEAAGRKDILDALRPTLNEQMHLWW
                     DLVQPDGYGYAWGRSLGAISYMDTMEIVAFLARHAEFRPAPLEQLSSAYYQAWRWLRQ
                     DYKDKTHLLSVFAHGRGNYAYITREREWQQTTGFFGKAALAHKLFSEALARENLKSFP
                     AEIARPDLARFVFFRQGPRQAGVWLVREGPLHFTLPITTGTRPGVADYLPAPHGLPGF
                     AAPVEQVYPAFTSFIELADGRVLATADGADEIKVENGGRTVRAFWRRWAVVGGKSGEL
                     ADPGITSEVVWLLNGTTLVREEAFTSSQDVTLRRLRFAVPTTAAHHRVRFEEGRRRDL
                     LESSEGTLEVRASVDLPFDVSLAAPGDGALGRGARGAVPLHLI"
BASE COUNT          393 a          920 c          901 g          409 t
ORIGIN      
        1 ttctgctcta ccgcaagccc gaagagcacc cgcgactgtc caaagctgaa ctggcctaca
       61 tccagtccga cccgcccgac ccggtggcga agacgccctg ggcgcgcctg cttccgcacc
      121 gccagacgtg ggccttcgcc atcggcaagt tcatgaccga cccgatctgg tgggtctacc
      181 tcttctggct gcccgacttt ctgaacaagc agcagggcct gagcctgtag gacttcggcc
      241 cgccgctcgt cgtcatctac gtcatcgcgg acatcggcag catcggcggc gggtggctct
      301 cgtcggcgct catcaagcgc gggtggagcg tcaacgcggg gcgcaagacg gcgatgctcg
      361 tctgcgcgct gtgcgtcgtg cccatcgtct tcgcctcgca gaccaagagc ctgtggacgg
      421 cggtggtgct catcggcatc gcggcggcgg cgcatcaggg gtggtcggcg aacatcttta
      481 cgatggcttc ggacatgttc ccgcggcagg ccgtcggctc ggtggtcggc atcggcggca
      541 tggcgggcgc ggtgggcggg atgctgatcg cgcggctcgt cggcgagatt ttgcagcgga
      601 cggggagcta cgtgcccatc ttcatcatcg ccggctcggc ctacctcgcg gcgctgctgg
      661 tgattcacct gctcacgccg cggctcgaac cggcggaggt cgaggccgaa gcatgagcat
      721 gagtttcggc gcggcggagc ttgaaggcgg agcgcccgcc gccgtacgcg gcgggacgcg
      781 cgggtgatgc gaaagatgtt gagagcctcg cttcttttga tcgtgatgct cgcgccgcac
      841 ctcgcgcacg cgcgcgcggc cttcgcgcag cccgtcgcaa agacgacagg gacgacggcg
      901 gcgaacggca cggtggacgc ggcggccctg agcgcggaga tcacggactt cctcggcgcc
      961 gaagtggccg cgcacctcgc cgacatcaag tcgctcgacc cgccgcccgc gagggtggtc
     1021 ggcgcgggcg ccacaggcga ttacacatgg ggcaccttca tgcgcgcggt cgcggcttac
     1081 gcggagctgt cgggcaagcg cacgcacgcg ggccgcgacc tcgcgcgcga agtcgggcgc
     1141 atcggcctgc tggaacacag actcaggagc acgcgcttct cgcagctcta cgcggtgctc
     1201 gccttgcgcc acttcgggcg cgacctcgac accaacgccg tctggcagag cctcgacgag
     1261 ggcgagcgcg cgcagtggcg caaactcctc gacgtgtcgg ccttctacga cccgaagaaa
     1321 cagcaggtca tcaaccttcc cgagaactac ctcggcgtcg ccgcgcgcat cgccagcatc
     1381 agctaccagc tcgggctgtt gaaagaccgc gctctcttgg acggcgtcat cacgcgcgcg
     1441 gcgcacccgt tcacggacag cggcggctcg ctctacgccg acgacgcgcc gccgacgggc
     1501 cgcttcgacc gctactcgaa cgagtacgcg cgcttcgtct gggacgcggc ggaagccgcc
     1561 gggcgcaaag acatcctcga cgcgctgcgt ccgacattga acgagcagat gcatttgtgg
     1621 tgggacttgg tgcagcccga cggctacggc tacgcgtggg gccgcagcct cggcgcgatc
     1681 agctacatgg acacgatgga gatcgtggcc ttcctcgcgc gccacgccga gttccggccc
     1741 gcgccgctcg aacaactctc aagcgcctac tatcaggcgt ggcgctggct gcgccaggac
     1801 tacaaggaca agacgcacct gctctcggtc ttcgcccacg ggcgcggcaa ctacgcctac
     1861 atcacgcgcg agcgcgagtg gcagcagacc acgggcttct tcggcaaggc ggcgctcgcg
     1921 cacaagcttt tcagcgaagc gctcgcgcgc gagaacctca agagcttccc ggcggagatc
     1981 gcgcggccag acctcgcgcg cttcgtcttc ttccggcaag ggccgcggca ggcgggcgtg
     2041 tggctcgtcc gcgaggggcc gctccatttc actctgccca tcacgacggg gacgcgtccg
     2101 ggcgttgcgg actacctgcc cgcgccgcac ggcctgcccg gcttcgccgc gccggtcgag
     2161 caggtctacc cggccttcac ctccttcatc gagctggcgg acggtcgcgt gctcgcgacc
     2221 gccgacggcg cggacgagat caaggtcgag aacggcgggc gcacggtgcg cgccttctgg
     2281 cggcgttggg cggtcgtcgg cggcaagtcg ggcgagctgg ccgacccggg catcacgagc
     2341 gaggtcgtct ggctcctcaa cggcacaacg ctcgtgcgcg aggaagcgtt cacttcgtcc
     2401 caagatgtca cgctgcgccg cctccgtttc gccgtgccga ccaccgccgc gcaccaccgc
     2461 gtccgcttcg aagaggggcg gcggcgcgac cttctcgaat cgagcgaggg cacgctcgaa
     2521 gtgcgcgcgt cggtggactt gccgttcgac gtttctctcg cggcgccggg cgacggcgcg
     2581 ctcggtcgcg gcgcgcgcgg ggccgtgccg ctgcatttaa tct
//
LOCUS       DBNGOH010000932         2842 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3074096, whole genome shotgun sequence.
ACCESSION   DBNGOH010000932 DBNGOH010000000
VERSION     DBNGOH010000932.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2842)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2842)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2842
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3074096"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1088
                     /locus_tag="AC159P_18210"
     CDS             <1..1088
                     /locus_tag="AC159P_18210"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011307436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HIR3697432.1"
                     /translation="RVEKAPAGPARPQTPKGPFPYDAEEVSYENRAAGVRLAGTLTLP
                     RAKGPHPAVLLISGSGQQDRDETILGHKPFHVLADYLTRRGVAVLRFDDRGVGGSAAG
                     PDLNKATSEDFAGDVAAGVEFLKARKEIDPKRIGLVGHSEGGMIAPMVASRSADVAFI
                     VLMAGPGIPAEEGFPAQVSAIARANGVGEEAVAWRLRLLREVFAVMKEERDDAAALRK
                     VRERRAKLLSGLSADERKRLGLADDEMEGVVRVMLTPWFRFFNAYDPRATLKKVRVPV
                     LALTGERDLQVAPAGAHLKAIAEALKAGGNKDYTIMELPKLNHLFQAGETGSPAEYGR
                     IEETISPAALELIGDWILKHAGASPGK"
     gene            complement(1806..>2842)
                     /locus_tag="AC159P_18215"
     CDS             complement(1806..>2842)
                     /locus_tag="AC159P_18215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010038699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697433.1"
                     /translation="LLAVGLTLAAHTPPARAQEPATVTGRVTGPGVGHPPVVNVFVRG
                     QGGDDTVSFLAYPQGFTGGVRVAVGDVSGDGVPDIVTAPGPGIATHIKVFDGASLRKM
                     QSRMGHFMAYPGDFQGGVFVAAGDVNGDGRADIVTGPGEGAPPHVKVFDALTGKTLHS
                     FLAYDRSFLGGVRVSAGDVDGDGVAEIITGAGPGAGPHVRVFSGRDSSADGPTLQHSF
                     FAYDNFAGGVFVAAGDINNDGRDEIITGAVAGGAPHVKAFDALTGNTLHSFFAFDPGF
                     AGGVRVATADVNGDGFYDLIVGAGPGAGPHVRVFDARNNLNPLHDFFAYSPNFLGGVY
                     VGGSSAPRAR"
BASE COUNT          502 a          936 c          993 g          411 t
ORIGIN      
        1 gccgcgtcga gaaggccccg gccggcccgg cgcggccgca gacgccgaag ggccctttcc
       61 cgtacgacgc cgaggaggtc tcctacgaga acagggcggc aggggtgcgc ctcgccggga
      121 cgctgacgct gccgcgcgcg aagggcccgc acccggcggt gctgctcatc tccggctccg
      181 gccagcagga cagggatgag accatcctcg gtcacaagcc cttccacgtc ctcgcagact
      241 acctgacgcg ccgcggggtg gcggtgctcc gcttcgacga caggggcgtg ggcggctcgg
      301 ccgccgggcc ggacttgaac aaggccacga gcgaggactt cgcgggcgac gtggcggcag
      361 gcgtcgagtt cctcaaggcc aggaaggaga tcgacccgaa aaggatcggg ctcgtcgggc
      421 acagcgaggg cggcatgatc gcgccgatgg ttgcgtcgag gtccgcggac gtggccttca
      481 tcgtcctcat ggccgggccg ggcatcccgg ccgaggaggg cttccccgcg caggtctcgg
      541 ccatcgccag ggcgaacggc gtcggcgagg aggccgtcgc gtggaggctc aggcttctgc
      601 gggaggtctt cgccgtcatg aaggaggagc gcgacgacgc ggccgccttg cggaaggtgc
      661 gcgagcggcg ggcgaagctt ctctcggggt tatcggcgga cgaaaggaag cggctcggct
      721 tggcggacga cgagatggag ggggtcgtca gggtgatgct cacgccctgg ttccgcttct
      781 tcaacgccta cgacccgcgc gcgaccctga agaaggtgcg cgtccccgtc ctggccctca
      841 caggcgagag agaccttcag gtggcgccgg ccggggcgca tctgaaggca atcgcggaag
      901 ctctcaaggc cggggggaac aaagactaca ctataatgga gttgccgaag ctcaaccacc
      961 tgttccaggc gggcgagacg ggctcgccgg ccgagtacgg gaggatcgag gagacgatct
     1021 ctcccgccgc cctggagctg atcggcgact ggatcctcaa gcacgccggg gcgagtcccg
     1081 gtaagtaggc acgggagaac tacgcaggcc cgacgttagc ctaagttagg ggaatgacgg
     1141 ctgccgggtg agcacgactg ggacggcttg cacactccgc ggatgtgcct aacacgcacc
     1201 cttcatagga gagtcggcag tcgatgaagc acgccgcgtg cacgcgtagg tgcggctagt
     1261 taacacccgg gaccaagttg taaatagatc gttcgggcac acgtccgtct ccttcgtgaa
     1321 cccatattgt taggtaacac cgcgaccaca aacgcggctc tgatgaagag taatttcagg
     1381 agattaagct catgaaggtc aaatgactgg cagggccgcc cgacaatggg ctgaagcgga
     1441 cgcgccgtca gcaagctttc cattgatctg cgtcgtagcg cgccgcttag cccaagcgtt
     1501 ccctcgtccg ggccttcgtg cggcgacccc acgagatgga ggagattcac gtcaccaacc
     1561 gagtaaggcc gtgagttcgg gtcgcgaccg cccgcctcgt ctacggcaac ggccgcgatc
     1621 aacaacgctg cgggggctat tccccgcagg agggacacat ctgagtaaga gggtcagcag
     1681 gggtcgaact tggcgcgccg taacgtgccg cttgatcaac ctcagccagc ggacccggta
     1741 cggcgcgcgt cgacgcccca aaggttcgcg caccagcccg tccggcatca ggcgctgtaa
     1801 gcgcgtcagc gcgctcgcgg ggcggaggac ccgcccacgt agacgccgcc gaggaagttc
     1861 gggctgtagg cgaaaaagtc gtgtagcggg ttcaggttgt tgcgcgcgtc gaacacccgc
     1921 acgtgcgggc ctgcgcccgg cccggcaccc acgatgagat cataaaagcc gtccccgttg
     1981 acgtcagccg tcgccacccg cacgccgccg gcgaaaccgg ggtcgaaggc gaaaaagctg
     2041 tgcagcgtat tgccggtcag cgcgtcgaac gccttcacgt gcggcgcgcc cccagcgacg
     2101 gccccggtga tgatctcgtc ccgcccgtcg ttgttgatgt ccccggcggc cacgaagacg
     2161 ccgccggcga agttgtcata cgcgaagaag ctgtgctgta aggtagggcc gtccgcactg
     2221 gaatcccggc cactgaagac ccgcacgtgc gggcctgcgc ccggccccgc gccggtgatg
     2281 atctcggcca ccccgtcgcc gtccacgtcg ccggccgaca cccgcacgcc gccgaggaac
     2341 gaacggtcgt aagcgagaaa gctgtgcagc gttttaccgg tcagcgcgtc gaacaccttc
     2401 acgtgcggcg gcgcgccctc gccggggccg gtgacgatgt ctgcgcgccc gtcgccgttg
     2461 acgtcgcccg ccgccacgaa gacgccgccc tgaaagtccc cggggtaagc catgaagtga
     2521 cccatccggc tctgcatctt gcgcagagaa gcgccgtcga agactttgat gtgcgtcgcg
     2581 atgcccggcc cgggtgcggt aacgatgtcg ggcacgccgt cgccgctgac gtcgccgacg
     2641 gcgacgcgga ctcccccggt gaacccctgc gggtaggcga ggaaggaaac ggtgtcgtcc
     2701 ccgccctgcc cccgcacgaa gacgttgacg accggcgggt gccccacgcc cggcccggtg
     2761 acgcgcccgg tgacggtcgc cggttcctgt gcccgcgcgg gcggggtgtg ggcggcgagg
     2821 gttaaaccga ccgctaataa ta
//
LOCUS       DBNGOH010000933         2267 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3990959, whole genome shotgun sequence.
ACCESSION   DBNGOH010000933 DBNGOH010000000
VERSION     DBNGOH010000933.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2267)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2267)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2267
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3990959"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..740
                     /locus_tag="AC159P_18220"
     CDS             <1..740
                     /locus_tag="AC159P_18220"
                     /EC_number="2.4.1.117"
                     /inference="COORDINATES: protein motif:HMM:NF012745.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004581 - dolichyl-phosphate
                     beta-glucosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dolichyl-phosphate beta-glucosyltransferase"
                     /protein_id="HIR3697434.1"
                     /translation="VIVVDDGSRDETVGVSEEVFAAARGRTEATVIRVEPNRGKGHAV
                     RTGLLAARAPVALFSDADLSTPITETPKLVAPIRTGQYDVVFGSRALDRSLIGVHQPW
                     RREQSGRVFNLVMRLATGLPYWDTQCGFKAFRTDVCRPVFEGALIDRFGFDVELLYIA
                     HKANLRLLERAVRWNDVEGSKVGLMTGLQGFNELREVRRNDKRGLYDEAIRRTREAAA
                     RARAERPTLLEEEAESDASPELAAQRI"
     gene            985..1749
                     /locus_tag="AC159P_18225"
     CDS             985..1749
                     /locus_tag="AC159P_18225"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013257772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HIR3697435.1"
                     /translation="MTARLNINIDHVATVRQARRAPEPSVVAAAVLCEQAGADGITVH
                     LRGDRRHIQDEDIYTLRRAVTTYLNLEMAATEEMLLVALDARPDAVTLVAERPEEITT
                     EGGLNVSENFDTVRGAVDRLREGGQFVSLFIDPDPRQIEASLSAGAQQIELCTAAYAE
                     ATLGARAVRDEGRARAAEELERLRAAAALAARSGLHVAAGHGLTYRNVAAVASIPEIV
                     EFNIGHNVVARALFAGLAEAVREMRARIREGTDPKG"
     gene            1982..>2267
                     /locus_tag="AC159P_18230"
     CDS             1982..>2267
                     /locus_tag="AC159P_18230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697436.1"
                     /translation="MRQPLRTRRLPSFVAAGLLFLLAPAALRAQPTPPPQPAPAAGQM
                     RLAKVEFTGLQRVKPEEALAASGLAAGQNVDINAVDAAAEKLLQSGLFMRL"
BASE COUNT          379 a          831 c          709 g          348 t
ORIGIN      
        1 aggtcatcgt cgtggacgac ggctcgcgcg acgagaccgt cggcgtctcg gaagaggtct
       61 tcgccgccgc gcgcgggcgc accgaggcca ccgtcatccg cgtcgaaccg aaccgcggca
      121 aggggcacgc cgtccgcacg gggcttttag ccgcgcgcgc gccggtcgcg ctcttctccg
      181 acgccgacct ctcgaccccg atcacagaga ctcccaagct cgtcgccccc atccgcaccg
      241 gccagtacga cgtggtcttc ggctcgcgcg cgctcgaccg ctcgctcatc ggcgtccacc
      301 agccgtggag gcgcgagcag agcgggcgcg tcttcaacct cgtcatgcgc ctcgccacgg
      361 gactgccgta ctgggatacg cagtgcggct tcaaagcctt ccgcacggac gtgtgccgcc
      421 ccgtcttcga gggcgcgctc atcgaccgct tcggcttcga cgtggagctg ctctacatcg
      481 cgcacaaggc gaacctgcgc ctgctcgaac gcgccgtgcg ctggaacgac gtggaaggga
      541 gcaaggtcgg attgatgacc ggcctgcaag gcttcaacga gctgcgcgag gtgcgtcgca
      601 acgacaagcg cggcctctac gacgaggcca tccgacgcac acgcgaggcc gccgcccgcg
      661 cccgcgccga aagacccacg cttcttgaag aagaggccga gagcgacgcc tcgcccgaac
      721 tcgccgcgca gagaatctga aagcagtcgg cagtaggcgg taggcagtag gcagtcgaag
      781 caagaggctt gagcggcaac gtctcaagcc tcttgcttca ttgcatgaag agcggagagg
      841 cgttgcactt tacctcccaa gcctctcggc tcttcgcctg ttttgactgc ctaccgccta
      901 ccgcctactt cttaattggc ccgattcggc gggatgaaag tggctcttcc ggcgcggccc
      961 gttttcgcct atcctctgct gaccatgacc gcccgtctca acatcaacat cgaccacgtg
     1021 gcgaccgtcc ggcaggcgcg gcgcgcgccg gagccgagcg tcgtcgcggc ggctgtgctc
     1081 tgcgagcagg cgggggccga cggcatcact gtgcacctgc gcggcgaccg ccgccacata
     1141 caggacgagg acatctacac gctgcgccgc gccgtcacga cctatctgaa tttggagatg
     1201 gcggcgaccg aggagatgct cctcgtcgcg ctcgacgcgc ggcccgacgc cgtgacgctc
     1261 gtcgccgagc gccccgaaga gatcacgacc gagggcggcc tcaacgtctc cgagaacttc
     1321 gacaccgtgc gcggcgccgt tgaccgcctg cgcgagggcg gccagttcgt cagcctcttc
     1381 atcgaccccg acccgcgcca gatcgaagcc tcgctgagcg caggcgcgca gcagatcgaa
     1441 ctctgcaccg cggcttacgc cgaggcgacg ctcggcgccc gcgcggtgcg cgacgaaggg
     1501 cgcgcccgtg ccgccgaaga actcgaacgg ctgcgcgccg ccgccgcgct cgccgcgcgc
     1561 tccggcctgc acgtcgccgc cggccacggc ctcacctacc gcaacgtcgc cgcggtcgcc
     1621 tccatccccg agatcgtcga attcaacatc ggccacaacg tcgtcgcgcg cgccctcttc
     1681 gccggactcg ccgaagcggt gcgcgagatg cgcgcacgca tccgcgaagg caccgacccg
     1741 aaggggtaaa aggggaagag gagaaaaggc gaagaagcga aaagggaagg gcaagaggga
     1801 ggatgagaag cagcgtgcct ctcatcctcc ctcttcccct tttcccttct tcccctttac
     1861 ccctcgctcc tctggcagct accccgccgc ttcgagcatc tattaaatat ctctcggccc
     1921 ggcctttcat ccgccgaata aaatgggagc agcctccgtg acgcttttcc gaactcctcg
     1981 aatgcgacaa ccgcttcgca cccggcgcct tccgtccttc gtcgccgccg gtcttctctt
     2041 tctgctcgcg cccgcggctt tgcgcgcgca gcccacgccg ccgccgcagc ccgcccccgc
     2101 cgccgggcag atgcgtctgg cgaaggtcga gttcacgggg cttcagcgcg tgaagccgga
     2161 ggaggcgctc gccgcgagcg gtctcgccgc ggggcaaaac gtggacatca acgccgtgga
     2221 cgcggcggcg gaaaaacttt tgcagtccgg cctcttcatg cggctcg
//
LOCUS       DBNGOH010000934         2535 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1058741, whole genome shotgun sequence.
ACCESSION   DBNGOH010000934 DBNGOH010000000
VERSION     DBNGOH010000934.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2535)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2535)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2535
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1058741"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..325)
                     /locus_tag="AC159P_18235"
     CDS             complement(<1..325)
                     /locus_tag="AC159P_18235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697437.1"
                     /translation="MTTATEEKVQSGGANEQTPAPEAVPTKGAEAMAPERRETFFPSR
                     LAENPFAFMRRFAEDMERFFEDFGSFRIKPLLRGELMPRVSEFGRAVWAPEVEFFERD
                     GQFTVR"
     gene            657..1208
                     /locus_tag="AC159P_18240"
     CDS             657..1208
                     /locus_tag="AC159P_18240"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HIR3697438.1"
                     /translation="MTREECYAVLAAAGFGRLACARDNQPYVVPVYFAFDGSHIYGCE
                     GPHVYSFSAPGQKIEWMRANPLVCLEIDDVKSQNEWTSVVVFGKYEELPDTPNYQHAR
                     EHAHELLQKRASWWEPACVTVAHHAGPQPFAPVYYRISVERLTGHRAVPDPNEAAAMN
                     VPTSHGKEGGWLKHLLHLPRSEG"
     gene            complement(1220..1732)
                     /locus_tag="AC159P_18245"
     CDS             complement(1220..1732)
                     /locus_tag="AC159P_18245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697439.1"
                     /translation="MRLLNIIFILVLMPSTVAAQGVTFAREGVEYRLELPSSRWRAVE
                     RLDVHDHFDFVHGEDRDDGYLRVRKSLVGAGTTPKDLHLEDEARTLKLLPGYVACSTC
                     EGEKFSGALSGAVFSYEYTSGGLPMAGRVYYLRVDARTYYTLHFTCNRKWIADVSVQA
                     DSIARSFGLK"
     gene            complement(1765..2445)
                     /locus_tag="AC159P_18250"
     CDS             complement(1765..2445)
                     /locus_tag="AC159P_18250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007979458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697440.1"
                     /translation="MRKSVLSSAALAAAGVTAAAAYVYTVRPWHLKWGATEEETSERL
                     PGDELVPRPNVEATHAVTIEAPVKDVWPWLVQIGQDKGGFYSYTWLENLVGCHMRNAG
                     RIMPECQQLGVGDSVRLHPKAPPLPVLICEPPRALVLGSNTDSPGTWGFYLKEVDEDT
                     TRLVVRGRGEWKPGLLSWLGAYVVFEPAHFIMERKMLLGIKQRAEALRQCVGAGSRRR
                     VRVAGEQW"
BASE COUNT          437 a          848 c          804 g          446 t
ORIGIN      
        1 cgcggacggt gaactgcccg tcgcgctcga agaactcgac ctccggcgcc cacaccgcgc
       61 gcccgaactc cgagacgcgc ggcatcagct cgccgcgcag gagcggcttg atgcggaagc
      121 tgccgaagtc ctcgaagaac ctctccatgt cctcggcgaa gcggcgcatg aaggcgaagg
      181 ggttctcggc caggcgcgac ggaaagaacg tctcgcgacg ctccggggcc atcgcttcgg
      241 cgcccttcgt cggcacggcc tccggcgcgg gcgtctgttc gttcgcgccg ccgctttgca
      301 ctttctcttc tgttgctgtc gtcatctctt ttctccttgt cgggcaaccg ccagcccttg
      361 cggggtcggg gttgcacgca gttgtcaccc gcctagtttc aggcttccga ggtgcaactg
      421 ttatgccttc gaaggaagag tcgaagacgc gatgaaaagg tttgatcgag ccgcgcgtga
      481 gtctgcgagc ggaaaactac gcactcccgc gagtaatttc acgcacgggt cgcaggccgg
      541 tctaagtcgc ggagcggttt atgcggtggt tcgtatggaa tgcccgttgc accttcggac
      601 ggcaagggac tcaagggcaa ctcactacgc ggaggcgcat catgttgatc gaggagatga
      661 caagggaaga atgttacgcg gtgctcgcgg cggcgggttt cggcaggctg gcgtgcgcgc
      721 gtgataacca gccgtacgtc gtccccgtct atttcgcctt cgacggcagc cacatctacg
      781 gctgcgaggg gcctcacgtt tactctttct ccgcccccgg gcagaagatc gagtggatgc
      841 gcgccaaccc gctggtctgc ctcgaaatcg atgacgtgaa aagtcagaac gagtggacga
      901 gcgtcgtcgt cttcggcaag tacgaggagc tgccggacac acctaattac cagcacgcac
      961 gcgagcacgc tcacgagcta ctgcaaaagc gtgcgagttg gtgggagccg gcctgtgtca
     1021 cggtcgcgca ccacgccggc ccacagcctt tcgcgcccgt ctactaccgc atcagcgtcg
     1081 agcggctgac cggacaccgc gccgtgcccg acccgaatga agcggcggcg atgaatgtgc
     1141 cgacgagcca cggcaaagaa ggcggctggc tgaagcacct gcttcatctt ccgagatcgg
     1201 aaggttgaaa cggtcgccgt catttcaatc cgaaactgcg ggcgatcgag tcggcctgca
     1261 cgctcacgtc ggcgatccat ttccgattgc acgtgaagtg cagtgtgtaa taggtgcgcg
     1321 cgtcgactcg caggtaatag acgcgccccg ccatcggcag gccgccgctc gtgtattcgt
     1381 aggagaagac cgcgccgctc aacgcgcccg agaacttctc gccctcgcac gtcgagcacg
     1441 cgacgtaacc gggcagcagc ttcagcgtcc tcgcctcgtc ttcgaggtgc aaatctttcg
     1501 gtgtggtgcc cgcccccacc agactcttgc ggacgcgcag gtatccgtcg tcgcggtctt
     1561 cgccgtggac gaaatcgaag tggtcgtgca cgtcgaggcg ctcgacggca cgccaccggg
     1621 acgagggcag ttccagtcgg tattcaactc cctcccgcgc gaacgtgacg ccctgcgcgg
     1681 cgacggtcga aggcatcaag acgaggatga agatgatgtt tagaagccgc atcgcacacg
     1741 ccctcccttc gcctccggct gcgctcacca ctgctcgccc gccacgcgga cacgccgcct
     1801 gctaccagcc ccgacgcact gccggagcgc ctcggcgcgt tgcttgatgc cgagcagcat
     1861 cttccgctcc atgatgaagt gagccggctc gaacaccacg taggcgccga gccaactgag
     1921 cagacctggc ttccactcgc cgcgcccgcg cacgacgagg cgcgtcgtgt cctcgtccac
     1981 ctctttgagg tagaagcccc atgtgccggg gctgtccgtg ttgctgccga gcaccagcgc
     2041 ccgcggcggt tcgcagatga ggacgggcag cggcggcgct ttgggatgca gccgcaccga
     2101 atcgccgacg ccgagctgct ggcactcggg catgatgcgg cccgcgttgc gcatgtggca
     2161 gccgacgagg ttttcgagcc acgtgtagct gtagaacccg cccttgtcct gcccgatctg
     2221 cacgagccac ggccagacat ctttcacggg ggcctcgatc gtgacggcgt gcgtcgcctc
     2281 gacgttcggg cgcggcacca gctcgtcgcc cggaaggcgc tcgcttgtct cctcttcggt
     2341 cgcgccccac ttgaggtgcc acgggcgcac ggtgtaaacg taagccgccg cggcggtgac
     2401 gccggccgcc gccagtgcgg cggaactcaa aacactcttt ctcatttctc tccccttccg
     2461 gcgtcagccc gcggcgaggc ccggctcgtt catcctccgc gacagcagcg cgtggagcag
     2521 gaacaggaag ccggg
//
LOCUS       DBNGOH010000935         3417 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2889966, whole genome shotgun sequence.
ACCESSION   DBNGOH010000935 DBNGOH010000000
VERSION     DBNGOH010000935.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3417)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3417)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3417
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2889966"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..458)
                     /locus_tag="AC159P_18255"
     CDS             complement(<1..458)
                     /locus_tag="AC159P_18255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697441.1"
                     /translation="MKDVVRSGSSVADATPQRTFILDPALKGRATFNRCHAAKSERRL
                     CGEINDKLCPDMPNNMRLLIRLFVVLIVICETAPAQSAWPRPVPARVKDGENRDLFVM
                     TLGDVETTLADGTFDPLRDEVRLKDGTVKPNYYKEALRVKYFRPMDKSRFA"
     gene            complement(574..2514)
                     /locus_tag="AC159P_18260"
     CDS             complement(574..2514)
                     /locus_tag="AC159P_18260"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 97 protein"
                     /protein_id="HIR3697442.1"
                     /translation="MTMNTRRRQKACTRRAFLSVAVAAPALAASLPRAGAAMNGDVMK
                     VVSPDGRVRFELVGRGAPRLGFRVTLRGRPVIETSPLGIVIDGVDLSEGAEVVRVETY
                     RVREKYPWRGVHSEAVDHCHGARILVTHAAGKTAYTVEARAYNDGVAFRHVVPGGEKQ
                     RVPDEATAFKVPAGSVVWFHDFEGHYEGVHARKSIAEVKPGEWAAPPLTVKLPGGAGY
                     ASITEAALVNYAGMGLQADGARGFKAVLGHALPISYPFKLRYGDEEGKRLSQPAAITG
                     TITTPWRVVLAGADLNTLVNSDIVHNVSPPPDRTLFPKGFDTEWVRPGRAVWKYLDGG
                     ENNLETAKEFSLLAGRLGFEHNIVEGFWRNWSEAEMRELVEYSKRQGVGVWFWKHSRD
                     LRTPEKRREFFELCRRVGVVGAKIDFLDHEAKEVIDHYQALLRGAAEHRLMVNFHGAN
                     KPAGEARTWPNELTREGVRGLEYRRAETRATHNTTLPFTRLLAGHADYTPVIFGERRR
                     ETSWAHQIATAAVFTSPVLIYGAHPRTMLEHPAVEMLKSLPAAWDETRVLAPSEIGEV
                     ALFARRRAATWFLAVLNGAAARTLRVPLSFLGGGKHQALVVRDRMDEAAAVRIENTTA
                     VKNDALTMELRAGGGFIARFQR"
     gene            complement(2544..>3417)
                     /locus_tag="AC159P_18265"
     CDS             complement(2544..>3417)
                     /locus_tag="AC159P_18265"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF017263.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HIR3697443.1"
                     /translation="TNPDNFTEHRRYLMRLSVQVPALAAAWELTKDGRYAEHAARHLR
                     AWFIDPSTRMNPHLEYAQAVPGVNTGRGIGLIETRGLTRVVDAVGLLVGSKAWTREDQ
                     RGLEEWFAKFLKWMQESRHGREEAAAKNNHGTYYDIQTVSFALFIGQRELAKNILQAA
                     REKRVAAQIEPDGSQPLELQRTRAWSYSVGNLDGLLVLAALGERVGIDLWNYRTRDGR
                     GLRRALEFLYPFAVGEKKWAHPQITEWSPQSLFPLMRRAAARYRDEKFRQMMSRVPAA
                     DPASRERLVQSGGM"
BASE COUNT          510 a         1163 c         1137 g          607 t
ORIGIN      
        1 gcgaaacgcg acttgtccat cggcctgaaa tactttacgc gcagcgcctc tttgtagtag
       61 ttcggcttga cggtgccgtc cttcagacgc acctcgtccc gcaaggggtc gaacgtcccg
      121 tcggctaaag tagtctcgac atcgcccagc gtcatcacga acaggtctct attctcgccg
      181 tccttgaccc gcgcaggcac ggggcgcggc cacgccgact gcgccggggc tgtctcgcat
      241 atcacaatca gaacgacgaa gagacggatg agcaatctca tgttgttcgg catgtccggg
      301 caaagcttat cgttgatttc gccgcaaagc ctgcgctctg atttcgccgc gtggcagcga
      361 ttgaatgtag cccggccttt caaggccggg tcaaggatga aggtacgctg gggcgtcgcg
      421 tcagcgacgc ttgaacccga ccgcaccaca tccttcatgc gtcgctgacg cgacgcgggt
      481 tcgagttgtc atcgccgacc cggcgttgaa aggccgggct acattcaagc gccgctacgc
      541 gacggaatac gcacgtcgtc tctcgcacgt tcgctaacgc tggaaccgcg cgatgaaccc
      601 gccgcccgcg cgcagctcca tcgtgagcgc atcgttcttg acggcggtcg tgttttcgat
      661 cctgaccgcc gctgcttcgt ccatccggtc gcgcacgacg agcgcctgat gtttgccgcc
      721 gccgaggaac gagagcggga cgcggagcgt gcgcgcggcg gcgccgttga ggacggcgag
      781 gaaccaggtg gcggcgcggc ggcgcgcgaa gagggcgacc tcgccgatct cggaaggcgc
      841 gagcacgcgc gtctcgtccc acgcggcggg gaggctcttc aacatctcga ccgcagggtg
      901 ctcaagcatc gttcgcgggt gcgcgccgta gatgagcacg ggcgaggtga agacggcggc
      961 ggtcgcgatc tggtgcgccc acgaagtctc gcggcggcgc tcgccgaaga tgacgggcgt
     1021 gtagtccgcg tggcccgcga gcaggcgcgt gaaggggagc gtcgtgttgt gcgtcgcgcg
     1081 cgtctcggcg cggcggtatt cgaggccgcg gacgccctcg cgcgtcagct cgttgggcca
     1141 cgtgcgcgcc tcgcccgcgg gcttgttggc gccgtggaag ttgaccatca gcctgtgctc
     1201 ggcggcgccg cgcaggaggg cttgatagtg gtcgatcacc tccttcgctt cgtggtcgag
     1261 aaaatctatc ttcgcgccga cgacgccgac ccgccggcac agctcgaaga actcgcgccg
     1321 cttctcgggc gttctgaggt cgcggctgtg cttccagaac cagacgccga ccccctgccg
     1381 cttcgagtat tcgaccagct cgcgcatctc ggcctcgctc cagttgcgcc agaagccttc
     1441 gacgatgttg tgctcgaagc cgagccgtcc ggcgaggagc gaaaactctt tcgccgtctc
     1501 aaggttgttc tcgccgccgt cgaggtactt ccacacggcg cggcccggcc tcacccattc
     1561 ggtgtcgaag cctttcggaa agagcgtcct gtcgggcggc ggcgagacgt tgtgcacgat
     1621 gtccgagttg accagcgtgt tcaagtccgc gcccgccagc acgacgcgcc acggcgtggt
     1681 gatggtgccc gtgatggccg ccggttggga gagccgcttc ccttcctcgt cgccgtagcg
     1741 cagcttgaac gggtagctga tcggcagggc gtggcccaag accgctttga agccgcgcgc
     1801 gccgtccgct tgcagaccca tgcccgcgta gttcacgagc gcggcctccg tgatcgaagc
     1861 atagcccgcg ccgccgggca gcttcaccgt cagcggcggc gcggcccact cgcccggctt
     1921 cacttcggcg atgcttttgc gcgcgtggac gccttcgtag tggccctcga agtcgtggaa
     1981 ccagacgacg ctccccgccg gaaccttgaa cgcggtcgcc tcgtccggca cgcgctgctt
     2041 ttctccgccg gggacgacgt ggcggaaggc cacgccgtcg ttgtaggcgc gcgcctcgac
     2101 ggtgtaggcg gtcttgccgg cagcgtgtgt gacgaggatg cgcgcgccgt ggcagtgatc
     2161 gacggcctcg gaatgcacgc cgcgccacgg atacttttcc cgcacgcggt acgtctcgac
     2221 cctgaccacc tccgcgcctt cggacaagtc aacgccgtcg atgacgatgc cgagcggcga
     2281 cgtttcgatc accggcctgc ccctcagcgt gacgcgaaag ccgaggcgcg gcgccccgcg
     2341 cccgacgagt tcgaaccgca cgcgaccgtc ggggctgaca accttcatca cgtcaccgtt
     2401 cattgcggcc ccggcgcgcg gcaggctcgc ggccagcgcc ggcgccgcga ccgccaccga
     2461 gaggaacgcg cggcgcgtgc aagccttctg acggcggcgt gtgttcatgg tcatgtgcgt
     2521 ctcgctttcg tctcccgccc gcgtcacatg cctccggact gaaccagtcg ctcgcggctc
     2581 gcagggtcgg cggctgggac tctggacatc atctgccgga acttctcgtc ccggtatctc
     2641 gccgccgcgc ggcgcatcaa agggaagagc gactgcggac tccattcggt gatctgcggg
     2701 tgcgcccact tcttttcgcc gacggcgaac gggtagagga attcgagcgc gcgacgcaag
     2761 ccacgaccgt cgcgcgtccg gtagttccag agatcgatgc cgactctctc gccgagcgcg
     2821 gcgagcacca acagcccgtc gaggttgccg acgctgtagc tccacgcgcg cgtccgttga
     2881 agttcgagcg gctggctccc gtccggttcg atctgcgcgg cgacgcgctt ctcgcgcgcg
     2941 gcctgcaaga tatttttcgc caactctctc tggccgatga agagcgcgaa cgagacggtc
     3001 tggatgtcgt agtaggtgcc gtggttgttc ttcgcggcgg cctcttcgcg cccgtgcctg
     3061 ctctcctgca tccacttcag aaacttcgcg aaccactctt cgaggccgcg ctggtcttcg
     3121 cgcgtccaag ccttcgagcc tacgagcagg ccgaccgcgt ccaccacgcg cgtgaggccg
     3181 cgcgtctcga tcagaccgat gccgcggccc gtgttgacgc cggggacggc ctgcgcgtat
     3241 tcgaggtgcg ggttcatccg cgtcgagggg tctatgaacc acgcgcgcag gtggcgcgcg
     3301 gcgtgctcgg cgtagcgccc gtccttcgtc aactcccacg cggcggcgag cgcgggcacc
     3361 tgcacgctga ggcgcatcag gtagcgacga tgctccgtga agttgtcggg gttcgtc
//
LOCUS       DBNGOH010000936         3500 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_689784, whole genome shotgun sequence.
ACCESSION   DBNGOH010000936 DBNGOH010000000
VERSION     DBNGOH010000936.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3500)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3500)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3500
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_689784"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1496
                     /locus_tag="AC159P_18270"
     CDS             <1..1496
                     /locus_tag="AC159P_18270"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF013640.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HIR3697444.1"
                     /translation="IKATLIATLQTPPSPDGRELAALPAAVDWLEVRADLVGDIDADW
                     LRSRFAGRLLYALRSRAEGGHFADACEQRHERLRRAACSYDMVELEAERDFAPELLDD
                     IDADKRLVAWHGAAAAADLAARFEQISAVPARLYKLVTHAAKCGDELAPLSLLGSLGR
                     ADTVAYASGPLGFWTRLVAPRLGAPIVYGLAPGGPLVPAEPTINKLIEDYGLPQLSIV
                     EGIYGIVGNPVFHSLSPRLHNAAYRETGHPALYVPLRVESFGEFWREVVNAGVLESLH
                     MPFKGLTVASPHKEAALLFAKTASPMARRAESTNILVRNNGSWKADTTDPEVAFMAQR
                     GRGGRARQQRAAVIGCGGAGRAIATALAEGHTEVTLVHRGAERGHYAAQLLDLPYIPL
                     PGFSAEGFDIVVNATPVGRDDGDAPFEVETLSEDAVVIDLVYGSQPTPLVAGAVAHGR
                     TAMDGREVLLAQVLRQFQLMVGREMPAPLVREQLGFEAEPSDLIAAG"
     gene            1592..2773
                     /locus_tag="AC159P_18275"
     CDS             1592..2773
                     /locus_tag="AC159P_18275"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013334368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR3697445.1"
                     /translation="MACDRQALIDRARELAREKFAPRAAHYDRTASFPAEDFADIFEA
                     GLLAPVVPEEHGGLGLGPYRGDVFTLWMMTKEFAKADLSLARCWEGHVNSLVLLDGMA
                     TPEQKAAWFKGVVERGDKWVAWSGEPQARAPGETVRFGTTTEKVEGGYVVDGNKVFST
                     SAGGAQWAILLVNTAGPGGVRHAAGSPDTQLLMACDLSDPTVETDGSWWDPIGMRGTV
                     SYLVNFRRTFIPDANLIGYPGQYLKEGWQTCFIPHYAATFTGAAEAAYEYALRYVTTQ
                     KKTEDPYIQHHVGQMSVDVETAHLWLRHVARLWETGRYSEAQLAGSRARHVIEHLATG
                     TVEHAIRACGARCLNRPSALERIYRDLSFYVRHDNDDQILATVGKSILGLTHDPSFYK
                     P"
     gene            2789..>3500
                     /locus_tag="AC159P_18280"
     CDS             2789..>3500
                     /locus_tag="AC159P_18280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697446.1"
                     /translation="MKLTTAKFIHLGLDLGCQWSYQMASGKRRFTLTIWWLALGYFVF
                     YAPYLGFIKATVGGLWPGVGAGVSGFRLLPAVVISTAVVLTVIVTLKGWWKHAGRCRV
                     FGLSVPFPRPLVFLSGLGTAIIIGTTTLIFTFSGLSIVFALVLMRGGVLIMGPVVDRV
                     FGRRVRWFAWAAFSVTVPAILIALADVNNYSLSALTAAVIVSYLAGYLLRIPCATWLA
                     KSRDEATTYRYFVEEQLVA"
BASE COUNT          512 a         1254 c         1174 g          560 t
ORIGIN      
        1 tgattaaagc aactctcatc gcgaccttgc agacccctcc ttcgccggac ggtcgagaat
       61 tggcggcgct gcccgcggcg gtcgattggc tggaggtgcg cgccgacctc gtgggcgaca
      121 tcgacgccga ctggctccgc agccgcttcg cggggcggct gctctacgcg ctgcggagcc
      181 gcgccgaggg cggccacttc gcggacgcgt gcgaacagcg gcacgaacgg ctgcgccgcg
      241 ccgcgtgctc ttacgacatg gtcgagcttg aggccgagcg cgatttcgcc cccgagttat
      301 tggacgacat cgacgccgac aagcgactcg tcgcgtggca cggggccgcc gccgccgcag
      361 acctcgcggc ccgcttcgag caaatctccg ccgtccccgc aaggctttat aagctcgtca
      421 cgcacgccgc gaagtgcggc gacgagctgg cgcccctgtc tctgctcggc tcgctcgggc
      481 gcgcggacac ggtcgcctac gcgagcggcc cgctcgggtt ctggactcgc ctcgtcgcgc
      541 cgcgcctcgg cgcccccatc gtctacggcc tcgcgcccgg cgggccgctc gtccccgctg
      601 agccgaccat caacaagctc atcgaggact acgggctgcc gcagctctcg attgtcgaag
      661 gaatctacgg catcgtcggc aaccccgtct tccactcgct ctcgccgcgc ctgcacaacg
      721 cggcctacag ggagacgggc caccccgcgc tctacgtgcc gctccgcgtc gagtcgttcg
      781 gggagttctg gcgcgaggtg gtcaacgccg gggtgctcga atcgctgcac atgccgttca
      841 aggggctgac ggtcgcctcg ccgcacaagg aggccgcgct gctcttcgcc aagaccgcca
      901 gcccgatggc gcggcgcgcg gagtccacca acatcctcgt ccgaaacaac ggctcgtgga
      961 aggccgacac gaccgacccc gaggtcgcct tcatggcgca gcgcgggcgg ggcgggcgcg
     1021 cgcggcagca gcgcgcggcg gtgatcggct gcggcggggc gggccgcgcc atcgccacgg
     1081 cgctcgccga aggccacacc gaggtcacgc tcgtccaccg cggggccgag cgcggccact
     1141 acgccgcgca gctcctcgac ctgccttaca tcccgctgcc cggcttcagc gccgagggct
     1201 tcgacatcgt cgtcaacgcg acgccggtcg ggcgcgacga cggcgacgcg ccgttcgagg
     1261 tcgagacgct gagcgaagac gccgtggtca tcgacctcgt ctacggctcg cagccgacgc
     1321 cgctggtcgc gggcgccgtg gcgcacgggc gcacggcgat ggacgggcgc gaggtgttgc
     1381 tggcgcaggt gctccggcag tttcagttga tggtcggtcg ggagatgccc gcgccgctgg
     1441 tgcgcgagca gctcggcttc gaggccgagc cttccgactt gatcgccgcg ggctagcaga
     1501 gccttcgcgc gcgacacgac ttttccgcac gaaccgacga gactcttcga cacgaacccg
     1561 aggaggtggc cccatcgaaa cgcaactcga gatggcctgt gatcgccaag ctttgatcga
     1621 ccgcgcgcgc gagctggccc gcgagaagtt cgcgccgcgc gccgcccact acgaccggac
     1681 ggcctccttc cccgcggagg acttcgcgga catcttcgag gcgggcctgc tcgcgccggt
     1741 cgtgcccgaa gagcacggcg gcctcggcct cggcccgtac cgcggcgacg tgttcacgct
     1801 gtggatgatg acgaaggagt tcgccaaggc cgacctctct ttggcacgtt gttgggaagg
     1861 gcacgtcaac tcgctcgtcc tgctcgacgg gatggcaacg cccgagcaga aggccgcgtg
     1921 gttcaagggg gtcgtcgagc gcggcgacaa gtgggtcgcg tggagcggcg agccgcaggc
     1981 gcgcgcgccg ggcgagacgg tgcgcttcgg gacgacgacc gagaaggtcg agggcggcta
     2041 cgtcgtcgat ggcaacaagg tcttctcgac gagcgcgggc ggcgcgcagt gggcgatcct
     2101 gctggtcaac acggcggggc cgggcggcgt gcgccacgcc gccggctcgc ccgacacgca
     2161 gctcctcatg gcctgcgacc tctccgaccc gaccgtcgaa acggacggct cgtggtggga
     2221 ccccatcggg atgcgcggca ccgtcagcta cctcgtcaac ttccgccgca cgttcatccc
     2281 cgacgcgaac ctcatcggct accccggcca gtatctgaaa gagggctggc agacctgctt
     2341 catcccccac tacgccgcga ccttcacggg cgccgccgaa gccgcctacg agtacgcgct
     2401 tcgctacgtg acgacgcaga agaagacgga agacccttac atccagcacc acgtcggcca
     2461 aatgtctgtg gacgtggaga cggcgcacct gtggctgcgg cacgtcgcgc ggctgtggga
     2521 gacgggccgg tacagcgagg cgcagctcgc gggcagccgc gcccgccacg tcatcgaaca
     2581 tctggcgacg ggcacggtcg agcacgccat ccgcgcctgc ggcgcgcgct gcctgaaccg
     2641 cccgagcgcg ctcgaacgca tctaccgcga cctctctttc tacgtccgcc acgacaacga
     2701 cgaccagatt ctcgccacgg tcggcaaatc gatcctcggc ctcacgcacg acccgtcctt
     2761 ctacaagccc tgacgaaggc gggcggcctt gaagttaacg acggcgaagt tcatccacct
     2821 cggcctcgac ctcggttgtc aatggagtta tcaaatggct tcgggtaaac gtcgcttcac
     2881 tttgaccatc tggtggctgg cgctcggcta cttcgtcttc tacgcgcctt acctcggttt
     2941 catcaaagcc acggtcgggg gcttgtggcc gggcgtgggc gcgggcgtct ccggcttccg
     3001 cctgctgccc gccgtggtca tctcgacggc ggtcgtgctg accgtcatcg tcactctcaa
     3061 ggggtggtgg aagcacgcgg gccgctgccg cgtcttcggg ctgagcgtcc cgttcccgcg
     3121 cccgctggtc ttcctctcgg gcctcggcac ggccatcatc atcgggacga cgacgctgat
     3181 cttcaccttc tccggcctct ccatcgtctt cgcgctggtg ctgatgcgcg ggggcgtgct
     3241 catcatgggg ccggtcgttg accgcgtctt cgggcgtcgc gtccgctggt tcgcgtgggc
     3301 ggccttctcg gtcacggtgc ccgcgatact gatcgcgctc gccgacgtta acaactacag
     3361 cctgtccgcc ctgacggcgg cggtcatcgt ctcttacctc gcgggctacc tgctgcggat
     3421 tccctgcgcc acgtggctgg cgaagtcgcg cgacgaggcg accacttacc gctacttcgt
     3481 cgaagagcag ttggtcgcgg
//
LOCUS       DBNGOH010000937         2484 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1242201, whole genome shotgun sequence.
ACCESSION   DBNGOH010000937 DBNGOH010000000
VERSION     DBNGOH010000937.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2484)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2484)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2484
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1242201"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(92..1564)
                     /gene="gatB"
                     /locus_tag="AC159P_18285"
     CDS             complement(92..1564)
                     /gene="gatB"
                     /locus_tag="AC159P_18285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HIR3697447.1"
                     /translation="MKNGWEAVIGLEIHAQLSTESKIFCGCSTRFGDEPNANTCPVCL
                     GLPGSLPVLNRRVVELGARAALALGLRINEVSIFARKNYFYPDLPKGYQISQYDRPFS
                     ADGVLEVMTAARDEGGHAREWRPMTVRITRLHLEEDAGKNVHEGLPETERFSYIDLNR
                     AGTPLAEIVTEPDFRTSWEAYDYVNHVRRALQWVGASEADMEKGNLRCEANVSVRKIG
                     AEKFGTKVELKNLNSVRFMQRAIEYEIERQIELIERGGRVTQETRLWDERAGATRVMR
                     SKEEAHDYRYFPEPDLQPLVVAADFVERVRAEMPELPEARRQRFMAEYELSFADASLL
                     TRDRALADYYERAASASNARGASNWLKSELLRELDAANLGADASPVSPEELGALVRAI
                     EEGKISGKQGKDVLVEMFRTGKAASAVIEEQGLAQVSDAGEIEKIITDVLASNHAQVE
                     QYRAGKEGLFGFFVGQVMKVSKGKANPKVVNELLREKLKP"
     gene            1792..2211
                     /locus_tag="AC159P_18290"
     CDS             1792..2211
                     /locus_tag="AC159P_18290"
                     /inference="COORDINATES: protein motif:HMM:NF025683.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4383 domain-containing protein"
                     /protein_id="HIR3697448.1"
                     /translation="MAKTIATVLGVAFLVVGVLGFIAPGLAGFHLSLAHNLVHIISGA
                     LALYFGLKGTLAAARLFCIVFGIVYGLLGVVGYALGTDRPHTLPGVTHGGTDSNLLQV
                     IAGQLELASMDHGLHVLLGLVFLVGGLLTKANVRDAN"
BASE COUNT          397 a          867 c          739 g          481 t
ORIGIN      
        1 ccatcacaac ctcaaaggcc gtgaacagta agccgtaaac cgtgacaggt gaagaactgc
       61 tttcttcttg tcacggttta cggcttactg ttcacggttt tagtttctcc ctcagcagct
      121 cgttgacgac cttcggattc gccttgcctt tcgagacctt catcacctgc ccgacaaaga
      181 agccgaacag cccttccttg cccgcgcggt actgctcgac ctgcgcgtgg ttggaggcga
      241 gtacgtccgt gatgatcttt tcgatctcgc ccgcgtcgct gacctgcgcg aggccctgct
      301 cctcgatgac ggcggaggcg gccttgcccg tccggaacat ctcgacgagc acgtccttgc
      361 cctgcttgcc gctgatcttg ccttcctcga tcgcgcgcac gagcgcgccg agttcttcgg
      421 gcgagaccgg cgaggcgtcc gcgccgaggt tcgccgcgtc caattcgcgc agcagctccg
      481 acttcagcca gttcgacgcg ccgcgcgcgt tcgacgccga ggccgcgcgc tcgtagtagt
      541 ccgccagcgc gcggtcgcgc gtcagcagcg acgcgtccgc gaacgacagc tcgtactcgg
      601 ccatgaagcg ttggcgccgt gcttcgggca gctcgggcat ctccgcgcgc acgcgctcga
      661 cgaagtccgc cgcgacgacg agcggctgca agtcaggctc cgggaaatag cggtagtcgt
      721 gcgcctcttc cttcgagcgc atcacgcgcg tcgcgcccgc gcgctcgtcc cacagccgcg
      781 tctcctgcgt gacgcgcccg ccccgttcga tcagctcgat ctgccgctcg atctcgtact
      841 cgatggcgcg ctgcatgaag cgcacggagt tcaggttctt cagctcgacc ttcgtgccga
      901 acttctcggc gccgattttc cggacggaga cgttcgcctc gcagcgcagg ttgcccttct
      961 ccatgtcggc ctcgctcgcg ccgacccact gcaacgcgcg ccgcacgtgg ttgacgtagt
     1021 cgtacgcctc ccaagaggtg cggaagtccg gctccgtcac gatctcggcg aggggcgtcc
     1081 ccgcgcggtt gaggtcgatg taggagaagc gctcggtctc gggcagcccc tcgtggacgt
     1141 tcttgcccgc gtcctcttcg aggtgcaggc gcgtgatgcg gacggtcatc ggtcgccact
     1201 cgcgcgcgtg cccgccctcg tcgcgcgccg ccgtcatcac ttccagcacg ccgtccgccg
     1261 agaacggtcg gtcgtactgc gagatttgat agcccttcgg caagtccggg tagaaatagt
     1321 tcttgcgcgc gaagatcgag acctcgttga tgcgcaggcc cagcgccagc gccgcccgcg
     1381 cgcccagctc caccacccgc cggttcagca ccggcagcga gccgggcagc cccagacaca
     1441 ccgggcacgt gttcgcgttc ggctcgtcgc cgaaccgcgt cgagcacccg cagaatatct
     1501 tcgactcggt gctcagttgc gcgtgaatct caaggccgat gacggcctcc caaccgtttt
     1561 tcatagaagt cggaagtcag gagtcagaat gatgggcaag tatagcatcg ccatcgacgc
     1621 gcgctcaacg cgggtcgatt ccgccgccgc ctcgtaaaaa cccggccacg ggccgcgccc
     1681 acgtgttgta caatcggccc gccaaccctt ttgctggccg gcggcgttcg gtttcggcgg
     1741 cggcgcacgg ctgcacgcgt tcggcttctc ttccaaagga gtgcaaagaa catggcgaag
     1801 acaatcgcga ccgtactcgg cgtggcgttc ctggtggtgg gcgtgctcgg cttcatcgcg
     1861 cccggcctcg ccggcttcca cctcagcctc gcgcacaacc tcgtccacat catctcgggc
     1921 gcgctcgccc tctacttcgg cctcaagggg acgctcgccg cggcacgcct cttctgcatc
     1981 gtgttcggca tcgtctacgg cctgctcggc gtcgtcggct acgcgctcgg caccgaccgg
     2041 ccacacacgc tccccggcgt cacgcacggc ggcaccgact cgaacctgct ccaggtgatc
     2101 gccggccaac tggaactcgc ctcgatggat cacggcctgc acgtcctgct cggcctcgtc
     2161 ttcctcgtcg gcggactgct gacgaaggcc aacgtcagag acgccaacta acggagaggg
     2221 gaaaagggga aaaggggaag aggcgaaaag ggagaatgac aagcaccgtg tctctcatca
     2281 tcgagttgat gagaagcagc gcgcctttcg tcttccctct tccccttttg accttttacc
     2341 cttttccacc tcttcttcct tttgaccttt tcccctcttc ccgtaagatg catcacttcg
     2401 gaagccgatt catagggaga cacttgttgc catgatcaag acggtggagt ggactgcgga
     2461 gggcgtggtg atgatcgacc agcg
//
LOCUS       DBNGOH010000938         8651 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4906623, whole genome shotgun sequence.
ACCESSION   DBNGOH010000938 DBNGOH010000000
VERSION     DBNGOH010000938.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8651)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8651)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8651
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4906623"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            600..2234
                     /gene="lnt"
                     /locus_tag="AC159P_18295"
     CDS             600..2234
                     /gene="lnt"
                     /locus_tag="AC159P_18295"
                     /EC_number="2.3.1.269"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HIR3697449.1"
                     /translation="MAASPNAPARRVRGIRSLVSRAGSAAPTRAETLLSVLSAALLVL
                     AFPDFELWPLAWVGLIPLLFAIGRRAHAGRAFLLGWMTGTLFFYASCYWLTHPMIHYA
                     GIPAWLAYPLFLPAALAGGLFPALCACMLARACVRWGARALLLAPFVWVSLEWARLGV
                     VGQLWNALGYSQAYVPVLIQGARFGGVYLVGFLVVCVNAALAYALLEHGRRAVLTSAG
                     ALAAVALAVAGFDLAPPPLVEREPRALVVAVQPNVIPDFERPAAETARLAQEHFELSA
                     RALDALDKDQPDGSLPRVVVWPESPMNFSFARDARFREQAADFARARRASLLFNSLEP
                     APGGGAYNAAVLVNEEGRLAVQYDKIRLMPFGEYVPLPRWLPGAGMVRGLVGEFTPGT
                     RYTLMPLGGSDAGAPRAGVFICLESAYPHITRQFAGEGADVLIEITNDAYQGDTAILR
                     QHMANAVFRAVETGRPLLRVTNTGITARIDERGRVLDEAGKNRQEVRTWPVARSDDEK
                     TFYVKWGDGFVAVCSLVSLALLLLTYVTSRRRAVGG"
     gene            2247..2462
                     /locus_tag="AC159P_18300"
     CDS             2247..2462
                     /locus_tag="AC159P_18300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697450.1"
                     /translation="MLDKLVPAATKWVFLAGLGVLVGIVYLLLYKGAELLLELAVGEK
                     PGGWYGGAFFLSSLVVAFLLTRRRRPS"
     gene            2516..3626
                     /gene="prfB"
                     /locus_tag="AC159P_18305"
     CDS             join(2516..2587,2589..3626)
                     /gene="prfB"
                     /locus_tag="AC159P_18305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_319006089.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HIR3697451.1"
                     /translation="MIETFTIQELRTRVEELGRRVEQLGGFFDVAAKREELARIESQA
                     SAQDFWSDQEAAQRLLRQRSRLERVIARQEGFESSIADAAVLFEFAEEDEESLRELRE
                     LVERLEHEVDQAETEMLLSGESDALNAICTIHPGAGGTESQDWAEMLLKMYLKWAERR
                     GFKTEVLDYQPGEEAGLKSVTFRVEGEYAYGLLSAEAGVHRLVRISPFDQAARRHTSF
                     ASLFVSPEIDENIEVEVQDKDLRIDTYRATGAGGQHINTTDSAVRITHLPTGIVVACQ
                     NQRSQHQNRAVAMQVLRSRLYELELEKRRAETAVLEASKADISFGSQIRNYVLAPYRL
                     VKDARTKHERGDVDAVLGGDLDDFIHSYLLSRRAA"
     gene            3877..6513
                     /locus_tag="AC159P_18310"
     CDS             3877..6513
                     /locus_tag="AC159P_18310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014786796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE family DNA translocase"
                     /protein_id="HIR3697452.1"
                     /translation="MKMPGGVGPRRSRRGEIVTVVLVAASALLALCLVSYSPNDASWS
                     AAGGDGASNLIGAVGANVAAALFQSFGLAACMLPLLLAAAAWRRINAPLSRVAGLLTI
                     TLAAASLCALFITSPIADRSFNAGGFVGALVAESLRGVLNTVGAGVLLSALAAIGLLL
                     ATNFSFVGAYERIAAALANPSGSFRVTLERYRSWREARREQAHARAEMRREAREARER
                     EIRESLQLAPLPSASEAAHPTISAKRAATEGVRAASAVRRAANAAPAAEKLSPEDEVR
                     AKLAKAEAELAAIADSAHNPPPAHEAAPPPPPIITPKAKRVVSPPPPSAPPPVEETWR
                     EAPDVEEMLRTAAVVRTGFDDEEEFPSEVAPPAAAPAKKREEKKAFTPALLDYDLPEH
                     AFLVAPPPRHEQADEELLQIATRLAEKCKEFNVTGQIKHICPGPVVTTYEFKPDPGVK
                     YSRVTGLVDDLCIALEAESVRIDRLPGKPHVGIEVPNPERETIHLREVIESAKYRDSA
                     SKLTLALGKTIDGMNYVADLARMPHLLIAGATGTGKSVCINSLLVSILYKARPDEVKF
                     ILIDPKRLELGLYADIPHLATPIITDPKRASNALKWAVGQMEQRYKDLARWGVRNIDG
                     FNAEVERRNNVLDFDEDGLPHKSLPYIVIIIDELADLMMTCGNDVEESITRLAQMARA
                     VGIHLVLATQRPSVDVITGLIKANFPSRIAFRVSSKVDSRTIIDSNGAEALLGRGDML
                     FLPPGTSRLVRVHGAFLDEAEVNRIVAHIKSQSAPVYDETITQSEEEASGGEGGSGGE
                     RDELFEQALRICCEMKRASTSVLQRRLRIGYGRAAAILDAMEREGYIGQADGARPRPV
                     LGKSFETVSHWDGLQEESPEDY"
     gene            6734..7081
                     /locus_tag="AC159P_18315"
     CDS             6734..7081
                     /locus_tag="AC159P_18315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697453.1"
                     /translation="MSSTPTDGRFMRCYKGVLTDEGVECQAFRSEDGELFTLLGDLGG
                     FSDGDKVVVCGTIAEISPCMQGTTLVISFIGKKAPRAKKSVAASARAKTGSSAKKAAK
                     SSKPSKSSGRKSR"
     gene            7401..7709
                     /locus_tag="AC159P_18320"
     CDS             7401..7709
                     /locus_tag="AC159P_18320"
                     /inference="COORDINATES: protein motif:HMM:NF024245.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HIR3697454.1"
                     /translation="MPRRTDLAAHDVSPRATQTAASHQPEPAPPSTHVNDATTPVSIN
                     LAPREELERLPGVGPALAARIVEHRERHGPFRRLEHLLLVRGISERRLRELRPFITTQ
                     "
     gene            complement(7816..>8651)
                     /locus_tag="AC159P_18325"
     CDS             complement(7816..>8651)
                     /locus_tag="AC159P_18325"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HIR3697455.1"
                     /translation="GQGDSALVTMPDGTTLLVDGGGQPRFGARATEASEGDGGEPFER
                     DARGVGEAVVSEYLWRRGLDRVNYVVATHADADHIDGLADVLRNFRTDAALVARAPAA
                     DEEFERFAATAAEKGTPLYLVGRGDTLRFGGATVEVLWPPRAADTAAAARRPSRNEDS
                     LVLRLSLGSRAFILTGDIEADAESRLVSSSASRLRCDALKVAHHGSRTSSTQRFADAA
                     RPSIAVISVGLDSPYGHPRPEVVERWRAHGARVLTTGERGTITVSTDGADLKVETFLK
                     P"
BASE COUNT         1399 a         2971 c         2833 g         1448 t
ORIGIN      
        1 taacagaagt cggaagtcgg aagtcagaag aaagaaagac gattgacgag gaggcttggg
       61 gcagtagctc tcaagcctct tcccttttct gacttccgcc ttccgacttc cgacttccga
      121 cttatgattt gtggcacgcg aactgcttta aggaaagtcg gcaagcacgg acgaagttaa
      181 accgtgaccc cgccgccgtc acttctctga gcgtggcctg ccgctacgcc agccgcccgc
      241 cgagcgaccc aatcttaacc cccagccttt caagaacctg gcccccagcc aggttctcct
      301 gctttttggg cttttttatt tttccgcctt cgatgcgacg cttccggcgt cgcccgccgg
      361 ggacaaacct gtcaattcgc ggcgacaaat cgcctgtcgg cgacattcct tcccgccgcc
      421 gtcgcccgtc actgcgacaa aaaatgtcac cggaggggtg acgatttctg ttgatgtccg
      481 accttttacg ttagccgcgc agacgatatg acgcacgccg gaaatttttt cgcggcgcgt
      541 ccgatgacgg cgatgatgac ccggaatcgt cgcccctctg ttaaactcgc cgcgtgcaga
      601 tggccgcgtc gcccaacgcg cccgcgcgca gagtgcgggg catcaggtca ctcgtcagcc
      661 gcgcggggag cgccgcgccg acacgcgcgg agaccctgct gtcggtgctc tccgcggcgc
      721 tgctcgtcct cgccttcccc gacttcgagt tgtggccgct cgcgtgggtc gggctgatcc
      781 cgctgctctt cgccatcggg cgacgagcgc acgcgggtcg cgccttcctg ctcgggtgga
      841 tgacgggcac gctcttcttc tacgcctcct gctactggct cacgcacccg atgattcatt
      901 acgcgggcat ccccgcgtgg ctcgcgtacc cgctgttcct gcccgccgcg ctcgcgggcg
      961 gtctcttccc ggcgttgtgc gcgtgcatgc tggcgcgcgc gtgcgtgcgt tggggggcgc
     1021 gtgcgctcct gctcgcgccg ttcgtctggg tgtcgctcga atgggcgcgg ctcggcgtgg
     1081 tggggcaact gtggaacgcg ctcggctact cgcaggcgta cgtccccgtg ctcatacagg
     1141 gcgcgcgctt cggcggtgtg tacctcgtcg gcttcctcgt cgtctgtgtc aacgcggcgc
     1201 tcgcttacgc gctgctcgaa catgggaggc gtgctgtcct cacgtccgcc ggggcgctcg
     1261 cggcggtggc gctcgccgtc gccggcttcg acttggcgcc gccgcccctc gtcgagcggg
     1321 agccgcgcgc gctggtcgtg gccgtgcagc cgaacgtgat ccccgacttc gagcgaccgg
     1381 cggcggagac ggcgcggctc gcccaggagc acttcgagtt gagcgcgcgg gcgctcgacg
     1441 cgttggacaa agaccagccc gacgggagcc tgccgcgcgt ggtcgtgtgg cccgagtcgc
     1501 cgatgaattt ttccttcgcg cgcgacgcgc ggttccgcga gcaggcggcg gattttgcgc
     1561 gcgcgcgccg cgcgtcgctg ctcttcaact cgctcgaacc cgcgcccggc gggggggctt
     1621 acaacgccgc cgtgctcgtg aacgaagagg gccgcctcgc cgtccagtat gataagattc
     1681 gcctgatgcc gttcggcgaa tacgtcccgc tgccgcgctg gctgcccggc gcagggatgg
     1741 tgcgggggct ggtcggcgag ttcacgccgg gcacgcgcta cacgctgatg ccgctcggcg
     1801 gctcggacgc gggggcgccg cgcgcaggag ttttcatctg ccttgagtcg gcctacccgc
     1861 acatcacgcg gcagttcgcg ggcgagggcg ccgacgtgct gatcgagatc acgaacgacg
     1921 cttatcaggg cgacaccgcc atcctgcgtc agcacatggc gaacgcggtc ttccgcgccg
     1981 tcgagacggg ccgcccgctc ctgcgcgtga ccaacaccgg catcacggcg cgcatcgacg
     2041 agcgcggtcg cgtgctcgac gaagccggga agaaccggca agaggtgcgg acgtggccgg
     2101 tcgcgcgctc ggatgatgag aagacttttt atgtgaagtg gggcgacggc ttcgtcgccg
     2161 tttgttcgtt ggtcagcctc gcgttgttgc ttttgactta cgtgacgagc cgccgacgcg
     2221 ccgtcggcgg ctgaaggata aaggccatgc tggataaact cgtccccgcg gcgacgaagt
     2281 gggtcttcct cgcggggctt ggcgtgctcg tcggcatcgt ttacctgctc ctttacaaag
     2341 gcgccgagtt actactcgaa ctcgcggtgg gcgagaagcc cggcgggtgg tacgggggcg
     2401 cgttctttct gtcgagcctc gtcgtcgctt tcctcctgac gcggcgtcga aggccgtcgt
     2461 aataatctgt tcggcgattc gctggccggt cttatcgaat tttcggggga gacacatgat
     2521 cgagactttc acgattcagg aactacgcac gcgcgtcgaa gagttggggc ggcgcgtcga
     2581 acaactcggg aggtttcttt gacgtagcgg cgaagcgcga ggagttggcg cgcatcgaat
     2641 cgcaagcttc ggcgcaggac ttctggtcgg atcaggaggc cgcgcagcgg ctccttcgac
     2701 aacgcagccg cctcgaacga gtcatcgcgc ggcaggaggg cttcgagtcg tcaatcgccg
     2761 acgccgccgt gctcttcgag ttcgcggaag aagacgagga gagcctgcgc gagctgcgcg
     2821 agctggtcga aaggctcgaa cacgaggtcg atcaggcgga gacagagatg ctgctgtcgg
     2881 gcgagtcgga cgcgctgaac gcgatctgca cgatccatcc gggcgcgggc ggcaccgagt
     2941 cgcaggactg ggccgagatg ctgctgaaga tgtacctgaa gtgggccgag cggcgcggct
     3001 tcaagaccga ggtgctcgac taccagccgg gcgaggaggc gggcctcaag agcgtcacgt
     3061 tccgcgtcga gggcgagtac gcctacggcc tgctgtcggc ggaggcgggc gtccaccggc
     3121 tggtgcgcat ctcgccgttc gaccaggcgg cgcggcgcca cacgagcttc gcctcgctct
     3181 tcgtctcgcc cgagatcgac gagaacatcg aggtcgaggt gcaggacaag gacttgcgca
     3241 tcgacaccta tcgggcgacc ggcgcgggcg gccagcacat caacacgacc gattcggcgg
     3301 tgcgcatcac gcatctgccc acggggatcg tcgtcgcgtg ccagaaccag cgctcgcagc
     3361 accagaaccg cgcggtggcg atgcaggtct tgcgctcgcg gctctacgag cttgagcttg
     3421 agaagcgtcg tgccgagacc gccgtgctcg aagcgagcaa ggccgacatc tccttcggtt
     3481 cgcagatacg caactacgtg ctcgcgcctt accggctcgt caaggacgcg cgcacgaagc
     3541 acgagcgcgg cgacgtggac gccgtgctcg gcggcgacct cgacgacttc atccactcgt
     3601 acctgctctc gcgccgcgcg gcgtgataaa gcggacttcg ttcttgccaa agtagagaaa
     3661 aagttgtaag cgtttcctta cgggcgcgct gcttcgactg aaacgccagc cgagcgagcg
     3721 cgccccgccg gcgcaacccc ccgcgcacgt tagtaccact caaccgattg attcgagcaa
     3781 caggtcggtc gtccgccgtg tcttcggcac ggacggctcc ggttgacttg tgcccgcagg
     3841 gagcggttta tggcaactat gcaaagcgat gtgcggatga aaatgccggg gggcgtcggg
     3901 ccgcgcaggt cgcggcgcgg cgagatcgtg acggtcgtgc tcgtcgccgc gagcgcgctg
     3961 ctggcgctgt gcctcgtctc gtacagcccg aacgatgctt cgtggagcgc ggcggggggc
     4021 gacggcgcga gcaacttgat cggcgcggtc ggcgcgaacg tggccgcggc gctgttccag
     4081 tcgttcgggc tggcggcgtg catgctcccg ctgctgctgg cggcggcggc gtggcggcgc
     4141 atcaacgcgc cgctctcgcg cgtcgcgggc ctgctgacga tcacgctcgc cgcggccagc
     4201 ctgtgcgcgc tcttcatcac cagccccatc gccgaccgca gcttcaacgc gggcgggttc
     4261 gtcggcgcgc tggtggccga gtccctgcgc ggcgtgctga acacggtcgg cgcgggcgtg
     4321 ctgctctcgg cgctggcggc catcgggctg ctgctcgcca cgaacttctc cttcgtcggc
     4381 gcctacgagc gcatcgccgc cgcgctcgcc aacccttcag gctccttccg cgtgacgctc
     4441 gaacgctacc gctcttggcg cgaggcgcgg cgcgagcagg cgcacgcgcg cgcagagatg
     4501 cggcgcgagg cgcgcgaggc gcgcgagagg gagatacgcg agtcgctgca actcgcgccg
     4561 ctgccgagcg cgagtgaggc cgcgcacccg accatcagcg ccaagcgcgc cgcgaccgaa
     4621 ggcgtgcgcg ccgcgtccgc cgtccgccgc gcggccaacg ccgcgcccgc cgcggagaag
     4681 ctctcgcccg aagacgaggt gcgcgccaag ctcgctaagg cggaggcgga actcgccgcc
     4741 atcgccgact cggcccacaa cccgccgccg gcacacgaag cggcaccgcc accgccgccg
     4801 atcatcacgc ccaaagcgaa acgggtcgtc tcgcccccgc cgccgagcgc gccgccgccg
     4861 gtcgaggaga cttggcgcga ggcgccggac gtggaggaga tgctgcgcac ggcggcggtc
     4921 gtgcgcacgg gcttcgacga cgaagaggag ttcccttccg aagtcgcacc gcccgccgcc
     4981 gcgcccgcga agaagcgcga agagaagaag gctttcacgc ccgcgctgct cgactacgac
     5041 ctgcccgagc acgcctttct agtcgcgccg ccgccgcgcc acgagcaggc cgacgaggag
     5101 cttttgcaga tcgccacgcg cctcgccgag aagtgcaagg agttcaacgt caccggtcag
     5161 atcaaacaca tctgccccgg ccccgtcgtc acgacctacg agttcaagcc cgaccccggc
     5221 gtcaagtatt cgcgcgtgac gggtctggtg gacgacctct gcatcgcgct cgaagccgag
     5281 tcggtgcgca tcgaccgcct gcccggcaag ccgcacgtcg gcatcgaggt gccgaacccc
     5341 gagcgcgaga cgatccacct gcgcgaggtc atcgagtcgg ccaagtaccg cgactccgct
     5401 tccaagctca cgctcgcgct cggcaagacc atcgacggca tgaactacgt cgcagacctc
     5461 gcgcgcatgc cgcacctgct gatcgcgggc gcgaccggca ccggcaagtc ggtctgcatc
     5521 aactcgctgc tcgtctcgat tctctacaag gcgcggcccg acgaggtgaa gttcatcctc
     5581 atcgacccga agcgccttga gcttggtttg tacgccgaca tcccgcacct cgccacgccg
     5641 atcatcaccg acccgaagcg cgcttcgaac gcgctgaagt gggcggtcgg gcagatggag
     5701 cagcgctaca aagacctcgc gcgctggggc gtgcgcaaca tcgacggctt caacgccgag
     5761 gtcgagcgcc gcaacaacgt gctcgacttc gacgaggacg gcctgccgca caagtcgctc
     5821 ccctacatcg tcatcatcat cgacgagctg gccgacctga tgatgacctg cggcaacgac
     5881 gtggaagagt ccatcacgcg cctcgcgcag atggcgcgcg ccgtcggcat ccacctcgtc
     5941 ttggccacgc agcgcccctc ggtcgatgtc atcaccggcc tcatcaaggc gaacttccct
     6001 tcgcgcatcg ccttccgcgt ctcctcgaag gtggactcgc gcaccatcat cgactcgaac
     6061 ggcgcggagg cgctcttggg tcgcggcgac atgctcttcc tgccgcccgg cacgtcgcgc
     6121 ctcgtgcgcg tccacggcgc cttcctcgac gaagcggagg tgaaccgcat cgtcgcgcac
     6181 atcaagtcgc agagcgcgcc cgtctacgac gagaccatca cgcagtccga agaggaagcc
     6241 tcgggcggcg aaggcgggtc gggcggcgag cgcgacgagc tgttcgagca ggcgttgcgc
     6301 atctgctgcg agatgaagcg cgcctcgacc tccgtgctcc aacgccgcct gcgcatcggc
     6361 tacggtcgcg ccgccgccat cctcgacgcg atggagcgcg agggctacat cgggcaggcc
     6421 gacggcgccc gcccgcgccc cgtcctcggc aagtccttcg agaccgtcag ccactgggac
     6481 ggcctgcaag aagagtcgcc cgaggactac tgaggaaagg ggaaaaggta aaaaggggaa
     6541 gaggggaaaa ggaagagaag ttgcttctcc cttttcccct cttcctcttt ttaccttttt
     6601 gccccttctt cgttgacaac ccgccccgcg cgcgaataac ctgcccgcgt caaaggttcg
     6661 cactcacgcc ggcgagagag ctttaccgcg tcggcctcac acaccgtccg caaccaacgc
     6721 tggagaaaca atcatgagca gcactccgac cgacggcaga ttcatgaggt gttacaaggg
     6781 cgtcctcacc gacgagggcg tcgaatgcca ggcgttccgc tccgaagacg gcgaactctt
     6841 cacgctgctc ggcgacctcg gcggtttcag cgacggcgac aaggtggtcg tctgcggcac
     6901 catcgcggag atttcgccgt gcatgcaggg caccaccctc gtcatcagct tcatcggcaa
     6961 gaaagcgccg cgcgccaaaa agagcgtcgc ggcgagcgcg cgcgcgaaga ccggctcctc
     7021 ggccaagaag gctgcgaagt cttccaagcc ttccaaatcc tccgggcgca agtctcgtta
     7081 agcgcgcgac ctccgcgcgc cgaataaaat ttacgagggg cgtctgtcaa gaacgtcagg
     7141 gcgcccctcg gtttcagccg acaatcttcc gacaactttt ccacagttac tgtggaaaag
     7201 tctgtggata agtcgctttt tcaggttaca agtttctgaa aaatttagac ttctagcatt
     7261 ttgcacacat tcgcggcagg ccgtcacaag ttcttcgcga agaacggata ccgacgatgc
     7321 gtcaaggctt ttgtcaagct ctcttccgta ttttttctca cttcattctc gtcgccgcgt
     7381 taacctgcgc ctgcgtcaaa ttgccccgcc gcacagacct cgccgcccac gacgtttccc
     7441 cgcgcgccac gcaaaccgcc gcctcgcatc agcccgaacc cgcgccgccg tcaacccacg
     7501 tcaacgatgc gacgacgccc gtctcgatca acctcgcccc gcgcgaggag cttgagcgac
     7561 tgcccggcgt cggccccgcg ctcgccgcgc gcatcgtcga gcaccgcgaa cgccacggcc
     7621 ccttccgccg cctcgaacac ctcctcctcg tccgcggcat cagcgaacgc cgcctccgcg
     7681 aactgcgacc cttcatcacg acacagtgag acacaaaagc cgtgaaccgt gaaccgtgac
     7741 gagttaaaac agcatccctc tcaacactcg tcattcgtta ctcgtcaccg cttttcactt
     7801 gtcacggttc accgttcacg gctttaagaa cgtttccacc ttcaggtctg cgccgtcggt
     7861 gctgacggtg atcgtgccgc gctcgccggt ggtcaagacc cgtgcgccgt gtgcgcgcca
     7921 gcgttcgacg acttcggggc gcgggtggcc gtagggcgag tcgaggccga cggagatgac
     7981 ggcgatggag ggacgcgccg cgtcggcgaa gcgttgggtc gaggaggtgc ggctgccgtg
     8041 gtgcgcgacc ttgagggcgt cacacctcag tcggctcgcg gaggacgaga cgagtcgtga
     8101 ttccgcgtcc gcttcgatgt cgccggtgag gatgaaggct ctgctgccga ggctcaggcg
     8161 cagcacgagg gagtcttcgt tgcgcgaggg ccggcgcgcg gcggcggcgg tatccgcggc
     8221 gcgcggcggc cagaggactt cgacggtcgc cccgccgaag cggagcgtgt cgccgcgccc
     8281 gacgaggtag agcggcgtgc ccttctccgc cgcggtcgcc gcgaagcgct cgaactcttc
     8341 gtcggcggcg ggcgcgcgtg cgacgagcgc ggcgtccgtc ctgaagttcc gcagcacgtc
     8401 ggcgaggccg tcgatgtggt ctgcgtcggc gtgcgtggcc acgacgtagt tgacgcggtc
     8461 gagtccgcgg cgccagaggt attcggagac gaccgcctcg ccgacgccgc gcgcgtcgcg
     8521 ctcgaacggc tcgccgccgt cgccctccga cgcctccgtc gcgcgtgcgc cgaagcgcgg
     8581 ttgcccgccg ccgtccacga gcagcgtcgt gccgtcgggc atcgtcacga gcgccgagtc
     8641 tccctgcccc a
//
LOCUS       DBNGOH010000939         2339 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4724110, whole genome shotgun sequence.
ACCESSION   DBNGOH010000939 DBNGOH010000000
VERSION     DBNGOH010000939.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2339)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2339)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2339
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4724110"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..853)
                     /locus_tag="AC159P_18330"
     CDS             complement(<1..853)
                     /locus_tag="AC159P_18330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697456.1"
                     /translation="MNPQSHRATAFVAALLLLMHAAPPAHVAARAQRRTTPSREAVIF
                     IVSKAEGADAQVEPVAFVNNSGALVEPVLTGAAPTSDESEAAALPDLFTRYYRPGAKY
                     RLVFGGAEAGSVTIREAVRAECSPNAARVDIGTTTAKLGGNVMALALVGAYTLRPQSS
                     RRAPTEQERSAAFDVAKSVLAQKRVSASVVGRNTKTLNLTATDLDGDGREELVGSYVV
                     KTGPRVRDTLFLVAEPRGRGYRAALSKYARINAGEMMDPSLIDNIGEGGLGTELFVEQ
                     LDVDGDGV"
     gene            complement(913..>2339)
                     /gene="pruA"
                     /locus_tag="AC159P_18335"
     CDS             complement(913..>2339)
                     /gene="pruA"
                     /locus_tag="AC159P_18335"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - L-glutamate gamma-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - L-proline catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HIR3697457.1"
                     /translation="NPAKKTEVVGRFHKATPELARAAVEKASEAFQTWSRTLPQQRAD
                     LLFRVASLLRERRHYFSAWMVHEVAKTWPEADGDTAEAIDFCDFYAREMLRYAAPQPL
                     VKIAGEENRLTYIPLGVGVVIPPWNFPLAIMAGMTVASVVTGNTVVLKPSSDAPAIAY
                     KFFELLEEAGMPPGVVNFMTGSGAEVGDVVVDHPKTRYVAFTGSKEVGLRINERAAKV
                     NDGQIWIKRVVAEMGGKDAIIVDADTDLDEAATGVVQSAFGFQGQKCSACSRAIIHEA
                     VYEPMLELIAERTARLKVGDPLDPSTTMSAVINNKAFKSISDYIEKGQADGGRLIAGG
                     GSDGEQGFFIEPTVIADVKPGDRIEQEEIFGPVLACIRAADFDEALRIANDTEYGLTG
                     AVYTNDREKLERARREFHVGNLYLNRKCTGALVGVHPFGGFNMSGTDSKAGGRDYLGL
                     FMQAKVVSEKVHPGYNDKEKRDAI"
BASE COUNT          333 a          805 c          790 g          411 t
ORIGIN      
        1 ccacgccgtc gccgtccaca tccaactgct cgacgaaaag ctcggtgccg agaccgcctt
       61 cgccgatgtt gtcaatgagc gagggatcca tcatctcgcc ggcgttgatg cgcgcgtact
      121 tcgagagcgc cgcacggtag ccgcgccctc gcggctcggc gacgaggaag agcgtgtccc
      181 tgactcgcgg cccggtcttg accacgtagc tgccgaccag ttcttcgcgc ccgtcgccgt
      241 cgaggtcggt cgccgtcagg ttgagcgtct tcgtgttgcg tccgacgacg gacgcgctga
      301 cgcgcttctg cgcgaggacg gacttcgcca cgtcgaaggc ggcggagcgc tcctgctcgg
      361 tcggcgcgcg ccgcgagctt tgcgggcgta gcgtgtacgc gccgaccaac gcgagcgcca
      421 tcacgttgcc gccgagcttc gcggtcgtcg tgccgatgtc aacgcgcgcc gcgttgggcg
      481 agcactccgc gcgcacggcc tcgcggatcg tcacggagcc tgcttccgcg ccgccgaaga
      541 ccagacgata cttcgcgccc ggcctgtaat agcgcgtgaa caaatcgggc agcgctgccg
      601 cttcggactc gtccgaggtc ggcgcagcgc cggtcaagac cggctccacg agcgcgccgc
      661 tgttgttcac gaacgcgacc ggctcgacct gcgcgtccgc gccctccgct ttcgacacga
      721 tgaagatgac ggcctctcgc gacggcgtcg tgcgccgctg agcgcgcgcg gcgacatgcg
      781 cgggcggcgc cgcgtgcatg agaaggagca gcgccgcgac gaacgcggtg gccctgtggg
      841 attgggggtt catcgatttc gcgtcccggt cgcggcggcc gacattacaa cgggccgcgc
      901 ttgttctttc tctcagatgg cgtcgcgctt ctccttgtcg ttgtagccgg ggtgaacttt
      961 ctcggagacg accttcgcct gcatgaagag gccgaggtag tcgcgcccgc cggccttcga
     1021 gtcggtgccg gacatgttga agccgccgaa ggggtggacg ccgacgagcg cgcccgtgca
     1081 cttgcggttg aggtagaggt tgccgacgtg gaactcgcgg cgcgcgcgtt cgagcttctc
     1141 gcggtcgttc gtgtagacgg cgccggtcag gccgtactcg gtgtcgttgg cgatgcggag
     1201 cgcctcgtcg aagtccgccg ccctgatgca ggcgagcacg gggccgaaaa tctcctcctg
     1261 ctcgatgcgg tcgccgggct tcacgtcggc gatgacggtc ggctcgatga agaagccctg
     1321 ctcgccgtcg ctgccgccgc ccgcgatgag gcgcccgccg tccgcctgcc ccttctcgat
     1381 gtagtcgctg atgctcttaa acgccttgtt gttgatgacg gcgctcatcg tcgtcgaggg
     1441 gtctaagggg tcgccgacct tgagacgcgc cgtgcgctcg gcgatgagtt cgagcatcgg
     1501 ctcgtagacg gcttcgtgga tgatcgcgcg cgagcaggcc gagcacttct gcccctgaaa
     1561 gccgaaggcc gactgcacga cgccggtcgc cgcctcgtcg aggtccgtgt ccgcgtccac
     1621 gatgatggcg tccttgccgc ccatctccgc gacgacgcgc ttgatccaga tttgcccgtc
     1681 gttcaccttc gccgcgcgct cgttgatgcg caggcccacc tccttcgagc ctgtgaacgc
     1741 gacgtagcgc gtcttcggat gatcgaccac gacatcgccg acctccgcgc ccgaaccggt
     1801 catgaagttg acgacgccgg gcggcatccc cgcctcttcg agcagctcga agaacttgta
     1861 ggcgatggcc ggcgcgtccg aagagggctt gaggacgacc gtgttgcccg tgacgaccga
     1921 ggccacggtc atgcccgcca tgatggcgag cgggaagttc cacggcggga tgacgacgcc
     1981 gacgcccaag gggatgtacg tcagccggtt ctcctcgccc gcgatcttca cgagcggctg
     2041 cggcgcggcg tagcgcagca tctcgcgcgc gtagaagtcg cagaagtcaa tcgcctccgc
     2101 cgtgtcgccg tccgcctccg gccacgtctt cgccacctcg tgcaccatcc acgcggagaa
     2161 gtagtggcgc cgctcgcgca agagcgatgc gacgcggaag agcaggtctg cgcgctgctg
     2221 cggcagcgtg cggctccacg tctgaaaggc ttcgctggcc ttctcgaccg cggcgcgcgc
     2281 cagctccggc gtcgccttgt ggaagcgccc gaccacttcg gtcttcttcg ccgggttca
//
LOCUS       DBNGOH010000940         3935 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1425133, whole genome shotgun sequence.
ACCESSION   DBNGOH010000940 DBNGOH010000000
VERSION     DBNGOH010000940.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3935)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3935)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3935
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1425133"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..792
                     /locus_tag="AC159P_18340"
     CDS             <1..792
                     /locus_tag="AC159P_18340"
                     /inference="COORDINATES: protein motif:HMM:NF044287.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nSTAND1 domain-containing NTPase"
                     /protein_id="HIR3697458.1"
                     /translation="PVALGGGDISPRLVNQLLNDIGDNPDQLPVLQHLLMRVWDEWKE
                     RRLEIVSEEGGVTVRRPHREAHEGEAIDLCCYEAVGGMARALSHHADEAFNELPDERH
                     REVAEKVFKALTEKGTDNREIRRQITLGELCAETAATLAETRAVVEAFRRPGRSFLMP
                     PAGVELNAESLIDISHESLIRNWERLSKWVDEEAQSARAYRRLAETAILYREGKEPPL
                     QDPALQLALDWRDKTKPHKAWADHYHPGYEAVVTYLDDSVAAREK"
     gene            937..1680
                     /locus_tag="AC159P_18345"
     CDS             937..1680
                     /locus_tag="AC159P_18345"
                     /inference="COORDINATES: protein motif:HMM:NF024298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HIR3697459.1"
                     /translation="MKQVERQRTDAVVIAAKARTAEEDALKQKGLAEQSEKDARTAQD
                     EALEQKRIADEAKALAEEEARLANASEARATDALSLAEKARQRAIAAERLSNQLLYAA
                     DSNLAQHLTDKGRFARARGVLDELRPAPGEDDLRGFEWYYLHRLSGVEGAQLEGHQKT
                     VNTVAISPTGGVLATGSYDGTVKLWAVSTGREVATLRGHTREIFSVAFGRNEQGSFLA
                     TEGWDGKIGLWYAPQDEEVAAPRRRPEEK"
     gene            1760..2260
                     /locus_tag="AC159P_18350"
     CDS             1760..2260
                     /locus_tag="AC159P_18350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HIR3697460.1"
                     /translation="MGLSEALLPEFDHEMATTRKTLERVPGEKFDWKPHEKSTTLGAL
                     ATHLANLPSWVGHTIDKDELDQPPLRIPPAHTRDEVLAAFDRNVAAARAALAGASDER
                     LLGPWTLLQGGNKVLTLPRAAVLRSFVLSHSIHHRAQLGVYLRLNDIPVPAVYGPSAD
                     ESPFQQ"
     gene            2494..3000
                     /locus_tag="AC159P_18355"
                     /pseudo
     CDS             2494..3000
                     /locus_tag="AC159P_18355"
                     /inference="COORDINATES: protein motif:HMM:NF009544.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
     gene            complement(3112..3816)
                     /locus_tag="AC159P_18360"
                     /pseudo
     CDS             complement(3112..3816)
                     /locus_tag="AC159P_18360"
                     /inference="COORDINATES: protein motif:HMM:NF009544.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
BASE COUNT          731 a         1287 c         1316 g          601 t
ORIGIN      
        1 cctgtggctt tgggcggcgg cgacatctcg ccccggctcg tcaaccaact gctcaacgac
       61 atcggcgaca atccggatca actgcccgtc ttgcagcacc tgctgatgcg cgtctgggac
      121 gagtggaagg agcggcggct ggagatcgtc agcgaagaag gaggagtgac cgtccgcagg
      181 ccgcacaggg aggcgcacga gggcgaagcg atcgacttat gttgctacga ggccgtcggc
      241 gggatggccc gcgcgctctc gcatcacgcg gacgaagcct tcaacgaatt gcccgacgag
      301 cgccaccgcg aggtcgccga gaaggtcttc aaagccctga ccgagaaggg gacggacaac
      361 cgcgagatac gccgccagat cacgctcggc gagctgtgcg cggagaccgc cgcgaccctt
      421 gccgagacgc gcgccgtcgt cgaagccttc cgccggccgg gccgctcgtt cctgatgccg
      481 ccggcgggcg tcgagctgaa cgccgagtcg ctgatcgaca tttcgcacga gagcctgatc
      541 cgcaactggg agcggctgag caagtgggtg gacgaggagg cgcagtcggc gcgcgcctac
      601 cggcgcctcg ccgagaccgc gattctttac cgcgagggca aagagccgcc gctccaggac
      661 ccggcgctgc aactggcgct cgactggcgc gacaagacca agccgcacaa ggcctgggcc
      721 gaccactatc accccggcta cgaggcggtc gtcacctacc tcgacgacag cgtcgccgcg
      781 cgcgaaaagt agcgccgcag ggaggcgagc taccgtcgct cccgctggct gacgctcctc
      841 ctgctgctcg ccttcgtccc gaccttcgcc gcgctcgcct acgctttcca gcaagggagt
      901 aaggctcgga gggccaacgc cgagttacag gaggtcttga aacaagtcga gcgacagcga
      961 accgacgcgg tagtcatcgc ggcgaaagca cggactgccg aagaagacgc cctcaagcag
     1021 aagggcctcg ccgaacaaag cgagaaggac gcgcggacgg cgcaagacga agctctggag
     1081 cagaagagaa tagccgacga ggccaaggcg ctggccgaag aggaggcgcg gctggcaaac
     1141 gcgagcgaag cgcgggccac cgatgccctg agtctcgcgg aaaaagcgag gcagagggcc
     1201 atcgccgccg agaggttgag caaccaactg ctctacgcgg cggacagcaa tctcgcacag
     1261 catcttaccg ataaaggccg attcgcgcgg gcgcgcggcg tgctcgacga gttgcggccc
     1321 gcgccggggg aggatgacct gcgcggcttc gagtggtact acctccacag gctctccggc
     1381 gtcgagggcg cgcagctcga aggacatcaa aaaaccgtca acaccgtcgc catctcaccg
     1441 acaggcgggg tgctcgccac cggcagctac gacggcacgg tgaagctgtg ggccgtgagc
     1501 accgggagag aagtggccac gctcagaggg catacccgcg aaatcttctc cgtcgccttt
     1561 ggaaggaacg agcagggcag cttcctggcg accgaaggct gggacgggaa gatagggctt
     1621 tggtacgcgc cgcaggatga agaggtggcc gcgccgcgaa gacgaccgga agagaaatga
     1681 cgacgcacta aaagcttggc gggcggacgc gcgggccgct cgtcgtcgtt ggtttatcga
     1741 cacacgcgga ggtgaagaga tgggtctcag tgaagcgttg ctgccggagt tcgaccacga
     1801 gatggcgacc acgcgcaaga cgttggagcg tgtccccgga gagaagttcg actggaagcc
     1861 gcacgagaag tccaccacgc tcggcgccct cgccacgcat ctcgccaacc tgccgagctg
     1921 ggtcggccac accatcgaca aggacgagct ggaccagccc ccgctgcgca tcccgcccgc
     1981 gcacacgcgc gacgaagtgc tcgcggcctt cgaccggaac gtcgcggcgg cgcgcgcggc
     2041 cctcgcgggc gcgagcgacg agcggctgct cggcccgtgg acgctgctgc aaggcggcaa
     2101 caaggtcttg accttgccgc gcgccgccgt cctgcgcagc ttcgtcttga gccacagcat
     2161 ccaccaccgc gcgcagctcg gcgtctacct ccgcctcaac gacatccccg tccctgcggt
     2221 ctacggcccg tcggcggacg agtcgccgtt ccaacagtag gcggtaggca gtaggcggta
     2281 ggcagtaaaa ggcaagaagg gcgaagaggc ttgagaggta aaatcccgag cctcgatctc
     2341 ggattacttg acgaacgaag aggcttgaga cgttagctct cacgcctctt gctctttact
     2401 gcctaccgcc tacggcctac tgcctactgc ttttcgcccc cgcggtgtta gaatcgacgc
     2461 acagtcgaac aaccaacgga gcgtgaaatt tttttgagcc gcgaactgag agttctcaac
     2521 aaccctgcct cgacgaggcc gccgcgcggg ccgaagcccg agtggctgaa gattcgcatc
     2581 ggcgacccga ccaaccagaa cgccgtcctc aagctgatcg agggcttgaa cctgcacacc
     2641 gtctgccagg aggcgcgctg ccccaacatc ttcgagtgct ggagcgaccg cacggcgacc
     2701 ttcatgatcg gcggcgacat ctgcacgcgc cactgcggct tctgcgccgt cggcaagggc
     2761 gcgcccggca cgctcgacgc cgaagagccg cgccacgtcg ccgaggcggt caaacatctc
     2821 gacctcgcgc acgccgtcat cacctcggtc aaccgcgacg acctcccgga cggcggcgcc
     2881 gcccactggg ccgagaccat ccgcgaagtg cgcgccatga accccggctg ccgcgtcgaa
     2941 gttttgattc ctgattttaa cggcgacgag cgggcgctcg atgccgtgct cgacgccgag
     3001 ccggacgtgc tcaaccacaa caccgagacc atcgcgcgtc tctacaagcg cgtgcggctg
     3061 gaggtactgg ccgatggtca tgatgtcgca cgagaccgag cgcaggtctt tcatcagctc
     3121 gaccacctct tcgaacgtct cgccgaggcc gaccatgatg ccgctcttcg tcaacatccc
     3181 gcgcgcctcc cggtcgcggc gcgcggcggc gcgactcagg agcgtcaagg tctgctggta
     3241 gttcgcgtcc ggccggacgc gccggtacag gcgcgcgatg gtctcggtgt tgtggttgag
     3301 cacgtccggc gcggcgtcga gcacggcgtc gagggcccgt tcgtcgccgt tgaagtcggg
     3361 gatcaggacc tcgacgcggc agtcggggtt gtgcgcgcgc acctggcgga tggtctcggc
     3421 ccagtgggcg gcgccgccgt cggcgaggtc gtcgcggttg accgaggtga tgacggcggg
     3481 cgcgaggtcg agggtcttca ccgcctcggc gacgtggcgc ggctcgtccg ggtcgagcgg
     3541 gatgcccgcg cccttcgaga cggcgcagaa gccgcagtgg cgcgtgcaga tgtcgccgcc
     3601 gatcatgaag gtcgccgtcc tgtccgtcca gcactcgaag atgttcgggc agcgcgcctc
     3661 ctggcagacc gtgtggaggt tcaggttctc gatcagtttc aggacgtagt tctggttgga
     3721 agggtcgccg aggcgtacct tcagccactc gggcttgggc cctctcgttc gcggggtcgc
     3781 gggggggttg ttaatgacgc gcagctcgcg gctcaaactc tttctccttg ttcgactgtt
     3841 cggctatgca atttgaatgt tctgttattc aactgaaccg attttaacac ccggttgtag
     3901 ctttgtcctg cggcgcgcgc cgccaaaaat tatgt
//
LOCUS       DBNGOH010000941         2690 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4356887, whole genome shotgun sequence.
ACCESSION   DBNGOH010000941 DBNGOH010000000
VERSION     DBNGOH010000941.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2690)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2690)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2690
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4356887"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(9..341)
                     /locus_tag="AC159P_18365"
     CDS             complement(9..341)
                     /locus_tag="AC159P_18365"
                     /inference="COORDINATES: protein motif:HMM:NF013260.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR3697461.1"
                     /translation="MRGAVELIAALRDESAPRPAPVRIDPADLLAGEGRYDVDFREVR
                     GQLAAKRALEVASAGSHNILLIGSEHLAHKPRPACPGALHRAAGSLSRSRPPSPQVLR
                     APAAFPRK"
     gene            complement(354..839)
                     /locus_tag="AC159P_18370"
     CDS             complement(354..839)
                     /locus_tag="AC159P_18370"
                     /inference="COORDINATES: protein motif:HMM:NF024931.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HIR3697462.1"
                     /translation="MLFRTQSAAVYGIDADLVDIEVDLTPARGESDSPSSVTIVGLPD
                     LAVRESRERIRAAINNSAFFFPFHKTTINLAPADVRKEGASFDLPIALGILGANGDLG
                     DAEGLTDTLSVGELSLDGRVRPVRGALPIAVRARDAGLRNLVMPEENAREAAVVAGVR
                     V"
     gene            complement(1047..>2690)
                     /locus_tag="AC159P_18375"
     CDS             complement(1047..>2690)
                     /locus_tag="AC159P_18375"
                     /inference="COORDINATES: protein motif:HMM:NF013618.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008933 - peptidoglycan lytic
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HIR3697463.1"
                     /translation="LRDVTNNAERDDPRVQSIIERAENHYKLGELNLKDGKRQPAREE
                     FDKAVDSVLESGMDVRSNPKLQRYYLELVERVYRMEVPQGVPSSPQQQGATFVEVAQT
                     GQQTGTPAPPQVGFVEQKFEPSNLDELSKLVLTKDEEKISDEQVAEAELIKNTLDFQF
                     KLHPLVVQYINYYQGRGRATMETGLRRSGQYMTMARRIFREEGVPEDIVWLGQVESSW
                     RPTARSWAAASGLWQFIPGTGSRFGLRQTAYIDERNSFEKATRASAQYLKWLARRYNG
                     NWELAMGAYNTGEGNVDRAIGRAGSADFWAIYPYIAQETRNYVPNILAVILIAKNPEK
                     YGFRNVQRMPPLAYDVVSVPSSTSLHLIANLTDSNVDYLRSINPELRRDMTPPGEQYH
                     VRVPPGRGRAFVDLLKRVPAERRNQLARVIQVSPGESFEAVAARTGTSIAQLQQWNGG
                     VDLKGGGKIVVPGGSVVKTAKTYERAGVASAPVGNSLRNYTAKGGETLAQVAASHGAS
                     VEEVAKINGLPADAKLERGRLIKVPTSSAAPAQQTRRRR"
BASE COUNT          383 a          876 c          968 g          463 t
ORIGIN      
        1 ggcggccttc actttcgagg aaaggcggct ggcgctctca gcacttgggg cgaaggtggt
       61 cgggaacggg aaagactacc tgcggctcga tgtaacgctc ccggacacgc cggtcggggt
      121 ttatgagcca ggtgttctga cccaatcagc aggatgttgt gcgagccggc ggaggcgact
      181 tcgagcgcgc gcttggcggc gagctgtccg cgcacctctc ggaagtcaac gtcgtagcgg
      241 ccctcgcccg cgagcaggtc ggcggggtcg atgcggacgg gcgcgggccg cggcgcggac
      301 tcgtcgcgca gcgccgcgat cagctcgacc gcgccgcgca aggaagagac cggctaaacg
      361 cggacgcccg cgaccaccgc cgcttcccgt gcgttctctt cgggcatgac gaggttgcgc
      421 agccccgcgt cgcgcgcgcg caccgcgatg ggcagcgcgc cgcgcacggg ccgcacgcgc
      481 ccgtcgagcg agagttcgcc gaccgagagc gtgtcggtca gcccttcggc gtcgccgagg
      541 tcgccgttcg cgccgaggat gccgagcgcg atgggcaggt cgaagctcgc gccctctttg
      601 cgcacgtccg ccggcgcgag gttgatcgtc gtcttgtgga aggggaagaa gaaggcgctg
      661 ttgttgatgg cggcgcggat gcgctcgcgc gactcgcgca cggccagatc gggcaggccg
      721 acgatggtga cggaagaggg cgaatcgctt tcgccgcgcg cgggcgtgag atcgacttcg
      781 atatcgacga ggtcggcgtc gatgccgtag acggcggcgg actgtgttct gaagagcatg
      841 cccgtgaggc gctctcagca ggggcggagc gcgacgcatt cagcgcgagt gaggggcggg
      901 cggcttaacc actcactcga cggttgctct ctcgggccag gtgtgaaaac gacggagcgc
      961 gctgtcggag gtcgatccga agcgcgctcg tccgtgctct tttcggcgtg cgacgccgct
     1021 cggaagcggg gccgcgtctg ctgtccttac cggcgacggc gcgtctgctg tgcgggcgca
     1081 gccgaagagg taggaacctt gatgaggcga ccgcgttcga gcttcgcgtc cgccggcagg
     1141 ccgttgatct tcgccacctc ttcgaccgag gcgccgtggc tggccgcgac ctgcgcgagc
     1201 gtttcgccgc ccttcgccgt gtagttgcgc agactgttgc cgaccggggc cgaggcgacg
     1261 cccgcccgct cgtaggtctt cgccgtcttc acgacgctgc cgccggggac gacgatcttg
     1321 ccgccgccct tcagatcgac gccgccgttc cactgctgca actgcgcgat gctggtgccg
     1381 gtgcgcgcgg ccacggcctc gaacgactcg cccggcgaga cctggatgac gcgcgcgagc
     1441 tggttgcggc gctcggcggg gacgcgcttc aggagatcga cgaacgccct gccgcggccc
     1501 ggaggcaccc gcacgtgata ctgctcgccc ggcggcgtca tgtcgcggcg caactccgga
     1561 ttgatgctgc gcaggtaatc gacgttcgag tccgtcaagt ttgcgatcag gtgcagactc
     1621 gtcgaagagg gcacgctcac cacgtcgtac gcgagcggcg gcatccgctg tacgttgcgg
     1681 aagccgtact tctcggggtt cttcgcaatc aggatgacgg cgaggatgtt cggcacgtag
     1741 ttgcgcgtct cctgcgcgat gtacgggtag atggcccaga agtcggcgga gccggcgcgc
     1801 ccgatggcgc ggtcaacgtt gccctcgccc gtgttgtagg cgcccatcgc cagttcccag
     1861 ttgccgttgt agcggcgggc gagccacttc aggtactgcg ccgaggcgcg cgtcgccttc
     1921 tcgaagctgt tgcgctcgtc gatgtaggcg gtctgcctga gaccgaagcg cgagccggtg
     1981 ccggggatga actgccagag gccggaggcc gcggcccacg agcgcgcggt cgggcgccag
     2041 gagctttcga cctgcccgag ccagacgatg tcctcgggca cgccctcctc gcggaaaatc
     2101 ctgcgcgcca tcgtcatgta ctggcccgag cggcgcaggc ccgtctccat cgtggcgcga
     2161 ccgcggccct gatagtagtt gatgtactgg acgacgagcg ggtggagctt gaactggaag
     2221 tcgagcgtgt tcttgatcag ctcggcctcg gcgacctgct cgtccgaaat cttctcctcg
     2281 tccttcgtca gcacgagctt cgacagctcg tcgagattcg acggctcgaa cttctgctcg
     2341 acgaagccga cctgcggcgg cgcgggcgtg ccggtctgct ggccggtctg cgcgacctcg
     2401 acgaacgtgg cgccctgctg ctgcggtgac gagggcacgc cctgcgggac ttccatccgg
     2461 tagacgcgct cgaccagttc caaatagtag cgctggagct tggggttcga gcgcacgtcc
     2521 atgccggatt cgagcaccga gtccacggcc ttgtcgaact cctcgcgcgc gggctggcgc
     2581 ttgccgtcct tgaggttcag ctccccgagc ttgtagtggt tctcggcccg ctcgatgatg
     2641 ctctgcacgc gcgggtcgtc gcgctcggcg ttgttggtca cgtcacggag
//
LOCUS       DBNGOH010000942         1696 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4357132, whole genome shotgun sequence.
ACCESSION   DBNGOH010000942 DBNGOH010000000
VERSION     DBNGOH010000942.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1696)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1696)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1696
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4357132"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            291..>1696
                     /locus_tag="AC159P_18380"
     CDS             291..>1696
                     /locus_tag="AC159P_18380"
                     /inference="COORDINATES: protein motif:HMM:NF012311.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HIR3697464.1"
                     /translation="MLRRITASLISLSLLAVSLAPIATLAAQNKSHDVEPQQRKLAPE
                     FDSAAASSSTSGDTVRVIIQTKGRPGAAQDEAISRRGGRKLASFDALDAVVASVPAGS
                     LAELAAREDVTYISPDRRVSAQLGVTRETTGASIAQAGQAQTQGVTGKGIGIAVIDSG
                     ISASHPDFRNNDNSRVVAAVDFTGESGAVQGDRGVVLGDKGVVLGDKGVVLGDKGVVL
                     GDQGVVLGDGVVLGDKDGHGTGVASVAAGNGAGSAGYAGSYVGIAPEAHLIDLKVLGS
                     TGTGTTSAVMAAVNWAIVNQRRHNIRVLNMSLGAPVVESYKSDPLCQAVARAVRSGLV
                     VVASAGNRGRTETIIGHNTDGTPIYQPVYGSINSPGNSPYVITVGATDARGTANRSDD
                     TMAQFSSKGPTQVDHLAKPDLVAPGRAIVAAMSQEDNPNMAQQRPDRVVAAPAGARAN
                     MYYSYYGTSFSAPVVSGTV"
BASE COUNT          297 a          566 c          569 g          264 t
ORIGIN      
        1 gagggtgagc tgtaacttgt tgcaggtgaa tgggtaagct ttctttttgg ggtgtgttcc
       61 gtggtccctt gtttgtggca cgttcgttga agtagagagg ggcagatcgt tgaatgcggc
      121 ttccgggttg agagtcatgc ggccttgaac tctttatctt gctggcctct cacggccaga
      181 tttgaagtcg cttcaacgat ccctaatccg gcagctttga attgacgctt ccggttcccc
      241 cgacgcgatg ggcagcgcag aacgggaagc acagaaagaa gggacacgaa atgttacgtc
      301 gtatcacagc cagcttgatc agcctcagcc tcttagctgt ttctcttgcc cccattgcga
      361 ccctcgccgc acaaaacaag agccacgacg tagaaccgca acagcgcaag ctggcgccgg
      421 aattcgattc cgccgccgct tcgtcgtcca cctccggcga caccgtgcgc gtcatcatcc
      481 agaccaaggg ccggcccggc gccgctcagg acgaggcgat ctcgcgccgc ggcggcagga
      541 agctggccag cttcgacgcg ctcgacgcgg tcgtcgccag cgtcccggca ggctccctcg
      601 cggaactggc cgcgcgcgaa gacgtgacgt acatctcgcc cgaccgcagg gtctccgcgc
      661 agttgggcgt gacgcgcgag acgaccggcg cgagcatcgc gcaggcgggt caggcgcaga
      721 cgcagggcgt gacgggcaag ggcatcggga tcgccgtcat cgacagcggc atcagcgcct
      781 cgcaccccga cttcaggaac aatgacaatt cgcgcgtcgt cgccgccgtt gacttcacgg
      841 gcgagagcgg cgcggtgcag ggcgacaggg gcgtcgtgct cggcgacaag ggcgttgtcc
      901 tcggcgataa gggtgtggtg ctgggcgaca aaggcgtcgt gctgggcgat cagggcgtcg
      961 tgctcggcga cggcgtggtg ctcggcgaca aagacgggca cggcacgggc gtggcgagcg
     1021 tggcggcggg taacggcgcc ggttcggcgg gttatgcggg cagctacgtc ggcatcgcgc
     1081 ccgaggccca cctgatcgac ctgaaggtgc tgggcagcac cggcacgggc acgacgagcg
     1141 ccgtgatggc cgccgtcaac tgggccatcg tcaaccagcg gcggcacaac atccgcgtcc
     1201 tcaacatgag cctcggcgcg ccggtggtcg agtcttacaa gtccgacccg ctgtgccagg
     1261 cggtggcgcg cgccgtccgc agcggcctcg tcgtggtggc gagcgccggc aaccgcgggc
     1321 gcaccgaaac catcatcggc cacaacacgg acggcacccc catctaccag ccggtctacg
     1381 gcagcatcaa cagccccggc aacagcccct acgtcattac ggtcggcgcg accgacgcgc
     1441 gcggcacggc caaccgctcc gacgacacga tggcgcagtt ctcctcgaaa ggcccgacgc
     1501 aggttgacca cctcgccaag cccgacctcg tcgcccctgg ccgcgcgatc gtcgccgcga
     1561 tgtcgcagga ggataacccg aacatggcgc agcagcgccc cgaccgcgtc gtcgcggccc
     1621 cggcgggcgc cagggcgaac atgtactaca gctactacgg cacgtccttc tccgcgccgg
     1681 tcgtcagcgg cacggt
//
LOCUS       DBNGOH010000943         2775 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4540630, whole genome shotgun sequence.
ACCESSION   DBNGOH010000943 DBNGOH010000000
VERSION     DBNGOH010000943.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2775)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2775)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2775
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4540630"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1152)
                     /locus_tag="AC159P_18385"
     CDS             complement(<1..1152)
                     /locus_tag="AC159P_18385"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3697465.1"
                     /translation="MGFDKAKAIRAAEKSLAQGKIPAAIQEYRRIVENDPEDYSALNT
                     LGDLHARVDQKPDAVACYRRVAEHYRDQGFALKAIAMFKKVTRFAPGEHATALTLARL
                     YEQQGLVVDARAQYLVAADALARAGDTREALEVLRRIADLDPANTNIRLRLAESYVAE
                     DMTDDAAEVYTEAGERLLARAEYERALDCYKRALSYRPFSHAALQGLVAVHAALGTAD
                     EAAQALEEAVKSRPGDTELRAMLARSYIDSEEAEKAERIIEGIVRQEPANYSLFFDVA
                     RLYLRQGEADAAARVLGHIIESALAGRHEEQFLELLQEVLARDPEQMEALHLLVRVHT
                     WQRDDLRLRKALERLADAAETTGDEDEERRALAQLARLSPEEPRYRERLQ"
     gene            1438..>2775
                     /locus_tag="AC159P_18390"
     CDS             1438..>2775
                     /locus_tag="AC159P_18390"
                     /inference="COORDINATES: protein motif:HMM:NF016800.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HIR3697466.1"
                     /translation="MNTPTDENRRGLRATRAAEGVALAGLLVYAMAAPHSIAGSWMGL
                     SVAVLGWLARTLLTRRAGVRRSLMDAPLWLLFAWTVLSSALSYEPQESLPKLVNAATF
                     LVFYVAQSLLTRRRVVMLALVMIASGVAGVLWSLGELAVGRGVVVTEVRAESPFRATL
                     LQAGDAVWRVNGRRVSSIAEVDERVRAAKAGEPLRVSVISRGEHVEWQAPPVTEESKT
                     APSPSGLAGGGPTRSFRASGWTRHYETHAECLQMIAQLALGLALALWLRRGPRAHALL
                     AAAAFALLALGVALTAMRTVLVAFAVGALVVAWRAGIGRRARLLVAAAVVVVLALGAL
                     TVWRTRAGGALRLGDASSSTRLRVATVAASRVPLHPVFGHGMDAAHRHWREWGFPGDD
                     MLHAHSTPVQLAFERGLPALLFWLWLMLAFWRMCARAERLWRGAGDDAAHGLAL"
BASE COUNT          339 a          929 c         1040 g          467 t
ORIGIN      
        1 ctgcaagcgc tcgcggtagc gcggctcttc gggcgagagg cgggcgagct gcgcgagcgc
       61 gcggcgctcc tcgtcttcgt cgccggtcgt ctccgccgcg tcggcgagcc gttcgagcgc
      121 cttgcgcaga cgcaggtcgt cgcgctgcca ggtgtgcacg cgcacgagca ggtggagcgc
      181 ctccatctgc tcggggtcgc gcgcgaggac ttcctgaagg agttcgagga actgctcctc
      241 gtgccgaccg gcgagcgccg actcgatgat gtggccaagc acgcgcgcgg cggcgtccgc
      301 ctcgccctgc cgcaggtaga gccgcgccac gtcgaagaag agcgagtagt tcgcaggctc
      361 ctgccggacg atgccctcga tgatgcgctc ggccttctcg gcctcctcgg aatcgatgta
      421 ggagcgagcc agcatcgcgc gcagctccgt gtcgccgggc cggctcttga ccgcctcttc
      481 gagcgcctgg gccgcctcgt ccgccgtccc gagcgcggcg tggacggcga ccagaccttg
      541 cagcgccgcg tgcgagaagg gccggtacga gagcgcgcgc ttgtagcagt cgagcgcccg
      601 ctcgtactcg gcgcgcgcca gcagccgctc gcccgcctcg gtgtagacct cggcggcgtc
      661 gtccgtcatg tcttcggcga cgtagctttc ggcgaggcgc aggcggatgt tggtgttcgc
      721 ggggtcgagg tcggcgatgc ggcgcagcac ttcgagcgcc tcgcgcgtgt cgcccgcgcg
      781 ggccagcgcg tccgccgcga cgaggtactg cgcacgcgcg tccaccacca gtccctgctg
      841 ctcgtagagc cgcgcgagcg tgagcgccgt cgcgtgctcg ccgggggcga agcgcgtgac
      901 cttcttgaac atcgcgatgg ctttgagcgc gaagccctga tcgcggtagt gttcggcgac
      961 gcggcggtag caggcgacgg cgtcaggttt ctgatcgacg cgcgcgtgca ggtcgccgag
     1021 cgtgttgagc gcggagtagt cttcggggtc gttctcgacg atgcgccggt attcctggat
     1081 ggccgccgga atcttgccct gggcgaggga cttctcagcc gcacggatgg cttttgcttt
     1141 gtcgaatccc atcgaaaaat ttagccgtcg agaagccgac agagaaggaa ccgacgcgag
     1201 gcgtgtcggg ctgaagatta cggcgcccgg cgggtacatc ttgtcatggc ttaaccctta
     1261 gtcttccgag gaagggcagt tttgtacact aacacccgct cgcgacgagt gtcaacaagc
     1321 tcctccggcg gcgtttgcgc gcgctaaccg ctgataaaaa cggtgttcac gcccggcccg
     1381 cgtgcgttat aacatcacgc gcgaggcacg gagctacgcc cgcatcaagc tgcaaacatg
     1441 aacacaccga ccgacgaaaa caggcgcggc ctccgtgcga ctcgcgcggc tgagggcgtg
     1501 gcgctcgcgg ggctgttggt ctacgcgatg gccgcgccgc attcgattgc ggggtcgtgg
     1561 atggggctgt cggtggccgt gctcgggtgg ctcgcgcgga ctttgttgac gcggcgggcg
     1621 ggcgtccggc ggagcttgat ggacgcgccg ctgtggctgc tcttcgcgtg gacggtgctc
     1681 tcgtccgcgc tctcgtacga gccgcaggag agtctgccca agctcgtcaa cgcggcgacc
     1741 tttctcgtct tctacgtcgc gcaaagtttg ctgacgcggc gaagggtggt catgctcgcg
     1801 ctcgtcatga tcgcgtcggg cgtggcgggc gtgttgtgga gcctgggcga gctggcggtc
     1861 gggcgcggcg tcgtcgtgac ggaggtgcgc gccgaaagcc ctttccgcgc gacgctgctt
     1921 caggcgggcg acgcggtgtg gcgcgtcaac gggcggcgcg tctcttccat cgcggaggtt
     1981 gacgagaggg tgcgcgcggc gaaggcgggc gagcctttgc gcgtgagcgt catctcgcgg
     2041 ggcgagcacg tcgagtggca ggcgccgccc gtgacggaag agtccaagac ggcgccgtct
     2101 ccgtcgggac tcgcgggcgg cgggccgacg cgcagcttcc gcgcttcggg gtggacgcgc
     2161 cactacgaga cgcacgccga atgtttgcag atgatcgcgc agctcgcgct cggcctcgcg
     2221 ctcgcgctct ggctgaggcg cgggccgcgc gctcacgcgc tgctcgcggc ggcggccttc
     2281 gcgctgctgg cgctcggcgt tgcgctgacg gcgatgcgga cggtgctcgt cgccttcgcc
     2341 gtcggcgcgc tcgtggtcgc gtggcgcgcg ggcatcggtc ggcgcgcgcg gctcctcgtc
     2401 gcggcggcgg tggtcgtcgt cctcgcgctc ggcgcgctga cggtgtggcg cacgcgcgcc
     2461 gggggcgcgc tccgcttggg cgacgcgagc agcagcacgc ggctgcgcgt ggcgacggtc
     2521 gcggcctcgc gcgtgccgct gcacccggtc ttcggccacg gcatggacgc ggcgcaccgc
     2581 cactggcgcg agtggggttt ccccggcgac gacatgctgc acgcgcactc gacgcccgtc
     2641 cagctcgcct tcgagcgcgg gctgcccgcc ctcctcttct ggctctggct gatgctcgcc
     2701 ttctggcgga tgtgcgcgcg cgccgagcgg ctctggcgcg gggccggaga cgacgccgcg
     2761 cacggcctcg ccctc
//
LOCUS       DBNGOH010000944         3139 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3992834, whole genome shotgun sequence.
ACCESSION   DBNGOH010000944 DBNGOH010000000
VERSION     DBNGOH010000944.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3139)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3139)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3139
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3992834"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..359
                     /locus_tag="AC159P_18395"
     CDS             <1..359
                     /locus_tag="AC159P_18395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697467.1"
                     /translation="GPGGADLLLPNDEDAGYWAEFQIGRTQQRGDTLFGYTFMRIEKD
                     AILSPFNASDISITTDVRVHRFIASYAADPRVVLSLTGFFTQRPNGLLGPFAATPPGS
                     LNRTLTRLQLDTIFRF"
     gene            complement(463..2520)
                     /locus_tag="AC159P_18400"
     CDS             complement(463..2520)
                     /locus_tag="AC159P_18400"
                     /inference="COORDINATES: protein motif:HMM:NF014606.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3697468.1"
                     /translation="MKALINNLAALAVVWCLIVPAAGATRPPQADKKSPPKTAPDDGA
                     RGGAVRGNNGLVRGREHEKAAEAAFELGVKYGRAGRYREAVEAFREAVLYKPDHGDAH
                     FSLGHALYDLGRWAEAAESFKRAIALDPKDTEAFNYLGAAYFRQGLYDRAVEAYKGAI
                     LVKPNLADVRYNIANAFYKLGRYEPAILYYKETSYIGRRSARLFNDMGVAYAEWGRYA
                     EATDAFKRATGQDSDDAYAHNNLALSYFFQGQHEEAAKAFERATRLAPRDEIIRRNAD
                     LAATGSGGGETAVGDRVSLLVKTGAGGGRREVRWLGRSDVIHGVAASDAPARAGDDTE
                     AAAAPTPTVKSAPAPTPAQVALKSSAAAPTPTPTPIPTPPPTPTPAASGPARVEASGP
                     DTPVGEAAAAAPARSEPAPARDETEPAGEPATASAATADTAAPPAAAAAPALTDVYRV
                     GVGDVLDIRLLDDSPSRSTLYTVLAGGLVEYTPSGNSFAAAGKTTDEIAAHVKSELRR
                     LAIRDDPRVVVSVREYASHTVIISGLVAEPGAKVLRREAVPLYVVVADAQPLPEAGRA
                     LVTSDAGKQSTEVALDDQASMSTLVRPGDVISVLGRRQQFYFIGGKVEAPGQKDFHAG
                     ITLTQAILAAGGSLSASKTALVTRQTADGLLSATTYNLKQLMAGVAPDPPLQPGDRIE
                     VVR"
     gene            complement(2702..>3139)
                     /locus_tag="AC159P_18405"
     CDS             complement(2702..>3139)
                     /locus_tag="AC159P_18405"
                     /inference="COORDINATES: protein motif:HMM:NF013431.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HIR3697469.1"
                     /translation="FRLPARGEVAVPARRRWELAESLPTGRLLDVEGAYDLRGGRSLA
                     GLSLDDIYTGVYADADGRARCAISDLERGTRTVVEFDPREFPHVVVYTPPAPRRAVCV
                     EPNTCPTDAFNLQARGVESNVVTLGAGESATFDISLYVESLRP"
BASE COUNT          425 a         1085 c         1104 g          525 t
ORIGIN      
        1 cggggccggg cggcgccgac ctgctcctgc cgaacgacga ggacgcgggc tactgggccg
       61 agttccagat cggcaggacg cagcagcgcg gcgacacgct cttcggctac accttcatgc
      121 gcatcgagaa ggacgccatc ctgtcgccct tcaacgccag cgacatcagc atcacgacgg
      181 acgtgcgcgt ccaccgcttc atcgcgtcct acgccgccga cccccgcgtc gtgctctcgc
      241 tcacgggctt cttcacgcag cgccccaacg gcctgctcgg ccccttcgcc gcgacgcccc
      301 ccggctcgct caaccgcacg ctcacgcgcc tgcaactcga cacgattttc agattctagg
      361 aagggcacag gggaaaaggc gaaaagggtg aagacagagc aagctgcttg tcttcaccct
      421 tttcgccttt tcccctttta accttttccc ccttcttgtc tttcagcgga cgacttcgat
      481 tcggtcgccg ggctggaggg gcgggtcggg cgcgaccccg gccatgagct gcttgaggtt
      541 gtaggtggtc gcggacagca ggccgtcggc tgtctggcgc gtcacgagcg cggttttact
      601 cgcgctgagc gagccgcccg ccgccaggat ggcctgcgtc agcgtgatgc ccgcgtgaaa
      661 atctttctgg cccggcgcct cgaccttgcc cccgatgaaa tagaactgct gccgtctgcc
      721 gagcacgctg atcacgtcgc cggggcggac gagcgtgctc atcgacgcct gatcgtcgag
      781 cgcgacttcg gtgctttgtt tgcccgcgtc gctggtgacg agcgcgcggc ccgcctccgg
      841 caggggctgc gcgtcggcga cgacgacgta gagcggcacg gcttcgcggc gcagcacctt
      901 ggcgcccggc tccgcgacca gaccgctgat gatgaccgtg tggctggcgt attcccggac
      961 gctcaccacg acgcgcgggt cgtcacggat ggcgaggcgc cgcaactcgg acttgacgtg
     1021 cgccgcgatc tcgtccgtcg tcttcccggc ggcggcgaac gaattccccg acggcgtgta
     1081 ctccacgagg ccgcccgcca gcaccgtgta gagtgttgag cggctcgggc tgtcgtcgag
     1141 cagccggatg tcgagtacgt cgccgacgcc gacgcggtac acgtccgtca gcgccggggc
     1201 cgcggcagca gcaggcggcg cggcggtgtc ggccgtggca gcagacgccg tcgcaggctc
     1261 gcccgccggc tccgtctcgt ccctggccgg cgccggttcg ctcctggccg gcgcggcggc
     1321 ggcggcctct ccgaccggcg tgtcggggcc ggacgcttcg acacgcgcgg ggccggacgc
     1381 cgccggcgtg ggcgtgggcg gtggagtcgg tatgggcgtc ggcgtcggcg tcggcgcggc
     1441 ggcggaactc ttcaacgcca cctgtgcagg cgtgggggcc ggggcgcttt taaccgttgg
     1501 ggtcggggcc gcggcggctt ccgtgtcgtc ccccgcgcgg gcgggcgcgt cggacgccgc
     1561 gacgccgtga atcacgtcgc tccggcccag ccagcgcact tcgcgcctgc cgccgcccgc
     1621 gcccgttttg acgaggagcg agacgcggtc gcccacagcc gtctcgcccc ctccggaacc
     1681 cgtagccgcg aggtcggcgt tgcgccggat gatttcgtcg cgcggggcga ggcgggtcgc
     1741 ccgctcgaac gctttggccg cctcctcgtg ttgcccctga aagaagtagg agagcgccag
     1801 gttgttgtgc gcgtaggcgt cgtcggagtc ctgccccgtc gcccgcttga aggcgtcggt
     1861 cgcctcggcg taccggcccc actcggcgta ggcgacgccc atgtcgttga acagacgggc
     1921 cgacctccgc ccgatgtagc tggtctcctt gtagtacagg atcgccggct cgtagcgccc
     1981 gagcttataa aaagcgttcg cgatgttgta gcgcacgtcg gccaggttgg gcttcacgag
     2041 gatggcccct ttgtaggctt cgaccgccct gtcgtaaagc ccctgccgga agtacgcggc
     2101 gccgaggtag ttgaaggcct cggtgtcctt cgggtcgagg gcgatggccc gcttgaacga
     2161 ctcggcggcc tcggcccaac gtcccaggtc gtagagcgcg tggccgaggc tgaagtgcgc
     2221 gtcgccgtgg tcgggcttgt agaggacggc ttcgcggaag gcttcgaccg cctcccgata
     2281 ccggcccgcg cgcccgtatt tgacccccag ctcgaaggcc gcctcagccg ccttctcgtg
     2341 ttcgcgcccg cggacaaggc cgttattccc gcgcacggcg ccgccgcgcg cgccatcgtc
     2401 cggcgccgtc ttcggcgggg acttcttatc ggcctgcggc gggcgggtcg cgcctgcggc
     2461 gggaacgatc aggcaccaga cgacggcgag ggcggcgagg ttattgatca aagctttcat
     2521 catggtcctg ccgatgtgcg ggaaaggggc gtacgcttgg cggctgggga cggcgcggtc
     2581 gtacgtcgag acacttctta tcgggagaga agggtgtttg aagaagcgag tgtaaagcgt
     2641 cgcggcggcg gttgtctatc ccaaaccgta ctccttactc ctgcccgcga ccggacgcgc
     2701 gtcagggccg caggctctcg acgtagaggc tgatgtcgaa cgtcgccgac tcgcccgcgc
     2761 cgagcgtgac gacgttgctt tcgacgccgc gcgcttgcag gttgaaggcg tcggtggggc
     2821 acgtgttcgg ctcgacgcag acggcgcggc gcggggcggg cggcgtgtag acgacgacgt
     2881 gcgggaactc ccttgggtcg aactcgacga cggtgcgcgt gccgcgttcg aggtcgctta
     2941 tcgcgcaccg cgcgcgcccg tccgcgtccg cgtagacgcc cgtgtagatg tcgtcgaggc
     3001 tcaagcctgc gagactgcgc ccgccgcgca aatcgtaagc gccttcgaca tcgagcagcc
     3061 tgcctgtcgg cagactctcc gccaactccc aacgcctgcg cgcgggcacc gcgacctcgc
     3121 cgcgcgcggg gaggcgaaa
//
LOCUS       DBNGOH010000945         6819 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_509857, whole genome shotgun sequence.
ACCESSION   DBNGOH010000945 DBNGOH010000000
VERSION     DBNGOH010000945.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6819)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6819)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6819
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_509857"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..282)
                     /locus_tag="AC159P_18410"
     CDS             complement(<1..282)
                     /locus_tag="AC159P_18410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697470.1"
                     /translation="MQNRQRHTRPFPFPRLAGPPREGAALRLRAFGVDERDLSVDFAA
                     TARPLLVTEVLELCTESEERAAPPDPGFFWDLTVGKRIESLLAIAGGGGA"
     gene            complement(319..843)
                     /locus_tag="AC159P_18415"
     CDS             complement(319..843)
                     /locus_tag="AC159P_18415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010142636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098015 - virus tail [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HIR3697471.1"
                     /translation="MAKGFVKNANRFDPYKNFKFRVTWDGKTVLGVSKVSALKRTTEV
                     VKHREGGDNSTDRKSPGRTTYDAVTMERGLTHDPEFEKWANKVHQFAGDAAMDLVNYK
                     KELTLEMMNEKGHVVYRYFLHACWVSEFTSLPELNANANAVAIEMLKIELEGWEHDPE
                     TKEPDEASAVPQAP"
     gene            complement(862..3270)
                     /locus_tag="AC159P_18420"
     CDS             complement(862..3270)
                     /locus_tag="AC159P_18420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014086933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath family protein"
                     /protein_id="HIR3697472.1"
                     /translation="MPVQVSFPGVYIQEVPSGVHTITGVATSIAAFIGRTSKGKMNKA
                     TRILSLSDFVRAFGEPHPLSDLAQSVRLFFTNGGTDCYVIRIANGGGAASATLETLAG
                     AAALVVRAKAEGRWGDTVRLEVDYNTQNPDESFNLRVIHEEDGVAVTSESFPNLSMNP
                     ASARFAPSFVTQSSELVEVTVAPTLDINNLATSFAGYSLGRRTLNSLGNTLGDVNQLI
                     INLVTAGRSRFQISVNDGPPRPVDLSPWPLPAAPAPTEADIIAAFEGRINPALSGLSP
                     APTVAVTIAAAGAMGRLLRITANNGAQSSVRVTSASSNDIAAALMLGIDQGGVEPTRY
                     SNFRPAPTATFLPLGAAATPGDVSLLNTISNFAQNNLTGLTIDGTAVGPFSLQTTAAP
                     TDAWFQSAPVVGSPHANNDGVREKLRIIAGAINGTAGLPYRAEVWGYHLAIIARNGPF
                     NQMPAPIVSAPALAALTTPLNDGRNWRQYTLGTAGGGPVSTAGAGGDDGTAPLAADYL
                     GNPVPQTGLRALDPVDIFNLLVIPADGQVPEATYLDVIGGASTYCAERRAFLLVDGPP
                     SWTVNNRPKVVEDTTKVDNDVRKRVVKINSAVFYPRLVFSDNGLIKRIGPSGAIAGLM
                     ARIDSSRGVWKAPAGTEADLRGIVGLEVVLTDAENGVLNKIGVNCERSFPSGLVNWGA
                     RTLDGHDDFGSEWKYIPIRRLALFLEESLFRGTKWIVFEPNDEPLWAKIRLNLNAFMM
                     SLFRQGAFQGSTPDKAFYVKCDAETTTQNDRNLGIVNIEVGFAPLKPAEFVIIKIQQI
                     AGEL"
     gene            complement(3307..3594)
                     /locus_tag="AC159P_18425"
     CDS             complement(3307..3594)
                     /locus_tag="AC159P_18425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697473.1"
                     /translation="MCISDKSQIARQVLSYLSEHAGAQDTLEGIVEWWLLPQRIRERS
                     AAVRAALDDLADQKLIVASAGRDARVHYRINRRKMKAIRALLEQPEGGGNA"
     gene            complement(3914..5305)
                     /locus_tag="AC159P_18430"
     CDS             complement(3914..5305)
                     /locus_tag="AC159P_18430"
                     /inference="COORDINATES: protein motif:HMM:NF012385.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 interaction domain-containing protein"
                     /protein_id="HIR3697474.1"
                     /translation="MRQANILLLDCCTESHLADSLWDTLAPAFTPGAHLRRGLRPERP
                     GGWPAEAADFEPQLVFLTLDGGELPRAVDTVESLRRGRAETAIMLVVDGWEAVEVLEL
                     LRHGAADFITPPLRDADILPRVWRLLEHLSWRGDPTRALTEKIGLKRLVGEAPNFVSE
                     IGKIPLVARTDGRVLITGATGTGKELCARAIHYLSPRAGRPFVPVNCGAIPVELVENE
                     LFGHERGAYTGATHAQHGLIHEARGGTLFLDEIDCLPLMAQTKLLRFLQDHEYRPLGS
                     ARVRRADVRVIAASNVNLEEAVREGRVRRDLYYRLNIIQLVLPPLRERREDIPLLARH
                     FLAEYAAEFDRPAADISPDAMQALVSYDWPGNVRELEHAIERAVMLCGAEVLGVGDIL
                     LPGRVDAGDESFRQAKARIVADFEKSYVEHLLVSHEGNISGAARAAHKNRRAFWQLIR
                     KHRIDVRRFKHHS"
     gene            complement(5569..>6819)
                     /locus_tag="AC159P_18435"
     CDS             complement(5569..>6819)
                     /locus_tag="AC159P_18435"
                     /inference="COORDINATES: protein motif:HMM:NF046388.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HIR3697475.1"
                     /translation="FLRWPGGNVAQDYRWPWATGPRDERFTWVNLSWKNEPEPGDFGP
                     DEYIGCARAVGAEPTITVHVEGRGANAEEAAAWVEYCNGPATSKYGAMRAANGHPAPY
                     NIKLWEIGNEIWGNWVRGHSNAETYARNYVAYVRAMRAVDPSLKFIAVGDNDMQWNRT
                     VLARAGADIDYLAIHHYYGRREMEGDALNLMARPLHFERFYGEVEKLMKELVPGRPIK
                     LAINEWGLDLPSERQYSMESALYGARLMNVFERRGELVEMSAVSDLVNGWPGGIIQAG
                     RQKVFVSPLYHVNRLYNEHRGDERLAATASGPAFDTSKEGRGVPHLDAVASRTADGRK
                     IFIKAVNTDPVHALATTITVVGATPAARAEVLTLAAPSLRTSNDFARPEAVSVRRATL
                     RAGRSFVVHLPKHSVSVIALDVAR"
BASE COUNT          973 a         2205 c         2471 g         1170 t
ORIGIN      
        1 cgcgccgccg ccgcccgcga tggcgagcag cgactcgatc cgtttgccga ccgtcaagtc
       61 ccagaagaat ccggggtcgg gcggcgcggc gcgctcctcg ctctcggtgc acaactccag
      121 cacctccgtg acgagcaggg gccgcgccgt cgccgcgaag tccacggaga ggtcgcgctc
      181 gtccacgcca aaggcgcgaa ggcggagcgc cgccccttcg cgcggcggcc cggcgagacg
      241 cgggaacggg aaggggcgcg tgtgtcgctg ccggttctgc atcggtgttg cggccacgcg
      301 ggcgagagcg cgcgcgcgtt agggcgcttg cgggacggcg ctggcctcgt ccggctcctt
      361 cgtctccggg tcgtgctccc agccctcaag ctcgatcttg agcatttcga tggcgacggc
      421 gttggcgttg gcgttcagct cgggcagcga ggtgaactcg ctgacccagc aggcgtgcag
      481 gaagtagcgg tagacgacgt gccccttctc gttcatcatt tcgagcgtca actctttctt
      541 gtagttgacg aggtccatcg ccgcgtcgcc cgcgaactgg tgcaccttgt tggcccactt
      601 ctcgaactcg gggtcgtgcg tcagcccgcg ctccatcgtc acggcgtcgt aggtggtgcg
      661 ccccggcgac ttgcggtcgg tcgagttgtc gccgccctcg cggtgtttga ccacctcggt
      721 cgtccgcttg agcgcgctca ccttgctcac gccgaggacg gtcttgccgt cccacgtgac
      781 gcggaacttg aagttcttgt aggggtcgaa gcggttagcg ttcttgacga atccttttgc
      841 catgcacctg ttcctcccgc gttagagttc gcccgcgatc tgctgaatct tgatgatgac
      901 gaactccgcc ggcttgagcg gcgcgaagcc gacttcgatg ttgacgatgc cgaggttgcg
      961 gtcgttctgc gtcgtcgtct cggcgtcgca cttgacgtag aaggctttgt cgggcgtgct
     1021 gccctggaac gcgccctgcc ggaagaggct catcatgaag gcgttgaggt tgagcctgat
     1081 cttggcccac agcggttcgt cgttcggctc gaagacgatc cacttcgtgc cgcggaacag
     1141 agactcttcg aggaagagcg cgaggcggcg gatggggatg tacttccact cggagccgaa
     1201 gtcgtcgtgg ccgtcgagcg tgcgcgcgcc ccagttgacc agccccgacg ggaacgagcg
     1261 ctcgcagttg acgccgatct tgttcagcac gccgttctcc gcgtcggtga ggacaacttc
     1321 gaggccgacg atgccgcgca ggtcggcctc cgttccggcg ggcgccttcc agacgccgcg
     1381 cgacgagtcg atgcgcgcca tcagcccggc gatggcgccc gacgggccga tccgcttgat
     1441 caacccgttg tcgctgaaga cgagacgcgg gtagaagacc gccgagttga tcttgacgac
     1501 ccgcttgcgc acgtcgttgt ccaccttcgt cgtgtcctcg accaccttcg ggcggttgtt
     1561 gaccgtccag ctcggcggcc cgtccacgag caggaaggcg cggcgctcgg cgcagtaggt
     1621 gctcgccccg ccgatcacgt cgaggtaggt cgcctcgggc acctgcccgt cggcggggat
     1681 gacgagcagg ttgaagatgt ccacggggtc gagcgcgcgc agacccgtct gcgggaccgg
     1741 gttgccgagg tagtcggcgg cgagcggggc agtgccgtcg tcgccgcccg cgccggctgt
     1801 gctgaccggg ccgccgcccg cggtgccgag cgtgtactgc cgccagttgc gcccgtcgtt
     1861 gagcggggtg gtcagcgcgg cgagggccgg cgcgctgacg atgggcgcgg gcatctggtt
     1921 gaaggggccg ttgcgcgcga tgatggcgag gtggtagccc cagacctccg cgcggtaggg
     1981 cagccccgcc gtgccgttga tggcgcccgc gatgatccgc agcttttcgc gcacgccgtc
     2041 gttgttggcg tgcgggctgc cgaccaccgg cgcgctctgg aaccatgcat cggtcggggc
     2101 ggcggtcgtc tgaaggctga aggggccgac cgcggtgccg tcgatggtca ggcccgtcag
     2161 gttgttctgc gcgaagttgc tgatcgtgtt caggagcgac acgtcgcccg gcgtggccgc
     2221 cgcgccgagc ggcaggaagg tcgcggtggg cgccgggcgg aagttgctgt agcgcgtcgg
     2281 ctcgacgccg ccctgatcga tgccgagcat gagcgccgcc gcgatgtcgt tcgaggaggc
     2341 gctggtcacg cgcacgctcg actgcgcgcc gttgttcgcg gtgatccgca ggaggcgccc
     2401 catggcgccc gcggcggcga tggtgacggc gaccgtcggg gccgggctga ggccggagag
     2461 cgccgggtta atcctgccct cgaaggcggc gatgatgtcg gcctcggtgg gggcgggcgc
     2521 ggcgggcagc ggccacgggc tgagatcgac ggggcgggga gggccgtcgt tgacgctgat
     2581 ctggaaccgc gagcggcccg cggtgacgag gttgatgatg agctggttga catcgccgag
     2641 cgtgttgccg agcgagttga gggtgcggcg cccgagcgag tagccggcga agctggtcgc
     2701 gaggttgttg atgtcgagcg tgggcgcgac cgtgacctcg accagctcgg agctttgcgt
     2761 gacgaaggac ggggcgaagc gcgccgaggc cgggttcatc gagagattcg ggaaactctc
     2821 gctcgtgacg gccacgccgt cctcctcgtg gatgacgcgc aggttgaagg actcgtccgg
     2881 gttctgcgtg ttgtagtcca cttcgaggcg caccgtgtcg ccccagcgcc cctcggcctt
     2941 cgccctgacg acgagggccg ccgcgcccgc gagcgtttcg agggtggccg aggcggcgcc
     3001 gccgccgttg gcgatgcgga tgacgtagca gtccgtgccg ccgttggtga agaagagccg
     3061 gacgctctgc gccagatcgc tcagcgggtg cggctcgccg aaggcgcgga cgaagtccga
     3121 gaggctgagg atgcgcgtcg ccttgttcat cttgcccttc gacgtgcgcc cgatgaaagc
     3181 ggcgatggag gtcgcgacgc ccgtgatggt gtgcaccccg ctggggactt cctgaatgta
     3241 gacaccaggg aaactgacct gtactggcat tctctgattc ctccaccggc cagccgcgcg
     3301 ccggcctcaa gcgttgccgc cgccctccgg ctgttcgagc agcgcgcgga tggccttcat
     3361 cttgcggcgg ttgatgcggt agtggacgcg cgcgtcgcgc cccgcgctcg cgacgatcag
     3421 cttctggtcg gcgaggtcgt cgagcgccgc gcgcaccgcc gccgagcgct ctctgatccg
     3481 ctgcggcagc agccaccact cgacaatccc ttcgagcgtg tcctgcgccc cggcgtgctc
     3541 cgacaggtac gacaggactt ggcgcgcgat ttgggacttg tcgctgatgc acatatcgta
     3601 tcgaaaccgt gtgtgtctgt cgggccgacg aagtgcaact ttattgccgc gcgcgcggag
     3661 ccgaatttgg cggccccacg cgcgcggcct cggtcaaata tgtccgcggg cggacgattg
     3721 agggcagcgg aacaggtcgg tcggacgcgg gttttataca gacgggaggc gtgcgagttg
     3781 aggcgcgcgt agcttaggac aaaagcgtcc taagccgcgc acgcgtttgt cctaccccgg
     3841 agcggattgc ggttgagttt acctggcgcg aagcaggacg acgttgtcgg gtgtctttcc
     3901 gcgagcgtgc tcgtcaggag tggtgtttga agcggcgaac gtcgatgcgg tgtttgcgga
     3961 tgagttgcca gaaggcgcgg cggttcttgt gcgcggcgcg cgcggcgccg ctgatgttgc
     4021 cctcgtgcga gacgagcagg tgctcgacgt agctcttctc gaaatcggcg acgatgcgcg
     4081 ccttcgcctg ccggaacgac tcgtcgcccg cgtccacgcg tccgggcagc aggatgtcgc
     4141 cgacgcccag cacctccgcc ccgcagagca tgacggcgcg ctcgatggcg tgttcgagtt
     4201 cgcggacgtt gcccggccag tcgtaggaga cgagcgcctg catcgcgtcg ggcgagatgt
     4261 ctgcggcggg gcggtcgaac tcggcggcgt actcggcgag gaagtggcgg gccagcagcg
     4321 ggatgtcttc gcgccgctcg cgcagagggg gcagcacgag ctggatgatg ttcagccggt
     4381 agtagaggtc gcggcgcacg cgcccttcgc gcacagcctc ttcgaggttc acgttcgagg
     4441 cggcgatgac gcgcacgtcc gcgcgccgca cgcgcgcaga gccgagcggc ctgtattcgt
     4501 gatcctgcaa gaagcgcagc agcttggtct gcgccatcag cggcaggcag tcgatctcgt
     4561 cgaggaagag cgtgccgccc cgcgcctcgt ggataagacc gtgctgcgcg tgtgtggccc
     4621 ccgtgtaggc gccgcgctcg tggccgaaca gctcgttctc caccagctcg acggggatgg
     4681 cgccgcagtt gaccggcacg aagggccggc ctgcgcgcgg gctgaggtag tggatggcgc
     4741 gggcgcacag ctctttgccc gtgccggtcg caccggtaat cagcaccctg ccgtcggtgc
     4801 gcgcgacgag cgggatttta ccgatctcgg agacgaagtt cggcgcctcg ccgacgagcc
     4861 gcttgaggcc gatcttctcc gtcagcgcgc gcgtcgggtc gccgcgccag gagaggtgtt
     4921 cgagcagccg ccagacgcgg ggcaggatgt ccgcgtcgcg gagcggcggc gtgatgaagt
     4981 ccgccgcccc gtgccgcagc agttcgagca cctcgacggc ctcccatcca tcgacgacca
     5041 gcatgatggc cgtctccgcg cggcccctcc tgagcgattc gacggtgtcc accgcgcgcg
     5101 gcaactctcc cccgtccagc gtcaggaaca cgagctgcgg ctcgaagtct gcggcctccg
     5161 cgggccagcc tccggggcgc tcgggccgaa gaccccggcg caggtgcgcg cccggcgtga
     5221 aggcgggcgc gagggtgtcc cacagagagt ccgcgaggtg gctctccgta cagcagtcga
     5281 gcagcaagat gttcgcctgt ctcatggcgg ctgcctacct cctgacttgg atgcggtcaa
     5341 gtgcgaggag tccgcggcag gtttcagacc atcgctggtg aagccggtgc ggtcggggca
     5401 agtgggccac gcgcgggcgt tcggcaagag gctacaagga actatggggg ccgggagtat
     5461 agcacaagtc gcggagaggg caacgacatc gttcgccttc gacgtttaac gcggaagcga
     5521 ctaaaaagac acggccattg cacgtctttg gcggcgcctg cggtgccttc accgcgccac
     5581 gtcgagggcg atgacggaga cggagtgctt cggcaggtgg acgacgaagc tgcgaccggc
     5641 gcgcaacgtc gcgcgcctga cggagacggc ttcgggccgc gcgaagtcgt tcgaggtgcg
     5701 cagcgaaggg gccgcgagcg tcaacacctc cgcgcgcgcg gcgggcgtcg cccctacgac
     5761 cgtgatggtc gtcgcgagcg cgtgcacggg gtctgtgttc acggccttga tgaaaatctt
     5821 cctgccgtca gccgtgcggc tcgccacggc gtcgaggtgc ggcacgcccc tcccttcctt
     5881 gctcgtgtcg aacgcggggc cgctcgccgt cgcggccagc cgctcgtcgc cccggtgctc
     5941 gttgtagagc cggttgacgt ggtagagggg cgagacgaag accttctgcc gccccgcctg
     6001 gatgatgccg ccgggccacc cgttgacgag atcggagacg gcgctcatct cgaccagctc
     6061 gccccgccgc tcgaagacgt tcatcagccg cgcgccgtag agcgccgact ccatcgaata
     6121 ctgtcgttcc gaagggaggt cgagacccca ctcgttgatc gcgagcttga tcgggcgccc
     6181 cggcaccagc tctttcatca acttttcgac ctcgccgtag aagcgctcga agtggagcgg
     6241 gcgcgccatc aggttgaggg cgtcgccctc catctcgcgc ctgccgtagt agtggtgaat
     6301 cgcgagatag tcgatgtccg ccccggcgcg cgcgagcacg gtgcggttcc actgcatgtc
     6361 gttgtcgccg acggcgatga acttcaatga aggatcgacg gcgcgcatcg cgcggacgta
     6421 agcgacgtag ttgcgcgcgt aggtttcggc gttcgagtgg ccgcgcaccc agttgcccca
     6481 gatttcgttg ccgatctccc agagcttgat attgtaaggc gcggggtggc cgttcgccgc
     6541 gcgcatcgcg ccgtacttcg aagtggccgg gccgttgcag tattcgaccc acgcggcggc
     6601 ctcttccgcg ttggcgccgc gcccctcgac gtggaccgtg atggtcggct cggcgccgac
     6661 ggcgcgcgcg cacccgatgt actcgtccgg gccgaagtcg cccggctcag gctcgttctt
     6721 ccaggagagg ttgacccacg tgaagcgctc gtcgcgcggc cccgtggccc acggccagcg
     6781 gtagtcctgc gcgacgttgc cgcccggcca gcgcaggaa
//
LOCUS       DBNGOH010000946         2411 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1426303, whole genome shotgun sequence.
ACCESSION   DBNGOH010000946 DBNGOH010000000
VERSION     DBNGOH010000946.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2411)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2411)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2411
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1426303"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(243..713)
                     /locus_tag="AC159P_18440"
     CDS             complement(243..713)
                     /locus_tag="AC159P_18440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697476.1"
                     /translation="MKRLSLIVALCLTAGLLPPFVARTATNYSDTVSGFQTATAEGVA
                     VVVDAAGDLPGMGKVTLRHEGGNVTGGSWTLTVLPPGADASASERGKLSGAVAGGTVT
                     FKPDGTLAGMSAVQLTIEGGTGQYADVSGGTGTLNLSADAERPSQFNGTLVLDF"
     gene            1738..>2411
                     /locus_tag="AC159P_18445"
     CDS             1738..>2411
                     /locus_tag="AC159P_18445"
                     /inference="COORDINATES: protein motif:HMM:NF025092.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HIR3697477.1"
                     /translation="MPSPTRFLRKLGAPGLCLLLMPVLLVAGAPVARAGTMTAAPVAP
                     AVSIKGTVIDQAGAAVSGAAVTLSTATSAELARAATDAHGEFSFAAVAANAYVLAVSK
                     DGFAEARQRLSPAPGQSVSLRIQLRVGPLMESVTVTPARGQAREMFETPEAVGVLTAE
                     DISRRAHFILPQALKEEPGVHLQQTTTSQGSLFLRGLTGQQVLNLIDGVRFNNSTFRP
                     GANQYTA"
BASE COUNT          411 a          768 c          822 g          410 t
ORIGIN      
        1 atgggcgcca tggtctggag caggaggcag aagatgagga gctgcgcgac gacgcggcgc
       61 gcgcggaaca tgacggcttc gacggaggaa gacttcttcg ctgctctgct tttcatggga
      121 gatgatcctt ttcggttatc aagcttgcgg caagcttctg gtcgaagccg tgaacgttca
      181 ccgtgggcgc gcatcgccgg aagtcgcggg cgcggcggac ggaagggcgc gaggtcggtc
      241 gatcaaaagt cgaggacgag ggtgccgttg aactgcgacg gcctctccgc gtcggcggaa
      301 aggttgaggg tgccggtgcc gccgctcacg tcggcgtact gcccggtgcc gccctcgatg
      361 gtcagttgca ccgcgctcat gccggcgagc gtgccgtcgg gtttgaaagt gaccgtgccg
      421 ccagcgaccg cgcccgacaa cttgccccgc tcgctggccg aagcgtcggc ccccggcggg
      481 agcaccgtca gcgtccaact gccgccggtc acgttgcccc cttcatgccg gagggtgact
      541 ttgcccatcc ccggcaggtc gcccgcggcg tccaccacca cggccacgcc ctccgcggtc
      601 gcggtctgaa agccgctcac ggtgtcgctg tagttggtgg ccgtgcgcgc gacgaagggc
      661 gggagcagcc ccgccgtcag acacaaggcg acgatcagag aaagacgttt catcacaatc
      721 ctccgtgccc gcggctcgca gccgcgcgtg ggatcgaccg cgccgccgac gacgggcgcg
      781 gcgtcggcgg gtcgggagtt ctgttcgatt gtttgtcgag tcgggcggcg ggcagggaag
      841 cgccgggcgt cgcgcccggc gtgagaagcg tcgtgtgaga agtgagtaag ccggcgcgta
      901 cgcgcgccgc actacacgtc atggggggga actctttctt gcctgcgccg ccgccgccgc
      961 gccggaacgc gtgccgaccc gccgcgcttt tcgcggtagc agagcggggg tgccgcgtag
     1021 gagatgtgga tagactaagc cccgaactta agacagggga tcgtgtggct cttgctatga
     1081 acgagggctg ctgcggctac gaatgcgagt agccgggtaa cacacgtcgg gttgttgtcg
     1141 tcagggaaca ggccaacagc agggttatgt cagacgagaa tttgaggctc ggcatgacgg
     1201 ttcaaagtta tggggcaggc gcacagtaga caaaatcggc gagtctgtca agaccttctt
     1261 tcgcgacctc cggcgagcgc cgccggaggt cgcgcgccgc gctcgcgcgg gcgttaacaa
     1321 catcatctta taatgcgcga atgaaatttt cgttaagcga aaaagaactt cttgacaacc
     1381 gccgaacgtg tctgtagtgt gtcgccttca actcagacgg tagttagttc ggttgcccca
     1441 gcaagtcctt cacagtcatc cgcagcgtaa gtcagtcgtc atcgttcacc gcccctcgcc
     1501 gagctttttc atcggcacgc gtccgcgcgc gatgatgtag agccgaactc ggcggcaggc
     1561 atcggacccg acagcccgac attcatcggg aagctgtcgc tttgcgcaca cacgcacacg
     1621 gcctgcttga tcgctcctcg tgccgagttc cgcgctgacg aatccctgag ttagacacac
     1681 gctctgataa tccaccgata gaagaacacc cgcatcgcag gtacaggagt catgtccatg
     1741 ccatcgccga cccgcttctt acgaaagctt ggtgcgcccg gattgtgtct gctgctgatg
     1801 ccggtgctgc ttgttgcggg cgcgcccgtc gcgcgggccg gcacgatgac tgcggcgcct
     1861 gtcgcgcccg ccgtgagcat caaggggact gtcatcgacc aggcgggggc cgccgtcagc
     1921 ggcgccgccg tcacgctctc gacggcgacg agcgcggagc tggcgcgcgc ggcgacggac
     1981 gcgcacggcg agttctcgtt cgcggcggtg gccgccaacg cctacgtcct cgccgtcagc
     2041 aaagacgggt tcgcagaggc gcggcagaga ctctcgcccg cgcccggcca gagcgtgtcg
     2101 ctgcggattc agctccgcgt cgggccgctg atggagtcgg tgacggtgac gcccgcgcgc
     2161 gggcaggcgc gcgagatgtt cgagacgccc gaagcggtcg gcgtgttgac ggcggaagac
     2221 atctcgcgcc gtgcgcactt catcctgccg caggcgctca aggaagagcc gggcgtccac
     2281 cttcagcaga cgacgacgag ccagggttcg ctcttcctgc gcgggctgac gggccagcag
     2341 gtcttgaacc tcatcgacgg cgtgcgcttc aacaactcca ccttccgacc gggcgcgaac
     2401 cagtacacgg c
//
LOCUS       DBNGOH010000947         5701 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_510048, whole genome shotgun sequence.
ACCESSION   DBNGOH010000947 DBNGOH010000000
VERSION     DBNGOH010000947.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5701)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5701)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5701
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_510048"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            136..1614
                     /locus_tag="AC159P_18450"
     CDS             136..1614
                     /locus_tag="AC159P_18450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIR3697478.1"
                     /translation="METYRNFIGGQWVASSSAKQVANINPANTDDVLGHAPLSTREEA
                     RRAVESAAEAFRAWRSTPAPARGRIVARAARLLEDNKEELARLLTREEGKTVAESRGE
                     LQRAANVADFCAGEARRLNGETIQSELPSNFAYTIRQPHGVVACVTPWNFPVAIPVWK
                     IAPALVAGNTVVFKPATLTPATAVRIVEIFSEAGLPAGVLNLILGSGSDAGDEIVGHP
                     AVRAVSFTGSNGVGLNLYEQCARRGAKVQCEMGGKNPVVVLEDADMELAVESTAQGAF
                     GSTGQRCTATSRAVVVDEVADEFLERIVARAESLKLGDGLDPATDVGPSVDEKQFETV
                     LRYIDIGREDGAKLACGGTRADTDHLSKGYFVRPTVFADVTADMRLAREEIFGPVLSV
                     LRVKDFEEALRVANDSEYGLSSSIFTNDAARVFRFVDEIETGMTHINSPTTGGEAHIP
                     FGGIKATGIGEREQGSTALDFYTELKVVYVDYTGRKREGNLY"
     gene            1637..2650
                     /locus_tag="AC159P_18455"
     CDS             1637..2650
                     /locus_tag="AC159P_18455"
                     /inference="COORDINATES: protein motif:HMM:NF025910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3697479.1"
                     /translation="MKARLFPLTFGFVLACALAAAARQSERQPTHRVALPGKDWALNV
                     ALPDLKVQEVDGDPGTNEFYLVATRPDVKRSPVLMLRLMPAQRAGGPEVVRDFSLGAL
                     KKSDLTKERDVRTSAYGQVPVARYTQAIQLVPTVTLFEGMKCAEGYFVRDGFWVTVKL
                     MSQSFGDKEESLFHSVLDSVRFADTTRPADSFDYFHKGRAAYRAADYVRAVGHYRAAF
                     ELERKGRRLPPDSWRALVEQLANAYGALSDYASAKEVLEYGLANDPTHFWFNYNLARV
                     YAAAGDADNAVAYLEKAALVHKQADRRRFSLGGPPPDPSKDAVFARLKETDKFRKAAK
                     ALR"
     gene            2720..4027
                     /locus_tag="AC159P_18460"
     CDS             2720..4027
                     /locus_tag="AC159P_18460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HIR3697480.1"
                     /translation="MSTAEQKTAASETVRKHKEFLFPAVATYYQEPLALVRGEGEHVW
                     DDQGNRYLDCFGGVLTVSVGHANPQVNAAIIEQVKTISHTSTLYCNKPQSDLAEKLHQ
                     ITPGRLKKSFFTTSGTEADDTAIHAAKTATGRHEIVVLRHSYSGRSATALSAIGHSTW
                     RSLPSQVAGIVHARAPYCYRCPLKLSYPECGLACAEDIEDVIMTTTTGEIAAFMAEPI
                     LGVGGFIVPPQGYFERAAEITRKHGGLFIADEVQTGWGRTGDKWFGIEHWNVEPDIIT
                     SAKGMGNGVPLGMTTATPEVADKYPGLTFSTFGGNPVSMAAALAVIKVIEDENLRSNA
                     ATLGAYFRQRLEELKDKHTVVGDVRGMGLMQALELVKDRETKEPAPQAVLQVFEETKR
                     RGVLIGKGGLYGNVIRTGVMLNSTKDTIDELVAALDAGLATLV"
     gene            4040..4273
                     /locus_tag="AC159P_18465"
     CDS             4040..4273
                     /locus_tag="AC159P_18465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697481.1"
                     /translation="MNHLSLAALLIRLFGLGMMLFPAADLTRCAVRSPWALLNPFNYI
                     DLTGGLIVGLLLMAVSKPLAAPICRGLDGAGLK"
     gene            4325..4561
                     /locus_tag="AC159P_18470"
     CDS             4325..4561
                     /locus_tag="AC159P_18470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697482.1"
                     /translation="MCRLCSALFFWLVTLGPFVLVGVSVFLAVFGKAYGNFPYQPDET
                     RAFKKYALPRAHLRCFQFFDRRKPELKPARHRRK"
     gene            4558..>5701
                     /gene="hydA"
                     /locus_tag="AC159P_18475"
     CDS             4558..>5701
                     /gene="hydA"
                     /locus_tag="AC159P_18475"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018052905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004157 - dihydropyrimidinase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HIR3697483.1"
                     /translation="MKTLITNGRVVTAVDDYKADILVEGETVSVIGAKLEMEADRVID
                     AAGKLVIPGGIDPHTHMELPFGGTQASDDFRTGTVAAAHGGTTTIIDFAVQYKGQALL
                     EAVDNWHAKAEGKTAIDYGFHLITTELEDNQIEEMYTVMDEGVTSFKLFMAYPGVFLV
                     DDATIFRAMSAAGARGGLVCMHAENGIVINEIIKRALAEGRTSPKYHALTRPTIAEAE
                     GVHRAIRLAEMAESPVYIVHLSCADALHQVREARDRGLPAFAETCPQYLFLSIDNYDE
                     PDFGGAKYVMTPPLREKWNQDELWKGLKTDDLQVISTDHCPFCMKEQKELGRDDFSKI
                     PNGAPGVEHRMSLIYDGGVAAGRVSLNRFVELTSTAAARMFGLFPRK"
BASE COUNT         1023 a         2046 c         1756 g          876 t
ORIGIN      
        1 ccgtcgcagt cagtcccgcg ggcagcaagc ggcgcgctcc ccagcttatc gaacacgctt
       61 ccatttccac aacggcttcg gggcttagag gactgtcgcg cgcccgattc tacatcgaca
      121 gtcaaccgca accttatgga gacctatcgc aacttcatcg gcggtcagtg ggtcgcgtca
      181 tcatccgcga aacaggtcgc aaacatcaac cccgcgaaca ccgacgacgt gctcggccac
      241 gccccgcttt cgacgcgcga ggaggcccgc cgcgccgtcg agtccgccgc cgaagccttc
      301 cgcgcgtggc gctcaacccc cgcgcccgcg cgcgggcgca tcgtcgcacg ggccgcgcgc
      361 ctgctcgaag acaacaagga agagttggcc cgcctgctca cgcgcgagga gggcaagacc
      421 gtggccgagt cgcgcggcga attgcagcgc gcggccaacg tcgccgactt ctgcgcaggc
      481 gaggcgcggc ggctgaacgg cgagacgatt caatccgagc tgccctcgaa cttcgcctac
      541 accatccgcc agccgcacgg cgtcgtcgcc tgcgtcacgc cgtggaactt ccccgtcgcc
      601 atccccgtct ggaagatcgc gcccgcgctc gtcgccggca acaccgtcgt cttcaagccc
      661 gcgacgctga cgcccgccac cgccgtccgc atcgtcgaaa tcttcagcga agccgggctg
      721 cccgcaggcg tactcaatct gattctcggc tccggctccg acgcgggcga cgagatcgtg
      781 ggccacccgg cggtgcgcgc cgtctccttc acaggctcga acggcgtggg gctgaacctc
      841 tacgagcagt gcgcgcggcg cggcgcgaag gtgcagtgcg agatgggcgg caagaacccc
      901 gtcgtcgtgc tcgaagacgc ggacatggag ctggccgtcg aatcgaccgc gcagggcgcc
      961 ttcggctcga ccggccagcg ctgcacggcg accagccgcg ccgtcgtcgt cgatgaggtc
     1021 gccgacgaat tcctcgaacg catcgtcgcc cgcgccgaaa gtctcaaact cggcgacggc
     1081 ctcgaccccg cgaccgatgt cggcccctcg gttgacgaga agcagttcga gaccgtgctc
     1141 cgctacatcg acatcgggcg cgaggacggc gcgaagctcg cttgcggcgg cacgcgcgcc
     1201 gacaccgacc acctctccaa aggctacttc gtccgcccca ccgtcttcgc cgacgtgacg
     1261 gcggacatgc gcctcgcgcg cgaagagatt ttcggccccg tgctctcggt gctgcgcgtc
     1321 aaggacttcg aagaggcgtt gcgcgtggcg aacgacagcg agtacggcct ctcctcctcg
     1381 atcttcacca acgacgcggc gcgcgtcttc cgcttcgtgg acgagatcga gaccggcatg
     1441 acgcacatca actcgccgac caccggcggc gaggcgcaca tccccttcgg cggcatcaag
     1501 gcgaccggca tcggcgagcg cgagcaaggc tcgaccgcgc tcgacttcta caccgagctg
     1561 aaggtcgtct acgtcgatta cacgggccgc aagcgcgagg gcaacctcta ttgaccccgc
     1621 gccgccggag tcgaagatga aggcacggct gttcccactc acgttcggtt tcgtcctggc
     1681 gtgcgcgctc gccgcggcgg cgcggcagtc cgagcgccag ccgacgcacc gcgtcgccct
     1741 gcccggcaag gactgggcct taaacgtggc gctgcccgac ctgaaagttc aagaggttga
     1801 cggcgacccc ggcaccaacg agttctacct cgtggcgacg cggcccgacg ttaagcggtc
     1861 gccggtgctc atgctccgcc tgatgcccgc gcagcgggcg ggcggccccg aagtcgtcag
     1921 agacttcagc ctcggcgcgc tgaagaagtc cgacttgaca aaagagcgcg acgtgagaac
     1981 ctcggcttac gggcaggttc ccgtcgcccg atacacgcag gccattcaac tcgtcccgac
     2041 ggtcacgctg ttcgagggga tgaagtgcgc cgaaggctac ttcgtcaggg acggcttctg
     2101 ggtgaccgtc aagttgatga gccagtcttt cggcgacaag gaggagagcc tgttccactc
     2161 cgtcctcgac tcggtcaggt tcgccgacac gacgcgcccc gccgacagct tcgattactt
     2221 ccacaagggc cgcgcggcgt acagggccgc cgactacgtc agggccgtcg ggcactaccg
     2281 cgcggccttc gagcttgagc ggaaggggcg gcggctgccg cccgactcgt ggcgcgcgct
     2341 ggtcgaacaa ctcgccaacg cctacggcgc gctgagcgat tacgcgagcg cgaaggaggt
     2401 tctggagtac gggctggcga acgaccccac gcacttctgg ttcaactaca acctcgcgcg
     2461 cgtctacgcc gcggcgggcg acgccgacaa cgccgtcgcc tatctggaga aggccgcgct
     2521 ggttcacaag caggctgacc ggcggcggtt ttcgctcggc ggcccgccgc ccgacccttc
     2581 gaaagacgcg gtcttcgcgc gccttaaaga gacggacaag ttcaggaagg cagcgaaggc
     2641 tttgaggtag cgccgggccg ccctttcgct cgcgcgccgc gcgttcactt cattttcaca
     2701 agacaggacg accacagaca tgagcacagc cgaacagaaa accgccgcct ccgagaccgt
     2761 ccgcaagcac aaggagtttc tcttccccgc ggtcgccacc tactaccagg agccgctcgc
     2821 gctcgtgcgc ggcgaaggcg agcacgtctg ggacgatcag ggcaaccgct acctagactg
     2881 cttcggcggc gtgctcaccg tcagcgtcgg ccacgccaac ccgcaggtca acgcggccat
     2941 catcgagcag gtcaagacca tctcgcacac ctcgacgctc tactgcaaca agccgcagtc
     3001 cgacctcgcc gaaaagctcc accagatcac gcccggtcgc ctcaagaaat ccttcttcac
     3061 caccagcggc acggaggccg acgacaccgc catccacgcc gccaagaccg cgaccgggcg
     3121 ccacgagata gtcgtcctgc gccacagcta ctcgggccgc tccgcgaccg cgctctccgc
     3181 catcggccac tcgacgtggc gctcgctccc ctcgcaggtc gcaggcatcg tccacgcgcg
     3241 cgcgccttac tgctaccgct gccctctgaa gctctcctac cccgaatgcg ggctggcctg
     3301 cgccgaagac atcgaggacg tgatcatgac gaccacgacc ggcgagatcg cggccttcat
     3361 ggccgagcct atcttgggcg tcggcggctt catcgtcccg ccgcagggct acttcgagcg
     3421 cgccgcagag atcacgcgca agcacggcgg cctctttatc gcagacgagg tgcagaccgg
     3481 ctggggccgc accggcgaca agtggttcgg catcgagcac tggaacgtcg agcccgacat
     3541 catcacctcc gccaagggca tgggcaacgg cgtccccctc ggcatgacga ccgccacgcc
     3601 cgaggtcgca gacaagtacc ccggccttac cttctcgacc ttcggcggca accccgtctc
     3661 gatggccgcc gcgctggccg tcatcaaagt catcgaggac gagaacctgc gctcgaacgc
     3721 cgccaccctc ggcgcctact tccgccagcg cctcgaagaa ctcaaagaca agcacacggt
     3781 cgtcggcgac gtgcgcggca tgggcctcat gcaggccctc gaactcgtca aagaccgcga
     3841 gaccaaagaa cccgccccgc aggccgtcct ccaggtcttc gaagagacca agcggcgcgg
     3901 cgtcctcatc ggcaagggcg gcctctacgg caacgtcatc cgcacgggcg tcatgctcaa
     3961 ctcgacgaag gacaccatcg acgaactcgt cgccgccctc gacgccggtc ttgccactct
     4021 cgtttagaag gaagcgacca tgaaccacct ctctctagcg gccctgctga ttcgcctctt
     4081 cgggctgggg atgatgctct tccccgccgc ggatttaacc cgctgcgcgg tgcggagtcc
     4141 gtgggccttg ctcaaccctt ttaattacat cgacctgacc ggcggcctga ttgtaggcct
     4201 actcctgatg gccgtgagca aaccgctcgc cgctccgatc tgccgcggcc tcgacggcgc
     4261 cggtctcaaa tgacggggcc gcaaccgcac ggcaccgacc gcgccgccgt gcgtgtaaag
     4321 tgccatgtgt cgcctctgct ccgcgctgtt cttctggctg gtcacgctcg gcccgttcgt
     4381 tctcgtcggc gtctctgtct tcctcgccgt cttcggcaag gcgtacggca acttcccgta
     4441 tcagccggac gaaacgaggg cgttcaaaaa gtatgccttg ccgcgcgcgc accttcgttg
     4501 ttttcagttt tttgatcgtc gtaaaccaga actgaaaccc gcacgtcaca gaagaaaatg
     4561 aaaacgctca tcactaacgg tcgcgtcgtc accgcggttg acgactacaa ggcggacata
     4621 cttgtcgaag gcgagaccgt ctcggtcatc ggcgcgaagc tcgagatgga ggccgaccgc
     4681 gtgatcgacg cggcgggcaa gctcgtcatc cccggcggga tcgacccgca cacgcacatg
     4741 gagctgccgt tcggggggac gcaggcgtcg gacgattttc gcaccggcac ggtcgcggcg
     4801 gcgcacgggg ggacgacgac gatcatcgac ttcgcggtgc agtacaaggg gcaggcgctc
     4861 ttggaggccg tggacaactg gcacgcgaag gccgagggca agacggccat cgattacggg
     4921 ttccacctca tcacgaccga gcttgaagac aaccagatcg aagagatgta cacggtcatg
     4981 gacgagggcg tgacgagctt caagctgttc atggcctatc cgggcgtctt tctggtggac
     5041 gacgcgacca tcttccgcgc gatgtcggcg gcgggcgcgc gcggcggcct cgtctgcatg
     5101 cacgccgaga acggcatcgt catcaacgag atcatcaagc gcgcgctggc cgagggccgc
     5161 acctcgccga agtaccacgc gctcacgcgc ccgaccatcg ccgaggccga gggcgtccac
     5221 cgcgccatcc gcctcgccga gatggccgag tcgcccgtct acatcgtcca cctctcctgc
     5281 gccgacgccc tgcaccaggt gcgcgaggcg cgcgaccgcg gcctgcccgc cttcgccgag
     5341 acctgccccc agtacctctt cctctcgatc gacaactacg acgagccgga cttcggcggc
     5401 gcgaagtacg tgatgacgcc gccgctccgg gagaagtgga atcaggacga gctgtggaag
     5461 ggactgaaga ccgatgacct gcaggtcatc tccaccgacc actgcccctt ctgcatgaag
     5521 gagcagaagg agctgggccg cgacgacttc tcgaagatcc cgaacggcgc gcccggcgtc
     5581 gagcaccgca tgtcgctcat ctatgacggc ggcgtggcgg cggggcgcgt ctcgctcaac
     5641 cgcttcgtgg agctgacctc gaccgccgcc gccaggatgt tcggcctctt cccgcgcaaa
     5701 g
//
LOCUS       DBNGOH010000948         1614 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_875622, whole genome shotgun sequence.
ACCESSION   DBNGOH010000948 DBNGOH010000000
VERSION     DBNGOH010000948.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1614)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1614)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1614
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_875622"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..727
                     /locus_tag="AC159P_18480"
     CDS             <1..727
                     /locus_tag="AC159P_18480"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HIR3697484.1"
                     /translation="LDEAAEVWSAVEEFDGRDSFAERAPSHLLERFVPGDVYHVDSLV
                     AGGRVVFASVERYGAPPFEVVHGGVSTSHTLRRESADERRLLALNRKLLAGFGFERGT
                     THAEFIKSADGGQFYFLEVAARVGGAYTAETIEAATGINPWREWARVELAGGGRPYTP
                     PPARQDYGGVAVSLARQEWPDTAAYNDPEIVYRVRKPWHVGLIVRSPVYDRVGALLEE
                     YKRRFAEDFTAAVPPEERPAQHL"
     gene            724..1530
                     /locus_tag="AC159P_18485"
     CDS             724..1530
                     /locus_tag="AC159P_18485"
                     /inference="COORDINATES: protein motif:HMM:NF015983.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HIR3697485.1"
                     /translation="MSRTSTVYGPVHSWRLGRSLGVDPLLVNSVCSFRCLYCQLGRIN
                     VHTGARKVYVPTERVMRDLGACDWRSADVITLSGSGEPTLAANVGEVIRGIKALTAKP
                     VVVLTNATTLGDPEVRRDLCEADRVFCKLDAADEETFRKINRPVAGLSLRSVVEGIKQ
                     FKSEYAGHLAVQIMLMPLNAGEAEVFAKILRAVRPDEVQLNAPSRKLPREWRVEARGN
                     SPEGLIGGARVRQVGAGEAAQFEDTMRRLAGVKTITTHPQWRLRGTRRGV"
BASE COUNT          274 a          537 c          557 g          246 t
ORIGIN      
        1 gctcgacgag gcggcggagg tctggagcgc ggtcgaagag ttcgacgggc gcgactcctt
       61 cgccgaacgc gcgccgtccc acctgctcga acgcttcgtg cccggcgacg tttaccacgt
      121 ggactcgctc gtcgcgggag gtcgcgttgt tttcgccagc gtcgagcgct acggcgcgcc
      181 cccgttcgag gtcgtgcatg gcggcgtctc cacctcgcac acgctcaggc gcgaatcagc
      241 ggacgagcgt cgtctcctcg ccctcaaccg gaagctgctg gccggcttcg gcttcgagcg
      301 cggcacgacc cacgccgaat tcattaagag cgccgacggc ggccagttct atttcctgga
      361 ggtcgcggcg cgggtcggcg gggcatacac ggcagagacc atcgaggccg ccaccgggat
      421 caacccttgg cgcgagtggg cgcgcgtcga actggcgggg ggagggcgcc cgtacacgcc
      481 gccgcctgcc cgccaagact acggcggcgt cgccgtctcg ctcgcccggc aggagtggcc
      541 cgacaccgcg gcttacaacg accccgaaat cgtctaccgc gtccgtaaac cgtggcacgt
      601 cggcctcatc gtgcgctcgc cggtttacga ccgcgtaggc gcgctgctcg aagaatacaa
      661 gcgccgcttc gccgaagact tcacggcggc ggtcccaccc gaagagcggc ccgcacagca
      721 cctatgagca ggacatcgac agtctacggc cccgtccact cctggcgcct cgggcggtcg
      781 ctcggcgttg acccgctcct cgtcaactcc gtctgctcgt tccgctgcct ctactgccag
      841 ctcggccgga tcaacgtcca cacgggcgcg cggaaagttt acgtcccgac cgagcgggtg
      901 atgcgcgacc tcggggcgtg cgactggcga agcgcggacg taatcaccct gtcggggagc
      961 ggcgagccga cgctggcggc gaatgtgggc gaagttattc gcgggatcaa agcgctcacg
     1021 gcgaagcccg tggtcgtgct gacgaacgcc acgacgctcg gcgacccgga ggtcaggcgc
     1081 gatctgtgtg aggccgaccg cgtcttctgt aaactggacg ccgccgacga agagactttc
     1141 aggaagatca accgcccggt cgcggggctc tcgctccgct cagtggtcga gggcatcaag
     1201 cagtttaaaa gcgagtacgc gggccacctc gctgtccaga tcatgctcat gccgctgaac
     1261 gcgggtgagg ccgaagtgtt cgcgaaaatc ctgcgggcgg ttcgccccga cgaggttcaa
     1321 ctgaatgcgc cgagccgcaa gctcccgcgc gagtggcgcg tcgaggcgcg cggcaactcg
     1381 ccggagggac tgatcggcgg ggcgcgcgtc aggcaggtcg gagccggcga ggccgcgcag
     1441 ttcgaggata cgatgcggcg gctcgcgggc gtgaaaacta ttacaaccca cccgcagtgg
     1501 cggctgagag gtacaagacg gggagtgtga tgtcgaacag cgtgcgtgaa aaggtcgtcg
     1561 ccgaatatca cgggatgctg tcgcgggacg agagcctgac cccagagcta ttcg
//
LOCUS       DBNGOH010000949         3827 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1426656, whole genome shotgun sequence.
ACCESSION   DBNGOH010000949 DBNGOH010000000
VERSION     DBNGOH010000949.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3827)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3827)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3827
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1426656"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1573)
                     /locus_tag="AC159P_18490"
     CDS             complement(<1..1573)
                     /locus_tag="AC159P_18490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR3697486.1"
                     /translation="MSFRQTPPALGNQYEDDRVLRSYLTRALPPEMLREIEPSLAELG
                     RLAGGDLYRLQLEDRLNEPRLTQWDAWGNRVDRIEVTPLWRVAASLAAEHGVVATAYE
                     RRHGLYSRIHQCALAYLFTPSTDIYSCPLAMTDGAARTLLDSGNRALVERAVPHLTAR
                     EPREFWTSGQWMTELTGGSDVGLTETTARRDEGGQWRLYGRKWFTSATTAETALTLAR
                     PEGNPAGGRGLALFYVEPRDEAGRLRGIQVDRLKDKLGTRKVPTAELTLRDAPAELVC
                     GTTDGVRHIAPMLNVTRLWNGLSAASLMRRGLALARDYARKRVAFGAPLAEKPLHADT
                     LAAQQAEAEAALHLAFRVAELLGLEECGEATEEQSRLLRLLTPLMKLTTARQAVAVAS
                     EVLEAFGGAGYVEDTGLPVLLRDAQVLSIWEGTTNVLALDGLGAAGPAGGPWNYLRRE
                     LEACAREATGARLAPAAAAALQALAHADAWRARAAREGQPAVEAGARRFALTVGRAAA
                     LALLARHAQWSLDNEG"
     gene            complement(1591..1815)
                     /locus_tag="AC159P_18495"
     CDS             complement(1591..1815)
                     /locus_tag="AC159P_18495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697487.1"
                     /translation="MESEISSRAELIDRRAYTLALQLEGWDIDVNGHVHANALGGDET
                     RPWPLRFRMGGWDGDALCAYVRGSWEIPLL"
     gene            2111..3241
                     /locus_tag="AC159P_18500"
     CDS             2111..3241
                     /locus_tag="AC159P_18500"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HIR3697488.1"
                     /translation="MKHVLSKHVLLAFVLVVLYQGSSLASDSGRGSAQAEGPVRIGVE
                     PRDVFVERTASAQLVSFDFLLENLGAEPARVARIELSVYDRRGALVLRKFLTAGGLRH
                     GDYEIARLEPKATAFLFNPFENFSTEVELASMRYEFFFEAAEGAREFSASVNIAPKVF
                     RPKTRLRLPLKGRVLVHDGHDFYSHHRRFNLAHPFLREIGVTHNFTRFASDLCVVNER
                     GDLRRQRSDANTDWYGFGATVYAPGAGRIVRARGDVPDNVNGKSTFTIDDFRKDATVP
                     AGNFLVIDHRNGEYSLIAHLKQGSLLVREGDTVRAGQPIGQMGISGDAYLPHVHYELR
                     SASTVNAHAFPAYFHNFTRLHGSRRTHVLQGPVDSGDLLESR"
BASE COUNT          638 a         1348 c         1246 g          595 t
ORIGIN      
        1 cgccctcgtt gtcgagcgac cactgcgcgt ggcgcgcgag cagggcgagg gcggcggcgc
       61 gcccgaccgt gagggcgaaa cggcgcgcgc cggcttcgac cgccggctgc ccctcacggg
      121 ccgcgcgcgc gcgccacgcg tcggcgtgcg cgagggcctg gagcgcggcc gcggcggccg
      181 gggcgaggcg cgcgcccgtc gcctcgcggg cgcacgcctc cagctcgcgc cgcaaataat
      241 tccagggccc gccggccggc cccgcggccc ccagcccgtc gagcgcgagc acgttggtcg
      301 tgccctccca aatcgagagc acctgcgcgt cgcgcaggag cacgggcagc cccgtgtcct
      361 cgacgtagcc cgcgccgccg aaggcttcga gcacctcgct cgcgaccgcc accgcctgcc
      421 gcgccgtcgt caacttcatg aggggcgtca gcagccgcag cagccgcgac tgctcctcgg
      481 tcgcctcccc gcactcttcc agcccgagca gctccgcgac gcggaaggcg aggtggagcg
      541 cggcctcggc ctcggcctgc tgggcggcga gggtgtcggc gtgcagcggc ttctccgcga
      601 gcggcgcgcc gaaggcgacg cgcttgcgcg cgtagtcgcg ggcgagcgcc aggccgcggc
      661 gcatcagcga ggcggcgctc aagccgttcc acagccgcgt cacgttgagc atcggcgcga
      721 tgtggcgcac gccgtcggtc gtgccgcaga ccagctcggc cggggcgtcg cgcagcgtca
      781 gctccgcggt cggcaccttg cgcgtcccga gcttgtcctt gagccgatcg acttgaatgc
      841 cgcgcagccg gcccgcctcg tcccgcggct cgacgtagaa gagcgcgaga ccgcgcccgc
      901 ccgcggggtt gccttcgggc cgcgcgagcg tgagtgccgt ctcggcggtg gtggcagagg
      961 tgaaccactt gcgaccatag agccgccact ggccgccctc gtcgcggcgc gcggtcgtct
     1021 ccgtcaggcc cacgtcggag ccgcccgtca gctccgtcat ccactggccc gaagtccaga
     1081 actcgcgcgg ctcgcgcgcc gtcaggtgag gcaccgcgcg ttcgacgagc gcgcggttgc
     1141 ccgagtcgag cagcgtgcgc gccgcgccgt ccgtcatcgc cagcgggcaa ctgtagatgt
     1201 ccgtcgaggg ggtgaagagg tatgcgaggg cgcactggtg tatgcgcgag tagagaccgt
     1261 ggcggcgctc gtaggccgtg gcgaccacgc cgtgctccgc ggccagcgag gcggccacac
     1321 gccacagggg cgtcacctcg atgcggtcaa cgcgattgcc ccacgcgtcc cattgcgtga
     1381 gacgcggctc gttcagcctg tcttcgagtt gtagtcggta gaggtcgccg cccgcgagcc
     1441 gccccagctc cgcgagcgaa ggctctatct cgcgcagcat ctccgggggc agggcgcgcg
     1501 ttaagtacga gcgcaggacg cggtcgtctt cgtactggtt gccgagcgcg ggaggcgttt
     1561 gccggaatga catggccttc gtgtaagact ttataagagc ggtatctccc aggagccgcg
     1621 gacgtaggcg cagagcgcat cgccgtccca gccgcccatc cggaagcgca aaggccacgg
     1681 gcgcgtctcg tcgccgccga gggcgttggc gtggacatgc ccgttgacat cgatgtccca
     1741 cccttcgagc tgcaaggcga gcgtgtaggc gcggcggtcg atcaactcgg cgcgactcga
     1801 aatctccgac tccatgcgag actccttcga acgggatgag gcgatagagc tttgccgacc
     1861 cgagcatgtt ttgcgacaga ggacaggggc gccgccgcga gaaaccaacg cgccgcacta
     1921 tatcaacggc gtgcggcggc ggcaacgaaa tccttgaagc tcgtcgggac ttcgacaatc
     1981 gcgacagccg aagtcggggc cgcctgccgc gcggacacga ggtcgtgccc tctctccgaa
     2041 ggtcttgagc caaccgcgtg gtaaattacc gcggacaact catggcgtcc cgaagacacg
     2101 gagatcgaac atgaagcacg tcctgtcgaa gcacgtcctg ctcgccttcg tcctcgtcgt
     2161 cctttatcaa ggctcctctc tcgcttctga cagtgggcgc ggctccgcgc aagccgaggg
     2221 gcctgtgcga atcggcgtcg agccgcgcga cgtgttcgtc gagaggacgg cttcggcgca
     2281 gctcgtcagc ttcgacttcc tgcttgaaaa cctcggcgcg gagcctgcgc gcgtcgcgcg
     2341 gatagagctg tcggtctacg accggcgcgg ggcgctggtg ctgcgcaagt ttctcaccgc
     2401 cggcgggctg cgacacggcg actacgagat cgccaggctt gagccgaagg cgacggcgtt
     2461 cctgttcaac cctttcgaga atttctcgac ggaggtggag ctggcgagta tgcgctacga
     2521 gttcttcttc gaggccgccg aaggggcgag agagttctcc gcgtccgtca acatcgcgcc
     2581 gaaggtcttc aggccgaaga cgcggctccg gctcccgctc aaggggcgcg tgctcgtcca
     2641 cgacgggcac gacttctact cccatcaccg ccgcttcaac ctcgcacacc cgttcctgcg
     2701 cgagatcggt gtcacgcaca acttcacgcg cttcgcgtca gacctctgcg tcgtcaacga
     2761 gcggggcgac ctgcgccgcc agcgtagcga cgcgaacacg gactggtacg gcttcggcgc
     2821 gaccgtctac gcgcctggcg cgggccgcat cgtgcgcgca cgcggcgacg tgccggacaa
     2881 cgtcaacggc aaaagcacct tcaccatcga cgacttccgc aaagacgcga cggtgcccgc
     2941 cggcaacttc ctcgtcatcg accaccgcaa cggcgagtac agcctcatcg cacacctgaa
     3001 gcaggggagc ctcctcgtgc gcgaaggcga cacggtacgc gccggccagc ccatcggcca
     3061 gatgggcatc tccggcgacg cctacctccc gcacgtgcac tacgaactgc ggagcgcgag
     3121 caccgtcaac gcccacgcct tccccgccta cttccacaac ttcacacgcc tccacggctc
     3181 gcgccgcaca cacgtgctcc aggggcctgt ggattcgggc gacctccttg agagtcgttg
     3241 aagacgccgc cgtcaataac cgactgtcac ctgatgccga aaaaatatcc cgatcgagaa
     3301 ccgatgacga gccgcacctc agcgaccaca gcagacagat caacgatgaa tgaagccatc
     3361 ggaaacggca acacccccgc atgattgtta ctattcatcc atgaaacaga tgacgggcgt
     3421 caagagaaaa aaccacagac cctccctacc gtgccgccgg agcacttcca actcttcttc
     3481 acctgtaaag agcttgaatg cctcctacag tcgaaatcag tcccgggtgt ctggctgcct
     3541 catctccgag tcgcactccc gcggtgcgct cactcatcca tccgggcact tcgggcccat
     3601 gaggtgttat ccgcgtccgg agcagccttg gtttttcagc tactccgacg ccggtcgccc
     3661 aatcaggtgg tcaggcactt gaggcctatg aacagttcga caggcgctcg aaccactcgc
     3721 tgtagccgca ggcttcgatg ccgacgatct actcgccggt gaactgcgag tagaagctgc
     3781 gcacttcatc gagcgagtcg tgggacaggc gctgttcttg gatctcg
//
LOCUS       DBNGOH010000950         1500 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1061315, whole genome shotgun sequence.
ACCESSION   DBNGOH010000950 DBNGOH010000000
VERSION     DBNGOH010000950.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1500)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1500)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1500
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1061315"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..546
                     /locus_tag="AC159P_18505"
     CDS             <1..546
                     /locus_tag="AC159P_18505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697489.1"
                     /translation="EGARLSAAGLATEALRRYVNADAHPLLRERLAELYGAVVRERES
                     ATMRAYVAERKAVGRLTAEVSYLLIRPLLSGGVRAELRRFLWKVGEVGCLLDSVIDLR
                     SDERLGLLGFRPTLRDRLRLAAHTLVGGCGVLLRHPRLLGLFLEAAADDLLDRLAARA
                     APPSPDSPDTVTGARVCERTA"
     gene            complement(684..>1500)
                     /locus_tag="AC159P_18510"
     CDS             complement(684..>1500)
                     /locus_tag="AC159P_18510"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HIR3697490.1"
                     /translation="DVLVVQTLSQGTEAAKATLTELLVEEFAPRAVVERNDVRVRALE
                     GLPSAAGVLYGEPPAELEVVQHGVRFLVAPLAGQKTGAFLDQRENHLAARSYARGRAL
                     DCFTFNGGFALSIAPACESVTGLDISAEAVAAARRNAALNRASNVEFKESNVFDALRE
                     LESAGAVFDSIVLDPPAFAKNRASVAAAARGYKEINLRALKLLARGGHLVTCTCSYHM
                     TEPMFLEVVSAAAADARRRVQLVERRTQSRDHPVLAGVPETLYLKCIIARAIE"
BASE COUNT          203 a          524 c          541 g          232 t
ORIGIN      
        1 gagggcgcgc gcctctccgc cgcggggctg gccaccgagg cgctgcggcg ctacgtcaac
       61 gcggacgcgc acccgttgct gcgcgagcgg ctggcggagt tgtacggcgc ggtcgtgcgc
      121 gagcgcgagt ccgcgacgat gcgggcttac gtcgccgagc gcaaggcggt cgggcgactc
      181 acggcggagg tctcttacct gctcatccgc ccgctgctct ccggcggcgt gcgcgcggag
      241 ctgcgccgct tcttatggaa ggtgggcgag gtcggctgtc tgctcgacag cgtcatcgac
      301 ctgcgctcgg acgaacgact cggcctgctc ggcttccgcc ccacgctccg cgaccgcctg
      361 cgactcgccg cgcacacgct cgtcggaggc tgcggggtct tactccggca cccgcgcctg
      421 ctcggcctct tcctcgaagc cgccgccgac gacctgctcg accgcctcgc ggcacgcgcc
      481 gcgcccccct cccccgactc acccgacacc gtgaccggcg cccgcgtctg cgaacgcaca
      541 gcatgagagt aggcagtagg cggtaggcag taggcagcca agacaaacgc agggagaggc
      601 ttgggacttt acctcccaag cctcttcatc tttgttcttc cgtttttgct gcctactgcc
      661 tactgcctac tgcctactgc ttgctactcg atggcgcggg cgatgatgca cttgaggtag
      721 agggtttcgg ggactccggc gaggacgggg tggtcgcgcg actgtgtgcg ccgctcgacg
      781 agctggacgc ggcggcgggc gtccgccgcg gcggcggaga cgacttcgag gaacatcggc
      841 tcggtcatgt ggtaggagca ggtgcaggtg acgaggtggc cgccgcgcgc gagcagcttc
      901 agcgcgcgca ggttgatctc cttgtagccg cgcgccgccg ccgcgacgct ggcgcggttc
      961 ttggcgaatg cgggcgggtc gaggacgatg gagtcgaaca cggcgcccgc cgattcaagc
     1021 tcgcgcagcg cgtcgaagac gttggattct ttgaactcga cattcgaggc gcggttcagc
     1081 gcggcgttgc ggcgcgcggc ggcgaccgcc tccgccgaga tgtcgaggcc cgtcacgctc
     1141 tcgcacgcgg gcgcgatgct gagcgcgaag ccgccgttga aggtgaagca gtcgagcgcg
     1201 cggccccgcg cgtaggaacg cgcggcgagg tggttctcgc gctggtcgag gaaggcgccg
     1261 gtcttctgcc cggcgagcgg cgcgaccagg aagcggacgc cgtgctgcac gacctccagc
     1321 tccgcgggcg gctcgccgta gagcacgccc gccgccgacg gcaagccttc gagagcgcgc
     1381 acgcgcacgt cgttgcgctc gacgacggcg cgcggcgcga actcctcgac gagcagctcg
     1441 gtcagcgtcg ccttcgcggc ttccgtcccc tgcgagagcg tctgcacgac gagcacgtcc
//
LOCUS       DBNGOH010000951         3054 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1609797, whole genome shotgun sequence.
ACCESSION   DBNGOH010000951 DBNGOH010000000
VERSION     DBNGOH010000951.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3054)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3054)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3054
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1609797"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..957)
                     /locus_tag="AC159P_18515"
     CDS             complement(<1..957)
                     /locus_tag="AC159P_18515"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HIR3697491.1"
                     /translation="MLSAARHSTRPFALLAWLLCLPALGAAAAAQEVSQTPAPAESDE
                     VVRVTTELVQTDVMVFDKQGKFVDGLTADQFDLRVDGKPQAITFFERVKAGAVDEDAQ
                     LAAARGGATRREGDKSGAVLPLDRGRTVFFFIDDIHLSHGSLVRVQKTLLKFIEDELG
                     QNDEAAIISATGQPGFLQQLTGEKAVLRAAVERLKPRSFAMRDMQQPPMSESQALAID
                     RNDSSVTDYFVDALQRETPQMSRATLEQMVMMRARGILAQTNHYAVNTLASLEGVVRG
                     SAPLPGRKLVFFISDGFLLDTRDAAKDRLQRVTDAAARSGVVI"
     gene            1517..>3054
                     /locus_tag="AC159P_18520"
     CDS             1517..>3054
                     /locus_tag="AC159P_18520"
                     /inference="COORDINATES: protein motif:HMM:NF014214.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M36 family metallopeptidase"
                     /protein_id="HIR3697492.1"
                     /translation="MRKSLVFGLLVAALAAGSMFPLTRPGAARAPQPAAQDAPGARGE
                     GVGARRATLPNYDIRLEGRGEFRDIDVSPAGEEPNALQSADETARTRSAAVRNFRAGL
                     PPEHARNLRAEMNEAGALKNFFIDGGALSAPQSDVPDNIARNFLRREAALFALTERAV
                     NDLRLEKEDNDAGTTFLDYAQTVGGYKVFEGNVKVVVGKSGEVLSVREGFLVEGTRAR
                     FNAKLTEAQGIAKAFEYAGREVVPQFAEMRGRSWKGDSAEFANPLGSEYEHVLSELNV
                     VRVGKDALLAWHVYADVGPDEWYELLVDANSGELLLRHNLYVEQAQGTVYTEAPDKGA
                     RVLVSFVGDTVINTSFGWMGTSTVTTGNNVEAYLDRDANNAPDTTSGNGLSNGHAFAS
                     NQNFTFPFSTTVDPRTQQAAVVTNLFYYNNIMHDFSYNLGFTETARNFQTNNYGRGGT
                     GNDSVRAEAQDGSGTNNANFATPPDGQRPRMQQYLFTAPNPDRDSSMDGDVVFHEYGH
                     GISNR"
BASE COUNT          527 a         1028 c         1040 g          459 t
ORIGIN      
        1 gatgacgacg ccggagcgcg ccgccgcgtc cgtcacgcgc tggagacgat ccttggccgc
       61 gtcgcgcgtg tcgagcagga agccgtccga gatgaagaag acgagcttgc gaccgggcag
      121 cggcgccgag ccgcgcacga cgccttcgag cgaggcgagc gtgttgacgg cgtagtggtt
      181 ggtctgcgcg aggatgccgc gcgcgcgcat catgaccatc tgttcgagcg tcgcgcggct
      241 catctgcggc gtctcgcgct gcaaggcatc gacgaagtag tcggtgacgg agctgtcgtt
      301 gcggtcgatg gcgagcgcct gcgactcgga catcggcggc tgctgcatgt cgcgcatggc
      361 gaacgagcgc ggcttgaggc gctcgaccgc cgcgcgcagc acggccttct cgcccgtcag
      421 ttgttggagg aagccgggct ggcccgtggc cgagatgatg gccgcctcgt cgttctgccc
      481 cagctcgtct tcgatgaact tgagcagcgt cttctggacg cggacgaggc tgccatgcga
      541 gaggtggatg tcgtcgatga agaagaagac cgtgcgcccg cggtcgagcg ggagcacggc
      601 gcccgacttg tctccctcgc ggcgcgtcgc cccgccgcgc gccgccgcga gctgcgcgtc
      661 ctcgtccacg gcgccggcct tgacgcgctc gaagaaggtg atcgcctgcg gcttgccgtc
      721 cacgcgcagg tcgaactggt cggcggtcag cccgtccacg aacttgccct gcttgtcgaa
      781 gaccatcacg tccgtctgga cgagttcggt ggtgacgcgg acgacctcgt ccgactctgc
      841 gggcgcgggc gtttggctga cttcctgcgc ggcggcggcg gcgccgaggg ccggcaggca
      901 cagcagccac gcgagcagcg cgaagggccg tgtggagtgg cgtgcagcgg agagcataat
      961 tcggactcgc gaaagaggac ggacggacgg acgaatctta gcacgcgcga atcccgatgc
     1021 gggagcttga cggagagctg cccggacgcg gcgcgcagtt gtctcgggtt cgatgccgcg
     1081 gcggtcggat tcggatggcc gcgcggccat tctattctcg ggcgggcagg cgcggaatgc
     1141 gcaccggcgc cttaacatcc gcccgcgaat ttgctatcct ccagccctct tcgacaacga
     1201 cggcggcgct gccgccaatg ccccgacgat ccttacaatt gtaatccgcg gtgaggtcac
     1261 gcggctgaat gcctgtcagc cgcgaccgga gtgtgggcgg ggatgcttca cgtgccgcgt
     1321 gcgcttccgg cggtgtgacc gtggggcgga agtctgcgcg gcgaaccgtt cgccgtcgtg
     1381 aaaggacgat aaggcaatcc tcattcggcc aaagccgcag ggtgtccttc gatcaaagac
     1441 agggcgtctg caccttgggg aaaccaccgg gcgacccgtt gccttacctc aaccagcaac
     1501 ctacggaggg ataatgatgc gaaagagttt ggtcttcgga ttacttgtgg ccgctctggc
     1561 agccggctcg atgtttccct taaccaggcc gggcgcggcg cgcgcgccgc agcccgcggc
     1621 gcaggacgct cccggcgcgc gcggcgaggg cgtcggcgcg cggcgcgcga cgctgcccaa
     1681 ctacgacatc cgcctcgaag gccgcggcga gttcagggac attgacgtga gtcccgccgg
     1741 cgaggagccg aacgccctgc aaagcgccga cgagacggcg aggacgcgct ccgcggcggt
     1801 caggaacttc cgcgcgggcc tgccgcccga acacgcgcgg aacctccgcg ccgagatgaa
     1861 cgaagcgggc gcgctgaaaa acttcttcat cgacggcggg gcgctctccg cgccgcagtc
     1921 ggacgtgccg gacaacatcg cccgcaactt cctccggcgc gaggccgcgc tcttcgcgct
     1981 gaccgaacgc gcggtgaacg acctccggct cgaaaaggaa gacaacgacg cgggcaccac
     2041 cttcctcgac tacgcgcaga cggtcggcgg ctacaaggtc ttcgaaggta acgtgaaggt
     2101 cgtcgtcggc aagagcggcg aagtcctctc cgtccgcgag ggcttcctcg tcgagggcac
     2161 gcgcgcccgc ttcaatgcga agctcacgga agcgcagggc atcgccaagg ccttcgagta
     2221 cgcgggcagg gaagtcgtcc cgcagttcgc cgagatgcgc gggcgctcgt ggaagggcga
     2281 cagcgcggag ttcgccaacc cgctcggctc cgagtacgag cacgtgctct cggagctgaa
     2341 cgtcgtgcgc gtcggcaaag acgcgctcct cgcgtggcac gtctacgcgg atgtcggccc
     2401 cgacgagtgg tacgagctgc tcgttgacgc gaacagcggc gagctgctct tgcgccacaa
     2461 cctctacgtc gagcaggcgc agggcacggt ctacacggag gcgccggaca agggcgcgcg
     2521 cgtgctcgtc tccttcgtcg gcgacaccgt catcaacacg agcttcggct ggatgggcac
     2581 ctcgaccgtg acgaccggca acaacgtcga agcctacctc gaccgcgatg ccaacaacgc
     2641 gccggacact accagcggaa acggcctctc gaacggtcac gccttcgcct cgaaccagaa
     2701 cttcaccttc cccttctcga ccaccgttga cccgcgcacg cagcaggcgg cggtcgtgac
     2761 gaatcttttc tattacaaca acatcatgca cgacttctcc tacaacctcg ggttcacgga
     2821 gacggcgcgc aacttccaga ccaacaatta cgggcgcggc ggcacgggca acgactcggt
     2881 gcgggccgaa gcgcaggacg gctcgggcac gaacaacgcc aacttcgcca cgccgcccga
     2941 cggccagcgc ccgcgcatgc agcagtatct tttcaccgcg cccaaccccg accgcgacag
     3001 ctcgatggac ggcgacgtgg tcttccacga gtacggccac ggcatctcga accg
//
LOCUS       DBNGOH010000952         4191 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4358555, whole genome shotgun sequence.
ACCESSION   DBNGOH010000952 DBNGOH010000000
VERSION     DBNGOH010000952.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4191)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4191)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4191
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4358555"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..975
                     /locus_tag="AC159P_18525"
     CDS             <1..975
                     /locus_tag="AC159P_18525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR3697493.1"
                     /translation="GGDSASADQDQTLNQLLVEMDGFDAISGVVVIASTNRPDILDQA
                     LTRPGRFDREITVNLADMRGRERILQVHARKLKLEEGLDLGWIARGTPGFSGADLANL
                     LNEAAIAATRDGSAAVGRPHVEYARDKILMGVERLGFMMDEEERYATAVHEAGHVAVG
                     LAVKDGDPVHKVSILPRGRALGVTQALPERDRLMKKRGYLEDQIAMLLGGRAAEMLLL
                     DTMTAGASNDIERAVEIARKMVAEFGMSPLGPIHLGKPEDPHSQTILDRIEQATGDII
                     GEQLKRACEVVDANRAEIARLVEGLMERDTLDAEEIHECFGLNADRAA"
     gene            1233..1964
                     /locus_tag="AC159P_18530"
     CDS             1233..1964
                     /locus_tag="AC159P_18530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697494.1"
                     /translation="MPYPSVERIELPVLQELVATGGMEDVRFLYDRLASYFPQLGDEE
                     VLAHSNGHLRQWRRLVQRAGRDLTEKKQLERVRGRWTITNQGRRRVADEDTHFSLTES
                     SPAETAGSGVFTHADIQQMLVEIGRVLGHHAEVEYDYYDVVWRADEGSPRLSHVFEVQ
                     RKGNVDAALAKLKRAYDTQRSKPFLVVASERDTNRAERQLSAARTGPFHEIGRVATIL
                     SFEQLRRLHRALTSVEDILGQIFER"
     gene            1961..>4191
                     /gene="mutS"
                     /locus_tag="AC159P_18535"
     CDS             1961..>4191
                     /gene="mutS"
                     /locus_tag="AC159P_18535"
                     /inference="COORDINATES: protein motif:HMM:TIGR01070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HIR3697495.1"
                     /translation="MTTDTTPLRQQYLDIKRRHPEALLLFRIGDFYEAFDDDARLLAR
                     ELDIVLTSKPMGRNVRVPLAGIPHHSLERHLGTLISKGHRVAICEQISASPVKGESAG
                     RGLIERDVVRVVTPGTVLEPGLLQHKVNNYLAALFVEGKRAGIAYADVTTGDFATTEV
                     EAAGALDELHRIGPSELLIPESFAAVERELPGFLTRLDDSTFEAARAKRTVFRHFGTR
                     TLAPLGLTGWPLAVSAAAAIITYLGETQGAAAEHLTRLTSYKTHAYMTLDAQTVRSLE
                     IFEGAGGTPSLLSTIDETRTAMGGRLLRRWLRQPLLDVAEITRRQEHVAWFVAHDKDR
                     AEVAAALESVHDLERLSSRARAGLASPWDVLALKQSLETIPRLRAVLQRDAKRFGSAL
                     AALPECAAVSALIGSAVNEELPRRAEGVGVIRDGFSEELDSLRKLLREGKTFLADMER
                     RERARTGIKSLRVGYNKVFGYFIEVTRPNLRLVPGDYTRKQTLAQSERFVTLELKEHE
                     SLVLNAQDRIAELEASLFRRVCTEIGKARENILAAAGTIAHLDVIVSFARSAADWNYA
                     RPVVADDAVLVIKDGRHAVLERTLGRDAFVPNDTRLGGGDAPDVALITGPNMSGKSTY
                     LRHAALVVVLAQAGSFVPARAASVGLTDRIFTRIGLYDRIGSGESTFMTEMVETAHIL
                     HHATPRSLILLDELGRGTSTYDGLAVARSVLEYIHNHPRLRSKTLFATHYHELTELAE
                     VLPR"
BASE COUNT          716 a         1560 c         1295 g          620 t
ORIGIN      
        1 ggcggcgact cggcctccgc cgaccaggat cagacgctca accagctcct cgtcgagatg
       61 gacggcttcg acgccatcag cggcgtcgtc gtcatcgcct cgaccaaccg gcccgacatc
      121 ctcgaccagg cgctcacgcg ccccggtcgc ttcgaccgcg aaatcaccgt caacctcgcc
      181 gacatgcgcg gacgcgagcg catccttcag gtgcacgcgc gcaagctcaa actcgaagag
      241 ggcctcgacc tcggctggat cgcgcgcggc acgcccggct tctccggcgc ggacttggcg
      301 aacctgctga acgaggcggc gattgccgcc acgcgcgacg gctccgcggc ggtcggtcgc
      361 ccgcacgtcg agtacgcgcg cgacaagata ctgatgggcg tcgagcgcct cggcttcatg
      421 atggacgagg aggagcgcta tgccaccgcc gtccacgaag cgggccacgt cgccgtcggc
      481 ctcgccgtca aagacggcga ccccgtccac aaggtctcca tcctgccgcg cgggcgcgcg
      541 ctcggcgtga cgcaggcgct gcccgaacgc gaccgcctca tgaagaagcg cggctacctc
      601 gaagaccaga tcgcgatgct cctcggcggt cgcgccgccg agatgctcct gctcgacacg
      661 atgacggcgg gcgcctcgaa cgacatcgag cgcgccgtcg agatcgcgcg caagatggtc
      721 gccgagttcg gcatgagtcc gctcggcccc atccacctcg gcaagcccga agacccgcac
      781 agccagacca tcctcgaccg catcgagcag gccacgggcg acatcatcgg cgaacagctc
      841 aagcgcgcct gcgaagtcgt cgatgcgaac cgggccgaga tcgcgcggct cgtcgaaggc
      901 ttgatggagc gcgacacgct cgacgccgaa gagattcacg agtgcttcgg cctcaacgcc
      961 gacagggccg cctgagccgt acgacagaca agtaaatcgc acggaaagcg gcctgcggct
     1021 cgaaaaagcc gcgggccgct tttttgataa gtgttgccac ccctatcttt atcttgaact
     1081 gcaacccctc ttgcagcgat tcccgcacgg ggccgtgtcg ttgcttgatt gcgggcgcgt
     1141 ttatcctgta acttggcaca cgtcaccgag gccgtttttt cgcgcggaga tttcgcctcc
     1201 cgcagccctt ctactcccga gcgtaaccgc ggatgccata ccccagcgtc gagcgcatcg
     1261 aactgcctgt gcttcaggag ttggtcgcca ccggcggcat ggaggacgtg cgctttctct
     1321 acgaccggct cgcctcctac ttcccgcagc tcggcgacga ggaggtgctc gcgcactcga
     1381 acgggcacct gcggcagtgg cggcggctcg tgcagcgcgc gggccgcgac ctcacggaga
     1441 agaagcagct tgagcgcgtg cgcgggcgct ggacgataac caatcagggg cgacgccgcg
     1501 tcgcggacga ggatacgcac ttctcgctga ccgaaagctc gcccgccgag accgccggct
     1561 ccggcgtctt cacgcacgcc gacatacagc aaatgctggt cgagatcggt cgcgtgctcg
     1621 ggcaccacgc ggaggtcgag tacgattatt acgatgtcgt ctggcgcgcg gacgagggca
     1681 gcccgcgcct gagccacgtc ttcgaggtgc agcgcaaggg gaacgtggac gcggcgctcg
     1741 ccaagctgaa acgcgcgtac gacacgcagc gctcgaagcc cttcctcgtc gttgccagcg
     1801 agcgcgacac gaaccgcgcc gagcggcagc tcagcgcggc gcgcaccggc cccttccacg
     1861 agatcgggcg cgtcgccacc atcctcagtt tcgagcagct ccgacgcctt caccgcgccc
     1921 tcacctcggt cgaagacatc ctcggtcaaa tcttcgaacg atgaccaccg acaccacccc
     1981 gctccggcag cagtacctcg acatcaagcg ccgccacccc gaagcgctgc tgctcttccg
     2041 catcggcgac ttctacgaag ccttcgacga cgacgcgcgc ctgctcgcgc gcgagctgga
     2101 catcgtcctc acctcgaagc cgatgggccg caacgtccgc gtcccgctcg cgggcatccc
     2161 gcaccacagc ctcgaaagac atttggggac gctcatctcg aaaggccacc gcgtcgccat
     2221 ctgcgagcag atttccgcgt cgccggtcaa gggcgagtct gcgggccgcg ggctgatcga
     2281 gcgcgacgtg gtgcgcgtcg tcacgcccgg cacggtgctc gaacccggcc tgctccagca
     2341 caaggtcaac aactacctcg ccgcgctctt cgtcgaaggc aagcgcgcag ggatagctta
     2401 cgccgacgtg accactggcg acttcgcgac caccgaagtc gaagccgcgg gcgcgctcga
     2461 cgagcttcac cgcatcggcc catccgaact gctcatcccc gaaagcttcg ccgccgtcga
     2521 gcgcgagcta cccggcttcc tcacgcgcct cgacgattcg accttcgagg cggcgcgcgc
     2581 gaagcgcacc gtctttcggc acttcggcac gcgcacgctc gcgccgctcg gcctcacggg
     2641 ctggccgctc gccgtctccg ccgccgcggc gatcatcacc tacctcggcg agacgcaggg
     2701 cgccgccgcc gaacatctca cgcgcctgac gagctacaag acgcacgcct acatgacgct
     2761 cgacgcacag accgtccgca gcctcgaaat cttcgagggc gcgggcggca ccccttcgct
     2821 gctctcgact atcgacgaga cgcgcacggc gatgggcggt cggctgctgc gccgctggct
     2881 gcgtcaaccg ctgctcgacg tggcagagat cacgcggcgg caggagcacg tcgcgtggtt
     2941 cgtcgcccac gacaaagacc gcgcggaggt ggccgccgcg ctcgaatccg tccacgacct
     3001 tgagcgtctg tcttcgcgcg cgcgcgcggg actcgcctcg ccgtgggacg tgctcgcctt
     3061 gaagcagagc ctcgaaacca tcccgcgcct gcgcgccgtc ttgcagcgcg acgccaagcg
     3121 cttcggctcg gcgctcgcgg cgctgcccga atgcgccgcg gtctcggcgc tcatcggctc
     3181 ggcggtcaac gaagagctgc cgcgccgcgc cgaaggcgtc ggtgtcatcc gcgacggttt
     3241 ctccgaagag cttgattcgc tcaggaagct gctgcgggaa gggaagactt tcctcgccga
     3301 catggagcgg cgcgagcgcg cgcgcaccgg gatcaaaagt ctgcgcgtcg gctacaacaa
     3361 ggtcttcggc tacttcatcg aggtgacgcg cccgaacctg cggctcgtgc ccggcgacta
     3421 cacgcgcaag cagacgctcg cgcagtccga gcgcttcgtc acgctcgaac tcaaggagca
     3481 cgaatcgctc gtccttaacg cgcaggatcg catcgccgaa ctcgaagcaa gcctcttccg
     3541 ccgcgtctgc acagagatcg gcaaggcgcg cgagaacatc ctcgccgcgg cgggcaccat
     3601 cgcgcacctc gacgttatcg tctcctttgc gcgcagcgcc gccgactgga attacgcgcg
     3661 gcccgtcgtc gccgacgacg ccgtgctcgt catcaaagac ggtcgccacg ccgtgctcga
     3721 acgcacgctc gggcgcgacg cgttcgtccc gaacgacacg cggctcggcg gcggcgacgc
     3781 gccggacgtg gcgctcatca ccggcccgaa catgtccggc aagtcaacct atctgcgcca
     3841 cgccgcgctc gtcgtcgtcc tcgcgcaggc cggcagcttc gtgcccgcgc gcgcggccag
     3901 cgtcggcctc accgaccgca tcttcacgcg catcggcctc tacgaccgca tcggcagcgg
     3961 cgaatcgacc ttcatgaccg agatggtcga gaccgcccac atccttcacc acgcgacgcc
     4021 gcgctcattg atcctgctcg acgagctggg gcgcggcacc tccacctacg acggactcgc
     4081 cgtcgcgcgc tccgtcctcg aatatatcca caaccacccg cgcctgcgct cgaagacgct
     4141 cttcgccacg cactaccacg agctgaccga gctcgcggaa gtcctgccgc g
//
LOCUS       DBNGOH010000953         2445 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_327789, whole genome shotgun sequence.
ACCESSION   DBNGOH010000953 DBNGOH010000000
VERSION     DBNGOH010000953.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2445)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2445)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2445
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_327789"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            127..1284
                     /locus_tag="AC159P_18540"
     CDS             127..1284
                     /locus_tag="AC159P_18540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006386886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HIR3697496.1"
                     /translation="MKINVIGGGPAGLYFALLLKKRGGAAHEIEVFERNAPGDTFGWG
                     VVFSGRTLSNLREADAESHRRVTESFETWDNVDVVHRGEKVSIRGNHFSGVARIRLLN
                     ILQERCEELGVRLRFRTNVADVDALRRDCDLLVGADGVNSEVRQRYADAFRPTLGAGR
                     NKYIWYGTRRLFHGLTLTFRESEVGVFSAHSYKFSRTHSTFIVECDEETWARAGLGRM
                     PDAEARGYLAGVFGRDLEGEELLSNNSRWINFLLVKNERWRHENVVLLGDALHTAHFS
                     IGSGTKLALEDAIALFDCFSHTRDVPSALIEFERTRKPVIEQYQQAAHESRLWFENVR
                     DLLHLAPLPFAYNLMTRSGKVDFESVRRRDPQFAAAYEEQRGRGGDGETGR"
     gene            complement(1343..>2445)
                     /locus_tag="AC159P_18545"
     CDS             complement(1343..>2445)
                     /locus_tag="AC159P_18545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HIR3697497.1"
                     /translation="QIIDFLSVKRRPEKEKYFERFLKLDGRHRRALAEATGGESWRWR
                     DETEVRAFLKDAPDELVERVLDERELEALRFGYRERVTDTHLAHVKELPEVLPGIFAA
                     AAVRAREAGFDGVELHYAHAYTRASFLSRRNDRADGYGGSPAARVRLPLEVYAEVRRR
                     VGHDYVVGARFLGDEVIEGGSRVRDAAYYGVEFARAGLDFISVSKGGKFEDAKQPKVG
                     HAVYPYTGQSGYECMPTVISDARGPFGRSLPLVREIKRAVNVAGFRTPVVGTGGVGTF
                     ELAEEILRSGAADIVGFARQALADPDWFLKVRLGRGAEVRRCTYTNYCEALDQMHKQV
                     TCKLWDRVGMDEPGLALSSDGRRRLLPPEWEQ"
BASE COUNT          398 a          834 c          820 g          393 t
ORIGIN      
        1 gacggtcttg agcaaagagg tgcacgaagt ggaccgcgac accctccggc gcgccgcggc
       61 ggaagtgctc ggcgggaatg atgcggcggg cgcgggcacg acggcttaaa caagttctcc
      121 cgacggatga agatcaacgt catcggcggc gggccggcgg ggctgtactt cgcgctcctg
      181 ttgaagaagc gcggcggggc ggcgcacgag atcgaggtgt tcgagcggaa cgcgccggga
      241 gacacgttcg ggtggggcgt ggtcttttcg ggccggacgc tctcgaacct acgcgaggcg
      301 gacgccgagt cgcaccggcg cgtcaccgaa agtttcgaga cgtgggacaa cgtggatgtc
      361 gtccaccgcg gcgagaaggt ctcgatccgc ggcaaccact tctcgggcgt cgcgcgcatc
      421 cgcctgctga acatcctgca agagcgctgc gaggagctgg gcgtccggct gcgcttccgc
      481 acgaacgtcg cggacgtgga cgcgctacgg cgcgactgcg acctcttggt cggggccgac
      541 ggcgtcaaca gcgaagtgcg gcagcgctac gcggacgcct tccggcccac cctgggcgcg
      601 ggccgcaaca agtacatctg gtacggcacg cgccggctct tccacggcct gacgctcacc
      661 ttccgcgagt cggaggtggg cgtcttttcc gcgcactcct acaagttcag ccggacgcac
      721 agcaccttca tcgtcgagtg cgacgaagag acgtgggcgc gcgcggggct cggccgaatg
      781 ccggacgcgg aggcgcgcgg ctacctcgcc ggcgtcttcg ggcgcgacct cgaaggcgag
      841 gagcttttgt cgaacaactc gcgctggatc aacttcctgc tcgtgaagaa cgagcgctgg
      901 cgtcacgaga acgtcgtgct cctcggcgac gcgctgcaca ccgcgcactt ctccatcgga
      961 tcgggcacca agctcgcgct cgaagacgcc atcgccctct tcgactgttt ctcgcacacg
     1021 cgcgacgtgc cctccgcgct cattgagttc gagcgcacgc gcaaacccgt catcgaacag
     1081 taccaacagg ccgcgcacga aagccgcctc tggttcgaga acgtcagaga ccttctccat
     1141 ctcgcgccac tgcccttcgc ctataacctc atgacgcgca gcggcaaggt cgatttcgag
     1201 agcgtgcggc gccgcgaccc gcagttcgcg gcagcttacg aagaacagag ggggagagga
     1261 ggagacgggg agacggggag atagagagca gcgccattaa gcatgctcct tatctccccg
     1321 tctccccgtc tccccctctt ccttactgtt cccactccgg cggcaataac cgacgccgcc
     1381 cgtcgctgct gagtgcgagg ccgggttcgt ccattccaac ccgatcccag agcttgcacg
     1441 tgacctgctt gtgcatctgg tcgagcgcct cgcagtagtt ggtgtaggta cagcggcgca
     1501 cctccgcgcc ccgcccgagt ctgactttca agaaccagtc ggggtctgcg agcgcctgtc
     1561 gcgcgaagcc gacgatgtcc gcggcgccgc ttcttaaaat ctcttcggcc agctcgaagg
     1621 tgccgacgcc gccggtgccg acgacgggcg tccgaaaacc cgcgacgttg acggcccgct
     1681 tgatttcgcg caccagcggg aggctgcgac cgaaggggcc gcgcgcgtcg gagatgacgg
     1741 tcggcatgca ctcgtagccg ctctgacccg tgtaggggta gacggcgtgg ccgaccttcg
     1801 gctgtttggc gtcctcgaac ttgccgccct tcgagacgga gatgaagtcc agccccgcgc
     1861 gcgcgaactc gacgccgtag taggccgcgt cccggacgcg gctcccgccc tcgatgacct
     1921 cgtcgccgag gaagcgcgcg ccgacgacgt aatcatgccc gacgcgccgg cgcacctccg
     1981 cgtagacttc gagcggcagg cgcacgcgcg ctgcgggcga gccgccgtag ccgtcggcgc
     2041 ggtcgttgcg gcggctgagg aaggaggccc tcgtgtaggc gtgcgcgtag tgcagctcga
     2101 cgccgtcgaa gcccgcctcg cgcgcgcgca ccgccgccgc ggcgaagatg ccggggagca
     2161 cttcgggcag ctccttgacg tgcgcgaggt gcgtgtccgt cacgcgctcg cggtagccga
     2221 agcgcaaggc ttcaagctcg cgctcgtcga gcacgcgctc gaccaactcg tccggcgcgt
     2281 ccttcaggaa cgcgcgcact tccgtctcgt cgcgccagcg ccagctttcg cccccggtcg
     2341 cttcggcgag cgcgcgccgg tgtctcccgt ccagcttcag gaagcgctcg aaatattttt
     2401 ccttttcggg ccggcgcttg acggagagga agtcgatgat ctgta
//
LOCUS       DBNGOH010000954         3760 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2343393, whole genome shotgun sequence.
ACCESSION   DBNGOH010000954 DBNGOH010000000
VERSION     DBNGOH010000954.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3760)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3760)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3760
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2343393"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            316..1470
                     /locus_tag="AC159P_18550"
     CDS             316..1470
                     /locus_tag="AC159P_18550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011396634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR3697498.1"
                     /translation="MNVSFVEASASLRRAVSDAARGLVERESLVELVALAAVAQEHVL
                     VIGPPGTAKSEAVRRVARATGGLYFEYLLGRFTEPSEIFGPVDLRRLKEGVVETETSG
                     MLPEAEVAFLDEIFQGSTAILNTLLGVLNERVFRRGHTRLTCPLRVCVGASNALPEDA
                     ALAAFADRFLIRAFVEPVPDPMLERLLEGGWSLGQEAAGRAASIGDVDTLAEAARVAD
                     MSGVRPVLGKAIRILRGAGITLTDRRAVKAQRLIAAAAVLAGRERPTEADLWPVVFVV
                     PTHEQQALARDCLRELLSSTENGALPAAAEEASRGPLARAARIAQAAREILGERPAAE
                     DPSASKSWRLKLEGVAREIDAGFTPEQMPQELVEVRVHIIDLLGQTNGRQ"
     gene            1478..1915
                     /locus_tag="AC159P_18555"
     CDS             1478..1915
                     /locus_tag="AC159P_18555"
                     /inference="COORDINATES: protein motif:HMM:NF040397.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bpX5 domain-containing protein"
                     /protein_id="HIR3697499.1"
                     /translation="MRDNRLIEVEWRPRRQPLAPIGAAARGAAAARLALRLLDEDDDS
                     LAELKGVGGRGVLVILGAAERLPWVEGITYLGRDEQTPALLMPTTLAPSAPAPLLTRA
                     FATRFPRLLPCAVLTGPAALVPVAGARAVAREALLKWLGRAAS"
     gene            1912..3723
                     /locus_tag="AC159P_18560"
     CDS             1912..3723
                     /locus_tag="AC159P_18560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697500.1"
                     /translation="MKLPAALSHWAQYLDIFPEEVSVALAPIVQRVSLLVGPLGPRFR
                     GAEGEPDGFGGLDRRGHYERLLLSEWALADEFQDEFTRRAVMGEHLFLNPARNVRAGA
                     RVSLALFDAGPSQLGAPRIAHLAALVALARRAEAAGATFGWGVLQQTETRTFDDVTAS
                     GVRRLLESRSHHEATDEEVEKWLSPSASWSGAEDVWLVGGRRLARLCVGRAISRLSVE
                     DPLEPDSRRLILSARGATAAPKEVALDLPPDRVGTRLLRDPFEAAVAEVRKVEGEIAA
                     ASNLLFDRGGTRLFVRSQAWGVTAFHVPNSPRAGAGKPRHYRTRRWKRVAAVGKVGRA
                     VALVSAEETLVRLEYCRQGDTKLPAGNYAGYNQGIFFNAPDGDDAPLAPCLDSPWNKE
                     LAVLDAAGTLFRLVKLSGAEKSIGGKPVVGTVQVVATRVLAAALVKSRLVYVGMEWPG
                     ESLHVVSIGTDIARTPIPFHEGRATKAFFGPPADVGHEKFGLLAVELGEFQWAVMSAK
                     GEDLLARPHGASVVGVLAGGPREPGLLALEDDGRTLRLHGRNWRQKVLESSTPIERVA
                     VSADAPRVAYSTFGGEVVVYSLQHRAELCRYQQGVRP"
BASE COUNT          527 a         1306 c         1357 g          570 t
ORIGIN      
        1 cccggaggtt cttgaacagc ttactgccgg tcaggcggca gtgatgaacg gatgtaggcg
       61 agcctttcga agagaccttt acgccctgag taacgtcgcc cgatgagggg cgacagtcgg
      121 acgctccacg ggtttggaaa ccgattgcaa taccgacgcc cttcccataa tatcgatcag
      181 ggcgctccga tcatgccgcc cgcgggccgc ggatgtcgcg tccgccctca ggttcggaaa
      241 caagccctta cctgctccgc gcaaaccact cccgttcgag tccatttcgc cggctttatc
      301 atccacgtca tgagtatgaa tgtttctttc gttgaagcgt cggcctcgtt gcgccgcgcc
      361 gtctccgatg ccgcgcgcgg cctggtcgag cgcgagtctc tggtcgagct ggtggcgctc
      421 gccgccgtgg cgcaggagca cgtgctcgtc atcggcccgc cgggcacggc caagagcgag
      481 gccgtgcgcc gcgtggcgcg ggccacgggc ggcctgtact tcgaatacct gctcggtcgg
      541 ttcaccgagc cgagcgagat cttcggcccc gtggacttgc gcaggctcaa ggagggggtc
      601 gtcgagacgg aaacctccgg catgctgccc gaagccgagg tcgccttcct cgacgaaatc
      661 ttccagggat cgacggcgat cctcaacacg ctgctcggcg tgctgaacga gcgcgtcttc
      721 cggcgcggcc acacgcgcct gacgtgcccg ctgcgcgtgt gcgtgggggc gtcgaacgcg
      781 ctccccgaag acgccgcgct ggccgccttc gccgaccgct ttctgatccg cgccttcgtc
      841 gagccggtgc ccgaccccat gctcgaacgc ctgctcgaag gcggatggtc gctcggccag
      901 gaggccgcgg ggcgcgcggc cagcatcggc gacgtagaca cgctggccga agcggcgcgc
      961 gtggccgaca tgtcgggcgt gcgccccgtg ctcggcaagg ccatccgcat cctgcgcggg
     1021 gcgggcatca cgctgaccga ccggcgcgcc gtgaaggccc aaaggctgat cgccgccgcg
     1081 gcggtgctgg cgggccgcga gaggccgacc gaggccgacc tctggccggt cgtcttcgtc
     1141 gtcccgacgc acgaacagca ggcgctggcg cgcgactgtc tgcgcgagct gctctcgtcc
     1201 acggagaacg gggcgctgcc ggcggcggcg gaagaagcct cgcgcgggcc gctcgcgcgc
     1261 gccgcgagga tcgcgcaggc ggcgcgcgag attctgggcg agcgccccgc ggcagaagac
     1321 ccttcggcgt cgaagtcatg gcggctgaag ctcgaaggcg tcgccagaga gatcgacgcg
     1381 ggcttcacgc ccgagcagat gccgcaggag ttggtcgagg tgcgcgtcca catcatcgat
     1441 ctgctcggcc agaccaacgg gcggcagtag cagcctcatg cgcgacaacc gactcatcga
     1501 ggttgagtgg cgtccgcgcc ggcagcctct cgcgccgatc ggcgccgcgg cgcgcggagc
     1561 ggctgccgcg cgccttgccc ttcgcctgct ggacgaggac gacgactcgc tcgcggagtt
     1621 gaagggcgtc ggcggtcgcg gcgtgctcgt catcctcggc gcggcggaga ggctgccgtg
     1681 ggtcgaaggg atcacgtacc tcgggcgcga cgagcagacg cccgcgctgc tcatgccgac
     1741 cacgctcgcg ccttccgccc cggctccgct cctgacacgc gcgttcgcca cgcgcttccc
     1801 aagactcctg ccgtgcgccg tgctcacggg gccggcggcg ctcgtccccg tcgccggggc
     1861 gcgggcggtc gcccgcgagg ctctgctcaa gtggttgggg agggccgcat catgaaactc
     1921 cccgccgcgc tctcgcactg ggcgcagtac ctcgatatct tccccgaaga ggtgagcgtc
     1981 gcgctcgcgc ccatcgtcca gcgcgtctct ttgctggtcg gcccgctcgg cccgcgcttc
     2041 cgcggcgcgg agggcgagcc tgacgggttc ggcggtctcg accggcgcgg ccactacgag
     2101 cgcctcctgc tctcggagtg ggcgctcgcc gacgaattcc aagacgagtt cacgcggcgc
     2161 gccgtgatgg gcgagcacct gtttttgaac cccgcgcgta acgtgcgggc cggggcgcgc
     2221 gtctcgctcg cgctgttcga cgccggcccg agccaactcg gggcgccgcg catcgcgcac
     2281 ctggccgcgc tcgtcgccct cgcgcgcagg gccgaagccg ccggggcgac gttcggctgg
     2341 ggcgtgcttc agcagacgga gacgcggacg ttcgacgacg tgacggcgtc cggcgtccgg
     2401 cgcctgctcg aatcccgcag ccatcacgag gcgacggatg aagaggtcga gaagtggctg
     2461 tcgccaagcg cctcgtggag cggcgcggag gacgtgtggc tcgtcggcgg caggcggctc
     2521 gcgcgcctct gcgtgggcag agcgatctca cgactctcgg tcgaagaccc gcttgagccg
     2581 gattcgcggc gactgattct gtcggcccgc ggcgcgacag ccgccccgaa ggaggtcgcg
     2641 ctcgaccttc cgcccgaccg ggtcggcacg cgccttttgc gcgacccgtt cgaggccgcg
     2701 gtcgcggagg tgcggaaggt cgaaggcgag atcgccgccg cctcgaacct gctcttcgac
     2761 aggggaggga cgaggctgtt cgtgcgctcg caggcgtggg gcgtcacggc cttccacgtg
     2821 ccgaactcgc cgcgcgcagg ggcggggaag ccgaggcact accgcacgcg tcgttggaag
     2881 agggtcgcgg cggtcggaaa ggtcggtcgc gccgtggccc tcgtctcggc ggaagaaact
     2941 ctggtgcggc tggagtattg ccgccagggc gatacgaaac ttcccgccgg caactacgcc
     3001 ggctacaacc aggggatatt cttcaacgcg cccgacggcg acgacgcgcc gctcgcaccg
     3061 tgcctggact cgccgtggaa taaggagctg gccgtcctcg acgcggcggg cacgctcttc
     3121 cgtctggtca agctgagcgg cgcggaaaag agcatcggcg ggaagcctgt ggtcgggacg
     3181 gtgcaggtcg tcgccacccg cgtgctcgcc gccgcgctcg tcaaatcgcg cctcgtctac
     3241 gtcggaatgg agtggccggg cgagagcctc cacgtcgtct ccatcggcac ggacatcgcg
     3301 cgcacgccga tccctttcca cgagggccgg gccaccaaag cgttcttcgg cccgccagcc
     3361 gatgtcggcc acgagaagtt cgggctgctc gccgtcgagc tgggcgagtt tcagtgggcc
     3421 gtgatgagcg cgaagggcga agaccttctg gcacgaccgc acggggcgag cgtggtgggc
     3481 gtgctcgccg gcggcccccg cgagccgggg ctgctcgcgc tcgaagacga cgggcgcacg
     3541 ttgaggctgc acgggcgcaa ctggcggcag aaggtgttgg agtcgagcac ccccatcgag
     3601 cgcgtggccg tgagcgcgga cgccccgcgc gtcgcctact cgacgttcgg cggcgaggtc
     3661 gtcgtctact cgcttcagca ccgcgccgaa ttgtgtcgct accagcaggg ggtgaggccg
     3721 tgaccggtgt gctgcctcgc aggcttgtcc accgcggctg
//
LOCUS       DBNGOH010000955         3377 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1610570, whole genome shotgun sequence.
ACCESSION   DBNGOH010000955 DBNGOH010000000
VERSION     DBNGOH010000955.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3377)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3377)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3377
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1610570"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            15..1028
                     /locus_tag="AC159P_18565"
     CDS             15..1028
                     /locus_tag="AC159P_18565"
                     /inference="COORDINATES: protein motif:HMM:NF024821.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3697501.1"
                     /translation="MRKRNLLSLVVGAALLVGGAHLSNVASANDGGNPAAGAAVEAPQ
                     KKKGGGFASALGAPFRAIGRLFGGGKKEKKEQPVAEKKDGQKAENREDAKPRRLTEKD
                     AADFQSATMMRVNYRESAAVAPAAVAAPHALVAEARALLARDEFGEAISRLSSAASAA
                     PNLAEAHHLLGYAYDRKGLHQLSLDAYRRAVKLAPNDAQLLNDYGYTLYLRGQHKEAK
                     ELLKRAAKRAPGDERVWNNLAVVQVKLGSYDDAFKSFARAGGEFKGRMNVANALERAG
                     RYEDALKHYEGARKVNPASRAALQHLADLYQRLGRKEEEEAARQLLAAPKQEKPKSEN
                     GGQ"
     gene            1136..1963
                     /locus_tag="AC159P_18570"
     CDS             1136..1963
                     /locus_tag="AC159P_18570"
                     /inference="COORDINATES: protein motif:HMM:NF013618.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008933 - peptidoglycan lytic
                     transglycosylase activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HIR3697502.1"
                     /translation="MFFRLALACCALTLLPAAALADRLHLKNGAVIEVDDAWENADGV
                     WYRRAGVVQLIAKDRVSRIEKSSGEKAADVATPVAPAEVVKRGAAAAPRPAQTPAART
                     VWIHLVGGAKIEVEEADETSEGVWYKRASFSIFLDKERVARIERDAPTPAAAAAGAPA
                     SAHRAFGWTTGSTRLDELIRRNGARHGVDPYLIFCVMEHESHFRSRAVSHAGARGLMQ
                     LMPGTARRFGVRDVFDPAQNVAGGTRYLKELLDMWGGRVDLALASYNAGEGAVMKYG"
     gene            2114..2902
                     /locus_tag="AC159P_18575"
     CDS             2114..2902
                     /locus_tag="AC159P_18575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697503.1"
                     /translation="MPRLLVYAVLSAAVLLQGAVASRAQAQRADEQVLTNASVIKLVR
                     GGFSDKAVIAIINSRPTRFDLATERLIELKKSRVSERVILAMIGRTGGEEVSAEDDWE
                     MESDPFFKDAGRPRADRGAGAPPGAATGPGETNIFGSSGNSRGRVKSRDGRGGVENDT
                     ETIGSASVRIMRPPAEAGGNGGAATKLERTPTLTNEAVAQLVEAGFSDGTIIRRIEQS
                     PVEFDLSDAKLAELRKRRVGEPVIAAMRAAMSDAAAAPGAKPEE"
     gene            2928..>3377
                     /locus_tag="AC159P_18580"
     CDS             2928..>3377
                     /locus_tag="AC159P_18580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697504.1"
                     /translation="MTRLRLRFVCLLSLCAAAALAAPLVSGARQTGQKQTRPRQVGKP
                     APAQKTPAPANAVADESVGDDEVVKVDSLEVLLPVTVRDASGALVKNLSRADFRVYED
                     GTEQPLSELQARQVPADVALLIDASSSTAESFEDFRQAALDFAALLGE"
BASE COUNT          575 a         1177 c         1205 g          420 t
ORIGIN      
        1 ggaggcttga acccatgcga aaacgtaact tgcttagttt ggttgtcggc gccgccctgt
       61 tggtcggcgg agcgcacttg tcgaacgtgg ccagcgccaa cgacggcggg aaccctgcgg
      121 cgggggcggc ggtcgaagcc ccgcagaaga agaagggcgg cgggttcgcg agcgcgctcg
      181 gggcgccgtt ccgggcgatt ggcaggctgt tcggcggggg caagaaggag aagaaggagc
      241 agccggtcgc cgagaagaag gacgggcaga aagcagagaa ccgggaagac gccaagcccc
      301 gtcgcttgac cgaaaaggac gcggcggact tccaaagcgc gaccatgatg cgggtcaact
      361 accgcgagtc ggcggcggtc gcgcccgcgg cggtggccgc cccccacgcc ctcgtcgccg
      421 aggctcgcgc gctgctcgcg cgcgacgagt tcggcgaggc catctcacgc ctgtcgagcg
      481 ccgcgtccgc cgcccccaac ctcgccgagg cgcaccacct gctcggctac gcctacgacc
      541 gcaagggcct gcaccagctc tcgctcgacg cttaccggcg cgccgtgaag ctcgcgccca
      601 acgacgcgca actgctcaac gactacggct acacgctcta cctgcgcggc cagcacaaag
      661 aggcgaagga gctgctcaag cgggccgcca agcgcgcgcc cggcgacgag cgggtgtgga
      721 acaacctcgc ggtcgtgcag gtcaagctcg gcagctatga cgacgcgttc aagagcttcg
      781 cgcgcgcggg cggcgagttc aaggggcgca tgaacgtcgc caacgcgctg gagcgcgccg
      841 ggcgttacga agacgcgctg aagcactacg agggcgcgcg caaggtcaac cccgcgtcgc
      901 gcgccgcgct gcaacacctg gccgacctct accagcggct gggccggaag gaggaggaag
      961 aggcggcacg ccaactcctc gccgcgccga agcaggagaa gccgaagtcg gaaaacggag
     1021 gccagtagga ggcgacaccg tgcgcgcgct cggtgccggc acgacttcgc gtgaagtgaa
     1081 cgagatgacc cctcgcggct cacaactcaa tcggcgcaac ggaagagcgc gggccttgtt
     1141 cttcaggctc gcgctcgcct gttgtgccct gacgctgctg cccgcggcgg cgctggccga
     1201 ccgcctgcac ctgaagaacg gcgccgtcat cgaggtggac gatgcttggg agaacgccga
     1261 cggcgtctgg tatcgccgcg cgggcgtcgt gcagctcatc gcgaaggatc gcgtgagccg
     1321 catcgagaag agttcgggcg agaaggcggc ggacgtagcg acacccgtcg cgcctgccga
     1381 agtggtgaaa agaggcgccg ccgccgcccc gcgccccgcg caaacacctg cggcgcgaac
     1441 cgtctggatt cacctcgtcg gcggcgcgaa gatcgaggtc gaagaggccg acgagacctc
     1501 cgagggcgtg tggtacaagc gcgcgagttt ttcgatcttc ctcgacaaag agcgcgtcgc
     1561 ccgcatcgag cgcgacgcgc ccacgcccgc cgccgccgct gcgggcgcgc ccgcgagcgc
     1621 gcacagggcc ttcggctgga cgacgggcag cacgcgtctc gacgagctga tccgccggaa
     1681 cggcgcgcgc cacggcgtcg atccctacct catcttctgc gtgatggagc acgagtcgca
     1741 cttcaggtcg cgggccgtct cgcacgcggg ggcgcgcggg ctgatgcagc tcatgcccgg
     1801 caccgcccgc cgcttcggcg tccgcgacgt gttcgacccg gcgcagaacg tcgcgggcgg
     1861 cacgcgctac ctcaaagaac tcctcgacat gtggggcggg cgggtggact tggcgctggc
     1921 gagttataat gccggcgagg gcgcggtgat gaagtacggc tagcgtgtcc ccccctacag
     1981 ggagacgcgc aactacgtgc ggctcatcgg cgcacgctac ggccagagcg ccgccccgac
     2041 cgcgaacgcc cccgcggaca cagccccctg agaatggatt ttccgtgcag gccccgtgcc
     2101 ttgaggtgaa gcgatgccgc gtttactggt atacgccgtt ctgtcagccg ccgtgctcct
     2161 gcaaggcgcc gtcgcgtccc gcgcgcaggc gcaacgcgcg gacgaacagg ttctcactaa
     2221 cgcctccgtc atcaagctcg tgcgcggcgg cttcagcgac aaggccgtca tcgccatcat
     2281 caactcgcgc ccgacccgct tcgacctcgc caccgaacgc ctcatcgagt tgaagaagag
     2341 ccgcgtcagc gagcgcgtca tcctcgccat gatcgggcgc acgggcggcg aagaggtctc
     2401 cgccgaagac gactgggaga tggagtccga cccgttcttc aaggacgcgg gccgaccgcg
     2461 cgcagaccgc ggcgcgggcg cgccgccggg cgcggcgacc ggccccggcg agacgaacat
     2521 cttcggctcg tccggcaact cgcgcgggcg cgtcaagtcg cgcgacggca ggggcggcgt
     2581 cgagaacgac acggagacca tcggcagcgc gtcggtgcgc atcatgcgcc cgcccgccga
     2641 ggccggcggc aacggcggcg ccgccaccaa gctcgaacgc acgccgacgc tcacgaacga
     2701 ggcggtggcg cagctcgtcg aggcgggctt cagcgacggg acgatcatcc gccgcatcga
     2761 gcagtcgccc gtcgagttcg acctgtcgga cgcgaagctg gccgagctgc gcaagcggcg
     2821 cgtcggcgag ccggtcatcg cggcgatgcg tgcggcgatg tccgacgccg ccgcagcgcc
     2881 gggcgcgaag cccgaggagt aaccccttga gaaggcgggc acagttgatg acacggctgc
     2941 gactccggtt cgtctgcctc ctctcgctct gcgccgccgc cgcgctcgcc gcgccgctcg
     3001 tctccggcgc gcggcagacg gggcagaagc agacgcgccc acgccaggtg ggcaaacccg
     3061 cgccggcgca gaagacgccc gcccccgcga acgccgtggc cgacgagagc gtcggcgacg
     3121 acgaggtcgt caaggtggac agcctggagg tgctcctgcc cgtcacggtg agggacgcct
     3181 cgggcgcgct ggtgaagaac ctcagccgcg cggacttcag ggtctacgag gacgggacgg
     3241 agcagccctt gagcgagcta caggcgcggc aggtgccggc ggacgtggcg ctgctgatcg
     3301 acgcctcctc ctcgacggcg gagagcttcg aagacttccg gcaggccgcc ctcgacttcg
     3361 ccgccctgtt gggtgag
//
LOCUS       DBNGOH010000956         5800 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4175312, whole genome shotgun sequence.
ACCESSION   DBNGOH010000956 DBNGOH010000000
VERSION     DBNGOH010000956.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5800)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5800)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5800
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4175312"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            13..867
                     /locus_tag="AC159P_18585"
     CDS             13..867
                     /locus_tag="AC159P_18585"
                     /inference="COORDINATES: protein motif:HMM:NF024834.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR3697505.1"
                     /translation="MWNTRSSRQKAVGSRQLRATAAPRACVFARRAFGFLLLTAFCLL
                     PTGCRMDMQDQPRYEAYEAGDVKYFKDGKASRQLIEGTVPRVAPGQPYVDVQTDYLYT
                     GRPAAAGGAAQGGAQGVQGVRANVPAAGGATQASGQSGPDVFPMAIDEAALRRGQERF
                     NVYCAMCHGMTGEGDGMIVRRGFRKPPSFYEDRLQSPTSSASHLFDVITNGWGAMPSY
                     SDMIPAEDRWKIIAYVRALQLGGKGKLEDVPADKRPALEAGQPTTTHGAMAGEGGEGQ
                     SGQGDKSH"
     gene            941..2176
                     /locus_tag="AC159P_18590"
     CDS             941..2176
                     /locus_tag="AC159P_18590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697506.1"
                     /translation="MDASNYNAPPTVGRYRQRALFVGVAFLLLSAALSFIVGGPVQFF
                     RSYLVAFFFTTGLAVGSLVWLMLGHMTGGGWAIMSRRIFEAATRTLPLMALLFIPLVV
                     SLFVHDHGHSLYEWSDPHHVAEDKVLQYKARYYLNAPFFIVRGVFYFAVWIALAYFMN
                     KWSAQQEKTGDPRIRRKMQDVSGPGILLFGLTVTFAAVDWGMSLEPHWFSTIYGLIAM
                     AGWGLSALAFAITVASFLVKHEPMAEVYAPLHFHDWGKLLLAMVMLYAYFSFSQFLII
                     WSGNLLEEVPFYLRRLRGGWQYVGLAMILFHFALPFALLLSRSLKRHGSTLRRVALLL
                     LFMRVVDLIFLFAPASHQGPGASAGIHATDFLTMFGMTAGLLGVWLFFFFAELAKRPL
                     VPLGEPNLEKALEAAHAHH"
     gene            2265..2891
                     /locus_tag="AC159P_18595"
     CDS             2265..2891
                     /locus_tag="AC159P_18595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697507.1"
                     /translation="MAGGKRKRDSIHETPDVSYITNPDVAHEHTDVPVTPVLKFIAGL
                     VVFAIVMHIAMYAAYLFFEAREKAAERQSSPLARTPQESLPPEPRLQLAPGFEVKTED
                     GKAIDLSYQAVRTGAVPAPQSEYWTVQKEWKRKLENYTWADRQAGAVRLPVDEAIRRY
                     AEQQKAGAAGQPTAPGQAAPQASPSPAAPAEHTPAPSSSGRTTEQKHQ"
     gene            2992..3891
                     /locus_tag="AC159P_18600"
     CDS             2992..3891
                     /locus_tag="AC159P_18600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HIR3697508.1"
                     /translation="MKFGRKSFAGRLLWRLCILLPAACCLLPTVSAQYGRPPESRMPQ
                     GGTPEVLKKVGIEQRLNEQLPLDAVFRDEAGREVRLGEFFKKDRPVILSLVYYECPML
                     CNQILNGMVGSFEGLNFSAGKEFEVVTVSFDPTERPELAARKKETYLKRYGRKGAEAG
                     WHFLTGDKGEIDKVANAVGFGYVWDEGSKQFAHGSAIMVATPAGRLSHYFYGIDYAPR
                     DVKLALVEASAGKVGSPVDQLMLYCYHYDPTTGKFAPVMAVMRGAAAATVAGVVFLVL
                     ALRRRRDGDAGRWGGESKAGGSV"
     gene            3897..5003
                     /gene="coxB"
                     /locus_tag="AC159P_18605"
     CDS             3897..5003
                     /gene="coxB"
                     /locus_tag="AC159P_18605"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HIR3697509.1"
                     /translation="MQLPLPQVRVPFSPEQASSFAGEVDALYLYLVAITLFFSLTITT
                     LIIFFAVRYRRRDPGEVPRPRAGSLPLEVMWTVIPLMIAMTIFAWGASVYFRLYRAPQ
                     EAMEIYVVGKQWMWKFQHATGQREINELHVPLGARVKLTMTSEDVIHSLYVPAFRVKT
                     DVLPGRYTQMWFEATKAGRYYLFCAEYCGTNHSGMGGYVEVMEPTDFQQWLAGGASES
                     AAAQGGKVFQERGCASCHQEDRAGRGPVLKGIFGKPQELSNGQTVTVDEAYVRESILD
                     PHAKLAAGFGPLMPIYRGQLSEEQVLQLIAYIRSLTDDAQPQRGGGAGTVTGGAPSES
                     QRSNPLAPTSPPPQTRRQGAGGGNTNAGAQGPGR"
     gene            5062..>5800
                     /locus_tag="AC159P_18610"
     CDS             5062..>5800
                     /locus_tag="AC159P_18610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HIR3697510.1"
                     /translation="MSTATTPETPRGQDWRGRFRHTNYLNVDYGIKSWLLTRDHKRIA
                     LLYLLSVTFFFFLGGAYAVAIRVELMTPLGDLMQSQTYNKVFTHHGILMVFFFLIPSI
                     PAVLGNFLVPLMIGAKDLAFPRINLLSWYIYMAGGALTFWALLIGGVDTGWTFYTPFS
                     TTFSNTYVVAAGVGIFINGFSSILTGLNFIVTIHTMRAPGMTWFRLPLFIWSHYATSL
                     IMILGTPVVAITVLLVALERLARVGIFD"
BASE COUNT          928 a         1932 c         1969 g          971 t
ORIGIN      
        1 gcgaggtgaa cgatgtggaa cactaggagc agtaggcaga aggcagtagg cagtaggcag
       61 ttgcgcgcca ccgccgcgcc gcgcgcctgt gtcttcgcgc gccgcgcttt cggattttta
      121 ctgcttactg ccttctgcct actgcctacc ggttgccgga tggacatgca ggatcagccg
      181 cggtacgagg cctacgaggc gggggacgtg aagtatttca aggacgggaa ggcttcgcgg
      241 cagttgatcg agggcaccgt gccgcgcgtc gcgccggggc agccgtacgt tgacgtgcag
      301 acggactacc tctacacggg ccgccccgcg gcggcgggcg gtgccgcgca gggcggcgcg
      361 cagggcgtgc agggcgtgcg cgcgaacgtg ccggcggcgg gcggcgcgac gcaggcgagc
      421 gggcagagcg ggccggacgt gttcccgatg gcgattgacg aggcagcctt gcggcgcggg
      481 caggagcgct tcaacgtcta ctgcgcgatg tgccacggca tgacgggcga gggcgacggg
      541 atgatcgtgc gccgagggtt ccgcaagccg ccgtcgttct acgaagaccg cttgcagtcg
      601 ccgacctctt cggccagcca cctcttcgat gtcatcacca acggctgggg cgcgatgcct
      661 tcctactcgg acatgatccc ggcggaagat cgctggaaga tcatcgccta cgtgcgcgcg
      721 ctgcaactgg gcggcaaggg caagctcgaa gacgtgccgg cggacaagcg cccggcgctc
      781 gaagcggggc agccgacgac gacgcacggc gcgatggcgg gcgagggcgg cgagggccag
      841 tccgggcagg gcgacaagag ccactgagcg gcgggcgcaa gcggcgaggg catcgacgag
      901 gcgggccgaa ggcgagccgc aaggacgaga atcactgaag atggacgcga gcaactacaa
      961 cgcacctcca accgtcgggc gctaccggca gcgcgcgctc ttcgtcggcg tggccttcct
     1021 gctgctgtcc gccgccctct cgttcatcgt cggcggcccc gtccagttct tccgctcgta
     1081 tctggtcgcc ttcttcttca cgacgggcct cgcggtcggg agcctcgtct ggctgatgct
     1141 cggccacatg acgggcggcg ggtgggccat catgagccgc cgcatcttcg aggccgcgac
     1201 gcgcacgctg ccgctcatgg cgcttctctt catcccgctc gtcgtctcgc tcttcgtcca
     1261 cgaccacggg cactcgctct acgagtggtc cgacccgcac cacgtcgccg aagacaaggt
     1321 cttgcagtac aaggccaggt attacctcaa cgccccgttc ttcatcgtgc gcggcgtctt
     1381 ctacttcgcc gtgtggatcg cgctcgccta cttcatgaac aagtggtcgg cgcagcagga
     1441 gaagacgggc gacccgcgca tccggcgcaa gatgcaggac gtgtcggggc cgggcatcct
     1501 gctcttcggc ctgacggtga cgttcgccgc cgtggactgg ggcatgtctc tggagccgca
     1561 ctggttctcg accatctacg ggctgatcgc gatggccggg tggggcctgt cggcgctggc
     1621 cttcgccatc acggtcgcgt cgttcctcgt caagcacgag ccgatggccg aagtgtacgc
     1681 gccgctccac ttccacgact ggggcaagct cctgctggcg atggtgatgc tctacgccta
     1741 cttctccttc tcgcagttcc tcatcatctg gtcggggaac ctgctggaag aggtgccgtt
     1801 ctacctccgt cgtttgcgcg gcgggtggca gtacgtcggc ctcgccatga tcctcttcca
     1861 cttcgcgctc cccttcgcgc tgctgctctc gcgcagcctg aagcggcacg ggagcaccct
     1921 gcggcgcgtc gcgctgctgc tcctgttcat gcgcgtcgtg gacttgatct tcctcttcgc
     1981 gccggcctcg caccaggggc cgggcgcgag tgccggcatc cacgcgaccg acttcctgac
     2041 gatgttcggc atgacggcgg ggctgctcgg cgtctggctc ttcttcttct tcgccgagct
     2101 ggccaagcgc ccgctcgtcc cgctcggcga gccgaacctg gagaaggctc tggaggcggc
     2161 gcacgcacat cactgaagaa agtgagcggt gagcagcagg cggaagatga agagacgagc
     2221 agcgaagtga agaagagttg tttctgagtc tggagctttg agatatggcc ggaggcaaac
     2281 gcaagcggga cagcatccac gagacgccgg acgtgtcgta catcacgaac ccggacgtgg
     2341 cgcacgagca cacggacgtg cccgtcacgc ccgtcctcaa gttcatcgcg gggctggtgg
     2401 tcttcgccat cgtgatgcac atcgcgatgt acgcggcgta cctcttcttc gaggcgcgcg
     2461 agaaggccgc cgagcgccag tcctcgccgc tcgcgcgcac gccgcaggag agcctgccgc
     2521 ccgagccgcg cctgcaactc gcccccggct tcgaagtcaa gaccgaggac ggcaaggcca
     2581 tcgacctctc ctatcaggcc gtgcggacgg gcgcggtacc cgcgccgcag tccgaatact
     2641 ggacggtgca gaaagagtgg aagcggaagc tggagaacta cacctgggcc gaccggcagg
     2701 cgggcgccgt ccgcctcccc gtcgatgagg ccatccgccg ctacgccgag cagcagaagg
     2761 ccggcgccgc gggacagccg accgcgcccg gtcaggccgc gccgcaggcc agcccttcgc
     2821 ccgccgcgcc cgccgagcac acgcccgcgc cttcgagttc gggccgcacg acggaacaga
     2881 aacatcagta ggcagcgggc agtaggcagg aggcaggaag aaaaagcagt cggcggtttt
     2941 tgaatcgtca gttcctgagt tcggcagttc ataagatcga gaagtggctt tatgaagttc
     3001 ggaagaaaga gctttgcggg gcggttgttg tggcggctgt gcattctgct gcctgctgcc
     3061 tgctgcctac tgcctactgt ttccgcgcaa tacggcaggc cgccggagag ccggatgccg
     3121 cagggcggga cgccggaggt gctgaagaag gtcggcatcg agcagcgcct gaacgagcag
     3181 ttgccgctcg acgccgtgtt ccgcgacgag gccgggcgcg aggtgcgcct cggcgagttc
     3241 ttcaagaaag accggcccgt catcctgtcg ctcgtgtatt acgagtgccc gatgctgtgc
     3301 aaccagattc tgaacgggat ggtcggctcg ttcgaggggt tgaatttttc ggcgggcaag
     3361 gagttcgagg tcgtgaccgt gagcttcgac ccgacggagc ggcccgagct ggccgcgcgc
     3421 aagaaggaga cctacctcaa gcgctacggg cgcaaggggg ccgaggcggg ctggcacttc
     3481 ctgacgggcg acaagggcga gatcgacaag gtggcgaacg cggtcggctt cggctacgtg
     3541 tgggacgagg ggtcgaagca gttcgcgcac ggcagcgcga tcatggtcgc cacgcccgcc
     3601 ggtcgcctct cgcactactt ctacggcatc gactacgcgc cgcgcgatgt gaagctggcg
     3661 ctcgtcgagg cgtccgccgg caaggtcggc tcgcccgtcg atcaactgat gctctactgc
     3721 taccactacg acccgacgac cggcaagttc gcgcccgtca tggcggtgat gcgcggggcg
     3781 gcggcggcca cggtcgcggg ggtggtgttc ctcgtgctcg ccctgcggcg ccggcgcgac
     3841 ggcgacgccg ggcggtgggg cggcgagtcc aaagcaggag gcagcgtcta agggtcatgc
     3901 agctaccgct accgcaagtg agggtgccgt tctcgcccga gcaggcttcg tcgttcgccg
     3961 gcgaggtgga cgcgctctac ctctacctcg tggccatcac gctcttcttc tcgctgacga
     4021 tcacgacgct catcatcttc ttcgccgtcc gctaccggcg gcgcgacccc ggcgaggtgc
     4081 cgcgcccgcg cgccggctcg ctcccgctcg aagtgatgtg gacggtcatc cctctgatga
     4141 tcgccatgac catcttcgcg tggggcgcga gcgtctactt ccgcctctac cgcgcgccgc
     4201 aggaggcgat ggagatttac gtcgtcggca agcagtggat gtggaagttc cagcacgcga
     4261 ccggccagcg cgagatcaac gagctgcacg tccccttggg cgcgcgcgtc aagctgacga
     4321 tgacgagcga ggacgtgatc cacagcctct acgtcccggc cttccgcgtg aagacggacg
     4381 tgctgcccgg tcgctacacg cagatgtggt tcgaggcgac gaaggccggg cgctactacc
     4441 tcttctgcgc agagtactgc ggcaccaacc actcggggat gggcggctac gtcgaggtga
     4501 tggagccgac cgacttccag cagtggctcg ccgggggcgc gtccgagtcg gcggcggcgc
     4561 agggcggcaa ggtcttccag gagcgcggct gcgcctcgtg ccaccaggag gacagggcgg
     4621 gccgcggccc cgtgctcaag ggcatcttcg gcaagccgca ggagctttcg aacggccaga
     4681 ccgtgacggt ggacgaggcg tacgtccgcg agtcgatcct cgacccgcac gccaagctcg
     4741 ccgcgggctt cgggccgctc atgccgatct accgcgggca gctcagcgag gagcaggtct
     4801 tgcagctcat cgcctacatc cgctcgctca ccgacgacgc gcagccgcag cgcggcggcg
     4861 gcgcgggcac cgtgacgggg ggcgccccgt ccgagtcgca gcgctcgaac ccgctcgccc
     4921 cgacctcgcc gccgccgcaa acgcggcggc agggcgcggg cggcggcaac acgaacgcgg
     4981 gcgcgcaggg gccgggccgt tgatgacggg agcggaaggg ttgtcttctt cgaccgccgg
     5041 gtacttcttg gtaaagcatt tatgagcaca gcaaccacac cggagacgcc gcggggtcag
     5101 gactggcggg gcaggttccg gcacacgaac tacctcaacg tcgattacgg catcaagtcg
     5161 tggctcctga cgagagatca caagcgcatc gcgctgctct acctgctctc ggtcacgttc
     5221 ttcttcttcc tcggcggggc ctacgccgtg gccatccgcg tcgagctgat gacccccctg
     5281 ggcgacctga tgcagtcgca gacctacaac aaggtcttca cgcaccacgg gattctgatg
     5341 gtcttcttct tcctcatccc gtcgatcccc gccgtgctcg gcaacttcct cgtgccgctc
     5401 atgatcggcg ccaaagacct cgccttcccg cgcatcaacc tgctgagctg gtacatctac
     5461 atggcgggcg gcgcgctcac cttctgggcc ttgctcatcg gcggcgtgga caccggctgg
     5521 acgttctaca cgcccttctc gacgaccttc tcgaacacct acgtggtggc cgcgggcgtc
     5581 ggcatcttca tcaacgggtt ctcgtccatc ctgacggggc tgaacttcat cgtcaccatc
     5641 cacaccatgc gcgcgccggg catgacgtgg ttccgcctgc cgctcttcat ctggtcgcac
     5701 tacgcgacga gcctgatcat gatcctcggc acgccggtcg tcgccatcac ggtgctgctg
     5761 gtcgcgctcg aacggctcgc ccgcgtcggc atcttcgacc
//
LOCUS       DBNGOH010000957         2608 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_5093856, whole genome shotgun sequence.
ACCESSION   DBNGOH010000957 DBNGOH010000000
VERSION     DBNGOH010000957.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2608)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2608)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2608
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_5093856"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(191..559)
                     /locus_tag="AC159P_18615"
     CDS             complement(191..559)
                     /locus_tag="AC159P_18615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697511.1"
                     /translation="MTARRRGMRRGAKLMFICAVLLPAVTLLAFEGDAPGPLLLVCTA
                     FLAGLAWFVYSLLFNDDTVPVGKGATHRDLGAGGQRPALGAPQFTPAPLFNQQRANTA
                     ELYQPPSITENTTKLLDKDS"
     gene            complement(885..1475)
                     /gene="dcd"
                     /locus_tag="AC159P_18620"
     CDS             complement(885..1475)
                     /gene="dcd"
                     /locus_tag="AC159P_18620"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008063044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006229 - dUTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HIR3697512.1"
                     /translation="MTVKSDRWIRQMAEEHKLIDPFNATLVREVEGRRIISAGASSYG
                     YDMRLADDGFRVFSPIHGREIDPKRFDEESLVEPPLRAAEDGSRYYLMPPHSYALGVT
                     VETFRMPRNVTGICMGKSTYARAGLLVNTTPLEAGWQGRLVIELGNLADLPIRVYVGE
                     GIGQVLFFQSDDDCDVSYQDRGGKYQGQTGLTYSRL"
     gene            complement(1765..>2608)
                     /locus_tag="AC159P_18625"
     CDS             complement(1765..>2608)
                     /locus_tag="AC159P_18625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HIR3697513.1"
                     /translation="VSARRIVAQEASKKSAAAPQAPLLKRTATRRETKRLGHGGTLTL
                     SGAPEGSVSVESWPKNEAEVVAEIEVSAHTEDELARLAAVSGFLLESDFNRLGVTTVG
                     THDRKYMKRVARDFPKKLLTMPWRADYRLRVPSIVDLEIATGRGPVTLSHLDGSVRLD
                     AGGGFPSLFTLAGGDFEGTVAAGPVTLRVPARSWRGRGLTLRLVSGDLTLELPANFDG
                     DIDADVLRAGRIENTHPNLSPREDAPAPTERSLRARAGNGGAPLSLTVGDGTLRIRQA
                     STQQ"
BASE COUNT          412 a          776 c          982 g          438 t
ORIGIN      
        1 cgctcggcca tccagttgcc ccacgctttg cagtcgttga agaagggcgg gtgcgaggcg
       61 aactgctcct ccgtcatgcc cggccagtat ccattgacgg ttgcgcccat caagtctcct
      121 cctgcgatac gaacccgaac gtgccgcccc cccgcccgct cgacccgtcc gtgaacagat
      181 gcgagggggt tcaggagtcc ttgtcgagca gcttcgtcgt gttctcggtg atgctgggcg
      241 gctggtacag ctcggcggtg ttcgcgcgct gctggttgaa gagcggcgcg ggggtgaact
      301 gcggcgcgcc gagggccggc ctctgcccgc cggcgccgag gtctctgtgc gtcgcgcctt
      361 taccgacggg cacggtgtcg tcgttgaaca gcagggagta gacgaaccac gccagcccgg
      421 ccaggaacgc ggtgcagacg agcagcagcg gtccgggcgc gtcgccctcg aaggcgagca
      481 gcgtgacggc gggcagcagg acggcgcaga tgaacatcag cttcgcgccg cgccgcatgc
      541 cgcgccgccg cgcggtcatc accggcgcgc gcgggacagg ggccccgtcc ggggcatgct
      601 cgttggccgc tatgtaggcc gcgagcccat cgagctggag cctgcacctc gggcagaagc
      661 gaacttcgtc tgaagactgc tgaccgcact tgggacaaaa catccggttc acctgtggct
      721 cgggcggcgg tttggactac tgccggcatt acgcagccgc gcgccgctaa gttctgaagt
      781 cgggcgcccg cctcggcgcc gtcccgtata atagcagccg cgacgccgac actgaactta
      841 gcggcaagtc cgattcagga tttgtcattc aacctacgac gacatcataa gcgcgagtag
      901 gtgaggccgg tttggccctg gtatttgccg ccgcggtcct ggtaggagac gtcgcagtcg
      961 tcgtcggatt ggaagaagag gacctggccg atgccttcgc cgacgtagac gcggatgggg
     1021 aggtcggcga ggttgccgag ttcgatgacg aggcggccct gccagccggc ttcgaggggc
     1081 gtggtgttga caagcaggcc cgcccgcgcg taggtggatt tgcccatgca gatgccggtg
     1141 acgttgcggg gcatgcggaa ggtctcgacg gtgacgccga gggcgtagga gtggggcggc
     1201 atgaggtagt agcgcgagcc gtcttcggcg gcgcgcaaag ggggttcgac gagcgactcc
     1261 tcgtcgaagc gtttggggtc gatctcgcgc ccgtggatgg gggagaagac gcggaagccg
     1321 tcgtcggcga ggcgcatgtc gtagccgtag gaggaggcgc cggcggagat gatccggcgg
     1381 ccttcgactt cgcggacgag cgtcgcgttg aaggggtcta tgagcttgtg ctcctcagcc
     1441 atctggcgga tccagcggtc ggatttgaca gtcatcgttt taagtgtccg gggacgtgga
     1501 agagatttcg atcagagcgc gcgccgcggc gggcggcagc gcgaagcata gcagatttaa
     1561 gaacggtagg cagtaggcag caggcggtag gcagttaaag caagaagtca gaagtcagaa
     1621 gtcagaagtc agaagtcgga agagggagag gggcttggga ggtaaagacc tgagcctctc
     1681 cgctcttcaa gcaacagagc aggaggcttg agacgttagc tctcaagcct cctgctttaa
     1741 ctgcctactg cctactgcct actgttattg ctgagtgctc gcttgcctga tgcggagcgt
     1801 gccgtcgccg acggtgaggg agagcggggc gccgccgttg ccggcgcggg cgcgcaggct
     1861 gcgctctgtg ggcgcgggcg cgtcttcgcg gggcgagagg ttggggtgcg tgttctcgat
     1921 gcggcccgcg cgaagcacgt cggcgtcgat gtcgccgtcg aagttggcgg gcagctccag
     1981 cgtgaggtcg ccgctgacga ggcggagcgt gaggccgcgc ccgcgccagc tccgcgcggg
     2041 aacgcgcagc gtgacggggc cggcggcgac ggtgccctcg aagtcgccgc ccgcgagcgt
     2101 gaagagcgag gggaagcctc cgcccgcgtc gagccgcacg gagccgtcga ggtgcgagag
     2161 ggtgacgggg ccgcggcccg tggcgatttc gagatcgacg attgacggca cgcgcaggcg
     2221 gtagtcggcg cgccagggca tcgtgagcag ttttttgggg aagtcgcggg cgacgcgctt
     2281 catgtacttg cggtcgtgcg tgccgacggt ggtgacgccg aggcggttga agtcggattc
     2341 gaggaggaag ccgctgacgg cggcgagccg cgccagctcg tcttcggtgt gggcgctgac
     2401 ctcgatctcg gcgacgacct cggcctcgtt cttcggccaa ctctcgaccg aaacggagcc
     2461 ttcgggcgcg ccgctcagcg tgagcgtgcc gccgtggccg agccgcttcg tctcgcgccg
     2521 cgtcgcggtg cgcttcagga gcggcgcctg cggggcggcg gcgctcttct tcgacgcttc
     2581 ctgtgcaact atccggcggg cgctgacc
//
LOCUS       DBNGOH010000958         9632 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1428003, whole genome shotgun sequence.
ACCESSION   DBNGOH010000958 DBNGOH010000000
VERSION     DBNGOH010000958.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 9632)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9632)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9632
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1428003"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            102..2636
                     /locus_tag="AC159P_18630"
     CDS             102..2636
                     /locus_tag="AC159P_18630"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013239.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HIR3697514.1"
                     /translation="MNTQLCRRSRTLLALAFVFFALPVETSAAWRAAGDVRGVERVPD
                     GVVLTLTSGARVAVTFRDIETVRVRLAPTGKFERDFSYAVESKDRKTVAVKIEETPAQ
                     IKVSSLDGTAVVIRRRPFLLTVLDPAGRAVVEDDPARPHSIDPETGAAETSKRRPEWE
                     TYYGFGEKAMPMSRHMQHLVMWNTDTYGYPRGLDPIYQSIGFFVALRREQDKGNAYGL
                     FLDNTTRTYFDMGKTDPARYTFGVASGELNYYVFTGGRERSPKNILRDYTNLTGRTPL
                     PPMWALGNQQSRWSYTPESKVREVAKQFRETRTPLDVLYLDIDYMDGYRVFTWDKTRF
                     PDPSKLVGDLRKDGIRTVLIIDPGVKYDENYHVYKEGKAGGHFVKAADGTEFRASVWP
                     GVCAFPDFTDPKAREWFGSYYKQHLDEGIAGFWNDMNEPATFLNETTPKPDIYHHPLK
                     TFPVTVRHAGDGQPGTHARYHNVYGMQMARSTFEALKRLRPDERPFVLTRAGYAGVQR
                     FSAIWTGDNIASWDHLRLSIPMLTNLGVSGVALVGADVGGFSENPSPELYARWLQAAA
                     LTPFLRSHSEFVSKPHEPFAYGPEFTDINRATIELRYKLLPYIYSLFHEHTETGAPPM
                     RPLWFEYPDDVRTYLVEDQYLVGRDLLVAPVVTEGVVKRRVYFPKGSAWVDWWTGRRH
                     EGGTDAEVDAPLDRLPLFARVGATIPTQAPVQHTGEMPSVALKLVTVRGADGTSSFYE
                     DAGEGYDYRRGDHRTTIAIQFANKLRLFRSGNYKSPMPRSVASVEFLGVKSPPREVWA
                     AGRAVGDISFDAATRRLLIPLAAGVNEVVIDPTFIVSTRDEGNTRP"
     gene            2633..2809
                     /locus_tag="AC159P_18635"
     CDS             2633..2809
                     /locus_tag="AC159P_18635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697515.1"
                     /translation="MNPSTDRHDLDRRRFISSIGKGLGLAALSSTTVASLLNEVRAAA
                     RGSPSARRCSSTWG"
     gene            2800..3132
                     /locus_tag="AC159P_18640"
     CDS             2800..3132
                     /locus_tag="AC159P_18640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697516.1"
                     /translation="MGLGPARKEARLRYLSRRWMNRLKALPRVGFNTSFDDAQSCALA
                     NFHLEGTDPVALGSYLMNTHRIFTTPIVHDEFKGVRITPNLYTTLAELDRFADVVETV
                     ARKGLHKT"
     gene            3444..8939
                     /locus_tag="AC159P_18645"
     CDS             3444..8939
                     /locus_tag="AC159P_18645"
                     /inference="COORDINATES: protein motif:HMM:NF013276.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HIR3697517.1"
                     /translation="MRTLKTLRVARRLKTHLLVVVAAALALPFVAAAAVLVNDTFADG
                     SSANQNLAAHSIRIFKGRTATVRNDAVGSVTFDMTATGTSAEAFWGFFTNSGSPVNLG
                     VGDKLSVSGTFSLTGFVAGGQDIRFGILNSLGTRNANDLTGGMNDASFANDPGYGLQF
                     IPSGTGAPFVINRRTVVTGNNPFNSIGDFTAIPGTGATTRQTLANETPYTLTYTIERL
                     TATDTRISVSVTGGTLPANFSFTSVESSTTPNTSFDYFGFRVGGNAFAQKIKFTNWLI
                     DYTPSLPVITAQPQPSNLTVQVGSNVTTSVAASGNSLAYQWHKDGAPVVGNPSASTPT
                     LNLTNVQLSDAGSYTAVVSNPGGSTTSNPVTLNVSVDPVPPPPSITAQPLDTTAPVGS
                     PASLTVAATGDNLFYQWFKNGTLINGATSAALDFASAQVADSADYRVVVSNSSGSVTS
                     LAARLLVVSTMQAASFAPSNGTSDVNIDAPLYLTFNQPPAPGTTGRLRVFREDGTVVE
                     TIDMSAATQSRLNGTVSFNYRPIITSGNTAAIYLHQKLAYNQSYYVTMEPGVIRDAAG
                     APFVGFAENTVWNFSTKTSGPAAGSVALTVAADGTGDFSTVQGAIDFVPANNTRRVVI
                     TVKKGTYTEIVYVNSNKPFITVRGEDRALSIVSYANNNNFNPTSTTTRAMFGVDAPDF
                     TLENITLHNTTPQGGSQAEAFRGNNNRIVLNRVNLKSFQDTLLLQGQSNQGGFVTDSY
                     IEGDVDFMWGGGPVFFQNTELKMLRTDAFYIQSRNVSGRNGFVYVNCKFTRAAGINGS
                     FLNRIDPDDFPFSQVVIINSQMDGVRPDPWQFNNPTNAVNAANYPNIRMWEYNSRDLS
                     GNLVDVSQRHPISRQLTAAEAAQWSDPAFVLGGWAPQTKLTAAVSLSNLTQNFNGSPA
                     VVTATTEPAGLPVTVTYNGSTTPPSAIGSYAVVAIVDDATYQGTANGTLVIDRTPVSV
                     TLSNLVHTYDGTPKSASVGTNVPGPVVSVTYNGSTAPPTEVGTYSVVAAINDPNYRGG
                     TSATMTIYAPGAQPLKAFPGAEGAGELSRGGRGGDVYHVTNLDDSGAGSLREGIRTAS
                     GPRTIVFDVSGTIHLNSRLTINKPFLTIAGQTAPGDGVTIAGWPTVITNTNNVVVRYL
                     RFRVGDANCPVVQDDALWVDKATDIILDHVSASWSVDETLSVTESDRVTVQWSFITES
                     MKNSCHEKGAHGYGSLIRFGNGVVTYHHNLYAHHDSRNPRVGDQIGLDFVNNVIYNWG
                     GESGYSGPAEEGTTRVNYVGNYLVAGPQTTASKLRAFSGGSANTQIYQSNNFLDGDKD
                     GARDGADNGWTMFVGSYTKREPARFDFPQVTTHDAATAYERVLGTAGHSRSRDSVDTR
                     VVGEVRGEGGHHINSQNDVGGWPVLDSTAAPADTDGDGIPDEWEINHGLNPADAADGR
                     ATTASGYTNLERYLNDIVPVPGVDPSTDSAAPTATATLSHEGNAAGWHNSDVTVTLAA
                     ADNDGGSGLREITYSVNGAFAYANTSPITIPVNIEGTTTITFYAGDLAGNTGAAQVLT
                     VKLDKTAPVVDTTRAPEANANGWNNTDVVAGYSATDAVSGFGDGPDESGAYTFSSEGA
                     GQSFTFQVTDLAGNAASATVADVSIDKTAPVVHLRRPVEGAFYLLGQAALADYDCADN
                     LSGLASCAGTVAANSLFDTATVGGKTFAVNAADLAGNTAAQAVGYTVGYQLNLLYDAN
                     KVHRGGSTVPVKLQLFDSAGANLSSAATVLHAVGVSLVTPETSGTAATDAGNSNPDSA
                     FRFDPSLGGTGGYQFNLKTDGLAPGTYRLSFTVGGDPHVYAAQFQIR"
     gene            complement(9058..>9632)
                     /locus_tag="AC159P_18650"
     CDS             complement(9058..>9632)
                     /locus_tag="AC159P_18650"
                     /inference="COORDINATES: protein motif:HMM:NF037660.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3697518.1"
                     /translation="AEALDEKEGMANAYLGLGIVSERQRKFAEAEEAYTRALKLSRSL
                     GSKAGMERAHGNLGNLHVSQGELRKAKRMYEAALSMAKALGHKDGMALTYVNLADIEE
                     AGGERDEARELLTRALELYQQIGNKGEVKKLSARLNPKPAKKKSKRRAMRSMRPSYSV
                     QGGLRKLARKGASKKSGVRPYRPFVGAKKR"
BASE COUNT         1717 a         3724 c         2720 g         1471 t
ORIGIN      
        1 gtccctttct tgatcgtttc tttctttcgt gtcttcgtgt cttcgtggtt gaatcatccg
       61 ttcttcgacc acgcaacttc atccgaacca cgggccacgt catgaacacc caactttgcc
      121 gccgcagccg cacgcttctc gcgctcgcct tcgtcttctt cgccctcccc gtcgagacct
      181 ccgccgcgtg gcgcgcggcg ggcgacgtgc gcggcgtcga gcgcgtgccg gacggggtcg
      241 tgctcacgct cacttcgggc gcgcgcgtcg cggtcacgtt ccgcgacatc gagacggtgc
      301 gcgtgcgcct cgcgccgacg ggtaagttcg agcgcgactt ctcctacgcc gtcgagtcga
      361 aagaccgcaa gaccgtcgcc gtcaagatcg aagagacgcc cgcgcagatc aaagtctctt
      421 cgctcgacgg cacggcggtc gtcatccgcc gccgcccgtt cctgctgacc gtgctcgacc
      481 cggcgggccg ggcggtcgtc gaagacgacc ccgcgcgccc ccactccatc gaccccgaga
      541 cgggcgccgc cgaaacctcg aagcgccgcc cggagtggga gacctactac ggcttcggcg
      601 agaaggcgat gccgatgtcg cgccacatgc agcacctcgt gatgtggaac accgacacct
      661 acggctaccc gcgcggcctc gaccccatct accagtccat cggcttcttc gtcgcgctcc
      721 gccgcgaaca ggacaaagga aacgcctacg gcctcttcct cgacaacacg acgcgcacct
      781 acttcgacat gggcaagacc gaccccgcgc gctacacctt cggcgtcgcg tcgggcgagc
      841 tgaactacta cgtcttcaca gggggccgcg agcgctcgcc caaaaacatc ctgcgcgact
      901 acacgaacct cacgggccgc acgcccctgc cgcccatgtg ggcgctcggc aaccagcagt
      961 cgcgctggtc gtacacgccc gagtcgaagg tgcgcgaggt ggccaaacaa ttccgcgaga
     1021 cgcgcacgcc gctcgacgtg ctctacctcg acatcgacta catggacggc taccgcgtct
     1081 tcacgtggga caagacgcgc ttcccagacc cctcgaagct cgtcggcgac ctgcgtaaag
     1141 acggcatccg caccgtcctc atcatcgacc ccggcgtcaa gtacgacgag aactaccacg
     1201 tctacaagga gggcaaggcg ggcggccact tcgtcaaggc ggcggacggc accgagtttc
     1261 gcgccagcgt ctggcccggc gtctgcgcct tccccgactt caccgacccg aaggcgcgcg
     1321 agtggttcgg ctcctactac aagcagcacc tcgacgaagg catcgccggt ttctggaacg
     1381 acatgaacga gcctgcgacc ttcctcaacg agacgacgcc gaagcccgac atctatcacc
     1441 acccgctgaa gaccttcccc gtcacggtcc ggcacgcggg cgacggccag cccggcacgc
     1501 acgcgcgcta ccacaacgtc tacggcatgc agatggcgcg ctccaccttc gaggctttga
     1561 agagactcag gccggacgag cgacccttcg tgctgacgcg cgcgggctac gcgggcgtgc
     1621 agcgcttctc ggccatctgg acgggcgaca acatcgcctc ttgggatcac ctgcggctct
     1681 cgatcccgat gctcacgaac ctcggcgtct cgggcgtcgc gctggtcggc gcggacgtgg
     1741 gcggcttctc cgaaaacccc tcgcccgaac tctacgcgcg ctggcttcag gccgcggcgc
     1801 tcacgccctt cctgcgctcg cactccgagt tcgtctccaa gccccacgag cctttcgcct
     1861 acggccccga gttcaccgac atcaaccgcg cgaccatcga gctgcgctac aaactgctgc
     1921 cctacatcta ttccctcttc cacgagcaca cggagaccgg cgcgccgccg atgcgcccgc
     1981 tctggttcga gtaccccgac gacgtgcgca cctacctcgt cgaagaccag tacctcgtcg
     2041 ggcgcgacct gctcgtcgcg ccggtcgtca ccgagggcgt ggtgaaacgc cgcgtctatt
     2101 ttccgaaggg cagcgcgtgg gtcgattggt ggacgggccg gcgccacgag ggcggcaccg
     2161 acgccgaggt tgacgcgccg cttgaccggc tgcccctctt cgcacgcgtc ggcgccacca
     2221 tccccacgca agcgcccgtg cagcacacgg gcgagatgcc gagcgtggcg ctgaagttgg
     2281 tgacagttcg cggcgccgac ggcacaagca gtttctacga ggacgcgggc gaaggctacg
     2341 actaccgacg cggcgaccac cgcacgacca tcgccatcca gttcgccaac aagctccgcc
     2401 tgttccgctc gggaaattac aagtcgccga tgccgcgctc cgtcgcgtcg gtcgaattcc
     2461 tcggcgtcaa aagcccgccg cgcgaagtgt gggccgccgg tcgcgccgtc ggcgacatct
     2521 ctttcgacgc cgcgacgcgc cgcctgctga ttccgctcgc ggcgggggtg aacgaagtgg
     2581 tcatcgaccc cacgttcatt gtcagcaccc gcgacgaagg gaacacgcga ccatgaaccc
     2641 aagcaccgac cgccacgacc tcgaccgccg ccgcttcatc tcttccatcg gcaagggact
     2701 cgggttagcg gccctctcat caacgacggt cgcctcgctc ttgaacgaag tgcgcgccgc
     2761 cgcccgcggc tcgccatcgg cgaggcgctg ctcttccaca tggggctagg ccccgcgcgc
     2821 aaagaggcgc gcctgcgcta cctctcgcgc cgctggatga accgcctcaa ggcgctgccc
     2881 cgcgtcggct tcaacacctc cttcgacgac gcgcagtcct gcgccctcgc caacttccac
     2941 ctcgaaggca cagaccccgt cgccctcggc tcctacctca tgaacaccca caggattttc
     3001 accacgccca tcgtccacga cgaattcaaa ggcgtccgca tcacccccaa cctctacacc
     3061 acgctcgccg aactcgaccg cttcgccgac gttgtcgaaa ccgtcgcccg aaaaggcttg
     3121 cacaagacat gaaaagaagt actgagtact gagtactgag tgaaagacag attgctttta
     3181 ctcagtactc tgtactcagt acttcttttc tcctgccgaa tccgccaaga aaatagtccg
     3241 acccaaccaa gaaaacgccg cgcgtcccgg ctataatccg ccgtccctta acttcccccg
     3301 gtgtggtttg gcacgcagcg caagacctca ctaaagccgg caacgtcgat atcaagctca
     3361 tgttcgcggg cgcgccgctg ccgcgcgccg ccgcccttaa ccgcacttta gtcgccggac
     3421 ttgaaggagc cgagaacccg aaaatgagaa cactgaagac gctgagagtc gcccgcagac
     3481 tgaagacgca cctgctcgtc gtcgtggccg ctgcgctggc gctgccgttc gtggccgccg
     3541 ccgccgtgct cgtcaacgac acgttcgccg acgggagcag cgccaaccag aacctcgccg
     3601 cccactcgat caggattttc aaaggccgca ccgccaccgt gcgcaacgac gccgtcggct
     3661 ccgtcacctt cgacatgacc gcgaccggca cctcggcgga agccttctgg ggcttcttca
     3721 ccaactccgg ctcgcccgtc aacctcggcg tcggcgacaa gctctcggtc tccggcacct
     3781 tctcgctcac aggcttcgtc gcgggcgggc aggacatccg cttcggcatc ctgaactcgc
     3841 tcggcacgcg caacgcgaac gacctcaccg gcggcatgaa cgacgcgtca ttcgccaacg
     3901 accccggcta cggcctgcaa ttcatcccga gcggcaccgg cgcgcccttc gtcatcaacc
     3961 gccgcaccgt cgtcaccggc aacaacccct tcaacagcat cggcgacttc acggcgatcc
     4021 ccggcacggg ggcgaccacc cggcagaccc tcgccaatga gacgccctac acgctgacct
     4081 acaccatcga gcgattgacg gccaccgaca cgcgcatctc cgtctcggtc acgggcggga
     4141 cgctccccgc caacttttcg ttcacctcgg tcgagtcgag cacgacgccc aacacctcgt
     4201 tcgactactt cggcttccgc gtcggcggca acgccttcgc gcagaagatc aagttcacca
     4261 actggctcat cgactacacg ccctcgctcc ccgtcatcac cgcgcagccg cagccctcga
     4321 acctgaccgt gcaggtcggc agcaacgtca ccacctcggt cgccgcgagc ggcaactcgc
     4381 tcgcctacca gtggcacaag gacggcgcgc ccgtcgtcgg caacccctcg gcctcgacgc
     4441 ccacgctcaa cctcacgaac gtccagctct ccgacgcggg cagctacacc gcggtcgtct
     4501 cgaaccccgg cggcagcacg acgagcaatc ccgtcacgct caacgtcagc gtcgatccgg
     4561 ttccgccgcc gccctcgatc accgcgcagc ccctcgacac gaccgcgccc gtcggcagcc
     4621 ccgcctcgct cacggtcgcg gcgacaggcg acaacctctt ctaccagtgg ttcaagaacg
     4681 gcacgctcat caacggcgcg acctcggcgg cgctcgactt cgcctcggcg caggtcgcgg
     4741 actccgccga ctaccgcgtc gtcgtcagca actccagcgg cagcgtgacg agcctcgcgg
     4801 cccgcctgct cgtcgtctcg acgatgcagg ccgcctcgtt cgcgccctcg aacggcacct
     4861 cggacgtgaa catcgacgcg ccgctctacc tgaccttcaa ccagccgccg gcccccggca
     4921 cgacgggccg cctgcgcgtc ttccgcgagg acggcaccgt cgtcgagacc atcgacatga
     4981 gcgccgccac gcagtcgcgc ctcaacggca cggtctcctt caactaccgc cccatcatca
     5041 cgagcggcaa cacggcggcc atctacctgc accagaagct cgcctacaac cagagctact
     5101 acgtcacgat ggagccgggc gtcatccgcg acgcggcggg cgcgcccttc gtcggcttcg
     5161 ccgaaaacac ggtctggaac ttctcgacca agacgagcgg ccccgccgca ggctcggtcg
     5221 cgctcacggt cgcggcagac ggcaccggcg acttctcgac cgtgcagggc gccatcgact
     5281 tcgtccccgc gaacaacacg cggcgcgtcg tcatcaccgt caagaagggc acctacaccg
     5341 agatcgtcta cgtcaactcg aacaagccct tcatcaccgt gcgcggcgaa gaccgcgcct
     5401 tgagcatcgt cagttacgcc aacaacaaca acttcaatcc gacctcgacc accacgcgcg
     5461 cgatgttcgg cgtggatgcc ccggacttca cgctcgaaaa catcacgctc cacaacacga
     5521 cgccgcaggg cggctcgcag gccgaggcgt tccgcggcaa caacaaccgc atcgtgctga
     5581 accgcgtcaa cctcaagagc tttcaggaca cgctcctgct tcaaggccaa tcgaatcagg
     5641 gcggcttcgt caccgacagc tacatcgagg gcgacgtgga cttcatgtgg ggcggcggcc
     5701 cggtcttctt ccagaacacc gagctgaaga tgcttcgcac cgacgccttc tacatccagt
     5761 cccgcaacgt ctcgggccgc aacggcttcg tctacgtcaa ctgcaagttc acgcgcgccg
     5821 cgggcatcaa cggctctttc ctgaaccgca tcgaccccga cgacttcccc ttcagccagg
     5881 tcgtcatcat caactcgcag atggacggcg tccgcccaga cccgtggcag ttcaacaacc
     5941 ccacgaacgc ggtcaacgcg gccaactacc cgaacatccg catgtgggag tacaacagcc
     6001 gcgacctcag cggcaacctc gttgacgtga gccagcgcca ccccatctcg cgccagctca
     6061 ccgccgcaga ggccgcgcag tggagcgacc ccgcgttcgt gctcggcggc tgggcgccgc
     6121 agaccaagct caccgccgcc gtctcgctct cgaacctgac ccagaacttc aacggctcgc
     6181 ccgccgtcgt cacagctacg accgagccgg ctggcctgcc cgtcaccgtc acctacaacg
     6241 gctcgaccac gccgccttcc gccatcggct cctacgcggt cgtcgccatc gtcgatgacg
     6301 cgacctatca gggcacggcc aacggcaccc tcgtcatcga ccgcacgccc gtctccgtca
     6361 cgctctcgaa cctcgtccac acctacgacg gcacgccgaa gtctgcctcc gtcggcacca
     6421 acgtccccgg ccctgtcgtc tcagtcacct acaacggctc gaccgcgccg cccacggagg
     6481 tcggcaccta cagcgtcgtc gccgccatca acgaccccaa ctaccgcggc ggcacctcgg
     6541 ccacgatgac catctacgcg cccggcgcgc agcccctcaa agccttcccc ggcgcagagg
     6601 gcgcgggcga gctttcgcgc ggcggtcgcg gcggcgacgt gtaccacgtc accaatcttg
     6661 acgactccgg cgcgggttcc ctgcgcgaag gcatccgcac cgcgagcggc ccccgcacca
     6721 tcgtcttcga cgtgtcgggc acgatccatc tcaactctcg tctgaccatc aacaagccct
     6781 tcctcaccat cgccggccag accgcgcccg gcgacggcgt caccatcgcg ggctggccga
     6841 cggtcatcac gaacaccaac aacgtcgtcg tccgctacct gcgcttccgc gtcggcgacg
     6901 cgaactgccc cgtcgtgcag gacgacgcgc tctgggtgga caaggcgacc gacatcatcc
     6961 tcgaccacgt ctccgcctcc tggagcgtgg acgagacgct ctccgtcaca gagtccgacc
     7021 gcgtcaccgt ccagtggtct ttcatcaccg agagcatgaa gaactcctgc cacgagaagg
     7081 gcgcgcacgg ctacggctcg ctcatccgct tcggcaacgg cgtcgtcact tatcatcaca
     7141 acctctacgc gcaccacgac agccgcaacc cgcgcgtcgg cgaccagatc ggcctcgatt
     7201 tcgtcaacaa cgtcatctac aactggggcg gcgagagcgg ctacagcggc cccgccgagg
     7261 aaggcacgac gcgcgtcaac tacgtcggca actacctcgt cgccggcccg cagaccaccg
     7321 cctccaagct ccgcgccttc agcggcggga gcgcgaacac gcaaatctat cagtccaaca
     7381 acttcctcga cggcgacaag gacggcgcgc gcgacggcgc cgacaacggc tggacgatgt
     7441 tcgtcggctc ttacaccaag cgcgagccgg cgcgcttcga cttcccgcag gtcacgaccc
     7501 acgacgccgc gaccgcctac gagcgcgtgc tgggcaccgc cggccactcg cgctcgcgcg
     7561 actcggtgga cacgcgcgtc gtgggcgaag tgcgcggcga gggcggccac cacatcaact
     7621 cgcagaacga cgtgggcggc tggcccgtcc ttgactcgac ggcggccccc gccgacacgg
     7681 acggcgacgg catccccgac gagtgggaaa tcaatcacgg ccttaacccg gcggacgcgg
     7741 cggacggtcg cgccaccacc gcgagcggct acacgaacct cgaacgctac ctcaacgaca
     7801 tcgtgcccgt gcccggcgtc gatccctcga ccgacagcgc cgcgcccacg gccacggcca
     7861 cgctctcgca cgaggggaac gccgccggct ggcacaactc ggacgtgacc gtcacgctcg
     7921 cggcggcgga caacgacggc ggctcgggcc tgcgcgaaat cacctacagc gtcaacggcg
     7981 cgttcgccta cgcgaacacg tcgccgatca cgatacctgt caacatcgaa ggcacgacga
     8041 cgatcacctt ctacgccggc gacctcgcgg gcaacaccgg cgcggcgcag gtcttgaccg
     8101 tcaagctcga caagaccgcg cccgtcgtcg acaccacgcg cgcgcccgaa gccaacgcga
     8161 acggctggaa caacaccgat gtcgtcgccg gttacagcgc gacggacgcc gtctccggct
     8221 tcggcgacgg ccccgacgag agcggcgcct acaccttcag cagtgagggc gcgggccaga
     8281 gcttcacctt ccaggtgaca gacctcgcgg gcaacgccgc gagcgcgacc gtggccgacg
     8341 tgagcatcga caagaccgcg ccggtcgtcc atctcaggcg gcccgtcgaa ggcgccttct
     8401 acctgctcgg ccaggcggcg ctcgccgact acgactgcgc cgacaacctc tcgggcctcg
     8461 cgtcttgcgc gggcacggtc gcggcgaaca gcctgttcga cacggccacc gtcggcggca
     8521 agaccttcgc cgtcaacgcc gccgacctcg cgggcaacac cgccgcgcag gccgtcggct
     8581 acacggtcgg ctaccagctc aacctgctct acgacgcgaa caaggtgcac aggggcggca
     8641 gcaccgtccc cgtcaagctg caactcttcg acagcgcggg cgcgaacctc tcgtccgccg
     8701 cgaccgtgtt gcacgccgtc ggcgtctcgc tcgtcacccc cgagaccagc ggcacggcgg
     8761 cgaccgacgc cggcaacagc aaccccgact ccgcattccg cttcgaccct tccttaggcg
     8821 gcacgggcgg ctaccaattc aacctcaaga ccgacggcct cgcccccggc acctaccgcc
     8881 tctccttcac ggtcggcggc gacccgcacg tctacgcggc gcagttccag atcaggtaac
     8941 tcgccaaccc cgacgcgccg aaaagcgcgc gggctgaaaa gagtaaggcc acgactgtta
     9001 tcgccgtggc cttactctgt ttcacatgaa ctgaaatggc gcggggcgtc gccttcgtca
     9061 gcgcttcttg gctcccacga acggcctgta tggtctgacg cctgacttct tcgacgcacc
     9121 cttgcgcgct aatttacgca agccgccctg caccgagtac gatgggcgca tagaccgcat
     9181 cgcgcgtcgc ttgctcttct ttttcgcggg cttcgggttg agccgcgccg agagcttctt
     9241 gacctctccc ttgttgccga tctgttggta gagttcgagc gcgcgggtca gcagttcgcg
     9301 cgcctcgtcc ctttcgccgc ccgcctcttc tatgtccgcg aggttgacgt aggtgagggc
     9361 catgccgtcc ttgtggccga gggccttcgc catcgacagc gccgcctcgt acattcgttt
     9421 cgccttcctc aactcgccct gcgacacgtg gaggttgccg aggttgccgt gggcgcgctc
     9481 catgccggcc ttgctcccga gcgagcggga caacttcagc gctctggtgt aagcctcctc
     9541 ggcctccgcg aacttccgct gccgctcgct gacgatgccg aggccgaggt acgcgttcgc
     9601 catgccctcc ttttcgtcca acgcctcggc ca
//
LOCUS       DBNGOH010000959         4793 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_877354, whole genome shotgun sequence.
ACCESSION   DBNGOH010000959 DBNGOH010000000
VERSION     DBNGOH010000959.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4793)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4793)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4793
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_877354"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..475)
                     /locus_tag="AC159P_18655"
     CDS             complement(<1..475)
                     /locus_tag="AC159P_18655"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010154373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR3697519.1"
                     /translation="MSKETLHREVVIIGSGPAGLTAAIYAARADLKPLVVHGPQPGGQ
                     LTITTDVENYPGFSKGIQGPELMQEFEEQARRFGTEFLQAHITSVDLGRRPFTLHTDD
                     HTIKAETLIIASGASAKWLGVPGEAPAPEGLGGMGVSACATCDGFFFKGKPIVVVG"
     gene            647..1558
                     /locus_tag="AC159P_18660"
     CDS             647..1558
                     /locus_tag="AC159P_18660"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008011717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003857 - (3S)-3-hydroxyacyl-CoA
                     dehydrogenase (NAD+) activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HIR3697520.1"
                     /translation="MNTPTHSEVSNHPHTPVVPVERVAVLGAGTMGHGIAQAAAAAGY
                     AVTLRDITPELVARALRSIEANLNKGVERGKVTAEERARVLANVRGTTDFEETAASDL
                     FIEAVPERLELKRETLREVEGVAGTRAFIFASNTSSLSITEIAEGARHPERVVGMHFF
                     NPVHIMRLLEIVVGRETSAETVEAAREAGRRMRKEPITVRDVPGFASSRLGVAVGLEA
                     MRMLEEGVASAKDIDTAMELGYNHPMGPLKLSDLVGLDVRLNIADYLHRKLGSERFRA
                     PDILRRMVAAGKLGKKSGEGFYKWEQQ"
     gene            1662..2039
                     /locus_tag="AC159P_18665"
     CDS             1662..2039
                     /locus_tag="AC159P_18665"
                     /inference="COORDINATES: protein motif:HMM:NF018894.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HIR3697521.1"
                     /translation="MTKPFDGERRFALRMALRLPVVVSGRADDGSAWSEPTETDDIST
                     LGALFHLNQKVGRGDQLYIRAHRPDGSPIEATARVVRIAPAIYGTARVGVQIMEPADN
                     WLRLFVSWVADEQGSSSPTGEEQ"
     gene            2136..2918
                     /locus_tag="AC159P_18670"
     CDS             2136..2918
                     /locus_tag="AC159P_18670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019850525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR3697522.1"
                     /translation="MASYETLLVERRDRVAVITINRPQKLNALNIQTRQEGAAALDEL
                     RADEGVGVVVITGAGEKAFVAGADIAEFEGRTAVTQRDVMLGRSLFTAVDTFPKPIIA
                     MINGFCLGGGCELALACDLRIAGDNARFGQPEINLGIIPGGGGTQRLARLIGEGKAME
                     LILTGDMIDAQAAYNLGLVNMVVPAPDLEGKTMELAGRIAAKSPVALRMAKEAVKTAS
                     RANLDEGLRREVDLFAICFSSEDKDEGVRAFLEKRKPEFKGR"
     gene            2959..3198
                     /locus_tag="AC159P_18675"
     CDS             2959..3198
                     /locus_tag="AC159P_18675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697523.1"
                     /translation="MAAKNNHLVYVGLFMAAMGFVFAIDTVQWLASYDDYYAAVKTNA
                     HAGFTQKVRVVASVVLVAAGLLISIAAARGCKRLD"
     gene            complement(3366..4286)
                     /locus_tag="AC159P_18680"
     CDS             complement(3366..4286)
                     /locus_tag="AC159P_18680"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR3697524.1"
                     /translation="MNKSVAAQGGTAGEGAERAYLYMLAGSLAFATMGALSHLAGERV
                     DWRVVAVARTLVAFVLSASLTLSTGVRLVVLRPPVLWMRSVAGSVGVLCAFYALTHLP
                     VSTALTLSNTVPVWVTLLAWPALGQRPAASVWGAIAVGVCGIALVQRPELAADRLAGM
                     LAVGNALCTAVAMIGLNKLAGVDARAVVTHFSGVSTVFTLSVLLMSDGVDYGAVWSLT
                     TLGLLAGVGAAGTLGQLGMTKAFALGNPPRVSVVGLMQIVFALLFDLLLWRRRFDALT
                     VAGIFLVVAPSAWLMLHNPLKKGTPIHTTQ"
     gene            4504..4576
                     /locus_tag="AC159P_18685"
     tRNA            4504..4576
                     /locus_tag="AC159P_18685"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4537..4539,aa:Ala,seq:ggc)
     gene            complement(4649..4793)
                     /locus_tag="AC159P_18690"
                     /pseudo
     CDS             complement(4649..4793)
                     /locus_tag="AC159P_18690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004507327.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
BASE COUNT          848 a         1666 c         1532 g          747 t
ORIGIN      
        1 cgccgacgac gacgatgggc ttgcccttga agaagaagcc gtcgcacgtc gcgcacgccg
       61 agacgcccat gccgcccagg ccctcgggcg cgggggcctc gccggggacg cccagccact
      121 tcgccgacgc gcccgaggcg atgatgagcg tctcggcctt gatcgtgtgg tcgtctgtgt
      181 gaagcgtgaa cgggcggcga ccgagatcga cgctggtgat gtgtgcttgc agaaactccg
      241 tgccgaagcg gcgggcctgt tcctcgaact cctgcatcag ctccggcccc tggatgcctt
      301 tggagaagcc ggggtagttc tcgacatccg tggtaatggt gagctgcccg ccgggctgcg
      361 ggccgtggac gacgaggggc ttcaggtcgg cgcgcgcggc atagatggcg gcggtcaggc
      421 ccgccggccc cgagccgatg atgaccactt cgcggtgcag ggtttctttc gacatctgtc
      481 tccttcccgt ctctcgcgcc gccgccaccg gggcgaagcg cggcgcggcg cgttggcctt
      541 tttccttgcg cgtgtagtat aacgtctcgc gccggaagta gtcgaattta cgacctaacg
      601 acgccggagg ccgtgcgccc catgtgcctc gccgtcgccg aacctattga acactcccac
      661 gcacagcgaa gtcagtaatc atcctcacac gccggtcgtc ccggtcgagc gcgtcgccgt
      721 cctcggcgcg ggcacgatgg gccacggcat cgcgcaggcc gccgccgccg ccggctacgc
      781 cgtcaccctg cgcgacatca cgccggaact cgtcgcgcgc gcccttcgct ccatcgaagc
      841 gaacctgaac aaaggcgtcg agcgcggcaa agtcaccgca gaggagcgcg cccgcgtgct
      901 cgcgaacgtc cgcggcacga ccgacttcga ggagaccgcc gccagcgacc tcttcatcga
      961 ggccgtgccc gaacgccttg agctgaagcg cgagacgctc cgcgaagtgg agggggtcgc
     1021 gggcaccaga gccttcatct tcgcctcgaa cacctcttcg ctctcgatca ccgagatcgc
     1081 cgagggcgcg cgtcatcccg agcgcgtcgt cgggatgcac ttcttcaacc ccgtccacat
     1141 catgcgcctg ctggagatcg tcgtcgggcg cgagacctcc gccgagacgg tcgaggcggc
     1201 gcgcgaggcg ggccgccgga tgcgcaagga gccgatcacg gtgcgggacg tgccgggctt
     1261 cgcctcctcg cggttgggcg tcgcggtcgg cctcgaagcg atgcggatgc tcgaagaggg
     1321 cgtggccagt gcgaaagata tagatacggc gatggagttg ggctacaatc accccatggg
     1381 gccgctcaaa ctttccgact tagtcggcct cgacgtgcgc ctgaacatcg ccgactacct
     1441 gcaccgcaag ctcggaagcg agcgcttccg cgcacccgac atcctgcggc gcatggtcgc
     1501 cgccggcaag ctcggcaaga agagcggcga gggcttctac aaatgggaac agcagtaggc
     1561 agtaggcagt caaaacgtga ttgtcttacg ccaccgcctc ttcaacaatc aaccgctccg
     1621 ttccgaactg cctactgcct actgcctact gcctactgct tatgaccaag cctttcgacg
     1681 gggagagacg gttcgcgctg aggatggcgc tgcggctgcc ggtcgtcgtg tcgggccggg
     1741 ccgacgacgg ctcggcgtgg agcgagccaa ccgagacgga cgacatttcg acgctgggcg
     1801 cgctcttcca cttgaaccag aaggtggggc gcggcgacca gctctacatc cgcgcgcacc
     1861 gccccgacgg ctcgcccatc gaggccacgg cgcgggtcgt ccgcatcgcg cccgccatct
     1921 acggcacggc ccgcgtcggc gtccagatca tggagccggc ggacaactgg ctccgcctct
     1981 tcgtctcctg ggtcgccgac gagcaaggct cctcctcgcc caccggcgaa gaacaataaa
     2041 aaacgtaaac cgtgaaccgt aaaccgtgac aagtaagaac tgcttgcttc ttgtcacggt
     2101 ttacggttcg cggtttacgg ctctcggagg ttcgcatggc atcatacgag acacttcttg
     2161 tcgagcggcg cgaccgcgtc gccgtcatca cgatcaacag gccgcagaag ctgaacgcgc
     2221 tgaacatcca gacgcggcaa gagggcgcgg cggcgctgga cgagctgcgc gcggacgaag
     2281 gggtgggcgt cgtcgtcatc acgggcgcgg gcgagaaggc gttcgtcgcg ggcgccgaca
     2341 tcgcggagtt cgaggggcgc accgccgtca cgcagcgcga cgtgatgctc gggcgctcgc
     2401 tcttcaccgc ggtcgatacc ttcccgaagc cgatcatcgc gatgatcaac ggcttctgtc
     2461 tgggcggcgg ctgcgagctg gcgctcgcgt gcgacctgcg catcgcgggc gacaacgcgc
     2521 gcttcggcca gcccgaaatc aacctcggca tcatccccgg cggcggcggc acgcagcgcc
     2581 tcgccagact catcggcgag ggcaaggcga tggagctgat cctgacgggc gacatgatcg
     2641 acgcgcaggc cgcctacaac ctcggcctcg tcaacatggt cgtgcccgcg cctgacctcg
     2701 aaggcaagac gatggagctg gcgggccgca tcgccgcgaa aagcccggtc gcgctccgca
     2761 tggcgaagga ggccgtgaag acggcctcgc gcgcgaacct cgacgaagga ctaaggcgcg
     2821 aggtcgatct ctttgccatc tgcttttcga gcgaggacaa ggacgagggc gtccgcgcct
     2881 tcctcgaaaa gcgcaagccc gagttcaagg gccgctgacg gcgccggggc gagaggtgaa
     2941 gggagggggc cgcaggccgt ggccgcgaag aacaaccatc tcgtctacgt cggcctcttc
     3001 atggcggcga tgggtttcgt cttcgccatc gacaccgtgc agtggctggc ttcttacgac
     3061 gactattacg ccgccgtcaa aaccaacgct cacgcgggct tcacgcagaa ggtgcgcgtc
     3121 gtcgcctccg tcgtgctcgt cgccgccggc ctcctcatct cgatagccgc ggcgcgcggc
     3181 tgcaaacggc tcgactgaga ttcgccgcgc gtcgctgccc gtctaaggaa agtgagcggg
     3241 gagcaataaa cagcgggcag cgggcaacag gcagtcaata cagaagggtg aagaggcttg
     3301 agatgcgatg tctcaagcct cttgcttcgt ccgtctttac tgcctgctgc ctgctgcccg
     3361 ctgtttcact gcgtggtgtg aatcggcgtg cccttcttga gcgggttgtg gagcatcagc
     3421 catgcggaag gcgcgacgac gaggaagatg cccgcgaccg tcagcgcgtc gaaccggcgg
     3481 cgccacagca gcaggtcgaa gagcagcgcg aagacgatct gcatcaggcc cacgaccgac
     3541 acccgcggcg ggttgccgag cgcgaacgcc ttcgtcatcc ccaactgccc gagcgtcccc
     3601 gccgcgccga cgcccgccag cagtccgagc gtcgtcaaac tccataccgc gccgtagtcc
     3661 accccgtccg acatcaagag caccgagagc gtgaacacgg tcgagacgcc cgagaagtgc
     3721 gtcaccacgg cgcgcgcgtc cacgcccgcg agcttattca aaccgatcat cgccaccgcc
     3781 gtgcacaggg cgttgcccac ggccagcatc cccgccagcc tgtccgccgc gagctccggc
     3841 ctctgcacga gcgcgatgcc gcacaccccc accgcgatgg ccccccacac cgacgccgcc
     3901 ggcctctgcc cgagcgcggg ccacgccagc aacgtcaccc acaccggcac cgtgttcgac
     3961 agagtcagcg ccgtcgagac cggcaaatgc gtcagcgcgt agaacgcgca cagcacgccc
     4021 acgctccccg ccacgctccg catccacaac accgggggcc gcagcacgac gagccgcacg
     4081 cccgtcgaga gcgtcaggct ggccgacagt acgaacgcca ccagcgtgcg cgccaccgcc
     4141 accacccgcc agtccacgcg ctcgcccgcc agatggctca atgcgcccat cgtggcgaac
     4201 gccagcgacc ccgcaagcat gtagaggtac gcccgctcgg cgccctcgcc cgcggtgccc
     4261 ccctgcgcgg caacggattt attcatcgaa acggaacgtc cgcggatttt ctcatcgaag
     4321 acgggagagc ctaacacgcc ccctccgacg ctgtcagccc gcggcgaccg cgagacgcac
     4381 gcacgcccct tctccgacgc ccctccacac ttcctccgcg tttgcccatt ggcgcgcaac
     4441 tgtgctaact ttttttcgcc cgcccgaagg agctttcgcc gacccgcggg ccgttcgcaa
     4501 caaggggccg tagctcagct gggagagcgc ttgaatggca ttcaagaggt caggagttcg
     4561 atcctcctcg gctccattaa cttagataga taaaggactt atagctttca ggccgaatcg
     4621 aaagaggagg tttgagcgca tgagtcccga agcgggtctg cccttcgggg acgatcttcg
     4681 gctcgaagct ggagttgcgg tcgcgcggga cttcgatagg gaggttgccg aactccccct
     4741 tgagcgtctt cttggacttg ccgttgcggg agttgccgga gttatcgccc gcc
//
LOCUS       DBNGOH010000960         8990 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3261217, whole genome shotgun sequence.
ACCESSION   DBNGOH010000960 DBNGOH010000000
VERSION     DBNGOH010000960.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8990)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8990)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8990
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3261217"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1594
                     /locus_tag="AC159P_18695"
     CDS             <1..1594
                     /locus_tag="AC159P_18695"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016863646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HIR3697525.1"
                     /translation="RGGESSVEAFGPSFGVPEENVQVISPYVGGAFGSSLRPNYYPAL
                     TAMAARELKRPVKVVYTRTQMYTGHGYRPYTIQKIALGAEASGKLSAMIHEAVHNTST
                     FEEFNDNTTGFTRQVYACPNLFAPTKITDTDLNTPTWMRAPGAVSGMFALECAMDELA
                     YALKIDPLELRLINYAETDPESGKPWSSKALRECYRLGAEKFGWKQRKMEPRSMRDGR
                     LLVGWGTATGIWGAFQMPAAARIIFKADGTAHVASATSDIGPGTYTMATMIAAEYLGL
                     TPERVKFELGDTNLPRAPSQGGSWTTASVGSAIHGAALAVTAKLLALANREANSPLKA
                     AAAADVEMLDGRLRLKADPSKSVVISDLMRRNGLAEITETFESKPSEERRKYATMAHG
                     AQFVEVKVDPDLGNVRVTRVIEVTACGKIINPKASHSQEIGGVVWGIGMALQEATEID
                     HRYGRIMNPNLQHYHVPVNADVHEVETLFVEEDDKVVNPLGVKGMGELGMVGIPAAIA
                     NAVFHATGKRVRELPITPDKLL"
     gene            1609..2826
                     /locus_tag="AC159P_18700"
     CDS             1609..2826
                     /locus_tag="AC159P_18700"
                     /inference="COORDINATES: protein motif:HMM:NF024869.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HIR3697526.1"
                     /translation="MNEVRALVEAFDEANERGAQCALATVVSVEGSSYRRPGARMLVC
                     EGGTSTGTISAGCLEGDVIEHAKHVIRRGAAKLVEYDTASTSDEMAWGLGLGCNGIVR
                     VLVEPLVPGSPYVEALRRSCEAQADIAPVSVVTVFQHTPSESRRAEGRVEIGARLFIN
                     DDGEVSREKLSDRVAAMLEGEMRELSRGGETSGARVCDVDGGTVKVFIETLLPPVPLV
                     IFGAGHDALPIVELARGLGWQTEVVDPQARPVSRSRFAMADRVTLSRPEDVGAQVSIT
                     PRTLTLLMSHNYSHDLELLKFLLASPARYIGVMGPRKRTERMLSELAAGEDAYRLEEA
                     GLSRFHSPAGLDIGANAPAEIALSIVAEMRAVLDERRGGMLRERRGSIHGGTGVGERV
                     PLREEQTRSVAVA"
     gene            2861..3487
                     /locus_tag="AC159P_18705"
     CDS             2861..3487
                     /locus_tag="AC159P_18705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017721223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HIR3697527.1"
                     /translation="MVESDSVGVGAVILAAGSSSRMGSPKQTLQYRGESLLRRAALAA
                     LGAGLRPVIVVTGAGAELSRRELDGLDVREVLNTRWETGMASSIRAGVEGLLNAAPGA
                     DAAVLMLCDQPHLTSEVISGLVSAHNATGSPVVASAYGGSFGVPALFGRTLFAELARL
                     EGATGAKQVIKRHAAEAHFIPFPGGEVDVDTPDDFSRLTAGDVGQEQA"
     gene            3681..4676
                     /locus_tag="AC159P_18710"
     CDS             3681..4676
                     /locus_tag="AC159P_18710"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015186398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR3697528.1"
                     /translation="MQKRTLGNSDLEVSALGLGCMGLSFGYGPATDKQEAVALIRAAV
                     ERGVTFFDTAEVYGPFTNEELVGEALAPFRGQVVIATKFGFKPASDGEGRWSELDSRP
                     EHVGDAVEGSLKRLGVETIDLLYQHRVDPEVPIEDVAGAVKDLIHEGKVKHFGLSEAG
                     VQTIRRAHAVQPVTALQSEYSLWWRSPEEELIPTLEELGIGFVPFSPLGKGFLTGKID
                     ENTTFDSTDFRNIVPRFTPEARKANQTVVDLLGRIAAQKNATPAQIALAWLLAQKPWI
                     APIPGTTKLHRLEENMGAAEVELTPDDLREIDGASSEIKVQGGRYPAHLEKMTGR"
     gene            4776..4973
                     /locus_tag="AC159P_18715"
     CDS             4776..4973
                     /locus_tag="AC159P_18715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697529.1"
                     /translation="MNDTRSSTTSSRAVVLANILVSYTMIVLDISVVITALPKIRDGL
                     GFSEARLSSTARRSFYNLQQR"
     gene            4992..6026
                     /locus_tag="AC159P_18720"
     CDS             4992..6026
                     /locus_tag="AC159P_18720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HIR3697530.1"
                     /translation="MRATIMYGAGDVRVETVPDARIIAPTDALVRVTRACICGSDLWP
                     YKSMAPSETGNRMGHEFIGLVEAVGAEVRTVKRGDLVVAPFAWSDGTCVFCREGLQTS
                     CLHGGWWGGTDLDGGQGEAVRVPQADGTLVVLPVGRDDALMPSLLTLSDVMGTGHHAA
                     LAAKVGPGKSVAVVGDGAVGLCGVIAARRLGAEQIIIMGRHPDRIALARDFGATDVVS
                     ERGDEAIERVRELTGGLGAHSVLECVGYEEATLTALSIARPGGAVGRVGVPQDEKIPA
                     AGPTFFGNVTIAGGPAPVRAYIEELLPDVLEGRIEPGRVFDRVTDLDGVPDGYRAMDE
                     REAIKVMIGF"
     gene            6088..6897
                     /locus_tag="AC159P_18725"
     CDS             6088..6897
                     /locus_tag="AC159P_18725"
                     /EC_number="4.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(R)-mandelonitrile lyase"
                     /protein_id="HIR3697531.1"
                     /translation="MKLLAATVMSLSLLASASAQDSQPIRITRSGSQPSRQGPAENFT
                     GSVRVDTPFQANAPARTSGALVTFEPGARSAWHSHPLGQTLIVTTGVGRVQRWGDPVD
                     EIRPGDVVWIPPGQKHWHGASPDAAMSHIAIVEQLDGKSTDWMEKVSDAQYGAPVRAQ
                     PPTTAVQPTAGKQLMGDIAPKLAELTDDVLFADVWERPGLSKRDRSLATVAALIAMNR
                     PDQLRSHLARARENGVTEQELIEAITHLAFYAGWPSAVTAVGVAREVFGKK"
     gene            6935..7513
                     /locus_tag="AC159P_18730"
     CDS             6935..7513
                     /locus_tag="AC159P_18730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006341125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HIR3697532.1"
                     /translation="MKNVIVLGAGGNIAKHVIDILVKRDDVNLTLFLRDRSRLRNKDV
                     SGCRIVEGDVLDFDRLKEAVAGQDVAYANLAGDLEAMAKNIVRAMDETGVKRLIFISS
                     IGIYDTTLRPVLKPYRKAADVIEASNLEYTVLRPTWFTNADEVDYETTRKGEPEKGSV
                     ISRKSLATLITKMIQSPDGYARENLGVNKPNS"
     gene            7555..8067
                     /locus_tag="AC159P_18735"
     CDS             7555..8067
                     /locus_tag="AC159P_18735"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016556250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3697533.1"
                     /translation="MMSPDYQTGPVVRRATAADLPGIGRLGALLVEEHYAFDPKRFLA
                     ARPGTPAGYASFMSAQLEDPDVTVLVADDKATVVGYAYAAVEGYDYMALRGPAGVLHD
                     VIVDPEYRGRGVGRLLLDAALAFLRSRGVPRVVLSTAARNEAAQRLFASVGFRRTMIE
                     MTRELDDSAS"
     gene            8156..8990
                     /locus_tag="AC159P_18740"
                     /pseudo
     CDS             8156..8990
                     /locus_tag="AC159P_18740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002620863.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter domain-containing
                     protein"
BASE COUNT         1602 a         2867 c         3059 g         1462 t
ORIGIN      
        1 gcgcggcggt gagtcctccg tagaagcctt cggcccgagc ttcggcgtcc ccgaggagaa
       61 cgtgcaggtc atttcgccct atgtcggggg cgcgttcgga tcgtcgctac ggccgaacta
      121 ttacccggca ctgacagcca tggcggcgcg cgaactcaag cgtcccgtca aggtcgtcta
      181 cacgcggacg cagatgtata ccggccacgg ctaccgcccc tacacgatcc agaagatcgc
      241 gctcggcgcc gaagcgtcgg ggaagctgtc cgcgatgatc cacgaggccg tgcacaacac
      301 ctctaccttc gaggagttca acgacaacac caccgggttc acgcgccagg tctacgcctg
      361 cccgaacctc ttcgcgccga ctaaaatcac cgacaccgac ctcaacacac cgacctggat
      421 gcgcgcgccc ggggcggtga gcgggatgtt cgcgctcgaa tgcgcgatgg acgaattggc
      481 ctacgcgctg aagatcgacc cgctggagct gcggctcatc aactacgccg agaccgaccc
      541 cgagagcggc aagccgtggt cgagtaaggc gctccgggag tgctaccggc tcggggcgga
      601 gaagttcggc tggaagcagc gcaagatgga gccgcgctcg atgcgcgacg ggcggctgct
      661 ggtcggctgg ggcacggcca ccggcatctg gggcgcattc cagatgcctg ctgccgcccg
      721 aatcattttc aaggcggacg ggacggcgca cgtcgccagc gcgacgagcg acatcggccc
      781 cggcacctac acgatggcca caatgatcgc cgccgagtat ctgggcctca cgcccgagcg
      841 tgtgaagttc gagttgggcg acacgaatct cccgcgcgcc ccttcgcagg gcggctcgtg
      901 gacgacggcg agcgtcggct ccgccatcca cggcgcggcg ctggcggtca ctgctaagct
      961 gctcgcgctc gcgaaccggg aggcgaactc gccgctgaag gcggcggcag ccgccgacgt
     1021 tgagatgctc gacggcaggc tccgtctcaa ggccgacccg tcgaagtccg tcgtcatctc
     1081 cgacctcatg cggcgcaatg gcctcgcaga gatcacggag acgttcgagt caaaaccctc
     1141 tgaggagcgc cggaagtacg cgacgatggc gcacggcgcg cagttcgtcg aggtcaaggt
     1201 tgatcctgac ctcgggaacg tccgcgtcac gcgcgtcatt gaagtgacgg cttgcggcaa
     1261 gatcatcaac ccgaaggctt cgcacagcca ggagatcggc ggcgtcgtct ggggcatcgg
     1321 catggccttg caggaggcga ccgagatcga ccaccgctac ggccgcatca tgaacccgaa
     1381 cctccagcac taccacgtcc ccgtcaacgc cgacgtgcac gaggtcgaga cgctgttcgt
     1441 cgaggaggac gacaaggttg tgaacccgct cggcgtcaag ggcatgggcg agctcgggat
     1501 ggtcggcatc ccggcggcca tcgccaacgc cgtcttccac gccaccggca agcgcgtcag
     1561 ggaattgccg atcacgccgg acaagttgct gtaaacaggg gagagcttat gaacgaggtg
     1621 agggctttag tcgaagcgtt cgatgaggcg aacgagaggg gcgcgcagtg cgccctggcg
     1681 acggtcgtga gcgtcgaagg ttcttcttac cgacgtccgg gcgcgcggat gctggtctgc
     1741 gaaggtggga ccagtaccgg tacgatcagc gccggctgcc tggagggcga cgtcatcgag
     1801 catgcgaagc acgtcatacg gaggggtgcg gcgaagctcg tcgaatacga cactgcctcg
     1861 acgagcgacg agatggcctg ggggcttggc ctcggctgca acggcatcgt gcgcgtgctg
     1921 gtagagccgc tcgtaccagg gtcgccctac gtggaggcgc tgagacgttc ctgcgaagcg
     1981 caagccgaca tcgcgcccgt gtcggtcgtg accgtgtttc aacatacgcc gtcggagtcg
     2041 aggcgggccg aggggcgcgt cgaaataggg gcacgcctct tcatcaacga cgatggtgaa
     2101 gtcagccgcg agaagttgag cgaccgtgtg gccgctatgc tcgaaggtga gatgcgcgag
     2161 ttgtcgcgcg gaggggaaac atcgggcgcc cgcgtctgtg acgtggatgg tggcaccgtg
     2221 aaagtattca tcgaaacgct cctgcctccc gtcccgctcg tcatcttcgg ggcgggacat
     2281 gacgcactgc cgatcgtcga actcgcgcgc gggttgggat ggcagacgga ggtcgtcgat
     2341 ccgcaagccc gccccgtcag tcgttcacgg tttgcgatgg ccgacagggt cacactctcg
     2401 cgccccgaag acgtcggcgc gcaggtgagc attacgccgc ggacactgac gctgctgatg
     2461 tcgcacaact actcgcacga cctcgaactg ctcaaattcc tgctcgcgtc ccccgcccgc
     2521 tacatcgggg tcatgggccc acgcaaacgg accgaacgga tgctgagtga gttggcggcg
     2581 ggtgaagacg cgtaccgcct cgaagaggct ggcctgtcac gctttcattc cccggctggt
     2641 ctggatatag gggcgaacgc tcccgcagag atcgcgctgt cgatcgtcgc cgaaatgcgc
     2701 gcggtcttag acgagcggcg cggcgggatg ttgcgcgagc gccggggctc cattcacggc
     2761 ggcacgggtg tcggcgagcg cgtccccttg agagaggaac agacccggtc tgtggcagtg
     2821 gcgtgagggg cgaaggctga ccggggaggg catttccagg gtggtcgaaa gcgactcggt
     2881 gggcgtcggc gctgtaatcc tggccgcggg ttcgtcctcc cgcatgggca gcccgaagca
     2941 gaccttacaa taccggggcg agagtctgct caggcgcgct gccctcgccg cgctgggcgc
     3001 ggggctccgc cccgtcatcg tggtgacggg cgccggcgcc gagctgtcga ggcgcgagct
     3061 ggacgggctc gacgtgcggg aagtgttgaa tacccgctgg gagaccggga tggcttcgtc
     3121 catacgggcg ggcgtcgaag gccttctcaa cgcggccccg ggcgcggacg ccgccgtcct
     3181 catgctgtgc gaccagcctc acctcacctc cgaagtcatc tccggcctcg tctcggcgca
     3241 caacgccacg ggaagccccg tcgtcgcctc ggcgtacggc gggagcttcg gcgtgccggc
     3301 gttattcggc aggacgctct tcgccgagct ggcgcggctc gaaggggcga ccggcgctaa
     3361 gcaggttatc aagaggcacg ctgcggaggc gcacttcata cccttcccgg gtggcgaggt
     3421 ggacgtggac acgccggacg atttctcgcg cctgaccgcc ggggacgtcg gacaggaaca
     3481 ggcttgacgc agtcctgacg acagagcaat cgtatcgcgg tgcgacattc gcgggagttg
     3541 tagcgaccgt cgtcctcccg cgcctcgggt ttcgcggttt agagcaacat cgccacagtc
     3601 cgagcaagag cgcgaacgac agccgcgcgg gcgcggggta gattggcctc acggtttaac
     3661 tgagaggggt gaattagaaa atgcagaaac gcacactcgg gaatagtgat ttggaagtct
     3721 cggccctcgg actcggctgc atgggactca gcttcggcta cggcccggcc acggacaagc
     3781 aggaggcggt tgcactgatc cgtgcggccg tcgagcgagg cgtcacgttc ttcgacaccg
     3841 ccgaagtcta cggcccgttc acgaacgagg aactcgtggg cgaggccctc gcccccttcc
     3901 gcggtcaggt ggtgattgcc accaagttcg ggttcaagcc cgcttccgac ggtgagggga
     3961 ggtggagtga gctggatagc cggccggagc acgtcgggga tgccgtcgag ggctcgctca
     4021 agaggctcgg agtcgagacc atcgacctgc tctatcaaca ccgcgttgac cccgaagtgc
     4081 ccatcgaaga cgtggcgggc gcggtgaaag acttgattca cgaaggcaag gtcaagcact
     4141 tcggactctc cgaagcgggc gtgcagacca tccgccgcgc gcacgccgtc caacccgtga
     4201 cggcgcttca gagcgaatac tcattgtggt ggagaagccc cgaagaggaa ttgataccga
     4261 cactcgaaga actcggcatc ggatttgtcc cgttcagccc gctggggaag ggtttcctca
     4321 cggggaagat cgacgagaac acgacgttcg acagcaccga cttccgcaac atcgtcccac
     4381 ggtttacgcc cgaggcgcgg aaggcgaacc agaccgtggt ggacttgctc ggcaggatcg
     4441 ccgcgcagaa gaacgccacg ccggctcaga tagcgttggc ttggctgctc gcacagaagc
     4501 cgtggatcgc gccgattccc ggcaccacga agctgcaccg cctggaggag aacatggggg
     4561 cggctgaagt cgaactgact cccgacgatc tacgcgagat cgacggcgct tcctcggaga
     4621 tcaaggtgca agggggacgg taccctgcac acctggagaa gatgaccggt cgctgaacgg
     4681 gccgggttaa gggtcgaaga gaagtaaccc gactcatttt tcatgcacga gcataaaccc
     4741 atatatgtcg cgaaggtctt gcgggggata gggcgatgaa cgacacgcgt tcgagcacta
     4801 cgtcgagcag ggcggtcgtg ctcgcgaaca tcctcgtgag ttacacgatg atcgtgctcg
     4861 acatctccgt cgtcatcacc gcgttgccga agatccgcga tggcctcggc ttctcggagg
     4921 cacggctctc aagcaccgcg cggcgtagtt tttacaacct ccagcagaga tgaacgaaag
     4981 gtagattaca catgcgagca actatcatgt atggcgccgg cgacgttcgt gtcgagaccg
     5041 tcccggacgc acgcatcatc gcaccgaccg acgcgctcgt ccgcgtcacg cgcgcctgca
     5101 tctgcggcag cgacctctgg ccgtacaagt cgatggcgcc gagcgagacg gggaaccgga
     5161 tgggccacga gttcatcggc ctcgtcgagg ctgtcggcgc cgaggtccgc acggtcaagc
     5221 ggggcgacct cgtcgtcgca ccgttcgcat ggtcggacgg cacctgcgtc ttctgccggg
     5281 agggcttgca gacttcgtgc cttcacggcg ggtggtgggg tggcacagac ctcgacggcg
     5341 gccagggcga ggcggtgcgc gtcccgcagg cggacgggac gctcgtcgtc ctcccggtgg
     5401 gccgcgacga cgcgctgatg ccgtcgctgc tcacgctctc ggacgtgatg ggcactggcc
     5461 accacgcggc cctcgccgcg aaggtcggcc ccggaaagtc ggtggccgtc gttggggacg
     5521 gcgcggtcgg cctgtgcgga gtcatcgccg cccggcggct cggcgccgaa cagatcatca
     5581 tcatgggccg ccacccggac cgcatcgcgc tggcacgaga cttcggcgcg acggacgtgg
     5641 tgagcgagcg cggagacgag gccatcgagc gcgtgcgcga gctgaccggc ggcctcggcg
     5701 cccactccgt cctcgagtgc gtcggttacg aagaggcgac gctcacggcg ctgagcatcg
     5761 cgcgaccggg cggcgcggtc ggacgcgtcg gcgtcccgca agacgagaag attccggcgg
     5821 cggggccgac cttcttcggc aacgtcacca tcgccggcgg cccggcccct gtgcgcgcct
     5881 acatcgagga gctgctgccc gacgtgctcg aaggcaggat cgagccgggg cgcgtgttcg
     5941 accgcgtcac ggacctcgac ggcgtgccgg acggctacag ggcgatggac gagcgcgagg
     6001 cgatcaaggt catgatcggg ttctgaaggg aagaaagcct tcagccacaa tgttctgatt
     6061 gttattcact gaatcgaaag gtacaaaatg aaactgcttg ccgcgacggt catgtcgctt
     6121 tctctgctcg cctcggcgtc agcccaggat tcgcagccga tcagaatcac gcggagcggc
     6181 tcgcagccat cacggcaggg gcctgccgaa aacttcaccg ggtcggtaag ggtcgatact
     6241 ccgttccaag cgaacgcccc ggcgcgcacg tccggcgccc tcgtcacgtt cgagccgggc
     6301 gcgcgctccg cgtggcactc gcacccgctg gggcagacgt tgatcgtgac gacgggcgtc
     6361 ggacgtgtgc agcgctgggg tgatcctgtc gatgagatca ggccggggga tgtcgtctgg
     6421 ataccgcccg gtcagaaaca ctggcacggg gcctcgccgg acgcggccat gtcgcacatc
     6481 gccatcgtgg aacagctcga cggcaagtct accgactgga tggagaaggt gtctgatgcg
     6541 caatacggcg cgccggttcg ggcgcagccg cccacgaccg ccgtacagcc taccgcgggt
     6601 aaacagttaa tgggcgacat cgcccccaag ctggcggagc tgaccgacga cgtgctgttt
     6661 gcagacgtct gggaacgtcc cggcttatcg aagcgcgacc ggagcctggc gacggtggcg
     6721 gcgctgatcg ccatgaatcg tcccgatcag cttcgctcgc atctcgccag agcgcgcgag
     6781 aacggcgtga cggagcaaga actaatcgag gccatcaccc atctggcgtt ctacgccggg
     6841 tggccgagcg ccgtcacggc agtcggggtg gcgagagaag tcttcggcaa gaaatgactc
     6901 gacagcgccg ttgaccgctc acagagaatg acctatgaag aacgtaattg tgctgggcgc
     6961 cggcggtaac atcgccaaac acgtcatcga cattctggtg aagagggatg acgtcaacct
     7021 gaccctgttc ttacgtgaca gaagcaggtt gagaaacaag gacgtttccg gatgccggat
     7081 tgtcgaaggc gacgttctgg atttcgaccg actgaaagag gccgtggcgg gacaggacgt
     7141 ggcttacgcc aaccttgcgg gagaccttga ggcgatggcg aaaaacatcg tgagagcgat
     7201 ggacgagacg ggggttaaaa gactcatctt catcagctcg ataggcatct acgacaccac
     7261 actcaggccc gtcttgaagc cctaccgcaa agccgcagac gtgatagaag cgtcgaacct
     7321 cgaatacact gttttgaggc cgacttggtt caccaacgcg gacgaggtgg actacgagac
     7381 gaccagaaaa ggcgagcccg aaaaaggctc ggtgatttct cggaaaagcc tggcgacgtt
     7441 gatcacgaag atgatccaat cccccgacgg atacgcccgc gaaaacctgg gcgtaaacaa
     7501 accgaactct tgatcgcgca gaaaaactac ggttcacaaa aacgtgagcc tcgtatgatg
     7561 tcaccagatt accagaccgg gcccgtcgtc cgacgcgcga cggctgccga ccttccgggg
     7621 atcggccgcc tcggcgcgct cctcgtagag gagcattacg ccttcgaccc taagcgcttc
     7681 ctggccgcga ggccggggac gccggcgggc tacgcgtcct tcatgagcgc gcagctcgaa
     7741 gacccggacg taaccgtcct cgtcgccgat gacaaggcga ctgtggtcgg ctacgcgtat
     7801 gccgccgtcg aggggtacga ctacatggcg ctgcgcgggc cggcgggcgt cctgcacgac
     7861 gtcatcgttg acccggagta tcgcggtcga ggcgtcggcc ggctactcct cgacgccgcg
     7921 ctggcgttcc tcaggtcgcg tggagtgccc cgcgtggtgc tctcgaccgc cgcgcggaac
     7981 gaggcggcgc agcggctctt cgcgagcgtg gggttccgcc ggacgatgat cgagatgaca
     8041 cgcgagctgg acgactctgc gtcctgacct tcggggccgc gctccggcgc gcggcagatg
     8101 gcgccgttga accggtgggt gtgacgatcg attcacccgg gggtctggag ggccgatgga
     8161 agttttcgag atcgtcgttg cgatgctgct gggcggcgcc gccctcgccg ccgtggcgcg
     8221 tcgcgtcggc gccccgtacc cggcgctggt ggcgctcgcc ggcgccgcgc tcgccctcgt
     8281 ccccggcgtg ccgacgctcg tcctcgaccc cgagttggcg ctcgcgctct tcgtcgcgcc
     8341 cgtcctcgtg gacgccgcct tcgacgcctc accgcgcgac ctgcgcgcca actggcgctc
     8401 tgtagccagc ctcgccctcg gggccgtcgc tctcacagtt atagtcgtgg cgctcgtggc
     8461 taaggcgctt gtccccgaca tgccttgggg cgctgccgtc gccctcggcg ccatcgtcgc
     8521 gccgccggac gcggcagccc gccaccaccg tgctcaaggc gctgcgcccg ccgcaccggc
     8581 tgctcgtgat cctggaaggg gagagccttt tcaacgacgc gagcgcgctg ctcatctacc
     8641 gcctcgcggt cggcgccacg gtcaccggct tcctttcggg gtggagcgtg ctgccgacac
     8701 tgctcatagt caccgtcggc agcgtcgtcc tcgccctcgt gcttgcgcgt ctcagcctcg
     8761 ccgtgaacgc gcgcatcagc gacgtctcga ccgcggtcgt cgtgcagttc tgcacgacat
     8821 tcggcgtctg ggtgctggcc gagcggctgc acctgtcggg catcatcacg atggtggtct
     8881 tcgcgatggc ggcggcgagg cgcgcgcccg cgatcatgcc ggcgcgcatc cgtatcccgt
     8941 cgtgggcggt gtgggaggtc gcggtcttcg tcctcaacgt gctcgcgttc
//
LOCUS       DBNGOH010000961         5368 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2894181, whole genome shotgun sequence.
ACCESSION   DBNGOH010000961 DBNGOH010000000
VERSION     DBNGOH010000961.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5368)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5368)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5368
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2894181"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..633
                     /locus_tag="AC159P_18745"
     CDS             <1..633
                     /locus_tag="AC159P_18745"
                     /inference="COORDINATES: protein motif:HMM:NF016176.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HIR3697534.1"
                     /translation="PLNKRSMTAVLFAVLLAVAPAYSAVTKYEIDTAHSNVGFSIPIL
                     GGLSSVRGKFTDFKVEITYDDKDVTKSSVNAVIKAASIDTGIERRDAHLKNADFFDVE
                     KYPEITFQSKHVEKKGKDFVAHGTFTMHGVSKEIALPFTLNGVQPAKDGKTVLGVTAR
                     TTLNRKDFGVSFARPDNPNFLGDNVEIELSLVTRAAAPAGTAAAPAPSGK"
     gene            705..1694
                     /gene="hprK"
                     /locus_tag="AC159P_18750"
     CDS             705..1694
                     /gene="hprK"
                     /locus_tag="AC159P_18750"
                     /inference="COORDINATES: protein motif:HMM:TIGR00679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HIR3697535.1"
                     /translation="MSDFVAHAPPHLGLSFLAGERGASSRLLDVPRIQKLGLALAGFA
                     HYVHAGRVQVVGQSEIAFLTQLTAERRQEAIRNVEFGKISCVLVTKGLVPPAEFVAAC
                     EEASLPLVQTPLVSSVAINAVTEYLVEALAPRETRHGVLLDLYGLGVLLEGKSGVGKS
                     ECALDLVVRGHRLVSDDVIEVKRTGPEQLVGSAPELLRELMEIRGLGILNVRDLFGVS
                     SISGPKQIGLTIRLVRWEETEEVDRLGLEARTTEILGVAVPYFLLPVSPGRNLSTLVE
                     TAVRVHLLRVRGYNAAGDFAARHAELIEGDFRRGEGAASSSSSPGEDGTGDVI"
     gene            1684..2670
                     /gene="rapZ"
                     /locus_tag="AC159P_18755"
     CDS             1684..2670
                     /gene="rapZ"
                     /locus_tag="AC159P_18755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012524233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HIR3697536.1"
                     /translation="MSSKKSGAERGQGSAHAPDIVIITGLSGSGMSSATNAFEDLGYF
                     CVDNLPLTLLPTFARLVRPEDRHAQHIERAALVIDIREGRFLREFPARLKELRASGKD
                     VHVVFLEASDDALVRRVSETRRPHPADDGGGLGEAIRGERAAMEDVRALADQVIDTSE
                     HTVHTLRRLLLERFSPDADGAEMRVQVISFGYKHGFPRDIDLMFDVRHLPNPHFVPEL
                     RDFSGHDPRVVKFLTASDEVKETIRRFAELADYLLPLYQREGKSYLTIGIGCTGGRHR
                     SVMVANALARHLRRGGFDATAVHRDVSKTKRQRGAAGKSKAKGGTREAGKVR"
     gene            2720..3181
                     /locus_tag="AC159P_18760"
     CDS             2720..3181
                     /locus_tag="AC159P_18760"
                     /inference="COORDINATES: protein motif:HMM:NF015567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HIR3697537.1"
                     /translation="MSKDVNEESEKGAAARRVGGVVVTHGQVATELLAAAETIIGAIK
                     HLTAVSIGWHDDVDAASAEVERAIERVSEGHGVLILTDMFGGTPTNIASMFLKKGEVE
                     VVTGVNLPMVIKLAGQAGGESLDEVAQRVRDQGREGIYLAGELLAGGGKKA"
     gene            3181..3459
                     /locus_tag="AC159P_18765"
     CDS             3181..3459
                     /locus_tag="AC159P_18765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-carbohydrate
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HIR3697538.1"
                     /translation="MVERSVRVVNRLGLHARAAARLVRTASAFRSAVRLERADRSATA
                     EAKSILSVLMLAASRGTELRAVAEGPDEREALDAVCELIEGGFGEQPE"
     gene            3456..5258
                     /gene="ptsP"
                     /locus_tag="AC159P_18770"
     CDS             3456..5258
                     /gene="ptsP"
                     /locus_tag="AC159P_18770"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HIR3697539.1"
                     /translation="MKHPKGKGEARWRGTAVSDGAAAGRVLRIHTGARDYIYRVRLDD
                     AEVEREVRRFRAAVRLSRRQLRALRRRAERALGDEHAYIFDAHLLMLEDRKLSEDVEA
                     VIRGERVNGEWAVKVVTDRLLAVYDEIKDDYLRERSSDIEDVTRRLLVALSGESLRGR
                     RLTEDAVVVAEELMPSTIAELDFTHIKAIATDMGGWTSHTAIIARGLGIPAIVGLRDF
                     YRHARTGDTVVVDTRAGEVVLHPSRERFDAYKAKEQARDGGAARAVAEEPRGPLLTED
                     GVEIVLRANVELPAEYEGVARFGARGIGLYRSEFLLTQRGALPDEDEQCSAYEEVGRV
                     AGADGAAVRLFDLGGDKLGGLALEADGERNPALGLRAIRFCLLREDVLRTQVRAVLRA
                     AARRPLDLVLPMISDLTEVRRARRVIEEERARLESEGREAGRIRVGAMIEVPSAVMVA
                     EKLAREVDFFSLGTNDLVQYMLAVDRGNDEVAPWFRSLHPAVLQAVSRTIEAARAASI
                     PAIVCGEMAATPAYAVVLVGLGAHELSMAASSIPRVRRVLSRVDSRVAAEVARECLDC
                     ATADEVEELVRVRFGERWPNLFPPETLPAPRTNV"
BASE COUNT          850 a         1820 c         1928 g          770 t
ORIGIN      
        1 cccttgaata aaagaagcat gacagccgtg ctgttcgccg tcctcctcgc cgtcgcgccc
       61 gcgtacagcg ccgtgacgaa gtacgaaatc gacaccgcgc actcgaacgt cgggttctcg
      121 atcccgatcc tcggcgggct gtcgagcgtg cgcggcaagt tcacggactt caaggtcgag
      181 atcacgtacg acgacaagga cgtgacgaag tcctcggtca acgccgtcat caaggccgcg
      241 agcatcgaca cgggcatcga gcggcgcgac gcgcacctca agaacgccga cttcttcgac
      301 gtggagaagt acccggagat caccttccag tcgaagcacg tcgagaagaa gggcaaggac
      361 ttcgtcgcgc acggcacgtt cacgatgcac ggcgtctcga aggagatcgc gctgcccttc
      421 acgctcaacg gcgtgcagcc cgccaaggac ggcaagaccg tgctcggcgt gacggcgcgc
      481 acgaccctca accgcaagga cttcggcgtc agcttcgcgc ggcccgacaa cccgaacttc
      541 ctcggcgaca acgtcgagat cgagctgagc ctcgtgacgc gcgccgccgc gcccgcgggc
      601 accgccgccg ccccggcccc ttcgggcaag tagtcgcacc gccgtcaacc cgtccgcgtc
      661 gtgaaacggt gacaggcgat tcttatcaaa gtccgtctat cacggtgagc gacttcgtcg
      721 cgcacgcgcc gccgcatctc ggcctgagtt tcctggccgg ggagcgcggc gcgtcgtcgc
      781 gtctgctcga cgtgccgcgc atccagaagt tgggccttgc gctcgcgggc ttcgcgcact
      841 acgtccacgc ggggcgcgtg caggtcgtcg gccagagcga gatcgccttc ctcacgcagc
      901 tcacggcaga gcggcggcaa gaggccatcc gcaacgtcga gttcgggaag atatcgtgcg
      961 tgctcgtgac gaagggcctc gtgccgcccg cggaattcgt cgccgcgtgc gaagaggctt
     1021 cgctcccgct cgtccagacg ccgctcgtca gctcggtcgc catcaacgcc gtcacggagt
     1081 atctggtcga agcgctcgcg ccgcgcgaga cgcggcacgg cgtgctgctc gacctctacg
     1141 gcctcggcgt gctgctcgaa ggcaaaagcg gcgtcggcaa gtcggagtgc gcgctcgacc
     1201 tcgtcgtgcg cggccaccgg ctcgtctcgg acgacgtgat cgaggtgaag cgcacggggc
     1261 cggagcagct cgtggggagc gcgcccgagc ttttgcgcga gctgatggag attcgcggcc
     1321 tcggcatcct gaacgtgcgc gacctgttcg gcgtctcctc gatcagcggg ccgaagcaga
     1381 tcgggctgac catccggctc gtgcgctggg aggagaccga ggaggttgac cggctcggcc
     1441 tcgaagcgcg cacgacggaa atcctcggcg tcgcggtgcc ttacttcctg ctgccggtca
     1501 gtccgggcag gaacctctcg acgctggtcg agacggcggt gcgcgtgcac ctgctgcgcg
     1561 tgcgcggcta caacgcggcg ggcgacttcg ccgcgcgcca cgcggagttg atcgagggcg
     1621 acttcaggcg cggcgagggc gcggcgtctt cgtcgtcgtc gccgggggag gacgggacgg
     1681 gggatgtcat ctaagaagag cggggcggag cgcggccagg ggtcggcgca cgcgcccgac
     1741 atcgtcatca tcaccggcct cagcggctcg ggcatgtctt cggcgaccaa cgccttcgaa
     1801 gacctcggct acttctgcgt ggacaacctg ccgctcactc tgctcccgac cttcgcgcgc
     1861 ctcgtccgcc ccgaagaccg ccacgcgcag cacatcgagc gcgccgcact cgtcatcgac
     1921 atccgcgagg gtcgcttcct ccgcgagttc cccgcgcggc tgaaagagct gcgcgcgtcg
     1981 ggcaaggatg tgcacgtcgt cttcctcgaa gcctcggacg acgcgctcgt ccgccgcgtc
     2041 agcgagacgc ggcggcccca cccggcggac gacggcggcg gcctcggcga agccatccgc
     2101 ggcgagcgcg ccgcgatgga ggacgtgcgc gcgctcgccg atcaggtcat cgacacctcg
     2161 gagcacaccg tccacacctt gcgccgcctc ctgctcgaac gcttcagccc ggacgcggac
     2221 ggcgcggaga tgcgcgtgca ggtcatcagc ttcggctaca agcacggctt cccgcgcgac
     2281 atcgatctga tgttcgacgt gcgccacctg ccgaacccgc acttcgtgcc ggagctgcgc
     2341 gacttctcgg gccacgaccc gcgcgtcgtc aagttcctga ccgcgagcga cgaggtcaaa
     2401 gagaccatcc gccgcttcgc cgagctggcc gactacctgc tgccgcttta tcaacgcgag
     2461 ggcaagtctt atctgaccat cggcatcggc tgcacgggcg gtcgccaccg ctccgtcatg
     2521 gtcgccaacg cgctcgcgcg ccacctccgc cgcggcggct tcgacgcgac cgccgtccac
     2581 agggacgtga gcaagaccaa aaggcagagg ggcgcggcgg ggaagtcgaa ggctaaaggc
     2641 gggacgcgtg aggcgggcaa agtgcgatag actacgcggc ggtcttcttt atcgaatcgg
     2701 cgcggcctcg gagttgtgaa tgtcaaaaga cgtgaacgaa gagagtgaga aaggcgcggc
     2761 ggcgcggcgc gtgggcggcg tggtggtgac gcacgggcag gtggcgaccg agctgctggc
     2821 cgccgccgag accatcatcg gcgcgatcaa gcacctgacc gccgtctcca tcgggtggca
     2881 cgacgacgtg gacgcggcga gcgccgaggt cgagcgcgcc atcgagcgcg tctccgaagg
     2941 gcacggcgtc ctgatcctga ccgacatgtt cggcgggacg ccgaccaaca tcgcgtcgat
     3001 gttcctgaag aagggcgagg tcgaggtggt gacgggcgtc aacctgccga tggtcatcaa
     3061 gctggcgggg caggccggcg gcgagagtct ggacgaggtg gcgcagcgcg tgcgcgacca
     3121 ggggcgcgag ggcatctacc tcgcgggcga gctgctggcg ggcgggggga agaaggcttg
     3181 atggtcgagc ggagcgtccg cgtggtcaac cgtctgggcc tgcacgcgcg cgcggcggcg
     3241 cggctggtgc gcacggcctc ggcgttccgg agcgcggtgc ggctggagcg cgccgatcgc
     3301 agcgcgaccg ccgaggcgaa gtcgatcttg agcgtgctga tgctcgcggc ctcgcgcggg
     3361 acggagctgc gcgcggtggc cgaggggccg gacgagaggg aagccctcga cgccgtgtgc
     3421 gagctgatcg agggcgggtt cggggaacag ccggagtgaa gcacccgaag ggcaagggcg
     3481 aggcgcgctg gcgcggcacc gccgtctcgg acggcgcggc ggcgggccgc gtgctgcgca
     3541 tccacacggg cgcgcgcgat tacatctatc gcgtcaggct cgacgacgcc gaagtcgagc
     3601 gcgaggtgcg ccgcttccgc gccgccgtgc gtttgtcgcg gcgccagctc cgcgctttga
     3661 gaagacgcgc cgagcgcgcg ctcggcgacg agcacgcgta catcttcgac gcgcacctgc
     3721 tgatgctcga agaccgcaag ctctcggagg acgtggaggc ggtcatccgc ggcgagcgcg
     3781 tcaacggcga atgggccgtg aaggtcgtca ccgaccggct gctcgccgtc tacgacgaga
     3841 tcaaggacga ctacctgcgc gagcgcagct ccgacatcga ggacgtgacg cggcgcctgc
     3901 tggtcgcgct gagcggcgaa tctttgcgcg gtcgtcggtt gaccgaggac gcggtcgtcg
     3961 tcgccgaaga gctgatgccc tcgaccatcg cggaactcga cttcacgcac atcaaggcga
     4021 ttgcgaccga catgggcggc tggacttcgc acacggcgat catcgcgcgc ggtctcggca
     4081 tccctgccat cgtcggcctg cgcgacttct accgccacgc gcgcacgggc gacacggtgg
     4141 tcgtggacac gcgcgcgggc gaggtcgtgc tccacccttc gcgcgagagg ttcgacgcgt
     4201 acaaggcgaa ggagcaggcg cgcgacggcg gcgcggcgcg ggcggtggcg gaagaaccgc
     4261 gcgggccgct gctgaccgag gacggcgtcg agatcgtgct gcgcgcgaac gtcgagctgc
     4321 ccgccgagta cgaaggcgtc gcgcggttcg gcgcgcgcgg catcgggctg taccgctcgg
     4381 agttcctgct gacgcagcgg ggcgcgctgc cggacgagga cgagcagtgc tcggcctacg
     4441 aagaggtcgg gcgcgtggcg ggcgcggacg gcgcggcggt gcgcctgttc gacctcggcg
     4501 gcgacaagct cggcggcctc gcgctcgaag cggacggcga gcgcaacccc gcgctcggcc
     4561 tgcgcgcgat ccgcttctgc ctgctaaggg aggacgtgct acgcacgcag gtgcgcgccg
     4621 tgctgcgcgc cgccgcgcgc cgcccgctcg acctcgtgct gccgatgatc tccgacctca
     4681 cggaagtcag gcgcgcgcgg cgcgtcatcg aggaggagcg ggcgcggctc gaatcggagg
     4741 ggcgcgaggc ggggcgcatc cgcgtcggcg cgatgatcga agtgccttcg gcggtgatgg
     4801 tcgccgagaa gctcgcgcgc gaagtcgatt tcttcagcct cggcacgaac gacctcgtgc
     4861 agtacatgct cgccgtagac cgcggcaacg acgaggtggc cccgtggttc cgctcgctcc
     4921 acccggcggt gttgcaggcc gtcagccgca ccatcgaggc cgcgcgcgcc gcctcgatcc
     4981 cggccatcgt ctgcggcgag atggcggcga cgccggccta cgcggtcgtg ctcgtcggcc
     5041 tcggcgccca cgagctgagc atggccgcct cttcgattcc gcgcgtgcgc cgcgtgctct
     5101 cgcgcgtgga cagccgcgtc gcggcagagg tcgcgcgcga gtgcctcgac tgcgcgaccg
     5161 cggacgaagt ggaggagctg gtgcgcgtgc gcttcggcga gcgctggccg aaccttttcc
     5221 cgccggaaac cttgccggcg ccgagaacga acgtttagaa tcgaacacag gaggcgcact
     5281 caacccccgc gcccccgttc attagaacct cgtcacccgg agccttctcc aggaaattcc
     5341 aatgagtgtg agacggtcag caatattg
//
LOCUS       DBNGOH010000962         4091 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1611437, whole genome shotgun sequence.
ACCESSION   DBNGOH010000962 DBNGOH010000000
VERSION     DBNGOH010000962.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4091)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4091)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4091
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1611437"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..980)
                     /locus_tag="AC159P_18775"
     CDS             complement(<1..980)
                     /locus_tag="AC159P_18775"
                     /inference="COORDINATES: protein motif:HMM:NF014312.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HIR3697540.1"
                     /translation="MKKVEGLKPDIEMLVAAHCGQRRCRMSFPTDRGARGSASGALLT
                     FVACLTLLVCVAGSRKPVDARSANRPPAVRLAEAGVLVIAYMEESRVALVDGATYRTL
                     ATLETGKNPHEVRVSPDQRHAYVAAGKSITAIDLRSRKVKATFDLGSYSAHDIRISRD
                     GSRIWAACGGAQTILELDSATGKILEAFRTDQQGSWFVEITPDERKIYTPNLEGKSVS
                     VIERATGKVKVIRFEHPVYGIDITPDGKQVWVSGGDLAVIETATDEVIARVKTSEAET
                     GRIRLTSDGKQVVVALSKKLAVFDAKTRRLIGETELASSPKVLALSGDSRR"
     gene            1388..1894
                     /locus_tag="AC159P_18780"
     CDS             1388..1894
                     /locus_tag="AC159P_18780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697541.1"
                     /translation="MGRRMFDGLHGGGTRVSVLLKGAALSVLAIHFTLALVSGYRAYY
                     QVWGLELHLPGRVLRDGSTVRTSVLTSGRTFVDVRLEMLQDGHTELLAARQVPRNDFA
                     VYDPRPQRAALTAALTPEVLARFRAGPALVRATAIGRPQWLRVPPPEVREFAVEVGHE
                     PADAQGRR"
     gene            2109..2690
                     /locus_tag="AC159P_18785"
     CDS             2109..2690
                     /locus_tag="AC159P_18785"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF024969.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose-7-phosphate isomerase"
                     /protein_id="HIR3697542.1"
                     /translation="MRSGLIAEHFASHRTVVEESAEQLRGPAEDVARALVECLRAGKK
                     VIAFGNGGSAAQASHLVGELLGRFKMTRQPFAAVALTSDAGTVTCIANDFGYEALFER
                     QARALAQAGDVALGFTTSGRSENVRRGLGAAKERGAVTVAVTGVNGLVGGSADHVVAV
                     PSDVTAHIQEVHLMLLHIWCIAIDEELGQAIHA"
     gene            2832..4046
                     /locus_tag="AC159P_18790"
     CDS             2832..4046
                     /locus_tag="AC159P_18790"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HIR3697543.1"
                     /translation="MNRVSAAALCLLLFACGAVTLSAQSGRRKTVQSTERTSDSATST
                     PTADASGAGREADAQSGAQGETVEGDVVRVETTLVTVPVSVRDRDGRYAPDLRREDFH
                     IFEEGIEQRIAYFATVDQPFTVALVLDTSGSTDLRLDDMQEAAIAFVARLKPQDRVLV
                     IAFDDRFDVLAEPTSDRAALTKAIRRARSGGGTRLYDAVEFVINKHLKNIDGRKAVVL
                     FTDGVDTTSRRATYESTVRQAEELDALIYPVSYAALGAGLRGITPPTFPTPGGRGGVI
                     IGSPFPRTGGGGGGVSRAEYERGNAYLNELARKTGGRFYRGDSLSNIAQAFEWVAEEL
                     RRQYSLGYYPKSAAQEGQRRQIKVRVDRPNLVVLSRDSYIHSRKKADTKEAGAQQLTE
                     AEAQTKHLVGIR"
BASE COUNT          734 a         1277 c         1379 g          701 t
ORIGIN      
        1 cgacggctat cgcccgagag ggcgagtact ttcgggcttg aggcgagttc ggtttcgccg
       61 atcaggcggc gggtcttcgc gtcgaaaacc gcgagttttt tcgataaggc gacgacgacc
      121 tgtttaccgt cggaggtgag cctgattcga ccggtctcgg cctcggaggt tttgactctg
      181 gcgatcactt catcggtagc cgtttcgatc acggcgaggt cgccgccgct gacccacacc
      241 tgtttcccgt cgggggtaat gtcgatcccg tagacagggt gttcgaaccg aatgaccttt
      301 accttacccg tggcgcgctc gatgacactg acgcttttcc cttcgagatt cggggtgtag
      361 atttttcgct cgtcgggtgt gatttcgacg aaccaggagc cttgctggtc tgttcgaaat
      421 gcttcgagaa tcttccccgt agccgaatcc agttcgagga ttgtctgcgc gcccccacag
      481 gcggcccaga tgcggctgcc gtcgcggctg atccgaatgt cgtgcgccga ataagagcct
      541 agatcgaagg tggctttgac ttttcgactc cgaagatcga tagcggtgat cgactttccg
      601 gcagcaacgt aggcgtgtcg ttgatcgggt gaaacgcgca cctcgtgagg gttcttaccc
      661 gtctcaagcg tggccagagt tcgataagtg gcgccgtcaa ccagagcgac ccgggattcc
      721 tccatgtatg cgattacgag aacgcccgcc tcagccagcc gcacggcagg cggacggttt
      781 gccgaccgcg catccaccgg cttccgactt cccgccacac acacgagcag cgtcaggcaa
      841 gccacgaagg tgagcaacgc gccggatgcc gatccgcgcg ctcccctgtc ggttggaaaa
      901 ctcatcctgc atctcctttg gccgcagtgg gccgcgacga gcatttcgat gtcgggtttc
      961 aacccttcga ctttcttcac cgattggcga actgttcgac gaatgttccg ttaacgagga
     1021 gtcttgccgc gaagacccga gcatagaagc cgaattctac ggcgcaagca taaatgtccc
     1081 gaagcgtttg ttcgaggtca tgagtcgtgc gactcgcggc aagtggtgtg ggggccgatc
     1141 cgaatatcct cggggcttat cgcaagcggt ttttcagccg ccggcgcgca ggatatttcc
     1201 ccgaacgcgg gagtgtctga atcatagccg tcaatgttaa gctgcgtgga tgcgtcgatc
     1261 gtctacgcct ggaagcaggc gatgagcgcc aaggagcaag agcgttttcg gcgcgtcgcg
     1321 ggcgaccagt taaaagaagc cgggtagcga ggtgtgagcg gtgaagggaa ccggacgagg
     1381 tagtcacgtg gggcggagaa tgttcgacgg cctccacggc ggcggcacgc gggtgtccgt
     1441 cctcttgaag ggcgcggctc tgtcggtgct cgccattcac ttcacactcg ccctcgtctc
     1501 ggggtatcgg gcgtactatc aggtgtgggg tcttgaactc cacctgccgg gacgggtgct
     1561 ccgcgacggt tcgaccgtcc ggacttcggt gctcacctcc gggcggacgt tcgttgacgt
     1621 gagactcgaa atgcttcagg acgggcacac cgaattgctg gcggcgcggc aggtgccgag
     1681 gaacgacttc gcggtgtacg acccgcgccc gcagcgtgcc gcgctgacgg cggccctcac
     1741 gccggaggtg ctggcgaggt tccgcgccgg gccggcgctg gtgcgcgcga cggcgattgg
     1801 tcggccccaa tggctgcgcg tgccgccgcc cgaggtgcgc gagttcgcgg tggaagtcgg
     1861 gcacgaaccg gcggatgcgc aagggcgaag gtagtccgcc gaacgagtca tgcgctcgtc
     1921 ccgcgctcgt tgttgccttc gtcgagagca gtgctctcga cgcgactatg aagagcgtgt
     1981 cgcaacactg tcggggcggg gcttgttcct gcccgccgtg acaggtatgt tcgacgccga
     2041 atcgcttgaa cgtcgagggg cagggacaag ccccgccccg acagcaaaca tggaacggcg
     2101 gaggtaacat gaggtcaggt ctcatcgcgg agcatttcgc ttcgcaccgc acagtagtcg
     2161 aagagagcgc cgagcagttg cgcgggccgg cggaggatgt ggcgcgcgcg ctggtcgagt
     2221 gtctgagggc ggggaagaag gtcatcgcct tcggcaacgg cgggagcgcg gcgcaggcga
     2281 gccacctcgt cggcgagctg ctcgggcgct tcaagatgac gcggcagccg ttcgcggcgg
     2341 tcgcgctgac gagcgacgcg ggcacggtca cgtgcatcgc caacgacttc ggctacgaag
     2401 cgctcttcga gcggcaggcg cgcgcgctcg cgcaggccgg cgacgtggcc ctcggcttca
     2461 cgacgagcgg gcgctcggag aacgtcaggc gcggactcgg cgcggcgaaa gagcgcggcg
     2521 cggtgacggt cgccgtcacg ggcgtcaacg gccttgtcgg cggcagcgcc gaccacgtcg
     2581 tcgcggtgcc gagcgacgtg acggcccaca tacaggaggt gcatctgatg ctgctgcaca
     2641 tctggtgcat cgccatcgac gaggagctgg ggcaggcaat tcacgcatag cggcgaaaag
     2701 gggtcgcctg actccacggt ttacaaaact ttaccttccc ttgaccgact ctttacgcgc
     2761 ccgtttgtga gacggcgcgt ccaagagcag accgaccgga gaaggatcag gccgtggaca
     2821 aaacaggaac catgaatcgc gtttcagccg ccgcgctctg cctcttactg ttcgcgtgcg
     2881 gcgcggtcac tttgtccgca cagtcggggc gcaggaagac cgtccaaagc acggagagaa
     2941 cttcggactc cgcgacgagc acgccgacgg cggacgcgag cggggcgggc agagaggcgg
     3001 acgcgcagag cggcgcgcag ggcgagacgg tcgagggcga tgtcgtccgc gtcgagacca
     3061 cgctcgtcac cgtgcccgtc agcgtccgcg accgtgacgg cagatacgcg cccgatctca
     3121 gacgcgaaga cttccacatc ttcgaggagg gcatcgaaca acggattgcc tacttcgcca
     3181 ccgtcgatca gcccttcacg gtcgcgctcg tgctcgacac gagcggctcg accgatctca
     3241 ggctcgacga tatgcaggag gcggccatcg ccttcgtcgc gcggctcaaa ccgcaagacc
     3301 gcgtgctcgt catcgccttc gacgaccggt tcgacgtgct cgccgagccg acgagcgacc
     3361 gcgccgcgct cactaaagcc atccgccgcg cgcgctccgg cggcggcacg cgcctctacg
     3421 acgcggtcga gttcgtcatc aacaagcacc tcaaaaacat cgacgggcgc aaggccgtcg
     3481 tgctcttcac cgacggcgtt gacacgacga gccgcagggc cacgtacgag agcaccgtgc
     3541 ggcaggcgga agagctggac gcgctgatct accctgtctc gtacgccgct ttgggtgcgg
     3601 gactgcgcgg catcacgccg ccgacattcc ccacgcccgg cggtcgcggc ggcgttatca
     3661 tcggctcgcc cttcccgcgc acgggcggcg gcggcggcgg cgtgtcgcgc gccgaatacg
     3721 agcgcggcaa cgcgtacctc aacgagctgg cgcgcaagac gggcgggcgc ttctaccgcg
     3781 gcgactctct gtcgaacatc gcgcaggcgt tcgagtgggt ggccgaagag ctgcgccgcc
     3841 agtacagcct cggctactac ccgaagtcgg cggcgcagga agggcagcgc aggcagatca
     3901 aggtgcgcgt cgataggccc aacctcgtcg tcctctcgcg cgacagctac atccactcgc
     3961 ggaagaaggc ggacaccaaa gaggcgggcg cgcagcaatt gacggaggcc gaagcgcaga
     4021 ccaagcacct cgtcgggatt cgttagagca cattgccgtt gcgtgtaccg gaaaaccggt
     4081 ctcaacacag a
//
LOCUS       DBNGOH010000963         3279 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3995377, whole genome shotgun sequence.
ACCESSION   DBNGOH010000963 DBNGOH010000000
VERSION     DBNGOH010000963.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3279)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3279)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3279
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3995377"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1066
                     /locus_tag="AC159P_18795"
     CDS             <1..1066
                     /locus_tag="AC159P_18795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HIR3697544.1"
                     /translation="IGVPRVAILHTWQNTQNEGWFRIEFDRLRIPYSYISEHTVRDTS
                     DLRAKYDVIIFPPVGGTAQSIVNGIPLRGDPIPWKASPLTPNMGNSPDTTDDMRGGMG
                     LVGVANLQRFVREGGLFVAVQNTVRVPVEYGLTTGVTVEEARQLQARGSIFNAAFTDR
                     RSPIAYGYGETLPIYFNQAPLFQVSEGPGAGGGGGGGAAAPGAAGGQRPTGRGSTDDP
                     DVVQAMPQAAPAPQRTPARPGEERLTDEQRQQLGPFYVPPHERARVVLRFAREEKSLL
                     VSGMLAGGSELANRPAVVDVTMGRGHIVLFATNPMWRHQTQGEFFLLFNAVLNFDNLG
                     AGRQPEPERPRESGRDGQEQ"
     gene            complement(1265..2065)
                     /locus_tag="AC159P_18800"
     CDS             complement(1265..2065)
                     /locus_tag="AC159P_18800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697545.1"
                     /translation="MRITRMLETTMRTSVALLLAALTYTTAQAKAPAVKVVRLAPTAG
                     GVVVFEPQAPRTPGSSPTAILNLDITIKNWEAASVELKTIFYKFTYGNGVPSGGVPPA
                     VLYFDIKKALSGDEAPFIPAGKERRVMLPSAYGHRYPLPTAVTVELRFAGFTDPVVVN
                     TSLGEYKNQTPTGSYRFPGRAEDLAPGERWYEGGTHVFGGLHRPSSSQRHAYDLGVRR
                     WDAQAKAWTNKRLPKNKDEVVDGKQNDHFLVYGRPVHAVAGGKVVRRA"
     gene            2271..2855
                     /locus_tag="AC159P_18805"
     CDS             2271..2855
                     /locus_tag="AC159P_18805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type sigma factor"
                     /protein_id="HIR3697546.1"
                     /translation="MAQHSTEDVTQMLAAWSDGDEAALERLVPVIHAELRRLARRYLA
                     RERPGHTLQTSALVNEAYLRLIKWKGARFQDRAHFFGVSAQLMRHILVDFARARPREG
                     AKEVRRVELEEGLVVSRGRDRDLVALDEALVELAKLDGRKARVVELRFFGGLSVEETA
                     EVLKVSPVTVMREWAKAKAWLYRELSAGGGGHAG"
     gene            2845..>3279
                     /locus_tag="AC159P_18810"
     CDS             2845..>3279
                     /locus_tag="AC159P_18810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HIR3697547.1"
                     /translation="MRAERWKRVDELLDAAMGLAPAERAALLDEACTGDAELRREVES
                     LLEAHDRAGDFIEAGALEAAAQNLAAEEAAEEEERARASAATSVGRRFGHYRILSLLG
                     AGGMGEVYLARDTRLGRPVALKLLPARFTDDEERVRRFEREAR"
BASE COUNT          510 a         1110 c         1148 g          511 t
ORIGIN      
        1 catcggcgtg ccgcgcgtcg ccatcctgca cacgtggcag aacacgcaga acgagggctg
       61 gttccgcatc gagttcgacc gcctccgcat cccctacagc tacatctccg aacacaccgt
      121 gcgcgacacg tcggacttgc gcgcgaagta cgacgtgatc atctttccgc ccgtcggcgg
      181 cacggcgcaa tcgatcgtca acggcatccc gctgcgcggc gacccgatcc cctggaaggc
      241 ttcgccgctg acgccgaaca tgggcaactc acccgacacg accgacgaca tgcgcggcgg
      301 catgggcctc gtcggcgtcg ccaacctgca aaggttcgtg cgcgagggcg gtctcttcgt
      361 cgccgtccag aacaccgtgc gcgtgccggt cgaatacggt ctcacgaccg gcgtgacggt
      421 cgaggaggcg cggcagttac aggcgcgcgg ctcgatcttc aacgccgcct tcacagaccg
      481 ccgcagcccc atcgcctacg gctacggcga gacgctgccc atctacttca accaggcgcc
      541 gctgtttcag gtcagtgaag ggccgggcgc gggaggtggc ggcggcggcg gcgccgccgc
      601 gccgggcgca gccggaggcc agcgccccac ggggcgcggc tcaacggacg accccgatgt
      661 cgtgcaggcc atgccgcagg ccgcgcccgc gccgcaacgc acgcccgcgc ggcccggcga
      721 ggagcgactc acagacgaac agcgccagca actcggcccc ttctacgtgc ctccgcacga
      781 gcgcgcgcgc gtcgtgttgc gcttcgctcg cgaagagaag agcctgctcg tcagcgggat
      841 gctcgcgggc gggtcggagt tggcgaatcg ccccgccgtc gtcgatgtca cgatgggccg
      901 cggccacatc gtgctcttcg ccaccaaccc gatgtggcgt catcaaacgc agggcgagtt
      961 cttcctgctc ttcaacgccg tgctgaactt cgacaacctc ggcgcgggcc gtcagcccga
     1021 acccgaacgc ccgcgcgaga gcgggcgtga cgggcaagag cagtagcaag gatgaactgc
     1081 cccgaagtca tcgacgaacg cccgaaggtc ttggccgccg cctgacgcgc gtgacatcgg
     1141 aaaccgtcgc ggtcgagggc gacggaaaag ggcgcgtgag cgtggtcgct cacgcgccct
     1201 tgaacgttcg cgctttcgtt cggcggaact tcgggttcga gtagaggcgt gcgtgcgcga
     1261 ttcgttacgc gcgccgtacg accttgccgc ccgcgaccgc gtggacaggc ctcccgtaga
     1321 cgaggaagtg gtcgttctgc ttgccgtcca ccacctcgtc cttatttttg ggcagccgct
     1381 tgttcgtcca cgcttttgct tgcgcgtccc aacgcctgac gccgagatcg tacgcgtggc
     1441 gctgcgagct ggagggacgg tggaggccgc cgaagacgtg cgtgccgccc tcgtaccagc
     1501 gctcgccggg cgcgaggtct tcggcgcgac cggggaagcg gtagctgccc gtcggcgtct
     1561 ggttcttgta ttcgccgagg ctggtgttga cgaccaccgg gtctgtgaag cccgcgaagc
     1621 gcagctcgac cgtgacggcg gtcggcagcg ggtagcggtg gccgtacgcg ctcggcagca
     1681 tcaccctgcg ctctttgccc gcagggatga acggcgcctc gtcgcccgag agcgccttct
     1741 tgatgtcgaa gtagagcacc gcggggggca caccgcccga cggcacgccg ttgccgtagg
     1801 tgaacttgta gaagatggtc ttcagctcga ccgaggcggc ctcccagttc ttgatggtga
     1861 tgtcgaggtt gaggatggcc gtgggcgacg agccgggcgt gcgcggcgcc tgcggctcga
     1921 acacgaccac gccgcccgcg gtcggggcga ggcggacgac tttgacggcg ggcgctttcg
     1981 cctgcgcggt cgtgtaggtg agcgccgcga gcagcagcgc gactgacgtg cgcatcgttg
     2041 tttcgagcat tctcgtgatt ctcatttcaa ttctcctcgt aaagcggcgg ggaagacttt
     2101 tccaaccgcc tgcgacacct aatgcgcccg gcgcgcgcgg aacctatcac ccgccgaaaa
     2161 ctattttttc ggcacgtctc gaaagtgttg gagggaggcg cgcgaggtgt gataaatttc
     2221 ggccacggac ccgacgtaat gaccccgccg cgccgccgcc gttaattctc atggcacagc
     2281 actcgacgga ggatgtcacg cagatgctcg cggcctggag cgacggcgac gaggccgcgc
     2341 tcgaacgtct cgtccccgtc atccacgccg aactccggcg cctcgcgcgc cgctacctgg
     2401 cgcgcgagcg accgggccac accctccaga cctccgcgct tgtcaacgag gcttacctca
     2461 gactcatcaa gtggaagggc gcgcgcttcc aggatcgcgc gcacttcttc ggcgtctccg
     2521 cgcagttgat gcggcacatc ctcgtcgact tcgcgcgcgc gcgtccgcgc gagggcgcga
     2581 aggaagtcag gcgcgtcgaa ctcgaagagg gtttggtcgt ctcgcgcggg cgcgaccgag
     2641 acctcgtggc gctcgacgag gcgctggtcg agctggccaa actcgacggg cgcaaggcgc
     2701 gcgtcgtcga gctgcgcttc ttcggcggcc tctcggtcga ggagacggcg gaggtcttga
     2761 aggtctcgcc cgtcacggtc atgcgcgagt gggcgaaggc gaaggcgtgg ctctacaggg
     2821 aactgagcgc cgggggcggc ggccatgcgg gctgagcgct ggaagcgtgt ggacgagttg
     2881 ctcgacgcgg cgatgggact cgcgcccgcc gagcgcgcgg cgctgctcga tgaagcctgc
     2941 acgggagacg cggaattacg gcgcgaggtc gagtcgctgc tcgaagcgca tgaccgggcc
     3001 ggcgacttca tcgaggcggg cgcgctcgag gcggcggcgc agaacctcgc ggcggaggag
     3061 gcggcagagg aggaggagcg cgcgcgggcg tccgccgcga cttcggtcgg gcggcggttc
     3121 ggccactacc ggattctttc actcctcggc gcgggcggca tgggcgaggt ctacctcgcg
     3181 cgcgacacgc gtctgggccg ccccgtcgcg ctcaagctcc tgcccgcgcg cttcacggac
     3241 gacgaggagc gcgtccgccg cttcgaacgc gaggcgcgc
//
LOCUS       DBNGOH010000964         3366 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_878157, whole genome shotgun sequence.
ACCESSION   DBNGOH010000964 DBNGOH010000000
VERSION     DBNGOH010000964.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3366)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3366)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3366
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_878157"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            199..1362
                     /locus_tag="AC159P_18815"
     CDS             199..1362
                     /locus_tag="AC159P_18815"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020577590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HIR3697548.1"
                     /translation="MNEEVRFFDGFCVELSEADAEALSNDPRVAYVEEDGLVRASTTQ
                     TNPPWGLDRIDQRDLPLNAQYNYTPTGAGVHVYVIDTGIRKSHGQFGGRANGTAFTSI
                     NDGNGSNDRNGHGTHVAGTVGGSTYGVAKGVTLHAVRVLDCNGDGTDATVIAGVDWVT
                     ANRVTPAVANISLGGGASTALDSAVQSMINAGVATAVAAGNDYGLDACTQSPARLAAA
                     ITVGSTTSTDAKSDFSNIGTCLDLFAPGSSIQSAWYTSDTATNTIGGTSMATPHVAGT
                     AALYLQNNPAASPATVRDQIVNTATTNHLTGTYYFSAVSGTHKGCLVGPTSGADFDLY
                     LQKWNGSAWVIVARSESVTSSETIFYTGTSGYYRWQIYSYSGSGGYNFWRLSP"
     gene            1577..2635
                     /locus_tag="AC159P_18820"
     CDS             1577..2635
                     /locus_tag="AC159P_18820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697549.1"
                     /translation="MKKLKLSLYTLAVALCLPALAFGQGRIEKLPASAAARHDVSKPL
                     REITPLKAKTNRRDFEPKRPAEPGGQFDDPVVQSSYSPLIAVTAGLNFEGVGEGLPGY
                     NVNVAPPDTTGDVGATQYVQWVNLSFAVFDKTSGLMVYGPAAGNTLWSGFGGPCETSN
                     DGDPIVQYDQLANRWMLTQFAVPGGAQGFWQCVAVSTTSDATGSYNRYAFQYNQFPDY
                     PKVSVWPDAYYVTFNMFSSFFTGARVCAWDRARMLAGQPATQQCFQLSTSFGSLLPAD
                     LDGPVQPPAGAPNYLLNFGTNILRLWKFHVDWVTPANTTLTGPTNIGAPPSRAAATAA
                     RASRSPAPTRSSTRSATA"
     gene            2635..3144
                     /locus_tag="AC159P_18825"
     CDS             2635..3144
                     /locus_tag="AC159P_18825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697550.1"
                     /translation="MYRLAYRNFGTHESLVVNHTVRTGGNNKKNQRTGIRWYELRNPG
                     GTPTVFQQGTYAPDADNYRWMGSAAQDKVGNLAVGFSVSSGSLRPSIRFAARGPADPV
                     NTLGSETNVFTGTGSQTQGLARWGDYATISVDPVDDCTMWFTTQYIATNGTFNWHTRI
                     NSFKLQGCE"
BASE COUNT          611 a         1262 c          993 g          500 t
ORIGIN      
        1 gatccgcctc cacgatccag accgggcctc ctgaagctac ctcgattctc tttcgccgga
       61 gaggcgacga atgttcccac atcccacagc ctatctcacc tttcacgacg gacggacggc
      121 caagccgcgc ggcacgaccg gcccgcgcgc ggcgcgacgc ccgccacctt ccaccagact
      181 gcccaaccct ccggaggtat gaatgaagaa gttcgttttt tcgacggctt ctgcgtcgag
      241 ctttcggaag cggacgccga ggccctgagc aacgacccgc gcgtcgccta cgtcgaggag
      301 gacggcctcg tccgcgcctc gaccacgcag accaacccgc cgtggggcct cgaccgcatc
      361 gaccagcgcg acctgccgct gaacgcgcag tacaactaca cgccgacggg cgcgggcgtc
      421 cacgtctacg tcatcgacac cggcatccgc aagtcgcacg ggcagttcgg cgggcgggcc
      481 aacggcacgg ccttcacctc gatcaacgac ggcaacggct cgaacgaccg caacggccac
      541 ggcacgcacg tcgcgggcac cgtcggcggc tcgacctacg gcgtcgccaa gggcgtcacg
      601 cttcacgccg tgcgcgtgct cgactgcaac ggcgacggca cggacgcgac cgtcatcgcg
      661 ggcgtcgatt gggtgacggc caaccgcgtc actccggcgg tcgccaacat cagcctcggc
      721 ggcggcgctt cgacggcgct cgactccgcc gtccagagca tgattaacgc gggcgtggcg
      781 accgcggtgg ccgccggcaa cgactacggc ctcgatgcct gcacgcagtc gcccgcgcgc
      841 ctcgccgccg ccatcaccgt cggctcgacg acctcgaccg acgccaagtc cgacttctcg
      901 aacatcggca cctgcctcga tctgttcgcg cccggctcct cgatccagtc cgcgtggtac
      961 acctccgaca cggcgaccaa caccatcggc ggcacctcga tggcgacgcc gcacgtcgcg
     1021 ggcacggcgg cgctctatct tcagaacaac cccgcggcct cgcccgccac cgtcagagac
     1081 cagatcgtca acaccgcgac gacgaaccac ctgaccggca cctactactt cagcgcggtt
     1141 tccggcacgc acaagggctg tctcgtgggg ccgacctcgg gcgcggactt cgacctctac
     1201 ttgcagaagt ggaacggctc ggcgtgggtc atcgtcgccc gctccgagag cgtcacgtcg
     1261 agcgagacca tcttctacac gggcacgtcc ggctattacc gctggcagat ttattcctac
     1321 agcggctcgg gcggctacaa tttctggcgg ctcagcccgt agtcacgacc gaccgccgcg
     1381 acccggagcc ggccgctcaa tcgacacggg agcggccggc ggcgaacaac cttgcggacg
     1441 aaccgggccg cgctctcatg gaaccgttca tgagcgaccg ttgcgatctc ctgacgactt
     1501 tcgttacgcc ttccgccgag gccgcgcggc gtggcgaacc tcgcccactt tcaacctcaa
     1561 tccgaccgga ggaaaaatga agaagctgaa actctccctc tacacactcg ccgtcgcgct
     1621 ctgcctgccg gcgctcgctt tcgggcaagg ccgcatcgag aagctcccgg cgagcgccgc
     1681 cgcccggcac gacgtgtcga agccgctgcg cgagatcacg ccgctcaagg cgaagacgaa
     1741 ccgcagagac ttcgagccga agcgaccggc ggagccgggc gggcagttcg acgaccccgt
     1801 cgtccaatct tcctactcgc cgctcatcgc cgtcaccgcg ggcctcaact tcgagggcgt
     1861 cggcgaaggg ctgcccggct acaacgtgaa cgtcgcgccg cccgacacga ccggcgatgt
     1921 cggcgccacg cagtacgtcc agtgggtcaa cctctccttc gccgtcttcg acaaaacgtc
     1981 gggcctgatg gtctacggcc ccgcggcggg caacactttg tggagcggct tcggcggccc
     2041 gtgcgagacc tcgaacgacg gcgaccccat cgtccagtac gaccagctcg ccaaccgctg
     2101 gatgctgacg cagttcgccg tgcccggcgg cgcgcagggc ttctggcagt gcgtcgccgt
     2161 ctcgaccacg agcgacgcca ccggcagcta caaccgctac gccttccagt acaaccagtt
     2221 ccccgactac ccgaaggtga gcgtctggcc cgacgcctac tacgtcacct tcaacatgtt
     2281 cagcagcttc ttcacgggcg cgcgcgtctg cgcgtgggat cgcgcgcgga tgctcgccgg
     2341 tcagcccgcc acgcagcagt gcttccagct cagcacctcc ttcgggagcc tcctgcccgc
     2401 agacctcgac ggccccgtgc agccgcccgc cggggcgccc aactacctgc tcaacttcgg
     2461 caccaatatc ctgcgcctct ggaagttcca cgttgactgg gtgacgcccg ccaacacgac
     2521 gctgacgggg ccgaccaaca tcggcgcgcc gccttcacgc gcggctgcga cggcggcgcg
     2581 tgcatcgcgc agcccggcac caaccagaag ctcgactcgc tcggcgaccg cctgatgtac
     2641 cgcctcgcct accgcaactt cggcacgcac gaatcgctcg tcgtcaacca cacggtcagg
     2701 acgggcggca acaacaagaa gaaccagcgc accggcatcc gctggtacga gctacgcaac
     2761 cccggcggca cgccgaccgt cttccagcag ggcacctacg cgcccgacgc ggacaactat
     2821 cgctggatgg gcagcgccgc gcaggacaag gtcggcaacc tcgccgtcgg cttcagcgtc
     2881 tcttcgggca gcctgagacc gagcatccgc ttcgccgcgc gcgggcctgc cgaccctgtg
     2941 aacacgctcg gctccgagac caacgtcttc accggcaccg gctcgcagac gcagggcctc
     3001 gcgcgctggg gcgactacgc caccatcagc gtcgatcccg tggacgactg caccatgtgg
     3061 ttcaccaccc agtacatcgc caccaacggc accttcaact ggcacacccg catcaactcc
     3121 ttcaagctgc aaggctgcga gtgaaaaagt aggcagtagg cagcaggcag taggcagtta
     3181 aaacagaagg gcgaagaggc ttgggaggta aagtgccaag cctcttccct cttcaagcaa
     3241 gaaagcaaga ggcttgagac gttagctctc aagcctcttg ctgttaccgc ctaccgccta
     3301 ccatgtcagc ggaagcgctc gatgtgttct ttggcgtact cctcgacggt gcggcgaatc
     3361 tctttg
//
LOCUS       DBNGOH010000965         2353 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1978707, whole genome shotgun sequence.
ACCESSION   DBNGOH010000965 DBNGOH010000000
VERSION     DBNGOH010000965.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2353)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2353)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2353
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1978707"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            202..996
                     /locus_tag="AC159P_18830"
     CDS             202..996
                     /locus_tag="AC159P_18830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697551.1"
                     /translation="MNTVLLSEKFGRMGARVRVGAVGFSHRGPGLDIGSDERGEFFDI
                     RLNPAERVEYEVVDLRPDLRHLLLLARREGAKEKYLCGHDERHWFVCAVPGQSVSTVT
                     NAMEALQPVEVRRAVAREVRRAKNRLRRRNEAFVRQGEWFFMPVPAWFRVDGRMVFRN
                     EPISRGAGSKPHMCQYLVRTGGETVMVSRHRPNGVTLAEYSNLLARNDKASKWDWAPM
                     RRNVNVFVRGRVRHPDHKTVVLDDWHHVAMNTEGKAPWAPAVIFLD"
     gene            1129..1686
                     /locus_tag="AC159P_18835"
     CDS             1129..1686
                     /locus_tag="AC159P_18835"
                     /inference="COORDINATES: protein motif:HMM:NF025637.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HIR3697552.1"
                     /translation="MALNGRVLLLNFSYEPLGTVGVARAMCLVLRGAVFVEEFEAGRF
                     LRSPSAAFAVPSVVRLRAYVNVRRRRQTAGMRRTRILIRDKFRCQYCGEKKLAHELTL
                     DHVTPRAQSGHSSPENLVAACVACNNRKGSRTPEQARMPLLTSQTNLRVGLDRVLLCH
                     YAESRPEWAKYLFLEDEEGARRARA"
     gene            1815..1892
                     /locus_tag="AC159P_18840"
     tRNA            1815..1892
                     /locus_tag="AC159P_18840"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1850..1852,aa:Val,seq:tac)
     gene            1995..2068
                     /locus_tag="AC159P_18845"
     tRNA            1995..2068
                     /locus_tag="AC159P_18845"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2029..2031,aa:Thr,seq:cgt)
     gene            2248..2321
                     /locus_tag="AC159P_18850"
     tRNA            2248..2321
                     /locus_tag="AC159P_18850"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2282..2284,aa:Ile,seq:cat)
BASE COUNT          411 a          780 c          790 g          372 t
ORIGIN      
        1 ggtgccgcat gtcggtcaag gccagacgta agcgcgtcgc acgaaggtgc cggcttgcac
       61 gcaggcgcag ccggcgcggc gcgggctagg gggcttcact gtgaatctcg cattcctttg
      121 tgtcggccct tgaggcgccc gcgccttggt cacaggcaac gagactccgc gaggggtctc
      181 cgtggaagca aaggaaagag tatgaacacc gtgttgttaa gcgaaaaatt cgggcgcatg
      241 ggggcgcgcg tcagggtcgg cgcggtgggc ttcagccacc gcggccccgg cctcgacatc
      301 ggctcggacg agcgcggcga gttcttcgac atccgcctca accccgcgga gcgggtcgag
      361 tacgaagtcg tcgatctgcg ccctgacctg cgtcacctgc tgctcctcgc gcgccgcgaa
      421 ggggcgaagg agaagtacct ctgcggccac gacgagcgcc actggttcgt ctgcgcggtg
      481 ccgggccaat cggtctcgac cgtgacgaac gcgatggaag cgctccagcc ggtcgaggtg
      541 cggcgcgccg tcgcgcgcga agtgcggcgc gcgaagaacc gtctccgccg acgcaacgaa
      601 gccttcgttc gtcagggcga gtggttcttc atgcccgtgc ccgcgtggtt tcgcgtggac
      661 ggccgcatgg tcttccgcaa cgagccgatc agccgcggcg ccgggagcaa gccgcacatg
      721 tgccagtacc tcgtccgcac gggcggcgag accgtgatgg tctcgcgcca ccgcccgaac
      781 ggcgtgacgc tggccgagta cagcaacctg ctcgcaagga acgacaaggc gagcaagtgg
      841 gactgggctc cgatgcgccg caacgtcaat gtcttcgtcc gcggtcgcgt gcggcacccg
      901 gaccacaaaa cggtggtgct cgacgactgg caccacgtcg cgatgaacac cgagggcaag
      961 gcgccgtggg cacccgcggt catcttcctc gactgaagat tgcgggcggc gccgcgcctg
     1021 cggcgaccct cacaccgccc gcaactcccc ttcccttttc ccgcgcccgg catcccgcgg
     1081 cgcgacatcg gtcatgattc gacatcgatc ttgaaaggag gtgccgtgat ggcattgaac
     1141 ggtcgtgtgc tgctgctcaa cttctcgtac gagccgctcg gcaccgtcgg cgtggcgcgc
     1201 gcgatgtgtc tggtgctgcg cggcgcggtc ttcgtcgaag agttcgaggc ggggcggttc
     1261 ctgcgctcgc cgtcggctgc gttcgccgtg ccgagcgtgg tgcggctgcg cgcttacgtc
     1321 aacgtccgcc gccgcaggca gacggcgggc atgaggcgca cgcgcatcct catccgcgac
     1381 aagttccgct gccagtactg cggcgagaag aagctcgcgc acgagctgac gctcgaccac
     1441 gtcacgccgc gcgcgcagag cggccactcg tcgccggaga atctggtggc ggcgtgcgtc
     1501 gcgtgcaaca accgcaaggg cagccgcacg cccgagcagg cgcgcatgcc gctgctcact
     1561 tcgcagacga acctgcgcgt cggcctcgac cgcgtgctgc tctgccacta cgcggagtcg
     1621 aggccggagt gggccaagta cctcttcctc gaagacgagg agggcgcgcg gcgcgcacgc
     1681 gcctgacgaa agggggagag gcgaacccgt ctctcccccg cgcacaccgg gtcggatagc
     1741 tgagacagat tagcgtcgct ctgaaaaagc gaagaggttg gcgcgattcc aactccgacc
     1801 gttgacacac cgcgggccgg ttagctcaga caggctagag cgccagtctt acaaactgga
     1861 agtcgaaggt tcgactcctt caccggccac cactttcaac ggggcgtggc tcaatcccgc
     1921 gcgcgcacgt gacggcgcgg ggtcgtgaga gcgcgaggac gactgcccct cacgccccgg
     1981 ccaacatttc atccggcgat gtagctccaa ccggaagagc agtcgtctcg taaacgacag
     2041 cgtgcgagtt cgaatctcgc catcgccttc acgcacgcga ggcccgcgca cccggcgtcg
     2101 gtcttctaaa ccgatccttc gaagagccgg gcggacaggc gcgaggttcg actcctccgc
     2161 gggccttcac acacacgatt aagtggtgtg gactgaggac tgagtgaaag acgagtgctt
     2221 ttccctcagt tctcccaaca cgcacgatgc ggaggagcac ggatagcttg tgcggcggtc
     2281 tcataaaccg acggtcgcgg gtgcaaatcc cgcctccgca attctcaagg actgagtgct
     2341 gaggactgag gac
//
LOCUS       DBNGOH010000966         1958 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_145848, whole genome shotgun sequence.
ACCESSION   DBNGOH010000966 DBNGOH010000000
VERSION     DBNGOH010000966.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1958)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1958)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1958
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_145848"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            243..1166
                     /locus_tag="AC159P_18855"
     CDS             243..1166
                     /locus_tag="AC159P_18855"
                     /inference="COORDINATES: protein motif:HMM:NF025910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3697553.1"
                     /translation="MMKMTDVFLLLLLLLIDPRAFPQTQQQTAAAAQTANALWQAQKW
                     EEAAKAYESLTRSEPNNGQAWFRLGSSLMSLQNFDAAVAPLARAAEILRGPQAYYALG
                     AAHAGTGNKVKSFESLRRAAETGFAQFERMKNDPHLAGLRDDAQFGGVLEAVERNARP
                     CKFSPEAKQLDFWVGRWDVQVSGQSVGTNVIERLEEGCLIMENWSGRGGSTGRSMNFF
                     NPVTNKWRQTYIGNNETIWEMSGEYKDGVMFYAGEMFPPRGAPVMVRVKFYNLSPDRV
                     RHTQDNSTDGGKTWTNVWDATYVRKKEAATQ"
     gene            1418..>1958
                     /locus_tag="AC159P_18860"
     CDS             1418..>1958
                     /locus_tag="AC159P_18860"
                     /inference="COORDINATES: protein motif:HMM:NF012698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HIR3697554.1"
                     /translation="MEGNFRIGEWLIAPRANRISTGGRVIHLEPKVMGVLTHLAGHAG
                     DVVTKESLLQSVWADTFVSEHVLKVAVSELRKALGDDARNPQYIETIPKSGYRLIAPV
                     SPTAADEEEAAAQPARNTRPGRKWWKLAAAALLLPALLLPAWQLTSLRRLPPPGRAPR
                     AINSVAVLPLKNLTGSSEGE"
BASE COUNT          389 a          634 c          615 g          320 t
ORIGIN      
        1 tggtgcaaac cggaatgccg acaggtaatc aggcacacgt ctcagttccc ccccaacccc
       61 gcgccttcgc cccacgcccg ccgactcacc ccgcagactc gattccatga aacgggcgcg
      121 ttgagaaaat ttgaaaaaaa ttaaccgaca attcaacgcc aagcagaacc ctttggactt
      181 aacatcaggg ccgatctcga cctcggtttc accgtataaa cccccgtccg taggagtgat
      241 gtatgatgaa gatgacagac gtgtttttat tgctcctcct gttgctgatc gacccgagag
      301 ccttcccgca aacacagcag cagacggcgg cggcggcgca gacggcgaac gcgctgtggc
      361 aggcgcagaa atgggaagag gcggcgaagg cgtacgagtc tctgacccgg agcgagccga
      421 acaacgggca ggcttggttc aggctcggct cctcgctgat gtctctgcaa aatttcgatg
      481 ctgccgtcgc gccgctcgca agggccgcgg aaatccttcg ggggccgcag gcttattacg
      541 cgctcggggc cgcccacgcc ggaacgggca acaaggtgaa atccttcgag agtttgcgtc
      601 gagccgcgga gaccggcttc gcccaattcg agcgcatgaa gaacgacccg catctcgccg
      661 gtctgcgcga cgacgcccaa ttcggcggcg tcttggaggc ggtcgagagg aacgcccgcc
      721 cctgtaagtt ctcgcccgag gcgaagcagt tggacttctg ggtgggccgc tgggacgtgc
      781 aggtcagcgg gcagagcgtc gggacgaacg tcatcgagcg gttggaggaa ggctgcctga
      841 tcatggagaa ctggtcgggg cggggcggct cgaccggcag gagcatgaac ttcttcaacc
      901 cggtcacgaa caagtggcgg cagacctaca tcggcaataa cgaaaccatc tgggagatga
      961 gcggggagta caaagacggc gtgatgttct acgcgggcga gatgttcccg ccccgtggcg
     1021 caccggtcat ggtgcgcgtc aagttttaca acctgtcgcc cgacagggtt cgccacacgc
     1081 aggacaactc caccgacggg ggcaagacct ggacgaacgt ctgggacgcg acctacgtca
     1141 ggaagaagga ggccgcgacg cagtgagggg cgcacgccgt ccgtaggctc cgtcccgcgc
     1201 ttcttgacga acgtgcacct ctcatcaagg gccgccgccc cggtcgccac gcctcgacgc
     1261 ggccaggccg gcggcctccg gtattttcac ggcaaagagg cgcctatact tgggaagctg
     1321 tttctgcgaa actgcgtctc gtcggcccgc acggtcgcgg tgtcattctc aagatatctc
     1381 attcaggttc catctccgcc cgtcacggag gccggctttg gaaggtaatt tccgcatcgg
     1441 cgaatggctg atcgccccgc gcgccaacag gatttcgacc ggcgggcgcg tcatccacct
     1501 tgagccgaaa gtcatgggcg tcctcacgca cctcgccggc cacgccggag atgtagtcac
     1561 gaaagagagc ctgctccagt ccgtatgggc tgacacgttc gtctcggagc acgtcttgaa
     1621 ggtggccgtc tcggagctac gcaaggcgct cggcgacgac gccaggaatc cgcaatacat
     1681 cgagaccatc cccaaaagcg gctaccgcct gatcgccccc gtttcgccga ccgcggcgga
     1741 cgaagaggag gccgccgcgc agccagcgcg gaacacccgc cccggacgaa agtggtggaa
     1801 actcgccgcg gctgccctgc tcctgccggc gctcctgttg ccggcatggc agctcacgtc
     1861 cctccgtcgc ctcccgccgc ccggtcgtgc gccgagggcg atcaactcgg tggcagtcct
     1921 cccgctcaaa aacctgaccg gcagctcgga gggcgagt
//
LOCUS       DBNGOH010000967         5763 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4177205, whole genome shotgun sequence.
ACCESSION   DBNGOH010000967 DBNGOH010000000
VERSION     DBNGOH010000967.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5763)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5763)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5763
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4177205"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            219..1649
                     /locus_tag="AC159P_18865"
     CDS             219..1649
                     /locus_tag="AC159P_18865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697555.1"
                     /translation="MLFIESRIADVFVSHAARVALLDGLQERGLVPVIRSSRLYCFER
                     ALMGAERVKELEQAVAAQSEAEGEVPYVRGDVFCFEGFAHFLVFGDAADEGQGLRAGI
                     VHDAETADPAQKLEDFCRNVKEALEGASRGAGHKADTFTFAGVEWKGQSGHAGTARIA
                     DGDGAGGDATKAPARAAESAERTRAVEIIEDAEARSFLRRLTEAEADGGAGVAGGAYE
                     PAREELIARLAGAGLARREVQVSCRKSGRSLFRLPSPDALAVVTASNAVCSDCGAAVA
                     DERAEELVTPTPLASKMLKDGAWLAGNLRATLAHAGMPAGEIAGRAGEADGEAQLLTA
                     VCGEPFLFVLSDGDFTAAHARRALEAEDQAGAAHLAVVATGKIHDEARARLRDHARRR
                     SRTGGEVELILAEGVEGAAAELRHAAERISRRALARELYDLDASAGFNVGHMLAARFR
                     LAHKTGALKDLAASAAGAHTGSPRET"
     gene            1828..3261
                     /locus_tag="AC159P_18870"
     CDS             1828..3261
                     /locus_tag="AC159P_18870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697556.1"
                     /translation="MRKALLIFPTVLLLAPTFAARAQEPSPSPATGYTRPRVVNQTAR
                     EEQQAQAQTKPTTGDAQKAPAAVPAATRPQQPPATAPTAQPSPAQTPTATAPASTTPT
                     MTPTTAPTSGPAIQPAVPYVPLQPARPLASNKARTKINEAQRLLRSRLTLTAATSPSL
                     EFVTVAALDQDDNEIHLVTLPKQTFLTRGTDVSLSTSKGMPVRLQVVRSNYVNTAVVV
                     SDAKGRQLTPLVVEYPIEKFGRFREMAYYTSAHPALVSPEVSRRGREYVRNMLDLAAK
                     RLKEKGKTIAPEIVDIAERLSIVEHVDHDRFRREDRAAIYNEVYALFALNELDTYRYS
                     VSSAGAGGMVQMIPSTYAMVRRMHPGVGLNPDFVAGMRNHGNALEAMLLYMQDTWNGL
                     ALNQEVLDAMRNNYATQPELASAGYNSNPARLPLYLRRGGAAWRTLIPRETQMYLQIY
                     KSFESLIPLKTRGDDDDAQKTATGGTQ"
     gene            complement(3381..3929)
                     /locus_tag="AC159P_18875"
     CDS             complement(3381..3929)
                     /locus_tag="AC159P_18875"
                     /inference="COORDINATES: protein motif:HMM:NF015438.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HIR3697557.1"
                     /translation="MNTDEIVGGMSHVAASPADGGRLELIIVRPAEGERVLPESVRVS
                     PEQGVEGDKWVRSRGHDTPDGGPDPRTQVTLMNARILRLISGEEGRMPLAGDNLIVDL
                     DLSDENIAPGQRLSVGEAVLEVTEVPHNGCGAFLDRYGRDAVKFVNSPEGKRLHLRGV
                     HAKVITAGTVRVGDEVRKVSSE"
     gene            complement(3963..4556)
                     /locus_tag="AC159P_18880"
     CDS             complement(3963..4556)
                     /locus_tag="AC159P_18880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019809184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HIR3697558.1"
                     /translation="MEIKDIEFFESPAEFRAWLEQHHATARELWVGYYRKGSGRQGMT
                     WPESVDEALCFGWIDGIRKRVDAESYANRFTPRRPRSVWSAVNIGRVGELTREGRMRP
                     AGVKAFEARDENRSRIYAYEQREQGLAEPYAKKMRRNKAAWKFFQAQTASYRKLVGSW
                     VSSAKKEETRLRRLEQLIEHSAAGRTIPQYTRAKKTR"
     gene            complement(4739..>5763)
                     /locus_tag="AC159P_18885"
     CDS             complement(4739..>5763)
                     /locus_tag="AC159P_18885"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3697559.1"
                     /translation="AQSEKFDVVKVAEGVYATLRKDSPGLAAEANTVFIINADDVVVV
                     DTNISASSTREVLAALRKLTGKPVRYVVNTHWHDDHVLGNQVYREAFPGVEFVAHART
                     REYLPTTGLSNRKQALAGVPGFLSFLKGQLEKNLSVSGDPLTEEERASYLNDIKIGER
                     FVAETPGAEVVLPSLTLEERLTLRRGERVIDILHLGPGHTGGDIVVHLPREGIVVAGD
                     LVVWPVPYVGAEQSHVGEWGATLDKLLALRPAVIVPGHGPLLRDDSYVRLIAAMFASV
                     KRQTEAAVARGETLEQSRKSVNLEEFRKQIAGESRFRGVLFRNYVAGPAVAAAYREAA
                     AKRRKQ"
BASE COUNT          946 a         2062 c         1845 g          910 t
ORIGIN      
        1 gacttgaaag gggccgcgca aaggttcaca ttgaactgtt gtgtgcgaag acggtacgta
       61 aatatcgcgg atacggcgat taaatgcgga taaatagctc gcattaattt caatctaata
      121 ccgccccgaa cggccaaatt cgattacgaa tcatgcccgt ggaaattatc ttcaagggcg
      181 tgataggatg cgcggccagg cggaggagtg cggtctttat gctattcatc gagagccgga
      241 tcgcggacgt tttcgtgagt cacgcggcgc gcgtcgcgct gctcgacggc ttgcaggaga
      301 ggggcctcgt gcccgtcatc cgctcgtcgc gcctctactg cttcgagcgg gcgctgatgg
      361 gcgcggagcg cgtgaaggag ttggagcagg ccgtggccgc gcagtcagag gccgaaggcg
      421 aggtgcccta tgtccgcggc gacgtgttct gcttcgaggg cttcgcgcac ttcctcgtct
      481 tcggcgacgc ggcggacgag gggcagggct tgcgcgcggg catcgtccac gacgcggaga
      541 ccgccgaccc cgcgcagaag ctcgaagact tctgccgcaa cgtgaaggag gcgcttgagg
      601 gcgcatcgcg cggcgcgggc cacaaggcgg acacgttcac cttcgcgggc gtcgagtgga
      661 aggggcagag tgggcacgca ggcactgcgc gcatcgcgga cggcgacggc gcgggcggcg
      721 acgcgacgaa ggcgcccgcg cgcgcagccg aaagcgcgga gcgcacgcgc gccgtcgaga
      781 tcatcgaaga cgccgaggcg agaagttttc tgcgtcgcct gaccgaggct gaggccgacg
      841 gaggcgccgg cgtcgcgggc ggcgcgtacg agcctgcgcg cgaagagttg attgcgcggc
      901 tggcgggcgc gggcctcgcg cggcgcgagg tgcaggtcag ttgccgcaag agcgggcgct
      961 cgctcttccg cctgccgtcg cccgacgcgc tggccgtcgt cacggcgagc aacgccgtgt
     1021 gcagcgactg cggcgcggcg gtcgccgacg agcgcgccga agagttggtg acgccgacgc
     1081 cgctcgcctc caagatgttg aaggacggcg cgtggctcgc gggcaatctg cgcgcgacgc
     1141 tcgcgcacgc gggaatgccc gcgggcgaga ttgccgggcg cgcgggcgag gccgacggcg
     1201 aagcgcagtt gctcacagcc gtctgcggcg agccgttcct cttcgtgctg agcgacggcg
     1261 acttcaccgc cgcgcacgcg cgccgcgcgc tcgaagccga agaccaggcc ggggccgcgc
     1321 acctcgccgt cgtcgccacc ggcaagattc atgacgaggc gcgcgcgcgc ctgcgcgacc
     1381 acgcccgccg acgctcgcgc acgggcggcg aggtcgagct gatcctcgcc gaaggcgtcg
     1441 agggcgccgc cgccgagctg cgccacgccg ccgagcgcat ctcgcgccgc gccctcgccc
     1501 gcgagcttta cgatctggat gccagcgccg gcttcaacgt cggccacatg ctcgccgcgc
     1561 gcttccgcct cgcgcacaag accggcgccc tcaaagacct cgccgcctcc gccgcgggcg
     1621 cgcacaccgg gagtccgcgc gagacatgaa gcagaggtca gaggctggag gtcagaggtc
     1681 agttaaaaca aactgccttc actgacctct ggcctccggc ctctgaccac tttttttggt
     1741 tgacaaccga aaccccgtcg ggcgaatctt aaagtcgcct cccgcgcgag aaggctatac
     1801 ccgccctgcg aagtacgaga acagacgatg agaaaagctt tattgatatt cccgaccgtg
     1861 ttgctccttg cgccgacgtt cgcggcccgc gcgcaggagc cgtccccatc gcccgcgacc
     1921 ggctacacgc ggccccgcgt cgtcaaccag acggcgagag aggagcagca ggcgcaggcg
     1981 cagacgaagc cgacgaccgg cgacgcgcag aaggctcctg ccgcggtgcc cgccgccacg
     2041 cgtccgcagc agccgccggc gacagcaccc acggcgcagc cttcgcccgc tcaaacaccg
     2101 acggcgacgg cgcccgcgtc cacgacgccg acaatgacgc cgacgaccgc gccgacgtcg
     2161 gggcccgcga ttcagcccgc cgtcccctac gtccctttgc agcccgcgcg cccgctcgct
     2221 tcgaacaagg cgcgcacgaa gatcaacgag gctcagcgcc tgctccgttc gcgcctgacg
     2281 ctgacggcgg ccacctcgcc ttcgctcgaa ttcgtgaccg tcgccgcgct cgatcaggat
     2341 gacaacgaga tacacctcgt gacgctgccg aagcagacgt tcctgacgcg gggcacggac
     2401 gtttcgctct cgacctcgaa gggcatgccc gtgcggctgc aagtcgtgcg ctcgaactac
     2461 gtcaacacgg cggtcgtcgt ctccgacgcg aagggccgac agctcacgcc gctcgtcgtc
     2521 gagtacccga tcgagaagtt cggtcgcttc cgcgagatgg cctactacac ctccgcgcac
     2581 cccgcgctgg tttcgcccga ggtctcgcgg cgcgggcgcg agtacgtgcg caacatgctc
     2641 gatctggcgg cgaagcgcct gaaggagaag ggcaagacca tcgcgcccga gatcgtggac
     2701 atcgccgagc ggctctccat cgtcgagcac gtcgatcacg accgcttccg ccgcgaggat
     2761 cgcgccgcga tctacaacga ggtctacgcg ctcttcgccc tgaacgagct ggatacctac
     2821 cgttactcgg tcagctcggc gggcgcgggc ggcatggtgc agatgatccc ctcgacctac
     2881 gcgatggtgc gccgcatgca tccgggcgtc ggcctcaacc cggactttgt cgccgggatg
     2941 cgcaaccacg gcaacgcgct cgaagcgatg ctgctctaca tgcaggacac ctggaacggc
     3001 ctcgcgctca accaggaggt gctcgacgcg atgcggaaca actacgccac gcagcccgag
     3061 ctggcctccg ccggctacaa ctcgaacccc gcgcgcctgc cgctctacct gcgccgcggc
     3121 ggcgccgcct ggcgcacgct catcccgcgc gagacgcaga tgtacctcca aatctacaag
     3181 agcttcgaga gcctgatccc gctcaagaca cgcggcgacg acgacgacgc gcagaagacc
     3241 gcgaccggcg gcacgcagtg agccgaagaa cctgacgccc ttacacgcga cctctcaaca
     3301 agcgccacgc cgaaaaacac agagggcacg ggggactaat cctccgtgcc ctcttcttca
     3361 ttcgtaatat cttgtcgcgc ttactcggac gacaccttgc gcacctcatc gccgacgcgc
     3421 accgtgcccg cggtgatgac cttggcgtgc acgccccgca ggtgcaggcg cttcccctcc
     3481 ggcgagttga cgaacttcac ggcgtccctg ccgtaccggt cgaggaacgc cccgcagccg
     3541 ttgtgcggca cctccgtcac ttcgagcacg gcctcgccga ccgagagccg ctggccgggc
     3601 gcgatgttct cgtcgctcaa gtcgagatcg acgatcaggt tgtcgccggc cagcggcatc
     3661 cgcccctctt cgcccgaaat caggcgcagg atgcgcgcgt tcatcagcgt cacctgcgtg
     3721 cgcgggtcgg ggccgccgtc cggcgtgtcg tgcccgcgcg agcgcaccca cttgtcgccc
     3781 tcgaccccct gctcggggct gacgcgcacg ctctccggca acacgcgctc gccctccgcg
     3841 gggcgcacga taatcaactc caacctgccg ccgtccgccg gcgacgccgc gacgtgggac
     3901 atgcccccca cgatctcgtc cgtattcatc atcaggatga ccctcgaaag ttcatgtgac
     3961 tgttagcgcg tcttcttcgc gcgcgtgtac tgtggtatcg tgcggcccgc ggcggagtgc
     4021 tctattagct gctccaaccg cctgagccgc gtctcttcct tcttcgcgct cgacacccac
     4081 gaaccgacca gcttgcggta cgaagccgtc tgcgcctgaa agaacttcca cgccgccttg
     4141 ttccgcctca tcttcttcgc gtacggctcg gccagcccct gctctctctg ctcatacgcg
     4201 tagatcctcg aacgattctc gtcccgcgcc tcgaacgcct tcacgcccgc gggacgcatc
     4261 cgtccctcgc gcgtcaactc cccgacgcgc ccgatgttca ccgcgctcca cacgctgcgc
     4321 gggcggcgcg gcgtgaagcg gttcgcgtag ctctccgcgt ccacgcgctt cctgatgccg
     4381 tcgatccacc cgaagcacag cgcctcgtcc acggactccg gccacgtcat gccctgcctg
     4441 cccgaaccct tcctgtaata accgacccac agctcgcgcg ccgtcgcgtg gtgctgctca
     4501 agccacgcgc ggaactccgc cggcgattcg aagaattcga tgtctttgat ttccaagctg
     4561 accaagttgt aatcactgac tacttgaccc gctgtcgctg tagccgcttg acccgcgcgc
     4621 cgcagtatag cattcgcccg attaacgcga acatagaccg cggccagcgg tttgatgatg
     4681 ccgtcaacgc gtgatacgtc tcactcaccc cgcaagtttg gccgcgcggt ggtcacggct
     4741 attgcttccg tctcttcgcc gccgcctccc tgtaggcggc ggccaccgcc ggccccgcga
     4801 cgtagttgcg gaacaagacg cccctgaatc gagactcgcc cgcgatctgt ttgcgaaact
     4861 cttcgaggtt cacgctcttg cgcgactgct ccagcgtctc gccgcgcgcc accgccgctt
     4921 cggtctgccg cttcacggag gcgaacatcg cggcgatgag cctgacgtac gagtcgtcgc
     4981 gcagcagcgg cccgtggccg gggacgatga cggcggggcg cagcgcgagg agcttgtcga
     5041 gcgtcgcacc ccactcgccg acgtgcgact gctccgcgcc gacgtagggg acgggccaga
     5101 ccaccagatc gcccgcgacg acgatgcctt ctcgcggcag gtgcaccacg atgtcgccgc
     5161 cggtgtgccc cggcccgagg tggaggatgt cgatgacgcg ttcgccgcgc cggagcgtca
     5221 gacgctcttc cagcgtgagc gaagggagca cgacctccgc gccgggagtc tccgcgacga
     5281 aacgctcgcc gatcttgatg tcgttcagat agctcgcccg ctcctcctcg gtcagagggt
     5341 cgccgctgac gctcaggttc ttttccaact gacctttgag gaacgagagg aagccgggga
     5401 cgcccgcgag cgcctgcttg cggttggaga ggccggtcgt cggcaggtac tcgcgcgtcc
     5461 gcgcgtgcgc gacgaactcg acgccgggga aggcttcgcg gtagacctga ttgccgagga
     5521 cgtggtcgtc gtgccagtgc gtgttgacga cgtacctcac cggcttgccc gtcagcttgc
     5581 ggagcgcggc cagcacctcc cgcgtcgagg acgcgctgat gttcgtgtcc acgacgacca
     5641 cgtcgtccgc gttgatgatg aagaccgtgt tcgcctcggc ggcgaggccc ggcgagtctt
     5701 tgcgcaacgt cgcgtagacg ccctccgcga ccttcaccac gtcgaacttc tcggactgcg
     5761 cgg
//
LOCUS       DBNGOH010000968         3260 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4544727, whole genome shotgun sequence.
ACCESSION   DBNGOH010000968 DBNGOH010000000
VERSION     DBNGOH010000968.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3260)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3260)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3260
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4544727"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..403)
                     /locus_tag="AC159P_18890"
     CDS             complement(<1..403)
                     /locus_tag="AC159P_18890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697560.1"
                     /translation="MTFTNNKILPVALVAALLGGSVGAFVMRPGKQAATTEVAQTTPV
                     LRSNFDATSSTTSADQTLPVSTNNDTVANNLDLSDGEMAAYRQGFNDGVKAAREGTLS
                     SQSLAAPATRTVTRTRYVRNGGSSSRRVYYDY"
     gene            931..2100
                     /locus_tag="AC159P_18895"
     CDS             931..2100
                     /locus_tag="AC159P_18895"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HIR3697561.1"
                     /translation="MSASKAKFRLASRLERVGFSDIVKVRNRIMELRAGGATVFQFEG
                     GEPYLNTPEPIKEAMTRALAENRTRYAPSSGIPELRAAIAAKVRERNRIDVREEDVLV
                     MTGGMQGLFGAFQSVVDPGDEVLVFAPFWAPIRDLISHCRGEAVLVPTAEARRAGLAE
                     TLARYATPRTRAVYYNTPQNPNGVVFTREEAEVVARFAQERDLVVVADEAYEDLVYEG
                     EHVSIASLEGMLERTITCFTLSKSYSMTGWRVGYAAAAEPWMTGLKKCLLYSTNGVST
                     PSQWAALAAFGLPADFHEGMRAAYRERRDLLIGGLNELGFSCDAPAGAFYAFPDASHI
                     NPDSRAAAETLLERARVATVPGIVFSEHGEGHLRFSFSTSLEVIRTGLDSMRRNL"
     gene            2137..3255
                     /gene="thiL"
                     /locus_tag="AC159P_18900"
     CDS             2137..3255
                     /gene="thiL"
                     /locus_tag="AC159P_18900"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HIR3697562.1"
                     /translation="MSDERSGDGNDFPSHGKSSPRRSTRRGEFDFINRIGRREQKRLD
                     AKNKHSALSTQHSALIKGIGDDAAVLGQRTGSETVITTDLLVEGIDFALDNFKTAPRD
                     IGHKALAVSLSDIAAMGARPRFALLSVGVPAACWDTPFLDHFYRGVFALADTHGVRLI
                     GGDISRTPERVVVASIVLGEVARGRAVLRSGARAGDLVYVTGALGGAAAGLRLLQRRA
                     HTGADEKLSRPQRQLVLRQTRPTPRVAWGRLLGERRLATAMIDVSDGLSSDLAHLCRE
                     SGVGAHVEAARLPLDPQLSRPAFSDEALPHALHGGEDFELLFTVRPRDAAKLPREVEG
                     VRATRVGFVTADAGRVRLDEGDRVVNLKPAGFEHFGRS"
BASE COUNT          546 a         1183 c          977 g          554 t
ORIGIN      
        1 cgtagtcgta gtagacgcgg cgcgaagagc tgccgccgtt gcggacatag cgcgtgcgcg
       61 tcacggtgcg ggtcgccggg gccgccaggg actgcgacga cagggtgcct tcgcgcgcgg
      121 ccttcacgcc gtcgttgaag ccctgtcggt aggccgccat ctcgccgtcg gaaaggtcga
      181 ggttgttggc gacggtgtcg ttgttcgtgc tcacggggag cgtctggtca gccgaggtcg
      241 tgctggaggt cgcgtcgaag ttgctcctga gcacgggcgt ggtctgtgcc acttcggtcg
      301 tcgcggcctg cttgccgggg cgcatcacga aggctccgac tgaaccgccc agcagggcgg
      361 cgacgagcgc gaccggaaga attttgttat tcgtgaaagt catgggtcgg tgtctcctga
      421 gattcttgat ccaaatttga tgacgcacca cgtacatggc cgcgccctac tcttttggca
      481 aataccgttc ctctattccg cacacccctt cgatagccgt tgagtttaac aagaacactt
      541 cacggcttca tgtttcccgg caaaaattcc gcgtttgtgc tcgaatcgta agaatgcaac
      601 cgtatcgcaa caatcacccc tttcccacac cgtatacgaa atggattgca cccgacccca
      661 tatcctcaac aatcagtagg cagtaggcag cgggcagtag gcggtaagca gttaaagcag
      721 gaggcttgag tgcgaacggc tcaagcctcc tcctctttct tcttgacgaa cgaagatgct
      781 tagcaattca tctctcaagc ctcttgtttt tgactgccta ccgcctactg cctactgcct
      841 actgtttccg cccgcttcct aattgttcgg gtcagcggct ataatccgcg cttgcacacc
      901 cgaaacacca ccggaggttc accgccacaa gtgtcagcgt caaaggccaa attccgcctc
      961 gcttcgcgcc tcgaacgggt cggcttctcc gacatcgtca aggttcgcaa ccgcatcatg
     1021 gaactgcgcg cgggcggcgc aaccgtcttc cagttcgagg gcggcgagcc gtacctcaac
     1081 acgcccgagc cgatcaagga ggccatgacg cgcgctctgg ccgagaaccg gacgcgctat
     1141 gccccttcgt ccggcatccc ggagctgcgc gccgccatcg ccgcgaaggt gcgcgagcgc
     1201 aaccgcatcg acgtgcgtga agaggacgtg ctcgtcatga ccggcggcat gcaggggttg
     1261 ttcggcgcct tccagtcggt ggtcgatccc ggcgacgagg tgctggtctt cgcgcccttc
     1321 tgggcgccga tcagagacct catctcgcac tgccgcggcg aggccgtgct cgtcccgacc
     1381 gcagaggcgc gccgcgccgg tctggccgag acgctcgcgc gctacgcgac cccgcggacg
     1441 cgcgccgtct actacaacac gccgcagaac ccgaacggcg tcgtcttcac gcgcgaagag
     1501 gccgaggtgg tcgcgcgctt cgcgcaggag cgcgacctcg tcgtcgtcgc cgacgaggcg
     1561 tacgaagacc tcgtctacga aggcgagcac gtctccatcg cttcgctcga agggatgtta
     1621 gagcgcacca tcacctgctt cacgctctcg aagtcctact cgatgacggg ctggcgcgtc
     1681 ggctacgccg cggccgccga gccgtggatg acgggcctca agaaatgcct gctctactcg
     1741 accaacggcg tctcgacgcc ctcgcagtgg gccgcgctcg ccgccttcgg cctgcccgcc
     1801 gacttccacg aagggatgcg cgccgcctac cgcgagcggc gcgacctcct gatcggcggt
     1861 ctcaacgaac tcggcttctc gtgcgacgcg cccgccggcg ccttctacgc cttccccgac
     1921 gcctcgcaca tcaaccccga cagccgcgcc gccgccgaga cgctcctcga acgcgcgcgg
     1981 gtcgccaccg tccccggcat cgtcttcagc gaacacggcg agggccacct ccgcttctcc
     2041 ttctcgacct ccctcgaagt catccggacg ggcctcgact cgatgcgccg caacctttaa
     2101 cgaagcgacg cggcgagtaa aagcgagtga tgagtgatga gtgatgagag gtcaggtgac
     2161 ggaaacgact tcccttcaca cggcaagtcc tcgcctcgac gctccacgcg acgcggcgag
     2221 ttcgacttca tcaaccgcat cggacggcga gaacagaaac gactcgacgc aaagaacaag
     2281 cactcagcac tcagcactca gcactcagca ctcattaaag gcatcggcga cgacgccgcc
     2341 gtcctcggcc aacgcacagg ctccgagacc gtcatcacca ccgaccttct cgtcgagggc
     2401 atcgacttcg ccctcgacaa cttcaagacc gcgccgcgcg acatcggcca caaggcgctc
     2461 gccgtctcgc tctccgacat cgccgcgatg ggcgcccgcc cgcgcttcgc gctcctctcc
     2521 gtcggcgtgc ccgccgcgtg ctgggacacg cccttcctcg accactttta ccggggcgtc
     2581 ttcgcgctcg ccgacacgca cggagtccgt ctcatcggcg gcgacatctc gcgcacgccc
     2641 gagcgcgtcg tcgtggcttc catcgtgctc ggcgaagtcg cgcgcgggcg cgcggtcttg
     2701 cgttcgggcg cgcgggcggg cgacctcgtc tacgtcaccg gggcgctcgg cggcgccgcc
     2761 gccggactcc gcctcctgca acggcgcgcg cacacgggcg cggacgagaa gctttcacgc
     2821 ccgcaacgtc aactcgtcct ccgccagacg cgccccacgc cgcgcgtcgc atggggccga
     2881 ctgctcggcg agcggcgact cgccaccgcg atgatcgatg tcagcgacgg cctctcctca
     2941 gacctcgcgc acctctgccg cgagagcggc gtcggcgcgc acgtcgaagc cgcgcgcctg
     3001 cccctcgacc cgcagctctc gcgccccgcg ttctccgacg aagccctccc gcacgcgctc
     3061 cacggcggcg aagacttcga actcctcttc accgtccgcc cgcgcgacgc cgcgaagctg
     3121 ccgcgcgaag tcgagggcgt ccgcgccacc cgcgtcggct tcgtcaccgc cgacgccggg
     3181 cgcgtccgac tcgacgaagg cgaccgtgtc gtgaatctca agcccgccgg gttcgagcac
     3241 ttcggacgct cttgagacgg
//
LOCUS       DBNGOH010000969         3677 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2713259, whole genome shotgun sequence.
ACCESSION   DBNGOH010000969 DBNGOH010000000
VERSION     DBNGOH010000969.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3677)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3677)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3677
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2713259"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..698
                     /locus_tag="AC159P_18905"
     CDS             <1..698
                     /locus_tag="AC159P_18905"
                     /inference="COORDINATES: protein motif:HMM:NF012936.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0070981 - L-asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HIR3697563.1"
                     /translation="GALPGGMPGRLTAPLRNAKKFARSAALDFEERYLGYGTYFTDEA
                     KYDIYSDRLREETRGLDAYRHHRRYFERCREAAPLNRLLYVDLKTFLPCLNLDYTDKT
                     AMATTLEVRVPFLNVEMVELAARMPPALKLRGLKRKYILKRAAERLLPFDVVWRKKAG
                     FGAPFRAWLRGPLRPLVEDLLSEETVRRRGLFRPSEVRRVVEQNYTGREDYSLQVLQL
                     LTLEMWHRIFIDA"
     gene            783..1955
                     /locus_tag="AC159P_18910"
     CDS             783..1955
                     /locus_tag="AC159P_18910"
                     /inference="COORDINATES: protein motif:HMM:NF016104.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000009 - alpha-1,6-mannosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0004376 - GPI mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006506 - GPI anchor biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyltransferase family protein"
                     /protein_id="HIR3697564.1"
                     /translation="MKRKHARAKQTATGEHATGGGTVAALRRLDWQTVALVLAAKALL
                     VVYGAAAFTVLQDQWPGGWLEIWNRWDAPHYLAIARQGYVSTGEESRWIVFYPLYPWL
                     VRVAAFVLRDYLLSAFVVSALASVAAALLLQRLAALDEEERAARAAVFFMLAFPTAYF
                     LHVGYTESLFMALALGSLLAARRRAWHVAGILGALAALTRVNGLLLIPALAAEAYAEY
                     RDEGRRQPRPEWAWIALVASGFAVYLALNYYALGDPFAFLSIQKEHWFRSFTWPWVSV
                     GDAWRSARWRTPSDAVMVGWMELLFALLGLGCTIWCAFRLRASYAVWMGANWLLWTST
                     KFLLSVPRYTLIMFPVYILFARAAVKRPALGGAIALWSLLLLALFAAQFARGHWAF"
     gene            2089..3138
                     /locus_tag="AC159P_18915"
     CDS             2089..3138
                     /locus_tag="AC159P_18915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020526443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1028 domain-containing protein"
                     /protein_id="HIR3697565.1"
                     /translation="MRIMLTLTLLAIVCAGLLLLFARARKTEAPPEAATLAHAAPRPL
                     RPVNTYSIVARDPATGQLGVAVQSHWFSVGSIVSWAESGVGAVATQSFVDPAYGKLGL
                     DLMRTGKTAPDALRSLLAGDEGRDVRQVAMIDAGGRVEAHTGAKCIDAAGHKVGRDYS
                     VQANMMANERVWPAMSQAFETARGDLAERMLAALEAAEAAGGDIRGKQSAALVVVSAK
                     PTGRPWADRVFDLRIDDHPQPLPELRRLVTLQRAYNHMNAGDLAVEKKDNEAALREYS
                     AAEKLVPDNLEMTYWHAIALVNMNRTDEALPMFRRVFQADPNWRTLTTRLPKSGILPD
                     DRKLLDRITAVGGTR"
     gene            complement(3257..>3677)
                     /locus_tag="AC159P_18920"
     CDS             complement(3257..>3677)
                     /locus_tag="AC159P_18920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697566.1"
                     /translation="VTLLLTGGVSIQSKTPVQRTGVITGALSYPSDFDFPRMIVCAEA
                     MDSRSIHCTDKRTTNRRSRKVTYRLSVPAGSYYVFATIVNGEAPTADYRGYKAYYSEF
                     VVCGLSVNCPSHAPVKVTVRAGRTSTGIDPGDWYVNN"
BASE COUNT          607 a         1308 c         1144 g          618 t
ORIGIN      
        1 cgggcgcgct ccccggcggg atgccgggcc gcctgaccgc gcccctgcgc aacgcgaaga
       61 agttcgcgcg cagcgccgcg ctcgacttcg aggagcgcta cctcggctac ggcacctact
      121 tcacggacga ggcgaagtac gacatctaca gcgaccgcct gcgcgaggag acacgcgggc
      181 tggacgccta ccggcaccac cgccgctact tcgagcgctg ccgcgaggcg gcgccgctca
      241 accgcctcct gtacgtcgat ctgaagacct tcctgccgtg cctcaacctc gactacacgg
      301 acaagacggc gatggcgacg acgctcgaag tgcgcgtgcc cttcctcaac gtcgagatgg
      361 tggagctggc ggcgcggatg ccgcccgcgc tgaaattacg cggcctgaag cgcaagtaca
      421 tcctcaagcg cgccgccgag cggctgctcc ccttcgacgt ggtgtggcgc aagaaggcgg
      481 gcttcggcgc ccctttccgc gcgtggctgc gcggccccct gcggcctctc gtcgaagacc
      541 tcctctcgga agagacggtc aggcgccgcg gcctcttccg cccttcggag gtgcgccgcg
      601 tcgtcgaaca gaactacacg ggccgcgaag actacagcct gcaagtcctg caactgctga
      661 cgctggagat gtggcaccgg attttcatcg acgcgtagac gcggcgcgcg ttttcctcgc
      721 cgctacgcgg cgaaacgaca ccgcgactgc tgaacattcc gcgacttccg aacgttccga
      781 cgatgaaacg caaacacgca cgcgccaaac agacggccac cggcgagcac gcgaccggcg
      841 gcggcacggt cgcggcgttg cggcgactcg actggcagac ggtcgcgctc gtgctcgccg
      901 cgaaggcttt gctcgtcgtc tacggcgcgg cggccttcac ggtgttgcag gatcagtggc
      961 cgggcgggtg gctcgaaatc tggaaccgtt gggacgcgcc gcactacctc gccatcgcgc
     1021 ggcagggcta cgtctcgacc ggcgaggaga gccgctggat cgtcttctac ccgctctacc
     1081 cgtggctcgt gcgcgtcgcc gccttcgtcc tgcgtgatta tctattgagc gccttcgtcg
     1141 tctcggcgct cgcttccgtc gccgccgcgc tcctgctgca aaggctcgcc gcgctcgacg
     1201 aagaggagag ggcggcgcgc gccgcggtct tcttcatgct cgccttcccg accgcttact
     1261 tcctgcacgt cggctacacg gagagcctgt tcatggcgct cgcgctcggc tcgctgctcg
     1321 cggcgcgtcg gcgcgcgtgg cacgtggccg gcatcctggg cgcgctggcc gcgctcacgc
     1381 gcgtcaacgg cctgctgctc atccccgcgc tcgcggcgga agcctacgcg gagtaccgcg
     1441 acgaaggccg acggcagccg cgccccgagt gggcatggat cgcgctcgtg gcctcgggct
     1501 tcgccgtcta cctcgcgctc aactactacg cgctcggcga cccgttcgcc ttcctcagca
     1561 tccagaagga gcactggttc cgttccttca cgtggccgtg ggtgagcgtc ggcgacgcgt
     1621 ggcgctcggc gcggtggcgc acgccctcgg acgccgtgat ggtcggctgg atggaactgc
     1681 tcttcgcgct gctcggcctc ggctgcacga tctggtgcgc cttccgcctg cgcgcctcct
     1741 acgccgtctg gatgggcgcc aactggctcc tctggacgag cacgaagttt ctcttgagcg
     1801 tgccgcgcta cacgctgata atgttccccg tctacatcct gttcgcgcgc gcggcagtaa
     1861 aacggcccgc actcggcggc gccatcgccc tctggtcgct gctcctcctc gcactcttcg
     1921 ccgcacagtt cgcccgcggc cactgggcgt tttgaaaaag cagtaggcag caggcagtag
     1981 gcagtcagtc gagcgaggac ggcgttgacg cttgcgccct gactgttcga tggaaattgt
     2041 ttttactgcc tgctgcctgc tgcctactgc ttttacggag tggtgaagat gagaatcatg
     2101 ctgacgctga cgctgctcgc catcgtgtgc gcgggcctgc tcctgttgtt cgcgcgcgcg
     2161 cgcaagaccg aagcgccgcc ggaggcggcc acgctcgccc acgcggcgcc gcgcccacta
     2221 cggcccgtca acacgtattc gattgtcgcg cgcgaccccg cgacggggca gctcggcgtc
     2281 gccgtccagt cgcattggtt ctcggtcggc tccatcgtct cctgggccga gtcgggcgtc
     2341 ggcgccgtcg ccacgcagtc cttcgtcgat cctgcttacg gcaagctcgg gctggatttg
     2401 atgcggacgg gcaagaccgc gcccgacgcc ttgaggtcgc tgctcgcggg cgacgaaggg
     2461 cgcgacgtgc ggcaggtcgc catgatcgac gccgggggcc gcgtcgaagc gcacaccggc
     2521 gccaagtgca tcgacgccgc cggccacaag gtcggcagag actactccgt ccaggccaac
     2581 atgatggcca acgagcgcgt ctggcccgcc atgtcgcaag ccttcgagac cgcccgaggc
     2641 gacctcgccg aacgcatgct cgccgccctc gaagccgccg aagccgccgg cggcgacatc
     2701 cgcggcaaac aatccgccgc cctcgtcgtc gtctccgcca aacccacggg ccgcccctgg
     2761 gccgaccgcg tcttcgacct gcgcatcgac gaccaccccc aacccctccc ggaactccgc
     2821 cgcctcgtca ccctccaacg cgcctacaac cacatgaacg cgggcgacct cgcggtcgaa
     2881 aagaaagaca acgaagcggc gctacgcgaa tactccgccg ccgaaaaact ggtccccgac
     2941 aatctggaga tgacctactg gcacgccatc gccctcgtca acatgaaccg caccgacgaa
     3001 gccttgccca tgttccgccg cgtcttccaa gccgacccca actggcgcac tttaaccacc
     3061 cgcctcccca aatccggcat cctccccgac gaccgaaaac tcctcgaccg catcactgct
     3121 gtcggcggga cgagataggc gaatcaggcg gaaaagagat cgacggaatc gacagggcta
     3181 acgggagcga gcagagacgc aggctgatgc gggttcgggc gcatgccgtg gtcgggccgc
     3241 gcctgacgtg cgggcgtcaa ttattaacgt accaatcgcc ggggtcgata cctgttgaag
     3301 ttcgcccggc gcgcaccgtt accttgacgg gcgcatggga agggcagttg accgacaggc
     3361 cgcagacgac gaattccgag tagtaagcct tataccctcg atagtccgca gtcggcgctt
     3421 cgccattcac gatggtggca aagacgtaat agctccccgc gggcacgcta agtctatacg
     3481 tcaccttacg actccttcga ttcgtagtgc gtttgtccgt gcaatggatg ctcctggaat
     3541 ccatcgcttc cgcacacacg atcattctcg gaaagtcgaa atcgctcggg taggacaacg
     3601 cgccggtgat gacacccgtt cgttgcacgg gcgtcttcga ttgaatggaa acaccgccgg
     3661 ttaatagtaa agtcacg
//
LOCUS       DBNGOH010000970         2908 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1613076, whole genome shotgun sequence.
ACCESSION   DBNGOH010000970 DBNGOH010000000
VERSION     DBNGOH010000970.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2908)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2908)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2908
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1613076"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1739
                     /locus_tag="AC159P_18925"
     CDS             <1..1739
                     /locus_tag="AC159P_18925"
                     /inference="COORDINATES: protein motif:HMM:NF016289.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HIR3697567.1"
                     /translation="VTAELIYIGDGSEESLKNLNVAGKIVLTSGSPFVAAQNAVYKRG
                     ALGVVTYETSENRDPLDFPDQIAWSRISINPPAGKKGTFAFVLPTRRGETLRRALTSG
                     EEEDFFATGKVIKGGRLVVKAKVDTEIGTAPGRTGFVEAIIRGTEPNKEQVVLTAHLQ
                     EEQGSANDDGSGCGNLLELARVFTKLIKEGKIKRPKRDLRFWWTDEIYSEYRYFGDHP
                     EETKKILANIHQDMTGANQAMGSRVQHLIFAPHSRTSYLDALFESVGTYLVHTNNGFL
                     AASRQGGLPRPHTRPLYATRGTRQGYNARFVPYFDSSDHMVFVEGAVGIPAVGLINWD
                     DPYIHSTDDDLFQIDQTQLKRNNFLIGAMALFLSTADDADVPVLTSETFAQGAKRLAN
                     DLQVAMSILRDSAGEPDKGWKHASFMVEQGVQREQRALLSALSFATNSRSGLQVHEMA
                     GRLKDKETALKAELQTFYAYLNGANPVAIQLSPAEQAASRKVPANAASLADYFANRNQ
                     VPGGARGKLHGLMRDEVFNFVDGRRSYYDIYKAVYAESMAAGTWYYGTVTLGDVVSLL
                     DAAVAAKALTLK"
     gene            2192..>2908
                     /locus_tag="AC159P_18930"
     CDS             2192..>2908
                     /locus_tag="AC159P_18930"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF024489.7"
                     /note="catalyzes the reversible transfer of the terminal
                     phosphate of ATP to form a long chain polyphosphate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA degradosome polyphosphate kinase"
                     /protein_id="HIR3697568.1"
                     /translation="MTTVTGNQPLPEDRRETSGAASMTPKPDDTKQRLFNRELSQLEF
                     YRRVLEEAEDATQPLLERLKFLAIFSSNVDEFFMIRVSGLKETLELNVRELSPDGMTQ
                     AGQLAAIRERLVPMVAEQARGLREDIMPQLRSHGIEIVAHDSLSADEQARMKEFFQSR
                     VFPVLTPQAVDPGHPFPYISNLSLNLALMVEPAPELGVTQSLTGKAEPRFVRVKVPTQ
                     VPRLVPVGEGATRFVLLEDLI"
BASE COUNT          546 a          973 c          940 g          449 t
ORIGIN      
        1 acgtgaccgc cgagctgatc tatatcggcg acggcagcga agagagtctg aagaacctga
       61 acgtcgccgg caagatcgtg ctcacgagcg gcagcccctt cgtggccgcg cagaacgcgg
      121 tctacaaacg cggcgcgctc ggcgtcgtca cctacgagac ctcggagaac agagacccgc
      181 tggacttccc cgaccagatc gcgtggtcgc gcatctcgat caacccgccg gcagggaaga
      241 agggcacgtt cgccttcgtg ctgccgacgc gcaggggcga gacgctgcgg cgcgcgctca
      301 cgtccggcga ggaggaagac ttcttcgcca ccggcaaggt catcaagggc gggcggctcg
      361 tcgtcaaggc gaaggtcgat accgagatcg ggacggcgcc gggccgcacc ggcttcgtcg
      421 aggccatcat ccgcggcacc gagccgaaca aggagcaggt cgtgctgacg gcgcacctgc
      481 aagaggagca aggctccgcg aacgacgacg ggagcggctg cggcaacctg ctggagctgg
      541 cgcgcgtctt caccaagctc atcaaggaag ggaagatcaa gcggcccaag cgcgacctgc
      601 gtttctggtg gacggacgaa atctattccg agtaccgcta cttcggcgac caccccgaag
      661 agacgaagaa gatactggcg aacatccatc aggacatgac cggcgcgaat caggcgatgg
      721 ggagccgcgt gcagcacctc atcttcgcgc cccactcgcg cacctcctac ctcgacgcgc
      781 tcttcgagag cgtcggcacc tacctcgttc acaccaacaa cggcttcctc gcggcgagcc
      841 gccagggggg cctcccgcgc ccgcacacgc gcccgctcta cgcgacgcgc ggcacgcggc
      901 agggctacaa cgcgcgcttc gtgccttact tcgactcgtc cgaccacatg gtcttcgtcg
      961 agggcgcggt cggcatcccc gccgtcgggc tgattaactg ggacgacccc tacatccact
     1021 cgaccgacga cgacctcttc cagatcgacc agacgcagct caagcgcaac aactttctta
     1081 tcggcgcgat ggcattgttc ctctccaccg cggacgatgc ggacgtgccc gtgctcacct
     1141 ccgagacttt cgcgcagggc gcgaagcgtc tcgccaacga cctgcaagtc gcgatgagca
     1201 tcctgcgcga ctcggcgggc gagccggaca agggttggaa acacgcgtcc ttcatggtcg
     1261 agcagggcgt tcagcgtgag cagcgtgcgc tgctctccgc gctgagcttc gccaccaaca
     1321 gccgctccgg tctacaggtg cacgagatgg ccgggcgttt gaaagataaa gagacggcgc
     1381 tgaaggccga gctgcaaacc ttctacgctt accttaacgg cgccaacccc gtcgccatcc
     1441 aactctcccc cgccgagcag gccgcgagca ggaaggtgcc ggccaacgcc gcgtcgctcg
     1501 cggactattt cgccaaccgc aatcaggtgc cgggcggcgc gcgcggcaag ctgcacgggc
     1561 tgatgcgcga cgaggtcttc aacttcgtgg acggcaggcg gagctactac gacatctaca
     1621 aggccgtcta cgcagagtcg atggccgcgg gcacctggta ctacggcacg gtcacgctcg
     1681 gcgatgtcgt cagcctgctc gacgcggcgg tggcggcgaa ggccctgacg ctgaagtgat
     1741 tcggcttcgg agggaacgga ttccgagaag cctgtggccg cccacgcttc tcagtaccgc
     1801 cggactcagg tgctttgctc ggagaaccga atgcgtacac gcggggcgtc acggagggtg
     1861 gcgcgtccga acgggacgag ggccgcaagt gttgaaatgc cggtgctttt caagagtgtg
     1921 ccgcgcggcg gggcgggagg aatggtcttt gcaagacggg cggggttgac cccttttcac
     1981 gatccgggcc gcgttagtgt ttgtgggggc gggtcttgaa gattctcgga aggagccgtg
     2041 accgaaaggt gacacccggc acgcgtctca agtggctgcg ccatcgccgc gccgccgcat
     2101 ctaacctgta aacccttctc ccctgccgga aaatcgagcc aagtgtgcgg gccgtactgt
     2161 agaatgtgcg gcacggagaa tatatagacc gatgacgact gtgaccggaa accaacccct
     2221 gcccgaagac cgccgcgaga cgagcggcgc cgcctctatg acccctaaac cggacgacac
     2281 caagcagcgc ctcttcaacc gcgagctgag ccagctcgaa ttctaccgcc gcgtgctgga
     2341 agaagcggag gacgcgacgc agcccctgct cgaacggctg aagttcctcg ccatcttctc
     2401 ctcgaacgtg gacgagttct tcatgatccg cgtctcgggg ctgaaggaga cgctggagct
     2461 gaacgtgcgc gagctgtcgc cggacggcat gacgcaggcc gggcagctcg ccgctatccg
     2521 cgagcggctg gtgccgatgg tcgccgagca ggcgcgcggc ctgcgcgaag acatcatgcc
     2581 gcagctccgt tcgcacggca tcgagatcgt cgcgcacgac tcgctgtcgg cggacgagca
     2641 ggcgcggatg aaagagttct tccagagccg cgtcttcccc gtgctcacgc cgcaggccgt
     2701 agaccccggc cacccgttcc cctacatctc gaacctcagt ctgaacctcg ccctgatggt
     2761 cgagccggcg cccgagctgg gcgtcacgca gtctttgacg ggcaaggccg agccgcgctt
     2821 cgtccgcgtc aaggtgccga cgcaggtgcc gcggctcgtc cccgtcggcg agggcgcgac
     2881 gcggttcgtc ctcctcgaag acctgatc
//
LOCUS       DBNGOH010000971         2433 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3996935, whole genome shotgun sequence.
ACCESSION   DBNGOH010000971 DBNGOH010000000
VERSION     DBNGOH010000971.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2433)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2433)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2433
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3996935"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1263
                     /locus_tag="AC159P_18935"
     CDS             <1..1263
                     /locus_tag="AC159P_18935"
                     /inference="COORDINATES: protein motif:HMM:NF013740.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HIR3697569.1"
                     /translation="AAEVAATEARARVVVRAATRTSMWLILFALILYYSIEGVIWVFK
                     ALTGVLLMIVLAIFFAYLIAPLVELVRGPFEVRGRERRLPRYAAIGLVYVVIFGSLAL
                     TTWLLLPRIGSQLDEFAKQAPAYLTNARTRAEGLNQIYERINLPPAVRKYANDQATKT
                     LDEIGGYIKEEGTTRAIHTLGYLPWLFLVPILAFFFLKDAESFRRSALSVLPQGRLRW
                     RGDEFFQDVNSTLAAYIRAQLIACLLIGTICTIGFVLIGVPYALVLGFAAGVLEFIPL
                     LGPLVVAVIAVSLASFASGSTAFAVLLFLGVLRVVHDYYTYPRIIGSGIHLHPLAVIL
                     AILAGHELAGLAGIFLAIPVIAILTVTYRHWLEHKGSQGLVADLLKPAEEAAEQDALA
                     AAGGRALGGDAGASAVESGQTRVAPTAQ"
     gene            1427..1831
                     /locus_tag="AC159P_18940"
     CDS             1427..1831
                     /locus_tag="AC159P_18940"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR3697570.1"
                     /translation="MPSEKRRVLCAEPHEDTCLLIKTLLGGQGHEVVTAQTVHDCLEL
                     ARRERFDLYMLDDDYADGTSIELCRELRALAPETPILFFSSLALPRDRQRALDAGALA
                     YLIKPDGLFDIVSTINSILLPRSPRHTSSERE"
     gene            1932..2303
                     /locus_tag="AC159P_18945"
     CDS             1932..2303
                     /locus_tag="AC159P_18945"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR3697571.1"
                     /translation="MPAEKRRILCAEANRDVGDLIVLMLEKKGYAVRAVQTVADCLKV
                     AAEERFDLYILNDTYIDGDSIELCRELRASNPLTPVLLFSLDSSGHNRRHAAATGARV
                     YVSKTSDFVSLVQTIDRLLHS"
BASE COUNT          390 a          890 c          720 g          433 t
ORIGIN      
        1 gcggcggagg tcgccgcgac cgaggcccgt gcgcgcgtcg tggtgcgcgc ggcgacgcgc
       61 acgtcgatgt ggctcatcct cttcgcgctc atcctctact actccatcga gggcgtgatc
      121 tgggtcttca aggcgctgac gggcgtgctg ctgatgatcg tgctggcgat cttcttcgcc
      181 tacctcatcg cgccgctcgt cgagctggtg cgcggcccct tcgaggtgcg cgggcgcgag
      241 cggcggctgc cgcgctacgc cgccatcggc ctcgtctacg tcgtcatctt cggctcgctg
      301 gcgctcacca cgtggcttct gctgccgcgc atcggcagcc agttggacga gttcgccaag
      361 caggcgcccg cctacctgac gaacgcgcgc acgcgcgccg aggggctgaa ccagatttac
      421 gagcgaatca acctcccgcc cgccgtgcgt aagtatgcca acgaccaagc cacgaaaacg
      481 ttggacgaga tcggcggtta tataaaggaa gagggcacca cccgcgccat ccacacgctt
      541 ggctacctgc catggctctt cctcgtcccg atcctcgcct tcttcttcct gaaggacgcc
      601 gagagcttcc gccgctcggc cctgagcgtg ctgccgcagg ggcgattgcg ctggcgcggc
      661 gacgagttct tccaggacgt gaactcgacc ctcgccgcct acatccgcgc gcagctcatc
      721 gcgtgcctgc tcatcggcac catctgcacc atcggcttcg tcctcatcgg cgtgccttac
      781 gcgctggtgc tcggcttcgc cgcgggcgtg ctcgaattca tcccgctgct cggcccgctc
      841 gtcgtggcgg tcatcgccgt ctctttggcg agcttcgcgt cgggcagcac ggccttcgcc
      901 gtcctcctct tcctcggcgt gctgcgcgtc gtgcacgact actacaccta cccgcgcatc
      961 atcggctcgg gcatccacct gcacccgctc gcggtcatcc tcgccatcct cgccggccac
     1021 gagctggcgg gactggccgg catcttcctc gccatcccgg tcatcgccat cctgacggtc
     1081 acctaccggc actggctcga acacaagggg agccaggggc tggtcgccga cctgctgaag
     1141 cccgccgagg aggccgccga gcaggacgcg ctggccgccg ccggggggcg tgcgctcggc
     1201 ggcgacgcgg gcgcgtcggc ggtcgaatcc ggacagacgc gcgtcgcgcc gacggcacaa
     1261 taaaactgtt gacctcccgc ccgaattcag gcttaatact ccccgttcga ggccgcccca
     1321 actaaccgga gtttccagta cgtctctgta cagacagccg acttaatcag agtttctgtc
     1381 gagtcacccg gagtttcctc ccgcggcgcg agaaggagtt tcccccatgc catccgagaa
     1441 gaggcgcgtt ttgtgcgccg aaccccatga agacacctgt ctcctcatca agacgctgct
     1501 cggcgggcag ggccacgagg tcgtcacggc ccagaccgtc cacgactgtc tggagctggc
     1561 gcggcgcgag cgcttcgacc tctacatgct cgacgacgac tacgcggacg gcaccagcat
     1621 cgagctgtgc cgcgagctgc gcgcgctcgc acccgagacg ccgatcctct tcttctcgtc
     1681 gctcgccttg ccgcgcgacc gccagcgcgc gctcgacgcc ggcgcgctcg cctacctcat
     1741 caagcccgac ggcctcttcg acatcgtgag cacgatcaac tccatcctcc tgccccgctc
     1801 cccgcgccac acgtcgagcg aacgcgagta agccttgacg cgcggagcgc cgccgattaa
     1861 agttgagcca cccccgagtt cccctctagt acgaacacac tctctttctc tgccagtcag
     1921 gggtgagacc gatgcctgcg gaaaagaggc gcatcctctg cgccgaagcg aaccgggacg
     1981 tgggcgacct gatcgtcctc atgctggaga agaagggcta cgcggtgcgg gcggtgcaga
     2041 cggtcgccga ctgtctgaag gtcgccgccg aagagcgctt cgacctctac atcctcaacg
     2101 acacctacat cgacggcgac agcatcgagc tgtgccgcga gctgcgcgcc tcgaacccgc
     2161 tgacaccggt cctgctcttc tcgctcgaca gctccggcca caaccgccgg cacgccgccg
     2221 ctacgggcgc ccgcgtctac gtcagcaaga cctccgactt cgtctcgctc gtccagacca
     2281 tcgaccggct gttacattcg taacagaagg caaaaggcaa agatacgggc agcgtgcttc
     2341 tcatcaacgt gatgagaagc acgctgcccg gcatcgtctg tcgaagacga gaagcagcgg
     2401 acctctcgtg ttcacttttg ccgtttgcct ctt
//
LOCUS       DBNGOH010000972         7093 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4911918, whole genome shotgun sequence.
ACCESSION   DBNGOH010000972 DBNGOH010000000
VERSION     DBNGOH010000972.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7093)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7093)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7093
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4911918"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(237..584)
                     /locus_tag="AC159P_18950"
     CDS             complement(237..584)
                     /locus_tag="AC159P_18950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697572.1"
                     /translation="MRWKLLIAASLLAATVGAGAVLAATYFALGAAPGEVPPAWLLAV
                     AVLAPLGATAYAAVFVYRHTARRRTLQAAATALASITLTLAALAAVPYLFAPRPSPPT
                     DVPPAKNVGQLDK"
     gene            718..1638
                     /locus_tag="AC159P_18955"
     CDS             718..1638
                     /locus_tag="AC159P_18955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002630629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HIR3697573.1"
                     /translation="MVTRNTLLYLSRHEGLKDFATRFSPFKKMTARFVAGEDIEETIA
                     AIRRLNAAGCTASFDHLNESVTQIAETEEEVREYLKILARIDETGVNSNVSIKLTQFG
                     LDIDAELAYRNARAVVEEAARRGNFVRVDMEDSRVTEVTLDIFRRLRAEFGLDDVGIV
                     VQSYLRRTYGDVQELLKIPARIRLCKGAYNEPPEVAFPDKKDVDDNYVRCMKVLLSSG
                     TYHGIATHDERMIGETVSHAQKEGIGKDAFEFQMLYGIRRDLEQKLVRDGYRLRIYVP
                     YGKHWYPYFMRRLAERPANVWFILKNLLKG"
     gene            1678..2193
                     /locus_tag="AC159P_18960"
     CDS             1678..2193
                     /locus_tag="AC159P_18960"
                     /inference="COORDINATES: protein motif:HMM:NF014062.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HIR3697574.1"
                     /translation="MGDNIAWVLWVVLGVVLIVAEIFTTGFVLLWFGVGALAAALAGL
                     IGFGYPFQFLIFFAVSIGLTAASRTILASLFAQPDQPDELKSGAASLPGQTGTVVTSS
                     HGALHEGAVKVFGSTWTAYPAEGEAPLEAGDRVVVERLQGASIYVRRVGAVPDWRARE
                     LGEGEKPEAGE"
     gene            complement(2292..3350)
                     /locus_tag="AC159P_18965"
     CDS             complement(2292..3350)
                     /locus_tag="AC159P_18965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cif family virulence factor"
                     /protein_id="HIR3697575.1"
                     /translation="MASLSRSVAAALAVSLSILGAAALAQDRRQARPAARGSASAPSP
                     LTGVYRIHIGDSDKLYSVVAGASSNLPFGEQQRFFIDLAVRLTPPDLLAIERRGSSIT
                     IGSSRAPRITLLADGVVRTERAGDGHLVRSRVALEGERLVFTSSGRTEDNFSVVFTPF
                     ENGKRLRVTRRLSAEQLTEPVIIQTVYDKISEQPRWDIYDETLLAKSEGDAPASDAGP
                     GRRPAGAERGEADALRGALTQWVEATNARDIERQMTFYMPELKAFYLARNAPRSAVRI
                     EKARVFAAAELIDIRAEEPEIVFRDAGRTAIMRFRKKYRIEDGSRSRRGEVVQELRWQ
                     RTRNGWKIFSERDVRVIR"
     gene            complement(3420..4349)
                     /locus_tag="AC159P_18970"
     CDS             complement(3420..4349)
                     /locus_tag="AC159P_18970"
                     /inference="COORDINATES: protein motif:HMM:NF018884.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HIR3697576.1"
                     /translation="MIVELHAKTHVGRVRAGNEDNYLVLDLATQASWTGTDGEEPPAE
                     VCRFEVGPKGIILAVSDGMGGALAGDVASRMAVETVRDLLTSGGEEGEAACGPGVPLA
                     ECLRNAIDYANFAIHRRSQEDPRCSGMGATLTGAAVTREGVDLVQVGDSRGYVLRSSQ
                     IKLATKDQSLVQQLVDVGQISEQEAETHMFRNVILQALGAQAELAPVTGRVRVFRDDL
                     LLLCSDGLSGKLRSEDMLNIIAEADGDLARACAELVAEANHRGGEDNITVILARFTGD
                     DLASPDSNRITIELPALDGDSTISRDDDTTENP"
     gene            complement(4427..5572)
                     /locus_tag="AC159P_18975"
     CDS             complement(4427..5572)
                     /locus_tag="AC159P_18975"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HIR3697577.1"
                     /translation="MGFSTTAIHAGNDPDPSTGSVTVPIYQTSTYKQDGLLRPHNGYE
                     YARTQNPTRSALEQNIAALEGGRFGYAFASGMSAIDATLRLVKAGEHVVVSDNTYGGT
                     FRLFSRVLADYGLEFTYVDTTEAANVEAALRETTRMVFIETPTNPVMMVTDMQAVSTI
                     AHRAGARVVCDNTFMSPYLQRPLEFGVDIVVHSTTKYLNGHSDSVGGAVVLNDEKDAE
                     RIQFLQNSVGAILSPFDSWLVLRGTKTLAVRMEQHDASGRKVAAFLEEHPKVKKLYYP
                     GSVSHRQHELAKRQQRGFGGMVAFDTGSLEGAKTVLEAVRLCTLAESLGGVETLISHP
                     ATMTHASVLPETRARIGITDGLVRISVGLEDVEDIIADLDQALDKIP"
     gene            complement(6033..6809)
                     /locus_tag="AC159P_18980"
     CDS             complement(6033..6809)
                     /locus_tag="AC159P_18980"
                     /inference="COORDINATES: protein motif:HMM:NF024821.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3697578.1"
                     /translation="MLWLILFFLTAAGSPTPAQIGHRQAPKDAGVLGDQDAASFEKGV
                     ELHGAGRYQEAIEAFERAVGLAPGNATAHNNLGTAYLDAGRYAESVAHFKRANSLRPD
                     FAEAYRNLGLAHYALARYEEAVASYRQALLLKPDFVAAYNDLGMAYNLSGKYREAIEA
                     HRQALLLDPLFADAYNNLGCVYLNVGKHGKAVEMFEHAIRLNPNSAEARFNLGVAYLA
                     LRDRRAAKEQLGLLHALDSGLAKRLSLRIFAGKVLDVGRQ"
BASE COUNT         1176 a         2262 c         2427 g         1228 t
ORIGIN      
        1 aaggacaagg cgttcgcacg caccgtcaac cgcgacgaca tacggcaggg cgtcgaggag
       61 ctgggcgtcg agctggacga acacgtccgc ttcgtcatcg aggcgctgaa gcccgtgcag
      121 agagagatcg gactgaaggg aaatgatgaa tgatgaatgc ggaatgatga atgaaaggca
      181 cggtgcctgt tatcattcaa cttgtcttct aatcatcgtt catcattccg cattcatcat
      241 ttatcgagtt gtccgacgtt cttcgcgggc ggcacgtcgg ttgggggcga ggggcgcggc
      301 gcgaagaggt aggggacggc ggcgagcgcg gcgagcgtga gcgtgatgga tgcaagcgcg
      361 gtggccgcgg cctggagggt gcggcggcgg gccgtgtggc ggtagacgaa gaccgcggcg
      421 taggctgtgg ccccgagcgg ggcgaggacg gcgacggcga ggagccacgc gggcggcact
      481 tcgccaggtg ccgcgccgag cgcgaagtag gtggcggcca gcaccgcgcc cgcgccgacc
      541 gtggcggcca gcagagaggc ggcgatcagc agcttccagc gcatgcagtg tcagtcttaa
      601 ggagacggtc gggtcgcggc gaggtgaaag ggcggcagga gcataccaca cgcccggcgg
      661 cgcacgcgcc cacatttttt cgggatttta tccaccggca gggttcgcaa accgaccatg
      721 gtcacgagaa acacactgct ctacctttcg cgccacgagg gcctgaagga cttcgccacg
      781 cgcttcagcc ccttcaagaa gatgacggcg cgcttcgtcg cgggcgagga catcgaggag
      841 accatcgccg ccatccgccg gctcaacgcc gccggctgca cggcctcgtt cgaccacctc
      901 aacgagtcgg tgacgcagat cgcggagacc gaggaggagg tgcgcgagta cctcaagatt
      961 ctcgcgcgca tcgacgagac gggcgtcaac tcgaacgtct cgatcaagct cacgcagttc
     1021 ggcctcgaca tcgacgcgga gctggcctac cgcaacgcgc gcgccgtcgt cgaagaggcc
     1081 gcgcgccgcg gcaacttcgt ccgcgtggac atggaagact cgcgcgtcac agaggtcacg
     1141 ctcgacatct tccgccgcct gcgcgccgag ttcggcctcg acgatgtcgg catcgtcgtc
     1201 cagtcgtacc tgcgccgcac ctacggcgac gtgcaggagc tactgaagat tcccgcgcgc
     1261 atccgcctct gcaagggcgc gtacaacgag ccgcctgagg tcgccttccc tgataagaaa
     1321 gatgtcgatg acaactacgt gcgctgcatg aaggtgctgc tctcgagcgg cacctaccac
     1381 ggcatcgcca cgcacgacga gcggatgatc ggcgagaccg tcagccacgc gcagaaggaa
     1441 ggcatcggca aggacgcctt cgagttccag atgctctacg gcatccgccg cgacctcgaa
     1501 cagaagctcg tgcgcgacgg ctaccgcctg cgcatctacg tgccttacgg caagcactgg
     1561 tatccttact tcatgcggcg gctcgccgag cgcccggcga acgtctggtt catcctgaag
     1621 aacctgctga agggctgagg ccgtcggcca taactttgga gagcggagcg ttaaacgatg
     1681 ggcgacaaca tcgcttgggt cttgtgggtc gtcctcggcg tcgtcctcat cgtcgccgaa
     1741 atcttcacga ccggcttcgt gctcctgtgg ttcggcgtcg gcgcgctggc cgccgcgctc
     1801 gccggcctca tcggcttcgg ctacccgttc cagttcctca tcttcttcgc cgtctccatc
     1861 ggcctcaccg ccgcctcgcg caccatcctc gccagcctct tcgcgcaacc cgaccagccc
     1921 gacgaactca agagcggcgc cgcctcgctc cccggccaga ccggcaccgt cgtcacctcc
     1981 agccacggcg ccctccacga aggcgccgtc aaagtcttcg gctccacctg gacggcctac
     2041 cccgccgaag gcgaagcccc gctcgaagcc ggcgaccgcg tcgtcgtcga acgcctgcaa
     2101 ggtgcctcga tctacgtccg aagggtgggg gccgtgcccg attggcgcgc gcgtgaattg
     2161 ggggaaggtg aaaagccgga ggcgggcgag tagcggggcc ttggtcgagt gaggtcgttg
     2221 ataaagtcgc gcgcttccat tttcggactg cgacgtgaag gcgcgtgggc gcggcgtcct
     2281 cagtcgccgc ttcagcggat gactctcacg tcgcgctcgc tgaaaatttt ccaaccgtta
     2341 cgcgtccgtt gccaacgcaa ctcctgcacg acctcgccgc ggcggctccg cgagccgtct
     2401 tcgatccgat actttttgcg gaagcgcatg atggccgtgc gccccgcgtc gcggaagacg
     2461 atctccggct cttcggcgcg gatgtcgatc agctccgccg ccgcgaagac gcgcgccttc
     2521 tcgatacgca cggcggagcg cggggcgttg cgggcgaggt agaaggcttt cagctccggc
     2581 atgtagaagg tcatctgccg ttcgatgtcg cgcgcgttcg tggcctccac ccactgcgtg
     2641 agcgcgccgc gcagcgcgtc ggcttcgccg cgctcggcgc cggcgggccg tcgtcccggc
     2701 ccggcgtcgg atgccggcgc gtcgccctcg cttttcgcca gcagcgtctc gtcgtagatg
     2761 tcccagcgcg gctgttcgga aatcttgtcg tagacggtct ggatgatgac cggctcggtg
     2821 agctgttcgg cggagaggcg gcgcgtgacg cgcagccgtt tgccgttctc gaagggcgtg
     2881 aagacgacgc tgaaattgtc ttccgtcctg ccgctggagg tgaagaccag ccgctcgcct
     2941 tcgagcgcga cgcgcgagcg gacgaggtgg ccgtcgcccg cgcgctcggt gcgtacgacg
     3001 ccgtcggcca ggagcgtgat gcgcggcgcg cgcgacgagc cgatggtgat gctggagccg
     3061 cggcgctcga tggccaggag gtcgggcggc gtgaggcgca ccgcgaggtc gatgaagaag
     3121 cgctgctgct cgccgaaggg gaggttgctc gaagcgcccg cgacgaccga atagagcttg
     3181 tcgctgtcgc cgatgtggat gcggtagacg cccgtcagcg gcgacggcgc ggacgcggag
     3241 ccgcgcgcgg cgggccgagc ttgtcgcctg tcctgagcga gcgccgccgc gccgagaatc
     3301 gagagggaga ccgccagggc cgccgcgaca cttctactta agcttgccat ttcgcccacc
     3361 ttgttccgga agtcgatacc cgccgccccg tgaaatcgtc gggcgcggcg cgagggggtt
     3421 caagggttct cggtcgtgtc gtcgtcgcgg ctgatggtgg agtcgccgtc gagggcgggc
     3481 agctcaatgg tgatgcggtt ggagtcgggg gaggcgaggt cgtcgccggt gaagcgggcg
     3541 aggatgacgg tgatgttgtc ttcgccgccg cggtggttgg cctcggcgac cagttcggcg
     3601 caggcgcggg cgaggtcgcc gtccgcctcg gcgatgatgt tgagcatgtc ctcggagcgg
     3661 agcttgccgg agaggccgtc gctacagagc agcaggaggt cgtcgcggaa gacgcggacg
     3721 cggcccgtga cgggcgccag ctcggcctgc gcccccaacg cctggaggat gacgttgcgg
     3781 aacatgtgcg tctcggcctc ctgctcgctg atctggccga catcgacgag ctgttggacg
     3841 agcgactggt ctttggtggc gagcttgatc tggctgctcc tgagcacgta gccgcggctg
     3901 tcgccgacct gcacgagatc gacgccctcg cgcgtgacgg cggcgccggt caaggtcgcg
     3961 cccatgccgc tgcaacgggg gtcttcctgg ctgcggcggt ggatggcgaa gttggcgtag
     4021 tcgatggcgt tgcggaggca ctcggccagg ggcacgcccg gcccgcacgc ggcctcgccc
     4081 tcttcgccgc ccgaggtcag caggtcgcgc acggtctcga ccgccatccg gctcgccacg
     4141 tcgcccgcga gcgcgccgcc catcccgtcc gagacggcga ggatgatgcc cttcggcccg
     4201 acctcgaagc ggcacacctc ggcgggcggc tcctcgccgt cggtgcccgt ccacgacgcc
     4261 tgcgtggcga ggtcgagcac gaggtagttg tcctcgttgc ccgcgcggac gcgcccaacg
     4321 tgagtcttgg cgtgcagctc tacgatcata agagacgtaa accgtaaacc gtaaaccgtg
     4381 acaagtcgga accgctttcc actcgtcacg gtttacggtt tacctgtcac gggattttgt
     4441 cgagcgcctg gtcgaggtcg gcgatgatgt cttcgacatc ttcgaggccg accgagatgc
     4501 ggacgagtcc gtcggtgatg ccgatgcgcg cgcgcgtctc gggcaagacc gaggcgtgcg
     4561 tcatcgtcgc cgggtgcgag atcagcgtct cgacgccgcc gaggctctcg gcgagcgtgc
     4621 acaggcgcac cgcttcgagc accgtcttcg cgccttccag agagccggtg tcgaaggcga
     4681 ccatcccgcc gaaaccccgc tgctgccgct tcgccagctc gtgctggcgg tgcgagacgg
     4741 agccggggta gtagagcttc ttcaccttcg ggtgctcttc gaggaaggcc gccaccttgc
     4801 gccccgaggc gtcgtgctgc tccatcctca cggcgagcgt cttcgtgccg cgcaaaacga
     4861 gccacgagtc gaacggcgac aggatcgcgc cgacgctgtt ctgtaaaaac tgaatccgct
     4921 cggcatcctt ttcgtcgttc agcacgacgg cgccgccgac gctgtccgag tggccgttca
     4981 gatacttcgt ggtcgagtgg acgacgatgt ccacgccgaa ctccagcggg cgttgcaggt
     5041 agggcgacat gaaggtgttg tcgcagacga cgcgcgcgcc cgcgcggtgc gcgattgtag
     5101 agacggcctg catgtccgtc accatcatca cggggttggt cggcgtctcg atgaagacca
     5161 tccgcgtcgt ctcgcgcagc gcggcctcga cgttcgcggc ctcggtcgtg tccacgtagg
     5221 tgaactcaag gccgtaatcg gccagcacgc ggctgaagag gcggaacgtc ccgccgtagg
     5281 tgttgtccga gacgacgacg tgctcgcccg ccttgacgag ccgcaaggtc gcgtcgatgg
     5341 ccgacatccc cgaggcgaag gcgtagccga acctgccgcc ttcgagcgcc gcgatgttct
     5401 gctccagcgc cgagcgcgtc gggttctgcg tgcgcgcgta ctcgtagccg ttgtggggcc
     5461 ggagcagccc gtcctgcttg taggtcgagg tctggtagat gggtaccgtc accgagccgg
     5521 tcgaagggtc ggggtcgttg cccgcgtgga tggccgtcgt cgagaatccc ataagcgaaa
     5581 gtccgcggct ccgaagtccg ggcctcaaag ctcaagtcga aagccgcgcg cctgaactct
     5641 gcaccatgaa ctcggaagaa tgccgcatgg tagcctagtg aagtgacgag tgacaagtgg
     5701 cgaacaaaag acgcgtgccg cgagaagcga agccgacccg gcgccgcgtg acaaaacctc
     5761 tcgacgcgcg ggcgaaggct ggcggactac tgtgccggag ttgaccgggg tgctgtaccc
     5821 ctggggtatg cggcctggtg aggaaaggcc cgggcaagtg cttcgtcagg acgatccaga
     5881 acggcggaca aggacgaggg ccgttccgcc taccgccgca acgcctctgt catgcggcgc
     5941 ggagcttcga agccgggtag ccgcagaaaa ataacgcccc ggctgctcgc gttgaaggca
     6001 ggcaagatgc cggggcttac tgttgcagtg cttcattgtc gccccacgtc aagaacctta
     6061 cccgcgaaga tccttaagga cagccttttg gctaagcccg agtcgagcgc atggagcaag
     6121 ccgagttgtt cctttgccgc ccgtctatcc cggagggcga ggtaggcgac gcccaggtta
     6181 aagcgcgcct cggctgagtt cgggttgagt ctgatggcat gctcgaacat ctctacggcc
     6241 tttccatgct tgcccacgtt gaggtagacg cagcccaggt tgttataagc gtcggcgaag
     6301 agcggatcga ggaggagggc ctgccgatgg gcctctatcg cctccctgta cttacctgaa
     6361 aggttatacg ccatgccgag gtcgttgtag gcggccacga agtccggctt caggaggagc
     6421 gcctgccggt aagaagcgac cgcctcctcg tagcgcgcca gggcgtaatg agccaaaccc
     6481 aggttgcgat atgcttcggc gaagtccggc ctgaggctgt tcgcccgctt gaagtgggcg
     6541 actgattccg catagcgccc cgcgtcgagg taggcggtgc ccaggttgtt gtgtgcggtc
     6601 gcgttgccgg gcgcgaggcc gaccgccctc tcgaaggcct ctatggcctc ctgataccgg
     6661 cctgccccgt gaagctctac gcctttctcg aaagatgccg catcctggtc gccgagcact
     6721 cctgcgtctt tcggtgcctg acggtgtccg atctgtgccg gggtcggaga accggcggca
     6781 gtgaggaaga acaggattaa ccagagcatg gttgtttgtc tcggttgtga catcagcggc
     6841 tcctccgggc taagcacgaa accctgtgtg cgcagtgcac gggcttaccg atgggggctt
     6901 aaccgaaaaa tcaagaggcc gtcgtcgcgg atgaggtatc cgcgacgacg gcctccgagt
     6961 gcttcggtca gccggaatgt ttgcttggca ggttccgcga cttagggcgc gaacttgacc
     7021 ttcttcttga ctgcttcggc catcaccggc aggccgtcct gaatcttcag cagctcgatc
     7081 tcgccgtgcc cca
//
LOCUS       DBNGOH010000973         2038 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4361818, whole genome shotgun sequence.
ACCESSION   DBNGOH010000973 DBNGOH010000000
VERSION     DBNGOH010000973.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2038)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4361818"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1978
                     /locus_tag="AC159P_18985"
     CDS             <1..1978
                     /locus_tag="AC159P_18985"
                     /inference="COORDINATES: protein motif:HMM:NF015140.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HIR3697579.1"
                     /translation="VAQVFIMLGDGTGSFGTPVGFASLLIPGPLVVFDFDNDGKLDIG
                     AALFGRFTIDSGGIVVMLGDGAGNFHTPRGQAAGTNPSDIALGRFNGDGVTDFAVANQ
                     HGGSITIFTPDNAGTFPIPSQTFGVGGGFGPLVESVAVGDFNGDGKDDVAAGVTFSTT
                     AAAPVVAVALGDGAGGLGPMTHHVVGSTNPSVATGDFNGDGHLDIVSANRGFDRSNVS
                     VLLGDGAGSFAPAQNFAGGSLPVFALPGDFDNDGRLDLAVTNSAGHDVSLIYNPCPGA
                     AAAPAVEFGAAHYAVGEGAGSLRVNVLRSGDASVAFTVRYASGGGTASERSDYTSAQG
                     ILSFAAGEARKSFSVVVTDDAFDEADETLNLTLSSPTGGVTLGPVSVADITIGDNDTG
                     PPASNPADGSDFFVRQHYADFLNREPDAAGLVFWSSQIEGCGADARCREVRRVNVSAA
                     FFLSIEFQETGYYAYRVHKAAFGDLPNQPVPVRFGDFLSDSRQIGDGVQVGVGNWQQQ
                     LDANKSAFTLAFVNRPEFAARYPSAMEPGAFVDALNVNTGGALSEAERDALVAELAAN
                     NTAAGRATIFRKVAEDQTLAEAQKNKAFVLMQYFGYLRRNPDDPDFQNNPDPNFLGYN
                     FWLQKLNQFDGNFVNAEMVRAFISSIEYRRRFGQ"
BASE COUNT          325 a          706 c          672 g          335 t
ORIGIN      
        1 cgtcgcgcaa gtcttcatca tgctcggcga cggcacgggg agtttcggca cgccggtcgg
       61 tttcgcctcg ttgctcatcc ccggcccgct cgtcgtcttc gatttcgaca acgacggcaa
      121 gttggacatc ggcgcggcgc tgttcggcag gttcacgata gattcgggcg gcatcgtcgt
      181 catgctcggc gacggggcgg gtaatttcca cacgccgcgc gggcaggcgg cgggcaccaa
      241 cccctctgac atcgcgctcg ggcgtttcaa cggcgacggc gtcacggact tcgccgtcgc
      301 caaccagcac ggcggcagca tcaccatctt cacccccgat aacgcgggca cgttccccat
      361 cccctcgcag accttcggcg tcggcggcgg cttcggcccg ctggtcgagt cggtcgcggt
      421 cggtgatttc aacggggacg ggaaggacga cgtggccgcg ggcgtcacct tctcgacgac
      481 cgccgccgcg ccggtcgtgg ccgtggcgct cggcgacggg gccggcggcc tcggccccat
      541 gacgcaccac gtcgtcgggt ctacgaaccc ttcggtggcg accggagact ttaacggcga
      601 cggccatctg gacatcgtca gcgcgaaccg cggctttgac aggagcaatg tctcggtgct
      661 gctcggcgac ggcgccggaa gcttcgcgcc ggcgcagaat ttcgcgggcg gcagcctgcc
      721 cgtgttcgcc ttgccgggag acttcgacaa cgacggcagg ctggacctgg ccgtcaccaa
      781 cagcgcgggc cacgacgtgt cgctcatcta taacccgtgt ccgggcgcgg cggcggcgcc
      841 cgccgtcgag ttcggcgcgg cgcactacgc cgtcggcgaa ggcgcgggca gtctccgcgt
      901 caacgtgctg cgctccggcg acgcctccgt ggccttcacc gtccgctacg cttcgggcgg
      961 cgggacggct tccgagcgct ccgactacac ttcggcgcag ggcattctga gcttcgccgc
     1021 gggcgaggcg cgcaagtcct tcagcgtggt cgtcaccgac gacgccttcg acgaggcgga
     1081 cgaaaccctc aacctcactc tgagcagccc gaccggcggc gtcacgctcg gcccggtcag
     1141 cgtcgcggac atcaccatcg gcgacaacga caccggtccc cccgcgtcga acccggcgga
     1201 cgggagcgac ttcttcgtcc gccagcacta cgcggatttc cttaaccgcg agcccgacgc
     1261 cgccggcctc gtcttctggt caagccagat cgaagggtgc ggggcggacg cgcggtgtcg
     1321 ggaggtgcga agggtcaacg tcagcgccgc cttcttcctc tcgatcgaat ttcaggagac
     1381 gggttattac gcctaccgcg tccacaaggc cgcgttcggc gacctcccga accagcccgt
     1441 ccccgtccgc ttcggcgact tcctctcgga ctcgcggcag atcggcgacg gcgtgcaggt
     1501 cggcgtcggc aactggcagc agcagttgga cgcgaacaag agtgcgttca cgctggcctt
     1561 cgtcaacagg ccggagttcg ccgcgcgcta cccttcggcg atggagccgg gcgcgttcgt
     1621 ggacgccctg aacgtcaaca ccggcggcgc gctctccgag gccgagcgcg acgcgctggt
     1681 cgcggagttg gcggcgaaca acacggcggc gggccgcgcg acaattttcc gcaaggtggc
     1741 cgaggatcaa acgctggcgg aagctcagaa gaacaaggcc ttcgtgctga tgcagtactt
     1801 cgggtatctc aggcgcaacc cggacgaccc ggacttccag aacaacccgg acccgaactt
     1861 cctcggctac aacttctggc ttcagaagct caaccagttc gacggcaact tcgtcaacgc
     1921 cgagatggtc agggccttta tctcctcgat tgagtatcgc cggcgcttcg gccagtaaag
     1981 tgagcggcga gcagtggaag aaaggggctt gagaggttcg ctctcaagcc cctttcta
//
LOCUS       DBNGOH010000974         6383 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_330718, whole genome shotgun sequence.
ACCESSION   DBNGOH010000974 DBNGOH010000000
VERSION     DBNGOH010000974.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6383)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6383)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6383
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_330718"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(207..1058)
                     /locus_tag="AC159P_18990"
     CDS             complement(207..1058)
                     /locus_tag="AC159P_18990"
                     /inference="COORDINATES: protein motif:HMM:NF013360.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016641 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, oxygen as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysyl oxidase family protein"
                     /protein_id="HIR3697580.1"
                     /translation="MKKRSLLIAISLCAALGVALLAYGVKRGSAQVSGPNLTVRSDVL
                     GSQWVVRDENIPAEACSAIEGGVTPGLRRLVRFTVMTPNLGDKDIVVGDPNEHVAAGD
                     GLFEFASCHGHYHFRHYALYQLIDPKSGKIWRAAKRGFCMLDTDPNPASLGGVPPGPG
                     KYRNCGAVGIPGNQGVSAGWADTYRFTLAGQYFVLDGGDGQDPVPPGDYVIRVTVNPP
                     FVAKKGEPCPHKDTNGFCHQLPETNYDDNVGQTSIRIPNHPGRQGVGPLAGNTEPAEE
                     CDKGCNQ"
     gene            1831..2037
                     /locus_tag="AC159P_18995"
     CDS             1831..2037
                     /locus_tag="AC159P_18995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697581.1"
                     /translation="MSIGISTAELPGVNIDDLQKRLRERLRILTQSMVGNSRDPEIRG
                     LRITPNVYTTTAELDRLVTALKAA"
     gene            2280..3560
                     /locus_tag="AC159P_19000"
     CDS             2280..3560
                     /locus_tag="AC159P_19000"
                     /inference="COORDINATES: protein motif:HMM:NF019912.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LVIVD repeat-containing protein"
                     /protein_id="HIR3697582.1"
                     /translation="MTLSAVCLCLAAVARGRAEQPAGFRNITLVGSLQLAPFNQKAHG
                     DVAAYKHLAFVGKWGSVCTGTGVDIIDISTPSTPVKVAATTGRPGTSTEDMKAIQIGT
                     RDVLAVGLQECGPDPAQGASGLELYDITEPNDPRLLSFFDVDALGADVHGIHELDLTT
                     TPSGRALALAAVPHLEVGTAGAGGLGGVGDLLIIDVTYPTKPTLVAEWGVLDEPGLGL
                     AFYESVRQGSSDRTFLHSARAGKDGTRAYLSYWDAGVIVLDITDPARPAYLGRTSFAP
                     ADEGNAHSVAEARGGNVLVQADEDTDPFAGDGEFNGWGYLRFFDISDPSNPVLLGTFA
                     TANANDEAVAAEGNWSAHNPEVRGNTVYASWYNDGVRVIDISATSAPREVASWTGAGT
                     PAGAPAVNIWGVAPHQNLLLASDRNYGLYILRVP"
     gene            3711..4079
                     /locus_tag="AC159P_19005"
     CDS             3711..4079
                     /locus_tag="AC159P_19005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697583.1"
                     /translation="MEPAAWLVLVPLAFASLLTGLVSSLGTTWGLFRHYWVVFKLLIN
                     VFATIVLLIFMETFRLMAAAAADPIADLGEVRSASPVVHAALALLLLLVATVLGVYKP
                     RGMTRYGWGKQQVQRKASQP"
     gene            4236..4733
                     /locus_tag="AC159P_19010"
     CDS             4236..4733
                     /locus_tag="AC159P_19010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697584.1"
                     /translation="MPASKLVLFCALAVTAHATAPSVPGQGPGARCTRYKGGSVKQKS
                     SKPREARTSHDLDKLVEALKAQGLGVERAGDVSQPFFSVESRALTVNGENVQVFQYRT
                     AEAAAKEASQVSPDGSGVGTSMASWVGTPHFFRGGRLIALYVGDDAKVLEALRASLGA
                     QFAGG"
     gene            4855..6150
                     /locus_tag="AC159P_19015"
     CDS             4855..6150
                     /locus_tag="AC159P_19015"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004308 - exo-alpha-sialidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HIR3697585.1"
                     /translation="MKVLPLARTLSLLAAAVFALGCGRNPPEPSQGDGKPNVPTATGP
                     ALEVREAESPSGPDSREPELFATAGGRVILSWVEKVGGKRYALRFTSRDAAGWAEPST
                     VAEGENWFVNWADFPSVIALADGSLAAHWLVKSGPGTYAYDVHVARSNDGGKTWGRPV
                     VPHTDGTRTEHGFVSLIPLADGRLGAVWVDGRATKDFKEGDEEKGPLPVSMELRYAAI
                     DAEGRLSDEAVLDERICECCQTSAALTSEGPIAAYCDRSEREVRDIHFVRQQPGGWSG
                     PRPVHADNWEINGCPVNGPSIAAEGRRVALAWFTGEGDSPRVQVAFSGDAGATFGGPT
                     RVDDGEALGRVDVVMLADGSALVCWMAGGPEGGANKVRRVAPDGTLGPVAIVARSDVS
                     RSSGYPRMARLGDTVHFAWTQFGKPSRVRTATADVSAFR"
BASE COUNT          994 a         2100 c         2231 g         1058 t
ORIGIN      
        1 cctccttggc ggtctcatgc gcaaactgag tctaccgcag ctcactcgtg agctagaggt
       61 gcctgcttca tcacatcaga accttcgtat ctaagagatt actcctcggc gttcgtggaa
      121 gttaccgaaa cgtaagaatg cgccacgtgt tccagagaca cgtggcgcat tcttattcta
      181 acgccggtgg aatccgggcg gcgcgtctac tggttgcagc ccttgtcgca ctcctcagcc
      241 ggctcggtgt tgccggccag ggggccgacg ccctggcggc cggggtggtt gggaatcctg
      301 atcgaagtct gcccgacgtt gtcgtcgtag ttagtctcgg gcaactggtg gcagaagccg
      361 ttggtgtctt tgtgggggca cggctcgccc ttcttggcga cgaagggcgg gttgacggtg
      421 acgcggatga cgtagtcgcc cggcggtacc gggtcttggc catcgccgcc gtcgaggacg
      481 aagtactggc cggcgagcgt gaagcggtag gtgtcggccc agccggccga gacgccctgg
      541 ttgccgggga tgccgaccgc gccgcagttg cggtacttgc cggggccggg cgggacgccg
      601 ccgagcgagg ccgggttcgg gtcggtgtcg agcatgcaga atccgcgctt ggcggcgcgc
      661 cagattttgc cgctcttcgg gtcgatgagc tggtagagcg cgtagtggcg gaagtggtag
      721 tggccgtggc acgaggcgaa ctcgaagagg ccgtcgcccg cggcgacgtg ctcgttgggg
      781 tcaccgacga cgatgtcctt gtcgccgagg ttcggagtca tcacggtgaa gcggacgagg
      841 cggcggagtc ccggcgtgac gccgccttct atggcgctgc aagcctcggc cgggatgttc
      901 tcgtcgcgca cgacccactg tgagccgagc acgtccgagc gcacggtgag gttggggccg
      961 ctgacctgcg ccgagccgcg cttgacgccg taggcgagca gggcgacgcc gagcgccgca
     1021 cacagagaga tggcgataag gagggaacgt tttttcacgg gttgctgacc tccgttaggc
     1081 taggctgctg tagttgtcga agccgcgtag tcgggcgtcg gcggagagct gggcggagcc
     1141 gggcgacagc cgtggggtgc gcactgccgc ccggcagacg taaacgtgga gaatcactcc
     1201 ggattggaaa caccggccgg agcaggttgc gtcacccgcc gccagaaaat tctgcgcgcg
     1261 tggcgtgaga ttacgttaga atttgtcgaa ctaccgttta gaccttgggg cgtcgctggc
     1321 aacatcgagc ttcgcctgcg tgtgaggcca gagagactat atccggcggc ggtgttagtc
     1381 aagcgccctg tcgctttcgg cacggggctc aggtgaaccc gccgggacgg cctcagcttc
     1441 gaggtggtgg tcgtcatgga cgaccgcgcg caaaggggag ttgagggtca gggcgccgaa
     1501 gctgccaggg caacgtcatc tgggcgagcg aggagcaccg gtaagcactt tcaggacgac
     1561 ggcctgactc cgttcttgga cggctcggtg tttcaggcga gttcgagggg cggggcaggt
     1621 cgtttgcccc gccccttcgt cttggggtag aacgcgcgcc gggtcggcgc agcacaccgg
     1681 cctccccggt agggctgccc gccctcgcgc ttcaccgctc gctcggcgcg gcgccttccg
     1741 cgccgcgccg gcgctacctt agtgcgcacc tgcgcgagcg ggtcgccgcc gcgctccccg
     1801 gcgcgcgttt acagtgtcga ggagcccgcg atgagcatag gcatctcgac ggccgagctt
     1861 cccggcgtga acatcgacga tctgcagaag cggctgcgcg agcgcctccg aatcctaacg
     1921 cagtctatgg tcggcaacag tcgcgacccg gagatccgcg ggctgcgtat cacgccgaac
     1981 gtctacacga cgaccgctga gctcgaccgg ctggttacgg cgctgaaggc tgcctagacc
     2041 gcgcgtccgc gacggcagct tcagacggca gagagttggc cgggcagccg ccgccgttga
     2101 cccacccccg acagtggcgt atcttctgac cactccgatc acgcgtcggt ctgaactcta
     2161 ggcttgcgcc cccgaccgca cccctcctgc ttcagtctct tgtttcggtc ttacggcccg
     2221 ccgctccgag aaaggggact cccgcgatgc cacgccctgc caaaccgctt ctcgcgttga
     2281 tgactctgtc ggccgtctgc ctgtgcctcg ccgccgtcgc gcgcggccgg gcggagcagc
     2341 ccgccggctt ccggaacatt acgttggtcg gctccctcca gctggccccc ttcaaccaga
     2401 aggcccacgg ggacgtggcg gcctacaagc acctcgcctt cgtcggcaag tggggcagcg
     2461 tctgcaccgg gacgggcgtg gacatcatcg acatctcgac cccgtcaaca ccggtgaagg
     2521 tcgccgccac gacgggtcga cccggcactt cgacggagga catgaaggcg atacagatcg
     2581 gcacgcggga cgtgctcgcc gtcgggctcc aggaatgcgg cccggacccc gcgcagggcg
     2641 cgagcgggct ggagttgtat gacatcaccg agcctaacga cccgcggctc ctgagctttt
     2701 tcgacgtcga cgcgctcggg gccgatgtgc acggaatcca cgagctggac ctgaccacga
     2761 ccccgagcgg gcgcgcgctg gcgctcgccg ccgtgcccca cctcgaggtc gggacggcgg
     2821 gcgccggcgg cctggggggc gtgggcgact tgctgatcat cgacgtgacc tacccgacca
     2881 agccgaccct cgtcgccgag tggggcgtgc tcgacgagcc tggtttgggc ctggccttct
     2941 acgagagcgt ccgccaagga agctcagaca ggacgttcct gcacagcgcg agggccggca
     3001 aggacggcac ccgcgcctac ctctcttact gggacgcggg cgtgatcgtg ctcgacatca
     3061 ccgacccggc ccgccccgcc tacctcgggc gcacctcgtt cgcgccggcc gacgagggca
     3121 acgcccactc ggtcgccgag gcccgtggcg gcaacgtcct cgtacaggcg gacgaggaca
     3181 cagacccctt cgccggcgac ggggagttca acggctgggg ctacctgcgg ttcttcgaca
     3241 tctccgaccc ctccaacccc gtgctgctgg gcaccttcgc gacggcgaac gcgaacgacg
     3301 aggcggtcgc agcggagggc aactggagcg cgcacaaccc cgaggtgcgg ggcaacacgg
     3361 tgtacgcctc ctggtacaac gacggggtgc gcgtgatcga catctcggcg acctccgcgc
     3421 cgcgtgaggt cgcctcctgg accggcgccg ggacaccggc gggggcgccg gccgtcaaca
     3481 tctggggcgt cgccccccac cagaacctcc tgctggcgag cgaccgcaac tacggcctct
     3541 acatcctccg ggtgccgtga ccgcggacgc cgcccgtttg ttctgtgacc gcgcggcggg
     3601 agggcggacg aaatagagtc ggacctcagc gatccctcag cggactcatc tctgggcgtg
     3661 gtcgcgatcg gctccggcct cgtcgcgggc ggcgcggcgc ctacctcgtg atggagccgg
     3721 ccgcctggct cgtcctcgtc ccgttggcct tcgcctcgct gctgaccggg ctcgtttcgt
     3781 cactgggcac cacgtggggc ctgttccggc actactgggt cgttttcaaa ctcctgataa
     3841 acgtctttgc caccatcgtc ttgctgattt tcatggagac gtttcgcctc atggcggctg
     3901 cggcggcaga cccgattgcc gatctcggcg aggtgcgcag cgcctccccc gtggtccacg
     3961 ccgcgctcgc cctgctgctg ttgctcgtgg ccaccgtgct gggtgtgtac aagccgcggg
     4021 gcatgacccg gtacgggtgg ggtaagcagc aggtgcagcg taaggcgtcg cagccgtagg
     4081 ttcggcgacc cctgttcggc ctcgaccgaa cagagaaata cggcttcatt ttgttatttt
     4141 gaatgtcggg agggtgcgga cgaggcccgc cggtgtttaa gccggccggc gtaacacgga
     4201 atcaccgccg gtgccggcgg taatgagggg aagcaatgcc cgccagtaaa ctcgtactgt
     4261 tttgcgccct cgcggtgacg gctcacgcca ccgccccttc ggttccaggt caggggccgg
     4321 gcgcccgctg cacgcgctac aagggcggct cggttaagca gaagtcttcg aagccacggg
     4381 aggcgcgaac gtcccacgac ctcgacaagc tggtcgaagc cctgaaggct cagggcttgg
     4441 gcgtcgagcg cgcgggcgac gtgtcacaac ccttcttctc cgtcgaaagc cgcgccctga
     4501 ccgtcaacgg cgagaacgtt caggtcttcc agtacaggac ggcggaagcg gccgcgaaag
     4561 aggcgagtca agtcagcccg gacggttcag gcgtgggcac cagcatggcg tcgtgggtcg
     4621 gcacgccgca cttcttcagg ggcgggagat tgattgccct ctacgtcggg gacgacgcga
     4681 aagtgttgga ggcgctccgc gcgtctctcg gggcgcagtt cgcaggcggg tagggtcagt
     4741 cgcggtaact attcgtccac cttcgagtgg atgccggcgt gcctctaaaa tacattgcct
     4801 gctacttctt aaaccgtcgt cgggcctgag ccgaaaagga gtcggtctca tcagatgaaa
     4861 gttttgcctt tggcgcgcac gctctcgctg ttggcagccg cagtcttcgc cctcggctgc
     4921 ggccggaacc ctcccgagcc gtcccaaggc gatggcaagc ccaacgtccc caccgcgaca
     4981 ggaccggcgc tcgaggtgcg cgaagccgag tcgccgtcgg gcccggacag ccgcgagccc
     5041 gaactcttcg ccaccgccgg cggccgggtc atcctgagct gggtcgagaa ggtcgggggg
     5101 aagcgctacg cgctgcgctt cacgtcgcgc gacgccgcgg gctgggcgga gccgagcacc
     5161 gtcgccgagg gcgagaactg gttcgtcaac tgggcggact tcccgtcggt catcgcactc
     5221 gcggacggct ctctggccgc gcactggctg gtcaagagcg ggccgggcac ctacgcctac
     5281 gacgtacacg tcgcgcgctc gaacgacggc ggtaagactt gggggcggcc cgtcgtcccg
     5341 cacacggacg gcacgcggac cgagcacggc ttcgtctcgc tgatcccgct cgccgacggg
     5401 cgcctcgggg cggtgtgggt agacggacgc gccaccaagg acttcaagga gggggacgag
     5461 gagaaaggcc cgctccccgt cagcatggag ctgcgctacg cggccatcga cgccgaaggc
     5521 cgcctctctg acgaggccgt gctcgacgag cgcatctgcg agtgctgtca gacctcggcg
     5581 gcgctcacgt ccgaggggcc catagcagcc tactgcgacc gctcggagcg agaggtgcgg
     5641 gacatccact tcgtacgtca acagcccgga ggttggagcg gcccgcggcc cgtgcacgcc
     5701 gacaactggg agatcaacgg ctgcccggtc aacgggccgt caatcgccgc ggagggacgc
     5761 cgcgtggcgc tcgcctggtt cacgggcgag ggggactcgc cgcgcgtcca agtggcgttc
     5821 tccggcgacg cgggcgcgac tttcggcggc ccgacgcgcg tggatgacgg cgaggccttg
     5881 gggcgggtcg acgtcgtgat gctggcggac ggctcggcgc tggtctgctg gatggcgggc
     5941 ggcccggaag gcggagccaa caaggtgcga cgcgtcgcgc ccgacggcac tctcggaccc
     6001 gtggcgatcg tcgcccggtc tgacgtctcg cgctcgagcg gctacccccg catggcccgc
     6061 ctcggcgaca ccgtacattt cgcgtggacg cagttcggta agccttcgcg ggtgcgcacg
     6121 gcgaccgccg acgtgagcgc cttcaggtga gacgtaccac gccgccgcgt tcggtcgcgg
     6181 ataatgaatc tcgcgccgtg gaaaattctc gcttgactct gtacccgggt acagggtgta
     6241 taaattctca ggcgataggg aaggtgaacc gcccggcgga ctccgcggcg gggccgtaca
     6301 tcataagtca tggtgacgag aacgacgcgc tgacggcgct cgaccctgct ggactcgccg
     6361 gtgggtctgt aataggatta cgc
//
LOCUS       DBNGOH010000975         4130 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3263582, whole genome shotgun sequence.
ACCESSION   DBNGOH010000975 DBNGOH010000000
VERSION     DBNGOH010000975.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4130)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4130)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4130
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3263582"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1462)
                     /locus_tag="AC159P_19020"
     CDS             complement(<1..1462)
                     /locus_tag="AC159P_19020"
                     /inference="COORDINATES: protein motif:HMM:NF013700.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HIR3697586.1"
                     /translation="MTGSTSTQISITKTKGRSSARITRLINFALAGAVILCAAGTAAA
                     QNEKSKGVYRIPYENGTNVKVTRDHTKHTPVGRLDMVGTGGSGEYKIVAAADGTIRFI
                     EDGFSAQVQNDDDDETPNKPCTNNYVWIEHANGEWTKYSHMQKDSSTKKAKLKVGQFV
                     KAGTYLGDEGKVGCASGDHLHFEVGVPRATNPVSEVGGFLADNSGSKRNRAPRICNIS
                     GGLFKSGETYTAENVPGNLKPGSAEVARHGLPAEDYQCLFDQAVNADYRLEWVDGFSV
                     NGKLYFNVIYRPDKTGNWTASHNLTGAQYQDVFDKRKAAGFRLKQVDSYPVGNQIRYA
                     AIFVKDGAAVAAYHGLSAADHQKKFDELTAGPWKPRNISVVSLGGDRFYTALYEKTNT
                     GSSQAKSFLSAAEYQQLSDENKQKGRQLAYLNVYEHRGQPRFSAIWNSATQGAFKARH
                     GLSGGAYQQEWEDALKGGLLTRAVTGYEDGNAIRYAA"
     gene            complement(1538..4048)
                     /locus_tag="AC159P_19025"
     CDS             complement(1538..4048)
                     /locus_tag="AC159P_19025"
                     /inference="COORDINATES: protein motif:HMM:NF025006.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HIR3697587.1"
                     /translation="MRSSKCSKRSPLVVLGVALIIVCLGLRGGAGATRAAQGQVVTVG
                     VVAFQDESGAEVAPGLVGQLARTLQQKLAATHKDLLPRAVGAEPGAAPAGTLGVEQLA
                     AFGKQRGLKFVVRGGVLAVETSGAGAAVVAQVYAEVVSVEDGELRGVRAEGGGADAGA
                     ALNAAVEKLAAEVYRAVVSPSGDAADGAQAPPPSGESDPEAPAPSSADEGAAPATAET
                     QGESDAEAAGAVEDDEALQQLIAQAESLAASGAGSADSLGALNRALEGLQRALAAKAA
                     RLEQAQDTARADADIASRRQELEGAVSRLTEEAAAAGTTDAGDGAAEGEQTSGEKKKL
                     TTSINDFLGESLSILQKIQEMRALLRGAGEEQSSAGGDVGAGGEDGDAGGASAPAEEA
                     TEEVSGVVTEGGEPVEGLSVTEAESGRSTTTGADGTYTLKGVPAGRLTNLVLTKAGAR
                     VATARLDLPRGRSAVADFELKAKAGARAAASPLRVVPSTVFVKASKAGAAAGAGTLKG
                     TVRGANGQPLARALVTLHQLAVARTDSAGRYTFLNVPAGAHQLTIYRSGLRAKTERVH
                     VTAKKSSESRVAFASGDKTDAARTGGRLLVRGAGTVLRGSVLGQDDRPLAGAKVSVVE
                     TGAQGGGAVAALANRQGGFELRDLKPGSYRVLVSKAGYEPVAQSVSLTAGASRPFEFR
                     LKRMRAPFLDRALEARNVSRANRPAAEPRRAAPQAATPGGNRPGERRAALVGSTRPQP
                     PAAVSNTGQLSGLVVDAKTGRPVAGAAVSVAGLGRATTNRAGSFFFPQVAPGTHRINV
                     RLKGYGDAVATANVRGGQRATLSLRLLPAVAPARIGIR"
BASE COUNT          513 a         1474 c         1418 g          725 t
ORIGIN      
        1 cggcggcgta gcggatcgca ttcccatcct cgtagcccgt cacggcccgt gtcagcagcc
       61 cgcccttcag cgcgtcctcc cactcctgct ggtaggcgcc tccgctcagg ccgtgccgcg
      121 ccttgaaggc gccctgcgtg gcggagttcc agatggcgga gaagcggggc tggccccggt
      181 gctcgtacac gttgaggtag gcgagctggc ggcccttctg cttgttctcg tcggagagtt
      241 gctgatactc cgccgcggag aggaacgact tggcctgcga gctgcccgtg ttggtcttct
      301 cgtagagcgc cgtgtagaag cggtcgccgc ccagcgagac gaccgagatg ttcctcggct
      361 tccacgggcc ggcggtcagc tcgtcgaact tcttctggtg atcggcggcg gagaggccgt
      421 ggtaggccgc gaccgcggcg ccgtccttga cgaagatcgc ggcgtagcga atctggttgc
      481 cgaccgggta gctgtccacc tgcttaaggc ggaagccggc ggccttgcgc ttgtcgaaca
      541 cgtcctgata ctgcgcgccc gtcaggttgt gcgacgccgt ccagttcccg gtcttgtcgg
      601 gccggtagat gacgttgaag tagagcttgc cgttgacgct gaagccgtca acccattcga
      661 ggcggtagtc ggcgttgacg gcctgatcga acaggcactg gtagtcctcc gccggcaggc
      721 cgtggcgcgc gacctcggcg gaacccggct tcaggttgcc gggcacgttc tccgccgtgt
      781 aggtctcgcc gctcttgaag aggccgcccg agatgttgca gatgcgcggg gcgcggttgc
      841 gcttgctgcc ggaattatcc gcgaggaagc cgccgacctc cgagacggga ttggtggcgc
      901 gcggcacgcc gacctcgaag tggaggtggt cgccgctggc gcaccccacc ttgccctcgt
      961 cgccgaggta ggtgcccgcc ttgacgaact gcccgacctt gagcttggcc ttcttggtcg
     1021 aagagtcttt ctgcatatgc gaatacttcg tccactcgcc gttggcgtgc tcgatccaga
     1081 catagttgtt ggtgcagggc ttgttcggcg tctcgtcgtc gtcgtcgttc tgcacctgcg
     1141 cgctgaagcc gtcctcgatg aaacggatgg tgccgtcagc cgccgccacg atcttgtact
     1201 cgccgctgcc gcccgtcccg accatgtcga gccggcccac gggcgtgtgc ttggtgtggt
     1261 cgcgcgtgac cttgacgttg gtgccgttct cgtaggggat acgatagacg cccttcgact
     1321 tttcgttctg ggcggcggct gtgcccgcgg cgcagaggat gaccgccccg gccagtgcga
     1381 aattgatgag ccttgtgatg cgtgcgctcg acctgccttt cgtcttcgtg atggaaattt
     1441 gcgtgctcgt cgatccggtc atttctttct ctccttggag gactgcgatc aatgttgcga
     1501 gtgtgattgg cgactaccgc tcttcgccga gcggacttca acgaattccg atgcgtgcgg
     1561 gcgcgactgc gggaagcagg cggaggctca gcgtggccct ctgcccgccg cggacgttag
     1621 ccgtcgcgac cgcatcgccg tagcccttca gccgcacgtt gatgcggtgc gtgccgggcg
     1681 cgacctgcgg gaagaaaaaa ctaccggcgc ggttcgtcgt cgcgcggccc agacccgcga
     1741 ccgagacggc ggcgccggcc acgggtcggc ccgtcttcgc gtcaacgacc agcccgctca
     1801 gttgacctgt gttcgatacc gccgccgggg gctgcggtct ggtagacccg acgagggccg
     1861 cgcgccgttc gcccggtcgg ttgccgccgg gcgtcgccgc ctgcggcgcg gcgcggcgcg
     1921 gctccgcggc gggcctgttc gcgcggctga cgtttcgcgc ttcgagcgcg cggtcgagga
     1981 agggcgcgcg catccgcttc agacgaaact cgaacggacg ggatgcgccc gccgtgagcg
     2041 agacgctttg cgcgaccggc tcgtacccgg ccttcgagac gagcacgcgg tatgaccccg
     2101 gcttgaggtc gcgcagctcg aagcctccct gccgattcgc cagcgccgcg acggcgccgc
     2161 caccctgcgc gcccgtctcc acgaccgaca ccttcgcccc ggccagcggg cggtcgtcct
     2221 gaccgaggac gctgccgcgc aagaccgtgc cggcgccgcg gacgagcaag cgcccgcccg
     2281 tccgtgcggc gtcggtcttg tcgccggacg cgaatgccac ccgcgactcg ctgctcttct
     2341 tcgcggtcac atgaacccgc tcggtcttcg cgcgcaggcc gctccggtag atcgtcagtt
     2401 ggtgcgcgcc cgccgggacg ttgagaaagg tgtagcgccc ggcggaatcg gttcgcgcca
     2461 cggcgagctg gtgcagcgtg acgagtgcgc gcgcgagcgg ctggccgttc gcgccgcgca
     2521 cggtgccctt gagcgtgccc gcccctgccg ccgcgcccgc tttcgacgcc ttgacgaaga
     2581 ccgtcgaggg cacgacgcgg agcggcgacg ccgcggcgcg cgcgcccgct ttggccttca
     2641 gctcgaagtc ggccacggct gagcgaccgc ggggcaggtc gagccgcgcc gtcgccactc
     2701 gtgcgcccgc tttcgtcagg acgaggttgg tgagccgacc cgccgggacg cccttcagcg
     2761 tgtaagtgcc gtcggctccg gtcgtggtgc tccggcccga ttcggcttcg gtaacgctga
     2821 gaccctcgac cggctcgcct ccctccgtga cgacgccgct gacctcttcg gtcgcttcct
     2881 cggcgggcgc cgaagcgccc cctgcgtcgc cgtcttcgcc gcccgcgcca acgtcgccgc
     2941 ccgcggaaga ctgttcctcg cccgcgccgc gcaggagggc gcgcatctcc tgaatctttt
     3001 gcaggatgct cagagactcg cccagaaagt cgttgatgct cgtcgtcaat tttttctttt
     3061 cgccgctcgt ctgctcgccc tctgccgcgc cgtcgcccgc gtcggtcgtc ccggcggcgg
     3121 cggcctcttc ggtcaggcgc gagacggcac cctcaagctc ctgccggcgc gaggcgatgt
     3181 cggcgtcggc ccgcgcggtg tcctgcgcct gttcgaggcg ggcggccttg gcagcgaggg
     3241 cacgttgcag accttcgagg gcgcggttga gcgcgccgag actgtcggcg ctgcccgccc
     3301 cgctcgcggc gagcgactcg gcctgcgcga tgagctgctg tagcgcttcg tcgtcttcga
     3361 cggcgcccgc tgcttccgcg tcgctttcgc cctgcgtctc cgcggtcgca ggcgccgcgc
     3421 cctcatccgc gcttgacggc gcgggcgctt cggggtcgct ctcgcccgac ggcggcggcg
     3481 cttgggcgcc gtccgctgca tcgcctgacg gcgagacgac ggccctgtaa acctccgcgg
     3541 cgagcttttc gaccgcggcg ttgagggccg cgcccgcatc cgcgcccccg ccttcggccc
     3601 gcacgccgcg caattcgccg tcctcgaccg acaccacttc ggcgtaaacc tgcgcgacca
     3661 cggctgcgcc cgcgccgcta gtctccacgg cgagcacgcc cccgcgcacg acgaacttca
     3721 gcccacgctg tttaccgaac gcggcgagct gttcgacgcc gagcgtgccc gccggggccg
     3781 cgcccggctc ggcgccgacg gcacgcggga gcaagtcctt gtgcgtggcg gcgagctttt
     3841 gctggagcgt gcgggcgagc tggccgacaa gaccgggcgc gacttcggcc ccgctctcgt
     3901 cctgaaaggc gacgacgccg accgtgacga cctgtccctg cgccgcgcgc gtcgcccctg
     3961 cgccgccccg gaggcccaag caaacgatga tcagggcgac gccgagaacg accagcgggc
     4021 tgcgtttact gcacttggac gacctcatat ctctccttct cggggcggca cccggcggcc
     4081 ctcacttcgt taaggcgcag ggcgggcgcg aaaactagca gccgccgcgg
//
LOCUS       DBNGOH010000976         6898 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3446611, whole genome shotgun sequence.
ACCESSION   DBNGOH010000976 DBNGOH010000000
VERSION     DBNGOH010000976.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6898)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6898)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6898
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3446611"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3816
                     /locus_tag="AC159P_19030"
     CDS             <1..3816
                     /locus_tag="AC159P_19030"
                     /inference="COORDINATES: protein motif:HMM:NF051698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HIR3697588.1"
                     /translation="VSVAAAITALGQGTTSRVTGIVQDPTGAAVENATVTLRNEATSV
                     TITTQTTSAGAYVFDSVQVGTYTLAVEVPGFKKFAATGNAINVNQPATIDVVLEAGNI
                     DETVTVESAAELVQTSTSGNFGNTVEERPLEALPIVGTRGRNPLNFINFQPGVTPDAN
                     TGGGVHVHGARDRAFNFTLDGIDINETSAGGSNFTPIRPNPDSITQFQVITSNFTADN
                     GRSSGAQVTLVTKSGTNDFHGNIFEFYQTPRFHANEYTNTINTRLVNGQRVPTDKPQF
                     VQHIFGGSLGGPVYLPRFGEGGDRVYDGHDKTFFFVNLQLLRTSQAFFVNRTVYTEQA
                     RAGLFRYSVGRANANANATGNARVIDANGVPLFPNCSGSVTTNCIATFGLVGNPNIPL
                     TPDPTTNGLIALTPLPNNFTIGDGLNTAGFSFIAPQTERQYDFTTKIDHNFNDRHAVF
                     VRYARGEQTTLCDNVNGGLQRFPDSPCFVDTTRSPRNLAVNYRATITPTTVNEFVAGF
                     NRFTFGFLNPDPNAATNPPIILNIPTDPLNREAPLNNARRITTWQFVDNLSHQRGAHT
                     LKFGTNIRLQEHFDDRTSVAGLLTVPTVRLGTGNNAVPLAFGTRALPTGNAGINVTDR
                     GTLESFINDFLGRIGRVEQSFVAADDSAFAPAGTRFNYTADYPEYDFYGQDTWKLTPN
                     LTIDYGLRWEVRFSPRSDNLLLRPATPVRFGETATNQIRFEEGKLYDDSWKQFAPTVG
                     FAWDPFGSGKTSVRGNYRLAWDRTNTFVFSSFIFQSAPGLTRAITLLASSPTFGGANN
                     ALLRNGIPTLSANDTTPLAFRTPPAFSVNTLTVVDPELSYPRTHQYGFSFQREVGWSS
                     VLEVNYIGRQGRKLFGGYDANQVDITNNGFLAAFRQLQDPATRAGVLTNPNFLINQLM
                     AGDSRLSAGQTGAQFLLGQSLGTGLVSVPLANGQTAANIVDAGSVAQAAFLIAQGTRS
                     GNSTTPNFQLNGFSPFFFQSYPQFTGAVNVIDNNDRSRYNALEVQLSRRFSRGLGFQV
                     SYTLAKSEDTRSFDPAFAIANRGFVQSASNSPYDIRNREANYARSDFDRRHALQGYFT
                     TELPFGRGRRWLSDANPVVNHLLGGFELAGIVRWYSGRPFTVYSGINSVSQSVQSPAS
                     CNGCTPDMGNIVLDGGRNFIFSPDQRATFFAPAAGELGNTGRNFFTGPKLFQLDLTLG
                     KRIRFDESRNLELRLEAQNATNTPSFGLPTGTPTDVNLNIASGNFGLVGGNTVSGSRK
                     VQIAAKFNF"
     gene            4183..5826
                     /locus_tag="AC159P_19035"
     CDS             4183..5826
                     /locus_tag="AC159P_19035"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014101205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl-D-amino-acid deacylase family protein"
                     /protein_id="HIR3697589.1"
                     /translation="MKSSRTTSRALPRALLLSALAALLAAGAGRMHRAQETAAPPPAP
                     AYDLVITNARVVDGAGNPWFRADVAVREGRIARVGRIAASEGRSSIDARNRILAPGFI
                     DVHTHVEGVYNLPAAENFVRMGVTTLVTGNCGGSVVDVGRFLARASETPLAVNIATLV
                     GHGSVRESVMGEVDRAPTPEELEKMKALVGQAMKDGAVGLSTGLIYVPGTYAKTAEVV
                     ELARVVAQHGGVYATHMRSEGEHVREAIRESIEIGEKAGLPVEISHFKISMKKLWGRS
                     DATLGLVREARARGLSVTVDQYAYTASSTSLDSRFPSWVLEGGREEGKKRLADPAQRA
                     RVVRELKASLKKSGFKDFSYAVVGSFRAKPEYNGKSIAEITKLARGKKDIDSQIAQLV
                     EMYEAGGASMVYHSMGEEDVRRIMREPFTMIASDSGVRRFGEGVPHPRGYGNNARVLG
                     LYVRELGVISLEDAVRKMTSLPAQTFGLRDRGLVREGLAADLVIFDEKLVGDRATFEQ
                     PHQYAIGLDFVFVNGQAVVAEGQPTPARPGAALRHAGRD"
     gene            5947..>6898
                     /locus_tag="AC159P_19040"
     CDS             5947..>6898
                     /locus_tag="AC159P_19040"
                     /inference="COORDINATES: protein motif:HMM:NF025006.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HIR3697590.1"
                     /translation="MQDAAERLPRALVVIAGVVIALCALAPGAAAQTVTGTMQGTVKD
                     TNDAVVTGATVTIRNVETGQERTVTTNSEGYFVAPFLPIGRYEATATQSGFGPVKREG
                     LEVTLNQTAVADFTLNPTVAEEVTVVTDEAPINVTNQEVKGSLNTQQIQDKPTLNQGS
                     FLSLAETFSGYQENPVSGQNNPTASSGSSINFNGTGTRGATFQINGVNNDDSSENQNR
                     QGVSLATIKEFQVITNNFTAEFGRGYGAVVLVQTLSGTNRIRGSAYWFNQNSALNATN
                     NVFNPTAVKPINRRNQFGMTGGFPVIKNKLFGFVSWDHVED"
BASE COUNT         1233 a         2605 c         2063 g          997 t
ORIGIN      
        1 gtctcggtgg cggcggccat caccgccctc gggcagggca ccacttcgcg cgtgaccggc
       61 atcgtgcagg acccgacggg cgcggcggtc gagaacgcga ccgtcacgct ccgcaacgag
      121 gcgacgagcg tcaccatcac gacgcagacg acctccgccg gcgcctacgt cttcgactcc
      181 gtgcaggtcg gcacctacac gctcgcggtc gaggtgcccg gcttcaagaa gttcgccgcg
      241 acgggcaacg ccatcaacgt caaccagccc gcgaccatcg acgtggtgct cgaagccggc
      301 aacatcgacg agacggtgac ggtcgagtcc gccgccgaac tcgtccagac gagcacctcc
      361 ggcaacttcg gcaacacggt cgaggagcgc ccgctcgaag cgctccccat cgtcggcacg
      421 cgcgggcgca acccgctcaa cttcatcaac tttcagcccg gcgtgacgcc cgacgccaac
      481 accggcggcg gcgtccacgt ccacggcgcc cgcgaccgcg ccttcaactt cacgctcgac
      541 ggcatcgaca tcaacgagac cagcgccggc ggctcgaact tcacgcccat ccgcccgaac
      601 cccgactcga tcacgcagtt ccaggtcatc accagcaact tcaccgccga caacggtcgc
      661 tcctcgggcg cgcaggtcac gctcgtcacc aagtcgggca ccaacgattt ccacggcaac
      721 atcttcgagt tctaccagac gccgcgcttc cacgccaacg agtacacgaa cacgatcaac
      781 acgcgcctcg tcaacggcca gcgcgtgccg accgacaagc cgcagttcgt ccagcacatc
      841 ttcggcggca gcctcggcgg ccccgtctac ctcccgcgct tcggcgaggg cggcgaccgc
      901 gtctacgacg gccacgacaa gaccttcttc ttcgtcaacc tccaactgct gcgcacgagc
      961 caggcgttct tcgtcaaccg cacggtctac accgagcagg cgcgcgccgg cctcttccgc
     1021 tactcggtgg gccgcgccaa cgccaacgcc aacgcgaccg gcaacgcgcg cgtcatcgac
     1081 gccaacggcg tcccgctctt cccgaactgc tcgggcagcg tcacgaccaa ctgcatcgcc
     1141 accttcggcc tcgtcggcaa cccgaacatc ccgctcacgc ccgacccgac cacgaacggc
     1201 ctcatcgcgc tgacgccgct gccgaacaac ttcaccatcg gcgacggcct caacacggcg
     1261 ggcttcagct tcatcgcgcc gcagaccgag cggcagtacg acttcacgac caagatcgac
     1321 cacaacttca acgaccgcca cgccgtcttc gtccgctacg cgcgcggcga gcagaccacg
     1381 ctctgcgaca acgtcaacgg cggcctccag cgcttccccg actcgccctg cttcgtcgat
     1441 accacgcgct cgccgcgcaa cctcgccgtc aactaccgcg cgacgatcac gccgacgacc
     1501 gtcaacgagt tcgtcgccgg cttcaaccgc ttcaccttcg gtttcctcaa ccccgacccg
     1561 aacgcggcca ccaacccgcc gatcatcctc aacatcccga ccgacccgct gaaccgcgag
     1621 gcaccgctca acaacgcgcg ccgcatcacg acctggcagt tcgtcgataa cctctcgcac
     1681 cagcgcggcg cgcacacgct gaagttcggc acgaacatcc gtttgcagga acacttcgac
     1741 gaccgcacct cggtcgccgg cctgctgacg gtgccgaccg tccgcctggg cacgggcaac
     1801 aacgcggtcc cgctcgcgtt cggcacgcgc gccctgccga ccggcaacgc cggcatcaac
     1861 gtcaccgacc gcgggacgct cgaaagcttc atcaacgact tcctgggccg catcggtcgc
     1921 gtcgagcagt cgttcgtcgc cgcggacgac tcggccttcg ccccggcggg cacccgcttc
     1981 aactacacgg cggactaccc cgagtacgac ttctacgggc aggacacgtg gaagctgacg
     2041 ccgaacctga ccatcgacta cggcctgcgc tgggaggtgc ggttcagccc gcgctccgac
     2101 aacctgctgc tgcgccccgc gacgccggtg cgcttcggcg agacggcgac caaccagatc
     2161 aggttcgagg agggcaagct ctacgacgac tcgtggaagc agttcgcgcc gacggtcggc
     2221 ttcgcgtggg acccgttcgg cagcggcaag acctcggtgc gcggcaacta ccgcctcgcg
     2281 tgggatcgca ccaacacgtt cgtcttctcc tcgttcatct tccagagcgc gcccggtctg
     2341 acgcgcgcca tcacgctgct cgccagcagc cccaccttcg gcggggccaa caacgcgctg
     2401 ctgcgcaacg gcatccccac gctctcggcc aacgacacga cgccgctcgc cttccgcacg
     2461 cccccggcct tctcggtgaa cacgctgacg gtcgttgacc ccgaactcag ctacccgcgc
     2521 acgcaccagt acggcttcag cttccagcgc gaggtcggct ggagttccgt cctcgaagtc
     2581 aactacatcg gtcggcaggg gcgcaagctc ttcggcggct acgacgccaa tcaggtggac
     2641 atcaccaaca acggcttcct cgccgccttc cggcagcttc aagacccggc gacgagggcg
     2701 ggcgttctga ccaacccgaa tttcctcatc aaccagttga tggccggcga ctcgcgcctc
     2761 agcgcggggc agacgggcgc gcagttcctc ctcgggcagt cgctcgggac ggggctggtc
     2821 agcgtccccc tcgccaacgg gcagaccgcc gcgaacatcg tcgatgcggg cagcgtcgcg
     2881 caggcggcct tcctcatcgc gcagggcacg cgctccggca acagcacgac cccgaacttc
     2941 cagctcaacg gcttcagccc cttcttcttc cagtcgtacc cgcagttcac gggcgccgtc
     3001 aacgtcatcg acaacaacga ccgctcgcgc tacaacgcgc tggaagtcca gctcagccgc
     3061 cgcttctcgc gcggcctcgg cttccaggtc agctacacgc tcgccaagtc ggaggacacc
     3121 cgctcgttcg acccggcctt cgccatcgcc aaccgcggct tcgtgcagtc ggcttccaac
     3181 tcgccttacg acatccgcaa ccgcgaggcc aactacgccc gctccgactt tgaccggcgc
     3241 cacgccctcc agggctactt cacgacggag ctgcccttcg ggcgcgggcg gcggtggctg
     3301 agcgacgcca accccgtcgt caaccacctc ctcgggggct tcgagctggc cggcatcgtg
     3361 cgctggtatt cgggccgccc gttcaccgtc tactccggca tcaactccgt ctcgcagtcg
     3421 gtgcagtccc ctgcgagctg caacggctgc acgcccgaca tggggaacat cgtcctcgac
     3481 ggcggcagaa acttcatctt ctcccccgat cagcgcgcca ccttcttcgc cccggcggcg
     3541 ggcgaattgg ggaacacggg ccgcaacttc ttcaccggcc ccaagctctt ccagctcgac
     3601 ctgacgctcg gcaagcgcat ccgcttcgac gagagccgca acctcgaact ccgcctcgaa
     3661 gcgcagaacg cgacgaatac gccttcgttc ggcctgccga ccggcacgcc gacggacgtc
     3721 aacctcaaca tcgcgagcgg caacttcgga ctggtcggcg gcaacaccgt cagtggctcg
     3781 cgcaaggtgc agatcgccgc caagttcaac ttctgacccg cacgcctcga ccgaaggcga
     3841 cgcgcttcga ccactctccg ggccgcggcc agatggctcg gcccggagat tttttcgagt
     3901 ccgccgggca ggtttggcgg gggcgcggcc cctgcgcggg ccacaagttt tgcttatccg
     3961 gatgataaaa tcttttagct ttgattattg caggggcgtt ggctaaaata cgcccacgaa
     4021 cgcagcacaa gggtttcagg ccgcctctgt tcttcgcctg caagagggtt tcaagtcgga
     4081 aagcgtgcac cgtccccggc gatccgccgc cctacttgcc cgccggatcg cgtgcgcggc
     4141 acacggcaag cgcggcggcc ctcgtccacc actcttgaac atatgaaatc atcccgcacg
     4201 acgagtcgcg cgcttccgcg cgctctcctg ctgtcggccc tcgccgcctt gctcgcggcg
     4261 ggcgccgggc gcatgcatcg cgcgcaggag acggcggcgc cgccgcccgc gcctgcgtac
     4321 gacctcgtca tcacgaacgc gcgcgtcgtg gacggcgcgg gcaacccctg gttccgcgcc
     4381 gacgtggccg tccgtgaagg gcgcatcgcg cgcgtcgggc gcatcgccgc gagcgagggc
     4441 cgaagcagca tcgacgcgcg gaacagaatc ctcgcgcccg gcttcatcga cgtgcacacg
     4501 cacgtcgagg gcgtctacaa cctgccggcg gcggagaact tcgtccgcat gggcgtgacg
     4561 acgctcgtca cgggcaactg cggcgggtcg gtggtggacg tggggcgctt cctcgcgcgc
     4621 gcctcggaga cgccgctcgc cgtcaacatc gccacgctcg tcggccacgg ctcggtgcgc
     4681 gagagcgtga tgggcgaggt ggatcgcgca ccgacgcccg aagagctgga gaagatgaag
     4741 gcgctcgtcg ggcaggcgat gaaggacggc gcggtcggcc tctcgaccgg cctcatctac
     4801 gtgcccggca cctacgcgaa gaccgccgag gtcgtggagc tggcccgcgt cgtcgcgcag
     4861 cacggcggcg tctacgccac gcacatgcga agcgagggcg agcacgtccg cgaggccatc
     4921 cgcgagagca tcgagatcgg cgagaaggcg ggcctgcccg tcgaaatctc gcacttcaag
     4981 atcagcatga aaaagctgtg gggccggagc gacgcgacgc tcggcctcgt gcgcgaggcg
     5041 cgcgcgcgcg gactctccgt gacggtcgat cagtacgcct acacggccag ctccacgtcg
     5101 ctcgactcgc gtttcccgtc gtgggtgctc gaaggcgggc gcgaggaagg gaagaagcgg
     5161 ctcgccgacc cggctcagcg cgcgcgcgtc gtccgcgagc tgaaagcctc tctgaagaag
     5221 agcggcttca aggacttctc gtacgcggtg gtcggcagct tccgcgccaa gcccgagtac
     5281 aacggcaagt ccatcgccga gatcacgaag ctcgcgcgcg gcaagaagga tatcgacagc
     5341 cagatcgcgc agctcgtcga gatgtacgag gcgggcggcg cctcgatggt ctaccacagc
     5401 atgggcgagg aggacgtgcg ccgcatcatg cgcgagccgt tcacgatgat cgcctccgac
     5461 agcggcgtgc gccgcttcgg cgagggcgtc ccgcacccgc gcggctacgg caacaacgcg
     5521 cgcgtcctcg gcctctacgt ccgcgagctg ggcgtcatct cgctcgaaga cgccgtgcgc
     5581 aagatgacct cgctcccggc gcagaccttc ggcctgcgcg accgcgggct ggtgcgcgag
     5641 gggctggccg ccgacctcgt catcttcgac gagaagctcg tcggcgaccg cgccaccttc
     5701 gagcagccgc accagtacgc catcggcctc gacttcgtct tcgtcaacgg ccaggccgta
     5761 gtcgccgaag gccagccgac gcccgcgcgc cccggcgccg cgctccgcca cgcgggccgc
     5821 gactgaacac gacccgagtc ccgcgcagtg acggcccgtc atccttcgcg cgggccgcag
     5881 ttctccacgg tcgatccacg ggggcagatt tcagaagaat cagattgaga gaggaaggaa
     5941 acctttatgc aggacgcagc cgaacgtttg cccagggctt tggtcgtcat cgccggcgtg
     6001 gtcatcgccc tctgcgcgct cgcgcccggc gccgccgcgc agaccgtgac cggcacgatg
     6061 caaggcaccg tgaaggacac caacgacgcc gtcgtcacgg gcgcgaccgt caccatccgc
     6121 aacgtcgaga cggggcagga gcgcaccgtc acgaccaaca gcgagggcta cttcgtcgcc
     6181 ccgttcctcc ccatcggcag gtacgaagcg accgccacgc agtcgggctt cggcccggtc
     6241 aagcgcgagg ggctggaggt cacgctgaac cagaccgcgg tggccgactt cacgctcaac
     6301 ccgacggtgg ccgaagaggt cacggtcgtc accgacgagg cgcccatcaa cgtcaccaac
     6361 caggaggtca aggggtcgct caacacgcag cagattcagg acaagccgac cttgaatcag
     6421 ggcagcttcc tctcgctcgc cgagaccttc agcggctacc aggagaaccc cgtctccggg
     6481 cagaacaacc cgaccgcctc ctccggctcg tccatcaact tcaacggcac gggcacgcgc
     6541 ggcgccacct tccagatcaa cggcgtcaac aacgacgact ccagcgagaa ccagaaccgg
     6601 cagggcgtct ctttggcgac gatcaaagag ttccaggtca tcaccaacaa cttcaccgcc
     6661 gagttcgggc gcggctacgg cgcggtcgtc ctcgtccaga cgctctcggg cacgaaccgg
     6721 atccgcggca gcgcctactg gttcaaccag aacagcgcct tgaacgcgac caacaacgtc
     6781 ttcaacccca ccgccgtcaa gcccatcaac cggcgcaacc agttcggcat gaccggcggc
     6841 ttccccgtca tcaaaaataa actgttcggc ttcgtcagtt gggatcacgt cgaggaca
//
LOCUS       DBNGOH010000977         2054 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_5096664, whole genome shotgun sequence.
ACCESSION   DBNGOH010000977 DBNGOH010000000
VERSION     DBNGOH010000977.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2054)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2054)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2054
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_5096664"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1365
                     /locus_tag="AC159P_19045"
     CDS             <1..1365
                     /locus_tag="AC159P_19045"
                     /EC_number="2.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014798.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016744 - transketolase or transaldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HIR3697591.1"
                     /translation="EDGNDLAALEAAIDEARSVTDRPSLISVKTVIGYGMPTQGTRKA
                     HSDAPGEEAVKETKRALGWPEDKEFYIPDEALAHFREAIERGEKLETEWNALVGRYEQ
                     EHGELAAAWRTTMSGELPAEWERHLPSFENAKAVATRAASGECINALAPHMPMLVGGS
                     ADLGPSNNTDIKDGGSFQHGSYAGRILHFGVREHAMGATLTGMSLNGGLIPYGGTFLT
                     FSDYMKPAIRLAALSEVQVVYVFTHDSVGLGEDGPTHQPIEHLAALRSIPHLHVIRPA
                     DVHEVREAWRAAILRRHAPTALILTRQKVPLIDRNGAYASAEGARRGAYVLAEATDKE
                     GKRAAPRLVILATGSEVHLALEARTALQQEDVPTRVVSMPCWEAFDEQDAEYRESVIP
                     SAVAARLAVEAATPFGWHRYVGERGDVIGLDRFGASAPGDVALKNLGFNVEHVLERAR
                     ALLK"
     gene            complement(1602..>2054)
                     /locus_tag="AC159P_19050"
     CDS             complement(1602..>2054)
                     /locus_tag="AC159P_19050"
                     /inference="COORDINATES: protein motif:HMM:NF039905.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol/lipid kinase family protein"
                     /protein_id="HIR3697592.1"
                     /translation="VAAQGQSLRLLPGEARFALAAMRPIAAWRNRRVRVALGGVGWEE
                     LRTNFVVVCNGAYAGGGMNFAPAAQTDDGLLDVLTVGDISRAGVVREMSRIHRGGHVA
                     NPKVRIRRAARVCVESLEDELMIEADGDFRGHTPAEFVVMPSALRVVW"
BASE COUNT          346 a          769 c          647 g          292 t
ORIGIN      
        1 gaggacggca acgatctggc cgcgctcgaa gccgcgatcg acgaagcccg ctccgtgacc
       61 gaccgcccct cgctcatctc cgtcaagacc gtcatcggct acggcatgcc cacgcagggc
      121 acgcgcaagg cgcactcgga cgcgcccggc gaagaggccg tcaaagagac caagcgcgcg
      181 ctcggctggc ccgaagacaa agagttctac atcccggacg aggcgctcgc gcacttccgt
      241 gaagcaatcg agcgcggcga gaaactggaa accgagtgga acgcgctcgt cggtcgctac
      301 gagcaggagc acggcgaact tgccgccgcg tggcgcacga cgatgagcgg cgagctgccc
      361 gccgaatggg aacgacacct cccctcgttc gagaacgcga aggcggtggc gacgcgcgcc
      421 gcgagcggcg aatgtatcaa cgcgctcgcg ccgcacatgc cgatgctcgt cggcggcagt
      481 gccgacctcg gcccctcgaa caacacggac atcaaggacg gcggcagctt tcagcacggc
      541 tcttacgccg gtcgcatcct ccacttcggc gtgcgcgagc acgcgatggg ggcgacgctg
      601 acgggcatga gcctcaacgg cggcctcatc ccctacggcg gcacgttcct caccttctcc
      661 gactacatga agcccgccat ccgcctcgcg gccctgtccg aggtgcaggt cgtctacgtc
      721 ttcacgcacg actcggtcgg cctcggcgaa gacggcccca cgcaccagcc catcgaacat
      781 ctcgcggcgc tgcgctcgat cccgcacctg cacgtcatcc gcccggcgga cgtgcacgaa
      841 gtccgcgagg cttggcgcgc ggcgatcctg cgccgccacg cgccgaccgc gctcatcctc
      901 acgcgccaga aggtgccgct catcgaccgc aacggcgcat acgcttcagc cgagggcgcg
      961 cgccgcggcg cctacgtcct cgccgaagcg acggacaagg aaggcaaacg agcggcgccg
     1021 cgactcgtca tcctcgccac cggctccgaa gttcacctcg cgctcgaagc gcgcaccgcg
     1081 ttgcagcagg aagacgtgcc gacgcgcgtc gtctcgatgc cctgctggga ggcgttcgac
     1141 gagcaggacg cggagtaccg cgagagcgtc atcccttctg cggtcgccgc gcggctcgcg
     1201 gtcgaagccg ccacgccgtt cggctggcac cgctatgtcg gcgagcgcgg cgacgtgatc
     1261 ggcctcgacc gcttcggcgc ctccgccccc ggcgacgttg cgctcaagaa cctcggcttc
     1321 aacgtcgagc acgtcctcga acgcgctcgc gccctgttga aataaggggc cgcatctttt
     1381 ttgtcggagg ttcaccgcgg atgggcgggc acccctggtt ctagccggtt catttcaacg
     1441 cagagtgcgc ggaggagcgc agagggcgca gagaaaacca atcgtcttct ctgcgccctc
     1501 tgcgtgtcct ctgcgttctc tgcgttgaat cctgcttccc ccaaactcag ccgccaactc
     1561 tgccgagacg agtcggagtc caagcgcccg ccccacgtcc ctcaccacac gacgcgcagc
     1621 gccgacggca tcacgacgaa ctcggcgggc gtatgcccgc gaaagtctcc gtcggcctcg
     1681 atcatcaatt cgtcttcgag ggattcgacg cagacccgcg ctgcgcgcct gatccgcacc
     1741 ttcgggttgg cgacgtggcc gccgcggtgg atgcgcgaca tctcgcgcac gacgcccgcg
     1801 cgcgagatgt cgccgacggt gaggacatcg agcaggccgt cgtccgtttg cgccgccggc
     1861 gcgaagttca tcccgccgcc cgcgtaggcg ccgttgcaca cgacgacgaa gttcgtccgc
     1921 agctcttccc acccgacccc tccgagcgcc acgcgcacgc gccggttgcg ccacgcggca
     1981 atcggtctca tggcggcgag cgcgaaccgc gcctcgcccg gcaacagcct gagcgactgc
     2041 ccttgcgccg cgac
//
LOCUS       DBNGOH010000978         1530 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1799397, whole genome shotgun sequence.
ACCESSION   DBNGOH010000978 DBNGOH010000000
VERSION     DBNGOH010000978.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1530)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1530)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1530
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1799397"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..551
                     /locus_tag="AC159P_19055"
     CDS             <1..551
                     /locus_tag="AC159P_19055"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016865489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HIR3697593.1"
                     /translation="FLALDNGFKREDSYKDIGALLDWLPTQPRLDASRVMVTGGSYGG
                     HMAFAIATLYSDKIRASLPVVGISNLVSFLERTESYRRDLRRAEYGDERDPRMRAFMT
                     HIAPLTNASKITKPIFIVQGGNDPRVPLNEAEQMVKTVRQNNTPVWYLMAKDEGHGFN
                     KKKNQDFQFYATIMFVREYLLK"
     gene            820..>1530
                     /locus_tag="AC159P_19060"
     CDS             820..>1530
                     /locus_tag="AC159P_19060"
                     /inference="COORDINATES: protein motif:HMM:NF052711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697594.1"
                     /translation="MIIRPFIISTARALLVACCLLVSLGALACKSDYPASGQQKGPGG
                     GEGKGGAGGGEARQVKVARVAEVPVGSSVTVTGTLAAKDEATLSVKVPGRLSMIAVDL
                     GSGVRRGQVVAQVERQDYAIRVQQAEAALQQARARLGLALDGSSDRVDVEETGAVRQA
                     RAVLDEARVSRQRTVTLVEQGVVSRAEYDAANAAFKVAQSRYQDAIEEIRNRQGMLAQ
                     RRSEVALARQALADTVITA"
BASE COUNT          280 a          498 c          496 g          256 t
ORIGIN      
        1 ccttcctcgc gctcgacaac ggcttcaagc gcgaggacag ctacaaggac atcggcgcgc
       61 tcctggactg gctgccgacg cagccgcgcc tcgacgcctc gcgcgtgatg gtcacgggcg
      121 gctcctacgg cggccacatg gcgttcgcca tcgccacgct ctacagcgac aagatacggg
      181 cgtcgctccc cgtggtcggc atctcgaacc tcgtctcgtt cctcgaacgc accgaatcct
      241 accgccgcga tctgcgccgc gccgagtacg gcgacgagcg cgaccccagg atgcgcgcct
      301 tcatgacgca catcgcgccc ctgaccaacg cctccaaaat caccaagccg atcttcatcg
      361 tgcagggcgg caacgacccg cgcgtgccct tgaacgaggc cgagcagatg gtcaagaccg
      421 tccgccagaa caacacgccc gtctggtacc tgatggcgaa ggacgagggc cacggcttca
      481 ataaaaagaa gaaccaggat ttccagttct acgccaccat catgttcgtc cgcgagtacc
      541 tgctgaaata actgaagggg agacagggag aaggggagaa aagaagagca agcgagtcgc
      601 ctgtcttccc tatcttccct gtctccccgt ctccctgtct ccctctctcc ctgtctccct
      661 gtctcccctt ctctcttttc cctcgcatct ttttacaaaa gctttaccaa atccgcactt
      721 ttgctgaaat gggaagggct aaattattcg taaccggcag gctaaacttt tcgtctatta
      781 aacttgaata gcaatctcgg cggggatgtt cagcgaaaag tgattattag accgtttatt
      841 atctccacgg cgcgcgcgct gctggtcgcg tgctgcctgt tggtgtctct gggcgctctc
      901 gcgtgcaaga gcgactaccc tgcgtcgggc cagcagaagg ggccgggcgg cggcgagggg
      961 aagggcggcg cgggcggcgg cgaggcgcgg caggtgaagg tcgcgcgcgt ggccgaagtg
     1021 ccggtcgggt cgagcgtcac ggtgacgggc acgctcgcgg cgaaggacga ggcgacgctg
     1081 agcgtgaagg tgccgggccg cctgagcatg atcgcggtcg atctcggcag cggcgtgcgg
     1141 cgcgggcagg tcgtggcgca ggtcgagcgg caggactacg cgatccgcgt ccagcaggcc
     1201 gaggcggcgc tgcaacaggc gcgcgcgcgt ctcggcctcg cgctcgacgg ttcgagcgac
     1261 cgcgttgacg tggaagagac cggcgccgtc aggcaggcgc gggccgtgct ggacgaggcg
     1321 cgcgtgtcgc gtcagcgcac cgtcacgctc gtcgagcagg gggtggtctc gcgcgccgag
     1381 tacgacgccg ccaacgccgc cttcaaggtc gcacagagcc gctaccagga cgcgatcgaa
     1441 gagatacgca accgtcaggg catgctcgca cagcggcgtt ccgaagttgc gctcgcgcgg
     1501 caggcgctcg ccgacacggt cattacggcg
//
LOCUS       DBNGOH010000979         7510 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_331881, whole genome shotgun sequence.
ACCESSION   DBNGOH010000979 DBNGOH010000000
VERSION     DBNGOH010000979.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7510)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7510)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7510
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_331881"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            257..1180
                     /locus_tag="AC159P_19065"
     CDS             257..1180
                     /locus_tag="AC159P_19065"
                     /inference="COORDINATES: protein motif:HMM:NF015431.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR3697595.1"
                     /translation="MEMSQLEFFLKVIEEGSFSKAADSVGRTQPAVSIAIRRLEEEVG
                     APLFDRSQKTPTLTEAGEIIHDYAQRILTLREHARASVSELRTLQRGRVRVGANESTS
                     LYLLPQIILAYRESHPQVKVEIYRQVSERLPREVLERNVDFAVLAFEPVDRDLESFCV
                     LKDELALILNPEHPLARRPSLTVAELGSESFLAHNVRTASRNKVIEVFAEHHTPLNIT
                     LELATVETIKRFVQLKVGLAFVPRMCVREELERGTLATVPVEGLNYHRSLWVTHRRNM
                     TFSHAAAAFLKVLRQHAKLQQAGNDKEREKG"
     gene            1397..2629
                     /locus_tag="AC159P_19070"
     CDS             1397..2629
                     /locus_tag="AC159P_19070"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: protein motif:HMM:NF002086.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HIR3697596.1"
                     /translation="MISANRIAVFDTTLRDGEQSPGCSMNLDEKVRLAHQLEALGVDI
                     IEAGFPIASEGDFAAVQAVAAECRGVTVAGLARTSEQDIVRAGSALRRAARPRIHTFV
                     ATSDIHLEHKLKKTRAEVLRMTRDAVRLARTLAEEVEFSAEDATRSDVDYLCEVFAAA
                     VDEGARVLNVPDTVGYTTPWEFARLVETVRARVVKEREDVIISVHCHNDLGLAVANSL
                     AAINAGARQVECTVNGIGERAGNAALEEVVMAMRVRADQLPFETGVVTERIYPTSQLL
                     SEIINCAVQPNKAVVGRNAFAHEAGIHQHGVINNPLCYEIMTPDSVGVPANSLVLGKH
                     SGRHALALRYAELGHALSAAELDAVYRRFTELADRKKRVYDQDLLALLPAVAQTVGGR
                     HGVEEVFAAREVATSASY"
     gene            2762..3874
                     /gene="leuB"
                     /locus_tag="AC159P_19075"
     CDS             2762..3874
                     /gene="leuB"
                     /locus_tag="AC159P_19075"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020712035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HIR3697597.1"
                     /translation="MKLKITVLPGDGIGPEVTREAVRVLRAVASSYEHEFEFDERAVG
                     GAAIKESGSPLPAATLDACLSGDAVLLGAVGAPEFDALTPDKRPEAGLLALRRALGAY
                     ANLRPAVLHAAIADASPLRAEVVEGTDILIVRELLGGLYFGEPRGTSGEAGSRVAFNT
                     MSYSEEEVERVARVAFEAALGRSGRVTSVDKANVLETSQLWRAVVSRVALDYPAVALD
                     HLYVDACAMHLVTNPRRFDVILTENLFGDILSDEAAVITGSLGLLASASIGGPVGLYE
                     PVHGSAPDIAGRDTANPLGAIASAALLLRYSARLEQEAEDVEAAIRSVLDAGYRTPDI
                     DRGTGGKYLARTSEIGQLVAEAVAETADMRHAYHAV"
     gene            3977..5380
                     /gene="leuC"
                     /locus_tag="AC159P_19080"
     CDS             3977..5380
                     /gene="leuC"
                     /locus_tag="AC159P_19080"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014264459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HIR3697598.1"
                     /translation="MSTLFDKLWDDHVVCEPAGEPALLYIDLHLVHEVTSPQAFEGLR
                     AAGRKLRRSDLTVATVDHNVPTTDRSLPIADPIAARQIETLRENCREFGVALYDIDSA
                     EQGIVHVVGPELGATQPGMTIVCGDSHTSTHGAFGALAFGIGTSEVEHVLATQTLPQS
                     KPRTMLVRTSGALPAGVTAKDLALGIIGEIGTDGATGYVIEYAGAAVRELSMEGRMTL
                     CNMSIESGGRAGMVAPDEVTFAYLESRPFAPQGAAWDEALRYWQTLKSDEDARFDRVV
                     EIDASSLAPFVTWGTSPGQVVAVTGRVPDPADAASEADRRAAERALDYMNLKPLTRVE
                     EIGIDRVFIGSCTNARIEDLRAAARVIRGYKADPKVSAMVVPGSQAVKRAAEAEGLDA
                     VFRDAGFDWREPGCSMCLGMNPDPLLPGERCASTSNRNFEGRQGRGGRTHLVSPAMAA
                     AAAITGHFTDVRSWEYK"
     gene            5465..5668
                     /locus_tag="AC159P_19085"
     CDS             5465..5668
                     /locus_tag="AC159P_19085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697599.1"
                     /translation="MAEDHQKIKQVDVKTMEAALARTFHELTGAFYKCDIAGITYGDG
                     RINEGVEITMTLHEDTFADDSGS"
     gene            5718..6341
                     /gene="leuD"
                     /locus_tag="AC159P_19090"
     CDS             5718..6341
                     /gene="leuD"
                     /locus_tag="AC159P_19090"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016484035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HIR3697600.1"
                     /translation="MIPFRTHNGLVVPLDQPNVDTDQIIPKQFLKRIERTGFGEFLFY
                     DWRYGADGELDRSFALNEPRYQGASILVAGRNFGCGSSREHAPWALADYGFRAVIAPS
                     FADIFANNCTKNGLLPVVLEEEEVAEIVRRARGGEGYRLTVDLERRVVEDGEGFRASF
                     RIDDFRRYCLLEGLDDIGLTLRRDAEIAAYESRRPAWRAAVTSFNGG"
     gene            6410..>7510
                     /locus_tag="AC159P_19095"
     CDS             6410..>7510
                     /locus_tag="AC159P_19095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002632214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HIR3697601.1"
                     /translation="MTQKQVESRKLATLLEVSQTLAGAHDLRSALSRVLETLASHHSM
                     PRGLVLLLERETDELRLAAAHGLAENGGRRAASYRVGEGITGRVAESGKAIIVPQISR
                     EPLFLDRLGERRKRHRDEELSFVCVPVLLGRKAVGVLGVDMRYKPERDFDRTTKFLAI
                     IASLIAQAIKVEHLIESDKQKLLDENIHLKQELRERYDFGHIIGNSGPLRQVYEQVTQ
                     VARTNTTVLIRGESGTGKELIAHAIHYNSLRSGKPFVKVSCAALPETLIESELFGYEK
                     GAFTGAQGRKKGRFELADGGTLFLDEIGELNLSTQIKLLRALQQREFERLGGTETIKV
                     NVRLIAATNKVLETAIADGTFREDLYYRLNVFS"
BASE COUNT         1330 a         2572 c         2418 g         1190 t
ORIGIN      
        1 cccgtctcgc ttcagcctcg ggccactgtc atgaagtccg gtcagggact tttcgcacgg
       61 cttctacaat cttgtggcgc ctcccgcggc gcgagacaaa attgtagcgg cggcttcggg
      121 gatgaactaa actccgcccc gagaggccgt cagccgtccg tacttcgccg tcagagagac
      181 cagcccgcac gaacttaacg cacgcacgcg gccaccgttc tcagcagcct tcgtccgaaa
      241 ggtgtcagag atcagcatgg aaatgtcgca gctcgaattc ttcttgaagg tcatcgagga
      301 gggcagcttc tcgaaggccg cggacagcgt cgggcgcacg cagccggcgg tcagcatcgc
      361 catccgccgc ctcgaagagg aggtcggagc gcccttgttc gaccgctcgc agaagacgcc
      421 gacgctcacc gaggcgggcg agatcatcca cgactacgcg caaaggatcc tgaccctgcg
      481 cgagcacgcg cgcgcgtcgg tctcggagct gcgcaccttg cagcgcggtc gcgtccgcgt
      541 cggcgcgaac gaaagcacct cgctctacct gctgccgcag atcatcctcg cctaccgcga
      601 gagccacccg caggtcaagg tcgagattta ccggcaggtc tccgagcgcc tgccgcgcga
      661 ggtgcttgag cgcaacgtgg acttcgccgt gctggccttc gagccggtag accgcgacct
      721 cgaaagcttc tgcgtgctca aggacgagct ggccctgatc ctcaaccccg agcacccgct
      781 cgcgcgccgc ccgtcgctga cggtcgcgga gctgggcagc gaatccttcc tcgcgcacaa
      841 cgtccgcacg gcctcgcgca acaaggtcat cgaggtcttc gccgagcacc acacgccgct
      901 caacatcacc ctggagctgg ccaccgtcga gaccatcaag cgctttgtcc agctcaaggt
      961 cggcctcgcc ttcgtccccc gcatgtgcgt ccgcgaggag ttggaacgcg gcacgctcgc
     1021 caccgtcccc gtcgagggcc tcaactacca ccgctcgctc tgggtcacgc accgccgcaa
     1081 catgaccttc tcgcacgccg ccgccgcctt cctcaaagtc ctccgccagc acgccaaact
     1141 acagcaagcg ggcaacgaca aagagaggga aaagggttaa gacaaagagg gggcaaaagg
     1201 gcgaaaaggc gaaaaggcga aacagggaga aggttgagac tcctgcctct tcctctttct
     1261 ccctcttcgc cctttcccct tttcgccctt tctcccgtct ccccataaac cggtcttatc
     1321 gctttataaa aattataact ttgattttgc cccgcgcatt ctttagcatg cctcgtttga
     1381 agacggaggc taacggatga taagtgctaa tagaatcgcg gttttcgata cgacgctgag
     1441 ggacggcgag cagtcgccgg gctgttcgat gaatctggac gagaaggtgc ggctcgcgca
     1501 ccagctcgaa gcgctcggcg ttgacatcat cgaggccgga ttccccatcg cctccgaggg
     1561 cgatttcgcc gccgttcagg cggtcgccgc cgagtgccgc ggcgtcacgg tcgctggtct
     1621 ggcccgcacc tccgagcagg acatcgtgcg cgcggggagc gctctcaggc gcgcggcccg
     1681 accgcgcatc cacaccttcg tcgccacctc cgacatccac ctcgaacaca agctgaaaaa
     1741 gacgcgggcc gaggtcttgc ggatgacgcg cgacgcggtg cggctcgcgc gcacgctcgc
     1801 cgaagaggtc gagttctccg ccgaggacgc gacgcgcagc gacgtggatt acctctgcga
     1861 ggtcttcgcg gcggcggtcg atgaaggcgc gcgtgtcctg aacgtgcccg acacggtcgg
     1921 ctacacgacg ccgtgggagt tcgcgcgtct cgtcgagacg gtgcgcgcgc gcgtcgtcaa
     1981 agagcgcgag gacgtgatca tcagcgtcca ctgccacaac gacctcggcc tcgcggtggc
     2041 caacagcctc gcggccatca acgcgggcgc acgccaggtc gagtgcaccg tcaacggcat
     2101 cggcgagcgc gccggcaacg ccgcgctcga agaggtcgtg atggcgatgc gcgtgcgcgc
     2161 ggatcaactg ccgttcgaga ccggcgtcgt caccgagcgc atctacccga cgagccaact
     2221 gctctccgag atcatcaact gtgcggtgca gccgaacaag gcggtggtgg gccgcaacgc
     2281 cttcgcgcac gaggcgggca tccaccagca cggcgtcatc aacaacccgc tctgctacga
     2341 gatcatgacg cccgactcgg tcggcgtgcc cgcgaacagc ctcgtcctcg gcaagcactc
     2401 gggccgccac gcgctcgcgc tccgctacgc cgaactcggc cacgcgctct cggcagccga
     2461 gctggacgcg gtctaccgcc gcttcaccga actggccgac cgcaagaagc gcgtctacga
     2521 tcaggacttg ctcgcgctgc tcccggcggt cgcgcaaacc gtcggcggtc gccacggcgt
     2581 cgaggaggtt ttcgcggcgc gcgaggtggc gacgagcgcg agttactgag atgtgaaggg
     2641 gaaaaagggg gaaaggggaa agggcgaaga gggagaagcg atgagaagta cgctgccctt
     2701 agtcgtctac cttttcgcct tttccccttt tccccttttc gcccttgatt gagttaaaga
     2761 gatgaagttg aagatcacag tcttgccggg cgacggcatc ggcccggagg tgacgcgcga
     2821 ggcggtgcgc gtgctgcgcg cggtggcgag cagctacgag cacgagttcg agttcgacga
     2881 aagagcggtc ggcggcgcgg cgatcaaaga gtcaggctcg ccgctccccg cggccacgct
     2941 cgacgcgtgt ctgtccggcg acgcggtgct gctcggcgcc gtcggcgcgc ccgagttcga
     3001 cgcgctcacg ccggacaagc ggcccgaggc gggactgctc gccctgcgcc gcgcgctcgg
     3061 cgcctacgcc aacctgcgcc cggcagtgct gcacgcggca atcgccgacg cttcgccctt
     3121 gcgcgcggag gtggtcgagg gcacggacat tctcatcgtc cgcgagctgc tcggcggcct
     3181 ctatttcggc gagccgcgcg gcacctcggg cgaggcaggc tcgcgcgtcg ccttcaacac
     3241 catgagctat tcggaggaag aggtcgagcg cgtggcgcgc gtcgccttcg aggcggcgct
     3301 cgggcgctcg ggcagggtta cttcggtcga taaagcgaat gtgctcgaaa cgtcgcagct
     3361 ctggcgcgcg gtcgtctcgc gcgtcgcgct cgactaccct gcggtcgcgc tcgaccatct
     3421 ctacgtggac gcctgcgcga tgcacctcgt gacgaacccg cgccgcttcg acgtgatcct
     3481 gacggagaac ctgttcggcg acatcctctc ggacgaggcg gcggtcatca caggctctct
     3541 cggcctgctc gcctcggcga gcatcggcgg ccccgtcgga ctctacgaac ccgtgcacgg
     3601 ttcggcgccc gacatcgccg ggcgcgacac ggcaaacccg ctcggcgcca tcgcctccgc
     3661 cgcgctgctc ctgcgctact cggcgcggct cgaacaggaa gccgaggacg tggaggcggc
     3721 catccgctcc gtacttgacg cgggctaccg cacgcccgat attgaccgcg gcaccggcgg
     3781 caagtacctt gcgcgcacct ccgagatcgg ccagctcgtc gcggaggccg tcgccgagac
     3841 cgccgacatg cggcacgctt atcacgcagt ttgaaaagcg ggtgttggat ttgggtgtta
     3901 ggttttaggt tctgaatttc cgactctgag actgaccttc tccacacgaa tacccaacac
     3961 ccaacacctt ttttttatgt ccactctttt cgacaagctc tgggacgatc acgtggtgtg
     4021 cgagccggcg ggcgagccgg cgctgctgta catcgacctg catctggttc acgaagtgac
     4081 ttcgccgcag gcgttcgagg gcctgcgcgc ggcagggcgc aagctgcgcc gttccgatct
     4141 gaccgtggcg acggttgacc acaacgtgcc gacgaccgac cggagcctgc ccatcgccga
     4201 ccccatcgcc gcccggcaga tcgagacgct gcgcgagaac tgccgcgagt tcggcgtcgc
     4261 gctctacgac atcgactccg ccgagcaggg catcgtccac gtcgtcgggc cggagctggg
     4321 cgcgacgcag ccgggcatga cgattgtgtg cggcgacagc cacacgagca cgcacggcgc
     4381 gttcggcgcg ctcgccttcg gcatcggcac gagcgaggtc gaacacgtcc tcgccacgca
     4441 gacgctgccg cagtcgaagc cgcgcacgat gctcgtccgc acgtcgggcg cgctgcccgc
     4501 gggcgtcacg gcgaaagacc tcgcgctcgg catcatcggc gagatcggca ccgacggcgc
     4561 gaccggctac gtcatcgagt acgcgggcgc tgcggtgcgc gagctgtcga tggaagggcg
     4621 gatgacgctc tgcaacatga gcatcgagtc gggcgggcgc gccggtatgg tcgcgcctga
     4681 cgaagtgacc ttcgcctacc tcgaaagccg accgttcgcc ccgcaggggg cggcgtggga
     4741 cgaggcgctt cgctactggc aaacgctcaa gtccgacgaa gacgcgcgtt tcgaccgcgt
     4801 ggtcgaaatc gacgcttcgt cgctcgcgcc tttcgtgacg tggggcacca gtcccggcca
     4861 ggtggtcgcc gtcacgggcc gcgtgcccga cccggcggac gccgccagcg aagccgaccg
     4921 ccgcgccgcc gagcgcgcgc tcgactacat gaacctcaag ccgctgacgc gcgtcgaaga
     4981 gatcggcatc gaccgcgtct tcatcggctc gtgcacgaac gcgcgcatcg aagacttgcg
     5041 cgcggcggcg cgcgtcatcc gcggctacaa ggccgacccg aaggtgagcg cgatggtggt
     5101 gcccggctcg caggccgtga agcgcgccgc cgaggcagag ggtctcgacg cggtcttccg
     5161 cgacgcgggc ttcgactggc gcgagccggg ctgctcgatg tgtctcggca tgaaccccga
     5221 cccgctgctg cccggcgagc gctgcgcctc gaccagcaac cgcaacttcg aaggccggca
     5281 ggggcgcggc gggcgcacgc acctcgtcag ccccgcgatg gccgccgccg ccgccatcac
     5341 cggacacttc acggacgtgc gctcgtggga atacaaataa acgtcaggct cgtggtgagc
     5401 cataggacaa gcctccgcgt ttcgacgcgg ggggcattcg gatcgattcg ggtatactgc
     5461 cggcatggct gaagaccacc agaagataaa acaagtggac gtgaaaacga tggaagccgc
     5521 tctcgccaga accttccatg aacttaccgg cgcgttctac aagtgcgaca tcgccggtat
     5581 cacttacggc gacgggcgga taaacgaagg cgtcgagata accatgacac tccacgaaga
     5641 cactttcgcc gacgattcgg gcagttaaca aaaagcctcc gcgcttcgac gcgcgtgggc
     5701 attcaccatt ggcttttatg attccctttc gcacgcacaa cggcctcgtc gttccgctgg
     5761 atcagccgaa cgtggacacg gatcagatca tcccgaagca gttcttgaaa cgcatcgagc
     5821 ggacgggctt cggcgagttc ctgttctacg actggcgcta cggcgcggac ggcgagcttg
     5881 acaggtcgtt tgctttgaac gagccgcgtt atcagggcgc gagcattctc gtcgcggggc
     5941 gcaacttcgg gtgcggctct tcgcgcgagc acgcgccgtg ggcgctggcc gattacggct
     6001 tccgcgcggt catcgcgccg tcgttcgcgg acatcttcgc caacaactgc acgaagaacg
     6061 ggctgctgcc ggtcgtgctc gaagaagagg aagtggcgga gatcgtccgc cgcgcgcgcg
     6121 gcggggaggg ctaccggttg acggttgacc ttgagcgccg cgtggtggaa gatggcgagg
     6181 gctttcgggc ctccttccgg atcgacgact tccgccgcta ctgcctgctc gaagggcttg
     6241 acgacatcgg cctcacgctc aggcgcgacg ccgagatcgc cgcgtacgag tcgcgccggc
     6301 ccgcgtggcg cgccgcggtc acatcgttca acgggggtta gtcgaaaaag ggatgagaga
     6361 gtccgccgaa ttccaaacgt ccgggacgca gcgcccggac tccgttttta tgacgcagaa
     6421 gcaggtcgag tcgcgcaagc tggcgaccct cctcgaagtc agccagacgc tcgcgggcgc
     6481 gcacgacctg cgctcggcgc tgagccgcgt gctcgaaacg ctggcgagcc accacagcat
     6541 gccgcgcggc ctcgtgctcc tgctcgaacg cgagacggac gagctgcggc tcgccgccgc
     6601 tcacggcctc gccgagaacg gcgggcggcg cgccgcctcg taccgcgtcg gcgaaggcat
     6661 cacgggccgc gtcgccgaga gcggcaaggc catcatcgtc ccgcagatca gccgcgagcc
     6721 gctgttcctc gaccgcctcg gcgagcgccg caagcgccac cgcgacgagg agctgagctt
     6781 cgtctgcgtc cccgtcctgc tgggccgcaa ggcggtcggc gtgctcggcg tggacatgcg
     6841 ctacaagccc gagcgcgact tcgaccgcac caccaagttc ctcgccatca tcgcctccct
     6901 gatcgcgcag gccatcaagg tcgagcacct gatcgagtcc gacaagcaaa aactcttaga
     6961 cgagaacatc cacctcaagc aggagctgcg cgagcgctac gacttcggcc acatcatcgg
     7021 caactccggc cccctgcggc aggtctacga gcaggtgacg caggtcgcgc gcaccaacac
     7081 caccgtcctc atccgcggcg aatcgggcac cggcaaggag ctgatcgcgc acgccatcca
     7141 ctacaactcg ctgcgctccg gcaagccctt cgtcaaggtg agctgcgcgg cgctgcccga
     7201 gacgctcatc gagtccgagc tgttcggcta cgagaagggc gccttcaccg gcgcgcaagg
     7261 acgcaagaag gggcgcttcg agctggccga cggcgggacg ttatttttag acgagatcgg
     7321 cgaactcaac ctctcgacgc agatcaaact gctgcgcgcc ttgcagcagc gcgagttcga
     7381 aaggctcggc ggcacggaga cgatcaaggt caacgtccgc ctcatcgccg ccaccaacaa
     7441 ggtgctcgaa acggcgatcg ccgacggcac gttccgcgag gatctctact accggctgaa
     7501 cgtgttctcc
//
LOCUS       DBNGOH010000980         3350 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3813778, whole genome shotgun sequence.
ACCESSION   DBNGOH010000980 DBNGOH010000000
VERSION     DBNGOH010000980.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3350)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3350)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3350
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3813778"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1231)
                     /gene="asnB"
                     /locus_tag="AC159P_19100"
     CDS             complement(<1..1231)
                     /gene="asnB"
                     /locus_tag="AC159P_19100"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0070981 - L-asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HIR3697602.1"
                     /translation="MCGINGIAFSSRSGRRVEREVLERMRDCLTHRGPDDSGLYMDGR
                     VGLGHRRLSIVDVGGGHQPMTNEDDTLHIVYNGEIYNHADFRAGLEARGHRYRTHCDT
                     ETILHLYEEEGAGAVERLRGMFAFAIWDERERELFIARDRLGVKPLYYVHAADGSLYF
                     ASEIKALLAARAVAPELNHPALPDYLANHATSGEETLFRGVKRLLPGHTLRWRDGELK
                     IEKYWDLHYAAVGDGARRSDRDYVEEWAEMFRTSVRLRLMADVPLGMFLSGGIDSSAI
                     AAVMSGMVSEPIKTFSVAFAERDANELHYARMVAERFGTDHHEVVVSPEQFFEALPQL
                     IWHEDEPLAHPASVPLYFVSRLAAEHVKVVLTGEGSDEMLAGYGRYRKTVYNLALGRR
                     YQSMTPAPLRRAIAGTVR"
     gene            complement(1236..>3350)
                     /locus_tag="AC159P_19105"
     CDS             complement(1236..>3350)
                     /locus_tag="AC159P_19105"
                     /inference="COORDINATES: protein motif:HMM:NF028199.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HIR3697603.1"
                     /translation="VESAERIAAGSFDLLGLRGLSFGAPVDWHLEPLTGKRSPLVHWS
                     RIGELDAEDTGDKKIIWELNRHQHFVTLGRAYWATGDVRHARTFVAHVGSWMDQNPPK
                     LGLNWLSSLEVAFRAISWLWALHFFRDSPHLKPELFQRLLKYLYLHARHLETYLSTYS
                     SPNTHLTGEALGLYYLGLLLPEFRAARGWREAAHDILVGELARHVRSDGVYFEQSSYY
                     HRYTTDFYTHLYILSRANDGAAGGEVADKLQLLLDHLMHVTRPDGTTPLFGDDDGGKL
                     LRFEERRPNDFRAALANGAALFARADYKYVAGAAAEETLWLLGPAGLEAFDAAPAREP
                     ACLSRAFPDGGYYVMRDGWGDGANYLLLDCGPHGTLNCGHAHADALAFDLAALGRTLL
                     VDPGTCAYTASRELRDYFRGTEAHNTLTVDGEPSSVPAGPFAWRHVARAAARDWITHT
                     RFDYFEGEHDGYLRLPAPARHSRSVLFFRGDYWVVRDRVETDGAHRYSLRFHFAPGAT
                     PSVERQGDGATSVRERGGEGPGLSLFTFAAGGEDGAWREERGWVSTCYAERQSAPVVC
                     FESAGAGPQEFITFLVPSGAGASGETRVRELEAEDGRAFEVRAADGARDLLLVAVAGE
                     EAEVVTLDGGFAVKADAEWAWLRCASPDEPPAEAVLVSGRRLSAGGREIINAGRRVRY
                     ARARRVEGAWQVETGAATEGLK"
BASE COUNT          494 a         1130 c         1235 g          491 t
ORIGIN      
        1 agcggacggt gcccgcgatt gcgcggcgca gcggcgcggg cgtcatcgat tgataacgcc
       61 ggccgagcgc gaggttgtag accgtcttgc ggtagcgccc gtaaccggcc agcatctcgt
      121 cgctcccctc gcccgtgagc acgaccttga cgtgctcggc ggcgaggcgc gagacgaagt
      181 agagcggcac gctcgccggg tgcgcgagcg gctcgtcctc gtgccagatg agttgcggca
      241 gggcctcgaa gaactgctcg gggctgacga cgacttcgtg gtggtcggtc ccgaaccgct
      301 cggccaccat ccgcgcgtag tgcagctcgt tggcatcgcg ctcggcgaag gcgaccgaga
      361 aggtcttgat cggctccgag accatcccgc tcatcacggc ggcgatggcc gaagagtcga
      421 tgccgcccga gaggaacatc cccaacggca cgtcggccat cagccgcagc cgcacggagg
      481 tgcggaacat ctcggcccac tcctcgacgt agtccctgtc cgagcggcgc gcgccgtcgc
      541 ccaccgccgc gtagtgcagg tcccagtact tctcgatctt caactcgccg tcgcgccacc
      601 gaagggtgtg gccgggcagg aggcgcttga cgccgcggaa caaggtctcc tcgcccgagg
      661 tggcatggtt ggcgaggtag tcgggcagcg ctgggtggtt gagttcgggc gcgacggcgc
      721 gggcggccag cagggctttg atctccgagg cgaagtagag cgagccgtcc gcggcgtgga
      781 cgtagtagag cggcttgacg ccgaggcggt cgcgcgcgat gaagagttcg cgctcccgct
      841 cgtcccagat ggcgaaggcg aacatcccgc gcaggcgctc gacggcgccc gcgccctcct
      901 cctcgtagag gtggaggatg gtctcggtgt cgcagtgggt gcggtagcgg tggccgcgcg
      961 cttcaagccc cgcgcggaag tcggcgtggt tgtagatctc gccgttgtag acgatgtgga
     1021 gcgtgtcgtc ctcgttcgtc atcggctggt gaccgccgcc cacgtcaacg atgctcaggc
     1081 ggcggtgccc tagcccgacg cgcccgtcca tgtagaggcc gctgtcgtcc ggcccccggt
     1141 gcgtcagaca gtcgcgcatg cgctcaagca cttcgcgctc gacgcgccga cccgaccgac
     1201 tcgaaaatgc gatgccgttg atgccacaca taaacttact tcaagccctc cgtcgccgca
     1261 ccggtctcga cttgccacgc gccctcgacg cgccgggcgc gggcataccg gacgcgtctc
     1321 ccggcattga ttatctcacg cccgcccgcc gatagcctcc gcccggagac gagcaccgcc
     1381 tccgcgggcg gctcgtccgg cgaggcgcac cgcagccacg cccattccgc atcggccttg
     1441 acggcgaagc cgccgtccaa cgttacgact tcagcctctt cgcccgcgac ggcgacgagc
     1501 aggaggtcgc gcgctccgtc ggcggcgcgc acctcgaagg cgcgtccatc ctcggcctca
     1561 agctcgcgca cgcgcgtctc accggaagcc cccgcgccgc tcggcacgag gaaggtgatg
     1621 aactcctgcg gccccgcgcc cgccgattcg aagcacacga cgggcgcgct ctggcgctcg
     1681 gcgtagcacg tcgagaccca cccccgctcc tcgcgccacg cgccgtcctc gccgcccgcc
     1741 gcgaaggtaa agagcgagag gcccggcccc tcgccgccgc gctcgcgcac ggacgtcgcg
     1801 ccgtcgccct gccgctcgac ggacggggtc gcgccgggcg cgaagtggaa tcgcagcgag
     1861 tagcgatggg cgccgtcggt ctcgacgcgg tcgcgcacga cccagtagtc gcctctgaag
     1921 aagaggacgc tccggctgtg gcgcgcgggc gcgggcaggc gcaggtagcc gtcgtgctcg
     1981 ccttcgaagt agtcgaagcg cgtgtgcgtg atccaatccc gcgcggcggc gcgggcgacg
     2041 tgccgccacg cgaaaggccc ggcggggacg gacgaaggct cgccgtccac ggtgagcgtg
     2101 ttgtgcgcct cggtgccgcg gaagtagtcg cgcagctcgc gcgaggcggt ataggcgcag
     2161 gtgccggggt ctacgaggag cgtgcggccc agggcggcga ggtcgaaggc gagggcgtcg
     2221 gcgtgcgcgt ggccgcagtt gagcgtgccg tgcgggccgc agtcgagcag caagtagttg
     2281 gcgccgtcgc cccagccgtc acgcatcacg tagtagccgc cgtcggggaa ggcgcgcgag
     2341 aggcaggccg gctcgcgcgc cggcgccgcg tcgaaggctt ccagcccggc tggcccgagc
     2401 agccacaagg tctcttccgc cgcggcgccc gccacgtatt tgtagtccgc gcgcgcgaag
     2461 agggccgccc cgttggcgag cgcggcgcgg aagtcgttcg ggcggcgctc ctcgaaccgg
     2521 aggagcttgc cgccgtcgtc gtcgccgaag agcggggtgg tgccgtcggg ccgcgtgacg
     2581 tgcatgaggt ggtcgagcaa cagttggagc ttgtccgcga cctcgccgcc ggcggccccg
     2641 tcgttggccc gcgagaggat gtagaggtgc gtgtagaagt cggtcgtgta gcgatggtag
     2701 tagctcgact gctcgaagta gacgccgtcg gatcggacgt ggcgcgcgag ctcgccgacg
     2761 aggatgtcgt gggcggcctc ccgccagccc cgcgcggcgc gaaactcggg caggagcagc
     2821 ccgaggtagt agaggccgag cgcctcgccc gtgaggtgcg tgttcgggct cgaataggtc
     2881 gagaggtagg tctccaggtg gcgcgcgtgc aggtagaggt atttgagcag gcgctggaag
     2941 agttcgggct tgaggtgcgg ggagtcgcgg aagaagtgga gcgcccacag ccaggagatg
     3001 gcgcggaagg cgacttcgag cgagctgagc cagttgaggc cgagcttggg cgggttctga
     3061 tccatccagg agccgacgtg cgcgacgaac gtccgcgcgt ggcgcacgtc gcccgtcgcc
     3121 cagtaggcgc gcccgagcgt gacgaagtgc tggtgccggt tcaactccca gatgatcttc
     3181 ttgtcgcccg tgtcttcggc gtcgagttcg ccgatgcggc tccagtgcac gagcggcgag
     3241 cgcttccccg tgagcggttc gaggtgccag tctaccggcg cgccgaagga gagcccgcgc
     3301 aggccgagca ggtcgaacga gcctgcggcg atgcgttcgg cgctttcgac
//
LOCUS       DBNGOH010000981         4830 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4363376, whole genome shotgun sequence.
ACCESSION   DBNGOH010000981 DBNGOH010000000
VERSION     DBNGOH010000981.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4830)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4830)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4830
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4363376"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            147..497
                     /locus_tag="AC159P_19110"
     CDS             147..497
                     /locus_tag="AC159P_19110"
                     /inference="COORDINATES: protein motif:HMM:NF024139.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR3697604.1"
                     /translation="MSRDEAAKFLGVSVGAVNRYTARGKLQPRAGAPGGQPSYDEDEL
                     RRFKEEMDARAPAAGGGRRHRRTLSLEEAVAATGLTREFLMIRAIDGRLRARWVGSAV
                     RISRVGLEELARGP"
     gene            693..1019
                     /locus_tag="AC159P_19115"
     CDS             693..1019
                     /locus_tag="AC159P_19115"
                     /inference="COORDINATES: protein motif:HMM:NF018894.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HIR3697605.1"
                     /translation="MSTNYRRTPRVRTSIHVQLDVTRHYAFRGTILSLSTRGCLIETC
                     VAEQLQGRTVFLRLLALSQESLSLQGKAIYLYEGKCGVEFTDLTSKEKGLLVELVRHH
                     RVKANK"
     gene            complement(1271..1723)
                     /locus_tag="AC159P_19120"
     CDS             complement(1271..1723)
                     /locus_tag="AC159P_19120"
                     /inference="COORDINATES: protein motif:HMM:TIGR04354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amphi-Trp domain-containing protein"
                     /protein_id="HIR3697606.1"
                     /translation="MQKTALEVAAALRRLAEAVEGGTSAVLELAGENVFVPSDADVKV
                     VFERGGGAREVNIRVTWGLVSAPPLLIHRHSEQVQDSGGHMYDVLVYGEPRADGMWEG
                     WLEFHPLSAALGTRRTGRETTQPDRAALEYWATGLEPLYLSGAFERAV"
     gene            complement(1824..2024)
                     /locus_tag="AC159P_19125"
     CDS             complement(1824..2024)
                     /locus_tag="AC159P_19125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697607.1"
                     /translation="MGTANRIKDAAIRTRRLARIVLSNDVEYSAEERARVRAALNHAP
                     HTLPRVLLDLHKEPRGSDRAAA"
     gene            2437..2949
                     /locus_tag="AC159P_19130"
     CDS             2437..2949
                     /locus_tag="AC159P_19130"
                     /inference="COORDINATES: protein motif:HMM:NF019430.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0044183 - protein folding chaperone
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HIR3697608.1"
                     /translation="MKKIFAFASFAVVLAAAGAIYTVHASGAEAARWERAVFAQDDGH
                     GGHFKHRVVGRIMAHVARELKLTEAQQTEIHAVFAAERRNVLPLLQRLREGKQQYDSA
                     NAPGSFDEAKARAFASRQAETIAELLVAKERVKSKVYAVLTPEQREKANTMHERFAAR
                     VHEHFQSAGL"
     gene            3259..>4830
                     /locus_tag="AC159P_19135"
     CDS             3259..>4830
                     /locus_tag="AC159P_19135"
                     /inference="COORDINATES: protein motif:HMM:NF012618.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HIR3697609.1"
                     /translation="MTKDQSTGGSVSAFGLFSKLHGINTGSLLLFAALLSPFAAPLAR
                     ARQQDERGIVVRQTGQGRATGQPRRQGGARPELVLQTGYGNFLGATRFVFSPDARLLA
                     TGAFNDTNVKLWEVSTGRELRVLPGRAAGTYTVPAIAFSRDSRLVATSGGENTIKVWE
                     IATGREVQTFAGPAGGLLSMAGVSFISFAADGRTLVAVSDAIRTWDVATGAERSVADL
                     SALGMGGLGTSASASFGVGSGFALSPDGAQLAVVSVEGGSKVRVRLLDTATGREARSL
                     NLSGVDKPHELYRAFPAFARDGRLVVAGVDGGKLKVWDAAGKPQTLASVPADTGFVGL
                     SPGGDVVAAALADYKLKAWDAATGREVVSLDVPHGGIYKGAPIFAFGFSHDARLLATG
                     GLDAQPALWDVSSGKQLRALEGHSNVAYNVAFSADGTRLTSGARTVWDLRTGQGLRVV
                     PGAERIYGFPTADGKLLAVITLGASALRVVETATGRTLHTLAPAGGGGLALQFASSLD
                     GRLLAVYYPQTTEETE"
BASE COUNT          772 a         1630 c         1656 g          772 t
ORIGIN      
        1 agggcggcgc cgctatgatg cccgcacgtt ctttcttcct taagtggtcg atatgaacat
       61 tggggcaggc taccgatgta gccggaggcg ccgggcttgg gggagtccgg cgcctttcct
      121 ttaacgacag gcgtggaggc gattgggtga gtagggacga ggccgcaaag ttcctcggcg
      181 tgagcgtagg cgcggtcaac cgctacacgg cgcgcggcaa gctgcagccc cgcgcaggcg
      241 cgcccggcgg gcagccctcc tacgacgagg acgagttacg caggttcaag gaggagatgg
      301 acgcgcgcgc gcccgcggcg ggcggggggc ggaggcacag gcgcacgctc tcgctcgagg
      361 aggcggtcgc ggcgacgggg ctgacgcggg agttcctgat gattcgcgcc atcgacggca
      421 ggctgcgcgc gcggtgggtc gggagcgcgg tgaggatcag tcgcgtcgga ctcgaagagc
      481 tggcgcgcgg cccctagggc gtactacctc gccgtcaagc tgttctgctg ccagttcggc
      541 gacgaaaacc gttacccccg ccctctccgg gagtagagtc cgcacgaaac tctcctgggg
      601 atgcactcct ttccactcga ttcatctccc gcccagccgc tcgggtacgt aagatttcta
      661 atgtcatcca cagacgcgct aaactacacg gcatgtccac caactacagg cggacgccac
      721 gcgttcgcac ctccatccac gtgcaactgg acgtgacaag acactacgcg ttccgcggga
      781 cgatcctcag cctgtccacc agggggtgcc tcatcgagac ctgcgtggcg gaacaactcc
      841 aaggccggac cgtcttcctc cgcctgctgg cactctcaca agagtcgctg tcactgcaag
      901 gcaaggccat ctacctgtac gaggggaagt gcggcgtcga gttcaccgat cttaccagca
      961 aggagaaggg gctgctcgtc gaactggtgc ggcaccaccg ggtgaaagcg aataaatagc
     1021 tctcctcata gatcacctta cggagtcggg agaggcgacg gtgcacgagc ttgtagctag
     1081 cggcttgatc tattctgccc ggcagcagcc ggacacgggt gggaggccgg tatcggggcg
     1141 cgtctggggg cggcccgcgc cgtgcgggcg cacgccgtga ggggtatggc gtgcgcgcgg
     1201 gcgcgggaga ccgttcagcg cctccatatg tgcacaggca cttcgagccg cgggccggga
     1261 cgcgcacacc tcagaccgcg cgctcgaatg cgccggacaa gtaaagtggc tccaagccgg
     1321 tcgcccagta ctccaacgcg gcccggtccg gctgcgtcgt ctcgcgcccg gtgcgacggg
     1381 tgccgagtgc cgcgctcaag gggtggaact caagccagcc ctcccacatg ccgtccgcgc
     1441 gcggctcgcc ataaacgagc acgtcataca tgtgtccccc ggagtcctgc acctgctcgc
     1501 tgtgccgatg gatcaggagc ggcggagccg acaccagccc ccacgttacc ctgatgttca
     1561 cctccctggc cccgccgcca cgctcgaaca cgaccttgac atcggcgtcg gacggaacga
     1621 aaacgttctc gccggccagt tccagcacgg cagacgtgcc gccctcgacg gcttcggcga
     1681 ggcggcggag tgcggcagcg acttctagcg ccgtcttttg catatttttt gacctccgtc
     1741 tgaattcgaa ggggttgagg ggcacttatt gccgggggat ccgaaacggg gcggcgtggc
     1801 cgggctagcg cggggcttca ggcttaggcc gccgcccggt cgctcccccg aggctccttg
     1861 tgcagatcga gcaggacgcg cggcaaagtg tgcggggcgt ggttgagcgc ggcgcgcaca
     1921 cgcgctctct cctccgcact gtactccacg tcatttgaca ggacgatgcg cgcgagcctg
     1981 cgcgtgcgga tggcggcatc cttgatgcgg ttcgctgttc ccattgggta gacctttccg
     2041 gggaaggttc ggggcaggta gggggccggg gatagtcata ccctactccg cggccccccg
     2101 tttgtcacgc cctcctcccg ttcttccgcg tctcacgctt cggttcacgg atgcggggcg
     2161 atctcgaagt cggggtctgc ggcgggttgt gcggcgacaa tcttaaagtc ggcgccgagc
     2221 ggttcgcggg ggtggtaagc cgcagcgggc cgttcgtggt agcttgttgc cccgcgggcg
     2281 cgtggccctt aacgaaactt tacgcgcgct tcaccactct taaccgcacg gcgggcggcg
     2341 gtcgcgtata aacgtggcga gagacgaggc gcgccgctat cgtcgtcaag gacaacggcg
     2401 cagacgataa agcttttcga gaggagaaga agagagatga agaagatatt cgccttcgcg
     2461 tcgttcgccg tcgtgctcgc cgcggcgggc gcgatctata ccgtccacgc ttccggcgcg
     2521 gaggccgcgc gctgggagcg cgccgtcttc gcgcaggacg acggacacgg cggccacttc
     2581 aagcaccgcg tcgtgggccg catcatggcg cacgtcgcgc gcgagctgaa gctgaccgag
     2641 gcgcagcaga ccgagattca cgccgtcttc gccgccgagc gccggaacgt gctgccgctc
     2701 ctgcaacggc tccgcgaggg caagcagcag tacgactccg cgaacgcgcc cggcagcttc
     2761 gacgaagcga aggcgcgcgc cttcgcctcg cgccaggccg agaccatcgc cgagctgctc
     2821 gtcgccaagg agcgcgtcaa gtcgaaggtc tacgccgtgc tcacgcccga gcagcgcgag
     2881 aaggccaaca cgatgcacga acgcttcgcc gcccgcgtcc acgaacactt ccaaagcgcc
     2941 gggttataac ggaaggggcg aaagggttaa caggggaaag ggcaaaaggg gaagagggag
     3001 gacgagaacc gcgctgctcg ccatcctcgg tcgagaacga aaaacagcgt gcctctcatc
     3061 ttcagtcgaa gatgagaggc acgctgtttt tcatcctccc ttttcgcccc ttcccctttg
     3121 acccttttcc cctcttcccc tcttccccct cggcctttca acctgttcgc ccatgaatta
     3181 gactaggcgc tctccttcgg aacacacttg atcccttccg gcgcgagaag ccgcgttagt
     3241 cttcgggagc tgcgcgaaat gacgaaagac caatcgaccg gcgggagcgt ttccgccttc
     3301 ggcctcttct cgaaactgca cggcatcaac acgggttcgc ttctgctctt cgccgccctg
     3361 ctctctccgt tcgcggcgcc cctcgcgcgc gcgcggcagc aggacgagcg cggcatcgtc
     3421 gtcaggcaga cggggcaggg ccgggcgacg ggccagccgc gccggcaggg cggggcgcgg
     3481 cccgagctgg ttttgcagac gggctacggc aacttcctcg gcgcgacgcg cttcgtcttc
     3541 agcccggatg cgcggctgct ggcgaccggc gccttcaacg acaccaacgt caagctgtgg
     3601 gaggtctcga cggggcgcga gctgcgcgtg ctccccggca gggccgccgg cacttacacg
     3661 gtgcccgcca tcgccttcag ccgcgacagc cgcctcgtcg cgacgagcgg cggcgagaac
     3721 acgatcaagg tctgggagat cgccacgggg cgcgaggtgc agacgttcgc ggggcctgcg
     3781 gggggcttgc tgtcgatggc gggcgtctcc ttcatctcgt tcgcggcgga cgggcgcacg
     3841 ctcgtcgccg tcagcgacgc gatcaggacg tgggacgtgg cgaccggcgc cgagcgcagc
     3901 gtcgcagacc tctccgcgct cggcatgggc ggactgggca cttccgcctc ggcctcgttc
     3961 ggcgtcggct ccggtttcgc gctcagcccc gacggcgcgc agctcgccgt cgtctccgtc
     4021 gaaggcggct cgaaggtgag ggtcaggctg ctcgacacgg cgaccgggcg cgaggcgcgg
     4081 agcctgaacc tgtcgggcgt cgataaaccg cacgagctgt acagggcttt ccccgcgttc
     4141 gcgcgcgacg ggcgactggt ggtggccggc gtggacggcg ggaagctcaa ggtgtgggac
     4201 gcggcgggca agccgcagac gcttgcctcc gtccccgccg acaccggttt cgtcggcctc
     4261 agccccggcg gcgacgtggt cgcggcggcg ctcgccgact acaagctcaa ggcgtgggac
     4321 gcggcgacgg ggcgcgaggt cgtgtcgctc gacgtgccgc acggcggcat ctacaagggc
     4381 gcgccgatct tcgccttcgg cttcagccac gacgcgcggc tcttggccac cggcggcctc
     4441 gacgcgcagc ccgcgctctg ggacgtgtcg agcggcaagc aactgcgcgc gctcgaaggc
     4501 cacagcaacg tcgcctacaa cgtcgccttc agcgccgacg ggacgcgcct cacttcgggc
     4561 gcgcgcaccg tctgggactt gcgcaccggg cagggcctgc gcgtcgtccc cggcgcggag
     4621 cgcatctacg gcttccccac cgcggacggc aagctcctcg ccgtcatcac cctgggcgcg
     4681 agcgcgttgc gcgtcgtcga gaccgcgacc gggcgcacgc tccacacgct cgcgcccgcg
     4741 ggcggcggcg ggctggcctt gcagttcgct tcgagcctcg acgggcggct gctcgccgtc
     4801 tactacccgc agacgaccga ggagacggag
//
LOCUS       DBNGOH010000982         6155 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2347881, whole genome shotgun sequence.
ACCESSION   DBNGOH010000982 DBNGOH010000000
VERSION     DBNGOH010000982.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6155)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6155)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6155
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2347881"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(151..588)
                     /locus_tag="AC159P_19140"
     CDS             complement(151..588)
                     /locus_tag="AC159P_19140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697610.1"
                     /translation="MREIFGGRARADSKTGEGVAMFMRFVVTQIDEDSRQPQGVFSAA
                     HALLDSGRLDADEERRLREVLSWFEENLPAPSEHFRAWRAVFWFKAGAREQVCQAWGL
                     ACLLRLHGLHVEVYKCRRLANLVYEDAGQVAAYPSKRDGRIAR"
     gene            complement(603..1235)
                     /locus_tag="AC159P_19145"
     CDS             complement(603..1235)
                     /locus_tag="AC159P_19145"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017716752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HIR3697611.1"
                     /translation="MSIFYHEQLERSAEVMRRLREQPVTVCGAGALGANVAESLARAG
                     FGRLRVIDRDRVEERNLSTQPYYRSDVGAQKAKLLAHMLYRALGVEVESRAEELDARN
                     AERLLDGVVVDAFDNSASRRAVKEACEVKGLACLHAGLAGGYAEVIWNEQYRVPSAAQ
                     DDVCDYPLARNLVTLAVAVACEAVVRYVAAGERESYTLTLGDFAVRRFGA"
     gene            complement(1363..2232)
                     /locus_tag="AC159P_19150"
     CDS             complement(1363..2232)
                     /locus_tag="AC159P_19150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009456975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697612.1"
                     /translation="MTDVRDIVRRLAEAEEGLRARRFLAPCVRGGGVRASVGGLVYTF
                     APAPRDFEGWAVFRPADERTARVVEEAELPRVAEYLNLFPSLRVRLAYSLRGATWVAC
                     PANAGEAQRRFPSLRAWPPVVHLVCDGREFEQVVVRAVGGAWLFEDADRRADPLDAER
                     LREAWRQRLQPGALDWKGCTPEMRACYALPFAREMQTREQRRLREERSRRGRDETRLR
                     EALRFGGGDLGSYRDRGDHWLVEWMARDGESHTSAISKRDLTVVSAGICLSGEDDKFD
                     LHSLVGVVAGAWE"
     gene            complement(2352..3788)
                     /locus_tag="AC159P_19155"
     CDS             complement(2352..3788)
                     /locus_tag="AC159P_19155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015217363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697613.1"
                     /translation="MTQRNERDLRLGMLNSLLTTPHRELGQVAGMHREMVERDPVFYG
                     HLAAWYFRSGDVRDHKEVFVGNLLTGALDEHRGAGFVLLQQLPPYQVARVVDFVKQTR
                     GGVPRSTRTAVVRYLREREAEPERFDRAALRARKSMKRLYAGLHIRPDARADAVLFKD
                     EPPEASLAFKLKQLAKAGSAAEQAALIVEHRLPYAVAVGAVGKLTPSVLVALISRMSP
                     QEVINNLGSLKKRGALEHAEVKALVDERLKAAGTSERVSAFKALKAAEVAGVGEETVA
                     KLGAVAGEQVKRKGRITRSTALFVDKSSSMTEAVEVGKQIAALVSGVAEAELYVYAFD
                     TMAYPVAPAGSGRELADWERAFRHVRAEGCTSIGAPLEVMRLKKQSVEQVIIVTDEGE
                     NTAPFFTSVLPRYCEELKADPSVLIVKVGQHCDHLENHLRGARRAFDTFTFGGDYYSL
                     PNLVPLLSRPSRLELLLEILETPLPVRE"
     gene            complement(4141..4213)
                     /locus_tag="AC159P_19160"
     tRNA            complement(4141..4213)
                     /locus_tag="AC159P_19160"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4178..4180),aa:Ser,seq:tga)
     gene            complement(4886..5086)
                     /locus_tag="AC159P_19165"
     CDS             complement(4886..5086)
                     /locus_tag="AC159P_19165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016865358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697614.1"
                     /translation="MVHIRYDGRSVDVGEQQLGVSAGMTDREIKQQVARHFDVGAKRL
                     DLYVVDRTPNGDLVVRPEAVYG"
     gene            complement(5431..5664)
                     /locus_tag="AC159P_19170"
     CDS             complement(5431..5664)
                     /locus_tag="AC159P_19170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697615.1"
                     /translation="MSTKSNAARRGKARASARPANETIACVFMDHLFVVCQCRESRGA
                     AAAACPYSLETPTTPKPAAREGVRLPALLCNKK"
     gene            complement(5707..6117)
                     /locus_tag="AC159P_19175"
     CDS             complement(5707..6117)
                     /locus_tag="AC159P_19175"
                     /inference="COORDINATES: protein motif:HMM:NF019498.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR3697616.1"
                     /translation="MEATSDVNSHPLHTDWSRVPVERLEEGVERQMIVGERVMVCRLR
                     FAPRVVTPPHDHPHEQLTFVERGRVLFTVGEVERVAEAGDVLHFPPGTWHGATMLDEE
                     VVLIDIFSPIREDFLGASRLYGNGDNGDGRAPRG"
BASE COUNT          980 a         2213 c         1925 g         1037 t
ORIGIN      
        1 ggcgagtttt gcaaaacgtc cggccccgac gtttttcgat tttgtgcgga gaaaagggcc
       61 gaccgcaggt tactgcggcg gccttgaaaa aagcttgacg tgcggcggcc ccgccagcgg
      121 cggcggcccg gaggcgcggc ggcgacaagc tcaacgcgcg attctgccgt ccctcttcga
      181 agggtacgcg gccacctgcc ccgcgtcctc atagacgagg ttggcgagac ggcggcactt
      241 gtaaacctcg acgtgcaggc cgtgcaagcg cagcaggcag gccagccccc acgcctgaca
      301 gacctgctcc cgcgcccccg ccttgaacca gaagaccgcc cgccacgcgc ggaagtgttc
      361 ggacggcgcg ggcaggtttt cctcgaacca cgagagcact tcgcgcagcc gccgctcttc
      421 gtccgcgtcc aaccgccccg agtccaacag cgcgtgggcg gcggagaaga cgccctgcgg
      481 ctgccgcgag tcctcgtcta tctgtgtgac cacgaaacgc atgaacatgg cgaccccttc
      541 gcccgtcttg gaatccgcac gcgcgcgccc gccgaatatt tcgcgcacgc ccgattttca
      601 cctcacgccc cgaagcgcct gaccgcgaag tcgccgagcg tcaacgtgta gctctcgcgc
      661 tcgcccgcgg cgacgtacct gacgacggcc tcgcacgcga ccgcgaccgc gagcgtcacg
      721 aggttgcgcg cgagcgggta gtcgcacacg tcgtcctgcg ccgccgaagg cacgcggtac
      781 tgctcgttcc agatcacctc cgcgtaaccg ccggcgaggc ccgcgtggag acaggccagt
      841 ccctttacct cgcaagcctc cttcaccgcc cgccgcgacg cgctgttgtc gaacgcgtcc
      901 acgacgacgc cgtcgagcag cctctccgcg ttgcgcgcgt ccagttcctc ggcccgcgac
      961 tcgacctcca cgcccagcgc gcggtagagc atgtgcgcca gcagcttcgc cttctgcgcg
     1021 cccacgtccg agcggtagta cggctgcgtc gagaggttgc gctcctccac gcggtcgcgg
     1081 tcgatcacac gcagccgccc gaaccccgcg cgcgccagac tctccgcgac gttcgcgccg
     1141 agcgcccccg ccccgcacac cgtcaccggc tgctcgcgca acctccgcat cacctccgcg
     1201 gagcgctcta gctgttcgtg gtagaagatc gacataaaaa cagtaggcag taggcagtag
     1261 gcagttaaaa acctttctct gtcgaacagt cagggtgcag gcgtcaacgc cgtcctcagt
     1321 caactgactg cctactgcct gctgcctact gcctactgtt gttcattccc acgcccccgc
     1381 gacgacgccg acgagcgagt ggaggtcgaa cttgtcatcc tcgccgctga ggcagatgcc
     1441 ggcgctgacg accgtgaggt cgcgcttgga gatggccgag gtgtgcgact cgccgtcacg
     1501 cgccatccac tcgacgagcc agtggtcgcc gcggtcgcgg taggagccga ggtcgccgcc
     1561 gccgaagcgc agcgcctcgc gcagccgcgt ctcgtcgcgc ccgcggcggc tgcgctcttc
     1621 acgaaggcgc cgctgctcgc gcgtctgcat ctcgcgcgcg aagggcagcg cgtagcacgc
     1681 gcgcatctcc ggcgtgcagc ccttccagtc gagcgcgccg ggctggaggc gttgacgcca
     1741 cgcttcgcgc agacgttccg cgtccagagg gtctgcgcgg cggtctgcgt cttcgaagag
     1801 ccacgcgccg ccgaccgcgc ggacgacgac ctgctcgaac tcccgcccgt cgcacacgag
     1861 atggacgacg ggcggccacg cgcggagcga cgggaaacgc cgctgcgctt cccccgcgtt
     1921 cgcggggcac gcgacccacg tcgcgccccg caggctgtag gcgagccgca cgcgcaggct
     1981 cgggaaaaga ttcaggtact ccgcgacgcg cggcagttcc gcctcttcca cgacgcgcgc
     2041 cgtgcgctcg tccgcgggtc ggaagacggc ccagccctcg aagtcgcgcg gcgcgggcgc
     2101 gaacgtgtag acgaggccgc ccacgctcgc acgcaccccg cccccgcgca cgcacggcgc
     2161 gagaaaacgc cgcgcacgca agccctcctc cgcctccgcc agacgcctca cgatgtcacg
     2221 aacgtccgtc atcttccctc gaaacagtga gcagtcagca gtgagcggta aaagacagaa
     2281 gaaagaagag gcttgggatt gaacgtctca agcctcttcc ctctcactgc tcaccgctca
     2341 ccgctcgctg cttactctct gaccggcagc ggcgtctcca gaatttcgag cagcagttcg
     2401 agccgcgaag gccgactgag cagcggcacg aggttcggga gcgaatagta gtcgccgccg
     2461 aaggtgaacg tgtcgaacgc gcggcgcgcg ccgcgcaggt ggttctcaag gtggtcgcag
     2521 tgctggccga ccttgacgat gagaaccgaa gggtctgcct tcagctcttc gcagtagcgc
     2581 ggcagcaccg acgtgaagaa cggggccgtg ttctcgccct cgtccgtcac gatgatgacc
     2641 tgctcgaccg actgcttctt cagccgcatc acttcgagcg gcgcgccaat gctcgtgcag
     2701 ccctcggcgc ggacgtggcg aaaggcgcgc tcccagtcgg ccagctcgcg tccgctccct
     2761 gcgggcgcga ccgggtaagc catcgtgtcg aaggcgtaga cgtacagctc ggcctccgcg
     2821 acgcccgaga cgagcgccgc gatctgcttg ccgacctcga cggcctcggt catgctgctg
     2881 ctcttatcga cgaacagcgc ggtcgagcgc gtgatgcgac ccttgcgctt cacctgctcg
     2941 cccgcgaccg cgccgagctt cgccacggtc tcttcgccca cgcccgcgac ctccgccgcc
     3001 ttcaacgcct tgaaggccga gacgcgctcc gacgttcccg ccgccttcag ccgctcgtcc
     3061 acgagcgcct tcacttcggc gtgctccagc gcgccgcgct ttttcagcga gccgaggttg
     3121 ttgatgacct cctgcggcga catccggctg atgagcgcga cgagcaccga cggcgtcagc
     3181 ttgcccacgg cgccgaccgc caccgcgtac ggcaggcggt gctcgacgat cagcgcggcc
     3241 tgctccgccg ccgagccggc cttcgccaac tgcttcagct tgaacgcgag gctcgcctcc
     3301 ggcggctcgt ccttgaacag cacggcgtcg gcccgcgcgt cgggcctgat gtgcagcccc
     3361 gcgtagaggc gcttcatgct cttgcgcgcg cgcagggccg cgcggtcgaa ccgctccggc
     3421 tcggcctcgc gctcgcgcag gtagcgcacg accgccgtgc gcgtcgagcg cggcacgccg
     3481 ccacgcgtct gcttcacgaa gtccaccaca cgcgccacct gatacggcgg caactgctgt
     3541 aacagcacga agcccgcgcc gcggtgctcg tcaagcgcgc ccgtcaacag gttgccgacg
     3601 aacacctcct tgtggtcgcg cacgtcgccg ctccggaagt accacgcggc caggtgcccg
     3661 tagaacacag ggtcgcgctc gaccatctcg cggtgcatcc ccgccacctg ccccagctcg
     3721 cggtgcggcg tcgtcaacag actgttcagc atccccaacc gcagatcgcg ctcgttacgc
     3781 tgcgtcatca cttcacctcc acaaaccaag tgctgaggac tgaggactga ggactgagtg
     3841 aaaaacatct tgctttactc aaacctcggt cctcagcact cctttatttg ccgcgcaagt
     3901 cgcacgggct atgtgccggc ttaaggcccc ggctcgcagc gcagtttctc aggctgcgcg
     3961 ccttttacat ggtgagagtg tgtaagcccg tgccgtcggg gcgcggcaat ctcgtttcga
     4021 aagccgtgaa ccgtaaaccg taaaccgtga tgagtatgaa actggttctg actattcacg
     4081 gtttacggtt cacggttcac ggcttttcct gctgccccgc gcgagtcgtg cgaacgtcag
     4141 gcacatacgc gggagttgaa cccgcgacct tccgatctca agtcagaggc tctaccgagt
     4201 gagctagtat gtaagtccgc gcagtcgggg cgcagggcag ccggaaaatt cagtgtgtgc
     4261 tccgtgcaag tcgtcgaagc ttgcttcact acgctcaggg cgttttccgt ttacgccacg
     4321 ggcgctttcg gggcgcccgg tgggactcga acccacatag acctttcggt cgctgagtca
     4381 ccattgagtg tgtacgcttc ggcggtcggg gcacggagcg cccgagcctt atcggtgcgt
     4441 cgttgattcg aaagagtttc gtcgcctcgg gtctcggcgt cgcacgtgtg ccgccgtgcg
     4501 gcggcgcgtg caagttcgga aagctgttcg tagtcgcccc cgatcttttg gaggaggcgg
     4561 gaggattcga acctccctta acggttttga gccgtctttc cggtgtgtaa gctttgcaag
     4621 tcagggcacg cgccgcacgc acggtgcggc acgcgtccga ctccgaaacc cttcgggttg
     4681 atcggcgcgt ccccgcgcaa gtcgaaaaag cgttttcacg acgttggtgc tctacctctg
     4741 agctacgggg gcagaaagtg ccttacgccc ccggcgggaa tcgaacccgc ggccacccgc
     4801 ttatcaggcg atagtgtgta agcttctcgc ggtcggggcg cggggacgcg ccgaactttt
     4861 caaagacctg agactgcggg acgcctcagc cgtagaccgc ctcggggcgc acgacgaggt
     4921 cgccgttcgg cgtgcggtct acgacgtaaa ggtcgagcct cttcgcgccc acgtcgaagt
     4981 ggcgcgcgac ctgctgcttg atctcgcggt cggtcatgcc ggcgctcacg ccgagctgct
     5041 gctcgcccac gtcaaccgag cgaccgtcgt aacggatgtg aaccatctcc tcttcctccc
     5101 tttcgcgcac acgaaaaaag cgggccttcg tcgtctcctc cgttcggaga ccgaagcccg
     5161 ctcccgcggc gcaccctgaa acccctgttc gagaatgccg ggagcggagg cccccggcca
     5221 ccgctgcgtc agattttttc cggatagaag ccgggcgctg cgtcgcgccg ttcggcccgc
     5281 gccggcagag acaacggcca cgcgccgccg ccccgccgtc cgttccccga tgtctctctc
     5341 gccttcatcc ttactcttcc cttcccttcg gcccgcgcgc cctctccggc gccggacgat
     5401 ctttatacgc caacctctca ttcctgtcaa ctacttttta ttacaaagta gggcgggcag
     5461 ccgcacgccc tcgcgcgcgg cgggcttcgg cgtggtcggc gtctcaaggc tgtaggggca
     5521 cgcggcggcg gcggcgccgc gcgactcgcg gcactggcac acgacgaaga ggtgatccat
     5581 gaagacgcag gcgatggtct cgttcgccgg ccgcgccgag gcgcgcgcct tcccccgacg
     5641 agccgcgttt gatttggtgg acaacgtttc cctccgtccc ggtctacagc cgccttagat
     5701 tcggagctag ccgcggggcg cgcgcccgtc gccgttgtcg ccgttgccgt agagccgcga
     5761 cgcgccgagg aaatcttcgc ggatgggcga gaagatgtcg atgaggacga cctcttcgtc
     5821 gagcatggtc gcgccgtgcc aggtgccggg cgggaagtgg agcacgtcgc cggcctccgc
     5881 gacgcgctcc acttcgccga cggtgaagag cacgcgcccg cgctcgacga aggtcaactg
     5941 ctcgtgcggg tggtcgtgcg gcggcgtgac gacgcgcggc gcgaagcgca ggcggcagac
     6001 catcacccgc tcgccgacga tcatctgccg ctcgacgccc tcctccagcc gttcgaccgg
     6061 gacgcgcgac cagtccgtgt gcagcgggtg cgagttcacg tccgaagttg cttccatgct
     6121 ctaccgccct ttcccttccg ccttgactgc gatta
//
LOCUS       DBNGOH010000983         1748 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3630467, whole genome shotgun sequence.
ACCESSION   DBNGOH010000983 DBNGOH010000000
VERSION     DBNGOH010000983.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1748)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1748)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1748
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3630467"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..838
                     /locus_tag="AC159P_19180"
     CDS             <1..838
                     /locus_tag="AC159P_19180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697617.1"
                     /translation="LREARHLLRDRSYRRFLTEREAALFDADAVWVESGPLTSGVQVM
                     LAPEAPQWCVYMPEFFNSAAGRARFARAVESARGAKIYSLCLAEDFKSATDYIRGAGL
                     GVGKVTGLSLPFYSRAQRFHTSFLIEVLPPGAADGGAAKQVTAADAALLDHAFKASLR
                     WAQPPPANMRAGETRALRVAVTNASNLPWPALGRSDNSFRLFLGNHWLDTAGASVIND
                     DGRASLPHDLMPGEEVELTLDITAPRAPGEYQLEVDLLQERAAWFGLKGSKTLRTKVT
                     VE"
     gene            1023..>1748
                     /locus_tag="AC159P_19185"
     CDS             1023..>1748
                     /locus_tag="AC159P_19185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697618.1"
                     /translation="MPTTRGLFFERDWMKGFDNRAGAFLSLAAFVAAFALLWLHARRY
                     LPFLSDDALISLRYAKRLAGGHGLTWNAGERVEGYSNLLWVLCAAALNRLGVDLIDAV
                     RVLGFAGVGAALAAVLYAHPPRSLKGTVPVLLALLFVPLSGAMAVWAVGGMEQPLVAA
                     LLAWGVVLCYPLLEKRDATFKEMLRPGTCFALLCWARLDSPVFVAAAVAAIFLIDGLN
                     RDALRKATGLALLPVVFCLLQVAF"
BASE COUNT          252 a          592 c          603 g          301 t
ORIGIN      
        1 cctgcgcgag gcgcgacact tgctgcgcga ccgctcctac cggcggttcc tcacggagcg
       61 cgaggcggcg ctcttcgacg cggacgccgt ctgggtcgag agcggcccgc tgacgagcgg
      121 cgtgcaggtc atgctcgccc cggaagcgcc gcagtggtgc gtctacatgc cggagttctt
      181 caactccgca gcagggcgcg cgcgtttcgc ccgcgcggtc gagagcgcgc ggggcgcgaa
      241 gatttattcg ctctgcctcg ccgaagactt caagtccgcc accgactaca tcaggggcgc
      301 gggcctcggc gtcggcaagg tcacgggcct ctcgctcccc ttctactcgc gcgcgcagag
      361 gttccacacc tccttcctca tcgaagtgct gccgcccggc gccgcggacg gcggcgccgc
      421 gaagcaggtc acggcggcgg acgcggcgct gctcgaccac gctttcaaag cctcgctccg
      481 ctgggcgcag ccgccgcccg cgaacatgcg cgcgggcgag acgcgggcct tgcgcgtcgc
      541 cgtcaccaac gcgagcaacc tgccctggcc cgcgctcggg cgaagcgaca acagcttccg
      601 cctcttcctc ggcaaccact ggctcgacac ggcgggcgcg agcgtcatca acgacgacgg
      661 acgcgcctcg ctcccgcacg acctcatgcc cggcgaagag gtcgagctga cgctcgacat
      721 cacggccccg cgcgcgcccg gcgaatacca gctcgaagtc gatctgctgc aagagcgcgc
      781 cgcgtggttc ggcctgaagg ggtcgaagac tttgaggacg aaggtgacgg tcgagtaatg
      841 aagagttttc ttctgcggct gtgccgcctg atgtgcggtg aggctcggcc tcaccgggag
      901 ggctactgaa taagagttga ggcgcagcct caactcaggg gcatagcccc gaactcaaca
      961 aacacgcaat taccggaagg gcacagccct tccgcacatc aggcggcaca gccgcggaga
     1021 agatgcctac cacaagaggt ctatttttcg aacgcgattg gatgaaaggc ttcgacaata
     1081 gagctggtgc tttcctcagt ctcgccgcct tcgtcgcggc gttcgcgctg ctgtggctgc
     1141 acgcgcggcg ttacctgccg ttcctgtcgg acgacgcgct gatctctctg cgctacgcga
     1201 agcgtctggc cggggggcac gggctgacgt ggaacgcggg cgagcgcgtc gagggctatt
     1261 cgaatctgct gtgggtcttg tgcgcggcgg cgctgaaccg gctcggcgtg gacttgatcg
     1321 acgcggtgcg cgtgctcggc ttcgcgggcg tgggcgcggc gctagccgcc gtgctctacg
     1381 cgcacccgcc gcggtctttg aagggcacgg tgcctgtctt gctcgcgctg ctcttcgtgc
     1441 cgctgtcggg cgcgatggcg gtgtgggcgg tcggcgggat ggagcaaccg ctggtcgcgg
     1501 cgctgctcgc gtggggcgtc gtgctctgct acccgctgct ggaaaaacgc gacgcgacgt
     1561 tcaaggagat gctgcggccc ggcacctgct tcgcgctgct ctgctgggcg aggctcgaca
     1621 gccccgtctt cgtcgcggcg gcggtcgcgg cgatttttct gatcgacggg ttgaaccgcg
     1681 acgcgcttcg taaagcgacc ggcctggcgc tgctgcccgt cgtcttctgc ctgctgcaag
     1741 tcgccttc
//
LOCUS       DBNGOH010000984         1804 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_148797, whole genome shotgun sequence.
ACCESSION   DBNGOH010000984 DBNGOH010000000
VERSION     DBNGOH010000984.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1804)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1804)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1804
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_148797"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1291)
                     /locus_tag="AC159P_19190"
     CDS             complement(<1..1291)
                     /locus_tag="AC159P_19190"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR3697619.1"
                     /translation="MERESLEMDVVFVGAGPANLSGALHLARLVKQHDELIAKGERQG
                     KPLGELQIAVIEKGASVGAHILSGAVMDPKALRELIPDFESEGAPLESPVKEDYFLYL
                     TKTRAIKSPITPPPLKNHGYYIVSLNKLTAWLGEKCEEAGVNIFPEFPGAEMLYDEQD
                     RVVGVRTGDKGVDREGRRRANYEPGVDLLAKVTVLGEGPRGSLAKQLTQRLNLNDGRE
                     PQVYSLGVKELWELPDDRYPAGRVTHTLGYPSDASTYGGGWIYGMQNRIVNLGYVTGL
                     DYRDPLLDPHAEFQRFKQHPFVAKLLEGGKMIRYGAKTITAGGFYAIPQFHGDGFLLA
                     GDCAGFLNSQRLKGIHTAIKSGMLAAQTIFEALLNEDYSKQQLKRYRQLIRESWITQE
                     LRRVRNFHAAFKHGRWLGLANAGLQFITGGRSWGLFDR"
     gene            1554..>1804
                     /locus_tag="AC159P_19195"
     CDS             1554..>1804
                     /locus_tag="AC159P_19195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF255 domain-containing protein"
                     /protein_id="HIR3697620.1"
                     /translation="MSGQRFTNRLAQESSPYLQQHAHNPVDWYPWGEEALQRAKAEDK
                     PILLSIGYSACHWCHVMERESFENEEIAGQMNEQFVNIKV"
BASE COUNT          330 a          602 c          547 g          325 t
ORIGIN      
        1 cgcggtcgaa gagtccccac gaacgcccgc ccgtgatgaa ctgtagcccc gcgttcgcca
       61 gcccgagcca gcggccgtgc ttgaaggccg cgtggaaatt gcgcacgcgc cgcagctcct
      121 gcgtgatcca gctctcgcgt atgagttggc ggtagcgctt gagctgctgc ttcgagtagt
      181 cctcgttgag cagcgcctcg aagatggtct gcgcggcgag catgccgctc ttgatcgcgg
      241 tgtggatgcc cttgaggcgt tgcgagttga ggaagcccgc gcagtcgccc gcgagcagaa
      301 agccgtcgcc gtgaaactgc gggatggcgt agaagccgcc cgccgtaatc gtcttcgcgc
      361 cgtagcggat catcttgccg ccttcgagca gcttcgccac gaacgggtgc tgcttgaagc
      421 gctggaactc cgcgtgcggg tcgaggagcg ggtctcggta atcgaggccc gtcacgtagc
      481 cgaggttcac gatgcggttc tgcattccgt aaatccaccc gccgccgtac gtgctcgcgt
      541 ccgaagggta gccgagcgtg tgcgtcacgc gccccgccgg gtagcggtcg tcgggcagct
      601 cccacagctc tttcacgccg aggctgtaga cctgcggctc gcgcccgtca ttcaggttca
      661 aacgctgcgt gagctgcttg gcgagcgagc cgcgcggccc ctcgcccaag accgtgacct
      721 tcgccagcag atcgacgccc ggctcgtagt tcgcgcgccg ccgcccttcc ctgtccacac
      781 ccttgtcgcc cgtgcggacg ccgacgacgc ggtcttgctc gtcgtacagc atctcggcgc
      841 cggggaactc cgggaagatg ttgacgcccg cctcctcgca cttctcgccg agccacgccg
      901 tcagcttgtt cagcgagacg atgtaatacc cgtggttctt caaaggcggc ggcgtgatcg
      961 gcgacttgat ggcgcgcgtc ttcgtgaggt aaagaaaata atcttccttc accggcgatt
     1021 cgagcggcgc gccctccgac tcgaagtccg ggatcagctc gcgcagcgcc ttcggatcca
     1081 tcaccgcgcc tgacaggatg tgcgcgccga ccgacgcgcc cttctcgatg accgcgatct
     1141 gcaactcgcc gagcggcttg ccctgccgct cgcccttcgc gatcaactcg tcgtgctgct
     1201 tgacgagccg cgccagatgc agcgccccgc tcaggttcgc cgggcctgcc ccgacgaaga
     1261 cgacatccat ctcaaggctc tctcgttcca ttcgcaaact ccaatcgaaa aggacagacc
     1321 attaacacga gtgatgcggg caagaccgcc ccgcgcggcg tgcgcgaaag gtcttaccgg
     1381 caatcacggc gccgaccacg aaaaatgaat caccattcat ttttcagttt agccccgccg
     1441 ccccgaaata gcaaaagtcc gatcgtgcgt tgcgcgcccg acgcgtttgc cgaaactcga
     1501 cggcgaggca cataatcggt tcaaacaatt ttcaggcgaa ggaatcattc gagatgtctg
     1561 gtcaacggtt caccaaccgg ctcgctcagg agagcagccc ctatcttcaa cagcacgcgc
     1621 acaaccccgt ggactggtat ccgtggggcg aagaggcgct tcagcgcgcg aaggccgagg
     1681 acaagccgat cctgttgagc atcggctact cggcctgcca ctggtgccac gtgatggagc
     1741 gcgagtcatt cgagaacgag gagatcgcgg gccagatgaa cgaacagttc gtcaacatca
     1801 aagt
//
LOCUS       DBNGOH010000985         2076 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4180031, whole genome shotgun sequence.
ACCESSION   DBNGOH010000985 DBNGOH010000000
VERSION     DBNGOH010000985.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2076)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2076)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2076
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4180031"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..901
                     /locus_tag="AC159P_19200"
     CDS             <1..901
                     /locus_tag="AC159P_19200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697621.1"
                     /translation="QNVKLYASRTESLSAGGQLRLRPDGGADDGYETLGDMDVVVVNT
                     GENGLFTKGEFQAITHMRQMEFITPEEIARQVVLEVKGSSTGYDVIAAIDGASMNPSY
                     RAGYLRHLAIRELERLEEEKSAPSVALGELGPPELGKLLWEAYLLRVACDNSVKAVLS
                     KSSAEIADLVSRKVEEDERLRNIIVSVGLPILVPGGGDAPSLIRGPYIRIPEEKNKDA
                     ADIRSLADVDAWAAKGWVDLRPGNMELWRRRFDKMRHETERVRERGSAAFTRKAYLTD
                     EIEIGTVVGWVFNNEEDFGYRIK"
     gene            907..>2076
                     /locus_tag="AC159P_19205"
     CDS             907..>2076
                     /locus_tag="AC159P_19205"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF015448.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HIR3697622.1"
                     /translation="MNISYNWLRELAGVALEPSELARRMTMIGLAVDSVEEGGDDFVL
                     DFDLTSNRPDCLSHLGLAREAAALAGGRVNLPALTASAVEGHAEFYTAVEILDPDLCP
                     RYAARVVHGVKIGPSPSWLVERLQAIGQRPINNVADITNYVMHEMGQPLHAFDLATLA
                     ERRIVVRRAHLGEKLKTLDGVERVLDAEMLVIADAARAVALAGVMGGEETEISEATTD
                     VLIESAYFSPASVRRTSKVLGLQTEASYRFERGTDYEGVRRAQDRCVALICDLAGGTA
                     TVDAIDVYPQPINSRSVSLRPRRVKALTGLDVSGDECGRILNALGFETKGEQDAMDAV
                     RDAKDGGLDARTTYAVPSWRVDVALEEDLVEEVARHFGYDKIEDTLPSSGASGEYL"
BASE COUNT          361 a          682 c          728 g          305 t
ORIGIN      
        1 ccagaacgtg aagctctacg ccagccgcac cgaaagcttg agcgcgggcg gccagttgcg
       61 gctgcggccc gacggcggcg cggacgacgg ctacgaaacg ctcggcgaca tggacgtggt
      121 ggtcgtcaac acgggcgaga acggcctctt cacgaagggc gagtttcagg ccatcacgca
      181 catgcggcag atggagttca tcacgcccga agagatcgcg cggcaggtcg tcctcgaagt
      241 gaagggcagc agcacgggct acgacgtgat cgcggccatc gacggcgcgt cgatgaaccc
      301 gagctaccgc gcgggctacc tgcgccacct cgccatcagg gagctggagc ggctcgaaga
      361 agagaagtcg gcgccgtccg tggcgctcgg cgagctgggg ccgcccgagc tgggcaagct
      421 cctgtgggaa gcctacctgc tgcgcgtcgc ctgcgacaac agtgtcaagg ccgtgctctc
      481 gaagtcgagc gcggagatcg ccgacctcgt ctcgcgcaaa gtcgaagagg acgagcggct
      541 gcgcaacatc atcgtctcgg tcgggttgcc gatactcgtc cccggcgggg gcgacgcgcc
      601 gtcgctcatc cgcggtcctt acatacgcat ccccgaggag aagaacaagg acgccgcgga
      661 catccgctcg ctcgcggacg tggacgcgtg ggcggccaag ggctgggtcg atttgcggcc
      721 cggcaacatg gagctttggc ggcggcggtt cgacaagatg cggcacgaga ccgagcgcgt
      781 gcgcgagcgc ggctcggcgg ccttcacgcg caaggcttac ctgaccgacg agatagagat
      841 cggcaccgtc gtcggctggg tcttcaacaa cgaagaggac ttcggatacc ggatcaaata
      901 ggcgttatga acatcagtta caactggctg agggaactcg cgggcgtggc gctcgaaccg
      961 tcggagctgg cgcggcggat gacgatgatc ggactggcgg tggactcggt cgaagagggg
     1021 ggcgacgact tcgtcctcga tttcgacctg acctcgaacc ggcccgactg cctctcgcac
     1081 ctcggcctcg cgcgcgaggc ggcggcgctc gccggcggtc gcgtcaacct gcccgcgctc
     1141 acggcctccg cggtcgaagg ccacgccgaa ttctacacgg cggtcgaaat cctcgacccc
     1201 gacctctgcc cgcgctacgc ggcgcgcgtc gtgcacggcg tgaagatcgg gccgtcgccg
     1261 tcgtggctcg tcgagcggct gcaagccatc gggcagcgcc ctatcaacaa cgtcgccgac
     1321 atcacgaact acgtcatgca cgagatgggc cagccgcttc acgccttcga cctcgccaca
     1381 ctcgccgagc gccgcatcgt cgtgcgccgc gcgcatctgg gcgagaagct gaagacgctc
     1441 gacggcgtgg agcgcgtgct cgacgccgag atgctggtca tcgcggacgc cgcgcgcgcc
     1501 gtcgcgctgg cgggcgtgat gggcggcgag gagacggaga tttccgaggc gacgaccgac
     1561 gtgctcatcg agagcgcgta cttcagcccg gcgtccgtgc gccgcacgtc gaaggtgctc
     1621 ggcctccaga ccgaagcctc ctaccgcttc gagcgcggga cggactacga gggcgtgcgc
     1681 cgcgcgcagg atcgctgcgt cgcgctcatc tgcgacctcg cgggcggcac cgccaccgtt
     1741 gacgccatcg acgtttaccc gcaacccatc aattcgcgct ccgttagctt acgcccgcgc
     1801 cgcgtcaagg cgctgaccgg cctcgacgtg tcgggggacg aatgcgggcg catcctgaac
     1861 gcgctcggct tcgagacgaa gggcgaacag gatgcgatgg acgccgttcg tgacgcgaag
     1921 gacggcggac tcgacgcgcg gacgacttac gcggtgccgt cttggcgcgt ggacgtggcg
     1981 ctggaggaag atttggtcga agaggtcgcg cgccacttcg gctacgacaa gatcgaggac
     2041 acgctgcctt cttcgggcgc gtcaggcgag tatctg
//
LOCUS       DBNGOH010000986         2755 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3630714, whole genome shotgun sequence.
ACCESSION   DBNGOH010000986 DBNGOH010000000
VERSION     DBNGOH010000986.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2755)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2755)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2755
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3630714"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..807)
                     /locus_tag="AC159P_19210"
     CDS             complement(<1..807)
                     /locus_tag="AC159P_19210"
                     /inference="COORDINATES: protein motif:HMM:NF028473.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HIR3697623.1"
                     /translation="MKKRNPVLAATLITLTLAVGQSTALASMSLSFQRTWGGLNDEHA
                     EGVAVAPDGSVYVAGGTNSFGTGAGVGDQDAFLLKYTPGGSIAWQKTYGAGRSDPLIV
                     ADDVATDVAVGADGAVYVAGFSTAGHAFLVRFDPAGNLLWQRAWGGDGFELPDAVAVA
                     ADGSLYIAGFTNSFGAGDQDAFLVKYTPDGSVVWERTWGGAGLDAARDVAVAGDGSVY
                     IAGDTNSFFANDAFLVKYDSAGAIVWERAWREGTIEDISGALGVATAPDGS"
     gene            1120..1881
                     /locus_tag="AC159P_19215"
     CDS             1120..1881
                     /locus_tag="AC159P_19215"
                     /inference="COORDINATES: protein motif:HMM:NF025887.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4440 domain-containing protein"
                     /protein_id="HIR3697624.1"
                     /translation="MRRIISLLSLSLLASLTALAQDAATADAQHYKAEFENDKVRVLR
                     VKIGPRGKTAMHSHPDAVVIAVTDTKVRHHSPDGKSQEAEFKAGAAHWTPAATHMGEN
                     LTDKPLEVILVELKSSASASASRAGDATAALMQLEREWADAVKRADTAWMEQHYAPEY
                     TWTSPEGTVNDRKTDIEDAKNYSFDSLEVSDTRVRVIGDTAVVTGVTALKGKYKGQDF
                     SGSYRFTDTLVRRDGRWLILASQYTRVAAQQASAK"
     gene            2388..>2755
                     /locus_tag="AC159P_19220"
     CDS             2388..>2755
                     /locus_tag="AC159P_19220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697625.1"
                     /translation="MERRNDFSPDEDRDETLVTPRFDSDEAETARPVVPLAVVSGEAD
                     TYDPPAARRAPRVMSRRSWPLALILVSALAGSVLGGASLYLFQKSRQDGAAAAPVEQT
                     TAAATEQAVAPAPETTPEPSP"
BASE COUNT          489 a          931 c          896 g          439 t
ORIGIN      
        1 gctgccgtcc ggggcggtcg ccacgccgag cgcgccgctg atgtcttcga tggtgccttc
       61 gcgccaggcc ctctcccaga cgatggcccc cgcggagtcg tacttcacga ggaaggcgtc
      121 gttggcgaag aagctgttcg tgtcccctgc gatgtagacg ctgccgtcgc ccgccacggc
      181 cacgtcacgc gcggcgtcca ggcccgcccc gccccacgtc ctctcccaaa ccaccgagcc
      241 gtcgggcgtg tatttgacga ggaaggcgtc ctgatcgccc gcgccgaagc tgttcgtgaa
      301 cccggcaatg tagaggctgc cgtccgcggc gacggccacg gcatccggca actcgaaacc
      361 gtcgccgccc caggcacgct gccagaggag gttgccggcg gggtcgaacc tgacgaggaa
      421 cgcatgtccg gcggtggaga accccgcgac gtagaccgcc ccgtcggctc cgaccgcgac
      481 atccgtggcg acatcgtcgg cgacgatgag gggatcgctc cgccccgccc cgtatgtttt
      541 ctgccacgcg atgctcccgc ccggcgtgta cttcaacagg aaggcgtctt ggtcgcccac
      601 ccccgcgccg gtgccgaagc tgttcgtgcc gcccgcgacg tagacgctcc cgtccggggc
      661 gacggcgacc ccttcggcgt gttcgtcgtt caagcccccc cacgttcgtt ggaacgagag
      721 gctcattgac gccaacgcgg tcgattgccc cacggccagc gtcagtgtga tgagcgtcgc
      781 ggccaggacg gggtttctct ttttcatacg ttcacctcgc gaagtgttga ataaccgatg
      841 acaagtaagg ggtcgtcatt ggggcgcgtc ggggcggacg ctgcctttag gagcgcgtcc
      901 atgaacctca ccttcaacag tgtctgccgc gcgccgttaa atacacaggg agtggtcggc
      961 tacgcgcgcg gcggcggagt cgtggctcgg acgacgtatt gtcggtttcg cccttcggtg
     1021 atagagtgcc gcccgcaaca gggggcacac gggcgcgaac ctcccaccgc cgacgcctga
     1081 cgactccgca accaccgacc accaacttag gaggacacca tgagacgcat catctcccta
     1141 ctctctcttt ccctactcgc ttcactcact gccctcgcgc aagacgcggc cacggcagac
     1201 gcgcagcact acaaggcgga gttcgagaac gacaaggtac gcgtcctgcg cgtgaagata
     1261 ggcccccgcg ggaagaccgc catgcactcg cacccggacg ccgtcgtcat cgccgtcacc
     1321 gacacgaagg tgcgccacca ctcccccgac ggcaaatcgc aggaggcgga gttcaaggcc
     1381 ggggccgcac actggacgcc ggcggcgacc cacatggggg agaacctgac cgacaagccg
     1441 ctggaggtca ttctggtgga gctgaagtcc tcggcctcgg cttcggcctc ccgagccggt
     1501 gacgccacgg cggcgctcat gcagttggag cgcgagtggg cggacgccgt gaagcgcgcg
     1561 gacacggcgt ggatggaaca gcactacgcg cccgagtaca cttggacgag tcccgaaggc
     1621 accgtcaacg acaggaagac ggacatcgag gacgcgaaga actacagctt cgattcgctg
     1681 gaggtgtcgg atacgcgtgt gcgggtcatc ggcgatacag ccgtcgtcac cggcgtcacg
     1741 gcgctcaagg gcaagtacaa gggccaggac ttcagcggca gttatcggtt caccgacacg
     1801 ctcgtgcgga gggacgggcg gtggctgatt ctggcctcgc agtacacgcg cgtcgccgcc
     1861 cagcaggcga gcgcgaaata gaccgacagc gatcccacgg cttcaaggga agcgaacggg
     1921 cgcgacgaaa ccagcaggca gtaggcagcg ggcagtaggc agccaaagca agcagactgg
     1981 agtcgcagtc aggagttaga agaagcagga ggcttgagac gtggcgtctc aagcctcctg
     2041 cttcattgtt cgcagagcga agaggctcgg gactttacct cccaagctgc tccggtctgc
     2101 ttccgtcttg actgcctact gcccgctgcc taccgcctac tttctcggag tgtctggttt
     2161 gcaaaccgca tgtcgttttt agggcgcacg atatcggttg aggtcgccgc ccggacattt
     2221 ctcccaccgt ccgatctcgc cgccggagag cgccgtaact gcaactaatt tgtaatttaa
     2281 aggggacagc ggcgccgggc ggggccgcat ctcactggcg aggtttttcc ggcctcgggt
     2341 ttgcagtcaa atctcgtgca gacggttcga gggccgggcc agtgaagatg gaaagaagga
     2401 acgatttttc tccggacgaa gaccgggacg agactctggt cacgccgcgc ttcgacagcg
     2461 acgaggcgga gacggcgcgg cccgtcgtgc cgctcgccgt cgtgtcgggc gaggccgaca
     2521 cctacgaccc gcccgccgcg cgccgcgcgc cgcgcgtcat gtctcgcagg tcttggccgc
     2581 tcgcgctcat cctcgtctcc gcgctcgcgg gcagcgtgct cgggggcgcc agcctctatc
     2641 tcttccagaa gagtcggcag gacggagccg ccgcggcgcc ggtcgagcag acgaccgccg
     2701 ccgccacgga gcaggcggtc gcgcccgctc cagagacgac ccccgaacct tcgcc
//
LOCUS       DBNGOH010000987         3003 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2532989, whole genome shotgun sequence.
ACCESSION   DBNGOH010000987 DBNGOH010000000
VERSION     DBNGOH010000987.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3003)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3003)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3003
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2532989"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..119)
                     /locus_tag="AC159P_19225"
     CDS             complement(<1..119)
                     /locus_tag="AC159P_19225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde 3-phosphate dehydrogenase
                     NAD-binding domain-containing protein"
                     /protein_id="HIR3697626.1"
                     /translation="MAIKVGINGFGRIGRNVLRASMGDADIDFVAVNDITDTKT"
     gene            633..2915
                     /locus_tag="AC159P_19230"
     CDS             633..2915
                     /locus_tag="AC159P_19230"
                     /inference="COORDINATES: protein motif:HMM:NF014385.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HIR3697627.1"
                     /translation="MSRRYSFAAESRRIAFFLAAFSLAANFAFAGTAQALASGRSGGQ
                     KSKAAPARAKKVKPAAAEPTLVSENTAPQPSQRPAAANAFKTVGEPFIALTAQTQQQQ
                     TPAQRDATRPPGTEQRQTVPPDARPRPSDPTAPPGTERQQSPRTPPGTTAPSQTQDRR
                     TPVPAPTQDPTAPPVSTDPLETPTPEAPADQQLAEPDFPAVQPRPVPPLPSLARVGVN
                     RDDTLPLTLNEAIRRALENNNTIEVARNDVRIAEQTLLGLRGVFDPSIVYRSEVNSSI
                     RPVPNIFGGAGSSGTVSTTDYNNDVSVTKQFGKGGGNYTYFFNNTRENTDANNTTLNP
                     FYSSNQGITFTQPLWRNRSIDRNRQQIRIQQKRLQQSDADFRRNTIEVISQVQRAYWD
                     LVFALRDEQNQIANLNLARENFRRTEASVAAGASAPLERAEIQTELSTREQSLLIASQ
                     NVTVAENNLKNLMLKDPLNPEWSRALLPTDQPSFDDTPLSLEASLKEARANRPEMRRL
                     QLQQEISDIDLQFFKNQTKPQIDLTGTLSTTGLAGTQNITTDTTPRELIDIADTTDPA
                     AFLLRQLNDTRAALPTPLPPINVPTITPSAGTLPSQFLGGYGRTLRNLFSFDTRNVVV
                     GVTIQFPLKNRTAEANLASAHIAREQLAAQTRSQDQVIEVEVRNSVQAVETARRRVLA
                     ARQARQSAEEQLAGERRLYQVGRSTTFLLFQRENQLVNARNQELRAETDYNKALADLQ
                     RATSTTLRVNNVVIESPYVP"
BASE COUNT          558 a         1158 c          867 g          420 t
ORIGIN      
        1 gtcttggtat ccgtgatgtc gttgacggcc acgaagtcga tgtcggcgtc gcccatgctc
       61 gcgcggagca cgttgcgacc gatgcgcccg aacccgttaa tcccaacttt tattgccatc
      121 cgatgtaacc tcctcgacgt gcgtgtcgtc tatcttattt tgagcgtgcg cgtgattaga
      181 gcgcgcgcat ccgccgcccg taaagtgcca tttcgggtcg gacgtggggg ccacgcggcg
      241 ctccgcggcg aagcgggagc gcccgcgcct tctggaattg gaaaacttgc tcaagtaaac
      301 tgcaaataat gctcatgcga ctgcgcactg tcaagtcagc cggggggcgc aggcgtaaaa
      361 gtgccgccgc tctctcgccc ctgtttcgga tgcgcgccgc gcgcgttggg cggcgatggc
      421 ggcgtggcct aaatccatgt ttttgaaacc acctcgccgc gcgcgacgta gtaggctctg
      481 attcacggcg actgctctgg aagggatttt ggcgggccat tgccgttcaa tttcggtttt
      541 caacagacga catttcaaac acgtaacaca gcgccggagc gccgccatct cttcccgcgg
      601 cgcgacggcc cggaagatca aggaaagcaa cgatgtccag acgatacagt ttcgccgccg
      661 agtcgagaag aatcgccttc ttcctcgccg ccttcagcct cgccgccaac ttcgccttcg
      721 cgggcacggc gcaggcgttg gcctcgggca ggtcgggcgg ccagaagtcg aaggcggccc
      781 cggcccgcgc caagaaggtc aagcccgccg cggcggagcc gaccctcgtc agcgagaaca
      841 ccgccccgca gccttcgcag cggcccgccg ccgcgaacgc gttcaagacc gtgggcgagc
      901 cgttcatcgc cctgacggcg cagacgcagc agcagcagac gcccgcgcag cgcgacgcca
      961 cgcgcccgcc cggcaccgag cagcggcaga ccgtcccgcc cgacgcgcgc ccgcgcccct
     1021 ccgacccgac cgcgcctccg ggcaccgagc gccagcagtc gccgcgcacg ccgcccggca
     1081 cgaccgcccc ttcgcagacg caggatcgcc ggacgcccgt cccagcgccg acgcaagacc
     1141 cgaccgcgcc gcctgtcagc accgacccgc tggaaacgcc gacgcccgag gcgcccgccg
     1201 accagcagct cgccgagccg gacttccccg ccgtccagcc gcgccccgtg ccgccgctgc
     1261 cctcgctggc gcgcgtcggc gtcaaccgcg acgacacgct cccgctgacg ctgaacgagg
     1321 ccatccgccg cgcgctcgaa aacaacaaca ccatcgaggt ggcgcgcaac gacgtgcgca
     1381 tcgccgaaca gacgctgctg ggcttgcgcg gcgtcttcga ccccagcatc gtctaccgct
     1441 ccgaggtcaa cagctcgatc cgcccggtgc cgaacatctt cggcggcgcg ggttcgtcgg
     1501 gcaccgtctc gaccaccgac tacaacaacg acgtctccgt caccaagcag ttcggcaagg
     1561 gcggcggcaa ctacacttac ttcttcaaca acacgcgcga gaacaccgac gccaacaaca
     1621 cgacgctcaa cccgttctac tcttcgaacc agggcatcac tttcacgcag ccgctctggc
     1681 gcaaccgctc catcgaccgc aaccgccagc agatccgcat ccagcagaag cgcctccagc
     1741 agtcggacgc cgacttccgc cgcaacacca tcgaggtcat ctcgcaggtg cagcgcgcct
     1801 actgggattt ggtcttcgcg ctgcgcgacg agcagaacca gatcgccaac ctcaacctcg
     1861 cgcgcgagaa cttccgccgc accgaggcga gcgtcgccgc gggcgcgtcc gccccgctcg
     1921 aacgcgccga gattcagacc gagctttcga cgcgcgagca gtcgctgctc atcgcctcgc
     1981 agaacgtcac ggtcgccgag aacaacctca agaacctgat gctcaaagac ccgctcaacc
     2041 ccgagtggtc gcgcgcgctt ctgccgaccg accagccgag cttcgacgac acgcccctca
     2101 gcctcgaagc ctcgctcaag gaggcgcgcg ccaaccgccc cgagatgcgg cggctgcaac
     2161 tccagcagga gatcagcgac atcgacctcc agttcttcaa gaaccagacc aagccgcaga
     2221 tcgacctgac cggcacgctc tcgaccaccg gcctcgccgg cacgcagaac atcacgaccg
     2281 acacgacgcc ccgcgaactg atcgacatcg ccgacacgac cgacccggcg gccttcctgc
     2341 tccggcagct caacgacacg cgcgcggcgc tcccgacgcc gctgcccccg atcaacgtcc
     2401 cgaccatcac gccgtccgcc ggcacgctcc cgtcgcagtt cctcggcggc tacggcagga
     2461 cgctccgcaa cctcttcagc ttcgacacgc gcaacgtcgt cgtcggcgtg accatccagt
     2521 tcccgctcaa gaaccgcacc gccgaagcga acctcgcctc ggcccacatc gcgcgcgagc
     2581 agctcgccgc gcagacgcgc tcgcaggatc aggtcatcga ggtcgaagtc cgcaactccg
     2641 tgcaggcggt cgagacggcc cgccgccgcg tgctcgccgc gcggcaggcc cgccagagcg
     2701 ccgaggagca gctcgccggc gagcgccgcc tctatcaggt cggtcgctcc accacgttcc
     2761 tcctcttcca gcgcgagaac cagctcgtca acgcgcgcaa ccaggagctg cgcgccgaga
     2821 ccgactacaa caaggcgctc gccgacctgc aacgcgccac gtccaccacg ctgcgcgtca
     2881 acaacgtcgt catcgagtcg ccttacgtac cgtaagtagc ctgtgatgcg acttcggatt
     2941 taacgcagag tgcgcggaga tgcgcagagg gcgcagagaa aacattcgtc ttctctgcgc
     3001 cct
//
LOCUS       DBNGOH010000988         1908 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_516101, whole genome shotgun sequence.
ACCESSION   DBNGOH010000988 DBNGOH010000000
VERSION     DBNGOH010000988.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1908)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1908)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1908
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_516101"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1840)
                     /locus_tag="AC159P_19235"
     CDS             complement(<1..1840)
                     /locus_tag="AC159P_19235"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3697628.1"
                     /translation="MRIADSIESADYTSTRREEGIANPQSAIRNPQSRVLLADDNADM
                     REYLRRLLAESGYEVEAVADGLAALQAARERPPELLLTDVMMPGLDGFGLLAALRAEE
                     RTRTLPVILLSARAGEEARVEGMEAGADDYLTKPFSARELLARIESHVRLHRVRLEAE
                     EALLRSREQLQLVSDNVPALISYVDSERRYRWCNRAYTIWFGLPTEQVVGREMRHVLG
                     EEAWQTIGPRVEAALGGEPVEYEAEAKYKTGGTRWIHAVYNPHRDDKGEVRGVVVMVR
                     DVTERKRAEEERDRLLAREQAARSQAEAANRLKDDFLATVSHELRTPLTAMLGWAHML
                     RAGGLDEAGRARALDTIERNARAQQQLVEDLLDVSRIITGKLRLDVRPCDPASFIEAA
                     VEAVRPAAEAKEVRLQKVLDAGMTSISGDPARLQQVVWNLLANAIKFTPRGGRVQVRL
                     ERVNSHVEIAVSDTGAGIAPEFLPHVFERFRQADSSTTRAHGGLGLGLAIVRHLVELH
                     GGTVHADSHGEGLGATFTVKLPLIAVYAREPAGERVHPRASDQPLLPVECPERLDGLK
                     VLVVDDERDAGELLRVMLARCGAEITTALSAEEALAEMRRTRFDLLV"
BASE COUNT          277 a          687 c          633 g          311 t
ORIGIN      
        1 agacgagcag gtcgaaacgc gtccgccgca tctcggcgag cgcctcttcc gccgaaaggg
       61 cggtcgtgat ttcggcaccg caccgcgcga gcatcacgcg cagcaactcg cccgcgtccc
      121 gttcgtcatc gaccaccaac accttcaagc cgtcgagccg ctccgggcat tccaccggca
      181 gcagcggctg gtcgctcgcg cgcgggtgga cgcgctcccc cgccggctcg cgggcgtaga
      241 cggcgatgag cggcagtttc acggtgaagg tcgcgcccag cccttcgccg tgactgtcgg
      301 cgtgaaccgt gccgccgtgc aactcgacca gatggcgcac gatggcgaga cccagaccca
      361 gcccgccgtg cgcccgcgtg gtcgagctgt cggcctgccg gaagcgctcg aagacgtgcg
      421 gcaaaaactc gggcgcgatg ccggcgcccg tgtcgctgac ggcgatttcg acgtgcgagt
      481 tgacgcgctc caatctcacc tgcacgcgcc cgcctctggg ggtgaacttg atcgcgttgg
      541 cgagcaggtt ccagaccacc tgctgcaagc gcgccgggtc gccggagatc gaagtcatgc
      601 cggcgtcgag cactttctgg aggcgcacct ctttggcctc cgccgcgggc ctgaccgcct
      661 cgaccgccgc ctcgatgaac gaggccgggt cgcagggccg cacgtcgagc cggagcttgc
      721 ccgtgatgat gcgcgacacg tcgagcaggt cttcgacgag ctgctgctgc gcgcgggcgt
      781 tgcgctcgat ggtgtcgagc gcgcgcgccc gcccggcttc gtcgaggccg ccggcgcgca
      841 gcatgtgcgc ccagccgagc atcgccgtca gaggcgtccg cagctcgtgc gagaccgtgg
      901 cgagaaaatc gtccttgaga cggttcgccg cctccgcctg tgagcgcgcg gcctgttcgc
      961 gcgcgagaag gcggtcgcgc tcctcttcgg cgcgtttgcg ttcggtgacg tctctgacca
     1021 tcaccacgac gccccgcact tcgcctttgt cgtcgcgatg agggttgtag acggcgtgaa
     1081 tccagcgcgt gcccccggtc ttgtatttcg cctccgcctc gtactcgacg ggttcgccgc
     1141 cgagggcggc ctcgacgcgc gggccgatgg tctgccacgc ttcctcgccg agcacatggc
     1201 gcatctcgcg accgacgacc tgttcggtgg gcaggccgaa ccagatcgtg taggcgcggt
     1261 tgcaccagcg gtagcggcgt tcgctatcga cgtaggaaat cagcgcgggg acattgtcgc
     1321 tgaccagttg tagctgctcg cggctgcgca gcagggcttc ttccgcttcg aggcgcacgc
     1381 gatgcagccg cacgtgcgac tcgatgcggg ccagcagctc gcgcgccgaa aacggcttgg
     1441 tcaggtaatc gtcggccccg gcttccatcc cctcgacccg cgcctcctcg cccgcgcgcg
     1501 ccgagagcag gatgacgggc agcgtcctcg tccgctcctc cgcgcgcagg gcggcgagca
     1561 gaccgaagcc gtcgaggccc ggcatcatca cgtccgtcag cagcagctcg ggcgggcgct
     1621 cgcgggccgc ctgcaaagcc gccagaccgt cggccacggc ctcgacttcg taaccgctct
     1681 ccgcgagcag ccgccgcaga tactcgcgca tgtccgcgtt gtcgtcggcg agcagaaccc
     1741 tcgattgcgg attgcggatt gcggattgcg gattggcgat cccctcctcc cgccgcgttg
     1801 acgtgtagtc cgccgactcg atcgaatccg caatccgcaa tccgtaatcc gtattggccg
     1861 ggaaccagcg caatacctct tcgacgtaag cctcgccccg cagtccgg
//
LOCUS       DBNGOH010000989         3263 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_149136, whole genome shotgun sequence.
ACCESSION   DBNGOH010000989 DBNGOH010000000
VERSION     DBNGOH010000989.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3263)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3263)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3263
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_149136"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            223..2547
                     /locus_tag="AC159P_19240"
     CDS             223..2547
                     /locus_tag="AC159P_19240"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF042649.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HIR3697629.1"
                     /translation="MQQQQQQPPAKQKRKVRVPRVVNGVETMVEIEVEEEVGGAVWGA
                     NDKHTLLNSRLTRVDGPLKVTGAAEYTYDKRLPGMLHGRVLRSPHAHARVIRVDASAA
                     RRIPGVRAIVGATDEAVTEGANRAAAEADRQNPTEVGGARPAKKEKIVRFAGEPVAAV
                     AADTPEIAGDAIRAIRVEYEVLPHVVRADDALKPGAPQVYPEGNLEPKDKRGDAAKVA
                     AALAAAEVVYEAEYRTPIIHHASLETHGNVVDYRGGDAATVYASTQGTFTIPGDAAKE
                     LDLKQSAVTAIVEHMGGGFGSKFGIGIEGMFACQLAKQAKAPVKLMLTRADEFVLAGN
                     RSGSWQKFKAGATRDGKLVALHATQYQIGGLGDGSQAGQPYIYTVPEAYRERIAIHTH
                     EDSSRAMRAPGHPQASYAIECLIDDLANKIGLDPVEFRKKNLKDTVYHRQLDRGAREI
                     GWERRNKTPGAGAGPLKRGLGCAVGTWGGGGNNQCKVDVTIARDGAVVVAVGTQDLGT
                     GTRTYTRAIVAEEFGLGMKDVSERIGDSRLGQANSSGGSTTAASLAPSVKDAAHKARL
                     AVAEKVSPLLGGAKPEEIKFERGTVSGGGKSLAWKQACAALPAAGVTARGEWKSSLSA
                     SGVHGVCFAEVEVDVETGHVRAVKMVHVQDIGLPLNRLALESQINGGMIQSLGMTLYE
                     GRVMDAQLGVQLNPGFGDYKLPGSLEMPELVPLIDDEDKREAVIGVGEPAIIPSVGAL
                     ANAVFNACGVRVAELPLTPDKILMALAGVGRRSA"
     gene            2544..>3263
                     /locus_tag="AC159P_19245"
     CDS             2544..>3263
                     /locus_tag="AC159P_19245"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013134.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HIR3697630.1"
                     /translation="MKAFEWVSPTNIDEAVKLLKDAPAGGDADEAARPIAGGQDLLTT
                     MKEYITRPTRVVNLKNIGGLDRIRSDGKKGLRIGALVTLTQLEESAEVRRAFPGLAEA
                     AHSIATPQIRNLGTVGGNLCQRPRCWYYRLEEVVCLKKGGSECYAAKGENKYNAIFGG
                     GPSYIVHPSDLAPVLVALGASVNVTGASGKRTIPLDKFFTLPNEGSIRRENVLKNDEI
                     ITEVVIPASGFAARSTYLKFKE"
BASE COUNT          598 a         1065 c         1152 g          448 t
ORIGIN      
        1 tgcaaaggca acttctgccg ctgcggcacc tacccgcgcg tcttcgaggc cgcgctcgcc
       61 gccgcgaaga ataacaataa gtctggagtc tgaagtctgg agtctggagt cagagcaaag
      121 atttttcttc tctcctgacc ccggacttca aacgcaggac gttgctttaa aaattctgac
      181 tccagacttc ggactccaga ctttctttca ccggagagag ccatgcagca gcaacaacag
      241 cagccgcccg cgaagcagaa gaggaaggtg cgggtgccgc gcgtcgtgaa cggcgtggag
      301 acgatggtcg agatcgaggt cgaggaagag gtgggcggcg cggtgtgggg tgcgaacgac
      361 aagcacacgc tcctgaacag ccggctgacg cgcgtggacg ggccgctgaa ggtgacgggc
      421 gccgccgagt acacctacga caagaggctg ccgggcatgc tccacgggcg cgtgctccgt
      481 tcgccgcacg cgcacgcgcg cgtcatcagg gttgacgcgt ccgccgcgcg gcgcatcccc
      541 ggcgtgcgcg ccatcgtcgg cgcgacggac gaggcggtga ccgaaggcgc caacagggcc
      601 gccgccgagg ccgaccggca gaacccgacg gaggtgggcg gcgcgcggcc cgcgaagaag
      661 gaaaagatcg tgcgcttcgc gggcgagccg gtcgcggcgg tcgcggcgga cacgcccgag
      721 atcgcgggcg acgccatccg cgccatccgc gtcgagtacg aagtgctgcc gcacgtcgtc
      781 cgcgccgacg atgcgctgaa gccgggcgcg ccgcaggtct atcccgaagg caaccttgag
      841 ccgaaggaca agcgcggcga tgcggcgaag gtcgcggcgg cgctcgcggc agccgaagtg
      901 gtttacgagg cggagtaccg cacgccgatc atccatcacg cgtcgctcga aacgcacggc
      961 aacgtggttg actatcgcgg cggcgacgcg gcgaccgtct acgcctcgac gcaaggcacc
     1021 ttcaccatcc ccggcgacgc ggccaaagaa ctcgacctca agcagagcgc cgtcaccgcc
     1081 atcgtcgaac acatgggcgg cggcttcggg tcgaagttcg gcatcggcat cgaagggatg
     1141 ttcgcctgcc aactggcgaa gcaggcgaag gcgccggtca agctgatgtt gacgcgcgcg
     1201 gacgagttcg tgctcgcggg caaccgctcc ggctcgtggc agaagttcaa ggcgggcgcg
     1261 acgcgcgacg gcaagctcgt cgccctgcac gcgacgcagt accagatcgg cggcctcggc
     1321 gacggctcgc aggcgggcca gccatacatc tacacggtgc cggaagccta ccgcgagcgc
     1381 atcgccatcc acacgcacga agactcttcg cgcgcgatgc gtgcgcccgg ccacccgcag
     1441 gcgagctacg ccatcgagtg tctgatcgac gacctcgcga acaagatcgg cctcgacccg
     1501 gtcgagttcc gcaagaagaa tttgaaggac acggtgtacc accgtcagct cgaccgcggc
     1561 gccagagaga tcggctggga gcggcgcaac aagacgccgg gcgcgggggc ggggccgctc
     1621 aagcgcgggc tggggtgcgc ggtcgggacg tggggcggcg gcggcaacaa ccagtgcaag
     1681 gtcgatgtca ccatcgcgcg cgacggcgcg gtcgtggtcg ccgtcggcac gcaggatttg
     1741 gggacgggca cgcgcaccta cacgcgcgcc atcgtcgccg aagagttcgg gctggggatg
     1801 aaagatgtct ccgagcgcat cggcgactcg cggctcgggc aggccaactc ttcgggcggc
     1861 tcgaccacgg cggcttcgct cgcgccgtcg gtcaaggacg cggcgcacaa ggcgcggctc
     1921 gccgtggccg agaaggtctc gccgctgctc ggcggcgcga agccggaaga gatcaagttc
     1981 gagcgcggga ccgtgagcgg cggcggaaag tcgctcgcgt ggaaacaggc ttgcgcggcg
     2041 ctgcctgcgg cgggcgtcac ggcgcgcggc gagtggaagt cgagcctctc ggcgagcggc
     2101 gtccacggcg tctgcttcgc ggaggtcgag gtcgatgtcg agaccgggca tgtgcgggcc
     2161 gtcaagatgg ttcacgtgca ggacatcggc ctgccgctca accggctcgc gcttgagagc
     2221 cagatcaacg gcggcatgat ccagtcgctc ggcatgacgc tctacgaagg gcgcgtgatg
     2281 gacgcgcagt tgggcgtgca gctcaacccc ggcttcggcg actacaagct gcccggctct
     2341 ctggagatgc cggagctggt gccgctcatc gacgacgagg acaagcgcga ggccgtcatc
     2401 ggcgtcggcg agccggccat cattccgtcg gtcggcgcgc tggccaacgc cgtcttcaac
     2461 gcctgcggcg tgcgcgtcgc ggagcttccg ctcacgcccg acaagattct gatggcgctc
     2521 gcgggcgtcg gaaggaggag cgcatgaaag cattcgagtg ggtcagcccg accaatatcg
     2581 acgaggcggt gaagctgttg aaggacgcgc ccgcgggcgg cgacgcggac gaggcggcgc
     2641 ggcccatcgc gggcgggcag gacttgttga cgacgatgaa ggagtacatc acgcggccca
     2701 cgcgcgtcgt caacctgaag aacatcggcg gcctcgaccg catccggtcg gacggcaaga
     2761 agggcctgcg catcggcgcg ctcgtcacgc tcacgcagct cgaagagagc gcagaggtgc
     2821 gacgagcttt ccccggactc gcggaggccg cgcactccat cgccacgccg cagatacgca
     2881 acctcggcac cgtcggcggc aacctctgcc agcgcccgcg ctgctggtac taccgtctgg
     2941 aagaggtcgt ctgcctcaag aagggcggct ccgagtgcta cgccgcgaag ggcgagaaca
     3001 agtacaacgc catcttcggc ggcggccctt cgtacatcgt tcatccaagc gacctcgcgc
     3061 ccgtgctcgt cgcgctcggc gcgagcgtga acgtcacggg cgcttcgggc aagcgcacga
     3121 tcccgctcga caagttcttc acgctcccca acgaggggag catccgccgc gagaacgttc
     3181 tgaagaacga cgagatcatc actgaagtcg ttatcccggc ttccggcttt gcggcgcgct
     3241 cgacctatct aaaattcaag gag
//
LOCUS       DBNGOH010000990         3325 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1800450, whole genome shotgun sequence.
ACCESSION   DBNGOH010000990 DBNGOH010000000
VERSION     DBNGOH010000990.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3325)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3325)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3325
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1800450"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..931)
                     /gene="rsmH"
                     /locus_tag="AC159P_19250"
     CDS             complement(<1..931)
                     /gene="rsmH"
                     /locus_tag="AC159P_19250"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012302536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000154 - rRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HIR3697631.1"
                     /translation="MAESGGGPGTPHRPVLLLETVRLLAPERGGLFVDATLGLGGHSE
                     AILEASEDARVLGIDRDPEALRLAGERLARFGPRFRAVHAKHEEIARVVREAGEREAS
                     GVLADLGVSSLQFDTAERGFSFRHDAPLDMRMDASGQEETAAELLERLPEEEIARVIY
                     EYGEERKSRRIARWIVERREQGKPLKRTGELAELVARAVGHKRGERIHPATRTFQALR
                     IAVNRELEGLGAFVETAIDLLQMGGRLAVISFHSLEDRVVKRTLRRLAGQCECDPRLP
                     RCECGARRAVEVLTKRPVVPTEEEAEANPRARSA"
     gene            complement(1069..1509)
                     /locus_tag="AC159P_19255"
     CDS             complement(1069..1509)
                     /locus_tag="AC159P_19255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HIR3697632.1"
                     /translation="MLRGNYTARMDSKGRLKVPTAFGRYIKDKYGAEVYVTSLTGDCA
                     RIYPLPEWESIEQRLNLVPTMDPARRKFLDRTNYYGQQAEMDAQGRILIHPLLRKAAG
                     VVGDVAVLGYLTYVEVWELERFEQRLLSDPYTEEDEAAMARLGI"
     gene            1810..2592
                     /locus_tag="AC159P_19260"
     CDS             1810..2592
                     /locus_tag="AC159P_19260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HIR3697633.1"
                     /translation="MNFGETLFFGALVALANVLGGLILARPGAPVRKTQLLRYLVALA
                     AGFMLAAIFIEIVPESVEIWAGQEGGAAQGTLSAMMLLLGGYLLIHFFEHTIAPHFHF
                     GAETHPEAVVRPSAAYAAIGGLSIHTFFDGASVAAAFAVDRKVGLLVFLAVLLHKVPE
                     GFTAASIMLASGRTTRQAFRATLFIGAATMVGVLSVALLQTQVKEAVVYALPFSAGVT
                     LYVAASDLIPEVNHLEEKNPLVSLVVFVGAALFYALHLLIEG"
     gene            2677..>3325
                     /locus_tag="AC159P_19265"
     CDS             2677..>3325
                     /locus_tag="AC159P_19265"
                     /inference="COORDINATES: protein motif:HMM:NF016424.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HIR3697634.1"
                     /translation="MITRSRRTWFSTAVLAAVCLLACANAARAQEAPRAFSQSPLPAP
                     TGFVNDFANVIDEATQARMERVLTSLRDKAQIEFAVVTVPTTGGQDIFNYSLAVARGW
                     KIGAPEGEKKGLLLLVAVEDRKYQIQVSRHLEGDLPDGLVGEVGRRMRDPFRAGDYSK
                     GLMDAVQTFIATIGKQRGFTFEEVDQSYAYQQTQRTRPTGTRRTRQGGLGLGACCL"
BASE COUNT          530 a         1114 c         1061 g          620 t
ORIGIN      
        1 tggcgctgcg cgcgcgcggg ttggcttccg cctcttcctc ggtcggcacg acgggccgct
       61 tcgtcagcac ctcgacggcg cggcgcgcgc cgcactcgca acgcggcaga cgcgggtcgc
      121 attcgcactg cccggccagt cgccgcagcg tccgcttcac cacgcggtct tccaaagagt
      181 ggaagctgat gaccgcgagc cgcccgccca tctgtaaaag atcgattgcc gtctcgacga
      241 atgcgcccag gccctcaagc tcgcggttga ccgcgatgcg caacgcctgg aacgtccgcg
      301 tcgccgggtg aatcctctcg ccgcgcttgt ggccgacggc gcgcgccacc agctcggcca
      361 gctcgcccgt ccgcttcaag ggtttgccct gctcccgccg ctcgacgatc cagcgcgcga
      421 tgcgccgcga cttgcgctcc tccccgtact cgtagatgac gcgcgcgatc tcttcttcgg
      481 ggagccgttc cagcagctca gccgccgtct cctcttgccc actcgcgtcc atccgcatgt
      541 cgagcggcgc gtcgtggcgg aagctgaagc cgcgctcggc ggtgtcgaac tggagcgacg
      601 agacgccgag gtctgcgagc acgccgctcg cctcccgttc gcccgcctct cgcaccaccc
      661 gcgcgatctc ttcgtgcttc gcgtgcaccg cgcggaaccg cgggccgaag cgcgcgagac
      721 gttcgccggc gaggcgcagc gcctctgggt cgcggtcgat gccgaggacg cgcgcgtcct
      781 cggaggcttc gaggatcgct tcgctgtgcc cgccaagacc gagcgtcgcg tccacgaaca
      841 gcccgccgcg ctccggcgcg agcaaacgga cggtctcaag taacaacacg gggcgatggg
      901 gcgtgcccgg cccccctccg gactccgcca ttaccaccgc cccgtggtct caaagagcag
      961 cggcgctccg agggtgaagg ttcgaggctt caatccaccc cgaaccgcgt gactcacttc
     1021 ggccatgatc ttgccagcca tcaccctcac gcgcgcccgc cctgcccttc agattccgag
     1081 ccgcgccatc gcggcctcgt cctcttccgt gtaggggtct gaaagcaggc gctgctcgaa
     1141 gcgctccagc tcccagactt ccacgtatgt caaatatccg agtacagcca cgtcgcccac
     1201 gacgcccgcc gccttcctga gcagcgggtg gatcaggatg cgcccctgcg cgtccatctc
     1261 ggcctgctgc ccgtagtagt tggttctgtc aaggaacttc cgccgcgccg ggtccatcgt
     1321 ggggacgagg ttgagtctct gctctattga ctcccactcc ggcaggggat aaattctggc
     1381 gcagtctccc gtcaggctcg tgacgtaaac ttcggcgccg tacttgtcct ttatatatct
     1441 gccgaaagcg gtcggcactt tcaaccgccc cttcgagtcc atccgcgccg tgtaatttcc
     1501 tctcagcatg ggagttgcgg cggatttgcg gggcgcgagc tgcttaaaac ttaaagtgac
     1561 actctaaaca ttcccggcaa cctgctctga gtagcccact tttagccact tttgaccacc
     1621 cgccgcgcat agtacgcctc tacgcttaag catgtcaaga ataaaaattg tggaaaactg
     1681 agatttttaa gcacgatttg gtgtattcac gtttaagctg ccgcgtagcc aactgccggg
     1741 gcttcctgta tagttgcgtc tcaagctcgt acaaataagt ggagaattcc ggattttgct
     1801 gaaccgcgga tgaatttcgg cgagaccctg tttttcggcg ccctcgtggc gctggccaac
     1861 gttctgggcg ggctgatact ggcgcgcccc ggcgcgcccg tccgcaaaac gcaactgctg
     1921 agatacctcg tggcgctggc ggcgggcttc atgttggcgg cgatttttat cgagatcgtt
     1981 ccggaatcgg tcgaaatctg ggcggggcag gagggagggg cggcacaggg tactttgtcc
     2041 gcgatgatgc tgctgctcgg gggctacctg ctgattcact tcttcgagca caccatcgcc
     2101 ccgcacttcc acttcggcgc cgagacgcac ccggaggcgg tcgtccgccc gtccgccgcg
     2161 tacgcggcca tcggggggct gtcgatccac acctttttcg atggggcgtc ggtggccgcc
     2221 gccttcgcgg ttgaccgcaa ggtggggctg ctggttttcc tcgcggtgct gctgcacaag
     2281 gttccggagg gcttcacggc ggcctcgatc atgctggcgt cggggcggac gacgcggcag
     2341 gcttttcgcg cgacgctctt catcggcgcg gcgacgatgg tcggcgtgct gagcgtggcg
     2401 ctcttgcaga cgcaggtgaa ggaggcggtc gtgtacgcgc tccccttctc ggcgggcgtg
     2461 acgctgtacg tcgcggcgag cgacctgata ccggaggtca accacctcga agagaagaac
     2521 ccgctcgtct cgctcgtggt cttcgtcggc gcggcgctct tctacgcgct gcacctgctg
     2581 atcgagggct gagcggcgac ggctcgacgc tgaaccggcg cgcgggggga acagtttggt
     2641 gacattttcg ttctatgtta gtactgaaat actgaagtga taacacgatc ccgacgcacc
     2701 tggttttcga ccgccgttct cgcggcggtg tgtctgctcg cgtgcgcgaa tgcggcgcgc
     2761 gcgcaggaag cgccgcgcgc cttttcgcag tcgccgctgc ccgcgcccac cggcttcgtc
     2821 aacgacttcg ccaacgtgat cgacgaagcg acgcaggcgc ggatggagcg ggtgctcacc
     2881 agcctcaggg acaaagcaca gatcgagttc gcggtcgtga ccgtcccgac gactggcggg
     2941 caggacatct tcaactactc gctcgcggtg gcgcgcgggt ggaagatcgg cgcgccggag
     3001 ggcgagaaga aggggctgct cttgctggtc gcggtcgaag accgcaagta ccagatacag
     3061 gtgagccgcc acctggaagg cgacctgccc gacggactgg tcggcgaggt cgggcggcgg
     3121 atgcgcgacc cgttccgcgc gggcgactac agcaaaggtc tgatggacgc cgtgcagacc
     3181 ttcatcgcca ccatcggcaa gcagcgcggc ttcacgttcg aggaggttga ccagtcttac
     3241 gcttatcaac agacgcaacg cacgcgcccg accggcacga ggcggacgag gcagggcgga
     3301 ctcggactcg gcgcgtgctg cttga
//
LOCUS       DBNGOH010000991         3507 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3631272, whole genome shotgun sequence.
ACCESSION   DBNGOH010000991 DBNGOH010000000
VERSION     DBNGOH010000991.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3507)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3507)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3507
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3631272"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            126..620
                     /locus_tag="AC159P_19270"
     CDS             126..620
                     /locus_tag="AC159P_19270"
                     /inference="COORDINATES: protein motif:HMM:NF016836.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HIR3697635.1"
                     /translation="MREMSRDVCLLLTGAGIGAAIMYILDPDRGNRRRALARDKLVAA
                     GNKTSMYAGKLSRDLGNRAQGLVAETRARFRGEQVSDDVLVDRVKAELGRHAVHHRAL
                     DITANGGRVTLQGPALASEVDELLSAVASVRGVQGVDNQLEVHERADGISSLQGEVAA
                     AASA"
     gene            936..1913
                     /locus_tag="AC159P_19275"
     CDS             936..1913
                     /locus_tag="AC159P_19275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008836563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR3697636.1"
                     /translation="MAKTTKKQAAKKAGSRKGAAKKGAAGKAAKKGASRPRVQEEPMS
                     PMPAQHQEKPGIESKMRPRPEYEAPLYTGSGKLRDRVALITGGDSGIGRAVAVLFARE
                     GANVGIVYLDEEQSDAEVTRGAVEAEGRSCLLIPGDVRDAGFCREAVERTVAEFGQLD
                     ILVNNAAFQQHRKRLEDVSEEQWDTTFKTNIYGYFHMTKAALAHMKEGSSIVNTGSIT
                     GLQGSKELLDYSATKGAIHAFTKSLAQNLVERRIRVNCVAPGPVWTPLNPSDKPAAKV
                     AEFGADTPYERPAQPEEIAPAFVFFASEADSSYITGEVLTLLGGETTAG"
     gene            1965..2141
                     /locus_tag="AC159P_19280"
     CDS             1965..2141
                     /locus_tag="AC159P_19280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697637.1"
                     /translation="MSDKDRNKKEGQRTDKKNQARREHENDAVQQAQPPQQAKDASNL
                     TDTQPGPGDDTREP"
     gene            2270..2422
                     /locus_tag="AC159P_19285"
     CDS             2270..2422
                     /locus_tag="AC159P_19285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005370663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallothionein"
                     /protein_id="HIR3697638.1"
                     /translation="MADELDEKTCAHQKCVCVVRGEKRYCSDYCEDAADSDPTSECTC
                     GHPGCG"
     gene            2638..2934
                     /locus_tag="AC159P_19290"
     CDS             2638..2934
                     /locus_tag="AC159P_19290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013564395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HIR3697639.1"
                     /translation="MTYVVTEDCVDCKYTDCAAVCPVEAFHELPDRLYINPETCIDCD
                     ACVPECPVEAIYADSTVPAHLQEWTALNEEAANYPIISGKIPALFGPTCKGNPE"
     gene            complement(3049..>3507)
                     /locus_tag="AC159P_19295"
     CDS             complement(3049..>3507)
                     /locus_tag="AC159P_19295"
                     /inference="COORDINATES: protein motif:HMM:NF024764.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3697640.1"
                     /translation="GIGMPGHFIVRASDADGAGTLVDPFDGRNIEADDCQERLDAIYG
                     GQVALSEKHLRPVGSRAILARILGNLKAVYAQANLHRRALAAVERILLVSPHAREERR
                     DRGLLLAELDRLPEAVVELRRYLKSTPDTPDAESVREQLKKVQARLASLN"
BASE COUNT          673 a         1136 c         1159 g          539 t
ORIGIN      
        1 aatgttctcg acacatcccc cgagaagtat cccccgaaag atgaccgagc acgttccctg
       61 ccgtccgact cgtgcgtgac gtgcggcttc ggtggctatt cgtattcgca agggaggagt
      121 gagcgatgag agagatgagt agagatgtgt gcctgttgct gacgggcgcc ggcatcggcg
      181 cggcgatcat gtacatcctt gaccccgaca ggggcaaccg ccgccgcgcg ctggcgcgcg
      241 acaaactggt ggccgccggc aacaagacga gcatgtacgc ggggaagctg tcgcgcgacc
      301 tcggcaaccg cgcgcagggg ctggtggccg agacgcgcgc acgcttccgc ggcgagcagg
      361 tctcggacga cgtgctggtt gaccgcgtga aggccgaact cggcaggcac gccgtgcatc
      421 accgcgcgct cgacatcacc gcgaacggcg gtcgcgtcac gcttcaggga ccggcgctgg
      481 cgagcgaggt ggacgagctg ctctctgccg tcgcctcggt gcgcggcgtg cagggcgtgg
      541 acaaccaact cgaagtccac gagcgggccg acggcatttc cagcttgcag ggagaagtcg
      601 ccgcggcggc ctcggcctga accggagggg caagaggcga aaaggggtca aggcgaaagg
      661 gtgaaagggc gaagaggtga aaagacgaca ggtttgattg gtcctcccct cttccccact
      721 tcccctcttc cccttttaac ctttgcccct tcattccccc tcggtctggc tcccgctgtt
      781 cccgtgccat tttccgaaca tccccctaac agaaaaatca aacgggtcgc gcatactcgc
      841 gcgcgtcgtc cctttcgcgg agtgttgtcg gcggcgggcg acgtttgcgg cgaaagattt
      901 tttatttcga gactggacga accacgggag gattgatggc aaagacgacg aagaagcagg
      961 cggcgaagaa agcggggagc aggaaaggcg ctgcgaagaa gggcgcggcg ggtaaagccg
     1021 cgaagaaggg cgcgtcgcgc ccgcgcgtgc aggaggagcc gatgtcgccg atgcccgcgc
     1081 agcatcagga gaagccgggc atcgagtcga agatgaggcc gcggcccgag tacgaggcgc
     1141 cgctctacac aggctcgggc aagctgcgcg accgcgtggc gctcatcacg ggcggcgact
     1201 ccgggatagg tcgcgccgtc gccgtcctct tcgcgcgcga gggcgcgaat gtcggcatcg
     1261 tctatctgga cgaggagcag tccgacgccg aagtgacgcg cggcgccgtc gaggccgaag
     1321 gccgctcgtg cctgctcatc cccggcgacg tgcgcgacgc ggggttctgc cgcgaggcgg
     1381 tcgaacgcac ggtcgcggag ttcggccagc tcgacatcct cgtcaacaac gccgccttcc
     1441 agcagcaccg gaagcggctc gaagatgttt cggaagagca gtgggacacg accttcaaga
     1501 cgaacatcta cggctacttc cacatgacca aagcggcgct cgcgcacatg aaggaaggaa
     1561 gctctatcgt caacaccggc tcgatcacgg ggctgcaagg cagcaaggag ctgctcgact
     1621 actcggcgac gaagggcgcg attcacgcct tcaccaagtc gctggcgcag aacctcgtcg
     1681 agcgcaggat tcgcgtcaac tgcgtcgcgc cggggccggt ctggacgccg ctcaacccct
     1741 cggacaagcc cgccgcaaag gtcgccgagt tcggcgccga caccccttac gagcgccccg
     1801 cgcagcccga agagatcgcc cccgccttcg tcttcttcgc ctccgaggcc gattcgagct
     1861 acatcaccgg cgaggtgctc acgctgctcg gcggggagac gaccgcgggc tgaagaagca
     1921 cgactttcat caaccgatct ttcatcgacc gaggggagaa gaccatgtca gacaaagacc
     1981 gcaacaagaa ggaaggccag cgcacggaca agaagaatca ggcccgccgc gagcacgaga
     2041 acgacgcggt gcagcaggcg cagccgcccc agcaggcgaa ggacgcctcc aacctcacag
     2101 acacacagcc cggccccggc gacgacaccc gcgagccttg agctgattcg gcggacaggc
     2161 gctctctgtg cgctctgcgc tcctccgcgc cctctgcgtt gaaatatgcc gaacccatcc
     2221 ttcaacgcag agggggcaga gagttttcga cgcaacggag gttttcaaga tggcagacga
     2281 gttggacgag aagacgtgcg cgcaccaaaa gtgcgtgtgt gtggtgcggg gcgagaagag
     2341 gtattgcagc gactactgcg aggacgcggc ggactccgac ccgacttccg aatgcacctg
     2401 cggccacccc ggctgcgggt aggcccggac tcggcccgtc gaggacagcc cggcggcagg
     2461 cgggcgccga aagataacgt gcccgcctgc cgccgcctaa tcttccgagg ggttcgcacc
     2521 gtcgcccgcg ttcctgatgc cgagtctcct gttgacatca ggctcccgcc tgtcatagaa
     2581 gcagtgtgtc tcaagtcatc acgcccgccg ccggagcggc acgaggagga accggcgatg
     2641 acctacgtcg tcacggaaga ttgcgttgac tgcaagtaca cggactgcgc cgccgtctgc
     2701 cccgtcgagg cgttccacga gctgcccgac cgcctctaca tcaaccccga aacctgcatc
     2761 gactgcgacg cctgcgtccc cgagtgcccc gtcgaagcca tctacgcgga ctccaccgtc
     2821 cccgcacacc tgcaagagtg gacggcgctc aacgaagagg ccgccaacta ccccatcatc
     2881 agcggcaaga tacccgccct cttcggcccg acctgcaaag gcaacccgga atagtctgaa
     2941 gactgaagtc ggaagtctga agtcggaaca aaaggcgtgc tgatgtgact ccgcactccc
     3001 tatgctgttt gttctgacct cagatttccg acttcagact tgtttctgtt agttgagtga
     3061 ggcgagccgc gcctgaacct ttttcagttg ctcgcggacg ctctcggcgt cgggcgtgtc
     3121 gggcgtggat ttgaggtagc ggcgcaattc gacgacagct tcgggcaggc ggtcaagctc
     3181 tgcgaggagc agcccgcggt cgcggcgctc ttcgcgcgcg tgcggtgaga cgagcaggat
     3241 gcgctcgacg gcggcgagcg cgcggcggtg caggttggcc tgcgcgtaga ccgccttcag
     3301 gttgccgagg atgcgcgcga ggatggcgcg gctgccgacg ggccggaggt gcttctcgga
     3361 cagagcgacc tgcccgccgt agatcgcgtc gagccgctcc tggcagtcgt ccgcctcgat
     3421 gttgcgcccg tcgaagggat cgaccagcgt gcccgcgccg tcggcgtccg aggcgcgcac
     3481 gatgaagtga cccggcatcc cgatgcc
//
LOCUS       DBNGOH010000992         2655 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_516626, whole genome shotgun sequence.
ACCESSION   DBNGOH010000992 DBNGOH010000000
VERSION     DBNGOH010000992.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2655)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2655)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2655
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_516626"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..857)
                     /locus_tag="AC159P_19300"
     CDS             complement(<1..857)
                     /locus_tag="AC159P_19300"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF018305.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3697641.1"
                     /translation="MNDAEPGPRPRAVWAVVLGIWTFFGVLNASQVFIGMRAEGMDHS
                     FGTMLAWQLANWYLWALLTPLIIRLGRRFPFERATWARALAVHFLFCALAAVAHSALH
                     NLATIYLRPFGALTNERPFLDMFWGRLASQFHLYVLIYGTILGAGYAFDYYFKFRERA
                     RQAARLEAQLSQAQLQALRMQLNPHFLFNTLNGIAGLVRDNRNKTAVQMIAGLSDLLR
                     HALDSAGQQEVPLRRELEFLELYLDIQQMRFSDRLKVEMEIAPETLDARVPNLILQPL
                     VENAIRHGVA"
     gene            complement(1146..2414)
                     /locus_tag="AC159P_19305"
     CDS             complement(1146..2414)
                     /locus_tag="AC159P_19305"
                     /inference="COORDINATES: protein motif:HMM:NF024753.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HIR3697642.1"
                     /translation="MLLNHRGRNATTRALGFVAASLVLSLAAAAQGSDWPQWGGPARD
                     FKSETKGLAAAWPEGGPRRVWSRELGEGYSAIAAEGSRLFTMYRRGAQDVVVALDAAT
                     GKTLWEYAYDAPFSAEYSMENGPGPHATPTVAGAHVFAAGPTGKLHALDKKTGKLLWS
                     HDLMTEFKGTVRVNGYACSPLVYRDTVIMMVGGAGNALVAFEQKTGRVVWKKHDFRNS
                     PGSPVVINVGGQDQLVAFMWDTLVGVEPRTGELLWTHPHQTEYGLNVSTPVWGAEDNL
                     LFVSSSYGGGSRVLKLAREGAKTTVEEVWAHKLMRVHYSNAVRLGDYVYASSGDFGTA
                     PLTAVNVKTGKVAWRDRTLARASFVAAEGRLIMLDEDGHLALATPTPEGLKLHSKIEL
                     LKSNAWTVPTLAGTRLYVRDRRTIMALDLK"
BASE COUNT          422 a          876 c          915 g          442 t
ORIGIN      
        1 gcgacgccgt ggcggatggc gttctcgacg agcggctgca agatgaggtt cgggacgcgc
       61 gcgtccagcg tctcgggcgc gatctccatc tcgaccttga ggcggtcgga gaagcgcatc
      121 tgctggatgt cgaggtagag ttcgaggaac tccagttcgc gccgcaaggg cacctcctgc
      181 tggcccgccg agtcgagcgc gtggcgcaaa aggtcgctga ggcccgcgat catctgcacc
      241 gcggtcttgt tccggttgtc gcgcactaac ccggcgatgc cgttcaaggt gttgaagagg
      301 aagtgcgggt tgagctgcat ccggagcgct tgaagctgcg cctgcgagag ctgcgcttcg
      361 agccgcgccg cctgccgcgc gcgctcgcgg aacttgaagt agtagtcgaa ggcgtagccg
      421 gcgccgagga tggtgccgta gatgagcacg tagaggtgga actggctcgc cagccggccc
      481 cagaacatgt cgaggaacgg gcgctcgttc gtcagcgcgc cgaacggcct gaggtagatg
      541 gtggcgaggt tgtgcagcgc cgagtgggcc acggcggcga gcgcacagaa gaggaagtgg
      601 acggcgagcg cgcgcgccca cgtcgcgcgc tcgaacggga agcggcgccc gagccggatg
      661 atgagcggcg tcagcagcgc ccataagtac cagttcgcga gctgccacgc gagcatcgtc
      721 ccgaagctgt gatccatccc ctcggcgcgc atgccgatga agacctggct cgcgttcagc
      781 accccgaaga acgtccagat gccgaggacg acggcccaca ccgcacgggg ccgcggcccc
      841 ggctccgcat cgttcatgtc ctctctgaat cgcggtgctg tcatccggtt gtcgtgtgta
      901 ccttttgtgt ttcgccgcgt cgcggcggaa tgagtttagc ccggcctttc aaggccggga
      961 ttggtatcag ccaaccatcg gcgtcgcgta gcgacgcgtg aatgtgacgc atccattcag
     1021 gcgtcgctga cgcgacgcgg gccgcttacg tcatgcctga cccggcgttg aaacgccggg
     1081 ctaaaatcaa acgccgctac gcggcgcgtg gtcgcacgcg cttcaacctc aacgtctcac
     1141 gcgcttcact tcagatcgag cgccatgatg gtgcggcggt cgcggacgta gagcctggtg
     1201 ccggccagcg tcggcaccgt ccaggcgttg ctcttgagca actcgatttt cgagtgcagc
     1261 ttcaagcctt cgggcgtcgg ggtggcgagc gccagatggc cgtcctcgtc gagcattatc
     1321 aaccgcccct cggcggcgac gaaagaggcg cgcgccagcg tccggtcgcg ccacgcgacc
     1381 ttgccggtct tgacgttgac ggcagtcaac ggcgcggtgc cgaagtcgcc gctcgacgcg
     1441 tagacgtagt cgccgaggcg caccgcgttg ctgtagtgaa cccgcatcag cttgtgcgcc
     1501 cacacctctt cgaccgtggt cttcgcgccc tcgcgcgcga gcttcagcac gcggctcccg
     1561 ccgccgtagg acgaagagac gaagagcagg ttgtcttccg cgccccagac aggcgtgctc
     1621 acgttgagac cgtactcggt ctggtgcggg tgcgtccaga gcagctcgcc cgtgcgcggc
     1681 tcgacgccga ccagcgtgtc ccacatgaag gcgacgagct gatcctggcc gccgacgttg
     1741 atgacgacgg gcgagccggg cgagttgcgg aagtcgtgct tcttccagac gacgcgcccc
     1801 gtcttctgct cgaaggccac gagcgcgttg cccgcgccgc cgaccatcat aatcaccgtg
     1861 tcacgataga cgagcgggct gcacgcatag ccgttgacgc ggacggtgcc cttgaactcc
     1921 gtcatcaggt cgtgcgacca gagcagcttg cccgtcttct tgtcgagcgc gtggagcttc
     1981 cccgtaggcc cggcggcgaa gacgtgcgcg cccgcgaccg tcggcgtcgc gtgcggcccc
     2041 ggcccgttct ccatcgagta ttcggcggag aacggcgcgt cgtaggcgta ctcccacagg
     2101 gtcttgcccg tcgccgcgtc gagcgcgacg acgacatcct gcgcgccgcg ccggtacatc
     2161 gtgaagagcc tgctgccctc cgccgcgatg gccgagtagc cctcgcccag ctcgcggctc
     2221 cacacgcggc gcgggccgcc ctcgggccac gcggcggcca aacccttcgt ctccgacttg
     2281 aagtcgcgcg cggggccgcc ccactgcggc caatccgagc cttgcgccgc cgccgccaaa
     2341 gacaacacga gcgacgcggc gacgaagccg agcgcgcggg tggtcgcgtt acgtccgcga
     2401 tgattcaaga gcatgtcggc accttcgttt cgttagttac tgcttcgact cgtgacggct
     2461 gcgcggtcgt ccggcgcgtc actgctgcgt ggcggtggcg ggcttcggtc gctcgaaaag
     2521 tgcgggcgcg acggccccgt tgacgacgat ggattcggcg gtgaaggaga gcgacggctc
     2581 ggccacgccg ttgaaggtgg ctgtggtctt gaagggcagc gtcagccctt cgaccgcgcg
     2641 gaagtcggag aaggt
//
LOCUS       DBNGOH010000993         1887 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2716295, whole genome shotgun sequence.
ACCESSION   DBNGOH010000993 DBNGOH010000000
VERSION     DBNGOH010000993.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1887)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1887)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1887
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2716295"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(121..729)
                     /locus_tag="AC159P_19310"
     CDS             complement(121..729)
                     /locus_tag="AC159P_19310"
                     /inference="COORDINATES: protein motif:HMM:NF019265.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR3697643.1"
                     /translation="MASPRNPETDKATSTRDPRLAEFIKSISAGDEQALAAFYDATSR
                     VVYSLLLRVLAAREEAEEVLLDVYTQVWRQAGAYNEGRGTPLAWLTTIARSRAIDRLR
                     SRGFERRHTDPLDAATERASGARDVEETAAAGETGRRVRAALDALPPEQREVIELAYY
                     GGMSHGEIAAHTGHPLGTVKTRTRLGMMRLREMLKETYEATR"
     gene            948..1502
                     /locus_tag="AC159P_19315"
     CDS             948..1502
                     /locus_tag="AC159P_19315"
                     /inference="COORDINATES: protein motif:HMM:NF013331.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005344 - oxygen carrier activity
                     [Evidence IEA];
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group I truncated hemoglobin"
                     /protein_id="HIR3697644.1"
                     /translation="MLKVKGFAAALLLAAAFAAQASAQETAAKKSLYERLGGADAISA
                     VVDDFAGRVLKDEKINKKFAKSDAPRLLYHLKQQVCAATGGPCKYTGLSMKQSHKNMA
                     VTEGEFNALVGHLVDTLAKFNVPEAEKNELLGILGGLKDQIVEVKGDATGTPLPDNFK
                     PAKPLSEKKIKEGPFGKSEAKPKM"
     gene            complement(1623..1841)
                     /locus_tag="AC159P_19320"
     CDS             complement(1623..1841)
                     /locus_tag="AC159P_19320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697645.1"
                     /translation="MYTKPMVLPLTADGKIYALLDERGNQIASGSREVCRTLLCLLSM
                     PLMERPARHVRGGASSVTWRERTRATRN"
BASE COUNT          323 a          612 c          651 g          301 t
ORIGIN      
        1 aagaggcaca gccctccgag aggtgcgctt cgaaggcgcg cgcctcgtgc tgcgagagcg
       61 cgccgagtgc gtagagcgcc gcacgctccg gccccgattc gtgttccggc cccgtgtgct
      121 tcatcgcgtc gcctcgtagg tttctttcag catctcgcgc agacgcatca tgccgagccg
      181 cgtgcgcgtc ttgaccgtgc cgagcgggtg gccggtgtgc gccgcgatct cgccgtggct
      241 catgccgccg tagtaggcga gttcgatcac ctcgcgctgc tcgggcggga gcgcgtcgag
      301 cgcggcgcgc acgcgcctgc cggtctcgcc cgccgccgcc gtctcctcga catcgcgcgc
      361 gcccgaagcg cgctcggtcg ccgcgtccag agggtcggtg tgccgccgct cgaaacccct
      421 tgagcggagg cggtcgatgg cgcggctgcg cgcgatggtc gtgagccagg cgagcggcgt
      481 cccgcgcccc tcgttgtacg cgccggcctg ccgccagacc tgcgtgtaca cgtcgagcaa
      541 gacctcctcg gcctcctcgc gcgcggcgag gacgcggagc aggaggctgt agaccacgcg
      601 gctcgtcgcg tcgtagaatg cggcgagagc ctgctcgtcg ccggcggaga tgctcttgat
      661 gaactccgcg aggcgcgggt cgcgcgtgct ggtggccttg tccgtctcgg gattgcgggg
      721 tgacgccaag gggtcccttc ctgtctcccc tctcgtagga ctacgccgcg ggcagttccg
      781 gcggattcaa tttaacaaaa acgtgcgcgg tatccaaaaa tccgttccgc gcgcgccccg
      841 cgtagcttcg attaacaagc ggtcaagcga caacggaata atcgccgcgc ccttccggcg
      901 cgggcgctcg gaaccccaac caaacagaca aaggagagga gagaatcatg ctgaaagtca
      961 aaggcttcgc cgcggccctg ctgctcgccg ccgcgttcgc ggcgcaggcg tctgcgcagg
     1021 agacggcagc gaagaagtca ctctacgagc ggctcggcgg cgcggacgcc atctcggcgg
     1081 tggtggacga cttcgccggg cgcgtcctca aggacgagaa gatcaacaag aagttcgcca
     1141 agtcggacgc gccgcgcctg ctctaccacc tcaagcagca ggtctgcgcg gcgacgggcg
     1201 gcccctgcaa gtacacgggc ctctcgatga agcagtcgca caagaacatg gccgtcaccg
     1261 agggcgagtt caacgcgctc gtcggccacc tcgtggacac gctcgccaag ttcaacgtcc
     1321 ccgaggcgga gaagaacgag ctgctcggca tcctcggggg tctcaaggat cagatcgtcg
     1381 aggtgaaggg cgacgcgacc ggcacgccgc tgcccgataa cttcaaaccg gcgaagccgc
     1441 tcagcgagaa gaagatcaag gaagggccgt tcggcaagtc ggaagccaag ccgaagatgt
     1501 aagcggcggg acagagcgaa ggggcgcgga cgagatgtcc gcgccccttc gctgttgggg
     1561 ggtaaaactt tcgccggtcg ggcgacgggg gaataatctt attggggtcg ggggcagcgg
     1621 tctcagtttc tggtcgcgcg tgtcctttcc ctccacgtca cggagctggc cccgccgcgc
     1681 acgtgacgcg cagggcgctc catcaggggc atggaaagta ggcagagcag cgtgcgacac
     1741 acctctcggg agccggatgc gatctggttg ccgcgctcgt cgagcaaggc atagatcttc
     1801 ccgtcagccg tcagaggtaa gaccatcggt ttggtgtaca tgctgtttcc aggcagtgtt
     1861 actgcaggta gcgtgacagg cggcgtc
//
LOCUS       DBNGOH010000994        18288 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_149872, whole genome shotgun sequence.
ACCESSION   DBNGOH010000994 DBNGOH010000000
VERSION     DBNGOH010000994.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 18288)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 18288)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18288
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_149872"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..704)
                     /locus_tag="AC159P_19325"
     CDS             complement(<1..704)
                     /locus_tag="AC159P_19325"
                     /inference="COORDINATES: protein motif:HMM:NF012686.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HIR3697646.1"
                     /translation="MRSTVQTVDYVVLVGYLCLMAGVGAYFMRYMRGGNDFLKGGNRI
                     EWWVAGMASFMSGFSVWTFTGGAGFAYQHGIIGTFLLSLAVPAFLLGYLVYAKLWRRS
                     RVTTVVEYIRERFGDGTHKTFSWMTGPSQLMFASVRLFALSSFMSFALGVPVPYLIVV
                     CGLVILAYTVLGGYWAVCFTDMFQFMFLFPVAVVLMILSLADVGGVAGLTARAPEGFF
                     DVFGGEYGWLFLVAYT"
     gene            complement(765..1793)
                     /locus_tag="AC159P_19330"
     CDS             complement(765..1793)
                     /locus_tag="AC159P_19330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007360101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HIR3697647.1"
                     /translation="MNENECVILLDPHPRAIDLIFSQEDRARLERLGRVVWHDGSQAP
                     DEHVERYLPEAVAVIGQTPLPRERLDRAPHLRLVANVESNFLSNVDYEECHRRNIHVV
                     ATSPVFAQPVAEMALGMAICAARRIHEADAAVRRGNESLYGEADNHDSFLLHGKTLGL
                     LGCGNLGRALLPLLGAFSRDIIVHDPWLHPNVLRELGVEPVGFEELFERSRVLFVLAA
                     VTTENRGAIGAHQFDAMQTGAVFVLVSRAGVVDFDAMLDAAERGQVRVAVDVFPDEPI
                     PPDARARRTPNTLLSAHRAGNIPEIWHGMGTMVVDDLELVLQGLPPQRCTRATPETAA
                     RLRSKPIS"
     gene            complement(2051..2806)
                     /locus_tag="AC159P_19335"
     CDS             complement(2051..2806)
                     /locus_tag="AC159P_19335"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006043 - glucosamine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HIR3697648.1"
                     /translation="MKVEVFDSKSEMGAAAARDAAAIIRAAIGERGEARVIAATGASQ
                     FEFLDALVGEVGIDWGRVVLFHLDEYVGLPESHPASFRCYLEERLAGRVRPGAFHFID
                     GEAADPRAECRRLGGLISRREVDVAFVGIGENGHLAFNDPPADFETDEPYLVVVLDEA
                     CRRQQVGEGWFASLDEVPARAISMSVRQILKARRVICVVPDARKARAVRDCLESAVSP
                     HHPASVLRRHEGASLYLDRDSASLLKNRAARKG"
     gene            complement(2817..6791)
                     /locus_tag="AC159P_19340"
     CDS             complement(2817..6791)
                     /locus_tag="AC159P_19340"
                     /inference="COORDINATES: protein motif:HMM:NF020121.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase trimerization
                     domain-containing protein"
                     /protein_id="HIR3697649.1"
                     /translation="MRKLLSLALSALVLASLVPDTRARQTGVGGSSFHKNDITYSTEV
                     ATPHVPWATKLQGGPVKGFFIPSIQYGRDMVELMQRLQLAPTTVSIDRSWDINCWGIG
                     DFYDHVHRGDRDDFRIVYGYVEKDLTGPAPFEVMVIPGLNGWSRMTRPARDAVLRRVR
                     DGAGLVLLHPFLGDVKGHPFKGDEAEADARIWELSPLVGVPDDTVNERGYPEVNQDAV
                     TRGRWEAARPHFITEGLPLELLPEGQAGGSFYKYKAAGDVIIRSGEHPVAAVKTYGKG
                     RVVAFAYVEEGFTPQSVNPFETKTYWDYWEYQYSLLSRAVLWAAGRDAGVRIDSLAAD
                     AAGRPSVKLALTSDAARRVEIEVSGKSEFGQRLGSHKVTKELAAGRNSFELAPAAAAD
                     GGGLAGGRQIYDLILRDARTGATLNWGSAVRHAPKRAMVTQAKSAVDVYKRGETLSAV
                     VRATGNLEGLRMRLKVSDDLGRLLTVLEAPARGERTFSYALQDFVGKQAFVTAELADA
                     GGAVVDQLRAKPVLVVQAKRRAREYTALVAFGGTKHYLQDAQMRQVRAVAADTGFTWS
                     GGDVDNSLNVPRGTFGVYWYDRGPTTPEGLERAIEDYKRTGDFDALGYLTKKELYKRT
                     GDTKFLRRTPSFNDRTFMDALAGITRATARNKARFNMDYYFVGDEGSLTSYGDPVDFD
                     WSPQALEDFREWLKGEYRTLDALNRVWRSDFRDWDAVLPSTTEQARKSGNFAPWADHR
                     TFMEATFARAYARVRESVVEGDPEARIALSGTQETNAYNGADWYRLDQVIDDFLSYEG
                     GNQWDMHRSFAKPDSMIGFWTGYGSSGIGVQNAIWNAAVNNVLHPNIFWMYSFLDPDL
                     THSNSARDMGLAFRSLRFEGVGKLLMESERLGDGIAIHYSMPSVHGASILGYHRNFDG
                     GADKAPEKAAANFPADRDGWVRAVKDLGLQPDFVASEQIEGGALSAGKYRVLILPLSV
                     ALSAREAKAVEEFARTGGVVIADAAAGLLDEHCAWQPRGVLNELFGISAPESSKRALT
                     RVAGAAEVTAEGAGWGLKADELADLAAAESDVKAAGGRALARVGGADAVVARQVGQGW
                     TFYLNALFDRYPKLRGEKSGGAGHRALLARLLERAGVRPAVEVLTADGKRLAQAQVVR
                     YRFGDSEILAVVKENVGVAGVVGQDGVTVYNDAGLGQVARQEITVKLPRAFYVTDVRT
                     GKRLGRTDAVRTSLVVGDALVLGLSHAENALTLSGPSAARLGEHPEFAVASAGAGRRL
                     FRCQVVAPDGSMLPAYSKNVPAEGGRATFTLPSALNDAPGAYTVRVTDVVTGASAAAV
                     LTLK"
     gene            complement(6913..7878)
                     /locus_tag="AC159P_19345"
     CDS             complement(6913..7878)
                     /locus_tag="AC159P_19345"
                     /inference="COORDINATES: protein motif:HMM:NF013567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR3697650.1"
                     /translation="MTLRLALVGCGEHSESSHAAPLRRYAAARPGGIELAAACDLDRA
                     RAERFCREFGFARAYASLDELLAAERLDGCVTVMPVDRIGEVAAKVLERGIPCVIEKP
                     LGTSLAEVERLAEVAGRTGTPHMVSVNRRFMPYLNRASAWAREAGGLLYARATMVRQR
                     RDEPAFIWETALHALDALRHVAGEIKDYRATLHRPEGLAANWYLVSLDFEGGARGQLE
                     VLPTAGVIEESYELFGEGFRARVVAGSGPQRSLHCWRGGRLETEEFADETAPEDLRSG
                     AYEEVLEFVGALREGRRPAPTVADVLPSSRVCFRIAEELTGQGRG"
     gene            complement(7903..8856)
                     /locus_tag="AC159P_19350"
     CDS             complement(7903..8856)
                     /locus_tag="AC159P_19350"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008443 - phosphofructokinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HIR3697651.1"
                     /translation="MIICVSANPAIDRRLRLGRLHAGAVNRATSVCAMTGGKAAHVAM
                     AAAALGERVTWVGFLGGSTGEECERGLVSLGIPMAVVKTAAETRMNMEIVEADGTVTE
                     VLEPGGHVSEGEVERMLSLCRDLFTEYRGAAQVALSGSLPPGAPADFYAQLVRLVHAH
                     GCRALLDTSGEALRAALSESPDLVKPNRDEAEALTGVRLDGARAAAEAARLLFEAGAR
                     GVAISLGAEGMFWQATRDSAPFVARPPRVEARSAVGCGDASLAGFAVAHRRGLSGREF
                     VRFAAACGTANCLADAPGMIDARQVERLVPQAAVESLDGRG"
     gene            complement(8902..9954)
                     /locus_tag="AC159P_19355"
     CDS             complement(8902..9954)
                     /locus_tag="AC159P_19355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR3697652.1"
                     /translation="MKDEPEDKFIAMVSAKAETELRLTDFAPRASLVVPAHDVQRAKY
                     PAIDFHNHLDAQEPREVLRVLDACNIERCVNITMRVGDEAVRVLERFRRAAPDRFSTV
                     AWMDWRDVGEPGFFARSVGRLERMVAKGARGLKVWKDLGLSLRGPEGSLLRVDDERLA
                     PLFERAGELGVFVMFHTADPDAFFQPIDRFNERYEELSAHPDWSFHGSHFTKAELLAQ
                     RNRVLARHPRTRFVGAHVAERPECLAEVAGWLDAHPNFSVDISARASELGRQPRAARE
                     FFDNYSERILFGTDLVPEEEMYRLYFRFLETADEYFDYPSHASRQGRWQIYGLRLPDE
                     VLRRVYRDNALRLLGG"
     gene            complement(10006..11877)
                     /locus_tag="AC159P_19360"
     CDS             complement(10006..11877)
                     /locus_tag="AC159P_19360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020570015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HIR3697653.1"
                     /translation="MNFGALDWFVALFVTAICFLPALLFGRRAGKDTSEFFASGRSVP
                     WWLAGISMVATTFSSDTPNLVTDIVRRQGVAGNWVWWAFVLTGVATVFFYARLWRRSG
                     VLTDLEFYEIRYSGRAASAVRGFRAVYLGLFFNCLIMATVNLAACKIAAIMFGLPRWQ
                     TLLAVGLLNVVFAAHSGLWGVLVIDMVKFFIMMTAVVAAAYFAVYIPEVGGIGGLVEK
                     VSAMSGPGGIRYLDVLPDFTNNWDVAAAVFVLPIAVQWWSVWYPGAEPGGGSYIAQRM
                     LASKSEKDALGAVLFFNVAHYVLRPWPWILVALASLVVYPQLSDIQKAFPDLDPALLG
                     HDIAYPAMLKFLPAGFVGLVVGGLAAANSSTILTHLNWGASYLVHDFYRRFVRTDATE
                     KHYVLAGRVATVLLFVVSSALVFVLDTAKDTFDLILQVGAGTGLLYLLRWFWWRINAW
                     SEVVAMVSSFLISVAFLVLEKQGVYSATTSQRLLMTVGFTTVCWVLAAYLAPQTDRRT
                     LVEFYRKVHPSGPGWESVRREAGIEKGAVERADNIPMAMLGWAAGCAMIWSSLFTVGQ
                     FIYGRYVYGLALLAVFVVSGLVLLRVINRLWSSRSEITGEEGEPTEEEHGEGGARQD"
     gene            complement(11946..12830)
                     /locus_tag="AC159P_19365"
     CDS             complement(11946..12830)
                     /locus_tag="AC159P_19365"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0030388 - fructose 1,6-bisphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HIR3697654.1"
                     /translation="MTSPSEQTATTRGAGRTPVPSRRLLEEARREGRAVAALNFYNAE
                     TLRAHIKAANECGASVILQTTESTIDYLGIKLVVAMARAAAEEIERPVALHLDHGGSY
                     ELAARCVEAGYTSVMIDGSKLPFDENVGLTRRVVELAHAAGVSVEGELGHVAHNEEGG
                     DISRFYTRPDTAARFVEETGVDALAVAVGTAHGFYKGEVRLDFERLEQIRDAVGYAAA
                     LVLHGGSGVPDELMRRAVSCGIRKINFGTELKNAFTLAVKQSLSASDEIDLRKTFAPA
                     IEAVREISSAKIRVCSLL"
     gene            complement(12869..13813)
                     /locus_tag="AC159P_19370"
     CDS             complement(12869..13813)
                     /locus_tag="AC159P_19370"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012515.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HIR3697655.1"
                     /translation="MREEAGSGVVVIGELNVDAVATGLAQEPKLGLEIIAEDFQLTLG
                     SASAIFACGVAKLGHEVTFVSRVGRDDFGDLCLEALRANGIPTRHVLRDPSEKTGVTL
                     ALSTRRDRALVTYLGAISSLRYEDVRMSLLKGKSHLHLTSYFLQEGLRPSFARIFREA
                     RAKGLTSSFDPNSDPTSSWGEDIWNVLEHTDIFFLNRDEALQLTRARDTRGALRALAR
                     RVPCAVVKLGAKGAVAIRDGRVASEPGFRVDALDTTGAGDTFAAGFVSAHLEGLGLEE
                     CLRRGNACGALSTLKAGGTANQPDRAALERFLRAQAGR"
     gene            13963..15138
                     /locus_tag="AC159P_19375"
     CDS             13963..15138
                     /locus_tag="AC159P_19375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HIR3697656.1"
                     /translation="MDELSRLLALSEEERAEQGLVHTPREIYQQPDTWRRTYEDFSAV
                     AGEAQDFLARAGLSGGVAPTVYLVGAGTSDYIGRALASLLRREWRCEVQAVPSTDLLT
                     DMDEHVIPGRPYLWVSFSRSGDSSEGVAVIEAALERHPSVRHLIVTCNGGGRMARDFR
                     GRENVFCLVLDDAVNDRGLAMTSSFTNMVVAGQCLAHVRDLGAYGRVVEELAAAAERF
                     TGAAADAAARLASERHTKFCFLGTGALRAVAVESALKVLELTAGRVYTWSESYLGVRH
                     GPLSAVDDETLVVGFLSGDGRRRAYELDLLREVRAKGLAGKIVAVWPCDASQAGGFDE
                     TLSLGLAAGFEDLHRPPLDVIFGQLFGLFNSLGQGLKPDTPSPKGAITRVVERVRIH"
     gene            complement(15158..15919)
                     /gene="agaR"
                     /locus_tag="AC159P_19380"
     CDS             complement(15158..15919)
                     /gene="agaR"
                     /locus_tag="AC159P_19380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor AgaR"
                     /protein_id="HIR3697657.1"
                     /translation="MLNLERQARILELIEEHGKVEVGDLSRRLRASEVTIRKDLKELH
                     ARGLVRRAHGGAVRAQSVSADPSLQVKAALHADEKRRIGAAAAALVADGESIIIDSGT
                     TTQQVARHIKERRGLKVITNALNVAAELTGAGGVEVILLGGLLRQNSGSLVGQFAEEM
                     LEQLSVDKLFLAVDAVDLEFGLSTPNLDESHVNQAMVRAARETILVADSSKFGRRSLS
                     RIVSLSKVARVVTDHGLPDAMRAGLRELGVELILT"
     gene            complement(15978..16880)
                     /locus_tag="AC159P_19385"
     CDS             complement(15978..16880)
                     /locus_tag="AC159P_19385"
                     /inference="COORDINATES: protein motif:HMM:NF012692.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HIR3697658.1"
                     /translation="MDYAIGIDLGGTNIKAAVVRPSGEVLAESLAETRDDGRAAWAAG
                     VRELIEALEARLGGAAACVGIASPGMAARDGRSMASVSGHLESLQGFDWIEYLGDGRA
                     PALINDGQAALLAEAWKGAAAGARDAVMLTLGTGVGGAVLTGGRLLTGHLGRGGHIGH
                     ICLDVDGEPDSLGIPGTLEEAVGNRTLAKRSRGLFDSTEALVAAHLAGDVNATRVWLR
                     AVYQLACAVTSMINVLVPEVFVIVGGIAQAGAALFDPLERYLGLMEWRPNGHRVRVVP
                     AALGGVAGALGAARHAMLNRPGLC"
     gene            complement(16951..>18288)
                     /locus_tag="AC159P_19390"
     CDS             complement(16951..>18288)
                     /locus_tag="AC159P_19390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3697659.1"
                     /translation="PISMALSEQPGAGRRAPIKRLRWWIGGLLFASTVINYIDRQTLS
                     NLAPFLKQDYGWTNTDYAYLVISFRIAYSIGQTVCGRLVDKLGTRRGLTLSVVWYSVV
                     SMLTSLAGGFYSFAAFRFLLGVGESANWPAATKAVSEWFPKRERGLATALFDSGSSIG
                     AAIAPYLVLGIYFKWGWRPAFIVPGVLGFLWLAAWRWLYHRPEEHPRISAEEREMILR
                     DKQEDEPEGVERVRPRWGQLLRLPQTWGTISARTLTDPVWFFVTDWFPIYLVAKGIRL
                     EEGLFAFWIPFIAADLGNFFGGWLSGHLIKRGWSVGAARKALVVFGGLGVTALIPTVF
                     TTNLLAIALLFGFATFSYASFTTIANVLPSDLFHSESVASVSGLSGTGAGLGTIIAFL
                     LVGYVSDARAGMGTAAFDPIIVVAGLIPLVGMALVLLLVRNTRATEEGLVRRI"
BASE COUNT         2894 a         6287 c         6384 g         2723 t
ORIGIN      
        1 atggtgtagg cgacgaggaa gagccagccg tactcgccgc cgaacacgtc gaagaacccc
       61 tcgggggcgc gcgccgtgag gcccgcgacg ccgcccacgt cggcgagcga gaggatcatc
      121 aggacgacgg ccacggggaa gaggaacatg aactggaaca tgtcggtgaa gcagaccgcc
      181 cagtacccgc ccaaaaccgt gtaggcgagg atgaccaggc cgcagacgac gatcaggtac
      241 ggcacgggga cgccgagcgc gaacgacatg aaggaggaga gcgcgaagag tcgcaccgag
      301 gcgaacatga gctggctcgg ccccgtcatc cacgagaagg tcttgtgcgt gccgtcgccg
      361 aagcgctcgc ggatgtactc gaccacggtc gtcacccgcg accgccgcca gagcttcgcg
      421 tagacgaggt agccgagcag gaaggcaggc acggccagcg agagcaggaa ggtgccgatg
      481 atgccgtgct ggtaggcaaa gccggccccg cccgtgaagg tccagaccga gaagcccgac
      541 atgaacgagg ccatccccgc cacccaccac tcgatgcggt tcccgccctt cagaaagtcg
      601 ttgccgccgc gcatgtagcg catgaagtag gcgccgacgc ccgccatcag gcacaggtat
      661 ccgaccagca ctacgtaatc gaccgtctgc acagtggaac gcatcgctct ccttgctctc
      721 cttctcggcg gacgactccg tccgccacgt ccatggttct ttgtctaaga gatcggcttg
      781 ctgcgcaggc gtgccgcggt ctcaggcgtc gcgcgcgtgc agcgctgcgg cggcagcccc
      841 tgtaagacga gttcgaggtc atccaccacc atcgtgccca tgccgtgcca gatttcgggg
      901 atgttgccgg cgcggtgcgc cgagagcagc gtgttcggcg tgcggcgcgc gcgtgcgtcc
      961 ggggggatcg gctcgtcggg gaagacgtcc acggcgacgc ggacttggcc ccgctcagcc
     1021 gcgtcgagca tcgcgtcgaa gtccaccacg cccgcgcggc tgacgaggac gaagaccgcg
     1081 cccgtctgca tcgcatcgaa ctggtgcgcc ccgatggcgc cccggttctc cgtcgtcacc
     1141 gcggcgagca cgaagaggac acgcgagcgc tcgaacaact cttcgaagcc caccggctcc
     1201 acccccagct cacgcagcac attcgggtgc agccacgggt cgtgcacgat gatgtcgcgc
     1261 gagaaggccc cgagcagcgg cagcagggcg cgacccagat tcccgcagcc gagcagcccg
     1321 agcgtcttgc cgtggagcag gaaggagtcg tggttgtcgg cctcgccgta gagcgactcg
     1381 ttgccgcgcc ggacggcggc gtccgcctcg tgtatgcgcc gcgccgcgca gatggccatc
     1441 cccagcgcca tctcggccac cggctgcgcg aagaccggcg aggtcgcgac cacgtggatg
     1501 ttgcggcggt ggcactcctc gtaatccacg ttcgacagga agttgctctc gacgttggcg
     1561 acgaggcgca ggtgcggcgc cctgtccaga cgctcgcgcg gcaggggagt ctgcccgatg
     1621 acggcgaccg cttcgggcag gtatcgttcg acgtgctcgt ccggggcctg agagccgtcg
     1681 tgccagacca cgcgcccgag ccgttcgagc cgcgcccggt cttcctggct gaagatgagg
     1741 tcgatggcgc gcgggtgcgg gtcgagcagg atgacgcatt cgttctcgtt catacgtccc
     1801 gtccttcagt gtaagtaaac tgaagcgtgt gcctaaatcg ccgcgtcagc ggcgcttgaa
     1861 tgtagcccgg gctttcaagg ccggggccgc cgcacgttaa cgagtgccgc gtcgcgtcag
     1921 cgacgcctga gttcagccgt cgctgacgcg acgcgacggc gttttactcg acctcgaccc
     1981 ggccttgaaa ggccgagcta aactcagccg ccgctccgcg gcgatttatt cacagccttc
     2041 aaataaactc tcagcccttt cgcgccgccc ggttcttcag gagcgacgcc gaatcgcggt
     2101 cgaggtaaag gctcgcgcct tcgtgccgtc gcaggaccga ggcagggtgg tgcggactca
     2161 ccgcagactc cagacagtcc cggacggccc gtgccttgcg cgcgtcgggc acgacgcaga
     2221 tgacgcggcg cgccttgaga atctgtctga ccgacatcga gatggcgcgg gcgggaacct
     2281 cgtcgagcga agcgaaccaa ccttcgccga cctgctgtcg gcggcacgcc tcgtcaagca
     2341 cgacgacgag ataaggctca tcggtctcga agtcggcggg cgggtcgttg aaggcgaggt
     2401 ggccgttctc gccgatgccg acgaaggcca cgtccacctc gcggcgcgag atcagcccgc
     2461 cgagccgccg gcattccgcg cgcgggtctg ccgcctcgcc gtcgatgaag tggaacgcgc
     2521 cgggtcggac gcgcccggcg aggcgttctt cgaggtagca gcggaagctc gccgggtgcg
     2581 actcgggcag gccgacgtat tcgtcgaggt ggaagaggac gacccggccc cagtcgattc
     2641 ccacttcgcc gacgagcgcg tcgaggaact cgaactgcga ggcgccggtc gccgcgatca
     2701 cgcgcgcctc gccgcgctcg ccaatcgccg cgcggatgat cgccgccgcg tcgcgcgccg
     2761 ccgccgcgcc catctcgctt ttcgagtcga agacttccac cttcatcggt cgcaccttat
     2821 ttcagagtca acacagccgc ggcggacgcg cccgtcacca cgtccgtcac gcgcacggtg
     2881 tacgcgcctg gcgcatcgtt gagcgccgag gggagcgtga acgtggcccg cccgccctcc
     2941 gccggaacgt tcttcgagta ggccggcagc atcgagccgt ccggcgcgac gacctgacag
     3001 cggaagagcc tccgccccgc gcccgcggag gcgacggcga actcggggtg ctcgccgagc
     3061 cgcgctgccg aggggccgct gagcgtcagc gcgttctccg cgtggctcag tccgagcacg
     3121 agcgcgtcgc cgacgacgag cgacgtgcgc accgcgtccg tgcgcccgag acgcttgccc
     3181 gtacgcacgt ccgtgacgta gaaggcgcgc gggagcttga cggtgatctc ctggcgcgcg
     3241 acctgcccca gcccggcgtc gttgtagacg gtgacgccgt cctgcccgac gacgcccgcg
     3301 acgccgacgt tctctttgac gaccgccaga atctccgagt cgccgaagcg gtagcggacg
     3361 acctgcgcct gcgccagccg cttgccgtcc gccgtcagca cctcgacggc gggacggacg
     3421 cccgcgcgtt cgagcaggcg cgcgagcagc gcgcggtgtc ccgcgccgcc cgacttctcg
     3481 ccgcgcagct tcgggtagcg gtcgaagagc gcgttgaggt agaacgtcca accctgaccc
     3541 acctgccgcg cgacgaccgc gtccgcgccg ccgacgcgcg ccagcgcccg cccgcccgcc
     3601 gccttcacgt ccgactcggc cgccgccaag tccgccagct cgtcggcctt caagccccac
     3661 ccggcgcctt cagccgtgac ctcggccgcc ccggcgacgc gcgtgagcgc gcgcttgctc
     3721 gactcgggcg cggagatgcc gaacagctcg ttcagcacgc ccctcggctg ccacgcgcag
     3781 tgctcgtcca acagccccgc ggcggcgtcg gcgatgacga cgccccccgt gcgcgcgaac
     3841 tcctcgacgg ccttcgcctc gcgggcagag agcgccacgg agagcgggag gatgaggacg
     3901 cgatacttgc ccgcggagag cgcgccgcct tcgatctgct cggaggcgac gaagtcgggc
     3961 tgaagcccga ggtctttgac ggcgcgcacc cagccgtcgc ggtcggcggg gaagttggcg
     4021 gcagctttct cgggggcttt gtcggcgccg ccgtcgaagt tgcggtggta gccgaggatg
     4081 ctcgcgccgt ggacggaggg catcgagtag tggatggcga tcccgtcccc gagccgctcg
     4141 gactccatca ggagcttgcc gacgccctcg aacctgagcg agcggaaggc cagccccatg
     4201 tcgcgcgcgg agttcgagtg ggtcaggtcg gggtcgagga acgagtacat ccagaagatg
     4261 ttcgggtgca gcacgttgtt gaccgccgcg ttccagatgg cgttctgtac gccgatgccg
     4321 ctcgagccgt accccgtcca gaagccgatc atcgagtcgg gcttggcgaa cgagcggtgc
     4381 atgtcccact ggttgccgcc ctcgtaagag aggaagtcgt cgatgacctg atcgagcctg
     4441 taccagtcgg cgccgttgta ggcgttggtc tcctgcgtgc ccgagagcgc gatgcgcgcc
     4501 tcggggtcgc cctcgacgac cgattcgcgc acccgcgcgt aggcgcgcgc gaaggtcgcc
     4561 tccatgaagg tgcggtggtc ggcccacggc gcgaagttgc cggacttgcg cgcctgctcg
     4621 gtggtcgagg gcagaaccgc atcccagtca cggaagtcgg agcgccacac gcggttgagc
     4681 gcgtcgagcg tcctgtactc gcctttgagc cattcgcgga agtcttcgag cgcctgcggg
     4741 ctccagtcga aatcgactgg gtcgccgtag gaggtgagcg atccctcgtc gccgacgaag
     4801 tagtagtcca tgttaaagcg cgccttgttg cgggccgtgg cgcgcgtgat gccggcgagc
     4861 gcgtccatga aggtgcggtc gttgaaggag ggcgtgcggc gcaggaactt cgtgtcgccg
     4921 gtgcgcttgt acagctcctt cttcgtcagg tagccgagcg cgtcgaagtc gccggtgcgc
     4981 ttgtaatctt cgatggcgcg ctccagcccc tcgggcgtcg tggggccgcg gtcgtaccag
     5041 tagacgccga aggtgccgcg cgggacgttc aaggagttgt ccacatcgcc gccgctccac
     5101 gtgaagcccg tgtcggcggc gacggcccgc acctgccgca tctgcgcgtc ctgtaggtag
     5161 tgcttcgtgc cgccgaatgc gacaagcgcg gtgtactcgc gcgcgcgccg cttggcctgt
     5221 acgacgagca cgggcttggc gcggagctgg tctacgacag cgccgcccgc gtccgccagc
     5281 tccgccgtaa cgaaagcctg cttgccgaca aagtcctgga gcgcgtaaga gaacgtccgc
     5341 tcgccgcgcg ccggagcttc gaggacggtg agcagtcggc ccaaatcgtc cgacaccttc
     5401 agccgcatcc ttaagccttc gaggttcccc gtcgcgcgca ccaccgcgct cagcgtctcg
     5461 ccgcgcttgt agacatccac cgccgacttc gcctgcgtga ccatcgcgcg cttcggggcg
     5521 tggcggacgg cggatcccca gttgagcgtg gcgccggtgc gcgcgtcgcg caggatgagg
     5581 tcgtaaatct gcctgccgcc ggccagcccc ccaccatccg cagcagcagc cggggccagc
     5641 tcgaacgagt tccgaccggc ggccagctcc ttcgtcacct tgtgcgagcc gagacgttgg
     5701 ccgaactcgc tcttgcccga gacctcgatc tcgacgcggc gcgcggcgtc ggacgtgagc
     5761 gcgagcttga cggacggacg gcccgcggcg tcggcggcga gcgagtcgat gcgcacgccc
     5821 gcgtcgcggc ccgccgccca gaggacggcg cgggagagga gtgagtactg gtactcccag
     5881 tagtcccagt aggtcttcgt ctcgaagggg ttgacgctct gcggcgtgaa gccctcctcg
     5941 acgtaggcga aggcgacgac gcgccccttg ccgtaagtct tgacggcggc gaccgggtgc
     6001 tcgcccgacc tgatgatgac gtcgcccgcg gccttgtact tgtagaaaga gccgccggcc
     6061 tgcccttcgg ggaggagttc gagcggcagc ccctcggtga tgaagtgcgg gcgggcggcc
     6121 tcccaacggc ccctggtcac ggcgtcctga ttgacctcag ggtagccgcg ctcgttgacc
     6181 gtatcgtcgg ggacgccgac gaggggcgac agctcccaga tgcgcgcgtc cgcctccgcc
     6241 tcgtcgcctt tgaaggggtg ccccttcacg tcgccgagga aggggtggag caggacgagg
     6301 ccggcgccgt cgcggacgcg gcgcagcacg gcgtcgcgcg ccgggcgcgt catgcgcgac
     6361 cagccgttca gaccggggat gaccatcacc tcgaacgggg cggggccggt cagatctttc
     6421 tccacgtagc catagacgat ccggaagtcg tcgcggtcgc cgcggtggac gtggtcgtag
     6481 aaatcgccga tgccccagca gttgatgtcc caactccggt cgatggagac ggtcgtcggg
     6541 gcgagctgta ggcgttgcat cagctcgacc atgtcgcgtc cgtactggat ggaggggatg
     6601 aagaagccct tgacggggcc gccctgaagc ttcgtcgccc acgggacgtg cggcgtcgcg
     6661 acctcggtcg agtaggtgat gtcgttcttg tggaagctgc tcccgccgac cccggtctgg
     6721 cgggcgcggg tgtcggggac gagcgaggcg agcacgagag cggacagtgc taaggataaa
     6781 agctttctca ttaagtttgc tttgccgtcg gacatggttg aagtttgtgc gtaactcgcc
     6841 gcggagcggc gatttaggca cacgcttcgg atagttgtta tgacccgcgg gggcagccgt
     6901 gtcccggggg ctttagcctc gaccttgacc tgttaactcc tcggcgatac ggaagcagac
     6961 gcgcgacgag gggagaacgt ctgcgacggt cggggcgggg cgtctccctt cgcgtagcgc
     7021 gccgacgaac tccaagacct cttcgtaggc gccgctgcgc aggtcttccg gcgccgtctc
     7081 gtcggcgaac tcttccgttt cgaggcgtcc gccgcgccag cagtgcagtg agcgctgcgg
     7141 cccggagccg gcgacgacgc gcgcgcgaaa gccttcgccg aacagctcgt acgactcttc
     7201 gatgacgccg gcggtcggaa gcacctcaag ctgcccgcgc gcgcccccct cgaagtcgag
     7261 cgagacgaga taccagttgg ccgcaagccc ttcggggcgg tgcagcgtcg cgcggtagtc
     7321 ctttatctcg cccgcgacgt ggcgcagggc gtcgagcgcg tggagcgccg tctcccagat
     7381 gaaggccggt tcgtcgcgcc gttggcgcac catcgtcgca cgggcgtaaa ggagtccgcc
     7441 cgcctcgcgc gcccaggccg aggcgcggtt gagatagggc atgaatcggc ggttgaccga
     7501 gaccatgtgc ggcgtgcccg tgcgtcccgc gacctcggcc agccgctcga cctcggcgag
     7561 agacgtgccg agcggcttct cgatgacgca cgggatgccg cgctccagca ccttcgcggc
     7621 cacctcgccg atgcgatcga ccggcatgac ggtgacgcag ccgtcgagtc gctcagccgc
     7681 gagcagttca tccagactcg cgtaggcgcg cgcgaagccg aactcgcgac agaacctttc
     7741 ggcgcgcgcg cggtcgaggt cgcaggcggc ggccagctcg atcccgcccg gtcgcgcggc
     7801 ggcgtagcgc cggagcggcg ccgcatggct cgactcggaa tgttctccgc acccgacgag
     7861 tgccagccga agcgtcacgc gccgtctcct cctttgccgt acctacccgc gtccgtcgag
     7921 cgattcgacc gcggcttgcg gcacgagccg ctcgacctgc cgcgcgtcga tcatgccggg
     7981 ggcgtcggcc aaacagttcg ccgtcccgca ggcggcggcg aacctcacga actctcgccc
     8041 cgagagtccg cgccggtgcg cgacggcgaa gcctgcgagc gacgcgtcgc cgcaccccac
     8101 tgccgagcgc gcctcgacgc gcggcggacg ggcgacgaaa ggcgccgagt cgcgcgtcgc
     8161 ctgccagaac atgccctcgg ctccgagcga gatggcgacc ccgcgcgcgc ccgcctcgaa
     8221 gagaagacgg gcggcttccg ccgccgcgcg cgcgccgtcg aggcgcacgc ccgtcagcgc
     8281 ctcggcctcg tcgcggttcg gcttgacgag gtcgggcgac tcgctgagcg ccgcgcgcag
     8341 cgcctcgccg ctcgtgtcga gcagcgcacg gcaaccgtgt gcgtgcacga ggcggacgag
     8401 ctgcgcgtag aagtccgcgg gcgcgcccgg cgggaggctg cccgagagag cgacctgcgc
     8461 cgcccccctg tactcggtga agaggtcgcg gcagagcgag agcatgcgct cgacttcacc
     8521 ctcgctgacg tgcccgcccg gctccagcac ctccgtgacc gtgccgtcgg cctcgacgat
     8581 ctccatgttc atgcgcgtct cggcggcggt cttcaccacc gccatgggga tgccgagcga
     8641 gacaagcccg cgctcgcact cttcgcccgt cgagccgccg aggaagccta cccacgtcac
     8701 acgctcgccg agcgcggcag ccgccatcgc gacgtgcgcg gccttgccgc ccgtcatggc
     8761 gcacacggag gtcgcgcggt tgaccgcgcc ggcgtggagc ctgcccagac gcaggcggcg
     8821 gtcgatggcc gggttggcac tcacgcagat gatcatctcg tcaccctccg ggcgcgcgcg
     8881 gggcgcccgt cactgctgcc ttcagccgcc caggagcctg agcgcgttgt cgcggtagac
     8941 gcggcgcagc acctcgtcgg gcaggcggag accgtaaatc tgccagcgcc cctgccgcga
     9001 cgcgtgcgag gggtagtcga aatactcgtc cgccgtctcc agaaagcgga agtagagccg
     9061 gtacatctcc tcctcgggga cgaggtccgt gccgaagagg atgcgttcgc tgtagttgtc
     9121 gaagaactcg cgcgccgcgc gcggctggcg accgagttcg gaggcgcgcg cgctgatgtc
     9181 cacggagaag ttcgggtgcg catcgagcca gccggccacc tccgccaggc attcggggcg
     9241 ctcggcgacg tgcgcgccga cgaatcgcgt gcgcgggtgg cgcgcgagga cgcggttgcg
     9301 ctgcgcgagc agctcggcct tcgtgaagtg cgagccgtgg aacgaccagt cggggtgcgc
     9361 gctcagctcc tcgtagcgtt cgttgaagcg gtcgatgggt tggaagaatg cgtccgggtc
     9421 ggcggtgtgg aacatgacga agacgcccaa ctcgcccgcg cgctcgaaga gtggggcgag
     9481 ccgctcgtcg tccacgcgca gcagcgagcc ttccgggccg cgcaggctga gccccaaatc
     9541 cttccagacc ttcaggccgc gcgccccctt cgcgaccatg cgttcgagcc gcccgacgct
     9601 gcgcgcgaag aagccgggct cgcccacgtc ccgccagtcc atccacgcga ccgtcgagaa
     9661 gcggtcgggc gcggcgcggc ggaagcgctc caacacgcgg acggcctcgt cgcccacgcg
     9721 catcgtgatg ttgacgcagc gctcgatgtt gcaggcgtcc aacacgcgca gcacctcgcg
     9781 tggctcctgc gcgtcgaggt ggttgtggaa gtcgatggcc gggtacttcg cgcgctgaac
     9841 gtcgtgcgcg gggacgacca gcgacgcgcg cggcgcgaag tccgtgaggc gtagctcggt
     9901 ctcggccttc gcgctcacca tcgcgatgaa tttgtcctcc ggctcgtcct tcaactcttc
     9961 gcccgccccc tcgaatcgcc cgtcaagtcc cgcccgtccg gctgctcagt cctgccgcgc
    10021 gccgccctcc ccgtgttcct cctccgtcgg ctcgccctcc tcgcccgtga tctcgctgcg
    10081 tgaagaccag aggcggttga tgacgcggag cagtaccaaa ccgctcacga cgaagacggc
    10141 gagcagcgcc aacccgtaga cgtaccggcc gtagatgaac tggccgaccg tgaagaggga
    10201 agaccagatc atcgcgcagc cggccgccca cccgagcatc gccatcggga tgttgtcggc
    10261 cctctccacg gcgcccttct cgattcccgc ctcgcgcctg acgctctccc agccggggcc
    10321 ggacgggtga accttgcggt agaactcgac cagcgtccgg cggtcggtct gcggcgcgag
    10381 gtaggcggcc agcacccagc agacggtcgt aaagccgacg gtcatcagga gccgctgcga
    10441 cgtggtggcc gagtagaccc cctgcttctc cagcacgagg aaggcgaccg agatgaggaa
    10501 cgagctgacc atcgccacca cctcgctcca cgcgttgatg cgccaccaga accagcgcag
    10561 gaggtagagc agccccgtgc ccgccccgac ctgaaggatg aggtcgaagg tgtccttcgc
    10621 cgtgtcgagg acgaagacga gcgccgaaga gacgacgaac agcaggacgg tcgccacgcg
    10681 gcccgcgagg acgtagtgct tctccgtggc gtcggtgcgc acgaagcggc ggtagaagtc
    10741 gtgcacgagg taggaggcgc cccagttcag gtgcgtcagg atggtcgagg agttggcggc
    10801 cgcgagcccg ccgaccacga ggccgacgaa gcccgccggg aggaacttga gcatcgccgg
    10861 gtaggcgatg tcgtggccga gcagcgccgg gtcgaggtcg gggaacgcct tctggatgtc
    10921 cgagagctgc gggtagacga cgagcgaggc gagcgccacc aggatccacg gccagggccg
    10981 cagcacgtag tgcgcgacgt tgaagaagag gaccgcgccg agcgcgtcct tctccgactt
    11041 cgaggccagc atgcgctgcg cgatgtagct gccgccgccc ggctcggcgc cgggatacca
    11101 gaccgaccac cactgcacgg cgatgggcag gacgaagacg gcggcggcga cgtcccagtt
    11161 gttcgtgaag tccggcagca cgtccaggta cctgatcccg cccgggccgc tcatcgccga
    11221 gaccttctcg acgagcccgc cgatgccgcc gacctccggg atgtagacgg cgaagtaggc
    11281 cgccgccacc accgccgtca tcatgatgaa gaacttcacc atgtcgatca ccagcacgcc
    11341 ccacaggccg gagtgtgcgg cgaagacgac gttgaggagg ccgacggcca gcagcgtctg
    11401 ccagcgcggg agcccgaaca tgatggccgc gatcttgcac gcggccaggt tgaccgtcgc
    11461 catgatcagg cagttgaaga agaggccgag gtagacggcg cggaagccgc gcacggcgct
    11521 ggcggccctg cccgagtagc gtatctcgta gaactcgagg tccgtgagca cgcccgagcg
    11581 ccgccacagg cgcgcgtaga agaagaccgt cgccacgccc gtcagcacga acgcccacca
    11641 gacccagttg cccgccacgc cctgccgccg gacgatgtcg gtcacgaggt tcggcgtgtc
    11701 actgctgaag gtggtcgcca ccatcgagat gccggcgagc caccacggga ccgaacgccc
    11761 cgaggcgaag aactccgagg tgtccttccc ggccctcctc ccgaagagga gcgccgggag
    11821 gaagcagatc gcggtgacga acagcgcgac gaaccagtcg agtgcgccaa aattcatgtg
    11881 acgatccttt caacccgttt cgagtgtcgt cccgtctccg gggcgggcgc cgcgcggcgc
    11941 gccggtcaca gcagggagca gaccctgatc ttggctgacg aaatctcgcg gacggcctcg
    12001 atggccggcg cgaaggtctt ccgcaggtcg atctcgtcgc tcgcggagag cgactgcttg
    12061 acggccagcg tgaaagcgtt cttcagctcc gtcccgaagt tgatcttgcg gatgccgcac
    12121 gagacggcgc ggcgcatcag ctcgtcgggc acgcccgagc cgccgtgcag cacgagcgcc
    12181 gccgcgtagc ccacggcgtc gcgaatctgc tccagccgct cgaagtcgag ccgcacctcg
    12241 cccttgtaga agccgtgagc ggtgccgacg gcgacggcga gcgcgtccac gcccgtctcc
    12301 tcgacgaatc gcgctgccgt atcggggcgc gtgtagaagc gcgaaatgtc tccgccctcc
    12361 tcgttgtgcg cgacgtgccc cagctcgccc tcgaccgaga cgcccgcggc gtgcgccagc
    12421 tcgaccacgc ggcgcgtcag cccgacgttc tcgtcgaagg ggagcttcga gccgtcgatc
    12481 atgacggagg tgtagccggc ctcgacgcag cgcgcggcca gctcgtagct cccgccgtgg
    12541 tcgaggtgga gcgcgaccgg tcgctcgatc tcctcggcgg cggcgcgcgc catcgcgacg
    12601 acgagtttga tgccgaggta gtcgatggtg gactccgtgg tctggaggat gacggaggcg
    12661 ccgcactcgt tcgcggcctt gatgtgcgcg cgcagcgtct cggcgttgta gaagttgagc
    12721 gcggcgacgg cccgcccctc gcggcgcgcc tcttcgagga gccggcggct cggcaccggc
    12781 gtgcgccccg ccccccgcgt cgttgctgtc tgttcgctcg gactcgtcat tggtgtagct
    12841 cgtcagtctg caagttcgtt cgaacggtct accgtcccgc ctgcgcgcgc aggaaacgtt
    12901 ccagcgccgc gcggtcgggc tggttggccg tgccgcccgc cttcagcgtc gagagcgcgc
    12961 cgcaggcgtt gccgcgccgc aggcattctt cgaggcccag cccctcaagg tgcgccgaga
    13021 cgaagccggc ggcgaaggtg tcgccggcgc cggtcgtgtc cagcgcgtcc accctgaacc
    13081 ccggctccga ggcgacgcgt ccgtccctga tcgccacggc gcccttcgcc cccagcttga
    13141 cgaccgcgca ggggacgcgc cgggccagcg ccctgagcgc gccccgcgtg tcgcgcgccc
    13201 gcgtcagttg cagcgcctcg tccctgttca ggaagaagat gtccgtgtgt tcgagcacgt
    13261 tccagatgtc ttcgccccac gagctggtcg ggtcggaatt cgggtcgaag gaggaggtca
    13321 gccccttcgc gcgcgcctct cggaagatac gcgcgaagga ggggcgcaag ccctcctgca
    13381 aaaagtacga ggtcaggtgg aggtggctct tgcccttcag caaggacatc cgcacgtcct
    13441 cgtacctgag cgaggagatg gcgccgaggt acgtgacgag cgcgcgatcc ctgcgcgtgc
    13501 tgagcgcgag cgtgacgccg gtcttctccg aggggtcgcg cagaacgtgg cgggtcggga
    13561 tgccgttggc gcgcagggct tcgaggcaga ggtcgccgaa atcatcccgt ccaactcggc
    13621 tgacgaaggt cacctcgtgg ccgagctttg ccaccccgca ggcgaagatg gccgaggcgc
    13681 tgcccagggt caactggaag tcctcggcga tgatctcaag cccgagtttc ggctcctgcg
    13741 cgagacccgt cgcgacggcg tccacgttca actcgccgat cacgacaacg ccgctacctg
    13801 cctcttctct catcagattt gactatttgg ttccgactac ttacgataac tttcgacggg
    13861 agccggattg taatacacat cgaaagtgaa agcaaaggta ttcggacgca ctttgaaggg
    13921 gtgctgttgt tccggcacga gggcgggaag ggggcgagga cgatggacga attgagcagg
    13981 ctgctggcgc tgtccgaaga ggagagggcg gagcagggtt tggttcacac cccgcgcgag
    14041 atttaccagc agcccgacac ctggcggcgc acctacgagg acttttcagc ggtcgccggt
    14101 gaagcgcagg acttcctcgc gcgggccggg ctgtcggggg gcgtggcgcc gaccgtctac
    14161 ctggtggggg ccggcacctc cgactacatc gggcgggctt tggcctcgct cctgcggcgt
    14221 gagtggcgct gcgaggttca ggccgtcccg agcaccgacc tgctcacgga catggacgag
    14281 cacgtcatcc ccggacgccc gtacctgtgg gtctccttct cgcgctcggg cgacagctcc
    14341 gagggcgtcg ccgtcatcga ggcggcgctc gaacgccacc cgtcagtccg gcacctgatc
    14401 gtcacctgta acggtggggg gaggatggcg cgcgacttcc gcggacgcga gaacgtcttc
    14461 tgcctcgtcc tcgacgacgc ggtgaacgac cgcgggctgg ccatgaccag ctccttcacg
    14521 aacatggtgg tcgccggcca gtgcctcgcg cacgtccgag acctcggggc ttacgggcgg
    14581 gtcgtcgaag agctggccgc agccgcagag aggttcacgg gggcggcggc ggacgcggcg
    14641 gcgaggcttg cgtccgagcg gcacacgaag ttctgcttcc ttgggacggg ggcgctgcgc
    14701 gcggtcgcgg tcgagtcggc gctgaaggtt ctggagctga ccgccgggcg ggtctacacc
    14761 tggtcggagt cgtacctcgg ggtcagacac gggcccctct cggcagtgga cgacgagacg
    14821 ttggtcgtgg ggttcctttc gggcgacggg cggcgcaggg cctacgagct cgacctgttg
    14881 cgtgaggtca gggccaaggg tctcgcgggc aagatcgtcg ccgtgtggcc gtgcgacgcg
    14941 tcgcaggcgg gcgggttcga cgaaacactc tcgctcgggc tggccgccgg gttcgaagac
    15001 ctccacaggc cgccgctgga cgtgatcttc ggacagctct tcggtctctt caactcactg
    15061 gggcagggcc tcaagcccga cacaccaagc ccgaagggag ccatcacacg ggtcgtcgag
    15121 cgcgtgcgga ttcactagcc gtccgcgcgc aaggtgctca ggtgagtatc agttcgacgc
    15181 ccagctcgcg cagccccgcg cgcatcgcgt cgggcagccc gtggtcggtg acgacgcgcg
    15241 cgaccttgga cagcgacacg atccgcgaga gactgcgcct gccgaacttg ctcgaatcgg
    15301 cgacgagtat cgtctcgcgc gcggcgcgca ccatcgcctg gttgacgtgc gactcgtcga
    15361 ggttgggcgt gctcaggccg aactccagat cgacggcgtc cacggccagg aagagcttgt
    15421 ccaccgagag ttgttcgagc atctcctcgg cgaactggcc gacgagcgag ccggagttct
    15481 gccgcaggag cccgccgagg agtatcacct cgaccccgcc ggcgcccgtc agctccgccg
    15541 ccacgttgag ggcgttggtg atgaccttga ggccgcgcct ctccttgatg tgccgggcta
    15601 cctgctgcgt ggtcgtcccg gagtcgatga tgatcgactc gccgtcggcg acaagcgccg
    15661 ccgccgccgc cccgatgcgc cgcttctcgt cggcgtgcag cgcggccttg acctggagcg
    15721 aggggtcggc gctgaccgac tgggcgcgca ccgcgccgcc gtgcgcgcgg cgcaccagcc
    15781 cgcgcgcgtg cagctccttg aggtctttgc ggatcgtgac ttcggaggcg cggaggcggc
    15841 ggctgaggtc gccgacctcg accttcccgt gctcctcgat gagttcgagg attctggcct
    15901 gtctctccag gttcagcatg ccggtcgtgt gaccctcccg ccggcatcaa gttataacag
    15961 accggcgtcc cgtgaagtca acaaagccca ggccggttca gcatggcgtg gcgcgcggcg
    16021 ccgagcgcgc cggccacgcc gccgagcgca gccgggacga cgcgcacgcg gtggccgttg
    16081 ggtcgccact ccatcaaacc gaggtagcgt tcgagcgggt cgaagagcgc agccccggcc
    16141 tgcgcgatgc cgccgacgat gacgaaaacc tcggggacga gcacgttgat catcgaggtg
    16201 acggcgcagg cgagctggta gacggccctc agccagacgc gcgtggcgtt cacgtcgccc
    16261 gcgaggtgcg cggcgaccag cgcctcggtc gagtcgaaga ggccgcggga gcgtttcgcc
    16321 agcgtgcggt tgccgaccgc ctcttcgagc gtgccgggga tgccgaggct gtccggctcg
    16381 ccgtccacgt ccaggcagat atgcccgatg tggccgccgc gccccaggtg tcccgtgagc
    16441 agacgcccgc cggtgagcac cgcgccgccg acgcccgtgc cgagcgtcag catcaccgcg
    16501 tcccgcgcgc cggcagccgc acccttccac gcctcggcga gcagcgccgc ctgcccgtcg
    16561 ttgatgagcg ccggcgccct cccgtcgccg agatattcga tccagtcgaa gccttggagc
    16621 gattcgaggt gaccgctcac ggaggccatc gagcgtccgt cgcgcgccgc catgcccggc
    16681 gacgcgatgc cgacgcacgc agccgccccg ccgaggcgtg cctccagagc ctctatcagc
    16741 tcgcgcacgc ccgcggccca ggccgcccgg ccatcgtcgc gcgtctcggc caacgactcg
    16801 gccagcactt cgcccgaggg gcggacaacc gccgccttga tattcgtccc gccgaggtcg
    16861 atgccgatcg cgtagtccat gaagtcagtc cgtcgagacc gcccgcccgg cgccctccga
    16921 cgggtccgcg cgaatactct aacgcacggc ttagatgcgc cgcaccagcc cctcttcggt
    16981 cgcgcgcgtg ttgcgcacga gcaggagcac gagcgccatc ccgacgagcg ggatgaggcc
    17041 ggcgacgacg atgatcgggt cgaaggccgc cgtgcccatg ccggcgcgcg cgtccgagac
    17101 gtagccgacg aggaggaagg cgatgatggt gccaagcccg gcgcccgtcc cgctcagccc
    17161 gctcaccgac gccaccgact cgctgtggaa gaggtcggaa gggagcacgt tggcgatggt
    17221 cgtgaacgag gcgtaagaga aggtggcgaa gccgaagagc agggcgatgg cgaggaggtt
    17281 cgtcgtgaag acggtcggga tgagggcggt cacgcctagc ccgccgaaga cgacgagcgc
    17341 cttgcgcgcc gcgccgaccg accacccgcg cttgatgagg tggcccgaga gccagccccc
    17401 gaagaagttg ccgaggtcgg cggcgatgaa cggaatccag aaggcgaaga gtccttcttc
    17461 gagcctgatg cccttggcga cgagatagat ggggaaccag tcggtgacga agaaccagac
    17521 cgggtcggtc agagtccgcg cggagatggt gccccaggtc tgcgggagcc ggaggagctg
    17581 cccccagcgc gggcgcaccc tctcaacgcc ctccggctcg tcctcctgct tgtcgcgcag
    17641 gatcatctcg cgctcctccg cggatatgcg cgggtgctcc tcggggcggt ggtagagcca
    17701 gcgccacgcg gcgagccaca ggaagccgag cacgcccggc acgatgaagg ccggtcgcca
    17761 gccccacttg aaatagatgc cgaggacgag gtagggcgcg atggccgcgc cgatggacga
    17821 gccgctgtcg aagagtgccg tggcgaggcc gcgctcgcgc ttggggaacc actccgagac
    17881 ggctttggtc gccgccggcc agttggccga ctcgcccacg ccgagcagga agcggaaggc
    17941 cgcgaagctg tagaagccgc cggccagcga cgtgagcatc gagacgacgg agtaccagac
    18001 gaccgagagc gtcagccccc gccgcgtgcc cagcttatcg acgaggcgtc cgcagaccgt
    18061 ctgcccgatg gagtaggcga tgcggaacga gatgacgagg taggcatagt cggtgttcgt
    18121 ccagccgtag tcctgcttga ggaagggggc gaggttcgag agcgtctggc ggtcgatgta
    18181 gttgatgacg gtcgaggcga agagcaggcc gccgatccac caccgcaggc gtttgatcgg
    18241 cgcgcggcgg cccgcgccgg gttgttcaga caatgccatg cttatcgg
//
LOCUS       DBNGOH010000995         5356 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4000070, whole genome shotgun sequence.
ACCESSION   DBNGOH010000995 DBNGOH010000000
VERSION     DBNGOH010000995.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5356)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5356)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5356
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4000070"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..290
                     /locus_tag="AC159P_19395"
     CDS             <1..290
                     /locus_tag="AC159P_19395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007948769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HIR3697660.1"
                     /translation="VIPMFSYPAEIRRAVYTTNTIESLNMTLRKVSKNRSLFPNDEAV
                     FKLMYLALHNIDKRWTMPIPNRSGALNQFAILFDGRVPMDGLNQNSLTQTA"
     gene            complement(498..1220)
                     /locus_tag="AC159P_19400"
     CDS             complement(498..1220)
                     /locus_tag="AC159P_19400"
                     /inference="COORDINATES: protein motif:HMM:NF012415.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HIR3697661.1"
                     /translation="MKRTPDLFVTIPLCALALLLAAPAAPCARQLAAVAPGTFAALEQ
                     EIAREINLARTRPAEYAPFLEQLRPLYTGREFRRPGGPALLTEEGVGALDEAVGHLRA
                     ARPAPPLEVSEGMCAGAAALVKDQAGSERTGHKGADGSFCEQRVGRFGSWAAPIGENL
                     SYGAETARERVIALLVDDGFANRSHRRRLLDPSFKVVGVACGGHRLGAVCVITLAGGF
                     TRKPAAGQRPAPARAPAGARKY"
     gene            1471..1911
                     /locus_tag="AC159P_19405"
     CDS             1471..1911
                     /locus_tag="AC159P_19405"
                     /inference="COORDINATES: protein motif:HMM:NF013540.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR3697662.1"
                     /translation="MRYEKGLSQRDVEVRSGGGISKGYIGQIETREVLGHSVTPQKLQ
                     ALAAGLGISEDELFAVARGVSSAGDLSLEEVRLLHFYRSIPPGRRADAIGYLEMLFKM
                     YGANGQPEGVGKRGPQPGKVYDAVAGIPKSVKRARKSSSKKPKR"
     gene            1996..2733
                     /locus_tag="AC159P_19410"
     CDS             1996..2733
                     /locus_tag="AC159P_19410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697663.1"
                     /translation="MSSQPTNAAARLYDILSEARSSPEGSVRDRWKAVLGDKHMNDAE
                     LVIGMVAVHTLFDEVEDGLRGAVPNLNENLYIGPLSKLRQAVYFGNLDTNWSSHLAKI
                     GDADMRALAFAADRLSEPPPEPTVDTDSLAEILRAVNDLYDEVLRSDTPRPLRRLLLE
                     QLAEIQRAVHLYRVQGVAPIRAALAKTLGVIYANEDVIKKDADKGEVKSFGAVLKRVD
                     EVISLATKAKPLLGPAVEFIRPLLPGG"
     gene            2932..3360
                     /locus_tag="AC159P_19415"
     CDS             2932..3360
                     /locus_tag="AC159P_19415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007290999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family protein"
                     /protein_id="HIR3697664.1"
                     /translation="MSDITAVYKAICSKPLSRPLVGFRVSAGFPSPAEDYIEGSLDLT
                     KYLVRHPVATFYIRVRGDSMVGANIHPGNLLVVDRAAETFDGHIVVARIGDELCIKRL
                     HIVEGCVWLCAENPEYDPIEITGEMDFEIWGRVMHSVQSF"
     gene            3416..3811
                     /locus_tag="AC159P_19420"
     CDS             3416..3811
                     /locus_tag="AC159P_19420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697665.1"
                     /translation="MITTDHIHILYAVVMLPITHGGLAAIAFGVMRMSGDAVMSTNVA
                     GVITIGVLLLTAGNIPWRITREGWIVIFSIHETLVSVERRLSAAEAYTKAVFDRLRAT
                     EENNDDRHEELLSALRQPGAARTGARPSA"
     gene            3825..5081
                     /locus_tag="AC159P_19425"
     CDS             3825..5081
                     /locus_tag="AC159P_19425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0034061 - DNA polymerase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HIR3697666.1"
                     /translation="MNGHFALVDCNNFYVSCERVFDPRLIGRPVVVLSNNDGCVVARS
                     NEAKALGIRMGVPLFRIQDLVDYHKVAVLSSNYALYGDMSSRVMAVLHDLAPVVEVYS
                     IDEAFLLLDLPQAWLQEFARRARERVYRWTGIPVSVGVAPTKTLAKVAGERAKKSPDG
                     VCDLTDRERADEVLASLPVGDVWGVGRQYAKLLRSRGIETAKQLRDSDERWARKAMTV
                     VGARIVHELRGTSCLALEAAPPAKKSVTCSRSFGQPVETPGELKEAVAYYTQSAAERL
                     RRHRLAAGAVTVFVSTNRFSKSEPQYSNSATVEVAFPTDSTQELLAVTLRAAEKMFRE
                     GFRFKKAGVILSALVPSSPMTARMYGDALWQKSRRLMGAIDEINRKFGKDSIRFAAGG
                     MRRVWKTKAERESPRYTTCWSDVLAV"
BASE COUNT         1010 a         1734 c         1760 g          852 t
ORIGIN      
        1 gggtcatccc gatgttctcg taccctgcgg agatccggcg cgccgtctac acgacgaata
       61 cgatcgagtc tttgaacatg acgttgcgga aggtgagcaa gaaccgctcg ctgttcccga
      121 acgacgaggc ggtgttcaag ctgatgtacc tggccttgca caacatcgac aagcggtgga
      181 caatgccgat cccgaaccgg agcggtgcgc tcaaccagtt cgcgatcctc ttcgacggtc
      241 gagtcccgat ggatgggctc aaccagaact cacttacaca gaccgcttag aacttccgca
      301 cccgacagct cgtcaccaag aaaagaagtg tgcagatagc ggaaagccgg aatcgatcca
      361 tcatagagtg caacgtcggc gggcagatat tacgggccga cgtaagaacg cccgtctgtg
      421 agtccccgcg atttttccca ccctcgatcc gacctcctcc cgagctccaa gtcccgactt
      481 cttcaccgcg gccggtctca atatttgcgt gcgccggccg gggcccgcgc gggggcgggg
      541 cgctgcccgg cggcgggctt gcgggtgaag ccgccggcca gggtgatgac gcagaccgcg
      601 ccgagtcgat ggccgccgca ggcgacgccg acaaccttga aggacgggtc gagcagccgc
      661 cggcggtggc tgcggttggc gaacccgtcg tcgacgagga gcgcgatgac gcgctcgcgc
      721 gccgtctcgg cgccgtagga gaggttctcg ccgatgggcg ccgcccaaga cccgaagcga
      781 ccgacgcgct gctcgcagaa actcccgtct gcccccttgt gccccgtccg ctcggacccg
      841 gcctgatcct tcaccagggc ggccgcgccc gcgcacatcc cttccgagac ttcgagcggc
      901 ggcgccgggc gagccgcgcg caggtgaccg acggcctcgt cgagtgcgcc gaccccctcc
      961 tcggtgagca gcgcggggcc gcccgggcgc cggaactcgc gcccggtgta gaggggccgg
     1021 agctgttcga ggaagggcgc gtactcggcc gggcgcgtcc gcgcgaggtt gatctcgcgg
     1081 gcgatctcct gttcgagcgc ggcgaaggtg cccggcgcga ccgcggctaa ttgccgggcg
     1141 cagggtgcgg cgggtgcggc gagcaggagg gcgagggcgc agagtggaat cgtgacgaag
     1201 aggtcggggg ttcgcttcat tggggcacct caaccattgt tgggaaagaa gaatgaactt
     1261 cggcggcgtg cagagttgta tgctaaacgt gccggcgcgt caagggaatt agacgagacg
     1321 acaggagaac gttgtccgct tcggccagac accggtatcg ctactatatc taccttattg
     1381 gtagatatat catgcgggcg atatattagc cttaccgccg tgaatagtag gcgtgagcaa
     1441 atcgaggacc tagccgatta cgtgaggcgc gtgcgctacg aaaaaggcct ctcccagcgg
     1501 gacgtagagg tcagaagcgg gggcgggatc tcgaagggct acatcgggca gatcgagacc
     1561 cgggaggtcc tcggccattc cgtcaccccg cagaagttgc aggcgctcgc cgccggcctc
     1621 ggcatctccg aggacgaact cttcgccgtg gcacgcgggg tgagctcagc cggcgacctg
     1681 agtctcgagg aagtgcgcct cctccacttc taccgcagca tcccgcccgg gcggcgcgcc
     1741 gacgccatcg gctacctcga aatgctcttc aagatgtacg gggccaacgg gcagccggaa
     1801 ggcgtgggca agagggggcc gcagcccggc aaggtctacg acgccgtggc cggcatcccg
     1861 aagtccgtca agagggcgag gaagtcgtcg tctaagaagc ccaaaagata atcgcaccgc
     1921 cccgcgcccg ccctcccgac gggcgccaac ttttaacctc atcactcccg gacgaggtct
     1981 cgaccgcccg gctttatgag cagccagccg accaacgccg ccgcgagact ctatgacatc
     2041 ctctccgaag cgaggtcgtc accggagggg tcggtccgcg accgctggaa ggcggtccta
     2101 ggcgacaagc acatgaacga cgccgagttg gtgatcggca tggtggcggt gcacaccctt
     2161 ttcgacgagg tggaggacgg gctgcgcggc gccgtcccga acctgaacga gaatctctac
     2221 atcggcccgc tgtccaagct caggcaggcg gtctacttcg gcaacctcga caccaactgg
     2281 tcatcacact tggctaaaat cggcgacgcg gacatgcggg cgctcgcctt cgccgcggac
     2341 aggctctcgg agccgccccc cgagccgacg gtcgacacgg acagtctcgc cgagatcctg
     2401 cgggcggtga atgatcttta cgacgaggtg ctccggtccg acacgcccag gccgctgagg
     2461 cggctactcc ttgaacaact ggcagaaatc cagagggcgg tccacctgta ccgcgtccaa
     2521 ggcgtggccc cgatccgcgc ggcgctggct aaaacgctcg gggtcatcta cgcgaacgaa
     2581 gacgtgatca agaaggacgc ggataaaggg gaggttaaaa gcttcggggc ggtgctgaag
     2641 agggtcgacg aagtcatctc gctcgcgacg aaagccaagc cgctcctcgg ccccgcggtc
     2701 gaattcattc ggcccctctt acccggcgga tgacacgtcg ccgaacttct cgctgacgcg
     2761 cacgcggatt ttccgcctcc acgagatgtt gatcgagacc gggcccaggg cgacgtagaa
     2821 gtgacccggt aggtagctcc aaaagaagga tcgagacttc ggcatggcgg cctccgccgc
     2881 cgatactatc acactcgcga caggcgaacc ggggaaggag aaatacaagc cgtgtccgac
     2941 atcaccgccg tctacaaggc gatctgctcg aagccgctct cgaggccgct cgtcgggttc
     3001 cgcgtctccg ccggcttccc cagcccggcc gaagactaca tcgaggggtc gctcgacctg
     3061 acgaagtacc tcgtcaggca cccggtcgca acgttctaca tccgcgtgag gggcgactcg
     3121 atggtcgggg ccaacatcca ccccggcaat ctcctcgtcg tcgaccgggc cgccgaaacc
     3181 ttcgacgggc acatcgtcgt cgccaggatc ggcgacgagc tgtgcatcaa gcggctgcac
     3241 atcgtagagg ggtgcgtctg gctctgcgcc gagaacccgg agtacgaccc catcgagatc
     3301 acgggggaga tggacttcga gatctggggc cgcgtgatgc acagcgtgca gtctttctaa
     3361 agccgcgaaa ggagcgccgc atgtcgggag gaggaggtta ctcctcgtcc aagtcatgat
     3421 aaccactgac cacatccaca tcttatacgc agtggtgatg ctgccgatca cgcacggggg
     3481 tctcgccgcg atcgccttcg gagtgatgcg gatgtcaggc gacgcggtca tgtccactaa
     3541 cgtcgccggc gtgattacca tcggcgttct cctgctcacc gccggcaaca tcccctggcg
     3601 cattacccgc gagggctgga tcgtcatctt cagcatccac gagacgctcg tttcggtcga
     3661 gcggcgcctg agcgcagccg aggcgtacac gaaggccgta ttcgaccggc tccgcgccac
     3721 ggaggagaac aacgacgacc ggcacgaaga attgctgtcc gccctgcggc agcccggcgc
     3781 ggcgcggact ggcgcgcgac cgtcggcttg agggggttta tgtcgtgaac ggccatttcg
     3841 cgctcgtcga ctgcaacaac ttctacgtct cctgcgagcg ggtcttcgac ccccggctca
     3901 tcggcaggcc ggtcgtcgtc ctctcgaaca acgacggctg cgtcgtcgcc cgctcgaacg
     3961 aggcgaaggc cctcggcatc aggatgggcg tcccgctctt ccgcatccag gacctcgtcg
     4021 actaccacaa ggtcgccgtc ctcagctcga actacgcgct ctacggcgac atgtcgagcc
     4081 gcgtcatggc cgtgctccac gacctcgcgc ccgtcgtcga ggtctactcg atcgacgagg
     4141 ccttcctcct cctcgacctg cctcaagcgt ggcttcagga gttcgcccgc cgcgcgcgcg
     4201 agcgcgtcta ccgctggacg gggatccccg tctcggtcgg cgtcgccccg acgaagacgc
     4261 tcgccaaggt ggcgggcgag cgggcgaaga aaagtcccga cggcgtctgc gacctgaccg
     4321 accgtgagcg cgccgacgag gtgctcgctt ccctgcccgt cggcgacgtg tggggcgtcg
     4381 ggcgccagta cgcgaagctc ctgcgctcgc gggggatcga gacggcgaag cagctccgtg
     4441 actccgacga gcgctgggcc cgaaaggcga tgacggtggt cggcgcgcgc atcgtccacg
     4501 agctgcgcgg cacgagctgc ctcgcgctcg aggcggcccc gccggcgaag aagtcggtga
     4561 cctgctcgcg ctcgttcgga cagccggtag agacgccggg agagttgaag gaggccgtcg
     4621 cctactacac tcagagcgcc gccgagcgcc tccgcaggca ccgcctcgcc gccggcgccg
     4681 ttacggtctt cgtctcgacg aaccgcttct cgaagtcgga gccgcagtac tcgaactcgg
     4741 cgacggtcga agtggccttc cccaccgact cgacgcagga gctgctcgcg gtgacgctcc
     4801 gggcggcgga gaagatgttc cgcgagggct tcaggttcaa gaaggcggga gtgatcctgt
     4861 cggcgctcgt accctcgagc ccgatgacgg cgcgcatgta cggggacgcc ctctggcaga
     4921 agtcgcgccg gctgatgggt gccatcgacg agatcaaccg gaagttcggg aaggactcga
     4981 tcaggttcgc ggcgggcggc atgaggcggg tatggaagac gaaggcggag agggaatcgc
     5041 ccagatacac gacgtgctgg agcgatgtgc tcgcggttta aaccgtgggc gaggaagtga
     5101 tggtttcccg cacggcataa cagttggatt taaaggatag cgggggggag acttgaactc
     5161 ccgaccttgg ggttatgaat cccatgccaa gccccgcata tacgtcgtaa accggcgaaa
     5221 acctacaaga atggcgggcc gcgaacccta aatatccgta taagacgtat aagacgtata
     5281 tagattacgg caaaattacg gcaagcccca ccgcgtgcag gtgaagcacg ccggccatag
     5341 tcatcgcccg gcgcct
//
LOCUS       DBNGOH010000996         3582 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3631810, whole genome shotgun sequence.
ACCESSION   DBNGOH010000996 DBNGOH010000000
VERSION     DBNGOH010000996.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3582)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3582)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3582
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3631810"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..568
                     /locus_tag="AC159P_19430"
     CDS             <1..568
                     /locus_tag="AC159P_19430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697667.1"
                     /translation="KATGAKTLSRSSRRSLPVGGIRRGCRGGSWSRHTCPTATSSSAA
                     RHAPPRAGARSSKRRGTPAAASARPAGRPPAAPRFHAAHTARLSAVAGEGWLAGGDAA
                     VSHDPLASYGISAALGAGFYAAAAVADYLGGSREALRAYAQLIDRAFAQYLLLHHDRY
                     LSEQRWPDAPFWRRRHAPDFRGRENSPD"
     gene            complement(651..1517)
                     /locus_tag="AC159P_19435"
     CDS             complement(651..1517)
                     /locus_tag="AC159P_19435"
                     /inference="COORDINATES: protein motif:HMM:TIGR02646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="retron system putative HNH endonuclease"
                     /protein_id="HIR3697668.1"
                     /translation="MIRVNRKKVPLPPDLADDGKSTAARERLKTDAFYADKKNHQLPY
                     DKYAAYKKDSVIKALNDLFRGKCAYCESIYAATQPTDVEHYRPKGGVVVAGEFTRPGY
                     YWLAASWENLLPSCIDCNRERKQDFTDKTSGKSGKANHFPLTKEDYRAKSAAEIKREA
                     RARLLLDPCHDDPDKHLVFEDDGVVKPLKQKSGRASTRGENSIRIYGLNRYDLASLRA
                     ERARETLALIARIKRLEQRVELDPDDKFGFQAELLEEMALLKGYGQPDRRYAGMVRQL
                     VRRHYRKVDFDS"
     gene            complement(1520..>3582)
                     /locus_tag="AC159P_19440"
     CDS             complement(1520..>3582)
                     /locus_tag="AC159P_19440"
                     /inference="COORDINATES: protein motif:HMM:NF024700.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR3697669.1"
                     /translation="LFEAFNGKCAFCESPVAADSYMYLDRFRPRTDAINRDGSVSRDH
                     YWWLAYEWSNFYLICVACNKLKGNRFPTERARAEAEAVGAALLAEEPLLLDPCLDNPE
                     EHLLFDERGMVASDTKRGRTTIEVFNLNRADLVASRAAVFAETQAQWASVFDRRTSRS
                     KLPTEEFMAGLDDPTLTFLGLRRQFLQQWGREAVKKEPRLKKSLARCLSYRTTLSELV
                     KESAPRAGRSVLKKAFDNFDAFQARREKYSVARSAGIESYYLKTRLIEHIEIKNFKLI
                     GELSLSIPISTKQIGSWLVLLGENGTGKSTVLHAVALTLMGESHRRQLRLDASDFVRY
                     KCRSGHVKIRLTGSAEPIVLRFSKGSKRFDVTPKEPRVLLLGYGATRLLPKGKHKPAS
                     GGPATGQGAKAENLFNPFTPLNDAERWLGKLPSEDFELIARGLEDLLLLREDETLVRE
                     RSGPNARVFVKTSKSRVALSDLSDGYQSVIALTSDIMSVMRLRWETMAEAEGIVLIDE
                     IDSHLHPTWKMQIIAQLRKSFPRLQFVVSSHDPLTLRGLEAPEVVVMKRDEGGLPIAL
                     TDLPSPKSLRVDQLLTSEYFGLNSTVEPEVDRLFKEYYELLRKEKRTASEEQRLAELK
                     GRLDQHHQLGATRRERLMLEAADSFLALEERTPEASERRQLKERTKRKLAEMWKSVTP
                     AKSE"
BASE COUNT          518 a         1159 c         1210 g          695 t
ORIGIN      
        1 gaaggcgacg ggcgcgaaga ctctttcacg ctcgtcgagg cggtcgcttc cggttggtgg
       61 tattcggcgt ggctgccggg ggggaagttg gtcgcggcat acatgtccga cagcgacctc
      121 gtcgagcgcg gcgcggcacg caccgccgag ggctggcgcg cgctcctcga aacggcgcgg
      181 cacaccagcc gccgcgtcgg cgaggccggc tggtcgcccg cccgccgcgc cgcgcttcca
      241 cgcggcgcac acggcgcggc tctccgcggt cgcgggagag ggctggctcg cgggcggcga
      301 cgcggcggtc tcccacgacc cgctcgcgtc ctacggcatc tcggcggcgc tcggcgcggg
      361 gttctacgcg gcggcagccg tcgccgatta cctcggcggc agccgcgaag cgttacgcgc
      421 ttacgcgcag ctcatcgacc gcgcgttcgc gcagtacctc ctcctgcacc acgaccgtta
      481 cctctcggag cagcgttggc cggacgcgcc cttctggcga cgacggcacg cgcccgactt
      541 ccgcggaagg gagaactctc ccgactgaaa tccgtcgcct cactccttcg aataccgtgc
      601 ttgccttcat gtcgagtggc gggatgcagc cgtcaacgcc cgccgtcgcc tcaggagtcg
      661 aagtccactt tgcggtaatg gcggcgcacg agttgtctga ccatgcccgc gtacctccga
      721 tccggctggc cgtacccctt gaggagcgcc atctcttcca gcagctcagc ctggaaaccg
      781 aacttgtcgt cagggtcgag ttcgacccgc tgctccagcc gtttgatccg ggcgatgagg
      841 gccagcgtct ctctcgcacg ctcggcgcgc agcgaggcga ggtcgtagcg gttcaggccg
      901 tagattctga tcgagttctc gcccctcgtg ctggcgcgac cactcttctg cttcaagggc
      961 ttcaccacgc cgtcgtcctc gaacacaagg tgcttgtccg ggtcgtcgtg gcaggggtcg
     1021 aggaggaggc gggcgcgggc ctcgcgcttg atctcggcgg cactcttggc gcggtagtct
     1081 tccttcgtca gggggaagtg gttcgcttta ccggatttgc cgctcgtctt gtcggtgaag
     1141 tcctgcttgc gctcgcggtt gcagtcgatg caggacggca acaggttttc ccaactggcg
     1201 gccagccagt agtagccggg gcgcgtgaac tctcccgcga cgacgacgcc ccctttcggg
     1261 cggtagtgct cgacatcggt cggctgcgtc gcggcgtaga tgctctcgca gtaggcgcac
     1321 ttgccgcgga agaggtcgtt gagggccttg atgacgctgt cttttttgta ggcggcatac
     1381 ttgtcgtagg ggagctggtg attcttcttg tcggcgtaga aggcgtccgt cttgaggcgc
     1441 tccctggcgg cggtggattt accgtcgtcg gcgaggtcgg gcggcagcgg gactttcttg
     1501 cggttgactc ggatcatggc tattcactct tcgcgggtgt gacggacttc cacatctcgg
     1561 cgagcttgcg tttcgtccgt tctttcagtt ggcggcgctc ggacgcttcg ggggtgcgct
     1621 cttcgagggc gaggaaactg tccgcggctt cgagcatcag gcgctcgcgg cgcgtggcgc
     1681 cgagctggtg gtgttggtcg agccggccct tcaactcggc gaggcgctgc tcttcggacg
     1741 ccgtccgttt ctccttgcgc agcagctcgt agtattcctt gaacaaccga tccacctccg
     1801 gctcgaccgt gctgttgagg ccgaagtatt ccgaagtgag gagctgatcg acgcgcaggc
     1861 tcttcggcga cggcaggtcg gtcagcgcga tgggcaagcc cccctcgtcg cgcttcatga
     1921 cgaccacctc cggcgcctcc aacccgcgca gcgtcagcgg gtcgtggctt gagacgacga
     1981 attgcaggcg cgggaaagac ttccgcagtt gtgcgatgat ctgcatcttc cacgtcgggt
     2041 gcaggtgcga gtcgatctcg tcgatcaaca cgatgccttc cgcctccgcc atcgtctccc
     2101 agcgcaggcg catcacgctc atgatgtcgg acgtgagggc gatcaccgac tgatagccgt
     2161 cgctcaggtc cgagagcgcg acgcgggatt tggacgtttt gacgaaaacg cgggcgttcg
     2221 ggccgctccg ttccctgacc agcgtctcat cctcgcgcag cagcagcagg tcttcgagtc
     2281 cgcgcgcgat gagttcgaag tcttcactcg gcagcttgcc gagccagcgc tcggcgtcgt
     2341 tgagcggggt gaatgggttg aagagattct cggccttcgc cccctgcccg gtggccgggc
     2401 cgcccgaggc gggcttgtgc ttccccttcg ggagcagccg cgtcgcgcca tagccgagca
     2461 gcaggacgcg cggctccttc ggcgtcacgt cgaacctttt cgaccccttc gagaagcgga
     2521 ggacgatcgg ctcggcgctc cccgtgagac gtatcttgac gtgaccgctc cggcacttgt
     2581 agcggacgaa gtccgaggcg tcgagcctga gctgccggcg gtggctctcg cccatcaacg
     2641 tcagcgcgac ggcatggagc accgtgctct tgcccgtgcc gttctcgccg agcagcacca
     2701 gccacgagcc gatctgtttc gtcgagatgg ggatcgacag cgacagctcg ccgatcaact
     2761 tgaaattctt gatctcgatg tgttcgatga ggcgcgtctt caggtaatag ctctcgatgc
     2821 ctgccgagcg cgcgacggaa tacttctccc gacgcgcctg aaaggcgtcg aaattgtcga
     2881 aagccttttt gagcacagac ctgccggccc gcggggccga ctccttgacc agttccgaga
     2941 gggtcgtgcg gtaggagagg caccgcgcga gcgacttttt caggcgcggc tctttcttca
     3001 cggcttcgcg gccccattgc tggaggaact gcctgcgcag ccccaggaag gtgagcgtgg
     3061 ggtcgtcgag gcccgccatg aactcctcgg tcgggagctt cgaccgcgac gttcggcggt
     3121 cgaagaccga agcccactgc gcctgcgttt cggcgaagac cgcggcccgg ctcgcaacca
     3181 ggtcggcgcg gttgaggttg aatacttcga tcgtggtgcg cccgcgcttg gtgtcgctcg
     3241 ccaccatccc gcgctcgtcg aagagcaggt gctcctccgg gttgtcgaga cacgggtcga
     3301 gcagcagcgg ctcttctgcg agcagcgcgg cccccacggc ttccgcctcc gcgcgcgcgc
     3361 gctcggtggg gaagcggttc cccttgagct tgttgcacgc gacgcaaatg aggtagaagt
     3421 tcgaccactc gtacgcgagc caccagtaat gatcgcggct cacgctgccg tcgcggttga
     3481 tggcgtcggt gcgcgggcgg aagcggtcga ggtacatgta gctgtccgcg gcgacggggc
     3541 tttcgcagaa ggcgcacttg ccgttgaagg cttcgaagag cg
//
LOCUS       DBNGOH010000997         2367 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_882495, whole genome shotgun sequence.
ACCESSION   DBNGOH010000997 DBNGOH010000000
VERSION     DBNGOH010000997.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2367)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2367)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2367
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_882495"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(71..1315)
                     /locus_tag="AC159P_19445"
     CDS             complement(71..1315)
                     /locus_tag="AC159P_19445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009224642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HIR3697670.1"
                     /translation="MTDAANQPATDRPLSTADVSPPDAAASQPGQTRIGGLRIPTDSL
                     RAYTMVFALVVIWLFFHWTTDGIFLSPRNFSNLFKQTAVTGVLAVGMLMVIVAGQIDL
                     SVGSVVGLAGCCAAMTQEWGLVPSLGAAILIGTAIGAVQGGLVAYANIPAFIVTLGGM
                     LAWSGVTLDLSDGATIPVRLPFFRMLGVGLAAPAFGYAVAVAAIAAIVWLNLNRNRAR
                     RRHGLDVPGLGATASRIVTPSLLVVAFILMMNWRGDDLERQVGVPLPVLVLLVVAVIG
                     AFLTQNTTFGRYLYAIGGNPDAARLSGISLRKHILAAFCLLGALVGVASILHTARVGS
                     ASPDAGRLMELDAIAACVIGGTSLMGGRGTVFGALLGALIMASLDNGMSLRGTENSTQ
                     FIIKGAVLVAAVGFDMLGRRRG"
     gene            complement(1312..1956)
                     /locus_tag="AC159P_19450"
     CDS             complement(1312..1956)
                     /locus_tag="AC159P_19450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697671.1"
                     /translation="MSRKKFVTWLLILVALPWPIDYWRTRVAERKDRRDEELFRRHEC
                     GYDSPCAADFDRDGVGARFDILQPEAYRPYKLIVSDGGREVLRLPYHNIDYTLRTHLA
                     VHEQGGAARLLVYDGTERRPPVRAVYAWSGGRLVEATPDAFDIEILDALAAYDDTGTF
                     NERVFRSLISAVRFVAYYLLLLALAGIMLWGKLHLPDGPPPHTRDNHWLLRDDR"
     gene            complement(1996..>2367)
                     /locus_tag="AC159P_19455"
     CDS             complement(1996..>2367)
                     /locus_tag="AC159P_19455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014825021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR3697672.1"
                     /translation="RAMTDMRVKANSVFTVAGTLSGGNQQKVVLAKWLLTNPRVLFLD
                     EPTRGIDVGAKQEIYNHINRLAGRGLAIVLVSSELPEVLGLSDRVLVLHEGRVTGEFK
                     RAEATPEAVMSCATGHARRVA"
BASE COUNT          399 a          819 c          808 g          341 t
ORIGIN      
        1 gagcggcgcg acgagcagcg ccgtcaggag gagtgcgacg agggcgcggg tcttgttcat
       61 gtcttgagtg ctaccccctg cgccgcccga gcatgtcgaa gccgaccgcc gcgacgagca
      121 ccgcgccctt gatgatgaac tgcgtcgagt tctccgtgcc tcgcagcgac atgccgttgt
      181 cgaggctcgc catgatgagc gcgcccagga gcgcgccgaa gaccgtcccg cgcccgccca
      241 tcaagctcgt cccgccgatg acgcacgccg caatcgcgtc cagctccatc agacgccccg
      301 cgtccggcga ggcgctcccc acccgcgccg tgtgcaggat cgaggccacg cccaccagcg
      361 cgccgagcag acagaacgcg gcgaggatgt gtttgcgcag gctgatgccc gacagccgcg
      421 cggcgtccgg gttgccgccg atggcgtaga ggtagcgacc gaaggtcgta ttctgcgtga
      481 ggaacgcgcc gatcaccgcg accaccagca gcacgagcac cgggagcggc acgccgacct
      541 gccgttcgag gtcgtcgccc cgccagttca tcatcaggat gaaggcgacg acgagcagcg
      601 aaggggtcac gatgcgcgag gcggtcgcgc cgaggccggg cacgtccagc ccgtggcggc
      661 ggcgcgcgcg gttgcggttc aggttgagcc agacgatggc ggcgatggcc gccaccgcga
      721 ccgcgtagcc gaacgcgggc gcggccagac cgacgcccag catccggaag aaaggcaggc
      781 gcaccggtat cgtcgccccg tcgctgaggt cgagcgtcac cccgctccac gcgagcatcc
      841 cgccgagcgt gacgatgaac gcggggatgt tcgcgtaggc gaccagcccg ccctgcaccg
      901 cgccgatggc ggtgccgatc aagatggccg ccccgagcga aggcaccagc ccccactcct
      961 gcgtcatcgc cgcgcagcac ccggcgaggc ccaccacaga ccccaccgac agatcgatct
     1021 gccccgccac gatcaccatc aacatcccga ccgcgagcac gcccgtcacc gccgtctgct
     1081 tgaacaggtt cgagaagttg cgcgggctta aaaaaatccc gtccgtcgtc cagtgaaaga
     1141 agagccagat gacgacgagc gcgaagacca tcgtgtaggc gcgcagagag tcggtcggga
     1201 tacgcaggcc gccgatgcgc gtctgccccg gctgcgaagc ggcggcgtcc ggcggcgaca
     1261 cgtcggcggt cgagagcggg cggtcggtgg cgggctggtt tgcggcgtct gtcatcggtc
     1321 gtctctcaag agccaatggt tgtcgcgggt gtgcggcggc gggccgtccg gcagatggag
     1381 cttgccccac agcatgattc ccgcgagcgc gagcaggagt aaataataag cgacgaaccg
     1441 caccgcactg atgaggcttc ggaaaactct ctcgttgaag gtgccggtgt cgtcgtaggc
     1501 ggccagcgcg tcgaggattt cgatgtcgaa cgcgtccggc gtggcctcga cgagtctccc
     1561 gccgctccac gcgtatacag cccgcacggg cgggcgtcgc tcggtcccgt cgtacaccag
     1621 cagccgcgcc gcgccgccct gttcgtgaac cgcaaggtgc gtgcgcagcg tgtagtcgat
     1681 attgtggtag ggcaggcgca ggacttcgcg cccgccgtcg ctgacgatga gcttgtaggg
     1741 gcggtacgcc tcgggctgca agatatcgaa ccgcgcgccg acgccgtcgc ggtcgaagtc
     1801 cgccgcgcac ggcgagtcgt acccgcactc gtggcggcgg aacaactcct cgtcgcgcct
     1861 gtccttacgc tccgctacgc gcgtgcgcca gtaatcgatg ggccacggca gcgcaaccag
     1921 tatcagcagc cacgtgacga acttttttct gctcatagcc ggacgactcc cgttcgccgg
     1981 agcgctcctc acgaatcagg cgacccgccg cgcgtggccc gtggcgcacg acatgacggc
     2041 ctcgggcgtc gcctccgcgc gcttgaactc gccggtgacg cggccctcgt gcagcacgag
     2101 cactcggtcg gagaggccga gcacttccgg aagctccgag gagacgagga cgatggctag
     2161 ccctcggccc gcgagccggt tgatgtggtt gtagatttcc tgcttggcgc ccacgtcgat
     2221 gccgcgcgtc ggctcgtcca gaaagagcac gcgcgggttc gtcaggagcc acttggcgag
     2281 gacgaccttc tgctggttgc cgccgctcaa ggtgcccgcg acggtgaaga ccgagttggc
     2341 cttgacgcgc atgtccgtca tcgcgcg
//
LOCUS       DBNGOH010000998         4519 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4000464, whole genome shotgun sequence.
ACCESSION   DBNGOH010000998 DBNGOH010000000
VERSION     DBNGOH010000998.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4519)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4519)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4519
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4000464"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..633)
                     /locus_tag="AC159P_19460"
     CDS             complement(<1..633)
                     /locus_tag="AC159P_19460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697673.1"
                     /translation="MRADVSIRTALPPSASSVSTLKLAVVLIAGVVSVGTYGAGFASR
                     PRPSVEKQPVAEQGGEILGDEVASLDAMVRQIEGEDFSRPGDREKVLKGALNFFRPFP
                     GGNGRSCATCHDPRDGFSLSPATVEARWRRLQSARRRNPNADDPLFRSIDADDSQQDF
                     TLLRTRALVKVRVPLPDRVRLTDDPAATHVTLSRAVPPLNMLKHTAPYQQD"
     gene            complement(796..1287)
                     /locus_tag="AC159P_19465"
     CDS             complement(796..1287)
                     /locus_tag="AC159P_19465"
                     /inference="COORDINATES: protein motif:HMM:NF023520.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3574 domain-containing protein"
                     /protein_id="HIR3697674.1"
                     /translation="MHGFVKKRFLALALLTTSLLAPATYARQQTAPTATQQTSARSRA
                     ARLKQVASDPFIRTELFFGTDREEGPDVTEAEFREFLDRVITPSFPDGLTLLTGDGQF
                     CCDANNEIIKEKSFILVLLYPLRQRRESSEKIERIREEYKTAFRQQSVLRVDAPLPVW
                     VSF"
     gene            complement(1331..2299)
                     /locus_tag="AC159P_19470"
     CDS             complement(1331..2299)
                     /locus_tag="AC159P_19470"
                     /inference="COORDINATES: protein motif:HMM:NF024627.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HIR3697675.1"
                     /translation="MASAVLLLTLACVADARAQAGRSATRVWVSATAGSDANPCTRAE
                     PCRDLSTAVNAVVEDGEVVILDSGVYDAVEITKSVQVVAPEGVHAVIAPAAGIPVPES
                     ATGATAAVLINAPDATVILRNLTVNREGAIHIGIRVSSVRTLQIENCVVNAFNGPGLE
                     YGGIFFEASGRLSVRDTTLRSNSRGIVVTAPSGGTAHASIDGCRLDGNTFGVVAFGGA
                     RTTVSNTVAANGSATGFAVSAAPGTTSHFSCDHCTASNLTMGFVAGEGAGAVMRVSNS
                     VATNNSYGFYKSGLYSTFKSLAGTNMVEGNQTNRFGQIITFSPDAP"
     gene            complement(2436..>4519)
                     /locus_tag="AC159P_19475"
     CDS             complement(2436..>4519)
                     /locus_tag="AC159P_19475"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HIR3697676.1"
                     /translation="SEAAWARLGAEERRPFEAYVAGVNAYLAELKSGQLPLEFRILGF
                     EPEPWRPEDVLVWGKVVAWGIGGNWDKELLRARIAAKLGPARAAQLMPDYTADGPTIL
                     PPAHRPAEAARAVSDTNPQPGAPDLDPTLVDGLLELSHQVKALTGLGADGFGSNNWVL
                     SGSRTTTGRPLLAGDPHLSAQTPAIWYLARVSGGALDVTGATIPGGPGVYIGHNSRIA
                     WAPTTINVDSQDLYVEHVNERGEAEFMGGWEPLTVVPETIKVKGGPDVNLKVRSTRHG
                     PLISDVVNPAGQPLALRWTANDPVDAGALATLGYNRARNFKQFTEASRAHRPADQNYV
                     YADRKGHIGYIAAATIPVRPAGDDGRLPVPGWTGSHEWSGYVPFDELPRAYDPPQGFI
                     ASANNRVAGDDYPHLIGTNFAAPYRAARIVELIEGQSRHSADDVSRMQADVLALHARE
                     VLPFMLAADPLDERGRLAAGLLRGWDLRVTGESAPAAVFESWYLKLGERLFADELSDP
                     VSGDALWRAYSEHIYMVGMALEAALEENRTWCDDVRTPEAETCADTLASSLSEGLARM
                     AAAQGTDEPAAWRWDRAHRALFPHAPFDADPQLRPVFSRSIPNGGDKFTVNVASIFLW
                     GDYDQLHAAQYRQVVDFGEPSNSRFVVAPGQSGDPASAHYDDLLELWRSVEYLPMPFI
                     RKAPGGAAPQP"
BASE COUNT          667 a         1499 c         1608 g          745 t
ORIGIN      
        1 gtcctgctgg tagggggcgg tgtgcttgag catgttgagc gggggcacgg cgcgcgagag
       61 cgtcacgtgg gtggccgccg ggtcgtcggt cagccgcacg cggtccggga gcggcacacg
      121 caccttgacg agcgcgcggg tgcgcagcag cgtgaagtcc tgctggctgt cgtctgcgtc
      181 aatcgaacgg aagagcgggt cgtcggcgtt cgggttgcgt cgccgcgcgc tctgtagccg
      241 ccgccagcgg gcttcaacgg tcgcgggcga gagactgaat ccgtcgcgcg ggtcatgaca
      301 ggtggcgcac gagcgcccgt tgccgccggg gaaaggccgg aagaagttca gcgccccctt
      361 gagcaccttc tctctgtcgc cggggcggct gaaatcctcg ccttcgatct gccgcaccat
      421 ggcgtcgagg ctggcgacct catcgccgag gatttctccc ccctgctccg cgaccggctg
      481 tttctctacg gacggccgcg ggcggctggc aaaaccggcg ccataagtgc cgacgctcac
      541 cacaccggct atcagcacca cggccagctt aagtgtgctg acggaagacg cggacggggg
      601 caaggccgtc ctgatcgata catcagctct catcatttga tgtctccacc tcttgccacg
      661 ccagctaagt ctgtgactac cacccgctga cattcatccc gagcgagtcg aaccccgcca
      721 caggccgcat gggcatcgcc ggtatgacaa tggccatgcg ggtctctccc ggttgtactc
      781 gcacaaaacg cgcgctcaga acgagaccca cacgggcagg ggcgcgtcca cacgcaggac
      841 ggactgctga cggaacgccg tcttgtactc ctcgcgtatc ctctcgatct tctcgctgct
      901 ctcccgccgc tggcgcagcg ggtagaggag aacgaggatg aagctcttct ccttgatgat
      961 ttcgttgttg gcgtcgcagc agaactggcc gtccccggtc aagagggtca gcccgtccgg
     1021 gaatgagggc gtgatgaccc tgtcgaggaa ctcgcggaac tcggcctcgg tgacatcggg
     1081 gccctcctcc cggtcggtgc cgaagaacag ctcggtgcgg atgaacgggt ctgaggcgac
     1141 ctgcttgagc ctggcggcac ggctccgtgc cgacgtctgc tgtgtggccg tgggcgccgt
     1201 ctgctgccgc gcgtatgtcg ccggcgcgag cagcgaggtc gtgagcaagg ccagtgcgag
     1261 aaatctcttc ttcacgaatc cgtgcatcgt ctgggaactc ctttctctgg cggaaatggc
     1321 ctcgcgacgt ttacggcgcg tccgggctga aagtgatgat ctgtccgaac ctgttcgtct
     1381 ggttcccttc gaccatgttc gtgcccgcca gtgacttgaa cgtgctgtac aagccgctct
     1441 tataaaagcc gtaggagttg ttcgtcgcca ccgagttgga aacgcgcatc acggcgcccg
     1501 cgccctcccc ggcgacgaag cccatggtga ggttgctcgc ggtgcaatgg tcgcagctaa
     1561 agtggctggt cgtacccggg gcggcggaga cggcaaagcc cgtggcggaa ccgtttgccg
     1621 ccaccgtgtt gctcaccgtc gtacgcgcgc cgccgaaggc aacgacgccg aaagtgttgc
     1681 cgtcgagccg gcacccgtcg attgaggcgt gggccgtgcc gcctgaaggc gcggtgacga
     1741 cgatgccgcg gctgttgctg cgcagcgtgg tgtcacgcac cgacagccgc ccgctggcct
     1801 cgaaaaagat gccgccgtat tccagcccgg gaccgttgaa ggcattgacg acgcagttct
     1861 cgatttgcaa cgtgcggacg gacgaaacgc ggatgccgat atggatagct ccctccctgt
     1921 tgacggtgag gttccgcagg atgacggtcg cgtccggcgc gttaatgagc accgcggccg
     1981 tcgcgccggt ggcgctctcc ggcacgggga tcccggccgc cggggcgatc acggcgtgga
     2041 cgccctccgg cgcgacgacc tgcacggact tcgtgatctc gactgcgtcg tagacgccgc
     2101 tgtcgagtat gacgacctcg ccgtcctcga cgaccgcgtt gacggcggtc gagaggtcac
     2161 ggcagggctc tgcgcgcgtg caggggttcg cgtcgctgcc cgccgtcgcc gagacccaca
     2221 cgcgcgtcgc ggagcggccg gcctgcgcgc gcgcgtccgc aacgcaggcg agggtcagga
     2281 gaaggactgc ggacgccacg gcgcacagcc gcgccgcagg tgacgtgtga aagtattgcc
     2341 tcacgataac tggtctcctt tgagttcaaa gtaatcggca aaaaaccgag tcgggtgtga
     2401 agggacggcg cgcctcgcgg cgaacggccc gggcgtcaag gctgcggcgc cgcgccgccg
     2461 ggcgccttgc ggatgaaggg catcggcagg tactcgacgc tgcgccagag ttcgagcagg
     2521 tcgtcgtagt gcgcgctcgc ggggtcgccg gactggcccg gcgcgacgac gaagcgcgag
     2581 ttcgacggtt cgccgaagtc cactacctgc cggtactggg ccgcgtggag ctggtcgtaa
     2641 tctccccaga ggaagatcga cgcgacgttg accgtgaact tgtcgccgcc gttagggatg
     2701 ctgcggctga agacgggccg gagctgcggg tcggcgtcga acggggcgtg cgggaagagc
     2761 gcgcggtgtg cgcggtccca acgccacgcc gccggctcgt ctgtgccctg cgccgcggcc
     2821 atccgcgcca gcccttcgga gagtgacgag gcgagcgtgt ccgcacaggt ctctgcctcc
     2881 ggcgtccgta cgtcgtcgca ccaggtccgg ttctcttcga gcgcggcttc gagggccatg
     2941 ccgaccatgt agatgtgctc cgagtaggcg cgccacagcg cgtcgcccga gacggggtca
     3001 gagagttcgt cggcgaagag tcgctcgccg agcttgagat accacgactc gaagaccgcg
     3061 gccggcgcgc tctcgcccgt gacgcgcagg tcccagccgc ggagcagccc ggcggccagc
     3121 cgcccgcgct cgtccaaggg gtcggccgcc agcatgaagg gcagcacctc gcgggcgtgc
     3181 agggccagca cgtccgcctg catccgtgac acgtcgtccg ccgagtggcg actctggccc
     3241 tcgatcagct cgacgatgcg cgcggcgcgg tagggggcgg cgaagttggt cccgataagg
     3301 tgcgggtagt cgtcgcccgc gacccggttg ttggccgacg cgatgaagcc ctgcggcggg
     3361 tcgtaggcgc gcggcagctc gtcgaacggc acgtagcccg accactcgtg actccccgtc
     3421 cagccgggca cagggagtcg gccgtcgtcg cccgcggggc ggacggggat cgtcgccgcc
     3481 gcgatgtagc cgatgtggcc tttgcggtcg gcgtagacgt agttttggtc ggcgggccgg
     3541 tgcgcgcgcg aggcctcggt gaactgcttg aagtttctcg cccggttgta cccgagcgtc
     3601 gccagggcgc ccgcgtccac ggggtcgttc gccgtccagc gcagggcgag cggctggccc
     3661 gcggggttga cgacgtccga gatgagcggg ccgtggcgcg tgctgcggac cttgaggttg
     3721 acgtccggcc cgcccttgac cttgatcgtc tcgggcacga ccgtgagcgg ctcccacccg
     3781 cccatgaact cggcctcgcc gcgctcgttg acgtgctcga cgtagaggtc ctgcgagtcg
     3841 acgttgatgg tggtgggcgc ccaggcgatc cggctgttgt ggccgatgta gacgccgggg
     3901 ccgccgggga tggtcgcgcc ggtcacgtcg agcgcgccgc cgctcacgcg cgccaaatac
     3961 cagatggccg gcgtctgcgc gctgaggtgc gggtcgcccg cgagcagggg ccgccccgtc
     4021 gtcgtgcgcg agccagagag cacccagttg ttgctcccga agccgtccgc gccgagcccg
     4081 gtcagcgcct taacctgatg gctgagctcc agcaggccgt ccacgagtgt cgggtcgagg
     4141 tcgggcgcgc ccggctgagg gtttgtgtcc gagacggcac gggccgcttc cgccgggcgg
     4201 tgcgcgggcg gcagaatcgt cggcccgtcc gcggtgtagt cgggcatcag ttgtgccgcc
     4261 cgcgcggggc cgagcttcgc cgcaatgcgc gcgcgcaaca gctccttgtc ccagttgccg
     4321 ccgatgcccc aggccaccac cttgccccag accagcacgt cctccggccg ccatggctcg
     4381 ggctcgaagc cgagtatccg gaactcgagc gggagctggc cgcttttgag ttcggcgagg
     4441 taggcgttga cgcccgcgac gtatgcctcg aatggcctgc gctcttccgc gccgaggcgc
     4501 gcccacgccg cttctgacg
//
LOCUS       DBNGOH010000999         3497 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1801687, whole genome shotgun sequence.
ACCESSION   DBNGOH010000999 DBNGOH010000000
VERSION     DBNGOH010000999.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3497)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3497)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3497
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1801687"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(105..1298)
                     /locus_tag="AC159P_19480"
     CDS             complement(105..1298)
                     /locus_tag="AC159P_19480"
                     /inference="COORDINATES: protein motif:HMM:NF042732.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6683 family protein"
                     /protein_id="HIR3697677.1"
                     /translation="MAVVLAFVSSFGAAAAAAQSSDRIRETWKGPDAALLTSLLKNRD
                     TNQPPNAAARPRMEVVKFTPAGDSGVAKSLGDAFGRTDEERAAVAEAFRRIKQGYEAE
                     VAREGKSNNLAAAMAFFIAANVAAYHQAELPADAATDKLFQSLQETMAGVPAFARLSN
                     PEKQQLHDWLVCMAGFVLTGYTEAKQSGDRESLKSFGELADYSMRLVLGVEVGKLSFA
                     GDELAIKGEGPAARPAGATGGGVVGVWSKSSSSPVGMAGTLDATQKQMVYPGYYKGEY
                     RFNADGTYAFKAERRGGSTGQGFWTTEESGTYAVNGDSLVISPRASKMTLRSLEGAVQ
                     KTQNNPLEKVTYRWRTHYFEGLDETQLVLQPPSQTTRDGGFSGNSLFPNSYLYSPGSK
                     LEFGF"
     gene            complement(1428..>3497)
                     /locus_tag="AC159P_19485"
     CDS             complement(1428..>3497)
                     /locus_tag="AC159P_19485"
                     /inference="COORDINATES: protein motif:HMM:NF018884.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HIR3697678.1"
                     /translation="GLTPIILLVVLGMLAAMGSSSQGMVRVVSVQVSANERQTLEAAR
                     MIAGGLVKPSSPDPARAGEATQAWLDERAALLRASLPGARVDVWRAPTPGGVGQLGHD
                     AEALASSGAEDERTRGVGVVADTPGAPLPAWLRGRDEWGGVAYLAPPESSESAFGTPS
                     LRALVRRADGGRAAAVLVTVPVSRALVEHYRETTGVNVHPFFIGAEQPEAEPGGGNIH
                     LEGNVDTDDRGNLKSDSRITFTGDRKKLVVDFRSDQFGETLPNAYPVFLDATNWANGA
                     KSTRLAFLLDWSWEGIWKQFWSDSVGQMWWRVLYGAAITFLVLELLALFSAAWMTRAV
                     TGTVHKLYRATEFIKRGDFSHRIRTRSRDQLGELAVAFNDMSADIEALLADRVERERL
                     EREIEIAHDVQAQFFPRSVPLLSTAEIIGECRAARGVSGDYYDYVEVAPGLIAFALGD
                     VSGKGISASLVMSNLQASLRAQAAIISERLRIARLPAAAASSTAAGGGVVVAPKSADG
                     GDAASTTTAGDVMPCGVAGVDTQCAVSNMAESINAQLCSSTEGNRFATLFLALYDDRT
                     RALRYTNAGHNAPALVRASGAVERLTRGGTMIGAFDWARFEEAQTTLHDGDLLLVFSD
                     GLSEAQNHAGEEYGEKRLVEFAAARRDHTVEDLRRAVFEEVNQWSGGAERDDDQTLVI
                     LKTVKRA"
BASE COUNT          534 a         1241 c         1138 g          584 t
ORIGIN      
        1 acacggaggg cactgagaaa accacttctt ctctgtgccc tctgtgagtc ctccgtgccc
       61 tccgtgttaa aacgaacccg cgccggactc aaccgcgaac cgcactagaa tccgaactca
      121 agcttgctgc ccggactgta gaggtatgag ttcgggaaca ggctgttccc cgagaaacca
      181 ccgtcgcgcg tcgtctgact cggcggttgc aggacgagct gcgtctcgtc gagtccctcg
      241 aagtagtgcg tgcgccacct gtaagtcact ttttcgagcg ggttgttctg cgttttctgg
      301 acggcgcctt cgagacttcg caaagtcatt ttactggccc gcggagagat cacgagcgaa
      361 tcgccgttga ccgcatacgt cccgctctcc tcggtcgtcc aaaacccctg accggtagaa
      421 ccgccccggc gctcggcctt gaaggcgtaa gtcccgtcgg cgttgaagcg atactcacct
      481 ttgtaatagc cgggatagac catctgtttc tgcgtcgcgt cgagcgtgcc ggccatcccc
      541 accgggctgc tggagctttt actccacacg ccgacgacgc cgccgccggt cgcgcccgcg
      601 ggccgggctg cggggccttc gcccttgatc gccagctcgt caccggcgaa gctcagcttg
      661 ccgacctcga cccccagcac cagccgcatc gagtagtcgg ccagctcgcc gaaactcttc
      721 aggctttccc ggtcgccgct ctgcttcgcc tccgtgtagc ccgtcaacac gaagccggcc
      781 atgcacacca gccagtcgtg caactgctgc ttctcggggt tcgacaggcg cgcgaacgcg
      841 gggacgcccg ccatcgtctc ctgtaaggat tggaacagct tgtcggtggc cgcgtccgcg
      901 ggcagctcgg cctgatgata agccgccacg ttcgcggcga tgaagaaagc catcgcggcg
      961 gcaaggttgt tcgacttccc ctccctggcg acctccgcct cgtaaccctg tttgatccgt
     1021 ctgaaggctt cggcgacggc ggctctctct tcgtcggtgc gcccgaacgc gtcgcccagg
     1081 gatttcgcca cgcccgaatc gccggcgggg gtgaacttga cgacctccat gcgcggcctc
     1141 gccgcggcgt tcgggggctg gttcgtgtcc ctgttcttca gcaggctcgt cagcaaggcc
     1201 gcgtccgggc ctttccacgt ttcacggatg cggtcggagg attgggcggc agcagcagcc
     1261 gcgccgaagg acgacacgaa ggcgagaacc accgccatga ttcgtaagct tcttgagtcc
     1321 agcttcaacc tcacgagtcc tccctcgaat cgttaagccc ccgccctgtc ggcgacacgt
     1381 aagaccgcgc agccgctctt ctcttaccgc ggttcacgat ttacgactca cgcacgcttc
     1441 acggttttca agatcacgag cgtctggtcg tcgtcgcgct cggcgccgcc cgaccactgg
     1501 ttgacctctt cgaagacggc gcggcgcaag tcttcgaccg tgtggtcgcg gcgcgcggcg
     1561 gcgaactcga cgagacgctt ctcgccgtac tcttcgcccg cgtggttctg cgcctcgctg
     1621 aggccgtcgg agaagacgag caggaggtcg ccgtcgtgga gcgtggtctg cgcctcttcg
     1681 aaccgtgccc agtcgaaggc gccgatcatc gtgccgccgc gggtgagccg ttcgacggcg
     1741 cccgaggcgc ggacgagcgc gggcgcgttg tggccggcgt tggtgtagcg gagcgcacgc
     1801 gtgcggtcgt cgtagagcgc gaggaagagc gtggcgaagc ggttgccctc ggtgctgctg
     1861 cacagttgcg cgttgatgct ctcggccatg ttcgagacgg cgcactgcgt gtccacgccc
     1921 gccacgccgc acggcatcac gtcgcccgcc gttgtcgtcg aagccgcgtc gccgccgtcc
     1981 gcgctcttcg gcgcgaccac gacgccgccg cccgccgcgg tggacgaggc cgccgccgcg
     2041 ggcagccgcg cgatccgcag ccgctcggag atgatggccg cctgcgcgcg cagagaagcc
     2101 tgcaagttcg acatcacgag cgaggccgag atgcccttgc ccgacacgtc gccgagcgcg
     2161 aaagcgatga ggccgggcgc aacctcgacg tagtcgtagt agtcgcccga gacgccgcgc
     2221 gcggcgcggc actcgccgat gatctcggcg gtcgagagta ggggcacgct gcgcgggaag
     2281 aactgcgcct gcacgtcgtg cgcgatctcg atctcgcgct ccagccgctc gcgctcgacg
     2341 cggtcggcca gcagcgcctc gatgtcggcg gacatgtcgt tgaaggcgac ggccagctcg
     2401 cccaactggt cgcgcgagcg cgtgcggatg cggtgcgaga agtcgccgcg cttgatgaac
     2461 tcggtcgcgc ggtagagctt gtgcacggtg cccgtcacgg cgcgcgtcat ccacgcggct
     2521 gaaaagagcg cgagcagttc gaggacgagg aacgtgatgg ccgcgccgta gagcacccgc
     2581 caccacatct gcccgacact gtcgctccag aactgcttcc agatgccttc ccaagaccag
     2641 tcgagcagga aggccaggcg cgttgacttg gcgccgttcg cccagttggt cgcgtccaga
     2701 aagacgggat acgcgttggg cagcgtctcg ccgaactggt cgctccggaa atcgacgacc
     2761 agtttcttcc tatcgccggt gaaagtgatc cggctatcgc tcttcaggtt gccgcggtcg
     2821 tcggtatcga cgttgccttc gagatgaatg ttgccgccgc ccggctccgc ttcgggctgc
     2881 tcggcgccga tgaagaaggg gtgaacgttg acgcccgtcg tctcgcggta gtgttcgacg
     2941 agggcgcggc tgacggggac ggtgacgagc accgccgccg cgcggcctcc gtcggcccgg
     3001 cgcaccagcg cgcgcagcga aggcgtgccg aaggcgctct cggaactttc gggcggcgcg
     3061 agataagcga cgccgcccca ctcgtcgcgc ccgcgcagcc acgcgggcag cggcgccccc
     3121 ggcgtgtcgg cgacgacgcc gacgccgcgc gtccgctcgt cctcggcccc gctcgacgcc
     3181 agcgcctccg cgtcgtggcc gagctgcccg accccgcccg gcgtcggcgc gcgccacacg
     3241 tccacgcgcg cccccggcaa actcgcgcgg agcagcgccg cccgctcgtc gagccacgcc
     3301 tgcgtcgctt cgcccgcgcg cgcggggtcg ggcgacgaag gcttcaccaa cccgcccgcg
     3361 atcatccgcg cggcctccaa cgtctgccgc tcgttcgcgc tcacctgcac ggagacgaca
     3421 cgcaccatgc cctgcgaaga gctgcccatc gcggccagca tgccgagcac cacgagcagg
     3481 atgatcggcg tcagccc
//
LOCUS       DBNGOH010001000         2708 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3815904, whole genome shotgun sequence.
ACCESSION   DBNGOH010001000 DBNGOH010000000
VERSION     DBNGOH010001000.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2708)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2708)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2708
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3815904"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            62..>2708
                     /locus_tag="AC159P_19490"
     CDS             62..>2708
                     /locus_tag="AC159P_19490"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR3697679.1"
                     /translation="MNAERWRQIDEVFDAALELEPEARAGFLSDRCGGNAELRREVEA
                     LLAAHDKAGGFIETSAMKVAARAVAGDTFYAEVERQIGPYRVLSLLGAGGMGEVYLAE
                     DTRLGRRVALKLLPPPFVSDPDRLRRFEREARAASALNHPNILTLYDIGRVDGTHYIV
                     TEYVEGGTLRARLSAGRITAPEAVRVAAQVAEALSAAHAAGITHRDIKPENIAIRPDG
                     YAKVLDFGLAKLAELPGGETDSLSPSLRTPMGLRMGTVKYMSPEQVLGHAVDGRTDLW
                     SLGVVLYETLTGQAPFAGANKAATFDLILNHTPAPVTDFDEELPPQLDAIVSRALEKD
                     PELRYQTASDMRAELRRLQRELEPFSSRWGRAVTRFRRAPARARGWRLKAALAACLLA
                     LSVVGAWLLFSPRPAPPEEARVTGPNWARAKSTQLTSGAGTEYSPSLAPDGRSLIYAS
                     RAAGNWDIYWQRVGGTNAVNLTKDSAADDTQPAYSPDGDLIAFRSERAPKGIYVMEAT
                     SENVRRLSDFGYDPSWSPDGKALVISSAQFLKPTTRSLLPGALWVIDVATGARRRLTE
                     GDAVQPSWSPNGQRVAYWGVQPGGGQRDIWTMDAGGGEAVPVTNDEALDWNPVWSPDG
                     KYLYFASNRGGSMNFWRVSIDERTGRTAGEPEAVTTPSAYSQHLSFSRDGKRMAYVQK
                     SETRNLQRVAFDPVGGKTAGAPESVTQGAMHVSAPDVSPDGEWLAYSSQGERQEDIFL
                     IRKDGTARRQLTNDHFADRSPRWSPDGSRIAFYSDRSGRYEVWLISPDGTGLRQLTFT
                     SGQSAVYPVWSADGRHIVFKQRDLMPFVVEADQPWASQTPRQLPPLPKEGGGHFWTWS
                     WSADGRRLAGWWVDQEAAKTYVYAYD"
BASE COUNT          447 a          925 c          944 g          392 t
ORIGIN      
        1 caaggaagaa ctgcttttat cttgtcacgg tttacggttc acggttcacg gctctttatt
       61 tatgaacgcc gagcgctgga ggcagataga cgaagtcttc gacgcggccc tggaactcga
      121 accggaggcc cgcgcgggct tcctgtccga ccgttgcggc ggcaacgcgg agttgcggcg
      181 cgaggtcgag gcgctgctcg cggcgcacga caaggccgga gggttcatcg agacctcggc
      241 gatgaaggtg gccgcgcgcg cggtggccgg cgacacgttc tacgccgaag tcgaacgaca
      301 gatcggcccg taccgtgtcc tctcgctgct cggcgcggga ggcatgggcg aggtctatct
      361 ggccgaggac acgcgactcg gtcgccgcgt cgccctgaag ctcctcccgc cgccgttcgt
      421 ctccgacccc gaccgcctgc gtcgcttcga gcgcgaggcg cgcgccgcct cggcgctcaa
      481 ccacccgaac atcctcaccc tctacgacat cgggcgcgtg gacggcacgc actatatcgt
      541 cacggagtac gtcgagggcg gaactttgcg cgcgcgtctg agcgccggtc gcatcacggc
      601 gcccgaagcc gtccgcgtcg cggcgcaggt ggccgaggcc ctctccgcgg cgcacgccgc
      661 aggcatcacc caccgcgaca tcaagcccga gaacatcgcc atcaggcccg acggttacgc
      721 gaaggtgctc gacttcggtc tggcaaagct ggcggaactg ccggggggcg agacggattc
      781 tctctcgccg tcgctgcgga cgccgatggg cctccgcatg ggaacggtca agtacatgtc
      841 gccggagcag gtactggggc acgcggtgga cgggcgcaca gacctctgga gtctgggcgt
      901 cgtgctctac gagacgttga cgggacaggc gccgttcgcg ggcgcgaaca aggcggccac
      961 cttcgacctg atcctcaacc acacgcccgc gcccgtgacg gacttcgacg aagagctgcc
     1021 cccgcagctc gacgccatcg tcagccgcgc gctggagaaa gacccggagc tgcgctacca
     1081 gacggcctcc gacatgcgcg ccgagctgag gcgtttgcag cgcgagctgg agccgttctc
     1141 gtcgaggtgg gggcgcgccg tgacgcgctt ccggcgcgcc cccgcgcgcg cgcgcggctg
     1201 gcgcctcaag gcggcgctcg ccgcgtgcct actggccctg tcggtcgtcg gcgcatggct
     1261 cctattctcg ccccgccccg cgccgccgga ggaggcccgt gtgaccggcc cgaactgggc
     1321 gcgcgccaaa tcgacgcagc tcacgagcgg ggccggcacg gaatactccc cgagcctcgc
     1381 cccggacggc aggtcgctca tctacgccag ccgcgccgcg ggtaactggg acatctactg
     1441 gcagagggtc ggcgggacga acgcggtcaa cctcacgaag gactccgcgg cggacgacac
     1501 acagccggcc tattcgccgg acggcgacct gatcgccttc cgctcggagc gcgcgcccaa
     1561 aggcatctac gtcatggagg cgacgagcga aaacgtcagg cgcctctcgg acttcggtta
     1621 cgacccttcg tggtcgcccg acggcaaagc gttggtcatc agctcggccc agttcctcaa
     1681 gccgacgacg cgcagcctgc tccccggcgc gctgtgggtg atcgacgtgg cgaccggggc
     1741 caggcgccgg ctgaccgagg gcgacgccgt ccagccgagc tggtcgccga acggtcaacg
     1801 ggtcgcgtac tggggagtcc agccgggcgg cgggcagcgg gacatttgga cgatggacgc
     1861 agggggcggc gaggcggtgc ccgtcacgaa cgacgaggcg ctcgactgga acccggtctg
     1921 gtcgccggac gggaagtacc tctacttcgc cagcaaccgc ggcggcagca tgaacttctg
     1981 gcgcgtctcg atagacgagc ggacggggcg gacggcgggc gagccggagg ccgtcacgac
     2041 cccctcggct tacagccaac acctcagctt ctcgcgcgac ggcaagcgga tggcttacgt
     2101 gcagaagtcc gaaacacgaa acctgcaaag ggtcgcattc gaccccgtcg gcgggaagac
     2161 ggcgggcgcg cccgagtccg tcacacaggg cgccatgcat gtcagcgcgc cggacgtgtc
     2221 gcccgacggc gagtggctgg cctacagctc gcaaggggag aggcaggagg acatcttcct
     2281 catcaggaaa gacgggacgg cccggcggca actgaccaac gaccatttcg ccgaccgctc
     2341 cccgcgctgg tcgcccgacg gcagccgcat cgccttctat tcggatcgga gcggtcgcta
     2401 cgaagtctgg ctaatcagtc cggacggcac ggggctgcgg cagttgacgt tcacttcggg
     2461 gcagtcggct gtctacccgg tctggtcggc ggacggcaga cacatcgtct tcaaacagcg
     2521 cgatctgatg cccttcgtcg tcgaggcgga tcagccgtgg gcatcgcaga ccccccgaca
     2581 gctaccgccc cttccgaagg aggggggcgg gcatttctgg acgtggtcgt ggtcggcgga
     2641 cgggcgacgg ctggccggct ggtgggtgga tcaggaagcc gcgaagactt acgtctacgc
     2701 ctacgatt
//
LOCUS       DBNGOH010001001         4533 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4549258, whole genome shotgun sequence.
ACCESSION   DBNGOH010001001 DBNGOH010000000
VERSION     DBNGOH010001001.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4533)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4533)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4533
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4549258"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(117..467)
                     /locus_tag="AC159P_19495"
     CDS             complement(117..467)
                     /locus_tag="AC159P_19495"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016701098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HIR3697680.1"
                     /translation="MRKGISIEEIKLGEGASAGRGDTVTIRYDGFLRRGDAFQRGAVE
                     SFKLGGRQVIAGLEYGVEGMRAGGRRRIRVAPHLGYGDRGVPGVVPANALLTFDVELL
                     EVEEGGRESSSGAC"
     gene            complement(535..1107)
                     /locus_tag="AC159P_19500"
     CDS             complement(535..1107)
                     /locus_tag="AC159P_19500"
                     /inference="COORDINATES: protein motif:HMM:NF025675.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YcxB family protein"
                     /protein_id="HIR3697681.1"
                     /translation="MEVEYEITTEDLFAYQWRACYKSPITRRARRKAYIYLLLLLLVL
                     SLVGAFGSGGFSLSLANFAWVAVAFPVLACMIRYSDRRQTRRAIRELLKDEKPDKGQL
                     GVHRIALTETGLVERTAVGESRVTWAGVDRVEQNEEYVFIYTAPSAAHIIPKRAFGSP
                     REAESFYRLAGVSKEAASVATTSTLPGGPS"
     gene            complement(1143..1595)
                     /locus_tag="AC159P_19505"
     CDS             complement(1143..1595)
                     /locus_tag="AC159P_19505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697682.1"
                     /translation="MDWYRRARLLFDVPKPAHFGNYRHCCECAEHDETLSAHDVESIG
                     LAQLGQPAWDPLCFSSAEGLVYYMPALVRITLETIGSPGEVYLDQFLFHLIQDGERNR
                     LVGACSAEQRAFVAGFLEFLIDNHAPRIEEGAYSTDDILRAHEIWSAG"
     gene            complement(1825..3297)
                     /locus_tag="AC159P_19510"
     CDS             complement(1825..3297)
                     /locus_tag="AC159P_19510"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3697683.1"
                     /translation="MSTARTSTSIKGAMLLLVIGLFPHAAFGQEARGGRKEAERFFAR
                     GLDFYRGGKFEEAVASYGQAIKYERPDAGQLYLMSLAHLQLGSYLEASRLLEKSVQLH
                     PKNTASLAALGALYTYLGRYAEAEKVLKQAHGVGGPSAEVLNNLGIAYQRGGRSRDAV
                     GALNQAVKLAPSDAVIHHNLSDAYLKLGQTDEAYEAKLRADRLAPRNTNNAGVILLLS
                     GPLKLELKAPNDPPGVSGGEPVRPGGAAEGEKLTGVYRVGEGDVLDIKPKGAVSPGST
                     LYTVLAGGLLEYPPLGAPVAVAGMTTDEIAGKLDAELRRRGAASAGLTVNTREYVSHT
                     ILVSGLVESRGTKVLRREAVPLYVIMAEAQPLKEAGLVIVNSHATGRAVKVRLEEIHR
                     EGLLVRPGDVVHVAVSEPEFFYISGKVTEPGEKKFRRGLSLSQAIVASGGLLKAGKYC
                     IVSRDGADGKLITLRVNLKHLAAGVTTDPLLQAGDRLEVF"
     gene            3721..4149
                     /locus_tag="AC159P_19515"
     CDS             3721..4149
                     /locus_tag="AC159P_19515"
                     /inference="COORDINATES: protein motif:HMM:NF018894.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HIR3697684.1"
                     /translation="MSATLTDSGTRLATGPASFKIEFDFFDRSPDGRRKRPRALRAEA
                     PLPVLWSWGPVEQQGVVRNICVGGCFIEAEGEVSLGDKIQFRLSVPGLLHMQMAGMVV
                     RRQRGEGFALRFNKLSTTEQSLLERAVRHLQRHAAPAKKE"
     gene            complement(4171..>4533)
                     /locus_tag="AC159P_19520"
     CDS             complement(4171..>4533)
                     /locus_tag="AC159P_19520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HIR3697685.1"
                     /translation="GGHVEGIMGHRAMRLRFLDLEPEEVFEQAAAGDQRCGDFVKLWH
                     RALAAGTATSVHLDGPGKFFISGPSAGFVQINLLDQYLQEMVKMSPLQGSAFEVISTS
                     DTTALIGAAVSAERAARA"
BASE COUNT          759 a         1572 c         1446 g          756 t
ORIGIN      
        1 tgcacgacat gacgatgatc gaaagtaacg tccggattct catatacgtg ttcccctttc
       61 gccagaggaa cgcgccggac gcaagccgct tgcaaccgaa cactcccgac cacctgtcag
      121 cacgcgccgg acgatgactc ccgcccgcct tcttctactt cgagcaactc cacgtcgaac
      181 gtcagcaacg cgttcgcggg gacgacgccg ggcacgcctc tgtccccgta accgaggtgc
      241 ggggccacgc ggattctcct gcgaccgccg gccctcatcc cctccacccc gtactccaac
      301 ccggcgatga cctgtcggcc acccaacttg aaggactcga ccgcgccccg ctggaaggcg
      361 tcgccccgcc gcagaaagcc gtcgtaacgg atcgtcacgg tgtcaccccg cccggcgctc
      421 gccccttcgc cgagcttgat ctcctcgatg ctgattcctt tacgcattcg ccactgaacc
      481 gtcgagccgg acgacgggcg tcaactctgc gccggccact taccggcaag acagtcacga
      541 aggcccgccg ggcaaggtcg aagttgttgc cacggacgcg gcctccttgc tgacgccggc
      601 gagccggtaa aagctctcgg cctcgcgcgg actgccgaag gctcgcttcg ggatgatgtg
      661 cgcggcggac ggggcggtgt agatgaagac gtactcctcg ttctgttcga cccggtctac
      721 ccccgcccac gtgacgcgcg attcgccgac cgccgtccgc tcgaccagac ccgtttcggt
      781 caaggcgatt cgatgcaccc cgagctgacc cttgtccggt ttctcgtcct tcagaagttc
      841 cctgatcgcg cgccgggtct gacgcctgtc ggagtaccgg atcatacagg cgaggacggg
      901 gaaggcgacg gcgacccacg caaaattcgc gagtgagagg gagaagcccc ccgagccgaa
      961 cgcgcccacc agggagagga cgaggagcag cagcagcagg tagatgtagg ccttgcgcct
     1021 cgcgcgccgg gtgatcggcg acttgtagca ggcgcgccac tggtaggcga agaggtcttc
     1081 ggtggtgatt tcgtactcga cttccatcgg ccactcctcc tcccgagcga cgcgggcggc
     1141 agtcacccgg cgctccagat ttcgtgcgcc ctcaaaatat catccgtcga gtaagcgccc
     1201 tcttcgatcc gtggggcgtg gttgtcgatc aggaactcaa gaaagcccgc gacgaaggcg
     1261 cgctgctccg cgctgcacgc gccgaccaga cggtttcgct ccccgtcctg aatcagatga
     1321 aaaaggaact ggtcgaggta tacctcgccg gggctgccga tggtctccag cgtgatcctg
     1381 acgagggcgg gcatgtagta gacgagaccc tccgccgacg agaagcacag cgggtcccat
     1441 gcaggctgtc ccaactgcgc gaggccgatg gactccacgt cgtgcgccga gagcgtctcg
     1501 tcgtgctcgg cgcactcgca gcagtgccgg tagttaccga agtgggcggg cttcggcacg
     1561 tcgaacaata gtctggctcg cctgtaccaa tccatcgcag cacctttgcc gtgccccgcc
     1621 cgccgccgcc gctcgacgaa acgggcgcgg cctccggcgg tcgagggccg cgccacacga
     1681 ggccgggggg cacgaagaca gatatcttgg tgccccccgg ccttcgcggt caacattctc
     1741 ggcgcgcggc gcgttcgcgg tctcgcccac cgtcttaagc cgcgtcccgt cctcgtcgtc
     1801 gcgcttggcg gcccgcacgc ggcatcaaaa aacctccaac cgatcccccg cctgcaagag
     1861 cgggtcggtg gtgacccccg ccgcgagatg cttcaggttg acccgcaggg tgatgagttt
     1921 gccgtcggcc ccgtcccggc tgacgatgca atacttaccc gccttcagca gcccgccgga
     1981 cgccacgatg gcttgcgaga gagagaggcc gcggcggaat tttttctcgc ccggctccgt
     2041 caccttcccg ctgatgtaga agaactccgg ctcgctgacc gccacgtgga cgacatcgcc
     2101 ggggcgaacc agtaagccct cgcggtgtat ctcctccagc cggactttca ccgccctgcc
     2161 ggtcgcgtgc gagttcacga tcacaaggcc cgcctccttg agcggctgcg cctcggccat
     2221 gatgacgtag agcggcaccg cctcgcgccg caggactttc gtcccacgac tctccaccag
     2281 accgctgacg aggatcgtgt ggctgacgta ctcgcgtgtg ttgacggtca gccccgccga
     2341 cgccgccccg cgccgccgca gctcggcgtc gagcttgccg gcgatctcgt ccgtggtcat
     2401 cccggcgacg gcgacgggcg cgccgagcgg cgggtattcg agcagcccgc ccgccagcac
     2461 ggtgtacaga gtcgagccgg gcgagaccgc acccttcggc ttgatgtcga gcacgtcgcc
     2521 ctcgcccacg cggtagaccc cggtcaactt ttcgccttcg gcggccccgc ccggacgaac
     2581 cggctcacct ccggatactc cgggcgggtc attcggggcc ttcagttcga gcttgagcgg
     2641 gccgctcaaa agcaggatga cgcccgcgtt gttggtgttg cggggggcca gtcgatccgc
     2701 ccgcagcttc gcctcgtacg cctcgtcggt ctggccgagt ttcaggtaag cgtcgctcag
     2761 gttgtggtga atcacggcgt cggacggcgc cagtttgacg gcctgattaa gcgcccctac
     2821 cgcgtcccgg ctccgcccgc cgcgctgata cgcgatgccg aggttattca agacctccgc
     2881 gctcgggccg ccgacgccgt gcgcctgctt gagcaccttc tcggcctcgg cgtacctgcc
     2941 gagataggtg tacagggcgc cgagggccgc gaggctcgcc gtatttttcg ggtggagctg
     3001 gacggatttt tcgaggagcc gcgacgcctc aaggtagctc ccgagttgca ggtgcgcgag
     3061 gctcatgagg tagagctgcc cggcgtcggg ccgctcgtac ttgattgcct gaccgtacga
     3121 agcgacggcc tcttcgaatt tgccgccgcg gtaaaagtcc aggccccttg cgaagaacct
     3181 ctcggcctcc ttgcgaccac cgcgggcctc ctgaccgaat gccgcgtgcg ggaagaggcc
     3241 gatgacgagc aggagcatgg cccccttgat tgacgtggat gttctcgcgg ttgacacttt
     3301 gttgcagcct ccgtggggcg cgcggcccga gtcgtgtgtg gcgcaccgaa ggccgggcca
     3361 ccccggccag acagtccgac ccacttcaag aaattcctaa agcgacacag tttctagagg
     3421 tgaatcgcgc ggacgtgtgg gcggcggagc cgacctcgga ttttccgggc aagccgcggg
     3481 ccgcgccgcg gaaggctgaa acggcgtgac gtgacaccag tttgacaggt cggaatgacg
     3541 gaacggtcag agcagagacc gccgccccga ccgaatatcg gctacggacg ccgccctgcc
     3601 ctccggcacg ggggttgact acccgtcccc gattgtggcc gccggaaggc tggcggcccc
     3661 ggctttacag ttgtcgccgc ctcgcccaac cgaatgacct gaccgaaagg cttgagacac
     3721 atgtcagcga cgttgacgga cagcgggact cgcctggcga ccggcccggc gagtttcaaa
     3781 atcgagttcg atttcttcga ccgcagcccc gacgggaggc gcaagcggcc ccgcgccttg
     3841 agggcggaag cgccgctgcc ggtgttgtgg agttgggggc cggtcgagca gcaaggggtc
     3901 gtccggaaca tctgcgtcgg cgggtgcttc atcgaggcgg agggcgaggt ctctctgggc
     3961 gacaagattc agttccggct gtccgtcccc ggactgctgc acatgcagat ggcggggatg
     4021 gtggtgcgca ggcagcgcgg cgaagggttc gccctccgct tcaacaagct gagcacgacc
     4081 gaacagtcac tgctggagcg cgccgtcagg cacctccaac ggcacgccgc gcccgcgaag
     4141 aaggaataac tcaaccctga acactctctc ctacgcccgc gcggcgcgct ccgcgctcac
     4201 cgcggcgccg atcagggcgg tggtgtcgct ggtcgaaatc acctcgaagg cgctgccctg
     4261 caaagggctc atcttcacca tctcttgcag gtactgatcg agcaaattaa tctgcacgaa
     4321 accggcgctc gggcctgaga tgaagaactt gccggggccg tccagatgga cgctggtcgc
     4381 cgtccccgcc gcgagcgcgc ggtgccacag cttcacgaaa tcgccgcagc gctggtcgcc
     4441 cgccgccgcc tgttcgaaca cctcctccgg ctccaaatcg agaaagcgga gccgcatcgc
     4501 gcggtggccc atgatgcctt ccacgtggcc gcc
//
LOCUS       DBNGOH010001002         1821 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1434083, whole genome shotgun sequence.
ACCESSION   DBNGOH010001002 DBNGOH010000000
VERSION     DBNGOH010001002.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1821)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1821)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1821
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1434083"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1767)
                     /locus_tag="AC159P_19525"
     CDS             complement(<1..1767)
                     /locus_tag="AC159P_19525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015350968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M36 family metallopeptidase"
                     /protein_id="HIR3697686.1"
                     /translation="MLLIFALLLSAAVLPTGSAPASAQSDKSEKAKKPKSFGHKPEPV
                     SVEPAGVRGALTAPDKGKPFDIAVGFLKQNRQRFGLEESDLSELRVTDQYTSRHNGVT
                     HIYLRQEIGGIEVVGADAATNVARDGKLINFHSSFVRGLGRAGRGGPTLSAEQAVESA
                     AKHLGLTLTEPLAAVEAEGGPQNRQTFGTGGISTDPITAKLVYQPVDDGNVRLAWSVE
                     IGELSGEHYWNLRLDAATGEVLAGDDYVDSDDWGANFAPDIERNRRLAAVAAAARPAA
                     ASAAARPFASAALATVNDGSAYNVFALPFESPTDGARTLVTNPADALASPFGWHDTNG
                     AAGPEFTRTRGNNVHAYTDVDANNVADVNSDPDGGPTLQFDFPFDDVTTAPPAYRPAA
                     VTNLFYWNNIVHDVFYQYGFDEISGNFQVNNYGRGPVVAPLGANDDVRAEAQDGSGTN
                     NANFNTNVDGVRPRMQMFVWTYPFSRSVIVNSPAAIAGNYPASGAAFGPAVTAPGITG
                     NVALANDGDGSTSDACQPLVGFPAGQIALLDRGSCDFVVKVKNAQNAGAVAVIVVNNV
                     AGDPVTMGGTDATITIPSVMVSL"
BASE COUNT          263 a          561 c          688 g          309 t
ORIGIN      
        1 cagactgacc atgacggaag ggatcgtgat ggtcgcatca gtgccgccca tcgtgaccgg
       61 gtcgcccgcg acgttgttga cgacgatgac ggcgacggcc cccgcgttct gcgcgttctt
      121 caccttgacg acgaagtcgc acgagccgcg gtcgaggagc gcgatctgac cggcggggaa
      181 gccgacgagc ggctgacagg cgtcgctcgt gctgccgtcg ccgtcgttgg cgagcgcgac
      241 attgcccgtg atgccggggg cggtgaccgc cgggccgaag gcggcgccgc tggccgggta
      301 gttgccggcg atggccgcgg gcgagttgac gatgaccgag cgcgagaagg ggtacgtcca
      361 gacgaacatc tgcatccgcg ggcggacgcc gtcaacgttg gtgttgaagt tggcgttgtt
      421 cgtccccgac ccgtcctgcg cctctgcgcg cacgtcgtcg ttggcgccga gcggcgcgac
      481 gacggggcct ctgccgtagt tgttgacctg gaagttgccg gagatttcgt cgaagccgta
      541 ctggtagaac acgtcgtgga cgatgttgtt ccagtagaaa agattcgtga ccgccgccgg
      601 gcgataggcc gggggcgccg tcgtcacgtc gtcgaagggg aagtcgaact ggagcgtcgg
      661 cccgccgtcg gggtcgctgt tcacgtcggc gacgttgttg gcgtcaacgt ccgtgtaggc
      721 gtggacgttg ttgccgcgcg tgcgcgtgaa ctcggggccg gcggcgccgt tcgtgtcgtg
      781 ccagccgaag ggcgaggcga gcgcgtccgc ggggttggtg acgagcgtgc gagcgccgtc
      841 ggtcggactc tcgaagggga gggcgaagac gttgtaggcc gagccgtcgt tgacggtcgc
      901 cagcgcggcg gaagcgaagg ggcgcgcggc ggcagaggcc gcggcgggcc tggcggcggc
      961 ggccaccgcc gcgaggcgcc ggttgcgctc gatgtcgggg gcgaagttcg cgccccaatc
     1021 gtcgctatcg acgtagtcgt caccggccag cacctcgccg gtcgcggcgt cgagccgcag
     1081 gttccagtag tgctcgccgc tcagctcgcc gatctcgacg ctccacgcca gacggacgtt
     1141 gccgtcgtcc acgggctgat agacgagctt ggcggtgatc gggtcggtgg agatgccgcc
     1201 cgtgccgaag gtctgccggt tctgcgggcc gccctcggcc tcgaccgccg caagcggctc
     1261 ggtgagggtc agcccgaggt gcttcgcggc ggactcgacg gcctgctccg ccgacagcgt
     1321 ggggccgccc cgcccggcgc gaccgagacc gcggacgaag ctgctgtgga agttgatgag
     1381 cttgccgtcg cgcgccacgt tggtcgccgc gtcggcgccg acgacctcga tgccgccgat
     1441 ctcctgcctg agataaatgt gcgtgacgcc gttgtgccgg ctcgtgtact gatccgtcac
     1501 ccgcagctcg gagaggtcgg actcttcaag cccgaagcgc tggcggttct gcttcaagaa
     1561 cccgacggcg atgtcgaagg gcttgccctt gtcgggggcg gtcaaagcgc ctctgacccc
     1621 ggcgggttcg accgacacag gctcgggctt gtggccgaac gacttcggct tctttgcctt
     1681 ctcggacttg tcagattgcg ccgaggcggg ggcggaaccc gtggggagga cagccgcgga
     1741 caggagcagg gcgaagatta acagcaagga aagcttcttg cgtggaggga catttatctt
     1801 caaggcggta tctccttatg g
//
LOCUS       DBNGOH010001003         2266 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1801957, whole genome shotgun sequence.
ACCESSION   DBNGOH010001003 DBNGOH010000000
VERSION     DBNGOH010001003.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2266)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2266)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2266
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1801957"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(122..>2266)
                     /locus_tag="AC159P_19530"
     CDS             complement(122..>2266)
                     /locus_tag="AC159P_19530"
                     /inference="COORDINATES: protein motif:HMM:NF051698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HIR3697687.1"
                     /translation="LTNPSFYDCSGDAVIFDNETCSDPIFPDVSSASETDLRDALTAI
                     IGRFSQYNAGLVYSQTGQLEAVGTPTVRSFATQEFELYFQDSWRWRPNFTVNYGLRWS
                     TSTPVYETKGFQVKPDQSLGEFFRRRVESAEAGVPFNDLITLSLAGKANDRPGFYEQD
                     WNNFAPSVSFAWSPDFGDGFMGRLLGRNGRSVIRGGYRLTYDRIGSQLAVTFDLNNSL
                     GFVSTSGVSANTFNVSDRLAPLFAGPNPQVRGLLTDSGVSIPSSITFPLTRPADEDQR
                     IEQSLDDTLTTPYNHSFNLSYGREIGKGLSFEASYVARYAKNLLASRDIMQLNNIRDP
                     ASGMTWYQASNMLVDLRYAGVPITSVQAIPFFENLFPGLAGTFNVLGTPTALTATQRA
                     YRSIALPSVGGQNVTDYTFRQLQWDDQPIARFNNSFYHPQYAALSVFSTIARSNYNAA
                     QLSVRQRFGADVQFDLNYTYSHSLDNASGLQTATAYGSSGFILNALDPDTNYASSDFD
                     ARHIVNANWLVGLPIGRGKAFFSDMNKFANGILGGWTLTGIFRFNTGLPIVGVPVDLD
                     RWATNWNAQSNGVAVRPVVTSPGDFNGSPNLFADPTAALQSYRDARPGEGGDRNTLRG
                     PGYITLDAGLYKSFTMPWEGHRIQFRWEVYNVTNTQRFDAATIADLTLPQDPFIFGQD
                     ATLDFGRFTSTQTPLNETKAGRVMQFALRYEF"
BASE COUNT          360 a          668 c          864 g          374 t
ORIGIN      
        1 ctctgcgcgc cgccgcgccg cgtttcatcc gcaccggtta atcaaggccg ccgaaaaaaa
       61 ggggccgggg ccgaagcccc ggcccgccgg gaaggtgggt ggcaaagccc tcgcgccgct
      121 tttaaaactc gtagcggagc gcgaactgca tgacgcgccc ggccttggtc tcgttgagcg
      181 gcgtctgcgt cgaggtgaag cgcccgaagt cgagggtcgc gtcctggccg aagatgaacg
      241 ggtcttgcgg cagggtcaga tcggcgatgg tggcggcgtc gaagcgctgc gtgttggtga
      301 cgttgtagac ctcccagcgg aactggatgc ggtggccctc ccacggcatc gtgaacgact
      361 tgtagaggcc ggcgtcgagc gtgatgtagc ccggcccgcg cagcgtgttg cggtcgccgc
      421 cctcgccggg gcgcgcgtcg cgatagctct ggagcgcggc ggtcgggtcg gcgaagaggt
      481 tcggcgagcc gttgaagtcg cccggcgagg tcacgaccgg gcggacggcg acgccgttcg
      541 actgcgcgtt ccagttcgtc gcccagcggt cgagatcgac cggcacgccg acgataggca
      601 gccccgtgtt gaagcggaag atgcctgtca gcgtccagcc gccgaggatg ccgttggcga
      661 acttgttcat gtcggagaag aacgccttgc cgcgcccgat gggcagcccg acgagccagt
      721 tcgcgttgac gatgtggcgc gcgtcgaagt ccgagctggc gtagttcgtg tcggggtcga
      781 gcgcgttgag gatgaacccg gacgagccgt aggcggtcgc cgtctggagg cccgaggcgt
      841 tgtcgagcga gtggctgtag gtgtagttga ggtcgaactg cacgtcggcg ccgaaccgct
      901 ggcggacgct gagctgcgcg gcgttatagt tcgagcgcgc gatggtcgag aagaccgaga
      961 gcgccgcgta ctgcgggtgg tagaagctgt tgttgaagcg tgcgatgggc tggtcgtccc
     1021 actgcaactg gcggaaggtg tagtcggtca cgttctgccc gccgaccgag ggcagcgcga
     1081 tgctgcggta agcccgctgc gtcgccgtca gggccgtcgg cgtgccgagc acgttgaagg
     1141 tgcccgcgag gcccgggaac aggttctcga agaacgggat cgcctgcacc gacgtgatcg
     1201 gcacgcccgc gtagcgcagg tcaaccagca tgttggaggc ctgataccac gtcatgccgg
     1261 acgcggggtc gcggatgttg ttgagctgca tgatgtcgcg cgaggccagg aggttcttcg
     1321 cgtagcgcgc gacgtaggaa gcctcgaacg aaagaccctt gccgatctcg cgcccgtagg
     1381 agaggttgaa gctgtggttg tagggcgtcg tcagcgtgtc gtcgagcgac tgctcgatgc
     1441 gctgatcctc gtccgccggg cgggtcagcg ggaaggtgat cgagctgggg atgctgacgc
     1501 cgctgtcggt gagcaggccg cgcacctgcg ggttcggccc cgcgaagagc ggcgcgagcc
     1561 ggtcgctcac gttgaaggtg ttggccgaga cgccgctcgt cgagacgaag ccgagcgagt
     1621 tgttgaggtc gaaggtgacg gcgagctggc tgccgatgcg gtcgtaggtc aggcggtagc
     1681 cgccgcggat gacgctgcgc ccgttgcgcc cgagcagccg ccccatgaag ccgtcgccga
     1741 agtcgggcga ccacgcgaac gagacggacg gcgcgaagtt gttccagtcc tgctcgtaga
     1801 agccgggccg gtcgttggcc ttgcccgcga ggctgagcgt aatcaggtcg ttgaagggca
     1861 cgccggcctc cgcgctctcg acgcggcgcc ggaagaactc gcccaggctc tggtccggct
     1921 tgacctggaa gcctttcgtc tcgtagaccg gcgtcgaggt ggaccagcgc aggccgtagt
     1981 tgaccgtgaa gttcgggcgc cagcgccacg agtcctggaa gtagagttcg aactcctgcg
     2041 tggcgaacga gcgcacggtg ggcgtgccga ccgcctcgag ctgcccggtc tggctgtaga
     2101 cgaggcccgc gttgtactgc gagaagcgcc cgatgatcgc ggtcagcgcg tcgcgcaggt
     2161 cggtctccga cgccgaggac acgtccggga agatcgggtc ggagcaggtc tcgttgtcga
     2221 agatgacggc gtcgccggag cagtcgtaga acgaggggtt ggtcag
//
LOCUS       DBNGOH010001004         4830 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1617343, whole genome shotgun sequence.
ACCESSION   DBNGOH010001004 DBNGOH010000000
VERSION     DBNGOH010001004.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4830)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4830)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4830
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1617343"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..426)
                     /locus_tag="AC159P_19535"
     CDS             complement(<1..426)
                     /locus_tag="AC159P_19535"
                     /inference="COORDINATES: protein motif:HMM:NF024637.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HIR3697688.1"
                     /translation="MTRCQHCGQQVEEGKQFCPYCGKPAGGEAAEARQAAGGAREGAA
                     RPRASDERPAPPRPEPMSGFGQGRGEADSSRRAIYIIVAGVALALTALLLYFALRPAP
                     PRAEPRLEGGIRDGSPEFAQVKERLVVEFDPDQTASESPR"
     gene            complement(458..2836)
                     /locus_tag="AC159P_19540"
     CDS             complement(458..2836)
                     /locus_tag="AC159P_19540"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR3697689.1"
                     /translation="MKETMREEQTNIRLDVRTKLLVMLAVLSLPLLIISLVQLRSYRR
                     NLNEQAAAMARVESAAAAGAVEHFVETHSQLVGVGAEDVPLPSSEAENLYARLRRQAV
                     QGMGSAVTVFDARQRALAGPSQAAHAPEDLREDVRQQRWSDGVSRLTSVRRAGATGWL
                     VAVGVPLPEDTPAGRSILILAVTWALALLASCLLAVWAVGRFTKPLIKLTESAATLGE
                     GHLAERAAVESGDEVGALARSLNAMAASLETKFEDVKRQSAFIGEVLDSLPLGVVVLD
                     AHLVVRKVNAAFAAMAGGEAAGLPGRGLYDAAPGLAVMGEIVEEVRRTRRAFVNYGLA
                     LELSAQDRAGREGTKFWDVIVWPLREQSDGRGDLILILSEVSKRVRAERLATAAFAAE
                     KSRAAELESVIRQMVEGVVIVDSSGRYRVNPAAARILGREPGEFRDGITALVVDMALR
                     DMQGRVQMLEETPISRALVGGETVAGERCKFVRGDGEERVLSVGATPLNGEQGRLEGA
                     VVVFRDITEEVERHEELVAAYDRLREHDRLKSAFVANMSHELRTPLNVIIGLCQLLER
                     DPQLPLAPLQSEAVVRMERNAHALLDLVNDMLDYSRLEAGRSALRIESVDVAEVINEV
                     AEEYGEEATEKKIKFGIKVSPEIGHVRTDRQKLTQVVECLVSNALKFTSSGTVVISAA
                     PVGEDKWYIEVSDTGIGISSDALTYIFDGFRQVDDRLARSYSGVGLGLAITRRIVELL
                     EGEITVESKENEGSRFRITWPREAKQRTGTGSLVGQGGLALVEALKREANLR"
     gene            complement(2904..3293)
                     /locus_tag="AC159P_19545"
     CDS             complement(2904..3293)
                     /locus_tag="AC159P_19545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020615095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3697690.1"
                     /translation="MSDNNGAALTVLVVEDFEDNRFMMRRLLEMSGYRVIEAVNGRQA
                     VEAAISERPDLILMDLSLPMLDGLAATRRIREAEGLGHVPIVAVSAHDSADFHTEALA
                     AGCNEYVTKPIDFDHLVRILDGLVSRG"
     gene            3601..4176
                     /locus_tag="AC159P_19550"
     CDS             3601..4176
                     /locus_tag="AC159P_19550"
                     /EC_number="2.7.13.3"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HIR3697691.1"
                     /translation="MNGLKLYATFLKKRMEKGERPADELETIGKIIAGLERAATDMTV
                     LVRYGRPLDVRPQPRVDLARLLAEAAGDVAVRAEGEGFEGDFDAPLLGEALQIITAGA
                     GVGNGRNADAAAQDSPRIRLRRDDAGAAPEAVIEWGGGARAGVPEDVFDTFAGGQGLR
                     LALAARIIRSHGGEVGQESGVLHARLPLSKV"
     gene            4273..>4830
                     /locus_tag="AC159P_19555"
     CDS             4273..>4830
                     /locus_tag="AC159P_19555"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HIR3697692.1"
                     /translation="MERKDVRILVVDDEPVMADSLKQNLVEEGYTVDTASDGTGAIEM
                     FDRGGHHIAVCDLQLPDMDGLEVLKHIKDTRPSTEVIVVTGYGSVAKAVEATKAGAFW
                     FMEKPFDFEVLQPLVERALQRYELMSETENMRRQLSTRTEYFNIIGASKQMQTIYETI
                     ESVAKSDANVLIVGESGTGKELIANA"
BASE COUNT          791 a         1688 c         1562 g          789 t
ORIGIN      
        1 gcgcggcgac tcgctcgcgg tctggtcggg gtcgaactcg acgacgaggc gctccttgac
       61 ctgcgcgaac tcgggcgagc cgtcgcggat gcctccttcg agtcgcggct cggcgcgcgg
      121 cggcgcgggg cgcagcgcga agtacagcag cagcgccgtc agcgcgaggg cgacgccggc
      181 gacgatgata tagatcgcgc gccgtgagct gtcggcttcg ccgcgcccct gcccgaagcc
      241 cgacatcggc tccgggcgcg gcggagcagg ccgctcgtcg gacgcccgcg gacgcgccgc
      301 gccctcgcgc gcgccgcccg ccgcctgccg cgcttcggcg gcctccccgc ccgcgggctt
      361 accgcagtac gggcagaact gtttcccttc ctcgacctgc tggccgcagt gctggcaccg
      421 tgtcacggct ggttcgactc ctttcggcgt gattttttta gcggaggttc gcctcgcgct
      481 tgagcgcttc gacgagggcg aggccgccct gaccgacgag agagccggtg cccgtgcgct
      541 gcttggcctc gcgcggccag gtgatgcgga agcggctgcc ctcgttctcc ttgctctcga
      601 ccgtgatctc gccttcgagc agttcgacga tgcgccgggt gatggcgagt cccaaaccga
      661 cgccgctgta gctgcgcgcg agccggtcgt ccacctgccg gaagccgtcg aagatgtagg
      721 tcagcgcgtc gctcgaaatg ccgatccccg tgtcagagac ctcaatatac cacttgtctt
      781 cgccaacggg cgcggcgctg atgacgaccg tgccggagga ggtgaacttc aacgcgttgc
      841 tgacgaggca ctcgacgacc tgcgtcagct tctgccggtc ggtgcgcacg tgcccgatct
      901 cgggcgagac cttgatgccg aacttgatct tcttctcggt cgcctcttcg ccgtactctt
      961 cggcgacctc gttgatgacc tcggccacgt ccacgctctc gatgcgcaga gccgagcggc
     1021 ccgcttcgag ccgcgaatag tcgagcatgt cgttgacgag gtctaagagc gcgtgcgcgt
     1081 tgcgctccat ccgcacgacg gcctcggact gcaagggcgc gagcgggagc tgcgggtcgc
     1141 gctcaagcaa ctggcacagc ccgatgatga cgttcaaggg ggtgcgcagc tcgtggctca
     1201 tgttggcgac gaaggccgac ttgagccggt cgtgctcgcg cagccggtcg taggccgcga
     1261 ccagctcctc gtgccgctcg acctcctccg tgatgtcgcg gaagacgacg acggcgcctt
     1321 cgagcctgcc ctgctcgccg ttgagcgggg tggcgccgac cgacagcacg cgctcctcgc
     1381 cgtcgccgcg gacgaacttg cagcgctcgc ccgcgaccgt ctcgccgccc acgagcgcgc
     1441 ggctgatcgg cgtctcttcc agcatctgca cgcgcccctg catgtcgcgc agcgccatgt
     1501 ccacgacgag cgccgtgatg ccgtcgcgga actcgcccgg ctcgcgcccg aggatgcgcg
     1561 ccgccgccgg gttgacgcgg tagcgccccg acgagtccac gatcacgacg ccctcgacca
     1621 tctgccggat gacgctttcg agttccgccg cgcgcgattt ttcggcggcg aaggcggcgg
     1681 tcgccagccg ctcggcccgc acgcgcttcg agacctccga caggatgagg attagatcgc
     1741 cgcgcccgtc gctttgttcc ctcaggggcc acacgatcac gtcccagaac ttggtgccct
     1801 cgcgccccgc gcggtcctgc gccgagagtt cgagcgcgag gccgtagttg acgaacgcgc
     1861 gccgggtgcg cctgacctcc tcgacgatct cgcccatcac ggccagtccc ggggcggcgt
     1921 cgtacaggcc gcggcccggc agccccgccg cctcgccgcc cgccatcgcg gcgaaggccg
     1981 cgttgacctt gcgcacgacc aggtgcgcgt cgagcacgac gacgccgagc ggcaggctgt
     2041 cgagcacctc gccgatgaag gcgctctgcc gcttcacgtc ttcgaacttc gtttcgaggc
     2101 tcgccgccat cgcgttgagg ctccgcgcga gcgcgcccac ctcgtcgccc gactcgaccg
     2161 ccgcccgctc ggcgaggtgg ccctcgccga gcgtcgccgc cgattccgtg agcttgatga
     2221 gaggcttcgt gaaccggccc accgcccaca ccgcgagcag gcacgaagcc aagagcgcca
     2281 gcgcccacgt cacggcgagg atcaggatcg agcggcccgc cggagtgtct tcgggcagcg
     2341 gcacgccgac ggcgacgagc cagcccgtcg cgcccgcgcg ccgcacgctc gtcagacgcg
     2401 acacgccgtc gctccaacgc tgctggcgca cgtcctcgcg caaatcttcc ggcgcgtgcg
     2461 cggcctgcga cggcccggcc agcgcgcgct gccgcgcgtc gaacacggtg acggcgctgc
     2521 ccatgccctg cacagcctgc cgcctcagac gcgcgtaaag attttcggct tcggacgaag
     2581 gcagcggaac gtcttcggcg ccgacgccga cgagttggga atgcgtctcg acgaagtgct
     2641 cgaccgcgcc cgcggctgcc gccgactcga cgcgcgccat cgccgccgcc tgctcgttca
     2701 aattccggcg gtagctcctg agctgcacga ggctgatgat gagcagcggc agagacagca
     2761 cggccagcat caccagcagc ttggtgcgca cgtcgagccg gatgttcgtc tgctcttccc
     2821 gcatcgtctc tttcaaaaga gggactagag actagaagct agttacaacc actctgttcc
     2881 aacaagtctc aagcctcaag tctctagcct ctgctgacca gcccatccag tatccgcacg
     2941 aggtggtcga agtcgatggg cttggtgacg tactcgttgc agcccgcggc gagggcctcc
     3001 gtgtggaagt ccgccgagtc gtgcgccgag acggcgacga tgggcacgtg gccaagccct
     3061 tccgcctcgc ggatgcgccg cgtggcggcg aggccgtcga gcatcggcag gctcaagtcc
     3121 atcaggatca ggtctgggcg ctcgctgatg gccgcctcga ccgcctgtct cccgttgacg
     3181 gcctcgatca cgcggtagcc gctcatctcc aacaaccttc tcatcatgaa gcggttgtcc
     3241 tcgaagtcct cgaccacgag gacggtcagc gccgcaccgt tattgtccga catgaaaacc
     3301 tactctcccg gctcccgttc aactttggaa cgcccaggca gaaagcgcac cgtacgacta
     3361 gcaaacagtt gccatttttg ttaagatgcc gcatcgaaac gcaacacaaa cgacatctgt
     3421 ttttcgtgta attcgtacag gctggaaggc ttagcgggcg cccccctgcg ccgtccattc
     3481 tccctgagtt gaggtctgcg ggtcattatg aagccgttaa aatcagagtt acaagatgcc
     3541 gagaccttgc gccagctcgg gagcgcgtcc gttcagatcg tgcacgacct taaaaaccaa
     3601 ttgaacggtc tcaaactcta tgccactttc ctcaagaaac ggatggagaa gggcgagcgc
     3661 ccggcggacg agcttgagac catcggcaaa atcatcgcgg gcctcgaacg ggcggccacg
     3721 gacatgaccg tgcttgtccg ctacgggcgc ccgctcgacg tgcgcccgca gccgcgtgta
     3781 gacctcgcgc ggctgctcgc cgaggcggcg ggcgacgtgg ccgtccgggc cgaaggcgaa
     3841 ggcttcgaag gcgacttcga cgcgcccctg ctcggcgagg ctttgcagat tatcaccgcc
     3901 ggcgccggcg tcggcaacgg gcgcaacgcc gacgccgcgg cacaggactc tccccgaatc
     3961 cgcctgcgcc gcgacgacgc gggggcggcg cccgaggcgg tcatcgaatg gggaggcggc
     4021 gcgcgggcgg gcgtccccga agatgtcttc gacacgttcg ccggcgggca gggcctgcgc
     4081 ctcgcgctcg ccgcgcgcat catccgctct cacggcggcg aagtcgggca ggagtcgggc
     4141 gtcttgcacg cccgtctacc actatccaaa gtctgaagtc tgaagtcaga aggcagaacc
     4201 ggcacacttt tgtctttagt tctgccttca gacttcagac ttcagacttc attatcgggg
     4261 aggattagaa gagtggaacg taaggacgtg cgtattctgg tggtggacga cgagccggtg
     4321 atggccgact cgctgaagca gaatctggtg gaggagggct acacggtgga cacggcgtcg
     4381 gacggcaccg gggccatcga gatgttcgac cgcggcggcc accacatcgc cgtctgcgac
     4441 ctgcaactgc ccgacatgga cggactcgaa gtcctcaagc acatcaagga cacgcgtccc
     4501 tcgaccgagg tcatcgtcgt caccggctac ggctcggtcg ccaaggcggt cgaggcgacg
     4561 aaggccggcg ccttctggtt catggagaag cccttcgact tcgaagtcct ccagccgctg
     4621 gtcgagcgcg cccttcaacg ctacgagctg atgagcgaga ccgagaacat gcgccgccag
     4681 ctctcgacgc gcacagagta cttcaacatc atcggcgcct cgaagcagat gcagaccatc
     4741 tacgagacca tcgagtcggt cgccaagtcg gacgccaacg tgctcatcgt cggcgaatcg
     4801 ggcaccggca aggagctgat cgccaacgcg
//
LOCUS       DBNGOH010001005         8515 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_700046, whole genome shotgun sequence.
ACCESSION   DBNGOH010001005 DBNGOH010000000
VERSION     DBNGOH010001005.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8515)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8515)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8515
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_700046"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(55..1224)
                     /gene="aroC"
                     /locus_tag="AC159P_19560"
     CDS             complement(55..1224)
                     /gene="aroC"
                     /locus_tag="AC159P_19560"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HIR3697693.1"
                     /translation="MFRFTTAGESHGRALVAIVEGMPAGLAVDVESINRELERRQWGY
                     GRGGRMKIERDRAEILSGVRHGLTLGSPVALTVENKDWKNWTEVMAAEALEVAPEKSR
                     RVKRPRPGHADLAGGQKFGTRDLRDILERASARETTMRVACGALARQLLAAFGVELKS
                     HVVQLGGVPVEPLEVSWEGVAAIPEDAPLRCADAEAQARMVALVDEKRREGDTLGGIF
                     EVVARGVVPGLGSHTSWEEKLDGRLARAVMSIPAVKGVSIGAGVEAASLPGSQVHDEI
                     TYDDGAKAFARRTNRAGGLEGGITNGEELRVRGFLKPISTLRRALRSVDIDTKEEEAA
                     AFERSDVTAVPAAGVIGEAMVALVLAGAMREKFGGDHVAEMRRNFEGYRAQLREY"
     gene            complement(1310..2872)
                     /locus_tag="AC159P_19565"
     CDS             complement(1310..2872)
                     /locus_tag="AC159P_19565"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HIR3697694.1"
                     /translation="MLIKSEQDEIQTFLADASNLAGGHASRVLVPESAEEVATALAQA
                     TAEGTPVTVAGAGTGIVGGRVPQGGVVLSTHRLDRIKWIVRDDTGGGRATVEAGVVLA
                     DFQRAVKTERLLYPPDPTEWSCFMGGTVATNASGARTFKYGPTRAYVRRLKVALATGD
                     LVDLSRGDIEADDDGTIRIPLGGGRKIEARLPTYRMPETRKHAAGYYVAEGMDAVDLF
                     IGSEGTLGVVTEAEVALLPQPEGVLSGIVFFEAEQDLLAFVAEARARSLKAREHSLKA
                     RDLSSTAGAASGLTPSAAARLDARALEYFDDEALHFLRERYPLVPVGAAGAIFFEQEI
                     TDESEEALMGEWLSLLEAHGALLDDSWFGTNEHDRAEMSAFRHQLPVMVNEWLARHGQ
                     RKVSTDMAVPDAHFAAMLGFYKETLRRARLKYVTFGHIGDNHVHVNIMPCDDIEQIAA
                     RDIYAEFIRRAVSLGGTISAEHGVGKIKREYLRALYGDEHLREMAELKRAFDPSLILG
                     RGNIFPEEFLTR"
     gene            complement(2942..3478)
                     /locus_tag="AC159P_19570"
     CDS             complement(2942..3478)
                     /locus_tag="AC159P_19570"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3697695.1"
                     /translation="MIATLETERLILREWTPADAGVIYEICSDPEVMRHIGDGSRWET
                     FERARLWMGRVRAAYREQGFGRYAVVEKESGRVVGSCGFGKANGPAGVDFGYVFARSV
                     WGRGYATEAGRACLRYGFEQVGFAEVVADTDIENHASRRVLEKLGFEFRGLKRNEGDD
                     VDSTFYVARNPSLPQRPD"
     gene            complement(3482..5470)
                     /gene="uvrB"
                     /locus_tag="AC159P_19575"
     CDS             complement(3482..5470)
                     /gene="uvrB"
                     /locus_tag="AC159P_19575"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013568338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HIR3697696.1"
                     /translation="MAFKLRSDFEPQGDQPQAIDALVRGLNDGAKDQVLLGITGSGKT
                     FSVASVIERTQRPTLVIAHNKTLAAQLYQEFKSFFPENSVEYFVSYYDYYQPEAYVPA
                     SDTYIEKEATINEEIDRLRLSATRALFERRDVIVVASVSCIYGLGDPDAYYGMLLFVE
                     PGMKVTREALLQKLVELQYERSDIEFDRGSFRVRGDIVEVYPSYQDQAYRIELWGDEV
                     DSISTIDPLLGEVLHKHTSRLPIYPKTHYVMPKSTIKRALKTIREELDWWEPKLIQEG
                     KLVEAQRLHQRTMYDLEMIKEMGFCRGIENYSRHLSGKAPNEPPPTLLDYLPRESMIV
                     IDESHQTIPQLRAMFKGDQSRKRTLVEYGFRLPSAMDNRPLNFEEFEGRTGQVIYVSA
                     TPGPYELTKTGGEVVEQIIRPTGLTDPVVVVRPVKGQIDDLLEECRVRAERNERVLVT
                     TLTKKMAEDLTEYFAEVGVRVRYLHSDIETLERIKILRDLRRGEFDVLVGINLLREGL
                     DLPEVSLVAILDADKEGFLRSESSLIQTMGRAARNAGGTAILYADNMTDSMRRAIEET
                     ERRRRIQQDYNEEHGITPRTIIKPVEATLVTAYEADYFKVPLELEAFEEYSKDKIEST
                     IARLEAEMRHAAKQLEFERAAELRDRIKYLREREIQMA"
     gene            complement(5593..>8515)
                     /locus_tag="AC159P_19580"
     CDS             complement(5593..>8515)
                     /locus_tag="AC159P_19580"
                     /inference="COORDINATES: protein motif:HMM:NF025030.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HIR3697697.1"
                     /translation="ETNAPATRTNAPATTAVPGPSFGATLLRNWPLALIGALLVVGLG
                     LFFAARRSSATSRAPHAPEEAKPAPVAEAKPAAIEEAKPAATTEAKPLAVEPAKPLVP
                     VVKAEEESKAKPGRQQTDRKKHKKAGKAKLREAPPAPAVVQEETPALVSADMPATTPV
                     VESLTLAPLVAASALAGAAATDEETVDETTKAEPLTAEPEPRAVEETFVEEEAAAAQE
                     AVAEDVVLEEAPVEEVVAEESAAEEAAVEEAVVEEAAAEDVVVEEATVEAATVEDAAI
                     ETTVGEAGAVGETVVEEAADAEPPVVEHSTEARFAEEAEAADAVADLPVGDVAEDVAV
                     ETAEVGTAQAGEVVTAVEAVASAEAVASPDAETDSTVAADAESVQAETKRLLDGERYD
                     RGVLRSADMMGRQMIAAELLSALAGRNAERRGRAGAAFIEHGFFNEAAHDLHYAEAPA
                     ERAAAARSLALAGDRAATSHLVAALEDNAIDVRRAAVEALADLRDPAAVGPLEDLRER
                     QKKQREKLPRRTLQNAIEICREGTADAAPPFVAPAAETASVGQAPDVNVEGQRGEESF
                     AAEASAVEEERAAEQSEAAASAETEIVISPEGDAGVRVEPKEVEPSIEDAAAWFELVA
                     AGHAAAVEETRGEAAPVTESPVVEAAPFVDERAAQPVRVEEAGATDAESYDLPTEVEA
                     KALEPASAPGEASEDWIELDVEESSFAPVAPFEADAAPAEFADAAAYDAAPVEIEARE
                     PEQPFAGATRDVPQAGAFGRGEAPPPRAAEEKGIEQFDELSTVPRAIQQRITSADTGD
                     RAAALADLSRLDPDEAFRHICAAFDDDAREVRNAAARALFDLNPDRADSFTRALRQST
                     AERRRHIGAAINNSGLAAEAIGQLTGESRDKTYEAFSLLFLMAKAGEVQPLLRAIESH
                     PNNEVRLAVVKLLALSGQKEILPAFRRLAVRGSLPTEVRSAVMEAIYQISSGQDATST
                     V"
BASE COUNT         1211 a         2940 c         2838 g         1526 t
ORIGIN      
        1 gccgggctaa actcaactgc cgctacgcgg cggattcgaa catcgcggcg cggctcagta
       61 ttcgcgcaac tgcgcgcggt agccttcgaa gttgcggcgc atctccgcga cgtggtcgcc
      121 gccgaacttc tcgcgcatcg cgcccgcgag cacgagcgcg accatcgcct cgccgatgac
      181 gcccgccgcc ggcaccgccg tcacgtccga gcgctcgaag gccgccgcct cctcttcctt
      241 cgtgtcgatg tccaccgacc gcagcgcgcg ccgcagggtc gagatgggtt tgaggaagcc
      301 gcgcacgcgc aactcctcgc cgttcgtgat gccgccttcg aggccgcccg cgcggttcgt
      361 gcgccgcgcg aacgccttcg cgccgtcgtc gtacgtgatc tcgtcgtgga cttgcgagcc
      421 gggcaaggaa gccgcctcga cgcccgcgcc gatgctgacg cccttcaccg ccgggatcga
      481 catcacggcg cgggccagcc gcccgtccag cttctcctcc caggacgtgt gcgaccccag
      541 ccccggcacc accccgcgcg ccaccacttc gaagatgccg ccgagcgtgt ccccttcacg
      601 acgcttctcg tcaaccaaag cgaccatgcg cgcctgcgcc tccgcgtccg cgcaacgcag
      661 cggcgcgtct tccggtatcg ccgccacgcc ctcccacgaa acctccaaag gctccacagg
      721 cacgccgccg agctgcacga cgtgactctt cagctctact ccgaacgccg ccagcagttg
      781 ccgcgccagc gccccgcacg ccacgcgcat cgtcgtctcg cgcgccgacg ccctttcgag
      841 gatgtcgcgc aggtcgcgcg tcccgaactt ctgcccgccc gctaagtccg cgtggccggg
      901 ccgcggcctc ttcacccgcc gcgacttctc cggcgccacc tccaacgcct ccgccgccat
      961 cacctccgtc cagttcttcc aatccttgtt ctccaccgtc aacgcgacgg gcgaacccag
     1021 cgtcagcccg tgcctgacgc cgctcagtat ctccgcgcgg tcgcgctcga tcttcattcg
     1081 cccgccccgc ccgtaccccc actgccgccg ctcaagctcg cggttgatcg actcgacatc
     1141 gaccgccagc ccggcgggca ttccttcgac gatagcgacc aacgcgcgac cgtgtgattc
     1201 tcctgcggtg gtgaacctga acataagaaa gaagtgacga gcgacaagcg acgagtaaca
     1261 agcgacgagc gacgagcgaa cggcattgta gctcgtcgct cgccacccgt catcgtgtaa
     1321 gaaactcttc cgggaagatg tttccgcgac ccagaatcaa cgaaggatcg aaggcgcgct
     1381 tcagctccgc catctcgcgc agatgttcgt cgccgtagag ggcgcgcagg tattcgcgtt
     1441 tgattttgcc gacgccgtgc tcggcggaga tggtgccgcc gagggagact gcgcggcgga
     1501 tgaactcggc gtagatgtcg cgcgcggcga tctgctctat gtcgtcgcac ggcatgatgt
     1561 tgacgtggac gtggttgtcg ccgatgtggc cgaaggtgac gtacttgagg cgcgcgcggc
     1621 gcaaagtctc tttgtagaag ccgagcatcg cggcgaagtg cgcgtcgggc acggccatgt
     1681 cggtcgagac cttgcgctgg ccgtggcgcg cgagccattc gttgaccatc acgggcagtt
     1741 ggtggcggaa ggcgctcatc tccgcgcggt cgtgctcgtt cgtgccgaac cacgagtcgt
     1801 ccagcagcgc cccgtgcgct tcgagcagcg acagccactc gcccatcagc gcctcttccg
     1861 actcgtccgt gatctcctgc tcgaagaaga tggcgcccgc cgcgcccacg ggcacgagcg
     1921 ggtaacgctc gcgcaggaaa tggagcgcct cgtcgtcgaa gtattcgagg gcgcgcgcgt
     1981 cgagacgcgc ggcggctgat ggagtcaagc cggaagccgc gccggcggtc gatgagaggt
     2041 cgcgtgcttt caatgagtgc tcacgcgctt tcaatgaacg cgcgcgtgcc tcggcgacga
     2101 aggcgaggag gtcttgctcg gcctcgaaga agacgatgcc gctcagcacg ccttcgggtt
     2161 gcgggagcag tgcgacctcg gcctccgtga cgacgccgag cgtgccctcg cttccgatga
     2221 agagatcgac cgcgtccatc ccttcggcga cgtagtagcc cgcggcgtgc ttgcgcgtct
     2281 cgggcatgcg gtaggtcggc agccgcgcct cgatcttgcg cccgccgccg agcgggatcc
     2341 tgatcgtgcc gtcgtcgtcc gcttcgatgt cgccgcggct gagatcgacg aggtcgccgg
     2401 tcgccagcgc gaccttcagt cgccgcacgt aggcgcgggt cgggccgtac ttgaacgtgc
     2461 gcgcgcccga agcgttggtc gccaccgtgc ctcccatgaa gcagctccac tcggtcgggt
     2521 cgggcggata gagcagtcgc tcggtcttga ccgcgcgctg aaagtccgcg agcacgacgc
     2581 ccgcctcgac ggtcgcgcga ccgccgcccg tgtcatccct gacgatccac ttgatgcggt
     2641 cgagcctgtg tgtcgagagc acgacgccgc cctgcggcac gcgcccgccg acgatccccg
     2701 tgcccgcgcc cgcgaccgtg acgggcgtgc cttccgcggt cgcctgcgcg agcgccgtcg
     2761 ccacctcttc ggcactctcg gggacgagca cgcgcgaggc gtgaccgccc gcgaggttgc
     2821 tggcgtcggc gaggaaggtc tggatttcgt cctgctcgga tttgatgagc atgaagtttc
     2881 accgaacgga ctcgcgtgcg gcgtgcgcgg agttgcgtca gccgcgccgt cgcggtcgaa
     2941 gtcagtcggg ccgttgcggc agcgaagggt tgcgcgcgac gtagaaggtc gagtccacgt
     3001 cgtccccctc gttgcgcttc aggccgcgga actcgaagcc gagcttttcg agcacgcgcc
     3061 gcgacgcgtg gttctcgatg tccgtgtcgg ccacgacctc tgcgaacccg acctgctcga
     3121 agccgtagcg caggcacgcg cgccccgcct cggtcgcgta gccgcgcccc cacacggagc
     3181 gcgcgaagac gtagccgaag tccacgcccg ccggcccgtt cgccttgccg aagccgcaac
     3241 tgccgaccac gcgcccgctc tccttctcga ccaccgcgta gcgcccgaag ccctgctccc
     3301 tgtacgccgc cctcacgcgc cccatccaca ggcgcgcgcg ctcgaacgtc tcccacctac
     3361 tcccgtcgcc gatgtgccgc atcacctccg ggtcgctgca tatctcgtag atcacgcccg
     3421 cgtccgccgg cgtccactcg cggaggatca gtcgttcggt ttcgagagtc gcgatcatac
     3481 ttcaggccat ctgaatctcg cgctcgcgca ggtacttgat gcggtcgcgc agctcggcgg
     3541 cgcgttcgaa ttcgagctgt ttggcggcgt ggcgcatctc ggcttcgagg cgggcgatgg
     3601 tcgattcgat cttgtccttc gagtactctt cgaaggcttc gagttcgagc gggactttga
     3661 agtagtcggc ctcgtaggcg gtgacgagcg tggcctcgac gggcttgatg atggtccgcg
     3721 gggtgatgcc gtgctcctcg ttgtagtcct gctggatgcg gcggcggcgc tcggtctctt
     3781 cgatggcgcg gcgcatcgag tcggtcatgt tgtcggcgta gaggatggcg gtgccgcccg
     3841 cgttgcgcgc cgcgcggccc atcgtctgga tgagcgagga ctcggaacgc aggaagcctt
     3901 ccttgtcggc gtcgaggatg gcgaccagag agacctccgg cagatcgagt ccttcgcgca
     3961 aaaggttgat gccgacgagc acgtcgaact cgccgcgccg caggtcgcgc aggattttga
     4021 tccgctccag cgtctcgatg tccgagtgca ggtaacgaac ccggacgccg acctcggcga
     4081 agtattcggt gaggtcttcg gccatcttct tcgtcagcgt ggtgacgagc acacgctcgt
     4141 tgcgctcggc gcggactcgg cactcttcca agaggtcgtc gatctgtcct ttgacggggc
     4201 ggacgacgac gaccgggtcg gtcagtcccg tggggcggat gatctgctcg acgacctcgc
     4261 cgccggtctt cgtcagctcg tacgggccgg gcgtcgcgga gacgtagatg acctgaccgg
     4321 tgcgcccctc gaactcttcg aagttgagcg ggcggttgtc catcgccgag gggaggcgga
     4381 agccgtactc gacgagtgtg cgcttgcgcg actggtcgcc cttgaacatg gcgcgcagtt
     4441 gcgggatggt ctggtgcgac tcgtcgatga cgatcatgga ttcgcgcggc aggtagtcga
     4501 gcagcgtcgg cggcggctcg ttcggcgcct tgcccgaaag gtggcgcgag tagttctcga
     4561 tgccgcggca gaagcccatc tccttgatca tctcaaggtc gtacatcgtc cgctgatgaa
     4621 ggcgctgcgc ctcgacgagc ttgccctcct gaatcagctt cggctcccac cagtcgagtt
     4681 cttcgcggat ggtcttgagc gcgcgcttga tggtgctctt cggcatcacg tagtgcgtct
     4741 tcgggtagat cggcagacgc gacgtgtgct tgtggaggac ttcgcccagg agcgggtcga
     4801 tggtcgagat ggaatcgact tcgtcgcccc acagctcgat gcggtaggcc tggtcttgat
     4861 agctcgggta gacctcgacg atatcgccgc ggacgcggaa gctgccgcgg tcgaactcga
     4921 tgtcggagcg ctcgtactgc agctcgacca gtttttggag cagcgcctcg cgcgtcacct
     4981 tcatgcccgg ctcgacgaag agcagcatgc cgtagtaggc gtccgggtcg cccaacccgt
     5041 agatgcacga gacggaggcg acgacgatca catcgcgccg ctcgaacagc gcgcgcgtcg
     5101 cggacaggcg cagccggtcg atctcctcgt tgatggtcgc ctctttctcg atgtacgtgt
     5161 ccgaggcggg gacgtaagcc tcgggctggt agtagtcgta gtaggagacg aaatactcga
     5221 cggagttctc ggggaagaag ctcttgaact cctgatagag ctgcgcggcc agcgtcttgt
     5281 tgtgcgcgat gacgagcgtg gggcgctgcg tgcgctcgat gacggaggcg acgctgaagg
     5341 tcttgcccga gccggtgatg ccgagcagga cttgatcctt cgcgccgtcg ttcaggccgc
     5401 gcacgagcgc gtcaatcgcc tgcggctgat ccccctgcgg ttcgaagtcg gaacggagct
     5461 tgaatgccat agccggtaaa tagtaaatgg taaatagtga atagacaaaa gccgtaaacc
     5521 gtaaaccgtg aaccgtgata agagagaaca ggtcttctct tatcacggtt cacggtttac
     5581 ggtttacggc ttttaaactg tggacgtggc gtcctggccg ctgctgatct ggtagatggc
     5641 ctccatgacg gcggagcgga cttcggtcgg gagcgagccg cgcaccgcga gacgacggaa
     5701 ggcggggagg atttccttct ggccggagag cgcgagcagc ttgacgacgg ccagacgcac
     5761 ctcgttgttc gggtgcgact cgatggcgcg cagcagcggc tgcacctcgc ccgccttcgc
     5821 catcaggaag aggagcgaga aggcttcgta agtcttatcg cggctctcgc cggtgagctg
     5881 gccgatggcc tctgcggcga ggccggagtt gttgatggcg gcgccgatgt ggcggcgtcg
     5941 ctcggcggtg gactggcgca gcgcgcgcgt gaacgaatcg gcgcggtcgg ggttgaggtc
     6001 gaagagggcg cgcgcggcgg cgtttcgcac ctcgcgcgcg tcgtcgtcga aggcggcgca
     6061 gatgtggcgg aaggcttcgt ccgggtcgag gcgcgagagg tctgcgagcg cggcggcgcg
     6121 gtctcctgtg tcggcgctcg tgatgcgctg ctggatcgcc cgcggcaccg tcgaaagctc
     6181 gtcgaactgc tcgatgccct tctcttccgc ggcgcgcggc ggcggcgcct cgccgcgtcc
     6241 gaaggctcct gcttgcggga cgtcccgtgt cgcaccggcg aacggctgtt ccggttcgcg
     6301 cgcttcgatc tcgaccggcg cggcgtcgta agccgcggcg tcagcgaact ctgcgggcgc
     6361 cgcgtccgct tcgaacgggg cgacgggcgc gaaagacgac tcctccacat caagctcgat
     6421 ccagtcctca gacgcctcgc cgggggccga cgcgggttcg agcgccttcg cctcgacttc
     6481 ggtcggcagg tcgtaactct ccgcgtccgt cgccccggcc tcttcgaccc gaacgggttg
     6541 cgcggctctc tcatcgacga acggcgccgc ttccacgacc ggcgactccg tgaccggggc
     6601 tgcctcgccc cgcgtctcct cgaccgccgc cgcgtggccc gcggccacca gctcgaacca
     6661 cgcggcggcg tcttcgatgg acggctcgac ctctttcggc tcgaccctga cgcccgcgtc
     6721 gccctcgggc gaaatgacga tctccgtctc cgccgaagcc gccgcctccg actgctcggc
     6781 ggcgcgctcc tcctcgacgg cgctcgcttc cgccgcgaaa ctctcttcgc cgcgctgccc
     6841 ttcgacgttc acgtcgggcg cctgtccgac cgaagcggtc tcggcggcgg gcgcgacgaa
     6901 gggaggcgcg gcgtcggcgg tgccttccct gcaaatctct atggcgtttt gcagggtgcg
     6961 gcgcgggagc ttttcgcgtt gtttcttctg ccgctcgcgc aggtcttcga gcgggccgac
     7021 ggcggcgggg tcacgaaggt ctgcgagcgc ctcgaccgcc gcgcggcgca cgtcgatggc
     7081 gttgtcttcg agcgcggcga cgaggtgcga agttgccgcg cggtcgccgg cgagcgccag
     7141 cgagcgcgcg gcggcggcgc gttcggcggg cgcctccgcg tagtgcaggt cgtgcgccgc
     7201 ctcgttgaag aagccgtgct cgatgaaggc cgcgcccgcg cgcccgcggc gctcggcgtt
     7261 gcgccccgcg agcgcggaga gcagttcggc ggcgatcatc tgtcgcccca tcatgtcggc
     7321 tgacctgagc acgccgcggt cgtagcgctc gccgtccagc agccgcttcg tctccgcctg
     7381 cacgctctcc gcgtccgccg cgacggtcga gtcggtctcg gcgtccggcg aagcaacagc
     7441 ctccgccgac gcgacggcct cgacagccgt cacgacctcg cccgcctgcg cggtgccgac
     7501 ctcggcggtc tccacggcca catcttccgc gacatccccg acgggaagat cggcgaccgc
     7561 gtccgcggcc tccgcctctt cggcgaaccg cgcctcggtc gagtgctcga cgacgggcgg
     7621 ctcggcgtca gccgcttctt caacgaccgt ctccccaacc gcccccgcct cgccgacggt
     7681 cgtctcgata gcagcatctt cgaccgtcgc cgcttcgacc gtcgcttctt cgacgactac
     7741 atcttcggca gcggcttctt cgacaacggc ctcttcgacc gccgcttctt cagccgcgga
     7801 ctcctcagca acgacctctt cgacgggcgc ctcttcgaga acaacatctt cggcgacggc
     7861 ttcttgagct gccgccgctt cttcttcgac gaatgtctct tcgaccgcgc gcggctcggg
     7921 ttcggcggtc agcggttccg ccttcgtcgt ctcgtcaacg gtctcttcat ccgtcgccgc
     7981 cgcgcccgcc agtgccgagg cggcgacgag cggcgcaagc gtgagggatt cgacgacagg
     8041 cgtcgtggcg ggcatgtccg ccgacacgag cgccggagtc tcttcctgta cgacggcggg
     8101 ggcgggaggt gcttccctga gtttcgcctt gcccgccttc ttatgcttct tcctgtccgt
     8161 ctgctgcctg ccgggcttcg ccttgctctc ttcttcagcc ttcacgaccg gcactaaagg
     8221 ctttgccggt tcgaccgcta gcggcttcgc ttctgtggta gctgccggtt tcgcttcttc
     8281 gatggctgcc ggtttcgcct ctgcgacggg agcgggtttg gcttcttcgg gtgcgtgcgg
     8341 cgcgcggctc gtcgcggagg agcggcgcgc ggcgaagaag aggccgaggc cgacgaccag
     8401 cagggcgccg atgagcgcga gcggccaatt gcgcagcagc gtcgcgccga acgaagggcc
     8461 gggcactgcc gtcgtcgcgg gcgcgttcgt gcgcgtcgcg ggcgcgttcg tttcg
//
LOCUS       DBNGOH010001006         5702 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4001394, whole genome shotgun sequence.
ACCESSION   DBNGOH010001006 DBNGOH010000000
VERSION     DBNGOH010001006.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5702)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5702)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5702
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4001394"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1696
                     /locus_tag="AC159P_19585"
     CDS             <1..1696
                     /locus_tag="AC159P_19585"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3697698.1"
                     /translation="ESNEETTAFVVGLHEQQVGKIRTALWVLLGAVALVLLIACSNVA
                     NLLLARAASRQKELAIRAALGAGRRRIVRQLLTESLLLSLLAGAAGILLAWWGVDALV
                     ALGPADLPRLDGVRLDASVFGFALLLSALTGLVFGLVPALKASRPELEETLREGGGRG
                     STAGRNRQRLRGLLIVSETALALVLLVCAGLLVKSFVHLLRVDPGFDPENVLTLGVSL
                     PYAKYEEAAPRDAFVSQSLERVRAVAGVETAAFVSPLPFSNANLVGDFQIVGRPAPLT
                     ATETPSAAVRTVTPDYFRVMGIPLRRGRPFTEQDRKGAPGAAIVNETLARRYWPGQDP
                     IGQRIKGLGVNLTGDEPPDWEIVGVIGDVRHSGLDSEPDGELYVPYHQNTWSWGNFVV
                     RTAVEPSGLASAVRREVMTSDKEQPVVNVKPLAALIAESVAQRRFYMLLLGGFSAVGL
                     CLALVGIFGVVSYTVTERTHEIGLRLALGASPGAVVRLMIRGGMISALAGIALGVAAA
                     LVAARYMSTLLFDVTATDLFVFACVPLLVALVALAACYLPARRATKVDPMVALRYE"
     gene            1726..4158
                     /locus_tag="AC159P_19590"
     CDS             1726..4158
                     /locus_tag="AC159P_19590"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3697699.1"
                     /translation="MGKLWQDVRYSFRLLWKDKGFAAIAVLTLALGIGANTAIFSVVD
                     ATLLRPLPYRDPDRLVHLWETSPQQQQFAEREASYPDFLDWKEQNRVFEGVGGYSRRS
                     FTLTGRDAPDLLRGVAVTDGFFQVLGVEPVAGRSFQAGEDRPGAEPLVLLGHDTWRRR
                     FNTDPQVVGQTLTLNGNSHRVVGILPASFQFAPAGEADVWVPLNPNEQQRTRRFMHWL
                     NVVGRLKPGVSVEQAKADMDTIARRIAGAYPDSHTGTSVRVVGLHEQIVGKLKPILYV
                     LLGTVCFVLLIACANVANLLLARSAARQKEIAIRAALGAGRGRLLRQLLTESTVLALA
                     GGAAGALLASWGVDLLVARLPEAQLSSMPYLRGLSSNPNVLAFTSVISLLTGVVFGLA
                     PALQASKLNLQDSLKEGGRTSSAATHQRLRNLFVVSEIALALLLLVGAGLVLKSLSRL
                     LEVRPGFNPDNVLTMRVPLPPDKYPEDGHLVSFHGQLLGRLETLPGVKGVATVSVIPL
                     TGGNTSRFIAEGRPAPPPGSELNANIRDITPNYFEVMDVPLLRGRPFSEQDKADSPAV
                     VIVNQTFADRLFPGEDAVGKRLLLPSVQRPPIEIVGVVADEKVTALDAATTPVVYGPF
                     QQDPDRTLSVVVRTSADPGGVFGAVRGEIRSLDPNLPVYDIRTMQQVIDRSPSIFLRR
                     YPAFLISTFAAVALVLAVIGIYGVISYTVTQRTHEIGLRMALGAQSGDVLRMVLWQGL
                     KLILLGLAAGLAAAFALTRLMSNLLFGVSATDPLTFTGISLLLTAVAALACYLPARRA
                     TKVDPMVALRYE"
     gene            4289..>5702
                     /locus_tag="AC159P_19595"
     CDS             4289..>5702
                     /locus_tag="AC159P_19595"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3697700.1"
                     /translation="MGTLWQDLRFGFRLLLGSPGFTAVAVLALALGVAANTAIFSVVN
                     TVLLQPLPYKDAARLAMVWEHNRIRDKRQNVVSPANFIDWKEQSTVFEDIAAFGDFRA
                     NLTGGDEPVEVSLQYNTPNLFSVLGAVPILGRDFTADDAKPDAARVVMLSYGLWQRRF
                     GGDPKIVGKTVTVNASPAEVIGVRPADFQWFVRKSTFAGRPPEMWTPFGFTEQHRRRG
                     GRYMSAVARLKPGVGPAQAQTELSHIAARLEQQYPDFNTGWGVEVVPLREQFSGQIST
                     ALWILLGAVGFLLLIACANVANLLLARSAARRKEIAIRTALGAGRLRVVRQLLTESFL
                     LALAGGALGLLLAWWGVELLVALSPSDLLDIGGVKLNLPVLGFTLGVSLLTAIVFGMA
                     PAIEASRADTSESLKEGARGTTGGPRSRRMRAAFVVTEIALALLLLVGAGLTAKSFVR
                     LQSVGPGFDPEGVLTMRVLLP"
BASE COUNT          818 a         1968 c         1921 g          995 t
ORIGIN      
        1 ggagtcgaac gaggagacga cggccttcgt cgtcgggctg cacgagcagc aggtggggaa
       61 gattcgcacg gcgttgtggg tgctgctcgg cgcggtcgcg ctcgttctct tgatcgcctg
      121 ctcgaacgtc gccaacctgc tgctggcgcg cgccgcctcg cggcagaaag agctggcgat
      181 ccgcgcggcc ctcggcgcgg gccgccggcg catcgtgcgt cagctcctca cagaaagctt
      241 gctcttgtcg ctgctggcgg gcgccgccgg aatcttgctc gcctggtggg gcgttgacgc
      301 gttggtcgcg ctcggccccg cggacttgcc gcgcctcgac ggagtccgcc tcgacgcgag
      361 cgtcttcggc ttcgcgctgc tgctctcggc gctgaccggc ttggtcttcg gactcgtgcc
      421 cgcgctgaag gcttcgcgcc cggagttgga ggagacgctc agggaggggg gcgggcgcgg
      481 ctcgaccgcc ggcagaaacc gtcagcgcct gcgcggcctg ctgatcgtct ccgagacggc
      541 gcttgcgctg gtgctgctcg tctgcgccgg gttgctggtg aagagtttcg tccatctgct
      601 cagggtcgat cccggcttcg accccgagaa cgtcctgacg ctcggcgtga gcctgcctta
      661 cgccaagtac gaagaggccg cgccgcgcga cgccttcgtc agtcagtcgc tggagcgcgt
      721 gcgcgcggtg gccggcgtcg agacggcggc cttcgtcagc cccctgccct tcagcaacgc
      781 gaacctcgtc ggcgacttcc agatcgtggg gcggcccgcc ccgctcaccg ccaccgaaac
      841 gccctccgcc gcggtgcgca ccgtcacgcc cgactacttc cgcgtgatgg gcatcccctt
      901 gcgccgcggg cggccattca ccgagcagga tcgcaagggc gcgccgggcg cggccatcgt
      961 caacgagacg ctggcccgcc ggtactggcc cggccaagac cccatcggcc agcgcatcaa
     1021 ggggctgggc gtcaacctca cgggcgacga gccgccggat tgggagatcg tcggcgtcat
     1081 cggcgacgtg cggcacagcg gtttggacag cgagccggac ggagaactct acgtgccgta
     1141 ccaccagaac acctggtcgt gggggaactt cgtcgtccgc accgcggtcg agccatcggg
     1201 cctcgccagc gcggtgcgca gagaggtgat gacctcggac aaggaacagc cggtggtgaa
     1261 cgtcaagccg ctcgccgcgt tgatcgcgga gtcggtcgcg cagcggcgct tctacatgtt
     1321 gctgctcggc ggcttcagcg cggtcggctt gtgcctcgcg ctggtcggca tcttcggcgt
     1381 cgtctcctac accgtgaccg agcgcacgca cgagatcggc ctccgcctcg cgctcggcgc
     1441 aagccccggc gccgtcgtgc gactgatgat ccgtggcggc atgatctccg cgctggcggg
     1501 catcgcgctg ggagtggccg ccgccctcgt cgccgctcgc tacatgtcaa cgttgctctt
     1561 cgacgtgacg gcgacggact tgttcgtctt cgcctgcgtc ccgctgctcg tcgcgctcgt
     1621 cgccctcgcg gcctgctatc tccctgcgcg gcgggcgacg aaggtggacc cgatggtggc
     1681 gctccgctac gaatgaggga acagattcgc gggaggattc gggtaatggg gaaactctgg
     1741 caagacgtac gctacagctt ccggctgctg tggaaggaca agggcttcgc ggccatcgcc
     1801 gtcctcacgc tggcactcgg catcggcgcg aacacggcca tcttcagcgt cgtcgatgcg
     1861 acgctgctgc gcccgctccc ttaccgagac ccggatcgtc tggtgcatct ctgggaaacc
     1921 agcccgcagc agcagcagtt cgcggagcgg gaagcctcct acccggactt cctcgactgg
     1981 aaggagcaga accgcgtctt cgaaggggtg ggcggctact cgcgccgcag tttcacgctg
     2041 acgggccgcg acgcgcccga cctgctcaga ggggtcgccg tgaccgacgg cttttttcaa
     2101 gtcctcggtg tcgagcctgt ggcgggccgc agctttcagg cgggtgagga tcgacccggg
     2161 gcggaacccc tggtcttgct cggccacgac acctggaggc gccgtttcaa caccgacccg
     2221 caggtcgtcg gccagaccct gaccctcaac gggaacagcc accgcgtggt cggcattctc
     2281 cccgcgagct ttcagttcgc gcccgcgggc gaggccgacg tgtgggttcc gctcaacccc
     2341 aacgagcagc agcggacgcg ccgcttcatg cattggctga acgtcgtcgg gcggctcaag
     2401 cccggcgtct ccgtcgagca ggcgaaggcc gacatggaca ccatcgcgcg ccgcatcgcg
     2461 ggggcttatc cagactcgca cacgggcacg tcggtgcgcg tcgtggggct gcacgaacag
     2521 atcgtcggca agctcaagcc gattctgtac gttcttctgg gcacggtctg tttcgtgctc
     2581 ctgatcgcgt gcgccaacgt ggcgaatttg ttgctggcgc gttcggcggc gcggcagaaa
     2641 gagatcgcca tccgcgcggc tctgggcgcg ggccgcgggc gcctgctccg gcagctcttg
     2701 accgaaagca ccgtgctcgc gctcgcgggc ggcgcggcgg gggcgctgct ggcgtcgtgg
     2761 ggcgttgacc tgctggtggc gcgactgccg gaggcgcagc tcagctccat gccctacctg
     2821 cggggactct cttcgaatcc gaacgtgctg gccttcacgt ccgtcatctc gctcctcacg
     2881 ggcgtcgtgt tcggtctggc gccggccctc caggcctcga agctgaatct acaggactcg
     2941 ctcaaggagg gcggtcgcac ctcttccgcc gcgacgcacc aacgcctgcg aaacctgttc
     3001 gtggtttcgg agatagccct cgccctgctc ctgctcgtcg gggccggtct ggtgttgaaa
     3061 agtctctcgc gtctgctcga agtccgcccc ggcttcaacc ctgacaacgt cttgaccatg
     3121 agggtgccgc tcccccccga caagtatccc gaggacggcc accttgtctc cttccacggg
     3181 caactgctcg gtcgtttgga gaccctgccc ggcgtcaaag gcgtggccac cgtgagcgtg
     3241 attccgctga cgggcggcaa cacctcgcgc tttatcgccg aggggagacc cgcgccgccc
     3301 cccggctccg aactcaacgc gaacatacgc gacatcacgc ccaactattt cgaggtgatg
     3361 gacgtgccgc ttttgcgcgg tcgccccttt tcggagcagg acaaggcgga ttcgcccgcg
     3421 gtcgtcatcg tcaaccagac attcgccgac cgcctgttcc cgggtgaaga cgccgtcggg
     3481 aaacgcctgc tccttccgag cgtgcagagg ccgcccatcg agatcgtggg cgtcgtcgcc
     3541 gacgagaagg tgacggccct cgacgcggcg acgacgccgg tcgtctacgg ccccttccag
     3601 caagaccccg accgtacgct cagcgtcgtc gtgcgcacct cggctgaccc cggcggcgtc
     3661 ttcggcgccg tccgcggcga gatacggagt ctcgacccga accttccggt ctacgacatc
     3721 cgcaccatgc agcaggtgat cgaccgttcg ccttcgatct ttctgcgccg ctacccggcg
     3781 ttcctcatca gcactttcgc cgccgtggcg ctcgtgctgg cggtcatcgg catctacggc
     3841 gtcatctcct acacggtgac gcaacgcacg cacgagatcg gcctgcggat ggcgctcggc
     3901 gcgcagtccg gggatgtgct caggatggtg ttgtggcagg gcttgaagct catcctgttg
     3961 ggactggcgg cgggactggc ggcggccttc gccctgacgc ggctgatgtc gaacctgctg
     4021 ttcggcgtga gcgcgaccga ccccttgacc ttcacgggca tctcgctgct gctcacggcg
     4081 gtcgccgcgc tggcctgtta tctcccggcg cgccgcgcga cgaaggtaga cccgatggtc
     4141 gcgctccggt acgaataaag ccgtaagccg tgaaccggaa accgtgacca gtaaaagacc
     4201 gtgagccgtg aaccgtgacg agtccgaacc ggttttcacc tgtcacggtt cacggttcac
     4261 ggcttacggc ttttggaggt gtttgaagat gggcacactc tggcaagacc tgcgcttcgg
     4321 cttccgccta ctgcttggaa gtccgggctt tactgccgtg gccgtgctcg cgctcgccct
     4381 cggggtggcg gccaacacgg cgatcttcag cgtcgtcaac acggtgctct tgcagccgtt
     4441 gccctacaaa gacgcggcgc ggctcgcgat ggtctgggag cacaaccgca tccgcgacaa
     4501 acgccagaac gtcgtctcgc cggcgaactt catcgactgg aaggagcaga gcacggtctt
     4561 cgaggacatc gcggccttcg gagactttcg cgccaacctg acgggcggcg atgagccggt
     4621 cgaagtctcg ctccaataca acacgccgaa cctcttctcg gtgctcggcg ccgtgccgat
     4681 actggggcgc gacttcaccg cggacgacgc gaagcccgac gcggcccgcg tcgtcatgct
     4741 gagctacggc ctctggcagc gacgcttcgg cggcgacccg aagatcgtcg gcaagaccgt
     4801 cacggtcaac gcctcgccgg cggaggtcat cggcgtgagg cccgcggact tccagtggtt
     4861 cgtcaggaag agcacgtttg cgggcaggcc gcccgagatg tggacgcctt tcggcttcac
     4921 tgagcagcac cggcgccgcg ggggtcgtta catgtcggcg gtggcgcgcc tcaagccggg
     4981 cgtcggcccg gcccaggcgc agaccgagtt gagccacatc gccgcgcgcc tcgaacagca
     5041 gtacccggac ttcaatacgg ggtggggcgt ggaagtcgtg ccgctgcgcg aacagttctc
     5101 cgggcagatc agcaccgcgc tgtggatact gctcggcgcg gtcggcttcc tgttgctcat
     5161 cgcctgcgcc aacgtcgcca acctgctgct cgcacgctcg gcggcgcgcc gcaaagagat
     5221 cgccatccgc acggcgctcg gcgcgggccg cctgcgcgtc gtgcgtcagc tcttgacgga
     5281 gagcttcctg ctcgcactcg cgggcggggc gctcggactc ctgctcgcct ggtggggcgt
     5341 cgaacttctc gtcgcactga gtccgagcga cctgctcgac atcggcggcg tcaagctcaa
     5401 cctgccggtg ctcggcttca cgctcggcgt gtcgctgctg accgccatcg tcttcggcat
     5461 ggcgcccgcg atcgaagcct cgcgcgccga cacgagcgag tcgttgaagg agggcgcgag
     5521 gggcacgacc ggcggcccgc gcagccgacg catgcgcgcg gcgttcgtcg tcaccgagat
     5581 cgcgctcgcg ctgcttctgc tggtgggtgc gggcctgacg gcgaagagct tcgtgcgtct
     5641 acagtccgtc ggccccggct tcgacccgga gggagtgctg acgatgcgcg tgttgctgcc
     5701 tg
//
LOCUS       DBNGOH010001007         9064 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3633230, whole genome shotgun sequence.
ACCESSION   DBNGOH010001007 DBNGOH010000000
VERSION     DBNGOH010001007.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 9064)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9064)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9064
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3633230"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..348
                     /locus_tag="AC159P_19600"
     CDS             <1..348
                     /locus_tag="AC159P_19600"
                     /inference="COORDINATES: protein motif:HMM:NF020133.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HIR3697701.1"
                     /translation="AVYNLQRAALFGAALEAGDDELVWEAMRDRLHQPHRRGLVPGLA
                     AALDTPRQKGLVGLALSGAGPSVVALARGRFEEIGEAVAEGFRRAGVAATARVLEVDE
                     EGLSSRTASAGMN"
     gene            380..1642
                     /locus_tag="AC159P_19605"
     CDS             380..1642
                     /locus_tag="AC159P_19605"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HIR3697702.1"
                     /translation="MADGVSLVEEVSARWNGAEKFPLASRVAGMSMSKTMAVSALAEQ
                     LRRKGADVLDLGAGEPDFPTPENVRRAAYAAMEGGHTKYTPAAGTLRLKQAICDRFAA
                     DFGAAYEPAEVIAGAGAKQVIFNAVATLIEPGDEVLVPKPYWVTFPEAVTFAGGQSVW
                     VETESEGFRLTAAAVERAITPRTKLLIVNSPSNPSGLVVAPEEFERIVAVAARRGVWV
                     ISDECYSQFVYEPARPFSAAQLPEELRRHVLVAGSLSKTYAMTGWRIGYALGPREWVA
                     AMTCVQSHSTSNPTSVSQEAAVEALTGPQDSVAEMLAAYRERRDWLIPALNELPGVTC
                     AMPDGAFYAFPCVKGALGQNYIQTSEELTTFLLEEAHVAVTPGDAFGAPGYLRLSYAT
                     SLDVLRRAVERIADALAKCERESNCWAV"
     gene            1765..2712
                     /locus_tag="AC159P_19610"
     CDS             1765..2712
                     /locus_tag="AC159P_19610"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 -
                     branched-chain-amino-acid:2-oxoglutarate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HIR3697703.1"
                     /translation="MTFDATKYVWMNGGCVRWDEATVHVSAHALHYGSGVFEGMRCYE
                     TEDGPAVFRLGEHLDRLYASAAVYGMEIPFAREELAEAVSEVIGLNGFRSCYVRPICF
                     YGSRTLGVHPRQCPVETVILAWPWAAYLGDEGLDAGVRVTVSPWLKFHTRMMPTTAKA
                     CGQYLNSILAVRDAASRGFAEALLLDAEGRLAEGSGENLFVVRGGRLLTNDERHSILL
                     GITRDAVIRIARDLGYEVETRALELCDLEAADEAFFTGTAVEVTPIREVDGALVGGGQ
                     RGPVTERIQRAFFDATAGRDPRYRHWLSHVAPQPVASGV"
     gene            complement(2870..3961)
                     /locus_tag="AC159P_19615"
     CDS             complement(2870..3961)
                     /locus_tag="AC159P_19615"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013927870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HIR3697704.1"
                     /translation="MDESAKSSDGPRAVRAGEELDTEKLLAYLREHMPETEGPLEVEQ
                     FPAGFSNLTYLVRAGGREFVLRRPPHGSKVKTAHDMGREFLILSRLRPVYAKVPRPLL
                     YCEDASVLGAPFYLMERVRGRILRARPPAGLELTPEVMRRLSELLVGNLAEIHGVDHV
                     AAGLGEIGRPEGYVARQVEGWTKRYFAARTDEVPSVERLARWLAEHAPRDSGGALIHN
                     DYKFDNVVFAPEDLSRVVAVLDWEMATIGDPLMDLGTTLGYWTDPDDPEEWRRLGFGL
                     TTLPGSLRRREVLERYARQTGRDVGDAVFYFAYGLLKIAGIVQQIYYRYRQGLTKDER
                     FARLGLLVRACGEMAGRAIEKGRVDELSK"
     gene            complement(3975..5180)
                     /locus_tag="AC159P_19620"
     CDS             complement(3975..5180)
                     /locus_tag="AC159P_19620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009184052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR3697705.1"
                     /translation="MNIPEGKLASLLSDIREFIREEALSLEAEFLRGRFRDLLPTLAE
                     KRGRVKELGLWAPQLPRELGGLGLKLTEFAHVSEELGRTPLGHYLFNCQAPDAGNLEV
                     LHAHATPAQREAYLLPLARGEVRSCFSMTEPEHPGSNPVWMSTTAVREGGEYVINGHK
                     WFTSAADGAAFAIVMAVTAPEAESLHKRASQIIVPTDTKGFRIVRTVSVMGHEGSDYA
                     SHAEVVYEDCRVPVSNLLGQEGEGFRIAQERLGPGRIHHCMRWVGVCERAFEMMCERA
                     ARRELRPGVALATRQSVQEWIAESRAEISAARLLVLHTAERLDREGPHAAREDISLIK
                     FYVAGVMHRVLDRAVQVHGALGLTDDTVLAYWYRHERAARIYDGPDEVHKSVVARSVL
                     RRYGVEIAI"
     gene            5366..6022
                     /locus_tag="AC159P_19625"
     CDS             5366..6022
                     /locus_tag="AC159P_19625"
                     /inference="COORDINATES: protein motif:HMM:NF012655.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR3697706.1"
                     /translation="MPKLSREVLEEKRGRIEDAAKELFIKRGFHATSMRDVASGAGTS
                     LGNLYNYYKTKEDILESIIARYQKVIDARLRTIFDEIEEPFDPDSLARFGRMIKELVS
                     THSDFWLLMYIDVLEFENRHFRKMFDGLAKNLRRRFAPYFERLREEDALYDGIDPAVG
                     FTAAYMQFFNYFLVEKLFGGNRHLGMSDDRVIATLTDIFVRGVMKPEKQKTAHAVRAK
                     "
     gene            6227..>9064
                     /locus_tag="AC159P_19630"
     CDS             6227..>9064
                     /locus_tag="AC159P_19630"
                     /inference="COORDINATES: protein motif:HMM:NF051698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR3697707.1"
                     /translation="MFSVKRLASVALLASALALGALAQGATGQLTGTVSDPQGGRVAG
                     ASVVVTSLDTGAARAVETDGDGNFAAALLPPGRYKVEVSAQGFGRILVESAIVNVTQT
                     TTLSDLVLSPALQGETVTVTDQGQGSLVQQESSQVGRVIQGETLRQLPLPTRNFQQLL
                     TLSPGTSASVSNNTELGRGDSIISVNGQRTTSNNVRINGIDANSVGTNSTPNIAVPAT
                     DSLQEFIVQTSLYDASQGRNAGGNVEAVTRSGGSEFHGNVYEFFRNSRLNANDFFLNA
                     AGRPRPVLNRNQFGGTLGGPIIAQKFFFFGSYQGTRERNGASLNNSLTFPVLPAGLRD
                     DNRTAAGLAATFGLPAASISPITVALLNARLPSGELAIPSPNASGLAPLAGVLTPRSG
                     VSTFRENQFNANFDYTFGEDHTLSVKNFFASNPTFQSNYNFAGLGNGPLQLPGTGGDL
                     DIIQALSSISDTYVFSPDVINQARFGFSRLRVTSVPEEPFTASEFGITNPLANQFPGL
                     PTITVTGLFNIGSSPLADQSSRINTFTFADTLSVVAGAHRLKIGGEYRRSQVNFYFNA
                     FTRGQLIFPTFANFLLGQSVSIIGSGVYDRAFRTNDLSGFVQDDWKASDRLTLNLGLR
                     YDFYGLPKEKYGRLVNFDPAQFRNGTLASPAAPPNGFVQAGNAVNPLPGVPLVSDTLV
                     PQDKNNFSPRVGFAYRVNEEGTLAVRGGYGVYFDRVSTRFANTQLFNFPYLALGVGIF
                     RPLSTPFAPVPQPGTFPVGATIPSPLAPLGILISGVYVNPTLRTPYIQQYNANVQWEF
                     AKDYLLEVGYIGSKGTKLLQLLTLNQPIYNPATNTFAAPFAPFISAQKNATGGLQAVS
                     TDSNSNYNSLQVSVTKRFSRGLQFLAAYTYGKSIDYYSGGPVNELVSTPGDQRDWKSN
                     RGLSDFDRRHRFSVSYSYAIPAFSTSRFVQGW"
BASE COUNT         1491 a         3248 c         2851 g         1474 t
ORIGIN      
        1 gccgtctaca atctacagcg cgcggcgctg ttcggcgcgg cgctcgaagc gggcgacgac
       61 gaactcgtgt gggaggcgat gcgggatcgc ctgcatcagc cgcatcgtcg cgggctggtg
      121 ccgggactcg ccgcggcgct cgacacgccg cgacagaagg ggctggtcgg cctcgcgctg
      181 agcggggcgg ggccgagcgt ggtggcgctt gcgcgcggac gctttgaaga gatcggcgag
      241 gcggtggcgg aaggcttccg ccgtgcgggc gttgcggcga cggcgcgcgt gctcgaagtt
      301 gacgaggaag ggttgagtag tcgaacagct tccgcaggga tgaactaaca aagggatgaa
      361 caaacaagag gagtgaatga tggctgatgg cgtttcgttg gttgaagagg tgagcgcgcg
      421 ctggaacggc gcggagaagt ttccgttggc gtcgcgcgtg gcggggatgt cgatgtcgaa
      481 gacgatggcg gtgtctgcgc tggccgagca gctcaggcgc aagggcgcgg acgtgctcga
      541 cctcggcgcg ggcgagccgg acttcccgac gcccgagaac gtgaggcgcg cggcctacgc
      601 ggcgatggag ggcgggcaca cgaagtacac gcccgccgcc gggacgctgc ggctgaagca
      661 ggcgatctgc gaccgcttcg ccgcggactt cggcgccgct tacgagccgg cggaagtcat
      721 cgcgggcgcg ggcgccaagc aggtcatctt caacgcggtc gcaaccttga tcgaaccggg
      781 cgacgaagtg ctggtgccga agccgtactg ggtgacgttc cccgaggccg tcacgttcgc
      841 gggcggccaa agcgtgtggg tcgagaccga aagcgaaggc ttccgcctca ccgccgcggc
      901 ggtcgagcgc gccatcacgc cgcgcacgaa gctgctgatc gtcaactcgc cttcgaaccc
      961 ctcgggcctc gtcgtcgcgc ccgaagagtt cgagcgcatc gtcgcggtcg ccgcgcgccg
     1021 cggcgtgtgg gtcatctccg acgagtgcta cagccagttc gtttacgagc cggcgcggcc
     1081 cttctcggcg gcgcagttgc cggaggagtt gcgccgccac gtactggtcg cggggtctct
     1141 gtcgaagacc tacgcgatga cgggctggcg catcggctac gcgctcggcc cgcgcgagtg
     1201 ggtcgcggcg atgacgtgcg tgcagagcca ttcgacctcg aacccgacct cggtttcgca
     1261 ggaggcggcg gtcgaggctt tgaccggccc gcaggactcg gtcgcagaga tgctcgccgc
     1321 ctaccgcgag cggcgcgact ggctcatccc cgcgctgaac gaattgcccg gcgtcacctg
     1381 cgcgatgccc gacggcgcgt tctacgcctt cccctgcgtg aagggcgcgc tcggccagaa
     1441 ctacatccag acctcggaag agctgacgac attcctcctc gaagaggcgc atgttgccgt
     1501 cacgccgggc gacgccttcg gcgcgccggg ctacctgcgg ctctcctacg ccacctcgct
     1561 cgacgtgctg cgccgcgccg tcgagcgcat cgccgacgcg ctggcgaaat gcgaacgcga
     1621 atcgaactgc tgggcggtgt gaaaagcaat gatgaatgcg gaatgatgaa tgatgaatca
     1681 aaggcacgct gcctttcatc attcaactcg tcttccgatt catcattcat cattccacat
     1741 tcatcatttc cgttaggtgt gaagatgact ttcgacgcga ccaagtatgt gtggatgaac
     1801 ggcggctgcg tgcgttggga cgaggcgacg gtgcacgtct cggcgcacgc gctgcattac
     1861 gggagcggcg tgttcgaagg gatgcgctgt tacgagacgg aggacggccc ggcagttttc
     1921 cgcctcggcg aacacctcga caggctctac gcctcggcgg cggtctacgg gatggagatt
     1981 cctttcgcgc gcgaagaact cgcggaggcc gtgtccgaag tcatcggcct caacggcttc
     2041 cgcagttgct acgtgcgccc gatctgcttc tacggcagcc gcacgctcgg cgtccacccg
     2101 cgccagtgcc cggtcgagac ggtcatcctc gcgtggccgt gggcggccta cctcggcgac
     2161 gaggggctgg acgcgggcgt gcgcgtcacc gtctcgccgt ggctcaagtt ccacacgcgg
     2221 atgatgccga ccaccgcgaa ggcgtgcggc caatacctga actcgatcct cgccgtgcgc
     2281 gacgccgcga gccgcggctt cgccgaagcg ctgctcttag acgcagaggg acggctcgcc
     2341 gaaggctcgg gcgagaacct cttcgtcgtc cgcggcgggc ggctgctgac gaacgacgag
     2401 cgccactcca tcctgctcgg catcacgcgc gacgccgtca tccgcatcgc gcgcgacctc
     2461 ggctacgaag tcgagacgcg cgcgctcgaa ctttgcgacc tcgaagccgc ggacgaagcc
     2521 ttcttcaccg gcaccgccgt cgaagtcact cccatccgcg aagtggacgg cgccctcgtc
     2581 ggcggcggcc agcgcggccc cgtcacggag cggattcagc gcgccttctt cgacgccacc
     2641 gccgggcgcg acccgcgcta ccggcactgg ctcagccacg tcgccccgca gccggtcgcc
     2701 agcggagtct gacgcaagta ggcggtaggc agtaggcagt aggcagtcag ggcaagaggc
     2761 ctgggaggta aagtcccaag cctctttgtt cgtcaagtaa tccgagattg aggctcggga
     2821 ctttatctcc caggactctt cgcccttctt gcctcttact gcctatcgcc tacttgctca
     2881 gctcgtccac gcgccccttc tcgatggcgc gcccggccat ctcgccgcag gcgcgcacca
     2941 gcaggccgag ccgcgcgaag cgctcgtcct tcgtcaagcc ctgccggtag cggtagtaga
     3001 tttgctgcac gatgcccgcg atcttcagca gcccgtaggc gaagtagaag accgcgtcgc
     3061 ccacgtcgcg ccccgtctgc cgcgcgtaac gttccagcac ctcgcggcgg cgcaggctcc
     3121 ccggcagcgt cgtcagcccg aaccccagac gccgccactc ctccgggtcg tccgggtcag
     3181 tccagtagcc gagcgtcgtc cccaagtcca tcagggggtc gccgattgtc gccatctccc
     3241 agtcgagcac ggcgaccacg cgcgacaaat cttccggcgc gaagaccacg ttgtcgaact
     3301 tgtagtcgtt gtgaatcagc gcgccgcccg aatcgcgcgg cgcgtgctcg gccagccagc
     3361 gcgcgagcct ctccaccgaa ggcacttcgt ccgtgcgcgc ggcgaagtaa cgcttcgtcc
     3421 acccctcgac ctgccgcgcg acgtagcctt cgggccgacc gatctcgccg aggccggcag
     3481 caacatgatc gaccccgtga atctccgcga ggttcccgac gaggagttcg gacaggcgcc
     3541 gcatcacctc cggcgtcaac tccaaccccg cgggcggtcg cgcgcgcagg atacgtccgc
     3601 ggacgcgctc catcaggtag aacggcgcgc cgagcaccga cgcgtcctcg cagtagagca
     3661 aagggcgcgg caccttcgcg tagacaggcc gcagccgcga caggatcagg aactcgcgcc
     3721 ccatgtcgtg cgccgtcttc accttcgacc cgtgcggcgg tcggcgcaac acgaactcgc
     3781 gcccacccgc gcgcaccaga taggtcaggt tcgagaagcc cgccgggaac tgctcgactt
     3841 cgagcgggcc ttccgtctca ggcatgtgct cgcggagata agcgagcagc ttctccgtgt
     3901 ccaactcctc gcccgcgcgc acggcccgcg gcccgtcgct cgacttcgcg ctctcgtcca
     3961 aagcgaatga cctctcagat cgcaatctcg acgccgtacc ggcgcagcac gctccgcgcc
     4021 acgaccgact tgtgcacctc gtccggcccg tcgtagatgc gcgccgcccg ctcgtgcctg
     4081 taccagtagg cgagcaccgt gtcgtccgtg aggcccagcg cgccgtgcac ctgcaccgcg
     4141 cggtcgagca ccctgtgcat cacgcccgcc acgtagaact tgatgagcga gatgtcctcg
     4201 cgcgcggcgt gcggcccttc gcggtcgagc cgctcggcgg tgtgcaggac gagcagccgc
     4261 gcggcgctga tctcggcgcg gctctcggcg atccactcct gcacgctctg cctcgtcgcc
     4321 agcgcgacgc cgggccgcag ctccctgcgc gcggcgcgtt cgcacatcat ctcgaacgcg
     4381 cgctcgcaca cgccgaccca gcgcatgcag tgatgtatgc gccccggccc caaacgctcc
     4441 tgcgcgatgc ggaagccctc gccctcttgg cccaacaaat tactcacggg cacgcggcag
     4501 tcttcgtaca cgacctcggc gtggctcgcg tagtcgctcc cctcgtgccc catcacgctc
     4561 acggtgcgga cgatgcggaa gcccttcgtg tccgtcggca cgatgatctg gctcgccctc
     4621 ttgtgcaggc tctccgcctc gggcgcggtg acggccatga cgatggcgaa cgccgcgccg
     4681 tccgccgcgg acgtgaacca cttgtggccg ttgatgacat actcgccgcc ctcgcgcacg
     4741 gcggtcgtgc tcatccacac ggggttcgag ccggggtgct ccggctccgt catcgagaag
     4801 cagctccgca cctcgccgcg cgcgagcggg agcaggtaag cctccctctg cgcgggcgtc
     4861 gcgtgcgcat gcagcacttc gaggttgccc gcgtccggcg cctggcagtt gaagaggtag
     4921 tggccgagcg gcgtgcgccc caactcctcg ctcacgtgcg cgaactccgt cagcttgagg
     4981 ccgaggccgc ccagctcgcg cggcaactgc ggcgcccaca gccccagctc cttcacgcgc
     5041 ccccgcttct ccgcgagcgt cggcagaaga tcgcggaagc gcccgcgcaa aaactccgct
     5101 tcgagcgaca gcgcctcttc gcggatgaac tctcggatgt ccgagagcag ggaagcgagc
     5161 ttgccttcag ggatgttcat aaagtttcgc gcgtctcggt tcagcttcgt ctcgggcttc
     5221 tcgcccgcct cattaacaca ccggcggcgg gcgtgtccaa cattttctcc cgcgggccgc
     5281 aggcgcgggg cctccgcccc gccctcgaat aagtggttga caattcgccg ccgctcggat
     5341 aaaaaggcgg acgttcgttt tttttatgcc gaagctgagc cgcgaagttc tggaagagaa
     5401 gcggggccgc atcgaggacg cggccaaaga gctgttcatc aagcgcggct tccacgccac
     5461 ctcgatgcgc gacgtggcct ccggcgccgg cacctcgctc ggcaacctgt acaactacta
     5521 caagaccaag gaagacatcc tcgaatcgat catcgcgcgc taccagaagg tgatcgacgc
     5581 gcgcctgcgc acgatcttcg acgagatcga ggagccgttc gaccccgaca gcctcgcccg
     5641 cttcgggcgg atgatcaagg agctggtgag cacgcactcg gacttctggc tgttgatgta
     5701 catcgacgta ctggagttcg agaaccgcca cttccgcaag atgttcgacg ggctggcgaa
     5761 gaacctgcgc cgccgcttcg ccccctactt cgagcggctg cgcgaagagg acgcgcttta
     5821 cgacggcatc gacccggcgg tcggcttcac ggcggcctac atgcagttct tcaactactt
     5881 cctcgtcgag aagctgttcg gcggcaaccg ccacctcggc atgagcgacg accgcgtcat
     5941 cgcgaccctc acggacatct tcgtccgcgg cgtgatgaag cccgagaagc agaagacggc
     6001 gcacgccgtc cgcgcgaaat aatcacggcc cgccgtccgc accgcccctg acaggcgcgc
     6061 gggcgcggcg ggcgaacatt ccgcagacag aggccggccc ggcccggcgc ccgcaagtgg
     6121 agacgccgca cggccttccg ccggggaaag aaagacggac gttcgttttt atttgttccg
     6181 cggtcgggca aaacccctta gtttccactg taggaggtag aaagagatgt tttcagtgaa
     6241 acgcttagct tcggtcgcgc tgctcgcgtc ggcgttggcg ttgggcgcgc tcgcgcaggg
     6301 cgccacgggg cagttgacgg gcacggtctc cgacccgcag ggcgggcgcg tggcgggcgc
     6361 gagcgtggtc gtcaccagcc tcgacacggg ggccgcgcgc gcggtcgaga cggacggcga
     6421 cgggaacttc gcggcggcgc tgctgccgcc gggccgctac aaggtcgagg tctcggcgca
     6481 ggggttcggc agaatcctcg tcgagagcgc catcgtcaac gtgacgcaga cgacgacgct
     6541 ctccgacctc gtgctctcgc ccgcgcttca gggcgagacc gtgacggtga cggatcaggg
     6601 gcaaggctcg ctcgtgcagc aggagagttc gcaggtcggg cgcgtcatcc agggcgagac
     6661 gctgcgccaa ctcccgctcc cgacgcgcaa cttccagcag cttttgacgc tctcgcccgg
     6721 cacctccgcg agcgtctcga acaacacgga actcgggcgc ggcgactcca tcatcagcgt
     6781 caacggccag cgcaccacct cgaacaacgt ccgcatcaac ggcatcgacg ccaactcggt
     6841 cggcaccaac tccaccccga acatcgccgt gccggcgacc gacagccttc aggagttcat
     6901 cgtccagacc tcgctctacg acgcctcgca ggggcgcaac gcgggcggca acgtcgaggc
     6961 cgtgacgcgc tcgggcggct ccgagttcca cggcaacgtc tacgagttct tccgcaacag
     7021 ccgtctgaac gccaacgact tcttcctcaa cgcggcgggc cggccccgcc ccgtgctcaa
     7081 ccgcaaccag ttcggcggaa ccttgggcgg ccccatcatc gcgcagaagt tcttcttctt
     7141 cggctcgtat caggggacgc gcgagcgcaa cggcgcgtcg ctcaacaaca gcctgacttt
     7201 tccagtcctc ccggctggcc tgcgcgacga caaccgcacg gcagcagggt tagccgcgac
     7261 gttcggcctg cctgccgcca gcatcagccc catcacggtc gcgctgctga acgcgcgcct
     7321 gccttcgggc gagctggcca tcccctcgcc gaacgcgtcg ggcctcgcgc cactggcggg
     7381 cgtgctgacg ccgcgctccg gcgtctcgac cttccgcgag aaccagttca acgccaactt
     7441 cgactacacg ttcggcgaag accacacgct ctccgtgaag aacttcttcg cctcgaaccc
     7501 gaccttccag tcgaactaca acttcgccgg attggggaac ggcccgctgc aactgccggg
     7561 cacgggcggc gacctcgaca tcattcaggc gctctcgtcc atctcggaca cctacgtctt
     7621 cagccccgac gtgattaatc aggcgcgctt cggcttcagc cgcctgcgcg tcacgtccgt
     7681 ccccgaagag cctttcacgg cgtccgagtt cggcatcacg aacccgctgg cgaaccagtt
     7741 cccgggcctg ccgacgatca ccgtcacggg cctcttcaac atcggctcgt cgccgctggc
     7801 cgaccagtcc tcgcgcatca acacgttcac cttcgccgac acgctctccg tcgtcgccgg
     7861 ggcacaccgg ctgaagatcg gcggcgagta ccgccgctcg caggtcaact tctacttcaa
     7921 cgccttcacg cgcggccaac tgatcttccc gaccttcgcc aacttcctgc tcggccagtc
     7981 ggtctcgatc atcggctccg gcgtctacga ccgcgccttc cgcacgaacg acctctcggg
     8041 cttcgtccag gacgattgga aggcgagcga ccgcctgacg ctcaacctcg gcctgcgcta
     8101 cgacttctac ggcctgccca aagagaagta cgggcggctc gtcaacttcg accccgcgca
     8161 gttccgcaac ggcacgctcg cgtcgcccgc cgcgccgccc aacggcttcg tgcaggcggg
     8221 caacgccgtg aaccctctgc ccggcgtgcc gttagtctcc gacacgctcg tcccgcagga
     8281 caagaacaac tttagccctc gcgtcggttt cgcctaccgc gtcaacgaag agggcacgct
     8341 cgccgtgcgc ggcggctacg gcgtctactt cgaccgcgtc tcgacgcgct tcgccaacac
     8401 gcagctcttc aacttcccct atctcgcgct cggcgtcggc atcttccgcc cgctctcgac
     8461 gcccttcgcg cccgtgccgc agcccggcac cttccccgtg ggcgcgacca tcccttcgcc
     8521 gctcgcgccg ctcggcatcc tgatcagcgg cgtctacgtc aacccgacct tgcgcacgcc
     8581 ctacatccag cagtacaacg cgaacgtcca gtgggagttc gccaaggact acctcctgga
     8641 agtcggctac atcggctcga agggcacgaa gctgctgcaa ctgctgacgc tcaaccagcc
     8701 gatctacaac ccggcgacca acaccttcgc cgcgcccttc gcgccgttca tctcggcgca
     8761 gaagaacgcg accggcggcc ttcaggccgt ctcgaccgac tcgaactcga actacaactc
     8821 gcttcaggtg agcgtcacca agcgcttcag ccgcggcctc cagttcctcg ccgcctacac
     8881 ctacggcaag tccatcgact actactcggg cggcccggtc aacgagctgg tctcgacgcc
     8941 cggcgaccag cgcgactgga agtcgaaccg cgggttatcg gatttcgacc gccggcaccg
     9001 cttttcggtg agctattctt acgcgatccc ggccttttca acttcacgat tcgtgcaggg
     9061 ctgg
//
LOCUS       DBNGOH010001008         2703 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4551030, whole genome shotgun sequence.
ACCESSION   DBNGOH010001008 DBNGOH010000000
VERSION     DBNGOH010001008.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2703)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2703)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2703
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4551030"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..530
                     /locus_tag="AC159P_19635"
     CDS             <1..530
                     /locus_tag="AC159P_19635"
                     /inference="COORDINATES: protein motif:HMM:NF051698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HIR3697708.1"
                     /translation="VYDLPAVVSQDTSARWLLNNWQVNGILTLQSGLPFSVIQVPDNN
                     TVQRANFRPGVTGDIDFDGRTQGRLNQYFNTAAFAPSTLTGGFFDPNNPFGNTGRNIL
                     TGPGQKNLDFSVVKFIPFTERVRGEFRTEFFNLFNWANFANPNANISVPATFGRITQT
                     SAGPRVIQFAFKLNF"
     gene            664..1482
                     /locus_tag="AC159P_19640"
     CDS             664..1482
                     /locus_tag="AC159P_19640"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR3697709.1"
                     /translation="MPDIQLNDVRLYYETHGEGEPLVLVPGFGNGLWIWFKQVSALAR
                     RFRVIAFDPRGVARSEGRDEPLTVRTIAGDVAALLSSLGVESAHVVGASFGGFVAQEF
                     ALLHPRMTRTLVLACTSHGGAGHVRPTAATLAAIASTKGLNTEERVRENLLLAFSPGY
                     VERERAEAARVGALRAQSPVPEYVYRRQLEAAVGFDAARRVSKIEAPTLVITGDADQI
                     VPPENSYNLAALIPRAELRTIAGGSHTFFIERADEFNRAVVDFIERNQSQKSEA"
     gene            1629..2618
                     /locus_tag="AC159P_19645"
     CDS             1629..2618
                     /locus_tag="AC159P_19645"
                     /EC_number="2.3.1.-"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009196163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP synthase III family protein"
                     /protein_id="HIR3697710.1"
                     /translation="MTYARIAGTGSYVPEKVITNADLSRMLGEDIDDFVSRVVGIRER
                     HVCADDESTADLATEAARRALAAAGVAAADLDLIVLATDTPEYLSPATSVVVQHRLGA
                     RRAGTFDVNCACAGFVTALDAASKYVIADAAYRNVLVVGAYAMSKYLDWTEKKTATIF
                     ADGAGAVVLRASDERPGFLASKLVADGSYHGHMGVYAGGTRMPVTEEVLREGRFTKVR
                     FVRKYPPEVNIEGWPLIVGDVLAKANLKKEDVALFLFTQVNLSTIVEVMKRLELPMER
                     THTIMQKWGYTGSACIPMALDDAVREGKLKPGDVVVMCASGGGLNMACAAFRM"
BASE COUNT          482 a          930 c          811 g          480 t
ORIGIN      
        1 tcgtctacga cctgcccgcc gtcgtctcgc aggacacctc tgcgcgctgg ctcctcaaca
       61 actggcaggt caacggcatc ctcaccttgc agtcgggcct ccccttctcg gtcatccagg
      121 tgcccgacaa caacaccgtc cagcgcgcca acttccgtcc cggcgtcacc ggcgacatcg
      181 acttcgacgg gcgcacgcaa ggtagactga atcaatactt caacaccgcg gcgttcgccc
      241 cctcgacgct caccggcggc ttcttcgacc ccaacaaccc gttcggcaac acgggccgca
      301 acatcctgac cgggccgggc cagaagaacc tcgacttctc ggtggtgaaa ttcatcccct
      361 tcaccgagcg cgtgcgcggc gagttccgca ccgagttttt caacctcttc aactgggcca
      421 acttcgccaa cccgaacgcc aacatctccg tgcccgcgac cttcggtcgc atcacgcaga
      481 ccagcgccgg gccgcgcgtc atccagttcg ctttcaagct caatttctga aatagtaggc
      541 agtaggcggt aggtagtagg cagttaaaga ggatgcggat tagaatttga aaaaactcaa
      601 ggttgagatt catgcttcct cttcttactg cctactgcct accgcctact gcctattgct
      661 cttatgcctg acattcaact caacgacgtg cggctgtatt acgagacgca cggcgagggc
      721 gagccgctgg tgctcgtgcc gggcttcggc aacgggctgt ggatttggtt caagcaggtg
      781 tcggcgctgg cgcggcgctt tcgggtcatc gccttcgacc cgcgcggggt ggcgcgctcc
      841 gaggggcgcg acgagccgct cacggttcgc accatcgcgg gcgacgtggc cgcgctgctc
      901 tcatcgctcg gcgtcgagag cgcgcacgtc gtcggcgcct cgttcggcgg cttcgtcgcg
      961 caggagttcg cgctgctgca tccgcggatg acgcgcacgc tcgtcctcgc ctgcaccagc
     1021 cacggcggcg cgggccacgt ccggcccacg gcggcgacgc tcgcggccat cgcctcgacg
     1081 aaggggctga acacggagga gcgcgtgcgc gagaacctgc tcctcgcctt ctcgcccggc
     1141 tacgtcgagc gcgagcgcgc agaggccgcg cgcgtcggcg ccctgcgcgc gcagagtccc
     1201 gtgcccgagt acgtctaccg ccgccagctc gaagccgccg tcggcttcga cgccgcgcgc
     1261 cgcgtctcga agatcgaggc gccgacgctc gtcatcaccg gcgacgcaga ccagatcgtg
     1321 ccgcccgaaa actcttacaa cctcgccgcc ctcatccccc gcgccgagct gcgcaccatc
     1381 gcggggggca gccacacttt cttcatcgag cgcgccgacg aattcaaccg cgcggtcgtg
     1441 gacttcatcg aacgaaatca aagtcagaag tcagaagcat gaagcatgag tcgttatcaa
     1501 attcagatga tgcatgaagg gtagtttcag tcattagatt caggggtttt cagacttcat
     1561 gtttcaaact tcatacttca tgcttctgac ttctgactta tggattcccg actccagact
     1621 gttttcttat gacctacgca cgcatcgccg gcacgggttc ctacgtgccg gaaaaagtaa
     1681 tcaccaacgc ggacctcagc cggatgctcg gcgaggacat cgacgatttc gtctcgcgcg
     1741 tggtcggcat ccgcgagcgc cacgtctgcg ccgacgacga gagcaccgcc gacctcgcga
     1801 ccgaggccgc gcgccgcgcg ctcgcggccg ccggcgtcgc ggccgcggac ctcgacctca
     1861 tcgtgctcgc caccgacacg cccgagtatc tttcgcccgc cacgtccgtc gtcgtccagc
     1921 accgcctcgg cgcgcggcgc gcgggcacct tcgacgtgaa ctgcgcgtgc gcaggcttcg
     1981 tcaccgcgct cgacgcggcg tcgaagtacg tcatcgccga cgcggcctac cggaacgtgc
     2041 tcgtcgtcgg cgcctacgcg atgagcaagt acctcgactg gacggagaag aagacggcga
     2101 ccatcttcgc cgacggcgcg ggcgcggtcg tgctgcgcgc gtcggacgag cggcccggct
     2161 tcctcgcctc gaagctcgtc gccgacggca gctaccacgg ccacatgggc gtctacgcgg
     2221 gcggcacgcg catgcccgtc accgaagagg tcttgcgcga gggccgtttc acgaaagtcc
     2281 gcttcgtgcg gaagtacccg cccgaggtca acatcgaggg ctggcccttg atcgtcggcg
     2341 acgtgctggc gaaggccaat ctcaagaaag aagacgtggc gctctttctc ttcacgcagg
     2401 tcaacctgag caccatcgtc gaggtgatga agcggcttga gctgccgatg gaacggacgc
     2461 acaccatcat gcagaagtgg ggctacaccg gctcggcctg catcccgatg gcgctcgacg
     2521 acgccgtgcg cgaaggcaaa ttgaaaccgg gcgacgtggt cgtcatgtgc gcctcgggcg
     2581 gcggcctcaa catggcgtgc gccgccttca gaatgtgagc ggtgagcagt aagcagtgag
     2641 cagttgagga aaaagcagac tgctcgggat atccttcttt cactgctcac tgcttactgc
     2701 tca
//
LOCUS       DBNGOH010001009         1994 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4917253, whole genome shotgun sequence.
ACCESSION   DBNGOH010001009 DBNGOH010000000
VERSION     DBNGOH010001009.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1994)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1994)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1994
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4917253"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(174..770)
                     /gene="nusB"
                     /locus_tag="AC159P_19650"
     CDS             complement(174..770)
                     /gene="nusB"
                     /locus_tag="AC159P_19650"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HIR3697711.1"
                     /translation="MGLRRKARECALQMLFAADVTGASRAGEAGGVEALSADAPRVDA
                     LVRAYWDELGDPEIDGAAREFATRLAAGALARVNELDERIRSRAEHWRISRMAVVDRN
                     ILRLAVYEFLHEPTPRTVAINEALEIARRFSTYEATQFINGILDAIKRDLDKEFPQEE
                     AAAEAHPDEEPPGQPPAADEDTHEEDDGDEQQAISSGQ"
     gene            complement(849..1334)
                     /gene="ribH"
                     /locus_tag="AC159P_19655"
     CDS             complement(849..1334)
                     /gene="ribH"
                     /locus_tag="AC159P_19655"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HIR3697712.1"
                     /translation="MQPVTHQGRLNAEGFRFAIISSRWNDFITARLVEGALDALERLG
                     AAESAVAHFRVPGSFEIPLLALRLAESEKYDAVICLGTVIRGQTPHFEYVAGEVTKGV
                     AQAAMETGVPVVYGIVTADTLEQAVDRAGVKAGNKGFEAAMAAVELVNLYKEAFQSDE
                     D"
     gene            complement(1473..>1994)
                     /locus_tag="AC159P_19660"
     CDS             complement(1473..>1994)
                     /locus_tag="AC159P_19660"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HIR3697713.1"
                     /translation="QAVCDAIEGCGVPVVCALWGRVVGVGCELAFACHLRVAAGDAVF
                     RLSEHNAAHCSTDAARHRLALIAGPALAAIPGHEHVIAGETLLRLFVNLVVTREEVLA
                     AAEQLARTIIDNGAPLAARACLEAVARGARLPLAEGLNLEAELFSRLFSTADAREGLA
                     AFLEKRKPVFKGE"
BASE COUNT          343 a          656 c          656 g          339 t
ORIGIN      
        1 cctcggcctc ggccagaaac tcgctgagca gttcgtcgtc cataagaaca gcaggcagta
       61 ggcagcgggg agcgggcagt taaaaaactt cgcgtggaaa aacttcgagc ggtgcgccgc
      121 cgtcgaaagc agccacaccg ctcatcagac tactaccgcc tactgcctac tgcctactgc
      181 ccgctgctta ttgcctgctg ctcgtcgccg tcgtcctctt cgtgcgtgtc ttcgtcggcg
      241 gcgggtggct ggccgggcgg ctcttcgtcg gggtgggctt cggcggcggc ctcttcctgc
      301 gggaactctt tgtcgaggtc gcgcttgatg gcgtcgagga tgccgttgat gaactgcgtc
      361 gcctcgtagg tcgagaagcg gcgcgcgatc tccagcgcct cgttgatggc gacggtgcgc
      421 ggcgtcggct cgtgcaaaaa ttcgtagacg gcgagacgca ggatgttgcg atccacgacc
      481 gccatccgcg agatgcgcca gtgctctgcg cgcgagcgga tgcgctcgtc cagctcattc
      541 acgcgcgcga gcgcgcccgc cgccagccgc gtcgcgaact cgcgcgccgc cccgtcgatc
      601 tccgggtcgc ccagctcgtc ccagtacgcg cgcaccagcg cgtccacgcg cggcgcgtcg
      661 gcactgagag cctccacgcc gcccgcctcg cccgcacgcg acgcgcccgt gacatctgcc
      721 gcaaagagca tctgcaaggc gcattcacgc gccttacgtc ttaaacccat atgatgaata
      781 cacagtgatg aggactgagg actgaggact gagtaaaagc atgatttctt tcactcggtc
      841 ctcagtcctc agtcctcatc actttggaaa gcttccttgt aaaggttgac caactcgacc
      901 gccgccatcg cggcctcgaa gcctttgttg cccgccttga cgcccgcgcg atcaaccgcc
      961 tgctccagcg tgtccgcggt gacgatgccg tagacgacgg gcacgcccgt ctccatcgcc
     1021 gcctgcgcca cgcccttcgt gacctcgccc gcgacgtact cgaagtgcgg cgtctgcccg
     1081 cgaatcaccg tgcccagaca gatcaccgcg tcgtacttct cactttcggc gagccgcagc
     1141 gccagcagcg gtatctcgaa cgagccgggc acgcggaagt gcgcgaccgc gctctcggcg
     1201 gcgccgagcc gttcgagcgc gtcgagcgcc ccctcgacca gccgcgccgt gatgaagtcg
     1261 ttccaccggc tcgaaatgat cgcgaagcga aacccctcgg cgttcaagcg cccctgatgt
     1321 gtgacgggct gcaaggtttt ctgtcgcgtc cccgaatctg tttgagagtt aataagcaag
     1381 ggcagtatag aaaaggcggt gacgaatgac aagaagtgaa cagtgcgcgg tgagcaatca
     1441 gcggtaaaag agagaaagaa actgctcgcc gctcactctc ccttgaaaac cggcttgcgt
     1501 ttttcgagga aggcggcgag tccctcgcgg gcgtcggcgg tggagaagag gcggctgaag
     1561 agttcggctt cgaggttcaa gccttccgcg agggggaggc gcgcgccgcg ggcgacggct
     1621 tcgaggcagg cgcgtgcggc gagcggggcg ccgttgtcga tgatcgtgcg ggcgagttgt
     1681 tcggcggcgg caagaacctc ttcgcgtgtg acgacgaggt tgacgaagag tcggagcagg
     1741 gtttcgcccg ctatgacgtg ttcgtggccg ggtatcgcgg cgagcgcggg gccggcgatg
     1801 agcgcgagcc ggtgccgcgc cgcgtcggtc gagcagtggg cggcgttgtg ctcggagagg
     1861 cggaacacgg catcgcccgc cgcgacgcgg aggtggcacg cgaacgcgag ttcgcagccg
     1921 acgcccacga cccggcccca caacgcgcag acgaccggca cgccgcaccc ctctatcgcg
     1981 tcgcaaacgg cctg
//
LOCUS       DBNGOH010001010         2409 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_884667, whole genome shotgun sequence.
ACCESSION   DBNGOH010001010 DBNGOH010000000
VERSION     DBNGOH010001010.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2409)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2409)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2409
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_884667"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(262..831)
                     /locus_tag="AC159P_19665"
     CDS             complement(262..831)
                     /locus_tag="AC159P_19665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697714.1"
                     /translation="MKFCKVCGANLYAVRQAVDARETGEKIDWGKTWVAEMFLSGQEI
                     QRRKEEDERRRGITPEVKRYNEIKAGVITGCVGVGVAIFLLVFMQGIILGGNVSASAA
                     EILSRLWIAGVIPLFVGIALVINGLFVSKRLVEIAGPNAPERAGLTGPDREGEPRSLR
                     PADTNEFIPSGFSVTEETTKHLRDSGQKR"
     gene            1114..1911
                     /locus_tag="AC159P_19670"
     CDS             1114..1911
                     /locus_tag="AC159P_19670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014810658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3697715.1"
                     /translation="MSADPAAPKEERLRPATARALPLVALLVALAAWAALAALGVFPG
                     SVFPSPLAVARGFAEEVRTGRLPNDLVASLFRVTTGFALAVALGLPLGLWLGHSARAR
                     AALLPAVNFFRSLSPLAWIPFAILWFGIGDVPAIFLIFMASFFPVVLATVAAVASVPA
                     VYYRVAGDYGLRGAELLTRVTLPAIAPQVITALRVTAGLAWLVVVVAEMIAGRDGLGF
                     AIWDARNGLRMDLLVSGMIVIGLIGVVIDRLLVRLMSIPSVRWGYER"
     gene            1908..>2409
                     /locus_tag="AC159P_19675"
     CDS             1908..>2409
                     /locus_tag="AC159P_19675"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3697716.1"
                     /translation="MSELRLENVSHSFGAVEVFRGLSLEIARGEFVAVVGPSGCGKTT
                     LLGLCSGFLKPTSGRVECAGRVRTVYQQDGLFPWLTVAQNIALGLRRVRDRAEHDARL
                     AEMIALIRLEGFAAHYPHQLSGGMRQRVELARALAGDTDILLLDEPFSSVDYLTRLRL
                     RGELARM"
BASE COUNT          311 a          835 c          829 g          434 t
ORIGIN      
        1 cagtttgccg ccgtgttcgc tagaaacgag cgtaagatgg ggcatttggc ggaagtcgca
       61 tacgaatgaa ccccgctata acagcttcct ggtcggagat atgcgggcgg aggcgaactt
      121 gcgcgccagc accaggctca gcgcgcccag caaacgcggc ccctcgccga ggctcgagac
      181 gatcacccgc ggcgacggca ggccgcggca cgcgaggcgc gtgcgagtaa gtgccgtcgc
      241 cgttcgggtg tggtgcgggg cttagcgctt ctggccggaa tccctgaggt gcttggtcgt
      301 ctcttccgtg acgctgaagc cggaggggat gaattcgttc gtgtcggcgg ggcgcagcga
      361 gcgcggctcg ccctctctgt ccgggccggt cagccccgct ctctccgggg cgttcggccc
      421 cgcgatttcg accagtctct tactcacgaa gaggccgttg atgacgagcg cgatcccgac
      481 gaacagtgga atcaccccgg caatccacag gcggctgagt atctccgccg cgctcgcgga
      541 gacgttgccg ccgaggatga tgccctgcat gaagaccagg agaaagatcg ccacccccac
      601 cccgacgcag cccgtaatca cgccggcctt gatttcgttg tagcgcttca cctcgggcgt
      661 gatgccccgc cgccgctcgt cctcttcctt gcgcctttga atctcctgcc cggagaggaa
      721 catctccgcg acccacgttt tgccccagtc tattttctcg cccgtctcgc gggcgtccac
      781 cgcctgtcgc acggcgtaga ggttcgcccc gcacacttta cagaacttca actcttcgct
      841 ttgctgcgcg ccacaccgcg gacagaacat ggcatctcct ctcgtgcgtt tgcaggggga
      901 tacgacaacc gcaaggccga ggttcttgac tcggcgcgcc gtgcgccacg agggcgcggc
      961 ggcgacggcc cctatcatac aggcagccgt tggtagacgg caggctccgc ggtatagaat
     1021 gctgacgccg acacgctcga cgattcatcg aacgccgacg cttcggacgc cgaggattcg
     1081 ccggcccgag gtttctccgc cgcccgcttc tttatgtccg ccgaccccgc cgccccgaaa
     1141 gaagagcgcc tgcgcccggc gacggcgcgc gcgctgccgc tcgtcgcgct gctcgtcgcg
     1201 ctcgcggcgt gggccgcgct ggcggcgctc ggcgtcttcc ccggctcggt cttcccttcg
     1261 ccgctcgcgg tggcgcgcgg cttcgcagag gaggtgcgca cggggcggct gccgaacgat
     1321 ctggtggcct cgctcttccg cgtgacgacg ggcttcgcgc tggccgtcgc gctgggcctg
     1381 ccgctgggcc tgtggctcgg gcacagcgcg cgggcgcggg cggcgctgct gccggcggtc
     1441 aacttcttcc gcagcctctc gccgctggcg tggataccgt tcgccatcct ctggttcggc
     1501 atcggcgacg tgccggccat cttcctcatc ttcatggctt cgttcttccc cgtcgtgctg
     1561 gcgaccgtgg ccgcggtggc gagcgtgccg gcggtctact atcgcgtcgc aggggactac
     1621 ggcctgcggg gcgcggagct tttgacgcgg gtcacgctgc cggccatcgc gccgcaggtg
     1681 atcacggcct tgcgcgtgac ggcgggactg gcgtggctcg tcgtggtcgt ggcggagatg
     1741 atcgcggggc gcgacgggct gggcttcgcc atctgggacg cgcgcaacgg cctgcggatg
     1801 gacttgctcg tctcggggat gatcgtcatc ggcttgatcg gcgtcgtcat cgaccgcctg
     1861 ctcgtgcggc tgatgagcat cccgagcgtc cgctgggggt acgagcgttg agcgagctgc
     1921 gactcgaaaa cgtcagccac tcgttcggcg cggtcgaggt cttccgtggg ttgtcgctgg
     1981 agatcgcgcg gggcgagttc gtggcggtcg tcggcccctc gggctgcggc aagacgacgc
     2041 tgctcggcct ctgctcgggc ttcctcaagc ccacttccgg cagggtcgag tgcgcgggcc
     2101 gcgtgcgcac ggtctaccag caggatgggc tgttcccgtg gctgaccgtg gcgcagaaca
     2161 tcgcgctcgg gctgcgccgc gtccgcgacc gcgccgagca cgacgcgcgt ctcgccgaga
     2221 tgatcgcgct catccgcctc gaaggcttcg ccgcgcacta cccgcaccag ctctcgggcg
     2281 gcatgcgcca gcgcgtcgag ctggcgcgcg cgctcgcggg cgacacggac atcctgctgc
     2341 tcgacgagcc gttctcgtcg gtcgattacc tgacgcggct gcgcctgcgc ggcgagctgg
     2401 cgcggatgc
//
LOCUS       DBNGOH010001011         1831 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1070521, whole genome shotgun sequence.
ACCESSION   DBNGOH010001011 DBNGOH010000000
VERSION     DBNGOH010001011.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1831)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1831)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1831
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1070521"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..203
                     /locus_tag="AC159P_19680"
     CDS             <1..203
                     /locus_tag="AC159P_19680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HIR3697717.1"
                     /translation="LSRANLLTQFAVLYGDPNLINTVPERVAAVTKEDVQRVAQKYLK
                     QTNRTVVVTMPKAREQRTAVGQ"
     gene            218..1771
                     /locus_tag="AC159P_19685"
     CDS             218..1771
                     /locus_tag="AC159P_19685"
                     /inference="COORDINATES: protein motif:HMM:NF017044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HIR3697718.1"
                     /translation="MKNRTMTKTILGATAARTLLAAALLVGSAAPAARAQTPGPQQPP
                     AGPADEGLKGVVLKGRAPVSREVLKVKLPKAQEATLKNGLRVVLLENHRVPSFSLQLV
                     VLSGGLSDPAEHKGLAQFTAALMREGTAKRTSRQIAEELDTMGASVFAASNLSGFTST
                     VAASGLVENLDPVLDIFADVARNPSFPKDEVEKYKTRTAAQLQQQRQSPQFLAAERFN
                     RAVYGDHPASLLAAPPENLKRTTPEDLARFHQTYYRPNNAMLAVVGDVTMKELLPKLE
                     RAFGDWKQGEVPKTTIPAATAHYKEPRIYLIDRPGSVQTLLQIGNIGIERTDPDYYAL
                     LVMNRILGGGGTARLQRNLREDKGYTYGASSGFGGAKFRGVFAANSSVRTEVTEGAMR
                     EFLNEFKRIRDEKVSAFDLDNAKRGLTGSFALSLEQPQLLLANIVTQKLYDLPADYWD
                     TYPQRVAAVTADDVQRVARKYVDLDRLQVVAVGDAAKVRQALAPYGTVEVYDTNGKPV
                     AEAAKPGTN"
BASE COUNT          364 a          656 c          582 g          229 t
ORIGIN      
        1 cgctcagccg cgccaacctg ctcacgcagt tcgcagtcct ctacggcgac ccgaacctca
       61 tcaacacggt gcccgaaagg gtcgccgccg tgacgaagga ggacgtgcag cgcgtcgcgc
      121 agaagtacct gaagcagacc aaccgcaccg tcgtcgtcac gatgccgaag gcgcgcgagc
      181 agagaacagc cgtcgggcag taaggagaga acgagagatg aaaaacagga cgatgacgaa
      241 aacgattctc ggcgcgacgg cggcgcggac gctgctcgcc gccgcgctcc tcgtcggctc
      301 ggcggcgccc gccgcccgcg cgcaaactcc ggggccgcag cagccgcccg ccggccccgc
      361 ggacgaaggc ttgaagggcg tcgtgctcaa agggcgcgcg cccgtcagca gggaagtgct
      421 gaaggtcaaa ctgccgaagg cgcaggaggc gaccttgaag aacggcctgc gcgtcgtgct
      481 gcttgagaac caccgcgtcc cgagcttctc cttgcagctc gtcgtgctga gcggcggcct
      541 ctccgacccg gcggagcaca agggtctggc gcagttcacc gccgcgctca tgcgcgaggg
      601 cacggcgaag cgcacgagcc gccagatcgc cgaagagctg gacacgatgg gcgcgagcgt
      661 cttcgccgcg tcgaacctct cgggcttcac gagcacggtc gcggcgagcg gcctcgtcga
      721 aaacctcgac cccgtgctcg acatcttcgc ggacgtggcg cgcaacccct ctttcccgaa
      781 ggacgaggtc gagaagtaca agacgcgcac cgccgcgcag ctccagcagc agcgccagag
      841 tccgcagttc ctcgccgccg agcgcttcaa ccgcgccgtc tacggcgacc acccggcctc
      901 cctgctcgcc gcgcccccgg agaacctcaa gcgcacgacg ccggaagacc tcgcgcgctt
      961 tcaccagacg tactaccgcc cgaacaacgc gatgctcgcg gtggtcggcg acgtgacgat
     1021 gaaggagctg ctgccgaagc tcgaacgcgc cttcggcgac tggaagcagg gcgaggtgcc
     1081 gaagaccacg atccccgcgg ccaccgcgca ctacaaagag ccgcgcatct acctcatcga
     1141 ccggcccggc tccgtccaga ccttgttgca gatcggcaac atcggcatcg agcgcacaga
     1201 ccccgactac tacgcgctgc tcgtcatgaa ccgcatcctc ggcggcggcg gcaccgcgcg
     1261 cctgcaaagg aacctgcgcg aggacaaggg ctacacctac ggcgcgtcga gcggcttcgg
     1321 cggcgccaag ttccgcggcg tcttcgccgc caactcttcg gtgcgcaccg aagtgaccga
     1381 gggcgcgatg cgcgagttcc tgaacgagtt caagcgcatc cgcgacgaga aggtctccgc
     1441 cttcgatctg gacaacgcca agcgcggcct caccggcagc ttcgcgctct cgctcgaaca
     1501 gccgcagctc ctgctggcga acatcgtcac gcagaagctc tacgacctgc ccgccgatta
     1561 ctgggacacc tacccgcagc gcgtcgccgc cgtcaccgcc gacgacgtgc agcgcgtcgc
     1621 gcgcaagtac gtcgatctcg accgcctaca ggtcgtcgcc gtcggcgacg ccgcgaaggt
     1681 gcgacaggcg ctcgcgccat acggcaccgt cgaagtctac gacaccaacg gcaagcccgt
     1741 ggccgaggcc gcgaagcccg gcacgaatta agaagtaggc ggtaggcagt taaacagaag
     1801 acagaggtca gaagtcggaa gtcagaaaga c
//
LOCUS       DBNGOH010001012         3183 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1070666, whole genome shotgun sequence.
ACCESSION   DBNGOH010001012 DBNGOH010000000
VERSION     DBNGOH010001012.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3183)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3183)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3183
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1070666"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..412
                     /locus_tag="AC159P_19690"
     CDS             <1..412
                     /locus_tag="AC159P_19690"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HIR3697719.1"
                     /translation="DVRLRDEREAVRRVERWGRLALEACKQSGRARVPAVEAPAALAA
                     LCEKVDAGGGAARLMFAERGGVGLGEVAATLDARPASVIALVGPEGGWDDEEIERCAR
                     EGWAVVTLGGRTLRAETAAITVAALLQHLFGDLK"
     gene            649..924
                     /locus_tag="AC159P_19695"
     CDS             649..924
                     /locus_tag="AC159P_19695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697720.1"
                     /translation="MAQSRDEAVARAKADLAKRLGVAEGEVREEAVEEADFPDGALGA
                     HVRGEMSTMMITSGWRIRLGAGGKSYEYRADRNQLRLYKYKGVNHKI"
     gene            1002..2456
                     /locus_tag="AC159P_19700"
     CDS             1002..2456
                     /locus_tag="AC159P_19700"
                     /inference="COORDINATES: protein motif:HMM:NF012311.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HIR3697721.1"
                     /translation="MNRQNLVVHGALALTLVLAAAFVGQVQRWNGRQRVRESRVRQDV
                     VLGSAEEIVVAPPRAERRTGDEPEVLVRFRAGTTSDEIERIAGRLNDRVEDEIESVRG
                     LVAIDDRDGLTAAMVAGQYSGLAEVEYVEENYDGWSSKPLPGRRVVGGTFAAPVAGPN
                     DPMFAEQWALSNDGRREGKSEADIAALTAWAKTHGNSKVVVAVLDSGVDYTHKDLAEN
                     MWIRPAGIDPYVDEQLGEIDDLHGFDAVAADGLPDPMDENGHGTHCAGIIGAVGNNGE
                     GIAGVNWKIEVMPLRFMSAGGFGTTKDAIEAINYVIDRKRAGVNVRVISASWGSTRRS
                     QALEDVIRKAGEEGIIFIAAAGNSSVNTDTNPHYPSSYKLPNVMSIAALDRRDQLAGF
                     SNFGAKSVHLAAPGKEILSTWLRDAYEEHSGTSMATPVVSGVAALILSCEPELKVEQL
                     KQRLMDTADKLDALNGKTVSGGRVNAARAVGAVK"
     gene            2715..>3183
                     /locus_tag="AC159P_19705"
     CDS             2715..>3183
                     /locus_tag="AC159P_19705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020374456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HIR3697722.1"
                     /translation="MSASTPTKETTTELDQLCINTIRALSIDAVQRAESGHPGLPLGA
                     APMAYVVWTRFMRHNPRNPKWQGRDRFLLSAGHGSMLLYSLLHLTGYDLPLEELKNFR
                     QWGSKTPGHPENILTPGVEITTGPLGQGFANGVGMAMGAAHLAAKFNREGFPLV"
BASE COUNT          578 a         1028 c         1095 g          482 t
ORIGIN      
        1 cgacgtgcgg ctgcgcgacg agagggaggc ggtgcggcgc gtcgaacgct gggggcggct
       61 cgcgctcgaa gcgtgcaagc agtctgggcg tgcgcgcgtc cccgccgtcg aagcgcccgc
      121 cgcgctcgcg gcgctgtgcg agaaggttga cgcgggcggc ggggcggcgc gtctcatgtt
      181 cgcggagcgc ggcggcgtcg gtctgggcga agtcgccgcg acgttggacg cgcgccccgc
      241 atccgtcatc gccctcgtcg ggccggaggg cggttgggac gacgaggaga tcgagcgatg
      301 cgcgcgcgaa ggctgggccg tcgtcacgct cggcggtcgc accctccgcg cggagacggc
      361 ggccatcacc gtcgccgctc tgctccaaca cctgttcgga gacctcaagt gatttcggat
      421 ttcggatttc ggattgaccg agtaaaggaa gttgaatcct cgaaatcacg cttcaagctc
      481 aagcggcacg ccaatccgca atccgcaatc cttccgccgc tcactttctt ttgccttttt
      541 tttcgggcgg gcgcagaata tcgttgtggg catcgaaagg gtaaacgagt gggcggcccg
      601 gcgcgtataa agacggcaga gattctttcg acgaagggcg ggccgaggat ggcgcagagc
      661 agggacgagg cggtggcgag ggcgaaggcc gatctggcga agcgcctcgg cgtggccgag
      721 ggcgaggtca gagaggaggc ggtcgaggag gcggacttcc ccgacggcgc gctcggcgca
      781 cacgtccgcg gcgagatgag caccatgatg attacctcgg gctggcgcat ccgcctcggc
      841 gcgggcggca agtcctacga ataccgggcc gaccgcaatc agttgcggct gtacaaatac
      901 aagggcgtga accacaagat ttgaaagtct ggagtctgga gtcaggacaa aaagcagttg
      961 gttctgactc cagactccag actaaacaag gagttgaagc gatgaaccgg cagaatttgg
     1021 tcgtccatgg ggcgttggcg ttgacgttgg tgttggccgc cgctttcgtc ggccaggtgc
     1081 agcgttggaa cggcaggcag cgcgtgcgcg agtcgcgcgt caggcaggat gtcgtgctcg
     1141 ggagtgcgga ggagatcgtc gtcgcgccgc cgcgcgccga gaggcgcacg ggcgacgagc
     1201 cggaggtgct cgtgcgcttc cgcgcgggca cgacctccga cgagatcgag cgcatcgcgg
     1261 gtcggctcaa cgaccgcgtc gaggacgaga tcgagtcggt gcgcggcctc gtcgccatcg
     1321 acgaccgcga cggcctgacc gccgcgatgg tcgcgggcca gtacagcggc ctcgccgagg
     1381 tcgagtacgt cgaggagaac tacgacggct ggtcttccaa gccgctgccg ggccgccgcg
     1441 tcgtgggcgg caccttcgcc gcgcccgtcg ccggcccgaa cgacccgatg ttcgccgagc
     1501 agtgggcctt gagcaacgac gggcggcgcg aaggcaagtc ggaggcggac atcgccgccc
     1561 tcacggcgtg ggccaagacg cacggcaaca gcaaggtggt cgtcgccgtc ctcgacagcg
     1621 gcgtggacta cacgcacaag gacttggccg agaacatgtg gatcaggccc gccggcatcg
     1681 acccctacgt ggacgaacag ctcggcgaga ttgacgacct ccacggcttc gacgcggtgg
     1741 cggcggacgg gctgcccgac ccgatggacg agaacggcca cggcacgcac tgcgcgggca
     1801 tcatcggcgc ggtcggcaac aacggcgagg gcatcgcggg cgtcaactgg aagatcgagg
     1861 tgatgcccct gcggttcatg tcggcgggcg gcttcggcac cacgaaggat gccatcgagg
     1921 cgatcaacta cgtcatcgac cgcaagcgcg cgggcgtcaa cgtccgcgtc atcagcgcga
     1981 gctggggttc gacgcgccgc tcgcaggcgc tcgaagacgt gatccgcaag gcgggcgagg
     2041 agggcatcat cttcatcgcc gccgccggca actcctcggt caacaccgac accaacccgc
     2101 actacccttc gagctacaag ctgccgaacg tgatgagcat cgccgcgctc gaccgtcgcg
     2161 accagctcgc cggcttctcg aacttcggcg cgaagagcgt ccacctcgcc gcgcccggca
     2221 aggaaatcct gagcacgtgg ctgcgcgacg cctacgaaga gcactcgggc acctcgatgg
     2281 cgacgccggt cgtctccggg gtggccgcgc tcatcctctc ctgcgagcct gagctgaagg
     2341 tcgaacagct caagcagcgc ctgatggaca ccgccgacaa gctcgacgcg ctcaacggca
     2401 agaccgtcag cggcggtcgc gtcaacgccg cccgcgccgt cggcgccgtg aagtaagaca
     2461 aagcagtagg cggtaggcag taggcagtag gcagcaaaag caagaggctt gagatataac
     2521 gtctcaagcc tcttgcttca ttgcatgaag agcggagagg cttggcactt tacctcccaa
     2581 gcttcttcgc tcttctgtct tgactgccta ctgcctactg cctactgcct tttcccttgc
     2641 gctcggggcg cgggcgcgat aaaacgtgtg ggcgaaatct cctttatcaa aaatcgaatc
     2701 gagggtatcg acagatgagc gcgtccacgc cgacgaaaga gaccacgacc gaactcgacc
     2761 agctctgcat caacacgatc cgcgcgcttt ccatcgacgc cgtccagcgc gccgagtcgg
     2821 gccatccggg cctgccgctc ggcgccgcgc cgatggccta cgtcgtgtgg acgcgcttca
     2881 tgcgccacaa cccgcgcaac ccgaagtggc aggggcgcga ccgcttcctc ctgagcgcgg
     2941 gccacggctc gatgcttctc tactccctcc tgcacctgac cggctacgac ctcccgctcg
     3001 aagagctgaa gaacttccgc cagtggggct cgaagacgcc ggggcacccg gagaacatcc
     3061 tgacgccagg cgtcgagatt acgaccggcc cgctcggtca gggcttcgcc aacggcgtcg
     3121 gcatggcgat gggcgcggcg cacctcgcgg cgaagttcaa ccgcgaaggg ttcccgctcg
     3181 tcg
//
LOCUS       DBNGOH010001013         5384 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1804413, whole genome shotgun sequence.
ACCESSION   DBNGOH010001013 DBNGOH010000000
VERSION     DBNGOH010001013.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5384)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5384)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5384
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1804413"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(34..1887)
                     /locus_tag="AC159P_19710"
     CDS             complement(34..1887)
                     /locus_tag="AC159P_19710"
                     /inference="COORDINATES: protein motif:HMM:NF015140.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007154 - cell communication [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Calx-beta domain-containing protein"
                     /protein_id="HIR3697723.1"
                     /translation="MSTPRRPARVPRASRRRLLPAAAAALLCALCLFVAPPPTRAQTS
                     TGSPNVVVSQFYSRGGETGAAHPNDFIELFNRGSNTVDLSQYTLQVTSVEGNVTSTIR
                     VSFVSLGGGVPLRPGEYRTVTFMPLPPGPIPLPSALGPVGKIAIIKSREAIPQVDCPL
                     GLAPDIEDYVGYGAATCFEGTGPAPTPTLALATYRNNNGCGDTDNNGADFTLAAAPSP
                     RDSSTGGTPRAPCPLPPGTSTFRFTTSFIVTSENVPSVQVTVTRTGGDNSAPATVDYA
                     TTDGTATERKDYTTAAGTLRFAAGETSKSFDILINNDVFPNEGGGSEFFDLALSNPTG
                     MTALADPSAMRITIDDDDTTTVTANPIDDPTFFVRTHYADFLNRVPDQAGLDFWVGNI
                     TSCGADLQCREARRVNVSAAFFFAIEFQETGYYAYRLYKASFRNSPDNGPRPRGFPTY
                     REFIRDTTDTSRDIIVGVGNWEEQLSINKQALADAFVTRAEFLARFPTTLTPAQYVDA
                     LNLNANNPLSQTERDALVNGLTAGTETRATALRRVADDADFRQAELNRAFVLMQYFGY
                     LRRSPNAAPDTNFDGYDFWLSKLDQFDGNFVEAEMVRAFIESIEYRQRFGQ"
     gene            complement(2124..3305)
                     /locus_tag="AC159P_19715"
     CDS             complement(2124..3305)
                     /locus_tag="AC159P_19715"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008405661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HIR3697724.1"
                     /translation="MAQDIFILGGKRTPMSSYVGELKDVKAIDLGAAAARGALEAAGV
                     AAEEIDHTVFGNALQTSGDAIYGARHVALKAGVPHDRPALTVNRLCGSGIQSVISGAQ
                     MILLGEARTALVGGMESMSMAPHVIYGARSGFALGQGKLEDSLMVALLDTYCNTTMAG
                     TAENLARRYEITREEQDAYALRSQQEAKRAQDAGIFAEEIVPVEVKTRKGAKLVERDD
                     HLRPDTTLEVLARLPTAFSKDGFVTAGNASGIVDGAAALVIAGKEFVKGKNAKPLGRL
                     VSWAYAGVEPDIMGIGPVPAIREALRKANLKLDRIDLVEVNEAFACQYLAVEKELGLD
                     RSKTNVNGGAIALGHPLGASGTRIMLTALLELQRRGGRYGLAAACIGGGQGIAIVFER
                     V"
     gene            complement(3401..4237)
                     /locus_tag="AC159P_19720"
     CDS             complement(3401..4237)
                     /locus_tag="AC159P_19720"
                     /inference="COORDINATES: protein motif:HMM:NF018028.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HIR3697725.1"
                     /translation="MSVAAAAPTDETLVIETPERVPLHFALASIGNRFLACAFDHTLQ
                     VVAIALVVFFFVWLGNAASYYEDLKNAPKWVVALMLVAVFLVWSSYFVLFEWAWRGQT
                     PGKRWLKLRVIREDGRPVTFWEAAARNLLRIFDMMPWLGVPFYSIGLVAVFASRRDQR
                     IGDMVAGTVVVREREAQAPSFEEVFSAPTSDTALRRSFKPVPFSASLAPLTEPEIEVV
                     EAFLRRRWDLLEAPRQWMAWRIATPILYKIRPAFDPDTFTYEGFLEELLHRYRAAHRF
                     TD"
     gene            complement(4336..>5384)
                     /locus_tag="AC159P_19725"
     CDS             complement(4336..>5384)
                     /locus_tag="AC159P_19725"
                     /inference="COORDINATES: protein motif:HMM:NF013080.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HIR3697726.1"
                     /translation="EPLSWRKQLEQQKRDVGLPPEHPTVASLLKAAGYRTALVGKWHL
                     GYLPTYGPVKSGFEEFFGIMSGGATYFTHKDANDEPDFFEGEVPVERAGYMTDLLTER
                     AVEYLGRRRGEERPFYLSLHYTAPHWPWEGPGDAAGAKSGALGYAGMTAGGSLQAYAA
                     MMKSLDDGVGRVLRALKGARLERDTLVVFTSDNGGERFSFNWPFSGKKFELLEGGLRV
                     PAIVRWPGVVPANRVSRQVAVTMDWTATILAAAGAKADPAYPLDGEDLLPVLRGTRAP
                     FERALFWRHNKQSAARRGRWKYYRPDDKSEFLFDLSVDEREQADFKASRRAEFEALRA
                     EYARWEAQMLPPKR"
BASE COUNT          828 a         1709 c         1958 g          889 t
ORIGIN      
        1 cggtgttccg ataagaccgc ctcgcggacg ctcttactgc ccgaagcgct ggcggtactc
       61 gatggactcg atgaaggccc tgaccatctc ggcctcgacg aagttgccgt cgaactggtc
      121 gagcttcgag agccagaagt cgtagccgtc gaagttcgtg tcgggcgcgg cgttcgggct
      181 acggcgtaga tacccgaagt attgcatcag cacgaaggcg cggttgagtt cggcctgccg
      241 gaagtccgcg tcgtcggcca cgcggcggag cgcggtggcg cgcgtctcgg tgcccgccgt
      301 caggccgtta accaaagcgt cgcgctcggt ttgcgagagc gggttgttag cgttcaggtt
      361 gagggcgtca acgtactgcg cgggcgtcag cgtcgtcggg aagcgtgcga ggaattcggc
      421 gcgcgtgacg aaggcgtcgg cgagcgcctg cttgttgatg ctcaactgct cctcccagtt
      481 gccgacgccg acgatgatgt cgcgcgaggt gtccgtcgtg tcgcggatga actcgcggta
      541 ggtcgggaag ccgcgcgggc gcgggccgtt gtcggggctg ttgcggaacg acgccttgta
      601 gagcctgtaa gcgtagtagc cggtctcctg gaattcgatg gcgaagaaga aggccgccga
      661 gacgttgacg cgcctcgcct cgcggcactg taagtctgcg ccgcaggagg tgatgttgcc
      721 gacccagaag tcgaggcccg cctgatccgg cacgcggttg agaaagtccg cgtagtgcgt
      781 gcggacgaag aaggtcgggt cgtcgatggg gttcgcggtg accgtcgtcg tgtcgtcgtc
      841 gtcgatggtg atccgcatcg cggacgggtc ggcgagcgcg gtcatgccgg tcgggttcga
      901 gagcgcgagg tcgaagaact ccgagccgcc gccctcgttg gggaacacgt cgttgttgat
      961 gaggatgtcg aagctcttgc tggtctcgcc cgcggcgaag cggagcgtac cggcggcggt
     1021 cgtgtagtcc ttgcgctcgg tggcggtgcc gtcggtcgtc gcgtaatcga cggtcgcggg
     1081 cgcagagttg tcgccgcccg tgcgcgtgac cgtcacctgc acgctcggca cgttctccga
     1141 agtgacgatg aaggaggtgg tgaatcggaa cgtgctcgtc cccggcggca gcgggcaggg
     1201 cgcgcgcggc gtgccgcccg tgctgctgtc gcgcgggctg ggcgcggcgg cgagcgtgaa
     1261 gtcggcgccg ttgttgtcgg tgtcgccgca gccgttgttg ttcctgtagg tcgccagcgc
     1321 gagcgtcggc gtgggggccg ggccggtgcc ctcgaaacag gtggccgcgc cgtagccgac
     1381 gtagtcctcg atgtccgggg cgaggccgag cgggcaatcg acctgcggga tcgcctccct
     1441 gcttttgatg atggcgattt tgccgacggg gccgagcgcc gagggcagag ggatcgggcc
     1501 gggcgggagc ggcatgaagg tgactgtgcg gtactcgccc ggcctgaggg ggacgccccc
     1561 gccgagcgag acgaaagaga ccctgatggt actggtgacg ttgccctcga ccgaagtgac
     1621 ctgcaaggtg tactgcgaca gatcgacggt gttgctgccg cggttgaaaa gctcgatgaa
     1681 gtcgttggga tgtgcggcgc cggtctcgcc gccgcgcgag tagaactggc tgacgacgac
     1741 gttgggcgac cccgtagagg tctgcgcccg cgtcggcggc ggcgcgacga agaggcagag
     1801 ggcgcagagc agtgcggctg ccgcggcggg caggagccgg cggcgcgagg cgcggggcac
     1861 tcgcgccgga cgacgggggg tggacatggt aatgctccgt gatgccgaag gaaaaaagtg
     1921 tgaaccgctt gagtcggacg cgcgtgactc ggacgcgacg cgtgtgaacc gcttgaagtg
     1981 acgagaagtg aacttgctcc gaagtggctt cgaagtaaag ggacgtaaag gggagacttg
     2041 cgcggcgcgt gcctcgaacc gagggcgttg tcggcgcgcg cacggtaggt tcgtgcacgc
     2101 gccgcgcggt tgtcaagcgc agctcagacg cgctcgaaga cgatggcgat gccctgcccg
     2161 ccgccgatgc aggccgcggc gaggccgtag cgaccgccgc ggcgttgaag ctcaagcagc
     2221 gccgtcagca tgatgcgcgt gcccgaagcg ccgagcgggt ggccgagcgc gatggcgccg
     2281 ccgttgacgt tggtcttcga gcggtcgagg cccaactcct tctcgacggc gaggtattgg
     2341 caggcgaacg cctcgttgac ttcgacgagg tcgatccggt cgagcttgag gttggccttc
     2401 cgaagcgcct cgcggatggc ggggacgggg ccgatgccca tgatgtccgg ctcgacgccc
     2461 gcgtaggccc aactgacgag gcggcccagc ggcttggcgt tcttaccttt gacgaactcc
     2521 ttgcccgcga tgacgagcgc cgccgcgccg tccactatcc cggaagcgtt cccggcggtg
     2581 acgaagccgt ccttgctgaa cgcggtcggc agccgcgcca gaacttcgag cgtcgtgtcg
     2641 ggccgcaggt ggtcgtccct ctcgacgagc ttcgcgccct ttcgcgtctt cacctcgacg
     2701 ggcacgatct cttcggcgaa gattccggcg tcctgcgcgc gcttcgcctc ctgttgcgag
     2761 cggagcgcgt aggcgtcctg ctcttcgcgc gtgatctcgt agcggcgcgc gaggttctcc
     2821 gcggtgccgg ccatcgtcgt gttgcagtag gtgtccaaga gcgcgaccat cagcgagtct
     2881 tcgagcttgc cctgcccgag cgcgaacccg ctgcgcgcgc cgtagatgac gtgcggcgcc
     2941 atcgacatcg actccatccc gccgacgagc gccgtgcgcg cttcgcccag aaggatcatc
     3001 tgcgcgccgg agatgacgga ctggatgccg ctgccgcaga ggcggttgac ggtgagcgcg
     3061 ggccggtcgt gcggcacgcc cgccttcagg gcaacgtggc gcgcgccgta gatggcgtcg
     3121 cccgaggtct gcaacgcgtt gccgaagacg gtgtggtcga tctcttccgc cgccacgccc
     3181 gcagcttcga gcgcgccgcg cgcggcggcg gccccgaggt cgatggcctt cacgtccttc
     3241 agctcgccga cgtacgaaga catcggcgtg cgcttgccgc ccagtatgaa aatgtcttga
     3301 gccatgattt acgacacgtc ctctctatga aaaatccgac gtttggatga accggaaagt
     3361 ttaacggagg cccggaggcg tcgtcaattt agggcgccgt tcagtctgtg aagcggtgcg
     3421 cggcgcggta gcggtggagg agttcttcga ggaagccttc gtaggtgaag gtgtcggggt
     3481 cgaaggcggg gcggattttg tagaggatgg gggtggcgat gcgccaggcc atccactggc
     3541 gcggcgcttc cagcaagtcc cagcggcggc ggaggaaggc ttcgaccacc tcgatctccg
     3601 gctcggtgag cggggccagc gaggccgaga aggggacggg cttgaaggag cgccggagcg
     3661 ccgtgtcgct cgtcggcgcc gagaagacct cctcgaagga gggcgcctgc gcctcgcgct
     3721 cgcggacgac cacggtgccc gcgaccatgt cgccgatgcg ctggtcgcgg cggctggcga
     3781 agaccgcgac cagcccgatg gagtagaacg gcacgccgag ccacggcatc atgtcgaaga
     3841 tgcgcagcag gttgcgcgcc gccgcctccc agaacgtgac gggccgcccg tcctcgcgga
     3901 tcacgcgcag cttgagccaa cgcttgcccg gcgtctgccc gcgccacgcc cactcgaaca
     3961 gcacgaagta gctcgaccag acgaggaaga ccgcgaccag catcagcgcc accacccact
     4021 tcggcgcgtt cttcaagtcc tcgtaataac tggccgcgtt ccccagccac acgaagaaga
     4081 agacgaccag cgcgatggcg acgacctgca acgtgtggtc gaaggcgcac gccaggaagc
     4141 ggttgccgat ggaggccagc gcgaagtgca gcggcacgcg ctccggcgtc tcgatcacca
     4201 gcgtctcgtc tgtcggggct gcggctgcga ctgacataaa gtgatgcgga cggccacggc
     4261 gacctgcgtc ctgcgtcaac gccgccgccg gtcggacaaa agtttagcgc tgacggggcc
     4321 gacccgccgc gcctttcatc tcttcggcgg gagcatctgc gcttcccacc tcgcgtattc
     4381 ggcgcgcagg gcttcgaact cggcccggcg tgacgctttg aagtcggcct gctcgcgttc
     4441 atcgaccgag aggtcgaaga ggaactcgct cttgtcgtcg gggcggtaat acttccagcg
     4501 ccccctgcgc gccgccgact gcttgttgtg gcgccagaag agcgcgcgct cgaagggggc
     4561 gcgcgtgccc ctcaggaccg gcagcaggtc ttcgccgtcg agcgggtatg cggggtctgc
     4621 cttcgcgccc gcggcggcga ggatggtcgc cgtccagtcc atcgtgacgg cgacctggcg
     4681 gctgacccgg ttggcgggca cgacccccgg ccagcgcacg atggcgggca cgcgcaggcc
     4741 cccttcgagc agctcgaact tcttgccgga gaagggccag ttgaaggaga accgctcgcc
     4801 gccgttgtcg ctcgtgaaga cgacgagcgt gtcgcgttcg agccgcgccc ccttcagcgc
     4861 ccgcaacacc ctgccgacgc cgtcgtccag actcttcatc atcgcggcgt aggcttgcag
     4921 cgagccgccc gcggtcatcc ccgcgtagcc gagcgcgccg gacttggcgc ccgcggcatc
     4981 gcccggcccc tcccagggcc agtgcggcgc cgtgtagtgg agcgagaggt agaacgggcg
     5041 ctcttcgccg cgccgccgcc cgaggtactc gacggcgcgc tcggtcaaaa ggtcggtcat
     5101 gtagcccgcg cgctcgaccg gcacctcgcc ctcgaagaag tccggctcgt cgttcgcgtc
     5161 cttgtgcgtg aagtaggtcg cgccgccgct catgatgccg aagaactctt cgaagccgct
     5221 cttgacgggg ccgtaggtcg gcaggtagcc gaggtgccac ttgccgacga gcgccgtccg
     5281 gtagcccgcc gccttcagca acgacgcgac ggtcgggtgt tcgggcggga gaccgacatc
     5341 acgcttctgc tgttcgagct gcttccgcca cgacagcggc tctt
//
LOCUS       DBNGOH010001014         3592 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1804593, whole genome shotgun sequence.
ACCESSION   DBNGOH010001014 DBNGOH010000000
VERSION     DBNGOH010001014.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3592)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3592)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3592
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1804593"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1023
                     /locus_tag="AC159P_19730"
     CDS             <1..1023
                     /locus_tag="AC159P_19730"
                     /EC_number="6.6.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF014563.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobaltochelatase subunit CobN"
                     /protein_id="HIR3697727.1"
                     /translation="NPVQTGMQVAIPEIDGATEPFVYGGMTAEGPEPVALEERCARIA
                     RRLRRWNKLRTDDAADIRIALVLFCFPPNKGNIGTAAELDVFESVRAMLERLQSEGYR
                     TEVPADADELRERLLGGNSESFGAVANVAHRMSADEYLRLCPYAKEIEAEWGRAPGSI
                     NSFGRDLLIHGLRLGNVFLAVQPTFGYEGDPMRLLMARGGAPHHGFAALYTYLEKIFA
                     ADAVVHVGTHGALEFMPGKQVGLSGACWPDRLIGELPHVYVYSVNNPSEGTIAKRRSY
                     AELVSYFTPPVEDAGLYSELAALKEQLNAYRHATDERERERLFDEIEEKAASLHFLSD
                     EEAQAV"
     gene            1235..3559
                     /locus_tag="AC159P_19735"
     CDS             1235..3559
                     /locus_tag="AC159P_19735"
                     /inference="COORDINATES: protein motif:HMM:NF019607.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HIR3697728.1"
                     /translation="MKRRVLASSILLVVVLALAAGKVYDSRASARQTSELPVIALVAP
                     ITGLSVPVGITHAGDGSGRLFIIEQAGRIRVFKNGALLATPFLDISARVIDGFGERGL
                     LGLAFPHDYATKGYFYVNYIDNSNNTVVARYRRGASDPDIADPASEQIVITVEQPAFP
                     NHKGGHLAFGPRDRQLYVGLGDGGSGGDPDNRAQNPLQLLGKLLRLDVETGRPYRYTV
                     PSDNPFATHAGFRPEIWALGLRNPWRYSLDRATGDLYTADVGQGLWEEVNFQPAASAG
                     GENYGWRIMEGLHCFNPSSGCNMTGLTLPVHEYSHAGGNCSVTGGYVYRGATFARMQG
                     LYIYGDYCTGIIWGLRRDGTGAWQNAQLLDTSINISTFGEDEAGNLYVASHNTGTVFR
                     IDDTGALPPPPPTPEPARVQFVSASQTVAEEGASATFNVSRSGDPSVALTVDYATSEA
                     TANERGDYTTARGTLAFAPGELTKTVSVFVTDDALDEPDETLGLTLQNVTGKASLGAP
                     SAATLTINDNDPPTAGANPIDDSAFFVRQHYADFLNREPDEGGLRFWTDNIESCGANQ
                     QCREVRRIDTSAAFFLSIEFQETGVLVYQLHKAATGNLPRFNDFMEDTQQAGRDVVVG
                     VGAWQAQLEANKRALVNEFVTRPSFFAQYPTGQTPAQYVDALNTNTGGVLTAAERDAL
                     VNGLTAGAETRATALRKIAENAEFRRVEFNRAFVLMQYFGYLRRNPNAAPDTNFDGYD
                     FWLGKLDQFDGNFVEAEMVRAFIESIEYRQRFGQ"
BASE COUNT          636 a         1303 c         1117 g          536 t
ORIGIN      
        1 aaccccgtgc agacggggat gcaggtcgcc atccccgaga ttgacggcgc gaccgagccg
       61 ttcgtctacg gcgggatgac ggctgaaggg ccggagcctg tcgcgctcga agagcgttgc
      121 gcgcgcatcg cgcggaggct gcggcgctgg aacaagctgc ggactgacga cgcagcagac
      181 ataagaatcg cgctcgtcct cttctgcttc ccgccgaaca aggggaacat cggcaccgcc
      241 gccgagctcg atgtgttcga gagcgtgcgg gcgatgctcg aaaggctcca aagcgaaggc
      301 tacaggacgg aagtcccggc ggacgcggac gagctgcgcg agcggctgct cggcggcaac
      361 tcggaaagct tcggcgcggt cgccaacgtc gcgcaccgga tgagcgcgga cgagtacctg
      421 cggctctgcc cttacgcgaa ggagatcgaa gccgagtggg gccgcgcgcc cggctcgatc
      481 aactccttcg ggcgcgacct cttgattcac ggcctcaggc tcggcaacgt cttcctcgcc
      541 gtccagccga ccttcggcta cgaaggcgac cccatgcgct tgttgatggc gcgcggcggc
      601 gcgccgcacc acggcttcgc ggcgctctac acgtacctcg aaaaaatctt cgcggcggac
      661 gccgtcgtcc acgtcggcac gcacggtgcg ctcgaattca tgccgggcaa gcaggtcggc
      721 ctctccggcg cgtgctggcc cgaccgcctc atcggcgagc tgccgcacgt ctacgtctac
      781 agcgtcaaca acccctccga gggcaccatc gccaagcgcc gctcctacgc cgagctggtc
      841 tcctacttca cgccgcccgt cgaagacgcg ggcctttaca gcgagctggc cgcgctcaag
      901 gagcagctca acgcctaccg ccacgcgacc gacgagcgcg agcgcgaacg cctcttcgac
      961 gagatcgaag agaaggcggc gtcgctccat ttcctgtcgg acgaagaagc acaggccgtc
     1021 tgaaagtcgt cacaaccgtc ggtgaatttg taggggcagg gcttgtcctg cccctacaac
     1081 tatctcgcac gcctcgacgc gcgccggaac ctggtctgaa cgtacctgca taaactttct
     1141 cccccagcgg cgaatgagtt tataatgccc gcgaccccga actccccacc acaatccgaa
     1201 cgcagagggc tttcgagaaa gggaggcttg gtgtatgaag cgcagggtgc tcgcgtcgtc
     1261 gattctgctc gtcgtcgtac tggcgctggc cgccggtaag gtttacgact cgcgcgcttc
     1321 ggctcgacag acttcggagc ttccggtcat cgcgctcgtc gcgccgatca cggggctgtc
     1381 ggtgcccgtc ggcatcacgc acgcgggcga cgggtcgggc cgcctcttca tcatcgagca
     1441 ggcgggccgc atccgcgtct tcaagaacgg cgcgctgctc gcgacgccct tcctcgacat
     1501 ctccgcgcgc gtcatcgacg gcttcggcga gcggggcttg ctcggcctcg ccttcccgca
     1561 cgactacgcg acgaagggct acttttacgt caactacatc gacaactcga acaacaccgt
     1621 cgtcgcgcgc tacaggcgcg gcgcttcgga cccggacatc gcagaccccg cgagcgagca
     1681 gatcgtcatc accgtcgagc agcctgcgtt ccccaaccac aagggcgggc atctcgcctt
     1741 cggcccgcgc gaccggcagc tctacgtcgg cctcggcgac ggcggcagcg gcggcgaccc
     1801 cgacaaccga gcgcagaacc cgcttcaact cctcggcaag ctgctccgcc tcgacgtgga
     1861 gaccggcagg ccctaccgct acaccgtccc ctctgacaac ccgttcgcca ctcacgccgg
     1921 cttccgcccc gaaatctggg cgctcggcct gcgcaacccc tggcgctact cgctcgaccg
     1981 cgcgaccggc gacctctaca cggcggacgt ggggcagggc ctgtgggagg aggtgaactt
     2041 ccaacccgcc gcgagcgcgg gcggcgagaa ctacggctgg cgcatcatgg aggggctgca
     2101 ctgcttcaac ccttcgagcg gctgcaacat gacggggctg accttgcccg tccacgagta
     2161 cagccacgcc ggaggcaact gttccgtcac gggcggctac gtctaccgcg gcgcgacctt
     2221 cgcgcgcatg cagggcctct acatctacgg cgactactgc acgggcatca tctggggttt
     2281 acgacgagac ggcaccggcg cgtggcagaa cgcgcaactg ctcgacacct cgatcaacat
     2341 ctcgaccttc ggcgaggacg aggcgggcaa cctctacgtc gccagccaca acacgggcac
     2401 ggtcttccgc atcgacgaca caggcgccct gccgccgcca ccgccgacgc ccgaacccgc
     2461 gcgcgtccag ttcgtcagcg cctcgcagac cgtcgccgag gagggcgcga gcgccacgtt
     2521 caacgtctcg cgttccggcg acccttcggt cgctctgacg gttgactacg cgacctcgga
     2581 ggcgacggcc aacgagcggg gcgactacac gaccgcgcgc ggcacgctcg ccttcgcgcc
     2641 cggcgagttg acgaagaccg tcagcgtgtt cgtcaccgac gacgcgctcg acgagccgga
     2701 cgagacgctc ggcctcacgc tgcaaaacgt caccggcaaa gcctcgctcg gcgccccgtc
     2761 cgccgcgacg ctcaccatca acgacaacga cccgccgacc gccggcgcca acccgatcga
     2821 cgattccgcc ttcttcgtcc gccagcacta cgcggacttc ctcaaccgcg agccggacga
     2881 gggcggcctc cgtttctgga cggacaacat cgagtcgtgc ggggcgaacc agcagtgccg
     2941 cgaagtccgc cgcatcgaca cgagcgccgc cttcttcctc tcgatcgagt ttcaggagac
     3001 cggcgtgctc gtctaccaac tccacaaggc ggcgacgggc aacctgccgc gcttcaacga
     3061 cttcatggag gacacgcagc aggcgggccg cgatgtcgtg gtcggcgtcg gcgcgtggca
     3121 ggcgcagctc gaagcgaaca agcgcgcgct cgtcaacgag ttcgtcacgc gcccttcgtt
     3181 cttcgcgcag tacccgaccg gccagacgcc cgcgcagtac gtcgatgcgc tcaacaccaa
     3241 caccggcgga gtcctcaccg cggcggagcg cgacgcgctc gtcaacggcc ttaccgcggg
     3301 ggcggagacg cgcgccaccg cgctccgcaa aatcgccgag aacgcggagt tccggcgcgt
     3361 cgaattcaac cgcgccttcg tgctgatgca atacttcggg tatctacgcc gcaacccgaa
     3421 cgccgcgccc gacacgaact tcgacggcta cgacttctgg ctcgggaagc tcgaccagtt
     3481 cgacggcaac ttcgtcgagg ccgagatggt cagggccttc atcgagtcca tcgagtaccg
     3541 ccagcgcttc gggcagtaag agcgtccgcg aggcggtctt atcggaacac cg
//
LOCUS       DBNGOH010001015         2368 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4918425, whole genome shotgun sequence.
ACCESSION   DBNGOH010001015 DBNGOH010000000
VERSION     DBNGOH010001015.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2368)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2368)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2368
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4918425"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..494
                     /locus_tag="AC159P_19740"
     CDS             <1..494
                     /locus_tag="AC159P_19740"
                     /inference="COORDINATES: protein motif:HMM:NF025046.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4149 domain-containing protein"
                     /protein_id="HIR3697729.1"
                     /translation="GWLKLLTAVAANVRLLLVALWLGAAVFFSFAVAPSAFAVLPSRE
                     LAGAMVTRTLAVVNVGGFIVALFGLLTMPLAVRAAGRFTMWAQSASLVVLAAVTAVGH
                     WVIAARLAEMRAAMRGTIDALAQNDPARVAFNALHGYSVAALLVGMLAALAAFFVMAR
                     RRQ"
     gene            637..1839
                     /locus_tag="AC159P_19745"
     CDS             637..1839
                     /locus_tag="AC159P_19745"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016628 - oxidoreductase activity, acting
                     on the CH-CH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BaiN/RdsA family NAD(P)/FAD-dependent
                     oxidoreductase"
                     /protein_id="HIR3697730.1"
                     /translation="MTTQTADVIVIGAGAAGLFCAAEAARRGRRVVVLEHNQRPGRKI
                     RISGGGRCNFTNVHSTPANFISANPHFAKSALARYTPADFVRLVERHRIRYHEKKLGQ
                     LFCDGSAQQITDMLLEECRRAGASVVCDCSVSEVARGESSNAETVRGAFNVRTNRGDF
                     GCSSLVVATGGLSIPPLGATDFGYRMARQFGLRLRATRPGLVPLTLRAAEAETFRSLS
                     GVSVDAVASCNGAEFRENVLVTHRGLSGPAILQVSSYWSPGEEIALDLVPGEDAAAWL
                     ASHRSREITLANLLAERLPRRFAHAWCELHGLVRPLRQYAPEELRRAAALLEGWRLRP
                     AGTEGYAKAEVTLGGVDTDELSSRTMEARKVPGLYFIGEVVDVTGHLGGHNFQWAWAS
                     GFAAGQAA"
     gene            1941..>2368
                     /locus_tag="AC159P_19750"
     CDS             1941..>2368
                     /locus_tag="AC159P_19750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697731.1"
                     /translation="MEILENIEEWRDRYARWKQINVIRDGDVGDNYPWVVNRHAPFTP
                     ARRALPMLNLALVTSAGAYIDGTEPFDTAVAGGDVTFREIPVEVEARDLKWAARGYDP
                     AGVLEDMNAQVPVTRLLEFEGNGIIGQLNPVWWSFCGFIP"
BASE COUNT          340 a          780 c          857 g          391 t
ORIGIN      
        1 agggctggct gaagctgctg accgcggtcg cggcgaacgt gcgcctgctg ctcgtggcgc
       61 tgtggctcgg cgcggcggtc ttcttcagct tcgcggtcgc gccgtcggcg ttcgccgtgc
      121 tgccgagccg cgagctggcc ggcgcgatgg tgacgcgcac gctcgcggtc gtcaacgtcg
      181 gtgggtttat cgtcgcgctg ttcgggctgc tgaccatgcc gctcgcggtg cgggcggcgg
      241 gacgcttcac gatgtgggcg cagtcggcct cgctcgtcgt gctcgcggcg gtgacggcgg
      301 tcggccactg ggtcatcgcc gcgcgccttg ccgagatgcg cgcggcgatg cgcggcacca
      361 tcgacgcgct cgcgcagaac gacccggcgc gcgtcgcctt caacgcactg cacggctact
      421 cggtcgcggc gctcctggtc gggatgctgg cggcgctggc ggcgttcttc gtgatggccc
      481 gccgcaggca gtaggcagcg ggcagcaggc agtaaaaaca aaagcagaga gagaggggct
      541 tcggactttc ttctcaagct tcttcttcat gccgacttcc gcttcctgct ttgactgcct
      601 actgcccgct gcctaccgcc tactgttttg tagttgttga cgacgcagac ggcggacgtg
      661 atcgtgatcg gcgcgggggc ggcggggctg ttctgcgcgg cggaggcggc gcggcgcggg
      721 cggcgcgtcg tcgtgctcga acacaaccag cggcccggaa ggaagatcag gatttccggc
      781 ggcgggcgct gcaacttcac gaacgttcat tccacgcccg ccaacttcat ctcggccaac
      841 ccgcacttcg ccaagtcggc gctcgcgcgc tacacgcccg ccgacttcgt ccgcctcgtc
      901 gagcggcacc gcatccgcta tcacgagaag aaactcggcc agctcttctg cgacggcagc
      961 gcacagcaga tcacggacat gttgctcgaa gagtgccgcc gcgcgggcgc gtccgtcgtc
     1021 tgcgattgca gtgtgagcga ggtcgcgcgc ggcgagtcgt cgaacgccga aaccgtccgc
     1081 ggtgctttca acgtcaggac gaatcgcggc gacttcggct gctcctcgct cgtcgtcgcc
     1141 acgggcggac tctccatccc gccgctcggc gcgacggact tcggctaccg catggcaagg
     1201 caattcggcc tgcgcctgcg cgcgacgagg ccggggctgg tgccgctcac gctgcgcgcg
     1261 gcggaggcgg agactttccg aagcttgagc ggcgtctcgg tcgatgcggt cgcgtcgtgc
     1321 aacggcgcgg agttccgcga gaacgtgctg gtgacgcaca ggggattgag cggcccggcc
     1381 atcctgcaag tctcttcgta ctggtcgccg ggcgaagaga tcgcgctcga cctcgtgccg
     1441 ggcgaggacg ccgccgcgtg gctcgcgtcg caccgcagcc gcgagatcac gctcgcgaac
     1501 ctgctggccg agcgtctgcc gcgcaggttc gcgcacgcgt ggtgcgagct gcacggcctt
     1561 gtgcgcccgc tcaggcagta cgcgcccgaa gagctgcggc gcgcggcggc gctgctcgaa
     1621 gggtggcgcc tgcggcccgc gggcaccgag ggttacgcga aggcagaggt cacgctcggc
     1681 ggcgtggaca cggacgagct gtcttcgcgg acgatggagg cgcgcaaggt gccgggcctc
     1741 tacttcatcg gcgaggtcgt ggacgtgacg ggccacctcg gcggccacaa cttccagtgg
     1801 gcgtgggctt ccgggttcgc ggcggggcag gcggcgtgaa cgaggcgtgc gattccaagc
     1861 ggttttgatt tagaatcagc gccgacgatt tcaaacacgc tacatctcat gatcggggtc
     1921 ggggccggag gaggcttcag gtggaaatct tggagaacat cgaggagtgg cgcgaccgct
     1981 acgcgcgctg gaagcagatc aacgtcatcc gcgatggcga cgtgggcgac aactacccgt
     2041 gggtcgtgaa ccggcacgcg cccttcacgc ccgcgcggcg cgcgctgccg atgctcaacc
     2101 tcgcgctcgt cacctcggcg ggcgcctaca tcgacggcac cgagccgttc gacaccgcgg
     2161 tcgcgggcgg cgacgtgacc ttccgcgaaa tccccgtcga ggtcgaggcg cgtgacctga
     2221 agtgggccgc gcgcggctac gacccggcgg gcgtcctgga agacatgaac gcgcaggtgc
     2281 ccgtgacaag gctgcttgag ttcgagggca acggcatcat cgggcagctc aacccggtgt
     2341 ggtggagctt ctgcggcttc atcccgag
//
LOCUS       DBNGOH010001016         2034 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4918780, whole genome shotgun sequence.
ACCESSION   DBNGOH010001016 DBNGOH010000000
VERSION     DBNGOH010001016.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2034)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4918780"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..826
                     /locus_tag="AC159P_19755"
     CDS             <1..826
                     /locus_tag="AC159P_19755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697732.1"
                     /translation="TAVAERINNSLSGYIWVPYAIEMDGQIIRLFDTHNHRPAPEEET
                     PTDYDRRWVLVERPLVIVGGELTLDDADLQWSEAARFYEAPFQMKSNGGTLVVDDFGR
                     QRVEPHDLLNRWIVPLEKRVDYLTLHTGKKIEVPFEQLVVFSTNLDEKDLVDEAFLRR
                     MGYRARVEPPTPSAYLEIFKRAAYTRGVKVEEASLDHVLGNYMRERRQMKACEPRDLL
                     DRMSDICLFEGRAPEVSVRLIDAAWCNYFGTSHGFGSQEAARKTTGELLDANPLQL"
     gene            864..1754
                     /locus_tag="AC159P_19760"
     CDS             864..1754
                     /locus_tag="AC159P_19760"
                     /inference="COORDINATES: protein motif:HMM:NF013618.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008933 - peptidoglycan lytic
                     transglycosylase activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HIR3697733.1"
                     /translation="MRSTLLVIVVLGLSATALADQLRMADGSVVVVDEAWEDAQGYWY
                     RRGGVTQFLERARVKGIERAPRADDAKKAAASDVAVVKTVADGVAQSAQPLETVWIHF
                     VGGAKMEVDEASESADGVWYKRGNVSIFIERARVEKVERERLVVEDEVAAAPGAKAKK
                     RERRWTTGSARLDSLIRQNGARFGVDPYLVFLVMEHESHFNAHAVSPVGARGLMQLMP
                     GTAARFGVRNSFDPAQNVMGGTRYLKELLARFNDRVDLVLAGYNAGEGAVMKYGRRVP
                     PYRETRNYVRRITAKYQGGE"
     gene            1775..>2034
                     /locus_tag="AC159P_19765"
     CDS             1775..>2034
                     /locus_tag="AC159P_19765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697734.1"
                     /translation="MKSDLRFRLTFFVATVAAALVSCWLLMGESSPFHDYFLYHVTLP
                     NVWRALNVMPLVLGLLISGNPHSASTASAIPLSSSLMTQPPQS"
BASE COUNT          342 a          694 c          696 g          302 t
ORIGIN      
        1 gaccgccgtc gccgagcgca tcaacaacag cctctcgggc tacatctggg tgccctacgc
       61 gatcgagatg gacgggcaga tcatccgcct cttcgacacg cacaaccacc ggcccgcgcc
      121 cgaagaggag acgcccacgg actacgaccg ccgctgggtg ctggtcgagc gcccgctcgt
      181 catcgtcggc ggcgagctga cgctggacga cgcggacctc cagtggagcg aggccgcgcg
      241 tttttacgag gcgccgttcc agatgaagtc gaacggcggc acgctcgtcg tggacgactt
      301 cgggcgccag cgcgtcgagc cgcacgacct gctgaaccgc tggatcgtgc cgctcgaaaa
      361 gcgcgtcgat tacctgacgc tccacacggg caagaagatc gaggtgccgt tcgagcagct
      421 cgtcgtcttc tcgaccaacc tcgacgagaa agacctcgtg gacgaagcct tcctgcgccg
      481 catgggctac cgcgcgcgcg tcgagccgcc gacgccctcg gcctaccttg agattttcaa
      541 gcgcgccgcc tacacgcgcg gcgtcaaggt ggaggaggcg agcctcgacc acgtgctcgg
      601 caactacatg cgcgagcggc ggcagatgaa ggcgtgcgag ccgcgcgacc tgctcgaccg
      661 catgtcggac atctgcctct tcgaggggcg tgcgccggaa gtcagcgtcc gcctgataga
      721 cgcggcgtgg tgcaactact tcggcacgtc gcacggcttc ggcagccagg aggccgcgcg
      781 caagaccaca ggggagttac tcgatgcgaa cccactccag ctctgaaggc cgcgcgcgaa
      841 acgtgcgggc gacgcggtcg ttgatgagaa gcacgctgct cgtcatcgtc gtgctcggcc
      901 tgtccgcgac ggcgctcgcc gatcaactgc ggatggccga cggctcggtc gtcgtcgtgg
      961 acgaggcgtg ggaggacgcg caaggctact ggtatcgccg cggcggcgtc acgcagttcc
     1021 tcgaacgcgc gcgcgtgaag ggcatcgagc gcgcgccgcg cgcggacgac gcgaagaagg
     1081 cggcggcgag cgacgtggcc gtggtgaaga cggtcgccga cggcgtggcg cagtcggcgc
     1141 agccgctgga gacggtctgg attcacttcg tcggcggcgc gaagatggag gtggacgagg
     1201 cgagcgagtc ggcggacggc gtctggtaca agcgcggcaa cgtctcgatc ttcatcgagc
     1261 gcgcgcgcgt cgagaaggtc gagcgggaac ggctcgtcgt cgaggacgag gtggccgccg
     1321 cgcccggcgc gaaggcgaag aagcgcgagc ggcgctggac gacggggagc gcgcggctcg
     1381 actcgctcat ccgccagaac ggcgcgcgct tcggcgtcga tccctacctc gtcttcctcg
     1441 tcatggagca cgagtcgcac ttcaacgcgc acgcggtctc gcccgtcggc gcgcgcggcc
     1501 tgatgcagct catgcccggc accgccgcgc gcttcggcgt gcgcaactcg ttcgacccgg
     1561 cgcagaacgt gatgggtggc acgcgctatc tcaaggagct gctcgcgcgc ttcaacgacc
     1621 gcgtcgatct cgtgctcgcg ggctacaacg cgggcgaggg cgccgtcatg aagtacggtc
     1681 gccgcgtccc cccctaccgc gagacgcgca actacgtccg ccgcatcacg gccaagtatc
     1741 agggcggcga gtgacgaatc aaggcccggc tcgtatgaaa agcgatttgc gattccgcct
     1801 gacgttcttc gtggcgacgg tcgccgccgc cctcgtctcc tgctggctgc tgatgggcga
     1861 gagttcgccc ttccacgact acttcctcta ccacgtcacc ctgccgaacg tgtggagggc
     1921 gctcaacgtg atgccgctcg tgctgggcct gctcatcagc ggcaacccgc acagcgcgag
     1981 cacggcgagc gcgatccctc tcagttcaag cctcatgaca caaccccccc agtc
//
LOCUS       DBNGOH010001017         3058 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3635740, whole genome shotgun sequence.
ACCESSION   DBNGOH010001017 DBNGOH010000000
VERSION     DBNGOH010001017.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3058)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3058)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3058
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3635740"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..740)
                     /locus_tag="AC159P_19770"
     CDS             complement(<1..740)
                     /locus_tag="AC159P_19770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009224138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 130 protein"
                     /protein_id="HIR3697735.1"
                     /translation="MERAEFDARLRRLMREHEELVTRKNALAGGGNGVFERYRNPVLT
                     AAHAPVFWRYDLSHETNPHLLERMGVNAAFNSGAIEFGGKILLAVRVEGADRKSFFAV
                     AESENGVDNFRFWDYPVLMPELEDPDTNVYDMRLVRHEDGWVYGLFCTERKDPHAPPG
                     DLSSAIAQCGIARTRDLLTWERLPDLKTRSPQQRNVVLHPELIGGRYAFYTRPQDDFI
                     EAGSGGGIGWGLSDGIEHASVEEETVIDP"
     gene            complement(721..1965)
                     /locus_tag="AC159P_19775"
     CDS             complement(721..1965)
                     /locus_tag="AC159P_19775"
                     /EC_number="5.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013915846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AGE family epimerase/isomerase"
                     /protein_id="HIR3697736.1"
                     /translation="MKLDDEKLDGLRRRVESEWRDRIAPFWLKHAPDDEHGGFRGWLA
                     NDLRVDERADKGVVLDSRILWTFSRAYALREEPAYLQMARRAHAYLAEHFIDREHGGV
                     YWTLDYRGRPADTRKKIYAQAFALYGLCEFYAATGEGSALDEAFGIFRLLEEAGRDRE
                     RDGYFETFERDWTLAREQRLSEVDQDEKKSMNTHLHVLEAYAALLRVTRDARVEERLR
                     ALLDIFLAHIVHPREFYLRMFFDETWACRSARRSFGHDIEASWLLCEAAEVLGDAGVS
                     RRVRSVAARMAESVYASGLDADGGLLYEADAGGAVIDGDKHWWVQAEAVVGFLNAYAL
                     GGGEHFLEAAVRVWEFVEHNILDREHGEWFWKVSREGAVSLDLPKLSRWKCPYHNGRM
                     CFEVSRRLREIEEDASHGACGV"
     gene            complement(2011..>3058)
                     /locus_tag="AC159P_19780"
     CDS             complement(2011..>3058)
                     /locus_tag="AC159P_19780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697737.1"
                     /translation="YPDPSKSMLADIERFHRRIGGRKVYVIGEFGFVPLGEVRKILDA
                     VIGKGISGAMTWSLRFHNRDGGFYWHSEPLGGGLYKAYHYPGFASGAAYEEAALLGLM
                     REKAFEIRGLAAPPVEAPAPPFMLPTKSVAEISWRGSAGAAGYDIERATKRGGPWTVV
                     GRDVDDTRVQYRPLFSDDTASEGTSYYYRARAKNAAGASAPSNVVGPLRAEGRTIIDE
                     LGDFSRTFARGGLVQLETANARPYKEDTHRLRGGAGGWVTYRTAGPLRSAAVLVFTEG
                     AGDDFELYLSRDGASFEKAEPKVSRFPTAVNPSGYKLPVRYELAAPAPGTRFLKIVFR
                     AEAQLSRVELRHGK"
BASE COUNT          483 a         1065 c          995 g          515 t
ORIGIN      
        1 gggtcgatga ccgtttcttc ctcgaccgaa gcgtgctcga tgccgtcgga cagaccccag
       61 ccgatgccgc cgcccgaccc ggcctcgatg aagtcgtcct gcgggcgcgt gtagaaggcg
      121 tacctgccgc cgattagttc ggggtgcagg acgacgttgc gctgctgcgg cgagcgcgtc
      181 ttcaaatccg gcaggcgctc ccacgtcagc aagtcgcgcg tgcgcgcgat gccgcactgc
      241 gcgatggccg acgacaggtc gccgggcggc gcgtgcgggt ctttgcgctc cgtgcagaag
      301 aggccgtaga cccatccgtc ctcgtgtcgc acgagccgca tgtcgtagac gttcgtgtcc
      361 gggtcctcca gttcgggcat caggacgggg tagtcccaaa agcggaagtt gtccacgccg
      421 ttctcgctct cggcaacggc gaagaaggat ttccggtcgg cgccttcgac cctcacggcc
      481 agcagaatct tgccgccgaa ctcgatggcg cccgagttga acgccgcgtt gacgcccatc
      541 cgctccaaca ggtgcgggtt cgtctcgtgg ctcaggtcgt agcgccagaa gacgggcgcg
      601 tgcgcggcgg tcagcacggg gttcctataa cgctcgaaga cgccgttgcc gccgccggcc
      661 aacgcgttct tcctcgtgac cagctcctcg tgctcccgca tcagccgccg caggcgggcg
      721 tcaaactccg cacgctccat gtgacgcatc ctcctcgatt tcacgcagcc gccggctgac
      781 ttcgaagcac atccgcccgt tgtgataagg gcacttccac cggctcagct tcggcaggtc
      841 aagcgacacg gccccttcgc gcgacacctt ccagaaccac tcgccgtgct cgcggtcgag
      901 gatgttgtgt tcgacgaact cccacacgcg caccgcggcc tccaaaaaat gttcgccgcc
      961 gcccagcgcg tacgcgttca ggaagccgac gacggcctcg gcctgcaccc accagtgctt
     1021 gtcgccgtcg atgacggcgc cgcccgcgtc tgcctcgtag agcaggccgc cgtccgcgtc
     1081 aaggccgctc gcgtaaacag actccgccat ccgcgcggcg acggagcgca cgcgccggga
     1141 cactcccgcg tcgccgagca cttcggcggc ttcgcacagc agccagctcg cctcgatgtc
     1201 gtggccgaaa gacctgcgcg cggaacggca cgcccacgtc tcgtcgaaga acatgcgcag
     1261 gtaaaactct cgcgggtgga cgatgtgcgc gaggaagatg tcgagcagcg cgcggagtct
     1321 ctcttcgacc cgcgcgtcgc gcgtgacgcg gaggagcgcc gcgtaagcct ccaggacgtg
     1381 gaggtgcgtg ttcatggact tcttctcgtc ctgatcgacc tcgctcagac gctgctcgcg
     1441 cgcgagcgtc cagtcgcgct cgaatgtctc gaagtagccg tccctctcgc ggtcgcggcc
     1501 agcctcttcg agcaggcgaa aaatcccgaa ggcttcgtcc agcgccgacc cctcgccggt
     1561 cgccgcgtaa aactcgcaca gcccgtagag ggcgaacgcc tgcgcgtaaa tcttcttcct
     1621 cgtgtccgca ggtcgcccgc ggtaatccag cgtccagtag acgccgccgt gctcgcggtc
     1681 gatgaagtgc tcggcgaggt acgcgtgggc gcgccgtgcc atctgtaggt aagcaggctc
     1741 ctctcgtagc gcgtaggcgc gcgagaaagt ccagaggatg cgcgagtcga ggacgactcc
     1801 tttgtcggcc cgctcgtcaa cgcgcaagtc gttggcgagc cagccgcgga aacctccgtg
     1861 ctcgtcgtcc ggcgcgtgct tcagccagaa gggggcgatg cggtcgcgcc attcgctttc
     1921 gacccgccgc cgcagcccgt ccagcttttc gtcatccagc ttcatctctc gtacgaggcc
     1981 gccgtgtcct tatcacgcag gccgacagtc tcacttgccg tgccgcagct cgacgcggct
     2041 gagttgagct tcggcgcgga aaacgatctt gagaaagcgc gtgccgggcg cgggggccgc
     2101 gagttcgtaa cgcaccggca gcttgtagcc ggaggggttg accgcggtgg ggaagcggct
     2161 gaccttcggc tccgctttct cgaagctcgc accatcgcgc gaaagataga gttcgaagtc
     2221 gtcgcccgcg ccttccgtga agaccagcac cgcggcggag cgcagcggcc ccgccgtgcg
     2281 gtacgtcacc caaccgcccg cgccgcctct caggcggtgc gtgtcctcct tgtatgggcg
     2341 cgcgttcgcc gtctcaagct ggacgaggcc gccgcgcgcg aaggtgcgcg agaagtcgcc
     2401 caactcgtcg atgatggtgc ggccttcggc gcgcaagggg ccgacgacgt tcgacggcgc
     2461 cgaggcgcca gccgcgttct tcgcgcgcgc gcggtagtaa tagcttgttc cctccgaggc
     2521 cgtgtcgtcg gagaagagcg gcctgtactg cacgcgcgtg tcgtccacgt ccctgccgac
     2581 gactgtccac gggccgcccc gcttcgtcgc acgctcgatg tcgtaacccg cggcccccgc
     2641 cgagccgcgc cacgaaatct ctgcgacgga tttcgtgggc agcatgaagg gcggcgctgg
     2701 cgcttcgacg ggcggggccg cgaggccgcg aatctcgaac gccttctcgc gcattagccc
     2761 gagcagcgcc gcctcttcgt aagccgcgcc cgaagcgaag ccggggtagt ggtaggcttt
     2821 gtagaggccg ccgcccagag gctcggagtg ccagtagaag ccgccgtcgc ggttgtggaa
     2881 gcgcaggctc cacgtcatcg cgcccgagat gcctttgccg atgacggcgt cgagaatctt
     2941 cctgacctcg cccaaaggga cgaagccgaa ctcgccgatg acgtagacct tcctgccgcc
     3001 gatccggcga tggaaccttt cgatgtcggc cagcatcgac ttcgacgggt ctgggtag
//
LOCUS       DBNGOH010001018         1879 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_154009, whole genome shotgun sequence.
ACCESSION   DBNGOH010001018 DBNGOH010000000
VERSION     DBNGOH010001018.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1879)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1879)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1879
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_154009"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(90..512)
                     /locus_tag="AC159P_19785"
     CDS             complement(90..512)
                     /locus_tag="AC159P_19785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697738.1"
                     /translation="MKSLNIKRLAVAALTLAALCASSMWATGTGAQTTGSSKFYRLVA
                     VTGAEGYDLQWTDGRVVGLRALREDGSWFDLRPQAPPEEGQTPRFECSGWNKELYCYE
                     DDENQMSVCVCVDYISIGPGPVVGPVVDSWEFVKRTSR"
     gene            complement(690..>1879)
                     /locus_tag="AC159P_19790"
     CDS             complement(690..>1879)
                     /locus_tag="AC159P_19790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697739.1"
                     /translation="VQPQWSPTGARIAYWGKHKAGQRDIWTVTAAGGPPVAVTDDAAF
                     DWNPVWSPDGRHIYFSSDRGGSMNLWRVPLDERTGRVNGQPESVTAPSRYAFHLSFSR
                     DGRRAVFVNQTSTTHIYKAAFNPQREAITAPPVAVTRGFKHTSTPDLSPDGEWFAYSS
                     QGETREDIYVIDKDGATAPRQLTDDEHKDRDPRWSPDGKSIVFYSNRTGKYEAWAVNT
                     DGSNPRQLTFTEGANVAYPFWSPDGSHLVFNRNDESSTVVELGRPPEAPPARRLPLIP
                     DRSLGAVWCSSWSPDGSRLGCWLGSRSEHPGIYLYTLATDSYERLTNFGVHPRWFEDN
                     RKLLFYHQGTLHMVNAETKKVTEILSPPPPYRVIGFGISKDNRTIFYGLASTDADVWL
                     INL"
BASE COUNT          287 a          580 c          665 g          347 t
ORIGIN      
        1 tgggatgtcg aactgcccct gcctgatctc ctgcaccagc tctatcccgc tgaagagtga
       61 ctgccgcgct tccgaaattc ttgaaggcgt cagcgcgaag tcctcttcac gaactcccat
      121 gagtcaacga ccggcccgac gaccggcccc ggccctatcg atatgtagtc aacgcacacg
      181 cacacgctca tctgattctc gtcgtcctca tagcagtaca gctccttatt ccagccggaa
      241 cactcgaagc ggggtgtctg gccctcttcg ggcggggcct gtggccgcag gtcgaaccaa
      301 ctgccgtcct cacgcagcgc ccgcagaccg acgactctgc cgtccgtcca ctgcaagtcg
      361 tagccctcgg cccccgtcac tgcgacgagg cgatagaact tgctactgcc cgtcgtctgg
      421 gcgcccgtcc cggtggccca catggacgag gcgcagagcg ccgccagggt cagcgcggcc
      481 acggccagcc gtttaatgtt caaggatttc atctcggtca tctccctttc cgtcagcaga
      541 tttattcgaa cacgccccct aatgcgggag agtcgggcga aagctatcag cagcggcgag
      601 atttttttcg agtggccggg tctcgggaaa gtgaggggtg cggcgcgccg cgggcgcggg
      661 ttaacccgcg cttgagacgc gcgagtccgt cagaggttga tgagccacac gtctgcgtcg
      721 gtcgaggcga ggccgtagaa gatcgtgcgg ttgtctttgg agatgccgaa gccgatgacg
      781 cggtacggcg gcggcggcga gaggatttcc gtcacctttt tcgtctcggc gttgaccatg
      841 tgcagcgtgc cctggtggta gaaaagcagc ttgcggttgt cttcgaacca gcgcgggtgg
      901 acgccgaagt tggtcaggcg ttcgtagctg tcggtggcta acgtgtagag gtagatgccg
      961 gggtgctcgg agcgcgaccc gagccagcag ccgagtcggc tcccgtcggg agaccacgaa
     1021 gagcaccaga cggcgccgag cgagcggtcc gggatgagcg gcaggcggcg cgcgggcggc
     1081 gcttccggcg gcctgccgag ttcgacgacg gtggacgact cgtcgttgcg gttgaagacg
     1141 aggtgcgacc cgtcgggcga ccagaagggg taggcgacgt tcgcgccctc ggtgaaggtc
     1201 aactgccgcg ggttgctgcc gtctgtgttc acggcccacg cctcgtactt ccccgtgcgg
     1261 ttcgagtaga agacgatgct cttcccgtcg ggcgaccagc gcgggtcacg atctttgtgt
     1321 tcgtcgtcgg tgagctgtcg cggcgcggtc gcgccgtctt tgtcgatgac gtagatgtcc
     1381 tcgcgcgtct cgccctgcga gctgtaggcg aaccactcgc cgtcgggcga gaggtcgggc
     1441 gtcgaggtgt gtttgaagcc gcgggtgacg gcgacgggcg gcgcggtgat cgcctcccgt
     1501 tgcgggttga aggcggcctt gtagatgtgc gtggtgctcg tctggttgac gaagacggcg
     1561 cgccgcccgt cgcgcgagaa gctcaggtgg aaggcgtagc gcgagggcgc ggtgacggat
     1621 tcgggctgtc cgttgacgcg gcccgtccgc tcgtccagag gcacgcgcca gaggttcatg
     1681 ctgccgccgc ggtcgctgga gaagtagatg tgtctgccgt cgggcgacca gacggggttc
     1741 cagtcgaagg ccgcgtcgtc ggtgacggcg acgggcgggc cgcccgccgc cgtcactgtc
     1801 cagatgtcgc gctggccggc cttgtgcttg ccccagtagg cgatgcgcgc gccggtcgga
     1861 gaccactgcg gctgcacgg
//
LOCUS       DBNGOH010001019         7207 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1620668, whole genome shotgun sequence.
ACCESSION   DBNGOH010001019 DBNGOH010000000
VERSION     DBNGOH010001019.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7207)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7207)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7207
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1620668"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..425
                     /locus_tag="AC159P_19795"
     CDS             <1..425
                     /locus_tag="AC159P_19795"
                     /inference="COORDINATES: protein motif:HMM:NF012720.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HIR3697740.1"
                     /translation="VGKSRKVQVGFMVATAILGLTFLGVKAVEYSTKYHDGLIPIQGM
                     FDPAQGGIHMPEGVTVQQYQLFYWIYFAMTGLHALHMIIGVAILLPIMWWAWRGRYSP
                     EYHAPVENFGLYWHFVDIIWIFLFPLLYLLGAHFSGGH"
     gene            556..894
                     /locus_tag="AC159P_19800"
     CDS             556..894
                     /locus_tag="AC159P_19800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HIR3697741.1"
                     /translation="MSEHIVPRSTYFAVFGALLVGTAITVFAATIDFGPMNDVIAMTI
                     AVTKAMLVLLFFMHVRYSSRLIWVVVSSMFFWLFILLALTLSDYRTREWFSPLKQGPG
                     RQDPAHISTH"
     gene            1206..2561
                     /locus_tag="AC159P_19805"
     CDS             1206..2561
                     /locus_tag="AC159P_19805"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013702.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006644 - phospholipid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HIR3697742.1"
                     /translation="MLIAVLRFALRVFFRRIEVVGKERVPRSGATVFVLNHPNGLVDP
                     VFVLCHAPRRVSFLAKSTLFKMPVIGYLVRAMEAIPVYRKQDAGEDTSKNRETFGRSA
                     ELLRRGGTLAICPEGKSHSEPSLQPLKTGAARIALGAASSGAALDLKIVPAGLYYTAK
                     TTFRSGALLYFGEPIPVPAVPLEEDGDPPREAVRELSDRVAAALRSLTLNADQHEALQ
                     MVARADRIFSSAEEDADERTSLERELRRRRRFVEGYAFHRENSSRRLEELVRRIRQYE
                     EELQQAGLEDPRDLSTATVSKHASLTHLVTRGLVFLLLAPLALAGAVLHYPAYTLAGY
                     LATKFSASYDDVLSTFKIIASLLLFPLTWLALAVVAYLLFGWWGVLGALTLAPVAGYT
                     ALRFSEEFDRFVAGARAVLFFVRQRRFFTRLLDERRAIRKEILELGYEAERAGALKTG
                     T"
     gene            complement(2563..3537)
                     /locus_tag="AC159P_19810"
     CDS             complement(2563..3537)
                     /locus_tag="AC159P_19810"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3697743.1"
                     /translation="MKRFRKVVALTLMTVALFHAAPAGRGQKNAGRVFADSEVYRAPE
                     PSPEARAEMERKLAEARAAYERNPRDADAVIWLGRRTAYLGRFNEAIAVFTEGVRRHP
                     RDARMYRHRGHRYITTRRFGEAVKDLKRAAKLVAERPDEVEPDGQPNARGVPTSTLNS
                     NIWYHLGLAYYVKGDFKSAARAYRECLKFSKNADMLAATSHWLYMTLRRLKRDEEARR
                     VLEGIRDDADVIENHDYQRLLLMYKGRLTPEALMEEADKKRDSVGFATVGYGVGNFYF
                     YTGRALEGFPIFSRIVNGPQRTSFGYIAAEAEQRRAGIHVNHVRIIQD"
     gene            complement(3566..4471)
                     /gene="proC"
                     /locus_tag="AC159P_19815"
     CDS             complement(3566..4471)
                     /gene="proC"
                     /locus_tag="AC159P_19815"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008481900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0055129 - L-proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HIR3697744.1"
                     /translation="MLTQAEAAGHADENPLRAAHLSFVGCGVMAEAMVAGLLRRRLVA
                     PAQITASHPRAARRDELAARYRIRAVESNRAAATAAHDRLDAAGGEGAAAAPSLVVLA
                     VKPQRVGGVLHDLKGAVAPTQLVLSIVAGARAETIAAELLHPSVVRAMPNTPAQIGQG
                     MTAWTATPQVTEAQLAQVRALLGALGREMYVENENMIDMATALSATGPTYIFMMMEAL
                     VDAGVHMGFSRHVAQELVTQTMLGSVLFARESHKHPAELRNMVTSPGGTSAEAIYQME
                     KGSLRTVLSKAVYAAYQRAVALGRR"
     gene            complement(4686..5495)
                     /locus_tag="AC159P_19820"
     CDS             complement(4686..5495)
                     /locus_tag="AC159P_19820"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3697745.1"
                     /translation="MRQFFAMRSLLVVLAACAYLAASLAGCGLRNRPADADANAGANG
                     NANDAAGAGADGEGAPQVAPLPEGSDARAHFDSGMEAYKANRDAEAVERFKEAVRLDP
                     EFAEAHYRLGLAYSAVGENDESDKAFEEAVRTYKAVVKREPKNGDAQYMLGISYGKLG
                     KYEDAVKVLKEAVKSDPEDDDKFYELGLAHSKLAQYREAVAAFNKALALNPDYFAATD
                     ALEKAKSGLERREAFLKQQEKLRKQKLGNLNANANSNANSNTPRPASTPPN"
     gene            5758..6600
                     /locus_tag="AC159P_19825"
     CDS             5758..6600
                     /locus_tag="AC159P_19825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cif family virulence factor"
                     /protein_id="HIR3697746.1"
                     /translation="MEQEPRATSVSPSEKTIPIDRDRTLVAPRFDEKSVQRARPAVPI
                     ARDAAGRFWSSSMLVACIVAGLIGGVAGGLGLALYQKRNAVGSPPPAVAGAPSTSSSP
                     VVAAVVDEAPPAAPAAASVEATAPASPSEVARAGEASAPAPENEGEAARGPGGTEEAE
                     LRGALDGWIAATNARDIGRQMDFYGETVGAFYLTRNVPREAVRAEKTRVLGSADAIDI
                     RAGRPAVRLSLDGQTATMRFRKKYSIAGGGNDRRGEVVQELRWRRTQDGWKIVSERDL
                     RVID"
     gene            6929..>7207
                     /locus_tag="AC159P_19830"
     CDS             6929..>7207
                     /locus_tag="AC159P_19830"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016876156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HIR3697747.1"
                     /translation="MIITTEYVDIDVEGGAMRTLLAAPKAEGEYPGVLFYSDIFQLTG
                     PMVRAAARLAGYGFVVAAPEIYHRIEPAGAVIPFDDAGRTRGLEDAART"
BASE COUNT         1087 a         2454 c         2414 g         1252 t
ORIGIN      
        1 aggtcggcaa aagccgcaag gtgcaggtcg gcttcatggt ggcgacggcc atcctcggtc
       61 tcaccttcct cggcgtcaag gcggtcgagt acagcacgaa gtaccacgac ggcctgatcc
      121 ccatccaggg catgttcgac cccgcacagg gcggcatcca catgccggag ggcgtcaccg
      181 tccagcaata ccagctcttc tactggattt acttcgccat gacgggcctc cacgcgcttc
      241 acatgatcat cggcgtcgcc atcctgctcc cgatcatgtg gtgggcgtgg cgcgggcgct
      301 actcgcccga atatcacgcg ccggtcgaga acttcggcct ctactggcac ttcgtcgata
      361 tcatctggat tttcctcttc ccgctgctct acctgctggg cgcgcacttc agcggcggac
      421 actgaaaggc cgtaaaccgt caaccgtgat gagtgaaaac cggtgacgag tgacgagcga
      481 tgagcgatga gggacgtgct gttctgactc atcacggttg acggtttacg gcttacggct
      541 attgggattg agcatatgtc tgagcacatc gtacccaggt caacctactt cgcggtcttc
      601 ggggcgctgc tggtcggcac ggcgatcacg gtcttcgcgg cgacgataga cttcgggccg
      661 atgaacgacg tgatcgcgat gaccatcgcc gtgacgaagg cgatgctcgt gctcctcttc
      721 ttcatgcacg tccgctacag ctcgcgcctc atctgggtgg tcgtctccag catgttcttt
      781 tggctcttca tcctgctcgc gctcaccctg agcgactacc gcacgcgcga gtggttcagc
      841 ccgctcaagc aggggccggg ccggcaagac ccggcgcaca tcagcacgca ctgacacgat
      901 tgcggaatgc ggattccgga tcgcggattg gaggaacgtt gacggttgat gacagcgctg
      961 ccttcatcaa tcgtcagccg tcatcactcg tcgtcgcttc acgaatccgc agtccgaaat
     1021 ccgcattccg caatcgccgc ctgtgtcgcc tcaagaaccg gcacatcccg cgccgtcgcc
     1081 cgaacgactt actgtaaaat gaacttacgg cgcggcggcg cggcggtgaa tccgccgcgg
     1141 cgcgggcgcg cgtaggacgg tcgggagggg ttttcgggaa gactttgaaa agactgacga
     1201 gacgcatgct gatcgccgtg ctgcgcttcg cgctgcgggt cttcttccgg cgcatcgagg
     1261 tcgtcgggaa agagcgcgtg ccccggagcg gcgcgaccgt cttcgtcctg aaccacccga
     1321 acgggctggt cgatccggtc ttcgtcctct gccacgcgcc gcgccgcgtc tcgttcctcg
     1381 ccaagtccac gctcttcaag atgccggtca tcggctatct ggtgcgcgcg atggaggcga
     1441 tccccgtcta ccgcaagcag gacgcgggcg aggacacttc gaagaaccgc gagacgttcg
     1501 ggcgctcggc ggagcttctg cggcgcggcg gcacgctcgc catctgcccc gagggcaagt
     1561 cgcactcgga gccttcgctc caaccgctga agacgggcgc ggcccgcatc gcgctcggcg
     1621 ccgcatcgag cggcgcggcg ctcgatctga agatcgtccc cgcaggtctc tactacaccg
     1681 ccaagaccac cttccgcagc ggcgcgctgc tctacttcgg cgagccgata cccgtccccg
     1741 ccgtcccgct cgaagaggac ggcgacccgc cgcgcgaggc cgtgcgcgaa ctctcggacc
     1801 gcgtggccgc ggccctgcgc tcgctcacgc tcaacgccga ccagcacgag gcgctccaga
     1861 tggtcgcgcg cgccgaccgc atcttctcgt ccgccgaaga ggacgcggac gagcggacga
     1921 gcctggagcg cgagctgcgc cgccgccgcc gcttcgtcga gggctacgcc ttccaccgcg
     1981 agaactcctc gcgccgcctc gaagagctcg tgcggcgcat ccgccagtac gaggaggagc
     2041 tgcaacaggc cggactcgaa gacccgcgcg acctttcgac cgccaccgtc tcgaagcacg
     2101 ccagcctcac ccacctcgtc acgcgcggcc tcgtcttcct cttgctcgcc ccgctcgcgc
     2161 tcgcgggcgc cgtcctccac tacccggcct acacgctcgc aggctacctc gccaccaagt
     2221 tctccgcgag ctacgacgac gtgctctcga ccttcaagat catcgcctcg ctgctgctct
     2281 tcccgctcac ctggctcgcc ctcgccgtcg tcgcgtatct attgttcggc tggtggggag
     2341 tgctcggcgc gctcacgctc gcgcccgtcg cgggctacac ggccctgcgc ttctccgaag
     2401 agttcgaccg cttcgtggcg ggcgcgcgcg ccgtcctctt cttcgtccgc cagcgccgct
     2461 tcttcacgcg gctgctcgac gagcgccgcg ccatccgcaa ggaaattctc gaactcggct
     2521 acgaggccga acgcgcgggc gcgctcaaaa ccggcacctg aatcaatcct gaatgatccg
     2581 cacgtggttg acgtggatgc ctgcccggcg ctgctcggct tcggcggcga tgtacccgaa
     2641 actcgtccgc tgcgggccgt tgacgatgcg ggagaagatg gggaaacctt ccagcgcccg
     2701 ccccgtgtag aaataaaagt tgccgacgcc gtagccgacg gtggcgaagc cgaccgagtc
     2761 acgcttcttg tccgcctctt ccatcagcgc ctcgggtgtg agccgcccct tgtacatcaa
     2821 gagcaaccgc tggtagtcgt ggttctcgat cacgtccgcg tcgtcgcgta tcccttcgag
     2881 cacgcgccgc gcctcttcgt cgcgtttcag ccgccgcagc gtcatgtaga gccagtgcga
     2941 ggtggcggcg agcatgtccg cgttcttcga aaacttcagg cactcgcggt aggcccgcgc
     3001 cgcgcttttg aaatcgccct tgacgtagta ggcgaggccg aggtgatacc agatgttcga
     3061 gttgagcgtg ctggtcggca cgccgcgcgc gttcggctgc ccgtccggct cgacctcgtc
     3121 gggcctttcg gcgacgagct tcgcggcccg cttcaggtct ttcaccgcct cgccgaagcg
     3181 gcgcgtcgtg atgtagcggt ggccgcggtg gcggtacatc cgcgcgtcgc gcgggtgtcg
     3241 cctgacgccc tcggtgaaga ccgcaatcgc ctcgttgaag cgaccgaggt acgcggtgcg
     3301 gcgaccgagc cagatgacgg cgtccgcgtc ccgcgggttc cgctcgtagg cggcgcgcgc
     3361 ctccgcgagc ttccgctcca tctccgcgcg cgcctcgggc gaaggctccg gcgcgcggta
     3421 gacctcgctg tcggcgaaga cgcggcccgc gtttttctgc ccgcggcccg cgggcgcggc
     3481 gtggaagagt gcgacggtca tcagcgtcag cgcgacgacc ttgcggaagc gtttcaagtc
     3541 tctgactcct cgtggtcggc gtccctcatc tgcgaccgag cgcgacggcg cgctggtagg
     3601 cggcgtagac ggctttggag aggacggtgc gtaaggagcc tttctccatc tggtagatgg
     3661 cctcggcgga ggtgcccccg ggcgaggtga ccatgttgcg cagctccgcc gggtgcttgt
     3721 gcgactcgcg ggcgaagagg acggagccga gcatggtctg cgtgaccagc tcctgcgcga
     3781 cgtggcgcga gaaacccatg tggacgcccg cgtccaccag cgcctccatc atcatgaaga
     3841 tgtaggtcgg ccccgtcgcc gagagcgctg tcgccatgtc gatcatgttc tcgttctcga
     3901 cgtacatctc gcgcccgagc gcgccgagca gcgcgcgcac ctgcgcgagc tgcgcctccg
     3961 tcacctgcgg cgtcgccgtc caggcggtca tcccctgccc gatctgcgcg ggcgtgttcg
     4021 gcatggcgcg cacgaccgag gggtgcaaaa gctcggcggc gatggtctcg gcacgcgcgc
     4081 ccgcgacgat ggagagcacg agctgcgtcg gcgcgaccgc gcccttcagg tcgtggagca
     4141 cgccgccgac gcgctgcggc ttgaccgcga gcacgacgag cgagggcgcg gcggcggcgc
     4201 cctcgccgcc cgccgcgtcc agacggtcgt gcgcggcggt ggccgcggcg cggttgctct
     4261 cgacggcgcg gatgcggtag cgcgcggcca gctcgtcgcg gcgcgcggcg cgcgggtggc
     4321 tcgcggtgat ctgcgcgggc gcgacgaggc ggcggcggag cagtcccgcc accatcgcct
     4381 cggccatcac gccgcagccg acgaacgaga ggtgcgccgc gcgcagaggg ttctcgtcgg
     4441 cgtgcccggc ggcctccgcc tgtgtcagca tgtccgtcat cttcagcccg tgtccccttc
     4501 agcgaggcat aacgtcaggg cgcctatcat agacaccgcg cgcccgcgcg tccacaagaa
     4561 gaatcgaagt gaggagcgtc gaggcgattt cggattagca gaagcgttga cggttgacga
     4621 gagcactgct gtcatcaatc gtcgttaccg cttcaatcca aaatccgaaa tgcctgagtg
     4681 tttgttcagt tgggaggggt cgaggccggg cgcggtgtgt tcgagttggc gttcgagttg
     4741 gcgttggcgt tgaggttgcc gagcttctgc ttgcgcagct tctcctgctg cttgaggaac
     4801 gcctcgcgcc gctccagccc ggacttggcc ttctccagcg cgtcggtcgc ggcgaagtag
     4861 tcggggttga gcgcgagcgc cttgttgaag gcggcgaccg cctcgcggta ctgtgcgagc
     4921 ttgctgtgcg cgaggcccag ctcgtagaac ttgtcgtcgt cctcggggtc gctcttcacc
     4981 gcctccttca agaccttgac cgcgtcctcg tacttgccga gcttgccgta cgagatgccg
     5041 agcatgtact gcgcgtcgcc gttcttcggc tcgcgcttga cgaccgcctt gtaagtcctg
     5101 accgcctcct cgaaagcctt gtccgactcg tcgttctcgc cgaccgccga gtaggcgagg
     5161 ccgaggcggt agtgcgcctc ggcgaactcg gggtcgagcc gcacggcctc cttgaagcgc
     5221 tcgaccgcct ccgcgtcgcg gttggccttg tacgcctcca tcccgctgtc gaagtgggcg
     5281 cgcgcgtcgc tcccctcggg cagcggcgcg acctgcggcg cgccctcgcc gtcggcgccc
     5341 gcgcccgccg cgtcgttcgc gttaccgttc gcgcccgcgt tcgcgtcggc atccgcgggg
     5401 cggttcctca gcccgcaacc ggcaagcgac gcggcgaggt acgcgcaggc ggcgagcacg
     5461 acgaggagtg aacgcatggc gaaaaattgt ctcatggagg gactgcttcg acagtaaact
     5521 ttctacgcca aaacggcgcg gctcgtcaac cgggccaagg gagacgggga gatggggaga
     5581 gggggagtga gggagtagag agtgggggat aatataagcg gcgctcagct tcctgttctt
     5641 atctcccttt ctccccgtct ccccgtctcc cgtctgcttt gtggactggc gattgctctt
     5701 cgggtctgat gcgcccgtgc gcgtgtaccg gttgcaatca gcctggggga gttggaaatg
     5761 gaacaagaac ctcgcgccac ctccgtaagc ccttcggaga aaacgattcc catcgaccgc
     5821 gacaggacgc tcgtcgcgcc gcgcttcgac gagaaatccg tacagcgcgc gcgccccgcc
     5881 gtgcccatcg cgcgggacgc ggcggggcgc ttctggtcgt cgtcgatgct cgtcgcatgc
     5941 atcgtcgcgg gcctcatcgg cggcgtggcg ggcggcctcg gcctcgcgct ctatcagaag
     6001 aggaacgcgg tcgggtcgcc gcccccggcg gtcgcgggcg cgccttcgac gtcttcgtcg
     6061 ccggtcgtcg ccgccgtggt tgacgaagca ccgcccgccg cgcccgccgc cgcgagcgtc
     6121 gaagcgacgg cgcccgcgtc cccgtccgaa gtcgcgcggg cgggcgaagc gtccgcgccg
     6181 gctccggaga acgagggcga ggcggcgcgc gggccgggcg gtacggagga ggcggaattg
     6241 cgcggcgcgc tcgacgggtg gatcgcggcg acgaacgcgc gcgacatcgg gcgacagatg
     6301 gacttctacg gcgagacggt cggcgccttc tacctgacgc gcaacgtgcc gcgcgaggcc
     6361 gtgcgcgcgg agaagacgcg cgtcttgggc agcgccgacg ccatcgacat ccgcgccgga
     6421 cggcctgccg tccgcctcag cctcgacggc cagaccgcca ccatgcgctt ccgcaaaaag
     6481 tattcgatcg cgggcggagg caatgaccgc cgcggcgagg tcgtgcagga gttgcgctgg
     6541 cgccgcacgc aggacggctg gaagatcgtc agcgagcgcg acctgcgcgt gatcgattga
     6601 ggcgacacgt cgagaggcag gattcaacgc ggagggcgcg aagaggcgcc gaggacgcag
     6661 agaacaacag agacgaattc gaaattttca agtttcgaat ccgtcttttc ttctctgcgt
     6721 cctcggcctc tcttcggtgt tctctgcgtc gaagcgagtg cgcgctacac tcgacgggaa
     6781 agcttcgaat cgcatcgatg cgcggcgctc aaggaaaagc ttccggcgcg gcgctcgttt
     6841 gcgttagagt gtgccgttct tcgatccccg aagagggcga cgggtagcag aggcgttgtg
     6901 gttgcgtccg gcgcccgcgc tgagagcgat gataataacg accgagtacg tggacatcga
     6961 cgttgaaggc ggcgcgatgc ggacgctgtt ggcggcgccg aaggccgagg gcgagtatcc
     7021 gggcgtgctc ttctactcgg acatcttcca gttgacgggg ccgatggtgc gcgcggcggc
     7081 gcggctggcg ggctacggct tcgtcgtcgc ggcgcccgaa atctaccacc gcatcgagcc
     7141 ggcgggcgcg gtcatcccgt tcgacgacgc gggccgcacg cgcggcctcg aagacgcggc
     7201 gcgcacc
//
LOCUS       DBNGOH010001020         2974 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1621211, whole genome shotgun sequence.
ACCESSION   DBNGOH010001020 DBNGOH010000000
VERSION     DBNGOH010001020.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2974)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2974)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2974
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1621211"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..984
                     /locus_tag="AC159P_19835"
     CDS             <1..984
                     /locus_tag="AC159P_19835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013707315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR3697748.1"
                     /translation="YADVDRDKALKQGVAVGDVYQTLQAYLGGLYLNQFNRFGRQWRV
                     FLQAEGDERRSEQDIDRYYVRNREGDMVPLSALVTTRRISGPEYTNRFNLYRAAQVIG
                     GAAPGYSSGQAMTALEEVAKEVLPPEIGYDWADLSYQERQASGGAITAFALSLLFVFL
                     ILAALYESWSLPFSVLLTVPIAVFGGFVGLLLRGFDLDVYAQIGLIVLIGLTAKNAIL
                     IVEFAKLELEKGRSLAEAALEGARLRRRALFMTSFAFILGCVPLWFAEGSGASARQIL
                     GTVVIVGMLADTFIASFLIPVSFYVVEKLSRGVSERLSGTGTPAPEPAAGD"
     gene            1131..1610
                     /locus_tag="AC159P_19840"
     CDS             1131..1610
                     /locus_tag="AC159P_19840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HIR3697749.1"
                     /translation="MKTIAQEDYTSSFFQLLRETFEGPPPDTGSAYLDKGGGLFQTLE
                     PLTAEAASASARPDAATIAAHCEHVRFYVMALYELMRGATGKIDWEQSWLVKTVTPAE
                     WDDLKENLRRAYATVAEHLRTVEHWGDEEIGDGMAILMHTAYHLGAIRQLARALGPA"
     gene            1677..2951
                     /locus_tag="AC159P_19845"
     CDS             1677..2951
                     /locus_tag="AC159P_19845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011691853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3697750.1"
                     /translation="MNKASRKVQRVYLTLLLFNTLSASLIWGINTLFLLDAGLSNTEA
                     FTANAFFTAGFVLFEIPTGVVADLRGRRVSYLLGVLTLVLSTLLYLFMWYVAAPFRAW
                     ALASMLLGLGFTFFSGAVEAWLVDALAHSGFKEKLESVLARGEIVEGAAMLTGSVIGG
                     LVAQATNLGVPYILRAVLLAVTFVVALTLMRDLGFKPARGKRPLEEIKTILAGSVRHG
                     LRNPPVRWVMLAAPFTGGVSIYAFYAMQPYLLELYGDEKAYGVAGLAAAIVAGAQIAG
                     GLLVPHIGRIFGRRTSVLLTCEVLSVAVLGVVGFVPNFWVVVTLLILWGLMFAAVMPV
                     RQAYLNALIPSEQRATVLSFDSVFGSTGGVVIQPVLGKAADAWGYPVSYALSAAFQAL
                     AVPFTWLARREKAAPDAISSTEGRLDKGRVAT"
BASE COUNT          459 a          935 c         1030 g          550 t
ORIGIN      
        1 tacgccgacg tggatcgcga caaggctttg aagcagggcg tggcggtggg cgacgtgtac
       61 cagaccttgc aagcctatct cggcgggctg tatctgaacc agttcaaccg cttcggtcgt
      121 caatggcgag tgtttctaca ggcggaaggc gacgagcggc gcagcgagca ggacatcgat
      181 cgatactacg tgcggaacag agagggcgac atggtgccgc tctcggcgct ggtcacgacg
      241 cgccgcatca gcggcccgga atacaccaac cgcttcaacc tctaccgcgc cgcacaggtc
      301 atcggcgggg ccgcgcccgg ctacagctcg gggcaggcca tgacggcgct cgaagaggtg
      361 gcgaaggaag tcctcccgcc cgagataggc tacgactggg ccgacctctc gtaccaggag
      421 cggcaggctt cgggcggggc gatcacggcg ttcgcattgt cgctgctctt cgtgttcctg
      481 atcctggcgg cactctacga gagctggtcg ctgccgttct cggtgctgct gaccgtcccc
      541 atcgccgtct tcggcggctt cgtcggtctg ctgctgcgcg gcttcgacct cgacgtgtac
      601 gcgcagatcg gcctcatcgt gctcatcggc ctgacggcca agaacgcgat cctcatcgtc
      661 gagttcgcca agctcgaact cgaaaagggg cggagtctgg cggaggcggc gctggaaggg
      721 gcgcgcctgc ggcggcgcgc actcttcatg acctcgttcg cgttcatctt gggctgcgtg
      781 ccgctgtggt tcgccgaagg ttcgggcgcg tcggcgcgac agatcctcgg caccgtcgtc
      841 atcgtcggca tgctggccga cacgttcatc gccagcttcc tcatccccgt gagtttctac
      901 gtcgtcgaga agctctcgcg cggcgtcagc gagcggctgt cgggaaccgg cacccccgcg
      961 ccggaaccgg cggcgggtga ttgatgttgg tcgagagggc tgacgcggga tgtgacgccg
     1021 ggggcgtgca tgttggaaaa gcgacgtagc aggctcgcct tcgatgcttg tcatctcgat
     1081 gttgtagagt agttcgggtg agagccgaga acacgatgtc aggagtcggc atgaaaacaa
     1141 tcgcgcagga agattacacg agcagtttct ttcagctcct cagagagacc ttcgaaggcc
     1201 cgccgccgga cacgggcagc gcctacctcg acaagggcgg gggactgttc cagacgcttg
     1261 agccgttgac ggcggaagcg gcctccgcgt cggcgcggcc cgacgccgcc accatcgccg
     1321 cccactgcga acacgtgcgg ttctacgtca tggcgctcta cgaattgatg cgcggggcga
     1381 cgggtaagat cgattgggag cagagctggc tggtgaagac ggtgacgccg gcggagtggg
     1441 atgacctcaa agagaatcta cgccgcgcgt acgcgaccgt cgccgagcat ctccgaacgg
     1501 tcgagcactg gggcgacgag gagatcggcg acgggatggc gatcctcatg cacacggcct
     1561 accacctggg agcgatccgg cagctcgcgc gggcgctcgg ccccgcatga acggacgcgc
     1621 cgcgtcggaa atcgtcatgc cgtgcgtgag gccgttccgg tagcctgcgt cgagcgatga
     1681 acaaagcttc ccgtaaagtc cagagggtct acctgacgct gttgctcttc aacacgctgt
     1741 cggcctctct gatctggggc atcaacacgc tgttcctgct cgacgcgggg ctgagcaaca
     1801 cggaggcgtt caccgcgaac gcgttcttca ccgccggctt cgtgctgttc gaaataccga
     1861 ccggcgtggt ggccgacctg cgcgggagga gggtgtcgta cctgctcggc gtgctgacgc
     1921 tggtgctctc caccttgctc tacctgttca tgtggtatgt ggccgcaccc ttccgggcgt
     1981 gggcgctcgc ctcgatgctg ctcggtctcg gcttcacgtt tttctcggga gccgtcgagg
     2041 cgtggctggt ggacgcgctc gcgcacagcg gcttcaagga gaagttggag tcggtgctgg
     2101 cgagaggcga gatcgtcgag ggcgccgcga tgctcaccgg gtctgtcatc ggcgggctgg
     2161 tcgcgcaagc gaccaacctc ggagtcccct acatcctccg cgcggtgctg ctcgcggtca
     2221 cgttcgtcgt cgccctgacg ttgatgagag acctcggctt caagccggcg cggggcaaac
     2281 gtcctctcga agagataaag accattctgg ccggctccgt tcgccacggc ctgcgcaacc
     2341 cgcccgtcag gtgggtgatg ctggccgccc cgttcaccgg cggggtgtcg atctacgcgt
     2401 tctatgccat gcagccttac ctgctggagc tgtacggcga cgagaaagcc tacggcgtgg
     2461 ccggtctggc ggcggcgatt gtcgccgggg cgcagatcgc cggcgggttg ctcgtcccgc
     2521 acatcgggcg catcttcggt cggcggactt cggtgctcct gacgtgcgag gtgctgagcg
     2581 tcgcggtgct gggcgtcgtg gggttcgtgc cgaacttctg ggtcgtcgtc accctgttga
     2641 ttctgtgggg cctcatgttc gcggcggtca tgccggtgcg ccaggcgtat ctcaacgccc
     2701 tgatcccttc ggagcagcgc gccaccgtgt tgtcgttcga ttcggtgttc ggctcgaccg
     2761 gaggggtcgt catccagccc gtcctcggca aggccgcgga cgcgtggggc tacccggtct
     2821 cctacgcctt gagcgcggcc ttccaggcgc tggccgtccc cttcacatgg ctggcccgcc
     2881 gggaaaaggc cgcgccggac gccatcagct cgacggaggg gcggctcgat aaaggaagag
     2941 tcgcgacgtg agcagggcgg caggcccggc gctt
//
LOCUS       DBNGOH010001021         2750 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1073430, whole genome shotgun sequence.
ACCESSION   DBNGOH010001021 DBNGOH010000000
VERSION     DBNGOH010001021.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2750)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2750)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2750
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1073430"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="AC159P_19850"
     CDS             <1..201
                     /locus_tag="AC159P_19850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697751.1"
                     /translation="VGVGVGLAGAFVLTRLLRGLLFGVSVTDPLVFLGVAAVLSLAAL
                     AACLVPARRATKVDPMVALRYE"
     gene            362..1717
                     /locus_tag="AC159P_19855"
     CDS             362..1717
                     /locus_tag="AC159P_19855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HIR3697752.1"
                     /translation="MPRILIADDQADVLEALRLLLKGEGYEAESVSSPAAVLRALEAR
                     DFDAALIDLNYARDTTSGQEGLDLLSRIQSADNSLPVIVMTAWGSVDMAVEAMRRGAR
                     DFIQKPWDNARLMTVLRTQVELRQALRRGQRFEAENRVLRDGREVPALVAESEAMRRV
                     LDVVTRIAPADANVLITGENGTGKEVVARTLHALSARAGRPVVTVNTGALSEGLFESE
                     LFGHVKGAFTDARADRIGRFELADGGTLFLDEIADAHPALQAKLLRVLETGEFERVGS
                     SKTRRVNVRVLSATNADPHAEVAAGRFRQDLLFRLNTVEIHLPPLRERGQDILLLAEH
                     FLRQSARRYRRDITSFDKAARQALVEYSWPGNVRELNHVVERAVLLARAETVTAQDLA
                     LRGGAAGAPARLEEMSIEEVERLLIQKALARYQGNSNRAAEALGLSRSALYRRLQKYG
                     L"
     gene            1707..2102
                     /locus_tag="AC159P_19860"
     CDS             1707..2102
                     /locus_tag="AC159P_19860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697753.1"
                     /translation="MDSDTVLRAGTRAAPEATEQSRLFDLLGRINYVVLRGSGLYLQA
                     GGKVYRVRYGAYWQSVYSGEQHWELRCEEVGYGVWADGHETTFVIDLPIRYTRHGWVI
                     EVGSLFCLLYSDLLAVNGGAFEDAKLCRQ"
     gene            2189..>2750
                     /locus_tag="AC159P_19865"
     CDS             2189..>2750
                     /locus_tag="AC159P_19865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014393115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR3697754.1"
                     /translation="MRKEADTTESPRRAVRPAPPRARGERLNHENRITLMALAAGLPG
                     VLLSMLWLLGGDYTPKVKWTLGALVLGCWFGFALALRGRVVRPLQTVSNLLAALREGD
                     YSIRARGASYRDALGEVVVEVNALGETLREQRLGALEAAALLQTVMAEIEAAVFAFDA
                     GGRLRLVNRAGERILAQPAARLLGRRA"
BASE COUNT          395 a          921 c          999 g          435 t
ORIGIN      
        1 gtcggcgtcg gagtcggtct cgcgggggcc ttcgtgctga cgcgtttgct gcgagggctg
       61 ctgttcggcg tctcggtcac cgacccgctc gtcttcctcg gggtggcggc ggtgctctcg
      121 ctggcggcgc tcgcggcctg cctcgtcccc gcgcgccgcg cgacgaaggt agatccgatg
      181 gtggcgctcc gctacgagta gggaagttaa caccgcggac gcgccccgaa gagtcgggcg
      241 cgcggatcac gaccgccggg gcagaagccc ttgagcctcc ctgtgtcgtg acacgtgcgc
      301 gcgtttagga gtcctccgtt gtcgagtata agataggggg gcatctgccg ctttcttgcc
      361 tatgccgcga atcctgatcg ccgatgacca agccgacgtg ctggaggcgc tgcgcctgct
      421 gctcaaggga gagggttacg aggccgagtc ggtctcgtcg cccgccgccg ttctcagggc
      481 gcttgaggcg cgcgacttcg acgccgccct gatcgacctc aactacgcgc gcgacaccac
      541 gtcgggtcag gagggcctcg acctgctgtc gcgcatccag tccgccgaca actcgctgcc
      601 cgtcatcgtg atgacggcgt ggggcagcgt tgacatggcg gtcgaggcga tgcggcgcgg
      661 cgcgcgcgac ttcatccaga agccgtggga caacgcgcgc ctcatgacgg tgttgcggac
      721 gcaggtcgag ttgcggcagg cgctccggcg cgggcagcgg ttcgaagccg agaaccgcgt
      781 gctgcgcgac gggcgcgagg tgccggcgct cgtcgccgag tccgaggcga tgcggcgcgt
      841 gctcgatgtc gtgacgcgca tcgcgcctgc cgacgccaac gttctgatca caggcgagaa
      901 cggcacgggc aaggaggtgg tcgcgcgcac gctgcacgcc ctctcggcgc gcgcgggccg
      961 ccccgtcgtg acggtcaaca cgggggcctt gagcgagggg cttttcgaga gcgagctgtt
     1021 cggccacgtc aagggcgctt tcacggacgc gcgggccgac cgtatcggac gcttcgagct
     1081 ggccgacggc ggcacgctct ttctcgacga aatcgccgac gcgcacccgg cgctccaggc
     1141 caagctgctg cgcgtgctgg agacgggaga gttcgagcgc gtcggctctt cgaagacgcg
     1201 ccgcgtcaac gtgcgcgtgc tctcggccac caacgccgac ccgcacgcgg aggtcgccgc
     1261 cggtcgcttc cgccaggact tgctcttccg cctcaacacg gtcgagattc acctcccgcc
     1321 tctgcgcgaa cgcggtcagg acatcctgct gctggccgag cacttcctgc ggcagtcggc
     1381 ccggcgctac cggcgggaca tcacctcgtt cgacaaggcg gcccggcagg cgctcgtgga
     1441 atactcctgg cccggcaacg tgcgcgagct gaaccacgtg gtcgagcgcg cggtgctgct
     1501 cgcgcgcgcc gagaccgtga cggcgcaaga cctcgcgctg cggggcgggg ccgcaggcgc
     1561 cccggcccgg ctcgaagaga tgagcatcga agaggtggag cgcctgctca tccagaaggc
     1621 gctggcgcgt taccagggga actcgaaccg cgccgccgag gctttgggcc tgagccgcag
     1681 cgccctctac cgtcgtctgc aaaagtatgg actctgacac cgtactgcgg gcaggcacac
     1741 gggccgctcc cgaagcgacc gagcagagcc gtctcttcga cctgctcggc aggatcaact
     1801 acgtcgtcct gcggggcagc gggttgtatt tgcaggcggg cggcaaggtc tacagggttc
     1861 gctacggggc ttactggcaa agcgtctact cgggcgagca gcactgggag ctgaggtgtg
     1921 aggaggtggg gtacggcgtc tgggccgacg ggcacgaaac gaccttcgtc atcgatttgc
     1981 ctatccgcta cacgaggcac ggctgggtca tcgaggtggg gtcgctcttc tgcctcctct
     2041 acagcgatct tctcgccgtc aacggcggcg ccttcgagga cgcgaaactc tgccgccagt
     2101 gaactccgat tcgatccgga ctgtcggcag ggggcggaag cggcccgtgc ccgttccgcc
     2161 ggtcggcgaa gggctatatc atacggcggt gcgaaaagag gcggacacta cggagtcacc
     2221 tcgccgcgcc gtgcgccccg cgccgccgcg cgcgcgcgga gagcggctga accacgagaa
     2281 ccgcattacc ttgatggcgc tcgccgccgg gctgcccggc gtgttgctct cgatgctgtg
     2341 gctcctcggc ggcgactaca cgccgaaggt gaaatggacg ctgggcgcgc tggtgctggg
     2401 ctgttggttc ggcttcgccc tggcgttgcg cgggcgcgtg gtgcgaccgc tgcaaacggt
     2461 ctcgaacctg ctggcggccc tgcgcgaagg cgactactcg atccgcgccc gcggcgcttc
     2521 ataccgcgac gcgctcggcg aagtggtcgt cgaggtcaac gcgctcgggg agacgctgcg
     2581 cgagcagcgg ctcggcgcgc tggaggcggc ggcgctgctg caaacggtga tggccgagat
     2641 cgaggccgcc gtcttcgcct tcgacgccgg cggcaggctg cggctcgtga accgcgccgg
     2701 cgagcggata ctcgcgcagc ccgccgcgcg cctgctcggg cggagggcgg
//
LOCUS       DBNGOH010001022         3215 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4554277, whole genome shotgun sequence.
ACCESSION   DBNGOH010001022 DBNGOH010000000
VERSION     DBNGOH010001022.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3215)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3215)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3215
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4554277"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..672)
                     /locus_tag="AC159P_19870"
     CDS             complement(<1..672)
                     /locus_tag="AC159P_19870"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF012695.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HIR3697755.1"
                     /translation="MRAMILAAGYGTRLWPLTIDRAKPAIPFMGKPLVGYVAEYLARY
                     GCREVAVNLHHRPDSVRRALGDGSRFGVRLRYVEEPVILGTSGALDNAKSILDGDTFV
                     VVNGKIATDIDLSAALETHRRTNALATLVLRPNPARERYSVVEVRDGKVFEFGGMPAS
                     DNAGDVPLMFTGIQILEPRIFEYIPEGVFSHSTTEVYPQAIARGETVAAAQGWPRDRP
                     LRLTKV"
     gene            complement(754..1380)
                     /locus_tag="AC159P_19875"
     CDS             complement(754..1380)
                     /locus_tag="AC159P_19875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697756.1"
                     /translation="MPDENLLATLEQGLRQSRMTRSRLASRLADLEAEAERVRTELNE
                     MDALVSQTEAAMFRILSSMFSSQSAAAPSDAEIEAALRREAVRDALRNSDALRSSHAT
                     GYVNNAPPAVRRDIPHDIPPIRDHVEPKSDRFFERTIPQATTVLLRESGAPLHVNEIY
                     NRLLEGGFKFTGQNPTISIAVSLNRNRRFRKVAPGTFDLTIRDAAQAS"
     gene            complement(1519..2538)
                     /locus_tag="AC159P_19880"
     CDS             complement(1519..2538)
                     /locus_tag="AC159P_19880"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012568.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HIR3697757.1"
                     /translation="MHTSSYTAAVAEAQLTARLVFSLVGRELALVEEEFERQARSNIQ
                     VISYIGDYLRESGGKRVRPALTVLSAYATGGDAARPNVIRMATVMEFLHTATLVHDDI
                     IDNAATRRNRPSINSRFGNQTAVLMGDWLYMSAFETSLGERSLPVLDILTAVTRSMTE
                     GELIQLMLIGRPDISEAQYFDVLQRKTAHLFSACCEIGAILAAAAPREQEALREFGLC
                     LGTAFQLVDDLLDFTSHDDALGKAAGVDLTEGKMTLPVIYLLEEDARWRPSVQAVMRE
                     GNYDEVSRAALLDAAARTGAIDKARARASEYAARAQRALDALPDTKYREALLSIPTYI
                     LERES"
     gene            complement(2694..>3215)
                     /locus_tag="AC159P_19885"
     CDS             complement(2694..>3215)
                     /locus_tag="AC159P_19885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HIR3697758.1"
                     /translation="RGHRAFIEMAQQNSFDIVSQVNRAEIVNALDQTMKEVRQRFDFK
                     GSKAEVVLEKEELILNAEDETKLRNMNDILQQKLVRRGVPLKAFTYGRMEDAAGGTLR
                     QRVAIQQGIPTEKAKEIVRFIKDSKVKVQAAIQGDTVRVSGRDRDTLQDVIAKLKAKD
                     FGIDMQYTNYRSN"
BASE COUNT          521 a          977 c         1102 g          615 t
ORIGIN      
        1 cacttttgtc agcctcaggg gccgatctcg gggccaaccc tgggccgccg ccaccgtctc
       61 cccgcgcgcg atggcttgcg ggtaaacttc ggtcgtcgag tgcgaaaaga ccccttcggg
      121 gatgtactcg aaaatccgcg gctcaagaat ctgaatcccc gtgaacatca aaggcacatc
      181 cccggcgtta tcggatgcgg gcatcccgcc gaattcgaac accttcccgt cgcgcacttc
      241 gaccacgctg tagcgctcgc gcgccggatt gggccggagc acgagcgtcg ccagcgcgtt
      301 cgtcctccgg tgcgtttcga gcgcggcgga gaggtcgatg tcggtcgcga tttttccgtt
      361 gacgacgacg aaggtgtcgc cgtcgaggat gctcttggcg ttgtcgagcg cgccgctcgt
      421 gccgaggatg acgggttctt cgacgtagcg gaggcggacg ccgaagcggc tgccgtcgcc
      481 gagcgcgcgg cgcacggagt cgggccggtg gtgcaggttg acggccacct cgcggcagcc
      541 gtaacgcgcc agatactcgg ccacgtagcc gaccagaggc ttccccatga aaggtatcgc
      601 gggcttggcc cggtcaatcg tcagcggcca caggcgcgtc ccgtaacccg ccgccagaat
      661 catcgcgcgc atagattcac tcagaggcca gaggtcagag gccggaggtc agtaaaagac
      721 agatttccga cctccgacct ccgacctcta cttttatgac gcctgcgcgg cgtcgcggat
      781 ggtgaggtcg aaggtgccgg gggcgacctt gcggaagcgg cggttgcggt tgagcgagac
      841 cgcgatgctg atggtcgggt tctgccccgt gaacttgaag ccgccttcga gcagacggtt
      901 gtaaatctcg ttgacgtgga gcggggcgcc cgactcgcgc aagagcacgg tcgtggcctg
      961 cgggatggtg cgctcgaaga agcggtcgct cttcggctcg acgtggtcgc ggatgggagg
     1021 gatgtcgtgc gggatgtcgc ggcggacggc gggcggcgcg ttattgacgt agcctgtggc
     1081 gtgactgctc ctgagcgcgt cgctgttccg cagcgcgtcg cggacggcct cgcgcctgag
     1141 cgccgcctcg atctcggcgt ccgaaggcgc ggcggctgac tgcgagctga acatggagga
     1201 gaggatgcgg aacatcgccg cctcggtctg gctgacgagc gcgtccatct cgttcagctc
     1261 cgtccgcacg cgctcggcct cggcttcgag gtctgcgagg cgcgaggcca aacgcgagcg
     1321 cgtcatccgc gactggcgca agccctgctc aagcgtcgca agaaggtttt cgtcaggcat
     1381 aagcggctgt gtgctgttag cgattagctg ttagctcttt gagtcggggt tgattgtcgc
     1441 ggaggccatc cctgacggta agacttccgc cgttagtgct ttcaatccaa cacccaagac
     1501 ctaaaaccca ataccctttc agctttctcg ctcaaggatg taggtcggga tcgagagcag
     1561 ggcctctctg tatttcgtgt cgggcagcgc gtcgagcgcg cgttgcgcgc gcgcggcgta
     1621 ctcggaggcg cgggcgcgcg ccttgtcgat ggcgccggtg cgcgcggcgg cgtcgagcag
     1681 cgcggcgcgc gagacttcgt cgtagttgcc ttcgcgcatg acggcttgca cggaggggcg
     1741 ccagcgcgcg tcctcttcga gcaggtagat gacgggcagc gtcatcttgc cttccgtcag
     1801 atcgacgccc gcggccttgc cgagcgcgtc gtcgtgcgag gtgaagtcca gcaggtcatc
     1861 gaccaactgg aacgcggtgc cgaggcagag gccgaactcc ctgagcgcct cctgctcgcg
     1921 gggcgcggcg gcggccagta tcgcgccgat ctcgcagcac gcgctgaaga ggtgcgcggt
     1981 cttgcgctgg agcacgtcga aatactgcgc ctcggagatg tcgggccgcc cgatgagcat
     2041 gagctggatc agctcgccct cggtcatcga gcgcgtgacg gcagtgagga tgtcgaggac
     2101 gggcagcgag cgctcgccga ggctcgtctc gaaggccgac atgtagagcc agtcgcccat
     2161 caggacggcg gtctggttgc cgaagcggct gttgatcgag ggccggttcc gtcgcgtggc
     2221 ggcgttgtcg atgatgtcgt cgtggacgag cgtggcggtg tgcaaaaact ccatcacggt
     2281 cgccatgcgg atgacgttcg ggcgcgcggc gtcgccgccc gtggcgtaag cggagagcac
     2341 cgtcagcgcg ggccgcacgc gcttgccgcc cgactcgcgc aggtagtcgc cgatgtacga
     2401 gatgacctga atgttcgagc gcgcctgccg ctcgaactcc tcctcgacca gcgccagctc
     2461 gcgcccgacg agactgaaga cgaggcgcgc cgtaagttgc gcctccgcga ccgccgccgt
     2521 gtaactcgat gtgtgcatgg ttaatcaaac cgaaagacgt aaaccgtgaa ccgtaaatcg
     2581 tgataagtga aaaaaagtag cgacgagtga cgagtgataa gaggaatgct gttccgactc
     2641 gtcacggttt acggttcacg gttcacggta gttcacggtt tacgtctttc acgttagttc
     2701 gagcggtagt tggtgtactg catgtcgatg ccgaagtctt tggctttgag cttggcgatc
     2761 acgtcctgca aggtgtcgcg gtcgcggccc gagacgcgga cggtgtcgcc ctggatggcg
     2821 gcctgcacct tgaccttcga gtccttgatg aagcggacga tctccttggc cttctcggtc
     2881 gggatgccct gctggatggc gacgcgctgg cgcaaggtgc cgcccgccgc gtcctccatc
     2941 cgaccgtagg tgaaagcctt gagcggcacc ccgcggcgga caagcttctg ctggaggatg
     3001 tcgttcatgt tgcgcagctt ggtctcgtcc tcggcgttga ggatcagctc ctctttttcg
     3061 agcacgacct cggccttgct gcccttgaag tcgaagcgct gcctgacctc tttcatcgtc
     3121 tggtcgagcg cgttgacgat ctcggcccgg ttcacctgcg atacgatgtc gaatgagttc
     3181 tgttgtgcca tctcgataaa tgcgcggtgg ccgcg
//
LOCUS       DBNGOH010001023         2461 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_704167, whole genome shotgun sequence.
ACCESSION   DBNGOH010001023 DBNGOH010000000
VERSION     DBNGOH010001023.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2461)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2461)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2461
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_704167"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(96..1484)
                     /locus_tag="AC159P_19890"
     CDS             complement(96..1484)
                     /locus_tag="AC159P_19890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017834898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase PhoX"
                     /protein_id="HIR3697759.1"
                     /translation="MSKVNRRSFLRRGALAGGALAVQGLAACGARRAFKGAEASEVKG
                     GYGPLLPAKAVNTGETLLALPEGFAYNVLGRTGSPLSDGRPTPRAHDGMAAFEVRGEL
                     RLVRNHEINNGVGTPGAALGNNPYDPLAGGGTTTLVIDSETREVVRGFVSLSGTLVNC
                     AGGPTPWGTWISCEATVLGPARFRDAEGKEFGGFGRPHGYCFEVAASTDGPVTPEPLR
                     QMGRFIHEAVAVDPKSKIIYETEDRLTAGFYRFLPNKPGLLAAGGRLQMLAVKDRAGY
                     DARTGQKVGAALPVRWVDIEHPDPPEVEQDASAVYKQGLGRGGATFARLEGCWHAAGR
                     VYFTSTNGGDKQLGQVWEYEPKGRDEGRLRLLFESPDAEVLRMPDNLCVSPRGEGSLV
                     ICEDHDGVNRLRGLTPRGHVFDLARNMLPGSENREFAGATFSPDGRTLFVNIQTPGLT
                     LAIWGPWKKGSL"
     gene            2026..>2461
                     /locus_tag="AC159P_19895"
     CDS             2026..>2461
                     /locus_tag="AC159P_19895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697760.1"
                     /translation="MDRSADSLSPAAPHPFAGRLYGVIVLLLFACFLFQLWFHTVRTS
                     VTIDEPAHILAGHRHWQCGDFGINPEHPPLLKLLATTPLNFQTLVEPDWACGSKMTSK
                     FDSFSFGNSFLVDNGVDRVVIPARLAAALMSVLLAVLVFFAAR"
BASE COUNT          388 a          857 c          760 g          456 t
ORIGIN      
        1 acacagtcca gcgcgccgtc gtcccggtcg ccctactccg cgcccgcgtc gagcaggacg
       61 ccgatcagtc ccgcgggttg ttcggcttcg gacacctaca acgaaccttt cttccacggc
      121 ccccagatgg cgagcgtgag gcccggcgtc tggatgttga cgaacagcgt ccggccgtcc
      181 gggctgaagg tcgcgcccgc gaactccctg ttctcggagc ccggcaacat gttgcgcgcc
      241 aggtcgaaga cgtgcccgcg cggcgtcagg ccgcgcagcc ggttgacccc gtcgtggtct
      301 tcgcagatga cgagcgagcc ttcgccccgc gggctgacgc agaggttgtc gggcatccgc
      361 agcacttcgg cgtccggcga ctcgaaaagc agacgcagcc gcccttcgtc cctgcccttc
      421 ggctcgtact cccagacctg cccgagctgc ttgtcgccgc cgttggtcga agtgaagtag
      481 acgcggcccg ccgcgtgcca gcagccttcg agccgcgcga aggtcgcgcc gccgcgcccc
      541 aagccctgct tgtagaccgc cgacgcgtcc tgctccacct cgggcgggtc cgggtgctcg
      601 atgtccaccc acctgacggg cagcgccgcg ccgaccttct ggcccgtgcg cgcgtcgtaa
      661 ccggcgcggt ctttgacggc gagcatttgc agccgtccgc ccgctgcgag aagccccggc
      721 ttgtttggaa ggaagcggta aaagcccgcc gtcagcctgt cctcggtctc gtagatgatc
      781 ttcgatttcg gatcgacggc gacggcctcg tggatgaagc gccccatctg ccgcagcggc
      841 tcgggcgtga ccgggccgtc cgttgaggcc gcgacctcga agcagtagcc gtgcgggcgg
      901 ccgaagccgc cgaactcctt cccttcggca tcgcggaagc gcgcggggcc gagcaccgtc
      961 gcctcgcaac tgatccaggt gccccacggc gtcggcccgc ccgcgcagtt gacgagcgtg
     1021 ccgctcagcg agacgaagcc gcggacgacc tcgcgcgtct ccgagtcgat gacgagcgtc
     1081 gtcgtgccgc cgcccgccaa agggtcgtaa gggttgttgc cgagcgcggc gcccggcgtg
     1141 ccgaccccgt tgttgatctc gtggttgcgc accagccgca gctcgccgcg cacctcgaag
     1201 gcggccatgc cgtcgtgcgc gcgcggcgtc gggcgcccgt ccgacagcgg ggagccggtc
     1261 ctgccgagca cgttgtaggc gaacccctcc ggcagcgcga gcagcgtctc gcccgtgttc
     1321 acggccttcg ccgggaggag cggcccgtag ccgcctttca cttccgacgc ctccgcccct
     1381 ttgaaagcac gacgggcgcc gcacgccgcg agcccttgta ctgccagcgc gccgcccgcc
     1441 aacgcgcccc ggcgcaggaa tgatctccgg ttgactttgc tcatgtgtcc actcggcgcg
     1501 gcattcgccg tcacccgtaa tgccaaggta cgatatgcta cacgtgcatt tacggcgccg
     1561 tccatagccg aacagccgac acccttgatt cacccgtgag cttcgggtag caggccgttc
     1621 gagccggacc gtgaacctgc cgacgagact cggacgcgcc atcactatct tcgaccgagt
     1681 cgaggcgggc gattgatagc gatctcaccc ggaactcgag aatgggtacg attccttctc
     1741 ctttgcggac ccatctcgtt ggtagacgat taggtcacgt ccattcagcc taatccatgt
     1801 caggcggcgg gcgtatactt acccacctaa tccggcacca catgcggtta aaaatcattg
     1861 actcgccgcc ccgcatgcct tacaactgcc gcccgaactg ttagcttgaa acctgtttgg
     1921 ttgattgcgc ggcttgtgac gccgggcgtc aggcgggtga cggcgggcgt tatacggacc
     1981 ccgcaacaga gaaagtgtcg attgaaactt gatcgaagaa tcttgatgga cagatcggct
     2041 gattcattgt cacccgccgc gccacacccg ttcgcgggaa gattgtatgg cgtcatcgtc
     2101 ttactgctgt tcgcctgctt cctcttccaa ctctggttcc acaccgtgcg gacgagcgtc
     2161 acgattgacg aacccgcgca tatcttagcc ggccacaggc attggcagtg cggcgacttc
     2221 ggcattaacc ccgagcaccc gccgcttctg aaattgctcg cgacaacccc cctcaatttt
     2281 caaacactcg tcgagccgga ttgggcctgc gggtcgaaga tgacctcgaa attcgacagt
     2341 ttttctttcg gcaattcttt cctcgtcgat aacggcgtgg accgcgtcgt gattccggcc
     2401 cggctcgcgg cggcgttgat gagtgtgctg ctggcggttc ttgtattttt cgcggcgcgg
     2461 g
//
LOCUS       DBNGOH010001024         4871 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4554406, whole genome shotgun sequence.
ACCESSION   DBNGOH010001024 DBNGOH010000000
VERSION     DBNGOH010001024.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4871)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4871)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4871
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4554406"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(207..413)
                     /locus_tag="AC159P_19900"
     CDS             complement(207..413)
                     /locus_tag="AC159P_19900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697761.1"
                     /translation="MLLSITASLAAGGFLLALVSDGFAYELSRVVEKVFDMIEYRLAL
                     QARRRGTVQPRERVAYEATYSHAG"
     gene            complement(680..1756)
                     /locus_tag="AC159P_19905"
     CDS             complement(680..1756)
                     /locus_tag="AC159P_19905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015126267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HIR3697762.1"
                     /translation="MSSSANLEEVEVRAGEGAAPQGAPPEAALMQMILGSLVSQAVYV
                     AAKLSVADLLAEGPRTAAELAEATGAHAPSLYRVMRALASCGVFAERGDGRFELTPAA
                     ELLRSDARNSLRDSAVFMGEEWHWRVWGHTLHSVMTGAPAWEQTHGAEVFPYFAANPG
                     PAAIFDRAMTSMTHAAAEAVTEAYDFSGVERIVDVAGGHGRLLTGILRSNPATRGVLF
                     DLPHVIRGAHSVVENEGVAGRVELVTGDFFESVPEGADAYVMKHIIHDWDDERALAIL
                     KNIARAMRADGKVLLVEMVVAPGNEAHPGKILDLEMLVSPGGKERTEAEYAALFRGAG
                     LRLARVVPTKSPFSVIEAVREWGS"
     gene            complement(2032..3954)
                     /locus_tag="AC159P_19910"
     CDS             complement(2032..3954)
                     /locus_tag="AC159P_19910"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002614549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HIR3697763.1"
                     /translation="MKRLLCLSFTLALIVVCAAPRVAAQSAQQQQAVDNVRARYTKYE
                     YRIPVRDGARLFTAVYVPKDASRPYPFMFIRTPYSVGPYGVDNYRASLGPSEHFEREG
                     FIFVYQDARGRYMSEGEFSQVRPHVPVKRGPRDIDESTDTYDTIEWLLKNVANHNGRV
                     GMWGVSQPGFHVAASIIDSHPALKAASPQAPTADYYMGDDVYHNGAFMLAANFGFYAN
                     FLPHAEPAPPRQRVPFDPGTPDAYEFYLNLPPLAEVNRRLLKGEARYWQEIVDHTTYD
                     DFWKRRSLWRFMDNVKAAVLNVGGWFDAEDPVGPFHIYHAVERKNPGAVNLLVMGPWP
                     HGGWSRGEGSRLGNVSFALKSAEVFREQIQFPFFMHHLKDKTAPLPEAFMFLTGINEW
                     RGHETWPPKNLKPLKLYLNAGGRLTEEAPSAARADAFDEYVSDPNRPVPYLGYIIGGF
                     EDYMTEDQRFASQRPDVLTYVTEPLEEDLIIAGPVKVALNVSTTGTDADFVVKLVDVY
                     PATYPQPAAAQGQQLPPNFVRMGGYQQLVRGEPFRGKYRNSFERPEPFRPGSPAAINF
                     ELPDVYHSFRRGHRLMVQVQSSWFPLVDRNPQKFMEIPKATRADFRKATHRVHRARGL
                     DSSITLSVEGAAARLP"
     gene            complement(4146..>4871)
                     /locus_tag="AC159P_19915"
     CDS             complement(4146..>4871)
                     /locus_tag="AC159P_19915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697764.1"
                     /translation="GKLVSEINNVAKERGLNRVNWNLRYGGPQVRRPPTDEETQFTGG
                     PRGPQVLPGTYTVKLFVGDKAYEKKVEVRLDPTISVQPADLQAQLDMALKLRDMQSAT
                     NSALRTLDSLKGQIEFVERTVKDRLTEVPKDLADKLAEQKKKVEQLQNKLAQPEGGLG
                     FSGRSQLVNRIGGLFFTIDGANAAPTPAQRELYADVQKEFDTKTAEVNKFLGDAVPEL
                     NETLKRFNAPTLMTGKPIELPKP"
BASE COUNT          786 a         1585 c         1622 g          878 t
ORIGIN      
        1 cggcgagccg ctcttcgact cgctccgcag caacccgcgc ttccgccgca tcctcgacag
       61 cgtccacccg ccgggaagag aagggtaaaa ggtaaaaagg ggaaaaggcg aaaaggaaga
      121 agggcgaaga gggggagaca cgtccctctt cgccctttcc ttcttccctt ttccccttat
      181 taccttttac ccttagcccg cagcgcttat ccggcgtgcg aataagtggc ttcgtacgcg
      241 acccgctcac gcggttgaac ggtgccgcgc ctgcgcgcct gaagcgccag cctgtactcg
      301 atcatgtcga acaccttctc gacgacgcgc gacagctcgt aggcgaatcc gtcggacacg
      361 agcgccagca gaaagccgcc cgccgcgagc gacgccgtga ttgatagcag catgattaac
      421 atttcccatc tcgcctccac ttttacgtac tcaacccaat cccttcccaa ctattaacgc
      481 gcgaaaaggc ggcccgaggg atcacgtctc gccttttttt gttgggccgc gcgaacaggc
      541 ggcccacgcg aagaacggcg ggcggcgcat ccgcttgcgc cgcccgccgt gttattaacc
      601 cccgtgtatt cggcccttgc ccgttcgtcc cgccctttcg ttcggccccc gcccccgctc
      661 gccaaagtcc ggggcgctct tagctgcccc actccctgac cgcctcgatg acgctgaacg
      721 gcgatttggt cggcacgacg cgcgcgaggc gcaggcccgc gccgcggaag agcgccgcgt
      781 actcggcctc cgtgcgctcc ttgccgccgg gagagaccag catctcaagg tcgagaatct
      841 tgccggggtg ggcttcgtta cccggcgcga cgaccatctc gacgaggagc accttgccgt
      901 cggcgcgcat ggcgcgggcg atgttcttga ggatggcgag cgcgcgctcg tcgtcccagt
      961 cgtggatgat gtgcttcatc acgtaggcgt cggcgccttc gggcaccgac tcgaagaagt
     1021 cgcccgtgac gagttcgacg cgcccggcca ccccctcgtt ttcgaccacg gagtgcgcgc
     1081 ccctgatgac gtgcggcagg tcgaagagca cgccgcgcgt cgcgggattc gaacgcagga
     1141 tgcccgtcag cagacgcccg tggccgcccg ccacgtcaac tatcctctcg acgccggaga
     1201 agtcgtaagc ctccgtgacc gcctcggcgg cggcgtgcgt catgctcgtc atggcgcggt
     1261 cgaagatggc cgcggggccg gggttcgcgg cgaagtaggg gaagacctcc gcgccgtgcg
     1321 tctgctccca cgccggggcg cccgtcatca cgctgtggag cgtgtggccc cacacgcgcc
     1381 agtgccactc ctcgcccatg aagacggcgc tgtcgcgcag cgagttgcgc gcgtccgagc
     1441 gcagcagctc cgccgcaggg gtcagctcga accgcccgtc cccgcgctcg gcgaagaccc
     1501 cgcacgaggc gagcgcccgc atcacgcggt acagcgacgg cgcgtgggcg cccgtcgcct
     1561 cggccagctc ggcggcggtg cgcggcccct ccgcgagcag gtctgcgacc gacagcttgg
     1621 ccgccacgta gaccgcctgc gagacgagac tgcccagtat catctgcatc aacgcggcct
     1681 ccggcggcgc accctgcggc gcggcccctt cgcccgcgcg cacctcgact tcttctaaat
     1741 ttgctgagct agacattgat ttttcccctt caaacgatga tatgccgggc cggccaaacg
     1801 accccttacg aactaacgcg caaacgaagc gtgagaggga tcacgaagaa agtaggcagt
     1861 aggcagtagg cggtaggcag taggcagtca agtgagaagg gggaagaggc tgggggggta
     1921 aagtctcggg cctctccgct cttcaagcaa gaaagcaaga ggcttgaaaa atataaactc
     1981 aagcctcttg tttttactgc ctactgccta ccgcctactg cctactttct tctacggcag
     2041 ccgcgccgcg gcgccttcga cggagagcgt gatggaagag tcgaggcccc gtgcgcggtg
     2101 gacgcggtgg gtggccttcc ggaagtcggc gcgcgtggcc ttcgggattt ccatgaattt
     2161 ctgcgggttg cggtcaacaa gcgggaacca cgaactctgc acctgcacca tcaggcggtg
     2221 gccgcggcgg aaggagtggt acacgtccgg aagctcgaag ttgatggccg ccgggctgcc
     2281 gggcctgaac ggctccggcc tctcgaagct gttgcgatac ttgccgcgga acggctcgcc
     2341 gcgcacgagc tgttgatagc cgcccatccg cacgaagttc ggcggaagct gttgcccctg
     2401 cgccgccgcg ggctgcgggt aggtcgccgg gtacacgtca acgagcttga cgacgaagtc
     2461 cgcgtccgtc cccgtcgtcg agacgttgag cgcgactttg acggggccgg cgatgatcaa
     2521 gtcctcttcg agcggctccg tgacgtaggt cagcacgtcc gggcgctgcg aggcgaagcg
     2581 ctggtcttcg gtcatgtagt cctcgaaccc gccgatgatg tagccgaggt agggcacggg
     2641 gcggttcggg tcgctcacgt attcgtcgaa tgcgtcggcg cgcgccgccg acggcgcttc
     2701 ctcagtcagc cgcccgcccg cgttcaggta gagcttcagc ggcttcaggt tcttcggcgg
     2761 ccacgtctcg tggccgcgcc actcgttgat gcccgtcagg aacatgaagg cttcggggag
     2821 cggcgcggtc ttgtctttga gatggtgcat gaagaagggg aactgtatct gctcgcggaa
     2881 gacctcggcg gacttgaggg cgaacgagac gttgccgagg cggctgccct cgccgcgcga
     2941 ccacccgccg tgcggccacg gccccatcac gagcagattg accgcgccgg ggttcttccg
     3001 ctcgaccgcg tggtagatgt ggaacggccc gaccgggtct tcggcgtcga accagccgcc
     3061 gacgttcagc accgccgcct tcacgttgtc catgaagcgc cacagcgagc gccgcttcca
     3121 gaagtcgtcg taggtcgtgt ggtcaacgat ctcctgccag tagcgcgcct cgcctttgag
     3181 aagcctccgg ttgacttcgg cgagcggcgg caggttgaga taaaactcgt aggcgtcggg
     3241 cgtgccgggg tcgaagggga cgcgctggcg gggcggcgca ggctccgcgt gcggcaggaa
     3301 gttggcgtag aagccgaagt tggcggcgag catgaaggcg ccgttgtggt acacgtcgtc
     3361 gcccatgtag tagtcggcgg tgggcgcctg cggcgaggcg gctttcaagg ccgggtgcga
     3421 gtcgatgatg ctcgccgcga cgtggaagcc cggctgcgag acgccccaca tgccgacgcg
     3481 cccgttgtgg ttcgccacgt tcttcagcag ccactcgatg gtgtcgtacg tgtcggtgct
     3541 ctcgtcgatg tctcttggcc cgcgcttcac cggcacgtgc ggtcgcacct gcgagaactc
     3601 gccctcggac atgtagcgcc cgcgcgcgtc ctggtagacg aagatgaagc cctcacgctc
     3661 gaagtgctcc gaggggccta agctcgcgcg gtagttgtcc acgccgtacg ggccgacgct
     3721 gtagggcgtg cggatgaaca tgaacgggta gggccgcgac gcgtccttcg gcacgtagac
     3781 ggcggtgaac agccgcgcgc cgtcgcgaac ggggatgcgg tattcgtact tcgtatagcg
     3841 cgcgcggacg ttatcgaccg cctgctgctg ctgcgcgctc tgcgccgcga cgcggggcgc
     3901 ggcgcagact acgatgagcg cgagcgtgaa cgataagcaa agaagccttt tcatgattgc
     3961 acaagagaac tgcggtgcgc ggttcgaaga ttcaacgtgc caacggctgt tggggcaggg
     4021 cttgtccgcg gcccgttgcg atgcgatgga ttcagtcttg agtttaccgc ggcggcgggc
     4081 agggacaagc cctgccccac gattcatttc ggcgacccgc gttcatcccg acggcccgtg
     4141 ccgcgtcacg gcttcggcag ctcgatgggc ttgcccgtca tcaaggtcgg cgcgttgaag
     4201 cgtttgagcg tctcgttcaa ttccggcacg gcgtcgccga ggaacttgtt gacctcggcg
     4261 gtcttggtgt cgaactcctt ctgcacgtcg gcgtagagtt cgcgctgcgc gggcgtgggc
     4321 gcggcgttcg cgccgtcgat ggtgaagaag aggccgccga ttctattgac gagctgcgac
     4381 cggcccgaga agccgaggcc gccttcgggc tgcgcgagct tgttctgcaa ttgctcgacc
     4441 ttcttcttct gctcggcgag cttgtccgcc aaatccttcg gcacctccgt cagccgatcc
     4501 ttcacggtgc gctcgacgaa ctcgatctgg cctttgaggc tgtcgagcgt gcgcagcgcg
     4561 ctgttcgtcg ccgactgcat gtcgcgcagt ttgagggcca tgtcgagctg cgcctgaagg
     4621 tcggcaggtt gcacggagat ggtcgggtcc agacgcactt cgactttctt ctcgtaagcc
     4681 ttgtcgccga cgaagagctt gacggtgtag gtgccgggca gcacttgcgg gccgcgcggg
     4741 ccgccggtga actgcgtctc ctcgtccgtg ggcgggcggc gcacctgcgg gccgccgtag
     4801 cgcaaattcc agttgacgcg gttcaagccc cgctccttgg cgacgttgtt gatttcggaa
     4861 acgagcttgc c
//
LOCUS       DBNGOH010001025         3845 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4738365, whole genome shotgun sequence.
ACCESSION   DBNGOH010001025 DBNGOH010000000
VERSION     DBNGOH010001025.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3845)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3845)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3845
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4738365"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..357
                     /locus_tag="AC159P_19920"
     CDS             <1..357
                     /locus_tag="AC159P_19920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017286876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HIR3697765.1"
                     /translation="DFIRMSREPGTVILDARSRQKYDELHVKGALNLSFPDIAVESLG
                     RVLPDKDTRVLIYCNNNFRGAEGPFPTKRSIASLNLSTYIALYSYGYRNVYELGPLID
                     VQATKLELVPSPPDRL"
     gene            798..1274
                     /locus_tag="AC159P_19925"
     CDS             798..1274
                     /locus_tag="AC159P_19925"
                     /inference="COORDINATES: protein motif:HMM:NF025887.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YybH family protein"
                     /protein_id="HIR3697766.1"
                     /translation="MKRAALIAFLLAGSFVASGQEAPAAPPAAEEARAAIAANHSKFI
                     GAFGKGDAAAVAALHAADARLLPHGGRPVEGVQNVEAFWAGLLKAGARLVQLQTLSVE
                     ERGELAYEVGDYIVTTRPQPGLTAINSGSYLVVWRRQGGEWKLAAAIWNTDKAPER"
     gene            complement(1317..1874)
                     /locus_tag="AC159P_19930"
     CDS             complement(1317..1874)
                     /locus_tag="AC159P_19930"
                     /inference="COORDINATES: protein motif:HMM:NF012451.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HIR3697767.1"
                     /translation="MTEQNTLDYYEVLQVSANAEPETINRVYRMLAQRFHPDNQATGN
                     EPRFRALLEAYTVLSDPEKRARYDVAHQRQRQDRWRLVSAGAQAENDFEMEQAARLTV
                     LEVLYAKRRLEPNDPGIFTLDLEELTGRPREHLEFTVWFLLQKKFVTIGDNSRLLLTA
                     EGAEYLELNYRNLIQQKRLRASNPE"
     gene            2070..2900
                     /locus_tag="AC159P_19935"
     CDS             2070..2900
                     /locus_tag="AC159P_19935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LolA-like protein"
                     /protein_id="HIR3697768.1"
                     /translation="MTSARVPISCGGAILRVALAVAATALAGCGGGAPRGGAGTPKPT
                     PTPSPDQAQVVEAIFSRYAEAVGGQEAIDRVESYVLKGRFEMTGSDLKLPVEVYFEKP
                     DKSLMVINVPRVGVIRRGRAGGGRAWVQTPFAAGARATGDSPDELAEVERDHDLYKAG
                     RIRELYQEVRLEGKARLKGRDVYVVEGKPAEGPAEKMLFDVETGLLLRWDVVRRAPGR
                     PNVFARVYLEDYREVGRVKVPFTVRYYFEPREMILRLEEVKHDVPLDDAMFERPQGSR
                     "
     gene            2981..3187
                     /locus_tag="AC159P_19940"
     CDS             2981..3187
                     /locus_tag="AC159P_19940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697769.1"
                     /translation="MKFIEAAHPEGGSVAIHIDHIASAHYKPSASKDIKTRLTIDLDN
                     SEQDVVLLGEEADRVWAIIKGLML"
     gene            3421..3633
                     /locus_tag="AC159P_19945"
     CDS             3421..3633
                     /locus_tag="AC159P_19945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697770.1"
                     /translation="MPISLRAIRHYVAALALCLAAVVVFTREIGPAEWVALLNPFLFL
                     LLDIREERCRLKALEDGLNALDETGA"
BASE COUNT          653 a         1257 c         1303 g          632 t
ORIGIN      
        1 gacttcatcc gcatgagccg ggagccgggc accgtcatcc tggacgcgcg gagcaggcag
       61 aagtacgacg agctacacgt caaaggtgcg ctcaatctga gcttccccga catcgcggtc
      121 gaaagtctcg gtcgggtgct ccccgacaaa gacacccgcg tcctcatcta ctgcaacaac
      181 aatttccggg gcgcggaggg gccgttcccg acgaagaggt cgattgcctc gctcaacctc
      241 tcgacgtaca tcgccctgta cagctacggc taccggaacg tgtacgagct ggggccgctg
      301 atcgacgtgc aggcgaccaa actggaactc gtgccttccc cgcccgacag gctgtagcga
      361 aggcagccga cataccactc cgcgcgcact cggccacggg gccgggagca ggcgttcgtt
      421 cggcttcggc ggtgtgaaaa gagatgaggg ggcggcccat catgccgccc cctcatctca
      481 agggagagat gctcgccctt gaccccaatg ccgacactct acacgaacgg gcggcggccc
      541 gcatcggaca aatgactaat tcctcgtagt agaaatgtca cggggccgcg gcttgaagct
      601 gcggctcccc tttgtcgatt gccgacgggg tgccgcggcg gcgcggcccg cgaagccgtc
      661 gggctggctt cgcggacggt ctcgcggaga gtgtcggaga tatgtgctag agtcttgctt
      721 caaggtgtct tcctgatggg gccgcctgac aacggcgcgg cgcccgtcgc gcgactctcc
      781 cgatgcggag gacaaacatg aagcgagccg cactgatcgc cttcctgctg gcggggtcgt
      841 tcgtcgcgtc cgggcaggag gcgcccgccg ccccgcccgc cgccgaagag gcacgcgccg
      901 ccatcgcggc caaccacagt aaattcatcg gcgccttcgg caagggcgac gccgcggcgg
      961 tggccgccct gcacgccgcc gacgccaggt tgctgccgca tggcgggcgt cccgtcgagg
     1021 gcgtccagaa cgtagaggcg ttctgggcgg ggctgttgaa ggcgggggcg aggctggtgc
     1081 agttacagac cttgagcgtg gaggagcggg gcgagttggc ctacgaggtc ggcgactaca
     1141 tcgtgaccac gcggccccag ccgggcctga ccgcgatcaa ctcgggcagc tacctcgtcg
     1201 tatggaggcg gcagggcggg gagtggaaac tggccgcggc gatctggaac acagacaagg
     1261 cgcccgagcg gtgaagcgtc tctggccgga gcgaatgaag gcgtggtcgc cgatgcttac
     1321 tcggggttcg aggcccgcag gcgtttctgc tgaatgaggt tgcggtagtt cagctcaagg
     1381 tattcggccc cttcggcggt cagcagcagg cgcgagttgt cgccgatggt gacgaacttc
     1441 ttctggagga ggaaccagac ggtgaactcc agatgctcgc gcggcctgcc ggtcagctct
     1501 tcgaggtcga gcgtgaagat gccggggtcg ttcggctcca ggcgccgctt ggcgtagagg
     1561 acttcgagca cggtcagccg ggcggcctgc tccatctcga agtcgttctc ggcctgcgcg
     1621 ccggcggaca cgagccgcca gcgatcctgc cgctgccgct ggtgcgcaac gtcgtagcgc
     1681 gcccgctttt ccgggtcgct caacaccgtg taggcttcga gcagtgcgcg gaagcgcggt
     1741 tcgttgccgg tcgcctgatt gtccgggtgg aaacgctgcg cgagcatccg gtagacgcgg
     1801 ttgatcgtct ccggctcggc gttcgcgctg acttgcagca cttcgtaata atcgagcgtg
     1861 ttctgttccg tcataccatg cgggactcgc cggcgaatgc taacccgccc cacggtcggc
     1921 gtcaaaccgc accgggcgac aggtcttacg cggttcgctc ggggccgtgt atcatcgggc
     1981 gccgacaatc agcccgcagg tcgcccgttg acgggcgtgc cgcgaagaaa tattgtttgc
     2041 ttctctcgac acctgatgcc ggtcgcgaca tgacttcagc gcgcgtacca atctcctgcg
     2101 gcggcgcgat tctccgggtg gcgctggcgg tcgccgcgac ggccctggcc ggctgcggcg
     2161 gcggcgcccc ccgcggcggc gcaggcacgc ccaagccgac gcccacgccc tccccggatc
     2221 aggcgcaagt cgtcgaagct attttcagcc gatacgccga ggccgtcggc gggcaggagg
     2281 ccatcgaccg ggtcgagagc tatgtcctca aaggccggtt cgagatgacg ggcagcgact
     2341 tgaagctccc cgtcgaggtc tacttcgaga agccggacaa gagcctgatg gtgatcaatg
     2401 tcccgcgcgt cggggtcatc cgcaggggcc gggccggcgg cggtcgggcg tgggttcaga
     2461 cgccgttcgc ggcgggcgcg cgcgcgactg gcgacagccc cgacgaactc gcggaggtcg
     2521 agagggatca cgatctctac aaggcgggcc ggatcaggga gctgtatcag gaggtgaggc
     2581 tcgaaggtaa ggcgcggctc aaggggcgcg acgtgtacgt ggtcgagggc aagcccgccg
     2641 aaggccccgc cgagaagatg ctgttcgacg tggagacggg gctgctgctg aggtgggacg
     2701 tggtgcggcg tgcgccgggc cggcccaacg tcttcgcccg cgtctacctc gaagactacc
     2761 gcgaggtggg ccgggtcaag gtgcccttca ccgtcaggta ctacttcgag ccgcgcgaga
     2821 tgatcctccg cctcgaagag gtgaagcacg acgtgccgct cgacgacgcg atgttcgagc
     2881 ggccacaagg cagccgttga cggcgcggcg cggagaaggg gcggcccctt cccccggcgc
     2941 gccttcgcat cattccgacc gcagaggaga catttcacac atgaagttca tcgaggcggc
     3001 ccacccggag ggcggcagcg tcgccatcca catcgaccat atcgccagcg cacactacaa
     3061 gccgtccgcg agtaaagaca tcaaaactcg cctgaccatc gacctcgaca acagcgagca
     3121 ggacgtggtg ctcctcggcg aggaggccga ccgcgtgtgg gcgatcatca aggggctgat
     3181 gctgtagagt cgccgcgctc gccgcagggc ggggccgggt gtgggcggcg cgaaaaggga
     3241 gtgaccggcg cggggcgcgg gggcgctcct tccggcgtgg aaacagccgc gcattgtccc
     3301 ttttatcgag aggtaactcg gacgccgaaa ggatcgcccc atgatcaacg ttctactgtg
     3361 cctcgcggtg ctcagcttct ccatcctcgt ctcgctggcc gtcggcgccg tggagaagag
     3421 atgccgataa gtcttcgcgc catccgccac tacgtcgccg cgctcgcgct ctgtctcgcg
     3481 gcggtcgtcg tcttcacgag ggagatcggc cccgccgagt gggtcgccct gctcaacccg
     3541 ttcctcttcc tgctgctcga catacgggag gagcgctgcc gcctgaaggc gcttgaggac
     3601 ggcctgaacg ccctcgacga gaccggcgca tgaccgccct cttccgacgc ccgcctctaa
     3661 cacaccccat gacgcggcgc ggcctctcta aagccgtgga gtcacgcggt caaacgagaa
     3721 ttccccccca atcgctcaca accggacaaa taatgactga ataacggtca cattttttgt
     3781 caccttccga gtcccgaccg tcctcctccc gacccgccaa cccgagtttc cagcgacttt
     3841 cctct
//
LOCUS       DBNGOH010001026         2756 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4738430, whole genome shotgun sequence.
ACCESSION   DBNGOH010001026 DBNGOH010000000
VERSION     DBNGOH010001026.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2756)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2756)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2756
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4738430"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            95..1483
                     /gene="rseP"
                     /locus_tag="AC159P_19950"
     CDS             95..1483
                     /gene="rseP"
                     /locus_tag="AC159P_19950"
                     /inference="COORDINATES: protein motif:HMM:TIGR00054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HIR3697771.1"
                     /translation="MSYIISGIAFILILGAAVVIHEFGHFIVAKLLGIRVETFSVGFG
                     KRLWGRRWGTTDYRLSLIPLGGYVKLGGDESNAGLEEGAGEEIPEHERFDRRPRWQKF
                     LVGVAGPVMNILTAIAVPLGIAMTVGAPVMPPPVVKSVPAGSPAEAAGLKPGDRIVAF
                     NGRENSNWEKIRNDAMISPDQQIPLVVERGGQRVPLTITPKKEMMDGESFGVLGIDPD
                     YGDVPIIISSIVEGSPAAESGLQPGDQIVRLGGGEVHSAEQVVEYIRQHKTGPVAFTV
                     RRNGATQDIVAGAERRLPDGKLGLVPDEAPPPKRLGLADAASYAVARNVEMLRVTGLA
                     LGQIFSGQRSVRDTVSGPIGIAVASKRAVEEGGLEGGLHLLGFLSLNLGIFNLLPIPV
                     LDGGMIFMLFVEGFLALFNKKLSMTVRERIQQVGFVFLLLLMGFVIINDVTKQFTRFR
                     SSGEPPAATQQK"
     gene            1581..2687
                     /gene="ispG"
                     /locus_tag="AC159P_19955"
                     /gene_synonym="gcpE"
     CDS             1581..2687
                     /gene="ispG"
                     /locus_tag="AC159P_19955"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HIR3697772.1"
                     /translation="MKQTRAVRVGKVQVGGGAPVSVQSMTKTDTADVGGTVRQIEEMV
                     RAGCEIVRVAVPDKEAAVALKEIRRRTPAEVPLVADIHFHYKLALLALEAGVDKLRLN
                     PGNIGSIERVREVVRAAAAQAVPIRIGVNGGSLEKDLLKKYGTATPEAMVESAERHIR
                     ILEDLDFREIIVSLKASDVHRTVAAYRLLSEKVDYPLHLGVTEAGTAFGGTIKSAIGL
                     GVLLHEGIGDTIRVSLAAEPQEEVRVAWEILKSLDLRKRGVTVVACPTCGRLDVDDFV
                     GIVTEIERRLAHIEEPLHLSIMGCAVNGPGEAHDSQLGVTFGRGVGMIFKDGKPLRKV
                     AGADIVEAFVAETEKLVAEGGAAQEPVPELVTIK"
BASE COUNT          470 a          933 c          918 g          435 t
ORIGIN      
        1 ctcggcactt acactggctg ctgtgcaacc atgaaggggg cttgagactc taaaccgctg
       61 agtttttcgg ggcatcacgg gttccaatca tcttatgagc tacattatct ccggcatcgc
      121 tttcatcctc atcctcggcg cggcggtcgt catccacgag ttcggccact tcatcgtcgc
      181 caagcttctg gggattcgcg tcgagacctt ctcggtcggc ttcggcaaac gcctgtgggg
      241 ccgccggtgg ggcacgaccg actaccgctt gagcctgatt cccctcggcg gctacgtcaa
      301 gctcggcggc gacgagtcga acgcgggcct cgaagagggc gcgggcgaag agattcccga
      361 acacgagcgc ttcgaccgcc ggccccgctg gcagaagttc ctcgtcggcg tggccggccc
      421 ggtcatgaac atcctgacgg ccatcgccgt gccgctcggg atcgcgatga ccgtcggcgc
      481 gccggtgatg cccccgcccg tcgtcaagag cgtcccggcg ggcagccccg ccgaggccgc
      541 gggcctcaag ccgggcgacc gcatcgtcgc cttcaacggg cgcgagaaca gcaactggga
      601 gaagatcagg aacgacgcga tgatctcgcc cgaccagcag atcccgctcg tcgtcgagcg
      661 cggcggccag cgcgtcccgc tcactatcac gcccaagaag gagatgatgg acggcgagtc
      721 gttcggcgtc ctcggcatcg accccgacta cggcgacgtg ccgatcatca tcagttccat
      781 cgtcgagggt tcgcccgccg ccgagagcgg gcttcagccc ggcgaccaga tcgtccgcct
      841 cggcggcggc gaggtgcaca gcgccgagca ggtcgtcgag tacatccgcc agcacaagac
      901 cgggccggtc gccttcaccg tccgccgcaa cggcgcgacg caggacatcg tcgcgggcgc
      961 cgagcggcgg ctcccggacg gcaagctcgg cctcgtgccg gacgaggcgc cgccgccgaa
     1021 gcggctcggg ctggcggacg cggcctccta cgccgtcgcg cgcaacgtcg agatgctgcg
     1081 cgtgacgggc ctcgcgctcg gccagatttt cagcggccag cgctcggtgc gcgacacggt
     1141 gtcggggcca atcggcatcg cggtggcttc gaagcgcgcg gtcgaagagg gcggcctcga
     1201 aggcgggctg cacctcctgg gcttcctcag cttgaacctc ggcatcttca acctgctgcc
     1261 gatcccggtg ctcgacggcg gcatgatctt catgctcttc gtcgaaggct tcctcgcgct
     1321 cttcaacaag aagctctcga tgactgtgcg cgagcgcatc cagcaggtcg gcttcgtctt
     1381 cctcctgctc ctcatgggct tcgtcatcat caacgacgtg accaaacagt tcacgcgctt
     1441 ccgctcgtcg ggcgagccgc ccgcggcgac gcagcagaaa tgagaactac cgtgagccgt
     1501 aaaccgtaaa ccgtgatgag aggacagagg gttttcactt atcacggttg acggtttacg
     1561 gttcacggca tttgaacggt atgaaacaaa cgagggcagt gcgggtcggg aaggtgcagg
     1621 tcggcggggg cgcgccggtg tccgtgcagt cgatgacgaa gaccgacacg gcggacgtcg
     1681 gaggcaccgt caggcagatc gaggagatgg tgcgcgccgg gtgcgagatc gtgcgcgtgg
     1741 ccgtgccgga caaggaggcg gcggtcgcac ttaaagagat caggcggcgc acgcccgcgg
     1801 aggtgccgct cgtcgcggac atccacttcc actacaagct ggcgctgttg gcgctcgaag
     1861 cgggcgtcga taagctgcgg ctgaatcccg gcaacatcgg ctccatcgag cgcgtgcgcg
     1921 aggtggtgcg cgccgccgcg gcgcaagccg tcccgatccg catcggcgtc aacggcggat
     1981 cgctcgaaaa ggacttgctc aagaagtacg gcacggccac gcccgaggcg atggtcgaat
     2041 ccgccgagcg ccacatccgc atcctcgaag acctcgactt ccgcgaaatc atcgtctcgc
     2101 tcaaggcgtc ggacgtgcat cgcacggtcg ccgcctaccg cctgctctcc gagaaggtcg
     2161 attacccgct ccacctcggc gtgaccgagg cgggcacggc cttcggcggc acgatcaaat
     2221 cggccatcgg cctcggcgtg ctgctgcacg aaggcatcgg cgacaccatc cgcgtctcgc
     2281 tcgcggcgga gccgcaggag gaggtgcgcg tcgcttggga gatactgaag tcgctcgacc
     2341 ttcggaagcg cggcgtgacg gtcgtcgcct gcccgacgtg cgggcgcctc gacgttgacg
     2401 acttcgtcgg catcgtcacg gagatcgagc gccgcctcgc ccacatcgag gagcctttgc
     2461 acctctcgat catgggctgc gccgtcaacg ggccgggcga ggcccacgac agccagctcg
     2521 gcgtcacctt cgggcgcggc gtcggcatga tcttcaagga cggcaagccc ctgcgcaaag
     2581 tcgcgggcgc cgacatcgtc gaagccttcg tcgcggagac cgaaaagctc gtcgccgaag
     2641 gcggcgcggc gcaggagccc gtccccgaac tcgtcacgat caagtagacg caagccacat
     2701 cacgaaacgc ccggagccgg ccttgatgaa ggccggctcc gcgcgtttcg ggccgt
//
LOCUS       DBNGOH010001027         4565 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4555120, whole genome shotgun sequence.
ACCESSION   DBNGOH010001027 DBNGOH010000000
VERSION     DBNGOH010001027.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4565)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4565)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4565
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4555120"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..877
                     /locus_tag="AC159P_19960"
     CDS             <1..877
                     /locus_tag="AC159P_19960"
                     /inference="COORDINATES: protein motif:HMM:NF025885.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-54 interaction domain-containing protein"
                     /protein_id="HIR3697773.1"
                     /translation="IVGESGTGKELIANAIHYNSLRAKKPFIKVNCAALPKELIESEL
                     FGHTKGAFTGAHADKDGLIQHAAGGSLLLDEIAEMPVELQPKLLRVLQERSYRKIGSE
                     RTYAVDFRLITSTNRPPADAIREGILRDDLFYRISTITIHVPPLRERTEDIQLLTEFF
                     LKQYSRKYQRQATGISQAAYQRLFAHTWPGNVRELQNVIERAVLLAKGNKIEPVDLPF
                     DNGSIPEKTAQGTGWDVPPNMTLEDIEKLVIERTLQRTGGNKQAAANLLGIYRPRLYS
                     KNKKYNIDIPALIAS"
     gene            complement(986..1360)
                     /gene="queD"
                     /locus_tag="AC159P_19965"
     CDS             complement(986..1360)
                     /gene="queD"
                     /locus_tag="AC159P_19965"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020715258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - tRNA queuosine(34) biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HIR3697774.1"
                     /translation="MFEVMIERNFSSAHQLRGYRGKCENLHGHNYRVEIYARGRELNN
                     IGLLVDFVELKEAADEVVNFLDHRNINELEPFVEELNPSAENLARFILERVARRVGDD
                     RVQIYKVRCYETPTSVATYTVD"
     gene            complement(1526..3511)
                     /gene="yoaA"
                     /locus_tag="AC159P_19970"
     CDS             complement(1526..3511)
                     /gene="yoaA"
                     /locus_tag="AC159P_19970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013570014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair helicase YoaA"
                     /protein_id="HIR3697775.1"
                     /translation="MEEIFGPSGLIARAHPDYEYRPGQVEMAGAVLRAFEERKHLVVE
                     AGTGTGKTLAYLVPAIAAALERRCRVVVSTGTKNLQEQLMEKDIPFLQRVLPRKFTAA
                     YLKGRGNYACLHRIKRAETTPILDGLDDIDYFNEVRQWARESETGDRAELTELPENLS
                     FWRHVDARSDTCLGQKCADFDACFITRMRQRAADADLVIVNHHLFFADLALRGGEYGQ
                     VIPDYGAVIFDEAHQVEDVAAEYFGAQVSNYQVEDVLRDLQMLPLTDVGANRELNRVS
                     TRVARFAEQFWMGFKEGRGEEGRTPIVPGTFARKNRQGEIEATPLGEAYLSLDGALNR
                     METTLDVLRDKPPEVDNLLRRVRELRFNLEFIVAGDDKRYVYWTERRGRGTFLRASPI
                     DVSTLLQDKLFERVETVVLTSATLASAGSFNFVRGRLGLLEETEELIAESGYDYETNA
                     LLYLPPRMPEPRSPQWPQAAADEVVKLLNASEGRAFVLSTSLSGMRDLHERVSREVDF
                     PCFVQGSMSKAGLLDKFRATPNAVLFATSSFWQGVDVRGQQLSCVIVDKLPFAVPTDP
                     VVSARQRFIEEQGGSSFYEYSVPQAIISLKQGLGRLIRSATDRGVLAVLDPRLRTSGY
                     GRLFLQSLPPSRVTTDIGEVEEMFKDQPSMLKVRQPV"
     gene            complement(3818..3994)
                     /locus_tag="AC159P_19975"
     CDS             complement(3818..3994)
                     /locus_tag="AC159P_19975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697776.1"
                     /translation="MPQENEFEANLVDPSEDDGGSKLVDPSEDEGGASAAEQGQEGEI
                     AEPNAMKDGDGPNW"
     gene            complement(4171..>4565)
                     /locus_tag="AC159P_19980"
     CDS             complement(4171..>4565)
                     /locus_tag="AC159P_19980"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807202.1"
                     /note="catalyzes the reaction of glycine with
                     5,10-methylenetetrahydrofolate to form L-serine and
                     tetrahydrofolate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HIR3697777.1"
                     /translation="QGFRLVSGGTDNHLVLVDVFMEGRGVTGKVAEKALEAAGITVNK
                     NTIPFDTNKPFVASGVRLGTPALTTRGMGEAEMREVARLIAEALGAPDSEEVRDRVRR
                     SVRELAASFPLYPKRVRRAPETEAMGAD"
BASE COUNT          775 a         1497 c         1491 g          802 t
ORIGIN      
        1 catcgtcggc gaatcgggca ccggcaagga gctgatcgcc aacgcgattc actacaactc
       61 tctgcgcgcc aagaagccct tcatcaaggt caactgcgcc gcgctcccga aggagttgat
      121 cgaatccgag ctgttcggcc acacgaaagg cgccttcacg ggcgcgcacg cagacaagga
      181 cggcctcatc cagcacgccg cgggcggctc tctgcttctg gatgagatcg ccgagatgcc
      241 tgtcgagcta cagcccaagc tgctgcgcgt cctgcaagag cgcagctacc gcaagatcgg
      301 gtcggaaaga acctacgccg tggacttccg cttgataact tcgacgaacc gcccgccggc
      361 tgacgcgatc cgcgagggca tcctgcgcga cgacctcttc taccgcatct cgaccatcac
      421 gattcacgtc ccgcccctgc gcgaacgcac cgaggacatc cagctcctca ccgagttctt
      481 cctcaagcag tactcgcgca agtaccagcg gcaggcgacg ggcatctcgc aggcggccta
      541 ccagcgcctc ttcgcgcaca cctggcccgg caacgtccgc gaattgcaga acgtcatcga
      601 gcgcgccgtg ctgctggcga agggcaacaa gatcgagccg gtcgatctgc cgttcgacaa
      661 cggctcgatt cccgaaaaaa ccgcgcaagg cacgggctgg gacgtgccgc ccaacatgac
      721 gctcgaagac atcgagaagc tcgtcatcga gcgcacgctg caacgcaccg gcggcaacaa
      781 gcaggcggcg gccaacctgc tcggcatcta ccgcccccgc ctctacagca aaaacaagaa
      841 atacaacatc gacatcccgg cgctcatcgc ctcttgaaag tctggagtcc ggagtccgga
      901 aagaagaagc cggaaaagaa aaacagaagc ccggttgtga acggcaaccg ggcttctgtt
      961 tttcttccgg actccggact ccggactaat ccaccgtgta cgtcgccacg ctggtcggcg
     1021 tctcgtagca gcggactttg tagatttgca cgcggtcgtc gccgacgcgg cgggcgacgc
     1081 gctccaggat gaagcgcgcg aggttctcgg cggaggggtt cagctcctcg acgaacggct
     1141 caagctcgtt gatgttgcgg tggtcgagga agttcacgac ctcgtccgcc gcctctttca
     1201 gctcgacgaa atcgacgagc aggccgatgt tgttcagctc gcgcccccgc gcgtaaatct
     1261 cgacgcggta gttgtggccg tgcaggttct cgcacttgcc cctgtagccg cgcagttggt
     1321 gcgcgctcga aaagttgcgc tcgatcatca cttcgaacat ggctcgaagt ttaaagtcgg
     1381 agtttcaaag tcgcaactcg cggtaacact gtaggggcag ggcttgtcct cggcccgctg
     1441 gtggctcgtt gattcgacgt tgaatcaata cgtcgccgcg ggccgcggac ttgccctgcc
     1501 cctacagtca tcctcgcgtt gacgcttaga ccggctgacg gactttgagc atcgaaggtt
     1561 ggtctttgaa catctcttcc acctcgccga tgtcggtcgt gacgcgcgag ggcgggaggc
     1621 tctggaggaa gaggcgaccg tagccggagg tgcgcaggcg cgggtcgagg acggcgagga
     1681 cgccgcggtc ggtggcgctg cggatgagac gcccgagtcc ctgcttgagg ctgatgatgg
     1741 cctgcgggac gctgtactcg tagaacgagg agccgccctg ctcttcgatg aagcgctggc
     1801 gcgcggagac caccgggtcg gtcggcacgg cgaacgggag cttatcgacg atgacgcacg
     1861 agagctgctg cccgcgcacg tccacgccct gccagaagct cgacgtggcg aacaggacgg
     1921 cgttcggcgt cgcgcggaat ttgtcgagca gcccggcctt cgacatgctg ccctgcacga
     1981 agcaggggaa gtccacttcg cgcgagacgc gctcgtgcag atcgcgcatg ccgctcagag
     2041 aagtcgagag gacgaaggcg cgcccctcgg acgcgttgag gagtttgacg acttcgtcgg
     2101 cggcggcctg cggccactgc ggcgagcgcg gctcgggcat gcggggcgga agataaagca
     2161 gcgcgttcgt ctcgtagtcg tagccggact cggcgatcag ctcctcggtc tcttcgagca
     2221 agcccaaacg accgcggacg aagttgaagc tgcccgcgct cgccagcgtc gcggaggtga
     2281 ggacgaccgt ctcgacgcgc tcgaagagct tgtcttgcag cagcgtggac acgtcgatgg
     2341 gcgaggcgcg caggaacgtg ccgcgcccgc gccgctccgt ccagtagacg tagcgcttgt
     2401 cgtcgcccgc gacgatgaac tccagattga agcgcagctc gcgcacgcgc cgcagcaggt
     2461 tatcgacctc gggcggcttg tccctgagca cgtcgagcgt cgtctccatc cggttgagcg
     2521 cgccgtccag actcaaataa gcctcgccga gcggcgtcgc ctcgatctcg ccctgccggt
     2581 tcttgcgtgc gaacgtgccg ggcacgatgg gcgtgcgccc ctcctcgccg cgcccctcct
     2641 tgaagcccat ccagaactgt tcggcgaagc gcgcgacgcg cgtggacacg cggttcagct
     2701 cgcggttggc gcccacgtcc gtcaggggga gcatctggag gtcgcgcagc acgtcctcga
     2761 cctgatagtt cgacacctgc gcgccgaagt attcggcggc cacgtcctcg acctgatgcg
     2821 cctcgtcgaa gatgacggcg ccgtagtcgg ggatgacctg cccgtactcg ccgccgcgca
     2881 aagccaggtc ggcgaagaag aggtggtggt tgacgatgac gaggtctgcg tccgccgcgc
     2941 gctgccgcat ccgcgtgatg aagcacgcgt cgaagtcggc gcacttctgc ccgaggcagg
     3001 tgtcggagcg cgcgtccacg tgacgccaga aagagaggtt ctccggcagc tcggtcagct
     3061 cggcgcggtc gcccgtctcc gactcgcgcg cccactgccg cacttcgttg aagtagtcga
     3121 tgtcgtccag tccgtcgagg atcggcgtcg tctcggcgcg cttgatgcgg tgcaggcagg
     3181 cgtagttccc gcgccccttc aggtacgcgg cggtgaactt gcgcggcagc acgcgttgca
     3241 ggaaggggat gtccttctcc atcaactgct cttgcaagtt cttcgtgccg gtcgagacga
     3301 cgacgcggca acgtctttcg agcgccgccg cgatggccgg cacgagatac gccagagtct
     3361 tccccgtccc cgtccccgcc tcgacgacga ggtgcttgcg ctcctcgaac gcgcgcagca
     3421 cggccccggc catctcgacc tggccgggcc ggtactcgta gtccgggtgc gcgcgcgcga
     3481 tgaggccgct ggggccgaag atttcttcca tgtctcaagc cgtgcgcgcc cgcgcgcgcc
     3541 tgcggtcgcc gtcgccgcct tgacgtttcg ggcggagttc gaacgagtcg aggaaggggg
     3601 cgccctctgc gttgaccttg acaatcacga caggctccaa cgccgtacga ccgcgcggta
     3661 acgaaaagcc agcggtcagt ataaaggctg cgcctccata ttgaccactg gctgtttctt
     3721 cgcccggcgc gctggggccg ggtcagggac ggcggcggga gcgggcgtgc gcggcaagcg
     3781 caggccgtgc gcccgtccgc cgagcgtgag gccgacgcta ccagttcggg ccgtcgccat
     3841 ccttcatagc gttcggctcg gcgatttcgc cttcctggcc ctgctccgcc gcagaggccc
     3901 ccccctcgtc ctcggacggg tcaaccagct tgctgccgcc gtcgtcttcg gagggatcga
     3961 ccaggttcgc ttcgaattca ttctcttggg gcataacgtt ttctcctttg tcgggttgtt
     4021 caacgtgtcg ccgactggcg agcgtgttta tcacgcaagg tgtcggctgt caaactttta
     4081 agcagtggtc agtggtcggt ggtcggtggt cagtgaaagc gaagaacttt gtttttgctg
     4141 agaaccgaca accgaccact gaccactact tcagtcggcg cccatcgctt cggtttcggg
     4201 ggcgcggcgc acgcgcttcg ggtagagcgg gaagctcgcg gcgagttcgc ggacggagcg
     4261 gcgcacgcgg tcgcgcactt cttcggagtc gggcgcgccg agggcttcgg cgatgaggcg
     4321 cgcgacctcg cgcatctccg cctcgcccat gccgcgcgtg gtcagcgcgg gcgttccgag
     4381 ccgcacgccc gaggcgacga acggcttgtt ggtgtcgaac gggatggtgt tcttgttgac
     4441 cgttatcccg gcggcttcga gcgccttctc ggcgactttg cccgtcacgc cgcggccttc
     4501 catgaacacg tcaaccagga cgaggtggtt gtccgtgccg cccgagacga ggcggaagcc
     4561 ctgct
//
LOCUS       DBNGOH010001028         2709 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_888572, whole genome shotgun sequence.
ACCESSION   DBNGOH010001028 DBNGOH010000000
VERSION     DBNGOH010001028.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2709)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2709)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2709
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_888572"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(172..945)
                     /locus_tag="AC159P_19985"
     CDS             complement(172..945)
                     /locus_tag="AC159P_19985"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017532.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HIR3697778.1"
                     /translation="MTAGLSTQQLERYERDGIVFPVRVFSEGEAARFRGALDAVAERC
                     ARASLKRFDGLHLFFEWAYRLTTHGAVLDAVEGVLGGDILVDGTLVFYKPPQDSGYVS
                     WHQDSVYSDWHLTPTVSAWVALTASRRANGCMRVVPGSHRQGVLDHSNSPDASNLLFR
                     GEQVAEIDEAQAVDVTLRPGEMSLHHSNIVHGSNPNTSDEPRVGFIVRYVTSRVPART
                     RPLLRVRGDADCRHLLLAGPPAKTDPERAFAAWRASEGS"
     gene            complement(1074..2477)
                     /locus_tag="AC159P_19990"
     CDS             complement(1074..2477)
                     /locus_tag="AC159P_19990"
                     /inference="COORDINATES: protein motif:HMM:NF017044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HIR3697779.1"
                     /translation="MNKSNHGSRLIFAALIFLLTSASFASARQTAAPATAPARAAADA
                     QTAKALSDAARLVTEFDVNGLKVLVKRREGSQTVAAGLFIRGGARNITAENAGVEALM
                     LDVATEASTNFPLERMRAELSSMGTVLTYGINYDYSAISLNSTRANFDRSWEIFTDAA
                     LRPSFAPEDFERVKNRIVISRKDDADTPDSYLQQLQAKSAYAGHPYLNDPSGTVESIS
                     RLKIEDVRRYHQQMMQTSRLLIVVVGDLDPEQLRRRVEATFGKLPRGDYRAAALPELS
                     FPAQTVEVTPRALPTNYVQGMFVAPALTAPDIYPMRVASTILQSRVFQEVRVRRNLSY
                     APDAFLRSHGANTGGIYVTAVNANQAVRVMLEEISRLQTTPVSETDLKSTTQHFLTRH
                     YLGQETNAAQAGELAQYELLGGGWRNSAVFLDRLRAVTPQDVQRVAKTYMRNIRFVVL
                     GNPESIDKSAFTNATGE"
BASE COUNT          430 a          826 c          980 g          473 t
ORIGIN      
        1 cccgcgagtc tcgattgttt caatgtcgca tgaacgcgta acagcgggcc gcggacttgc
       61 cctgccccta cagttttgcc gcacgtctcg aatcaattcg aaaagtagtt acagatcgac
      121 ttttaaaagc agttttcaaa atcccgtcag ggactttctt gaactgtttt ttcaggagcc
      181 ttcggacgcg cgccacgcgg cgaaggcccg ttcggggtcg gtcttggcgg gcgggcctgc
      241 gagcaggagg tggcggcagt cggcgtcgcc gcggacgcgg agcagcggtc tcgtgcgggc
      301 ggggacgcgg ctggtgacgt agcggacgat gaagccgacg cgcggctcgt cggaggtgtt
      361 ggggttcgag ccgtggacga tgttgctgtg gtgcagagac atctcgccgg gccgcaaggt
      421 cacatcgacg gcctgcgcct cgtcaatctc ggcgacctgc tcgccgcgga agagcaggtt
      481 ggacgcgtcg ggcgagttcg agtggtcgag cacgccctgc cggtgcgagc cgggcacgac
      541 gcgcatgcag ccgttggcgc ggcggctcgc ggtgagcgcg acccacgcgg agaccgtggg
      601 cgtcagatgc cagtccgagt agacgctgtc ctgatgccaa gagacgtagc ctgagtcctg
      661 cggcggcttg tagaagacga gcgtgccgtc caccagtatg tcgccgccga ggacgccttc
      721 gaccgcgtcg agcacggctc cgtgcgtcgt cagccggtaa gcccactcga agaacaggtg
      781 gaggccgtcg aaccgtttca gcgaagcacg cgcgcagcgc tcggccaccg cgtcgagcgc
      841 gccgcggaag cgggccgcct cgccctccga aaacaccctg accgggaaaa cgatgccgtc
      901 gcgctcgtac cgttcgagct gttgcgtgga gagtccggcg gtcaagacac tgacctcctt
      961 cggaggaggg ggaagaagtc aataagggga agaggggaag agggagaatg agaggcacgc
     1021 tgcttcccgt ccaccttttc gcctcttctc cttttcgcct tttccccctt gtcttattcc
     1081 cccgtggcgt tcgtgaacgc gctcttgtcg atgctctcgg ggttgccgag gacgacgaag
     1141 cggatgttgc gcatgtaggt cttggcgacg cgctgcacgt cctgcggggt gacggcgcgc
     1201 aggcggtcga ggaagacggc ggagttgcgc cagccgccgc cgagcagctc gtactgcgcc
     1261 agctcgccgg cctgcgccgc gttggtctcc tgcccgaggt agtggcgcgt caggaagtgc
     1321 tgcgtcgtgg acttgaggtc ggtctcgctg accggcgtcg tctggaggcg gctgatctct
     1381 tcgagcatga cgcgcacggc ctggttggcg ttgaccgcgg tgacgtagat gccgccggtg
     1441 ttggcgccgt ggctgcgcag gaaggcgtcg ggcgcgtagg agaggttgcg gcgcacgcgc
     1501 acctcctgaa agacgcggct ctggaggatg gtcgaggcga cgcgcatcgg gtagatgtcg
     1561 ggcgcggtca aggccggggc gacgaacatg ccctgcacgt agttggtcgg cagggcgcgc
     1621 ggcgtgacct cgacggtctg cgccgggaag gagagttcgg gcagcgccgc cgcgcggtag
     1681 tcgccgcgcg ggagcttgcc gaaggtggcc tcgacgcggc ggcgcaactg ctctgggtcg
     1741 aggtcgccga cgacgacgat gagcagccgc gaggtctgca tcatctgctg gtggtagcgg
     1801 cgcacgtcct cgatcttgag gcggctgatg gactcgaccg tgccgctcgg gtcgttcaga
     1861 tacgggtggc cggcgtaggc cgacttggcc tgaagctgct gaagataaga gtcgggcgtg
     1921 tcggcgtcgt ccttgcgcga gatgacgatg cggttcttga cgcgctcgaa gtcttcgggc
     1981 gcgaacgagg gccggagggc cgcgtcggtg aagatttccc acgagcggtc gaagttggcg
     2041 cgcgtgctgt tgagcgagat cgccgagtag tcgtagttga tgccgtaggt gagcaccgtg
     2101 cccatgctcg acagctcggc gcgcatccgc tccagcggga agttcgtgct ggcctcggtc
     2161 gccacgtcca acatcagcgc ctcgacgccc gcgttctcgg cggtgatgtt gcgcgcgccg
     2221 ccgcggatga agaggccggc ggcgacggtc tggctcccct cgcgccgctt gaccagcacc
     2281 ttcaggccgt tcacgtcgaa ctccgtgacg agacgggccg cgtcgctcaa agccttggcg
     2341 gtctgcgcgt ccgccgccgc gcgcgcgggc gccgtggcgg gcgccgccgt ctgccgcgcc
     2401 gacgcgaacg aggccgaagt caacaagaag atgagcgccg cgaagatgag ccgtgagccg
     2461 tgattcgatt tattcattgc cgatacgtcc gcgagatatt ccgagcacgt aatccgccgc
     2521 gtagcggcgg ttgaatgtag cccggcgttt caacgccggg gatcaagtaa cgacgggacg
     2581 cagcgtcgcg tcagcgacgc gtgaacccag ctttcaggcg tcgctcacgc gacgcgagcc
     2641 gacaccggcc atacctttcc cggcctttga aaggccgggc tacattcaac cgctgctacg
     2701 cggcggatt
//
LOCUS       DBNGOH010001029         2663 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4371742, whole genome shotgun sequence.
ACCESSION   DBNGOH010001029 DBNGOH010000000
VERSION     DBNGOH010001029.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2663)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2663)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2663
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4371742"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /locus_tag="AC159P_19995"
     CDS             <1..255
                     /locus_tag="AC159P_19995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697780.1"
                     /translation="RLDPDAAVDDDDLPEADAADAAPRFPQLWQVAACLCLLAAAALW
                     LLGHADAAFVAAALGVIAWFLNVRVQLRRKNNHSDTEARR"
     gene            complement(369..617)
                     /locus_tag="AC159P_20000"
     CDS             complement(369..617)
                     /locus_tag="AC159P_20000"
                     /inference="COORDINATES: protein motif:HMM:NF024139.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR3697781.1"
                     /translation="MDGAREDFLTARQLASIMQVSESTVRRLAREGRIPSVRVTPRIL
                     RFNLKAVRHALDGHTRPRADRQAPHDDPQLSFDDLLKV"
     gene            complement(658..1320)
                     /locus_tag="AC159P_20005"
     CDS             complement(658..1320)
                     /locus_tag="AC159P_20005"
                     /inference="COORDINATES: protein motif:HMM:NF025006.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MSCRAMM family protein"
                     /protein_id="HIR3697782.1"
                     /translation="MLYRKSNTLRVTCLLFACALGAAPVHAQKNKQAPPQPTTGSIRG
                     RVRVGGDNTPADIAITVRQGEREVARLATTVQGDFEVNNLEPGIYGLTLRKAGLQTGR
                     MEELKVSAGKVVNLKDRLYLAVDEGSIAFLKGSVFSKAGRSLGGVRVEVVLLRADGSE
                     KKINSAVTNSLGSFSFRLPPAPARYRVTAKASGLETTSKDVQIEGASIHRVALELGPA
                     AK"
     gene            1544..2617
                     /locus_tag="AC159P_20010"
     CDS             1544..2617
                     /locus_tag="AC159P_20010"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HIR3697783.1"
                     /translation="MSTPSTSPTPRKPPARRPVLFVLFCAALSLMAAATFAQPADAPP
                     RQQGTRGTPVEVTVGEDDVLKIDTDLVLVDVTVTDAEGRPVRGLKAEDFKLYEDGEER
                     EVAFLNVERRGGAERPVAVVFAVDISGSMTSEEILRLRAAMQAFSEKLAGRPASFALM
                     SFGMGAKVHQSFTSDPRKLARSLERLARDVDGLSTHTYDAVDDAIRLLVRDAPRTRER
                     RLMKRAVVVITDGFPVGDTVSPKTVIERANAAEVSVYTVTLPSNSQLISPAAARRAPL
                     PTPLDVSGLADKTGGTGVYAHEKDYEPLFKSLAEEVSSTYVLAYYPPEEKRRDGRLHT
                     IRVEAPRGLRVRQNRTIYKSRDR"
BASE COUNT          392 a          928 c          909 g          434 t
ORIGIN      
        1 cgcctcgacc ccgacgcggc ggtggacgac gacgacttgc ccgaagctga tgccgccgac
       61 gcggcgccgc gcttcccgca actgtggcag gtcgcggcct gcctctgcct gctcgccgcg
      121 gcggccctct ggctgctcgg ccacgccgac gcggccttcg tcgccgccgc gctcggcgtc
      181 atcgcatggt tcctcaacgt gcgcgtccaa ctccggcgaa agaataacca cagcgacacg
      241 gaggcacgga gataaaagaa aggcccggcg gagcagacgc gtcgaggggg agtcgagcgg
      301 ttcgactccc cctcgacggt caacgcatct tccgggtctt tatctacgtt cctctgcgcc
      361 cctgtggctt aaaccttcag caggtcgtcg aaggagagtt gcgggtcgtc gtgcggcgct
      421 tggcggtctg cgcgcgggcg cgtgtggccg tcgagggcgt ggcgcacggc cttgaggttg
      481 aagcggagga tgcgcggcgt gacgcggacg gaggggatgc gcccctcgcg cgcgaggcgg
      541 cgcacggtcg attcgctcac ctgcatgatg gaggcgagct ggcgcgccgt caggaaatct
      601 tcgcgcgccc cgtccatgtg agacgcatct ttcactttat tttttcgcgg gccgctgcta
      661 cttcgcggcg gggccgagtt cgagcgcgac gcggtggatg gacgcgccct cgatctggac
      721 atccttcgat gtcgtctcca gcccgctcgc cttcgccgtc acgcggtagc gcgcgggcgc
      781 gggcggcagc cggaacgaga acgagccgag gctgttggtg acggcgctgt tgatcttctt
      841 ctccgagccg tcggcgcgca gcagcacgac ctcgacgcgg acgccgccca gactccgccc
      901 ggccttgctg aacacgctcc ccttgaggaa cgcaatcgag ccttcgtcca ccgcgaggta
      961 cagcctatcc ttcaggttga cgaccttgcc cgcgctcacc ttcagctctt ccatcctgcc
     1021 ggtctgcaac cccgccttgc gtagcgtcag accgtagatg ccgggttcga ggttgttgac
     1081 ctcgaagtcg ccctgcacgg tggtcgcgag gcgcgcgacc tcgcgctcgc cctggcggac
     1141 ggtgatggcg atgtccgctg gggtgttgtc gccgccgacg cggacgcggc ccctgatgct
     1201 gcccgtcgtg ggctgcggcg gcgcctgctt gttcttctgc gcgtggacgg gggcggcgcc
     1261 gagcgcgcac gcgaagagca gacaggtgac gcggagggtg ttcgactttc gataaagcat
     1321 ttcgttctcg ccggaaagtg tgtgcgccgc cgtgcgaccg ggcggcggtg attataccac
     1381 gcgggcttcg cgcgcgggcg gtcgcgattc ggcgcgcgcc ttgaattgat gagcggaggg
     1441 ggccgcgtgt tgcccggcga aagggtctgg cgcgcgcggc ccggcgtcgg tataatccgt
     1501 cgcacctccc gcgcggtagt ctgagacgtt ctacgaaagc ggtatgtcaa ccccttccac
     1561 ttcgccaacg ccacgcaagc cccccgcccg gcggcccgtc ctcttcgtcc tcttctgcgc
     1621 ggcgctgtct ctgatggcgg cggccacgtt cgcgcagccc gcggacgccc cgccgcggca
     1681 gcaagggacg cgcggcactc ccgtcgaggt gacggtcggc gaggacgacg tgctgaagat
     1741 cgacaccgac ctcgtgctcg tggacgtgac ggtgacggac gcggaagggc ggcccgtgcg
     1801 gggtctgaag gccgaagact tcaagctcta cgaggacggc gaggagcgcg aggtcgcctt
     1861 cctcaacgtc gagcggcgcg gcggggcgga gcgcccggtg gccgtcgtct tcgccgtgga
     1921 catttccggc agcatgactt cggaggagat tctgcggctg cgcgcggcga tgcaggcctt
     1981 ctccgaaaaa ctcgcggggc ggcccgcctc gttcgcgctg atgagcttcg gcatgggcgc
     2041 caaagtccac cagagtttca ccagcgaccc gcgcaagctc gcgcgctcgc tcgaacggct
     2101 cgcccgcgac gtggacggac tctcgacgca cacctacgac gccgtggacg acgccatccg
     2161 cctgctggtg cgcgacgcgc cgcgcacgcg cgagcggcgc ctgatgaagc gcgccgtggt
     2221 cgtcatcacc gacggctttc cggtcggcga cacggtctcg cccaaaaccg tcatcgagcg
     2281 cgccaacgcc gccgaggtca gcgtctacac cgtgacgctc ccctccaact cgcaactgat
     2341 ctcgccggcg gcggcgcggc gcgccccgct gccgacgccg ctcgacgtga gcgggctggc
     2401 ggacaagacc ggcggcaccg gcgtctacgc ccacgagaag gactacgagc cgctcttcaa
     2461 gtcgctggcc gaggaggtct cctcgaccta cgtgctcgcc tactacccgc ccgaagaaaa
     2521 aaggcgcgac gggcgcctcc acaccatccg cgtcgaagcc ccgcgcggcc tccgcgtgcg
     2581 ccagaatcgc accatttata agagccgtga ccggtaaagc cgttagcgat cagcttcgag
     2641 ccgtttcacc tcgatctcgc cga
//
LOCUS       DBNGOH010001030         2471 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2355566, whole genome shotgun sequence.
ACCESSION   DBNGOH010001030 DBNGOH010000000
VERSION     DBNGOH010001030.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2471)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2471)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2471
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2355566"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..539
                     /locus_tag="AC159P_20015"
     CDS             <1..539
                     /locus_tag="AC159P_20015"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013563208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HIR3697784.1"
                     /translation="PDAKTHSPIKRGDFVLFDVWAKLKKPGAVYADQTWVGYVGETVP
                     DEYTRVFRLVREARDAATDFVRSAAREGRAIRAAQVDDVARQIIARAGYGEQFTHRTG
                     HSIGEEVHGNGAHIDNLETRDSRRIIPRTSFSIEPGIYLEGKFGVRSEINLYVTDKEI
                     EVTGQPIQTEIVPILKDQ"
     gene            617..1387
                     /locus_tag="AC159P_20020"
     CDS             617..1387
                     /locus_tag="AC159P_20020"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014101520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HIR3697785.1"
                     /translation="MLNFAIQTARDAGRVLAEKFGRVTITHKGDIDIVTEADLAAERL
                     IVERVRSYHPRHAVLAEEAGEVIAVGGGDAEYKWIVDPLDGTTNYAHGYPVFCVSIAL
                     EHKGEIVVGVVYDPLRDEVFAAERGEGATLNGRRMRVSDTSVLNRALLCTGFPYDVRR
                     RSQFARHFAAFIMHAQSVRRDGAAALDLAYVAAGRLEGFWEEGLRPWDVAAGTLLVRE
                     AGGRVTHYDGTPFRVYTPPILATNGLVHEAMMEVLQEP"
     gene            1658..2134
                     /locus_tag="AC159P_20025"
     CDS             1658..2134
                     /locus_tag="AC159P_20025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697786.1"
                     /translation="MRKPVSKIFFVVAAACAVFTFAADADAQRRFRTPRERGYTRAEV
                     NAVIRRVETRTDEFVKLFDKSLDRSGLDGTRREDNLNDRAKDLEKATDDLRREFDRKE
                     SYVATRPEVARCLNIAGNIDNTMRRRRLGGQTERQWALLRAELNELARLYNLPRVR"
BASE COUNT          450 a          858 c          759 g          404 t
ORIGIN      
        1 agcccgacgc caaaacacac tcgcccatca agcgcggcga cttcgtcctc ttcgacgtgt
       61 gggccaaact gaaaaagccc ggcgcggtct acgccgacca gacgtgggtc ggctacgtcg
      121 gcgagaccgt gcccgacgaa tacacacggg tcttccgtct cgtccgcgaa gcgcgcgacg
      181 ccgccaccga cttcgtccgc tccgccgccc gcgaaggccg tgccatccgc gccgcgcagg
      241 ttgacgacgt ggcgcgccaa atcatcgcgc gcgccggcta cggcgagcag ttcacgcacc
      301 gcaccggcca ctccatcggc gaggaagtcc acggcaacgg cgcccacatc gacaacctcg
      361 aaacccgcga ctcgcgccgc atcatccccc gcacctcgtt ctccatcgaa cccggcatct
      421 acctcgaagg caaattcggc gtccgctccg aaatcaacct ctacgtcacg gacaaagaaa
      481 tcgaagtcac cggccagccc atccagaccg agatcgtccc catcctcaaa gaccagtagg
      541 cagtaggcag taggcggtag agaggcggcc ttgcttttct accggctact ggctactgtc
      601 tactgactac ttctttatgc ttaacttcgc catccagacc gcacgcgacg cgggacgcgt
      661 gctcgcggag aagttcgggc gcgtgacgat cacgcacaag ggcgacatcg acatcgtcac
      721 ggaggccgac ctcgccgccg agcggctgat cgtcgagcgc gtgcgcagct atcacccgcg
      781 ccacgcggtg ctggccgagg aggcgggcga ggtcatcgcc gtgggcggcg gggatgccga
      841 gtacaagtgg atcgttgacc cgctcgacgg gacgaccaac tacgcgcacg gctacccggt
      901 cttctgcgtc tccatcgcgc tcgaacacaa aggggagatc gtggtcggcg tcgtctacga
      961 ccccttgcgc gacgaggtct tcgccgccga gcgcggagag ggcgcgacgc tcaacggtcg
     1021 ccgcatgcgc gtctcggaca cgagcgtgct gaaccgcgcg ctgctctgca ccggcttccc
     1081 ctacgacgtg cgccggcgct cgcagttcgc gcgccacttc gccgccttca tcatgcacgc
     1141 acagtccgtg cggcgcgacg gcgccgccgc gctcgacctc gcctacgtcg ccgccgggcg
     1201 cttagagggc ttctgggaag agggcctgcg cccctgggac gtggccgccg gcaccctcct
     1261 cgtccgcgag gccggcggac gcgtcaccca ctacgacggc acccccttcc gcgtctacac
     1321 cccgcccatc ctcgccacca acggcctcgt ccacgaagcc atgatggaag tgctccaaga
     1381 gccgtgaacc gtgaaccgtc aaccgtgata agagagaaac tgcttttcac ttgtcacggt
     1441 tgacggttca ctcattacgg cttttcactc aacctctttt cacttatcac ggttgacggt
     1501 tcacggttca cggctttttc tcggccagcg atgcggggcg cgcgtctaaa gggacggtgg
     1561 cgcgcgcgtg cgcgcacgtt cttcgagccg tcgggagatg ggtgcggctt ctctctcgcg
     1621 aaagcgtgcc gcgccgggag aacttagagg agagagtatg agaaaaccgg tgtcgaaaat
     1681 tttcttcgtc gtggccgccg cgtgcgccgt cttcacgttc gcggcggacg ccgacgcgca
     1741 gcggcgcttc cgcactccgc gcgagcgcgg ctacacgagg gccgaggtga acgcggtcat
     1801 ccgccgcgtc gagacgcgca cggacgagtt cgtgaagctg ttcgacaagt cgctcgaccg
     1861 cagcgggctg gacggcacgc ggcgcgagga caacctgaac gaccgcgcga aggatttgga
     1921 gaaggcgacc gacgacttgc gccgcgagtt cgaccgcaag gagagctacg tcgccacgcg
     1981 gcccgaagtc gcgcgctgcc tgaacatcgc cggcaacatc gataacacga tgcgccgccg
     2041 ccgcctgggc gggcagaccg agcggcagtg ggcgctcctg cgcgccgagc tgaacgagct
     2101 ggcgcgcctc tacaacctgc cgcgcgtgcg gtgaggaaaa gcaacgggca gcaagcagca
     2161 ggcagtcaaa gcaagaggct tgagatgcaa cgtctcaagc ctcttcattc ttctgacttc
     2221 tgacttctgt ctcctgactt cttctgttct tactgcctgc tgcctgctgc cttctgcctt
     2281 ctgctatatt gtcgcgaggc cgcacgtcga aggagagacg gatgatcgca cccggcacga
     2341 ttttgcagaa ccgctacctg gtcgaggagc agatcgggca gggcgggatg ggcgcggtct
     2401 tcgtcgccac cgaccagcgc ttcggcagcc gcgtcgcgct caaagagacc ttcttcacag
     2461 accccaacct g
//
LOCUS       DBNGOH010001031         1890 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1257199, whole genome shotgun sequence.
ACCESSION   DBNGOH010001031 DBNGOH010000000
VERSION     DBNGOH010001031.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1890)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1890)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1890
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1257199"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..263)
                     /locus_tag="AC159P_20030"
     CDS             complement(<1..263)
                     /locus_tag="AC159P_20030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697787.1"
                     /translation="MRRISSLLLLVVLAVWCASPAAAQGGAARTPAAGSAERRAILDA
                     LRAPVERELKRKVVFKVDHLKVQGAWAFMRGVPQQPDGRPMDYR"
     gene            complement(371..1003)
                     /locus_tag="AC159P_20035"
     CDS             complement(371..1003)
                     /locus_tag="AC159P_20035"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HIR3697788.1"
                     /translation="MVERLTAEGNDEHLVKAGQNTTGWVEVIAGSMFSGKSEELIRRL
                     RRAKIARLKVQVFKPEIDLRYSRDHIVSHSEMRHESNAVHSAREILELLDPATEVVGI
                     DEGQFFDNDLVEIVNQLAGRGVRVIVAGLDQDYTGRPFEPMPQLLAVAEYITKTHAIC
                     VRCGQPANYSQRIVEVEGRVVVGAADAYEARCRRCFVPHADAPTRYIEGG"
     gene            complement(1031..1597)
                     /locus_tag="AC159P_20040"
     CDS             complement(1031..1597)
                     /locus_tag="AC159P_20040"
                     /inference="COORDINATES: protein motif:HMM:NF014041.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF84 family protein"
                     /protein_id="HIR3697789.1"
                     /translation="MLIALGSARPAKIMALRAACARVAEVDRRWKQAELVTRGVETGV
                     ARMPLNDTQLMRGARNRAEAVRELLSSEGSPADFYVGLEGGFHSITFDGERRTFLHGW
                     AYVSDGARGYFGHAPAVTVPARIVARVEQTRRELGEVIDEVAGQTDVRSRQGAWGVLS
                     RGLLTRAMSFETALVAAFAPFYNARLYE"
BASE COUNT          308 a          641 c          645 g          296 t
ORIGIN      
        1 cggtagtcca tcgggcggcc gtcgggctgc tgcggcacgc cgcgcatgaa ggcccacgcg
       61 ccctgcacct tgaggtggtc aaccttgaag acgaccttcc gcttcagctc ccgctcgacg
      121 ggcgcgcgca gcgcgtcgag gatggcccgg cgctcggccg acccggccgc gggcgtccgc
      181 gccgccccgc cctgcgccgc ggccggcgac gcgcaccaca cggcgaggac gacgagcaag
      241 agcagcgacg agattcttcg cacgtgttga aaccccccga gcagaatttt cgattttcga
      301 ttttcgattg gcgattcgga ggcagggtgc gggggtgctc ttccaatcaa aaatcaaaaa
      361 ttcatatccc ctatccccct tcgatgtagc gcgtgggcgc gtcggcgtgg gggacgaagc
      421 agcggcggca gcgggcctcg taagcgtccg cggcgccgac gacgacgcgc ccttcgacct
      481 cgacgatgcg ctgcgagtag ttggcgggct ggccgcagcg gacgcagatg gcgtgcgtct
      541 tggtgatgta ctcggcgacg gcgagcagtt gcggcatcgg ctcgaagggg cgccccgtgt
      601 agtcctggtc gagccccgcg acgatgacgc gcacgccgcg cccggcgagc tggttgacga
      661 tctccacgag gtcgttgtcg aagaactgcc cctcgtcgat gccgacgacc tcggtggcag
      721 ggtcgagcag ctccaggatc tcgcgcgcgc tgtgcacggc gttcgactcg tggcgcatct
      781 cggagtgcga gacgatgtgg tcgcgcgagt agcgcaggtc gatctcgggc ttgaagacct
      841 gcaccttgag gcgcgcgatc ttcgcgcggc gcagacgccg gatcagctcc tccgacttgc
      901 ccgagaacat cgagccggcg atgacttcga cccagcccgt cgtgttctgc cccgccttga
      961 cgaggtgctc gtcgttacct tctgcggtga gtctctcgac catccttctc tcagaacctt
     1021 tccccaaatc ttactcgtag aggcgcgcgt tgtagaacgg cgcgaaggca gcgacgagcg
     1081 ccgtctcgaa gctcatcgcg cgggtcaaca ggccccgcga gagcacgccc cacgcgccct
     1141 gccggctccg cacgtccgtc tgcccggcga cctcgtcgat gacctcgccc agctcgcggc
     1201 gcgtctgctc gacgcgcgcg acgatgcggg cggggacggt gacggccggc gcgtggccga
     1261 agtagccgcg cgcgccgtcc gagacatagg cccagccgtg caggaacgtc cgccgctcgc
     1321 cgtcgaaggt gatggagtgg aaaccgcctt cgagcccgac gtagaagtcg gcggggctgc
     1381 cctccgagga gagcagctcg cggacggcct cggcgcggtt gcgcgcgccg cgcatgagct
     1441 gcgtgtcgtt gagcggcatc cgcgccacgc ccgtctccac gccgcgcgtg accagctccg
     1501 cctgcttcca gcgtcggtcg acctccgcga cgcgcgcgca ggccgcgcgc agcgccatga
     1561 tcttggccgg gcgcgccgag ccgagtgcga tcagcatcct tatcaccttt ggcgccgagg
     1621 ggccttgcgg ggagtctgcg gccggagggc gcggggcgcg acgaggctat aacaggaggg
     1681 gtggaaaagg caaaagcggc cgccgccggg gcgcccggga aaaatatttc ttgacagaaa
     1741 tcgcgcgcgg ggatattttg tccaaacttc gaacaaatgc ccggcggcag tccatgagaa
     1801 aaatcaacac gcacgatttc aaggtggcca cgcggacgac ctcgcgtgat atcaaccgcc
     1861 gcatcgcgct caacctgatt cgcgagcacc
//
LOCUS       DBNGOH010001032         8818 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1989932, whole genome shotgun sequence.
ACCESSION   DBNGOH010001032 DBNGOH010000000
VERSION     DBNGOH010001032.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8818)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8818)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8818
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1989932"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..461
                     /locus_tag="AC159P_20045"
     CDS             <1..461
                     /locus_tag="AC159P_20045"
                     /inference="COORDINATES: protein motif:HMM:NF017044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HIR3697790.1"
                     /translation="TLSSRLGLRVRDAEGLTYGIASRFRSPSLAAGPWYIAVSVNPSN
                     VERALASALAVLRDYVEHGIRPEELEDEKSAATGAFKVSLSTNAGLASALWNAEFYRL
                     GLDYIDRYPELIRAVTVEEVNAAIRKYFRPDHLTVVIAGDVETAHAEAIA"
     gene            complement(657..3452)
                     /locus_tag="AC159P_20050"
     CDS             complement(657..3452)
                     /locus_tag="AC159P_20050"
                     /inference="COORDINATES: protein motif:HMM:TIGR03118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03118 family protein"
                     /protein_id="HIR3697791.1"
                     /translation="MSHPRPVKRLVVRWAAVALAYALAVAVVPSLPASVAGPAGGAAS
                     AQEPQLPQIPVIAPGSAYRQTNLLSDVPGLAPVLDPLLVNPWGLTVRGTSPFWVANNG
                     TSTTQLIRSATGGGDPIVLNPNPQTINIPGGLPTGTVGNSTTDFRITPPGGGTPAAAN
                     FIFDSITGNITAWNGASGAAAQNVVSMPGHVWTGLAIGANAGGNRLYAADFANKHIDV
                     FNGTFTATTVTGGFVDATIPASYAPYNIQNLGGSLYVAYAQVGMDGLPVDGVGNGFVR
                     KFNTDGVRDLTFAINNGALNAPWGLAIASANFGIFSNALLVGNFGRGNPSIHAYNPTT
                     GAFLGTLQNEDGFGIEIDELWALQFGNGGNGGDVNTLYFTAGVGNEEHGLFGKLNPTT
                     ASATSLIEFATDSFSVDEAGKFVDFTVIRTGDTAGTATVNYNTFDESQAGHASQKSDY
                     EIALGTLSFAAGETSKTFRILVVDDKFDESDEVIELYLSNPTGTGVGLGSPNTAVINI
                     LDNDTGAPASNPIDTASFFVRQHYLDFLNREPDAAGLAFWTNQMTNCSNPPPADLTVC
                     KVNVSAAFFLSLEFQRTGYLAYLTHRAAFGANASGSPAPVLYGNFQRDTQALQRGFIF
                     GQPGSDAVLEANKVAYFNEFVTRPEFVAKYPSTQTNAEFVDALLVSANLSPNDFIVNM
                     TNAQEPPPLNPTTTGGVRRPASYGTARIQVNAAQTALTITVTVNNIDFTGSQTADTND
                     NLTNAHIHASAVEPPPATRPVVFGFIGTPFNDNNPNDAVVTPFGSGVGGTVSSKWDPP
                     EGQNTTLAAQLPNIFAGRAYINFHTVQFGGGEVRGNFPTLTAFRDSLVTGLNNMTLTR
                     ATVLRMVAESQELNTREFNRAFVTMEYFGYLRRDPDTGGFNFWLDKLNSFNGNFQDAE
                     MVRAFIESLEYRQRFGP"
     gene            complement(3969..4991)
                     /locus_tag="AC159P_20055"
     CDS             complement(3969..4991)
                     /locus_tag="AC159P_20055"
                     /EC_number="1.1.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045242 - isocitrate dehydrogenase
                     complex (NAD+) [Evidence IEA];
                     GO_function: GO:0004449 - isocitrate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006102 - isocitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate dehydrogenase (NAD(+))"
                     /protein_id="HIR3697792.1"
                     /translation="MKHTITLIPGDGIGPEVSSAVVRIIEASGAEVQWETHYAGAQAL
                     EKFGNTLPEDLLESIGRNKVALKGPITTPVGKGFTSVNVGLRKKLDLYANLRPVRALP
                     NVPCRYPELDLVVVRENTESLYAGLEHVVVPGVVESIKIITEKASTRIARYAFEYARR
                     EGRKKVTAVHKANIMKLSDGLFLECFRNVSKDYPEIEADDKIVDNACMQLVMRPEQFD
                     IMLLENLYGDIVSDLCAGLVGGLGLVPGANIGEQGAVFEAVHGSAPDIAGQGIANPTA
                     LLQSGILMLRHLGERGPADRIEQSMLKVFTEGRVRTRDIGGTASTYEFADAIIAQMAQ
                     QGGAAA"
     gene            5154..6359
                     /gene="thiO"
                     /locus_tag="AC159P_20060"
     CDS             5154..6359
                     /gene="thiO"
                     /locus_tag="AC159P_20060"
                     /EC_number="1.4.3.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016647 - oxidoreductase activity, acting
                     on the CH-NH group of donors, oxygen as acceptor [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine oxidase ThiO"
                     /protein_id="HIR3697793.1"
                     /translation="MRNEWDSGAADVVVVGGGVMGLAAARALARKGSSVVLVERGRVG
                     AEASWAAAGMLAPQAEADRRDEFFDLLCAGRDLYPAFADALRAETGIDIELDRTGTLY
                     LALTESDEHEVLRRYEWQSRAGLPVERLTAEEARACEPSVAENVRAALRFPADWQVEN
                     RRLVAALAASAASFGVRLLTGVEVTSILVERGRARGVETSRGTLAAGAVVVAAGAWSS
                     LIRFERVGRGAQGEDREDAAACPRVEPVRGQMLCFDARPRVVRHVVYSPRGYVVPRRD
                     GRLLAGSTTEHVGYDKAVTGEGLRAIAAGAQEIAPRVGGLALVDSWSGLRPRAADDWP
                     VLGASEEVERLYYATGHYRNGILLAPLTGEVVAGLVTGGEALINGRAREAFAPGRFLR
                     AAVGSRGGR"
     gene            6369..6689
                     /locus_tag="AC159P_20065"
     CDS             6369..6689
                     /locus_tag="AC159P_20065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697794.1"
                     /translation="MYLFTIRSRINPKSKAAAKHEDVGGAYVNCYIQFKDYEAAEKLA
                     RLLIRESGWIPEKRTEASVIQKREMKTKKDKQFYSETLKYGYTLVFHTWPKDAEDADV
                     DYEE"
     gene            6697..8055
                     /locus_tag="AC159P_20070"
     CDS             6697..8055
                     /locus_tag="AC159P_20070"
                     /inference="COORDINATES: protein motif:HMM:NF012787.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HIR3697795.1"
                     /translation="MKSFILHRAAFIIAATALVFNTSPGIETAAQSNTQTEAGANGGG
                     GAATASTAQALYEEAESYVRRKFDEFAKKNIPYSRNLEAVTYQEQRELALKHAGTIAA
                     RKGELRGPDLYHAGLLYALAGKPEQALDALRRFLAEDAGAAPALRQKARVVFVQHAVE
                     KDLAAEAEKVLADYAREEPRAPAEAHRMNSALATYYHKKKDYERAAARAREAYGITLG
                     LARGKTLGARQRADTVYGTGAFLVDALVKSKQRAEALGVIQQMRSLSLELPSARLYGN
                     ATVLLLDNGEPLAAPPLGDASTATAPPEIKVKHWIDQPPASLADLRGRVVLLDFWATW
                     CGPCRFTIPKLNALHKKYNARGLTIIGLTEFYGNGEGKELSPPEELAYLRRFKREREI
                     AYGFGVADDKENDTSYGVAQMPTAVLLDRRGRVRFITVGASDIEARALAEMVEKLIDE
                     TP"
     gene            8209..8697
                     /locus_tag="AC159P_20075"
     CDS             8209..8697
                     /locus_tag="AC159P_20075"
                     /inference="COORDINATES: protein motif:HMM:NF015863.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HIR3697796.1"
                     /translation="MRSFIYRAHTWLGLAVALPVLAWASSGLLYAWPGAVEGGKVEVI
                     DTARVRLSAPEAVARANEFAGRRLPTTALTLLMRDNRPYYQSVGGMGADSLLIDAETG
                     AVLKTPPPNLKTRYFRQAHFYFFAGAWQVPLLILLSALACLSAASGVYLNLTMWRGRK
                     KR"
BASE COUNT         1418 a         2855 c         3052 g         1493 t
ORIGIN      
        1 cgacgctctc gtcgcgcctg ggcctgcgcg tgcgcgacgc cgaggggctg acctacggca
       61 tcgcgtcacg cttccgctcg ccgagtctgg cggccgggcc gtggtacatc gccgtctcgg
      121 tcaacccttc gaacgtcgag cgcgcgctcg cctcggcgct cgcggtcttg cgcgactacg
      181 tcgagcacgg catccgcccc gaagagctgg aagacgagaa gtcggcggcc acgggggcgt
      241 tcaaggtctc gctctcgacg aacgcgggac tggcctccgc gctctggaac gcggagttct
      301 accgcctcgg cctcgactac atcgaccgct acccggagct gatccgcgcc gtcacggtcg
      361 aagaggtcaa cgccgccatc cgcaaatact tccgcccaga ccacctcacg gtcgtcatcg
      421 cgggcgacgt cgagaccgcg cacgcagagg ccattgcttg aagcggtagg cagtaggcgg
      481 taggcggtag gcagttaaag cagaagggtg aagaggctcg ggaggtaaag tcccgagcct
      541 ctctcttctt ccgtcaagca agaaagcaag aggcttgaga cgctagctct caagcctctt
      601 gcccttgctc ttgctttaac tgcctactgc ctactgccta ctgcctcacg gctccctcac
      661 gggccgaagc gctggcggta ctcaagcgac tcgatgaagg cgcgcaccat ctcggcgtcc
      721 tggaagttgc cgttgaagga gttgagcttg tcgagccaga agttgaagcc gccggtgtcg
      781 gggtcgcggc gcagatagcc gaagtactcc atcgtgacga aggcgcggtt gaactcgcgc
      841 gtgttcagct cctgcgactc ggccaccatg cggagcacgg tggcgcgcgt cagcgtcatg
      901 ttgttcaggc cggtgacgag cgagtcgcgg aaggcggtga gcgtcgggaa gttgccgcgc
      961 acctcgccgc cgccgaactg caccgtgtgg aagttgatgt aggcgcgccc cgcgaagatg
     1021 ttcgggagct gcgcggcgag cgtcgtgttc tgcccctcgg gcgggtccca cttggagctg
     1081 acggtgccgc cgacgccgga gccgaagggc gtcaccaccg cgtcgttggg gttgttgtcg
     1141 ttgaagggcg tgccgatgaa gccgaagacg acgggtcggg tcgccggagg cggctcgacc
     1201 gcgctggcgt ggatgtgcgc gttggtcagg ttgtcgttgg tgtcggcggt ctgcgagccg
     1261 gtgaagtcga tgttgttgac cgtcaccgta atcgtcagcg ccgtctgggc ggcgttgacc
     1321 tggatgcgcg ccgtgccgta ggaggccggt cggcgcacgc cccccgtggt cgtcgggttg
     1381 agcggcggcg gctcctgcgc gttcgtcatg ttgacgatga agtcgttcgg cgacaggttc
     1441 gccgacacga gcagcgcgtc cacgaactcc gcgttcgtct gcgtcgaggg gtacttggcg
     1501 acgaactcgg ggcgcgtgac gaactcgttg aagtaggcga ccttgttcgc ttcgagcacg
     1561 gcgtccgacc cgggctggcc gaagatgaag ccgcgctgga gcgcctgcgt gtcgcgctgg
     1621 aagttgccgt agagcacggg cgcggggctc ccggacgcgt tcgcgccgaa ggccgcgcgg
     1681 tgcgtcaggt aggcgaggta gcccgtgcgc tggaactcca gcgacaggaa gaaggccgcc
     1741 gagacgttga ccttgcagac agtcaggtcg gcgggcggcg ggttggagca gttcgtcatc
     1801 tggttcgtcc agaaggctaa cccagcggcg tccggctcgc ggttgaggaa gtcgaggtag
     1861 tgctggcgga cgaagaacga agcggtgtcg atggggttcg acgccggcgc ccccgtgtcg
     1921 ttgtcgagga tgtttatcac agccgtgttc gggctgccca gcccgacgcc cgtccccgtc
     1981 gggttcgaga ggtagagttc gatcacctcg tccgactcgt cgaacttgtc gtccacgacc
     2041 aggatgcgga acgtcttcga ggtctcgccc gcggcgaagc tgagcgtgcc gagcgcgatc
     2101 tcgtagtccg acttctgcga ggcgtggccg gcctgcgact cgtcgaaggt gttgtagttg
     2161 acggtggccg tgcccgcggt gtcgcccgtg cggatgacgg tgaagtcaac gaacttgccg
     2221 gcctcgtcca cggagaagct gtcggtggcg aactcgatga gcgaggtggc gctggcggtc
     2281 gtcgggttga gcttgccgaa gaggccgtgc tcctcgttgc cgacgccagc ggtgaagtag
     2341 agcgtgttca cgtcgccgcc gttgccgccg ttaccgaact gcaaggccca cagctcgtca
     2401 atctcgatgc cgaagccgtc ctcgttctgg agcgtgccga ggaaggcgcc ggtcgtcggg
     2461 ttgtaggcgt ggatcgaggg gttgccgcgc ccgaagttgc cgacgagcag ggcgttgctg
     2521 aagatgccga agttggcgga cgcgatggcc aagccccacg gcgcgttgag cgcgccgttg
     2581 ttgatggcga aggtcaggtc gcggacgccg tcggtgttga acttgcggac gaagccgttg
     2641 ccgacgccgt ccacggggag gccatccatc ccgacctgcg cataggcgac gtagagcgag
     2701 ccgccgaggt tctggatgtt gtagggggcg tagctcgcgg ggatggtcgc atcgacgaag
     2761 ccgcccgtga cggtcgtcgc cgtgaaggtg ccgttgaaaa cgtcgatgtg tttgttggcg
     2821 aagtcggcgg cgtagagccg gttgccgccg gcgttagcgc cgatggccag ccccgtccag
     2881 acgtgccccg gcatcgagac gacgttctgc gcggcggcgc cgctcgcccc gttccaggcg
     2941 gtgatgttgc cggtgatcga gtcgaagatg aagttggccg cggcgggcgt gccgccgccc
     3001 ggcggggtga tccggaagtc ggtggtcgag ttgccgaccg tccccgtggg aagcccgccg
     3061 gggatgttta tcgtctgcgg gttcgggttg aggacgatgg ggtcgccacc gcccgtggcg
     3121 ctgcggatga gctgcgtcgt gctcgtcccg ttgttggcga cccagaaggg gctggtgcct
     3181 ctgaccgtga ggccccacgg gttgacgagc aggggatcga ggacgggcgc gaggccgggc
     3241 acgtccgaga gaaggttggt ctgccggtag gcgctgcccg gcgcgatgac cggaatctgc
     3301 gggagctgcg gctcctgcgc gctggccgcg ccccccgccg ggccggcgac ggacgcgggc
     3361 agtgagggca cgacggcgac ggcgagcgcg taggccagcg cgacggcggc ccaacgcacg
     3421 acgagacgtt tcacgggacg tgggtgcgac atgacttatc ctcctacggg aatcgaaaga
     3481 ttgttgtccg aagaggtcgg gcgaaggccg accggtcgcc gaggcgcggg ttgcgacgcg
     3541 gcgggatgaa gacaacggcg tcgtgtgcga ggcgagccgc ggggcgggga gacagacggt
     3601 gccggcgcgg gtcacttgac ccgaggcccc gcctgcgacc ggtaaggcgc gggcgggctg
     3661 acgacggggc cttgatccga aaacatcttt tcaggaagcg cggaaaactc tattccgcac
     3721 cgcccacgct gtcaaggttt ttcggcgccg agcctcggcc ctcgtccgtt cgttctccgg
     3781 acggaacacc tcggccaccg cagacgcggc gagcgaaaac aaggatggag aaaacgcgtc
     3841 gagggagagc tgaatcattc aaccctccct cgtgcccggt ctcttcctgc ctctgttccg
     3901 attcttctct cggtgtctgc ggcggccctt cggctgaacg cttttgcgaa cgcgtcgcgc
     3961 ccgacggttc aggccgcggc gcctccctgc tgcgccatct gggcgatgat ggcgtcggcg
     4021 aattcgtagg tgctggcggt gccgccgatg tcgcgcgtgc ggacgcggcc ctcggtgaag
     4081 accttgagca tggactgttc gatgcgatcc gccgggccgc gttcgccgag gtggcggagc
     4141 atgaggatgc cggattggag gagcgccgtc gggttggcga tgccctgccc ggcgatgtcg
     4201 ggggccgagc cgtggacggc ctcgaagacg gcgccctgct cgccgatgtt ggcgccgggg
     4261 acgaggccga ggccgccgac cagccccgcg cacaagtccg agacgatgtc gccgtagagg
     4321 ttttccagca gcatgatgtc gaactgctcg gggcgcatga cgagctgcat gcaggcgttg
     4381 tccacgatct tgtcgtcggc ctcgatctcg gggtagtcct tcgagacgtt gcggaagcat
     4441 tcgaggaaga ggccgtccga gagcttcatg atgttcgcct tgtgcaccgc cgtcaccttc
     4501 ttgcgcccct cgcggcgcgc gtactcgaag gcgtagcgcg cgatgcgggt cgaagccttc
     4561 tcggtgatga tcttgatgct ctctaccacg cccggcacga cgacgtgttc gagtccggcg
     4621 tacagactct ccgtgttctc gcgcaccacg accagatcga gttccgggta gcggcacggc
     4681 acgttcggca gcgcgcgcac gggccgcagg ttggcgtaca gatcgagctt cttgcgcagc
     4741 ccgacgttga ccgacgtgaa gcccttgccc acgggcgtcg tgatcggccc cttcaaagcg
     4801 accttgttgc gcccgatgga ttcgaggagg tcttcgggca aggtgttgcc gaacttttcg
     4861 agcgcctgcg cgcccgcgta gtgcgtctcc cactgcacct ctgcgcccga cgcctcgatg
     4921 atccgcacca ccgccgaaga gacttcgggg ccgatgccgt cgcccggaat gagtgtgata
     4981 gtatgtttca tggtcagtag tcggtagcca aagtagccag tagccagtag aaaaaccatc
     5041 ttcgccgggc agtcaggact caagcgtcaa cttccgctcg gtaaagctac tgactactgg
     5101 ctactgtcta ctgactactt gaatttttcc gcatcatacc agcaattcgg caaatgcgaa
     5161 acgagtggga ttcgggggcg gcggacgtgg tcgtcgtggg cggcggggtg atgggcctcg
     5221 cggcggcgcg ggcgctcgcg cgaaaaggtt cgagcgtcgt gctggtcgag cgcgggcgcg
     5281 tgggcgcgga ggcttcgtgg gctgccgccg ggatgctcgc gccgcaggca gaggccgacc
     5341 gccgcgacga gttcttcgac ctcctgtgcg cgggccgcga cctctacccc gcgttcgccg
     5401 acgcgctgcg cgcggagacc ggcatcgaca tcgaactcga ccgcacgggc acgctctatc
     5461 tggcgctgac cgaaagcgac gagcacgaag tcttgcggcg ctacgagtgg cagtcgcgcg
     5521 cggggcttcc cgtcgagcgg ctgacggcgg aagaggcgcg cgcctgcgag ccttccgtcg
     5581 cggagaacgt gcgggcggcc ctgcgattcc ctgcggactg gcaggtggag aaccgtcggc
     5641 tcgtcgccgc gctcgcggct tcagccgcga gcttcggcgt gcgactactc acaggggtcg
     5701 aggtcacttc gattctcgtc gagcgcgggc gggcgcgcgg cgtcgagact tcgcgcggga
     5761 ctctcgcggc gggcgccgtc gtcgtcgccg cgggcgcgtg gtcttcgctc atccgtttcg
     5821 aaagggtcgg gcgcggcgcg cagggagaag atcgcgaaga cgccgccgcg tgcccgcgcg
     5881 tcgagccggt gcgcgggcag atgctctgct tcgacgcgcg cccgcgcgtc gtgcgtcacg
     5941 tcgtctacag cccgcgcggc tacgtcgtgc cgcggcgcga cgggcgactg ctcgcaggct
     6001 cgacgaccga gcatgtgggc tatgacaagg ccgtgacggg tgaggggctg cgcgccatcg
     6061 cggcgggcgc gcaggagatc gcgccccgcg tcggcggcct cgcgctcgtc gattcgtggt
     6121 cgggcctgcg cccgcgcgcc gccgacgact ggcccgtgct cggcgcttcc gaagaagtcg
     6181 aacgcctcta ctacgcgacc ggccactacc gcaacggcat cctgctcgcg ccgttgacgg
     6241 gagaggtcgt ggccggactc gtcacggggg gcgaggctct catcaacggt cgcgcgcgcg
     6301 aagccttcgc gcccggacgc ttcctccgcg ccgcggtcgg ctcacgcgga gggcggtagt
     6361 gagcaagcat gtaccttttc actattcgct caagaataaa tcccaagtcc aaagcggctg
     6421 cgaagcacga agatgtcggc ggcgcctacg tgaattgcta catccaattc aaggattacg
     6481 aggccgccga aaagttggcc cggctgctca tcagagaaag cggctggata cctgaaaaga
     6541 ggactgaggc gtctgtcatt cagaagaggg agatgaaaac caaaaaggac aagcagtttt
     6601 attccgagac cctgaagtat ggctataccc tcgtcttcca cacgtggccg aaagacgccg
     6661 aagacgcgga tgtcgattat gaagagtagg cttctgatga agagttttat tctgcaccga
     6721 gcggctttca ttatcgcggc gacggcgctc gtctttaaca cgtcgccggg aatcgaaacc
     6781 gctgctcaat ccaacacgca gaccgaggca ggcgcgaacg gcgggggcgg cgcggcgacc
     6841 gcctcgacgg cgcaggcgct ctacgaagag gccgaaagtt acgtgcggcg caagttcgac
     6901 gagttcgcca agaagaacat cccttacagc cgcaacctcg aagcggtgac gtaccaggag
     6961 cagcgcgagc tggcgctcaa gcacgcgggc acaatcgcgg cgcgcaaggg cgagttgcgc
     7021 ggccccgacc tctaccacgc gggactgctc tacgcgctcg cgggcaagcc cgaacaggcg
     7081 ctcgacgccc tgcgccgctt cctcgccgaa gacgccgggg ccgcgcccgc tctcaggcag
     7141 aaggcgcgcg tcgtcttcgt ccagcacgcc gtcgagaagg acctcgccgc cgaggccgag
     7201 aaggttctgg ccgactacgc gcgcgaagag ccgcgcgcgc ccgccgaggc gcaccgcatg
     7261 aactcggcgc tcgccaccta ctaccacaag aagaaagatt acgagcgcgc cgccgcgcgc
     7321 gcgcgcgaag cctacggcat caccctcggg ctggcgcgcg gcaagacgct cggcgcacgc
     7381 cagcgcgccg acaccgtcta cggcacgggc gccttcctcg tcgatgcgct cgtcaaatcg
     7441 aagcagcgcg ccgaagccct cggcgtcata cagcagatgc gcagcctctc gctcgaactc
     7501 ccctcggcgc ggctctacgg caacgcgacc gtgctgctcc tcgacaacgg cgagccgctc
     7561 gcggcgccgc cgctcggcga cgcttcaacg gcgacggcgc cgcccgagat caaggtcaag
     7621 cactggatcg accagccgcc cgcgagcctc gcggacctgc gcgggcgcgt cgtgctcctc
     7681 gacttctggg caacgtggtg cggcccctgc cgcttcacca tccccaagct caacgcgctg
     7741 cacaaaaaat acaacgcccg cgggctgacc atcatcggcc tcacggagtt ctacggcaac
     7801 ggcgaaggga aagagctgtc gcccccggaa gagctggcct acctgcgccg cttcaagcgc
     7861 gagcgcgaga tcgcctacgg cttcggcgtc gccgacgaca aagagaacga cacgagctac
     7921 ggcgtcgcgc agatgccgac cgccgtcctc ctcgaccgcc gcgggcgcgt ccgcttcatc
     7981 accgtcggcg ccagcgacat cgaagcccgc gcgctcgccg agatggtcga gaaactgatc
     8041 gacgaaacgc cgtgaaccgt caagagccgt gaaccgtgac gagtcggaac agcgttcctc
     8101 tcatcacggc tcgcggctca cttcactcgt cacggcccct cgcttaccac gcatcacggt
     8161 tcgcgtcttt cactcatcac ggttcacggc ttacggctta cggctcttat gcgttccttc
     8221 atctatcgcg cacacacatg gctggggctg gcggtggcgt tgccggtgct ggcgtgggct
     8281 tcgagcgggc tgctctacgc gtggcccggc gcggtcgagg gcggcaaggt cgaggtgatc
     8341 gacaccgcgc gcgtccgcct gagcgcgccc gaagccgtcg cccgcgcgaa cgagttcgcc
     8401 ggtcgccgac tccccaccac cgcgctcacg ctcctcatgc gcgacaatcg cccctactac
     8461 caatccgtcg gcggcatggg cgccgactcg ctcctcatcg acgccgagac cggcgccgtc
     8521 ctcaaaacgc ccccgcccaa cctgaagacc cgctacttcc gccaggccca cttctacttc
     8581 ttcgccggcg cgtggcaggt gccgttgctc atcctcctct ccgccctcgc ctgcctctcc
     8641 gccgcctccg gcgtctacct gaacctgacg atgtggcgcg ggcggaagaa acggtagaca
     8701 gggaacaggt gcgccggcct ttcccagctt cgacttttcg tctttcattc tcctcgggct
     8761 tttacccttt atttacattt ccgctttcgc gctttggcgc cgggttggtg taagcttt
//
LOCUS       DBNGOH010001033         3974 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4007124, whole genome shotgun sequence.
ACCESSION   DBNGOH010001033 DBNGOH010000000
VERSION     DBNGOH010001033.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3974)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3974)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3974
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4007124"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(217..831)
                     /locus_tag="AC159P_20080"
     CDS             complement(217..831)
                     /locus_tag="AC159P_20080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697797.1"
                     /translation="MKALLTVLLLASVGVAADAQSAPPPDPPDLVVVKHSWSKERLNW
                     EGDPFSGPNENFDQLRVRMRNEKRIMDAKSGGNQVELNKVEREARADSANIERIRAQT
                     PARYGFLYKTSFRNAGGKTIKQIDWDYLFIDAATNQIVGRIELGADEKVEPGKKKDFS
                     FFLPTPPAQTISVHALNSKERAGLVEQVVLVRVVYADGTIWQRP"
     gene            complement(1127..>3974)
                     /locus_tag="AC159P_20085"
     CDS             complement(1127..>3974)
                     /locus_tag="AC159P_20085"
                     /inference="COORDINATES: protein motif:HMM:NF051698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HIR3697798.1"
                     /translation="ALSAPGVSQVVTGQSTFAGGNTTFSANGGRVRSNNFMIDGQDNN
                     DFGVAGATVGLNNPDLIQEVRLVTNQFTAEFGRNGSSVFNAITKSGTNEYHGSGFWFH
                     NDNALNACSNLEKNNGFCRPSGSTLATGQSHKRPFRVENQLGMTIGGPLHLPSFGEGG
                     PTYISGRDRTFFFFSLQRWWDRQLGSGFTINGVPNDAGRAALAAGAGTRPHVAALLSH
                     LPTANGPCTGTAAACAARTFVVNGQTFTVPIGALTGSTSFALDNWQQSIRVDHRLTEK
                     HSLFGRYIYNDNNQVGADQATPPGLSTDSLTRSQAVNLSFNSVLSSNVVNELRGAYQR
                     QGQLFTAADPRSEEIPSIEISALGLTGFNAASNRTAIGLAVNLPQFAFRQTYQLQDNL
                     SITHGNHNLKFGVDIRRNQLKQFFLPTIRGRLVYSALQRFIDDFADSATINKPLAGGQ
                     EIFYYDWHDFFGYGQTEWKVRPNFTLTLGLRYESPGQPIGDLVEVNDRIVAAAGNDQR
                     FGFTPVPERDNNNFQPRFGFNWNPRSDGGGPLGWLTGGDRLVIRGGYAVTHDYAFTNI
                     ASNIASAFPFVAAAELATVPRADGPGVPNAFATLPTIQGATGLNPNRLTRTIVGGDFR
                     SPYYQQFSLEAQRELTNDLVLRVGYVGTKGTALFQTLDGNPRRLGTATCTEANADTCR
                     VNQIQGVIRLRANAAASIYHSMQVSLEKRLSRGFSAGAHYTWSTFIDTASEIFNPSGA
                     EVAVSQDSFDRSTERARSSFDRPHRFTGNFVYELPWFREQNGFTGHVLGGWQLNSFFT
                     FQSGSPFTVLQGTDPAGALSGISGLVGNAIRPNINTTLPLGQMSVEEIRIAAGAAGAA
                     SFFAPVTAAQRVGDAGRNILRTDGINRLDFGILKNTRISESQRLQLRADFFNFTNTRD
                     FGVPEGRINNANFLNQWGTDGGKRRIIVGLRYVF"
BASE COUNT          592 a         1179 c         1505 g          698 t
ORIGIN      
        1 gcgcgccgtg cggcgctggc gggcttgaga cctgcgacgg tcagcccggc gcgcgtcgtg
       61 tggcgcgcgc cgggctgaaa tcagcgatga acttgaaatc gcaaagaacg atgcgcggcg
      121 gggaacaacg aaggcgcccc ggcgcggcag tcaagctaac accgcacggg ggcgccgtca
      181 agtagcgttg tcgcggcggc ggggcgcggc tgtggctcag gggcgctgcc agatcgtgcc
      241 gtcggcgtag acgacgcgga cgaggacgac ctgttcgacg aggcccgcgc gctctttgct
      301 gttgagcgcg tggacgctga tggtctgcgc aggcggggtc gggaggaaga acgagaagtc
      361 tttcttcttc cccggctcga ccttctcgtc ggcgccgagt tcgatgcgcc cgacgatctg
      421 gttggtcgcc gcgtcgatga agaggtagtc ccagtcgatc tgcttgatcg tcttgccgcc
      481 ggcgttcctg aaggaggtct tgtagaggaa gccgtagcgg gccggggtct gcgccctgat
      541 gcgctcgatg ttggccgagt cggcgcgcgc ctcgcgctcg accttgttca gctccacctg
      601 gttaccgccg ctcttcgcgt ccatgatgcg cttctcgttc ctcatccgca cgcgcaactg
      661 gtcgaagttc tcgttcggcc cgctgaaggg gtcgccctcc cagttgaggc gctccttgct
      721 ccagctgtgc ttgacgacga cgaggtcggg cgggtctggc ggtggagcgg actgcgcgtc
      781 cgccgcgacg cccacggagg cgagtagaag aaccgtcaac agagctttca tcgcccactc
      841 tcctttcgac ttcaggaaac tgtcggtgcc gccgggcggc aacccgcgct catcgaagtt
      901 gcgagcaaaa gtgctgtcgg gagggttgcg gcgtccgaag gtttcggcct tcggacgccg
      961 cgcgcgcgac tacgatcgtc atatcaggga cgaatgcgcc ggattatact tgttcgatga
     1021 cgacaggaaa accttcttgc agtaggaaga aagggtaggg gacgaaatct ctttcgtccc
     1081 ctacccttgc ccgtagtgcc gaccgcgcgc tggcggcggg cgcggattag aaaacgtagc
     1141 ggaggccgac gatgatgcgg cgtttgccgc cgtccgtgcc ccactggttc aggaagttcg
     1201 cgttgttgat gcgcccttcg gggacgccga agtcacgcgt gttggtgaag ttgaagaagt
     1261 cggcgcggag ctggagccgc tggctctcgc tgatgcgcgt gttcttcagg atgccgaagt
     1321 cgaggcggtt gatgccgtcc gtgcgcagga tgttgcggcc cgcgtcgccg acgcgctggg
     1381 ccgccgtcac cggcgcgaag aagctggccg cgcccgccgc acccgccgcg atgcgaatct
     1441 cctcgaccga catctggccg agcgggagcg tagtgttgat gttcgggcgg atggcgttgc
     1501 cgacgaggcc ggagatgccc gagagcgcgc ccgccgggtc ggtgccttgc agcacggtga
     1561 acggcgagcc gctctggaag gtgaagaacg agttcaactg ccagccgccg aggacgtggc
     1621 cggtgaaccc gttctgctcg cggaaccacg gcagctcgta aacgaagttg ccggtgaagc
     1681 ggtgcgggcg gtcgaacgag gagcgcgctc gctcggtgct ccggtcgaag gagtcctgcg
     1741 agaccgccac ctcggcgccc gaggggttga agatctcgga cgccgtgtcg atgaacgtgc
     1801 tccaggtgta gtgggcgccc gcgctgaagc cgcgcgagag gcgcttctcg aggctcacct
     1861 gcatcgagtg gtagatcgag gcggcggcgt tggcgcgcag gcggatgacg ccctgaatct
     1921 ggttgacgcg gcaggtgtcg gcgttcgcct cggtgcaggt cgccgtgccg aggcggcgcg
     1981 gattgccgtc gagcgtctgg aagagcgcgg tgcccttcgt gccgacgtag ccgacgcgca
     2041 gtaccaggtc gttcgtcagc tcgcgctgcg cttcgaggct gaactgctgg tagtagggcg
     2101 agcggaagtc cccgccgacg atggtgcgcg tcaggcggtt cgggttcagc cccgtggcgc
     2161 cctgaatcgt gggcagggtg gcgaaggcgt tcggcacgcc cggcccgtcg gcgcgcggga
     2221 cggtcgccag ttccgccgcc gcgacgaatg ggaaggcgct ggcgatgttc gacgcgatgt
     2281 tggtgaaggc gtagtcgtgc gtcacggcgt agccgccgcg gatgacgagc ctgtcaccgc
     2341 ccgtgagcca gccgaggggg ccgccgccgt cgctgcgcgg gttccagttg aagccgaagc
     2401 gcggctggaa gttgttgttg tcgcgctcgg ggacgggcgt gaagccgaag cgctggtcgt
     2461 tgcccgccgc cgcgacgatc cggtcgttga cctcgacgag gtcgccgatg ggctggcccg
     2521 gactctcgta ccggaggccg agcgtcagcg tgaagttcgg cctcaccttc cactcggtct
     2581 ggccatagcc gaagaagtcg tgccagtcgt agtagaagat ttcctgcccg ccggcgagcg
     2641 gcttgttgat ggtcgccgag tcggcgaagt cgtcgatgaa gcgttgcagc gcgctgtaga
     2701 ccaggcgccc gcggatggtc ggcaggaaga actgcttgag ctggttgcgg cggatgtcaa
     2761 cgccgaactt caggttgtgg ttgccgtgcg tgatcgagag gttgtcctga agctggtagg
     2821 tctgccggaa ggcgaactgc ggcaggttga cggcgaggcc gatggccgtg cggttgctcg
     2881 ccgcgttgaa gccggtcagg ccgagcgcgc tgatctcgat ggacgggatc tcttccgagc
     2941 gcgggtcggc ggcggtgaac agttgcccct gccgctggta ggcgccgcgc agctcgttca
     3001 ccacattcga cgagagcacg ctgttgaagg agaggttgac tgcctggctg cgcgtcagcg
     3061 agtcggtgct gaggcccggc ggggtcgcct ggtcggcgcc gacctgattg ttgtcgttgt
     3121 agatgtagcg accgaagagc gagtgcttct ccgtgagccg gtggtcaacg cggatcgact
     3181 gctgccagtt gtcgagggcg aaggaggtcg agccggtcag cgcgccgatg gggacggtga
     3241 aggtctgccc gttgacgacg aacgtgcggg cggcgcaggc ggcagccgtg cccgtgcacg
     3301 ggccgttcgc cgtcggcagg tggctgagca gcgcggcgac gtgggggcgg gtgcccgcgc
     3361 ccgccgcgag ggccgcgcgc ccggcgtcgt tcgggacgcc gttgatggtg aagccggaac
     3421 cgagctgccg gtcccaccag cgctggagcg agaagaagaa gaaggtgcgg tcgcggcccg
     3481 agatgtaggt cgggccgccc tcgccgaagg agggcaggtg gagcgggccg ccgatggtca
     3541 tgccgagctg gttctcgacg cggaacgggc gcttatggct ctgcccggtg gcgagggtcg
     3601 agcccgacgg gcggcagaag ccgttgttct tttcgaggtt gctgcacgcg ttcagcgcgt
     3661 tgtcgttgtg gaaccagaag cccgagccgt ggtactcgtt cgtgcccgac ttggtgatcg
     3721 cgttgaagac cgacgagccg ttgcgcccga actcggcggt gaactggttg gtgacgagcc
     3781 gcacctcctg gatgaggtcg gggttgttga ggccgacggt ggcgcccgcg acgccgaagt
     3841 cgttgttgtc ctggccgtcg atcatgaagt tgttcgagcg gacgcgcccg ccgttggccg
     3901 agaaggtggt gttgccgccc gcgaaggtgg actgcccggt cacgacctgg ctgacgcccg
     3961 gcgccgagag cgcg
//
LOCUS       DBNGOH010001034         1748 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4739758, whole genome shotgun sequence.
ACCESSION   DBNGOH010001034 DBNGOH010000000
VERSION     DBNGOH010001034.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1748)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1748)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1748
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4739758"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..644
                     /locus_tag="AC159P_20090"
     CDS             <1..644
                     /locus_tag="AC159P_20090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697799.1"
                     /translation="ESLTPEERLAFYPCIAHWIMSFADLNKHFLRVEGAADVHQQKVN
                     LYTREDDDHWRLYVEDFRKLGCERFRDGTDWLSFLRGEETRANRMLSYRIAHLVVGAS
                     SVQRLAIVESMEEAANVFFPLTLRLAERIQERTGTELRYLGHFHFDLEAGHTGAGDYE
                     TLARVELDDLARHKTVEMVNGIFRLYEDWADETLRFAATQLADTLPAERAMCA"
     gene            complement(825..1376)
                     /locus_tag="AC159P_20095"
     CDS             complement(825..1376)
                     /locus_tag="AC159P_20095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697800.1"
                     /translation="MRRVFAVTLLLGLCLHGSGAAAQRTNAERRAAELAAFFSKRKHE
                     VREKKGVRVEKFKEVKSEPAVRGDAAAYAGEYESDTGCGLTLRVVAGGAVEAAGCEPG
                     PDGNRRFTLRGARLEGALLTGTKVYDDGTNEKFEGLFINRTDRDSSTDAGTTTFGLGV
                     IFDPPKTAHNGSYTLSRLFYRKK"
     gene            complement(1488..>1748)
                     /locus_tag="AC159P_20100"
     CDS             complement(1488..>1748)
                     /locus_tag="AC159P_20100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697801.1"
                     /translation="SGELAARVVSDWLPGARRDFDALARAYAAHYAARFDWRLRLCGL
                     LRRAAFAPRALTEAAALALNASSRLRHRLARATRRAGARAPV"
BASE COUNT          288 a          608 c          557 g          295 t
ORIGIN      
        1 acgagagcct cacgcccgag gagcggctgg ccttctaccc atgcatcgcg cactggatca
       61 tgagcttcgc cgacctcaac aaacacttcc tccgcgtcga aggcgcggcg gacgttcacc
      121 agcagaaggt caacctctac acgcgcgagg acgacgacca ctggcgcctc tacgtcgagg
      181 acttccgcaa gctcggctgc gagcggttcc gcgacggcac cgactggctc tccttcctcc
      241 ggggcgagga gacgcgcgcc aaccgcatgc tctcgtaccg catcgcgcac ctcgtcgtgg
      301 gcgcgtcgag cgtccagcgg ctcgccattg tcgagtcgat ggaggaggcc gccaacgtct
      361 tcttcccgct cacgctcagg ctcgccgaac ggattcagga gcggaccggc acggagctgc
      421 gctacctcgg ccacttccac ttcgacctcg aagccgggca caccggcgcg ggcgattacg
      481 aaaccctcgc gcgcgtcgag ctggacgacc tcgcgcgcca caaaaccgtc gagatggtca
      541 acgggatttt ccgtctctac gaagactggg ccgacgaaac cctccgcttc gccgccaccc
      601 agctcgccga cacgctcccc gccgaacgcg ccatgtgcgc gtgagagaaa gtaagcagca
      661 ggcagcgggc ggcaggcagt aaaagcaggt agggcgaaga ggcttgagag gtaaaatgct
      721 atgcctcttc gtttgatagg cggtaaagtg aagcggcttg agagattcag tctcaagccg
      781 cttccttctt ctgttttgac tgcctgctgc ccgctgccta ctgcctattt cttcctgtag
      841 aacagacggc tgagcgtgta gctgccgttg tgtgcggtct tgggcgggtc gaagatgacg
      901 ccgaggccga aggtcgtcgt gcccgcgtcc gtggagctgt cgcgatccgt ccggttgatg
      961 aagagaccct cgaacttctc gttcgtcccg tcgtcataga ctttggtgcc ggtcagcaat
     1021 gcgccttcga gccgggcgcc ccgcagcgtg aaccggcggt tcccatcggg gccgggttcg
     1081 catcctgcgg cctcgaccgc gccgcccgcc acgacacgca gcgtcaaccc gcagccggtg
     1141 tccgactcgt actcgcccgc gtacgccgcg gcgtcgcccc gcacggctgg ctcgctcttc
     1201 acctctttga acttctccac gcggacgccc ttcttctctc tcacctcgtg cttgcgcttg
     1261 ctgaagaagg cagccaactc cgcggcacgc cgctcggcgt tcgtgcgctg tgccgccgcg
     1321 ccggaaccgt ggaggcagag gccgagaagt aacgttacgg caaaaactct tcgcatgtct
     1381 ttctcccctt ccttgttgtg ctcgtgccga atgcagactc tcaaactgta aacaccgccg
     1441 cgcacgcctt cgtgacaagc atctcgcgcg cgcgcccccg ccttaaccta cacaggcgca
     1501 cgcgcaccgg cgcgtcgcgt cgcgcgcgcg agtctgtggc ggaggcgcga gctggcgttg
     1561 agggcgaggg cggcggcttc cgtgagcgcg cgcggggcga aggcggcgcg gcgcagcagg
     1621 ccgcagaggc gcaggcgcca gtcgaagcgc gcggcgtagt gggcggcata ggcgcgggcg
     1681 agcgcgtcga agtcgcggcg cgcgcccggc agccagtcgg agacgacccg cgcggccagc
     1741 tctccgct
//
LOCUS       DBNGOH010001035         3585 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3639277, whole genome shotgun sequence.
ACCESSION   DBNGOH010001035 DBNGOH010000000
VERSION     DBNGOH010001035.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3585)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3585)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3585
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3639277"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..765
                     /locus_tag="AC159P_20105"
     CDS             <1..765
                     /locus_tag="AC159P_20105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697802.1"
                     /translation="AAPHARAVDEEVVTDLRVRDLELDEQWSFAGSKKAAFSESAERG
                     EAWWHKAMARESRLIVEQFVSPRTPEAAELLVNRSFDRLASGCLPRVSSDGYDAYTQP
                     LSEQVRALTVYPLQWALMKGKRGRPPKPKQIPDPALLYGQVVKEREGRRVVKVERRLV
                     LGPPRAELAFISTSLLERQNGTARSRNRYLVRKTYAFAKTVPYMDDQCEVDKTFYNFC
                     RKHRGLKGETPAMRQGITDHVWSVAEVLGYRSAAPG"
     gene            complement(921..1361)
                     /locus_tag="AC159P_20110"
     CDS             complement(921..1361)
                     /locus_tag="AC159P_20110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697803.1"
                     /translation="MRAFVLTLAACAACAVLVACGAPADNTTTTTNTKTTTTNASGNT
                     TTTTTTTTNTANTAATPASTTTTTAAVTGVAECDEYISKYEACVKDKVPASMRAQLQA
                     TLDASRNAWKTAASTPQGKATLAKTCTDALAAAKQAMGSYGCNF"
     gene            complement(1634..2134)
                     /locus_tag="AC159P_20115"
     CDS             complement(1634..2134)
                     /locus_tag="AC159P_20115"
                     /inference="COORDINATES: protein motif:HMM:NF022112.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HIR3697804.1"
                     /translation="MTRSLQSFAALALLLVCTAGEAQTATPARLREVKIYLVNSEAEY
                     DAKNPFGLQAVARKVDARRPLRAALAALVAGPTAAEEERGLGSSTYGIRFISVKLKRG
                     VAHAHFALPNDRGFPGDLAPAIFKQAVTKTATQFPNVRRTVICLDGDLNFDDESGAPP
                     KECPKV"
     gene            complement(2213..3223)
                     /locus_tag="AC159P_20120"
     CDS             complement(2213..3223)
                     /locus_tag="AC159P_20120"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HIR3697805.1"
                     /translation="MFTEEVLLAVSVTDEKGRFDPSLEKEELLVLEDGVPQEIRSARR
                     VPASVLLLVGTGGEMNPSMKTNTTREFVARLVARLAAGDRIAAMQYGGDAEIVQDWTT
                     DGAAALEAIRRKLSSKRGARLAAAMRAAAARFEEMPQGNRHLVLVTDGVDDAAEGKAG
                     LEEAVKTLLAAGVAVHVISYSRMGRRALRRVAPPVQVTGEKTRRTAADIARELMNPAK
                     PPVDVPDIFVTIDLDFPMRRKRAAYTRAMREGEQWLGPFAEETGGTTLLPLSEAEMLA
                     GSDALARAIDSRYVVTYRPKRPLASAASGEYRRVAVVARRVGLRASARRGYVVTPTPA
                     RR"
     gene            3216..3401
                     /locus_tag="AC159P_20125"
     CDS             3216..3401
                     /locus_tag="AC159P_20125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697806.1"
                     /translation="MNTRTVSCCVPVCGDDDAGGAVVVGAGEACGAVCAGQTSAAEQT
                     KSTGHDLDETRLDFMPV"
     gene            complement(3449..3574)
                     /locus_tag="AC159P_20130"
     CDS             complement(3449..3574)
                     /locus_tag="AC159P_20130"
                     /inference="COORDINATES: protein motif:HMM:NF039182.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5654 family protein"
                     /protein_id="HIR3697807.1"
                     /translation="MAALYAYAVIATVIAVVVVMLLGRAAARVGGAAAISREAEG"
BASE COUNT          513 a         1249 c         1231 g          592 t
ORIGIN      
        1 gccgccccgc acgcccgcgc cgtcgatgag gaggtagtca ccgacctcag agtccgcgac
       61 ctggagttgg acgagcagtg gtcgttcgcc gggtcgaaga aggccgcctt ctcggagagc
      121 gccgagcggg gcgaggcgtg gtggcataaa gcgatggcgc gggagagccg gctcatagtc
      181 gagcagttcg tctcaccgcg caccccggaa gccgccgaac tgctcgtaaa tcggagcttt
      241 gaccggctcg cctcggggtg cctgccgcgg gtcagcagcg acggttacga cgcttatacg
      301 cagccccttt ccgagcaggt cagggctctc accgtctacc cgttgcaatg ggcgctgatg
      361 aaaggaaagc gcgggcgacc gcccaagccg aagcagattc ctgacccggc gctcctctac
      421 gggcaggtgg tcaaggagcg cgaaggccgg agggtggtga aggtcgagag gcggctggtg
      481 ctggggccac ccagggccga gttggctttt atctcgacct cattgcttga gcggcagaac
      541 gggacggcgc ggtctcggaa ccgctacctc gtgcgcaaga cctacgcctt cgctaagaca
      601 gtgccctaca tggacgatca atgtgaggtg gataagacct tctacaactt ctgccgtaaa
      661 catcgcgggc tcaaaggcga gacgcccgcg atgcggcaag ggatcaccga tcacgtctgg
      721 tcggtggccg aagtgttggg ctaccgaagt gccgcaccgg gatgaatccc caacgtaacg
      781 gggccactac cccgcgccgg ggcggcggga ccgcgaggcg gtgtggggct tcgggggaaa
      841 atagaggcgc gcgggccgcc ttcggactcg gagtgccgga gacggcccgc gcgcgcgaga
      901 ttttgatgag gggctgcggg ttaaaaattg cagccgtatg atcccatggc ctgcttggcg
      961 gcggcgagtg cgtccgtgca ggtcttcgcc agcgtggcct tcccctgcgg ggtcgaagcg
     1021 gcggtcttcc acgcgttgcg gctcgcgtcc agcgtggcct gcaactgcgc gcgcatcgaa
     1081 gccggcacct tgtccttcac gcacgcctcg tacttgctga tgtactcgtc gcactcggcc
     1141 acgccggtca cggcggcggt cgtcgtggtg gtgctcgcgg gcgtcgccgc ggtgttcgcg
     1201 gtgttcgtgg tggtcgtggt ggtcgtggtg gtattgcctg acgcgttggt ggtcgtcgtc
     1261 ttggtgttgg tcgtcgtggt ggtgttgtcc gcgggcgcgc cgcacgccac gagcacggcg
     1321 caggccgcgc aggcggccag cgtcagcaca aaagctctca tagtctggtt ctcctcgacg
     1381 cgtccggcca cgggccgtcg cgcgtgtgcg aatcggttcg ggcgccggca gcgaccgcgc
     1441 gcggaggcgc gacgcgtggc ggcggcttgt cagcgaggcg tcggaaggcg tgttgcagac
     1501 cgccctgtgg ttcgttccgg atagtgcttg aagtgccgcg gcatcttaac accgttttcg
     1561 gttccgcaca caaggcggcg cgcggcctct tcgacgcgtc gggaaagtgt cgggcggggc
     1621 ggcccggcgg gcttcaaact ttggggcact ccttgggcgg ggcgccgctc tcgtcgtcga
     1681 aattcaaatc gccgtcgagg cagatgactg tgcggcggac gttcgggaac tgcgtcgccg
     1741 tcttcgtgac ggcctgcttg aagatggccg gggcgaggtc gccggggaag ccgcggtcgt
     1801 tcggcagcgc gaagtgcgcg tgggcgacgc cgcgcttcag cttcaccgag atgaacctga
     1861 tgccgtaggt cgaactcccg aggccgcgct cctcttccgc cgcggtcggc cccgcgacga
     1921 gcgccgccag cgccgcgcgc aagggtctgc gcgcgtccac cttgcgcgcc accgcttgca
     1981 gcccgaacgg gttcttcgcg tcgtactcgg cctcgctgtt gaccagatag attttgactt
     2041 ccctcaaacg cgcgggcgtc gcggtctgcg cctccccggc tgtgcagacg aggagcagcg
     2101 cgagcgccgc gaaggattgc agacttctcg tcaactttcg gttcctcatc cggacccttt
     2161 ccgccggggc ttcctgtgcg cccggccaga tgctttgcct cttccactcc gctcaacgac
     2221 gagccggcgt cggcgtcacg acgtagccgc gccgcgccga cgcgcgcagc ccgacgcgac
     2281 gcgcgacgac cgcgacccgc cggtactctc cgctcgcggc ggaagcgagc gggcgcttcg
     2341 gcctgtacgt gacgacgtac cgcgagtcga tggcgcgcgc gagcgcgtcc gatcccgcca
     2401 acatctccgc ctccgacagc ggcagcagcg tcgtgccgcc cgtctcttcc gcgaacggcc
     2461 ccaaccactg ctcgccctcg cgcatcgcac gcgtgtacgc ggcgcgcttg cgacgcatcg
     2521 ggaagtcgag gtcgatggtg acgaagatgt ccggcacgtc cacggggggc ttggccggat
     2581 tcatcaactc gcgcgcgata tccgccgccg tccgcctcgt cttctcccct gtgacctgca
     2641 ccggcggggc gacgcggcgc agcgcgcgcc ggcccatccg cgagtagctg atgacatgca
     2701 ccgcgacgcc cgccgcgagc agcgtcttca cggcctcttc gagaccggcc ttgccctcgg
     2761 cggcatcgtc cacgccgtcc gtgacgagca cgagatggcg gttgccctgc ggcatctctt
     2821 cgaagcgggc ggcggcagcc cgcatcgccg ccgcgagccg cgcgcccctc ttcgacgaca
     2881 gcttacgccg aatcgcttcg agcgcggcgg cgccgtccgt cgtccagtcc tgaacaatct
     2941 ccgcatcgcc gccgtactgc atcgccgcga ttcggtcgcc cgccgcgagc cgcgcgacga
     3001 gccgcgcgac gaactcgcgc gtcgtgttgg tcttcatcga ggggttcatc tcgccccccg
     3061 tgccgacgag cagcaggacg ctggccggca cgcgccgcgc actcctgatc tcctgcggca
     3121 cgccgtcttc gagcaccagc agctcttcct tttcgagcga cgggtcgaag cgccccttct
     3181 cgtccgtcac gctgacggcg agcagcacct cctccgtgaa cacgcggacg gtctcctgct
     3241 gcgtgccggt ctgcggagac gacgacgcgg gcggcgcggt cgtcgtcggg gcgggtgaag
     3301 cgtgcggcgc cgtctgcgcc ggacagacca gcgccgccga acagacgaag agcaccggcc
     3361 acgacctcga cgaaacacgt ctggacttca tgccggtctg atgcgaccgt tcagcccgcg
     3421 cgatgtcttc ggcccgcggc cctcgtcctc agccctcggc ctcgcggctg atggccgccg
     3481 cgccgccgac ccgcgccgcc gcccgcccca gcagcatcac caccaccacc gcgatcaccg
     3541 tcgcgatcac agcgtatgcg tacagcgccg ccagcccctc gtccg
//
LOCUS       DBNGOH010001036         1589 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2724496, whole genome shotgun sequence.
ACCESSION   DBNGOH010001036 DBNGOH010000000
VERSION     DBNGOH010001036.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1589)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1589)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1589
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2724496"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..316
                     /locus_tag="AC159P_20135"
     CDS             <1..316
                     /locus_tag="AC159P_20135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697808.1"
                     /translation="LQVFVYSDEDDGPAPHSPDATDIGVGTLKLRRERDGESDGRVYL
                     IVVKATDASGNTAVSVTTVTVPLSSSAGDQASVNAQAAAAAAFAAANNGAAPAGYFPV
                     GL"
     gene            complement(515..1450)
                     /gene="speB"
                     /locus_tag="AC159P_20140"
     CDS             complement(515..1450)
                     /gene="speB"
                     /locus_tag="AC159P_20140"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HIR3697809.1"
                     /translation="MSESAHLPMNFGGIEDEALSSFEQARVVVWPISYEGTVSYGGGT
                     GRGAEAIIDASRNMELYDEETDAEVYQLGIHTVETSPPLEPPERMMEALLARARELVS
                     TGKFVCMIGGEHSVSGPVIAAHAERYENLSVLQIDAHADLRDTYDGTKHSHASIMARV
                     VKDLKVPAVQVGIRSISSEEARVIDELPTRIFWAKDIVGRTDWWEEVVSGLTENVYLT
                     IDIDGLDPSLVAQTGTPEPGGLGWYETLGLVRTLARSRRVVGMDLNEYAYVEGHSASA
                     FLCAKLIYKSLSYIFESETERVRGSSRARMAGGRQ"
BASE COUNT          263 a          595 c          465 g          266 t
ORIGIN      
        1 cttgcaagtg ttcgtctact ctgacgagga cgacgggcct gcgccccact cgcccgacgc
       61 gacggatatc ggcgtcggca cgctcaagct ccgcagggag cgggacggcg agtcggacgg
      121 tcgcgtctac ctcatcgtgg tcaaggcgac ggacgcctcg ggcaacaccg ccgtgagcgt
      181 caccaccgtc accgtgcccc tgagcagcag cgccggggat caggcgtcgg tcaacgcgca
      241 ggcggctgcg gcggctgcct tcgccgcggc caacaacggg gcggcgcccg ccggctactt
      301 cccggtcggc ctgtagcccc tctgaccgag gcggcctgac tctcgaaggt cgcgccgcct
      361 cgcttgaacc gaccggggcc gcggtacagg gaatgtaccg cggccccggt tcgttctgcc
      421 cgcgccggcg tcaaagggcg aagaggcttg agatgtagag tctcaagcct cttccttcag
      481 ccttctactt ctgtttttac tgcccgctcg ccgcctactg cctgccgccc gccatcctgg
      541 cgcgcgacga gccgcgcacg cgctcggtct ccgactcgaa gatgtaggag agcgacttgt
      601 agatgagctt ggcgcagagg aaggccgagg cgctgtgccc ctcgacgtag gcgtactcat
      661 tcaagtccat gccgacgacg cggcgcgagc gcgcgagcgt gcggacgagg cccagagtct
      721 cgtaccaccc gaggccgccc ggctccggcg tccccgtctg cgcgacgagc gacgggtcga
      781 ggccgtcgat gtctatcgtc aggtagacgt tctccgtcaa tcccgacaca acctcctccc
      841 accagtccgt gcgccccacg atgtccttcg cccagaagat gcgcgtcggc agctcgtcga
      901 tcacgcgcgc ctcttcggac gagatcgagc ggatgccgac ctgcaccgcc ggcaccttca
      961 agtccttcac cacccgcgcc atgatcgacg cgtgcgagtg cttcgtcccg tcgtacgtgt
     1021 cgcgcaaatc ggcgtgcgcg tcgatctgca acaccgacaa attctcgtac ctctcggcgt
     1081 gcgccgcgat caccggcccg ctcaccgaat gctcgccgcc gatcatgcac acgaacttgc
     1141 cggtcgagac caactcgcgc gccctcgcca gcagcgcctc catcatccgc tcgggcggct
     1201 ccagcggcgg cgaagtttcg accgtgtgga tgccgagttg atacacctcc gcgtccgtct
     1261 cctcgtcgta caactccatg ttccgcgacg cgtcgatgat cgcctccgcc ccgcgccccg
     1321 tccccccgcc gtacgacacc gttccctcgt acgagatcgg ccacaccacg acgcgcgcct
     1381 gctcgaaact cgacagcgcc tcgtcctcga tgcccccgaa gttcatcggc aaatgtgctg
     1441 attcgctcat gcgccgagta taacgcacgc ccattcgaac cgggcaagcc ttgaactgcg
     1501 gtcacgccat cggcgataat cgccgcatga gcgaagaccc cacacaacac ttgcccaatg
     1561 acaacttcaa gctcatcctc gcgcgcttg
//
LOCUS       DBNGOH010001037         4170 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2357131, whole genome shotgun sequence.
ACCESSION   DBNGOH010001037 DBNGOH010000000
VERSION     DBNGOH010001037.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4170)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4170)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4170
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2357131"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            306..1517
                     /locus_tag="AC159P_20145"
     CDS             306..1517
                     /locus_tag="AC159P_20145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3697810.1"
                     /translation="MKNVLRLLATAAVAAAFALPAYAQDAATQDAAAQENEARAALYQ
                     KFLELRVKKDAQGNLDPAAQKQAYETGKEYLSKYSSNANEDNAAIVAYIRKWVKDYED
                     AVRDFTFRQAIDKGDFKGAIPPGRELLAINPENVRVNLLLARTGYVAVSKGDKAATSD
                     ALGFIRRTMQLVEAGKVADNWAPFVSKEDALGWLSYMEGFLILEGQPDAAVKSLLKAV
                     QSNSAASKEPSAYDTLAFIYTKEYQRAAAEYQQKFPQGTEITPELKPEYDRLQVRLDK
                     IADRIIDAQARVVAFSTKPDQQELKNRVMTRLTAIYKSRHNESDAGLKELIAGVASKP
                     IMIPGQEPEPTPSTPTTTPSTTPTTGGSTQASQTGRPAAATTPGGANTAKPAAATATP
                     QPKPATTAPRP"
     gene            1690..2454
                     /gene="rnc"
                     /locus_tag="AC159P_20150"
     CDS             1690..2454
                     /gene="rnc"
                     /locus_tag="AC159P_20150"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HIR3697811.1"
                     /translation="MNEDTPPAQDLSPLEEALDYSFRNRALLERAITHRSWAHEHVRA
                     GEEEKARHMHNEALEFVGDSVLGLVVADTLFRTHTELTEGEMSRMKHRLVSTHTLARA
                     ARRLGLGDHIRMGRGEEKTGGRRKRALLADAFEAVLAAIYVDGGFECAAAFVRRALET
                     EFAEATPEAAAAADYKTLLQERLQAERRVTPQYQLVEQHGPPHARVFHVEVQWDEGRV
                     RGEGHTIKAAEMDAACRALEQLSEAGDEQETVSREQ"
     gene            2683..3645
                     /gene="lepB"
                     /locus_tag="AC159P_20155"
     CDS             2683..3645
                     /gene="lepB"
                     /locus_tag="AC159P_20155"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HIR3697812.1"
                     /translation="MNIEQREGRAAAAEEVRARKPEPNGAGGGGAEAEGAVTHGPPRS
                     VLREYFESLVVTAIMAIFGMTFVVQAVKVPTGSMQNTILIGDHLLVNKFIFAPGPVMP
                     FLPQREIRRGDVIVFKYPGKYEADERFRDNPEVNDKAPDNIPFKINYVKRVVGLPGER
                     IELRGTDIYADGQLVKEHKVAALNPPRDDDPRTSGDESGAPLHDERDVYLTGDNAPPR
                     EEGAHTVYWAPETLRRDVQGDEDGMVFDVPAGHYLTLGDNRENSLDGRYWGYVPRELV
                     IGRAMFVYWSFDESAPRSDLTLPFNYVADFFTNTRWGRTGTLVR"
     gene            3806..>4170
                     /locus_tag="AC159P_20160"
     CDS             3806..>4170
                     /locus_tag="AC159P_20160"
                     /inference="COORDINATES: protein motif:HMM:NF015587.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HIR3697813.1"
                     /translation="MFQSLSLGGLSWTELGKRVWAEVQEDDVLGRAAQLAYYFLLALF
                     PALLFLVSLIGYFAGEDSELRENLFNYLNTVLPGEASQLISKTVTDVTQGSGGGKLSF
                     GILATLWAASNGMGAISETL"
BASE COUNT          790 a         1465 c         1340 g          575 t
ORIGIN      
        1 cgctgctaaa acagcggctc cgggcctccc ggtttggggc cggaaattta caacgttttt
       61 cttgacaccg ccggaaaagt tggtcagtat cacggcgcga taagcgctct cgtgtcaccc
      121 gtccgaagaa ccccttaaca ccgctccccc gttcggaacg ggccgctccg gacgccccgc
      181 cgagggacgg caaccggccc gaaggggcga gcatctgaat ggtacagcga cgcccgcgac
      241 gaaggcttcg cgcccccatg gaagagccga actcccccac acatcgcacg gaggatttgc
      301 gaccgatgaa aaacgtctta cgactgctcg ccaccgccgc cgtggcagcc gccttcgcgc
      361 tccccgccta cgcgcaggac gccgccacgc aggacgccgc ggcgcaggag aacgaggcgc
      421 gggccgcgct ctaccagaag tttctcgaac tgcgcgtgaa gaaggacgcg cagggcaacc
      481 tcgacccggc ggcgcagaag caggcttacg agaccggcaa ggagtacctc tccaagtaca
      541 gttccaacgc gaacgaggat aacgcggcca tcgtcgcgta catccggaag tgggtgaagg
      601 actacgagga cgcggtgcgc gacttcacct tccggcaggc catcgacaag ggcgacttca
      661 agggggctat cccccccggt cgcgagctgc tcgccatcaa ccccgagaac gtgcgcgtca
      721 acctcctgct cgcgcgcacc ggctacgtcg ccgtgagcaa gggcgacaag gccgccacca
      781 gcgacgccct cggcttcatc cgccgcacca tgcagctcgt cgaggccggc aaggtggccg
      841 acaactgggc gcccttcgtg agcaaggaag acgcgctcgg ctggctcagc tacatggagg
      901 gcttcctcat ccttgagggc cagccggacg cggcggtcaa gtcgctcctc aaggcggtgc
      961 agtcgaacag cgcggccagc aaggagccga gcgcctacga cacgctcgcc ttcatctaca
     1021 ccaaggagta ccagcgggcc gccgccgagt accagcagaa gttcccgcag ggcacggaga
     1081 tcacgccgga actcaagccc gagtacgacc gccttcaggt gcggctcgac aagatcgccg
     1141 accgcatcat cgacgcgcag gcccgcgtcg tcgccttcag cacgaagccc gaccagcagg
     1201 aactcaagaa ccgggtgatg acgcggctga cggcgatcta caagagccgc cacaacgagt
     1261 cggacgccgg cctcaaggag ctgatcgcgg gcgtcgcgtc gaagccgatc atgatccccg
     1321 gccaggagcc ggagccgacc ccttcgacgc cgaccacgac gccttcgacg acgccgacca
     1381 ccggcggctc cacgcaggcg agccagaccg gcaggcccgc cgcggccacg acgcccggcg
     1441 gcgcgaacac ggccaagccc gccgccgcca ccgcgacgcc gcagccgaag cccgcgacga
     1501 cggccccccg cccgtgaggc cgcagccgtc ctcgccggac gcgtgaaggc gtaggccgcg
     1561 cgcccggtcg gccccgtgcc gcccgagcgc gagccggtcg agaaaaccga atcctccgtc
     1621 cgcgccgggc gtgccgttga agaatcccaa acggcacgcc cggcgttttt gttacaatcc
     1681 cgccccgaga tgaacgaaga cacgccgccc gcacaagacc tctcgccgct cgaagaggcg
     1741 ctcgactact ccttccgaaa ccgcgcgctg ctggaacgcg ccatcacgca ccgctcgtgg
     1801 gcgcacgagc acgtgcgcgc gggcgaggag gagaaggcgc gccacatgca caacgaggcg
     1861 ctcgaattcg tcggcgactc ggtgctcggc ctcgtcgtcg ccgacacgct cttccgcacg
     1921 cacacggagc tgaccgaggg cgagatgtcg cgcatgaagc accggctcgt gagcacgcac
     1981 acgctggcgc gcgccgcccg ccgcctcggc ctcggcgacc acatccgcat gggccgcggc
     2041 gaggagaaga ccggcgggcg ccgcaagcgc gcactcctcg ccgacgcctt cgaggcggtg
     2101 ctcgcggcca tctacgtgga cggcggcttc gagtgcgccg ccgccttcgt ccgccgcgcg
     2161 ctcgaaaccg agttcgcaga ggcgacgccc gaggccgccg ccgccgccga ctacaagacg
     2221 ctcttacagg aacgcctcca ggccgaacgc cgcgttaccc cgcaatacca gctcgtcgag
     2281 cagcacggcc cgccgcacgc gcgcgtcttc cacgtcgaag tccaatggga cgagggccgc
     2341 gtccgcggcg agggccatac catcaaggcc gccgagatgg acgccgcctg ccgcgcgctc
     2401 gaacaactct ccgaagcggg cgacgagcaa gagacggtga gccgcgagca gtaagcaagt
     2461 agcaggcagc aggcaggagg cagtcaaaac agaagaagcc agaggaggct tgggaggtaa
     2521 agtcccaagc ctctccgctt ctcaagcggt aaagcaagag gcttgagacg tggccgctca
     2581 agcctcttcg cgcttttgtt tgaactgcct gctgcctgcc gcccgctgcc tgctactttc
     2641 cccgcttgac cgtttcgggc ggcatcacta cgatgaatcc atatgaacat tgagcagcgg
     2701 gaagggcgcg cggcggcggc ggaagaagtg cgcgcgcgga agccggagcc gaacggcgcg
     2761 ggcggcggcg gggcggaggc cgaaggcgcc gtgacgcacg ggccgccgcg ctcggtgctg
     2821 cgcgaatact tcgagtcgct ggtggtgacg gcgatcatgg cgatattcgg catgacgttc
     2881 gtcgtgcagg ccgtcaaggt gccgaccggc tcgatgcaga acacgatcct catcggcgac
     2941 cacttgctcg tcaacaagtt catcttcgcg cccggcccag tcatgccctt cctgccgcag
     3001 cgcgagatcc gccgcggcga cgtgatcgtc ttcaagtacc ccggcaagta cgaggccgac
     3061 gagcggttcc gcgacaaccc ggaagtgaac gacaaggccc ccgacaacat ccccttcaag
     3121 atcaactacg tcaagcgcgt cgtcggcctg cccggcgagc gcatcgagct tcgcggcacg
     3181 gacatctacg ccgacgggca gttggtcaaa gagcacaagg tcgccgcgct caatccgccg
     3241 cgggacgacg acccgcgaac gagcggcgac gagagcggcg cccccctgca cgacgagcgc
     3301 gacgtgtatc tcacgggcga caacgcgccg ccgcgcgaag agggcgcgca caccgtctac
     3361 tgggcgccgg agaccttgcg gcgggacgtg cagggcgacg aggacgggat ggtcttcgac
     3421 gtgcccgccg gtcactacct cacgctcggc gacaaccgcg agaacagcct cgacggtcgc
     3481 tactggggct acgtcccgcg cgagctggtc atcggtcgcg ccatgttcgt ctactggtct
     3541 ttcgacgaga gcgccccccg tagcgatctc acgctcccct tcaactacgt ggccgacttt
     3601 ttcaccaaca cgcgctgggg ccgcacgggc acgctcgtcc gctagctgcc gctgcgcgga
     3661 aggcaaaagg caaaaggcaa aagttaaaag gaaggacaaa gcttcgcttt agtatttttg
     3721 attttttcgg atcgcgcgca gcgcgtccac ccttttgcct tttgcctttt accttttgcc
     3781 ttttgcctta tcaaggaggg gagacatgtt ccaatcgctc agtctcggcg gtttgagttg
     3841 gacggagctg gggaagcgcg tctgggcgga ggtgcaggag gacgacgtgc tgggccgcgc
     3901 ggcgcagctc gcctactact tcctgctggc gctcttcccg gcgctgctgt tcctggtctc
     3961 gctcatcggc tacttcgcgg gcgaggacag cgagctgcgc gagaacctgt tcaactacct
     4021 gaacacggtg ctgcccggcg aagcctcgca gctcatcagc aagaccgtga cggacgtgac
     4081 gcaggggagc ggcggcggca agctctcgtt cggcatcctc gccacgctgt gggccgcctc
     4141 gaacgggatg ggcgccatca gcgagacgct
//
LOCUS       DBNGOH010001038         5621 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1076391, whole genome shotgun sequence.
ACCESSION   DBNGOH010001038 DBNGOH010000000
VERSION     DBNGOH010001038.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5621)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5621)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5621
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1076391"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..443
                     /locus_tag="AC159P_20165"
     CDS             <1..443
                     /locus_tag="AC159P_20165"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR3697814.1"
                     /translation="SVVGDGRLAGELRERAERLGIAGRVDWHGVVRDPYAFYRASSIF
                     ALPSRVEGTPNALLEAMSCGMPVVVSDGAPGPLELVEDGVTGLVVPVNDTAALAAALS
                     RLARDEELRGRLGEAACARVSEYDLPRALAAWESAIGLTNQQKP"
     gene            557..2533
                     /locus_tag="AC159P_20170"
     CDS             557..2533
                     /locus_tag="AC159P_20170"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013535048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3697815.1"
                     /translation="MSTRPSSTRRHAAPPPKPTLRERVAALRYVPPFVRLVWQTHRGM
                     TVAMMVLRLVRAFVPVAVLWVGKLIVDEVVAASGAGPGAADLSRLWRLVAVEIALALL
                     GEVLARASSLVESLLGDLFSNHTSVRLMSHAATLDLYQFENPDFYDRLDRARRQTTGR
                     IGLLAQLLSMGQDALTLLSLGAALLVYSPWLLLLLAVAVLPGFLGEAHYAALEYSLLY
                     RWTPERRQLDYLRLLGASDRPAKEVQMFGLARWLVERYRALSDKFYEENKRLSVRRST
                     VAAALSAFGVLGYYGAYAVILARAVAGAISLGTLTFLAASFLRSRDLIQRLLSGASGI
                     YQQCLYLKDLFDFFETEPQISSRAGAPAVPRTLREGFRFENVGFRYPDSERWSLRGVT
                     FRLRPGERLALVGGNGAGKTTLVKLLARLYDPTEGRILLEGRDLRDYDLASVRRSVGV
                     IFQDFFRYDLRLDENIGVGEIEDVRHYLDTADEMHDASHQKPRGDREKKAGGDDGNAS
                     RDEGNGATPPAAVVAAAEKSLADTLLARLPAGYRQMLGRRFEGGVDLSGGEWQKVALA
                     RAYMRDAHVLILDEPTSALDARAEYEVFRRFAELMRGRMAVIISHRFSTVRMADRIIV
                     LEDGVIVEEGTHAALVERGGLYAELFALQAEGYR"
     gene            complement(2618..4009)
                     /locus_tag="AC159P_20175"
     CDS             complement(2618..4009)
                     /locus_tag="AC159P_20175"
                     /inference="COORDINATES: protein motif:HMM:NF025056.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HIR3697816.1"
                     /translation="MNIFICWSGDRSKALAKLMYEYLPRFIPDLPPPFFSENIVKGTR
                     WFDEVSVALDAADAGLVCVTPESLHSGWIHFETGALARAVRKNRRGKVFVYLLNVEPD
                     ELRGPFGAFQSTRSDRDDTEKLIDAIVTSMGADADAREKCERAFAHHWGAFEKGLAAI
                     RPPTIDELIPGLEQLFRRKTFNEPLDECTRQTWLDRYDGARQTLERLSRYRQVMEADD
                     SHLSDLYHELVGQLDGYAMDMSALLLREEHFEIGGDGKVSLGEGVRRPCEERRRRIRQ
                     LVVRLLAADGGPVLPESRRFARLHTFEEKKALLIHPQQQRFQLGKGKGARPREETLRR
                     CAASWWEFDRIYYYLAQEYGDAPDVARLNECLEQEFERLRAKDGGGSLIPLHYAVRAL
                     KKALTQSAALGEAREDAARLRRLVGRLRSYLAQVGQDAGGQVGDNLEDLSALLPEAAN
                     GCADARAAESPVA"
     gene            4318..5535
                     /locus_tag="AC159P_20180"
     CDS             4318..5535
                     /locus_tag="AC159P_20180"
                     /inference="COORDINATES: protein motif:HMM:NF037429.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baeRF10 domain-containing protein"
                     /protein_id="HIR3697817.1"
                     /translation="MAQSKEAVEGGTLDDLLERLAAFEPTGLPFISLYLNAQANENGR
                     ADFDRFVRKEFGQRARTYAANSPERESFDRDVEKIEEYLANELRPESNGVAVFACAGA
                     GDFFEAAQFEAPVEHQRLYIYNQPHLYPLARLAGQFPRYAVLVADTNRARLFVFGRGK
                     TLKAEDVQNVKTRRSQVGGWSQMRYQRHLENYHLQHAKEVAEALERTLREDNIEHVIL
                     AGDEVIIPLLKEQFPKAVAEKVVDALSLPIDAPEHEILEASAEAMREHDAETDAAKVE
                     QLLNDYRAGGLAVVGSQDTLEALSMGQVEELIITARQQHVEGDREDVAILSDSEVVEP
                     VVAGADGAVANIDADSLIVAGDLVTRAQQTSARITFVEDPALLAEVGGVGAILRYRIE
                     PPTTDSAPQPQHA"
BASE COUNT          869 a         1956 c         1892 g          904 t
ORIGIN      
        1 tgtcggtcgt cggcgacgga cggctcgcgg gcgagttgcg ggagcgggcc gagaggctcg
       61 gcatcgccgg gcgcgtcgat tggcacgggg tcgtgcgcga cccgtacgcg ttctatcgcg
      121 cctcgtccat cttcgcgctg ccctcgcgcg tcgaggggac gccgaacgcc ttgctcgaag
      181 cgatgagctg cgggatgcct gtcgtcgtca gcgacggcgc gcccggcccg ctcgaactcg
      241 tcgaggacgg cgtgacgggt cttgtcgtcc cggtcaacga cacggcggcg ctcgccgcgg
      301 cgctcagccg cctcgcgcgc gacgaagagt tgcgtggccg cctcggcgaa gcggcgtgcg
      361 cgcgcgtctc ggagtacgac ctgccgcgcg cgctggccgc gtgggagtcg gccatcggcc
      421 tgacgaacca acaaaagccg tgaaccgtaa accgtaaacc gtgacgagtc agaacaggtc
      481 ttcactcgtt acggtttatg gtttacggtt cacggctttt gaggcgcggc gcgcggcgtc
      541 gtaagattcc tcgcggatgt caacacgccc atcttcgacg aggaggcacg ccgccccgcc
      601 cccaaagccg accctgcgcg agcgggtggc ggcgctgcgc tacgtcccgc ccttcgtccg
      661 gctcgtctgg cagacacatc gcgggatgac ggtggcgatg atggtgctca ggctcgtgcg
      721 cgccttcgtg ccggtcgcgg tgctctgggt cggcaagctc atcgtggacg aggtggtggc
      781 ggcgagtggg gctggccccg gcgcggcgga cttgtcgcgg ctgtggcgac tggtcgcagt
      841 ggagatcgcg ctcgcgctct tgggcgaggt gctggcgcgc gcctcttcgc tcgtcgagag
      901 tctgctcggc gacctcttct cgaaccacac gagcgtgcgg ctgatgagcc acgccgccac
      961 gctcgacctc taccagttcg agaacccgga cttctacgac cgcctcgacc gcgcgcgccg
     1021 ccagaccacc gggcgcatcg gcctgctcgc gcagctcctg tcgatggggc aggacgcgct
     1081 gacgctcctg agcttaggcg cggcgctcct cgtctacagc ccgtggctgc tcctgctgct
     1141 cgcggtcgcc gtgctgcccg gcttcctcgg cgaagcgcac tacgcggcgc tcgaatactc
     1201 tctgctctac cgctggacgc ccgagcgccg tcagctcgac tacctgcgac tcctgggcgc
     1261 gagcgaccgc ccggcgaaag aggtgcagat gttcggcctc gcgcgctggc tcgtcgagcg
     1321 ctaccgcgct ctctccgaca agttttacga ggagaataaa aggctctccg tgcgaaggag
     1381 cacggtcgct gcggctcttt cggccttcgg cgtcttgggc tactacggcg cctacgccgt
     1441 catcctcgcg cgcgccgtcg cgggcgccat ctcgctcggc acgctgacct tcctcgccgc
     1501 ctccttcctg cgcagccgcg acctcatcca gcgcctgctc tccggcgcga gcggcatcta
     1561 ccagcaatgc ctctacctca aagacctctt cgacttcttc gagacggagc cgcaaatcag
     1621 ctcgcgcgcg ggcgcgcccg cggtgccgcg cacactgcgc gaaggcttcc gtttcgagaa
     1681 cgtcggcttc cgctacccgg acagcgagcg ctggtctttg cgcggcgtca cgttccgttt
     1741 gcggcccggc gagaggctcg cgctcgtcgg cggcaacggg gccgggaaga cgacgctcgt
     1801 caagctgctc gcccgtctct acgacccgac cgaggggcgc atcctcttgg aaggccgcga
     1861 cctcagagac tacgacctcg cctctgtgcg ccgcagcgtc ggcgtcatct ttcaggactt
     1921 cttccgctac gacctccggc tcgacgagaa catcggcgtc ggcgagatcg aggacgtgcg
     1981 ccactacctc gacacggctg acgagatgca cgacgcctct catcaaaaac cgcgcggcga
     2041 tcgtgagaaa aaagcgggcg gcgacgacgg caacgcatca cgcgacgaag ggaacggcgc
     2101 gacgccgccc gcggcggtcg tcgcggcggc ggagaagtcg ctcgccgaca cgctgctcgc
     2161 gcgcttgccc gcgggctaca ggcagatgct cggtcgccgc ttcgagggcg gcgtcgatct
     2221 ctcgggcggc gagtggcaga aggtcgcgct cgcccgcgcc tacatgcgcg acgcgcacgt
     2281 gctcatcctc gacgagccga cctcggcgct cgacgcgcgc gccgagtacg aggtcttccg
     2341 gcgcttcgcc gagctgatgc gcggcaggat ggccgtcatc atctcgcacc gcttctcgac
     2401 cgtgcggatg gccgaccgca tcatcgtcct cgaagacggc gtcatcgtcg aagaaggcac
     2461 gcacgccgcg ctcgtcgaac gcggcggcct ctacgccgaa ctgttcgccc ttcaggccga
     2521 aggctatcgc tgacgcgtcc gcctcggcga ggctcacgcg ggagcgttgt cgatgaaggg
     2581 gccgctgccg gtcttccggc ggcgggcgac tccgctctta cgcgacgggg gattccgcgg
     2641 cgcgcgcgtc ggcgcaaccg ttcgcggctt cgggcagcag cgccgagagg tcttcgaggt
     2701 tgtctccgac ctgcccgccc gcgtcctgtc ccacttgcgc gagataactc cgaagcctgc
     2761 cgacgagtct ccgcagccgg gccgcgtctt cgcgcgcttc gcccagcgcg gctgactgcg
     2821 tgagcgcctt cttcagcgcg cggacggcgt agtgcagcgg gatcaagctg cccccgccgt
     2881 ccttcgcgcg cagcctctcg aactcctgct caaggcactc gttgagccgc gccacgtcgg
     2941 gcgcgtcccc gtactcctgc gccaggtagt agtagatgcg gtcgaactcc caccagctcg
     3001 cggcgcacct gcggagcgtc tcctcgcggg ggcgcgcgcc cttgcctttg ccgagctgga
     3061 acctttgctg ctgcgggtgg atgaggagcg ccttcttctc ctcgaacgtg tgcagccggg
     3121 cgaagcgccg cgactccgga agcaccgggc cgccgtccgc ggccagcagg cggacgacca
     3181 gttgccgtat ccgccggcgc cgctcctcgc acggccttct gacgccctcg cccaacgaca
     3241 ccttgccgtc cccgccgatc tcgaagtgct cctcgcggag caggagggcg gacatgtcca
     3301 tcgcgtagcc gtcgagctgg ccgaccagct cgtgatagag gtctgacagg tgcgagtcgt
     3361 cggcctccat cacctgcctg taacggctca accgctccag cgtctgccgc gcgccgtcgt
     3421 acctgtcgag ccatgtctgc cgcgtgcatt cgtcgagcgg ctcgttgaac gtcttgcgcc
     3481 ggaagagttg ttcgaggccg gggatgagtt cgtctatcgt gggcggcctg atcgcggcca
     3541 gccccttctc gaacgcgccc cagtggtggg cgaaggctct ctcgcacttc tcgcgcgcgt
     3601 ccgcgtccgc ccccatcgag gtgacgatgg cgtcgatcag cttttcggtg tcgtcgcggt
     3661 cgctccgcgt ggactggaac gcgccgaacg ggcctcgcag ctcgtccggt tcgacgttga
     3721 gcaggtagac gaagactttc ccccgccggt tcttcctgac ggcgcgggcg agcgcgcccg
     3781 tctcgaaatg aatccagccg gagtggagac tctcgggcgt cacgcagacg aggcccgcgt
     3841 cagccgcgtc gagggcgacg ctcacctcgt cgaaccagcg cgtgcccttc acgatgttct
     3901 ccgagaagaa ggggggcggc agatcaggga tgaaccgggg caggtactcg tacatcagct
     3961 tggcgagggc cttgcttctg tctcccgacc agcagatgaa gatgttcatg gcggtcgtcc
     4021 tttggcggcg aatcgggctg cgcgggggaa gcaaagtata acgcacgtgc ggcccgcgcg
     4081 gtcttttcgc gccgcgtgtg gccgccgaaa ttggagcgcg cggcgcgggc gtgctaatat
     4141 ccttcgcacc gattcccccg aatactcgat ggccggacgc aggcgcgcgc ggagtgtgcg
     4201 ctgtgcgcgg cttttagcga cgggagcgcc gacacgcgcg gcgtctcctt catcatgctt
     4261 tgaggtttcg tggattcgat attcgaaccg taaggaagaa gggggcagag aaacgagatg
     4321 gcacagagta aggaagcagt cgagggcggg acgctggacg atctgttgga gcggctggcg
     4381 gcgttcgagc cgacggggtt gccgttcatc agcctgtacc tgaacgcgca ggcgaacgag
     4441 aacgggcggg ccgacttcga ccgcttcgtc cgcaaagagt tcggccagcg cgcgcgcacc
     4501 tacgcggcga acagccccga gcgcgagagc ttcgaccgcg acgtggagaa gatcgaagag
     4561 tatctggcga acgagctgag gccggagtcg aacggcgtcg ccgtcttcgc ctgcgcgggc
     4621 gcgggcgact tcttcgaggc cgcgcagttc gaggctccgg tcgagcacca gcggctctac
     4681 atatacaacc agccgcacct ctacccgctc gcgcggctcg ccggacagtt cccgcgctac
     4741 gccgtgctcg tcgccgacac gaaccgcgcg cgcctcttcg tcttcggtcg cggcaagacc
     4801 ctgaaggccg aggacgtgca gaacgtcaag acgcgccgct cgcaggtcgg cggctggtcg
     4861 cagatgcgct accagcgcca cctcgaaaac taccacctgc aacacgcgaa ggaggtcgcc
     4921 gaagcgctcg aacgcaccct gcgcgaggat aacatcgagc acgtcatcct ggcgggcgac
     4981 gaggtcatca tcccgctcct caaggagcag ttcccgaagg cggtcgccga aaaggtggtg
     5041 gacgctttga gtctgcccat cgacgcaccc gagcacgaaa tcctcgaagc ctccgccgag
     5101 gccatgcgcg agcacgacgc cgagaccgac gccgcgaagg tcgagcaact gctgaacgac
     5161 taccgcgccg gcggactcgc cgtcgtcggc tcgcaggaca cgctcgaagc gctctcgatg
     5221 gggcaggtcg aggaactcat catcaccgcg cgccagcagc acgtcgaggg cgaccgcgag
     5281 gacgtggcca tcctctccga cagcgaagtg gtcgaacccg tggtggcggg cgccgacggc
     5341 gccgtcgcca acatcgacgc cgactcgctc atcgtcgcgg gcgatctggt cacgcgcgcg
     5401 cagcaaacct ccgcgcgcat caccttcgtc gaagaccccg cgctgctcgc cgaggtcggc
     5461 ggcgtcggcg ccatcctgcg ctaccgcatc gagccaccca cgaccgacag cgcgcctcag
     5521 ccgcagcacg cgtgagaagt caacgagcgg cgcgcggttg agtgtaccgg gaggccggat
     5581 tcgacgcaga ggacgcagag gcgcagagga cgcaaagaag a
//
LOCUS       DBNGOH010001039         2178 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_5107645, whole genome shotgun sequence.
ACCESSION   DBNGOH010001039 DBNGOH010000000
VERSION     DBNGOH010001039.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2178)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2178)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2178
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_5107645"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..497)
                     /locus_tag="AC159P_20185"
     CDS             complement(<1..497)
                     /locus_tag="AC159P_20185"
                     /inference="COORDINATES: protein motif:HMM:NF039409.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6328 family protein"
                     /protein_id="HIR3697818.1"
                     /translation="MAQLKDKVQNALDEGRILILGAHILLGFQLRSFLEKGFETLPVS
                     SRFLMLGGLGLLLIAVALLVAPTAYHRIVERGEDTEEIHSYTSRVMTFALLPLALALG
                     LDIYVAAAKIGGTTVGALAGVFASFAALFFWYGLEAVPRGGGGGGRVRATARKEEGEL
                     EIFDM"
     gene            complement(586..2124)
                     /locus_tag="AC159P_20190"
     CDS             complement(586..2124)
                     /locus_tag="AC159P_20190"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF004837.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HIR3697819.1"
                     /translation="MSERPDNLRALLEARAAAAPAKTFLFSEQDGRRFTYAEFDAAAD
                     ATARMLAARGVSKGDVVSLLLPNGAEYDVAYFACFKLGALAGPVNSLLKTEEMAYVVS
                     NSEAKVMLRGAEFEAQAAECAKTVGTLREVVPFDDVAAATVDFANVDERPSDVRLERN
                     DEAIIIYTSGTTGKPKGCLLTHGNLLANARQIVEWLGFTEDDRLLTVMPLFHMNAVTV
                     TTASALYAGGSTVVSPRFSASRFWQVVSDYGVTSFGSVATMLSMLLAKYPEGVPEGLD
                     TTRLRFAMCGSAPVPAEVMRRFEERFRCLVIEGYGLSESTCRSTFNPPDARRRPGSCG
                     VAIGNEMKVFDEEDREVADGELGEIVMRGENVFKAYYKNEEATRRAFRGGWFHTGDIG
                     SRDAEGFFHIVDRKSDMIIRGGENIYPREIDELLYKHESIAEAAAVGVPDELYGEEVA
                     AFVVLKEGRETNAEEIVEYCRAHLADYKCPKSVHFLDALPKGPTGKVLKRELVKSYRG
                     GPAA"
BASE COUNT          365 a          796 c          666 g          351 t
ORIGIN      
        1 ctcatgtcga aaatttccaa ctccccttcc tccttccgcg cggtcgcgcg cacgcgcccg
       61 ccgccgccgc cgccgcgcgg cacggcctcc agcccgtacc agaaaaagag cgccgcgaac
      121 gaggcgaaca cgcccgccag cgcgccgacg gtcgtccccc cgatcttcgc cgccgcgacg
      181 tagatgtcca gcccgagcgc gagcgcgagc ggcagcagcg cgaaggtcat cacgcgcgac
      241 gtgtaggaat gaatctcctc cgtatcctcg ccgcgctcga cgatgcggtg gtaggccgtc
      301 ggcgcgacga gcagcgcgac cgcgatcagg agcaggccca gcccgccgag catgaggaac
      361 cgcgacgaga cgggcagcgt ctcgaagccc ttttcgagga acgaacggag ctggaacccc
      421 agcaggatgt gcgccccgag gatgaggatg cgcccctcgt cgagcgcgtt ctgcaccttg
      481 tctttaagct gcgccaagtc tcttccggca cctctccccg cgagtttgtt tagagcgcgt
      541 cgaagaaatc agacatcgtg ccagcggccc cttcatgcgg cctcttcacg cggcggggcc
      601 gccgcggtac gacttcacga gttcccgctt caagaccttg ccggtcggcc ccttcggcag
      661 cgcgtcgagg aagtggacag acttcgggca cttgtaatcg gcgaggtgcg cgcggcagta
      721 ctcgacgatc tcctcggcgt tcgtttcgcg cccctccttg aggacgacga aggccgcgac
      781 ctcctcgccg tacagctcgt cgggcacgcc gaccgccgcc gcctccgcga ttgactcgtg
      841 tttataaaga agctcgtcga tctcgcgcgg gtagatgttt tcgccgccgc ggataatcat
      901 atccgatttg cggtcaacga tgtggaaaaa gccctcggcg tcgcgcgagc cgatgtcgcc
      961 cgtgtggaac cagccgccgc gaaaggcgcg ccgtgtcgcc tcttcgttct tgtaataagc
     1021 cttgaagacg ttctcgccgc gcatgacgat ctcgccgagc tcgccgtccg cgacttccct
     1081 gtcctcctcg tcgaagacct tcatctcgtt gccgatggcg acaccgcacg agccgggccg
     1141 gcgccgcgcg tccggcgggt tgaaggtcga gcggcacgtc gattccgaca gcccgtagcc
     1201 ttcgatgacg aggcaccgga accgctcctc gaaccgccgc atcacctcgg cgggcaccgg
     1261 cgccgagccg cacatggcga aacgcagccg cgtcgtgtcc aagccttcgg gcacgccctc
     1321 cgggtacttc gccagcagca tcgagagcat cgtcgccacc gacccgaacg aggtcacgcc
     1381 gtaatcgctc acgacctgcc agaaccgcga cgcggagaag cgcgggctga cgaccgtcga
     1441 gccgcccgca tagagcgcgc tcgccgtcgt caccgtcacc gcgttcatgt ggaagagcgg
     1501 catcaccgtc agcagccggt cgtcttccgt gaagccgagc cactccacga tctgccgcgc
     1561 gttcgccagc aggttcccgt gcgtcagcag acagcccttc ggcttccccg tcgtccccga
     1621 cgtgtagatg atgatcgcct cgtcgtttct ctccaaccga acgtcacttg gccgctcatc
     1681 gacgttcgcg aaatcgacgg tcgccgccgc cacgtcgtcg aacgggacga cttcacgcaa
     1741 cgtcccgacg gtcttcgcgc actcggcggc ctgcgcctcg aactccgcgc cgcggagcat
     1801 gaccttcgcc tccgagttcg agacgacgta ggccatctcc tccgtcttca gcagcgagtt
     1861 gaccggcccg gccagcgcgc ccagcttgaa gcaggcgaag taggcgacgt cgtactccgc
     1921 gccgttcggc aacagcaggc tcaccacgtc gcccttcgag acgccgcgcg ccgcgagcat
     1981 ccgcgcggtc gcgtccgcgg cggcgtcgaa ctcggcgtag gtgaaccgcc gcccgtcctg
     2041 ctcggagaac aggaaagtct tcgcgggcgc ggcggcggct cgcgcctcaa gcaacgcgcg
     2101 caggttatcg ggcctctcgc tcatcgtcgt tcagtcgggc ctatattcac caccgggacg
     2161 cgcagacgaa cagagggc
//
LOCUS       DBNGOH010001040         2415 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_5107690, whole genome shotgun sequence.
ACCESSION   DBNGOH010001040 DBNGOH010000000
VERSION     DBNGOH010001040.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2415)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2415)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2415
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_5107690"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1071)
                     /locus_tag="AC159P_20195"
     CDS             complement(<1..1071)
                     /locus_tag="AC159P_20195"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014673.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3697820.1"
                     /translation="MPLADRLKEKIGREGAITFREWMGAALYDEREGYYRRRAERWGR
                     AGDYRTSHERSRLFAATFARYFAALFVELGRPREFFVVEAGGGAGDFARGVLGTLRRD
                     APEVFHALRYVFDETSANSRRLAAAALAPFEERVEFRPLRDARAHFEKGIVFSNELLD
                     AFPVNRVRTRGGQLRELYVGVDEGGAFVWVEGELSDTMLANHFARAPLPEGHTAEVNL
                     EAEEWIARAAAALGAGFVVAVDYGDDAASLRAAPHRREGTLRAFSRHGFADDFLQRPG
                     EVDLTTTVDWTHVVEAGERAGLRTMLLERQDRFLLRAGLLEQLERETSAASDEAEVVR
                     LRLEAREMILPGGMAEHFHVLVQ"
     gene            complement(1190..1768)
                     /locus_tag="AC159P_20200"
     CDS             complement(1190..1768)
                     /locus_tag="AC159P_20200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697821.1"
                     /translation="MFCPKCGQRQAADDVRFCSSCGFTLNVVADVLAGGGQLHWRPPD
                     APATPQPLSPRQKGVRQGAMLMLSTLLVVPIVIFLGVALLNLPGVLIPLAGAVCIFGG
                     LLRILYALFLEEDRPALAPYATPTYVPPPSPPNYLGTPLQQNALPPPPRNAPAPSYRP
                     RYNTGELVERPGSVTENTTRLLDRKPDEPPKQ"
     gene            complement(1824..>2415)
                     /locus_tag="AC159P_20205"
     CDS             complement(1824..>2415)
                     /locus_tag="AC159P_20205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697822.1"
                     /translation="KEVRRTMYCPQCGQQQVTGEVRFCSRCGFQLEGVSGLLASGGAP
                     TLTAMQGAEWQESPRRKGARLGGKLILFGIFLIPALGILHEITKAPEEIMLFGVLTLL
                     AGFLRLIYAMIFEQGPFRQPRGHTQLVYAPTPPSHLAAPARGAAELPPAAAQPARAYV
                     PPRTDTAEISYRPSVTENTTRLLGEQEERDARRTRE"
BASE COUNT          367 a          777 c          896 g          375 t
ORIGIN      
        1 ctgcacgagc acgtggaagt gctcggccat gccgcccggc agaatcatct cgcgcgcttc
       61 gaggcggagg cgcacgactt cggcctcgtc actggccgcc gacgtttcgc gttcgagttg
      121 ttcgagcagc cccgcgcgga gcaaaaacct gtcctgcctt tcgaggagca tcgttcgcag
      181 ccccgcgcgc tcgcccgctt cgacgacgtg cgtccaatcg acggtcgtcg tcagatcgac
      241 ttcgccgggc cgctgaagaa agtcgtcggc gaagccgtgg cggctgaagg cgcgcagagt
      301 cccttcgcgc cggtgcgggg cggcgcgaag gctggccgcg tcgtcgccgt aatcgaccgc
      361 gacgacgaag cccgcgccga gcgcggcggc ggcgcgcgcg atccactctt cggcttcgag
      421 gttgacttcg gcggtgtggc cttcggggag cggcgcgcgc gcgaagtgat tggcgagcat
      481 cgtgtcggac aactcgccct cgacccagac gaaggcgccg ccctcgtcaa cgccgacgta
      541 gagttcgcgg agctggccgc cgcgcgtgcg gacgcggttg acggggaagg cgtcgagcag
      601 ctcgttggag aagacgatgc ccttctcgaa gtgcgcgcgc gcgtcacgga gggggcggaa
      661 ttcgacgcgc tcttcgaaag gggcgagggc cgccgcggca agtctgcgcg agttggcgct
      721 cgtctcgtcg aagacgtagc ggagcgcgtg gaaaacctcc ggggcgtcgc gccggagggt
      781 gccgagcacg ccgcgcgcga agtcgcccgc gccgccgccc gcctcgacga cgaagaactc
      841 gcgcggtcgc ccgagttcga caaagagggc ggcgaagtag cgcgcgaagg tcgcggcgaa
      901 gagacggctg cgctcgtgac tcgtccggta atcgcccgcg cggccccagc gctcggcgcg
      961 gcggcggtag tagccttcgc gctcgtcgta gagcgcggcc cccatccact cgcggaaagt
     1021 gatcgcccct tcccttccga ttttttcttt gagtctgtcg gcgagaggca taaagcagta
     1081 ggcagcaggc agtaggcagc aaaagcgaag acggtaggca cgaagccgac attacaagtt
     1141 cagctttatc attcaagctc cgaataaggg gttctttgct gcctactgcc tactgcttgg
     1201 gcggctcgtc aggtttccgg tcgaggagtc gcgtggtgtt ctcggtgacg ctgccgggtc
     1261 gctcgaccag ctcgcccgtg ttgtagcggg gccggtagga cggtgcgggg gcgttgcgcg
     1321 ggggcggcgg gagcgcgttc tgctgcaacg gcgtgccgag atagttcggc ggcgagggcg
     1381 gcgggacgta agtgggcgtg gcgtatggcg cgagcgccgg cctgtcctct tcgaggaaga
     1441 gtgcgtagag gatgcgcagc agcccgccga agatgcagac ggcgcccgcg agcggaatga
     1501 gtacgcccgg caggttgagc agcgccacgc cgaggaagat gacgatgggc acgacgagca
     1561 gcgtgctgag catcagcatc gcgccctggc gcacgccctt ctgccgcggc gagagcggct
     1621 gcggcgtggc gggcgcgtcg ggcggtcgcc agtggagctg accgccgccc gcgagcacgt
     1681 ccgcgacgac gttgagcgtg aagccgcagc tcgaacagaa gcgcacgtcg tcggctgcct
     1741 gtcgctgtcc gcacttgggg cagaacatgg gaagtatggc cctcgctatc tcggtcgcaa
     1801 cctcgcggtg agaagccccg cgcctattcg cgcgtgcggc gggcgtcgcg ctcctcctgc
     1861 tcgccgagca ggcgcgtggt gttctccgtc acgctcggtc ggtagctgat ctcggcggtg
     1921 tccgtgcgcg gcgggacgta ggcgcgcgcg ggctgcgcgg cggcgggcgg cagctcagcc
     1981 gcgccgcgcg cgggcgccgc cagatggctc ggcggcgtgg gcgcgtagac taactgcgtg
     2041 tgccctctcg gctggcggaa cggcccctgc tcgaagatca tcgcgtagat caggcggagg
     2101 aaaccggcga gcagggtgag cacgccgaag agcatgatct cctcgggcgc cttcgtgatt
     2161 tcgtgcagga tgcccagcgc cgggatgagg aagatgccga agaggatcag cttgccgccg
     2221 agccgcgccc ctttgcgccg cggcgactcc tgccactcgg cgccctgcat cgcggtcagg
     2281 gtcggggcgc cgccgctcgc cagcaggccg ctcacgcctt cgagttggaa gccgcagcgc
     2341 gagcagaaac gcacctcgcc cgtgacttgc tgttggccgc actgcgggca gtacatcgtt
     2401 cggcgaacct ctttt
//
LOCUS       DBNGOH010001041         2575 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3274477, whole genome shotgun sequence.
ACCESSION   DBNGOH010001041 DBNGOH010000000
VERSION     DBNGOH010001041.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2575)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2575)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2575
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3274477"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..474)
                     /locus_tag="AC159P_20210"
     CDS             complement(<1..474)
                     /locus_tag="AC159P_20210"
                     /inference="COORDINATES: protein motif:HMM:NF013056.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HIR3697823.1"
                     /translation="MISDSATNTDERKTSVRTELVAGVTTFFTMAYIVVVNPLILSTE
                     GTGMPAGGVLTATVLVCFTMTMLMGLYARLPFAVAPGMGINAFFAYTIILTQGVPWPV
                     ALGIVFWAGVLFLVVSLTPLREAIARAIPKNLRTATAAGIGVFLTFIGLQSAGLVA"
     gene            733..1953
                     /locus_tag="AC159P_20215"
     CDS             733..1953
                     /locus_tag="AC159P_20215"
                     /inference="COORDINATES: protein motif:HMM:NF015338.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HIR3697824.1"
                     /translation="MNIATCEKARPAPALEHPLRPHFDALARFDSTKALEASALYRQL
                     RPEIEALLDSVACESFAPPRTPSNRARGGAPVRATAWNIERGSRLEGIVEVLGAHPLL
                     SESDVLLLTELDYGMARSGNRHVAREIAERLGWGYAFAPCYVNLAKGSGLESEAAGEN
                     REALHGNAVLSRYPLARAHSVALPNGKDKMRGKEKRLGCQRAVVADVEHPAGAVRVVS
                     LHLDAHSSQRHRQRQMRVVLDHLERLEPSLPVLVGGDWNTSTYNSSRAVYSIFGFFRR
                     VLMGVRHVIRNHYVYPERWFERRLFRELEARGFAFRELNEPGAGTLHYDVQDLAANTN
                     MADWVPRWCFWWIDWALKENDGRCSLKLDWFAGRGLAPDPANPPRVVGNLRDRDGGVL
                     SDHDAIVVDFIPRQ"
     gene            complement(2132..>2575)
                     /locus_tag="AC159P_20220"
     CDS             complement(2132..>2575)
                     /locus_tag="AC159P_20220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018479990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697825.1"
                     /translation="RSMLKALGSGLAGALALTLVHETARRLRDDAPRMDVLGMRAIAK
                     TLRAADVEQPDVELRDLALAGDLAANSLYYSLVGFGKREDALLCGAALGLAAGLGAVT
                     LPGPLGLGPQPTERPPATQLMTVTWYLLGGLAAGAAYLALTSDEE"
BASE COUNT          425 a          869 c          875 g          406 t
ORIGIN      
        1 ggcgacgagt cccgcgcttt gcaggccgat gaaggtcagg aagacgccga tgccggcggc
       61 ggtggccgtc cgcaggttct tcgggatggc gcgcgcgatg gcctcgcgca gaggcgtcag
      121 ggagacgacg aggaagagca cgcccgccca gaagacgatg ccgagcgcga ccggccacgg
      181 cacgccctgc gtgaggatga tcgtgtaggc gaagaaggcg ttgatgccca tgccgggcgc
      241 gacggcgaac gggaggcgcg cgtagaggcc catcagcatc gtcatcgtga agcagacgag
      301 cacggtcgcc gtcagcacgc cgccggcggg catccccgtc ccctcggtcg agagaatgag
      361 cgggttgacg acgacgatgt aggccatcgt gaagaaggtc gtcacgcccg cgaccagctc
      421 cgtgcggacg cttgtcttgc gctcgtcggt gttggtggcg gagtccgaga tcatagttga
      481 gcttgattct ttggcgttga tgaccggggg agaaatcttt agacgcagag gacgcggagg
      541 ggcgcggagg gcgcagagca gaaagggcga cggcgagctg tcataattga atcgggtttc
      601 tctgcgtcct ccgcgcccct ccgcgtcctc tgcgttgaaa cagggcgcgc cctacgcgca
      661 acgcacgaag gaagcgcggg cggccccggt tgaaaagaac gcggcgcaga ctgtaacatc
      721 tccgcctttc cgatgaacat agcgacctgc gaaaaagcac gccccgcgcc cgcgctcgaa
      781 catcccttgc gcccgcactt cgacgccctc gcgcggttcg attcgacgaa ggcgctcgaa
      841 gcctccgcgc tctatcgaca actccgcccc gagatcgaag cgcttctcga cagcgtcgcg
      901 tgcgagtcgt tcgcgccgcc gcgcacgccc tcgaatcgcg cgcgcggggg cgcacccgtc
      961 cgcgcgacgg cgtggaacat cgagcgcggc tcgcgcctcg aaggcatcgt cgaagtgctc
     1021 ggcgcgcacc cgctgctctc ggagagcgac gtgctgctgt tgaccgaact cgactacggg
     1081 atggcgcgtt cgggcaaccg ccacgtcgcg cgcgaaatcg ccgagcgcct cggttggggc
     1141 tacgccttcg cgccgtgcta tgtgaatctg gcgaagggga gcggccttga gagcgaggcc
     1201 gcgggcgaga accgcgaggc gctgcacggc aacgccgtac tgtcgcgtta cccgctcgcc
     1261 cgcgcgcact ccgtcgcgct gccgaacggc aaggacaaga tgcgcggcaa ggagaagcgt
     1321 ttgggttgcc agcgcgcggt cgtcgccgac gtggagcacc ccgcgggcgc ggtgcgagtc
     1381 gtgagcctgc acctcgacgc gcattcgagc cagcgccatc ggcagcggca gatgcgcgtc
     1441 gtgctcgacc acctcgaacg acttgagcct tccttgcccg tcctcgtcgg cggcgactgg
     1501 aacacctcga cctacaattc gagccgcgcc gtctactcca tcttcggctt cttccgccgc
     1561 gtcctgatgg gcgtccgcca cgtcatccgc aaccactacg tctaccccga gcgctggttc
     1621 gagcgcaggc tgttccgcga gcttgaagcg cgcggcttcg ccttccgcga gttgaacgag
     1681 ccgggcgcgg gcacgctcca ctacgacgtg caagacctcg ccgccaacac gaacatggcc
     1741 gactgggtgc cgcgctggtg cttctggtgg atcgactggg cgctcaaaga gaacgacggg
     1801 cgctgctcgc tcaagctcga ctggttcgcc gggcgcggtc ttgcgcccga ccccgcgaac
     1861 cccccgcgcg tcgtcggcaa cttgcgcgac cgcgacggcg gcgtgctctc ggatcacgac
     1921 gccatcgtcg tcgatttcat cccgcgacaa tgaagaagta ggcagtaggc agcaggcagc
     1981 aggcagttaa gaaaagaagg gcgaagatgc ctgagcacgt tatctctcaa gcatcttcgc
     2041 ccttctactt ttttaactgc ccgctgcctg ctgcctgctg cctgcttttc ttgactgcct
     2101 accgcctact gcctactgtt ctttgaggcg cttactcttc gtccgatgtg agcgcgaggt
     2161 aggccgcccc cgcggcgagg ccgccgagga gataccatgt gacggtcatg agctgcgtgg
     2221 cgggcgggcg ctcggtgggc tgcgggccga ggccgagcgg gccgggcagc gtgacggcgc
     2281 ccagccccgc ggccagtccg agcgcggcgc cgcagagcag ggcgtcttcg cgcttgccga
     2341 agccgacgag gctgtagtag agcgagttgg cggccagatc gcccgcgagc gcgaggtcgc
     2401 gcagctccac gtcgggctgc tccacgtccg ccgcgcgcaa agtcttggcg atggcgcgca
     2461 tgccgagcac gtccatgcgc ggtgcgtcgt cccgcaaccg ccgggccgtt tcatgtacca
     2521 gcgtgagcgc cagcgccccc gccagaccgc tcccgagtgc cttcagcatc gaccg
//
LOCUS       DBNGOH010001042         3305 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1441915, whole genome shotgun sequence.
ACCESSION   DBNGOH010001042 DBNGOH010000000
VERSION     DBNGOH010001042.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3305)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3305)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3305
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1441915"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            108..320
                     /gene="rpmD"
                     /locus_tag="AC159P_20225"
     CDS             108..320
                     /gene="rpmD"
                     /locus_tag="AC159P_20225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013569705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HIR3697826.1"
                     /translation="MAEENNQQGGAGGGKIRIQYYRSSIGFPKTQKQVVKSVGFTKLN
                     QTIERPDTPSMRGVVAKVPHLLRIIE"
     gene            416..904
                     /gene="rplO"
                     /locus_tag="AC159P_20230"
     CDS             416..904
                     /gene="rplO"
                     /locus_tag="AC159P_20230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HIR3697827.1"
                     /translation="MGLNNLHPAKGSTHKKKRLGRGPGSGLGKTAGRGNKGQKSRSGY
                     SMKIGFEGGQMPLQRRLPKRGFTNIFKKQWIEVSLATLDASFAAGDSVTPELMHERGV
                     IAKGHRDVVVLGTGELTKALTIFAHRFTKGARAKIEAAGGTVNVVSSLHKADALVPVE
                     PQ"
     gene            1149..2567
                     /gene="secY"
                     /locus_tag="AC159P_20235"
     CDS             1149..2567
                     /gene="secY"
                     /locus_tag="AC159P_20235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HIR3697828.1"
                     /translation="MDKFFAAISNMFRVPDLRKRVLFTLGLLAVYRFGAHVTTPGIDQ
                     DQLRRVWDEVGSTLLGVLDLFSGGNFKTISVFALGVTPYITASIILQLMTTVFPQLKK
                     LQEEGEAGRQKINQYTRYLTVVLALFQTFIVARWLQANAVGDDTWGFIVTTMITLTTG
                     TIFVMWLGEQITERGIGNGISLLIFAGIVIGLPSGVQQVFGKLRSGDAFEAIGVIALV
                     VVLLALIAFVVFVETARRKVPISYAKRHVGRQLVGGQQTHMPIKINMGGVIPVIFASS
                     VLTIPQTVMQFVQPDMNNPQALSTRVFTFFQSFHGGDPYYELLFTFLIVVFTFFYITI
                     IFNVEEVADNLRKHGGFIPGIRPGRNTAEFLNTILTRLTTVGAIYLALVALIPQIMLS
                     GFNVGRLPFVGVGLEDFVSRTPGLGWIPKGMGYQFYFGGTSLLILVGVAMDTVAQIEA
                     QLVMRNYEGFFGPGSRLRGRRA"
     gene            2657..>3305
                     /locus_tag="AC159P_20240"
     CDS             2657..>3305
                     /locus_tag="AC159P_20240"
                     /EC_number="2.7.4.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase family protein"
                     /protein_id="HIR3697829.1"
                     /translation="MSKIIVPIGAPGAGKGTQARLIQERLGLPQISTGDMFRAMKEAR
                     TPLAAEVRAILSSGALVPDEITARVVEERTSREDARGGYVLDGYPRNAAQAETLEKLA
                     REQGHEIVPVYVDVPFDILEKRMTGRRTCPVCGEIYNVYFKPPKSDEVCDLHPTVKLV
                     RRADDNEETARRRKGVYEQETLPLVAYYEQSGRLRRVDGTRDPEAIYVDVEKIVND"
BASE COUNT          631 a         1047 c         1041 g          586 t
ORIGIN      
        1 agcaggtgga tgaactgtaa agaagtaaat agtgaatggt gaatggtgca tagacaaggc
       61 ttttccccat tcactgttga ccattgacca ttcacgaggt ttcagaaatg gctgaagaga
      121 ataaccagca gggcggcgcg ggcggcggca agattcgcat ccagtattac cgcagctcca
      181 tcggcttccc gaagacgcag aagcaggtcg tcaagagcgt cggattcacg aagctcaacc
      241 agaccatcga gcgccccgac acgccctcga tgcgcggcgt cgtcgccaaa gtcccgcacc
      301 tgttgcggat cattgaataa gagccgtcaa cggtaaaccg tcaaccgtga caagtcggaa
      361 cggcttccca cttatcacgg ttcacggttc acggcgtttg aggtttgaaa tggctatcgg
      421 tttgaacaac ctgcacccgg cgaagggttc gacgcacaag aagaaacgtt tgggccgcgg
      481 ccccggctcg ggtctgggca agacggcggg gcgcggcaac aagggccaga agtcgcggtc
      541 gggctacagc atgaagatcg gcttcgaggg cgggcagatg cccttgcagc ggcggctgcc
      601 gaagcgcggc ttcaccaaca tcttcaagaa gcagtggatc gaggtcagcc tcgccacgct
      661 cgacgcgagc ttcgcggcgg gcgactcggt gacgccggag ctgatgcacg agcgcggcgt
      721 catcgccaag gggcaccgcg acgtggtcgt gctcggcacg ggcgagctga cgaaggcgct
      781 caccatcttc gcgcaccggt tcacgaaggg cgcgcgcgcg aagatcgagg ccgcgggggg
      841 gacggtcaac gtcgtctctt cgctccacaa ggcggacgcg ctcgtgccgg tcgagccgca
      901 gtaaggctcc cggcgttgtt gaaagtcggg caggaggacg cgctgaagac gccgcgagga
      961 agaccatcga ggtctcttca gcgtcttcag cgttgtttgc taatgagctg ttagctgtta
     1021 gccgttagct tttagctttc agccatacac gggttattat tatcctctga gccggggaag
     1081 gtagcgctga ccttcgacag aaaagttgaa gctaacagct gatagctaac agctaagagc
     1141 caatcatcat ggataagttt ttcgccgcca tcagcaacat gttccgcgtg cccgacctgc
     1201 gcaagcgcgt cctgttcacg ctcggactgc tggccgtcta ccgcttcggc gcgcacgtca
     1261 ccacgccggg catcgaccag gatcaactcc gccgcgtctg ggacgaggtc ggcagcaccc
     1321 tgctcggcgt gctcgacctc ttctccggcg gcaacttcaa gacgatctcg gtcttcgccc
     1381 tcggcgtcac gccctacatc accgcctcga tcatcctcca gttgatgacg accgtcttcc
     1441 cgcagctcaa gaagttgcag gaggagggcg aggccgggcg ccagaagatc aaccagtaca
     1501 cgcgctacct gacggtcgtg ctggccctgt tccagacctt catcgtcgcg cgctggctcc
     1561 aggccaacgc cgtcggcgac gacacgtggg gcttcatcgt gacgacgatg atcacgctca
     1621 ccaccggcac catcttcgtg atgtggctcg gcgagcagat caccgagcgc ggcatcggca
     1681 acggcatctc gctgctgatc ttcgccggca tcgtcatcgg cctgccgagc ggcgtccagc
     1741 aggtcttcgg caagctccgc agcggcgacg ccttcgaggc catcggcgtc atcgcgctcg
     1801 tcgtcgtgct gctcgcgctg atcgccttcg tcgtcttcgt cgagacggcg cggcgcaagg
     1861 tgccgatctc ttacgccaag cgccacgtcg ggcgccagct cgtcggcggg cagcagacgc
     1921 acatgcccat caagatcaac atgggcggcg tcatccccgt catcttcgcc tcgtcggtgc
     1981 tgacgatccc gcagaccgtc atgcagttcg tccagcccga catgaacaac ccgcaggcgc
     2041 tctcgacgag ggtcttcacc ttcttccaga gcttccacgg cggcgacccc tactacgagc
     2101 tgctgttcac gttcctgatc gtcgtcttca ccttcttcta catcacgatc atcttcaacg
     2161 tcgaagaggt ggccgacaac ctgcgcaagc acggcggctt catccccggc atccggcccg
     2221 gcaggaacac ggcggagttc ctcaacacga tcctgacgcg gctcacgacg gtcggcgcca
     2281 tctacctcgc gctcgtcgcg ctgatcccgc agatcatgct gagcggcttc aacgtcgggc
     2341 ggctcccgtt cgtcggcgtc ggcctcgaag acttcgtcag ccgcacgccc ggattggggt
     2401 ggattcccaa gggcatgggc tatcagttct acttcggcgg cacctcgctc ctgatcctcg
     2461 tcggcgtggc gatggacacc gtcgcgcaga tcgaggcgca gctcgtcatg cgcaactacg
     2521 aaggcttctt cgggccgggc agccgcctgc gcggtcggag agcgtaaaaa cagaggccag
     2581 aggtcggagg tcggaggtca gtggaaactg attgctttta ctgacctccg acctctgacc
     2641 tccgacctct gtcgttatgt cgaagatcat cgtcccgata ggcgcgccgg gcgcgggcaa
     2701 ggggacgcag gcgcggctga ttcaggagcg cctgggtctg ccgcagattt cgacgggcga
     2761 catgttccgc gcgatgaagg aggcgcggac gccgctcgcc gcagaggtgc gggcgatcct
     2821 gtcttcgggc gcgctggtgc cggacgagat cacggcccgc gtcgtcgagg agcggacgag
     2881 ccgcgaagac gcccgcggcg gctacgtgct cgacggctac ccgcgcaacg cggcgcaggc
     2941 agagacgctc gaaaagctgg cgcgcgagca ggggcacgag atcgtccccg tttacgtgga
     3001 cgtgcctttc gacattctgg agaagcggat gacggggcgg cgcacctgcc cggtctgcgg
     3061 cgagatttac aacgtctact tcaagccgcc gaagtcggac gaggtctgcg accttcaccc
     3121 cacggtgaag ctcgtccgcc gcgccgacga caacgaagaa acggcccgga ggcgcaaagg
     3181 agtttacgag caggagacac tgccgctcgt cgcctattac gagcagtccg ggcgcctgcg
     3241 ccgcgtagac ggcacacgcg atccggaggc gatttacgtg gacgtggaga agatagtgaa
     3301 tgatg
//
LOCUS       DBNGOH010001043         3296 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3274548, whole genome shotgun sequence.
ACCESSION   DBNGOH010001043 DBNGOH010000000
VERSION     DBNGOH010001043.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3296)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3296)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3296
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3274548"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1208
                     /locus_tag="AC159P_20245"
     CDS             <1..1208
                     /locus_tag="AC159P_20245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014101067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HIR3697830.1"
                     /translation="YNFADTTEVFTTHYTVKKAVIAPGKYRKITGNEATAIGFIAAAE
                     LAGQPLFYGSYPITPASDILHELSRHKNFGVKTFQAEDEIAAVCAAIGASFTGHVGVT
                     GTSGPGVALKQEAIGLAVMTELPLVIINVQRGGPSTGLPTKTEQADLFQAVWGRNGEC
                     PAIVVAPATPADCFHMAIEAVRLAFKYMTPVFYLSDGYLANGAEPWLVPGIDSLPKIE
                     LKFADDPANFLPYSRDEETLARPFALPGTPGLEHRVGGIEKQHLTGNVNYDPENHHFM
                     VTLRQQKVDRAAQDVPPVEVFGDPTGKVLVLGWGSTYGSITSAVEKLRQEGKPVSSAH
                     LRHLNPFPSNLGDVLRSFDTVVVPEMNLGQLCTMIRAKYLVNAVAFSKVKGRPFQIRE
                     IVREVEKYL"
     gene            1231..1866
                     /locus_tag="AC159P_20250"
     CDS             1231..1866
                     /locus_tag="AC159P_20250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015243783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697831.1"
                     /translation="MTASAANVDARSLLGAYAEGLRYFMGEGALNTALAQVADDLRRH
                     GIDYMVIGAVAMLAHGYPRFTEDIDLVLTAEGLDAFHRELVGLGYVPAFEGARKRLRS
                     TKGNVPVEVIVAGEYPGDGKPKPVSFPVPSDASVEIDGVRVVTLEKLIELKLASGMTA
                     PDRLKDLADVQELIKARGLSADFAGQLNPYVRGKFSELCESVERGRRGGEE"
     gene            1908..3065
                     /locus_tag="AC159P_20255"
     CDS             1908..3065
                     /locus_tag="AC159P_20255"
                     /EC_number="1.2.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006140990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HIR3697832.1"
                     /translation="MSSNGQGNGNGAQPPVEEAPQGIGGNVSGSGPSPESEPGSVVAG
                     KAAPPPGKLTKADFSSDQEVRWCPGCGDYAILNNVQKVMPELGIPREKIVFVSGIGCS
                     SRFPYYMNTYGFHSIHGRAPAIATGIKTANPELSVWVITGDGDALSIGGNHFMHAIRR
                     NLDLNYILFNNRIYGLTKGQYSPTSEFGKKTKSTPMGVIDYPVNPLSLAIASEATFVA
                     RSIDIDVKHLGAIVQAAARHKGISFVEVYQNCNIFNDGAFDSFAERTARPDNVLYLEH
                     GQPMVFGKNRERGIRMRGTRPEVIQLNAETGLTESDCLVHDAHERDTSVAFMLARMEY
                     PDFPQPVGIFRSVERATYEDLLAGQIEAAVAKQGRGNLDKLINSGETWVVE"
BASE COUNT          594 a         1166 c         1017 g          519 t
ORIGIN      
        1 gctacaactt cgccgacacg accgaggtct tcaccaccca ctacacggtc aagaaggccg
       61 tcatcgcgcc cggcaagtac cgcaagatca cgggcaacga ggcgaccgcc atcggcttca
      121 tcgccgcggc ggagctggcg ggccagccgc tcttctacgg ctcctacccg atcacgcccg
      181 cctccgacat cctgcacgag ctgtcgcggc ataagaattt cggcgtcaaa acctttcagg
      241 ccgaagacga gatcgccgcc gtctgcgcgg ccatcggcgc ctctttcacc ggccacgtcg
      301 gtgtcacggg cacgagcggc cccggcgtcg ctttgaagca ggaggccatc ggcctcgccg
      361 tgatgaccga gcttccgctc gtcatcatca acgtccagcg cggcggcccc tcgacaggct
      421 tgccgacgaa gaccgagcag gccgatctgt tccaggccgt ctgggggcgc aacggcgagt
      481 gcccggccat cgtcgtcgcg ccggccacgc ccgccgactg cttccacatg gcgattgagg
      541 ccgtcaggct cgccttcaag tacatgacgc cggtcttcta tctttccgac ggctacctcg
      601 ccaacggcgc cgagccgtgg ctcgtgcccg gcatcgacag cctgccgaag atcgagctga
      661 agttcgccga cgaccccgcg aacttcctgc cctactcgcg cgacgaagag acgctcgcgc
      721 gccccttcgc gctccccggc acgcccggcc ttgagcaccg tgtcggcggc atcgagaaac
      781 agcatctcac gggcaacgtc aactacgacc ccgagaacca ccacttcatg gtcacgctcc
      841 ggcagcagaa ggttgaccgc gccgcgcagg acgtgccccc tgtggaagtc ttcggcgacc
      901 ccacgggcaa ggtgctcgtg ctcggctggg gttcgaccta cggctcgatc acttcggcgg
      961 tcgagaaact ccgtcaggaa ggcaagcccg tgtcgagcgc gcacctgcgg cacctcaacc
     1021 ccttcccctc gaacctcggc gacgtgctcc gcagcttcga caccgtcgtc gtccctgaga
     1081 tgaacctcgg ccagctctgc accatgatcc gcgcgaagta tctggtcaac gccgtcgcct
     1141 tctccaaagt gaagggccgc ccgttccaga tccgcgagat cgtgcgcgag gtcgagaagt
     1201 acctgtaaac gaaaccgggt gcagtaagcc gtgaccgctt ccgccgcaaa cgttgacgct
     1261 cgttcgctcc tcggcgcata cgccgagggg ctgcgctact tcatgggaga aggagcactg
     1321 aacaccgcgc tcgcgcaggt ggccgacgac ttgcggcggc acgggatcga ttacatggtg
     1381 attggcgccg tcgccatgct ggcccacggc tacccgcgct tcacggaaga catcgacctc
     1441 gtgttgacgg cggaagggct ggacgcgttc caccgcgagt tggtcggtct cgggtacgtg
     1501 cccgcattcg agggcgcgcg caagcgtctg cgctcgacga agggcaacgt gcccgtcgag
     1561 gtcatcgtcg cgggcgagta ccccggcgac ggcaagccga agccggtgag tttccccgtc
     1621 ccttcggacg cctcggtcga aatcgacggc gtgcgcgtcg tgacgctcga aaagttgatc
     1681 gagctgaaac tcgcctccgg catgaccgcg cccgaccgcc tcaaggactt ggcggacgtg
     1741 caggagttga tcaaggcgcg cggcctctcc gcggacttcg cggggcaact gaacccttac
     1801 gtgcggggaa agttttcgga gttgtgtgag tccgtcgagc gcggcaggcg cggcggcgag
     1861 gagtgagaga cgaagcggat tcggattcat tcgagaggaa tcaagagatg agcagcaacg
     1921 gacagggcaa cgggaacggg gcgcagccgc cggtcgagga ggcgccgcag ggcatcgggg
     1981 ggaacgtgtc ggggagcggg ccgtcgcccg agagcgagcc gggttcggtc gtcgcgggta
     2041 aagccgcgcc gccgccgggc aagctcacga aggccgattt ctcttcggat caggaggtgc
     2101 gctggtgccc aggctgcggc gactacgcga tcctgaacaa cgtgcagaag gtgatgcccg
     2161 aactcggcat cccgcgcgag aagatcgtct tcgtgtcggg catcggctgc tccagccgct
     2221 tcccctacta catgaacacc tacggcttcc actcgattca cggtcgcgcg cccgcgatcg
     2281 cgaccggcat caagaccgcg aacccggaac tctccgtgtg ggtcatcacc ggcgacggcg
     2341 acgccttgag catcggcggc aaccacttca tgcacgccat ccgccgcaac ctcgacctca
     2401 actacatcct cttcaacaac cgcatctacg gcctcacgaa ggggcagtac agtccgacct
     2461 cggagttcgg caagaagacc aagtccacgc cgatgggcgt catcgactac ccggtcaatc
     2521 ccctctcgct cgccatcgcc tcggaggcga ccttcgtggc gcgctccatc gacatcgacg
     2581 tgaagcacct cggcgccatc gtccaggcgg cggcccggca caagggcatc agcttcgtcg
     2641 aggtctacca gaactgcaac atcttcaacg acggcgcgtt cgatagtttc gccgagcgca
     2701 cggcgcggcc cgacaacgtg ctctacctcg aacacggcca gccgatggtc ttcggcaaga
     2761 accgcgagcg cggcatccgc atgagaggca cgcgccccga agtcatccag ctcaacgcgg
     2821 agacgggcct caccgaatcg gactgcctcg tccacgacgc gcacgagcgc gacacctcgg
     2881 tcgccttcat gctcgcgcgg atggagtacc cggacttccc gcagccggtc ggcatcttcc
     2941 gctcggtcga gcgcgcgacc tacgaagacc tgctcgccgg acagatcgag gcggcggtcg
     3001 ccaagcaagg gcgcggcaac ctcgacaagc tcatcaacag cggcgagacg tgggtcgtcg
     3061 aataaccgcg cgcctccctt tcggacggtc aaccgctcgc gcccgcccgc aggccgcgcc
     3121 cgaaacgtgt cgcacaaccg tttcggcgcg gccttcgtat atccgccgaa ctctcgaagg
     3181 acaacccggg ggtttccccc ttgctctctc agcgccgcct ctcccttttc gccctccctc
     3241 tgctgctcct cgcgctctct gccggggcgc aggcgcaaca gcccgaactg gtcgtg
//
LOCUS       DBNGOH010001044         6771 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3458020, whole genome shotgun sequence.
ACCESSION   DBNGOH010001044 DBNGOH010000000
VERSION     DBNGOH010001044.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6771)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6771)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6771
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3458020"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..486)
                     /locus_tag="AC159P_20260"
     CDS             complement(<1..486)
                     /locus_tag="AC159P_20260"
                     /inference="COORDINATES: protein motif:HMM:NF024110.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3697833.1"
                     /translation="MKPLLKIIDSRFWSLFMKELRQIKRNRKLVMMLVVPPTVNLVLL
                     GFAMNPEVKDLRLGVVDESRTAESRTLVSAFVESRSFIVAGQYASGAELGRAISAGEL
                     DAGLIVPHDFARTRAKGQAAEVQIIVDSVNSNTAGIAAGYAERVIGALNRKLAASAGA
                     PL"
     gene            complement(486..1418)
                     /locus_tag="AC159P_20265"
     CDS             complement(486..1418)
                     /locus_tag="AC159P_20265"
                     /inference="COORDINATES: protein motif:HMM:NF024110.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3697834.1"
                     /translation="MSRRYAEALRASLTFRVVPLEAGERPENALEDETAHAAVVIPEG
                     FDRDLRRGLGAEVQWLLDGTDANTANIMRGHAAAITQAFNAEVAPARERGSIVPAVRF
                     WYHPGRDSDKYIGPGIFAVGLALFPPLLAALAMSREGEQKTILQVYVSSISAHEYLLG
                     KILAFGIIAAAEWVLTLVFAMFLFGLGFKGDPTPFVVSSLLYLFCVVAFGAMIGARIP
                     DRAAAIQAIQNTGFLLSYLLAGFIFPISNIPEGVRWISYLIPARYYIEVSRDVFVRGG
                     GWPAVWNAPVALGLLGAFFFLSAWKRMRSMQVEA"
     gene            complement(1379..2440)
                     /locus_tag="AC159P_20270"
     CDS             complement(1379..2440)
                     /locus_tag="AC159P_20270"
                     /inference="COORDINATES: protein motif:HMM:NF024583.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HIR3697835.1"
                     /translation="MSDPAKSPAPGSMAEQFESLLGVTESIISHRNLAELFHDLAAQL
                     RPLVSFDYINVLLHDEARGVMRLHVMERRDPDGLPAGYEVPVEGTGAGWVWQTQQPLI
                     VHDVDEERRFPFTTDLFRRYGIHSFYSFPLTSAGRRLGTMGFGCQERHGCDAESLAFL
                     EQVVKQVAIAVENALNFESATRERARAELLLDVNNAVVSHLSLRDLLLNISTHISRNV
                     HHDLASVLLYDEATGQLRVHALDAPAPGGVLAEGSVLPMEGTPPGLAIKTRATVRRER
                     IDLAEFHSPVMRLAYEAGLRSGCSVPLVSHDRVLGTINVGSLREAAFTEADAELLGQI
                     ANQIAIGCRAATRRPCARR"
     gene            complement(2809..4464)
                     /locus_tag="AC159P_20275"
     CDS             complement(2809..4464)
                     /locus_tag="AC159P_20275"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008506892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HIR3697836.1"
                     /translation="MKLESLSKRSCLLVLSLIVWPCASAAQTAPAAGAGGAVSKVWVA
                     DNGDGTYKNPVLHADYSDPDAVRVGDDYYMTSSSFNAAPGLPILHSKDLVNWRLVGHV
                     FREQPPYDVFRKTQHGGGVWAPSIRHHNGEFYIYYPDPDRGIYMVKAKDAAGPWSEPL
                     LIKEAKGWIDPCPLWDEDGNAYLVSATAASRSGIKSILVVSRMSADGTKLLDDPVIVF
                     DGHEKHPTVEGPKFHKRNGFYYIFAPAGGVEQGWQLVLRSRNVYGPYEERIVLAQGKT
                     KINGPHQGAWVETQTGESWFLHFQDKGPYGRIVHLQPVRWVNDWPVMGADADGDGTGE
                     PVLKFKKPNVGKSYPTETPPESDEFDSTRLGLQWQWFANPQTNWMFLGGRHGYLRLLN
                     VPLPEGFKNFWDVPNILAQKFPAPAFTATTKVTFTARHDEEKAGLIVTGLDYAYLSLK
                     KKGEQLFVSQTVCKDADKGGPEREGAALPVKGDTFYLRVKVAEGAVCTFSYSADGKTF
                     TTVGEPFTARQGRWIGSTLGLFAVRTGTAREVGYADFDWFRVE"
     gene            complement(4533..5303)
                     /locus_tag="AC159P_20280"
     CDS             complement(4533..5303)
                     /locus_tag="AC159P_20280"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR3697837.1"
                     /translation="MIVTGGSRGIGEGCARVFAEAGALVVIASRDESEGAALAAELNA
                     RGPGGAAHFVACDVTKAADLEHLVAETVHRAGRIDCLINNAGWHPPHKPIDDFSLEEF
                     RELLELNLVSVFAACKLALPHLRRTRGNIINMSSLVAQIGQLHGTTYVATKGAVTAFT
                     KALAIDEAAHGVRVNSVSPGNIYTPLWQQAIDAAPDPARWRAAGEAAQLVGRMGTAEE
                     AGRLCLFIAAEATFTTGVDHVISGGAELGYGRKDRVEG"
     gene            5655..5891
                     /locus_tag="AC159P_20285"
     CDS             5655..5891
                     /locus_tag="AC159P_20285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697838.1"
                     /translation="MKGLDIPLWAVGIAAAAYALYEFMRFATATDPATGFQDMWAGVN
                     HLYVGLAAAVVAAVCIVWAFVRRPHEMEEIHVTK"
     gene            6161..6358
                     /locus_tag="AC159P_20290"
     CDS             6161..6358
                     /locus_tag="AC159P_20290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697839.1"
                     /translation="MNPERIGKADEVFRAALDPASGPSAAYLVEACAGDEELRREVES
                     LLSSRWGLCRGRLPVASSPSR"
     gene            complement(6418..6642)
                     /locus_tag="AC159P_20295"
     CDS             complement(6418..6642)
                     /locus_tag="AC159P_20295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697840.1"
                     /translation="MFKLISTTLVLCALSAGALRAQTPQGATTQQQDRQAQEQAERDR
                     KQAEREQKEKDKDARKEPGTRCGRWKRGKA"
BASE COUNT         1071 a         2287 c         2244 g         1169 t
ORIGIN      
        1 aaggggcgcg cccgccgacg ccgcgagctt ccggttgagc gcgccgatga cgcgctcggc
       61 gtacccggcg gcaatccccg ccgtgttcga gttcacgctg tccacgatga tctgcacctc
      121 ggcggcctgc cccttcgcgc gcgtccgggc gaagtcgtgc ggcacgatca gccccgcgtc
      181 cagttcgccc gcgctgatgg cgcgcccgag ttccgcgccc gaggcgtact ggcccgcgac
      241 gatgaacgag cggctctcga cgaaggccga caccagagtg cggctctccg ccgtgcggct
      301 ctcgtccacc acgccgagcc gcaagtcctt cacctccggg ttcatcgcga agcccaagag
      361 cacgaggttc accgtcggcg gcacgacgag catcatgacg agcttgcggt tgcgcttgat
      421 ctgccgcagc tccttcatga agagcgacca gaagcggctg tcgatgatct tcagcaacgg
      481 tttcatcacg cctccacctg catgctgcgc atgcgcttcc acgcggacag aaagaagaac
      541 gcgccgagca gcccgagcgc tacgggagcg ttccacaccg ccggccaccc gccgccgcgc
      601 acgaagacat cgcgcgagac ctcgatgtag taacgcgcgg ggatgaggta ggaaatccag
      661 cgcacccctt cggggatgtt cgagatcggg aagatgaaac cggccagcag gtacgacagc
      721 aggaagcccg tgttctgaat cgcctgaatc gccgccgcgc ggtcggggat gcgcgcgccg
      781 atcatcgcgc cgaacgcgac gacgcagaag agatagagca gcgagctgac gacgaacggc
      841 gtcgggtcgc ccttgaaccc gaggccgaag aggaacatcg cgaagacgag cgtcagcacc
      901 cactcggcgg cggcgatgat gccgaacgcg agaatcttgc cgagcaggta ttcgtgcgcc
      961 gagatgctcg acacgtagac ttgcagaatc gtcttctgct cgccctcgcg cgacatggcc
     1021 agcgccgcca gcagcggcgg gaagagcgct agcccaacgg cgaagatgcc cggcccgatg
     1081 tacttgtccg agtcgcgtcc ggggtggtac cagaagcgca ccgccgggac gattgatccc
     1141 cgctcgcgcg cgggcgcgac ctcggcattg aacgcctgcg tgatggccgc cgcgtgcccg
     1201 cgcatgatgt tggcggtgtt ggcgtccgtg ccgtcgagca gccactgcac ctcggcgccg
     1261 aggccgcgcc gcaggtcgcg gtcgaagccc tcggggatga cgaccgccgc gtgcgccgtc
     1321 tcgtcttcga gcgcgttctc ggggcgctcg ccggcttcga gcggcacgac gcggaacgtc
     1381 agcgacgcgc gcagggcctc cgcgtagcgg cgcgacagcc gatggcgatc tggttggcga
     1441 tctgcccgag cagctcggcg tccgcctcgg tgaaggcggc ctcgcggagg ctgccgacgt
     1501 tgatcgtgcc gagcacgcgg tcgtgcgaga cgagcgggac ggagcagccc gagcgcagcc
     1561 ccgcttcgta ggcgagacgc atcaccggcg agtggaactc tgcgaggtcg atgcgctcgc
     1621 gccgcacggt cgcgcgcgtc ttgatggcga gtccgggcgg cgtgccttcc atcggcagca
     1681 ccgacccttc ggcgaggacg ccgcccggcg cgggcgcatc gagcgcgtgc acgcgcagtt
     1741 gccccgtcgc ctcgtcgtac agcaacacgc tggcgaggtc gtggtggacg ttccggctga
     1801 tgtgcgtgga gatgttgagc agcaggtcgc gcagactgag gtgcgagacg acggcgttgt
     1861 ttacgtcgag cagcagctcg gcgcgcgctc gctcgcgcgt cgcgctctcg aagttgagcg
     1921 cgttctcgac ggcgatggcg acctgtttga cgacctgctc aaggaacgca aggctctcgg
     1981 cgtcgcaccc gtggcgctcc tgacagccga agcccatcgt gccgaggcgg cgcccggcgg
     2041 acgtgagcgg gaacgagtag aaggagtgga tgccgtagcg gcggaagagg tcggtcgtga
     2101 acgggaagcg tcgttcctca tccacgtcgt ggacgatcag gggctgctgc gtctgccaca
     2161 cccagccggc gccggtgcct tcgacgggca cctcgtagcc cgccggcaga ccgtccgggt
     2221 cgcgccgctc catcacgtgc aggcgcatca cgccgcgcgc ctcgtcgtgg agcaggacgt
     2281 tgatgtagtc gaagctgacg agggggcgga gctgcgcggc gaggtcgtgg aaaagctccg
     2341 cgaggttgcg gtgcgagatg atggattcgg tgacgccgag caggctctcg aactgctcgg
     2401 ccatcgaacc cggcgcgggg gacttcgctg gatcgctcat gtctgactgc tcgctccgga
     2461 gggggttcgt ctacggctga cccggcccgc ccctgctcgg tgtgcgttga accggtggaa
     2521 gatgaaacga ggtgattaag aatgaagccg gcgaggtgat gaatatggcg accgccgtcc
     2581 gcgaatatat tttaaccgcg ccgaaatgga aagcacatgc ggctccgccg cctgatgtgc
     2641 ggtgaggctt agcctcaccg ttgaggtgct tctctgttta gctgaggctg cgcctcagcg
     2701 cgggggcata gccctggaat aattcgagtg cgaccgcgac cggaagggca cagcccttcc
     2761 gcacatcagg cggcggagcc gccgaaggtc tcgactttcg ccggagttct actcgacccg
     2821 gaaccagtcg aagtccgcgt agccgacctc gcgcgccgtg cccgtgcgca ccgcgaagag
     2881 gccgagcgtc gagccgatcc agcggccctg ccgcgccgtg aaaggctcgc cgaccgtcgt
     2941 gaacgtcttc ccgtcggcgc tgtagctgaa cgtgcagacc gcgccctccg cgaccttcac
     3001 gcgcaggtaa aaggtgtcgc ccttcaccgg cagggcggcg ccctccctct ccggcccgcc
     3061 cttgtccgcg tccttgcaga cggtctgcga gacgaagagc tgctcgcctt tcttcttcag
     3121 cgagaggtac gcgtagtcga ggcccgtcac gatcagcccg gccttctcct cgtcgtggcg
     3181 cgcggtgaag gtgactttcg tcgtcgcggt gaaggcgggc gcggggaact tctgcgcgag
     3241 gatgttcggc acgtcccaga aatttttgaa gccctcgggc agcggcacgt tcagcagccg
     3301 caggtagccg tggcgcccgc ccagaaacat ccagttcgtc tgcgggttgg cgaaccactg
     3361 ccactgcaag ccgagcctcg tcgagtcgaa ctcgtccgac tcgggcggcg tctctgtcgg
     3421 gtacgacttt ccgacgttcg gctttttgaa cttcagcacc ggctcgcccg tcccgtcgcc
     3481 gtccgcgtcc gcgcccatca ccggccagtc gttcacccac cgcacgggct gtaggtggac
     3541 gatgcgcccg taaggccctt tgtcctgaaa gtggaggaac cagctctcgc ccgtctgcgt
     3601 ctcgacccac gcgccctggt gcggcccgtt gatcttggtc ttgccctgcg ccagcacgat
     3661 tctttcttcg taagggccgt agacgttcct cgaacgcagg acgagctgcc acccctgctc
     3721 gacgccgccc gccggggcga agatgtagta aaagccgttg cgcttgtgga acttcggccc
     3781 ctcgaccgtc gggtgcttct cgtggccgtc gaagacgatg accgggtcgt ccagcagctt
     3841 cgtcccgtcg gcgctcatgc ggctcacgac gaggatgctc ttgatgcccg aacggctcgc
     3901 tgcggtcgcg ctgacgaggt acgcgttgcc gtcctcgtcc cacaaagggc acgggtcgat
     3961 ccagcccttc gcttctttta tcagcagagg ctccgaccac ggccccgccg cgtctttcgc
     4021 cttgaccatg tagatgccgc ggtcggggtc tgggtagtag atgtagaact cgccgttgtg
     4081 atggcggatc gaaggcgccc acacgccgcc cccgtgctgc gtcttgcgga aaacgtcgta
     4141 gggcggctgc tcgcggaaga cgtggccgac aagccgccag ttgacgaggt ctttcgagtg
     4201 gaggatgggc aggcccggcg cggcgttgaa gctcgaagag gtcatgtagt aatcgtcgcc
     4261 gacgcgcacc gcgtccgggt cggagtagtc cgcgtgcagc accggatttt tataggtgcc
     4321 gtcgccgttg tccgcgaccc agactttgga gacggcgccg cccgcgcccg ccgccggagc
     4381 agtttgcgcc gcgctcgcgc aaggccagac gattaaagac aggacgagca agcagcttct
     4441 cttcgacaga ctttcgagtt tcattgtgtt cgcgtgtcga tgaatcgtga ataaggattc
     4501 ggggttcggt tccccgcggc ggcggcgttc gctcagccct cgaccctgtc tttgcgaccg
     4561 tagcccaact ccgccccgcc cgagatgacg tgatcgacgc cggtcgtgaa cgtcgcttcc
     4621 gccgcgatga acaggcagag gcggcctgcc tcttccgccg tccccatgcg cccgacgagc
     4681 tgcgcggcct cgcccgccgc gcgccaccgc gcggggtcgg gcgccgcgtc aatcgcctgc
     4741 tgccacagcg gcgtgtagat gttgcccggc gacaccgagt tgacccgcac gccgtgcgcc
     4801 gcctcgtcga tcgccaaagc cttcgtgaag gccgtcaccg cacccttcgt cgccacgtag
     4861 gtcgtgccgt ggagctgccc gatttgcgcg acgaggctcg acatgttgat gatgttgccg
     4921 cgggtgcggc gcaagtgcgg gagcgcgagc ttgcaggccg cgaagacgct gacgaggttc
     4981 agctccaaca attcgcggaa ctcttccagc gagaagtcgt cgatgggctt gtgcggcggg
     5041 tgccaccccg cgttgttgat gaggcagtcg atgcgccccg cgcggtgcac cgtctccgcg
     5101 acgaggtgtt cgaggtcggc ggctttcgtc acgtcgcacg cgacgaagtg cgccgcgccc
     5161 cccggcccgc gcgcgttcaa ctcggcggcg agcgccgcgc cctcgctctc gtcgcgcgac
     5221 gcgatgacga ccaacgcgcc cgcctccgcg aagacgcgcg cgcacccctc cccgatgccc
     5281 cggctcccgc cggtcacgat cacgacttta tccgtgtacc gcatcccgcg aaggataata
     5341 gctccgtgcg tctttatgca aagcgttccg cgccgccggc ccacccctga agcctgcatg
     5401 ccgcttcaat catcgtgccc ccccacggct cgtcggatcc acgtggggac tgtcaggtcg
     5461 ccccccttgc cccgcactct gtaagctcca cagctcttcg tccccgatcc actacctctc
     5521 gtcagcgcct gcggacggtt cgtcacattt cgcgaacctc gcgccgaaaa acctcgtcta
     5581 taccggcgta ggcacgtctc cccgaaccgg cattactctc gacagctaac cgcgaaaggc
     5641 tggaggtttc gacaatgaag ggcttagaca ttccgctctg ggcggtcggt atagcggctg
     5701 ccgcgtacgc gctctacgag ttcatgcggt tcgcgacggc gacagacccg gcgacgggct
     5761 tccaggacat gtgggcgggc gtcaaccacc tctacgtagg gctggccgcc gccgtggtgg
     5821 ccgccgtctg tatcgtctgg gccttcgtgc ggcggcccca cgagatggag gagattcacg
     5881 tcaccaagta agggcgtgag ggtgggtcgg gactgcccgc cgcgtctaca gcaacagccg
     5941 cgatcaacaa tactgcgagg ggaggtatct ctgcgcggcg ccggggaaaa cttgctgtca
     6001 tcaaaagtga tactgctggc gaagtcgaaa aactgtcggg aatagacgat ctgttcggat
     6061 cattgagccg aatttccgcg ataaccttat ccattactga cttcgccggc ggtatcaata
     6121 acgcgggtga accccaacat ggtagcggcg gtgaagaagc atgaacccgg agcgcatcgg
     6181 gaaggcggac gaggtcttcc gggcggcgct cgacccggcg tccgggccga gcgccgccta
     6241 cctcgtcgag gcctgcgcgg gcgacgagga gctgcggcgc gaggtcgaat ccctgctctc
     6301 gtctcgttgg ggcctttgcc gcggtcgcct ccctgtcgca tcttcaccct ctcggtaggt
     6361 attaagttac gcctcggcgc cggacgcgga cgaagctccg gcgcgcccgg ccccgcctca
     6421 ggctttgccg cgcttccagc gcccgcacct cgtgcccggc tccttcctcg cgtccttgtc
     6481 cttctccttc tgctcgcgct ccgcctgctt gcggtcgcgc tcggcctgct cctgcgcctg
     6541 ccggtcttgc tgctgcgtcg tcgcgccctg cggcgtctgc gcgcgtagtg cgcctgccga
     6601 cagcgcgcag aggacgagtg tcgtactgat gagtttgaac atggcactcc tctccccggg
     6661 ggacggtgtt ggcttgggaa ctcggcgggg gaatggtgta acgatattgc ttcgcggggc
     6721 gcccgtcaac agggcgtggc atcctgacca aagccattcg ccctgccgcg c
//
LOCUS       DBNGOH010001045         7775 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_891005, whole genome shotgun sequence.
ACCESSION   DBNGOH010001045 DBNGOH010000000
VERSION     DBNGOH010001045.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7775)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7775)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7775
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_891005"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(108..1649)
                     /locus_tag="AC159P_20300"
     CDS             complement(108..1649)
                     /locus_tag="AC159P_20300"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3697841.1"
                     /translation="MKRATGIALAFVFVFMSAAVCAPAIAQKGNSKSGTLSEKSYARA
                     REVLEAGLRALGGVEAIRAAEDVSVKMSGHGLARNQSVSIEGAYDRARHEETLYIDLR
                     NRRYVVETRDELPGGFVFGGRTVVNGNQGFFVNPRDRTVTPVNMANFNNIGIIRRVPH
                     VMLLAAYETGAATLRYLGEDTFDGRAHHVITAAATNGTQWSLYFDAKTNLLSKYELVV
                     SDPLAGDAVREWVFPGYRAVGSLKVPTGRITRQAGEVVEEVTYDDVQLNTRPAESAFA
                     KPEGHEELPQPTPAATRETKLAEGVYLFESGANSLVVEFADHVMVVEPYTGGRGAKAV
                     ITKAKEMFPAKPVRYVVVTHFHDDHSGGLRPYVAEGTSIVTTAANRQFFERMAAGTFT
                     VARDDQTAARKKPAFEFVEKGRRVFTDGKQTVEVIDIGPGPHAREMLVVYLPKEKLVF
                     QGDLVNLPNRGRWMPTTVNDSTVHFLDSLSRLNLDVQRIASVHGPATTIEDLRTAVDK
                     KRAAK"
     gene            complement(1810..4692)
                     /gene="uvrA"
                     /locus_tag="AC159P_20305"
     CDS             complement(1810..4692)
                     /gene="uvrA"
                     /locus_tag="AC159P_20305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HIR3697842.1"
                     /translation="MTEEAIQVRGARVNNLKNVTFSIPIEKLTVVTGVSGSGKSSLAF
                     DTVYAEGQRRYVESLSAYARQFLERMDKPDVDEVRGIPPAIAIRQKNATRNPRSTVGT
                     QTEVYDYLRLLFARVGTTVCRVCGREVRRDSPESAAQETLDALPEGTRFYVLFPADAG
                     MRVEANGDAKKNGSRKRAAKAKDANQTYDARQVTAHVMSLMQRGFTRLYADGDIVELR
                     SPDDYARPDFEGVHVLVDRLVARADVRERLVDSFETCFVEGHGQAVIRTADAEPRALR
                     FSERFECKYDGTLYAVPEPRLFSFNSPFGACPTCQGFGNTIGLDLDLVIPNPSLSLAE
                     GAIEPWTKPQHEWARTELRRFARQEIIPLDVPFAELTRQQQRAVVEGKGKWEGVRGFF
                     EWLETKKYKLHVRVFLSKYRGYTLCPDCGGGRLRQEARDVRVGGRTLPEVCSLSIKDA
                     TRFFEELELTTEQTAIADKVLLEIRRRLKFLVDVGLDYLTLDRLASTLSGGEAQRIQL
                     ATNLGSSLVGALYVLDEPSIGLHPRDSERLIHLLHNLRDIGNTVLVVEHDEEMMRAAD
                     HILDIGPAAGELGGKVVYEGDFAGLLKEGATLTGKYLRGEQEIKEPKQRRAPKKKRLS
                     VRGASEHNLKDVDIDIPLEVFVCVTGVSGSGKSTLVHDVVYAGLKKQRGEWQGHVGTF
                     RELAGAQHVDEVILVDQSPIGRTPRSNPVTYVKVYDAVREVFSQTRDAQSRGFDPSHF
                     SFNVPGGRCDVCEGDGTVTVEMQFLADVELVCEECKGTRFKPQLLDVRYRGKNIHDVL
                     NMTVREAMTFFRDTSKIANRLRVLDDVGLGYLRLGQSATTLSGGEAQRVKLAAHLSKR
                     AGARTLYIFDEPTTGLHFDDINKLLAAFRTLIEAGGSLLVIEHNLDVIKTADHVIDLG
                     PEGGDFGGQLVATGTPEEIMNAPHSHTGRFLKQHLARSNGHHARAS"
     gene            complement(5014..6246)
                     /locus_tag="AC159P_20310"
     CDS             complement(5014..6246)
                     /locus_tag="AC159P_20310"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HIR3697843.1"
                     /translation="MNRINRRLFALQGGAALSAALAPRSFGNVAPEENGRRALTTRML
                     SRNFLAGLPRLMELAYVPGLSIAVVEGGRVTWARGFGVKKAETKDAVDAETVFPAASL
                     SKPVFAYGVLRLREEGLLDLDRPLADYLPGAYALEDARAKLITARHVLSHTSGFQNWR
                     FAKDDKLQIAFDPGARFQYSGEGYFYLQRVVEQLTGQSVTRFMRERVLGPLGMTKSSY
                     AWLPEYEKLTVWGHRNRGQAGEGFAARAGRKMEEVAARLNKPVEEWKYEDVVRAMPEV
                     DANWNGHPVGLLPNVAGTLNTTAPEFARFMVRVMRRAARDAHDLKESSRREMLRPQVR
                     INGALAWGLGWGLESAAGSDYFWHWGDNGTFHCFAIGDPSQRSGLVVFTNSNFGPKLY
                     QRIVNHATGHDHPAFLWI"
     gene            complement(6480..6833)
                     /locus_tag="AC159P_20315"
     CDS             complement(6480..6833)
                     /locus_tag="AC159P_20315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697844.1"
                     /translation="MSTTLERPPGWEAFDALTGGLSSSVEPPLTTEGAARTLGSLPVG
                     ARLVVRCRKDWRAATVAAFEREAARVVLNVASPSGHTYRLRRPPDALLSFDGAIPLLG
                     EGVWRAGLARYDVRW"
     gene            complement(6845..7624)
                     /locus_tag="AC159P_20320"
     CDS             complement(6845..7624)
                     /locus_tag="AC159P_20320"
                     /inference="COORDINATES: protein motif:HMM:NF024790.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HIR3697845.1"
                     /translation="MRFKIAHDARPQVATLLWATALTVALWFIPFAEVLTYPFRIFVT
                     FIHEGGHALAALVTGNSVHSLNVSMDGSGEVYSSAGGVLSQMFVSSSGYLGAMAFGVV
                     LLLLIRRTVAARYVLAGTAALILALTIFFGFLAPVWNLSLPGLFTLVAGVALPVGLFA
                     VARYAGAKVAAFLVSFLAVQCVLNALFDLKSVLLLSTFTNAQTDAANMARATGVPEIF
                     WAVFWIGVAFLLLSLALRAYSVSKSKPAQPDLPFEDSPLEV"
BASE COUNT         1274 a         2533 c         2659 g         1309 t
ORIGIN      
        1 tacagcgcag gcgacagacg gtcaatgacg attgatgaca gcactgctgc catcaatcgt
       61 cgctgctgct tcaatccgca atccgaaatc cgcattccgc aatcgaacta cttcgccgcc
      121 cgcttcttat cgaccgcggt gcgcaggtct tcgatggtgg tggcggggcc gtggacggag
      181 gcgatgcgct gcacgtcgag gttgaggcgg ctcagggagt cgaggaagtg gacggtcgaa
      241 tcgttgacgg tcgtgggcat ccacctgccg cggttgggga ggttgacgag gtcgccctgg
      301 aagacgagct tctccttcgg caggtagacg acgagcatct cgcgcgcgtg cgggccgggg
      361 ccgatgtcga tgacctcgac cgtctgcttg ccgtcggtga agacgcggcg gcctttctcg
      421 acgaactcga aggcgggctt cttccgcgcc gccgtctggt cgtcgcgcgc gacggtgaag
      481 gtgcccgccg ccatgcgctc gaagaactgc cggttggccg cggtcgtcac gatcgaagtg
      541 ccctcggcga cgtacgggcg caggccgccc gagtggtcgt cgtggaagtg cgtgacgacg
      601 acgtacctga cgggcttggc cgggaacatc tccttcgcct tcgtgatgac ggccttcgcg
      661 ccgcgcccgc ccgtgtaagg ctcgacgacc atgacatgat cggcgaactc gacgacgagc
      721 gagttggccc cgctctcgaa gaggtagacg ccttcggcga gcttcgtctc gcgcgttgcg
      781 gcaggcgtgg gctgcggcag ctcttcgtgc ccttcgggct tggcgaaggc gctctccgcc
      841 gggcgcgtgt tgagctgcac gtcgtcgtac gtgacctctt cgaccacctc gcccgcctgc
      901 cgcgtgatgc gtcccgtggg caccttcaag ctgccgaccg cgcggtagcc cggaaagacc
      961 cactcgcgca cggcgtcgcc cgcgagcggg tcgctgacga ccagctcgta cttcgacagc
     1021 aggttcgtct tcgcgtcgaa gtagagcgac cactgcgtgc cgttcgtggc ggcggcggtg
     1081 atgacgtggt gcgcgcgccc gtcgaacgtg tcctcgccga ggtagcgcag ggtggccgcg
     1141 cccgtctcgt acgccgcgag cagcatcacg tgcggcacgc ggcggatgat gccgatgttg
     1201 ttgaagttcg ccatgttgac cggcgtcacg gtgcggtcgc gcgggttgac gaagaaaccc
     1261 tgattgccgt tgacgacggt gcgcccgccg aagacgaagc cgcccggcag ctcgtcccgc
     1321 gtctcgacga cgtagcggcg gttgcgcagg tcgatgtaca aagtctcctc gtgacgcgcg
     1381 cggtcgtacg cgccctcgat gctgacgctc tggttgcgcg cgaggccgtg cccggacatc
     1441 ttcaccgaca cgtcctccgc cgcgcggatc gcctcgacgc cgcccagcgc gcgcaggccc
     1501 gcctccaaca cctcgcgcgc acgcgcgtac gacttctcgg acagcgtccc gcttttcgag
     1561 ttgcccttct gcgcgatggc cggcgcgcac accgccgccg acatgaacac gaacacgaac
     1621 gcgagcgcga ttcctgttgc tctcttcacg gggaaagtcc tccttaactt cggttgtgtg
     1681 gataaacggc tgcgagagaa tgcgacccgc ttgaaaggct gctgacgagt agatacgcgc
     1741 gccgcccaag cgggttgcgc cgcgcccgta aaagaattgt aaaaaggtcg tgcggggcgg
     1801 agcgggaggt taactcgcgc gcgcgtggtg gccgttggag cgggcgaggt gctgtttgag
     1861 gaagcggccc gtgtgggagt ggggcgcgtt catgatctct tcgggcgtgc cggtggcgac
     1921 gagctggccg ccgaagtcgc cgccttcggg gccgaggtcg atgacgtggt cggcggtttt
     1981 aattacgtcg aggttgtgtt cgatgacgag gagcgagccg cccgcttcaa tcaaggtgcg
     2041 gaaagcggcg agcagcttgt tgatgtcgtc gaagtgcaga ccggtggtcg gctcgtcgaa
     2101 gatgtagagg gtgcgtgcgc cggcgcgttt ggagaggtgc gcggcgagct tgacgcgctg
     2161 ggcttcgccg cccgagagcg tggtggcgga ctggccgagg cggaggtagc ctaatccgac
     2221 atcatccaga acgcggaggc ggttggcgat tttcgaggtg tcgcggaaga aggtcatcgc
     2281 ctcgcgcacg gtcatgttga gcacgtcgtg gatgttcttg ccgcggtagc gcacgtcgag
     2341 aagctgcggc ttgaagcgcg tgcccttgca ctcttcgcag accagctcca cgtcggcgag
     2401 gaactgcatc tcgacggtga cggtgccgtc gccctcgcac acgtcgcagc gcccgcccgg
     2461 cacgttgaag gagaaatgcg aggggtcgaa gccgcggctc tgcgcgtcgc gcgtctgcga
     2521 gaagacttcg cgcaccgcgt cgtagacctt gacgtaggtg acggggttcg agcgcggcgt
     2581 gcgaccgatg ggcgactgat cgacgaggat cacctcgtcc acgtgctgcg cgcccgcgag
     2641 ttcgcggaag gtgccgacgt ggccctgcca ctcgccgcgc tgttttttga ggccggcgta
     2701 gacgacatcg tggacgagcg ttgacttgcc ggagccggag acgcccgtca cgcagacgaa
     2761 gacctccagc gggatgtcga tgtccacgtc cttcaggttg tgctcgctgg cgccgcgcac
     2821 ggagaggcgc ttcttcttcg gcgcgcgccg ctgcttcggc tccttgatct cctgctcgcc
     2881 gcgcaggtac ttgcccgtca gcgtcgcgcc ctccttgagc agccccgcga agtcgccctc
     2941 gtaaaccacc ttgccgccca gctcgcccgc cgccgggccg atgtcgagga tgtggtcggc
     3001 ggcgcgcatc atctcctcgt cgtgctcgac gacgagcacc gtgttgccga tgtcgcgcag
     3061 gttgtgcagc aggtggatga ggcgctcgct gtcgcgcggg tggaggccga tgctcggctc
     3121 gtccagcacg tagagcgcgc cgacgagcga cgatccgagg ttggtggcga gctggatgcg
     3181 ctgcgcctcg ccgcccgaga gcgtcgaggc gaggcggtcg agcgtcaaat aatccagccc
     3241 gacatcgacg aggaacttca gccgccgacg aatctcaagc agcaccttgt cggcgatggc
     3301 cgtctgctcg gtcgtcaatt cgagttcttc gaagaagcgc gtcgcgtctt tgatcgagag
     3361 cgagcagacc tcgggcaggg tgcggccccc gacacgcacg tcgcgcgcct cctgtcgaag
     3421 tcttccgccg ccgcagtccg ggcagagcgt gtagccgcgg tacttcgaga ggaagacgcg
     3481 gacgtgcagc ttgtacttct tcgtctccag ccactcgaag aagccgcgca cgccctccca
     3541 cttgcccttg ccctcgacga cggcgcgctg ctgctggcgc gtcagctccg cgaagggcac
     3601 gtccagcggg atgatttcct gacgcgcgaa gcggcgcagc tccgtccgcg cccactcgtg
     3661 ctgaggcttc gtccacggct cgatggcgcc ctcggcgagc gagaggctcg ggttcgggat
     3721 gaccagatcg aggtcgaggc cgatggtgtt gccgaagccc tgacaggtcg ggcacgcgcc
     3781 gaaggggctg ttgaagctga agaggcgcgg ctcggggacg gcgtagaggg tgccgtcgta
     3841 tttgcactcg aagcgctcgg agaagcgcag ggcgcgcggc tcggcgtcgg cggtccggat
     3901 gacggcctgc ccgtggcctt cgacgaagca ggtctcgaag gaatcgacga gacgttcgcg
     3961 cacgtcggcg cgcgcgacca gccggtcaac gagcacgtgg acgccctcga agtcggggcg
     4021 cgcgtagtcg tcgggcgagc gcaactcgac aatgtcaccg tccgcgtaga gccgcgtgaa
     4081 gccgcgctgc atgaggctca tcacgtgcgc cgtgacctgc cgcgcgtcgt aggtctgatt
     4141 cgcgtccttc gccttcgcgg cgcgcttgcg gctgccgttc ttcttggcgt cgccgttggc
     4201 ctcgacgcgc atccccgcat ccgccgggaa gaggacgtag aagcgcgtgc cctctgggag
     4261 cgcgtcgagc gtctcctgtg cggctgattc cggcgagtcg cgccgcacct cgcgcccgca
     4321 gacgcggcag accgtggtgc ccacgcgcgc gaacagcaga cgcaggtagt cgtagacctc
     4381 tgtctgcgtg ccgacggtcg agcgcgggtt gcgcgtcgcg ttcttctggc ggatggcgat
     4441 ggccgggggg atgccgcgca cctcgtccac gtccggcttg tccatccgtt cgaggaactg
     4501 ccgcgcgtag gccgacaggg actcgacgta gcgccgctgc ccctccgcgt acaccgtgtc
     4561 gaacgcgagc gaagacttgc ccgacccgct cacgcccgtg acgaccgtca gtttttcgat
     4621 ggggatggag aacgtgacgt tcttcagatt gttgacgcgc gccccgcgca cttggattgc
     4681 ttcttcggtc atggctcgaa aacacgcgga ggggtgcaca gtcagctccc cggcccctcc
     4741 gcgaaccacc catgatacct tgaatcagcg cggagacgaa acgcggtcgc gggttttatg
     4801 tttaagggcg ctcgttggtt gagtttgtcg cggggccgga ttcaacgcgc aggacacaga
     4861 ggcgcagagg ccgcagagga aaccaatcac ggatttgaga tttcggattc aaatcccggc
     4921 tttcctctcg gcgaactctg cggccctccg cgacctccgc gttaaacccg acccccgtta
     4981 agctcaaccg ccgggcgttt ttatactctc cccttaaatc cacaggaacg cggggtggtc
     5041 gtggccggtg gcgtggttga cgatgcgctg gtagagcttg gggccgaagt tgctgttggt
     5101 gaagacgacg aggccgctcc tctgcgaggg gtcgccgatg gcgaagcagt ggaaggtgcc
     5161 gttgtcgccc cagtgccaga agtagtcgct tcccgcggcg ctctcaaggc cccagccgag
     5221 gccccacgcg agcgcgccgt tgatgcgcac ctgcggcctc agcatctcgc gccgcgaaga
     5281 ctctttgagg tcgtgcgcgt cgcgcgccgc gcgccgcatg acgcgcacca tgaagcgtgc
     5341 gaactcgggc gcggtggtgt tgagcgtgcc cgcgacgttc ggcagcagcc cgacggggtg
     5401 gccgttccag ttcgcgtcca cctccggcat cgcgcgcacc acgtcttcgt acttccactc
     5461 ctcgacgggc ttgttcaggc gcgcggccac ctcctccatc ttccgaccgg cgcgcgccgc
     5521 gaaaccctcg ccggcctgcc cgcggttgcg gtgcccccac acggtcagct tctcgtactc
     5581 cggcagccac gcgtagctgc tcttcgtcat gccgagcggg ccgagcacgc gctcgcgcat
     5641 gaagcgcgta acggactggc cggtgagctg ttcgacgacg cgctggaggt agaagtagcc
     5701 ctcgcccgaa tactggaaac gcgcgccggg gtcgaaggcg atttgcagct tgtcgtcttt
     5761 ggcgaagcgc cagttctgga agcccgacgt gtgcgacaga acgtggcgcg cggtgatgag
     5821 cttcgcgcgc gcgtcttcga gcgcgtaggc gccgggcaga tagtccgcga gcgggcggtc
     5881 gaggtcgagc agcccctctt cgcgcagccg caggacgccg taggcgaaga ccggcttcga
     5941 cagcgacgcc gcggggaaga ccgtctccgc gtccaccgcg tccttcgtct cggccttctt
     6001 cacgccgaag ccgcgcgccc aagtcaccct gccgccctcg acgaccgcga tggacaggcc
     6061 gggcacgtac gccagctcca tcagccgcgg cagccccgcc agaaagtttc tcgacagcat
     6121 ccttgtcgtc aaggcccttc ggccattctc ttcgggcgcg acgttgccga acgaacgcgg
     6181 cgcgagcgcg gctgagaggg cggcgccgcc ctgtagcgcg aagagtcgtc tgttgattcg
     6241 attcatcttt cccccatgac ggacgagagt ggaaggcgga cgcgtccgac atgatagagc
     6301 ttttcgcgat tattttggac gaccatgtga cgggggcgag aattacggtg acgagatggc
     6361 cggaggcagt gagcagtgag cggtgagcgg tgagcagtca gaagaaagtt cggccttcaa
     6421 cggccacagg cactcgataa gaactgctta ccgctcaccg ctcaccgctc actgaccttt
     6481 taccagcgca cgtcgtagcg cgcgaggccg gcgcgccaga cgccttcgcc gagcagcggg
     6541 atcgcgccgt cgaaagagag gagagcgtcg ggcgggcgcc gcaggcggta ggtgtggccg
     6601 gagggcgagg cgacgttgag gacgacgcgg gccgcttccc gctcgaaggc ggcgaccgtc
     6661 gccgcgcgcc agtccttgcg gcagcgcacg acgagccgcg cgccgacggg cagcgagccg
     6721 agcgtccgcg ccgcgccttc ggtcgtcagc ggcggttcga cggacgaaga gaggccgccg
     6781 gtcaaagcgt cgaacgcttc ccatcccggc ggcctctcaa gggtcgtgct cacggcttga
     6841 gttttcagac ttcgagcggc gagtcttcga acggcaggtc gggctgcgcg ggcttgctct
     6901 tgctcaccga gtaggcgcgc agcgcgagtg acagcagcag gaacgcgacg ccgatccaga
     6961 agacggccca gaagatttcc ggcacgcccg tcgcgcgcgc catgttggcc gcgtccgtct
     7021 gcgcgttcgt gaaggtcgag agcagcagca ccgacttcag atcgaagagc gcgttcagca
     7081 cgcactgcac ggcgaggaag ctgacgagga acgccgccac cttcgcgccc gcgtagcgcg
     7141 ccacggcgaa cagcccgacc ggcaaagcca cgcccgcgac cagcgtgaac agccccggca
     7201 ggctcaggtt ccacaccggc gcgaggaacc cgaagaagat cgtcagcgcc agaatcagcg
     7261 ccgccgtgcc ggcgagcacg tagcgcgcgg ccaccgtccg ccggatcagc agcagcagca
     7321 ccacgccgaa cgccatcgcg cccaaatagc ccgaactcga cacgaacatc tgcgacagca
     7381 cgccgcccgc agacgagtag acctcgccgc tcccgtccat cgacacgttc aggctgtgca
     7441 cggagttacc cgtcacgagc gccgccagcg cgtgcccgcc ctcgtggatg aacgtgacga
     7501 agatgcggaa cgggtaggtc agcacctctg cgaacgggat gaaccacagg gcgacggtca
     7561 gcgccgtcgc ccacagcagc gtcgcgacct gcgggcgcgc gtcgtgtgcg atcttgaatc
     7621 tcatctctgg aaatctccgc tctgaaaaaa acttccgacc ccacgctgtc gtgtacgaca
     7681 ttctacgact aaggttcgca gaagaaaagg agtaaaagcc gtgaaccgtg aaccgtcaac
     7741 cgtgacaaga aaaaacaggt tcttacttgt cacgg
//
LOCUS       DBNGOH010001046         6175 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1076898, whole genome shotgun sequence.
ACCESSION   DBNGOH010001046 DBNGOH010000000
VERSION     DBNGOH010001046.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6175)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6175)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6175
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1076898"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..664)
                     /locus_tag="AC159P_20325"
     CDS             complement(<1..664)
                     /locus_tag="AC159P_20325"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013087.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3697846.1"
                     /translation="MSSTNQRGDYEKQHRGTEAAYEAYFAGMDASMQQKVALTTAHFP
                     VRGRIADMGSGSGRGTYDLACLDGGLELVGVDINPVSVERAGREFRRPNLSYVVGDIS
                     EMVFPAESLDGILDSSVLHHVTSFNDFDVNRVLATLDRQVAQLRTGGVIIIRDFVIPE
                     GPERVHLDLPETDGSDAGGVGELSSAALFELFARDWRSSVNRDGPMPFARLKSPRAGF
                     ARY"
     gene            complement(692..2449)
                     /locus_tag="AC159P_20330"
     CDS             complement(692..2449)
                     /locus_tag="AC159P_20330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697847.1"
                     /translation="MALKAVFPGLGALLKGDAAAGRYFVQGGGEVRLGQSDFKAQGGE
                     GAIYVKGPNAYKIYTDPRRAIPPAKILELSALTLPNIIRPLGVLLDTKSKPVGYSMRA
                     VGRSYALCQLFPKAFRRRHNLTPESAFRLVRRLQEGVGHVHAGGVLVVDLNELNFLVA
                     EDFSEVYFIDVDSYQTASYPATVLMESVRDRHARSFTTGSDWFSFAVVSFQLFVGIHP
                     YKGNYPPLQGLPDKEKKLDARMRANVSVLRPEVSVPASCLPFSVIPPAYLDWYRAVFE
                     DGQRLPPPDDARPAVTLAAQGPSRGVGGAHFEITEAGEFDDEIVWHDGLITITRRSVY
                     FRGEKFPKPHADIKVAVTPRQGHLVAAYMDGGRPRLRDLTGSADLPLGVEGEEVMLCN
                     GQLYVKQRESILAVEFVELRGDILPAVRPVANVMMKSTRLFDGLAIQNLLGAYYASIL
                     PAPGVCHQVRLPEIDGYQILDARLYRNVLMVVGARGGQYDKLVFRFADDFGDYDSRLM
                     PDVSSTEIDFTVLDSGVVLHVNDEGALEVFSRLKGSADVKIFQDASVGGDDRLFHTGR
                     QALIARGGKLYKFTMRRRP"
     gene            complement(2452..3312)
                     /locus_tag="AC159P_20335"
     CDS             complement(2452..3312)
                     /locus_tag="AC159P_20335"
                     /inference="COORDINATES: protein motif:HMM:NF025053.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HIR3697848.1"
                     /translation="MNADSAFRIGSSHAVCQDYSLAGAWPPSAHAESRGPRPYVILSD
                     GCSSSPDTDIGARLMVKAAERTLREHGGPPGRDLAEIHEEAARLALEWAELLGLRSQA
                     VDATLLTAHLDGEELAIGCSGDGVVCLQTREGALDVYSIAYPSGYPLYPSYTHQPERL
                     LALGDAGRACKEVRHYRSASVEDRLQPRGATAGGPPTEVFTVKAADYECAALFSDGIH
                     SFFRAGQTEAGGGVEPVPLDLVLRDLVSFKTTRGAFVGRRMKRFLKDCRRKGWQHADD
                     LAVGALHTGG"
     gene            complement(3390..3740)
                     /locus_tag="AC159P_20340"
     CDS             complement(3390..3740)
                     /locus_tag="AC159P_20340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018865117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RyR domain-containing protein"
                     /protein_id="HIR3697849.1"
                     /translation="MTIEQIARICHDANASLCHSVGDHSQTSWGEAPEWQRESAIKGV
                     QFHLANPDASASATHDIWLKEKAETGWVYGESKDAAAKTHPCIVPFEQLPPEQQAKDH
                     LFRGIVHALAGFVA"
     gene            complement(3893..4636)
                     /locus_tag="AC159P_20345"
     CDS             complement(3893..4636)
                     /locus_tag="AC159P_20345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HIR3697850.1"
                     /translation="MPILNDTSLDQVTLPNSHYGYSATRLEELGATEYTVVTVACDVS
                     GSTAAFVFDMEAAITRIVQACKSSPRADNLLLRLVAFDDALVELHGFKLLENCNLADY
                     GGVLRAGGSTALYDAAENAVSSTTNYGQQLLAGDFSANAILFVITDGMDNASRLPAGK
                     VKEALTKAVTSEALESMVSVLIGVNVQDPQAARFLRQFHADAGFTQYVELDKADAKTL
                     ARLAEFVSRSISAQSQALGTGGPSQPLVF"
     gene            complement(4655..5458)
                     /locus_tag="AC159P_20350"
     CDS             complement(4655..5458)
                     /locus_tag="AC159P_20350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HIR3697851.1"
                     /translation="MSKRIELLIIDPQVDFCDPQRGALYVPGAEQDMGRLAAMVRRLR
                     NKLDDIHVTLDSHHFIHIAHPIFWKDSKGANPPVFTRISRAEVEEGVWSPTVPGMYRR
                     ALDYVRRLEQNGRYELTVWPPHCLIGSPGHAVFPPLFAALTEWESRFAFVDYVTKGSN
                     ILTEHYSAVQADVPDPSDASTQINTRLIQTLENADLVAIAGEARTHCLANTVRDIANS
                     FGDDSFVSKLVLLTDASSDIPGFEAHAQNFMSEMVGRGMQLSTTTAFLA"
     gene            complement(5522..>6175)
                     /locus_tag="AC159P_20355"
     CDS             complement(5522..>6175)
                     /locus_tag="AC159P_20355"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002633959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR3697852.1"
                     /translation="VEETLKVMLVERDLEPFAGMWAIPGGFVRRGETLEAAATRELQE
                     ETGIAQVFLEQLYTFGDPARDPRGWVVSVAYYALVSPDKHTIHAATDARQARWFPVGS
                     LPRLAFDHAEILKTALDRVRGKLTYAPIGFELLPRKFTIKQLQKLYEIVLGTQLDNRN
                     FRKKIFGMDVLRELDEMQKGVPHRAARLYKFDERKYRQLVKRGLSFGLWSATTTGAV"
BASE COUNT         1040 a         1973 c         2075 g         1087 t
ORIGIN      
        1 cgtaacgggc gaagcccgcg cgtggggatt tcaggcgggc gaacggcatc ggcccgtcgc
       61 ggttaacgct gctgcgccag tcgcgtgcga acagctcgaa cagggccgcg cttgataact
      121 cgccgacgcc tcccgcgtcg gagccgtccg tctccggcag atcgaggtga acccgctccg
      181 gcccttcggg gatgacgaag tctctgatga tgatgacgcc gccggtcctg agctgcgcca
      241 cctgtctgtc gagggtggcg agcacgcggt tcacgtcgaa gtcgttgaag ctggtgacgt
      301 ggtgcagcac ggaggagtcg aggatgccgt cgagcgattc cgccgggaag accatctcgg
      361 agatgtcgcc caccacgtaa gataggttcg ggcgccggaa ctcgcggccc gcgcgctcca
      421 cagagaccgg gttgatgtcc acgccgacca gctccagccc cccgtcgaga caggccagat
      481 cataagtgcc gcggcccgac cccgacccca tgtcggcgat gcgcccgcgg acggggaagt
      541 gcgccgtggt gagcgccacc ttctgctgca tcgaggcgtc catcccggcg aagtacgcct
      601 cgtaagcggc ctcggtgccg cggtgctgtt tctcgtagtc gccgcgctgg ttcgtggaac
      661 tcatctgtgg gtgttgtcga gcgcccgtgt gtcagggccg ccgccgcatc gtgaacttgt
      721 acagcttgcc gccgcgcgcg atgagcgcct gcctcccggt gtggaaaagt ctgtcgtcac
      781 cgccgacgga cgcgtcctga aagattttaa cgtccgcgga acctttgagt cttgaaaaga
      841 cttccagagc gccttcgtcg ttcacgtgca acacgactcc gctgtcgagc accgtgaagt
      901 cgatctcggt tgacgaaacg tccggcatca accgcgagtc gtagtcgccg aaatcgtccg
      961 cgaagcgaaa gacgagcttg tcgtactgcc cgccacgtgc cccgaccacc atcagcacgt
     1021 tgcggtagag ccgtgcgtcg agaatctggt agccgtcgat ttcgggcagg cgaacctgat
     1081 ggcacacgcc gggtgcgggg aggatcgaag cgtagtatgc ccccagtaga ttctgaattg
     1141 ccaacccgtc gaagagccgt gtggacttca tcataacatt ggccacgggc cggacggcgg
     1201 gcaaaatatc gccccgcagc tcgacgaact cgacggccag aatgctctcc cgctgcttga
     1261 cgtagagctg gccgttgcac agcatgacct cctcgccctc gactcccagc ggcaggtctg
     1321 cggagccggt caggtcgcgc aggcgcggtc tgcccccgtc catgtaggcg gccaccagat
     1381 gcccctgccg cggcgtcacg gccaccttga tgtcggcgtg cggcttcggg aacttctccc
     1441 cgcggaaata gacgctcctc cgtgtaatcg taatcagccc gtcgtgccag acgatctcgt
     1501 cgtcgaactc tcccgcttcc gtgatctcga aatgagcgcc gccgacgccg cgtgacggcc
     1561 cctgcgcggc gagcgtgacg gcgggccgcg cgtcgtcggg cggggggagc ctttgtccgt
     1621 cttcgaagac ggcccgatac cagtcgaggt aagcgggagg gatgacggag aacggcagac
     1681 aggacgcagg gacggagacc tcgggcctca gcacggagac gttggcgcgc atccgtgcgt
     1741 cgagtttttt ctccttgtcg ggcaatccct gaagcggcgg gtaattgccc ttgtaggggt
     1801 ggatgccgac gaacaattgg aacgagacga cggcgaacga gaaccagtcg gagccggtcg
     1861 taaagcttcg ggcgtgcctg tcccgcacgc tctccatcag caccgtcgcc gggtacgacg
     1921 cggtctggta gctgtcaacg tcgatgaagt aaacctccga gaaatcctcc gcgaccaaaa
     1981 agttcagctc gttcaagtcc acgaccagaa cgcctccggc gtggacgtgg ccgacgccct
     2041 cttgcagtct gcgcacgagg cggaaggccg actcgggcgt caggttatgt cgccggcgga
     2101 aggctttcgg gaagagctgg cagagcgcgt acgacctgcc gacggcgcgc atggaatagc
     2161 cgaccggctt gctcttcgtg tcgagcagca ctcccaaagg gcggatgatg ttgggcagag
     2221 tcagggccga gagttcgagg attttcgcgg gcgggatggc gcgccgcggg tcggtataaa
     2281 ttttataggc gttcgggcct ttgacgtaga tcgccccctc gccgccctgc gctttgaagt
     2341 cggactggcc cagccgaacc tcgcccccgc cctgaacgaa ataccggccc gccgcggcgt
     2401 cgcctttgag aagggcgccg aggcccggaa agacggcttt caacgccatg ctcaacctcc
     2461 cgtatgcagc gccccgaccg cgaggtcgtc cgcgtgttgc caacccttcc gccggcagtc
     2521 tttcagaaac ctcttcatgc ggcgcccgac gaaagcgccc cgtgtggttt tgaaggagac
     2581 gaggtcgcgc agcaccaagt ccaacgggac aggctcgact ccgccgccgg cctcggtctg
     2641 tccggcgcga aagaaggaat ggatgccgtc ggagaagagc gccgcgcatt cgtagtcggc
     2701 ggccttgacg gtaaacacct cggtcggcgg gccgcccgcc gtcgcgcccc tcggttgcaa
     2761 gcggtcttcg accgaagccg agcggtaatg tctcacctct ttacacgcgc gccccgcgtc
     2821 ccccagcgcg agcagccgct cgggctggtg cgtgtagctc gggtagagcg ggtagccgga
     2881 agggtaggcg atgctgtaaa cgtccagcgc gccctcccga gtctgtaagc agaccacgcc
     2941 gtccccggaa cagccgatgg ccagctcctc gccgtcgaga tgtgcggtga gcagcgtggc
     3001 gtccaccgcc tgcgagcgca ggcccagcaa ctcggcccac tccagcgcga gacgcgcggc
     3061 ctcttcgtgt atctccgcga ggtcgcggcc cggcggcccg ccgtgctcgc gcagcgtccg
     3121 ttcggcggct ttaaccatga gccgcgcgcc gatgtccgtg tcgggtgaag acgagcagcc
     3181 gtcggacagg atgacgtaag ggcgcgggcc gcgcgattcc gcatgcgcgg acggcggcca
     3241 cgcccccgcc agcgaatagt cctgacagac cgcgtgcgac gaccctatcc gaaaagcact
     3301 gtcggcattc atccgaatct ctcccgctgc cgtgcgcggc gacgccgttg attccggaag
     3361 cgtccccgcc cggagcgatt gaacgtccct caggcgacga agcctgccag tgcgtgaact
     3421 atccctcgaa acaggtggtc tttcgcctgc tgctccggcg gaagctgctc gaaggggacg
     3481 atgcacggat gggttttggc ggcggcgtcc ttcgactcgc cgtagaccca tccggtctcg
     3541 gctttctcct tcaaccagat gtcgtgtgtg gccgaagccg aggcgtccgg gttcgccaga
     3601 tggaattgaa cgcccttgat ggcggactcg cgctgccatt cgggagcctc gccccaagac
     3661 gtttgcgagt ggtcgccgac cgagtgacaa agcgacgcgt tcgcatcgtg gcaaattctt
     3721 gcgatctgtt cgatggtcat aagtctcctt tcgtaaccgc acaaagtagc cccgccctcc
     3781 ggcggcgagg gaggattcgc cgaagagaat ccctgtcagg tagagtcggc gggaagagcg
     3841 ttaaccgaac cgggccggcg cgggttagtg ccctcccgcc gaagggcgcc gtttagaaga
     3901 cgagcggctg cgaaggcccg cccgtgccga gcgcctgcga ctgtgcgctg atgctccgcg
     3961 agacgaactc ggcgaggcgc gcgagcgtct tggcgtcggc cttgtcgagt tcgacgtact
     4021 gcgtgaaacc cgcgtccgcg tggaactgcc ggaggaagcg agcggcctgc gggtcttgca
     4081 cgttgacgcc gatcaggacg gagaccatcg attcgagcgc ctcgctggtg acggccttcg
     4141 tgagcgcctc tttgactttc ccggcgggca gccgcgaggc gttgtccatc ccgtcggtga
     4201 tgacgaacag gatggcgttc gcggagaagt ccccggccag aagctgctgc ccgtagttgg
     4261 tggtcgagga gacggcgttc tccgccgcgt cgtagagggc ggtcgagccg cccgcgcgca
     4321 gcaccccgcc gtaatcggcg aggttgcagt tctccagcag cttgaagccg tgcagctcga
     4381 cgagcgcgtc gtcgaaggcc acgaggcgga gcagcaggtt gtcggcccgc gggctggact
     4441 tgcacgcctg cacgatgcgt gtgatggccg cctccatgtc gaagacgaag gcggcggtgc
     4501 tcccgctcac gtcgcaggcg accgtgacga ccgtgtactc ggtcgcgccc agctcctcca
     4561 ggcgtgtggc ggagtaaccg tagtggctgt tggggagtgt gacttggtca aggctcgtgt
     4621 cgttcagtat cggcatcgtc cgaggctcct ttcgtcaggc cagaaatgcg gtcgtggtcg
     4681 agagctgcat gccgcgcccg accatctcgc tcatgaagtt ttgggcgtgc gcctcgaagc
     4741 cggggatgtc gctggaggcg tcggtgagca gcacgagctt cgagacgaac gagtcgtcgc
     4801 cgaagctgtt ggcgatatca cgcacggtgt tggccagaca gtgcgtgcgc gcctcgccgg
     4861 cgatggcgac gaggtcggcg ttctccaaag tctggatgag gcgcgtgttg atctgcgtcg
     4921 aggcatccga ggggtcgggc acgtcggcct gcaccgccga gtagtgctcg gtcaggatgt
     4981 tgctgccctt ggtgacgtag tccacgaagg cgaagcgcga ctcccactcg gtgagcgcgg
     5041 cgaagagcgg ggggaagacc gcgtggccgg ggctgccgat gaggcagtgc ggcggccaga
     5101 ccgtcagctc gtagcgcccg ttctgttcga gccggcggac gtagtcgagc gcgcgccggt
     5161 acatgccggg caccgtggga gaccagacgc cctcctcgac ttcggcgcgg ctgatccggg
     5221 tgaagaccgg cgggttcgcg cccttcgagt ccttccagaa gatcgggtgc gcgatgtgta
     5281 tgaagtggtg cgagtcgagc gtgacgtgta tgtcgtcgag cttgttcctg aggcggcgca
     5341 ccatcgccgc cagccgtccc atgtcctgct cggcgccggg cacgtagagc gcgccgcgct
     5401 gcgggtcgca gaagtccacc tgcgggtcga tgatcagtag ctcgatcctc ttgctcattg
     5461 attgacctcc tcatggcctg cttagcgtaa aactgacgct aaggattaag ggcaaaaaaa
     5521 tttacacggc gccggtcgtc gtcgccgacc agagtccgaa actgaggccg cgcttgacga
     5581 gctgccggta cttgcgctcg tcgaatttgt agaggcgcgc ggcgcggtgc ggcacccctt
     5641 tctgcatctc gtccagctcg cgcagcacgt ccatgccgaa aatcttcttc ctgaagttgc
     5701 ggttgtccaa ctgcgtgccg agcacgatct cgtacagctt ctgtaactgc ttgatcgtga
     5761 acttgcgcgg gagcagctcg aagccgatgg gcgcgtaggt cagcttcccg cgcaccctgt
     5821 cgagcgcggt cttcagtatc tcggcatggt cgaaggccag acgcgggagc gagccgaccg
     5881 ggaaccagcg ggcttgccgc gcgtccgtcg cggcgtggat cgtgtgtttg tccggggaga
     5941 ccagagcata gtaggccacg gagacgaccc acccgcgcgg gtcgcgcgcg gggtcgccga
     6001 acgtgtacag ttgttccaga aagacctgcg cgatgccggt ctcctcctgc aactcgcgcg
     6061 tggccgccgc ttcgagcgtt tcgccgcgcc gcacgaagcc gccggggatc gcccacatcc
     6121 cggcgaaagg ttcgaggtct ctttccacga gcatgacctt cagcgtctct tccac
//
LOCUS       DBNGOH010001047         3703 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_891035, whole genome shotgun sequence.
ACCESSION   DBNGOH010001047 DBNGOH010000000
VERSION     DBNGOH010001047.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3703)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3703)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3703
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_891035"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(87..3308)
                     /locus_tag="AC159P_20360"
     CDS             complement(87..3308)
                     /locus_tag="AC159P_20360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017324258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3697853.1"
                     /translation="MKAPSRIANPFPGLRPFETEEYLLFFGREGQADELLARLGRTRF
                     LAVVGTSGSGKSSLVRAGLLPSLRGGLMREAGSGWRVALMRPGSDPVGNLAAALAERG
                     VLAEAGGGLEGAEAEAVIEATLRGGSLGLVEAARQARLAAHENLLVVVDQFEELFRFR
                     AVRAGGADTSDDAAAFVKLLLEAARQRELPVYVVLTMRSDFLGDCAQFQGLPEAINDG
                     QYLIPRMTRDERRVAITGPAMVARGRVAEPLVNRLLNDVGDNPDQLPILQHALMRTWD
                     YWKQHRRDSEPVSVEHYEAIGTMADALSRHADEAWYELADEHQRTIAERLFKALTEKG
                     ADNREIRRPASVREIAEITGASEAEVVAVVDTFRRAGRSFLMPPAEVRLDSDSVIDIS
                     HESLIRNWERLKKWVDEEAQAARNYRRLAESAVLHREGQEGMLNPVALQNALEWQRRN
                     MPNAAWGARYHPEFDLGMKYLGESKAHREEEAARVEEHRRAELERERREREQAERYAE
                     EQRRAARRQRRLSRGMGVLFLVTFGIAVVAVWLGRSNIKAREANDKFRDAVAAAQRGD
                     YDEAQRQLAKAIEGYQSRAVDNEEAVADSHAQLGNILFDRLYTEYTAAGGEPQSDEAR
                     AQLPQLVANYEEAANIYRGPDVEAPGKAAAALYTLGDMLMRLATENFSQRGASVTGIA
                     AARQRRGPSLTKERLPDLRLETTFTQSTRLAYDPDRAAQLKSDAAARFVQAYDLYRQA
                     LERYRSERDHDRRDEMDAGINAAREGMKRSAFRLGNYYVTEAIAAADSADPAQAGELW
                     RRAAERFAQLLPLHEGGDPSVALVLAWLGGVHEEMGDDAGAAGYYERVRRVALALPEK
                     PSEADVQFSFGAANFELGLKKSAAARFEEALEEYERLAAARAGDKGEAAQINFRKARA
                     YNALGLIYEQASDDETYARAVSSYWRAIEHYGYAFEAAPGAGPYSSDDLRAIGVFLKD
                     AADYGSALDALGLALRFAAGGGDKLAQARAQEETGAVYTLRRETRPALVAYEKAAKLY
                     EQLAQDTTDDDDTRARMQDELDRVNRIVSAIRSPSPARARAGR"
     gene            complement(3305..>3703)
                     /locus_tag="AC159P_20365"
     CDS             complement(3305..>3703)
                     /locus_tag="AC159P_20365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697854.1"
                     /translation="ENRDRSLVRPIKEYLFKQNIEVITWFDGDSAATLMDYHHKNLRE
                     CDAALVYFGNGDEPWVRKNLEDLEKAYGYGREDDWAASAVYVGAPPKDEKEDFLTHMA
                     SVIRNVAGFNPEDLREFVSAVEEAENGGTP"
BASE COUNT          463 a         1265 c         1356 g          619 t
ORIGIN      
        1 ggatgtcata acgcgccgca ggcgcgcgcc acgcgagggt tgatgaaggg aacgctgacc
       61 gaaagtcagt gcggcagtgc gtcgcgttag cgcccggcgc gcgcgcgcgc gggcgagggc
      121 gagcggatgg cgctgacgat gcggttgacg cggtcgagct cgtcctgcat ccgggcgcgc
      181 gtgtcgtcgt cgtcggtcgt gtcctgcgcg agctgttcgt agagtttggc ggccttctcg
      241 taggcgacga gggcggggcg ggtctcgcgt cgcagcgtgt agacggcgcc ggtctcttcc
      301 tgggcgcggg cctgcgcgag cttgtcgccg ccgccggccg cgaacctgag cgcaaggccg
      361 agcgcgtcga gcgcggagcc gtagtcggcc gcgtccttga ggaagacgcc gatggcgcgc
      421 aggtcgtccg aggagtacgg cccggcgccg ggcgcggcct cgaaggcgta cccgtagtgc
      481 tcgatggccc gccagtagga ggacacggcc cgcgcatagg tctcgtcgtc gctcgcctgc
      541 tcgtaaatca ggccgagcgc gttgtaggca cgcgccttcc tgaagttgat ctgggccgcc
      601 tcgcctttgt cgccggcgcg ggccgcggcg agccgctcgt actcttcgag cgcctcctcg
      661 aagcgggcag ccgccgactt cttcagcccc agctcgaagt tcgccgcgcc gaaggagaac
      721 tgcacgtcgg cctcggacgg cttctccggc agggccaagg cgacgcggcg cacgcgctcg
      781 tagtagccgg cggccccggc gtcgtcgccc atctcctcgt ggacgccgcc gagccaagcg
      841 agcacgagcg cgaccgacgg gtcgccgccc tcgtggagcg ggaggagctg tgcgaaacgc
      901 tcggcggccc ggcgccacaa ctcgccggcc tgcgcggggt cggcgctgtc ggcagccgcg
      961 atggcctcgg tgacgtagta gttgccgagg cggaaggccg agcgcttcat gccctcgcgg
     1021 gccgcgttga tgccggcgtc catctcgtcg cggcggtcgt gatcgcgctc ggagcggtag
     1081 cgttcgagcg cctgccggta gaggtcgtac gcctgtacga agcgcgcggc cgcgtcgctt
     1141 ttgagctgtg cggcgcggtc cgggtcgtag gcgaggcggg tggactgggt gaaagtggtc
     1201 tccagacgca ggtcggggag ccgttccttg gtgaggctcg gccctctgcg ctgacgcgcg
     1261 gcggcgatac ccgtgacgct cgcgccgcgc tgcgagaagt tctcggtggc gagccgcatg
     1321 agcatgtcgc cgagcgtgta gagcgcggcg gcggccttgc cgggcgcctc cacgtcgggg
     1381 ccgcggtaga tgttggccgc ctcttcgtag ttggcgacga gttgcgggag ctgcgcgcgc
     1441 gcctcgtcgc tctgcggctc gccgccggcc gcggtgtact ccgtgtagag ccggtcgaag
     1501 aggatgttgc cgagctgcgc gtgcgagtcg gcgacggcct cttcgttgtc cacggcccgg
     1561 ctctggtagc cttcgatggc tttggcgagc tggcgctgcg cctcgtcgta gtcgccgcgc
     1621 tgcgcggccg cgacggcgtc gcggaacttg tcgttggctt cgcgcgcctt gatgttgctg
     1681 cgcccgagcc agacggcgac gacggcgatg ccgaacgtga cgaggaacag cacgcccatc
     1741 ccgcgcgaga gccggcgctg gcggcgggcc gcgcgccgct gctcttcggc gtaacgctcg
     1801 gcctgctcgc gctcgcggcg ctcgcgttcg agttcggccc gtcgatgctc ttctacgcgc
     1861 gcggcctcct cttcgcggtg cgccttgctc tcgccgaggt acttcatgcc gaggtcgaac
     1921 tcggggtggt aacgcgcgcc ccacgcggcg ttcggcatgt tgcgccgctg ccattcgagc
     1981 gcgttctgca aggcgacggg gttgagcatg ccctcctgcc cttcgcggtg gagcacggcg
     2041 ctctccgcga gacggcggta gttgcgcgcg gcctgcgcct cttcgtccac ccacttcttc
     2101 agccgctccc agttgcggat cagactttcg tgcgagatgt cgatgaccga atcggaatcg
     2161 aggcggactt cggcgggcgg catcaggaac gagcgccccg cgcggcggaa cgtgtccacg
     2221 acggcgacga cttccgcctc gctcgccccg gtgatctccg cgatctctct cacactcgcc
     2281 gggcggcgaa tctcgcggtt gtcggcgccc ttctccgtga gcgccttgaa cagcctctcg
     2341 gcgattgtcc gctgatgctc gtccgccagc tcgtaccacg cctcgtcggc gtggcgcgag
     2401 agcgcgtccg ccatcgtgcc gatggcctcg tagtgctcga ccgagaccgg ctccgagtcg
     2461 cggcggtgct gcttccagta gtcccacgtc cgcatcagcg cgtgctggag gatgggcaac
     2521 tggtcggggt tgtcgcccac gtcgttcagg aggcggttga ccagcggctc ggcgacgcgc
     2581 ccgcgcgcga ccatcgcggg gccggtgatg gcgacgcgcc gctcgtcgcg cgtcatgcgc
     2641 gggatgaggt actggccgtc gttgatggcc tcgggcaggc cctggaactg cgcgcagtcg
     2701 ccgaggaagt ccgagcgcat cgtgagcacg acgtagacgg gcagctcgcg ttgccgcgcg
     2761 gcttcgagca gcagcttgac gaaggccgcg gcgtcgtccg acgtgtccgc gccgccggcg
     2821 cggaccgcgc ggaagcggaa cagctcttcg aactgatcga cgacgacgag caggttttcg
     2881 tgcgcggcga gacgcgcctg ccgcgcggcc tcgacgagtc cgagcgagcc gccgcgcaag
     2941 gtggcctcga tgacggcctc ggcctccgcg ccttcgagtc cgccgcctgc ctcggcgagc
     3001 acgccgcgct cggccagcgc cgccgcgagg ttgcccacgg ggtcgctgcc cggtcgcatc
     3061 agcgcgacgc gccagcccga gccggcctcg cgcatcagcc cgccccgcag cgaaggcagc
     3121 agccccgcgc ggacgagcga agacttgccg ctgcccgacg tgccgacgac ggccagaaag
     3181 cgcgtgcgcc cgaggcgcgc gagcagctcg tccgcctgcc cctcgcgccc gaagaagagc
     3241 aggtactctt cggtctcgaa gggccgcagg ccggggaagg ggttggcgat gcggctcggc
     3301 gccttcatgg cgtccctccg ttctcggcct cttcgacggc gctcacgaac tcgcgcaggt
     3361 cttcggggtt gaacccggcg acgttgcgga tgacggaggc catgtgcgtc aggaagtctt
     3421 ccttctcgtc cttcggcggg gcgccgacgt agacggcgct cgcggcccag tcgtcctcgc
     3481 gcccgtagcc gtaggccttc tccaggtctt cgaggttctt gcgcacccac ggctcatcgc
     3541 cgttgccgaa gtagacgagt gcggcgtcgc attcgcgcag gttcttgtgg tggtagtcca
     3601 tgagggtggc ggcgctatcg ccgtcgaacc aggtgatgac ctcgatattc tgtttgaaga
     3661 gatactcttt gatcggtcgc accagagatc gatcgcggtt ctc
//
LOCUS       DBNGOH010001048         2229 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4008963, whole genome shotgun sequence.
ACCESSION   DBNGOH010001048 DBNGOH010000000
VERSION     DBNGOH010001048.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2229)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2229)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2229
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4008963"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..296
                     /locus_tag="AC159P_20370"
     CDS             <1..296
                     /locus_tag="AC159P_20370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697855.1"
                     /translation="KKRAERLKDNLVLPEVAEGEKAPARAAPTDPAQLRTTLSRLDEL
                     ILRFANNLHAKGVRRFDAQTSSKLRRDLEEIVALGGQVKKCSEKLEKAAPQTR"
     gene            430..2196
                     /locus_tag="AC159P_20375"
     CDS             430..2196
                     /locus_tag="AC159P_20375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697856.1"
                     /translation="MLAKLRLVLLPAVCLTVGWTGPDGRGTAQAQAAHDAPRQTAATV
                     VIDDSALWGRLPLKDQVADAESLKVPWLFNNTALLLLSQASMETEVLIPEAGTYHLFA
                     RSRGGRRGSFRVALGKQVSPSIAGGDAFSWQRVEKFDLPQGSVKLRLTRIFTAPALDV
                     LVLTKNENFTEEELRPRQLHEEVKLLKEYRVPRPLAVNFGDANDDGKLDFIVMSEGFS
                     AHVFDHDGRELWAYEAPQEGADVRNVDDINGLIYDLDADGRSEVLHWRHMEGGEWLVV
                     ADGRSGRVKHRTKWPAPPLPHPYWNTRIAVARFAPGHPNNVVAYSDTGGTITVAAYTK
                     DLKPLWSHTEKKQKDHLGHYTYPFDIDGDGIDEIVASAIVLDAAGKVVWHRFDLFDDN
                     HDHADQIRFVDLDGDGRLELVAPWSDVGVVALDARSGALLWWHQAEHTQRLQTGSYLA
                     GVPGPHLAASARFYGNRQFEPYLWAQVHWFDAKGRLLSKWPANPINCNPVFVKGDWRG
                     DGKEELFWHLFRMKNDGEGVLYFGEPVYHMFDIVGDRAEEVVTLQGDRLRVYGYARAN
                     HAGERVKRDTEYWRTRLANHDH"
BASE COUNT          433 a          708 c          760 g          328 t
ORIGIN      
        1 taaagaagcg tgccgagcgg ctcaaggaca acctggtgct gcccgaggtg gcggagggcg
       61 agaaggcccc cgcgcgcgcg gccccgacag accccgcgca gttgaggacg acgctctccc
      121 ggctggacga gttgatactc aggttcgcga acaacctcca cgcgaaaggc gtcagacgtt
      181 tcgacgccca gacgtcgtcc aagttgcggc gtgacctgga ggagatcgtc gcgctgggcg
      241 ggcaggtgaa gaagtgcagc gagaagctgg agaaggccgc cccacaaaca cggtgaacgc
      301 agacagcggc ccgtcgagga ccaggcacgg tgaggagggc ggacgggcga agcagcgctg
      361 ctctccaccg accacaaaac gcgcgccgac cacgcgcacc caaccttcag tcgatagagg
      421 aacacgtcga tgctggcaaa actccgtctc gttctgttgc cggcggtgtg tctgaccgtc
      481 ggttggacgg ggccggacgg acgcggcacg gcgcaggcac aggccgccca cgacgcgccg
      541 cggcagacgg cggcgacggt cgtgatcgac gactcggcgc tgtggggcag gctaccgctg
      601 aaagatcagg tcgccgacgc cgagtcgctg aaagtcccgt ggctgttcaa caacacggcc
      661 ctcctgctac tgtcccaggc aagcatggag accgaggtgc tgatcccgga ggcgggtacg
      721 tatcacctct tcgcgcggag cagagggggg cggcgcggtt cgttcagggt cgcgctcggc
      781 aagcaagtct cgccgtccat cgcgggcggc gacgcctttt cgtggcagcg ggtcgagaag
      841 ttcgacttgc cgcaaggctc tgtcaagctc cgcctcacgc ggattttcac ggcccccgcc
      901 ctcgacgttc tggtgctcac gaagaacgag aacttcacgg aagaggagct gcgccctcgc
      961 caactgcacg aagaggtgaa gctgctgaag gagtacaggg tgccgcgtcc gctggcggtc
     1021 aacttcggcg acgcgaacga cgacgggaag ctggacttca tcgtcatgtc ggagggcttc
     1081 tcggcgcacg tcttcgacca cgacgggcga gagctgtggg cttacgaggc cccgcaagag
     1141 ggggccgacg tgcgcaacgt tgacgacatc aacggcctca tctatgacct ggacgccgac
     1201 gggcgttcgg aggtgctgca ctggcggcac atggagggcg gcgaatggct cgtcgtcgcc
     1261 gacggaagaa gcggtcgggt gaagcacagg acgaagtggc cggcgccccc tctgccgcac
     1321 ccttattgga acacacggat cgccgtcgcg cggttcgccc ccggccaccc gaacaacgtc
     1381 gtcgcctaca gcgacacggg cggcaccatc acggtcgccg cctacacgaa ggacttgaag
     1441 ccgctgtggt cgcacaccga gaagaagcag aaagaccatc tcgggcatta cacctacccg
     1501 ttcgacatcg acggcgacgg gatagacgag atcgtcgcca gcgccatcgt gctcgacgcc
     1561 gcgggcaagg tcgtgtggca ccgcttcgat ctgttcgacg acaaccacga ccacgccgac
     1621 cagatacgtt tcgttgacct cgacggcgac gggcggctgg agctggtggc cccctggagc
     1681 gatgtcggcg tcgtggcgct cgacgcacgg agcggcgcgt tgctctggtg gcatcaggcc
     1741 gagcacacgc aacggcttca gaccggcagc tacctcgcgg gcgttccggg gccgcacctc
     1801 gcggccagcg cccgcttcta cggcaaccgg cagttcgagc cgtacctgtg ggcgcaggtt
     1861 cactggttcg acgccaaagg cagactgctc tcgaagtggc ccgccaaccc catcaactgc
     1921 aacccggtct tcgtcaaagg cgactggcgc ggcgacggca aagaggagct gttctggcac
     1981 ctcttcagga tgaagaacga cggcgagggc gtgctctact tcggcgagcc tgtctatcac
     2041 atgttcgaca tcgtcggcga ccgggccgag gaagtcgtca cgcttcaggg cgaccggctc
     2101 agggtatacg gctacgcgcg ggcgaatcac gccggtgaga gggtcaagcg cgacaccgaa
     2161 tactggcgca cgcgactggc gaaccacgat cattaaggat gtttgcggtt gagtgtaccg
     2221 acggcagga
//
LOCUS       DBNGOH010001049         5211 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4741385, whole genome shotgun sequence.
ACCESSION   DBNGOH010001049 DBNGOH010000000
VERSION     DBNGOH010001049.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5211)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5211)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5211
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4741385"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..681
                     /locus_tag="AC159P_20380"
     CDS             <1..681
                     /locus_tag="AC159P_20380"
                     /inference="COORDINATES: protein motif:HMM:NF019344.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR3697857.1"
                     /translation="MPRGIVGSERGGVLTEQLRENPYTVLLLDEVEKASPNLMNLFLQ
                     AFDEGWLTDGRGKKVYLSDAIVIMTSNLGSENFKKYEKPFGFGTKTLGDVKAIRGDVM
                     KAAEQRFSPEFRNRIDEIVVFSPLTMDEVRDIARLYLGKLQRQMASQGKILEVTEAAT
                     DLLTEKGYNPTYGARFLKRHIDEKVKLPITAMWKLAPRFVADVENGEVVIKMDEAAPA
                     FAADVEVV"
     gene            846..1694
                     /locus_tag="AC159P_20385"
     CDS             846..1694
                     /locus_tag="AC159P_20385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697858.1"
                     /translation="MKKKLCPLAPARALVTALFVSITAHAAFAQTASNAAPTATPAAK
                     EAPAKVDDKAEQVVARAVEALGGASFLGVRSITSRGYYTPYEQGVARVPVTFVNYLVF
                     PDRERTEFKGSGVRSIQTYAGETGWIFDGMVKKIKDLTPEQVEDFRFAMRTSLDNLLR
                     GWWREKGARLSYVGRREAGVGMRNEVVRLTYEDGFAVEYEFSARDGVPAKVRYKRKNK
                     EGEDVEEEERYAQYLSVNGVGFAHIQDRYSAGLQSSRVNYEKVELNAPVPESLFAKPA
                     DVKSLK"
     gene            complement(1616..2113)
                     /locus_tag="AC159P_20390"
     CDS             complement(1616..2113)
                     /locus_tag="AC159P_20390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697859.1"
                     /translation="MTKTNMNATDFIICCRRLVGALRQPPREGGHATFFLGFDEDGLP
                     FYIAGSFNAGQLTRAFTGPLFFCPFCGRRVRLGGNDSANAARGAARRAMTEDDDEELT
                     RLSLEETGEVETIYAPRRLHDSGARAGAGSFVGETPAPHFKDFTSAGLANSDSGTGAF
                     SSTFS"
     gene            complement(2161..2442)
                     /locus_tag="AC159P_20395"
     CDS             complement(2161..2442)
                     /locus_tag="AC159P_20395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697860.1"
                     /translation="MQLVYATGCRERDFLIAGSRNEVPAGARIYATCTRESTVEDVGE
                     ITFQSMSEDEYYSLKAMVREARERARAEKQSAGEDASPLRSLLGRFFSR"
     gene            complement(2674..3369)
                     /locus_tag="AC159P_20400"
     CDS             complement(2674..3369)
                     /locus_tag="AC159P_20400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3697861.1"
                     /translation="MLEQNANTQAMQQQPPPANGVVKVAIIEDLREIREGLATLIDLT
                     EGYRCTGRYGSVEEALARIARDVPDVVLSDIGLPGMSGIEGVRILKERYPHMLFLILS
                     VYDDDERVFDALCAGACGYLLKKTPPARLIESLKDAVEGGAPMSPEIARRVITLFRDF
                     RPPKRAEYDLTPHETRILKLLVEGHNYKTAATELGVTVNTVSFHLKKIYEKLQVHSKS
                     EAVAKALRDGLIK"
     gene            complement(3465..>5211)
                     /locus_tag="AC159P_20405"
     CDS             complement(3465..>5211)
                     /locus_tag="AC159P_20405"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3697862.1"
                     /translation="LFEDSRGDVWIGSYNPPVTLTRGERASEPFRRYSEADGIPAFNW
                     PNVFKEDRAGHVWIGLHNGGLMRWRGQRLEVFGVGGHVPEGIGQSLYLDRAGRLWYAA
                     STGGTLFADPAGEDPRPAPLPTDDLASLNLRGFAEDPFGRVYFATARGVDRLDPETGR
                     VKHFTTADGLIKSEVVAALRDRVGRMWFGTREGVSRLMPAPDVRGEPPPVLIGALSAG
                     GVAQAVAELGETEVPELVFGPSGGQVQIDYFGLSFAAGDAVRYQYKFEGAGGDWSAPT
                     EQRTVTANLSPGKYRFLVRAVGPDGATTGSPAGVSLNSLPPVWQRWWFLALAAIVSGS
                     VIYRVARARARRALLLERVRTRIATDLHDDIGASLSKIAILSEVVSHRVNADTNGAGS
                     PVAEPLATIAGTSREMVDSMSDIVWAINPKRDRLSDLAQRMRLFASDLLSARDIRFTF
                     RAPDASRDITVGADLRREVYLIFKETVNNLAKHSACEDAEIELRLAGSRLVVRVSDNG
                     KGFDHARGLAAGGEHSTMGGHGLHSMRGRAEKLGGTYEIDSARGRGTTVTLEVPVKVK
                     RRRRVLPRLKKLWRR"
BASE COUNT          816 a         1680 c         1849 g          866 t
ORIGIN      
        1 atgccgcgcg gcatcgtcgg ctcggagcgc ggcggcgtgc tcaccgagca gctccgcgag
       61 aacccgtaca cggtgctcct gctcgacgaa gtcgagaagg cttcgccgaa cctgatgaac
      121 ctcttcctgc aagccttcga cgagggctgg ctgacggacg gtcgcggcaa gaaggtttat
      181 ctgtcggacg ccatcgtcat catgacctcg aacctcggct cggagaactt caagaagtac
      241 gagaagccgt tcggcttcgg cacgaagacg ctgggcgacg tgaaggccat ccgcggcgac
      301 gtgatgaagg ccgccgagca gcgcttctcg cccgagttcc gcaaccgcat cgacgagatc
      361 gtcgtcttct cgccgctgac gatggacgag gtgcgcgaca tcgcgcggct ctacctcggc
      421 aagctccagc ggcagatggc ttcgcagggc aagattctcg aagtgacgga ggcggcgacc
      481 gacctgctga ccgagaaggg ctacaacccg acctacggcg cgcgcttcct gaagcgccat
      541 atcgacgaga aggtgaagct gccgatcacg gcgatgtgga agctggcccc gcgcttcgtg
      601 gccgacgtgg agaacggcga ggtcgtcatc aaaatggacg aggccgcgcc cgcgttcgcg
      661 gcggacgtgg aagtcgtcta aagaccgggc ctgtcgtcaa gaccggcccg cggacggttc
      721 gagggggctt caatgaatcg ctgcattgaa gccccctcgt ttatatcaac cgcgcggcga
      781 cgcgcccgcc gacacacggc ccggcccgtt gcttcgcgcc ctccccgcgt tctataaaac
      841 tcgacatgaa gaagaagctt tgccccctcg cccctgcacg cgcgctcgtg accgcgctct
      901 tcgtttcaat cacagcccac gccgctttcg cgcagaccgc gagcaacgcc gccccgaccg
      961 cgacgcccgc cgcgaaagag gcgcccgcca aagtagacga caaggccgag caggtcgtcg
     1021 cgcgcgccgt cgaggcgctg ggcggcgcga gtttcctcgg cgtgcgttcg atcacgtcgc
     1081 gcggctacta cacgccgtac gagcagggcg tggcgcgggt gccggtgacc ttcgtcaact
     1141 acctcgtctt ccccgaccgc gagcgcacgg agttcaaggg gagcggggtg cgcagcatcc
     1201 agacgtacgc gggcgagacg ggctggattt tcgacgggat ggtgaagaag atcaaagacc
     1261 tcacgcccga acaggtcgag gatttccgct tcgcgatgcg gacgagcctc gacaacctgc
     1321 tgcgcggctg gtggcgcgag aagggcgcgc gcctctctta cgtcgggcgg cgcgaggcgg
     1381 gcgtcgggat gcgcaacgag gtcgtgcgcc tcacctacga ggacgggttc gcggtcgagt
     1441 acgagttcag cgcgcgcgac ggggtgccgg cgaaggtgcg ttacaagcgg aagaacaagg
     1501 agggggagga tgtcgaggag gaggagcgct acgcgcagta cctctcggtg aacggcgtcg
     1561 ggttcgcgca catccaggat cgctacagcg cgggcttgca gtcctcgcgc gtcaactacg
     1621 agaaggtgga gctgaacgcg cccgtccccg agtcgctgtt cgccaagccg gcggacgtga
     1681 agtccttgaa gtgaggcgcg ggggtctcgc ccacgaagct ccccgcgccc gcgcgcgcgc
     1741 cagagtcgtg taggcggcgc ggcgcgtaga tcgtctcgac ctcgcccgtc tcttcgagcg
     1801 agaggcgcgt cagctcttcg tcgtcgtctt ccgtcatggc gcggcgcgcg gcgcctctcg
     1861 cggcgttcgc cgaatcgttg ccgccgaggc ggacgcggcg cccgcagaag gggcagaaga
     1921 agagcggccc ggtgaaggcc ctcgtcaact gcccggcgtt gaaactgccg gctatgtaga
     1981 acgggaggcc gtcttcgtcg aagccgagga agaaggtcgc gtggccgcct tcccgcggcg
     2041 gctgccgcag ggcgccgacg aggcggcggc agcagatgat gaagtccgtg gcgttcatgt
     2101 tcgtcttcgt cacaggcgtg tcacgttcgt catggtcaca ggcgcgacgc gcgcgccgcc
     2161 ttaccggctg aagaacctcc cgaggagcga gcgcagcggc gacgcatctt cgcccgccga
     2221 ctgcttctcg gcccgcgcgc gctcgcgcgc ctcgcgcacc atcgccttca gggagtagta
     2281 ctcgtcctcg ctcatgctct ggaaagtgat ctcgcccacg tcctcgaccg tgctctcccg
     2341 cgtgcaggtc gcgtagatgc gcgcgcccgc cggcacctcg ttgcggctgc cggcaatcag
     2401 aaaatccctc tcgcggcagc ccgtggcgta aacaagctgc atcgtccttc tccctctcct
     2461 cccgaagtct tcttcgtgtc accccccccg tcccgccccg acgacacact tttacgccgg
     2521 acgcgccctc gccgtcacca cgtatgtatg tagggaaaga agtaggcagt aggcagtagg
     2581 cagttaaagg aaagaggctt gagcgttaac ggctcaagcc tctttcctct tcggttttaa
     2641 ctgcctaccg cctactgcct actgcctact tcttcacttg atgagtccgt cgcgcagggc
     2701 tttggcgacg gcttcggatt tggagtgcac ctggagtttt tcgtaaatct tcttgaggtg
     2761 gaaggagacg gtgttgaccg tgacgccgag ttccgtggcc gcggtcttgt agttgtggcc
     2821 ctcgacgagg agcttgagga tgcgcgtctc gtggggcgtg aggtcgtatt cggcgcgctt
     2881 cgggggccgg aagtcgcgga agagcgtgat gacgcggcgg gcgatctcgg gcgacatggg
     2941 ggcgccgccc tcgaccgcgt ccttgaggct ctcgatgagg cgcgcgggcg gcgtcttctt
     3001 caacaggtag ccgcaggccc cggcgcagag ggcgtcgaag acgcgctcgt cgtcgtcgta
     3061 gaccgagagg atcaggaaga gcatgtgcgg gtagcgctct ttcaggatgc gcacgccctc
     3121 gatgccgctc atgccgggca ggccgatgtc ggagagcacc acgtcgggca cgtcgcgcgc
     3181 gatgcgcgcg agcgcctcct cgaccgagcc gtaacggccc gtgcagcggt agccttcggt
     3241 caggtcgatg agcgtcgcca gcccctcgcg gatttcgcgc aggtcttcga tgatggcgac
     3301 tttgacgacc ccgttcgcgg gcggcggctg ttgttgcatc gcttgcgtgt tcgcgttctg
     3361 ttcgagcatc ttcgggtcaa gcctcttctg cggcccattc aggatacgcg taagattaac
     3421 ccggaaggga cgggggcgat actacctatg tatgtagtaa ctttctaccg gcgccataac
     3481 tttttcagcc gcgggagcac gcgccgccgc cgcttgacct tgacgggcac ttcgagcgtg
     3541 acggtcgtgc cgcgtccgcg cgccgagtcg atttcgtaag tgccgcccag tttttcggcc
     3601 cgcccgcgca tgctgtgcag gccgtggccg cccatcgtcg agtgctcgcc gccggcggcg
     3661 aggccgcgcg cgtggtcgaa gcccttgccg ttgtcgctga cgcggacgac gaggcggctt
     3721 cccgcgaggc gcagctcgat ctcggcgtct tcgcaggcgg agtgcttggc gagattgttc
     3781 accgtctcct tgaagatgag atagacctcg cgccgcaggt ctgcgccgac ggtgatgtcg
     3841 cgcgaggcgt cgggcgcgcg gaaggtgaac cggatgtcgc gggcgctcag gaggtcggag
     3901 gcgaacagcc gcatgcgctg cgcgaggtcg ctcaggcggt cgcgcttggg gttgatggcc
     3961 cagacgatgt cgctcatcga atcgaccatc tcgcgcgagg tgccggcgat ggtcgccaga
     4021 ggttccgcga ccggcgagcc ggcgccgttc gtgtcggcgt tgacgcggtg gctgacgacc
     4081 tcgctcagga tggcgatctt cgagaggctc gcgccgatgt cgtcgtgcag gtcggtggcg
     4141 atgcgcgtgc ggacgcgttc gagcaggagg gcgcggcggg cgcgggcgcg ggcgacgcgg
     4201 tagatgacgg agccggagac tatggcggcg agcgcgagga accaccagcg ctgccagacc
     4261 ggcgggaggc tgttgagaga gacgccggcg gggcttccgg tggtcgcgcc gtcggggccg
     4321 acggcgcgga cgaggaagcg gtacttgccg ggcgagaggt tggcggtgac ggtgcgctgt
     4381 tcggtcggcg cgctccagtc gccgcccgcg ccttcgaatt tgtattggta gcgcaccgcg
     4441 tcgcccgccg cgaagctcag gccgaagtag tcgatctgca cctgcccgcc cgaaggcccg
     4501 aacacaagct cgggcacttc ggtctcgccc agctcggcca ccgcctgcgc gacgccgccc
     4561 gcgctcaagg ccccgatgag gacgggcggc ggctcgcctc gcacgtcggg cgcgggcatc
     4621 aggcgcgaga cgccttcgcg cgtgccgaac cacatccggc ccacgcggtc gcggagggcg
     4681 gcgacgacct cgctcttgat gaggccgtcg gcggtggtga agtgtttgac gcggcccgtc
     4741 tccgggtcga ggcggtcaac gccgcgcgcg gtggcgaagt agacgcgccc gaaggggtct
     4801 tcggcgaagc cgcgcaggtt gaggctggcg aggtcgtcgg tcggcagcgg cgcggggcgc
     4861 gggtcttcgc ccgcggggtc tgcgaagagc gtgccgccgg tgctcgcggc gtaccagagg
     4921 cggcccgcgc ggtcgaggta gagcgactgg ccgatgcctt cgggcacgtg gccgccgacg
     4981 ccgaagactt cgaggcgctg cccgcgccag cgcatcagcc cgccgttgtg cagcccgatc
     5041 cagacgtggc cggcgcggtc ttccttgaag acgttcggcc agttgaaggc ggggatgccg
     5101 tccgcttcgg agtagcgccg gaagggttca ctcgcgcgct ccccgcgcgt gagcgtcacg
     5161 ggcgggttgt aagagccgat ccacacgtcg ccgcgcgagt cttcgaagag g
//
LOCUS       DBNGOH010001050         7107 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2174537, whole genome shotgun sequence.
ACCESSION   DBNGOH010001050 DBNGOH010000000
VERSION     DBNGOH010001050.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7107)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7107)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7107
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2174537"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1351
                     /locus_tag="AC159P_20410"
     CDS             <1..1351
                     /locus_tag="AC159P_20410"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3697863.1"
                     /translation="SLWGHQLGAILWALVVTATLKTLANVALPLAGVYTLLPVGSIFV
                     LLGVVIYAYAITNFKLFSLQSALDQFRLCPIAYKVALAIAAVAVTSFVLLQVPVVWWS
                     FTGQTPDAWKRYLVFSVVTALLPNLILVALVIRIISRPLRRLTEAAVDVAGGAYGTQV
                     NLKSNDEVGLLAESFNGMSRKMADDIERLRALNEHLVRTEKLAATGTLAAGVAHEVNN
                     PLASISSLIQMLQARPLTAENEAETREMLRLAQTQIARITQVLRDLMDFARVQPPARA
                     PLDVARVVEASIRLASFDKAFKRLRLSTDFDPAAPHVAADTDQLQQVFLNLLLNARDA
                     MPDGGDLRVTTTFVEQAREVVVEIADTGHGIAPDTLPHVFDPFFTTKPAGAGTGLGLA
                     VCYGIVTAHGGRIEVEPNNGHGTRVQVTLPAHEARAAAAGPPRQRAPYPEGQTVRGN"
     gene            1357..1923
                     /locus_tag="AC159P_20415"
     CDS             1357..1923
                     /locus_tag="AC159P_20415"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020154374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3697864.1"
                     /translation="MFIHRIDDEIFLRLPEERHSPEAYALIMRVAEYLREWLPWVTED
                     FSLEDAKEFVRRTRRQFANNEGYSAQIVYRDRIAGQVGFNGFDWPNSKTEIGYWLGAS
                     FQGRGIMTRACRALVNHAFRELGFNRVEIKCAVGNARSRAIPARLGFTEEGTLRQAER
                     LHDRYVDLVVYGLLAAEWPQKGATDAGR"
     gene            1982..2443
                     /locus_tag="AC159P_20420"
     CDS             1982..2443
                     /locus_tag="AC159P_20420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010194791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR3697865.1"
                     /translation="MPLIRLETLIRAPAELCFDLSRDIDVHAASTDGTGERAVAGVTS
                     GLINLGETVTWEATHFFVRQRLTSKITAFDRPHMFVDEMQRGAFKRWHHTHLFEVRDD
                     GTLMVDEVEYASPLGLLGQLVDALVLKKYMTRLLSERNAHIKKLAEQTTRT"
     gene            2440..3807
                     /locus_tag="AC159P_20425"
     CDS             2440..3807
                     /locus_tag="AC159P_20425"
                     /inference="COORDINATES: protein motif:HMM:NF025885.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HIR3697866.1"
                     /translation="MNTKPLILVAEDEVVLREQVISRLLSEAGYRVVAVESAELAREV
                     FATEDVAVTLTDIRMAGMDGLALLDRIKDVDAEALLIVMTAYSSVDSAVAALRKGAFN
                     YIVKPFLNEDLLNSIKIALRQRELFRENRYLRRELDRRYSFSEIIGTSDSLQRVFRLV
                     EKVAPTNTNVLIQGESGTGKELIARAIHHNSARSMRPFVAINCGALPETLLESELFGH
                     VKGAFTGAVASKPGLFRAAEGGTVFLDEIGEVTPAMQVRLLRAVQEHEVTPLGASTPV
                     QFDARIISATTRDLARDVAATRVREDLFYRLNIIELHLPPLRERREDIPLLVRHFVKH
                     TAREQQHDEKAIDPQAMTAFLNYTWPGNVRELQNAVERAFTLSGELIDLDSLPPRVRD
                     AAAKAGPDAMRDPDGLRPTLSEIERRHILDTLASVNQDKARAANILGIDLSTLYRKLK
                     RYDAV"
     gene            3892..4740
                     /locus_tag="AC159P_20430"
     CDS             3892..4740
                     /locus_tag="AC159P_20430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020595480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HIR3697867.1"
                     /translation="MWRYLREALNAALIALGILSAAMGLKGFLLSSRFIDGGVTGVSM
                     LLADVTGVPLAVLIPVINLPFIALGYRQIGKKFAIKSALAIAGLALCLAFVKFPDVTP
                     DKLLTAVFGGFFIGAGIGLAIRGGSVLDGTEVAALLVSKGSSLLKVGDVILVLNIFIF
                     SAAAFLLGVESAMYSILTYFAASKTIDFLIHGIEEYTAVIIISERSDEIRRAITDDLN
                     RGVTVYKGRGGMTGAEQDILYCVVTRLEIGRVRSVAHGIDGAAFVVVHPLSDVDGGVI
                     KRPALH"
     gene            complement(4755..5162)
                     /locus_tag="AC159P_20435"
     CDS             complement(4755..5162)
                     /locus_tag="AC159P_20435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697868.1"
                     /translation="MSWRGAGEGDEERRRGAGGTPGGVGEFLLGLAMAVAGAYILTSR
                     VTVTGGFWRVWGFNGFGLSLLPLIFGIGLLFFNGRSKAGWLLVFAGAVIILTGVLMNL
                     DIYFQPTSLFNTILMFVLLAGGIGLVARALRAH"
     gene            complement(5169..5402)
                     /locus_tag="AC159P_20440"
     CDS             complement(5169..5402)
                     /locus_tag="AC159P_20440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697869.1"
                     /translation="MDELVRQVAQKTGIAEEQARTAVTTVLGFLKERLPAPIAGQLDG
                     VISGGAGAVGGLTDKAGDVLGGLGGMLGGGGKE"
     gene            complement(5472..5738)
                     /locus_tag="AC159P_20445"
     CDS             complement(5472..5738)
                     /locus_tag="AC159P_20445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697870.1"
                     /translation="MNERDFFDERQETKRATYSCPHCRERGEYQVRWLRRTKKAQPPR
                     GMNEQQRAQFRNARDYMVRMDDMLVCGRCRKRFDIPNFQSVVFI"
     gene            complement(5848..6123)
                     /locus_tag="AC159P_20450"
     CDS             complement(5848..6123)
                     /locus_tag="AC159P_20450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011422456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET family transporter"
                     /protein_id="HIR3697871.1"
                     /translation="MVEAVGWVSSVILVLTIAKQVYKQWQEGSSEGVSKWLFVGQMAA
                     SLGFTVYSWLVDNWVFVVTNAVMLLNGLVGLLIVLHHRKKERPGAGD"
     gene            complement(6182..6541)
                     /locus_tag="AC159P_20455"
     CDS             complement(6182..6541)
                     /locus_tag="AC159P_20455"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3697872.1"
                     /translation="MKKILVVEDDEVSRELVRMTLARRGYAVTVAEDGMRGYEQAVEG
                     RPDLIITDVWMPAADGIHLVRRVRDTPEIAATPILVTTGFGTGSATLTLAQGADAYEP
                     KPLDPVTLLETVRRLLA"
     gene            complement(6660..6983)
                     /locus_tag="AC159P_20460"
     CDS             complement(6660..6983)
                     /locus_tag="AC159P_20460"
                     /inference="COORDINATES: protein motif:HMM:NF017922.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HIR3697873.1"
                     /translation="MSEVLRRGPAVEPREIMQETDARLRVTWGDGRACVYEAPRLRRL
                     CPCAQCVNEFTGERVLKPESVADELKIGNVELVGRYALTFRWSDGHDTGIYSFRYLRE
                     ICEGE"
BASE COUNT         1205 a         2533 c         2181 g         1188 t
ORIGIN      
        1 gagcctgtgg ggccaccagc tcggcgcgat cctgtgggcg ctcgtcgtga cggcgacctt
       61 gaagacgctc gccaacgtcg cgctcccgct cgcgggcgtc tacacgcttt tgccggtcgg
      121 ctcgatcttc gtcctcttgg gcgtcgtcat ctacgcctac gcgatcacga acttcaagct
      181 cttctcgctg caatcggcgc tcgaccagtt ccgcctctgc cccatcgcct acaaggtcgc
      241 gctcgccatc gcggcggtcg ccgtcacgag cttcgtcctg ctgcaagtgc cggtcgtctg
      301 gtggagcttc accggccaga cgcccgacgc ctggaagcgc tacctcgtct tcagcgtcgt
      361 cacggcgctc ctgccgaacc tgattctggt cgcgctcgtc atccgcatca tctcgcgccc
      421 cttgcgccgc ctgaccgagg ccgccgtcga tgtcgcgggc ggcgcctacg gcacgcaggt
      481 caatttgaag agcaacgacg aggtgggcct gctcgcagag tctttcaacg ggatgagccg
      541 caagatggcc gacgacatcg agcgcctgcg cgccttgaac gaacacctcg ttcgcaccga
      601 aaaactcgcc gccaccggca cgctcgccgc gggcgtcgcg cacgaggtca acaacccgct
      661 cgcctcgatc tcttcgctca tccagatgct acaggcgcgg cctctcaccg ccgagaacga
      721 agccgagacg cgcgagatgc tgcgtctggc gcagacgcag atcgcgcgca tcacgcaggt
      781 cttgcgcgac ctgatggact tcgcgcgcgt ccagccgccc gcgcgcgcgc cgctcgacgt
      841 ggcgcgcgtc gtcgaggcga gcatccgcct cgccagcttc gacaaagcct tcaagcggct
      901 gcgtctttcg accgacttcg accccgccgc gccgcacgtc gccgccgaca cggatcaact
      961 gcaacaggtc ttcctgaacc tgctcctgaa cgcgcgcgac gcgatgcccg acggcggcga
     1021 cctccgcgtc accacgacct tcgtcgagca ggcgcgcgag gtcgtcgtcg agatcgcgga
     1081 caccggccac ggcatcgcgc ccgacacctt gccgcacgtc ttcgacccct tcttcacgac
     1141 gaagcccgcg ggcgcgggca ccggcctcgg cctcgccgtc tgctacggca tcgtcaccgc
     1201 gcacggcgga cgcatcgagg tcgagccgaa caacgggcac ggcacgcgcg tacaggtcac
     1261 gctccccgcg cacgaggcgc gcgccgccgc cgccgggccg cctcggcaac gcgcgcccta
     1321 tccggagggc cagaccgtcc gaggaaactg acgcccatgt tcatccaccg catcgacgac
     1381 gaaattttcc ttcgactgcc cgaagagcgc cactcgccgg aggcgtacgc cctcatcatg
     1441 cgcgtcgccg aatacctccg cgagtggctg ccgtgggtga ccgaagactt ctcgctcgaa
     1501 gacgccaagg agttcgtgcg ccgcacccgc cgccagttcg cgaacaacga aggctactcg
     1561 gcccagatcg tctaccgcga ccgcatcgcc ggccaggtcg gcttcaacgg cttcgactgg
     1621 cccaactcca agaccgagat cggctactgg ctcggcgcct cgttccaggg ccgcggcatc
     1681 atgacgcgcg cctgccgcgc cctcgtcaac cacgccttcc gcgagctggg gttcaaccgc
     1741 gtcgagatca aatgcgccgt cggcaacgcc cggagccgcg ccatccccgc acgcctcggc
     1801 ttcaccgagg agggcacgct gcggcaggcc gaacgcctcc acgaccgcta cgtcgatctc
     1861 gtcgtctatg gcctgctcgc cgccgagtgg ccccaaaagg gagcgaccga cgcgggacgg
     1921 taaacgacgc gcggaagaca agccgcgccc gtttcggcgc tgtgcgccca ccatgagagt
     1981 tatgccgctc atccgcctcg aaactttgat ccgcgcgccc gccgagcttt gtttcgacct
     2041 ctcgcgcgac attgacgtgc acgcggcttc gaccgacggg acgggcgagc gcgcggtcgc
     2101 cggcgtcacg tcggggctga tcaatctggg cgagaccgtg acgtgggagg cgacccactt
     2161 cttcgtgcgg caacgcctga cctccaaaat cacggcgttc gaccgcccgc acatgttcgt
     2221 tgacgagatg cagcgcgggg ccttcaaacg gtggcatcac acgcacctgt tcgaagtgag
     2281 ggacgacggc acgctgatgg ttgacgaggt tgaatacgcc tcgccgctgg ggctgctcgg
     2341 ccagctcgtt gacgcgctcg tccttaagaa gtacatgacc cggctgctgt cggaacgtaa
     2401 cgcacacatc aagaagctgg cggagcagac gacacgaaca tgaacaccaa gcccttgatc
     2461 ctcgtcgccg aagacgaagt agtattgcgt gagcaggtta tctcgcgtct gcttagcgag
     2521 gcaggctacc gcgtcgtggc ggtagagagc gctgaattag cacgtgaggt attcgccacc
     2581 gaggacgtcg ccgttacact gaccgacatt cggatggcgg gcatggacgg cctcgccctg
     2641 ctcgaccgca tcaaggatgt tgatgctgag gcgctcctca tcgtgatgac cgcttactcg
     2701 tccgttgact ctgcggttgc cgcgctacgg aaaggtgcct ttaactacat cgtaaagcca
     2761 ttcttgaatg aagacctgct gaactcgatc aagattgcac tccgccagcg cgaattgttc
     2821 agggagaacc ggtatctgcg gcgcgaactc gaccgccgtt acagtttttc cgagattatc
     2881 ggcacgtccg actcacttca acgagttttc aggctcgtcg aaaaagtcgc acctactaac
     2941 accaacgtcc tcattcaagg cgaaagcggc accggcaagg agctgatcgc ccgcgccatc
     3001 caccacaaca gcgcgcgctc gatgcgcccc ttcgtcgcca tcaactgcgg cgccctgccc
     3061 gagacgcttc tcgaatccga gctgttcggc cacgtcaaag gcgccttcac cggcgccgtc
     3121 gcctccaagc ccggcctctt ccgcgcggcg gaaggcggca cggtcttcct cgacgagatc
     3181 ggcgaagtca cgcccgccat gcaggtgcgt ctgctgcgcg ccgtgcagga gcacgaagtc
     3241 acgcctttag gcgcgagcac gcccgtccag ttcgacgccc gcatcatctc ggccaccacc
     3301 cgcgacctcg cacgcgacgt cgccgcaacg cgcgtccgcg aagacctctt ctaccgcctc
     3361 aacatcatcg agctgcacct gccccctttg cgcgagcgcc gcgaagacat cccgctcctc
     3421 gtccgccact tcgtcaaaca caccgcccgc gaacagcagc acgacgagaa ggccatcgac
     3481 ccgcaagcca tgaccgcctt cctcaactac acatggcccg gcaacgtccg cgaactccag
     3541 aacgccgtcg agcgcgcctt caccttgagc ggcgagctga tcgacctcga cagcctcccg
     3601 ccccgcgtcc gcgacgccgc cgccaaagcc ggccccgacg ccatgcgcga ccccgacggt
     3661 ctgcgaccca ccctctccga aatcgaacgc cgtcacatcc tcgacaccct cgcctccgtc
     3721 aaccaggaca aggcccgcgc cgccaacatc ctcggcatcg acctctccac cctctaccgc
     3781 aagctcaaac gctacgacgc cgtgtaagcg cgaacgcggg gccgggggtc tgccccgcgc
     3841 gacgcgcggg tttgcgcgag cgcgagcgcg aactttatag tgtagggacc gatgtggaga
     3901 tacttacgag aggcgttgaa cgcggctctg atcgcgctgg ggattctgtc ggcggcgatg
     3961 gggctgaagg ggttcctgtt gtcgagccgt ttcatcgacg gcggggtgac gggcgtctcg
     4021 atgctgctcg cggacgtgac gggggtgccg ctggcggtgc tcatcccggt catcaacctg
     4081 ccgttcatcg cgctcggcta caggcagatc ggcaaaaagt tcgcgatcaa gagcgcgctg
     4141 gcgatcgcgg gcctcgcgct gtgcctcgcc ttcgtgaagt tcccggacgt gacgccggac
     4201 aagctgctga cggcggtgtt cggcggcttc ttcatcggcg cggggatcgg gctggcgatt
     4261 cgcggcgggt cggtgctgga cgggacggag gtggcggcgc tgctcgtgag caaggggagt
     4321 tccctgctga aggtcggcga cgtgatcctc gtgctgaaca tcttcatctt ctcggcggcg
     4381 gcgttcctgc tcggcgtcga gtcggcgatg tactcgatcc tcacctactt cgcggcgtcg
     4441 aagaccatcg acttcctgat ccacggcatc gaggagtaca ccgccgtcat catcatctcg
     4501 gaacggagcg acgaaatccg gcgggccatc acggacgacc tcaaccgcgg cgtgaccgtc
     4561 tacaaggggc ggggcgggat gaccggcgcc gagcaggaca tcctctactg cgtggtgacg
     4621 cggctggaga tcgggagggt caggagcgtg gcgcacggga tcgacggcgc ggccttcgtc
     4681 gtcgtccacc cgctctcgga cgtggacggc ggcgtcatca aacgccccgc gctgcactga
     4741 ccgggcggcg cccttcagtg cgcgcgcagc gctcgcgcga cgaggccgat gccgcccgcg
     4801 agcaggacga acatcaagat ggtgttgaaa aggctggtcg gctggaagta gatgtcgagg
     4861 ttcatcagga cgcccgtgag gatgatgacc gcgcccgcga agacgagcag ccagcccgcc
     4921 ttcgacctcc cgttgaagaa cagcaggccg atgccgaaga tgaggggcag cagcgagagg
     4981 ccgaagccgt tgaagcccca gaccctccag aacccgcccg tcaccgtcac gcgactggtc
     5041 aggatgtaag cccccgcgac ggccatcgcg aggccgagga gaaattcgcc gacgccgccc
     5101 ggcgtgccgc ccgcgccccg ccgacgttct tcgtcgcctt ctccagcgcc gcgccagctc
     5161 atcggcgctt attccttgcc gccgccgccg agcatgccgc cgaggccgcc gagcacgtcg
     5221 ccggccttgt cggtgaggcc gccgacggcg cccgcgccgc cggagatgac gccgtcgagc
     5281 tgcccggcga tgggcgcggg cagccgctcc ttcaggaacc cgagcacggt cgtcaccgcc
     5341 gtgcgcgcct gctcctccgc gatgcccgtc ttctgcgcga cctgtctgac aagttcatcc
     5401 atctctggtt cgcctcctcg gctgctgttg ggaaagcgtc gggtgaaact cgcggcggcc
     5461 cgcgccgcgg gtcagatgaa gaccaccgac tggaagttcg ggatgtcgaa gcgcttgcgg
     5521 cagcgcccgc agacgagcat gtcgtccatc cgcaccatgt agtcgcgggc gttgcggaac
     5581 tgcgcgcgct gctgctcgtt catgccgcgc ggcggctgcg ccttcttcgt gcgccgcagc
     5641 cagcgcacct gatactcgcc gcgctcgcgg cagtgcgggc aactataggt cgcgcgcttc
     5701 gtctcctgtc gttcgtcgaa gaagtctctc tcgttcataa ctcgtggggc taggggctag
     5761 gggctagagg ctagtcgaaa tcaaccggac ttcatcaacg tcctgcccga gacgcgaagc
     5821 gcggcgccga accatcatca ccagcctcta gtctccagcc cctggcctct ctttctttct
     5881 gtggtgtaaa acgatgagca ggccgacgag gccgttcagg agcatcaccg cgttggtgac
     5941 gacgaagacc cagttatcga ccagccaact gtagacggtg aagccgagcg aggccgccat
     6001 ctgcccgacg aagagccatt tcgaaacgcc ctcgctcgaa ccctcctgcc actgcttgta
     6061 gacctgtttg gcgatggtca gcaccaggat taccgagctg acccagccga ccgcctcgac
     6121 catccgccgc cctcttgttc ttccttttat cccgcgcgcc attatcgccg aaaccgcggc
     6181 ctcacgccaa aagccgccgc accgtttcga gcagcgtcac ggggtcgagc ggcttcggct
     6241 cgtaggcgtc ggcgccctgc gcgagcgtga gcgtcgcgct ccccgtcccg aagccggtcg
     6301 tgacgaggat cggcgtcgcg gcgatctcgg gcgtgtcgcg cacgcgccgc acgaggtgga
     6361 tgccgtccgc cgcgggcatc cacacgtccg tgatgatgag gtcgggccgc ccctcgaccg
     6421 cctgctcgta accgcgcatg ccgtcctccg ccaccgtgac cgcgtagccg cggcgcgcca
     6481 acgtcatccg caccagctcg cgcgacacct cgtcgtcttc cacaaccaga atctttttca
     6541 tttcggtaaa tggtgaatgg tgaatggtga atggtgtcga gcggctgcgg cagtcgcgcg
     6601 gttgattgca cgccttgaaa gaagcccgct ttttctattt accatttacc attcgctatt
     6661 cactccccct cgcatatctc gcgcaggtag cggaagctgt agatgcccgt gtcgtggccg
     6721 tcgctccagc ggaaggtgag cgcgtagcga ccgaccaact cgacgttccc gatcttcagc
     6781 tcgtcggcga ccgactcggg cttgagcacg cgctcgccgg tgaactcgtt gacgcactgc
     6841 gcgcacgggc acaggcggcg cagccgcggc gcctcgtaga cgcacgcgcg cccgtcgccc
     6901 cacgtgacgc gcagacgcgc gtccgtctcc tgcatgatct cgcgcggctc gacggcgggg
     6961 ccgcgcctca gcacttcact cataagacag ggccggaggt cggaggtcgg aggtcagaag
     7021 aaggaatcgc gcccgcaatg tttattccga cctccgacct ccggcctctc gtgcgttctc
     7081 tcgcaccgcc tgttcgacgg cggcggc
//
LOCUS       DBNGOH010001051         1586 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_159721, whole genome shotgun sequence.
ACCESSION   DBNGOH010001051 DBNGOH010000000
VERSION     DBNGOH010001051.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1586)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1586)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1586
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_159721"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(80..541)
                     /locus_tag="AC159P_20465"
     CDS             complement(80..541)
                     /locus_tag="AC159P_20465"
                     /inference="COORDINATES: protein motif:HMM:NF016246.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HIR3697874.1"
                     /translation="MSSTVTIFTIGHGRAAFADFLALLKQYGIEVVCDVRSAARSRWP
                     QYNGRVLEELLRENGVGYEWLPECGGKVVAPAEDLNRGLERIMELAADARVALMCSES
                     RPLTSHTQPRANCHRVGLLAPPLRARGARLVHILPEGDALEIDEATLPSIW"
     gene            complement(641..1225)
                     /locus_tag="AC159P_20470"
     CDS             complement(641..1225)
                     /locus_tag="AC159P_20470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HIR3697875.1"
                     /translation="MGVVSPADMKLRAAGSKYARVERERRYLLRELPPGLKLSDPHTQ
                     ITDNYITGTRLRLRKIRIPQTNEWTLKLTQKHAPAPAPPDFSRTLITNIYLNAYEYEV
                     LSVFEGNELRKNRYPYEHEGRKYSVDVFLGDLRGLVLAETDFATDEELDAFAPPAFAF
                     MDVTRDETFTGARLVELTADELRRELKSRVLRTE"
     gene            complement(1290..>1586)
                     /locus_tag="AC159P_20475"
     CDS             complement(1290..>1586)
                     /locus_tag="AC159P_20475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HIR3697876.1"
                     /translation="SQKIKEYARARGYIELFEKAGAELIDPSCGACINAGPGASKSPD
                     QVTVSAQNRNFPGRSGPGKVYLASPYVVAASAIAGHIVEPGEFLKEPLAELVEV"
BASE COUNT          250 a          479 c          556 g          301 t
ORIGIN      
        1 ccggaggtca gaggtcggag gtcagtaaag gcactgctgt ttcgactgac ctccgacctc
       61 tgacctccgg cctctgtctt taccagatgg aggggagcgt cgcctcgtcg atttcgaggg
      121 cgtcgccttc ggggaggatg tggacgaggc gtgcgccgcg cgcgcgcaag gggggcgcga
      181 gcaggccgac gcggtggcag ttggcgcgcg gctgcgtgtg gctggtgagg ggccgcgact
      241 ccgagcacat cagcgcgacg cgcgcgtccg ccgccagctc catgatgcgc tcaagcccgc
      301 ggttcaaatc ctccgcgggc gcgacgacct tcccgccgca ctcgggcagc cactcgtagc
      361 cgacgccgtt ctccctcaaa agctcttcca gcacgcgacc gttgtactgc ggccaacgcg
      421 aacgcgccgc gctcctcacg tcgcacacga cctcgatccc gtactgcttc agcagcgcga
      481 ggaagtccgc gaacgccgcg cgcccgtggc cgatggtgaa tatggtcacc gtggaagaca
      541 tgattaaaat agtgctgagg actgaggact gagtgagagc aagcgacgtg cgacggttga
      601 cgagagggat gctgtatgga ctcgtcatgg ttcatgtctt tcactcagtc ctcagcactc
      661 tgcttttcag ttcccggcgc agctcgtccg cggtcagctc gacgaggcgt gcgccggtga
      721 aggtctcgtc gcgcgttacg tccatgaagg cgaaggcggg cggggcgaag gcgtccagct
      781 cttcgtccgt ggcgaagtct gtttcggcga ggacgaggcc gcgcaggtcg ccgaggaaaa
      841 catcgacgga atacttgcgg ccttcgtgct cgtaggggta gcggttcttg cgcagctcgt
      901 tgccctcgaa gaccgagagc acttcgtact cgtaagcgtt gaggtagatg ttggtgatga
      961 gcgtgcgcga gaagtcggga ggcgcgggcg cgggcgcgtg cttctgcgtg agcttgagcg
     1021 tccactcgtt ggtctgaggg atgcgtattt tgcgcagccg caggcgcgtg cccgtgatgt
     1081 agttgtccgt gatctgcgtg tgcgggtcgg agagtttgag gcccggcggc agctcgcgca
     1141 aaagatagcg gcgctcgcgc tcgacgcgcg cgtacttgct gccggcggcc cgaagtttca
     1201 tgtccgcggg ggagacgacg cccatctttg tttcttccgc tcgaagttcg gtgtcggagg
     1261 cgaacgtgtt ccggcgcgtg gagcgcgcgt cagacttcca cgagttcggc caacggctct
     1321 ttcaggaact ctcccggctc gacgatgtgg cccgcgatgg ccgaggcggc gacgacgtaa
     1381 gggctggcga gatagacctt gccggggccg ctcctgccgg ggaagttgcg gttctgcgcg
     1441 ctgaccgtga cctgatccgg cgacttcgaa gcgcccggcc cggcgttgat gcacgcgccg
     1501 cacgaggggt cgatcaattc cgcgccggcc ttttcgaaaa gctcgatgta accgcgcgcg
     1561 cgcgcgtact ccttgatctt ctgcga
//
LOCUS       DBNGOH010001052         5014 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3275991, whole genome shotgun sequence.
ACCESSION   DBNGOH010001052 DBNGOH010000000
VERSION     DBNGOH010001052.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5014)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5014)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5014
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3275991"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..631
                     /locus_tag="AC159P_20480"
     CDS             <1..631
                     /locus_tag="AC159P_20480"
                     /inference="COORDINATES: protein motif:HMM:NF024816.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3697877.1"
                     /translation="DLAAELEAEVPVQPFAPAPGVSREEAPPPVSATTDADAAPPSAA
                     PRAGGLDPGLAAVFDEFREAVEDPEDEAPVQADYETHYNMGLAYREMGLHDQAIEELQ
                     TAVDLTAPGDGTARYLNCCNMLGHCFMEKGMPRPAAMWFRKGTEAPGHTEDEYQALRY
                     ELGAAYEQLGDLQRAIEVFSEVYAIDVSYRGVAGKLRELEERKAVNREP"
     gene            727..1056
                     /locus_tag="AC159P_20485"
     CDS             727..1056
                     /locus_tag="AC159P_20485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697878.1"
                     /translation="MASKLTVIFYIILCLEAGVVLTLVPWMPHWFGMADWGDNFLLVY
                     ASQTTGIEGLRAAVSSGWVRGAVTGLGLLNLAMAGWEIAHFGQTVRRLQGLDAKTRDE
                     GGRMKDE"
     gene            1119..1796
                     /locus_tag="AC159P_20490"
     CDS             1119..1796
                     /locus_tag="AC159P_20490"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF014622.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009229 - thiamine diphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HIR3697879.1"
                     /translation="MSRELPTPITCLVTGGETTPASEEFRRVLALVEAAVAARVSVVQ
                     LREKSLPARTLFELAARAAALTRGSETLLVVNDRADIARAAACDGVHLTAQSLEARTV
                     RRAFGEDFLIGVSAHTLAEARAARDGGADFAVLGPVFDTPSKRAYGPPLGTETFREAA
                     LALAPFPLLAIGGVARDNFRDVLLAGARGVAAIRLFGDATQLQRTVREFEDVFNEGAG
                     AARDSAA"
     gene            2002..2847
                     /gene="thiD"
                     /locus_tag="AC159P_20495"
     CDS             2002..2847
                     /gene="thiD"
                     /locus_tag="AC159P_20495"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019012582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HIR3697880.1"
                     /translation="MVEEEDRQPPPPVALTIAGFDPSGGAGVVADVKTFTAFGCFATA
                     AVTSLTYQNTTGVFGAAHQRAEAVRAQVLPVVEDFRVAGLKTGMLPSREVIEEVARLL
                     GETNLPAPVVDPVVRSTSGYDLIDDAALDALKKSLLPLARLVTPNVPEAERITGLSIR
                     DEEDMLGAARAMREMGARAVLVKGGHLTGRVALDILDDGGTVNTFAEARIETTATHGT
                     GCTLSAAIAACLALGLSLEEAVGAAKRFVTDAIRRAPGLGRGHGPVNHSVPARLRAQG
                     ANAEK"
     gene            2851..4155
                     /locus_tag="AC159P_20500"
     CDS             2851..4155
                     /locus_tag="AC159P_20500"
                     /inference="COORDINATES: protein motif:HMM:NF024206.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HIR3697881.1"
                     /translation="MKKSNPVYGIRAARTLSAIALLACVAAAGAAAFAQGDLTGGVGQ
                     FIAAQPKKEKVVKRAARKFEKPKAAPGVPKPKETAEDKLMEAASAGNAAAVEDLVRRG
                     ANVNAKSKDGCTPLIYAIAGGHAALAQSLVSKNADVNARCADGVTALMVAAETGDASL
                     VEALAGRGAAPGAKTEDGRTPLIFAASNGHLAAVRVLLAKGADASAAFRDGRNALVFA
                     AANGYAAIVEELHARKADVNARPEDGWTALMWASWFGHADVVKTLLDRGADVKARDRD
                     NDTALMKAATQGHLAVVQMLLARGAEADARGNGGGTALLFASSRGYYEIVEALLAKGV
                     DVNARNSVGLTPVITAAAYGRVPTLEVLLAKGANVNARTDKGFTALIAAANKGLAQTV
                     TLLIERGADVNAKYEDGRTALAFARENGQDKVVEVLKGAGAQ"
     gene            complement(4272..4760)
                     /locus_tag="AC159P_20505"
     CDS             complement(4272..4760)
                     /locus_tag="AC159P_20505"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIR3697882.1"
                     /translation="MANRIAKIETNMGTIRFELLEDESPKTAENFRLLAEKGFYDGVI
                     FHRVIKGFMIQGGDPTGTGRGGASAWGGRFDDEINRSSSLYQGPYAKGTVAMANAGPN
                     TNGSQFFIMHADYPLPPSYTKFGRVVEGQEVVDQIASVQRNRDDRPLQDVKMEKVTIE
                     EG"
     gene            complement(4841..>5014)
                     /locus_tag="AC159P_20510"
     CDS             complement(4841..>5014)
                     /locus_tag="AC159P_20510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HIR3697883.1"
                     /translation="DLEMLHYAGTGVVMGNAEASLRAVEKFHLTATNDEDGVAVAIEK
                     FVLNAERGMMNDE"
BASE COUNT          835 a         1731 c         1663 g          785 t
ORIGIN      
        1 cgacctcgcc gccgagcttg aggccgaagt ccccgtgcag cccttcgcgc ccgcgccggg
       61 cgtgtcgcgg gaagaggcgc cgccgccggt ctcggccacg acggacgcgg acgcggcccc
      121 tccgtccgcg gcgccgcgcg cgggcggact cgaccccgga ctggccgccg tcttcgacga
      181 gttccgcgag gcggtcgaag accccgaaga cgaagcgccg gtgcaggccg actacgagac
      241 gcactacaac atgggactgg cctaccgcga gatgggcctg cacgatcagg ccatcgagga
      301 gctacagacc gccgtcgatc tgactgcgcc cggcgacggc acggcgcgct acctcaactg
      361 ctgcaacatg ctcggccact gcttcatgga gaagggcatg ccgcgccccg ccgcgatgtg
      421 gttcaggaag ggcaccgagg cgcccggcca caccgaggac gaataccagg cgctgcgcta
      481 cgagctgggc gccgcctacg agcaactcgg cgacctccag cgcgccatcg aagtcttctc
      541 cgaagtctac gccatcgacg tttcctaccg cggcgtcgcc ggcaaactgc gcgagctgga
      601 agagagaaaa gccgtgaacc gtgaaccgta aaccgtgatc agtgaaaaga cggtgctcgc
      661 ttttcacggt ttacggttcg cggtctttga cggttcacgg tttacggttt acgattcacg
      721 gttcttatgg catcgaagct gaccgtcatc ttctacatca tcctgtgcct cgaagcgggc
      781 gtggtgctga cgctcgtgcc gtggatgccg cactggttcg ggatggcgga ctggggcgac
      841 aacttcctgc tcgtctacgc ctcgcagacc accggcatcg agggcctgcg cgccgccgtc
      901 tcctcgggct gggtgcgcgg cgcggtgacg ggcctcggcc tcttgaacct ggcgatggcc
      961 ggctgggaga tcgcgcactt cggccagacc gtccgccgcc ttcagggcct cgacgccaaa
     1021 accagggatg aaggcggaag gatgaaggat gaataaagaa cactcaaccg acgttgagca
     1081 gttccttctt catccttccg ccttcatcct ttcccgcgat gtcccgtgaa ctcccgaccc
     1141 cgatcacctg cctcgtcacg ggcggcgaga cgacgcccgc gagcgaggag ttccgccgcg
     1201 tcctcgcgct cgtcgaagcg gcggtcgccg cgcgcgtctc cgtcgtccaa ctgcgcgaga
     1261 agagtctccc tgcgcgcacg ctcttcgagc tggccgcgcg cgccgcggcg ctgacgcgcg
     1321 ggagcgagac cctcctcgtc gtcaacgacc gcgccgacat cgcccgtgcc gccgcctgcg
     1381 acggcgtcca cctcaccgcg caatcgctcg aagcgcgaac tgtccgccgc gcgttcggcg
     1441 aagactttct catcggcgtc tcggcgcaca cgctcgcgga ggcgcgcgcg gcgcgcgacg
     1501 gcggcgcgga cttcgccgtc ctcggccccg tcttcgacac gccctcgaaa cgcgcctacg
     1561 gcccgccgct cggcaccgag actttccgcg aagccgcgct cgccctcgcg cccttcccgc
     1621 tccttgccat cggcggcgtc gcaagagata acttccgcga cgttctcctt gcgggcgcgc
     1681 gcggcgtcgc ggccatccgc ctcttcggcg acgcaacgca actgcaacga accgtgcgcg
     1741 agttcgagga cgtatttaac gagggtgccg gggcggcgcg tgattccgcc gcgtagcggc
     1801 ggctgagttt agcccggcgt tttaacgccg ggtaatcacg tcgtcaagat tcttcgtcgc
     1861 gtcagcgacg agtgaatttc tatcaagcgt cgctgacgcg acgcggttcg gcgcgctcgc
     1921 ccctatcccg gctctgaaga gccgggctaa actcaaccgc cgctacgcgg cgtgttgatg
     1981 cgctccgcaa ttcaccgtga gatggttgaa gaagaagaca gacaaccgcc gccgcccgtc
     2041 gcgctgacca tcgcgggctt cgacccctcg ggcggggcgg gcgtagtcgc cgacgtgaag
     2101 acgttcaccg ccttcggctg cttcgcgacg gctgccgtca cgagcctgac ctaccagaac
     2161 acgacgggcg tcttcggcgc ggcgcaccaa cgggcagaag ccgtgcgcgc gcaagtcctg
     2221 ccggtcgtcg aagacttccg cgtggcgggc ttgaagacgg ggatgctccc ttcgcgcgag
     2281 gtgatcgaag aggtcgcgcg gctgctcggc gagacgaatt tgccggcgcc ggtggtcgat
     2341 ccggtcgtgc gctcgacctc cggttacgat ttgatcgacg acgcggcgct cgacgccttg
     2401 aagaaatccc tgctgccgct cgcgcgcctc gtcacgccca acgtgcccga ggccgagcgc
     2461 atcacgggtc tctcgatcag ggacgaagaa gacatgctgg gcgccgcgcg cgcgatgcgc
     2521 gagatgggcg cgcgcgccgt gctcgtgaag ggcggccacc tgacggggcg ggtcgcgctc
     2581 gatatattgg acgacggcgg gacggtcaac accttcgccg aagcccgcat cgaaaccacc
     2641 gcgacgcacg gcacgggctg cacgctctct gccgccatcg ccgcgtgcct cgcgctcggc
     2701 ctgagcctcg aagaagcggt cggcgcggcc aagcgcttcg tcaccgacgc catccgccgc
     2761 gcgcccggtc tgggccgcgg ccacggcccc gtcaaccaca gcgtccccgc gcgcctccgc
     2821 gcgcaagggg cgaacgcgga gaagtaacag atgaagaagt cgaaccccgt ttacggcatc
     2881 cgggccgcgc gaacgttgtc ggccatcgcg ctccttgcgt gcgtcgccgc cgccggcgcc
     2941 gccgcgttcg cgcagggcga tctgacgggc ggcgtcgggc agttcatcgc cgcgcagccg
     3001 aagaaggaga aggtggtcaa gcgggccgcc cgcaagttcg agaagccgaa ggcggcgccc
     3061 ggcgtgccga agccgaaaga gaccgccgag gacaagctga tggaggccgc gagcgcgggc
     3121 aacgccgcgg cggtcgaaga cctcgtgcgg cgcggcgcga acgtgaacgc gaagagtaag
     3181 gacggctgca cgccgctgat ctacgcgatc gcgggcgggc acgcggcgct cgcgcagagt
     3241 ctggtctcga agaacgcgga cgtgaacgcg cgctgcgccg acggcgtgac ggcgctgatg
     3301 gtcgcggcgg agacgggcga cgcctctctg gtcgaagccc tcgcgggccg cggcgccgcg
     3361 ccgggcgcga agacagagga cgggcgcacg ccgctcatct tcgccgcgtc gaacggccac
     3421 ctcgcggccg tgcgcgtgct gctggcgaag ggtgcggacg cgtccgccgc tttcagggac
     3481 gggcgcaacg cgctggtctt cgcggcggcc aacggctacg cggccatcgt cgaagagctg
     3541 cacgcgcgca aggcggacgt gaacgcgcgc cccgaagacg gttggacggc gctgatgtgg
     3601 gcctcctggt tcggccacgc ggacgtggtc aagactctgc tcgaccgggg cgcggacgtg
     3661 aaggcgcgcg acagggacaa cgacacggcc ctgatgaagg cggcaacgca ggggcacctc
     3721 gccgtcgtgc agatgcttct ggcgcgcggc gccgaggcgg acgcgcgcgg caacggcggc
     3781 ggcaccgcgc tcctgttcgc ctcgtccaga ggctactacg agatcgtcga ggcgctgctc
     3841 gccaagggcg tggacgtgaa cgcgcgcaac agcgtggggc tgacgcccgt catcaccgcc
     3901 gccgcctacg ggcgcgtgcc cacgctcgaa gtgctcctcg ccaaaggcgc caacgtcaac
     3961 gcccgcaccg acaagggctt caccgcgctc atcgccgccg ccaacaaggg tctcgcgcag
     4021 accgtcacgc tcctcatcga gcgcggcgcc gacgtgaacg ccaagtacga agacgggcgc
     4081 accgccctcg ccttcgcacg cgagaacggc caggacaagg tcgtcgaggt gctgaagggc
     4141 gcgggagcgc aatagagccg taaatggtaa atggtaaatg gtgaatagag ggttgcgccc
     4201 tgccccgcca gagttttggc attcaaggca cgcgacctct caaccaccat ttaccattta
     4261 ctatttacca ttcacccttc ttctatcgtc accttctcca tcttcacgtc ttgcagcggt
     4321 cggtcgtcgc ggttgcgttg gacggaggcg atctgatcga ccacctcctg cccttcgacg
     4381 acgcgcccga acttggtgta gctcggcggg agcgggtaat cggcgtgcat gatgaagaac
     4441 tgcgagccgt tggtgttcgg cccggcgttg gccatcgcca cggtgccctt cgcgtacggc
     4501 ccttgataga gagatgacga gcggttgatc tcgtcgtcga agcgcccgcc ccaggccgac
     4561 gccccgccgc gccccgtccc cgtggggtcg cccccctgaa tcatgaagcc cttgatgacg
     4621 cggtggaaga tgacgccgtc gtagaagcct ttctcggcca gcagccggaa gttctccgcc
     4681 gtcttcggcg actcgtcctc caacagctcg aagcggatcg tgcccatgtt cgtctcgatc
     4741 ttcgcgatgc ggtttgccat agctcctcct gcaaatgatg aatgatgaac gcggaatgat
     4801 gaagaagatt cagctcgaag gatgagaggc accgtgcttc tcattcatca ttcatcattc
     4861 cgcgttcagc gtttagaaca aacttttcga tggcgacggc gacgccgtcc tcgtcgttgg
     4921 tcgccgtcag gtgaaacttc tcaaccgcgc gcagtgacgc ttcggcgttg cccatcacga
     4981 cgccggtgcc ggcgtagtgc agcatctcaa gatc
//
LOCUS       DBNGOH010001053         2528 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1443485, whole genome shotgun sequence.
ACCESSION   DBNGOH010001053 DBNGOH010000000
VERSION     DBNGOH010001053.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2528)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2528)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2528
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1443485"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..528)
                     /locus_tag="AC159P_20515"
     CDS             complement(<1..528)
                     /locus_tag="AC159P_20515"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: protein motif:HMM:NF014494.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0106141 - flavin prenyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HIR3697884.1"
                     /translation="MELTVAITGASGTIYAHRTLLHMAASGVVERINLVMSNTAPVVA
                     RVEMGASLGDGDAKSVNEWLGLPAKSKLVRVHRLDNLAAAPASGSHPQAGMVVVPCSM
                     GTLGAVASGAGSSLIHRAADVTLKEGRKLVLVVRESPYNAIHLENMLRLAHAGARILP
                     ASPGFYHRPRTIEELV"
     gene            complement(552..818)
                     /locus_tag="AC159P_20520"
     CDS             complement(552..818)
                     /locus_tag="AC159P_20520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697885.1"
                     /translation="MKVSNERREPLTVWLEPWGEDYTLLPDETFDVVAEGASPDFHFD
                     MACKETDVLVYAEGDCTCVFVLRGGEALECGHNRERSVSPVQWD"
     gene            977..1738
                     /gene="larB"
                     /locus_tag="AC159P_20525"
     CDS             977..1738
                     /gene="larB"
                     /locus_tag="AC159P_20525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008516591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:7770034 - nickel-pincer nucleotide cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HIR3697886.1"
                     /translation="MDLREIERLLRAFKSGELGEREAARAIGELGFEDIGHTRVDHAR
                     AARQGFPEVVFGAGKTRAQVVEIVERLVRRAPNVLVTRTDEGTYGEVRNVCTEAEWHP
                     AARMIRVMRDREERGAGTVMVVTAGTSDIPVAEEAALTAEAMGNRVERVWDAGVAGVH
                     RIFAERERLRRARVVIVAAGMEGALPSVVGGLVAAPVIAVPTSIGYGASFGGVAALLG
                     MLNSCSSNVTVVNIDNGFGAGFVAALINRKQSDEG"
     gene            1835..>2528
                     /locus_tag="AC159P_20530"
     CDS             1835..>2528
                     /locus_tag="AC159P_20530"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014503.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase/lipase family protein"
                     /protein_id="HIR3697887.1"
                     /translation="MTSCGARRTPDLARIFAGAKAREGKRPVIVIPGVLGSQIVNRRT
                     GEVVWPSAFRSSDDGLNLPVTPDLASNRDDLVASKIIETAKLARLAPEVYVYRALLWA
                     LENFAGYREGEWDAPPADGDRDTFYVFPYDWRRDNVETAQEFVRRVEELKRKLGRPDL
                     RFNVVAHSMGGLVARYAAMYGDEDLPPEGTRPVPTWEGAAHVNKIFMFGVPNEGSAEA
                     FTTLTEGYSVTEG"
BASE COUNT          394 a          857 c          880 g          397 t
ORIGIN      
        1 caccagctct tcgatcgtcc gcgggcggtg gtagaagccg ggactcgcgg gcaggatacg
       61 cgcgcccgcg tgcgcgaggc ggagcatgtt ctcaaggtgg atcgcgttgt acggcgactc
      121 gcgcacgacg aggacgagct tgcgcccctc cttcagcgtc acgtccgccg cgcggtggat
      181 gaggctgctg ccggcgcccg aggcgaccgc gccgagcgtg cccatcgagc acggcacgac
      241 gaccatcccc gcctgcgggt gcgaccccga agcgggcgcc gccgcgaggt tgtccagacg
      301 gtgcacgcgc acgagcttcg acttcgcggg caggcccagc cactcgttga ccgacttcgc
      361 atcgccgtcg ccgaggctcg cgcccatctc gacgcgcgcg accacgggcg ccgtgttcga
      421 catcacgagg ttgatgcgct cgaccacccc gctcgccgcc atgtgcagca gcgtgcggtg
      481 cgcgtagatc gtgccggagg ccccggtgat ggcgacggtc agttccatag cactcgcgga
      541 aacagtcaga gctaatccca ttgcaccggc gagacagacc tctcgcggtt gtggccgcat
      601 tctaaagcct cgccgcctcg caacacgaac acgcacgtgc agtcgccttc cgcgtagacc
      661 agaacgtctg tctctttgca ggccatgtcg aagtggaagt caggcgacgc gccttcggcc
      721 accacgtcga aagtctcgtc gggcagcagc gtgtagtctt cgccccacgg ctcaagccac
      781 acggtcagcg gctcgcgtcg ctcgttactg actttcatgc tgtgaacgct gttccccacg
      841 cccaaccctt tcaagcggtg tccgaatctt acgggttaga caggcgaggg gcaagcaggc
      901 cacgtttcgg gcgcgcgcgg gttgggtgct agaatgcccg ctcgattcga cagggcaagg
      961 gctttgatct caactcatgg atttacgcga aattgagcgg ctgctgcgcg ccttcaagag
     1021 cggcgagctt ggagagcggg aggcggcgcg cgccatcggc gagctggggt tcgaggacat
     1081 cggccacacg cgcgtcgatc acgcgcgcgc cgcgcggcag ggctttccgg aggtcgtctt
     1141 cggcgcgggc aagacgcgcg cgcaggtcgt ggagatcgtc gagcggctcg tgcggcgcgc
     1201 gccgaacgtg ctcgtcacgc gcacggacga gggcacctac ggcgaggtgc gcaacgtctg
     1261 cacggaggcc gagtggcacc cggcggcgcg catgatccgc gtcatgcgcg accgcgagga
     1321 gcgcggcgcg ggcaccgtca tggtcgttac cgcgggcacg agtgacatcc cggtcgccga
     1381 agaggccgcg ctcaccgccg aggcgatggg caaccgcgtc gagcgcgtct gggacgcagg
     1441 ggtggcgggc gtccaccgca tcttcgccga gcgcgagcgg ctgcggcggg cgcgcgtcgt
     1501 catcgtcgcg gcggggatgg agggcgcgct cccttcggtc gtcggcgggc tggtcgccgc
     1561 gcccgtcatc gccgtgccga cgagcatcgg ctacggcgcg tccttcggcg gcgtcgccgc
     1621 gctcctcggg atgctcaact cctgctcctc gaacgtcacg gtcgtcaaca tcgacaacgg
     1681 cttcggcgcg ggcttcgtcg ccgcgctcat caaccgcaaa caaagtgatg agggctgagg
     1741 actgagtatg aattcaaaac tcaggaggcg ctcggcgtgc ctttccctca gtcctcagtc
     1801 ctcagtcctc atgactctcg tcatcgtgct aacgatgacg agctgtggcg cgcggcggac
     1861 gcccgacctg gcgcgcatct tcgcgggggc gaaggcgcgg gaggggaagc ggcccgtgat
     1921 cgtcatcccc ggcgtgctcg gctcgcagat cgtcaaccgg cggacgggcg aggtggtgtg
     1981 gccctcggcc ttccgctctt cggacgacgg gctgaatctg cccgtcacgc ccgacctcgc
     2041 ctcgaaccgc gacgacctcg tcgcctcgaa gatcatcgag acggcgaagc tggccaggct
     2101 cgccccggag gtctacgtct accgcgcgct gttgtgggct ttggagaact tcgcgggcta
     2161 ccgcgagggc gagtgggacg cgccgcccgc ggacggcgac cgcgacacgt tctacgtctt
     2221 cccctacgac tggcggcgcg acaacgtcga gacggcgcag gagttcgtcc ggcgcgtcga
     2281 agagctgaag cggaaactcg gccggcccga cctgcgtttc aacgtcgtcg cgcactcgat
     2341 gggcgggctg gtcgcgcgct acgcggcgat gtacggcgac gaagacctgc cgcccgaggg
     2401 tacgcgcccc gtgccgactt gggagggcgc cgcgcacgtc aacaagattt tcatgttcgg
     2461 cgtgcctaac gaaggctccg ccgaggcgtt caccacgctg accgaaggct actccgtgac
     2521 cgagggcc
//
LOCUS       DBNGOH010001054         6007 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2543651, whole genome shotgun sequence.
ACCESSION   DBNGOH010001054 DBNGOH010000000
VERSION     DBNGOH010001054.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6007)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6007)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6007
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2543651"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..801
                     /locus_tag="AC159P_20535"
     CDS             <1..801
                     /locus_tag="AC159P_20535"
                     /inference="COORDINATES: protein motif:HMM:NF024934.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HIR3697888.1"
                     /translation="THRAGHGDAETPFGARRVMGAAPRTVTVRKNIGSSSLNALLANE
                     LLAALPAEEFERLLPHFEPVTLTAGEDLYQFEGGIHFAYFPESAVISQLHVLGDGNTI
                     EAAMVGREGFAGLSAVFNAPQPVYWTRVLIAGSALRIRVDILKQEFERGRVLQRVLLA
                     YAGARIAQLSQRAVCSGRHRVDERLCCWLLMLHDRAGEEQLQLTHELIASHLGVRRAG
                     ITGSATVLREMDIISYCRGLIRILDRRRLEAAACECYEMMVQPAARVL"
     gene            861..1517
                     /locus_tag="AC159P_20540"
     CDS             861..1517
                     /locus_tag="AC159P_20540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012409053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HIR3697889.1"
                     /translation="MPREEYERLAPHLVEVSLKLRQVLFSPGDEIAYAYFPTDSIVSL
                     LTDLSDGSGMEVGLVGREGLVGVSVILGGSETKVATVQAEGNALKIRAGALREEFRRG
                     GTLQNGLLRYTHALMTQISQSVVCNVRHPLPGRLARWLLMYHDRLGRDEFSLTHEFMA
                     AMLGVRRAGVSDVANQLQRSGFISYRRGHVRIVDRKGMEEFACECYPVVEEKFDDYLL
                     "
     gene            1615..1977
                     /locus_tag="AC159P_20545"
     CDS             1615..1977
                     /locus_tag="AC159P_20545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3697890.1"
                     /translation="MKIPDGQATTILIVEDIGWIRSSMIRSVERYGYRALTAEDDVEA
                     LALAEREPPEIILTEEELPTFGALMARLREHPVLHGVPVVIVNPDAEEGERYGGCILL
                     TDYNQISQVLNFFPESIP"
     gene            complement(2050..3000)
                     /locus_tag="AC159P_20550"
     CDS             complement(2050..3000)
                     /locus_tag="AC159P_20550"
                     /inference="COORDINATES: protein motif:HMM:NF012798.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HIR3697891.1"
                     /translation="MSASDGSAQPLRFDPRLAAPFCEKSLSEETRRAYRRVIIEFFLF
                     VKGTHPSEVTPAHVLAWRDALKGERKKAVTISFKLTVVRSFFEYLRAGGYVHLNPAST
                     KLVPPPAVSDEMKGRALTSKEVRYLLAGPDRSKPEGARDYALLLVMLRLGLRVSEACS
                     LRTSSVHWSHGRWTIRLKVKGGRERTLPLPQEVRVAMDEYVRLDRQRREALHSGGEDA
                     YLFQPVVNYRTLEFSKPLSTRMAWNVVRRWAEYGGVGELSPHDLRRTAITRALDQGLS
                     YRQVQMMSGHRDPKTVMRYDHGRENLEQNAVNFLDYDEEA"
     gene            3267..3428
                     /locus_tag="AC159P_20555"
     CDS             3267..3428
                     /locus_tag="AC159P_20555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697892.1"
                     /translation="MPTSYEIYQTYLRGPVAVLRLFEQSLGTQAIYGPPEQDIQQRTS
                     DALQTQASC"
     gene            3786..5060
                     /locus_tag="AC159P_20560"
     CDS             3786..5060
                     /locus_tag="AC159P_20560"
                     /inference="COORDINATES: protein motif:HMM:NF012862.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HIR3697893.1"
                     /translation="MLSTLVPAGRAQGPAQGGARPATGGAVSALPAAAKRFALVIGVD
                     RYDDTQITTLGGASNDARALASALTAHAGFPSDQVVLLASDQPAERRPTRGNVLRRLS
                     NLAEVVPKDGLLLFAFAGHGMERGGQAFLLPSDAQVSDDVTLLEQTAVNVAQIKGWVR
                     KTGVRQVLLLLDACRNDPAGRSDADNPLTETYTRALDLDRRNREVEAFVTLYATAIGR
                     RAYEFREKRQGYFTWALVEGLRGAAANDRGEVTLDRLISYLQERVPKQVALDLGRGKV
                     QRPYAVVEGYKADELVLAVADGRGLSIPETGVGDGDGPEAGGAEEAGGAEDKGRQAPH
                     VTKLVETAWEGLAAGGRYTVEFLPEGKLRYTLVDNKKTTGGSWKQTGDFIQMSVGGYS
                     ALSGRIEGDVIRGEGSNVAGEKWSWALTRKEK"
     gene            5478..>6007
                     /locus_tag="AC159P_20565"
     CDS             5478..>6007
                     /locus_tag="AC159P_20565"
                     /inference="COORDINATES: protein motif:HMM:NF014214.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M36 family metallopeptidase"
                     /protein_id="HIR3697894.1"
                     /translation="MTNLFVMHNRMHDWTYHLGFTEENWNAQQNNFGNAGAGGDPVLG
                     QSQAGAADGGYPAYLGRDNANMRTLPDGVPSITNMYLWQPIRGAFYAPCVDGDYDMAV
                     IGHEYGHMIENRMIGKGANRSGHHAGAMGESNGDLNAMEILNEYGFVPVAGENPYAVG
                     AYATGDKERGIRNYGM"
BASE COUNT         1062 a         1979 c         1949 g         1017 t
ORIGIN      
        1 actcaccgcg cagggcatgg cgacgccgag actcctttcg gcgcgaggcg ggtgatgggt
       61 gcggctccgc gcacggtcac tgtgcgaaag aacatcgggt cgtcctcgtt gaacgcactg
      121 ctcgccaacg aacttctggc cgccctgccc gccgaggagt tcgagcgcct cctcccgcat
      181 tttgaaccag tcaccctcac cgccggggaa gacctttacc aattcgaggg gggcattcat
      241 ttcgcctatt tcccggaatc agccgtcatc tctcaacttc atgtcctcgg ggacgggaac
      301 accatcgagg ccgcgatggt cggcagggag ggctttgccg gtctctccgc cgtcttcaac
      361 gcaccgcagc ccgtctattg gacgcgggtg ctcatcgcgg gcagcgccct gcggatcagg
      421 gtggacatcc tcaaacagga gttcgagcgc gggcgcgtgc tgcaaagggt gctcctcgct
      481 tacgcgggcg cgcgcatagc tcaactctcc cagagggccg tttgcagtgg ccgccataga
      541 gtggatgagc gtttgtgctg ttggctgctg atgctgcacg accgcgccgg cgaagagcag
      601 ttacaattga cgcacgaact catcgccagt cacctcggcg tgcggcgcgc gggtattact
      661 ggcagcgcca ccgtgctgcg ggagatggat attatctctt attgtcgcgg cctcatccgc
      721 atcctcgacc gtcgaaggct tgaggccgcc gcctgcgaat gctatgagat gatggttcag
      781 cctgccgcac gggtgcttta agctatgaac ggatgacagt cccgacgcaa acttcgactg
      841 aaaaccgcct gctttcagcc ttgccgcgcg aggagtacga gcggctcgcc ccgcacctcg
      901 tcgaggtctc tctgaagctc cgacaggtgc tcttcagccc cggtgatgag atcgcgtatg
      961 cctacttccc gaccgactca atcgtctccc tgctcacaga cctttctgat gggagcggca
     1021 tggaggtggg tctggtcggg cgcgagggcc tcgtcggcgt ctccgtcatc ctcggcggca
     1081 gcgagacgaa agtcgccacg gtacaggcgg aaggcaacgc gctaaagatc agagcgggcg
     1141 cgctccgcga agagttccgc cgcggcggca cgctgcaaaa cggactgctg cgctacacgc
     1201 acgcgctgat gacgcaaatc tcgcaatccg tcgtctgtaa cgtgcgccac cctctgcccg
     1261 gaaggctcgc ccgctggctc ctgatgtacc acgaccgtct ggggcgggac gaattttcac
     1321 tgacccacga gttcatggcc gcaatgctcg gcgtgcgccg cgccggcgtc agcgatgtcg
     1381 ccaaccagct gcaaaggtcg gggttcatca gttaccggcg cggccacgtc cgtatcgtcg
     1441 accggaaagg gatggaagag ttcgcctgcg agtgctaccc ggtggtcgaa gaaaaattcg
     1501 atgattacct gctctgacgg gtctgcccgg aaaggtacag actcgaaggc cctcctctgc
     1561 tgtacaatcg gacgccgtcg cgtaggcacg gcgtttctgt gggggcacac cttaatgaaa
     1621 atccctgacg ggcaggcgac gacgatcctg attgttgaag acatcggctg gattcgctcc
     1681 agcatgatac gttcggtcga gcgttatggc taccgcgcct tgaccgcgga ggacgacgtg
     1741 gaggcgctcg ccctcgccga gcgcgaaccg cccgagataa tcctgacgga agaggagttg
     1801 cccaccttcg gcgccctgat ggcgcgcctc cgcgagcacc ccgtcctcca cggcgtgccg
     1861 gtcgtgatcg tcaacccgga cgcggaagag ggcgagcggt acgggggctg catcctgctc
     1921 accgactaca accaaatcag ccaagtcttg aacttcttcc ccgagagtat tccgtgagat
     1981 tccgacccgg caccgggatt caatacccgc tggaaagctg tatcatgtag agaggctcat
     2041 ccctacgcac tacgcctctt catcgtagtc caagaagttg acggcgttct gctccaggtt
     2101 ctcccgcccg tggtcgtacc gcatcaccgt cttcggatcc ctgtgcccgc tcatcatctg
     2161 cacctgccgg tacgagaggc cctggtcgag tgcccttgtg atggccgtgc gcctcaagtc
     2221 gtgcggcgac agttcgccca ccccgccgta ctcggcccac cggcgcacca cgttccacgc
     2281 catcctcgtc gacaaaggct tcgagaactc cagcgtccgg tagttcacga cgggctggaa
     2341 caggtaggcg tcttccccgc ccgagtgcag cgcttctctc ctctgccgat ccaggcgaac
     2401 gtactcgtcc atcgcaaccc gcacctcctg cggcagcggg agcgtgcgct ccctcccgcc
     2461 cttgaccttg agacggatgg tccaccgccc gtggctccag tgcaccgaag aagtacgcaa
     2521 gctacaggcc tcggacactc tcaagccgag ccgcagcatc acgagcagca gcgcgtagtc
     2581 cctcgcgcct tccggcttcg agcggtcggg tcccgcgagc aggtagcgca cctcctttga
     2641 agtcagcgcc cggcccttca tctcgtcgga gacggcgggc ggcggcacca gtttggtcga
     2701 ggcggggttg agatggacgt accctccggc gcgcagatac tcgaagaagg agcgcaccac
     2761 cgtcagcttg aagctgatgg tgacggcttt ctttcgctcg cccttgagcg cgtcgcgcca
     2821 ggcgaggacg tgggcagggg tgacctcgga ggggtgggtg cctttgacga agaggaagaa
     2881 ctctatgatg acgcggcggt aggctcgtct ggtctcctcg gagagcgact tctcgcagaa
     2941 gggtgcggcc agacgggggt cgaagcgtag gggctgcgca gacccgtcgc tggcagacat
     3001 aggtgcaggt agatagtcag gcacgtggct tcagtgactc ctgtggcgcg tttcgacgac
     3061 gaatgcaaga ggaatgatgg aaaagtccgc ttcaccagtc aagcggtttc ttcttgaaat
     3121 accccttttg cgcatataac gctgccgggt gtctgttcag gaggtcgact aaactgcgga
     3181 aaaaagcgac ttcggcgaca ccctgaggcc cctcccgtcg gttataagcc gtcttctctc
     3241 gcgatgcctc ctccggaagg gcagtgatgc cgacctccta cgagatctac cagacgtacc
     3301 tgcgtggccc ggtggccgtc ctccgcctct tcgagcagtc tttggggaca caggccatct
     3361 acggcccgcc ggagcaggac atccagcagc gcaccagcga cgccctgcag acgcaagcgt
     3421 cgtgctgact aatgccccac aatcgctttc ccctacaagc catggacgtg tcgttcccgc
     3481 gcggtgcccc gtgccgccgg tcgcccgtga tccccgacgt ggccaattac cgcgttaccg
     3541 tacgggggct tggcgcggag tttgtccgtc caccgacaaa ctcgagggat cacggttaga
     3601 gggcagcagg tttgcttcgt ccccccgcgg ccttcaaccg aatccgactt gcgggctgcg
     3661 gctcggacgc gagcgcgggc cgccgaaccg accggcatgg cctgcgggcg gccggtccga
     3721 aaggagtcgc cttagtgaat cgccgttcgt tgactctctt cgccttcgcc gtttccacac
     3781 tcacgttgct gtccaccctt gtgcccgccg gtcgggcgca gggcccggcg cagggcggcg
     3841 cccgaccggc gactggcggg gccgtcagcg ccctgcccgc cgcggccaag cggttcgcgc
     3901 tcgtcatcgg cgtcgacagg tacgacgaca cgcagatcac cacgctcggc ggcgcctcga
     3961 acgacgcccg cgccctcgcc tcggcgctca ccgcccacgc cggcttcccc tcggaccagg
     4021 tcgtcctgct ggcctccgac cagcccgccg agcgccggcc cacgcgcggc aacgtcctgc
     4081 ggcgcctctc gaacctcgcc gaggtcgtgc ccaaggacgg gctgctgctg ttcgccttcg
     4141 ctggccacgg catggagcgc ggcgggcagg cgttcctgct cccttcggac gcgcaggtct
     4201 cggacgacgt gacgctgctc gagcagacgg ccgtcaacgt cgcgcagatc aagggttggg
     4261 tgcggaagac cggcgtcagg caggtcctgc tgctcctcga cgcctgccgc aacgatccgg
     4321 ccgggcgctc ggacgccgac aaccctctga ccgagaccta cacacgcgcc ctcgatttgg
     4381 accggcgcaa ccgcgaggtc gaggcgttcg tcacgctcta cgcgacggcc atcgggcggc
     4441 gcgcttacga gttccgcgag aagcggcaag gctatttcac ctgggcgctg gtcgaagggc
     4501 tgcggggtgc ggcggcgaac gaccggggcg aggtgacgct ggaccggctg atctcgtacc
     4561 tgcaggagcg cgtgcccaag caggtcgcgc tcgacctggg caggggtaag gtgcagcgcc
     4621 cgtacgccgt ggtcgaaggg tacaaggcgg acgaactggt gctggccgtc gccgacgggc
     4681 ggggcctctc catcccggag acgggtgtcg gggacgggga cgggccggag gcgggcgggg
     4741 ccgaagaggc gggaggcgcc gaggacaagg gccggcaggc gccacacgtc accaagctcg
     4801 tagagaccgc ctgggagggc ctcgcggcgg gcgggcgtta cacggtcgag ttcctgccgg
     4861 agggcaagct ccgctacact ctcgtggata acaagaagac gacgggcggg agttggaagc
     4921 agaccggcga cttcatccag atgtcggtcg gcggctactc ggcgctgagc ggcaggatcg
     4981 agggcgatgt catcaggggc gaagggtcga acgtcgccgg cgagaagtgg agctgggcgc
     5041 tgacgcggaa agagaaatga acggcgcccc gggccgcgtc ggcccggagc aggggggacg
     5101 gggcggggcg cgggcgtgcc ccgccccgtc ggcattctcg tgaccgcatg agcagcggcc
     5161 gggcgctgac tgcctagtcc gtccgcgccg tcgcgcagca tcaatcgacg ctcgcgccct
     5221 cacgcctcgc tttgaataga tcaccgcacc gcaagcgttc cggattttaa taaacgtctg
     5281 aaattacgca gcggcgctaa aggcgtctag ctgcggcttt gcctatttat tcggcaggct
     5341 gtgcataaaa gccacgaaca tgcagcatat caaccgatgt atgcgccgtt tgacgaataa
     5401 ataggcaaag ccggtacgcg agccgctgca acccgacgaa cttcgtcccc ggcgtctcga
     5461 acgacatctc ggctgctgtg acgaacctct tcgtgatgca caaccggatg cacgactgga
     5521 cgtaccacct cggcttcacc gaggagaact ggaacgcaca gcagaataac ttcggcaacg
     5581 cgggcgccgg gggagacccg gtcctcgggc agtcgcaggc gggcgcggcg gacggcggct
     5641 acccggccta cctcggtcgc gacaacgcga acatgcggac gctgcccgac ggcgtcccgt
     5701 cgatcaccaa catgtacctc tggcagccga tccgcggcgc cttctacgcg ccctgcgtcg
     5761 acggcgacta cgacatggcg gtgatcggcc acgagtacgg ccacatgatc gagaaccgca
     5821 tgatcggcaa gggcgccaac cggagcggtc accacgccgg cgccatgggc gagtcgaacg
     5881 gcgacctcaa cgcgatggag attctgaacg agtacgggtt cgtccccgtc gcgggcgaga
     5941 atccgtacgc cgtcggcgcc tacgcgaccg gcgacaagga gcggggcatc cgcaactacg
     6001 ggatgaa
//
LOCUS       DBNGOH010001055         2424 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1443959, whole genome shotgun sequence.
ACCESSION   DBNGOH010001055 DBNGOH010000000
VERSION     DBNGOH010001055.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2424)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2424)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2424
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1443959"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="AC159P_20570"
     CDS             <1..199
                     /locus_tag="AC159P_20570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697895.1"
                     /translation="MYELNRPLIAGYIQAMRVMQQEFGLAGEPDINVLARLPGALQAS
                     REGVGPEVYAGVELALVGALD"
     gene            245..664
                     /locus_tag="AC159P_20575"
     CDS             245..664
                     /locus_tag="AC159P_20575"
                     /inference="COORDINATES: protein motif:HMM:NF019940.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease YicC domain-containing protein"
                     /protein_id="HIR3697896.1"
                     /translation="MRTRLDEIEHHVPIIESVAGGQLDTYRARVYKRITELLARDGLE
                     IVELDQGRLAQEVAYLADRSDVTEELARLKSHITQFRETIDAGGEAGKRLDFLLQELN
                     REANTVLSKSTDLAIKESALAIKAAVEKLREQVQNVE"
     gene            657..1337
                     /gene="gmk"
                     /locus_tag="AC159P_20580"
     CDS             657..1337
                     /gene="gmk"
                     /locus_tag="AC159P_20580"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - GMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HIR3697897.1"
                     /translation="MSDEAYEIDAEGEELTPGYRGILFVISSPSGGGKGTLIRRVLKD
                     VPGLSYSVSWTTRAPRPGELDGVNYHFVTVEEFERMRDAGGFLEWAVVHGHLYGTALS
                     VVEQDLAEGRDIILEIDVQGAESVRKAMPDVVGVFIMPPSFDVLKMRLLARMTERPEE
                     LALRLSNSRAEVERYAEFDYLVLNDEIERAAAQLAAIVYAERARRERQEWIARRVLAS
                     FRAPAEVE"
     gene            1428..1670
                     /gene="rpoZ"
                     /locus_tag="AC159P_20585"
     CDS             1428..1670
                     /gene="rpoZ"
                     /locus_tag="AC159P_20585"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF013365.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HIR3697898.1"
                     /translation="MAKAAESHNDNDTAQESTETETPVIDSKYRMIIVAAQRSKQLQR
                     GARPRVEMDTQRHKPTRVALEEVQRGKVDFTYTDRD"
     gene            complement(1788..>2424)
                     /locus_tag="AC159P_20590"
     CDS             complement(1788..>2424)
                     /locus_tag="AC159P_20590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011348604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR3697899.1"
                     /translation="AQAIFEALLAEDFSKEQLKRYRQLVRESWITDELRRVRNFHSAF
                     RHGRWLGLANAGLQFITGGRSWGLFDRASLEPGHEALKKLSDYGYRGDDLEQRYGKLR
                     FDNKLTFTKVTDVYHAAVAHDEDQPAHLHVLDTDICSTRCAEEYGNPCQRFCPAAVYE
                     MVEDGRPDGRKRLQINFSNCVHCKTCDIMDPYQIINWVTPEGGGGPDYKGM"
BASE COUNT          434 a          800 c          801 g          389 t
ORIGIN      
        1 gatgtacgag ctgaaccgcc cgctcatcgc cggctacatc caggcgatgc gcgtcatgca
       61 gcaggagttc gggctggcgg gagagccgga catcaacgtg ctggcgcggc tgccgggcgc
      121 gctccaggct tcgcgcgagg gcgtcggccc ggaggtctac gcgggcgtgg agctcgcgct
      181 ggtcggggcg ctcgactagc ttgaggcgat tcgcgagagc gagggcgcgg cgctcgccgc
      241 cgagatgcgg acgcggctcg acgagatcga gcaccacgtg cccatcatcg agtcggtcgc
      301 gggcgggcag ctcgacacct accgcgcgcg cgtctacaag cgcatcacgg aactcctggc
      361 gcgcgacggc cttgagatcg tcgaactcga tcagggacgg ctcgcgcagg aggtcgccta
      421 cctcgccgac cgcagcgatg tcaccgaaga gctggcgcgc ctgaagagcc acatcacgca
      481 gttccgcgag accatcgacg cggggggcga ggccggcaag cggctcgact tcctcttgca
      541 ggagttgaac cgcgaggcca acacggtgct ctcgaagtcc accgacctcg cgatcaaaga
      601 gtcggcgctc gccatcaagg ccgccgtcga gaagctacgc gagcaggtgc agaacgttga
      661 gtgacgaggc ctacgagata gacgccgaag gcgaggagct gacgccgggc taccgcggca
      721 tcctcttcgt catcagctcg ccctcgggcg gcggtaaggg cacgctcatc cgccgcgtgc
      781 tcaaggacgt gcccggtctc agctactcgg tctcgtggac gacgcgcgcg ccccgccccg
      841 gcgagctgga cggcgtcaac taccacttcg tcaccgtcga agagttcgag cgcatgcgcg
      901 acgccggggg cttcctcgaa tgggccgtcg tccacggaca cctctacggc acggcgctca
      961 gcgtcgtcga gcaagacctc gccgaggggc gcgacatcat cctcgaaatc gacgtgcagg
     1021 gcgccgagtc cgtccgcaag gcgatgcccg atgtggtcgg cgtcttcatc atgccgccgt
     1081 cgttcgacgt gctcaagatg cgcctgctcg cgcggatgac cgagcggccc gaagagctgg
     1141 cgctgcggct ctcgaactcg cgcgccgagg tcgagcgcta cgcggagttc gactacctcg
     1201 tgctgaacga cgagatcgag cgcgcggcgg cgcagctcgc ggcaatcgtc tacgccgagc
     1261 gcgcgcggcg cgagcggcag gagtggatcg cccgccgcgt gctggcctcc ttccgcgcgc
     1321 ccgccgaggt tgaataaatc gcgcgaaacg tttataaaaa tcaggttgac ttaaccggca
     1381 tcatccgctg cttgattggt gagtgacacc gaggacgagg attgcacatg gcgaaagctg
     1441 ccgaatcgca caacgacaac gacaccgcac aggaatcgac cgagaccgaa acacccgtga
     1501 ttgactcgaa ataccgcatg attatcgtcg cggcgcagcg cagcaagcag ctccagcgcg
     1561 gcgcgcgccc gcgcgtagag atggacaccc agcgccacaa gcccacgcgc gtggccctcg
     1621 aagaagtgca gcgcggcaag gtagacttca cctacaccga ccgcgactga cccttcaccc
     1681 cgaacagaag gggttaaaag gggaagaggg gaagaggaag acgagagacg tgctgtttct
     1741 cgtcttccct ttcccctttt ccccttttga ccttttcccc cttccgccta catccctttg
     1801 tagtccggcc cgccgccgcc ttcgggcgtc acccagttga tgatctggta gggatccatg
     1861 atgtcgcagg tcttgcagtg gacgcagttg gagaagttga tttgcaggcg cttgcgaccg
     1921 tcgggccgcc cgtcctcgac catctcgtag acggcagccg ggcagaagcg ctggcacggg
     1981 ttgccgtact cttcggcgca gcgcgtcgag cagatgtcgg tgtcgagcac gtgcaggtgc
     2041 gcgggctggt cttcgtcgtg cgcgaccgcc gcgtggtaca cgtccgttac cttcgtgaag
     2101 gtgagcttgt tgtcgaagcg gagcttgccg tagcgctgct ccaagtcgtc gccgcggtaa
     2161 ccgtagtccg agagcttctt caaagcctcg tgccccggct caaggctcgc gcggtcgaac
     2221 aggccccacg agcgaccacc cgtgatgaat tgcaggccgg cgttcgccag ccccagccac
     2281 cgcccgtgcc ggaaggccga gtggaaattg cggacgcgcc gcagctcgtc cgtgatccag
     2341 ctctcgcgga cgagctgcct gtagcgcttg agctgctcct tcgagaagtc ttcggccagc
     2401 agcgcctcga agatggcctg cgcg
//
LOCUS       DBNGOH010001056         5377 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3459972, whole genome shotgun sequence.
ACCESSION   DBNGOH010001056 DBNGOH010000000
VERSION     DBNGOH010001056.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5377)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5377)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5377
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3459972"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            119..2227
                     /locus_tag="AC159P_20595"
     CDS             119..2227
                     /locus_tag="AC159P_20595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697900.1"
                     /translation="MSPSKSRRYSHVPERLRAVLAHEFSQGAQALALFDRFLPLTSYS
                     GEFASDLMDVARGRTGATWEMRRLAALMLENQVLKLAPGDTQEFDLLLVRLGLKAATG
                     KGHEVNGSVLREGYSTTHFCRFVAEFRRRLARLSRVHERLAGARTSPEALRDFIHLSR
                     SDCKISVARYFFKPEEVVARIVGQLQTSRGVPDMVHQEPLYVEREAARALGLLPDYEA
                     EILRRLCAAPLIYWVSKATGSALNSLVEYPLTTVVLVVKPPGSPVEFEIKRAGKRRGP
                     ALSVVYERGGYTVPPPHRLDGGSMLNFLRHEARSGALFSIVYRQAHGTEASMSRMVSR
                     SSIYGVPRNGTVENILEYFTNPEHFGAGFRDMRAAMKDCVYAFNERDPEGAVKIPGPF
                     GLAAMFLHKAVPGQAVLCGTSSFRLDRVAFYLSKDGPEFYFKEGLRTDYTRADARRLA
                     DEVLEEALGVYTPPAVPYRGHRQYVAAAFAAGENRARADRVFLDSMRQLGRYWGTMLA
                     ARGYSWGESFVARNVGLKSYWQDGEWRVRLVFMDHDCLNLSDRSDKDFHSLHAYNGMA
                     TDARYIGGDLIYTTERQTAVHYLGRIYHVSDEVREEGRRLLYDSIRKAYKATHVAMLT
                     NPKLGKFFHKVFLERLRDWDKLVGSYFEMRANGADVEAWKRKVRRTLKEKGYARNVIE
                     GHLKTVESHSDFLRNHSFIY"
     gene            complement(2201..2974)
                     /locus_tag="AC159P_20600"
     CDS             complement(2201..2974)
                     /locus_tag="AC159P_20600"
                     /inference="COORDINATES: protein motif:HMM:NF016377.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR3697901.1"
                     /translation="MRGLLTPRGHRMSNHVVQGLWIGPELSVMERLSAASFMAHGHEY
                     HLYVYDDVRGVPAGVRVRDADEILPASMIFQYSHSKSYAGFANHFRYQLLFDRGGWWA
                     DTDVICVKPFSFDDEHVISTEEHKGAPFANNGVMKAPAGSPLMDYLCGVCRAKDPEQL
                     VWGETGPKLLDEALRQFGLEHYLKPPRVFCPLGYLEWEKALDADAPPALDEETHAVHL
                     WNEMWRRAERDKNARHAHGCLYERLKRKYLGSVDEAVVP"
     gene            complement(2959..3927)
                     /locus_tag="AC159P_20605"
     CDS             complement(2959..3927)
                     /locus_tag="AC159P_20605"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family protein"
                     /protein_id="HIR3697902.1"
                     /translation="MSAIKLLVSELGSWKRYVSREYYHVMTDLMTNHGWRHIETAELF
                     KGVGTVRGRLLGRLGELPRVILFWESYYCLYTHAPDVIRLDCRKCIFADDLKWFRYRS
                     EWLKRVASYSMCDTILASYAYAFDRFYPHLAGRVRVAWVPHAASPDFMLDYNPRPANA
                     ILLSGALNQHYPLREQMMRLREGGRHAITYHPHPGYHCGYDYAADARVGQGYAARIRS
                     HRAGFTDSTRYGYLVAKYFEIPATGALLLADDALGAELKELGLVADRHYVPVSPRDLE
                     EKVRFVLDERNHPELDRVRKRGQELIRERHKTGDRARLIDRICAAS"
     gene            complement(4010..>5377)
                     /locus_tag="AC159P_20610"
     CDS             complement(4010..>5377)
                     /locus_tag="AC159P_20610"
                     /inference="COORDINATES: protein motif:HMM:NF013607.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase/protein-tyrosine-phosphatase
                     family protein"
                     /protein_id="HIR3697903.1"
                     /translation="LSGLGTAIIIGATTLIFTFDGVSIVLALVLMRGGVLVMGPVVDR
                     LFKRRVRWFAWAAFSVTVPAILIALADVNNYSLSATTVAIIVSYLVGYLLRIPCATWL
                     AKSRDEATTYRYFVEEQLVAVVFLVAIPAAFALLGGGEIASELRQGFTGFFTSSTTLP
                     ALVIGALYACLYFFGTLIYLDCRENTYCIPLNRGSSLLAGLFASYVLTLFFGLTPPSG
                     AQVFGAGLLVVALLLLSPLHHRLEHALDKLRPSLAALDLRLRGFAAGLRPFGAPTMMA
                     KVVTANQPGAGDEVYVSDRQPRRRLFLFVCSGNTCRSPMAAAIANAEIAARLGVPLAS
                     LDTLGARTTSAGVSVRVGAPMTDAAQRTLNHLNVPAPPHAARNLTEELALEAEMIFCL
                     TRAHQRAVVELFPSVAAKTHCLDPDGDIEDPIGSGPETYLSCAERIRALVRWRLDEAR
                     ESASF"
BASE COUNT          905 a         1802 c         1807 g          863 t
ORIGIN      
        1 gggacggcgg gcatcgcccg cctctgcgga cgattgacgc ccgcaccgac acggccctca
       61 agggagggcc gcccctacgt cgaaacacac cgcggcgaaa agcggaaggc ccccaaggat
      121 gagtccctcg aagagtcgtc gatacagcca cgtgccggag cgtctccgcg ccgtgctcgc
      181 gcacgagttc agtcagggcg cgcaggcgct cgccctgttc gaccggttcc tcccgctcac
      241 gtcctactcc ggagagttcg cttcggactt gatggacgtg gcgcgcgggc gcacgggcgc
      301 cacttgggag atgcggcggc tcgccgcgct gatgctcgaa aatcaggtgt tgaagctcgc
      361 gcccggcgac acgcaggagt tcgacctcct gctcgtgcgc ctcggcctca aggccgcgac
      421 gggcaagggc cacgaggtca acggctccgt gctgcgcgag ggctactcga cgacgcactt
      481 ctgccgcttc gtcgcggagt tccgccggag gctcgcccgg ctcagccgcg tccacgaacg
      541 gctcgcgggc gcgcggactt cgccggaggc gctgcgcgac ttcatccacc tgtcccggag
      601 cgactgcaag atttcggtcg cgcggtactt cttcaagccg gaggaggtcg tcgcgcgcat
      661 cgtcggccag ttgcagactt cgcggggcgt ccccgacatg gttcatcagg agccgctcta
      721 cgtcgagcgc gaggcggccc gcgcgctcgg gctgctgccc gactacgagg cggagattct
      781 gcgaaggctc tgcgccgcgc ccctcatcta ctgggtctcg aaggccaccg gctccgcgct
      841 caactcgctc gtcgagtacc cgctgacgac cgtcgtgctg gtcgtcaagc cgccgggcag
      901 ccccgtcgag ttcgagatca agcgggccgg caagaggcgc ggccccgcgc tgagcgtggt
      961 ctacgagcgg ggcggctaca ccgtcccgcc gccgcaccgc ctcgacggcg ggagcatgct
     1021 caacttcctg cgccacgagg cgcgctcggg cgcgctcttc tccatcgtct accggcaggc
     1081 gcacggcacc gaagcgtcga tgtcgcggat ggtctcgcgc tcctccatct acggcgtgcc
     1141 gcgcaacggc accgtcgaga acatcctcga atacttcacc aaccccgaac acttcggcgc
     1201 cggcttccgc gacatgcgcg cggccatgaa ggactgcgtc tacgccttca acgagcgcga
     1261 ccccgagggc gccgtcaaga ttcccggccc cttcgggctg gccgcgatgt tcctccacaa
     1321 ggccgtgccc ggccaggccg tgctctgcgg caccagctcg ttccgcctcg acagggtcgc
     1381 cttctacctc tccaaggacg ggccagagtt ctacttcaag gagggcctgc gcacggacta
     1441 cacgcgcgcc gacgcgcggc ggctggccga cgaggtgttg gaagaggcgc tgggcgtcta
     1501 cacgccgccc gccgtgccgt atcgcggcca ccggcagtac gtcgcggcgg ccttcgcggc
     1561 gggcgagaac cgcgcgcgcg ccgaccgcgt tttcctcgac tcgatgcggc agctcgggcg
     1621 ttactggggg acgatgctgg ccgccagggg ctactcgtgg ggcgagtcgt tcgtcgcccg
     1681 caacgtcggc ctcaagagct actggcagga cggcgagtgg cgcgtccggc tcgtcttcat
     1741 ggatcacgac tgcctcaacc tgtccgacag gtccgacaag gatttccact cgctccacgc
     1801 ctacaacggc atggccaccg acgcgcgcta catcggcggc gacctcatct acaccaccga
     1861 gcggcagacc gccgtccact acctcggacg gatctaccac gtctcggacg aggtgcgcga
     1921 agagggcagg cgcctgctct acgattcgat ccgcaaggct tacaaggcca cgcacgtcgc
     1981 catgctgacc aacccgaagc tcggcaagtt cttccacaag gtcttcctcg agcggcttcg
     2041 tgattgggac aagttggtcg ggagctactt cgagatgcgg gccaacggcg ccgacgtcga
     2101 ggcgtggaaa cgcaaggtga ggcggacgct caaggagaag ggctacgccc gcaacgtgat
     2161 cgagggccac ctcaagacgg tggaatcgca ctccgacttt ctacggaacc acagcttcat
     2221 ctactgaccc gaggtatttc cgcttgagcc gctcgtagag acagccgtgc gcgtgccgcg
     2281 cgttcttgtc tctctccgcc ctcctccaca tctcgttcca caggtgcacg gcgtgcgtct
     2341 cttcgtcgag cgcgggcggc gcgtccgcgt ctaacgcctt ctcccactca aggtatccga
     2401 gcgggcagaa gacccgcggc ggcttgaggt aatgctcaag cccgaactgc ctcaacgcct
     2461 cgtccagcag cttcggcccc gtctcgcccc acaccaactg ctcagggtct ttcgcgcggc
     2521 agacgccgca caggtaatcc atcagcggac ttccggcggg cgccttcatg acgccgttgt
     2581 tggcgaacgg cgcgcccttg tgttcttcgg tggagatgac gtgctcgtcg tcgaacgaga
     2641 aaggcttgac gcagataacg tccgtgtccg cccaccagcc tcctctatcg aacaggagct
     2701 ggtagcggaa gtggttggcg aagccggcgt aggatttcga gtgcgagtat tgaaagatca
     2761 tcgaggcggg gagtatctcg tcggcgtcgc gcacgcgcac gcccgcgggg acgcctcgca
     2821 cgtcgtcgta gacgtagagg tgatactcgt gcccgtgcgc catgaacgag gcggcggaga
     2881 gccgctccat cacggacagc tcggggccga tccacaaccc ttggacgacg tggttgctca
     2941 tacggtggcc gcgcggcgtc aggaggccgc gcaaatccgg tcgatcaaac gcgcgcggtc
     3001 gccggtcttg tgccgctcgc ggatcagctc ctgcccgcgc ttccggacgc ggtcgagttc
     3061 gggatgattc cgctcgtcga ggacgaagcg cactttctct tcgaggtcgc gcggcgagac
     3121 gggcacgtaa tgccggtcgg cgacgagtcc cagctccttc agctcggcac cgagcgcgtc
     3181 gtccgccagc aggagcgcgc ccgtggcggg tatctcgaaa tacttggcga cgaggtagcc
     3241 gtacctcgtg ctgtcggtga agcccgcgcg gtggctgcgg attctcgcgg cgtaaccctg
     3301 gccgacgcgc gcgtccgcgg cgtagtcgta gccgcagtgg tagccggggt gagggtggta
     3361 ggtgatcgcg tggcgaccgc cctcgcgtaa gcgcatcatc tgctcgcgga gagggtaatg
     3421 ttgattgagg gcgccgctca gcaggatcgc gttcgcgggg cgcgggttgt agtcgagcat
     3481 gaagtcgggc gaggccgcgt gcggcaccca cgccacccgc acgcgcccgg cgaggtgcgg
     3541 gtagaatctg tcgaaggcgt aagcgtaact ggcgaggatc gtgtcgcaca tcgaatagga
     3601 cgcgaccctc ttcagccact cgctccggta gcgaaaccat ttcaggtcgt cggcgaagat
     3661 gcacttccgg cagtcgaggc ggatcacgtc cggggcgtgc gtgtacagac aatagtagct
     3721 ctcccagaag aggatgaccc gtggcagctc gccgagcctg ccgaggaggc ggcccctgac
     3781 cgtccccacg cccttgaaca gctcggcggt ctcgatgtgc ctccacccgt gattcgtcat
     3841 gaggtcggtc atgacgtggt agtactctcg gctgacgtac ctcttccaac tgcctaattc
     3901 ggagacgagc agctttatcg cggacacgac cctttccgtc ctcggcgggt tgacgatgca
     3961 tcacgcgccg gcggtctttt gacacgaccc cggagccgcg ggcgcggtct cagaaactcg
     4021 cgctctctcg tgcctcgtcg aggcgccacc tgaccagcgc gcggatgcgc tcggcgcagc
     4081 tcaggtaagt ttcaggcccg ctgcctatcg ggtcttcgat gtcgccgtcg gggtcgaggc
     4141 agtgggtctt cgcggcgacg gacgggaaca gctcgacgac cgcgcgttgg tgcgcgcgcg
     4201 tgaggcaaaa aatcatctcg gcttcgagcg ccagctcctc cgtcaggttg cgcgcggcgt
     4261 gcggcggcgc gggcacgttc aagtgattca aagtgcgctg cgctgcgtcc gtcatcggcg
     4321 ccccgactct gaccgagacg cccgcgctcg tggtccgcgc gcccaacgtg tcgagcgacg
     4381 ccaacggcac gccgaggcgc gcggcgatct ccgcgttggc gatggccgcc gccatcggcg
     4441 agcggcaggt gttgccgctg cacacgaaaa ggaagaggcg ccgtcgtggt tgccggtcgc
     4501 tcacgtacac ctcgtcgccg gctccgggtt gattggccgt cacgaccttc gccatcatcg
     4561 tcggcgcgcc gaacggtctc aggccagccg cgaagccgcg gagccgaagg tcgagtgcgg
     4621 cgagcgaagg tctgagtttg tcgagcgcgt gctcgagccg atgatgaagc ggcgacagga
     4681 ggagcagcgc cacgacgagc agccccgcgc cgaagacctg cgccccgctc ggcggcgtca
     4741 ggccgaagaa cagcgtcagc acgtaggacg cgaagaggcc cgcgagcagg cttgagccgc
     4801 ggttcaaagg gatgcagtaa gtgttctcgc ggcagtcgag gtagatgagc gtgccgaaga
     4861 aatagagaca cgcgtacaga gcgccgatga cgagtgcggg cagggtggtg ctgctcgtga
     4921 agaagcccgt gaagccctgc cgcagctccg aagcgatctc gcctccgccc aacagggcga
     4981 aggcggcggg tatcgccacc agaaagacga ccgcgaccaa ctgctcttcg acgaagtagc
     5041 ggtaagtggt cgcctcgtcg cgcgacttcg caagccatgt ggcgcaggga attcgcagga
     5101 ggtagcccac gaggtaagag acgatgatgg ccaccgtcgt ggcggacagg ctgtagttgt
     5161 tcacgtcggc cagcgcgatc agtatcgcgg gcaccgtgac cgagaaggcc gcccacgcga
     5221 accagcggac gcggcgcttg aagaggcggt caacgaccgg ccccatgacg agcacgcccc
     5281 cgcgcatcag caccagcgcg aggacgatgg agaccccgtc gaaggtgaag atcagcgtcg
     5341 tcgccccgat gatgatggcc gtgccgaggc ccgagag
//
LOCUS       DBNGOH010001057         7678 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2544284, whole genome shotgun sequence.
ACCESSION   DBNGOH010001057 DBNGOH010000000
VERSION     DBNGOH010001057.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7678)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7678)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7678
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2544284"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..271)
                     /locus_tag="AC159P_20615"
     CDS             complement(<1..271)
                     /locus_tag="AC159P_20615"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018791606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3697904.1"
                     /translation="MLIREYTNTDWPSVWPIFQEVVNAGDTYAYDPLWSSEQARGVWV
                     EAPPGRTVVACDGSSVLGTAKMGPNRPGPGSHVATASFMVAAAARG"
     gene            398..1306
                     /locus_tag="AC159P_20620"
     CDS             398..1306
                     /locus_tag="AC159P_20620"
                     /inference="COORDINATES: protein motif:HMM:NF012515.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HIR3697905.1"
                     /translation="MDTSFTVVGLGELIWDMLPAGRQLGGAPSNFAYVSRLLGNRAVV
                     ASRVGDDALGREAVARLARVGLETSFIQRDAAHPTGTVGVKIDTHGEAHFTVNPNSAW
                     DYLEWTPAWQQLAARADAVCFGTMGQRELRARETIARFLGHTRAGAVRLFDVNLRHTF
                     FTPDMLARSLEAATLVKLNNHECAEVARMLRAPARDELATARHLIRAFGISLVAVTRG
                     DAGSLLVTADETSEHHGFHQPRVADTIGAGDAFAAALAHARLKGASLDKISAAANRLG
                     AWLVTQCGATPPPDRQLLDTVARMLE"
     gene            1364..3541
                     /locus_tag="AC159P_20625"
     CDS             1364..3541
                     /locus_tag="AC159P_20625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020706437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconolaconase"
                     /protein_id="HIR3697906.1"
                     /translation="MRGRIILLILLVIIPGTAALILLRSAREARRAPTPLGWHAAVST
                     RAGDGAPGTRDDAQSSRASFADPFGLAVGADGTLYVSDGGESNRIRRLAPDGALTTLA
                     GGAEGFADGAGTAASFNTPSGLAIDAEGNLYIADTGNNRVRRVTPEGQVSTVAGEGTA
                     GHADGPAASARFDAPVGVAVDRRGNVYVADTYNDRVRKITPAGQVTTVAGAGRPGYAD
                     GPANDALFDTPCALVVNEDGTLIVADTGNNRLRKITTDGQVTTLPINFPADASTELSD
                     PAGLALTHDGFLYVTEMDRSRVTQVAPDGAARVIAGGGPGYADGDGLRGARFNQPAGL
                     ALAPGGALYVADGANYLVRKLSPLNSNEDDSNADDRLAADLVAVVNRHAVGEPLPRLT
                     PATLGEQALLWPLDPQRSPHEVAATLGEVRGSYDTDDSRHHLHSGLDVFGAYGDTVRA
                     VRSEKVAAPLANWGFGSLNEGMRVGIISYIHMHVGRDKDANPFDDPRFVAVRGDDGKP
                     ARVRVRRGTRFRTGDAVGTVNRMYHVHMNVGPPGAQVNPLAFAPVGFTDRVAPRIERE
                     GIQLFDESGARLEEKRDNRLVVRGRVRIVVEAHDRADMNPERRRLGLYRLGYQILTPD
                     LAPAPGFEHPRLNIEFDRLPPDAEATKIAYAAESGITVYGSQTTRFLYEVTNTVRDGR
                     AAHGLFDASALPPGDYVLRVIAADYNGNEATEGRDVLITVEGG"
     gene            complement(3575..4360)
                     /gene="truA"
                     /locus_tag="AC159P_20630"
     CDS             complement(3575..4360)
                     /gene="truA"
                     /locus_tag="AC159P_20630"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HIR3697907.1"
                     /translation="MRNWKLEIEYDGTRYSGWQVQPHARTVQGELLKAAETFLKTAVE
                     LGGSGRTDAGVHALRQVAHLKVKSDAAKQTARQLEVGLNGLLPHDINVLKAEAAPPRF
                     HARHDAVARYYLYRISTRRTAFGKSYVWWVRDRLNVAAMTAACAALAGRHDFHSFCES
                     PEKQGSTVVHVDAARVFAAGDLILFRIGASHFLWKMVRRLVGALVEVGRGRLTPDAFR
                     ALLDTRSNEPAAWTAPPSGLFLERVLYRGDTPPTDDRHALRFD"
     gene            complement(4588..6477)
                     /locus_tag="AC159P_20635"
     CDS             complement(4588..6477)
                     /locus_tag="AC159P_20635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010932972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HIR3697908.1"
                     /translation="MHILSIEGVGKNYGLKPLFENVSLGLDSADRVGVVGVNGSGKTT
                     FLRLVAGAERPDAGRVVFAEGVSIGYLPQTPAFAPQVSVLEAVFAASDAKTKLLLESE
                     RACQALSASGSEEQRLLDRVSELAHRLEASGAWELETDARMILGRLGITDTAARMGTL
                     SGGQRKRVALAHALIERPDLLILDEPTNHLDAETISWLEGHLGGFRGALLLVTHDRYF
                     LDRVTSRIIEIDRGAVQAFDGGYAYYLEKKEEQEAAREAAGHKREMLMRRELAWLRRG
                     AKARTRKSKARVERAEELIARPREAERAELDISVTSSRLGKKVLELRDISKSYDGRAL
                     IEDFDYIFKPGDRIGVIGPNGAGKTTLLEIIAGRVAPDRGKVETGQTVRLGYYDQENR
                     ALDEEARVIDYVREVAERIETADGAFITAGQMLEKFLFTGAMQYAPIAKLSGGERRRL
                     YLLRVLMGAPNVLLLDEPTNDFDIRTLVTLEDYLDGFAGCLVVVSHDRYFLDRTAEHI
                     FRFEGGGRVRQYPGNYTAFLEARREEERDAAPPPTAKAERPARREPDAPAARRLSFKE
                     RRELEETEARIEAAERRQAAIETELAANASDAHAVHRLYEEREQLARRLARDLDRWAE
                     LAEHA"
     gene            complement(6571..7611)
                     /locus_tag="AC159P_20640"
     CDS             complement(6571..7611)
                     /locus_tag="AC159P_20640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HIR3697909.1"
                     /translation="MFFELSPQSSALSPSVSKLWHMNADFETELAAPPGAYRRTPPFA
                     LLNRRLSPRLLRLARPGDALLIDEPWPDRLRAEAQRRGVELVSTETSGRQGASAERQD
                     ARLFTPWGWTPSAVAAGERAGAVVPSVPLDVVARVNSKLWSHALEVESGIALPGAALA
                     STIEELREAIARACPAPDDKWVVKSPYGFAARDRVLGRGPVLEAAQGRWCERRMARGE
                     TLIFQPWLAVVREYGVVAEIASDGSFEVHGISDLQTNGAGTGTGYILGRPPDAGRARE
                     LTRVASLVCQRLFQEGYYGPVGIDALEHAGGLHPLLEINARYTMGFVALAVEQQLKPA
                     APTFWRTKGNAE"
BASE COUNT         1209 a         2780 c         2515 g         1174 t
ORIGIN      
        1 ggccccgcgc ggcggcggcg accatgaaac tcgcggtggc gacgtgcgag ccggggccgg
       61 gacgattcgg ccccatcttc gccgttccga gcacagagga gccgtcgcag gcgacgaccg
      121 tgcgaccggg aggagcctcg acccacacgc cccgagcctg ctcggacgac caaaggggat
      181 cgtaagcgta agtgtcgccc gcgttgacga cctcttggaa gatgggccag acggacggcc
      241 agtcggtgtt cgtgtattca cgaatcagca tgacgacatc ttaatcgaag ccccgagccg
      301 gagccagagt aaagccataa acccctcttg cgacgcgctg tctcttcgtg tctgcggcgg
      361 cgaaatcctc tgtggtatac attcgtgtgg aacagtcatg gacacttcat tcaccgtcgt
      421 cggcctcggc gagctgatct gggacatgct gcccgcgggc agacagttgg gcggggcgcc
      481 gtcgaacttc gcgtacgtct cgcgcctgct cggcaaccgc gccgtcgtcg ccagccgcgt
      541 cggcgacgac gcgctcgggc gcgaggccgt cgcccgcctc gcccgcgtcg ggcttgagac
      601 ctcgttcatc cagcgcgacg ccgcgcaccc gaccggcacc gtcggcgtga agatcgacac
      661 gcacggcgaa gcgcacttca ccgtcaaccc gaactccgcg tgggattatc tggaatggac
      721 tcccgcgtgg cagcagctcg cggcacgcgc cgacgcggtc tgcttcggca cgatgggcca
      781 gcgtgagcta cgtgcgcgcg agaccatcgc gcgcttcctc gggcacacgc gcgccggcgc
      841 cgtccgcctc ttcgacgtga acctgcgcca taccttcttc acgcccgaca tgctcgcgcg
      901 ctcgctcgaa gccgccacgc tcgtcaagct caacaatcac gagtgcgccg aagtcgcccg
      961 catgctccgc gccccggcgc gcgacgaact cgccaccgcg cgccacctga ttcgcgcctt
     1021 cggcatctcg ctcgtcgccg tcacgcgcgg cgacgcgggg agcctgctcg tcaccgccga
     1081 cgaaacgtcc gagcaccacg gctttcatca accacgcgtc gccgacacca tcggcgcggg
     1141 cgacgccttc gccgccgccc tcgcgcacgc ccgcctcaaa ggcgcctcgc tcgacaaaat
     1201 ctccgccgcc gccaaccgcc tcggcgcctg gctcgtcaca caatgcggcg ccacgccccc
     1261 tcccgaccgc caactactcg acacggtggc gcggatgtta gagtgagcag tgcttcccga
     1321 agagccgaaa aactgacacg agaccgcttc cttgatcgac cacatgcgcg gacgcatcat
     1381 ccttctcatc ctcctcgtca tcatccccgg cacggcagct ctcatccttc ttcgctccgc
     1441 gcgcgaagcg cgtcgcgcgc cgacgcctct cggttggcac gccgccgtca gcacgcgcgc
     1501 gggcgacggc gcgcccggca cccgcgacga cgcgcaaagc tcgcgcgcaa gcttcgcaga
     1561 ccccttcggc ctcgcggtcg gcgcggacgg cacgctctac gtctccgacg gcggcgagag
     1621 caatcgcatc cgcaggctcg cgcccgacgg cgcgttgacg acgctcgccg gcggggccga
     1681 gggcttcgcc gacggcgcgg gcacggcggc ctctttcaac acgccgtcgg ggctggcgat
     1741 cgacgccgaa ggcaacctct acatcgccga caccgggaac aaccgcgtcc gcagggtcac
     1801 gcccgaaggg caggtcagca cggtcgcggg cgaaggcacg gcgggccacg ccgacggtcc
     1861 cgccgcctcg gcgcgcttcg acgcccccgt cggcgtggcc gtagaccggc gcggcaacgt
     1921 ctacgtcgcg gacacctaca acgaccgcgt ccgcaagatc acgcccgcag ggcaggtcac
     1981 gaccgtcgcc ggagcgggcc gacccggcta cgccgacggc cccgcgaacg acgcgctctt
     2041 cgacaccccc tgcgcgctcg tcgtcaacga agacggcacg ctcatcgtcg ccgacacggg
     2101 caacaaccgc ctgcgcaaga tcacgacgga cggtcaggtc acgacgcttc ccatcaactt
     2161 tcccgccgac gcttcgactg aactatccga ccccgccggt ctggcgttga cgcacgacgg
     2221 cttcctctac gtcaccgaga tggacaggtc gcgcgtcacg caggtcgcgc ccgacggcgc
     2281 cgcgcgagtc atcgcgggcg gcgggccggg ctacgccgac ggcgacggcc tccggggcgc
     2341 gcgcttcaac cagcccgctg gactagcgct cgcgcccggc ggcgcgctct acgtcgccga
     2401 cggcgccaac tacctcgtgc gaaaactgtc gcccctgaac tcgaacgaag acgattcgaa
     2461 tgcagacgac cgcctcgccg ccgacctcgt cgccgtcgtc aaccgacacg ccgtgggcga
     2521 accgttgccg cgcctcacgc ccgcgacgct cggcgagcaa gcgctgttgt ggccgctcga
     2581 cccgcagcgg agtccgcacg aggtggcggc gacgttgggc gaggtgcgcg gcagctacga
     2641 cacggacgac agccgccacc acctgcacag cggactcgac gtgttcggcg cctacggcga
     2701 cacggtgcgc gccgtgcgtt cggagaaggt cgccgccccg ctcgccaact ggggcttcgg
     2761 cagcctgaac gaagggatgc gcgtcggcat catctcctac atccacatgc acgtcgggcg
     2821 cgacaaggac gccaacccgt tcgacgaccc gcggttcgtc gcggtgcgcg gcgacgacgg
     2881 caagcccgcg cgcgtgcgcg tccggcgcgg cacgcgcttc cgcacgggtg acgccgtcgg
     2941 caccgtcaac cgcatgtacc acgtccacat gaacgtcggc ccgcccggcg cgcaggtcaa
     3001 cccgctcgcc ttcgcgcccg tcggcttcac agaccgcgtc gccccgcgca tcgaacgtga
     3061 aggcatccag ctcttcgacg agtcgggcgc gcggctcgaa gagaagcgcg acaaccgtct
     3121 cgtcgtgcgc ggtcgcgtac gaatcgtggt cgaagcgcac gaccgcgccg acatgaaccc
     3181 cgagcgccgc cgcctcggcc tctaccgcct cggctatcaa atcctcacgc ccgacctcgc
     3241 gcccgcgccc ggcttcgaac acccgcgcct caacatcgag ttcgaccgcc tcccgcccga
     3301 cgcggaagcg accaagatcg cctacgccgc cgagagcggc atcaccgtct acggcagcca
     3361 gacgacgcgc ttcctctacg aagtgaccaa caccgtccgc gacggtcgcg ccgcgcacgg
     3421 cctcttcgac gcgagcgcgc tgccccccgg cgactacgtg ctccgcgtca tcgccgccga
     3481 ctacaacggc aacgaggcca cggaggggcg cgacgtgttg ataacagtcg aaggcggttg
     3541 agtcacgaga ggtaaagtga ttcgcggtcg cgctttagtc gaagcgtaac gcgtggcggt
     3601 cgtccgtcgg cggcgtgtca ccgcggtaga ggacgcgttc gaggaacagc ccggagggcg
     3661 gcgccgtcca cgccgccggt tcattgctgc gtgtatcgag cagcgcgcgg aaagcgtccg
     3721 gcgtcagcct gcccctgccg acttcgacga gcgcgccgac gaggcgccgc accatcttcc
     3781 acagaaagtg gctcgcgccg atgcggaaga ggatgaggtc gcccgcggcg aagacgcgcg
     3841 cggcatcgac gtggacgacc gtcgagcctt gcttctcggg actctcgcag aaggagtgga
     3901 agtcgtgtct gccggcgagc gccgcgcacg ccgccgtcat ggccgcgacg ttcaagcgat
     3961 cccgcaccca ccagacgtaa gacttgccga acgcggtgcg ccgcgtcgag attcgataga
     4021 ggtagtagcg cgcgacggcg tcgtggcgcg cgtggaagcg cggcggcgcg gcttccgcct
     4081 tcagcacgtt gatgtcgtga ggcagcaggc cgttgaggcc cacctccaac tgccgcgccg
     4141 tctgcttcgc ggcgtccgac ttcaccttca ggtgcgccac ctgccgcagc gcgtggacgc
     4201 ccgcgtccgt gcggcccgag ccgcccagtt cgaccgccgt cttcaggaaa gtctcggcgg
     4261 ccttcaatag ctcgccctgc accgtccgcg cgtgcggctg cacctgccaa ccgctgtagc
     4321 gcgtgccgtc gtactcgatc tcaagcttcc agttccgcat cagtcgaacc ttgataacct
     4381 caagcagcca ggggaatcgc gttagagaga gtgcggcatg cgcgtgaaga ctgtaggggc
     4441 agggcttgtc cctgcccgcc gtcacattcc actcaagatt gaatcaatcg gacgttgacg
     4501 ggccgcggac ttgccctgcc cctacagttg tgccgcgcgt ctcacgtcca ttcgaagact
     4561 gctctactga cagacaggtg tggcagttca agcgtgctct gcgagttcgg cccagcggtc
     4621 gaggtcgcgc gcgagtcgcc gggccaactg ctcgcgctcc tcgtagagac ggtggacggc
     4681 gtgtgcgtcg ctcgcgttcg cggccagctc ggtctcgatt gcggcctgcc gccgttcggc
     4741 ggcctcgatg cgcgcctccg tctcttcaag ctccctgcgc tctttgaacg acagcctgcg
     4801 ggcggcgggc gcgtccggtt ctcgtcgggc ggggcgctcc gccttcgcgg tcggcggcgg
     4861 cgcggcatcc ctctcttcct cgcggcgcgc ttcgaggaag gcggtgtagt tgccgggata
     4921 ctggcgcacc cgtccgccgc cttcgaagcg gaagatgtgt tcagccgtgc ggtcgaggaa
     4981 gtagcggtcg tggctgacga cgacgaggca gccggcaaag ccgtcgaggt agtcttcgag
     5041 cgtgaccagc gtgcggatgt cgaagtcgtt cgtcggctcg tccagcagga gcacgttcgg
     5101 cgcgcccatc agcacgcgca gaaggtagag ccgccgccgc tcgccgcccg acagcttcgc
     5161 gatcggcgcg tactgcatcg cgcccgtgaa gagaaacttt tcgagcatct gcccggcggt
     5221 gatgaacgcg ccgtcggcgg tctcgatgcg ctcggcgacc tcgcggacgt agtcgatgac
     5281 gcgcgcctct tcgtccaaag cgcggttctc ctgatcgtaa taaccgagcc gcacggtctg
     5341 gcccgtctcg accttccctc ggtcgggcgc gacgcgaccg gcgatgattt cgagcagcgt
     5401 ggtcttgccc gcgccgttcg ggccgatgac gccgatgcgg tcgcccggct tgaagatgta
     5461 gtcgaagtct tcgatcaacg cgcgcccgtc gtaggacttc gagatgtcgc gcagctcaag
     5521 caccttcttg ccgaggcggc tcgaagtgac cgagatgtcc agctcggccc gctccgcctc
     5581 gcggggccgc gcgatcaact cctccgcgcg ctcgacgcgc gccttggatt tacgcgtgcg
     5641 cgccttcgcc ccgcggcgca gccacgccag ctcgcgccgc atcagcatct cgcgcttgtg
     5701 ccccgcggct tcgcgcgccg cctcctgctc ctccttcttt tcgaggtagt aagcgtagcc
     5761 gccgtcgaac gcctgcaccg cacccctatc gatctcgatg atgcggctcg tcacgcggtc
     5821 gaggaagtag cggtcgtgcg tgacgagcag gagcgcgccg cggaagccgc cgaggtggcc
     5881 ttcgagccac gagatggtct cggcgtcgag gtggttggtc ggctcgtcga ggatgagcag
     5941 gtctggccgc tcgatgagcg cgtgcgcgag cgcgacgcgc ttgcgctggc cgcccgagag
     6001 cgtgcccatc cgcgcggcgg tgtccgtgat gccgagcctg ccgagaatca tccgcgcgtc
     6061 cgtctcaagc tcccacgcgc ccgacgcttc gaggcggtgc gccaactccg acacgcgatc
     6121 aagcagacgt tgctcttcac tgccgctcgc cgacaacgcc tgacacgcgc gctcggattc
     6181 gagcagcagc ttcgtcttcg cgtcgctcgc cgcgaacacg gcttcgagca ccgacacctg
     6241 cggcgcgaac gcgggcgtct gcggcaggta gccgatggac acgccctcgg cgaagacgac
     6301 gcgccccgcg tccggcctct ccgcgcccgc gacgagacgc aggaaagtcg tcttgcccga
     6361 accgttcacg ccgaccacgc ctacgcggtc ggcggaatcg aggccgaggc tcacgttctc
     6421 gaacaggggc ttcagcccgt aattcttgcc gaccccttcg atggataaaa tgtgcatgcc
     6481 ctcagaagaa atgcagaatg atgaatgatg aatgagaaga acgctgcctc tcatcactca
     6541 acttgtcttt gattcatcat tcatcattca tcattctgca ttgcctttcg tgcgccagaa
     6601 cgtcggcgcg gcgggcttga gctgctgctc gacggccagc gcgacgaagc ccattgtata
     6661 gcgcgcgttg atttccagca gcgggtggag gccgcccgcg tgttcgagcg cgtcgatgcc
     6721 gaccggcccg tagtagcctt cctgaaagag acgttgacag acgagtgaag cgacgcgcgt
     6781 caactcgcgc gcgcgtcccg cgtcgggcgg gcgaccgagg atgtagccgg tgcccgtgcc
     6841 tgccccgttg gtctgcaagt cgctgatgcc gtgcacctcg aaagagccgt ccgacgcgat
     6901 ctccgccacg acgccgtact cgcgcacgac cgcgagccac ggctggaaga tcagcgtctc
     6961 gccgcgcgcc atccggcgtt cgcaccaacg gccttgcgct gcttccagca caggtccgcg
     7021 gcccagcacg cggtcgcgcg cggcgaagcc gtaagggctt ttcaccaccc acttgtcatc
     7081 cggcgcggga cacgcgcgcg cgatggcttc gcgcagctct tcgatggtcg atgcgagggc
     7141 cgcccccggc aacgcgatgc ccgactcgac ttcgagcgcg tggctccaca gcttcgagtt
     7201 gacgcgggcc accacgtcga gcgggacgga cgggacgacg gcgcccgccc tctcgcccgc
     7261 ggcgacggcg ctcggcgtcc agccccacgg cgtgaagagg cgcgcgtcct gtcgttcggc
     7321 gcttgcgccc tgtcgcccgg aagtttcggt cgagacgagt tcgacgccgc ggcgttgtgc
     7381 ttccgcgcgc aggcggtcgg gccacggctc gtcgatgagc agcgcgtcgc cggggcgcgc
     7441 gagccggagc aggcgcgggg agagtctgcg gttgaggagt gcgaacgggg gcgtgcggcg
     7501 gtaggcgccg ggcggggcgg ccagctccgt ctcgaagtcg gcgttcatgt gccagagctt
     7561 cgagacggaa gggctgaggg ctgaggactg agggctgagt tcgaagaaca tgctttcact
     7621 cagccctcag tgcgagccag tcgcgcgcga tgtcgatggt ggcgcggcgc gcggcggg
//
LOCUS       DBNGOH010001058         1705 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_160812, whole genome shotgun sequence.
ACCESSION   DBNGOH010001058 DBNGOH010000000
VERSION     DBNGOH010001058.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1705)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1705)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1705
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_160812"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..717
                     /locus_tag="AC159P_20645"
     CDS             <1..717
                     /locus_tag="AC159P_20645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HIR3697910.1"
                     /translation="QELFSLYHNERDLSYVDSSGEIGSATRLLSGWGLKFFDYDNDGD
                     PDLFLANGHPDDMVDMRAVNVKYREPLLMFENTGRSFKNVSAQSGPVFGKDFPARGMT
                     VGDYDNDGDLDVLVGNNGEPPLLLRNEGGNRNNWLGLQLHTTRSGPGATGAVITWQAG
                     GTKRSRLKTGGGSFLASHDPREVLGLGRAAKVDSLEVRWPSGKVDRLSDLTANTYVTI
                     VEGEGALKGASKPGKPTLRR"
     gene            725..>1705
                     /locus_tag="AC159P_20650"
     CDS             725..>1705
                     /locus_tag="AC159P_20650"
                     /inference="COORDINATES: protein motif:HMM:NF024299.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3697911.1"
                     /translation="MRDGGAETVNGLAVLRPRGFSRLPKATVLTLGACCLALLLYLPA
                     VGQRRQAGGGRKQPAISQQDFDRLTAQADEARGGGRFDEAIGLYRRALGARPRWAEGW
                     WLLATLLYERDEYAEAARSFAQAAELQPKAGAAWAMLGLCEYRLGRYDAALGHLHRAR
                     RIGLANNPELIRVMIYHEGLLLNLRGDFETAHQRFGALAYEGLSSEDLLIALGLSALR
                     IPVTPAQVGPDHRDRALIRRVGWAEFQSAQKNRSDAQGEYERLIADFPRTPNVQYAFG
                     RFLLNTRRDEEGALAAFQREIENAPDSAVARIQIAYLKLKSKEAAAGIPYV"
BASE COUNT          287 a          581 c          600 g          237 t
ORIGIN      
        1 caggagctct tcagcctgta ccacaacgag cgggacctca gctacgtcga ctcgtcgggc
       61 gagatcgggt cggcgacgcg cctgctgagc ggctgggggc tgaagttctt cgactacgac
      121 aacgacggcg acccggacct cttcctcgcc aacggccacc cggacgacat ggttgacatg
      181 cgcgccgtca acgtcaagta cagggagccg ctcctcatgt tcgagaacac gggccgctcc
      241 ttcaagaacg tgagcgcgca gtccggcccg gtcttcggga aagacttccc ggcgcgcggc
      301 atgacggtcg gcgactacga caacgacggc gacctcgacg tgctcgtcgg caacaacggc
      361 gagccgcccc tgctcttgag gaacgaaggc ggcaaccgga acaactggct cgggcttcag
      421 ttgcacacga cccggagcgg ccccggcgcg acgggcgcgg tcatcacctg gcaggccggc
      481 ggcacgaagc gcagccgctt gaagacgggc ggcggcagct tcctcgcgtc acacgacccg
      541 cgcgaggttc tgggtctggg gcgcgcggcg aaagttgaca gcctcgaggt acgctggccg
      601 agcggcaagg tcgatcggct ctccgacctg acggccaaca cctacgtcac aatcgtcgag
      661 ggggagggcg cgctcaaggg cgcgtcgaag cccggcaagc cgacgcttcg gaggtgaagt
      721 aacgatgcgg gacggcggcg ccgaaacggt gaacgggctg gcggttcttc gcccgcgggg
      781 gttttcgcgc ctcccgaagg ccaccgtgct caccctcggg gcctgctgtc tcgccctcct
      841 cctgtatctt ccggcggtgg ggcagcggcg gcaggcgggc ggcggacgga agcagccggc
      901 gatttcgcag caggatttcg accgcctcac ggcgcaggcg gacgaggccc gcggcggcgg
      961 tcgcttcgat gaggcgatag gactctaccg gagggcgctc ggcgcgcgcc cccgctgggc
     1021 cgaagggtgg tggctgctcg ccacgctgct ctacgagagg gacgaatacg ccgaggcggc
     1081 gcggagcttc gcacaggccg ccgaacttca accgaaggcg ggggcggcgt gggcgatgct
     1141 cggtctgtgc gaataccgcc tgggccgcta cgacgccgcg ctcggccacc tacaccgggc
     1201 gcgccggata gggctggcga acaacccgga gttgatccgc gtgatgattt atcacgaggg
     1261 gctgctcttg aatctgaggg gtgacttcga gaccgcgcac cagaggttcg gcgcgctcgc
     1321 ctacgagggc ctgagcagcg aagacctgct catcgccctc ggcctctcgg cgctgcgcat
     1381 cccggtcact ccggcgcagg tcggccccga ccaccgcgac cgcgctctga tccgccgcgt
     1441 cgggtgggcc gagttccagt cggcgcagaa gaaccgttcg gacgcgcagg gcgagtacga
     1501 gcgcctgatc gcggacttcc ccaggacccc caacgtccag tacgccttcg ggcgcttcct
     1561 cctcaacacg cggcgcgacg aggagggcgc gctcgcggcc ttccagcgcg agatcgagaa
     1621 cgcgcccgac agcgccgtcg cgcggataca gatcgcctac ctgaagctca agagcaagga
     1681 ggccgccgcc gggatacctt acgtc
//
LOCUS       DBNGOH010001059         3463 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_344773, whole genome shotgun sequence.
ACCESSION   DBNGOH010001059 DBNGOH010000000
VERSION     DBNGOH010001059.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3463)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3463)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3463
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_344773"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="AC159P_20655"
     CDS             complement(<1..188)
                     /locus_tag="AC159P_20655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005945906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697912.1"
                     /translation="MAHIAELSGAGREAVVNGGRLKAAILGATGMVGQQFVRVLKSHP
                     WFEVVALAASSSSAGKSYG"
     gene            complement(291..1082)
                     /locus_tag="AC159P_20660"
     CDS             complement(291..1082)
                     /locus_tag="AC159P_20660"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3697913.1"
                     /translation="MAENYRINTSLPRDLLRGRVGLVTGGSRGVGRSVALRLAEAGAN
                     VVVSYARREAAAQQVARSCAEFGVEAVAVRADVCDLKQAQALVTAAVERFGRLDLLVA
                     NAGIWEGAPVEEMSEELWSRVIDANLKGTWAACRAAVPVMKKQKRGNIVIISSTAGQR
                     GEAGYSNYAASKGGQISFTKSLAAELAPDIRVNAVAPGWIDTEMNESVFGDEEYKRKV
                     IDAIPLRRIATADDVALSVLFLASDWSQHVTGEILNVNGGAVLCG"
     gene            complement(1107..2276)
                     /gene="metX"
                     /locus_tag="AC159P_20665"
     CDS             complement(1107..2276)
                     /gene="metX"
                     /locus_tag="AC159P_20665"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase MetX"
                     /protein_id="HIR3697914.1"
                     /translation="MSTTIEPTIEADCTFARDAAFRVSGGELRPLTLRYAMYGELNER
                     RDNAILVCHALSGSARVADWWPELFGAGGVFDSTRHCVIGVNVIGSCYGSTGPGSINP
                     RTGEPYGADFPLVSIADMVRAQARLLDRLGVERLRAVVGGSIGGMQALRWAVDFPERV
                     ERCIAVGAAPLGAMGLALNHLQRQAITNDPAWRGGRYDADAPPVAGLALARGLAMCSY
                     KSAELFDGRFARRPDRAGEDPHRSLGERFDIAGYLDYQGRAFTGRFDANSYLVLTKAM
                     DTFDLARGHESEAAALRRVAARVLLVGITSDWLFPADSVRALAARMASAGADCRYEEL
                     RSAHGHDGFLADADLLAPLLGAALEDDGRATREGHTAGAGRTRTRARSAAALGAA"
     gene            complement(2428..>3463)
                     /locus_tag="AC159P_20670"
     CDS             complement(2428..>3463)
                     /locus_tag="AC159P_20670"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HIR3697915.1"
                     /translation="SDSNKDGGMLTSTWEIYKAHRALHRVASECDVRLRLFHGRGGTV
                     GRGGGPTHRAIVAQPAGAFGGQFKITEQGEVLNWKYSEPVLAERNLELMVAASLEALS
                     RAAGTPARDELREWEAALEEMSRDAFDFYRANVAENPDILPYFEEATPVLELEHARIG
                     SRPPRRGERRGLEDLRAIPWVFGWMQSRHVLPAWFGVGHALEKFAGTDAARERLLQEM
                     FEGFPLFEDLISNVEIGMAKADFSIAALYAGLVTDEDLRRRVFDLVTEEFRRTRRMLL
                     RVSRQSRLLENNQVLARSIRLRNPYVDSLSLIQVELLRRKRAGEERDELNYALAATIN
                     GVSAGLRNTG"
BASE COUNT          566 a         1230 c         1127 g          540 t
ORIGIN      
        1 ccgtaagact tccccgccga actgctcgaa gccgccagcg cgaccacctc gaaccacgga
       61 tgacttttca gcacgcgcac gaactgctgc cccaccatcc cggtcgcgcc gaggatggcc
      121 gccttcaacc tgccgccgtt aaccacggcc tccctgcccg cgccgctcag ttccgcaatg
      181 tgcgccatct gtaaacctcc aaagaccgtg aaccgtaaac cgtaaaccgc gaaaaaataa
      241 agctgttctg actcttctcg gtttacgatt cacggttcac ggcttttcat ctatccgcag
      301 agcaccgcgc ccccgttgac gttgagtatc tcgccggtga cgtgctgcga ccagtcggag
      361 gcgaggaaga ggacggacag agccacgtcg tccgccgtgg cgatgcgccg gagcgggatg
      421 gcgtcgatca ctttgcgttt gtactcctcg tcgccgaaga ccgactcgtt catctccgtg
      481 tcgatccagc cgggcgcgac cgcgttgacg cggatgtcgg gcgcgagttc ggcggccagc
      541 gacttggtga agctgatctg cccgcccttc gaggcggcgt agttcgagta gcccgcctcg
      601 ccgcgctgcc ccgcggtcga gctgatgatg acgatattac cgcgcttctg ttttttcatc
      661 acgggcacgg cggcgcggca cgcggcccag gtgcctttga ggttggcgtc gatgacgcgg
      721 ctccacagct cctccgacat ctcttcgacg ggcgcgccct cccagatgcc cgcgttcgcc
      781 acgagcaggt cgagccgacc gaagcgctcg accgccgccg tgaccagcgc ctgcgcctgt
      841 ttgaggtcgc acacgtccgc gcgcaccgcg acggcctcga cgccgaactc cgcgcacgaa
      901 cgcgcgacct gctgcgccgc cgcctcgcgc cgcgcgtaac tgacgacgac gttcgcgccc
      961 gcctccgcca gccgcagcgc gaccgagcgc cccacgccgc gcgagccgcc cgtgaccagc
     1021 cccacgcgac cccgcaacaa gtcccgtggg agcgaggtgt tgattcgata attctcagcc
     1081 atgatgtctc caacctccga aagttctcag gcggcgccga gcgcggcagc cgagcgggcg
     1141 cgcgtcctcg tgcgccccgc gcccgccgtg tgtccctcgc gcgtcgcgcg cccgtcgtct
     1201 tcgagcgccg cgccgagcag cggcgcgagc aagtcggcgt ccgccagaaa gccgtcgtgc
     1261 ccgtgcgccg aacgaagctc ctcgtaacga cagtcggccc cggctgaagc catcctcgcg
     1321 gccagcgcgc ggacgctgtc agccgggaag agccagtccg aagtgatgcc gaccagcagg
     1381 acgcgtgccg cgacgcgccg cagcgccgcc gcctcggact cgtggccgcg cgcgaggtcg
     1441 aacgtgtcca tcgccttcgt caggacgagg tacgagttgg cgtcgaagcg ccctgtgaac
     1501 gcgcggccct gatagtcgag gtagcccgcg atgtcgaagc gctcgccgag cgagcggtgc
     1561 gggtcttcgc ccgcgcggtc gggccggcgc gcgaaccgcc cgtcgaacag ctccgcggac
     1621 ttgtaagagc acatcgcaag gccccgcgcg agcgcgaggc cggcgaccgg cggcgcgtcc
     1681 gcgtcgtagc gaccgccgcg ccacgccggg tcgttcgtga tggcctgccg ttgcaggtgg
     1741 ttgagcgcga ggcccatcgc cccgagcggc gcggccccga cggcgatgca cctctcgacg
     1801 cgctcgggga aatcgaccgc ccacctcagc gcctgcatcc cgccgatgga gccgccgacc
     1861 accgcgcgca gacgctcgac gccgaggcgg tcgagcagcc gcgcctgcgc gcgcaccatg
     1921 tcggcgatgc tgaccagcgg gaagtccgcg ccgtacggct cgcccgtccg cgggttaatc
     1981 gaacccggcc ccgtcgagcc gtaacaagac ccgatgacgt tcacgccgat gacgcagtgg
     2041 cgagtcgaat cgaagacccc gcccgcgccg aacaactccg gccaccagtc tgccacgcgc
     2101 gccgaacccg acagcgcgtg gcagacgaga atcgcgttgt cgcgccgctc gttcagctcg
     2161 ccgtacatcg cgtagcgcag cgtcaagggc cgcagctcgc ccccgctcac acgaaacgcc
     2221 gcgtcacgcg cgaaagtaca gtcggcctcg atcgtcggtt cgatggttgt gctcataatt
     2281 caagtaggca gtaggcagta agcagtaggc agcaggcagt taaggaaaag cgcatttcaa
     2341 atttgatatt tgaaatgcca catcaaagaa ctcagtcccc gaatggtttt aactgcctac
     2401 tgcctgctgc ctactgccta ctgcttttca tcctgtgttc cgcaggcccg ccgagacgcc
     2461 gttgatggtc gcggcgagcg cgtagttgag ttcgtcgcgt tcttcgcccg cgcgcttgcg
     2521 gcggagcagc tcgacctgaa tcaggctgag cgaatcgacg taagggttgc gcagacggat
     2581 cgagcgggcg agcacctggt tgttttccag cagccgcgac tggcggctga cgcgcaggag
     2641 catccggcgc gtgcgccgga actcttcggt gacgaggtcg aagacgcgcc gccgcaaatc
     2701 ttcgtccgtg accagcccgg cgtagagcgc ggcgatggag aagtccgcct tcgccatgcc
     2761 gatctcgacg ttcgagatca aatcctcgaa gagcgggaag ccctcgaaca tctcttgcag
     2821 aaggcgctcg cgcgcggcgt ccgtgcccgc gaacttttcg agcgcgtggc cgacgccgaa
     2881 ccaagcgggc agcacgtggc ggctctgcat ccagccgaaa acccatggga tggcgcgcag
     2941 gtcttcgagg ccgcgccgct caccgcgacg gggcgggcgc gagccgatgc gcgcgtgttc
     3001 gagttccaag acgggcgtcg cctcttcgaa gtaagggagg atgtccgggt tctccgcgac
     3061 gttggcgcgg tagaagtcga aggcgtcgcg cgacatctct tcgagcgcgg cctcccactc
     3121 ccgcagttcg tcccgcgcgg gcgtgcccgc cgcacggctc agggcttcga gcgaggcggc
     3181 aaccatcagt tcgaggttgc gctcggcgag cacaggctcg gagtatttcc agttgagcac
     3241 ctcgccctgc tcggtgatct tgaactggcc gccgaaggcg ccggcgggct gcgcgacgat
     3301 ggcgcggtgg gtggggccgc cgccgcgccc caccgtgccg ccgcgcccgt ggaagaggcg
     3361 caggcgcacg tcgcactccg aggcgacgcg gtggagcgcg cggtgcgcct tgtaaatctc
     3421 ccacgtgctc gtgagcatcc cgccgtcctt gttcgagtcc gag
//
LOCUS       DBNGOH010001060         2065 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_893342, whole genome shotgun sequence.
ACCESSION   DBNGOH010001060 DBNGOH010000000
VERSION     DBNGOH010001060.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2065)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_893342"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1332
                     /locus_tag="AC159P_20675"
     CDS             <1..1332
                     /locus_tag="AC159P_20675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697916.1"
                     /translation="EEGFLKLVRDAGLRMSQPTRVEILSRVAAPQSAPPDTLRPARSK
                     TYAEAISLLRQAAERTAAKWEPILASSPPEQATRSGGLGFFTEGDNLTGEWKEQKGWF
                     WTGTFWVGTLWRLHGKTGDARFRRWAEQWNAHFLGKEHTQNHDTGFLNFYTSVLAYRQ
                     TKDAKYRDGALRAAERLKQLYNPQTELVAAWAVGGDDTIIDTMMNLQIWWWAARESGD
                     AQWRELGLKHALKSREWLIRPDGSVIQSVHYNPGDNRQEFFSSASGPTTAQVPNSSKP
                     GEKVFTHTHQGFAADTTWSRGAAWALYGYAVAYDETKDPRLLETSERIAAFVLDRLPE
                     DGVPWYDFDDEGVHFRNRDTSAAALVAGGLLRLSELTPDKTRAATYRREAERITQSLI
                     DRYLTPTSADDKTPPGVLRHGSSNRPADVPLVYGDYYLLETLLWLEDKKKS"
     gene            1367..>2065
                     /locus_tag="AC159P_20680"
     CDS             1367..>2065
                     /locus_tag="AC159P_20680"
                     /inference="COORDINATES: protein motif:HMM:NF016826.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HIR3697917.1"
                     /translation="MSTQMQESAERWVFRGTNRHKGRRLAVTPENSAMEHLAYGRIIL
                     DAEVSRAGFSTGGREVGLICLAGECALKAGGETHALARYDSIYLPRDTEVEITTESAV
                     DLAECSAEVEQRYPLQVVRYADVEGNGALSFKTGGPANTRRVNITLGKNVEAGRILAG
                     FTTSEPGHWTSWPPHEHAAMLEELYVYYDMPAPAFGVQFVYTNPDEPEFVGVVRDGDA
                     VVMPRGFHPNVSVPG"
BASE COUNT          365 a          709 c          690 g          301 t
ORIGIN      
        1 gaagaaggtt ttctgaaact cgtccgcgac gcgggcctcc ggatgtcgca gccgacgcgc
       61 gtggaaatct tgtcgcgggt agcggcgccg cagtccgccc cgcccgacac gctgcggccc
      121 gcgcgcagca agacgtacgc cgaggccatc tcgcttttgc gacaggccgc cgagcgcacc
      181 gccgcgaagt gggagccgat cctcgcctcg tcgccgcccg agcaggcgac gcgctcgggc
      241 ggcctcggct tcttcaccga aggcgacaac ctgacgggcg agtggaagga gcagaagggc
      301 tggttctgga cgggcacctt ctgggtcggc acgctctggc ggctccacgg gaagacgggc
      361 gacgcgcgct tccgccgttg ggccgaacag tggaacgcgc acttcctcgg caaggagcac
      421 acgcagaacc acgacacggg ctttctcaac ttctacacgt cggtgctggc ctacaggcag
      481 accaaagacg cgaagtatcg tgacggggcg ctgcgcgcgg cggagcgcct gaagcagctt
      541 tataatccgc agaccgaact cgtcgcggcg tgggccgtcg gcggcgacga caccatcatc
      601 gacacgatga tgaacctgca aatctggtgg tgggccgcgc gcgagtcggg cgacgcgcag
      661 tggcgcgagc tgggtctgaa gcacgcgctc aagtcgcgcg agtggctcat ccgcccggac
      721 ggctccgtca tccaatcggt tcattacaac cccggcgaca atcgtcagga gttcttctct
      781 tcggcctcgg ggccgacgac cgcgcaggtg ccgaacagct cgaagcccgg cgagaaggtt
      841 ttcacgcaca cgcatcaggg cttcgccgcc gataccacct ggtcgcgcgg cgcggcgtgg
      901 gcgctctacg gctacgccgt cgcctacgac gagacgaaag acccgcgcct gcttgagacc
      961 tcggaacgca tcgccgcctt cgtcctcgac cgcctgcccg aagacggcgt gccgtggtac
     1021 gacttcgacg acgagggcgt ccacttccgc aaccgcgaca cttcagccgc cgcgctcgtc
     1081 gcgggcggcc tgctgcggct ctccgagctg accccggaca agacgcgcgc cgccacctac
     1141 cggcgcgagg ccgagcgcat cacgcagtcg ctcatcgacc gctacctgac gcccacgagc
     1201 gccgacgaca agacgccgcc cggcgtcctg cgccacggtt cgagcaaccg cccggcggac
     1261 gtgccgctcg tctacggcga ttattacctg ctcgaaacgc tgctctggct cgaagacaaa
     1321 aagaagagct agccgcgata agttcgggca aaagaaaggt acaatcatga gcacgcagat
     1381 gcaggagtcg gcagagcgtt gggtcttccg cggcacgaac cggcacaagg ggcggcgcct
     1441 tgccgtgacg ccggagaaca gcgcgatgga gcacctcgcc tacgggcgca tcatcctcga
     1501 cgcggaggtg tcgcgcgccg ggttctccac gggcgggcgc gaggtcggct tgatttgcct
     1561 cgcgggcgag tgcgcgttga aggccggggg cgagacgcac gcgctcgcgc gttacgattc
     1621 gatttacctt ccgcgcgaca ccgaagtcga aatcacgacc gagagcgcgg ttgacctcgc
     1681 ggagtgctcg gcggaggtgg agcagcgtta ccccttacag gtcgtgcgct acgcggacgt
     1741 tgaagggaac ggcgcgctga gtttcaagac gggcggcccg gcgaacacgc gccgcgtcaa
     1801 catcacgctc ggcaagaacg tcgaggccgg tcgcatcctc gccggcttca ccacgtctga
     1861 gccgggccac tggacttcgt ggccgccgca cgagcacgcg gcgatgctcg aagagctgta
     1921 cgtctactac gacatgcccg cgcccgcctt cggcgtgcag ttcgtctaca cgaaccccga
     1981 cgagccggaa ttcgtcggcg tcgtgcgcga cggcgacgcg gtcgtcatgc cgcgcggctt
     2041 ccacccgaac gtctcggtgc ccggc
//
LOCUS       DBNGOH010001061         8830 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4376508, whole genome shotgun sequence.
ACCESSION   DBNGOH010001061 DBNGOH010000000
VERSION     DBNGOH010001061.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8830)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8830)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8830
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4376508"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..985
                     /locus_tag="AC159P_20685"
     CDS             <1..985
                     /locus_tag="AC159P_20685"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006273975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HIR3697918.1"
                     /translation="VMERAARRLKRGKRGMNRRSFIGGAGACLAALAAGRTAFAAGGE
                     SRRRRVAVTLDDFNVFDTPALSGEARNEAILGALGQYRLRAVMYVAAKFVDGTDRMRL
                     LRRWDERGHLIGNHTYSHPEYSRKTFEEFTRDIVRAEEVLKVLPRYRKLFRFPYLKEG
                     ATPEQRDRMRAFLKERGYRNGHVTVDASDWYVDQRLRDRLKQDPRADTAPYRDFYLAH
                     IRERALFYDDLAQKVLGRPVRHTLLLHHNVLNAMFLGDLLGMFKREGWQLLDADDAFK
                     DPVYKSEPQTAPAGESLIWALAKQTGKYENLLRYPGEDGEYEKPRMDALGL"
     gene            1191..2165
                     /locus_tag="AC159P_20690"
     CDS             1191..2165
                     /locus_tag="AC159P_20690"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HIR3697919.1"
                     /translation="MSKWKSCGAALALTLAVAVCAFAQPQAAAKAGQAKREVAVTFDD
                     LPAVGGGLENLRSITERLVASFKRNGVPVVGFVNEGKLYRAGEVDERIALLRAWADAG
                     YELGNHTFSHINMTRAPLADYRDDIVRGETVTSMLMREKGLRLRYFRHPFLWTGITPE
                     YKRGLDEFLAARNYTVAPVSIDNGDYIFADVYRKAKARGDAETAKRVADAYVPYMEVM
                     FDYFERQSTALLGRDIKHILLVHANELNADRFDELAAMMKRRGYAFVTLEEALKDPAY
                     KLPEAQVTGGMSWLHRWRIARGEKAAEREPSEPSWVAELFRAGQQQGN"
     gene            2187..3344
                     /locus_tag="AC159P_20695"
     CDS             2187..3344
                     /locus_tag="AC159P_20695"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010661725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HIR3697920.1"
                     /translation="MSVRNMKGVLVVLFALGCGPRPSSARRTQALVVTPQRQSFAQEK
                     TSAARTKVFPGRSWEKVARPEDVGWSSSKLEGAKAYSREVGSTAVMIVEGGRVVEEWG
                     ATARRINLFSVRKSFLSALYGVYVAEGKIDLASTLGELGIDDHPPRLNAVEKRATVRE
                     LLQARSGVYHAAAYEAPRMKALRPRRYSHAPGTFWYYNNWDFNALGTIFERQTRRKIG
                     DAFKERIAAPTGMEDFRAQDVWYEREPSSIHAAYPFRVSARDAARFGLLYLRGGEWQG
                     REVIPAAWVAESTRVYSVAGGRGGYGYLWWVAADGKHLPGVTIPDGSYSARGSGGQYI
                     LVIPEYDLVIVHRFDPATAKPEEEVTPDKFGPLVKLILDARPAGDARANSG"
     gene            complement(3472..5730)
                     /gene="clpA"
                     /locus_tag="AC159P_20700"
     CDS             complement(3472..5730)
                     /gene="clpA"
                     /locus_tag="AC159P_20700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014101416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HIR3697921.1"
                     /translation="MITKELQATLNVAAIEAIKRRHEYVTLEHLLYALLHEKTAGNIV
                     RQCGGDAEALVRDLEKFFEEKMKTVADTGEQMPEYTKAFQRVVEYALLQAEGSGQKDV
                     DGGNVLAALFQAEHSHAVYLLRKQGVTRLDVLNYMAHGISKLDAGDFLTEGGDDTEMD
                     AAAQAEQARDPLAAFTTNLVERAAEGKIDPLIGRESEVARTIQVLCRRRKNNPIYVGD
                     PGVGKTAIAEGLALKIQKGEVPDALKGAEVFALDMGALLAGTKYRGEFEQRLKAVIAA
                     LKKKPNCVLFIDEIHTIVGAGAVSGGSMDASNIIKPVLASGEIRCIGSTTYPEYKAAF
                     ERDRALARRFQKIEVGEPTVPETVQILAGLKTYYEEHHGVKYTDEALSAAAELSAKHV
                     HDRFLPDKAIDVIDEVGAMVKLLPAGERPAEIGVQEIELVVARMAKIPPRTVAGSERD
                     RLKNLDGELKSVIYGQDHAIAQMVNAIKLSRSGLGAPDKPIGSFLFSGPTGVGKTELA
                     KQLAVALGVAFLRFDMSEYMEPHTVSRLIGAPPGYVGFDQGGLLTDAVNKTPYAVLVL
                     DEIEKAHPNLFNILLQVMDHATLTDNNGKKADFHNVILIMTTNAGAREMSGEKIGFQK
                     QQGGLGLGRGRGAIERAFSPEFRNRLDAWIAFEHLSFETIERVVDKFVGEVRAQLSEK
                     NVRVELTPPARRWLAERGYDRQMGARPMARLIQEKIKAPLAEEILFGKLQSGGTVVVE
                     EKGGELDLIFNN"
     gene            complement(5856..6188)
                     /gene="clpS"
                     /locus_tag="AC159P_20705"
     CDS             complement(5856..6188)
                     /gene="clpS"
                     /locus_tag="AC159P_20705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015337108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HIR3697922.1"
                     /translation="MADWEPEYVFEEDVATESEERLKKPPLYRVLLHNDDFTTMEFVV
                     YVLETVFQKTDSDAVRIMLNVHNEGVGVAGVYTYEVAEMKCERVMQMARAQEYPLLCT
                     LEEAPESE"
     gene            complement(6341..7744)
                     /locus_tag="AC159P_20710"
     CDS             complement(6341..7744)
                     /locus_tag="AC159P_20710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3697923.1"
                     /translation="MRDSREGRAGGGEAEASGGRREAGGAREAGPQGAPPPTASRLPA
                     IFRALRHRNYRLFFGGQFVSLTGMWMQSVAQSWLVYRLTGSAVLLGFVSFSGQIPVFL
                     LAPVGGAVADRRDRHRIIIITQTTAMLLASTLAALTLAGRVEVWHLFVLAALLGVTNA
                     FDIPARQSFVVEMVGREDLVNAIALNSSMFNGARVVGPAVAGLLVAAIGEGWCFAANA
                     VSYVAVIAGLLAMNMERRRRAPARGSALSSIAEGFRFVARTGPVRALLLLLGLVSLTG
                     MPYAVLMPIFADRSLHGGPGGLGLLMGASGVGALAGALSLAARRGLRGLGRWVAFASA
                     GFGVSIMLFSVSRSFWLSAALLVPAGFSLMLQMASSNTLVQSMVPDALRGRVMAVYSM
                     MFMGMAPIGALLAGAVAQRVGAPLTVALGGAACVVGAVVFGLRLPAHRDAARQLIVAL
                     QMTAGDPAEEVTGDAIK"
     gene            7982..>8830
                     /locus_tag="AC159P_20715"
     CDS             7982..>8830
                     /locus_tag="AC159P_20715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697924.1"
                     /translation="MNHGYTYQSTLAASEGIRWRIEDIIGGDKRLDFTKPFMPEALAR
                     VEPLSFLTADEKLLLNQIRGNAYLCIFGLVEEFILPFVLDHARAELQPDDSRTRALLQ
                     FAGEEAKHIQLFKRFREEFESGFGFDCKVIGPGAEIARAILAHDPLAVALVTLHIEWM
                     VQPHYVEGMKDNGQLDPQFKSLLRHHWMEEVQHAKLDTLMVAALAEGRTESEILAAVE
                     EYLEIGGFIDEGLAGQVELDLDAFTRATGRELSDEEKAEFRRVQLQANRWTYLGSGMT
                     HERVLAT"
BASE COUNT         1512 a         3011 c         2910 g         1397 t
ORIGIN      
        1 agtcatggag cgcgccgcgc gccgcttgaa aagggggaag cgcgggatga atcgaaggag
       61 ctttatcggc ggcgcgggcg cgtgcctcgc cgccctcgcc gcgggccgga cagcattcgc
      121 ggcgggcggc gagagccgcc gccgccgcgt cgccgtcacg ctcgacgact tcaacgtctt
      181 cgacacgccc gcgctctcgg gcgaggcccg caacgaggcg atcctcggcg cgctcggcca
      241 gtacaggctg cgggccgtga tgtacgtcgc cgccaagttc gtggacggca cagaccgcat
      301 gcgcctgctc cgccgctggg acgagcgcgg ccacctcatc ggcaaccaca cctactcgca
      361 cccggaatat tcgcggaaaa cattcgaaga gttcacgcgc gacatcgtgc gcgccgaaga
      421 ggtgctgaag gtgctcccgc gctaccgcaa gctcttccgc ttcccgtacc tcaaagaagg
      481 cgccacgccc gaacagcgcg accgcatgcg cgcgttcctc aaggagcgcg gctaccgcaa
      541 cgggcacgtg acggtggacg cctcggactg gtacgtcgat cagcgtctgc gcgaccgcct
      601 caagcaagac ccgcgcgccg acacggcgcc ttatcgagac ttctacctcg cacacatccg
      661 cgagcgcgct ctcttctacg acgacctcgc gcagaaggtg ctcgggcgcc ccgttcgtca
      721 cacgctgctg ctgcaccaca acgtcctgaa cgcgatgttc ctcggcgacc tgctcgggat
      781 gttcaagcgc gagggctggc aactgctcga cgcggacgac gccttcaaag accccgtcta
      841 caagtccgag ccgcagacgg cgcccgcggg cgagagtctg atctgggcgc tggcgaagca
      901 gaccggcaaa tacgaaaacc tactgcgcta tcccggcgaa gacggcgagt acgaaaagcc
      961 gcgcatggac gcgctcggac tgtagcgcgt cccccggagc aagcttcagg cgtgtgcggt
     1021 acgtcgtgaa tcctgtaggg gcagggcttg tccctgcccg cggtcaaatt tcattcaacg
     1081 tcgaatcaac cggacgttga ggggcaggga caagccctgc ccctacagtt gtggcgagtg
     1141 tgttacggtc tacacaaaaa ctcggcaatg aaggagagac cctcgtgacc atgtcgaagt
     1201 ggaagtcgtg cggcgcggct ctggcgctga cgcttgccgt ggccgtgtgc gcgttcgcac
     1261 agccgcaggc ggcggcaaaa gcggggcagg cgaaaaggga agtggccgtc accttcgacg
     1321 atttgccggc ggtcggcggc ggcctcgaaa acctgcgctc gatcacggag cgcctcgtcg
     1381 cgagcttcaa gcgcaacggc gtgccggtcg tcggcttcgt caacgagggc aagctctacc
     1441 gcgcgggcga ggtggacgag cgcatcgcgc tgctgcgcgc gtgggccgac gcgggttacg
     1501 aactcggcaa ccacaccttc tcgcacatca acatgacccg cgcgccgctc gccgactacc
     1561 gcgacgacat cgtccgcggc gagaccgtga cctcgatgct catgcgcgag aaggggctgc
     1621 gcctgcgcta cttccgccac ccgttcctct ggacgggcat cacgcccgag tacaagcgcg
     1681 gcctcgacga attcctcgcc gcgcgcaact acacggtcgc gcccgtctcg atcgacaacg
     1741 gcgactacat cttcgcggac gtgtaccgca aggccaaagc gcgcggcgac gcggagaccg
     1801 ccaaacgcgt cgccgacgcc tacgtgcctt acatggaggt gatgttcgac tacttcgagc
     1861 ggcagtcaac ggcgcttctc gggcgcgaca tcaagcacat cctcctcgtc cacgccaacg
     1921 agctgaacgc cgaccgcttc gacgagctgg ccgcgatgat gaagcggcgc ggctacgcct
     1981 tcgtcacgct cgaagaggcg ctgaaagacc ccgcctacaa gctgcccgag gcgcaggtca
     2041 cgggcggcat gtcgtggctc caccgctggc gcatcgcgcg cggcgagaag gccgccgagc
     2101 gcgagccgtc agagccttcg tgggtcgcgg agctgttccg cgcgggccag cagcagggca
     2161 attgaggttc gacgcgggga gggaaggtga gcgtcaggaa catgaaggga gtgctggtcg
     2221 tcctcttcgc gctcggctgc gggccgcggc cttcgtcagc gcggcgcacg caggccctcg
     2281 tcgtcacgcc tcagcgacag tcgttcgcgc aggagaaaac atcagccgcg aggacgaagg
     2341 tcttccccgg caggagttgg gagaaggtcg cgcggcccga ggatgtcggc tggtcttcgt
     2401 cgaagctcga aggagcgaag gcgtactcgc gcgaggtcgg ctcgaccgca gtgatgatcg
     2461 tcgagggcgg gcgcgtcgtc gaagagtggg gcgcgaccgc gcggcgcatc aacctcttct
     2521 ccgtccgcaa gagtttcctg agcgcgctct acggcgtcta cgtggccgaa ggcaagatcg
     2581 acctcgcgag cacgctcggc gaactcggca tcgacgacca tccgccgcgg ctcaacgccg
     2641 tggagaagcg cgccaccgtg cgcgagctgc tgcaagcgcg ctcgggcgtc taccacgcgg
     2701 cggcctacga ggcgccgcgc atgaaggcgc tccgccccag gcgctacagc cacgcgcccg
     2761 gcaccttctg gtattacaac aactgggact tcaacgcgct cggcaccatc ttcgagcgac
     2821 agacgcggcg caaaatcggc gacgccttca aggagcgcat cgccgcgccc acggggatgg
     2881 aagacttccg cgcgcaggac gtgtggtacg agcgcgagcc ttcttcgatt cacgcggcct
     2941 accctttccg cgtctcggcg cgcgacgcgg cgcgcttcgg gctgctgtac ctgcgcggcg
     3001 gcgagtggca agggcgcgag gtcatccccg ccgcgtgggt cgcggagagc acgcgagtct
     3061 actcggtcgc cggaggccgc ggcggctacg gctacctctg gtgggtcgcc gccgacggca
     3121 agcacctgcc cggtgtgacc atccccgacg gctcatactc cgcgcgcggc tccggcggcc
     3181 agtacatcct cgtcatcccc gaatacgatc tcgtcatcgt ccaccgcttc gaccccgcga
     3241 ccgcgaagcc cgaagaggag gtcacgcccg acaagttcgg cccgctcgtc aagctcatcc
     3301 tcgacgcgcg gcccgcgggc gacgcgcgcg cgaactctgg ctgagagtac cgggcgttta
     3361 tctcaacaca gagggcacgg agaagaaata tggctttctc tgtgctccct gtgaatcctc
     3421 tgcgaactct gtgttgaaat gaacccgcag tggactcaac cgcaatccgc cctagttatt
     3481 gaatatcaag tcaagctcgc cgcccttctc ttcaaccacg accgtcccgc ccgactgtaa
     3541 tttgccgaag aggatttcct cggcgagcgg cgctttgatc ttctcctgaa tcaggcgggc
     3601 catgggccgc gcgcccatct ggcggtcgta gccgcgttcg gcgagccagc ggcgcgcggg
     3661 cggggtcagc tcgacgcgga cgttcttctc ggagagctgc gcgcggactt cgccgacgaa
     3721 cttatcgacg acgcgctcga tggtctcgaa cgagaggtgc tcgaaggcga tccacgcgtc
     3781 gaggcggttg cggaactcgg ggctgaaggc gcgctcgatg gcgccgcgcc cgcggcccaa
     3841 accgaggccg ccctgctgct tctggaagcc gatcttctcg cccgacatct cgcgcgcgcc
     3901 cgcgttggtc gtcatgatga ggatgacgtt gtggaagtcg gccttcttgc cgttgttgtc
     3961 ggtgagcgtg gcgtgatcca tcacctgcaa gaggatgttg aagaggttcg ggtgcgcctt
     4021 ctcgatctcg tcgaggacga gcacggcgta cggcgtcttg ttgaccgcgt ccgtcagcag
     4081 ccccccctgg tcgaagccga cgtagccggg cggcgcgccg atgaggcgcg agaccgtgtg
     4141 cggctccatg tactcggaca tgtcgaagcg caggaaggcg acgcccaaag cgacggcgag
     4201 ctgcttggcc agctccgtct tgccgacgcc cgtcgggccg gagaagagga acgagccgat
     4261 gggcttgtcg ggcgcgccga ggccggagcg cgagagcttg atcgcgttca ccatctgcgc
     4321 gatggcgtga tcctgcccgt agatgaccga cttcagctcc ccgtcgaggt tcttgaggcg
     4381 gtcgcgctcc gagcctgcga cggtgcgcgg cggaatcttc gccatgcgcg cgacgaccaa
     4441 ctcgatctcc tggacgccga tctcggcggg gcgctcgcct gcgggcagca gcttgaccat
     4501 cgcgccgact tcgtcgatca cgtcgatggc cttgtccggc aggaagcggt cgtggacgtg
     4561 cttggcggaa agctctgcgg cggcgctcaa agcctcgtcc gtgtacttca cgccgtggtg
     4621 ctcttcgtag taggtcttca gcccggcaag aatctggacg gtctcgggca cggtcggctc
     4681 gccgacctcg atcttctgga agcggcgcgc gagcgcgcgg tcgcgctcga aggcggcctt
     4741 gtattcgggg taggtggtcg agccgatgca gcgtatctcg cccgaggcga gcaccggctt
     4801 gatgatgttc gaggcgtcca tcgagccgcc cgagaccgcg cccgcgccga cgatggtgtg
     4861 aatctcgtcg atgaagagga cgcagttcgg cttcttcttg agcgcggcga tgacggcctt
     4921 caagcgctgc tcgaactcgc cgcgatactt cgtcccggcg agcaaagcgc ccatgtcgag
     4981 tgcgaagacc tcggcgccct tcagggcgtc gggcacctcc cctttctgaa tcttcagcgc
     5041 cagcccctcg gcaatcgcgg tcttgccgac gccggggtcg ccgacgtaga tcgggttgtt
     5101 cttgcggcgg cggcagagca cctgaatcgt gcgcgcgacc tccgattcgc gcccgatgag
     5161 cgggtcgatc ttgccctcgg cggcgcgctc gacgaggttc gtcgtaaagg ccgcgagcgg
     5221 gtcgcgcgcc tgctcggctt gcgcagcggc gtccatctcg gtgtcgtcgc cgccttcggt
     5281 gaggaagtcg cccgcgtcga gcttcgagat gccgtgcgcc atgtagttca gcacgtcgag
     5341 gcgcgtcacg ccctgcttgc gcaaaagata gacggcgtgc gagtgctcgg cctgaaagag
     5401 cgcggccagc acgttgccgc cgtccacgtc cttctgcccc gacccttcgg cctgaaggag
     5461 cgcgtactcg acgacgcgct ggaacgcctt cgtgtactcg ggcatctgct cgcccgtgtc
     5521 ggccacggtc ttcatcttct cttcgaagaa cttttcgagg tcgcgcacca gcgcctcggc
     5581 gtcgccgccg cactggcgga cgatgttgcc cgccgtcttc tcgtgcaaga gcgcgtacaa
     5641 caagtgttcc agcgtcacgt actcgtggcg ccgcttgatc gcctcaatcg cggcgacgtt
     5701 gagcgtggct tgtaattcct tcgttatcat ctaagtgaac ggtgaatagc gaatggtcaa
     5761 tggtaaatag taagtcgctt ggatgcagac cgcttcggtt tcattgctcg cgtcgtgaac
     5821 cgctcagcca ctattcacca tttaccattt accattcact cactttcggg tgcttcttcc
     5881 agcgtgcaga gcagcgggta ctcctgcgcg cgggccatct gcatgacgcg ctcgcacttc
     5941 atctcggcga cctcgtaggt gtagacgcca gccacgccga cgccctcgtt gtggacgttg
     6001 agcatgatgc gcaccgcgtc ggagtcggtc ttctgaaaga cggtttcgag cacgtacacc
     6061 acgaactcca tcgtcgtgaa gtcgtcgttg tgcagcagga cgcggtagag cggcggcttc
     6121 ttcagccgct cctcgctctc ggtcgccacg tcctcctcga acacgtactc cggctcccag
     6181 tccgccatgc gtgaatctta aagggaaaga ggggaaaagg cgaaaggggg aagaggggaa
     6241 aagggggaaa gggaaaaagg ttaaaagtgg aagaggaagc aggttgaatg agccgtgtgc
     6301 tcaaccgtct tccttttcgc cctttcccct tttccccttc ttacttgatg gcgtcgcccg
     6361 tcacctcttc cgccgggtcg ccggcggtca tctgaagcgc gacgatgagt tggcgcgcgg
     6421 cgtccctgtg cgcgggcagg cgcagcccga agacgacggc gccgacgacg caggccgcgc
     6481 cgccgagcgc gaccgtcagc ggcgcgccga cgcgctgcgc gaccgcgccc gccaataaag
     6541 cgccgatggg ggccatgccc atgaacatca tcgagtagac ggccatcacg cgcccgcgca
     6601 aggcgtccgg caccatcgac tgcacgaggg tgttcgacga ggccatctgc aacatcagcg
     6661 agaaccccgc cggcacgagc agcgccgccg acagccagaa cgagcgcgac acggagaaga
     6721 gcatgatgct cacgccgaag cccgccgagg cgaacgcgac ccagcgcccg agtccgcgca
     6781 aaccgcgccg cgccgccaga ctcagagcgc ccgcgagcgc cccgacgccc gacgcgccca
     6841 tcagcagccc cagcccgccg gggccgccgt gcagactccg gtcggcgaag atcggcatca
     6901 gcacggcgta gggcatcccg gtcagactca ccagcccgag cagcagcagc agcgcgcgta
     6961 cggggccagt gcgcgcgacg aagcggaacc cctcggcgat gctggagagc gccgagccgc
     7021 gcgcgggcgc gcgccgccgc cgctccatgt tcatcgcgag cagcccggcg atgacggcga
     7081 cgtagctcac ggcgttggcc gcgaagcacc agccctcgcc gatggccgcc acgagcagcc
     7141 ccgccaccgc cggcccgacg acgcgcgcgc cgttgaacat cgaggagttg agcgcgatgg
     7201 cgttgaccaa atcctcgcgc ccgaccatct cgaccacgaa cgactgccgc gcggggatgt
     7261 cgaacgcgtt cgtcacgccg agcagcgccg cgagcacgaa gaggtgccag acctcgaccc
     7321 ggcccgcgag cgtcagcgcg gcgagcgtgg aggcgagcag catcgccgtg gtctgcgtga
     7381 tgatgatgat gcggtggcgg tcgcggcggt cggcgacggc gccgcccacg ggcgcgagca
     7441 ggaagaccgg aatctgcccg gagaagctga cgaacccgag cagcacggcg gagccggtca
     7501 ggcggtagac gagccagctc tgcgccaccg actgcatcca catgcccgtc agcgagacga
     7561 actgcccgcc gaagaagagt cggtagttgc ggtgccgcag cgcgcggaag atggccggca
     7621 gccgcgacgc cgtgggcggc ggcgcccctt gcggccccgc ttcccgcgcc cctccggcct
     7681 ctcgccgtcc gccgctcgcc tccgcctcgc ccccgcccgc tcgcccctcc cgcgaatctc
     7741 tcatcaacgt gccccctcgc ccgcccgccg cccgccgtcg cctgtccgaa gtcgtttccg
     7801 gccagtgtaa ccaactccgt cttcatcctc ctaccagttc gcggcggagg cgcgcgccgc
     7861 ggcgaaaatt atcgaaaaaa atctcgcggt cgtgatccgc cgccggcctt tcctccgcac
     7921 gtgtgtgtga aggcggcgat gggcgccgca gactttcggc aagcgagaag gaggagcttt
     7981 catgaaccac gggtacacgt accaatcgac gctggccgcg tccgagggca tccgctggcg
     8041 gatcgaggac atcatcggcg gcgacaagcg gctcgatttc acgaagccgt tcatgcccga
     8101 ggcgctcgcg cgcgtcgagc cgctctcgtt cctgacggcg gacgaaaagc tgttgctcaa
     8161 ccagatcagg gggaacgcct acctgtgcat cttcgggctg gtcgaggagt tcatcctccc
     8221 cttcgtgctg gatcacgcgc gcgccgaact ccagccggac gacagccgca cccgcgcgct
     8281 gctccagttc gccggcgaag aggccaagca catccagctc ttcaagcgct tccgcgagga
     8341 gttcgagagc ggcttcggct tcgactgcaa ggtcatcggg ccgggcgccg agatcgcccg
     8401 cgccatcctc gcgcacgacc cgctcgccgt ggcgctcgtc accctccaca tcgagtggat
     8461 ggtgcagccc cactacgtcg agggcatgaa ggacaacggg cagctcgacc cgcagttcaa
     8521 gagcctgctc cgccaccact ggatggagga ggtgcagcac gccaagctcg acacgctgat
     8581 ggtcgcggcg ctggccgagg ggcgcaccga gtcggagata ctcgcggcgg tcgaggagta
     8641 cctcgagatc ggcggcttca tcgacgaggg gctcgccggg caggtcgagc tcgacctcga
     8701 cgccttcacg cgggcgaccg gccgcgagtt gagcgacgag gagaaggccg agttccgccg
     8761 ggtgcagctc caggcgaacc gctggaccta tctcggctcg ggtatgaccc acgagcgggt
     8821 tctcgcgacg
//
LOCUS       DBNGOH010001062         2230 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4560104, whole genome shotgun sequence.
ACCESSION   DBNGOH010001062 DBNGOH010000000
VERSION     DBNGOH010001062.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2230)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2230)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2230
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4560104"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..752)
                     /locus_tag="AC159P_20720"
     CDS             complement(<1..752)
                     /locus_tag="AC159P_20720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697925.1"
                     /translation="MRSSRMQFAVLHSQPVGARRLLRPLACVAVVAACALLFFTRGAS
                     AVNRTWDGGGATNNWSEDANWSDDTEPVAGDIAVFDGTSTKNATIDVSINVAAIYINP
                     GYTGTPGVAGTITQAAGVAVTLTGGPLRTCTDPNRQNFFAFCQEGGTFNGGNAALDFD
                     GAFGLGGGAFTASSGTTFFGAGFTHIAGGTFDHNNGTAIFDGAGDTINVGSGNPPAPP
                     ETFNNLTFTRSNNTVSFLEGTLVVVGALALTDG"
     gene            complement(788..1096)
                     /locus_tag="AC159P_20725"
     CDS             complement(788..1096)
                     /locus_tag="AC159P_20725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697926.1"
                     /translation="MRRRKRTEVFSETEVEIEVRRRTRRLAPVWCAACGAEVEMVPPD
                     VAAAVSEVSARTVYGWVERGRVHFTETAEGALLVCLGSLPAALGEAEDARGLLTDGAE
                     "
     gene            complement(1153..>2230)
                     /locus_tag="AC159P_20730"
     CDS             complement(1153..>2230)
                     /locus_tag="AC159P_20730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697927.1"
                     /translation="AANNPKNQSARVLLDWFTDQFEKDWPRIRFGGTGEGATNGFLIQ
                     GPNDSTKLRIMNDGRVSIGPSSQAELLVTRTSVGNNERGLVVAVEGTLGQGEAAFFSN
                     TATDRADVLVLRVGTETPGDQDDFIKFQQGTGSPVGAIQGNGSGGIHLNSGAADYAEY
                     LPRLDPAEKIEPGEIVGLFGGRVTKKTRGAGQVMAVSTGPIILGNDPGEKARAEYEKV
                     AFIGQVAVRVRGAVRAGDLIVASGRDDGTGIAVSPERITPEQFEQAVGEAWESSADAG
                     VKPVRTAVGLIQRDPTVRRLVAANRRQARQLAALGAQLAALESRMNRELAALKSITSH
                     QKTGASRRATVRRQSAGNARGAGR"
BASE COUNT          302 a          763 c          751 g          414 t
ORIGIN      
        1 ccgtccgtga gggcgagcgc gccgacgacg acgagcgtgc cttcaaggaa ggagaccgtg
       61 ttgttgctgc gtgtgaaggt gaggttgttg aaggtctcgg gcggggcggg cgggttcccg
      121 ctgccgacgt tgatggtgtc gcccgcgccg tcgaagattg ccgtcccgtt gttgtggtcg
      181 aacgtgccgc cggcgatgtg cgtgaagccg gcgccgaaga aggtggtgcc gctcgacgcc
      241 gtgaaggcgc cgccgccgag gccgaacgcc ccgtcgaagt cgagcgccgc gttgccgccg
      301 ttgaacgtgc cgccctcctg acaaaaggcg aagaagttct gccggttcgg gtcggtgcag
      361 gtgcgcagcg gcccgccggt cagtgtgacg gccacgcccg ccgcctgcgt gatggtgccg
      421 gctacaccgg gcgtgccggt gtagccgggg ttgatgtaga tcgcggcgac gttgatgctc
      481 acgtcaatcg tcgcgttctt ggtgctcgtg ccgtcgaaga cggcgatgtc tcccgcgacg
      541 ggttcggtgt cgtcgctcca gttggcgtcc tccgaccagt tgttggtcgc gccgccgccg
      601 tcccacgtcc ggttgacggc cgacgcgccg cgcgtgaaga agaggagcgc gcacgcggcg
      661 acgactgcca cgcacgcgag cggacgcagg aggcggcgcg cgccgaccgg ctgactgtgc
      721 aaaacggcga attgcatcct gctgcttctc atgtgggcga cctccttctc ctgtgcgctg
      781 atgtttctca ctccgcgccg tctgtgagga ggccgcgcgc gtcttccgct tcgccgagcg
      841 cggcgggcag cgagccgagg cagacgagga gcgcgccctc cgccgtctcg gtgaagtgga
      901 cgcggccccg ctcgacccag ccgtagacgg tgcgcgcgct cacctccgaa acggcagcgg
      961 ccacgtccgg aggcaccatc tcgacttcgg cgccgcacgc cgcgcaccag accggcgcga
     1021 ggcgtcgcgt ccggcgcctg acctcgatct cgacctccgt ctccgagaag acctccgtcc
     1081 gcttccttct tctcatcccg tctcccgtac cggcccggaa gtatcggccc gcggccaatg
     1141 tcaggcgcga cctcatcggc ccgcgcctcg ggcgttgccg gcgctctgcc tacgcacagt
     1201 tgcgcgacga ctcgcacctg ttttctgatg gctggtgatg gacttgagcg cggcgagttc
     1261 cctgttcatt cttgattcga gggccgcgag ctgggcgccc agcgcggcaa gctgcctcgc
     1321 ctgtcggcgg ttggctgcga cgaggcggcg caccgtcggg tcgcgttgaa tcaacccgac
     1381 ggctgtgcgc accggcttca ctccggcgtc ggcggacgac tcccacgcct cccccaccgc
     1441 ctgctcgaac tgctccgggg tgatgcgttc gggcgagact gcaatgccgg tgccgtcgtc
     1501 gcggcccgaa gcgacgatga ggtcgccggc tcgcaccgcg ccgcgcacgc gcaccgcgac
     1561 ctgcccgatg aaagccactt tctcgtactc ggcccgcgcc ttttcgccgg ggtcgttgcc
     1621 gaggatgatc gggccggtgc tgacggccat cacctgtccc gcgccgcgcg tctttttcgt
     1681 cacgcggcca ccgaagaggc cgacgatttc gccgggttcg atcttctcgg cagggtcgag
     1741 ccgcggtaag tattccgcat agtcggcggc gccgctgttg aggtggatac cgcccgagcc
     1801 gtttccctga atcgcgccga cggggctgcc cgtcccctgc tggaacttga tgaaatcgtc
     1861 ttggtcgccc ggtgtttccg ttccgacccg gagcacaagc acgtcggccc tatctgtcgc
     1921 ggtgttcgag aagaaagccg cctcaccttg acctaaggtg ccctcgaccg ccacgactaa
     1981 tcccctctcg ttattgccca cgctggttct agtgacaagc aattccgctt gggacgacgg
     2041 cccgatgctg acgcggccgt cgttcatgat tcgcagcttc gtgctgtcgt tcggcccctg
     2101 tatgagaaaa ccgttggtcg cgccttcgcc ggtgccgccg aagcggatgc gcggccagtc
     2161 cttctcaaat tgatcggtga accagtccag aaggactctg gcgctctggt tcttcgggtt
     2221 gttggccgcg
//
LOCUS       DBNGOH010001063         6112 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4926134, whole genome shotgun sequence.
ACCESSION   DBNGOH010001063 DBNGOH010000000
VERSION     DBNGOH010001063.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6112)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6112)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6112
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4926134"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..477
                     /locus_tag="AC159P_20735"
     CDS             <1..477
                     /locus_tag="AC159P_20735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HIR3697928.1"
                     /translation="KAVIPGGSSVYIMPASDILGKDVRMDYEGLVAAGSSVGSGGFIV
                     MDDTVDVFESTKNLTEFYKHESCGWCTPCREGTDWLVKVFKRMAGGEGRPDDAQLLLD
                     LVDNIEGKSFCPLGDAASWPIQSAIKKFPEDFRRRMNAAAGNGASNKGGKDALPTT"
     gene            509..928
                     /locus_tag="AC159P_20740"
     CDS             509..928
                     /locus_tag="AC159P_20740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697929.1"
                     /translation="MRLALELILALGLAHAATPHLVAASPSAAPNVAAQCRRADTLIP
                     KTIRFARGRTTAVINDTVRLCTSHEYRLRARAGQTMSVHLATGPKTSFTVLPPTTELE
                     GADGVKDWQGELPDTGEYTITIGTDATAKYTLEVTIR"
     gene            965..1147
                     /locus_tag="AC159P_20745"
     CDS             965..1147
                     /locus_tag="AC159P_20745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008275722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HIR3697930.1"
                     /translation="MVFNVTIDRDEDGVWVVECPSIPGCVNQGVTKEEALENVKDAIR
                     LCLQVRVEKGYPRTMF"
     gene            1172..3463
                     /locus_tag="AC159P_20750"
     CDS             1172..3463
                     /locus_tag="AC159P_20750"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012602.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit G"
                     /protein_id="HIR3697931.1"
                     /translation="MSELVKVTINSQEFQVEKGARLIDVCREKGFAIPSFCYYADLAL
                     QASCRMCLVRIEKMPKLQTSCTITCTDGMVVTTESEEIEKAQRGMVEFLLANHPLDCP
                     VCDRGGECELQEMVFDWGGLEERFTERKNYYPEKYLSPIVANDPQRCILCKRCTRVCD
                     EWMGEDAIEAGGRGSHTVIGTYGGWLNCSQCGNCIEVCPTGTLLDATYRHQTRPWELA
                     RTTSTCTFCSDGCQLSIGSRAGEAMRVVARDRYVNGHNGEFLCIKGRFGHPFVNHEER
                     LKTPLIRYKKGGRLIPATWDDAIKFTAQRLQEIKDAHGGNSIGVVGSPRITNESNFAL
                     LKFAREVLETENYTATEAFSLAPFFENLGAPLATHRDIRHAKTIVLIGGEPEENQPLT
                     GRQIRQAVRNGGAKLVVVNSVPIRLKEQAAQFIHILPGTEDAAALAFTLQDSDGLAAR
                     KMGVEAGEIESALHLVNDTQGDVVIVFGGELSAEAQAVLAQAPHNFGEREGRRIFFHP
                     LPLYNNSVGVHDIGLMGGALNPLDILDAAGTTLSAMYVAGSFLPAHLEGREDALSKLD
                     FLVVQELFQTATTEQADVVLPAASFAEVDGTFTNSGGLVQRVRQSIPPVHQSKPDWVI
                     TSSIASAMGADFGYQMASSAVFLDIARNVAAYEGLRYPLLKDETNPRQAAHALAGRRD
                     VSRQLEDLRAIVNELEEGGEKVTGTPPVGHELFKIGTLTDKVPQFHLLAAGNPEPESV
                     LISPLYQITVDPPLRRGEAVAGD"
     gene            3593..4651
                     /gene="nuoH"
                     /locus_tag="AC159P_20755"
     CDS             3593..4651
                     /gene="nuoH"
                     /locus_tag="AC159P_20755"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013568996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HIR3697932.1"
                     /translation="MDWLNDTLGPANVRGLGQLIGINVAFVAVMLVVAYTVLAERRVL
                     AFIQGRLGPNRVGPGGVLQPFADLLKFIFKEEITPAKATRFVYFLAPMIAVIAAFMPI
                     IVYPFGPTVYLPFLGDVSLVIARLDIGLLFVLGITSVGVYGIMLAGWASNSKYSLMGG
                     LRASAQMISYELSLGLSLVGVVLLSGTLDLFNVVEQQSGWFGMKWNILFQPVGFIVYL
                     ISAIAETNRVPFDLPEAETELVAGFHTEYSALKFALFFMAEYVNMFTVSMLATTLFLG
                     GWNGPFVEQVPYLGPLYFFGKVFFFLFLYIWLRGTLPRFRFDQLMNFGWKFLLPVAVL
                     NIIVTATVAFFWYGAQTP"
     gene            4736..5479
                     /locus_tag="AC159P_20760"
     CDS             4736..5479
                     /locus_tag="AC159P_20760"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012709.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HIR3697933.1"
                     /translation="MLTILLFIIFGGLAVGCAVAVVAQRNPLYSAISLVGVFVALACL
                     YMTLAAPFIAAVQVIVYAGAIMVLVVFVIMLLNVEEEVRRPVRLRYLIPVAVALAAIL
                     VAETAFMLTLGGGPGAHRFDPASIPQTGVGLTASVGTGLFTEYLLPFEVTSVLILMAL
                     VGAITLARRTLAPAVTAPDYALGPTDFSIGDDNVPRTLPDAATPAEAVRHGDTETTGR
                     EMARLQIEQEEAERARDRKAKTEEEGGQR"
     gene            5476..5778
                     /gene="nuoK"
                     /locus_tag="AC159P_20765"
     CDS             5476..5778
                     /gene="nuoK"
                     /locus_tag="AC159P_20765"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012532342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HIR3697934.1"
                     /translation="MIPLSWYLALSAVLFTIGVTGVIIKRNAIQMFMCIELMLNAVNL
                     TFVAFASYFGDVTGHLFVFIVMTVAAAEAAVGLGIIIAIFRNRESLDVDEANILKL"
     gene            5876..>6112
                     /locus_tag="AC159P_20770"
     CDS             5876..>6112
                     /locus_tag="AC159P_20770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HIR3697935.1"
                     /translation="MIRYIIFAPLVGAVINWLVGRRVRSERFVGLVACGSVAVSAAFA
                     FWLAFGPEGALRGEEPRVILDHLWTWIQVGGFRAD"
BASE COUNT         1053 a         2057 c         1934 g         1068 t
ORIGIN      
        1 aaggccgtca tccccggcgg ctcctccgtc tacatcatgc cggcctccga catcctgggc
       61 aaagacgtgc ggatggacta cgaggggctc gtcgcggccg gctcctcggt cggctccggc
      121 ggcttcatcg tgatggacga cacggtggac gtgttcgagt ccaccaagaa cctgaccgag
      181 ttctacaaac acgagtcgtg cggctggtgc acgccctgcc gcgaaggcac cgactggctc
      241 gtcaaggtct tcaagcgcat ggccggtggc gagggccgcc ccgacgacgc gcagcttctg
      301 ctcgacctcg tggacaacat cgaaggcaag agcttctgcc cgctcggcga cgccgcctcg
      361 tggcccatcc agtcggcgat caagaagttc cccgaagact tccgccgccg catgaacgcc
      421 gccgcgggca acggcgcgag caacaagggc ggcaaggacg ccctgccgac gacctgagct
      481 tgagacgttc aacgtttggg aaagtacgat gagactcgca ctcgaattga ttctggccct
      541 cggtctcgcg cacgctgcga cgccgcacct tgtagcggca tcgccttcgg cggcgcctaa
      601 cgtcgcggct caatgcaggc gggccgacac cctcatcccg aagaccatcc gcttcgcgcg
      661 cggcaggacg accgccgtca tcaacgacac cgtccgcctc tgcacgagcc acgagtacag
      721 gctccgcgcc agagcgggcc agacgatgag cgtccacctc gccaccgggc cgaagacctc
      781 cttcacagtc ctgccgccga cgaccgaact cgaaggcgcc gacggcgtca aggactggca
      841 gggcgagctg cccgacacag gcgagtacac catcaccatc ggcaccgacg ccaccgccaa
      901 gtacacgctg gaagtgacga tcaggtagcc gcctgtcgtt tgcagcaggg gcgaggaaac
      961 agcaatggtc tttaacgtca ccatcgaccg cgatgaagac ggcgtctggg tcgtcgaatg
     1021 cccttcgatt ccggggtgcg tcaatcaggg agtgacgaaa gaggaagcgc tcgaaaacgt
     1081 caaggacgcg attcgcctct gcctgcaagt gcgcgtcgaa aagggctatc cccgaacgat
     1141 gttttaaacg cagaacactt tgtaattcaa aatgtcagaa ctcgtaaaag tcaccatcaa
     1201 cagtcaggag ttccaggtcg agaagggcgc gcggctgatc gacgtgtgcc gcgagaaggg
     1261 gttcgcgatc ccctccttct gctactacgc agacctcgcg ctccaggctt cgtgccggat
     1321 gtgcctggtg cgcatcgaga agatgccgaa gctccagacc tcttgcacga tcacctgcac
     1381 ggacgggatg gtcgtcacga ccgagagtga ggagatcgag aaggcgcagc gcgggatggt
     1441 cgagttcctg ctggcgaacc acccgctcga ctgcccggtc tgcgaccgcg gcggcgagtg
     1501 cgagttgcag gagatggtct tcgactgggg cggcctcgaa gagcgcttca ccgagcgcaa
     1561 gaactactac ccggagaaat atctttcgcc catcgtcgcc aacgacccgc agcggtgcat
     1621 cctgtgcaag cggtgcacgc gcgtctgcga cgagtggatg ggcgaggacg ccatcgaggc
     1681 gggcgggcgc ggctcgcaca ccgtcatcgg cacctacggc ggctggctca actgctcgca
     1741 gtgcggcaac tgcatcgagg tctgccccac ggggacgctc ttggacgcga cctaccgcca
     1801 ccagacgcgc ccgtgggagc tggcgcgcac cacctcgacc tgcacgttct gctcggacgg
     1861 gtgccagctc tccatcggct cgcgcgcggg cgaggcgatg cgcgtcgtcg cgcgcgaccg
     1921 ctatgtcaac ggccacaacg gcgagttcct ctgcatcaag ggccgcttcg ggcacccgtt
     1981 cgtcaatcac gaagagcggc tgaagacgcc gctcatccgc tacaagaagg gcggcaggct
     2041 gattcccgcg acgtgggacg acgccatcaa gttcacggcg cagcggcttc aggagatcaa
     2101 ggacgcgcac ggcggcaact ccatcggcgt cgtcggcagc ccgcgcatca cgaacgaatc
     2161 gaacttcgcg ctgctcaagt tcgcgcgcga agtcttggag acggagaact acaccgcgac
     2221 cgaggcgttc tcgctcgcgc ccttcttcga gaacctcggc gccccgctcg ccacgcaccg
     2281 cgacatccgg cacgccaaaa ccatcgtcct catcggcggc gaacccgaag agaaccagcc
     2341 gctcaccggc agacagattc ggcaggcggt gcgcaacggc ggcgcgaagc tcgtcgtcgt
     2401 caacagcgtc ccgattcgtt tgaaggagca ggccgcgcag ttcatacaca tccttccggg
     2461 cacggaagac gcggcggccc tcgccttcac cttgcaggac agcgacggcc tcgccgcgcg
     2521 caagatgggc gtcgaggccg gcgaaatcga gagcgcgctc cacctcgtca acgacacgca
     2581 gggcgacgtg gttatcgtct tcggcggcga gctgagcgcc gaggcgcagg ccgtgctcgc
     2641 gcaggcgccg cacaacttcg gcgagcgcga gggccgccgc atcttcttcc acccgctgcc
     2701 gctctacaac aacagcgtcg gcgtccacga catcggcctg atgggcggcg cgctgaaccc
     2761 gctcgacatc ctcgacgcgg cgggcaccac ccttagcgcg atgtacgtcg cgggcagctt
     2821 cctccccgcg cacctcgaag ggcgcgagga cgcgctctcg aagctcgact tcctcgtcgt
     2881 gcaggagttg ttccagaccg cgacgaccga gcaggcggac gtggtgctgc ccgccgcctc
     2941 gttcgccgaa gttgacggca cgttcacgaa cagcggcggc ctcgtccagc gcgtgcgcca
     3001 gtcgatcccg cccgtccacc agtcgaagcc cgactgggtc atcacgtctt ccatcgcgtc
     3061 ggcgatgggc gcggacttcg gttatcaaat ggcttcgagc gccgtcttcc tcgacatagc
     3121 gcgcaacgtc gccgcttacg agggcttgcg ctacccgctc ctgaaggacg aaacgaaccc
     3181 gcggcaggcc gcgcacgcgc tcgccgggcg gcgcgacgtg tcgcggcaac tggaagactt
     3241 gcgcgcaatc gtcaacgaac tcgaagaggg cggcgagaag gtcacgggca cgcccccggt
     3301 cggccacgag cttttcaaga tcggcacgct gacggacaag gtgccgcagt tccacctgct
     3361 ggccgcgggc aaccccgaac ccgagagcgt cctgatttcc cccctctacc agatcacggt
     3421 tgaccctccc ttgcgccgcg gcgaagcggt cgcgggagac tgaggcgatt gcggatttcg
     3481 gattgcggat tttggattgt tcggactgtg aaagtcgaac gttcaaaacc cggcttcgag
     3541 tttggaaagc gcgctaatcc gaaatccgaa atccgcattc cgaaatcggc atatggactg
     3601 gcttaacgac actctcggcc cggcgaacgt gcggggcctc gggcagctca tcggcatcaa
     3661 cgtggcgttc gtcgcggtga tgctggtcgt ggcgtacacc gtactggcgg agcggcgcgt
     3721 gctggccttc atacaggggc ggctggggcc gaaccgcgtc gggccgggcg gcgtgctgca
     3781 accgttcgcc gacctcctga agttcatctt caaggaggag atcacgcccg ccaaggcgac
     3841 gcgcttcgtc tacttcctcg cgccgatgat cgccgtcatc gcggccttca tgccgatcat
     3901 cgtctacccg ttcggcccga ccgtctacct gcccttcctc ggcgacgtct cgctcgtcat
     3961 cgcgcggttg gacatcggcc tgctgttcgt gctgggcatc acgtcggtcg gcgtctacgg
     4021 catcatgctc gcgggctggg cctcgaacag caagtacagc ctgatgggcg gcctgcgcgc
     4081 gtcggcgcag atgatctctt acgagttgtc tctgggcctc tcgctcgtcg gcgtcgtgct
     4141 gctgtcgggg acgctcgacc tcttcaacgt cgtcgaacag cagtcgggat ggttcggcat
     4201 gaagtggaac atcctcttcc aaccggtcgg cttcatcgtg tatttgatct cggccatcgc
     4261 cgagacgaac cgcgtgccgt tcgacctgcc ggaggccgag acggagctgg tcgcgggctt
     4321 ccacacggag tattcggcgc tcaagttcgc gctcttcttc atggccgagt acgtcaatat
     4381 gttcacggtc tcgatgctgg cgacgacgct cttcctcggc ggctggaacg gccccttcgt
     4441 cgagcaggtg ccctacctcg ggccgctcta cttcttcggg aaggtcttct tcttcctctt
     4501 cctctacatc tggctgcgcg ggacgctccc gcgcttccgc ttcgaccagt tgatgaactt
     4561 cggctggaag ttcctgctcc ccgtcgccgt cctcaacatc atcgtgacgg ctacggtcgc
     4621 cttcttctgg tacggagcac aaacgccgtg aaccgtgaac cggaaaccgt gataagtgaa
     4681 aacggatttc ttcctgtcac ggttgacggt tgacggttca cggctcttga aagttatgtt
     4741 gacgattctc ctcttcatca tcttcggcgg gctggcggtg gggtgcgccg tggcggtggt
     4801 ggcacagcgc aacccgctct acagcgcgat ctcgctggtg ggcgtcttcg tcgcgctggc
     4861 gtgcctctac atgacgctcg ccgcgccgtt catcgccgcc gtgcaggtaa tcgtctacgc
     4921 gggcgcgatc atggtgctcg tcgtcttcgt catcatgctg ctcaacgtcg aggaggaggt
     4981 gcgccgcccc gtccgcctgc gctacctgat cccggtcgcg gtcgcgctcg cggcgatcct
     5041 cgtcgccgag acggccttca tgctgacgct gggcggcggc cccggcgcgc accgcttcga
     5101 cccggcatcc atcccgcaga cgggcgtcgg gctgacggcc tcggtcggca cggggctgtt
     5161 caccgaatac ctgctgccct tcgaggtcac gtcggtgctg atcctgatgg cgctcgtcgg
     5221 cgccatcacg ctcgcgcgca ggacgctggc ccccgccgtg accgcgcccg actacgcgct
     5281 cggccccacc gatttcagca tcggcgacga caatgtgccg cgcacgctcc ccgacgcagc
     5341 caccccggcg gaggccgtcc gccacggcga cacggagacc accgggcgcg agatggcgcg
     5401 cttgcagatc gagcaggaag aggccgagcg cgcgcgcgac cgcaaggcga agactgaaga
     5461 ggagggcgga caaagatgat ccctctgtcg tggtatctgg cgctttccgc cgtgctcttc
     5521 accatcgggg tcacgggcgt catcatcaag cgcaacgcga tccagatgtt catgtgcatc
     5581 gagctgatgc tcaacgccgt gaacctgacc ttcgtcgcct tcgcctccta cttcggggac
     5641 gtgaccggcc acctcttcgt cttcatcgtg atgacggtcg ccgccgccga ggccgcggtc
     5701 ggcctcggca tcatcatcgc catcttccgc aaccgcgagt cgctcgacgt ggacgaggcg
     5761 aacatcttga aactctaaga gccgtgaacc gtgaaccgtg aaccgtgaca aggagaacga
     5821 caggttttca ctcatcacgg ttgacggttc acggttcacg gcggttgcag cccttatgat
     5881 tcgctacatc atcttcgcgc cgctggtggg ggccgtcatt aactggctgg tggggcgccg
     5941 cgtgcggagc gagaggttcg tcggcctcgt ggcgtgcggg tcggtggcgg tgtcggcggc
     6001 cttcgcgttc tggctggcgt tcggccccga gggcgcgctc aggggcgagg agccgcgcgt
     6061 catcctcgac cacctgtgga cgtggattca ggtcgggggc ttccgcgccg ac
//
LOCUS       DBNGOH010001064         2308 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4011474, whole genome shotgun sequence.
ACCESSION   DBNGOH010001064 DBNGOH010000000
VERSION     DBNGOH010001064.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2308)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2308)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2308
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4011474"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(9..>2308)
                     /locus_tag="AC159P_20775"
     CDS             complement(9..>2308)
                     /locus_tag="AC159P_20775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HIR3697936.1"
                     /translation="DNFPTTVGAFDRTRSCCTPLGQGSFSIRDAFVARLSANGSALEY
                     STYIGGHGDEVPKSIVVDAAGFVTLTGLTFASPDTSAPRFPTTPGALMPNPSSGPTSA
                     DAFLARLKLDGNGSADLKYSTFIGGSDTDEGYAVALDPTNPNDVVVGGVTYSTVSTAR
                     FQTTAGTLRPSSDSVDGFVMKFRFPSTGGGQLVWSTLFGGFLYEEVSDLAVDGAGAVV
                     ISGHSNSFDLPTTAGAYDRTVAITSGLLFFDAYVARLSPDGARLLYGTYLGGGFNDEH
                     THLALTGPDTAVVSGWTKSHDLPVSPTAFDTVLNHDGAGGAAVPFDGFLARLTLLADG
                     DGDDTVSAPALLAPVVGAQVAVNDLVTFDWSDVADTSGVDGYHVQINRSPDFVCCNDW
                     IEVWSTDSEHVDSVRFDGPYYWRVQTADRSGNLSAWSEVRTFNAGTPPPPAAPVLVSP
                     PSGIRLPPNTNITFAWNAAAGAATYEIQIDDASSFSAPLVAFASGLVQTQSVHSFSRE
                     RRHWWRVRGRNSQGTAGSWSAVRNFEVKRGAPPPPPPPSPTPTPTPTPPPPTPTPTPP
                     PPTPTPTPVPTPTPPPPAALSSLTLNPTSVTGGNSAQGTVTLTAAAPLTGTIVALSSS
                     NTTAASVPAGVTVPAGATSASFTMFTSSVTASTAVTISASFGGTTRTATLTVNPVQTT
                     TDTVAVQLAEYDSGKRELRVEATSSNSGAVLRCFVTSTGALIGTLTNDGGGRYRGQFS
                     WPSNPQSVTVRSSLGGSATRTVAAK"
BASE COUNT          334 a          708 c          926 g          340 t
ORIGIN      
        1 gggccgcgct acttcgcggc caccgtccgc gtcgcggagc cgccgaggct gctgcgcacc
       61 gtgacgctct gcgggttcga gggccacgag aactggcccc tgtacctgcc gccgccgtcg
      121 ttcgtcagcg tcccgatgag cgcgccggtc gaggtcacga aacaacgcag cacggcgccc
      181 gagttgctgc tcgtcgcctc gacgcgcagc tcgcgcttgc ccgagtcgta ctcggccagt
      241 tggacggcga ccgtgtcggt cgtcgtctgt acagggttga ccgtgagcgt ggccgtgcgc
      301 gtcgtgccgc cgaaagaggc ggagatcgtc acggcggtcg aggccgtcac ggaggaggtg
      361 aacatcgtga agctcgcgga ggtggcgccc gccgggacgg tcacgcccgc gggcacgctg
      421 gccgcagtcg tattgctgct tgagagcgcg acgatggtgc cggtcaaagg cgcggcggcg
      481 gtcagcgtca ccgtgccctg cgcggagttg ccgccggtga cgctcgtcgg gttgagcgtg
      541 agcgacgaca gcgcggcggg cggcggcggc gtcggcgtcg gcacaggcgt cggagtcggt
      601 gtcggcggcg gcggcgtggg cgtcggcgtc ggtggcggcg gtgttggggt cggcgtcggt
      661 gtaggcgatg gcggcggtgg cggcggcggt gcgccgcgct tgacctcgaa gttgcgaacc
      721 gctgaccaag acccggcggt gccctgcgag ttgcgcccgc gcacgcgcca ccagtggcgc
      781 cgctctctgg agaacgaatg cacggattgc gtctgcacga gtcccgaagc gaaagcgacg
      841 agcggcgcgc tgaagctgct cgcatcgtcg atctgaatct cgtaggtggc ggcgcccgcc
      901 gcggcgttcc acgcgaaggt gatgttggtg ttcggcggca ggcggatgcc gctcgggggg
      961 ctgacaagca cgggggcggc gggcggcggc ggcgtgcccg cgttgaaggt gcgcacttca
     1021 gaccacgcgc tcaggttgcc ggagcggtcg gcggtctgca cgcgccagta gtaggggccg
     1081 tcgaagcgga cggagtccac gtgctccgag tccgtagacc agacctcgat ccagtcgttg
     1141 cagcagacga agtcggggct gcggttgatc tggacgtgat agccgtccac gcccgacgtg
     1201 tccgcaacgt ccgaccagtc gaaggtcacg aggtcgttga ctgcgacctg cgcgccgacg
     1261 acgggcgcga ggagggcggg cgcggagact gtgtcgtcgc cgtcgccgtc ggcgagcagc
     1321 gtgaggcgcg cgaggaagcc gtcgaagggc acggcggcgc cgcccgcgcc gtcgtggttg
     1381 agcacggtgt cgaaggcggt cgggctgacg ggcaggtcgt gcgatttcgt ccagccggag
     1441 acgaccgccg tgtccggccc cgtgagcgcg aggtgcgtgt gctcgtcgtt gaagccgccg
     1501 ccgaggtatg tgccgtagag caggcgcgcg ccgtcggggc tgagccgcgc gacgtaggcg
     1561 tcgaagaaga ggaggccgct cgtgatggcg accgtgcggt cgtaggcgcc cgcggtggtc
     1621 ggcaaatcga acgagttcga gtggcccgag atgacgacgg cgcccgcgcc gtcaaccgcg
     1681 aggtcggaga cctcttcgta gaggaagccg ccgaagagcg ttgaccagac gagctggccg
     1741 ccgcccgtcg aagggaagcg gaacttcatg acgaagccgt caacgctgtc ggagcttggg
     1801 cgcaaagtgc ccgcggtggt ctggaacctc gcggtgctga cggtcgagta ggtgacgccg
     1861 cccaccacga catcgttcgg gttcgtcggg tcgagcgcga ccgcgtagcc ttcgtccgtg
     1921 tcgctgccgc cgatgaaggt cgagtacttg aggtcggcag agccgttgcc atcgagcttg
     1981 aggcgcgcga ggaaggcgtc cgccgaggtc gggccgctcg aagggttggg catgagcgcg
     2041 ccgggcgtgg tcgggaagcg cggggcggag gtgtcgggcg aagcgaacgt gaggcccgtc
     2101 agcgtgacga agccagccgc atcgacgacg atgctcttcg gcacctcgtc gccgtggccg
     2161 ccgatgtagg tcgagtattc gagcgccgag ccgttcgccg agagccgcgc gacgaaggcg
     2221 tcgcggatgg agaagctgcc ctggccgagg ggcgtgcagc aactgcgcgt gcggtcgaag
     2281 gcgccgacgg tcgtcgggaa gttgtccg
//
LOCUS       DBNGOH010001065         2668 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_528678, whole genome shotgun sequence.
ACCESSION   DBNGOH010001065 DBNGOH010000000
VERSION     DBNGOH010001065.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2668)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2668)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2668
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_528678"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..882)
                     /locus_tag="AC159P_20780"
     CDS             complement(<1..882)
                     /locus_tag="AC159P_20780"
                     /inference="COORDINATES: protein motif:HMM:NF051540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HIR3697937.1"
                     /translation="MSAREGQTPRGAAPPHERALWWLIAGRVLVAGALLCVSALWARS
                     FWQGGTETSSFRRALLVALVVLVLSVVYALLLRFSRLPLRAQAAAQFFFDVLLITWLV
                     WATGDLHSPYSALYIVVIAVAGIFLGARGALLTAVGSAACYTSVMVSLVTGWLGNHGR
                     EAAPLGEAVEAIGINDVAFLVVGLLSSRLAERQTRSDVQLIEAAHALANLRALHERIV
                     ESIRSGVITTDLGRRIYTLNRAGEEMTGYAAEDLRGQDVSILFGDIRAEIEASLRAAA
                     AGHSAARYEAECLTAEGF"
     gene            1160..>2668
                     /locus_tag="AC159P_20785"
     CDS             1160..>2668
                     /locus_tag="AC159P_20785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HIR3697938.1"
                     /translation="MNNIETSFHNHRRPAATRARGYFLSALAVLSLVACGGVRPASVN
                     ASDVQPAPAATAAAPQQQNSYAPVVSRVAPAVVTVRSERRGRAAQQFPFMDDPRFRDF
                     FGDRLPRGEQQEPPQRRERGMGSGVVVRADGYILTNHHVVDGAEEITVELNDRRSFTA
                     KLVGSDPPSDLAVLKVEASALPVLALGDSDRVEVGDVVLAVGNPLGIGQTVTSGIVSA
                     KSRATGLSDGSFEDFIQTDAAINRGNSGGALVNTSGELIGINSQILSPSGGNIGIGFS
                     IPSNMAKNVMEQIIQTGKVRRGLLGVTLQAVTPDIAASRGLSTARGALVSGVTPGGPA
                     ERAGVRRGDVITALNGQPVNDSNALRNRVAATQPGAEVTFTVLRDNREQQLRATLGEL
                     PVQTSSANDTEEGGARPGATAEGSRLGVGVEPLTPEAATRLKLPRDTQGLLVGTVDPA
                     GPAAEAGVRRNDVIQEVNRQPVRTVEELRAALDRTGNRPALLLVTNEGGSGYV"
BASE COUNT          442 a          987 c          900 g          339 t
ORIGIN      
        1 gaagccctcg gcggtcaggc actcggcctc gtagcgcgcc gcggagtggc ccgccgcggc
       61 ggcccggagc gacgcctcga tctccgcgcg gatgtcgccg aagaggatcg acacgtcctg
      121 cccgcgcagg tcttccgccg cgtagccggt catctcctcg cccgcgcggt tcagcgtgta
      181 gatgcgccgc ccgaggtcgg tcgtgatgac gcccgagcgg atcgactcga cgatgcgctc
      241 gtgcagcgcg cgcaggttgg cgagcgcgtg cgccgcctcg atcagttgca cgtccgagcg
      301 cgtctgccgt tcggccagac gcgacgagag cagcccgacg acgaggaagg ccacgtcgtt
      361 gatgccgatg gcctcgaccg cttcgcccag gggagccgcc tcgcgcccgt ggttcccgag
      421 ccagcccgtc acgagcgaga ccatcacgga cgtgtagcag gccgcgctcc cgaccgccgt
      481 cagcagcgcc ccgcgcgcgc cgaggaagat gcccgccacc gcgatgacga cgatgtagag
      541 cgccgagtag ggcgagtgca aatccccggt cgcccacacg agccaggtga tgagcagcac
      601 gtcgaagaag aactgcgccg ccgcctgcgc ccgcagcggg agccgcgaga aacgcagcag
      661 cagcgcgtag acgaccgaga ggacgaggac gaccagcgcg accagcagcg cgcgccggaa
      721 cgaactcgtc tcggtgccgc cctgccagaa gctgcgcgcc cacagcgcgc tcacgcagag
      781 cagcgccccc gccaccaaca cgcgcccggc gatgagccac cacagcgcgc gctcgtgcgg
      841 cggcgccgcg cccctcggcg tctgaccctc tctcgcgctc atgggccgta agcctagcac
      901 aactggcgcg cggcgccctc aacttccgtc aactcctcaa ggggccgcgg ctccataaaa
      961 agtcaagaaa gctgaaaatg attttcgaga cgcgggcggc acactaagaa ggggactcgc
     1021 agcaaggccg cccgcgccgc ttagacagca tctaaacaac gtgccgccgg tctctattcg
     1081 tagcgcgcgg gccgaaggtt gcccgcaggt ctgattgacc ggcggggatt gattcatgac
     1141 aggtcgaggt ggaggagtaa tgaacaacat cgaaacatct tttcacaatc atcgacgccc
     1201 cgcggccacg cgcgcgcgcg gctactttct ttcggcgctg gccgtcctct cgctggtcgc
     1261 gtgcggcggc gtgcggcccg cgtccgtgaa cgcttccgat gtgcagcccg ctccggcggc
     1321 cacggcggcg gcaccgcagc agcagaactc gtacgcgccc gtggtgagca gggtcgcgcc
     1381 cgcggtcgtg accgtccgct cggagcggcg cggacgcgcg gcgcagcagt tcccgttcat
     1441 ggacgacccg cggttccgcg atttcttcgg cgaccgcctg ccgcgcggcg agcagcagga
     1501 gccgccgcag cggcgcgagc gcggcatggg gtcgggcgtc gtcgtccgcg ccgacggcta
     1561 catcctgacc aaccatcacg tggttgacgg ggccgaggag atcaccgtcg agctgaacga
     1621 ccggcgctcc ttcacggcga agctcgtcgg ctccgacccg ccgagcgatc tggccgtgct
     1681 gaaggtcgag gcgtccgctt tgcccgtgct cgcgctcggc gactccgacc gcgtcgaggt
     1741 gggcgacgtg gtgctcgccg tcggcaaccc tctgggcatc gggcagaccg tcacctccgg
     1801 catcgtcagc gcgaagagcc gcgcgacggg tctttccgac ggcagcttcg aggacttcat
     1861 ccaaacggac gccgccatca accgcggcaa ctcgggcggc gcgctcgtca acacgagcgg
     1921 cgagctgatc ggcatcaact cgcagattct cagcccttcg ggcggcaaca tcggcatcgg
     1981 cttctccatc ccctcgaaca tggcgaagaa cgtgatggaa cagatcatcc agacgggcaa
     2041 ggtgcggcgc ggcctgctcg gcgtcaccct ccaggccgtg acgcccgaca tcgccgcgag
     2101 ccgcggcctc tcgacggcgc gcggcgcgct cgtcagcggc gtcacgccgg gcggccccgc
     2161 cgaacgcgcg ggcgtccgtc gcggcgacgt gattacggcg ctcaacggcc aacccgtcaa
     2221 cgacagcaac gcgctgcgca accgcgtcgc ggcgacgcag cccggcgccg aagtcacctt
     2281 cacggtcttg cgcgacaatc gcgagcagca gctccgcgcc acgctcggcg agctgcccgt
     2341 ccaaacgtcg agcgcgaacg acaccgaaga gggcggcgcg cgtcccggcg cgaccgccga
     2401 agggtcgagg ctcggcgtcg gcgtcgaacc gctgacgccc gaggccgcca cgcggctcaa
     2461 gctcccgcgc gacacgcagg ggctgctcgt cggcaccgtc gatcccgcag gccccgcggc
     2521 tgaggcgggc gtccgccgca acgacgtgat acaggaggtc aaccgccaac ccgtccgcac
     2581 cgtcgaagag ctgcgcgccg cgctcgaccg caccggcaac cgccccgcgc tcctgctcgt
     2641 caccaacgag ggcggcagcg gctatgtc
//
LOCUS       DBNGOH010001066         2482 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4743999, whole genome shotgun sequence.
ACCESSION   DBNGOH010001066 DBNGOH010000000
VERSION     DBNGOH010001066.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2482)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2482)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2482
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4743999"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            49..426
                     /gene="rplT"
                     /locus_tag="AC159P_20790"
     CDS             49..426
                     /gene="rplT"
                     /locus_tag="AC159P_20790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020708095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HIR3697939.1"
                     /translation="MPRAKRGNKRTERRKKILDLAKGYRGTKSKLYRSAKESVERGLK
                     FAYTGRKLKKRDFRSLWIVRIGAAARVNGLNYSQFMHGLSLAGIELDRKVLADLAANS
                     PDAFTELAGQAKNAIANKQPAAV"
     gene            451..1545
                     /locus_tag="AC159P_20795"
     CDS             451..1545
                     /locus_tag="AC159P_20795"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HIR3697940.1"
                     /translation="MSSEVVDNREQVGAVREAFGREFERFARFRAGGAEALSLGDAEV
                     YLRELAELRTRHTGKKSALSGLMKLVGRVAAEERAAFGQSVQQLRAEIETALDEAAAA
                     LQQSVAEARVERERIDVTLPGRRPRRGHLHPITLVRQQIEDIFVSMGYTVEDGPEIET
                     EFYNFTALNIPELHPARSPQDTYYTTEGLALRSQTSTVQIRAMEQRGAPLRIIAPGRV
                     FRRDTPDATHNPMFFQVEGLLVDRGITMGDLKGTITEFLRRLVGPGTRTRFRPSYFPF
                     TEPSAEFDFTCFKCHGAGCRLCKGSGWIELGGSGMVHPNVLRVAGIDPEEFSGFAFGF
                     GIDRMCALRYGLDDIRLLFENDVRFLEQFF"
     gene            1594..>2482
                     /locus_tag="AC159P_20800"
     CDS             1594..>2482
                     /locus_tag="AC159P_20800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697941.1"
                     /translation="MRFFTDETFLLLGGCGMVGQQIAHEIARDLDPRLIILCGLTDEE
                     VERAVASLRRDFPQVSVTGAVGDVFVRSEWNPHGRGAGGRLDRRELLASEEHRAALYE
                     DVFLDREGAYGRSELVRLIRAYAPNVVIDSINTATAFSYQDVYTASDLARRTLDELKA
                     AQRGETAGENLAGLIGAAGEALDAVLISQSVPQLVRHVLFINDAMREAKTRLYLKVGT
                     TGTGGMGLNIPYTHSEDSPSATLMEKTAIAFAHTGLMYLMARTLGGPAVKEFKPAAMI
                     GYADLAYQPVRVSGRGKIGV"
BASE COUNT          425 a          830 c          857 g          370 t
ORIGIN      
        1 tccctcatcc ctccgccctc agcccttaaa agaagggagt tatcagtcat gcccagggca
       61 aaacgtggga acaagcgcac cgagaggcgc aagaagattc ttgatctggc gaagggctac
      121 cgcgggacga agtcgaagct gtaccgctcg gccaaagagt cggtcgagcg cgggctgaag
      181 ttcgcgtaca cggggcgcaa gctgaagaag cgcgacttcc gcagcctgtg gatcgtccgc
      241 atcggcgcgg cggcgcgcgt caacgggctg aactactcgc agttcatgca cggcctctct
      301 ctggccggca tcgaactcga ccgcaaggtg ctcgcagacc tcgcggccaa ctcgcccgac
      361 gccttcaccg aactggcggg gcaggcgaag aacgcgattg cgaacaagca gccggcggct
      421 gtttagtaaa caaagatatg aggactccgc atgagttccg aagtggttga taaccgcgaa
      481 caagtcggcg cggtgcgtga agcgttcggg cgagagttcg agcgcttcgc gcgctttcgc
      541 gcgggcggag cggaagcgct ttcgctcggc gacgccgaag tctacttgcg cgagctggcc
      601 gagctgcgga cgcggcacac ggggaagaag agcgcgctct cggggctgat gaagctcgtc
      661 gggcgcgtcg cggcggagga gcgcgcggcg ttcggccaga gcgtgcagca gctccgcgcg
      721 gagatcgaga cggcgcttga cgaagccgcc gccgcgttgc agcagtcggt ggccgaggcg
      781 cgcgtcgagc gcgagcgcat cgacgtgacg ctgcccggtc gtcgcccccg tcgcgggcac
      841 ctgcatccga tcactctggt gcggcagcag atcgaggaca tcttcgtctc gatgggctac
      901 acggtggagg acgggccgga gatcgagacg gagttctaca acttcaccgc actcaacatc
      961 ccggagctgc accccgcgcg cagcccgcag gacacctact acacgaccga aggactggcg
     1021 ctgcgttcgc agacctcgac cgtgcagatt cgcgcgatgg agcagcgcgg cgcccccttg
     1081 cgcatcatcg cgccgggccg cgtcttccgg cgcgacacgc cggacgcgac gcacaacccg
     1141 atgttcttcc aggtcgaagg gctgctggtt gatcgcggca tcacgatggg cgatttgaag
     1201 gggacgatca cggagttcct gcgccgcctc gtcggccccg gcacccggac gcgctttcgg
     1261 ccctcctact tcccgttcac ggagccgagc gcggagttcg acttcacctg cttcaagtgc
     1321 cacggcgcgg gctgccgcct gtgcaaaggc tcgggctgga tcgagctggg cggctcgggc
     1381 atggtgcacc cgaacgtgct gcgcgtggcc ggcatcgacc ccgaggagtt cagcggcttc
     1441 gccttcggct tcggcatcga ccgcatgtgc gcgctccgct acgggctgga cgacatccgc
     1501 ctcctcttcg agaacgacgt gcggttcctc gaacagttct tctaagcgga cggcggcgct
     1561 gcgacacctc atccgaccgg gagggagagc cacatgcgct tcttcacaga cgagacgttt
     1621 ctgctgctcg gcgggtgcgg catggtcggg cagcagatcg cgcacgagat cgcgcgcgat
     1681 ctcgacccgc gcctcatcat cctctgcggc ctgaccgacg aggaagtgga acgcgcggtc
     1741 gccagtctcc gccgcgactt cccgcaggtc tccgtgacgg gcgcggtggg cgacgtgttc
     1801 gtccgcagcg agtggaaccc gcacgggcgc ggggccggcg gaaggctcga ccgccgcgag
     1861 ctgctggcga gcgaggagca ccgcgcggcg ctctacgaag acgtgttcct cgaccgcgag
     1921 ggggcgtacg ggcgctcgga gctggtacgg ctcatccgcg cctacgcgcc gaacgtcgtc
     1981 atcgacagca tcaacaccgc gaccgccttc agctaccagg acgtgtacac cgcgtccgac
     2041 ctcgcgcggc gcacgctcga cgagctgaag gcggcgcagc gcggcgagac cgccggcgaa
     2101 aatctcgcgg ggctgatcgg cgcggcgggc gaggcgctcg acgcggtgct gatctcgcag
     2161 tccgtgccgc agctcgtccg ccacgtcctc ttcatcaacg acgcgatgcg cgaggcgaag
     2221 acgcgcctct acctgaaggt cgggacgacg ggcacgggcg gcatgggcct gaacatcccc
     2281 tacacgcatt ccgaggactc gccgtcggcg acgctgatgg agaagacggc catcgcgttc
     2341 gcgcacacgg ggctgatgta cctgatggcg cgcacgctcg gcggccccgc ggtgaaggag
     2401 ttcaagcccg ccgcgatgat cggctacgcc gacctcgcct accagcccgt ccgcgtcagc
     2461 ggcaggggca agatcggcgt gc
//
LOCUS       DBNGOH010001067         8195 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1813008, whole genome shotgun sequence.
ACCESSION   DBNGOH010001067 DBNGOH010000000
VERSION     DBNGOH010001067.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8195)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8195)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8195
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1813008"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(257..2137)
                     /locus_tag="AC159P_20805"
     CDS             complement(257..2137)
                     /locus_tag="AC159P_20805"
                     /inference="COORDINATES: protein motif:HMM:NF013276.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HIR3697942.1"
                     /translation="MKRTSLLERAAARLALVFLLAVTAGEVAASAADTTVAADGTGQF
                     KSVQDAINAAPPVRPGRRWVIHIKPGVYKELIYLQREKRFISLVGEDASKTVLTYDLN
                     ANTQGADGKPLGTFRTASTTVDADDFAAENITFENAAGPVGQALAIRLDGDRAAFRNC
                     RFLGWQDTILANRGRHYFENCYVAGHVDFIFGAATAFFERCHLHALRDGYLTAASTPE
                     DQPHGFVFSNCRVTAETPSVKTYLGRPWRPFASVAFINTEMAAGVRPEGWHNWGHAVR
                     EKTVRYAEYRSTGAGAGAQARVPWARQLTEAEAANLTAEKVLAGHDNWNPRAKETRPP
                     VTNVTSVTDAPRADAANVAAAAAPCLSRGGLKADIEYSRAGGESLKLDACVPDGAGPF
                     PAAILVHGGGWSGGDKAKGIDPLFAPLARAKVAWFSVNYRHAPKHRYPASVEDVEAAV
                     RWVKAHAAEFRIDPRRLALVGESAGGHLVALAVVRAKEETRVAAVVPFYAPVDLEADT
                     ERRGGLSASLKALFGREKVDEDMLRVLRESSPINHVREGLPPFLLVHGTGDMSVPYNQ
                     STRMQAKLREAKVPAELITIDGGPHGMARWEDLDPTYKEKVATWLAQRLGAAPPAQSV
                     VK"
     gene            2293..4158
                     /locus_tag="AC159P_20810"
     CDS             2293..4158
                     /locus_tag="AC159P_20810"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF012772.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HIR3697943.1"
                     /translation="MAGPAGTRRQPQDASDPMRARILVADDEPLVREVLCDLLGRDYE
                     CREVSCAEDALALLGEEEFALVLSDINMGGLSGLDMLPRVAEIAPATLVIMISGGQEI
                     NDAVRARRAGAFDYITKPFDIEHVEAAVRRALEHHGLQQSKRRYEGYLEALLKQRTAE
                     LTHVSYHDALTGLPNRVLFEDRLAQALAAPDRGGVQSSALMLLDLDRFKKINESLGYQ
                     AGDRVLREVATRLLDSCAGESVTVARFGSDDFALLVPRVEGARDAIRIAYLIREAFQP
                     SFDINGHELYITASIGVSLSPCDGADSETLLRNAGAALRRAREHGGGLYEFYTADMNA
                     EALKRFAMESDLRRALERAEFVVHYQPQYRIAAEGAGARALRISGAEALVRWRHPEFG
                     LIPPAEFIPLAEGTGLIVRLGEWVLRTACAQNRAWQAAGFSPFRVAVNLSARQFHDRR
                     LEESVARALADAALDPRWLELELTESLLAKDAGRAVETLTALRELGLKVSVDDFGTGY
                     SSLSYLIDLPIDTLKIDRAFVRDAVVNTRHATLLRGIIALAHDLKLGVKAEGVETSEQ
                     RDMLCQLGCDEMQGFLFSRPVPAAEFEAMLRREQQAAHTGSAPPGHQLPRGYRRA"
     gene            4313..5908
                     /locus_tag="AC159P_20815"
     CDS             4313..5908
                     /locus_tag="AC159P_20815"
                     /inference="COORDINATES: protein motif:HMM:NF024850.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR3697944.1"
                     /translation="MVTLLVAVSLLAGDEAADARHATSQEATASQEARPLTPQERRGK
                     AIYLRGESASGREIAAAIGELDVPASTVTCAGCHGLRGEGKTEGGVTAGALTWSHLVK
                     PYGHTHPSGRKHGPFDEAAFARAVSKGIDPDGNDLLVAMPRYRMAAEDLADLVAYLKR
                     IESERDPGLDAGTVRVGVPLPSKGALAETGAAMKEVLAAYFAGVNERGGIYNRKVELS
                     VADAGDAAGVEAARASVRRGEVFAFVGGVTAGVDREVAALARDEEIPFVGAVTLLPQS
                     EAPPNRYVFYFLPGVREQARALVNFAAARPELKPAHAVVLHADNELATAAAVAAEEQA
                     KKHGWGAVTRQPYARGGFDAAQLAKSLKQQNAGAVFLFGGEDAALLREAAAIDWTPRV
                     FLLGVLAAKELPATLPVAFKDKVFLSFPTVPSDLTPAGLSELAALRDRYKLTSRHTAS
                     QLSALAAAKVFVEALKRAGHDLTREKLITALEGFYDYETGLTPRLTFGPNRRVGAAGA
                     YLVTVNPDTKDFTPAAAGWVNAN"
     gene            6100..>8195
                     /locus_tag="AC159P_20820"
     CDS             6100..>8195
                     /locus_tag="AC159P_20820"
                     /inference="COORDINATES: protein motif:HMM:NF024603.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing domain"
                     /protein_id="HIR3697945.1"
                     /translation="MTRLKNAAVALLTIQLIVGALSPLVPQTHAGDSGAAVKETEEMS
                     QDSAAAGGDAVPKGLRFRLSEGAEESEQRPAAAASPAPAEKLSETEAARVLQRLPPLK
                     ADEGDEQDFDLREKSQPAPRTGRTVLASFPANEAREAPDAAAAGPLQVLRQSPAGDVP
                     LASQVTASFSQPMVAVTSQTEASETVPVRLSPQPEGRWRWLGTKTLLFDAAGERLPMA
                     TEFTATVPAGTRSATGGVLGAPVVWKFATPPPGLKTKYPEGGPVRRDAIIFMEFDQKV
                     DPAGVLRTLKVRGGTTFLKVRPATDDEIAADEVVKGLSAAAVKGRWLALRAVDARGSV
                     GDALPGNASIIVTVGPGTPSAEGPRVTAQAQAFAFNTYGPLRVNGQTCGWDARCTPFD
                     EWQVNFTNPLDAQAFSKSQVRISPELPDAKVELYGNTIQVRGAKRGRTKYTVTIDGAL
                     RDEFGQTLGAPAVVTFNVGAADPTLFSTGDALVVLDPSGEPRFPVYTVNHRTLKVTLY
                     AVAPEDWWRYMSHLRHVNGHNDDEAKRQTTPPGRQVYSKTIEVKQQPDELTETRLDLA
                     PALKEGHGQVIVVVESTLPVKKHEQREALRSWVQATGIGLDAFVDGEGLVGWTTSLKD
                     GQPLAGVEVRVLPSGVRGASGADGVARLVFGETGGVTSLLVARRGDDVAILPEHDSWW
                     DRGTRWVKHIPLDSLR"
BASE COUNT         1259 a         2826 c         2909 g         1201 t
ORIGIN      
        1 atcgagggca ccgtttccca cccgacgccg cgcgcaacgc tgcgcgccgt gcgcgccgtg
       61 cgccgctgcg acgcggcggc gtgaagcgga agagagggcg aaagcagcgc gaagaggatg
      121 gcgaagaagg tcgtgtgatg aatgagtcgg tgcatggtca ttgctctcat gtgctttccc
      181 tccgattatg gacaagtccg gtgcgcacct ttcgacgcga atgatccgtc acaacgtcga
      241 gtttgggaac ccgtcatcac ttcacgaccg actgcgcggg cggcgccgcg ccgagtcttt
      301 gcgcgagcca tgtggcgacc ttctctttgt aggtcgggtc gaggtcttcc cagcgggcca
      361 tgccgtgcgg gccgccgtcg atggtgatga gttcggcggg cactttggcc tcgcggagct
      421 tggcctgcat ccgggtcgat tggttgtagg ggacgctcat gtcgcccgtg ccgtggacga
      481 ggaggaaggg cggcagccct tcgcgcacgt ggttgatggg cgacgactcg cgcaggacgc
      541 gcaacatgtc ttcgtccacc ttctcgcgcc cgaagagtgc tttgagcgag gcgctgaggc
      601 cgccgcgccg ctctgtgtcg gcttcgagat cgacgggggc gtagaagggc acgaccgcgg
      661 cgacgcgcgt ctcttccttc gcgcgcacga cggccagcgc gacgaggtgg ccgcccgccg
      721 actcgccgac gagcgcgagg cgtcgcgggt cgatcctgaa ctcggcggcg tgcgccttga
      781 cccagcggac ggcggcttca acgtcttcga cggaggcggg gtagcggtgc ttcggcgcgt
      841 ggcggtagtt gacggagaac cacgccacct tcgcgcgcgc gagcggcgcg aagagcggat
      901 cgatgccctt cgccttgtcg ccgcccgacc agccgccgcc gtgcacgagg atggcggcgg
      961 ggaacggccc cgcgccgtcg ggcacgcagg cgtcgagctt caggctctcg ccgcccgcgc
     1021 ggctgtactc gatgtccgcc ttcaggccgc cgcgcgagag gcagggcgcc gcggcggcgg
     1081 cgacgttcgc cgcgtcggcg cgcggagcgt cggtgacgct ggtgacattc gtaaccgggg
     1141 gccgcgtctc tttcgcgcgc gggttccagt tgtcgtgacc ggcgaggact ttttcggcgg
     1201 tgaggttcgc ggcctcggcc tcggtcaact gtctggccca cggcacgcgc gcctgcgcgc
     1261 cggcgcccgc gccggtgctc ctgtactcgg cgtagcgcac ggtcttctcg cgcacggcgt
     1321 ggccccagtt gtgccagcct tcggggcgga cgcccgcggc catctcggtg ttgatgaagg
     1381 cgacgctggc gaacggtcgc cacggtcgcc cgaggtaggt tttgaccgag ggcgtctcgg
     1441 cggtgacgcg gcagttcgag aagacgaagc cgtgcggctg gtcttcgggc gtcgaggcgg
     1501 cggtgaggta gccgtcgcgc aacgcgtgca ggtggcagcg ctcgaagaag gccgtggcgg
     1561 cgccgaagat gaaatcgacg tggcccgcga cgtagcagtt ctcgaaataa tgacgaccgc
     1621 ggttggcaag tatcgtgtcc tgccatccga ggaagcggca gttgcggaag gcggcgcggt
     1681 cgccgtcgag ccggatggcg agcgcctgcc cgacggggcc ggcggcgttc tcgaaggtga
     1741 tgttctccgc cgcgaagtcg tcggcgtcaa cggtcgtgga ggcggtgcgg aaggtgccga
     1801 ggggcttgcc gtcggcgccc tgtgtgttcg cgttgaggtc gtaggtgagg acggtcttcg
     1861 acgcgtcttc gccgacgaga ctgatgaagc gcttctcgcg ctgcaagtag atcagctctt
     1921 tgtagacgcc gggcttgatg tggatgaccc agcggcggcc cggcctgacg gggggcgcgg
     1981 cgttgatcgc gtcctgcacg gacttgaact ggcccgtgcc gtccgcggcg acggtggtgt
     2041 ccgccgccga ggccgcgacc tcgcccgccg tgaccgccaa gagaaacaca agcgcgagcc
     2101 tcgccgccgc gcgttcgagt aacgacgttc gcttcatcgt ccgcctctcc gctgttcttc
     2161 gaatgggcgc atcttatcaa gtccgacggc cacgtgctat tcgccgccgg gccgaaagtt
     2221 gtaaatctcc cccgcaatct gctctaatca agacggctct cacgccacac gccacagcgg
     2281 aggagacttc cgatggccgg ccccgccggg acacgcaggc agccgcaaga cgcgagcgac
     2341 ccgatgcgcg cacgcatcct cgtcgcggac gacgagccgc tcgtccgcga ggtcttgtgc
     2401 gacctgctgg gccgggacta cgagtgccgc gaggtctcgt gcgcggagga cgcgctggcg
     2461 ctcttgggcg aggaagagtt cgcgctcgtg ctgagcgaca tcaacatggg cggcctgagc
     2521 ggactcgaca tgctgccgcg cgtcgccgag atcgcgcccg cgacgctcgt catcatgatc
     2581 agcggcgggc aggagatcaa cgacgcggtg cgcgcgcggc gcgccggggc cttcgactac
     2641 atcaccaagc ccttcgacat cgagcatgtc gaggccgccg tgcgccgcgc gctcgaacac
     2701 cacgggctgc aacagtcgaa gcgccgctac gagggctacc tggaagcgct gctcaaacag
     2761 cgcacagcgg agctgacgca cgtctcctac cacgacgcgc tgacgggatt gccgaaccgc
     2821 gtgctcttcg aagaccgcct cgcgcaggcg ctcgccgcac ccgaccgcgg cggcgtgcag
     2881 tcgtcggcgc tgatgctgct cgacctcgac cgcttcaaga agatcaacga gtctctgggc
     2941 taccaggcgg gcgaccgcgt gctgcgcgag gtggccacgc ggctgctcga ttcgtgcgcg
     3001 ggcgagtccg tcacggtcgc gcgcttcggg agcgacgact tcgcgctgct cgtcccgcgc
     3061 gtcgagggcg cacgcgacgc catccgcatc gcctacctca tccgcgaagc cttccagccc
     3121 tcgttcgaca tcaacggcca cgagctgtac atcacggcga gcatcggcgt cagcctctcg
     3181 ccctgcgacg gcgcggactc cgagacgctg ctccgcaacg cgggggccgc gctccggcgc
     3241 gcgcgcgagc acggcggcgg cctctacgaa ttctacaccg ccgacatgaa cgccgaggcg
     3301 ctcaagcgct tcgccatgga gagcgacctc cggcgcgcgc tcgaacgcgc cgagttcgtc
     3361 gtccactacc agccgcaata ccgcatcgcc gccgagggcg cgggcgcgcg cgcgctccgc
     3421 atcagcggcg ccgaggcgct cgtgcgctgg cggcacccgg agttcggcct gatcccgccc
     3481 gccgagttca tcccgctcgc cgaagggacg ggcctcatcg tgcggctcgg cgagtgggtc
     3541 ttgcgcacgg cctgcgcgca gaaccgtgcg tggcaagccg cgggcttcag ccccttccgc
     3601 gtcgccgtca acctctcggc gcggcagttc cacgaccggc gcctcgaaga gagcgtcgcg
     3661 cgcgcgctcg cggacgccgc cctcgacccg cgctggcttg agctggagct gaccgagagc
     3721 ctgctcgcca aggacgccgg gcgcgcggtc gagacgctga cggcgctgcg cgagctgggc
     3781 ctcaaagtct ccgtcgatga tttcggcacc ggctattcga gcctgagcta tctcatcgac
     3841 ctgccgatcg acacgctcaa gatcgaccgc gccttcgtcc gcgacgccgt cgtcaacacg
     3901 cgccacgcga cgctcctgcg cggcatcatc gcgctcgcgc acgacctcaa gctcggcgtc
     3961 aaggccgagg gcgtcgagac ctcggagcag cgcgacatgc tctgccagct cggctgcgac
     4021 gagatgcagg gcttcctctt cagccgcccc gtccccgccg cggagttcga ggccatgctc
     4081 cgccgcgagc aacaggcggc gcacaccggc agcgccccgc ccggccacca actaccccgc
     4141 ggctatcgac gcgcgtgaga cgaaaagcag taggcagtag gcagtcaaga aagacccgag
     4201 ttgaaattcg aaatccggaa tctgggattc gaagttgtct tgactgccta ctgcctacta
     4261 actgtaggca atgacgagta tgggaagagt gagaatcctg atcgcttctg taatggtaac
     4321 gctcctcgtc gcggtctcgc tcctcgcggg cgacgaggcc gcggacgcac gccacgcgac
     4381 ctctcaagaa gcgacggcct cgcaggaggc gcgccctctt acgccgcagg agcggcgcgg
     4441 caaggccatc tacctgcgcg gcgagtcggc ttcgggccgt gagatcgccg ccgccatcgg
     4501 cgagctggac gtgcccgcct cgaccgtcac gtgcgcgggc tgccacggcc tgcgcggcga
     4561 gggcaagacc gagggcggcg tcacggcggg cgccctcacc tggtcgcacc tcgtcaagcc
     4621 ctacggccat acgcacccct cggggcgcaa gcacggcccc ttcgacgagg ccgccttcgc
     4681 ccgcgccgtc tcgaagggca tcgatcccga cggcaacgac ctgctcgtcg cgatgccgcg
     4741 ctacaggatg gccgcggagg atttggccga cctcgtcgcc tacctcaagc gcatcgagtc
     4801 ggagcgcgac cccggcctcg acgcgggcac cgtcagggtc ggcgtgccgc tgccgtcgaa
     4861 gggcgcgctc gcagagaccg gcgccgcgat gaaggaagtg ctcgcggcct acttcgccgg
     4921 cgtcaacgag cgcggcggca tctacaaccg gaaggttgaa ctcagcgtgg cggacgcggg
     4981 cgacgcggcg ggcgtcgagg cggcgcgcgc ctccgtgcga cgcggagagg tcttcgcctt
     5041 cgtcggcggg gtgacggcgg gcgtggacag ggaagtggcc gcgctcgcgc gcgacgaaga
     5101 gattcccttc gtcggcgcgg tgacgctgct accgcagtcg gaggcgccgc ctaaccgcta
     5161 cgtcttctac tttctgcccg gcgtgcgcga gcaggcgcgc gccctcgtca acttcgccgc
     5221 cgcgcggccc gagcttaaac cagcgcacgc cgtcgtcctc cacgccgaca acgagctggc
     5281 gaccgccgcc gccgtcgccg ccgaagagca ggcgaaaaag cacgggtggg gtgccgtcac
     5341 gcgccagccc tatgcacgcg gcggcttcga cgcggcgcaa ctcgccaaat ctctcaagca
     5401 acagaacgcg ggtgcggtct tcctcttcgg cggcgaagat gccgcgctcc tcagagaggc
     5461 cgcggcgata gactggacgc cgcgcgtctt cctcctcggc gtcctcgccg cgaaggaact
     5521 ccccgcgacg ctgcccgtcg ccttcaagga caaggtcttc ctctccttcc cgaccgtccc
     5581 ctccgacctg acgcccgcgg gcctctcgga actcgccgcg ctgcgcgaca ggtacaaact
     5641 cacttcgcgc cacacggcct cgcaactctc cgccctcgcc gccgcgaagg tcttcgtcga
     5701 agccctcaag cgcgcgggcc atgacctcac ccgcgagaaa ctcatcacgg ccctcgaagg
     5761 tttctacgac tacgagaccg gcctcacccc gcgactcacc ttcggcccca accgccgcgt
     5821 cggcgccgcc ggtgcctacc tcgtcaccgt caaccccgac accaaagact tcacccccgc
     5881 cgccgccggg tgggtcaacg ccaactgaat cggagacaac agcggcccga ctcgcgtcca
     5941 tcaacacgtg cgacttcagc cgcgcgaccg tgcccctcac gccgcccgcg cgccgcaaga
     6001 cattcacgcc gcgacgttct ataacagcgg aggcggggcc gccgcgcgcg gcttcgcctg
     6061 ctgacttcgc cagattcaga cgtccgccga ggagcttcga tgacccgact gaaaaacgcc
     6121 gccgtcgctt tgctgacgat tcaactgatc gtgggcgcgc tctcgcccct cgtcccgcag
     6181 acgcacgccg gagactcggg tgcagccgtg aaggagaccg aagagatgag tcaggactcc
     6241 gccgccgcgg gcggcgacgc cgtgccgaag gggttgcgct tccgcctgag cgagggcgcc
     6301 gaagagtcgg agcagcggcc agcggcggcg gcgtcgcccg cgcccgccga gaagctgtcg
     6361 gagacagagg cggcgagggt gttgcagcgt ctaccgccgc tcaaggcaga cgaaggggac
     6421 gagcaggact tcgacttgcg cgagaagtcg cagccggcgc cgcggacggg gcggacggtg
     6481 ttggcgtcgt tccctgcgaa cgaggcgaga gaggcgccgg acgccgcggc ggcggggccg
     6541 cttcaggtgt tgcggcagtc gcccgcgggc gacgtgccgc tggcctcgca ggtcacagcg
     6601 tcgttctcgc agccgatggt ggcggtgact tcgcagacgg aggcgtcgga gacggtgccc
     6661 gttcgtctgt cgccgcagcc cgaagggcgc tggcgctggc tggggacgaa gacgctgctg
     6721 ttcgacgcgg cgggcgagcg cctgccgatg gcgaccgagt tcacggcgac ggtgcccgcg
     6781 ggcacgaggt ctgcgacggg cggcgtgctc ggcgcgccgg tcgtgtggaa gttcgcgacg
     6841 ccgccgccgg ggttgaagac gaagtacccg gagggcggcc ccgtccgccg cgacgcgatc
     6901 atcttcatgg agttcgacca gaaggttgac cccgcggggg tcttgcggac gttgaaggtg
     6961 cgcggcggga cgacgttcct gaaggtcagg ccggcgaccg acgacgagat agcggcggac
     7021 gaggtcgtca agggactgtc ggcggcggcg gtgaagggcc gctggctggc gctgcgcgcg
     7081 gtggacgcgc gggggagcgt cggcgatgcg ctgccgggca acgcgagcat catcgtcacg
     7141 gtcgggccgg ggacgccttc cgcagagggg ccgcgcgtga cggcgcaggc gcaggcgttc
     7201 gcgttcaaca cgtacgggcc gctgcgcgtg aacgggcaga cgtgcggctg ggacgcgcgg
     7261 tgcacgccgt tcgacgagtg gcaggtgaac ttcacgaacc cgctcgacgc gcaggcgttc
     7321 tcgaagtcgc aggtgcgtat ctcgcccgag ctgcccgacg cgaaggtcga actctacggc
     7381 aacacgatac aggtgcgggg cgcgaagcgc gggcgcacga agtacacggt gacgatcgac
     7441 ggcgcgctgc gcgacgagtt cgggcagacg ctcggcgcgc ccgcggtcgt gacgttcaac
     7501 gtcggcgccg ccgacccgac gctcttctcg acgggagacg cgctggtcgt gctcgaccct
     7561 tcgggcgagc cgcgcttccc ggtctacacc gtcaaccaca ggacgctcaa ggtgacgctc
     7621 tacgcggtcg cgcccgagga ctggtggcgt tacatgtcgc acctgcgcca cgtcaacggc
     7681 cacaatgacg acgaggcgaa gcggcagacc acgccgccgg gcaggcaggt ctactcgaag
     7741 accatcgagg tgaagcagca gccggacgag ctgacggaga cgcgcctcga tcttgcgccc
     7801 gcgctcaagg aagggcacgg gcaggttatc gtggtcgtcg aatcgacgct gcccgtgaag
     7861 aagcacgagc agcgcgaggc gcttcgctcg tgggtgcagg cgacgggcat cggcctcgac
     7921 gccttcgtcg atggcgaagg gctggtcggg tggacgacct ccctgaagga cgggcagccg
     7981 ctcgcgggcg tcgaagttcg cgtgttgcct tcgggcgtgc gcggcgcgag cggcgccgac
     8041 ggcgtggcgc gtctggtgtt cggcgagacg ggcggcgtta cgtcgctgct cgtcgcgcgg
     8101 cgcggcgacg acgtggcgat cctgcccgag cacgactcgt ggtgggacag ggggacgcgc
     8161 tgggtcaagc acatcccgct ggacagcctg cgctg
//
LOCUS       DBNGOH010001068         2034 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1445204, whole genome shotgun sequence.
ACCESSION   DBNGOH010001068 DBNGOH010000000
VERSION     DBNGOH010001068.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2034)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1445204"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..830
                     /locus_tag="AC159P_20825"
     CDS             <1..830
                     /locus_tag="AC159P_20825"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HIR3697946.1"
                     /translation="GATSSLLHAVGVKHGGRRVEVVLRRFVDAGWLKTEPDLARHEAA
                     SLLKAAGASVPVPELIAFDESGEECGAPATLMTRLGGRVELMPPDVGAWLRGLAGAVV
                     PFHEIEAGDHPWSYFPYVNHLSAFEPPPWSRFPELWKKVFEIVAGPRPPARECFIHRD
                     YHPNNVLWQDGRVSGVVDWVNACRGAAGFDVAWCRLNLAQLCGVAEADEFLRAYSAHA
                     GASPEHQTYWDMMAVVEVLPGPPGVYEGWLAFGVGHLSEPLMRERLDEYLVSLVSRL"
     gene            989..2014
                     /locus_tag="AC159P_20830"
     CDS             989..2014
                     /locus_tag="AC159P_20830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697947.1"
                     /translation="MKKNIHVALFALAAASAIFAAPLSAGAQRRTNTPPPAAAAQPWA
                     APLPASDAVLVADMRRLIAEAVPRVFDNDAKRVADVNADIEQFRQRTGIDARQFDTVA
                     VGARLVHPREGVTKVDHMVAVARGKFDAAAVVAAGRAAAAGRLAEQKHAGKTVYVFSL
                     NEQLRLFGLVKMRVSDLGLAVLDDATLAVGEPAAVRAAIDAQAGRGGVDKALLNTARQ
                     PGALVGFAGNVPEGAFKDLDTGLPEVDKSLASIRRFHGSVGMSGTGFQLQTILVTQSA
                     QDASRLRGTIDAVRSVAPALISMAGERGQLAKSAIQNIKVTAQGTDVQLRLDLAQNEL
                     AAIMRVL"
BASE COUNT          311 a          663 c          756 g          304 t
ORIGIN      
        1 cgggcgcgac ctcttccctg ctacacgccg tcggggttaa gcacggaggt cggcgcgtcg
       61 aagtggtgct gcgccggttc gtggacgcgg ggtggctgaa gacggagcct gacctggcgc
      121 ggcacgaggc ggcgagtttg ttgaaggcgg cgggggcgag cgtgccggtg ccggagctga
      181 tcgcgttcga cgagagcggc gaggagtgcg gcgccccggc cactttgatg acgcggctcg
      241 gggggcgtgt cgagttgatg ccgccggatg tcggcgcgtg gctgcgcggt ctggcggggg
      301 cggtcgttcc cttccacgaa atcgaagcgg gcgaccaccc gtggagctac ttcccttacg
      361 tcaatcacct ctccgcgttc gagccgccgc cgtggtctcg gttcccggaa ctatggaaga
      421 aggtcttcga gatcgtggcg gggccgaggc cgcccgcgcg cgagtgcttc atccaccgcg
      481 actaccaccc gaacaacgtc ctgtggcagg atgggcgggt cagcggcgtg gtcgattggg
      541 tcaacgcgtg ccgcggcgcg gcgggcttcg acgtggcgtg gtgccgcctg aacctcgccc
      601 agctttgcgg cgtggcggag gccgacgagt ttctccgcgc gtacagcgcg cacgcgggcg
      661 caagccccga gcatcagacg tactgggaca tgatggccgt cgtcgaagtg ctgcccggcc
      721 cgcccggcgt ctacgagggc tggctcgcgt tcggcgtcgg ccacctgagc gaaccgttga
      781 tgcgcgagcg actggacgaa tatttggtga gtctggtgtc gcgcttgtga gtcgagccgc
      841 ccttgagagt taagccgcgc cgcctgtttc aggccaaccc ccacacgcgc gcggcgcatc
      901 cgaacccccg aacgatactc ccccgcattt cgttaacggc gccttcgtgc gcgccgtcaa
      961 tcaagggaaa ggtgtagacc gaagggttat gaaaaagaac atccacgtcg cgctcttcgc
     1021 gctcgccgca gcgtcggcga ttttcgccgc gccgctttcc gcgggggcgc agcgcaggac
     1081 caacacgccg ccgccagcgg cagcggcgca accgtgggcg gcgccgctgc cggcgtcgga
     1141 cgcggtgctg gtcgcggaca tgcggcggct gatcgccgag gccgtgccgc gcgtcttcga
     1201 caacgacgcg aagcgcgtgg cggacgtgaa cgcggacatc gagcagttca ggcagcgcac
     1261 aggcatcgac gcgcggcagt tcgacacggt ggccgtcggc gcgcgccttg tgcacccgcg
     1321 cgagggcgtg acgaaggtcg atcacatggt cgccgtggcg cgcgggaagt tcgacgcggc
     1381 ggcggtcgtc gccgcgggcc gcgccgcggc ggcgggcagg ctcgccgagc agaagcacgc
     1441 gggcaagacc gtctatgtct tcagcctcaa cgaacagttg aggctcttcg ggctggtgaa
     1501 gatgagggtc agcgacctcg ggttggcggt gctcgacgac gcgacgctcg cggtgggcga
     1561 acccgcggcg gtgcgcgcgg ccatcgacgc gcaggcggga cgcggcggcg tcgataaagc
     1621 gctcctgaac acggcgcggc agcccggcgc gctcgtcggc ttcgcgggca acgtgccgga
     1681 gggcgcgttc aaagacctcg acacggggct gcccgaagtg gacaagagcc tcgcctcgat
     1741 ccgccgcttc cacggctcgg tcgggatgag cggcacgggc tttcagttgc agacgatcct
     1801 cgtgacgcag agcgcgcagg acgcctcgcg gctgcgcggg acgatagacg cggtgcgctc
     1861 ggtcgcgccc gcgctgatct cgatggcggg cgagcgcggc cagttggcga agagcgccat
     1921 ccagaacatc aaggtcacgg cgcagggcac ggacgtgcag ctccgcctcg acctcgcgca
     1981 gaacgaactg gccgccatca tgcgcgtcct ttaaaagccg tcaaccgtca accg
//
LOCUS       DBNGOH010001069         1890 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2176967, whole genome shotgun sequence.
ACCESSION   DBNGOH010001069 DBNGOH010000000
VERSION     DBNGOH010001069.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1890)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1890)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1890
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2176967"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1890)
                     /locus_tag="AC159P_20835"
     CDS             complement(<1..>1890)
                     /locus_tag="AC159P_20835"
                     /inference="COORDINATES: protein motif:HMM:NF017390.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HIR3697948.1"
                     /translation="VSRLFVPAATVPIMLRAGGGAASGAPSNANDAATRATLAREGAF
                     VEQETALPSHRSAGEARREAEWSPTENLREWVDDRFASLVPWLVVGWLMGVVLLLLRF
                     AGGFVGVRRLRRSARPVADELQRALARLAHRLRVTRAVRLCESALVEVPTVVGHLKPI
                     ILLPACALTGLKPAQLEAVLAHELAHIRRHDYLFNLLQTTVETLLFYHPAAWWLSRRV
                     RAEREHASDDLAVSATGDVLGYARALAALEQLRLTPAHAPALALAANGGSLMKRIQRL
                     VNLGPAHPRHRTRAPFAAAAALLLTLCGVIACAHALTPLPPAAAAEDGLTTATDAAAP
                     AASVAPAAKREVAFTFVNFPGNNIYSNVRLTYKTRKLMRLLKRHRISAVGFVNEGRLY
                     KNDGEVDEERVALLGEWLDAGHELGNETFRHTSLYDAGFAVWRADVEQGEKIMSELLA
                     KRGARLRYFSYPYLNTGENAQQKAVAEKYLRARGYQIHPVTIDNMDWLFNRAYIEALR
                     REDEPAAARIRAEYVPYMERMFEFTESYSREVVGREFPQVLMLTGGALNADCLDDLVA
                     MMKRRGYSFVTMEQAMKDEAYSLPDTYTGERGDSWIARWAVTKGLEYRDTEEVNLPEY
                     MQQYFA"
BASE COUNT          280 a          642 c          672 g          296 t
ORIGIN      
        1 gcgaagtact gctgcatgta ctcgggcagg ttcacctcct cggtgtctct gtactcaagc
       61 cccttcgtga cggcccagcg cgcgatccaa gagtcgccgc gctcgcccgt gtaggtgtcg
      121 ggcaggctgt aggcttcgtc cttcatggcc tgctccatcg tgacgaaaga gtagccgcgc
      181 cgcttcatca tcgccacgag atcgtcgagg cagtcggcgt tcagcgcgcc gcccgtgagc
      241 atgagcacct gcgggaactc gcgcccgacc acctcgcgcg agtagctctc ggtgaactcg
      301 aacatgcgct ccatgtaggg gacatactcg gcgcggatgc gcgcggctgc cggctcgtct
      361 tcgcggcgca gcgcttcgat gtaggcgcgg ttgaagagcc agtccatgtt gtcgatggtg
      421 acggggtgaa tctggtagcc gcgcgcgcgc aggtacttct cggcgacggc cttctgctgc
      481 gcgttctcgc ccgtgttcag gtacgggtaa ctgaagtaac ggagacgcgc gccgcgcttc
      541 gccagcagct ccgacatgat cttctcgccc tgctccacgt cggcgcgcca cacggcgaag
      601 ccggcgtcgt agaggctggt gtggcggaag gtctcgttgc cgagttcgtg gcccgcgtcg
      661 agccattcgc cgagcagcgc gacgcgctcc tcgtccacct cgccgtcgtt cttgtagagc
      721 cgcccctcgt tgacgaagcc gaccgcgctg atgcggtgcc gcttcaggag gcgcatcagc
      781 ttgcgcgtct tgtacgtcag acgcacgttg ctgtagatgt tattgccggg gaagttgacg
      841 aaggtgaagg ccacctcgcg cttcgcggcg ggggcgacgg acgcggcggg cgcggcggcg
      901 tccgtcgccg tcgttaagcc gtcttcggcg gcggcggcgg gcggcagcgg cgtgagcgcg
      961 tgggcgcagg cgatgacgcc gcagagcgtc agcagcagcg ccgccgccgc cgcgaagggc
     1021 gcgcgcgttc gatgtcgagg gtgtgcgggt ccgaggttga caaggcgttg gatgcgtttc
     1081 atgagtgaac ctccgttggc ggcgagcgcc agcgcgggcg cgtgggccgg cgtcaggcgt
     1141 agttgttcga gcgcggcaag cgcgcgggcg taacccagca cgtcgcccgt cgcggagact
     1201 gcgaggtcgt ccgaggcgtg ctcgcgctcg gcgcgcacgc ggcgcgagag ccaccacgcc
     1261 gcggggtggt agaagagcag cgtctcgacc gtcgtttgca gaaggttgaa gaggtagtcg
     1321 tggcggcgga tgtgcgccag ctcgtgcgcg agcacggctt cgagctgcgc gggcttgagg
     1381 cccgtcagcg cgcacgcggg gagcaggatg atcggtttga gatgtccgac caccgtcggc
     1441 acttcgacca gcgccgactc gcagagccgc accgcgcgcg tcacgcgaag gcggtgggcg
     1501 aggcgcgcga gcgcccgttg cagttcgtcg gcgacgggcc gcgcgctccg cctcagacga
     1561 cgcacgccca cgaagcctcc cgcgaagcgc agcaagagca gcacgactcc catcaaccag
     1621 ccgaccacca accacggcac cagcgaggcg aagcggtcgt caacccactc tctcaaattc
     1681 tccgtcggcg accactccgc ctcacgcctc gcctcgcccg ccgagcggtg cgaaggcaag
     1741 gccgtctcct gttccacgaa cgccccctcg cgcgcgagcg tggcgcgcgt cgccgcgtcg
     1801 ttcgcgttcg acggcgcgcc cgacgccgcg ccgccgcccg cacgcagcat gatgggcacc
     1861 gtcgccgccg ggacgaagag ccgcgacacg
//
LOCUS       DBNGOH010001070         4070 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1813482, whole genome shotgun sequence.
ACCESSION   DBNGOH010001070 DBNGOH010000000
VERSION     DBNGOH010001070.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4070)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4070)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4070
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1813482"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(197..937)
                     /locus_tag="AC159P_20840"
     CDS             complement(197..937)
                     /locus_tag="AC159P_20840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013033134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR3697949.1"
                     /translation="MKRPVLILGATSAIACGAAAAFAARGHALYLAGRDREELERIAS
                     DLRLRHQVEVLHGHFDAEDYAGHGAFVERVRAEMGGLHGVLLAFGYLSERAGARGAEE
                     GAATIARNLTGAVSVLEHCAAALESQDSGGFIIGVSSVAGDRGRQSNYLYGAAKGGLS
                     VYLQGLRNRLHPAGVRVLTVKPGFVDTRMTYGMPGLFLVARPASVGAAIVRALERGRD
                     VVYVPWFWRYVMLAVRLIPEPVFKRLKL"
     gene            complement(943..2283)
                     /locus_tag="AC159P_20845"
     CDS             complement(943..2283)
                     /locus_tag="AC159P_20845"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HIR3697950.1"
                     /translation="MEIHGWGRYPRGRSRVLRPERVREAVPPDEGSVIARGQGRSYGD
                     AAMSADGAVMLTERLNRFLAFDEETGVLRAEAGATLAEVLAVFLPRGWFPPVTPGTKF
                     VSLGGALAADVHGKNHHRDGTLGAHVTELELALADGTRRRCSPDREAELFRATVGGMG
                     LTGIITEVSLKLARVESAHVVVRHSAARDLDALLELLEDEEYDDRYSVAWVDSLARGR
                     ALGRGVLMTGRHATLAALPRDIAEPLAMKSPRRLGLPFDFPAWVLNPLSVSTFNELYF
                     RRQGARDVPFVADCESFFYPLDRVGDWNRLYGARGFVQYQCVLPPATARGALRLLFEE
                     LSRTRRASFLAVLKRFGPEGHGLLSFPTEGYTLALDLPVTGEDLFALLDRFDELVLEH
                     GGRVYLAKDTRLRPETFRAMYPRFAEWLRVKQRFDPEDRFSSDLSRRLRMGAQA"
     gene            complement(2487..3917)
                     /locus_tag="AC159P_20850"
     CDS             complement(2487..3917)
                     /locus_tag="AC159P_20850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014396711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HIR3697951.1"
                     /translation="MAKARVLIVDDEPSIAEWLSDTLAGWGYETEIAEDGAVGLTLAD
                     EFSPSVVITDVYMPNLDGFGLLRELRERHPETAVILLTGKGNVEMALNAIQEKGAYHY
                     FEKPVDASKLRVILKRAVETGEARRETDALRRELREHGAFGELVGSSESMRQIYSLIE
                     QVAPSSASVLITGESGTGKELVARTVHNLSPRRGAAFVAINCSAIPETLMESELFGHE
                     KGAFTGAASRRPGCFEMANGGTLLLDEIAEMPVALQAKLLRVLEDRKIRRLGGGQEIP
                     VDVRVLAATNRDPHDAVRRGSFREDLLYRLNVITIELPPLRRRREDIPLLAQHLVTQL
                     AERHGRPARHLSASALEVLKSHDWPGNVRELRNVVERAVIICSGEAIERLHLAPYPLE
                     QRQRARDEDTLTLPVGTPIEEVERQMIFRTLQKTGNDKTRAAERLQISRKTLHNKLRG
                     YRERGLLQDPDHARPLAASARDDDYD"
BASE COUNT          637 a         1319 c         1455 g          659 t
ORIGIN      
        1 gggtgttacg ggagccttgc aaacctgtgc gttaagagcg cgccaattta acacagaggg
       61 cacggaggtc tcacagagag cacggaggtt cgagtgtcgt tcaatcctct gtgcgctctg
      121 tgagacctcc gtgccctctg tgtcgagatg tagcttgccc tgtactcaag cgaggagcgg
      181 ttataaggct tcggttctat agtttcagcc tcttgaacac aggctccgga atcagcctga
      241 cggcgagcat gacgtagcgc cagaaccagg gtacgtagac cacgtcgcgc ccgcgttcga
      301 gcgcgcggac gattgcggcg ccgacgctcg cggggcgcgc gacgaggaag aggccgggca
      361 tgccgtacgt catccgcgtg tccacgaagc cgggcttgac ggtgagcacg cggacgcccg
      421 ccgggtggag gcggttgcgc aaaccttgca ggtagacgct gaggccccct ttagcggcgc
      481 cgtagaggta gttgctctgc cgcccgcggt cgcccgcgac cgacgagacg ccgatgatga
      541 agccgccgct gtcctgactc tccaacgcgg cggcgcagtg ttcgaggacg gaaaccgcgc
      601 ccgtgaggtt gcgcgcgatg gtcgccgcgc cttcctcggc gccgcgcgcg cccgcacgct
      661 cggacagata gccgaaggcg aggaggacgc cgtgcagccc gcccatctcc gcgcggacgc
      721 gctcgacgaa ggcgccgtgg cccgcgtagt cctcggcgtc gaagtggccg tggaggactt
      781 cgacttgatg acgaagacga aggtcggagg caattctttc gagttcttcc cggtcgcgcc
      841 cggcgaggta gagcgcgtgg ccccttgcgg cgaaggccgc cgccgcgccg cacgcgatag
      901 cagaggtcgc gccgaggatg agcacgggcc gcttcatcgc tttcacgcct gcgcgcccat
      961 ccggagcctg cgcgagaggt cggagctgaa acggtcttcc gggtcgaagc gctgcttgac
     1021 gcgcagccac tcggcgaagc gcgggtacat cgcgcggaag gtctcgggcc gcagccgcgt
     1081 gtctttggcg aggtagacgc gcccgccgtg ctccagcacg agttcgtcga agcggtcgag
     1141 gagcgcgaac aagtcttcgc ccgtcaccgg caggtcgagc gcgagcgtgt agccttcggt
     1201 cgggaaagag agcaggccgt gcccctcggg gccgaagcgc ttgagcacgg cgaggaagct
     1261 cgcgcggcgc gtgcgcgaca gctcttcgaa cagcagccgc aaggccccgc gcgccgtcgc
     1321 gggcggcagc acgcattgat actggacgaa gccgcgcgcg ccgtacaggc ggttccagtc
     1381 gccgacgcgg tcgagcgggt agaagaagct ctcgcagtcc gcgacgaagg gcacgtcgcg
     1441 cgcgccctgc cggcggaagt agagttcgtt gaaggtcgag accgacagag ggttcagcac
     1501 ccacgcaggg aagtcgaagg ggagaccgag cctgcgtggg gatttcatcg ccagcggctc
     1561 cgcgatgtcg cgcggcagcg ccgcgagcgt cgcgtgccgg cccgtcatca ggacgccgcg
     1621 cccgagtgcg cgcccgcgcg cgagcgagtc aacccaggcg acgctgtaac ggtcgtcgta
     1681 ctcttcgtct tcgagcagtt cgagcagcgc gtcgaggtcg cgcgcggcgc tgtgccgcac
     1741 gacgacgtgc gcgctctcga cgcgcgccag cttcagagag acttcggtga tgatgcccgt
     1801 caggcccatg ccgccgacgg tggcccggaa gagttccgct tcgcggtcgg gcgagcagcg
     1861 gcggcgcgtg ccgtcggcga gtgccagctc aagctccgtg acgtgcgccc cgagcgtgcc
     1921 gtcgcggtgg tggttcttgc cgtgcacgtc cgccgccagc gcgccgccga gcgagacgaa
     1981 cttcgtgccc ggcgtgacgg gcgggaacca gccgcgcggg aggaagacgg cgagcacttc
     2041 ggcgagcgtc gcgcccgcct cggcgcgcag cacgcccgtc tcctcgtcga acgcgaggaa
     2101 gcggttcagc cgctccgtca acatcaccgc gccgtcggcg gacatcgccg cgtcgccgta
     2161 gctgcggccc tgcccgcgcg cgatgacgct gccctcgtcg ggcggcaccg cctcgcgcac
     2221 gcgctcgggc cgcaagaccc gcgagcgccc gcgcgggtag cgtccccagc cgtgaatctc
     2281 catcgtggcg gttcgagacc taagtgaagc gggcagcagg cagcgggcag caggcagtta
     2341 atccaggaac agattcgaga tccggaattg catatctccg gcaggtcgca gcccgcaacg
     2401 gagacttcga ttcttaagga gactttgatt cttaaattga attgactgtc ttctgccttt
     2461 actgcccgct gcccgctgcc ccctgctcag tcgtagtcgt cgtcgcgcgc ggaggcggcg
     2521 agcgggcgcg cgtggtcggg gtcttgcagg agtccgcgct cgcggtagcc gcggagcttg
     2581 ttgtggagcg tcttgcggct gatctgcaac cgctcggcgg cgcgcgtctt gtcgttgccg
     2641 gtcttctgga gagtgcggaa gatcatctgc cgctcgacct cttcgatggg cgtgccgacg
     2701 gggagcgtca gggtgtcttc gtcgcgtgcg cgctgccgct gttcgagcgg gtagggcgcg
     2761 aggtggaggc gctcgatggc ctcgccggag cagatgatga cggcgcgctc gacgacgttc
     2821 cgcagctcgc gcacgttgcc gggccagtcg tgcgacttga ggacttcgag cgccgaggcg
     2881 ctgaggtgtc gggccgggcg cccgtgccgc tcggcgagct gcgtgacgag gtgctgcgcg
     2941 agcagcggga tgtcttcgcg gcggcggcgc aaagggggca gctcgatggt gatgacgttg
     3001 aggcggtaga gcaggtcttc gcggaacgag ccgcggcgca cggcgtcgtg cgggtcgcgg
     3061 ttggtcgcgg cgagcacgcg cacgtccacg gggatttcct gcccgccgcc gagccttcga
     3121 atcttgcggt cttcgagcac gcgcaggagc ttcgcctgaa gcgcgaccgg catctcggcg
     3181 atctcgtcga gcaggagcgt cccgccgttc gccatctcga agcagcccgg cctgcgcgag
     3241 gccgcgccgg tgaaggcgcc cttctcgtgg ccgaacagct cggactccat caacgtctct
     3301 ggaatcgccg agcagttgat cgccacgaac gccgcgccgc gccgcgggct gaggttgtgc
     3361 acggtgcgcg cgaccaactc cttgcccgtg cccgactcgc ccgtgatgag cacggaggcc
     3421 gaggagggcg cgacctgttc gatgagcgag tagatttggc gcatcgactc ggacgagccg
     3481 accagctcgc cgaaggcgcc gtgctcgcgc agctcgcgcc ggagcgcgtc ggtctcgcgc
     3541 cgcgcctcgc ccgtttcgac cgcgcgcttg aggatgacgc gcagcttcga ggcgtccaca
     3601 ggcttctcga agtagtggta ggcgcccttc tcctgaatcg cgttgagcgc catctcgacg
     3661 ttgcccttgc cggtgagcag gatgacggcg gtctcggggt gccgctcgcg cagctcgcgc
     3721 agcaggccga agccgtcgag gttgggcatg tacacgtcgg tgatgacgac cgagggcgag
     3781 aactcgtcgg cgagcgtgag gccgacggcg ccgtcctccg cgatctcggt ctcgtagccc
     3841 cagccggcca gcgtgtcgct caaccactcc gcgatggagg gttcgtcgtc aacgatgagg
     3901 acacgcgcct ttgccatgag tcgagtttag cacaggaaca gtaggcagta agcagtggcg
     3961 gtaggcagtc aagaaatatc ggggttgaat tctcgaactt cgaatcccaa gtccgaaacc
     4021 tgtaatttcg aatctcgaat ctgttttgtc ttgactgcct accgcctact
//
LOCUS       DBNGOH010001071         2236 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_529541, whole genome shotgun sequence.
ACCESSION   DBNGOH010001071 DBNGOH010000000
VERSION     DBNGOH010001071.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2236)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2236)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2236
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_529541"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..839)
                     /locus_tag="AC159P_20855"
     CDS             complement(<1..839)
                     /locus_tag="AC159P_20855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697952.1"
                     /translation="MPTPKRVFGAGVSWALKLGLAALLLASAVFILASSQPQSAQQRF
                     REAAVKVTGIASNGTSYTITADGPLTRAQTFQTEGWFHVIIVNGQTDLGRGAAGGVRL
                     QRISESLEILIPVKPGASVTVNPRGNRLDLSVEGGSAAAVSRVERAAPQQAAPTETPR
                     AQGERATREAALERAEREAAKRNERAQQLTGEPSAASKKGQAIEAPAATAPLTTNSTA
                     PVNAAPQAGAGQANDAAAALAAEAEARKNSEAPTQASAAAAQLEGGEGGGLSGTLFPP
                     PAL"
     gene            complement(1002..1778)
                     /locus_tag="AC159P_20860"
     CDS             complement(1002..1778)
                     /locus_tag="AC159P_20860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012283135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR3697953.1"
                     /translation="MTTKPAAINTRKDADEETPEAGDRKLNLLVIEDDEKILEMISEH
                     FSRAGFSVQTAEDGLSGVQIAMNERPDVILLDLMLPKMDGLAVCREVREKAPYIPILM
                     LTAKDDVVDKVLGLEMGADDYITKPFSLRELEARIKSVMRRVRSAARTETASEEAPIM
                     RGRLRIDPSKREVMIGDRQVELTPKEFELLRLFAANPGRVFPRKYLLEKIWDYSYEGY
                     DRTIDSHINRLRAKIEENPENPQMVLTVWGIGYKFSDEQR"
BASE COUNT          327 a          758 c          723 g          428 t
ORIGIN      
        1 atcagcgccg gcggcgggaa gagtgtgccc gacaggccgc cgccctcgcc gccctcgagt
       61 tgcgcggctg ccgccgacgc ctgcgtcggt gcttcggaat ttttccgcgc ctcggcttcc
      121 gctgcgagcg ccgccgccgc gtcgttagcc tgccccgcgc ccgcctgcgg agcggcgttg
      181 acgggagccg tgctgtttgt cgtcaacggc gcggtcgcgg cgggggcttc gatggcctgc
      241 cccttcttcg aggcggcaga cggctcgccc gttaactgtt gcgcgcgttc gttgcgcttc
      301 gccgcctccc gctcggccct ctcaagcgcg gcctcgcgcg tcgcccgttc tccctgcgcg
      361 cgcggcgtct cggtcggcgc ggcctgttgc ggtgcggccc tctcgacacg actcacagcg
      421 gcggcgcttc caccttcgac ggagaggtcg aggcggttgc cgcgcgggtt caccgtcacg
      481 ctcgcgcccg gcttgacggg gatcaggatt tcgagcgact cgctgatgcg ctgaaggcgc
      541 acgccgcccg ccgcgcccct gccgaggtcg gtctggccgt tgacgatgat gacgtggaac
      601 cagccctcgg tctggaaggt ctgcgcgcgc gtgagcgggc cgtcggcggt gatcgtgtac
      661 gacgtgccgt tcgaggcgat gcccgtcacc ttcaccgccg cctcgcggaa gcgctgctgc
      721 gcgctctgcg gctgcgacga cgcgaggatg aagaccgccg acgccaggag cagcgcggcc
      781 agcccgagct tcaacgccca cgacacgccg gcgccgaaga ctcttttcgg ggtcggcaaa
      841 acgctattct tccggaaacg ttccgtgtgc atggtttttc ttccggcgag accgtccaag
      901 acaagggcgg tgaggagtaa ggtcggtttg ttcgggttgg agggcgcgag cggggcgtgt
      961 gcccttcgcc cttctctcag gagagagagc agccttgaag gttatcgctg ctcgtcgctg
     1021 aacttgtagc cgatgcccca gacggtcagc accatctgcg ggttctccgg gttctcttcg
     1081 atcttcgcac gcaggcggtt gatgtgcgag tcgatcgtgc ggtcgtagcc ctcgtaggag
     1141 tagtcccaga ttttctccag gaggtatttg cgcgggaaga cgcgaccggg gttggccgcg
     1201 aacaggcgga gcagttcgaa ctccttcggc gtgagttcca cctgccggtc gccgatcatc
     1261 acctcgcgct tcgaggggtc gatgcgcagc cgcccgcgca tgatcggcgc ctcttcgctc
     1321 gccgtctcgg tgcgcgccgc cgagcggacg cggcgcatca cgctcttgat ccgcgcttcc
     1381 agctcgcgca gcgagaacgg cttggtgatg tagtcgtcgg cccccatttc gaggccgagc
     1441 accttatcga ccacgtcgtc cttcgccgtc agcatcagga tcgggatgta cggcgccttc
     1501 tcgcgcacct cgcggcagac ggcgaggccg tccatcttcg gcagcatcag gtcgagcagg
     1561 atcacgtcgg gccgctcgtt catggcgatc tgcacgccgg agagtccgtc ctccgccgtc
     1621 tgcacgctga agcccgcgcg cgagaagtgc tcggagatca tctccagaat cttctcgtcg
     1681 tcttcgatga cgagcaggtt gagcttgcgg tcgccagctt ctggcgtctc ctcatccgcg
     1741 tcttttctgg tgttgatggc agccggtttt gtggtcatcg caggccgtgt cgttcaagga
     1801 agcgagccgg ggcggcccgc caaccgaggc gccgacctga gggcgggcgg cgctcggacg
     1861 taggaaatat aacaggttag cgagagcaaa ctcaagcacg aagttattca cgggcgcccg
     1921 cccgcgccca cgcccggcag cgtacagacg aaagcacgtc aaccatttac gggcctctcg
     1981 tccggtacgc atcaacgccg agcgcccgcc gcccgttcct cccgccccgc ccttccgtaa
     2041 tttgcccttc agcattccgg catttcaaag tttacccttg tgtgctattt aaaactccga
     2101 gaccggctca gcagtcggtg gctgattgaa ccaacgccgt ctttgccgcc cttcaccgac
     2161 caccggccac cggcctctga acacttttac gacgaggaga gttatgaatc acgacgagag
     2221 acaggcactc atcgca
//
LOCUS       DBNGOH010001072         2787 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2361743, whole genome shotgun sequence.
ACCESSION   DBNGOH010001072 DBNGOH010000000
VERSION     DBNGOH010001072.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2787)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2787)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2787
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2361743"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(90..1430)
                     /locus_tag="AC159P_20865"
     CDS             complement(90..1430)
                     /locus_tag="AC159P_20865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HIR3697954.1"
                     /translation="MKFIRYSKFKGFDVSGVSLGDLMEMLQDSLLQSGYDDDYYWTRR
                     RQEPDTSLDALRQALLQALLDMGELDARDVEQMLEENKGQFKGSQLEELINRLIERLV
                     EEGYLKLSEEPRRADERRRRRGQPGQGETGEPLPRDVKFEVTEKGLDFLGYKTLRSLL
                     GSMGKASVGRHDTQELATGVEAAQASKPYEFGDTINLDVNTTLMSAIKREGLGVPLNL
                     EYADLHVHQCDYQSSCATVVMLDCSHSMILYGEDRFTPAKKVALALAHLIRTQYPGDS
                     LKVVLFHDDAEELPLARLATTQVGPYHTNTAEGLKLARRLLQAQRKEMKQIVMVTDGK
                     PTACFLDAPAPATVGGRSRAHLFGELHGGRRLYKNSMGGDPVVMEATFREVQACRKAG
                     VMINTFMLASDYYLVEFVKQMTQMTSGKAYFATPGNLTQFVLIDFLKRKKSRVR"
     gene            complement(1438..2223)
                     /locus_tag="AC159P_20870"
     CDS             complement(1438..2223)
                     /locus_tag="AC159P_20870"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017690283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HIR3697955.1"
                     /translation="MEERIKASLLEIERGEGVHIFYACESGSRAWGFPSADSDYDVRF
                     LYLRPTDWYLSVDVEKRRDVIERPITDELDVSGWDIRKALMLLRKSNPPLLEWLGSPI
                     VYQEKFGMADKLRNLLPEYYSPSACVHHYLHMAQGNYREYLKGDVVWVKKYFYVLRPL
                     LAIKWIEEWDEVVPMEFQALIERTVEDGELRAIIADLIEQKKDGRELDRGPAIPRLSE
                     FVRAELERLEKKSFEYAPSKTSTDKLNEVFRASLSEAWGSQQR"
     gene            complement(2227..>2787)
                     /locus_tag="AC159P_20875"
     CDS             complement(2227..>2787)
                     /locus_tag="AC159P_20875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase"
                     /protein_id="HIR3697956.1"
                     /translation="GVSQRLPISVTESVVSNAERRALTTDETEIVPRVSDVYAAVPSM
                     TGKMELEYEGEQVGAAKIARDLIKRAAGEIFEGYFVGVDFTPAVRWFDEGNTLRLADT
                     ASAEECVTLLESVPDLIETVLVPFDFKREDRGRLVSACEFALEGLYAQNKISRNEEGG
                     YTAVTKARKDRRGMIYDDLTETGRYS"
BASE COUNT          475 a          898 c          832 g          582 t
ORIGIN      
        1 aatttcaaat ccgaaaatcg ttttctccgt gtctgctgtg tctcctccgt gccctctgtg
       61 ttgaatcgaa acttgccgaa tgctcaacgt cagcggacgc ggctcttctt gcgtttgagg
      121 aaatcgatca gcacgaactg cgtgaggttg ccgggcgtgg cgaagtaggc tttgccgctc
      181 gtcatctgcg tcatctgctt gacgaactcg acgaggtagt agtccgacgc cagcatgaag
      241 gtgttgatca tcacgcccgc cttgcggcac gcctgcacct cgcggaaggt ggcctccatc
      301 acgacggggt cgccgcccat cgagttcttg taaagtcttc gcccgccgtg cagctcgccg
      361 aagaggtgcg cgcggctccg gcccccgacg gtcgcgggcg cgggcgcgtc gaggaagcag
      421 gcggtgggct tgccgtccgt caccatcacg atctgcttca tctccttgcg ctgcgcttgc
      481 agcagacggc gcgcgagctt cagcccctcg gcggtgttcg tgtggtaggg gccgacctgc
      541 gtcgtggcga gccgcgcgag cggcagctcc tctgcgtcgt cgtggaagag cacgaccttc
      601 agagagtcgc cggggtactg cgtgcggatg aggtgcgcga gcgcgagcgc gaccttcttg
      661 gcgggcgtga agcggtcttc gccgtagagg atcatcgagt gcgagcagtc gagcatgacc
      721 acggtcgcgc acgacgattg atagtcgcat tgatggacgt gcaggtcggc gtattcgagg
      781 ttgagcggca cgcccagccc ctcgcgcttg atggccgaca tcagcgtcgt gttcacgtcg
      841 aggttgatcg tgtcgccgaa ctcgtaaggc ttgctggcct gcgccgcctc gacgcccgtc
      901 gccagctcct gcgtgtcgtg ccgcccgacc gaagctttgc ccatgctccc cagcaggctc
      961 cgcagcgtct tgtagccgag aaagtccaga cccttctccg tcacctcgaa cttcacgtcg
     1021 cgcggcagcg gctcgcccgt ctcgccctga ccgggctgcc cgcgcctccg cctccgctcg
     1081 tccgcccgtc gcggctcctc cgacagcttc agataacctt cctcgaccag ccgctcgatc
     1141 aaacggttaa tcaactcttc gagctgcgac cccttgaact gccccttgtt ctcttcgagc
     1201 atctgctcca cgtcgcgcgc gtccagctcg cccatgtcca agagcgcctg caagagcgcc
     1261 tgccgcagcg cgtccagcga ggtgtcaggc tcctgccgcc gccgcgtcca gtagtagtcg
     1321 tcgtcgtacc ccgactggag cagcgagtcc tgcaacatct ccatcagatc gcccaaggag
     1381 acgcccgaca cgtcgaagcc cttgaacttg gaatagcgga tgaacttcat tcggagatca
     1441 cctttgctga ctgccccatg cttcgcttaa tgaagcccga aagacctcgt tcaatttatc
     1501 cgtcgatgtc tttgacggcg cgtactcgaa cgatttcttt tcgagccgtt ccagctctgc
     1561 cctcacaaac tcgctgagcc gggggattgc aggcccacga tcaagttcac gaccgtcctt
     1621 cttttgttcg atcaaatcag cgatgatcgc tctgagttcc ccgtcttcga cggtgcgctc
     1681 gatcaacgcc tgaaactcca tcgggacgac ctcgtcccac tcctcgatcc atttgatcgc
     1741 cagcagcggc ctcaagacat aaaaatactt cttgacccag acgacatcgc ctttcagata
     1801 ctcccggtaa ttaccttgcg ccatgtgtaa gtagtgatgg acgcaggcgc tcggcgaata
     1861 gtattccggc aacaagtttc tcagcttgtc ggccatcccg aacttttcct gatagacgat
     1921 gggggagcca agccattcca gtaacggcgg gttggatttc ctgagcagca tcagagcctt
     1981 tctgatgtcc cacccgctga catcaagctc gtccgtaatc gggcgttcga tcacgtcgcg
     2041 cctcttttca acgtccaccg acagatacca gtcggtcggt cggaggtaga gaaacctcac
     2101 gtcgtaatcg ctgtccgccg aaggaaagcc ccacgcccga ctgccggact cacaggcgta
     2161 aaaaatgtgc acgccttcgc cgcgctcgat ctcgagtaac gacgccttga tgcgctcttc
     2221 catcgcttaa ctgtaacgcc cggtctcggt caggtcgtcg tagatcatgc cgcggcggtc
     2281 cttgcgcgct ttggtgacgg cggtgtagcc gccctcttcg ttgcggctga ttttgttctg
     2341 ggcgtagaga ccttcgaggg cgaattcgca ggccgagacg agccggcccc ggtcttcgcg
     2401 cttgaagtcg aaggggacga ggacggtctc gatgaggtcg gggacggatt cgagcagcgt
     2461 gacgcactct tccgccgagg ccgtgtcggc gaggcggagc gtgttgcctt cgtcgaacca
     2521 gcgcacggcg ggcgtgaagt ccacgccgac gaagtagcct tcgaagattt cgccggcggc
     2581 gcgcttgatg aggtcgcgcg cgatcttggc ggcgccgacc tgctcgccct cgtattcgag
     2641 ttccatcttg ccggtcatcg aggggacggc ggcgtacacg tcggagacgc gcgggacgat
     2701 ctccgtttcg tcggtcgtga gcgcgcgccg ctcggcgttc gagacgacgg attcggtgac
     2761 ggagatgggg aggcgctgcg agacgcc
//
LOCUS       DBNGOH010001073        10100 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1995939, whole genome shotgun sequence.
ACCESSION   DBNGOH010001073 DBNGOH010000000
VERSION     DBNGOH010001073.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 10100)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10100)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10100
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1995939"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..590
                     /locus_tag="AC159P_20880"
     CDS             <1..590
                     /locus_tag="AC159P_20880"
                     /inference="COORDINATES: protein motif:HMM:NF012523.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CatA-like O-acetyltransferase"
                     /protein_id="HIR3697957.1"
                     /translation="DNPYFNVCTRLDVTALLALLGARPGVSVSLTYHYFALRVANETE
                     PFRYRLRDGRVLVHEVIHGGTTVLLPNETFAFAYFSYEEDFGKFIAAAQRAVNLLRAG
                     GDVFRPQANDDMIHFTTLPWVAFTSFAHARNWGTGDSVPKIAFGKFTKEHGRVLLPIS
                     VEVHHALMDGLHVGRYLARLEEALAEPETYLRASG"
     gene            complement(715..3840)
                     /locus_tag="AC159P_20885"
     CDS             complement(715..3840)
                     /locus_tag="AC159P_20885"
                     /inference="COORDINATES: protein motif:HMM:NF051698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR3697958.1"
                     /translation="MVSKIRLCLVVAAFVLASAGSVLGQETTGNIEGYVSDPTGARVA
                     GATVRITGTSFDRTASTDEEGFFRVLQVPPGLYTITTGAPSFRTAKQERVEVVLGNTT
                     NANVALEVGEVTEEVLITAADAAPIDTNNSRIQTNITEKKIELTPRGTNFTSVLQVSP
                     AVRVEPLSGGFQIDGASGSENTFIIDGQEVTNFRTGALNVNNNIPFEFVQEVQVKSSG
                     FEAEFGGATGGVINVATKRGDNEYHGSIGTQFEVSRLAAGTRPVQSANEQTLTYFHFP
                     RDEYTRFFPSATFSGPAIKDRLWFFSSYSIQDVPTRRSYIYPNNTGVTYESRDRRDYG
                     FLRLDGNVSDTLQLSGTYTYNPLKRNGILPTFNTIANAGTAAAAVNPRNQSFRGGRQP
                     ASNVGGSAVWTPTGNLVISARAGRAYLNEKLDNYAVPNAIRILCRGTGNVGLPGVTGA
                     GQCVSGFQNLTGNSLETKDISIRQTFDADASYVVSGLGGRHLFKGGYQLNRLSNDVLT
                     GNVGVGTAVLNFGQTFDDAYGNPRGAGPGEFGYGFLQQFGEVGKVSSKNEGIYIQDTW
                     QPTSRLTLNLGLRTERENVPPFNDGPGLSFGFGAKIAPRLGFAYDVTGTGKFKVFASF
                     GRFYDRFKYELPRGSFGGNIFTNDYFVITNPNWNATTFADSVARRFLQLNFRVPSNVR
                     VNDPAFQETFGDLPSTVDPDLKPVRQTEYTAGVEYGLGSDIVLAARYTHKQIDRTIED
                     VGIPDSLGNETFFIANPGEGITATSLVTGVPATPKPQRDYDAVEFRLDKRFARNYFLN
                     ASYTWSRLFGNYGGLASADERAFAAIGGSDTPNVGLQGRLAPNVTRAFDLPFESFTPD
                     GEPNNGRLPTDRPHVFKFFGGYTWNWFGRARNTTDFYASFIGTSGTPLTTNVDILDVV
                     VVMNGRGDLGRTEKFTQTDLGLTHRYRFGSSERFAMVFEANVLNLFNEANELDRLTLI
                     SSAIFGPSQLTGAAGASRTDGIRAIFNGGTRDEINALLGSTTFPRDARFNTPNAFQGP
                     RQVRFGFRFQF"
     gene            4243..5304
                     /locus_tag="AC159P_20890"
     CDS             4243..5304
                     /locus_tag="AC159P_20890"
                     /inference="COORDINATES: protein motif:HMM:NF018867.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HIR3697959.1"
                     /translation="MLRKNRGLSRRVVLGVVLAGWLGGVGAHPTDAHTSARRAAQGRN
                     AISGHVFDTGRRPVEGVYVELLDEVDTVLRKVRTDASGRYAFSNLSDGTFQVRVLTHG
                     TNLVGQTRRVSITNFMGGAAGQSYEEHFTLAASKAPGDTSTAVAASSPGLVFVQEGVP
                     ETAKAAYERGVRELEREGGNKASALAELKKAVELFPTYYLALEQLGAEYVRQQQYEPA
                     RAALLKAVEVNPRGYKSLYALGVAQYNLKQKSEATESLRRSVELNPSSVNSQLWLGIV
                     LFKDGKAVQAEPHLKRAHELGEKRVPDVHMYLAQLYSDTKRYAQAADELEHFLKEAPA
                     ARDAENIRRLIKQLREKAR"
     gene            5533..5847
                     /locus_tag="AC159P_20895"
     CDS             5533..5847
                     /locus_tag="AC159P_20895"
                     /inference="COORDINATES: protein motif:HMM:NF021182.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HIR3697960.1"
                     /translation="MGENQWFFGLPIPHPDDCELMQFTGVIDNNGTEIYEGDVVAYYQ
                     NGLREVSQIIFSDGMFRMEKSCVDLQNWVDRNELEVIGNIYSTPALSQNGAAAGSEAD
                     ES"
     gene            complement(6109..6480)
                     /locus_tag="AC159P_20900"
     CDS             complement(6109..6480)
                     /locus_tag="AC159P_20900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697961.1"
                     /translation="MYKVMTRVALALLLCVLAAAPSLAKVKSRHITIGQDFTVGGTAV
                     KAGTYIFSFDTGTNELTVSDKKSKQVLARVAATTGERSSNSRTVDVKLSDDSARTLVS
                     VAFAGEKQAFVVGNSTAAGSR"
     gene            6980..7342
                     /locus_tag="AC159P_20905"
     CDS             6980..7342
                     /locus_tag="AC159P_20905"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3697962.1"
                     /translation="MPNILLIEDMPLLRDLLRDALAGYVCHAVRTAEEGLEILARVPF
                     DAVITDVGLPGMGGAELLRTLRAIRPRTPVIVITGGDEALREEDFIDLGASAYLQKPF
                     TVDEFMQAVEQALGRRRA"
     gene            7443..8042
                     /locus_tag="AC159P_20910"
     CDS             7443..8042
                     /locus_tag="AC159P_20910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697963.1"
                     /translation="MKRLIFNVAATMLSTLTFACPDGDAEPHTPSPTPAPGKYEKAIR
                     NLEEGNDNQVVEEAIRELYAAGTDAFPDLFAHFGDQRKASVKHFGAKALQCAPGVEPC
                     PPYHPTIGEACFDILGGQVEGNWPKGARGYYTLTAGNAKAWWEARRGMSLKELQLEAA
                     RSSLAAARKDRAAGKASKDLVKFLEEHLKDVREGRCCPE"
     gene            8082..9146
                     /locus_tag="AC159P_20915"
     CDS             8082..9146
                     /locus_tag="AC159P_20915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697964.1"
                     /translation="MRIYEDYAQSPDGWNITSTLKLEDDGRFQYQESWTDFTNATLGV
                     TAEGSWRRDADAIVLHPLRVEGSVGGWESAKERKAVERGDILDFGGGLTLRIPPEREQ
                     VFPVRNTGTKPLTVVLEPWGTRHVVAPGERARVVARGPGGMGELEIVRGEDEIVVYGW
                     SESRVAVVTEPKPLGAGATSRAPGTKKTVHTVKAPVAPLQPVRVAEPEIARVEPLAPS
                     PELAAHIRQWIDELPTEGLRFRLQRVCQELDALPLHGTLIYLWALRPDGVILCIDHEA
                     FGHPAEPESDPLTVYAVLAQGARTHPELGELLPPRPDGVRRCELCAGAGWTKAKPPAQ
                     GSSICFRCNGLGWYVAGTPR"
     gene            complement(9177..9857)
                     /locus_tag="AC159P_20920"
     CDS             complement(9177..9857)
                     /locus_tag="AC159P_20920"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006678079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3697965.1"
                     /translation="MAMHVRALFTHDARSRLLFVNEPGGDVPAPRLFVDRTREGNLWR
                     LRADLPETLAEELEALCADEPVGQEFQREPRHVEAYVRLLDGHAPVRRRWMGPAYHFT
                     RYFEPSRPLLAVTETDAGLLRGGFEELVAELPDGQPFVALVEEGRAVSVCRSVRITTA
                     AHEAGVETLPDFRGRGYAKDVVAGWARLVHALGAVPLYSTSWENTASQAVAKKLGLTA
                     YGADWHIT"
BASE COUNT         1790 a         3092 c         3486 g         1732 t
ORIGIN      
        1 acgacaaccc ttacttcaac gtgtgcaccc ggctcgatgt caccgcgctc ctcgcgctgc
       61 tcggcgcacg ccccggcgtc agcgtgtctt tgacatacca ctacttcgcg ctgcgggtcg
      121 ccaacgagac cgagccgttc cgctatcgcc tgagagacgg gcgggtgctg gttcacgaag
      181 tgattcacgg cgggacgacc gtgctcctgc cgaacgagac cttcgccttc gcgtacttca
      241 gctacgagga agatttcggg aagttcatcg ccgcggccca gcgcgcggtc aacctcctgc
      301 gcgcgggggg agatgtcttc aggccgcagg ccaacgacga catgattcac ttcacgacgt
      361 tgccgtgggt cgcgttcacg agcttcgcgc acgcgcggaa ctggggcacg ggcgactcgg
      421 tgccgaagat cgccttcggg aagttcacga aggagcacgg gcgcgtcctg ctgccgatct
      481 ccgtcgaggt gcatcacgcg ttgatggacg gcctgcacgt gggccgctac ctggcccgac
      541 tcgaagaggc gctggccgag ccggagactt acctccgtgc gtcgggttga tcgcggcggg
      601 gacggctccg tgcgcgggtg ttcgcgccgc gcgccttcgc cttgcgcgac gcatgaaaaa
      661 aaaggcccgc ccggatgtcc gggcgggcct tcatagttgg tacaggagca gttcctagaa
      721 ctggaacctg aagccgaagc gaacctggcg cgggccctgg aaggcgttcg gcgtgttgaa
      781 gcgcgcgtcg cgcgggaagg tggtcgagcc gagcagcgcg ttgatctcgt cgcgcgtccc
      841 gccgttgaag atggcgcgga tgccgtccgt tctcgaagcg cccgccgccc cggtcaactg
      901 gctggggccg aagatggccg acgagatgag cgtcagacgg tccagctcgt tcgcctcgtt
      961 gaagaggttc aggacgttgg cctcgaagac catcgcgaag cgctcgctgc tgccgaaccg
     1021 atacctgtgc gtcaggccga ggtcggtctg cgtgaacttc tccgtccggc ccagatcgcc
     1081 cctgccgttc atgacgacca ccacgtcgag gatgtccacg ttggtggtga gcggcgtgcc
     1141 gctcgtgccg atgaaggagg cgtagaagtc ggtcgtgttg cgggcgcgac cgaaccagtt
     1201 ccaggtgtag ccgccgaaga acttgaagac gtgcgggcgg tcggtcggga gccgcccgtt
     1261 gttcggctcg ccgtccggcg taaaggactc gaacggaagg tcgaaggcgc gcgtcacgtt
     1321 cggggcgagg cggccttgca gcccgacgtt cggcgtgtcg gagccgccga tggcggcgaa
     1381 ggcgcgctcg tcggcgctcg ccagaccgcc gtagttgccg aacaggcggc tccacgtgta
     1441 gctcgcgttg aggaagtagt tccgcgcgaa ccgcttgtcg aggcggaact cgaccgcgtc
     1501 atagtcgcgc tgcggcttcg gcgtcgcggg cacgcccgtg acgagcgagg tggcggtgat
     1561 gccctcgccg gggttggcga tgaagaaggt ctcgttgccc agcgagtcgg ggatgcccac
     1621 gtcttcgatg gtgcggtcga tctgtttgtg cgtgtagcgt gcggcgagga cgatgtcgct
     1681 gccgaggccg tactcgacgc cggcggtgta ctcggtctgc cgcacgggct tcaggtcagg
     1741 atcgacggtg ctcggcaaat cgccgaacgt ctcctggaag gccgggtcgt tgacgcgcac
     1801 gttggacggg acacggaagt tgagttggag gaagcgccgc gcgaccgagt cggcgaaggt
     1861 ggtggcgttc cagttcgggt tggtgatgac gaagtagtcg ttggtgaaga tgttgccgcc
     1921 gaaagacccg cgcggcagct cgtacttgaa gcggtcgtag aagcgaccga agctggcgaa
     1981 gaccttgaac ttgccggtgc cggtcacgtc gtacgcgaag ccgaggcgcg gcgcgatctt
     2041 ggcgccgaag ccgaaagaga ggcccggccc gtcgttgaac ggcgggacgt tctcgcgctc
     2101 ggtgcgcagc ccgaggttga gcgtcaggcg gctcgtgggc tgccaggtgt cctggatgta
     2161 gatgccttcg ttcttgctgc tgaccttgcc gacctcgccg aactgttgca ggaagccgta
     2221 gccgaactcg cccggcccgg cgccgcgcgg gttgccgtag gcgtcgtcga acgtctgccc
     2281 gaagttcaag accgcggtgc cgacgccgac gttgcccgtc agcacgtcgt tcgagaggcg
     2341 gttcaactgg tagccgccct tgaagaggtg gcgaccgccg aggccgctga cgacgtagct
     2401 ggcgtccgcg tcgaaggtct ggcggatcga gatgtctttc gtctcgagcg agttgcccgt
     2461 caggttctgg aagccgctga cgcactggcc cgcgccggtg acgccgggca ggccgacgtt
     2521 gccggtgccg cggcagagga tgcggatggc gttcggcacg gcgtagttgt cgagcttctc
     2581 gttgaggtag gcgcggcccg cgcgggcgct gatgacgagg ttgcccgtcg gcgtccagac
     2641 ggcgctgccg ccgacgttcg aggcgggctg gcgaccgccg cggaagctct ggtttctggg
     2701 gttcacggcg gcggcggcgg tgccggcgtt ggcgatggtg ttgaaggtcg gcaggatgcc
     2761 gttgcgcttg agcgggttgt aggtgtaggt gcccgagagt tgcagcgtat cgctgacgtt
     2821 gccgtcgagc cggaggaagc cgtagtcgcg gcggtcacgc gattcgtagg tgacgcccgt
     2881 gttgttcggg tagatgtagc tccggcgcgt cggcacgtcc tggatcgaat agctgctgaa
     2941 gaaccagagc cgatccttga tggccgggcc gctgaaggtg gccgacggga agaagcgcgt
     3001 gtactcgtcg cgcgggaagt ggaagtacgt gagcgtctgc tcgttggcgg actgcacggg
     3061 gcgcgtcccg gcggcgaggc ggctcacttc gaactgcgtg ccgatggagc cgtggtactc
     3121 gttgtcgccg cgcttggtgg cgacgttgat gacgccgccg gtggcgccgc cgaactcggc
     3181 ctcgaagccc gaggacttga cctgcacctc ctgcacgaac tcgaacggga tgttgttgtt
     3241 cacgttcagg gcgccggtgc ggaagttcgt cacctcctgg ccgtcgatga tgaaggtgtt
     3301 ctcggagccg gaggcgccgt cgatctggaa gccgcccgag agcggttcga cgcggacggc
     3361 gggcgacacc tgaaggacgg aggtgaagtt ggtgccgcgc ggggtcagct cgatcttctt
     3421 ctccgtgatg ttggtctgaa tccggctgtt gttggtgtcg atgggcgcgg catcagccgc
     3481 cgtgatgagc acctcttccg tgacctcgcc gacctccagc gcgacgttcg cgttggtggt
     3541 gttgccgagc acgacctcga cgcgctcctg cttcgcggtg cggaagctgg gggcgccggt
     3601 cgtgatcgtg tagaggccgg gcgggacttg caggacgcgg aaaaagccct cctcgtccgt
     3661 ggacgcggtg cggtcgaagg acgtgcccgt gatgcgcacg gtcgcccccg ccaccctggc
     3721 ccccgtgggg tcggatacgt agccttcgat gttgcccgtc gtctcctgcc ccagcacact
     3781 acctgccgag gccagcacga aggcggcaac cactaaacat agtctgatct tactcaccat
     3841 ctgtttcact ccttgctaag cgtgaaagaa ttcctccggc gttgcggcgg agagtacgtc
     3901 gtcgattacc tcactcgaac gccttgacgg ttgaagcgtt gtaactccga ggcgagagag
     3961 gcaattccaa agccgaggaa tgagatttga gtacaaacca gcggaaatta ctgctaaatt
     4021 ggcgaaaatc gcggcaaatg aatcgccccg cggcggacga cgggcgagct acggatgaag
     4081 aattagactt aatctaaccg gtcggacgac acgccgagtc ccccgatgaa actttggata
     4141 ccgtctacaa atttctgaaa aaaaataccg cgagcgggaa taaatacgga tgctcgtatc
     4201 gcgcctttca catcttgtcg ggcgctcgtc aggaggatga tgatgctgcg taagaacaga
     4261 ggtctctcgc gaagggttgt gctcggagtc gtgttggcgg gctggctggg cggcgtcggc
     4321 gcgcatccca cggacgctca cacatcggcg cgccgcgcgg cgcaggggag gaatgcgatc
     4381 agcggccacg tgttcgatac cgggcggcgt ccggtcgaag gcgtctacgt cgagctgctg
     4441 gacgaggtcg ataccgtcct caggaaggtg cggacggacg cgtccgggcg ctacgccttt
     4501 tccaacctgt cggacggcac ctttcaggtc agggtcttga cgcacgggac gaacctggtc
     4561 ggccagacgc ggcgggtctc gatcaccaac ttcatgggcg gcgctgccgg ccagtcctac
     4621 gaggaacatt tcacgctcgc ggcgagcaaa gcgccgggcg acacctcgac cgcggtcgcc
     4681 gcctcatccc ccggcctcgt cttcgttcag gagggcgtgc cggagaccgc gaaggcggct
     4741 tacgaaaggg gcgtcagaga gcttgaacgg gagggcggga acaaggcgtc ggcgctggcg
     4801 gagttgaaga aggcggtcga gctgttcccc acctattatc tggcgctgga gcaactgggg
     4861 gcggagtacg tcaggcagca gcagtacgag ccggcgcgcg ccgcgctgct caaggcggtg
     4921 gaggtcaacc cgcgcggcta caagagcctg tacgcgctcg gcgtggccca gtacaacctc
     4981 aagcagaagt ccgaggccac cgaatcgttg cgccgctcgg tcgagctgaa cccctcgtcg
     5041 gtcaactcgc agttgtggct gggcatcgtg ctcttcaagg acggcaaggc cgttcaggcg
     5101 gaaccccacc tcaagcgggc acacgaactc ggcgagaagc gcgtgcccga cgtacacatg
     5161 tatctggcac agctctacag cgacaccaag cgatacgcgc aggcggcgga cgagcttgaa
     5221 cactttctca aagaggcgcc cgcggcgcgc gacgcggaga acatcagaag gctcatcaag
     5281 caactgcgcg aaaaggcgag gtgagtttgg ccggaggccg accggtcgaa aaacggaagc
     5341 gctacttgag aagcagtccc ttacgcttac ggcgaggtgt ccccccacga cggcccccgc
     5401 aaggtcaata gcaagaatga cgccggcgaa gccggcagtg ccccgaggga gatgaagcgc
     5461 atgcggctgc tgaaactgag agtctgggac agaacacaga aaagattcca ctacggaggt
     5521 gcgtgccagg agttggggga gaatcaatgg ttcttcggac tgccgatccc gcacccggac
     5581 gactgcgagt tgatgcagtt caccggcgtc atcgacaaca acgggacgga gatttacgag
     5641 ggcgatgtcg tcgcctacta tcaaaacggc ctgcgcgaag tcagccagat catcttcagc
     5701 gacggcatgt tcaggatgga aaagtcatgc gtcgatctac agaactgggt ggatcgcaac
     5761 gaactcgaag tcatcggcaa catctactcc acgccggccc tctcccaaaa cggcgccgcc
     5821 gcggggtcgg aggccgacga gtcatgagga cgcccgcccg aacgtttcgc gcgtcgcgct
     5881 agacccgtcg ggcttgaacc ccagccggtc acaagaaacc ttatcggctc agagggcgcg
     5941 catcttccgc ggcgtcctcg cgtcggcatg agcatcgaag aaggggcggc ggacttcgtt
     6001 ggagagcgtc cgccgccctt ttttcctgac ttcgcgtttc ggtgtcgtct gtcgaaacgg
     6061 cccgagagcg cgggccgtct cccgcgccgg gaaactgcaa ggcgcggcct acctgctgcc
     6121 ggcagccgtc gagttgccga cgacgaacgc ctgcttctcg ccggcgaagg cgacgctgac
     6181 gagcgtgcgc gcgctgtcat ccgacagctt gacatccacc gtccttgaat tcgagctgcg
     6241 ctcgcccgtc gtggcggcga cgcgggccag cacctgcttc gacttcttgt cgctgacggt
     6301 cagctcgttg gtgccggtgt cgaagctgaa gatgtaggtg ccggccttga ccgccgtgcc
     6361 gccgacggtg aagtcctgtc cgatggtgat gtggcggctc ttgaccttcg ccagggacgg
     6421 ggcggcggcc aggacacaga ggagcagagc gagcgcgacg cgggtcatga ctttgtacat
     6481 ggttcgagat tcctttcgat gttttggagg cttgtttccg gcgcgacctt cgcagtccgg
     6541 aggtcgcgta cacacggcac atgtacgagg gcgggcggcg gtcgttcgac gaatgtgacg
     6601 aagcgacgct acggtgtgac ggactgacag gaaaggatga cgaagggagc gcgctgccgc
     6661 gggcggcgcg ccggccgcaa gggaatcacg ccacgatcat ggacgcgcgt ccggaagaat
     6721 gtccgaaccg acgaccgtgg aattgaatgc gatgcacttt catgttcggg taaagtgagg
     6781 ccgccgcgcg gaagactcgc acctcttcgc cgtgcccggc gcgccgcgac tctcgcgtcc
     6841 gggacatgcc ggaggtaatg tccgaagcgt cgggcgaccg tgtgcagaca cctgtggcgg
     6901 ccatgcgccg aggggcgtat actgcacgcg ccctagaaac tctcacggcc cgtctcaccc
     6961 tcgcgggagc gccctcacca tgccgaacat cctcctcatc gaagacatgc cgctcttgcg
     7021 cgacctgctc cgcgacgcgc tcgcgggtta cgtgtgccac gccgtcagaa cggcggagga
     7081 ggggctggag attctcgccc gcgtcccctt cgacgcggtg attacggacg tggggctgcc
     7141 gggcatgggc ggcgccgagc tgttgcggac gctgcgcgcg atacggcccc gcacgcccgt
     7201 catcgtcatc accggcggcg acgaggcgct gcgcgaagag gacttcatcg acctcggcgc
     7261 ttcggcctac ttgcaaaagc cgttcaccgt ggacgagttc atgcaggcgg tcgagcaggc
     7321 gctcgggcgg cgacgggcgt gagccgtccg gcgcgagagg ttaaactgtg gtcggacatg
     7381 ggcgaccgcg cggggtcgcg tgattaacca gccaacccgc gttacggagg gtacgacttt
     7441 ccatgaaacg attgatcttc aacgtcgcgg cgacgatgct ttcgactttg actttcgcgt
     7501 gcccggacgg tgacgcagag cctcacactc ccagcccgac ccccgctccc gggaaatacg
     7561 aaaaggcgat ccggaacctc gaagaaggta atgacaacca ggtcgtggag gaggcgatac
     7621 gggagttgta cgcggcgggg acggatgcct tccccgacct gtttgcacac ttcggcgatc
     7681 agcggaaagc ttccgtaaag catttcgggg cgaaggctct acagtgcgct ccgggggtag
     7741 aaccctgccc cccgtatcac cctacgattg gcgaagcttg cttcgatatc ctcggagggc
     7801 aagtcgaagg caattggccg aaaggggcta gaggttatta caccctcacg gcgggaaacg
     7861 ctaaggcgtg gtgggaagcc cggagaggga tgtcgctgaa ggaactccaa ctggaagccg
     7921 cccggtcttc gctcgcggcg gcgaggaagg atcgtgcggc gggcaaggcg agtaaggatt
     7981 tagtgaagtt cttggaagaa catctgaaag acgtgcgaga ggggcggtgc tgccctgaat
     8041 aagcagccgg ttccgtggcg aagatcggca tcagtcaacc gatgagaatc tacgaggact
     8101 acgcgcagtc gcccgacggg tggaacatca cgtctaccct gaagctggag gatgacggtc
     8161 ggttccaata tcaagagtca tggaccgact tcaccaacgc cactctcggg gtgacggctg
     8221 agggcagttg gcgacgggac gccgacgcca tcgttctcca cccgctccgt gtggaaggat
     8281 cggtaggcgg gtgggaatcg gcgaaggagc ggaaggccgt cgagcggggc gacattctgg
     8341 acttcggggg cggtctcaca ctccgcatcc cgccggagcg cgaacaagtc tttcccgtgc
     8401 gcaacaccgg cacgaagccg ctgaccgtcg tgcttgagcc gtggggcacg cgccacgtcg
     8461 tggcgccggg cgagcgtgcg cgtgtcgtcg cgcgcggccc cggcgggatg ggcgagcttg
     8521 agatcgtccg cggcgaggac gagatcgtgg tctacggctg gagcgaatcg cgggtcgccg
     8581 tcgttacgga gccgaagccc ctcggggcgg gcgccacgag tcgggcgccc ggcacaaaaa
     8641 agaccgtgca caccgtcaag gcgccggtcg cgccccttca gccggtgcgc gtcgccgagc
     8701 cggagatcgc acgggtcgag ccgctcgcgc cctcgcctga actcgccgcc cacatccggc
     8761 aatggatcga cgaactgccc acggagggac ttcggttccg gcttcagcgt gtctgtcagg
     8821 aactcgacgc cctgccgctc cacggcacgc tgatttacct ctgggcgctg cgacccgacg
     8881 gcgtcatcct gtgcatcgac cacgaagcgt tcggccaccc ggcggagcct gagagcgacc
     8941 cgctgaccgt ctacgccgtg ctcgctcaag gggcgcgcac gcaccccgaa ctcggcgaac
     9001 tgctcccgcc tcgccccgac ggtgtgcgcc ggtgcgaact ctgcgcgggc gcgggatgga
     9061 cgaaggccaa gccgcccgcg cagggttcat ccatctgctt ccgttgcaac ggcctggggt
     9121 ggtatgtcgc cggcacgccc cgctgacggc acgccccatc gaatgcgcat cggcgatcat
     9181 gtgatatgcc aatcggcgcc gtacgccgtc aggcccaact tcttcgccac cgcctgcgag
     9241 gcggtattct cccaggacgt gctgtacaag ggcacggcgc ccagagcgtg gaccagacgt
     9301 gcccacccgg ccaccacgtc tttcgcgtac cctctcccac gaaagtcggg cagcgtctcg
     9361 acgcccgcct cgtgcgccgc ggtggtgatg cgaacgctgc gacagacaga caccgcgcgg
     9421 ccctcctcga cgagggcgac gaagggttgc ccgtcgggca gttccgcgac cagctcctcg
     9481 aagcctccac gtaacaaccc ggcgtccgtt tccgtgacgg caagcagagg ccgcgacggc
     9541 tcgaagtatc gggtgaagtg ataggccggc cccatccacc gtctccgaac gggcgcgtgg
     9601 ccgtcgagga gccgcacgta tgcctcgaca tggcgaggct ccctttggaa ctcctgaccg
     9661 accggctcgt ccgcacacag cgcctccaat tcctcggcga gtgtctccgg aagatcggcc
     9721 cgcaatcgcc agaggttgcc ctcccgcgtc cggtccacga acagacgagg ggcgggaaca
     9781 tcgcctcccg gctcgttgac aaacaagagc cgcgagcggg cgtcgtgggt gaacaaggcc
     9841 cggacgtgca tggccatcag ctcttgatcc gtgagcacgt ctttcccctc tcacagctta
     9901 agtcgtccgg ctaacgcccg aatcaaccgg gcgggaagta acatcgaatg gcgtttaagt
     9961 tctcgatgag aggtatgctg attccgctcc ggttgaatgg gttataggtt atcggggttc
    10021 attcttcggc tctgattcat tagagttgga gcgagggaag aacgtgccgg gcgggcgtga
    10081 cgaatcttga atgaaaggcg
//
LOCUS       DBNGOH010001074         4034 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4928086, whole genome shotgun sequence.
ACCESSION   DBNGOH010001074 DBNGOH010000000
VERSION     DBNGOH010001074.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4034)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4034)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4034
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4928086"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..979)
                     /locus_tag="AC159P_20925"
     CDS             complement(<1..979)
                     /locus_tag="AC159P_20925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697966.1"
                     /translation="MGVLRKLLIVAAALVLAAVGWLWWNRVVKVDMAAYVPGDSLIYV
                     EANDLPRVLDGLTSTSAWRELAPAGRVRTDYERGVKLSGFAAATGIGPGDWVVLARAQ
                     LAVAVLGFDAAAEPDATLKFKPRLALVAETHTSEWRVRGVAEKLIGDFARRSFNAQDV
                     KRAETDGAHFLTWSDPNGSRRSLVAAVSGSVLVIGNDEAAVRSCLAVRRGERPSLAGN
                     EQLKLMRERLGAGESMAFGFAPAGSPAKIVEVFAPAFVPGFSPDQRMQSSMATFLPQL
                     ANRVVGDLGWSSKAADGAVEDRYFISLPEGMGGRLAGPFTPHPNREFGAG"
     regulatory      1384..1472
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            1630..1800
                     /locus_tag="AC159P_20930"
     CDS             1630..1800
                     /locus_tag="AC159P_20930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697967.1"
                     /translation="MAQASDTLKTKSAPPAAATAAPTHPAGRTLSPRVLRARARLRAL
                     HRRRFKESARNK"
     gene            1804..2877
                     /gene="pilM"
                     /locus_tag="AC159P_20935"
     CDS             1804..2877
                     /gene="pilM"
                     /locus_tag="AC159P_20935"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HIR3697968.1"
                     /translation="MFKRSLVKKSLVGLDIGSSSVKAVELQGKPGALSLASLGFAPLQ
                     PDTVVDGQIMELNDVSHVISAVFRSQQIKTERVAAGVSGSSVIVKNIIVPQMTREELE
                     ESIDWHAEEHIPFDISDVSLDYQVVGSGPDSLHVLMAACKRDFVANLKQAIQLAGKQP
                     AIIDVDAFALQNCYEVNYQPPDDLVVALLNIGASTMNINILKGTRSVFTRDVSVGGNQ
                     YTALLQKELGMTYEQAEMMKRGGGAAASAESAEEADVDGVLETVSDMLALEISKTFDF
                     YRATADDNEAAVQRILISGGGSKLKGLPEFLAQRFEIVVERLDPFRRISVDARRFDHD
                     YMSEVMPEMAVAVGLALRGVEAS"
     gene            2874..3566
                     /locus_tag="AC159P_20940"
     CDS             2874..3566
                     /locus_tag="AC159P_20940"
                     /inference="COORDINATES: protein motif:HMM:NF016992.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HIR3697969.1"
                     /translation="MIKVNLLESVTDRARAVAVVEERVTNPRARSLLLMVSVAGLMAL
                     GMGFDYVSANMAHRRAQAELERQQQIAAQMAAINKEQAELEKKIKDTQARIDAIKKLR
                     TSQQGPVALLSSLNERLPALPEFRLEAVEQKAGDLLIEGHSPNEDAVSQFGRSLEFSS
                     GLFTNVSIETEKKDMEVNVADYDLKNGPLDLSIKPETMRFKIKCRYTPAPAAAPQPPA
                     GAPPAANQVAQK"
     gene            3612..>4034
                     /locus_tag="AC159P_20945"
     CDS             3612..>4034
                     /locus_tag="AC159P_20945"
                     /inference="COORDINATES: protein motif:HMM:NF016253.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus inner membrane component PilO"
                     /protein_id="HIR3697970.1"
                     /translation="MSRLEALQEKPWYLQLAMFGGVAILLYVAFWYFVTSGTRAETAE
                     IETKVETMQRANAQAQIAAQRLNEFKALYQSTQANYEDLKALLPEQRELTMVLQGVQD
                     RARGRLSLRSFKPKEDVQQDFYSGKPVEVEVSGTYNNVG"
BASE COUNT          772 a         1382 c         1287 g          593 t
ORIGIN      
        1 ctcccgcgcc gaactcccgg ttcggatgcg gcgtgaaggg gccggccagc cgacctccca
       61 tgccttccgg cagcgagatg aagtagcggt cttcgactgc gccgtccgcg gccttcgaac
      121 tccaaccgag gtcgcccacg acgcggttgg ccagttgcgg gaggaaggtg gccatcgagc
      181 tttgcatccg ctggtcgggc gaaaagccgg ggacgaaggc cggcgcgaag acctccacga
      241 tcttggcggg gctgcccgcc ggcgcgaacc cgaaggccat cgactcgccc gcccccaaac
      301 gctcgcgcat cagcttcaac tgttcgttgc ccgcgaggct cggtcgctcg ccgcgtctca
      361 ccgcgaggca agagcgcacc gccgcctcgt cgttaccgat gacgaggacg ctacccgaca
      421 ccgccgcgac gagcgagcgg cgagagccgt tcgggtcgga ccacgtcaga aaatgtgcgc
      481 cgtccgtctc ggcccgcttc acgtcctgag cgttgaagga gcggcgcgcg aagtcgccga
      541 tcagcttctc ggccactccg cgcacgcgcc actcgctcgt gtgtgtctcc gcgacgaggg
      601 cgaggcgtgg cttgaatttc agcgtggcgt ccggctccgc cgccgcgtcg aagccgagca
      661 ccgccaccgc gagttgtgcg cgcgcgagca ccacccagtc gccggggccg atgccggtcg
      721 cggcggcaaa gccgctgagc ttgacgcccc tctcgtaatc cgtcctgaca cgtcccgcag
      781 gggcgagttc gcgccacgcc gaggtcgaag tcagcccgtc gaggacgcgc ggcaggtcgt
      841 tcgcctcgac gtagatcagc gagtcgccgg ggacgtaagc ggccatgtcc accttgacga
      901 cgcggttcca ccagagccag ccgacggcgg ccaggacgag cgccgccgcg acgatcaata
      961 acttccggag aactcccata tgactgaatc ctaacttccc gtcgccgcgc gaagcaagaa
     1021 ggtcttgaga tgcgcctcgt cgcgtcgggt gatccctgcg aggccgacgg acggggccgg
     1081 tgctttgaac tcgaatgccg aatcgcacgc gccatgaagc ccaaaagtat ttttgaaatc
     1141 aatccgaggg ctttctggaa tcgggccgga aaaatttcca gaaacaccta taagtgctga
     1201 aacgagatag gttaaactgt gcaaaatagt gattgacagt ggtcgatgca atgaattata
     1261 gtttctgtgt cgggcgcagt tcgcgctgcg gttccatgaa ccgtaaggcc cgcgaatgcg
     1321 aacgacgcgg cccgcccgca cagaggaaca cggctatccg gggcgcgccg tttcttcgaa
     1381 ctctaccgac aaaggcacac cacgcgcgag cgtgggtcgc aaagcctacg ggagtcagtc
     1441 atgcgcatga ctgacttggc cgggttgccg aagggagacc gcttgagccg acttcgcact
     1501 ccgcagtccg agcgcacttc gtttcacgca cccttccttc atccggagag agcttcacag
     1561 cccacacctt ctttcgcgcc gtccgcaaca ccctcccgcg ggcgcacgcc aaaggaaccg
     1621 actccgcaca tggcacaagc atccgacacg ctgaagacga agtccgcccc gcccgccgcc
     1681 gccaccgccg cgcccaccca ccccgccggg cgcacgctca gcccgcgagt cctgcgcgcc
     1741 cgcgcacgcc tccgggcgct ccaccgcaga aggttcaaag agtccgcaag gaacaaatag
     1801 tttatgttca agagaagtct cgtgaagaag agcctcgtcg gcctggacat cggctccagc
     1861 tcggtcaagg cggtcgagct tcagggcaag cccggcgcgc tctcgctggc gagcctgggg
     1921 ttcgcgccgc tccagccgga cacggtcgtg gacggccaga tcatggagct gaacgacgtg
     1981 tcgcacgtca tctcggcggt cttccggagc cagcagatca agaccgagcg cgtggcggcg
     2041 ggagtgtccg gcagctccgt catcgtcaag aacatcatcg tcccgcagat gacgcgcgag
     2101 gaactggaag agtccatcga ctggcacgcc gaggagcaca tcccgttcga catctcggac
     2161 gtgagcctcg actaccaggt ggtcggctcc gggccggact ctctgcacgt gctgatggcc
     2221 gcctgcaagc gcgacttcgt cgccaacctc aagcaggcga tccaactcgc gggcaagcag
     2281 cccgccatca tcgacgtgga cgccttcgcc ctccagaact gctacgaggt caactaccag
     2341 ccgcccgacg atctggtcgt ggcgctcctg aacatcggcg cctcgacgat gaacatcaac
     2401 atcctgaagg ggacgcggag cgtgttcacg cgcgacgtgt cggtcggcgg gaaccagtac
     2461 acggcgctct tgcagaagga gctgggcatg acgtacgagc aggccgagat gatgaagcgc
     2521 ggcggcggcg ccgcggcttc cgccgagtcc gcggaggagg cggacgtgga cggcgtgctc
     2581 gaaaccgtgt cggacatgct cgcgctcgaa atctcgaaga cgttcgactt ctaccgcgcg
     2641 accgccgacg acaacgaggc cgccgtgcag cggattctga tctccggcgg cggctcgaag
     2701 ctgaagggcc tgccggagtt cctcgcgcag cgcttcgaga tcgtggtcga gcgcctcgac
     2761 ccgttccgcc gcatcagcgt agacgcgcgg cgcttcgacc acgactacat gagtgaggtc
     2821 atgccggaga tggccgtcgc ggtcggtctc gctttgagag gagtggaggc gtcatgatca
     2881 aggtcaacct tctggaatcg gtcaccgacc gcgcgcgcgc cgtcgcggtc gtcgaggagc
     2941 gggtcaccaa cccgcgcgcg cgcagcctgc tgctgatggt cagcgtcgcc gggctgatgg
     3001 ccctcggcat gggcttcgac tacgtcagcg cgaacatggc gcaccgccgc gcgcaggccg
     3061 agctggagcg ccagcagcag atcgccgcgc agatggccgc catcaacaaa gagcaggccg
     3121 agctggagaa gaagatcaag gacacgcagg cccgcatcga cgccatcaag aagctgcgca
     3181 cctcgcagca ggggccggtc gcgctcctct cgtcgctgaa cgagcggctg cccgcgctcc
     3241 ccgagttccg cctcgaagcg gtcgagcaga aggccggcga cctcctgatc gagggccact
     3301 cgccgaacga ggacgccgtc agccagttcg ggcgcagcct cgaattctcc tcgggcctct
     3361 tcaccaacgt cagcatcgag accgagaaga aggacatgga ggtcaacgtg gccgactacg
     3421 acctgaagaa cggcccgctc gacctctcga tcaagcccga gacgatgcgc ttcaagatca
     3481 agtgccggta cacgccggcc cccgccgccg cgccgcagcc gcccgccggc gcgccgcccg
     3541 ccgccaacca ggtcgcgcag aagtaacgcg aggcgacggc gcccaccgcc gcccgaaggg
     3601 aagagaaacc gatgtccaga ctggaagccc tgcaagagaa accttggtat ctgcaactcg
     3661 ccatgttcgg cggcgtcgcc atcctgctgt acgtcgcctt ctggtacttc gtcacgagcg
     3721 gcacgcgcgc ggagaccgcc gagatcgaga cgaaggtcga gaccatgcag cgcgccaacg
     3781 cgcaggcgca gatcgccgcc cagcgcctca acgagttcaa ggcgctctac cagagcacgc
     3841 aggccaacta tgaagacctc aaggcgctgc tccccgagca gcgcgagctg acgatggtgc
     3901 ttcagggcgt gcaggatcgc gcgcggggcc gcctctcgct gcgcagcttc aagccgaagg
     3961 aggacgtgca gcaggacttc tactcgggca agcccgtcga ggtcgaggtc tccggcacct
     4021 acaacaacgt cggc
//
LOCUS       DBNGOH010001075         8722 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3279397, whole genome shotgun sequence.
ACCESSION   DBNGOH010001075 DBNGOH010000000
VERSION     DBNGOH010001075.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8722)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8722)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8722
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3279397"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..199)
                     /locus_tag="AC159P_20950"
     CDS             complement(<1..199)
                     /locus_tag="AC159P_20950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005416829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HIR3697971.1"
                     /translation="MREIDWFPLWLSLRVAFVSTVLVVVAGVSLAWALAAKSFRGREA
                     LDALVTLPLVLPPTVLGYYLLV"
     gene            365..1129
                     /gene="modA"
                     /locus_tag="AC159P_20955"
     CDS             365..1129
                     /gene="modA"
                     /locus_tag="AC159P_20955"
                     /inference="COORDINATES: protein motif:HMM:NF024921.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR3697972.1"
                     /translation="MKKVLLFILLAGFVAAGCAPRVEERPARDEIVVAAAANLTEAFE
                     EVGRRFTAQTGVRVVNSFGATADLAKQIEHGAPFDVFAAADVKHVEGLERKGLLADGS
                     RAVYARGRLVLWFSRGAQGARVEDLTGAGVTKVAVAKPELAPYGEAAVEALRSLGVWE
                     RVEPKVVYAQNVAQAKQFAATGNAEAAFVPRSLLKPNEGAAVEIDGALHKPIEQALGV
                     VKASKRQESARRFTDFVLSEEGQALLESYGYERGKR"
     gene            complement(1189..2226)
                     /locus_tag="AC159P_20960"
     CDS             complement(1189..2226)
                     /locus_tag="AC159P_20960"
                     /inference="COORDINATES: protein motif:HMM:NF012345.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HIR3697973.1"
                     /translation="MHEIKEHTTIDQPEPLPFPFNQRTVCRYERIEKRVARARVLVVN
                     DLLREESDLSDVARREWGARAAAHIERERYISGLALNNILGNITRLVREPETTVAHLS
                     EVTEAAREFRPDAIVLSGTLSDFDYYDPSMLERFGEFVRATTTPVLGICGGHQLVGLC
                     FGARVVTLDHREQHEQRVGRSFEYQYRFIRITAPEDPIFEGIHDEESGIWQDYTTEGR
                     ILRVWQNHGLQLDRVPEGFKLLATSYLCKNQMMVRREGGQLIHAVQFHLEKSFQDLHT
                     AASRARRINPNLPREERRRMLREEASSRTLWSHQNESRDGRILFENFLREAVRLKGEE
                     GKRGRGEEEEG"
     gene            complement(2355..2921)
                     /locus_tag="AC159P_20965"
     CDS             complement(2355..2921)
                     /locus_tag="AC159P_20965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697974.1"
                     /translation="MSAATAAQLKETRGASRALITARAPTADALAARVREELGEAWPG
                     AFYRERILPLRTRSHQVYAATKGAAVEVQPTLLGVELKGGRRRLSCPDLATARYLSVF
                     ARAGCTNVAVPYDITKISRLADELESAWQRMLLLAAHLSEGRGAAFQKRVTAAVVAEA
                     RRDIEEAGAGTPVPQFRQETSQRRRRIG"
     gene            complement(3123..4721)
                     /locus_tag="AC159P_20970"
     CDS             complement(3123..4721)
                     /locus_tag="AC159P_20970"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HIR3697975.1"
                     /translation="MIIFGQIAPAIPPDIDFRLLLPELILSVAGVLVMLVDAFARRTD
                     QRWLTGNLTLVGLLAAAGSCLWLWNPAPDVPQSAFGGMIVLDRLRLSFTFIFLLVAAL
                     TVLVSMIWVERERLPAGEFHTLLLFATVGMMLMASGGDLVMIFLGLEILSIATYVMCG
                     FRKNDLRSNESSLKYFILGSFSSAFLLYGIALVYGATAHTALVEGGRGVIAGTTNISL
                     IAERLNDGLYPPLLFAGAAMMLIGFGFKIATAPFHVWTPDVYEGAPTPVTAFMAAGPK
                     AAGFAAFMRVFLFAFPFVAVGQAIDTGARLHTAWLEALTIIAVLTMTVGNVVAIVQNN
                     VKRMIAYSSIAHAGYALVGFIAAGWAADAADRDNAVASVIFYLLVYSVMNLGAFAVVT
                     LMARGGDRRTDFDDYNGIGFRAPALSLTLSLFMLSLLGVPLTAGFMGKVMVFSEALDR
                     GLYWLVVIGVLNTAISTYYYLRLIVVMYFRERTSEWTPPYIPASVALVLVITVAGVLY
                     LGIFPGPVLDAFRAAQSIAVTHLH"
     gene            complement(4970..5536)
                     /locus_tag="AC159P_20975"
     CDS             complement(4970..5536)
                     /locus_tag="AC159P_20975"
                     /inference="COORDINATES: protein motif:HMM:NF012787.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HIR3697976.1"
                     /translation="MLLLSICCASLYAAAQQHRTPTPPAERKEVGFRLKALDGKTYDT
                     AATNGEVWVVSFGATWCTPCTWELVAIEELKEEYAGKPVRFLWISIEDEKRASNHILK
                     HYAKERRLTIPVLRDGDGSVLAQFTTTTRIPVVAFFGPDGKFDAPAHRGMSPDIADYK
                     QLVRRRVDALLKAAKGTTAAAGGAATAN"
     gene            complement(5603..7195)
                     /locus_tag="AC159P_20980"
     CDS             complement(5603..7195)
                     /locus_tag="AC159P_20980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045271 - respiratory chain complex I
                     [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048039 - ubiquinone binding [Evidence
                     IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="complex I subunit 4 family protein"
                     /protein_id="HIR3697977.1"
                     /translation="MQNYLLTILILLPAIGALAVALHGLAPYARPQHYRWVALGFSVA
                     TFLVSLLLLGGDVGGGSANAFRFEQKALWIGSVGAYYHLGVDGMSLWLVLLTTLLVPI
                     SILSSWHSIEKRGLAFFASMLVLEAAMIGVFVALDLLLFYLFFEASLIPMFILIGVWG
                     GERRLYAAIKFFIYTMVGSLLMLVGIIALYFMHAEAAGFGTFDYVTLFDAIQSGKLSL
                     VGEGGRATQEQLLLFLAFALAFSIKVPLFPFHTWLPDAHTEAPTAGSVILAGVLLKMG
                     TYGLMRFNFGLFPEATREMAWLFIILAIVGIIYGALVAMVQPDVKRLVAYSSVSHMGF
                     VVLGMFSFTEQGMQGALYQMLNHGVSTGALFLLVGMIYERRHTRMISEFGGLSKPMPW
                     FATFLVITSLSSVGLPLLNGFVGEFLIMLGMWSASPELTVGRPWVATMLAATGVIWAA
                     VYMLWMLQRVLFGRVTNPHNRRLPDLNAREIGLLLPLLALMLYMGAYPRPFLSRSRNS
                     VEQVRSRVVAPSSQGTFHADAR"
     gene            complement(7202..>8722)
                     /locus_tag="AC159P_20985"
     CDS             complement(7202..>8722)
                     /locus_tag="AC159P_20985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014785444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter transmembrane
                     domain-containing protein"
                     /protein_id="HIR3697978.1"
                     /translation="AMAVVAVIGAGTAIFAATIGIAQNDIKKVLAYSTVSQLGYMFLA
                     CGVGAFVAAIFHVMTHAFFKALLFLGSGSVIHGMHHEQDMRRMGGLKKYMPVTYWTMF
                     AGWLAICGIPIFAGFFSKDEILWKTFSTEVFGGPVGPILWGVGAVTALLTAIYMTRLM
                     AMTFWGEERFREAHAGGQADEAHAHAYEEKDKPHDAQLASEGDRTHHHPADSIDAHAH
                     EAAHAHSTAHDEAHGHDASHAHPSAHDADEDDEHEEHHHGPVEPHESPWSMTVPLVVL
                     AVLSTVGGLVGVPYALSSLFTAKEMNAFEHALDPVVHKAPTHAAAGAEHGPGAVPGEQ
                     AHGPELLTPPPQSHDGASPLSVGEGKAGAHGPATAHEAAAAHSPEHVKEERIFTVISV
                     VIALAGIGFGVWLFGRRPLLRMPRLLENKYYVDEVYDAALVNPIKEGSRGLLWRFFDV
                     GVIDGIVNGVGSVVTRFGAVARYLQPGFVRAYAAIILLGALAVVGFFAYNAYRALQ"
BASE COUNT         1562 a         2681 c         3135 g         1344 t
ORIGIN      
        1 gcacgagcag atagtagccg aggacggtcg gcggcaggac gagcggcagc gtgacgagcg
       61 cgtcgagggc ctcgcgcccg cggaaggact tcgcggcgag cgcccacgcg agcgagacgc
      121 ccgcgacgac gaccagcacc gtcgaaacga aagccacccg cagcgagagc cagaggggga
      181 accagtcaat ctctctcatc gactgcgaaa gatagcaccg gcgcggacga cgcggcaacg
      241 agcgcgccct ccgcgttgga gcctgaaatt aggatagact gagacgcgac ctgacttgca
      301 gtccgcgcgg tgtacaactt tcgtcgggag gtgttcgaga ttgaaagcaa tccgtgcgtc
      361 gttgatgaag aaggtgcttc tgttcatcct gctcgcgggt ttcgtggcgg cgggttgcgc
      421 gccgcgggtc gaggagcggc ccgcgcggga cgagatcgtc gtcgccgccg cggcgaacct
      481 gacggaggcg ttcgaggagg tcggcaggcg gttcacggcg cagaccggcg tgcgtgtcgt
      541 caacagcttc ggggcgacgg cagaccttgc gaagcagatc gagcacggcg cgccgttcga
      601 cgtgttcgcg gcggcggacg tgaagcacgt cgaaggtctg gaaagaaagg ggctgctggc
      661 ggacggctcg cgggcggtgt acgcgcgcgg gcggctcgtg ctgtggtttt cgcgcggcgc
      721 gcagggcgcg cgcgtcgaag acctcacggg cgcgggcgtc acgaaggtcg ccgtggcgaa
      781 gccggagctg gcgccgtacg gcgaggcggc ggtcgaggcg ctgcggtcgc tcggcgtgtg
      841 ggagcgcgtc gagccgaagg tggtctacgc gcagaacgtg gcgcaggcga aacagttcgc
      901 ggcgaccggc aacgccgagg cggcgttcgt cccgcgctca ctgctgaagc cgaacgaggg
      961 cgcggcggtc gagatcgacg gcgcactcca taagccgatc gaacaggcgc tcggcgtcgt
     1021 caaggcttcg aagaggcagg aatcggcgcg caggttcacg gacttcgtcc tgagcgaaga
     1081 agggcaggcg ctgctcgaaa gctacggcta cgagagaggg aaaagataag ggggaagagg
     1141 ggaagagggg aaaaggggaa gaggaagagg gttgagcgtg atgctgtttc aaccttcttc
     1201 ctcttcccct cttcccctct tcccctcttc ccccttaagt cttaccgctt cgcggaggaa
     1261 gttttcgaag aggatgcgac cgtcgcgcga ttcgttctgg tgcgaccaga gggtgcggct
     1321 gctggcctct tcgcggagca ttcggcgacg ctcttcgcgg ggcaggttgg ggttgatgcg
     1381 acgcgcgcgg ctggcggcag tgtgcaggtc ctggaaagat ttttcgaggt ggaactggac
     1441 ggcgtggatc agttggccgc cctcgcgccg caccatcatc tggttcttgc agaggtagga
     1501 ggtggcgagc agcttgaagc cttcgggcac gcggtcgagt tgcaggccgt ggttctgcca
     1561 gacgcgcaga atccgcccct cggtcgtgta gtcctgccag atgcccgact cctcgtcgtg
     1621 gatgccctcg aagatggggt cttcgggcgc ggtgatgcgg atgaagcggt actggtattc
     1681 gaacgagcga ccgacgcgct gctcgtgctg ttcgcggtgg tcgagcgtga cgacgcgcgc
     1741 gccgaagcag aggccgacga gctggtggcc gccgcagatg ccgagcacgg gagtcgtcgt
     1801 ggcgcggacg aactcgccga agcgttcgag catcgagggg tcgtagtagt cgaagtcgct
     1861 gagcgtgccg ctgaggacga tggcgtcggg gcggaactcg cgcgcggcct cggtcacttc
     1921 ggagaggtgc gcgaccgtcg tctccggctc gcggacgagg cgcgtgatgt tgccgaggat
     1981 gttgttgagg gcgaggccgg agatgtagcg ctcgcgttcg atgtgcgccg cggcgcgcgc
     2041 gccccactcg cggcgtgcca cgtcggagag gtcggactct tcgcgcagca ggtcgttgac
     2101 gacgagcacg cgcgcgcgcg cgacgcgctt ctcgatgcgc tcgtagcggc agaccgtgcg
     2161 ctggttgaag gggaagggta acggctccgg ctggtcgatg gtggtgtgtt ctttgatctc
     2221 gtgcatcatt gatggtgaat agtaaatggt aaatggtaaa tagaaaaacc gtctctgtgc
     2281 gtatccgttg acgctgacat caactcgaaa gaagttctct atttaccatt cgccattttc
     2341 catttactag cttactaacc gatgcgccgg cggcgctggc tcgtctcctg tctgaattgc
     2401 gggacaggcg tgccggcccc ggcttcttcg atgtcgcgtc gggcctcggc gacgaccgcc
     2461 gccgtgacgc gcttctggaa cgccgccccg cgcccttcgg agaggtgcgc ggcgagcagg
     2521 agcatccgct gccaggccga ttcgagttcg tccgcgaggc gcgagatttt ggtgatgtcg
     2581 taggggacgg ccacgttggt gcagcccgcg cgggcgaaga ccgagaggta gcgcgcggtg
     2641 gcgaggtcgg ggcaggagag gcggcgtcgc ccccccttca actcgacgcc gagcagcgtg
     2701 ggctggactt cgaccgccgc gcccttcgtc gccgcgtaga cctgatgcga gcgcgtgcgt
     2761 aaggggagga tgcgctcgcg gtaaaaggcg cccggccacg cttcacccag ctcctcgcgg
     2821 acgcgcgcgg cgagcgcgtc ggcggtcggc gcgcgcgccg tgatgagcgc ccgcgaagcg
     2881 ccccgcgtct ccttcagttg tgccgccgtg gccgcgctca tccgccttcg cacctataaa
     2941 taaaatcggc ggcgaaaaca gtctcgcccc cgtcaccact atagattaac acaccaccgg
     3001 cgacgagggc gaaggggaaa acgtcaggga aaaggtcaaa aggggaagag gcgaaaaggg
     3061 atgacaagcg acttgcttgc cccttttccc cttttcgcct tttgaccttt ccgcctcttc
     3121 ccttaatgca gatgcgtgac ggcgatggac tgcgcggcgc ggaaggcgtc gaggacgggg
     3181 ccggggaaga tgccgaggta gagcacgccc gcgaccgtga tgacaaggac gagggcgacg
     3241 ctggccggga tgtagggcgg cgtccactcg gaggtgcgct cgcggaagta catgacgacg
     3301 atgaggcgca ggtagtagta ggtcgagatg gccgtgttga ggacgccgat gacgacgagc
     3361 cagtagaggc cgcggtcgag cgcctcgctg aagaccatca ccttgcccat gaagccggcg
     3421 gtgagcggga cgcccagcag gctcagcatg aagagcgaga gcgtcaaaga gagcgcgggc
     3481 gcgcggaagc cgatgccgtt gtagtcgtcg aagtcggtgc ggcggtcgcc gccgcgcgcc
     3541 attagcgtga cgacggcgaa ggcgccgagg ttcatcaccg agtagaccag caggtagaag
     3601 atgaccgagg cgacggcgtt gtcgcggtcg gcggcgtccg ccgcccaccc ggcggcgatg
     3661 aagccgacca gcgcgtagcc cgcgtgcgcg atggaagagt aggcgatcat ccgcttgacg
     3721 ttgttctgca cgatggcgac gacgttgccc acggtcatcg tgaggacggc gatgatcgtc
     3781 agcgcttcga gccaggccgt gtgcaggcgc gcgccggtgt cgatggcctg cccgacggcg
     3841 acgaagggga aggcgaagag gaagacgcgc atgaaggcgg cgaagcccgc cgccttcggc
     3901 ccggcggcca tgaaggccgt gacgggcgtc ggcgcgccct cgtacacgtc gggtgtccag
     3961 acgtggaagg gcgcggtcgc gatcttgaag ccgaagccga tgagcatcat cgccgcgccc
     4021 gcgaacagca gcggcgggta gaggccgtcg ttcagccgct cggcgatgag gctgatgttg
     4081 gtcgtcccgg cgatgacgcc gcgcccgcct tcgacgagcg cagtgtgcgc cgtcgcgccg
     4141 tagacgagcg cgatgccgta gagcaggaag gccgacgaga acgagccgag gatgaagtac
     4201 ttgagcgaag actcgttcga gcgcaggtcg ttcttgcgga agccgcacat cacgtaggtc
     4261 gcgatggaga ggatttcgag gccgaggaag atcatcacca gatcgccgcc cgacgccatc
     4321 agcatcatcc ccacggtggc gaacagcagc agcgtgtgga actccccggc gggcaggcgc
     4381 tcgcgctcga cccagatcat cgagaccagc acggtgagcg cggcgacgag caggaagatg
     4441 aaggtgaagg agagccgcag gcggtcgagc acgatcatgc cgccgaaggc gctctggggt
     4501 acgtccggcg cggggttcca cagccagagg caggagccgg cggcggccag cagcccgacg
     4561 agcgtgaggt tgcccgtcag ccagcgctga tccgtgcgcc gcgcgaacgc gtccaccagc
     4621 atgacgagca cgcccgcgac cgagaggatc agctccggca ggagcagccg gaagtcgatg
     4681 tcgggcggga tcgccggggc aatttggccg aagataatca tcgtcactcg aataaagctc
     4741 cacagaaggc gcgtcagtcc gccgcggagc ggcgactgag tttagcccgg cctttcaagg
     4801 ccgggaaccg gcgcaccatt cgtcgccagc gtcgcgtagc gacgcgtgaa tcgtgcgtgt
     4861 ccgttcaggc gtcgctgacg cgacgcgaat cgcccccggc gcggctgacc cggcgttgaa
     4921 acgccgggct acattcatct cgccgctacg cggcggggcg gactgaatgt caattcgccg
     4981 tcgccgcgcc gcccgccgcc gccgtcgtgc ccttcgcggc cttgagcagc gcgtccacgc
     5041 ggcggcgcac caactgtttg taatcggcga tgtccggcga catgccgcgg tgcgcgggcg
     5101 cgtcgaactt gccgtcgggg ccgaagaagg cgacgacggg gatgcgcgtc gtggtcgtga
     5161 actgcgcgag taccgagccg tcaccgtcgc gcagcacggg gatcgtcaac cgccgctctt
     5221 tggcgtagtg cttgaggatg tggttcgagg cgcgcttctc gtcctcgatg ctgatccaga
     5281 ggaagcggac gggctttccg gcgtactcct ccttcaactc ctcgatggcc accagctccc
     5341 acgtgcaggg cgtgcaccac gtcgcgccga aggagacgac ccacacctcg ccgttcgtcg
     5401 ccgcggtgtc gtacgtcttg ccgtcgagcg ccttcagcct gaagccgact tccttgcgct
     5461 cggcgggcgg cgtcggcgtc cgatgctgct gcgccgccgc gtacagagag gcgcagcaga
     5521 tggataacag gagcaaggcc gtcaatgtga atcgtctaaa catggtgcat gacaagaacg
     5581 cttcttgaac tctgttcaat cttcaacgcg cgtcggcgtg gaacgtgccc tgcgacgagg
     5641 gcgcgacgac gcgcgagcgc acctgctcga cgctgttgcg cgagcggctg aggaacgggc
     5701 gcgggtaagc gcccatgtag agcatcaacg cgagcagcgg caggagcaag ccgatctccc
     5761 tcgcgttcaa gtccggcagc cggcggttat gcgggttggt cacgcgcccg aagagcacgc
     5821 gctgcaacat ccacagcatg tacacggcgg cccagatgac gcccgtcgcg gcgagcatcg
     5881 tcgccaccca cgggcgcccg acggtcagct cgggactcgc cgaccacatg ccgagcatga
     5941 tgaggaactc gccgacgaag ccgttcagta gcggcaggcc cacggacgag agcgacgtga
     6001 tgacgaggaa cgtggcgaac cacggcatcg gtttggacag cccgccgaac tccgagatca
     6061 tacgcgtgtg gcgccgctcg tagatcatgc cgaccaagag gaagagcgcg cccgtcgaga
     6121 cgccgtggtt gagcatctgg tagagcgcgc cctgcatgcc ctgctccgtg aacgagaaca
     6181 tgccgaggac gacgaagccc atgtggctca ccgaagagta ggcgaccagc cttttcacgt
     6241 cgggctgcac catcgccacc agcgcgccgt agatgatgcc gacgatggcg aggatgatga
     6301 agagccacgc catctcgcgc gtcgcctcgg ggaacagccc gaagttgaaa cgcatcagcc
     6361 cgtaggttcc catcttcagc agcacccccg cgaggatgac gctccccgcc gtgggcgcct
     6421 ctgtgtgcgc gtcgggcagc cacgtgtgga aggggaagag aggcaccttg atcgagaagg
     6481 cgagggcgaa ggcgaggaag agtagtaact gctcctgcgt cgcgcgcccg ccctcgccga
     6541 ccaggcttag cttgccgctc tgaatggcgt cgaagagcgt gacgtagtcg aaggtaccga
     6601 agcccgcggc ctctgcgtgc atgaagtaga gcgcgatgat gccgaccagc atcaggaggc
     6661 tgccgaccat cgtgtagatg aagaacttga tcgccgcgta gagcctgcgc tcgccgcccc
     6721 agacgccgat gaggatgaac atcgggatga gcgacgcctc gaagaagagg tagaagagca
     6781 gcaggtcgag cgcgacgaag acgccgatca tcgccgcttc cagcacgagc atcgaggcga
     6841 agaaggcgag gccgcgcttc tctatcgaat gccagctcga caggatcgag atggggacga
     6901 gcagcgtcgt cagcagcacg agccagaggc tcatgccgtc cacgccgagg tggtaatagg
     6961 cgccgacgct cccgatccag agcgccttct gctcgaagcg gaacgcgttc gcggaacctc
     7021 cgcccacgtc gccgcccagc aggagcagcg agacgagaaa ggtggcgacc gagaagccga
     7081 gcgcgaccca gcggtagtgc tgcgggcgcg cgtacggggc gagtccgtgc agcgcgaccg
     7141 cgagggcgcc gatggccggg agcaggatca ggatcgtgag caggtagttc tgcatgacgt
     7201 tttactgtag ggcgcggtaa gcgttgtagg cgaagaaccc gacgacggcc agcgcgccga
     7261 gcaggatgat ggccgcgtag gcgcggacga agcccggctg caaatagcgc gcgacggcgc
     7321 cgaaccgcgt caccacgctg ccgacgccgt tcacgatgcc gtcgatcacg cccacgtcga
     7381 agaaccgcca cagcagcccg cgcgagcctt ccttgatcgg gttgacgagc gccgcgtcgt
     7441 agacctcgtc cacgtagtat ttgttctcca gcaggcgcgg catccgtaag agcggcctgc
     7501 gcccgaagag ccacacgccg aagccgatgc ctgcgagcgc gatgacgacc gaaatgaccg
     7561 tgaagatgcg ctcttctttg acgtgctcgg ggctgtgcgc cgccgccgct tcgtgggccg
     7621 tggccgggcc gtgcgcgccg gccttgccct cgccgaccga gagcggcgag gcgccgtcgt
     7681 gcgactgcgg cgggggcgtc agcagctccg gcccgtgcgc ctgctcgccg ggcaccgcgc
     7741 cggggccgtg ctcggcgccc gccgcggcgt gcgtcggcgc tttgtggacg accgggtcga
     7801 gcgcgtgctc gaaggcgttc atctccttcg ccgtgaacag agagcttagc gcgtagggca
     7861 cgccgaccag tccgccgacg gtggagagca cggcgagcac gaccagcggc accgtcatcg
     7921 accacggcga ctcgtgcggc tcgacggggc cgtggtggtg ctcttcgtgc tcgtcatctt
     7981 cgtccgcgtc gtgcgccgac gggtgagcat gcgaggcgtc gtgaccgtgc gcttcgtcgt
     8041 gcgccgtcga atgagcgtgc gcggcttcgt gcgcgtgcgc gtcgatgctg tcggcggggt
     8101 gatgatgcgt tcggtcgccc tcggaggcga gttgcgcgtc gtgcggcttg tccttctcct
     8161 cgtaggcgtg agcgtgcgcc tcgtcggcct gcccgcccgc gtgggcctcg cggaaacgtt
     8221 cctcgcccca gaaggtcatc gccatcagcc gcgtcatgta gatggccgtc aggagtgcgg
     8281 tcacggcgcc gacgccccac aggatcggcc ccacagggcc gccgaagact tcggtgctga
     8341 aggtcttcca cagaatctcg tccttcgaga agaagcccgc gaagatgggg atgccgcaga
     8401 tggcgagcca gcccgcgaac atcgtccagt aggtgacggg catgtatttt ttgaggccgc
     8461 ccatccgccg catgtcctgc tcgtgatgca tgccgtggat gacggagccg gagccgagga
     8521 agagcagcgc cttgaagaag gcgtgcgtca tgacgtggaa gatcgccgcg acgaaggcgc
     8581 cgacgccgca cgcgaggaac atgtagccga gctgcgagac ggtcgagtag gcgaggactt
     8641 tcttgatgtc gttctgcgcg atgccgatgg tcgcggcgaa tatcgcggtc cccgcgccga
     8701 tcaccgccac aacggccatc gc
//
LOCUS       DBNGOH010001076        10681 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2912408, whole genome shotgun sequence.
ACCESSION   DBNGOH010001076 DBNGOH010000000
VERSION     DBNGOH010001076.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 10681)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10681)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10681
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2912408"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            140..1348
                     /locus_tag="AC159P_20990"
     CDS             140..1348
                     /locus_tag="AC159P_20990"
                     /inference="COORDINATES: protein motif:HMM:NF024959.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-alanine-activating enzyme beta-propeller
                     domain-containing protein"
                     /protein_id="HIR3697979.1"
                     /translation="MKEATAKEEMTKRETTNEAERAGASPFSRRSFLLLPLALYLSPG
                     TARARSWNNYAAPADAWPQFRGNPQLTGFSRSGVPDRMRVLWTFEAGESVESSAAIAG
                     GVAYVGTQKGELLAVGLGDGQARWRYAAKAEIGESSPAVGAGLVFVGDLSGVVHGVTA
                     SDGRGAWTFKTGAEVKSSPVVAGNRVLVGSYDGHLYCLAAATGKLLWKVKTGAAVHAT
                     AGVAEGTAFITGCDEILRAVRITDGRQLYQVRSGAYTGASPALLAGNAFYGTFNNDVL
                     GVNLVARQVGWRYRNPERQFPFYSSAAIIDARVVVGGRDKAVHCLDAYTGNAHWTFQT
                     QARVDSSPALAGNRVFVGSNDGRLYMLDFYSGRKVAEFNVGAPVSASPAVASGRVVVG
                     SQDGKLYCFG"
     gene            complement(1436..1960)
                     /locus_tag="AC159P_20995"
     CDS             complement(1436..1960)
                     /locus_tag="AC159P_20995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697980.1"
                     /translation="MRKIFTAAALALAALALVAVSAAAQTGQAGQKASFAGTWVLDQG
                     RSYNLPRTVKQTMTVVQDGDKLTVETEIESEQGKRTVKDVYTLDGKEADFTPQIQNDP
                     NAKGKRTGRWVPGGKSFVVEETIATKNPEGAPVQITITRKWLLWADGSLSIDIHQDTP
                     RGSSESRRFFTKKT"
     gene            complement(1994..2965)
                     /locus_tag="AC159P_21000"
     CDS             complement(1994..2965)
                     /locus_tag="AC159P_21000"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HIR3697981.1"
                     /translation="MEEIPPDETPLATEAGDRLAARLAAGFFGGRRARLRRIVGKGSV
                     NQVFVAEGGDGERIVVRMCDGPYALEQHEKEAWCIAQARGVGVPGPEVFAVGVADGAA
                     YMIESFVEGAVGDELPAAAAPRVWRKLGQYARAIHTIETDGFGVNLPDFTSGDARGGW
                     LRYVDYNVESLDERDELLRLGVLAWEQSALVRAIFEELRSREFRFGVNHGDLSLKNTI
                     VDDAGRVHLLDWGTAFADIVPHHDLVQLLKKQLTEGEPDDAGLAAFLEGYGMAREEYE
                     ALLPELESVLLLRSFDLVRYAIDRCPDHIPFYAARARLALARKFAHS"
     gene            complement(2990..4618)
                     /locus_tag="AC159P_21005"
     CDS             complement(2990..4618)
                     /locus_tag="AC159P_21005"
                     /inference="COORDINATES: protein motif:HMM:NF012686.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HIR3697982.1"
                     /translation="MIPAIVVFIYLAAVVYIGVFAFRKSKGTHGAEDYFLANRSLGTF
                     VFLMSLFGTNMTAFAILGSSGHAFNNGIVTFGLMASSSGLVIPLTIFLCGTRVWALGK
                     KYGFMTPVQMFRDRWECGHIGTVIFAVQAVLLVPYIIIGIMGGGTTLNAISGGRVPFW
                     FGGAIVAMVVMSYVFFGGMRGTAWVNTFQTILFLVFGAIAVTVIGTGMGGFSAAVEAL
                     EKSPAFAPLLTRERISPYYFFSYTFIPLSAIAFPHIAIFCLTAKKMSQFKKTVILYPL
                     CIMAIWLPCVFLGVMANRVTDVPQIRAKQEARRVLATQGKTMEPEQREELREKSAGDD
                     VILLLLERFAPLWLAGLLGAGIMAAVMASDSQILALSTMFTEDVFAFYGGKARFGESV
                     QVQTGRIFVIILTILAYAVALRAPESIFELAIQYAFSGFAALSPLLIAALFWRGSTKW
                     GALAATVWTISAVLAVAVFQEVVPAPAPGTTTVVATLGGFEFLARTYGGTTVFGFMPV
                     VPMVIVSALLMLVVSLLTSKPSRTTVEKYFPARA"
     gene            complement(4615..4866)
                     /locus_tag="AC159P_21010"
     CDS             complement(4615..4866)
                     /locus_tag="AC159P_21010"
                     /inference="COORDINATES: protein motif:HMM:NF023183.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3311 domain-containing protein"
                     /protein_id="HIR3697983.1"
                     /translation="MKKLLLVLAVATLYVLHQDVWNWRVARVNGYMPLGAVPLGLFYH
                     ACFSVAASLVMWLLVKFAWPSHLEQEVEGQASADERSAR"
     gene            complement(5201..6454)
                     /locus_tag="AC159P_21015"
     CDS             complement(5201..6454)
                     /locus_tag="AC159P_21015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HIR3697984.1"
                     /translation="MPHFPAAATGATGEATGVLVTLFAVIVAAKLFAELFERLRQPAV
                     AGEILAGVVVGPSVLGLVTPTEMTNVLAEMGVIFLLFNVGLETKPAALLRVGKTATVV
                     AVLGVIAPFVCGWLLMRAWGGNNVEALFLGTAMVATSVGITARVLSAMGKLDTETARI
                     ILGAAVIDDILGLIILAVVSGTAGGTLDLVEIGTTAALAVGFTLFVAFVGAPVVTRAA
                     PRVEALRFSHSLFAFGLVLCLGLSAAASYIGVAVIIGAFLAGMALAEGAENQPTMHAQ
                     TNGVTELLVPFVLVNIGMQLKLDVFRDPAVVLLSIVVTLVAVATKFFACGAAARGLGR
                     RRAAQVGMGMVPRGEVGVVVAQIGLALAVIGPELYGVVLFMAVATTLVAPPFIKLLFA
                     SEPEEAHDSPAGGAAVVIHEPDRIG"
     gene            6558..8012
                     /locus_tag="AC159P_21020"
     CDS             6558..8012
                     /locus_tag="AC159P_21020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011557038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR3697985.1"
                     /translation="MSELTKTINTEPEEKAAPEPGFFEAVWQAVRGTHVTYDYTEGDI
                     GRSIMLLAVPMVLEMLMESVFAVVDVFFVARLGPEAVAAVALTESMLTLIYTLAIGLG
                     IGATAMVARRIGEHDAEGASRAAVQTIALGLLISVVLGVTGVVLGPRLLAVMGASPEV
                     IERGSGFTRVMLGGSVTVVMLFLINAVFRGAGDAAIAMRVLWLANAINIVLCPCFIFG
                     LGPFPELGVTGAAVATTIGRGTGALYAFSRLLRPGARVRLTRHNLKLEPSVMMRLVRL
                     SGTATFQVFIGMASWIGLVRILANFGDVALAGNTIGMRIILFALLPSWGMSNAAATMV
                     GQALGAGKPERAEKAVWRAGFYNMIFLGVVGLLFVLFADQIVSVFTQAAAGPEVQRYG
                     AECLRIVACGFLFYAYGMVITQSFNGAGDTWTPTVINLFVFWLWEIPLAYVLAFHLGL
                     GPRGVFLAVTIAFSTLAVVSAYVFRKGRWKQKVV"
     gene            8045..8365
                     /locus_tag="AC159P_21025"
     CDS             8045..8365
                     /locus_tag="AC159P_21025"
                     /inference="COORDINATES: protein motif:HMM:NF020908.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ family domain-containing protein"
                     /protein_id="HIR3697986.1"
                     /translation="MNFDKLVEQKIKEAMEAGEFDRLEGEGRPVDLDAYFSAPEELRA
                     GFAVMKNANVVPEEMQLRGEINQLKRELEACADAARQEALKKDIAELMLKYDLMVERN
                     RKGK"
     gene            8877..>10681
                     /locus_tag="AC159P_21030"
     CDS             8877..>10681
                     /locus_tag="AC159P_21030"
                     /inference="COORDINATES: protein motif:HMM:NF018190.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate binding family 9 domain-containing
                     protein"
                     /protein_id="HIR3697987.1"
                     /translation="MKTGSRPPAPLSLVLVLLCLTVGAHAQSASYAAAGNTATPTPTA
                     TPAPAETPASVASTSSTPATTAAAAAATNNAAADGGAAPAPATKKVGNVMLPPEKTQP
                     TRIPKFDAVPVIDGKLDEAVWKQAAVLKDFYQVDPGDNSAPSKQTEVLIGYDAKTLYI
                     AFKANDEPDKVRATTPRRDQIFQDDYVGFFLDTFNDQRRAYCIFFSPTGVQADGIMTE
                     GRGEDYSVDIVMDSKGIVTSDGFVVEIAIPFKSLRYEAGKGKNWGAHFFRRIKRISNE
                     LDSWMPFSRDVTGNLNQAGKLTGLEGIATERTIEVIPSLTLDQTGRRYRTIPIWEVSD
                     LRRRGLNPVDPSRLVNEPVSLDPGLTAKFGISPQLTLDLALNPDFAQVEADQLVVTAN
                     QRFPIFFAERRPFFLEGKEIFETRIQAVHTRAIVDPDVAVKLTGKQGKNTFGVMYASD
                     NAAGNLSIDEREIAADTRFDATQPGRRQSLLNILDQNATFGVLRVKRDVGRQSHLGFI
                     GTANKFVDERYNYLGGIDGRFKWAKNWLAEFQVLGTSSLNNFFDPAANSTRKRVGNAF
                     AYSGEIEYSGRNLYFELSTSGRTRYYRADVGFFQRT"
BASE COUNT         1879 a         3593 c         3554 g         1655 t
ORIGIN      
        1 gcgggccagt ggacgggccg cttcaacccg cgaccgttcg accgcgcggg cgctttagag
       61 gtttacgagt gcgcgtactg acgcaagggc gaaaggcaaa ggggaagggc gaagggaaga
      121 ggagttgaag gaagcagtga tgaaggaagc gacggcgaaa gaagagatga cgaagcgaga
      181 gacgacgaat gaagcggagc gggccggcgc gtctcctttc tcgaggcgct ctttcctgct
      241 gctgcctctc gcgctttacc tgtcgccggg gacggcgcgg gcgcgctcgt ggaacaacta
      301 cgcggcgccc gccgacgcgt ggccgcagtt ccggggcaac ccgcagttga ccggcttctc
      361 gcgctcgggc gtgcccgaca ggatgcgcgt gctgtggacg ttcgaggcgg gcgagtcggt
      421 cgaatcctcg gcggccatcg cgggcggcgt cgcttacgtc gggacgcaga agggcgagct
      481 gctggccgtc ggtctgggcg acggccaggc gcgctggcgc tacgcggcga aagccgagat
      541 cggcgagtct tccccggcgg tcggggcggg gctggtcttc gtcggcgacc tctcgggcgt
      601 ggtgcacggc gtcactgcga gcgacggtcg cggcgcgtgg acgttcaaga cgggcgccga
      661 ggtgaagtcg tcgcccgtcg tcgcgggcaa ccgcgtgctc gtcggctctt acgacggcca
      721 tctctactgc ctcgcggcgg cgaccggcaa gcttttgtgg aaggtgaaga cgggggccgc
      781 cgtgcacgcg accgcaggcg tagccgaggg cactgctttc atcacgggct gcgacgaaat
      841 cctgcgggcg gtgcgtatca cggacgggcg gcagctttat caggtgcggt cgggcgcgta
      901 cacgggcgcg tcgcccgcgc tgctggcggg caacgccttc tacggcacct tcaacaacga
      961 cgtgctcggc gtcaacctcg tcgcgcggca ggtcggttgg cgctaccgca acccggagcg
     1021 gcagttcccg ttctactcct cggcggcgat catcgacgcg cgcgtggtcg tcggcgggcg
     1081 cgacaaggcc gtccactgtc tcgacgccta cacgggcaac gcgcactgga cgttccagac
     1141 gcaggcgcgc gtcgattcct cgcccgccct cgcgggcaac cgcgtcttcg tcggctcgaa
     1201 cgacgggcgc ctctacatgc tcgatttcta cagcgggcgc aaggtcgccg agttcaacgt
     1261 cggcgcgccg gtctcggcct cgcccgccgt cgcctcgggc cgcgtcgtcg tcggctcgca
     1321 ggacggcaag ctctactgct tcggataaag aagtgctgcg tactgagtgc tgcgtactga
     1381 gtagaagcag tcgtctttta ctcagtactc agcactcagt acgcagcact tctttttatg
     1441 ttttcttcgt gaagaagcgg cgcgactcgg aggagccgcg gggcgtgtcc tggtggatgt
     1501 cgatggagag cgagccgtcg gcccagagca gccacttgcg ggtgatggtg atctggacgg
     1561 gcgcgccctc ggggttcttc gtggcgatgg tctcctcgac gacgaaggac ttgccgcccg
     1621 gcacccagcg gcccgtgcgc ttgcccttcg cgttggggtc gttctgaatc tgcggcgtga
     1681 agtccgcctc cttgccgtcg agcgtgtaca cgtccttcac ggtgcgcttg ccctgctcgg
     1741 actcgatctc ggtctcgacc gtcagcttgt cgccgtcctg cacgaccgtc atcgtctgct
     1801 tcacagtgcg cggcaggttg tagctgcggc cctgatcgag cacccacgtg ccggcgaagc
     1861 tcgccttctg ccccgcctgc cccgtctgcg ctgccgcgct gacggcgacc agcgcgagcg
     1921 ccgcgagggc gagcgccgcg gcggtgaaaa tcttcctcat cttccgttcc tccgtaatcg
     1981 acgtttcgag cgttcaagag tgcgcgaact tgcgcgcgag cgcgagtcgt gcgcgcgccg
     2041 cgtagaaagg gatgtgatcc gggcacctgt caatcgcgta gcgcacgagg tcgaaggagc
     2101 gcagcagcag gacgctctca agctcgggca gcaacgcttc gtactcctcg cgcgccatcc
     2161 cgtagccttc gaggaacgcg gccagccccg cgtcgtccgg ctcgccctcc gtcaactgtt
     2221 tcttcaacaa ctgcacgagg tcgtggtgcg gcacgatgtc ggcgaaagcc gtcccccagt
     2281 cgagcaggtg gacgcgcccc gcgtcgtcaa caatcgtgtt cttcaaagaa aggtcgccgt
     2341 gattcacacc gaagcggaac tcgcgcgacc gcagctcctc gaagatcgcg cgcacgagcg
     2401 ccgactgctc ccacgcgagc acgccgaggc gcagcagctc gtcccgctcg tcgagactct
     2461 cgacgttgta atcgacgtac cgcagccagc cgccgcgcgc gtcgccgctc gtgaagtcgg
     2521 gcaggttgac gccgaagccg tcggtctcga tcgtgtgaat cgcccgcgcg tactggccga
     2581 gcttgcgcca gacgcgcggc gcggctgccg ccggaagctc gtcgcccacc gcgccctcga
     2641 cgaacgactc gatcatgtag gccgcgccgt cggccacgcc gaccgcgaaa acttccggcc
     2701 ccggcacgcc gaccccgcgc gcctgcgcga tgcaccaagc ctccttctcg tgctgctcca
     2761 gcgcgtaagg cccgtcgcac atccgcacga cgattctttc gccgtcgccg ccttcggcca
     2821 cgaagacctg attgacggaa cccttgccga cgatgcgtcg cagcctcgca cgccgcccgc
     2881 cgaaaaaacc cgccgcgagc cgcgccgcca gccggtcgcc cgcttcggtc gccagcggcg
     2941 tctcgtccgg cggtatctct tccatcgcgg tctcggcggg tcggcgagtt caggcgcgcg
     3001 cggggaagta tttctccacg gtggtgcggc tcggcttcga cgtgagcagc gagacgacaa
     3061 gcatcaggag cgccgagacg atgaccatcg ggacgacggg catgaagccg aagacggtgg
     3121 tgccgccgta ggtgcgcgcg aggaattcga agccgccgag ggtggcgacg accgtcgtcg
     3181 tgccgggcgc gggcgcgggg acgacctcct gaaaaacggc gacggcgagg acggcggaga
     3241 tcgtccagac cgtggcggcg agcgcgcccc acttggtgct gccgcgccag aagagggcgg
     3301 cgatcagaag gggcgaaaga gccgcgaagc cggagaaggc gtactggatg gcgagttcga
     3361 aaatcgactc gggcgcgcgc agcgcgacgg cgtaggcgag gatcgtcagg atgatgacga
     3421 agatgcgccc ggtctgcacc tgcacggatt cgccgaagcg ggccttgccg ccgtagaagg
     3481 cgaagacatc ctccgtgaac atggtcgaga gcgcgagaat ctgcgagtcc gaggccatga
     3541 cggcggccat tatccctgcg ccgagcagcc ccgcgagcca gagcggggcg aagcgttcga
     3601 gcagcagcag aatcacgtcg tcgcccgccg acttctcgcg cagctcttcg cgttgttccg
     3661 gctccatcgt cttgccctgc gtggcgagca cgcgccgcgc ctcctgcttg gcgcgaatct
     3721 gcggcacgtc cgtgacgcgg ttcgccatca cgccgaggaa gacgcaaggg agccagatcg
     3781 ccatgatgca gagcgggtag aggatgacgg tcttcttgaa ctgcgacatc ttcttggccg
     3841 tcaggcagaa gatggcgatg tgcgggaagg cgatggccga gagcgggatg aaggtgtagc
     3901 tgaagaagta gtaaggcgag atgcgttcgc gcgtcaggag cggcgcgaag gcgggcgact
     3961 tttccaacgc ctcgaccgcg gcggagaagc cgcccatccc cgtgccgata accgtcaccg
     4021 cgatggcgcc gaagaccaga aacagaatcg tctggaacgt gttgacccac gccgtgccgc
     4081 gcatgccgcc gaagaagacg taagacatga cgaccatcgc cacgatggcg ccgccgaacc
     4141 agaagggcac gcgcccgccg ctgatggcgt tcagcgtcgt gccgccgccc atgatgccga
     4201 tgatgatgta ggggacgagc agcacggcct gcacggcgaa gatgacggtg ccgatgtggc
     4261 cgcactccca gcggtcgcgg aacatctgca cgggcgtcat gaagccgtac tttttaccca
     4321 aagcccagac gcgcgtcccg cacaaaaaga tcgtgagcgg gatgacgagg cccgaagacg
     4381 aggccatcag gccgaaggtg acgatgccgt tgttgaaggc gtggcccgac gagccgagga
     4441 tggcgaaggc cgtcatgttc gtcccgaaca gcgacatcag gaagacgaac gtgccgagcg
     4501 agcggttggc gaggaagtag tcctccgcgc cgtgcgtgcc cttcgacttg cggaaggcga
     4561 agacgccgat gtagacgacc gcggcgaggt agatgaagac gacgatggcc ggaatcaacg
     4621 cgcgctcctt tcgtctgcgg acgcctgccc ttcgacctcc tgctcaaggt gcgagggcca
     4681 cgcgaacttg accagcagcc acatcacgag cgacgccgcc accgagaagc aggcgtggta
     4741 gaagaggccg agggggactg cgccgagcgg catgtaaccg ttcacgcggg cgacgcgcca
     4801 gttccaaacg tcctggtgca gcacgtagag ggtggcgacg gcgagcacga gcaggagctt
     4861 tttcataagc cgttcattat gtccaatccg gcggacggac agtgtaaaaa ggttgtaaaa
     4921 attggccgat gcgtgcggag actcaaagcg tcaagcgccc cattgtacgc gcgaggccgc
     4981 cgagttgttt agaacaattt gggggttgag ctgaccgcgg gcaggattca acgcggagcg
     5041 cgcagagaaa cgcagagcgc gcggagagga caaaaaaacg gattcggaat tcgaaatctc
     5101 gaaatcgctc tttcttctct gcactctctc tgcgcccctc ctgtgtcctc tgcgttgaag
     5161 cctgcccgcg gtcagctcaa cggcccgttc ttcttcggcc ttagccgatg cggtcgggtt
     5221 cgtggatgac gaccgccgcg cctccggcgg gactgtcgtg cgcctcctcc ggctcggagg
     5281 cgaagagcag cttgatgaag ggcggcgcga cgagcgtcgt ggcgacggcc atgaagagca
     5341 cgacgccgta cagctccggg ccgatgaccg cgagcgccag accgatctgc gcgacgacga
     5401 cgccgacctc gccgcgcggc accattccca tcccgacctg cgcggcgcgc cgccgcccca
     5461 gaccccgcgc cgccgcgccg cacgcgaaga acttcgtggc gacggcgacg agcgtcacga
     5521 cgatcgacag caggacgacc gcggggtcgc ggaacacgtc gagcttgagc tgcatcccga
     5581 tgttgacgag gacgaagggg acgagcagct ccgtcacgcc gttggtctgc gcgtgcatcg
     5641 tcggctggtt ctcggcgcct tcggcgagcg ccatcccggc gaggaaggcg ccgatgatca
     5701 ccgccacccc gatgtagctg gccgccgccg agaggcccag acacaggacg aggccgaagg
     5761 cgaagagcga gtggctgaag cgcagcgcct cgacgcgcgg cgcggcgcgc gtgaccacgg
     5821 gcgcgccgac gaaggcgacg aagagcgtga agccgacggc gagcgcggcg gtcgtgccaa
     5881 tctcgaccag atcgagcgtg ccgcccgccg tgcccgagac gacggcgagg atgatgagac
     5941 cgaggatgtc gtcgatcacc gccgcgccga gaatgatgcg cgccgtctcg gtgtcgagct
     6001 tgcccatcgc ggagagcacg cgcgccgtga tgccgaccga cgtggcgacc atcgccgtgc
     6061 cgaggaacag cgcctcgacg ttgttcccgc cccacgcgcg catgagcagc cagccgcaga
     6121 cgaacggcgc gatgacgccg agcaccgcga ccaccgtcgc cgtcttgccg acgcgcagca
     6181 gcgccgcggg cttcgtctcc agcccgacgt tgaagagcag gaagatgacg cccatctcgg
     6241 cgaggacgtt cgtcatctcc gtgggcgtga caaggccgag cacgctcggc ccgacgacga
     6301 cgccggccag aatctcgccc gccaccgcgg gctggcgcag ccgctcgaag agttccgcga
     6361 acagcttggc cgcgacgatg acggcgaaca gcgtcacgag cacgcccgtc gcttctccgg
     6421 tagcgcccgt ggccgcggct gggaaatgtg gcatcgactc tcccgttcaa aacaaatcgc
     6481 gcgccggtat cgtagtatag cgcttaatca acctcggcgg ctcccggctc cagaaaccgc
     6541 agttggcggg aggaatgatg tccgaactga ccaagactat caacacggaa ccggaagaaa
     6601 aagccgcccc cgagccgggg ttcttcgaag ccgtctggca ggccgtgcgc ggcacgcacg
     6661 ttacttacga ctacaccgag ggcgacatcg gtcgctcgat catgctcctg gccgtcccga
     6721 tggtgttgga gatgctgatg gagagcgtct tcgccgtcgt ggacgtgttc ttcgtggcgc
     6781 ggctcggccc cgaggcggtg gcggcggtcg cgctcaccga gtcgatgctg acgctcatct
     6841 acacgctggc catcggcctc ggcatcggcg ccaccgcgat ggtcgcgcgc cgcatcggcg
     6901 agcacgacgc ggagggcgcc tcgcgcgccg ccgtgcagac catcgcgctc ggcctgctca
     6961 tctcggtcgt gctcggcgtg acgggcgtcg tgctcgggcc gcggctgctc gccgtgatgg
     7021 gcgcctcgcc cgaggtcatc gagcgcggga gcgggttcac gcgcgtgatg ctcggcggca
     7081 gcgtcacggt cgtgatgctc ttcctcatca acgccgtctt ccgcggcgcg ggcgacgcgg
     7141 ccatcgcgat gcgcgtgctg tggctggcga acgccatcaa catcgtgctc tgcccgtgct
     7201 tcatcttcgg cctcgggccg ttccccgaac tcggcgtgac gggcgccgcc gtggcgacca
     7261 ccatcgggcg cggcacgggc gcgctctacg ccttctcgcg gctgctgcga ccgggcgcgc
     7321 gcgtgcgcct gacgcggcac aacttgaaac tcgaaccatc ggtgatgatg cggctggtgc
     7381 gcctgtcggg gacggcgacg ttccaggtct tcatcgggat ggcgagctgg atcgggctgg
     7441 tgcgtatcct cgccaacttc ggcgacgtgg cgctcgcggg gaacaccatc gggatgcgga
     7501 tcatcctgtt cgcgctgctg ccctcgtggg ggatgtcgaa cgcggcggcg acgatggtcg
     7561 ggcaggcgct cggcgcaggg aaacctgagc gcgccgagaa ggccgtctgg cgcgctgggt
     7621 tctacaacat gatcttcctc ggcgtcgtcg gcctgctgtt cgtgctcttc gccgaccaga
     7681 tcgtctcggt cttcacgcag gccgccgccg ggccggaggt gcagcgctac ggcgccgagt
     7741 gcctgcgcat cgtggcgtgc gggttcctgt tctacgccta cggcatggtc atcacgcagt
     7801 cgttcaacgg cgcgggcgac acgtggacgc cgaccgtcat caacctcttc gtcttctggc
     7861 tgtgggagat tccgctcgcc tacgtcctcg ccttccatct gggcctgggg ccgcgcggcg
     7921 tcttcctcgc cgtcaccatc gccttctcga cgctcgccgt cgtcagcgcc tacgtcttcc
     7981 gcaaggggcg ctggaaacag aaggtggtct gaatcgacct cgcgcgcgcg ggaggtaaag
     8041 ctctatgaac ttcgacaagc tggtcgagca gaagatcaaa gaggcgatgg aggcgggcga
     8101 gttcgaccgc ctcgaaggcg aggggcggcc cgtcgatctg gacgcctact tctcggcgcc
     8161 cgaggagctg cgcgcgggct tcgccgtgat gaagaacgcc aacgtggtgc ccgaggagat
     8221 gcaattgcgc ggcgagatca accaactgaa gcgggaactc gaagcctgcg ccgacgccgc
     8281 gcgccaagag gctttgaaga aggatatcgc cgagttgatg ctgaagtacg acctcatggt
     8341 cgagcgcaat cggaaaggaa agtgagcctg tgggcacgcc gggcgcgtct cctcctgcgt
     8401 gcacgtcgga gcggtcggcg attgagttta ccgggaggcc cgtttcaaca cagaggggac
     8461 ggaggacaca cagagaccac ggagaagaca atcgggtttc tctgtgtcct ccgtgaatcc
     8521 tccgtgccct ctgtgttgaa atgaaccttc ggtaaactca cccgcgaacc ttaccggaac
     8581 cccgaccgca accgaatcgt atcgaagacg cgccctccga aaagtccgcg agggttcagc
     8641 cctccccacg cccttcagcc ggacgcgccc cgcaaatttt gccacgaact cgacgcgcgc
     8701 ggtgtttcgc aggtagagca aggcaagaac gctctcgccg cagaccgcta gctcgtccgc
     8761 cgagaaaaac tgaaacctta cggacgggcc ggacactaac tggccgagtc gcagccggca
     8821 ccggcgcgcg tccgaaaaac accctcgaat tatttacgac agattggact cctaacatga
     8881 agacaggttc acggccaccc gcgccgctct cgctggtact cgtcctcctc tgcctcacgg
     8941 tcggcgcgca cgcgcagtcc gcgtcctacg ccgcggcggg caacacggcc acgccgaccc
     9001 ctacggcgac gcccgccccc gccgaaacgc ccgccagcgt ggcgagcacg tcgtcaacac
     9061 cggcgacgac cgccgccgcc gccgccgcga cgaacaacgc cgccgcggac gggggcgcgg
     9121 cgcccgcgcc cgcgacgaag aaggtcggca acgtcatgct gccgcccgag aagacgcagc
     9181 ccacgcgcat cccgaagttc gacgccgtgc ccgtcatcga cggcaagctg gacgaggccg
     9241 tctggaagca ggccgccgtc ctcaaggatt tctatcaggt cgatcccggc gacaactccg
     9301 ccccgtccaa gcagaccgag gtcttgatcg gctacgacgc caaaacgctc tacatcgcct
     9361 tcaaagccaa cgacgagccg gacaaggtgc gcgccaccac gccgcgccgc gaccagattt
     9421 ttcaggacga ctacgtcggc ttcttcctcg acaccttcaa cgaccagcgc cgcgcctact
     9481 gcatcttctt cagcccgacg ggcgtgcagg ccgacggcat catgaccgag ggccgcggcg
     9541 aggactacag cgtcgatatc gtgatggact cgaagggcat cgtcacctcg gacggcttcg
     9601 tcgtcgagat cgccatcccc ttcaagtcgc tgcgctacga ggccgggaag ggcaagaact
     9661 ggggcgcgca cttcttccgc cgcatcaagc gcatctcgaa cgagctggac tcgtggatgc
     9721 ccttctcgcg cgacgtgacg ggcaacctca accaggcggg caagctgacg ggcctcgaag
     9781 gcatcgccac cgagcgcacc atcgaagtca tcccgagcct gacgctcgac cagacgggcc
     9841 gccgctaccg caccatcccc atctgggagg tgtccgacct gcgccgacgg ggcttgaacc
     9901 cggttgaccc gagccgcctc gtcaacgaac ccgtcagcct cgaccccggc ctgacggcca
     9961 agttcggaat ctcgccgcag ctcactttgg acctcgcgct caaccccgac ttcgcgcagg
    10021 tcgaagcaga ccagctcgtc gttaccgcca accagcggtt ccccatcttc ttcgccgagc
    10081 ggcgcccctt cttcctcgaa ggcaaggaga ttttcgagac gcggatacag gccgtccaca
    10141 cgcgcgccat cgtcgatccg gacgtggccg tgaagctcac cggcaagcag ggcaagaaca
    10201 ccttcggcgt gatgtacgcc tcggacaatg ccgccggcaa cctcagcatc gacgagcgcg
    10261 agatcgccgc cgacacgcgc ttcgacgcca cgcagccggg ccgccgccag tcgctcctca
    10321 acatcctcga ccagaacgcc acgttcggcg tcctgcgcgt caagcgcgac gtgggccggc
    10381 agtcgcacct cggcttcatc ggcacggcca acaagttcgt ggacgagcgc tacaactacc
    10441 tcggcggcat cgacgggcgc ttcaagtggg ccaagaactg gctcgcggag tttcaggtgc
    10501 tcggcaccag ctcgctcaac aacttcttcg acccggcggc caactcgacc cgcaagcgcg
    10561 tcggcaacgc cttcgcctac tcgggcgaga tcgagtactc gggccgcaac ctctacttcg
    10621 agctgagcac gtcgggccgc acccgctact accgcgccga cgtgggcttc ttccagcgca
    10681 c
//
LOCUS       DBNGOH010001077         3623 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4378864, whole genome shotgun sequence.
ACCESSION   DBNGOH010001077 DBNGOH010000000
VERSION     DBNGOH010001077.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3623)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3623)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3623
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4378864"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..683
                     /locus_tag="AC159P_21035"
     CDS             <1..683
                     /locus_tag="AC159P_21035"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024760.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HIR3697988.1"
                     /translation="AMVGLGFAMTENIKYYGEAVLAGNAAGVFVVRGLFSPFAHPLFT
                     SMTGIGLGLARQSRSRAVKLTAPVIGLALAVLLHGAWNGTIYLGSRMESGVLILLTYF
                     VVMVPIFAGLLTAIVLALRHEGRVLREHLWRDCERGVFSRDEYLRLCSLRARAGASFD
                     ALTRGGVAQWRARRQLNGLASELAFHRSRVARGITARDGSDAQREAGYVRQIYALCAQ
                     LGGPRAAR"
     gene            781..1557
                     /locus_tag="AC159P_21040"
     CDS             781..1557
                     /locus_tag="AC159P_21040"
                     /inference="COORDINATES: protein motif:HMM:NF024118.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3697989.1"
                     /translation="MKIKLLPSTFDARGLATPEQRLSCFLVDGRVAIDAGSIALALTD
                     AERETVRDIIVTHPHMDHIATLPIFIDDLFATLEEPVRVHGTEEVIGMLARDVFNGTV
                     YPPFQDFSNERTRVMEFVPFRTGEEFRVAHLTLTAVPVHHIVPTVGMVFTDGAATIAF
                     SSDTASTEDFWELVNAAPRVDSLLIEASFPNSLSKLAEVSGHLTPAALGAELGKLKHD
                     GLDILAVHLKPSFREQLIEELAALGIPHLSAMEPGREYSW"
     gene            complement(1695..>3623)
                     /locus_tag="AC159P_21045"
     CDS             complement(1695..>3623)
                     /locus_tag="AC159P_21045"
                     /inference="COORDINATES: protein motif:HMM:NF015140.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007154 - cell communication [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Calx-beta domain-containing protein"
                     /protein_id="HIR3697990.1"
                     /translation="GNYSLTLTSVGGGCASSPTVPGGGAHNGTLAAPDCRFVDDSFFD
                     PFSFVATAGQRISITYTPTFDGIVYLLDSTRTIIAVDGGLGGSFNARIPASGLVTLPT
                     SGTYFVVAGSVPRNDPGSPTPTGSYSLTIEDGGQCPTAPITTGQTINNSLNLGECVLP
                     DRTLVDFYTFNGTAGQEVVISLSSTAFDTFLFLNGPAGTVVAEDDDGGGGLDSRIPAG
                     SGTFTLPATGTYTILANTFPPGPGQPSPTGNYTLSLTGTVPPAADVQFAATALSATEG
                     TDRSVLVNVTRTGVTTGESFVSFATADGTTNERRDYTIALGRLRFAAGETNKSFEVVI
                     TDDAFAEGPEAFSITLSNPVGAALGAQSTATVTITSNDATNGPSPVRDASFNTEFFVR
                     QHYADFLNRVPDAGGLQFWIDNIESCGANQQCREVRKVDTSAAFFLSIENQETGFLAH
                     RTHKAAFGNLAGKPVPVTLRSFLSDSRQIGDGVIVGTPGWPEKLEANKVAYFNEFVTR
                     AAFIAANPLSLTAAEYVDGLFARAGVTPTAAERQAAINAFGAGGTAGRAAALRSVAES
                     ATLRTAETNRSFVLMQYFGYLRRNPDDPDFQNNPDPNFLGYNFWLTKLNQFNGNFVDA
                     EMVKAFIQSIEYGDRFGQ"
BASE COUNT          543 a         1230 c         1276 g          574 t
ORIGIN      
        1 cggcgatggt cggcctcggc ttcgccatga ccgagaacat caagtactac ggcgaggccg
       61 tgctcgcagg caacgccgcg ggcgtcttcg tcgtgcgcgg gctgttctcg cccttcgcgc
      121 acccgctctt cacgagcatg acgggcatcg ggctggggct ggcgcgccag tcgcgcagcc
      181 gcgccgtcaa actcaccgcg cccgtcatcg ggctggcgct ggcggtgctg ctgcacggcg
      241 cgtggaacgg cacgatctac ctcggcagcc ggatggagag cggcgtgctc atcctgctca
      301 cctacttcgt cgtgatggtg ccgatcttcg ccgggctgct cacggccatc gtcctcgcgc
      361 tccgccacga ggggcgcgtg ctgcgcgagc acctgtggcg cgactgcgag cgcggcgtgt
      421 tctcgcgcga cgagtacctg cggctgtgca gcctgcgcgc gcgcgcgggc gcctccttcg
      481 acgcactgac gcgcggcggc gtggcccagt ggcgcgcccg ccgccaactg aacgggctgg
      541 cgagcgagtt ggccttccac cgcagccgcg tcgcgcgcgg catcacggcc cgcgacggat
      601 cggacgcgca gcgcgaggcc gggtacgtgc ggcagattta cgcgctgtgc gcgcagctcg
      661 gcggcccgcg cgccgcgcgc tagagccgtc atccgccgac ggccccgccg acgtactttc
      721 cggcgagacc gctttttctt tacaaccctc gaagaggaca gccagaccgg aacctgacaa
      781 gtgaagatca aactcctgcc aagcacgttc gacgcccgcg gtctggcaac gccggagcag
      841 cggctgagct gcttcctcgt tgacgggcgc gtggcgattg acgccgggag catcgcgctc
      901 gccctcaccg acgccgagcg cgagacggtg cgcgacatca tcgtcacgca cccgcacatg
      961 gatcacatcg ccacgctccc gatcttcatc gacgacctct tcgccacgct cgaagagccg
     1021 gtgcgcgtcc acggcacgga ggaggtcatc gggatgctcg cgcgcgacgt gttcaacggc
     1081 accgtctacc cgccgttcca agacttcagc aacgagcgca cgcgcgtcat ggagttcgtc
     1141 ccgttccgca cgggcgagga gttccgcgtc gcgcacctca cgctcaccgc cgtccccgtc
     1201 caccacatcg tcccgaccgt cggcatggtc ttcaccgacg gcgcggcgac catcgccttc
     1261 agctccgaca ccgcctcgac ggaagacttc tgggagctgg tcaacgccgc gccgcgcgtg
     1321 gactcgctgc tcatcgaagc ctcgttcccg aactcgctct cgaagctcgc cgaggtctcc
     1381 ggccacctca cgcccgcggc gctcggcgcc gaactcggca agctcaagca cgacggcctc
     1441 gacatcctcg ccgtccacct caagccctcc ttccgcgaac agctcatcga agaactcgcc
     1501 gcgctcggca tcccgcacct ctcggcgatg gagccgggcc gcgagtactc atggtgacaa
     1561 aagcgagccg gtagagagcc gcgcctacga aaacagcccg acgataaaga gcgggtcgat
     1621 agaaaaaaag tccggcggcg ctcgggtgtc gagcgccgcc ggagtcgttc gaaggagccg
     1681 ctgtcggcag ccggttactg gccgaagcgg tcgccgtact cgatggactg gatgaacgcc
     1741 ttgaccatct cggcgtccac gaagttgccg ttgaactggt tgagcttcgt gagccagaag
     1801 ttgtagccga ggaagttcgg gtcggggttg ttctggaagt ccgggtcgtc cgggttgcgc
     1861 ctgagatacc cgaagtattg catcagcacg aacgatctgt tcgtctccgc cgtcctcagc
     1921 gtcgccgact cggcgaccga acgcagcgcc gccgcgcgtc ccgccgtgcc gcccgcgccg
     1981 aaggcgttga tcgcggcctg acgttccgcc gccgtgggcg tgacgcccgc ccgcgcgaac
     2041 agcccatcca cgtactcggc ggcggtcagc gagagggggt tggcggcgat gaaggcggca
     2101 cgcgtgacga actcgttgaa gtaggcgacc ttgttcgctt cgagcttctc gggccagccc
     2161 ggcgtgccga cgatgacgcc gtcgccgatc tgccgcgagt ccgagaggaa gctgcgcagc
     2221 gtcaccggca cgggcttgcc cgccagattg ccgaacgcgg ccttgtgcgt gcggtgggcg
     2281 aggaagccgg tctcctggtt ctcgatcgag aggaagaagg cggcgctcgt gtccaccttc
     2341 cgcacctcgc ggcactgctg gttggcgccg cacgactcga tgttgtcgat ccagaactgg
     2401 aggccgcccg cgtccggcac gcggttgagg aagtccgcgt agtgctggcg gacgaagaac
     2461 tcggtgttga acgaggcgtc cctgaccggg ctggggccgt tggtcgcgtc gttgctggtg
     2521 atggtgacgg tggcggtgct ctgcgcgccg agcgcggcgc cgaccgggtt gctcaaggtg
     2581 atgctgaagg cctccgggcc ttcggcgaag gcgtcgtcgg tgatgaccac ctcgaagctc
     2641 ttgttggtct cgcccgcggc gaagcgcagc cgacccagcg cgatggtgta gtcgcgccgc
     2701 tcgttcgtgg tgccgtcggc ggtggcgaag ctgacgaagg actcgccggt ggtgacgccg
     2761 gtgcgcgtga cgttgacgag gacgctgcgg tctgtgccct cggtcgccga cagcgccgtg
     2821 gcggcgaact gcacgtccgc ggcgggcggg acggttcccg tcaggctcag cgtgtagttg
     2881 ccggtcggag acggctggcc cggccccggc gggaacgtgt tggcgagaat cgtgtacgtg
     2941 ccggtcgccg gcagcgtgaa cgtcccgctc ccggcgggga tgcgcgagtc gaggccgccg
     3001 ccgccgtcgt cgtcctcggc cacgacggtg cccgccggcc cgttcaggaa gaggaacgtg
     3061 tcgaaggccg tcgaagagag cgagatcacg acctcctgcc cggcggtgcc gttgaaggtg
     3121 tagaaatcga cgagcgtcct gtcgggcagg acgcactcgc cgaggttcag cgagttgttg
     3181 atcgtctgcc cggtcgtgat cggcgcggtc gggcactggc cgccgtcctc gatcgtcagc
     3241 gagtagctgc cggtcggcgt cggcgagccg gggtcgttgc gcgggacgct gccggcgacg
     3301 acgaagtagg tgcccgaggt gggcagcgtg acgaggccgc tcgccgggat gcgcgcgttg
     3361 aaactgccgc ccagcccgcc gtccacggcg atgatcgtgc gcgtcgagtc gagcaggtag
     3421 acgatgccgt cgaaggtcgg cgtgtaggtg atcgaaatcc gctgcccggc ggtggcgacg
     3481 aagctgaacg ggtcgaagaa gctgtcgtca acgaagcggc aatcgggcgc ggcgagcgtg
     3541 ccgttgtgcg cgccgccgcc cggcacggtg ggcgaggagg cgcacccgcc gcccacgctc
     3601 gtcagcgtca gcgagtagtt gcc
//
LOCUS       DBNGOH010001078         5486 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3463115, whole genome shotgun sequence.
ACCESSION   DBNGOH010001078 DBNGOH010000000
VERSION     DBNGOH010001078.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5486)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5486)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5486
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3463115"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..537
                     /locus_tag="AC159P_21050"
     CDS             <1..537
                     /locus_tag="AC159P_21050"
                     /inference="COORDINATES: protein motif:HMM:NF012234.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR3697991.1"
                     /translation="NRSFFLNELDGFASNTGVVVVATTNHPERLDPAILDRPSRFDRK
                     FYFELPAAEVRLLYARRWNELLQPETRLGEGALAETAARTEGFSFAYLKELFVASLME
                     WMNAGGARSMGEVLAERTARLIEQMSSRDDDGGKDAGEEGGSKGEDEDEESDDDETDD
                     DADHMTRVNVRARGAGQG"
     gene            complement(534..1733)
                     /gene="nagA"
                     /locus_tag="AC159P_21055"
     CDS             complement(534..1733)
                     /gene="nagA"
                     /locus_tag="AC159P_21055"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HIR3697992.1"
                     /translation="MTNNEATPERLLLRHARLVLPARIAEDASLLVEGGRIARVLEGD
                     ESAGATHLQTIELAGTTLYPGFVDVHIHGAVGVDTMEASADDLHAVARFLASRGVTAW
                     LPTFVPAPEEDYARAARAIEELMRTQDALPAAARAVGVHYEGPFVNERQCGALRVPFF
                     RTYDSAADVEALPRVNAEGAAHMITVAPEVAGGVELIGELRRRGWVVSVGHTRADIDM
                     LERARAAGARHMTHFPNAMPPLHHRAPGPVAWGLLSEDVTVDVIADGVHSDPVMVELV
                     LRTKTPARVALISDAVAPAGLGDGEYRVWGETITVAGGRTSNERGAIAGSVITMLDAA
                     RLVSRLGFAETDLARMAALNPARLLGLDAVCGSVEEGKRADLVALDAEGRPRLTLVGG
                     RVAHEAL"
     gene            complement(1869..3197)
                     /locus_tag="AC159P_21060"
     CDS             complement(1869..3197)
                     /locus_tag="AC159P_21060"
                     /inference="COORDINATES: protein motif:HMM:NF051698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HIR3697993.1"
                     /translation="MSPFGESVFEVSKRDFAPRVGLAWDPFGKGRTAVRTGYGIYHDQ
                     VLNGTLLQHIGLKPPFQETVVISNTRLDQPLPVGTTPAAAASNAAPNLRAIQADWKTP
                     YMQHWSLDVQHQLTAKTVLSVGYFGSKGTHMIGAFELNELAPGQALDSQCATGAPTTP
                     SVPCLVPGTAFFSSAATNILDQIRPFRGYRTITMIQPRFNSNYHSMQFSAQQRFSGAS
                     QVNVAYTWAKNLTDNQTDRSTAPQNSADISIERGRATLDRRHVLTLNYIYELPFFKNQ
                     QGFVGKALGGWQASSIVNMQSGLPFTPATSNFDPAGLGFIPALVAGGRPNVICDPNAN
                     APHTLERFFDTSCLQANPLAMATNIPNVIGNAGRGIINGPPTYRVDFPMTKNIRFGEN
                     VRVQLRGEAFNVFNHTNVRTINTNVTQAAYGTVTAVRDPRIIQLGAKLNF"
     gene            complement(3277..3429)
                     /locus_tag="AC159P_21065"
                     /pseudo
     CDS             complement(3277..3429)
                     /locus_tag="AC159P_21065"
                     /inference="COORDINATES: protein motif:HMM:NF051698.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
     gene            complement(3505..3741)
                     /locus_tag="AC159P_21070"
     CDS             complement(3505..3741)
                     /locus_tag="AC159P_21070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3697994.1"
                     /translation="MRNHLRIARRAAALLVALCCVLLGPAVGAQEINGAIVGTIKDAN
                     GASVGGATVTVTDANTKVDVRTFRRSPTSSSATT"
     gene            complement(3974..5296)
                     /locus_tag="AC159P_21075"
     CDS             complement(3974..5296)
                     /locus_tag="AC159P_21075"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014675.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 10-like protein"
                     /protein_id="HIR3697995.1"
                     /translation="MLNRRVRPPKPWLLGLCVVSCVALLSTPARAQDAAPTPTPAPKV
                     AAPPAGEVRALWVVRTTLVSKKSIEEMVERAAANGFNTLIVQVRGRGDAYYRSQWEPR
                     AVTLKEQPTDFDPLAHTIKEARRKKLKVHAWVNTNLLANLDELPVEPAHVYNAHPEWL
                     AVPRAVAAELYSMPPRDPRYRASIVEWSKANRAELEGVYVAPARAEVREHVYSIWMDL
                     LARYEVDGLHFDYVRFASPDFDYSRASLERFRAWLDPHLSSEERRLLDRALQSDPLAA
                     ADSYRERFADFQREQVTVLVERIFHGVKKRRPGTIVSAAVFANDENARERRFQDWRRW
                     LALGLLDVVCPMAYSTDTEVFRKQIEVARAAANVSGKRVWAGIGAYRVTPESAVEKIN
                     AARAAGADGVILFSYDSATKTGPLNPSGDYLERVRRAAFDPPPRAAAP"
BASE COUNT          800 a         1710 c         2071 g          905 t
ORIGIN      
        1 aaccgctcct tcttcctcaa cgagctggac gggttcgcgt cgaacacggg cgtcgtcgtc
       61 gtggcgacga cgaaccaccc ggagcggctc gacccggcca tcctcgaccg cccgagccgc
      121 ttcgaccgca agttctactt cgagctgccc gccgccgaag tgcggctgct ctatgcgcgc
      181 cgctggaacg agctgctcca gcccgagacg cgtctcggcg agggcgccct cgccgaaacg
      241 gcggcgcgca cggagggctt ctccttcgcg tacctgaagg agctgttcgt cgcctctctg
      301 atggagtgga tgaacgcggg cggcgcgcgc tcgatgggag aggtgttggc ggagcgcacg
      361 gcgcggctga tcgagcagat gagcagccgc gacgacgacg gcggcaagga cgcgggcgag
      421 gaaggcgggt cgaagggaga agacgaagac gaggagagcg acgacgacga gacggacgac
      481 gacgcggatc acatgacccg cgtcaacgtt cgcgcgcgcg gcgccgggca gggttagagc
      541 gcttcgtgcg cgacgcgccc gccgacgagc gtgaggcgcg ggcggccttc ggcgtcgagg
      601 gcgacgaggt ctgcgcgctt gccttcttcg acggagccgc acacggcgtc gaggccgagg
      661 aggcgcgcgg ggttgagcgc ggccatgcgg gcgaggtctg tctcggcgaa gccgaggcgg
      721 gagacgaggc gggcggcgtc gagcatggtg atgacggagc cggcgatggc gccgcgctcg
      781 ttcgaggtgc gccctccggc gaccgtgatg gtctcgcccc agacgcggta ctcgccgtcg
      841 ccgaggccgg cgggcgccac cgcgtcgctg atgagcgcga cgcgcgcggg cgtcttcgtg
      901 cgcaggacga gttcgaccat gacggggtcg ctgtggacgc cgtcggcgat cacgtccacg
      961 gtcacgtctt cgctgagcag gccccacgcg acggggccgg gcgcgcggtg gtggagcggc
     1021 ggcatcgcgt tcgggaagtg cgtcatgtgg cgcgcgcccg ccgcgcgcgc gcgttcgagc
     1081 atgtcgatgt cggcgcgcgt gtggccgacg gagacgaccc acccgcgacg gcgcagctcg
     1141 ccgatcagct cgacgccgcc cgccacctcg ggcgcgaccg tgatcatgtg cgccgcgcct
     1201 tccgcgttga cccgcggcag cgcctccacg tccgccgccg agtcgtaggt gcggaagaag
     1261 ggcacgcgca gcgccccgca ctgccgctcg ttgacgaagg ggccttcgta gtggacgccg
     1321 acggcgcgcg cggcggcggg gagcgcgtcc tgcgtccgca tcagttcttc gatggcacgg
     1381 gcggcgcggg cgtaatcctc ttcgggcgcg gggacgaagg tcgggagcca ggcggtgacg
     1441 ccgcgcgagg cgaggaagcg cgcgaccgcg tgtaggtcgt ccgccgaagc ctccatcgtg
     1501 tccacgccga cggcgccgtg gatgtgcacg tccacgaagc cgggataaag agtcgtcccc
     1561 gcgagttcga tggtctgtag atgagtggca ccggcgctct catcaccttc gagcacgcgc
     1621 gcgatgcgcc cgccttcgac gagcaggctc gcgtcttcgg cgatgcgcgc gggcagcacg
     1681 aggcgcgcgt ggcggagcag aaggcgttcg ggcgtcgctt cgttgttcgt catcccggca
     1741 ttatagcgac gcgcggctga gactgaccac cgtccctttg tgcgcgggcg gcgcgtcgag
     1801 cgtacgggcg aacgcccgcg ccggggtggt ttgtcggcgc gggcgtcggt gtgtttgagc
     1861 ggtcggcttt agaagttgag cttcgccccg agttggatga tgcgcgggtc gcggacggcg
     1921 gtgacggtgc cgtaggcggc ctgcgtgacg ttggtgttga tggttctgac gttggtgtgg
     1981 ttgaagacgt tgaatgcttc gccgcggagc tgcacgcgga cgttctcgcc gaagcggatg
     2041 ttcttcgtca tagggaagtc cacgcggtag gtcggcgggc cgttgatgat gccgcgaccg
     2101 gcgttgccga tgacgttcgg gatgttggtg gccatggcga gcgggttggc ctgtagacag
     2161 gaggtgtcga agaaccgctc cagcgtatgc ggcgcgttcg cgttcgggtc gcagatgacg
     2221 ttcggcctgc cgcccgcgac gagcgccggg atgaagccga ggcccgccgg gtcgaagtta
     2281 gaggtcgcgg gcgtgaacgg caggcccgac tgcatgttga cgatgctgga cgcctgccag
     2341 ccgccgagcg ccttaccgac gaagccctgc tggttcttga agaaaggcag ctcgtaaatg
     2401 tagttgaggg tgaggacgtg acggcggtcg agcgtcgcgc ggccccgctc gatactgatg
     2461 tcggcggagt tctgcggcgc ggtcgagcgg tcggtctggt tgtcggtcag gttcttcgcc
     2521 cacgtgtagg cgacgttgac ctgcgaggcg ccgctgaagc gctgctgcgc cgagaactgc
     2581 atgctgtggt agttcgagtt gaagcgcggc tggatcatgg tgatcgtccg gtagccgcgg
     2641 aaggggcgga tctggtcgag gatgttggtc gccgccgagg agaagaaggc ggtgccgggc
     2701 accaggcagg ggaccgacgg cgtggtcggg gcgccggtcg cgcactgcga gtcgagcgcc
     2761 tgaccgggcg ccagctcgtt cagctcgaac gcgccgatca tgtgcgtgcc cttcgagccg
     2821 aagtagccga ccgacaggac ggtcttcgcc gtcaactggt gctgcacgtc gagcgaccag
     2881 tgctgcatgt agggcgtctt ccagtcggcc tgaatggcgc gcaggttcgg ggccgcgttc
     2941 gaggccgccg ccgccggggt cgtccccacg gggaggggct ggtcgagccg cgtgttcgag
     3001 atgaccaccg tctcctggaa gggcggcttc aggccgatat gttggagcag cgtcccgttc
     3061 agcacttggt cgtggtagat gccgtagccg gtgcggacgg cggtgcgccc cttgccgaac
     3121 gggtcccacg cgaggccgac gcgcggcgcg aagtcgcgct tcgagacctc gaagacggac
     3181 tcgccgaagg gcgagatggt gggcgtgcaa gttcggggcc gactggaagt tctgcgagtt
     3241 gacgatgagg ccggagcaga agttgcccgt gccggggacg cggttgcccg cgcccgtcac
     3301 ctgcggcgcg ttggcgcggt tgtagagttc gggcacgaag ttggtgagcc gcccgttctt
     3361 gtcgcgcggc gggccgtagt aggagtggcg caggccgtag tagagcgtca ggttcgggcg
     3421 ggcgcgccac tcgtcctgcg cctaggtctc ggacggcctt ctgccgcagg tcagccgtgt
     3481 agtcgaagcg ggcctgcgtg aaggtcacgt tgttgccgag gaggaagttg gcgaacgcct
     3541 gaaagttcgc acgtccacct tcgtgttcgc gtccgtgacc gtcaccgtcg cgccgccgac
     3601 gctggcgccg ttcgcatcct tgatcgtgcc gacgatggcg ccgttgatct cctgcgcgcc
     3661 gacggcgggg ccgaggagca cgcagcatag cgcgacgagc agtgccgccg cgcggcgtgc
     3721 aatccggaga tgatttctca tgggatgttt ccttttcgtt tcggcagaag attacggcct
     3781 cgcgccggac agtccgagac gccccgttcg tgtccccggc gtcgtttcct gtgatgttga
     3841 caagtgtgac ctgatacgac tcgagtcccg gctgaggctc aatgtattga cttatacacc
     3901 cgccgttatc tcttcgcaag aatcttcatc cattcgattg aacgcgcgcc gaaccgtcac
     3961 ggcgcgcgcg ctttcaaggc gcggcggcgc gcggcggcgg gtcgaacgcg gcgcggcgga
     4021 cgcgttcgag gtaatcgccc gaggggttga gcggccccgt cttcgtcgcc gagtcgtagg
     4081 agaaaaggat cacgccgtcg gccccggcgg cacgcgcggc gttgatcttc tcgaccgccg
     4141 attcgggcgt cacgcggtag gcgccgatgc ctgcccaaac gcgcttgccg gagacgttgg
     4201 cggcggcgcg cgccacctcg atctgcttgc ggaagacttc ggtgtcggtc gagtaggcca
     4261 tcgggcagac cacgtcgagc aggccgagtg cgagccagcg gcgccagtcc tgaaagcggc
     4321 gctcgcgcgc gttctcgtcg ttggcgaaga ccgccgccga gacgatcgtt ccgggccggc
     4381 gcttcttgac gccgtggaag atgcgctcga cgagcacggt gacctgctcg cgctggaagt
     4441 cggcgaagcg ctcacggtag gagtcggcgg cggcgagcgg gtcgctctgg agcgcgcggt
     4501 cgaggaggcg ccgctcttcc gaggagaggt gcgggtcgag ccacgcgcgg aagcgttcga
     4561 gcgaggcccg gctgtagtcg aagtcggggc tggcgaagcg gacgtagtcg aagtgcaggc
     4621 cgtccacctc gtagcgcgcg agcaagtcca tccagatcga gtagacgtgc tcgcgcacct
     4681 ccgcgcgcgc gggggcgacg tagacgcctt cgagttccgc gcggttggcc ttcgaccact
     4741 cgacgatcga cgcgcggtag cgcgggtcgc gcggcggcat cgagtagagt tcggcggcga
     4801 cggcgcgcgg cacggccagc cattcggggt gcgcgttgta gacgtgcgcg ggttcgacgg
     4861 gcagctcgtc gaggttggcg aggaggttcg tgttgaccca cgcgtgcacc ttcagcttct
     4921 tgcgccgcgc ctccttgatg gtgtgggcga gcgggtcgaa gtccgtgggc tgctccttca
     4981 gggtgacggc gcgcggctcc cactgcgagc ggtagtaggc gtcgccgcgc ccgcgcacct
     5041 gcacgatgag cgtgttgaag ccgttcgcgg cggcgcgctc gaccatctct tcgatactct
     5101 tcttcgagac aagcgtcgtg cgcacgaccc acagcgcgcg cacctctccg gcgggcggcg
     5161 cggcgacctt tggcgcgggc gtcggcgttg gcgccgcgtc ctgcgcgcgc gcgggcgtcg
     5221 aaagcagtgc cacgcacgac acgacgcaca ggcccaacag ccacggcttc ggcggacgga
     5281 ctctccggtt gagcatgaaa atttttattg cggaacgcgc gtgataactg taggggcagg
     5341 gcttgtcctc ggccccccaa ccgtcgatga aagcaacgtc ggacggactc ataaccgggg
     5401 ggccgaggac aagccctgcc cccacagcct tcatgaacgt tgcacactca ttcgtagccc
     5461 gctcgtcgct ttcctttcgc ctcgta
//
LOCUS       DBNGOH010001079         1575 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_5112925, whole genome shotgun sequence.
ACCESSION   DBNGOH010001079 DBNGOH010000000
VERSION     DBNGOH010001079.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1575)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1575)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1575
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_5112925"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1050)
                     /locus_tag="AC159P_21080"
     CDS             complement(<1..1050)
                     /locus_tag="AC159P_21080"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF018076.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HIR3697996.1"
                     /translation="MWYALDITLAARAREAVEYALMEAGALGTEVVAEDAEGARVAAY
                     FDAPPQVEAVRAALLEAVRIYGLASSDVREMRLREVADADWLAEWKKGWRATEVGERF
                     VVAPPWSEVESGSERIVIRIEPGMAFGTGTHETTRLCLALVEKLFRGESFLDVGTGTG
                     ILAIAAAKLFPGARVEACDTDPLAVEVARENATLNGVTEVIDFRVGSVNEFDGGAFDG
                     RSSPQAETVCANLTADVIVPLLPSLLAAAREKLILSGILETQVEAVRSRLRELGVDGC
                     GVVRDGEWVALAVVLPSGPGPSDEGGPRAMETIILPPERLSVGPTVHLVYLRSGGMVV
                     TKKPYSGWREIQDEYE"
     gene            complement(1291..>1575)
                     /locus_tag="AC159P_21085"
     CDS             complement(1291..>1575)
                     /locus_tag="AC159P_21085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020716534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HIR3697997.1"
                     /translation="EIPYVTAVVTEKYDQEREDFARINCAIFVERASQKKIIIGKGGE
                     RIKQIGTAARRDIERLVGHRVHLELFVKVEEDWRNSPRALSEMGIEDRKP"
BASE COUNT          270 a          576 c          471 g          258 t
ORIGIN      
        1 ttcatactcg tcctgtatct cgcgccagcc ggaataaggc ttcttggtga cgaccatccc
       61 gcccgacctg agatacacga gatgcacggt cggcccaacg gacagcctct ccggcggcag
      121 gatgatcgtc tccatcgccc gcggcccgcc ctcatccgac ggccccggcc cggacgggag
      181 cacgaccgcc agggccaccc actcgccgtc ccgcacgacc ccgcaaccat cgacgccgag
      241 ttcgcgcagg cgcgagcgca ccgcctcgac ctgtgtttcg aggatgccgg agaggatcag
      301 cttctcgcgc gccgcggcga gcagcgacgg cagcagcggc acgatcacgt ccgccgtcag
      361 gttcgcgcag accgtctccg cttgaggaga cgacctcccg tcaaacgcgc ctccgtcaaa
      421 ttcgttgacc gacccgaccc ggaagtcgat gacctcggtg acgccgttga gcgtcgcgtt
      481 ctcgcgcgcc acctcgacag ccagcgggtc cgtgtcgcag gcttcgacgc gcgcgcccgg
      541 aaaaagcttg gcggcggcga tggcgaggat gcccgtgccc gtgccgacat cgagaaagct
      601 ctcgccgcgg aaaagttttt cgaccagcgc gaggcacaac cgcgtcgtct cgtgcgtgcc
      661 cgtcccgaag gccatgcccg gctcgatgcg gatgacgatg cgctcgctcc cgctctcgac
      721 ctcgctccac ggcggcgcga cgacgaaccg ctcgccgacc tccgttgcgc gccaaccctt
      781 cttccactcc gccagccagt ccgcgtccgc cacctcgcgc aagcgcatct cgcgcacgtc
      841 cgacgacgcg agtccgtaaa tacgcaccgc ctccagcagc gccgcgcgca ccgcctcgac
      901 ctgcggcggc gcgtcgaagt acgccgcgac gcgcgccccc tccgcgtctt ccgcgaccac
      961 ctccgtcccc agcgcgcccg cctccatcag cgcgtactcc accgcctccc gagcgcgcgc
     1021 cgccagcgtg atgtcgagtg cgtaccacat aggcgaaagc cgtgaaccgt gaaccgtaaa
     1081 tcgtgacaag aagaaagccg tgcgccgtga gctgtgaacc gtgacaagtc aaagaagtga
     1141 tgaggactga ggactgagga ctgaggactg agtgatgaga aacaaaggcg cgcgccgtca
     1201 gccgtgacga gtccgaacag cacccctctc atcattcgtc atccatcgcc gatcatggct
     1261 tttcactcgt cacggttcac ggttcacggt tcacggttta cggtcttcga tccccatttc
     1321 gctcagggcg cgcggcgagt tgcgccagtc ctcttcgacc ttgacgaaga gttcgaggtg
     1381 gacgcggtgg ccgacgagcc tctcgatgtc gcggcgggcg gcggtgccga tctgcttgat
     1441 gcgctcgccg cccttgccga tgatgatctt cttctgcgac gcgcgctcga cgaagatcgc
     1501 gcagttgatg cgcgcgaagt cctcgcgctc ctgatcgtac ttttcggtga cgacggccgt
     1561 cacgtagggg atttc
//
LOCUS       DBNGOH010001080         8177 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1264239, whole genome shotgun sequence.
ACCESSION   DBNGOH010001080 DBNGOH010000000
VERSION     DBNGOH010001080.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8177)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8177)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8177
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1264239"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="AC159P_21090"
     CDS             <1..213
                     /locus_tag="AC159P_21090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017298290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HIR3697998.1"
                     /translation="CGKESGLTSGVERFNCTLRQRVSRLVRKTLSFSKKLENHIGARL
                     CLFIRQAVHKSHEHAAYQPMYAPFDE"
     gene            complement(244..1158)
                     /gene="coxB"
                     /locus_tag="AC159P_21095"
     CDS             complement(244..1158)
                     /gene="coxB"
                     /locus_tag="AC159P_21095"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013033945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HIR3697999.1"
                     /translation="MSYHTAARQGHGAVRRVSGAGALVLLAGCGGVQSTLDPAGREAA
                     RIADLFWWMAAGSVVVWLAVIALALWAVRARPESHNLRRTRLLIVGGGAVVPTVVLTA
                     LLVYGLAPIPALLAPAPEGSLKVAVTGEQWWWRVRYHTNGGEEVTLANEIRLPVGHPV
                     EFRLDSPDVIHSFWIPPLAGKVDMIPGRVTRLVLRPTRTGVFRGVCAEYCGASHALMA
                     FNVVVQEREEFDRWLAGQAFFAHMTMHMGVVALAAPLVALGVAGARFDPVRRAPALFA
                     PVSASIFGSAQKVMLVREGASPPGCNGR"
     gene            2011..2457
                     /locus_tag="AC159P_21100"
     CDS             2011..2457
                     /locus_tag="AC159P_21100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010412712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIR3698000.1"
                     /translation="MQVKDIMTTDPACCTPDTTLQRVAEMMVEHDCGEIPVVENAASM
                     KPVGVITDRDITCRTVAKGLNPLTMAVSECMTTPCVTVTPDTPLDECCKVMEENRIRR
                     VPVVDAAGACCGIVALADIARHAAKRETAEVVKEVSEPSASASAAG"
     gene            2597..2830
                     /locus_tag="AC159P_21105"
     CDS             2597..2830
                     /locus_tag="AC159P_21105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007027288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3698001.1"
                     /translation="MAKCEVCGNAYYLSFEVVAAGARHTFDSFECAIHKPAPVCDHCG
                     CKIIGHGIEANGTFYCCANCARRKGGTQAADHV"
     gene            2881..3132
                     /locus_tag="AC159P_21110"
     CDS             2881..3132
                     /locus_tag="AC159P_21110"
                     /inference="COORDINATES: protein motif:HMM:NF025165.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase-like
                     domain-containing protein"
                     /protein_id="HIR3698002.1"
                     /translation="MAGDFRIFAGRANPRLARAGADELNVPLGACRVNSFPDGEVSVR
                     LDESVRGREVFIVQPTGPPVDDHLVELRRRAGKKEGEQR"
     gene            3129..3383
                     /locus_tag="AC159P_21115"
     CDS             3129..3383
                     /locus_tag="AC159P_21115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3698003.1"
                     /translation="MKRSYKVIAETSARMLEPLDPEKPIPTPPEPLPELPPTEPLPPD
                     PVPPPDPSPPSPVPPAPVPLPPIIVSRAPSPGRIAADSRV"
     gene            3645..4433
                     /locus_tag="AC159P_21120"
     CDS             3645..4433
                     /locus_tag="AC159P_21120"
                     /inference="COORDINATES: protein motif:HMM:NF051162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HIR3698004.1"
                     /translation="MKESWDTRIRRAEQLAARSEAARGLLTFYATLLRAQKGAYEYLR
                     GRKGWLPLGTLEEDLPFVRATMHGLLRAVEASGPPALVEEARALALACEDEIDGALLE
                     QWRAPSDTRFFAKALLQPYARRLAETGARPVGRELERREGRCPFCAGKPQVSFLRTTE
                     PGAESGGRGLVCSACLTVWPFRRVVCANCGEERPAKLGYFQSPEYEHIRIEACDTCNH
                     YIKGVDLTRLGLAVPVVDEVAAAALDLWALEHGYMKIELNLVGL"
     gene            4731..5306
                     /locus_tag="AC159P_21125"
     CDS             4731..5306
                     /locus_tag="AC159P_21125"
                     /inference="COORDINATES: protein motif:HMM:NF016750.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HIR3698005.1"
                     /translation="MDRRTFIKATLAGGGAVSAFGFDLAPVRAQTQGLKIARTSETRS
                     TCPYCSVSCGVIIHTLGDKSKNAVPQVVHVEGDPDHPINRGTLCPKGASLEQDILNER
                     RLTKPQVRRPGSDHWEDISWEQAFDEIARKVKRTRDQTFVERDGNGLTVNRCEGIGWT
                     GGCTDTNELNYLAVKVMRGMGVCYFETQARV"
     gene            5355..8096
                     /gene="fdnG"
                     /locus_tag="AC159P_21130"
     CDS             5355..8096
                     /gene="fdnG"
                     /locus_tag="AC159P_21130"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HIR3698006.1"
                     /translation="MTNGWIDIKNTDMMLIMGGNPAENHPCGFKWPVEAKKTRNAKMI
                     VVDPRFTRTAAVADLFLQIRAGTDIAFLGGLINYAIQQNRLARDYLVNYTNAAFVVKE
                     GFKLPEDGLFSGFDAQGTTYDTESWNYEAGGNVGPKAGGSNGGAAAPAGGTTSSETTG
                     EQQSAGQAPARSFEKQSPPVDSKPSTPPDQGQSAAGGVMSSQAGGGHQAAGPTGAAAQ
                     GGPKPPPQLPPNVAYDLSLEHERCVFQLLKRQFARYTPEMVERITGIPRDQFLKAADL
                     FTSVRKDGDMKRAATIIYAVGWTQHTFGTQIIRTAAILQLLLGNVGRAGGGVNALRGH
                     SNIQGATDMGGVFDIFPGYLKIPAPTDVDLATFLKRTTPTPSKPDAWDSFNYWSNTPK
                     FMVSFLKALYGDSAKKENDFNYHYLPKIDRKYSWTELFDCMYRGEVKGLFAFGMNAVA
                     IGPNSRKNIEALKRADWLVVCDIYPEETAEFWRAPGISKEEMAKIRTTVYRLPGAGFA
                     EKDGTFVNSARWLQWKHVALPPPGQARVDQEILARIFLKVRELYRAEGGKFADAISNL
                     SWSYTTPENPSLAEVAKEINGRALADLTDEKQPGVTLKAGQQLPGFAWLRDDGTTACG
                     NWLYSGSWTEAGPQMQRRGTEDPSGLGIFPGWAWSWPANRRVMYNRASCDPSGKPWDA
                     ERRQVWWDEAQQKWVGNDVPDFKPDSNPSDHMGPFIMNPEGVGRIFAPLAAFADGPFP
                     EHYEPVESPVANSLHPGQSNNPVAKVYKSDADKLGTPAEGFNVVCTTYRLTEHYHYWT
                     KNNPMNVQLVPEPFVEIPAALADEMGLRGGELVRVSSARGQYVAKAMVTKRIKPMMID
                     GKKTYQIGIPIHLGYRGIKEDEGKTALDPANQLSPAAIDPNAYTPEYKGFLVKIEKA"
BASE COUNT         1558 a         2631 c         2687 g         1301 t
ORIGIN      
        1 tgcggcaagg agtccggact gacgagcggg gtcgagcgct tcaactgtac gctgcggcag
       61 cgggtctcgc ggctggtacg caagacgctc tcgttttcaa agaagcttga gaaccacatc
      121 ggggcgaggc tttgcctatt tattcggcag gctgtgcata aaagccacga acatgcagca
      181 tatcaaccga tgtatgcgcc ttttgacgaa taaataggca aagcctcggg gcgatcaagt
      241 atttcatctg ccattacaac cgggaggtga tgcgccttct ctgaccagca ttaccttttg
      301 agcactaccg aaaatcgaag ccgatacggg cgcgaacagc gcgggcgcgc ggcgcacagg
      361 atcgaaacgc gccccggcga cgccgagcgc gacgagaggg gccgccaacg ccacgacgcc
      421 catgtgcatc gtcatgtgcg cgaagaaggc ttgcccggcg agccagcggt cgaactcctc
      481 gcgctcctgc accacgacgt tgaaggccat cagcgcgtga gaggccccgc agtactcggc
      541 gcagacgccg cggaagacgc ccgtccgcgt cggcctcagc acgagccgcg tcacgcggcc
      601 cggaatcatg tccaccttgc ccgcgagcgg gggaatccag aaggagtgaa tcacgtccgg
      661 gctgtcgaga cggaactcca ccgggtggcc gacgggcagg cgaatctcgt tcgccaacgt
      721 cacctcctcg ccgccgttcg tgtgatagcg cacgcgccac caccactgct cgcccgtgac
      781 ggcgaccttc agactccctt ccggcgcggg ggcgaggagc gcggggatcg gggccagccc
      841 gtagacgagc agcgcggtca gcaccacggt cggcacgacc gcgccgcccc cgacgatcag
      901 aagccttgtc cggcgcaggt tgtgagactc gggccgcgcg cggacggccc agagggcgag
      961 ggcgatgacg gcgagccaga cgacgaccga cccggcggcc atccaccaga agagatcggc
     1021 gatgcgcgcg gcctcgcgcc ccgccgggtc gagcgttgat tggacgccgc cgcaaccggc
     1081 cagcagcaca agcgcgcccg cgccgctcac gcgccggacg gcgccgtgcc cctgtcgcgc
     1141 cgccgtgtgg taagacaatc ttcgcaggtg tcgagcgcca agtctcaccg gcaacacctc
     1201 gcaaaccttc accgtcagca aaggttgtcg agaccttctt gcgcccttcg cgccttcgcg
     1261 tgagatttcc ccgctcagat aggggaactc gggcgaaggc gcgaagggtt ccgaagcgag
     1321 gacggttcag gcagacctcg cccgtcgctg tgcgcggctc atcctcccct tccatcgtcg
     1381 tcgagggata ggcgaatgat cgttgtcgct tgaattgtat tggctggggg aaaatcgaac
     1441 aacagacacg cgaactacga ggcttgattc tcgtccttct cgcctctcta tcgcgggcgg
     1501 tctttttaag gggaagcggg cgcatcatac tctctttaca gtggaaggta caatcagtac
     1561 cttcaaggag agtgacggtc tgcgcgctca ctgcggcgac cgcggacttc actccctgcg
     1621 ctccgagctt gggggcgggt gcgctgtgat tttctttccg cctttggtac aatctgtaca
     1681 cacaaaatta ttcccccatc gtgtatgagg tcagcccgtt gacggagcag gccggagtag
     1741 ttttctctcc tcaagcctgc cggtcgggcg gattcttctt ttgccgtgcg gcgtaagggg
     1801 cgagcacgcc gactccacgg gcacagcggg agccgcgggt gcggcgggcg caggggcaat
     1861 gggaggcaca agagctaaga cgatctgacg atatttcggc gcggtgaacg aagtctcgct
     1921 cgactgtgcc ggtgcatgga gcgtcccgcc ggagtatcgg cgggacgctt tacttagtga
     1981 tagttggttt gctgttaaag gagaagcacc atgcaagtga aagacatcat gacaaccgac
     2041 ccggcgtgtt gcacgccgga taccacgctc cagcgcgtcg ccgagatgat ggtggagcac
     2101 gactgcggcg agatacccgt cgtcgagaac gcggcgagca tgaagccggt cggcgttatc
     2161 accgaccgcg acatcacctg ccggacggtg gcgaagggac ttaacccgct gacgatggcg
     2221 gtctcggagt gcatgacgac gccgtgcgtc accgtgacgc ccgacacgcc gctcgacgag
     2281 tgctgcaagg taatggagga gaatcggatc aggcgcgtgc cggtcgtgga cgctgcgggg
     2341 gcctgctgcg gcatcgtcgc gctcgccgac atcgcgcggc acgccgcgaa gcgcgagacc
     2401 gccgaagtgg tcaaggaggt gtccgagccg agcgcgtcgg cgtcggcagc aggctgaacg
     2461 aacatgccgc gcggttttca ccggtgcgct tccaacggcg gcacgaaccg gtgccgcttc
     2521 caaacgccga accgcttgga ggcccggcca cgtggccggg cctccaccgc ccgacgacac
     2581 ggaaggagtg aatgggatgg cgaagtgcga ggtgtgcggc aacgcttatt acttgtcctt
     2641 cgaggtcgtc gcggcgggcg cgcgccacac gttcgacagc ttcgagtgcg cgatacacaa
     2701 gccggcaccc gtgtgcgacc actgcggctg caagattatc ggccacggca tcgaggcgaa
     2761 cggcaccttc tactgctgcg ccaactgtgc ccgccggaag ggcgggacgc aggcggcgga
     2821 tcacgtctga cagtcgtgag tggctggcgc cgacgcgatg tgtgaaaggt cgcattcgac
     2881 atggcgggcg acttccgtat tttcgccggc agggccaatc cgcgtttggc gcgggcgggc
     2941 gcggacgagc tgaacgtgcc gctcggggcg tgccgcgtca acagcttccc ggacggcgaa
     3001 gtgtcggtca ggctcgacga atcggtgcgc gggcgcgagg tcttcatcgt gcagccgacg
     3061 gggccgccgg tggacgacca cctcgtcgag ctgcgccgcc gcgccggcaa aaaggagggt
     3121 gaacaacgat gaagcgtagc tataaggtga ttgccgaaac gtcggcgcgt atgctggagc
     3181 cgctcgaccc ggagaagccc atccccactc cgcccgagcc gctgccggag ctgccgccga
     3241 ccgaaccgct cccgcctgac ccagtgcccc cgcccgaccc gtcgccgccg tcacccgtgc
     3301 cgcccgcccc tgtgcccctg ccgccgatca tcgtttcgcg cgccccctca ccggggagga
     3361 tagccgccga ctcacgagtt tgaggcggtc gtcctcaccg tcggactgat catgttgggc
     3421 gtcgggctgt tgagaagagc gtaatggccg gtagcccgga gtgaggagga tcggaatgaa
     3481 gatacttctg gccgttgatg cttcggagta ttcggcggat gccgtgagag aggtcgcgga
     3541 gcgtccgtgg ccgcccgcta cgacggtgcg cgtgctctcg cccgcctgac gccggcatcg
     3601 accggcgcac gttcgacagc cctccggcgg cttgagggag cgtgatgaag gaatcgtggg
     3661 acacacgtat acggagggcc gagcaactcg ccgcacggag cgaagcggca aggggactgc
     3721 tgacgtttta cgcgacgctg ttgcgcgcgc agaagggtgc ttacgaatat ctccgcggtc
     3781 gcaaggggtg gcttcctttg ggcacgcttg aggaagattt gccgttcgtg agggccacga
     3841 tgcacggcct gctgcgcgcc gtcgaggcga gtgggcctcc ggccttagtc gaggaggcgc
     3901 gggcactcgc gctcgcgtgc gaagacgaaa tagacggggc gctactcgaa caatggcgtg
     3961 cgccgtcgga cacacggttc ttcgcgaagg cgttgttgca accctacgcg cgccggctcg
     4021 cggagacggg cgcgcggccc gtcggtagag agttggagag gcgggaaggc cgttgcccat
     4081 tctgcgcggg caagccgcag gtcagcttcc ttcggacaac agagccgggg gcggagagcg
     4141 gtgggcgggg cctcgtctgt tcggcgtgtc tgaccgtctg gcctttccgt cgcgtcgtct
     4201 gcgcgaactg cggcgaggag cgaccggcga aactcggata ttttcaatcg ccggagtacg
     4261 agcacatcag aatcgaagcc tgcgacactt gcaaccacta catcaagggg gttgacctga
     4321 cgcggctcgg cctcgccgtc cccgtggtgg acgaagtcgc ggcggcggcg ctcgacctgt
     4381 gggcgctcga acacggctac atgaagatcg agttgaatct ggtgggtctg tgatagcgag
     4441 tcggctcaag gccggaaact tcgggggctg gaaaaagctt gctcggagaa ggccgttaat
     4501 gaatcgagtc tccattgaga gcgcgaattt cgcgactccg cgcagggctg gcccgacact
     4561 tttagggagc gccctaaata cagttgacat ccgcccccgt caggcatatt ttttacctaa
     4621 tccacaaccc ctaagagata ttttcagccc catcgcagtc gtccgttcga cgcgcgcgcc
     4681 cctgcgcccg ctgattcatg ccccctgctc tctgcgagga gaaaaatggt atggaccgca
     4741 gaactttcat caaggcgacg ctggccggag gcggcgccgt ctcggccttc ggcttcgacc
     4801 tcgcgccggt gcgcgcgcag acgcagggac tcaagatcgc gcgcacgagc gagacgcgct
     4861 cgacctgtcc ctactgctct gtgagctgcg gcgtcatcat ccacacgctc ggcgacaagt
     4921 cgaagaacgc cgtcccgcag gtcgtacacg tcgagggcga ccccgaccac ccgatcaacc
     4981 gcggcacgct ctgcccgaag ggcgcttcgc tcgaacagga catcctcaac gagcgccgtc
     5041 tgacgaagcc gcaagtgcga cggcccggct ccgaccattg ggaagacatc tcttgggagc
     5101 aggcgttcga cgagatcgcg cggaaggtta aacggacgcg cgaccagacg ttcgtcgagc
     5161 gtgacggaaa cggtctgacg gtcaaccgct gcgagggcat cggctggacg ggcggctgca
     5221 ccgacacgaa cgagctgaat taccttgcgg tcaaagtcat gcgcgggatg ggggtctgct
     5281 acttcgagac gcaggcccgt gtttgacacg ggccgacggt ctccagtttg gggcctacct
     5341 tcggacgcgg cgcgatgacc aacgggtgga tcgacatcaa gaacaccgac atgatgttga
     5401 tcatgggggg aaaccctgcc gagaaccacc cctgcggctt caagtggccg gtcgaggcca
     5461 agaagacgcg caacgccaag atgatcgtcg ttgaccctcg gttcacgcgc accgcggcgg
     5521 tcgccgacct cttcctgcaa atccgcgcgg gcacggacat cgccttcctc ggcggcctca
     5581 tcaactacgc catccagcag aaccgtctcg cgcgcgacta tctcgtcaac tacacgaacg
     5641 cggccttcgt cgtcaaagaa ggcttcaagc ttcccgaaga cggtctcttc tccggcttcg
     5701 acgcgcaagg tacgacctac gacacggagt cctggaatta cgaggcgggc gggaacgtcg
     5761 gcccgaaggc cgggggaagc aacggcggcg ctgccgctcc cgcgggcggc acaacttcga
     5821 gcgagacgac cggcgagcag caaagtgcgg ggcaagcgcc tgcgaggagt ttcgagaaac
     5881 agtcgccgcc ggttgactcc aaaccctcga cgcctcccga tcaggggcag tcagccgccg
     5941 ggggcgtgat gagcagccag gcaggcggcg ggcatcaagc cgccggcccc acgggcgcgg
     6001 cggcgcaggg cgggccgaag ccgccgccac agctcccgcc caacgtcgct tacgatcttt
     6061 cactcgaaca cgagcgctgc gtcttccaac tgctcaagcg acagttcgcg cgctacacgc
     6121 ccgagatggt cgaacgcatc acgggcatcc cgcgcgatca gttcctcaag gccgcagacc
     6181 tcttcacctc cgtgcgcaag gacggcgaca tgaaaagggc ggcgacgatc atctacgccg
     6241 tcggctggac gcagcacacc ttcggcacgc agatcatccg cacggcggcc atcctgcaac
     6301 tgctcctcgg caacgtcggg cgcgcgggcg ggggagtgaa cgcactgcgc ggccattcca
     6361 acatccaggg cgcgaccgac atgggcggcg tcttcgacat cttccccggc tacctgaaaa
     6421 tcccggcccc cacagatgtt gacctcgcca cgttcctgaa gcgcacgacg cccacgccgt
     6481 cgaagcccga cgcgtgggac tcgttcaact actggtcgaa cacgccgaag ttcatggtct
     6541 cgttcctcaa ggcgctgtac ggcgactcgg cgaagaagga gaacgatttc aactatcatt
     6601 acctgccgaa gatcgaccgg aagtattcgt ggacggagtt gttcgactgc atgtaccggg
     6661 gcgaggtcaa gggactcttc gccttcggca tgaacgccgt cgccatcggc ccgaactcgc
     6721 gcaagaacat cgaggcgctg aagcgggcgg actggctcgt cgtctgcgac atctaccccg
     6781 aggagacggc tgagttctgg cgtgcgcccg gcatctcgaa ggaggagatg gcgaagattc
     6841 ggacgaccgt ctaccgcctg cccggcgcgg gcttcgccga gaaggacggc acgttcgtca
     6901 actcggcccg ctggctccag tggaagcacg tcgccctgcc gccgcccggt caggccaggg
     6961 tggatcagga gatactggcg cgcatcttcc tgaaggtgcg cgagctttac agagcggagg
     7021 gcggaaagtt tgcagacgcg atttcgaacc tctcctggtc ttacacgacg ccggagaatc
     7081 cttcgctcgc ggaagtggcg aaagagatta acgggcgcgc gctcgcagac ctcacggacg
     7141 agaagcagcc cggcgtcacc ctcaaggccg gtcaacaact gccgggcttc gcgtggctca
     7201 gagacgacgg cacgaccgcg tgcggcaact ggctctattc ggggtcgtgg acggaggccg
     7261 gcccgcagat gcagcggcgc ggaaccgaag acccttcggg cctcggcatc tttcccggtt
     7321 gggcttggtc ttggcccgcg aaccgccgcg tgatgtacaa ccgcgcgtcg tgcgatccgt
     7381 ccggcaagcc ctgggacgcc gagcgccgac aggtctggtg ggacgaggcg caacagaagt
     7441 gggtcggcaa cgacgtgccc gacttcaagc cggactcgaa cccttcagac cacatggggc
     7501 cgttcatcat gaaccccgaa ggcgtcggga gaatcttcgc gccgctcgcc gccttcgccg
     7561 acgggccttt ccccgaacac tacgagccgg tcgagagtcc cgtcgcgaac tccctgcacc
     7621 ccggccaatc gaacaacccg gtcgccaaag tctacaagtc ggacgccgac aagctgggca
     7681 cgcccgccga gggcttcaac gtcgtctgca ccacctaccg actgaccgag cactaccact
     7741 actggacgaa gaacaacccg atgaacgtcc agctcgtgcc cgagccgttc gtcgaaatcc
     7801 ccgccgcgct ggccgacgag atgggcctgc gcggcggcga actcgtgcgc gtgtcgagcg
     7861 cgcgcgggca gtacgtggcg aaggcgatgg tgacgaagcg catcaagccg atgatgatcg
     7921 acggcaagaa gacatatcaa atcggcatcc cgatccactt gggctatcgc ggcatcaaag
     7981 aggacgaggg caagaccgcg ctcgacccgg ccaaccagct ctcgcccgcc gcgatcgacc
     8041 cgaacgccta cacgccggag tacaagggct tcctcgtgaa gatcgagaag gcttgagggg
     8101 agggaacgag tgtgagaggc gagaaacttt atcaaatcca gcgcatctcg gcccaccccg
     8161 gcaccgtccc cggtcgg
//
LOCUS       DBNGOH010001081         4651 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4014238, whole genome shotgun sequence.
ACCESSION   DBNGOH010001081 DBNGOH010000000
VERSION     DBNGOH010001081.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4651)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4651)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4651
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4014238"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..796
                     /locus_tag="AC159P_21135"
     CDS             <1..796
                     /locus_tag="AC159P_21135"
                     /inference="COORDINATES: protein motif:HMM:NF016836.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HIR3698007.1"
                     /translation="RANNRAADFRGKVSSAVRAVRPRENVWAARGRRQEVTKEVFEVM
                     QARTAKEADKELRDRVERALDWNPAVKSTDIGVAAQDSTVTLTGFVQSYAEKLAAEKT
                     TKRTYGVRAVANDLQVKPFSRKTDTDIAAAAVVALDARVDVPDEKIKVTVKGGWVYLE
                     GKVEWRFEKEAAESAVKHLAGVVGVTNNIEIKPRVSTTEVKSKIEEALKRSAELDARR
                     ITVSAHDGTVELWGNVRAWMEREEAERAAWAAPGVHSVVSHISIVP"
     gene            925..1284
                     /locus_tag="AC159P_21140"
     CDS             925..1284
                     /locus_tag="AC159P_21140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3698008.1"
                     /translation="MKTEEQSEHWKAYVAEFNRQNKTRPSRLGIIKPGDRMEDYWLED
                     GLPFAAIGLENGGSAPVIEIMLGAEGDAERNVTHTVRGVKTLTLRLTDDGSDDGLDVQ
                     DAEGATTLLRFEPSTEG"
     gene            1370..1735
                     /locus_tag="AC159P_21145"
     CDS             1370..1735
                     /locus_tag="AC159P_21145"
                     /inference="COORDINATES: protein motif:HMM:NF036463.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5335 family protein"
                     /protein_id="HIR3698009.1"
                     /translation="MITKEIPRERWAAFCDDFSRMNEDLPVTVEVLSAEIGDQTEALW
                     IPLRGVTAELNGGCGDMIEIMLGDAAEAHVTHAVTRPASVRLAATEAGGPQALEIESE
                     GGVTTLLRFRPLTHAVGAG"
     gene            1764..2027
                     /locus_tag="AC159P_21150"
     CDS             1764..2027
                     /locus_tag="AC159P_21150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3698010.1"
                     /translation="MRKEVLLAGCGVSLGAGLMYFLDPDRGSRRRAQLRDRTMHLARE
                     AADTLGKSGRDIRNRALGVVAEAGAHWRCEHVPDEVLAERDGE"
     gene            2104..>4651
                     /gene="fdhF"
                     /locus_tag="AC159P_21155"
     CDS             2104..>4651
                     /gene="fdhF"
                     /locus_tag="AC159P_21155"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HIR3698011.1"
                     /translation="MLRVRIDGLAHEFREGTTILDAARAVGARVPTLCDDERLRPRGL
                     CRLCLVEVKGRPHPCTACNTPLEEGMEVTTGGPRLERARRMILKILARDYPSDELHRL
                     PDKRLHRYLREYGFEDEMCGVADVDLVDDSHPYIHVDMSRCIDCRRCARICEEVQGQF
                     VWRVFNRGKAQRILPSSLKSLLESPCVSCGACSDTCPTGALADKSVLAFGEPNEWTKT
                     TCPYCGTGCELNVGRRDGRIVQILPVFDAPVSRGHLCVKGRYAFDFVRSADRVTEPMI
                     RRGGVWERVSWAEAVAFAAEGLRRVVERHGPDSVGVLGSARATNEENYLTQKFARVAL
                     GTNNVDCCARVCHTPTAAAMKLMLGTGAATNSFDDIERARAILVCGANPTESHPVVGA
                     RIKQAALRGAKLVVIDPRGIELARYAGQHLALRPGTNVPLLNAMAYTIIEEKLYDEEF
                     MRERVNGLEEFREFIRTYSPEAVAATCGVEANVIRETARVYATAKPAMCFHGLGVTEH
                     TQGTEGVMCLVNLALLTGNLGQPGSGINPLRGQNNVQGAAHMGCDPGTLTGSVPLAEG
                     KDSFERVWRAPVPMQRGMNLLEMMDAAGRGNLRALWAIGYDILLTNADAHATERALRS
                     LDLLVVQDLFMSETAKEFGTVFFPAASSFEKDGTFMNAERRVQRVRKAVEPPGHSKPD
                     WEIICLLARAMGKGDLFGYASPEEVWEEVRAVWPQGRGITYGRIERGGLQWPCPAEDH
                     PGTQVIHTESFPMGQRAALRRVPFRPTPETTDGEYPFLLITGRTLYPFNAGTMTMRTP
                     NASLRPADTLDMHPDDARRLALRKGERVRVRSRYGEAVLPINLTNAVKRGE"
BASE COUNT          868 a         1518 c         1588 g          677 t
ORIGIN      
        1 gcgagcaaac aaccgcgccg ccgatttccg cgggaaggtg tcgagcgcgg tgcgcgccgt
       61 ccgcccacgg gagaacgtgt gggcggcgcg cgggcggagg caggaagtta cgaaggaggt
      121 gttcgaagtc atgcaagcca gaacagctaa ggaagcggac aaagagctgc gcgacagggt
      181 cgagcgggcg ctcgactgga acccggcggt caagtccacg gacatcggcg tcgcggcgca
      241 ggacagcacg gtgacgctca ccgggttcgt ccagtcctac gccgagaagc tggccgccga
      301 gaagacgacc aaacggactt acggcgtgag ggccgtcgcc aacgaccttc aggtcaagcc
      361 cttctccagg aagaccgaca ccgacatcgc cgcggcagcc gtggtcgcgc tcgacgcgcg
      421 cgtggacgtg ccggacgaga agatcaaggt gacggtcaag ggcggctggg tctacctcga
      481 aggcaaagtc gagtggcgct tcgagaagga ggccgccgag tcggcggtca agcatctcgc
      541 gggcgtcgtc ggcgtcacca acaacatcga gatcaagccc agggtctcga cgaccgaggt
      601 caagtcgaag atcgaggaag cgctcaaacg cagcgccgaa ctcgatgcgc gccgcatcac
      661 ggtgtcggcc cacgacggga cggtcgagct gtggggcaac gtgcgcgcgt ggatggagcg
      721 ggaggaggcc gagcgcgcgg cgtgggccgc gcccggcgtc cacagcgtcg taagccacat
      781 ctcaatcgtg ccgtaaccgg cacaccaaca gcggcacggg gcggggcgtc atttcacccc
      841 ccttcatcat caccaagatg ctcccgcccc gcgcacgctc acagcgggag tgtccggcga
      901 cagagaccgg atgaggtgaa agccatgaaa acggaagagc agagcgagca ctggaaggcc
      961 tacgtcgcag agtttaaccg tcagaacaag acgcgcccct cccgcctcgg catcatcaag
     1021 cccggcgacc ggatggagga ttactggctg gaagacggcc tgcccttcgc ggccatcggc
     1081 ctggagaatg gaggcagcgc gccggtcatc gagatcatgc ttggcgccga aggcgacgcg
     1141 gaacgcaacg tcacgcacac cgtccgcggc gtcaagacgc tcacgctccg tctcaccgac
     1201 gacgggagcg acgacggcct tgatgtccaa gacgcggaag gcgcgacgac ccttctccgc
     1261 ttcgagcctt cgacggaggg gtgaacgacc atccgccgcc tgtctcgcgc gtcacgcgga
     1321 agtgagacaa ctttccccaa cgggaatctg gctgacgagg tgcacggaca tgattacaaa
     1381 agagattccg agagagcggt gggccgcatt ctgcgacgac ttcagccgca tgaacgaaga
     1441 tttacccgtc acggtcgaag tcctcagcgc ggagatcggc gaccagacgg aggcgctttg
     1501 gattccgctg cgaggcgtga cagccgagtt gaacggcggg tgcggtgaca tgatcgagat
     1561 catgctcggc gacgcggcgg aggcgcacgt cacacatgcc gtcacgcgcc cggcttccgt
     1621 ccgcctcgct gcgaccgagg cgggagggcc tcaggcactg gagatagagt ccgagggcgg
     1681 ggtgacgaca ctcctccgct tccgcccgct cacgcacgcc gtcggagctg gttgacggcg
     1741 gagccgggga aggaaggtgt tgtatgagga aagaggttct gctggccggc tgcggcgtca
     1801 gcctcggcgc ggggttgatg tacttcctcg accccgaccg cgggagccga cgccgcgcgc
     1861 aactccgcga ccgcaccatg cacctcgcac gcgaggcggc ggacacgctc ggcaagagcg
     1921 ggcgcgacat ccgcaaccgc gcgctcggcg tcgtggccga agcaggcgcg cactggcgct
     1981 gcgagcacgt gcccgacgag gtgctggccg agcgcgacgg ggagtgaagg cgatgtatgc
     2041 cttcgccggt ttccttcggc aggtgctccc ggcgcgcgaa gtcatcgcgt gaggagagga
     2101 gacatgctac gcgtacgaat cgacggtctg gcgcacgaat tccgtgaggg tacgacgatc
     2161 ctggacgcgg cccgcgcggt cggcgcccga gtgccgacgc tgtgcgacga cgagaggctg
     2221 cggccccgcg gcctgtgtcg gctgtgcctg gtcgaagtga agggccgtcc gcacccctgt
     2281 accgcctgca acaccccgct tgaggaaggg atggaggtca cgaccggcgg cccacgcctc
     2341 gaacgcgcgc ggcggatgat cctcaagata ctggcacgcg actatccgtc cgacgagttg
     2401 cacaggttgc cggataaaag gctccaccgc tacttgcgcg aatacgggtt cgaggacgag
     2461 atgtgcggcg tggcggacgt tgatcttgta gacgactctc acccatacat ccacgtggac
     2521 atgtcgcgct gcatcgactg ccgtcgctgt gcgcgcatct gcgaggaggt gcaggggcag
     2581 ttcgtctggc gcgtcttcaa ccgcggcaag gcgcagcgca tcctccccag ctctctgaag
     2641 agcctgctcg aaagtccctg cgtcagttgc ggcgcgtgct cggacacctg cccgaccggg
     2701 gcgctggcgg acaagagcgt cctcgctttc ggcgagccga acgagtggac gaaaaccacc
     2761 tgcccttact gtgggacggg atgcgagctg aatgtgggca ggcgcgacgg gcggatcgtc
     2821 caaatcttgc ccgtgttcga cgcgcccgtc agccgcggcc acctctgcgt gaaggggcgc
     2881 tacgccttcg acttcgtacg ctcagccgac cgcgtcaccg agccgatgat ccgcaggggc
     2941 ggcgtgtggg agcgcgtctc gtgggcggag gccgtcgcct tcgccgccga aggcttgcgc
     3001 cgagttgtcg aacgacacgg ccccgacagc gtcggtgtcc tcggttcggc gcgcgcgacg
     3061 aacgaggaga actatcttac gcagaagttc gcacgcgtcg cgctcggcac gaacaacgtg
     3121 gactgctgcg cgcgcgtctg ccacacgccg accgccgccg cgatgaagct gatgctcggc
     3181 acgggcgcgg ccaccaactc gttcgacgac atcgagcgcg cccgcgccat cctcgtctgc
     3241 ggggctaacc cgaccgagag ccacccggtc gtgggagcgc gtatcaaaca ggctgcactc
     3301 cgcggcgcga agctcgtcgt catcgacccg cgcgggatcg agcttgcgcg ctacgccgga
     3361 cagcacctcg cgctccgacc gggtacgaac gtgccgctgc tgaatgcaat ggcgtacacc
     3421 atcatcgaag agaaactcta cgacgaagag tttatgcggg agcgcgtcaa cgggttggaa
     3481 gagttccgcg agttcatccg aacttattcg cccgaggcgg tcgcggccac ctgcggggtg
     3541 gaagccaacg tcatccgcga gaccgcgagg gtctacgcga ccgcgaagcc ggccatgtgc
     3601 ttccacggcc tcggcgtcac cgagcacacg caaggcacgg agggcgtgat gtgtctcgtc
     3661 aacctcgcgc tcctgacggg caacctcggt cagcccggct cgggcatcaa ccctctgcgc
     3721 gggcagaaca acgtgcaggg cgcggcgcac atggggtgcg accccggcac gctcaccggc
     3781 tccgtcccgc tcgccgaagg taaagactct ttcgagagag tgtggcgtgc ccccgtccca
     3841 atgcaaagag ggatgaacct gcttgagatg atggacgcgg cgggtcgggg caatctcaga
     3901 gctttgtggg ccatcggcta cgacatactt ctgaccaacg ccgacgcgca cgcgactgaa
     3961 cgcgcgctgc gctcactcga cctgctcgtc gttcaggact tgttcatgag cgagacggcg
     4021 aaggagttcg gcacggtctt cttcccggcg gcctcttcgt tcgagaagga tggaacgttc
     4081 atgaacgccg agcggcgcgt ccagcgcgtc agaaaggccg tcgagccgcc cggccattcg
     4141 aagcccgact gggaaatcat ctgcctgctc gcgcgcgcga tgggcaaggg cgatctcttc
     4201 ggttacgcgt cgcccgaaga ggtgtgggaa gaggttcgcg ccgtgtggcc gcagggccgc
     4261 gggatcacgt acgggcgtat cgaacgcggg gggctgcaat ggccctgccc cgcggaagat
     4321 catccgggca cacaggtgat tcacacggag tccttcccca tggggcagcg cgccgcgctg
     4381 cgccgcgtac ccttccgccc gacgcccgaa accacggacg gcgaataccc gttcctgctc
     4441 atcaccggca gaactctcta tccgttcaac gcgggcacga tgaccatgcg cacgcctaac
     4501 gcgtcgctcc gccccgccga caccctggat atgcatccgg acgacgcccg gcggctggcc
     4561 ttgcgcaagg gcgagcgcgt gcgcgtgcgg agccgttacg gcgaggccgt cctccccatc
     4621 aatctcacca acgcggtcaa gcgcggcgaa c
//
LOCUS       DBNGOH010001082         3277 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4929018, whole genome shotgun sequence.
ACCESSION   DBNGOH010001082 DBNGOH010000000
VERSION     DBNGOH010001082.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3277)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3277)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3277
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4929018"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2452)
                     /locus_tag="AC159P_21160"
     CDS             complement(<1..2452)
                     /locus_tag="AC159P_21160"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014026.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 protein"
                     /protein_id="HIR3698012.1"
                     /translation="MKARTNPVACSKVARRVAARALLSLVCVLLAAPPIRTGAQTSAP
                     PYKDPSLPIEKRVDDLVSRMTLEEKISQMMNGAAAIERLGVPEYEWWNEALHGVARAG
                     IATVFPQAIGLAATWHPELIREVADATSTEARAKHHEFVRRGERGRYKGLTFWSPNIN
                     IFRDPRWGRGQETFGEDPYLTARIGVAFVRGLQGDDPKYLKVAATAKHFAVHSGPEPE
                     RHSFDAKADERDMRETYLPAFRALIVEGRAEGVMCAYNRTNGEPACANKKLYDILRRE
                     WSFGGHVVSDCAAIEDIHKHHRFTKTEAEASAVAVRAGTDLTCGREYRSLGQAVKDGL
                     ITEAEIDVALKRLMRTRFRLGMFDPPEMVEYAGTPYSANDSAAHRALSLRAARESVVL
                     LKNENRTLPLSKEIRTIAVIGPNADAPDVLLGNYNGQPSKSVTPLQGIGAKVSSAMKV
                     LYSLGVYPTGALVVPVAPSALTGGDGARPAGLKGEYFDNKEWKGEPALVRTDADVNFN
                     WGSISPAPRIKDDNFSVRWTGTLTAPESGKYLLGFAGNGGARLVLDGKTVVEDFANRR
                     TRTLTKEVELEAGRRYDVRVEYQENANHYASARLLWAPPSGGRMLREDALAKAREADA
                     VVMVMGINPAVEGEEMDVKAEGFRGGDRTDISLPKPQEELIREVHALGKPVVLVLMGG
                     SALAVNWADEHVPAIVEAWYPGQEGGTALADVLFGDYNPAGRLPVTFYKSVAQLPPFE
                     EYGMAGRTYRYFKGEPLYPFGHGLSYTRFKYDKLKLGASRVKAGDGLRLSVDVQNVGD
                     RAGDEVVQLYLTDVAASVP"
     gene            complement(2515..3270)
                     /locus_tag="AC159P_21165"
     CDS             complement(2515..3270)
                     /locus_tag="AC159P_21165"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3698013.1"
                     /translation="MTGRVAVVVGGTSGLGRAIAVGLARAGAKTVPAGRRGELVREVC
                     EEITRGGGKAFARTADVADRDSVNTLRDAAIERFGGVDILVNAAGRTARRPTNEVSEE
                     EWAAIMDTNLTGMLRACQAFYGPLRASGRGRVINVASLSSYVAFHEVAAYGASKAGVT
                     ALTRSLGCEWARDSINVNAIAPGVFRTSLNAEFLDDTERGRELLMRTPMRRFGRPEEV
                     VGAAVFLASDASSFITGQTIVIDGGMLVSGVNT"
BASE COUNT          479 a         1169 c         1077 g          552 t
ORIGIN      
        1 ccggcaccga ggcggccacg tctgtcaggt acaactgcac cacttcatcg ccggcccggt
       61 cgcccacgtt ctgcacgtcc acgctgagac gcagcccgtc gccggccttg accctgctcg
      121 cgccgagctt caacttgtcg tacttgaacc gcgtgtagct cagcccgtgg ccgaacgggt
      181 agagcggctc gcctttgaag tagcgatagg tgcggcccgc catgccgtac tcttcgaacg
      241 gcggcaactg cgcgacggat ttgtagaacg tgacgggcag tcggcccgcc gggttgtagt
      301 cgccgaacag cacgtccgcc agcgccgtgc cgccttcctg ccccggatac cacgcctcga
      361 cgatggccgg gacgtgctcg tcggcccagt tgaccgcgag cgcgctgccg cccatcagca
      421 ccagcacgac cggcttgccg agcgcgtgga cttccctgat cagctcttcc tgcggcttcg
      481 gcagcgagat gtccgtgcgg tcgccgccgc gaaagccctc ggccttcacg tccatctctt
      541 cgccctcgac cgcggggttg atgcccatga ccatgacgac cgcatccgct tcgcgcgcct
      601 tcgcgagcgc gtcttcgcgc agcatgcgcc cgccggaagg cggcgcccac aacagccgcg
      661 ccgaagcgta atggttggcg ttctcctgat actcgacgcg cacgtcgtag cggcggcccg
      721 cttccagctc cacctccttc gtgagcgtgc gcgtccggcg gttggcgaag tcttcgacga
      781 cggtcttgcc gtcgagcacg agccgcgcgc ccccgttgcc cgcgaagccc agcaggtact
      841 tgcccgactc gggcgccgtg agcgtgcccg tccagcggac ggagaagtta tcgtccttga
      901 tccgcggcgc cgggctgatc gagccccagt tgaaattcac atccgcgtcc gtccgcacca
      961 gcgccggctc gcccttccat tctttgttgt cgaagtactc gcccttcaaa ccggcgggcc
     1021 gcgcgccgtc gccgccggtc agggccgacg gcgcgacggg aacgaccagc gcgccggtcg
     1081 ggtagacgcc gagggagtag agcaccttca tcgccgaaga gaccttcgcg ccgatgccct
     1141 gaagcggcgt gaccgacttc gacggctggc cgttgtagtt gcccaagagc acgtcgggcg
     1201 cgtccgcgtt cgggccgatg acggcgatgg tcctgatctc cttcgagagc ggcagtgtgc
     1261 ggttctcgtt cttcagcagc acgaccgatt cgcgcgccgc ccttaaagag agcgcgcggt
     1321 gcgccgccga gtcgttcgcc gagtatgggg tgcccgcgta ctcgaccatc tccggcgggt
     1381 cgaacatgcc gagccggaag cgcgtccgca tcaggcgctt gagcgccacg tcgatctcgg
     1441 cctcggtgat gagtccgtcc ttcaccgcct gcccgagcga gcggtactcc ctgccgcagg
     1501 tcaagtccgt gcccgccctg acggcgaccg cggaggcttc ggcctcggtc ttcgtgaagc
     1561 ggtggtgctt gtgtatgtct tcgatcgccg cgcagtccga gacgacgtgg ccgccgaacg
     1621 accactcgcg ccgcaggatg tcgtacagct tcttgttggc gcacgccggc tcgccgttcg
     1681 tccggttgta ggcgcacatc acgccctccg cgcggccttc gacgatcagc gcgcggaacg
     1741 cgggcagata cgtctcgcgc atgtctcttt catcggcctt cgcgtcgaag ctgtgccgct
     1801 ccggctccgg gccgctgtgc accgcgaagt gcttggccgt cgccgccacc ttcaggtatt
     1861 tggggtcgtc gccctgaagc ccacggacga aggcgacgcc gatgcgcgcg gtcaggtacg
     1921 ggtcttcgcc gaaggtctcc tgcccgcggc cccagcgcgg gtcgcggaag atgttgatgt
     1981 tgggcgacca gaaggtgaga cccttgtagc gcccgcgctc gccgcgccgg acgaactcat
     2041 gatgcttggc gcgcgcctcg gtcgaggtcg cgtcggcgac ctcgcggatg agttccgggt
     2101 gccacgtcgc ggccagcccg atggcctgcg ggaagacggt cgcgatcccg gcgcgggcga
     2161 cgccgtgcag cgcctcgttc caccactcgt actcgggcac gcccagccgc tcgatggccg
     2221 ccgcgccgtt catcatctgc gagattttct cttcgagcgt catccgcgag accagatcgt
     2281 caacgcgctt ctcgatcggc agagacgggt ctttataagg aggcgcggac gtttgcgcgc
     2341 cggtgcgaat cggcggcgcg gcgagcagca cgcagacgag cgacagcagc gcgcgcgcgg
     2401 cgacgcgacg agctaccttc gaacaagcga cagggtttgt gcgagctttc atggcatctt
     2461 ctgagcggtt ttcaattgaa tgtgcaacgc ggaatttcga tcgaacacac gatatcaggt
     2521 gttgacgccg gagacgagca tcccgccgtc gatgacgatg gtctggccgg tgatgaaaga
     2581 ggaggcgtcc gaagcgagga agaccgccgc gcccacgacc tcttcggggc gaccgaagcg
     2641 gcgcatcggc gtcctcatca gcagctcgcg cccgcgctcg gtgtcgtcga ggaactcggc
     2701 gttgagcgac gtgcggaata ctccgggcgc gatggcgttg acgttgatcg agtcgcgcgc
     2761 ccactcgcaa ccgaggctgc gcgtcagcgc cgtcacgccg gccttcgagg cgccgtaggc
     2821 cgcgacttcg tggaaggcga cgtacgaaga gagcgaggcg acgttgatga cgcgcccgcg
     2881 tccgctcgcc cgcagaggcc cgtagaacgc ctgacacgcg cgcagcatcc ccgtcaggtt
     2941 cgtgtccatg atcgcggccc actcctcttc gctcacttcg ttggtgggcc tgcgggcggt
     3001 gcgccccgcc gcgttgacga ggatgtccac gccgccgaat cgttcgatcg ccgcgtcgcg
     3061 cagcgtgttg acggagtcgc ggtcggcgac atccgccgtc cgcgcgaacg ccttgccccc
     3121 gccccgcgtg atctcctcgc agacctcccg caccagttcc ccgcgccgcc ccgcggggac
     3181 ggttttggcg ccggcccgcg ccagcccgac cgcgatcgcc cgccccagcc ccgaagtccc
     3241 gccgacgacg acggccaccc gcccggtcag ctcggtg
//
LOCUS       DBNGOH010001083         2511 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4379629, whole genome shotgun sequence.
ACCESSION   DBNGOH010001083 DBNGOH010000000
VERSION     DBNGOH010001083.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2511)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2511)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2511
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4379629"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(273..1355)
                     /locus_tag="AC159P_21170"
     CDS             complement(273..1355)
                     /locus_tag="AC159P_21170"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016876135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3698014.1"
                     /translation="MSEQLAEQSVKSKAEAFAEKMLGTINGAGLALMTSIGHRTGLFD
                     TMAALAPADAARVAEAAGLNERYVREWLGAMTTGGVVEYDPAQGTYHLPPEHAAFLTR
                     AASPNNLAVTTQFVAVLGAVEGRIVECFRNGGGVPYSAFERFHETMAEESEQTVVAGL
                     FDSILPLAPGLDVALEAGIDVLDVGCGSGRALITLARAFPKSRFTGFDLSEDAVSNAR
                     REAVRQGLTNVRFEARDVTVIGEEARYDLVTAFDAIHDQARPALVLRGIADALRPGGT
                     FLMQDIRGSRHVERNLDHPVAPFLYTISAMHCMTVSLASGGDGLGTMWGVETAVEMLT
                     EAGLRDIEVRQLPHDFMNNFYLGRKF"
     gene            1538..>2511
                     /locus_tag="AC159P_21175"
     CDS             1538..>2511
                     /locus_tag="AC159P_21175"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009769997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR3698015.1"
                     /translation="MRTPPRSYEFGRFRVDAEERVLLYDGSEVALTQKAFEVLLALVE
                     RGGHVVSKEELIRKVWSEQFVDEGNLTQNVYMLRKILGETEGGQQYIETVPRRGYRFK
                     PRVEVSEQDDAGAAQAATRAGAGVGEGKAAAPARTTSAAEPFVQPKTSYARSGEVNIA
                     YQVVGQGPLDVVFVMGWVSHLEYFWQEASFSRFLRRLASFSRLILFDKRGTGLSDRVP
                     LHELPTLEQRMDDVRCVMEAVGSARAALVGVSEGGPMSALFAATYPERTAALVMIGTY
                     AKRVRAEDYPWAPTAEDREHFFAEMYEHWGGPVGLEERAPSKANDPAFR"
BASE COUNT          393 a          829 c          883 g          406 t
ORIGIN      
        1 ggggtcgagc aggggcagct cgcggctgaa cgagaacgaa gattcagtgt agccgtggag
       61 catgatgtca gggcggcccg aagggtctcc ctgctcgctg taacgcagcc gcacgcccgt
      121 cggcacttgc atgtcggcga cgcggcgtgg cggtgtggcg atgtttttca tctctcgaag
      181 tctccttctt cgtccgccca ctcgcccatc acgagtgggc ggacgttcgt gttcatcccc
      241 cttgttcgtc gaatcttgcc ggccactgtg cgtcagaact tccgcccgag gtagaagttg
      301 ttcatgaagt cgtgcgggag ctgacggact tcgatgtcgc ggaggcccgc ctctgtgagc
      361 atctcgaccg ccgtctcgac gccccacatc gtcccgaggc cgtcgccgcc cgaggcgagc
      421 gagaccgtca tgcagtgcat cgcggagatg gtgtagagga acggcgcgac cgggtggtcg
      481 aggttccgct cgacgtggcg cgagccgcgg atgtcctgca tcaggaacgt gccgccgggc
      541 cggagcgcat cggcgatgcc gcgcaggacg agcgcgggcc gcgcctgatc gtggatggcg
      601 tcgaaggccg tgaccagatc gtaccgcgcc tcttcgccga tgacggtcac gtcgcgcgcc
      661 tcgaagcgca cgttcgtcag gccctgacgc acggcctcgc gccgcgcgtt cgagacggca
      721 tcctcagaga ggtcgaagcc ggtgaaccgg ctcttcggga aggcgcgcgc cagtgtgatc
      781 agcgcgcgac cgctgccgca gcccacgtcg agcacgtcga tgccggcttc gagggccacg
      841 tcgaggccgg gcgcgagcgg caggatcgag tcgaacaggc ccgcgacgac ggtctgctcg
      901 ctctcttcgg ccatcgtctc gtggaagcgc tcgaacgccg agtaggggac gccgccgccg
      961 ttgcggaagc attcgacgat gcggccttcg accgcgccga ggacggcgac gaactgcgtc
     1021 gtcacggcca gattgttcgg cgaggccgcg cgcgtcagga aggccgcgtg ctcgggcgga
     1081 aggtgatagg tgccctgcgc ggggtcgtat tccacgacgc cgcccgtcgt catcgcgccg
     1141 agccattcgc ggacgtagcg ctcgttgagt ccggcggcct ctgcgacgcg cgccgcatcg
     1201 gcaggcgcga gcgccgccat cgtgtcgaag agaccggtgc ggtggccgat gctcgtcatc
     1261 agcgcgaggc cggcgccgtt gatcgtgccg agcatcttct cggcgaacgc ctcggctttg
     1321 gatttcacag actgttccgc caactgttca gacatcgtct tctccttagt cgccgcgcac
     1381 actcaggcgc gcggtcttgc tgtataatcg gcccgcgccg gggcggcgaa agcacactcg
     1441 cgccggcggg ccgctattcc gttacgcgcg ggcggcggcc ccgaaggttt cagacgccgc
     1501 tcaaatagtt ctcaaatagt tctcaaacca gcttgcgatg cgcacgcccc cacgctccta
     1561 cgagttcgga cgcttccgcg tggacgccga agagcgcgtg ctcctctacg acggcagcga
     1621 agtcgcgctc acgcagaagg cgttcgaagt gctcctggcg ctcgtcgagc gcggcggcca
     1681 cgtcgtcagt aaagaagagc tgatccggaa ggtctggtcg gagcagttcg tggacgaagg
     1741 caacctcacg cagaacgtct acatgctgcg caagattctg ggcgagacag agggcgggca
     1801 gcagtacatc gaaaccgtcc cgcgccgcgg ctacaggttc aagccgcgcg tcgaggtgtc
     1861 ggagcaggac gacgcggggg cggcgcaggc ggcgacgcgc gcgggcgcgg gcgtcgggga
     1921 agggaaggcg gcggcgcccg cccgcacgac ttccgccgcg gagcctttcg tccagccgaa
     1981 gacctcttac gcgcgcagcg gcgaggtgaa catcgcgtat caggtcgtcg ggcaggggcc
     2041 gctcgacgtg gtcttcgtga tgggctgggt ctcgcacctc gaatacttct ggcaggaggc
     2101 gtcattctca aggttcctgc ggcgcctcgc ctccttctcg cggctcatcc tgttcgacaa
     2161 gcgcgggacg gggctttccg accgggtgcc gctgcacgag ctgccgacgc tcgaacagcg
     2221 gatggacgac gtgcgctgcg tgatggaggc ggtcggctcc gcgcgcgcgg cgctcgtcgg
     2281 tgtctcggag ggagggccga tgtccgcgct cttcgcggcc acctacccgg agcgcacggc
     2341 ggcgctcgtg atgatcggca cctacgccaa acgcgtccgc gccgaagact acccgtgggc
     2401 gccgaccgcc gaagaccgcg agcacttctt cgcggagatg tacgaacact ggggcggccc
     2461 cgtcgggctg gaagagcgcg cgccgagcaa ggccaacgac cccgctttcc g
//
LOCUS       DBNGOH010001084         2390 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2913585, whole genome shotgun sequence.
ACCESSION   DBNGOH010001084 DBNGOH010000000
VERSION     DBNGOH010001084.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2390)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2390)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2390
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2913585"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1527)
                     /locus_tag="AC159P_21180"
     CDS             complement(<1..1527)
                     /locus_tag="AC159P_21180"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012961.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
                     /protein_id="HIR3698016.1"
                     /translation="MTMLTRRCYQLILVAAALLALAPRALTVARTNESRIRLDTVGYL
                     PRHEKRASVAARFGEFEVVREPGGTVAFGGRAAGPLRNEDTGEELYFADFSALEEPGT
                     YFLDIPGVGRSAPFRVGRDVYDAPFRAAFAAMYLWRCGAAVRAARDGVTYSHAACHLE
                     DARLDFVGGGPARRDAAGGWHDAGDYNKYVVNAGVTVGIMLRAWEEFGARIARVRFDD
                     LPHGEARLPVYLQEIKWETDWLLKMQAADGSVYHKVSTKDFGPTIPPEDEKTARYFAP
                     WSSAATADFAAMLAAAARAFRPYDDEYSRRCLQASRRAYDFLRARPANQRADLKGFTT
                     GAYQTDDADDRLWAAAELWETTGERVYLADFERRARATPEKFDTDWGWSNVKNLGMLT
                     YLSSGRGGRDARLVGEVRRALVEVADLLVRAADEHGYARPLGTKYYWGANGGVANTTL
                     LLQAARRFAPKPAYAETALDAIHHLFGRNYYGRSFVTGLGANPPRRPHDRRSMGRPDA
                     P"
     gene            1735..2376
                     /locus_tag="AC159P_21185"
     CDS             1735..2376
                     /locus_tag="AC159P_21185"
                     /inference="COORDINATES: protein motif:HMM:NF012692.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HIR3698017.1"
                     /translation="MKRINLKKASVARADTIRTINRQIILNYVRERAPISRAEIAHET
                     ALQRSTVSLIVEELKVGGMVEEIEGESTGGRPPLLLRLRASGPVAVGVDLGATRTVVA
                     TSDLAGRVLEQEEFETDPDAARTLRRVTACVAGLVRKNRGLIEGVGVSLPGLVDYETG
                     SALFIPSFKWRDWPISREVGAATAGRGRGCTRPPPASTPPRSASTHGRFCRRG"
BASE COUNT          362 a          808 c          871 g          349 t
ORIGIN      
        1 gggcgcgtcg ggccgcccca tcgagcgccg gtcgtgcggg cggcgcggcg ggttcgcgcc
       61 gaggccggtg acgaacgagc gcccgtagta gttgcgcccg aagaggtgat gaatcgcgtc
      121 gagcgccgtc tcggcgtagg ccggcttcgg cgcgaagcgg cgggcggctt gcagcagcag
      181 ggtcgtgttg gcgacgccgc cgttcgcgcc ccagtagtac ttcgtgccga gcgggcgcgc
      241 gtagccgtgc tcgtccgccg cgcggacgag caggtcggcc acttcgacga gcgcgcggcg
      301 cacctcgccg acgagccggg cgtcgcgccc cccgcgcccg gacgacaggt aagtgagcat
      361 gccgaggttc ttgacgttcg accaccccca gtccgtgtcg aacttctcgg gcgtggcgcg
      421 cgcccgccgc tcgaagtccg cgaggtagac gcgctcgccc gtcgtctccc acaactcggc
      481 ggcggcccag aggcggtcgt ccgcgtcgtc ggtctggtac gcgcccgtgg tgaagccttt
      541 caggtcagcg cgctgattgg cggggcgcgc gcgcaggaag tcgtaagcgc ggcgcgacgc
      601 ttgaagacag cggcgcgagt attcatcgtc gtaggggcgg aaggcgcgcg cggcggcggc
      661 gagcatggcc gcgaaatcgg cggtggccgc gctgctccac ggcgcgaagt agcgcgcggt
      721 cttctcgtct tcgggcggga tggtcgggcc gaaatctttg gtcgagacct tgtggtagac
      781 cgagccgtcc gcggcctgca tcttgagcag ccagtccgtc tcccacttta tctcttgaag
      841 gtagacgggc aggcgcgcct cgccgtgcgg caggtcgtcg aacctgaccc gcgcgatccg
      901 cgcgccgaac tcctcccacg cgcggagcat gatgcccact gtgacgccgg cgttgacgac
      961 gtacttgtta tagtcgcccg cgtcgtgcca gccgcccgcg gcgtcgcgcc gcgcggggcc
     1021 cccgccgacg aagtcgagcc gcgcgtcttc gaggtggcag gcggcatgtg agtaggtgac
     1081 gccgtcgcgc gcggcgcgca cggcagcgcc gcaccgccag aggtacatgg ccgcgaaggc
     1141 ggcgcggaag ggcgcgtcgt acacgtcgcg cccgacgcgg aagggggccg agcggcccac
     1201 gccgggaata tccagaaagt acgtgcccgg ctcttcgagc gcggagaagt ccgcgaagta
     1261 aagctcttcg cccgtgtcct cgttgcggag tggccccgcc gccctcccgc cgaaggcaac
     1321 ggtgccgccc ggctcgcgca cgacttcgaa ctcgccgaag cgcgccgcga cggacgcgcg
     1381 cttctcgtgg cgcggtaggt agccgacggt gtcgagcctg atgcgactct cattagtccg
     1441 tgcgaccgtt aaggcgcgcg gcgcgagagc caacagcgcg gcggcgacga ggatgagttg
     1501 ataacatctg cgtgtcaaca tcgtcatcaa aaagtcaggc gcgcggcagg cgtcgagtca
     1561 tgcgcaggcg cgtacctgtc gcggcgggat aagagagcaa taaagtagta tcatgccagt
     1621 caagtgacag tgtgtccgta gcgggcctcg acgttgtgcc gtctttaatt cccgtgaccc
     1681 gaccccggtc gcggctgtag agcagggccg gcagcggccc tgcggagccg agccgtgaag
     1741 cgaatcaacc tgaagaaggc cagcgtcgca cgcgccgaca ccatccgcac catcaaccgc
     1801 cagatcatcc tcaactacgt ccgcgagcgc gcccccatct cgcgcgccga gatcgcccac
     1861 gagacggcct tgcagcgctc gaccgtctca ctcatcgtcg aggagctgaa ggtcggcggg
     1921 atggtcgagg agatcgaggg cgagtccacg ggcgggcggc ccccgctgct gctgcgcctg
     1981 cgcgcctccg gccccgtggc cgtgggcgtt gacctcggcg cgacgcgcac cgtcgtggcg
     2041 accagcgatc tggcgggccg cgtgctggag caggaggagt tcgagaccga cccggacgcc
     2101 gcgcggacgc tccggcgcgt gaccgcatgc gtcgccgggc tggtcagaaa aaatcgcggg
     2161 ctgatcgaag gggtcggggt gagcctgccc ggtctggtgg attacgagac gggcagcgcg
     2221 ctcttcatcc cgtccttcaa gtggcgcgac tggccgatca gccgcgaggt gggcgcggcg
     2281 acggcaggcc gcggcagagg ctgtactcgg ccaccgcctg cttcaactcc tcctcgatca
     2341 gcgtccacgc acggacgatt ctgccgccga ggatgacggc gtccgggctg
//
LOCUS       DBNGOH010001085         6492 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2363688, whole genome shotgun sequence.
ACCESSION   DBNGOH010001085 DBNGOH010000000
VERSION     DBNGOH010001085.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6492)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6492)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6492
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2363688"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2079
                     /locus_tag="AC159P_21190"
     CDS             <1..2079
                     /locus_tag="AC159P_21190"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024754.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HIR3698018.1"
                     /translation="RRDIEKLEEGYTRSFTIYDQDDTVRLVKGCIKDLGYDDQRLAPR
                     QTQSAISHAKNSGLDAEQYAARVESHDEKRAAIARVFRLYEERLVNNNALDFDDLLIK
                     AVRLLRKREDVRTQYNERFRYILVDEYQDTNSLQFALIRFLTEQQQNICVVGDESQSV
                     YRWRGADITNILNFEEHYPEATVIKLEQNYRSTQTILEAADAVIKHNTARKDKTLWTE
                     NPAGDMVRYYQAMDAEGEARFVAAKIDEHRRRESDLRAAVLYRTNSQSRVFEEALRRA
                     GLGYNIVGGYSFYERAEVRDIVSYLKLALNPHDSIALLRVINTPPRGLGKQTLDELDR
                     RAKDYGVSLWETIGIVTSQGENFSPRAVSALKAFQSIITNLVAVAQKEPASEVVKAAI
                     LDTGYSEALKTENSEEAEGRLENLQELVNAAVDYDKEEGGGLRDFIDAAALVSDSDQY
                     KAEAPVTLMTMHSAKGLEFPLVFVVGMEDGLFPHARSAGDQAEMEEERRLCYVAITRA
                     ERFLYVTHAMRRRVYGEELAAEPSQFLNELPLDLMEDLSRGNSWLSFTRSSSATEARH
                     TARVLRGDVPQKPQKTYGGKTYDSVDSIAEFFRQRGDKFKSPTPPTPQRTAPPPAVKR
                     QAAAAAGDSSSSSSGAGFVPGTHVRHPKYGRGLVLRREGTGDAAKLTVSFPGFGQKKL
                     IEKYAGLEKA"
     gene            2278..5877
                     /locus_tag="AC159P_21195"
     CDS             2278..5877
                     /locus_tag="AC159P_21195"
                     /inference="COORDINATES: protein motif:HMM:NF024753.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB
                     family protein"
                     /protein_id="HIR3698019.1"
                     /translation="MRKSLPLVASVLLAVLTAQFPAPLAATSARQTGRVRGKARPRAA
                     TNATVAADGAERDATAARQVTAGPRLVVQLGQRTSLYGFAVFSRDGRLVATGGGGDVV
                     VWDVATGREVRRLVGNEGDPGAQDLDGLTGGAFSPDGRLMAVAAGPNLRLWNVETGRL
                     VWRRENAANAFLVQEESQPPVAFTADGRQIVVRGDAARLVWDAATGKLLSRAGLTKKG
                     AVDGVTEDEMLARRVTSPDGALVVDAKAGVLNVTEKASGRRLGSVRFREAGAGDDEGD
                     GLLAFALSPDNRLLVTTHKVDDETESVRVWDVRTGEPVLALAGRQPGDAGALRFTADG
                     GLLAVSADGEIVRARDLASGHVVEWKADDVPSWLEVSADGRFVVTGAGRVGEVWDART
                     GARLARVQADLEEDAADFRWDFAGFTPDETVTFRKESTMNDDTGRVFRLSMRDWKEKQ
                     VNGLGTMPLGTGEGSEKGFVLNGDTRAAAWEIVESGADDKPNRHLVTVWNADPKVKNK
                     TFEVDRDEAYGDASGLALSPDGRRLVVVTANAGGNLAQSLVKVWDVEAKRALFKLNAP
                     GFRVGSTVAWSPDGRLIATGADDGTVVLWDAASGKESRRIKGRLASVGKVEFSRDSRL
                     LMASSGEGVRLLMDAATGAEVCRLVTFADGGWIVVDPQGRFDADDLADMRGAHWVAPD
                     DPFKPLPLEIFMRDYYEPRLLARLLAGERFAPVRALAELNRRQPLVRLASVEQQKERP
                     ELVNVTIEVEKGGDGSQQGARGVEREAGVYDLRLFRDGQLVGHAPEGAGGELKTDAAT
                     GRATITFKDIRLPRRQNLKEVEFTAYAFNADRVKSETARRTLKLDAAPAPVKGRAYVV
                     SVGVNAYENAKWDLRFAANDARRLQEVVSARVAGSGEYAEIVRVPLVSDYESKEGKKA
                     APRVVTEASATKANFRGVLDLLAGREVDAETRARLPNVEQLREAAPEDLVIISFSSHG
                     YADERGNFYLLPYDTGAGAEMRDVLARAISSEELSLWLREVDAGELVLVVDACHSAAS
                     VQGPGFKPGPMGSRGLGQLSYDKGMRVLTSTQADNVALETNLTEQGLLTYTLTREGLE
                     AARADFSPPDGQITVAEWLSYGVERVPTLFEEVGRRLAEMQAGRGAELSQESRTRVVV
                     FTPDGLAATPAAAPGGDGKGLREMTLSAFVNKTQRPTLFDYARGRRASVLSRK"
     gene            5998..6456
                     /locus_tag="AC159P_21200"
     CDS             5998..6456
                     /locus_tag="AC159P_21200"
                     /inference="COORDINATES: protein motif:HMM:NF026066.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HIR3698020.1"
                     /translation="MTNEEIARRFERLATLMTLRGDDQFRTRSYRNAAEVIETWPTPL
                     SRIAAEEGLKGLQTLPGVGKAISAKIVELLERGTFEAWERLTAETPETVLDLLRVEGV
                     GMKTAAALHQQFKISSLEDLRQFVEGGGLDLVDGVGERTAERIAASVRKL"
BASE COUNT         1126 a         2064 c         2346 g          956 t
ORIGIN      
        1 cgccgcgaca tcgaaaagct cgaagagggc tacacgcgct cgttcaccat ctacgatcag
       61 gacgacacgg tgcgcctcgt caagggctgc atcaaagacc tcggctacga cgaccagcgg
      121 ctcgcgcccc gccagacgca gagcgccatc agccacgcca agaacagcgg cctcgacgcc
      181 gaacagtacg ccgcgcgcgt cgagtcgcac gacgagaagc gcgccgccat cgcgcgcgtc
      241 ttccgcctct acgaggagcg tctggtcaac aacaacgcgc tcgacttcga cgacctgctc
      301 atcaaggccg tccggctcct gcgcaagcgc gaggacgtgc gtacacaata caacgagcgc
      361 ttccgctaca tcctcgtgga cgagtatcag gacacgaact ccttgcagtt cgcgctcatc
      421 cgttttttga cggagcagca gcagaacatc tgcgtcgtcg gcgacgaaag ccagagcgtc
      481 taccgctggc gcggcgccga catcacgaac atcctcaact tcgaagagca ctaccccgaa
      541 gcgaccgtca tcaagctcga acagaactac cgctcgacgc agaccatcct cgaagccgcg
      601 gacgccgtca tcaagcacaa cacggcgcgc aaggacaaga ccctctggac ggagaacccg
      661 gcgggcgaca tggttcgcta ctatcaggcg atggacgccg aaggcgaggc gcgcttcgtc
      721 gccgcgaaga tcgacgagca ccggcggcgc gagtcggatt tgcgcgccgc agtgctctac
      781 cgcaccaact cgcagtcgcg cgtgttcgaa gaagcactca ggcgcgcggg cctcggctac
      841 aacatcgtcg gcggctactc gttctacgag cgcgcggagg tgcgcgacat cgtctcgtat
      901 ctgaagctcg ccctgaaccc gcacgactcg atcgcgctcc tgcgcgtcat caacacgccg
      961 ccgcgcggcc tcggcaagca gacgctcgac gaactcgacc gccgcgcgaa ggactacggc
     1021 gtctctctgt gggagaccat cggcatcgtc acgagtcagg gcgagaactt cagcccgcgc
     1081 gccgtctccg ccttgaaggc gttccagtcg atcatcacta atctcgtcgc ggtcgcgcag
     1141 aaggaacccg cgagcgaggt ggtcaaggcc gcgattctcg acacgggtta ctccgaggcg
     1201 ctgaagacgg agaacagcga ggaggccgag ggccgtctgg agaacttgca ggagctggtc
     1261 aacgccgccg tcgattacga caaggaggag ggcggcgggc tgcgcgactt catcgacgcc
     1321 gcggcgctcg tctccgactc cgaccagtac aaggcggaag cgcccgtcac gctgatgacg
     1381 atgcactcgg ccaagggcct tgagttccct ctcgtcttcg tcgtcgggat ggaagatggt
     1441 ctgttcccgc acgcgcgctc ggcgggcgat caggcggaga tggaagagga gcgccgcctc
     1501 tgctacgtcg ccatcacgcg cgccgagcgt ttcctctacg tgacgcacgc gatgcgccgc
     1561 cgcgtctacg gcgaggagct ggccgcagag ccttcgcagt tcttgaacga gctgccgctc
     1621 gatctgatgg aagacctctc gcgcggcaac tcgtggctct cgttcacgcg cagctcttcg
     1681 gccaccgagg cgcgccacac ggccagagtc ctgcgcggcg atgtaccgca gaagccccag
     1741 aagacttacg gcggcaagac ctacgacagc gtcgattcca tcgccgagtt cttccgccag
     1801 cgcggcgaca agttcaagtc cccgacgccg ccgacgccgc aacgaaccgc cccgccgccc
     1861 gccgtcaaac gacaggcggc ggctgccgcg ggcgactcgt cttcctcgtc ttccggcgcg
     1921 ggcttcgtgc ccggcacgca cgtccgccac cccaagtacg gtcgcggcct cgtgctccgc
     1981 cgcgaaggca cgggcgacgc cgccaagctc accgtcagct tccccggctt cgggcagaag
     2041 aagctgatcg agaagtacgc cggtctggag aaggcgtaga gttcattaca aaactgccca
     2101 gcgcgttcga agactgtagg ggcagggcaa gtccgcggcc cgccgctgac gaatccatcc
     2161 gacgttgaat ccacggcacc gtagtgggcc gcggacttgc cctgccccta cagtcttgcc
     2221 acgcgaactt aaccttaacc gcctgctccg tgccgttcga catttacgaa aaccttgatg
     2281 agaaaatcgc tgcccctcgt cgcttccgtc ttgctcgccg tcttgacggc gcagttcccc
     2341 gcccctctcg ccgcaacgtc agctcgacaa accggacgcg tgcgcggcaa agccaggccg
     2401 cgagcggcga cgaacgcgac cgtcgcggcg gatggtgcgg agcgtgatgc gacggcggcg
     2461 cggcaggtca ctgcggggcc gcggctcgtg gtgcagctcg ggcagcgcac ttccctctac
     2521 gggttcgcgg tgttctcgcg cgacgggcgg cttgtggcga cgggcggcgg cggcgacgtg
     2581 gtggtgtggg acgtggcgac ggggcgcgag gtgcggcggc tcgtggggaa cgagggtgac
     2641 cccggtgcgc aagacctcga cgggctgacg ggcggcgcgt tctcgccgga cggcaggttg
     2701 atggcggtcg ccgcggggcc gaacctgcgg ctgtggaacg tcgagacggg acggctcgtg
     2761 tggcggcgcg agaatgcggc caacgcgttc ctcgtgcagg aagagtcgca gccgccggtc
     2821 gcgttcacgg ctgacggtcg gcagatcgtc gtgcgcggcg acgcggcgag gctcgtgtgg
     2881 gacgcggcga cgggaaaact tttgtcgcgc gcgggcctga cgaagaaggg tgccgtggac
     2941 ggcgtgacgg aagacgagat gctcgcgcgc cgcgtcactt cgccggacgg cgcgctcgtg
     3001 gtcgatgcga aggccggtgt cttgaatgtg acggagaagg cgtcgggcag acgactcggg
     3061 agcgtgcgtt tcagggaagc gggcgcgggc gacgatgaag gcgacggtct gctcgcgttc
     3121 gcgctgtcgc cggacaatcg tttgctggtc acgacccata aggttgacga cgagacggag
     3181 tcggttcgtg tgtgggacgt gcggacgggc gagccggtgc tcgcgctcgc gggcaggcag
     3241 ccgggcgacg cgggggcgct aaggttcact gcggacggcg ggctgctcgc ggtgtcggcg
     3301 gacggtgaga tcgtgagggc gcgtgacctc gcgagcggtc acgtggtcga gtggaaggcg
     3361 gacgatgtgc cttcgtggct cgaagtctcc gcggacggtc gcttcgtcgt gacgggcgcg
     3421 gggcgcgtcg gggaggtgtg ggacgcgcgg acgggggcgc gactggcgcg cgttcaggcg
     3481 gacttggagg aagatgccgc cgacttcagg tgggacttcg cgggcttcac gccggacgag
     3541 accgtgacgt tccgcaaaga gtctacgatg aacgacgaca cggggcgcgt gttccgtctg
     3601 agcatgaggg attggaagga gaagcaggtc aacggactcg gcacgatgcc gctcggcacc
     3661 ggcgagggca gcgagaaggg cttcgtcctg aacggcgaca cgcgcgcggc ggcgtgggag
     3721 atcgtcgaga gcggcgcgga cgacaagccg aacaggcatc tcgtgacggt gtggaacgca
     3781 gacccgaagg tgaagaacaa gacgttcgag gttgaccgcg acgaggcgta cggcgacgcg
     3841 tcgggtctcg cgctctcgcc ggacgggcgc aggctcgtcg tcgtcaccgc gaacgcgggc
     3901 ggcaacctcg cgcagtcgct cgtcaaggtg tgggacgtgg aggcgaagcg cgcgctgttc
     3961 aagttgaacg cgccgggctt ccgcgtcggc tcgacggtcg cgtggtcgcc cgacggtcgc
     4021 ctgatcgcca cgggcgccga cgacggcacg gtcgtgctgt gggacgcggc gagcgggaag
     4081 gagtcgagaa gaatcaaggg gcggctcgcg tcggtcggca aggtcgagtt ctcgcgcgac
     4141 agccgcctgt tgatggcctc gtcgggcgaa ggcgtgcggc tgctgatgga cgcggcgacg
     4201 ggcgcggagg tgtgccgcct cgtgacgttc gcggacggcg gctggatcgt cgtcgacccg
     4261 caggggcgct tcgacgcgga cgacctcgcc gacatgcgcg gcgcgcactg ggtcgcgccc
     4321 gacgatccct tcaagccgct gccgctcgaa atcttcatgc gcgattatta cgagccgcgg
     4381 ctgctcgcgc ggctgctcgc gggcgagagg ttcgcgccgg tgcgcgcgct cgcggagttg
     4441 aaccggcggc aaccgctcgt ccgcttagcc tccgtcgagc aacagaagga gcggcccgag
     4501 cttgtcaacg tgacgatcga agtggagaaa ggcggggacg ggtcgcagca gggcgcccga
     4561 ggcgtcgagc gcgaggcggg cgtctacgac ctgcgactgt tccgcgacgg gcagctcgtc
     4621 ggccacgcgc ccgaaggcgc gggcggcgag ttgaagaccg acgccgcgac gggccgcgcg
     4681 acgataacat tcaaggacat caggctgccg cgccgtcaga atttgaagga ggtcgagttc
     4741 acggcctatg cgttcaacgc agaccgcgtg aagagcgaga ccgcgcgccg cacgctgaag
     4801 ctcgacgccg cgcccgcgcc cgtgaagggc cgcgcttacg tcgtctcggt cggcgtcaac
     4861 gcgtacgaga acgcgaagtg ggacttgcgc ttcgcggcga acgacgcgcg gcgtttgcag
     4921 gaggtcgtct cggcgcgcgt cgccgggtcg ggcgagtatg cagagatcgt gcgcgtgccg
     4981 ctcgtctcgg attacgagtc gaaggagggg aagaaggcgg cgccgcgcgt cgtcaccgaa
     5041 gcgtcggcga cgaaggcgaa cttccgcggc gtgctcgacc tgctcgcggg ccgcgaggtg
     5101 gacgcggaga cgcgcgcgag gctcccgaac gtcgaacagt tgagagaggc ggcgccggaa
     5161 gatttggtca tcatctcttt ttcgagccac ggctacgcgg acgagcgcgg caacttctac
     5221 ctgctgccct acgacacggg cgcgggcgca gagatgcgcg acgtgctggc gcgcgcgatt
     5281 tcgagcgaag agctttcgtt gtggctgcgc gaagtggacg cgggcgaact cgtcctcgtg
     5341 gtggatgcgt gccactcggc ggcgagcgtg caggggccgg gcttcaagcc ggggccgatg
     5401 ggcagccgcg gcctcgggca gttgtcatac gacaagggga tgcgcgtcct gacttcgacg
     5461 caggccgaca acgtcgcgct cgaaacgaac ctcaccgagc aaggtcttct gacttacact
     5521 ttgacgcgcg aagggctgga agcggcgcgc gcggacttca gcccgcccga cggccagatc
     5581 acggtcgcgg agtggctgtc gtacggcgtc gagcgcgtgc ccactttgtt cgaggaggtc
     5641 gggcggcgcc tcgccgagat gcaggcgggc aggggcgccg agctgtcgca ggaaagccgg
     5701 acgagggtcg tcgtcttcac gccggacggc ctcgccgcga cgccagccgc cgcgccgggc
     5761 ggcgacggca aagggctgcg cgagatgact ctctccgcct tcgtcaacaa gacgcagcgc
     5821 cccacgctct tcgactacgc gcgcgggcgt cgcgcctccg tgttgtcgag gaaatagcgg
     5881 gcagcgggca gcgggcagca ggcagtaaaa gcatttcggg attcacgagt cgaggttaaa
     5941 atcctgaaat gcttttgctg cctgctgcct actgcctact gaccgctctt gaagcttatg
     6001 actaacgagg agatcgcgcg gaggttcgag cggctggcga cgctgatgac gctgcgcggc
     6061 gacgaccagt tccggacgcg ttcgtaccgc aacgccgcgg aggtgatcga gacgtggccg
     6121 acgccgctca gccgcatcgc cgccgaagag ggcttgaagg ggttgcagac cttgccgggc
     6181 gtgggcaagg ccatcagcgc gaagatcgtc gagctgcttg agcgcggcac gttcgaggcg
     6241 tgggagaggc tgacggcgga gacgcccgag accgtgctcg acctgctgcg cgtcgagggc
     6301 gtcggcatga agaccgccgc cgcgctccac cagcagttca aaatctcctc gctcgaagac
     6361 ctgcgccagt tcgtcgaggg cggcggcctc gacctcgtgg acggcgtcgg cgagcggacg
     6421 gcggagcgca tcgccgccag cgtcagaaag ctttaaggca gacgggccgg caagggggag
     6481 gggcgacggg ga
//
LOCUS       DBNGOH010001086         2541 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1447864, whole genome shotgun sequence.
ACCESSION   DBNGOH010001086 DBNGOH010000000
VERSION     DBNGOH010001086.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2541)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2541)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2541
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1447864"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..719
                     /gene="galT"
                     /locus_tag="AC159P_21205"
     CDS             <1..719
                     /gene="galT"
                     /locus_tag="AC159P_21205"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007914524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HIR3698021.1"
                     /translation="HDLTLSRMDDASVDAVLACFQEQYRALGARPGVAHVLMFENKGE
                     AVGVSNPHPHCQIYATNFVFKTIETEARASQRHLAETGRVLFQDILAAERLDGRRVIA
                     ENDSAIAFLPYFARYAYEVFVAPKETHPSIAALSPAEVRDFAAVLKTVLVKFDNLWRM
                     PFPYVMPLHQAPTDGGDYSGFHFHIEFHPPLRKPNLLKYLAGPEIGGGNFLSDTSPQE
                     KAAELRALPDVHYKHAPEGV"
     gene            719..1747
                     /locus_tag="AC159P_21210"
     CDS             719..1747
                     /locus_tag="AC159P_21210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007914525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HIR3698022.1"
                     /translation="MQDIRDLLAPIRELHEQIRAAVVAETERASTEELAAVVKDDEGD
                     TIFAVDRVSEELLVEFFEREVAPGSPLVLIAEGLEGGQIILPHGTLEADALWRVVVDP
                     IDGTRGLMYQKRSAWILTGVAPNRGLETSLQDIELAVQTEIPLVKQHLSDAAWAVRGE
                     GAQAERLNRLTGERAPLSLRPSRSATMLNGYAQIARFFPGARDVLAEIDEEIVRGALG
                     PAPEGKALCFEDQYTSSGGQLYELMTGHDRFTADLRALLGPVMEARGLPMSLCCHPYD
                     LCTELIARELGVVVTDARGGPLGATLAVEPDVAWAGYANEQLRARIEPLLRSALGGRG
                     LLDETQRG"
     gene            1754..>2541
                     /locus_tag="AC159P_21215"
     CDS             1754..>2541
                     /locus_tag="AC159P_21215"
                     /inference="COORDINATES: protein motif:HMM:NF012509.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GHMP family kinase ATP-binding protein"
                     /protein_id="HIR3698023.1"
                     /translation="MYRIAHGTTHDLADVSDFVGLLGALDQHPAPEVRGLFDPRADIY
                     VARAPGRLDVMGGIADYSGSLVLEMPIHEATLVALQRDAERRLQVVSLSEEPGRVLTF
                     EMPLADFDGDGEAMSYEAAREYFRRDPARHWAAYVAGVVLVLMREHGAEFREGARVLV
                     ASRVPGGKGVSSSAALEVATMQAVAAAFDVALDARDTALLCQRVENLVVGAPCGVMDQ
                     MTSVCGEEGRLLTLLCQPAELQGVVEIPEEVSFWGLDSGVRHAVT"
BASE COUNT          376 a          887 c          879 g          399 t
ORIGIN      
        1 ggcacgacct cacgctctct cgcatggacg atgcctccgt cgacgcggtg ctggcctgct
       61 tccaggagca gtaccgcgcg ctgggcgcgc gacccggagt cgcgcacgtg ctgatgttcg
      121 agaacaaggg cgaggcggtg ggcgtctcga acccgcaccc gcactgccag atttacgcca
      181 ccaacttcgt cttcaagacc atcgagaccg aggcgcgtgc ctcgcaacgt catctggcgg
      241 agacgggccg cgtcctcttt caggatatcc tcgcggccga gcggctggac gggcgccgcg
      301 tcatcgccga gaacgactcg gccatcgcct ttctgcccta cttcgcgcgc tacgcctacg
      361 aagtcttcgt cgcgccgaag gagacgcacc cttcaatcgc cgcgctctcg ccggcggagg
      421 tgcgggactt cgccgcggtg ctcaagaccg tcctcgtcaa gttcgacaac ctgtggcgga
      481 tgccctttcc ctacgtcatg cctctgcatc aggcgccgac cgacggcggc gactacagcg
      541 gcttccactt ccacatcgag ttccacccgc cgctccgcaa gccgaacctc ttgaagtacc
      601 tcgccggccc cgagatcggc ggcggcaact tcctctccga cacctcgccg caggagaagg
      661 ccgcggagct gcgcgcgctg cccgacgtac attacaaaca cgcgccggag ggcgtctgat
      721 gcaagacatc cgcgacctgc tcgcgccgat acgcgagctg cacgaacaga tccgcgcggc
      781 ggtcgtcgcg gagaccgagc gcgcctcgac ggaagagctg gccgcggttg tcaaggacga
      841 cgagggcgac acgatcttcg ccgtcgatag ggtgagcgag gagctgctcg tcgagttctt
      901 cgagcgcgag gtggcgcccg gctcgccgct cgtgctcatc gccgaaggtt tggaaggcgg
      961 tcaaatcatc ctgccgcacg gcacgctcga agcggacgcg ctgtggcgcg tcgtcgtcga
     1021 tccgatagac ggcacgcgcg gcctcatgta ccagaagcgc agcgcgtgga ttctgaccgg
     1081 ggtggcgccc aaccgcggcc tcgaaacgag cctccaggac atcgagctgg ccgtgcagac
     1141 cgagattccg ctcgtcaagc aacacctctc ggacgcggcg tgggccgtgc gcggcgaggg
     1201 cgcgcaggcc gagcgcctga atcgcctgac gggcgagcgc gcgccgctga gccttcgccc
     1261 ctcgcgctcg gcgacgatgc tgaacggcta cgcgcaaatc gcgcgcttct tccccggcgc
     1321 gcgcgacgtg ctcgccgaga tcgatgaaga gatcgtccgc ggcgcgcttg gccccgcgcc
     1381 cgagggcaag gcgctctgct tcgaagacca gtacacctct tcgggcggtc agctctacga
     1441 gctgatgacc ggccacgacc gcttcaccgc agacctgcgc gcgctgctcg gccccgtcat
     1501 ggaggcgcgc ggtctgccga tgagcctctg ctgtcaccct tacgatttgt gcacggaact
     1561 gatcgcgcgc gagctgggcg tcgtcgtcac ggacgcgcgg ggcggcccct tgggcgcgac
     1621 gctcgcggtc gagccggacg tggcgtgggc gggctacgcc aacgagcaac tgcgcgcgcg
     1681 tatcgagccg ctgctccggt cggcgctcgg cgggcgcggt ctgctggacg agacgcagcg
     1741 gggctgagtg tccatgtatc gcatcgccca cggcacgacg cacgacctcg cggatgtctc
     1801 cgacttcgtc gggctgctcg gcgcgctcga ccagcacccc gcgccggaag tgcgcgggct
     1861 gttcgacccg cgcgcggaca tctacgtcgc gcgcgcgccg ggccgcctcg acgtgatggg
     1921 cggcatcgcc gactactccg gctcgctcgt gctggagatg ccgattcacg aggcgacgct
     1981 cgtcgccttg cagcgcgacg ccgagcggag gctgcaagtc gtcagcctct cggaagagcc
     2041 gggccgcgtg ctcacgttcg agatgccgct cgcggacttc gacggcgacg gcgaggcgat
     2101 gagttacgag gccgcgcgcg aatacttccg ccgcgacccc gcgcgccact gggccgctta
     2161 cgtggccggc gtcgtgctgg tcttgatgcg cgagcacggc gccgagtttc gagaaggcgc
     2221 gcgcgttctc gtcgcttcgc gcgtgccggg cggcaagggc gtcagctcgt ccgccgcgct
     2281 cgaagtggcg acgatgcagg cggtggccgc cgccttcgac gtcgcgctcg acgcgcgcga
     2341 tacggcgctg ctgtgtcaaa gggtggagaa cctcgtggtc ggcgcgccgt gcggcgtgat
     2401 ggatcagatg acctcggtgt gcggcgagga gggacgcctc ctcacgctgc tctgccagcc
     2461 cgcggagttg cagggcgtgg tcgagattcc cgaggaggtc tcgttctggg gtctcgattc
     2521 gggcgtgcgc cacgcggtga c
//
LOCUS       DBNGOH010001087         4447 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3280911, whole genome shotgun sequence.
ACCESSION   DBNGOH010001087 DBNGOH010000000
VERSION     DBNGOH010001087.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4447)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4447)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4447
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3280911"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..411
                     /locus_tag="AC159P_21220"
     CDS             <1..411
                     /locus_tag="AC159P_21220"
                     /inference="COORDINATES: protein motif:HMM:NF013767.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_function: GO:0043424 - protein histidine kinase binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HIR3698024.1"
                     /translation="SFARAAADDAPFEEAAVDDVLDLRLIEHLRGFREQGDNGIIVQL
                     IDTFLQDINARRNSISEAYGSHDLERVRREAHALKGSAGFFGAVRLSRVCAELMDAAA
                     EGELPPAHQALKRLDDELLSITRKLEAWRCIHPA"
     gene            complement(493..2058)
                     /locus_tag="AC159P_21225"
     CDS             complement(493..2058)
                     /locus_tag="AC159P_21225"
                     /EC_number="3.5.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013377600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamate deiminase"
                     /protein_id="HIR3698025.1"
                     /translation="MTDSSDDRTAPKGDEGSGTLTTWVPDLLYVNGRFERGCALVCDD
                     AGRIVAVAHLNDDADRNDEARLESADAGGVPVTATRVVRLRGRALLPGMVNAHSHAFQ
                     RVIRGRTEHRSAVSSRDSFWTWREAMYEAAARLTPEDIYDASRMAFAEMALSGITAVG
                     EFHYLHRAPDGAEYEDPNLLAKQVVRAARDVGLRVALLRVFYARSGFRTPPNERQRRF
                     IEADPAVYVRHTEALMRDLRQADAAGGSANGGAWAGVAPHSVRAVMPEHLRAASAFAD
                     AHGLPLHMHVAEQPAEVAVCVAETGRTPVALLADEGVLSARFTGVHAVHITAEEARAL
                     ARAGATVCACPTTERNLGDGVVPADLLFAAGVAVSLGTDSHAQIDLLEDARELEYHLR
                     LVRVERNVLAPEANEAAWENEAAGRAANISALAARLFECATTSGARSIGAAGGALEPG
                     RPADFFTVDLDDPSIAGAGDEDLLPAVVFSLSRTAVREVAVGGRLVVEEGRHGAQEEI
                     VSRFAALQKKLRR"
     gene            complement(2083..4035)
                     /locus_tag="AC159P_21230"
     CDS             complement(2083..4035)
                     /locus_tag="AC159P_21230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HIR3698026.1"
                     /translation="MRSRFKGRLPALLAAAVLLSPVLPVAARQSAARGGGAMKTTDRN
                     APAAKALHKLFEDEWEWTMRENPTWASSLGDRRYNDRWEDVGLEAIEARHRHRQEALA
                     RLKAIDRARLSAADQLNHDLFRKDLETDIEEHGFRLYLLPVNQRGGIQTADELTELLR
                     FQTVKDYDDWVARLRQLPRYMEQTLALMREGVRAKKLWPHEVLKRVPAQIDKQLVARP
                     EDSPFYAPFKKFHDDVPAQERERLTREAREAVSQHVIPSYRQLKTYFDDEYLPASFPQ
                     VGVWQWPDGEAAYAFLARRYTTTDLTPEQVHEKGLSEVARIRAEMQKVMTEVGFKGTH
                     KEFFRKLRTSDEFYYQKPEELLEAYRAMSKRIDPHLVKVFKTIPRMPYGVVPIPDKIA
                     PDTTTAYYNQPAADGSRAGLYYVNLYKPEMRPKYEMMALSLHEAVPGHHLQIARAMEL
                     GEIPKFRRYGGYTAYVEGWGLYAESLGEDMGLYDDPYSKFGQLTYEMWRAVRLVVDTG
                     IHHMKWERRRAIDYFLDNAAKTEQDIVNEIDRYITMPGQALAYKIGELKLKKLRARSA
                     RELGPAFDVRDYHDVVLGSGAVPLDVLERNVDEWIASRKAARTDAQSLLDKKSGGGVS
                     SGAPRRKLTRGASFGERPPKKPPIKN"
     gene            4245..>4447
                     /locus_tag="AC159P_21235"
     CDS             4245..>4447
                     /locus_tag="AC159P_21235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3698027.1"
                     /translation="MSVSMNELKTYASYARRFGRRFMKERVRELRVPVAAPPVTDPWF
                     SALIDLTLGLTSEGAKPWVSYWRR"
BASE COUNT          662 a         1434 c         1582 g          769 t
ORIGIN      
        1 tccttcgcgc gggccgccgc cgacgacgca ccctttgaag aggccgcggt tgacgacgtg
       61 ctcgacttga ggctgatcga gcacctgcgc ggcttccgcg agcagggcga caacggcatc
      121 atcgtccaac tcatcgacac cttccttcag gacatcaacg cgcgccgcaa ctccatcagc
      181 gaggcttacg gctcacacga tctggagagg gtgcggcgcg aggcccatgc gctcaagggg
      241 agcgccggtt ttttcggggc cgttcgcctg tcgagggtct gcgccgaact catggacgcg
      301 gctgccgaag gcgagctgcc tcccgcacat caagcgttga aacgactcga cgacgagctg
      361 ctgagcatca cccgcaagct ggaagcgtgg aggtgcatcc accccgcata aataaagtga
      421 tgaggactga ggactgtgta aaggcaatcc gtcttttaca cagtcctcag tcctcagtcc
      481 tcatcactcg tttcacctcc ggagcttttt ttgcagcgcg gcgaagcggg agacgatctc
      541 ttcctgcgcg ccgtggcgtc cttcctcgac gacgaggcga ccgccgaccg ccacttcgcg
      601 gacggcggtg cgggagagcg agaagacgac ggcgggcagc aggtcttcgt cgcccgcgcc
      661 ggcaatcgag gggtcgtcca gatcgacggt gaagaagtcg gcgggccgtc cgggttcgag
      721 cgcgccgccc gcggcgccga tgctgcgcgc gccgctcgtc gtcgcgcact cgaacagccg
      781 cgccgcgagc gcggagatgt tcgcggcgcg ccccgccgcc tcgttctccc acgccgcctc
      841 attcgcttcg ggcgcgagca cgttgcgctc gacgcgcacg aggcgcaggt ggtactcaag
      901 ctcgcgcgcg tcttccagga ggtcgatctg cgcgtggctg tccgtgccga gcgagacggc
      961 gacgcccgcc gcgaagagca ggtcggcggg cacgacaccg tcgccgaggt tgcgctcggt
     1021 cgtcgggcac gcgcagaccg tcgcgcccgc gcgtgcgagc gcgcgcgcct cctccgccgt
     1081 gatgtggacg gcgtggacgc ccgtgaagcg cgcgctcaga acgccctcgt cggcgagcag
     1141 cgcgacgggc gtgcgccccg tctccgcgac gcagacggcg acctccgcgg gctgctccgc
     1201 gacgtgcatg tggagcggca ggccgtgcgc gtcggcgaag gcgctcgccg cgcgcaggtg
     1261 ttcgggcatg acggcgcgga cgctgtgcgg ggcgacgccg gcccacgcgc cgccgttggc
     1321 cgagccgccc gcggcatcgg cctgacggag gtcgcgcatc agggcttcgg tgtggcggac
     1381 gtagacggcg gggtctgctt cgatgaagcg gcgctgacgc tcgttcggtg gcgtgcgaaa
     1441 accggagcgc gcgtagaaga cgcgcagcag cgcgacgcgc agccccacgt cgcgggcggc
     1501 gcggacgacc tgcttcgcca gcaggttcgg gtcttcgtac tcggcgccgt cgggcgcgcg
     1561 gtgcaggtaa tgaaactcgc cgacggcggt tatccctgag agcgccatct cggcgaaggc
     1621 catgcgcgag gcgtcgtaga tgtcttcggg cgtgaggcga gcggcggctt cgtacatcgc
     1681 ctcgcgccac gtccagaacg agtcgcgcga agagactgcc gagcggtgct ccgtgcgccc
     1741 gcggatgacg cgctggaagg cgtgcgagtg cgcgttgacc atgccgggca gcagcgcgcg
     1801 gccccgcagg cggacgacgc gagtcgccgt gacgggaacg ccgcccgcgt ccgcgctctc
     1861 aagccgcgcc tcgtcgttgc ggtcggcgtc gtcgttgaga tgggcgacgg cgacgatcct
     1921 gcccgcgtcg tcgcacacga gcgcgcaccc gcgctcgaag cgcccattga cgtagagcaa
     1981 atcgggcacc cacgttgtca aggtgcccga cccttcgtcg cccttcggcg ccgtcctgtc
     2041 gtccgacgaa tcagtcatgc ctcgcttcgc ggcttcgggc gctcagttct tgatcggcgg
     2101 cttcttgggc ggcctctcgc cgaacgacgc gccgcgcgtg agcttcctcc gcggcgcgcc
     2161 ggacgacacg ccgccgcccg actttttatc cagtagcgac tgcgcgtccg tccgcgcggc
     2221 cttgcggctg gcgatccact cgtccacgtt tctttcgagc acgtcgagcg ggacggcgcc
     2281 ggagccgagc accacgtcgt ggtagtcgcg cacgtcgaac gcggggccga gttcgcgtgc
     2341 ggagcgcgcg cgcagcttct tgagcttcag ctcgccgatc ttgtaggcga gcgcctggcc
     2401 gggcatcgtg atgtagcggt cgatctcgtt gacgatgtcc tgctcggtct tggcggcgtt
     2461 gtcgaggaag tagtcgatgg cgcgccggcg ctcccacttc atgtggtgga tgccggtatc
     2521 gacgacgagg cgcacggcgc gccacatctc gtaggtgagc tggccgaact tggagtaggg
     2581 gtcgtcgtag aggcccatgt cttcgcctaa agattcggcg tagaggcccc agccctcgac
     2641 gtaggcggtg tatccgccgt agcggcggaa cttcggtatc tcgcccagct ccatcgcgcg
     2701 cgcgatctgc aagtggtggc cgggcacggc ctcgtgcaag ctgagcgcca tcatctcgta
     2761 cttcgggcgc atctcgggct tgtagaggtt gacgtagtag aggccggcgc gcgagccgtc
     2821 cgccgcgggc tggttgtagt aggcggtcgt cgtgtcgggc gcgatcttgt ctgggatggg
     2881 gacgacgccg tagggcatgc gcgggatggt cttgaagacc ttgacgaggt gcgggtcgat
     2941 gcgcttcgac atcgcgcggt aggcttccag aagctcctcc ggcttctggt agtagaactc
     3001 gtccgaggtg cggagcttcc tgaagaactc cttgtgcgtg cccttgaagc cgacctcggt
     3061 catcaccttc tgcatctcgg cgcggatgcg cgcgacttcg gagagtccct tctcgtgaac
     3121 ctgctccggc gtcaggtcgg tggtcgtgta gcggcgcgcg aggaaggcgt aggccgcttc
     3181 gccgtcgggc cactgccaga cgccgacttg agggaaggag gcgggcaggt actcgtcgtc
     3241 gaagtaggtc ttgagctgac ggtaggaagg gatgacgtgc tgcgagacgg cctcgcgcgc
     3301 ttcgcgcgtg aggcgttcgc gctcttgcgc gggcacatcg tcgtgaaact tcttgaaggg
     3361 cgcgtagaag gggctgtctt cgggtcgcgc gacgagctgc ttgtcgatct gcgcgggcac
     3421 gcgcttgagg acttcgtgcg gccagagctt tttggcgcgc acgccctcgc gcatgagcgc
     3481 gagcgtctgc tccatgtagc gcgggagctg ccgcagacgc gccacccagt cgtcgtagtc
     3541 cttgacggtc tggaagcgca gcagctcggt cagctcgtcg gcggtctgga tgccgccgcg
     3601 ctggttgacg ggcagcaggt agaggcggaa gccgtgctct tcgatgtcgg tttcgaggtc
     3661 tttgcggaag aggtcgtggt tgagctgatc cgccgccgag agccgcgcgc ggtcgatggc
     3721 cttgaggcgc gcgagcgctt cctgccggtg gcggtggcgg gcctcgatcg cttccagccc
     3781 gacgtcctcc cagcggtcgt tgtagcgccg gtcgccgagc gacgaggccc acgtcgggtt
     3841 ctcgcgcatc gtccactccc actcgtcctc gaacagcttg tgcagagctt tcgcggcggg
     3901 cgcgttcctg tccgtcgtct tcatcgcgcc tcctccgcgc gccgcggact gtcgcgccgc
     3961 gaccggcagc acaggcgaaa ggagtacggc ggcggcgagc aacgcgggca gccggccctt
     4021 gaatcgagag cgcatcgcaa gtgaacctcc gttcggcgcg gtttgcggga tggaagaaac
     4081 ctagcgagag acggggggaa gaatacaaca gaaaagcagg aagccagaag tcaggagtcg
     4141 gaagtcggaa taagaaacgt ggccgccgcc gttcttccga ctcctgactt ctgacttccg
     4201 atttccgact tccttttcgt ctgctttcgc atccaagccg agccatgagt gtttcgatga
     4261 acgagctgaa gacgtatgcg agttacgcgc ggcggttcgg gcggcgcttc atgaaggagc
     4321 gcgtgcgcga gctgcgcgtg ccggtggcgg cgccgccagt aactgaccca tggtttagcg
     4381 cgctcatcga cctgacccta gggctgacga gcgagggcgc taaaccatgg gtcagttact
     4441 ggcggcg
//
LOCUS       DBNGOH010001088         3106 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4930008, whole genome shotgun sequence.
ACCESSION   DBNGOH010001088 DBNGOH010000000
VERSION     DBNGOH010001088.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3106)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3106)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3106
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4930008"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..587)
                     /locus_tag="AC159P_21240"
     CDS             complement(<1..587)
                     /locus_tag="AC159P_21240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011515792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HIR3698028.1"
                     /translation="MAVNEERLNQLLGRFVADFGAAFHAGLVVIGESLGLYKALAEGP
                     LTSAELAERTGTHERYVREWLNSQAAGGYVEYDADAGRYSLTEEQAFALADERSPAYL
                     PGAFLLAVSALRAVPQITERFRTGEGFGWHEHDHGLFRGTELFFRPGYAANLIGSWLP
                     ALDGVEEKLKAGAKVADVGCGLGASTLLMAEAYPAS"
     gene            complement(840..1271)
                     /locus_tag="AC159P_21245"
     CDS             complement(840..1271)
                     /locus_tag="AC159P_21245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006451695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3698029.1"
                     /translation="MIAKREPTLVLAGAGSLAASILHLACVAGGADWYRALGAGERMA
                     RMAARGHWYPTVVTLCIAATLGAWALYAWSGAGLIRRLPLLRLGLCVITGVYLLRALA
                     FVPLQAFFPGNSAAFWFWTSGVCLGLGLLHAHGLRRAWSRL"
     gene            complement(1435..1947)
                     /locus_tag="AC159P_21250"
     CDS             complement(1435..1947)
                     /locus_tag="AC159P_21250"
                     /inference="COORDINATES: protein motif:HMM:NF024952.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HIR3698030.1"
                     /translation="MAVKLMPVSRRQLSMYVPQDAARDIEAVRRVVDPIQSRLIPAHV
                     TLCREDELGDLASVRARLRRAPFNPLTLRFGKPVAFAGHGLLLECVEGEEHFRAVREY
                     LLASSRVREQRPHITLAHPRNPKAGGNSLSNTSRLPEVIEVTFPSIYLIEQEKGGPWQ
                     LLEMYELRDV"
     gene            complement(2122..2838)
                     /locus_tag="AC159P_21255"
     CDS             complement(2122..2838)
                     /locus_tag="AC159P_21255"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005814256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3698031.1"
                     /translation="MMSDKPVAFDAYETLAEAYAAVIDTKPHNAYCERPATLSLLPDV
                     GNKRVLDAGCGPGIYSEWLIEHGAEVVAVDASLKMVELAKRRLGGAAEVRQADLGQPL
                     DFLGDSSFDVVLSPLVLEYVEDWRGAFAECYRVLRPGGHLIFSVTHPFSDYLYYKSVN
                     YHETELVGCEWQGFAPVKVHMPSFRRPLSAMLNPLLEAGFYLERIVEPKPTEEFKEAD
                     PKHYEELSRLPCFLCVRARK"
     gene            complement(2930..3094)
                     /locus_tag="AC159P_21260"
     CDS             complement(2930..3094)
                     /locus_tag="AC159P_21260"
                     /inference="COORDINATES: protein motif:HMM:NF022317.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2784 family protein"
                     /protein_id="HIR3698032.1"
                     /translation="MTYRALADLVLLTHIAFAIFTVLGGLLVLRRRSLLWLHLVSRLC
                     ARRAGAASGG"
BASE COUNT          573 a         1028 c          993 g          512 t
ORIGIN      
        1 gaggccgggt aggcctcggc catcaggagc gtcgaggccc cgaggccgca gccgacatcc
       61 gccaccttgg cccccgcctt cagcttctcc tcgacgccgt cgagcgcagg gagccaggag
      121 ccgatcaggt tggcggcgta gcccggtcgg aagaacagct cggtgccgcg gaacagcccg
      181 tggtcgtgct cgtgccagcc gaagccctcg cccgtgcgga atctctccgt gatctgcggc
      241 acggccctca gggccgagac ggcgagcagg aaggcgcccg gcaggtaggc cgggcttcgc
      301 tcgtcggcca gagcgaaggc ttgctcctcc gtgagcgagt agcgccccgc gtcggcgtcg
      361 tactcgacgt agccgccggc ggcctgcgag ttgagccact cgcggacgta gcgctcgtgc
      421 gtgccggtgc gctcggccag ctcggcggag gtcagcggcc cctcggccag cgccttgtag
      481 aggcccaggc tctcgccgat gacgaccagt ccggcgtgga aggcggcgcc gaagtcggcg
      541 acgaaccgcc ccagcagttg gttgagtctc tcctcgttaa cggccataaa ccctccctta
      601 cagaatagtg acggaaatgg gcaccgcgcg gcccgcggtc tacgccgggg cgcgggcgac
      661 aagataaatg agcggcgggc gggattcaaa caccgcgccc cgctgcggcg cgtccgtgtg
      721 tggccggctt cggccaccgc gtccggcccc ccgtgaggat agtgtgttag agcgggggga
      781 atatacgtgg gggaggggag ggtgtcaacg gcgaggggga gggtactgcc ggaccaggtt
      841 cacagacggg accatgcccg acgcagcccg tgcgcgtgca gcagcccgag gcccaagcag
      901 acgcccgaag tccagaacca gaaggcggca ctgttgccgg ggaagaaggc ctggagcggc
      961 acgaaggcga gcgcgcgcag gaggtagacc cccgtgatga cgcacaggcc caggcgcagc
     1021 aacggcagcc gccggatcag cccggcgcca gaccaggcgt acagcgccca ggcgcccagg
     1081 gtcgccgcga tgcagagagt cacgaccgtg ggataccaat ggccgcgggc ggccatccgc
     1141 gccatgcgct ccccggcccc gagggcccga taccagtccg caccgcccgc gacgcacgcc
     1201 agatgcagga tcgatgccgc gagcgacccg gcgcccgcga gaacaagcgt tggttctcgc
     1261 ttcgcgatca tgtgcctgtg tgtcctaacg cccggtgtcg gcggcgcgct cgatactttg
     1321 atgacaatag gcaacgtgat ttgcgcgtcc gctgcctgcc gtcgttgggc tgcgccgtgt
     1381 gtggccggca cccgcgcacc gggctgatgt ccgccgccca tccgttggtt ccggttagac
     1441 gtcccgcagc tcgtacattt ccaacaactg ccacggcccg cccttctcct gctcgatgag
     1501 atagatgctc gggaatgtca cctcgataac ttcgggcagc ctcgacgtgt tgctcaacga
     1561 attacctccc gccttcgggt tcctcgggtg cgccagcgtg atgtggggcc tctgctccct
     1621 gacgcgactc gaagcgagca gatactcgcg caccgcgcgg aagtgctcct cgccttcgac
     1681 gcattccagc agtaggccgt gcccggcaaa ggccaccggc ttgccgaacc gcagggttag
     1741 cgggttgaat ggggcgcggc ggagtcgcgc ccggaccgac gcaaggtcgc cgagctcatc
     1801 ctccctgcac aacgtcacgt gcgccgggat caggcggctt tggatcgggt caacgaccct
     1861 tcgcaccgcc tcgatgtctc tcgcggcatc ctgcggaaca tacatgctca gttgcctgcg
     1921 gctcaccggc atcagcttga cggccatcca ttcccgacgc ggcgcgtggt tcgaccatgc
     1981 ggcttcagga gtttcccggc ggggcgccgg agtgctcggc ctgtgaggac acaggctgtt
     2041 tatcccctgc cgcctaccgc cgcggctaac aagagctact tttggggcgg tcgggtgact
     2101 gccgctgtgc ggcagcggcc ttcacttccg agcacggacg catagaaaac acggcagccg
     2161 tgacagctcc tcataatgct tcgggtctgc ctctttgaac tcttccgtcg gcttcggctc
     2221 gactatcctt tccaggtaga agcccgcttc gagaagcgga ttcagcatcg cactcagggg
     2281 gcggcggaaa ctcggcatgt gaactttgac cggcgcaaaa ccctgccact cacaaccgac
     2341 cagctccgtc tcgtgataat taaccgactt gtagtacagg taatcggaga aaggatgagt
     2401 gacggaaaat ataaggtggc ctcccggacg caggactcta tagcactccg cgaaagcgcc
     2461 gcgccaatcc tcgacgtatt ccaagacgag cggactcagc acaacgtcga aggatgagtc
     2521 gcccagaaag tcgaggggtt ggcccaagtc cgcctgccgc acctcagccg ccccgccgag
     2581 cctccgcttt gctaactcga ccattttcag gctggcatcg acggcaacga cttcagcgcc
     2641 atgttcgatc agccactcgg aataaatccc cgggccacac cctgcgtcga ggactctctt
     2701 gtttccgaca tccggcaaga gtgagagggt ggccgggcgt tcgcaatacg cgttatgcgg
     2761 tttcgtgtcg atgacagccg cgtaagcctc ggccagagtc tcgtacgcat cgaaagcgac
     2821 aggtttatca ctcatcatcg ttcgaggtgt cagccacttc tttaccacgg cgcagcctga
     2881 cttcgaatca ggtggagatt tgtccgccta acttattcgg gcggacattt caaccgcccg
     2941 aggccgcccc cgcccgccgt gcacacaacc tcgaaacaag gtgtagccac aagagagagc
     3001 gccggcgtaa gacgagcaaa cctcccagca ctgtaaagat ggcgaaagca atatgtgtca
     3061 gtaagacgag atcagctaag gcccggtagg tcattgcgtc ctacag
//
LOCUS       DBNGOH010001089         1931 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_532258, whole genome shotgun sequence.
ACCESSION   DBNGOH010001089 DBNGOH010000000
VERSION     DBNGOH010001089.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1931)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1931)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1931
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_532258"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1817
                     /locus_tag="AC159P_21265"
     CDS             <1..1817
                     /locus_tag="AC159P_21265"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HIR3698033.1"
                     /translation="YVAQGQPQGAQIFVKWLDSPGETQLTRVERAPSNLAWSPDGKHI
                     AFNMLVPGNPGLKIKMPERPAGAKWVEPPRVVDRMNYRADGSGWRPEGFTHIFVVSEQ
                     GGSPRQLTDGDYQHGAPEWMRDSRALVFSGVRKDGAEYIRGAADIYTVPLAGGAIRAL
                     TDRSGPDTNPTVSPDGKSIAYTGFDQTDNTYTVTKLYVMNADGTNKRVLTQSFDRSPA
                     GLFWTEGGLYFVAEDRGAQNLWHHPVEKGGPAQVTQGPHILSVTSVSRTGLAVGTRSA
                     ANAPPDVVAFELKSPAGLRRLTAVNEDLLEGRKLGEVEEIWYDSVGGVLQQNCTAIDS
                     APLAPRPAGCDKARVQGWVVKPPDFDPSKKYPLMLYIHGGPHSMYGVGFNFEFQNHAA
                     EGYVVLYTNPRGSTGYGAAFGNAINNAYPGQDYDDLMRGVDEVLRRGYIDERQLFVTG
                     GSGGGVLTTWIVGHTDRFAAAVAMKPVTNWYSFVGTTDGADWYYNFKRLPWEDPAEHL
                     RRSPLSYVGNVRTPTMILTGDLDLRTPLEQTEQYYRALKMRKVDTAMVRLTDEFHGFN
                     AEFGLRHPSNRVSQILFLRGWFEKYRNKGQATTTTTDR"
BASE COUNT          354 a          681 c          618 g          278 t
ORIGIN      
        1 cgtacgtggc gcagggccag ccgcagggcg cgcagatttt cgtgaagtgg ttggactcgc
       61 cgggcgagac gcagttgacg cgggtggagc gcgcgccgtc gaacctcgcg tggtcgccgg
      121 acggcaagca tatcgctttc aacatgctgg tgccgggaaa ccccggcctc aagatcaaga
      181 tgccggagcg gcccgcgggc gcgaagtggg tcgagccgcc gcgggtggtt gaccggatga
      241 actaccgcgc ggacggctcg gggtggcggc ccgaaggctt cacgcacatc ttcgtcgtct
      301 cggagcaggg cggcagcccg cgtcaactga cggacggcga ctaccagcac ggcgcgccgg
      361 agtggatgcg cgactcgcgc gcgctggtct tcagcggcgt gcgcaaggac ggagccgagt
      421 acatccgcgg cgcggcggat atctacacgg tgccgctggc gggcggcgcc atccgcgcgc
      481 tcacggatcg cagcggcccg gacaccaacc ccacggtctc gcccgacgga aagtcgatcg
      541 cctacacagg cttcgaccag acggacaaca cgtacaccgt caccaagctc tacgtgatga
      601 acgccgacgg cactaacaag cgcgtgctga cgcagagctt cgaccgctcg cccgcgggcc
      661 tcttctggac ggagggaggc ctctacttcg tcgccgaaga ccgcggcgcg cagaacctct
      721 ggcatcaccc ggtcgagaag ggcggccccg cgcaggtcac gcaggggccg cacatcctga
      781 gcgtcacctc ggtcagccgg acggggcttg cggtcggcac gcgctccgcc gcgaacgccc
      841 cgccggacgt ggtcgccttc gagctgaaaa gccccgccgg gctgcgccgc ctcaccgccg
      901 tcaacgaaga ccttttggaa gggcgcaagc tcggcgaggt cgaggaaatc tggtacgact
      961 cggtgggcgg cgtccttcag cagaactgca cggccatcga ctcggcgccg ctcgcgccgc
     1021 ggcccgccgg ctgcgacaag gcgcgggtgc agggctgggt cgtcaagccg cccgacttcg
     1081 acccctcgaa gaagtacccg ctgatgctct acatccacgg cggcccgcac tcgatgtacg
     1141 gcgtcggctt caacttcgag ttccagaacc acgcggcgga aggctacgtc gtgctctaca
     1201 ccaacccgcg cggctcgacc ggctacggcg cggccttcgg caacgccatc aacaacgcct
     1261 atccgggtca ggactacgac gacctcatgc gcggcgtcga tgaagtcctg aggcgcggct
     1321 acatcgacga gcgccagctc ttcgtcacgg gcggctcggg cggcggcgtg ctgacgacgt
     1381 ggatcgtcgg ccacaccgac cgcttcgccg ccgccgtcgc catgaagccc gtcaccaact
     1441 ggtactcctt cgtcggcacc acggacggcg cagactggta ctacaacttc aagcggctgc
     1501 cgtgggaaga cccggcggag cacctgcgcc gctcgcccct ctcttatgtc ggcaacgtca
     1561 ggacgccgac catgatcctc accggcgacc tcgatttgag aaccccgctc gaacagaccg
     1621 agcagtacta ccgcgccctc aagatgcgca aggtggacac cgcgatggtg cgcctcaccg
     1681 acgagttcca cggcttcaac gccgagttcg gtctgcgcca cccctccaac cgcgtctccc
     1741 aaatcctctt cctccgcggc tggttcgaga agtaccgcaa caaggggcag gcgacgacaa
     1801 cgacgacgga caggtgaatc ttcgtggcgc gaaaagctgc ctcaccaaaa acaagaagac
     1861 cttcaaaggc tgcgaagcgc agtgacgccg ccgagacttc tctttacact gtcttcatca
     1921 gccactcctc c
//
LOCUS       DBNGOH010001090         2988 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1083496, whole genome shotgun sequence.
ACCESSION   DBNGOH010001090 DBNGOH010000000
VERSION     DBNGOH010001090.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2988)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2988)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2988
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1083496"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1480)
                     /gene="recJ"
                     /locus_tag="AC159P_21270"
     CDS             complement(<1..1480)
                     /gene="recJ"
                     /locus_tag="AC159P_21270"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HIR3698034.1"
                     /translation="MTMRAASRNAHDDRRWVVRADDPERARALARALGVSTVVAGLLI
                     ARGVETEREARRVLHPSFAQLHDPYLMLGMREAVARVVRAIDAGERILIYGDYDVDGT
                     TGTVVLRRALQVLGARTGYHVPHRFTEGYGIRQDVLERAQAEGYTLVISVDCGIRAHE
                     PLEWAEGHGLDVIVTDHHLPDDDEGAPPAFAVLNPNQRGCQYPDKNLAGVGVAFKLAH
                     ALFRERGRESLVSGFLKVVAIGTVADVAQLVGENRAIVALGLADLWKATNPGLRALME
                     VSGSGDGRGMRASDLGFRLGPRINAAGRMDAARAVVELFETDSKEEARRLAEHLDARN
                     RERRVVQQEITERAIAELEFGGLCDDRVAVVAGTGWHRGVIGLAASKIVERLRRPAVV
                     ISLDADGTGHGSARSTDDFHMLDALTACADLLEGFGGHAHAAGLAVRRENVAELRRRL
                     NEHAAAHLREEDFAPSLHVDMELPAEALSLELCDELARLEPFG"
     gene            complement(1477..1803)
                     /locus_tag="AC159P_21275"
     CDS             complement(1477..1803)
                     /locus_tag="AC159P_21275"
                     /inference="COORDINATES: protein motif:HMM:NF018894.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HIR3698035.1"
                     /translation="MSDEPKGKETPPAQRPRSPRVPVNFTVELEGRSAADEPFQVQAE
                     AVRVSRGGATLVTDAAVSTGALVRLTPPFGRALEAEVNGVWKDEADGRQRIGVRLLDP
                     NGWFAE"
     gene            complement(2017..2934)
                     /locus_tag="AC159P_21280"
     CDS             complement(2017..2934)
                     /locus_tag="AC159P_21280"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012466649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HIR3698036.1"
                     /translation="MTILQAIILGIVQGLTEFIPISSTAHLVFASRLTGLYAGVEQTL
                     RAQQTTATIAVIQLGTLLAVFVYFARDIVNITTAFVRDHLALLRGGGGAEGQQGGGGF
                     WSRLSSEARLGWLIFIGSLPVGVFGLLFKDFIEGTFTKNLWVIATMMVVVALLLLVAE
                     KVGARRKDVEDLGLVDALAVGFAQVLALVPGASRSGSTIMGGLFAGEKRETAARFSFL
                     LSIPAIAASGLLELREALEKLPPDSALPLAVATVVSGVVGYASIWFLLRFLRTHSTGV
                     FIGYRLVVGGIILALLAAGILNPLEGMAG"
BASE COUNT          445 a          999 c         1104 g          440 t
ORIGIN      
        1 cgccgaaggg ttcgagccgc gccagctcgt cgcagagttc gagcgagagc gcctcggcgg
       61 gcagctccat gtcaacgtgc aggctgggcg cgaagtcctc ttcgcggagg tgcgcggcgg
      121 cgtgctcgtt gaggcggcgg cgcagctcgg ccacgttctc gcggcggacg gcgaggcccg
      181 cggcgtgcgc gtggccgccg aagccttcga gcaggtcggc gcaagcggtg agcgcgtcga
      241 gcatgtggaa gtcgtccgtg ctgcgcgccg agccgtggcc cgtgccgtcg gcgtcgagcg
      301 agatgacgac cgcggggcgg cgcaagcgtt cgactatctt cgaggccgcc agcccgatga
      361 cgccgcggtg ccagcccgtc ccggcgacca cggcgacgcg gtcgtcgcag aggccgccga
      421 actcaagctc ggcgatggcg cgctcggtga tctcctgctg cacgacgcgg cgctcgcggt
      481 tgcgcgcgtc gaggtgttcg gcgagacgcc gcgcctcctc tttgctgtcc gtctcgaaca
      541 gctcgacgac ggcgcgcgcc gcgtccatgc gtccggcggc gttgatgcgc gggccgaggc
      601 ggaagccgag gtcggaggcg cgcatcccgc gaccgtcgcc gctgcccgag acctccatca
      661 gggcgcgcag gccggggttc gtggccttcc acaaatcggc gaggccgagc gcgacaatcg
      721 cgcggttctc gccgacgagc tgcgccacgt ccgcgacggt gccgatggcg acgaccttga
      781 gaaagccgct gaccaggctc tcgcgcccgc gttcgcggaa gagcgcgtgc gcgagcttga
      841 aggcgacgcc gacgcccgcg agattcttgt ccgggtactg gcagccgcgc tggttggggt
      901 tcaagacggc gaaggcgggc ggcgctcctt cgtcgtcgtc gggcaggtgg tggtcggtga
      961 cgatcacgtc gaggccgtga ccctcggccc actccagcgg ctcgtgcgcg cggatgccgc
     1021 agtccacgct gatgacgagc gtgtagcctt cggcctgcgc gcgttcgagc acgtcctgcc
     1081 ggatgccgta gccttcggtg aagcggtgcg ggacgtggta gccggtgcgc gcgccgagga
     1141 cttgcagcgc gcggcgcagg acgacggtgc cggtcgtgcc gtccacatcg tagtcgccgt
     1201 agatgagaat gcgctcgccc gcgtcgatgg cgcggacgac gcgcgcgacc gcctcgcgca
     1261 tgccgagcat gaggtacggg tcgtggagtt gtgcgaagga tggatggagg acgcggcgcg
     1321 cttcccgttc ggtttcgacg ccgcgggcga tgagcaggcc ggcgaccacg gtcgagacgc
     1381 cgagggcgcg ggccagcgcg cgggcgcgtt cgggatcgtc ggcgcgcacg acccagcgcc
     1441 ggtcgtcgtg cgcgtttcgg ctcgcggccc tcatcgtcat tcggcgaacc agccgttggg
     1501 gtcgagcagc cggacgccga tgcgctgccg cccgtccgcc tcgtccttcc acacgccgtt
     1561 gacctcggct tcgagcgcgc gcccgaacgg cggcgtcagg cgcacgagcg cgcccgtcga
     1621 gaccgcggcg tcggtgacga gcgtcgcgcc gccgcgcgag acgcgcaccg cctcggcctg
     1681 cacctggaac ggctcgtcgg cggcgctgcg gccttcaagc tcgaccgtga agttgacggg
     1741 cacgcgcggg ctgcgcggcc tctgcgcggg cggcgtctct ttgcctttcg gttcgtcgct
     1801 catggagatg tttcgccctc caacacacac gggggatttc gtcgttcggt cgttgagcgg
     1861 gggtacggac aagtgtaacc gaatagcgac gattccgcct atcggcaaga aggggcgaca
     1921 gggagacggg gtgacgggga gacggggaga aggacagtta agctcatctc cccgtctccc
     1981 cttcacccct tcaccccgtc gccccgtctc gccttcttaa ccggccatgc cttcgagcgg
     2041 gttgaggatg cccgccgcga ggagcgcgag gatgatgccg ccgacgacga ggcggtagcc
     2101 gatgaagacg ccggtcgagt gggtgcgcag gaagcggagg aggaaccaga tcgaggcgta
     2161 gccgacgacg ccggagacga ccgtggcgac ggccagcggg agcgcgctgt cgggcgggag
     2221 cttttcgagc gcctctctga gttcgagcag gccgctcgcg gcgatggccg ggatcgagag
     2281 caggaacgag aagcgcgccg ccgtctcgcg cttctcgccc gcgaacagac cgcccatgat
     2341 ggtcgagccg gaacggctcg cgcccggcac gagcgccagc acctgcgcga agccgaccgc
     2401 cagcgcgtcc actagcccca ggtcttccac gtccttgcgc cgcgcgccga ccttctcggc
     2461 gacgagcagc agcagcgcca ccaccaccat catcgtcgcg atgacccaca ggttcttcgt
     2521 gaacgtgccc tcgatgaagt ccttgaagag cagcccgaag acgccgaccg gcagcgagcc
     2581 gatgaagatc agccagccca gccgagcctc gctcgacagg cgcgaccaga aaccgccgcc
     2641 gccctgttga ccctccgcgc cgccgccgcc gcggagcagc gcgaggtggt cgcggacgaa
     2701 ggccgtcgtg atgttcacga tgtcgcgcgc gaagtagacg aagacggcga gcagcgtgcc
     2761 gagctggatg acggcgatgg tggccgtcgt ctgctgcgcg cgcagggtct gttcgacccc
     2821 cgcgtagagg cccgtcaagc ggctggcgaa gacgaggtgc gccgtcgagc tgatggggat
     2881 gaactcggtc agcccctgta cgatgccgag gatgatggct tgaagaatgg tcatgaaggg
     2941 tttttaggtt tccgaatttc cgcttgcgct ggctgtcaag gcgaggca
//
LOCUS       DBNGOH010001091         1722 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_532566, whole genome shotgun sequence.
ACCESSION   DBNGOH010001091 DBNGOH010000000
VERSION     DBNGOH010001091.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1722)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1722)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1722
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_532566"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="AC159P_21285"
     CDS             complement(<1..233)
                     /locus_tag="AC159P_21285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3698037.1"
                     /translation="MPQYTPLELSRRNFLGRAGAVVAGLSALNVSALALAGCRRQEQP
                     LTAVPSVMPPATSDTPKNLSWQTVITSKDEPGR"
     gene            complement(357..1568)
                     /locus_tag="AC159P_21290"
     CDS             complement(357..1568)
                     /locus_tag="AC159P_21290"
                     /inference="COORDINATES: protein motif:HMM:NF016648.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HIR3698038.1"
                     /translation="MFSKATFLKMFGNHVKQHLSAYAHGELAAGEAERVAAHLAACAR
                     CRAEFEEIKFGIRLAESLPAAAAPAALWDGIEAALSSTSKAQRHDASPTKQRDTLSLP
                     PPPRFGFLHGWRRFAIVAAAACFVLVAGAAWFYFAPPRAGWEVARLSGVPLIDSERVD
                     STGRLAVGEVLETDDKSSAQIKVANIGHVEVDPNTRLRLIETRVTEHRLELQRGRLHA
                     RIWAPPRLFFVNTPSAVAADLGCAYTLEVDDRGGSRLHVTSGWVALEAGARDSIVPAG
                     AACVTRPGTGPGTPYFEDAPAPLVAALSRFDFEQGGAAALDVVLAEARPRDTLTLWHL
                     LSRVDAGEQREKVYERLAALVPPPDGVTRDGVLRLDRAMLDRWKDDLESKWNFENLPT
                     LKKALRKIMTK"
     gene            complement(1568..1717)
                     /locus_tag="AC159P_21295"
     CDS             complement(1568..1717)
                     /locus_tag="AC159P_21295"
                     /inference="COORDINATES: protein motif:HMM:NF019886.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HIR3698039.1"
                     /translation="MKYFEELSYEEIAEVLGCSKGTVASRLNRGHKILARKLKHLRGS
                     PVHTG"
BASE COUNT          268 a          552 c          620 g          282 t
ORIGIN      
        1 tggcggcccg gctcgtcctt cgacgtaatg acggtctgcc acgagaggtt cttcggcgta
       61 tcacttgtcg cgggcggcat gacggacggg acggcggtga gcggctgctc ctggcggcgg
      121 caacccgcga gggccagcgc gctcacgttg agcgcggaga ggccggcgac cacggcgccc
      181 gcgcgcccga ggaagttgcg gcgagaaagc tcaagcggtg tgtactgcgg catggagacc
      241 tcctagcgat tgcggattgt ggattgcgga ttggcgtgct gcttcggctt gacgcctgat
      301 tttggaagtt caactttcat gttcgaacaa tccgcaatcc gcattccgca atcgtttcac
      361 ttcgtcatga tcttgcgcaa cgctttcttg agcgtcggca ggttctcgaa gttccacttg
      421 gattccaaat cgtctttcca gcgatcgagc atcgcgcggt cgaggcgcag gacgccgtcg
      481 cgggtcacgc cgtcgggcgg gggcacgagc gcggccagtc gttcgtagac cttttcgcgc
      541 tgctcgcccg catcgacgcg cgagagcagg tgccagagtg tgagcgtgtc gcgcgggcgc
      601 gcctcggcca gcaccacgtc gagcgccgcc gcgccgccct gctcgaagtc gaacctggag
      661 agcgcggcga cgagcggggc gggcgcgtct tcgaagtagg gcgtgccggg gcctgtgccg
      721 ggccgcgtga cgcaggccgc gcccgccggg acgatggagt cgcgcgcgcc cgcttcgagc
      781 gcgacccagc ccgaggtgac gtgcagacgg ctgccgccgc ggtcgtccac ttcgagcgtg
      841 taggcgcagc cgaggtcggc ggccaccgcc gacggcgtgt tgacgaagaa gaggcgcggc
      901 ggcgcccaaa tcctcgcgtg caggcgcccg cgctgaagct ccagacggtg ctcggtcacg
      961 cgcgtctcga tgaggcgcag ccgcgtgttc ggatcgacct cgacgtggcc gatgttggcg
     1021 accttgatct gcgcgctcga tttgtcgtcc gtctccagca cctcgccgac ggcgagcctt
     1081 ccggtcgagt ccacgcgctc ggagtcgatg agcggcacgc ccgaaagtct tgccacctcc
     1141 cagcccgcgc gcgggggcgc gaagtagaac cacgcggcgc ccgcgacgag gacgaaacag
     1201 gcggcggcgg cgacgatggc gaaacgtcgc cacccgtgta agaagccgaa gcgcgggggc
     1261 ggggggaggg aaagggtgtc gcgttgtttc gtcggcgaag cgtcgtgccg ctgcgccttc
     1321 gaagtcgatg agagcgccgc ttcgatgccg tcccagagcg cggcgggggc ggcggcggcg
     1381 ggcaggctct cggcgaggcg gatgccgaac ttgatctcct cgaactcggc gcggcaccgt
     1441 gcgcaggcgg cgaggtgcgc cgcgacgcgc tcggcctcgc ccgccgcgag ttcgccgtgt
     1501 gcgtaagccg aaaggtgttg tttgacgtgg ttgccgaaca tcttcaaaaa cgtcgccttc
     1561 gaaaacatca acccgtgtga accggagagc cgcgcaggtg tttgagcttg cgcgccagaa
     1621 tcttgtgccc gcgattgagc cgcgaggcca ccgtgccctt cgagcagccg agcacctcgg
     1681 cgatctcctc gtaggacagc tcctcgaaat atttcaggag ca
//
LOCUS       DBNGOH010001092         2843 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1631698, whole genome shotgun sequence.
ACCESSION   DBNGOH010001092 DBNGOH010000000
VERSION     DBNGOH010001092.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2843)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2843)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2843
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1631698"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            96..167
                     /locus_tag="AC159P_21300"
                     /pseudo
     CDS             96..167
                     /locus_tag="AC159P_21300"
                     /inference="COORDINATES: protein motif:HMM:NF024645.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
     gene            174..2339
                     /locus_tag="AC159P_21305"
     CDS             174..2339
                     /locus_tag="AC159P_21305"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR3698040.1"
                     /translation="MSGRDSAAESVPTAERVGGKANPTISDKEALVGRVLDGKYEILS
                     PLGEGGMGAVYRARRVHIGDEVAVKVLHPKYVNDETLLERFRREARAAAQLHHPYIVT
                     IHDYGEAGGTAGFAYIVMELVQGEPLRGLLLAEGKLGAPRAVSLMRDICAGVGAAHRR
                     GIVHRDLKPDNIIVLPMDDDHERERVKVVDFGIAKLRDMASDSTLTQAGAVVGTPYYM
                     SPEQCRGESLDARADVYSLGALLYEMLAGTPPFTAPSITGVIAKHLTEAPPRLPDSLN
                     VPEALQHAIMRSLSKDPEGRQKDASEFAREIQSAAASKSAEESKPTVVAPPPGDAPPP
                     FSIGQQPAPVVPLQPPQPTEAPPPPVPTAHPHTPSQTDPRQFAPQTYGQQHTHAPPHT
                     QQPTHGQHPPHQRHAQPLPATPPRRKSRAPLVVGLLVLSVIVVAVAGIAGLIYLGSGE
                     EDGTASISNADRANTRPTATPATETNDNADDTNTNTNTGPPISDAMSRAESKVVSDTL
                     LKREDLAALSPAELRILRNGIFARHGRVFQDEDLKGYFEGRSWYSPNNAFSDSMLTSA
                     DRANAELIKSVEAGEPAAPPADAAAIKTDIGVTLEAWASTTRAKLLDLHMIYYADTLD
                     TYYRRTDVPASLVRADRARAFETYDTMDVQLSGVKINLDSSGTRATVTLDKTWEFASE
                     DKDSNGSVRQQLTLSKIDGRWLITGEKDLEVHYSNSVKN"
     gene            2391..>2843
                     /locus_tag="AC159P_21310"
     CDS             2391..>2843
                     /locus_tag="AC159P_21310"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008099557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3698041.1"
                     /translation="MSETTFNLRRAAAGDREVLVEFNLAMARETEAKELSPEVLRAGV
                     AHFIERPQYGFYVVAEDGGQVVGSLMITYEWSDWRDGLFWWIQSVYVRPSARRRGVYR
                     RLYEHVKRLAAADDSEVRGFRLYVEKENRVAQATYEQLGMSETHYLMYE"
BASE COUNT          518 a         1060 c          891 g          374 t
ORIGIN      
        1 ggatgcccaa gcacgcactg accagcgact cgacgccctg atcgatatta ttcggaatga
       61 gcgcggcggg cagttgccgg acaactgatc cttatatgaa taccaattgt ccacagtgtg
      121 ggcagccttt gcgggagggc gcgctgttct gtacgaagtg cggggcgcgc actgtgagcg
      181 ggcgcgactc ggcggcggag tcggtgccga ccgccgagcg cgtcggcggc aaggcgaacc
      241 cgaccatctc cgacaaggag gcgctcgtcg ggcgcgtgct cgacggcaaa tacgaaatcc
      301 tgtcgccgct cggcgagggc gggatgggcg cggtctaccg cgcgcggcgc gtccacatcg
      361 gcgacgaggt ggccgtcaaa gtcctccacc cgaaatacgt caacgacgag acgctgctcg
      421 aacgcttccg gcgagaggcg cgcgccgccg cgcagctcca ccacccgtac atcgtcacca
      481 tccacgacta cggcgaggcg ggcggcaccg ccggcttcgc ctacatcgtg atggagctgg
      541 tgcagggcga gcctttgcgc gggctgctgc tcgcggaggg caagctcggc gcgccgcgcg
      601 ccgtctcttt gatgcgcgac atctgcgcgg gcgtcggcgc cgcgcaccgg cgcggcatcg
      661 tccaccgcga cctcaagccc gacaacatca tcgtgctccc catggacgac gaccacgagc
      721 gcgagcgcgt caaggtcgtg gacttcggca tcgccaagct gcgcgacatg gcctcggact
      781 cgacgctgac gcaggccggc gccgtcgtcg gcacgcccta ctacatgtcg cccgagcagt
      841 gccgcggcga atcgctcgac gcgcgcgcgg acgtgtactc cctgggcgcg ctgctctacg
      901 agatgctcgc gggcacgccc ccgttcacgg ccccttccat cacgggcgtc atcgccaagc
      961 acctgaccga agcgccgccg cgcctgcccg acagcctgaa cgtccccgaa gcgctacagc
     1021 acgcgatcat gcgctcgctc tcgaaagacc cggaggggcg gcagaaggac gcctcggagt
     1081 tcgcgcgcga gattcaaagc gccgccgcga gcaagagcgc ggaagagagt aagccgaccg
     1141 tcgtcgctcc gccgccgggc gacgcgccgc cgcccttctc gatcggtcaa cagcccgcgc
     1201 ccgtcgtgcc cttgcagccg ccgcagccga cagaggcgcc gcccccgcca gtcccgaccg
     1261 cgcacccgca cacgccctcg cagacagacc cgcgccagtt cgcgccgcag acctacggcc
     1321 agcagcacac gcacgcgccg ccgcacacgc agcagccgac gcacggccag cacccccctc
     1381 atcaacgaca cgcgcaaccg ctgcccgcaa cgcccccgcg taggaagtcg cgcgcgccgc
     1441 tcgtcgtcgg cctcctcgtc ctctccgtca tcgtcgtcgc ggtcgcaggc atcgcggggt
     1501 tgatctacct cggcagcggc gaagaagacg gcacggcctc catctcgaac gccgaccgcg
     1561 cgaacacgcg ccccaccgcg acccccgcga ccgagaccaa cgacaacgcg gacgacacga
     1621 acaccaacac gaacaccggc ccgccaatct ccgacgcgat gagccgcgcc gagtcgaagg
     1681 tcgtctccga cacgctcctc aagcgcgaag acctcgccgc gctcagccct gccgagcttc
     1741 gcatcctgcg caacggcatc ttcgcgcgcc acggtcgcgt ctttcaggac gaagacctga
     1801 aaggctactt cgaaggccgg agctggtact cgccgaacaa cgccttcagc gactccatgc
     1861 tcaccagcgc cgaccgcgcc aacgccgagc tgatcaagtc cgtcgaggcc ggcgagcctg
     1921 cggcgccgcc cgccgatgcc gccgccatca agaccgacat cggcgtcacg ctcgaagcgt
     1981 gggccagcac gacgcgcgcc aagctgctcg acctgcacat gatctactac gccgacacgc
     2041 tcgacaccta ctaccgccgg acggacgtgc ccgcctcgct ggtgcgcgcc gaccgcgccc
     2101 gcgccttcga gacgtacgac acgatggacg tgcagctctc gggcgtgaag atcaacctcg
     2161 actcgtcggg cacgcgcgcg accgtcaccc tcgacaagac ttgggaattc gcgtcggaag
     2221 acaaagactc gaacggctcc gtgcgccagc agctcacgct ctcgaagatc gacgggcgct
     2281 ggctcatcac cggcgagaaa gacctcgaag tccactactc gaacagcgtg aagaactagg
     2341 cgggcggcgc gacgaaccgg cgcggctcta ttttcgaggt gaaccggagt ttgagcgaaa
     2401 cgaccttcaa cctgcggcgg gccgcggcgg gcgaccgtga agtgctcgtc gagttcaacc
     2461 tcgcgatggc gcgcgagacc gaggcgaagg agctttcgcc cgaagtcttg cgcgcgggcg
     2521 tcgcgcactt catcgagagg ccgcagtacg gcttctacgt cgtcgccgaa gacgggggcc
     2581 aagtggtcgg ctcgctcatg atcacctacg agtggagcga ctggcgcgac ggcctcttct
     2641 ggtggattca gagcgtctac gtccgcccct ccgcccgccg ccgcggcgtc taccgccgcc
     2701 tctacgaaca cgtcaaacgc ctcgccgccg cggacgacag cgaagtccga ggcttccgcc
     2761 tctacgtcga gaaggagaac cgcgtcgcgc aggcgaccta cgaacagctc ggcatgtccg
     2821 agacgcacta cctgatgtac gag
//
LOCUS       DBNGOH010001093         3275 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2914856, whole genome shotgun sequence.
ACCESSION   DBNGOH010001093 DBNGOH010000000
VERSION     DBNGOH010001093.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3275)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3275)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3275
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2914856"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..575)
                     /locus_tag="AC159P_21315"
     CDS             complement(<1..575)
                     /locus_tag="AC159P_21315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3698042.1"
                     /translation="MAKSEAEVIPIRPARTYRHKVLAHAAERLAAGGPLPQTSEISEQ
                     FRAFLKVEDRRLKMALRLGASGSETAAARSFVLDLVAERAFQTATLDGEGGVFVGAAV
                     ESCAMVAVGGYGRAELAPHSDLDILFLHTGRRSTQMRQLVERVLRLLWDAGLTVGHSF
                     RTVSECTSAARTDPHLQTSLLSTRQLAGNGAL"
     gene            complement(699..923)
                     /locus_tag="AC159P_21320"
     CDS             complement(699..923)
                     /locus_tag="AC159P_21320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3698043.1"
                     /translation="MALILKGQEFVATEALSARLLSHLAALAARRTLTLNGIKMCAQD
                     LAKLAEEAGRLNRPLWPVVPAGCAACYPND"
     gene            1697..2443
                     /locus_tag="AC159P_21325"
     CDS             1697..2443
                     /locus_tag="AC159P_21325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3698044.1"
                     /translation="MEQKYIMLRAKSIAGAGLFVLALALAGAAEASAQSTLFNIPSTD
                     VVAEKKLYLEFDYVGHLEKFEDGGFHAYVPRAVVGVGKNVEVGVNVPVNHLDSLHPVE
                     VQPNIKWKFYNDEDSGVAAAAGAIMYVPVHEKAAFANDDFGLLYSVVSKKVKGTYGPR
                     ITGGGYGLVGRADGLGTKGGAIVGYEQPLHRRVNFVADWLSGKNRFGYVTPGFSFTVS
                     SRSALYAGYSIGNQGRGNNALFVYYGITLF"
     gene            2645..>3275
                     /locus_tag="AC159P_21330"
     CDS             2645..>3275
                     /locus_tag="AC159P_21330"
                     /inference="COORDINATES: protein motif:HMM:NF012348.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:1901704 - L-glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase beta-grasp
                     domain-containing protein"
                     /protein_id="HIR3698045.1"
                     /translation="MSTNAALKFAADEKAAYVNVRFTDLVGAWHHLTFPVEELSEKSF
                     EEGFGFDGSSLRGWASIHESDMLLVPDATRFWIDPFMSEKTVCFVADVVDPITKEGYW
                     LDPRGVGRRAESYLKFTGIADTVYFGPEAEFFVFNAVSFHNDPHTAGFSLDNPEASWN
                     SNNTNGNSNLGYHIRNKEGYVPLPPLDTLQDFRTEVATELKKVGIHVECH"
BASE COUNT          604 a         1062 c         1039 g          570 t
ORIGIN      
        1 agcgcgccgt tgcccgccag ctgccgcgtc gagagcagag acgtttgcag gtgcgggtcg
       61 gtgcgcgccg ccgaggtgca ttcggagacg gtgcggaagc tgtggccgac cgtgaggcct
      121 gcgtcccaga gcaggcgcag gacgcgctcg acgagctgac gcatctgcgt cgagcggcgg
      181 cccgtgtgta gaaaaagaat gtcgaggtcg gagtgcggcg ccagctccgc gcgcccgtag
      241 ccgccgacgg cgaccatcgc gcaactttcc accgccgcgc cgacgaagac gccgccctct
      301 ccgtcgagcg tggcggtctg gaaggcgcgc tcggccacga ggtcgagcac gaagctgcgc
      361 gcggcagccg tctcgctgcc cgacgcgccg agcctgagcg ccatcttcag acgacggtct
      421 tcgaccttga ggaaggcgcg gaactgctcg gatatctcgc tcgtctgcgg caacgggccg
      481 cccgcggcga gcctctcggc ggcgtgcgcc agcaccttgt ggcggtatgt gcgcgccggt
      541 cggatgggga taacctctgc ctcgctcttc gccataaaac taaagtgctg agtactgagt
      601 actgagtact gagttaaaga caaactgctt tcactcagta ctcagtactc agtactcagc
      661 actttgtttt tagtccgcag tcctcagtac tttcattatt agtcgttcgg gtagcaggcg
      721 gcgcagccag ccgggacgac gggccacagc gggcggttca aacgccccgc ctcctcggcg
      781 agcttcgcca agtcctgcgc gcacattttg atcccgttca gcgtcagcgt gcggcgcgcg
      841 gccagcgccg ccagatgcga taggaggcgc gcgctgagcg cctcggtcgc gacgaattcc
      901 tgccctttca gaatgagtgc catgcagctc gctcgccccc tttcgtcagc agtcgggagg
      961 ggtgacaacc ccgcccgcgc ccctcgattg tgaattgatg accgactctc acgattccgt
     1021 tcagcaacaa tcattccgcg ctcacgctcg cgcagcagag tcgagcggcg cggagtagtt
     1081 tgcagagttt tcgccctcat cttcaaaccg cgcgggccgt cgccctgcga catttttgtc
     1141 gcgcacaccg gcgggcgatt caaaagtgta atgcggagtt cgaatgatcg gttcggcgtt
     1201 atcacgccct tcagttcaag cggcgcgcct ctgcctacct ccgcgtcgaa acgctatcga
     1261 cttcatgccc gacgaccgcc ctcgaacgac gaccgtcctc acacgggccg accgacgaag
     1321 ggcgtgctga ttgacgagtc ggaaggcggt ctggtcgtgc gacaaatttg tcgtgggccg
     1381 cgcggcgtcc aacgaaaatg tcgcgcgcgg ggtggcggaa gggaactgta tcgggagggg
     1441 cgcgcggcgg gcggaaagtg taaccagccg tatatcaacg cttcggcgcg cggggcgcgg
     1501 gggcggtcgg cgggtggcac acaatttgtg aagcggagtg cgaaatgtaa agggtgagga
     1561 gcgcgcggcg cttccttgca gcacggtctc catttcacat gaggcttaac ggaaacaaca
     1621 tggcccgcac gtctgaagct tgcaggcgcc aaaacgcgcg ggccactgaa gtcgtccctt
     1681 ttgcaaggag acgattatgg agcaaaagta catcatgctc agagcgaaat caatcgcggg
     1741 ggcgggcctc ttcgtgctcg ccttggcact cgcgggcgcg gcggaggcga gcgcgcagtc
     1801 caccctcttc aacatcccct cgaccgacgt ggtggcggag aagaaactct acctggaatt
     1861 cgactacgtc ggccaccttg agaagttcga agacggcggg ttccacgcct acgtcccgcg
     1921 cgcggtggtc ggggtcggca agaacgtcga ggtcggggtg aacgtccccg tcaaccacct
     1981 cgactcgctg cacccggtcg aagtccagcc gaacatcaag tggaagtttt acaacgacga
     2041 ggacagcggc gtggccgccg ccgcgggcgc catcatgtac gtccccgtcc acgaaaaggc
     2101 ggcgttcgcc aacgacgact tcgggctgct ctactccgtc gtcagcaaga aagtgaaagg
     2161 cacgtacggg ccgcggatca ccggcggcgg ctacgggctg gtcggtcgcg ctgacgggct
     2221 ggggacgaag ggcggggcca tcgtcggcta cgagcagccg ctccacagga gggtcaactt
     2281 cgtcgccgac tggctgagcg gcaagaaccg cttcggctac gtgacgccgg gcttctcttt
     2341 caccgtttcc agccggagcg cgctctacgc cggctacagc atcggcaacc aggggcgcgg
     2401 gaacaacgcg ctgttcgtct actacggcat caccctgttc tgacgggaaa ggaggtgccc
     2461 ggtcgagagt ctgattagac aagctgtttc gccgtcgtag ctttcaaaac aggccgcgag
     2521 cggtcggcgc gcgcgccccg gttgcgcgga cgccgaccgc ccgtggccgc cgtggcgcgg
     2581 tcggcccgcc gaggccgccc cacgtttctg aacaccccta acatctctta aggaaaaatt
     2641 attcatgagc accaatgcag cattgaagtt cgccgccgac gagaaggcgg cctatgtcaa
     2701 cgtccgtttc acggacctcg tgggcgcgtg gcaccacctg accttcccgg tcgaagagct
     2761 ttcggaaaaa tctttcgaag agggcttcgg cttcgacggc tcctctctgc gcggctgggc
     2821 ctcgatccac gagtccgaca tgctgctcgt gcccgacgcg acgcgcttct ggatcgaccc
     2881 gttcatgtcg gagaaaaccg tctgcttcgt cgccgacgtg gttgacccga tcaccaaaga
     2941 gggttactgg ctcgacccgc gcggcgtggg ccgccgcgcc gagagctatc tcaagttcac
     3001 gggcatcgcc gacaccgtct acttcggccc cgaggctgag ttcttcgtct tcaacgccgt
     3061 cagcttccac aacgacccgc acaccgcggg cttctcgctc gacaaccccg aggcgtcttg
     3121 gaactcgaac aacacgaacg gcaactcgaa cctcggctac cacatccgca acaaggaggg
     3181 ctacgtccct ctgcccccgc tcgacacgct gcaagacttc cgcacggagg tcgccaccga
     3241 gctgaagaag gtcggcatcc acgtcgagtg tcacc
//
LOCUS       DBNGOH010001094         6140 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3282330, whole genome shotgun sequence.
ACCESSION   DBNGOH010001094 DBNGOH010000000
VERSION     DBNGOH010001094.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6140)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6140)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6140
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3282330"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..678)
                     /locus_tag="AC159P_21335"
     CDS             complement(<1..678)
                     /locus_tag="AC159P_21335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006103514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="von Willebrand factor type A domain-containing
                     protein"
                     /protein_id="HIR3698046.1"
                     /translation="MKKPASKTSRLSWLRGRFASKNDTRFLISLVLAACVLAASCARS
                     NKQGGEADGPIGVAENRSGQPSASANPSGTFGRQIVDEGEEQAKRRKGESYGVIDENP
                     FLEAARAPLSTFSIDVDTASYSNVRRFLNDGQLPPKDAVRVEELINYFSYDYPQPAGA
                     TPFSVTTEVADCPWDTRHRLVHIGLQGRRVASENLPPANLVFLVDVSGSMQPANRLPL
                     VKTSLRML"
     gene            complement(832..1401)
                     /locus_tag="AC159P_21340"
     CDS             complement(832..1401)
                     /locus_tag="AC159P_21340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3698047.1"
                     /translation="MKKLAGVAVFVVTLVVALLVTRYYAPRPEPPPEPPIAPIAADPP
                     LFPEAPPPPSRSKVNYKVQLVTLDVAARKSHATLVLERAPDAPAPERVWVWAGFFVPG
                     DAGGEIFAGAPVLVEKPFAGGATRATFNVTGACPWCGQGDAPAAGYYARVNVSDESAA
                     DAQLEDEEVSRDLSTATQVVVEEGRRRSR"
     gene            complement(1542..3140)
                     /locus_tag="AC159P_21345"
     CDS             complement(1542..3140)
                     /locus_tag="AC159P_21345"
                     /inference="COORDINATES: protein motif:HMM:NF016800.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008754 - O-antigen ligase activity
                     [Evidence IEA];
                     GO_function: GO:0008755 - O antigen polymerase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HIR3698048.1"
                     /translation="MKREKGKEEKTETLFAASPFSFFASSSLDRALVGCLFLLALSAP
                     HSIAVSQGAWLLSMLLWVVRLFVRPRPPLWRTPVDVALLGFFTLTFLTALTSYDPEVS
                     IGKLRAASLFTVVYVVAENVRGRRVLRGLALALVASCALNVVFTFGALAVGRGVKARE
                     LRQDGPLYAAGLREGDTVLAAEGLPVETPEELERAIGDASRAGQVTMRWESNKPACVS
                     TEREACVRAYRSEALLPLLVVRDTALAGGTAAERLGLARWTKGRDERARGFFGHYTTY
                     AEALQLIASLALGMFVALGRKWSMKGVLLGAALAGMCGALLLTVTRASWLGLLLSALT
                     IAAVGARRRTLLIVAAIALPLAVAGLFVLQQKRRVGFLDAKDGSTAWRLMVYREALDL
                     LVREPRHAAVGVGMDSIKRHYREWGLFDKGRQQWSHLHSTPLQLAVERGLLTLFAWLA
                     LVFLYARMLWRMARTGSGVDWLTRGLALGALGGLVGFLTSGLVHYNWGDSEVIMIFYF
                     VMGLALAAERPARAEEREGAVREG"
     gene            complement(3166..3507)
                     /locus_tag="AC159P_21350"
     CDS             complement(3166..3507)
                     /locus_tag="AC159P_21350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006297844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6176 family protein"
                     /protein_id="HIR3698049.1"
                     /translation="MYETTCMKIKLHPGSLERVREWAAEINRRSDEALATLRDERVVV
                     ESVFLESGAEGDFLIYYMKGASIEGAREVADNSKHAIDEYHRRFMREVCASGQKLELL
                     VDLDRIGEADE"
     gene            complement(3586..4449)
                     /locus_tag="AC159P_21355"
     CDS             complement(3586..4449)
                     /locus_tag="AC159P_21355"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015932935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3698050.1"
                     /translation="MNVQLRPATPADAEECGRIIYEAFSGIADRHNFRRDFESPEFAT
                     QLAAAFIANPSVFGVVAEQEGRVVGSNFLWEHDPIRAVGPVTVDPATQARGVGRVLMN
                     AVIERGRGAAGVRLVQDAFNLASLSLYTSLGFDVREPLVEIEGRPTAGPPDGYEVRPL
                     KDEDLGACEELCRRVHGFERTNELKATPPMLTPVVALRGGRVAAYASGPTFWAMNHAV
                     AETDEDMQALLAGAAGASEAPLSFILPTRQAGLFRWCLKNGLRVVKPMNLMSTGEYHE
                     PRGCYLPSVGY"
     gene            complement(4705..>6140)
                     /locus_tag="AC159P_21360"
     CDS             complement(4705..>6140)
                     /locus_tag="AC159P_21360"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HIR3698051.1"
                     /translation="VACLVGAYLLREGGGVLVRFTLAEGFVWHPPFEPYGALLLLVVP
                     IRMLALLYYKLYSLRGEFSYVEDVARVFKATAVGSLLIVAAAFLYRGGYEFRAFSYAR
                     GVFVLDFALALAAYSLLRLALRGAQSAVRRREVNLIPTLVVGRNHEAALCVREMMERR
                     ELGYRVIGVVETGALAAAPAEFEGVPVICGFAELSDAIRETGANEVIITDPAISGDLL
                     FDVMMRVGRRRGVEFRLAPSLFNCLPRKTAVDQIGALPMIRLFREPLPRAARAAKRAF
                     DIFVAGLALVLLAPVWAVVAALIKLGSRGPVFYKQERVGMDGRVFLFYKFRTMRAGSD
                     DRAHREYQQKYIAGLPDTNLGEGHRPVYKMRDDPRITRVGRLLRRLSLDELPQLWNVL
                     AGDMSVVGPRPPIPYEVEAYELWHRKRLDMKPGLTGLWQVSGRNRLTFEEMVKLDLFY
                     IENWSPLLDLKIIFRTLPVMLRGDDAY"
BASE COUNT         1028 a         2177 c         2023 g          912 t
ORIGIN      
        1 cagcatcctg agagaggtct tgacgagcgg gagcctgttc gcgggctgca tcgagccgga
       61 cacgtccacg aggaagacga ggttggcggg cggcagattc tcgctcgcca cgcggcggcc
      121 ttgcagcccg atgtggacga ggcggtgccg cgtgtcccag gggcagtcgg ccacctcggt
      181 cgtcacggag aagggcgtgg cgcccgcggg ctgcgggtag tcgtaggaga aatagttgat
      241 gagttcctcg acgcggacgg cgtccttcgg cggcaactgg ccgtcgttga gaaagcggcg
      301 gacgttgctg taagaggctg tgtccacgtc gatcgagaag gtcgagagcg gcgcgcgcgc
      361 ggcttcgagg aaggggttct cgtcgatcac gccgtacgat tcgcccttcc tgcgcttcgc
      421 ctgctcttcg ccctcgtcaa cgatctggcg cccgaaggtc cctgacgggt tcgcgctcgc
      481 gctcggctgg cccgacctgt tttccgccac cccgataggt ccgtccgcct cgccgccctg
      541 cttgttactc ctcgcgcacg acgcggccag cacgcaggcg gcgaggacga gcgagatcaa
      601 aaagcgtgtg tcgttcttcg aggcgaagcg accgcgcagc catgagaggc ggcttgtttt
      661 cgatgcaggc ttcttcatgg agagttcctt tccgcgaggc ccattcttga caccgaccga
      721 cgacacttga atgcgcccgg tgccggaatc ttgcgcgccc gaacggcgcg cgggccgaag
      781 ggtcggactt gacggttaac gcggcgagga aagcatgacg aaaagctcgc ttcagcgact
      841 ccggcggcgg ccctcttcga ccacgacctg cgtcgccgtc gagaggtcgc ggctcacctc
      901 ttcgtcttcg agctgcgcgt ccgccgccga ctcgtccgag acgttgacgc gcgcgtagta
      961 gcccgcggcg ggcgcgtcgc cctgtccgca ccaggggcac gcgcccgtga cgttgaaggt
     1021 cgcgcgcgtc gcgccccccg cgaaaggctt ctcgacaagg acgggcgcgc ccgcgaagat
     1081 ttcgccgccc gcgtcgcccg gcacgaagaa gccggcccac acccacaccc tctcgggcgc
     1141 gggcgcgtcc ggcgcgcgct ccagcacgag cgtcgcgtgg ctcttgcgcg cggccacgtc
     1201 gagcgtgacg agctgaactt tgtaattgac tttcgagcgc gacggcggcg gcggcgcctc
     1261 cggaaacagc ggcgggtcag ccgcgatggg cgcgatgggc ggctccggcg gcggctcggg
     1321 ccgcggcgcg tagtaacgcg tgacgaggag cgccacgacg agcgtgacga cgaagaccgc
     1381 aacgcctgcc agtttcttca taacccgctt cctctccggt cgctttccgt ccccggacga
     1441 tcccacgcgg aaagacgcga gcggcgcggc ccatgaaatc aaagcccgcc gcccctgtct
     1501 ccgtggctaa ccacacttcc cgacgggcga acgctcgcgc ctcagccttc ccgcaccgcg
     1561 ccctcgcgct cttccgcgcg cgccggcctc tcggcggcga gcgccagccc catgacgaag
     1621 taaaagatca tgatcacttc cgagtcgccc caattgtaat gcaccagccc gctcgtcagg
     1681 aatcccacca gcccgccgag cgcgccgagc gccagcccgc gcgtgagcca atcgacgccc
     1741 gaccccgtgc gcgccatccg ccacagcatc cgcgcgtaga ggaagacgag cgcgagccac
     1801 gcgaagagcg tcaagaggcc gcgctcgacc gcgagttgca gcggcgtcga atgcaggtgg
     1861 ctccactgct gccgcccctt gtcgaacagc ccccactcgc gatagtgccg cttgatcgag
     1921 tccatgccga cgccgaccgc ggcgtgccgc ggctctctca ccagcaagtc cagcgcctcg
     1981 cggtagacca tcaaacgcca cgccgtcgag ccgtccttcg cgtcgaggaa acccacgcgc
     2041 cgcttctgct gcaggacgaa cagccccgcg accgcgagcg gcagcgcgat tgccgcgacg
     2101 atcagcaaag tgcgacgacg cgcgcccacc gccgcgatgg tcagcgccga cagcagcagc
     2161 ccgagccaag aggcgcgcgt caccgtcaac aacagcgcgc cgcacatccc cgccagcgcc
     2221 gcccccaaca gcacgccctt catgctccat ttgcgcccga gcgccacgaa catcccgagc
     2281 gccagcgacg cgatcagttg cagcgcctcg gcgtacgtcg tgtagtggcc gaagaagccg
     2341 cgcgcgcgct cgtcgcgccc cttcgtccag cgcgcgaggc cgagcctctc cgccgccgtc
     2401 ccgcccgcga gcgccgtatc gcgcacgacg agcaaaggca acagcgcctc cgagcggtac
     2461 gcgcgcacgc acgcctctcg ctccgtcgag acgcacgcgg gcttgttgct ctcccaacgc
     2521 atcgtcacct gcccggccct gctcgcgtcg ccgatggcgc gctccaactc ttccggcgtc
     2581 tcgacgggca agccttccgc cgccagcacg gtgtcgccct cgcgcaagcc cgccgcgtag
     2641 agcggcccgt cctgccgcag ctccctcgcc ttcacgcccc tgccgacggc gagcgcgccg
     2701 aacgtgaaca cgacgttcaa cgcgcacgac gcgacgagcg ccaacgccag cccgcgcagc
     2761 acgcgacgcc cgcgcacgtt ctccgccacc acgtagacga ccgtgaagag gctcgccgcg
     2821 cgcagcttcc cgatggacac ttccgggtcg taagaggtga gcgccgtcag gaaggtcagc
     2881 gtgaagaacc cgagcagcgc cacgtcaacc ggcgtgcgcc acaacggcgg gcgcggtcgc
     2941 acgaagagcc tgacgaccca cagcagcatc gacagcagcc acgcgccctg cgacaccgca
     3001 atcgaatgcg gcgcgctcaa cgccagcagg aacaggcacc cgacgagcgc gcggtcgagg
     3061 gaagaagagg cgaaaaagga aaagggggaa gcggcgaaga gggtttcggt cttctcttcc
     3121 ttcccttttt ccctcttcac ctcttcccct ttcaccccca aagcattact catccgcttc
     3181 cccgatgcgg tcgagatcga ccagcagttc gagcttctgc cccgacgcgc agacctcgcg
     3241 catgaaccgt cggtgatact cgtcaatcgc gtgcttcgag ttgtccgcca cttcgcgcgc
     3301 cccctctatg ctcgcgccct tcatgtagta aatcagaaag tcgccctcgg cgccgctctc
     3361 aaggaacacg gactcgacca cgacgcgctc gtcgcgcaag gtcgccaacg cctcgtcgct
     3421 ccggcggttg atctccgccg cccactcgcg cacgcgttcc agcgagccgg ggtgcagctt
     3481 gatcttcatg caggtcgtct cgtacatctt cactcctcta tagagatgtc ggaagtcgga
     3541 agtcggaaga agaagcggct ttttattccg acttccgact tctcttcagt atccgaccga
     3601 cggcaggtaa cagccgcgcg gctcgtggta ttcgccggtg gacatcaggt tcatcggctt
     3661 gacgacgcgc aggccgttct tgaggcacca gcggaagagg ccggcctgcc gcgtcgggag
     3721 gatgaaggac aggggcgctt cgctcgcacc ggcggcgccc gcgagcagcg cctgcatgtc
     3781 ttcgtcggtc tcggcgacgg cgtggttcat cgcccagaag gtcggccccg aggcgtaggc
     3841 cgcgacgcgc cccccgcgca gggcgacgac gggcgtcagc atcggcggcg tggccttcag
     3901 ctcgttcgtg cgctcgaagc cgtggacgcg ccggcacagc tcctcgcagg cgccgaggtc
     3961 ttcgtccttc agcggtcgca cttcgtaacc gtcgggcggc cccgccgtgg gtctgccctc
     4021 gatctcgacc agcggctctc tcacgtcgaa gccgagcgag gtgtagagcg agagcgaggc
     4081 gaggttgaac gcgtcctgca cgaggcggac tccggcggcg ccgcgtccgc gctcgatgac
     4141 ggcgttcatc aggacgcgcc cgacgccgcg cgcctgcgtc gcagggtcaa ccgtgacggg
     4201 gccgacggcg cggatcgggt cgtgctccca gaggaagttc gagccgacga cgcggccctc
     4261 ctgttcggcg acgacgccga agaccgaagg gttggcgatg aaggccgcgg cgagctgcgt
     4321 cgcgaattcc ggcgattcga aatcgcggcg gaagttgtgc cggtcggcga tgccgctgaa
     4381 cgcttcgtag atgatgcgcc cgcactcttc ggcgtcggcg ggcgtggcgg ggcggagttg
     4441 tacgttcatg attttttttc ggcttaagtc ctgagtatgc gtgcgaagtc cggcactcgt
     4501 gagcgtgacc ctccgccgcg tagcggcgct tgattgtagc ccggcgtttc aacgccgggt
     4561 gagatttgtc aacgcagtcc ctcgtcgcgt cagcgacgac tgagcgaagg ttcaagcgtc
     4621 gctagcgcga cgccgggtga cgtatatatc gactgccccg gccttgaaag gccgagctac
     4681 attcagacgc cgctccgcgg cgcgttagta cgcgtcgtcg ccgcggagca tgactggtaa
     4741 agtgcggaag atgatcttga ggtcgaggag cggcgaccag ttttcgatgt agaagaggtc
     4801 gagcttgacc atctcttcga aagtcaggcg gttccggccc gagacctgcc agagacccgt
     4861 gaggcccggc ttcatgtcga gccgcttgcg gtgccacagc tcgtaggctt cgacctcgta
     4921 agggatcggc gggcgcgggc cgacgacgct catgtcgccc gcgagcacgt tccacagttg
     4981 cggcagctcg tccagactca gccggcgcag cagccgcccg acgcgcgtga tgcgcgggtc
     5041 gtcgcgcatc ttgtagacgg gccggtggcc ttcgccgagg ttcgtgtccg gcagccccgc
     5101 gatgtacttc tgctggtact cgcggtgcgc gcggtcgtcc gagccggcgc gcatcgtgcg
     5161 gaacttgtag aagaggaaga cgcgcccgtc catcccgacg cgctcctgct tgtagaagac
     5221 cggcccgcgc gagccgagct tgatgagcgc ggcgaccacg gcccacaccg gcgcgagcag
     5281 gacgagcgcc agtccggcga cgaagatgtc gaacgcgcgc ttggcggcgc gcgcggcgcg
     5341 cggcagcggc tcgcggaaga ggcggatcat cggcagcgcg ccgatctgat ccacggcggt
     5401 cttgcgcggc aggcagttga agaggctcgg cgcgaggcgg aactcgacgc cgcgcctccg
     5461 gcccacgcgc atcatcacgt cgaacaggag gtcgccggag atggccgggt cggtgatgat
     5521 gacctcgttc gcgcccgtct cgcggatggc gtccgacagc tcggcgaagc cgcagatgac
     5581 gggcacgccc tcgaactccg ccggggccgc ggcgagcgcg cccgtctcga cgacgccgat
     5641 gaccctataa cccagctcgc gccgctccat catctcgcgc acgcacagcg ccgcctcgtg
     5701 gttgcgcccg acgacgagcg tcgggatgag attcacctcg cgtctgcgga cggcgctctg
     5761 cgcgccgcgc agcgcgagcc tgagcagcga gtaggccgcg agcgcgagcg cgaagtcgag
     5821 cacgaagacg ccgcgcgcgt aagagaaggc gcggaactcg tagccgccgc ggtagaggaa
     5881 cgccgccgcc acgatgagca gcgagccgac cgccgtcgcc ttgaagacgc gcgccacgtc
     5941 ctccacgtaa gagaactcgc cgcgcaggct gtagagcttg tagtacaaca gcgccagcat
     6001 gcggatcggc acgacgagca gcagcagcgc cccgtaaggc tcgaacggcg ggtgccacac
     6061 gaacccctcg gcgagcgtga accgcacgag caccccgccg ccctcgcgca gcagatacgc
     6121 gccgaccaga cacgcgaccg
//
LOCUS       DBNGOH010001095         2918 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1632227, whole genome shotgun sequence.
ACCESSION   DBNGOH010001095 DBNGOH010000000
VERSION     DBNGOH010001095.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2918)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2918)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2918
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1632227"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1996
                     /locus_tag="AC159P_21365"
     CDS             <1..1996
                     /locus_tag="AC159P_21365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3698052.1"
                     /translation="VRESGVYQWQTGRTNTARWDAYAQYWAFNYILYANSPRYFRREG
                     TAAWRCRASYQPGDDEPGAREEAFPELWERIPRGLLHLISESDCLPVHHFAVKALRAC
                     EQFNAELEREALLMILGRPYEVTAHFGYELVRARLRAAAPDQALVLAVANCASPEARA
                     EAQRWIDEGREHFLRDGDFAIALVTSPRPDTREFARRLLRSTALPDTEARALVARLIA
                     YLTGLDAARGEEAGDIAATVLDCFGKQLRTLGLGVVLDLLTHPLAEVQELGGNILLLH
                     EVRPSELPEQIIISLIDSPHEPLRGIGVRLLGELPDQSLLEREGLLVALARHPLADLR
                     NAVRPVIRRLSHNAAAADFTRRLARLLLATLLAPEAVEGVHRSVAQLLQEDLGGAWAD
                     DVDKDSALRLARAASPAARELGGSLIDTRAQADAAWAQSFTTDEIVELADAEVFAVRQ
                     AARTLFGLVTDRYRQATNPGGHLDEVARAVRLLDTRWEDARAFFFAVFRTRLGAADFT
                     PGILVSVCDSVRAEVQQFGRELVTKYFSEEAGQEYMLKLSEHPSAELQLFVTNYLERY
                     CAGDPQRLREMSHYFTSVLSRVNKARVAKARVIAFLTSEARKSEEEARVVAEILARQS
                     ATVAVGMKASAIEAMLIIRRAYPDIPLPLDIKPAEVRRAV"
     gene            1986..>2918
                     /locus_tag="AC159P_21370"
     CDS             1986..>2918
                     /locus_tag="AC159P_21370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017719897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HIR3698053.1"
                     /translation="MLFEYTYRGASAVDSTADRTLMSFAPDVTRDPTFFTGRLRRRIE
                     FREAISALHDVVVSDLRFKPKDKTAYKEWAARQEQIDWQTIAAERADVAGRIGELQAE
                     LDDLRARSVHRMGPFNKARRGYQNYILKTEKDFWRVLFDPVITVHPDEVFFECFSQDE
                     SSYGRLGAGYEVFEQIGEFACGTTNIDYSAALYDEFQKIRTYKETRFEIDPSGFDVRT
                     TGEAAYKEVKIDLPDSWVRGFLQVSSAMSLPARTFDLDPMDIHNICFVLRRRRERQGP
                     RAMRYHLTPGEPVRVVFEPWETEVVCARSVYAGAD"
BASE COUNT          488 a         1002 c          971 g          457 t
ORIGIN      
        1 cgtgcgcgag tcgggcgttt accagtggca gacgggccgc accaacacgg cgcgctggga
       61 cgcctacgcc cagtactggg cgttcaatta catcctctac gccaacagcc cgcgctactt
      121 ccgccgggag ggcacggcgg catggcgctg ccgcgcgtct tatcagcccg gcgacgacga
      181 gccgggggcg cgcgaggaag ccttccccga gctgtgggag cggattccga ggggcctgct
      241 gcacctcatc tccgaaagcg actgcctccc tgtccaccat ttcgcggtca aggcgctccg
      301 ggcctgcgaa cagttcaacg ccgaactcga acgcgaggcg ctcttgatga tcctcggtcg
      361 cccgtacgag gtgacggcgc acttcggcta cgaactggtc agagcgcgcc tccgcgcggc
      421 ggcgcccgat caagcgctcg tcctcgccgt cgccaactgc gcctcgccgg aggcgcgcgc
      481 cgaagcgcaa cgctggatcg acgagggacg cgaacacttc ctgcgtgacg gcgacttcgc
      541 aatcgcgctg gtcacgagtc cccgacccga cacgcgcgag ttcgcgcgca ggctgttgcg
      601 ttcgaccgcg ctcccggaca cggaggcgcg cgcactcgtc gcccggctca tcgcttacct
      661 gaccggactg gacgcggcgc ggggcgagga ggcaggcgac atcgccgcga ccgtgctgga
      721 ctgtttcggg aagcagttgc gcacgctcgg cctcggcgtc gtcctcgacc tgctcacaca
      781 ccccctcgcc gaggtgcagg agctgggcgg caacatcctg ctgctgcacg aggtgaggcc
      841 gagcgagctg ccggagcaga tcatcatctc tctgatcgac tcgccgcacg agcctttgcg
      901 cggcatcggc gtgagattgc tcggcgagct tccggaccag tcgctgctcg aacgggaggg
      961 actgctcgtc gcgctcgccc ggcacccgct ggccgacctg cgcaacgccg tccgccccgt
     1021 catccgccgc ctcagtcaca acgccgctgc cgccgatttc acgcgcagac tcgcgcgact
     1081 actcttagcg acactcctcg cacccgaagc cgtcgagggc gtgcaccgga gcgtcgcgca
     1141 gttgctgcaa gaggacttgg gcggcgcgtg ggccgacgac gtcgataagg attcggctct
     1201 ccggttggcg cgcgcggctt ctcccgcggc gcgcgagttg ggagggagcc tgatcgatac
     1261 cagggcgcag gcggacgcgg cgtgggcgca gtcattcacg acggacgaga tcgtcgaatt
     1321 ggccgacgca gaagtgttcg cggtgcggca agctgcgcgg acgctcttcg gcctggtgac
     1381 ggaccgttac cggcaggcca ccaaccccgg cggccatctg gacgaggtgg cgcgggcggt
     1441 tcgcctgctc gacacgcgtt gggaagatgc gcgcgccttc ttcttcgcgg tcttccgcac
     1501 gcggctcggc gcggcggact tcacgccggg catactggtc agcgtctgcg acagcgtgcg
     1561 cgccgaggtg cagcagttcg gacgagaact ggtgacgaaa tatttcagcg aggaggccgg
     1621 acaggagtac atgctcaagc tgagcgagca cccgtcggcg gagctgcaac tcttcgtcac
     1681 caactacctc gaacgctact gcgccggcga cccgcagcgc ctgcgcgaaa tgtcccacta
     1741 cttcacgagc gtgctctcgc gcgtgaacaa ggcgcgcgtt gccaaagcgc gagtcatcgc
     1801 gttcctcacg tccgaggcgc ggaagagcga ggaggaggcg cgcgtcgtcg ccgaaattct
     1861 cgcgcgccag tcggcgaccg tggcggtcgg gatgaaagcc tccgccatcg aggccatgct
     1921 catcatccgc cgcgcgtacc cggacatccc cctcccgctc gacatcaaac ccgcggaggt
     1981 gcgccgtgct gtttgagtac acctaccgag gcgccagcgc cgtcgacagc acagccgacc
     2041 ggacgctcat gtcgttcgcg cccgacgtga cgcgcgaccc gaccttcttc acgggcaggc
     2101 tgcgccggcg catcgagttc cgcgaggcga tcagcgcgct gcacgacgtg gtcgtctccg
     2161 acctccggtt caagccgaaa gacaagaccg cttataagga gtgggccgcg aggcaggagc
     2221 agatcgattg gcagactatc gccgccgagc gcgccgatgt cgccgggcgg atcggagagt
     2281 tgcaggccga gcttgacgac ctccgcgcgc gcagcgtgca ccgcatgggg cctttcaaca
     2341 aggcccgaag gggttatcaa aactacatcc ttaaaacgga gaaagatttc tggcgcgtgc
     2401 tcttcgaccc cgtcatcacc gtccaccccg acgaagtctt cttcgagtgc ttcagtcagg
     2461 acgagtcgag ttacgggcgg ctcggcgcgg gctacgaggt gttcgagcag atcggcgagt
     2521 tcgcctgcgg cacgacgaac atcgactact cggcggcgct ctacgacgag tttcagaaga
     2581 tacgcaccta caaagagacc cgcttcgaga tcgacccctc cggcttcgac gtgcggacga
     2641 ccggcgaggc cgcctacaag gaggtgaaga tcgacctccc cgatagctgg gtgcgcggct
     2701 tccttcaggt cagctcggcg atgtcgctgc ccgcgcgcac gttcgacctc gacccgatgg
     2761 acatccacaa catctgcttc gtgctgcgcc gccgccgcga gcggcagggg ccgcgtgcga
     2821 tgcgctatca cctcacgccg ggcgagccgg tcagggtggt ctttgagccg tgggagacgg
     2881 aggtcgtgtg cgcccgctca gtctacgcgg gcgcggac
//
LOCUS       DBNGOH010001096         3264 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2915212, whole genome shotgun sequence.
ACCESSION   DBNGOH010001096 DBNGOH010000000
VERSION     DBNGOH010001096.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3264)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3264)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3264
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2915212"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..220)
                     /locus_tag="AC159P_21375"
     CDS             complement(<1..220)
                     /locus_tag="AC159P_21375"
                     /inference="COORDINATES: protein motif:HMM:NF012698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HIR3698054.1"
                     /translation="MNGPKNHIYEFGDFRIDVGRRLLLRSSGDPVPLTPKVFDTLLYL
                     VEHSGSVLDKTALMDAIWPDTEVEENNLS"
     gene            346..849
                     /locus_tag="AC159P_21380"
     CDS             346..849
                     /locus_tag="AC159P_21380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3698055.1"
                     /translation="MKLRTVRSMFGAALTLILALTATQPSASAQGESGKLSSGNGKIV
                     GVWDVRVTRRDCQTGDALGVNPPVMNMFNSGGTLLETSSGLPTRRGPGLGTWQYVGGG
                     SYSAVFRFFRFNADGSFAGTRRVARTIELSQDANEFTATATDELFDASGNLFQAGCAT
                     ETATRLE"
     gene            1080..2315
                     /locus_tag="AC159P_21385"
     CDS             1080..2315
                     /locus_tag="AC159P_21385"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014081.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR3698056.1"
                     /translation="MKLRLVLTLLLLLLNAAGHVPAPRAQTPATPARLVIRNATLIDG
                     LSPRLVHGATVVVSDGKIESVSSGPVEVPAGATVLDLKGRWLLPGLIDAHVHFVDPGA
                     ARTALASGVTTARSLGAERFSDVGIRELNHEGVADLPDVVASGYQVTSQVGDPFLLDF
                     PKMSDMARGVKGTEAVRRVVRALAGRGVNVIKVLATERHGLPQTDPRRRMFMDEELAA
                     VTDEAGKFGLPVAAHAHGEEGAEAAVRAGVRSIEHGTYLNDRTLSLMNERGTYLVPTI
                     YAGVSNSASNPQRSPVVTARSRAMVPPKREMLARALKLGVRIVAGSDAQYIDERRLQD
                     EVAQLVLYGMAPMDAIKAATSVAAECLMISARTGSVRPGLEADLFAVDADPLADVNAL
                     RDVILVVNNGKVAVNRLSF"
     gene            2469..2561
                     /locus_tag="AC159P_21390"
                     /pseudo
     CDS             2469..2561
                     /locus_tag="AC159P_21390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003596034.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2692..2820
                     /locus_tag="AC159P_21395"
                     /pseudo
     CDS             2692..2820
                     /locus_tag="AC159P_21395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611858.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          621 a         1040 c         1021 g          582 t
ORIGIN      
        1 ggctgaggtt gttctcctct acctcggtgt ccggccagat cgcgtccatc agcgcggtct
       61 tgtcgagtac cgaaccgctg tgctccacca ggtaaagcag ggtgtcgaag accttcggcg
      121 tgagcggcac ggggtcaccg ctgctccgca gcaggagacg cctaccgacg tcaattcgaa
      181 agtcaccgaa ctcgtagatg tggtttttcg gcccgttcat gcggctttga ataattctca
      241 gaaactttga agcccatctt aaagactttg agagcctttg gggaagagta tcgccgccgt
      301 cagccgggat gaccggccaa aaattctgat acgggagaca agaaaatgaa actgagaact
      361 gtaaggtcga tgttcggcgc ggcactaacg ctcatccttg ccctcacggc gacccaaccg
      421 tcggcgtccg cgcagggtga gtcgggcaaa ctttcctcgg ggaacgggaa gatcgtcggc
      481 gtgtgggacg tacgggtgac gcgtcgagac tgtcagaccg gcgacgccct gggcgtcaac
      541 ccccccgtca tgaacatgtt caacagcggc ggcacactgc tggagacgtc gagcggcctg
      601 ccgacgaggc gcggccccgg cctgggcacg tggcagtacg tcggcggggg aagctacagc
      661 gccgtcttca ggtttttccg cttcaacgcg gacggcagtt tcgccgggac gcggagggtc
      721 gcacgcacca tcgaactcag ccaggacgcg aacgagttca ccgccacggc caccgacgaa
      781 ctcttcgacg ccagcggcaa tctgttccag gccggctgcg ccaccgaaac ggccacgcgg
      841 ctcgaatagc ccttgaaccg gcgcgaactc agccgccacc tccttctcat ttagtgctga
      901 ggccacatgc gccccgcccc ggtcgccgcc ggcttcgact tctggccctc gaagctcgaa
      961 cggttcggcg gcgacttacg ggggcggatg ataccggcgt caggctggcg gtacatgtgg
     1021 tttggctccg gcgctaagcg tgtttgagtt acgaaggctt tggttcggga ggatatctta
     1081 tgaagttacg attggtgtta acgcttttgc tcttgctgct caacgccgcg ggtcatgttc
     1141 ccgccccgcg ggcacaaaca ccggccacgc ccgcacgcct ggtgatcagg aacgcgacac
     1201 tgatcgacgg cctctcaccc cggctggttc acggcgcgac cgtggtcgtg agtgacggca
     1261 aaatcgaaag tgtttcctcc ggccctgtcg aggttcccgc cggcgcgaca gtgcttgacc
     1321 tgaaagggcg gtggcttttg cccggcctca tcgacgcgca cgtccatttc gttgaccccg
     1381 gcgccgcccg cacggcgctc gcctcgggcg tgacgacggc gagaagcctc ggcgcggagc
     1441 gcttctcgga cgtcggcatc cgcgagctca atcatgaggg cgtcgccgac ctgcccgacg
     1501 tcgtcgcctc cggctaccag gtgacctcgc aggtggggga tcccttcctg ctggacttcc
     1561 cgaagatgag cgacatggcg cggggtgtca aaggcacgga ggccgtgcgc cgggtggtgc
     1621 gcgccctggc ggggcgcggg gtgaacgtga tcaaggttct ggccaccgag cgccacggcc
     1681 tgccgcagac ggatccccgc cggcggatgt tcatggacga ggaactcgcg gccgtgaccg
     1741 acgaggccgg gaagttcggg ctgcccgtgg cggcgcacgc ccacggcgag gagggcgcgg
     1801 aggccgcggt ccgcgcaggg gtccgctcca tcgagcacgg gacttacctc aacgaccgga
     1861 cgctctcgtt gatgaacgaa cgcgggacct acctcgtccc tacaatttac gccggcgtga
     1921 gcaactctgc gagcaacccg cagagaagtc ctgtcgtgac agcccgtagc cgcgcgatgg
     1981 tgccgcccaa gcgtgaaatg ctggcccgcg ccctgaagct gggcgtcagg atcgtggccg
     2041 gcagcgacgc ccagtatatc gacgagcgcc ggctacagga tgaggtggcc caactggtgc
     2101 tctacggcat ggcgccgatg gacgccatca aagcggccac gtccgtcgcc gcggagtgcc
     2161 tgatgattag cgcgcgcacc ggctcggtca gaccggggct ggaggcggac ctcttcgcgg
     2221 tcgacgccga cccgctggcc gacgtcaacg ccttgcgcga tgtgatcctc gtggttaata
     2281 acgggaaggt agcggttaac cgcctgagct tttagggctg ccaccctgcc gccaacagcg
     2341 atgtgggcgt ttgtgctgaa gcatcaaata gtggctagtt acggcagggg cgagatggct
     2401 agcccctccc tgttcccaag ccgctcacat tcaccccgct catgagcccg aatacgctac
     2461 tcgccggagg cgcgcctgcc gccgtgcagg tagctgatcg ctggcacctg ctcaagaacc
     2521 tgcgtgaagc attggagcag atctgtaaac gagtccaacc gagtaaaggc agacagatac
     2581 gacatgcagc acaccacgct acggaacacg aacgcgcgcc gggccactac gccgagcagt
     2641 gccgcatccg gctactgccg catctgctag gccggggctc gggggtaaaa cgcactcaca
     2701 tacgagtgga gtaacggcca actcgagggg caggtgaatc ggttgaagct gatcaagcgg
     2761 cagatgtacg ggcgggcgaa gttcgatctg ctccgggcgc gggtgctcca ttcgacataa
     2821 tgaagttgct tcttatgaga gcttcagggg actgtatcgg cgtcatcaaa atcgcgggag
     2881 agccctgctt gtccgtcgtt ataggacgcg ccgtgcctga ccctaagttt tgcccgaaat
     2941 cacaaagaca tgctccgctt tcaacaacgc ccgtaatgac ttaacgcaat ggcgcgctaa
     3001 cgccgacgcc ccattcaaca cgatccgctt gatcgtgtgc ctgccctcca aatcgacgtg
     3061 cccaccaact tgcaacacgt cgcgcaccac acgctgcaca ccatacttct tcaactgaat
     3121 cttaccccgt ttagtggaga ggcagataag actacccgtt gagcctcacg ccgcctcttt
     3181 caactgcgcg tggtagcacg cttcgtactc cgccggcgcg cagtagtcac tcgctgaatg
     3241 aatcctcacc cggttctaac agac
//
LOCUS       DBNGOH010001097         3528 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_166839, whole genome shotgun sequence.
ACCESSION   DBNGOH010001097 DBNGOH010000000
VERSION     DBNGOH010001097.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3528)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3528)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3528
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_166839"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..517)
                     /locus_tag="AC159P_21400"
     CDS             complement(<1..517)
                     /locus_tag="AC159P_21400"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HIR3698057.1"
                     /translation="MLLQNRYRVTGQIGHGGMGAVFVATDERFRSTVAIKQTFFDNDP
                     SLRKAFEREAHLLNHLRHAALPKVSDHFVEESGQFLVMEFIEGSDLSELLQQRGGSSF
                     PAADALRWADELLDALDYLHTHEPPVVHRDIKPQNLKLTPRGEIVLLDFGLAKGGTGG
                     LDAGVDASLYGY"
     gene            813..2000
                     /locus_tag="AC159P_21405"
     CDS             813..2000
                     /locus_tag="AC159P_21405"
                     /inference="COORDINATES: protein motif:HMM:NF024578.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HIR3698058.1"
                     /translation="MKTKLFLSLVALLIIVAPEVVSAQEAAAPAPPAVVAVPPAEPFE
                     AAPAIAAFFDDGNFLGVVVEEVTRENMGGYGLTGEPRGVAVTRVVKDGPAERAGLREK
                     DVIVRFDGEQVTSVRKLNRLIGESAPGHGARLTIRRGGAEQELTVTLGKRESFMRTFE
                     GLQNIMPLEAEARRRAEESRAQGEAARRGADELRGRADELRRRAEEWQRANPGGVYAF
                     GGGRRIGVSTTSLGKQLADYFGVSNGALVSSVEENSPAAKAGLKAGDIITEAEGEKVD
                     DVGDLSRLLNRKEEGEVTLTVVRDRQRRTVKLTPERRQPQTFNLSPSVYAAPRIAAAP
                     RAPLTVVRPAMARPAAVRPMAGRPALVAPLAPRAVTAPHFRLNTPRTIRMRPDRVVGE
                     RLL"
     gene            complement(2105..>3528)
                     /locus_tag="AC159P_21410"
     CDS             complement(2105..>3528)
                     /locus_tag="AC159P_21410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3698059.1"
                     /translation="SGVLDVLDFVTLVREFYRQSGLDSLLPKYVRSHRAAGDQLRGPA
                     TEMARSVLSYLNTRPVTEIEEKLSAEDPTKSADRKKRDEPRRTITRVKERRFIVTPDL
                     LAPPGAINFRVIGEDYYAVTPVGIDLGPGDLRRVSGLLRRSYVQYMVDPLILRFNREV
                     AAKRAEIKQVADAEAARQNRPPSPDIFLAVARSMVAAADVRMDASARLRALRVETSNR
                     LQAAKDEAARAAVLKESKERQQSVEDEATAGLAEAYERGAVLSFYFAGQLRDVEDSGF
                     DIANFVPNMLSAIDPARELKRPAEYAAAVTRHKEARRQAQQARATEAAAITTAPGAGT
                     SALLKSLNDVEDLLRLKDYEAAEARLAALRNEYREDPRVYFLLGQAASLSAQDAIDGD
                     LQEQRLVAALGHFRQAVLFAASATDPAVVLRAHLASGRILAHLERKDEAAKEFDAVIS
                     KTQPADRLHQEALNEKRKLGGQE"
BASE COUNT          514 a         1140 c         1317 g          557 t
ORIGIN      
        1 tgtagccgta caggctggcg tccacgcccg cgtcgaggcc gccggtgccg cccttggcga
       61 ggccgaagtc cagcagcacg atctcgccgc ggggcgtgag cttgaggttc tgcggcttga
      121 tgtcgcggtg gacgacgggc ggctcgtgcg tgtgcaggta gtcgagcgcg tcgagcagct
      181 cgtcggccca gcgcagcgcg tccgccgccg ggaaggagga gcctccgcgc tgctggagca
      241 gctccgagag gtcggagcct tcgatgaact ccatgacgag gaactggccg gactcttcga
      301 cgaagtggtc gctcaccttc ggcagcgcgg catggcgcag gtggttcaag aggtgcgcct
      361 cgcgctcgaa ggctttgcgc agggaggggt cgttgtcgaa gaaggtctgc ttgatggcga
      421 ccgtgctgcg gaagcgctcg tccgtggcga cgaagacggc gcccatgccg ccgtggccga
      481 tctgtccggt cacgcggtag cggttttgga gaagcatgcc gggctcgatc atggtggctt
      541 tcgggactcc ttcggcagga agcgataggc cggtgtgtcg gtaagcgttg gcgccgtgag
      601 tgcggcgaga ggggcgggcc tcggtcggat tggtcggggt gtgttggccc cggattccag
      661 attaaccgat tggccccgtt ttaatcaata cccgtgtcag gtttcgcatc gctgaagttc
      721 ggcggaaacg cgggccaaaa ttttcggaac cggcgtgcgt gggcgcgcgt gcaagggggt
      781 ggaaaattct caccccggag gtctacgtgt ccatgaaaac gaaattgttc ctgtcgctgg
      841 tcgcgctctt gatcatcgtg gcgccggagg tggtttcggc gcaggaggct gcggcgccag
      901 ccccgcccgc ggtggtggcc gtgccgcccg cggagccgtt cgaggcggcg ccggccatcg
      961 cggccttctt cgacgacggc aacttcctcg gggtggtcgt cgaggaggtg acgcgcgaga
     1021 acatgggcgg ctacgggttg acgggcgagc cgcgcggcgt ggccgtgacg cgcgtcgtga
     1081 aggacggccc ggcggagcgc gcggggctgc gcgaaaagga cgtgatcgtg cgcttcgacg
     1141 gcgagcaggt gacgagcgtg cgcaagctga accgcctgat cggcgagtca gcgcccggac
     1201 acggcgcgcg cctgacgatc cgtcgcggcg gcgcggagca ggagttgacg gtcacgctcg
     1261 gcaagcgcga gagcttcatg cgcacgttcg aggggctgca aaacatcatg ccgctcgaag
     1321 ccgaggcgcg ccgccgcgcc gaggagtcgc gcgcgcaggg cgaagcggcg cggcgcggcg
     1381 ccgacgagct gcgcgggcgt gccgacgagc tgcgccgccg cgccgaagag tggcagcgcg
     1441 ccaaccccgg cggcgtctac gcgttcggtg gggggcgccg catcggcgtc tcgacgacat
     1501 cgctcggcaa acagctcgcg gactacttcg gcgtctcgaa cggcgcgctc gtcagctcgg
     1561 tcgaagagaa cagcccggcg gcgaaggccg ggctgaaggc gggcgacatc atcaccgagg
     1621 cggagggcga gaaggtggat gacgtgggcg acctctcgcg cctcctcaac cgcaaggagg
     1681 aaggcgaggt gacgctcacg gtcgtccgcg accggcagcg ccgcaccgtc aagctgacgc
     1741 cggagcgccg ccagccgcag accttcaacc tcagcccgag cgtctacgcc gcgccccgca
     1801 tcgccgccgc cccgcgcgct ccgcttacag tcgtgcgacc ggcgatggcc cgacccgcgg
     1861 cagtccgacc catggcgggg cgaccggcac tcgtcgcgcc gctcgccccg cgcgcggtga
     1921 cggccccgca cttcagactc aataccccgc gcacgattcg catgaggccc gaccgcgtgg
     1981 tcggcgagcg cctactctaa gcagaagagc gaaaaggtta aaaggggaag aggggaaaag
     2041 ggaggaaggc tcaagccctc tcccttttcc cccttttccc ttttaacctt ttcgcccttc
     2101 tgccttattc ctgcccgccg agcttgcgct tctcgttgag cgcttcctga tggaggcggt
     2161 cggcgggttg ggtcttggag atgacggcgt cgaactcttt ggcggcctcg tccttgcgtt
     2221 cgaggtgcgc gaggatgcga ccgctggcga ggtgtgcgcg caggacgacg gcggggtcgg
     2281 tggcggacgc ggcgaagagg acggcctggc ggaagtggcc gagggcggcg acgaggcgct
     2341 gctcctgcaa gtcgccgtcg atggcgtcct gcgccgagag gctcgccgcc tgaccgagca
     2401 ggaagtagac gcgcgggtct tcgcggtatt cgttcctgag cgccgcgagc cgcgcctctg
     2461 ccgcttcgta gtccttcagc cggagcaggt cttccacgtc gttgagcgat ttgaggaggg
     2521 ccgacgtgcc cgcgccgggc gccgtcgtga tggccgcggc ctcggtcgcc cgcgcctgct
     2581 gcgcctggcg ccgcgcctct ttgtggcgcg tgaccgcggc ggcgtactcg gcggggcgct
     2641 tcagctcgcg cgcggggtcg atggccgaga gcatgttggg gacgaagttg gcgatgtcga
     2701 agccggagtc ctccacgtcg cgcagttgcc cggcgaagta gaaggagagc acggcgccgc
     2761 gctcgtaggc ttcggccagc ccggcggtcg cctcgtcctc gaccgactgc tgccgctcct
     2821 tcgactcctt caggacggcg gcgcgcgccg cctcgtcctt cgcggcctgc aacctgttgc
     2881 tcgtctcgac gcgcagcgcg cgcagcctcg ccgacgcgtc catgcgcaca tccgcggcgg
     2941 ccaccatcga gcgcgcgacc gcgaggaaga tgtcgggcga gggcggtcgg ttctggcgcg
     3001 cggcctcggc gtcggcgacc tgtttgatct cggcgcgctt ggccgccact tcgcggttga
     3061 agcggaggat gagcgggtca accatgtact gcacgtagga gcggcgcagc agccccgaga
     3121 cgcggcgcaa gtcgcccggg ccgaggtcga tcccgacggg cgtgacggcg taatagtctt
     3181 cgccgatgac gcggaagttg atcgcgccgg gcggggccag caagtcgggc gtgacgatga
     3241 accgccgctc cttgacgcgc gtgatggtcc ggcgcggctc gtcgcgcttc ttcctgtccg
     3301 cagacttcgt cgggtcttcc gccgagagct tctcctcgat ctccgtgacg ggccgcgtgt
     3361 tgagataaga gagcacggag cgcgccatct cggtcgcggg gccgcgcaac tggtcgcccg
     3421 ccgcgcggtg gctgcggacg tacttcggca gcagagagtc caggccggac tgccggtaga
     3481 actcgcgcac cagcgtgacg aagtcgagca cgtcgagcac gcccgacg
//
LOCUS       DBNGOH010001098         2733 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2365828, whole genome shotgun sequence.
ACCESSION   DBNGOH010001098 DBNGOH010000000
VERSION     DBNGOH010001098.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2733)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2733)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2733
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2365828"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            227..427
                     /locus_tag="AC159P_21415"
     CDS             227..427
                     /locus_tag="AC159P_21415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3698060.1"
                     /translation="MRKTRLQVCALTVVVAAVGAVSWAARVEADAPAAADAATLKEIA
                     NYKSWTRVTEKPVPGSYLFTGG"
     gene            440..925
                     /locus_tag="AC159P_21420"
     CDS             440..925
                     /locus_tag="AC159P_21420"
                     /inference="COORDINATES: protein motif:HMM:NF028004.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P460 family protein"
                     /protein_id="HIR3698061.1"
                     /translation="MAWRVGDTPVIINSRGTTRILLEGSTVYGSPPPGAKPQKQDVDR
                     AKHVKPAKGEAATYGVVYANDLARAAITSKEPLTYPVGSILVREKLPYTEGDPKPQLL
                     AVMIKRAHGFNPKGGDWEFLIADGAMTKIQERQKKGSCLECHAAQRERDYVFPPPPAP
                     R"
     gene            complement(940..1344)
                     /locus_tag="AC159P_21425"
     CDS             complement(940..1344)
                     /locus_tag="AC159P_21425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3698062.1"
                     /translation="MRGVLLLMGWALFCVSLALPALRTSGFFGFFGGTFRGWFCLAAS
                     VPDIGGFVSGEPRGIYMTLIGLSNVLMLASPALYLWGGRWAAQQRIAFVAAAIVVCLT
                     KFAGGDPSTEYLVGYYVWCASFVAVAAALHFE"
     gene            complement(1372..>2733)
                     /locus_tag="AC159P_21430"
     CDS             complement(1372..>2733)
                     /locus_tag="AC159P_21430"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013714.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HIR3698063.1"
                     /translation="RTRVARLAAPHSLAALQAEIRRARDEGRSVSVAGGRHAMGGQQF
                     ASDAVLLDTTALNRVLGFDPARGEIEVEAGILWPELIAYTLRAQEGRARQVGIVQKQT
                     GADRLSVGGALAANIHGRGLRLRPFVSDVESFVLVSADGEARRCSRVENADLFRLAAG
                     GYGLFGVVASVKLRLAPRRKLVRVVELARLRDLHGLFDRRIADGFLYGDFQFATDPRS
                     EEFLRAGIFSCYRPVADDAPVNESQRELSPADWHELLYLAHTDRRRAFEVYSRHYLAT
                     SGQVYWSDTHQLSVYLDDYHKELDARLGAAERGSEMISELYVPREKLAAYMEAVREDF
                     RRHSTELVYGTVRLIERDEETFLAWAKQDYACVVFNLHVPHTEAGRAKAQVEFRLLID
                     RAIRFGGSYYLTYHRWATRAQVLRCYPQMPEFLRLKRAYDPAELFQSDWYRHHRAMFA
                     GEL"
BASE COUNT          473 a          925 c          962 g          373 t
ORIGIN      
        1 tccccgtcga gcgttacgtc acgcccgagg agttcgcccg ctggcgcgac atcggcatgg
       61 ggatgggctt ccgccacgtc gagtcgagcc cgctcacgcg ctcctcctac cacgcccgcc
      121 agcagaccga cgccgggaca tcctgagcgc ccgcccgccg catccgacct cctgaacggc
      181 tgacaacctc gcccgagcgg caaccggaaa aaacggagga cggatgatga gaaagacgcg
      241 gcttcaagtc tgcgcgctga cggtcgtcgt cgcggcggtc ggcgccgtct cgtgggcggc
      301 gcgcgtcgag gcggacgcgc ccgccgccgc cgacgcggcg acgctcaaag agatcgccaa
      361 ctacaagagc tggactcgcg tgacggagaa gccagtgccc ggcagttacc tcttcaccgg
      421 cggttgaggc ggtggcctcg tggcctggcg tgtcggcgac acgcccgtca tcattaattc
      481 gaggggcacg acgcggattc tgttggaggg gtcaacggtc tacggctcgc cgccgccggg
      541 cgcgaagccg cagaagcagg acgtggatcg ggcgaagcac gtgaaacccg cgaaaggcga
      601 ggccgcgacc tacggggtcg tctacgccaa cgacctcgcg cgcgcggcca tcacgagcaa
      661 ggagccgctc acctacccgg tcggctcgat cctcgtccgc gaaaagctgc cgtacacgga
      721 aggcgacccg aagccgcagc tcctcgccgt catgattaag cgcgcgcacg gcttcaaccc
      781 gaaggggggc gactgggagt tcctcatcgc cgacggcgcg atgacgaaga ttcaggagcg
      841 gcagaagaag ggcagttgtc tcgaatgcca cgccgcgcag cgcgagcgcg actacgtctt
      901 cccgccgccg cccgcgcccc gctgaagccc ctcgcccgtt cactcgaagt gcagggccgc
      961 ggcgaccgcg acgaacgacg cacaccagac gtaatagccg accagatact cggtgctggg
     1021 gtcgccccct gcgaacttcg tcaggcagac gacgatggcc gccgcgacga aggcgatcct
     1081 ttgctgcgcg gcccaccgcc cgccccacag gtagagcgcg ggcgaggcca gcatcagcac
     1141 gttactcagt ccgatgagag tcatgtagat tcccctcggc tcgccgctca cgaagccgcc
     1201 gatgtccggc acgctcgcgg cgaggcagaa ccagcccctg aacgtcccgc cgaagaaccc
     1261 gaagaacccg ctcgtccgca gcgccggcag cgccagcgag acgcagaaga gcgcccaccc
     1321 cattaacagt aagacgccgc gcatggctca accctcctcg ctgcgactcg ttcacaactc
     1381 gcccgcgaac atcgcgcggt ggtggcgata ccagtcgctc tgaaaaagct ccgcggggtc
     1441 gtaggcccgt ttgaggcgga ggaactcggg catctgcggg taacaccgca agacctgtgc
     1501 gcgcgtggcc cagcggtggt aggtgaggta gtagctgccg ccgaaccgga tggcgcggtc
     1561 gatcaagagc cggaactcga cttgcgcctt cgcgcggccc gcctcggtgt gcggcacgtg
     1621 gaggttgaag accacgcagg cataatcctg cttcgcccac gcgaggaagg tctcctcgtc
     1681 gcgctcgatg aggcggacgg tgccgtagac cagctcggtc gagtggcggc ggaagtcttc
     1741 gcgcacagcc tccatgtagg ccgccagctt ttcgcgcggc acgtacagct cggagatcat
     1801 ctcgctgccg cgctcggcgg cgccgagacg cgcgtcgagt tctttgtgat agtcgtcgag
     1861 gtagacgctg agctggtgcg tgtcagacca gtagacttgc cccgaggtgg cgaggtagtg
     1921 gcgcgagtag acctcgaagg cgcggcggcg gtctgtgtgc gcgaggtaga gcagctcgtg
     1981 ccagtcggcg ggcgacagct ctctttgcga ttcgttgacg ggcgcgtcgt cggcgacggg
     2041 gcggtagcag gagaagatgc ccgcgcgcag aaactcttcg gagcgcgggt cggtggcgaa
     2101 ctgaaagtcg ccgtagagga agccgtcggc aatacgacgg tcgaacagtc cgtgcaggtc
     2161 gcgcaggcgg gccaactcca cgacgcgtac gagcttgcgg cgcggggcga gccgcagctt
     2221 gacggaggcg acgaccccga acaggccata gcctccggcg gcgaggcgga acaggtctgc
     2281 gttctcgacg cggctgcaac gccgcgcctc gccgtccgcc gagacgagga cgaacgactc
     2341 cacgtcggag acgaagggcc ggaggcgcag gccgcgcccg tggatgttgg cggcgagcgc
     2401 gccgccgacc gagaggcggt cggcgccggt ctgcttctgc acgatgccga cctgccgcgc
     2461 gcggccttcc tgcgcgcgca aggtgtaggc gatcagctcg ggccagagga tgccggcctc
     2521 gacttcgatc tcgccgcgcg cggggtcgaa gccgaggacg cggttgagcg ccgtcgtgtc
     2581 gagcagcacg gcgtcggacg cgaactgctg cccgcccatc gcgtgcctgc cgcccgcgac
     2641 ggagacggag cggccttcgt cgcgggcgcg cctgatctcg gcttgcagcg ccgcgagcga
     2701 gtggggcgcg gcgaggcgcg cgacgcgcgt gcg
//
LOCUS       DBNGOH010001099         2006 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3832261, whole genome shotgun sequence.
ACCESSION   DBNGOH010001099 DBNGOH010000000
VERSION     DBNGOH010001099.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2006)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2006)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2006
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3832261"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..596
                     /locus_tag="AC159P_21435"
     CDS             <1..596
                     /locus_tag="AC159P_21435"
                     /inference="COORDINATES: protein motif:HMM:NF027875.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycogen-binding domain-containing protein"
                     /protein_id="HIR3698064.1"
                     /translation="RGKVAGTPVRFNRDDEGRVTSVTFVHEGESYRAPRVELPPPSLK
                     GNTTFRLKGHADATIVAVAGSFNNWNQSQLLFAREGDEWVCRVDLDPGVYTYKLIVDG
                     NWLLDPANPSTQEDEAGNVNNVLEVKPEKQKAAGSRQRAVKTLSAALSGCRRQRPPEL
                     PDCLLPAAYCLPLFCVKSIPAQLLNPDYKPGGFINPS"
     gene            593..>2006
                     /gene="ettA"
                     /locus_tag="AC159P_21440"
     CDS             593..>2006
                     /gene="ettA"
                     /locus_tag="AC159P_21440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HIR3698065.1"
                     /translation="MSTQPNKIIYSMVGVSRHYDKRAVLKDIYLSYFYGAKIGVIGLN
                     GSGKSSLLRIIAGADKGFTGETVLSPGYTVGYLEQEPRLDESKTVREVVEEAVAETVS
                     LLKEFDEINAKFGEEMSDDEMTALLERQGAVQEKLDASDAWDLDSRLEMAMDALRCPP
                     GETPVKVLSGGEKRRVALCRLLLMKPDILLLDEPTNHLDAESVAWLEQHLQRYEGTVI
                     AVTHDRYFLDNVAGWILELDRGEGIPWKGNYSSWLEQKQERLRREEKAEGERQKSLQR
                     ELEWIRMAPKARQAKGKARINAYEAMLNEQSEARARELEIYIPPGPRLGQIVIEADSV
                     SKAYGANLLVEGMTFRLPPGGIVGVIGPNGAGKTTLFRMITGQEQPDAGTIRVGETVK
                     LAYVDQSRDVLDANKSIWEEISEGRDTVQLGPREVNSRAYVGRFNFSGSDQQKKVGLL
                     SGGERNRLHLAKLLRAGSNLL"
BASE COUNT          394 a          644 c          658 g          310 t
ORIGIN      
        1 agcgcggcaa ggtcgcgggc acacccgtcc gtttcaaccg cgacgacgag gggcgcgtca
       61 cgagcgtcac cttcgtccac gagggcgaga gttacagggc gccccgcgtc gagctgccgc
      121 cgccgagcct caagggcaac accaccttcc gcctcaaagg ccacgccgac gcgaccatcg
      181 tcgccgtcgc cggctccttc aacaactgga accagtcgca gctcctcttc gcgcgcgaag
      241 gcgacgagtg ggtctgccgc gtcgatctcg accccggcgt ctatacctac aagctcatcg
      301 tggacggcaa ctggctcctc gaccctgcta acccctcgac gcaggaggac gaggccggca
      361 acgtcaacaa cgtcctcgaa gtgaagccgg aaaagcagaa ggccgcaggc agtaggcagc
      421 gggcagttaa aaccttgtct gccgcgctgt cgggttgcag gcgtcagcgt ccgccggagc
      481 ttcctgactg cctactgcct gctgcctact gcctaccgct tttctgtgtt aaatctatac
      541 ccgcacaact actcaatccc gattacaaac ccggaggttt cataaatccg tcatgagcac
      601 acaaccgaac aaaatcatct actcgatggt cggcgtgagc cgccactacg acaagcgcgc
      661 ggtcttgaag gacatctacc tctcttactt ctacggcgcg aagatcggcg tcatcggctt
      721 gaacggctcg ggcaagtcca gcttgctgcg catcatcgcg ggtgcggaca agggcttcac
      781 gggcgagacc gttctgtcgc cgggctacac ggtgggctac ctcgaacagg agccgcggct
      841 cgacgagtcg aagaccgtgc gcgaggtggt cgaagaggcg gtcgcggaga ccgtgagcct
      901 cttgaaagag ttcgacgaga tcaacgcgaa gttcggcgag gagatgtcgg acgacgagat
      961 gacggcgctc ctcgaacggc agggcgcggt gcaggagaag ctcgacgcgt cggacgcgtg
     1021 ggatttggac tcgcggctgg agatggcgat ggacgcgctc aggtgcccgc cgggagagac
     1081 gcccgtcaag gtcttgtcgg gcggcgagaa gcggcgcgtc gcgctgtgcc gcctgctctt
     1141 gatgaagccg gacatcctgc tgctggacga gccgacgaac cacctcgacg cggagtcggt
     1201 ggcgtggctc gaacagcact tgcagcgcta cgagggcacg gtcatcgccg tcacgcacga
     1261 ccgctatttc ctcgacaacg tcgcgggctg gattctcgaa ctcgaccgcg gcgagggcat
     1321 cccgtggaag gggaactact cttcgtggct cgaacagaag caggagcgtt tgcggcgcga
     1381 ggagaaggcc gagggcgagc ggcagaagtc cttgcagcgc gagctggaat ggattcgcat
     1441 ggcgccgaag gcgcggcagg ccaaaggcaa ggcgcgcatc aacgcctacg aggcgatgct
     1501 caacgagcag agcgaggccc gcgcgcgcga gcttgaaatc tacatcccgc ccggcccccg
     1561 cctcggccag atcgtgatcg aggccgactc ggtgtcgaag gcttacggcg cgaacctgct
     1621 cgtcgaaggc atgacgttcc gtctgccgcc cggcggcatc gtcggcgtca tcggcccgaa
     1681 cggcgcgggt aaaacgacgc tcttccggat gatcacgggc caggagcagc ccgacgcggg
     1741 gacgattcgc gtgggcgaga ccgtcaagct cgcctacgtc gatcagagcc gcgacgtgct
     1801 cgacgcgaac aagtcgatct gggaggaaat ttccgaaggg cgcgacaccg tgcagctcgg
     1861 gccgcgcgag gtcaactcgc gcgcctacgt cgggcgcttc aacttctccg gctccgacca
     1921 gcagaagaag gtcggactcc tctcgggcgg cgagcgcaac cggctgcacc tggccaagct
     1981 cctcagggcc ggatcgaatc tgctgc
//
LOCUS       DBNGOH010001100         2268 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4749613, whole genome shotgun sequence.
ACCESSION   DBNGOH010001100 DBNGOH010000000
VERSION     DBNGOH010001100.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2268)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2268)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2268
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4749613"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(196..1197)
                     /locus_tag="AC159P_21445"
     CDS             complement(196..1197)
                     /locus_tag="AC159P_21445"
                     /inference="COORDINATES: protein motif:HMM:NF019604.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HIR3698066.1"
                     /translation="MTTTRDRLDLLIVGAGPAGLSAADAAAREGLTYLVVEKGLIADT
                     VYHYPIGLTVFSTVNELELREGALRPCREKPTREELLSHYVRFALEEGLRINTEEAVT
                     RITRLDPEGFLVRTSRAEYEAARLLVCTGAMARPRRLDVPGEDLAKVHHRFVEPFPYV
                     RKDVLVVGGGNSAAEAALFLSEGGARPTFAIWRTDWENRDPKLGCIKHWVRGPLEREI
                     AEGRLRLFPFTEVTGIDERHVRLKDEHGAPHTLPNDAVFILIGADADHTLLRQVGVNI
                     VPAGLAEVPSYDPDTYETNVPGLHVAGHFTNSRHIKEAIAVPRLIVPLIAQSLRATV"
     gene            complement(1266..>2268)
                     /locus_tag="AC159P_21450"
     CDS             complement(1266..>2268)
                     /locus_tag="AC159P_21450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020407322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3698067.1"
                     /translation="LFHVLNFALNLTSGSRLDWQDRKSESFTASPLHCGSFQPADRRF
                     SREPLGYRDARRYGGDNGISLGTSVAISGAFVSPNMGYMMTSPVVRFLMTLFNVRFGW
                     WLGNPRHTTYRRGSPRLSVKPIAMEALGMTDDRASYVYLSDGGHFENLGLYEMVMRRC
                     HFIFVSDASTDNEYSFGSLGMAVRQIRVDLGVPIEFTDFTVIPPAEGKDGRSCAIGRI
                     RYSCVDKQAPDGVLVYLKPTLTGSEPRDVLNYARDNPSFPQEVIVDQWFSEAQFESYR
                     ALGSYIVNDLAGAGDSRSDQRPEDFLHNVEKYIGRNPSPGLRELVRESLGEIIRKDA"
BASE COUNT          348 a          665 c          881 g          374 t
ORIGIN      
        1 tgaaaccccc acccgcggcc cgcgcacgag cctgaaccgg gggccgcacg ccgcttaacc
       61 aggcagggcg cgaagaggaa cggcaagtcc tcttcgcgcc ctgcctggtt aagcggttaa
      121 tcctgccggc ttacgtctgc ggcttcgcgg ctttgcgcgt gcgcgtccca ctctggactt
      181 atttgccgga aaaattcaaa ccgtcgcgcg cagggattgg gcgatgaggg ggacgatgag
      241 gcgcgggacg gcgatggctt ctttgatgtg gcgcgagttg gtgaagtggc cggcgacgtg
      301 gaggccgggg acgttggtct cgtaggtgtc ggggtcgtaa gaggggactt cggcgaggcc
      361 ggcggggacg atgttgacgc cgacttggcg gagcagggtg tggtcggcgt cggcgccgat
      421 gaggatgaag acggcgtcgt tggggagcgt gtgcggggcg ccgtgttcgt ccttgaggcg
      481 gacgtggcgc tcgtcgatcc ctgtgacttc ggtgaagggg aagaggcgga ggcggccttc
      541 ggcgatctcg cgttcgagcg ggccgcgcac ccagtgtttg atgcagccca gcttggggtc
      601 gcggttctcc cagtccgtgc gccagatggc gaaggtgggg cgggcgccgc cttcggagag
      661 gaagagcgcg gcctcggcgg cggagttgcc gccgccgacg acgagcacgt ccttgcggac
      721 gtaagggaaa ggctcgacga agcggtggtg gactttcgcg aggtcttcgc cgggcacgtc
      781 gaggcggcgc gggcgcgcca tcgcgccggt gcagaccagc aggcgcgcgg cctcgtactc
      841 ggcgcgcgag gtgcggacga gaaagccctc ggggtcgagg cgcgtgatgc gcgtgaccgc
      901 ctcttcagtg ttgatgcgga ggccctcttc gagggcgaag cggacgtagt gcgagagcag
      961 ctcctcgcgt gtcggttttt cgcggcaggg gcgcagagcg ccttcgcgca gctcaagctc
     1021 gttcaccgtg gagaagaccg tcaggccgat ggggtagtgg tagaccgtgt cggcaatgag
     1081 tcccttctcg acgacgaggt aggtcagccc ctcgcgcgcg gcggcgtcgg cggcggagag
     1141 gccggcgggg ccggcgccga cgatcaggag gtcgagtctg tcgcgcgttg tcgtcatggg
     1201 cttgaccgac gacatcgtcg cacgccgcgt cgcggaatat caaacgtgcg ccgcctttcg
     1261 cgccctcacg cgtctttgcg aatgatctcg cccaaggact cgcgcaccag ctcgcggagt
     1321 ccgggcgagg ggttgcgccc gatgtacttc tcgacgttgt gcaggaagtc ttcggggcgc
     1381 tggtcgctgc gcgagtcgcc ggcgcccgcg aggtcgttga cgatgtagga gccgagggcg
     1441 cggtagctct cgaactgcgc ctcgctgaac cactgatcga cgatgacctc ctgcgggaac
     1501 gaggggttgt cgcgcgcgta gttgagcacg tcgcgcggct cgctgcccgt cagcgtcggc
     1561 ttcaggtaga ccagcacgcc gtcgggcgcc tgcttatcga cacacgagta gcggatgcga
     1621 ccgatggcgc acgacctgcc gtccttgccc tcggcgggcg ggatgacggt gaagtcggtg
     1681 aactcgatgg ggacgccgag gtctacgcgg atctggcgca cggccatgcc gagcgagccg
     1741 aacgagtatt cgttgtccgt gctcgcgtcg ctgacgaaga tgaagtggca gcggcgcatg
     1801 accatctcgt agaggccgag gttctcgaag tggcccccgt cggagaggta gacgtaggac
     1861 gcccggtcgt cggtcatgcc gagcgcctcc atcgcgatcg gcttgacgga gaggcgcggg
     1921 ctgccgcggc ggtaggtggt gtggcgcggg ttgccgagcc accagccgaa gcgcacgttg
     1981 aagagcgtca tcaggaagcg cacgaccggc gaggtcatca tgtagcccat gttcgggctg
     2041 acgaacgcgc cggagatggc gaccgaggtg ccgagcgaga tgccgttatc gccgccgtac
     2101 cggcgcgcgt cgcggtagcc gagcggctcg cgcgagaagc ggcggtcggc gggctggaag
     2161 ctgccgcagt ggagcggcga ggccgtgaac gactcggact tgcgatcctg ccagtcgagg
     2221 cggctcccgc tcgtcaggtt gagcgcgaag ttgagcacgt ggaagagc
//
LOCUS       DBNGOH010001101         5590 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_351432, whole genome shotgun sequence.
ACCESSION   DBNGOH010001101 DBNGOH010000000
VERSION     DBNGOH010001101.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5590)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5590)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5590
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_351432"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1201)
                     /locus_tag="AC159P_21455"
     CDS             complement(<1..1201)
                     /locus_tag="AC159P_21455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014098929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HIR3698068.1"
                     /translation="MSRTSEQTTGTLAASADGAPPRGVTAALNLPPHLDEHTPPEVRA
                     AVELARRYRLPYVDLLPPEGPSPIDYEILGELPIDLMVRYQFVPLRREGKRLHVAMAD
                     PTDLEKLDEIASALHARLVPHVATAGAIDVVLRKGDATQRVLQEATSGFRISLVKETE
                     QGEEVLDLDRLATDSEMSPIIKLVDTIIYQAMESRASDIHLESDDSALRVKYRIDGAL
                     YEKVDPIDIAYHSTLISRIKVMSELDIAERRVPQDGRFRVRYKGRNVDFRVSVMPTAF
                     GEDAVIRILDKEQINEQFRNLSLDIIGFSDENIQGFRRYIAEPYGMVLVTGPTGSGKT
                     TTLYAALNEIRNEEDKIITIEDPIEYQLHGITQIPVNEKKGLTFARGLRSILRHDPDK
                     IMVGEIRD"
     gene            complement(1238..2473)
                     /locus_tag="AC159P_21460"
     CDS             complement(1238..2473)
                     /locus_tag="AC159P_21460"
                     /inference="COORDINATES: protein motif:HMM:NF012694.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HIR3698069.1"
                     /translation="MAEFIRRLGTPAGEVVTRTVEAAGAGEARVRLEAEGFRVFSVLP
                     PKAAKGIASFTGGGKSGTQARVKPGDFLLFNQQLAAIIRAGIPILQAIAMLRRRAASA
                     RLRMVLADVEDKIRGGMALSQAFAAQGSIFPRIYTASILAGERSGALDDVLSRYVTYM
                     RRGVEIRRKIRGALAYPVFLLLASLGMVVFLTGYVVPRMSSLFEGFNTELPAVTALVI
                     GGSNFFAANFYWLIPTVIGAAIALGIWWATPSGKLAIDRLLLKLPIASALIKQLSIAQ
                     VTRSLATLLAGGITLVESWEIAAEAITNRELRARSSATLPMIQEGRSFTESLENAGWI
                     PPLALDMIGVGERSGSLKEMLEEVAEFYDKESEVRLEQLTTVLEPAILLVMGGIVVTI
                     LLAIYLPIIQSISNINVRR"
     gene            3023..4252
                     /locus_tag="AC159P_21465"
     CDS             3023..4252
                     /locus_tag="AC159P_21465"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HIR3698070.1"
                     /translation="MRAQRLRGFLRAAFVPCLVVTSAFVIAAPAHVAAQPQAPTEQKT
                     EQQNAQQPPAAPKAADTAAPKPTDTTPSPAAAGASSASTTNTAPPNNNTTTAPATTGA
                     ANAGEANNATAPGAAAPLGPGVPVDDSKTIISHTDLVTVTVTVTDMYGRFVTGLGKNA
                     FSVFEEKAAQDILFFSDEDAPVSLGVVFDVSGSMSGDKIMKAREALSRFIDTSHKNDE
                     YFLIGFNHRPQLLLNRTRDSDALLDKLTFVQTKGQTALYDATYLGVERVTRGSHKKRA
                     ILLISDGQDNSSRYTYTEVKRMLKESDVIIYAVGIIGGGGSGELDMSGRAILEELAGV
                     SGGKAFFPSTSAEMNDTFERIALELRTQYSISYRPSNFTNDGKWRKIKVKVQPPRGFP
                     RLFVRGREGYFAVTNPK"
     gene            4680..>5590
                     /locus_tag="AC159P_21470"
     CDS             4680..>5590
                     /locus_tag="AC159P_21470"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HIR3698071.1"
                     /translation="MRNREVRVAPAGAVPRALVASLFAAALSVGPVAPSRAQLNDGGR
                     PRRVVRPASQTAAAPPQQRTTTTTTTQQPPAQKSPTPAQTPTPTPAASPQTTPAPGGP
                     PKLQVQTPVKIEAAPGEKTPTEQEVNEDEVVRIESNEIKLHVRVIDRNNRPVGDVRKE
                     EFRVFENGQPQTVTSFTKEEVPVSYGLVVDNSGSLRQQITQVIDAAKTIVESNKPGDE
                     TFVVRFVSSDDIEIKQDFTADKQSVQDALEDMFVEGGQTAVVDAVYLSSEHAAEHKKG
                     NELDDKRRRALILVTDGEDRNSHYKVE"
BASE COUNT         1004 a         1906 c         1805 g          875 t
ORIGIN      
        1 tgtcgcggat ctcgcccacc atgatcttgt ccgggtcgtg gcgcaggatc gagcgcaggc
       61 cgcgcgcgaa cgtcaagccc ttcttctcgt tcacgggaat ctgcgtgatg ccgtggagct
      121 ggtactcgat cgggtcttcg atggtgatga tcttgtcttc ttcgttgcgg atctcgttga
      181 gggcggcgta cagagtcgtc gtcttgccgg agccggtggg gccggtgacg agcaccatcc
      241 cgtacggctc ggcgatgtag cggcggaagc cctggatgtt ctcgtcggaa aaaccgatga
      301 tgtcgaggga caggttgcgg aactgctcgt tgatctgctc cttgtcgagg atgcggatga
      361 ccgcgtcctc gccgaacgcc gtgggcatga cggagacgcg gaagtccacg ttccgccctt
      421 tataacgcac gcggaagcga ccgtcctgcg ggacgcgccg ctcggcgatg tcgagttccg
      481 acatgacctt gatgcgcgag atgagggtcg agtgataggc gatgtcgatg ggatcgacct
      541 tctcgtagag cgcgccgtcg atgcggtact tgacgcgcaa ggctgaatca tcactttcga
      601 gatggatgtc ggaggcgcgc gactccatcg cctgatagat gatcgtgtcc accagcttga
      661 tgatgggcga catctcggag tcggtcgcca atctatcaag gtccaaaacc tcttcgccct
      721 gctcggtctc cttgacgagc gagatgcgga agccggaggt cgcttcctga aggacgcgct
      781 gcgtggcgtc gcccttgcgc agcaccacgt cgatggcccc ggcggtggcg acgtgcggga
      841 cgaggcgggc gtggagcgcg ctggcgatct cgtccagctt ttcgaggtcg gtcgggtcgg
      901 ccatcgccac gtgcaggcgc ttgccttcgc gccggagcgg cacgaactgg tagcgcacca
      961 tgaggtcgat gggcagctcg ccgaggattt cgtagtcgat cggcgagggg ccttcgggcg
     1021 ggagcagatc gacgtaaggg agccggtagc gccgcgccag ctcgaccgcc gcgcgcacct
     1081 cgggcggcgt gtgctcgtcg aggtgaggcg gcaggttcag cgcggcggtg acgccgcgcg
     1141 ggggcgcgcc gtccgccgac gcggcgagag tccccgtggt ctgttctgag gttcggctca
     1201 tgagagtttt cgatgcttga cggcggcggg ggattgccta gcgtctgacg tttatgttcg
     1261 agatggactg gatgatcggc agatagatcg ccagcaggat cgtgaccacg atcccgccca
     1321 tcaccagcag aatcgcaggt tcgagcaccg tcgtcaactg ctccaaacgc acctccgact
     1381 ccttgtcgta gaactcggcg acctcctcca gcatctcctt caaactgccc gagcgctcgc
     1441 ccacaccgat catgtcgagc gccaaaggcg gtatccaccc ggcgttctca aggctctcgg
     1501 tgaacgagcg cccttcctga atcatcggca gcgtggcgga gctgcgggcg cgcagttccc
     1561 tgttcgtgat ggcctcggcg gcgatctccc acgactcgac gagcgtgatg ccgcccgcca
     1621 gcagcgtcgc cagagagcgc gtcacctgcg cgatcgagag ctgcttgatg agcgcactgg
     1681 cgatgggcag tttcagcagg aggcggtcga tggcgagctt gccgctcggc gtcgcccacc
     1741 agatgccgag ggcgatggcg gcgccgatga cggtcgggat gagccagtag aagttggcgg
     1801 cgaagaagtt cgagccgccg atgacgagcg ccgtcaccgc cggcagctcc gtgttgaagc
     1861 cctcgaagag gctcgacatg cgcggcacga cgtagcccgt caggaagacg accatgccca
     1921 gagaggccag cagcaggaag accgggtagg cgagcgcgcc gcgaatcttg cggcggattt
     1981 cgacgccgcg gcgcatgtag gtgacgtaac gcgagagcac gtcgtcgagc gcgcccgagc
     2041 gctcacccgc caggatcgag gccgtgtaga tgcgcgggaa gatcgacccc tgcgccgcga
     2101 acgcctgcga cagcgccatg ccgccgcgga ttttatcctc cacgtcggcg agcaccatcc
     2161 gaaggcgcgc gctggccgcg cggcgcctga gcatggcgat ggcctgaagg atcgggatgc
     2221 ccgcgcggat gatggcggcg agctgctggt tgaagaggag gaagtctccg ggcttgacgc
     2281 gcgcctgcgt gccggacttg ccgccgcccg taaaagacgc gatgcccttc gccgccttcg
     2341 gcggcagcac ggagaagacg cggaacccct cggcttcgag ccgcacgcgc gcctcgcccg
     2401 cgcccgccgc ctcgaccgtg cgcgtcacca cctcgcccgc gggcgtcccc aaacgcctga
     2461 tgaactcggc cattacgctt ctgtttgaat ctactggttt ggcgccttgt cgcccgcgag
     2521 cgcgcggcgc gtgagtttat cgaagacctc gaccccccga ttgtatcacg ccgttcggcg
     2581 gcgccgggag cggcgccccc ttccgatgcg ggtgacgctt ccgacttcgg acgcgacttc
     2641 gagacgccct tcggatgccg tgcccccgcg gcccggcccc caccgtcgca cctcgggcgg
     2701 ggccgggccg cgggggcacg gcgtcgccct ccgtacactt tcacgcgcga ggcggcgcgg
     2761 agttgcgtgg agatagtctt gaaacaaaat cggaacagag atgacggttg caaggccggc
     2821 ggcgcgtgtc atagaatgcg cttgaagagg aaacacaacg gagtctcccc gcctccgctt
     2881 ctctgtcccg cggacaacct aaagggcgcg acccgccgtc aaagcgggca cacgaaggaa
     2941 acctgggaga gcctttccta agcttccaca ctttctacgt ctttttgttg tcccccagcc
     3001 tgtcagtcgt cgggagggtc atatgagagc acagcgcctc cgcgggttct tgagagccgc
     3061 gttcgtcccc tgccttgtcg tcacgtcagc gttcgtcatc gccgcacccg cgcacgtcgc
     3121 agcacagccg caggcgccca cggaacagaa gaccgagcag cagaacgccc agcagccgcc
     3181 cgccgcgccg aaggccgccg acaccgccgc gccgaaaccg accgacacga ccccttcgcc
     3241 cgccgccgca ggcgcctcgt cggcttcgac aaccaacacc gcccccccca acaacaacac
     3301 gaccaccgcg cccgccacga cgggcgccgc caacgcgggc gaggcgaaca acgcgaccgc
     3361 gccgggcgcc gccgccccgc tggggccggg cgtcccggtc gatgacagca agacgatcat
     3421 ctcgcacacc gacctcgtca ccgtgacggt gacggtgacg gacatgtacg ggcgcttcgt
     3481 gacgggcctc ggcaaaaacg ccttctcggt cttcgaggag aaggcggcgc aggacatcct
     3541 cttcttcagc gacgaggacg cgcccgtcag cctcggcgtc gtcttcgacg tgtccggctc
     3601 gatgagcggc gacaagatca tgaaggcgcg cgaggcgctc tcgcgcttca tcgacacgag
     3661 ccacaagaac gacgagtatt tcctcatcgg cttcaaccac cgcccgcagc tccttctcaa
     3721 ccgcacgcgc gacagcgacg cgctcttgga caagctgacc ttcgtccaga ccaagggcca
     3781 gaccgcgctc tacgacgcca cctatctcgg cgtcgagcgc gtgacgcgcg gctcgcacaa
     3841 aaagcgcgcc atcctcctca tctccgacgg gcaggacaac agctcgcgct acacctacac
     3901 cgaggtcaag cggatgctga aggagtcgga cgtgatcatc tacgccgtcg gcatcatcgg
     3961 cggcggcggc tcgggcgaac tcgacatgtc gggccgcgcc atcctcgaag agctggccgg
     4021 ggtctccggc ggcaaagcct tcttcccctc gaccagcgcc gagatgaacg acacgttcga
     4081 acgcatcgcg ctcgaactgc gcacgcagta ttccatcagc taccgcccat cgaacttcac
     4141 caacgacggc aagtggcgca agatcaaagt caaagtgcag cccccccgcg gcttcccccg
     4201 cctcttcgtc cgcgggcgcg aaggctactt cgccgtcaca aatccaaagt agaggtcaga
     4261 ggtcagaggt cggaggtcag ttaaaaacaa gcaaggagag gccggaggcc agcgacgacg
     4321 aagctgcttt tggcctctct tgtgcttcct gtctttcact ggcctccggc ctctgctttc
     4381 tggcaacggc gcagtcccac gcggcatctc taaacaaggt gcgcgcagcc gtttcaggcg
     4441 cgcgtttttc aaggcattac gggtataatg acaagcgccc cggccacgcg gagccgccgc
     4501 cgccgaaagc gtcggcccgg agaccgtaag cgccccgcgc cctcccgacc ccgtacacgg
     4561 cgggcggaca cggggcgctg cttttgagag gctggagact tgaggataga gactcgtcaa
     4621 aacatttctg ctagtctcta accccaagtc tctagcctct gtccgttgag gttgtgccga
     4681 tgagaaatcg agaagtccga gtcgcccccg cgggggcggt gccgcgcgcg ctcgtcgcgt
     4741 cgctgttcgc cgccgcgctg tcggtcggcc ccgtcgcccc ctcgcgggcg cagttgaacg
     4801 acggcgggcg cccgcgccgc gtcgtccggc ccgcgtcgca gaccgccgcc gcgccgccgc
     4861 agcagcggac gaccacgacc acgacgacgc agcagccgcc cgcgcagaag tcgcccaccc
     4921 ctgcgcagac cccgacgccg acacccgccg cctcgccgca gacgacgccc gcgccgggcg
     4981 gcccgcccaa gctacaggtg cagacgcccg tcaagatcga ggcggcgccg ggtgagaaga
     5041 cgccgaccga gcaggaggtc aacgaggacg aggtcgtccg catcgagagc aacgagatca
     5101 agctccacgt gcgcgtcatc gaccgcaaca accgccccgt cggcgacgtg cgcaaggaag
     5161 agttccgcgt cttcgagaac ggccagccgc agaccgtcac ctccttcacg aaggaggagg
     5221 tgcccgtctc ttacggcctc gtcgtggaca actccggctc cctgcgccag cagatcacgc
     5281 aggtcatcga cgccgccaag accatcgtcg agagcaacaa gcccggcgac gagaccttcg
     5341 tcgtgcgctt cgtgtcgagc gacgacatcg agatcaagca ggacttcacc gccgacaagc
     5401 agtccgtcca ggacgccctc gaggacatgt tcgtcgaggg cgggcagacc gccgtcgtcg
     5461 atgccgtcta tctttcgagc gagcacgccg ccgagcacaa gaaggggaac gagctggacg
     5521 acaagcgccg ccgcgcgctg atcctcgtca ccgacggcga agaccgcaac agccattaca
     5581 aggtcgagca
//
LOCUS       DBNGOH010001102         6888 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1450692, whole genome shotgun sequence.
ACCESSION   DBNGOH010001102 DBNGOH010000000
VERSION     DBNGOH010001102.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6888)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6888)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6888
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1450692"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(505..1020)
                     /locus_tag="AC159P_21475"
     CDS             complement(505..1020)
                     /locus_tag="AC159P_21475"
                     /inference="COORDINATES: protein motif:HMM:NF024816.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3698072.1"
                     /translation="MPLFTESLTAARRAEDKYLEAVCLHNLGDAAMQTGRLERAEECL
                     ADSLALFELLGHRPLAARTALLQGEVAVLRGDTASAHARQRGALAELKEIGDTQGIAT
                     ALEAIACAQSTRQPRLALVLYGAAAGLRVKNQAPSGAGPPTGDRTMHEARANGGGRRG
                     GRGWPQPKEPR"
     gene            complement(971..1645)
                     /locus_tag="AC159P_21480"
     CDS             complement(971..1645)
                     /locus_tag="AC159P_21480"
                     /inference="COORDINATES: protein motif:HMM:NF044287.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR3698073.1"
                     /translation="MSLIDLAAQDDLRLADEIDDIGRAGVAPERLTRFVGRTAEVARI
                     LELLRRTRLVTLTGPPGIGKTRLATEVAAGVSQNYTHGVCAVDFVPISDEVLVPKALM
                     TALGLSEQAGRAVEETLREHLKDKHLLLVFDNCEHVIEACAGLCERLLRASRLLQVIA
                     TSREALNIPGETVLEVTPLSFPPGERSADAEAAARYEAAARPGRGLASDAALHGELDG
                     GAACGR"
     gene            1692..2123
                     /locus_tag="AC159P_21485"
     CDS             1692..2123
                     /locus_tag="AC159P_21485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3698074.1"
                     /translation="MSEAVRGQVGAGYTFRQFGLLKPRRMRNGKVNLNTSPAGWAKYA
                     VDEEVVLFLYRQAAWTGLRTTVGLGQGQLEVRAGNIASQFDNMGLFENVTVEHNLLND
                     SDKRLLGTKKGAVNGDSFMSFVRRAINDKWIEKGKMRHAKK"
     gene            2187..4364
                     /locus_tag="AC159P_21490"
     CDS             2187..4364
                     /locus_tag="AC159P_21490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3698075.1"
                     /translation="MFDEANRIPYRYDVSSPVPVYTDLGPFEIVPSPHVPITNERADE
                     IVAFAFKQWTDVPTSAFQAQVIGDFASIGLRDVKDAATAALVIGPENGGGIHVIYDAD
                     GSILRDFFGTSPSVLGIASPEWADETTGTLTESWAVINAQQRWVGDQELLNYAGVFTH
                     EFGHAINLAHSQKTGAVAFFGDARGPAGCTTLPYPTTMTINDIETMYPFINPRPTTGS
                     GLAQSTVDRTDDMAAVSDLYPAPGYPQTHGSIKGRVLAADGKEGLTGVNVIARNVDHP
                     FADAVLAMSGDYTRLAVGPDGSFTLTGLTPGARYVLYTDAIVSGGFPTPQPFFIPGPE
                     EFFNAEGESGDGVTDNRCEAAPVTAVAGLTTEADITLNAVKGAPKFIPIGPGTIPNSI
                     TGDGRVVGGSIQQGPVFRWTEEGGVEVLGTARTVNGTMSRDGSVFGGETEQANGLRVA
                     SLLRAGAPWETLPLPAGITPCGTDATTSYGVAEIGKATSGAAWVSGCRVRPFLWTPTG
                     GTQMLPTPANVSNSRPNTISADGSTVVGWWQSTGNSSRRGARWVNGQFEEFSTPELSV
                     GEAQNTNPDGSVIVGAGAGPRQEPWRWTPDGGVQFLGRLAPFGFAGASAISDDGQIIG
                     GVGGGSSISPATRISFLWMPKLGTVNFEQFLRAQGTYFGDSILNAPLAMSSDGQVIVR
                     LRPRHRRLGGADGQGDRLPRAAGQPRERPVAPRCVPRQPRRAP"
     gene            4608..5042
                     /locus_tag="AC159P_21495"
                     /pseudo
     CDS             4608..5042
                     /locus_tag="AC159P_21495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013039543.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
     gene            5208..>6888
                     /locus_tag="AC159P_21500"
     CDS             5208..>6888
                     /locus_tag="AC159P_21500"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024583.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3698076.1"
                     /translation="MRELVHEDDAARALGVVQRSVEERNDYDIEYRVRRPDGSQRWVM
                     SRGRAVYDEGGGLVGMLGVVQDITQRKETEERLREQSETLRTVHDIGQIVSAELDLQK
                     VVQAVTDAATELTGARFGSFFYNVLDERGASYMLYTLSGVPAEAFSHFPMPRATDLFG
                     PTFRGEGVLRIDDVHRDPRYGKNSPYYGMPEGHLPVVSYLAVPVASRSGEVVGGLFFG
                     HPEPGVFTARHERVVAGLAAQAAVAMDNARLYEAAKRARAEAEAAARENELLYRDARE
                     ANRLKDEFLATVSHELRTPLTAILGWSHMLRLAQLDEAAAVRAFETIERNARAQAQLI
                     DDLLDVSRAITGKLRIDVRAVDPNSFIEAAIEAVRPAADAKNVRVQKIIDTGVVSVSG
                     DPVRLQQVVWNLLSNAIKFTPKGGRVQVRLQRINSHIEIAVSDTGAGIAPEFLPHVFD
                     RFRQADQRTTRQHGGLGLGLAIARHLVELHGGTVHAASEGEGAGATFTVRLPVAPVYE
                     ADANAERVHPAARDTLPLYEYTDRLDGCNLLVVDDEQDTREMLRIGLGHCGA"
BASE COUNT         1113 a         2499 c         2252 g         1024 t
ORIGIN      
        1 cgcaccgttt cacccatcac ccgctgccaa acgcccgaag gcggtcggcc cgctggctgg
       61 tctgttttct gttgcacttg tcgtcgcctt gcccgcccgc gctgcgccga agcgtgtcgc
      121 cgccgaaccc ggcgcccagc cgttagctgg ctcgctgccc tgtagtgttc ggactttcct
      181 ctcccgcgcc cttacgacgc ggcagcgacc gcccgacctg ctctcatgca tggcgcgccg
      241 agtataacaa atcggcgttg aacgcgtaca gcggaattag aacgcgccca agcccaccca
      301 atgacattgg ccgacattta acgcgtgccg cgaccgctga acaccgtgta tgagatgccc
      361 cgcagacacg ccggttgcca cccgtgaagg gcacggattg gtcacgcggc caatgccctt
      421 cgccttttct gttccaaggt gggcgagggg ccgccgccgc gctctaaccg tccgccagag
      481 ccaattcgac cgcttcctcc ggcctcatcg cggctccttc ggctgcggcc agccccggcc
      541 tcctcttcgc ccgccgccgt tcgcgcgagc ttcatgcatc gttcgatcac ctgtaggcgg
      601 gccggcccca gacggagctt gattttttac ccgcaggccg gcggcggcgc cgtagaggac
      661 gagcgccaga cgcggttgcc gtgtggactg cgcgcaggct atcgcttcga gcgcggtcgc
      721 gatcccctgc gtatcgccga tctcctttaa ctcggcgagc gcgcccctct gccttgcgtg
      781 ggccgaggcc gtatcgccgc ggagcacggc gacttccccc tgcaggagcg ccgtccgggc
      841 cgcgagcggc ctgtgtccca gcagctcgaa taacgcgagg ctgtccgcca gacactcctc
      901 tgcccgttcg agccggcccg tctgcatcgc ggcgtcgccg aggttatgca ggcagaccgc
      961 ctccagatat ttatcttccg cacgccgcgc cgccgtcaag ctctccgtga agagcggcat
     1021 cgcttgcgag acctcgccca ggtcgagcag cggcctcgta gcgcgccgcc gcctccgcgt
     1081 cggcgctcct ctcgcccggc gggaaggaga gcggcgtgac ctccaggacg gtctcgccgg
     1141 ggatgttcag cgcctcgcgg ctcgtcgcga tgacctgtag cagccggctc gcgcgcagca
     1201 ggcgctcgca tagcccggcg caggcctcga tgacgtgctc gcagttgtcg aacacgagca
     1261 gcaggtgctt gtctttcaga tgctcgcgga gcgtctcctc gaccgcccgg ccagcctgct
     1321 ccgagaggcc gagcgccgtc atcagcgcct tcggcaccag cacctcgtcc gagatcggca
     1381 cgaaatccac cgcgcacacg ccgtgcgtgt aattctgcga gacgcccgcc gcgacctccg
     1441 tggccagtcg cgttttgccg atccccggcg gcccggtcag cgtaacgagc cgcgtccgcc
     1501 ggagcagctc aaggattctg gcgacctcgg cggtgcgccc gacgaagcgc gtcaaccgct
     1561 ccggcgccac gccggcgcgc ccaatatcgt cgatctcatc ggcgagtcgg agatcatcct
     1621 gcgcggccag atcaattaaa ctcaccgacg gcatcgagga aggcgtcccc tacacggaag
     1681 tcacggtgca ggtgagcgag gccgtccgcg gccaggtcgg cgcaggctac accttccgcc
     1741 agttcggcct cctgaagccg cggcggatga ggaacggcaa ggtcaacctc aacacctcgc
     1801 ccgcgggctg ggcgaagtac gcagtggacg aggaggtcgt cctcttcctc taccggcagg
     1861 ccgcctggac gggcctgcgc acgacggtgg gcctgggcca gggccagctc gaagtccgcg
     1921 ccggcaacat cgcgagtcag ttcgacaaca tgggtctttt cgagaatgtg acggtcgagc
     1981 acaacctgct gaacgattct gacaagcgcc tgctcggcac gaagaagggg gccgtgaacg
     2041 gggactcctt catgtctttc gtccgccgcg ccatcaatga caagtggatc gagaaaggaa
     2101 agatgcgcca tgcgaagaag taaatcgaca ctcctgtctt gcgccctcct cgccgtggcg
     2161 ttcgcctcca cggcggcccg ctcttcatgt tcgacgaggc gaaccgcatc ccgtaccgct
     2221 acgacgtttc gtcgccggtg ccggtctaca ccgatctcgg ccccttcgag atcgtcccca
     2281 gcccgcacgt gccgatcacc aacgagcgcg ccgacgagat cgtcgccttc gcgttcaagc
     2341 agtggacgga cgtgccgacc tctgcgttcc aggcgcaggt catcggcgac ttcgcatcaa
     2401 tcgggctgcg cgacgtgaag gacgcggcga ccgccgccct cgtcatcggc cctgaaaacg
     2461 gcggcggcat ccacgtcatc tacgacgccg acggctcgat cctgcgtgac ttcttcggca
     2521 cctcgccgtc cgtgctcggc atcgccagcc ccgaatgggc cgacgagacc acggggacgc
     2581 tcaccgagag ctgggccgtc atcaacgccc agcagcgctg ggtcggcgac caggagctgt
     2641 tgaactacgc cggcgtcttc acgcacgagt tcggccacgc gatcaacctc gcccactcgc
     2701 agaagaccgg cgccgtcgcc ttcttcggcg acgcgcgcgg ccccgcgggt tgtacgacgc
     2761 tgccctaccc gacgaccatg acgatcaacg acatcgagac gatgtacccg ttcatcaacc
     2821 cgcgccccac gacgggcagc ggcctcgcgc agtccaccgt ggatcgcacc gacgacatgg
     2881 ctgccgtctc cgacctctac ccggcccccg gctacccgca gacgcacggc tcgatcaagg
     2941 gcagggtgct ggcggcggac ggcaaggagg gcctcacggg cgtcaacgtc atcgcccgca
     3001 acgttgacca tcccttcgcg gacgccgtct tggcgatgtc gggcgactac acgcgcctcg
     3061 ccgtcggccc cgacggaagt ttcaccctga cgggcctgac gccgggcgcc cgctacgtcc
     3121 tctacaccga cgccatcgtg agcggcggct tcccgacccc gcagcccttc ttcatccccg
     3181 gccccgaaga gttcttcaac gccgagggcg agagcggcga cggcgtgacg gacaaccgct
     3241 gcgaggcggc gccggtgacg gctgtggcgg gcctgacgac ggaggccgac atcacgctca
     3301 acgccgtcaa gggcgcgccc aagttcatcc ccatcggccc cggcacgatc ccgaacagca
     3361 tcacgggcga cgggcgtgtc gtggggggca gcatccagca ggggccggtc ttccgctgga
     3421 cggaagaggg cggcgtggaa gtcctcggca ccgcccgcac cgtcaacggc acgatgtcgc
     3481 gcgacggctc cgtgttcggc ggcgagacag agcaagcgaa cggccttcgc gtggcgagtc
     3541 tgctgcgcgc gggcgccccc tgggagacgc tcccgctgcc ggcgggcatc accccctgcg
     3601 gcaccgacgc caccaccagc tacggcgtcg ccgaaatcgg gaaggccacg tccggggcgg
     3661 cctgggtctc cgggtgcagg gtgcgaccgt tcctctggac gccgacaggc ggcacccaaa
     3721 tgctgccgac gcccgcgaat gtctccaatt cgaggccgaa caccatctcg gcggacggct
     3781 cgacggtcgt cggctggtgg cagagcacgg gcaactcgtc ccggcgcggc gcgcgctggg
     3841 tcaacgggca gttcgaggag ttcagcaccc ccgagctgtc ggtcggcgag gcgcagaaca
     3901 cgaacccgga cggctcggtc atcgtcggcg cgggagccgg cccgcgacag gagccgtggc
     3961 gctggacccc agacggcggc gtgcagttcc tcggtcgtct cgcccccttc ggcttcgcgg
     4021 gggcgtcggc catcagcgac gacggccaga tcatcggggg cgtgggcggt ggcagtagca
     4081 tcagcccggc aacccgcatc tccttcctct ggatgcccaa gctggggacg gtcaatttcg
     4141 agcagttcct ccgggcgcag ggcacgtact tcggcgactc gatcctgaac gcgccgctgg
     4201 cgatgtcctc cgacggccaa gtcatcgtcc ggcttcggcc tcggcatcgg cggctgggtg
     4261 gtgcagatgg acaaggtgat cgtctgccac gcgccgccgg gcaaccccgc gaacgcccag
     4321 tcgctccgcg ttgcgttccc cgacagcctc ggcgcgcacc ttgagcacgg cgacacggtc
     4381 ggtctctgcg ccgacgaccg ggagggcgcc aactagaacg gcagcgattg ccggacgttc
     4441 gattcaatgt cgggcgcgcg ccacgcggca aacgtggcgc gcgcccgcca gcttcacgcc
     4501 gagggggcgc gcttttttat gccttgaccg aatccgaatc cttcacttac agtccatgta
     4561 ccaacccctt tccacacagg cacgggtttc gatcaacaag gaggaagatg agcgacgcta
     4621 aagaacgccc ggtcgtcgcc gacgccgcag gcgaatcggt cgcgcgactg cacgccgcgg
     4681 aggtcgcccc ttactggctc tctgcgctcg tcgagtcggc ggacgacgcc atcatcagca
     4741 agacgctcga cggcatcatc accagttgga acaacggcgc cgagcgcctc ttcggctaca
     4801 cggcggagga ggccgtcggt cggcccgtca cgatcctcat cccggaagac cacatcgacg
     4861 aggagccgac catcctcgcc cgcatcaggg cgggcgagcg catcgagcac tacgagacgg
     4921 tgcgcgtcac gaaggacggc aggcggctcg acatctcgct caccgtctcg ccgatcaggg
     4981 ccgccgacgg gcgcatcatc ggcgcctcga agatcgcgcg cgacatcacc gagcgcagac
     5041 ggatcgagcg ggcgctgaag gagggggccg agcgcctgaa cctggcgctc gcggcggcac
     5101 ggctcggcga ctggagttgg gacgcgcgga cggacgcggt cacgctctcc gagaccgccg
     5161 ccgacatctt cggcgtctcg ccggggccgg acttgacccg tgcgtagatg cgcgaactgg
     5221 ttcacgagga cgacgccgca cgagcgctcg gcgtcgtcca gcggtcggtc gaagagcgga
     5281 acgactacga catcgagtac cgcgtcaggc gaccggacgg ctcgcagcgg tgggtgatgt
     5341 cgaggggccg cgccgtctac gacgagggcg gcggactggt cgggatgctc ggcgtcgtgc
     5401 aggacatcac gcagcgcaag gagaccgaag agcggctgcg cgagcagtcc gagacgctgc
     5461 ggacggtgca cgacatcggc cagatcgtct ccgccgaact cgacctgcaa aaggtcgtgc
     5521 aggccgtcac cgacgcggcg accgagctga cgggcgcgcg cttcggctcc ttcttctaca
     5581 acgtgctcga cgagcgcggc gcctcctaca tgctctacac gctttcgggc gtccccgccg
     5641 aagccttctc tcacttcccg atgccgcgcg cgacagacct cttcggcccg accttccgcg
     5701 gcgagggcgt gctgcgcatc gacgacgtgc accgcgatcc gcgctacggt aagaactcgc
     5761 cctactacgg catgcccgag ggccacctcc ccgtggtcag ttatctggcc gtgcccgtcg
     5821 cctcgcgctc gggcgaggtg gtgggcggcc tcttcttcgg ccaccccgag ccgggcgtct
     5881 tcacggcgcg gcacgagcgc gtggtggccg gtctcgcggc gcaggccgcc gtggcgatgg
     5941 acaacgcgcg gctctacgag gcggccaagc gcgcgcgcgc cgaagccgaa gcggcggcgc
     6001 gggagaacga gcttctctac cgcgacgcgc gggaggcgaa ccgcctcaaa gacgagttcc
     6061 tcgccacggt ctcgcacgag ctgcggacgc ctctgacggc catcctcggc tggtcgcaca
     6121 tgctccgcct cgcacagctc gacgaggccg ccgccgtccg cgccttcgaa accatcgagc
     6181 gcaacgcgcg cgcgcaggcg cagctcattg acgaccttct ggacgtgtcg cgcgccatca
     6241 ccggcaagct ccgcatcgac gtgcgcgcgg tcgatccgaa ctccttcatc gaggcggcca
     6301 tcgaggccgt gcgcccggca gccgacgcca agaacgtgcg cgtccagaag atcatcgaca
     6361 cgggcgtcgt ctcggtctcg ggcgacccgg tgcgccttca gcaagtcgtc tggaacctgc
     6421 tgtcgaacgc catcaagttc acgccgaagg gcgggcgcgt gcaggtcagg ttgcagcgca
     6481 tcaactcgca catcgagatc gccgtcagcg acacgggcgc cggcatcgcg cccgagtttt
     6541 tgccgcacgt cttcgaccgc ttccgccagg ccgaccagcg gacgacgcgc cagcacggag
     6601 ggctgggcct cggcctcgcc atcgcgcgcc acctcgtcga gctgcacggc ggcaccgtgc
     6661 acgccgcatc ggaaggcgaa ggcgcgggcg cgaccttcac cgtgcggctg cccgtcgcgc
     6721 ccgtctacga agcggacgcc aacgccgagc gcgtccaccc ggcggcgcgc gacacgctgc
     6781 cgctctacga atacacagac cggctcgacg gctgcaacct gctcgtcgtg gacgacgagc
     6841 aggacacgcg cgagatgctg cggatcggcc tcggacattg cggggcgg
//
LOCUS       DBNGOH010001103         1896 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_351739, whole genome shotgun sequence.
ACCESSION   DBNGOH010001103 DBNGOH010000000
VERSION     DBNGOH010001103.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1896)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1896)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1896
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_351739"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..280
                     /locus_tag="AC159P_21505"
     CDS             <1..280
                     /locus_tag="AC159P_21505"
                     /inference="COORDINATES: protein motif:HMM:NF016895.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HIR3698077.1"
                     /translation="RNDAAAASVPAGGGYVVQAGSFNTASEANERVSALRAAGFEART
                     AAVELGRRGTWHRVYSGRFASREEASRHDRKLRASGAVGDTLVTPLQD"
     gene            365..1771
                     /locus_tag="AC159P_21510"
     CDS             365..1771
                     /locus_tag="AC159P_21510"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013661.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0016019 - peptidoglycan immune receptor
                     activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045087 - innate immune response [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein family
                     protein"
                     /protein_id="HIR3698078.1"
                     /translation="MSQTLPPDPTLYYSRVVESYAHFFDRPEVRLRFLHSTLAQQSAV
                     RERLDARLEKLAFVKRSRLYDQLLKIWLYHLIFRETRRLLPDVSRSGHGLLSLKSRAP
                     LSARVFFKCYQFRTVFFALSVAALALVSLGAYRGIAWSTRYVNAQLAERYGAGVVPST
                     AAAEARDHVFTREAAGSLPDYKAEKVWLVEEKDNYERYSNGARIVNDYATTTRPRSYF
                     VFKPGGERASEATETQPVGIVYHTSESHLDPFNADNSASIETRTRGLVEYVRKNSSYN
                     YVIDRFGQIYRVVRDEDAANHAGNSVWSDARGVYVGLNDSFLGVCFETTMAADGGERL
                     TEAQLVAGRLLTQVLRSRFQIEDANCVTHGLVSVNPSNMRITFHHDWARDFPFAAMGL
                     SDKYLVAPASVAEFGFTSDEEIKAKLGGQLWPGVAQAEQTFRQQATDANLDPETLRQR
                     SRERYRRQFDAQRDLRTE"
BASE COUNT          336 a          687 c          577 g          296 t
ORIGIN      
        1 gcgtaacgac gccgccgcgg cgagcgtgcc tgcgggcggc ggttacgtcg tgcaggcggg
       61 ttctttcaac accgcctccg aggctaacga gcgcgtgtcg gcgctacgcg cggcggggtt
      121 cgaggcgcgc acggcggcgg tcgaactcgg aaggcgcggg acttggcatc gagtctattc
      181 gggccgcttc gcctcgcgcg aagaggcgtc tcgacacgac cggaagctgc gtgcttcggg
      241 cgccgtcggc gacactctgg tcacgccgct acaggattga agggcgacgc cgaagtcgcc
      301 tcccacggca cgagttaacg ggcggcaccc tcgccgcaga gagtcaggcg ctagacgtta
      361 gatcatgagc cagacgttgc ccccagaccc gacgctttat tactcaaggg tggtcgaaag
      421 ctacgcgcac ttcttcgacc gccccgaagt gcgcctccgc ttcctgcaca gcacgctcgc
      481 ccagcagtcc gccgtccgcg agcgcctgga cgcgcggctc gaaaagctcg ccttcgtcaa
      541 acggtcgcgg ctctacgacc agctcctgaa aatctggctc taccacctca tcttccgcga
      601 gacgcgcagg ctcctgccgg acgtttcgcg gagcggccac gggctgctca gtctgaagag
      661 ccgggcaccg ctctcggccc gcgtcttctt caagtgctac cagttccgga cggtcttctt
      721 cgccttgagc gtggcggcgc tcgccctcgt ctcgctcggc gcctaccgcg gcatcgcttg
      781 gtctacgcgc tacgtcaacg cgcaactggc cgagcgttac ggcgcggggg tcgtcccctc
      841 caccgccgcc gccgaagcgc gcgaccacgt cttcacgcgc gaggccgcgg ggagccttcc
      901 cgactacaag gcggagaagg tctggctcgt cgaggagaag gataattacg agcgctacag
      961 caacggcgcg cgcatcgtca acgactacgc cacgacgact cggccccgca gctacttcgt
     1021 cttcaagccc ggcggcgagc gcgcgagcga ggcgaccgag acgcagcccg tcggcatcgt
     1081 ctaccacacc tccgagagcc acctcgaccc cttcaacgcg gacaacagcg cctccatcga
     1141 gacgcgcacg cgcggcctcg tcgagtacgt tcgcaagaac agctcctaca actacgtcat
     1201 cgaccgcttc ggccagattt accgcgtcgt gcgcgacgag gacgcggcga accacgccgg
     1261 caactccgtc tggtcggacg cgcgcggcgt ctacgtcggc ctcaacgaca gctttctcgg
     1321 cgtctgcttc gagacgacga tggcggcgga cggcggcgag cgcctgaccg aggcgcagct
     1381 cgtcgcgggc cgcctcctga cgcaagtcct gcgcagccgc ttccagatcg aagacgccaa
     1441 ctgcgtcacg cacggcctcg tctccgtcaa cccctccaac atgcgcatca ccttccacca
     1501 cgactgggcg cgcgacttcc ccttcgccgc gatgggcctc tccgacaagt atctggtggc
     1561 gcccgccagc gtcgccgagt tcggcttcac ctccgacgag gagatcaagg ccaagctcgg
     1621 cggccagctc tggcccggcg tcgcacaggc cgaacaaacc ttccgacaac aagcgacgga
     1681 cgccaacctc gaccccgaaa ccctccgcca acgctcacgc gaacgctacc gccgccagtt
     1741 cgacgcgcaa cgcgacctca gaaccgagta ggcagtaggc agcaggcagt aggcagtaaa
     1801 gcaagaggct tgagagtgaa tgtctcaagc ctcttgactt tattgcttga agggcggagc
     1861 ggcttgggac ttcatctctc aagcctctcc gccctt
//
LOCUS       DBNGOH010001104         2084 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_168216, whole genome shotgun sequence.
ACCESSION   DBNGOH010001104 DBNGOH010000000
VERSION     DBNGOH010001104.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2084)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2084)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2084
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_168216"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..893
                     /locus_tag="AC159P_21515"
     CDS             <1..893
                     /locus_tag="AC159P_21515"
                     /inference="COORDINATES: protein motif:HMM:NF012798.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HIR3698079.1"
                     /translation="DLLRDKQRIVLSFFDDTGKLPSEVEPTDVQAWLAALDEKGIRPG
                     TTYQHACLLSSFYTWAMKDAGIGQHVRRNPARLARPRAPKPYQTESVKALSDEEVSAL
                     VGAVRRRALSGDVVGKRDHALLLLYLATGLRRSEVISLRGQDVHVGGGGLVLAYRAKG
                     GDYRSREVREPQVRAALLDYLSAAGRLHVLKTDAPVWTRHDRAGKPGEALNSHCFVKN
                     LKKYAREAGVEDFHLHRTRHTYARIVSEFTGDITATQNALDHQSRSTTRVYVQRIAVK
                     RDLYSDEVSKRWGNDERVAD"
     gene            973..1995
                     /locus_tag="AC159P_21520"
     CDS             973..1995
                     /locus_tag="AC159P_21520"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR3698080.1"
                     /translation="MGGIPFSVLDLAPVRAGGTIADALSETLDLARHAEGWGYRRFWL
                     AEHHNMSGIASAATAILIEHVAHGTETIRVGSGGVMLPNHAPLVVAEQFGTLASLHPG
                     RIDLGRGRAPGTDQLTMRALRRTLSNPDEDFPRDVAELLSYFAPARPGQAVRAVPGEG
                     LRVPVWLLGSSLYSAQLAALLGLPFAFASHFAPDLLTQALDVYRSEFRPSESLAAPYA
                     MACVNAFAADTDEEARRLFTSLEQAFVNLRRGQPGPLPPPLDERGGRLSDVERAGAGH
                     VLRYSAVGSPETVRRWLESFVALTKVDEVMVTAMIYDHAARLRSFEIVAGVRDALNAE
                     REEVSQ"
BASE COUNT          330 a          704 c          725 g          325 t
ORIGIN      
        1 aggacttgct gcgagacaaa cagcgcatcg tgctctcttt cttcgacgac acggggaaac
       61 ttccttccga ggtcgaaccg actgacgtgc aggcgtggct cgccgcgttg gacgagaaag
      121 gcatcaggcc cggcaccact taccagcacg cctgcctgct ctcttccttc tacacgtggg
      181 cgatgaagga cgcggggatc ggccagcacg tccgccgcaa ccccgcacgg ctcgcccgac
      241 cgcgcgcgcc gaagccttac cagaccgagt cggtcaaggc gctgtcggac gaagaagtgt
      301 cggcgctcgt cggggccgtg cgccgccgtg cgctctccgg cgatgtcgtg gggaagaggg
      361 atcacgcgct gttgctactc tatctggcga cgggtttgcg caggagcgag gtcatctcgc
      421 tgcggggtca ggatgttcac gtcgggggcg gcgggctggt gctcgcctac cgcgccaagg
      481 gcggcgacta ccgcagccga gaggtgcgcg agccgcaggt cagggcggcg ctgttggact
      541 acctctccgc ggcggggcgg ctgcacgtgc tgaagaccga cgcgccggtg tggacgcggc
      601 acgaccgcgc gggcaagccg ggcgaggcgc tgaactcgca ctgcttcgtc aagaacctga
      661 agaagtacgc gcgcgaggcg ggggtcgaag acttccacct gcaccgcacg cggcacacct
      721 acgcgcgcat cgtctcggag ttcacgggcg acatcacggc gacgcagaac gcgctcgacc
      781 accagagccg ttcgacgacg cgcgtctacg tgcagcgcat cgccgtgaag agagacttgt
      841 acagcgacga ggtctcgaag cgctggggca acgacgagcg ggttgccgac tgacctgacg
      901 agcgacgacc ttgacgtgca agcagcgctt tgtcttcgac agcacgccgt gtgcaagggg
      961 tagaggtgag tcatgggggg aattcctttt tcggttctcg acctcgcgcc cgtgcgggcg
     1021 ggcggaacaa tcgccgacgc gctcagcgag acgctcgatc tggcgcggca cgccgaaggc
     1081 tggggctacc gccgcttctg gctggcagag caccacaaca tgtccggcat cgccagcgcg
     1141 gcgaccgcca tcctgatcga gcacgtcgcc cacggcaccg agacgattcg cgtcggctcc
     1201 ggcggggtga tgctgcccaa ccacgcgccg ctcgtcgtcg ccgagcagtt cggcacgctc
     1261 gcctcgctcc accccggcag gatcgacctc gggcgggggc gcgcgcccgg caccgaccaa
     1321 ctcacgatgc gcgcgctgcg ccgcacgctc tcgaacccgg acgaagactt cccgcgcgac
     1381 gtggccgagc tgctttcgta cttcgcgccc gcgaggccgg ggcaggcggt gcgcgccgtg
     1441 cccggcgagg gtttgcgcgt gcccgtctgg ctcctcgggt cgagcctcta cagcgcgcag
     1501 ctggcggccc tcttgggcct gccgttcgcc ttcgcctcgc acttcgcgcc cgacctgctg
     1561 acacaggcgc tcgacgttta caggagcgag ttccgcccct ccgaatcgct cgcggcgcct
     1621 tacgcgatgg cctgcgtcaa cgccttcgcg gctgacaccg acgaagaagc gcggcgcctc
     1681 ttcacgtcgc tcgaacaggc gttcgtcaac ctgcgccgcg ggcagccggg gccgctcccg
     1741 ccgccgctgg acgaacgggg cgggcgcctg tcggatgtcg agagggccgg tgccgggcac
     1801 gtgctccgct actcggcggt cggctcgcct gagacggtgc gccgttggct tgagtcgttc
     1861 gtcgcgctga cgaaggtgga cgaggtcatg gtgacggcga tgatctacga ccacgccgcg
     1921 cgcctccgct ctttcgagat cgtcgccgga gtgcgcgacg cgctgaacgc cgagcgcgag
     1981 gaggtgtcgc agtagcccct tcaagatgtg tgttacaaaa agaccgtggc aagactgtag
     2041 gggcagggca agtccgcggc ccgcggttat gattgattca aagt
//
LOCUS       DBNGOH010001105         4267 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4932904, whole genome shotgun sequence.
ACCESSION   DBNGOH010001105 DBNGOH010000000
VERSION     DBNGOH010001105.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4267)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4267)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4267
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4932904"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="AC159P_21525"
     CDS             <1..243
                     /locus_tag="AC159P_21525"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF020034.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3698081.1"
                     /translation="ADVQAATPEVIYLEGVYVHPSERGNGTARAYLSRLCRRLLPATR
                     TICLLANEENERAQRFYRTCGFKMRGVYDTIFLRRD"
     gene            complement(346..1668)
                     /gene="pepP"
                     /locus_tag="AC159P_21530"
     CDS             complement(346..1668)
                     /gene="pepP"
                     /locus_tag="AC159P_21530"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017521392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HIR3698082.1"
                     /translation="MPRPQLAELMRRMEPNSVAILPSAPEARRSNDTEYRYRQNSDFF
                     YVTGFSEPEAVAVIAPARDEERYTLFVRPRDPEREIWDGRRAGLEGAKELYGADASFT
                     VAEFNEKLADILNGARNLYYRLGDGNPELDRTVVGQIARMRAMGRRGVQAPHTIIDPG
                     TILHEMRLIKTDEEIALMQRAADIAAEGHREAMKSAKPGMKEYEVEALVEYVFRRSGA
                     AAPAYSSIVGAGANATILHYINNDAELKDGDLLLIDAGAEYEGYASDITRTFPVNGRF
                     TDAQRDIYELVLDCQEQCIRMVAPGVTFEEMHDRSVEILTEGMVRLGLLKGDPKKLIE
                     EGEYKKFYMHRLGHYLGMDVHDVGRYHDDGESRPVEAGVVMTVEPGLYIAADAEDIPD
                     KYRGIGVRIEDDVLVTKDGHRVLTDKAPKRVAEIEALMSQSKGSAAGG"
     gene            1858..3549
                     /locus_tag="AC159P_21535"
     CDS             1858..3549
                     /locus_tag="AC159P_21535"
                     /inference="COORDINATES: protein motif:HMM:NF012398.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIR3698083.1"
                     /translation="MATRISRPQTTDAPTGTRTGAETGTPTGTPTAAAREVVSYNPAT
                     GEEVGRVPLCTEADVRGAVARARAAQNEWRRLSFRERGEVVMRARAHVLAEMDEIASL
                     VSREMGKPAAESLSMEIVPTLDLMQFFARRSARLLKSERIDIGLYRLLGRSSCIEYRP
                     LGVVGIISPWNFPWAIPLGEVVMALMAGNTVVLKPSELPPLVALKIGDVFARAGLPAG
                     ALEIVTGDGSTGAALVEAGVDKLMFTGSVATGRRVAEAAARRLIPVVLELGGKDPMVV
                     LEDADIESASSAAVWGAFANSGQACASVERCYVHEKIADDFLRRVVEKTRALRQDAGE
                     GEGEGVDIGAMSSERQLHVVEEHVRDAIERGARVVTGGGRPKPVTSRPESVHATSRQG
                     FFHEPTVLTGVDHSMKVMREETFGPLLPLMTFRDEEEAVRLANDSPFGLTASVWTRDI
                     GRGQRLAARIEAGTVMVNEVLYTHGIAQTPWGGVKESGLGRTHGRLGLLEMVAPHHVH
                     TNRLARLPDVWWFGYTPQAAALFRSLARRFATGSALQTTLIMPQLLRRVLARRKK"
     gene            complement(3734..3865)
                     /locus_tag="AC159P_21540"
     CDS             complement(3734..3865)
                     /locus_tag="AC159P_21540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3698084.1"
                     /translation="MRAAFQKPLTSSRPPVVVLPASEAVGTTAPKMALLICCAVAPG"
     gene            complement(3867..4076)
                     /locus_tag="AC159P_21545"
     CDS             complement(3867..4076)
                     /locus_tag="AC159P_21545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HIR3698085.1"
                     /translation="MSTPLLYTLAGFTLIALAVYNSFTSIDMVVLGAIGIVAGLFLIV
                     CGQVRHHRRHHHRHRTRNAHRRIVT"
BASE COUNT          664 a         1416 c         1449 g          738 t
ORIGIN      
        1 gccgacgtgc aggccgccac gcccgaagtc atctacctcg aaggcgtcta cgtccacccg
       61 tccgagcgcg gcaacggcac ggcccgcgcc tacctcagca ggctctgccg ccgactgctc
      121 cccgccacgc gcaccatctg cctgctcgcc aacgaagaga acgagcgcgc gcagcgcttc
      181 taccgcacct gcggcttcaa gatgcgcggc gtctacgaca ccatcttcct ccgccgggac
      241 tgagaatcag gcacgaagtc ccgccgggga tgaaacagcc gaggccgcga gcacgtgaat
      301 gctcgcggcc tcgactgtct gttcgcgacc gttgacctct cgtgcttacc cgcccgccgc
      361 gcttcccttc gactgtgaca tcagagcctc gatctcggcg acgcgcttcg gggctttgtc
      421 tgtgaggaca cggtggccgt cctttgtgac gagcacgtcg tcttcgatgc ggacgccgat
      481 gccgcggtac ttgtcgggga tgtcctcggc gtccgcggcg atgtagaggc ccggctcgac
      541 cgtcatcacg acgcccgcct cgacggggcg cgactcgccg tcgtcgtggt agcggcccac
      601 gtcgtgcacg tccatgccga ggtagtggcc gaggcggtgc atgtagaact tcttgtactc
      661 gccctcctcg atgagcttct tcgggtcgcc cttgagcagg cccaaacgca ccatgccctc
      721 ggtcagaatc tcgaccgagc ggtcgtgcat ctcctcgaag gtgacgccgg gcgcgaccat
      781 ccgaatgcac tgctcctggc agtcgaggac gagttcgtag atgtcgcgct gcgcgtcggt
      841 gaagcgcccg ttgacgggga acgtgcgcgt gatgtcggag gcgtagcctt cgtactctgc
      901 gcccgcgtct atcaagagga ggtcgccgtc tttaagctcg gcgtcgttgt tgatgtagtg
      961 gaggatggtg gcgttcgcgc ccgcgccgac gattgaagag taggccggcg ccgcggcccc
     1021 gctgcggcgg aagacgtact cgaccagcgc ctcgacctcg tactccttca tgccgggctt
     1081 cgcactcttc atcgcctcgc ggtgcccctc cgcggcgatg tccgcggcgc gctgcatgag
     1141 ggcgatctcc tcgtcggtct tgatgaggcg catctcgtgg aggatggtgc cggggtcgat
     1201 gatggtgtgc ggcgcctgca cgccgcggcg gcccatcgcg cgcatccgcg cgatctggcc
     1261 caccacggtg cggtcgagtt cggggttgcc gtcgccgagc cggtaataga gattgcgcgc
     1321 gccgttcagg atgtcggcga gcttctcgtt gaactcggcg acggtgaacg aggcgtcggc
     1381 gccgtacagc tctttggcgc cctcaaggcc ggcgcgccgc ccgtcccaga tttcgcgctc
     1441 ggggtcgcgc gggcggacga agagcgtgta gcgctcctcg tccctcgcgg gcgcgatgac
     1501 ggcgacggct tcgggttcgc tgaagccggt gacgtagaag aagtccgagt tctggcggta
     1561 gcggtactct gtgtcgttcg agcggcgcgc ttcgggcgcg ctcggcagga tggcgaccga
     1621 gttcggctcc atgcgccgca tcagttccgc gagctgcggt cttggcatga cgtgtgtctc
     1681 tccctccgaa atgacgacgg gttgtgattg cttcgggcgc gtcgccgtcg atgacggctg
     1741 gccgtgaata ctcctcagcg cctcattgta actgacagcc gacgcggcgc gtcaaacgcg
     1801 cctccgggct tttaacctgc cgcgctttcg cgcgataatc ccgccgaggc gaaaccgatg
     1861 gcaactcgaa tctcccgacc gcagacgacc gacgcaccga ccggcacgcg aaccggcgca
     1921 gagaccggca cgccgaccgg cacgccgacc gcagccgcgc gtgaggtcgt ctcgtacaac
     1981 cccgcgacgg gcgaagaggt ggggcgcgtg ccgctctgca cggaggcgga cgtgcgcggc
     2041 gcggtcgcgc gtgcgcgcgc ggcgcaaaac gagtggcgcc ggctctcctt ccgcgagcgc
     2101 ggcgaggtgg tgatgcgcgc gcgcgcccac gtgctcgcgg agatggacga gatcgcttcg
     2161 ctcgtctcgc gcgagatggg gaagcctgcg gctgaaagcc tctcgatgga gatcgtgccg
     2221 acgctcgacc tgatgcagtt cttcgcgcgc cgctcggcgc ggctcctcaa gagcgagaga
     2281 atcgacatcg gcctgtatcg cctgctcggt cgctcgtcat gcatcgagta caggccgctc
     2341 ggcgtcgtcg gcatcatctc gccgtggaac ttcccgtggg cgataccgct cggcgaggtc
     2401 gtgatggccc tgatggcggg caatacggtc gtcctcaagc cgagcgagct gccgccgctc
     2461 gtcgctttga agatcggcga cgtgttcgcg cgcgccggct tgcccgcggg cgcgctcgaa
     2521 atcgtgacgg gcgacggctc gacgggcgcg gcgctggtcg aggcgggcgt cgataagctg
     2581 atgttcacgg gcagcgtcgc caccggcagg cgcgtggccg aggccgcggc gcggcgtctg
     2641 attccggtcg tgctcgaact cggcggcaaa gacccgatgg tcgtgctcga agacgcggac
     2701 atcgagtccg cttcgagcgc ggcggtgtgg ggcgccttcg ccaactccgg ccaggcgtgc
     2761 gcgtcggtcg agcgctgtta cgtgcacgag aagatcgcgg atgatttcct gcgccgcgtc
     2821 gtcgagaaga cgcgcgctct gcggcaggac gcgggcgagg gcgagggcga gggcgttgac
     2881 atcggcgcga tgtcgagcga gcggcagttg cacgtcgtcg aagagcacgt ccgcgacgca
     2941 atcgagcgcg gcgcgcgagt cgtcaccggc gggggccgcc cgaagcccgt gacttcccga
     3001 ccggagtccg ttcacgcgac ctctcgtcaa ggcttcttcc acgagccgac cgtgctcacc
     3061 ggcgtcgatc actcgatgaa ggtgatgcgc gaggagacgt tcgggccgct gctcccgttg
     3121 atgactttcc gcgacgagga agaggccgtt cgcctcgcca acgacagccc gttcggcctg
     3181 acggcgagcg tgtggacgcg cgacatcggg cgcggccagc gtctcgccgc gcgcatcgag
     3241 gcgggcaccg tgatggtcaa cgaagtcctc tacacgcacg gcatcgcgca gacgccgtgg
     3301 ggcggcgtca aagagagcgg cctcgggcgc acgcacgggc ggctcggcct gctggagatg
     3361 gtcgcgcccc accacgtcca caccaaccgc ctcgcacgcc tgcccgacgt atggtggttc
     3421 ggctacacgc cgcaggccgc cgcgctcttc cgctcgctcg cgcgccgctt cgccaccggc
     3481 tccgcgctgc aaaccacact catcatgccg cagcttctgc gccgcgttct cgcccgacgg
     3541 aagaagtagg cagtaggcag cgggcagcaa aggcaagagg cttgagacgt tgaatctcaa
     3601 gcctcttgcc tttgctgctt gacgaacgaa gaggcttgag actttacctc tcaagcatcg
     3661 tcactcttct gctttaactg cctactgcct actgcctacc gcttactgcc tgctgcctgc
     3721 tgcctactgc ccgctatccg ggcgcgaccg cgcagcagat caggagcgcc atcttcggcg
     3781 cggtcgtccc caccgcctcg ctcgcgggca agaccacgac gggcgggcgg ctcgacgtga
     3841 gcggcttctg gaaagccgcc cgcacgtcaa gtcacgattc ttcggtgggc gtttcgggtt
     3901 cggtgtcggt gatggtgcct gcggtggtgc cgaacctgcc cgcagacgat caggaacagc
     3961 ccggcgacga tgccgatggc gcccagcacc accatgtcaa tcgaagtgaa gctgttgtag
     4021 acggcgagcg cgatgagcgt gaaaccggcg agcgtgtaga ggagaggcgt ggacatcgag
     4081 gcactccttt tgcgagggag gcagagtagt ttacgagggg ccgcagagta acaggaggga
     4141 accgaaactt ccgtacgaag gcgtaccgtg ccgaccgcgc ttcgttcgtt gcgcggtcga
     4201 actttcgagc cataaaaaaa tctttcgtgc catcaaaaat ttgttatagt gcgcgttccc
     4261 ccgcgac
//
LOCUS       DBNGOH010001106         2613 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2734931, whole genome shotgun sequence.
ACCESSION   DBNGOH010001106 DBNGOH010000000
VERSION     DBNGOH010001106.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2613)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2613)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2613
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2734931"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(228..>2613)
                     /locus_tag="AC159P_21550"
     CDS             complement(228..>2613)
                     /locus_tag="AC159P_21550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014101121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LVIVD repeat-containing protein"
                     /protein_id="HIR3698086.1"
                     /translation="GDIVQNSMVVPGKWWRVKQTKDTLDEKSADYNWRSAYAKTVRID
                     DDKTETWGDVPADESKLAHPNNTMTCYTCHSSWMTSCFGCHLSMRANAKMPNRHNEDG
                     DSRNFTTYNYQVLRDDVFMLGKDGTVTGNRTAPVASRSAVLVSSQNQNREWIYSQQQT
                     ISSEGYSGQSFATHVPHTVRTKETMGCTDCHVSEKKDNNAWMAQVLLQGTNFVNFMGR
                     YVYVAAEHSLEVVTVTEHNEPQAVIGSTLHEAAYPDDYKKFEKGGRQLKVFFEHVGNP
                     EVLNVQLRGEYAYVAAGKGGLRVYDVAQIDHKGFSERITTAPVSRFGQKFYVKTKHAT
                     SVAAPSTLAVDPARSRLTSEGKFVAPFDKDFNVTPEWKEWAARWTAWQAMSAAEKQGK
                     PAPFLNEEQLIHPLYAFLYVTDSEEGLVLVNAATLLDGDPLNNYLKRALTFNPGGALN
                     GASNITIAGTFAYVTTPKGLSIIDLDNPLDPKLVKTVGEPELKHPHSIAVQFRYGFVV
                     DEEGLKVIDLTMTPGAGGRSVPRGEVVRGAEVALSDAHDVYVARTYAYVANGKEGLAI
                     IDVKEPERPRLYMKYNAEGEGHAGDKHHKKLDDTHQVKVAMTNASLFAYVADGHNGMR
                     VLQLTSPETEATYAGFSPAPRPRLIATFHTAGPAVAVSKGLDRDRAVDESGNQVAVFG
                     RRGARPFTLAEQQKLYLLDGRLRTVLDTDALTAPPPKKRDPFASSSTTSDDSAREGGK
                     PNDVDTSAGKAAPAANHSSGALKLTPTKLATGSVGALGFFAAFLGWRRRKRLRR"
BASE COUNT          382 a          772 c          945 g          514 t
ORIGIN      
        1 tcgaacccgc acgccttgcg gcacgggctt ctaagacccg cgcggctgcc attacgccac
       61 cccggcgcgc gtgttttgaa tgacaggtga gattaaaaga ttgactagtt aaagtctagc
      121 gacgagaagg aagggggaag agggtaaaag gggaaggggt gaaaaggaag acaagcggct
      181 ggcttgctcc ttttcacccc ttcccccttt accctcttcc ccgcgcgtta cctgcgaaga
      241 cgcttccgcc tgcgccagcc gaggaaggcg gcgaagaagc cgagggcgcc gacgctgccg
      301 gtggctagtt tcgtcggcgt gagtttgagc gcgcccgacg aatgattcgc ggcaggcgcg
      361 gctttgccag cggacgtgtc aacatcgttc ggcttgccgc cctcgcgggc cgagtcgtcc
      421 gaagttgttg acgacgacgc gaagggatcg cgttttttgg gcggcggcgc ggtgagcgcg
      481 tcggtgtcga ggacggtgcg caggcgaccg tcgagcaggt agagcttctg ttgctcggcg
      541 agcgtgaagg ggcgcgcgcc gcgccgcccg aagacggcga cctgattgcc gctctcgtcc
      601 acggcgcggt cgcggtcgag tcccttcgag acggcgaccg cggggccggc ggtgtggaag
      661 gtggcgatca ggcgcgggcg tggcgcgggg ctgaagcccg cgtaagtggc ctcggtctcg
      721 ggcgaagtga gttgcaggac gcgcatgccg ttgtggccgt cggcgacgta ggcgaagagg
      781 ctcgcgttcg tcatggcgac cttgacctga tgcgtgtcgt cgagcttctt gtggtgcttg
      841 tcgcccgcgt ggccctcgcc ctcggcgttg tacttcatgt agaggcgcgg gcgctccggc
      901 tccttcacgt cgatgatggc cagcccctcc ttgccgttgg cgacgtaggc gtaggtgcgc
      961 gcgacgtaaa cgtcgtgcgc gtcggagagc gcgacttcgg cgccgcggac gacttcgccg
     1021 cgcgggacgg agcggccccc ggcgcccggc gtcatcgtca ggtcgatgac cttcaggccc
     1081 tcttcatcga cgacgaagcc gtagcggaac tggacggcga tggagtgcgg gtgcttcagc
     1141 tcaggctcgc cgacggtctt gacgagcttc gggtcgagcg ggttgtcgag gtcgatgatg
     1201 gagagacctt tgggcgtcgt gacgtaggcg aaggtgcctg cgatggtgat gttgctcgcc
     1261 ccgttgagcg cgccgccggg gttgaaggtg agcgcgcgct tcaaatagtt gttgagcgga
     1321 tcgccgtcga gcagggtcgc cgcgttgacg aggacgagtc cctcctcgct gtctgtgacg
     1381 tagaggaagg cgtagagcgg gtggatcagt tgctcttcat tgaggaaggg cgcgggcttg
     1441 ccctgcttct cggcggcgct catcgcctgc cacgccgtcc aacgcgcggc ccactccttc
     1501 cactcgggcg tgacgttgaa gtccttgtcg aagggcgcga cgaacttgcc ctcgctcgtc
     1561 aggcgcgagc gcgccggatc gaccgccagc gtcgagggcg cggcgaccga ggtcgcgtgc
     1621 ttggtcttca catagaactt ctggccgaag cgcgagacgg gcgcggtcgt gatgcgctcg
     1681 gagaagccct tgtggtcgat ctgcgccacg tcgtagacgc gcaggccgcc cttgccggcg
     1741 gcgacgtagg cgtactcgcc gcgcagctgg acgttcagca cctcggggtt gccgacgtgc
     1801 tcgaagaaga ccttgagctg gcgtccgccc ttctcgaact tcttgtagtc gtcggggtag
     1861 gccgcctcgt ggagcgtgct gccgatgacg gcctgcggct cgttgtgctc tgtgacggtg
     1921 acgacttcga gcgagtgctc ggcggcgacg tagacgtagc gccccatgaa gttgacgaag
     1981 ttcgtgccct gaagcagcac ttgcgccatc cacgcgttgt tgtccttctt ctcggagacg
     2041 tggcagtccg tgcagcccat cgtctctttg gtgcggacgg tgtgcgggac gtgcgtggcg
     2101 aacgactgtc cgctgtagcc ctccgaggag atggtctgct gctgcgagta aatccactcg
     2161 cggttctggt tctgcgagct gacgagcacc gccgagcgcg aggcgacggg cgccgtgcgg
     2221 ttgccggtga cggtgccgtc cttgccgagc atgaacacgt cgtcgcgcag gacttgatag
     2281 ttgtaggtgg tgaagttgcg cgagtcgccg tcctcgttgt ggcggttggg catcttggcg
     2341 ttggcgcgca tcgagaggtg acagccgaag cagctcgtca tccacgaaga gtgacaggtg
     2401 tagcacgtca tcgtgttgtt cgggtgcgcg agcttcgact cgtccgccgg cacgtcgccc
     2461 cacgtctcgg tcttgtcgtc gtcgatacgg acggtcttgg cgtaggccga gcgccagttg
     2521 tagtcggcgc tcttctcgtc gagcgtgtct ttggtctgct tgacgcgcca ccacttgccg
     2581 gggacgacca tcgagttctg gacgatgtcg ccg
//
LOCUS       DBNGOH010001107         2978 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2183344, whole genome shotgun sequence.
ACCESSION   DBNGOH010001107 DBNGOH010000000
VERSION     DBNGOH010001107.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2978)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2978)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2978
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2183344"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..747
                     /locus_tag="AC159P_21555"
     CDS             <1..747
                     /locus_tag="AC159P_21555"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008318039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HIR3698087.1"
                     /translation="DVAFAESRIRPETIAAEDVLHDLGVFSMISSDSQAMGRVGEIIL
                     RTWQTAHKMKVQRGWLAPDAGRAQPVEADSRNDNWRAKRYVAKYTINPAIAHGVAHHV
                     GSVEPGKLADLVVWKPAFFGVKPELVVKGGLIAWGVMGDANASIPTPQPVLYRPMFGA
                     FGDANKRTSLTFVSRAAADSDLARRLGLRKTVVAVEGCRRVGKADMVHNSATPEIEVD
                     PETYEVRADGELLACEPAEVLPMAQRYFLF"
     gene            753..1448
                     /gene="ureG"
                     /locus_tag="AC159P_21560"
     CDS             753..1448
                     /gene="ureG"
                     /locus_tag="AC159P_21560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreG"
                     /protein_id="HIR3698088.1"
                     /translation="MPHEHRERAFRVGVAGPVGSGKTALVDALSRELWPRRSLAVVTN
                     DIYTKEDAEFLIRQGTLPRERVRGVETGGCPHAAIREDASMNLEAIADFEHALPGLEL
                     VFVESGGDNLAAAFSPELVDVFVYVIDVSGGDKIPRKGGPGVTRSDLLVINKTDLAPH
                     VGASLEVMERDARRMRQERPFLFTHLKTGEGLREVVGWLEAQLSVPHAERRGVIDAHA
                     PYAGRPHTHDHTH"
     gene            1486..1983
                     /locus_tag="AC159P_21565"
     CDS             1486..1983
                     /locus_tag="AC159P_21565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3698089.1"
                     /translation="MRKFVAGVSLWLFVVALSLQMGAGLYEMRVVTPLWSSSPPASVR
                     AWNADGARTIEPRARFWRYCTAAVGLTAALALVAGLTKRGRGRKWLVASSATAVVLVL
                     LTYLYFAPALALLLERRGAGLSDEQIIERVSSWLWLGRVRAAAYVLAWLAALKALSVA
                     GEREV"
     gene            2016..2402
                     /locus_tag="AC159P_21570"
     CDS             2016..2402
                     /locus_tag="AC159P_21570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014321766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HIR3698090.1"
                     /translation="MEIRTVHTDNAPTPAGHYSQAVVYGGLVYVAGQLAIDPRTGERK
                     LGSIEEQTEQALMNVAEILKAAGSDLSRVLKMTVYVADIGLWGAVNETYARLLGEHRP
                     ARAVIPTRDLHHGFLVEIDAVAATRE"
     gene            2663..>2978
                     /locus_tag="AC159P_21575"
     CDS             2663..>2978
                     /locus_tag="AC159P_21575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR3698091.1"
                     /translation="MTGDLLLAQLFGHVRPTATDNKKATDYELAQAASKGDMPAFEEL
                     YERHSRRVYSLCLRMTANTAEAEDLAQEAFIQLFRKIGSFRGESAFTTWLHRLTVNQV
                     LMH"
BASE COUNT          487 a          988 c         1057 g          446 t
ORIGIN      
        1 gacgtggcct tcgccgagag ccgcatccgc cccgagacca tcgccgccga agacgtgctc
       61 cacgacctcg gcgtcttcag catgatctcc agcgactcgc aggcgatggg gcgcgtgggc
      121 gagatcatcc tgcggacctg gcagaccgcg cacaagatga aggtgcagcg tggctggctg
      181 gcgccggacg ccggacgcgc gcaacccgtg gaagcggatt cgcgcaacga caactggcgc
      241 gccaagcgct acgtcgccaa gtacacgatc aacccggcca tcgcgcacgg cgtcgcgcac
      301 cacgtcggct ccgtcgagcc gggcaagctc gccgacctcg tcgtctggaa gcccgccttc
      361 ttcggcgtca agcccgagct ggtcgtcaag ggcggcctca tcgcgtgggg cgtgatgggc
      421 gacgccaacg cctcgatccc gacgccgcag cccgtgctct atcgcccgat gttcggcgcg
      481 ttcggcgacg cgaacaaacg gacgagcctc accttcgtct cgcgcgcggc ggcggactcc
      541 gacctcgcgc ggcggctcgg cctgcggaag acggtcgtcg cggtcgaagg ctgccgccgc
      601 gtcggcaagg ccgacatggt tcacaactcc gcgacgccgg agatcgaggt tgaccccgag
      661 acctacgagg tgcgcgccga cggcgagctg ctcgcgtgcg agccggcgga agtcttgccg
      721 atggcgcagc ggtattttct cttctgacga acatgccaca cgaacatcga gagcgtgcgt
      781 ttcgcgtcgg cgtcgccggg ccggtcggca gcggcaagac ggctctggtg gacgcgctga
      841 gcagggagct gtggccgcgc cgcagtctgg ccgtcgtgac caacgacatc tacacgaagg
      901 aggacgcgga gttcctcatc cgccagggca cgctgccgcg agagcgcgtg cgcggcgtcg
      961 agacgggcgg gtgcccgcat gcggcgatcc gcgaggacgc gtcgatgaac ctcgaagcca
     1021 tcgccgactt cgagcacgcg ctgccggggc tggagctggt cttcgtcgag agcggcggcg
     1081 acaacctcgc ggcggccttc agccccgagc tggtcgatgt cttcgtctac gtgatcgacg
     1141 tgtcgggcgg cgacaagatt ccgcgcaaag gggggccggg cgtcacgcgc tcggacttgc
     1201 tcgtcatcaa caagacggac ctcgcgccgc acgtcggggc ctcgctcgaa gtgatggagc
     1261 gcgacgcgcg gcggatgcgg caggagcgcc ccttcctctt cacccacctg aagaccggcg
     1321 aggggctgcg cgaggtggtg ggctggctcg aagcgcagct cagcgtcccc cacgccgagc
     1381 ggcgcggcgt catcgacgcc cacgccccct acgccggtcg cccgcacacg cacgaccaca
     1441 cgcattgacg acaggtaaag tggccggccg gggtcgcagt ccttcatgag aaagttcgtc
     1501 gcgggagttt cgctgtggct cttcgtcgtc gcgctcagct tgcagatggg cgcggggctg
     1561 tacgagatgc gcgtggtgac gccgctctgg tctagctcgc cgcccgcgtc ggtgcgcgcg
     1621 tggaacgcgg acggcgcccg cacgattgag ccgcgcgcga ggttctggcg ctactgcacg
     1681 gcggcggtcg ggctgacggc ggcgctcgcg ctcgtcgccg gtttgacgaa gcgcgggaga
     1741 ggacgcaagt ggctcgtcgc ttcgtcggcg acggcggtcg tgctggtgct cttgacgtac
     1801 ctctacttcg cgccggcgct cgcgctgttg ttggagcggc gcggcgcggg tctgagtgac
     1861 gaacagataa tcgagcgcgt aagcagttgg ctgtggctgg gccgcgtgcg cgcggcggcg
     1921 tacgtgctgg cgtggctggc ggcgctgaag gctttgagcg tggcgggcga gcgggaggtt
     1981 tgaccgcccc cgcgcgcggc gttatcttcg gcggcatgga gatcaggacg gttcacacgg
     2041 acaacgcgcc gacgcccgcg ggccactact cgcaggccgt cgtctacggc gggctggtgt
     2101 acgtcgccgg gcaactggcc atcgacccgc ggacgggcga gcgcaagctc ggctcgatag
     2161 aggagcagac cgagcaggcg ctcatgaacg tggccgagat tctgaaggcc gcgggcagcg
     2221 acctcagccg cgtgctgaag atgacggtct acgtcgcgga catcggcctg tggggcgcgg
     2281 tcaacgagac ttacgcgcgc ctgctcggcg agcaccggcc cgcgcgcgcc gtcatcccga
     2341 ccagagattt gcaccacggc ttcctcgtcg agatcgacgc cgtcgccgcg acgcgcgagt
     2401 gagcgcccgc ccggtcgggc cttcgcgcgc gaacgggtgt ggtagaatcg ccgccgccgc
     2461 gaaggaaata gcttgaattt caggcgggaa agggcaacga cgcgccgcgc acgacgcatc
     2521 tgacgacgcg ggaacccgtc cggcgaaggg ccgcgtcaat caggctacaa gcaagtcacg
     2581 ggcatccgcg cgccgacata cgcagttcga gttggtcgct tccggaatgg cggtcgaaga
     2641 agtggagagg cacgaacacg agatgaccgg tgaccttctg ctcgcccaac tgttcggaca
     2701 cgtgaggcca accgcgaccg acaacaagaa agccacggac tacgagctgg ctcaggcggc
     2761 ttccaaaggc gacatgccgg ccttcgaaga gctgtacgag cggcacagcc gccgcgtcta
     2821 ctcgctctgc ctgcggatga cggccaacac cgccgaggcc gaagacctcg cgcaggaagc
     2881 cttcattcaa ctgttccgca agatcggctc tttccgcggc gagtcggcct tcacgacgtg
     2941 gctccatcgt ttgacggtca atcaggtgtt gatgcact
//
LOCUS       DBNGOH010001108         3145 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1086850, whole genome shotgun sequence.
ACCESSION   DBNGOH010001108 DBNGOH010000000
VERSION     DBNGOH010001108.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3145)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3145)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3145
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1086850"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..314
                     /gene="rnpB"
                     /locus_tag="AC159P_21580"
     ncRNA           <1..314
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="AC159P_21580"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            410..685
                     /locus_tag="AC159P_21585"
     CDS             410..685
                     /locus_tag="AC159P_21585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="FitA-like ribbon-helix-helix domain-containing
                     protein"
                     /protein_id="HIR3698092.1"
                     /translation="MANRKGVFIGAYVPADLKRALQQRAASEHRTLSQEITRILEESV
                     KGRRLPPGVIDRRAPGSPLRRRNSDPPERRRAGDLPYVPGAPDTEDE"
     gene            814..1596
                     /locus_tag="AC159P_21590"
     CDS             814..1596
                     /locus_tag="AC159P_21590"
                     /inference="COORDINATES: protein motif:HMM:NF025910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3698093.1"
                     /translation="MNTQKLIFLSVGVALGFAAGFWLANGINRGEQEKLRAELTRLRA
                     EAARSEQGPGARGEQAGGDGAIPTLTDAQLRAAVERADASPSDAKLQQMSGQALYVYA
                     REKGNAAIIPDVVRILRRAHDIEPKNLTTTTMLGDALYLLAQTTGETGHLAEARKFYG
                     QVLAQKPDEVYVRTSLGLTYFYDKPSDPRAAIREYRKALKIDPRHEATLQGMVAALAA
                     AGEVPEAEASLRLLEEVNASNPEIANLRAQIAQQKNAGGERD"
     gene            1599..1934
                     /locus_tag="AC159P_21595"
     CDS             1599..1934
                     /locus_tag="AC159P_21595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3698094.1"
                     /translation="MKYVIVITLFAAVALFVYWRLRPYLKMARSVLGFVRDVQRVSTG
                     ARPEQATPPAQQQGGGAAQKLVRCSSCGTWLPSTRALALRGSNANYCSQDCVERSIAG
                     EAKPKAVRR"
     gene            complement(1965..>3145)
                     /locus_tag="AC159P_21600"
     CDS             complement(1965..>3145)
                     /locus_tag="AC159P_21600"
                     /inference="COORDINATES: protein motif:HMM:NF023128.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3698095.1"
                     /translation="PLCITLAVMLQIFLLPLLGAIADYSNLKKRLMAVFCYTGASATA
                     LMYFVADGRYLLGGLLFLVAMLSFNAAIVLYNSYLPQITTEDRRDKVSSRGFALGYLG
                     GGILLLLNSLFINAAPRLGLAETTAVRLSLFSAGIWWGGFALITFLTLRSHNAARPLP
                     PGKGILTVGFSELGRTFRELRRLKHTFRYLLAYLFFIDGVQTIIGMAGVFLAQELFVS
                     RGAATDRSFLIGIFLMVQFMAIFGALLFERVAAFVGTKRAIMASLVIWSGIVIYAYAF
                     LQTTGQAWWMAAVIAVVLGGTQALSRSLFSRMIPAGREASFFGLYEISERASWMGMGV
                     FTIVVNQTGSYRQAILFLIFFFAVGLFILLFTDTDRAIHEAGNALPEEVEQADTPVSV
                     "
BASE COUNT          588 a         1029 c         1106 g          422 t
ORIGIN      
        1 agcgggccgt ccgccttcgg gcgcgcgcgg cagcgggtga tgggtgaaat ggtgcggccc
       61 cgcggggctt aaagtaagag cgcaccggcg cgcttggtga caggcgcggc caggcaaact
      121 cctcgcagtg caagaccaaa tagggaggcg tcacacaggg ctgcccgccc gaagccgttt
      181 cttcggaaac ggctacgacc tccgggtagg tcgcttgagc cgtccggcga cggacggcct
      241 agatgaatga tcgccgcccc ggcgcgcgca agccgtcggg gagacagaat tcggcttaca
      301 gacctgctct cgacatgcac ggcacacatc ttaagctcta ccaaacggcg cggcgccccg
      361 acttcctctg ccaacacccg cgccctgctc atcaggagac acgagagaca tggcaaaccg
      421 gaagggggtc ttcatcgggg cctacgtccc ggctgatctc aagcgggcct tgcagcagcg
      481 cgcggccagc gagcaccgca cgctctcgca ggagattacg cgcatcctcg aagagtcggt
      541 gaaaggcagg cgcctgccgc ccggcgtcat cgaccgccgc gcgcccggct ccccgctgcg
      601 ccgccgcaac tccgacccgc ccgagcgccg ccgcgcgggc gatctccctt acgtccccgg
      661 cgcgcccgac acggaagacg aatagaacgt gtagagagac gctgatcgaa ggcccttgag
      721 tcgtcctcac ggcgcggctc aagggccttc gcgtttcctg acggcccgcg cgttctgcta
      781 aacttcggcg gcaggcgcgc tctttaatct ctcgtgaaca cacagaagct catcttcctc
      841 tcggtcggcg tcgcgctcgg tttcgccgcg gggttctggc tggcgaacgg catcaaccgc
      901 ggcgagcagg agaagctgcg ggcggagctg acgcgcctgc gcgccgaggc ggcgcggagc
      961 gagcaggggc cgggcgcgcg gggagagcag gcgggcggcg acggggccat cccgacactg
     1021 acggacgcgc agctccgcgc ggcggtcgag cgggcggacg ccagcccctc ggacgcgaag
     1081 ttgcagcaga tgtcgggcca ggcgctctac gtctacgcgc gcgagaaggg taacgccgcg
     1141 atcatcccgg acgtggtgcg catcctccgg cgcgcgcacg acatcgagcc gaagaacctg
     1201 acgacgacca cgatgcttgg cgacgcgctc tacctgctcg cgcagacgac gggcgagacc
     1261 ggccatctcg cggaggcgcg caagttctac gggcaggtgc tcgcgcaaaa gccggacgag
     1321 gtctacgtcc gcacgagcct cggcctgacc tacttctacg acaagccgtc cgacccgcgc
     1381 gcggccatcc gcgagtaccg caaggcgctc aagatcgacc cgcgccacga ggcgacgttg
     1441 caggggatgg tggccgcgct cgccgcggcg ggcgaggtcc cggaggcgga ggcgagcctg
     1501 cgcctgctcg aagaggtcaa cgcctcgaac ccggagatcg ccaacctgcg cgcgcagata
     1561 gcacagcaga agaacgcggg cggggagagg gattgatctt gaaatacgtc atcgtcatca
     1621 cgctgttcgc cgccgtcgcg ctgttcgtct actggcgcct gcgcccctac ctgaagatgg
     1681 cgcgcagcgt gctcggcttc gtgcgcgacg tgcagcgcgt gagcacgggc gcgcgcccgg
     1741 agcaggcgac gccgccggcg cagcaacagg gcggcggggc ggcgcagaag ctcgtgcgct
     1801 gttcgtcctg cggcacgtgg ctcccctcga cgcgcgccct cgcgctgcgc gggtcgaacg
     1861 ccaactactg ctcgcaggac tgcgtcgagc gctcgatcgc cggcgaagcg aagccgaagg
     1921 ccgtccggcg ctgacgcgga tgcgagaggc cgcaccggac ttcttcaaac gctaacgggc
     1981 gtatccgctt gttcgacctc ttccggcagg gcgttgcccg cttcgtggat ggcgcggtcg
     2041 gtgtccgtga agaggaggat gaagaggccg acggcgaaga agaagatgag gaagaggatg
     2101 gcctgtcggt aggagccggt ctggttgacg acgatggtga agacgcccat gcccatccac
     2161 gacgcgcgct cggagatttc gtagaggccg aagaaggaag cctcgcgccc cgccgggatc
     2221 atccgcgaga agagcgagcg cgagagcgcc tgcgtcccgc ccagcacgac cgcgatcacc
     2281 gccgccatcc accacgcctg ccccgtcgtc tgcaagaacg cgtaagcgta aatcacgatc
     2341 ccggaccaga tgacgagcga cgccatgatc gcgcgcttcg tcccgacgaa ggcggcgacg
     2401 cgctcgaaca gcagcgcgcc gaagatcgcc atgaactgca ccatcaggaa gatgccgatg
     2461 aggaacgagc ggtcggtcgc cgcgccgcgc gagacgaaca gctcctgcgc gaggaagacg
     2521 cccgccatgc cgatgatggt ctgcacgccg tcgatgaaga agaggtaagc gagcaggtag
     2581 cggaacgtgt gtttgagcct gcgcagctcg cggaaggtgc gcccgagttc cgagaagccg
     2641 acggtcagga tgcctttgcc cggcggcagc ggtcgcgccg cgttgtgcga gcgcagcgtg
     2701 aggaaggtga tgagcgcgaa gccgccccac cagatgcccg ccgagaacag agagaggcgc
     2761 acggcggtcg tctccgcgag tccgagccgc ggcgcggcgt tgatgaagag cgagttcagc
     2821 aggagcagga tgccgccgcc gaggtagccg agcgcgaagc cgcggctcga aactttgtcg
     2881 cgccggtcct cggtcgtgat ctgcggaaga tatgagttgt agaggacgat ggccgcgttg
     2941 aagctcagca tggcgacgag gaagagcagc ccgccgagta gataacgccc gtcggcgacg
     3001 aagtacatca gcgcggtcgc actcgcgccc gtgtagcaga agacggccat caaacgcttc
     3061 ttcaaattcg agtagtcggc gatggcgccg aggagcggca gcaggaatat ctggagcatc
     3121 acggcgagcg tgatgcagag cgggt
//
LOCUS       DBNGOH010001109         2734 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3468278, whole genome shotgun sequence.
ACCESSION   DBNGOH010001109 DBNGOH010000000
VERSION     DBNGOH010001109.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2734)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2734)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2734
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3468278"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..550
                     /locus_tag="AC159P_21605"
     CDS             <1..550
                     /locus_tag="AC159P_21605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017462105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR3698096.1"
                     /translation="DRLFAAVAEKFGKIDVLFVNAGIGKFAPVEAVTEELFDQIMDIN
                     FKGAYFTIQKALPLLNDDASVILNTSINAHIGMPNSSVYAASKAALITLARTLSAELV
                     GRGIRVNAVSPGPVATPIYGRLGLQPEELRETARNIQEQVPLKRFGRPEEIAKTVLFL
                     ASSDSSFLLGTEIVADGGMSQL"
     gene            575..811
                     /locus_tag="AC159P_21610"
     CDS             575..811
                     /locus_tag="AC159P_21610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3698097.1"
                     /translation="MFTGKRVKVIKRAGRREAPAARTEKGSAVAGVSVAGARRETVAV
                     VEGWVRDLRRRKVEEAARGFESLFGGAAHAAPRG"
     gene            835..1791
                     /locus_tag="AC159P_21615"
     CDS             835..1791
                     /locus_tag="AC159P_21615"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007906822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase family protein"
                     /protein_id="HIR3698098.1"
                     /translation="MKAIRFHEYGDADVLRLEEVEKPSLAAGSVLIKTAVAGINYADT
                     MLRKGTYLTRPPLPFTPGFEVAGYVEAVGEGVEGLRVGERVMAKMSGGGYAEYAAVKA
                     EHVTHVPEGLEFGAATALLAQGLTALGLLRDLKAGQSILVHAAAGGVGSLLVQLAKLR
                     GAHIIGTASTAEKLGLVKALGADAAIDYTASDWPEQVKAATPRGAGVDLLIEMVGGKV
                     GAQNVKCLAPGATMIVYGAASGEDFSISALGLLGRNLTVRGYTLYAESETTLAEFTRE
                     LMSLVEEGRLKVTVEVFPLARAADAHRAVEGRRTTGKVVLRV"
     gene            1813..2445
                     /locus_tag="AC159P_21620"
     CDS             1813..2445
                     /locus_tag="AC159P_21620"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009285848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0052851 - ferric-chelate reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent F420 reductase"
                     /protein_id="HIR3698099.1"
                     /translation="MKIGIIGSGNIGANAARLFVRAGHQVALSNSRGGEGLEELVAEL
                     GPRARATTIEDAARFGEVSLVAIPFGNFRELPAEAFRGKIVLDAGNYYPQRDGLFAEL
                     DNGQTTSSELLASHLSGARLVKGFNTIWFEHLATQGDTNLPLQERRAIFIAGDDTQAK
                     ETIARLIEEIGYASFDTGPLREGGRLQQPGTPIYNRVLTAKQAADVLASA"
     gene            2536..>2734
                     /locus_tag="AC159P_21625"
     CDS             2536..>2734
                     /locus_tag="AC159P_21625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3698100.1"
                     /translation="MQKILYVGMDVHQACVVIVVLNDAGKTVSEAIIETKTETVRAFI
                     RGLRGEVRVTFEEGAQAAWLYE"
BASE COUNT          520 a          841 c          936 g          437 t
ORIGIN      
        1 cgaccggctg ttcgccgccg tcgcggagaa gttcgggaag atcgacgtgc tcttcgtcaa
       61 cgccgggatc ggcaagttcg cgccggtcga ggcggtgacc gaagagctgt tcgaccagat
      121 catggacatc aacttcaagg gcgcgtactt cacgatccag aaggcgctgc cgctcctgaa
      181 cgacgacgcg tcggtcatcc tcaacacgtc catcaacgcg cacatcggga tgcccaactc
      241 aagcgtctac gcggcgagca aggcggcgct catcacgctg gcgcgcacgc tctcggctga
      301 gctggtgggc cgcggcatcc gcgtcaacgc cgtcagcccc ggccccgtcg ccacgccgat
      361 ctacgggcgg ctcgggctgc aacccgaaga gttgcgggag acggcgcgga acatacagga
      421 gcaggtgcct ttgaagcgtt tcgggcggcc cgaggagatc gcgaagaccg tgctctttct
      481 ggccagctcc gattcttctt tcctgctggg gacggagatc gtcgccgacg gcggcatgag
      541 tcagctctga ggcggaggcg gaagaagggt gtttatgttt accggaaaac gtgtcaaggt
      601 catcaagcgg gcggggcgtc gagaggcgcc cgctgcgcgc acggagaagg ggagcgccgt
      661 cgcaggtgtt tccgtcgcgg gcgcgaggcg cgagacggtc gcggtcgtcg agggttgggt
      721 tcgggacttg cgtcgcagga aggtcgagga agcggcgcgc gggttcgaga gcctgttcgg
      781 cggggctgct cacgcggccc cgcgcggctg aggaaacaca cgggaggagg gaaaatgaag
      841 gccatcaggt ttcatgaata cggcgacgcg gacgtgctgc gtcttgaaga ggtggagaag
      901 ccgtcgctgg ccgccggctc ggtgctcatc aagaccgcag tcgccggcat caactacgcc
      961 gacacgatgc tgcgcaaagg gacttacctg acgcgcccgc cgctcccgtt cacgcccggc
     1021 ttcgaggtcg cagggtacgt cgaggccgtc ggcgagggcg tcgagggttt gcgcgtcggc
     1081 gagcgtgtga tggcgaagat gagcggcggc ggctacgccg aatacgccgc cgtcaaggca
     1141 gagcacgtca cacacgtccc cgaaggcttg gaattcggcg cggcgacggc gctgctcgcg
     1201 caagggctga ccgcgctcgg gctgctcagg gacttgaagg cgggccagtc gatcctcgtc
     1261 cacgcggcgg cgggcggcgt cggctctttg ctcgtccagc tcgccaagct cagaggcgcg
     1321 cacatcatcg gcacggcgag caccgcggag aaactcggac tcgtgaaagc gctgggcgcc
     1381 gacgcggcca tcgactacac ggcatcggac tggcccgagc aggtgaaggc ggcgacgccg
     1441 cgcggcgcgg gcgtggattt gctgatcgag atggtcggcg gcaaggtcgg cgcgcagaac
     1501 gtcaagtgtc tggcgccggg cgcgacgatg atcgtctacg gcgcggcgag cggcgaagac
     1561 ttttcgatct cggcgctcgg gctactcggc aggaacctga cggtcagggg ctacacgctc
     1621 tacgccgagt ccgagacgac gctcgccgag ttcacgcgcg agttgatgtc tctcgtcgaa
     1681 gagggtcgtt tgaaagtgac ggtggaggta ttcccgctcg cgcgggcggc ggacgcgcac
     1741 cgtgccgtcg agggccgccg cacgacgggg aaagtcgtct tgagggtttg accttcgaaa
     1801 ggatgtaggc ggatgaaaat cggaatcatc ggctctggga acatcggcgc gaacgccgcg
     1861 cggcttttcg tccgtgcggg acaccaagtc gcgctcagca attcgcgcgg cggcgaaggg
     1921 ctggaagaat tggtcgcgga actcgggccg cgtgcgcgcg cgacgacgat tgaggacgct
     1981 gcccgcttcg gcgaagtatc tctggtcgcc atccccttcg gcaacttcag agaactcccc
     2041 gccgaagcct tccgcggcaa gatcgtcctc gatgccggga actactaccc gcagcgcgac
     2101 gggctgttcg ccgaactcga caacggacag accacttcga gcgaactgct tgcctcgcac
     2161 ctcagcggcg cgcgactcgt caaaggcttc aacaccatct ggttcgagca cctcgcaaca
     2221 caaggcgaca cgaacctgcc acttcaagag cgacgcgcga tcttcatcgc cggagatgac
     2281 acgcaagcga aagagaccat cgcacgactc atcgaagaga tcggatacgc ctccttcgac
     2341 accggccccc tccgcgaagg cggcagacta caacagccgg gaacgcccat ctacaaccgc
     2401 gtgctgacgg caaaacaagc cgcggatgtt ttggcctccg cttgacacaa ggtaacgcta
     2461 tatatgaaag gtgccccgcg ggtggtagct tgaagccgct ttacgactct caactcccca
     2521 ccgcaaggag tgcctatgca gaagattctc tacgtcggga tggacgtgca tcaagcctgc
     2581 gtcgtcatcg tcgtgctcaa tgatgcgggc aagacggttt cggaagccat cattgagacc
     2641 aagaccgaga cggtcagagc tttcatccgc ggcttgcgtg gcgaagtccg cgtcacgttc
     2701 gaagaaggcg cgcaggccgc ctggctctac gagt
//
LOCUS       DBNGOH010001110         1637 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3103545, whole genome shotgun sequence.
ACCESSION   DBNGOH010001110 DBNGOH010000000
VERSION     DBNGOH010001110.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1637)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1637)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1637
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3103545"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..781)
                     /locus_tag="AC159P_21630"
     CDS             complement(<1..781)
                     /locus_tag="AC159P_21630"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008962650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0016019 - peptidoglycan immune receptor
                     activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045087 - innate immune response [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein family
                     protein"
                     /protein_id="HIR3698101.1"
                     /translation="MPFSLTWLPEVLKIAGLKVSEVPGWRDRGRAEMGRVFGVICHHT
                     VGRREGNMPSLGVLRDGRPGLPGPLAQLGLARDGTYFIIAAGRANHAGKGVWEGITNG
                     NSNFIGIEAENTGDKDDDPWPDIQIDAYHRGVAAILKRIGQSSDFCAAHREYATPIGR
                     KPDPHSLKMAAFRASVAAIMSGTAEPPKLIPGVETTTAPGAAAPRPTLRRGMTGDLTR
                     EVQRKVGAKVDGNFGPKTEAAVRAFQRDHGLVPDGIVGPKTW"
     gene            complement(781..963)
                     /locus_tag="AC159P_21635"
     CDS             complement(781..963)
                     /locus_tag="AC159P_21635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3698102.1"
                     /translation="MSEQENELNPDTAALTPEPQHAAPPDPEDEESDIDGCSEDFRKA
                     EPTPDDELPMTEGGVL"
     gene            complement(1240..>1637)
                     /locus_tag="AC159P_21640"
     CDS             complement(1240..>1637)
                     /locus_tag="AC159P_21640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3698103.1"
                     /translation="AGGYEGEGQSLRVYDAATGAHRKVMSAYYMIDKVTEVTTSTGKT
                     ALLVEMSDGGLGASYVAVVDPARGEVFFRRWAKVISRRGDNVVLGHYKETDWEKFHEN
                     ENAKVTPTKTERLNLNTVLRGRIIVNKPN"
BASE COUNT          212 a          509 c          590 g          326 t
ORIGIN      
        1 gccacgtctt cgggccgacg atgccgtcgg gcacgaggcc gtgatcgcgt tggaaggcgc
       61 gcaccgcggc ttccgtcttg gggccgaagt tgccgtccac cttggccccg actttgcgct
      121 gcacctcgcg ggtcaggtcg ccggtcatgc cgcggcgcag cgtcgggcgg ggagccgcgg
      181 cgcccggcgc ggtcgtcgtt tcgacgccgg gaatcagctt cggcggctct gccgtgccgc
      241 tcatgatggc ggcgaccgag gcgcggaagg cggccatttt gagcgagtgc gggtcgggtt
      301 tgcgaccgat gggcgtggcg tactctctgt gagcggcgca gaagtcgctg ctctggccga
      361 tgcgtttgag gatggcggcc acgccgcgat ggtacgcgtc gatctggatg tccggccacg
      421 ggtcgtcgtc tttgtctccg gtgttctcgg cctcgatgcc gatgaagttg gagttgccgt
      481 tggtgatgcc ttcccagacc cctttgccgg cgtggttggc gcgaccggcg gcgatgatga
      541 agtacgtccc gtcgcgcgcg aggccgagtt gtgcgagcgg gccgggcagg ccggggcgcc
      601 cgtcccgaag gacgccgagg ctcggcatgt tgccctccct acgcccgacg gtatggtggc
      661 agatgacgcc gaagacacgg cccatttcgg cgcgaccgcg gtcgcgccag cccgggacct
      721 ccgagacttt gaggccggcg attttgagca cttccggcaa ccatgttaag gaaaacggca
      781 ttacagtaca cctccttcgg tcatgggtaa ctcgtcatcc ggcgtcggct ccgcttttct
      841 gaagtcctcc gagcacccat caatgtcact ctcttcgtct tcggggtcgg gaggggccgc
      901 gtgctgcggc tcgggcgtga gcgcggcggt gtcaggattc agctcgttct cttgctcact
      961 catgtgtgtg ttcctctctt cgttctgatg aagtcgcaca gggttccgaa acgcttgcag
     1021 cgaggaaggc gggcgggcgt tgcgcggcga gtcgggccgg gccgggccgg tgccgacgca
     1081 ggacggagcg actcgcaggc cgcccgctcc gatccactgt cctctatttt gaatgacgtt
     1141 gtgtggagaa acgtttcgcg gcccgtcggc gggctgcggg cgagggaagt gtcgcgcggt
     1201 ggggaaggcg gacgcgggct ggttccgcgc cggtgcggtc tagttcggct tgttgacgat
     1261 gatgcgcccg cggaggacgg tgttgaggtt gagccgttcg gtcttggtcg gggtgacttt
     1321 ggcgttctcg ttctcgtgga acttctccca gtcggtctcc ttgtaatggc cgagcacgac
     1381 gttgtcgccg cgccgcgaga tgaccttcgc ccagcggcgg aagaagacct cgccgcgcgc
     1441 gggatcgacg acggcgacgt acgaagcgcc gagtccgccg tccgacatct ccacgagcag
     1501 ggcggttttg ccggtgctcg tcgtcacctc cgtgactttg tcgatcatgt agtaggccga
     1561 catgaccttg cggtgcgcgc ccgtcgcagc gtcgtagacg cggagcgact gcccctcccc
     1621 ttcgtagccg cccgccc
//
LOCUS       DBNGOH010001111         7366 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3468618, whole genome shotgun sequence.
ACCESSION   DBNGOH010001111 DBNGOH010000000
VERSION     DBNGOH010001111.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7366)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7366)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7366
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3468618"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="AC159P_21645"
     CDS             <1..270
                     /locus_tag="AC159P_21645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3698104.1"
                     /translation="LVEIEVLGQRVAVPERNRLLRCFQYLSLNTISYGDFCWNGDCTH
                     CQFWYHEEGETEAQDKTALSCRFDVRAGLVVTRLSPHVEIEGINK"
     gene            290..601
                     /gene="xseB"
                     /locus_tag="AC159P_21650"
     CDS             290..601
                     /gene="xseB"
                     /locus_tag="AC159P_21650"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:NF002140.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HIR3698105.1"
                     /translation="MAKREQTKGGAPPPANFEAQLASLERIVRELERGDLPLERSLEL
                     FEQGVRLSRDCQERLNEAERRIELLLRDGEGRTSAVAFERDEFAVEVDAEEAEEDESV
                     F"
     gene            585..1523
                     /locus_tag="AC159P_21655"
     CDS             585..1523
                     /locus_tag="AC159P_21655"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007292569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HIR3698106.1"
                     /translation="MRASSDLTRAAEATGEEVRAYAARVRALVEAELERLAPSVETGD
                     PASTVHAALRWSLFAPAKRFRPFLLFAAGETFGARADHLLRAACAYELVHTYSLVHDD
                     LPAMDDDDLRRGRPTCHVKFGEATAILAGDALQALAFQSIAEDDALDARLRVRLVAEL
                     ARAAGTPEGMVAGQAHDLAAESRRDVDGHELELIHRRKTGALIQSAARAGALVAGASA
                     AELERVARYAHDLGLLFQITDDLLDVTASAEDLGKTPGKDARSLKATYPALYGLEAAR
                     ERARLVHEAACAALDPLERDTSLLRGIARLILERRA"
     gene            1660..2679
                     /locus_tag="AC159P_21660"
     CDS             1660..2679
                     /locus_tag="AC159P_21660"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004999690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HIR3698107.1"
                     /translation="MLVLTLLPFIRSDAWWVRIFDFPRAQIAVIAAAVAAAYPFFVGA
                     YGPARYAFLAALASCVVYQSYRMFPYTPLARRQVQQSRRPREGSSLALLFANVLMENR
                     DASRLCEIIREADPDILLAIETDEWWQGQLMEFEESHPFTVLQPQGNTYGMLFYSRLE
                     LVSAEVKFLVQDDIPSIHARVRLASGQEVELHCLHPRPPFPTEDERSTERDAELLIVG
                     KAIKGKDDPVVVLGDLNDVAWSRTNDLFQHISGLLDPRKGRGFYNTFHAKYPFIRFPL
                     DHFFHSRHFRVADFRRLTYFGSDHFPVYIQLSYEPDAEAEQEKPRADESEKEEARETI
                     GKAVG"
     gene            2696..2956
                     /locus_tag="AC159P_21665"
     CDS             2696..2956
                     /locus_tag="AC159P_21665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3698108.1"
                     /translation="MSELIQRYVTEVVDDGGTTYSIFAYGEGLPGGTWEGWLEFHPSD
                     ASQPILRTGRETTQPDRRALEYWASGLEPLYFDGAFERARGG"
     gene            3031..3333
                     /locus_tag="AC159P_21670"
     CDS             3031..3333
                     /locus_tag="AC159P_21670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3698109.1"
                     /translation="MEKRKLARIEEYIFSYFRARGTSRLLAREVFESTGEYANADLVR
                     AFEDLEKKWRLLVRYTKEGNDWVTLTAEGAALAGLAPLSDEEARDVFPHPPKSSTR"
     gene            complement(3386..3676)
                     /locus_tag="AC159P_21675"
     CDS             complement(3386..3676)
                     /locus_tag="AC159P_21675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3698110.1"
                     /translation="MPESARREDSAEVPEAGVILVAFEKLFGGGRGVKRFARSGVRYV
                     ELEAGAILVEQNPKKRSEWGEAARAGRRVAWVMRDGVYLARVVEGEVVMLGG"
     gene            complement(3669..4085)
                     /locus_tag="AC159P_21680"
     CDS             complement(3669..4085)
                     /locus_tag="AC159P_21680"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3698111.1"
                     /translation="MPARILIADDYDDNRELLRLMLAADGYTIHEARNGREALDVARR
                     EVPDLALIDLSMPTLDGWGFLKELREDAGLNAIPCVAVTAFAADQDRQRALAAGFDAY
                     LSKPYRARDLLDLVRSLLPARDGDAEGRPPVTSVNA"
     gene            4642..6105
                     /locus_tag="AC159P_21685"
     CDS             4642..6105
                     /locus_tag="AC159P_21685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HIR3698112.1"
                     /translation="MRDDIENYVQSRLGGLQEEVARLQSQLNEAFTRLQERVEAAGQS
                     DPSIAVAIAEHLRYARNEGVETAAEGSARARASSDVAIIKAAVDDLGSQRTQADILDA
                     LVNRAASFAPRVAFFVVKNERATGWRARGLEGTVGDDSVREISLPLAADTLLGKVAQA
                     RSTWSGTPGTHAEDNSIYGRFGDEPPQRIVAVPLVARDKSVAVLYADSAGLDADAVNL
                     EAIEALVRVAGIAVELLAAKRPAAAEAKPSASAEHAAADAAPPPAAVTQPRPEEPQPE
                     PDPRFAREEAPPAPVSVEPEAFGSEDAGPQAAAPAEALHDDAAPPPTGIEPHVAADDA
                     DSPSPTGVEPYTPHAESFAPAAETVAPPASSTDATPLASSSSARRYGTMDMDFPVEVT
                     DEDKRFHNEARRFARLLVSEIKLYNEQKVRDGRDAGDLYDRLREDIDRSRQMYDKRVR
                     PEVAGRYDYFHHELVSALAEGDPAKLGEGYPGASVNV"
     gene            6336..7337
                     /locus_tag="AC159P_21690"
     CDS             6336..7337
                     /locus_tag="AC159P_21690"
                     /inference="COORDINATES: protein motif:HMM:NF024572.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3698113.1"
                     /translation="MSQSFHSRRTTFAVFACAALLAATLLPLTNASCSRTQQPRPDEP
                     QAFERLRALTRGGQLPAESIVAEIAAQHAGARAGALARLLQARLRLEAKDFAGAASLL
                     DAKDFARHTAVGDQALWLRAEALEQLGRRVEARAAFEQLVRDFPDSLRARAATLRASQ
                     LALKDGQPAAVPTFLEKLHDADDAEALLLTAKAYEQSGDSPRALAAYRRLYFRAPSSA
                     TNDAEAVAAFGRLGSSTAPANAAEATTRAAALAAAKRHAEAANAYTDAFARFPDTATP
                     EARVRAGVAAHNARRPDTAGILAAVPTGSDSRPEALHTIAQHHARLRQWDIVRTTLD"
BASE COUNT         1198 a         2713 c         2369 g         1086 t
ORIGIN      
        1 ctcgtcgaga tcgaggtgct cggccagcgc gtggcggtgc cggagcgcaa ccgcctgctg
       61 cgctgcttcc agtatctttc gctcaacacg atctcctacg gcgacttctg ctggaacggc
      121 gactgcaccc actgccagtt ctggtatcac gaagagggcg agaccgaggc gcaggacaag
      181 acggccctct cctgccgctt cgacgtgcgc gcggggctgg tcgtcacgcg cctcagcccg
      241 cacgtcgaga tcgaaggcat caacaagtga cggcggcttg attcatagca tggcgaaacg
      301 ggaacagacg aaaggcggcg cgccgccgcc cgcgaacttc gaggcgcagc tcgcctcgct
      361 cgaacggatc gtgcgcgagc ttgagcgcgg cgacctgccg ctcgaacgca gccttgagct
      421 gttcgagcag ggcgtacggc tgtcgcgcga ttgccaggag cggctgaacg aggccgagcg
      481 gcgcatcgag ttgctgctgc gcgacggcga gggccgcacc tcggcggtcg ccttcgagcg
      541 cgacgagttc gccgtcgagg tggacgccga agaggcggag gaagatgaga gcgtcttctg
      601 acctcacgcg cgccgcggaa gcgaccggcg aagaggtgcg cgcctacgcc gcgcgcgtgc
      661 gcgcgctcgt cgaggccgaa ctcgaaaggc tcgccccgtc ggtcgagacg ggcgaccccg
      721 cgtccacggt ccacgccgcg ctgcgctgga gcctcttcgc gcccgccaag cgcttccgcc
      781 ccttcctcct cttcgccgcc ggcgagacct tcggcgcgcg cgccgaccat ctgttgcgcg
      841 cggcctgcgc ctacgagctt gtccacacct actcgctcgt ccacgacgac ctgcccgcga
      901 tggacgacga cgacctccgg cgcgggcgcc cgacctgcca cgtcaagttc ggcgaggcca
      961 cggccatcct cgccggcgac gccctgcaag ccctcgcctt ccaatccatc gccgaggacg
     1021 acgcgctcga cgcgcgtttg cgcgtccgcc tcgtcgcgga gctggcgcgc gccgccggaa
     1081 cgcccgaagg catggtcgcg gggcaggccc acgacctcgc cgccgaatcg cgccgagacg
     1141 ttgacggcca cgaactcgaa ctcatccacc gccgcaagac cggcgcgctc atccagtccg
     1201 ccgcgcgcgc gggcgcgctc gtcgcgggcg cctcggcggc ggaactcgaa cgggtcgcgc
     1261 gctacgccca cgacctcggc ctcctcttcc agatcacgga cgacctgctg gacgtgacgg
     1321 cctcggcgga agacctcggc aagacgcccg gcaaggacgc gcgctcgctg aaggccacct
     1381 accccgcgct ctacggcctc gaagccgcgc gcgagcgcgc acgactcgtc cacgaagcgg
     1441 cctgcgccgc gctcgacccg ctggagcgcg acacctcgct gctgcgcggc atcgcccgcc
     1501 tcatcctcga acgccgcgca tgaaggcgcc gcgctccggc cccggcatta gaacaccccc
     1561 cggatttgag ctagaattcc aagactcgtg cgccacactt ttaatcgccg gtcaggggac
     1621 ttttaggtgg agtggtttct aaccgcagtc gggggcttga tgttggtctt gaccttgctc
     1681 cctttcatca ggtcggacgc gtggtgggtg aggattttcg acttcccgcg cgcgcagatc
     1741 gcggtcatcg cggcggcggt cgcggcggcc tacccgttct tcgtcggcgc ttacggcccg
     1801 gcgcgctacg ccttcctcgc cgcgctcgcc tcttgcgtcg tctaccagtc gtacaggatg
     1861 ttcccctaca cgccgctcgc gcgcaggcag gtgcagcaga gccgccgccc gcgcgaaggt
     1921 tcgagcctcg cgctgctgtt cgccaacgtg ctgatggaga accgcgacgc gtcgcgcctt
     1981 tgcgagatca tccgcgaggc agacccggac atcttgctcg ccatcgagac ggacgagtgg
     2041 tggcaggggc aactcatgga gttcgaagag tcgcacccct tcacagtcct tcagccgcag
     2101 ggcaacacct acgggatgct cttctattcg cggctcgaac tcgtgagcgc ggaggtgaag
     2161 ttcctcgtgc aggacgacat cccttccatc cacgcgcgcg tccgattggc ctcgggtcag
     2221 gaggtcgaac tccactgcct ccacccgcgc ccgcccttcc ccacagagga cgagcgctcg
     2281 accgagcgcg acgccgagct gctcatcgtc ggcaaggcca tcaagggcaa ggacgacccc
     2341 gtcgtcgtcc tcggcgacct caacgacgtg gcgtggtcgc ggacgaacga cttgttccag
     2401 cacatcagcg gactcttaga cccgcgcaag ggccgcggct tctacaacac cttccacgcc
     2461 aagtacccct tcatccgctt ccccctcgac cacttcttcc actcgcgcca cttccgcgtc
     2521 gcggacttcc ggcgcctcac ctacttcggc tccgaccact tccccgtcta catccaactc
     2581 agctacgagc cggacgccga ggccgagcag gagaagccgc gcgccgacga gtccgaaaaa
     2641 gaagaggcgc gtgagacaat cggcaaggcg gtcggttaaa aggagggttc gcacgatgtc
     2701 cgagttgata cagcgttatg tgaccgaggt cgtggacgac ggcggcacga cttacagcat
     2761 cttcgcgtac ggcgaggggc tgccgggcgg gacgtgggaa gggtggctcg aattccaccc
     2821 gtcggacgcg tcgcagccaa tcttgcgcac ggggcgcgag acgacgcagc ccgaccgccg
     2881 cgcgctcgaa tactgggcct ccggcttgga gccgctctac ttcgacggcg ccttcgagcg
     2941 agcgcgcgga ggctgacggc ggtgcggtcg cccgaccgtc tcttatcatt tccgagttga
     3001 cgcgacgaga ttcgaaggga cgtgacgccg atggagaagc ggaagctggc gcgcatcgag
     3061 gagtatatct tctcctactt tcgcgcgcgc gggacgagca ggctgcttgc gcgggaggtg
     3121 ttcgagagca ccggcgagta cgcgaacgca gacctcgtcc gcgccttcga agatttggag
     3181 aagaagtggc ggctgctcgt ccgctacacg aaggagggca acgactgggt cacgctcacg
     3241 gcggagggcg ccgcgctcgc gggcctcgcg ccgctctcgg acgaagaggc ccgcgatgtc
     3301 ttcccgcacc cgcccaagag ttcgacgcgc tgacgcctgc gacggacgga ggagcacgac
     3361 gcctcccgtc atccgtcgtc ccggttcatc cgccgagcat gacgacttcg ccttcaacga
     3421 cgcgcgcgag atacacgccg tcgcgcatca cccatgcgac gcggcggccc gcgcgcgccg
     3481 cctcgcccca ctcgctccgc ttcttcgggt tctgttcgac gaggatcgcg cccgcttcaa
     3541 gctccacgta gcgcacgccc gagcgcgcga agcgcttcac gccgcgaccg ccgccgaaga
     3601 gcttttcgaa cgcgacgagg atgacgcccg cttcgggcac ctcggcggag tcctcgcgtc
     3661 gtgcgctctc aggcattgac cgaggttacc ggcgggcggc cttcggcgtc gccgtcgcgt
     3721 gcggggagca ggctgcggac gaggtcgagg aggtctctgg cgcggtaggg tttcgagagg
     3781 taggcgtcga agcccgcggc gagcgcgcgc tgtcgatcct ggtcggcggc gaaggccgtg
     3841 acggcgacgc acgggatggc gttcaggccc gcgtcctcgc gcagctcttt gaggaagccc
     3901 cagccgtcga gcgtcggcat cgagaggtcg atcagcgcga ggtcgggcac ctcgcgccgc
     3961 gccacgtcca gcgcctcgcg cccgttgcgc gcttcgtgga tggtgtagcc gtcggcggcc
     4021 agcatcaggc gcagcagttc ccggttgtcg tcgtagtcgt cggcgatgag tatgcgagca
     4081 ggcatcttcg tcgggtgtgt ctttcggcaa agcgggggga gcgcggcgcg tttcgcgtct
     4141 cggcaagagc cttcgcctct cgccgacggc acaaggcaga acgtcgcccg ctcggacatc
     4201 ggggggggaa ccgtcgcgcg ccgttatagc gcgagcggtc gggcagagtc aacccggaag
     4261 gcaggaggcc aaaccgaagg caaaaggcag agggcaaagg gcaaaaggca aaaaggtgga
     4321 cgaagctgtg cttcgatttt cgatttctta tctcgcgccg cagacgcgcc cgcccttttg
     4381 ccttttgcct cttgcctttt gccttccgcg acagcggttt gccttttgcc ttgacgagtc
     4441 tccgcccgcg tgtccataat tctcgcggct ggttcttccg cgcggagggt cttgtcaaca
     4501 gccgcaccgt catacaaaca agagcgccgc cggagattcc cccctgtctc gaccggcccg
     4561 ttagaatgct ccagcctcga agcactttca cgcctaaacg gaggaaacga aaaggtgtcc
     4621 agcggagact tggaaaagag cttgcgcgac gacattgaga actacgtcca gagccgcctc
     4681 ggcggcttgc aggaagaggt ggcgcggctt cagagccagt tgaacgaagc cttcacgcgc
     4741 ctgcaagagc gcgtcgaggc ggccggccag tccgaccctt ccatcgccgt cgccatcgcc
     4801 gagcacctgc gctacgcgcg caacgagggg gtcgagaccg ccgccgaagg ctcggcgcgc
     4861 gcccgcgcct cttccgacgt ggcgatcatc aaggccgccg tggatgacct cggctcgcag
     4921 cgcacgcagg ccgacattct cgacgcgctc gtcaaccgcg ccgcctcctt cgccccgcgc
     4981 gtcgccttct tcgtcgtcaa gaacgagcgc gccaccgggt ggagggcgcg cggactggaa
     5041 ggcactgtgg gcgacgactc ggtgcgcgaa atctcgctgc cgctcgcggc ggacacgctg
     5101 ctcggcaagg tcgcgcaggc gcgctcgacg tggagcggca cgcccggcac gcacgccgag
     5161 gacaactcga tctacggtcg cttcggcgac gagccgccgc agcgcatcgt cgccgtcccg
     5221 ctcgtcgcgc gcgacaagtc ggtcgccgtg ctctacgccg acagcgccgg cctcgacgcc
     5281 gacgccgtca acctcgaagc catcgaagcg ctcgtgcgcg tcgccggcat agccgtcgaa
     5341 ctgctcgccg ccaagcgtcc tgccgcggcg gaggcgaagc cctccgcctc cgccgagcac
     5401 gcggcggcgg acgccgcgcc gccgcccgcc gccgtgacgc agccgcggcc cgaagagccg
     5461 cagcccgaac cggacccgcg gttcgcgcgg gaggaagcgc cgcccgcgcc cgtctccgtc
     5521 gagccggaag cgttcggctc cgaagacgcc ggcccgcagg ccgccgcgcc cgccgaagcc
     5581 ctgcacgacg acgccgcgcc gccgcccacg ggcatcgagc cgcacgtcgc cgccgacgac
     5641 gccgactccc caagcccgac cggcgtcgag ccgtacacgc cgcacgccga atcgttcgcg
     5701 cccgccgccg aaacggtcgc gccgcccgcc tcctcgactg atgccacgcc gctcgcctcg
     5761 tcttcctccg cgcgccgcta cggcacgatg gacatggact tccccgtcga agtcaccgac
     5821 gaggacaagc gcttccacaa cgaggcgcgc cgcttcgccc gcctgctcgt ctccgagatc
     5881 aaactctaca acgagcagaa ggtgcgcgac gggcgcgacg ccggagacct ctacgaccgc
     5941 ctgcgcgaag acatcgaccg ctcgcgccag atgtacgaca agcgcgtgcg gcccgaggtg
     6001 gccgggcgct acgactactt ccaccacgag ctggtctccg cgctcgccga gggcgacccc
     6061 gccaagctcg gcgaaggcta ccccggcgcg agcgtcaacg tctgacagct tacagtcgga
     6121 acgccgaagg gtgaacggaa agtcccgcga ctctctgttc accctttcgc ctttttgttt
     6181 tatccttcac aggcgaaccg ccatcggaaa cgatgaatga tgaatgcgga atgatgaatg
     6241 atgaataaaa gaagggttga atgatgacag gcaccgtgct tctcattgat cattcatcat
     6301 tccgcattca tctttacaga aaggtacacg acgctttgag ccagagtttc cacagccgcc
     6361 gcaccacctt cgccgtcttc gcctgcgccg cgctgctcgc ggccaccctg ctgccgctca
     6421 cgaacgccag ttgcagccgg acgcagcagc cgcgccccga cgagccgcaa gccttcgagc
     6481 gcctgcgcgc gctgacgcgc ggcgggcaac tcccggcgga gtcgatcgtc gccgagatcg
     6541 ccgcgcagca cgcgggcgcg cgcgcgggcg cgctcgcgcg tctcttgcag gcgcggctca
     6601 ggctcgaagc gaaagacttc gcgggcgcgg cctcgctgct cgacgcgaag gacttcgcgc
     6661 gccacaccgc cgtcggcgac caagccctct ggctgcgcgc agaggctctg gagcaactgg
     6721 gccgccgcgt cgaggcgcgc gccgccttcg agcagctcgt gcgcgacttc cccgactcgc
     6781 tccgcgcgcg cgcggccacg ctgcgcgcct cgcagctcgc gctcaaggac ggccagcccg
     6841 ccgccgtccc caccttcctc gaaaaactcc acgacgccga cgacgccgaa gcgctcctgc
     6901 tcacggccaa agcctacgag cagagcggcg actctccgcg cgcgctcgcc gcctaccgcc
     6961 gcctctactt ccgcgcgccg tcatcggcga cgaacgacgc cgaagcggtc gccgccttcg
     7021 gtcggttagg ctcttcgacc gcgcccgcca acgccgcgga ggcgacgacg cgcgccgccg
     7081 cactcgccgc cgccaagcgc cacgccgaag ccgccaacgc ctacacggac gccttcgcgc
     7141 gcttccccga caccgcgacg cccgaggcgc gcgtccgcgc cggggtcgcc gcgcacaacg
     7201 cgcgccgccc cgacaccgcc ggcatcctcg ccgccgtccc caccggctcc gactcgcgcc
     7261 ccgaagcgct gcacaccatc gcgcaacacc acgcgcgcct gaggcagtgg gacatcgttc
     7321 gcaccacgct cgactagatg cgccgcgcct tccctaacaa tgaatg
//
LOCUS       DBNGOH010001112         4854 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2184805, whole genome shotgun sequence.
ACCESSION   DBNGOH010001112 DBNGOH010000000
VERSION     DBNGOH010001112.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4854)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4854)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4854
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2184805"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(36..119)
                     /locus_tag="AC159P_21695"
     tRNA            complement(36..119)
                     /locus_tag="AC159P_21695"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(83..85),aa:Leu,seq:cag)
     gene            complement(288..845)
                     /locus_tag="AC159P_21700"
     CDS             complement(288..845)
                     /locus_tag="AC159P_21700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3698114.1"
                     /translation="MRGRKFSLKAVTFAFALLMALPTFALAAQKNKNRNRKQDVFVNG
                     HDARDGRWDGRGPRRDDSRYDDNQRRRRRDRDYNDDDYNRRRNRRNDDYSRQRRRDGR
                     YDDGYGYGSSSQLRQTALNAGYNEGIKEGRNDRRRGDRYDYRDESDYRNATRDYNSRY
                     GDRGQYQQYFRQGFANGYRDGYNGY"
     gene            1158..1886
                     /locus_tag="AC159P_21705"
     CDS             1158..1886
                     /locus_tag="AC159P_21705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HIR3698115.1"
                     /translation="MQRAEHEPETLRARLERVRARVEASARRAGRAPDEVELIAVSKT
                     QPAAVLREALAAGVRDFGENRVQEAVEKIEELEAGEGVRWHLIGHLQSNKARRAVKLF
                     DCIQSVDSAALVERLERVCAEEGRESLDVLLQLDLAGEATKTGAGVEELPALVAALGE
                     CERVRCRGLMTLPPYFEDAERVRPFFRRLRELRDELGARGLFGGAAGQLSMGMSHDFE
                     VAVEEGATMVRVGTAIFGARGAAH"
     gene            1917..2600
                     /locus_tag="AC159P_21710"
     CDS             1917..2600
                     /locus_tag="AC159P_21710"
                     /inference="COORDINATES: protein motif:HMM:NF014389.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HIR3698116.1"
                     /translation="MNIIAFFQTFRVVGQGPPNRAVQLLDSAFQVTWRVIVAIIVAMI
                     VLMLVRFLLNYADLNPFSRPVIIVRRMTDQFVNPVRRALAGFGVNPNGAPLVVILLTI
                     LLGYFVMMLTASLLNTAAGVAVSVSSGQAGGVVALLGYVLYGLLSLYSLLIVIRIVFS
                     WGQVGYGNRVMRFLINATDPVLVPLRRMIPPLGMFDISPIVAFIIIWLFQAAIRMTLL
                     RGWPTEFFM"
     gene            2615..2905
                     /locus_tag="AC159P_21715"
     CDS             2615..2905
                     /locus_tag="AC159P_21715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HIR3698117.1"
                     /translation="MVECSESGGAVSFAVRVAPRAARTEIAGEHGGALKVRVAAPPVE
                     GAANEELVRFLARHFGVPARDVEIVAGHSSKTKRVRLRGITSEQLARLASSG"
     gene            complement(3031..3477)
                     /locus_tag="AC159P_21720"
     CDS             complement(3031..3477)
                     /locus_tag="AC159P_21720"
                     /inference="COORDINATES: protein motif:HMM:NF024865.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4440 domain-containing protein"
                     /protein_id="HIR3698118.1"
                     /translation="MKRILLLIMVVAAATTTVFGQTGGDEKALNQFLDGYLAALPKNS
                     ADVVAPFLGGQYMRVGPDGSSINKEQMLAALRSGDLKYASVAADERTWRMFGRDVAIS
                     TSRVTLKASHKGQDMSGTYRATTVLWKNGSAPWVLVSTHLSRLEAK"
     gene            complement(3637..4092)
                     /locus_tag="AC159P_21725"
     CDS             complement(3637..4092)
                     /locus_tag="AC159P_21725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3698119.1"
                     /translation="MRTKRFLAAALAASLLLLAAGVYAQVSRPYRNGSVWDVAFIRMK
                     PGMESAYLDYIATDWKRNQEELKKAGLIVSYKVLTTEPHNPGDFNILLMTEYKDLASL
                     EASEDKADALMQRVIGNDDKQRQGYKERLEIREVMGNRLAREIVLEPRR"
BASE COUNT          771 a         1544 c         1632 g          907 t
ORIGIN      
        1 actattcacc atttaccatt taccgctttt cctgatgcgg atgagtggaa tcgaaccacc
       61 acggtgtcac caccacaggc ttctgagacc tgcgcgtcta ccagttccgc cacatccgct
      121 cgtgaacggt gaaaatcaat atacggaggc gcgcgcgcgg gtgtcaagcg gggccgccgg
      181 ggcgcgacaa cggggcttcg gcagcaagac cgaagccccg cgcccgatgc ggcggaggcg
      241 cccgccgcgt gcatgccgga gggctgaccg ggggccgccc ctccgtttta gtaaccgttg
      301 tagccgtcgc ggtagccgtt ggcgaagccc tgacggaagt actgctggta ctgaccgcgg
      361 tcgccgtagc gcgagttgta gtccctcgtc gcgttgcggt agtcgctctc gtcgcggtag
      421 tcgtagcggt cgccgcggcg acggtcgttg cgcccttcct tgataccttc gttgtagccg
      481 gcgttgagcg ccgtctgccg gagctgggac gagctgccgt agccgtagcc gtcgtcgtag
      541 cgcccgtcgc ggcggcgttg acggctgtag tcgtcgttgc ggcggttgcg acggcggttg
      601 tagtcgtcgt cgttgtaatc gcggtcgcgg cggcggcgac gctggttgtc gtcgtagcgg
      661 ctgtcgtcac gcctcgggcc gcgcccgtcc cagcgcccgt cacgcgcgtc gtggccgttg
      721 acgaaaacgt cctgcttgcg attcctgttc ttgttcttct gcgcggccag cgcgaacgtc
      781 ggcagcgcca tcagcagggc gaacgcgaac gtgacggcct tcagggaaaa tttccgacct
      841 ctcatcttct ttgtatcctc ctcgaatttc gctggtgcga ttgcaacacg cggcgggtaa
      901 tctttccggc ctcttcacga aatcaatcac cgtgccgcgc gcgccgccgc catcaaaacg
      961 tcaagattac gggccggacg cgccggacgg gcgaccgccg ggtgcaccat aaagtgtcac
     1021 gccgtagcgt atcgtatggc gtcgggtggc gcgcggggcg catggggcgc gcgttcgtgc
     1081 cgcttgggga gtcctgttgc gggcgtgctg gcgcgcttgt tatagtctcc cttcgattgc
     1141 aaccaaagcg catccgcatg cagagagcag agcacgagcc ggagaccttg cgcgcgcgac
     1201 tcgaacgtgt gcgcgcccgc gtcgaggcga gtgcgcgcag ggccggtcgc gcgccggatg
     1261 aggtggagct gatcgccgtc agcaagacgc agccggcggc ggtgctgcgc gaggcgctgg
     1321 cggcgggcgt gcgcgacttc ggcgagaacc gcgtgcagga ggccgtggag aagatcgagg
     1381 agctggaggc gggcgagggc gtccgctggc acctcatcgg ccacctgcaa tcgaacaagg
     1441 cgcggcgcgc ggtcaagctc ttcgactgca tccagtcggt cgattcggcg gcgctcgtcg
     1501 agcggctcga acgcgtctgc gccgaagagg gaagggagag cctcgacgtg ctgctacagc
     1561 tcgacctcgc gggcgaggcg acgaagacgg gcgcgggcgt cgaagagctg cccgcgctgg
     1621 tcgccgcgct gggggagtgc gagcgcgtcc gctgccgcgg gctgatgacg ctgccgccct
     1681 acttcgaaga cgccgagcgc gtccgcccct tcttccgccg cctgcgcgag ctgcgcgacg
     1741 agctgggtgc gcgcggcctc ttcggcggcg cggcgggcca gctctcgatg ggcatgagcc
     1801 acgacttcga ggtcgccgtc gaggagggcg cgacgatggt gcgcgtcggc acggccatct
     1861 tcggcgcgcg gggggcggca cactagcccg gcactccggg cgccatacga atcaccatga
     1921 acatcatcgc ctttttccag acatttcggg tggtcgggca ggggcctccg aaccgcgccg
     1981 tgcagcttct cgacagcgcc ttccaggtca cctggcgagt gatcgtggcg atcatcgtcg
     2041 ccatgatcgt gctgatgctg gtgcgcttcc tcctcaacta cgccgacctc aacccgttca
     2101 gccgtcccgt catcatcgtg cggcggatga ccgaccagtt cgtcaacccg gtcaggcgcg
     2161 cgctcgcggg gttcggcgtc aacccgaacg gagcgccgct cgtcgtcatc ctcttgacga
     2221 tcctgctcgg ctacttcgtg atgatgctca cggccagcct gttgaacacg gcggcgggcg
     2281 tcgcggtgag cgtgtcgagc gggcaggcgg gcggcgtcgt ggcgcttctc gggtatgtgc
     2341 tctacgggct gctctcgctc tactcgctgc tcatcgtcat ccgcatcgtc ttctcgtggg
     2401 ggcaggtagg ctacggcaac cgcgtgatgc gtttcctcat caacgcgacc gaccccgtcc
     2461 tcgtcccgct gcggcggatg atcccgccgc tcggcatgtt cgacatctcg cccatcgtcg
     2521 ccttcatcat catctggctc ttccaggcgg ccatcaggat gaccctcctg cgcggctggc
     2581 cgaccgaatt cttcatgtga ggcgcggggc gcggatggtc gaatgctcgg agtcgggcgg
     2641 cgccgtcagc ttcgccgtgc gcgtcgcgcc gcgcgccgcg cgcaccgaga tcgcgggcga
     2701 gcacggcggc gcgctcaaag tccgcgtcgc cgcgccgccc gtcgagggcg ccgccaacga
     2761 ggagctggtg cgcttcctcg cgcgccactt cggcgtgccc gcgcgcgacg tggagatcgt
     2821 cgccggccac tcctcgaaga ccaaacgcgt ccgcctccgc ggcatcacgt cggagcagct
     2881 cgcgcggctc gcctcttccg gatagaaccc cacaacaaag cgggcggtca atccgctacg
     2941 gctcacatca cgtgaaaggc cccggcgtgt tgaccggggc cttcatagag agtctggccg
     3001 ctgccgaacc tgcgccgcac tcacatccct ttacttcgct tcgaggcggc tgaggtgtgt
     3061 cgagaccagc acccacgggg ccgagccgtt cttccacagc acggtcgtgg cgcggtaggt
     3121 gccgctcatg tcctgcccct tgtggctggc cttgagcgtc acccgcgagg tcgagatggc
     3181 gacatcgcga ccgaacatcc gccacgtccg ctcgtccgcc gcgacggagg cgtatttcaa
     3241 gtcgccggag cgcagggccg cgagcatctg ttctttgttg atgcttgagc cgtcggggcc
     3301 gacccgcatg tactggccgc ccaaaaacgg cgcgaccacg tcggcggagt ttttcgggag
     3361 cgcggccaga tagccgtcga gaaactggtt caacgccttt tcgtcgccgc ccgtctggcc
     3421 gaatacagtg gtcgtggcag ccgccacgac cattatcagt agaaggattc gcttcatgct
     3481 gtctctcctc gtcggggtta agccggaagg aaccggcggg ggcgggacgc gctgctgatg
     3541 cggcgcgtcc cggatcgcgt taattgcccg gaggcgtcga gcctccgtcg tgactgcgac
     3601 tgcctttccg tctcaccgcg gggtcggggg cgggcgtcag cggcgcggct ccaggacgat
     3661 ctcccgcgcc agcctgttgc ccatcacctc ccgaatctcc aaccgctcct tgtagccctg
     3721 cctctgcttg tcgtcgttgc cgatgacccg ctgcatcaag gcgtccgcct tgtcctcgct
     3781 cgcctccaac gaggccaagt ctttgtactc ggtcatgagc aggatgttga agtcgccggg
     3841 gttgtgcggc tcggtcgtca gcaccttgta cgagacgatc agacctgcct tcttcagttc
     3901 ctcctgattc ctcttccagt cggtggcgat gtagtcaagg taggccgact ccatccccgg
     3961 cttcatcctg atgaacgcga catcccagac cgagccgttt cggtaggggc ggctgacttg
     4021 cgcgtagacg ccggcggcga gcagcagcag ggacgctgcc agcgcggcgg ccagaaatcg
     4081 tttcgttctc atggtggact cctgtcccgt acttaggggt tagcgggttc gcgggcgacg
     4141 tacgggggaa cccgcgccgc gaatccgtga gaggaaagac gagaggtttg ccggtgggaa
     4201 ggactacagc tggctagtgc gcggggactg tacgggaaaa tggagttcag gtcaacaatt
     4261 gagacgtgtc gtcgggcgga cgatcatgtc gcgcgccgag aagtcgtctc gtccggaggt
     4321 ggaggcagct tttccgcggc aatacagtag cggcaggtga gggtgtggag catgattagt
     4381 tcgcccgaca ttacatcgaa aagtgtaatg aaattatcca actggaaccg catatagcaa
     4441 tgaaaagtgg cttaacaaat cagcctttct gtgggtataa gttgctcatt ctaaatagct
     4501 tagagtctcg cggggtcttg acgggccagg agggggcgtg gtagcctctg aggtgtcgaa
     4561 aatgccccgg aaagggcgat gggcgtaacg gtggactgtc ctgagaaaac gccaccgtta
     4621 cgcccagctg aacaaacaat ctgcccgcag ccttaaaagc tgcggccgga cccgtttgcc
     4681 gtcgtcatta tattgggctg aaagaccgtg agcaaggcaa tggagaaacg aaaggcgggt
     4741 gccgacatgg tcgagggtcg ccctgtcccg gaaaaggagg cggctttcgc tctcaatctc
     4801 tctgtcagga cgttgaagag gctgcggcgc gcgggggcca ttaaacacct tcgc
//
LOCUS       DBNGOH010001113         2299 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4020040, whole genome shotgun sequence.
ACCESSION   DBNGOH010001113 DBNGOH010000000
VERSION     DBNGOH010001113.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2299)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2299)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2299
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4020040"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            437..2062
                     /locus_tag="AC159P_21730"
     CDS             437..2062
                     /locus_tag="AC159P_21730"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017721852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase"
                     /protein_id="HIR3698120.1"
                     /translation="MTAIKQAATREHGRRRSDVGLDALGIKAPANVYWNLNTPELYEE
                     IARRGEGLYSAHGAVVVDTGEHTGRAAKDKAIVREPSSEDKVFWGEVNKEFTQGAVNA
                     LRDRMMQHTAGRDLFVQDTYAGADPRYRLPVRVVNELAWHSLFARTMFINDDAGREQH
                     TPEFTVVNLPSFKADPERDGTRSPTFILMDFSQRLVLIGGTSYAGETKKSVFTILNYL
                     LPQRGVMSMHCSANVGHAGDVAVFFGLSGTGKTTLSADPERRLIGDDEHGWSDDGVFN
                     FEGGCYAKVIKLSAEAEPDIYRTTRMFGTVMENVVVDPVTRVPDLDDAAKTENTRAAY
                     PLTSIPNIVPEGYAGHPSNIIMLTADAFGVLPPVARLSPEQAMYHFLSGYTAKVAGTE
                     RGVKEPEATFSTCFGAPFMVLHPGVYADLLGKKIQEHGAACWLVNTGWSGGPYGVGSR
                     MSIKHTRAMIRAILNGTLASVETRPDPVFGVGVPVAVAGVPDDVLTPRNTWQDKEAYD
                     RKALELARRFNENFKKYEAGVSDAVRAVAPKAG"
BASE COUNT          420 a          805 c          728 g          346 t
ORIGIN      
        1 tgcacgcgcc cgagagcggc gacgacctcg acgcctgcgc cgaccgcctg gaacgcgccg
       61 cgcgcgacat ggctgtcgcc gtccgcgagc tggccgaaca gcgcggcgga agtctttcgc
      121 agtaaaagct tcaaggggcc gacagtttca tcaaacatcc tgcgggagcg cccgcgcgag
      181 ccttcaaccg aaggcccgcg gcgctcctgc cgcacgttgg ccctctgttt tgttcggtcg
      241 gccaacaaag gacgagcgag gagcgcaccc atgtccggcg acgagatgga gcgccatcga
      301 gttcgtacag aataatgcca agctcgtaga gcgacttaag cagacgcagg cgcagatcgc
      361 cgagactaac cgcatcgtgc actcgcgact cgaagacgat tcaccattga ccatcgacta
      421 ttcatcagga gaacttatga ccgccattaa acaggccgcg actcgtgagc acgggcggcg
      481 gcgcagcgac gtggggctgg acgcgctcgg catcaaggcg ccggccaacg tctactggaa
      541 tctgaacacg ccggagttgt acgaggagat cgcgcggcgc ggcgaggggc tgtactcggc
      601 gcacggcgcg gtcgtggtgg acacgggcga gcacaccggg cgcgcggcca aggacaaggc
      661 catcgtccgc gagccttcca gcgaggacaa ggtcttctgg ggcgaggtga acaaggagtt
      721 cacgcaaggt gcagtcaacg ccctgcgcga ccgcatgatg cagcacacgg cggggcgcga
      781 cctcttcgtg caggacacct acgcgggcgc tgacccgcgc taccgcctgc ccgtccgcgt
      841 cgtcaacgaa ctggcgtggc actcgctctt cgcccgcacg atgttcatca acgacgacgc
      901 gggccgtgag cagcacacgc ccgagttcac cgtggtcaac ctccccagct tcaaggccga
      961 ccccgaacgg gacggcacgc gctcgcccac cttcatcctg atggacttct cgcagcgcct
     1021 cgtcctcatc ggcggcacct cttatgcggg cgagacgaag aagtcggtct tcaccatcct
     1081 caactacctg ctcccgcagc gcggcgtgat gtcgatgcac tgctcggcga acgtcggcca
     1141 cgcgggcgac gtggcggtct tcttcgggct gtcggggacg ggcaagacga cgctctcggc
     1201 agaccccgag cgccgcctca tcggcgacga cgagcacggc tggtcggacg acggcgtctt
     1261 caacttcgag ggcggctgct acgccaaagt catcaagctc tccgccgagg ccgagccgga
     1321 catctaccgc acgacgcgca tgttcggcac cgtcatggag aacgtcgtcg tcgatccggt
     1381 gacgcgcgtg cccgacctcg acgacgccgc gaagaccgag aacacgcgcg ccgcctaccc
     1441 gctcacctcc atccccaaca tcgtccccga gggctacgcc ggccacccct cgaacatcat
     1501 catgctcacg gcggacgcct tcggcgtgct gccgccggtc gcgcgcctta gccccgagca
     1561 ggcgatgtac cacttcctct ccggctacac ggcgaaggtc gcgggcaccg agcgcggcgt
     1621 caaggaaccc gaggccacct tctcgacctg tttcggcgcg cccttcatgg tcttgcatcc
     1681 gggcgtctac gcggacttgc tcggcaagaa gattcaggag cacggcgccg cctgctggct
     1741 cgtcaacacg ggctggtcgg gcggccccta cggcgtgggg tcacgcatgt ccatcaagca
     1801 cacgcgcgcg atgatccgcg ccatcctcaa cggcacgctc gcgtccgtgg agacgcgccc
     1861 cgacccggtc ttcggcgtcg gcgtgcccgt cgcggtggcc ggcgtgcccg acgacgtgct
     1921 gacgccgcgc aacacctggc aggacaagga agcctacgac cggaaggcgc tcgaactcgc
     1981 gcgccgcttc aacgagaact tcaagaagta cgaggccggc gtctcggacg ccgtgcgcgc
     2041 cgtcgcgccg aaggctgggt gaaagaccgg ggccggtgag tttgccgcgc gctcatttta
     2101 acgcggagga cgcggagggg cgccgaggac gcagagaaaa ccagttgtct tctctgcgtc
     2161 ctcggcgccc ctccgcgtcc tccgcgttga atcctccccc cggtaaacta aaccgcacat
     2221 ggctccgggc ttcctgaagt cgtcaatcag tcatcgagaa gatacatggc gagcaccttg
     2281 tagccgctcc cgactctga
//
LOCUS       DBNGOH010001114         1740 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2003191, whole genome shotgun sequence.
ACCESSION   DBNGOH010001114 DBNGOH010000000
VERSION     DBNGOH010001114.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1740)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1740)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1740
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2003191"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..821)
                     /locus_tag="AC159P_21735"
     CDS             complement(<1..821)
                     /locus_tag="AC159P_21735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3698121.1"
                     /translation="MPETFDWLKPSPLWEAGAADMRRQDFFRPVLHEFRADTFMEDFL
                     AAAAAPGPRRFRDSLAKLLPGRTTLKMFQPVHGCFYLASASLCCRIPGFPDREVSPGA
                     GESVFFVLRKRVNGVEYGWDVGAAGNAWKPLGTKLVLDAEERLPLLQTPSAAGRNVFF
                     GYVPVASRETYATAPTAPNSPIDFTKDLRLEELHARFIEPLAPPPPIDAPAFGPTNAR
                     ATGAALIRRITVYLLLDLWEFFETYLPNVANAIRDNTTSTLVGPPRALTDYLEQQR"
     gene            complement(937..1647)
                     /locus_tag="AC159P_21740"
     CDS             complement(937..1647)
                     /locus_tag="AC159P_21740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007705104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HIR3698122.1"
                     /translation="MTGIVLEVLEQCDAAHDRMRLYGAYPATVTDIRDPDSQGRVKVK
                     LPWMPDNDGSGYDVWARLATMMAGNNRGTWFIPDVGDEVLVVFEGGDARRPYVVGSLW
                     NGSDAPPEQMDGAGNNYKKSIVSRRDIRITLDDTEGAETLTLKTPQNQSIEIKDGGLS
                     IEIKDASGNSIKLEPAGVTITTSAQVKVQASTVEVSAGMVTVNAGMSKFSGVVQCDTL
                     ISNAVISASYTPGAGNIW"
BASE COUNT          260 a          552 c          635 g          293 t
ORIGIN      
        1 cgttgctgtt cgaggtagtc ggtgagcgcg cgcggcgggc cgacgagcgt cgaggtcgtg
       61 ttgtcgcgga tggcgttggc cacgttcggc aggtaggtct cgaagaactc ccagaggtcg
      121 agcagcaggt agacggtgat acggcggatg agcgccgcgc cggtggcgcg ggcattggtc
      181 ggcccgaagg cgggcgcgtc aatcggcggc ggcggggcga gcggctcgat gaagcgcgcg
      241 tgcagctctt cgagccgcag gtctttggtg aagtcgatgg gcgagttcgg cgcggtcggg
      301 gccgtcgcgt aggtctcgcg gctggcgacg gggacgtagc cgaagaagac gttgcgcccc
      361 gcggcggacg gcgtctgcaa gagcggcagg cgctcctccg cgtcgagcac gagcttcgtg
      421 ccgagcggct tccaggcgtt gcccgccgcg cccacgtccc acccgtactc gacgccgttc
      481 acgcgcttgc gcagcacgaa gaagacgctc tcgcccgcgc ccggcgagac ctcgcggtcg
      541 gggaagccgg ggatgcggca gcagagcgag gccgacgcga ggtagaagca gccgtgcacg
      601 ggctggaaca tcttcagcgt cgtgcgcccc ggcaggagct tcgccagcga gtcgcggaag
      661 cgccgcggcc cgggcgcggc ggcggcggcg aggaagtcct ccatgaaggt gtcggcgcgg
      721 aactcgtgca gcaccgggcg gaagaagtcc tgccgccgca tgtctgccgc gcccgcctcc
      781 cacagcggcg aaggctttaa ccagtcgaac gtctcgggca taagcttaaa cgattgcgga
      841 ttgcggattg cggagtgttc ggtcgccgcg ggtcgaaagt cttacatcaa gccgcaagct
      901 tgggcggcac gccaatccgc aatccgcaat ccaaaatcac cagatgttcc ccgcgccggg
      961 cgtgtagctg gcgctgatga cggcgttgct gatgagcgtg tcgcactgga cgacgccgct
     1021 gaacttggac atgccggcgt tgaccgtgac catgccggcg ctgacctcga ccgtcgaagc
     1081 ctgcaccttg acttgcgccg aggtggtgat ggtgacgcct gcgggttcga gcttgatgct
     1141 gttcccgctc gcgtccttga tctcgatgga gaggccgccg tccttgatct cgatggattg
     1201 gttctgcggc gtcttcaggg tcaaggtctc ggcgccctcg gtgtcgtcga gcgtgatgcg
     1261 gatgtcgcgg cgcgagacga tggacttctt gtagttgttg cccgcgccgt ccatctgctc
     1321 gggcggcgcg tccgagccgt tccagagcga gccgacgacg taagggcgcc gcgcgtcgcc
     1381 gccctcgaag acgacgagca cctcgtcgcc cacgtccggg atgaaccacg tgccgcggtt
     1441 gttcccggcc atcatcgtgg cgagccgcgc ccacacgtcg tagccgctgc cgtcgttgtc
     1501 gggcatccac ggcagcttga ccttgacgcg cccctgcgag tcggggtcgc ggatgtcggt
     1561 gacggtcgcc gggtaggcgc cgtagagccg catgcggtcg tgggccgcgt cgcattgttc
     1621 gaggacttcg agcacgatgc ctgtcaacct ccctcggtca accaccgatg ccgggccgct
     1681 cgacctcgaa agaggtgcgg tagccctgct cctgatcgaa cgtgtggcgc gcgagcgtca
//
LOCUS       DBNGOH010001115         4273 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1270487, whole genome shotgun sequence.
ACCESSION   DBNGOH010001115 DBNGOH010000000
VERSION     DBNGOH010001115.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4273)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4273)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4273
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1270487"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1102
                     /locus_tag="AC159P_21745"
     CDS             <1..1102
                     /locus_tag="AC159P_21745"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HIR3698123.1"
                     /translation="RFDLDKLEASPLFRSDANAYETVVALLDDAARHVITRREMPSAP
                     PNYYVRQLRGDARFNPNPASVGDKTTSLNLRALTDLNDATPQLQGITKRLVKYKRADG
                     VDLSFTLYLPPGYKEGTRLPTVVWAYPLEFNDPGTAGQITGSTQRYNSIVGISHLFFL
                     LEGYAILDNTTMPIVGDPEKMNDTYVEQIVSSAQAAIDKGVEMGVTDPERVGVGGHSY
                     GAFMTANLLAHSDLFRAGIARSGAYNRTLTPFGFQSERRTLWEAPETYIKVSPFMVAH
                     KINEPVLLIHGEADNNAGTYPIQSERLYQAIRGTGGTVRYVTLPLESHGYAARESVEH
                     TLHEMISWFDKHVKNAPPRTKREETTERGSRQ"
     gene            complement(1255..2790)
                     /locus_tag="AC159P_21750"
     CDS             complement(1255..2790)
                     /locus_tag="AC159P_21750"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HIR3698124.1"
                     /translation="MTHVILFSSEDAPSVRLLGALREAGIEVAATPEGDRAGFAGRGE
                     ETAAGPEGEGSPVAVVYEVACGADVAALYAEVARLSAEWPSLPLVACLRGKYGERLDA
                     LPTHELDAPLLRRLGFHAVAAEPVLLPALLQEIEAGGLPEDSGAGGEPEGEPEDGVDV
                     APASLLLPERLGTEKLRAAFEMVASLHFAADQKGAASAALAGLAALVRADRWTIYLVS
                     ETGRSEETTFEPLAARGLTASERALPEGDWRKALMGDALALTGAQSQAARAAVAYAEV
                     VRRKEGERRTVAVPLASGDRVTGVLEAVREGSDARPFSPAEASLLSALSLPLTAALTN
                     VVRVAEAERLSLTDDLTKLHNARFLRQYLTGELKRARRYGSMVTAMFLDLDDFKQIND
                     RNGHLVGSHVLMEMAAVILSSVRDTDVVARYGGDEFVVILPETNLEHATGVAERVRER
                     ISRHAFTGGRGLRLALTASFGVATFPLHAQSPQQLIAAADTAMYEAKAARKNCIRYAA
                     ETQ"
     gene            complement(2902..4146)
                     /locus_tag="AC159P_21755"
     CDS             complement(2902..4146)
                     /locus_tag="AC159P_21755"
                     /inference="COORDINATES: protein motif:HMM:NF016648.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HIR3698125.1"
                     /translation="MAMKISRFDYEWLIVPKRLIYIVIILVGLSLLAGGFGVYVWLHG
                     NPFKGAASEIGAPTGARFVSFEGGVRVVRAETREVLLARADTRLFPGDIVQTEEDGRA
                     RITLADGSTLLVKPNSVITIAENTAAEDGKSTKVRVAVESGQVNVRTEQQPEGAQNIV
                     GTQSTESRLAPETDASFDVREDKTEAIRVSTGAVETSTRSGDVTTLKGGEYVAVSQQG
                     SIAQKEKLLEAPAPSAPRNLERVAARRGGSTSVPLRWQRPATGAAAHYRVEVATSPFF
                     VAAGKVIERDQLQATEFTVDDLRQGNYFWRVRAMAPSGQASDWSEPQKFSVVVEGGTG
                     LSVTVADVTVEYVAGQIYLVRGRTQPGNTVRCAGRETLAARDGLFQLQINAPRGTREV
                     TVEAEDAQGNRNTARVPFTPTG"
BASE COUNT          682 a         1524 c         1412 g          655 t
ORIGIN      
        1 ccgcttcgat ctcgacaagc tcgaagcatc gcctctgttc cgcagcgacg cgaacgccta
       61 cgagaccgtc gtcgcgcttc tcgacgacgc cgcgcgccac gtcatcacgc ggcgcgagat
      121 gccgtccgcc ccgcccaact actacgtccg ccagctcagg ggcgacgcca ggtttaaccc
      181 caacccggct tcggtcggcg acaagacaac gagcttgaat ctgcgcgcgc tcaccgacct
      241 caacgacgcg acgccgcagc ttcagggcat cacgaagcgg ctcgtcaagt acaagcgcgc
      301 cgacggcgtt gacctctcgt tcacgctcta cctgccgccg ggctacaagg aagggacgcg
      361 gctgccgacc gtggtgtggg cctacccgct tgagttcaac gaccccggca cggcggggca
      421 gatcacaggc tcgacgcagc gctacaactc catcgtcggc atctcgcacc tcttcttcct
      481 gctcgaaggc tacgctatcc tcgacaacac gacgatgccc atcgtcggcg atcccgagaa
      541 gatgaacgac acctacgtcg agcagatcgt ctccagcgcg caggcggcca tcgacaaggg
      601 cgtcgagatg ggcgtcaccg accccgagcg cgtcggcgtc ggcggccact cctacggcgc
      661 cttcatgacg gcgaacctgc tggcgcactc cgacctcttc cgcgccggga tagcgcgcag
      721 cggcgcctac aaccgcacct tgacgccctt cggcttccag agcgagcgcc gcacgctctg
      781 ggaggcgccc gagacctaca tcaaagtctc gcccttcatg gtcgcccaca agatcaacga
      841 gccggtgctg ctcatccacg gcgaggccga caacaacgcc ggcacctacc cgatccagtc
      901 cgagcggctc taccaggcga tccgcggcac cggcggcacc gtccgctacg tcacgctccc
      961 gctcgaatcg cacggctacg ccgcccgcga gtccgtcgag cacacgctcc acgagatgat
     1021 ctcctggttc gacaagcacg tgaagaacgc cccgccccgc acgaagaggg aagagacgac
     1081 ggagagaggc agtaggcagt aactcggaag tcggaagtcg gaagtcggaa gagcgatgat
     1141 gcttgggagg taacgtccca agcctgtctg ctcgtcaagc aataaaggaa gaggcttgag
     1201 acgctacgtc tcaagcctct tcctttattc cgacttctga cttctgtttg ttgcctactg
     1261 cgtttcggcg gcgtagcgga tgcagttctt gcgggcggct ttggcttcgt acatggccgt
     1321 gtcggcggcg gcgatgagct gctgcggcga ctgcgcgtgg agcgggaagg tggcgacgcc
     1381 gaaggaggcc gtgagcgcga ggcgcaggcc ccggcccccc gtgaaggcgt ggcgggagat
     1441 gcgctcgcgg acgcgctcgg cgacgcccgt ggcgtgttcg aggttggtct cggggaggat
     1501 gacgacgaac tcgtcgccgc cgtagcgcgc gacgacatcg gtgtcgcgca ccgaagagag
     1561 gatgaccgcc gccatctcca tcaggacgtg cgagccgacg aggtggccgt tccggtcgtt
     1621 gatctgcttg aagtcgtcga ggtcgaggaa catcgccgtc accatcgacc cgtagcggcg
     1681 cgcgcgcttc agctcgcccg tcaggtattg acgcaggaag cgcgcgttgt gcagcttcgt
     1741 caagtcgtcc gtgagcgaca gccgctcggc ctccgcgacg cggacgacgt tcgtcagcgc
     1801 cgccgtcaga gggagcgaga gcgccgagag caaagacgcc tcggcgggcg agaacgggcg
     1861 cgcgtccgat ccctcgcgca ccgcttcgag cacgcccgtc acgcggtcgc cgctcgccag
     1921 cggcacggcc accgtgcggc gctcgccctc ctttcgccgc acgacctcgg cgtaagcgac
     1981 cgccgcgcgc gccgcctgac tctgcgcgcc cgtcagcgcc agcgcgtcgc ccatcaaagc
     2041 cttgcgccag tcgccttcgg gcagcgcccg ctcgctcgcc gtcagcccgc gcgccgccag
     2101 cggctcgaag gtcgtctcct cgctccggcc cgtctcgctg accagataga tcgtccaacg
     2161 gtcggcgcgc acgagcgccg ccagccccgc cagcgcagcc gaagccgcgc ccttctggtc
     2221 ggcggcgaag tgcagcgacg ccaccatctc gaaggccgcg cgcagcttct ccgtgccgag
     2281 ccgttcgggc aggagcaggc tcgcgggcgc gacatcgacc ccgtcctccg gctcgccctc
     2341 cggctcgccg ccggcgcccg agtcttccgg cagcccgccc gcctcgatct cttgcagcag
     2401 cgccgggagc agcaccggct cggcggcgac ggcgtgaaag ccgaggcgcc tcagcagcgg
     2461 cgcgtcaagc tcgtgcgtcg gcagagcgtc cagccgctcg ccgtacttgc cgcgcaggca
     2521 cgccacgagc ggcagcgacg gccactccgc cgacagccgt gcgacctccg cgtagagcgc
     2581 ggccacgtcc gccccgcacg ccacctcgta cacgaccgcg accggcgacc cttcgccctc
     2641 cggccccgcc gccgtctctt cgccgcgacc cgcgaagccc gcgcggtcgc cctccggcgt
     2701 cgccgcgacc tcgatccccg cctcgcgcaa agccccgagg agcctgaccg acggcgcgtc
     2761 ctcactgctg aagagaatca cgtgcgtcat aaaacttaag gactgaggac tgaggactga
     2821 ggtctgagtg aaagctttga ccgttgatga gaggagcgct gttcctactc agtcctcagt
     2881 cctcagtcct cggtacttta gtcatccggt tggtgtgaac gggacgcgcg cggtgttgcg
     2941 gttgccctgc gcgtcctcgg cttcgacggt gacttcgcgc gtgccgcgcg gcgcgttgat
     3001 ctgtagctgg aagaggccgt cgcgcgcggc gagcgtttcg cggcccgcgc agcggacggt
     3061 gttgcccggc tgcgtgcgac cgcggacgag ataaatctgg ccggccacgt actcgaccgt
     3121 cacgtccgcg accgtgacgc tcaagcccgt cccgccctcc acgacgaccg agaacttctg
     3181 cggctcgctc cagtcgctcg cctggcccga cggcgccatc gcgcgcacgc gccagaagta
     3241 gttgccctgc cggaggtcgt ccacggtgaa ctcggtggct tgcagttggt cgcgctcgat
     3301 gaccttgccc gcggcgacga agaagggcga ggtggcgacc tcgacgcggt agtgcgcggc
     3361 ggcgcccgtg gcgggccgct gccagcgcag cggcacgctc gtcgagccgc cgcggcgcgc
     3421 ggcgacgcgt tcgaggttgc gcggggcgga aggcgcgggc gcttcgagca gcttctcctt
     3481 ctgcgcgatg ctgccctgct gcgagacggc gacgtactcg ccgcccttga gcgtggtcac
     3541 atcgccgctg cgggtgctgg tttcgacggc gccggtgctg acgcggatgg cttcggtctt
     3601 atcctcgcgg acatcgaagc tggcgtccgt ttcgggcgcg aggcggctct cggtggactg
     3661 cgtgccgacg atgttctgcg cgccctcggg ctgctgctcg gtgcggacgt tgacctgacc
     3721 gctctcgacc gcgacgcgca ccttcgtgct cttgccgtcc tcggcggcgg tgttctcggc
     3781 gatggtgatg acgctgttcg gcttgaccag cagcgtcgag ccgtcggcga gcgtgatgcg
     3841 cgcgcgcccg tcctcctcgg tctggacgat gtcgccgggg aagagccgcg tgtcggcgcg
     3901 ggccagcagc acctcgcgcg tctctgcgcg cacgacccgc acgccgccct cgaacgagac
     3961 gaagcgcgcg ccggtcggcg ccccgatctc gctcgccgcg cccttgaacg ggttgccgtg
     4021 cagccagacg tagacgccga agccgcccgc gagcaaggag aggccgacca gaatgatgac
     4081 gatgtatatg agccgcttcg ggacgatcaa ccactcgtag tcgaagcgac tgatcttcat
     4141 tgccatacag agttacggac gaacgggaag aatcagcggg agcggcggca gagggtttcg
     4201 gggcggaaaa gatgcttgcg accgagggcg gcgtcgggat gaaatcgccg cacattcaaa
     4261 gtaacacaac gca
//
LOCUS       DBNGOH010001116         1992 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4385453, whole genome shotgun sequence.
ACCESSION   DBNGOH010001116 DBNGOH010000000
VERSION     DBNGOH010001116.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1992)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1992)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1992
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4385453"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            152..1594
                     /locus_tag="AC159P_21760"
     CDS             152..1594
                     /locus_tag="AC159P_21760"
                     /inference="COORDINATES: protein motif:HMM:NF017044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HIR3698126.1"
                     /translation="MVTETEDFRREQPAPLPERPLNLPVPAETRLANGLKLVVVEQDR
                     LPLVSFRLAFPRGDAFDPPELPGLTDVMTDMLVEGTESRTSRQIAEEVARYGATLSAG
                     ANSDYTTVAASSLSAYAEEILELLADVVLRPTFPEDELALSKANSQQNLIAQRAQPSF
                     LATEALARVIFGQHPYAVVAPTPESIDAMTRARLLDFHRSSFVAGGAVLIAVGDVKRD
                     AFVETAERLFGAWPARATAAPRFPAPPARDHRAAYVVDRPGSAQSNIVIANPGITRTH
                     PDYFNLLVMHTVLGANASSRLFMNLREEKGYTYGAYTTLDARRHAGTFRATAEVRTPV
                     TGDSLREFFHELGRIRDEDVGEQELRDAKTYLTGIFPIRLETQDGLVEQLVQMEMNGL
                     PADYLHTYRERVQQVGAAEVRRAAGDYVTPASAAIVIVGDAEAVTEQVRPYAERIEFF
                     DSAGRRKTEPDAAAAARVNGGEEDSATSRT"
     gene            1621..1935
                     /locus_tag="AC159P_21765"
     CDS             1621..1935
                     /locus_tag="AC159P_21765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3698127.1"
                     /translation="MNLSGTWNLEVSTPFGKHPATLVFERGGDGALNGHIDSRLGNAP
                     LLNLSTDGGGFDALVTMDFQGRGYEATIGGLADGDRIEGTIKVKLPIAPPLKFTGTRA
                     DS"
BASE COUNT          361 a          748 c          604 g          279 t
ORIGIN      
        1 aatgacgagc cggacggatg agaggcaccg tgcttttcat cgtccagcac aggacgatga
       61 aaagcacggt gcctctcatc atccgtcctg agtgacgaaa agcacgttgc ttctcattca
      121 tcattcaaca ttccgcattc atcattattt tatggttacc gagaccgaag acttccgccg
      181 agaacagccc gcgccgctgc cggagcgccc gctcaacctg cccgtccccg cagagacccg
      241 gctcgccaac ggcctgaagc tcgtcgtggt cgagcaggac agactgccgc tcgtcagctt
      301 ccgcctggcc ttcccgcgcg gcgacgcgtt cgacccgccg gagctgcccg gcctcacgga
      361 cgtgatgacc gacatgctcg tcgagggcac cgagtcgcgc acgagccggc agatcgccga
      421 agaggtcgcg cgctacggcg cgaccctgag cgcgggcgcc aactccgact acacgacggt
      481 cgcggcctcc tcgctctcgg cctacgccga agagattctg gagctgctgg cggacgtggt
      541 gctgcggccc acgttccccg aggacgagct ggcgctgtcg aaggcgaact cgcagcagaa
      601 cctgatcgcg cagcgcgcgc agccctcgtt cctcgccacc gaagccctcg cgcgcgttat
      661 cttcggccag cacccttacg ccgtcgtcgc gcccacgccc gagtccatcg acgcgatgac
      721 gcgcgcgcgc ctgctcgact tccaccgctc gtccttcgtc gccggcggcg ccgtgctcat
      781 cgccgtcggc gacgtgaaac gcgacgcgtt cgtcgagacc gccgaacgcc tcttcggcgc
      841 gtggcccgcg cgcgcgaccg ccgcgccgcg cttccccgcg ccccctgcgc gcgaccaccg
      901 cgccgcctac gtcgttgacc gccccggctc cgcgcagtcg aacatcgtca ttgccaaccc
      961 cggcatcacg cgcacgcacc ccgactactt caacctgctc gtcatgcaca cggtcttggg
     1021 ggccaacgcc tcctcgcgcc tcttcatgaa cctgcgcgag gagaagggct acacctacgg
     1081 cgcctacacg acgctcgacg cgcgccgcca cgccggcacc ttccgcgcga ccgccgaggt
     1141 gcgcacgccc gtcactggcg actcgctcag ggagttcttc cacgagctgg gccgcatccg
     1201 cgacgaagac gtgggcgagc aggagctgcg cgacgcgaag acctacctca caggcatctt
     1261 ccccatccgc ctcgagacgc aggacggcct cgtcgaacag ctcgtgcaga tggagatgaa
     1321 cggcctgccc gccgactacc tgcacaccta ccgcgagcgc gtccagcagg tcggcgccgc
     1381 cgaggtgcgg cgcgcggcgg gcgactacgt cacgcccgcg agcgcggcca tcgtcatcgt
     1441 cggcgacgcg gaggccgtca ccgagcaggt caggccgtac gccgaaagga tcgagttctt
     1501 cgacagcgcc gggcggcgca agacggagcc ggacgcggca gccgctgcgc gggtcaacgg
     1561 cggcgaagaa gattcggcca catcacgaac ttgataacga acgtgggaaa ggaaccgcac
     1621 atgaacctgt caggcacctg gaacctggaa gtctcgacgc ccttcggcaa gcacccggcg
     1681 acgctcgtct tcgagcgcgg cggcgacggc gcgctcaacg gccacatcga ctcgcggctc
     1741 ggcaacgcgc cgctcctcaa cctctcgacc gacggcggag gcttcgacgc cctcgtcacg
     1801 atggactttc agggccgcgg ctacgaagcc accatcggcg gcctcgccga cggcgaccgc
     1861 atcgaaggca ccatcaaagt caaactcccc atcgccccgc ccctcaaatt caccggcacc
     1921 cgcgccgaca gttgagaggg gcgaaaaggt tgaaagggga aaaggcgaaa agggaaagac
     1981 aacctctttc cc
//
LOCUS       DBNGOH010001117         3931 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1270634, whole genome shotgun sequence.
ACCESSION   DBNGOH010001117 DBNGOH010000000
VERSION     DBNGOH010001117.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3931)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3931)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3931
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1270634"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..631
                     /locus_tag="AC159P_21770"
     CDS             <1..631
                     /locus_tag="AC159P_21770"
                     /inference="COORDINATES: protein motif:HMM:NF013584.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HIR3698128.1"
                     /translation="VEVETLRVAFYTDNGVIAPTPDIAEAVRSAARALADAGATVEEA
                     RPPVLEQAFELVLGLFGADGGAGLRMLLHMSGTTETHALMDRLLELLGQRPLSTAEFG
                     GLAFQLDAYRSAMLSFMRDYDAILCPPCARAAMPHGTTFDEENYLSFTYTMNYNLTGW
                     PGAVVRAAASAEGLPLGVQLVARPWREDVALALARAVEAALGGWQRPPV"
     gene            693..1304
                     /locus_tag="AC159P_21775"
     CDS             693..1304
                     /locus_tag="AC159P_21775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HIR3698129.1"
                     /translation="MRQTSALSLPICWRIYMALCALVALSSVAAAQEAPRKKRNLARA
                     DRAAWREVLRWPETCEEDYESAYAGEEKYGGVEFHALGRGLYLVEVVCDGGGIQPSAV
                     FVLYDERRPRRARLLKLKGFDESDDAKRPARYSPVRALTKFDARRRELSLMSKYDAMG
                     TCGLFVRYRFVRGRPRVVEAREQVDCGGPEGTPDTDRWPRKRL"
     gene            1331..3382
                     /locus_tag="AC159P_21780"
     CDS             1331..3382
                     /locus_tag="AC159P_21780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014323785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HIR3698130.1"
                     /translation="MAKRSDAARVRAGVDTGGTFTDFVYRREGRARVFKLASTPADPS
                     AAIVEGLRTIARETGVALRDIEVVHGTTVGTNALLERRGARTALVTSKGFEDVLVIGR
                     QARGSLYDLDWERPAPLVSDALRFGVRERVGADGSIVEELADEEIAALVRRLKRAKAE
                     AVAVSLLFSFVRPEHERRIERALAAALEGVPLSVSHKILPEYREYERTSTVTINAYLQ
                     PLMGAYLARLSKRVPRGLRVMQSSGGSISAGVAADEPVRTILSGPAGGVVGATHAARG
                     AGFADIITFDMGGTSTDVALVAGGRLRLTSEAAVSGLPVAVPVLDIHTVGAGGGSVAR
                     VDAGGSLRVGPESAGADPGPACYGRGTLPTVTDANLVVGRFGGADLLGGDFKLDEGRA
                     VRALEGLAREMGRAGGREVSAAEAALGVVRVVNANMERALRAVSVERGHDPRAFALVS
                     FGGAGGLHAVELARGLRVSRVVVPLGPGALSAEGALRADVSKNLSRTVMLEVGGDGRT
                     ASIERAFRGMEREARAALRREGFGEARQRHERTVAARYKGQSFELEIAWDGGRRVVER
                     FHREHEARYGYAQGVASIEVVSARLRSAGLVEKSKDESPRRARGRAAAPHATAPVLFE
                     EGRLSVAVYRREELAAGARLEAPCVVTEYSSTTLVPPGARASVDARGNLIIEPGKGVG
                     F"
     gene            3524..>3931
                     /locus_tag="AC159P_21785"
     CDS             3524..>3931
                     /locus_tag="AC159P_21785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HIR3698131.1"
                     /translation="MTKRKNFDPTTLEIYRALYTSVAEEMGLTLRRTAHSPNIKERRD
                     YSCAVFDRGGRVIAQGDHMPVHLGSMPMAVAAALRETEVGPGDVVALNDPFAGGTHLP
                     DVTLVSGVFEGVLGAGDWVLGSTGRSRRSATSLK"
BASE COUNT          556 a         1280 c         1487 g          608 t
ORIGIN      
        1 ggtcgaagtc gaaacgctgc gcgtcgcctt ctacacggac aacggcgtca tcgcgccgac
       61 gccggacatc gcggaggcgg tgcgctccgc cgcacgcgcg ctcgcggacg cgggcgccac
      121 cgtcgaagag gcgcgaccgc ccgtgctcga acaggcgttc gagctggtgc tcggtctgtt
      181 cggcgcggac ggcggcgcgg gcctgcggat gctgttgcac atgtcgggca cgaccgagac
      241 gcacgcgctg atggatcgcc tgctcgaact gctcgggcag cgcccgctct cgaccgcgga
      301 gttcggcggc ctcgccttcc agctcgacgc gtaccgctcc gcgatgctct ccttcatgcg
      361 cgactacgac gccatcctct gcccgccctg cgcgcgcgcc gcgatgccgc acggcacgac
      421 cttcgacgag gagaactacc tctccttcac ctacacgatg aactacaacc tgacgggctg
      481 gcccggcgcg gtcgtgcgcg ccgccgcttc cgccgaaggt ctcccgctcg gcgtgcagct
      541 cgtcgcgcgt ccctggcgcg aggacgtggc gctcgcgctc gcgcgcgccg tcgaggcggc
      601 gctcggcggc tggcagcgcc cacccgtctg attgcggatt gcggattttg gattgcggat
      661 tgaagtcgag ttgccttcga cgccgtgacg gtatgagaca gacttcagct ctcagccttc
      721 caatctgttg gcgcatctac atggcgctgt gcgcgttggt cgccctctct tcagttgcgg
      781 cggcgcagga ggcgccgcgt aagaagagga acctcgcgcg cgccgaccgc gcggcttggc
      841 gcgaggtgct gcgctggccg gagacttgcg aggaagatta cgagagcgcg tacgcgggcg
      901 aagagaagta cggcggcgtc gaatttcacg ccctcggtcg cggcctgtac ctcgtggagg
      961 tggtttgcga cggcggcggc atacagccct ccgcggtctt cgtgctctac gacgagaggc
     1021 gcccgcgccg cgcgcggctg ctcaagctca agggtttcga cgagagcgac gatgcgaaac
     1081 ggcctgcgcg ttattcgccc gtgcgcgcgc tcacgaagtt cgacgcgcgg aggcgcgagc
     1141 tgtcgctgat gtcgaagtac gacgcgatgg ggacgtgcgg cctcttcgtc cggtacaggt
     1201 tcgtgcgcgg gcgtccgcgc gtggtcgagg cgcgcgagca ggtcgattgt ggcggccccg
     1261 aagggacgcc cgacacggat cgctggccga ggaagagatt gtagagaggc ggcggcgcgg
     1321 aagacagagg atggccaaga gaagcgatgc ggcgcgcgtg cgcgcgggcg tggatacggg
     1381 cgggacgttc accgacttcg tctaccggcg ggaggggcgc gcgcgcgtct tcaagctcgc
     1441 ctcgacgccc gcagaccctt cggcggcaat cgtcgaaggc ttgcgcacga tcgcgcgcga
     1501 gacgggcgtt gccttgcgcg acatcgaggt cgtacacgga acgaccgtcg gcacgaacgc
     1561 gctgctcgaa cgcagggggg cgcgcacggc gctcgtcacc tccaaagggt tcgaggacgt
     1621 gctcgtcatc ggtcggcagg cgcgcggctc gctgtacgac ctcgactggg aaaggcccgc
     1681 gccgctcgtc tcggatgcgc tgcgcttcgg cgtgcgcgag cgcgtcggcg cggacggctc
     1741 gatcgtcgaa gagctggccg acgaagagat cgcggcgctc gtccggcggc tgaagcgcgc
     1801 gaaggccgag gcggtcgccg tctctttgct cttctcgttc gtgcggcccg agcacgagcg
     1861 caggatcgag cgcgcgctcg cggcggcgct cgaaggcgtg ccgctctccg tgtcgcacaa
     1921 aatcctgccc gagtacagag agtacgagcg cacctcgacc gtcacgatca acgcttatct
     1981 gcaaccgctg atgggcgcgt acctggcgcg actgtcgaag cgcgtgccgc ggggcttgcg
     2041 cgtgatgcag tcttcgggcg ggagcatctc ggcgggcgtg gctgccgacg agccggtgcg
     2101 gacgattttg tctgggccgg cgggcggcgt cgtgggcgcg acgcacgcgg cgcggggcgc
     2161 gggcttcgcg gacatcatca cgttcgacat gggcgggact tcgacggacg tggcgctcgt
     2221 cgcgggcggg cgtctgcgtt tgacgagcga ggcggcggtg tcgggcttgc cggtggcggt
     2281 gccggtgctg gacatccaca cggtcggggc gggcggaggc tccgtggcgc gcgtggacgc
     2341 gggcggctct ctgcgggtcg ggccggagtc ggcgggcgcc gatccggggc cggcgtgcta
     2401 cgggcgcgga acgttgccga cggtgacgga cgcgaacctc gtggtcggtc gcttcggcgg
     2461 ggcggactta ctgggcggcg acttcaagct cgacgagggg cgtgctgtcc gtgctctcga
     2521 aggtctcgcg cgagagatgg gccgggcggg cgggcgcgag gtgtcggcgg cggaggcggc
     2581 gctcggcgtc gtgcgcgtgg tgaacgcgaa catggagcga gcgctgcgcg ccgtgtcggt
     2641 cgagcgcggc cacgacccgc gtgcgttcgc gctcgtgagc ttcggcggcg cgggcggcct
     2701 gcacgcggtc gagctggcgc gggggttaag agtctcgcgc gtcgtcgtgc cgcttggccc
     2761 cggcgcgctg tcggcggagg gggctttgcg cgcggacgtg tcgaagaacc tgagccgcac
     2821 ggtgatgctc gaagtgggcg gcgacgggcg aaccgcgtcg atcgagcgcg cgttccgcgg
     2881 gatggagcgc gaggcgcgcg cggccttgag gcgcgagggc ttcggcgaag caaggcagcg
     2941 gcacgagcgc acggtcgccg cgcgctacaa gggccagtcg ttcgagcttg agatcgcgtg
     3001 ggacggcggg cggcgcgtcg tcgagcgctt ccaccgcgag cacgaggcgc gctacggcta
     3061 cgcgcagggc gtcgcctcga tcgaagtcgt gagcgcgcgc ctgcgctccg ccggtctcgt
     3121 cgagaagtcg aaagacgaaa gcccgcggcg cgcgcgcggt cgcgccgccg cgccgcacgc
     3181 gacggcgccc gtgctcttcg aggaggggcg cctctcggtc gccgtctacc ggcgcgaaga
     3241 gctggcggcg ggcgcgcggc tcgaagcgcc ctgcgtcgtc acggagtatt cgtcaacgac
     3301 gctcgtcccg ccgggcgccc gcgcctcggt tgacgcgcgc ggcaatctca tcatcgagcc
     3361 ggggaagggt gttgggttct aggtgttggg tgttggattg gatgggcatt cggcgttaag
     3421 cattcagcac tcagcctgtc tttgtcgaat gctgaacgcc gaacgccgag ttcttttaac
     3481 ccaacaccca acacctagaa cccaacaccc gatttaaaag gcgatgacga aacgcaaaaa
     3541 cttcgacccg accacgctgg aaatctaccg cgcgctctac acgtcggtcg ccgaagagat
     3601 ggggctgacg ctgcggcgca cggcgcactc gccgaacatc aaggagcggc gcgactactc
     3661 gtgcgcggtc ttcgaccggg gcggcagagt catcgcgcag ggcgaccaca tgcccgtcca
     3721 cctcggctcg atgccgatgg ccgtggccgc cgccctgcgc gagaccgagg tcggccccgg
     3781 cgacgtcgtc gccctcaacg accccttcgc cggcggcacg cacctgcccg acgtaacgct
     3841 cgtctccggc gtcttcgaag gggtgttggg tgctggggac tgggtgttgg ggagtacagg
     3901 acgctcacgc cgctccgcga cctccttaaa g
//
LOCUS       DBNGOH010001118         2744 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4020631, whole genome shotgun sequence.
ACCESSION   DBNGOH010001118 DBNGOH010000000
VERSION     DBNGOH010001118.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2744)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2744)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2744
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4020631"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(30..584)
                     /locus_tag="AC159P_21790"
     CDS             complement(30..584)
                     /locus_tag="AC159P_21790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3698132.1"
                     /translation="MRRIRLALLCAAAVLTVGVTTYLVAANGQHAHKRGANAPGADSG
                     PLSSATVSFGAWMTSPPLDRFSNVPPPPPTNHHVVLPQVAKIKAGGAVNFIISGLHNI
                     AVYDDGTKPEDINAGLLDTAPPVPVIDDPNRRIYRGLDPRKLLPVLDRVEVVHFDEPG
                     TYLVICGVLPHFQEGMYGYVKVVP"
     gene            complement(878..>2744)
                     /locus_tag="AC159P_21795"
     CDS             complement(878..>2744)
                     /locus_tag="AC159P_21795"
                     /inference="COORDINATES: protein motif:HMM:NF025910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TPR end-of-group domain-containing protein"
                     /protein_id="HIR3698133.1"
                     /translation="SLSDVYSLGVLLYELLTGRRPYRFRSRLPHDVALAISEQEPERP
                     STAVTRTDNLTPAHHASHQPLTAELLAELRGAAPDELRLLLAGALDNVELKALRKDPA
                     ERYQSAAALREDINNYLEGRPVNAPAYLPPAPVFFAPAPAIVTPPAVRPIGASPKSVA
                     VLPLKLLDTGTGGTDTDDSYLGVGLADTLITRLSNVRRFVVRPTASVLRFADQQDPFA
                     AGRELAARYVLDGYIRRAGGRLRVTLQLLDVEHEAAVWAEQFGSDDGDVLELEDSIAE
                     RVARALVPQLSGEEQRQISKRGTDDPEAYEVYLRGRFHWSLMTEEGFAKAIGYYERAV
                     ELDPSYALAYTAIVEYYIFLGVQCVLPFAECARRATAAAERAAALDPSLAEARAALGV
                     VAINQEFDWAKAERYASRAAEMNPNYFMANQWLKTLYLQTGRFDEALKQARRLLDIAP
                     DSMMSLHFLAWTHHCSRRLEDSISAHRQLIRDAPHYAFGRLSFSWTLRCAGEFEEAVA
                     QARKGVELAPASPLYLSGLAAAEAGAGRLLESRRALGEIVEMSAARYVPPYMLAIVYL
                     ALGERSRALDQLERAFAIRDVWVVWLGVEPQFDPLRSDARFADLLRRMNHPLAPK"
BASE COUNT          417 a          885 c         1018 g          424 t
ORIGIN      
        1 gctgcgctca ggcacgcccc cgcgattcgt tagggcacga ccttcacgta cccgtacatc
       61 ccctcctgga agtgtggcag cactccgcag atgacgaggt acgtccccgg ctcgtcgaag
      121 tgcaccacct cgacccgatc cagcacgggg agcagcttac gcgggtccag ccccctgtat
      181 atcctccggt tcgggtcgtc gatcaccggc accggaggcg ccgtgtcgag caggcccgcg
      241 ttgatgtcct cgggcttcgt cccgtcgtcg tagacggcga tgttatgcag ccccgagatg
      301 atgaagttga ccgcgccgcc cgccttgatc ttggcgacct gcggcaacac cacgtggtgg
      361 ttggtgggcg gcggcggcgg cacgttcgag aacctgtcga gcggcggcga ggtcatccac
      421 gcgccgaagc tgacggtggc gctgctcagc gggccgctgt cggcccccgg cgcgttggcg
      481 cctctcttgt gtgcgtgttg accgttggcc gccacgaggt aagtcgtcac tccgacggtg
      541 aggacggcgg cggcgcaaag gagcgccagg cgtatgcgtc tcatcttctc ctcctcattc
      601 ttgagttcag tgggcgaggc tgcgggcttc ccgactgtgt cggaggcgcg gcagttccgc
      661 gcggggcaag tcactcgtgc agcagagaac gcaggcaggg cgcgcgcgcg aaggaatccc
      721 cggatgcggc tccgaaagga atggacggcg cccttctata acaggggcgg aggcaagtca
      781 aggtctcggc ggcgacgcca ctgacaagtg ccgcgacgac ccgcgccaca acgactgcgg
      841 gggaagctcc cactgcccgc cgctcaccgc tcaccgctca cttgggagcg agcgggtggt
      901 tcatgcggcg gaggaggtct gcgaagcgtg cgtcggagcg gagggggtcg aactgcggct
      961 cgacgccgag ccagacgacc cacacgtcgc ggatggcgaa ggcgcgttcg agctggtcga
     1021 gcgcgcggct gcgttcgccg agggcgaggt agacgatggc gagcatgtaa ggcgggacgt
     1081 agcgcgcggc ggacatctcg acgatctcgc cgagcgcgcg gcgcgattcc aagaggcgcc
     1141 cggcgccggc ctcggcggcg gcgagtccag agaggtagag cgggctcgcg ggcgcaagct
     1201 cgacgccttt gcgcgcctgc gcgaccgcct cctcgaactc gcccgcgcag cgcagcgtcc
     1261 aactgaaaga gaggcgaccg aaggcgtagt gcggcgcgtc cctgattaac tgccggtggg
     1321 cgctgatcga gtcttcgagg cggcgcgaac agtggtgcgt ccaggcgagg aagtgcaggc
     1381 tcatcatcga gtcgggcgcg atgtcgagca ggcggcgggc ttgcttgaga gcctcgtcga
     1441 agcggcccgt ctgcaagtag agggtcttca accactggtt cgccatgaag tagttggggt
     1501 tcatctcggc ggcgcgcgag gcgtagcgct cggccttcgc ccagtcgaac tcctggttga
     1561 tggcgacgac gccgagcgcg gcccgcgcct cggcgagcga ggggtcgagc gccgccgccc
     1621 tttccgccgc cgcggtcgcg cgccgcgcgc actcggcgaa cgggagcacg cactgcacgc
     1681 cgaggaagat gtagtactcg acgatcgccg tgtaggcgag cgcgtaagac gggtcgagtt
     1741 cgacggcgcg ctcgtagtag ccgatggctt tggcgaagcc ctcctcggtc atcaggctcc
     1801 agtggaagcg cccgcgcagg tagacttcgt aagcttcggg gtcgtcggtg ccgcgcttgg
     1861 aaatctgccg ctgctcttcg ccggagagct gcggcacgag cgcgcgcgcg acccgctcgg
     1921 cgatggagtc ttcgagttcg agcacatcgc cgtcgtcgct gccgaactgc tcggcccaga
     1981 ccgccgcctc gtgctccacg tcgagcaact gcaaggtgac gcgcagccga ccgcccgcgc
     2041 ggcggatgta gccgtcgagg acgtagcgcg cggccagctc gcgccccgcc gcgaaagggt
     2101 cttgttgatc cgcgaagcgc aagaccgagg ccgtcgggcg cacgacgaag cggcgcacgt
     2161 tggagagccg cgtgatgagc gtgtcggcca gaccgacgcc gaggtagctg tcgtccgtgt
     2221 ccgtgccgcc cgtgcccgtg tcgaggagct tgagcggcag caccgcgacc gacttcggcg
     2281 acgcgcctat ggggcggacg gcgggcggcg tcacgattgc gggcgcgggc gcgaagaaca
     2341 cgggcgcggg cggcagatac gcgggcgcgt tgacgggacg gccttcgagg tagttgttga
     2401 tgtcttcgcg tagcgccgcc gccgactggt agcgctcggc ggggtctttc cggagcgcct
     2461 tgagctcgac gttgtcgagc gcgcccgcga gcagcaggcg cagctcgtcg ggcgccgcgc
     2521 cgcgcaattc ggccagcagt tctgccgtca acggttgatg cgaggcgtgg tgtgcgggcg
     2581 tcaggttgtc cgtgcgcgtg acggcggtgc tcggtcgctc cggctcctgc tcggagatgg
     2641 cgagcgccac gtcgtgcggc aggcggctgc ggaagcggta aggtctgcgg cccgtcagca
     2701 gctcgtagag cagcacgccc aggctataca cgtcgctcag ggaa
//
LOCUS       DBNGOH010001119         2980 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4201520, whole genome shotgun sequence.
ACCESSION   DBNGOH010001119 DBNGOH010000000
VERSION     DBNGOH010001119.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2980)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2980)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2980
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4201520"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(108..1292)
                     /locus_tag="AC159P_21800"
     CDS             complement(108..1292)
                     /locus_tag="AC159P_21800"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012259064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HIR3698134.1"
                     /translation="MREAVIVAGVRTAVGKAPRGSLRTTRPDDMAAAVIEDLLRRVPA
                     LPPESIDDVMLGCATPEGESGTNVARIASLRAGLPQEVPAVTVNRFCSSGSQTIAMAA
                     ASIMAGRAECVIAGGTESMSMLRGNTRFRPNPFLVEHRPGVYLSMGLTAENVARDYKV
                     SREEADAFSYRSHRRALAALAEGKFKDDGIVPLEVEIKEVGGDGRVKQKMFTFDADEG
                     PRPDTTPEGLAKLRPVFHARGTVTAGNSSQTSDGAAGVVVMSRERADELGIKPLARFV
                     SYAVGGVAPEIMGMGPVAAVPKALKLAGLELEDIDLIELNEAFACQAIAVMRELGMDD
                     ERTNVNGGAVALGHPLGASGAKLTVQLLGEMRRRKSRYGLVTMCVGGGQGAATVFENL
                     QL"
     gene            complement(1341..1724)
                     /locus_tag="AC159P_21805"
     CDS             complement(1341..1724)
                     /locus_tag="AC159P_21805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008297815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HIR3698135.1"
                     /translation="MKVGDVVAWERTFTEEDVRLFARFSGDEGEHHLQPDETGRLMVH
                     GLLTATLPTKIGGDINFLARQLTFRFHRPVFSGDTVRCEVTIEELTAAEQYTHLKSSW
                     VCRNQHGKEVMSGEAHGVIRGADGG"
     gene            complement(1754..2236)
                     /locus_tag="AC159P_21810"
     CDS             complement(1754..2236)
                     /locus_tag="AC159P_21810"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014395325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0047617 - fatty acyl-CoA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HIR3698136.1"
                     /translation="MTRTTDEEKPSIDTEARRDLTGLEYMRQLMTGEREPSGMARLMN
                     MRLVEVEEGRAVFAVEPDERHYNGLGIAHGGLAATLLDSALGCAINTMMTGGRVFTTL
                     EMKVNYVRPLTAETGEVRCEAQVLHVGGRVATAEGRLVDARGKLYAHGTATCRLFRTR
                     "
     gene            complement(2518..>2980)
                     /locus_tag="AC159P_21815"
     CDS             complement(2518..>2980)
                     /locus_tag="AC159P_21815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase/enoyl-CoA
                     hydratase family protein"
                     /protein_id="HIR3698137.1"
                     /translation="YMGLVEVGVGLIPAAGGCKEFVRRVVSPPLRAAANADPVPFLQQ
                     ALQTIATAKVSTSAAEARALGYLTDADLVVMNRDHQLAEAKRLVLELADAGYAPPARA
                     AKNCYAAGRDALAALRAGLYVMQQGGYMSEYDLHVSLKVAHVLAGGDLSSA"
BASE COUNT          443 a         1112 c          904 g          521 t
ORIGIN      
        1 gaggaagcgt ctcattcgag attcgagcat cgacttctcc gcgccctccg cgcctccgcg
       61 ttccctgcgt tgaaactgcc gcgcgaagca ttcaatcacg catcacatca aagctgaagg
      121 ttctcgaaga cggtcgccgc gccctgcccg ccgccgacgc acatcgtcac cagcccgtaa
      181 cgcgacttgc gccgccgcat ctcgcccaaa agctggacgg tcagcttcgc gcccgacgcg
      241 ccgagcgggt ggccgagcgc gaccgccccg ccgttcacgt tcgtgcgctc gtcgtccatc
      301 cccagctccc gcatcaccgc tatcgcctga cacgcgaacg cctcattgag ttcaatcagg
      361 tcgatgtctt cgagttcgag gccggcgagc ttcagcgctt tgggcaccgc cgctacaggc
      421 cccatcccca tgatctccgg cgcgacgccg cccaccgcgt acgagacgaa acgcgccagc
      481 ggctttatgc ccagctcgtc tgccctctcg cgcgacatga cgacgacgcc cgccgcgccg
      541 tccgaagtct ggctcgaatt gcccgccgtc acggttccgc gcgcgtggaa gacggggcgc
      601 agtttggcta gcccttccgg cgtcgtgtcg ggccgcggcc cctcgtccgc gtcgaacgtg
      661 aacatctttt gcttcacgcg accgtcgccg ccgacctctt tgatctccac ttcgagcggg
      721 acgatgccgt cgtccttgaa cttcccctcc gccagcgccg cgagcgcgcg ccggtggctg
      781 cggtaagaga aggcgtccgc ctcctcgcgc gagaccttgt aatcgcgcgc cacgttctcc
      841 gccgtcagcc ccatgctcaa atagacaccg ggccggtgtt cgaccaagaa agggttcggc
      901 ctgaacctgg tgttgccgcg cagcatgctc atcgactcgg tgccgcccgc gatgacgcac
      961 tcggccctgc ccgccatgat cgaggcggcg gccatcgcga tggtctgcga ccccgaagag
     1021 cagaagcggt tgaccgtcac cgccggcact tcctgcggca gccccgcgcg caaacttgcg
     1081 atgcgcgcga cgttcgtgcc cgactcgccc tcgggcgtgg cgcagcccag catcacatcg
     1141 tcgattgatt cgggcgggag cgcgggcacg cgccgcagca ggtcttcgat gaccgccgcg
     1201 gccatgtcgt ccggcctcgt cgtgcgcagg ctcccgcgcg gcgccttgcc gaccgccgtc
     1261 cgcacgccgg ccacgatcac agcctctctc atgcgccgtc ctcccgtctg caaaaatgcc
     1321 cctcggccaa tcgagtccgg tcagccgccg tcggcgccgc ggatgactcc gtgcgcctcg
     1381 ccgctcatca cctctttgcc gtgctggttg cggcagaccc acgaagactt cagatgcgtg
     1441 tactgttccg ccgccgtcag ctcttcgatc gtcacctcgc agcgcaccgt gtcgccgctg
     1501 aagacggggc ggtgaaaacg gaacgtcaac tggcgcgcga ggaagttgat gtcgccgccg
     1561 atcttcgtcg gcagcgtcgc cgtcagcagc ccgtgcacca tcagcctgcc cgtctcgtcc
     1621 ggctgtaagt ggtgctcccc ctcgtcgccc gaaaatcttg cgaacaggcg cacgtcctct
     1681 tccgtgaacg tccgctccca ggctaccacg tcgccgacct tcatccccac accttcacct
     1741 tcggtgtccg accttaacgc gtgcggaaca gcctgcacgt cgcggtgccg tgcgcgtaga
     1801 gcttcccgcg cgcatcgaca agacggcctt cggcggtggc gacgcgcccg ccgacgtgga
     1861 gcacctgcgc ctcgcagcgc acctcgcccg tctccgccgt caaagggcgc acgtagttga
     1921 ccttcatctc aagcgtcgtg aagacccgcc cccccgtcat catcgtgttg atggcgcagc
     1981 ccagcgccga gtcgagcagc gtcgccgcca gccctccgtg cgcgatgccg agtccgttgt
     2041 agtggcgctc gtccggctcg acggcgaaga ccgcgcgccc ctcttccacc tcgacgagcc
     2101 gcatgttcat cagccgcgcc atccccgaag gctcgcgctc gcccgtcatg agctggcgca
     2161 tgtactcaag cccggtcagg tctcggcgtg cttccgtgtc gatggacggt ttctcttcgt
     2221 cggtcgtgcg cgtcatccct ctcgcacctc tcgtttcaac ctctgccgat ggttatagct
     2281 gtaggggcag ggctggtccc tgcctgcgag ttgcatattc attcggcgtc gaagctatcg
     2341 aagcgcaacg ggccgcggac tggccctgcc ccctacagtt ttgcgaccgg ttgttcagtt
     2401 ccgcaaaggc ttccccgtag ttagcatgtg cgtgatgcgc tcgatggtct tcggctcgcc
     2461 gcagagcgag acgaacgcct cgcgctcaag gtcgaggaag tattgctctt cgacccacta
     2521 ggcggaagag aggtcgccgc ccgcgaggac gtgcgcgacc ttgagactca cgtgcaggtc
     2581 atactcgctc atgtaaccgc cctgctgcat gacgtagagg cccgcgcgca gggcggcgag
     2641 cgcgtcgcgc cccgccgcgt agcagttctt cgccgcgcgc gcgggcggcg cgtagcccgc
     2701 gtccgcgagt tcgagcacga gccgcttcgc ctcggccaac tggtggtcgc ggttcatcac
     2761 gacgaggtcc gcgtcggtca ggtacccgag agcgcgcgcc tcggcggcgc tcgtcgagac
     2821 cttcgccgtg gcgatggtct ggagcgcctg ctgcaggaaa ggcaccgggt cggcgttcgc
     2881 ggccgcgcgc agcggcggcg agacgacgcg gcgcacaaac tccttgcaac cgcccgcggc
     2941 cgggatgagc cccacgccga cctcgaccag ccccatgtag
//
LOCUS       DBNGOH010001120         8269 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2185626, whole genome shotgun sequence.
ACCESSION   DBNGOH010001120 DBNGOH010000000
VERSION     DBNGOH010001120.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8269)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8269)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8269
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2185626"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            174..4661
                     /locus_tag="AC159P_21820"
     CDS             174..4661
                     /locus_tag="AC159P_21820"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HIR3698138.1"
                     /translation="MNPEREKQVDRLFESARALAPAERAAYLEEACAGDRELREQVEA
                     LVAYHERGAGEETALPREAAGLGARAAAAQAEAEDLAPGRAFGSYMILGKLGEGGMGT
                     VYLAQDVRLGRKVALKFLPARYAGDEVFVRRFVQEARAASQLNHPNILVVHEIGQHEG
                     RHFIATEFIEGDTVRRLLAHGPLRVEEAIDVCAQVAAALTKAHQNGVVHRDIKPENVM
                     VGEERHVKVLDFGIAKQLHASTIVDTEAPTSARLDTAPGVVLGTSAYMSPEQVRGFEV
                     DARTDVWSLGCVLYEMLAGRTPFDAPTHGDLIVAVLNEEPPPLARWRADVPEDLARVV
                     SKALAKRKESRYQSARAFQADLVRLRQSLQLLPGAAHGADTASHPGPSDRGPRPTQGP
                     SGFAHTFTTGGQRRQVTILFADLAGLASLTEGLDAEEVGELLADLWPRVDAALTEHGG
                     AIDKHVGDAVVALWGAAGAAREDDPERAIRAALAIEETVGEFAAANLHDTAPTRLKRE
                     GEDEGAKGPPLVRAGVSTGLVLVAPAGGAGGGVTATGEAVSLASRLAQSAPVGAILVT
                     HDTYRHVRGVFDVRAPVTFKLEGKSAPVEIYRVLRTKPRAFRQRTRGVEGVETRMIGR
                     RGELRLLTDTLQAVFEERELQVVTVVADAGLGKSRLLYEFANRVELLPEEWYIFNGRG
                     SEATQGLPYALLRDVFSFRFEIQDSDPPAVAREKLERGMTEMVGGDGEEASMRAHFVG
                     QLIGLDFSDSRHLSGILDDARQIRDRAFRYAAQFFAEVSRSGPIVLYFDDIHWADDGS
                     LDFIPYLARNCAQVPMLVLCLARTSLLERRPAWGEGEEAHALVRLQPLSKKESRQLVE
                     EILRHAPNVPERLRDLVVSGAEGNPFYVEELIKMLIDQRVIVPGADEWSVDANRLGEV
                     KVPPTLTGVLQARLDNLTPREYAALQRASVVGRVFWDSAVEHLGADSAETRVGEPPHA
                     EISAAVAEVLESLRRKELIYRREGSSFAGASEYLFKHTLLRDVTYESVLRRERRAYHR
                     RVAEWLVSKSGGRVAEHAAHVAEHYEQAREERHAARWYARAGQQARETYAPEAAILYY
                     RKALEFPPPLSSSTDEEPGMGARRAAAVALYEGLGEVLRVQARYAESIEAYGRMREAA
                     AALGDRATEARAWNEVALVLASQGDNRSTLDAARRAEALAREAGDDEQARVEMAHALS
                     LQSQASTRLGDARAAMRLATQALTYATQLGEEGRRVAADSLKSLGMAHHILGNFERAE
                     HYKEQALHTDRERGDRRGVGNLLNSLGETARLRGDYEAAFRRYQEALAIAREIGNRNG
                     EILYLSNLGAARTGLGDYAAAASDLRQTIELASAAGYFGLSENYRFLAEALLGQGQTE
                     AALEAAHKALELGRELENQEHIGEAWRVLGLVASHSSLALLTVGGLAFDAAACFAESL
                     RVFAAIKMESERARTLREWARHELARGDRDRGREMWREARDIFTKLGMGLEVARMPGG
                     E"
     gene            complement(4736..6805)
                     /locus_tag="AC159P_21825"
     CDS             complement(4736..6805)
                     /locus_tag="AC159P_21825"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013862.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HIR3698139.1"
                     /translation="MSLQDPAPNPDRDPAAAPAEEEAAPARRELEGAELLVKIIFGVI
                     FVLLVTSAWAFICTGSQKGWMVALKFIAWAVIFGAFVVNAWKQYVRPIGTWVALGLLL
                     SFAWLAWAGSYHRTRDLLCMGFSFGAVMLLLPRGRLWLKSLYAVIKECAEESVGTGRP
                     VIPNFTKHRPLQGAWGFIFFVSFIFLFWLTSSGLNAWNVGAPVPTDPARFAALHEKSP
                     WRDVRVGVALSGGGYRAVLMHAGVLDAFETLGVPVTHMTSVSGGSITGAFYAAGGEPR
                     RLRAAINERRFNLTRNMTDAQNALRLPFPFKLPWTDVKLFKWYSFTRSDVQSALLDSV
                     LLDQKKLSDLKRGAGAPRLMICTTDLYSGSFLGFSEEGVLRRFMIQAKEKDDFINVKA
                     PRDEEKSVFFAVSDEEFPRREYLSRLVAASGAFPGAFNAVSEEMYLDRPGGNAQKKRP
                     VLLADGGITDNSALTMLLFAPTKLEDWKVDVAVSSDASALFAEQSDFTALGELSRAID
                     IVYANVGVRRFRQESRSPQILLSPASFLDTRLTDEGERRAQIKAVFDEGVSRLSPEAL
                     RVLVPDISDQAQRREAEELFARCCEDEAAIGRIKEILSADLHESVGAFLRASTLRDNF
                     DGDDANKIFRLGQYLVALNWSYLRAKLDEAKAAKRGGGQPAPPAETVLQVGGPAGAAA
                     EMTLKRR"
     gene            complement(7380..>8269)
                     /locus_tag="AC159P_21830"
     CDS             complement(7380..>8269)
                     /locus_tag="AC159P_21830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome B6"
                     /protein_id="HIR3698140.1"
                     /translation="PAIFLTTRPDLGDVSKGQLVTVENFYELFNGILNPKQLDGLRLL
                     VTQFPQQQFNATADRKTERADGMLGVTCFDCHVNGHTLGAAHLVGDIRPQENRRRIET
                     PSLRGVNVQRLFGSQRALKTVEDFTEFEQRAAYFDGDPVTAAKKGVNVLDRGLHVHAM
                     AEFQELLDFPPAPKLNVYGRLDRRKASPAEVRGEELFFGKANCATCHTPPFYTDGNMH
                     DLQVERFFKPRMEGGMMITKQGPIKTFPLRGIKESPPYLHDGRLLTLEDTVEFFNLVL
                     ELNLAQTEKSDLVAFLRAL"
BASE COUNT         1279 a         2893 c         2927 g         1170 t
ORIGIN      
        1 ccgcctaccg cctactgcct acttcgtttg tgttacaatc cgcgctcgtc tgccttgatg
       61 ttttgaccag ccgccgcgag acaggtggag gcagttgtgc cctcacccac gccgtatgat
      121 cgagtcccgc cgcatgaagc cgggcggggc cggagcaagc aggccacacg ggaatgaatc
      181 ctgagcggga gaaacaggta gaccgcctct tcgagtccgc gcgcgcgctc gcgcccgcgg
      241 agcgcgcggc ctatctcgaa gaggcgtgcg ccggtgaccg agagctgcgc gaacaggtcg
      301 aggcgctggt cgcctaccac gagcgcggcg cgggcgagga gaccgccctg ccgcgcgagg
      361 ccgcaggact cggggcgcgg gcggcggcgg cgcaggccga ggcggaagac ctcgcgccgg
      421 ggcgcgcgtt cggctcgtac atgatcctcg gcaagctcgg cgagggcggc atgggcacgg
      481 tctatctggc gcaggacgtg cggctcggtc gcaaggtcgc gctgaagttc ctgcccgcgc
      541 gctacgccgg cgacgaggtc ttcgtccgcc gcttcgtgca ggaggcgcgc gccgcctcgc
      601 agctcaacca cccgaacatc ctcgtcgtcc acgagatcgg gcagcacgag ggccgccact
      661 tcatcgccac cgagttcatc gagggcgata ccgtcaggcg cctcttggcg cacgggccgc
      721 tccgcgtcga ggaggcgatc gacgtgtgcg cgcaggtcgc cgcggcgctg acgaaggcgc
      781 accagaacgg cgtcgtccac cgcgacatca agccggagaa cgtgatggtc ggcgaggagc
      841 ggcacgtcaa ggtcttggac ttcggcatcg ccaagcagct ccacgcgagc acgatcgtgg
      901 acaccgaggc gcccacgagc gcgcgcctcg acacggcgcc gggcgtcgtg ctcggcacct
      961 ccgcctacat gtcgcccgag caggtgcgcg gcttcgaggt ggacgcgcgc accgacgtgt
     1021 ggagtttggg gtgcgtgctc tacgagatgc tcgcgggccg cacgcccttc gacgcgccga
     1081 cgcacggcga cctcatcgtc gccgtcctca acgaagagcc gccgccgctc gcgcgctggc
     1141 gcgcggacgt gcccgaagat ctggcgcgcg tcgtctcgaa ggcgctcgcc aagcgcaagg
     1201 agagtcgtta ccaatcggcg cgcgcctttc aggcagacct cgtgcggctc cgccagagtc
     1261 tccaactgct gccgggcgcg gcccacggcg ccgacacggc ttcgcacccc ggcccctccg
     1321 accgcgggcc gcggcccacg caaggcccga gcgggttcgc gcacacgttc acgaccggcg
     1381 ggcagcggcg gcaggtgacc atcctcttcg ccgacctcgc gggcctcgcc tcgctcaccg
     1441 agggcctcga cgccgaggag gtcggcgagc tgctggcaga cctctggccg cgcgtggacg
     1501 cggcgctgac cgagcacggc ggcgccatcg acaagcacgt cggcgacgcg gtggtcgcgc
     1561 tgtggggcgc ggcgggcgcc gcgcgcgagg acgaccccga gcgcgccatc cgcgccgcgc
     1621 tcgccatcga agagaccgtc ggcgagttcg ccgccgcgaa cctgcacgac acggcgccga
     1681 cacggttgaa gcgcgagggc gaagacgagg gggcgaaggg gccgccgctc gtgcgcgcgg
     1741 gcgtgagcac ggggttggtg ctcgtcgccc ccgcgggcgg cgcgggcggc ggggtgacgg
     1801 cgacgggcga ggccgtgagc ctcgccagcc ggctcgcgca gtcggcgccg gtcggcgcca
     1861 tcctcgtcac gcacgacacc taccgccacg tgcgcggcgt cttcgacgtg cgcgcgcccg
     1921 tcaccttcaa gctcgaaggc aagtcggccc cggtcgaaat ctaccgcgtg ctccggacca
     1981 agccgcgcgc cttccgccag cgcacgcgcg gcgtcgaggg cgtcgagacg cgcatgatcg
     2041 ggcgccgcgg cgagctgcgg ctgttgaccg acacgctgca agcggtcttc gaagagcgcg
     2101 agcttcaggt cgtcacggtc gtcgccgacg ccggcctcgg caagtcgcgc ctgctgtacg
     2161 agttcgccaa ccgcgtcgag ctgctgcccg aagagtggta catcttcaac gggcgcggca
     2221 gcgaggcgac gcaggggctg ccctacgcgc tgctgcgcga cgtgttctcg ttccgcttcg
     2281 agatacagga cagcgacccg cccgcggtgg cgcgcgaaaa actcgaacgc ggcatgaccg
     2341 agatggtcgg cggcgacggc gaggaggctt cgatgcgcgc gcacttcgtc ggccagctca
     2401 tcgggctgga cttttccgac agccgccatc tctcgggcat cctcgacgac gcgcgccaga
     2461 tccgcgaccg cgccttccgc tacgccgcgc agttcttcgc agaggtctcg cgctccggcc
     2521 ccatcgtcct ctacttcgac gacatccact gggccgacga cggctcgctc gacttcatcc
     2581 cctacctcgc gcgcaactgc gcgcaggtgc cgatgctcgt cctctgcctc gcgcgcacct
     2641 cgctgctcga acggcggccc gcgtggggcg agggcgaaga ggcgcacgcg ctcgtgcggc
     2701 tccagccgct ctcgaagaag gagagccgcc agctcgtcga agagattctg cggcacgcgc
     2761 cgaacgtccc cgaacgcctg cgcgacctcg tcgtctccgg tgccgagggc aacccgttct
     2821 acgtcgaaga gctgatcaag atgctcatcg accagcgcgt catcgtgccg ggcgccgacg
     2881 agtggagcgt ggacgcgaac cggctcggcg aggtgaaggt gccgccgacc ctgacgggcg
     2941 tgcttcaggc gcggctcgac aacctcacgc cgcgcgagta cgcggcgctg caacgcgcct
     3001 cggtcgtggg ccgcgtcttc tgggacagcg ccgtcgaaca cctcggcgcc gactccgccg
     3061 agacgcgcgt cggcgagccg ccgcacgcgg agatcagcgc ggcggtcgcc gaagtgctcg
     3121 aatccctgcg ccgcaaggag ctgatctacc ggcgcgaggg gtcgagcttc gcgggcgcga
     3181 gcgagtacct cttcaagcac acgctgctgc gcgacgtgac ctacgagagc gtgctccggc
     3241 gcgagcgccg cgcttatcat cggcgcgtcg ccgagtggct cgtctcgaag agcggcgggc
     3301 gcgtcgccga gcacgcggcc cacgtcgccg agcactacga gcaggcgcgc gaggagcgcc
     3361 acgccgcgcg ctggtacgcc cgcgccggcc agcaggcgcg cgagacctac gcgcccgagg
     3421 ccgccatcct ctactaccgc aaggcgctcg aattcccgcc gccgctctcc tcgtccacgg
     3481 acgaagagcc gggcatgggc gcgcgccgcg ccgccgcggt cgcgctctac gagggtctgg
     3541 gcgaagtgct gcgcgtgcag gcgcgctacg ccgaatccat cgaggcttac gggcggatgc
     3601 gcgaggcggc ggcggcgctc ggcgaccgcg cgacggaggc gcgcgcgtgg aacgaggtgg
     3661 cgctcgtgct ggcgagtcag ggcgacaacc gctcgacgct cgacgccgcg cgccgcgccg
     3721 aagcgctcgc gcgcgaggcc ggagacgacg agcaggcgcg cgtcgagatg gcgcacgcgc
     3781 tcagcctgca aagtcaggcg agcacgcgcc tcggcgacgc gcgcgccgcg atgcggctcg
     3841 ccacgcaggc gctcacctac gcgacccagc tcggcgaaga gggccgccgc gtcgccgccg
     3901 acagcctcaa gtcgctcggc atggcccacc acatcctcgg caacttcgag cgcgccgagc
     3961 actacaagga gcaggcgctc cacaccgacc gcgagcgcgg cgaccgccgc ggcgtcggca
     4021 accttctcaa cagcctcggc gagacggcgc gcctgcgcgg cgactacgag gccgccttcc
     4081 gccgctatca ggaggcgctc gccatcgcgc gcgagatcgg caaccgcaac ggcgaaatcc
     4141 tctacctctc caacctcggc gccgcgcgca cgggcttagg cgactacgcc gccgccgcga
     4201 gcgacctgcg gcagacgatc gagctggcga gcgccgccgg ctacttcggc ctctccgaga
     4261 actaccgctt cctcgccgag gcgctgctcg ggcaggggca gaccgaggcc gcgctcgaag
     4321 ccgcgcacaa ggcgctcgaa ctcgggcgcg agcttgagaa ccaggagcac atcggcgagg
     4381 cctggcgcgt cctcggcctc gtcgcctcgc actcttcgct ggccctcctg acggtcggcg
     4441 gcctcgcctt cgacgccgcc gcctgcttcg ccgaaagcct ccgcgtcttc gccgccatca
     4501 agatggagtc cgagcgcgcc cgcaccctgc gcgaatgggc gcgccacgaa ctcgcccgcg
     4561 gcgaccgcga ccgcgggcgc gagatgtggc gcgaggcgcg cgacatcttc acgaaactcg
     4621 ggatggggtt ggaagtcgcg cggatgcccg gcggggagtg aggcggggcg cggtcgagtc
     4681 aacgtcggga tgacgggggc ggcgggccgg cccgccgcgc gagacgcagg agccgtcacc
     4741 ggcgcttcag cgtcatttcg gctgcggcgc cggccgggcc gcccacctgg agcacggttt
     4801 cggcgggcgg cgcgggctgc ccgccccctc tcttcgccgc cttcgcctcg tccagcttcg
     4861 ccctgaggta gctccagttg agcgccacga ggtactggcc gaggcggaag atcttgttgg
     4921 cgtcgtcgcc gtcgaagttg tcgcgcaggg tcgaggcgcg caggaacgcg ccgacgctct
     4981 cgtgcaggtc ggcggagaga atctccttga tcctcccgat ggccgcctcg tcctcacagc
     5041 accgcgcgaa cagctcctcg gcttccctcc tttgcgcttg gtcggagatg tcggggacga
     5101 ggacgcgcag cgcctccggg ctgaggcgcg agacgccctc gtcgaacacc gccttgatct
     5161 gcgcgcgccg ctcgccctcg tccgtcagcc gcgtgtcgag gaaggaggcg gggctgagca
     5221 gaatctgcgg gctgcgcgac tcttgtcgga accggcggac gccgacgttc gcgtagacga
     5281 tgtcgatggc gcggctcagc tcgccgagcg cggtgaagtc gctctgctcg gcgaagagcg
     5341 cgctcgcgtc cgaggagacg gcgacatcca ccttccagtc ttcgagcttg gtcggggcga
     5401 aaagcagcat cgtaagcgcc gagttgtcgg tgatgccgcc gtcggccagc agcaccggcc
     5461 tcttcttctg cgcgttgccg cccggcctgt cgaggtacat ctcttcggag acggcgttga
     5521 aggctccggg gaacgcgccg gaagccgcga ccagccgcga caggtactcg cggcgcggaa
     5581 actcctcgtc cgagaccgcg aagaagaccg acttctcctc gtcgcgcggg gccttgacgt
     5641 tgataaaatc gtccttctcc ttcgcctgaa tcatgaagcg gcgcagcacg ccctcctcgc
     5701 tgaagccgag gaacgagccg ctgtagaggt cggtcgtgca gatcatcagc cgcggcgcac
     5761 ccgccccgcg cttgaggtcg gagagcttct tctggtcgag cagcacgctg tcgagcagcg
     5821 ccgactgcac gtcggagcgc gtgaagctgt accacttgaa gagcttgacg tccgtccacg
     5881 ggagcttgaa cgggaaggga agacgcaggg cgttctgcgc gtcggtcata ttgcgcgtca
     5941 ggttgaagcg gcgctcgttg atggcggcgc gcagccggcg cggctcgccg cccgccgcgt
     6001 agaacgcgcc cgtaatcgag ccgcccgaca ccgaggtcat gtgtgtgacg gggacgccga
     6061 gcgtctcgaa ggcgtcgagc acgcccgcgt gcatcagcac ggcgcggtag ccgccgcccg
     6121 acagcgccac cccgacgcgc acgtcccgcc acggcgactt ctcgtgcagg gcggcaaacc
     6181 gcgccgggtc ggtcggcacc ggcgcgccga cgttccacgc gttcagcccg gagctggtga
     6241 gccagaacag gaagatgaag gagacgaaga agatgaaccc ccaggcccct tgcagcggcc
     6301 tgtgcttcgt aaagtttggt atgacggggc ggccagtgcc gaccgactcc tccgcgcact
     6361 ctttgatgac cgcgtacaga gatttcagcc acagccgccc gcgcgggagc agtaacatca
     6421 cggcgccgaa gctgaagccc atgcagagca ggtcgcgcgt ccggtgatag ctcccggccc
     6481 aagccagcca cgcgaaggag agcagcaggc cgagggcgac ccacgtgccg atgggccgga
     6541 cgtactgctt ccacgcgttg acgacaaacg cgccgaagat gacggcccac gcgataaact
     6601 tcagcgccac catccagcct ttctggctcc ccgtgcagat gaaggcccag gcggaggtga
     6661 cgaggagcac gaagatcacg ccgaagatga tcttgaccag cagttccgcg ccctccagct
     6721 cgcgcctggc cggcgcggcc tcctcctccg cgggcgcggc ggcggggtct cggtcggggt
     6781 tcggggcggg gtcttgcaaa gacatgactc ttgtgaacct cctccgcctc aagcggcggg
     6841 tgtgtgcggt ggtggccgtg ggttcgctga cggccccggc gtgcgcgggg gccgtcagcg
     6901 gggaacggat ggcttagaaa gaacgtgggc tggggtcgtt tctgtgaggc taacggaaag
     6961 gcgtttcgtt agctcggacc cgttggcggt cgtggtggcc ggggcctgcg cgaggccgtg
     7021 tcgcgaacgt ggcgaagcct gtaaccggcg cggcgaaatg tcaacgattt tgcgcgcccg
     7081 acggacgcgg gccggactcg accgcgaacc cgcacggcaa ggtcgagtaa cgggtcggtt
     7141 tcaaacctga caagaccgct ctgaaaccgc ttcgcccgag gcactcgccc cgaataccgc
     7201 gcctacaaat ttatgcgatt tcgcggtgag acgtttcgtc aggccgcacg ggcaccgctg
     7261 aaaagtatag ttcgtgggtt caaactgacc cgctccccga gactaacaag gggccgcccg
     7321 gcgccgggcg gccccttctc acctggcgcg cgcgacaaaa cttcgcgccc gccgcgccgc
     7381 tacagcgcgc ggaggaaggc gacgaggtcg gacttctcgg tctgcgcgag gttcagctcg
     7441 aggacgaggt tgaagaactc gaccgtgtct tcgagcgtga gcaggcgacc gtcgtgcagg
     7501 tagggcggcg actccttgat gccgcgcagg gggaaggtct tgatcggccc ctgcttggta
     7561 atcatcatgc cgccctccat gcgcggcttg aagaagcgct cgacctgtag gtcgtgcatg
     7621 ttgccgtcgg tgtagaaggg cggcgtgtgg cacgtcgcgc agttggcctt gccgaagaac
     7681 agctcctcgc cgcgaacctc ggcggggctg gccttgcggc ggtcgagccg cccgtagacg
     7741 ttcagcttcg gcgcgggcgg gaagtccagc agctcctgga actcggccat cgcgtggacg
     7801 tgcaggccgc ggtcgagcac gttcacgccc ttcttggccg ccgtcaccgg gtcgccgtcg
     7861 aagtaggcgg cgcgctgctc gaactcggtg aagtcttcga ccgtcttgag cgcgcgctgc
     7921 gagccgaaga gccgctggac gttgacgccg cgcaaggaag gcgtctcgat gcggcggcgg
     7981 ttctcctgcg ggcggatgtc gccgacgagg tgcgccgcgc cgagcgtgtg gccgttgacg
     8041 tgacaatcaa aacaagtgac gccgagcatc ccatccgccc gctcggtctt gcggtcggcg
     8101 gtcgcgttga actgctgctg cgggaactgc gtcacgagca gccgcagccc gtcgagctgc
     8161 ttcgggttga ggatgccgtt gaacagctcg tagaagttct cgaccgtgac gagctgcccc
     8221 ttcgacacgt cgccgaggtc ggggcgcgtc gtcaggaaga tggcgggcg
//
LOCUS       DBNGOH010001121         1893 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1454027, whole genome shotgun sequence.
ACCESSION   DBNGOH010001121 DBNGOH010000000
VERSION     DBNGOH010001121.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1893)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1893)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1893
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1454027"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..849)
                     /locus_tag="AC159P_21835"
     CDS             complement(<1..849)
                     /locus_tag="AC159P_21835"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR3698141.1"
                     /translation="MKARVVWLLLCAVWGSTWLFIKLGLRDLPPVSFAGIRFVLAFLI
                     LAGIIAARGTRLPRAASDYALIALTGVLAFSINYGLLFWGEQHISSGLAALLQATIPA
                     FGLALAHLHLPGERLTAAKAAGVVLGIAGVGVIFSNQLAVAGRMALWGSAAIVAGAAC
                     AAYSNVLVKARGREIDPAVLAAGQMLCGLVPLVAAGAAFEGNPLGFRWTRLAVVSLLY
                     LTIVGSVIAFMLYYWLVRNMDVTKTMLIALVTPVVAVLLGMAVLGEELTWRTLAGAAL
                     IMSGIAL"
     gene            complement(1106..1498)
                     /locus_tag="AC159P_21840"
     CDS             complement(1106..1498)
                     /locus_tag="AC159P_21840"
                     /inference="COORDINATES: protein motif:HMM:NF014950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR3698142.1"
                     /translation="MFENAKQYAPQYALQGDAAPEGAQDQATSALKLKTLKELTLALL
                     KEVDSLKETQAPEGGKTNVNFSEEVRRFETELIRWALLRTGGHQRRAARLLNIKVTTL
                     NAKIKRYGIRPASLPAGNVVDFHPENRQ"
     gene            complement(1586..>1893)
                     /locus_tag="AC159P_21845"
     CDS             complement(1586..>1893)
                     /locus_tag="AC159P_21845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3698143.1"
                     /translation="TYKNHQTGATYRLFKEATVNSDGPLFERNALLAERKQSRVGSFL
                     STSRQARVLMFLHELGHVVAREDGKWVLPNDGDDYLQSERNTRKVEEVCSEQLAAIE"
BASE COUNT          305 a          691 c          601 g          296 t
ORIGIN      
        1 gagcgcgatg cccgacatga tgagcgcggc gcccgcgagc gtgcgccacg tcagctcttc
       61 gccgagcacg gccatcccca gcagcaccgc gacgaccggc gtgacgagcg cgatcagcat
      121 cgtcttcgtc acgtccatgt tccgcaccag ccagtagtag agcatgaagg cgatgaccga
      181 gccgacgatg gtcaggtaca gcagcgacac gacggcgagc cgcgtccagc ggaacccgag
      241 cgggttgccc tcgaacgcgg cgcccgccgc gacgagcggc accagcccgc acaacatctg
      301 ccccgcggcc agcaccgccg ggtcgatctc gcgcccgcgc gccttgacga gcacgttcga
      361 gtaggccgcg cacgccgcgc ccgcgactat cgccgcgctc ccccacaggg ccatccgccc
      421 cgccaccgcc aactggttcg agaagatcac gccgacgccc gcgatgccta agacgacgcc
      481 cgccgccttc gccgccgtca gccgctcgcc cggcaggtgc aggtgcgcga gcgccagccc
      541 gaacgcgggg atcgtcgcct gcaacagcgc cgctagcccc gaagagatgt gctgctcgcc
      601 ccagaacagc agcccgtagt tgatcgagaa cgcgagcacg cccgtcaacg cgatgagcgc
      661 gtagtcggag gcggcgcgcg gcagccgcgt cccccgtgcc gcgatgatgc ccgcgaggat
      721 gaggaacgcg agcacgaagc ggatgcccgc gaagctcacg ggcggcaagt ctcgcaaccc
      781 gagcttgatg aacagccacg tggaacccca cacggcgcat agcagcaacc agacgacgcg
      841 agccttcatc gaccagaatc tataagccac gcgcgcgagc gaggtcaacg cctcagcggc
      901 gcaaaaaaaa atgtagcccg cccgcgggct acattccgcc tgccccgtcc gaggggcggc
      961 gctttcacac tctcgcggca ggcgcttcag ccgcggcggc gcgcggcgcg cgtcacctcg
     1021 cccccttgct ctttgccgac tgccgaccga ctgctgtcta ccgactgact gccgactgct
     1081 gactgctcac cgccgaacgc ttacttcact gccggttctc cgggtggaag tccacgacgt
     1141 tgccggcggg cagcgaggcg gggcggatgc cgtagcgctt gatcttggcg ttgagcgtcg
     1201 tcaccttgat gttgagcagc cgcgcggcgc ggcgctggtg gccgcccgtg cgcaggagcg
     1261 cccagcggat cagttcggtc tcgaaccgcc gcacctcttc cgagaagttg acgttggtct
     1321 tgccgccctc gggcgcctgc gtctccttca acgaatcgac ctccttcagc agggcgagcg
     1381 tcagctcttt cagcgtcttg agcttgagcg ccgaggtggc ctgatcctgc gcgccctccg
     1441 gggctgcgtc cccctggagc gcgtactggg gtgcatactg tttcgcgttc tcgaacatac
     1501 cgggaaaact cctttcgtcc cctcctgcgt ttgatctgag ccggcgacgg cgcgcgctcg
     1561 ggaaggcgcc gcgcccgccg ggttgttatt cgatggccgc gagctgctcg gagcagacct
     1621 cctcgacctt cctcgtgttc cgctcgctct gcaagtagtc gtcgccgtcg ttcgggagca
     1681 cccacttgcc gtcctcgcgc gccaccacgt ggccgagttc gtggagaaac atcagcaccc
     1741 tggcctgcct cgacgtggac aggaagctgc cgacccgcga ctgcttcctc tcggccagca
     1801 gcgcgttccg ttcgaagagc ggcccgtcgc tgttgaccgt cgcctccttg aagagcctgt
     1861 aggtggcgcc ggtctggtgg ttcttgtagg tca
//
LOCUS       DBNGOH010001122         6602 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_719431, whole genome shotgun sequence.
ACCESSION   DBNGOH010001122 DBNGOH010000000
VERSION     DBNGOH010001122.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6602)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6602)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6602
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_719431"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            13..2412
                     /locus_tag="AC159P_21850"
     CDS             13..2412
                     /locus_tag="AC159P_21850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HIR3698144.1"
                     /translation="MFERYTEKARRVIFFARYEASQFGAPAIEPEHLLLGLMREDKTL
                     TSRFLARAQASLEAIRKEIEGRAPLREKISTSVELPLAPETKRVLAFAHEESDRLQHR
                     HIGTEHLLLGLLREERSMAAEILYERGLRLPAVREEVARASGIDPRAAQKKETPHLAE
                     FSRDLTDDAANDKLDPLVGREGEIERVVQILCRRTKNNPVLIGEPGVGKTAIVEGLAQ
                     RIVRGEVPSFLENKRILSLDLSLIVAGTKYRGQFEERLKQIMRELVENPQYIVFIDEL
                     HTLVGAGSAEGSLDAANILKPALSRGEIQCIGATTPAEFRKSIEKDRSLERRFQAVKV
                     PPPNEEEAVRILDGVRERYEAFHQVRYTDEAIEAAVYQSNRYIPDRFLPDKAIDVIDE
                     AGARVKLRARRDSMSGAAARGDMPRNEVFDDRDRSIYHRSPTFVNSPLTEEDEMLLSV
                     EVTRDDIEEVIARWTGIPVMSLKEEETRKLLRIEAELHGRVIAQRPAISALARSIRRS
                     RAGLKNPHRPVGSFLFLGPTGVGKTEVARSLAEFLFGSERSRIRFDMSEFMEKHSVSK
                     LIGSPPGYVGHEEGGQLTERIKRSPYSVLLFDEIEKAHPDIFNVLLQVFEDGILTDAL
                     GNTVDFKNAIIIMTSNIGARFIQKKGTMGFQSTPDSSREKLEEMVMSAVRQTFNPEFI
                     NRLDEIIIFDQLVDDELLEIVGLQVEQMNKTLSRRGLEVRLTDEAKRWIVEKTCGDRS
                     YGARPLRRALQKYVEDPLSEALIQGTLGDGPLIEVYLDGDFLSHRPAGVEEPGDALLI
                     H"
     gene            2768..5863
                     /gene="bamA"
                     /locus_tag="AC159P_21855"
     CDS             2768..5863
                     /gene="bamA"
                     /locus_tag="AC159P_21855"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0071709 - membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HIR3698145.1"
                     /translation="MRTRATWLFLVVAALAGCVTLFDLAPPSTRARAQEPATQQKLVE
                     DVLIEGNRRLRDEDILYHIQTRQGDPYNEDQVQRDLQALLNLPFFDKTDTRVAREDGP
                     RGGVRVIFYVRELPIIRDLTFKGLGSIGEADVLKAFREQRAGVSKETAYDPVKVNSSK
                     RIIRELLAQKGHPNATIDAQVEEISQTSVAITFDVDEGDRVRVVEIDFEGNQVFSDGE
                     LRGAMKLVKEAGLISRFKGEDILHTEKLEYDLKANVVNHMRSKGYLEARTLDPRIEGI
                     GERRTGFPVLPLPLLSSKDDALRVTVPIIEGKLYRLGEIKIEGNSIFSEQDIRAVIGL
                     QKGDVANGFRLNKALNEDIKKLYGRAGFIQYFAEFEPEFKPSPQNPQEGIADFTITIT
                     EGKQFSLRRLEFLGNTFTRDNVLRREVALNEGDIFDETLWEFSVLRLNQLGFFDPIEK
                     EKDAEFRTDEEIGTVDINLRVSERGRNQIAFNGGLSGIGGSFFGLEYSTNNLLGRGES
                     LSFQFAVGNRQRSFLFSFTEPYVRDRPVSVGFSLFAESRKFFGEGTFLSQNIDAITGT
                     IDPIAFQTVGDENLFTQNTAGISLFASSPLSEFYRPRSRRMTEIVRATRIGLSYSISQ
                     SSIEDPPINEQNDPNTFIPRVFTQPNILTSRLTPSLVYDTRNGSIDPTSGRQLSAQFA
                     FAGLGGDVRTYQPTISYMQFIPVRRKRSENPEVFGFRLIAGHVGSFGITPTVEAAQRS
                     SLSFLNGVPIYERFFVGDEFSIRGYDVRSIGPIIPLEVFITSRDVAVSATGAGDIEPL
                     PGLSERLNTQLETLGTFTGSSGANSLLLSRTFRLIGGDTQLLGNFEYRVPIFGPLQLA
                     AFADIGTAFNLRGGADQEFSTDFLPDNTFLPLQGFTGLAQLVALKNPQLQFVPTTGSL
                     VIRGDRLVTKEEFVNAQRGPTDPFTGLPIGMQAVFLRAAPTTLTPDGGVQTNTVARLS
                     QAQLSKIGDYRSSVGLEARIQLPVVNVPFRLIYAFNPNARTEFIREKRSLFRFSIGRT
                     F"
     gene            5962..6582
                     /locus_tag="AC159P_21860"
     CDS             5962..6582
                     /locus_tag="AC159P_21860"
                     /inference="COORDINATES: protein motif:HMM:NF015871.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH/Skp family outer membrane protein"
                     /protein_id="HIR3698146.1"
                     /translation="MKIFRTIFAATLMTAAAALAVNAQPAATRPAATPPAAPAASAQL
                     GEAKIALIDTGAFADEKQGIARMIAAVKRVETQFQTVRTELQGMRANLERLTKEINDT
                     KGVADQAALARKAEQAETLEKEIKRKGEDAQGNYQKAMRDALQPVQQDILKALETYAK
                     GRGITVIIDAAQVPILYATPNMDITAAFIADYNQRNPASAAAAAPR"
BASE COUNT         1221 a         2391 c         1972 g         1018 t
ORIGIN      
        1 gtcacagctt ttatgttcga acgctacacg gagaaagcgc ggagggtgat tttcttcgcg
       61 cgctacgagg ccagccagtt cggggcgccc gccatcgaac ccgaacacct cctgctcggc
      121 ctgatgcgcg aagacaagac gctgacctca aggttcctgg cgcgcgcgca ggcgagcctc
      181 gaagcgatcc gcaaggagat cgaggggcgc gccccgctgc gcgagaaaat ctccacgtcg
      241 gtcgagctgc cgctcgcgcc cgagaccaag cgcgtgctcg ccttcgcgca cgaagaatcc
      301 gaccgcctcc agcaccgcca catcggcacc gagcacctgc tgctcggcct cctgcgcgaa
      361 gagcgctcga tggccgcgga gattctctac gagcgcggcc tgcgcctgcc ggcggtgcgc
      421 gaggaagtcg cgcgcgcctc gggcatcgac ccgcgcgccg cgcagaagaa ggagacgccg
      481 cacctcgcag agttctcgcg cgacctgacg gacgacgccg cgaacgacaa gctcgacccg
      541 cttgtgggcc gagagggcga gatcgagcgc gtcgtccaaa tcctctgccg ccgcacgaag
      601 aacaaccccg tcctgatcgg cgagccgggc gtcggcaaga ccgccatcgt cgaaggtctc
      661 gcgcagcgca tcgtgcgcgg cgaggtgccc tcgttcctcg aaaacaagcg catcctctcg
      721 ctcgacctgt cgctcatcgt cgcgggcacc aagtaccgcg ggcagttcga agagcgcctg
      781 aagcagatca tgcgcgagct ggtcgagaac ccgcagtaca tcgtcttcat cgacgagctg
      841 cacacgctgg tcggcgcagg ctccgccgaa ggctcactcg acgcggcgaa catcctgaag
      901 ccggctctgt cgcgcggcga gatacagtgc atcggcgcga ccaccccggc ggagttccgc
      961 aagtccatcg agaaagaccg ctcgctcgaa cgccgctttc aggccgtcaa agtccccccg
     1021 ccgaacgagg aggaggccgt gcgcatcctc gacggcgtgc gcgagcgcta cgaagccttc
     1081 caccaggtgc gctacacgga cgaagccatc gaggccgcgg tctatcagtc gaaccgctac
     1141 atccccgacc gcttcctgcc ggacaaggct atcgacgtga tcgacgaggc gggggcgcgc
     1201 gtcaagctgc gcgcgcggcg cgactcgatg agcggcgcgg cggcccgcgg cgacatgccg
     1261 cgcaacgagg tgttcgacga ccgcgaccgg agcatctacc accgcagccc gactttcgtt
     1321 aactcgccct tgaccgaaga ggacgaaatg ctcctctcgg tcgaggtcac gcgcgacgac
     1381 atcgaggagg tcatcgcgcg ctggacgggc atccccgtca tgtccctgaa ggaggaagag
     1441 acgcgcaagc tgctgcgcat cgaggcggag ctgcacgggc gcgtcatcgc gcagcgaccg
     1501 gccatctcgg cgctcgcgcg ctcgatccgc cgctcgcgcg cgggcctcaa gaacccgcac
     1561 cggcccgtcg gctcgttcct cttcttaggc ccgaccggcg tcggcaagac ggaggtcgcg
     1621 cgcagcttgg ccgaattcct cttcggctcc gagcgctcga ggatccgctt cgacatgagt
     1681 gagttcatgg agaagcactc ggtctcgaag ctcatcggct cgccccccgg ctacgtcggc
     1741 cacgaggagg gcggccaact gaccgagcgc atcaagcgct cgccctactc ggtcctcctc
     1801 ttcgacgaga tcgagaaggc gcacccggac atcttcaacg tgctgctgca agtcttcgag
     1861 gacggcatcc tgacggacgc gctgggcaac acggtcgatt tcaagaacgc catcatcatc
     1921 atgacctcga acatcggcgc gcgcttcatc cagaagaagg gtacgatggg cttccagtcc
     1981 acgcccgact cgtcgcgcga gaagctggaa gagatggtga tgagcgccgt ccgccagacc
     2041 ttcaaccccg agttcatcaa ccgcctcgac gagatcatca tcttcgacca gttggtggac
     2101 gacgagcttt tggagatcgt cggtctccag gtcgagcaga tgaacaagac gctctcgcgc
     2161 cgcggcctcg aagtgcggct cacggacgag gccaagcgct ggatcgtcga gaagacctgc
     2221 ggcgaccgct cctacggcgc ccggcccctc cggcgcgcgc tgcaaaagta cgtcgaagac
     2281 ccgctctccg aggcgctcat ccagggcacg ctcggcgacg gcccgcttat cgaggtctat
     2341 ctggacggcg acttcctcag ccaccgcccg gcgggcgtcg aagagccggg cgacgcgctg
     2401 ctgattcact aaagtgaatc ggggaaaaag gggaagaggg aaagggcgaa aagggacata
     2461 aacgctttcc ttttcgccct ttcccctctt ccccttttcg ccctccgtgc ccctttacgc
     2521 cccttcccct tttgcccttt cccccccgtg ccctgtataa tgcgccactt tgctgggaca
     2581 agtgtctgga caaccgcggg cgtgtcgggg tgaatccaaa aggctcacgg cagaatgcgc
     2641 gccctcgccg aagatggccc cgcagggccg gcgtgccgtg tccgactcgg gcccgacaga
     2701 agctctaaag ttttcataaa gtctcccttt cccggctgct atcttcttga ggcacaaagc
     2761 tacagttatg cgaacacgcg cgacctggct cttcctcgtc gtggccgccc tcgcgggttg
     2821 cgtcacgctc ttcgacctcg cgccgccctc cacgagggcg cgcgcgcagg agccggccac
     2881 acagcagaag ctcgtcgagg acgtgctgat cgaaggcaac cgccgcctgc gcgacgaaga
     2941 catcctctac cacatccaga cgcggcaggg cgacccttac aacgaggatc aggtgcagcg
     3001 cgaccttcag gcgctcctca acctcccctt cttcgacaag accgacacgc gcgtggcgcg
     3061 cgaggacggg ccgcgcggcg gcgtgcgcgt catcttctac gtcagggagc tgccgatcat
     3121 ccgcgacctc accttcaagg gcctcggcag catcggcgag gccgacgttt tgaaggcgtt
     3181 ccgcgagcag cgcgcgggcg tctcgaagga gacggcctac gaccccgtca aggtcaacag
     3241 ctcgaagcgc atcatcaggg aactcctcgc gcagaagggg cacccgaacg cgaccatcga
     3301 cgcgcaggtc gaggagattt cgcagacctc cgtcgccatc accttcgacg tggacgaggg
     3361 cgaccgcgtc cgcgtcgtcg agatcgactt cgagggcaat caggtcttct cggacggcga
     3421 gctgcgcggc gcgatgaagc tcgtcaagga ggccggcctc atctcgcgct tcaagggcga
     3481 ggacatcctc cacaccgaga agctcgaata cgacctcaag gcgaacgtcg tcaaccacat
     3541 gcgctcgaag ggctacctcg aagcgcgcac gctcgacccg cggatcgagg ggatcggcga
     3601 gcggcgcacg ggcttccccg tcctgccgct cccgctgctt tcttcgaagg acgatgcgct
     3661 ccgggtcacc gtccccatca tcgagggcaa gctctaccgc ctcggcgaga tcaagatcga
     3721 gggcaactcg atcttctccg agcaggacat ccgcgccgtc atcggcctcc agaagggcga
     3781 cgtggccaac ggcttccgcc tcaacaaggc tctcaacgag gacatcaaga agctctacgg
     3841 gcgcgcgggc ttcatccagt atttcgccga gttcgagccg gaattcaagc cgagtccgca
     3901 gaacccgcag gagggtatcg ccgacttcac catcaccatc accgagggca agcagttcag
     3961 cttgcgccgc ctcgaatttt tgggcaacac cttcacgcgc gacaacgtgc tcaggcgcga
     4021 ggtggccctc aacgagggcg acatcttcga cgagacgctc tgggagttct cggtgctgcg
     4081 cctgaaccag ctcggcttct tcgaccccat cgagaaggag aaagacgccg agttccgcac
     4141 ggacgaggag atcggcacgg ttgacatcaa cctgcgcgtc tccgagcgcg gacgcaacca
     4201 gatcgccttc aacggcggac tgtccggcat cggcggctcg ttcttcgggc tggagtattc
     4261 gaccaacaac ctgctggggc gcggcgaatc gctctcgttc cagttcgccg tcggcaaccg
     4321 ccagcgctcg ttcctcttct cgttcaccga gccttacgtc cgcgaccgcc cggtctcggt
     4381 cggcttctcg ctcttcgccg agtcgcgcaa gttcttcggc gagggcacct tcctctcgca
     4441 gaacatcgac gccatcacgg gcaccatcga ccccatcgcc ttccagaccg tcggcgacga
     4501 gaacctgttc acgcagaaca cggcgggcat ctcgctcttc gccagctcgc ccctctccga
     4561 gttctaccgg ccccgcagcc ggcgcatgac cgagatcgtg cgcgccacgc gcatcggcct
     4621 ttcgtactcg atctcgcagt cgtccatcga agacccgccg atcaacgagc agaacgaccc
     4681 gaacacgttc atcccgcgcg tcttcacgca gcccaacatc ctgaccagcc gcctgacgcc
     4741 ctcgctcgtc tacgacacgc gcaacggctc catcgacccg accagcgggc ggcagctctc
     4801 ggcgcagttc gccttcgccg gcctcggcgg cgacgtgcgc acctaccagc cgaccatcag
     4861 ctacatgcag ttcatccccg tccggcgcaa gcgttcggag aacccggagg tcttcggctt
     4921 ccgcctcatc gcgggccacg tcggcagctt cggcatcacg ccgacggtcg aggcggcgca
     4981 gcgctcctcg ctctcgttcc tgaacggcgt cccgatctac gagcgcttct tcgtcggcga
     5041 cgagttctcg atccgcggct acgacgtgcg ctccatcggc ccgatcatcc cgctcgaagt
     5101 cttcatcacc tcgcgcgacg tggccgtctc ggcgaccggc gcgggcgaca tcgagccgct
     5161 gcccggcctc tccgagcgcc tgaacacgca gctcgaaacg ctcggcacct tcaccggctc
     5221 gtccggcgcc aactcgctcc tgctctcgcg caccttccgc ctcatcggcg gcgacacgca
     5281 gctcctcggc aacttcgagt accgggtgcc gatcttcggc ccgctccagc tcgcggcctt
     5341 cgccgacatc ggcacggcct tcaacctgcg gggcggcgcc gaccaggagt tctcgaccga
     5401 cttcttgccc gacaacacgt tcctcccgct gcaaggcttc accggcctgg cgcagctcgt
     5461 cgcgctcaag aacccgcagc tccagttcgt cccgaccacc gggagcctcg tcatccgcgg
     5521 cgaccgcctc gtcacgaagg aggagttcgt caacgcgcag cgcggcccca ccgatccgtt
     5581 cacgggcctg cccatcggca tgcaagccgt cttcctgcgc gccgcgccca cgaccctcac
     5641 gcccgacggg ggcgtgcaga ccaacaccgt cgcgcggctc tcgcaggcgc agttgtcaaa
     5701 aataggcgac taccgatcca gcgtcggact ggaagcgcgc atccaacttc ccgtcgtcaa
     5761 cgtgccgttc cgtttgatct acgccttcaa cccgaacgcg cgcaccgaat tcatccgcga
     5821 gaagagaagc ctgttccgct tcagcatcgg gcggacattc tgaccgtttg gagttaaaat
     5881 gcgggggccg tcgccccttc gaccggaggc gagcaatcgt ccccctttac cagactgtta
     5941 aggagcagta caagaaaaga gatgaagata ttccgcacca tctttgccgc cacgctgatg
     6001 acggcagccg ccgccctcgc cgtcaacgcc cagcccgccg cgacccggcc cgccgccacg
     6061 ccgcccgccg cgcccgccgc gagcgcgcag ctcggcgagg ccaagatcgc gctcatcgac
     6121 accggcgcct tcgccgacga gaagcagggc atcgcccgca tgatcgccgc cgtcaagcgc
     6181 gtcgagacgc agttccagac cgtccgcacc gagctgcaag ggatgcgggc caacctggag
     6241 cggttgacca aggagatcaa cgacacgaag ggcgtcgccg accaggccgc gctcgcgcgc
     6301 aaggccgagc aggccgagac gctcgagaag gagatcaagc gcaagggcga ggacgcgcag
     6361 ggcaactacc agaaggccat gcgcgacgcg ctccagcccg tccagcagga catcctcaag
     6421 gcgctcgaaa cctacgccaa gggccgcggc atcaccgtca tcatcgacgc cgcgcaggtg
     6481 ccgatcctgt acgccacgcc gaacatggac atcaccgccg ccttcatcgc cgactacaac
     6541 cagcgcaacc cggcgtccgc cgccgccgcc gccccgcgct aaacgcggaa agaagtgctg
     6601 ag
//
LOCUS       DBNGOH010001123         7438 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2738012, whole genome shotgun sequence.
ACCESSION   DBNGOH010001123 DBNGOH010000000
VERSION     DBNGOH010001123.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7438)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7438)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7438
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2738012"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            823..4284
                     /locus_tag="AC159P_21865"
     CDS             823..4284
                     /locus_tag="AC159P_21865"
                     /inference="COORDINATES: protein motif:HMM:NF012356.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pullulanase X25 domain-containing protein"
                     /protein_id="HIR3698147.1"
                     /translation="MSFPKHFRLAASLTTLGLAVVTLLTTFMFPATLASHTPNPSSVT
                     VAGSLQSELGCSGDWQPECAATHLVYDANDDVWQQSFDVPAGNWEYKAPVNNSWDENY
                     GAGGVQNGANIPLNLGAGAAVKFYYDHKSHWITSNRNSVIAVAPGSFQSELGCSGDWD
                     PSCLRSWLQDLDGDGTYTFRTTSLPAGNYEAKVAINESWDENYGAGGVPGGANIGFNV
                     PTNNAPVTFSYNSTSHVLTINTAAPAGDVQWDGLRHDTFDSYYRSPFGAVPAGTAVTL
                     RFRTAHFDVTGVHVRVYTFDPATGQTAGPVDHAMSFLENRTEAGTEYDIWTHTLETPA
                     APAILYYKFRVTDQADEDFYSDAYADDHDNLNQGGAGAASDGEPFPAFQLTAYDPNFQ
                     TPAWLHDANVYQIFPDRFRNGDPSNDYCRPGSTAGCPAFYGDQTPLLREPWNTLIGDP
                     RQPGPFRNEYGTQFYGGDLKGIENRLDYLQTVGVDTLYLNPIFKARSNHRYDTDDFLE
                     VDPALGGDAALVSLKTEMERRGMRLILDGVFNHSSSDSVYFDLYHRYDAPDGGCENAA
                     SPYRGWFNFFNSNAPCSFGDYEAWFGFGSLPVFKDDSGAVRDFFYRTPGANVTEYWYE
                     RGAAGWRFDVANEISHDWWNEYRPLAKSYKADGPLVGEIWPDASQWLAGDQLDSVMNY
                     RFRKNVLGFARGQFDWGDNDNSGGNRIVALNPSQFDRALRSVREDYPAQATAAMLNLI
                     DSHDTNRSLYVLTVGGDNGLAQAKERLKLSALFQFTYMGAPMVYYGDEAALDAPSLAN
                     GPNGPEDDPYNRAPYPWADEAGDQDAYGPADQSVVAYYTTLAHMRKQHASLRTGSFET
                     LLTGDTTPSTTDDNTYAFARAGGGPTAIVILNNGTLDNTASVPVAGHFADGATLRDAL
                     GGATYNVSGGHVNLTLAPRSGVVLFDSAVAPVDTTAPVATIGVNPPANAGGWNNTAPV
                     TVNLSATDGGSGIKELRYWVDGGAVNVAAGDAAGIQLDTEGVHTVGVRAIDNAGNISA
                     LVTQVVKLDLTAPMVNGASANPSTLSPPNHKMRDVTVAYSASDNFGPATCSLNVTSNE
                     PVNADGDGDGDTAPDWEILDAHHVRLRAERSDAGTGRVYTVNITCADAADNTTTRALT
                     VFVPRGR"
     gene            4608..7352
                     /locus_tag="AC159P_21870"
     CDS             4608..7352
                     /locus_tag="AC159P_21870"
                     /inference="COORDINATES: protein motif:HMM:NF046269.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HIR3698148.1"
                     /translation="MKTRRLFFGFALCFLFFPPGHLWRGAQAQTPSRLTPKFPLRGSG
                     LELERRTQAGSFFDVAGRKAAVFGYEHRPLEAWVYPLKLLDDFELSFRVEGYPLAFRA
                     SDMAVRVNVRPEATVFTYSHAAFSVRQIIYAPVDEPGVVMLLDVASVLPMTVEVSFRP
                     QLKLAWPAGLMTGNIGWVEKQHVYYITEESRRFVGVVGSPAGRDVSVMPYQEEPRDVP
                     SRFLIETSPAELKTSFIPIVIAGSVEGREKAKAAYDRLLGSARSLYENNVAYYERLQR
                     ETTSIETPDERFDTAFDWAKVGVDKGIATNPFLGTGLLAGFRTSGQSERPGFAWYFGR
                     DALWTALAINSYGDFRSARTALEFLKKFQRADGKIPHEISQSASLIPWFTDYEYPWAS
                     ADATPLYVIAHADYWRATGDTGFLQTNWDSIRQAYRFSEATDTDGNQLIENTKFGHGW
                     VEGGPLRPVHEEIYMQGLWIEASRSMAELAEALKDGATASRARAAAERTRAAMEQTYW
                     LADRGFYAFGTRLQPTNETPEAEPGPNRAARQARMEELATARLIDEDTVLPAVPLWWH
                     TMLDERAQSQIDHLGGGRMATDWGARIISDESRLYDPLSYHYGSVWPLFTGWASMGAY
                     AYGRPHVGHQALMANALLSYTGALGYVTELLSGDFNAPFGRSSHHQVWSEAMVVTPAL
                     RGLLGIEIDEGGKELRFAPQLPANWDRVAALNVAAGDARYDLKLARAAGRLTITVTQR
                     ISAASGSSSLSRRVERFTFAPAFPLDARVRKVTVQGRATNFEMKSGGDVQRAEVGIEA
                     REPSVEIVFTYTEGTDVYHVPQSLTPGAESEGLRVLRSRADRDALRLLLEGIGGRTYA
                     VRVRTPHQLGPADGVSVQPDDNSDPLLAVAFEGASGSYVRREVTIPLRRAAAARSKRK
                     "
BASE COUNT         1316 a         2609 c         2246 g         1267 t
ORIGIN      
        1 cgcccatgtg tcgaactgcc ccttcctgat cttccgcgct gattctatcc cgctgatcgt
       61 caccgcccca ttcccgaagc tcttgaaccc cggcatccga tggaggcgct gcttcactct
      121 cctgtagctc ccgcccgccg aggtcgttca agtattcact catccagacc ttcgtccgcg
      181 gacgcggcct tccactattc tcgagtcccg ccacggcaga ctgagtggcg ggacagccgt
      241 cgagcgctat tctttcgggg gcgccgtgtc gctcaatcgc tccggtaaag actgcttggc
      301 cgctgctaca tctccatgct cgctcgatag gaaatcagcc gtcagtccct gcttatcggc
      361 gagcgcgata gaggtacgtc cggcgccctc cggctccgat gtccgttccg tccactcgcc
      421 aggaggtgcc gacgggtcgt gtgtagcggc cccatcgttt ctcgaactcg ggggcgtagt
      481 gttggaccca tccgaggatt gtcgtatgcg agacagagac accgcgctcg gccatcagct
      541 cgacggctcc ggtgcaaata gcatgagtgc atcacggata cttcgttaag tccgacatcg
      601 agcggcaagt acttcgcgtt gacggcgtga agcttgacgg ttcgtgccga ttaaagtaag
      661 ttgtcgcgtc gcacaaagtc gggctggctg ccattatccg cgttcgccgc gatcttcctt
      721 ccagcctttt tttcaagcgt cgaaagccca aacatctctc acgtcatcgt ggccgccaag
      781 cgtcttcgcc ggtcgcctgt ttccaattcg aaggagtgcg tcatgtcgtt ccctaaacac
      841 ttccgcctcg cggcgtccct cacgactctc ggtctggctg ttgtcacgct gctgaccacc
      901 ttcatgttcc ccgcgactct ggcgtcgcac acgccgaacc cgtcgagcgt caccgtcgcc
      961 ggcagtcttc agagcgaact cggctgctcc ggcgactggc agccggagtg cgccgcgaca
     1021 cacctcgtct acgacgcgaa cgacgacgtg tggcagcaga gcttcgacgt ccccgcgggc
     1081 aactgggagt acaaggcgcc ggtcaacaat agttgggatg aaaactacgg cgcgggcggc
     1141 gtgcagaacg gggcgaacat ccccttgaat ctcggcgcgg gcgcggcggt caaattctat
     1201 tacgaccata aatcacattg gattaccagc aaccgcaatt ccgtcatcgc cgtcgcgccc
     1261 ggcagcttcc agtccgaact cggctgctcc ggcgattggg acccgagttg tctgcgctcc
     1321 tggctgcaag acttggacgg cgacggcacg tacacctttc gaactacttc gctgccggca
     1381 gggaactacg aagcgaaggt cgccatcaac gaaagctggg acgagaacta cggcgcgggc
     1441 ggcgtgcccg gcggggcgaa catcgggttc aacgtgccga ccaacaacgc gcccgtgacc
     1501 ttcagctata actcgacgag ccacgtcctg acgatcaaca ccgccgcgcc cgcgggcgac
     1561 gttcaatggg acggcctgcg ccacgacacg tttgatagtt attaccgcag ccccttcggc
     1621 gccgtgcccg cgggcaccgc tgtcacgctc cgcttccgca ccgcgcactt cgacgtgacg
     1681 ggcgtccacg tccgggtcta caccttcgac cccgcgaccg gccagaccgc cggccccgtc
     1741 gatcacgcga tgtccttcct cgagaaccgc accgaagcgg gcaccgagta cgacatctgg
     1801 acgcacacgc ttgagacgcc cgccgcgccc gcgattctct attacaagtt ccgcgtcacc
     1861 gatcaggccg acgaggattt ctacagcgac gcttacgcgg acgaccacga caacctgaat
     1921 cagggcggcg cgggcgcggc ctcggacggc gagccgttcc cggccttcca gctcacggcc
     1981 tacgacccga acttccagac gcccgcgtgg ctccacgacg cgaacgtcta ccaaatcttc
     2041 cccgaccgct tccgcaacgg cgacccttcg aacgactact gccgccccgg ctcgaccgcc
     2101 ggctgccccg cgttctacgg cgaccagacg ccgctcctgc gcgagccttg gaacacactc
     2161 atcggcgacc cgcgccagcc cggcccgttc cggaacgagt acggcacgca gttctacggc
     2221 ggcgacctca aaggcatcga gaaccgtctc gactacttgc aaacggtcgg cgttgacacg
     2281 ctctacctca accccatctt caaggcgcgt tcgaatcatc gttacgacac ggacgacttc
     2341 ctcgaagtcg atccggcgct cggcggcgac gccgcgctcg tctcgctcaa gaccgagatg
     2401 gaacggcgcg gcatgcgcct catcctcgac ggcgtcttca accactcgtc ttccgacagc
     2461 gtctacttcg acctctatca tcgctacgac gcgcccgacg gcggatgcga gaacgcggcg
     2521 tcgccgtacc gcggctggtt caacttcttc aactcgaacg cgccctgctc gttcggcgac
     2581 tacgaggcgt ggttcggctt cggcagcctg cccgtcttca aagacgactc gggcgcggtg
     2641 cgcgacttct tctaccgcac gcccggcgcg aacgtgaccg agtactggta cgagcgcggc
     2701 gccgcgggct ggcgcttcga cgtggcgaac gaaatctcgc acgactggtg gaacgagtac
     2761 cgcccgctgg cgaagagcta caaagcggac gggccgctcg tcggcgaaat ctggcccgac
     2821 gccagccagt ggctcgcggg cgatcaactc gactcggtga tgaactaccg cttccgcaag
     2881 aacgtgctcg gcttcgcgcg cgggcagttc gactggggcg acaacgacaa cagcggcggc
     2941 aaccggatcg tcgcgcttaa ccccagccag ttcgaccgcg cgctccgctc cgtgcgcgag
     3001 gactacccgg cgcaggcgac cgccgcgatg ctcaacctca tcgactcgca cgacaccaac
     3061 cgctcgctct acgtcttgac cgtgggcggc gacaacggac tcgcgcaggc caaagaacgt
     3121 ctcaagctct ccgcgctctt ccagttcact tacatgggcg cgccgatggt ctactacggc
     3181 gacgaagcgg cgctcgacgc gccctcgctc gccaacggcc ccaacggccc cgaggacgac
     3241 ccgtacaacc gcgcgcccta cccgtgggcc gacgaagcgg gcgatcagga cgcctacggc
     3301 cccgcagacc aatcggtcgt cgcctactac acgacgctcg cgcacatgcg caagcagcac
     3361 gcgtcgctcc gcacaggctc cttcgagacg ctgctcaccg gcgacacgac cccttcgaca
     3421 accgacgaca acacctacgc cttcgcgcgc gcgggcggag gcccgaccgc catcgtcatt
     3481 ctcaacaacg gcactctcga caacacggcg agcgtccccg tcgccggtca cttcgccgac
     3541 ggcgccacgc tccgcgacgc gctcggcggc gcgacctaca acgtcagcgg cggccacgtg
     3601 aatctcacgc tcgcgccccg cagcggcgtc gtgctcttcg attcggcggt cgcgcccgtg
     3661 gacacgaccg cccccgtcgc aaccatcggc gtcaacccgc ccgccaacgc cggaggctgg
     3721 aacaacaccg cccccgtcac cgtcaacctg agcgcgaccg acggcggcag cggcatcaag
     3781 gagttgcgct actgggttga cggcggcgcc gtcaacgtcg ccgcaggcga cgctgccggc
     3841 atccagctcg acaccgaagg cgtccacacc gtcggcgtcc gcgccatcga caacgcgggg
     3901 aacatcagcg ccctcgtcac gcaggtcgtc aaactcgacc tcacggcccc gatggtcaac
     3961 ggcgcctccg cgaacccttc aaccctgtcg ccgcccaatc acaagatgcg cgacgtgacc
     4021 gtcgcttaca gcgcttcgga caacttcggc cccgcgacct gttcactcaa cgtcacgagc
     4081 aacgagccgg tcaacgccga cggcgacggc gacggcgaca ccgctcccga ctgggaaatt
     4141 ctcgacgcgc atcacgtccg cctccgggcg gagcgttcgg acgcgggcac gggccgcgtc
     4201 tacaccgtca acattacctg cgcggacgcc gcggacaaca cgacgacgcg cgcgttgacg
     4261 gtctttgtcc cgcgcggtcg ttagacgttc ctgacggcgt tcgaaaagcc cgcgtctatt
     4321 atttgccccg caagttcgct cctcttgagc attacgcgcg gggcgcgggc ttgcttggtg
     4381 cggcgcggtt tttcttcact gcggcggagt cggcgcggct cgtgtgcgcg ctcgcggcgt
     4441 agcgcccgtt aacctgaagt caatccgccg gtcggcgcac gacccgccct caagacacgg
     4501 tcgcgcatgg gggtataatc cacccgtcgg agagccgctc cggtattcat actactgcgc
     4561 cggctccaat caaccgatgc agacagcgcc cccgagggag gaatagtgtg aagacacgcc
     4621 ggctcttctt tggattcgcc ctctgctttc ttttttttcc accgggacac ctctggcgtg
     4681 gggcgcaagc ccaaacacca tcccggctga cgccgaaatt ccccctgcgc gggagcggcc
     4741 ttgagcttga gcggcgcacg caggccggca gcttcttcga tgtcgcggga cgcaaggcag
     4801 ccgtcttcgg ctacgagcac cgcccgctcg aagcgtgggt ctatcccttg aagctgctcg
     4861 acgacttcga gctttcgttt cgcgtcgaag gctacccgct ggcgtttcgc gcctcggaca
     4921 tggccgtgcg cgtcaacgtg cgcccggaag cgaccgtctt tacttattcg cacgccgcct
     4981 tcagcgtgcg gcagatcatc tacgcccccg tggacgagcc gggcgtcgtc atgctgctcg
     5041 atgtcgcgag cgtcctgccg atgaccgtcg aggtctcgtt ccggccacag ttgaaactgg
     5101 cgtggcccgc gggtctgatg acgggcaaca tcggctgggt tgaaaagcag cacgtgtact
     5161 acatcacgga agagtcccga cgctttgtcg gcgtggtcgg ctcgcccgcc gggcgcgacg
     5221 tgtccgtgat gccctatcag gaagagccgc gcgatgtccc ttcgcgcttc ctgatcgaga
     5281 cttcacccgc agagttgaag acgagcttta ttcccatcgt catcgcgggg agcgtcgagg
     5341 ggcgcgagaa agcgaaggcg gcttatgacc gtctgctcgg ctccgcccgg tctctctacg
     5401 aaaacaacgt cgcttactac gaacgccttc agcgcgagac gacgagcatc gagacgcccg
     5461 acgagagatt cgacacggct ttcgactggg ccaaagtcgg cgttgacaaa ggcatcgcga
     5521 ccaacccgtt tctcggcacg ggactgctgg cgggctttcg cacttcgggc cagagcgagc
     5581 ggcccggctt cgcgtggtac ttcgggcgcg acgcgctgtg gacggcgctg gcgatcaact
     5641 cctacggcga tttccgctcc gcgcgcacgg cgctcgaatt cctgaaaaag ttccagcgcg
     5701 cggacggcaa aatcccgcac gagatttcgc aaagcgcctc gctcatcccc tggtttaccg
     5761 attacgaata cccctgggcc agcgccgacg ccacgccgct ctacgtcatc gcccacgccg
     5821 attactggcg cgccaccgga gacaccgggt tcctccaaac gaactgggac tcgatccggc
     5881 aagcctatcg tttctcggag gccacggaca ccgacggcaa tcaactgatc gagaacacga
     5941 agttcggtca cgggtgggtc gaaggcggcc cgcttcgtcc tgtccacgaa gagatttaca
     6001 tgcaggggct gtggatcgaa gcttcgcgca gcatggccga gttggccgag gcgctcaagg
     6061 acggggccac cgcttcgcgc gcacgcgcgg ctgccgaacg cacgcgcgcc gcgatggagc
     6121 agacttactg gctcgcggat cgcggctttt atgctttcgg cacgcgcctg caaccgacga
     6181 acgaaactcc cgaagccgag ccggggccga accgtgccgc gcgtcaggcc cggatggaag
     6241 aactcgccac ggcgcgtttg attgacgaag acacggtgct gccggcggtg ccgctctggt
     6301 ggcacacgat gctggacgag cgcgcgcaat cccagataga tcatctgggc ggcggacgga
     6361 tggcgacgga ctggggggcg cgcatcatct ccgacgagag ccgtctctac gacccgctct
     6421 cttatcacta cggctccgtg tggccgctct tcaccggctg ggccagcatg ggcgcttacg
     6481 cttacggtcg cccccacgtc ggccatcaag cgctgatggc gaacgcgctg ctctcctaca
     6541 cgggggcgct gggctacgtg acggaactgc tgtccggcga tttcaacgcg ccgttcgggc
     6601 gctcgtcgca ccatcaggtc tggtcggagg cgatggtcgt cacgccggcg ttgcgcgggc
     6661 tgctcggcat tgaaatcgac gagggcggca aagagctgcg cttcgcgccg cagctacctg
     6721 cgaattggga tcgagtcgca gcactgaacg tcgccgcggg cgacgcgcgc tacgatctga
     6781 agctggcgcg ggcggcgggt cggctcacga tcacggtcac gcaaagaatt tccgccgcgt
     6841 ccggttcttc ctctttgtct cgacgggtcg agcggttcac gttcgcgccc gcctttccgc
     6901 tcgacgcgcg ggtgcgcaaa gtgacggtgc agggacgtgc gacaaacttc gaaatgaaaa
     6961 gcggcggcga cgtgcagcgc gccgaggtcg gtatcgaagc ccgtgagccg agcgtcgaga
     7021 tagtctttac ttatacggaa ggcacggacg tttatcacgt gccgcaaagc ctcacgcccg
     7081 gcgccgagag cgaggggctg cgcgtcttac gctcgcgcgc cgaccgggac gcgttgcgcc
     7141 tcctgctcga aggaatcggc ggtcgcactt acgccgtgcg cgtgcgaact ccgcaccaac
     7201 tcggcccggc ggacggcgtg tcggtgcagc cggatgacaa ctccgacccg cttctggccg
     7261 tcgcttttga aggcgcatcc ggttcctacg tccggcgcga agtgaccatc ccgctccggc
     7321 gcgcagccgc cgcgcggagt aagaggaagt gaattgctgt gacccggcgg gaggcgaact
     7381 gctgacgaag gagacaccac tatgacaaag acaccgcgcg tcacgaagtc ctttcacc
//
LOCUS       DBNGOH010001124         2788 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1637772, whole genome shotgun sequence.
ACCESSION   DBNGOH010001124 DBNGOH010000000
VERSION     DBNGOH010001124.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2788)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2788)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2788
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1637772"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..785)
                     /locus_tag="AC159P_21875"
     CDS             complement(<1..785)
                     /locus_tag="AC159P_21875"
                     /inference="COORDINATES: protein motif:HMM:NF013736.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HIR3698149.1"
                     /translation="MTTPAQTLSIERKIRMHEAREAIINRLAPNIHSEIDLDRFLRAI
                     VAELGRMMEVDRCDVLQLTPTGELRISHEWRAAEDVPSSLGTVLHVDIGQLVERFNLA
                     RPIRLDDISAPDLDPRVSLLPKSLGTRSLLIVPVVLDGNVLGLVGLHMTRAVRHWDDE
                     EVSFLQSIARQIAVGYQYARLYTDVQREAARTRALLEIANAINARSDFGEVTSHVLER
                     AIALVGADYCALGVVEPSGDTINLAAFKAAPRAETSGVRELIEA"
     gene            complement(1065..1559)
                     /locus_tag="AC159P_21880"
     CDS             complement(1065..1559)
                     /locus_tag="AC159P_21880"
                     /inference="COORDINATES: protein motif:HMM:NF013618.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008933 - peptidoglycan lytic
                     transglycosylase activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HIR3698150.1"
                     /translation="MTTGAVAAEDFIPADVPSSGDKELDRIIFRTGRDLGVDPRLLHA
                     VIWQESKYKNAAVSHAGAQGLMQLMPATAARFKCENRSDAKANIEAGTKYLRWLLKRF
                     NGDVTLALAGYNAGEGNVDKHAGVPPFDETQNYVRIITGRYGKNFHPTLAPVEAVSYF
                     RLAQ"
     gene            2203..2523
                     /locus_tag="AC159P_21885"
     CDS             2203..2523
                     /locus_tag="AC159P_21885"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HIR3698151.1"
                     /translation="MPIYEYSCQKCGEHMEVMQKISDKPLVRHPKCGGRLVKEWSRTG
                     FQFKGSGWYVTDYAGKKSDAKEDGKEAKETKTEVAESKPQGDKPADAKPAKSKKKAPS
                     TGND"
     gene            2538..>2788
                     /locus_tag="AC159P_21890"
     CDS             2538..>2788
                     /locus_tag="AC159P_21890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3698152.1"
                     /translation="MRAHLKRISCGLVFGALLAGLAAGQAGRRPAPTAGDGPMKMDRA
                     IQLNVVAARPSGSETPVTAKDIALYDGGMEQQVQTFAYDP"
BASE COUNT          545 a          823 c          957 g          463 t
ORIGIN      
        1 gcttcgatca gctcgcgcac gcccgaagtc tctgcgcgcg gcgcggcctt gaaggcggcg
       61 aggttgatcg tgtcgcccga aggctcgacc acgccgagcg cgcagtagtc ggcgccgacg
      121 agcgcgatgg cgcgctccag cacgtgcgac gtgacctcgc cgaagtcgga gcgcgcgttg
      181 atggcgttgg cgatttccag gagggcgcgc gtgcgcgcgg cctcgcgctg cacgtccgtg
      241 tagaggcgcg cgtactggta gccgacggcg atctgccgcg cgatggactg gaggaacgag
      301 acctcctcgt cgtcccagtg gcgcacggcg cgcgtcatgt ggaggccgac gaggccgagc
      361 acgttgccgt cgagcactac ggggacgatg agcagcgagc gcgtgccgag gctcttcggc
      421 aggaggctga cgcgcgggtc gaggtcgggc gccgagatgt cgtcgaggcg gatggggcgc
      481 gcgaggttga agcgctcgac gagctggccg atgtccacgt gcaagacggt gccgaggctc
      541 gaaggcacgt cctcggcggc gcgccactcg tggctgatgc gcagctcgcc ggtcggcgtc
      601 agttgcagca cgtcgcagcg gtcaacctcc atcattcgcc ccagctcggc gacgatggcg
      661 cgcaggaagc ggtcgaggtc gatctccgaa tggatgttcg gcgcgaggcg gttgatgatc
      721 gcctcgcgcg cctcgtgcat cctgatcttg cgctcgatgg agagcgtctg cgccggcgtc
      781 gtcactgtgg ttctgcgctc ggaagacaag acttgaaaac acccttcgac gaaaagtcgg
      841 gtgcgcgccg gagtcgtgtt aaagctctgg aggagagaac gcgccgcgcg gttaacgcct
      901 cgggccgcaa aggattttcg ccgcgcgcga atatagaaag gtgaaagtca aaagtcaaaa
      961 ggcaaaagcg aagtcgagag gcagcgcgcc cctcgtcgag agggggatga ggagcagcgt
     1021 gctcctcatc ccccttcact tttgcctttc gactttcgcc ttaactactg cgcgagccgg
     1081 aagtagctga ccgcctcaac gggcgcgagc gtcgggtgga agttcttgcc gtagcggccc
     1141 gtgatgatgc ggacgtagtt ctgcgtctcg tcgaagggcg gcacgcccgc gtgcttatcg
     1201 acgttgcctt cgcccgcgtt gtagcccgcg agcgcgagcg tcacgtcgcc gttgaagcgc
     1261 ttcagcaacc agcgcaggta cttcgtcccg gcctcgatgt tggccttcgc gtccgaccgg
     1321 ttctcgcact tgaagcgcgc ggcggtcgcg ggcatgagct gcatcagccc ctgcgcgccg
     1381 gcgtgcgaga cggcggcgtt cttgtacttc gactcctgcc agatgaccgc gtgcagcaga
     1441 cgcggatcga cgccgagatc gcggcccgtg cggaagatga tgcggtcgag ttccttgtcg
     1501 cctgacgagg gcacgtccgc cgggatgaag tcttcggcgg caacggcgcc ggtggtcatc
     1561 gccgccgcag agggggcgaa ggcacgcgta taaggactgt caacgacggt cgagaaaatg
     1621 aacaacgcga cgagcgcgag ggggaaagac ttcagggcgc gaaaacgggg aaggggtcgg
     1681 gtcaggtgca ttattggtcg ttctccatgt gagctgtcgg ccttcgaaaa tcgcctcggg
     1741 ccggcgacga ttgcagcaga cacactgcaa gggtttgggg gctgcgcatg gtggaaacga
     1801 tctgttctgc atctcaaaga gcagcactgt cgaaaccgcg ggtatgatgg gtaagaccct
     1861 tatccagttg ctttcgcgga cggaatttat tcgccgcaag ttctttccgt acacgtactt
     1921 aacccgaacg ctccaagtcg ttgaaaggtc gaattttgac ccctgttcgg tcagcatgaa
     1981 cccaaaatcg cggggcgcgc gagaccctgc gtgtaccgaa cgcgcgcaaa gaaactcgag
     2041 gtcggggaga aaaagtcgtg cggttcaatc tgacgtagca gacgcgaacg cgccttcgcc
     2101 gcatcgaacg tccgaccgag ttgcacgccg actttttgtc cgtataattt cgggtgaagt
     2161 attaaacgga aaccaagctt gaagaggccg gttaaaagaa gcatgcccat ttacgaatac
     2221 agttgtcaga agtgcggcga gcacatggag gtgatgcaga agatctccga caagccgctc
     2281 gtgcgccacc ccaagtgcgg cgggcggctc gtcaaggagt ggtcgcgcac gggcttccag
     2341 ttcaaaggct ccgggtggta cgtgaccgac tacgcgggga aaaagtcgga cgcgaaagag
     2401 gacgggaagg aagcgaagga aacaaaaacc gaagtcgcgg aatcgaagcc gcagggcgac
     2461 aagcccgcgg acgcgaagcc cgcgaagtcc aaaaagaagg ctccctccac cggcaacgat
     2521 tagattaagg actagccgtg cgcgcacacc tcaaacgaat ctcctgcggt ctcgtcttcg
     2581 gcgccctgct ggccggcctc gcggcggggc aggcgggccg acgccctgcg ccgacggcgg
     2641 gcgatggccc gatgaagatg gatcgggcca tccagttgaa cgtcgtcgcc gcgcgcccga
     2701 gcggctccga gacgcccgtc acggcgaagg acatcgcgct ctacgacggc ggcatggagc
     2761 agcaggtgca gaccttcgcc tacgaccc
//
LOCUS       DBNGOH010001125         2451 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_5121150, whole genome shotgun sequence.
ACCESSION   DBNGOH010001125 DBNGOH010000000
VERSION     DBNGOH010001125.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2451)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2451)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2451
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_5121150"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            242..724
                     /locus_tag="AC159P_21895"
     CDS             242..724
                     /locus_tag="AC159P_21895"
                     /inference="COORDINATES: protein motif:HMM:NF025887.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR3698153.1"
                     /translation="MRRILALVVLIVAAAPLALGQAQGKPAAEKMAAGGNAEQELMQL
                     EQKLADALVKGDFATFGSHLADDVVLTDPGGVVNTRAQFEADVKSGALKIESSKIEDL
                     KVKTHGDVAIVTYKTTDKGTYKGSDISGDYRWIDVFVKKDGKWWLVAGQGTRIQKMPQ
                     "
     gene            complement(847..1866)
                     /locus_tag="AC159P_21900"
     CDS             complement(847..1866)
                     /locus_tag="AC159P_21900"
                     /inference="COORDINATES: protein motif:HMM:NF014600.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HIR3698154.1"
                     /translation="MKRFELHFAVFFVARATVAAFFALDAGAGRVRSQEAARRDAAAA
                     PAEPGLKTNAVESGLKPTAAVIERRAEPVAASTSAASAFNAAATRNALLRVELDWTFG
                     GKQQRGWHLYAPLINRLIEAEHDPATGDFASALSRWQTRAGLRPSGVLDQESLYAMVS
                     AWQGARLKQRGYPQPDQLLTAPSADFYDPARPDELRQVERETYAAYKRMVAAAAQDPS
                     LRLAANAAGELAPAEKYLKIISSFRSREYQARLRRMEPHAGRAALAVHHSPHFTGRAL
                     DLYVGGEPVETKDSNRAIQVQTAVYRWLVRNADRFGFRPDYYEPWHWEYVGTATADAK
                     PHTAR"
     gene            2159..2368
                     /locus_tag="AC159P_21905"
     CDS             2159..2368
                     /locus_tag="AC159P_21905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3698155.1"
                     /translation="MLESNSAASVSPNVSFELENGSTFTFNFQMDGRVTVIGFQEGRA
                     VTGALTAEQVERLRDAFSFYLDKRK"
BASE COUNT          449 a          761 c          850 g          391 t
ORIGIN      
        1 agaaaagaag aagggcggat gaagaagagg gagagtcgaa cgcttcgact ctccctcgac
       61 tcgtctctgc tctgcttttg tccctctcta tctctccgcg tcacgtcggg cgagcgcacc
      121 gacccgcgcg cgcggccttc ggttctccat tgacttgcca ccctgtttgg gatagaacgc
      181 ccacgcttta accggccccg acaacgcttg gcagacccac acccaaaaag gagccactac
      241 catgagacga atacttgccc tcgtcgtgct gatcgtcgcc gccgcgcctt tggctttagg
      301 gcaggcgcaa gggaaacccg ccgccgaaaa gatggccgcg ggcggcaacg ccgaacagga
      361 gttgatgcaa ctggaacaga agttggccga cgccctcgtg aagggcgact tcgccacgtt
      421 cggcagccac ctcgcggacg acgtcgtgct cacagacccc ggcggcgtgg tcaacacccg
      481 ggcgcagttc gaggccgacg tgaagtcggg cgccctcaag atcgagtcct cgaaaatcga
      541 agacctgaag gtgaagaccc acggggacgt ggccatcgtc acttacaaga cgacggacaa
      601 gggcacctac aaggggagtg acatcagcgg cgactaccgc tggatcgacg tgttcgtgaa
      661 gaaggacggc aagtggtggc tcgtcgccgg gcaggggacg cgcatccaga agatgccgca
      721 gtaagcgccc gtgacggccc cgcgccacac gcgaaaagcc ccgccccctc gggcggggct
      781 tttcatcgat gccccgaaaa ctctcccgac attttcccgc cggacgaaaa acccgactcg
      841 cggagatcag cgtgcggtgt gaggcttggc gtcggcggtc gcggtgccga cgtattccca
      901 gtgccaaggc tcgtagtagt cggggcggaa gccgaagcgg tcggcgttgc ggacgagcca
      961 ccggtagacg gcggtctgca cctgtatggc gcggttggag tctttggtct cgaccggttc
     1021 gccgccgacg tagaggtcga gggcgcggcc cgtgaagtgc gggctgtggt ggacggcgag
     1081 ggcggcgcgg cccgcgtgcg gctccatccg gcgcaagcgt gcctgatact cgcgcgagcg
     1141 gaacgaggag atgatcttca aatacttttc ggcgggcgcg agttcgcccg cggcgttcgc
     1201 cgcgagcctg agcgaggggt cttgtgccgc cgccgcgacc atgcgcttgt aggcggcgta
     1261 ggtctcgcgc tcgacctggc gcagctcgtc ggggcgcgcg gggtcgtaga aatcagcaga
     1321 cggcgccgtg aggagctggt cgggctgcgg gtagccgcgc tgcttgagcc gcgcgccctg
     1381 ccaggcggag accatcgcgt agagcgactc ctgatcgagc acgccgctgg gccgcaggcc
     1441 cgcgcgcgtc tgccagcgcg agagcgcgga ggcgaagtcg ccggtcgcgg ggtcgtgctc
     1501 ggcctcgatg aggcggttga tgagcggggc gtagagatgc cagccgcgct gctgcttgcc
     1561 gccgaacgtc cagtcgagtt cgacgcgcaa aagcgcgttg cgcgtggccg cggcgttgaa
     1621 ggcggaagca gcggaagtgc tcgcggcgac cggttcggcg cgacgttcga tgacggcggc
     1681 ggtcggcttc aagcccgact cgacggcgtt cgttttgagg cccggctccg cgggcgcggc
     1741 ggcggcgtcc cgtcgcgcgg cctcctgcga ccggacgcgg cccgccccgg cgtcgagcgc
     1801 gaagaaggcc gcgacggtcg cccgtgcgac gaagaagacc gcgaagtgga gttcgaaacg
     1861 cttcatgcct gtcgtgttcg aatgagcgaa actttacggg tgagggcggc ttgtacggct
     1921 gacagagtat cagatgcttt ggcgggggtc acggttcgta aggcgccggg atttttagtg
     1981 tcccgagcgg ctacggtcaa aatgcgcggc ggcgcggcgg ccaaaatgcg cggcgcggcg
     2041 gagtctggta aactaacgct ttcgcaagcg taggacagcg cctccccgaa gaacggcccg
     2101 tcagccgctc ggccaacaac cagcgacatc tacttcagca cggacggaga ttgacaagat
     2161 gcttgagtcg aacagcgccg cgagcgtttc cccgaacgtc tcgttcgagc ttgagaacgg
     2221 cagcaccttc accttcaact tccagatgga cgggcgcgtc accgtcatcg gctttcagga
     2281 gggtcgcgcc gtcaccggcg cgctcaccgc cgaacaggtc gagcgcctgc gcgacgcctt
     2341 cagtttctac ctggacaaga ggaaatgacg gaagggggaa aaggggaaga ggggaaaagg
     2401 aaaagcgaaa cctcttccgc cttccccttt tcgccttttc ccctaaagct t
//
LOCUS       DBNGOH010001126         1981 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4202887, whole genome shotgun sequence.
ACCESSION   DBNGOH010001126 DBNGOH010000000
VERSION     DBNGOH010001126.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1981)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1981)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1981
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4202887"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..868
                     /locus_tag="AC159P_21910"
     CDS             <1..868
                     /locus_tag="AC159P_21910"
                     /inference="COORDINATES: protein motif:HMM:NF019040.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HIR3698156.1"
                     /translation="YMESGTPGRKSTPDGWLNRYLQAKKDEKANPFRAVAMTQNLPRA
                     LYGRAPALAIQNLNDFSLRAGAQTANVQGGFENIYEQSVNDVLRGTGKETFEAVKFLK
                     QVNPAQYKPENGAQYPRGRYGEALLQIAQLIKAGVGLEVAFTDSGGWDTHRGQGGARG
                     QLAALLQQFGAGIKALTTDLGPERMQDVVILTMTEFGRTVRQNGTAGTDHGHASALFV
                     VGGSVRGGRVYGDWPGLKPEQLHEERDLALTTDFRDVFGEVASKHLGGVDLKKVFPGH
                     AGGASKFRGLLG"
     gene            1215..>1981
                     /locus_tag="AC159P_21915"
     CDS             1215..>1981
                     /locus_tag="AC159P_21915"
                     /inference="COORDINATES: protein motif:HMM:NF024968.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR3698157.1"
                     /translation="MSGRYDFSADLILVCNSLDAGGIERVVSTLANEWSRRGRKVCVI
                     TMHDRRRFYALAPAIHHVVIDRAGLARLADLLRWLAARLRGSAPPRFWLLRVLGGVLH
                     RLFSRRLYRAYAALVYTYEAWALRRVLKRVETPVVVSLGTPTNIVTVRACRGLGRRLV
                     ISERNDPKRLPRIRNWDALARRFYNRADLVTANTRGALRDMSEYVETGKLAFVPNPLV
                     LANGHDNGGGGNGNGHAPAPAPTILIVARLVWDKAHEV"
BASE COUNT          327 a          686 c          673 g          295 t
ORIGIN      
        1 ctacatggag tcgggcacgc ccggcaggaa gtccacgccc gacggctggc tcaaccgcta
       61 cctgcaagcg aagaaggacg agaaggccaa cccgttccgt gccgtcgcca tgacgcagaa
      121 cctgccgcgc gcgctctacg gtcgcgcgcc cgctctcgcc atccagaacc tgaacgactt
      181 ctcgctccgc gcgggcgcgc agaccgcgaa cgtgcagggc ggcttcgaga atatttatga
      241 gcagagcgtc aacgacgtgc tgcgcggcac gggcaaagag acgttcgagg ccgtcaagtt
      301 tttgaagcag gtcaaccccg cgcagtacaa gccggagaac ggcgcgcagt acccgcgcgg
      361 tcgctacggc gaggcgctct tgcagatcgc gcagctcatc aaggcgggcg tcggtctgga
      421 ggtggccttc acggactcgg gcggctggga cacgcaccgc gggcagggcg gcgcgcgcgg
      481 ccagctcgcc gcactcttgc agcagttcgg cgcggggatt aaagcgctca cgacagacct
      541 cggccccgaa aggatgcagg atgtcgtcat cctgaccatg accgagttcg ggcgcaccgt
      601 ccggcagaac ggcaccgccg gcacggatca cggccacgcc agcgcgctct tcgtcgtcgg
      661 cggctcggtg cgcggcgggc gtgtctacgg cgactggccc ggcctcaagc cggaacagct
      721 ccacgaggag cgcgacctcg cattgacgac cgacttccgc gacgtgttcg gcgaagtcgc
      781 ctcgaagcac ctcggcggcg ttgacctcaa gaaagtcttc cccggccacg ccggcggcgc
      841 ctcgaagttc cgcggcctgt tgggctgagc gcgagtccgg gcggacgaga gaagggggcg
      901 ctgcgtcgca ggtggcgcgt ggtcggcgtc gggcaaaaac cccgtgccgg gtcggcggcg
      961 cgcaggtggc gggttcggta cggagctgtt cgccaccccg aagacggcgg atgaggcgtc
     1021 acgcgtgtcg aagccaggct cggcgtgtag gaaagcgcga cccccggacg acgtgcttcg
     1081 gagcgccggg attatacacg attgcggaga cgcggcggcg ccaaaagcgg cggcggggcc
     1141 gcgcctcgcg cggtgtggta tcatccacgg cacctcgccg ttctccgact ccattgtgac
     1201 aatgtgaagc cggtatgtcc ggtcgctacg acttctccgc cgacctcatc cttgtttgca
     1261 acagcctcga cgccgggggc atcgagcgtg tcgtctccac cctcgccaac gagtggagcc
     1321 ggcgcgggcg caaggtctgc gtcatcacga tgcacgaccg ccgccggttt tacgccctcg
     1381 caccggccat ccatcatgtc gtcatcgacc gggccgggct ggcccgtctc gccgacctgc
     1441 tgaggtggct cgcggcgcgt ctgcgcggta gcgccccgcc acgcttctgg ctgctccgcg
     1501 tcctcggcgg cgtgcttcac agactcttca gccggaggct ctacagggct tacgccgccc
     1561 tcgtctacac ctacgaggca tgggcactgc ggcgcgtgtt gaagcgcgtc gagacgcccg
     1621 tcgtcgtctc tctggggacg cccaccaaca tcgtcacggt gagggcgtgt aggggcttgg
     1681 gccggcgcct cgtcatctcc gagcgcaacg accccaaacg gttgccgcgg atcaggaact
     1741 gggacgcgct ggcacgcaga ttctacaatc gtgccgacct cgtgacggcg aacacgcgcg
     1801 gggcgctccg cgacatgagc gaatacgtcg aaacggggaa actggccttc gtccccaacc
     1861 cgctcgtgct cgcgaacggc cacgacaacg gcggcggcgg caatggtaac ggccacgccc
     1921 ccgcccccgc gccgaccatc cttatcgtcg cccgcctcgt gtgggacaag gcccacgagg
     1981 t
//
LOCUS       DBNGOH010001127         5643 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4571043, whole genome shotgun sequence.
ACCESSION   DBNGOH010001127 DBNGOH010000000
VERSION     DBNGOH010001127.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5643)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5643)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5643
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4571043"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..495)
                     /locus_tag="AC159P_21920"
     CDS             complement(<1..495)
                     /locus_tag="AC159P_21920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3698158.1"
                     /translation="MSTRKDVSTLLLALAVFCLCAPAGSHAASSAALRRGVRRTKSAE
                     FVRQEEVVSFPRAGRVRVRAIEVPRTRPRLEFVSEKTGRRLLSFSVGSSDARAYRIES
                     YETPMNPLVRFKVLEAAGLPGPLVFAVAVKPGGTDHGFETNIIAEAGGRLKLLTPAPL
                     TNNIQ"
     gene            complement(715..1926)
                     /locus_tag="AC159P_21925"
     CDS             complement(715..1926)
                     /locus_tag="AC159P_21925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3698159.1"
                     /translation="MNRRSIRFIAAAFVCAALSLCALVGEGRGQDAEKGGGRGETVAF
                     DSDRWVVSGTGVKREEHLGRRSLFLAGGAFAHLKDVEFEDGTIEVDVAASQPRAFLGV
                     AFRFLSREEHEMFYLRPQKSGLADATQYTPAFNAAHPWQLYSGKGFTAAAEIPRDRWL
                     HVRIEINGLAGRVYLDNAAEPVLVIEDLKRGHGKGSVGLWGSAMGGHFADFKFTAAKP
                     SATRTAPQTQPTAAGVLTKWELSDAFDTAEKSLETLPAADAWKAMRWQAVTTEAPGIL
                     VIDRYRKGHNVVPAFAGDPNLRLQKARGPKAVYARARLHSEREQVKKLHFGYSDEVTV
                     FLNGQPLFTGKSAFRFRDPGFLGIVDVENDAVHLPLRKGANELVLAVSEYFGGWGFTC
                     RLQDTAGITLD"
     gene            complement(2052..2900)
                     /locus_tag="AC159P_21930"
     CDS             complement(2052..2900)
                     /locus_tag="AC159P_21930"
                     /inference="COORDINATES: protein motif:HMM:NF014376.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HIR3698160.1"
                     /translation="MKAGDEREVINVWRPADVGGLELHRGIGVARPVARHWHEEFQLC
                     LIEAGAGELIYRGEHHPTPAASLFVVHPGEIHSNRAYDRRGCSYRTLFVEPGLLRRTA
                     AEVEGRDRDVPFFPESVVSDREVLALFVRLHVALEVPASGLERESLLLAFSAKLIARF
                     GERRTGDGAFKAKRASVARAREYLTEHYAENVCLEQLARLVGLSPFHFSRLFSDEVGM
                     PPHAFQTQVRVARAKALLLGGWPISQAAAHTGFADQSHLTRHFKRLVGVTPGQYRQGS
                     KNVQDA"
     gene            3017..4945
                     /locus_tag="AC159P_21935"
     CDS             3017..4945
                     /locus_tag="AC159P_21935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3698161.1"
                     /translation="MCVILRRKIFANSILKVHRRVHTMRTLPFSNSKINAAMFLAATA
                     LCVLAAFGLAGAKRPRAGAAEARPASSRQTATVVLDDSAFYEHMPKGTIITQVRAQGP
                     GQQQGAGQQPPPQVALPQGAWLFKNSGLVMRSQAYLYAKADIPEAGAYHLFVRSHGSR
                     DSSFRVSVGDRQPAERFGDEPLRWKRGGTFELKKGLTDVVLSRVVLGEAVGSTFDVLV
                     LTKDAGFGEDDLKPLELHEDVALLKEYTIPRSSAVKFGDVDGDGRTDFFVVTGNYDGR
                     VFDHDGRELWSYQNEQEGARARAGFEAPGLVWDFDRDGAAEVVHYRLAGGKEWLVVSD
                     GRTGAVRHKTEWPTRPMPHEYNNFRLAVAQLSGDYPSNILAFTDSGGTISITAYTADL
                     KQLWQHVERRKKDHLGHYAYAVDLNKDGVDEVVASPVVLDARGRVVWSRFDLFDDNHD
                     HCDSFRFHDLDGDGELELLAPLSEAGVVVFRARDGALLWRHPAEHSQQLEVGDFLRGV
                     AGPHVAVNARTYARNGEAGLGGQVHWFDARGKLLSKWPANPLNGNPDFVKGDWRGDGR
                     AELFWYRFKLTEEGRGVLYFKQDAYHMFDFMGTGADQVIARGANTLQVYGYRHAKPKT
                     GVRRDVSYRKRVANHTHY"
     gene            complement(4970..>5643)
                     /locus_tag="AC159P_21940"
     CDS             complement(4970..>5643)
                     /locus_tag="AC159P_21940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3698162.1"
                     /translation="QGRSTGYEEYTGALAAPRRHVYVLDGEGRRVEYRIVQPDGAAGE
                     KYLYKYDAKGRLSEEALHEHKGALISRNAFAYDEEGRQVSQTRYNSDGSVSSTSSTSY
                     DAHGRPVERVRHEGDILTYRVRYTYDAKGRVVAQETVGSVVEADVSPSEAHAPGRVIY
                     VYKGKEWPKGALAYAPDGSLRERVEIEYDSRGNWTRKTRLMRPGAASQSRRVEYRAIT
                     YFRGE"
BASE COUNT          908 a         1867 c         1934 g          934 t
ORIGIN      
        1 ctgtatgttg ttcgtgagcg gcgcgggggt gagcagcttc aggcgaccgc cggcctcggc
       61 gatgatgttc gtctcgaagc cgtgatcggt gccgccgggc ttgaccgcca cggcgaagac
      121 gagcggaccg ggcaggcccg ccgcctccag caccttgaag cgcaccagag ggttcatcgg
      181 cgtctcatac gactcgatgc ggtaggcccg cgcgtccgag gagccgacgc tgaacgagag
      241 gagcctgcgc cccgtcttct ccgaaacgaa ttccagccgc gggcgcgtgc gcggcacctc
      301 gatggcgcgc acgcgcacac gccccgcgcg cgggaagctc acgacctctt cctggcggac
      361 gaactccgcc gacttcgtcc gccgcacgcc gcgccgcagc gccgcggacg aagccgcgtg
      421 agacccggca ggcgcgcaga ggcagaagac cgccagcgct aacagcagcg tcgagacatc
      481 ttttcttgtg ctcatcgatt ggaggccgtc gttaccgtac gagatagacg atacgcccga
      541 cgggtcttca ttatacacgc gacgcgcgga agataagggc gaagcgggga agaggggaag
      601 aggggaaaag gggaaggggg aagacgagaa gcagcgcgcc tctcatcttc gatcaaggat
      661 gagaggcacg gtgcttccca tcctcccttt tcgcctcttc cccttttccc cacttcagtc
      721 gagtgtgatg cccgcggtgt cctgcaagcg gcaggtgaag ccccagccgc cgaagtattc
      781 ggagacggcg aggacgagtt cgttggcgcc ttttctgagc ggcaggtgga cggcgtcgtt
      841 ctcgacatcg acgatgccga ggaagccggg gtcgcggaag cggaaggcgc tcttgccggt
      901 gaagagaggt tggccgttga ggaagaccgt cacctcgtcg ctgtagccga agtggagttt
      961 cttgacctgc tcgcgctccg agtggagacg ggcacgcgcg tagacggcct tcggcccgcg
     1021 cgccttttgg agccgcaggt tcgggtctcc ggcgaaagcc gggacgacgt tgtgcccttt
     1081 gcggtagcgg tcgatgacga ggatgccggg cgcctcggtc gtcaccgcct gccagcgcat
     1141 cgccttccat gcgtccgcgg cgggcagcgt ctccaaactc ttctccgccg tgtcgaaggc
     1201 gtcggacagc tcccacttcg tcaacacgcc cgccgcggtc ggttgagttt gcggggccgt
     1261 gcgcgtcgca gacggtttgg cggcggtgaa tttgaagtcg gcgaagtggc cgcccatcgc
     1321 gctgccccag aggccgacgg agcctttgcc gtggccgcgt ttgaggtctt cgatgacgag
     1381 caccggctcc gccgcgttgt cgaggtagac gcggcccgcg aggccgttga tctcgatgcg
     1441 gacgtgcagc cagcggtcgc gcggaatctc cgcggcggcg gtgaagccct tgccgctgta
     1501 gagctgccac gggtgcgcgg cgttgaaggc gggcgtgtac tgcgtcgcgt ccgcgaggcc
     1561 cgacttctgc gggcggaggt agaacatctc gtgctcttcg cgcgacagga agcggaaggc
     1621 gacgccgaga aaggcgcgcg gctgcgaggc ggcgacatcc acctcgatcg tcccgtcctc
     1681 gaactccacg tccttcaggt gcgcgaacgc gccgcccgcg aggaagagac tccggcgacc
     1741 gaggtgctct tccctcttca cgccggtgcc gcttactacc cagcggtcgg aatcgaaggc
     1801 gaccgtctcg ccgcgcccgc cgcccttctc tgcatcctgc ccccgccctt cgccgacgag
     1861 cgcgcagagc gagagcgcgg cgcacacgaa cgccgccgcg atgaaacgaa tgcttcttct
     1921 gttcatggca actccctctc aaggtgaatg gtggatggta aatagtgaat ggtgttgagc
     1981 aggcttatcc atttaccatt tactattcac tattcacctg ccttcttgcg ggagtttaaa
     2041 ggcgagcgcc ttcaggcgtc ttgaacgttc ttgctgccct gccggtactg gcccggcgtg
     2101 acgccgacca gccgcttgaa gtggcgcgtg aggtggctct ggtcggcgaa gcctgtgtgc
     2161 gccgccgcct gcgagatcgg ccagccgccg agcagcagcg ctttggcgcg cgccacgcgc
     2221 acctgcgtct ggaacgcgtg cggcggcatc ccgacctcgt ccgagaagag gcggctgaag
     2281 tggaaggggc tgaggccgac gagccgcgcc agttgctcaa ggcaaacgtt ctccgcgtag
     2341 tgctcggtca ggtattcgcg cgcgcgcgcg acgctcgccc gcttcgcctt gaaggcgccg
     2401 tcgcccgtcc gccgctcgcc gaaccgcgcg atgagtttgg ccgaaaaggc gagcagcagc
     2461 gactcgcgtt cgaggcccga cgctggaacc tccaacgcca cgtggagccg cacgaacagc
     2521 gccagcacct cgcggtcgga caccaccgac tcggggaaga acggcacgtc gcggtcgcgc
     2581 ccctcgacct ccgccgccgt gcgccgcagc agccccggct cgacgaacag cgtgcggtag
     2641 ctacagcccc ggcggtcgta agcgcggttc gagtgaatct cgcccggatg cacgacgaac
     2701 agactcgccg cgggcgtcgg gtggtgctcg ccgcgataga tcagctcgcc cgcgcccgcc
     2761 tcgatcagac acaactggaa ctcctcgtgc cagtggcgcg ccacgggccg cgccacgccg
     2821 atgccgcggt gcagctccaa cccgcctacg tcggctggcc gccacacgtt gatgacttcg
     2881 cgctcgtcgc ccgctttcat gccccgacag ttctatcatc aaatcgaacc gcccgcacga
     2941 agaatcgccg gcgcgtaccg atgggtggaa ttagttccgt gtgacctcgg cgccgcgttc
     3001 ctacaaacgg cgcgccgtgt gtgtgatact gcggcggaag attttcgcca actccattct
     3061 caaggttcat cgacgggtgc acacgatgcg gacgcttccc ttctccaact ctaaaatcaa
     3121 cgcggccatg tttctcgccg cgaccgcgct gtgcgtgctt gcggcgttcg gcttggcggg
     3181 agcgaagcgg ccccgcgcgg gtgcggcaga ggcgcggccc gcttcgtcgc ggcagacggc
     3241 gacggtcgtg ttggacgact cggcgtttta cgagcacatg ccgaaaggca ccatcatcac
     3301 gcaggtgcgc gcgcaagggc cggggcagca gcaaggcgcc ggccagcagc cgccgccgca
     3361 ggtcgcgctg ccgcagggcg cttggctgtt caaaaactcg ggcctcgtga tgcgctcgca
     3421 ggcttacctc tacgcgaagg cagacatccc cgaggcaggg gcgtatcacc tgttcgtgcg
     3481 gagccacggc agcagggaca gctctttccg cgtgtcggtc ggggacagac agcccgccga
     3541 gcgtttcggc gacgagccgc tcaggtggaa gcggggcggc acgttcgagt tgaagaaggg
     3601 cttgacggat gtcgtgctga gccgcgtcgt gctcggcgag gcggtcggct cgacgttcga
     3661 cgtgctggtg ctgacgaagg acgccggatt cggggaagac gatctgaagc cgctggagct
     3721 gcacgaggac gtggcgctgt tgaaggagta cacgatcccc cgttcgtcgg cggtgaagtt
     3781 cggcgacgtg gacggcgatg gccggacgga cttcttcgtc gtgacgggca actacgacgg
     3841 gcgcgtcttc gaccacgacg ggcgcgagct gtggtcttac cagaacgagc aggagggcgc
     3901 gcgggcgcgc gcggggttcg aggcgccggg gctggtctgg gacttcgacc gcgacggcgc
     3961 ggcggaggtc gtccactacc gcctcgcggg cggcaaggag tggctcgtcg tctcggacgg
     4021 gaggacgggc gccgtccggc acaagaccga gtggccgacg cgcccgatgc cgcacgagta
     4081 caacaacttc aggctcgccg tcgcgcagct ctcgggcgac tacccgagca acattctggc
     4141 cttcacggac tcgggcggca ccatctcgat cacggcttac acggcagacc tcaagcagct
     4201 ctggcagcac gtcgagcgga ggaagaaaga ccacctcggc cactacgcct acgcggtgga
     4261 tttgaacaag gacggcgttg acgaagtcgt cgcctcgccc gtcgtgctcg acgcgcgggg
     4321 gcgcgtcgtc tggagccgct tcgatctgtt cgacgacaac cacgaccact gcgacagctt
     4381 ccgctttcac gacctcgacg gcgacggcga gctggagctg ctcgcgccgc tcagcgaggc
     4441 gggcgtcgtc gtcttccgcg cgcgcgacgg cgcgctcctg tggcgccacc cggcggagca
     4501 ctcgcagcag ttggaggtcg gcgacttcct gcggggcgtt gccgggccgc acgtcgccgt
     4561 caacgcgcgc acctacgcgc gcaacggcga ggccggactc ggcgggcagg tgcactggtt
     4621 cgacgcgcgc gggaagctgc tctcgaagtg gcccgccaac cctctgaacg gcaaccccga
     4681 cttcgtcaaa ggcgactggc ggggcgacgg gcgcgcggag ctgttctggt acaggttcaa
     4741 gctgacggaa gaggggcgcg gcgtgctcta cttcaagcag gacgcctacc acatgttcga
     4801 cttcatgggc acgggcgccg accaggtcat cgcgcgcggc gccaacaccc tgcaagtcta
     4861 cggctaccgg cacgccaagc cgaagaccgg cgtcaggcgc gacgtgagct accggaaacg
     4921 ggtcgccaac catacacact actagcgcgg aatgccggcg agggtggcgc tactcgccac
     4981 ggaagtacgt gatggcgcgg tattcgacgc ggcgcgattg gctcgcggcg ccgggccgca
     5041 tcagccgcgt cttcctcgtc caattcccgc gcgagtcgta ttcgatctcg accctctccc
     5101 tgagcgagcc gtccggggcg taggccagcg ctcccttcgg ccactccttg cctttgtaga
     5161 cgtagattac cctgccgggc gcgtgcgctt ccgaggggga cacgtcggcc tcgaccaccg
     5221 acccgacggt ctcctgcgcg acgaccctgc ctttggcgtc gtaggtgtag cggactctgt
     5281 aggtgagaat gtcgccctcg tgccggacgc gttcgaccgg cctgccgtgg gcgtcgtacg
     5341 aggtggaact cgtggaagag acggagccgt ccgagttgta tctggtctgc gagacctgcc
     5401 gcccttcttc gtcgtaggcg aaggcgttgc ggctgatgag cgcgcccttg tgttcgtgga
     5461 gcgcttcctc tgagaggcgc cccttcgcgt cgtacttgta aagatacttt tcgcccgcgg
     5521 cgccgtccgg ctggacgatc ctgtactcga ccctgcgccc ttccccgtcc agcacgtaga
     5581 cgtgtctgcg gggggcggcg agcgcgccgg tgtactcctc gtagccggtg ctcctgccct
     5641 gcg
//
LOCUS       DBNGOH010001128         5410 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1272699, whole genome shotgun sequence.
ACCESSION   DBNGOH010001128 DBNGOH010000000
VERSION     DBNGOH010001128.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5410)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5410)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5410
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1272699"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..151)
                     /locus_tag="AC159P_21945"
     CDS             complement(<1..151)
                     /locus_tag="AC159P_21945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011531158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HIR3698163.1"
                     /translation="MQTKKGQKGFSLIELLIVVAIIGIVAAIAIPNLLASRRAANEAS
                     AISSMR"
     gene            complement(596..1725)
                     /locus_tag="AC159P_21950"
     CDS             complement(join(596..1293,1293..1725))
                     /locus_tag="AC159P_21950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611351.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HIR3698164.1"
                     /translation="MPKKYQITLTPEERHSLEQMVRSGQTPARTLTRARVLLKADCGE
                     GRIGWTDEQIVEALDVGRATVERIRQRFVEESFETALYSRRPGRRYPHKLDGEAEAHL
                     VALACTQPPAGRARWTVRLLTGRLVELGVVEAVSRETVRRVLKKNELKPWLVKQWCIP
                     PQQNAAFVAAMEDVLEVYTRPFDPLRPQVCMDEKAVQLIGETRLPLPVAPGREVRCDF
                     EYVRRGVTNLFMLFEPLAARREVEVRAKRGARQWAEVMRELVDVHYPEAERIVLVLDN
                     LNTHAPSSLYEVFRPEEARRIARKLELHYTPKHGSWLNMAEIEISALARQCLKRRLAS
                     EDELGREVAAWAEARNHGGATVDWRFTTDDARIKLKRLYPSL"
     gene            1990..2172
                     /locus_tag="AC159P_21955"
     CDS             1990..2172
                     /locus_tag="AC159P_21955"
                     /inference="COORDINATES: protein motif:HMM:NF024776.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR3698165.1"
                     /translation="MTTLTKRGSAPARVQTRARTLLLLHRGQTPDAVASALAVGVATV
                     FNVKRRYLGEGLTSAL"
     gene            2212..2295
                     /locus_tag="AC159P_21960"
                     /pseudo
     CDS             2212..2295
                     /locus_tag="AC159P_21960"
                     /inference="COORDINATES: protein motif:HMM:NF024954.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            2265..3059
                     /locus_tag="AC159P_21965"
                     /pseudo
     CDS             2265..3059
                     /locus_tag="AC159P_21965"
                     /inference="COORDINATES: protein motif:HMM:NF033545.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            3173..3625
                     /locus_tag="AC159P_21970"
     CDS             3173..3625
                     /locus_tag="AC159P_21970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3698166.1"
                     /translation="MSIGLRITLCLVALAFAGCGSTEVKPPTPGELKDFASVCDKANE
                     GKRIAVAGYLRFPDKFSGDRSVVLRVYQSADYAGKPVGVQAMVGKEANQVEAPPKQFT
                     DKDLKVHLTNGQVAGLGTKVKVSGDVYYPMVGQDFQCALSNPLVEPAN"
     gene            complement(4119..5384)
                     /gene="hutI"
                     /locus_tag="AC159P_21975"
     CDS             complement(4119..5384)
                     /gene="hutI"
                     /locus_tag="AC159P_21975"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014369199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - L-histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HIR3698167.1"
                     /translation="MTETLAVVNCSQVVTLAGPWRPRVGAEMRELGIVEGGALLVREG
                     LIERVGRREEVEPLLDADCEVFDAGGRVLTPGFVDAHAHPVFAGDRADEFEQRAAGAS
                     YQEIAAAGGGIRSTVRATRAASEDELFEIARRRRAWFLRTGTTTVEAKSGYGLSTADE
                     LKMLRVVRRLNEEGAVTYVPTFLGAHEVPDEYRARRAEYVELVVEEMLPRVAREGLAE
                     FCDVFCEERVFTVEESRRILSAARARGLALRLHADQLSLSGGARLAAELGAKTADHLE
                     HADAEGIAALRRAGVQPVLLPGSVYALGSKRYPAAREMIDAGLAVVLATDFNPGSSPT
                     PSMTMVLSIASTHMRVSPAEGITAATVNAAYSLNRGAETGTLEEGKRADFVVHDCADY
                     REPAYFFGVEHAHATFAAGRLVFARGGLK"
BASE COUNT         1011 a         1804 c         1675 g          920 t
ORIGIN      
        1 tgcgcatgga ggagatggcc gaggcctcgt tggcggcgcg gcgtgaggcg agcaggttgg
       61 ggatggcgat ggcggcgacg atgccgatga tcgcgacgac gatcagcagt tcgatcagcg
      121 agaagccttt ctggcctttt ttcgtttgca tttcttctgg gtgctcctta cggggatgtg
      181 tggtcaagaa aatttctctc gcgccgtcgg tcaacgtctc aggcttaacg tgccgcgcct
      241 tgcgcgaatc actcgtgctc cccttaaaca acccccatgc cacaaaaccg gcaaggcccg
      301 aaatcgccaa aagcccgctg tttccgccgt tccgaggccg ctcccgcgtc gggccgcgcg
      361 cccccgcccg aagacgaaag acggccacta acaaaattcg tcagccgccc tctgacgaaa
      421 aatgccaaaa ccgaaattgt ggaaattggc gggaaaagtc ctgaaaacgc ggctggacgg
      481 gttccagaaa gtaggcagta ggcggtaggc agtaggcagt taaagacaga agccagaagt
      541 cggaagtcag gagtcagaat aagcaagagg attgaaagct agttctttga cacagctaca
      601 acgatgggta gagccgcttg agcttgatgc gagcgtcgtc ggtcgtaaag cgccagtcca
      661 cggtggctcc gccgtggttg cgcgcctcgg cccacgccgc cacttctcgc cctaactcat
      721 cttcgctcgc cagccgccgc ttcaggcact ggcgcgcaag cgcgctgatc tcgatctcgg
      781 ccatgttgag ccaactgccg tgcttcggcg tgtaatgcaa ctccagcttg cgcgcgatgc
      841 gccgcgcctc ctcgggccta aagacttcat agagcgagga gggcgcgtgc gtgttcaggt
      901 tgtcgagcac caggacgatc cgctcggcct ccgggtagtg cacatccacc aactcgcgca
      961 tcacctcggc ccactgccgc gccccgcgct tcgccctgac ctcgacctcg cgcctcgcgg
     1021 ccagcggctc gaagagcatg aagaggttcg tcacgccccg gcgcacgtac tcgaagtcgc
     1081 agcgcacttc gcgcccaggc gcgaccggca gcggcagccg cgtctcgccg atcaactgca
     1141 cggccttctc gtccatgcag acctgcgggc gcaacggatc gaaggggcgc gtgtagacct
     1201 ccagcacatc ttccatcgcc gcgacgaagg cggcgttctg ttgcggcggg atgcaccact
     1261 gcttgacgag ccacggcttc agctcgtttt tttgagcacc cggcggacgg tctcgcgcga
     1321 gaccgcctcg accacgccca actcgacgag gcggccggtc aagagccgca ccgtccagcg
     1381 ggcacggcct gcgggcggct gggtgcaggc cagcgcgacc aggtgcgcct cggcctcgcc
     1441 gtcgagcttg tgcgggtaac ggcgaccggg gcggcgcgag tacagagccg tctcgaagct
     1501 ctcctcgacg aagcgctggc ggatgcgctc gaccgtggcg cgcccgacat cgagcgcctc
     1561 gacgatctgc tcgtcggtcc acccgatgcg accttcgccg cagtcggcct tgaggaggac
     1621 gcgggcgcgc gtcagcgtgc gggccggggt ctgacccgag cgcaccatct gctctaggct
     1681 gtgacgctct tccggggtga gcgtgatctg gtacttcttg ggcacggacg tcatcctcct
     1741 gaacaggggt cgaactgact tgcagaggat acaccggcac ccctcatccc agacgtgtca
     1801 aagaactagt gcaactccaa ggaagctgtt tggataggct cgacccgtga aatagtcggt
     1861 gcggtaacca tctgcctgta ttcgcctgga cactcgcaga ggtatcgccc tcatctatca
     1921 aaagaggtcg cttgatcttg cactaggaaa gcacagcccg tcagcttaac cgacgacgac
     1981 ctccgctctt tgacaacgct caccaagcgc ggcagcgcgc ccgccagagt gcagacccgc
     2041 gcccgcacgc tcttgctgct gcaccgcggc cagacaccgg acgccgtcgc ctcggcgctc
     2101 gcggtcggcg tggcgaccgt cttcaacgtc aagcgccgct acctgggcga aggactcacc
     2161 tctgcgctct aggataagcc gcgctccggc aagcccgccc gcatcgacgg cgtggcgcgg
     2221 gccaagatca cggcgctggc ctgctccgcc gcacccgagg ggcacgcccg ctggagcttg
     2281 cggctgctgg ccgacagggc cgtcgaactc ggcttggtcg aggccatctc acacaacacg
     2341 gtcaaggagg tgctgaaaaa aacgaactga agccgcacct caagcagacc tggtgcatcg
     2401 ggcagatcac gggcgagtac ctggcgcgga aggaggacgt gctgcacctt tacggcctcc
     2461 cctacaacga aaaacggcct ctggtctgct tcgacgaact gcccgtccaa ctcctgggcg
     2521 acgtggtcgc gccgctgggg atgaagccgg gcaagccgcg gcgcgaggac tacgagtacg
     2581 agaggggcgg ggtcgccgtg ctgctggtcg ccttcgagcc actgaccggc aggcggctgg
     2641 tcgagacgag caaacagcgg acgaaagctg actactgccg cttcatgcag agagtggccg
     2701 cgatgttccc cgccgccgag aaggtcatgt tggtgcagga caacctcaac acgcataacg
     2761 ccagctcttt ttatgagaac ctgccgccgg cggaagcctt cgcgctggcg caacgcttcg
     2821 agatgcatta cacgccgaag aaaggctcgt ggttgaacat ggcggaactg gagctgtcgg
     2881 ctatctcgcg catctgcctc tcgcggcgca tcgcctccat cgaggaactg gagcgtgagg
     2941 tgcaggcact tgtcaaagag cgcaacgagc tgaaggccaa agtcgagtgg cagttctccg
     3001 tctcgcaagc gcgagagaag ctgagccgcc actacgagaa cgcgaagtct aagaactaaa
     3061 gttacagagc actagggacg ttaggcagtt ctttattgct tcggactcgg agttaggata
     3121 aaaccctcaa gatttttctg acatcactta tcgactccgg gagaaccttt ccatgtctat
     3181 cggcctccgc atcacccttt gtttagtcgc cctcgcgttc gccggctgcg gcagcactga
     3241 ggtcaagccc ccgacccccg gcgagctgaa agacttcgcc tcggtctgcg ataaagctaa
     3301 cgagggaaag cgcatcgccg tggcgggcta tctgcgcttc cccgataagt tcagcggaga
     3361 ccgcagcgtg gtactcaggg tataccagtc ggcagactac gccggtaaac cagtcggggt
     3421 gcaggccatg gtaggtaaag aggccaacca ggtggaagcg cccccgaagc agttcaccga
     3481 caaagacctc aaggtacatc tcacgaacgg gcaggtagcc ggactcggga cgaaggttaa
     3541 agtctccggt gatgtctatt acccgatggt ggggcaggat tttcagtgtg cgctgagtaa
     3601 cccgctagtc gagccggcca actgacgagc cccctccccc gcgggggccc tgtgcctccc
     3661 cataacaggg ccactcttcg agcaaggctg ccactactac ttgcggcatc aagaagctgg
     3721 cccctccttg caggagagca gagcgccatc agtaagaggg actgagtcac ttcaattaac
     3781 gtggcaccat cccctaaaaa ccagaagggc cgtcgcgtct cgcaacggcc ccggtgggtc
     3841 tgtttggttt aaccggcccc cgcagtcagt ctgacttaaa tctggtgtgt cgctggtgtg
     3901 tcacctcttc cgaaaacccc aacctatgtc accaccgagg agtcgcagcg gcgaaaagcc
     3961 ggggctttag cgacagcttg tatgggttag ccggtttcag ccacctcact gtgataagaa
     4021 ctcatacgct ttggatcacc tcctttccag tgttgggcgc aatatagacg acaataactt
     4081 gtgcgtcaat cattattttc acccactatc ttgtggcttt actttaagcc gccgcgcgcg
     4141 aaaacgagcc tccccgccgc gaacgtcgcg tgcgcgtgct cgacgccgaa gaagtaggcc
     4201 ggctcgcggt agtcggcgca gtcgtggacg acgaagtccg cccgcttccc ctcttccagc
     4261 gtccccgtct cggccccgcg gttcaggctg taggcggcgt tgacggtcgc cgccgtgatt
     4321 ccttcggcgg gcgacactct catgtgcgtc gaggcgatgg agagcaccat cgtcatcgac
     4381 ggcgtgggcg acgagccggg gttgaagtct gtggcgagca cgacggcgag tcccgcgtcg
     4441 atcatctcgc gcgccgccgg atacctcttc gagccgaggg cgtagaccga gccgggcagc
     4501 agcaccggct gcacgcccgc gcgtcggagc gccgcgatgc cttccgcgtc cgcgtgttcg
     4561 aggtgatccg cggtcttcgc gcccagctcg gcggcgagcc gcgcgccgcc cgacagcgac
     4621 aactgatccg cgtgcaggcg caaagccagc ccgcgcgcgc gcgccgccga gagtatccgc
     4681 cgcgactctt cgaccgtgaa gacgcgctcc tcgcagaaca catcgcagaa ctcggccagc
     4741 ccctcgcgcg cgacgcgcgg cagcatctct tcgacgacca gctcgacata ctccgcgcgg
     4801 cgcgcgcggt actcgtccgg cacctcgtgc gcgccgagga aggtcggcac gtatgtgacc
     4861 gcgccctcct cgttcaggcg gcgcacgacg cgcagcatct tcagctcgtc tgcggtcgaa
     4921 agcccgtagc cggacttcgc ctcgaccgtg gtcgtgcccg tacgcaggaa ccaggcccgg
     4981 cgccgccgcg cgatctcgaa gagctcatct tccgaggcgg cccgcgtcgc gcgcacggtc
     5041 gagcggatgc cgccgcccgc cgccgcgatc tcctgatagc tcgcgcccgc cgcgcgctgc
     5101 tcgaactcgt cggcgcggtc gcccgcgaag acggggtgcg cgtgcgcgtc cacgaagccc
     5161 ggcgtcagga cgcgcccgcc cgcgtcgaag acctcgcagt ccgcgtccaa cagcggctcg
     5221 acctcttcgc gcctgccgac cctttcgatc agcccttcgc ggacgagcag cgcgccgccc
     5281 tcgacgatcc cgagctcgcg catctccgcc cccacgcgcg ggcgccacgg ccccgcgagc
     5341 gtgacgacct gcgaacagtt gacgacggcc agcgtctctg tcattcgctc actccgggtg
     5401 aagagggtgc
//
LOCUS       DBNGOH010001129         2439 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2005702, whole genome shotgun sequence.
ACCESSION   DBNGOH010001129 DBNGOH010000000
VERSION     DBNGOH010001129.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2439)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2439)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2439
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2005702"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..851
                     /locus_tag="AC159P_21980"
     CDS             <1..851
                     /locus_tag="AC159P_21980"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005456733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HIR3698168.1"
                     /translation="FQRLRRMGADGTPVSKRVFGEGTHLRPSIIQFFRAERVLLEDYT
                     VLNSPFWVNHLVYTDHATVRRVRVDSHRANNDGVDVDSSRHVLIEHCWFRTGDDSVVV
                     KSGRDRDAREIGRPSEHVVVRHNDMGGEDGIALGSEMSGGIRHVYFTDNVLRKGASAI
                     RFKANLDRGGTVEHVRVRNFTVEAFDNLIWFQLDYPGQLGGNFPSTYRDIVFENFTVE
                     RAGTVFNGDAPAAAPLRDVTLRNIDIKQADRTFVLKNVEGLKLENVRIGGQRVDGRLD
                     WRQAAP"
     gene            978..2135
                     /locus_tag="AC159P_21985"
     CDS             978..2135
                     /locus_tag="AC159P_21985"
                     /inference="COORDINATES: protein motif:HMM:NF013434.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR3698169.1"
                     /translation="MNAERTYDTAVIGAGVFGAWTAYRLRESGQSVALLDGYDPAHSR
                     ASSGGESRIIRMGYGADELYTRWAARSLLLWQDFFRQVGRPLFQRTGVLWLARADDPY
                     TLATLETLAEVGVKFERLTLAELEGRFPQFTLGPDAWGVFEPDSGVILARGAVREVVR
                     EAVGRGVTYLAEAVVAPEGRGRLDSVKTRSGLKLAAANFVFACGPWLPKIFPRLLGEW
                     IRPTRQEVFFFGVEAGDPAFTTPAMPAWVDFRELVYGVPDLEGRGFKLAIDAHGPPFD
                     PDAGERLATAEGLALARAHLARLLPGLRDAPVVETRVCQYENTPHGDFLIDRHPDFEN
                     VWLVGGGSGHGFKHGPALGEYVAGKVTQADTDSEPRFVLCAGGKARQRLVY"
     gene            2139..2342
                     /locus_tag="AC159P_21990"
     CDS             2139..2342
                     /locus_tag="AC159P_21990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3698170.1"
                     /translation="MGRAFPGSGLPFGAAFRLVADETAVPLLGLSKGPTEYPPSVHAY
                     ALASHFVYGLTAEVVRRAVRRVL"
BASE COUNT          380 a          872 c          808 g          379 t
ORIGIN      
        1 acttccaacg cctgcgccgg atgggcgcgg acggcacgcc cgtctccaaa cgcgtcttcg
       61 gcgagggcac gcacctcagg ccgagcatca tccagttctt ccgcgccgag cgcgtgctgc
      121 tcgaagatta cacggtactg aactcgccgt tctgggtgaa ccacctcgtc tacacagacc
      181 acgccaccgt gcggcgcgtc cgcgtggaca gccaccgcgc caacaacgac ggcgtggacg
      241 tggattcgag ccgccacgtt ctgatcgagc actgctggtt ccgcacgggc gacgactcgg
      301 tggtggtgaa gtcggggcgc gaccgcgacg cccgcgagat cgggcgaccg agcgagcatg
      361 tcgtcgtccg ccacaacgac atgggcggcg aggacggcat cgccctgggg agcgagatgt
      421 ccggcggcat ccggcacgtc tatttcaccg acaacgtgct gcgcaaaggg gcttcggcca
      481 tccgcttcaa ggccaacctc gaccgcggcg gcacggtcga acacgtccgc gtccgcaact
      541 tcacggtcga ggcgttcgac aacctcatct ggttccagct cgactacccc ggccagctcg
      601 gcggcaactt cccttccacc taccgcgaca tcgtcttcga gaacttcacg gtcgagcgcg
      661 ccggcacggt cttcaacggc gacgcgcccg ccgccgcgcc gctgcgcgac gtgacgctgc
      721 gcaacatcga catcaagcag gccgaccgca cgttcgtcct caagaacgtc gaagggctga
      781 agctggaaaa cgtccgcatc ggcggccagc gcgtggacgg acggctcgac tggcggcagg
      841 ccgcgccatg accatgagtt tgccgtgcag gcccgtgagc tggtgacgca cgaattcgtc
      901 ccccgccgag cgcggcgccc cgacgccggg aacgccgaac caagctctcc ctttgcgaga
      961 gccggcggcg acatttcatg aacgcagaac ggacgtacga caccgccgtt atcggcgccg
     1021 gagtcttcgg cgcctggact gcctaccgcc tacgggaatc cggccagtcc gtcgcgctgc
     1081 tcgacggcta cgacccggct cacagccgcg ccagctccgg cggcgaatcg cgcatcatcc
     1141 gcatgggcta cggggccgac gagctttaca cgcgctgggc ggcgcgctcg ctcttgctct
     1201 ggcaggattt cttccgtcag gtgggccggc ccctcttcca acgcacgggt gtcctctggc
     1261 tcgcgcgggc cgacgacccc tacacgctcg ccaccctcga aacactcgcc gaggtcggcg
     1321 tcaagttcga gaggctgacg ctggcggagc tggaaggtcg cttccctcaa ttcaccctcg
     1381 gcccggacgc gtggggcgtc ttcgagcctg acagcggggt catcctggcg cgcggcgccg
     1441 tccgagaggt cgtccgcgag gccgtcggac gcggcgtcac ctacctcgcg gaagccgtcg
     1501 tcgcgcccga aggtcggggg cgactcgact ccgtcaaaac gcggagcgga ctcaagctcg
     1561 ccgccgcgaa cttcgtcttc gcctgcggcc cctggctgcc gaaaatcttc ccccgtctgc
     1621 tgggggagtg gatcaggccg acgaggcagg aggtcttctt cttcggcgtc gaggcgggcg
     1681 accctgcctt caccacgcct gccatgcccg cctgggtcga tttcagagag ctggtctacg
     1741 gcgtccccga ccttgagggc cggggcttca agctcgcgat tgacgcccac ggcccgccct
     1801 tcgacccgga cgcgggcgag cggctggcga cagccgaagg gctggcgtta gcgcgcgcgc
     1861 acctggcccg cctcctgccc ggtctgcgag acgcgccggt cgtcgagacg cgcgtctgcc
     1921 agtacgagaa caccccgcac ggcgacttcc tgatcgaccg ccaccccgac ttcgaaaacg
     1981 tctggctcgt cggcggcggc tcggggcacg gcttcaagca cggcccggcg ctcggcgagt
     2041 acgtcgccgg caaggtcacg caggcagaca ccgactcgga gccgcgcttc gttctctgcg
     2101 ccgggggcaa ggcccggcag cgcctggtgt actgaacttt gggccgagca tttccggggt
     2161 cggggctgcc gttcggcgcg gccttccggc tcgtggcgga cgagacggcg gtgccgctgc
     2221 tcggcctctc gaaagggccg accgagtatc ctccctcggt gcacgcctac gcgctcgcgt
     2281 cgcacttcgt ttacggtctg acggcggagg tcgtgcggcg cgcggtgcgc agggtgctgt
     2341 aagtagtccg cactttgccg ggaaggaagc gttgaaagaa ctgtaatacc gccttgcgaa
     2401 tcagactgcc gccgccgcag gcggtggccg ccatcacgg
//
LOCUS       DBNGOH010001130         3732 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3838434, whole genome shotgun sequence.
ACCESSION   DBNGOH010001130 DBNGOH010000000
VERSION     DBNGOH010001130.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3732)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3732)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3732
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3838434"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..604
                     /locus_tag="AC159P_21995"
     CDS             <1..604
                     /locus_tag="AC159P_21995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3698171.1"
                     /translation="EFARAVVPNADKLKATQEIFRPGMTLASLSRSHNIGGEAVAQSG
                     RILYANGVFFANRPVLVNGIMLADRGVVLGDGLIAADGVLVGNGLLLSDGLIAADRGV
                     VLGDGLIAADGLICADGLIAADGLIAADGLISADGLIAADRGVVLGDGLIAADGLISA
                     DGLIAADGLIAADGLISADGLIAADRGVVLGDGILIGEAE"
     gene            complement(928..3036)
                     /locus_tag="AC159P_22000"
     CDS             complement(928..3036)
                     /locus_tag="AC159P_22000"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF012630.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031012 - extracellular matrix [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HIR3698172.1"
                     /translation="MRLPVTLLVAAALVCGLAPPPARAYTLQLNSAATVQFRWPTTTI
                     TIALSSSLNSPPANIKAGSDVVGAARRALQRWAEAGNINFNITTSTETRVQQDGVSLI
                     TVSPTSGVTFTNPGQTGRARVFFDASSGAITEGDVAVNPATQFSTDGTPDTFDLETTF
                     AHEIGHVLGLEHSGLAGALMQPRQTRNGTFDLAAIRRRVLSDDDRAGLRAIYGPLSGL
                     GELRGTVRNAAGGPVFGAHVVAEHAATGKAVAGNITLSDGRFLIRSLPPGQYRVVVEP
                     LNEPVLASEIATRSGTAYAGLQGVLPDFLTFERTSNTVTVAEGAPASLEVTVNSQQPF
                     LNPRYVGVNSQLTTSAAPVVPGSTFNLLVGGENLHTVGAGGVSITSPYFTINQSSFQQ
                     LTFQGPNGLVQVLSFDVTASLVTAPGEYSVRLLSGAGEVGYYTSGLTVDLPNGVTGGP
                     NLIDRTQFFVAQHYRDFLNREPDAGGLDFWSNAIERCGADAQCREVERVNVSAAFFFA
                     IEFQETGYLVYRSHQAAFGTGERLRFNAFLRDTQQIGRGVEVGVGDWQARLEANKAAF
                     FGEFVNRPEFLALYPPTLSPAQFVDALNANTGGSLTQAERDTLVADLAAGRRTRAQVL
                     RAVADDPEFRQREFNKAFVLMQYFGYLRRNPDDPPDASFEGFDFWLQKLNQFGGDFRA
                     AEMVRAFINSIEYRARFGPS"
     gene            complement(3261..3563)
                     /locus_tag="AC159P_22005"
     CDS             complement(3261..3563)
                     /locus_tag="AC159P_22005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3698173.1"
                     /translation="MSRNVARPTARARRKGRNALLWVGLSALVIILLLYFEQIALLYV
                     LATLSVAALLMIVAFADLGGARRVSSEPAPRDDSAAIGDGTTVATSFGTAASRQRR"
BASE COUNT          599 a         1205 c         1326 g          602 t
ORIGIN      
        1 cgagttcgcg cgcgccgtcg tcccgaacgc cgacaagctg aaggccacgc aggaaatctt
       61 ccgccccggc atgacgctcg cttcgctcag ccgctcgcac aacatcggcg gcgaggcggt
      121 ggcgcagtcg ggccgcatcc tctacgccaa cggcgtcttc ttcgccaacc gcccggtgtt
      181 ggtgaacggc atcatgttgg ctgaccgcgg cgtcgtcctc ggcgacgggc tgatcgccgc
      241 cgacggcgtg ctggtcggca acggcctgct gctttcggac ggtctcatcg ccgccgaccg
      301 cggcgtcgtc ctcggcgacg ggctgatcgc cgccgacggt ctgatctgcg ccgacggcct
      361 gatcgccgcc gacggtctga ttgccgccga tggcctgatc tcagccgacg gcttgatcgc
      421 cgccgaccgc ggcgtcgtcc tcggcgacgg gctgatcgcc gccgacggtc tgatctccgc
      481 tgacggcctg atcgccgccg acgggctgat tgccgccgac ggcctcatct cagccgacgg
      541 tctcatcgcc gccgaccgcg gcgtcgtcct cggcgacggc atcctgatcg gcgaggccga
      601 gtaacatcgt cgaggccgga gttcgatgaa gggcgtcacg cccggataga agccgactcg
      661 gcaagcgaca tagcgaaggg cgggtggaga agccttcacg gcaactctgc ccgcccttcc
      721 tcctgggtga gtgagcagta agcagcgagc agtggaagac agcaggcgag agtcggaagg
      781 gcgaagaggc ttgggaggta aagtccacag catcttcgcc cttcgagtaa taacacaaga
      841 ggcttgggac tttacctccc aagcctcttc gcccttccgt ccttactgcg taccgcctac
      901 tgtctttccc cgctcaccgc tcactcctta tgacgggccg aagcgggcgc ggtattcgat
      961 ggagttgatg aaggcgcgca ccatctccgc ggcgcggaag tcgccgccga actggttgag
     1021 cttctgaagc cagaagtcga agccctcgaa gctcgcgtcg ggcgggtcgt ccgggttgcg
     1081 cctgagatac ccgaagtatt gcatcagcac gaacgccttg ttgaactcgc gctgccggaa
     1141 ctccgggtcg tccgcgacgg cgcgcaagac ctgcgcgcgc gtgcgccggc ccgcggcgag
     1201 gtccgcgacg agcgtgtcgc gctcggcctg tgtgagcgag ccgccggtgt tggcgttcag
     1261 cgcgtccacg aactgcgcgg gcgagagcgt gggcgggtag agcgcgagga attcggggcg
     1321 gttgacgaac tcgccaaaga aggccgcctt gttcgcttcg agccgcgcct gccagtcgcc
     1381 gacgccgacc tcgacgccgc gcccgatctg ttgcgtgtcg cgcaggaacg cgttgaagcg
     1441 cagccgctcg cccgtgccga aggccgcctg gtgcgagcgg tagacgagat agccggtctc
     1501 ctgaaactcg atggcgaaga agaaggccgc cgagacgttg acacgctcca cttccctgca
     1561 ctgcgcgtcc gcgccgcacc gctcgatggc gttcgaccag aagtcgaggc cgcctgcgtc
     1621 cggctcgcgg ttgaggaagt cgcggtagtg ctgcgcgacg aagaactgcg tccggtcgat
     1681 caggttcggc ccgcccgtca cgccgttcgg cagatcgacc gtcaggcccg aagtgtagta
     1741 accgacctcg cccgcgccgc tcaagaggcg cacgctgtac tcgcccggcg cggtgacgag
     1801 gctcgccgtc acgtcgaagc tcaagacctg caccagcccg ttcggcccct ggaaggtgag
     1861 ctgctgaaag ctcgactggt taatggtgaa atagggcgag gtgatgctga ctccgcccgc
     1921 gccgaccgtg tgcaggttct cgccgccgac cagcaggttg aacgtcgagc cggggacgac
     1981 gggcgcggcg gaggtcgtga gctgcgagtt gacgccgacg tagcgcgggt tgaggaacgg
     2041 ctgctgggag ttcaccgtca cttcgagcga ggcgggagcg ccctccgcga cggtgaccgt
     2101 gttcgaggtg cgctcgaagg tgaggaagtc gggcagcacg ccctgtagcc ccgcgtaggc
     2161 cgtgccgctg cgcgtcgcga tctcggaggc gagcaccggc tcgttgagcg gctcgacgac
     2221 gacgcggtac tggccgggcg gcagcgagcg gatgaggaac ctgccgtccg agagcgtgat
     2281 gttacccgcg acggccttac cggtcgccgc gtgctcggcc accacgtgcg cgccgaagac
     2341 ggggccgccc gcggcgttgc gcaccgtgcc gcgcagctcg ccgagtcccg acagcgggcc
     2401 gtagatggcg cgaaggcccg cgcggtcgtc gtccgagagc acgcggcggc ggatggccgc
     2461 gagatcgaac gtgccgttgc gcgtctgccg cggctgcatg agcgcgcccg cgaggcccga
     2521 gtgttcgagg ccgaggacgt ggccgatctc gtgcgcgaag gtggtctcca aatcgaaggt
     2581 gtcgggcgtg ccgtcggtcg agaactgcgt cgcggggttg acggccacgt cgccctccgt
     2641 gatcgcgccc gacgaggcgt cgaagaagac gcgcgcgcgg cccgtctgtc cggggttggt
     2701 gaacgtgacg ccgctcgtgg gcgagacggt gatgaggctg acgccgtcct gctgcacgcg
     2761 cgtctcggtc gaggtcgtga tgttgaagtt gatgttgccc gcctcggccc agcgctggag
     2821 cgcgcgccgc gccgcgccga ccacgtcgct gcccgccttg atgttcgcgg gcggcgagtt
     2881 gagcgaggag gagagggcga tggtgatggt cgtcgtcggc cagcggaact ggacggtcgc
     2941 cgcgctgttg agttgcagcg tgtaggcgcg cgcgggcggc ggcgcgaggc cgcacacgag
     3001 cgcggcggcg acgaggagcg tgactggcag acgcataccg tgaagaccct cttgattgat
     3061 tgtcacgtac cgaggaacgt tcggtgggga gcttgatccg cgcttcaggc ggggccttca
     3121 cactatagca agacgcggcg cgccgcttca aacatttaat tcagggggga aaaggttaaa
     3181 aggggaagag gcgaaaaggg agaaacagtt ctttcccttt tcgactcttc cccttttaac
     3241 cttttccccc ggtctgcccg ttagcggcgc tggcggctgg cggcggtgcc gaaggaggtg
     3301 gcgacggtcg tgccgtcgcc gatggccgcc gagtcgtcgc ggggcgcggg ttcgctcgac
     3361 acccgacgcg cgccgcccag gtccgcgaac gcgacgatca tgagcagcgc ggcgacgctc
     3421 agcgtggcga gcacgtagag cagcgcgatc tgctcgaagt agagcaggag gatgatgacg
     3481 agcgcgctca acccgaccca gagcagcgcg ttgcggccct tcctgcgcgc gcgggccgtc
     3541 ggcctggcga cgtttcgact cataataaat tctccttgaa aaagtatcgt caagcggaaa
     3601 gggttaattt aacacaggcg ccgcgcgggc ggcgaacggc ggcgagtttc ggcagcggcc
     3661 atttcatcga aatcggctca cgaaaaaggg gctggccacg cgtccggcca gcccctccgg
     3721 ctttcgtctt tc
//
LOCUS       DBNGOH010001131         3294 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1273558, whole genome shotgun sequence.
ACCESSION   DBNGOH010001131 DBNGOH010000000
VERSION     DBNGOH010001131.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3294)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3294)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3294
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1273558"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(27..611)
                     /locus_tag="AC159P_22010"
     CDS             complement(27..611)
                     /locus_tag="AC159P_22010"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013579231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase family protein"
                     /protein_id="HIR3698174.1"
                     /translation="MARRGGTWVQRWRVPLGFLCAGVFLLLARPRPLTLAVGGAVALV
                     GLSIRAWAAGHIRKNAALAVSGPYAHTRNPLYLGSLVMGLGFTVAAAGTWWLFVLFAG
                     LFAALFLGIYLPVMRVESATLAELFGDEYEVYAREVPLLFPRLTPYRKGDNSEKTKFD
                     AGLYLRYREYRAALGSLLAWCVLAIKAVLGVLAG"
     gene            complement(601..1707)
                     /locus_tag="AC159P_22015"
     CDS             complement(601..1707)
                     /locus_tag="AC159P_22015"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HIR3698175.1"
                     /translation="MILFVKLGSIGDIVHTLPALAAVRGAVPGAEVSWVVERRAAEIL
                     RDNRLLKGLIEVDTKALRRVPMSGETLLAPRQQLRRLRASPFDLSLDFQGLLKSAAIA
                     RLSGARRRYGFGKAALREPASRFLLTETINVPARTHVIRKNLALAAGALGIDVPEDGR
                     DFEFPIDVSLEHEREAERIAEQAGEDFAILNPGGGWPTKLWGAERFGSLADALWSSYG
                     LRPIVTYGPGELGLAEEVARASRSGRTLLAGPSLKGFYALARRARLYVGGDTGPTHLA
                     VAASAPVVGLFGPTEWWRNGSPRAEDICVERNDIDCRTDCHRRACSRWVCMDIEVGRV
                     LEAAGERLRRASKASRLAKVSGAAGMSGALTHGA"
     gene            complement(1807..2313)
                     /locus_tag="AC159P_22020"
     CDS             complement(1807..2313)
                     /locus_tag="AC159P_22020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011554696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HIR3698176.1"
                     /translation="MNFDEQQKAEAAARILERNRVIARVAIARRNGARVVFANGCFDL
                     LHVGHVRYLEAARALGDLLIVGVNGDAQVRALKGEGRPFVPERERAEVIAALRSVDLV
                     TIFHEPTVEALLLAVRPDIHAKGTDYTEETVPEREAVRSYGGRVAIVGDPKEHSSTEM
                     LKAVNREP"
     gene            complement(2350..>3294)
                     /locus_tag="AC159P_22025"
     CDS             complement(2350..>3294)
                     /locus_tag="AC159P_22025"
                     /inference="COORDINATES: protein motif:HMM:NF024136.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3810 domain-containing protein"
                     /protein_id="HIR3698177.1"
                     /translation="LAALAAWLAWHVHQLVRRRQSVRGLLASAFVRVCWVASVLAAVF
                     MPLFGLNYLRPPLAETLRLEQREPDAREAETISRAVIEGVNRNFAESGAAATAEEGSR
                     LPLTRPELFAVLEESFAAERLLQGVSAGGAAAAPKPVYFSGLLSRLGTSGIYSPFTGE
                     PHYNAIMPDADLPFTVAHEMAHQRGFARESEASFVAFLVCTKSSHPYVRYSGYLGALR
                     VLSVLRRLAPERYAPVAALLGDGPRADLRAGDAFWSRYGGRLARVSRRVNNAYLRANR
                     VRSGVRNYGEVVALVVGYYLTHGLAPQPVADSAARSYP"
BASE COUNT          567 a         1116 c         1148 g          463 t
ORIGIN      
        1 tagtgaaaac ccttcttttc gtattatcac ccggcgagaa cacccagaac tgccttgatc
       61 gccagcacgc accatgccag aagcgaaccg agcgcggccc tgtattcgcg ataacgaaga
      121 tacaggccgg cgtcgaactt cgtcttctcc gaattgtcac ccttccggta aggcgtcagc
      181 cgcgggaaga ggagcggcac ctcgcgcgcg taaacttcgt actcgtcgcc gaacagctcg
      241 gccagtgtcg cggattcgac gcgcatcacc ggcagataga tgcccaggaa aagcgccgcg
      301 aacagccccg cgaagagcac gaacagccac cacgtccccg ccgccgcgac cgtgaagccg
      361 aggcccatca ccaggctccc caggtagagc gggttgcgcg tgtgcgcgta ggggccggag
      421 acggccagcg cggcgttctt gcggatgtgc ccggcggccc acgcgcgtat cgagaggccg
      481 acgagcgcga ccgcgccgcc gacggcgagc gtcaaggggc gcggtcgcgc gagcagcagg
      541 aagacgcccg cgcacagaaa gccgagcggc acgcgccagc gctgcaccca cgtcccgccc
      601 ctacgcgcca tgcgtcaatg cccccgacat gccggccgcc cctgacactt tcgccaagcg
      661 cgaagccttc gacgcacgcc gcaggcgctc gcccgccgct tcgagcacgc gcccgacctc
      721 gatatccatg cagacccagc gcgagcacgc gcggcggtga cagtccgtgc ggcaatcgat
      781 gtcgttacgc tcgacgcaga tgtcttcggc gcgcgggctg ccgttgcgcc accactcggt
      841 cgggccgaac agcccgacca ccggcgcgct ggccgcgacc gccagatggg tcggccccgt
      901 gtcgccgccg acgtacaggc gcgcgcgccg cgcgagcgca taaaaacctt tgaggctcgg
      961 gccggcgaga agcgtcctgc ccgaacggct cgcgcgcgcg acctcttcgg ccagcccaag
     1021 ctcgccgggg ccgtaggtga cgattgggcg caagccgtag ctcgaccaca acgcgtccgc
     1081 gagcgagccg aagcgctcgg cgccccacag cttcgtcggc cagcccccgc cggggttcag
     1141 gatggcgaag tcttcgcccg cctgctccgc gattcgttcg gcctcgcgct cgtgttcgag
     1201 agacacgtcg atggggaact cgaagtcgcg accgtcttcg ggcacgtcga tgccgagcgc
     1261 cccggcggcg agcgcgaggt tcttcctgat aacgtgcgtg cgcgcgggga cgttaatcgt
     1321 ctccgtcaag agaaagcggc tcgccggctc gcgcagcgca gccttgccga aaccgtagcg
     1381 gcggcgcgca cccgagagcc gggcgatggc ggcggatttt agaagtccct ggaaatctaa
     1441 agataagtcg aagggcgagg cgcgtaaccg acgcaactgc tgacgcggtg ccagcagcgt
     1501 ctccccagac atcggcacgc gcctcagcgc cttcgtgtcc acttcgatga gacctttcag
     1561 cagacggttg tcccgcaaaa tttccgccgc ccgccgctcc accacccacg acacctccgc
     1621 ccccggcaca gccccccgca ccgccgccaa cgccggcagc gtgtgcacga tgtcgccgat
     1681 gctcccgagt ttgacgaaaa gaatcatcat gcaaatgccg tgaaccgtga accgtgaacc
     1741 gtgacaagta aaaagccgtg aaccgtgaac cgtaaaccgt gacaggtgaa aaccgattgc
     1801 ttcctgtcac ggttcacggt tcacggcttt taacatttcc gtgctcgaat gctccttcgg
     1861 gtcgccgacg atggcgacgc gcccgccgta ggagcggacg gcttcgcgct cggggacggt
     1921 ctcctctgtg tagtctgtgc ccttggcgtg gatgtcgggc cggacggcga gcagcagcgc
     1981 ctcgaccgtc ggctcgtgga agatggtgac gagatcgacc gagcgcaacg cggcgatcac
     2041 ttcggcgcgc tcgcgctcgg ggacgaaggg gcggccctcg cccttgaggg cgcgcacctg
     2101 cgcgtcgccg ttgacgccga cgatgagcag gtcgccgagc gcgcgcgccg cttccagata
     2161 gcgtacatgc ccgacgtgca ggaggtcgaa gcagccgttg gcgaagacga cgcgcgcgcc
     2221 gttcctgcgc gcgatggcga cgcgcgcgat gacgcggttg cgttcgagga tgcgcgcggc
     2281 ggcttccgcc ttctgttgtt cgtcgaagtt catttccgtg gttcgtcgaa agtggggtgg
     2341 cgagcgtctt caaggataag agcgcgcggc ggaatccgca accggctgcg gcgcgagtcc
     2401 gtgggtgagg taatagccga cgacgagcgc gacgacctcg ccgtagttgc gcacgcccga
     2461 gcggacgcgg ttggcgcgca ggtaggcgtt gttgacgcgg cgcgacacgc gggcgagtct
     2521 gccgccgtag cggctccaaa aggcgtcgcc cgcgcggagg tctgcgcgcg ggccgtcgcc
     2581 gaggagggcg gcgacgggcg cgtagcgttc gggcgcgagg cggcgcagga cggagaggac
     2641 gcggagcgcg ccgaggtagc cggagtagcg gacgtagggg tgcgaggact tggtgcagac
     2701 gaggaaggcg acgaagctcg cctcggactc gcgcgcgaag ccgcgctggt gcgccatctc
     2761 gtgcgcgacg gtgaagggca ggtcggcgtc gggcatgatg gcgttgtagt gcggctcgcc
     2821 cgtgaagggc gagtagatgc ccgacgtgcc gaggcggctc agcagccccg agaagtagac
     2881 aggcttcggc gccgccgccg cgcccccggc gctcacccct tgaaggagtc gctccgcggc
     2941 gaaggactct tcgaggacgg cgaagagttc ggggcgggtc agagggaggc ggctgccctc
     3001 ttcggcggtc gccgccgcgc cgctctccgc gaagttgcgg ttgacgccct cgatgacggc
     3061 gcggctgatg gtctcggcct cgcgcgcgtc cggctccctc tgttcgagcc gcagggtttc
     3121 ggccagcggc gggcgcaggt agttcagccc gaacaagggc atgaagacgg cggcgagaac
     3181 cgacgccacc cagcacaccc gcacgaaggc ggacgctaaa aggccgcgca cgctctgacg
     3241 gcgacggacg agctgatgga cgtgccacgc gagccacgcg gcgagtgcgg cgag
//
LOCUS       DBNGOH010001132         6363 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2557099, whole genome shotgun sequence.
ACCESSION   DBNGOH010001132 DBNGOH010000000
VERSION     DBNGOH010001132.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6363)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6363)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6363
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2557099"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            71..2899
                     /locus_tag="AC159P_22030"
     CDS             71..2899
                     /locus_tag="AC159P_22030"
                     /inference="COORDINATES: protein motif:HMM:NF012801.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR3698178.1"
                     /translation="MARLLIRAAIATVWLALCHVALAAQAVPAGATLDGTVTDQHGAV
                     VVGAEVRALNIASGRTLAARTDADGRYELTGLAPGPYRLSVSAAGFTAAARRVRVERA
                     PDPVSEDFVLVPGIVESSVTVTAGKGSARAVAETPQSVTVSDAARIEGRRPASTLQAV
                     EQAPNLTPVIANPALERPRLRGLASNRLLVILDGERLNNIRSDPTSGVSPSVVDVTQL
                     ESAEVLGGAGSSLYGSDAMSGVINLITRGPVSAGDGDPVLGFRFDGDARTNGRFRRGA
                     GVVNASVPRLAFRFGGSLFRAGDYDAGDRAIPLEDVVRLGNLATEMSNAGGGGVARTY
                     AVWSLPAGARVPNGQARGFNDQVDSWFFPRANHGLRYRQLNSQHKNLGFSFIAPPFDQ
                     RTQFNAFRRLDKYGLRYEARELLPWLPRLAVGFYRQKYSFPDDTITSPIDADSSWTLV
                     PNPDDPQTPFAVLTGRPSTFTDAGFTDNKNSITTWAFDAQATFAPFTGTLFTTGVGYL
                     RDSSADEFSRVEFVPGTRDVRTSISGRASNPDSVYRNWGWFNLLEYEPVRWLRLVGGL
                     RVDNWRTEARATRGFPLGNEAAILDASFAQLLANPGQINTDGAAGIADLLDGDGSINT
                     SRTVTTGNVGVVFRLPHGVNPYLRWGNSYREPGITERYLLRDFGDPAFSVLVVPNASL
                     KPERGESFDLGVKVQRARLFASAGYFRNRLEDFIGNAFAAPVFVPADPARGLQPLSPF
                     FPFHGVLYVQRTNTARARIEGFEAVYELSLPLGERGVLTPFGALGWLKGANLTPDETT
                     LALIERFYNRDDTPIPLRGSAEDAPLTGITPFRMVGGVRYDSFRRRWFGEFELRYQGR
                     VRRVDPLDLTTAISTQYGSLAGLDSFARHTLRAGYTLRRESYRAAFTLGVENLTDRLY
                     FEHFQTAPAPGRSVVFGLTLDLFDLLRK"
     gene            2950..3672
                     /locus_tag="AC159P_22035"
     CDS             2950..3672
                     /locus_tag="AC159P_22035"
                     /inference="COORDINATES: protein motif:HMM:NF020893.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HIR3698179.1"
                     /translation="MKRYLLAACAVVFVMTILFAAVEALGVPLLTNPAPWMKGGGALA
                     ACVGVGLLVADVLLPVPSSLVMVAHGALFGVAGGTLLSLAGSLGAALVGCWIGRRGGR
                     LLERAVKTVKPDEAARADYLLRRWGALAIIVTRPVPLLAETVALLAGASRMSWGRVAA
                     AALLGSLPPALLYALTGAAVANLQSTALMFVLVILVAGFFWMLGRLLDPFRRRGEGTR
                     RFRDASSFPSLEPQRKENSHGD"
     gene            3662..4894
                     /locus_tag="AC159P_22040"
     CDS             3662..4894
                     /locus_tag="AC159P_22040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3698180.1"
                     /translation="MAIKLTINGSPGPEGFLVAPAGRQTFPAIVGLKTDDGTTVQAKL
                     QVVAPKGVVVVVSPTTLSIGPTQKTVKLTAKTPSKSQGDISFQVKVGLTVKAKLKLTC
                     VTDPRLRFGGRFQARFATDGDFFNNARGTSAGWTWALEGEPDFVPAANNVPTAPGMAV
                     GRVVRFQNAVAPRPLVAPIGVTVTAVEATLGTKTVSFTKGDAVIGQKVELGPNSYLAS
                     NRPANPALPPPFENYGPGFEPIENFVFEVGALFSGKPASLNDRPKANGFFPLTPTQLT
                     QYGIVALSTFNTQRKNALLAAYNALSPADRTGTPAGRNLATRISHLGGLPAHSIPPTQ
                     ATLSLGWSGQETYNGLVNSAIMITATKSPVLAYLKTFQSFRYSGMFFNFHSDELCGRV
                     DGTLRAMTLALSRLPPQE"
     gene            5034..>6363
                     /locus_tag="AC159P_22045"
     CDS             5034..>6363
                     /locus_tag="AC159P_22045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3698181.1"
                     /translation="MSYLDVPRLHFSGKFIAQPSTLNNTPSNFDPATTQPVPLWNPNG
                     NHTWQLVNCGVQSVVFADGKSVYAPQQINATVSSTDKPVTAKLVDLDTQQQLVSQIWG
                     LRIKVAFSKTDYFVGDFRVVCFDDITFARSKTGGGSSRASAFFQSFLDNVEWGGYLTS
                     PFLRQLQSVSPARLSVKFVVDGYDMNTKQGRVVGTIGPAWADEPPNFVLGRALRVNRQ
                     FQPEDSINYGYARVDPIRKRLLVDLGNSIPTVRPGGPPPETLGTLEVVIGPSTSSPIA
                     LGKYDYSEAAYLHFAGIQEFPLDAKQLAALTNTPLGVRQTNATGAVTVPLQEGANGTY
                     VCATQQVYRMNPSDTASVELIALRFGQPAGGETIDLAQVTGSTQTIGPPPPPVGTPAA
                     ALTLPSSVTTGDDGRASFCLTASDPSNPRGFIDGQVYAVGYNWAADDDPAF"
BASE COUNT          980 a         2376 c         2014 g          993 t
ORIGIN      
        1 ccgctaccac agccgaccga ccgaggttcc ggagagcggc gggtcaagct cggcctcggc
       61 acggacgcat atggctcgtc tcctcatccg cgctgcaatc gccaccgtct ggctcgcgct
      121 ctgccacgtc gcgctcgcgg cgcaggcggt tcccgcgggg gccacgctcg atggcaccgt
      181 cacggatcag cacggcgcgg tcgtcgtcgg cgcagaggtg cgcgccctga acatcgcgag
      241 cgggcgcacg ctcgcggcgc ggacggacgc ggacgggcgg tacgagctga cgggcctcgc
      301 gcccggcccg taccggttga gcgtgagcgc ggcgggcttc acggcggcgg cgcggcgcgt
      361 ccgcgtcgag cgcgcgcccg accccgtcag cgaagacttc gtgctggtgc ccggcatcgt
      421 cgagagcagc gtcacggtga cggcgggcaa gggcagcgcg cgcgccgtcg ccgagacgcc
      481 gcagagcgtc accgtcagcg acgccgcgcg catcgaaggg cggcggcccg cctcgacgct
      541 tcaggccgtc gagcaggcgc cgaacctcac gcccgtcatc gccaacccgg cgctcgaacg
      601 cccgcgcctg cgcggactgg cctcgaaccg cctcctcgtc atcctcgacg gcgagcgtct
      661 caacaacatc cgcagcgacc cgacgagcgg cgtctcgccg agcgtcgtcg atgtcacgca
      721 gctcgaatcg gcggaagtgc tcggcggcgc aggctccagc ctctacggct cggacgccat
      781 gtccggcgtc atcaacctca tcacgcgcgg ccccgtcagc gccggagacg gcgaccccgt
      841 tttgggtttc cgtttcgacg gcgacgcgcg caccaacggt cgcttccgcc gcggcgcggg
      901 cgtcgtcaac gcgagcgtcc cgcgcctcgc cttccgcttc ggcggctcgc tcttccgcgc
      961 gggcgactac gacgcgggcg accgcgccat cccgctcgaa gacgttgtca gactcggcaa
     1021 tctggcgacc gagatgagca acgccggcgg cggcggcgtc gcgcgcacgt acgccgtgtg
     1081 gagcctgccc gcgggcgcgc gcgttccgaa cgggcaggcg cgcggcttca acgatcaggt
     1141 ggattcgtgg ttcttcccac gcgccaatca cggtcttcgt tatcgtcaac tgaacagcca
     1201 gcataagaac ctcggcttct ccttcatcgc gccgcccttc gaccagcgga cgcagttcaa
     1261 cgccttccgc cgtctcgaca agtacggcct gcgctacgag gcgcgcgagc tgctgccgtg
     1321 gctgccgcgg ctcgccgtcg gcttctaccg gcagaagtac tccttccccg acgacaccat
     1381 cacttcgccc atcgacgccg acagcagttg gactctcgtc cccaacccgg acgacccgca
     1441 gacccccttc gcagtcctca cgggccgccc ctcgaccttc accgacgccg gcttcaccga
     1501 caacaagaac tcgatcacca cctgggcctt cgacgcgcag gccaccttcg cgcccttcac
     1561 gggcacgctc ttcacgaccg gcgtcggcta cctgcgcgac tcttcggcgg acgagttctc
     1621 gcgcgtcgag ttcgtccccg gcacgcgcga cgtgcgcact tccatcagcg gtcgcgccag
     1681 caaccccgac tcggtctacc gcaactgggg ctggttcaac ctgctggaat acgagccggt
     1741 gcgctggctg cgtctcgtgg gcggcctgcg cgtcgataac tggcggactg aagcgcgcgc
     1801 gacgcgcggc ttcccgctcg gcaacgaggc ggccatactc gacgcctcgt tcgcgcaact
     1861 gctcgccaac cccggccaga taaatacaga cggcgccgca ggcatcgccg acctgctcga
     1921 cggcgacggc tccatcaaca cctcgcgcac ggtcacgacc ggcaacgtcg gcgtcgtctt
     1981 tcgtctgccc cacggcgtca acccgtacct gcgctggggc aacagctacc gcgagccggg
     2041 catcaccgag cggtatctgc tgcgcgactt cggcgacccg gccttcagcg tgctcgtcgt
     2101 cccgaacgcc tcgctcaagc cggagcgcgg cgagagcttc gacctcggcg tgaaggttca
     2161 acgcgcgcgg ctgttcgcct ccgccggtta ctttcgcaac cgcctcgaag acttcatcgg
     2221 caacgccttc gccgcacccg tcttcgtgcc cgccgacccg gcgcgcgggc tgcaacccct
     2281 gtcgcccttc ttccccttcc acggcgtcct ctacgtgcag cgcacgaaca cggcgcgcgc
     2341 acgcatcgag ggcttcgagg ccgtctacga gctgagcctg ccgctcggcg agcgcggcgt
     2401 cctgacgccg ttcggcgcgc tcggctggct caagggtgcg aacctcacgc ccgacgaaac
     2461 gacgctcgcg ctcatcgaac gattctataa ccgcgacgac acgcccatcc ccttgcgcgg
     2521 ctccgccgag gacgcgccgc tgacgggcat cacgcctttc cgcatggtcg gcggcgtgcg
     2581 ctacgacagc ttccgccgcc gctggttcgg cgagttcgag ctgcgctatc aggggcgcgt
     2641 gcggcgcgtc gatccgctcg atctgacgac ggccatctcg acgcagtacg gctcgctcgc
     2701 cggactcgac tctttcgccc gccacacgtt gcgcgcgggc tacacgctgc ggcgcgagag
     2761 ctaccgcgcc gcgttcacgc tcggcgtgga gaacctgacc gaccgcctct acttcgagca
     2821 ctttcagacg gcgcccgcgc cgggccgctc ggtcgtcttc gggctgacgc tcgacctctt
     2881 cgacctgctg cggaagtaga ccgcgtcgag attcgtggcc ggactttttg tgcgacgaga
     2941 agtaaaggga tgaaacgtta cctgctggcg gcgtgcgccg tcgtcttcgt catgacgatt
     3001 ctgttcgcgg cggtcgaggc tctgggcgtg ccgctgctga cgaaccccgc gccgtggatg
     3061 aagggcggcg gggcgttggc cgcgtgcgtg ggcgtggggc tgctcgtcgc ggacgtgctg
     3121 ctgcccgtgc cgtcgagtct ggtgatggtg gcgcacggcg ctctgttcgg cgtggcgggc
     3181 gggacgctgc tctcgctcgc ggggagcctg ggcgcggcgc tcgtcggatg ttggatcggg
     3241 cggcgcggcg ggcgtctgct ggagcgcgcg gtgaagacgg tgaagccgga cgaggcggcg
     3301 cgcgccgact atctgctgcg gcgttggggc gcgctggcga tcatcgtgac gcggcccgtg
     3361 ccgctgctgg ccgagacggt ggcgctgctg gcgggcgctt cgcggatgag ctgggggcgc
     3421 gtcgcggcgg cggcgctctt aggctcgctc ccgcccgcgc tgctctacgc gctgacgggc
     3481 gcggcggtcg ccaaccttca gagcacggcg ttgatgttcg tcctcgtcat tctggtcgcg
     3541 ggtttcttct ggatgctcgg tcggctgctc gaccccttcc gccgccgcgg agaagggaca
     3601 cgacgcttcc gcgacgcttc ctcgttcccc tccctcgaac cgcaacgaaa ggagaactct
     3661 catggcgatt aaactcacca tcaacggcag ccccggcccc gaaggcttcc tcgtcgcccc
     3721 ggcgggcaga caaacttttc cggccatcgt cgggctgaag accgacgatg gcacgaccgt
     3781 gcaggcgaag cttcaggtag tggcgccgaa gggcgtggtc gtcgtcgtga gtcccaccac
     3841 gctctccatc ggccccacgc agaagaccgt caagcttacg gccaagacgc cgagcaagag
     3901 tcagggcgac atctcgtttc aggtgaaggt cggcctgacg gtcaaggcca agctcaagct
     3961 cacctgcgtc accgatccgc gcctccgctt cggcggtcgt ttccaggcgc gcttcgccac
     4021 cgacggcgac ttcttcaaca acgcgcgcgg cacgagcgcc gggtggacgt gggccctgga
     4081 gggcgagccg gacttcgtgc ccgcggcgaa caacgtcccc acggcgccgg gcatggccgt
     4141 cgggcgcgtg gtgcgcttcc agaacgccgt ggcgccgcgc cccctcgtcg cgcccatcgg
     4201 cgtcacggtc acggcggtcg aggccacgct cggcacgaag acggtctcct tcacgaaagg
     4261 cgacgccgtc atcggccaga aggtcgaact cggccccaac tcgtacctcg cctcgaaccg
     4321 accggcgaac cctgcgctcc cgccgccctt cgagaactac ggccccggct tcgagccgat
     4381 agagaacttc gtgttcgaag tcggcgcgct cttcagcggc aagcccgcct cgctcaacga
     4441 ccgcccgaag gccaacggct tcttcccgct cacgccgacg cagctcacgc agtacggcat
     4501 cgtcgcgctg agcaccttca acacgcagcg caagaacgcg ctgcttgccg cctacaacgc
     4561 gctctcgccc gcagaccgca cgggcacgcc cgccgggcgc aacctcgcca cgcgcatctc
     4621 gcacctcggc ggcttgcccg cccacagcat cccgcccacg caggcgacgc tctcgttggg
     4681 gtggagcgga caggagacct acaacggcct cgtcaacagc gcgatcatga tcaccgcgac
     4741 caagtcgccc gtgctcgcct acctcaagac gttccagtcg ttccgctact ccggcatgtt
     4801 cttcaacttc cactccgacg aactctgcgg tcgggtggac ggcacgctcc gcgcgatgac
     4861 gctcgcgctc tcgcgcctgc cgccgcagga ataacgcacg ggattgcgga atgcggattg
     4921 cggattgatg gccgtcgagc tggaagcttc gaaattcaaa gttgagcgaa gactcagcct
     4981 taatccgcaa tccgcattcc gcaatccaca acgacctgac gaaggagtag accatgagct
     5041 atctcgacgt tccgcgcctt cacttttccg gcaagttcat cgcccagccc tcgaccctca
     5101 acaacacccc gtccaacttc gacccggcca ccacacagcc cgtcccgctc tggaatccca
     5161 acggcaacca cacctggcag ctcgttaact gcggcgtgca gtcggtggtg ttcgccgacg
     5221 gcaaaagcgt gtacgccccg cagcagatca acgccacggt cagcagcacg gacaagcccg
     5281 tcacggccaa gctcgttgac ctcgacacgc agcagcagct cgtctcccaa atctgggggc
     5341 tgcgcatcaa ggtcgccttc tccaagacgg actacttcgt cggcgatttc cgggtggtct
     5401 gcttcgacga catcaccttc gcgcggtcga agacaggcgg cggctcctcg cgcgccagcg
     5461 ccttcttcca atccttcctc gacaacgtgg agtggggcgg ctatctcacc tcgccgttcc
     5521 tgcggcagct ccagagcgtc agcccggccc gcctctccgt caagttcgtc gtcgatgggt
     5581 acgacatgaa caccaagcag ggacgggtcg tcggcaccat cggcccggcg tgggccgacg
     5641 agccgccgaa cttcgtcctc gggcgcgcgc tccgcgtgaa ccggcagttt cagccggaag
     5701 actcgatcaa ctacgggtac gcgcgcgttg acccgatccg gaagaggctg ctcgtagacc
     5761 tcggcaacag catccccacg gtcaggcccg gcggcccgcc gcccgagacc ctcggcacgt
     5821 tggaggtcgt catcggccct tcgacaagca gccccatcgc cctcggcaag tacgactact
     5881 cggaggccgc ctacctccac ttcgcgggga ttcaggagtt ccccctcgac gccaagcaac
     5941 tggccgcgct caccaacacg ccgctcggcg tgaggcagac taacgcgacg ggggccgtca
     6001 ccgtcccgct gcaagaggga gcgaacggga cgtacgtctg cgccacgcag caggtctacc
     6061 ggatgaaccc gagcgacacg gcgtcggtcg agctgatcgc cctccggttc ggccagcccg
     6121 ccggcggcga gaccatcgat ctggcgcagg tcaccggcag cacccagacg attgggccgc
     6181 ccccgccgcc cgtcggcacg cccgccgcgg ccctgacttt accgtcgagc gtcacgacgg
     6241 gcgacgacgg tcgcgcctcc ttctgcctga cggcctccga ccccagcaac ccgcgcggct
     6301 tcatcgacgg gcaggtctac gccgtcggct acaactgggc cgcggacgac gaccccgcgt
     6361 tcc
//
LOCUS       DBNGOH010001133         4191 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2557571, whole genome shotgun sequence.
ACCESSION   DBNGOH010001133 DBNGOH010000000
VERSION     DBNGOH010001133.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4191)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4191)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4191
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2557571"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..988)
                     /locus_tag="AC159P_22050"
     CDS             complement(<1..988)
                     /locus_tag="AC159P_22050"
                     /inference="COORDINATES: protein motif:HMM:NF025684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4384 domain-containing protein"
                     /protein_id="HIR3698182.1"
                     /translation="MRFKLFAGLLALALLAPFVAPPFAQDSTPQQDDQDIIDDFVTTR
                     GASFGERPKKPANTNTSGNTQPKKAFKSPAGEKAVKSPAGETAAGNKKSGGLGVFKKK
                     TPPRETVATNTANKNTAGLGGTNAVANTNAATNVNTNAGKQADEVPAVEASAEAPGGP
                     IGLGYTILSVAGDGSNTVVDSARAFAAGDKIRIALETNADGFLYIFNAENDGPAPLML
                     YPHAALDGGANRIAAHVRDFFPADLRYSFEFDDAAATEHLYVIFSRRPLADVPAGAAL
                     LAHCGGAARDDCYWKPAPEQWGRLKAAASAGRVAEKRNDDLAKAQTPPPVPSL"
     gene            complement(1051..3141)
                     /locus_tag="AC159P_22055"
     CDS             complement(1051..3141)
                     /locus_tag="AC159P_22055"
                     /inference="COORDINATES: protein motif:HMM:NF019334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR3698183.1"
                     /translation="MKLCPTCNNSFEDTLSFCPRDGEVLQDLPENLVGHVLDEKYELE
                     SFIAQGGMGAVYRARHILLGDRVVIKMLRPEMRQNAEWLRRFQREGRAARSFRHPNAV
                     TVYDLSSAAGGHIYMVMEYVEGHTLDKELKRRGQLTPHEALEVLEPVADALDAAHARG
                     VVHRDMKPENVMLGAGDHGEMVVKLLDLGIAKMVGVADAMAGEATSLTVAGQILGTPY
                     YMSPEQWGEPARDGHAEIDGRTDIYSLGVIFYELVAGRKPHNGRTLSEMRLAHVRGTP
                     PDLGQLAPGVPEGFVRAVERSMAKDRADRPQTAGELIDELRASLGLPARARTRSARGD
                     VHATAEPARDGAATNQGAHGNEAATANFKPPVSAGELGRAKTILTSDFETPADDADGR
                     KTAAQNARPATSANVAARGDGARETSAHHSHETEAAVAADKRLGETRAAEHIAEDQAA
                     GHLAGRPKRSYAPLVAGGVLVLLLLAVAGGRLAWRRFIAPPTTDDPTKTAGAENTPEA
                     PPAAPTKVEAVSYWIESFDVPKEGDVQRVAEVSAPLLSGQHFKFHFTTRERGYLYIIG
                     PGEQGNAQMTFFTAQGGGLLKTNLVAADSDFAFPSGEDKTLVLDEHSGIEEYTVIFSP
                     TPLLKPAFLAEKNLKPLSPAEVKELEDFRAEHKTAAASLAVNDGGGGRRVAVSVPEGA
                     GGRPVIFDIRIDHK"
     gene            3532..>4191
                     /locus_tag="AC159P_22060"
     CDS             3532..>4191
                     /locus_tag="AC159P_22060"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HIR3698184.1"
                     /translation="MTSVSRVRFFSLLVIVLLLPVASSAQEDEEVVKVETNLVSMPVV
                     VTTRGGEYVADMRQEEFSVFEDGAAQQVAFFATITQPFHVVFLLDTSGSTEEKIGFIR
                     RSAIAFVAELQRADRVRVISFDDEVRVLNAEFTGDQAEVRRAIDSIRPGKGTRLYDAM
                     QLAFNLLRPVKGRKAVVIFTDGVDYRSHEYSYANNIRALEESDVIVYPIRYETRAETE
                     RL"
BASE COUNT          577 a         1413 c         1462 g          739 t
ORIGIN      
        1 cgagcgaggg cacggggggc ggggtctgcg ccttggcgag gtcgtcgttg cgtttctccg
       61 cgacgcggcc cgcgctcgcc gccgctttca accgccccca ctgttcgggc gcgggcttcc
      121 agtaacagtc gtcgcgcgcg gcgccgccgc agtgcgcgag cagcgccgcg cccgcgggca
      181 cgtccgcgag cgggcggcgc gagaagatga cgtagaggtg ttcggtggcc gccgcgtcgt
      241 cgaactcgaa agagtagcgc aggtcggcgg ggaagaagtc gcgcacgtgg gcggcgatgc
      301 ggttggcgcc gccgtcgagc gccgcgtgcg ggtagagcat cagcggcgcg gggccgtcgt
      361 tctcggcgtt gaagatgtag aggaagccgt cggcgttcgt ttcgagcgcg atgcgtatct
      421 tgtcaccggc ggcgaaggcg cgcgccgaat cgacgaccgt gttcgagccg tcgcccgcca
      481 ccgacaggat cgtgtagccc agcccgatgg ggccgcccgg cgcctccgcc gacgcctcga
      541 ccgcgggcac ttcgtccgcc tgcttgcccg cgttcgtgtt cacgttcgtg gccgcgttcg
      601 tgttcgcgac cgcgttcgtg ccgcccagcc cggcggtgtt cttgttcgcc gtgttcgtcg
      661 cgaccgtctc gcgcggcggc gtcttcttct tgaagacgcc gagtcccccg cttttcttat
      721 tgccggcggc ggtctcgccc gcaggcgact tgaccgcctt ctcgcccgcg ggcgacttga
      781 acgccttctt cggctgcgtg ttgccggacg tgttcgtgtt cgcgggcttc ttcggtcgct
      841 cgccgaatga cgcgccgcgc gtggtgacga agtcgtcgat gatgtcctgg tcgtcctgct
      901 gcggcgtgga gtcctgcgcg aagggcggcg cgacgaaggg ggccagcagg gccagcgcga
      961 gcagtcccgc gaaaagcttg aagcgcatgt ctcgactcct cactgtcttc ccaaagaacg
     1021 cggacgcgcg ggagagtacg gcgcggcgtc tcacttgtgg tcgatacgaa tatcgaagat
     1081 gacgggccgc ccgcccgcgc cttcgggcac ggagacggcg acgcgccgac cgcctccgcc
     1141 gtcatttacg gccagcgaag cggcggcggt tttgtgctcg gcgcggaagt cctcaagctc
     1201 tttgacctcg gcgggcgaga gcggcttcag gttcttctcg gcaaggaagg cgggcttcag
     1261 cagcggcgtc ggcgagaaga tgactgtgta ctcttcgatg ccggagtgtt cgtcgaggac
     1321 gagcgtcttg tcttcgccgg aggggaaggc gaagtccgag tcggcggcga cgaggttcgt
     1381 cttcaacagc cccccgccct gcgcggtgaa gaaggtcatc tgcgcgttcc cctgctcgcc
     1441 cggcccgatg atgtagagat agccgcgctc gcgcgtcgtg aagtggaact tgaaatgctg
     1501 gcccgagagg agcggcgcgg acacctcggc gacgcgctgc acgtcgccct ccttcggcac
     1561 gtcgaaagac tcgatccagt agctcacggc ctcgaccttc gtcggggcgg cgggcggcgc
     1621 ttccggtgtg ttctctgcgc ccgcggtttt cgtcggatcg tccgtcgtcg gcggcgcgat
     1681 gaagcgtcgc cacgcgagcc ggccgcccgc gaccgcgagc aacaacagca cgagtacgcc
     1741 gcccgcgacg agcggggcgt acgagcgttt gggccgcccc gcgaggtgcc ccgccgcctg
     1801 atcttcggcg atgtgctccg ccgcacgtgt ctccccgagg cgtttgtccg cggcgacggc
     1861 ggcctccgtc tcatggctgt ggtgcgcgga agtttcgcgc gcgccgtcgc cgcgcgcggc
     1921 gacgttggcg ctggtggcgg gccgcgcgtt ctgcgcggcg gtcttgcgcc cgtccgcgtc
     1981 gtcggcaggc gtctcgaaat cggaggtgag aatcgtcttg gcgcggccca gctcgcccgc
     2041 ggagacgggc ggcttgaagt tcgccgtcgc cgcctcgttg ccgtgtgcgc cctggttggt
     2101 cgcggcgccg tcgcgcgccg gttccgccgt cgcgtgaacg tcgccccgcg ccgagcgcgt
     2161 ccgcgcgcgc gcgggcagac cgagcgacgc gcgcaactcg tcgatcaact cgccggcggt
     2221 ctgcggtcgg tcggcgcgat ccttcgccat cgaccgctcg accgcgcgca cgaagccctc
     2281 gggcacaccg ggcgcgagct ggccgaggtc gggcggcgtg ccgcgcacgt gcgccagccg
     2341 catctccgag agcgtgcgcc cgttgtgcgg cttgcggccc gcgaccagct cgtaaaagat
     2401 gacgccgagc gagtagatgt ccgtgcgccc gtcgatctcg gcgtggccgt cgcgcgcagg
     2461 ctcgccccac tgctcgggcg acatgtagta gggcgtgccg aggatttgac cggcgaccgt
     2521 gagcgaggtg gcctcgcccg ccatcgcgtc cgccacgccg accatcttgg cgatgccgag
     2581 gtcgagcagc ttgacgacca tctcgccgtg gtcgcccgcg ccgagcatca cgttctcggg
     2641 cttcatgtcg cggtggacga cgccgcgcgc gtgcgccgcg tcgagcgcgt cggccacggg
     2701 ttcgagcact tcgagcgctt cgtgcggcgt gagctggccg cgccgcttca gctctttgtc
     2761 gagcgtgtgc ccctcgacgt actccatcac catgtagatg tgcccgcccg cggcgctgct
     2821 gaggtcgtag acggtgacgg cgttcgggtg gcggaaactg cgggccgcgc gcccctcgcg
     2881 ctggaagcgg cgcagccatt cggcgttctg ccgcatctcg gggcgcagca tcttgatgac
     2941 cacgcggtcg cccaagagga tgtggcgcgc gcggtagacg gcgcccatcc cgccctgcgc
     3001 gatgaagctc tccagctcgt acttctcgtc gagcacgtgc ccgacgaggt tctcgggcaa
     3061 gtcctgcaac acctcgccgt cgcgcgggca gaagctcagc gtgtcctcga agctgttgtt
     3121 acatgtcggg cataacttca tcgcgggctg tccaacggcg tgtcgctgct ccggtgccgt
     3181 gcgggttaaa agtctaacac gccgcatcat gcgggcgaag cgtaagcccg atgaagaagc
     3241 aggcagcagg cagcaggcag gaggcagaca gaacttttcg gatttgtgga ttttggattg
     3301 aagagatggt tgacgggggc ggagctgtca tcaatcgtcg gtcgtcatcg tcaaccgtca
     3361 atccaaagtt cgaagtccga atttttcttg tctgcctact gcccgctgcc tgctgcctac
     3421 tatttcctct tgccgttttc tcggccccgg cgtttaatag ggcgcacacg tctcccttcc
     3481 caacgtgtcc gtcggccagc cgtaccttcg aggcgcgaga gggcgccgcc catgacaagt
     3541 gtgtcgagag tccgcttctt ctccctgctc gttatcgttc tgctccttcc cgtcgcgtcg
     3601 tcggcgcagg aggacgagga ggtcgtcaag gtcgagacga acctcgtctc gatgccggtc
     3661 gtcgtgacga cgcgcggcgg cgagtacgtc gccgacatgc ggcaggagga gttcagcgtg
     3721 ttcgaggacg gggccgcgca gcaggtcgcc ttcttcgcca ccatcacgca gcccttccac
     3781 gtcgtcttcc tgctcgacac cagcggcagc accgaggaga agatcggctt catccgccgg
     3841 tcggccatcg ccttcgtcgc ggagctacag cgcgccgacc gcgtccgcgt catctcgttc
     3901 gacgacgagg tgcgcgtgct gaacgcggag ttcacgggcg accaggcaga ggtgcggcgg
     3961 gccatcgact cgatcaggcc gggcaagggc acgcggctct acgacgcgat gcagctcgcc
     4021 ttcaacctgc tcaggccggt gaaggggcgc aaggccgtcg tcatcttcac ggacggcgtg
     4081 gactaccgca gccacgagta cagctacgcc aacaacatcc gcgccctcga agagtcggac
     4141 gtgatcgtct accccatccg ctacgagacg cgcgccgaga ccgagcggct c
//
LOCUS       DBNGOH010001134         3442 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3290039, whole genome shotgun sequence.
ACCESSION   DBNGOH010001134 DBNGOH010000000
VERSION     DBNGOH010001134.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3442)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3442)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3442
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3290039"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            173..1774
                     /gene="serA"
                     /locus_tag="AC159P_22065"
     CDS             173..1774
                     /gene="serA"
                     /locus_tag="AC159P_22065"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HIR3698185.1"
                     /translation="MSDIKIFIADDVNESGLAPLRAAGFAVEKRTGLKGDELFEALRD
                     ADGLLVRSETKVTAAAMDAAGRLRAVGRAGVGVDNIDVAAATARGIVVMNAPDGNTMT
                     TAEHTLALLLALARRVPQGDASMKGGRWERKRFVGVELRGKTLGIVGLGRIGRVVASR
                     ARGFEMKVVAFDPFITPEQARDAEIELAPLEEVCTRADFLTVHTPLTQETRGLISARE
                     FALMKEGVRVINCARGGLVDERALHAAITEGKVAGAALDVFEEEPPPPGHPLLSLEEV
                     IVTPHLGASTREAQEGVALTVAEQMRDFFLTGALRGAVNVPAVGAQELTALQPYIELA
                     ARLGRFVAQLVDDPVREVAIEYAGEVAGMDAAPVTRAFLAGLLKNMSARVNAVNALLI
                     AGERGIVVTDTYKRGGGPGDMTPAVRTRVVTTTGERTASGALFGQRSDGRITELDGFR
                     VEVIPAGNMLVTRSSDVPGVIGRVGTALGEGGVNISRFHLGRRERGGEAIAVIETDAP
                     LDDATLARLRSFREIISARRIELEP"
     gene            complement(1782..2084)
                     /locus_tag="AC159P_22070"
     CDS             complement(1782..2084)
                     /locus_tag="AC159P_22070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3698186.1"
                     /translation="MLEVIVLIFLTRRVGEIVEEKGRRSGWYKALAVVLWIGGEAIGA
                     LVGLAVSGHFDSGKVLMRIFCVLLGVAAGAALAFVIAKSLSPVAAYNTPPPPPTFG"
     gene            complement(2244..>3442)
                     /locus_tag="AC159P_22075"
     CDS             complement(2244..>3442)
                     /locus_tag="AC159P_22075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3698187.1"
                     /translation="RAFDPSGRLAATEFAELRNSQEPLFTLAVDTGGRALVNTNALDA
                     AVEKALAETSVYYLLAWRPEGVAANAPRFRRIEASVKGKSDLRVVVRKGFFDTPPPEP
                     TAVVKKDSKKQPPPQPIPPPPAADKQLFDALKSRHPRVSLPTSLALGYLSTPAHGVVM
                     TASLGIDRELLAPPAPEAKEGARVDVAGAVYNDKGDAVSVFKQELAVHPSATPGEGRH
                     SILYSHSFRLAPGLYQVRVAALERRSGRAGSASQWVEVPALGQGQFSMSSLFIGERPA
                     SAAANTTPAADTESGVLLSVDGRFARNSAMRFVTYVYNATAGANSAPPDVALQVQMFR
                     DDQPVFTAPLLKVKTAGVADLTRIPYAAELSLASFPPGRYLLQITALDRVARTSTTRR
                     VKFIID"
BASE COUNT          519 a         1096 c         1332 g          495 t
ORIGIN      
        1 aggcggcggc ggtcgtgccg acggcagcga cacgggcggg ccgatgtgaa caacaagtag
       61 gcagtaggcg gtaggcagta ggcagccaaa acagttggag aatataaatc ttcaactgtg
      121 aaagctgacg agtctttact gtctactgtc tactgtctac tgcctactaa ctatgtccga
      181 cataaaaata ttcatcgccg acgacgtgaa cgagagcggg ctggcgccgc tgcgtgcggc
      241 gggcttcgcg gtcgagaaga ggacggggct gaagggcgac gagctgttcg aggcgctgcg
      301 cgacgcggac ggcctgctgg tgcggagcga gacgaaggtg acggcggcgg cgatggacgc
      361 ggcggggcgt ctgcgcgcgg tggggcgtgc gggcgtcggc gtggacaaca tcgacgtggc
      421 ggcggcgacc gcgcgcggca tcgtcgtcat gaacgcgccg gacggcaaca cgatgacgac
      481 cgccgagcac acgctggcgc tgctcttggc gctcgcgcgg cgcgtgccgc agggcgacgc
      541 gagcatgaag ggcgggcgct gggagcgcaa gcggttcgtc ggcgtggagc tgcgcgggaa
      601 gacgctgggc atcgtcggac tggggcgcat cggtcgcgtc gtggcgagcc gcgcgcgcgg
      661 cttcgagatg aaggtcgtcg ccttcgaccc gttcatcacg ccggagcagg cgcgcgacgc
      721 ggagatcgag ctggcgccgc tcgaagaggt ctgcacgcgc gcagacttcc tcaccgttca
      781 cacgccgctc acgcaggaga cgcgcgggct gatctcggcg cgcgagttcg cgctgatgaa
      841 ggagggcgtg cgcgtcatca actgcgcgcg gggcgggctg gtggacgagc gcgcgctcca
      901 cgcggcgatc acggaaggta aagtggcggg cgcggcgctc gacgtgttcg aggaggagcc
      961 gccgccgccc ggtcacccgc tgctctcgct cgaagaggtc atcgtcacgc cgcacctcgg
     1021 cgcttcgacg cgcgaggcgc aggagggcgt cgcgttgacg gtcgccgagc agatgcgcga
     1081 cttcttcctg acgggcgcgc tgcgcggcgc ggtgaacgtc cctgcggtcg gcgcgcagga
     1141 gttgacggcg ctccagcctt acatcgagct ggccgcgcgg ctcggcaggt tcgtggcgca
     1201 gttggttgac gaccccgtgc gcgaggtcgc catcgaatac gcgggcgagg tcgcggggat
     1261 ggacgcagcg ccggtgacgc gcgcgtttct ggcgggcctg ctgaagaaca tgagcgcacg
     1321 cgtgaacgcc gtcaacgcgc tgctcatcgc gggcgagcgc ggcatcgtcg tgaccgacac
     1381 ctacaagcgc ggcggcggcc ccggcgacat gacgcccgcg gtgcggacgc gcgtcgtgac
     1441 gacgaccggc gagcgcacgg cgtcgggcgc gctcttcggg cagcgctcgg acgggcgcat
     1501 cacggagctg gacggcttcc gcgtcgaggt catcccggcg ggcaacatgc tggtgacgcg
     1561 gagcagcgac gtgcccggcg tcatcggtcg cgtcggcacg gccctgggcg agggcggcgt
     1621 caacatcagc cgcttccatt tgggccggcg cgagcgcggc ggcgaggcca tcgccgtcat
     1681 cgaaacggac gcgccgctcg acgacgcgac gctcgcgcgc ctgcgctcct tccgcgagat
     1741 catctcggcg cggcgcatcg agcttgagcc gtgagagttt gtcagccgaa cgtgggcggc
     1801 gggggcggcg tgttgtaggc ggcgaccggc gagaggctct tggcgatgac gaacgcgagc
     1861 gccgcgccgg cggccacgcc gagcagaacg cagaagatgc gcatcagcac cttgccgctg
     1921 tcgaagtggc ccgacacggc gaggccgacc aaggcgccga tggcctcgcc gccgatccag
     1981 agcacgacgg ccagcgcctt ataccacccg ctcctgcggc ccttctcctc gacgatctcg
     2041 cccacccgcc gggtcaggaa gataagcacg ataacctcta gcatggcgct ctcctgtcgc
     2101 gtctcggcgt gacggggcgc ggcgggtcgg gccgcgcgcg ggccgccggt gggaaactga
     2161 cggccccgcc gaaagacggg aagcaagata tcgtaggtcg gcgccgggtg tgaagtcggg
     2221 acgggcggag ccgaacgtct gcgctagtcg atgatgaact tgacgcgccg cgtggtgctc
     2281 gtcctggcga cgcggtcgag ggcggtgatt tgcagcaggt agcgtcccgg cgggaaggac
     2341 gcgagcgaga gttccgccgc gtaggggatg cgcgtgaggt ctgcgacacc agcggtcttg
     2401 accttgagga gcggggcggt gaagacgggc tggtcgtcgc ggaacatctg tacctgaagc
     2461 gccacatcgg gcggcgcgga gttcgcgccc gcggtcgcgt tgtagacgta ggtcacgaac
     2521 cgcatcgcgg agtttcgcgc gaagcgccca tccacgctca gcagcacgcc cgattccgtg
     2581 tccgcggcag gtgtcgtgtt cgccgcggcg ctcgccggtc gctcgccgat gaagaggctg
     2641 ctcatcgaga actgcccctg gccgagcgcg ggcacctcga cccactgcga ggcgctgccc
     2701 gcgcgcccgc tccggcgttc gagcgcggcg acgcgcacct gatagaggcc gggcgcgagc
     2761 cggaacgagt ggctgtagag gatgctgtgg cggccctcgc ccggcgtcgc ggaggggtgc
     2821 acggcgagct cctgtttgaa gacggagacg gcatcgccct tgtcgttgta gacggcgccc
     2881 gccacgtcca cgcgcgcccc ctccttcgct tcgggcgcgg gcggcgcgag tagttcgcgg
     2941 tcgatgccga gcgaggccgt catcacgacg ccgtgcgcgg gcgtgctgag gtagccgagc
     3001 gcgagcgagg tcggcaggga gacgcgcggg tggcgcgact tgagcgcgtc gaagagttgt
     3061 ttgtccgccg cgggcggggg cgggatcggc tgcggcggcg gctgcttctt cgagtccttc
     3121 ttcacgaccg cggtcggctc gggcggcggc gtgtcgaaga aacctttgcg cacgacgacg
     3181 cgcaaatcgg acttgccttt gacgctcgcc tcgatgcggc ggaaccgggg cgcgttcgcg
     3241 gcgacgcctt cgggcctcca cgcgaggagg tagtagacgg aggtctcggc gagcgccttc
     3301 tcgaccgccg cgtcgagcgc gttggtgttg acgagcgcgc gcccgcccgt gtccacggcg
     3361 agcgtgaaga gcggctcctg cgagttgcgc agttcggcga attccgtcgc cgcgagccgc
     3421 cccgaggggt cgaaggcgcg gt
//
LOCUS       DBNGOH010001135         3238 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4938951, whole genome shotgun sequence.
ACCESSION   DBNGOH010001135 DBNGOH010000000
VERSION     DBNGOH010001135.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3238)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3238)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3238
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4938951"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            91..237
                     /locus_tag="AC159P_22080"
                     /pseudo
     CDS             91..237
                     /locus_tag="AC159P_22080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013928254.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6766 family protein"
     gene            392..1120
                     /locus_tag="AC159P_22085"
     CDS             392..1120
                     /locus_tag="AC159P_22085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003806352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HIR3698188.1"
                     /translation="MKVHVGTSGWSYAHWHGVLYPHGAHPRERLDYYLLRYRTAELNS
                     SYYRWPADASFSRWRRRLPEDFTLCVKAPGLLTHVSRLYGPERWLARVAGSLSRLGGK
                     LGVLLVQLSPRFAYDHARLAYFLDRVPAGLRVAVEFRHPSWHREEVFGLLGRHGAGYC
                     VMSGARLPCVLRATAPFVYVRLHGPDHEHLYAGSYPEQDLRWWACRIREWVSTGREVF
                     VYFNNDGGGNAVRNAAALKAILGL"
     gene            complement(1403..1645)
                     /locus_tag="AC159P_22090"
     CDS             complement(1403..1645)
                     /locus_tag="AC159P_22090"
                     /inference="COORDINATES: protein motif:HMM:NF012920.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HIR3698189.1"
                     /translation="MSESAANILQFRPSNRPETFTLRLSDDSMIGAGLPAGSLLTFTT
                     SADYLPGECVAVETPDGLFVKYMAIIYLTHLLGNCG"
     gene            complement(1922..2329)
                     /locus_tag="AC159P_22095"
     CDS             complement(1922..2329)
                     /locus_tag="AC159P_22095"
                     /inference="COORDINATES: protein motif:HMM:NF013098.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR3698190.1"
                     /translation="MTGLQFATAVIYTEDVAAAVAFYERATGLTASYYDRELGFALLG
                     AEQTVAVASHVAGTLMLEAGYAPVLSGRVRGTELAFWAEDVPAAFEAAVRAGATALTP
                     PRVMPWGQTVAYVEAPEGTILGFVTRVPSPDPG"
     gene            complement(2431..2991)
                     /locus_tag="AC159P_22100"
     CDS             complement(2431..2991)
                     /locus_tag="AC159P_22100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HIR3698191.1"
                     /translation="MFCPKCGRDDSPRHKFCPACGTNLDVVTIALTTGEDSIFTRVNK
                     HLDRSVAHYADHFFESATTTGREGQVGSSWRLVGKGMLTFFLDLALLPVMFFFLPIRL
                     LMLLLYTPLGLLQKRSERKRSGVPSLDGASESSKRGLPEAGGWRGGSVGSVTENTTVH
                     LELAEASEGRTKTSDDIGGKARITTR"
BASE COUNT          574 a         1085 c         1015 g          564 t
ORIGIN      
        1 cacttgaatc gtaaacgccg ccgaacgggg gcgcgctttg agtagcggcg cccccgttcc
       61 cctcatgcca cagcaggaca agcgacctgg cgcttctggt tcgaatcatt ccagaaccgg
      121 cagagcgagt tcctcgccgt cggcgcgatg gtcttcctct cgacctacct gcggcagagg
      181 ggcctgcccg agtcgaagcg ggtggacgcg ccgcacagcc aaatcgggaa agtttagtat
      241 ctcttcggcc tatcgattag gcgcggcggt cgatctattg ttggatgtca aaagcgatac
      301 tgccggcgaa gtcggagaac ggcccccacc gctcccacgg ccccccgagg ccggtatgaa
      361 tgcggccctg taggagagtt atcattccgg catgaaggtt cacgtcggca cgtcgggctg
      421 gagctacgcg cactggcacg gcgtgctcta cccgcacggg gcgcacccgc gcgagcgcct
      481 cgactactac ctgctgcgct accgcacggc ggaactcaac agcagttact accgctggcc
      541 cgccgacgcc tccttctcgc gatggcgacg acgcctgccg gaggacttca ccctgtgcgt
      601 gaaggctccg gggctgctca cgcacgtcag ccgcctgtac ggcccggagc gctggctggc
      661 gcgcgttgcg ggaagcctca gccgcctcgg cggcaaactg ggggtgctac tcgtacaact
      721 ctcgccgcgg ttcgcgtacg accacgcccg cctcgcctac ttcctcgacc gcgtgcccgc
      781 cggcctgcgc gtggcggtcg agttccgcca cccgagctgg caccgggaag aggtcttcgg
      841 gctgctgggg cggcacggcg ccggctactg cgtgatgagc ggcgcgcgcc tgccgtgcgt
      901 gctgcgggcg acggcgccgt tcgtctacgt gcggctgcac ggccccgacc acgagcacct
      961 gtacgccggc tcctacccgg agcaagacct gcggtggtgg gcctgtcgga tccgcgagtg
     1021 ggtgagcacg gggcgcgagg tgttcgtcta cttcaacaac gacgggggag ggaacgccgt
     1081 gcgcaacgcc gccgccctga aagcgatctt aggtctgtag acggcctccc cgccgcttcg
     1141 agcggagcgc ccgagacggc gccgtcgtca acgggcggtc tcttgtccgg gaggcgttgc
     1201 gcgcccgtcg cgcgacggtt cgaaacggat gaagatcaag gatgaggggt agaggttacg
     1261 gacatgggtc gttacgaaca gtcggagatc gagtccttca cccggtcagg cacttcctcg
     1321 cgaggcccta aaatcggcaa taatatactt gacgcaccta gccgccgctc cgactcgcgg
     1381 atcctgctaa ttcgaggcct tttcagccgc aattccccaa caggtgcgtc aagtatatta
     1441 ttgccatgta tttgacgaaa agcccgtcag gggtctcgac ggccacgcac tcgcccggca
     1501 ggtagtccgc agacgtggtg aaagtcagga gggagccggc gggcagcccg gccccgatca
     1561 tgctgtcgtc ggagagcctg agcgtgaacg tctcgggtct gttcgaaggt cggaactgga
     1621 ggatgtttgc ggccgattct gacatgaggg tgttcccttc cccgggtctg gattgaggat
     1681 taccgccgca taagaagcaa cctccgttcc tcaatctaac gacgcccctg ggccgcaatc
     1741 ttcacccgtg ccttgccttc gagaatacat cgcattggtg cgcgcggcgc ataacgccgc
     1801 caatcacccg ccgcgcggcc ccgctgccga agggtgacag ccgccgtgtc cgcggtcggg
     1861 tgcattggct tgttcggcgg cggcgcgccg ccaatccagg agccccgcgg cgccgcccgc
     1921 ttcatccggg gtcgggcgac ggcacgcgtg tcacgaagcc gaggatcgtc ccctccggcg
     1981 cctccacgta ggcgacggtc tggccccacg gcatcacgcg cgggggcgtc agcgcggtcg
     2041 ccccggcgcg cactgccgcc tcgaacgcgg ccggcacgtc ctccgcccag aacgcgagct
     2101 ccgtcccgcg cacgcgcccg ctcagcacgg gcgcgtatcc ggcctccagc atcagcgtgc
     2161 cggccacgtg cgacgcgacg gcaacggttt gctccgcgcc gagcagcgcg aagccgagct
     2221 cgcggtcgta gtacgacgcc gtcaggccgg tggcgcgctc gtagaacgcc acggcggcag
     2281 ccacgtcttc cgtgtagatc accgccgtcg cgaactggag cccggtcatc acgtcttcct
     2341 ccgttgcacg agtgagagcc gccgaacgct ccggataacc ggcgagcata agccccgacc
     2401 tttagcaggt gtgccgggag cgagtccggt tcatctggtt gttatgcgcg ccttgccacc
     2461 gatatcgtct gaagtcttgg tccgcccttc tgaggcttcg gcaagttcca aatgcaccgt
     2521 tgtattttca gtcacgctgc caaccgagcc gccccgccag ccccccgctt ccggcaaccc
     2581 tcgcttggat gattcactcg ccccatcgag actaggcacg ccacttcgct ttcgctcact
     2641 ccgcttttgc agcaaaccca gtggcgtgta caacagcagc atcagaagcc tgatgggaag
     2701 aaagaaaaac atcaccggga gcaaggcgag atcgagaaag aaagtgagca tccctttacc
     2761 cacgagccgc catgagctgc cgacctgccc ctcacgaccc gtcgtagtag cactctcgaa
     2821 gaagtggtcg gcgtagtgcg cgacggagcg gtcgagatgc ttattgactc tggtgaatat
     2881 gctgtcttcc ccggtcgtca gggcgatagt gaccacgtca aggttggtgc cgcaggcggg
     2941 acagaacttg tgccggggag aatcatccct cccgcatttc ggacagaaca tgtgttgact
     3001 cctctggcag aatcgatcta catgggcaag tacgatgctg aaaaagcttg gttcaaagag
     3061 aatcgaccac acagcgcata acgcccggcg tcacccgcgc ccctattcaa cattcatgag
     3121 aggcttcgtg gcggggcgtc ggatgcatgc cgttcctatg caaagtcaag actcaagtga
     3181 cgacactact ccgcgccagg ctcatgagca gccgcagctc ggtcgcgccg atctcctc
//
LOCUS       DBNGOH010001136         4201 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4573186, whole genome shotgun sequence.
ACCESSION   DBNGOH010001136 DBNGOH010000000
VERSION     DBNGOH010001136.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4201)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4201)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4201
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4573186"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            198..878
                     /locus_tag="AC159P_22105"
     CDS             198..878
                     /locus_tag="AC159P_22105"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012383.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HIR3698192.1"
                     /translation="MRFTDPGAAGRELAEKVEARGSLAGAVVLAVARGGVPVAAEVAK
                     RLRAPLDVVLVRRLLVPRGPEEPVCAVSVAGALVLDKELAARRAARDDDDKENSALES
                     FLAEALREFEARTRACRGDRPPLDLSRRQVLLVDNGVRTGSTLRAVLRALRTREPARV
                     VAAFPVASVEGRASVAAVADESICLATPEPFGHVGLWYANFERQDDRQIAAALDEAER
                     ARGGLTRA"
     gene            complement(865..2094)
                     /locus_tag="AC159P_22110"
     CDS             complement(865..2094)
                     /locus_tag="AC159P_22110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015181361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3698193.1"
                     /translation="MSSSSESNPTCVSLPAPRRAGRLPLFFVCVLLLVEFLDEIMDGV
                     SGAAWPLIRDDLRLSYAEVGVLLSVPSLVATFVEPVLGILADVWRRRALVLAGGVAFT
                     LAVLSTGLSYSFGALLLASVLFYPASGAFVNFSQAALMDAAPARREQNMVRWTLAGTV
                     GNLVGPLLVGAAVAYGVGWRALFVGLAAFSLLVLALAWRLPFPTPSTSDGAGVGESFR
                     EGVRAARGALRRREVWRWVVLLECGDLTSDVLRGYLALYMVDVVGATGAQAALAVMVW
                     VSLCLAGEFLLLQVLERVGGLRYLRWSTSCVIVLFPLFLLAEGFGAKVALLGALGFVN
                     AGWYSILKAQLYAELPGRSGTVMALDNVAGTVGSLVPLALGAFAQRYGLGAMMWLLLA
                     GPVALLAGLLAVRVRRE"
     gene            2611..>4201
                     /locus_tag="AC159P_22115"
     CDS             2611..>4201
                     /locus_tag="AC159P_22115"
                     /inference="COORDINATES: protein motif:HMM:NF037016.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HIR3698194.1"
                     /translation="MRLSVGLSATLLKNSSLASLFSFILSTALVLIPVLPLTAAGVRA
                     ASAERGDEVAAPPLTRATMPSAALAAPLAPTLTATKVDSFADPDNDGKALPGDTITYT
                     VTITNSGPDDATNVVFNDTIDANTTLVPGSVSTQPIAANDSYNVLGNVRIQPSAASGL
                     LANDCDPDNVGPCSSAGLTASGPTSSANGGDVTVNPNGSFSYNPPAGFEGADSFTYTV
                     TDPQGKTDTATVNFTVNEVVWFVDNTAAAGGDGRLTEPYNSLASLGGADLDEPFDFIF
                     VHEGAGAYGGGIVLEDNQRLIGQGTALDAALTAFGVTTPAHSDARPAATSNPTLANIG
                     GNVITLANGNAVVSVNASATAAASAGISGSGVGGATSVSSVGVSAGGSANGVSLSSLV
                     GSLAMTNSTVSANSSGTAVFISGGATALTFNNTDVSQNGGRVIDIQSRTGGVVSFDAA
                     SSITGTNGTDDAVSLLSNVGATITFNGPVQLNTNASGASGLVADSGTLNMANGGNTVS
                     STGAAAVDIQNMAVNLNFATTF"
BASE COUNT          744 a         1608 c         1333 g          516 t
ORIGIN      
        1 ccgcgaacat gagccggggc aagataacac ggcggcgcgc atgttggcac agggccgaac
       61 cgaacgccgc ctgctttttc gacaccggag gcgcggccca gggcggaaga caaaagccgc
      121 gcctccgcgt cttctggtgt cgaatcgacg tagaggccga gtgcccgtgc cgccgaagca
      181 cgggcggttg acgagcgatg cgattcactg accccggcgc ggcgggccgc gagcttgcgg
      241 agaaggtcga ggcgcgcggc agtcttgcgg gggcggtcgt gctcgcggtc gcgcgcggcg
      301 gcgtgccggt cgcggcggag gtggccaagc gcctgcgcgc gccgctcgat gtcgtgctcg
      361 tccgccgcct gctcgtcccg cgcgggccgg aggagccggt ctgcgccgtg agcgtggcgg
      421 gcgcgctcgt cctcgacaaa gaactcgcgg cccgccgcgc cgcgcgcgac gacgacgaca
      481 aagaaaactc cgcgctcgaa agtttcctcg ccgaagcgct ccgcgaattc gaagcgcgca
      541 cgcgcgcgtg ccgcggggac cgcccgccgc tcgacctctc gcgccgccag gtcctcctcg
      601 tggacaacgg cgtccgcacc ggctcgaccc tgcgcgccgt gctgcgcgcg ctgcggacgc
      661 gcgagcctgc acgcgtcgtc gcggccttcc ccgtcgcttc ggtcgagggc cgcgcgtcgg
      721 tcgccgcggt cgcggacgaa tcgatctgcc tcgccacgcc cgagccgttc ggccacgtcg
      781 gcctctggta cgcgaacttc gaacggcagg acgaccggca gatcgccgcc gcactcgacg
      841 aggccgaacg cgcgcgcggc ggactcactc gcgcctgacg cgcacggcca acagccccgc
      901 gagcagcgcc acaggccccg ccagcaagag ccacatcatc gcgccgagtc cgtatctttg
      961 cgcgaacgcg ccgagcgcga gcgggacgag gctgccgacg gtgcccgcga cgttatccag
     1021 cgccatcacc gtgccgctcc tgccgggcaa ctccgcgtaa agctgcgcct tgaggatcga
     1081 gtaccagccc gcgttgacga agccgagcgc gccgagcagc gcgaccttcg ccccgaaccc
     1141 ttcggcgagc aggaacaaag ggaagaggac gatcacgcac gacgtgctcc agcgcaggta
     1201 gcgcagcccg ccgacgcgct ccaggacttg cagcagcagg aactcgcccg ccagacacag
     1261 ggacacccag accatcaccg cgagcgccgc ctgcgccccc gtcgccccga ccacgtcaac
     1321 catgtagagt gccagataac cgcgcagcac gtccgacgtt aaatcgccgc actccagcag
     1381 gacgacccag cgccagacct cgcgccgcct gagcgcgccg cgcgccgcgc gcacgccctc
     1441 ccgaaaactc tcgccgacgc ccgcgccgtc gctcgtcgaa ggcgtcggga acggcagcct
     1501 ccacgccagc gcgagcacca gcagcgagaa ggccgccagc cccacgaaca gcgcgcgcca
     1561 gcccacgccg taagccaccg ccgcgccgac cagcagcggc ccgaccaggt tgcccaccgt
     1621 ccccgccagc gtccagcgca ccatgttctg ctcgcgccgc gcgggcgccg cgtccatcag
     1681 cgccgcctgc gagaagttca cgaacgcgcc cgacgccggg tagaagagga cggacgccag
     1741 aagcagcgcg ccgaagctgt agctcagccc ggtcgagagc acggcgagcg tgaaggcgac
     1801 gccccccgcg aggacgagcg cgcgccgccg ccacacgtcc gcgaggatgc ccagcacagg
     1861 ctcgacgaag gtcgcgacga gcgacggcac gctcagcagg acgccgacct ccgcgtaaga
     1921 caggcgcagg tcgtcgcgga tgagcggcca cgccgccccg ctcacgccgt ccatgatctc
     1981 gtcgaggaac tcgacgagca gcagcacaca gacgaagaac agcggcagcc gccccgcgcg
     2041 ccgcggcgcg ggcagagaga cacaagtagg attgctttcg gatgacgatg acatcggtga
     2101 ttctccttga acccggaatg cgtcggacgt tttctgtgcg cgagaaaacg tcctccgggc
     2161 ggaggaatca ccgaacgcgc gagggcacgc cacagccttc gcttcgggcg cgcacgcggg
     2221 gcgtgcacga ccgaaggcgc gctgctcggg cttgcccgtc tagcagaggc ttaccaggga
     2281 gggaatttcg agtaatggat gttcatggct tcgagccgtc acgccggagc cgccgggtga
     2341 cgtgcgaaac ttaccacata ggcgcggcgg cgcgaaagtg aactcgccgc gaccgcccgc
     2401 ccggcggcgc gaaagaaaaa ggttgacggg tcgatgccgt taggcttatc gttgcgctcc
     2461 caaatgccag atacaggtat ggccggtctt tagagctttc caccctgctt cagtttgtgc
     2521 gggacatgcc gccccgaacg gttgttaacg ccacgatctg gaagtcattc ccgttgttcg
     2581 aactcaccac cagcaaggag caaaacccac atgcgcctaa gtgtagggct gtctgccacc
     2641 ctcctcaaaa acagttccct ggcctcgctc ttctctttca tcctttcgac agcactcgtc
     2701 ctcatccccg tcctgcccct gaccgccgcc ggcgtgcgcg cggcctcggc agagcgcggg
     2761 gatgaggttg ccgcgccgcc cctcacgcgc gcgaccatgc cgagcgccgc gctcgccgcg
     2821 ccgctcgccc ccacgctcac ggcgacgaag gtggactctt tcgccgaccc cgacaacgac
     2881 ggcaaggccc tgccgggcga caccatcacc tacaccgtca ccatcaccaa cagcggcccg
     2941 gacgacgcca ccaacgtcgt cttcaacgac accatcgacg cgaacacgac gctcgtcccc
     3001 ggctccgtct cgacgcagcc catcgccgcc aacgacagct acaacgtcct cggcaacgtc
     3061 cgcatccagc cgagcgccgc ctcgggcctg ctcgccaacg actgcgaccc cgacaacgtc
     3121 ggcccgtgca gcagcgcggg gctgaccgcc tcggggccga cctcctccgc caacggcggc
     3181 gacgttacgg tcaacccgaa cggcagcttc agctacaacc cgcccgccgg cttcgagggc
     3241 gccgactcgt tcacctacac ggtgaccgac ccgcagggca agaccgacac ggcgaccgtc
     3301 aacttcacgg tcaacgaagt ggtctggttc gtggataaca ccgccgcggc gggcggcgac
     3361 gggcgtctga ccgagcctta caacagcctc gcctcgctcg gcggcgccga cctcgacgag
     3421 cctttcgact tcatcttcgt ccacgagggc gcgggcgcct acggcggcgg catcgtgctc
     3481 gaagacaacc agcgcctcat cggacagggc acggccctcg acgcggcgct cacggccttc
     3541 ggcgtcacca cgcccgcgca cagcgacgcg cgccccgcgg ccaccagcaa cccgacgctc
     3601 gccaacatcg gcggcaacgt catcaccctc gccaacggca acgcggtggt ctccgtgaac
     3661 gcgagcgcga ctgccgccgc ttccgcgggc atctccggca gcggcgtcgg cggcgcgacc
     3721 tccgtcagca gcgtcggcgt cagcgccggc ggctccgcga acggcgtcag cctctccagc
     3781 ctcgtgggca gcctggcgat gaccaacagc acggtctcgg ccaacagctc gggcaccgcc
     3841 gtcttcatca gcggcggcgc gaccgcgctg accttcaaca acacggacgt gagccagaac
     3901 ggcgggcgcg tcatcgacat ccagagccgg acgggcggcg tcgtcagctt cgacgccgct
     3961 tcgagcatca cggggacgaa cggcacggac gacgccgtca gcctgctcag caacgtgggc
     4021 gcgaccatca ccttcaacgg ccccgtccag ctcaacacca acgcgtcggg cgccagcggc
     4081 ctcgtcgccg acagcggcac cctcaacatg gcgaacggcg gcaacaccgt cagctccacg
     4141 ggcgccgccg cggtggacat ccagaacatg gcggtcaacc tcaacttcgc caccaccttc
     4201 t
//
LOCUS       DBNGOH010001137         3362 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_5123753, whole genome shotgun sequence.
ACCESSION   DBNGOH010001137 DBNGOH010000000
VERSION     DBNGOH010001137.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3362)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3362)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3362
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_5123753"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1166
                     /locus_tag="AC159P_22120"
     CDS             <1..1166
                     /locus_tag="AC159P_22120"
                     /inference="COORDINATES: protein motif:HMM:NF051698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HIR3698195.1"
                     /translation="NPSYPDPFAGGTVRQTLPSVRVTDPDLAATYVAVGMLSIERTFL
                     SNLLFTATYDFQREYHRLRTRNINAPLTDEAGAPLRDAANNLIRPFAGQGNLYQYESS
                     GKLDQRQLVFGLFNRLSPKVTLFANYALNKVESDTEGAGTFPANSYDLTGEYGRSTLD
                     IRHRFVMGGTLEAPWGVRFNPFIIIRSGAPFNITLGRDANADTLFTERPAFADAQTLA
                     EDLRSTAFGDFDIDPRPGQTVIPRNYGTGPSFFTVNLRAGKSFSFGGERAAAQADQQQ
                     GGRGGRGGGGGGGGRGGGMGGPGGFGGGRGGGGGRGGGFDGGGGGEGRYNLTVSINVQ
                     NLFNNVNLGTPIGNLSSPLFGNSISAAGGFGFGAGGAGLAGNRRVELQMRFSF"
     gene            complement(1486..2265)
                     /locus_tag="AC159P_22125"
     CDS             complement(1486..2265)
                     /locus_tag="AC159P_22125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HIR3698196.1"
                     /translation="MPYCSRSIRPARARNSLVTSIDVLKLSTAILVVLPLLLCAEVRA
                     QKRRPPGGGRGAAVVVDERLAALRDAPDFSANLLQRMSRGRTVSVLGARRGSDGVVFY
                     RVGVTRRTSGWVQSEAVVRAAGGGDDARLLRLVKGSAGFDRVERACLFLETFPRSPLR
                     PAVLLLLGEAAEEEAATLTRGAVRRLDAREMAAGGAPVHTYFLNYNGLDRFTRRGVRF
                     RFDPAAKRFLYDGAAWREILRRHPRSPEAAEARKRFDVMKK"
     gene            complement(2519..3079)
                     /gene="ruvC"
                     /locus_tag="AC159P_22130"
     CDS             complement(2519..3079)
                     /gene="ruvC"
                     /locus_tag="AC159P_22130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019423669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HIR3698197.1"
                     /translation="MRVLGIDPGSETTGWGVVEGSGRRYGLVAFGTVKAAARERFAAR
                     LLKISEGVEDLLARFQPDVCAVEEAFFAVNAKTALKLGQVRGVVLVAAERARIEIAEY
                     APRLVKQTVVGYGAAEKHQVQEMVRVLLSLKTAPAPLDASDALAVAICHFHHAGLAER
                     AAGGKPAARAKLEALLAANPRRRAPR"
BASE COUNT          541 a         1162 c         1098 g          561 t
ORIGIN      
        1 acaacccgtc gtatcccgat cccttcgcgg gggggacggt gcggcagacg ctgccctcgg
       61 tgcgggtgac ggatcccgat ctggccgcaa cgtacgtcgc cgtcggcatg ctgtcgatcg
      121 agcgcacgtt cctgtcgaac ctgctgttca ccgcgaccta cgattttcag cgcgaatacc
      181 accggctgcg cacgcgcaac atcaacgcgc cgctcacgga cgaggcgggc gcgccgctgc
      241 gcgacgccgc gaacaacctc atccgcccgt tcgcgggaca gggcaacctc taccagtacg
      301 agtcgtcggg caagctcgac cagcgccagc tcgtcttcgg cctcttcaac cgcctcagcc
      361 cgaaggtgac gctgttcgcc aactacgctc tgaacaaggt ggagagcgac accgagggcg
      421 cgggcacgtt ccccgcgaac tcgtatgatc tgacgggcga gtacgggcgc tcgacgctgg
      481 atatccgcca ccgtttcgtc atgggcggca cgctcgaagc gccgtggggc gtgcgcttca
      541 acccgttcat catcatccgc tcgggcgcgc ccttcaacat cacgctcggg cgcgacgcga
      601 acgccgacac gctcttcacc gagcgccccg ctttcgccga cgcgcagacg ctcgccgaag
      661 acctgcgctc gaccgccttc ggcgacttcg acatcgaccc caggcccgga cagaccgtca
      721 tccctcgcaa ctatggcacc ggcccctcct tcttcaccgt caacctgcgc gccggcaagt
      781 ccttcagctt cggcggcgaa agggccgcgg cgcaggctga ccagcagcaa ggtggccgcg
      841 gaggtcgcgg cggcggcggt ggtggtggtg gtcgcggcgg cggcatgggc ggccccggcg
      901 gcttcggcgg cggacgtggt ggcggcggcg gtcgcggggg cggcttcgac ggcggcggcg
      961 gcggcgaagg ccgctacaac ctgaccgtct cgatcaacgt gcagaacctc ttcaacaacg
     1021 tgaacctcgg gacgcccatc ggcaacctca gctcgccgct cttcggcaac tcgatctcgg
     1081 cggcgggcgg cttcggcttc ggcgcgggcg gcgccggcct cgccggcaac cgccgcgtcg
     1141 aactgcaaat gcgcttcagc ttctaagaag gggcgatgag gtgaaaaggg gaagaggcga
     1201 agagggattg aaaacctctt cgcctcttcc ccttttacta cagggtgtga gcttgaatta
     1261 ggaagctaca gcggtggtca ttcggatgcg acctgatgct ggcagtttgc cgccgtgttc
     1321 gcaagaaacg agcgtaagat ggggcatttg gcggaagtcg catacgaatg aaccccgcta
     1381 taggacattt cgtgtgtaga gtcagcggca atgtttgttc tttatctgaa tgtgcccttt
     1441 cccctcctca gaatggtctt ctgctgaatt ctatcttttc ttcctctact ttttcatcac
     1501 gtcgaatcgc ttacgcgcct cggcggcttc ggggctgcgc gggtggcggc gcaggatttc
     1561 gcgccacgcg gcgccgtcgt aaaggaagcg cttcgcggcg gggtcgaaac ggaagcgcac
     1621 gccccgccgc gtgaagcggt cgagtccgtt gtagttcagg aagtaggtgt ggacgggggc
     1681 gccgcccgcc gccatctctc gcgcgtcgag gcggcgcacg gcgccgcgcg tgagcgtcgc
     1741 cgcctcttct tccgcggcct cgccgagcag caacaggacg gcggggcgca ggggcgagcg
     1801 cgggaacgtt tcgaggaaga gacaggcgcg ctcgacgcgg tcgaatcccg cggagccttt
     1861 gacgagacgc agcaggcgcg cgtcgtcgcc gccgcccgcc gcgcgcacga ccgcctcgct
     1921 ctgcacccac ccgctcgtcc gccgcgtcac gccgacgcgg tagaacacga cgccgtcgct
     1981 ccccctccgc gcgccgagca ccgagaccgt ccgccccctg ctcatccgct gcaacaggtt
     2041 cgcggagaag tccggcgcgt cgcgcagcgc cgccagccgc tcgtcaacga cgaccgccgc
     2101 gccgcgcccc ccgcccggcg gacgcctctt ctgcgcccgg acctccgcac agagcaggag
     2161 cggcaggacg acgaggatgg ccgtcgagag tttcagcacg tcgattgatg tgacgagaga
     2221 gttgcgcgcc cgcgcggggc ggatgctccg tgagcagtaa ggcatggtct acggctcctt
     2281 cctggagtgt gtgagaaacg ctcgcgagaa gtgccagtca gcccgccgcg tcacggccag
     2341 atgaagtgcg cgtcgaagtt cgttcgcgtg aagtgcgcgc tgaaccgccg cgccgaagtg
     2401 taggttgaat tcggccagat gtttcgacgc ccggccagca aggcgcgcgc acgcgtcgcc
     2461 gcggcgacgt gtggacttcc ggtcggtgtg tcgtctctgc gcgccccgcg cgcgcgcgtc
     2521 aacgcggggc gcggcggcga ggattcgcgg cgaggagcgc ttcgagcttg gcgcgtgcgg
     2581 cgggcttgcc gccggcggcg cgctcggcga gtcccgcgtg gtggaagtga cagatggcga
     2641 cggcgagcgc gtccgaggcg tcgaggggcg cgggcgccgt cttcagcgag agcagcacgc
     2701 gcaccatctc ctgcacctga tgcttctcgg cggcgccgta gccgaccacc gtctgcttga
     2761 cgagccgcgg cgcgtactcc gcgatctcga tgcgcgcccg ctccgccgcc acgagcacca
     2821 cgccgcgcac ctgcccgagc ttgagcgccg tcttcgcgtt caccgcgaag aaggcttcct
     2881 cgacggcgca cacgtccggc tgaaagcgcg cgagcaggtc ttccacgccc tcgctgatct
     2941 tcaacagccg cgccgcgaaa cgctcgcgcg cggcggcctt caccgtgccg aaggcgacca
     3001 gcccataacg ccgcccgctg ccctcgacca cgccccaccc cgtagtctcg ctgcccggat
     3061 cgatcccaag tacacgcacg cccctttata ccttagaagc ccgcccgcag acagctaaat
     3121 tttagttgca acggaaggta ggcagtaggc ggtaggcggt aggcagtcaa agcaagaggc
     3181 ttgggaggta aagtcccaag cctctcgctt tatcgcttga agagcgaaga ggcttgggac
     3241 tttacctccc aagcctcttt gttctcctgt cttaactgcc tactgccttc tgccttctgc
     3301 ctactttctc acgccatgtc ctcttcgctg atgtcgaagt tggcggagac cttctgcacg
     3361 tc
//
LOCUS       DBNGOH010001138         2824 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_174869, whole genome shotgun sequence.
ACCESSION   DBNGOH010001138 DBNGOH010000000
VERSION     DBNGOH010001138.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2824)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2824)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2824
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_174869"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            305..745
                     /locus_tag="AC159P_22135"
     CDS             305..745
                     /locus_tag="AC159P_22135"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014705.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine decarboxylase family
                     protein"
                     /protein_id="HIR3698198.1"
                     /translation="MRTVGTEWLIDAAGCDASALGDVARLGGVFARAISELGLHPLGE
                     AQWHKFPAPGGVTGFVMLTESHLACHTYPEFGVATFNLYCCRERPEWPWGERLREMLG
                     ARGVKVSRVRRDAPAAESIGGAAPSAVEKEAAAGASAFARKGEA"
     gene            745..2772
                     /locus_tag="AC159P_22140"
     CDS             745..2772
                     /locus_tag="AC159P_22140"
                     /inference="COORDINATES: protein motif:HMM:NF025158.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4178 domain-containing protein"
                     /protein_id="HIR3698199.1"
                     /translation="MAFRAGCPACGAEVIFKSGSSVVVVCEFCHSVVARTDRGVEDMG
                     KVADVVESGSPLEVGLRGAVRNIPFTLTGRAQLAHAAGGMWDEWYAAFEDGRWGWLAE
                     AQGRFYLTFRIPIPNPALLVPFEHLQLGQPVVAIPSQNPPVVAEKGEARMLAAEGEIP
                     YRLVPGEVYQYADLSGAEGIFGTLDYGDSPPNVFVGREVTLAELGIAATGRTREREPR
                     AVTASRLNCPQCAGPLELRAPDKAERVTCPNCGSLLDINQGELRYLKTLQFVGTRPDI
                     APGAVAEFEGQPFTVIGFMTRSVEFEGVRYYWQEYLLYNPMVGFRWLVQSDGHWSYVR
                     PIAPGEVSEQDRKAVYGGRKFKAFQDAAARVDYVAGEFYWKVMVGETVRAVDYVSAPL
                     MLSKEVSGGYGPGKKKKGQIEAEEINWSLGTYLPVKQVEQKFNVELPSPSNVAPNQPF
                     ANKSIYKYWLLFTALAIFVGIGSLLIGSGKTVFQREYTLDPAPAPTPSASQLLGGTFA
                     PAAAEGLENTQVIFTEPFQLRGGENVRVSGAANAENNWIYVAGDLINEETGLVQQFDL
                     PMERYSGVEDGESWSEGSNDKTEVISALPAGTYTMRLEAQWEKWTQPTPPKFSVKVEQ
                     GVAHGFNFFLLLFALAIPPIIVAIRHFSFERRRWADSAFNPYSSGGGDDDE"
BASE COUNT          462 a          925 c          979 g          458 t
ORIGIN      
        1 ggcaaactta aaagccccga aagcaggacg ggcatcatag cacgaaccaa aggactgagg
       61 actgaggagc ggaggactgg gtgaaagcaa ttgacgattg atgagagcga cgctggtcgg
      121 actcgtcacg gttcacgtct ttcactcagt cctcagtcct cagtcctcag tccttggtaa
      181 tcagttcttg aaattttccg ttgcaaacgg aaatcctttc gcctatgctc gccgtcatgc
      241 cctgaaggga caggtcttct cgcccgcgat tgaacttttc cggggagcac ttatcacaca
      301 acgtatgcgg acggtcggga cggaatggct gattgacgcg gcggggtgcg acgcgtcggc
      361 gctcggggac gtggcgcgac tcggcggggt cttcgcgcgc gccatctcgg agctgggctt
      421 gcacccgctc ggcgaggcgc agtggcacaa gttccccgcg ccgggcggcg tgacgggctt
      481 cgtcatgctc accgaatcgc acctcgcctg tcacacctac cccgagttcg gggtcgccac
      541 tttcaacctc tactgctgcc gcgagcggcc cgagtggccg tggggcgagc ggctgcgcga
      601 gatgctcggc gcgcgcggcg tgaaggtgtc gcgcgtgcgg cgcgacgcgc ccgcggcgga
      661 aagtatcggg ggcgcggcgc catcggcggt cgaaaaggaa gccgctgccg gggcgtcggc
      721 cttcgcgcgg aagggggagg cgtgatggcg tttcgtgcgg ggtgtcccgc gtgcggcgcg
      781 gaggtcatct tcaagtccgg ctcctcggtc gtggtcgtgt gcgagttctg ccactcggtc
      841 gtggcgcgca cagaccgcgg cgtcgaggac atgggcaagg tggccgacgt ggtcgagtcc
      901 ggctcgccgc tcgaagtggg cctgcgcggc gcggtcagga acatcccgtt cacgctgacg
      961 ggccgcgcgc agctcgcgca cgcggcgggc ggcatgtggg acgagtggta cgccgccttc
     1021 gaggacggtc gctggggctg gctcgccgag gcgcagggcc ggttctattt gactttccgg
     1081 attccgatcc cgaaccccgc gctgctcgtg cccttcgagc acctccaact cgggcagccg
     1141 gtcgtcgcca tcccttcgca gaacccgccg gtcgtcgccg agaagggcga ggcgcggatg
     1201 ctcgccgccg agggcgagat accttaccgg ctggtgccgg gcgaggtcta ccagtacgca
     1261 gacctgtcgg gcgccgaagg catcttcggc acgctcgact acggcgactc gccgcccaac
     1321 gtcttcgtcg ggcgtgaggt gacgctggcc gaactcggca tcgcggcgac gggccgcacg
     1381 cgcgagcgcg agccgcgcgc cgtcaccgcc tcgcgtctca actgcccgca gtgcgcgggg
     1441 cctttggagc tgcgcgcgcc cgacaaggcc gagcgcgtga cgtgcccgaa ctgcggctct
     1501 ctgctcgaca tcaatcaggg cgagctgcgt tacctgaaga ccttgcagtt cgtcggcacg
     1561 cggcccgaca tcgcgcccgg cgccgtcgca gagttcgagg gccagccctt caccgtcatc
     1621 ggcttcatga cgcgcagcgt cgagttcgaa ggcgtgcgct actattggca ggagtatctg
     1681 ctttacaacc cgatggtcgg cttccggtgg ctcgtccagt cggacgggca ctggagctac
     1741 gtgcggccca tcgcgccggg ggaggtcagc gaacaagacc gcaaggccgt ttacggcggg
     1801 cgcaagttca aggcgtttca ggacgcggcg gcgcgcgtcg attacgtggc gggcgagttc
     1861 tactggaagg tcatggtcgg cgagacggtg cgcgcggtcg attacgtcag cgcgccgctg
     1921 atgctctcga aggaggtctc gggcggctac gggccgggga agaagaagaa ggggcagatc
     1981 gaggccgagg agatcaactg gtcgctcggc acctacctgc cggtcaagca ggtcgagcag
     2041 aagttcaacg tcgagctgcc cagcccgagc aacgtcgcgc ccaaccagcc cttcgccaac
     2101 aagtcgatct acaagtactg gctcctcttc acggcgctcg ccatcttcgt cggcatcggg
     2161 tcgctgctca tcggctcggg caagaccgtc ttccagcgcg agtacacgct cgaccccgcg
     2221 cccgcgccga cgccgagcgc ttcacaactc ttgggcggca cgttcgcgcc cgccgccgcc
     2281 gaagggctgg agaacacgca ggtcatcttc accgagccgt tccagctccg cggcggcgag
     2341 aacgtgcgcg tctcgggcgc ggccaacgcc gagaacaact ggatctacgt cgcgggcgac
     2401 ctcatcaacg aagagaccgg cctcgtccag cagttcgact tgccgatgga gcgctactcc
     2461 ggcgtcgagg acggcgagag ctggagcgag ggttcgaacg acaagacgga ggtcatctcg
     2521 gcgctgcccg cgggcaccta cacgatgcgg ctcgaagcgc agtgggagaa gtggacgcag
     2581 ccgacgccgc cgaagttctc cgtcaaggtc gagcagggcg tcgcgcacgg cttcaacttc
     2641 ttcctcctgc tcttcgcgct cgccatcccg cccatcatcg tcgccatcag gcacttcagc
     2701 ttcgagcgcc gccgctgggc cgacagcgcc ttcaacccct acagctccgg aggcggcgac
     2761 gatgacgaat gacgggcgac ggaaagtcga gggcgcggga agcgcggcgc tcgccctcgc
     2821 gtgc
//
LOCUS       DBNGOH010001139         3183 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4024982, whole genome shotgun sequence.
ACCESSION   DBNGOH010001139 DBNGOH010000000
VERSION     DBNGOH010001139.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3183)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3183)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3183
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4024982"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(201..1946)
                     /locus_tag="AC159P_22145"
     CDS             complement(201..1946)
                     /locus_tag="AC159P_22145"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018761133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase family protein"
                     /protein_id="HIR3698200.1"
                     /translation="MKNPSQIRKNRDQIRRRSFLKLAGGGAAGVIVGAPAVLRFAGSA
                     WAKPHGLSQFTEILPVPPVFDATAGGAFALDMAPSFHQFHAALPPTPTWGYGGAAYLG
                     PTFEAMRGVPFDLTARNMLGPHPLAGVIDTTLHGAADSDRTNPRVSLHLHGGNTEPQS
                     DGHPEATFLPGQERHYHYHNDQEAATLWYHDHALGITRLNVHAGLAGFYLMRDGWDTG
                     RPGNGPGLPTGEYEVPLVIQDRIFNPDGTFEYPLGAGQVWAPEFFGDVAVVNGKVWPN
                     LDVDRGLYRFRIINGSNARVYSLRLSTGQPIHQIGTDGGLLDAPVELGQLVLAPGERA
                     DVLIDFAALRAGTKVLLTNNAATPFPDGPRSVRRGGVPLKQIMQFTVGSDPGHTGPLP
                     TWLRPEMIVPLPAPAVVRNLSLVEIMGEEQPLVALLNNLPWDTTRIETPRVDTVEEWN
                     IINTTGDTHPIHLHLVQFQLAGRQKFDADAYLEAAYPDLMDSGHGGAGPYPAPSAEAF
                     TRGALKGPAANEAGWKDTVRANPGEITRLLVPFGAQAAPGVPCGQSFTGEYVWHCHIL
                     EHEDNEMMLPYEVTP"
     gene            2287..2814
                     /locus_tag="AC159P_22150"
     CDS             2287..2814
                     /locus_tag="AC159P_22150"
                     /inference="COORDINATES: protein motif:HMM:NF012870.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HIR3698201.1"
                     /translation="MFAPQQGNNILDAPSRRSDSRILLIRGLFSRNSPTGASSILLPS
                     LRLKTPKKRLGRQRVEGPPATQPNECRSMDFVADRLAGGHKFRALTIVDNFTRECPAI
                     EVDASLTGARVAAVLEHLRQSRGVPARLKVDNVLPTKASRQPGRSSPAQDSSCLSVPL
                     RAFPAASARARVEDL"
     gene            complement(2905..>3183)
                     /locus_tag="AC159P_22155"
     CDS             complement(2905..>3183)
                     /locus_tag="AC159P_22155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005409655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HIR3698202.1"
                     /translation="AALWMAATVAARMTENSFRRKFDRYIKADPANSDLKRKAYTACA
                     AKMARTVYGLIKHEQFYRAYHEDAVTEPGGRTRSDGPWRPAAQAAATS"
BASE COUNT          508 a         1045 c         1068 g          562 t
ORIGIN      
        1 accgagcgcg ccgacctcca ctactacacc gactaccgcc tcgccgaccg cgacctcctc
       61 ctgttgagcc cgaccgagag ccggcgcatc gtccaccact gaaagcgggg acgggtgacg
      121 ggtgacagga tcagagctgg tctttacccg tcacccgtca cccgtccccg cttgtcaccc
      181 gtcacggctt tccttcctct ctacggcgtc acctcataag gcagcatcat ctcgttgtcc
      241 tcgtgttcga ggatgtggca gtgccagacg tactcgccgg tgaaggactg gccgcagggg
      301 acgccgggcg ccgcctgcgc gccgaagggc acgagcaggc gcgtgatctc gccggggttg
      361 gcgcgcactg tgtccttcca cccggcctcg ttcgcggccg ggcccttcag cgccccgcgc
      421 gtgaaggctt ccgccgaggg ggccgggtac ggcccggccc ccccgtgccc cgagtccatg
      481 agatcggggt aggccgcttc gagataggcg tcggcgtcga acttctgccg tccggcgagc
      541 tggaactgca cgaggtggag gtggatcggg tgcgtgtcgc ccgtcgtgtt gatgatgttc
      601 cactcctcga ccgtgtccac ccgcggcgtc tcgatccgcg tggtgtccca cggcaggttg
      661 ttgagcaggg ccaccagcgg ctgctcctcg cccatgatct cgaccaggct caggttacgg
      721 accacggccg gcgcgggcag cgggacgatc atctccgggc gcagccatgt ggggagcggc
      781 cccgtgtggc cgggatcgct gccgaccgtg aactgcatga tctgcttgag cggcaccccg
      841 ccgcggcgca cgctgcgcgg cccatcggga aaaggcgtcg cggcgttgtt cgtcagcaag
      901 actttcgtcc ccgcccgcag agcggcgaag tctatcagta cgtcggcccg ctcgccgggg
      961 gcgaggacga gctggcctag ctccacgggc gcgtcgagca ggccgccgtc ggtgccgatc
     1021 tggtggatcg gctggccggt cgagagccgc aggctgtaga cgcgcgcgtt cgacccgttg
     1081 atgatgcgga agcgatacaa cccgcggtcc acgtctaggt tcggccagac cttgccgttg
     1141 accaccgcca cgtcgccgaa gaactccggc gcccagacct ggcccgcccc gagcgggtac
     1201 tcaaaggtgc cgtccgggtt gaagatccgg tcctggatga cgagggggac ttcgtactcg
     1261 ccggtcggca gccccggccc gttacccggc cgccccgtgt cccagccgtc gcgcatgagg
     1321 tagaagccgg cgagcccggc gtggacgttg aggcgcgtga tgccgagggc gtggtcgtgg
     1381 taccagaggg tggccgcctc ctggtcgttg tggtagtggt agtgcctctc ctggcccggc
     1441 aggaacgtgg cctcggggtg gccatcggac tgcggctcgg tgttgccgcc gtggaggtgg
     1501 agcgagacgc gcgggttcgt ccggtcggag tcggccgccc catgcagcgt cgtgtcgatg
     1561 acgcctgcga gtgggtgcgg gccgagcatg ttgcgggccg tcaggtcgaa cggcacgcct
     1621 ctcatggcct cgaaggtcgg gccgaggtag gcggccccgc cgtagcccca agtgggtgtc
     1681 ggcggcagcg cggcgtggaa ttggtggaag ctcggcgcca tgtcgagggc gaaggccccg
     1741 ccggccgtgg cgtcgaagac gggcgggacg gggaggatct cggtgaactg cgacaggccg
     1801 tgcggcttgg cccacgccga ccctgcgaac ctgaggacgg ccggggctcc gacgatgacg
     1861 ccggccgcgc cgccgcccgc cagcttcaaa aagcttctcc ttctgatttg atcgcggttc
     1921 ttcctgatct gactgggatt cttcatgaga agacctcctt gggaagggag gccccgggcc
     1981 tgctgggatg ggctagggca ccttctgggc aagtagagac gggggaatta cactccccca
     2041 ggaaaaaacg gctccccgtg ttctttagat tgttggagca accccagtgc cagacccggg
     2101 ggcaggttgt taatgcggat tattgagctg ataagcaggc cgcaagtttt ttgaaggtgg
     2161 gtatcggcgc ccttactggg gacttttacc cggccacgaa gagcgacaat cacccagacc
     2221 agtaaagaag actggccatg aacacagccg cactgttggc cgatccggcc tcgatccaac
     2281 tcgaatatgt tcgcccctca acagggcaat aatatacttg acgcacctag ccgccgctcc
     2341 gactcgcgga tcctgctaat tcgaggcctt ttcagccgca attccccaac aggtgcgtca
     2401 agtatattat tgccctccct caggcttaag acgccgaaga agcggctcgg ccggcagcgg
     2461 gtcgaggggc cgccggccac acagccgaac gagtgccgga gcatggattt tgtcgccgac
     2521 cggctcgccg gcgggcacaa gttccgggcc ttgacgatag tcgataactt tacgcgtgag
     2581 tgcccggcca tcgaggtgga cgcttccttg acgggcgcac gtgtcgcggc ggtgctggag
     2641 catctgaggc agtcgcgagg agtgccggcg aggctcaaag ttgataacgt actacctaca
     2701 aaagcaagtc ggcagcccgg ccgcagttct cctgcgcagg atagttcctg cttgtcagtg
     2761 ccccttcgag cgttcccggc ggcttccgcc cgcgcgaggg tagaagacct ctgaccatag
     2821 tgacacaagg tcggcactcg gaacgcgccg ccttcactct actcaacaca aagtatctgc
     2881 cccggcggaa gaccctatca ttatctacga ggtcgcggcc gcctgtgcgg ccggcctcca
     2941 cggcccatcc gagcgagttc ttccgccagg ttccgtcacc gcgtcctcgt gataggcgcg
     3001 gtagaactgt tcgtgcttga tcagcccgta gaccgtgcgc gccatcttcg ccgcgcacgc
     3061 cgtgtacgcc ttccgcttga ggtcggagtt cgccgggtcg gccttgatgt agcggtcgaa
     3121 cttccggcgg aagctgttct ccgtcatccg cgcggcgacc gtcgccgcca tccacagcgc
     3181 cgc
//
LOCUS       DBNGOH010001140         2367 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2007969, whole genome shotgun sequence.
ACCESSION   DBNGOH010001140 DBNGOH010000000
VERSION     DBNGOH010001140.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2367)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2367)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2367
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2007969"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1923
                     /locus_tag="AC159P_22160"
     CDS             <1..1923
                     /locus_tag="AC159P_22160"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003470709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 97 protein"
                     /protein_id="HIR3698203.1"
                     /translation="VVLCAGIAGAATETRATRVAGPGGRLAVYFRLDAAGVPRYAIRL
                     DGKPVLRESRLGLVRDDEDFSRGLRLVSASRVVPVRERYEILTAKRRVNDYRANRMTV
                     RLQTAAGRRMEIVFQVSDDGVAFRYRFPETSAETRRLVEEVTSFNFPPGTRAWLQPMS
                     VAKSGWEKVNPCYEEFYEKEIAAGTPSPLGAGWVYPALFRTGDTWVLVSESALARNYS
                     GTRLRHESPGGEYAVGFPDPRENFRGGPVNPESKLPWLTPWRVVVVGSLKTIAESTLG
                     IDLADRPAARYRNAAVKPGKASWSWPLLGDRQTVYEVQKRFIDYAAEMRWSYCLIDGH
                     WDTQIGYEKTKELIDYARGKGVRVLLWYNSAGDWNTAPQTPRDLMLTRESRVREFERL
                     KAMGVAGLKIDFFGGDGQSMIAYYHDILEDAAPYGFLINFHGATLPRGWQRTYPHLMT
                     AEAIKGLEYVTFEQKNADEEPTHAAMLPFTRNVFDPMDFTPVVLDRIQRIERRTTSAF
                     ELALSVLFTSGIQHYAEIPEGMRKAPPYVRDFLKAVPGVWDDTKFLEGYPGKFVALAR
                     HGGGRWYVAGINGEATPKKLTLDLAELRARGTGSLIADGEGGNLSFRQETVRLAADRK
                     LEITLPPRGGFVLALE"
     gene            2013..>2367
                     /locus_tag="AC159P_22165"
     CDS             2013..>2367
                     /locus_tag="AC159P_22165"
                     /inference="COORDINATES: protein motif:HMM:NF016303.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF542 domain-containing protein"
                     /protein_id="HIR3698204.1"
                     /translation="MTLCAEKTVREYAVETPHAARVFEKLKIDYCCGGGRPLGEAARL
                     AGVPLEEVERLLEQAGEASGGVPAGAPSGTLTELIDYILDKHHAFTRDEMERIDALAE
                     KVASKHGGNHPELASV"
BASE COUNT          393 a          785 c          831 g          358 t
ORIGIN      
        1 gtcgtgctgt gtgcgggcat cgccggcgcg gccaccgaga cgcgggcgac ccgtgttgcc
       61 ggccccggcg ggcggctcgc ggtctacttc cgtctcgacg ccgcgggcgt gccgcgttac
      121 gccatccgcc tcgacggcaa gcccgtgctc cgcgagtcgc gcctcggcct cgtgcgcgat
      181 gacgaagact tctcgcgcgg gctgcggctc gtgtcggcgt cgcgcgtcgt gcccgtgcgg
      241 gagaggtacg aaatcctcac ggccaagcgg cgcgtgaacg actaccgcgc gaaccggatg
      301 acggttcgtt tgcagaccgc cgcgggccgg agaatggaga tcgtttttca ggtctcggac
      361 gacggcgtgg ccttccgcta ccgctttccg gagacgagcg ccgagacgcg acggctcgtc
      421 gaggaggtca cgtcgttcaa cttccctccg ggcacgcggg cgtggttgca gccgatgtct
      481 gtggccaaga gcggttggga gaaggtgaat ccctgttacg aggagtttta cgaaaaagag
      541 atcgccgccg ggacgccgtc gcccctcggc gcgggctggg tctacccggc gctcttccgc
      601 acgggcgaca cctgggtgct cgtgagcgag agcgcgctcg cgcgcaacta cagcggcacg
      661 cggctgcgcc acgagtcgcc gggcggcgag tacgcggtcg gctttcccga cccgcgcgag
      721 aattttcggg gcggcccggt caaccccgaa tcgaagctcc cgtggctgac gccgtggcgc
      781 gtcgtcgtgg tcggcagtct gaagacaatc gccgagtcca cgctcggcat cgacctcgcc
      841 gacaggcccg cggcgcgcta caggaacgcg gcggtcaagc cgggcaaggc gtcgtggagc
      901 tggccgctgc tcggcgaccg gcagaccgtc tacgaggtgc agaagcgttt catcgactac
      961 gccgccgaga tgcgttggag ctactgcctg atcgacggcc actgggacac gcagatcggc
     1021 tacgagaaga cgaaggagct gatcgactac gcgcgcggca agggcgtccg ggtcctgctc
     1081 tggtacaact ccgcgggcga ctggaacacg gccccgcaga cgccgcgcga cctgatgctg
     1141 acgcgcgaga gccgcgtgcg cgagttcgag cggctgaagg cgatgggcgt cgccggtctc
     1201 aagatcgact tcttcggcgg cgacggacag tcgatgatcg cctactatca cgacatcctc
     1261 gaggacgccg cgccctacgg cttcctcatc aacttccacg gcgcgacgct gccgcgcggc
     1321 tggcagcgca cctacccgca cctgatgacg gcggaagcga tcaagggtct cgaatacgtc
     1381 accttcgaac agaagaacgc cgacgaggag ccgacgcacg cggcgatgct gccgttcacg
     1441 cggaacgtct tcgacccgat ggacttcacg cccgtcgtgc tcgaccgcat ccagcgcatc
     1501 gagcggcgca cgacgagcgc cttcgagctg gcgctctcgg tcttgttcac gtcgggcatc
     1561 cagcactacg cggagattcc cgaagggatg cggaaggcgc cgccctacgt ccgcgacttc
     1621 ctcaaggcgg tgccgggcgt gtgggacgac acgaagttcc tcgaaggcta ccccggcaag
     1681 ttcgtcgcgc tcgcgcggca cggcgggggc cgctggtacg tcgcgggcat caatggcgag
     1741 gcgaccccga aaaagctgac gctcgacctc gcggagctgc gcgcccgcgg caccggctcg
     1801 ctcatcgcgg acggcgaggg cggcaacctc tcgttccggc aagagacggt gcgtctcgcc
     1861 gccgaccgga agctcgaaat cacactgccg ccgcgcggcg gtttcgtctt ggcgctggag
     1921 tgagacggca aatttcccat ccgtgactta tgtcactgcg cgggcgcgcg ggtcggcctc
     1981 agaatgatgg gcgagaagga ggtttcactc aaatgactct atgtgccgag aagacagtcc
     2041 gcgagtatgc ggtcgagacg ccgcacgccg cgcgcgtctt cgagaagctg aagatcgatt
     2101 actgctgcgg cggggggcgc ccgctcggcg aggcagcgcg actggcaggc gtcccgttgg
     2161 aggaagtcga gcgtctgctc gaacaggccg gggaagcctc cggcggggtg ccggcgggcg
     2221 caccgtcggg cacgctgacg gagctgatcg attacatcct cgacaagcac cacgccttca
     2281 cgcgcgacga gatggagcgc atcgacgcgc tcgccgagaa ggtcgcttcg aagcacggcg
     2341 ggaatcaccc cgagctggcg agcgtcc
//
LOCUS       DBNGOH010001141         4271 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_5124651, whole genome shotgun sequence.
ACCESSION   DBNGOH010001141 DBNGOH010000000
VERSION     DBNGOH010001141.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4271)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4271)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4271
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_5124651"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2070
                     /locus_tag="AC159P_22170"
     CDS             <1..2070
                     /locus_tag="AC159P_22170"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR3698205.1"
                     /translation="ETLRWRMAGGTVSLSEALDVATQTATALAAAHAAGLVHRDIKPE
                     NIMLRSDGYVKVLDFGLVKLTERRDKASEASTLVHTDAGMVMGTVAYMSPEQARGLEV
                     DARTDTFSLGVVLYEMLAGRTPFAGETTSDVIAAILKTEPTPPSDLNREVPAELEGIV
                     SRMLEKDRAGRYQTAEELLADLRQLQTRLDFGAEAERELVTRHSGGPSAIQAETQSDG
                     AAARTSKAEARNSIAVLSFANLSAEAENEHFCDGLAEELLNTLAKIEGLKVAARTSAF
                     SFKGKDAKVSEIGRALGVRTVLEGSVRKSGNRLRITVQLVNAADGYHLWSERYDRELQ
                     DIFDVQDEITLSVVDALKVKLFGVEKAAVLKRYTDNTEAYQLYLKGLYFLEKFTEDGF
                     KKSIECFHQAVQMEPNYAQAYAGLAACYSLLGLTHAPSDEMYPQAKEAALKALELDNT
                     LAEAHCALGGVKLFYDWDWPAAKREAQLAIELNANFADAHVLYGYYQLVMGRLRECIA
                     EIKLAQELNPLSRFVNSYLGWVLYFARQYDQAAEQCLKTHELDPDYTPLRSLLGRAYL
                     MKGMYREAITELEKDLGLSGEPPEALANLGHAHAMSGNRDEAQKAVDKLKRLFVQREV
                     NIDPTLIAIVYAGLDERDQAFEWLHQAYECRALNLLWLKVEPVWDSLRSDSRFTDLLR
                     RMNLAPG"
     gene            complement(2298..3161)
                     /locus_tag="AC159P_22175"
     CDS             complement(2298..3161)
                     /locus_tag="AC159P_22175"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 family peptidase"
                     /protein_id="HIR3698206.1"
                     /translation="MRRNLCLLALLAAVVTSALLVTPARVRAITYGFVDTTNAYPNVG
                     AFTVQTPGGEIFPLCSGTLIAPNVFLTASHCTAYYTNVLAPLGYTAYVSFDKSIPFGD
                     LTSQQTVLFPVADVVTNPNYSQRQSDSGDIGVLILESDVNGITPATLPTCGLLDQLSA
                     RSGLKGAAFTAVGYGVQNRVVGGGVPYFQDVNPIPRMFAFSSYNALNKGYLRLSQNPA
                     TSNGGACYGDSGGPNFLTVDGQQILVATTVTGDAVCRATNVVYRLDTASAQAFFDYVN
                     ATYGTQIPTSC"
     gene            3484..>4271
                     /locus_tag="AC159P_22180"
     CDS             3484..>4271
                     /locus_tag="AC159P_22180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_089339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS701 family transposase"
                     /protein_id="HIR3698207.1"
                     /translation="MSREVADSKAAVEEAERWAQGLGAVAERIQGRFPRSEPRERATA
                     YLRGLVSSVERKNGWQLAEAAGDKKPYGVQHLLGRAEWSADEVGDDLRFYVTEHLGDP
                     GAVLVVDETGFLKKGSKSAGVARQYSGTAGRVENCQIGVFLAYATAEGRTFLDRELYL
                     PKEWAEDAGRRAGAGVPEEVKFATKPQLARQMIGRALGAGVPFGWVTADSVYGNDWRM
                     RAWLEERQISYVLGVTAPYRIFTGSVREWASAVVRRLAKKQWRRL"
BASE COUNT          870 a         1201 c         1435 g          765 t
ORIGIN      
        1 gaaacgctcc gctggcgcat ggcgggcgga accgtgagtc tgagcgaagc gctcgacgtg
       61 gccacgcaaa ctgcaaccgc cctggctgcg gcgcacgcgg cggggctcgt ccaccgggat
      121 atcaaaccgg aaaacatcat gctcaggagt gacgggtacg tcaaagtcct ggatttcggt
      181 ctggtgaaac tgacggagcg ccgggacaaa gcctcggaag cctcaacgct ggtccatacg
      241 gacgcgggca tggtgatggg gaccgtcgcc tacatgtcgc ccgaacaggc gcgcgggctg
      301 gaggtcgacg cgcggacgga caccttcagc cttggtgtcg tgctgtacga gatgctcgcc
      361 ggcagaacgc ccttcgccgg cgagacgacg agcgacgtga tcgccgccat cctgaagacc
      421 gagcccacgc cgccgagcga tttgaacagg gaggtccccg ccgaactgga aggaatagtg
      481 agcaggatgc tggagaaaga ccgcgccggg cgctatcaaa ctgccgaaga gttgctcgcc
      541 gacttgcggc agttgcagac gcgcctcgac ttcggagcgg aagccgagcg tgagcttgtc
      601 acccgtcatt ccggcggccc gtcggccatc caggctgaaa ctcaaagcga cggcgcggcg
      661 gcgagaacct ccaaggcgga ggcgcgcaat tcaatcgccg tgctgtcatt cgccaacctg
      721 agcgcggagg ccgagaacga acacttctgc gacgggctgg ccgaagagtt gttgaatacg
      781 ctggcgaaga tcgaaggctt aaaggtggcg gcgcgcacgt cggccttctc gttcaagggt
      841 aaggatgcca aggtcagcga gatcgggcgc gctttaggcg tgcggacggt gctcgaaggc
      901 agcgtgagga agtctggtaa ccgcctgcgg atcacggtgc agttggtcaa cgccgcggac
      961 ggctaccacc tgtggtcgga gcgttacgac cgcgagctgc aagacatctt cgacgtgcag
     1021 gacgagatca cgctctctgt tgtagatgcg ctgaaggtga agctgttcgg cgtggaaaag
     1081 gcggcggtgc taaagcgtta caccgacaac acagaggctt atcaacttta tctcaagggg
     1141 ttgtatttcc tagagaagtt tactgaagat ggattcaaga aaagcatcga gtgctttcac
     1201 caggcggtcc agatggagcc gaactacgcc caggcttacg ccgggttggc cgcttgctac
     1261 agcctgttgg gtctcacgca tgcgccttcc gatgagatgt acccgcaagc caaagaggcg
     1321 gcattgaaag ccctggagtt agacaacaca ctggccgaag cacactgtgc gctgggaggt
     1381 gtaaagcttt tttacgactg ggactggccg gcggctaagc gagaggcgca gcttgccatt
     1441 gagcttaacg cgaactttgc ggacgcccac gtcctctacg gctactatca gttggtgatg
     1501 ggacggcttc gggaatgcat cgcagaaatc aagctggcgc aagagcttaa tccgctctcg
     1561 cgcttcgtca actcatatct gggatgggta ttatacttcg cgcgacagta cgatcaggcg
     1621 gcggaacaat gcctcaagac acatgagctg gacccggact ataccccgct gcgctcattg
     1681 ctcgggaggg cttatctgat gaaggggatg taccgcgagg ccatcacaga attagaaaag
     1741 gacttgggcc tttcagggga gcccccggaa gcattagcaa acctcggtca cgcccacgcg
     1801 atgtccggga acagagatga ggcgcagaaa gcggtggaca aattgaagcg gctttttgtg
     1861 cagcgcgaag taaatattga ccccacgctc atcgccatcg tctacgcagg tctggatgag
     1921 cgagatcagg ctttcgaatg gctgcatcag gcgtatgagt gccgtgcgct gaatctgctc
     1981 tggcttaaag ttgagccggt gtgggatagc ctgcgttctg attcgcgatt cacagacctg
     2041 ctgcggcgca tgaacctcgc gccgggatga aatgaaattc tcatgacagc actttatcca
     2101 ataactaaaa ggagtctctc cgtgcgccgt caccaaaagt gcgggagagc ttttacgggt
     2161 tcacccgccc ttttgatgac gcggtgcggc aggtgtcaaa tttcacgtcg tgctgagagg
     2221 cggggctcta cgtcgccccc ggccctcgtg tgtgagtggt ctacccgggg ccgggggcgc
     2281 cgctggtgtt tgcaacgcta acaggaggtc gggatctgcg tgccgtacgt ggcgttcacg
     2341 taatcgaaga acgcttgcgc cgaggcggtg tcgagccggt acaccacgtt cgtcgcccgg
     2401 cacacggcgt cgccagtgac cgtcgtcgcc acgagaatct gctgcccgtc gacggtgagg
     2461 aagttcggcc cgcccgaatc cccgtaacat gccccgccgt tacttgtggc agggttttgc
     2521 gacagccgta ggtacccttt gttgagggcg ttgtacgaag agaaggcaaa catcctgggt
     2581 atggggttaa cgtcctggaa gtaaggcacc ccgccgccga ccacccgatt ctgcaccccg
     2641 tacccgactg cggtgaaggc ggcgcccttc aacccactcc gggccgagag ctggtccagc
     2701 agcccgcaag tcggcagcgt cgcgggggtg atgccgttga cgtccgactc gagtatcaag
     2761 accccgatgt cgcccgagtc gctctggcgc tggctgtagt tggggttggt gacgacatcc
     2821 gccaccggga acagcacggt ctgctggctg gtcagatccc cgaaagggat cgacttgtca
     2881 aagctcacgt acgcggtgta gcccagcggg gcgaggacgt tggtgtagta ggccgtgcag
     2941 tggctcgccg tcaggaacac gttcggcgcg atcaatgtcc cggagcagag tgggaagatc
     3001 tctccgcccg gcgtctggac ggtgaacgcg ccgacgttcg ggtaagcgtt ggtggtgtcg
     3061 acgaagccgt aggtgatagc ccggacgcgg gcgggcgtga cgagcagggc gctggtgacc
     3121 accgcggcga gcaaggctag aaggcagaga ttgcgacgca tcacgaggtt ctcctttatt
     3181 tttcggctga gcgttcgctg tccggttgag tcggggattc ggcagagcac acgatatacc
     3241 tcccggctca cgtgcagcaa gcgaagcctc tacacgtctt gccctgacga ccagtgtcaa
     3301 agagctgtgg tgctggatga tcttttctaa gctgatcgcg gtcgggtcgg cgaggagggt
     3361 cgaggtcagc atcggcggag tctaaccgcc ttatcaaaca gcaccaaaat cgcggaagac
     3421 ccgaacctat tactacagtt gtagatatag aactgctatg ctctccagcc agcggagggc
     3481 atgatgagcc gggaagttgc cgatagcaaa gcagcggtcg aagaagccga gcgttgggca
     3541 caaggactcg gggcggtcgc cgagcgcatc caagggcgct tcccgcgctc cgaaccgcgc
     3601 gagcgtgcca cggcctacct gcgcgggctc gtctcgtcgg tcgagcgcaa gaacggctgg
     3661 cagttagccg aagccgctgg agataagaag ccatacggcg tacaacacct gctcgggcgc
     3721 gccgagtgga gtgcggacga ggtgggtgat gacctgcgct tttacgtcac cgagcacctc
     3781 ggagaccccg gcgccgtact tgtcgttgac gagacaggtt tcctcaagaa aggcagcaag
     3841 agcgcggggg tggcccgcca gtacagcggc acggccgggc gggtcgagaa ctgccagatc
     3901 ggcgtcttcc tcgcctacgc gacagcggaa gggcgcacct tcctggatcg tgagttgtac
     3961 ttgccgaagg agtgggctga ggacgccggg cggagggccg gggcgggcgt ccccgaagag
     4021 gtgaagttcg cgaccaagcc gcaactggcg cggcagatga tcgggcgcgc cctgggagcg
     4081 ggtgtgccgt tcggctgggt caccgcagac tccgtgtacg gcaacgactg gcggatgcgg
     4141 gcgtggctcg aagagagaca gatcagctat gtgctcgggg tgacggcgcc gtaccgcatc
     4201 ttcacgggca gcgtgcgcga gtgggcttcg gcggtcgtcc gaaggctggc gaagaaacag
     4261 tggcggcggc t
//
LOCUS       DBNGOH010001142         4720 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1826621, whole genome shotgun sequence.
ACCESSION   DBNGOH010001142 DBNGOH010000000
VERSION     DBNGOH010001142.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4720)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4720)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4720
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1826621"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..393)
                     /locus_tag="AC159P_22185"
     CDS             complement(<1..393)
                     /locus_tag="AC159P_22185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3698208.1"
                     /translation="MNRLITAAVALPVLIASILYPPLKYLFVGIAVAAIVVALYEFWF
                     LAKRVGAKPDIVVGYAATAALLVAFFYNRLEWLVVIVPGLVVAALAAEMLRGAPFDKM
                     ILSVGSTVLGVLYVALLGGHMIALRTDFA"
     gene            complement(517..1311)
                     /locus_tag="AC159P_22190"
     CDS             complement(517..1311)
                     /locus_tag="AC159P_22190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HIR3698209.1"
                     /translation="MALGDFAEIVERGSRDEALLARVEWERLPRHVAVIMDGNGRWAA
                     RRGQPRVAGHRAGVEAVRAAVDTGARLGLRALTLYAFSTENWKRPRLEVDALMRMLKR
                     YLRLELEEIHRQNIRFQPIGRIEGLAESVRRELQRAVERTATNTGMVLSVALNYGGRA
                     EIVDAVRAAAAELTARGTSPAEMTDADIERHLYTKGLPELDLLVRTSGEMRISNFLLW
                     QAAYCEIHVTETLWPDFRRTHLLEAVLDFQGRDRRFGGLKLVANAK"
     gene            complement(1430..2194)
                     /gene="truA"
                     /locus_tag="AC159P_22195"
     CDS             complement(1430..2194)
                     /gene="truA"
                     /locus_tag="AC159P_22195"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HIR3698210.1"
                     /translation="MNYRFTIQYDGTELHGWQAQDGRRTVQGELERALALLEGEAVVL
                     HGAGRTDAGVHAEGQVASAHLRRREWSAEKLRAAVNGNVGTDVRVIDVVEAPDDFHAR
                     YSARGKTYRYAVFNERFQSPFWSRYALHEARHLDVGAMRRAAAHFVGEHDWTAFSAAQ
                     SDVKTRVRRVTRLDISERVCERGRGRVVEIEAGAEGFLRYMVRTMAGALLAAGRGELK
                     AQDIARAIETGERPHLIVTAPAHGLTLVKVEYGAES"
     gene            complement(2380..2967)
                     /gene="pyrE"
                     /locus_tag="AC159P_22200"
     CDS             complement(2380..2967)
                     /gene="pyrE"
                     /locus_tag="AC159P_22200"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0019856 - pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HIR3698211.1"
                     /translation="MREDEVIDRFRRTQALLEGHFVLTSGLHSTNYLQCALVLQHPAE
                     AEAFGRAIAERFRGQQIETVAAPAIGGIIIGWEVARALGVRSVWTEREHGRMTLRRGF
                     NVRDGERVVVVEDVITTGGSTRETIEALRDLGANVVGAASIIDRSGGRADVGAPRHAL
                     ATLDVPTHAPEACPLCAQGIPAIKPGSRKEAGSGQ"
     gene            3012..3899
                     /locus_tag="AC159P_22205"
     CDS             3012..3899
                     /locus_tag="AC159P_22205"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HIR3698212.1"
                     /translation="MQSFRNLVPDSTLIHEAIQLLRRQGGRASAVEVADTVLQLPELD
                     PPLAATLVAELIKDDWRLRVDDGTHDIELLCEDDELRSLADTDYVVFDVETTGPKTPP
                     GRIMELGACRVSGGRIVGEFQTLVNPRAPIPPFISQLTGISDTMVSAAPLFAEIAPAW
                     LEFADRAVLVAHNAPFDIRFINHELSLVYPGRRMSNAHICTVSLARRAVPELPSHRLH
                     LLAEHFAVHFPRRHRAGDDARATAEVFIRLLERLRRDGVHDLASARQYKRPEANGRKS
                     EAKGQKTGTVGHTVRTSPV"
     gene            complement(3947..4630)
                     /locus_tag="AC159P_22210"
     CDS             complement(3947..4630)
                     /locus_tag="AC159P_22210"
                     /inference="COORDINATES: protein motif:HMM:NF020893.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HIR3698213.1"
                     /translation="MKWLWRKITNGLDSVSQFLVAFGPLGLFAIALLDSALIPLPGGP
                     DAVMILLSTQRPAWMPVYALAATAGSVIGCVILYRISRRAGRRALERFPPSKQARVKE
                     LVDRYDVLSVLVASVLPPPFPFKLFVITAGVFRLSLVRFAVAVGIGRAFRFLLEGFIA
                     VRYGERAKEVLARNYPAVGLGVAVLIVAVFVIRNLLKRRRTKIGAGGRGPGAGEEQRA
                     GGKSLSEEG"
BASE COUNT          806 a         1730 c         1457 g          727 t
ORIGIN      
        1 cgcgaagtcc gtccgcaacg cgatcatgtg cccgcccagc agcgccacgt agagcacgcc
       61 gagcacggtc gagccgaccg acagaatcat cttgtcgaac ggcgcgcccc gcagcatctc
      121 cgccgccagc gccgccacga ccagccccgg cacgatgacg accagccact ccaacctgtt
      181 gtagaagaac gcgacgagca gcgcggcggt cgccgcgtac cccacgacga tgtccggctt
      241 cgcccccacg cgcttcgcca gaaaccagaa ctcgtacagc gccaccacga tggccgcgac
      301 cgcgatgccg acgaacagat acttcagcgg cggataaaga atcgaagcga tgaggacggg
      361 cagggcgacg gcggcggtga tgagacggtt catggggagc tgttagctgt tagctgttag
      421 ctaaaagcct tcttagcttc gtgcatttca agagcgttcg gaagaggtga tgcggacgtt
      481 tagaaaacaa atacgctaac agccaatagc taacagctat ttcgcgtttg ccaccagctt
      541 gaggccgccg aagcggcggt cgcgtccctg aaagtcaagg acggcttcga gcaggtgcgt
      601 gcggcggaag tcgggccaga gcgtctcggt gacgtgaatc tcgcagtagg ccgcctgcca
      661 caaaaggaag ttcgagatgc gcatctcgcc gctcgtccgc acgagcaggt cgagttcggg
      721 cagacccttc gtgtagaggt ggcgctcgat gtcggcgtcg gtcatctcgg cgggcgacgt
      781 gccccgcgcc gtcagctcgg cggcagccgc gcgcaccgcg tccacgatct ccgcgcgccc
      841 gccgtagttg agcgcgacgc tcaagaccat ccctgtgttc gtcgccgtcc gctcgaccgc
      901 gcgctggagt tcgcgccgca cgctctcggc cagaccctcg atgcgaccga tgggctggaa
      961 gcggatgttc tgccggtgaa tctcttcgag ttcgagacgc agataccgct tcagcatccg
     1021 catcagagcg tccacttcga gccgcgggcg cttccagttc tccgtcgaga aggcgtagag
     1081 cgtgagcgcc cgaaggccca accgcgcgcc cgtgtccacc gccgcgcgca cggcctcgac
     1141 gcccgcgcgg tggcccgcca cgcgcggctg cccgcgccgc gccgcccagc gcccgttgcc
     1201 gtccatgatg acggccacgt ggcgcggcaa acgctcccac tccacgcgcg ccagcagcgc
     1261 ctcgtcgcgc gacccacgct ccacgatctc tgcgaaatca ccaagcgcca aaggctttcc
     1321 ccctcgcacc acaccaagac cctgcggcaa accccgcggc atcttataac gcaaactagt
     1381 tcgaagtaca aagtacatcg gagagttcaa gggccggagt gcggcgcgct taactttccg
     1441 ctccgtattc gaccttcacc aacgtcaggc cgtgcgcggg cgcggtgacg atgaggtgcg
     1501 gcctctcgcc cgtctcgatg gcgcgcgcga tgtcctgcgc cttcagctcg ccgcgcccgg
     1561 cggccagcag cgcgcccgcc atcgtccgta ccatgtagcg caggaagcct tcggcgccgg
     1621 cctcgatttc gacgacgcga ccgcgaccgc gctcgcacac gcgctccgag atgtcgagcc
     1681 gcgtcacgcg gcgcacgcgc gtcttcacgt ccgactgcgc cgccgagaag gccgtccagt
     1741 cgtgttctcc gacgaagtgg gccgcggccc gcctcatcgc tcccacgtcc aagtgccgcg
     1801 cctcgtgcag cgcatagcgc gaccagaagg gcgactggaa gcgctcgttg aagacggcgt
     1861 agcggtacgt cttgccgcgc gccgagtagc gcgcgtggaa atcgtccggc gcttcgacaa
     1921 cgtcgatgac gcgcacgtcc gtgccgacgt tcccgttgac cgccgcacgc agtttctccg
     1981 ccgaccactc gcgccgccgt agatgcgcgc tcgccacctg cccctccgcg tgcacacccg
     2041 cgtccgtccg ccccgccccg tgcagcacga ccgcctcgcc ttccaacaac gcgagcgcgc
     2101 gctccagctc gccctgcacc gtgcgccgcc cgtcctgcgc ctgccacccg tgcagctccg
     2161 tcccgtcgta ttgaatcgtg aagcggtaat tcataagagc ggcaggcagc gggcagcaag
     2221 cagcaggcag ttaaagacag gagcagcggg cggcgggtgg caagccacag acaacgagag
     2281 cgattcgagt atttgtaaat tcaagttcga gatcggcgcc cgccttatcc aatcgagaat
     2341 caacagtcag caatcgacaa cgctcttact gcctgctgcc tactgcccgc tgcctgcttc
     2401 tttcctgctt ccgggtttga ttgccgggat gccttgcgca cagagggggc aggcttcggg
     2461 ggcgtgtgtg ggaacgtcga gggtggcgag ggcgtggcgc ggggcgccca cgtcggcgcg
     2521 accgccggag cggtcgatga tggaggcggc gccgacgacg ttcgcgccca gatcgcggag
     2581 cgcttcgatg gtctcgcgcg tcgagccgcc ggtcgtgatg acgtcttcga cgacgacgac
     2641 cctctcgccg tcgcggacgt tgaagccgcg ccgcagcgtc attcgcccgt gctcgcgctc
     2701 tgtccagacc gagcggacgc cgagcgcgcg cgccacctcc cacccgatga tgatgccgcc
     2761 gatggcgggc gcggccaccg tctcgatctg ctggccgcgg aagcgttcgg cgatggcgcg
     2821 tccgaaggct tcggcctccg ccgggtgttg caggacgagc gcgcattgca ggtagttggt
     2881 gctgtgcagc ccgctcgtca aaacgaagtg gccttcgagc agcgcttgcg tccgcctgaa
     2941 cctgtcgatc acctcgtcct ctcgcatctc gcccttccct ttcgaaacct gcgtgttaca
     3001 atgcgcttcc catgcagtcc ttccggaacc ttgtcccaga ctctaccctc atccacgagg
     3061 cgatacaact tcttcgccgg cagggcggtc gcgcctccgc ggtcgaggtc gccgacacgg
     3121 tgctgcaact gcccgagctg gacccgccgc tcgccgccac gctcgtcgcc gagctgatca
     3181 aggacgactg gcggctgcgc gtggacgacg gcacgcacga catcgagctg ctctgcgagg
     3241 acgacgagct gcgctctctg gccgacaccg attacgtcgt cttcgacgtg gagacgaccg
     3301 ggccgaagac accgcccggc aggatcatgg aactcggcgc gtgcagggtc agcggcggtc
     3361 gcatcgtcgg cgagttccag acgctcgtca acccgcgcgc gccgatcccg cccttcatct
     3421 cgcagctcac gggcatctcg gacacgatgg tctcagccgc gccgctcttc gcggagatcg
     3481 ccccggcctg gctcgaattc gcagaccgcg ccgtcctcgt cgcacacaac gcgcccttcg
     3541 acatccgctt catcaaccac gagctgtcgc tcgtctaccc cggcaggcgg atgtcgaacg
     3601 cgcacatctg cacggtctcg ctcgcgcgcc gcgccgtgcc cgagctgccg agccaccggc
     3661 tccacctgct ggccgaacac ttcgccgtcc acttcccgcg ccgccaccgc gcgggcgacg
     3721 acgcgcgcgc caccgccgag gtcttcatcc gcctcctcga acgcctgcgc cgcgacggcg
     3781 tccacgacct cgccagcgcg cgacagtaca agaggccgga ggctaacggt cggaagtcag
     3841 aggccaaagg tcagaagaca gggaccgtcg gccacaccgt ccgcacgtct ccggtttgat
     3901 agacgacatc tcgaacatga ccggcaggct ttcaacgaag ccgtcgtcat ccctcttcgc
     3961 ttaaactctt gccgcctgct ctctgctctt caccggcccc cggcccccgg cccccggccc
     4021 ctatcttcgt tcttctccgc ttgagcaggt tacgaatcac gaacaccgcg acgatgagga
     4081 cggcgacgcc gaggccgacc gccgggtagt tccgcgccag cacctctttc gcgcgctcgc
     4141 cgtagcgcac cgcgatgaag ccttcgagga ggaagcggaa cgcgcgcccg atgccgaccg
     4201 ccacggcgaa gcgcacgagg ctcaggcgga agacgcccgc cgtgatgacg aacagcttga
     4261 acgggaacgg cggcggcagc accgacgcga ccagcaccga gagcacgtcg tagcggtcaa
     4321 ccagctcctt cacgcgcgcc tgcttcgacg gcgggaagcg ttcgagcgct cggcgccccg
     4381 cccgccgaga gatgcggtag aggatgacgc acccgatgac agacccggcg gtcgccgcca
     4441 gcgcgtagac cggcatccac gccgggcgct gcgtcgagag cagaatcatc accgcgtccg
     4501 gcccgcccgg cagcggaatc aaagccgagt ccaagagcgc gatggcgaac agccccagcg
     4561 gcccgaacgc caccagaaac tgcgacacgc tatccagccc gttcgtgatc ttacgccaca
     4621 gccacttcac ttcgttccgt tctccccttc acgctcctcc gcctcgcgcg cggcgacgct
     4681 ctccgccatg ctgcggcgcg gcgcgacgat gcgctggcgg
//
LOCUS       DBNGOH010001143         1894 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1093739, whole genome shotgun sequence.
ACCESSION   DBNGOH010001143 DBNGOH010000000
VERSION     DBNGOH010001143.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1894)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1894)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1894
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1093739"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="AC159P_22215"
     CDS             <1..322
                     /locus_tag="AC159P_22215"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR3698214.1"
                     /translation="VLAYCVSKAGVDQLTRCAALELAPKGVRVNAVNPGVVVTDIHRR
                     GGMAEENYAAFLERSKQTHPLGRVGEAREVADLVLFLASDRAAWITGATYSIDGGRAQ
                     TCAR"
     gene            426..1670
                     /locus_tag="AC159P_22220"
     CDS             426..1670
                     /locus_tag="AC159P_22220"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR3698215.1"
                     /translation="MLVFYFFAALVLLQSLLSLRGGVRYFAFFRRALEAKRPLYMPFA
                     TVFVPCRGLDQGLRRNLSALFAQHYPAYEIVFVSDQADDPALALADWLASENENPGVV
                     RVRVIEAGRAVESGQKVHNLRAAIAEADPLSEVFVFVDTDARPREGWLRALVAPLEDD
                     LVGAATGYRWFLPVRGGLASHLRSVWNASIASALGENDRGNFCWGGSTAIRRKMFERL
                     GMSERWRGTVSDDYALTSALREERPRIRFVPDCLTASLEDCTFRELLEFTTRQIKITR
                     VYAPHLWRLVLVSNLLFVAVFYGGLALAAARAALGLAWEWPLAVVCLVYAFGTWKAFF
                     RLRAVALVLDDYHAELREGVWAHLLLWPLTAILYLYNALAALFSRRIDWRGITYELKS
                     PTETDIISETVGSGQQAAGSKN"
BASE COUNT          293 a          692 c          568 g          341 t
ORIGIN      
        1 cgtgctggcc tactgcgtct cgaaggcggg cgtcgatcaa ctgacgcgct gcgccgcgct
       61 cgaactcgcg cccaaaggcg tgcgcgtcaa cgccgtcaac cccggcgtcg tcgtcacgga
      121 catccaccgc cgcggcggca tggccgaaga gaattacgcc gccttcctcg aacgctccaa
      181 acagacgcac ccgctcgggc gcgtcggcga ggcgcgcgag gtggccgacc ttgtcctctt
      241 cctcgcctcc gaccgcgccg cgtggatcac cggcgccacc tactccatcg acggcgggcg
      301 cgcgcagacc tgcgcccgat agaaaaatga tgaatgcgga atgttgaatg acgaataaga
      361 cgctgctcgg acgatgacat tcagcttgcc ttccattcat cattccgcat tcatcattgt
      421 ccgtgatgct ggtcttctac ttcttcgccg cgctcgtcct gttgcagagc ctcctctctt
      481 tgcgcggggg cgtgcgctac ttcgccttct tccgccgcgc gttggaggcg aagcgaccgc
      541 tctacatgcc cttcgccacg gtcttcgtgc cgtgccgcgg actcgatcaa gggctgcgcc
      601 ggaatctctc cgcgctcttc gcgcagcact acccggccta cgagatcgtc ttcgtctccg
      661 accaggcgga cgaccccgcg ctcgccctcg ccgactggct ggcgagcgag aacgagaacc
      721 ccggcgtcgt ccgcgtccgt gtaatcgagg cggggcgcgc cgtcgagagc gggcagaagg
      781 tgcacaacct gcgcgcggcc atcgcggagg ccgacccctt gagcgaggtc ttcgtcttcg
      841 tggacacgga cgcgcgcccg cgcgagggct ggctgcgcgc tctggtcgcg ccgctcgaag
      901 acgatctggt cggcgcggcg accggctacc gctggttcct gcccgtgcgc ggcggcctcg
      961 cctcgcacct gcgctcggtg tggaacgctt cgattgcctc ggcgctcggc gagaacgaca
     1021 ggggcaactt ctgctggggc ggctcgaccg ccatccgccg aaagatgttc gagcggctcg
     1081 gcatgagcga gcgctggcgc ggcaccgtct cggacgacta tgcgctgacg agcgcgctgc
     1141 gcgaagagcg cccccgcatc cgcttcgtgc ccgactgcct caccgcctcg ctcgaagact
     1201 gcaccttccg cgagctgctt gagttcacta cgcggcaaat caaaatcacg cgcgtttacg
     1261 cgccgcacct ctggcggctc gtcctcgtct cgaacctgct cttcgtcgcg gtcttctacg
     1321 gcggcctcgc gctcgccgcc gcgcgcgcgg cgctcggcct cgcgtgggag tggccgctcg
     1381 ccgtcgtctg cctcgtctac gccttcggca cgtggaaggc tttcttccgc ctgcgcgccg
     1441 tcgcgctcgt cctcgacgac taccacgccg agctgcgcga aggcgtctgg gcgcacctgc
     1501 tgctctggcc cctcacggcc atcctctatc tctacaacgc gctcgccgcc ctcttctcgc
     1561 gccgcatcga ctggcgcggc atcacctacg aattgaaatc gccgacggaa actgacatca
     1621 tctccgaaac agtaggcagc gggcagcagg cagcaggcag taaaaactaa agaccaagga
     1681 ccaaagacat tcaacgccga cgcgcttttc ttatctgcct gctgcctgct gcccgctgcc
     1741 tacttctgtt atgtccaaca aagccacaca gctctaccga ctgctgcgct cgaccgggcg
     1801 cgagcgaagg ctcggcacgg tcatcttctt cgtcacgtcg cgctgcaacg cgaagtgcga
     1861 gacctgcttc taccacgagg agctgaaccg cccg
//
LOCUS       DBNGOH010001144         2782 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3474893, whole genome shotgun sequence.
ACCESSION   DBNGOH010001144 DBNGOH010000000
VERSION     DBNGOH010001144.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2782)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2782)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2782
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3474893"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            280..1758
                     /locus_tag="AC159P_22225"
     CDS             280..1758
                     /locus_tag="AC159P_22225"
                     /inference="COORDINATES: protein motif:HMM:NF012311.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HIR3698216.1"
                     /translation="MPAANTETPTTAAGHEHSHAQNRFAVIPTPDRLGADTDCAGRGV
                     TIAFLDSGFHPHPDLVEPSDRIKAFFDITGEEPSRGTGRPRPWHWHGTQTTVAAAGNG
                     QLSDGVYRGLAHEAELVLVKVSEGGRITEEAIARGLEWVIENRERYDIRVLNISLGGD
                     EDVPCSRSVIDRAAERAVAAGIVVVVAAGNSGDGERPHSIPPANAPSVITVGGYDDDN
                     LLEPRSLGLYHSNFGPTADGTLKPEIIGPAMWVAAPILPDTDLYRSAECLSRLAAAPD
                     YQLPGLLRSQWREAGLPESVLDGGVGAARGIVESHLRREKIIAAHYQHVDGTSFAAPI
                     VSSVVAQMLEANPKLSPAAVKHILVSTADRVVGAPLARQGSGVVNARRAVAEARREQH
                     ALDGAGYGPPRVCAEGVLFSFHDDAAHEVGVAGDFNGWEASRTPLFKGEGGVWRASVA
                     GLSAGRYRYKFVVDGTRWVDDRANGLKEPDEFGGFNSLLNVV"
     gene            2040..>2782
                     /locus_tag="AC159P_22230"
     CDS             2040..>2782
                     /locus_tag="AC159P_22230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015155608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HIR3698217.1"
                     /translation="MLKALRTHWPEYLIEAAGLGFFMVSACAFGVLLFHPSSPVVAAL
                     PDTVPRRVLMGAAMGATAVAIIYSPWGKRSGAHVNPATTLTFYRLGKVKGWDALFYVA
                     AQFAGGVAGTLMAVMLLGEAVKVPEVNYVVTRGEYGAGVAFAAEVVITFLLMTVVLNA
                     ANSPRLSRWTGLFAGALVLTYISIEQPLSGMSMNPARSFGSAFGARLWAALWVYFTAP
                     LTGMLAAAELYRRTRGRGRVYCAKYHHRN"
BASE COUNT          440 a          959 c          927 g          456 t
ORIGIN      
        1 agctcttcgc gcccgcggga cgcacctcct tgaagacgac gacaaccccg aaggtgtttc
       61 tctccccgcc gaagcccgcg aacgcctgaa gagcgccctc aaccgctcct tccgctagac
      121 cttcaacctt ccgacgccat ctccctgtaa ggatttctct cgccgaacga ccaatcctcg
      181 tagcgcatgg gtgtgcggga atacctcgcg cgtcgccgtg catatagctc tcgcgccgag
      241 ggcagaggct attcggcagg gggaatattt cgagacacga tgccggccgc aaataccgaa
      301 acgccgacca ccgccgcggg acacgagcac agtcacgcgc aaaaccgctt cgccgtcatc
      361 ccgacgccgg acaggctcgg cgccgatacg gactgtgcgg gcaggggcgt caccatcgcc
      421 ttcctcgact ccggtttcca cccgcacccg gacctcgtcg aaccttcgga caggatcaaa
      481 gccttcttcg acatcaccgg cgaggaacca tcgcgcggca cgggtcgacc tcgaccgtgg
      541 cattggcacg ggacgcagac cacggtcgcc gccgccggca acgggcagct ctcggacggc
      601 gtctaccgcg gcctcgcgca cgaggccgaa ctcgtgctgg tgaaggtgag cgagggcggt
      661 cgcatcaccg aggaggcaat cgcgcgcggt ctggagtggg tcatcgaaaa ccgcgagcgt
      721 tatgacatcc gcgttctcaa catctcgctc ggcggcgacg aggacgtacc ctgctcgcgg
      781 agcgtcatcg accgggccgc cgagagggcc gtcgccgcgg ggatcgtggt cgtcgtcgcc
      841 gccgggaact ccggcgacgg cgagaggccg cactccatcc cgcccgccaa cgcgccctcg
      901 gtcatcacgg tcggcggcta cgacgacgac aaccttttgg agcctcggag tctcggtctc
      961 taccattcca acttcggccc gaccgccgac ggcacgctga agcccgagat catcggcccg
     1021 gcgatgtggg tggccgcgcc catcctcccc gacacggacc tctacaggtc tgccgagtgc
     1081 ctgtcccggc tcgccgccgc gcccgactat caactgcccg gcctcctccg ctcgcagtgg
     1141 cgtgaagccg gcctacccga gtcggttctt gacgggggtg tcggcgcggc gcgcgggatt
     1201 gtcgagtcgc acctgcggcg cgagaagatc atcgccgccc actaccagca cgttgacggc
     1261 acgtcgttcg ccgcgcccat cgtctcttcg gtcgtcgcgc agatgctcga ggccaacccg
     1321 aagctctctc ccgccgccgt caagcatatc ctcgtctcga cggctgaccg cgtggtcggc
     1381 gcgccgctcg cgcggcaggg ctccggggtg gtgaacgcgc ggcgcgcggt cgccgaagcc
     1441 cggcgcgagc agcacgcgct cgacggcgcg ggttacgggc cgccgcgtgt ctgcgcggaa
     1501 ggcgtcctct tctcgttcca cgacgacgcg gcgcacgagg tcggggtggc gggggatttc
     1561 aacggatggg aagcttcacg cacgccgctc ttcaaaggcg agggcggagt ctggcgggcg
     1621 agcgtcgccg gtctgtcggc gggccgctat cgctacaagt tcgtcgttga cggcacgcgt
     1681 tgggtggacg accgcgccaa cgggctgaag gagccggacg agttcggcgg cttcaactcc
     1741 ctgctgaacg tcgtttgagg gcgtggcgcc cggaggaagg gttcgagatt acgcggcgta
     1801 agtttttagc gcctgcgacg actaacggat agcgaatgag gttctgccag ccgctcgccg
     1861 tgagcggtgg ggcgtgagga gttgatggcc gtcgtgaagc attcccggat caggaagagg
     1921 gttctgctca tcaggcagag gctcgccgcg cgaaggaagt gggcggcgga ttctcccacg
     1981 acctgcggga ataaaacttt cgccgggttg attcaaaccg atggagacgt tcgagggcga
     2041 tgctgaaagc actccgcaca cactggccgg aatacctgat cgaggccgcc ggtctcggct
     2101 tcttcatggt ctcggcctgc gccttcggcg tcctgctctt ccatccttcg tcgcccgtcg
     2161 tcgcggccct tccggacaca gtcccccggc gcgtgctgat gggggcggcg atgggcgcga
     2221 cagccgtcgc catcatctac tcgccctggg gcaagcgctc cggcgcgcac gtcaaccctg
     2281 cgacgacgct cacgttctac aggctcggca aggtcaaggg ctgggacgca ctcttctacg
     2341 tcgccgcgca gttcgcgggg ggcgtcgccg gaactttgat ggcagtcatg ctgctgggcg
     2401 aggcggtcaa ggtgccggag gtcaactacg tcgtcacgcg aggcgagtac ggcgcgggcg
     2461 tggccttcgc cgccgaagtc gtcatcacgt tcctgctgat gacggtcgtg ctgaacgccg
     2521 cgaacagccc gcgcctctcg cgctggacgg gcctgttcgc gggcgcgctc gtgttgacgt
     2581 acatcagcat cgagcagccc ctttccggga tgagcatgaa cccggcgcgc agcttcgggt
     2641 cggccttcgg cgcgcggctc tgggcggcgc tctgggtcta cttcaccgcg cccctcacgg
     2701 ggatgctcgc cgccgccgaa ctctacaggc gcacgcgcgg gcgggggcgc gtctactgcg
     2761 ccaagtacca ccaccgcaac ga
//
LOCUS       DBNGOH010001145         1581 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_542519, whole genome shotgun sequence.
ACCESSION   DBNGOH010001145 DBNGOH010000000
VERSION     DBNGOH010001145.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1581)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1581)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1581
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_542519"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            40..573
                     /gene="nuoE"
                     /locus_tag="AC159P_22235"
     CDS             40..573
                     /gene="nuoE"
                     /locus_tag="AC159P_22235"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HIR3698218.1"
                     /translation="MSQQFVPNNPLPQGTKVPEEVAVFSPEVEAEIDRHLAKYPVMRS
                     ATLPLMFIVQRERGGWLDPPGVAYLAQRLGVRITDIWEVATFYSMINTEPVGRHHIQI
                     CKTLSCKIMGEPKITEHVCRRLGIKPGETTEDGQFTVSLVECLGSCGTAPMFQIGFDY
                     HENLTPEKVDEILDALP"
     gene            738..>1581
                     /locus_tag="AC159P_22240"
     CDS             738..>1581
                     /locus_tag="AC159P_22240"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045271 - respiratory chain complex I
                     [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="complex I 51 kDa subunit family protein"
                     /protein_id="HIR3698219.1"
                     /translation="MFIGAIGCEPLQLARVHLENSASLDVYRKNGGYEALKRVLDGMA
                     PGDVIEEVKKSALRGRGGAGFPTGMKWSFVPKDSPKPKYVVCNADESEPGTFKDRYLM
                     ERDPHLLIEGMLIAAYALGSKSCYIYTRGEYRYLIDIMDVAIREARDAGLVGDKVLGT
                     DFACEVYTHTGAGAYICGEETALLNSLEGLRGHPRMKPPFPAVAGLYGCPTVVNNVET
                     LTAVPDIIKMGGDAYKALGTEKSGGTKLWSVSGHVNRPGVYELPMGYDDMEKFIMEDC
                     QGIAN"
BASE COUNT          294 a          514 c          511 g          262 t
ORIGIN      
        1 acggttcacg gttcacggtt cacggttcac ggttttctta tgagtcagca gttcgtgccc
       61 aacaacccgc tgccgcaagg tacgaaggtg ccggaggagg tggccgtgtt ctcgcccgaa
      121 gtcgaggccg agatcgaccg ccacctcgcg aagtacccgg tgatgcgctc ggccaccctg
      181 ccgctgatgt tcatcgtgca gcgcgagcgc ggcgggtggc tcgacccgcc gggcgtggcc
      241 tacctcgcgc agcgcctcgg cgtccgcatc acggacatct gggaggtcgc caccttctac
      301 tcgatgatca acaccgagcc ggtcggtcgc caccacatcc agatttgcaa gacgctctcg
      361 tgcaagatca tgggcgagcc gaagatcacc gagcacgtct gccgccgcct cggcatcaag
      421 cccggcgaga cgaccgagga cgggcagttc acggtctcgc tcgtcgagtg cctcggctcg
      481 tgcggcaccg ccccgatgtt tcagatcggc ttcgactacc acgagaacct gacgccggag
      541 aaggttgacg agattctgga cgcgctgcct tgagcctcgg ttaaagagcg gacatgaaca
      601 aagccctcct catcctgctc gccttcggcc tgtgcgcgcc cgccgcggcg cggagcaagt
      661 cgccgccgcg cgcgacgact taaccagccg aaggtcattt ttttggagcc acgaattttc
      721 agagtcgcga agagatcatg ttcattggcg caataggctg cgaaccttta cagctcgccc
      781 gcgttcacct ggagaactcg gcgtcgctcg acgtgtaccg caagaacggc ggctacgagg
      841 ctttgaagcg cgtcctcgac gggatggcgc ccggcgacgt gatcgaagag gtgaagaagt
      901 ccgccctgcg cgggcgcggc ggcgcgggct tcccgacggg catgaagtgg agcttcgtgc
      961 cgaaggactc gccgaagccg aagtacgtcg tctgcaacgc ggacgagtcg gagccgggca
     1021 ccttcaaaga ccgctacctg atggagcgcg acccgcacct gctgatcgaa ggcatgctca
     1081 tcgccgccta cgcgctcggc tcgaagagct gctacatcta cacgcgcggc gagtaccgct
     1141 acctcatcga catcatggac gtggccatcc gcgaggcgcg cgacgcaggg ctggtcggcg
     1201 acaaggtgtt gggcacggac ttcgcctgcg aggtctacac gcacacgggc gcgggcgcct
     1261 acatctgcgg cgaggagacg gcgctgctga actcgctcga aggcttgcgc ggacatcccc
     1321 gtatgaagcc gccgttccct gcggtcgcag gtctctacgg ttgcccgacg gtcgtcaaca
     1381 acgtcgagac gctgaccgcc gtgcccgaca tcatcaagat gggcggcgac gcctacaagg
     1441 cgctcggcac cgagaagagc ggcggcacga agctgtggtc tgtctccggc cacgtcaacc
     1501 gccccggcgt gtacgagctg ccgatgggct acgacgacat ggagaagttc atcatggagg
     1561 actgtcaggg gatcgcgaac g
//
LOCUS       DBNGOH010001146         2589 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2190940, whole genome shotgun sequence.
ACCESSION   DBNGOH010001146 DBNGOH010000000
VERSION     DBNGOH010001146.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2589)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2589)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2589
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2190940"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..872
                     /locus_tag="AC159P_22245"
     CDS             <1..872
                     /locus_tag="AC159P_22245"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF045201.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033922 - peptidoglycan
                     beta-N-acetylmuramidase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="exo-beta-N-acetylmuramidase NamZ family protein"
                     /protein_id="HIR3698220.1"
                     /translation="TRRPKAEHLKQLDALVFDIQDIGARYYTYISTLGYAMEEAAKAR
                     LPVFVLDRPNPINGADVEGPVADPDKLSFISYHTIPVRHGMTVGELAQLFKEQRQIDC
                     DLRVIKMENWRRGMWLDATGQVWVNPSPNMRSLTEATLYPGVGLLETTNLSVGRGTDT
                     PFEVVGAPWLDGRKLAPYLNARGLPGVRFVPVRFTPKASVFKGEECGGVNLIVTDRAR
                     FRPVRTGIELAVALRRLYPNDWKVDDYMRLLVNSDTHGRVKRGDAPEEIERAWQDKLQ
                     EFRRARARALIYR"
     gene            903..1856
                     /locus_tag="AC159P_22250"
     CDS             903..1856
                     /locus_tag="AC159P_22250"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014336.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1/P1 nuclease"
                     /protein_id="HIR3698221.1"
                     /translation="MKKALMPVALLFALLSPQSALAWHDTGHMLVAQIAYLRLTPAAK
                     ERVDKLLVPPQGRRPLIHLCAGYYTPATCEKTYDPVTIAVWMDDYRGDSLNGVYDNWH
                     YINYRPFFDGIPERTDVGPEPVNVLDRINWAMNTLRPMPRQERRDDKRDAEALGFLYH
                     LVGDVHQPLHAATRYTAAKPDGDAGGNGFTIKKPDGTNTSLHFFWDSGAGRFKGFEEP
                     KRPLDQASRERLRALADELMKAHPAESMPEWKSTEPLAWVEESNRIAREFAYTKTKEG
                     EAPSAAYTQEAQRIVGQRVALAGYRMAEVLNRTFVAPLPAP"
BASE COUNT          491 a          885 c          808 g          405 t
ORIGIN      
        1 agacgcgccg gcccaaagcc gagcacctga aacagctcga cgcgctcgtc ttcgacatcc
       61 aggacatcgg cgcgcgctac tacacataca tctcgacgct cggctacgcg atggaggagg
      121 ccgcgaaggc gcgcctcccc gtcttcgtgc tcgaccgtcc gaatccgatc aacggcgccg
      181 acgtggaagg ccccgtcgca gaccccgaca agctctcgtt catctcttac cacacgatcc
      241 ccgtccgcca cgggatgact gtgggcgagc tggcgcagct cttcaaagag cagcggcaga
      301 tcgactgcga cctgcgcgtt attaagatgg agaactggcg gcgcggcatg tggctcgacg
      361 cgacggggca ggtgtgggtc aacccctcgc cgaacatgcg gagcctgacg gaggcgacgc
      421 tctaccccgg cgtcggcctg ctcgaaacga ccaacctctc ggtcgggcgc ggcaccgaca
      481 cgccgttcga ggtcgtcggc gcgccgtggc tcgacgggcg caagctcgcg ccctacctca
      541 acgcgcgggg gctgcccggc gtccgcttcg tccccgtgcg cttcacgccg aaggcttcgg
      601 tcttcaaagg cgaagagtgc ggcggtgtca acctgatcgt caccgaccgc gcgcgctttc
      661 gccccgtccg caccggcatc gagctggccg tcgccctgcg ccgtctctac ccgaacgact
      721 ggaaggttga cgattacatg cgcctactcg tcaactcgga cacgcacggg cgcgtcaagc
      781 gcggcgacgc gcccgaagag atcgagcgcg cctggcagga caagctgcaa gagttccgcc
      841 gcgcccgcgc acgggcgttg atctatcgtt gaagtaaaga caatgacgta ggagatattc
      901 ggatgaagaa agcgttgatg ccggtcgcac tcttgttcgc cctgttgtcg ccgcagagcg
      961 ccctcgcgtg gcacgacacg gggcacatgc tcgtcgcgca gatcgcgtac ctgcgtttga
     1021 cgcccgcggc gaaggagcgc gtcgataagc tgctcgtccc gccgcagggc cgccgcccgc
     1081 tcatccacct ctgcgcaggc tactacacgc ccgccacctg cgagaagact tacgaccccg
     1141 tgaccatcgc cgtctggatg gacgactacc gcggcgactc tttgaacggc gtgtatgaca
     1201 actggcacta catcaactac cgcccgttct tcgacggcat ccccgaacgc acggacgttg
     1261 ggccggagcc tgtgaacgtg ctcgaccgca tcaactgggc gatgaacacg ctgcggccca
     1321 tgccgcggca ggagcggcgc gacgacaagc gcgacgccga agcgctcggc ttcctctacc
     1381 acctcgtcgg cgacgtgcac cagccgctcc acgccgcgac ccgctacacc gccgccaagc
     1441 ccgacggcga cgcgggcggc aacggcttca ccatcaagaa gccggacggc acgaacacca
     1501 gcctccactt cttctgggac tcgggtgccg gtcgcttcaa aggctttgag gaaccgaagc
     1561 gaccgctcga tcaggcttcg cgcgaacgcc tccgcgcgct cgccgacgag ttgatgaagg
     1621 cgcaccccgc agagtcgatg cccgagtgga agagcaccga gccgctcgcg tgggtcgagg
     1681 agagcaaccg catcgcgcgc gagttcgcct acacgaagac gaaggagggc gaggcgccgt
     1741 ccgcggccta cacgcaggag gcgcagcgga tcgtcggcca gcgcgtcgcg ctcgcgggct
     1801 accgcatggc cgaagtcctc aaccgcacct tcgtcgcgcc gctgcccgcg ccttgaaggc
     1861 tctcgcgcga gacgccgcgc cacgcgccga taaagaaccc cggcggagcc gtcgggagcg
     1921 tcggcccggc gcgcgaaggg aaggcgcata caaaaatcgg gacgccgaat ccattttttc
     1981 gtaaccgcgc ggggcgcgcg ccgcgcagcg cgttaggaaa acaggggcga ttggtcgcgg
     2041 ggcgagatgg aacgtatatt gcgtgcttga aatgattcgg cgcgcgcacg aaagcctacc
     2101 gcgtgcgccc cgattcggtt ttgtcgccga acgccggacg agtgacgaaa cggttgtcga
     2161 aggcgctctg tgcgggccga gccaacgcct cgtgaagtat catgaatgct tatttcgata
     2221 ataaattttt ttagcttcac ttatgggacg tttagtcact acaattaggc accgggcatc
     2281 aagcagtcgc gtttccagcc ttcagcagac cgacatgaca ggttaataac gtttcctacc
     2341 ggcgccgctt ccgctagcga agcgcgcacg cacgaacagg ggaatggttc cgaaggggcc
     2401 tcgggtgaca ttcgccggcg caaggcggac tacaggcaca gcccccgtca tcgcccgagc
     2461 cgaaccgctg ttcaacgcta cagccagact accgtgtgag gagctaccca tgaaaactgg
     2521 tcttcccatc cgttccctga tctgcgcgct cgtcgtcacg gtggcgacgg cgatcaccgc
     2581 cttcgggca
//
LOCUS       DBNGOH010001147         5073 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2375116, whole genome shotgun sequence.
ACCESSION   DBNGOH010001147 DBNGOH010000000
VERSION     DBNGOH010001147.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5073)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5073)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5073
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2375116"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..217
                     /locus_tag="AC159P_22255"
     CDS             <1..217
                     /locus_tag="AC159P_22255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3698222.1"
                     /translation="GRAQLAVVGRVTTGIGLACFLMLFASEAGRPRTIITLYAAATTL
                     AGLAFVLVGRTARAARGAEAPAAGAAD"
     gene            286..945
                     /gene="hxpB"
                     /locus_tag="AC159P_22260"
     CDS             286..945
                     /gene="hxpB"
                     /locus_tag="AC159P_22260"
                     /EC_number="3.1.3.22"
                     /EC_number="3.1.3.50"
                     /EC_number="3.1.3.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015229539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HIR3698223.1"
                     /translation="MLSAVIFDMDGVLIDSEPFWQESEIDVFARAGLALTREMCLQTM
                     GLRVDEVVEYWRRRRDFDRTPAGALEDSIIGGVVERILSKGEAREGAAEALEFFRGRG
                     VRVALASSSAYRLIRAVTGRLGLADSFDCIYSAEDEEYGKPHPGVYLTTARRLGVTPT
                     ECLAIEDSFNGVLAAKAARMRCVAVPEPAQRRDPRFRIADLVLDSLAGVDMGLWQRLN
                     D"
     gene            971..2101
                     /locus_tag="AC159P_22265"
     CDS             971..2101
                     /locus_tag="AC159P_22265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018655487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR3698224.1"
                     /translation="MKKVNVALIGYKFMGRAHSNAWRQVATFFEPPVAPELKVVCGRD
                     EAGVRDAAARFGWQEHATDWEQIVRRDDIDLVDICTPGDTHLPIALAAAEAGKVILCE
                     KPLANNVADAERMLDAVRSGGSILLLCHNYRRVPAVALAKRLIEEGRIGEVRHYRGTY
                     LQDWLVNPDVPRVWRLEKSRAGSGALGDLLSHSADLARYLVGEITEVCGMLKTFVPER
                     PAPGGGGVLPVEVDDAALTLVRFEGGAVGSIEATRFATGRRNYNRFEINGSRGSIAFN
                     LERMNELELYTEEGPESGFRTILATDPSHPYMGVWWPPGHIIGYEHTFTHTVLDLLRA
                     VAERRQPKPDFEDGVRTQKILDAVERASLSRTWESVQGAVRQ"
     gene            complement(2394..2978)
                     /locus_tag="AC159P_22270"
     CDS             complement(2394..2978)
                     /locus_tag="AC159P_22270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdE family protein"
                     /protein_id="HIR3698225.1"
                     /translation="MQPLDELLEECGRLHGHMCAGQLLGARMAVLGCRLIGIEDPRGA
                     DRKKLIVWVEIDRCMTDAVSAVTGVRLGKRSLKYLDYGKVAATFYNTETREAVRIAAL
                     EESRELADARHPEIESRKERQFRAYREAADEELFKVERVEVEYGEMDMPGRPRSRVLC
                     ARCGEGVNDGREVAGPEGSVLCRPCASGGYYRRL"
     gene            complement(3264..4079)
                     /locus_tag="AC159P_22275"
     CDS             complement(3264..4079)
                     /locus_tag="AC159P_22275"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit gamma"
                     /protein_id="HIR3698226.1"
                     /translation="MSGSVERFDEEARREVGGWGRTVVRRGELLRHPVYTRFLHWSVA
                     IFFILSLLTGFAIYSPWLYRWLTPLFGGGPMTRFLHPWFGLAFDIAFLFQFLNWFTPM
                     AWTKADSGWLRRIKAYTTNKEKVEAEDVGFFNGGQKLYFRAIAGSAVLFLITGLLLWF
                     DDTVPRWSVAVSYVVHDLAALTMLAGFVIHIYEGTAAQPGTFRSMIDGTVTRAWAWTH
                     HPAWYSDVTGRDPREDYERERRRQVGRERAAEAWEREQDARERAGGAGPRELP"
     gene            complement(4076..5008)
                     /gene="fdxH"
                     /locus_tag="AC159P_22280"
     CDS             complement(4076..5008)
                     /gene="fdxH"
                     /locus_tag="AC159P_22280"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0015944 - formate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit beta"
                     /protein_id="HIR3698227.1"
                     /translation="MSDERTLQIQQISAHPGPVPGRDTQRAVEVCKYIDVTTCFGCKA
                     CEVACVEWNDHPFRETTFDNTYQTMPETDWNYWNLIKFVEHENAGGLMWLMRKDQCMH
                     CEDPGCLRACPADGAVVQYRNGIVDFQQENCIGCGYCISGCPFDIPKFNEKTKKVYKC
                     TLCSDRVGQGLEPACIKACPTGCLHFGTKQDMLALADTRAQQLVQISGFKNAGVYDPQ
                     SIGGTHVIYVLHDATQPEIYGGLPANPTIPPSYTVWKWLAKPTGLLMALMGIFAVFFH
                     RVFYGPKWPQPEPVPVVEVTRPEDEAETKGRQRP"
BASE COUNT          796 a         1727 c         1729 g          821 t
ORIGIN      
        1 gggccgcgcg cagctcgccg tcgtggggcg cgtcaccacg ggcatcgggc tcgcctgctt
       61 cctcatgctg ttcgcatcgg aggccggccg cccccgcacg atcatcacgc tctacgccgc
      121 cgcgacgaca ctggcggggc tggccttcgt cctcgtcggc cgcacggcgc gggcggcgcg
      181 gggcgcggag gcgccggccg ccggggcggc ggactgagct ggcgggcgcc gcggcgcgca
      241 gggcgcttca ccaaccgcgg caagttgata caggtaagtg agccgatgct gagcgcggtc
      301 atcttcgaca tggacggggt gctcatcgac tcggagccct tctggcagga gtccgagatc
      361 gacgtcttcg cgcgggcggg cctcgccctc acgcgcgaga tgtgcctcca gacgatgggg
      421 ctcagggtgg acgaggtcgt cgagtactgg cggcgacggc gcgacttcga ccggacgccc
      481 gccggcgcgt tggaagactc gatcatcggc ggggtcgtgg agaggatttt gtcgaagggc
      541 gaggctaggg agggcgcggc ggaggcgctg gagttcttca gggggagggg cgtgcgcgtc
      601 gcgctcgcct cctcgtcggc ctaccggctc atccgcgccg tcaccgggcg gctcggcctc
      661 gcggacagct tcgactgcat ctactccgcc gaggacgagg agtacggcaa gccgcacccc
      721 ggcgtctacc tgaccaccgc gcggaggctc ggcgtcacgc cgaccgagtg cctcgcgata
      781 gaagattctt ttaacggcgt gctcgcggcc aaggcggcgc ggatgaggtg cgtcgccgtc
      841 cccgagccgg cacagcggcg cgacccgcgc ttccgtatcg ccgacctcgt gctcgactcg
      901 ctcgcgggcg tggacatggg actgtggcag cgcctcaacg actgatgcgg cgggcgcatc
      961 gcgaatcggc atgaagaaag tcaacgtcgc actcatcggc tacaagttca tggggcgcgc
     1021 gcacagcaac gcctggcggc aggtggccac gttcttcgag ccgccggtcg cgccggagct
     1081 gaaggtcgtc tgcgggcgcg acgaggccgg cgtccgggac gcggcggcgc gcttcggctg
     1141 gcaggagcac gcgaccgact gggagcagat cgtgcgccgc gacgacatcg acctcgtgga
     1201 catctgcacg ccgggcgaca cgcacctgcc catcgcgctc gccgccgccg aggccggcaa
     1261 ggtcatcctc tgcgagaagc cgctcgccaa caacgtcgcc gacgccgagc ggatgctcga
     1321 cgccgtccgc tcgggcggct ccatcctctt gctctgccac aactaccggc gcgtcccggc
     1381 ggtcgcgctc gccaagcggc tcatcgagga ggggcgcatc ggcgaggtgc gacactaccg
     1441 cggcacgtat ttacaggact ggctcgtcaa cccggacgtg ccgcgcgtct ggcgtttgga
     1501 gaagtccagg gccggctcgg gcgcgctcgg cgacctcctc tcgcactccg ccgacctcgc
     1561 gcggtatctg gtcggcgaga tcacggaggt ctgcggcatg ctcaagacct tcgtcccgga
     1621 gcgccccgcc cccggcggcg gcggcgtgct gcccgtggag gtggacgacg cggcgctcac
     1681 gctcgtccgc ttcgaagggg gagccgtcgg ctccatcgag gcgacgcgct tcgccaccgg
     1741 gcgccggaac tacaaccgct tcgagatcaa cgggagtcga ggcagcatcg ccttcaacct
     1801 tgagcggatg aacgaactcg aactctacac cgaggagggg ccggagagcg gcttcaggac
     1861 catccttgcg accgacccgt cgcacccgta catgggggtc tggtggccgc ccggccacat
     1921 catcggctac gaacacacct tcacgcacac cgtgctcgac ctgctgcgcg ccgtcgccga
     1981 acggcggcag ccgaagccgg acttcgagga cggcgtgcgg acgcagaaga ttctggacgc
     2041 cgtcgagcgc gcttcgcttt cgcgcacgtg ggagagcgtc cagggggctg tgcgccaatg
     2101 accgcctccg cccgctggcg tgtcaggcga tgagacggct cggggccggc ttcacgtgtg
     2161 ggcgcgctca ctgcgcagtc gctacggcgg cctccccgtc gccgtctgcc tggagcaggc
     2221 gcgcggcccc ctcatccacg ccctcctgaa gtacgaccac ctgagagcac gtacaggaag
     2281 atgggggacg cggcgcgcgg gacggacgag tccggaatcg cctcgtaagc gtaagtcgtc
     2341 ggcatcacac aatccctccc gtggcagacc gagagcgtac gtgccgcgcc ctttcagagc
     2401 ctccggtagt acccgcccga cgcgcagggg cgacacagca cggagccttc ggggcctgcc
     2461 acctcgcgcc cgtcgttgac gccctcgccg caccgcgcgc aaaggacgcg cgaccgaggc
     2521 cggcccggca tgtccatctc gccgtactcg acctcgaccc gctcgacttt gaatagctct
     2581 tcgtcagcgg cctcgcggta ggcgcggaac tgccgctcct tcctgctctc gatttcgggg
     2641 tgacgcgcgt cggccaactc gcgcgactct tcgagcgccg cgatgcgcac cgcctcgcgc
     2701 gtctccgtgt tgtagaacgt cgccgcgacc ttgccgtaat ccaggtactt caaactgcgc
     2761 ttgccgaggc gtacgcccgt cacagccgag acggcgtccg tcatgcagcg gtcgatctcc
     2821 acccacacga tcagcttttt acggtcggcg ccgcgcgggt cttcaatgcc gatcaggcgg
     2881 cagccgagca cggccatccg tgcgccgagc aactggccgg cgcacatgtg gccgtgcagg
     2941 cggccacact cctccaagag ttcgtcaagc ggctgcatgt cctactcccc tgcttcagaa
     3001 agtaaagatc gaagcgaact ccgagcgcac gttatgcgtc gcgtagctcg ggatgccgcg
     3061 gaacagcggc agcgcccccg cgcggttggc cgcgcgcgtg cggtagtagc cttgctcgta
     3121 ggcgaaggtg acgaatcata atgtcagcct cacttttgtc gaaatcgccg ggcgggcagg
     3181 ggcaaactcg aatgaggggc gacggggcgg ttgaggtgtg gccttttatc tcagccccgt
     3241 gcggcgggcc ggatgagtta acgctacggc agctctctcg ggccggctcc gccggcacgc
     3301 tcgcgcgcgt cctgctcgcg ctcccacgcc tctgcggcgc gctcgcgccc gacctgccgg
     3361 cggcgctcgc gctcgtagtc ctcacgcgga tcgcgccccg tgacatcact gtaccacgcc
     3421 gggtgatgag tccatgccca cgcccgcgtc accgtgccgt cgatcatcga gcggaaggtg
     3481 ccgggctggg ctgccgtccc ttcgtagatg tggatgacga atcctgcgag catggtgagc
     3541 gccgcaaggt cgtggacgac gtagctgacg gcgaccgacc agcgcggcac ggtgtcgtcg
     3601 aaccacagca gcagtccggt aatcagaaag agcacggcgc tcccggcgat ggcccggaag
     3661 tagagtttct ggccgccgtt gaagaagccc acgtcctcgg cttcgacctt ctccttgttc
     3721 gtcgtgtacg ccttgatgcg gcggagccat ccgctgtctg ccttagtcca cgccatcggg
     3781 gtgaaccagt tgaggaactg gaagaggaag gcgatgtcga acgcgaggcc gaaccacggg
     3841 tggaggaagc gcgtcatcgg gccgccgccg aagagcggcg tgagccagcg gtagagccac
     3901 ggcgagtaaa tggcgaagcc ggtgagcaga gagaggatga agaagatcgc caccgaccag
     3961 tggagaaagc gcgtgtagac ggggtggcgc agcagttcgc cgcggcggac gaccgtgcgc
     4021 ccccacccgc cgacctcgcg ccgcgcctcc tcgtcgaagc gttcgaccga gccgctcatg
     4081 gcctctgcct ccccttcgtc tccgcctcgt cttcgggccg cgtcacttcc accacgggga
     4141 cgggttcggg ctgcggccat ttaggcccgt agaagacgcg gtggaagaag accgcgaaga
     4201 tgcccatcaa cgccatcaac aaccccgtcg gcttggcgag ccacttccag acggtgtagc
     4261 tcggcgggat ggtcgggttc gcgggcagcc cgccgtagat ttccggctgc gtcgcgtcgt
     4321 gcagcacgta gatgacgtgc gtgccgccga tcgactgcgg gtcgtagacg cccgcgttct
     4381 tgaagcccga aatctgcacc aactgctgtg cgcgcgtgtc ggccagggcc agcatgtctt
     4441 gcttcgtgcc gaagtggaga cacccggtcg ggcacgcctt gatgcaggcg ggctcaagcc
     4501 cctgcccgac gcggtcggag cagagcgtgc atttatagac cttcttcgtc ttctcgttga
     4561 acttcgggat gtcgaacggg cagcccgaga tgcagtagcc gcagccgatg cagttctcct
     4621 gctggaagtc cacgatgccg ttccggtact ggacgaccgc gccgtccgcg gggcacgcgc
     4681 gcaggcagcc ggggtcttca cagtgcatgc actgatcctt gcgcatcagc cacatcagcc
     4741 cgcccgcgtt ctcgtgctcg acgaacttga tgaggttcca gtagttccag tcggtctccg
     4801 gcatcgtctg gtaggtgttg tcgaaggtcg tctcgcggaa cgggtggtcg ttccactcga
     4861 cgcaggccac ctcgcacgcc ttgcagccga agcaggtcgt cacgtcgatg tacttgcaga
     4921 cttcgaccgc gcgctgcgtg tccctgccgg ggacggggcc ggggtgggcc gagatttgtt
     4981 ggatttgcag agtcctctcg tcgctcatgc tctctccatt gcggaatgcg gatttcggat
     5041 tgcggattcg aaaaccgcga cccgaacttt cac
//
LOCUS       DBNGOH010001148         5616 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2191233, whole genome shotgun sequence.
ACCESSION   DBNGOH010001148 DBNGOH010000000
VERSION     DBNGOH010001148.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5616)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5616)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5616
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2191233"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(193..1014)
                     /locus_tag="AC159P_22285"
     CDS             complement(193..1014)
                     /locus_tag="AC159P_22285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3698228.1"
                     /translation="MASNCKLIRAEIEETAGGAWPSRGTEAHLAACANCLSFYEEREA
                     LRRLVGDLERVEAPGDFEFRLRARMAAAKETEGTRPFVLRFAPGFASVALAAVFVAAV
                     AGSYYFKRTAPAVADHTASRTSSNDVTQTAASHPTPTAAAPTEGLAVEVSKQVNEQAG
                     GSSPLDVGRAVGVRQSKPERGHGARAAAAEARREVAAGRTEGELSFGLSTSPVIKASN
                     VKLEVSAEPLRMVVRDESGAARRVAMKPVSFGAQNPVGKPRAVSNRSLADDEGVW"
     gene            complement(1033..1701)
                     /locus_tag="AC159P_22290"
     CDS             complement(1033..1701)
                     /locus_tag="AC159P_22290"
                     /inference="COORDINATES: protein motif:HMM:NF019265.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR3698229.1"
                     /translation="MSAVSTTNELVFSAAAAGPGAVSAAPRVAEAQSDHGLLAATLTG
                     DEAAFGELVRRYRNQITNYIYRMTNDYDTAVDLAQETFMRVYAAAERYQTSYAFSTYI
                     YRIATNLAISELRRRKRRRLVSLTGFFQSRDTSQAEAAELDVPDARPLQDATYVEDER
                     RSAVARAISTLPEKYRAPLVLRDVEERSYEEIAAILQMSEGTVKSRINRARTFLRDKL
                     RAHL"
     gene            2043..4517
                     /locus_tag="AC159P_22295"
     CDS             2043..4517
                     /locus_tag="AC159P_22295"
                     /inference="COORDINATES: protein motif:HMM:NF012257.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HIR3698230.1"
                     /translation="MPTEITKHQTILDAIAKTDIISELLDKHHGHYEHELDIEVIAYG
                     RNYNGKKVGPYVHLYEYKGGEEIIREGDWGGNTFYVAVDGTLDVYVRDKKTSENNKVA
                     ELPPGTSFGEMSILAGVPRNATIVVPPDTKATVLEVERPALRLLRKLPKFGEQLDQTY
                     RKHGLGRTLVDVREVTGQAFSPELIDKLGKSARFMVYAKHHALFNEGEPVDRIIFIKS
                     GWVRRVRGLGYEAALGDLVVSGLTEDVGVDFLGAGNCLGLEGLPRDIDWGYTATVLQR
                     TEVLEISIPRLRADPALRAALERSFSEFSPADDDPHPASVQNKPAVAATEREIATGVV
                     DGTNLLVMDMDKCVRCGNCSMACHQVHGHSRLLRRGINIERPANFGGNSANHVLVPSV
                     CMHCQDPECLTGCPTGAIGRFQNGQIDINPKTCIGCGDCATQCPYNAITMIPRRPETP
                     KPATLVQRTKAWLSLAPFTPPQPVTAVDNLLATKCNLCNGTTLNPKGAKGQAYSCEEN
                     CPTGALVRVNPREYFSEVNERLGLIYRDQTHAIGRNIHKRDPKALAWHAFGILATLAV
                     TAATVWALFRYGIDERLGGTWLTVRWITGLVGLGGIAFVMTYPFRKQVYRRRAGALRY
                     WMLSHVYLGLVAGVVLLLHGGPSTGGLLTSLLMISFDAVILTGLFGIAVYLIAPRIMT
                     SIEVEPLLVEDLRARSDELRETLTDIGLKSNESLREFIRKEVRGKVFSFSYLVRQYLR
                     REPLSSLLAAAREEYEAKASSLDPESRRLLLEAVETTVTLRRVDSLIYLHQLLKLWLA
                     PHVVTTALMLVLMVVHIIQVVFFLVR"
     gene            4705..>5616
                     /locus_tag="AC159P_22300"
     CDS             4705..>5616
                     /locus_tag="AC159P_22300"
                     /inference="COORDINATES: protein motif:HMM:NF012708.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HIR3698231.1"
                     /translation="MQTQASKFVITREDLNVDPLTLVTEGLKIGRLPSSEIVLNHPTV
                     SRLHAGINEAEGRFYLFNFSHSSGTTLNGRVVAVEEAEVLADGDVIQIGPFFLYIDRD
                     DEALSIRVALQMAVNIGDAEVRQDAQSPGEDKKPEEEPSEEVSQALNVFWEKRKREAG
                     KMQRLSPMRPHAPSRVLGKARFNWTPTRDLVRPWPFSVFIWALVIVGALSVVAAYAYA
                     DAFSPAPISNAHARSSFQSQPAIAKQPNAASCTTCHSLTGTMDGNCTSCHTTPAFTST
                     VTKEHHAAGIGCTACHVEHQGTEYRPQV"
BASE COUNT          938 a         2056 c         1743 g          879 t
ORIGIN      
        1 cggtggcgcc gccgccgccg tcgcctcctg cgcgcgcgcg attgccgccc ctccgaaaca
       61 gagccagacg ccacacgcga ccgacgcaca atgtccgagt ctcttaagca tttcccctga
      121 aaccgaaaga ccgctccgcc accgactaca agaccgccga ccgtcaagcc cgccgaccgg
      181 cgaggccgcc cattaccaga cgccttcgtc gtcggcgagc gacctgttcg agacggcgcg
      241 cggtttgccg accgggttct gcgcgccgaa gctcacgggc ttcatcgcca cgcggcgggc
      301 cgcgccgctc tcgtcgcgca cgaccatgcg cagaggctcc gccgagactt cgagcttcac
      361 gttcgaggct ttgatgaccg gcgacgtgga gaggccgaaa ctcagctcgc cctccgttcg
      421 ccccgccgcc acttccctgc gcgcctccgc cgcagccgca cgcgcgccgt gcccgcgctc
      481 cggcttcgac tgacgaacgc caacggctct cccgacatct agcggcgacg aaccgcccgc
      541 ctgctcgttc acctgtttcg agacttcgac cgcaagcccc tcggtcgggg ccgccgccgt
      601 gggcgtcggg tgactggcgg ctgtctgcgt cacgtcgttt gacgacgttc gagaggccgt
      661 gtggtctgcg accgccgggg ccgtgcgctt gaagtagtaa gaccccgcga ccgccgcgac
      721 gaagacggcg gcgagcgcga cggaggcgaa gccgggcgcg aaacggagga cgaaaggtcg
      781 cgtgccctcc gtctccttcg cggcggccat gcgcgcgcgc aggcggaact cgaaatcgcc
      841 gggcgcctcg acccgttcga gatcgccgac gagacgccgc agcgcctcgc gctcttcgta
      901 gaacgagagg cagttcgcgc acgccgcgag gtgcgcctcc gtaccgcggc tcggccacgc
      961 gccgcccgcc gtctcttcga tctccgccct gatgagtttg cagttacttg ccatgatcct
     1021 tgcttccccg tcttacaagt gcgcccgcag cttgtcgcgc aggaacgtgc gcgcgcggtt
     1081 gatgcgcgac ttcacggtgc cctcgctcat ctgcaagatg gccgcgatct cttcgtagct
     1141 ccgctcctcc acgtcgcgca gcacgagcgg cgcgcggtac ttctcgggca gcgtcgagat
     1201 ggcgcgcgcg accgccgacc gccgctcgtc ctcgacgtac gtcgcgtcct gcaaaggccg
     1261 cgcgtccggc acgtccagct ccgccgcctc ggcctgagag gtgtcgcggc tctggaagaa
     1321 gccggtcaaa gagacgagcc gacggcgctt gcgccgacgc agctcgctga tggcgaggtt
     1381 cgtggcgatg cggtagatgt aggtcgaaaa agcgtagctc gtctggtagc gctccgccgc
     1441 cgcgtagacg cgcatgaagg tttcctgcgc cagatcgacc gccgtgtcgt agtcgttcgt
     1501 catgcggtag atgtagttcg tgatctggtt gcggtagcga cggaccagct cgccgaacgc
     1561 cgcctcgtcg cccgtcagcg tcgccgccag caggccgtgg tcgctctggg cctcggcgac
     1621 gcgcggcgcc gcggacacgg cgcccggccc ggcggctgcc gccgagaaga caagttcgtt
     1681 cgtcgtggag actgcgctca tcgagggaag gtgggccgcc cgaaatccga gtccgccgaa
     1741 acccgataaa actcgccgga gattccgccc gccgaagggg gccgcgcggg taggattata
     1801 caccccgacc gcgagcgccc cgcagggaat acacgccgcc cccgccggtc cggttcccta
     1861 attttttcgg cgggaagaaa aatcttgcca cccacatgca aaacgggtaa tttgcacggc
     1921 gcggcgagat gctataatgc gcccgctcaa ttctaaagtt tcttcattcg gcccggcggc
     1981 tctctcgccc ggcgcttcgg ctccctttga aatctgccgc tccgagctta agacacgagg
     2041 ccatgcccac agagatcacg aagcaccaga ccattctcga cgccatcgcg aagaccgaca
     2101 tcatctccga gctgctcgac aaacaccacg gccactacga gcacgaactc gacatcgagg
     2161 tcatcgccta cgggcgcaac tacaacggca agaaggtcgg cccctacgtc cacctctacg
     2221 agtacaaggg cggcgaggag atcatccgcg agggcgactg gggcggcaac accttctacg
     2281 tcgccgtcga tggcacgctc gacgtgtacg tccgcgacaa gaagacctcg gagaacaaca
     2341 aggtggcgga gctgccgccg ggcacgagct tcggcgagat gtccattctc gcgggcgtgc
     2401 cgcgcaacgc gaccatcgtg gtgccgcccg acacgaaggc gaccgtcctc gaagtcgagc
     2461 gcccggcgct gcgcctgctg cgcaagctcc cgaagttcgg cgagcagctc gaccagacct
     2521 accgcaagca cggcctgggc cggacgctcg tggacgtgcg cgaggtcacg ggccaggcgt
     2581 tcagcccgga gctgatcgac aagctcggca agtccgcgcg cttcatggtc tacgccaagc
     2641 accacgccct cttcaacgag ggcgagccgg tggatcgcat catcttcatc aagagcggct
     2701 gggtgcggcg cgtgcgcggc ctcggctacg aggcggcgct cggcgacctc gtcgtctccg
     2761 gcctcacgga ggacgtgggc gtggacttcc tcggcgcggg caactgcctc ggcctcgaag
     2821 gtctcccgcg ggacatcgac tggggctaca cggcgaccgt gctccagcgc accgaggtgc
     2881 tggaaatctc gatcccgcgt ctgcgcgcag accccgccct gcgcgcggcg ctggagcgct
     2941 ccttctccga gttctcgccc gccgacgacg acccgcaccc cgcctccgtc cagaacaagc
     3001 cggcggtcgc cgccaccgag cgcgagatcg ccaccggcgt cgtggacggc acgaacctgc
     3061 tcgtgatgga catggacaag tgcgtgcgct gcggcaactg ctcgatggcc tgccatcagg
     3121 tgcacggcca ctcgcggctg ctgcgccgcg gcatcaatat cgagcgcccg gcgaacttcg
     3181 gcggcaactc cgccaaccac gtcctcgtcc cctcggtctg catgcactgc caggacccgg
     3241 agtgtttgac gggctgcccg accggggcca tcggtcgctt ccagaacggg cagatcgaca
     3301 tcaacccgaa gacctgcatc ggctgcggcg actgcgcgac gcagtgcccc tacaacgcca
     3361 tcacgatgat cccgcgccgg cccgagacgc cgaagcccgc caccctcgtg cagcgcacga
     3421 aggcgtggct cagcctcgcg ccgttcacgc cgccgcagcc cgtgaccgcc gtcgataacc
     3481 tgctcgccac caagtgcaac ctctgcaacg gcacgacgct caacccgaag ggcgcgaagg
     3541 ggcaggccta ctcgtgcgag gagaactgcc cgacgggcgc gctcgtgcgc gtcaacccgc
     3601 gcgaatattt ctccgaggtc aacgagcggc tcggcctcat ctaccgcgac cagacgcacg
     3661 ccatcggtcg caacatccac aagcgcgacc cgaaggcgct cgcgtggcac gccttcggca
     3721 tcctcgccac gctcgccgtc acggcggcca ccgtctgggc gctcttccgc tacggcatcg
     3781 acgagcggct cggcggcacg tggctcacgg tgcgctggat cacgggcctc gtcggcctcg
     3841 gcggcatcgc gttcgtgatg acctacccct tccgcaagca ggtctaccgg cggcgcgcgg
     3901 gcgccctgcg ctactggatg ctctcgcacg tctacctcgg ccttgtcgca ggcgtcgtgc
     3961 tgctgctcca cggcggcccc tcgacgggag ggctgctgac ctcgctcctg atgatctcgt
     4021 tcgacgccgt catcctgacg ggcctgttcg gcatcgcggt ctacctcatc gccccgcgca
     4081 tcatgacgag catcgaggtc gagccgctgc tggtcgaaga cctgcgcgcg cggagcgacg
     4141 agctgcgcga gacgctgacc gacatcggcc tcaagagcaa cgagtctttg cgcgagttca
     4201 tccgcaagga ggtgcgcggc aaggtcttct ccttctcgta cctcgtgcgc cagtacctcc
     4261 ggcgcgagcc gctgtcgagc ctgctcgcgg cggcgcgcga agagtacgag gcgaaggcga
     4321 gcagcctcga ccccgagtcg cgcaggctcc tgctcgaagc cgtcgagacc acggtcacgc
     4381 tgcggcgcgt ggactcgctc atctacctgc accaactcct caagctctgg ctcgcgccgc
     4441 acgtcgtcac caccgccctg atgctcgtgc tgatggtcgt ccacatcatt caggtcgtct
     4501 tcttcctcgt gcgctgaacc ggatatgaga aggcaaaagg caaaaggcga aaggcaagag
     4561 gcaaaagggc ggacaaagct ccgctttgat tccatgaatt tccatctcac gccgcaggcg
     4621 tgccggcact tctgcctctt gcctttcgcc tcttgccttt tgcctttcat ccctaccctt
     4681 tgcccttcgg cttccgccta acgtatgcag actcaggcca gcaaattcgt catcacccgc
     4741 gaagacttga acgtcgatcc gctgacgctc gtcacggagg ggctgaagat cgggcggctg
     4801 ccgagcagcg agatcgtgct caaccacccg acggtctcgc gcctccacgc cggcatcaac
     4861 gaggccgagg ggcgcttcta cctcttcaac ttcagccatt cgagcgggac gacgctgaac
     4921 ggtcgcgtcg tcgccgtcga ggaggccgaa gtcctcgccg acggcgacgt gatccagatc
     4981 ggccccttct tcctctacat cgaccgcgat gacgaggcgc tgagcatccg cgtcgcgctc
     5041 cagatggccg tcaacatcgg cgacgccgag gtgcgtcaag acgcgcagtc gccgggcgag
     5101 gacaagaagc cggaggagga gccgagcgag gaggtctcgc aggcgctcaa cgtcttctgg
     5161 gagaagcgca agcgcgaggc cggcaagatg cagcgcctct cgccgatgcg accgcacgcg
     5221 ccctcgcgcg tcctcggcaa ggcgcgcttc aactggacgc cgacgcgcga cctcgtccgc
     5281 ccgtggccct tctcggtctt catctgggcg ctcgtcatcg tcggcgcgct ctccgtggtc
     5341 gccgcctacg cctacgccga cgccttctcg cccgcgccca tctccaacgc gcacgcgcgc
     5401 agcagctttc agtcacagcc cgcgattgcc aaacagccga acgccgcctc gtgcacgacc
     5461 tgtcattcgc tgaccggcac gatggacggc aactgcacgt cctgccacac gacccccgcc
     5521 ttcacctcga ccgtgaccaa agagcaccac gccgccggca tcggctgcac cgcctgccac
     5581 gtcgagcacc agggcacgga gtaccgcccg caggtc
//
LOCUS       DBNGOH010001149         2826 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_725093, whole genome shotgun sequence.
ACCESSION   DBNGOH010001149 DBNGOH010000000
VERSION     DBNGOH010001149.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2826)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2826)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2826
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_725093"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            194..1915
                     /locus_tag="AC159P_22305"
     CDS             194..1915
                     /locus_tag="AC159P_22305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HIR3698232.1"
                     /translation="MLKSPSRVFLSAVLLAPLLAFAGCAAPTQPTAPNPLAELKPALD
                     SVNADDLMRHTKTLASDEYEGRSPGTRGEELSVEYLTGEFQRMGLKPGNPDGTYVQKV
                     PLAGLKAEPRLTLSAGGRQTELKYPDDYVAASRRFVPETRVENSDIVFVGYGVVAPEY
                     GWDDYKGVDVRGKTIVMLINDPAVPSPTNPAELDEKTFKGKAMTYYGRWTYKYEIASE
                     KGAAAAIIVHEDGPAGYPYEVVKGSWAQENFDIQTPDKNMGRVGVESWIHLDRAKELF
                     SSAGQDFDALKKAAASKDFKPVALDAKASFAVRNTLREVQSHNVVARLEGSDANLKNE
                     YVVYTAHWDHLGQNDALQGDRIYNGAVDNASGAAQLLELAEAFTKLPVPPKRSVLFLA
                     VTAEEKGLLGAKFYATNPLYPLARTLANINMDMANQWGRTRDITIVGDGNSTLDDLAR
                     EVAAMQGRAVSPDPEPEKGFYYRSDHFEFAKQGVPALYPDTGTEYVGKPPEYGKQKRD
                     EYTNIDYHKPTDEVKPDWDLSGAVEDAQFMFIVGHRVAAGDKYPEWKPGTEFKAKRDE
                     MLKNAPR"
     gene            2035..2751
                     /locus_tag="AC159P_22310"
     CDS             2035..2751
                     /locus_tag="AC159P_22310"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIR3698233.1"
                     /translation="MNKTDVHNVIRAALFLLCIACPCAARQTTPQTEQTATPAKKANA
                     RPEASAAPTPEPFDGASVAKMGAQCVTLDTEAGAVEVEMLAEAAPETVRNFLNLAATG
                     AFDTTNFSRVVRGFVVQGGNLSTRETMTAELARRARRTLPDEPNAVKHTRGILSMARG
                     EEPNSASTHFFILVGEGPHLDGTFAAFGRVRAGMDVVDKINAAPLDGEKPEKPVRLRR
                     ASVAPCPAVPATPPPAESKP"
BASE COUNT          543 a          967 c          929 g          387 t
ORIGIN      
        1 gaggcgccgc cgcaacgttt gtgcggaggg gcgccgcgcg ggttgtccac gggcgcgacg
       61 tacacgcaaa gcggaacgag gcggcgggcg ggcgccggcg atggattcag cggttgactt
      121 ggcgcgccct ttgacattag aaatcagttg ttcgctctcc tgacatcccg cgaaaagtga
      181 ggcaccgacc gcgatgctga aaagtccgtc ccgcgtgttt ctgtccgccg tcctgctcgc
      241 ccctctgctc gccttcgccg ggtgtgccgc gccgacgcag ccgaccgccc cgaacccgct
      301 cgccgagctg aagcccgcgc tcgacagcgt gaacgcggac gacctgatgc gccacacgaa
      361 gacgctcgcg tcggacgagt acgaggggcg cagccccggc acgcgcggcg aagagctgtc
      421 ggtcgaatac ctgaccggcg agttccagcg catgggcctc aagcccggca accccgacgg
      481 cacctacgtc cagaaagtcc cgctcgccgg cctgaaggcc gagccgcgtc tcaccttgag
      541 cgcgggcggc aggcagacgg agctgaaata cccggacgac tacgtcgccg cctcgcgccg
      601 cttcgtgccc gagacgcgcg tcgagaactc cgacatcgtc ttcgtcggct acggcgtggt
      661 cgcgcccgag tacggctggg acgactacaa gggcgtggac gtgcgcggca agaccatcgt
      721 catgctcatc aacgacccgg cggtgccgag tccgacgaac cctgcggagc ttgacgagaa
      781 gacgttcaag ggcaaggcga tgacctacta cgggcgctgg acgtacaagt acgagatcgc
      841 ctcggagaag ggcgccgccg ccgccatcat cgtccacgag gacgggcctg ccggctaccc
      901 ttacgaggtg gtcaagggca gttgggcgca ggagaacttc gacatccaga cgccggacaa
      961 gaacatgggg cgcgtcggcg tcgagtcgtg gatacacctc gaccgcgcga aggagttgtt
     1021 cagctcggcg gggcaggact tcgacgcgct gaagaaggcc gccgcgtcga aggatttcaa
     1081 gccggtcgcg ctcgacgcga aggcttcgtt cgcggtcagg aacacgctgc gcgaggtgca
     1141 gtcgcacaac gtcgtcgccc ggctcgaagg ctcggacgcg aacctcaaga acgagtacgt
     1201 cgtctacacc gcgcactggg atcacctcgg ccagaacgac gcgctccagg gcgaccggat
     1261 ttacaacggc gcggtagaca acgcctccgg cgcggcgcaa ctgctcgaac tcgccgaggc
     1321 gttcacgaag ctgcccgtcc cgcccaagcg ctccgtcctc ttcctcgccg tgacggctga
     1381 agagaagggg ctgctcggcg cgaagttcta cgcgaccaac ccgctctacc cgctcgcgcg
     1441 cacgctcgcc aacatcaaca tggacatggc caaccagtgg ggccgcacgc gcgacatcac
     1501 catcgtcggc gacggcaact cgacgctcga cgatctggcg cgcgaggtgg ccgcgatgca
     1561 gggccgcgcc gtcagccccg accccgagcc tgagaaaggc ttctactacc gctccgacca
     1621 cttcgagttc gccaagcagg gcgtgcccgc gctctacccc gacaccggca cggagtacgt
     1681 cggcaagccg cccgagtacg gcaagcagaa gcgcgacgag tacacgaaca tcgactacca
     1741 caagccgacg gacgaggtga aaccggactg ggacttgtcg ggcgcggtcg aggatgcgca
     1801 gttcatgttc atcgtcggcc accgcgtcgc cgcgggggac aagtaccccg agtggaagcc
     1861 cggcacagag ttcaaagcca agcgcgacga gatgttgaag aacgcgccgc ggtgaaatgc
     1921 agcaggcagc aggcagcagg cagttagttg agcgaggacg gcgttgacgc ttgcaccctg
     1981 acctttcgat agagaaagtt ttactgccta ctgcctactg ctttaatcaa gccgatgaat
     2041 aaaacggacg tgcataacgt gattcgggcc gcgctgtttc tactctgcat cgcgtgcccg
     2101 tgcgccgcgc ggcagacgac tccgcagacg gagcagacgg cgacgcccgc caagaaagcg
     2161 aacgcgcgtc ccgaagcctc tgcggcgccg acgccggagc ctttcgacgg cgcgagcgtg
     2221 gcgaagatgg gcgcgcagtg cgtcacgctc gacacggagg cgggcgccgt cgaggtcgag
     2281 atgctcgccg aagccgcgcc cgagaccgtg cgcaacttcc tcaacctcgc cgccaccggc
     2341 gccttcgaca cgaccaactt cagccgcgtc gtccgcggct tcgtcgtgca gggcggcaac
     2401 ctctcgacgc gcgagacgat gacggcggag ctggcccggc gcgcgcgccg cacgcttccg
     2461 gacgagccga acgccgtcaa gcacacgcgc ggcatcctct cgatggcgcg cggcgaagag
     2521 ccgaacagcg cgagcacgca cttcttcatc ctcgtcggcg aaggcccgca cctcgacggc
     2581 acgttcgcgg ccttcggtcg cgtccgcgcg gggatggacg tggtggacaa gatcaacgcc
     2641 gcgccgctcg acggcgagaa gccagaaaaa cccgtccgcc tccggcgcgc ctcggtcgcg
     2701 ccctgccccg ccgtccccgc gacgccgccg cccgccgaat cgaagcccta gagcaatttg
     2761 cggttgagtt taccaacggc aggatttaac gcagagttcg cggaggcgca gagggcgcag
     2821 agaaaa
//
LOCUS       DBNGOH010001150         2715 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_177294, whole genome shotgun sequence.
ACCESSION   DBNGOH010001150 DBNGOH010000000
VERSION     DBNGOH010001150.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2715)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2715)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2715
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_177294"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2109
                     /locus_tag="AC159P_22315"
     CDS             <1..2109
                     /locus_tag="AC159P_22315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HIR3698234.1"
                     /translation="ELKEGDRLLVGNSVLFSPGRIDNAETVIVASTRVLHGTTLVKIK
                     GALKRTTNAAGLVAYKIGRTFRHFGHNGPTKKILPPASATSTTTGTPPNTTTTTTIPE
                     PAEVPISFTRSLNSTTSSTASSAIISPFISSSGSSIVSSIGSSFTFAIAERIVAPRLG
                     ATQFPLDAEVQDLPAGVPVVVQFTDPGPPTQGLTMARTVADVKHIQLTWGLLTGIVSL
                     LTFASSLDTPPLTADIRSFQFHETLSPRLTLAAAPREDTSLAEGADLYFFGTQAEAES
                     FAGRRIEFVKPDGASFEGVVTAVGPDASPATATGASLHRLTVSPEVVYKDFTNENPSV
                     VVHGNLVEATQGKSEREEPLGNGDARQVFQNFKLPKAPLTYLLSPGDTPPEVPELEIY
                     VNDRLWLRVASLFGRGADEQVYIVREDAAGDSWVQFGDGKTGARLPSGVKNVVARFRM
                     GTGAFGALKAETKAQAGARLDRLDKIQMPAPSTGGSEPESGDNAREAAPGKIQSLDRL
                     VSIRDFETETLAISGVERASAAWRIEENIPALHVTVLMQTGRAKEIDAVRQVLADFNR
                     CRGPRRFPVVVDEGQLLHVGVRLVYGLAPTFRADVVTPAIMSALGVTGAGTENRRGLF
                     GTLRRRFGEREYATRIEGTVQNVEGVVWAKVTSLVALGESPDPPVPVTVVECGQTELL
                     ALRAQDFTVGVAAEQTGEAC"
     gene            2109..>2715
                     /locus_tag="AC159P_22320"
     CDS             2109..>2715
                     /locus_tag="AC159P_22320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017296926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3698235.1"
                     /translation="MAEGKRVPLYERLPEIYRTKDAEASAPGQLRHYLALVEEAFGHI
                     HANIEELYDDLFVETSDDWVVPYIGDLLGTSLLKGDPWTLRADVADTIALRRRKGTLG
                     AVELLAYNLTRWGAHCVEMRERMVWNQHLNHQRPDEGGAPPYGLPSVRRQTVIRGGTV
                     TLRDPAMLSLLGTPFDPFAHTADVKPPAFGGVRYNLPNLAVF"
BASE COUNT          454 a         1000 c          862 g          399 t
ORIGIN      
        1 gagctcaagg agggcgaccg cctgctcgtt ggcaattcgg tgctcttctc gcccggtcgc
       61 atcgacaacg ccgagaccgt catcgtcgcc tcgacgcgcg tgctccacgg cacgacgctc
      121 gtcaagatca aaggcgcctt gaagcgcacg accaatgcgg cgggcctcgt cgcctataaa
      181 atcggtcgca ccttccgcca cttcggccac aacgggccga cgaaaaaaat tctcccgccg
      241 gccagcgcca cctccaccac tactgggacg ccccccaaca cgactacgac gacgaccatc
      301 cccgagccgg ccgaggtgcc catcagcttt acccgttctc tgaacagcac cacctcgtct
      361 acagcctcgt ccgccatcat ctcgcccttc atctcatcca gcggctcgtc cattgtctcg
      421 tccatcggct cgtccttcac attcgccatt gccgagcgca tcgtcgcgcc gaggctcggc
      481 gcgacgcagt tcccgctcga cgcggaggtg caggacttgc ccgcaggcgt ccccgtcgtc
      541 gtgcagttca cagaccccgg cccccccacg caggggttga cgatggcgag aaccgtcgcc
      601 gacgtgaaac acatccagtt gacgtgggga ctgttgacgg gcatcgtgag tctactgacg
      661 ttcgcctcct cgctcgacac gccgccgctc accgccgaca tccgcagctt ccaatttcac
      721 gagacgctca gcccgcggct cacgctcgcc gccgcgccgc gcgaagacac gtcgctcgcg
      781 gaaggcgccg acctctactt cttcggcacg caggctgagg ccgaatcctt cgcgggccgt
      841 cgcatcgagt tcgtgaaacc ggacggcgcg tcgttcgaag gcgtcgtcac cgcggtcggg
      901 ccggacgcct cgcccgcgac ggccacgggc gcgtcactgc acaggctcac cgtcagcccc
      961 gaagtcgtct acaaagactt caccaacgag aacccgtcgg tcgtcgtcca cggcaacctc
     1021 gtcgaagcga cgcaaggcaa gagcgagcgc gaagagccgc tcggcaacgg cgacgcgcgg
     1081 caggtcttcc agaacttcaa gctgccgaag gcgccgctca cctatctgct ctcgcccggc
     1141 gacacgccgc ccgaagtacc cgagcttgaa atctacgtca acgaccggct gtggctgcgc
     1201 gtcgcttcgc tcttcggtcg cggggcggac gagcaggtct acatcgtgcg cgaggacgcg
     1261 gcgggcgata gctgggtgca gttcggcgac ggcaagacgg gtgcgcgtct cccgtcgggc
     1321 gtcaagaacg tggtcgcgcg gttccgcatg gggacgggag cgttcggcgc gctgaaagcc
     1381 gagacgaagg cgcaggcggg cgcgcggctc gaccgcctcg acaaaatcca gatgcctgcg
     1441 ccgagcacgg gagggagcga acccgagagc ggcgacaacg cgcgcgaggc cgcgcccggc
     1501 aagattcaga gcctcgaccg cctcgtcagc atccgcgact tcgagaccga gacgctggcc
     1561 atctccggcg tcgagcgcgc gtcggcggcg tggcgcatcg aggagaacat ccccgcgctt
     1621 cacgtcaccg tgctgatgca gacgggccgc gccaaagaga tcgacgcggt gcgacaggtg
     1681 ctcgcggact tcaaccgctg ccgcggcccg cgccgcttcc ccgtcgtcgt ggacgagggg
     1741 cagctcctgc acgtcggcgt ccgtctcgtc tacggcctcg cgccgacctt ccgcgcggac
     1801 gtggtcacgc cggccatcat gtccgcgctc ggcgtcacgg gcgcgggcac agaaaaccgg
     1861 cgcgggctgt tcggcacgct ccgccggcgc ttcggcgagc gcgagtacgc gacgcgcatc
     1921 gaggggactg tgcagaacgt cgagggcgtc gtgtgggcga aggtgacttc gctcgtcgcg
     1981 ctcggcgagt cgcccgaccc gcccgttccc gtcacggtcg tcgagtgcgg tcagaccgag
     2041 ttgctcgcgc tccgcgcgca ggacttcacc gtcggcgtcg ctgccgaaca gacaggggag
     2101 gcgtgctgat ggcggaaggc aagcgagtcc cgctctacga aaggctgccg gagatttacc
     2161 ggacgaagga cgcagaggcg tccgcgcccg gccaactgcg ccactacctc gcgctcgtcg
     2221 aggaagcctt cggccacatc cacgccaaca tcgaagagct gtacgacgac ctcttcgtcg
     2281 agacctcgga cgactgggtc gtgccctaca tcggcgacct gctcggcacg agcctcctca
     2341 agggcgaccc gtggacgctt cgcgcggacg tagccgacac catcgcgctg cgccgccgca
     2401 aaggcacgct cggcgccgtc gagctgctcg cctacaacct gacgcgctgg ggcgcgcact
     2461 gcgtcgagat gcgcgagcgg atggtctgga accagcacct caaccaccag cgaccggacg
     2521 agggcggcgc gccgccttac ggcctgccga gcgtgcgccg ccagaccgtc atccgcggcg
     2581 gtaccgtcac cttgcgcgac ccggccatgc tctcgctgct cggcacgccc ttcgacccgt
     2641 tcgcacacac ggcggacgtg aagccgccgg ccttcggcgg cgtccgctac aacctgccga
     2701 atctcgccgt cttcc
//
LOCUS       DBNGOH010001151         4758 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3112082, whole genome shotgun sequence.
ACCESSION   DBNGOH010001151 DBNGOH010000000
VERSION     DBNGOH010001151.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4758)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4758)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4758
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3112082"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(384..1412)
                     /locus_tag="AC159P_22325"
     CDS             complement(384..1412)
                     /locus_tag="AC159P_22325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007328718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HIR3698236.1"
                     /translation="MSETESTLPPNQQPAAPGRWPVVGERAPRRSDEPWRVSVSGLVA
                     APKTWTLDEIAAMPRVEQAVDIHCVTRWSKLGAKFSGVPLARLLGECRPLPEARFLSF
                     VARSERGHSTSLPLAEAARAGALVALRYEGVPLEEAHGGPVRVVVPGRYFYKSVKWLE
                     RVEVLAEDRLGYWEQEAGYHNEADPWREQRYIAPNLDRRALADALARRDFRDKDFRSL
                     DATGLELLGLDARGALLRDADFRGVSLAGARFDGANLSNAHLEGANLRGASFVGADVE
                     GADFRGADLRGADFTRASMIAATFVDEADVATFARIDRTTVIEESSVEMLTPAQQSFV
                     RQALARNA"
     gene            complement(1417..2745)
                     /locus_tag="AC159P_22330"
     CDS             complement(1417..2745)
                     /locus_tag="AC159P_22330"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3698237.1"
                     /translation="MNPRPASVIILGGALLAACAAVYLWGLLAALVAAACGAAGFAAA
                     RLAGERPPNRSPEGMADAFPLHSVERSEVPAATVATAVAAGEVPAGALLEATMDSMRE
                     GVLVIDSAMRVVASNPSARDIFGTGGEAALRRRRLSEVTRSPAVYSAFAAAVARGERA
                     EVKFEATRASGARRVFDLHVAPLRQQEVVYGSDVRGAVGVFFDITQLERLERVRQEFL
                     SNVSHELRTPLTAIITFVETLEDGAVDDPETSRRFLNVIRRNSERMRSLINDILELSA
                     IEAGTVRVEPERLRLASLVGECFTALAALAAERRVSLVNEVAPEVLVYADARRLEQML
                     TNLVDNAIKFSAEGGAVRVAHERAEGRDRVHVGDDGEGIAPEHLPRIFERFYRVDRAR
                     SRALGGTGLGLAIVKHLARAHGGEASVRSAPGEGSTFTVELPASPESVVQ"
     gene            complement(2851..3567)
                     /locus_tag="AC159P_22335"
     CDS             complement(2851..3567)
                     /locus_tag="AC159P_22335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014098585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR3698238.1"
                     /translation="MAKPVLIVEDDPDIAEGLRYNLQREGLETRVALTGEQGLSAALD
                     PKGPPALVILDLMLPGMSGTELCRRLRREPATRRTPIIMLTARTSEADRVAGLDLGAD
                     DYISKPFSVRELMARVRAVLRRSDESAAARYEDNRLAIDFADMRVTCDGEAVRLTRKE
                     FSLLATLARGAGRVATRQQLLDNVWGYEYYGDTRTLDVHIRRLRQKLGPCGDAIETVV
                     GVGYRFIGCTETKEGLRTED"
     gene            complement(3629..4288)
                     /gene="phoU"
                     /locus_tag="AC159P_22340"
     CDS             complement(3629..4288)
                     /gene="phoU"
                     /locus_tag="AC159P_22340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HIR3698239.1"
                     /translation="MELHRRLDADLDLLRDRVLLLGGETEAALQRAMYALAERDSAAA
                     RDVLEGDDEIDQLEIEIDRLCIDVLALRQPAARDLRFVVAVAKITPILERIADHACNI
                     ARAAIDLNDEPQLKPYIDLPRMADSASRMLRSALDAFTNGDAAAARTLIARDDEIDEI
                     YDRLFHELITLMAQDPTTTTRAARLLFVGKHLERIGDYVTDICELTVYMTEAAIIKHS
                     N"
     gene            complement(4446..>4758)
                     /locus_tag="AC159P_22345"
     CDS             complement(4446..>4758)
                     /locus_tag="AC159P_22345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011554778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HIR3698240.1"
                     /translation="SGGQQQRLCIARALAVDPDVVLMDEPASALDPIATQRIEELVYD
                     LKKTYTIVIVTHNMQQAARVSDTTAFFWLGKLVEVNSTERMFTNPDEKLTEDYITGRF
                     G"
BASE COUNT          757 a         1603 c         1632 g          766 t
ORIGIN      
        1 tgaccttctg gcccacttta aaacccacca cacacctcct gtgacttaca aaaaacaagg
       61 ccgcgtctcg cacgtcgggt cttgggaaaa cccctgaggg ttcgtggcga cgcggccagc
      121 gtcggtgatt tctaacgaaa gtgtaacaag ccgcgcgggc gccggtcaag cttcaagatt
      181 tggcgcgaaa actcgatgaa atatcgtgtc aaaaatgcaa cgaatcggga cgcgaccgac
      241 ggcgacgggg cgacaaaggg acggggtgac ggggcgacca agaagacggg taatgcgaag
      301 aagcggcgcc ttcatcaatc atcaccccgt cactccgtct ccttgtctcc ccgtcccgtt
      361 cattccgtaa tgcgaaatcc gcgtcaagca tttcgcgcga gtgcttgccg gacgaacgac
      421 tgttgcgcgg gcgtcagcat ctcgacggac gactcttcga tgacggtcgt gcggtcgatg
      481 cgtgcgaagg ttgctacgtc cgcttcgtca acgaacgtcg cggcgatcat cgaggcgcgc
      541 gtgaagtccg cgccgcgaag gtctgcgccg cgaaagtccg cgccctccac gtccgcgccg
      601 acgaacgagg cgccgcgcag gttcgcgcct tcgaggtgcg cgttcgagag gttcgcgccg
      661 tcgaagcgtg cgcccgcaag gctgacgcca cggaagtctg catcgcgcaa gagcgcgccg
      721 cgcgcgtcga gtcctaaaag ttcgagtccg gtcgcgtcga ggctgcggaa gtctttgtct
      781 cggaagtcgc ggcgcgcgag cgcgtccgcc agcgcccggc ggtcgaggtt gggcgcgatg
      841 tagcgttgct cgcgccacgg gtcggcctcg ttgtgatagc ccgcctcctg ctcccagtag
      901 ccgaggcggt cttcggccag cacctcgacg cgctccagcc acttcacgct cttgtaaaag
      961 tagcggcccg gcacaacgac gcgcaccggc ccgccgtgcg cctcttcgag cggcacgcct
     1021 tcgtagcgaa gcgcgacgag ggcgcccgcg cgggcggctt ccgcgagcgg gagcgaagtg
     1081 ctgtggccgc gctcgctccg cgcgacgaag gagaggaagc gcgcttcggg cagcggacgg
     1141 cactcgccca gcaggcgcgc gagcggcacg cccgaaaact tcgcgccgag cttcgaccag
     1201 cgcgtgacgc agtggatgtc aaccgcctgc tcgacgcgcg gcatcgcggc gatctcgtcc
     1261 agcgtccacg tcttcggcgc ggcgacgagt cccgagaccg agacgcgcca cggctcgtcg
     1321 ctccgccgcg gcgcgcgctc gccgacgacg ggccaacggc ccggcgccgc cggttgttgg
     1381 ttgggaggta aagtgctctc cgtctcgctc atggcgctat tgaacaacgg attcgggcga
     1441 agccggaagc tcgacggtga aggtcgagcc ttcgccgggg gcggagcgca cgctggcctc
     1501 gccgccgtgc gcgcgcgcga ggtgcttgac gatggcgagg ccgaggcccg tgccgccgag
     1561 cgcgcgcgag cgtgcgcggt cgacgcggta gaagcgctcg aagatgcgcg ggaggtgttc
     1621 gggggcgatg ccctcgccgt cgtcgccgac gtggacgcgg tcgcgcccct cggcgcgctc
     1681 gtgcgcgacg cgcaccgccc cgccctccgc gctgaacttg atggcgttgt ccacgaggtt
     1741 cgtgagcatc tgttcgagcc gccgcgcgtc ggcgtagacg agcacttccg gcgccacctc
     1801 gttgacgagc gagacgcgcc gctcggcggc gagcgcggcg agcgctgtga agcactcgcc
     1861 cacgagcgag gcaaggcgca gccgctccgg ctcgacgcgc accgtcccgg cttcgatggc
     1921 cgacagctcg aggatgtcgt tgatcaggct gcgcatccgc tcggagttgc ggcggatgac
     1981 gttgaggaag cggcggctcg tctcggggtc atcgaccgcc ccgtcttcga gcgtctcgac
     2041 gaaggtgatg atggccgtca gaggcgtccg cagctcgtgc gagacgttcg agaggaactc
     2101 ctgccttacc cgttcgaggc gttcgagctg cgtgatgtcg aagaagacgc cgaccgcgcc
     2161 gcgcacgtcg ctgccgtaga cgacctcctg ctgccggagc ggcgcgacgt gcaggtcgaa
     2221 gacgcggcgc gcgccgctcg cgcgcgtcgc ctcgaacttg acctcggcgc gctcgccgcg
     2281 cgcgaccgcc gccgcgaacg ccgagtagac cgccggcgag cgcgtcacct cgctcagccg
     2341 ccggcggcgc agagccgcct cgccgcctgt cccgaagatg tcgcgggccg aagggttcga
     2401 cgccaccacg cgcatcgcgc tgtcgatcac cagcacgccc tcgcgcatgc tgtccatcgt
     2461 cgcttccagc agcgcgcccg ccggcacctc gcccgccgcc accgccgtcg ccaccgtcgc
     2521 cgcgggcacc tccgaacgct ccaccgagtg cagcgggaag gcgtcggcca tcccctcggg
     2581 cgatctgttc ggcgggcgct cgcccgcgag gcgcgccgcc gcgaagcccg ccgcgccgca
     2641 cgccgccgcg acgagcgcgg ccaacaggcc ccacaagtaa acggcggcgc acgccgccag
     2701 cagtgcgccg cccaggatga tcacagacgc cggacgcgga ttcataaaga aggtgaatag
     2761 taaatggtca atgatgaata gagaagcgct tctctattca tcattgacca tcgaccattc
     2821 atcagcacgg cgctcgtcaa ccgcttgcac tcagtcctca gtcctcagtc cttctttcgt
     2881 ttcggtgcag ccgatgaagc ggtagccgac gccgacgacc gtctcgatgg cgtcgccgca
     2941 ggggccgagc ttctggcgca gccgccggat gtgcacgtcg agcgtgcgcg tgtcgccgta
     3001 gtattcgtag ccccagacgt tgtcgagcag ttgctgccgc gtggcgacgc ggcccgcgcc
     3061 gcgcgcgagc gtggccagca gcgagaactc cttgcgcgtc aggcgcacgg cctcgccgtc
     3121 gcaggtgacg cgcatgtcgg cgaagtcgat ggccagccgg ttatcttcgt agcgcgccgc
     3181 cgccgactcg tccgagcggc gcaagacggc gcgcacgcgc gccatcagct cgcgcaccga
     3241 gaagggcttc gagatgtagt cgtcggcgcc gaggtcgagg cccgcgacgc ggtcggcctc
     3301 ggacgtgcgc gcggtgagca tgatgatggg cgtgcgccgc gtcgccggct cgcgcctgag
     3361 acggcggcac agctcggtgc cgctcatccc cggcagcatc aaatcgagga tgacgagcgc
     3421 gggcggcccc ttcgggtcga gcgccgcaga gaggccctgc tcgcccgtca gcgccacgcg
     3481 cgtctccaac ccttcgcgct gaaggttgta gcgcagcccc tccgcgatgt cggggtcgtc
     3541 ttcgacgatg agaacgggtt tagccatagg aaccgtgaac cgtaaaccgt gaagagtcgg
     3601 aaccgtttct aacccgtcac ggtttacgtt agttgctgtg tttgatgatc gccgcctccg
     3661 tcatgtagac ggtcagctcg cagatgtccg tgacgtagtc gccgatgcgt tcgaggtgtt
     3721 taccgacgaa gaggaggcgc gcggcgcgcg tcgtggtcgt cgggtcctgc gccatgagcg
     3781 tgatcagctc gtggaagagg cggtcgtata tctcgtcgat ctcgtcgtcg cgggcgatga
     3841 gcgtgcgcgc ggcggcggcg tcgccgttgg tgaaggcgtc gagcgccgag cgcagcatcc
     3901 gcgaggcgct gtcggccatc cgcggcaggt cgatgtaggg cttgagctgc ggctcgtcgt
     3961 tgaggtcgat ggcggcgcgc gcgatgttgc acgcgtggtc ggcgatgcgt tcgaggatcg
     4021 gcgtgatctt ggcgacggcg acgacgaacc gcaggtcgcg cgcggcgggc tgccgcagcg
     4081 cgagcacgtc gatgcagagg cggtcgatct cgatctccaa ctggtcgatc tcgtcgtcgc
     4141 cttcgagcac gtcgcgcgcc gccgccgagt cgcgctcggc cagcgcgtac atcgcccgct
     4201 gcaaagccgc ctccgtctcg ccgcccagca ggagcacgcg gtcgcgcagc aggtcgaggt
     4261 cggcgtcaag tcttctgtgt agttccatgg tagtaagctg ttagctgttg gcgattagct
     4321 gttcgcgcac ggcagccgcc gtgacaggag tcgcgccctg catttacagg ggatgccgga
     4381 caacactctc cgcatcgatt ccatgataaa gctgaaagct aacggctgac agctgatcgc
     4441 tccaatcacc cgaagcgccc cgtgatgtag tcctcggtca gcttctcgtc ggggttggtg
     4501 aacatcctct cggtcgagtt cacttcgacg agcttgccga gccagaagaa ggccgtcgtg
     4561 tcggagacgc gcgcggcctg ctgcatgttg tgcgtcacga tcacgatggt gtaggtcttc
     4621 ttcaagtcgt acaccagctc ctcgatccgc tgagtcgcga tcggatcgag cgccgacgcc
     4681 ggctcgtcca tcaggacgac gtcgggatcg acggccagcg cgcgggcgat gcagagccgc
     4741 tgctgctgtc cacccgag
//
LOCUS       DBNGOH010001152         4016 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1095920, whole genome shotgun sequence.
ACCESSION   DBNGOH010001152 DBNGOH010000000
VERSION     DBNGOH010001152.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4016)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4016)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4016
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1095920"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            47..880
                     /locus_tag="AC159P_22350"
     CDS             47..880
                     /locus_tag="AC159P_22350"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR3698241.1"
                     /translation="MDLSELTKLYDFKGRTVVVTGATGVLCGAMAEALAGCGANVAAL
                     ARSEEKGAALLSRLKGPGRHAVIAGDVLDQASLEAAARRVKEEFGGVDALVNGAGGNS
                     PQATARPPDLTFFDLPEDALRYVFDLNLLGTILPTQVFGRLMAEAGEGVILNVSSMSA
                     MRPLTRVVGYSAAKAGINSFTQWLAVHLAQEYSPRLRVNAVAPGFFVTEQNRSLLTNP
                     ETGELTPRGQTIIAHTPMNRFGSPEDLLGATLWLLSPASAFVTGIVVPVDGGFSAFGG
                     V"
     gene            1014..2654
                     /locus_tag="AC159P_22355"
     CDS             1014..2654
                     /locus_tag="AC159P_22355"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009821770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HIR3698242.1"
                     /translation="MLKTVATTPFTDQRPGTSGLLKKTRVFMQPNYLENFVQSVFDAV
                     RETTGADFSGETLVVGGDGRYYNREAAQTIIRMAAANGFARVLVGRGAILSTPAVSAL
                     VRRRGALGALVLSASHNPGGPAEDFGIKYNVRNGGPAPEAVTEAIYQKTLTLSEYRTL
                     DSLDVDLDSEGAFKVGEAEVLVIDSLEDYTAVMEELFDFDSLRALFGAGFRMTFDAMS
                     AVTGPYARHILEERLGAPAGTVINGTPLEDFGGHHPDPNLVHAAELVERLSSADAPDF
                     GAACDGDGDRNLILGRNFFVSPGDSLAVIAEHAQNSIPGYREGLAGVARSMPTSTAAD
                     RVAAALGVRCYETPTGWKFFGNLLDAGLCTLCGEESFGTGSDHVREKDGLWAVLCWLS
                     ILAATRKGVEEVVREHWRRFGRSYYQRHDFEGLDADAAGRMVEALREKLPTLPGTAFA
                     GGEIATADDFAYTDPVDGSTSVRQGVRLLLADGSRVVCRLSGTGTSGATLRLYVERYR
                     DDGGVAETDDVLAPLVRAAKDLLSLREYCGRDEPNVIT"
     gene            2967..>4016
                     /locus_tag="AC159P_22360"
     CDS             2967..>4016
                     /locus_tag="AC159P_22360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012411605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HIR3698243.1"
                     /translation="MLVGQGYEDRFLDEPEIRELMRSALAGTETEGRRVLVIIPDSTR
                     TAPIPLMFRLFHEQLAGKTAALDYLIALGTHMMMSDEAINKLVGVTAEEREGKYAGVR
                     IFNHRWDLPETFADLGRITADEIARITGGLMHRDVDVRLNKLVFDYDQIVICGPVFPH
                     EVVGFSGGNKYFFPGIGGPEVINFSHWLGAVITSYSVIGTKYTPVRRVIDRAASFIDR
                     PKLCFAMVVKGDGLAGLYVGTPEEAYEAAADLSSKLHIKWVEKPYERVLAVMPKMYDD
                     LWTAAKGMYKLEPAVADGGEVVIYAPHVEEVSYTHGHLLDEVGYHVRDYFLKQWDRFK
                     DYPGGVLAHSTHLRGM"
BASE COUNT          663 a         1355 c         1342 g          656 t
ORIGIN      
        1 cggcggcaac ggcgtcggaa tctgaggtcg gcagcgaggg ttgaagatgg acttgtcgga
       61 actgacaaag ctctacgact tcaaggggcg caccgtcgtc gtcacgggtg cgacgggcgt
      121 gctctgcggc gcgatggccg aggctctggc cgggtgcggc gcgaacgtcg cggcgctcgc
      181 gcgcagcgag gagaagggtg cggcgctgct gtcgaggctc aaggggccgg gccgccacgc
      241 cgtcatcgcg ggcgacgtgc tcgaccaggc ttcgctcgaa gcggcggcgc ggcgcgtgaa
      301 ggaggagttc gggggcgtgg acgcgctcgt caacggcgcg ggcggcaaca gcccgcaggc
      361 gacggcgcgc ccgcccgacc ttaccttctt cgacctgccg gaggacgcgc tgcgctacgt
      421 cttcgacctc aacctgctcg gcactatctt gccgacgcag gtcttcggtc ggctgatggc
      481 cgaggcgggc gagggcgtca tcctgaacgt ctcttcgatg agcgcgatgc gaccgctgac
      541 gcgcgtcgtc ggctattcgg cggcgaaggc cggcatcaac agcttcacgc agtggctcgc
      601 cgtccatctc gcgcaggagt atagccctcg cctgcgcgtc aacgccgtcg cgcccggctt
      661 cttcgtcacc gagcagaacc ggagtttgct gacgaacccc gagaccggcg agctgacgcc
      721 gcgcggccag accatcatcg cgcacacgcc gatgaaccgc ttcggctcgc ccgaagacct
      781 gctcggcgcg acgctctggc tgctctcgcc cgcctccgcc ttcgtcaccg gcatcgtcgt
      841 acccgtggac ggcggcttct cggccttcgg cggggtgtga tgggagctat cagctattag
      901 ctgttagctc ttagctgttc gattgatttg acgcttgata caagcgccgc ccccgtcaac
      961 ctttatcttt tattccaaaa gcgaacggct aacggctaac agctaaaggc ttcattttga
     1021 aaacagtcgc caccacgccg ttcacggatc agcgccccgg cacctcgggg ctgctgaaga
     1081 agacgcgcgt cttcatgcag ccgaactacc tggagaactt cgtccagtcg gtcttcgacg
     1141 cggtgcgcga gacgacgggc gcggacttct cgggcgagac gctggtggtc ggcggcgacg
     1201 gtcgctacta caaccgcgag gccgcgcaga ccatcatccg catggccgcg gccaacggct
     1261 tcgcgcgcgt gctcgtcggg cgcggcgcga ttctctcgac gcccgccgtc tcggcgctcg
     1321 tccgccgccg cggcgctctg ggcgccctcg tcctctccgc gagccacaac cccggcggcc
     1381 ccgccgaaga cttcggcatc aagtacaacg tccgcaacgg cggccccgcg cccgaggccg
     1441 tcaccgaagc gatttatcaa aagacgctca ccctcagcga gtaccgcacg ctcgacagcc
     1501 tcgacgtaga cctcgacagc gaaggcgctt tcaaagtcgg cgaagcagaa gtcctcgtca
     1561 tcgactcgct cgaagactac acggcggtga tggaagagct gttcgacttc gactctttgc
     1621 gcgccctctt cggcgcgggc ttccgcatga cgttcgacgc gatgagcgcc gtcaccggcc
     1681 cctacgcgcg ccacatcctc gaagaacgct tgggggcgcc tgcgggcacc gtcatcaacg
     1741 gcacgccttt ggaagacttc ggcggtcatc atccggaccc gaacctcgtc cacgccgccg
     1801 agctggtcga acgtctctct tccgcggacg cgcccgactt cggcgcggcg tgcgacggcg
     1861 acggcgaccg caacctcatc ctcggtcgca acttcttcgt ctcgccgggc gactctctgg
     1921 cggtcatcgc cgagcacgcg caaaacagca tccccggcta ccgcgaagga ctcgcgggcg
     1981 tcgcgcgctc gatgccgacc tcgaccgccg ccgaccgtgt cgccgccgcg ctcggcgtcc
     2041 gctgttacga gacgccgacc ggctggaagt tcttcggcaa ccttttggac gcgggtctct
     2101 gcacgctctg cggcgaggag agcttcggca ccggctccga ccacgtgcgc gagaaggacg
     2161 ggctgtgggc cgtgctctgc tggctctcga tactggccgc gacgcgcaag ggcgtcgaag
     2221 aggtcgtgcg cgaacactgg cggcgcttcg ggcgcagcta ctaccagcgg cacgacttcg
     2281 aagggctgga cgcggacgcc gccgggcgga tggtcgaagc gttgcgcgag aagctgccga
     2341 ccctgcccgg caccgcgttc gcgggtggag agatagcgac tgccgacgac ttcgcttaca
     2401 cggaccccgt ggacggcagc acctcggtgc ggcagggcgt ccgcctcctg ctcgcggacg
     2461 gctcgcgcgt cgtctgccgc ctgtcgggca cgggcacgtc gggcgcgacc ctgcgcctct
     2521 acgtcgagcg ctaccgcgac gacggcggcg tcgccgagac ggacgacgtg ctggcgccgc
     2581 tcgtgcgcgc cgcgaaggat ttactctcct tgcgcgaata ctgcgggcgc gacgagccga
     2641 acgtcatcac gtgaggattg cggattttgg attagaagaa gcgttgacag ttgacgggag
     2701 ccgccgtgtt gcttcgccgc gtagcggcga ttgaaggtag cccggcgttt caacgccggg
     2761 tcgggcgcgg acgagcggcg ccgcctcgcg tcagcgacgc ctgaatggac ggcgcaaggt
     2821 tcacgcgtcg ctacgcgacg ccgacggttg gccgacgcca atcccggcct tgaaaggccg
     2881 ggctaaactc aatcgccgct ccgcggcgaa agacatcgaa tcacttacaa tcgccgctcc
     2941 gcggctgtgt cgagaggtgc cgaaggatgc tggttggtca aggctacgaa gacaggtttc
     3001 tggacgagcc ggagattcgt gagttgatgc ggtcggcgct cgcgggcacc gagaccgagg
     3061 gccgccgcgt tctcgtcatc atccccgact cgacgcgcac ggcgccgatc ccgctcatgt
     3121 tccgcctctt ccacgagcag ctcgcgggca agacggcggc gctcgactac ctcatcgcgc
     3181 tcggcacgca catgatgatg agcgacgagg cgatcaacaa actggtcggc gtgacggcgg
     3241 aggagcgcga agggaagtac gccggggtgc gaatcttcaa ccaccgctgg gacttgcccg
     3301 agacgttcgc ggacctcggg cgcatcacgg cggacgagat cgcgcgcatc acgggcgggc
     3361 tgatgcaccg cgacgtggac gtgcggctga acaagctggt cttcgactac gaccagatcg
     3421 tcatctgcgg cccggtcttc ccgcacgagg tggtgggctt ctcgggcggc aacaagtatt
     3481 tcttccccgg catcggcggc cccgaggtca tcaacttctc gcactggctc ggcgccgtca
     3541 tcacgagcta ttcggtgatc ggcacgaagt acacgcccgt caggcgcgtc atcgaccggg
     3601 cggcatcctt catcgaccgg cccaagctct gcttcgcgat ggtcgtcaag ggcgacggac
     3661 tcgccggtct ttacgtcggc acgcccgagg aggcttacga ggcggctgcc gacctctctt
     3721 cgaagctcca catcaagtgg gtcgagaagc cttacgagcg cgtgctcgcc gtgatgccga
     3781 agatgtacga cgatttgtgg acggcggcga agggaatgta caagctggag ccggcggtgg
     3841 cggacggcgg cgaggtcgtc atctacgcgc cgcacgtcga ggaggtcagc tacacgcacg
     3901 ggcacctgct cgacgaagtc ggctaccacg tgcgcgacta cttcctgaaa cagtgggaca
     3961 ggttcaagga ctaccccggc ggcgtgctcg cgcacagcac gcacctgcgc gggatg
//
LOCUS       DBNGOH010001153         3970 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2744404, whole genome shotgun sequence.
ACCESSION   DBNGOH010001153 DBNGOH010000000
VERSION     DBNGOH010001153.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3970)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3970)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3970
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2744404"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..239)
                     /locus_tag="AC159P_22365"
     CDS             complement(<1..239)
                     /locus_tag="AC159P_22365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HIR3698244.1"
                     /translation="MISLAVVNCAQLVTLRGPARPRVGAEMRELSVVADGAMLVRGGR
                     IERVGTRGEIEPLVKGECEVFDAGGRVVLPGFVDA"
     gene            complement(323..790)
                     /locus_tag="AC159P_22370"
     CDS             complement(323..790)
                     /locus_tag="AC159P_22370"
                     /inference="COORDINATES: protein motif:HMM:NF024865.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YybH family protein"
                     /protein_id="HIR3698245.1"
                     /translation="MNRRILYAAVPLAAAVAVVFGYQGRLPKAGKQDKEIAAVRAVIE
                     AQAEAWNRGDIEGFMAGYAKEDSITFVSGDTVTRGWQTVLDRYRKNYDSRAKMGTLTF
                     SELEFKPLSEFYVMAVGRYELARDGDRPRGRFTLVFRRTTGGWRIVHDHTSSA"
     gene            complement(846..1544)
                     /locus_tag="AC159P_22375"
     CDS             complement(846..1544)
                     /locus_tag="AC159P_22375"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014305.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR3698246.1"
                     /translation="MTPDNTPHTERTISAETKLYYDLDAPLGGPAPLLIALHGYGSSK
                     RWMMREAREHAPEGFAVAALQGPHQHIKEPKEQGGPLRYGFGWLTNFHPEDAVALHHR
                     ALLDLIEALVGEGVARRGQVFLMGFSQTCALNFRFAFTHTKTLRGVVGICGGMPGDWE
                     TSEVYQPTGASVLYLHGTRDEFYTPERVAGYGERLRERARDVEVKAYEVGHEFAPAMR
                     GDVRAWLAARAAGP"
     gene            complement(1531..2289)
                     /locus_tag="AC159P_22380"
     CDS             complement(1531..2289)
                     /locus_tag="AC159P_22380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3698247.1"
                     /translation="MKSARAVNVESDRPATLKGVAADAVRLLTFRSTREELARFGLRH
                     LAFGIFCTWLVGMGRYWDDPRANLAQHLGVGSVVYVFALAFVVWLVVLPLRPARWSYL
                     NVCAFVALVSPPAILYAIPVERLFTLETAASLNVWFLAAVATWRVALLAFYLSRQAAL
                     GWLAVTVAGLLPLTAIVVSLTALNLERVAFNVMGGLRETTANDRAYAVLVSLTILSFL
                     LIVPLTVVYVALVVAARRSRALTQLSLTSEHDAR"
     gene            complement(2286..>3970)
                     /locus_tag="AC159P_22385"
     CDS             complement(2286..>3970)
                     /locus_tag="AC159P_22385"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024754.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HIR3698248.1"
                     /translation="AEVLLDIIGFATNTDRPISEVVAARHPYFEPLASEITRVDRVFM
                     ERKRERNVMDYDDLLLNWKRLLEEKDDIARLYQEQFEHVLVDEYQDTNKLQAEIVDLL
                     AAKHRNVMVVGDDAQSIFAFRGAEWSNIYEFPQRYPEARVYRLETNYRSTPEILAVAN
                     VSIAANRKQFPKRLRAARASLGLQPALVPCRDADQQAAFVAARVLELRDEGLSLQETA
                     VLYRSHYHSLELQLELTRRGIPYRVRSGVRFFEQAHIKDVVSYLRLVTNPRDELAWKR
                     VLRLVPNVGPATANRVWERIAYAPDPVALIVRGEADAAAAKRGTGWAEFRTLLEELSK
                     DEARNNPARQIEVILARGYEAYLEANYENAEARLEDLRQLAHYSARYNSTEEFLAELA
                     LLSTERYGAPQAVAGEDVVSGGDEDELLTLTSVHQAKGLEWRAVFILWAADGKFPSPR
                     SLRDAEGEDEERRLWYVALTRARDQLYISYPLMVTDYNRQTLVQRPSRFVTEVPPELY
                     EIWSLEEESALQSIPAYDEAAGAIDEGAAGEDDAAGDSEEGDEGKGGGGYVN"
BASE COUNT          623 a         1343 c         1362 g          642 t
ORIGIN      
        1 tgcgcgtcca cgaagcccgg cagcaccacg cggccccctg cgtcgaacac ctcgcactcg
       61 cctttgacca gcggctctat ctcgccgcgc gtcccgacgc gctcgattct cccgccgcgg
      121 acgagcatcg cgccgtccgc gacgacggac agctcgcgca tctctgcgcc gacgcgcggg
      181 cgcgcggggc cgcgcagcgt gacgagctgc gcacagttga cgacggccag cgaaatcata
      241 agtccgaagt ccgaagtccg aagtccgaag tcaacccgtc gatgctctgt ttctgacttc
      301 agacttccga cttcagactt tcctaagccg aagaggtgtg gtcgtggacg atgcgccagc
      361 cgccggtcgt gcggcggaag acgagcgtga agcgaccgcg cgggcggtcg ccgtcgcgcg
      421 ccagctcgta acgcccgacg gccatcacgt agaactcgct caggggcttg aattcgagtt
      481 ccgagaaggt gagcgtgccc atcttcgcgc gcgagtcgta gttcttcctg tagcggtcga
      541 ggacggtctg ccagccgcgc gtcaccgtgt cgccggagac gaacgtgatc gagtcctctt
      601 tcgcgtagcc tgccatgaag ccttcgatgt cgccgcggtt ccacgcctcg gcttgcgcct
      661 cgatgacggc gcgcaccgcc gcgatctctt tatcctgctt gcccgccttc ggcagacggc
      721 cctggtagcc gaacaccacc gccaccgccg ccgcgagcgg caccgccgcg tacaggattc
      781 tacggttcat ctcgtcgggt tctcctttcc ggtctcgcga ggttacgcgg attcggcgcg
      841 cgacttcaag ggcctgcggc gcgcgccgcg agccacgcgc gcacgtcccc gcgcatggcg
      901 ggcgcgaact cgtggcccac ttcgtaggct ttgacctcca cgtcgcgggc gcgctcgcgc
      961 aatcgttcgc cgtaaccggc gacgcgctcc ggcgtgtaga actcgtcgcg cgtgccgtgg
     1021 aggtagagga cggaagcccc ggtcggctga taaacctcgc tcgtctccca gtcgccgggc
     1081 atcccgccgc agatgccgac gacgccgcgc agcgtcttcg tgtgcgtgaa ggcgaagcgg
     1141 aagttgagcg cgcacgtctg cgaaaagccc atcaggaaga cctgcccgcg ccgcgcgacg
     1201 ccttcgccga cgagcgcttc gatcagatcg agcagcgccc ggtggtggag cgcgaccgcg
     1261 tcctccgggt ggaaattcgt cagccagccg aagccgtagc gcagcgggcc gccctgctcc
     1321 ttcggctcct tgatgtgctg gtgcggccct tgcagcgccg cgacggcgaa gccctcgggc
     1381 gcgtgctcgc gcgcctcgcg catcatccaa cgcttgctgc tgccgtagcc gtgcagcgcg
     1441 atcagcagcg gcgcggggcc gcccagaggg gcgtcgaggt cgtagtagag cttcgtctcg
     1501 gcgctgatgg tgcgttccgt gtgcggtgtg ttatcgggcg tcatgctctg aagttagcga
     1561 aagctgagtg agcgcgcgcg accttcgcgc ggcgacgacg agggcgacgt agacgaccgt
     1621 taaaggcacg atcagcagga acgaaaggat ggtcagcgag acgagcacgg cgtaggcccg
     1681 gtcgttggcc gtcgtctcac gcagcccgcc catgacgttg aaggccacgc gctcaaggtt
     1741 cagcgccgtg agcgagacga cgatggccgt cagcggcagc agccccgcga ccgtcaccgc
     1801 gagccacccg agcgccgcct gccgcgacag gtagaaggcg agcagcgcca cgcgccacgt
     1861 cgccaccgcg gcgaggaacc agacgttcag gctcgccgcc gtctccagcg tgaagaggcg
     1921 ctcgaccggt atcgcgtaga ggatggccgg cggcgagacg agcgcgacga acgcgcacac
     1981 gttcagatac gaccagcgcg cgggccggag cggaagcacg accagccaca cgacgaacgc
     2041 gagcgcgaag acgtagacga ccgacccgac gccgaggtgc tgcgcgaggt tcgcgcgcgg
     2101 gtcgtcccaa tacctgccca tcccgaccag ccacgtgcag aagatgccga aggcgaggtg
     2161 ccgaagaccg aaccgcgcca actcctcgcg cgtcgagcgg aacgtcagca gccgcacggc
     2221 gtccgccgcg acgcccttca gcgtcgcggg ccgatcactc tcgacgttga cggcgcgcgc
     2281 gctcttcaat tcacgtatcc tccgccgcct ttaccttcgt cgccttcttc gctgtcgccc
     2341 gccgcgtcgt cttcgccagc ggcgccttca tcaatcgccc ccgcggcctc gtcgtaagcc
     2401 gggatggact gcaacgcgct ctcctcttcg aggctccaga tttcgtacag ctcgggcggc
     2461 acctccgtga cgaagcgcga ggggcgctgg acgagggtct ggcggttgta gtccgtgacc
     2521 atgagggggt aggagatgta gagctggtcg cgtgcgcggg tgagggcgac gtaccagagg
     2581 cggcgctcct cgtcctcgcc ctcggcgtcg cgcagggagc ggggcgaggg gaacttgccg
     2641 tcggcggccc agaggatgaa gacggcgcgc cattcgagac ctttggcctg atggacggag
     2701 gtgagcgtca gcagctcgtc ttcgtcgccg ccggagacca cgtcttcgcc cgcgactgcc
     2761 tgcggggcgc cgtagcgctc ggtcgagagc aacgcgagtt cggccagaaa ctcttcggtc
     2821 gagttgtagc gcgcgctgta gtgcgcgagc tgccgcaagt cttcgagccg cgcctccgcg
     2881 ttctcgtagt tggcttcgag ataagcctcg tagccgcgtg cgaggatcac ctcgatctgc
     2941 cgcgcggggt tgttgcgcgc ctcgtccttc gacagctctt cgagcagcgt gcggaactcg
     3001 gcccagcccg tgccgcgctt cgcggcagcc gcgtccgcct cgccacgcac gatcaaagcg
     3061 acggggtcgg gcgcgtaggc gatgcgctcc cagacgcgat tcgcggtggc cgggccgacg
     3121 ttcgggacga ggcgcagcac gcgcttccac gccagctcgt cgcgcgggtt ggtgacgagc
     3181 cgcaggtaag agaccacgtc cttgatgtgc gcctgctcga agaagcggac gcccgagcgg
     3241 acgcggtagg ggatgccgcg acgcgtcagc tcgagctgta gttcgagcga gtgatagtgc
     3301 gagcggtaga gcaccgccgt ctcttgcagg ctcagcccct cgtcgcgcag ctccaacacg
     3361 cgcgcggcga cgaaggcggc ctgctggtct gcgtcgcggc aggggacgag cgcgggctgg
     3421 aggcccaggc tggcgcgcgc cgcccgcagc cgtttaggga actgcttgcg gttcgcggcg
     3481 atggagacgt tggcgacggc gagtatctcg ggcgtcgagc ggtagttggt ttcgagccgg
     3541 tagactcgcg cctcggggta gcgctgcggg aactcgtaga tgttcgacca ctcggcgccg
     3601 cggaaggcga agatcgactg cgcgtcgtcg ccgacgacca tcacgttgcg gtgcttcgcc
     3661 gccaagagat cgacgatctc cgcttgcagc ttgttcgtgt cctgatactc gtccaccagc
     3721 acgtgctcga actgctcctg atagaggcgc gcgatgtcgt ccttctcttc cagcagccgc
     3781 ttccagttca gcagcaggtc gtcgtagtcc atcacgttgc gctcgcgctt gcgctccatg
     3841 aagacgcggt caacgcgcgt gatctccgag gcgagcggct cgaagtaggg gtggcgcgcg
     3901 gcgaccactt cggagatggg ccggtcggtg ttggtggcga agccgatgat gtcgagcagc
     3961 acctcggctt
//
LOCUS       DBNGOH010001154         5030 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2376908, whole genome shotgun sequence.
ACCESSION   DBNGOH010001154 DBNGOH010000000
VERSION     DBNGOH010001154.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5030)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5030)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5030
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2376908"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..880
                     /locus_tag="AC159P_22390"
     CDS             <1..880
                     /locus_tag="AC159P_22390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilM"
                     /protein_id="HIR3698249.1"
                     /translation="LFAVKRAAFAQLPAGLLRPNFDESNVAGPAELAGVLAELVANVG
                     MSKRRRWSVALPEAATRTAILTMEGGAASRTESEEMVRWKTERAVGAPLDELRVARER
                     LPVDAAGRPRYLVSAVRLGVLAEYEAAFEALGWHAGLILPRHMGEAWWLMRGAARAPH
                     EDSLLVSSHNEGFTAVIMRAAQPLLVRNVICDVEDRADELYRFLLFYRDRNAPPAFEG
                     EEAIERAAPAESIERLLVAGTGLDAAEVGGIVAETLQVPPRMLGPADVRLSFPSNEID
                     FRHLAAPAGLAALAWA"
     gene            960..1571
                     /locus_tag="AC159P_22395"
     CDS             960..1571
                     /locus_tag="AC159P_22395"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR3698250.1"
                     /translation="MTAQAVSLKSTISVADSELVARAIAGREESFEELVRRYQRPIAA
                     YVYRMVGDYDAALDLTQEVFIRVYGSLARYRSEFKFSTWIYRIAHNAAIDHLRRCDNS
                     RTEDLKLESEGGQTYEKPLASGAPSPEQESEREERRAEIEGVVLNLPPAYRELIVLRH
                     AHDLSYDEIAEVTCLPLGTVKNRIFRAREAMRTLLVERGITSM"
     gene            1593..2195
                     /locus_tag="AC159P_22400"
     CDS             1593..2195
                     /locus_tag="AC159P_22400"
                     /inference="COORDINATES: protein motif:HMM:NF024881.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor family protein"
                     /protein_id="HIR3698251.1"
                     /translation="MNVAAADMHDAGRCGWERSVAAYLDGELDADASGAFEAHAKGCD
                     SCAAALREQRRLLCLLDNAFDETFSKKVALPADFTRVVKARAQTDMSGVRARRERTIS
                     LKICVGLACAIFALLGAPSLEALAPAADAARAAAGVVGVSAGAAADAGSGALVIARGV
                     GSRLVADSQLHALLQWLLFGFVLALLLLLIGRYHRARAGD"
     gene            2526..3461
                     /locus_tag="AC159P_22405"
     CDS             2526..3461
                     /locus_tag="AC159P_22405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="bactofilin family protein"
                     /protein_id="HIR3698252.1"
                     /translation="MKAATACVLLLLFFEASFAQDAPAHAPAAPSADELVIDEDYTGD
                     VFGVGRTVRVRGRVSHGVIAFGGDVVVEGRVEGDVATVGGSVSQREGSYVGGDVIILG
                     GTYHHGKTGPGRNPSSNTIMFAGYEEELRGLARDPSVLLTPEFTLAFVGLRLLGVLLW
                     FVVSLVLTAISPGAVSRGAARLQLTSLRVAVIGVLGALVAGPGVFAALHYLPPVLGVF
                     VSATALLLLLFAYVFGRVCIYAATGRWLQRLVLAEDKRSESIALLLGAVFWSVLLTLP
                     YVWPFVFAGLLVVSLGLSLTARHPLGRKRAAAAAA"
     gene            3583..4233
                     /locus_tag="AC159P_22410"
     CDS             3583..4233
                     /locus_tag="AC159P_22410"
                     /inference="COORDINATES: protein motif:HMM:NF014667.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HIR3698253.1"
                     /translation="MKTLLTVAFVCLAAAAVRAQPAPPAPKPITPPPDPHAAHKQQPP
                     AEEPTGAQKWFTDVELLTQDGAKVRFYSDVLKGKTVVVNAFFTTCTSVCPPMNRSMEK
                     IQEALGDRVGRDVFLVSITVDPVTDTPPRLKEYARKFHARAGWTFLTGKKENVDWALY
                     KLGQYVENKDDHKTVIIIGNETTGLWKKAFGLAKAEELIRLVEGVANDKGAAPPVK"
     gene            4362..>5030
                     /locus_tag="AC159P_22415"
     CDS             4362..>5030
                     /locus_tag="AC159P_22415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HIR3698254.1"
                     /translation="MNRLLFTLFIALVCAAPAAAQTQATQQQPPPPKPATVETLNAAE
                     AAMLCGCEGAPLPAVLAVVNGVKITPADLGPETAERVRQLQTEVIKARERELDLQINS
                     LLLDAEAKKRGVNTSKVIEDEIVSKATEPTDAEAQAFYDQNKERIQTGFADVKPQIVS
                     YLREQRQRDLAAKLAERLRAAAAVKTNVATVTQPAGPADRARVFATVNGRNITSGEVE
                     DSLRS"
BASE COUNT          758 a         1869 c         1668 g          735 t
ORIGIN      
        1 cctgttcgcc gtcaagcgcg ccgccttcgc ccaactcccc gcgggcctgc tccgccccaa
       61 cttcgacgag agcaacgtcg ccggcccggc ggagctggcg ggcgtgctgg ccgagctggt
      121 ggccaacgtc gggatgtcga agcggcggcg ctggtcggtc gcgcttcccg aggccgcgac
      181 gcgcaccgcc atcctgacga tggagggcgg cgccgcctcg cgcacagagt ccgaggagat
      241 ggtgcgctgg aagaccgagc gcgccgtcgg cgcgccgctc gacgagctgc gcgtcgcgcg
      301 cgagcggctg ccggtggacg ccgcgggccg cccgcgctac cttgtctccg ccgtccgcct
      361 cggcgtgctc gccgagtacg aggcggcctt cgaggcgctc ggctggcacg ccgggctgat
      421 cctcccgcga cacatgggcg aggcgtggtg gctgatgcgc ggcgccgccc gcgccccgca
      481 cgaagactcg ttgctcgtca gctcgcacaa cgaaggcttc accgccgtca tcatgcgcgc
      541 cgcgcagccc ctcctcgtcc gcaacgtcat ctgcgacgtg gaagaccgcg ccgacgagct
      601 gtaccgcttc ctgctcttct accgcgaccg caacgcgccg cccgccttcg aaggcgagga
      661 ggccatcgag cgcgccgccc ccgcggaatc gatcgagcgc ctgctcgtcg ccggcaccgg
      721 cctcgacgcc gccgaggtcg gcgggatcgt cgccgagacg ctgcaagtcc cgccgcgcat
      781 gctcggcccc gcggacgtgc gcctctcgtt cccctcgaac gagatcgact tccgccacct
      841 cgccgcgccc gccggcctcg ccgccctcgc ctgggcctga aaagcgcggg ccgcgcagcg
      901 ccgccgtgtc gcccgcgcct gaaactttcc tcgccgctcg cgcgtaacat cttggcatca
      961 tgactgcaca ggccgtctct ctgaaatcaa ccatctcggt ggccgacagc gagcttgtcg
     1021 cgcgcgcgat cgcggggcgc gaggagagct tcgaggagct ggtgcgccgc taccagcggc
     1081 ccatcgcggc ctacgtctat cgcatggtcg gcgattacga cgcggcgctc gatctgacgc
     1141 aggaggtctt catccgcgtc tacggctcgc tcgcgcgcta ccgctccgag ttcaagttct
     1201 cgacctggat ttaccgcatc gcgcacaacg cggccatcga ccacctgcgc cgctgcgaca
     1261 actcgcgcac cgaagacctc aagctcgaat cggagggcgg gcagacctac gagaagccgc
     1321 tggcgagcgg cgcgccctcg cccgaacagg agagcgagcg cgaggagcgg cgcgccgaga
     1381 tcgagggcgt cgtcctgaac ctgccgcccg cctaccgcga gctgatcgtg ctccgccacg
     1441 cgcacgacct cagctacgac gagatcgcag aggtgacgtg cctccccctg ggcacggtca
     1501 agaaccgtat cttccgcgcg cgcgaggcga tgcgcacgct cctcgtcgaa cgaggcatca
     1561 cgagcatgta ggggcgggga taatttttca ggatgaacgt cgcggcagca gacatgcacg
     1621 atgcagggcg ttgcgggtgg gagcgctcgg ttgccgccta cctcgacggc gagctggacg
     1681 ccgacgcctc cggcgccttc gaggcgcacg cgaaagggtg cgacagttgc gccgccgcgc
     1741 tccgcgaaca gcgacgcctg ctctgcctgc tcgacaacgc cttcgacgag acgttctcga
     1801 agaaggtcgc gctgcccgcg gacttcacgc gcgtcgtcaa ggcccgcgcg cagaccgaca
     1861 tgagcggcgt gcgcgcgcgg cgcgagcgca ccatctccct caaaatctgc gtcggcctcg
     1921 cctgcgccat cttcgcgctg ctcggcgcgc cttccttgga agcgctcgcg cccgcggcgg
     1981 acgcggcgcg cgcggcggcg ggcgtcgtcg gggtcagcgc gggcgcggcg gcggacgcag
     2041 gctcgggcgc gctggtcatc gcgcgcgggg tcggctcgcg gctcgtcgcc gactcgcaac
     2101 tgcacgcgct gcttcagtgg ctgctcttcg ggttcgtgct cgcgctcctg ctgctgctga
     2161 tcggcaggta tcaccgcgcg cgcgcgggcg actgacgggc gcacaacaat cgggcggtcg
     2221 gcccttgacc tcttacggcg tcggcgaagg tctggggctg gccgcccgtc gtttccgggt
     2281 tctcgaagtg aagactgttg cggcggaatc cgtggtcggg cgtgatgcgg gcggacgttc
     2341 gccgcgaagg cgcggacggc gcggaggtag cacggaggct ttcgaatttg gcatctcgca
     2401 tttcaaatct cctaccgcca gagcctccgt gcgccctccg cgtccaccgc gttcccgcgg
     2461 ctaatcctgc ttccccacac gcccaacttg aaggcgagcc gagtccgctc tctctcaccg
     2521 cgctcttgaa ggcggcgacg gcctgcgtcc tgctcctcct cttcttcgaa gcctccttcg
     2581 cgcaggacgc gcccgcgcac gcgcccgccg cgccgtcggc ggacgaactc gtcatcgacg
     2641 aagactacac cggcgacgtg ttcggcgtgg gccgcacggt gcgcgtgcgc gggcgcgtga
     2701 gccacggcgt catcgcgttc ggcggcgatg tcgtggtcga ggggcgcgtc gagggcgacg
     2761 tggcgacggt cggcggctcc gtctcgcaga gggaaggctc gtacgtcggc ggcgacgtga
     2821 ttatcctcgg cggcacgtac caccacggca agaccggccc cggtcgcaac ccgtcgagca
     2881 acacgatcat gttcgccggc tacgaggagg agctgcgcgg gctggcgcgc gacccttcgg
     2941 tgctgctgac gccggagttc acgctcgcct tcgtcggcct gcggctgctc ggggtgctgc
     3001 tgtggttcgt cgtctcgctc gtgctgacgg ccatctcgcc cggcgccgtc agccgcggcg
     3061 ccgcccgcct gcagctgacc tcgctgcgcg tggccgtcat cggcgtcctc ggcgcgctgg
     3121 tggcggggcc gggcgtcttc gcggcgctcc actacctgcc gcccgtgctc ggcgtgttcg
     3181 tcagcgcgac cgcgctgctc ctgctgctgt tcgcctacgt cttcgggcgc gtctgcatct
     3241 acgcggcgac cggcaggtgg ctccaacgac tcgtcctcgc cgaagacaag cgctcggaat
     3301 ccatcgcgct gctgctcggc gccgtcttct ggtcggtgct gctcacgctc ccctacgtgt
     3361 ggccgttcgt cttcgccggg ctgctcgtcg tctcgctcgg cctctcgctc acggcgcgtc
     3421 acccgctggg ccgcaagcgc gccgccgccg cggcggctta actcacgccc cgcgccgcgt
     3481 gcctgatata atccccgcga cccgcgccgc ccactcgatt cggacggcgc cgaccgccgg
     3541 tcgccgaaac ccgaaacccg acccgcagga gtcacacgcc cgatgaagac cctactcacc
     3601 gtcgcgttcg tctgcctcgc cgccgccgcc gtccgcgccc aacccgcgcc gcccgcgccg
     3661 aagccgatca cgccgccccc cgacccgcac gccgcgcaca aacaacagcc cccggctgag
     3721 gagccgacgg gcgcgcagaa gtggttcacc gatgtcgagc tgctgacgca ggacggcgcg
     3781 aaggtgcgct tctacagcga cgtgctcaag ggcaagacgg tcgtcgtcaa cgccttcttc
     3841 accacctgca cctctgtctg cccgccgatg aaccgcagca tggagaagat tcaggaggcg
     3901 ctcggcgacc gcgtcgggcg cgacgtcttc ctcgtttcca tcacggttga cccggtgacc
     3961 gacacgcccc cgcgcctcaa ggagtacgcg cggaagttcc acgcgcgcgc gggctggacg
     4021 ttcctcacgg ggaagaagga gaacgtggac tgggccttgt acaagctcgg ccagtacgtc
     4081 gagaacaagg acgaccacaa gaccgtcatc atcatcggca acgagacgac cggactctgg
     4141 aagaaagcct tcggcctggc gaaggccgaa gagctgatcc ggctcgtcga aggcgtggcg
     4201 aacgacaagg gcgccgcgcc gcccgtgaaa taaaacggac atgccccgcc cgcgacgccc
     4261 ctcgtcaagc acccgcggga gcctctgcca catcaaacta acccgaaccg aacaggcggt
     4321 agcgaacgaa ccacacaagg aaaatcagac ccggaggctt aatgaacagg ctcttattca
     4381 ctctcttcat cgccctcgtc tgcgccgcgc ccgccgccgc gcagacgcag gcgacgcagc
     4441 agcagccgcc gccgccgaag ccggccacgg tcgagacgtt gaacgcggcg gaggcggcca
     4501 tgctctgcgg ctgcgagggc gcgccgctgc ccgccgtgct cgccgtcgtc aacggcgtca
     4561 agatcacgcc cgccgacctc ggccccgaga cagccgagcg cgtgcgccag ctccagacgg
     4621 aagtcatcaa agcgcgcgag cgcgagctgg acttgcagat caactcgctg ctgctcgacg
     4681 ccgaggcgaa gaagcgcggc gtcaacacgt cgaaagtcat cgaggacgag atcgtctcca
     4741 aagcgaccga gccgaccgac gccgaggcgc aggcgttcta cgaccagaac aaggaacgga
     4801 ttcagaccgg cttcgcggac gtgaagccgc agatcgtctc ctacctgcgc gagcagcggc
     4861 agcgcgacct ggccgcgaag ctcgccgagc ggctgcgcgc cgccgccgcc gtcaagacca
     4921 acgtcgccac ggtgacgcag cccgccggcc cggcagatcg cgcgcgcgtc ttcgccaccg
     4981 tcaacggcag gaacatcact tcgggcgagg tggaggactc tctgcgctcg
//
LOCUS       DBNGOH010001155         1584 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2561584, whole genome shotgun sequence.
ACCESSION   DBNGOH010001155 DBNGOH010000000
VERSION     DBNGOH010001155.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1584)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1584)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1584
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2561584"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="AC159P_22420"
     CDS             <1..240
                     /locus_tag="AC159P_22420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3698255.1"
                     /translation="LPSVGGFNFADLRLVIYAALLIVIMLTRPQGVLGEREIGLHWLR
                     RAQKAPEGDKTVGASGGVPVAQVPGEKTAKEVENR"
     gene            237..1100
                     /locus_tag="AC159P_22425"
     CDS             237..1100
                     /locus_tag="AC159P_22425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017715321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3698256.1"
                     /translation="MSGAPEPSGVRATKEDSIVAEAARTARAKELWPDEDAILRTHKC
                     TIRFGGLVAVNELDMAVRPGEIYGLIGPNGAGKTTVFNLLTGVYKPTSGEILFQRHRI
                     DGLRPYQIARRGVSRTFQNIRLFAGMTVLENVMTACHRHAQQNLLDAVLRTPRFARDE
                     RDHRDFCLELLEIFDLARFRDEGGTSLAYGNQRRLEIVRALATQPQLLLLDEPAAGMN
                     PSEQDDLLNLIHQIRDRFGLTILLVEHNMKVVMGVCERIQVVDYGKSIALGLPEEIRN
                     DPKVIEAYLGD"
     gene            1185..>1584
                     /locus_tag="AC159P_22430"
     CDS             1185..>1584
                     /locus_tag="AC159P_22430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007290015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3698257.1"
                     /translation="MLTLENISVGYGAIRALKGVSMRVEQGEVVTLIGANGAGKTTTL
                     RTITGLLTPTEGRVTFEGEEIGGKPTHKLVARGISMSPEGRGVFANLTVRENLLMGAY
                     LKKNKAEIAEDLQRGFRMFPRLKERESQKAG"
BASE COUNT          294 a          541 c          521 g          228 t
ORIGIN      
        1 ctcccctcgg tcggcggctt caacttcgca gacctccggc tcgtcatcta cgccgcgctg
       61 ctcatcgtca tcatgctgac gaggccgcag ggcgtgctcg gcgagcgcga gatcggtttg
      121 cactggctgc gccgcgcgca gaaggcgccc gagggcgaca agaccgtggg cgcgtcgggc
      181 ggcgtgcccg tcgcgcaagt gccgggcgag aagacggcga aagaggtgga gaacagatga
      241 gcggcgcacc cgaaccgagc ggcgtcaggg cgaccaaaga ggactccatc gtcgcggagg
      301 ccgcgcgcac cgcgcgcgcg aaagagttgt ggccggacga ggacgccatt ctgcgcacgc
      361 acaagtgcac gatccgcttc ggcggcctcg tcgccgtcaa cgagctggac atggccgtgc
      421 ggcccggcga aatttacggc ctcatcggcc cgaacggcgc cggcaagacg accgtcttca
      481 acctgctgac cggcgtctac aagccgacct ccggcgaaat cctcttccag cgccaccgca
      541 tcgacggcct ccggccctac cagatcgcgc gccgcggcgt ctcgcgcacg ttccagaaca
      601 tacggctctt cgccggcatg accgtgctcg aaaacgtgat gaccgcctgc caccggcacg
      661 cgcagcagaa cctgctggac gcggtgctgc gcacgccgcg cttcgcgcgc gacgagcgcg
      721 accaccgcga cttctgtctc gaactgctgg aaatcttcga cctcgcgcgc ttccgcgacg
      781 agggcggcac cagcctcgcc tacggcaacc agcggcgcct ggagatcgtc cgcgcgctcg
      841 ccacgcagcc gcagctcctc ctcctggacg agccggcggc gggcatgaac ccctcggagc
      901 aggacgacct cctcaacctg atccatcaaa tccgcgaccg cttcggcctg accatcctgc
      961 tggtcgagca caacatgaaa gtcgtcatgg gcgtctgcga gcggatacag gtcgtggact
     1021 acggcaagtc catcgcgctc ggtctgcccg aagagatcag gaacgacccg aaggtgatcg
     1081 aagcttactt aggagattag cgggcagcgg gcggcgggca gcgggcagtt caagaaaact
     1141 gcttttgact gcctactgcc tactgcctac agcctactgc tctcatgctg acactcgaaa
     1201 acatttccgt gggctacggc gcgatccgcg cgctgaaggg cgtctcgatg cgggtcgagc
     1261 agggcgaggt ggtgacgctg atcggggcca acggggcggg gaagacgacg accctgcgca
     1321 cgatcacggg cctgctgacg ccgaccgagg ggcgcgtcac gttcgagggc gaggagatcg
     1381 gcggcaagcc cacgcacaag ctggtcgcgc gcggcatctc gatgtcgccc gagggccgcg
     1441 gcgtcttcgc caacctcacc gtccgcgaga acctcctgat gggcgcctat ctcaagaaga
     1501 acaaggccga gatagccgaa gacttgcagc gcggctttcg catgttcccg cgcctcaagg
     1561 agcgcgagtc gcagaaggcg ggga
//
LOCUS       DBNGOH010001156         4502 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2193061, whole genome shotgun sequence.
ACCESSION   DBNGOH010001156 DBNGOH010000000
VERSION     DBNGOH010001156.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4502)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4502)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4502
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2193061"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(210..563)
                     /locus_tag="AC159P_22435"
     CDS             complement(210..563)
                     /locus_tag="AC159P_22435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015182922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HIR3698258.1"
                     /translation="MLRLATDEDFNNRILRGLPRRRPGLDIVRVQDAGLMGEGDAAVL
                     EWAAGEGRVLLTHDVTTMMQYVDERIAAGLPMPGVFEVSQQTPIGQAIEDIFLLAECS
                     LEGEWEGQIRFLPLR"
     gene            complement(731..1048)
                     /locus_tag="AC159P_22440"
     CDS             complement(731..1048)
                     /locus_tag="AC159P_22440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017739755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HIR3698259.1"
                     /translation="MTEATTTVPLFTDEAGVLRVAGTRVSLDSVIHAFDQGATPEEIA
                     QDFPTLDLAAIYSVLGYYLQNRAEVEQYLEQRKVRRAESRKEIEARFNPHGLRERLLA
                     RKQ"
     gene            complement(1102..4329)
                     /locus_tag="AC159P_22445"
     CDS             complement(1102..4329)
                     /locus_tag="AC159P_22445"
                     /inference="COORDINATES: protein motif:HMM:NF012321.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HIR3698260.1"
                     /translation="MANGDEREGQRLDKELRERLRSVQSAAERRTIAGLARAVGKLPA
                     DVARAALEVSAGLAAGVSLRAGVEFLRAAPEAGRVLDAEELRAWGEMGRRLAMADAET
                     GAAFFAAGVGELEEVPRAARPPLFQVCARQLQLSTSVALETFRGAPELARAIQEAGLL
                     RAVYEVALEIARRSAKHSADFLAATPAAFAALRARGGNEAARAAYDAGAGGDDAAREA
                     VRVAESFAARAGGIAADMWQAVPAAVEGLSAEQVVALLGHAEGFLDRGGAAALNAFAA
                     GGEVLRLLPGAFAEWALLLRAVAEGSNAAMVALARTSPAFFRALAETGGQRARMDETA
                     RRVIVAAREVAHVDVEAALLCFRSGPLALRTASVEQFERWAADGLAPAESSARARRSY
                     YALETRRSNERLQTTGGGLALEDVAQTLRFYVEALTGRAVEVAPLAAVPDEQRIGDGR
                     TIHLPSTVAEFGDEALDFRLYKVLAAHAAGQIEFGTRARDTAELRAAYTSLADLYAPE
                     NVDALDAFALDGYINEPGKGERALSAEEEAREALKTQRALPADGDFRAALALFPQQGL
                     ASRIFGTLENGRIDRRLRHTYRGLARDLDLVRAHLGANRPRIAGLPVQLVPFELLFQI
                     TLCGGALDDARTFYAQIVSELETIVVDYLADARSTVADTLMATSRVYSLFQSVVPEMT
                     ERQPAEDEDAGADDESGAARANAEREKEERSDEAEGAQDHEQRQRDARELFNAWAAME
                     SPAGEGEETLTGAERWFAAESAEQELEPDEVAFDYDEWDRDLADTRVGWCRVVEKRVR
                     RGERSFVELTRSRYRGVISSIRHQFQLMRPENLKRVMNELDGEDYDLNALIDYVVDRR
                     ADGQQSERIYTKRLRRERDVAVSFLLDQSSSTARTIGRHPLQPYSHPGRRIIEIEKEG
                     LVLMSEALEAVGDPYSIYGFTSEGRRNVKFYVIKDFGERYAEEVEHRIGGINYQNNTR
                     LGAAVRHAAAKLSRQEARTRLLIVLSDGRPYDHDYGDARYAREDTREALRQARLDGIT
                     PFCITIDRDSEAELKDLYGEVGYTIIDDVLSLPERMPAIYRRLTT"
BASE COUNT          714 a         1585 c         1421 g          782 t
ORIGIN      
        1 aaggaggctc ctttctacga ctcgcagacc tgcgggggag aaacgttccg gctcgcctga
       61 gacttttgga gcgacgagag gacgcggcca actcccgctt tgtttcacgc cgtcagcctg
      121 cgatagatcg cgggcatccg ttcgggcaag ctgagcacgt cgtcgatgat ggtgtagccg
      181 acctcgccgt gggtgacgca catagcgctt tatcgtaacg gcaggaacct aatctggcct
      241 tcccattcac cctccaggct acattcggca agaagaaata tgtcttcgat agcttggccg
      301 atgggagttt gctggctgac ttcgaagact ccgggcatgg gcaatccggc ggcgatgcgc
      361 tcatcgacat attgcatcat cgtcgtcacg tcatgcgtga gcaaaacgcg tccttctccc
      421 gcagcccatt cgagcaccgc cgcatcccct tcgcccatga gtccggcatc ctgcacgcgc
      481 acgatgtcca gaccaggtct gcggcgcggc aacccgcgaa gaatacgatt gttgaaatct
      541 tcgtcggttg ctaagcgcag catcggcttc ttcttggcta ttgattaccc actaaaccgg
      601 aagattgccg gcgaccagca atcttcatga acggccatct tcatttatca ggtggagact
      661 gtaaaacaca gtaagggtta ctgttgccca ttatttcgaa gtcgtaaggg tggagtacga
      721 ccggcgcggg ttattgctta cgagccagga gacgttcgcg cagcccgtga gggttgaatc
      781 tcgcttcgat ctcttttctc gattccgcgc ggcgtacttt cctttgctca aggtactgtt
      841 ccacttcggc gcggttctgt aagtagtagc cgaggacgga atagatggcg gccagatcga
      901 gcgtcggaaa atcctgtgcg atttcctccg gcgtcgcgcc ctgatcgaag gcgtgtatga
      961 ccgagtcgag cgacacacgc gtgccggcga cacgtaagac gccggcctcg tccgtgaaca
     1021 gcggtactgt ggtcgtggct tcagtcatcg ccgaaaagta tagcctaaac ggccaatgct
     1081 taacaccgaa atcctttgcc tctacgtcgt cagccggcgg taaatcgcgg gcatgcgttc
     1141 gggcaaagat agcacgtcgt cgatgatggt gtagccgact tcgccgtaga ggtctttgag
     1201 ttcggcctcg gagtcgcggt cgatggtgat gcagaagggg gtgatgccgt cgaggcgggc
     1261 ttgtcgtaaa gcctcgcggg tgtcttcgcg ggcgtagcgg gcgtcgccgt agtcgtggtc
     1321 gtaggggcga ccgtcggaga ggacgatgag gaggcgggtg cgcgcctcct gccgggagag
     1381 cttggcggcg gcgtggcgga cggcggcgcc gaggcgcgtg ttgttctggt agttgatgcc
     1441 gccgatgcgg tgctcgacct cttcggcgta gcgctcgccg aaatctttga tgacgtaaaa
     1501 cttgacgttg cgtctgccct cagacgtgaa gccgtagatc gagtaggggt cgccgacggc
     1561 ttcgagcgcc tcgctcatca ggacgagtcc ttccttctcg atctcgatga tgcggcgacc
     1621 ggggtgcgag tagggctgca agggatggcg accgatggtg cgcgccgtcg aggaggactg
     1681 gtcgagcagg aacgagacgg ccacgtcgcg ctcgcgcctg aggcgcttcg tgtagatgcg
     1741 ctcggactgc tggccgtcgg cgcggcggtc aacgacgtag tcgatcagcg cgttcaggtc
     1801 gtagtcttcg ccgtccagct cgttcatcac gcgcttcagg ttctcggggc gcatcagttg
     1861 gaactggtgg cggatcgagg agatgacgcc gcggtagcgc gagcgcgtca gctcgacgaa
     1921 cgagcgctcg ccgcggcgga cgcgcttctc gacgacgcgg caccagccga cgcgcgtgtc
     1981 ggcgaggtct ctgtcccact cgtcgtagtc gaaggcgacc tcgtccggtt ccagctcctg
     2041 ctcggcggac tcggcggcga accagcgctc ggcgccggtc aaagtctcct cgccctcgcc
     2101 cgcgggcgat tccatcgcgg cccacgcgtt gaacagctcg cgcgcgtcgc gctgccgctg
     2161 ctcgtgatcc tgcgcgccct cggcctcgtc cgagcgctct tccttctcgc gctcggcgtt
     2221 ggcgcgcgcc gcgccgctct cgtcgtccgc gcccgcgtct tcgtcctcgg cgggctgccg
     2281 ctcggtcatc tcgggcacga ccgactggaa gagcgagtag acgcggctgg tcgccatcaa
     2341 ggtgtcggcg acggtcgagc gcgcgtcggc gaggtaatcg acgacgatgg tctccagctc
     2401 ggagacgatc tgcgcataga acgtgcgcgc gtcgtcgagc gcgccgccgc agagcgtgat
     2461 ctggaacagc agctcgaagg ggacgagctg gacgggcagc cccgcgatgc gcgggcggtt
     2521 cgcgccgagg tgcgcacgca cgaggtcgag gtcgcgcgcg agtccgcgat aagtatgtct
     2581 caaccggcgg tcgatgcgcc cgttctccag cgtgccgaag atgcgcgagg ctaacccctg
     2641 ctgcgggaag agcgcgagcg ccgcgcggaa gtcgccgtcc gccggcaaag ccctctgcgt
     2701 cttgagtgct tcgcgcgcct cttcttccgc cgacagcgcg cgctcgccct tgcccggctc
     2761 gttgatgtag ccgtcgagcg cgaaggcgtc gagcgcgtcc acgttctccg gcgcgtaaag
     2821 gtcggcgagc gacgtgtagg ccgcgcgcag ctccgccgtg tcgcgcgcgc gcgtgccgaa
     2881 ctcgatctgc cccgccgcgt gcgccgccag cactttgtag aggcggaagt ccagcgcctc
     2941 gtcgccgaac tcggcgaccg tcgaaggcag gtgaatcgtg cgcccgtcgc cgatgcgctg
     3001 ctcgtcgggc accgccgcga gcggcgccac ctcgaccgcg cgccccgtca aagcctcgac
     3061 gtagaagcgc aaggtctgcg ccacgtcttc gagcgcgagt ccgccgcccg tcgtctgcaa
     3121 gcgctcgttg ctccgccgcg tttcgagcgc gtaataactc cgccgcgcgc gcgcgctcga
     3181 ctccgccggc gcgaggccgt ctgctgccca ccgctcgaac tgctcgacgc tcgccgtgcg
     3241 caaagccagc ggccccgagc ggaagcacag cagcgccgct tcgacatcga cgtgcgccac
     3301 ctcgcgcgcc gccacgatga cgcgccgcgc cgtctcgtcc atgcgcgccc gttgcccccc
     3361 cgtctcggcg agcgcgcgga agaacgcggg gctggtgcgc gccagcgcca ccatcgcggc
     3421 gttgctccct tcggccaccg cgcgcagcag cagcgcccac tccgcgaacg cgcccggcag
     3481 gagcctgagc acctcgccgc ccgccgcgaa ggcgttgagc gccgccgctc cccctcgatc
     3541 aagaaacccc tcggcgtgac cgaggagcgc gaccacctgc tccgcgctca acccctcgac
     3601 cgccgccggc acggcctgcc acatgtccgc cgcgatgccg cccgcacgcg ccgcgaaact
     3661 ctcggccacg cgcacggcct cgcgcgccgc gtcgtcgcct ccggcgcccg cgtcataagc
     3721 ggcgcgcgcc gcttcgttcc ccccgcgcgc ccgcagcgcc gcgaacgcgg cgggcgtcgc
     3781 cgccagaaaa tccgcgctgt gcttcgccga gcggcgcgcg atctcaagcg cgacttcata
     3841 aaccgcgcgg agcagccccg cttcctgtat cgcgcgcgcc agctccggcg caccgcggaa
     3901 cgtctccaac gccaccgaag tggagagctg gagctggcgc gcgcacacct gaaacagagg
     3961 cggcctcgcc gcgcgcggca cctcctccaa ctcccccacg cccgccgcga agaacgccgc
     4021 gccagtctcc gcgtccgcca tcgccagacg cctccccatc tcgccccacg cgcgcaactc
     4081 ctccgcgtcg agcacgcgcc ccgcctccgg cgccgcacgc aaaaactcca cccccgcccg
     4141 caaactgacg cccgccgcca gccccgcgct cacctccaac gccgccctcg ccacgtccgc
     4201 cggcagcttc cccaccgccc gcgccagccc cgcaatcgtc cgtcgctccg ccgcgctttg
     4261 caccgaccgc agccgctcgc gcagctcctt atccaaccgc tggccttctc tctcatcgcc
     4321 gtttgccatc tacttaaatc aatcccgctg cctcgaatca ctcatttcta cgtacgcgcg
     4381 cagacgattg ccgaaaacct ttttgagctt gagtaagttt tcaactagcc atgcgtgtat
     4441 ttctcgaagc gttgccgcgt catcgtctat tgagccgggc cgactaacgc gaatactgca
     4501 ca
//
LOCUS       DBNGOH010001157         3844 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3843340, whole genome shotgun sequence.
ACCESSION   DBNGOH010001157 DBNGOH010000000
VERSION     DBNGOH010001157.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3844)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3844)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3844
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3843340"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1028)
                     /locus_tag="AC159P_22450"
     CDS             complement(<1..1028)
                     /locus_tag="AC159P_22450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018756395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR3698261.1"
                     /translation="MRTRRVHHFKQAAAALALFALLSASALQACGGGGGAPGDGQKVT
                     LTLAGYTTPREAYGKAIIPAFRKHWKEKTGQDVEFQESYQASGAQSRAIIGGFEADVV
                     ALSLEGDVDKITDAGLITHDWKAKPHRGMVSTSVVVIAVREGNPKGVKDWPDLARQGL
                     NVLTPDPKTSGGAQWNINAIYGAALRGHAGVAKDDPAAAREFLKSVFRNVSIMDKGAR
                     ESITNYEKGVGDVAITYENEVLVGRQGGQKYDYVIPRSTILIENPVALIDKNVDKHGT
                     RAVAEAFVNFLWEKEAQRAYAKYGLRPVDAEVAEEVKAQYPAVEDLWKVDYLGGWSRV
                     SREIYGPQG"
     gene            complement(1164..1616)
                     /locus_tag="AC159P_22455"
     CDS             complement(1164..1616)
                     /locus_tag="AC159P_22455"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RrF2 family transcriptional regulator"
                     /protein_id="HIR3698262.1"
                     /translation="MNVSTKSEYGLRALIYLAGEGEGRVVPAREIAEQWNVPIKYLEQ
                     ILKTLKEAGVVEGLVGVGGGYRLTRPATLITAGEVVRILDGRLAPMGCVSTYDYEPCE
                     FEPSCNLKTLWSRTRAAMLGVLDQTTVADLATPRSQQNVVNIKSACTR"
     gene            complement(1697..2965)
                     /locus_tag="AC159P_22460"
     CDS             complement(1697..2965)
                     /locus_tag="AC159P_22460"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3698263.1"
                     /translation="MRCPVCTSEIVEGEHCCAPRDTDSTGVPPTAISVKRLSKAFGGQ
                     RVLSEVSLDIAGEEMLVVLGPSGSGKTTLLRIIAGLEQPDAGDIYLRGRRANHLPPQA
                     RGLGVVFQEQALFQRMTVEQNIAFGLRLRRAAGERVRATVDEMIELTRLREHRRKTPA
                     QLSGGQRQRVAVARALAYRPDALLFDEPFSALDAVTRTRLRREVRVLLQTLSTPALFI
                     THDQEEALELGDRIAVINHGRVEQTDTPFEIYNHPRNEFVATFLGAANVLLGRWREGR
                     VRVGALDLKPPRSATPFAERQALKVVFRPEDVVLNFQPQLLGTPFYLGGGVVEEVSYT
                     GPVERLVVRLHFRPHRAEMPGGPARRPHLSPAEEAYTEGFPVTVMRTKWEAADMELSA
                     GDPVAVGLKDYRLLAHYPLDGETGAKALRR"
     gene            3282..3746
                     /locus_tag="AC159P_22465"
     CDS             3282..3746
                     /locus_tag="AC159P_22465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015930234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 family protein"
                     /protein_id="HIR3698264.1"
                     /translation="MTIYLLALLLGVVAGLRTMTAPAAVAWAAHLGWLDLGGTWLAFL
                     GSAWARWILTLLALVELVTDQLPSTPSRTVPVQFGARILSGALSGAAVGASGGSLVGG
                     LLAGIVGAVIGTLGGRAFRARLAAAFGSDRPAAFVEDAIAVGGALLIAVALP"
BASE COUNT          586 a         1282 c         1265 g          711 t
ORIGIN      
        1 ccctgcggcc cgtaaatctc cctcgagacc ctcgaccagc cgcccaggta atcgaccttc
       61 cacaggtctt ccactgccgg gtactgcgcc ttcacctcct cggccacctc cgcgtccacc
      121 ggcctcaagc cgtacttggc gtaggcgcgc tgcgcctcct tctcccacag gaagttgacg
      181 aacgcctcgg ccaccgcccg cgtcccgtgc ttgtccacgt tcttgtcgat cagcgccacc
      241 ggattctcga tcagaatcgt cgagcgcggg atcacgtagt cgtacttctg cccgccctgc
      301 cgcccgacga gcacttcgtt ctcgtaggtg atggccacgt cgcccacacc cttctcgtag
      361 ttcgtgatcg actcgcgcgc acccttgtcc atgatcgaga cgttgcggaa gacagacttc
      421 aggaactcgc gcgccgccgc tgggtcgtcc ttcgccaccc cggcgtggcc gcgcagcgcc
      481 gcgccgtaga tggcgttgat gttccactgc gcgccgcccg aggtcttcgg gtccggggtg
      541 agcacgttga ggccctgacg ggcgaggtcg ggccagtcct tgacgccctt cgggttgccc
      601 tcgcgcaccg cgatgacgac gaccgacgtg ctgaccatcc cgcggtgggg cttcgctttc
      661 cagtcgtggg tgatgaggcc ggcgtccgtg atcttgtcca cgtcgccttc cagagagagc
      721 gcgacgacgt cggcctcgaa gccgccgatg atggcgcgcg actgggcgcc gctggcctgg
      781 taggattctt ggaattcaac gtcctgcccg gtcttctcct tccagtgttt ccggaaggcg
      841 gggatgatgg ccttgccgta cgcctcgcgc ggcgtcgtgt agccggcgag cgtgagcgtc
      901 accttctggc cgtcgcccgg cgcgccgccg cccccgccgc acgcctgcaa cgcggacgcg
      961 gacagcagcg cgaagagcgc gagcgccgcc gcggcctgtt tgaaatgatg aactctgcgt
     1021 gtcctcatga attacctccg tcaactcgcg tcttcgattg cttgaaatgg atgaaccagt
     1081 caattaccta gtaaaatact aggagttgaa tataaatagc cgtgccccat tcacgccgcg
     1141 agcgggacgc gaaggggcac ggactagcgc gtacaggcgg acttgatgtt gacgacgttt
     1201 tgctgactgc ggggggtggc gaggtcagcg acggtggttt ggtcgagcac gccgagcatg
     1261 gcggcgcgcg tcctcgacca gagggttttc aggttgcagg agggttcgaa ctcacacggc
     1321 tcgtagtcgt acgtgctgac acagcccatc ggcgcgagcc tgccgtcgag tatgcggacg
     1381 acttccccgg cggtgatgag tgtcgcgggc cgcgtcagcc ggtagccgcc gccgacgcct
     1441 accagcccct cgacgacgcc cgcttctttg agggtcttga gaatctgttc gagatacttg
     1501 atcgggacgt tccactgctc cgcgatctcg cgcgcaggca ccacacgccc ctcgccctcc
     1561 ccggcaaggt agatcagcgc gcgaagcccg tattcgctct tcgtggagac gttcatcctt
     1621 aattcctagc taataggtag gagttaatac aacaagcggc gggagttcgt caacagtttt
     1681 tttctcgcgg cgagtcctaa cgcctgagtg ctttggctcc ggtctcgccg tcgagcgggt
     1741 agtgcgcgag gaggcggtag tctttcagcc cgacggcgac ggggtcgccc gccgacagct
     1801 ccatgtcggc ggcctcccac ttggtacgca tgacggtgac ggggaagcct tccgtgtaag
     1861 cttcttcggc gggcgagagg tgcggcctgc gcgcggggcc gcccggcatt tccgcgcggt
     1921 gcggtctgaa gtggaggcgg acgaccagcc gctcgacggg gcctgtgtag gagacttctt
     1981 cgaccacgcc gccgccgagg tagaagggcg tgccgagcaa ctgcggctgg aaattcagga
     2041 ccacgtcctc cgggcggaag acgaccttca gggcctggcg ctcggcgaaa ggggtcgcgc
     2101 tccgaggcgg cttgaggtcg agcgcgccga ccctcacgcg gccctcgcgc cagcggccca
     2161 acagcacgtt ggccgcgccg aggaaggtag cgacgaactc gttgcgcggg tggttgtaga
     2221 tttcgaacgg cgtgtccgtc tgctcgacgc gtccgtggtt gatgacggcg atgcggtcgc
     2281 cgagttcgag cgcctcctcc tggtcgtgcg tgatgaagag cgcgggcgtc gagagcgtct
     2341 ggagcagcac ccgcacctcg cgcctgagtc tcgtgcgcgt cacggcgtcg agcgcactga
     2401 acggctcgtc gaacaggagc gcgtccggcc tgtaggccag cgcgcgggcg acggcgacgc
     2461 gctgccgctg cccgcccgaa agctgtgccg gggtcttccg ccggtgctct cgcagccgtg
     2521 tcagctcaat catctcgtcc acggtcgcgc gcacgcgctc gcccgccgcg cgccgcaggc
     2581 gcagaccgaa ggcgatgttc tgttcgaccg tcatccgctg gaagagcgcc tgctcctgaa
     2641 agacgacgcc gaggccgcgc gcttgcggcg gcaggtggtt ggcgcggcga ccccgcaggt
     2701 aaatgtcgcc cgcgtccggc tgctccagcc cggcgataat ccgcaggagg gtcgtcttgc
     2761 cgctccccga agggccgagc acgacgagca tctcctcccc cgcgatgtcg agactgactt
     2821 cggacagtac ccgctgcccg ccgaacgcct tcgacagccg cttcacactg atggccgtcg
     2881 gcgggacccc ggtcgagtcc gtgtctcggg gtgcgcagca gtgctcgcct tcgacgatct
     2941 cgcttgtgca gacggggcag cgcatggata aaggattgta ccagttccgc aacaaggcac
     3001 cgccgccgcc cgccggggtc gttcgagacg gctgaccgcg ccggtcaaga gacacgtcat
     3061 ggtttctacc gctaccacgt tggtggtatc cgcgtcttgc actttactgt tagcaaaaaa
     3121 gacctcgatc ccgtgatgtc cgacgcgccc tcgaacgggc atagtgcggg gcgccggctt
     3181 tcaacaatca ggggcggcag gtcatgcgcg agccatttcg aagttcccgt gtgcgtcttt
     3241 aacgacgctg tcaaccgcac tgagagaagg atgaccacac catgactatc tatctgctcg
     3301 cgctcctgct cggcgtcgtg gcgggcttgc ggaccatgac cgccccggcg gctgtcgcct
     3361 gggcggcgca cctcggctgg ctcgacctcg gcgggacatg gctggccttt ctcggcagcg
     3421 cgtgggcacg ctggattctg acgctgctcg cgctggtcga gctggtcacg gaccaactcc
     3481 cgtccacgcc cagtcgcacg gtgccggttc agttcggcgc acgcatcctg tcgggcgcgc
     3541 tttccggcgc ggcggtcggg gcgtccggag ggtctctggt cggcggactg ctggcaggaa
     3601 tcgtcggcgc cgtcatcggc actctcgggg ggcgcgcctt ccgcgcgcgg ctcgctgcgg
     3661 cattcggcag cgatcgcccc gcggctttcg tcgaggacgc gattgccgtg ggcggagctc
     3721 tgctgatcgc ggtcgcattg ccatagacta accctgaccc cctcactgag gtgtctatcg
     3781 aaccgggcaa gacgtaagac taatctcgtc gagagagctt ttgtgttctg gaggcgacaa
     3841 ggac
//
LOCUS       DBNGOH010001158         2164 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3843404, whole genome shotgun sequence.
ACCESSION   DBNGOH010001158 DBNGOH010000000
VERSION     DBNGOH010001158.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2164)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2164)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2164
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3843404"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(161..976)
                     /locus_tag="AC159P_22470"
     CDS             complement(161..976)
                     /locus_tag="AC159P_22470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014098983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3698265.1"
                     /translation="MNRLAITGLVFIALLALVALSADALATHDVGATNLAARYLPPSS
                     EHWLGTDATGRDVFSRVIHGARISLRVGIVVVVVSAVVGTVIGAVAGYYGGWFDRIVS
                     GYVFNVFLAFPGLLLAIAMVAFLGAGLNKLILALCVIGWVGYARLIRGQVLKVREYDF
                     VQAARALGARDWRVLFVHIMPNAIQPLIVQASLGMAGAVLSEASLSFLGLGVPPPAPS
                     WGVMLDEARDLTTLRIAPHAMIVPGVFIALTVLAFNFLGDGLREYLDPRQRRR"
     gene            complement(1099..2091)
                     /locus_tag="AC159P_22475"
     CDS             complement(1099..2091)
                     /locus_tag="AC159P_22475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3698266.1"
                     /translation="MKRKLLDIARRLLILLLVVWTVVSLVTILIELVPGDPATAILGD
                     QATPEALAQFREKHGLDRPAFFFSFPADESGARSLRWHGMDNRYAEYWRSLLAGDMGN
                     SFRTNRPVRELILSRYGATIQLALAALVVAVAIAVPLGVVAGTNRGNLLDNALSVVAL
                     VGISLPSFVVGPLLIYVFAVWLGLLAPSGRFHWSSIILPAVTLGAALSALLTRMVRSS
                     VIEELGEDYVRTARAKGLNERTVVYKHVLKNGLIPVVTILGLQLGVLLAGAIITEKIF
                     NWPGLGLLLVDDGIAKRDYRLVQGCVLVISVTYILANTLTDMVYRWLDPRIRVS"
BASE COUNT          421 a          654 c          782 g          307 t
ORIGIN      
        1 agcgggatgg ggtaggtctt cgagagttca cgaatcgaga tggcgacgtg ctcgcggagg
       61 gcggcgcctt gagttgtcgg ggtcgaccct tcggtgaacg tgcgggcggt cattctggca
      121 atcaaacgcg gcgcgggcga gaaaggtcga tacggatgct tcagcggcgg cgctgccgcg
      181 ggtcgaggta ttcgcgcagg ccgtcgccga ggaagttgaa ggcgagcacg gtgagcgcga
      241 tgaagacgcc cggcacgatc atcgcgtgcg gcgcgatgcg cagggtcgtc aggtcgcgcg
      301 cctcgtcgag catcacgccc caggatggcg cgggcggcgg caccccgagg ccgaggaaag
      361 agagcgacgc ctcggagagc accgcgcccg ccatgccgag gctcgcctgc acgatgagcg
      421 gctggatcgc gttcggcatg atgtggacga agagcacgcg ccagtcgcgc gcgccgagtg
      481 cccgcgccgc ctgcacgaag tcgtactcgc gcaccttcaa gacctgcccg cgaatcagcc
      541 gcgcgtagcc gacccagccg atgacgcaca gcgcgaggat gagcttgttc agcccggcgc
      601 cgaggaaagc caccatcgca atcgccagca gcaggccggg gaaggcgagg aagacgttga
      661 agacgtagcc cgagacgatg cggtcgaacc agccgccgta gtagcccgca accgccccga
      721 tgacggtgcc gacgaccgcc gagacgacga ccaccacgat gccgacgcgc agagagatgc
      781 gcgcgccgtg gatgacgcgc gagaaaacgt cgcgcccggt cgcatccgtc cccagccagt
      841 gctcggaaga gggcggcaga taccgcgccg ccagattcgt cgcgcccacg tcgtgcgtcg
      901 ccagcgcgtc cgccgagagc gccaccagcg cgagcagggc gatgaagacg aggccggtga
      961 tggctagacg attcatagcg attgcggatt gcggattgcg gattgcggat tggcgtgttt
     1021 cataaacctg agcggaatct ttagaggttc aactgtcatc actcgattaa tccaaaatcc
     1081 gcaatccgca ttccgcaatc aagagactct gattcgcggg tcgagccagc ggtagaccat
     1141 gtccgtgagc gtgttcgcca ggatgtaggt gacgctgatg acgaggacgc agccctgcac
     1201 gaggcggtag tcgcgcttgg cgatgccgtc gtccacgagg agcagcccga ggccgggcca
     1261 gttgaaaatc ttctcggtga tgatcgcgcc cgccagcagc acgccgagtt gcaggccgag
     1321 aatcgtgacg accgggatga gtccgttctt caggacgtgt ttgtagacga ccgtgcgctc
     1381 gttgaggccc ttggcgcgcg cggtgcggac gtagtcttcg ccgagttctt cgatgaccga
     1441 ggagcgcacc atgcgcgtga gcagcgcgga gagggccgcg ccgagcgtga cggcgggcag
     1501 gatgatgctc gaccagtgga agcggcccga gggggcgagc aggccgagcc agacggcgaa
     1561 gacgtagatc aggagcgggc cgacgacgaa gctcggcagg ctgatgccga cgagcgcgac
     1621 gaccgagagc gcgttgtcca agaggttccc gcggttcgtc cccgcgacga cgccgagcgg
     1681 gacggcgatg gcgaccgcga cgacgagcgc cgcgagcgcc aactgaatcg tcgccccgta
     1741 gcgcgagagg atcagctcgc gcacgggccg gttggtgcgg aacgagttgc ccatgtcgcc
     1801 cgcgagcagc gagcgccagt attcggcgta gcggttgtcc atgccgtgcc agcgcaggct
     1861 gcgcgcgccg ctctcgtccg cggggaagga gaagaagaag gccgggcggt cgagcccgtg
     1921 cttctctcgg aactgcgcga gcgcctcggg cgtcgcctgg tcgccgagga tggcggtcgc
     1981 cgggtcgccc ggcaccagct caatcagaat cgtcaccagg ctcacgaccg tccacacgac
     2041 gagcagcagg atgagcagac ggcgcgcgat gtcgagcagc ttgcgcttca tcaaaattaa
     2101 ctttaaaggg aattcggatt gcggagagat aatagcattg agcgcggtga agcatgaagg
     2161 ggtg
//
LOCUS       DBNGOH010001159         4728 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3477810, whole genome shotgun sequence.
ACCESSION   DBNGOH010001159 DBNGOH010000000
VERSION     DBNGOH010001159.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4728)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4728)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4728
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3477810"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..443)
                     /locus_tag="AC159P_22480"
     CDS             complement(<1..443)
                     /locus_tag="AC159P_22480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HIR3698267.1"
                     /translation="MNPAEATREIYWNVSHVWVMYALLVPTALVAGYGVYRHLSRWRR
                     GQPAARFDRPAARVRLLLRHAVAQRRTARELYAGLFHGFISYGFVVLTMATTVVALDA
                     DFGTSIMRGNFYLYFQSFTVDLFGALVMVGVVMAAARRYLKRPRKL"
     gene            complement(515..1495)
                     /locus_tag="AC159P_22485"
     CDS             complement(515..1495)
                     /locus_tag="AC159P_22485"
                     /inference="COORDINATES: protein motif:HMM:NF013200.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HIR3698268.1"
                     /translation="MNDVLICLFAAAGFDKGAQGLAGAGRRLADESGGRLRAVVLGAG
                     AETLAREVAHVADAVTVADQAELAEYQPETCLTALAQLCGEPRPRAVLLANDTYSQEI
                     APRLAHRLGGSAVGDCVEVRAAEGGAVRVSRQVYGGKAQAVIALKRSPAVVWLRARAF
                     APAASRAGACEVTRAALDIEADTRMRVVERRREEGGESRLEEARVIVSGGRGIGGPEG
                     FEAELRPLAELLGAQMAASRAACDAGWVPPTWQVGQTGKKVTPELYLAIAITGASQHM
                     AGISEAKTIAAINTDPDAPIFKHCRFGIVEDYRKVVPLLREKLKKAANGK"
     gene            complement(1600..2406)
                     /locus_tag="AC159P_22490"
     CDS             complement(1600..2406)
                     /locus_tag="AC159P_22490"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HIR3698269.1"
                     /translation="MNILVCLKQILDPEIPARDFRVDPVRREAERGGASLVTNIFCEN
                     ALETALQLRERAGGAHITVLSYGQPTAEDTLRKALAMKADAAALVLNESPTTPDPLAV
                     ARVLAAAIRRLGAFDLVMVGREAGDWGAGQTGGLLAEELGLPCVSFVDHIETAGGGLR
                     LKRQTDTGWEVLEATPPVVVTVTNDEHNVPRIPKVRDVMMSYRQPLTRFTHEDLGFTT
                     DEARAANTYYEVAELFIPQKQTRCEFVAGDTLDEKVEAFARRIVEVTRAI"
     gene            complement(2490..3149)
                     /locus_tag="AC159P_22495"
     CDS             complement(2490..3149)
                     /locus_tag="AC159P_22495"
                     /inference="COORDINATES: protein motif:HMM:NF036525.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR3698270.1"
                     /translation="MKAQKKSAPNNERLAEIYRAAAQIILRKGYDATSVNDIANALGM
                     TKAGLYHYINGKKELLYDIMNFGLGELDEEVAAPAKAIADPSARLRFIVASHARLVTR
                     GQGAITILVDEITALTPAQHRKITQRKRAYFDYLRDTLDELKAAGKLQDVDTTAATFS
                     LLGIVNWLSRWFRQGGALTEEQAAEQIVKIALHGLLRPEPRNARLKAVSSRQQAADST
                     K"
     gene            3313..>4728
                     /locus_tag="AC159P_22500"
     CDS             3313..>4728
                     /locus_tag="AC159P_22500"
                     /inference="COORDINATES: protein motif:HMM:NF012656.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR3698271.1"
                     /translation="MASQADVLESGAGAGFLTAEAAPGEVFTREDLSPAQQMFGRIAE
                     DFMRAEVLPRDAEIHAKDWALTRELLLKAGELDLLRVDIPEAYGGLGLDKVSSAYVGE
                     KIGVMPSFAGSLGAHAGIGTLPLVYFGNEEQKARYLPRLASGEWVAAYALTEPGSGSD
                     ARAARTKATLTEDGETYVLNGQKMWITNGGFADVFTIFAQVGGDRFTAFVVERGMGVV
                     SGREEPKLGLDGSSTTALILDNVRVPAANVLGRVGEGHRVAFNILNLGRLKLGTRNIG
                     GARQALTRAARYAVERQQFGRAICEFGMIKQKLGEMAVRCYVGDAMVYRTLGDVDRAL
                     AAVPAEDSERVLKVIESFAVECSINKVWTSEALAFVVDEALQTYGGYGYSKEYPAERA
                     YRDARITRIYEGTNEINRLIIPTRLLKNAAAARLFDGDHARRALEEVDDETARPSSST
                     LASERDLLTRAKRLVVFTLGLA"
BASE COUNT          736 a         1580 c         1603 g          809 t
ORIGIN      
        1 agtttccgcg ggcgcttcag ataacgacgc gcggcggcca tcacgacgcc gaccatgacg
       61 agcgccccga acagatcgac cgtgaaggat tggaagtaga gatagaagtt gccgcgcatg
      121 atgctcgtgc cgaagtcggc gtcgagcgcg acgaccgtcg tcgccatcgt gaggacgacg
      181 aagccgtagc tgatgaaccc gtgaaacagc cccgcgtaca gttcgcgcgc cgtgcgccgc
      241 tgcgcgaccg cgtgcctgag caacagcctg acgcgcgcgg cggggcggtc gaagcgcgcg
      301 gcgggctgcc cgcgccgcca acggctcaag tgccgataca ctccgtagcc cgccaccaac
      361 gctgtcggca cgagcagcgc gtacatcacc cacacgtggg agacgttcca gtaaatctcg
      421 cgcgtggctt cggcaggatt catctttaca aggacagtga atagtagatg gtgaatggtg
      481 aatggaaaat cggctgttat ctattcacca tttactattt accattcgct gcttttttca
      541 gcttttcgcg cagcagcggc acgaccttgc gatagtcctc gacgatgccg aagcggcagt
      601 gtttgaagat gggcgcgtcg gggtcggtgt tgatggcggc gatggtcttg gcctccgaga
      661 tgccggccat gtgctggctc gcgccggtga tggctatggc caggtatagc tccggcgtca
      721 ccttcttgcc ggtctggccg acctgccagg tgggcggcac ccagcccgcg tcgcacgccg
      781 cgcgcgaggc ggccatctgc gccccgagca actccgcgag cggcctcaac tcggcctcga
      841 agccttcggg gccgccaatg ccgcgcccgc ccgagacgat cacgcgcgcc tcttcgagcc
      901 gcgactcgcc gccctcttcg cgcctgcgct cgacgacgcg catgcgcgtg tcggcttcga
      961 tgtcgagcgc ggcgcgcgtc acctcgcaag ccccggcgcg cgacgccgcg ggcgcgaacg
     1021 cgcgcgcgcg cagccacacg accgcggggc ttcgtttcaa cgcgatcacc gcctgcgcct
     1081 tgccgccgta gacctgacgg ctgacgcgca ccgcaccgcc ctccgcggcg cgcacttcca
     1141 cgcagtcgcc gaccgcgctc cccccaagcc tgtgcgcgag gcgcggcgcg atctcctgac
     1201 tgtacgtgtc gttcgcaagc agcacggcgc gcggtcgcgg ctctccgcaa agctgcgcga
     1261 gcgccgtcag gcaggtctcc ggctggtact cggccagctc cgcctgatcc gcgaccgtca
     1321 ccgcgtcggc gacgtgcgcg acctcccgcg cgagcgtctc cgcgccggcg cccaagacga
     1381 ccgcgcgcaa acgcccgccc gactcgtccg cgagacgacg gcccgcgccc gcaagcccct
     1441 gcgcgccctt gtcgaaaccg gcggcggcga aaagacagat gagaacgtcg ttcatatacg
     1501 gagtttcgga acaagggttt tgggtgttgg gttctgggtg ttgggttaaa gacaattcgc
     1561 ttttaccaac acccagaacc caacacccaa aaccctcttt catattgccc gcgtcacctc
     1621 gacgatgcgg cgggcgaagg cttcgacttt ctcgtcgagc gtgtcgcccg cgacgaactc
     1681 gcagcgcgtc tgcttctgcg ggatgaacag ctctgccact tcgtagtagg tgttcgcggc
     1741 gcgcgcctcg tccgtagtga agccgaggtc ttcgtgcgtg aacctggtga gcggctggcg
     1801 gtacgacatc atcacgtcgc gcaccttcgg gatgcgcggg acgttgtgct cgtcgttggt
     1861 cacggtgacg acgacgggcg gcgtcgcttc gagcacttcc cagccggtgt ccgtctgccg
     1921 cttgagacgc agcccgccgc ccgccgtctc gatgtgatcg acgaaggaga cgcagggcag
     1981 tcccagctct tccgcgagca gcccgcccgt ctgccctgcg ccccagtccc ccgcctcgcg
     2041 cccgaccatg acgaggtcga acgcgccgag cctgcggatg gccgccgcca gcacgcgcgc
     2101 cacggcgagc gggtcgggcg tcgtggggct ttcattcagg acgagcgccg cggcgtcggc
     2161 cttcatcgcc agcgccttgc gcagagtgtc ttccgccgtc ggctggccgt acgagagcac
     2221 cgtgatgtgc gcgccgcccg cgcgctcgcg gagttgcagg gcggtttcga gcgcgttctc
     2281 gcagaagatg ttcgtcacga ggctcgcccc gccccgctcg gcttcgcgcc gcacgggatc
     2341 gacgcgaaag tcgcgcgccg ggatttccgg gtcgagaatc tgcttgagac agacgaggat
     2401 gttcatagaa taaagtaggc agtaggcagc aggcagtagg cagtcaagac aaaacagatt
     2461 gccgccggcc tgcggttctc atgcgcccct tatttcgtac tgtctgctgc ctgctgcctg
     2521 ctgcttactg ctttcagtct cgcgtttctc ggttcggggc gcagcaggcc gtggagggcg
     2581 attttgacga tctgttccgc cgcttgctcc tctgtgagtg cgccgccctg acggaaccag
     2641 cgcgagagcc agttgacgat gccgaggagg ctgaaggtcg cggcggtcgt gtccacgtct
     2701 tgcagcttcc ccgccgcctt cagctcgtcg agcgtgtcgc gcaggtagtc gaagtaggcg
     2761 cgcttgcgtt gggtgatctt ccggtgctgc gcgggcgtga gcgccgtgat ctcgtccacg
     2821 aggatggtga tggcgccctg cccgcgcgtg acgagccgcg cgtggctggc gacgatgaag
     2881 cgcaggcgcg cggacgggtc ggcgatggcc ttcgcgggcg ccgccacctc ctcgtccaat
     2941 tcgcccagcc cgaagttcat gatgtcgtag agcagctctt tcttgccgtt gatgtaatgg
     3001 tagaggcccg ccttggtcat cccgagggcg ttggcgatgt cgttgaccga ggtcgcgtcg
     3061 tagcccttgc ggaggatgat ctgcgccgcg gcgcggtata tttccgccag gcgctcgttg
     3121 ttgggggccg attttttctg tgctttcatc agtgccgttg caggtcgcgt ggggtcgccg
     3181 ggccggagct tgtgcgcgaa aggccgcggt gctatgttac cgaccgtccg gtcggtagtt
     3241 ataaccccga accgcgccca gcgtcaacac cgtcgcgcgg gcggcgtgcg ggaaacgaaa
     3301 gagggcgctt tcatggcatc acaagccgac gtgctcgaat cgggcgcggg cgccggcttc
     3361 ctgacggcgg aggccgcgcc gggagaggtc ttcacgcgcg aagacctctc gcccgcgcag
     3421 caaatgttcg gtcgcatcgc ggaggatttc atgcgcgcgg aggtgttgcc gcgcgacgcc
     3481 gagattcacg cgaaggactg ggccttgacg cgcgagctgc tgctgaaggc gggcgagctg
     3541 gacttgctgc gcgtggacat ccccgaggcg tacggcgggc tgggactgga caaggtcagc
     3601 tcggcctacg tgggcgagaa gatcggcgtc atgccttcgt tcgccgggtc gctcggcgcg
     3661 cacgcaggca tcgggacgct gccgctggtc tacttcggca acgaggagca gaaggcgcgc
     3721 tacctgccgc gcctcgcgag cggcgagtgg gtcgccgctt acgcgctgac cgaacccggc
     3781 tccggctcgg acgcgcgtgc ggcgcgcacg aaggcgacgc tgacggagga cggcgagacg
     3841 tacgtcctca acggccagaa gatgtggatc acgaacggcg gcttcgccga cgtgttcacc
     3901 atcttcgcgc aggtcggcgg cgacaggttc acggccttcg tcgtcgagcg cgggatgggt
     3961 gtcgtgagcg ggcgcgagga gccgaagctc ggcctcgacg gctcttccac gacggccctc
     4021 atcctcgaca acgtgcgcgt gcccgccgcg aacgtgctcg ggcgcgtcgg cgagggccac
     4081 cgcgtcgcct tcaacatcct gaacctcggt cggctcaagc tcggcacgcg caacatcggc
     4141 ggcgcgcggc aggctttgac gcgcgcggcc cgctacgcgg tcgagcggca gcagttcggg
     4201 cgcgccatct gcgagttcgg gatgatcaag cagaagctgg gcgagatggc ggtgcgctgc
     4261 tacgtcggcg acgcgatggt ctaccgcacg ctcggcgacg tggatcgcgc gctcgcagcc
     4321 gtccccgcgg aagactcgga gcgcgtgttg aaggtgatcg agagcttcgc cgtcgagtgc
     4381 tccatcaaca aggtctggac gagcgaggcg ctggccttcg tcgtcgatga ggcgttgcag
     4441 acctacggcg gctacggcta ctcgaaagag tacccggcgg agcgcgccta ccgcgacgcg
     4501 cgcatcacgc gcatctacga gggcacgaac gagatcaacc gcctcatcat cccgacgcgc
     4561 cttttgaaga acgccgccgc cgcgcgcctc ttcgacggcg accacgcgcg ccgcgccctc
     4621 gaagaggttg acgacgagac ggcgcgacct tcatcctcca cgctcgcgtc ggagcgcgac
     4681 cttttgacga gagccaaacg tctcgtcgtc ttcacgctcg ggctggcg
//
LOCUS       DBNGOH010001160         1678 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_178412, whole genome shotgun sequence.
ACCESSION   DBNGOH010001160 DBNGOH010000000
VERSION     DBNGOH010001160.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1678)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1678)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1678
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_178412"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(256..1563)
                     /locus_tag="AC159P_22505"
     CDS             complement(256..1563)
                     /locus_tag="AC159P_22505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="beta/alpha barrel domain-containing protein"
                     /protein_id="HIR3698272.1"
                     /translation="MLLQDLIRGVDLSAPLASSITDDYERLADGLPPRLEEYVLERYR
                     LDLTSDYGGLPVKNPFGKASGQLSLNVRQVSRDAAAGLGFVVLKTIIAEDQRGGQSMS
                     EWAIPETRMLVERIAGADGAEGWNVTWKGRGWYDTFDAYLDFFSESLAVGDESGMLVV
                     PSCKYHLPRPEEGEWRVGEYEHTTRRLLDVWDERRRGGGVMPLEKDFSPTLAGSDRAA
                     QVDTIRRWMSEVTRLIREAAAARPVSVGLKLFNAVFEDGFQIEMLRLINEECGVGGRP
                     DFIIYANRLFDPEREFEGRRGVAYGGPDLSARNLRVLARLRALEQRGEIPRCDFPISA
                     TGDIASGSMAAEYLLRGASTFQMHTFFQLPSSEYRMRGGSKTEQALHELYFNPDRGLI
                     ACLLRLRSALDWPADWNIKRMAEYCADPANGLWAATEPSLIRA"
BASE COUNT          252 a          585 c          563 g          278 t
ORIGIN      
        1 cggttcccgc ccttcagaaa gtcgttgccg ccgcgcatgt agcgcatgaa gtaggcgccg
       61 acgcccgcca tcaggcacag gtatccgacc agcactacgt agtctatggc gtgtatcgct
      121 gaacgcatac cagttccctc acgagcgtca ccggctgcgt ccgggcgccg agatgccagt
      181 catattggaa gcggcttgta aaatttctcg acgggcggcg cgccgcccgc ggggtcgcgg
      241 gccgcgcggc gcgccttacg cccggatcaa agacggctcg gtcgcggccc acaggccgtt
      301 cgccgggtcg gcgcagtatt cggccatgcg cttgatgttc cagtccgccg gccagtcgag
      361 cgcgctcctc aggcgcagca ggcaagcgat aagcccgcga tccgggttga aataaagctc
      421 gtgcagcgcc tgctcggtct tgctgccgcc gcgcatccgg tactcgctgc tcggcagttg
      481 gaagaacgtg tgcatctgga acgtgctcgc cccccgcagc aggtactcgg cggccatgct
      541 ccccgacgcg atgtcgcccg tcgcgctgat cgggaagtcg caccgtggta tctcgccgcg
      601 ctgctccagc gcgcgcagcc gcgcgagcac gcgcaggttg cgtgcgctca ggtcggggcc
      661 gccgtaggcg acaccccgcc ggccctcgaa ttcacgctcg gggtcgaaga gccggttggc
      721 gtagatgatg aagtcgggcc gacctcccac gccgcattcc tcgttgatga ggcgcagcat
      781 ctctatctgg aagccgtcct cgaagacggc gttgaagagc ttgaggccga cgctgacggg
      841 gcgcgcggcg gcggcttcgc ggatcagccg cgtcacctcg gacatccagc gcctgatggt
      901 gtccacctgc gcggcgcggt cggagccggc gagcgtcggg ctgaagtcct tttcgagcgg
      961 catcacgccg ccgccccgcc gccgctcgtc ccacacgtcg aggagccgcc gcgtcgtgtg
     1021 ttcgtattcg ccgacccgcc actcgccctc ctcgggccgc ggcaggtgat acttgcagga
     1081 gggcacgacg agcatccccg attcgtcgcc gaccgcgagc gactccgaga agaagtcgag
     1141 gtaggcgtcg aaggtgtcgt accagccgcg acccttccag gtcacgttcc agccctcggc
     1201 gccgtccgcg cccgcgatgc gctcgacgag catccgcgtc tcggggatgg cccactcgct
     1261 catcgactgc ccgccccgct gatcctcggc gatgatggtt ttgaggacga cgaagccaag
     1321 cccggcggcg gcgtcgcggc tcacctgccg gacgttcagc gagagttgcc ccgaagcctt
     1381 cccgaagggg ttcttgacgg ggaggccgcc gtagtcgctc gtgaggtcga ggcggtaacg
     1441 ttcgagcacg tactcttcga gccgcggcgg gaggccgtcg gcgagccgct cgtagtcgtc
     1501 cgtgattgat gaggcgaggg gcgcgctgag atcgacgccc cgaatcaagt cctgtagcag
     1561 catcagtccc caactcctcg gagggcggcg agcatgcgtc ccacttcttc gtactcgctc
     1621 cgcggcagcc tcggccccca cgggtcgtgg gcggcctcgg cgggtatcac gccctgat
//
LOCUS       DBNGOH010001161         5896 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4209153, whole genome shotgun sequence.
ACCESSION   DBNGOH010001161 DBNGOH010000000
VERSION     DBNGOH010001161.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5896)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5896)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5896
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4209153"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1061)
                     /locus_tag="AC159P_22510"
     CDS             complement(<1..1061)
                     /locus_tag="AC159P_22510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6298 domain-containing protein"
                     /protein_id="HIR3698273.1"
                     /translation="MNQYRLIRYGTLFLICVLLEAGHAQTRPAISVKLGADGRLVYES
                     DAQGNRVVDFSHAGYGGGGLAIPSVSAKLYVEPGGGNDRQRIQAALDLVSAMPLDADG
                     FRGAVLLKKGRYDIDSGIRINASGVVLRGEGNGEDGTVLVANGTSRRSLIVVEGAGER
                     TKIEDSRRTVADEYVPAGAMTLTLDRADGLKAGDRIVVHRPSTVEWISLLGMNTFPGW
                     RPESRLHWQPGSRDIEWDRTVTAVEGNRITVDAPLTTALDKKYGGGSVYRYEFPGRIS
                     RVGIENLRCVSAYDSARTWDEDHAWFAVSLDKVENAWVRQLTALHFVSYVVNAEADAI
                     RLTVEDCEALDPVSEIGGYR"
     gene            complement(1213..2589)
                     /locus_tag="AC159P_22515"
     CDS             complement(1213..2589)
                     /locus_tag="AC159P_22515"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010543375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016837 - carbon-oxygen lyase activity,
                     acting on polysaccharides [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectate lyase family protein"
                     /protein_id="HIR3698274.1"
                     /translation="MKAVSGLVLCLAGLLLTLLPPPARVASAPTERPAVPAFPGAEGF
                     GSTTPGGRGGRVILVTNLEDSGPGSLRAACEAEGPRVVVFRVAGLITLASPIVVKNPY
                     LTIAGQTAPGDGVCLRNHTFIVATHDVIVRYLRSRLGDLVGEAADAITLGTGARNVVL
                     DHCSASWSIDEVLSLTGNVSDVTVQWCLIAEALNRSKHQKGEHGYGSLSRANGPVSWH
                     HNLWAHNDARNPRLGDNNGRPPYPTFDVRNNVVYDYGDIASGLTQGVLKVNYVANYIR
                     PGPSSKARTPIRVGGPSELLFYISGNVFEGDAKLTADNSLFFDPVMIDGKRQVQTVAA
                     PFAAQPVRTLPAAEAYEKVLAEVGATLPVRDAVDTRIVDEVRQRKGSIIDSQKQVGGW
                     PEYRRAAPPKDSDADGMPDEWEAARRLDPRDPADASRDRDGDGYTNVEEYINDLTSHT
                     KPARRAPR"
     gene            complement(2616..>5896)
                     /locus_tag="AC159P_22520"
     CDS             complement(2616..>5896)
                     /locus_tag="AC159P_22520"
                     /inference="COORDINATES: protein motif:HMM:NF019311.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PA14 domain-containing protein"
                     /protein_id="HIR3698275.1"
                     /translation="RLWHDKQAAWQQDELFFPAPDISKLPVNPPTGGWEVLNADRGRE
                     VSVPGTVEEYLQEVAGPEGDLTGVSWWYRTVVVPAASSPRRVLLRFESVRLRAEVYVD
                     GKLVGYDLVGNTPFEVDLSGVARPGATVRLAVRVTDPGGNFDWRDGNTIKWGRVQVPG
                     SHGFGGITGRVELLSCDPAYVADIYVQNTPAITEVNAEVSVRNTAAAEVRRDLEVRVV
                     KHRDPSAVIFRKLLKGVTLKPGANLIPLKVSAPKADLWDTEHPNLYVFEASLKNGNRA
                     TDADRQTFGFRWFEPTGIGQDAVFRLNGKRVVLRTAISWGYWPVNGIFPTPGLAEQEI
                     RAAKQMGLNMLNFHRAIGHAVVLDKADELGLLYYEEPGNYRSGGSDPFVQALVREKLL
                     RMVRRDRSHPSLVVYNMINEMGQTTEAVVKKHEQDMRDAHALDPSRAITRTSAWATAK
                     DVEDPFKMHMRPFDERVYLSGWYDYHHAGGPAVWSQNLYRNPREYYNLTDNAREIVFW
                     GEEGAISTPPRLEKIKEALAASPNKGWDGQMYLDWYKAFDDFLTRKKLRTVFPTVDAL
                     TAEMGAVSLYHQGRKIENIRISNTSDGYAINGWEAQIIENHSGVVDVFRNPKSDPALV
                     AHYNQPLYVAVKVRTQVVQAPARVAVDFYAVNEKNLKGPHTLKVSARDTAGKVVFDKE
                     MPVTLAGGDTYGQLLAEGLEVPFAEGAGGMFRVEAELVSRAGRVEARGHDEVFVVDWK
                     GANLRGKGAVWEVGSHVRGFLENLKKLDAPAYKDDLGRLDWVVVSQPPSGAGFRSIPT
                     EQLSDPTGKPGLLATFFLGQDFAQRVHQRVDKTLSFSVSEGETPDPSVPTTENYGVRW
                     EGQLTPSASGRHVFSIQTSGNVRLTVNGRVLVDAMTTRGGPDKRGEIDLEAGRPVPVL
                     VEYRQQRGAGRIKLLWSYPVSDAPDPQRLIERARRDGTTVMIVDYAEDWMGLVGKNAP
                     VTYGGAFKVGVTWLGGVHFVRPHALVKGLPAGGAMNWPYQAVVRNGNERAGLLLEGEE
                     LVVGAWHSFPMQLGTAVGVIQVGEGRIVVSTLDLVENLPSEEGPAQVARKLLCNFIEF
                     AATT"
BASE COUNT          922 a         1920 c         1992 g         1062 t
ORIGIN      
        1 cggtagccgc cgatctccga cacggggtcg agcgcctcgc agtcttcgac cgtgagcctg
       61 atggcgtcgg cctctgcgtt gacgacgtag ctcacgaagt ggagggccgt caactgccgc
      121 acccacgcgt tctcgacctt gtccagactc acggcgaacc aagcgtgatc ctcgtcccac
      181 gttcgcgcgg agtcgtatgc ggagacgcac cgcaggttct ctatcccgac gcggctgatg
      241 cggcccggaa attcgtagcg gtagaccgag ccgccgccgt atttcttatc aagcgccgtg
      301 gtcagcggcg cgtcaaccgt gatgcgattc ccttcgaccg cggtgacggt gcgatcccat
      361 tcgatgtcgc gcgagccggg ttgccagtgg agacggcttt cgggacgcca gccggggaag
      421 gtgttcatgc cgagcagcga aatccactcg acagtggacg ggcggtgcac gacgattctg
      481 tccccggcct tcaacccgtc cgccctgtcg agtgtcaaag tcatcgcgcc cgccggcacg
      541 tattcgtcgg cgaccgttct tctcgaatct tcgatctttg tccgctcgcc cgcgccctcg
      601 accacgatca acgaacggcg cgaagtgccg ttggcgacga gaaccgtccc gtcctcgccg
      661 ttgccctcgc cgcggagcac gacaccgctc gcgttaatcc tgatgcccga atcgatgtcg
      721 tatctcccct tcttcaacag gacggcgccc cgaaacccgt ccgcgtcgag cggcatggcc
      781 gagacgaggt caagcgcggc ctgtatgcgt tgacggtcgt tgccgccgcc cggctcgacg
      841 taaagcttcg ccgacacaga ggggatagcc agccccccgc ccccgtaccc tgcgtgagag
      901 aaatcgacga cgcggttgcc ctgcgcgtcc gactcgtaga cgagcctgcc gtccgcgccg
      961 agcttcacgg agatggccgg tctggtctga gcgtgtcccg cttcgagcag gacgcagatc
     1021 agaaataatg tcccgtacct aatcaagcgg tattgattca tatcaatgtg cagttgagtg
     1081 tgaccgacgg gcggttcaac gcagagtgca cagagagact cggagggcgc agaggaagaa
     1141 atttcttctc tgcgccctcc gcgctcctcc gcgcgctctg cgttgaattc cgcctcccgc
     1201 tgcacgcaac gtttatcgag gcgcgcgacg ggcaggtttg gtgtgcgaag tcagatcgtt
     1261 gatgtactcc tcgacgttgg tgtagccgtc gccgtcgcgg tcgcgcgaag cgtcggcggg
     1321 atcgcgcggg tcgaggcggc gcgcggcctc ccattcgtcg ggcatcccgt ccgcgtcgga
     1381 gtctttgggc ggcgcggccc ggcggtactc cggccagccg ccgacctgtt tctgcgagtc
     1441 gatgatggag cctttacgtt ggcgcacctc gtccacgatg cgcgtgtcaa ccgcgtcgcg
     1501 gacgggcagc gtggccccga cctcggccag cactttttcg taagcctccg cggcgggaag
     1561 tgtccgcacc ggctgcgcgg cgaagggcgc ggcgacggtt tgcacctgcc gcttgccgtc
     1621 gatcatcacg gggtcgaaga agagagagtt gtcagcggtg agtttggcgt cgccctcgaa
     1681 gacgttgccg ctgatgtaaa agagtagttc cgaagggccg ccgacgcgga tgggcgtgcg
     1741 ggccttgctg ctggggccgg gccgtatgta gttggcgacg tagttcacct tgaggacgcc
     1801 ctgcgtgagg cccgaagcga tgtcgccgta gtcgtagacg acgttgttgc gcacgtcgaa
     1861 ggtggggtac ggcggcctgc cgttgttgtc gccgagacgc gggttgcgcg cgtcgttgtg
     1921 cgcccacagg ttgtgatgcc acgagacggg gccgttggcg cgggagagcg agccgtagcc
     1981 gtgctcgccc ttctggtgct tgctgcggtt cagcgcctcg gcgatgagac accactgcac
     2041 ggtcacgtcg ctgacgttgc cggtgagcga gagcacttcg tcgatcgacc acgaggccga
     2101 gcagtggtcg aggacgacgt tgcgcgcgcc cgtcccgagc gtgatcgcgt ccgccgcctc
     2161 gccgacgagg tcgcccagac ggctccgcag gtaacgcacg atcacgtcgt gcgtggcgac
     2221 gatgaaggtg tggttgcgca gacagacccc gtcgcccggc gctgtctgcc ccgcgatggt
     2281 gaggtagggg ttcttcacca cgatgggcga ggcgagcgtg atcagaccgg cgacgcggaa
     2341 gacgacgacg cgcgggcctt ccgcctcgca ggccgcgcgc aaggagccgg ggccggagtc
     2401 ttcgaggttg gtgacgagga tgacacggcc cccgcgaccg ccgggcgtcg tggagccgaa
     2461 cccttcggcg ccggggaagg cgggaacggc aggccgctcc gtgggcgctg acgcgacccg
     2521 cgcgggaggc ggcaggaggg tcaacaacag accggcgaga cacaggacga gaccgctcac
     2581 ggctttcatg cgactctctt caatccttcc ccgcttcaag tcgtcgcggc gaactcgatg
     2641 aagttacaga ggagcttgcg cgccacctgc gccgggcctt cctccgacgg aaggttctcg
     2701 acgaggtcga gcgtcgagac gacgatgcgc ccctcgccga cctgtatcac cccgaccgcc
     2761 gtgccgagtt gcatcgggaa actatgccac gccccgacca ccagctcctc gccttccagc
     2821 aggaggccgg ctcgctcgtt gccgttcctg acgaccgcct gatacggcca gttcatcgcg
     2881 ccgcccgccg gcaatccctt aacaagggcg tgcgggcgga cgaaatgcac gccgccgagc
     2941 cacgtgacgc cgaccttgaa cgcgccgccg tatgtgacgg gcgcgttctt gccgaccagc
     3001 cccatccagt cctccgcgta gtcaacgatc atgaccgtcg tcccgtcgcg gcgggcgcgt
     3061 tcgatgagac gctgcgggtc gggcgcgtcg ctcacggggt aagaccacag gagcttgatc
     3121 cggcccgcgc ccctctgctg ccggtactcg acgagaaccg gcacgggccg tcccgcttcg
     3181 agatcgatct cgccgcgttt gtccggcccg cccctcgtcg tcatcgcatc aaccagcacg
     3241 cgcccgttga cggtgaggcg gacgttcccg ctcgtctgga tggagaagac gtgtctaccg
     3301 ctggccgaag gcgtgagctg cccctcccag cggacgccgt agttctcggt ggtcggcacc
     3361 gaagggtcgg gcgtctcgcc ttccgagacg gagaagctga gggtcttatc gactctctgg
     3421 tgaacgcgct gtgcgaaatc ctgaccgagg aagaaggtcg cgagcaggcc cggtttgccc
     3481 gtagggtcgc tgagctgctc ggtggggatc gagcggaacc ctgcgccgct cggcggctgc
     3541 gagacgacga cccagtcgag ccgcccgagg tcgtctttgt acgcgggcgc gtcgagcttc
     3601 ttcaggtttt cgagaaagcc gcggacgtgg ctcccgactt cccacaccgc gcccttgccg
     3661 cgcaagttcg cgcccttcca atcgacgacg aagacttcgt cgtggccgcg cgcctcgacg
     3721 cgccccgcac ggctgacgag ttctgcctcg acgcggaaca tgccgcccgc gccctccgcg
     3781 aaaggcacct ccagcccttc ggcgaggagt tgcccgtagg tgtcgccgcc cgcgagcgtg
     3841 acgggcatct ctttgtcgaa gacgactttc ccggctgtgt cgcgggccga gaccttcagc
     3901 gtgtgcggcc ccttcaggtt cttttcgttg acggcgtaga aatcgaccgc cacgcgcgcg
     3961 ggcgcctgca cgacctgtgt gcggactttg accgcgacgt aaagcggctg gttgtagtgt
     4021 gcgaccaaag cggggtctga cttcgggttg cggaagacat cgacgacgcc cgaatggttc
     4081 tcgatgatct gcgcctccca gccgttgatg gcgtagccgt cgctcgtgtt cgagatgcgt
     4141 atgttctcga tcttgcgccc ctgatggtag agcgagacgg cgcccatctc ggcggtcagc
     4201 gcgtcaacgg tcgggaagac cgtgcgcagt tttttgcgcg tgagaaagtc gtcgaacgcc
     4261 ttgtaccagt cgaggtacat ctggccgtcc caacccttgt tgggcgaagc ggccagcgcc
     4321 tctttgattt tttcgaggcg cggcggcgtg gagatggctc cctcttcgcc ccagaagacg
     4381 atctcgcgcg cgttgtccgt gaggttgtaa tactcgcgcg gattgcggta gaggttctgg
     4441 ctccacacgg cggggccgcc cgcgtggtga tagtcgtacc agccgctcag gtagacgcgt
     4501 tcgtcgaacg ggcgcatgtg catcttgaag gggtcttcca cgtccttggc cgtagcccac
     4561 gcggaggtgc gcgtgatggc gcggctcggg tcgagcgcgt gcgcgtcgcg catgtcctgc
     4621 tcgtgctttt tgacgacggc ctcggtcgtc tgccccatct cgttaatcat gttgtagacg
     4681 acgaggctcg ggtggctgcg gtcgcgccgc accatgcgga ggagcttttc gcgcacgagc
     4741 gcctgcacga agggatcact gcccccggag cggtagttgc ccggctcttc gtaatagagc
     4801 agacccagct cgtccgcctt gtcgagcacg accgcgtggc cgatggcgcg gtgaaagttg
     4861 agcatgttga ggcccatctg tttcgccgcg cgaatctcct gctccgcaag tcccggcgtc
     4921 gggaagatgc cgttgacggg ccagtagccc caactgatgg cggtgcggag cacgacgcgc
     4981 ttgccgttca gacggaagac ggcgtcctgc ccgatgccgg tcggctcgaa ccagcggaag
     5041 ccgaaggtct gccggtcggc gtccgtcgca cggtttccgt tcttcaaaga cgcctcgaag
     5101 acgtaaagat tagggtgctc cgtgtcccag aggtcggctt tgggtgcgga cactttcaag
     5161 gggatgaggt tggcgccggg tttgagcgtc acgcctttga gtaacttgcg gaagatgacg
     5221 gcggacgggt cgcggtgctt gacgacgcgg acttcgaggt cgcggcggac ttccgcggcg
     5281 gcggtgttgc ggacggagac ttcggcgttg acctccgtga ttgccggggt gttctggacg
     5341 tagatgtctg cgacgtaggc ggggtcgcag gagagaagct caacgcggcc cgtgatgccg
     5401 ccgaagccgt ggctgccggg cacctgcacg cgcccccact tgatcgtgtt gccgtcgcgc
     5461 cagtcgaaat tgccgccggg gtccgtgacc cgcaccgcga gccgaacggt cgcgcccggc
     5521 ctcgccacgc ccgagagatc gacctcgaag ggcgtgttgc cgacgaggtc gtagccgacg
     5581 agcttgccgt caacgtaaac ctccgcgcgc agccgcacgg actcgaaacg cagaaggacg
     5641 cggcggggcg acgacgcagc cgggacgacg acggtgcgat accaccagct cacgcccgtg
     5701 aggtcgccct ccggcccggc gacctcctgt aggtactctt cgaccgtacc cggcacggag
     5761 acttctctgc cccggtcggc gttcaagact tcccagccgc cggtcggcgg gttgacgggc
     5821 agcttcgaaa tgtcgggcgc ggggaagaac agctcgtcct gctgccacgc ggcctgcttg
     5881 tcgtgccaca gtctcc
//
LOCUS       DBNGOH010001162         2354 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4761074, whole genome shotgun sequence.
ACCESSION   DBNGOH010001162 DBNGOH010000000
VERSION     DBNGOH010001162.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2354)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2354)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2354
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4761074"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..948)
                     /locus_tag="AC159P_22525"
     CDS             complement(<1..948)
                     /locus_tag="AC159P_22525"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3698276.1"
                     /translation="MNITPPGEVAAVAPAIEFRHVSLSFDGIAALRDVSFTLRRGQMI
                     CITGASGSGKSVLLRLAVGFHKPDGGRIFVGGREIEALGEDELLAIRGGLMGVVFQEE
                     SLFTGLNVYENVAYRPEEHGWAEGETERAVCEALKFVGLEDDIEKLPEELSGGMKRRL
                     EIARAFVGWPSIMLFDEPTAGLDPLNEAQVLDLVLRARDLHGISSLFVTKQLHQIPYL
                     AAHRAAEDPGGSVVIRKAAMTEPPDVKVLFLHKGRVAFFGTPEEFAADTSPEVLYMTR
                     AGSGLHSAGVVVADPWDKKRRPRRTAAKTGVGGALPLKSV"
     gene            1832..>2354
                     /locus_tag="AC159P_22530"
     CDS             1832..>2354
                     /locus_tag="AC159P_22530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3698277.1"
                     /translation="MYSFRKSLAATVGLILVIGLIALFTPSGTRGQSGGLKLPFYEEV
                     SHDKAAFHVHNIAQTSTAIGVYGTGRQGVAGQTSHDVDGVGVIGLGGRGEKSAGVHGR
                     ANNGYGVYGLSVNNIGVVGVRGNSFSDFQTRGILGTKDNAIEGVSKVPGGKGVAGVAH
                     SGANAVGIYGESTG"
BASE COUNT          425 a          765 c          754 g          410 t
ORIGIN      
        1 caccgacttt aggggcaagg ctcccccgac ccccgttttt gctgccgtgc gtctcggcct
       61 gcgcttttta tcccacgggt cggcgacgac gacaccggcg gagtgtaagc cgcttccggc
      121 ccgcgtcatg tacaggactt cgggcgacgt gtccgcggcg aactcctctg gcgtgccgaa
      181 gaacgcgacc cggcccttgt gcaggaacag cactttcacg tcgggtggtt cggtcatcgc
      241 cgcctttctg atgacgacgc tcccgcccgg gtcctccgcg gcgcggtgcg cggcgaggta
      301 agggatttgg tggagctgct tggtgacgaa cagcgaggag atgccgtgca ggtcgcgcgc
      361 gcggaggacg aggtcgagca cctgcgcctc gttgagcggg tcgagtccgg cggtcggctc
      421 gtcgaagagc atgatcgagg gccagccgac gaatgcgcgg gcgatctcca gccgccgttt
      481 catcccgccg gaaagctctt cgggcagctt ctcgatgtcg tcttcgaggc cgacgaactt
      541 gagcgcctcg caaaccgccc gctcggtctc gccctccgcc cacccgtgct cctcgggccg
      601 gtaggcgacg ttctcgtaga cgttgaggcc ggtgaagagc gactcctcct ggaagacgac
      661 ccccatcagc ccgccgcgga tggcgagcaa ctcgtcctcg ccgagcgcct cgatctcgcg
      721 cccgccgacg aagattcgac ctccgtcggg cttgtggaag ccgacggcga ggcgcagcag
      781 cacggactta cccgacccgg aagcgcccgt gatgcagatc atctgccccc gccggagcgt
      841 gaagctgacg tcgcgcagcg cggctatacc gtcgaagctc agcgagacgt ggcggaactc
      901 tatggcgggc gcgacggcgg ccacctcgcc cggcggtgta atgttcatcg acgtactcct
      961 agttgaggtg tttgatcgcc gccccggcga ggctcgtctc cgtcatcaac gcatgagcgc
     1021 cgccggtcaa catgtctttg tctttcacca caaaactcct cttcaagaat tatcccctcc
     1081 acactccagg gcggcgcctg accccgacat gagtgagagc tccgtcctgt ctgtaattag
     1141 agcaacgggc gtgcccgcac ccacccgcgc atttgcgggg cgggaaagcc gagctgagtc
     1201 ggctgtgagg aaacttgcgc acgcacgggc gtaaaagcac gcacatcgcc cgctccggcg
     1261 tgcgtgccgc tagccgacaa actacccgcc gggtttcggt tgatatgctg catgttcgtg
     1321 gcttttatgc acaggctgcc gaataaatag gcaaagcctt cgtcaccttc gattactacc
     1381 actacatgaa cccggtagta cctgactgtc gcattacggt cgaaggctcg ttgacgaagc
     1441 aaactgtctg tcagggcgcc gaaccggggt gcaggatgtg ccgcgacagg cttggtgtcg
     1501 gcaataccga ctcgacgtat gtcgtggtgg ttgctccccc cgccgagaga aaacgcctct
     1561 acgaagacaa tgtcggcagc ctgtagatca gccctgtacg cctgcgcgcc acagcgcgcg
     1621 aaagcggttc acacgcactt tcgatgacgc gatgccggcc aatgcgggcg gcggcgagaa
     1681 tcgttgacgg cgacgccacc gcgggtatag agtccgcgcc acgacaaggg gttctctgcc
     1741 gcggactggc cacacggagc ttcccgacct cctccctctg accggccctt ccatcccgcg
     1801 ccgctcaggc gccctgccaa ggagtaatca catgtacagc ttcaggaagt cactcgccgc
     1861 cacagtcggt ctcatactag tcatcggact gatcgcgctc ttcaccccgt cggggacgcg
     1921 ggggcagtcc ggcggcttga agctcccgtt ctatgaagaa gtgtcgcatg acaaagccgc
     1981 gttccatgtc cataacatcg cgcaaacgag caccgccata ggcgtgtacg ggactggcag
     2041 gcaaggcgtg gccggtcaaa ccagccacga cgtggacggg gtcggcgtga tagggctcgg
     2101 aggccgtggg gagaagtctg ccggcgtcca cgggcgggcc aacaacgggt acggcgttta
     2161 cggcctgagc gtgaacaaca tcggcgtggt cggcgttcgc ggcaatagct tcagtgattt
     2221 ccagacgcgc ggcatcctcg gcactaaaga taacgccatc gagggcgtgt cgaaagttcc
     2281 ggggggcaag ggggtcgcag gcgtggccca cagcggggcg aacgcggtcg gcatctacgg
     2341 cgagagcacc ggcg
//
LOCUS       DBNGOH010001163         3326 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2377782, whole genome shotgun sequence.
ACCESSION   DBNGOH010001163 DBNGOH010000000
VERSION     DBNGOH010001163.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3326)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3326)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3326
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2377782"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="AC159P_22535"
     CDS             <1..342
                     /locus_tag="AC159P_22535"
                     /inference="COORDINATES: protein motif:HMM:NF024751.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0032196 - transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HIR3698278.1"
                     /translation="VDTLCFDAYAEKVLAPRLRRGDVVVLDNLGAHRASRIEEVAESR
                     GARVLWLAPYSPDFGPIEQCRSKIKAYLRGVKARTEDELNKTPAQAIGLVTKADIRGW
                     FKHCGYSSARE"
     gene            complement(401..3007)
                     /locus_tag="AC159P_22540"
     CDS             complement(401..3007)
                     /locus_tag="AC159P_22540"
                     /inference="COORDINATES: protein motif:HMM:NF019334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR3698279.1"
                     /translation="MNPERIEKIDEVFQAALDMASGPRAAFLGEACAGDAELRREVES
                     LLSAHERAGGFMERPVSEEAARLMVGGGAQSLAGRTIKHYKVLKHLGAGGMGEVYLAQ
                     DTRLSRPVALKLLSTHLTTDKERVRRFRQEALTVSALNHPNILTVHEIEEEGDRPFIA
                     TEFVDGVTLRTLMRGRRLSLAEALDIALQVAGALAAAHNAGIIHRDIKPENVMVRPDG
                     LVKVLDFGIAKYAEPARGQDAKESWVRTVTGVIVGTTAYMSPEQARGQAVDARTDIWS
                     LGVLLYETVARRLPFPGKTPTDRVAAILEREPEPLSRLRRGVPAELERSVGRALAKNR
                     DARYARVAEMADDLRKQRAALGDERPYRFALPAPARGLLFYGRRRVVMLAVLLLVMTA
                     ALAAGIYFLRPGAGGAQITSLAVLPLADPGGSSDTEYLSDGITESLIDSLSQLPNLKV
                     MSRNSVFRYKGQEVDARTVGNTLGVSAVLMGRLVRRGDELTVSVELVDAGDNSRLWGG
                     RYSRKMADLLALQSEVTRDVSRKLRARLSGADEQRLAKNYTGNPEAYQLYLKGRYHLL
                     KNTRPEIQTGVSYFRQAIEVDPAYALAHAGLAEAYRLLAIAGEMPPTELMPQAKAAAR
                     KAVEIDDGLAEAHTALGHVIFWHDWDWDAAENRFRRALELDPNSADAHGAYANALSYT
                     GRHAEALAEIKRARELDPLNPRTNVIEGVMLINAGRADEALDRLRKTLQLEPNYWFAR
                     QYSASAYIEKGMYEEAIAEAREAREFPDVPTRPAAFLGYALAKAGRRAEARAELEGLL
                     KLSKERYVPPYNIAMVYNGLGERDEALAWLERGYREREPRMVFLKVEPKWNNLRDDPR
                     FRDLLRRVGFTP"
     gene            complement(3014..>3326)
                     /locus_tag="AC159P_22545"
     CDS             complement(3014..>3326)
                     /locus_tag="AC159P_22545"
                     /inference="COORDINATES: protein motif:HMM:NF019265.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ECF-type sigma factor"
                     /protein_id="HIR3698280.1"
                     /translation="MRHIVVDHARQKRSAKRGGAAHRVTLDEGALFSAGRAAELVALD
                     EALQVLAQLHPRRSRVVELRHFGGLNNREAAEVLGVSEATVERDWRFARAWLYRELER
                     R"
BASE COUNT          501 a         1169 c         1083 g          573 t
ORIGIN      
        1 gtcgatacgc tctgcttcga cgcctacgcc gagaaggtgt tggccccgcg gctgcggcgg
       61 ggcgacgtgg tggtgctcga caacctgggc gcgcaccggg cgagccgcat cgaggaggtg
      121 gccgagagcc gcggggcgcg ggtgctgtgg ctggcgcctt actcgccgga cttcggcccc
      181 atcgagcagt gccggtcaaa gatcaaggct tatctgcgcg gcgtgaaggc gcgcaccgaa
      241 gacgaactca acaagacgcc ggcacaagcc atcgggctcg tgaccaaagc cgacatccgc
      301 ggatggttca agcactgcgg ctactcatca gcacgcgaat gaaagacgcg ataaagtgaa
      361 gtttctcctt ggtctttcat tccttacgac ctcgcaccgt tcaaggcgtg aagccgacgc
      421 ggcgcagcaa atcccggaat cgcgggtcgt cgcgcaggtt gttccacttc ggctcgacct
      481 tcagaaagac catccgcggc tcccgctccc ggtagccacg ctccagccac gcgagcgcct
      541 cgtcgcgctc gcccagcccg ttgtagacca tcgcgatgtt gtaaggcggg acgtaacgct
      601 ccttcgataa cttcaacagc ccttcgagct cggcccgcgc ctctgcccgc cggcccgcct
      661 tcgccaacgc gtagccgagg aatgccgcgg ggcgggtggg aacgtcggga aactctctcg
      721 cctcgcgagc ttcggcgatg gcttcctcat acatcccctt ctcgatgtag gcgctggcgg
      781 aatactgccg cgcgaaccaa tagttcggtt caagttgcag agtcttccgc aacctgtcca
      841 gcgcctcgtc ggcccgcccg gcgttgatga gcatcacccc ttcgatgacg ttggtcctcg
      901 ggttgagcgg gtcgagttcc cgggcgcgct tgatctcggc gagtgcttcg gcgtgccgcc
      961 ccgtgtagga gagtgcgttc gcgtaagccc catgagcgtc cgcgctgttc gggtcgagtt
     1021 ccagggcacg cctgaaccga ttctcggctg cgtcccagtc ccagtcgtgc cagaagatga
     1081 catggccgag ggcggtgtgg gcctcggcca gcccgtcgtc gatctcgacc gctttgcgcg
     1141 ccgcggcctt cgcctgcggc atcagttccg tcggaggcat ttcaccggcg atcgcaagca
     1201 ggcggtacgc ctcggcgagc ccggcgtggg cgagcgcgta agccgggtct acctcgatcg
     1261 cctgccggaa gtaggagacg ccggtctgga tctccggccg tgtgtttttg agaaggtggt
     1321 aacgaccctt gaggtaaagc tggtaggcct cggggttccc cgtgtaattc ttcgccaggc
     1381 gctgctcgtc ggcgcccgac agccgcgccc gcaacttccg cgaaacgtcc cgcgtgacct
     1441 cgctctgcag cgccaggagg tctgccattt ttcggctgta ccgcccgccc cagaggcggc
     1501 tgttgtcgcc tgcgtccacc agctccacgc tgacggtaag ctcgtcgccg cgccgtacga
     1561 gccggcccat caacacggcg ctcacaccga gggtgttccc caccgtccgg gcgtccactt
     1621 cctgcccctt atagcggaag acggagttgc gcgacatgac cttcaggtta ggcagttgcg
     1681 agaggctgtc gatgaggctt tcggtgatgc cgtccgagag gtactccgtg tcggagctgc
     1741 cgccggggtc ggcaagcggc aggacggcga gcgaagttat ctgcgcgccg ccggcaccgg
     1801 ggcggagaaa atagatgccg gcggcgagcg cggctgtcat gacaagcaga agcaccgcca
     1861 gcatcaccac ccttcgtcgg ccgtagaaga gtagcccgcg cgccggcgcg ggcagggcga
     1921 agcggtaggg gcgctcgtcg cccagcgccg cccgctgctt gcgtaaatca tccgccatct
     1981 cggcgacgcg cgcataccgc gcgtcccggt tcttcgcgag cgcgcgcccg acgctccgtt
     2041 cgagttcggc cggcacgccg cgccgcagcc tgctcagcgg ctctggctcg cgctccagga
     2101 tggcggccac gcggtcggtc ggcgtcttgc cggggaaggg gaggcggcgg gcgaccgtct
     2161 cgtagaggag cacacccagc gaccagatgt cggtgcgcgc gtccacggcc tgaccccgcg
     2221 cctgttcggg cgacatgtag gccgtcgtcc cgacgatgac gcccgtcacc gtcctgaccc
     2281 aagactcttt cgcgtcctgc ccgcgggccg gctcggcgta cttggcgatg ccgaagtcga
     2341 ggaccttcac cagcccgtcg ggcctgacca tgacgttctc gggcttgatg tcgcggtgga
     2401 tgatcccggc gttgtgcgcc gccgccaggg cgccggcgac ctggagcgcg atgtcgagcg
     2461 cctcggcaag tgacagccgc ctgccgcgca tgagcgtgcg cagcgtcacc ccgtccacga
     2521 actccgtggc gatgaaaggt ctgtcaccct cctcctcgat ctcgtggacg gtgaggatgt
     2581 tcgggtggtt gagggccgag accgtgagcg cctcctgccg gaagcggcgc acccgctctt
     2641 tgtcagtcgt caggtgtgtg gaaagcagtt tgagtgcgac cggacgactc agtcgtgtgt
     2701 cttgcgcgag gtagacctcg cccatcccgc ccgcgcccag atgtttgagc accttgtagt
     2761 gtttgatcgt ccggccggcg agcgactgcg cgccgcctcc gaccatcagt cgggcggctt
     2821 cctcggacac cggccgctcc ataaaccccc ccgcccgctc gtgcgccgag agcagggact
     2881 cgacctcgcg ccgcagctcc gcatcgcccg cgcaggcctc gccgaggaag gcggctctcg
     2941 gccccgacgc catgtcgagc gccgcctgga agacctcatc tattttctct atgcgctccg
     3001 ggttcatgcc cattcaccgc cgctcgagct cgcggtagag ccacgccctg gcgaacctcc
     3061 agtcgcgctc gaccgtcgcc tccgacaccc cgagcacctc tgccgcctcc cggttgttga
     3121 gccctccgaa gtggcgcaac tcgacgaccc tcgaccggcg cgggtgaagc tgcgccaaca
     3181 cttgcagcgc ctcgtcgagc gcgaccagct ccgctgcgcg ccccgcggaa aacaatgccc
     3241 cctcgtcgag cgtcaccctg tgcgccgcgc cgccccgctt ggccgaccgc ttctgccgcg
     3301 cgtggtcgac gacgatgtgg cgcatc
//
LOCUS       DBNGOH010001164         3656 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3113217, whole genome shotgun sequence.
ACCESSION   DBNGOH010001164 DBNGOH010000000
VERSION     DBNGOH010001164.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3656)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3656)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3656
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3113217"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..843
                     /gene="acnA"
                     /locus_tag="AC159P_22550"
     CDS             <1..843
                     /gene="acnA"
                     /locus_tag="AC159P_22550"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007360500.1"
                     /note="catalyzes the conversion of citrate to isocitrate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HIR3698281.1"
                     /translation="LWNDIPSVTGEVYQWDPQSTYIQEPPYFEKFGMQPGVFCDIKAA
                     RPLAIFGDSVTTDHISPAGAIKPKSPAGRYLMLRGVSVEDFNSYGSRRGNHQIMVRGT
                     FANVRIKNLMVPGTEGGVTALEPVGEQMSIYDASVNYQAANIPLVVIAGQEYGTGSSR
                     DWAAKGTKLLGVRAVIAESFERIHRSNLVGMGVLPCQFKEGTNAQTLNLDGSEIFDLV
                     GLESGDIRPRQDVTLRITRENGETEEVPVTLRIDTPIEIEYYRHGGILPFVLRQLIGQ
                     STEQ"
     gene            959..2134
                     /locus_tag="AC159P_22555"
     CDS             959..2134
                     /locus_tag="AC159P_22555"
                     /inference="COORDINATES: protein motif:HMM:NF019500.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005216 - monoatomic ion channel activity
                     [Evidence IEA];
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HIR3698282.1"
                     /translation="MRTILFTAAGAALLALIAFEVYATVLHSTAHFGPIGERLNRVVW
                     RASCYVARRLSRTRRHRFLNIVGPLLMPTLIVGLVVTLVFSFALIYYPRMPAEFIFTS
                     RPAGGDFFDAIYFSGVTLTSVGYGDIVPGTNPMRLLAMIEGAAGLALITLAIAYLLMI
                     YTALERKRAVALSFYHQAGEGADAAGYIAHHFVEGKFYGLRESLREATRDIQGLLESH
                     VEHPVINYFHPFEVYKGFPRIVFLLLEICAVSRTSLDPGQYSELRNYPEVRTLEANTR
                     HVLDELVASLGLERRERERDDKRPADERRWLKRYRLNLSRLAEAGIETREDAARGFAE
                     YRALRQEWEDKLQRLSNYLGYDWDEITGDLDPDYAVNEEKEEPEAGEGKRQEAKGKS"
     gene            2275..>3656
                     /locus_tag="AC159P_22560"
     CDS             2275..>3656
                     /locus_tag="AC159P_22560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIR3698283.1"
                     /translation="MSSTQPASETPRKYQLLIDGQWVDAESGKTFETPNPATGQKLAE
                     VAEGDKADIDKAVEAARRAYDGKWSKMSARDRGRLLYKLSQLIEAKSQELAAMETADN
                     GKPIKETSYVDLPQVVENFEYFAGWATKIEGETIPVPGQMFNYTLREPVGVCGQIIPW
                     NFPLLMAAWKLAPALAAGNTVVLKPAEQTPVTAMELGKLIQEAGFPDGVVNIVPGYGE
                     TAGAALAAHPGVDNIAITGTTEVGKIIARAAADKLKKVSLELGGKAPNIVFADADLEQ
                     AVNGAMMGIFFNQGQVCCAGSRLFLDERIKDEFLARFKEKAEKIKVGDPMSKETHMGP
                     QVSEEQLTKIKGYVDIARGEGATVYAGGTPPALEGDFQQGYFFQPTIFSDVQNQMRVA
                     QEEIFGPVTSVITFTDEQDLIKQANETIYGLSAGIWTRDITRAHRFAKEVKAGVVWIN
                     TYNMFNAASP"
BASE COUNT          690 a         1257 c         1138 g          571 t
ORIGIN      
        1 ctctggaacg acatcccctc ggtgacgggc gaggtctacc agtgggaccc gcaatcgacc
       61 tacatccagg agccgcccta cttcgagaag ttcggcatgc agcccggcgt cttctgcgac
      121 atcaaggccg cgcgcccgct cgccatcttc ggtgactcgg tgacgaccga ccacatctcg
      181 cccgccgggg ccatcaagcc gaagtcgccc gccggtcgtt acctgatgct gcgcggcgtg
      241 tcggtcgagg acttcaactc ctacggctcg cggcgcggca accaccagat catggtgcgc
      301 ggcacgttcg ccaacgtccg catcaagaac ctgatggtgc cgggcacgga gggcggcgtc
      361 accgcgctcg aacccgtcgg cgagcagatg agcatctacg acgcctcggt gaactatcag
      421 gcggcgaata tcccgctcgt cgtcatcgcg gggcaggagt acggcaccgg ctcttcgcgc
      481 gactgggcgg cgaagggcac gaagctgctg ggcgtgcgcg ccgtcatcgc cgagagcttc
      541 gagcgcatcc accgctcgaa cctcgtcggt atgggcgtgc tgccgtgcca gttcaaagag
      601 ggcaccaacg cccagacgct caacctcgac ggctccgaaa tcttcgatct cgtcggcctc
      661 gaaagcggcg acatccggcc ccggcaggac gtcacgctgc gcatcacgcg cgagaacggc
      721 gagaccgaag aggtgcccgt cacgctccgc atcgacaccc ccatcgagat cgaatactac
      781 cgccacggcg gcatcctccc cttcgtcctg cggcagctca tcggacagtc aacagaacag
      841 taggcagcag gcagtaggca gtcaacacct tctctatcga acggtcgtgg cgcaagcgtc
      901 aacgtcttgc tcgctcaacc aactgcctac tgcctgctgc ctactgccta ctgcttttat
      961 gcgaaccatt ctcttcacgg cggcgggcgc ggcgctgttg gcgctgatcg cgttcgaggt
     1021 ctacgcgacg gtgctgcact cgacggcgca cttcggcccc atcggcgagc ggctgaaccg
     1081 cgtggtgtgg agggcgtcgt gctacgtggc gcggcgcttg tcgcggacgc ggcggcacag
     1141 gttcctcaac atcgtcggcc cgctgctgat gccgacgctg atcgtggggc tggtcgtcac
     1201 gctcgtcttc tccttcgcgc tcatctacta cccgcggatg cccgccgagt tcatcttcac
     1261 gagccggccc gcgggcggcg acttcttcga cgccatctac ttcagcggcg tgacgctgac
     1321 ctcggtcggc tacggcgaca tcgtgccggg cacgaacccg atgcggctgc tggcgatgat
     1381 tgagggcgcg gcgggcctcg ccctcatcac gctcgccatc gcctacctgc tgatgatcta
     1441 cacggcactg gagcgcaagc gcgccgtcgc gctctccttc taccaccagg cgggcgaggg
     1501 cgcggacgcc gccggctaca tcgcgcacca cttcgtcgag ggcaagttct acggcctgcg
     1561 cgagtcgctg cgcgaagcca cgcgcgacat tcaggggctg ctcgaatcgc acgtcgagca
     1621 cccggtcatc aactacttcc acccgttcga ggtttacaaa ggcttcccgc gcatcgtctt
     1681 cctcctgctc gagatctgcg ccgtcagtcg cacctcgctc gaccccggcc aatactcgga
     1741 gctgcgcaac tacccggagg tgcgcacgct cgaagccaac acgcgccacg tgctggacga
     1801 gctggtcgcc tcgctcggcc ttgagcggcg cgagcgcgag cgcgacgaca agcggcccgc
     1861 cgacgagcgg cgctggctga agcgctaccg cctcaacctc tcgcgcctcg ccgaggcggg
     1921 catcgagacg cgcgaggacg cggcgcgcgg cttcgcagag taccgcgccc tgcgccagga
     1981 gtgggaggac aagctgcaac gactctccaa ctacctcggc tacgactggg acgagatcac
     2041 cggcgacctc gaccccgact acgccgtcaa cgaggagaag gaagagccgg aagcgggcga
     2101 aggcaagagg caagaggcaa aaggcaaaag ttaaaagggc ggacgcgcct gcggcgtgac
     2161 ttttgcctct tgcctcttgc cttttgcctt cggcgcagcc ggtggtaaag ttgcacgcaa
     2221 cgcaggctta tctcgcaacc cgaatccgaa gcaagaaccg aaggagtgca gtttatgtca
     2281 tcaacacaac cggcgtcgga gacgcctcgc aaataccaac ttttgatcga cgggcagtgg
     2341 gtggacgccg agtccggcaa gacgttcgag acgccgaacc ccgcgaccgg gcagaagctc
     2401 gcggaggtag ccgagggcga caaggccgac atcgacaagg cggtcgaggc cgcgcgccgc
     2461 gcctacgacg gcaagtggtc gaagatgtcc gcgcgcgacc gcgggcggct gctctacaag
     2521 ctgtcgcagc tcatcgaagc gaagtcgcag gagctggccg cgatggagac cgccgacaac
     2581 ggcaagccca tcaaggagac ctcctacgtt gacttgcctc aggtggtcga gaacttcgag
     2641 tacttcgccg gctgggcgac caagatcgag ggcgagacca tccccgtccc cggccagatg
     2701 ttcaactaca ctttgcgcga acccgtcggc gtctgcggcc agatcatccc gtggaacttt
     2761 cccctcttga tggccgcgtg gaagctcgcg cccgcgctcg ccgcgggcaa cacggtcgtc
     2821 ctcaaacccg ccgagcagac gcccgtcacg gcgatggaac tcggcaagct gattcaggag
     2881 gcgggcttcc ccgacggcgt cgtcaacatc gtccccggct acggcgagac ggcgggcgcg
     2941 gcgctcgccg cgcaccccgg cgtcgataat atcgccatca caggcacgac cgaggtcggc
     3001 aagatcatcg cgcgagcggc ggctgacaaa ctcaagaagg tctcgctcga actcggcggc
     3061 aaggcgccca acatcgtctt cgccgatgca gacctggagc aggccgtcaa cggcgcgatg
     3121 atgggcatct tcttcaatca ggggcaggtc tgctgcgccg gctcgcgcct cttcctcgac
     3181 gagcgcatca aggacgagtt cctcgcgcgc ttcaaggaga aggccgagaa gatcaaggtc
     3241 ggcgatccga tgagcaaaga gacgcacatg gggccgcagg tctccgagga gcagttgacc
     3301 aagatcaagg gctacgtgga tatcgcgcgc ggcgaaggcg cgaccgtcta cgccgggggc
     3361 acgcctcccg cgctcgaagg cgacttccag cagggctact tcttccagcc gaccatcttc
     3421 agcgacgtgc agaaccagat gcgcgtcgcg caggaagaaa tcttcggccc cgtcacctcc
     3481 gtcatcacct tcacggacga gcaggacctc atcaagcagg ccaacgagac catctacggc
     3541 ctctccgcag gcatctggac gcgcgacatc acgcgcgccc accgcttcgc caaagaggtc
     3601 aaggcgggcg tggtctggat caacacctac aacatgttca acgcggcctc gccctt
//
LOCUS       DBNGOH010001165         1713 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4761243, whole genome shotgun sequence.
ACCESSION   DBNGOH010001165 DBNGOH010000000
VERSION     DBNGOH010001165.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1713)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1713)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1713
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4761243"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(215..1171)
                     /locus_tag="AC159P_22565"
     CDS             complement(215..1171)
                     /locus_tag="AC159P_22565"
                     /inference="COORDINATES: protein motif:HMM:NF024816.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3698284.1"
                     /translation="MNSRRKSPKLLIPAALALAAAVCLVAAGVKAVRDEAHATGGGES
                     AGLRVQSLVMRAVRLREAGRYAEAGPPLREALALAEKSFGHDSFATAAVLNQLGVLGK
                     YDGHFDQAEAAYRRALRITEQTVGLEHPLAATLFHNLGGLEHARGRFAAGEPHARRSV
                     EIRERLVGPDHTDTAADVAALAALLDGQGKYEEAEPLYRRALSIFERAYGPEHYEVAV
                     NLNNLAALCHATGRAEEAEALYRRSLAIKEKLLGAQHPDIGVTLNNLAVFYKSRQRYA
                     EAADSYRRALAIFEGALPPSHPKLATCRENYERLLSDMDQRG"
     gene            complement(1203..1634)
                     /locus_tag="AC159P_22570"
     CDS             complement(1203..1634)
                     /locus_tag="AC159P_22570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3698285.1"
                     /translation="MNAGTGLVAIVSLLLALAGAGGVATEQAEVNSQAARPVTTSKIA
                     LNHNETLVSDATPVEQADGSLYAPKKAGCPVWMCGTNHNETLMSDAPPVEQADTWGRW
                     LTSVHSFVIERFSTYAPRPVLCEDWGCGMNHNETMVSDAAR"
BASE COUNT          248 a          539 c          582 g          344 t
ORIGIN      
        1 gtgccgagtg ggcggtagga gtcgcttcgc tgcccgacgg gtaggcgcgc acacacgtga
       61 gggcgacgcc aaggctgatc gcgatcacga atcctgtgtt ccgtgttttg actttcatgt
      121 tcgatcccct ttcacttcgt tgtcgtagtt gatttcgctg acgtagctcg cggatacaaa
      181 gggcagatac tcttcacttt ttactaaccg gcattcaccc gcgttggtcc atgtcgctga
      241 gcaggcgctc gtaattctcg cggcaggtcg cgagcttcgg gtgcgaaggc gggagcgccc
      301 cctcgaagat ggcgagggcg cggcggtaag aatcggcggc ctccgcgtac ctttgccgcg
      361 acttgtagaa gaccgccaga ttgttgagcg tgacgcctat gtccggatgc tgcgcgccga
      421 gcagtttctc cttgatggcg agcgagcgcc gatacaatgc ttcggcttcc tccgcgcgtc
      481 cggtggcgtg gcacagcgcc gcgaggttgt tgaggttgac ggccacctcg tagtgctccg
      541 gcccgtaggc gcgctcgaag atcgagaggg cgcggcggta gagcggctcc gcctcttcgt
      601 acttgccttg gccgtcgagc agtgcggcga gcgccgccac gtccgccgcg gtgtccgtgt
      661 ggtcggggcc gacgagccgt tcgcggattt cgacggagcg gcgggcgtgt ggctcgcctg
      721 cggcgaagcg cccgcgggcg tgctccaacc cgccgaggtt gtggaagagt gtggcggcga
      781 gcgggtgttc gaggccgacg gtctgttccg tgatgcggag ggcgcggcgg taggcggctt
      841 ccgcttggtc gaagtggccg tcgtacttgc cgagcacgcc aagctggttg agaacggcgg
      901 cagtagcgaa cgagtcgtgg ccgaaggact tttcggccag cgcgagcgct tcgcgcagcg
      961 gcggcccggc ctccgcgtaa cggcctgctt cgcggagccg gacggcgcgc atgacgagcg
     1021 attgcacgcg gaggccagcc gattcgccgc cgcccgtggc gtgtgcctcg tcgcgcaccg
     1081 ctttgacgcc cgccgccacg aggcagacgg cggcggcgag cgcgagcgcg gcggggataa
     1141 gcaacttcgg cgattttctt cgtgagttca tggctgcgcc tcttccgtcc atgtctgtca
     1201 cttcatcgag ctgcgtcact caccatcgtc tcgttgtggt tcatgccgca tccccagtcc
     1261 tcgcagagaa ccggcctggg tgcgtaggtc gagaacctct cgatgacgaa cgagtgcaca
     1321 ctcgtcaacc accggcccca tgtgtccgcc tgctctaccg ggggtgcgtc gctcatcaac
     1381 gtctcgttgt ggttcgtgcc gcacatccat acggggcagc cggccttctt cggcgcataa
     1441 agtgaaccgt ccgcctgctc gaccggcgtc gcgtcgctca ccagcgtctc gttatggttg
     1501 agagctatct ttgaagtggt gacgggccgc gccgcctggg agttcacctc cgcctgctct
     1561 gtggccacgc cgccggcccc ggccagcgcc aacaaaagcg aaactatcgc cacgagtccc
     1621 gttcctgcgt tcatgatctt ttctcctttc agtgttcgcg ttgccgtctc tacccgttaa
     1681 ggcgggaacg gggggcgaga gctgtaacgg cgg
//
LOCUS       DBNGOH010001166         1834 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4943660, whole genome shotgun sequence.
ACCESSION   DBNGOH010001166 DBNGOH010000000
VERSION     DBNGOH010001166.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1834)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1834)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1834
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4943660"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..529)
                     /locus_tag="AC159P_22575"
     CDS             complement(<1..529)
                     /locus_tag="AC159P_22575"
                     /inference="COORDINATES: protein motif:HMM:NF013584.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HIR3698286.1"
                     /translation="MSMKRDEEARDGLPVGRREFIRAGVLGGLAAVAAPALSASLDAA
                     SFAPQSSELEEATIAQLQEWMGSGRRSSREIAEGYLERVEKIDRRGPSVNSVIETNPD
                     ALSIAAALDRERKSGRVRGPLHGVPVLIKDNIDTADRMMTTAGSLALVGAKPLRDAVV
                     VQQLIAGGAVIIGKTN"
     gene            complement(558..1817)
                     /locus_tag="AC159P_22580"
     CDS             complement(558..1817)
                     /locus_tag="AC159P_22580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase-like protein"
                     /protein_id="HIR3698287.1"
                     /translation="MNKTATQSATGVLSTSEIRSQFPALERAHGGRAVAYFDGPGGTQ
                     VPRAVVEAVSEYLYHHNANTHWAYPTSAETDEMIEAARSTLADFLNASPHEVIFGANM
                     TTLAFHLSRTLGRTFSPGDRVVVTELDHHANVAPWQALARERGVEVSVVRMLTEAGEL
                     DWEDFERKVTARTKLVAVGAASNALGTINDLGRAAGLARAVGAYLFVDAVHSAPHRLA
                     DVRAIGCDFLGCSAYKFYGPHIGAMYCRRELLEALPFPKLLPAPETAPERAETGTQNH
                     EGIVGAAAAVNFLASLAPDDEAPRRERLRAVFDALHERGTALTKKLWDGLARIDGVRL
                     YGPPPDAPRTPTVAFTVRGVTSTEVARRLAARGVFVSHGDFYANTVIERLDLGEEGLV
                     RAGCACYTNDEEIERLVEGIEEASGGR"
BASE COUNT          253 a          603 c          675 g          303 t
ORIGIN      
        1 ggttagtctt gccgatgatg acagcgccgc ccgcgatcaa ctgctgcacg acgacggcgt
       61 cgcgcaaggg cttcgcgccg acgagcgcga gcgagccggc ggtggtcatc atccggtcgg
      121 cggtgtcgat gttgtctttg atgagaacgg ggacgccgtg gagcggcccg cgcacgcgcc
      181 cgcttttgcg ctcgcggtcg agcgcggcgg cgattgagag cgcgtccggg ttcgtctcga
      241 tgacggagtt gacggagggg ccgcgccggt cgatcttctc gacgcgctca agatatcctt
      301 cggcgatctc gcgcgaagag cggcggcccg accccatcca ctcttgaagt tgcgcgatgg
      361 tcgcctcttc cagctcggac gactgcggcg cgaaagacgc ggcgtcgaga gacgcggaga
      421 gcgccggggc ggcgacggcg gcgagaccgc cgagcacgcc cgcgcggatg aattcgcgcc
      481 gacccacggg caagccgtcg cgcgcttctt cgtcacgttt catcgacatc tctctctcct
      541 cgcgttccgc cttcacctca acgcccgccg ctcgcttcct cgatgccttc gacgagtcgt
      601 tcgatctctt cgtcgttcgt gtagcaggcg cagccggcgc gcaccagccc ttcttcgccg
      661 aggtcgaggc gttcgatgac ggtgttcgcg tagaagtcgc cgtgcgagac gaagacgccg
      721 cgtgcggcga ggcggcgcgc gacctcggtc gaggtcacgc cgcgcacggt gaaggcgacg
      781 gtcggcgtgc gcggcgcgtc gggcggcggg ccgtagagcc ggacgccgtc gatgcgcgcg
      841 aggccgtccc agagtttttt cgtcagcgcc gtgccgcgtt cgtggagcgc gtcgaagacc
      901 gcgcgcagtc gttcgcggcg cggcgcctcg tcgtccggcg cgagcgaggc gaggaagttg
      961 acggctgccg ccgcgcccac gatgccttcg tggttctgcg tgccggtctc ggcgcgttcg
     1021 ggcgcggtct cgggcgcagg gagtagcttg gggaagggga gtgcttcgag cagctcgcgg
     1081 cggcagtaca tcgcgccgat gtgggggccg tagaacttgt aggccgagca gccgaggaag
     1141 tcgcagccta tggcgcgcac gtccgcgaga cggtgcggcg cggagtggac ggcatcgacg
     1201 aagaggtagg cgccgaccgc gcgcgccagc cctgccgcgc gcccgaggtc gttgatcgtc
     1261 ccgagcgcgt tcgaggccgc gccgacggcg acgagcttcg tccgcgccgt gaccttgcgc
     1321 tcgaagtctt cccagtcgag ttcgcccgcc tccgtcagca tgcggacgac cgagacctcg
     1381 acgccgcgct cgcgtgcgag cgcctgccac ggcgcgacgt tggcgtggtg gtcgagttcg
     1441 gtgacgacga cgcggtcacc cggagagaac gtgcgcccga gcgtgcgcga aaggtggaag
     1501 gcgagcgtcg tcatgttcgc gccgaagatg acctcgtgcg gcgacgcgtt caggaagtcg
     1561 gcgagcgtcg agcgcgccgc ttcgatcatc tcgtccgtct cggcgctcgt cgggtaggcc
     1621 cagtgcgtgt tggcgttgtg gtggtagagg tactcgctca ccgcctcgac gaccgcgcgc
     1681 ggcacctgcg tcccgccggg gccgtcgaag tacgcgaccg cccgcccgcc gtgcgcgcgt
     1741 tcgagcgcag ggaactgcga cctgatctcc gaagtggaga gaacgcctgt cgccgactgc
     1801 gttgctgttt tgttcatgtg tcaatccaga agta
//
LOCUS       DBNGOH010001167         5342 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_727513, whole genome shotgun sequence.
ACCESSION   DBNGOH010001167 DBNGOH010000000
VERSION     DBNGOH010001167.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5342)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5342)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5342
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_727513"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="AC159P_22585"
     CDS             <1..234
                     /locus_tag="AC159P_22585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3698288.1"
                     /translation="ISTLDQTTLKVPILTCGGGGTGCHIKPTPPKKILNIEIDKKNAD
                     PAFNCAKCHVNFGKVPKEQIPKSHTDAVSAPKK"
     gene            250..1224
                     /locus_tag="AC159P_22590"
     CDS             250..1224
                     /locus_tag="AC159P_22590"
                     /inference="COORDINATES: protein motif:HMM:NF025875.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HIR3698289.1"
                     /translation="MNGEPHNEPRDPRSDPPRAAPSHAHHASPSALAQRRRVLSLASA
                     LAVCAGLFLTSAVFSRGRATSAEASAEPAPVSSFEPQDGSFTKFPHQTGAHAALACDS
                     CHRRENNAAQPTMPGGNGHAACINCHQQQFFTPSHPMCTICHTDLGSSNPPVKAFPRL
                     ASFNVKFDHAQHLRGAARPSAGCASCHAPMRRGVALSIPAGLSAHNNCYQCHTPGANS
                     GGRDIGSCGACHSLGGYRRTPTTARAYAVSFSHATHGPRQGLRCDECHSVRAGAAQSR
                     QVTSPTPSQHFASARAQSCMTCHNNRRTFGGDDFDDCKRCHKGATFRF"
     gene            1365..1976
                     /locus_tag="AC159P_22595"
     CDS             1365..1976
                     /locus_tag="AC159P_22595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HIR3698290.1"
                     /translation="MKLFIVLAAVACYICIAVGCNPSSLKSGAGALAAFDGRVPKDDY
                     VLSADAKKPADAPPVPFSHKNHSEKNYSIDGTKVIGCVECHHTDQPAAEAAKKPPLKT
                     GWPADRTTTLTTDLLAKDPKAPDVISCRSCHAQEGAKPKVASAGPDIPKVTYEGDTDA
                     TELDNEEAYHRNCNTCHDLAVEARKQLKIPTSQECAKCHSGKK"
     gene            2084..2605
                     /locus_tag="AC159P_22600"
     CDS             2084..2605
                     /locus_tag="AC159P_22600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3698291.1"
                     /translation="MRYLSFAPVLALCLLYAQCGGSPPADNARRNSNAAPAANTAPAA
                     NTSAPPVASSHGGGGTANSGGAAPGGAAAPDKPPVATPELDEKIEKAEAKAKTAGAAA
                     ADKQAAAAAHMARGNFYYNAQDKRLYRYALGDFRRALRYEPDNAEAKEKIEMIESIYR
                     SLGRPVPTNGLEP"
     gene            complement(2702..3106)
                     /locus_tag="AC159P_22605"
     CDS             complement(2702..3106)
                     /locus_tag="AC159P_22605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3698292.1"
                     /translation="MLKRLVSLSIAALLLHAADVFPLPALARTAQSEQQAAKVKADVT
                     RRGTGKKARVVVRLQDGSKLKGYVSQAGNDSFTLTDSRSGQTRTLSYGDVARVNKPGG
                     LPLGAKIGIGMGALMGVLALVYAAGCHDTSAC"
     gene            complement(3299..3475)
                     /locus_tag="AC159P_22610"
     CDS             complement(3299..3475)
                     /locus_tag="AC159P_22610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1059 domain-containing protein"
                     /protein_id="HIR3698293.1"
                     /translation="MARTMNCRDVGPDCDFVARGETDDEVMGQVAEHARTAHGLDEVP
                     PELAEKAKAAIKDE"
     gene            complement(3565..5133)
                     /locus_tag="AC159P_22615"
     CDS             complement(3565..5133)
                     /locus_tag="AC159P_22615"
                     /inference="COORDINATES: protein motif:HMM:NF019604.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HIR3698294.1"
                     /translation="MKKAQLFVTLALLTLLVAVNQYSQDPTKKFFGGLPWWGWAAIAL
                     FLLVIAIGFALRDSRRARLLLEEPPQEKKKERQAELITEEQRRMYDPDGPDYPHPVII
                     KDRCIGCHACVDACPHDVLAIVDNISSPIAPDQCMEDTSCQVECPVNPKACIVVNTTK
                     KIKPRPVPNRDAKFMTDVPGCFIIGDVSGTPLIKNAANEGADVVKYISHELRNNGAQP
                     EPKAELDVAIIGIGPAGLSAAITAKQHQLSYVGIEQDKVLATIDAYPANKYVFFKPET
                     MESRGAIDVNSAGRQREEILSDWIGTMRKSEVVINETESCKSVKKAEDGDYFVIQTEK
                     TIKNEGGETKEKVTYKARRVVLALGNRGTPMKLKVPGEEMKVTRDGETEDKVKYKLTD
                     PEEYRRRKMIVVGAGNSAIEAAVDLVARRKGTAITFRPDEEINEVTLVIRSDMKNDLK
                     FGNKQQVYDCIDEGKIKVYFGTAIKEIHDGEVVLMNARTEEVKATVPNDFIFALIGGD
                     KPTRFLESIGVKIG"
BASE COUNT          890 a         1908 c         1661 g          883 t
ORIGIN      
        1 atcagcacgc tcgatcagac gacgctcaaa gtccccatcc tcacctgcgg cggcggcggg
       61 acggggtgcc acatcaagcc gacgcccccg aagaagattc tcaacatcga gatcgataag
      121 aagaacgccg accccgcgtt caactgcgcg aagtgccacg tcaacttcgg caaggtgccg
      181 aaggagcaga taccgaagtc gcacaccgac gcggtctccg cgccgaagaa ataaccccgg
      241 acatcgatgg tgaacggcga accacacaac gaacccagag acccgcgcag cgacccgccg
      301 cgcgccgccc cgtcgcacgc gcaccacgct tcgccgtccg cgctcgcgca gcggcggcgc
      361 gtcttgtcgc tcgcgtccgc gctcgccgtc tgcgccggac tctttctcac ctccgccgtc
      421 ttctcgcgcg ggcgcgcgac ttccgccgag gccagcgccg aacccgcgcc cgtctcttcg
      481 ttcgagccgc aggacggcag cttcacgaag ttcccgcacc agacgggcgc gcacgccgcg
      541 ctcgcctgcg actcctgcca ccggcgcgag aacaacgcgg cgcagcccac catgccgggc
      601 ggcaacggcc acgccgcgtg catcaactgc caccagcagc agttcttcac gccgagccac
      661 ccgatgtgca cgatctgcca cacagacctc ggctcgtcga acccgccggt caaagccttc
      721 ccgcgcctcg ccagcttcaa cgtcaagttc gaccacgcgc aacacctgcg cggcgccgcg
      781 cgcccctccg ccgggtgcgc ctcgtgccac gcgccgatgc gccgcggcgt cgcgctctcg
      841 atcccggcgg gcctcagcgc ccacaacaac tgctaccagt gccacacgcc cggcgccaac
      901 tcgggcgggc gcgacatcgg ctcgtgcggc gcctgccaca gcctcggcgg ctaccggcgc
      961 acgccgacga ccgcgcgcgc ctacgccgtc agcttcagcc acgccacgca cggcccccgt
     1021 cagggcttgc gctgcgacga gtgccacagc gtccgcgcgg gcgccgcgca gtcgcgccag
     1081 gtcacttcgc ccacgccctc gcagcacttc gccagcgcgc gcgcgcagag ctgcatgacc
     1141 tgccacaaca accgccgcac cttcggcggc gacgacttcg acgactgcaa gcgctgtcac
     1201 aagggcgcca ccttccgctt ctgatacgcg cccggaaaaa ctcgattgac aagcttggag
     1261 cgccgcgtct atgattcacg gcaccggctt tgacaacatc cgcgactccc cctcttcgag
     1321 ctttttttga aatcatcgct tcgaaagacc gaggactctc gaacatgaaa cttttcatcg
     1381 tgctggccgc tgtcgcctgc tacatctgca tcgcagtggg ttgtaacccc tcgtcgctca
     1441 agagcggcgc gggcgcgctc gccgccttcg acgggcgcgt gccgaaggac gattacgtcc
     1501 tctcggctga cgcgaagaag cccgccgacg cgccgcccgt ccccttcagc cacaagaacc
     1561 actccgagaa gaactacagc atcgacggca cgaaggtcat cggctgcgtc gagtgccacc
     1621 acacggatca gcccgccgcc gaggccgcca agaagccgcc gctcaagacc ggctggcccg
     1681 ccgaccgcac gaccaccctg accacagacc tgctggccaa agacccgaag gcgcccgacg
     1741 tgatctcgtg ccgctcgtgc cacgcgcagg agggcgccaa gccgaaggtc gcctccgccg
     1801 gccccgacat cccgaaggtg acgtacgagg gcgacaccga cgccaccgaa ctcgacaacg
     1861 aagaggcgta ccaccgcaac tgcaacacgt gccacgacct cgccgtcgag gcccgcaagc
     1921 agctcaagat tccgacctcg caagagtgcg ccaagtgcca ctccggcaaa aagtaactcc
     1981 gcattcagct cgatgcgcgc gggccgcccg gccatgtgaa cggcagacgg gcgcccgcgc
     2041 tcttcgttat tcccaaacct tcccgaggag tactaacccg aaaatgcgtt acctctcttt
     2101 cgcgcccgtg ctggccctct gccttctcta cgcgcagtgc ggcggcagcc cgcccgccga
     2161 caacgcgagg cgaaactcga acgccgcccc cgccgccaac acggcgcccg ccgccaacac
     2221 gtccgcgccc cccgtcgctt cgtcgcacgg cggcggcggt acggcgaact cagggggcgc
     2281 cgcgccgggc ggagcggcgg cgccggacaa gccgccggtc gccacgccgg aactcgacga
     2341 gaagatcgag aaggccgagg cgaaggccaa gaccgcaggc gccgccgccg ccgacaagca
     2401 ggccgccgcc gccgcacaca tggcccgcgg caacttctac tacaacgcgc aggacaagcg
     2461 cctctaccgc tacgccctcg gcgacttccg ccgcgcgctc cgctacgagc cggacaacgc
     2521 cgaggccaaa gagaagatcg agatgatcga atccatctac cgctcgctcg gcaggcccgt
     2581 ccccaccaac ggcctcgaac cgtaagcgcg gagacatcgc acacgcaaaa gggcggcgcg
     2641 ttgatatcgc atcaacgcgc cgcctctttc ttgacggctc gccccggcgg gcagccgccg
     2701 ctcaacaggc gctcgtgtcg tggcacccgg cggcgtacac gagcgccagc acgcccatga
     2761 gggcgcccat gccgatgccg atcttcgcgc ccagcggaag gccgcccggc ttattgaccc
     2821 gcgccacgtc gccgtaagag agcgtccggg tctgtccgct cctggagtcc gtcagcgtga
     2881 aggaatcgtt gccggcctgg ctgacatagc ccttcagctt cgagccgtcc tgtagcctga
     2941 cgaccacgcg cgccttcttg ccggtgcccc gtcgggtgac atcggccttg accttcgcgg
     3001 cctgctgttc gctttgagcc gttcgggcga gggccgggag cggaaacacg tcggcggcgt
     3061 gaagcagcag cgccgctatg ctgagcgata cgagtctctt caacatagtc cttctcctct
     3121 tgaggcggca cgcgagggtt acgcgaggag tttcgccgat gcgggcgcga gaggggccgc
     3181 atcgccctct gcggacgagg cggcccctct cgcttcggaa gtccggggca gaaaatggac
     3241 aaaccgccgc gaccggggcg gcattgaaag acgcgtaggg acgatgcgtc aaccctcgct
     3301 actcgtcctt gatggcggcc ttcgctttct cggccagctc cggcggcact tcgtccagtc
     3361 cgtgggcggt gcgggcgtgc tcggcgacct gccccatcac ctcgtcatcc gtttcgccgc
     3421 gggccacgaa atcgcaatcc ggcccgacat ctctgcaatt catggttctc gccatcgtct
     3481 ctccttcgca aaaaagaaat gaagttgtct gactccggcg cgagaccgac agaccgtccg
     3541 cacggctcgc cgcgcggggc cgcgtcagcc gatcttgacg ccgatggatt cgaggaaccg
     3601 ggtcggcttg tcgccgccga tgagggcgaa gatgaagtcg ttggggacgg tcgccttgac
     3661 ctcctctgtg cgcgcgttca tcagcacgac ttcgccgtcg tggatctctt tgatcgcggt
     3721 gccgaagtag accttgatct tgccctcgtc gatgcagtcg tagacctgct gcttgttgcc
     3781 gaatttgagg tcgttcttca tgtcgctgcg gatgacgagc gtgacctcgt tgatctcttc
     3841 gtcggggcgg aaggtgatcg cggtgccctt gcggcgggcg accagatcga ccgctgcctc
     3901 gatggcggag ttgcccgcgc cgacgacgat catcttgcgg cggcggtact cctccgggtc
     3961 ggtcagcttg tactttacct tgtcctcggt ctcgccgtcg cgcgtgacct tcatctcctc
     4021 gccgggcacc ttgagcttca tcggggtgcc gcggttgccg agcgcgagga cgacgcgccg
     4081 cgccttgtac gtgaccttct cctttgtctc gccgccctcg ttcttgatgg tcttctcggt
     4141 ctggatgacg aagtagtcgc cgtcctcggc cttcttgacg gacttgcagc tctcggtctc
     4201 gttgatgacg acctcgctct tccgcatcgt gccgatccag tcggacagga tttcctcgcg
     4261 ctggcggccc gcgctgttca cgtcgatggc gccgcgcgac tccatcgtct cgggcttgaa
     4321 gaagacgtac ttgttggccg ggtaggcgtc gatggtggcg agcaccttgt cctgctcgat
     4381 gccgacgtaa gagagctggt gctgcttggc ggtgatggcg gcggacagtc ccgccgggcc
     4441 gatgccgatg atcgccacgt cgagttcggc cttcggctcg ggctgcgcgc cgttgttgcg
     4501 cagctcgtgc gagatgtact tgaccacgtc ggcgccctcg ttggcggcgt tcttgattag
     4561 gggcgtgccc gacacgtcgc cgatgatgaa gcagccgggc acgtccgtca tgaacttggc
     4621 gtcgcggttg gggacggggc ggggcttgat cttcttggtc gtgttgacga cgatgcacgc
     4681 cttcgggttg acggggcact cgacctggca ggaggtgtcc tccatgcact ggtcgggcgc
     4741 gatgggcgag gagatgttat cgacgatggc gagcacgtcg tgcgggcagg catcgacgca
     4801 ggcgtggcag ccgatgcagc ggtctttgat gatgacgggg tgcgggtagt ccggcccgtc
     4861 cgggtcgtac atccgccgct gctcctcggt aatcagctcg gcctgccgct ccttcttctt
     4921 ctcctgcggc ggctcttcga ggagcaggcg ggcgcgccgc gagtcgcgca gcgcgaagcc
     4981 gatggcgatg acgaggagga agagcgcgat ggccgcccag ccccaccacg gcagcccgcc
     5041 gaagaacttc ttggtcgggt cttgcgaata ctggttgacc gcgacgagca gggtcagcag
     5101 cgcgagcgtg acgaagagtt gtgccttttt catcgtaaga agttagccac agaaattcag
     5161 acggggagac gggaagacgg ggtgacgggg agcggagccg cggtagcgca agcttcgccc
     5221 gctacttttt ctccccgtct ccccatcttc tttatcaagc ctttctcatt ctctggccag
     5281 caggtgcgcg ccccacgtgc ggcgcccttg caagtgctgg acgtggcacg agtcgcagtg
     5341 cg
//
LOCUS       DBNGOH010001168         2721 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_5128424, whole genome shotgun sequence.
ACCESSION   DBNGOH010001168 DBNGOH010000000
VERSION     DBNGOH010001168.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2721)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2721)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2721
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_5128424"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..657)
                     /locus_tag="AC159P_22620"
     CDS             complement(<1..657)
                     /locus_tag="AC159P_22620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HIR3698295.1"
                     /translation="MASVGTELRDESRAKDSFLNRFLGFIERAGNILPHPATLFVILA
                     LLVVVLSAITAAMGVSVIHPGTKEVIKAESLLSVAGLHRVLSSLVTNFTGFAPLGTVL
                     VAMLGIAVAEGSGLISAVLRAAVLSAPRRLLTVVVVFAGVLSHMASDVGYVLVIPLGA
                     MIFLAVGRHPIAGLAAAFAGVSGGFCANFLLGPTDPLLAGLTQEAARILDKAYVVTPA
                     A"
     gene            complement(850..2670)
                     /locus_tag="AC159P_22625"
     CDS             complement(850..2670)
                     /locus_tag="AC159P_22625"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015094.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC1 kinase family protein"
                     /protein_id="HIR3698296.1"
                     /translation="MAFIEQEELTERAATGAAENHRANGHGTNGDRGAEASTALVVTN
                     GHGRRAGANSGHGHAHARVLPPAPATPRPLAAKESPETVALGDEDAGGLAGWWRTFQV
                     VRVLGTMSLYLFLNDYDVRARFNRKVAERKLEEARERGEKEHARARRRDLLRRLLDRL
                     IRLVRRLVFRGEEGSAGKEARLEKQAVWLKEHLIALGPTFIKIGQSLGTRADLLPLAY
                     IKELTTLQDQVPAFPTAEAYARIESELGRTLTDAFAEIDAEPIASASLGQVYRARLHT
                     GEEVAVKVQRPRLRETVALDVAVLARITRALARFPQVNRNADWEGMLREFKQTIAEEM
                     DYALEGRHADRFRHNFHDWRAVRVPHIHWSHTTSRVLTMEFIRGTKVTDIEALRARRI
                     SPVKVNRLLVRAYLKQLLEDGFFHADPHPGNLVVMDTGHLAFFDFGMTGRITPDLQSR
                     MIDSFFHVVGKDVEGLAQDLINLNFLKPGVDPNLVRPVVEGLFRHYLNLKLGEINFKE
                     LTYDLADVMYDYPFRLPSNFTYIIRALMTLEGIGIVTDPGFNFFDTAKPYAKEFMLRR
                     EGKQFRRLLLDKLTGRDEGRIDWGKMWKLAKMAARMYMEK"
BASE COUNT          437 a          851 c          971 g          462 t
ORIGIN      
        1 ggccgcaggc gtcacgacgt aagccttgtc gaggatgcgc gccgcctcct gcgtcaagcc
       61 cgcgagcagc gggtcggtcg gcccgagcag gaagttcgcg cagaagccgc ccgacacgcc
      121 cgcgaacgcc gccgcgaggc ccgctatcgg gtggcgcccc acggccagaa agatcatcgc
      181 gcccagaggg atcaccagca cgtagcccac gtcgctcgcc atgtgcgaga gcacgcccgc
      241 gaagacgacg accaccgtca ggagccgccg cggcgcggac aacaccgccg cccgcagcac
      301 cgccgagatc aagccgctcc cctcggccac cgcgatgccg agcatcgcca cgagtaccgt
      361 ccccagcggc gcgaaccccg tgaagttcgt caccagcgag ctgaggacgc ggtgcagccc
      421 cgcgaccgac agcaggctct cggccttgat gacttctttg gtgccggggt ggatgaccga
      481 gacgcccatc gccgccgtga tggccgagag cacgaccacg agcagcgcga ggatgacgaa
      541 cagcgtcgcc gggtgcggca ggatgttccc cgcccgctcg atgaacccga ggaagcggtt
      601 caggaaagag tccttcgcgc gcgactcgtc gcgcagctcg gttccgactg atgccattgt
      661 tctgcctctt cttgccgcac cctcgcgcgg cgggatggat gaacggtcgc ccctgtgatg
      721 aagtccgtaa aagcgggggt tcgctagtcg gtgaataata gacgtgtctc acagactcgt
      781 aaagtcgaaa agccttccgc gcgttggttg cgcggaaggc ttttcgacag agcgggtcat
      841 ggccgggcgt tatttttcca tgtacatcct ggcggccatc ttggcgagct tccacatctt
      901 gccccagtcg atgcggccct cgtcgcggcc cgtgagcttg tcgagcagga ggcggcggaa
      961 ctgcttgcct tcgcggcgga gcatgaactc tttggcgtaa ggcttggccg tgtcgaagaa
     1021 gttgaagccg gggtcggtga cgatgccgat gccttcgagc gtcatcagcg cgcggatgat
     1081 ataggtgaag ttcgacggca ggcggaacgg gtagtcgtac atcacgtccg ccaggtcgta
     1141 ggtcagctcc ttgaagttga tctcgccgag cttgaggttg agatagtggc ggaacaatcc
     1201 ctcgaccacg ggccggacga ggttcgggtc aacgccgggc ttgaggaagt tgaggttgat
     1261 caagtcctgc gccaagcctt ccacgtcctt gccgacgacg tggaagaagg agtcgatcat
     1321 ccgcgactga aggtcgggcg tgatgcggcc cgtcatgccg aagtcgaaga aggcgaggtg
     1381 gcccgtgtcc atcacgacga ggttgccggg gtgcgggtcg gcgtggaaga agccgtcttc
     1441 caggagctgt ttcaggtacg cgcggacgag caggcggttg accttgacgg gcgagatgcg
     1501 gcgcgcgcgc agggcctcga tgtccgtcac cttcgtgccg cggatgaact ccatcgtgag
     1561 cacgcgcgag gtggtgtgcg accagtggat gtggggcacg cgcacggcgc gccagtcgtg
     1621 gaagttgtgg cggaagcggt cggcgtggcg gccttcgagc gcgtagtcca tctcctcggc
     1681 gatggtctgc ttgaactcgc gcagcatccc ctcccagtcg gcgttgcggt tcacttgggg
     1741 gaagcgcgcg agcgcgcgcg tgatgcgggc gagcacggcc acgtcgagcg cgacggtctc
     1801 gcgcaagcgc gggcgctgga ctttgacggc gacctcttcg cccgtgtgca gccgcgcgcg
     1861 gtagacctgc ccgagcgagg ccgaggcgat aggctcggcg tcgatctcgg cgaaggcatc
     1921 ggtcagcgtg cgaccgagtt cggactcgat gcgggcgtag gcttcggcgg tggggaacgc
     1981 gggcacctga tcctggagcg tcgtgagttc cttgatgtag gcgagcggca aaaggtctgc
     2041 gcgcgtgccg agcgactggc cgatcttgat gaaggtcggc ccgagcgcga tgaggtgctc
     2101 cttgagccag acggcctgct tttcgagccg cgcctcttta ccggcggagc cttcctcgcc
     2161 gcggaagacg aggcggcgga cgaggcggat gaggcggtcg agcaggcggc ggagaagatc
     2221 gcgccggcgc gcgcgcgcgt gctccttctc gccgcgctcg cgtgcctctt cgagcttgcg
     2281 ctcggccacc ttgcggttga acctggcgcg cacgtcgtag tcgttcagga aaaggtagag
     2341 cgacatcgtg ccgagcacgc gcacgacctg gaacgtgcgc caccagccgg cgaggccgcc
     2401 cgcgtcctcg tcgccgagcg cgacggtctc cggactctcc ttcgcggcga gaggccgcgg
     2461 cgtcgcgggc gccggaggga gcacgcgcgc gtgggcgtgg ccgtggccgc tgttcgcacc
     2521 ggcgcggcgt ccgtggccgt tcgtcacgac gagggcggtc gaagcctccg cgcctcggtc
     2581 gccgttggtg ccgtggccgt tggcgcggtg attctcggcg gcgcccgtcg cggctcgttc
     2641 cgtcaactct tcctgttcga tgaaagccat gctgtctgga atgataactg tcggattttt
     2701 caatcgtgtc tcgattatga g
//
LOCUS       DBNGOH010001169         3792 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2194620, whole genome shotgun sequence.
ACCESSION   DBNGOH010001169 DBNGOH010000000
VERSION     DBNGOH010001169.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3792)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3792)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3792
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2194620"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1209)
                     /locus_tag="AC159P_22630"
     CDS             complement(<1..1209)
                     /locus_tag="AC159P_22630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011388079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HIR3698297.1"
                     /translation="MPIQLDGTPSGLAFTSRQTVLIHRLDLADFPAPLIKRTFEDGVR
                     SGCSVPLISHDRVLGTITVGAASEAAYSAEDAELLAEVAQQIAMPVENVLNFRRAERE
                     RDRARLVLEASNAIVSNLDLRDLLMSTSACLRRYFKHDFLGLSLYDEEIGQLRLHALD
                     TLPDGRFIEDGTPLPMEGTPAGLAFSSRLPVLRQRLSVEEFPSPIVKMAWESGLRSGC
                     TIPLISHERALGVIAVASRREAAFTPEDVELLQHIANEVAIAVENVLLYREIASLKNK
                     LASEKHYLEEEIQTEYNFAEIVGQSRALRRVLEQVETVAPTDSVVLICGETGTGKELI
                     ARAIHSLSARRERTLVKLNCAAIPTGLLESELFGHEKGTFTGAVAQRIGRFELAHKGT
                     LLLDEIGEIPL"
     gene            complement(1146..1487)
                     /locus_tag="AC159P_22635"
     CDS             complement(1146..1487)
                     /locus_tag="AC159P_22635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3698298.1"
                     /translation="MNHELKPLSRSPASAAERQPSKGDPDRSRLLLEINNAVVSELDL
                     EGLLKAIFDCLRQIFRQTIAATISIYDPESNLLRVHLLHAPQPKPSARGCPSNSTGRR
                     AASPSPRARPS"
     gene            complement(1722..>3792)
                     /locus_tag="AC159P_22640"
     CDS             complement(1722..>3792)
                     /locus_tag="AC159P_22640"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HIR3698299.1"
                     /translation="QGGVRRGANMGIMRVDHPDLLRFIHAKNDQHSLTNFNISVTVTD
                     GFFKAVDDGEWYQLSFDGTPWDEPVFDPVQEDNYAVYRRPDGTTVTFRDRAAFLEAES
                     SGLLARCTREEPPRPGMVYAPDVWNRVVASAHRYAEPGVIFIDEVNRHNHMIGSMGPI
                     YATNPCAEQALHFNNSCNLGSIDLAKFYDADTKVDWQRLAETTRWCVRFLDNVIDACT
                     WPLPEIDDVVKRTRPVGLGVMGFADLCIQLKVKYGSPASIDLMDEVMSFVRREAWTES
                     CLLGSEKGVFPEYHPNRESYDRFLREDIGVAADVPLTPRNYETTTIAPTGTISLVAET
                     SSGVEPNFSWAYVRQDTLGKRTYVHTLAAEALGMDVDQTDQESIDAAAAYVVEHEAEL
                     PDYFISAMSITAEQHVYVLAAAQRHVDNGVSKTCNGAHDDTVESVDRLYRLARELGCK
                     AVSYYRDGSRESQVLTSVKGAEAKGEIACDPAITTEATASEAAIDEDVVEAGDINQTV
                     TTPPPAPRPAAPTSSTTRIERPRELKGATWQIPFDGQNLYVTVNHDGREILEVFATGP
                     ISGGVGLLASKMLRGGFDAREVARSLNKVTGTHSVWFNERLLTSPEQAVAECVMLTSR
                     RIQNQPDSERALAYQQPVISVSVPDDARPISGNYALISECPECNGQLEHASGCDFCRD
                     CGYSKCK"
BASE COUNT          589 a         1180 c         1338 g          685 t
ORIGIN      
        1 gagaggtatc tcgccgatct cgtccaagag gagcgtgccc ttgtgggcca gctcgaagcg
       61 cccgatgcgc tgcgcgacgg cgccggtgaa agtgcccttc tcgtggccga acagctcgga
      121 ttcgagcagc cccgtgggga tggccgcgca gttgagcttg acgagcgtgc gctcgcgccg
      181 cgcgctcaag gagtggatgg cgcgggcgat cagctccttg ccggtgccgg tttcgccgca
      241 gatgaggacg accgagtcgg tcggcgccac ggtctcgacc tgttcgagca cgcgcctgag
      301 cgcgcggctc tggccgacga tctcggcgaa gttgtattcg gtctgaatct cctcctcaag
      361 ataatgtttc tcgctcgcga gcttgttctt gagcgacgcg atctcgcggt acaggaggac
      421 gttctcgacc gcgatggcga cctcgttggc gatgtgctgg agcagttcca cgtcttcggg
      481 cgtgaaggcc gcctcgcggc ggctcgccac ggcgatcacg ccgagcgccc gctcgtgcga
      541 gataagcggg atcgtgcaac ccgaccgcag gccggactcc cacgccatct tcacgatcgg
      601 cgaaggaaac tcctccacgc tcaagcgttg ccgcaagacg ggcaggcgcg aagaaaaggc
      661 caacccggcg ggcgtccctt ccatcggcag cggcgtaccg tcttcgatga acctgccgtc
      721 gggcagcgtg tcgagcgcgt gcagccgcaa ctgcccgatc tcttcgtcgt agagcgagag
      781 accgaggaag tcgtgtttga aataccggcg caggcacgcg gacgttgaca tcaaaaggtc
      841 gcgcaggtcg aggttcgaga cgatggcgtt gctcgcttcg agcaccagcc gcgcgcggtc
      901 gcgctcgcgc tcggcgcggc ggaagttcag cacgttctcg accggcatgg ctatctgttg
      961 tgcgacctct gcgagcagtt cggcgtcctc cgcggagtac gcggcctcgc tcgcggcgcc
     1021 gacggtgatg gtgccgagca cgcggtcgtg ggagatgagc gggacgctgc aaccggagcg
     1081 gacgccgtcc tcgaaagtgc gtttgatgag cggcgcgggg aagtccgcca agtccagacg
     1141 gtgaatcaag acggtctggc gcgaggtgaa ggcgaggccg ctcggcgtcc cgtcgagttg
     1201 gatgggcatc ccctcgcgga aggtttcggc tgcggggcgt ggagcaggtg aacccgtaac
     1261 aggttacttt ccgggtcgta gatggagatg gtcgcggcaa tcgtctgtcg gaaaatctgt
     1321 cggagacagt cgaagatggc cttgagcaag ccttcgaggt cgagttcgga gaccaccgcg
     1381 ttgttgatct ccagcaacag ccgcgaccga tccgggtcgc ctttcgaagg ctgccgctcg
     1441 gcggctgacg ccgggcttct actcaggggt ttcagttcgt gattcatcgc cgttggggcg
     1501 gcggttcttt gcgtgcgagg gaaatcgcct cttccgtcct ctacgatgtc gcgcgcggtg
     1561 tgaacggatt atagcgagaa gggccggagc cgtacagagt gaccgggaac gacaaaaagg
     1621 caagccccga atctcttcgc ggagattcgg ggcttgcctt ttgtttctac cgacgactca
     1681 gcctgcgccg ggcgggagcg aagcggacgc ttcgaggtcg ctcacttaca cttcgagtag
     1741 ccgcagtcgc ggcagaagtc gcagcccgag gcgtgttcga gctggccgtt gcattcgggg
     1801 cactcgctga tgagcgcgta gttgccgctg atgggccggg cgtcgtcggg gacgctgacg
     1861 gagatgacgg gctgctggta ggcgagcgcg cgctccgagt cgggctgatt ctggatgcgg
     1921 cggctcgtga gcatgacgca ctcggcgacg gcctgttcgg gcgaggtcag cagccgctcg
     1981 ttgaaccaga ccgagtgcgt gcccgtcacc ttgttgagac tgcgcgccac ctcgcgcgcg
     2041 tcgaagccgc cgcgcaacat cttcgaggcc agcaggccca ccccgcccga gatcgggccg
     2101 gtggcgaaga cttcgagaat ctcgcgcccg tcgtggttga ccgtgacgta gaggttctgc
     2161 ccgtcgaacg ggatctgcca cgtcgcgccc ttcagctcgc gcgggcgctc gatgcgcgtc
     2221 gtgctcgaag tcggggcggc ggggcgcggt gcgggcggag gggtcgtcac cgtttgattg
     2281 atgtccccgg cctcgacgac atcttcgtcg atcgccgcct cgctcgcggt cgcttcggtc
     2341 gtgatggccg ggtcgcaggc gatttcgccc ttggcctccg cgcccttcac gctcgtcaac
     2401 acctgcgatt cgcggctccc atcgcggtag tagctgacgg ccttgcagcc cagctcgcgc
     2461 gccagacggt agagcctgtc caccgactcg accgtgtcgt cgtgcgcgcc gttgcaggtc
     2521 ttcgacacgc cgttatcgac gtggcgctgc gcggcggcca gcacgtagac gtgctgctcg
     2581 gcggtgatcg acatcgccga gatgaagtag tccggaagct ccgcctcgtg ctcgacgacg
     2641 taagcagccg ccgcgtcgat cgactcctga tcggtctggt caacgtccat gccgagcgcc
     2701 tcggcggcga gcgtgtggac gtaggtgcgt ttgccgagcg tgtcctgccg gacgtaggcc
     2761 cacgagaagt tcggctcgac gcccgaggag gtctccgcga cgagcgagat ggtgccggtc
     2821 ggcgcgatgg tcgtcgtctc gtagttgcgc ggcgtcagcg gcacgtccgc cgccaccccg
     2881 atgtcttcgc gcaggaagcg gtcgtaagat tccctgttcg ggtggtactc ggggaagacg
     2941 cccttctccg agcctaagag gcagctctcc gtccaggcct cgcgccgcac gaacgacatc
     3001 acctcgtcca tcaggtcgat cgaggcgggc gagccgtact tcaccttgag ctggatgcac
     3061 aggtctgcaa agcccatcac gccgaggccc acggggcgcg tccgcttcac cacgtcgtcg
     3121 atctcgggga gcggccacgt gcaggcgtcg atcacgttgt cgaggaagcg cacgcaccag
     3181 cgcgtcgtct cggcgaggcg ctgccagtca accttcgtgt cggcgtcgta gaacttggcg
     3241 aggtcgatcg agccgaggtt gcacgagttg ttgaagtgga gcgcctgctc ggcgcagggg
     3301 ttcgtcgcgt agatcggccc catgctgccg atcatgtggt tgtggcggtt gacctcgtcg
     3361 atgaagatga cgcccggctc ggcgtagcgg tgggccgagg cgacgacgcg gttccacacg
     3421 tcgggcgcgt agaccatccc ggggcgcggc ggctcctcgc gcgtgcagcg cgcgagcagg
     3481 cccgagcttt cggcttcgag gaaggcggcg cggtcgcgga aggtgacggt cgtgccgtcc
     3541 gggcggcgat agacggcgta attgtcttcc tgcacggggt cgaagacagg ctcgtcccag
     3601 ggcgtgccgt cgaaggagag ctgataccac tcgccgtcgt ccacggcctt gaagaacccg
     3661 tccgtgaccg tcaccgagat gttgaagttg gtcaggctgt gctggtcgtt cttcgcgtgg
     3721 atgaagcgga gcaggtcggg gtgatcgacg cgcatgatgc ccatgttcgc gccgcggcgc
     3781 acgccgccct gc
//
LOCUS       DBNGOH010001170         8317 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2928860, whole genome shotgun sequence.
ACCESSION   DBNGOH010001170 DBNGOH010000000
VERSION     DBNGOH010001170.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8317)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8317)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8317
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2928860"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..463)
                     /locus_tag="AC159P_22645"
     CDS             complement(<1..463)
                     /locus_tag="AC159P_22645"
                     /inference="COORDINATES: protein motif:HMM:NF019095.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HIR3698300.1"
                     /translation="MKKVLVAASLVISAAVLVTGFTQGFKSIKEFLTGYEEVPAVSTV
                     AGAVFTARISDDETSLEYELSYADLEGAVQQAHIHFGQRGVNGGISVWLCGNASAGPP
                     PINPPAGTPACPPSPATVTGTLTAANVVGPAGQGIAAGEFAELVAAIRAGKT"
     gene            complement(513..2981)
                     /locus_tag="AC159P_22650"
     CDS             complement(513..2981)
                     /locus_tag="AC159P_22650"
                     /inference="COORDINATES: protein motif:HMM:NF020683.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose oxidase-like domain-containing
                     protein"
                     /protein_id="HIR3698301.1"
                     /translation="MRDNSFYTNARRVTAGRLFAAGRALRARRRYLAGVALLACLIAF
                     GLSSGRPDRIASAQATFPDPNANCPRAQCGEVSPLIPMQSAEAVHMGLVWKRSSPTPK
                     ILFHSRFPEYTPNDVADPALIDLAIARGAFTTPGLQFNSSLRDVLHGFDPFLGLGTNR
                     SPDDSFQELTYGGYLLRQGLSQSVPTRIVANRTMERQLLFDIAHPDAFKNTGKFHTAL
                     LDEADFTLNRAAFAENGYSKGMFYNTYCNARVTLSDGRVYVFGGHDMQSNNGLYKVNI
                     FDPETETWVRRREPCTRANWARDPFGLQLFAQNPNAQFYPDCDPRDQQSTQPSEPSDQ
                     KYSRWYPSAIPLPDDTVLVVGGFDQDNTVGPDPLRAAKGIQNQSQNDSAFTASRVNIV
                     VPEVYDPKTDRNIALENARMAFPLYPQLEVVQTGPGRDDWMVCTFNGEIDFGVERIPG
                     GARFGVGGGTPPFTKGTTWCLDVLGALRDPGRNTPAKNHWRLIDTALEVRPYCCPTAS
                     LVEIDGDGHTVSHKWFMISGQNADGDQTGTVEEIEFTDASPRWRKVGDILQPLSTTKA
                     VLLPDGKVLIGHGVNRAGNCTVTEGGVTRPCTFEEREGHHFQMFDPADGSITKLGKST
                     VSRGLHGTASLLPDGSVFFAGENREALIRPDDPSFPLLTSYAGLLPRGDPDQGVPVGQ
                     LFFPPYLFNVDGSRAARPVIKKSPEEIAYRGHFDITVGGDPREIASVGLLRSDHNTHS
                     LTAGDRYVKLAFHQKGAARKGELRVRAPKLPAQAIPGIYMLYVVDGAGVPSIAQRVYL
                     KPEEQGRRAVERGAAHGGLKAHSR"
     gene            complement(3221..4048)
                     /locus_tag="AC159P_22655"
     CDS             complement(3221..4048)
                     /locus_tag="AC159P_22655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR3698302.1"
                     /translation="MEQRLPSGHLYGEMLGRREAAGLVLMENAYSPGLRLPRHSHEHA
                     GFCFVLRGTFDRVYERKTWACGPSSLSFHPSDERHSERFEGAGARLFSIQISQRLLER
                     ARDYSIALGDSAVFRGGLPAQLALRLYGEYRRMDDVSPLAIEGLALEMMAEASRGSAG
                     SAKRRSPAPRWLRQAVDLLHAQFSETPTLSAVALSVGVHPVHLARTFRRHYRCTVGDY
                     IRRLRVESACRTLSTSDTPLAEVALLAGFSSQSHFSTAFKLHTGMTPAEYRSAFRPR"
     gene            complement(4265..5599)
                     /locus_tag="AC159P_22660"
     CDS             complement(4265..5599)
                     /locus_tag="AC159P_22660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3698303.1"
                     /translation="MNLRSTTRRVEEDGAGGGRDWLLCRGVKSLLRCDEAEVVEGIAR
                     HIMSQHGSLRIGSALMERARGTPLPAAAALLGGLTRFLEVRDGACKDSAVWIARLGNE
                     RRAVESLPPSLSELGWVEWKFRRRPAAAALAASLRGVASVRRVFTVARRLHRRREFFK
                     VLRVVELVGYYARYLGLFRGGRYRLALMSNHSNPHGIAFNLAARKCGVPVVLVTHGMP
                     VRPVARLGYDLAVVHCEAARRTYLEAGCRIERVLVHGRRQDHAPMPPGPLPERLSVGV
                     FLCKDVNEGRLRTLVERLLADVRVARVLVRPHPKNLWVGLDAWIESRSEPRLRRCPAG
                     PVAQDLEAADIVLAGNSSVLVDAVTAGRPAGYVSGIDHGPTDLHEFVARGLIFPIEER
                     LDLDPDAMLRFYRQPDWPTVLRLFANVDEDEAAVMEQVREVMRSLAGIQVFG"
     gene            complement(5638..6579)
                     /locus_tag="AC159P_22665"
     CDS             complement(5638..6579)
                     /locus_tag="AC159P_22665"
                     /inference="COORDINATES: protein motif:HMM:NF012607.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HIR3698304.1"
                     /translation="MKWRVLISAPYMLPVVEDLRERLEAEGVEVVKARVRERLSEAEL
                     LPLVGTIDGAICGDDQFTERVLRAAPRLKVISKWGTGIDSIDARAAARLGIRVCNTPD
                     AFTDAVADTALGYVLCFARRLPSMDAHVRRGRWLKPDAVSLGECTLGVVGVGNIGRAV
                     VCRARAFGMTALGTDPRRVPAAFVEETGLRMLPLRALLEEADFVSLHCDLNPTSFHLI
                     GRAELAAMRPTAYLINTARGPVEDEPALARALGERRIAGAALDVFEVEPLPADSPLRA
                     LDNCLLAPHNANSSPAARRRVHESTIANLLGALREAG"
     gene            complement(6576..7385)
                     /gene="kdsB"
                     /locus_tag="AC159P_22670"
     CDS             complement(6576..7385)
                     /gene="kdsB"
                     /locus_tag="AC159P_22670"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HIR3698305.1"
                     /translation="MALRTDVRVVAIIPARMGSSRFPGKPLAPLHGRPMIEHVVRRAV
                     MCERLDAVYVATCDDEIRTAVEAWGGAVLMTSPAHERASDRVAEAAEHVEADIVVMIQ
                     GDEPMCTPDMIDAAVAPLLEDSSVGCVNLARRIASREEYLDRNTIKVVRNVRGDALYF
                     SRSPIPALDFARDFPIGRTPVLKQVCVIPFRRDFLREFARLTPTPLERAESIDMLRVI
                     EHGGHVRLVETRAVTHAVDTPEDLRLVESLMKGDPLLTLYGEPSARARSRA"
     gene            7560..8276
                     /locus_tag="AC159P_22675"
     CDS             7560..8276
                     /locus_tag="AC159P_22675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015142615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HIR3698306.1"
                     /translation="MSRSAQPHFITKNRLLAALPRQEYERLAPHLEPMHLPRGKTIAL
                     TGDTLRYAYFPTSGMLSLLSTSGGGEAIEVAMVGSEGFVGVPIILKVGVTPYWSMVQI
                     QSDAMRISAAALGEEFDRCEQLHDLLLKYTHSLLAHVTQSAVCNRFHTVQERLCRWLL
                     LSRDRMKSDTINLTQEFISQMLGTARTGVTMAAVPLQDAGLIRYRRGKITILDREGLE
                     SVACECYSIVRKEVSHTLAA"
BASE COUNT         1369 a         2768 c         2811 g         1369 t
ORIGIN      
        1 acgtcttccc ggcgcggatc gccgcgacca attcggcgaa ctcgcccgcc gcgatgccct
       61 gccccgccgg gccgacgacg ttggctgccg tgagcgtccc cgtgacggtc gcaggcgaag
      121 ggggacaggc cggcgtgccc gccggggggt tgatcggggg cggccccgcg ctggcgttgc
      181 cgcagagcca gacgctgatg ccgccgttga cgccccgctg gccgaaatgg atgtgggcct
      241 gctggacggc gccttcgaga tcggcgtagc tcagctcgta ttcgagcgac gtctcgtcgt
      301 cgctgatccg cgcggtgaac acggcgccgg cgacggtcga gacggcgggc acttcctcgt
      361 acccggtcag aaactccttg atgctcttga acccttgggt gaaacccgtc accagtactg
      421 ccgcggaaat caccagcgag gctgccacca acactttttt catctcccac ctcctatact
      481 ttggggtcgg cccttcgaat caaagaacgt tttcaacggc tgtgtgcttt gagaccgccg
      541 tgtgcggcac cgcgctccac ggcgcggcgg ccctgctctt cgggcttcag gtagacgcgc
      601 tgcgcgatgc tcgggacacc cgccccgtca acgacgtaga gcatgtagat tcccggaata
      661 gcctgcgcgg gcagcttcgg cgcacgcaca cgcagttccc ctttgcgggc cgctcccttt
      721 tggtgaaacg ccagcttcac atagcggtca ccggcggtca aactgtgtgt gttgtggtcg
      781 ctgcgcagta gcccgacgga ggcgatctcg cgcgggtcgc ccccgaccgt gatgtcgaag
      841 tggccacggt aggcgatctc ctcgggcgac ttcttgatga cgggtcgcgc cgcgcggctg
      901 ccgtccacgt tgaagagata cggcgggaag aagagttgcc cgacggggac gccttggtcc
      961 gggtcgccgc gcgggagcag gccggcgtac gaggtcagca gcgggaacga agggtcgtcg
     1021 ggccggatga gcgcctcgcg gttctcgccc gcgaagaaga ccgacccgtc cggcagcagc
     1081 gacgccgtcc cgtggagtcc gcgcgagacc gtgctcttcc ccagcttcgt gatcgagccg
     1141 tccgcggggt cgaacatctg gaagtgatgg ccttccctct cctcgaaggt gcacggcctt
     1201 gtcacgccgc cctccgtgac ggtgcagttg cccgcccggt tcaccccgtg gccgatgagc
     1261 accttgccgt cggggagcag cacggccttc gtcgtcgaga gaggttgcag gatgtctccg
     1321 accttgcgcc accgcgggct ggcgtcggtg aactcgatct cctcgacggt gccggtctga
     1381 tcgccgtccg cgttctgccc cgagatcatg aaccacttgt gcgacacggt gtgcccgtcg
     1441 ccgtcgatct cgacgaggct cgcggtcggg caacagtagg gcctgacttc gagggccgtg
     1501 tcgataagcc tccagtggtt cttcgcgggc gtgttccgtc cggggtctct caacgccccg
     1561 agcacgtcca ggcaccaggt cgtgcccttc gtgaaggggg gcgtgccccc gccgacgccg
     1621 aaccgtgcgc cgcccggaat ccgctcgacg ccgaaatcga tctcgccgtt gaaggtgcag
     1681 accatccagt cgtctctgcc cggcccggtc tggacgactt cgagctgcgg gtagagcggg
     1741 aaggccatgc gtgcgttctc cagcgcgatg ttgcggtcgg tcttcgggtc gtacacttcg
     1801 ggcaccacga tgttgacccg cgaggccgtg aaggccgaat cgttctggct ctggttctgt
     1861 atgcccttcg cggcccgcaa ggggtctggc cccaccgtgt tgtcctgatc gaatccgccg
     1921 acgacgagca ccgtgtcatc gggcagcgga atcgccgacg gataccagcg cgagtacttc
     1981 tggtcggaag gctcggaggg ctgcgtgctc tgctggtctc tggggtcgca gtcggggtag
     2041 aactgcgcgt tcgggttctg ggcgaacagt tgcaggccga acgggtctct ggcccaattc
     2101 gcgcgggtgc aaggctcgcg acggcgcacc catgtttccg tctccgggtc gaagatgttg
     2161 accttgtaca gcccgttgtt gctctgcatg tcgtggccgc cgaagacgta gacccgcccg
     2221 tcggacaacg tcacgcgcgc gttgcagtac gtgttgtaga acatcccctt cgagtagccg
     2281 ttctcggcga acgccgcacg gttgagggtg aagtcggcct cgtcgagcag ggccgtgtgg
     2341 aacttgcccg tgttcttgaa ggcgtcgggg tgggcgatgt cgaagagcag ttggcgctcc
     2401 atcgtccggt tggcgacgat gcgcgtcggc acgctctggc tgaggccctg ccgcaacagg
     2461 tagccgccgt aggtgagttc ctggaaagag tcgtcgggcg agcggttcgt cccgaggccg
     2521 aggaacgggt cgaagccgtg gaggacatcc ctcagcgaag agttgaactg gagaccgggg
     2581 gtcgtaaacg ccccgcgcgc gatggcgagg tcgatcagcg cggggtcggc cacgtcgttc
     2641 ggtgtgtact ccgggaaccg cgagtggaag aggatcttgg gcgtcggcga actcctcttc
     2701 cacaccagcc ccatgtgcac ggcctccgcg ctctgcatcg ggatcagtgg cgacacctcg
     2761 ccgcactgcg cgcgcgggca gttggcgttg gggtccggga atgtggcctg tgcggatgca
     2821 atccggtcgg gcctgcctga tgacaggccg aaggctatca ggcacgcgag cagcgcgacg
     2881 cccgcgaggt aacgccgccg tgcccgaagc gcgcgccccg ccgcgaacag gcgaccggct
     2941 gtcacccgtc tcgcgttcgt gtaaaacgag ttatccctca tcacgtccaa ctcctttccc
     3001 aacctgcctg tctgacagac ctgcgaacaa ttcaaccggc ggccagagcg ggccgaaccg
     3061 cgtcccgcga ccggatgctc tctttgaacg acagggggcg gacgggcttc tgacgacgag
     3121 ggcggggaga aagggcaagt tgtgctttgg gctgtgcgat gtggagttta gcaggcgggc
     3181 gggggcgggt ctttcaccac cgaaacattt ttcaacccga ttagcgcggg cggaaggcgc
     3241 tacggtactc ggcgggggtc atgcctgtgt ggagtttgaa ggccgtcgag aaatggctct
     3301 ggctggagaa gccggcgaga agggcgacct ccgcgagcgg ggtgtcggac gtggaaagtg
     3361 tgcggcaggc ggactcgacc cggagccgtc ggatgtaatc tccgaccgtg cagcggtaat
     3421 gtcggcggaa ggtgcgggct aaatgaaccg ggtggacgcc gacggagagc gcgaccgcgg
     3481 agagcgtcgg cgtctccgag aactgcgcgt ggagaagatc gaccgcctgc cggagccagc
     3541 gcggcgcggg gctgcggcgc ttcgccgacc cggcggagcc gcgcgaagcc tcggccatca
     3601 tctccagcgc gaggccctct atcgccagcg gggagacatc gtccatccgt cggtattcgc
     3661 cgtagaggcg cagtgcgagc tgggccggca ggccccctcg gaagaccgcc gaatcgccga
     3721 gcgcgatcga gtagtcgcgc gcgcgctcca gcagacgttg gcttatctga atgctgaaca
     3781 ggcgcgcgcc cgcgccctcg aaccgctcgg agtgtcgctc gtccgaaggg tggaaggaga
     3841 ggctcgacgg cccgcacgcc cacgtttttc tttcgtagac tctgtcgaac gtgccccgca
     3901 ggacgaagca aaagccggcg tgctcgtgcg agtggcgggg aagtctcaaa ccgggcgagt
     3961 aggcgttttc catcaggacg aggcccgcag cctcgcgtcg gcccagcatc tccccgtaca
     4021 agtggccgga aggaagcctc tgttccatcg agagcctcta cgtggaagcc gccccatacc
     4081 gtacagtggg gccggctcta ttaatgtcgg ggagccgcga aatcgcgatg cgctgatatc
     4141 caggttacaa accggcaaga tgtatgccgc cgcgcgcact cttcggtaag ccgttccggc
     4201 caaagttctt aacccgccga cggctgtagc gggagggtca cggacaattg ctgcgattgg
     4261 ataatcatcc gaaaacttga atgcccgcga gcgagcgcat gacttccctg acctgctcca
     4321 tgacggcggc ctcgtcctcg tccacgttgg cgaagaggcg gagcacggtc ggccagtcgg
     4381 gctgccggta gaaacggagc atcgcgtcgg gatccaagtc gagacgctcc tcgatcggga
     4441 agatcaaacc gcgcgcgacg aactcgtgga ggtctgtggg gccatggtcg atgcccgaca
     4501 cgtagcctgc cggcctgcct gcggtgacgg catcgacgag cacgctggag ttaccggcga
     4561 ggacgatgtc cgccgcctca aggtcttgcg cgacaggccc agcgggacag cgccgcaggc
     4621 gtggctcgct gcgcgattcg atccacgcgt cgagtccgac ccagaggttc ttcgggtgcg
     4681 ggcggacgag aacgcgcgcc acgcgcacgt ccgcaagcag ccgctcgaca agcgtgcgca
     4741 gccgcccctc gttcacgtcc ttgcaaagaa acaccccgac actcaaccgc tcagggagcg
     4801 gccccggagg catcggggca tgatcctgtc gccgcccgtg aacgaggacg cgctcgatgc
     4861 gacagcccgc ctcaaggtac gtccgccgcg cggcctcgca atggactacc gccagatcgt
     4921 atccgagccg cgcgacgggc cggacgggca tgccgtgtgt gacgaggacg acgggcacgc
     4981 cgcactttct cgccgccagg ttgaatgcga tgccgtgcgg gttgctgtga ttcgacatca
     5041 acgccagacg gtagcgcccg ccgcggaaga ggccgaggta acgagcgtag tagccgacga
     5101 gttcgacgac gcgcagtacc ttgaagaact cgcgccgccg gtgaagcctc cgcgcgaccg
     5161 tgaagacgcg tcgcacggag gcgacgcccc gaagcgacgc ggcgagggcg gcggcggcgg
     5221 ggcgcctgcg gaatttccat tcgacccaac cgagttccga aagcgacggg ggcaacgact
     5281 cgacggcgcg ccgttcgttg ccgagtcgcg cgatccagac cgcgctgtcc ttgcacgcgc
     5341 cgtcgcgcac ttcgagaaat ctcgtcagcc cgccgaggag ggccgccgcc gcagggagcg
     5401 gcgtcccgcg cgcgcgctcc atcaacgccg acccgatccg cagactgccg tgctgcgaca
     5461 tgatgtggcg cgcgatcccc tcgaccactt cggcctcgtc gcaccgcaag aggctcttga
     5521 cgccgcgaca gagcagccag tcgcgcccgc cgcccgcgcc gtcttcctca acgcgtcgcg
     5581 tggttgaccg aaggttcatc ggctcgcttc gattccctgc ccgcccgcgc gcactcgtca
     5641 ccccgcttct cgcaacgccc cgagcaggtt ggcgattgtt gactcgtgga ctcgacgacg
     5701 cgccgcggga ctgctgttcg cgttgtgggg cgcgagcagg caattgtcga gcgcgcggag
     5761 cgggctgtcc gccggcagcg gctcgacctc gaaaacgtcg agcgcggccc ctgcgatccg
     5821 ccgctcgccc agcgcacggg cgagagccgg ctcgtcctcc acagggccgc gcgcggtgtt
     5881 gatgaggtag gccgtgggcc gcatggccgc caactccgcg cggcctatca ggtggaacga
     5941 ggtcgggttg aggtcgcagt ggagggagac gaaatcggcc tcctccagca gcgcgcgaag
     6001 cggcagcatg cgtagtccgg tctcttcgac gaacgccgcg ggcacgcgcc gcgggtctgt
     6061 gccgagcgcc gtcatcccga aggcgcgcgc gcggcacacg acggccctgc cgatgttccc
     6121 gaccccgacg acgccgagcg tgcattcacc gagcgagacg gcgtccggct tgagccaccg
     6181 tccgcgccgg acatgcgcgt ccatcgacgg gaggcggcgc gcgaagcaga gcacgtagcc
     6241 gagcgctgtg tcggcgacgg cgtccgtgaa ggcgtcgggc gtgttgcaca cgcggatgcc
     6301 cagtcgcgcc gcggcgcgcg cgtcgatgga gtcgatgccc gtgccccatt tcgagatcac
     6361 cttcagccgc ggcgcggcac gcaacacgcg ctccgtgaac tgatcgtcgc cgcagatcgc
     6421 gccgtcgatc gtcccgacga gggggagcag ttccgcctcg ctcagccgtt cgcgcacgcg
     6481 cgccttgacg acctccacgc cttcggcctc aagccgttcg cgcaagtcct cgacgacggg
     6541 gagcatgtag ggcgcgctga tgaggactcg ccacttcatg cgcgcgacct cgcgcgcgcg
     6601 gacggctcgc cgtagagggt caggagcggg tcgcccttca tcagcgactc gacgaggcgc
     6661 aggtcttcgg gcgtgtcaac ggcgtgcgtg accgccctag tctccaccag ccgcacgtgg
     6721 ccgccgtgct cgatcacgcg gagcatgtcg atggactccg ctctttcgag cggcgtcggc
     6781 gtgaggcgcg cgaactcgcg gaggaagtcg cgccggaaag ggatcacgca cacctgttta
     6841 agaaccggcg tgcgcccaat cgggaagtcg cgcgcgaaat caagagcggg gatgggggat
     6901 cgtgagaagt agagggcgtc gccgcggacg ttacgcacga ccttgatggt gttgcgatca
     6961 agatattctt cgcggctggc gatcctgcgc gcgaggttca cacacccgac ggacgaatct
     7021 tcgagcaagg gggccacggc ggcgtcgatc atgtccgggg tgcacatcgg ctcgtccccc
     7081 tgaatcatca cgacgatgtc ggcctccacg tgctcggcgg cttcggcgac gcggtcgctc
     7141 gcccgttcgt gtgcaggcga agtcatcagg acggcgccgc cccacgcttc gacggcggtc
     7201 ctgatctcat cgtcgcaagt cgcgacgtaa accgcgtcga gccgttcgca catcacggcg
     7261 cggcggacga cgtgctcgat catcggacgc ccgtggagcg gggcgagagg cttgccgggg
     7321 aagcgtgacg aacccatccg cgccgggatg atcgcgacaa ccctgacatc ggttcggagg
     7381 gccattaatc cttccacccg cgtccgccgc gcgccgcggg ccgggccgag ttgtttgccg
     7441 actcccgcag gcgggtttat aatttcgcgc atctgccgga gtcttctcgc gacaagttgc
     7501 agttgccctg acttctcacg cgtgcccaca tcacacttct cacgaacgga ggagttctca
     7561 tgtcgcgctc cgcacagccg cattttataa caaaaaaccg actcctcgcc gccctgcccc
     7621 gtcaggagta cgaacgcctc gcgccccacc ttgagccgat gcacctcccg cgtgggaaga
     7681 ccatcgccct cacgggcgac acgcttcgct atgcctactt ccccaccagc ggcatgctct
     7741 ccttgctctc cacgtccggg ggcggggagg ccatcgaggt cgcgatggtc gggagcgaag
     7801 gcttcgtcgg cgtccccatc atcctgaagg tgggcgtcac tccttactgg agcatggtgc
     7861 aaatccagtc cgacgccatg cggatcagcg ccgccgccct cggcgaagag ttcgaccgct
     7921 gcgaacagct ccacgacctg ctcctgaaat acacacactc gctgctcgcc cacgtcacgc
     7981 agtcggcggt ctgcaaccgc ttccacacgg tgcaggagcg gctgtgccgc tggctgctcc
     8041 tcagccgcga ccgcatgaag tcggacacca tcaacctcac gcaggagttc atctcgcaaa
     8101 tgctcggcac cgcccgcacg ggcgtcacga tggccgccgt cccgcttcag gacgcggggc
     8161 tgattcgcta ccgccgcggc aagatcacga tcctcgaccg cgagggcctg gagtcggtcg
     8221 cctgcgagtg ctactcgata gtcaggaaag aggtcagcca caccctcgcc gcgtaaaaag
     8281 ccttcggctc cccctttatg tctgacgcaa gacagag
//
LOCUS       DBNGOH010001171         4553 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4579307, whole genome shotgun sequence.
ACCESSION   DBNGOH010001171 DBNGOH010000000
VERSION     DBNGOH010001171.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4553)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4553)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4553
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4579307"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..579
                     /locus_tag="AC159P_22680"
     CDS             <1..579
                     /locus_tag="AC159P_22680"
                     /inference="COORDINATES: protein motif:HMM:NF013740.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HIR3698307.1"
                     /translation="RFSLPLEGVKAGEIIERTREVISASVYGVLVIATIQGALGGLIF
                     FALGLPSPLLWGVVMMFFSMIPMAGAFIVWVPAAIYLLITGSYVKAVILIAFGALAIG
                     SVDNFLRPKLVGERTRLHELLIFFSVLGGLQFFGVLGLVLGPAVVAITLALLDIVRQA
                     GASAAETPLPEDTLIEEQAKLRDVHPTAASTP"
     gene            679..1263
                     /locus_tag="AC159P_22685"
     CDS             679..1263
                     /locus_tag="AC159P_22685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008239938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HIR3698308.1"
                     /translation="MTSEETVNPWRTISSKRVYENDWISVREDAVVRPDGLPGIYGTV
                     HFKHVAVGVLAVEGDEVYLIGQYRYPLDAYSWEIPEGGCAEGEDPLEAAGRELEEETG
                     LRAEHWVRLGEAHLSNSVSDEFALWFLATGLTKGEQRPEGSERIVVRKVKLARALAMA
                     LSGEITDALSLLAIMGYHFMRSAERTPPQGNAAG"
     gene            complement(1363..1599)
                     /locus_tag="AC159P_22690"
     CDS             complement(1363..1599)
                     /locus_tag="AC159P_22690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3698309.1"
                     /translation="MTETFPLAERLKAIDHIQARRYTKLMGTALDIATEGIIRHLRAC
                     DRMDVNPDVAAVREIIDDALNGRRVFAETFNNAA"
     gene            complement(2039..2347)
                     /locus_tag="AC159P_22695"
     CDS             complement(2039..2347)
                     /locus_tag="AC159P_22695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013582077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030527 - structural constituent of
                     chromatin [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HIR3698310.1"
                     /translation="MATKAGKKRMSQSEVVNHFAEKFGLKRAQVKELFEELASLASSE
                     VKESGEFVLPGFGKLALSERKAREGRNPQTGETIQIPAKTTLKFRLAKGMKDTVVPKK
                     "
     gene            complement(2611..2684)
                     /locus_tag="AC159P_22700"
     tRNA            complement(2611..2684)
                     /locus_tag="AC159P_22700"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2648..2650),aa:Pro,seq:tgg)
     gene            complement(2749..3378)
                     /gene="rdgB"
                     /locus_tag="AC159P_22705"
     CDS             complement(2749..3378)
                     /gene="rdgB"
                     /locus_tag="AC159P_22705"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035870 - dITP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0036222 - XTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HIR3698311.1"
                     /translation="MTARPELLIATTNPGKIAELTEFFAGLVPHLRSLSEFAATPEAE
                     ETGTTFKQNAVIKALHYSAHTGLWTLADDSGLEVEALGGAPGVYSARYGGSSASYPER
                     MARLLAEIAQTGDAARRARFVCVIALATPGDRDIHTFTGECRGRLARAPRGTGGFGYD
                     PVFIPDGHAETFGELPAEAKRNLSHRARAVAKVRDHIREKFSETGLTRS"
     gene            3649..>4553
                     /locus_tag="AC159P_22710"
     CDS             3649..>4553
                     /locus_tag="AC159P_22710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3698312.1"
                     /translation="MALGVWLRALQSYSEGSIASAGEAGEEFLLKRDYADDARIVHEV
                     LLRCSQLAVNLAQTESAEAGTGMHALCEIIRDSTHLAEAMLKSPGVGLQGWSSFNNVL
                     LRELNNSEAAGRVKSAVAAESAQAIPQELSAITEKITPEALGADFHAVFAALTRQLEY
                     LNFIETTLKGGRTLKPLLPVFTLLSRESFELLEHIEKRALKYPHAEKGILEALDGTAY
                     AVRMELRKAFDHELAGVRGERQPHYVYAKFENAHGLLRNCFQQSIVSLAQLFEPELEG
                     TELFEFFQTKLDESLSLRRELWKLLR"
BASE COUNT          864 a         1374 c         1462 g          853 t
ORIGIN      
        1 cgcttctcgc tcccgctcga aggcgtgaag gccggcgaga tcatcgagcg cacgcgcgag
       61 gtcatcagcg cgagcgtcta cggcgtgctc gtcatcgcga cgattcaggg cgcgcttggc
      121 gggctgatct tcttcgcgct cggactgcct tcgccgctgc tctggggcgt ggtgatgatg
      181 ttcttctcga tgatcccgat ggccggcgcc ttcatcgtct gggtgccggc ggccatctac
      241 ctcttgatca cggggagcta cgtcaaggcc gtcatcctca tcgcgttcgg cgcgctcgcc
      301 atcggctcgg tggacaactt cctgcgcccg aagctggtcg gcgagaggac gcggctgcac
      361 gagctgctga tcttcttctc cgtgcttggc gggttgcagt tcttcggcgt cctcggcctc
      421 gtgctcggcc ccgcggtcgt cgccatcacg ctcgcgctgc tcgacatcgt caggcaggcg
      481 ggcgccagcg ccgccgagac gccgctcccc gaagacacgc tcatcgaaga acaggcgaag
      541 ctgcgcgacg tgcatccgac cgccgcctcg acgccctgac gccgtcggcg cctgtccatt
      601 cacaagttct gacaccgaat ttcagaccgc agcccgtcgc gcggcgttgt tgcgcgcggc
      661 tgcacgcgga cggcaaatat gacctcggaa gaaaccgtca acccctggcg cacgatctcc
      721 tcgaagaggg tttacgagaa cgactggatc agcgtccgcg aggacgcggt cgtgcgcccg
      781 gacgggctgc cgggcatcta cgggacggtg catttcaagc acgtcgccgt cggcgtcctc
      841 gccgtcgagg gcgacgaagt ctacctgatc ggccagtaca ggtatccgct cgacgcctac
      901 tcttgggaga tacccgaagg cgggtgcgcg gaaggcgaag accctctgga ggccgccggg
      961 agggaactgg aggaagagac cgggctgcgc gcggagcact gggtcagact cggggaagca
     1021 cacctctcga attcggtcag cgacgagttc gcactctggt tcctcgcgac cggcctcacg
     1081 aagggggaac aacgccccga ggggtctgag agaatagtcg tccgcaaggt gaaactcgcg
     1141 cgcgccctcg ccatggcgct gagcggcgag atcaccgacg cgctgagcct cctcgccatc
     1201 atgggctatc acttcatgcg ctccgccgag aggactcctc ctcaaggcaa cgcggctggt
     1261 taaacgctgc ctcgacggag caaaaagaga ggccgcgcgc tttgccaaag cgcgcggcct
     1321 ctctagtgtt gtctcgcgcg agcagtggct cgcacgaaaa gctcaggcgg cgttgttgaa
     1381 ggtttcggcg aacacgcggc gcccgttgag ggcgtcgtcg atgatctcgc gaaccgccgc
     1441 cacgtcaggg ttcacgtcca tgcggtcgca ggcgcgcagg tgacggatga tgccttcggt
     1501 cgcgatgtcg agggcggtcc ccatcagttt cgtgtagcgg cgcgcctgaa tgtggtcaat
     1561 cgccttcagc cgttcggcca gcgggaatgt ttctgtcacg atcacttttc gtcatctcct
     1621 taatcttccg gccctctcga aaaagataag ttggaggccc cgaattacgt aaaatttaga
     1681 tgcaaggaac aaatcacggg atgcgattcg attgcgattc ccggaaattt acacaaacta
     1741 acccctccag cgcttcggca ccacaccctg cggggaaaag agggggttag gaaaagcgat
     1801 atactaaatg aatcttccgc aaaaatccag aaaaattttc ggcgggcctc ttcaccttcg
     1861 aggagccggg cgggcgggaa ggcgggaatt cgttgatccg tccaagtgaa gcaatataaa
     1921 ggcttagtcg tgtgctggtg aaagaggcac cgcccgcatt gtttgtcttc cgaagacaaa
     1981 tcaatacggg cggccttctt cgtatcgatg cgaaaatgac ggctcgtgaa gtgctgcgct
     2041 acttcttggg caccaccgta tccttcatgc ctttggccaa gcggaacttt agagtggttt
     2101 tggccggaat ctgaatcgtt tcgcccgtct gagggttgcg gccttcgcgg gccttccgct
     2161 cggacaaagc cagcttgccg aagccaggca gcacgaactc gccgctctct ttgacttcgc
     2221 tgctggccag actggccaac tcttcgaaaa gctctttcac ctgagcacgt ttcagaccga
     2281 atttctcggc gaaatggttt acgacctccg actgggacat gcgctttttg cctgctttcg
     2341 tcgccatagc ctcgtgtgac tacctcctgc aaaccgggaa aaaattgaaa agctgtacgg
     2401 gacatatatc acacagaacg gccccgcgtc tcgccaaaaa gccacttttt taaggaattt
     2461 tcaaaaaata actttcggtc gcgtcgcgcg aagccttccg ttaataaaga tacacaaccc
     2521 cgactccaca tggatcgaac gccatgccgg atagtccgtg aggcggcggc gggacggcat
     2581 cggcggcggg atgaatatca gggaaatgaa tcggggcggc aggattcgaa cctgcgacct
     2641 ctcggtccca aaccgagcgc actaccaggc tgtgctacgc cccgaaaaac gagtcgatat
     2701 aaaaagctga aaaggcaggc ttttaacgaa cggattctag ccagtgcatc atgaccgggt
     2761 caagccggtc tccgaaaatt tctcccggat gtggtcgcgg actttcgcta cggcgcgcgc
     2821 gcggtggctg agatttcgtt tggcttccgc gggcagctcg ccgaatgttt cggcgtgacc
     2881 gtcggggatg aagacggggt cgtagccgaa accgcctgtg ccgcggggcg cgcgggccaa
     2941 gcgcccgcgg cactcgcccg tgaaggtgtg gatgtcgcgg tcgccgggcg tggcgagcgc
     3001 gatgacgcag acgaagcgtg cgcggcgcgc ggcgtcgccg gtctgtgcga tctcggcgag
     3061 gagccgtgcc atgcgttctg ggtaagaggc gctgctcccg ccgtagcgcg cggagtagac
     3121 gccgggcgcg ccgccgaggg cctcgacctc aagccccgag tcgtcggcca gcgtccacag
     3181 gccggtgtgc gcggagtaat gcagcgcctt gatgacggcg ttctgcttga aggtcgtccc
     3241 ggtctcctcg gcttcgggcg tggccgcgaa ctccgagagg gaacgcaggt ggggcacgag
     3301 gcccgcgaaa aattccgtca gctccgcgat ctttccgggg ttggtggtgg cgatcagcaa
     3361 ttcggggcga gcggtcatgg ggcgtttacc tgtgacgaag cgtgaatggt tttggatgaa
     3421 acgagccgcg ggcctgtggt cactaaaatg ttttgcccgc cggagctttc cgcgtttaaa
     3481 atccgcggag agattttcac gcgacaccgc gtcttacctg cgggccatcg gcgccacgcg
     3541 cgaggacggc tgccgccggg aggaagtctc tctaccgggg ggaagagtca gacacatcag
     3601 atgacgatca atcacagtcg cttcgacggc gaaggaattt ccggcccgtt ggcactcggg
     3661 gtttggctgc gcgcgcttca gagctattcg gagggcagta tagcttccgc gggcgaggcg
     3721 ggcgaagaat ttctcctcaa gcgggattac gcggacgacg cccgcatcgt ccacgaagtc
     3781 ctgctgcgtt gttctcagct cgccgtcaac ctcgcgcaga cggagtcggc ggaggcgggc
     3841 acggggatgc acgcgctgtg cgaaatcatc agggactcga cacacctcgc cgaggcgatg
     3901 ctgaagagtc cgggcgtcgg acttcagggg tggtcgagct tcaacaacgt cctgctgcgc
     3961 gagctgaaca actcggaggc agcggggcgc gtgaagtccg ccgtcgcggc ggagagcgcg
     4021 caggcgatcc cgcaggagct ttcagccatc accgagaaga tcacgcccga ggcgctcggc
     4081 gcggacttcc acgccgtttt cgcggcgctg acacggcagc tcgaatacct gaatttcatc
     4141 gaaaccactt tgaagggcgg gcggacgctg aagccgctgc tgccggtctt cacgctcttg
     4201 agccgcgagt cgttcgagct gctcgaacac atcgagaagc gcgccctcaa gtatccccac
     4261 gcggagaagg ggattctgga ggcgctcgac ggcacggcct acgccgtcag gatggagctg
     4321 cggaaggctt tcgaccacga gcttgcgggc gtgaggggcg agcgccagcc gcactacgtc
     4381 tacgcgaagt tcgagaacgc gcatggcctg ctgcggaact gcttccagca gtcgatcgtc
     4441 agcctcgcgc agttgttcga gccggaactg gaggggacag agctgttcga attcttccag
     4501 acgaagctcg acgaatcgct ctcactccgc cgcgagctgt ggaagctgct gcg
//
LOCUS       DBNGOH010001172         3229 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_180175, whole genome shotgun sequence.
ACCESSION   DBNGOH010001172 DBNGOH010000000
VERSION     DBNGOH010001172.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3229)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3229)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3229
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_180175"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..904
                     /locus_tag="AC159P_22715"
     CDS             <1..904
                     /locus_tag="AC159P_22715"
                     /inference="COORDINATES: protein motif:HMM:NF012379.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HIR3698313.1"
                     /translation="GMELQDLRPALEGTTFAPFASALEKRGEVKALVVKGGAHLSRKQ
                     LDELQEFARRYGAGALAWVKTGDELHSSLLKALGEEAVARVAAQAGAEKGDAVLVVAG
                     KASVVAASLGALRNEVARREKLIPAGAFAPLWVTEFPMFEFHEEDGRYYAMHHPFTAP
                     LDEDLPLLERAVEGGETHLLGQLRAKAYDPVINGVELASGSIRIHRRDVQHRIFKALG
                     MSEEEARSRFGFFLEALEYGTPPHGGIAPGIDRLMMLLAGTDNIRDVIAFPKTASAAD
                     LMLDAPSEIDRKQLKELGIKIVNE"
     gene            958..1515
                     /locus_tag="AC159P_22720"
     CDS             958..1515
                     /locus_tag="AC159P_22720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3698314.1"
                     /translation="MKLLAVLLLVGLCGAAVAAQDKWAVADSAPVRLPPAAFSRLPKN
                     IARYLQARGCTIPQTSLSSEPHNVISGAFAVRGQTDWAVLCSRTGESSILVFRRGSTN
                     SVAEVGKAPDRNYLQNVTGGGKAAFSRVIEPVGRDYILRHHKEYGGRRPPRVTHQGIN
                     DAYAEKASVVRCYRRGRWLELQGAD"
     gene            1617..2510
                     /locus_tag="AC159P_22725"
     CDS             1617..2510
                     /locus_tag="AC159P_22725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011422180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-70 factor"
                     /protein_id="HIR3698315.1"
                     /translation="MDEAGDRSAVFERLRGRLFAVAYRMTGTRADAEDIVQEAYLRWH
                     QADTSEVRSAEGWLVSVTTRLSIDRLRKASAEREKYTGQWLPEPLFGAPQRSPEEQLE
                     LASDLSMAFMVLLERLAPVERAALLLHDVFDSSYPEIARILGKGEAACRQLVHRARQR
                     VRRDHTRFRVGEEDRRRLIEKFMEAAYAGDEATLHALFAAEATLTSDGGGVVPAARKV
                     IRGRGRIARLFLVVARKLRGRLTHAVQTINGEPGLVIYLDGAPFSATSFETDGRSILA
                     LYHVLNPQKLTGIRPPQDTPA"
     gene            2677..3147
                     /locus_tag="AC159P_22730"
     CDS             2677..3147
                     /locus_tag="AC159P_22730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HIR3698316.1"
                     /translation="MKQRVNYAEVGRGAFKAMYGLEAYLAKCGLEPSLLHLLKFRVSQ
                     INGCAYCLDMHSKDLRAAGETEQRLYLLDAWREAPFYTERERAALAWAEAVTLVTEGH
                     VPDEVYERARAAFSEEELVNLTLAVVAINGWNRLSIAFRVEPGSYQPATTHATA"
BASE COUNT          548 a         1101 c         1091 g          489 t
ORIGIN      
        1 cgggatggag ctgcaagacc tgcgccccgc gttggagggg acgacgttcg cgccgttcgc
       61 ctcggcgctg gagaagcggg gcgaggtcaa ggcgctcgtc gtcaagggcg gcgcgcacct
      121 ttcgcgcaaa cagctcgacg agttgcagga gttcgcccgc cgctacggcg cgggcgcgct
      181 cgcgtgggtc aagacgggtg acgagctgca ctcctcgctg ctcaaggcgc tgggcgagga
      241 ggcggtcgcg cgcgtcgccg cgcaggccgg cgccgagaag ggtgacgcgg tgctcgtcgt
      301 cgcgggcaag gcttcggtcg tcgcggcctc tctgggcgcg ctcaggaacg aggtcgcgcg
      361 gcgcgagaag ctgatccccg cgggcgcctt cgcgccgctc tgggtgacgg agttcccgat
      421 gttcgaattc cacgaggagg acgggcgcta ctacgccatg caccacccgt tcaccgcgcc
      481 gctcgacgaa gacctgccgc tcctggaacg cgccgtcgag ggcggcgaga cgcacctgct
      541 cggccaactc cgcgccaaag cctacgaccc cgtgattaac ggggtggagc tggcgagcgg
      601 ctccatacgt attcaccggc gcgacgtgca gcaccgcatc ttcaaggcgc tcggtatgtc
      661 ggaggaggag gcgcgctcgc gcttcggctt cttcctcgaa gcgctggagt acggcacgcc
      721 gccgcacggc ggcatcgcgc cgggcatcga ccgcctcatg atgctgctgg cgggcaccga
      781 caacatccgc gatgtgatcg ccttccccaa gacggcctcc gccgccgacc tgatgctcga
      841 cgcgccgagc gaaatcgacc ggaagcagtt gaaggaactc ggcatcaaga tcgtcaacga
      901 atgagccgca ccgtcaacgg ttgagccaac ccatctcttt gcgggaggca tgtcggcatg
      961 aaactcctcg cggttctact tctcgtcggc ctgtgtgggg cggcggtcgc ggcgcaggat
     1021 aagtgggcgg tcgcggactc cgcgcccgtg cgcctcccgc ccgcggcctt ctcacgtctt
     1081 ccgaaaaaca tcgcgcgtta tcttcaggcc cgcggctgca cgattccgca aacctccctc
     1141 agcagcgagc cgcacaacgt catcagcggc gcgttcgccg tacgggggca aaccgattgg
     1201 gccgtgctct gttcgcgcac aggggaatcg tccatcctcg tcttccggcg cggctcgacg
     1261 aactccgtcg cggaggtcgg caaagctccc gaccgcaatt acctgcaaaa cgtgaccggg
     1321 ggcgggaagg ccgccttctc ccgtgtgatc gaacccgtcg gcagagatta catcctgcgc
     1381 caccacaaag agtatggggg ccgcaggccg ccccgcgtca ctcatcaggg catcaacgac
     1441 gcctacgcgg agaaggcttc ggtcgtgcgc tgctaccgcc gcggcagatg gctggagctg
     1501 caaggcgcgg attagacggt ctccccgaac cgcccggtgc cgaacagcac gacacgttgt
     1561 cacaaacgcg ggcacttgtc cgtctaggca tgtgaaggga ttgttccgtg gtggtaatgg
     1621 atgaagcagg cgaccgcagc gcggtgttcg agcggctccg ggggcggctc ttcgccgtcg
     1681 cctaccggat gacggggacg agggcggacg ccgaggacat cgtgcaggag gcatatctgc
     1741 gctggcatca ggcggacacg tccgaggtgc gctccgctga aggctggctc gtcagcgtga
     1801 cgacgaggct ttccatcgac cgcctgcgca aggcgtcagc cgagcgcgag aagtacacgg
     1861 ggcagtggct ccccgagccg ctcttcggcg ctccccaacg ctcgcctgaa gagcagctcg
     1921 aactcgcctc cgacctctcg atggccttca tggtgctcct cgaacgcctc gcgcccgtcg
     1981 agcgcgcggc cctcctgctc cacgacgttt tcgactcctc ctaccccgag atcgcacgca
     2041 tcctcggcaa gggcgaggcc gcctgccgcc agttggtgca ccgcgcgcgg cagcgcgtgc
     2101 ggcgcgacca cacgcggttc cgcgtcggcg aggaagaccg ccgcaggctg atcgagaagt
     2161 tcatggaggc cgcctacgcg ggcgacgagg cgacgctcca cgcactcttc gccgcggaag
     2221 cgacgctgac gagcgacggc ggcggcgtgg tgcccgccgc gcgcaaggtc atccgcgggc
     2281 gcgggcgcat cgcgcgcctc ttcctcgtcg tcgcgcgcaa gctccgcggg cggctgacac
     2341 acgccgtcca gaccatcaac ggcgaacccg gcctcgtcat ctacctcgac ggggcgccgt
     2401 tctcggccac ctcgttcgag accgacgggc gctccatcct cgccctctat cacgtcctca
     2461 acccgcaaaa actgacaggc atccggccac cccaagacac gcccgcttga agcggcgcgc
     2521 gtttcgaaat aggctgtcac aaaaacgcac ccgcgtccgt cttcgttatg aaggcgcgat
     2581 tgccgtggcc gcaagacccg agcgcacgcg cgcgagacag gggccacggg cgcgggcaac
     2641 ggcttcgaca tatatttctt cgaaggagag gaagtcatga aacagagagt gaactacgcg
     2701 gaagtcggac ggggcgcgtt caaggcgatg tacggtttgg aggcatacct ggccaagtgc
     2761 gggctggagc cgtcgctgct ccatctgctg aagttccgcg tgtcgcagat caacggctgc
     2821 gcctactgcc tcgacatgca ctcgaaggac ttgcgcgccg caggcgagac ggagcagcgg
     2881 ctctacctgc tcgacgcctg gcgcgaggcg cctttctaca ccgagcgcga gcgggcggcg
     2941 ctcgcgtggg ccgaggccgt gacgctcgtc acggaggggc atgtccccga cgaagtctac
     3001 gagcgggcgc gggccgcgtt cagcgaagag gagctggtca acctgacgct ggccgtcgtg
     3061 gccatcaacg gctggaaccg cctgagcatc gccttccgcg tggagccggg cagttaccag
     3121 ccggccacga cgcacgcgac cgcatgagcg ttggcagttc ggagcgccaa cgcgcgtgaa
     3181 gactgtaggg gcagggcaag tccgcggccc gccgctacgg atgcattca
//
LOCUS       DBNGOH010001173         1671 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1831060, whole genome shotgun sequence.
ACCESSION   DBNGOH010001173 DBNGOH010000000
VERSION     DBNGOH010001173.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1671)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1671)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1671
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1831060"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            181..864
                     /locus_tag="AC159P_22735"
     CDS             181..864
                     /locus_tag="AC159P_22735"
                     /inference="COORDINATES: protein motif:HMM:NF022142.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2490 domain-containing protein"
                     /protein_id="HIR3698317.1"
                     /translation="MSIHTPLKSVAFTLTALTVMLFSAGHASGQQAPQDDTQFWNDVQ
                     IATALDKRIDLVFNGTLRIGRDVTHPVDERAGVGLSIKAGKYLTLAPNYTYIATQPVA
                     GRKGYESRLTFAVTARKQFEKLVVTDRNQFERRLFNSRADATRYRNRLQFEYPVKLWG
                     HDLKLFASDEIFYDWSVDEWVRNRFAAGGSKAFNKHFTLDLYYMRQNDGRSRPGDLHV
                     VGATYRVRI"
     gene            1171..1536
                     /locus_tag="AC159P_22740"
     CDS             1171..1536
                     /locus_tag="AC159P_22740"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3698318.1"
                     /translation="MDNLDDVRGVIGGWLAREGYRVVEAKDGPGAVEEARRLVPDLIL
                     MDLKMPGMDGIAAAQAIRGDVALRGVPIVAVSADNTEYYKGKAREAGFSDYLVKPFAA
                     EELRGVLDRLLPRNGAVIH"
BASE COUNT          341 a          498 c          558 g          274 t
ORIGIN      
        1 gcagtacccg gcagtggaag acctgtggaa ggtcgattac ctgggcggct ggtcgagggt
       61 ctcgagggag atttacgggc cgcagggggt gtacacgaag atgttcgacg agctgcgcgg
      121 gcagaagccg tgaagtcaag cgtcggcgga gtccggcggc aacagggaaa ggagtctatc
      181 atgtcaattc acacgcctct caaatccgtg gccttcacgc tgacggccct gaccgtgatg
      241 ctgttctccg cgggccacgc ttcgggacaa caggccccgc aggacgacac acaattctgg
      301 aacgacgtgc agatcgcgac cgcgctcgac aagcggatcg acctggtctt taacggcacg
      361 ctccgcatcg gtcgcgacgt gacccacccg gtggacgagc gcgcaggcgt cggcctctcg
      421 atcaaggcgg gtaagtacct caccctcgcc ccgaactata catacatcgc gactcagccg
      481 gtcgccgggc ggaagggtta cgagagcagg ctgaccttcg ccgtgaccgc gcgtaagcag
      541 ttcgagaagc tggtcgtcac cgaccggaac cagttcgagc gccggctgtt caactccagg
      601 gccgacgcca ccagataccg caaccgcctg cagttcgaat accccgtcaa gctgtggggc
      661 cacgacttga agctgttcgc ctcggacgag attttctatg actggagcgt ggacgaatgg
      721 gtgcgcaacc gcttcgccgc gggcggcagc aaggcgttca acaagcactt cacgctcgac
      781 ctttattaca tgaggcagaa cgacgggcgc agccgccccg gcgacctcca cgtcgtcggc
      841 gcgacctaca gggtcaggat ttaatctctt cttccggtgt gcaacggtgt ggttacaggc
      901 ggcgtcgggc catcgtaatt ggcttgcaac accgccgcaa ccaacggcct gccggccccg
      961 tctatcatcg ccggacaatc aacgcgaaac acacgcgtgt gtttcgaaaa atcggagcgc
     1021 cgggctggct aaagtccggc gcttctttac acagaggacg cgaagcgcat tgcgacacga
     1081 aggcaaagcc acgagaagag ttgagcgagg aggtgaaaga tgtcgagtca tcgagagagt
     1141 ttcagtaacc accctcagac tatcttggta gtggacaacc tggacgatgt ccgcggcgtc
     1201 atcggcggct ggctcgcacg tgagggctac cgcgtggtgg aggcgaagga cgggccgggc
     1261 gcggtcgaag aggcgcggcg gctcgtcccc gacctgatcc tgatggacct caagatgccc
     1321 ggcatggacg ggatcgcggc ggcgcaggcc attcgcggag acgtggcgct gcggggcgtg
     1381 ccgattgtgg ccgtctccgc cgacaacacc gaatactaca agggcaaggc gcgcgaggcg
     1441 ggtttcagcg actacctggt gaagcctttc gcggcggagg aactgcgagg ggtgttagac
     1501 cggctgctgc cgcgaaacgg cgcggtgatt cattgacgat caagagtccg cagccggggc
     1561 gagggcgcgg cggggtgtca ataccccccg cgcccgtttt tatgtgtaca ggaatacgcg
     1621 tgccgtcgca agacgaggcc acgcggaggg ggtgaaaacc gagccccggt t
//
LOCUS       DBNGOH010001174         2922 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4944995, whole genome shotgun sequence.
ACCESSION   DBNGOH010001174 DBNGOH010000000
VERSION     DBNGOH010001174.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2922)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2922)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2922
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4944995"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..783)
                     /locus_tag="AC159P_22745"
     CDS             complement(<1..783)
                     /locus_tag="AC159P_22745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3698319.1"
                     /translation="MHRHRQVQRLKLTLVLLTLAALAATLWNGGGSSARDLSATRGDE
                     SARVDRNQTAAPAQPQQTPATAPATTPPAPAQSPPRVEGCISCHGGTEPMHRTRDGKL
                     KADGTDGQNLSCTYCHGGNPFAALGESQKTLRRGEKEFDKIMDSAHVRPRFPKEWEGK
                     DGKYSSANPERTNALLARESREFVRFVNPGDFRVAELTCSSSGCHTAEVGATRNSMMR
                     HGAMLWGAALYNNDGFPLKDTRFGESYSEERGAPERLIQQGEI"
     gene            complement(842..>2922)
                     /locus_tag="AC159P_22750"
     CDS             complement(842..>2922)
                     /locus_tag="AC159P_22750"
                     /inference="COORDINATES: protein motif:HMM:NF025006.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HIR3698320.1"
                     /translation="RGRLTGRVLDSAGAQPAPAEGVYVVAVNQVSGHSWREKSKSGGQ
                     YSFSLPAGAYRVSVESPARANFARDGKFAELALPRGEYIENVYVEPGRDTTLDIPLAK
                     RDETAAATPAPATKGGGEGEEPLGYTGDPSVESEAATRPDRVNVRDRWRAGFPEYDRY
                     GSVAGGRDIPFRKGRWYNPYDQSVLKGDFPILGQDIFLIITGTSTFTAQQQRTPTPSN
                     LSSARPGSNEFFGKPEILGLNNVLQFSVEMFSGQTTFRPRSWAFKLSPTLSIPNYVRA
                     REQSILNIDPRKGTTRTDTHFSFEDAFVEVKLDEVNKNFDFISVRAGIQPFVSDFRGF
                     IYTDNNLGARIFGGFDNNRYNYNFAYFAQLEKDTNSGLNRFDRRRQNVYIANLYRQDF
                     LMKGYTGQVSFHFNDDRAGIELDRNGFQVRPAVVGDVRPHAVRVGYLGLSGDGHLGRI
                     NLTNSYYFALGTDTRNPIAGRKTRIKSHMAVAEASIDYDWQRYRISAFFASGDPDPTD
                     DRANGFDAILDDPNIIGGQFSFWNRVGIPLAGTAVGLVQPLSVLPSLRSSKTQGQANF
                     VNPGIFIFNVGYDAEVTQRIKAIFNYNYLRFHRTESLEYLLFQNRIRHEIGHDLSVGV
                     AYRPLLINNIQFTFGASMLKPGRGFRDIYTDRTRNCPENVQDFCTPDGVNINPAKPLY
                     AIFGQAKFTF"
BASE COUNT          417 a          844 c         1115 g          546 t
ORIGIN      
        1 gatctcgccc tgctggatga ggcgttcggg cgcgccgcgc tcttccgagt agctctcgcc
       61 gaagcgcgtg tctttcaagg ggaagccgtc gttgttgtag agcgccgcgc cccagagcat
      121 cgcgccgtgg cgcatcatgc tgttgcgcgt ggccccgacc tcggccgtgt ggcagcccga
      181 ggaactgcag gtcagctcgg cgacgcggaa gtcgccgggg ttgacgaagc ggacgaactc
      241 gcggctctcg cgcgcgagta gggcgttggt gcgctcgggg ttggcgctcg aatacttgcc
      301 gtccttgccc tcccactctt tggggaagcg aggccggacg tgcgcggagt ccataatctt
      361 atcgaactct ttttcgccgc gccgcaacgt cttctgcgac tcgccgagcg cggcgaacgg
      421 gttgccgccg tggcagtagg tgcacgagag gttctgcccg tcggtgccgt cggccttgag
      481 cttgccgtcg cgcgtgcggt gcatcggctc ggtgccgccg tggcaactga tgcagccttc
      541 gacacgcggc gggctttgcg cgggggcggg cggcgtggtc gccggtgcgg tcgcgggcgt
      601 ctgctgcggc tgcgcgggcg cggcggtctg gttcctgtca acgcgcgccg actcgtctcc
      661 cctcgtcgcg cttaggtctc gcgcggaaga gccaccgccg ttccacagcg tcgcggcgag
      721 cgcggccagc gtcaggagga cgagggtcag cttgagacgt tgaacttggc ggtgccggtg
      781 cattgaacct ccgtgggtac gcgtgcgtgt gtcgggtttc gggcgaataa agctcgggcg
      841 cttagaaagt gaacttggcc tgaccgaaga tggcgtagag cggcttggcc gggttgatgt
      901 tgacgccgtc gggcgtgcag aagtcctgga cgttctcggg gcagttgcgc gtgcggtcgg
      961 tgtagatatc gcggaagccg cggcccggct tgagcatcga ggcgccgaag gtgaactgga
     1021 tgttgttgat caggagcggg cggtaggcga cgccgacgct gaggtcgtgg ccgatctcgt
     1081 ggcggatgcg gttctggaac agcaggtatt cgagcgactc ggtgcggtgg aagcgcaggt
     1141 agttgtagtt gaagatggcc ttgatgcgct gcgtgacttc ggcgtcgtag ccgacgttga
     1201 agatgaagat gccggggttg acgaagttgg cctgcccctg cgtcttgctg ctcctgagcg
     1261 aggggagcac gctcaagggc tggacgaggc cgacggcggt ccccgcgagc gggatgccga
     1321 cgcggttcca gaaggagaac tggccgccga tgatgttggg gtcgtcgagg atggcgtcga
     1381 agccgttggc gcggtcgtcg gtggggtcgg ggtcgcccga ggcgaagaag gccgagatgc
     1441 ggtagcgctg ccagtcgtaa tcaatcgagg cttcggcgac ggccatgtgc gacttgatgc
     1501 gcgtcttgcg tccggcgatc gggttgcgcg tgtctgtgcc gagcgcgaag tagtaggagt
     1561 tggtcaggtt gatgcggccc aagtggccgt cgcccgaaag gccgaggtag ccgacgcgga
     1621 cggcgtgggg ccgcacgtcg ccgacgaccg cggggcgcac ctgaaagccg ttgcggtcta
     1681 actcgatgcc ggcgcggtcg tcgttgaagt ggaagctcac ctggccggtg tagcccttca
     1741 tcagaaagtc ctgccggtag aggttggcga tgtagacgtt ctggcggcgg cggtcgaagc
     1801 ggttcaggcc gctgttggtg tccttttcga gctgcgcgaa gtaggcgaag ttgtagttgt
     1861 agcggttgtt gtcgaagccg ccgaagatgc gcgcgccgag gttgttgtcg gtgtaaatga
     1921 acccgcgaaa gtccgacacg aagggctgaa tccctgcgcg cacggagatg aagtcgaagt
     1981 tcttgttgac ctcgtcgagc ttgacttcga cgaaggcgtc ctcgaaggag aagtgcgtgt
     2041 cggtgcgcgt cgtgcctttg cgcgggtcga tgttgaggat cgactgctcg cgcgcgcgga
     2101 cgtagttcgg gatgctcagg gtcggcgaca gcttgaaggc ccacgagcgc gggcggaaag
     2161 tggtctgccc gctgaacatc tcgacggaga actggagcac gttgttgaga ccgagaattt
     2221 cgggcttgcc gaagaactcg ttgctgccgg ggcgcgcgct cgacaggttc gagggcgtgg
     2281 gcgtgcgctg ttgctgcgcg gtgaaggtgc tcgtgccggt gatgatcagg aagatgtcct
     2341 gcccgaggat ggggaagtcg cccttgagga cgctctggtc gtaggggttg taccagcggc
     2401 ccttgcggaa ggggatgtcg cggccccccg cgacgctgcc gtagcggtcg tattcgggga
     2461 agcccgcgcg ccagcggtcg cggacgttga cgcggtcggg gcgcgtcgcc gcctcggact
     2521 cgacgctcgg gtcgcccgtg tagccgagcg gctcttcgcc ctcgccgccc cccttagtag
     2581 cgggcgcggg cgtcgccgcc gccgtctcgt cccttttcgc cagagggatg tcgagcgtcg
     2641 tgtcgcggcc cggctcgacg tagacgttct cgatgtactc gccgcgcggc agcgccagct
     2701 cggcgaactt gccgtcgcgc gcgaagttgg cgcgcgcggg cgactcgacg ctgacgcggt
     2761 aggcgcccgc cggcagactg aaagaatact gcccgcccga cttgctcttc tcgcgccacg
     2821 agtggccgct gacctggttg acggcgacga cgtagacgcc ttcggcgggc gcgggctgcg
     2881 cgcccgccga gtcgagcacg cggcccgtca gccgaccgcg ct
//
LOCUS       DBNGOH010001175         3244 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1646569, whole genome shotgun sequence.
ACCESSION   DBNGOH010001175 DBNGOH010000000
VERSION     DBNGOH010001175.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3244)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3244)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3244
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1646569"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..485
                     /locus_tag="AC159P_22755"
     CDS             <1..485
                     /locus_tag="AC159P_22755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3698321.1"
                     /translation="TLRSLVMSRLPVMDIYLVPINGDDSDEGRSFEPVDGRENPEQGL
                     LRREAESGRDGQVERFMEGLHAAVRGKTKQYDRMLGVPWHCYLNPRHHTQLEVAAMLG
                     VSDSLVSEYRRRIEHELRALPLAGVAEARAFEVALRERVPRLPGCSPPRSRNSLQTVA
                     "
     gene            complement(1164..1340)
                     /locus_tag="AC159P_22760"
     CDS             complement(1164..1340)
                     /locus_tag="AC159P_22760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3698322.1"
                     /translation="MTSVVVGYGCAALCVALLAFSIWASATGAGDGVGLVAVASAFVL
                     LMVCGATLREESER"
     gene            complement(1398..1802)
                     /locus_tag="AC159P_22765"
     CDS             complement(1398..1802)
                     /locus_tag="AC159P_22765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3698323.1"
                     /translation="MKHLFKRTVIFLCVCALTGVAALATTLTKEVAFSRALTVNGAPV
                     KAGTYKVTFDDQTGQLTVLDGKKVVAQAPARLDNLKGGSGYTTRTVGGSTVLIGVYMG
                     GNNQATIINDADLPTNSSNQATILKSIGVTAP"
     gene            2169..2735
                     /locus_tag="AC159P_22770"
     CDS             2169..2735
                     /locus_tag="AC159P_22770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3698324.1"
                     /translation="MKKSNNRIGGAVITLLMVAGAFLATSATAQAQYRQYPDGRVRQE
                     RRDRDDRYGRDRRAARRDRDGDGDYDRRDRAIRRRQRQQRNGGYGTYGRNGGYGNDGG
                     YNQAALNQGYQNGLNTGARDAQRGQSYNPQRSHFYKEASSQAFREGFVRGYDEGYRQY
                     AGNDNGDYRGRNTGGGIGDILGGILGRP"
     gene            complement(2933..>3244)
                     /locus_tag="AC159P_22775"
     CDS             complement(2933..>3244)
                     /locus_tag="AC159P_22775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014784714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HIR3698325.1"
                     /translation="RFRWVLPGMVVGVALWLAGSLGFRLYLRYFNTYNAVYGSLGAVI
                     ILMLWFYLTGAAILIGGEVNSEIEKAAAAGGAEGAKLPGEKSPGERADGSAPVEGAPA
                     A"
BASE COUNT          602 a         1055 c         1045 g          542 t
ORIGIN      
        1 gcacgctgcg cagcctggtg atgtcgcggc ttccggtgat ggacatctac ctcgtcccga
       61 tcaacggcga cgacagcgac gaggggcgct ccttcgagcc ggtggacggg cgcgagaacc
      121 cggagcaagg gctgctcaga cgggaggcgg agagtgggcg tgacggtcag gtggaacggt
      181 tcatggaagg tctgcacgcc gccgtgcgcg gcaagaccaa gcagtacgac cgcatgctcg
      241 gcgtgccgtg gcactgctac ctcaacccga ggcatcacac gcagctcgaa gtcgccgcca
      301 tgctcggcgt ttccgattcg ctcgtttcgg aataccggcg ccgcatcgag cacgagttac
      361 gcgcactccc tttagcgggt gtcgccgagg cgcgcgcttt cgaggtggcg ctgagggaac
      421 gtgtcccgcg ccttccgggc tgctcgcctc ccaggagtcg caactcttta cagacggtcg
      481 cttgaacgtg tcaacagacg aaccggggag ggcaatgaaa ccgctcacgc ttgttcagta
      541 cgaggccgcg cccgcattgc gtcaacccct ctcgctcgcg cccgtgctgg ccgccctgtg
      601 gctcatcacc tgcgcgcccg tcgttgctca cggacaagat tcgacctccc cacggcgccc
      661 gccggcacgg agcagaccgc gcccgtggcg accgaccgct tcgtggttcg gcgcatcaag
      721 cagaggactg acaaccgctt tgtcaggatc gtcgcgcgga tgatctttaa cccaaccagg
      781 agtagtgcga acattctcgg cttcgagaaa ccgtggcgcc gctacaggta gcagcgctca
      841 ccggcggcgt gcctgcggcg gttgtgatgc gtggaagaag cgcctcctgc tccggactcg
      901 gtattgaaat ggagatttct tgagtgacag atgagaccga caaagtaacc gaaggtctgg
      961 tcgcgcccga agactcgcac gtttccgaaa tccgctaccg gcggccctga cggctgacgc
     1021 gggcgaagaa gaccgcaccc tcctcccact cgcggagagt gcgggcagag taggttggtt
     1081 agcaacgagg aggaggcccg gggtatttct cccggaccga gccgcccctt ctccacattg
     1141 tttcgggctg atgacgcgcc gccctagcgc tcggactcct cgcgcagcgt ggcgccgcag
     1201 accatcagca ggacgaaagc cgaagccacc gccacgagcc caaccccgtc gcccgcgccc
     1261 gtggccgacg cccagatcga gaaggccagc agtgcgacgc acaacgcggc gcagccgtaa
     1321 ccgacgacaa cactcgtcat cccacttaac tcccctccgc ccggcaccgc ccgggcacgc
     1381 attcggaaga agacccgtta cggtgccgtg acgccgatac tcttgaggat cgtggcttga
     1441 ttgctgctat tcgtggggag gtcggcgtca ttgatgatcg tggcctgatt gttaccgccc
     1501 atgtagacgc ctatcaggac ggtgctgccg cccacggtgc gcgtcgtata cccggacccg
     1561 cccttgagat tgtcaagtcg tgccggcgct tgggccacaa ccttcttgcc gtcgaggacc
     1621 gtcagttggc cggtttggtc gtcgaacgtg accttgtagg tgcccgcttt aacgggcgcg
     1681 ccgttgaccg tcagggcgcg gctgaaagcc acttccttcg tgagtgtcgt cgccagcgcc
     1741 gcgacgcccg tcagggcgca cacgcagagg aatataaccg tccgcttaaa aaggtgtttc
     1801 attcttatct ccccagtgct tgagccatga gcttcaggtg atctggagga ttccagagga
     1861 gctgagaaag gagttaagcc aggactcgat ctgccggtat catatcacga acgatatatt
     1921 tcgcatacga tatattacgt atacgataac taatgggtgt caggaggcgc cgccgcgcgg
     1981 tcgtaggccg tcttgaacgg agtatctgcc gggggcggaa acttcactca tctgcgcggc
     2041 gtttgcacga gatggtgatg tgggctgagc cggtcggctc atccatccgg actttatcaa
     2101 gcattccgtc ttcgccgagc gtggcacgat ggttgccctg ttaatggcca cggaggatga
     2161 gcacaaacat gaagaaatcc aacaatcgta tcggcggcgc agtcataacg ctcctgatgg
     2221 tggcgggcgc ctttctggcc acgagcgcca cggcgcaggc gcagtaccgg cagtaccctg
     2281 acgggcgcgt ccgccaggag cgccgcgacc gtgatgaccg ttacggccgc gaccgccgcg
     2341 ccgcccgccg cgaccgtgac ggtgacggcg actacgaccg gcgcgaccgg gcaatccgcc
     2401 gccggcagcg gcagcagcgc aacggcggtt acggcaccta cggacgtaac ggcggttacg
     2461 gcaacgacgg cggctacaac caggcggccc tgaaccaggg ctaccagaac ggcctgaaca
     2521 ccggcgcgag agacgcgcag cgcgggcaga gctacaaccc gcagcggtcg cacttctaca
     2581 aggaagcctc gtcgcaggcg ttccgcgaag gcttcgtgcg cggctacgac gaagggtatc
     2641 gccagtacgc cggcaacgac aacggcgact accgcgggcg caacacgggc ggcggcatcg
     2701 gggacatcct cggcggcatc ctcgggcgac cgtagcgcgg cccgagacgc gattcgggtt
     2761 cgaagtgggt gagccgtctt cacccagtgc tgacgacaga gtaatcttag acggtggccg
     2821 accggcggca ctcttgatgc gggagcgtcg cggtgcaatc ggtcggccct tttgttaatg
     2881 gtcaccggcg ggtcaccaca gtggattatt caggcgacgc aagtgtttcc gcctacgcag
     2941 cgggcgcgcc ctcgaccgga gcgctcccgt ccgccctctc gccgggactc ttctcgcccg
     3001 gcagcttggc cccttcggcc ccccccgccg ccgccgcctt ctcgatttcc gagttgacct
     3061 cgccgccgat gaggatggcc gcgcccgtca ggtagaacca gagcatgagg atgatgaccg
     3121 cgccgagcga gccgtagacg gcgttgtagg tgttgaagta gcgcaggtag aggcggaagc
     3181 cgagcgagcc ggccagccac agcgcgacgc cgacgaccat cccgggcagc acccagcgga
     3241 acct
//
LOCUS       DBNGOH010001176         3004 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3845564, whole genome shotgun sequence.
ACCESSION   DBNGOH010001176 DBNGOH010000000
VERSION     DBNGOH010001176.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3004)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3004)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3004
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3845564"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            87..1466
                     /gene="tig"
                     /locus_tag="AC159P_22780"
     CDS             87..1466
                     /gene="tig"
                     /locus_tag="AC159P_22780"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HIR3698326.1"
                     /translation="MKTEIVDVSPTRKEIKIELMPEDVRGEFERVSQDFARHAAVPGF
                     RKGHTPVAVVRTRFKKEIRSEVLQKVVPTVVSRAIEENGLIVIGEPELHLDNAGLDKF
                     GQEPFSLHAHVEVLPDVELGEYKGLAAARRTRPVNEETVARVIEDLRESSASLQPVED
                     RAAEEGDTVTVTFKGRYVEPPSDEEINAEDVDVVLGGPGVLQEFTDQLTGKRADEVVT
                     FAVKYPEDFNAKGLAGKEIEYTATVTAVRRKEVPALDDEWVKSLGEEEVDSVESLRAR
                     VHENLTKHAAHESEHRLRDDVLARLVEAHQFEVPETLVSYQANQLMQNTVRDMMRRGV
                     DPRSPEFDWDALREMVSARAHEDLRASMLLDRVAEAEGLEVTDEEVEAEIDSMIEQSG
                     RPAAEVRAALTKEGGERSIADRLRNRKALDFLVAHAAVSDEEWRDEEKPEEKDAEAAG
                     EAREQSPEA"
     gene            1619..2242
                     /locus_tag="AC159P_22785"
     CDS             1619..2242
                     /locus_tag="AC159P_22785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012098225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HIR3698327.1"
                     /translation="MIEKLTSDERQYIPNPTVFEQTPRGERYSDVYSRLLRDNIVFLG
                     TPIDDTVANLIVAQLLFLEAEDPEKDINLYINSPGGSITSGMAIYDTMQFIRPDVVTI
                     CLGQCASFAALLLAAGAKGKRFALPNSRILIHQPHVQGGIGGQASDIKIQAEEIIRMR
                     ELTSTILAKHTGQPFETIERDVERDRIMSAQQSKEYGLIDEVITHRQ"
     gene            2484..>3004
                     /locus_tag="AC159P_22790"
     CDS             2484..>3004
                     /locus_tag="AC159P_22790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011938809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClpX C4-type zinc finger protein"
                     /protein_id="HIR3698328.1"
                     /translation="MVRRTDDTLRCSFCGKSQNEVKKLIAGPTVYICNECIDICNEII
                     TDDQQAEAVASRPPLPKPAEIKSFLDEYVIGQEETKKRLAVAVYQHYKRIEISRRRAD
                     IEIQKSNILLIGPTGTGKTLLAQTLARILSVPFTIVDATTLTEAGYVGEDVENIILKL
                     LQAANGDVERAQQ"
BASE COUNT          605 a          994 c          967 g          438 t
ORIGIN      
        1 aggccagttc aagaaccgaa agttttcctg acctctgacc tctggcctct ggcctctata
       61 cttttgaaag cgggattgga ttcagtatga agacggaaat cgttgacgtt tcgccgacgc
      121 ggaaagagat caagatcgag ctgatgcccg aggacgtgcg cggcgagttc gagcgcgtga
      181 gccaggactt cgcgcgccac gccgccgtgc cgggcttccg caaggggcac acgcccgtcg
      241 cggtcgtgcg cacgcgcttc aagaaggaga ttcgcagcga ggtgctgcaa aaggtcgtgc
      301 cgacggtcgt cagccgcgcc atcgaggaga acggcctcat cgtcatcggc gagccggagc
      361 tgcacctcga caacgctggg ctagacaagt tcgggcagga gccgttctcg ctgcacgcgc
      421 acgtcgaggt gctgcccgac gtggaactcg gcgagtacaa ggggctggcc gccgcgcgcc
      481 gcacgcgccc cgtcaacgaa gagaccgtgg cgcgcgtcat cgaagatttg cgcgagtctt
      541 cggcctcgct ccagccggtc gaggatcgcg ccgccgaaga gggcgacacc gtcaccgtca
      601 ccttcaaagg ccgctacgtc gagccgcctt cggacgagga gatcaacgcc gaggacgtgg
      661 acgtggtcct gggcgggccg ggcgtgctgc aagagttcac ggatcagttg accggcaagc
      721 gcgccgacga ggtcgtgacc ttcgccgtca agtaccccga ggacttcaac gccaagggtt
      781 tggccgggaa ggagatcgag tacacggcga ccgtcaccgc cgtcaggcgc aaggaggtgc
      841 ccgcgctcga cgacgagtgg gtgaagagcc tcggcgaaga ggaggtggat tcggtcgaga
      901 gcctgcgcgc ccgcgtccac gagaatctga cgaagcacgc cgcgcacgag tccgagcacc
      961 gcctgcgcga cgacgtgctg gcgcggctcg tcgaggcgca ccagttcgag gtgcccgaga
     1021 cgctcgtcag ctaccaggcc aaccagctca tgcagaacac ggtgcgcgac atgatgcggc
     1081 gcggcgtcga tccgcgctcg cccgagttcg actgggacgc gctgcgcgag atggtgagcg
     1141 cccgcgcgca cgaagacctg cgcgcctcga tgctgctcga ccgcgtcgcc gaggccgagg
     1201 gtctggaggt cacggacgag gaggtcgagg cggaaatcga ctcgatgatc gagcagtcgg
     1261 ggcggcccgc cgcagaggtg cgcgctgcct tgacaaagga ggggggcgaa cgtagcatcg
     1321 ccgacaggtt acgcaatcgt aaggcgctcg acttcctcgt cgcgcacgcc gccgtcagcg
     1381 acgaagagtg gcgcgacgaa gagaagccgg aagagaaaga cgccgaggcg gcgggcgagg
     1441 cccgagagca gtcgccggaa gcctgagata catcacggag atttggaacc tcaaatttga
     1501 aattcaaatg agagctttca agtctccttt tcatttgtac ggggtcggca tcggcagcgc
     1561 gcgaagcgcg ccggcgtgcg gtcggagaga ggttcataag atgattcgcg tccctaacat
     1621 gatcgagaag ttgaccagcg acgagcggca gtacatcccc aacccgaccg tcttcgagca
     1681 gacgccgcgc ggcgagcgct actcggacgt gtactcgcgc ctgctgcgcg acaacatcgt
     1741 cttcctcggc acgccgatcg acgacacggt cgccaacctg atcgtcgcgc agctcctctt
     1801 cctcgaagcc gaagaccccg agaaggacat caacctctac atcaactcgc ccggcggctc
     1861 gatcacctcg ggcatggcca tctacgacac gatgcagttc atccgccccg acgtggtgac
     1921 gatctgcctg ggtcagtgcg cctcgttcgc ggcgctgctg ctggcggcgg gcgccaaggg
     1981 caagcggttc gcgctgccga actcgcgcat cctcatccac cagccgcacg tgcagggcgg
     2041 catcgggggc caggcgagcg acatcaagat tcaggccgag gagatcatcc gcatgcgcga
     2101 gctgacctcg accatcctcg ccaagcacac gggccagccc ttcgagacca tcgagcgcga
     2161 cgtggagcgc gaccgcatca tgtccgcgca gcagtccaaa gagtacggcc tcatcgacga
     2221 ggtcatcacg caccgccagt gacctcacgc gcccggcggc gagagacgac aatatccccc
     2281 ggcgcacgtt ctgtgataga gtagctgagc gcggcgcggc cagacccgcc gccgcgcccc
     2341 cggcccgacg ctcgttgcag cgagccttag ttgacacaac agacaggtcg atagtccggg
     2401 cgcgcgccga acggaagcgc gtgcctcaac ccccccaggg ttttcacgca ggttaagccg
     2461 aacggagttt ccctcaaacg cctatggtac gccgaactga cgacacgctg cgctgctcct
     2521 tctgcgggaa gtcgcagaac gaggtcaaga agctcatcgc ggggccgacc gtctacatct
     2581 gcaacgagtg catcgacatc tgcaacgaga tcatcaccga cgaccagcag gccgaggccg
     2641 tcgcctctcg tcccccgctc ccgaagccgg ccgagatcaa gtccttcctc gacgagtacg
     2701 tcatcgggca ggaggagacc aagaagcgcc tggccgtcgc cgtctaccag cactacaagc
     2761 gcatcgaaat ctcgcgccgc cgcgccgaca tcgaaatcca gaagtcgaac atcctgctca
     2821 tcggcccgac cggcaccggc aagacccttc tcgcccagac gctcgcgcgc atcctctcgg
     2881 tccccttcac catcgtcgat gcgacgaccc tgaccgaggc cggctacgtc ggcgaggacg
     2941 tggagaacat catcctcaag ctgctgcaag ccgccaacgg cgacgtggag cgcgcgcagc
     3001 agga
//
LOCUS       DBNGOH010001177         5371 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_364367, whole genome shotgun sequence.
ACCESSION   DBNGOH010001177 DBNGOH010000000
VERSION     DBNGOH010001177.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5371)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5371)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5371
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_364367"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1037)
                     /gene="xrt"
                     /locus_tag="AC159P_22795"
     CDS             complement(<1..1037)
                     /gene="xrt"
                     /locus_tag="AC159P_22795"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:NF021254.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase"
                     /protein_id="HIR3698329.1"
                     /translation="MDRLLPRNLWKPILIAAAIAFAYAGVIARLGRLWWDDENYSHGL
                     LIPFVIGYILWTERGRLAAEPKRPSFWWGGAAIVCGLFGLWAGTAGAELFVQRTSLVL
                     VLAGVAVYFWGFRLLKLVLVPLFLLLLAIPIPTIVFNKVAFPLQLFASRVAVWTMRQF
                     DIPVLRQGNVIELMPLNSMTTKKLEVVEACSGIRSLMTLVTLAVVFAYFTSPARGGGD
                     GKGGGRSDFSLRSWAFWRSFIIVVSAVPIAIVTNAARVSGTGVLARYYGTEVADGFFH
                     SFSGWVVYVVAFLLLFAVGWLLDRVGPGGRGGRRSGDKEKTTKTTSTADADETGVVGG
                     APSAAMSSVGAT"
     gene            complement(1224..4457)
                     /locus_tag="AC159P_22800"
     CDS             complement(1224..4457)
                     /locus_tag="AC159P_22800"
                     /inference="COORDINATES: protein motif:HMM:NF037015.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4214 domain-containing protein"
                     /protein_id="HIR3698330.1"
                     /translation="MGPQLRSGNALNPVPWFDSNAVTKGIEHGEAFPAAPPLSAPLAG
                     TVSVTQGSRVVTGTGTRFLTDFSGGGALGYHVLIRDAEGSMRDYILASVESDTQLTVS
                     VAWQQPSASGRQVSKATAEELDAYVNKNYYDQGLVQYSNYYRTGDARFLAYARKITDS
                     WWKLPAFAEGRATVDDSISPRNSSLAGLMMRALDGRPEMWPWITEYVRTQHYLWVERP
                     LNLWYRPLTDGQIAGTEPRQEGGQTVTKPAGFYFGVRDGGYMLLHAANLARVHPDPAV
                     RAELRAKVLDVAVNYYGRLQRLQSNDGGFYFYLDGMEGLTTQPFQEGILGEGLVAVHR
                     LTGDERVRVSILKLAEHFYNRAYNPVGYRAMYYFVGGGWRNEDGSWTSCETGCGGASN
                     PWPPANSGIVSEARQLNPTAVHVFGYAFAVSGDARFRTWGDEVFDSSFSGTDGYRSLA
                     WGRPKEYDECYRSAGRYLAWRLSDGAPPPVPSPTPTPSATPTPTPTPGATPAPSPTPV
                     QSGVLSGRMLSGSAPLPGRRVLLYGADGVDPIGAASTDSQGRYGFETNAGQRVIIRPE
                     PAADLAFSPAQYDLTVSGTRSDLDFVSGSSPTPTPTPAPTPTPTPTPTPTPTPTPGLL
                     TTNISSPTGGAQYNAPANITISASASAASGQTITRVDFYAGTTMIGTDTTAPYSVVWG
                     DVAGGNYVLTSKATDNSGRSATSSGVQVQIKRSSGSVNRAKKNAQTVSNQLAETLTIT
                     ASGSTDSTEAASSTTTQLSRVVSDIQTAYNDFLAERGSYAAAGKIDAELQNALNHALL
                     ANSSAGGGPSNTVKVNLRAAIDYLELANVHLLHGDVPNPAEVTEFFVRQNYVDFLNRE
                     PDEEGLAYWAGEIDRCGADAQCRQVRRINVSAAFFLSIESQETGVLVHRLYKASYGSA
                     PTLAEFSPDVEEIARGIIVGAPGWNQKLEQNKSEFYRRWVRRARFAARYANLTNAQYV
                     DALIANAGVAVSQEVRDSWVAALDAGQPRDYALRRMVEHGDFVRGEYTTAFVMMQYMG
                     YLRRDPDPGGFNFWLTKLNEYGGDYNRAEMVRAFIESIEYNDRFRNR"
     regulatory      complement(5033..5145)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
BASE COUNT          893 a         1728 c         1896 g          854 t
ORIGIN      
        1 gtcgcgccga ctgaggacat cgccgccgaa ggcgcgccgc cgaccacgcc cgtctcgtcg
       61 gcgtcggcgg tgctcgtcgt cttcgtcgtc ttctctttat ccccgctgcg gcgcccgccg
      121 cgaccgcccg gccccacgcg gtcgagcagc cacccgacgg cgaagagcag caggaaggcg
      181 acgacgtaga cgacccagcc cgagaacgag tggaagaagc cgtcggccac ctccgtcccg
      241 tagtaacgcg cgagcacgcc ggtgccgctg acgcgcgcgg cgttggtcac gatggcgatg
      301 ggcaccgccg agacgacgat gatgaacgag cgccagaagg cccaactgcg caggctgaaa
      361 tcggagcgcc cgccgccctt cccgtccccg ccgccgcgcg cgggcgacgt gaagtaggcg
      421 aagacgacgg cgagcgtcac gagcgtcatc aagctgcgga tgccgctaca ggcttcgacc
      481 acttcgagct tcttggtcgt catcgagttg agcggcatca gctcgatgac gttgccctgc
      541 ctgagcacgg ggatgtcgaa ctgccgcatc gtccagaccg cgacgcgcga ggcgaagagc
      601 tgcaaaggga aggcgacctt gttgaagacg atggtcggga tcgggatggc caggagcagc
      661 aggaagagcg gcacgagcac gagcttcagg agccggaagc cccagaagta gacggccacg
      721 cccgccagca ccagcacgag cgacgtgcgc tgcacgaaca gctcggcgcc cgccgtgccc
      781 gcccacagcc cgaacagccc gcagacgatg gccgccccgc cccaccagaa cgaagggcgc
      841 ttcggctccg ccgcgagccg cccgcgctcc gtccacagga tgtagccgat gacgaacggg
      901 atgagcaggc cgtgcgagta gttctcgtcg tcccaccaga gccgaccgag acgcgcgatc
      961 acgcccgcgt aggcgaacgc aatcgcggcg gcgatcagga ttggcttcca gaggttccga
     1021 gggaggaggc gatccattcg agtttactgc ggcttacaca acagaacagt atttgtcagt
     1081 ggggagtgac aggcgcgggg gcacgtcacc cgagcgccgt ggaagcttaa cgcaaaccac
     1141 gacgcgcaag caaacgcgaa gcgtaaaagc ctctgccccg acggtcactt gacgatcgag
     1201 tggccgcgcg ccgtctgcgc ccgtcacctg ttccggaagc ggtcgttgta ctcgatggac
     1261 tcgatgaagg ccctgaccat ctcggcgcgg ttgtagtcgc cgccgtactc gttgagcttc
     1321 gtcagccaga agttgaagcc gcccgggtcc gggtcgcgcc tgaggtagcc catgtactgc
     1381 atcatcacga aggccgtggt gtactcgccg cgcacgaagt cgccgtgctc gaccatccgc
     1441 cgcagcgcgt agtcccgcgg ctggcccgcg tccagcgccg ccacccacga gtcgcgcacc
     1501 tcttggctca ccgccacgcc cgcgttggcg atcagcgcgt ccacgtactg cgcgttcgtc
     1561 agattggcgt accgcgccgc gaagcgcgcg cgccgcaccc accgacggta gaactcggac
     1621 ttgttctgtt cgagcttctg gttccagccg ggcgcgccga cgatgatgcc gcgcgcgatc
     1681 tcctcgacgt cgggcgagaa ctcggcgagc gtcggcgcgc tcccgtaact cgccttgtaa
     1741 aggcggtgga cgagcacgcc cgtctcctga gactcgatgg agaggaagaa ggcggcgctg
     1801 acgttgatgc gcctgacctg ccgacactgc gcgtccgcgc cgcagcggtc gatctcgccc
     1861 gcccagtagg ccagcccctc ctcgtccggc tcgcggttca ggaagtccac gtagttctgc
     1921 cggacgaaga actcggtgac ctccgccggg ttgggaacgt cgccgtggag gaggtgcacg
     1981 ttggccagtt cgaggtagtc gatggccgcc cggaggttga ccttgacggt attgctcggc
     2041 ccgccgccgg cggagctgtt ggcgagcagc gcgtggttca gggcgttctg tagctcggcg
     2101 tcgatcttgc ccgccgcggc gtaggagccg cgctcggcga ggaagtcgtt ataggccgtc
     2161 tggatgtcgg agacgactct ggagagctgc gtggtggtcg agcttgcggc ctccgtcgag
     2221 tcggtgctcc ccgaagcggt gatcgtcaga gtttcggcga gttgattact cacggtctgc
     2281 gcgttcttct tcgccctgtt cacagacccg gaagagcgtt tgatctgcac ctgaacgcca
     2341 ctcgacgtgg cgctcctgcc ggagttgtcc gtcgccttgc tggtcaggac gtagttgccg
     2401 cccgcgacgt cgccccacac gacgctgtag ggtgcggtcg tgtcggttcc gatcatcgtc
     2461 gtccccgcgt aaaaatccac cctggtgatg gtctggcccg aggcggcgct ggcgctggcg
     2521 ctgattgtga tgttagcggg cgcgttgtac tgcgcgccgc cggtcgggct ggaaatgttc
     2581 gtcgtcagga gaccgggcgt cggcgtcggc gtgggtgttg gagtaggcgt cggagtaggc
     2641 gtcggagcgg gcgtcggcgt cggcgtcggg gaagaaccgg agacgaagtc gaggtcgctg
     2701 cgagtgccgg agacggtcag gtcgtactgg gcaggggaga aggcaaggtc ggcggcgggc
     2761 tcggggcgga tgatgacgcg ctggccggcg ttggtctcga agccgtagcg gccctgcgag
     2821 tcggtggagg cggcgccgat ggggtctacg ccgtcggcgc cgtaaagcag gacgcggcgg
     2881 ccaggcagcg gcgccgagcc ggagagcatc cgcccggaga gcacgccgct ttgaacgggg
     2941 gtcggtgaag gcgccggggt ggcgccgggc gtcggcgtgg gcgtcggcgt ggccgaaggc
     3001 gtcggcgtgg gcgatggcac agggggcggc gcgccgtcgc tcagccgcca cgccagatac
     3061 ctgcccgcgg agcggtaaca ttcgtcgtat tccttcgggc gcccccaggc gaggctgcgg
     3121 tagccgtcgg tgccgctgaa ggaggagtcg aagacttcat cgccccacgt ccggaaccgc
     3181 gcgtcgcccg agacggcgaa ggcgtagccg aagacgtgaa ccgccgtcgg gttcaactgt
     3241 ctcgcctcgg agacgatgcc cgagttggcc gggggccacg ggttcgacgc gccgccgcac
     3301 cccgtctcgc acgacgtcca agagccgtcc tcgttccgcc agcccccgcc cacgaagtaa
     3361 tacatcgcgc ggtagccgac ggggttgtag gcgcggttgt agaagtgctc ggcgagtttg
     3421 aggatcgaga cgcgcacgcg ctcgtcgccg gtcaggcggt gcaccgcgac gagtccctcg
     3481 ccgaggatgc cctcctgaaa aggttgggtt gtcaacccct ccatgccgtc gaggtagaag
     3541 tagaagcccc catcgttcga ctggaggcgc tggagccgac cgtagtagtt gaccgcgacg
     3601 tccaaaactt tggcgcgcaa ctcggcgcgc accgccgggt cggggtggac gcgggcgagg
     3661 ttcgcggcgt gcagcagcat gtagccgccg tcgcgcacac cgaagtagaa gcccgcgggc
     3721 ttcgtgaccg tctgcccgcc ctcctggcgg ggttcggtgc cggcgatttg gccgtcggtg
     3781 agaggccgat accagaggtt gaggggccgc tccacccaca gatagtgctg cgtgcggacg
     3841 tactccgtaa tccagggcca catctccggc cgtccgtcga gcgcccgcat catgaggccc
     3901 gcgagcgagg agttgcgcgg gctgatgctg tcatcgaccg tcgccctccc ctcggcgaag
     3961 gccggcagtt tccaccagga atcggtgatc ttgcgagcgt aggcgaggaa gcgcgcgtcg
     4021 ccggtgcggt agtagttgct gtactggaca agcccctggt cgtagtagtt cttgttgacg
     4081 taggcgtcca actcctcagc cgtggccttc gacacctggc ggcccgaggc cgagggctgc
     4141 tgccacgcga ccgagacggt gagctgcgtg tccgactcga cgctcgccag gatgtagtcg
     4201 cgcatgctcc cctctgcgtc gcggatgagc acgtggtagc cgagcgcgcc gccgcccgag
     4261 aagtcggtca ggaagcgcgt gccggtgccg gtcacgacgc ggctgccctg cgtcacgctc
     4321 accgtgccgg ccagcggcgc gctgagcggc ggggcggcgg ggaaggcctc cccgtgctcg
     4381 atgcctttgg tgacggcgtt cgagtcgaac cacgggacgg ggttgagcgc gttcccgctc
     4441 ctgagctggg ggcccatgat gaactccacc tccggcgacg ggaaggaaac ataatcagca
     4501 ctcgtcacct gcgccacgga cccaccggcg ggagcgaggg cggtggctcg ggccaaaggc
     4561 aacgcgccgg acaagggcgt caagaccatc agggcggtgg cgagcaggct gaacagcttc
     4621 gaaagtccgg cggcgttgtg tgtaacccgg gcgcaggtgc ggttggacat cgtcagaacc
     4681 tttcatttcg gggtacgccc cgcggcgggg cgcgaaatgg gggaggaagg cgcacgatga
     4741 ccggccctga gcgcggccac gggccgcgcc tagacaaggc ttcgccactg tcgtctcggc
     4801 gcgaccgggg tcgccgtcgg cgctgactgt ggatatttac ggagaggaaa aggtacgacg
     4861 gagggttggg aaccgacgac acgggggcaa tgctgacctg tggttccagc ctgggcgcct
     4921 tcggcggacg gcgcgagcgc ctccgggact gcgggttgct taaccccgcc cccggcctgc
     4981 gcccacaccc ctgccgcgcg tgcgaaagga gccctgacta tctccgatcg ccttcggcaa
     5041 ccagaccttc tccccgcgtc ccgcgccccc tcgaaagaag ggccgggccg gggaagactc
     5101 ttggctttgc gtccctgcct cgcgacaggt ttgcctttat cgtgtgcaat cgtctcgttc
     5161 ccaaagagca tggcggcaga aagcttgcct gccgcggcta tacccataat gcaaagatgg
     5221 cggatgtttg tcaaggatat tataaacaca aaaaggttaa acaaaatggc gtgtaaaaat
     5281 ttcatcctcc gggccgctga aaaagcggcg ccggttgcag atcgaacggt ttgagcggcg
     5341 ctggatgttc tgcggaggcc gagccttagg c
//
LOCUS       DBNGOH010001178         3301 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_547275, whole genome shotgun sequence.
ACCESSION   DBNGOH010001178 DBNGOH010000000
VERSION     DBNGOH010001178.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3301)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3301)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3301
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_547275"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            222..2858
                     /locus_tag="AC159P_22805"
     CDS             222..2858
                     /locus_tag="AC159P_22805"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HIR3698331.1"
                     /translation="MKLAFFTPLNPQPSGISDYSEELLPYLAARAEVDLFVDGFAPTN
                     RELTARLRVHDYRRDPAALGLLDRYDAIVYHVGNDHRYHAGILEVMRARPGVAVFHDY
                     ALQDFFLGLARARGDARLYLDEVEACHGAAARREAEEALARGGTPAGVALPTEFPLNC
                     RAARAAEGIIVHSEWSRARFAEVAPGVPVARINMPVRPDAASRPPRDMADESGEAERE
                     VSIATFGLITPGKGIERTLRALAALTPRRRFRYTLVGAPNPYFDVAGFVRESGLSGRV
                     AVTGHVTLAEFERHITETDIAVNIRERTVGETSASLCRIMAAGVAAVVSNVGWYAELP
                     PDAVVKIDLDRDADAMLEAYLDRLVEDAPLRRRIGANARRHVLAEHDIERSADAYVAF
                     IREVIAGRARRRFVGGVSAELTRLGARPSDEKLMRAVAAEVALLAPPATTHAARDEQH
                     TNGDGHLTFTNAGDDGDGEATPRDAQGRTPKIEGVNYKRAAREYLGRLSEERRHHLRT
                     KPFYNLAHKPAKYRNDGLDEDSHRHLCDFANMAVALALPPGSRVLDVGCGSGWLSEYF
                     ARLGYDVRGIDISPALVEMSRERVARVPYGVDHETPLRCTFEVHDIEAAPLGEKFDAV
                     VCYDALHHFEDERAVFRHLAAMLDVGGQLFILEGERPPALSASEEELRGVMREFGTLE
                     SPFDPSYLRALLDENGLAVVGDYVSVNGLFERALLEGDRLPLNVVATDYHYLICKKVS
                     AGARASTVPDSRQPGTLSAHIALLDPLPERFAPGVRIEARLAVTNAGDTLWLTGQSVR
                     AGLVMPALRIHDGEGRIVGERHGPLLARAVAPGETVALRVECNAPTRPGTYTLKIDLV
                     DQHVCWFEERGSAPLTLRFEVG"
     gene            2896..>3301
                     /locus_tag="AC159P_22810"
     CDS             2896..>3301
                     /locus_tag="AC159P_22810"
                     /inference="COORDINATES: protein motif:HMM:NF019615.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1698 domain-containing protein"
                     /protein_id="HIR3698332.1"
                     /translation="MTMTREEILSELERLQPWFHRIDLGGGLFTKTESVMGEPVDHPA
                     GPWQTIGKCVPADLAGKSVLDVGCNAGFYAFEAKRRGAARVLGVDGQRQHVRQALFVR
                     KALGMEGVEFRRMNVYELTRRNVGQFDITLALG"
BASE COUNT          529 a         1228 c         1092 g          452 t
ORIGIN      
        1 aggaggtgga agtgccgctc cagatcacgg cgccgcagga gcttggcgag tacgtcctcg
       61 aagttgacct catacaggag caggtggcat ggttcagcga caaggggtcg cccgccgcgc
      121 aggccaaagt ggcggtggtt cggtaacggc gggcggcgtg taaaggccga ccgattcgga
      181 gtgaacggcg gcggctgacc gagcgccgtc cgcatcgacg catgaagtta gcctttttca
      241 caccgcttaa cccgcagccg tccggcatct cggactacag cgaggagctg ctgccgtacc
      301 tcgcggcacg cgccgaagtc gatctcttcg tggacggctt cgcgccgacc aaccgcgagc
      361 tgacggcgcg cctgcgcgtc cacgactacc gccgcgaccc cgccgcgctc ggactgctcg
      421 accgatacga cgccatcgtc taccacgtcg gcaacgacca ccgctaccac gcgggcatcc
      481 tcgaagtgat gcgtgcgcgt ccgggcgtcg ccgtcttcca cgactacgcg ctgcaagact
      541 tcttcctcgg gctggcgcgc gcgcgcggcg acgcgcggct gtacctcgac gaggtcgaag
      601 cctgccacgg cgcggcggcg cggcgcgaag ccgaagaggc gctcgcgcgc ggcggcacgc
      661 ccgcgggcgt cgcgctgccc acggagttcc cgctcaactg ccgcgccgcg cgcgccgccg
      721 agggcatcat cgtccactcg gagtggagcc gcgcgcgctt cgcggaagta gcgcccggcg
      781 ttcccgtcgc gcgcatcaac atgccggtca ggccggacgc cgcgtcgcga ccgccgcgcg
      841 acatggctga cgaatccgga gaggccgaac gcgaagtctc gattgcgacg ttcggcctca
      901 tcactcccgg caagggcatc gagcgcacgc tgcgcgcgct cgccgcgctc acgccccggc
      961 gccgcttccg ctacacgctc gtcggcgcgc ccaaccctta cttcgacgtg gccgggttcg
     1021 tccgcgagag cggcctctcg ggccgcgtcg cggtcacggg ccacgtcacg ctcgccgagt
     1081 tcgagcggca catcacggag accgacatcg ccgtcaacat ccgcgagcgc accgtcggcg
     1141 agaccagcgc cagcctctgc cgcatcatgg ccgcgggcgt cgccgccgtc gtctcgaacg
     1201 tcggctggta cgccgagctg ccccccgacg cggtcgtcaa aatcgacctc gaccgcgacg
     1261 ctgacgcgat gctcgaagcc tacctcgacc gcctcgtcga agacgccccc ttgcgccgtc
     1321 gcatcggcgc caacgcgcgc cgccacgtgc tcgccgaaca cgacatcgaa cgcagcgccg
     1381 acgcctacgt cgccttcatc cgcgaagtga tcgcgggccg cgcgcgtcgg cgcttcgtcg
     1441 gcggcgtctc cgcagagctg acgcgactcg gcgcgcgccc ctccgacgaa aagctcatgc
     1501 gcgccgtcgc cgccgaggtc gcactgctcg cgccgcccgc gacgacgcac gccgcccgcg
     1561 acgaacagca cacgaacggc gacgggcacc tcactttcac gaacgcgggc gacgacggcg
     1621 acggcgaagc gacaccgcgc gacgcgcagg gtcggacgcc gaagatcgag ggcgtaaact
     1681 acaagcgcgc ggcgcgcgaa tacctcggca ggctgagcga ggagcgccgc caccacctgc
     1741 ggacgaagcc cttctacaac ctcgcgcaca agcccgcgaa gtaccgcaac gacgggctgg
     1801 acgaagactc gcaccgccac ctctgcgact tcgccaacat ggccgtcgcg ctcgccctcc
     1861 cgcccggctc gcgcgtcctc gacgtgggct gcggctccgg ctggctcagc gaatacttcg
     1921 cgcgcctcgg ctacgacgtg cgcggcatcg acatcagccc cgcgctcgtc gagatgtcgc
     1981 gcgagcgggt cgcgcgcgtg ccctacggcg tggatcacga gacgccgctc cgctgcacgt
     2041 tcgaggttca cgacatcgag gccgcgccgc tcggcgagaa gttcgacgcc gtcgtctgct
     2101 acgacgcgct ccaccacttc gaagacgagc gcgccgtctt ccgccacctc gccgcgatgc
     2161 tcgacgtggg cggccagctc ttcatcctcg aaggcgagcg cccgcccgcc ctgtcggcga
     2221 gcgaagaaga gctgcgcggt gtcatgcgcg agttcggcac gctcgaatcg ccgttcgacc
     2281 cgtcgtacct gcgcgcgctc ctcgacgaga acggcctcgc cgtcgtcggc gactacgtca
     2341 gcgtcaacgg cctcttcgag cgcgcgctgc tcgaaggcga ccgcctgccg ctcaacgtag
     2401 tggcgaccga ctaccactac ctcatctgca aaaaagtctc ggcgggcgcg cgcgcctcga
     2461 ccgtccccga cagccgccag cccggcacgc tcagcgcaca catcgcgctg ctcgacccgt
     2521 tgcccgaaag gttcgcgccc ggcgtacgga tcgaagcacg gctcgccgtc accaacgcgg
     2581 gcgacacgct ctggctcacg ggccagtcgg tgcgcgccgg tctcgtcatg cccgccctca
     2641 gaatccacga cggcgaaggc cgcatagtcg gcgaacgcca cggcccgctg ctcgcgcgcg
     2701 ccgtcgcgcc cggcgagacc gtcgcgctcc gcgtcgagtg caacgcgccg acgcgccccg
     2761 gcacatacac cttgaaaatc gacctcgtcg atcagcacgt ctgctggttc gaggagcgcg
     2821 gctccgcgcc gctcacttta cgattcgaag tcggataaac atgtcgatga atccggagtt
     2881 gggtagagca cgacgatgac gatgacgcgc gaagagattc tgtccgagct tgaaaggctc
     2941 cagccgtggt ttcaccgcat cgacctcggc ggcgggctgt tcacgaagac cgagagcgtg
     3001 atgggcgagc cggtcgatca cccggcgggg ccgtggcaga ccatcgggaa gtgcgtcccg
     3061 gcagacctcg cgggcaagtc ggtgctcgac gtggggtgca acgcgggctt ctacgcgttc
     3121 gaggccaagc ggcgcggggc ggcgcgcgtc ctcggtgtgg acgggcagcg gcagcacgtg
     3181 cggcaggcgc tgttcgtccg caaggcgctc ggcatggagg gcgtggagtt ccggcggatg
     3241 aacgtctacg agctgacgcg ccgcaacgtc gggcagttcg acatcacgct cgcgctcggc
     3301 c
//
LOCUS       DBNGOH010001179         4508 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3662311, whole genome shotgun sequence.
ACCESSION   DBNGOH010001179 DBNGOH010000000
VERSION     DBNGOH010001179.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4508)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4508)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4508
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3662311"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(32..4108)
                     /locus_tag="AC159P_22815"
     CDS             complement(32..4108)
                     /locus_tag="AC159P_22815"
                     /inference="COORDINATES: protein motif:HMM:NF019607.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HIR3698333.1"
                     /translation="MVKIKLLVVLVFVAGVGAILLFGNGGAGRRVHAFSAGPPAGYTR
                     APGEEPEACAECHVVENAGTGQLTLTVPPTYVPGQTYDITVRHTNTDQTRVRWGFQLT
                     ALDASDEKAGTLAPLDELTQVLDNQGPFPDRQYIEHTSQGTFFGQQNGAAWTFRWTAP
                     AENVGPVTFYAAGNQANGDSNTSGDNIYFTFVASTFTPPAPDFNVTITPETRIVTRGD
                     PAVFNVTVTPTGGFTGNVTLAVANPPANPGHAFDPVTLSITDANPKSSTLTWTTDNAT
                     PLGQFAFDVTATSGQRQRSAQGGFIIAGPDSANLTTVHTDSPDPAVVGENLSYRIEVT
                     NNGPAAATGVNVVDALPAGVRLVSATPTQGSCTGTTNIDCALGGLAVGASAVVTVVVV
                     PQSVGTLLNTAGATATQSDPFSIDNFKTAVTTVVAPSTGPGMLVPNLGVRTVVNGLDQ
                     PTSMAFIGPHDFLVLEKESGRVRRVTDGVVQGTVLDLAVNSASERGLHGITLHPNFAA
                     NGFVYLYWTESLTGADTTAIDSVPLLGNRIDRFTWNGSTLTQNRNIIRLRALQADAGQ
                     PPRGNHDGGVLRFGPDGKLYVVVGDLGRRGLLQNIAAGGPVPDDQFGGPEPDDAHLSG
                     VILRLNDDGTSPVDNPFFNAVTPFTGEAAANVKKIFAYGVRNSFGMDFDPFGGALWTQ
                     ENGDDAFDEINRVAPGFNGGWIQVMGPSSRVAEFKQIESSRPGGLQQNRWPPSNIADT
                     PAEALARLFQLPGAVYTEPAFSWKYAVAPSPIGFARAGLGSFSGGLFVGASRTTLLGG
                     YLFHLRLNAARDGFATGDPRLEDRVADNADKFDLAESESLLVGRDFGITTDIQTGSNG
                     NLYVVSLSNGAVYEVFARPTLHIASLTGAQETPPNSSQAYGTATLLLDPGEITARVAV
                     EFTGFPTPQTLAHIHGPAPAGQSAPPIIDLPVGSFSNHLVSLTPQQVSWLKAGLLYVN
                     LHSGAFPAGEIRGQLGLVPAASNVRFDIADPNVSEGAGFKTVNVVRTGDTSGAASVEY
                     ATSGGPASQRNDYTAARGTLTFAPGETSKSFDVLITDDAIQEGDEEIELLLTPLPGGN
                     AFADPDLASLVIKDNDASPPSSNPVDATAFFVRQHYHDFLSREPDSLGLQFWSNEIDS
                     CGADEQCREVKRVNVSAAFVLSIEFQETGYLAYRFYEAAFDRRLCYLEFIRDTQEIGR
                     GVEVGVGDWQARLEANKQAFAERFVERPVFKSFFDALSNAQYVDRLFQNAGVVPSQAE
                     RDALAGGLDSGTETRATVLRKVADNARFRALEFNRAFVLMQYFGYLRRNPDDPPDTNL
                     DGYDFWLAKLEQFNGDFVRAEMVRAFISSIEYRQRFGQ"
BASE COUNT          667 a         1338 c         1745 g          758 t
ORIGIN      
        1 cacccccgcc tttttatctt ttctttccgc gttactggcc gaagcgctgg cggtattcga
       61 tcgaggagat gaaggcgcgc accatctcgg cgcggacgaa gtcgccgttg aactgttcga
      121 gcttggcgag ccagaagtcg tagccgtcga ggttcgtgtc gggcgggtcg tccgggttgc
      181 gccggaggta gccgaagtat tgcatgagga cgaaggcgcg gttgaattcg agggcgcgga
      241 accgggcgtt gtccgcgacc ttgcggagca cggtggcgcg cgtctccgtg ccggagtcga
      301 gaccgcccgc gagcgcgtcg cgttcggcct gcgacgggac gacgcccgcg ttctggaaga
      361 gtctgtccac gtactgcgcg ttcgagagcg cgtcgaagaa gctcttgaag acgggtcgct
      421 cgacgaaacg ttcggcgaac gcctgtttgt tggcttcgag ccgcgcctgc cagtcgccga
      481 cgccgacctc gacgccgcgc ccgatctcct gcgtgtcgcg gatgaactca aggtagcaca
      541 ggcggcggtc gaacgcggcc tcgtagaagc ggtacgcgag atagcccgtc tcctgaaatt
      601 cgatggagag gacgaaggcg gcggagacgt tgacgcgttt gacctcgcgg cactgctcgt
      661 ccgcgccgca cgagtcgatc tcgttcgacc agaactgaag gccgagggag tccggctcgc
      721 ggctgaggaa gtcgtggtag tgctggcgga cgaagaaggc ggtcgcgtca acagggttgg
      781 aggacggcgg cgaggcgtcg ttgtctttga tgacgagcga ggcgaggtct gggtcggcga
      841 aggcgttccc gcccggcagc ggcgtgagca gaagctcgat ctcctcgtcg ccctcctgta
      901 tggcgtcgtc ggtgatgagc acgtcgaagc tcttcgaggt ctctccgggg gcgaaggtga
      961 gcgtgccgcg cgcggcggtg tagtcgttgc gctggctggc ggggccgccc gaggtggcgt
     1021 actcgacgga ggccgcgccg gaggtgtcgc cggtgcggac gacgttgacg gtcttgaagc
     1081 ccgcgccttc gctgacgttc gggtcggcga tgtcgaagcg gacgttggag gcggcgggga
     1141 cgaggccgag ctggccgcgt atctcgcccg ccgggaaggc gccgctgtgc aggttgacgt
     1201 agaggaggcc ggccttgagc cagctcacct gttgcggcgt gaggctgacg aggtggttcg
     1261 agaagctgcc cacgggcagg tcgatgatgg gcggcgcgct ctggcccgcg ggcgcggggc
     1321 cgtggatgtg cgcgagcgtc tgcggcgtcg ggaagccggt gaactcgacg gcgacgcgcg
     1381 cggtgatctc gccggggtcg agaagcagcg tggccgtacc gtaagcctgc gagctgttgg
     1441 gcggcgtctc ctgcgcgccg gtgaggctgg cgatgtggag cgtgggccgc gcgaagactt
     1501 cgtagaccgc gccgttcgag agcgagacga cgtagaggtt gccgttcgag ccggtctgga
     1561 tgtcggtcgt gatgccgaag tcgcgcccga cgagcaggct ctcgctctcc gcgaggtcga
     1621 acttgtcggc gttgtcggcg acgcggtctt cgaggcgcgg gtcgccggtg gcgaagccgt
     1681 cgcgggcggc gttgagcctg aggtggaaga ggtagccgcc gaggagcgtc gtgcgcgagg
     1741 cgccgacgaa gaggccgccg gagaatgagc cgaggcccgc gcgcgcgaag ccgatgggcg
     1801 agggcgcgac ggcgtatttc caactgaagg ccggctcggt gtagacggcg cccggcagtt
     1861 ggaagaggcg cgcgagcgcc tcggcgggcg tgtcggcgat gttcgagggc ggccagcggt
     1921 tctgttgcag gccgccgggg cggctcgact cgatctgctt gaactcggcg acgcgcgagg
     1981 acggccccat cacttgaatc cagccgccgt tgaagccggg cgcgacgcgg ttgatctcgt
     2041 cgaacgcgtc gtcgccgttc tcctgcgtcc agagcgcgcc gccaaagggg tcgaagtcca
     2101 tgccgaagct gttgcgcacg ccgtaggcga aaatcttctt gacgttggcg gcggcctcgc
     2161 cggtgaacgg cgtgacggcg ttgaagaagg ggttgtcaac agggctggtg ccgtcgtcgt
     2221 tgaggcggag gatgacgccc gagaggtgcg cgtcgtccgg ctcggggccg ccgaactggt
     2281 cgtcgggcac ggggccgccc gccgcgatgt tttgcagcag gccgcgccgc ccgaggtcgc
     2341 cgacgacgac gtagagcttg ccgtcggggc cgaagcggag cacgccgccg tcgtggttgc
     2401 cgcgcggcgg ctggcccgcg tccgcttgca gcgcgcgcag gcggatgatg ttccggttct
     2461 gcgtcagcgt cgagccgttc cacgtgaagc ggtcgatgcg attgccgagc agcggcaccg
     2521 agtcgatggc ggtggtgtcg gcgcccgtca agctctcagt ccagtagagg tagacgaagc
     2581 cgttcgcggc gaagttcggg tgcagagtga tgccgtgcag gccgcgctcc gaggcgctgt
     2641 tgaccgcgag gtcgagcacc gtgccctgca cgacgccgtc cgtcacgcgc cgaactctgc
     2701 cgctctcttt ttcgaggacg aggaagtcgt gcgggccgat gaaggccatg ctcgtcggct
     2761 ggtcgaggcc gttgacgacg gtgcggacgc cgaggttggg gacgagcata ccggggcctg
     2821 tgctcggggc gacgacggtc gtgacggcgg tcttgaagtt gtcgatggag aacgggtcgg
     2881 actgcgtcgc ggtcgcgccc gccgtgttga ggagcgtgcc gaccgactgc ggcacgacga
     2941 cgaccgtgac cacggcgctc gcgccgacgg cgaggccgcc gagcgcgcag tcgatgttcg
     3001 tcgtgcccgt gcaggagcct tgcgtcggcg tcgccgagac gaggcggacg cccgcgggca
     3061 gcgcgtccac gacgttgacg cccgtcgcgg cggcggggcc gttgttcgtg acttcgatgc
     3121 ggtacgagag gttctcgccg acgacggcgg ggtcgggcga gtccgtatgg acggtggtca
     3181 ggtttgccga gtcggggccg gcgatgatga agccgccctg cgcggagcgc tgtcgctggc
     3241 cgctcgtcgc cgtgacatcg aaggcgaatt ggccgagcgg cgtcgcgttg tcggtcgtcc
     3301 aggtgagcgt ggacgacttc gggttcgcgt ccgtgatgga gagggtcacg gggtcgaagg
     3361 cgtggcccgg gttggcgggc gggttcgcga cggcgagcgt gacgttgccg gtgaagccgc
     3421 ccgtcggcgt gacggtgacg ttgaagacgg cagggtcgcc gcgcgtgacg atgcgcgtct
     3481 cgggcgtgat ggtgacgttg aagtcgggcg cgggcggcgt gaaggtcgag gcgacgaagg
     3541 tgaagtagat gttgtcgccc gaggtgttgc tgtcgccgtt ggcctgattg cccgcggcgt
     3601 agaaggtgac cggccccacg ttttcggcgg gcgccgtcca gcggaacgtc caggccgcgc
     3661 cgttctgctg cccgaagaag gtgccctgcg aggtgtgctc gatatactgc cggtcgggga
     3721 acggcccctg attgtcgagc acctgcgtca gctcgtcgag cggcgcgagc gtgcccgcct
     3781 tctcgtcgct cgcgtcgagc gccgtgagct ggaagcccca gcggacgcgc gtctgatccg
     3841 tgttggtgtg cctgaccgtg atgtcgtagg tctggccggg cacgtaagtc ggcggcacgg
     3901 tcagggtcaa ctgccctgtg cccgcgttct cgacgacgtg gcactcggcg cacgcctccg
     3961 gctcttcgcc cggcgcgcgc gtgtagcccg cgggcggccc cgccgagaag gcgtggacgc
     4021 ggcggcccgc gccgccgttg ccgaagagca gaatcgcgcc gacgccggcg acgaagacga
     4081 ggacgacgag gagtttgatc ttgaccatga gcgaacagcc cttgaggctc cgggtcggtt
     4141 gagcggcagt cgcggttatt tttaacacag aggacacgga gggcacacag agttcacgga
     4201 gcaaaccata tcggaatcga gatttcgaag ttcatttcat gtctttcttc tccgtgaact
     4261 ctgcgtgccc tccgtgtcca ctgtgttgat aatcgactca cgtaatgctc ggctgccggg
     4321 taacgaaaga aacagcccga cgacgcatcg ccgggctgtc acagtgcaat cgaacatgac
     4381 tcccctcgcc cacgtcgcgc ccacctcaca tcatgaagcc gaggttcgcg gacgagaagc
     4441 ggtcgatgag cgggaagacc gccggcagat cgctcgtcgg gaggccgtac caggcggcga
     4501 gcgtggcg
//
LOCUS       DBNGOH010001180         8451 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1646810, whole genome shotgun sequence.
ACCESSION   DBNGOH010001180 DBNGOH010000000
VERSION     DBNGOH010001180.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8451)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8451)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8451
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1646810"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..606
                     /locus_tag="AC159P_22820"
     CDS             <1..606
                     /locus_tag="AC159P_22820"
                     /inference="COORDINATES: protein motif:HMM:NF024764.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3698334.1"
                     /translation="PRHAYALYQLGYLAYNRKDMDAAINALNRATLADAKIGNAWTLL
                     TYAYLNRAQAAGGTGAKADADYLAAVRASESLIKVSNDDTSNALHGQALIRAKQYPRA
                     ALVLERLAAKPEADGTTLYLLGFAHSRSKNFPKAAAALERAATKATDNAEIFRELGYV
                     YENLKQYAKALAAYERGLQIAPDDADFKESAERIRPFAKQP"
     gene            744..1265
                     /locus_tag="AC159P_22825"
     CDS             744..1265
                     /locus_tag="AC159P_22825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3698335.1"
                     /translation="MSNTKISRPQARGAAMWVVVVVAAFAALSVWAVGARAATWKGLE
                     PLTSKRADVERVLGAPVSDKLAQHGTLHFNVTGGTVTVQFVDAKFVAAKKLAPEAEGT
                     VLQIVLQHERATDTPESMSLGSNRDFERDEKGNVAVYRNLKEGIFYTFVDGKLKTTRY
                     AASTEQIVRAQKR"
     gene            1356..2570
                     /locus_tag="AC159P_22830"
     CDS             1356..2570
                     /locus_tag="AC159P_22830"
                     /inference="COORDINATES: protein motif:HMM:NF020933.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA double-strand break repair nuclease NurA"
                     /protein_id="HIR3698336.1"
                     /translation="MLYRGHLLSALDERRHDFALFERTLRDEVGEAARRLRALGAMSA
                     AAARDAVGGAAARVTCPSAELDARGGVVVPFAEEWRSHEEARGWARGVLEGRVTMAAD
                     GSQIFPGREVSMPVAAVQVASFENAHTLGGEYTKEISIRVVTPDELLAGDRAYESPEQ
                     VVGVRRFELEVETICGFLERKRNWRARGERAPVAFLDGSLLISSRRKSVEVMFSSRYA
                     EALVKLLRLSRETGVPVVGFIDQSYAPDLRDLLETLDADWRPQSVFDAQILRAADDGA
                     PPLLDAWGDRTIFWHCRRANLADNFYDERGEPVVGFAYVQTTGDGHPARVEVPAWVYE
                     AGLLDEVLDAVRAECVVGNGYPYAVETADEAAVMTARDRQQFLRVMQDFAGRHSFAFR
                     VSRKAASKARRR"
     gene            2648..3220
                     /locus_tag="AC159P_22835"
     CDS             2648..3220
                     /locus_tag="AC159P_22835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HIR3698337.1"
                     /translation="MSESNFDAGFEGRGEDFVSRRGQEACPACGAQARRTDARFCATC
                     GRRLADEEYFPTDALLASYHQQRRRPSLDGAAAKKVGDRPRAVFYKRDARPRAARPDM
                     FSDRHGNNAAQTAMAFVVYALVPYLGILFCPGALLMGGVGLLRARRAPHVGGHRASLA
                     SIALGLLLFVAHLLLWWILFKVPEWTRQAF"
     gene            3350..3970
                     /locus_tag="AC159P_22840"
     CDS             3350..3970
                     /locus_tag="AC159P_22840"
                     /inference="COORDINATES: protein motif:HMM:NF053366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF8166 family protein"
                     /protein_id="HIR3698338.1"
                     /translation="MTRIAKIVKSNSHVDYVGRVIDRLDAAEPPTGEDYGFAQFVAVP
                     VAEGVEVIGVVYDTQLANPEYGSYGPRLSSRTELSVLSPDYLNEQGVLLGILLVGWRE
                     RRGAGGDGGEWEGRHEVPRRVIPVGQDVYALGDREVQDFHRGEGGAVQLRYYSQVITH
                     AGAFAVPLVEAVIAQLEPACAPAERQRLCVLKKSLSWQRTLGGVRL"
     gene            4080..4694
                     /locus_tag="AC159P_22845"
     CDS             4080..4694
                     /locus_tag="AC159P_22845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3698339.1"
                     /translation="MKNTRRLLAALVAGIFLAAAGAQAQRTRPVSAPAPEKTEAKPPV
                     PGVTANVPPAPQTVKVKYEGGVFGYTKKMDGTLTFDDQNRRLVFRDQKTQQEKISINY
                     DAIASAFADTQSKRPIAADVLGRASIFTLPALLIKKKFRYLTLQFNDEHSNATGITSF
                     KLENKEILASVLNTLAQKAGMTQRGEIFVRNKSAAAKTDAGTPN"
     gene            complement(4772..7726)
                     /locus_tag="AC159P_22850"
     CDS             complement(4772..7726)
                     /locus_tag="AC159P_22850"
                     /inference="COORDINATES: protein motif:HMM:NF037202.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type ISP restriction/modification enzyme"
                     /protein_id="HIR3698340.1"
                     /translation="MSRVENYMLSSVPLDAPEALARFLASYARDAKERVGCEPNASQS
                     LKNVREALEQALGLKFEGAQGEQFFRSTLVQTLFYGVFSAWVLWSKSDRGARRRDEFH
                     WHDATWTLHVPAIRTLFEQVVTPSNVRGLGLEEVLDRTADTLNRVETEKFFERFEREH
                     AVQYFYEPFLEAFDPELRKHLGVWYTPGEIVRYMVERTDRALREELRIENGLADERVY
                     VLDPCCGTGAYLVEVLRRIKRTIDEQGGDAPAANDLKRAALGRVFGFEILPAPFVVAH
                     LQLGLLLQTEGSPLAEGERVGVYLTNALTGWEPPRDPKKQLPFAFHALEEERAAAEHV
                     KRETPILVVIGNPPYNAFAGTSAEEEGDLVDVYKQGLSKEWGIKKFNLDDLYVRFFRL
                     AERRIAEQTGRGIVCFISNFSYLSDPSFVVMRQRFLSEFDKLWFDSLNGDSRETGKLT
                     PEGKPDPSVFSTRYNREGIRVGTAIGLLARGEERAAQPQVRTRELWGVTKREDLFAGL
                     DAEDFDAHYAPAQPDATNRYSFRPSAVGAHYLAWPKLTELSEVRSDGFFEKRGGALID
                     IDRGALEKRVGMYFDKSVDWETLKGLNTGLTRDAARFDAKMARVKVTSAETYEPSRLR
                     RYAVRPFDTRWAYYSPTRPLWNEPRPILWAQNRIKNGFIISRLKSEKNPEGSPIHFST
                     TLVDGQAIAVNPFAIPIRLCTSTGKMSRKDDKQSSLLDHAGTAEKETTANLSPRARAY
                     LAALGVGEVDGDAEAAALVWYHALAVGHAHAYLAENADGIRADFPRVPLPAARGLLES
                     SAALGRQVARLLDTETPVPGVTGGAIRPELRAIAVLRRADGAHALDPRAGDLELRAGW
                     GHRGKAGVVMPGKGRARTRDYAAPEREAPPTVAHALGAETYDVFLNDAAYWSNVPALV
                     WDYTVGGYQVIKKWLSYREHDILGRSLTPEEAREVTQTARRIAAVLLLTTRLDENYLR
                     VKADSYDWKATA"
     gene            complement(7838..>8451)
                     /locus_tag="AC159P_22855"
     CDS             complement(7838..>8451)
                     /locus_tag="AC159P_22855"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017914893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HIR3698341.1"
                     /translation="HLVKENVDRQLETCQEAPFYTLGPLATDIAPGYDHITSAIGAAM
                     IGWFGTAMLCYVTPKEHLGLPNKQDVKDGVIAYKIAAHAADLAKGHPGAQLRDNALSK
                     ARFEFRWEDQFNLSLDPEVAREYHDETLPQEGAKLAHFCSMCGPHFCSMKITQEVRDY
                     AAKKALDEQSALGAGLKEKAAEFVAAGAELYQGEPPAGARREH"
BASE COUNT         1376 a         2910 c         2778 g         1387 t
ORIGIN      
        1 ccccgccacg cctacgcgct ctaccagctc ggctacctcg cctacaaccg caaggacatg
       61 gacgcggcca tcaacgccct gaaccgcgcg acgctcgccg acgcgaagat cggcaacgcg
      121 tggacgctgc tgacctacgc ctacctgaac cgcgcgcagg ccgccggcgg caccggcgcg
      181 aaggccgacg ccgactacct cgccgcggtg cgcgccagcg agtctctcat caaggtctcg
      241 aacgacgaca cgtcgaacgc gctccacggg caggcgctca ttcgcgccaa gcagtacccg
      301 cgcgccgccc tcgtcctcga acgcctcgcc gccaagcccg aggccgacgg caccacgctc
      361 tacctgctcg gcttcgcgca ctcgcgctcg aagaacttcc cgaaggccgc cgccgcgctc
      421 gaacgcgccg ccaccaaagc gaccgacaac gccgaaatct tccgcgagct cggctacgtc
      481 tacgagaacc tcaagcagta cgccaaagcc ctcgccgcct acgagcgcgg cctccagatc
      541 gcgcccgacg acgccgactt caaagagtcc gccgagcgca tccggccctt cgcgaaacag
      601 ccgtgaatgg tgaatagtga atggtaaatg gtgaatagaa aaagcggccc gccctctcac
      661 gcgggccgct tacttttcga cgcccgacgc cggtcacgat ttacctattt actattcacc
      721 attcaccatt taccattcac ggcatgagca acacgaaaat ttctcgcccg caggcgcggg
      781 gcgcggcaat gtgggtggtc gtcgtcgtcg cggctttcgc ggcgctgtcg gtgtgggccg
      841 tcggcgcgcg ggccgcgacg tggaaggggt tggagccgct cacgtcgaag cgcgccgacg
      901 tggagcgcgt gctcggcgcg ccggtctcgg acaagctggc ccagcacggc acgctgcact
      961 tcaacgtgac gggcggcacc gtcaccgtcc agttcgtgga cgccaagttc gtcgccgcga
     1021 agaaactcgc gcccgaggcc gaaggcaccg tcctccagat cgtcctccag cacgagcgcg
     1081 ccacagacac gcccgagtcg atgagcctcg gctcgaacag agacttcgag cgcgacgaga
     1141 agggcaacgt cgccgtctac cgcaacctga aggaaggcat cttctacacc ttcgttgacg
     1201 gcaagctcaa gaccacgcgc tacgccgcct ccaccgagca gatcgtccgc gcgcaaaaac
     1261 gataaacaaa agtgatgagg actgaggact gaggactgag taatggcaac ctgtcttccg
     1321 ctcagtcctc agtcctcagt cctcagtcct tttttatgtt gtaccgcggg catctgttga
     1381 gcgcgctcga cgagcggcgc cacgacttcg cactgttcga gcgcacgctg cgcgacgagg
     1441 tgggcgaggc ggcgcgtcgt ctgcgcgcgc tcggcgcgat gagtgccgcc gcggcgcgcg
     1501 acgccgtggg cggcgcggcg gcgcgggtga cttgcccttc ggcggagctg gacgcgcggg
     1561 gcggcgtggt cgtgccgttc gccgaagagt ggcgctcgca cgaggaggcg cgcggctggg
     1621 cgcgcggcgt gttggagggg cgcgtgacga tggccgcgga cgggagccag atttttccgg
     1681 gccgcgaggt gtcgatgccc gtggcggcgg tgcaggtcgc ctcgttcgag aacgcgcaca
     1741 cgctcggcgg cgagtacacg aaggagatca gcatccgcgt cgtcacgccg gacgagctgc
     1801 tggcgggcga ccgcgcctac gaaagcccgg agcaggtggt cggggtgcgc cgcttcgagc
     1861 tggaggtcga gaccatctgc ggcttcctcg aacggaagag gaattggcgc gctcgcggcg
     1921 agcgtgcgcc ggtcgccttc ctcgacggct cgctcctcat ctcctcgcgc cgcaagagcg
     1981 tcgaggtgat gttctccagc cgctacgccg aggcgctcgt caaactcctc cggctctcgc
     2041 gcgagaccgg ggtgccggtc gtcggcttca tcgaccagtc ctacgcgccc gatctgcgcg
     2101 acctgctcga aacgctcgac gccgactggc ggccccagtc cgtcttcgac gcccaaatcc
     2161 tgcgcgccgc ggacgacgga gcgccgccgc tgctcgacgc gtggggcgac cgcacgatct
     2221 tctggcactg tcgccgcgcc aacctcgccg acaatttcta cgacgagcgc ggcgaacccg
     2281 tcgtcggctt cgcctacgtg cagacgacgg gcgacggcca cccggcgcgc gtcgaggtgc
     2341 ccgcgtgggt ctacgaggcg gggctgctcg acgaagtgct cgacgccgtg cgcgccgagt
     2401 gcgtcgtcgg caacggctac ccctacgccg tcgagacggc ggacgaggcg gcggtgatga
     2461 cggcgcgcga ccgccagcag ttcctccgcg tcatgcagga cttcgccggc cggcacagct
     2521 tcgccttccg cgtctcgcgc aaggccgcca gcaaggcgcg ccgccgctga cgggggcgac
     2581 gcggggcgaa aaatatgtaa tcatcggcgc gagggattcc ggtcggtctc ggcgggtgtt
     2641 cgttatgatg agcgagtcga attttgacgc cggcttcgag ggacgcggcg aggacttcgt
     2701 ctcccgtcgc gggcaggagg cgtgtcccgc gtgcggcgcg caggcgcggc ggacggacgc
     2761 gcgcttctgc gcgacgtgcg ggcggcggct cgcggacgag gagtacttcc cgaccgacgc
     2821 gctgctcgcc tcgtaccacc agcagcggcg gcggccctcg ctcgacggag cggcggcgaa
     2881 gaaagtcggc gaccgcccgc gcgccgtctt ttacaagagg gacgcgcgcc cgcgcgcggc
     2941 gagacccgat atgttttccg acaggcacgg aaacaacgcg gcgcagacgg cgatggcctt
     3001 cgtggtctac gcgctggtgc cctacctcgg catcctcttc tgccccggcg cgctcttgat
     3061 gggcggcgtc ggtctgctgc gcgcgcgccg cgccccgcac gtcggcgggc accgcgcctc
     3121 tctggcgagc atcgcgctcg gcctcctgct cttcgtcgcg cacctcctgc tctggtggat
     3181 tctcttcaag gtgcccgagt ggacgcggca agccttctaa caggggccgg gtgtcggggg
     3241 tcgggctttt agttgactcg catcgaatct tgacgcacga ctcgccactt caccttaatg
     3301 aaaggtgttc accggccccc ggcctccgac acccggcccc tgcttttcta tgacgcgcat
     3361 cgccaagatc gtcaagtcga actcgcacgt cgattacgtg gggcgggtga tcgaccgcct
     3421 cgacgcggcg gagccgccga cgggcgagga ctacggcttc gcgcagttcg tggccgtccc
     3481 cgtcgccgaa ggcgtggagg tgatcggcgt cgtctacgac acgcagctcg ccaacccaga
     3541 gtacggcagc tacgggccgc ggctctcttc gcgcaccgag ctgtcggtct tgagtccgga
     3601 ctacctgaac gagcagggcg tgctcctcgg catcctgctg gtcggctggc gcgagcggcg
     3661 cggcgcgggc ggcgacggcg gcgagtggga aggccggcac gaagtcccgc gccgcgtcat
     3721 cccggtcggg caggacgtgt acgcgctcgg cgaccgggag gtgcaggact tccaccgcgg
     3781 cgagggcggc gccgtgcagc tccgctacta ctcgcaggtc atcacgcacg cgggcgcctt
     3841 cgccgtcccg ctcgtcgagg ccgtcatcgc gcagctcgaa cccgcgtgcg cgcccgccga
     3901 gcgccagcgc ctgtgcgtcc tgaaaaaatc actttcctgg caacgcacgc tcggcggcgt
     3961 gcgtctctaa caagacagtg atgaggactg aggactgagg actgagtaaa gacaaaccgc
     4021 tttcactcag tcctcagtcc tcagtacttc ctcagtagta agctttccgg agggaactca
     4081 tgaagaacac gcgcagactg ctggcggcgc tcgtcgccgg aattttcctc gccgccgcgg
     4141 gcgcgcaggc gcagcgcacg cgccccgtct cggccccggc gcccgagaag accgaggcga
     4201 agccgcccgt gccgggcgtg acggcgaacg tcccgcccgc cccgcagacg gtgaaggtga
     4261 agtacgaggg cggcgtcttc ggctacacca agaagatgga cggcacgctc accttcgacg
     4321 accagaaccg ccgcctcgtc ttccgcgacc agaagacgca gcaggagaaa atctccatca
     4381 actacgacgc catcgcctcg gccttcgccg acacgcagtc gaagcggccc atcgcggcgg
     4441 acgtgctggg ccgcgcctcg atcttcacgc tgccggcgct gctcatcaag aagaagttcc
     4501 gctacctcac gctccagttc aacgacgagc actcgaacgc gaccggcatc acgagcttca
     4561 agctggagaa caaggaaatc ctcgcctccg tcctcaacac gctcgcgcag aaggccggca
     4621 tgacccagcg cggcgaaatc ttcgtccgca acaaatccgc cgccgcgaag accgacgccg
     4681 gcacgccgaa ctgaaccggc gcacgaaaca gtatggaagg aaggggcggc gaagagagcc
     4741 ttcgccgccc cttacctttc acgtgcgcgc ctcaggcggt cgccttccag tcgtagctgt
     4801 cggccttgac ccgcaggtag ttttcgtcga gccgcgtcgt gagcaggagg acggcggcga
     4861 tgcgacgggc ggtctgtgtg acttcgcgcg cctcttccgg ggtgagcgag cggcccagta
     4921 tgtcgtgctc gcggtaggag agccactttt tgattacctg gtagccgcct acggtgtaat
     4981 cccacacgag cgccgggacg ttcgaccagt aggcggcgtc gttcaggaag acgtcgtacg
     5041 tctcggcgcc gagcgcgtgc gccacggtcg gcggagcctc gcgctccggc gcggcgtagt
     5101 cacgcgtgcg cgcgcggcct ttgccgggca tgacgacgcc ggccttgcct ctgtgccccc
     5161 agcccgcgcg cagctccaag tcaccggccc gcgggtcgag cgcgtgcgcg ccgtccgcgc
     5221 ggcgcagcac ggcgattgcg cgcagctcgg ggcgaatcgc gccgccggtc acgcccggca
     5281 cgggcgtctc ggtgtcgagg agccgcgcga cctgccgccc gagcgcggct gaactctcca
     5341 acagcccgcg cgcggcgggc agagggacgc gtgggaagtc cgcgcggatg ccgtcggcgt
     5401 tctccgcgag gtaagcgtgt gcgtggccga cggcgagcgc gtgataccag acgagcgcgg
     5461 cggcctccgc atcgccgtcc acctcgccga cgccgagcgc ggcgaggtag gcgcgggcgc
     5521 gcggcgagag gttggcggtt gtctctttct ccgccgtgcc tgcatggtcg agcaacgagc
     5581 tttgcttgtc gtcctttcga gacatctttc ctgtcgaggt acacaggcgg atcgggattg
     5641 cgaaaggatt taccgcgatg gcttgcccat cgaccaaagt ggtcgagaaa tgaatcgggc
     5701 ttccttcggg gttcttttcg cttttgagcc tgctgataat gaaaccgttt tttatccgat
     5761 tttgcgccca caggatcggg cgcggctcgt tccatagtgg gcgagtcggg ctgtaataag
     5821 cccatcgtgt atcgaaaggt ctgaccgcat agcgtcggag gcgactcggt tcgtacgtct
     5881 cagccgacgt gactttaacc cgcgccatct tcgcatcgaa tcgcgccgcg tccctggtca
     5941 gtcccgtatt caagcccttg agcgtttccc aatcgacgga cttatcgaag tacattccca
     6001 cgcgtttttc gagcgcgccg cggtcgatgt cgatcaacgc tccgccgcgc ttctcgaaaa
     6061 agccgtcgct tctcacttcg gacaattcgg tcagcttcgg ccacgcgaga tagtgcgcgc
     6121 cgaccgccga cgggcggaac gaatagcggt tcgtcgcgtc cggctgcgcg ggcgcgtagt
     6181 gcgcgtcgaa gtcttcggcg tcgaggccgg cgaacaagtc ttctcgcttc gtcacgcccc
     6241 acaactcgcg cgtgcggact tgcggctgtg cggcgcgttc ctctccgcgc gccagcaggc
     6301 cgatggccgt gccgacgcgg atgccttcgc ggttgtaccg cgtcgagaac acgctcgggt
     6361 cgggcttgcc ttcgggcgtg agcttgcccg tctcgcggct gtcgccgttc agcgagtcga
     6421 accacagctt gtcgaactcg ctcaggaacc tctgccgcat gacgacgaac gacgggtcac
     6481 tcaggtagga aaagttcgag atgaaacaga ctatcccgcg ccccgtctgc tccgcgatgc
     6541 gtcgctccgc gagccggaag aagcgcacgt acaaatcatc gagattgaat ttcttgatgc
     6601 cccattcctt gctcaagcct tgcttgtaaa cgtcaaccaa gtcgccttcc tcttccgcgc
     6661 tcgtcccggc gaaggcgttg tacggcgggt tgccgatgac gacgaggatc ggcgtctcgc
     6721 gtttgacgtg ctcggcggcg gcgcgctcct cttcgagcgc gtggaaggcg aagggcagtt
     6781 gctttttcgg gtcgcggggc ggctcccagc ccgtcagcgc gttcgtcagg tagacgccga
     6841 cgcgctcgcc ctcggcgagc gggctgccct ccgtctgcaa cagcaggccc aactgcaagt
     6901 gcgcgacgac gaacggcgcg ggcagaatct cgaagccgaa gacgcgcccg agcgccgccc
     6961 gcttcaggtc gttggccgcc ggcgcgtcgc cgccctgctc gtctatcgtc cgcttgatgc
     7021 gccgcaggac ttcgaccaga tacgcgcccg tgccgcaaca ggggtcgagc acgtagaccc
     7081 gctcatcggc cagcccgttt tcgatccgca gctcttcgcg caaggcccga tcggtgcgct
     7141 cgaccatgta gcgcacgatc tctccgggcg tgtaccagac cccgaggtgc ttccgcaatt
     7201 ccgggtcgaa ggcctcgagg aacggctcgt agaaatactg gacggcgtgc tcgcgctcga
     7261 accgctcgaa aaacttctcc gtctcgacgc ggttcaaagt gtccgccgtc cgatcgagaa
     7321 cctcttccag ccccagcccc cgcacgttcg acggcgtgac gacctgctcg aagagcgtgc
     7381 gaatcgcggg cacgtgcagc gtccacgtcg cgtcgtgcca gtggaattca tcccgccgcc
     7441 gcgcgccccg gtcggacttc gaccacagca cccacgcgga gaagacgccg taaaagagcg
     7501 tctgcacgag cgtcgagcgg aaaaactgct cgccctgcgc gccctcgaac ttcaggccga
     7561 gcgcctgctc caacgcctcg cgcacgttct tcaaagactg tgaggcgttc ggctcgcacc
     7621 ccacgcgctc tttcgcgtcg cgcgcgtaag aggccaggaa ccgcgcgagc gcttcgggcg
     7681 cgtccagcgg caccgaagac agcatgtagt tttcgaccct ggacatcgag caggtctccg
     7741 accgcggtag gttgtcggct gcgatgagta cgaaaagaag cggcgccggt gaagtcttcg
     7801 cgccgcctct ccctgacacg aaaagcagac cgggccgtca gtgctcgcgc cgcgcgcccg
     7861 cgggcggttc gccctggtaa agctcggcgc ccgcggcgac gaactcggcg gccttctcct
     7921 tcagccccgc gccgagggcc gactgttcgt ccaacgcctt cttcgcggcg tagtcgcgca
     7981 cctcctgcgt gatcttcatc gagcagaagt gcgggccgca catcgagcag aagtgcgcga
     8041 gcttggcgcc ctcctgcggc agcgtctcgt cgtggtactc gcgcgccacc tcggggtcga
     8101 gcgagaggtt gaactgatcc tcccagcgga actcgaagcg cgccttcgag agcgcgttgt
     8161 cacggagctg cgcgccggga tgccctttgg cgaggtcggc ggcgtgggcg gcgatcttgt
     8221 aggcgatgac gccgtccttc acgtcctgct tgttcggcag acccagatgc tctttcggcg
     8281 tgacgtagca gagcatggcg gtgccgaacc agccgatcat cgccgcgccg atggccgaag
     8341 tgatgtggtc gtagccgggc gcgatgtccg tggcgagcgg gccgagcgtg tagaacggcg
     8401 cctcttggca ggtttcaagc tgcctgtcca cgttctcttt gacgaggtgc a
//
LOCUS       DBNGOH010001181         2765 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2195663, whole genome shotgun sequence.
ACCESSION   DBNGOH010001181 DBNGOH010000000
VERSION     DBNGOH010001181.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2765)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2765)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2765
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2195663"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(419..790)
                     /locus_tag="AC159P_22860"
     CDS             complement(419..790)
                     /locus_tag="AC159P_22860"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3698342.1"
                     /translation="MPQMALTILYVEDDRLISAAVAELLTCEGWRVETCADGLAALAL
                     LEGDARYDLIMLDNELPHVNGVELARRARATAHRAQAPIIMLSASEVDAEARAAGVDL
                     FLRKPQDMGLIASAVESLVGR"
     gene            complement(942..1397)
                     /locus_tag="AC159P_22865"
     CDS             complement(942..1397)
                     /locus_tag="AC159P_22865"
                     /inference="COORDINATES: protein motif:HMM:NF013777.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HIR3698343.1"
                     /translation="MRRREGDGCPVWGALQVVGDKWTMLIVRDLVAGARRTTELLAGL
                     QPISSRTLMDRLRDMEHDRLIERRDFGGSPPHVEYTLTERGRLLAPLLDALRALGDAL
                     GCNDCDDRRARLDAYCDACPRAERPAAANNNPSHDAPRRAQRDDSIVLL"
     gene            1686..2660
                     /locus_tag="AC159P_22870"
     CDS             1686..2660
                     /locus_tag="AC159P_22870"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HIR3698344.1"
                     /translation="MRPEPTPARDAAKQNGQAPVILVADDEPVNLALIKRRLEWEDYH
                     IHTAQDGSEAVEKARLLRPDLVILDVMMPVLDGLQACRLLKDHEATRDIPIIFLSALD
                     DTDTKINGLSLGANDYISKPFRVEELLARVAVAIRLKRERDELRRSAEESQKRAEAAS
                     ERSMSDALTGLLNRYGLQRSLQRELSESRRYGRALSCLLIDIDFFKAVNDNYGHAAGD
                     TALMQVGRVLTEAVRGSDVVCRYGGEEFLVLAPETTLDGAMALADKIRLAAAARLFGD
                     GERVFPLTLSVGAAQLAQSESGHDMIARADVALYNAKEAGRNRVEAAR"
BASE COUNT          439 a          973 c          922 g          431 t
ORIGIN      
        1 tcccgaaagg tcttgccgcg ttagttgacg ccgcgcggcc tgaagtttcg tagaaatatt
       61 cggaggtcgg ctataagaac tgtcgcgccc cgccggggct tcccgcttcg tgcagttatc
      121 agaacgaacg tggccccgcg cgggttcgcc ttaaatgggg agcttcgggc gggcggctgt
      181 cccgaagtcc cgcgccacga cgaattcaca tcccgccccg tggtttttcg cgccgcgacg
      241 ctcagcaact aacctgcttc ggtcgcctgt cacgtcgcgg taataaggtt ttcacacgtc
      301 aagtacaacg cccgcagacg acggggccgc gcgcgtgccc gtcgcacgcg cgcggccccg
      361 cggcacagcc cgttcccttc agacgggcgt gcggcggtaa cgcctgcggc ccactgcgct
      421 accgccctac cagcgactcg acggcactcg cgatcagccc catgtcctgc ggctttcgca
      481 gaaacaggtc aacgcccgcc gcgcgcgctt ccgcgtccac ttcgctcgcc gagagcatga
      541 tgatcggcgc ctgcgcgcgg tgggccgtcg cgcgcgcgcg ccgcgccagc tcgacgccgt
      601 tcacgtgggg cagttcgttg tcgagcatga tgaggtcgta gcgcgcgtcg ccttcgagca
      661 gcgccagggc ggcgaggcca tcggcgcaag tctcgacgcg ccagccctca caggtgagca
      721 gttccgccac ggcggcggag atcagtcggt cgtcctcgac gtaaaggatg gtgagggcca
      781 tctgtggcat gttccgcgcg ctcccgccgg gcgacatggc ctcgcgaagt acggagtaat
      841 ggaacgggtc ttaccgtccc acggcaaaaa gagttgatcg aggtggcgga acgcggtcag
      901 aaaaatcccg gcatcgtgcg gtcggcaccg ccgttacttc cctagagcag gacgatggag
      961 tcgtcgcgtt gcgcgcggcg gggcgcgtcg tgcgaggggt tgttgttggc ggcggcgggt
     1021 cgctcggcgc gcgggcaggc gtcgcagtag gcgtcgaggc gcgcgcggcg gtcgtcgcag
     1081 tcgttacagc cgagcgcgtc gccgagcgcg cgcagggcgt cgaggagcgg cgcgagcaga
     1141 cgcccgcgct ccgtaagcgt gtactcgacg tgcggcgggc tgccgccgaa gtcgcggcgc
     1201 tcgatgaggc ggtcgtgttc catgtcgcgg aggcgatcca tcagcgtgcg gctgctgatg
     1261 ggttgcagcc ccgcgagcag ctcggtcgtg cggcgcgcgc ccgccaccag atcgcgcacg
     1321 atgagcatcg tccacttgtc gccgaccact tgcagcgcgc cccacaccgg gcagccgtcg
     1381 ccttcgcgtc gtcgcatgag tagagaccgt cctttccttc cagcccgaag cggctgccgc
     1441 ggccccggca gggcgccgga aactctgtgg gcgcgggcgc cttcggtgtg ggccgcgaaa
     1501 tcggcgcaga gcttagcaca aagacggggc tttgcggcgg cgataaattg ttttaagatg
     1561 agactcgcgc gaccccctcg ccagggcagt tccgttcccc ccgtccgtag gccccaacct
     1621 cacgggctgc tgccgcatcc aaaattcagc tcacgggcaa tttgtcccct gcgccggaca
     1681 ccgttatgag gccagaaccg acacccgcgc gggacgccgc gaagcagaac ggccaggccc
     1741 cggtcatcct cgtcgccgac gacgagccgg tcaacctcgc gctcatcaaa cgccgcctgg
     1801 agtgggagga ctaccacatc cacacggcgc aggacggcag cgaggcggtc gagaaggcgc
     1861 ggctgctccg ccccgacctc gtcatcctcg acgtgatgat gcccgtcctc gacggcctcc
     1921 aggcgtgccg cctcctgaaa gaccacgagg cgacgcgcga catcccgatc atcttcctct
     1981 cggcgctcga cgacaccgac accaagatca acggcctctc gctgggcgcg aacgactaca
     2041 tctcgaagcc cttccgcgtc gaggagctgc tggcgcgcgt ggcggtcgcc atccgcctca
     2101 agcgcgagcg cgacgagctg cgccgcagcg ccgaggagtc gcaaaagcgc gccgaggccg
     2161 ccagcgagcg ctcgatgtcg gacgcgctga cgggcctcct gaaccgctac ggcctccagc
     2221 gctcgttgca gcgcgagctg tcggagtcgc gccgctacgg gcgcgcgctc tcgtgcctgc
     2281 tgatcgacat cgacttcttc aaggcggtca acgacaacta cggccacgcc gcgggcgaca
     2341 ccgcgctgat gcaggtcggg cgcgtgctga ccgaggccgt gcgcggctcg gacgtggtgt
     2401 gccgctacgg cggcgaggag ttcctcgtgc tcgcgcccga gacgacgctc gacggcgcga
     2461 tggcgctcgc cgacaagatt cgcctcgccg ccgccgcgcg cctcttcggc gacggcgagc
     2521 gcgtcttccc gctcacgctc tcggtcggcg ccgcccaact cgcacaatcc gaatccggcc
     2581 acgacatgat cgcacgcgcc gacgtggccc tctacaacgc caaagaggcg ggccgcaacc
     2641 gcgtcgaagc cgcgagatag agagaagggg gcgaagggga gacaaggagt gggggagatt
     2701 agccgcacgg cttttatctc cccactccct ttctcccttt ctccccttct ccctttcgcc
     2761 tttac
//
LOCUS       DBNGOH010001182         2464 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2196065, whole genome shotgun sequence.
ACCESSION   DBNGOH010001182 DBNGOH010000000
VERSION     DBNGOH010001182.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2464)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2464)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2464
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2196065"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(53..520)
                     /locus_tag="AC159P_22875"
     CDS             complement(53..520)
                     /locus_tag="AC159P_22875"
                     /inference="COORDINATES: protein motif:HMM:NF025719.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsbRD N-terminal domain-containing protein"
                     /protein_id="HIR3698345.1"
                     /translation="MPEEISRLLQSGQETIVRNWTEKVTADRRINSDERLSYLQLVDH
                     IPQIVEELRLALLYEQRGGAMLTQGAEHGRQRWQQGYELKEVVRELTLLRATLMEFLD
                     TYSGALGTRTFDNLSRSYQKINWFMDDEIYRTVEAYLEAHRGQPPGHGTHEGN"
     gene            744..959
                     /locus_tag="AC159P_22880"
     CDS             744..959
                     /locus_tag="AC159P_22880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3698346.1"
                     /translation="MEINKDSIVTQEETGEPAPVDEVEGRVEAEMKRIEGSARESVAQ
                     GMQDKEREREGRELKEEGERELDEQKK"
     gene            complement(1129..1815)
                     /locus_tag="AC159P_22885"
     CDS             complement(1129..1815)
                     /locus_tag="AC159P_22885"
                     /inference="COORDINATES: protein motif:HMM:NF020123.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HIR3698347.1"
                     /translation="MNLIRQTQSSLRGATRGALAAVVAAATLFAAGCTPPEATSNGAG
                     NNANAVAANGAKKPSVTITQGTPARQAATPAPALETVPPDVWNAEIQALDGKTFRLSD
                     FKDKTIVLDIWATWCGPCRLDVPHLVEMTDDYKNKGVQVIGLTTENPEADEEKVRDFV
                     KEFKINYKIGWATASIALPLMQGSASIPQTFVIAPGGKILVKYRGYSAQKPAAIRAMI
                     DKSQDAKTGD"
     gene            complement(1982..>2464)
                     /locus_tag="AC159P_22890"
     CDS             complement(1982..>2464)
                     /locus_tag="AC159P_22890"
                     /inference="COORDINATES: protein motif:HMM:NF015722.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HIR3698348.1"
                     /translation="VNNVSYEDAQKFAAWRSGRDKTTYRLPTEEEWEYAARGGGDSVR
                     AYPWGDEWAEGRANLNAEAPQPVGSFPQGRTPQGADDMLGNVWEWTSSTAAMYKGNSR
                     TQLEAKDRGKLVVRGGSYESTHTGNEPVTVTSRRWLARDFRSPVLGFRLVREGGSGER
                     "
BASE COUNT          399 a          745 c          832 g          488 t
ORIGIN      
        1 gagaagaact cacttctctt gtcacggttc acggttcacg gtttgcggct tttcagttgc
       61 cttcgtgcgt gccgtggccg gggggctggc cgcggtgggc ttcgaggtag gcttcgacgg
      121 tgcggtagat ttcgtcgtcc atgaaccagt tgatcttctg gtaggagcgc gagaggttgt
      181 cgaaggtgcg cgtgccgagc gcgccgctgt aggtgtcgag gaactccatg agcgtggcgc
      241 gcagcagcgt cagctcgcgg acgacctcct tcagctcgta gccctgctgc cagcgctgcc
      301 gcccgtgctc ggcgccctgc gtgagcatcg cgccgccgcg ctgctcgtag aggagcgcga
      361 gccgcagctc ttcgacgatc tgcgggatgt gatcgacgag ttgaaggtag gacaggcgct
      421 cgtccgagtt gatgcgccgg tcggcggtga ctttctccgt ccagttgcgg acgatggtct
      481 cctggccgct ctgcaatagg cgactgatct cttctggcat cgggggaacc ttccggctgt
      541 cttaaagtgg tggcagaggc ggcgatcaag cacggggagt gctcggtcgc cgcaactcac
      601 tcaagcgggc ggattatacg ccgcgaggcg cgcggcgggc aacagagcgg cgggcgacgg
      661 atttgaccgc ggggggccga cggtgcgacg attccccatc ctttgttttc gggaaatcca
      721 gattcggggg ggggtgacgc gggatggaga ttaacaagga cagtatcgtg actcaggaag
      781 agacgggcga gccggcgccg gtggacgagg tcgaggggcg cgtcgaggcc gagatgaagc
      841 gcatcgaagg cagcgcacgc gagtcggtcg cgcaaggcat gcaggacaag gagcgcgagc
      901 gcgaagggcg cgagctgaaa gaagagggcg agcgcgaact cgacgaacag aagaagtagg
      961 cagtaggcag taaaagaaag acacgtaaag acgcttgaga tggaatgtct caagcgtctt
     1021 cactttaccg cttatcgaac gaagaggctt gggatatttc atcccaagcc tctttccttc
     1081 ttctgttttg actgcctacc gcccgctgcc gactgcctac tgctcccttc agtcccccgt
     1141 cttggcatcc tgcgacttgt cgatcatggc gcggatggcg gcgggcttct gcgccgagta
     1201 gccgcggtat ttgacgagaa tcttgccgcc gggcgcgatg acgaaggtct gcgggatgct
     1261 ggcgctgccc tgcatgagcg ggagcgcgat ggaggcggtc gcccagccga ttttgtagtt
     1321 gatcttgaac tccttgacga agtcgcgcac cttctcctcg tccgcctcgg ggttctcggt
     1381 cgtgaggccg atcacctgca cgcccttgtt cttgtagtcg tcggtcatct cgaccagatg
     1441 cggcacgtcg agccggcacg gcccgcacca cgtcgcccag atgtcgagga cgatggtttt
     1501 gtctttgaag tcggagaggc ggaacgtctt gccgtcgagc gcctgaatct ccgcgttcca
     1561 cacatcgggc gggactgttt cgagcgcggg cgcgggcgtc gccgcctgcc gcgccggggt
     1621 gccctgcgtg atcgtcacgc tcggcttctt ggccccgttc gccgccaccg cgttcgcgtt
     1681 gttgcccgcc ccgttgctcg tcgcctccgg cggcgtgcag cccgcggcga acagcgtagc
     1741 ggcggcgacg accgcggcca gcgcgcctcg cgtcgcgccg cggagacttg attgcgtctg
     1801 tcgaattagg ttcattactt cgagattcct tttgatgatg cgacgaatat aaacacaaag
     1861 acactcggat tgtagatttc ggatttcgga tttcggatta acagaggcgt tgacgattgc
     1921 tgacagcacc gctcccatca atcgttaatc gttaatccga aatctacaat ccgaaatctc
     1981 ctcaccgctc accgctgccg ccttcgcgga cgaggcggaa gccgagcaca ggggagcgga
     2041 agtcgcgcgc gagccagcgg cgcgacgtga cggtgacggg ttcgttgccg gtgtgcgtgc
     2101 tctcgtagga gccgccgcgg acgacgagct tgccgcggtc tttcgcttcg agctgcgtcc
     2161 gggagttacc tttgtacatc gcggcggtcg aactcgtcca ctcccacacg ttgccgagca
     2221 tgtcgtcggc gccctgcggg gtgcgcccct gcgggaacga gccgaccggc tgcggcgcct
     2281 ccgcgttcag gttcgcgcgc ccttcggccc actcgtcgcc ccacgggtag gcgcgcacag
     2341 agtcgccgcc gccgcgcgcc gcgtactccc actcctcttc ggtcggcagg cggtacgtcg
     2401 tcttgtcgcg cccggagcgc cacgcggcga acttctgcgc gtcttcgtag gagacgttgt
     2461 tgac
//
LOCUS       DBNGOH010001183         7218 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2196278, whole genome shotgun sequence.
ACCESSION   DBNGOH010001183 DBNGOH010000000
VERSION     DBNGOH010001183.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 7218)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7218)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7218
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2196278"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..962
                     /locus_tag="AC159P_22895"
     CDS             <1..962
                     /locus_tag="AC159P_22895"
                     /inference="COORDINATES: protein motif:HMM:NF023945.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HIR3698349.1"
                     /translation="VFVSEEAVQSAEKLIRAVFGGPRDAIEGEAPAPEVLTGRLENAL
                     GYRKDAWRTETIRRLCDVLAEVASGRKKGRNYEARWLNLYGFCLRPGFGAALDEWRVT
                     QSRKVYHDGLAFPKDLQCQVEWLILWRRVAGGMGAGQQRELYERHRAQLGVGGAKAKG
                     GRINRQVEQEGWLLLASLEHLPATLRAKLGDELLARVEEEPDNKSLLWALGRLGARFP
                     FYGPLNCVAPAESAARWATALLRLPALTTHAASAVAQLAARTDDPLRDVEPDIRHVAI
                     ERLTAAGADEQLVESLRTCQPPTRTDAARIFGESLPEGLRLLS"
     gene            complement(1127..3241)
                     /locus_tag="AC159P_22900"
     CDS             complement(1127..3241)
                     /locus_tag="AC159P_22900"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004324477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HIR3698350.1"
                     /translation="MKPKRSVLLALVLILSACVPASAQSPLPSSPPSKFFREPDMMTI
                     GVYYYPEAWDKAQWARDISNIKKLGMEFIHMGEFAWAFMEPKEGEFDLDWLAENVELA
                     TRQGLKVILCTPSAAPPVWLTKKHPEILMVDAAGRRQNHGARQHACWSAETYRKYVER
                     VNTQLARRFGDNPNVIGWQIDNELTHYGKPYCYCEHCQAKFRKWLKAKYGDVKNLNRD
                     WGNAFWSQMYQSFEQIDIPNAQQFVALPNPHAVLDHQRWFAEEAADYLRFQADTLRRH
                     AKNQWVTTNFINRYPPVSPPLSGRDLDIITWSLYPVAGLYPDAEKFRFGDGAAMSWMH
                     DYARTINGAQGVMELQPGQVNWGPVNPQPYPGAVRMWIMRAFAAGSKLVCTYRYRQPL
                     YGNEQYHYGIVGTDGVTPSTGGRQFEQAMREIRTLRALYRPNAKEPARYAARRTAFLY
                     NVENQWNLDNHTQTTRWDTWGHLFKYYRALKGFGAPVDVVTEDKDFAEYPFLVAPAYQ
                     LLDEKLVRRWTEYVERGGHLVLTLRTGQKDRRGHLWEASYAAPISELIGAGVESYDVL
                     PDPLKGKARAGAKVYEWAVWGESLKPKAGTTALATYADQFYAANVAAVTRRLGRGTVT
                     YVGVESLTGELERDLLRRVFTTAGVAVENYEDQFLVDWRDGFWVATNFTSKRQLAPAP
                     PNAKILIGTRTVEPAGVTVWME"
     gene            3377..4084
                     /locus_tag="AC159P_22905"
     CDS             3377..4084
                     /locus_tag="AC159P_22905"
                     /inference="COORDINATES: protein motif:HMM:NF013858.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreF"
                     /protein_id="HIR3698351.1"
                     /translation="MSDEALRRLWLMQLSDSALPVGALSHSFGVETLAAEGALSVETL
                     EGFLRDYLGEVGLLESAFCRAAHRLGRRDAGAFAVEEWLRLNARLGALKPARESRTGG
                     AVLGRRFLRLAAELCAHGRMAEALEVAERTAVEIQHCAAFGLAGGALGIDEDATALAY
                     LHQTLANLVSACQRLLPLGQQRASGILWRLKPALVEACEQSRAAAYDLDEAACFAPLL
                     ELASMRHPGLETRLFIS"
     gene            4084..4980
                     /locus_tag="AC159P_22910"
     CDS             4084..4980
                     /locus_tag="AC159P_22910"
                     /inference="COORDINATES: protein motif:HMM:NF013899.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HIR3698352.1"
                     /translation="MHTALPVDAPLKVDAPRVHGRLRLRFETERRTRATVLRVCEQRP
                     PLKVVRAFPTGDGGALVHLHNLSGGVLGDDLLELSVEVGPAAIAQLTSTGATRLYRSR
                     DGARGARQLTRVSVGTGGLLEYLPDPLIPFAGSRYRQETTVELAAGAGLFWWETVAPG
                     REARGELFAYERLRLKTDITSEGRPIALERIDLEPARRPLDSPLRLGPYRYLSSFHVC
                     RTGVEPRRWLELEATLSELARRLSAAGRVLWGVSALRAHGLLVRALGVKGWDIQTGLP
                     DFWQAAKSYLYGRAATPPRKIY"
     gene            5175..5477
                     /gene="ureA"
                     /locus_tag="AC159P_22915"
     CDS             5175..5477
                     /gene="ureA"
                     /locus_tag="AC159P_22915"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015935616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit gamma"
                     /protein_id="HIR3698353.1"
                     /translation="MHLTPREQEKLLIFVAAELARKRRARGLRLNYPEAVAVLSAEIM
                     EAARDGKTVAEIMTFGASILTSEDVLEGVAAMIHEVQVEATFPDGTKLVTVHDPVR"
     gene            5481..5843
                     /locus_tag="AC159P_22920"
     CDS             5481..5843
                     /locus_tag="AC159P_22920"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013809825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0035550 - urease complex [Evidence IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit beta"
                     /protein_id="HIR3698354.1"
                     /translation="MRPGEYFLDTEAGDIEANAGRRTAVVSVENTGDRPVQVGSHYHF
                     FEVNRALRFERAAAYGMRLQTAAGTAVRFEPGESKEVTLVEFGGARKVFGHRALVNGA
                     LDDDETKAAALRKEHEGA"
     gene            5868..6665
                     /locus_tag="AC159P_22925"
     CDS             5868..6665
                     /locus_tag="AC159P_22925"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017730474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HIR3698355.1"
                     /translation="MRYLSALCLLLPLSVAAHAKGPVTIYLAGDSTMAEKRPDKRPET
                     GWGESLQRFFDPSKVRVENHAQNGRSTKTFIAEGRWRALVEKLKPGDYVFIQFGHNDQ
                     PKEKASHTPPADYRANLVRFAGEVRAKKAAAVLFTPVVRRRFDDKGEFYDTHFEYPDI
                     VRAVAAEQRVALIDMHRKSERLLRQYGVEESKKLFLQLKPGEHPNYPKGVEDNTHFSP
                     RGAEEMARLAVAGVRELKLGLAKHPKKKSEVGSRNENRQPLLFYSGF"
     gene            6685..>7218
                     /locus_tag="AC159P_22930"
     CDS             6685..>7218
                     /locus_tag="AC159P_22930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012189993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR3698356.1"
                     /translation="MTLRIPRRTYADLYGPTVGDRVRLADTELLVEVERDFTVYGDEV
                     AFGGGKVIRDGMGQSSGATREGPAPALDLVITNALVIDHWGVVKGDIGVRGGRIVKVG
                     KAGNPDTMEGVDPELVIGAATEVVAGENRIVTAGAVDSHVHFISPQQIYAALAGGTTT
                     MIGGGTGPATGTNATTCT"
BASE COUNT         1171 a         2398 c         2464 g         1185 t
ORIGIN      
        1 aggtcttcgt ctcggaagag gcggtgcagt cggcggaaaa gctgattcgc gcggtgttcg
       61 gcgggccgcg cgacgccatc gagggcgagg cgcccgcgcc cgaagtcctg accgggaggc
      121 ttgagaacgc gctcggctat cgcaaggacg cgtggcgcac ggagacgatt cgcaggctgt
      181 gcgacgtgct ggcggaagtc gcaagcggtc ggaagaaggg gcgcaactac gaggcgcgct
      241 ggctcaacct ctacggcttc tgtctcaggc cgggcttcgg cgcggcgctc gacgagtggc
      301 gcgtcacgca gtcgcgcaag gtctatcacg acggcctcgc gttcccgaaa gatttgcagt
      361 gtcaggtgga gtggctgatc ttgtggcggc gcgtggcggg cgggatgggc gcggggcagc
      421 agcgcgaact ctacgagcga caccgcgcgc agctcggcgt cggcggcgcg aaggcgaagg
      481 gcggcaggat caaccggcag gtcgagcagg agggctggct cctgctggcg agcctcgaac
      541 acctgcccgc cactctgcgc gccaaactcg gcgacgaact gctcgcacgc gtcgaagaag
      601 aacccgacaa caagagcctg ctctgggcgc tcgggcggct cggcgcgcgg ttccccttct
      661 acggcccgct caactgcgtc gcgcccgccg agtctgcggc gcgctgggcg accgccctgc
      721 tgcggctgcc cgcgctgacg acgcacgcgg cctccgccgt cgcccaactg gccgcgcgca
      781 cagacgaccc gctgcgcgac gttgaacccg acatccggca cgtggccatc gaaagactca
      841 ccgccgcggg cgccgacgaa cagctcgtcg aaagcctccg cacctgccag ccgcccacgc
      901 gcaccgacgc cgcgcgcatc ttcggcgagt cgctgcccga aggcttacgc cttctgagtt
      961 gagcgtggcg cacgttcggt tcaatgcaga gcgcgcagag aagaaagagc ggagttgaaa
     1021 gctcgaatcc gttggttgtc ttctctgcgt cctctgcgcc cctccacgcc ctctgcgtcg
     1081 aatccgaacc cgcggcgaat tcagctggac atcatcgtgt tgacgcttat tccatccaga
     1141 ccgtcacgcc cgcaggctct accgttctcg tgccgatcaa tatcttcgcg ttcggagggg
     1201 cgggcgcgag ttgtctcttc gaggtgaagt tggtggcgac ccagaagccg tcgcgccaat
     1261 cgacgaggaa ctggtcttcg tagttttcga cggcgacgcc cgccgtcgtg aagacgcggc
     1321 gcagcaggtc tctctcaagc tcgccggtca aggattcgac gccgacgtag gtgacggtgc
     1381 cgcgcccgag gcggcgcgtg acggcggcga cgttggcggc gtagaactgg tcggcgtaag
     1441 tcgccagcgc ggtcgtgccc gccttcggct tcaggctctc gccccagacg gcccactcgt
     1501 agaccttcgc gcccgcgcgc gccttgccct tcaaggggtc gggcagcacg tcgtacgact
     1561 cgacgcccgc gccgatcagc tcacttatcg gcgcggcgta cgacgcctcc cacaaatggc
     1621 cgcgacgatc cttctgcccc gtgcggagcg tgaggacgag atggccgcct ctctccacgt
     1681 actccgtcca gcggcgcacc agcttttcgt cgagcagttg ataagcgggg gcgacgagga
     1741 aggggtactc cgcgaagtcc ttgtcttcgg tgacgacatc gacgggcgcg ccgaagccct
     1801 tcaaagctct gtagtatttg aagaggtgac cccacgtgtc ccagcgcgtg gtctgcgtgt
     1861 ggttgtcgag gttccactgg ttctcgacgt tgtagaggaa ggccgtgcgc cgcgccgcgt
     1921 agcgcgccgg ctctttcgcg ttcgggcggt agagcgcgcg gagcgttctg atctcgcgca
     1981 tcgcctgctc gaactgccgc ccgcccgtgg aaggcgtcac gccgtccgtg ccgacgatgc
     2041 cgtagtgata ctgctcgttg ccgtagagcg gctggcgata acgatatgtg cagacgagct
     2101 tcgagccggc ggcgaaggcg cgcatgatcc acatccgcac cgcgccgggg tagggctgcg
     2161 ggttgaccgg cccccagttg acctgtccgg gctgtagctc catcacgccc tgcgcgccgt
     2221 tgatcgtccg cgcgtagtcg tgcatccacg acatcgccgc gccgtcgccg aagcggaact
     2281 tttcggcgtc cgggtaaagc ccggcgaccg ggtagagcga ccacgtgatg atgtcgaggt
     2341 cacggcctga gagcggcggc gagacgggcg ggtagcggtt gatgaagttg gtcgtcaccc
     2401 actggttctt cgcgtgcctg cgcagtgtgt ccgcctggaa tctcaagtag tcggcggcct
     2461 cttcggcaaa ccagcgctga tgatcgagga cggcgtgcgg gttcggcagc gcgacgaact
     2521 gctgcgcgtt cgggatgtcg atctgctcga agctctggta catctgcgac cagaaggcgt
     2581 tgccccagtc gcggttcagg ttcttcacgt cgccgtactt cgctttcagc catttgcgga
     2641 acttggcctg gcagtgttcg caataacagt agggcttgcc gtagtgcgtc agctcgttgt
     2701 cgatctgcca gccgatgacg ttcgggttgt cgccgaagcg tcgggcgagc tgcgtgttga
     2761 cgcgctcgac gtacttgcgg taggtctcgg cgctccaaca ggcgtgctgg cgcgcgccgt
     2821 ggttctgccg gcggcccgcg gcgtccacca tcagaatctc ggggtgcttc ttggtcagcc
     2881 agacgggcgg cgccgcgctc ggcgtgcaga ggataacctt caagccctgc cgcgtcgcca
     2941 gctcgacgtt ctcggccagc cagtcgaggt cgaactcgcc ctccttcggc tccatgaagg
     3001 cccacgcgaa ctcgcccatg tggatgaact ccatcccgag cttcttgatg ttcgagatgt
     3061 cgcgcgccca ctgtgccttg tcccacgcct ccgggtagta gtagacgccg atggtcatca
     3121 tgtccggctc gcggaagaac ttcgacggcg gcgaagacgg gagcggcgac tgggccgacg
     3181 cggggacaca cgccgacagg atcagtacga gcgcgaggag cacgctgcgc ttgggtttca
     3241 tggcaagacc ttccggctgt cgttggatgg gtggagctga acggaaggca tcttaccatc
     3301 attgacttct gttgttgcca gtcggatgcg agacgtgctg gcccgcgcgc ggcggactgt
     3361 gctagcgtcg tcggccatga gcgacgaggc gttgaggcgg ttgtggctga tgcagctctc
     3421 ggacagcgcg ctccccgtgg gggcgctgtc gcactcgttc ggcgtcgaga cgctcgcggc
     3481 ggaaggggcg ctctcggtcg agacgctcga aggtttcctg cgcgactacc tcggcgaggt
     3541 cgggctgctc gaatccgcct tctgtcgcgc cgcgcatcgc ttggggaggc gcgacgcggg
     3601 ggcgttcgcc gtcgaggagt ggctgaggct gaacgcgcgg ctcggcgctt tgaagcccgc
     3661 gcgcgagagc aggacgggcg gcgccgtgct ggggcgtcgc tttctgcggc tggccgccga
     3721 actctgcgcg cacggtcgca tggcggaagc gttggaggtg gccgaacgca cggcggtcga
     3781 gatacagcac tgcgcggcgt tcggcctcgc gggcggcgcg ctgggcatcg acgaggacgc
     3841 gacggcgctc gcgtacctgc accagacgct cgccaacctc gtctccgcgt gtcaacgcct
     3901 gctgccgctc ggccagcagc gcgcgagcgg gattctgtgg cgactcaagc ccgcgctcgt
     3961 cgaagcgtgc gagcagagtc gcgccgccgc ctacgacctc gacgaagcgg cgtgcttcgc
     4021 gccgctgttg gaactggcgt cgatgagaca ccccggcctt gagacgcggc tcttcatcag
     4081 ttgatgcaca ctgctttacc ggttgacgcg cctttaaagg ttgacgcgcc gcgcgtccac
     4141 gggcggctgc ggctgcggtt cgagaccgag cggcggacgc gggcgaccgt gctgcgtgtc
     4201 tgcgagcagc gcccgccgct caaggtcgtg cgcgccttcc cgaccggcga cggcggcgcg
     4261 ctcgtgcatc tgcacaacct ctcgggcggc gtcttagggg acgacctgct ggagctgtcc
     4321 gtcgaggtcg gcccggcggc catcgcgcag ttgacttcga cgggcgcgac gcgcctctac
     4381 cgcagccgcg acggcgcgcg cggcgcgcgt cagctcacgc gcgtcagcgt cggcacgggc
     4441 ggcctgctcg aatacctgcc cgacccgctc atccccttcg ccggttcgcg ttaccgacag
     4501 gagacgaccg tcgagctggc ggcgggcgcc ggcctcttct ggtgggaaac ggtcgcgccg
     4561 gggcgcgagg cgaggggcga gctgttcgcc tacgagcggc tgcgcctcaa gaccgacatc
     4621 acctcggaag gcagacccat cgcgcttgaa cgcatcgacc tcgaacccgc gcgaagacct
     4681 ttggactcgc ctctgcgact cggcccctac cgctaccttt cgagcttcca cgtctgccgc
     4741 acgggcgtcg agcctcggcg ctggctggaa ctcgaagcga ctttgagcga gctggcgcgt
     4801 cgcctctccg cggcgggccg cgtcctctgg ggcgtcagcg cgctccgcgc ccacggcctg
     4861 ctcgtccgcg ccctcggcgt caaaggctgg gacatccaga ccggcctccc cgacttctgg
     4921 caagccgcca agtcgtacct ctacgggcgg gccgcgaccc cgccaagaaa gatttattga
     4981 ttgcggattg cggattgcgg attggcgtgc tgcccatgct tgaagtccga tttcgggcgt
     5041 tcgactctcg gcgcacgaac aatccgcaat ccgcaatccg caatccctta atgggtctat
     5101 aaacctattc aatgacgggc gcgcggcgcg gtgtgttaaa ctgcgcggcc tctgcgtcga
     5161 aggagaaacg cgccttgcat ctgaccccgc gcgagcagga gaagctgttg atcttcgtgg
     5221 ccgccgagct ggcgcgcaag cggcgggcgc gcggcctcag gctcaactac ccggaggccg
     5281 tcgccgtcct ctcggcggag atcatggagg cggcgcgcga cggcaagacc gtggccgaga
     5341 tcatgacctt cggcgcgtcc atcctgacga gcgaagacgt gttggagggc gtcgccgcga
     5401 tgattcacga agtgcaggtc gaggccacgt tccccgacgg gacgaagctc gtcaccgttc
     5461 acgaccccgt gaggtagaag atgcgaccgg gcgaatactt cctcgacacg gaggcgggcg
     5521 acatcgaggc caacgccggg cggcgcacgg cggtcgtctc ggtcgagaac accggcgaca
     5581 ggcccgtgca ggtcggcagc cactaccact tcttcgaggt caaccgcgcc ctcaggttcg
     5641 agcgcgccgc cgcctacggc atgcgcttgc agacggccgc gggcaccgcc gtccgcttcg
     5701 agccgggcga gagcaaagag gtcacgctcg tcgagttcgg cggcgcgcgc aaagtcttcg
     5761 gccaccgcgc actcgtcaac ggggcgctcg acgacgacga aacgaaggcc gccgccctcc
     5821 gtaaggaaca cgaaggggcg tgataaggaa cacgaagagt cttgatgatg cgttacctgt
     5881 ccgcgctgtg cctgctgttg ccgctgtcgg tcgccgccca cgccaaaggg ccggtcacga
     5941 tctacctcgc gggcgattcg acgatggccg agaagcgccc cgacaagcgg cccgagacgg
     6001 gctggggcga gtcgctgcaa cggttcttcg acccgtcgaa agtccgcgtc gagaaccacg
     6061 cgcagaacgg caggagcacc aagaccttca tcgcggaggg gcgctggcgg gcgctcgtcg
     6121 agaagctgaa accgggcgac tacgtcttca tccagttcgg ccacaacgac cagccgaagg
     6181 agaaggcttc gcacacgccg cccgccgact accgcgcgaa cctcgtccgc ttcgcgggcg
     6241 aagtgcgcgc gaagaaggcc gccgccgtcc tcttcacgcc cgtcgtgcgc cgccgcttcg
     6301 acgataaggg cgagttctac gacacgcact tcgaataccc cgacatcgtc cgcgccgtcg
     6361 ccgcggagca aagggtcgcg ctcatcgaca tgcaccgcaa gagcgagcgc ctcttgcggc
     6421 agtacggcgt cgaagagtcg aaaaagctct tccttcaact caagcccggc gagcacccca
     6481 actacccgaa gggcgtcgaa gacaacacgc acttctcccc gcgcggcgcc gaagagatgg
     6541 cccgactcgc cgtcgcaggc gtccgcgaac tcaagctcgg tttagccaag cacccgaaaa
     6601 agaagtcgga agtcggaagt cggaatgaaa accggcaacc gcttctgttt tattctggct
     6661 tctgacttct gacttctatt ttttatgacc ctccgcatcc cgcgacgcac gtacgcagac
     6721 ctctacgggc cgaccgtggg cgaccgcgtg cggctcgccg acacggaact cttggtcgag
     6781 gtggagcggg acttcaccgt gtacggcgac gaggtggcgt tcggcggcgg caaggtcatc
     6841 cgcgacggca tggggcagag cagcggcgcg acgcgcgaag ggccggcgcc cgcgctcgat
     6901 ctggtcatca cgaacgcgct cgtcatcgac cactggggcg tcgtgaaggg cgacatcggc
     6961 gtgcgcgggg ggcgcatcgt gaaggtcggc aaggccggca acccggacac gatggagggc
     7021 gtcgatcccg aactcgtcat cggcgccgcg accgaagtcg tcgcgggcga gaacaggatc
     7081 gtcacggcag gcgcggtcga tagtcacgtc cacttcatct cgccgcagca gatttacgcg
     7141 gcgctcgcgg gcggcacgac gacgatgatc ggcgggggca ccggcccggc caccggcacc
     7201 aacgcgacca cctgcacg
//
LOCUS       DBNGOH010001184         1746 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4032014, whole genome shotgun sequence.
ACCESSION   DBNGOH010001184 DBNGOH010000000
VERSION     DBNGOH010001184.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 1746)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1746)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1746
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4032014"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            182..496
                     /locus_tag="AC159P_22935"
     CDS             182..496
                     /locus_tag="AC159P_22935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3698357.1"
                     /translation="MSTDRFTEIINLMSAMSREIGAFRAEVNARFDSLEARVSNLGTR
                     VGNLETEMRAGFEQVRADIRRLSHKIEELTQDSMELRADQRDLRKRTEVLEKKAGITD
                     SV"
     gene            499..>1746
                     /gene="nuoH"
                     /locus_tag="AC159P_22940"
     CDS             499..>1746
                     /gene="nuoH"
                     /locus_tag="AC159P_22940"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HIR3698358.1"
                     /translation="MLTLLAPPSFIDELLLRVFSPETVNYVVWPFIQIGAVVTAVAVW
                     AAYATYLERKISAFMQARLGPMRVGPWGLLQPIADAVKLLTKEDITPDKADNYIFKFA
                     PYISVVAAFVVFVVVPFGPAWGVITDINIGLLFILSVSSVGVLALILAGWSSNSKYAL
                     LGGLRSSAQMVSYEVSMGLSLVGALMFARTLSMSGIVGAQAADSVWFILYQPLGFVIF
                     LVSGIAENNRAPFDLPEAESELVAGFHTEYSGMRWSLFFMAEYAAMIVVSAVATAVYL
                     GGWYFPGVLWLAGGYNNVWVLLSLLVFLAKMALLLYLYFWMRWTVPRYRYDQLMDLGW
                     KWMIPASLINIVWTGLAVFAVQALDGWRGFDTVESLTRGLNLTATGKAVMIAFGLAGV
                     LLAAALLSAFNWRSRDFNLKKQRR"
BASE COUNT          279 a          552 c          568 g          347 t
ORIGIN      
        1 taccgcggca cctggcacac gccgcccttc gccaccggcg agtggtactc ctacctcgtc
       61 gttgaccgca gcggcacgct cgacgacgac tacgagctgg ccgacttgaa ggcgacgcgc
      121 gactgcgttt tcgagatcga tttgtgaatc ccgcccttca ccacgaagga gggaaacgac
      181 catgagcaca gaccgcttca ccgaaatcat caatctcatg agcgcgatga gccgcgagat
      241 cggcgccttc cgcgcagagg tcaacgcgcg cttcgacagc ctcgaagcac gcgtgagcaa
      301 tctcggaact cgcgtcggta atctcgaaac ggaaatgcga gccggtttcg agcaggtgcg
      361 cgcggacatt cgccgcttaa gccataagat agaggagctg acgcaggact caatggaact
      421 gcgcgccgac cagcgcgact tgaggaagag aacggaggtg ctcgaaaaga aagcgggcat
      481 cacggattcg gtatgagtat gctgacgttg cttgcgccgc cgagtttcat cgacgagctg
      541 ttgctgcggg tcttcagccc ggagacggtg aactacgtcg tctggccgtt catacagatc
      601 ggcgccgtgg tgacggcggt cgccgtgtgg gcggcctacg cgacctacct cgaacggaaa
      661 atctcggcct tcatgcaggc gcggctgggg ccgatgcggg tggggccgtg ggggctgttg
      721 cagccgatcg cggacgccgt caagctcctg accaaggaag acatcacgcc ggacaaggcg
      781 gacaactaca tcttcaagtt cgcgccgtac atctcggtgg tcgcggcctt cgtcgtcttc
      841 gtcgtcgtgc cgttcgggcc ggcgtggggc gtcatcacgg acatcaacat cgggttgctg
      901 ttcatcctct cggtctcctc ggtcggcgtg ctcgcgctga tcctcgccgg ttggtcttcg
      961 aactcgaagt acgcgctctt gggcggcctg cgctcgtcgg cgcagatggt ctcgtacgag
     1021 gtctcgatgg ggctgtcttt ggtgggcgcg ctgatgttcg cgcgcaccct ctcgatgtcg
     1081 ggcatcgtcg gggcgcaggc ggcggactcg gtctggttca tcctgtatca gccgctcggc
     1141 ttcgtcatct tcttggtgag cggcatcgcg gagaacaacc gcgcgccgtt cgacctgccc
     1201 gaggccgagt cggagctggt cgcgggcttc cacacggaat actccgggat gcgctggtcg
     1261 ctcttcttca tggccgagta cgcggcgatg atcgtggtgt cggcggtggc gaccgccgtc
     1321 tacctcggcg gctggtactt ccccggcgtc ttatggctcg cgggcggcta caacaacgtg
     1381 tgggtgctct tgagcctcct ggtcttcctc gccaagatgg cgctgctgct ctacctctat
     1441 ttctggatgc gctggacggt cccgcgctac cgctacgacc agttgatgga cttgggctgg
     1501 aagtggatga tcccggcctc gctcatcaac atcgtgtgga cgggcctcgc cgtcttcgcc
     1561 gtgcaggcgc tcgacggctg gcgcggcttc gacaccgtcg agtcgctcac gcgcggcctc
     1621 aacctgaccg cgacgggcaa ggccgtgatg atcgccttcg gtctcgcggg cgtccttctc
     1681 gccgccgcgc tgctctcggc cttcaactgg cgctcgcgcg acttcaacct gaagaagcag
     1741 cgccgc
//
LOCUS       DBNGOH010001185         5414 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_2196583, whole genome shotgun sequence.
ACCESSION   DBNGOH010001185 DBNGOH010000000
VERSION     DBNGOH010001185.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 5414)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5414)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5414
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_2196583"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3397
                     /locus_tag="AC159P_22945"
     CDS             <1..3397
                     /locus_tag="AC159P_22945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_process: GO:0098003 - viral tail assembly [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HIR3698359.1"
                     /translation="TRSVGEERAEEAANPPEMERPSGSPVTKQAPTDVPAAPSEGEGE
                     AEQEVAPEGEAQPVPEPEPLPPAPELPTQGVAAPQVTGNEQGEMSEEDVENLQDSISS
                     LPTNDPGLDVDAGEPPPLELEGDADPQRAAEQRANLEQSVADTHAQGQRDAAQPMGES
                     EIYPTVPKETLRAKVPEAGGGGGVAAAAGGGPAAAAMAGGEGGGGGDSAEAASIIAQE
                     KSGGEIQAAVSKAQGDMAAERVKHTEKVAEEKSKSQEEIAKAESENAAAQEAERSKAQ
                     GEVAEARADWSEEQQQVVDENQAQADEVTAEGEEEVEVQRVKGETEASTAIEEGNKEA
                     DTARADAEQKAESEKQKGEQESSGGFFGWLSSKATSFFNSIKAGIKAAFDAARKLVKA
                     AIEKAKQLAVAAIEAARKAIVDAIRKVGDALIALGDVLLAAFPGLREKWRNFIKEKVA
                     AAEAAVNKLAEDLKAGVQKLLDMLGAAIDAALGLLEKGLLAAVDAANAVVQGAIKAAQ
                     AVAEALGAFMVLVKDIASNPGGWIANLGSAAVDGVKNHLWGAFKTAVKQWFSDKVEEV
                     LGVGGMIWGVLKKGGIKMGEIGSMAWEAIKAAIPSVLISLIIEKVVSMIIPAAGAVLA
                     IIEGLQAAWGTVSRIIKAFSLFMAFLKAVKGGNAGPQFAQAVAAAAIVVIDFVANWLL
                     KRLRKPASKVAGKVKAMAQRILKKLKKAVSKVGKKIKKAFKKVGQKLKKIGAKLKGKL
                     KKLGGKLRKKKGKKPKKGKDKKKPDKSKEQKKRERLEKAVKAIQPKVEKLLSRGASSL
                     RLKAMLGLWKIGYRLSSLTLEPDGTINAAINPRMYVAKGVPIKDEELGKMLYPIFRAA
                     IEAAIREMKAAGQHTATEQSLIAGQGIPKGATRAEQIAALHGLPTSQLPAGPLKLTTD
                     LEAVSPHPFLLSGRVQVRGRQKQRTRESLPGSYAQGRARIRQQKRLGHTELARKGEAG
                     MKFLPIFESARDPGTAVPYALRDRMGIADPLHPNIPKGEQFHGEMPGKGKKGAAHQRR
                     GVLNPMAPIGAVDISRPRTKKVQAEIEFSVGVISRMVMEAARGKKAYAKAKGVPPGLA
                     QAMENFVRATQMTLKGKASYSKQELGVLKGQLTRQLKIFFKSL"
     gene            complement(3404..4771)
                     /locus_tag="AC159P_22950"
     CDS             complement(3404..4771)
                     /locus_tag="AC159P_22950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3698360.1"
                     /translation="MPKQPDSKLHFIPFKPTRGVFHKHRARGAGVAIALAELLSKHDF
                     PISIMNVSPAEAEDPSALLLQGELADREWAAVGGYYNSKELSPLKLVVVPPSATKPAG
                     EREFDFADDENLDGFIGDLREWLEEFGEGERAAPVDPLTPATPDQLVIDSWFYGLEWL
                     VDEITTVVDKEFLQTLRETLKFFSGRLGDPYADRRLRELVLLIIDPMQAKRFPEGEDD
                     DTLTEAMSMLTSQMNAAGTEDILPVLDEGVERGADPAAFHAARGYALLALDRAADAVE
                     DFEAVEKAGHARPALFGLGRAYVELGETDKATRALDRAIKTTKPGEPFNDIVRGTDAA
                     PEPDGRTWLGMLLAARGQAELSAGDFAAAEKSLRRALEEGTDESDALELLAAAYGEWA
                     TRNVERKPAEAKRLFKEQAAALERLYELSPRPEVVEDALSAADFLGDAALAKRWERRR
                     EESGA"
     gene            4985..>5414
                     /locus_tag="AC159P_22955"
     CDS             4985..>5414
                     /locus_tag="AC159P_22955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3698361.1"
                     /translation="MTIRNVYACLVHERQECVIDLVRNLRYLDPDSHILLYNGGNDAA
                     LLDGGFPWQSYRAVVHPRPRAMAWGRLHDFALDCMRFALDELPFDTLTIVDSDQLALR
                     AGYPARLSRFLADKRGVGMLGNSPAPQTWDTHIAPARAALR"
BASE COUNT          960 a         1803 c         1938 g          713 t
ORIGIN      
        1 cacgcgctcg gtcggcgaag agcgtgcaga ggaggcggcc aacccgcccg agatggagcg
       61 cccgtccggc tcgcccgtca ccaaacaggc cccgaccgac gtgcccgccg ccccgtccga
      121 gggcgagggc gaggccgagc aggaggtcgc gcccgaaggc gaggcgcagc ccgtgcccga
      181 acccgagccg ctgccgcccg cgcccgaact gccgacgcaa ggcgtcgccg cgccgcaggt
      241 caccggcaac gagcagggcg agatgtccga ggaggatgtc gagaacctgc aggattcgat
      301 cagcagcctg ccgacgaacg accccggcct cgacgtggac gcgggcgagc cgccgccgct
      361 cgaactcgaa ggcgacgcag acccgcagcg cgccgccgag cagcgcgcca acctcgaaca
      421 gtccgtcgcc gacacgcacg cgcaaggcca gcgcgacgcc gcgcagccga tgggcgagag
      481 cgaaatctac ccgaccgtcc cgaaggaaac gctgcgggcc aaagtgcccg aggcgggcgg
      541 cggcggcggc gtggcggcgg cggcgggcgg cgggccggct gccgccgcca tggcgggcgg
      601 cgaaggcggg ggcggcggcg acagcgccga ggccgcctcg atcatcgccc aagagaagag
      661 cggcggcgag attcaggccg ccgtcagcaa ggcgcagggc gacatggccg ccgagcgcgt
      721 caagcacacc gagaaggtgg ccgaagagaa gtcgaagtcg caggaggaga tcgcgaaggc
      781 cgagtcggag aacgccgcgg cgcaggaggc cgagcgcagc aaggcacagg gcgaagtcgc
      841 ggaggcgcgc gccgactggt cggaagagca gcagcaggtc gttgacgaga atcaggcgca
      901 ggccgacgag gtgaccgccg agggcgagga agaggtcgaa gtccagcggg tgaagggcga
      961 gacggaagcc tcgaccgcca tcgaggaggg caacaaggag gccgacacag cgcgcgccga
     1021 cgccgagcag aaggccgaga gcgagaagca gaagggcgag caggaatcga gcggcggctt
     1081 cttcggctgg ctctcgtcga aggctacgtc cttcttcaac tcgatcaagg ccggcatcaa
     1141 ggccgcgttc gacgccgccc gcaagctcgt caaggcggcc atcgagaagg cgaagcagct
     1201 cgccgtcgcg gccatcgagg ccgcgcgcaa ggccatcgtg gacgccatcc gcaaggtcgg
     1261 cgacgcgctc atcgcgctcg gcgacgtgct gctggcggcc ttccccggcc tgcgcgagaa
     1321 gtggcgcaac ttcatcaagg agaaggtcgc cgccgccgag gccgccgtca acaagctcgc
     1381 cgaagacctc aaggccggcg tgcagaagct gctcgacatg ctcggcgcgg ccatcgacgc
     1441 ggcgctcggc ctgctggaga agggcctgct cgcggcggtc gatgcggcca acgccgtcgt
     1501 gcagggggcg atcaaggcgg cgcaggccgt cgccgaagcc ctgggcgcct tcatggtgct
     1561 cgtcaaggac atcgcgtcca accccggcgg gtggatcgcc aatctcggct cggcggcggt
     1621 ggacggcgtg aagaaccacc tgtggggcgc gttcaagacc gcggtcaagc agtggttcag
     1681 cgacaaggtc gaggaggtgc tcggcgtcgg cggcatgatc tggggcgtcc tgaagaaggg
     1741 cggcatcaag atgggcgaga tcggctcgat ggcgtgggag gccatcaagg ccgcgatccc
     1801 gtccgtcctc atcagcctca tcatcgagaa ggtggtctcg atgatcatcc cggcggcggg
     1861 cgcggtgctc gccatcatcg agggcttgca ggccgcgtgg ggcacggtca gccgcatcat
     1921 caaggcgttc agcctcttca tggccttcct caaggcggtc aagggcggca acgccgggcc
     1981 gcagttcgcg caggcggtcg ccgccgccgc catcgtcgtc atcgacttcg tcgccaactg
     2041 gctgctcaag cggctgcgca agcccgcgag caaggtcgcg ggcaaggtca aggccatggc
     2101 ccagcgcatc ctcaagaagc tgaagaaggc cgtctcgaag gtcggcaaga agatcaagaa
     2161 ggctttcaag aaggtcgggc agaagctgaa gaagatcggc gcgaagctca agggcaagct
     2221 caagaagctg ggcggcaagc tcaggaagaa gaagggcaag aagccgaaga agggcaagga
     2281 caagaagaaa ccggataaga gcaaagagca gaagaagcgc gagcgtctgg agaaggccgt
     2341 caaggccatc cagccgaagg tggagaagct gctctcgcgc ggcgcgtcga gcctgcggct
     2401 caaggcgatg ctcggcctct ggaagatcgg ctaccgcctc tcatcgctca cactggagcc
     2461 ggacgggacg atcaacgccg ccatcaaccc gcggatgtac gtcgccaagg gcgtgcccat
     2521 caaggacgaa gagctgggca agatgctcta cccgatcttc cgcgcggcca tcgaggcggc
     2581 catccgggag atgaaggccg ccggccagca cacggcgacc gagcagtcgc tcatcgccgg
     2641 ccaggggata ccgaagggcg cgacgcgcgc cgagcagatc gccgccctgc acggtctgcc
     2701 gacctcgcaa ctgcccgccg ggccgctcaa gctgaccacc gaccttgagg cggtgtcgcc
     2761 ccatccgttc ctcctcagcg ggcgcgtcca ggtgcggggc cggcagaagc agcgcacgcg
     2821 cgagagcctg cccggctcgt acgcgcaggg ccgcgcgcgc atccgccagc agaagcggct
     2881 gggccacacg gagctggcgc gcaagggcga ggcggggatg aagttcctcc ccatcttcga
     2941 gtcggcccgc gaccccggca cggctgtccc ctacgcgctg cgcgaccgga tgggcatcgc
     3001 cgacccgctg cacccgaaca tcccgaaggg cgaacagttc cacggcgaga tgccgggcaa
     3061 ggggaagaag ggcgcggctc accagcgccg cggcgtcctc aacccgatgg cccccatcgg
     3121 cgccgtggac atttcgaggc cgcgcacgaa gaaggtgcag gcggagatcg agttctcggt
     3181 cggcgtcatc agccgcatgg tgatggaggc ggcccgcggc aagaaggctt acgccaaagc
     3241 caagggcgtg ccgcccggac tggcgcaggc gatggagaac ttcgtgaggg cgacccagat
     3301 gaccctcaag gggaaggcgt cctactcgaa gcaggaactc ggcgttctca agggacaact
     3361 gacccggcag ctcaaaatct tcttcaagag cctctagccg ggttcacgcg cccgactcct
     3421 cccggcgccg ctcccagcgt ttggcgaggg cggcgtctcc gaggaagtcg gcggcggaga
     3481 gcgcgtcctc gacgacctcg ggccgcgggc tgagttcgta gaggcgttcg agcgcggcgg
     3541 cctgctcttt gaaaaggcgt ttggcctccg ccggcttccg ctcgacgttc ctcgtcgccc
     3601 actcgccgta cgcggcggcg agcagttcga gcgcgtccga ctcgtccgtc ccctcttcga
     3661 gcgcccggcg caaagacttc tcggcggcgg cgaagtcgcc ggcgctcagc tcggcctgac
     3721 cgcgcgccgc caggagcatc ccgagccagg tgcgcccgtc cggctcgggc gcggcatccg
     3781 tcccgcgcac gatgtcgttg aaaggctcgc ccggcttcgt cgtcttgatg gcgcggtcga
     3841 gcgcgcgggt cgccttgtcg gtctccccga gttcgacgta ggcgcggccc agcccgaaca
     3901 gggccgggcg cgcgtgcccg gccttctcga ccgcctcgaa gtcttcgacc gcgtccgcgg
     3961 cgcggtcgag tgccagcagc gcgtagccgc gcgcggcgtg gaaggcggcg gggtcggccc
     4021 cgcgctcgac gccctcgtcc aacaccggga ggatgtcttc cgtgccggcg gcgttcatct
     4081 gcgaggtcag catgctcatg gcctccgtga gggtgtcgtc gtcctccccc tcggggaaac
     4141 gcttcgcctg catcgggtcg atgatgagga gaaccagctc gcgcaggcgc cggtcggcgt
     4201 aagggtcgcc gagccgcccg ctgaagaatt tcaacgtctc gcggagcgtt tgcaggaact
     4261 ccttatcgac gaccgtggtg atctcatcga cgagccattc gaggccgtag aaccacgagt
     4321 cgatgacgag ttggtcgggc gtcgccggcg tgagcggatc gaccggggcg gcgcgctcgc
     4381 cctcgccgaa ttcttcgagc cactcgcgca ggtcgccgat gaagccgtcg aggttttcgt
     4441 cgtccgcgaa gtcgaactcg cgctcgccgg ctggtttcgt ggccgacggc gggacgacga
     4501 ccagcttcag cgggcttagc tctttcgaat tgtagtagcc gccgacggcg gcccactccc
     4561 ggtcggccag ttcgccttgc agaagaagcg cggacgggtc ttccgcttca gcggggctga
     4621 cgttcatgat cgaaatgggg aaatcgtgtt tgctcaacag ctcggcgagc gcgatggcga
     4681 cgcccgcgcc gcgcgcgcgg tgcttgtgga agacgccccg cgtcggcttg aacgggatga
     4741 agtggagttt gctgtcgggt tgtttaggca tggtcgctcc tgcgccgtca gtgagccttc
     4801 gtcgaagtcc gcgcggtcgt tgcgtttcgg gggatgacgc cattatacac acgcgggaac
     4861 gaccggacga gacgacccgg ctgaaggggt gcggcgacgc gcgtcgcgcg ggtctccggg
     4921 gcgtagaaca aacacgaaag ggtgcgaggg gggaatgatt ctcctccgac cgcagggaca
     4981 agggatgact atccgcaacg tctacgcgtg cctcgtccac gagaggcagg agtgcgtcat
     5041 cgatctcgtg cgcaacctgc gctacctcga ccccgactcg cacatcctgc tctacaacgg
     5101 cgggaatgac gcggcgctgc tcgacggcgg cttcccgtgg cagagctatc gcgccgtcgt
     5161 gcacccgcgg ccccgcgcga tggcgtgggg gcggctgcac gacttcgcgc tcgactgcat
     5221 gcgcttcgcg ctcgacgagc tgcccttcga cactttgacc atcgtcgatt ccgaccagct
     5281 cgccctgcgc gcgggctacc cggcccgcct ctcgcgcttt ctcgcggaca agcggggcgt
     5341 cggcatgctc ggcaactcgc ccgccccaca gacgtgggac acgcacatcg ccccggcgcg
     5401 ggccgcgctc aggg
//
LOCUS       DBNGOH010001186         2409 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_365744, whole genome shotgun sequence.
ACCESSION   DBNGOH010001186 DBNGOH010000000
VERSION     DBNGOH010001186.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2409)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2409)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2409
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_365744"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(233..925)
                     /locus_tag="AC159P_22960"
     CDS             complement(233..925)
                     /locus_tag="AC159P_22960"
                     /inference="COORDINATES: protein motif:HMM:NF041414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsI/CadI family heavy metal resistance
                     metalloenzyme"
                     /protein_id="HIR3698362.1"
                     /translation="MESKTVQTLKAHLALNVRSVEASIGFYRKMFGIEPCKVRTGYAK
                     FDVANPPLNLTLNENPFGERGALSHLGVQVASTEDVLALRDRWREAGLSTFDEMQTNC
                     CYAIQDKTWVTDPDGNNWEAFVVLEDNLAETTLCCVADAAAGADAQGAALCSPTGAGE
                     SKAAPIKLKTSETVKAESKTAETASAETAAAAASCCATAENVAAASAPDTAVASSCCA
                     PATAEAPVSIGR"
     gene            1077..1565
                     /locus_tag="AC159P_22965"
     CDS             1077..1565
                     /locus_tag="AC159P_22965"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012959044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3698363.1"
                     /translation="MIESMRQGDWEGVRAVYVDGLATGEATFETEAPGWERWDASHLA
                     FARLVARAGETIEGWAALSPVSSRRVYAGVAEVSVYVGREARGRGLGRSLLEALIEES
                     ERHGVWTLQASIFPENASSVALHRACGFRVVGTRERVARLKGRWRDTVLLERRSRVVG
                     VD"
     gene            1589..1960
                     /locus_tag="AC159P_22970"
     CDS             1589..1960
                     /locus_tag="AC159P_22970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HIR3698364.1"
                     /translation="MELLFKALADRTRLRLLNLMGEDEVCVCYFVEVLGTNQPKISRH
                     LAYLRRAGVVSARRDGKWMHYRVVSPSDAHAARVFAEVRAWLREEREMQQDRTRLARI
                     CCAPQVPVRLQGAPRPASLAT"
     gene            complement(2094..>2409)
                     /locus_tag="AC159P_22975"
     CDS             complement(2094..>2409)
                     /locus_tag="AC159P_22975"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HIR3698365.1"
                     /translation="DWGRALGGVDLSRPSVLLSHEPAVLDMKETRGVSLILSGHTHGG
                     QISLPFVGAPARFMEEFKYLRGLYEREGTQLYVSRGTGMIGVPVRIGARPEVAVLRLR
                     RA"
BASE COUNT          358 a          856 c          765 g          430 t
ORIGIN      
        1 ggcggactct ccacgtcgaa cttcgcgtaa gcctcgcgca ccttgcacgg ccccgtgccg
       61 agcagcttcg tgtagaaccc gacgctcgcc tcgacgctcc gcacgttgat cgcgaggtgc
      121 ggcttcagcg cgcgcatttc cctccccgcc gtgtatccca cgattcggcc ctcctctcgc
      181 gccgcgtcca ttaacaacgc gcggagcgtc gttaatggac gcggctttct gttcaacgcc
      241 cgatcgagac cggcgcttcc gccgtcgcgg gcgcgcagca cgaagacgcg accgccgtgt
      301 ctggcgcgct cgccgccgcg acgttctccg ccgtggcgca gcacgaagcg gcggctgccg
      361 ccgtctccgc cgacgcagtc tccgcggtct tcgactcggc cttgacggtc tccgaggtct
      421 tcaacttgat cggcgcggcc ttcgactcgc ccgcgcccgt cggcgagcac agcgccgccc
      481 cttgcgcgtc ggcgcccgcc gcggcgtccg cgacgcagca cagggtcgtc tccgcgaggt
      541 tgtcttcgag cacgacgaac gcctcccagt tgttgccgtc cgggtcggtc acccacgtct
      601 tgtcctgaat ggcgtaacag cagttggtct gcatctcgtc gaaggtcgag aggcccgcct
      661 cgcgccagcg gtcgcgcagc gccagcacgt cctccgtcga ggccacctgc acgccgaggt
      721 gcgagagcgc cccgcgctcg ccgaacgggt tttcgttgag cgtcagattc agcggcgggt
      781 tcgccacgtc gaacttcgcg tagcctgtgc gcaccttgca cggctcgatg ccgaacatct
      841 tccggtagaa cccgatgctc gcctcgacgc tcctcacgtt cagcgccaaa tgggccttca
      901 acgtctgcac ggtcttgctt tccatctcgc ttctccttta caattcatat tcgcctaagc
      961 gaatttgaaa ggcagcatac gccccggcga catatatgtc aagacgtatt tgaaaaatat
     1021 tttgcgcgcc gtcgcgggcg aagaaagtga tgccgcgcgc gcgcgacgga tttcgtatga
     1081 tcgagtcgat gcgacagggc gattgggaag gcgtgcgcgc cgtctacgtg gatggactcg
     1141 ccacgggcga ggcgacgttc gagacggagg cgccgggatg ggagcgttgg gacgcttcgc
     1201 acctcgcgtt cgcgcgtctc gtcgcgcgcg cgggcgagac gatcgagggc tgggcggcgc
     1261 tcagcccggt ctcttcgcgc cgcgtctacg cgggcgtggc ggaggtgagc gtctacgtcg
     1321 gtcgggaggc gcgcgggcgc ggcctgggcc ggtctttgct cgaagcgttg atcgaagagt
     1381 ccgagaggca cggcgtctgg acgctgcaag cgagcatctt tccggagaac gcgtcgagcg
     1441 tcgcgctgca cagggcgtgc ggcttccgcg tggtcggcac gcgcgagcgc gtcgcccggc
     1501 tgaaggggcg ctggcgcgac acagttctgc tcgaacgccg cagccgggtc gtgggggtcg
     1561 attgaagatg gcgagtaagg gttcgcagat ggagctactg ttcaaggcgc tggccgaccg
     1621 cacgcgtttg cgcctgctga acctgatggg cgaagacgag gtgtgtgtct gctacttcgt
     1681 cgaggtgctc ggcacgaacc agcccaagat ttctcggcac ctcgcgtacc tgcgccgcgc
     1741 gggcgtcgtc tcggcgcggc gcgacggcaa gtggatgcac taccgcgtcg tctcgccttc
     1801 ggacgcgcac gcggcgcgcg tcttcgccga ggtgcgcgcg tggctccgcg aggagcgcga
     1861 gatgcagcag gatcggacgc ggctcgcgcg catctgctgc gcgccgcagg tgcccgtccg
     1921 cttgcagggg gcgccgcgcc cggccagcct cgccacctga accttctcgt ccgaaagggc
     1981 ctcaccgact tcatcgtctc tgtttttatc ttcctctttc tccgtgccct ctgtgcctac
     2041 tctgtgccct ccgtgttgaa acgaagctgc gccacactca accgccaacc gctctaagcc
     2101 cttcgcagcc ttagcactgc cacctccggc ctcgccccga tgcgcaccgg caccccgatc
     2161 atccccgtcc cgcgcgacac gtaaagctgc gtcccctcgc gctcgtacag cccccgcaga
     2221 tatttgaact cctccatgaa ccgcgccggg gcgccgacga acggcagact gatctgaccg
     2281 ccgtgcgtat gcccgctcag gatcagagac accccccgcg tctccttcat gtccaacacc
     2341 gccggctcgt gcgacagcaa gaccgacggg cgcgacaaat cgaccccgcc cagcgcacgc
     2401 ccccaatcc
//
LOCUS       DBNGOH010001187         3057 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_3298069, whole genome shotgun sequence.
ACCESSION   DBNGOH010001187 DBNGOH010000000
VERSION     DBNGOH010001187.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3057)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3057)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3057
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_3298069"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(137..484)
                     /locus_tag="AC159P_22980"
     CDS             complement(137..484)
                     /locus_tag="AC159P_22980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3698366.1"
                     /translation="MRLSFMVFTPSGLLMKGTADATFVADAFALASVHVRENIVASTI
                     LYAEESPAPRAPKEGEPGRPIYIARAVEIKSSARRALSLRGGPNFTPHETREGGDKFL
                     FTRFLQHSRSDGR"
     gene            468..1307
                     /locus_tag="AC159P_22985"
     CDS             468..1307
                     /locus_tag="AC159P_22985"
                     /inference="COORDINATES: protein motif:HMM:NF024206.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HIR3698367.1"
                     /translation="MKLRRITLLLLILCAALPLAARAQDAAKPDAKPDAAKQALHDQL
                     YEAARKGDAAEVKALLDKGADVNAKFRYGATALFKAAERGHTEVVRLLLERGADPKVQ
                     DTFYRATAMTWALDKKHLEVVRLLLDKDASAVDDVLLTGAREPDAAIVNLALGKGGAK
                     PETLTRALIVTTKAGKADLAEALKKAGAQPPPTLDAALLAAYAGKYKPEQGNELAVSV
                     ADGQLTATPTGQRPIDMMATDKTTFVPVAFDGITVTFVSEGDKVTGLTLKQGPNTTAY
                     KKQ"
     gene            1480..2856
                     /locus_tag="AC159P_22990"
     CDS             1480..2856
                     /locus_tag="AC159P_22990"
                     /inference="COORDINATES: protein motif:HMM:NF024753.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HIR3698368.1"
                     /translation="MKRLALSFACLCLVSAAARAQNWPQFRGAGATGVAEGKPAPVKW
                     DGEKSVGVRWKTPIPGLAHSSPVVWGNTVFVTTAVSSAAKDETRFGLYGDVEPVKDDP
                     AHTWKLYAVDKATGKILWERTAHTGLPKVKRHPKSTHADSTPATDGRTVVALFGSNGL
                     FAYDVKGKLLWKQDLGVLDAGWFYDPDYQWEHGSSPVIYKNLVIVQADIQKDSFIAAY
                     DLKTGKQVWKTMREGELSGWSTPTVYEGKAGPLLITSGAKAVRGYDPLTGKELWRLGP
                     MSEITTPTPFVAHDLIYVTSGYAPIQPIYAIRPEAKGDISLKDGKEANEFIAWSKQRG
                     GPYMPTPVVYGEHLYTVSNQGVVTAYDAKTGTRVYQERIAGKGGAFTASPVASDGKVY
                     LSSEDGEIFVFKTGAKHELLSTNPMGEVLMSTPAISDGMLIVRGTKHLFGIAESAPAA
                     APTAKAAK"
BASE COUNT          615 a         1047 c          945 g          450 t
ORIGIN      
        1 gacttttcgc gtcggggaga ccacggccac agtctaaaac atttaacgcc ggcgtgcagg
       61 tgttcgcggc acgcgcggcg ccgggcgtcg gcgccgcgct ctcgacaaaa ttccacgcct
      121 cttaatcaac gacacattat cgcccatcgg aacgcgaatg ttgtaaaaat ctcgtaaaaa
      181 gaaacttatc cccgccctcg cgcgtttcat gcggtgtaaa attcgggccg ccccgcagag
      241 agagtgcgcg ccgcgcgctc gacttgattt cgacagcccg cgcgatgtaa ataggtcggc
      301 ccggttcgcc ctctttcggc gcgcgaggcg cgggcgattc ttcggcatac aggatcgtgg
      361 acgcgactat attttcccgg acgtgaacgg acgcgagcgc aaaggcgtcc gccacgaacg
      421 tggcgtcagc cgttcccttc atcaacagac cgctcggagt gaagaccatg aagctcaggc
      481 gcatcaccct cctcctgctc atcctctgcg ccgcgctgcc cctcgcggcg cgcgcgcagg
      541 acgccgccaa gcccgacgcc aaaccggacg ccgccaagca ggcgctccac gaccaactct
      601 acgaggccgc gcgcaagggc gacgccgcgg aggtcaaggc gctgctggac aagggcgccg
      661 acgtgaacgc caagttccgt tacggcgcca ccgcgctctt caaggccgcc gagcgcggcc
      721 acaccgaagt cgtccggctc ctgctcgaac gcggcgccga cccgaaggtg caggacacct
      781 tctaccgcgc gacggcgatg acgtgggcgc tcgacaagaa gcacctcgaa gtcgtccgcc
      841 tgctgctcga caaggacgcg agcgccgttg acgacgtgct gctgaccggc gcgcgcgaac
      901 cggacgccgc catcgtcaac ctcgcgctcg gcaagggcgg cgccaagccc gagacgctga
      961 cccgcgcgct catcgtcacg accaaagcgg gcaaggccga cctcgccgag gcgctcaaga
     1021 aggcgggcgc gcagccgccg ccgacgctcg acgccgcgct gctcgcggcc tacgcgggca
     1081 agtacaagcc cgagcagggc aacgagctgg ccgtcagcgt cgccgacggc cagctcaccg
     1141 cgacccccac cggccagcgc cccatcgaca tgatggcgac cgacaagacg accttcgtcc
     1201 ccgtcgcctt cgacggcatc accgtcacct tcgtctccga aggcgacaaa gtcaccggcc
     1261 tcacgctcaa gcaggggccg aacacgacgg cgtacaaaaa acagtaggca gtaggcagca
     1321 ggcagttaaa gacaaagaca aggacgcacg attttcgaat cccgaattcg aaatcaagtc
     1381 gcttgtcatc atccaaccgc gaagcggaaa ttcaagatcg acaatgttcc gactgcctac
     1441 tgcctgctgt ctactgccta ctgctcagag aggttcaaga tgaaacgact cgcactttcc
     1501 ttcgcctgtt tgtgtctggt gtcggcggcg gcgcgcgcgc agaactggcc gcagttccgc
     1561 ggcgcgggcg cgacgggcgt cgccgaaggc aagcccgcgc ccgtgaagtg ggacggcgaa
     1621 aagtccgtgg gcgtccgctg gaagacgccg atcccgggac ttgcgcattc gagtccggtg
     1681 gtgtggggca acaccgtctt cgtcaccacc gccgtcagca gcgccgcgaa ggacgagacg
     1741 cgcttcggcc tctacggcga cgtggagccg gtcaaggacg accccgcgca cacgtggaag
     1801 ctctacgcgg tggacaaggc gacgggcaag attctgtggg agcgcaccgc ccacaccggc
     1861 ctgccgaagg tgaagcgcca cccgaagtcc acgcacgccg actcgacgcc cgcgaccgac
     1921 gggcgcaccg tggtcgcgct cttcggctcg aacggcctgt tcgcctacga cgtgaagggc
     1981 aagctgctgt ggaagcaaga cctcggcgtg ctcgacgcgg gctggttcta cgaccccgat
     2041 taccagtggg agcacggcag ctcgcccgtc atctacaaga acctcgtcat cgtgcaggcc
     2101 gacatccaga aagactcgtt catcgccgcc tacgatctga agaccggcaa gcaggtctgg
     2161 aagacgatgc gcgagggcga gctttcgggg tggagcacgc cgaccgtgta cgagggcaag
     2221 gccggccccc tgctgattac cagcggcgcg aaggcggtgc gcggctacga ccctctgacc
     2281 ggcaaagagc tgtggcggct cggccccatg tcggagatca cgacgccgac gcccttcgtc
     2341 gcccacgacc tgatctatgt caccagcggc tacgcgccca tccagccgat ctacgccatc
     2401 cggcccgagg cgaagggcga catctcgctc aaggacggca aggaggcgaa cgagttcatc
     2461 gcgtggagca agcagcgcgg cggcccctac atgccgacgc ccgtggtcta cggcgaacac
     2521 ctctacacgg tctcgaatca gggcgtcgtc acggcctacg acgcgaagac cggcacgcgc
     2581 gtctaccagg agcgcatcgc gggcaagggc ggcgcgttca ccgcctcgcc cgtcgcctcg
     2641 gacggcaagg tctacctgtc gagcgaggac ggcgaaatct tcgtcttcaa gaccggcgcc
     2701 aagcacgagc tgctctcgac caacccgatg ggcgaggtgc tgatgtcaac gcccgccatc
     2761 tccgacggca tgctcatcgt ccgcggcacg aagcacctct tcggcatcgc cgagagcgcg
     2821 cccgccgcgg cgccgaccgc gaaggccgcg aagtgattcg ctcaagatga aagttgaatg
     2881 aagtgagagc gtttatataa cgcagagttc gcggaggcgc gcagagggcg cagagaaaac
     2941 cgattcagga ttcgaggtat gaaatctttt cttctccgtg ctctctgcgt attctccgcg
     3001 aactctgcgt tagatcatcc gcccacgtcg ccgctcgacg cacattgaat ccagaaa
//
LOCUS       DBNGOH010001188         9371 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_549169, whole genome shotgun sequence.
ACCESSION   DBNGOH010001188 DBNGOH010000000
VERSION     DBNGOH010001188.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 9371)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9371)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9371
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_549169"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1301)
                     /locus_tag="AC159P_22995"
     CDS             complement(<1..1301)
                     /locus_tag="AC159P_22995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011556178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HIR3698369.1"
                     /translation="MSKRMSRLLAGLALCCGFFIFGGARDAAAATTYEVGPTKPFASI
                     GDVPWESLQPGDTVLIHWRSTPYREKWVICRQGTAEAPITVRGVAGPGGELPVIDGQS
                     ATTRLALDYWSETRGVIKIGGASIPADTMPRHIVIENLDIRNASTPFSFTDDGGGTQS
                     YTANASAIYVEKGEHITVRNCLIHDNGNGFFVASSDAAVSRDILVEGNHIYDNGNNGS
                     LFEHNNYTAAVGIVFQYNRFGPLRAGASGNNLKDRSAGTVVRYNWIEGGNRQLDLVDA
                     EDSSLIRADPRYRATHVYGNILIEPDAAGNRQIAHYGGDSGATATYRKGVLHFYNNTV
                     VSRRTDRTTLFRLSTNDERADARNNIFYVAAAAGSTLSLLDATGTLDLTHNWLKPGWV
                     ATFGTLSGTVNDDGTAVTGAAPGFADEAGQDYRLAAASACV"
     gene            complement(1420..3615)
                     /locus_tag="AC159P_23000"
     CDS             complement(1420..3615)
                     /locus_tag="AC159P_23000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 44 protein"
                     /protein_id="HIR3698370.1"
                     /translation="MIPRRLKALLRLALCACLLSSLPAARAQNPAVTINVDAAANRRA
                     INPNVYGVAHATSAQLSELHSPLNRHGGNNTTRYNWQLNADNRANDWYYQSIGDPSAV
                     AGERGDTFFAASRAAGAEPMLTVPLIGWVARLGANRSKLAGFSIAKYGAQTGNDWQWF
                     PDAGNGVRTTGQFVTGNDPHDANVPSDPAFQQGWVSHLVGRWGTNAAGGLRYYILDNE
                     PSIWHSTHRDVHPNGATMDEIRGRLIDYATMIKNVDPSALVVGPEEWGWSGYVFSGYD
                     QQYGGQHGWSFLPDRAAHGGRDYLPWLLDQMRQHDAATGRRLLDVFTVHYYPQGGEFS
                     NDTSSAMQLRRNRSTRSLWDPNYVDETWINDRVQLVPRLRNWVGTYYPGTLTGITEYN
                     WGAEGHINGATTQADIYGIFGREGLDMAARWTTPAASTPTFKAMKMYRNYDGQKSAFG
                     ETSVRAAAPNPDNLSAFAALRAGDGALTLLVVSKVLSGTTPVNVNLANFAPGPAAQVW
                     QLTASNTISRLTDITLNGATLSATLPAQSVTLFVIPAGDGNQPPVASASASPLSGLAP
                     LAVNFDGGASSDPDGTLASYIWDFGDGANAAGVTANHTYNTPGTYTARLTVTDNQGAT
                     ATATVTIVANADTPAAPNALTASAVSASRIDLAWTDSASNEQGFKVERCAGAGCTNFS
                     QIATVAAGVGAFSNNGLNKNTTYTYRVRAYNAAGNSAYSNTASARTLKR"
     gene            complement(3937..5955)
                     /locus_tag="AC159P_23005"
     CDS             complement(3937..5955)
                     /locus_tag="AC159P_23005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HIR3698371.1"
                     /translation="MRIIVSTVARSIRAGRSARPGHWTRNACALAAWCVLLHAATCVP
                     AAAATLPVGFTETQVAAGLSSPTAMAFAPDGRLFVCLQGGQLRVIKNGVLLPTPFVTL
                     SVSASGERGLLGVAFDPNFASNQFVYLYYTATTPTIHNRVSRFTANGDVAVAGSETTI
                     LELDNLSSATNHNGGAMHFGEDGKLYIAVGENANGANSQTLANLLGKMLRINPDGTIP
                     SDNPFFAQAVGRNRAIWALGLRNPFTFAFQPVTRRMFINDVGQNTFEEVNDGLAGANY
                     GWPESEGPTSNPGHRGPFYFYGHGSGLFGGCAITGGTFYNPATQQFPAEYVGDYFFAD
                     FCGGWINYLADPSNTNAVANFASGISAPVDLRVGPEGALYYLARGASSSSGVVFRIEF
                     TANQAPSITQHPANRTVSVGQSATFNVAASGTAPLAYQWQRGGVDIAGANSSSYTTPP
                     VLAGDNGARFRCVVSNSFGSATSNEAVLTVVSNQPPTGTITQPAVGALYSAGDTVTYA
                     GTATDPEQGTLPPSAFTWEVVFHHDTHTHPFIPPTSGATGGSFTIPRTGETSANVFYR
                     IHLTVTDAGGLTHASFRDVRPRTVTITLAASPTGPGLTLDGQPVAAPHTFVGVVGMLR
                     TIGAPSPQTVGRFTYTFRSWSDGGTQTHTITTPAANTTYTASFKKGKQ"
     gene            complement(6389..7264)
                     /locus_tag="AC159P_23010"
     CDS             complement(6389..7264)
                     /locus_tag="AC159P_23010"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3698372.1"
                     /translation="MKTVKAVVLISGLLALALAAPAARGGSPQTEEAAALNGAGIEHA
                     QQGRYKEAIECFERAIKLQPALAAPHNNLAVALIDLGRYGEAVAPLQQALRLRDGYPD
                     ATFNLGLAYLHLGKGGEAVALFERARRLMPSSFEAVINLGAAYNQAGDYPKAIEVLER
                     AVGIRPKDAAARNNLGAALFNAGRREDGVRAVQLAIQLDPACVEAYNNLGYFYMVMKR
                     HADAVGVLERAVRLDPSFASARYNLGAAYYAGGKKGHALEEYKKLQGMNEELATKLYF
                     VIFDRQIISVPETRR"
     gene            7451..8383
                     /locus_tag="AC159P_23015"
     CDS             7451..8383
                     /locus_tag="AC159P_23015"
                     /inference="COORDINATES: protein motif:HMM:NF015431.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR3698373.1"
                     /translation="MSLEIRHLKLIEAIAEEGNVTKAGGRLFLTQSALSHQLKDAEEK
                     LGTPLFVRLNKRMILTPAGERLLDSARLILGELRQAERDVKEISSTREGVMRICTECY
                     TCYHWLPSLLRSFNERHPRIEVQINVEATRQPVKSLIDGQLDLAVVSTPYRHARVTLR
                     PLFQDELLAVVRPGHPLASRHHAVAEDFADENLLVYQELEGGFFYEQVLRPAGVRPKR
                     TSQVQLTEAAVELVKAGLGVSVLARWVVAPYIKSGELRAVPITRKGLRRRWYAAVLKN
                     KDTPPHLLDFIELLVSGTPSAVASVETHTTRHVA"
     gene            8606..8764
                     /locus_tag="AC159P_23020"
     CDS             8606..8764
                     /locus_tag="AC159P_23020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3698374.1"
                     /translation="MGTSDGIRLDNFDASDPAAGGAAPKRMPQVSVSLLVRDLLLPVP
                     AARGLQGY"
     gene            8781..>9371
                     /locus_tag="AC159P_23025"
     CDS             8781..>9371
                     /locus_tag="AC159P_23025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3698375.1"
                     /translation="MKRPFHSILFLLLAVSVRATPLNVTGVQVSNRQYGTPAALRIYS
                     AGFAGTLIPTGAANPVASAQNEQAVRTGVNVAGRATAGTGSSADPWKGWESAVTSAQN
                     GVVHFPDGWYATSTGLNLYNRRNVRYTGGWGVVIKYTGPTGAGSAVVTFRADAGEPTL
                     PSSNIGIDFSGFIIEGGGAEYGLLLKSIGFNVFRNVR"
BASE COUNT         1461 a         2893 c         3336 g         1681 t
ORIGIN      
        1 ttcacacagg ccgacgccgc ggcgaggcgg tagtcctgcc cggcctcgtc cgcgaagccg
       61 ggcgccgcgc ccgtcaccgc ggtgccgtcg tcgttgaccg tgccggagag cgtgccgaag
      121 gtggcgaccc accccggctt gagccagttg tgcgtgaggt cgagggtgcc cgtcgcgtcg
      181 aggagcgaga gggtgctgcc cgccgccgcc gcgacgtaga agatgttgtt gcgcgcgtcc
      241 gcgcgctcgt cgttggtcga gaggcggaag agggtcgtgc ggtctgtgcg ccgcgagacg
      301 acggtgttgt tgtagaagtg gagcacgcct ttgcggtagg tggccgtcgc cccgctgtcg
      361 ccgccgtagt gcgcgatctg gcggttgccg gcggcgtccg gctcgataag gatattgccg
      421 tagacgtgcg tcgcgcggta gcgcgggtcg gcgcggatga gcgagctgtc ttcggcgtcc
      481 accagatcga gctggcggtt gccgccctcg atccagttgt agcggacgac ggtgcctgcg
      541 gagcggtctt tcaggttgtt accggaggcg cccgcgcgca aggggccgaa gcggttgtac
      601 tggaagacga tgccgacggc ggcggtgtag ttgttgtgct cgaagaggct gccgttgttg
      661 ccgttgtcgt agatgtggtt gccttcgacg aggatgtcgc gcgagacggc ggcgtcgctc
      721 gacgcgacga agaagccgtt gccgttgtcg tggatgagac agttgcggac ggtgatgtgc
      781 tcgcccttct cgacgtagat ggccgaggcg ttggcggtgt agctctgcgt cccgccgccg
      841 tcgtctgtga aggagaaggg cgtgctggcg ttgcggatgt cgaggttctc gataacgatg
      901 tggcgcggca tcgtgtcggc ggggatgctc gcgccgccga ttttgatgac gccgcgcgtc
      961 tcgctccagt agtcgagcgc gaggcgcgtg gtggcgctct ggccgtcgat cacgggcagc
     1021 tcgccgcccg gccccgcgac gccgcgcacc gtgatgggcg cttccgccgt gccctgccgg
     1081 cagatgaccc acttctctct gtagggggtc gagcgccagt ggatgagcac tgtgtcgccg
     1141 ggctgaagcg actcccacgg cacgtcgccg atgctggcga aaggcttcgt cggcccgacc
     1201 tcgtaagtgg tcgccgccgc cgcgtcgcgc gcgccgccga aaatgaagaa cccgcagcag
     1261 agcgccagcc ccgccaacag acgcgacatc ctcttgctca tccctgatcc acctttctcc
     1321 atcctgtgtg tactcactta tttcatcctg tcgtgcttgc ttgttccgaa ccggcgccgc
     1381 gcgaaggtgc ccctccaatc agggtgtact tcatcccgtt cacctcttga gcgtccgggc
     1441 gctggccgtg ttcgagtagg ccgagttgcc cgccgcgttg taggcgcgga cgcggtaggt
     1501 gtaggtcgtg ttcttgttga ggccgttgtt cgagaaggcg ccgacgcccg ccgcgacggt
     1561 cgctatctgc gagaagttgg tgcagcccgc cccggcgcaa cgctctactt tgaagccctg
     1621 ttcgttgctc gcactgtccg tccacgcgag atcgatgcgg ctcgcggaga cggcgctcgc
     1681 ggtgagggcg ttgggggcgg cgggcgtgtc cgcgttggcg acgatggtga cggttgcggt
     1741 cgcggtcgcg ccctgattgt ccgtcacagt caggcgcgcg gtgtaggtgc cgggcgtgtt
     1801 gtatgtgtgg ttggcggtga cgcccgcggc gttcgcgccg tcgccgaaat cccagatgta
     1861 tgaagcgagc gtgccgtcgg ggtcggacga cgcgccgccg tcgaagttga ccgcgagcgg
     1921 ggcgaggccc gagaggggcg aggcggaggc ggaggcgacg ggcggctggt tgccgtcgcc
     1981 tgcggggatg acgaagagtg tgacgctctg cgcgggcagc gtcgcgctca aggtcgcgcc
     2041 gttgagggtg atgtcggtga ggcgacttat cgtgttcgag gcggtcaact gccagacctg
     2101 tgccgcgggg ccgggcgcga agttggcgag gttgacgttg acgggcgtcg tgcccgaaag
     2161 gactttgctg acgaccaaca gggtgagcgc gccgtcgccc gcgcgcaggg cggcgaaggc
     2221 ggagaggttg tcagggttgg gcgcggcggc gcggacgctc gtctcgccga aggcggactt
     2281 ctggccgtcg tagttgcgat acatcttcat cgccttgaag gtcggcgtcg aggcggcggg
     2341 cgtcgtccag cgggccgcca tgtcgaggcc ctcgcgcccg aagatgccgt agatgtctgc
     2401 ctgcgtggtg gccccgttga tgtggccctc ggccccccag ttgtactcgg tgatgcctgt
     2461 gagcgtgccg gggtagtagg tgccgaccca gttgcgcagg cgcggcacga gctgcacgcg
     2521 gtcgttgatc cacgtctcgt ccacgtagtt cgggtcccag agcgagcggg tcgagcggtt
     2581 gcggcgcaac tgcatcgcgg acgacgtgtc gttcgagaac tctccgccct gtggatagta
     2641 gtggacggtg aacacgtcga gcaggcggcg gcccgtcgcc gcgtcgtgct gtcgcatctg
     2701 gtcgagcagc cacgggagat aatcccggcc gccgtgtgcg gcgcggtcgg gcaggaagct
     2761 ccagccgtgc tggcccccgt actgctggtc gtagccgctg aagacgtacc cgctccagcc
     2821 ccactcctcg gggccgacga cgagcgccga cggatcgacg ttcttgatca tcgtggcgta
     2881 gtcgatgagc ctgcccctga tctcgtccat cgtcgcgccg ttcgggtgca cgtcgcggtg
     2941 cgtggagtgc cagatgctcg gctcgttgtc gaggatgtag taacgcagac cgcccgcggc
     3001 gttcgtcccc cagcggccca cgaggtgcga gacccagcct tgctggaagg ccgggtcgga
     3061 aggcacgttc gcgtcgtgcg ggtcgttgcc ggtgacgaac tggccggtgg tacggacgcc
     3121 gttgccagcg tcgggaaacc actgccagtc gttgccggtc tgcgcgccgt acttggcgat
     3181 ggagaagcct gcgagcttcg agcggttggc gccgagccgc gcgacccagc cgatgagcgg
     3241 gacggtgagc atcggctccg cgcccgccgc gcgcgaggcg gcgaagaagg tatcgccgcg
     3301 ctcgcccgcc acggcgctcg ggtcgccgat ggactggtaa taccagtcgt tggcccggtt
     3361 gtcggcgttg agctgccagt tgtagcgcgt cgtgttgttg ccgccgtgac ggttgagcgg
     3421 gctgtgcagc tcggagagct gggccgaggt ggcgtgcgcg acgccgtaga cgttcgggtt
     3481 gatcgcgcgg cgattcgccg ccgcgtccac gttgatcgtg acggcggggt tctgcgcgcg
     3541 cgccgcgggc agggaagaca acaggcaggc gcagagggcg aggcgcagaa gcgccttcag
     3601 acgacgagga atcatgaaac gaaggctcct ataaaatttt tcggtgaggt ggtacgggct
     3661 tcaggcgggc ctgtcgggaa cacgcctctg aacaggtcta acttaccaca gcggccagcg
     3721 ccagttaaag aatgggttat cgggggaatt tccagagcaa gttccagagg ggtgcgggac
     3781 gtggtgaatc ctgtaggggc agggcttgtc cctgcccgtc aacatccgat tgattcaacg
     3841 ttggatgaaa tgtaaccgcg ggcagggaca agccctgccc ctacagttct cgcgggcgtt
     3901 cttcggttaa ctgaaacttg ttcgaaatcg cgtgccctat tgcttcccct tcttgaacga
     3961 cgccgtgtat gtggtgttcg ccgccggggt cgtgatggtg tgggtctgcg tcccgccgtc
     4021 cgaccacgag cggaaggtgt aagtgaacct gccgaccgtc tgcggcgagg gcgcgccgat
     4081 ggtgcgcagc atcccgacca cgccgacgaa cgtgtgcggc gccgccaccg gctggccgtc
     4141 gagcgtcagc cccggccccg tgggcgaggc cgccagcgtg atcgtcaccg tccggggccg
     4201 cacgtcgcgg aaggaggcgt gcgtcagccc gcccgcgtcc gtgacggtga ggtggatgcg
     4261 gtagaagacg ttggccgaag tctcgcccgt gcgcgggatg gtgaacgagc cgcccgtcgc
     4321 gccggaggtg ggcgggatga aggggtgcgt gtgcgtgtcg tggtgaaaga ccacctccca
     4381 cgtgaaggcg ctcggcggga gcgtcccctg ctcggggtcg gtcgccgtgc cggcgtaagt
     4441 gacggtgtcg cctgcgctgt agagcgcgcc cacggcgggc tgcgtgatgg tgccggtggg
     4501 cggttggttg gagacgaccg tgagcacggc ctcgttgctg gtggcgctgc cgaatgagtt
     4561 cgagacgacg cagcggaagc gcgcgccgtt gtcgcccgcc aagacgggcg gggtcgtgta
     4621 gctgcttgag ttcgcgcccg cgatgtcaac gccgcccctc tgccactggt acgcgagcgg
     4681 ggcggtgccg ctggccgcga cattgaaggt ggccgactgg cccacagaga ccgtccgatt
     4741 cgcggggtgc tgcgtgatgg agggtgcctg gttggccgtg aactcgatac ggaagaccac
     4801 gccggacgaa gacgacgcgc cgcgtgcgag gtagtagagc gcgccctctg ggccgacccg
     4861 cagatcgacc ggggcggaga tgcccgaggc gaagttggcc acggcgttgg tgttcgaagg
     4921 gtctgcgagg tagttgatcc agccgccgca gaagtccgcg aagaagtagt cgccgacgta
     4981 ctcggcgggg aactgctgcg tggccgggtt gtagaaggtg ccgccggtga tggcgcatcc
     5041 gccgaagagg ccgctgccgt ggccgtagaa gtagaacggc ccgcggtggc cggggttcga
     5101 ggtcgggcct tccgattcgg gccagccgta gttggcgccc gccagcccgt cgttgacctc
     5161 ctcgaaggtg ttctgcccca cgtcgttgat gaacatccgg cgcgtgaccg gctggaaggc
     5221 gaaggtgaag gggttgcgaa ggcccaaggc ccagatcgcg cggttcctac ccacagcctg
     5281 cgcgaagaag gggttgtcag aggggatggt gccgtcgggg ttgatgcgga gcatcttgcc
     5341 gaggaggttg gcgagcgtct gcgagttcgc cccgttggcg ttctcgccga cggcgatgta
     5401 gagcttgccg tcctcgccga agtgcatcgc cccgccgttg tggttcgtgg cgctggagag
     5461 gttgtcgagt tcgaggatgg tcgtctcgct cccggcgacg gccacgtcgc cgttggccgt
     5521 gaagcggctg acgcggttat ggatggtcgg cgtcgtcgcg gtgtagtaga ggtagacgaa
     5581 ctggttggag gcgaagttcg ggtcgaaggc caccccgagc agccccctct cgcctgacgc
     5641 gctgacgctc agcgtgacga agggcgtcgg cagcagcacc ccgtttttga tgacgcggag
     5701 ctggccgcct tgcaggcaga cgaagaggcg accatcgggc gcgaacgcca tcgccgtcgg
     5761 cgaagagaga ccggcggcca cctgcgtctc ggtgaagccc acggggagcg tggccgccgc
     5821 cgccggcacg cacgtcgccg cgtgcaggag cacgcaccac gcggccagcg cgcaggcgtt
     5881 gcgagtccaa tgaccggggc gggcgctgcg tcccgcacga atactccgcg cgacagtaga
     5941 cacgatgatt ctcatggatt tcgacctcgt ccgaagggtg agggtcaaca tatttaacgg
     6001 ggcgattgaa tacgtgacgg gggggaatta acggcgggat tatgtttcgc gtgccgggcg
     6061 atgtcaagga cgcctaactt cggggagtgc ctgaatgatg ggcgcttgaa cggttcactc
     6121 cgcaggccgt tgtcgctttc gcctcgtcat ccttatagcc gcgcgcgcaa cgcggcgacc
     6181 gtcatttctt ccggggccgg ccctgcgcaa cagcagcaag cgatggacaa tgccgatccc
     6241 gaaccggggc gcggcactcg aatagggcgg ccttccgaat cggtcgattc gccgccgtcc
     6301 aataccttcg atgttcccct gcctcctgac cgtcccggcg ggccttgtcc cctgcacgtg
     6361 agttcgcctg ctcgacgctc cgtggcgcct atcgccgagt ctcgggcacg cttattatct
     6421 gccggtcgaa gatgacgaag tacagtttgg tagccaattc ctcgttcatc ccttgcagct
     6481 tcttatactc ctcgagcgcg tgacccttct tcccgccggc gtaataggcc gccccgaggt
     6541 tataccgcgc cgaggcgaac gacgggtcga gtcggaccgc ccgctccagc acgccgacgg
     6601 cgtcggcgtg cctcttcatc accatgtaga agtaaccgag gttgttgtac gcctcgacgc
     6661 acgccgggtc gagttggatc gccagttgga cggcccgcac gccgtcctcc ctcctcccgg
     6721 cattgaagag ggccgcgccc aggttattac gcgccgccgc gtccttcggc ctgatgccga
     6781 ccgcccgttc gagtacttcg atggccttcg ggtagtcgcc ggcctgattg taagccgccc
     6841 ccaggttgat caccgcctcg aaggaggagg gcatcaggcg gcgcgccctc tcgaacagcg
     6901 ccaccgcctc gccacccttg ccgagatgca ggtaggccaa cccgaggttg aaggtcgcgt
     6961 ccgggtagcc gtccctgaga cgcagcgcct gttggagggg cgccacggcc tccccgtatc
     7021 tgcccaggtc tatcagcgcc accgccagat tattgtgcgg cgcggcgagc gcgggctgta
     7081 acttgatggc cctctcgaaa cattcgatgg cctccttgta ccgaccttgt tgcgcgtgct
     7141 cgatgcccgc cccattcagc gccgccgcct cttcggtctg gggggaacca cctcttgccg
     7201 ccggggccgc gagcgcgagc gcgagcaacc ccgaaatcag gacgaccgct ttcaccgttt
     7261 tcatcccaca tcctccgata aatcgcttgc aaccgccgag gcacgaggcc ggcgcggtct
     7321 cactgttttc ttttcgaaac cagtgtcgcc ggctacgggt tcatgctata gtcgttttat
     7381 ccagaaaaca aattgattgt tttctggata atgatgaatc ctattcatag taagtaggag
     7441 ggggtgagac gtgagcctgg agatcagaca cctcaagctg attgaggcga ttgccgagga
     7501 gggtaacgtg acgaaggcgg ggggcaggct cttcctgacg cagtcggcgc tcagccatca
     7561 gttgaaagac gcggaggaga aactcggcac gcccctcttc gtcaggctga ataagaggat
     7621 gatcctgacg cccgccgggg agaggctcct cgactcggcc cggctgatcc tgggggagct
     7681 gaggcaggcc gaaagggatg tcaaagagat ctcctcgacc cgtgagggcg tcatgcgcat
     7741 ctgcacggag tgctacacct gctaccactg gctcccctcg ctcctgcgct cgttcaacga
     7801 gcggcacccg cggatcgaag tccagatcaa cgtcgaggcg acgcggcagc ccgtcaagag
     7861 cctgatcgac ggccagttgg acctggcggt cgtctcgacc ccttaccgtc acgctagggt
     7921 gacgctgagg cccctctttc aggacgagct actggccgtg gtgaggcccg gccacccgct
     7981 cgcctcccgc caccacgccg tggcggagga cttcgccgac gagaatctat tggtctatca
     8041 ggaactggaa ggcggtttct tttacgagca ggttttgaga ccggcgggcg tgaggccgaa
     8101 gcgcacgtcg caggtgcaac tgaccgaggc cgccgtcgaa ctggtcaagg ccggacttgg
     8161 ggtcagcgtg ttggccaggt gggtggtagc gccgtacatc aaatccgggg agctgcgcgc
     8221 ggtcccgatc acgaggaaag ggctgcgccg gaggtggtac gccgcggtgc tgaagaacaa
     8281 ggacacgccg ccccacctcc tcgatttcat cgaactgctg gttagcggaa cgccgtccgc
     8341 cgtcgcgagc gtcgaaaccc acactactcg ccatgtagct tgaatgggaa acgacgaggg
     8401 ggcgactgca acgcgaatcg atagagcggg tcacgcgcca cccgtcgctc gacctcccgc
     8461 cgtgaagtcc ggagtagccg cccgagcgcc cgcggcaagg gcggccttca cgaccggtta
     8521 tacgcgggcg gcgtggagcg aatctcttcg accggcaacg ctatggccgc agcaggttaa
     8581 gtcgggcata ctgtcttaca gcttgttggg gacaagcgac ggcatacgcc tcgacaactt
     8641 cgacgcttcg gacccggcgg cgggaggcgc cgcgccgaag cggatgccgc aggtgagcgt
     8701 gagcctgctc gtgcgcgacc ttttgctccc ggtgcccgcc gcgcgcgggc tgcaaggtta
     8761 ctaagcgcga aaggagccag atgaaaagac cgttccattc aatcctgttc ctcctgctcg
     8821 ccgtgtcggt acgggctacc ccgttgaatg tgacgggggt tcaagtttcg aaccggcaat
     8881 acggcacgcc ggctgcccta agaatatact cggcggggtt cgccggtacg ctcatcccga
     8941 cgggtgccgc gaacccggtc gcctccgccc agaacgaaca ggccgtgcgc acgggcgtca
     9001 acgtcgccgg gcgcgcgacc gcgggcacgg gcagttccgc agacccttgg aagggctggg
     9061 agtcggctgt cacctcggcc cagaacggcg tcgttcattt ccccgacggc tggtacgcga
     9121 cctcgacggg gctgaacctc tacaacaggc gcaacgtccg ctacacgggc ggctggggcg
     9181 tcgtcatcaa gtacaccggc ccgacgggcg cgggttcggc tgtcgtcacc ttccgggcgg
     9241 acgccgggga gccgacattg ccgagttcga acatcggcat cgacttttcg ggcttcatca
     9301 tcgagggcgg gggcgcggag tacggcctgc tcctgaaatc catcggcttc aacgtcttcc
     9361 gcaacgtccg c
//
LOCUS       DBNGOH010001189         6182 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4946941, whole genome shotgun sequence.
ACCESSION   DBNGOH010001189 DBNGOH010000000
VERSION     DBNGOH010001189.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 6182)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6182)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6182
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4946941"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(106..2058)
                     /locus_tag="AC159P_23030"
     CDS             complement(106..2058)
                     /locus_tag="AC159P_23030"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HIR3698376.1"
                     /translation="MLFRLSDVHKSYGSQDVLRGASLQVNPGEHVGLVGRNGAGKTTL
                     FRMIGGEETPDKGDSVRARGLNLGLLEQHVHFEEGVTVHEAALAAFKNLQEIEHEMHA
                     LEHRMAEAPEDLDAVLERYSHLQHEFERQGGFEYAAKAEAILLGLGFGRETWSLETEK
                     LSGGQQNRLGLARLLLAEPDVLLLDEPTNHLDVNAVEWLEEFLASYKPAYVIISHDRY
                     FLDRCCTRIVEIELGRTASYTGNYSDYLVERDERREAQRRAYENQQHLIAKTEEFIRR
                     NLAGQKTKQAKSRRNMLQRMERVEAVPVDQSSGDFRLHKVERAGTNVLTVVEAAVGYP
                     GKLLADDITFTLRRAECLGIIGPNGSGKTTFLRTILNKIPALAGEIRWGTNVKIGYYA
                     QQLDDLDDRNEIIMELRRVALPSATAGELRTFLAKFLFTGDDVYKHVRDLSGGEKGRL
                     ALAKLIYSRVNVLVLDEPTNHLDIPSREALEEALDAYDGTIITISHDRYFLDRVATQI
                     LALDGRGTAEHYDGDYTEYHDWRAARRAAEAQARAAQEKGTADPVPPKGGRAAESGGG
                     KADAKSQGARGKKKAGARDPRLIEDEIAEAETRLAALSDEMSRPETARDPERSARLAD
                     DYRQADERLQSLIVEWERAAAEATSA"
     gene            complement(2173..3375)
                     /locus_tag="AC159P_23035"
     CDS             complement(2173..3375)
                     /locus_tag="AC159P_23035"
                     /inference="COORDINATES: protein motif:HMM:NF025010.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HIR3698377.1"
                     /translation="MHRFAKQFATLAVTALVALSAACKNSTSGSGTPDSAVAATVNGQ
                     NIMLSEVDRVISQQTNNQQDKMMPLELATARLQVLDGLIQQEVLFQRADKDKLLPNDD
                     EITQAINTQKQQANLTEEEYQKMLKESGQTEQQLRETARKQLAIQKLVEKTVSGVTVK
                     DSEVESFFNSNKERFVSPRGVAISAIFTDPRDSAGRYQDDAKSELDAKAKIDGIYQQL
                     RTGADFADVARRRSEDDTLVRGGDMGFWDEARLKQAGIPQEIVSALFGPMQPGQSTPP
                     TLLGDGRYVILKLTDRRLQAEPRTLESPGVRDDIKKALVDERKRILGEALRVVAVNEA
                     KVENFLAQSMIKDPSTLGGLQPATPTGGGASAPAATATPAASATASPAAAATAAPAVA
                     ASPSPATK"
     gene            3563..3757
                     /gene="rpmB"
                     /locus_tag="AC159P_23040"
     CDS             3563..3757
                     /gene="rpmB"
                     /locus_tag="AC159P_23040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018284253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HIR3698378.1"
                     /translation="MAKRCEVCGKGPQFGNNVSHANNRTRRRFDPNLQSIRVQRPQGG
                     NVRMKVCTTCIKAGKVAKAA"
     gene            complement(3862..4248)
                     /locus_tag="AC159P_23045"
     CDS             complement(3862..4248)
                     /locus_tag="AC159P_23045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HIR3698379.1"
                     /translation="MRRSVKTAEAPQAIGPYSQAIKAGGFVFTSGQIPLDPRTGEFVA
                     GGIAEQTERVLRNLSEVLRAAGTGLDKVMKTTVYLADMNDFAAMNEVYGAFFGEDPPA
                     RSTVEAARLPRDARVEIDVIALAGGE"
     gene            complement(4484..>6182)
                     /locus_tag="AC159P_23050"
     CDS             complement(4484..>6182)
                     /locus_tag="AC159P_23050"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HIR3698380.1"
                     /translation="ISHRLGMGRIRGELEDLAFQNLHPEDYRELSSQLEQRRHEHEAF
                     LGEITSTINEKLRESEVPVLRVEGRVKRLYSIWKKLRRQKITLEQVYDLIAARVVTPD
                     EVRHCYSALGVIHNNWQPIPGRFKDWIATPRDNLYQSLHTSVIGPKGQPFEIQIRTEE
                     MHRIAEEGVAAHWKYKEGKRGAREDDEAFQWLRSLVEWSQEVKDSRDFLESLKLDLYP
                     KDVYAFTPKGRVIQLPRGATPVDFAFAIHTEVGNKCTGARINKRMVPLRTAIQNGDVI
                     EILTSPNSKPSRDWLNFVKTSRARNRVRHAVAEQQRAESIELGRKLFEKEATRFQLPA
                     KKILNGNDGGDFKRIAGEYGYGSPDALLAAIGYGKVMPRNVIAKYLGPETFAEIEQTK
                     ETKIRSSVNAGVQAVKKLIRMGEDSIVVRGVDDLLVMRARCCNPVRGEEIVGYVTRGK
                     GVTVHARRCKNVQQLMVNPERIVEVEWAGSSDQSAYAVKLLAITENRTGMIAGITGAI
                     SDMKTGIRDARATVASDNRGRIEVTVEVFDVKHLDKVLSSIRKVPGVLDVERIQGAA"
BASE COUNT          893 a         2005 c         2094 g         1190 t
ORIGIN      
        1 aaacagcccc tccagcgctc ttctccggcg aacccggagg aggcgaagcc tccctcaccc
       61 acgcccgcgg caactcgtca gcagaccgaa gcccgaccgt gaggttcagg cgctcgtggc
      121 ttcggccgcc gcacgctccc actctacgat gagcgactgc aagcgctcgt cggcctgccg
      181 gtagtcatcc gcgagacgcg ccgaccgttc agggtcacgc gccgtctccg gccgcgacat
      241 ctcgtcggag agcgcggcga gccgcgtttc ggcctcggcg atctcgtcct cgatgagtcg
      301 ggggtcgcgc gcgcccgcct tcttcttccc gcgcgcgccc tgactcttcg cgtccgcctt
      361 cccccctccc gactcggcgg cccgtccgcc cttcggcggc accgggtcgg cagtcccctt
      421 ctcctgcgcc gcccgggcct gcgcctccgc ggcccggcgg gcggccctcc agtcgtgata
      481 ctcggtgtag tcgccgtcgt aatgctctgc cgtcccgcgg ccgtcgaggg cgagaatctg
      541 ggtggcgacg cggtcgagga agtagcggtc gtggctgatg gtgatgatcg tcccgtcgta
      601 ggcgtcgagc gcttcttcga gcgcctcgcg cgaggggatg tcgaggtggt tcgtcggctc
      661 gtcgaggacg aggacgttaa cgcgcgagta gatcagtttc gccagcgcga gccgcccctt
      721 ctcgccgccc gacaggtcgc ggacgtgctt gtacacgtcg tcgccggtga agaggaactt
      781 ggcgaggaag gtgcgcagct cgcccgccgt ggccgaaggc aaagcgacgc ggcgcagctc
      841 catgatgatc tcgttgcggt cgtcgaggtc gtcgagctgc tgcgcgtagt aaccgatctt
      901 gacattcgtg ccccagcgaa tctcgcccgc gagcgcggga atcttgttca gaatcgtccg
      961 caggaaggtc gtcttccccg agccgttcgg gccgatgatg ccgaggcatt cggcgcggcg
     1021 cagcgtgaac gtgatgtcgt cggccaggag cttgccgggg tagccgaccg ccgcctcgac
     1081 gaccgtcagg acgttcgtgc ccgcgcgctc gaccttgtgg aggcggaagt cgccggacga
     1141 ctgatcgacg gggacggcct cgacgcgctc catgcgctgg agcatgttgc ggcgcgactt
     1201 ggcctgcttc gtcttctggc ccgcgaggtt gcggcggatg aactcctctg tcttggcgat
     1261 gaggtgctgc tggttctcgt aggcgcggcg ctgggcctcg cgtcgttcgt cgcgctcgac
     1321 gaggtagtcc gagtagttgc ccgtgtagct cgccgtgcgc cccagctcga tctcgacgat
     1381 gcgcgtgcag cagcggtcga ggaagtagcg gtcgtggctg atgatgacgt aggccggctt
     1441 gtaagaggcg aggaactctt cgagccattc gacggcgttc acgtcgaggt ggttggtcgg
     1501 ctcgtcgagg agcagcacgt ccggctcggc gagcaggagc cgcgcaaggc cgaggcggtt
     1561 ctgctggccg cccgacagct tctcggtttc gagcgaccac gtctcgcgcc cgaagccgag
     1621 tccgagcagg atcgcctcgg ccttcgccgc atactcgaag ccgccctgcc gctcgaactc
     1681 gtgctgcagg tggctgtagc gctcgagcac ggcgtcgagg tcttcgggcg cctcggccat
     1741 gcggtgttcg agcgcgtgca tctcgtgctc gatctcttga aggttcttga aggccgcgag
     1801 cgccgcctcg tgcaccgtca cgccctcctc gaagtggacg tgctgttcga ggaggccgag
     1861 attcagcccg cgcgcgcgca ccgagtcgcc cttgtccggc gtctcctcgc cgccgatcat
     1921 gcggaagagc gtcgtcttcc ccgcgccgtt gcgccccacg aggccgacgt gctcgcccgg
     1981 attgacttgc agcgacgcgc cgcggagcac gtcctgcgac ccgtaggatt tatgaacgtc
     2041 actcagtctg aacagcatag aaccatcaac gaaggggaag tggcgaaaag gggaagaggc
     2101 gaaaaggtat tcgtctttcc cctcttcgcc ttttcccctt ttgccctttt ccccgaggct
     2161 gtgcggtggc gcttacttcg tcgccggaga gggcgacgct gcaacagccg gggccgcggt
     2221 cgcggcagcc gccggagacg cggtggcgga ggcagcaggg gtcgcggtgg ccgcgggcgc
     2281 ggaggcgccg ccgcccgtgg gcgtcgcggg ctgcaagccg ccgagcgtgc tcgggtcttt
     2341 gatcatgctc tgcgcgagga agttctcgac cttcgcctcg ttgacggcga cgacgcgcag
     2401 cgcctcgccg aggatgcgct tgcgctcatc gacgagcgcc ttcttgatgt cgtcgcgcac
     2461 gccggggctt tcgagggtgc gcggctcggc ctgcaagcgc cggtcggtca gcttgaggat
     2521 gacgtagcgc ccgtcgccga gcagggtcgg cggcgtggac tggccgggct gcatcgggcc
     2581 gaagagcgcc gagacgatct cctgcgggat gccggcctgc ttgagccgcg cctcgtccca
     2641 gaagcccatg tcgccgccgc gcacgagcgt gtcgtcctcg ctgcggcggc gggccacgtc
     2701 ggcgaagtcg gcccccgtcc gaagctgctg gtagatgccg tcgatcttgg ccttggcgtc
     2761 gagttcgctc ttggcgtcgt cctggtagcg gcccgcgctg tcacgcgggt cggtgaagat
     2821 ggcggagatg gcgacgccgc gcgggctgac gaaccgctcc ttgttcgagt tgaagaacga
     2881 ctcgacctcg ctgtccttca ccgtcacgcc gctcacggtc ttctcgacga gcttctggat
     2941 ggcgagctgc ttgcgcgccg tctcgcggag ctgctgctcg gtctgcccgc tctccttgag
     3001 catcttctgg tactcttcct cggtcaggtt ggcctgctgc ttctgcgtgt tgatggcctg
     3061 cgtgatctcg tcgtcgttcg gcagcagctt gtccttgtcg gcgcgctgga agaggacttc
     3121 ctgctggatc aggccgtcga gcacctgaag gcgcgcggtg gccagttcga gcggcatcat
     3181 cttgtcctgc tggttgttgg tctgctggct gatgacgcga tcgacttcgc tgagcatgat
     3241 gttctggccg ttgacggtgg cggcgaccgc cgagtcgggg gtgccgctgc cgctcgtgct
     3301 gttcttgcag gcggcggaga gggcgacgag cgccgtgacg gcgagggttg cgaactgttt
     3361 ggcgaatctg tgcactgtga ctttctgctc cttctgacta tctcttgtgt gatggccgtc
     3421 cccagcgaaa agcgtcgccg tccggcaggc ccgcttgacg ttacgcgaaa gcgtttgcta
     3481 tatatttttg tttccgatgc gcgccgccat cgtgactgtg cacgggcacg cactttttca
     3541 ttgttcggag gaatcaaacg tcatggccaa aagatgcgaa gtttgcggga aaggcccgca
     3601 gttcggcaac aacgtctcgc acgcgaacaa ccgcacgcgg cgccgcttcg accccaacct
     3661 gcaatccatc cgcgtccagc gcccgcaggg cggcaacgtc cggatgaagg tctgcacgac
     3721 ctgcatcaag gcgggcaagg tcgccaaagc ggcctgaact gacaaagagc ttagcgtctt
     3781 atcgagaagg cggcgggcgc ccccgttaac ggtcggcgcc cccgcttttc gtttgccgac
     3841 aactcaggcg cgaatcaaag atcactcgcc gcccgcgaga gcgatcacgt cgatctcgac
     3901 gcgcgcgtcg cgcggcagcc gcgccgcctc gacggtcgag cgcgcgggcg ggtcctcccc
     3961 gaaaaaggcc ccgtagactt cgttcatcgc ggcgaagtcg ttcatgtccg cgaggtagac
     4021 ggtcgtcttc atgaccttgt ccagccccgt gcccgcggcc ctcaaaactt ccgacaggtt
     4081 tcgcaagacc cgctcggtct gctccgcgat gccgcccgcg acgaactcgc ccgtccgcgg
     4141 gtcgagcgga atctggcccg aggtgaagac gaaccccccg gccttgatgg cctgcgaata
     4201 cggcccgatg gcctgcggcg cctccgctgt tttaacgctc cgcctcacgg tcacgacagt
     4261 ccctctccgc aacttcgcga acaaactccg gattttaaca gacactccac cgcacggcaa
     4321 acgacgaggc tgttagcggt tggctattag ctgtcagcta aaaatcaagc cggttattaa
     4381 ctttattgat gtttccttgc gcgaagggga tgcttggagg cgagacggaa gtacgaaaac
     4441 aatccgaagc tgacagctaa aggctaaccg ctaacagctc ctgtcacgcg gcgccctgga
     4501 tgcgctccac gtcgaggacg cccggcacct tgcggatcga ggagagcact ttgtcgaggt
     4561 gcttcacgtc gaagacctcg accgtgacct cgatgcgccc gcgattgtcc gaggcgaccg
     4621 tggcgcgcgc gtcgcggatg ccggtcttca tgtcggagat ggcgccggtg atgccggcga
     4681 tcatgcccgt gcggttctcg gtgatggcga ggagcttgac ggcgtaggcc gactggtcgg
     4741 agcttccggc ccactcgact tcgacgatgc gctcggggtt gaccatcaac tgctggacgt
     4801 tcttgcagcg ccgcgcgtgg acggtgacgc ccttgccgcg cgtgacgtag ccgacgatct
     4861 cttcgccgcg gacggggttg cagcagcgcg cgcgcatcac cagcaggtcg tccacgccgc
     4921 gcacgacgat ggagtcctcg cccatgcgga tgagcttctt gacggcctgc acgcccgcgt
     4981 tgacgctgct gcggatcttc gtctctttcg tctgctcgat ctcggcgaac gtctcggggc
     5041 cgaggtattt ggcgatgacg ttgcgcggca tcaccttgcc gtagccgatg gccgcgagca
     5101 gcgcgtcggg gctgccgtac ccgtattcgc ccgcgatgcg cttgaagtcg ccgccgtcgt
     5161 tgccgttcag gattttcttc gccgggagct ggaagcgcgt ggcctctttc tcgaagagct
     5221 tgcggcccag ctcgatgctt tcggcgcgct gctgttcggc gacggcgtgg cggacgcggt
     5281 tgcgcgcacg cgaagtcttg acgaagttga gccagtcgcg cgagggcttc gagttcggcg
     5341 aggtcaggat ttcgatcacg tcgccgttct ggatcgccgt acgcagcggc accatccgct
     5401 tgttgatgcg ggcaccggtg cacttgttcc cgacctcggt gtggatggcg aaggcgaagt
     5461 ccacgggggt cgcgccgcgc gggagctgga tcacccggcc cttcggcgtg aaggcgtaca
     5521 cgtccttcgg gtagaggtcg agcttcagcg actccaggaa gtcgcgcgag tctttcacct
     5581 cctgcgacca ctcgacgagc gagcggagcc actggaacgc ctcgtcgtcc tcgcgcgcgc
     5641 cgcgtttccc ctccttgtac ttccagtgcg cggcgacgcc ttcttcggcg atgcggtgca
     5701 tctcctcggt gcggatctga atctcgaagg gctgcccctt cgggccgatg acggaggtgt
     5761 ggagcgactg gtagaggttg tcacgcggcg tcgcgatcca gtccttgaag cggcccggga
     5821 tgggctgcca gttgttgtgg atgacgccga gcgcggagta gcagtggcgc acctcgtcgg
     5881 gcgtgacgac gcgcgcggcg atgaggtcgt agacctgttc gagcgtgatc ttctgccgcc
     5941 tgagcttctt ccagatcgag tagaggcgct tgacgcgccc ctcgacgcgc agcaccggca
     6001 cctcggactc gcgcagcttc tcgttgatgg tcgaggtgat ctcgccgagg aacgcctcgt
     6061 gctcgtggcg gcgttgttcg agctgcgagg acagctcgcg gtagtcttcg gggtggaggt
     6121 tctggaaggc gaggtcttcc agctcgccgc ggatgcggcc catcccgagg cggtgcgaga
     6181 tg
//
LOCUS       DBNGOH010001190         2786 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4397654, whole genome shotgun sequence.
ACCESSION   DBNGOH010001190 DBNGOH010000000
VERSION     DBNGOH010001190.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 2786)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2786)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2786
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4397654"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..824)
                     /locus_tag="AC159P_23055"
     CDS             complement(<1..824)
                     /locus_tag="AC159P_23055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3698381.1"
                     /translation="MCELNARKTYMWAITAGGAFAAAVSAYRLPLEQLDLKFAVLAVI
                     TVVASSRLTSKIPRLSSHITVSDTFIFLTMMLYGGEAAILLAAVEGLCSSLRVNKKVS
                     TTLFNSAVMVCSTFLTVATLRLFFGSIRELSHEGYTPLFVVAVCVMALAQYVFNSGLV
                     SVLAAYKIDRPFWHTWRNYYLWTSITYFAGASAAGIIAKLINSVGFYAVVVTTPIIAI
                     IFFTYETYRNHIEAAAAQAAQAERHVEELSLYIAEREQAERERDRLLVREREARAEA"
     gene            complement(849..2660)
                     /locus_tag="AC159P_23060"
     CDS             complement(849..2660)
                     /locus_tag="AC159P_23060"
                     /inference="COORDINATES: protein motif:HMM:NF012311.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HIR3698382.1"
                     /translation="MRHRLSNAQAGERVRVVIQPKAGWNDELDSVLRDHGASRESRFH
                     NFAARVVELPASAVANLEAHGDVAYVSLDREMRSLGHVSQTSGADAARAAHGATVSGL
                     DGTGVGIAVLDSGIDSSHRAFLDKSNNSRIVFSRDFTGEGRTDDPYGHGTHVASIAAG
                     NGRISNGQYTGVAPNANLINLRVLNSQGRGSVAGLLEALDWVLTNRAAYNIRVVNMSL
                     GGPAIDSYVDDPVCRAVRRLVDAGVVVLAAAGNNGRDGGGNKVYGQIHSPGNEPSAIT
                     VGASNSFGSDARGDDVVTSYSSRGPTRSHWTDADGVKHYDNLVKPDLVAPGNKLVYAQ
                     AAGNNLVAHNPALDAGVSPVDGRKMMYLNGSSMATPVAAGAAALLLQANPQLTPNLVK
                     MLLMYTAQPLAGFNQFEQGAGQLNVEGAVRLARLVRTDLTAATPLGAPLLTAAEAPAP
                     QTTISGHTFTWAQGIIVGHTYATGLDLITRYQRAYGLGLTLGSGVIIGDGHLAVDQAV
                     MSDGVIIGDHIMTGSGVIIGDGVPFLAVSQLLGGGVIIGDGVIIGDGVIIGDGVIIGD
                     GGLCADAVMQAQSATMNGDDTPSMPVEADGGVDYLDY"
BASE COUNT          431 a          919 c          999 g          437 t
ORIGIN      
        1 gcctcggcgc gcgcctcgcg ctcgcgcacc agcaggcggt cgcgctcgcg ctcggcctgc
       61 tcgcgctcgg cgatgtagag cgagagctcc tcgacgtggc gctcggcctg cgcggcctgc
      121 gcggccgcgg cctcgatgtg attgcggtac gtctcgtagg tgaagaagat gatggcgatg
      181 atcggggtgg tgacgacgac ggcgtagaag ccgaccgagt tgatgagctt ggcgatgatg
      241 cccgcggcgg acgccccggc gaagtaggtg atcgaagtcc aaaggtagta gttgcgccac
      301 gtgtgccaga aggggcggtc gatcttgtag gcggcgagca ccgagacgag gccggagttg
      361 aagacgtact gcgccaacgc catgacgcag accgccacga cgaagagcgg cgtgtagccc
      421 tcgtgcgaca gctcgcggat gctcccgaag aagaggcgca gcgtggccac cgtgaggaag
      481 gtcgagcaga ccatcaccgc cgagttgaag agcgtggtcg agaccttctt gttgacgcgc
      541 agcgaagagc agaggccctc gaccgccgcc agcaggatgg ccgcctcgcc gccgtagagc
      601 atcatcgtca ggaagatgaa ggtgtccgag acggtgatgt gcgagctgag ccgcggaatc
      661 ttgctcgtca ggcgcgagct ggccacgacc gtgatgacgg cgaggacggc gaacttgagg
      721 tcgagctgct ccagggggag gcggtacgcg gagaccgccg ccgcgaaggc gcccccggcg
      781 gtgatggccc acatgtaagt ctttctggcg ttcagttcac acatcacccc gcacctccat
      841 cccgcgcgtc agtagtccag gtaatcgacc ccgccatcgg cctcgaccgg catcgacggc
      901 gtgtcgtctc cgttcatggt cgcggactgc gcctgcatga cggcgtccgc gcacagcccg
      961 ccgtcgccga tgatcacccc gtcgccgata atcaccccgt cgccgatgat caccccgtcg
     1021 ccgatgatca cgccgccgcc gagcaactgg ctgacggcga ggaagggcac cccgtcgccg
     1081 atgatgacgc cgctgcccgt catgatgtgg tcgccgatga tgacgccgtc cgacatcacg
     1141 gcctgatcga cggcgaggtg cccgtcgccg atgatgacgc cgctgccgag cgtgaggccg
     1201 aggccgtacg cccgctggta tctggtgatg aggtcaaggc cggtggcgta ggtgtggccg
     1261 acgatgatgc cctgcgccca agtgaaggtg tggcccgaga tggtcgtctg cggcgcgggg
     1321 gcttccgcgg cggtgagcag cggggcgccg agcggggtcg ccgccgtcag atccgtgcgg
     1381 acgagccgcg cgaggcgcac cgcgccctcg acgttcaact gcccggcgcc ctgctcgaac
     1441 tggttgaagc ccgcgagcgg ctgcgccgtg tacatcagga gcatcttgac gaggttcggc
     1501 gtgagctgcg ggttggcttg caggagcagc gccgcggcgc ccgccgccac cggcgtcgcc
     1561 atcgacgagc cgttcaggta catcatcttc cgcccgtcca ccgggctgac gcccgcgtcg
     1621 agcgccgggt tgtgcgcgac gaggttgttg cccgcggcct gcgcgtagac cagcttgttg
     1681 ccgggcgcga cgaggtcggg cttgacgagg ttgtcgtagt gtttgacgcc gtcggcgtcc
     1741 gtccagtggc tgcgcgtggg gccgcgcgag ctgtagctgg tgacgacgtc gtcgccgcgc
     1801 gcgtcggagc cgaaagagtt cgaggcgccg accgtgatgg ccgaaggctc gttgccgggc
     1861 gagtgaatct gcccgtagac cttgttgccg ccgccgtccc tgccgttgtt gccggcagcc
     1921 gcgaggacga ccacccccgc gtcaaccaga cgccgcacgg cgcggcacac cgggtcgtcc
     1981 acgtaagagt ctatggccgg cccgccgagg ctcatgttga cgaccctgat gttgtaagcc
     2041 gcgcggttcg tcagcaccca gtcgagcgcc tcgagcaggc ccgcgacgct gcccctcccc
     2101 tgcgagttca gcacgcgcag gttgatgagg ttcgcgttcg gcgcgacgcc cgtgtactgg
     2161 ccgttcgaga tgcgcccgtt gccggcggcg atggaagcga cgtgcgtgcc gtgcccgtag
     2221 gggtcgtccg tgcgcccctc gcccgtgaag tcgcggctga agacgatgcg cgagttgttg
     2281 ctcttgtcga ggaaggcgcg gtggctactg tcgatgccgg agtcgagaac ggcgatgccg
     2341 acgcccgtgc cgtccagccc ggagacggtc gcgccgtgcg cggcgcgcgc ggcgtcggcc
     2401 ccgctcgtct gcgagacgtg gccgagcgaa cgcatttcgc ggtcgagcga gacgtaagcc
     2461 acgtcgccgt gcgcttcgag gttggcgacc gcactcgccg gcagctcgac cacccgcgcg
     2521 gcgaagttat ggaaacggct ctcgcggctc gcgccgtggt cgcgcaggac cgagtcgagt
     2581 tcatcgttcc accccgcctt cggctgaatg acgacgcgga cgcgctcgcc cgcctgcgcg
     2641 ttgctcaggc gatgccgcaa gtccggcgcg gtcttccgtc cgcgctcctg cggctggaca
     2701 gtccgggcgg acgctcgatc cccgacgaga atgcccgtgc acagtgaaat aagaagggcg
     2761 gataccagac cacagacgag gcttac
//
LOCUS       DBNGOH010001191         8112 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4213638, whole genome shotgun sequence.
ACCESSION   DBNGOH010001191 DBNGOH010000000
VERSION     DBNGOH010001191.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8112)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8112)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8112
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4213638"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..917)
                     /locus_tag="AC159P_23065"
     CDS             complement(<1..917)
                     /locus_tag="AC159P_23065"
                     /inference="COORDINATES: protein motif:HMM:NF024867.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR3698383.1"
                     /translation="MHVTRVELENIKSYERRAFDFERGTTAIVGPNGAGKTTILEAVA
                     WALFDVLDYSKDDFLRRGARRGSVRVTFQSDLDERRYTVYRDTGSGYYVYDPELRVKL
                     QQGKADVRKFLEQHLGVEPGTDVQALFKSAIGVPQGTFTADFLKSKTQREAAFNGLLK
                     VEEYRESADRLRDTVNLVNERIAEARGRIGGAESQLARYDELVAERKATGARVEELDA
                     TVQSFVSKAEEHERAVALMDEAERRVAETRARSDRLEVEHGAARRRLLELRGDLEAAR
                     RAAERRRATEEDYAAHRAALDELRALETER"
     gene            complement(1078..1539)
                     /locus_tag="AC159P_23070"
     CDS             complement(1078..1539)
                     /locus_tag="AC159P_23070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3698384.1"
                     /translation="MGTVLGRFVKVLTFTAVLAVLGPVLIVAALYFITGSTPNLEGAS
                     AEQLAGLVKALGVWFGVMLLIGFVLAIIGMSRKFTVPFSDRDAFIARLDDAAKSVRYR
                     PQSRDGDRLVYKPPFIQRLAEKIHVEVGANEATVNAPLNLSGKLRKKLREG"
     gene            complement(1784..3451)
                     /locus_tag="AC159P_23075"
     CDS             complement(1784..3451)
                     /locus_tag="AC159P_23075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3698385.1"
                     /translation="MKFPLTVLALFLSCSAAFAQHDHHNHGAAATAKPEVVRLLPELG
                     AFHFPVANSNAEAQKFFDQGVILVYGFNHDEAARSFRRASQLAPLMPMAHWGIAMALG
                     PNYNEKNIPPARLKAAHEAAQWGLALAQSAKAPAHERAYLEALAKRFSADANAEQSKL
                     WTAYRDAMRELMTKYPDDLDAATLWADAAMIINAWKLWSPQGRPAEGTEEIVAVLESV
                     LRRDPTHIGAHHLYIHAVEASPYPERATQSADLLAKLSPAAGHLVHMPAHTYMRVGDY
                     EAAARSNEWAAKADEEYLANGGLRGVYSAGYYSHNLHFLAAAHSMQGRYADAVKASRR
                     LEENVRPYLKDIPSLEGFMPTGVLIMARFGRWEDVLKEPEPAQSMPVTGALWHWARGM
                     AHAAKGKAAEAEAERKTLAAAAAAIPAEMNYGPQNTARSVLKIAEHFLDSRIAAARGD
                     RRGAVESLRQAVEAEDALAYDEPPGWYHPLSRESLGGALLLDKQYAEAEAVFREDLRR
                     NRRNGRSLFGLAESLKAQGKTREADLVRREFERAWRNADTQLKVEDL"
     gene            complement(3477..5276)
                     /gene="lepA"
                     /locus_tag="AC159P_23080"
     CDS             complement(3477..5276)
                     /gene="lepA"
                     /locus_tag="AC159P_23080"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020712834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HIR3698386.1"
                     /translation="MKPDRIRNFSIIAHIDHGKSTLADRLLELTGAVAGRDMEAQVLD
                     NMDLERERGITIKAHAVRLDYRANDGQQYVLNLIDTPGHVDFTYEVSRSLSACEGALL
                     VVDASQGVEAQTLANTYLAIANNLELIPILNKIDLPGAEPEKAIEQIENVIGLDTHNA
                     VLASAKTGVGVPEILEAIVRDIPAPKGDPEAPLKALIFDSWYDSYRGVIVLFRVIDGA
                     IKPGMKIRFFNTGRDYQVETIGVNRPRPTPIDRLSVGEVGFLTASIKTVADVQIGDTI
                     TEAARPTSVPFPGFQEVKPMVFAGLYPVEANQYEELRDALDKLRLNDASFFYEPESSV
                     ALGFGFRCGFLGLLHMEIVQERLEREFNLDLITTAPGVRYRVTTTDGEVIEVDSPAKM
                     PDAVRISKIEEPIIEATILSADEYLGGILPLLEEKRGVQKKFEYIAEKRVMLVYELPL
                     NEIVLDFYDRLKSVSRGYASLDYHFAEYWESDLVKLDLMVAGEPVDALSIIVHRGTSQ
                     ARGRLLAEKMRQLIPRQLFEVPIQATIGNKVIARETVKAMGKNVIAKCYGGDISRKRK
                     LLEKQKEGKKRMKRVGRVEIPQEAFLAVLKVNE"
     gene            complement(5524..6642)
                     /locus_tag="AC159P_23085"
     CDS             complement(5524..6642)
                     /locus_tag="AC159P_23085"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3698387.1"
                     /translation="MRLSFIWGVWTLVGLFFASQLYVVASRSGSERPIPFSRALVWQM
                     GACYLFALLTPLVLWLSRRFRIERANWRRAVLVHAAAGTLIAVAWGAGHILLDTWFSK
                     DFSKLTLDRLLRLTFFMLDKELLVYWVIVLISHAATYYQRYREGELRASQAQLQALKM
                     QLHPHFLFNALHSISALVHTDAEAADRMIARLGDFLRLTLETSATQEVPLRQELEFMN
                     CYLDIERVRFHDRLTTRVEIEPQALNCRVPNLILQPLVENAIRHGIALRSTPGRVEVR
                     AARRGGVLRLEVGDNGPGLPADEAHITSDGHGVGLANTRARLAQLYGAAYSLEMTNDP
                     RGGLLISIEIPAASGDELPAAVPRREIPGHGYVPALSK"
     gene            complement(6827..7051)
                     /locus_tag="AC159P_23090"
     CDS             complement(6827..7051)
                     /locus_tag="AC159P_23090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3698388.1"
                     /translation="MPFVSATREDGATLLINLDRIQYVAYQDTEGQEVLSIVFDTNPP
                     AAGRPGTNEIVVHGEEARRVWQALGALLGL"
     gene            complement(7122..7844)
                     /locus_tag="AC159P_23095"
     CDS             complement(7122..7844)
                     /locus_tag="AC159P_23095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5131 family protein"
                     /protein_id="HIR3698389.1"
                     /translation="MGSKSTIEWMESTWNPVTGCTKISPGCDHCYAERLALRLRAMGQ
                     PNYVNGFELTIQERMLELPLRWKTPQVIFVNSMSDLFHRDVPTDYIHKVFNVMNHACQ
                     HRFQVLTKRSQRLLRLSTALEWRPHVWMGVSVESKDYTFRIDHLRETGAHIKFLSLEP
                     LLGPLPNLNLKGIDWVIVGGESGPGARIMRPEWVVDVKDQCKAAGVPFFFKQWGGTNK
                     KRSGRLLENKTWDEMPRAVAVA"
BASE COUNT         1366 a         2495 c         2721 g         1530 t
ORIGIN      
        1 cgttcggttt cgagcgcgcg tagctcgtcg agcgcggcgc ggtgcgcggc gtaatcttct
       61 tcggtcgcgc ggcggcgttc ggcggcgcgg cgcgcggctt cgaggtcgcc gcgcagctca
      121 agcaggcgac gacgggcggc cccgtgctcg acttcgagcc ggtcgctgcg cgcgcgcgtc
      181 tccgcgacgc ggcgctcggc ctcgtccatc aaggcgacgg cgcgctcgtg ctcttcggct
      241 ttgctcacga agctttgcac cgtcgcgtca agctcctcga cgcgggcgcc cgtcgccttg
      301 cgctcggcga ccagctcgtc gtagcgcgcg agctgcgact ccgcgccgcc gatgcgcccg
      361 cgcgcctcgg cgatgcgctc gttgacaagg ttcacggtgt cgcgcaaacg gtcggcagat
      421 tcgcggtact cttcgacttt aaggaggccg ttgaaggccg cctcgcgctg ggttttggac
      481 ttgaggaagt cggcggtgaa ggtgccctgc gggacgccga tggccgactt gaagagcgcc
      541 tgcacgtccg tgcccggctc gacgccgagg tgctgttcga ggaacttgcg cacgtcggcc
      601 ttgccctgct ggagtttgac gcgcagctcc gggtcgtaga cgtagtagcc gctgccggtg
      661 tcgcggtaga cggtgtatcg ccgctcgtcg aggtcgctct ggaaggtgac gcgcacgctc
      721 ccgcgcctgg cgccgcgccg caggaagtcg tccttgctgt agtcgagcac gtcgaacagc
      781 gcccacgcga ccgcttcgag gatggtcgtc ttgcccgcgc cgttcggccc gacaatcgcc
      841 gtcgtcccgc gctcgaaatc gaacgcgcgg cgctcgtacg acttgatgtt ctccagttcg
      901 acccgcgtga cgtgcatctg tgtggattgc ggattgcgga ttgcggattg cggattgaag
      961 aggaacagag ggttgatgag gcgacgctct catcgctcga ccgatgcctg cggcgctcgc
     1021 atcgaccgcc aacgcttctt caatccgcaa tccgcaatcc gcaatccaaa atctttctca
     1081 tccttccctg agtttcttcc tgagcttgcc cgacaggttc aagggcgcgt tgaccgtggc
     1141 ttcgttcgcg ccgacctcga cgtgaatctt ttcggcgagc cgttggatga agggcggctt
     1201 gtagacgagg cggtcgccgt cgcggctctg gggccggtag cggacgctct tggccgcgtc
     1261 gtccagccgc gcgatgaagg cgtcgcggtc gctgaagggc acggtgaact tccgcgacat
     1321 gcctatgatg gcgaggacaa agccgatcag cagcatcacg ccgaaccaca cgccgagcgc
     1381 cttgacgagt cccgcgagtt gctccgcgct ggcgccttcg aggttgggcg tgctgccggt
     1441 gatgaagtag agcgcggcca cgatgagcac ggggccgagc accgcgagga cggcggtgaa
     1501 ggtcaacacc ttgacgaacc tgccgagaac agtccccatc cttccggtct cctccgtctg
     1561 aagttttcgg gattcggcgc cgccgcgctc gcttcgaggc ggccaggggc gcgcggcggc
     1621 gtccgggcga agggagagtc taaggcatcg tcgggccgat ggccagtgag cgacctcggg
     1681 cggaagggga aaaggaagaa ggggcgaaaa ggtaaaaagg cgaaaaggaa aagaagttgt
     1741 cttctccctt ttcccccttc gccattcccc ttcgttcggg gccttacaaa tcctcgacct
     1801 tgagctgcgt gtcggcgttc ctccacgcgc gctcgaactc gcggcggacg aggtcggcct
     1861 cgcgcgtctt gccttgcgcc ttgaggctct cggcgaggcc gaagagcgag cgaccgttgc
     1921 ggcggttgcg gcgcaggtct tcgcggaaga cggcctcggc ctccgcgtac tgcttgtcga
     1981 gcagaagggc gccgccgagg gattcgcgcg agagcgggtg ataccagccg ggcggctcgt
     2041 cgtaggcgag cgcgtcctcg gcctcgaccg cctgacgcag agactcgacg gcccctcggc
     2101 ggtcgccgcg cgccgccgcg atgcgcgagt cgaggaagtg ctcggcgatc ttaagcaccg
     2161 agcgcgccgt gttctgcggg ccgtagttca tctcggcggg gatagccgcg gcggcggcgg
     2221 cgagggtttt ccgctcggcc tcggcctccg ccgccttgcc cttcgcggcg tgcgccatgc
     2281 cgcgcgccca gtgccagagc gcgcccgtga cgggcatcga ctgcgccggc tccggctcct
     2341 tcaacacgtc ttcccagcgc ccgaagcgcg ccatgatgag cacgcccgtc ggcatgaagc
     2401 cttcgagcga ggggatgtct ttcagatagg gccgcacatt ctcttcgaga cggcggctcg
     2461 ccttgacggc gtcggcgtag cgcccctgca tgctgtgcgc ggcggcgagg aagtgcaggt
     2521 tgtggctgta gtacccggcg gagtagacgc cgcgcaggcc gccgttcgcg agatactctt
     2581 cgtccgcctt cgccgcccac tcgttcgagc gcgccgcggc ctcgtagtcg ccgacgcgca
     2641 tgtaggtgtg cgcgggcatg tggacgaggt ggccggcggc gggcgagagc ttggccagaa
     2701 ggtctgcgct ctgggtcgcg cgttcggggt agggcgaagc ctcgacggcg tggatgtaga
     2761 ggtggtgcgc gccgatgtgc gtcgggtcgc ggcgcaggac ggattcgagc acggcgacga
     2821 tctcttcggt gccttcggcg ggccgcccct gcggactcca cagcttccac gcgttgatga
     2881 tcatcgcggc gtccgcccac agcgtcgccg cgtcgaggtc gtccgggtac ttcgtcatca
     2941 gttcgcgcat cgcgtcgcgg taagccgtcc acagcttcga ctgctccgcg ttcgcgtccg
     3001 ccgagaacct cttcgccagc gcttcgaggt acgcgcgctc gtgcgcgggc gccttcgcgg
     3061 actgcgcgag cgcgagtccc cactgcgccg cctcgtgcgc cgccttcaaa cgcgcgggcg
     3121 ggatattctt ctcgttgtag ttggggccga gcgccatcgc gatgccccag tgcgccatcg
     3181 gcatcaaggg cgcgagctgc gaggcgcggc ggaacgagcg cgccgcctcg tcgtggttga
     3241 acccgtagac gaggatcacg ccctgatcga aaaacttctg cgcctcggcg ttcgagttcg
     3301 ccacggggaa gtggaacgcg ccgagttcgg ggagcaggcg cacgacctcc ggcttcgccg
     3361 tcgccgcggc gccgtggttg tggtgatcgt gctgcgcgaa cgccgcacta caggacagaa
     3421 acaacgcgag gacagtcagg ggaaacttca tctcgcactc tcctgatgtg gaagggttac
     3481 tcgttgacct tgaggactgc gaggaacgcc tcttgcggga tttcgacgcg gcccacgcgc
     3541 ttcatgcgct tcttgccctc tttctgtttt tcgaggagct tgcgcttgcg cgagatgtcg
     3601 ccgccgtagc acttggcgat gacgttcttg cccatcgcct tgacggtctc gcgcgcgatg
     3661 accttgttgc cgatggtggc ctggatgggg acttcgaaga gctggcgggg gatgagctgg
     3721 cgcatcttct cggcgagaag gcgcccgcgc gcctgcgagg tgccgcggtg gacgatgatg
     3781 gagagcgcgt ccaccggctc gcccgcgacc atcaggtcga gcttgacaag gtcggactcc
     3841 cagtattcgg cgaagtgata atccaatgaa gcgtagccgc gcgagaccga cttcagccgg
     3901 tcgtagaagt cgaggacgat ctcgttcaag ggcagctcgt agacgagcat gacgcgcttc
     3961 tcggcgatgt actcgaactt cttctgcacg ccgcgcttct cttcgagcag ggggaggatg
     4021 ccgccgaggt actcgtccgc cgagaggatg gtcgcctcga tgatcggctc ttcgatcttc
     4081 gagatgcgca ccgcgtcggg catcttcgcg ggcgaatcga cctcgatcac ctcgccgtcg
     4141 gtcgtcgtca cgcggtagcg gacgcccggc gccgtcgtga tgaggtcgag attgaactct
     4201 ctttcgagtc gctcctgcac gatctccatg tggaggagtc ccagaaagcc gcagcggaag
     4261 ccgaagccga gcgcgaccga gctttccggc tcgtagaaga aggaggcgtc gttgagccgc
     4321 agcttgtcga gcgcgtcgcg cagctcttcg tactggttcg cttcgaccgg gtagaggccg
     4381 gcgaagacca tcggcttgac ttcctgaaag ccggggaagg ggacggaagt cgggcgcgcg
     4441 gcctcggtga tggtgtcgcc gatctgcacg tcggccaccg tcttgatcga ggcggtgagg
     4501 aagccgacct cgcccaccga gaggcggtcg atgggcgtcg ggcgcgggcg gttgacgccg
     4561 atggtctcga cctgatagtc gcggcccgtg ttgaagaaac gaatcttcat gccgggtttg
     4621 atcgcgccgt cgatgacgcg gaacagcacg atcacgccgc ggtaggagtc gtaccacgag
     4681 tcgaagatga gcgccttcag cggggcctcg gggtctcctt tgggcgcggg gatgtcgcgg
     4741 acgatggctt ccagaatctc gggcacgccg acccccgtct tcgcggaggc gagcacggcg
     4801 ttgtgcgtgt cgaggccgat cacgttctcg atctgctcga tggccttctc gggttccgcg
     4861 cccggcaggt cgatcttgtt gaggatgggg atgagttcga ggttgttggc gatggcgagg
     4921 taggtgttcg ccagcgtctg cgcctcgacg ccctgcgagg catcgacgac gaggagcgcg
     4981 ccctcgcacg ccgacagcga gcgcgagacc tcgtacgtga aatcgacgtg gcccggcgtg
     5041 tcgatgaggt tgaggacgta ctgctgcccg tcgttcgcgc ggtagtcgag gcggacggcg
     5101 tgcgccttga tggtgatgcc gcgctcgcgc tcaaggtcca tgttgtccag aacctgcgcc
     5161 tccatgtcgc gcccggcgac ggcgcccgtc agctccagca gccggtcggc gagcgtggac
     5221 ttgccgtggt cgatgtgcgc gatgatggaa aagttcctga tgcgatctgg cttcattaaa
     5281 agtaggccgt cgagttcacc tctggattgc ttggcctaac ataacaaaac gggcgtgaaa
     5341 ccgcacgcgc gaggccggga gcgaagggga cggcgatcag ccgcggagcc acggagatga
     5401 agaaagcaat aaaggaagag acgcgacgag ggagagtcga atcgttcaac cctccctctc
     5461 gatcaactgc tttcttcctt ctgttttctt tccctgtgtc cgcggcgcct cggcggccga
     5521 tctttatttg ctgagcgcgg ggacgtatcc gtggccgggg atttcgcggc gcgggacggc
     5581 ggcggggagt tcgtcgccgc tggcggcggg aatctcgatg gagatgagga ggccgccgcg
     5641 cgggtcgttg gtcatctcca aactgtaggc ggcgccgtag agctgcgcga ggcgggcgcg
     5701 cgtgttggcc agcccgacgc cgtggccgtc gctcgtgatg tgcgcctcgt ccgcgggcag
     5761 gccggggccg ttgtcgccga cttcgaggcg cagcacgccc ccgcgccgcg cggcccgcac
     5821 ctcgacgcgc ccgggcgtcg agcgcagcgc gatgccgtgg cggatggcgt tctcgacgag
     5881 cggctggagg atgaggttgg ggacgcggca gttgagcgcc tgcggctcga tctcgacgcg
     5941 cgtggtcagg cggtcgtgga agcggacgcg ctcgatgtcg aggtagcagt tcatgaattc
     6001 cagctcctgc cggagcggca cctcctgcgt cgcggaggtt tcgagcgtga ggcggaggaa
     6061 gtcgcctaag cgcgcgatca tgcggtcggc ggcctccgcg tccgtgtgga cgagcgcgga
     6121 gatcgagtgc agcgcgttga agaggaagtg cgggtggagc tgcatcttca aagcctgaag
     6181 ctgcgcctgc gaggcgcgca gctcgccctc gcggtagcgc tggtagtagg tcgcggcgtg
     6241 gctgataagc acgatcaccc agtagaccag cagctccttg tcgagcatga agaaggtcag
     6301 gcggagcagc cggtcgagcg tgagcttgct gaagtctttc gagaaccagg tgtcgagcag
     6361 gatgtgtccc gcaccccacg ccaccgcgat cagcgtgccc gccgccgcgt gcacgagcac
     6421 cgcgcgccgc cagttcgccc gctcgatgcg gaagcgccgc gacagccaca gcacgagcgg
     6481 cgtcagcaac gcgaacagat agcaggcgcc catctgccac acgagcgcac gcgagaaggg
     6541 gatggggcgc tcgctgccgc tgcgcgaggc gacgacgtag agctggctgg cgaagaagag
     6601 gccgaccagc gtccacacgc cccagataaa gcttagccgc accaaccgct gccgccaatc
     6661 aacgcgcccg tcactcgtcg agtacaacat aacgcctcat catctcagca accatgttag
     6721 atgacaagcc gcacggttgc gatgcgaaaa aaacggcccc gcgaatttgg acgaggtacg
     6781 aaaagccgta cgcgggtctg gggcgtgggg cagcgggtgt cagcggctac agaccgagga
     6841 gcgcgccgag ggcctgccag acccgccgcg cctcttcgcc gtggacgacg atttcgttgg
     6901 tgccgggacg ccccgcggcg ggcgggttcg tgtcgaagac gatgctgaga acttcctgcc
     6961 cctccgtgtc ctgataggcc acgtactgga tgcggtcgag gttaatcagg agcgtcgccc
     7021 cgtcctcccg tgtcgcgctg acgaaaggca tcctaaactc ccagatacgc ctgataggtt
     7081 aagtaatcag gcatagtagg taatcaaacc tcgtgacaga gttaggctac ggctaccgca
     7141 cgcggcattt catcccacgt cttattttcc agaagccgtc ccgagcgttt tttgttcgtt
     7201 ccgccccatt gcttaaagaa aaaaggcacc ccggcagcct tacactgatc cttaacatct
     7261 actacccact caggacgcat tatgcgagca cctgggccag actcgccccc aacaatgacc
     7321 caatcaatgc cttttaggtt aaggttaggc agcggcccta gcagtggttc aagtgacagg
     7381 aacttaatgt gcgctcccgt ctcgcgcagg tggtcaatcc tgaacgtata atctttgctc
     7441 tcaacgctga cgcccatcca gacgtgaggc cgccattcaa gcgctgtgct taacctcaaa
     7501 agtcgttgcg accgcttcgt gaggacttga aaccgatgct gacaggcatg gttcattaca
     7561 ttaaaaactt tgtgaatgta atcagttgga acatctctgt ggaaaaggtc gctcatagag
     7621 ttgacaaaaa ttacctgcgg cgttttccag cgtaatggta actccaacat gcgttcctga
     7681 atcgtcagct cgaacccgtt cacgtagttg ggttgcccca tcgcccgcaa gcggagcgca
     7741 agccgttcgg catagcaatg gtcacagccg ggactaatct tcgtgcagcc tgttacggga
     7801 ttccaagttg actccatcca ttctattgtg gactttgacc ccattatatg gtgcctcgct
     7861 tacgaaactt atcaaagata tcgcgtatta tatttgctgc gacagctttg ttggaagcaa
     7921 aaaataggta atagacctct gctcctctag tattcctcat agggatcggt tcaggtacgt
     7981 aagagaatcc tgctacttcc ttaagcgtac tgtcacatct gagatgtgag tatttgtcac
     8041 atcggttttg ttagaatcgt tgaaccacgc tatctgtcgg aggttcaacg atgccacgtt
     8101 ctttgaaagt gc
//
LOCUS       DBNGOH010001192         4873 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_915150, whole genome shotgun sequence.
ACCESSION   DBNGOH010001192 DBNGOH010000000
VERSION     DBNGOH010001192.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 4873)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4873)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4873
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_915150"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..604)
                     /locus_tag="AC159P_23100"
     CDS             complement(<1..604)
                     /locus_tag="AC159P_23100"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HIR3698390.1"
                     /translation="MKTKLKKQLRIAVLVQHDLIPPDTLEGLGEAETVRIKTEFDVVS
                     TLKAAGHDVRPVGIYDDLNTINKAIEEHRPHVAFNLIEEFDSYPLFDQHVVSFLELKR
                     QKYTGCNPRGLTLSRDKALTKKILAYHRIHVPEFAVFARNRKVYRPKRLKFPLLVKSL
                     TAEGSAGIAQSSIVRDDEKLVERVEFIHRQTNSPAIAEQYI"
     gene            complement(601..1620)
                     /locus_tag="AC159P_23105"
     CDS             complement(601..1620)
                     /locus_tag="AC159P_23105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007327979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding metallopeptidase"
                     /protein_id="HIR3698391.1"
                     /translation="MRRFAWDEMTDEELLGLRLCDLPLRIEGTPLEERVERLYAELEE
                     RGLRFRPHVWLSEEWFSPDGVPGFAIPFYLAHPRLVKLERKQMLEVEGGSDKECMRIM
                     RHETAHALDNAYLFRRRKSWRDLFGPFALKYPESYMPDPTSRDYVLHLGAWYAQAHPA
                     EDWAETFAVWLASPRAVWRRRYLGWLALKKLEYVHRLMEEATSKPMKNSTRRRVEPLG
                     DIKKTLRDHYRRKRAHYGITWSTNFDRDLKRIFSNEPRHLTRPTAVKFLRGLRRELIQ
                     RIAEGTGVHHYTIDQLLLRVIERCKHLRLRVAGRQDHVKQQTLVMLTVQTMNVVHTGY
                     SRIAL"
     gene            complement(1791..3236)
                     /locus_tag="AC159P_23110"
     CDS             complement(1791..3236)
                     /locus_tag="AC159P_23110"
                     /inference="COORDINATES: protein motif:HMM:NF024910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HIR3698392.1"
                     /translation="MPTPSQQTITLIRGLTLIAAVSIIIGNVIGTGVFLKARVMTCNV
                     GTPGMVVTVWVVAGLLSLAGALTYAELAAMMPRAGGEYVFVREAYGSGASFLYGWSSF
                     FIAKTGSQASVAVAFAIFLNDLTGGALGAVYFTPNLFGYDFPLGTPQLIAVGLIAVIT
                     LLNCAAVVVSGWVATALTCVKIALVLGIGVGAFVLAEGDWGHYALANAGGACEGVDQA
                     ARLGVAGFGAAMLGALWGYDGWNNLTLVAGEVKNPQRNIPLALVGGTITIIILYVFVN
                     LAYFYVLDPTAVAGVSKTSSVAREVALKFLGPAAVTLMAAGLMASSVGTLHTSILTGA
                     RIPYAMARDGLFFQNLARLNPRTRVPVGALLVQGVWACILALSGSFDTLTDYVIFGSW
                     IFYGLVTASIFLFRRRLPHAERPYKAWGYPVVPVLFLLVTAFLLVNTLMTTPKQALAG
                     LGLIALGLPVYYFLFRHRRVTVAENLADDKN"
     gene            complement(3478..>4873)
                     /gene="lysA"
                     /locus_tag="AC159P_23115"
     CDS             complement(3478..>4873)
                     /gene="lysA"
                     /locus_tag="AC159P_23115"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR01048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HIR3698393.1"
                     /translation="APAWAIPGYLEAREGRLFIDGADSAALAREFDTPLFVFSERRVR
                     SNIGRLQEAAGRVGHPVRFFYASKANSTMGLLRVVRESGIDCEVNSGGELFKALRVGF
                     RPDQLIFNGTSKTPQEIDEAIGAGIYSINADSVYEIELIEARARALGKRARVALRLVP
                     EIGTRSHLGLQTALLTSKFGITSTEVLDAFRRALRHPDLLHVCGIHIHVGSQTPDVEP
                     FAAAFKTMWEHLKTIHDESGHTLEHINLGGGIPVNYLRDRSQAEDLPEHERDMLGAEL
                     EPADVLAAALRAARDSARDAAAEHLLDQVTILLEPGRSVIADAGLLLTTVRNIKRRPE
                     TGDVWILTDAGYNLVLSMNNYKWYYHLISAERADAPTEAGYKVAGPLCDSGDVYFDIE
                     RGGRLPDYRRLPANVRPGELLALLNTGAYTIAQMFPYNGRPLPAAVMVREGGHADLIR
                     RRDSYEDLLAGDVW"
BASE COUNT          800 a         1459 c         1789 g          825 t
ORIGIN      
        1 cgatgtactg ctcggcgatg gcgggcgagt tggtctggcg gtggatgaac tcgacgcgct
       61 cgaccagctt ctcgtcgtcg cggacgatgg acgactgcgc gatgccggcg ctcccttcgg
      121 cggtcaaaga cttgacgagc aaagggaact tcagccgctt cgggcggtag accttgcggt
      181 tgcgcgcgaa gacggcgaac tcggggacgt ggatgcggtg gtaggcgagg atttttttcg
      241 tcagcgcctt gtcgcgcgag agcgtcaggc cgcgcgggtt gcagcccgtg tacttctgcc
      301 gcttgagttc gaggaagctg acgacgtgct ggtcgaacaa ggggtagctg tcgaactcct
      361 cgatgaggtt gaaggcgacg tgcgggcggt gctcttcgat ggccttgttg atggtgttga
      421 ggtcgtcgta gatgccgacg ggccgcacgt cgtgcccggc ggccttgagc gtggagacca
      481 cgtcgaactc ggtcttgatg cggacggtct ccgcctcgcc caagccttcg agcgtgtcgg
      541 gcgggatcag gtcgtgctgg acgaggacgg cgatgcggag ctgctttttc agctttgtct
      601 tcatagggcg attcggctgt agcccgtgtg gacgacgttc atcgtctgga cggtgagcat
      661 gacgagcgtc tgctgcttga cgtgatcctg ccggcccgcg acgcgcagcc gcaggtgctt
      721 gcagcgctcg atcactctca ggagtagctg gtcgatcgtg tagtggtgaa cgcccgtgcc
      781 ctcggcgatg cgttggatca gctcgcggcg aaggccgcgc aggaacttga cggcggtcgg
      841 gcgcgtgagg tggcgcggct cgttcgagaa gatgcgtttg aggtcgcggt cgaagttcgt
      901 agaccacgtg ataccgtagt gcgcgcgctt gcggcggtag tggtcgcgca gcgtcttctt
      961 gatgtcgcct aagggttcga cgcggcgccg cgtcgagttc ttcatcggct tggaggtggc
     1021 ctcctccatc agccggtgga cgtattcgag ttttttgagg gcgagccagc cgaggtagcg
     1081 gcggcgccag acggcgcggg gagaggcgag ccagacggcg aaagtctcgg cccagtcctc
     1141 ggcggggtgg gcctgcgcgt accacgcgcc gaggtggagc acgtagtcgc ggctcgtcgg
     1201 gtcgggcatg taggactcgg ggtacttgag cgcgaagggg ccgaacaggt cgcgccagct
     1261 tttgcggcga cggaagaggt aggcgttgtc gagcgcgtgc gccgtctcgt ggcgcatgat
     1321 gcgcatgcac tctttgtccg agccgccctc gacttcgagc atctgcttgc gttcgagctt
     1381 cacaaggcgc gggtgcgcga ggtagaaggg gatggcgaag ccgggcacgc cgtcgggcga
     1441 gaaccactct tcggagagcc agacgtgcgg gcggaagcgc aggccgcgct cttcgagttc
     1501 ggcgtagagg cgttcgacgc gctcttcgag cggcgtgccc tcgatgcgca aaggcaggtc
     1561 gcacagacgc aggccgagca gctcttcgtc cgtcatctca tcccacgcga atcttctcac
     1621 cgccgccgtg accatgatgc ttcagataga gaggactgag gactgaggac tgaggactga
     1681 gtgaaaacat tgacgagtag cggacgatga gagcggtgct gtacggactc gtcagggttc
     1741 atgtcttttt ctgtcactcg gttctcagtc ctcagtgctt tggttaagcg tcaattcttg
     1801 tcgtccgcca gattctccgc gaccgtgacg cgccggtggc ggaagaggaa gtaatagacg
     1861 ggcaggccga gcgcgatcag gccgaggccg gcgagtgcct gtttgggcgt cgtcatcaaa
     1921 gtgttgacga gcaggaacgc cgtgacgagc aggaagagca cgggcacgac ggggtagccc
     1981 cacgctttgt aaggccgctc ggcgtggggg agcctgcggc ggaagaggaa gatcgaggcc
     2041 gtcaccagcc cgtagaatat ccacgagccg aagatgacgt aatcggtcag cgtgtcgaac
     2101 gagccggaga gcgcgaggat gcacgcccac acgccctgca ccagaagggc gccgacgggg
     2161 acgcgcgtgc gcgggttgag ccgcgcgagg ttctggaaga agaggccgtc gcgcgccatc
     2221 gcgtaaggga tgcgcgcgcc cgtcagaatc gaggtgtgca gcgtgccgac cgaggaggcc
     2281 atcagcccgg cggccatcag cgtgacggcg gcggggccga ggaacttgag cgcgacctcg
     2341 cgcgccaccg aggatgtttt ggagacgccg gcgaccgccg tcgggtcgag cacgtagaag
     2401 taggcgaggt tgacgaaaac gtagaggatg atgatcgtga tcgtgccgcc gacgagcgcg
     2461 agcgggatgt tgcgctgcgg gttcttcacc tcgcccgcga cgagcgtcag gttgttccac
     2521 ccgtcgtagc cccacagcgc gccgagcatc gccgccccga agcccgccac gccgagccgc
     2581 gccgcctgat cgacgccctc gcacgcgccg cccgcgttcg ccaacgcgta gtggccccag
     2641 tcaccttcgg cgagcacgaa ggcgcccacc ccgatgccca ggacgagcgc gatcttgacg
     2701 cacgtcagcg ccgtcgccac ccagccgctg acgacgactg ccgcgcagtt gaggagggtg
     2761 atgacggcga tcagcccgac ggctatcaac tgcggcgtgc cgagggggaa gtcgtagccg
     2821 aaaagattcg gcgtgaagta gaccgcgccg agcgcgccgc ccgtgaggtc gttgaggaag
     2881 atggcgaagg cgacggcgac cgaagcctgc gagccggtct tggcgatgaa gaagctcgac
     2941 cacccgtaga ggaagctcgc gcccgagccg taagcctcgc gcacgaagac gtactcgccg
     3001 cccgcgcgcg gcatcatcgc ggccagctcc gcgtaggtca gcgcgcccgc cagactgagc
     3061 agccccgcga cgacccacac cgtgacgacc atgcccggcg tcccgacgtt gcacgtcatc
     3121 acgcgcgcct tcagaaagac gcccgtgccg atgacgttgc cgatgatgat cgagacggcg
     3181 gcgatgagcg tcaggccgcg gatgagcgtg atggtctgct gtgatggtgt cggcatgcag
     3241 tgatgtctac cgcttcggtc gccgcggagc ggcgttcgag tttagcccgg tctttcaagg
     3301 ccgggtcaga cgtgcacgaa cgacgccgcg tcgcgtcagc gacgcctgaa tgggcgcggt
     3361 tcgattcacg cgtcgctacg cgacgcgaat gggtgtccta ttccaatccc ggccttgaaa
     3421 ggccgggcta cattcaaccg ccgctccgcg gcgcattacc tacgcgctcc gcatgaatca
     3481 ccaaacgtcg cccgccaata aatcctcgta cgagtcgcgc ctgcgaatca ggtcggcgtg
     3541 gccgccctcg cgcaccatga cggcggcggg caggggccgt ccgttgtagg ggaacatctg
     3601 cgcgatggtg taggcgccgg tgttgagcag cgcgagcagc tcgccggggc gcacgtttgc
     3661 ggggagcctc cggtagtcgg gcaggcgccc gccgcgctcg atgtcgaagt acacgtcgcc
     3721 ggaatcacag agcggcccgg ccaccttgta gcccgcttcc gtaggggcgt cggcgcgctc
     3781 ggcggagatg aggtggtagt accacttgta gttgttcatc gagagcacga ggttgtagcc
     3841 ggcgtcggtg agtatccaca cgtcgccggt ctcggggcgc cgcttgatgt tgcggacggt
     3901 ggtcaagagc agccccgcgt cggcgatgac gctgcggccc ggttcgagca ggatggtgac
     3961 ctggtcgagc aggtgctctg ccgcggcgtc gcgcgccgag tcgcgggccg cgcgcagggc
     4021 cgccgcgagc acgtccgcgg gttcgagttc ggcgccgagc atgtcgcgct cgtgctcggg
     4081 caaatcttcg gcctgcgagc ggtcgcgcag gtagttgacg gggatgccgc cgccgaggtt
     4141 gatgtgttcg agcgtgtggc ccgactcgtc gtggatcgtc ttcaggtgct cccacatcgt
     4201 cttgaaggcg gcggcgaacg gctccacgtc cggcgtctgc gagccgacgt ggatgtggat
     4261 tccgcagacg tggagcaggt cggggtggcg caaggcgcgg cggaaggcgt cgagcacttc
     4321 ggtcgaggtg atgccgaact tcgaggtgag gagcgcggtc tgcaagccga ggtgcgagcg
     4381 cgtgccgatt tcggggacga ggcggagcgc gacgcgggcg cgcttgccca gggcgcgggc
     4441 gcgtgcttca atcagctcga tctcgtagac ggaatcggcg ttgatggagt agatgccggc
     4501 cccgatggcc tcgtcgatct cctgcggggt cttgctcgtg ccgttgaaga taagctggtc
     4561 ggggcggaag ccgacgcgca gggccttgaa gagttcgccg cccgagttga cctcgcagtc
     4621 gatgccgctc tcgcggacga cgcggagcag ccccatcgtc gagttggcct tcgaggcgta
     4681 gaagaagcgg acggggtgtc cgacccggcc ggccgcctct tgcaggcgcc cgatgttcga
     4741 gcggacgcgc cgctcggaga agacgaagag cggcgtgtcg aactcgcgcg ccagtgcggc
     4801 cgagtccgcg ccgtcgatga agaggcgccc ttcgcgcgct tcgaggtaac cggggatcgc
     4861 ccacgccggg gcg
//
LOCUS       DBNGOH010001193         8692 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_1466162, whole genome shotgun sequence.
ACCESSION   DBNGOH010001193 DBNGOH010000000
VERSION     DBNGOH010001193.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 8692)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8692)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8692
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_1466162"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..277
                     /locus_tag="AC159P_23120"
     CDS             <1..277
                     /locus_tag="AC159P_23120"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR3698394.1"
                     /translation="ALAMRPDVLLFDEPTSALDPRRSAELRATLRGFVERGHTMIIVT
                     HSINFLEGLADHILYMESGEAVEYNEAERLMRDPRDPRTREFLSHAR"
     gene            326..1261
                     /locus_tag="AC159P_23125"
     CDS             326..1261
                     /locus_tag="AC159P_23125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HIR3698395.1"
                     /translation="MTRITPLFALLLFTAFGTHAQTQTPTPAATPTPSASPATPPAWL
                     DARVRAEVAAFSGNVRLYAKNLDTGAVYSLNGDERVRTASTIKVAVMVEAFARVAEGK
                     AKWSDELVLTKEKKVGGSGILNTFDDGLRLTFRDAVTLMMTLSDNTATNLVVDLLTAD
                     AVNARMDALGLKDTRLMRRVFGGGESAEGRREENKRFGLGRTSPREMVALMERLERGE
                     VVSPAASKEMLELMKREQGTNGIWRGLWRVPKATKSGALDALRSNVGLIYHPRGRIAL
                     AVTCDDMPEVNWTPDNPAFGLMSRLSEILIDGLGK"
     gene            1302..2177
                     /locus_tag="AC159P_23130"
     CDS             1302..2177
                     /locus_tag="AC159P_23130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020765875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3698396.1"
                     /translation="MANPARRLAENVGGDFFVDDSCIDCDACRQIAPATFRVHGEQSS
                     VYRQPRTDDEELRALKALVACPTSSIGTTEHHDYHAAIDAFPERIEDNVYFCGFTSEA
                     SFGAWSYLVVRPDAEGGNVLVDSPRYATQLVRKIEALGGVRTIFLTHRDDVADHERYA
                     RKFGAARVMHARDGAARYGVERAVEGDEAVRLDATLKAIPTPGHTRGHMVLLYRDKFL
                     FTGDHLAWSPTRGTLTAFRSVCWYSWPEQKRSMERLLDYRFEWVLPGHGRIHRDSTAG
                     MHEHLRACVEWMKGR"
     gene            complement(2274..3032)
                     /locus_tag="AC159P_23135"
     CDS             complement(2274..3032)
                     /locus_tag="AC159P_23135"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3698397.1"
                     /translation="MQKNALFCATGIFLGFAIGFFIANAVTRPGAPAASKAADTTETR
                     AAGPLDPTQSAGPLPPGHPEIGDAAGGTGGAPSATAASTSAEAQAAMSKADAAPKDFG
                     AQLAAGRTFYDLKDYKKAALYLERALAIKPKDFDALVLMGNARYDDSDLAGAAGFYER
                     ALEVNPNSPDVRTDFGNTFFMGTPPDYDRAIAEYRKSVALDPRHVNSWKNIASAAIRK
                     GDRATATEAVERLAALEPASAELPSFRQSIEALK"
     gene            3385..4908
                     /gene="resB"
                     /locus_tag="AC159P_23140"
     CDS             3385..4908
                     /gene="resB"
                     /locus_tag="AC159P_23140"
                     /inference="COORDINATES: protein motif:HMM:NF016995.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HIR3698398.1"
                     /translation="MSAIEETKREIGVAQAPAAAAPRGPSIITRALNLLSSVRLGVTL
                     LCVLVLFSMAGMLIMQQNVEGFDKYYASLTPAQQYLYGKLVLFDVYHAWYFNALLMLL
                     SLNIVLASIDRFPTAWTYISRKKLDASAHWLKGQEQHAELTMHAQGREALVNKISAAL
                     GAQGFKTRVSEKKGIAYVFGERGAWNRLGAYAVHVALLTIFFGGFLTAQFGRVGSMGL
                     APGQTSNEMVETVFNLDQPGQQTTELPFSVECTDIQQKLNRKEGPITADNTLDWLTYI
                     KIKDPQYGEREALVHMNRPYDYRGYRFFQASFIQPGRARHVYLRLTPAAGGEPLDVTI
                     KRDGTATLPDGTRLKFEDFYPDFGMSGGKPGTKTADYNNPAARLTVTGPSGQPENAYA
                     FQTELPESAPIGKPVAGYKFRLVDFEKVPNAHVLSVQRDPGATIVYVGFTLLGLTLAA
                     VFFFSHQRVWAHVEEREGGEFKVVLGGNTNRNKLGFGDRFRRVAAAISGQPIEVKES"
     gene            4905..6578
                     /gene="ccsA"
                     /locus_tag="AC159P_23145"
     CDS             4905..6578
                     /gene="ccsA"
                     /locus_tag="AC159P_23145"
                     /inference="COORDINATES: protein motif:HMM:NF013725.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HIR3698399.1"
                     /translation="MSEVLKQPAVIGTPELKWWNTFASYVPAILSVVGAVLLCLARVQ
                     TGPVGYIPNDGALMMLALAAYLVAAVFYLVNLYAPSSLFERIGLVGATLGVFFNLGSW
                     LVRWVAYREKEVAGWIQQGMPQSEWPWIFRNIPFANLYDLSLGFAFGAGITTLLIAHR
                     KNFRFLGAVSLPVASLILILARFIGDEFINLPPVLDSYWRPIHVGIAALSYGIALVCF
                     AVAVVYLLKDGVKTEAMAVWASIFALLVFGTISRGTIFGLGSAFWDFDLSTFALYRSS
                     VFLPPRFALPMRADIPYAGAAIFVAGLLLLGVVVAFCVYLFKQNESARKWGHHMLKVS
                     LVVQAIAIALLVSQVKSLTDVAVCQPGGPRPAGTACISESQFKILGAFIAQEQGLSPA
                     QIAAIEPQKLEFAAQQLIAERQGEMFLSLNANPVELAALITAFAATLFVTIFSFRTER
                     LRAALPSAEKIDSLMYKTAGVTFAGLALLLMTGAIWANESWGRYWGWDSKETGAFVAW
                     LTYGAFLHVRISRGWTGRAAAYFAIVAFLLVIFTYLGVSYLLPGLHSYA"
     gene            6665..7432
                     /locus_tag="AC159P_23150"
     CDS             6665..7432
                     /locus_tag="AC159P_23150"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3698400.1"
                     /translation="MNRENTLFAAVGLLLGYAAAFTLVVYLNQGPAPAARGGQAAGGA
                     ASSGQPMNAVKEQQRLESEAVAATQKARQEPENFEAQVAAANASVGTGDYEGAIDFLT
                     RANKLRPDDYDTLLMLGRANFEAQRYDVAGRWYTDALKRKPEDLDARSELALTYFLRT
                     PSDLDRAVTEFNATLARDPSHEPTLHNMTLVYIQTGKFSEAEAALARLEKASPQNDKL
                     PMLRERLREARGVSGNKPPGETSARGAAGAQKRTPSD"
     gene            7491..8252
                     /locus_tag="AC159P_23155"
     CDS             7491..8252
                     /locus_tag="AC159P_23155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3698401.1"
                     /translation="MRLHRRTPPFRTLLAFFALFTFAGVAAAQSPQPVCTPAAPPPTK
                     NDPAPPDATSGIRIAVTGPDGKPLQRKRFYLLERDVTKDGSFSAASAPKRDDFLAGAS
                     AELRAWLKKYDCDSLYCPEYEAGYEEAVKTVPEFKAAYEEGLKKYKNPKTAMRWLTVN
                     FPLKNVRTEYYNRKKAWLDAAAQKGGRVMSVMTDEKGVAYFTGVGLRTYYVSNLIPLE
                     DGGVLWNCEVAVPPPLPRQLYSISFTLTAPKAAAK"
     gene            8502..>8692
                     /locus_tag="AC159P_23160"
     CDS             8502..>8692
                     /locus_tag="AC159P_23160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3698402.1"
                     /translation="MKHRTATLAILLLLASVVFVFPRLTSAGASPAIEPQRRTTQRRR
                     QPARRPAQRRPRVDYSKFN"
BASE COUNT         1452 a         2975 c         2846 g         1419 t
ORIGIN      
        1 cgcgctggcg atgcgaccgg acgtgctcct cttcgacgag ccgaccagcg ccctcgaccc
       61 gcgccgcagc gccgagctgc gcgcgaccct gcgcggcttc gtcgagcggg ggcacacgat
      121 gatcatcgtc acgcactcga tcaacttcct cgaagggctg gccgaccaca tcctctacat
      181 ggagagcggc gaggccgtcg aatacaacga ggccgaacga ctcatgcgcg acccgcgcga
      241 cccgcgcaca cgcgaattcc tgtcgcacgc gcgatgagat gtgccgccgc tacgcggcga
      301 aaatccgaat ccttcttcag ataacatgac gcgaatcact ccactcttcg cgctcctctt
      361 gttcaccgcc ttcggcacgc acgcgcaaac acagacgccg acgcccgccg cgacaccgac
      421 gccgagcgct tcgcccgcga cgccgcccgc atggctcgac gcgcgggtgc gcgcggaggt
      481 cgcggcgttc agcgggaacg tccgtctcta cgcgaagaac ctcgacacgg gcgcggtcta
      541 ctctctgaac ggcgacgagc gcgtgcggac ggcgagcacg atcaaggtcg ccgtcatggt
      601 cgaagccttc gcgcgcgttg ccgaggggaa ggcgaagtgg tcggacgagc tggtgctgac
      661 gaaggagaag aaggtcggcg gctcgggcat cctcaacacc ttcgacgacg gcctgcggct
      721 caccttccgc gacgccgtga cgctgatgat gacgctcagc gacaacacgg cgaccaacct
      781 cgtcgtcgat ctcctgacgg cggacgcggt caacgcgcgg atggacgcgc tcgggctgaa
      841 agacacgcgg ctgatgcgta gggtcttcgg cggcggcgag agcgccgaag ggcggaggga
      901 agagaacaag cgcttcggcc tcgggcgcac cagcccgcgc gagatggtcg cgctcatgga
      961 gcggctggaa cgcggcgagg tcgtcagccc cgcggcctcg aaggagatgc ttgagctgat
     1021 gaagcgcgag cagggcacga acggcatctg gcgcgggctg tggcgcgtgc cgaaggcgac
     1081 caagtcgggc gcgctcgacg cgctcaggag caacgtcggc ctcatctacc acccgcgcgg
     1141 gcggatcgcg ctggccgtca cctgcgacga catgcccgaa gtcaactgga cgcccgacaa
     1201 ccccgccttc gggctgatgt cgcgcctctc ggagattctg atcgacggac tcggcaagtg
     1261 aacgtttggt gcgagggagc cggagacttt aaactcgggg catggcgaat ccggcgcggc
     1321 ggctcgcgga gaatgtcggg ggcgatttct tcgtcgatga cagttgcatc gactgcgacg
     1381 cgtgcaggca gatcgcgccc gcgacattcc gcgtccacgg tgagcagtcg agcgtctacc
     1441 gccagccacg gactgatgat gaggagctgc gtgcactgaa agctctcgtc gcgtgcccca
     1501 cctcttccat cggcaccacg gagcatcacg actaccacgc ggcgatcgac gccttccccg
     1561 aacgcatcga ggataacgtc tacttctgcg gcttcacgtc cgaggcgagc ttcggcgcgt
     1621 ggagctacct tgtcgtccgc cccgacgcgg agggcggcaa cgtcctcgtc gattcgccgc
     1681 gatacgccac gcagctcgtc aggaagatcg aggcgctcgg cggcgtccgc accatcttcc
     1741 tgacgcaccg cgacgacgtg gccgaccacg agcggtacgc gcgcaaattc ggcgccgcgc
     1801 gcgtgatgca cgcgcgggac ggcgcggcgc gctacggcgt cgagcgcgcg gtcgagggcg
     1861 acgaagcggt gcggctcgac gcgacgctca aggcgatccc gacgccgggc cacacgcgcg
     1921 gccacatggt gctgctctac agggacaagt ttctcttcac gggcgaccac ctcgcgtggt
     1981 cgccgacgcg cggcacgctg accgcgttca ggagcgtgtg ctggtactcg tggcccgagc
     2041 agaagcgctc gatggagcgc ctgctcgact accgcttcga gtgggtgctg cccggccacg
     2101 gtcgcattca tcgcgacagc acggcgggca tgcacgaaca cctgcgcgcg tgcgtcgagt
     2161 ggatgaaagg acgatgatga gtgcggaatg atgaacgatg aatcaaagac aggctgaagg
     2221 atgacatgca gcgtgcttct cattcatcat tccgcattcc gccttcatca tttctacttg
     2281 agcgcttcga tgctctgccg gaacgagggc agctccgcgc tggcgggttc gagcgcggcg
     2341 aggcgttcga cggcctcggt cgcggtcgcg cggtcgccct tgcggatggc ggcggaggcg
     2401 atgttcttcc agctattgac gtggcgcggg tcgagggcga cggacttgcg gtactcggcg
     2461 atggcgcggt cgtagtcggg cggggtgccc atgaagaagg tgttgccgaa gtccgtccgc
     2521 acgtcgggcg agttggggtt gacttcgagc gcgcgctcgt agaagcccgc ggcgccggcg
     2581 aggtcgctgt cgtcgtaacg agcgttgccc atcaggacga gcgcgtcgaa gtccttcggc
     2641 ttgatggcga gcgcgcgttc gaggtagagc gcggccttct tgtagtcctt caggtcgtag
     2701 aaggttctcc cggcggcgag ctgcgcgccg aagtctttcg gggcggcgtc ggccttcgac
     2761 atggccgcct gcgcctcggc ggaggtcgag gccgccgtgg ccgaaggggc gccgccggtg
     2821 ccgcccgcgg cgtcgccgat ctcggggtgg ccgggcggga gcgggccggc ggactgcgtc
     2881 gggtcgagcg ggccggcggc gcgcgtctcc gtggtgtcgg cggccttgct cgcggcgggg
     2941 gcgccggggc gcgtgacggc gttggcgatg aagaagccga tggcgaagcc gaggaaaatc
     3001 cccgtggcgc agaagagtgc gtttttttgc atgatggaaa gtaaagtgct cgtgatcgtc
     3061 cgccggagcg ggaagcggga tgataacaca aaatgcagtc ggcagtaggc agcgggcagc
     3121 gggcagccaa gacggaaata gacggaaagg gcggagaggc cggggacttt acctctcgaa
     3181 catcctcgcg cttcttgttt tgactgcctg ctgcccgctg cccgctgccc gctgccgact
     3241 gccgactgct atcggggttg ccgtcgttcg cacgtttggt tatgatgtgg cgcacggaag
     3301 gtgaagcgaa gccccacttg atagattgaa cgctctgaag tagtcgtctc cggttcaatt
     3361 taagtcccgt tttcattcga aacaatgtct gccatcgaag agaccaaaag agagatcggt
     3421 gtcgcgcagg caccggcggc ggcggcgcct cgggggccgt cgatcatcac gcgggcgctg
     3481 aacctgctca gctccgtgcg cctcggcgtg acgctgctgt gcgtgctggt cctgttctcg
     3541 atggccggca tgctcatcat gcagcagaac gtcgagggct tcgacaagta ctacgcgtcg
     3601 ctgacgcccg cgcagcagta cctctacggc aagctcgtcc tcttcgacgt gtaccacgcg
     3661 tggtacttca acgcgctgtt gatgctgctc tcgctcaaca tcgtgctcgc ctccatcgac
     3721 cgcttcccga ccgcgtggac gtacatcagc cgcaagaagc tcgacgcctc ggcccactgg
     3781 ctcaaggggc aggagcagca cgccgagctg acgatgcacg cacaggggcg cgaggcgctg
     3841 gtcaacaaaa tctccgccgc gctcggcgcg caggggttca agacgcgcgt ctcggagaag
     3901 aagggcatcg cctacgtctt cggcgagcgc ggcgcgtgga accgcctcgg cgcgtacgcc
     3961 gtccacgtcg cgctgctgac gatcttcttc ggcggctttt tgacggcgca gttcgggcgc
     4021 gtcgggagca tggggctagc gccgggccag acctcgaacg agatggtcga gaccgtcttc
     4081 aaccttgacc agccggggca gcagacgacc gagctgccgt tctcggtcga gtgcaccgac
     4141 atccagcaga aactcaaccg caaggaaggc ccgatcacgg cggacaacac gctcgactgg
     4201 ctgacctaca tcaagatcaa agaccctcag tacggcgagc gcgaagccct cgttcacatg
     4261 aaccgcccgt acgactaccg cggctaccgc ttcttccagg cgagcttcat ccagcccggt
     4321 cgcgcgcgcc acgtctacct gcggctgacg cccgccgcgg gcggcgagcc gctcgacgtg
     4381 acaatcaagc gcgacggcac ggcgacgctc cccgacggca cgcggctcaa gttcgaggac
     4441 ttctaccccg acttcggcat gagcggcggc aagcccggca cgaagaccgc ggactacaac
     4501 aatcccgccg cgcgcctcac cgtcaccggc ccctcggggc agcccgagaa cgcctacgcc
     4561 ttccagaccg agctgcccga gagcgcgccc atcggcaagc cggtcgcggg ctacaagttc
     4621 aggctcgtcg atttcgagaa ggtgcccaac gcgcacgtcc tctcggtgca gagagacccc
     4681 ggcgcgacca tcgtctacgt cggcttcacg ctgttggggc tgacgctcgc ggcggtcttc
     4741 ttcttctcgc accagcgcgt gtgggcgcac gtcgaggagc gcgagggcgg cgagttcaag
     4801 gtcgtcctcg gcggcaacac caaccgcaac aagctcggct tcggcgaccg cttccggcgc
     4861 gtcgccgccg ccatcagcgg acagcctatt gaggtgaagg aatcatgagt gaagtcctga
     4921 agcagccggc ggtgatcggc acgccggagt tgaagtggtg gaatactttc gcttcgtacg
     4981 ttccggcgat cctgtcggtc gtgggcgcgg tgctgctctg tctcgcgcgc gtccagaccg
     5041 gcccggtcgg gtacatcccc aacgacggcg cgctgatgat gctcgcgctc gccgcctacc
     5101 tcgtcgccgc cgtcttctac ctcgtcaacc tctacgcgcc gtcgtcgctc ttcgagcgca
     5161 tcggccttgt cggcgcgacg ctcggcgtct tcttcaacct cgggagctgg ctcgtccgct
     5221 gggtcgccta ccgcgagaag gaggtggccg ggtggataca gcaggggatg ccgcagagcg
     5281 agtggccgtg gattttccgc aacatcccgt tcgccaacct ctacgacctc tcgctcgggt
     5341 tcgccttcgg cgccggcatc acgacgctgc tcatcgcgca ccgcaagaac ttccgcttcc
     5401 tcggcgccgt ctcgctgccg gtcgcctcgc tgatcctgat cctcgcgcgc ttcatcggcg
     5461 acgagttcat caacctgccg cccgtgctcg actcgtactg gcgaccgatc cacgtcggca
     5521 tcgccgcgct ctcttacggc atcgcgctcg tctgcttcgc cgtcgcggtg gtctaccttt
     5581 tgaaggacgg cgtgaagacc gaggcgatgg ccgtctgggc gagcatcttc gcgctcctcg
     5641 tctttggcac catcagccgc ggcacgatct tcgggctggg gagcgccttc tgggacttcg
     5701 acctctcgac cttcgccctc taccgctcgt cggtcttcct gccgccgcgc ttcgcgctgc
     5761 cgatgcgcgc ggacatcccc tacgcgggcg cggccatctt cgtcgcgggg ctgctcctgc
     5821 tgggggtcgt cgtcgcgttc tgcgtctacc tcttcaagca gaacgagtcg gcgcgcaagt
     5881 ggggccatca catgctgaag gtctcgctcg tcgtgcaggc gattgccatc gcgctgctgg
     5941 tctcgcaggt caagtctttg acggacgtgg ccgtgtgcca gccgggcggc ccgcggcccg
     6001 ccgggacggc ctgcatcagc gagagccagt tcaaaatcct cggcgccttc atcgcgcagg
     6061 agcaggggct aagccccgcg cagatcgccg ccatcgaacc gcaaaagctt gagttcgccg
     6121 cgcagcagct catcgccgag cggcagggcg agatgttcct cagcctgaac gccaacccgg
     6181 tcgagctggc ggccttgatc acggcgttcg ccgccacgct cttcgtcacc atcttcagct
     6241 tccgcaccga gcggctgcgc gcggccttgc cctcggcgga gaagatcgac tcgctgatgt
     6301 acaagacggc gggcgtgacg ttcgccggac tcgcgctgct gctgatgacg ggcgccatct
     6361 gggccaacga atcgtggggc cgctactggg gctgggactc gaaggagacg ggcgccttcg
     6421 tcgcgtggct gacctacggc gccttcctcc acgtccgcat ctcgcgcggc tggacggggc
     6481 gcgccgcggc ctacttcgcc atcgtcgcct tcctcctcgt catcttcacc tacctcggcg
     6541 tcagctacct gctgccgggc ctgcactcgt acgcataaag agccgtgaac cgtaaaccgt
     6601 gataagtcag aaccggtttt tcttgtcacg gttcacggtt cacggttcac ggcgtttggt
     6661 gtttatgaat cgtgagaaca cgctctttgc cgccgtcggg ctgctgttgg gttacgcggc
     6721 ggccttcacg ctggtggtct atctgaacca ggggccggcg cccgcggcgc gcggcgggca
     6781 ggcggcgggc ggcgccgctt cgtcggggca gccgatgaac gccgtcaagg agcagcagcg
     6841 gctggagtcg gaggccgtcg cggcgacgca gaaggcgcgg caggagccgg agaacttcga
     6901 ggcgcaggtg gccgccgcca acgccagcgt cggcacgggc gattacgagg gcgcgatcga
     6961 tttcctgacg cgcgccaaca agctcaggcc cgacgactac gacacgctgc tcatgctggg
     7021 ccgcgcgaac ttcgaggcgc agcgctacga cgtggcgggg cgctggtaca ccgacgcgct
     7081 caagcgcaag cccgaagacc tcgacgcgcg ctccgagctg gcgctgacct acttcctccg
     7141 cacgccgtcc gacctcgacc gcgccgtcac cgagttcaac gccacgctcg cgcgcgaccc
     7201 ctcgcacgag ccgacgctcc acaacatgac gctcgtctac atccagacgg gcaagttctc
     7261 cgaggccgag gccgcgctcg cgcgcctcga gaaggccagc ccgcagaacg acaagctgcc
     7321 gatgctgcgc gagcgtctga gggaagcgcg cggcgtcagc ggcaacaagc cgccgggcga
     7381 gacctccgcc cgaggcgccg ccggcgcgca gaagagaacg ccgtccgact gattcgctgc
     7441 ttcctccgat gcgctccttg atccgaagac cacggcccgc aaagaaaatg atgagactgc
     7501 acaggcgcac gccccccttt cgcacgctcc tcgccttctt cgcgctattc actttcgcgg
     7561 gcgtcgccgc ggcccagtcg ccgcagcccg tctgcacgcc cgccgcgccg ccgcccacga
     7621 agaacgaccc ggccccgccc gacgccacca gcggcatccg cattgccgtc acaggccccg
     7681 acggcaagcc gctccagcgc aaacgcttct acctgctcga acgcgacgtg acgaaggacg
     7741 gcagcttcag cgcggcctcc gcgcccaaac gcgacgactt cctcgcgggc gccagcgcgg
     7801 agctacgcgc gtggctgaag aagtatgact gcgactcgct ctactgcccc gagtacgagg
     7861 ccggctacga ggaggccgtc aagaccgtcc ccgaattcaa ggccgcttac gaggaggggc
     7921 tgaagaagta caagaacccg aagaccgcca tgcggtggct gacggtcaac ttccccctga
     7981 agaacgtccg caccgaatac tacaaccgca aaaaggcatg gctcgacgcg gcggcgcaga
     8041 agggtggccg cgtgatgtcc gtgatgacgg acgagaaggg cgtcgcctac ttcaccggcg
     8101 tcgggctgcg cacctactac gtctcgaacc tcatcccgct cgaagacggg ggcgtcctct
     8161 ggaactgcga ggtggcggtg ccgccgccgc tcccgcgcca actctactcg atctccttca
     8221 cgctgaccgc gccgaaggcc gcggccaaat agcagacccc tcgccgaccg cggggcgcat
     8281 ttcgcgcacc cgcgccgcgc ggccccgtcc gacacgcgct cgctccgcac gggtcgccgc
     8341 aaaaatttgt tgtcagcgct tcgcttaatg tgctacaacc cgacgctacc gatcttcata
     8401 gccgttcgtt tgaatccgtc ggtcaggaat ccgatatcat ctccagaaaa cctattctcg
     8461 gagccgcgtc gggcgaagcc gaaaggcgct tcgtgaggac gatgaaacac cgcacagcaa
     8521 cgctcgccat cctgctcctg cttgcttcgg tcgtcttcgt tttcccacgg ctgaccagcg
     8581 cgggcgcgtc ccccgcgatc gaaccgcagc gccgcactac gcagcgccgc cgccagcccg
     8641 cgcgccgtcc cgcgcagcgc cgcccgcgcg tcgattactc gaagttcaac ca
//
LOCUS       DBNGOH010001194         3229 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_731861, whole genome shotgun sequence.
ACCESSION   DBNGOH010001194 DBNGOH010000000
VERSION     DBNGOH010001194.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3229)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3229)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3229
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_731861"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..752
                     /locus_tag="AC159P_23165"
     CDS             <1..752
                     /locus_tag="AC159P_23165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010493965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YqjF family protein"
                     /protein_id="HIR3698403.1"
                     /translation="ERPRGLPLMRQWWGKLLFMHWPVAPKLLRPLLPPQLTIDTFEGR
                     AWVGVVPFTMWGVRASLLPPVPGTSAFHELNVRTYVHYDGVPGVWFLSMDASNALAVW
                     GARRFYHLPYYNARMNLGGNARTVVYGSERTDRRAAPAAFGATWEIGEPLEQPEPDSL
                     TFFLTERYCLYSARREELFRCRIHHQPWPLRRATLTHHDSDVLEALGLPAPQGEPLLH
                     YAEAIKTDIWPLARVRTRIPAQVFEPTGAAL"
     gene            complement(916..2973)
                     /locus_tag="AC159P_23170"
     CDS             complement(916..2973)
                     /locus_tag="AC159P_23170"
                     /inference="COORDINATES: protein motif:HMM:NF025697.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4394 domain-containing protein"
                     /protein_id="HIR3698404.1"
                     /translation="MFQSARDPNDEIYVMNADGSGVVRLTSHPNGDIDPAVQPRAAAL
                     GVVRFSASAFTVSEGAGSVAVTVIRTGVVTGGLIVDLFTNSGTASERSDFTATFRTLE
                     FAPGETSKVVNIPVIDDAFLETDETFSVTLARATGGGLDSPSTAIVTITDNDSPPSPA
                     APVTIFAVTTTNNLLSFSSATPGTISSTTPITGLAPGESVVGLDVRPATRQLYGLVQR
                     AFAENTPPLRLVIINPTTGAATAVGTFFGPATSGISFGFDFNPTVDRIRITSDTDTNL
                     RVHPDTGAVAATDGALAYAAADANAGQNPNVVGSAYTNNFAGATTTTLYDIDSNLDIL
                     VTQNPPNNGTLNTVGALGVDASSLAGFDIATFSGTAFAGLNTGGAESGLYTINLTTGA
                     ATLVGQIGPSGTGTVRGLTVGNPFPNPIDDAAFFVRQQYLDFLNREPDAGGLAFWTND
                     LTRLIAECNAFADAEVRRQCVLGARAQISTAFFVSIEFQETGFFVIQVYLEAFGRLPT
                     FREFLEDLGAIREGVVIGQPGAFELLAANRSEYLDRFVDREDFRARYDGVSNADFVNA
                     LFTNAGVDPASEAATRDALVAGLNNGTETPETALLKVSETRSVYNAVFNRAFVLMEYF
                     GYLRRDPDPAGFAFWLAKLDSVSLPGEDVRDPGVALARIKRAQMVEAFIDSIEYRGRF
                     GPT"
BASE COUNT          497 a         1076 c         1117 g          539 t
ORIGIN      
        1 gcgagcgccc gcgcggcctg ccgctgatgc ggcagtggtg gggcaagctc ctcttcatgc
       61 actggccggt cgcgccgaag ctgctgcgcc cgctgctgcc gccgcagctc accatcgaca
      121 cgttcgaggg gcgcgcgtgg gtcggcgtcg tgcccttcac gatgtggggc gtgcgcgcct
      181 cgctgctgcc gcccgtcccc ggcacgagcg ccttccacga gctgaacgtc cgcacctacg
      241 tccactacga cggcgtgccc ggcgtctggt tcctctcgat ggacgcgagc aacgcgctcg
      301 ccgtctgggg cgcgcgcagg ttctatcacc tgccctacta caacgcgcgc atgaacctcg
      361 gcgggaacgc gcgcacggtc gtctacggct ccgagcgcac ggatcgccgc gccgcgcccg
      421 cggccttcgg cgcgacgtgg gagatcggcg agcctctgga gcagcccgaa cccgactcgc
      481 tcaccttctt cctcacggag cgctactgcc tctactcggc gcgccgcgag gagcttttcc
      541 gctgccgcat ccaccaccag ccgtggccgc tgcggcgcgc cacgctcacg caccacgact
      601 cggatgtcct cgaagcgctc ggcctgcccg cgccgcaagg cgaaccgctc ctccactacg
      661 ccgaagccat caagaccgac atctggcccc tcgcccgcgt ccgcacacga atccccgcac
      721 aggtcttcga gccgaccggc gcagcactct gaaggcagta ggcggtaggc agtaggcggt
      781 caagacagaa gggcggagag gcttgagagg taaagtgcaa agcctctccg ccccccaagc
      841 gataaagcaa gaggcttgag acgttgccgc tcaagcctct tgcttttact gcctaccgcc
      901 tactgcctac tcttcttacg ttgggccgaa gcgcccgcgg tactcgatcg agtcgatgaa
      961 tgcctcgacc atctgtgcgc gcttgatgcg ggccagagcc acacccggat cgcgcacgtc
     1021 ctcgcccggc aggctgacgc tgtccagctt ggcgagccag aaggcgaacc ctgccgggtc
     1081 ggggtcgcgc cggagatagc cgaagtactc catcagcacg aacgcgcggt tgaagacggc
     1141 gttgtagacc gacctcgtct cgctcacctt gagcagggcg gtctcgggcg tctccgtccc
     1201 gttgttcagg ccggcgacga gcgcgtcgcg cgtggcggct tccgaggccg ggtccacgcc
     1261 cgcgttggtg aacagcgcgt tgacgaagtc cgcgttcgag acgccgtcgt agcgcgcccg
     1321 gaagtcttcg cggtcaacga aacggtcgag gtactcgctg cggttcgccg ccagcagctc
     1381 gaaggcgccc ggctggccga tgacgacgcc ctccctgatc gcgcccaggt cttcgaggaa
     1441 ctcgcggaac gtcggcagac gcccgaaggc ttccaggtag acctgaatca cgaagaagcc
     1501 cgtctcctgg aactcgatgg agacgaagaa ggccgtcgag atttgcgcgc gcgcgccgag
     1561 gacgcactgg cgccgcacct cggcgtccgc gaaggcgttg cattcggcga tcaatctcgt
     1621 caggtcgttc gtccagaagg cgaggccgcc ggcgtccggc tcgcggttca ggaagtcgag
     1681 gtactgctgc ctgacgaaga aggccgcgtc gtcgatcggg ttggggaacg gattgccgac
     1741 cgtgaggccg cgcaccgtgc ccgtccccga cgggccgatc tgcccgacga gcgtcgcggc
     1801 gcccgtcgtc aggttgatcg tgtacagccc ggactcggcg ccgcccgtgt tcagcccggc
     1861 gaaggccgtg ccgctgaagg tggcgatgtc gaagccggcg aggctcgacg catcgacgcc
     1921 gagcgccccg accgtgttca gcgtcccgtt gttgggcggg ttctgcgtca cgaggatgtc
     1981 gaggttcgag tcgatgtcgt agagcgtggt cgtggtcgcc cccgcgaagt tgttggtgta
     2041 ggccgagccg acgacattcg ggttctgtcc cgcgttcgcg tcggcggcgg cataggcgag
     2101 cgcgccgtcg gtcgccgcga ccgcgcccgt gtcgggatgc acgcgcaggt tcgtgtccgt
     2161 gtcgctcgtg attcggattc gatccaccgt cgggttgaag tcgaagccga aggagatgcc
     2221 gctcgtggcg gggccgaaga aggtgccgac cgccgtcgcg gcgcccgtcg tcgggttgat
     2281 gatcacgagc cggagcggcg gcgtgttctc ggcgaacgcg cgctggacga ggccgtacaa
     2341 ctgcctcgtg gcgggccgga catcaagccc gacgacgctc tcgccggggg cgaggccggt
     2401 gatgggagtc gtgctgctga tcgtccccgg cgtcgcgctg ctgaagctca gcaggttgtt
     2461 cgtcgtcgtc acggcgaaga tcgtgacggg cgcggcgggc gagggcgggc tgtcgttgtc
     2521 ggtgatggtg acgatggccg tgctcgggct gtcgaggccg cccccggtcg ctctcgcgag
     2581 agtgaccgag aaggtctcgt cggtttcgag gaaggcgtcg tcgatgacgg ggatgttgac
     2641 gaccttgctc gtctcgccgg gcgcgaattc gagcgtcctg aaggtcgccg tgaaatcgga
     2701 gcgctccgag gccgtgccgg agttggtaaa gagatcgacg atcaggccgc cggtgacgac
     2761 gcccgtgcgg atcaccgtca cggcgacgct gcccgccccc tctgagacgg tgaaggcgga
     2821 ggcgctaaag cgcaccacgc cgagcgcggc ggcgcgcggc tggacggcgg ggtcgatgtc
     2881 gccgttcggg tggctggtga gccgcacgac gccgctgccg tcggcgttca tcacgtagat
     2941 ttcatcgttg gggtcgcgtg ccgattgaaa cacgatcttg gtgccgtcag gcgaccatgc
     3001 cggactgagg tccaggctgt agaccggtcc ggtgccttcg gcgaaaccaa tctgcacatt
     3061 cgtagtgagt cgaaccggat tactgccgtc agcacccatg acccagattt ccgcatttcg
     3121 ccccgcgcgg ttgctgatga aggcgatcct cgcaccgtca ggcgaccacg cgggctcgag
     3181 attcaacacc ggatcgatgg aattggttaa cggtacctga cccgtgccg
//
LOCUS       DBNGOH010001195         3072 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Pyrinomonadaceae bacterium isolate MAG05211
            SRR25158380_k127_4214493, whole genome shotgun sequence.
ACCESSION   DBNGOH010001195 DBNGOH010000000
VERSION     DBNGOH010001195.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414619
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pyrinomonadaceae bacterium (soil metagenome)
  ORGANISM  Pyrinomonadaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Blastocatellia;
            Blastocatellales; Pyrinomonadaceae.
REFERENCE   1  (bases 1 to 3072)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3072)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 21:19:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,637
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,548
            CDSs (with protein)               :: 4,548
            Genes (RNA)                       :: 33
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 3 of 56
            Pseudo Genes (incomplete)         :: 48 of 56
            Pseudo Genes (internal stop)      :: 7 of 56
            Pseudo Genes (multiple problems)  :: 2 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3072
                     /organism="Pyrinomonadaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158380_k127_4214493"
                     /isolate="MAG05211"
                     /isolation_source="soil"
                     /db_xref="taxon:2283092"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(55..1527)
                     /locus_tag="AC159P_23175"
     CDS             complement(55..1527)
                     /locus_tag="AC159P_23175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002636571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HIR3698405.1"
                     /translation="MRKRLSISRGAALACAVVCVLVAAQAQVFAQADKAAQIDEMMKT
                     YHGYRQFNGAVLVAEGGKVIHKKGYGMANMEWNIPVAADTRFRLGSITKQFTAALVLQ
                     LVQEGKVKLDGHVTDYLPDYRKDTGARVTIHQLLNHTSGIPSYTGLPGFFKDVSRNPY
                     AVGDFVKQYASGDLEFEPGTKFNYNNSGYFLLGAIIEKVTGKPYEAALKERIFDPVGM
                     KDTGYDHHETILAKRAAGYQKTPAGFVNAPYLDMSLPYAAGSLYSTVEDLYLWDQALY
                     TDKLLSAQYKELMFKPGLRDYAYGWGVRKVALGPGKQIQVIRHDGGINGFNTLIMRMP
                     ETKSLVVLLNNTGGAKLDEMAGKIAAVLHGLPHRAPLRDISEALFNTVTEKNVEAAVK
                     QYRDLKAAQAADYDFSEPQLNTLGYQLLGMKRVKDAIEIFKLNVEMFPQGFNTYDSLG
                     EAYMVNGDKELAIQNYKKSLELNPQNTGATAALKKLEAKP"
     gene            complement(1789..2445)
                     /locus_tag="AC159P_23180"
     CDS             complement(1789..2445)
                     /locus_tag="AC159P_23180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3698406.1"
                     /translation="MKASNDAPPREAFTAREWKVIRTHRTPERVQRYLSRLKYNREEC
                     GATLRSFREVVRRGEAHCLEAALAAAVMLEQHGYPPLLLSLESQDYLDHVIFVFRRDG
                     LWGAVARSRDLGLHGRRPVFRTLRQLVWNYFDTYVDHTGRITGYGLANLHDLGGYDWR
                     FSPRNVWKVERHLQRIPHRPLRSSDRRYARLLARYKEFRRHHPTGHLTDYESRDLWML
                     "
     gene            2739..2981
                     /locus_tag="AC159P_23185"
     CDS             2739..2981
                     /locus_tag="AC159P_23185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3698407.1"
                     /translation="MSKKGSTTTPRQLTRAEREVQTAAERLTVQIEDALAAITVHAPE
                     SGDDLDACADRLERAARDMAVAIRELAEQRGGSLSQ"
BASE COUNT          509 a          958 c         1001 g          604 t
ORIGIN      
        1 taagcattgc gtcttatctc ccccactccc tttctcccct tctcccctac tgccttacgg
       61 cttcgcttcg agcttcttca gcgccgcggt ggcgcccgtg ttctgcgggt tcagctcaag
      121 cgacttcttg tagttctgga tggcgagttc tttgtcgccg ttgaccatgt aggcttcgcc
      181 gaggctgtcg taggtgttga agccctgcgg gaacatctcg acgttgagct tgaagatttc
      241 gatggcgtcc ttgacgcgct tcatgccgag gagctggtag ccgagggtgt tgagctgcgg
      301 ctccgagaag tcgtagtcgg cggcctgcgc cgccttcagg tcgcggtact gcttgacggc
      361 ggcctcgacg ttcttctccg tgacggtatt gaagagcgct tcggagatgt cgcgcagcgg
      421 cgcgcggtgc ggcagcccgt ggaggacggc ggcgattttg cccgccatct cgtcgagctt
      481 cgccccgccc gtgttgttga ggagcacgac gaggctcttc gtctcgggca tccgcatgat
      541 gagcgtgttg aagccgttga tgccgccgtc gtgacggatg acctgaatct gcttgccggg
      601 gccgagcgcc accttcctca cgccccagcc gtaggcgtag tcacgtagtc ccggcttgaa
      661 catcagctcc ttgtactgcg ccgacaaaag cttgtccgtg tagagcgcct gatcccacag
      721 gtagaggtct tcgacggtcg agtagagcga gcctgccgcg taagggagcg acatgtcgag
      781 gtagggcgcg ttgacgaagc ccgcgggcgt cttctgataa ccggcggcgc gcttcgccag
      841 aatcgtttcg tggtggtcgt agcccgtgtc cttcatcccg acggggtcga agatgcgctc
      901 cttgagcgcg gcctcgtagg gcttgcccgt gaccttctcg atgatcgcgc cgaggaggaa
      961 gtagcccgag ttgttgtagt tgaacttcgt ccccggctcg aactcaaggt cgccgctcgc
     1021 gtactgcttg acgaagtcgc cgaccgcgta ggggttgcgg ctcacgtcct tgaagaagcc
     1081 gggcaggccc gtgtagctcg ggatgccgga ggtgtggttc aggagttggt ggatggtgac
     1141 gcgcgcgccc gtgtccttgc ggtagtcggg caggtagtcc gtgacgtgcc cgtcgagctt
     1201 gaccttgccc tcctgcacga gttgcaggac gagcgccgcc gtgaactgct tcgtgatcga
     1261 gccgaggcgg aacctcgtgt ccgccgccac ggggatgttc cactccatgt tcgccatccc
     1321 gtagcccttc ttgtggatga ccttgccgcc ctccgcgacc agcaccgcgc cgttgaactg
     1381 ccggtagccg tggtaggtct tcatcatctc gtcgatctgg gcggccttgt cggcctgcgc
     1441 gaagacttgc gcctgcgcgg cgaccaacac gcagaccact gcgcaggcga gcgccgcccc
     1501 gcgcgatatc gaaagacgct ttctcataaa ccgattcttc tccgtcgttg gaatattcag
     1561 cgctgccgga caggttagga tacgttctgc ggtcaagaag gttgcaagaa ggaaaaggtt
     1621 aaaaggttaa aaggggaaaa gggaagacga gaaacagcgt gcctctcatc aaccctcgat
     1681 gagaggcacg ctgtttctcg ttactcaacc gaggatgaga agcagcgtac ttgttatcct
     1741 cccttttccc ttttcgccct ttcccctttc aaccttttcg ccctttcttc acagcatcca
     1801 caggtcgcgg ctctcgtagt cggtgaggtg gcctgtgggg tggtgtcggc ggaactcttt
     1861 gtagcgcgcg agcaggcgcg cgtagcggcg gtcggaggag cgcaaggggc ggtgcggaat
     1921 ccgttgcagg tggcgctcga ccttccagac gttgcggggc gagaagcgcc agtcgtagcc
     1981 gccgaggtcg tggaggttgg cgaggccgta gccggtgatg cggcccgtgt gatcgacgta
     2041 ggtgtcgaag tagttccaca cgagctggcg cagggtgcgg aagacggggc gccgcccgtg
     2101 cagcccgagg tcgcgcgagc gcgcgaccgc accccacaaa ccgtccctcc gaaaaacgaa
     2161 gatgacgtgg tcgagatagt cctgcgattc gaggctcaac aggagcggcg ggtagccgtg
     2221 ctgctcgagc atcaccgccg ccgcgagcgc cgcctcgagg cagtgcgcct cgccgcgccg
     2281 caccacctcg cggaacgagc gcagcgtcgc gccgcactcc tcgcggttat acttcagccg
     2341 cgacaggtag cgctgcacgc gctccggcgt gcggtgcgtc cggatgactt tccactcccg
     2401 cgcggtgaac gcctcccgcg gcggcgcgtc gttgctggcc ttcatcgatg tgtaaaaaat
     2461 ggaagtccgc ggcgagcctc aagcgcgggc gcatcttaac agcgcggggg cgcgacacga
     2521 aaacggaggg cggcgattcg agcgtgcgct tccgccccga gtcgcccacc cgacttgaaa
     2581 gcgcgcgccg ttggtgttaa gacattagct cgttcggagg cattctggta ttttggacag
     2641 gcgctcttgc cgctctgtgt gcaacccgcg cttcataatt ttcgagacga gaagtgccgg
     2701 ctttccccga ccgccgcggc acgctggact aaatgaccgt gagcaaaaag ggcagtacca
     2761 cgacaccgag gcagttgacc cgcgccgagc gcgaggtgca gaccgccgcc gagcgcctga
     2821 ccgtgcagat cgaggacgcg ctggccgcga tcaccgtgca cgcgcccgag agcggcgacg
     2881 acctcgacgc ctgtgccgac cgactggaac gcgccgcccg cgacatggcc gtcgccatcc
     2941 gcgagctggc cgaacagcgc ggcggaagtc tttcgcagta aaaacttcaa ggggccgaca
     3001 gtttcatcaa ccatcctgcg ggagcgcccg cgcgtacctt gtctaaggcc cgcggcgctc
     3061 ctgccgcacg tt
//
